LOCUS       DACAPB010000001        91930 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.001, whole genome shotgun sequence.
ACCESSION   DACAPB010000001 DACAPB010000000
VERSION     DACAPB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.001"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            34..531
                     /locus_tag="IT904_000001"
     CDS             34..531
                     /locus_tag="IT904_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rhsA"
                     /protein_id="HAP3189845.1"
                     /translation="MRGRYITQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPL
                     PIPWPKSPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVA
                     DTGITDRVNDIINDRFWSDGKKPDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQS
                     NDKKR"
     gene            552..1394
                     /locus_tag="IT904_000002"
     CDS             552..1394
                     /locus_tag="IT904_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HAP3189846.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1547..2380
                     /locus_tag="IT904_000003"
     CDS             1547..2380
                     /locus_tag="IT904_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAP3189847.1"
                     /translation="MDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNL
                     LSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMT
                     AKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGR
                     GTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKSSDI
                     R"
     gene            2392..2853
                     /locus_tag="IT904_000004"
     CDS             2392..2853
                     /locus_tag="IT904_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP3189848.1"
                     /translation="MKAYLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            3560..3997
                     /locus_tag="IT904_000005"
     CDS             3560..3997
                     /locus_tag="IT904_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3189849.1"
                     /translation="MADKAILLALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(4457..5593)
                     /locus_tag="IT904_000006"
     CDS             complement(4457..5593)
                     /locus_tag="IT904_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP3189850.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5596..5958)
                     /locus_tag="IT904_000007"
     CDS             complement(5596..5958)
                     /locus_tag="IT904_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAP3189851.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6495..8408
                     /locus_tag="IT904_000008"
     CDS             6495..8408
                     /locus_tag="IT904_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP3189852.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLDRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8638..9786
                     /gene="mtlD"
                     /locus_tag="IT904_000009"
     CDS             8638..9786
                     /gene="mtlD"
                     /locus_tag="IT904_000009"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP3189853.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9786..10373
                     /gene="mtlR"
                     /locus_tag="IT904_000010"
     CDS             9786..10373
                     /gene="mtlR"
                     /locus_tag="IT904_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAP3189854.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10385..10594)
                     /locus_tag="IT904_000011"
     CDS             complement(10385..10594)
                     /locus_tag="IT904_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3189855.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10879..11241
                     /locus_tag="IT904_000012"
     CDS             10879..11241
                     /locus_tag="IT904_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAP3189856.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
     gene            11887..12468
                     /locus_tag="IT904_000013"
     CDS             11887..12468
                     /locus_tag="IT904_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP3189857.1"
                     /translation="MKQEEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     SIPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMTSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPAADNRQKRSKK"
     gene            12512..17140
                     /locus_tag="IT904_000014"
     CDS             12512..17140
                     /locus_tag="IT904_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3189858.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYDGQGVDYGDGSASDGWVAIGKGAKANIFLNNAGASTALGYDAIAEG
                     QYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSFGQYSMALGRYSKALGRL
                     SIAMGDSSKADGANAIALGNAAKAAGIMSIGLGDNANASQDYAMALGAESEAAENATA
                     IGNKAHAKGVNSIALGNGSQALADSAIAIGQGNKANGADAIALGNGSQSSGLNAIALG
                     KASVVTGDNSLAFGSNTNANGINSVALGAGSIADQDDSVSVGSDSLQRKIVNVKNGTI
                     KADSHDAINGSQLYAISDSVAKRLGGGSSVNVDDGTVKAPTYNLKNGNKNNVGDALTV
                     LDQFTLQWDQNRDKYSAAHGSSTASVITDVADGAVSDSSKDAVNGSQLKATNDDVETN
                     TTNIATNTGNIATNTANIATNTTNITNLTDTVGDLKNDALLWNGTAFNAAHGTETTST
                     ITNVKAGTLSDDSTDAVNGSQLKATNDNVATNTTNIASNTANIATNTANINTLNTSID
                     TLEQDAILWNGTAYSAAHGTETASTITNVKAGTLSENSTDAVNGAQLNATNANVATNT
                     TNIATNTASINTLNTSIDALEQDALLWDGTAFSAAHGANKDASKITNVLAGTVSSAST
                     DAINGSQLHGLSSSIATYLGGGATVSDSGVFSGPTYNIDGNDYTNVGAALDAINTSLS
                     DSLGDALLWDSTTGAFSAKHGSTASVITNVADGAVSDSSSDAVNGSQLYDVSNSVVDV
                     LGGGAGVNTDGSISAPTYTIANTDYDNVGDALNALDTTLDDAMLWDATAGENGAFSAS
                     HDGSASKITNVAAGTISDTSTDAVNGAQLHGVSSSVAEALGGGAAVNSDGSISAPTYT
                     IADTDYTNVGDAMNAIDSTLDNALLWDATAGENGAFNASHDGKASVITNVANGQINET
                     STDAVNGSQLNATNMLIQNIAGDTSESYITENGEGINYVRTNDSGLVFEDASATGVGA
                     TAVGYNSVASGDSSVAIGQNSSSTIESGIALGSSSVSNRVILQGSRDTSVTEDGVVIG
                     YNTSDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPSKYFHA
                     NSTEEDSLAVGEDSLAMGAKTIVNGDAGIGIGLNTLVLTDAINGIAIGSNASANHANS
                     IAMGSGSQTTRGAQTDYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNV
                     GQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVD
                     ANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGKNAT
                     DAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNY
                     AQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASI
                     GGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            17508..19163
                     /gene="lldP"
                     /locus_tag="IT904_000015"
     CDS             17508..19163
                     /gene="lldP"
                     /locus_tag="IT904_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAP3189859.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            19163..19939
                     /gene="lldR"
                     /locus_tag="IT904_000016"
     CDS             19163..19939
                     /gene="lldR"
                     /locus_tag="IT904_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAP3189860.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            19936..21126
                     /gene="lldD"
                     /locus_tag="IT904_000017"
     CDS             19936..21126
                     /gene="lldD"
                     /locus_tag="IT904_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAP3189861.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            21279..21752
                     /gene="trmL"
                     /locus_tag="IT904_000018"
     CDS             21279..21752
                     /gene="trmL"
                     /locus_tag="IT904_000018"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP3189862.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(21805..22626)
                     /gene="cysE"
                     /locus_tag="IT904_000019"
     CDS             complement(21805..22626)
                     /gene="cysE"
                     /locus_tag="IT904_000019"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP3189863.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(22706..23725)
                     /gene="gpsA"
                     /locus_tag="IT904_000020"
     CDS             complement(22706..23725)
                     /gene="gpsA"
                     /locus_tag="IT904_000020"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP3189864.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(23725..24192)
                     /gene="secB"
                     /locus_tag="IT904_000021"
     CDS             complement(23725..24192)
                     /gene="secB"
                     /locus_tag="IT904_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAP3189865.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(24255..24506)
                     /gene="grxC"
                     /locus_tag="IT904_000022"
     CDS             complement(24255..24506)
                     /gene="grxC"
                     /locus_tag="IT904_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAP3189866.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(24648..25079)
                     /locus_tag="IT904_000023"
     CDS             complement(24648..25079)
                     /locus_tag="IT904_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP3189867.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            25324..26868
                     /gene="gpmM"
                     /locus_tag="IT904_000024"
                     /gene_synonym="pgmI"
     CDS             25324..26868
                     /gene="gpmM"
                     /locus_tag="IT904_000024"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAP3189868.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            26878..28161
                     /gene="envC"
                     /locus_tag="IT904_000025"
     CDS             26878..28161
                     /gene="envC"
                     /locus_tag="IT904_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAP3189869.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            28165..29124
                     /locus_tag="IT904_000026"
     CDS             28165..29124
                     /locus_tag="IT904_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAP3189870.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(29111..30145)
                     /gene="waaH"
                     /locus_tag="IT904_000027"
     CDS             complement(29111..30145)
                     /gene="waaH"
                     /locus_tag="IT904_000027"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAP3189871.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKYYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKIMIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(30384..31409)
                     /gene="tdh"
                     /locus_tag="IT904_000028"
     CDS             complement(30384..31409)
                     /gene="tdh"
                     /locus_tag="IT904_000028"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP3189872.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(31419..32615)
                     /gene="kbl"
                     /locus_tag="IT904_000029"
     CDS             complement(31419..32615)
                     /gene="kbl"
                     /locus_tag="IT904_000029"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP3189873.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFRGQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            32829..33761
                     /gene="rfaD"
                     /locus_tag="IT904_000030"
     CDS             32829..33761
                     /gene="rfaD"
                     /locus_tag="IT904_000030"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAP3189874.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            33771..34817
                     /gene="rfaF"
                     /locus_tag="IT904_000031"
     CDS             33771..34817
                     /gene="rfaF"
                     /locus_tag="IT904_000031"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAP3189875.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            34821..35792
                     /gene="rfaC"
                     /locus_tag="IT904_000032"
     CDS             34821..35792
                     /gene="rfaC"
                     /locus_tag="IT904_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAP3189876.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(35862..37115)
                     /locus_tag="IT904_000033"
     CDS             complement(35862..37115)
                     /locus_tag="IT904_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP3189877.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLVTPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKISGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFITIILLCAYFKAQSDQCLLEK"
     gene            complement(37161..38144)
                     /locus_tag="IT904_000034"
     CDS             complement(37161..38144)
                     /locus_tag="IT904_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP3189878.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHEKIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     QLNQLVESNEKELKDCNLPGKRDKRRAKILASKNYIIWRMVRLVNRKKNKR"
     gene            complement(38229..39254)
                     /locus_tag="IT904_000035"
     CDS             complement(38229..39254)
                     /locus_tag="IT904_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HAP3189879.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQNYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(39280..39972)
                     /gene="rfaY"
                     /locus_tag="IT904_000036"
     CDS             complement(39280..39972)
                     /gene="rfaY"
                     /locus_tag="IT904_000036"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAP3189880.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(39982..40977)
                     /locus_tag="IT904_000037"
     CDS             complement(39982..40977)
                     /locus_tag="IT904_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HAP3189881.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDNYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            complement(40994..42010)
                     /gene="waaO"
                     /locus_tag="IT904_000038"
                     /gene_synonym="rfaI"
     CDS             complement(40994..42010)
                     /gene="waaO"
                     /locus_tag="IT904_000038"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAP3189882.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSHLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDEIVLIHYVGPTKPWHYW
                     ASYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            complement(42026..42823)
                     /gene="rfaP"
                     /locus_tag="IT904_000039"
     CDS             complement(42026..42823)
                     /gene="rfaP"
                     /locus_tag="IT904_000039"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAP3189883.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEIFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(42816..43940)
                     /locus_tag="IT904_000040"
     CDS             complement(42816..43940)
                     /locus_tag="IT904_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP3189884.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFTWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(43937..44995)
                     /gene="rfaQ"
                     /locus_tag="IT904_000041"
     CDS             complement(43937..44995)
                     /gene="rfaQ"
                     /locus_tag="IT904_000041"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAP3189885.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTSLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            45408..46685
                     /gene="waaA"
                     /locus_tag="IT904_000042"
     CDS             45408..46685
                     /gene="waaA"
                     /locus_tag="IT904_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAP3189886.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRLTLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            46693..47172
                     /gene="coaD"
                     /locus_tag="IT904_000043"
     CDS             46693..47172
                     /gene="coaD"
                     /locus_tag="IT904_000043"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP3189887.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(47211..48020)
                     /gene="mutM"
                     /locus_tag="IT904_000044"
     CDS             complement(47211..48020)
                     /gene="mutM"
                     /locus_tag="IT904_000044"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAP3189888.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(48118..48285)
                     /gene="rpmG"
                     /locus_tag="IT904_000045"
     CDS             complement(48118..48285)
                     /gene="rpmG"
                     /locus_tag="IT904_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP3189889.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(48306..48542)
                     /gene="rpmB"
                     /locus_tag="IT904_000046"
     CDS             complement(48306..48542)
                     /gene="rpmB"
                     /locus_tag="IT904_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP3189890.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(48759..49427)
                     /locus_tag="IT904_000047"
     CDS             complement(48759..49427)
                     /locus_tag="IT904_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP3189891.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            49599..50819
                     /gene="coaBC"
                     /locus_tag="IT904_000048"
     CDS             49599..50819
                     /gene="coaBC"
                     /locus_tag="IT904_000048"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAP3189892.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            50797..51255
                     /gene="dut"
                     /locus_tag="IT904_000049"
     CDS             50797..51255
                     /gene="dut"
                     /locus_tag="IT904_000049"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP3189893.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            51362..51958
                     /gene="slmA"
                     /locus_tag="IT904_000050"
     CDS             51362..51958
                     /gene="slmA"
                     /locus_tag="IT904_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAP3189894.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(51995..52636)
                     /gene="pyrE"
                     /locus_tag="IT904_000051"
     CDS             complement(51995..52636)
                     /gene="pyrE"
                     /locus_tag="IT904_000051"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP3189895.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(52702..53418)
                     /gene="rph"
                     /locus_tag="IT904_000052"
     CDS             complement(52702..53418)
                     /gene="rph"
                     /locus_tag="IT904_000052"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP3189896.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            53545..54408
                     /locus_tag="IT904_000053"
     CDS             53545..54408
                     /locus_tag="IT904_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP3189897.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            54629..55006
                     /locus_tag="IT904_000054"
     CDS             54629..55006
                     /locus_tag="IT904_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3189898.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFR"
     gene            55046..55453
                     /locus_tag="IT904_000055"
     CDS             55046..55453
                     /locus_tag="IT904_000055"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3189899.1"
                     /translation="MKEHNKQLVEAAQQAGVATATDFAIFQNHGYQGLYGGLDQKAIH
                     QLKGLKKSQKILDHMGSTELAANLFRATQTEEKLKRDGVNSKQQANTTHFDVGSKVRQ
                     TIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            55744..56361
                     /locus_tag="IT904_000056"
     CDS             55744..56361
                     /locus_tag="IT904_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAP3189900.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(56358..58026)
                     /gene="ligB"
                     /locus_tag="IT904_000057"
                     /pseudo
     CDS             complement(56358..58026)
                     /gene="ligB"
                     /locus_tag="IT904_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            58284..58907
                     /gene="gmk"
                     /locus_tag="IT904_000058"
     CDS             58284..58907
                     /gene="gmk"
                     /locus_tag="IT904_000058"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP3189901.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            58962..59237
                     /gene="rpoZ"
                     /locus_tag="IT904_000059"
     CDS             58962..59237
                     /gene="rpoZ"
                     /locus_tag="IT904_000059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP3189902.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            59256..61364
                     /gene="spoT"
                     /locus_tag="IT904_000060"
     CDS             59256..61364
                     /gene="spoT"
                     /locus_tag="IT904_000060"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP3189903.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            61371..62060
                     /gene="trmH"
                     /locus_tag="IT904_000061"
     CDS             61371..62060
                     /gene="trmH"
                     /locus_tag="IT904_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAP3189904.1"
                     /translation="MNPTRYVRICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            62066..64147
                     /gene="recG"
                     /locus_tag="IT904_000062"
     CDS             62066..64147
                     /gene="recG"
                     /locus_tag="IT904_000062"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP3189905.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDLALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVLFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(64316..65521)
                     /gene="gltS"
                     /locus_tag="IT904_000063"
     CDS             complement(64316..65521)
                     /gene="gltS"
                     /locus_tag="IT904_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAP3189906.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            65801..67192
                     /gene="xanP"
                     /locus_tag="IT904_000064"
     CDS             65801..67192
                     /gene="xanP"
                     /locus_tag="IT904_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAP3189907.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            67313..69022
                     /locus_tag="IT904_000065"
     CDS             67313..69022
                     /locus_tag="IT904_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP3189908.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(69075..71392)
                     /gene="yicI"
                     /locus_tag="IT904_000066"
                     /pseudo
     CDS             complement(69075..71392)
                     /gene="yicI"
                     /locus_tag="IT904_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
     gene            complement(71402..72784)
                     /locus_tag="IT904_000067"
     CDS             complement(71402..72784)
                     /locus_tag="IT904_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAP3189909.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            73077..73171
                     /locus_tag="IT904_000068"
     tRNA            73077..73171
                     /locus_tag="IT904_000068"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:73111..73113,aa:Sec,seq:tca)
     gene            73280..73426
                     /locus_tag="IT904_000069"
     CDS             73280..73426
                     /locus_tag="IT904_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP3189910.1"
                     /translation="MDDERLKNPPVGSSVLERIRDIHASMRRVYLQVICPPADSQPTV
                     SPLR"
     gene            73808..74992
                     /locus_tag="IT904_000070"
     CDS             73808..74992
                     /locus_tag="IT904_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HAP3189911.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAIICLLFIK
                     DI"
     gene            75103..76026
                     /locus_tag="IT904_000071"
     CDS             75103..76026
                     /locus_tag="IT904_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP3189912.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLSVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(76030..76848)
                     /gene="nlpA"
                     /locus_tag="IT904_000072"
     CDS             complement(76030..76848)
                     /gene="nlpA"
                     /locus_tag="IT904_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAP3189913.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            77070..77363
                     /locus_tag="IT904_000073"
     CDS             77070..77363
                     /locus_tag="IT904_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3189914.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(77404..78594)
                     /gene="nepI"
                     /locus_tag="IT904_000074"
     CDS             complement(77404..78594)
                     /gene="nepI"
                     /locus_tag="IT904_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAP3189915.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(78805..79257)
                     /locus_tag="IT904_000075"
     CDS             complement(78805..79257)
                     /locus_tag="IT904_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAP3189916.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(79310..80644)
                     /locus_tag="IT904_000076"
     CDS             complement(79310..80644)
                     /locus_tag="IT904_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP3189917.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAIVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            80819..82585
                     /gene="adeD"
                     /locus_tag="IT904_000077"
     CDS             80819..82585
                     /gene="adeD"
                     /locus_tag="IT904_000077"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP3189918.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSVSDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(82630..84021)
                     /gene="uhpT"
                     /locus_tag="IT904_000078"
     CDS             complement(82630..84021)
                     /gene="uhpT"
                     /locus_tag="IT904_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAP3189919.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(84159..85478)
                     /gene="uhpC"
                     /locus_tag="IT904_000079"
     CDS             complement(84159..85478)
                     /gene="uhpC"
                     /locus_tag="IT904_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAP3189920.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(85488..86990)
                     /gene="uhpB"
                     /locus_tag="IT904_000080"
     CDS             complement(85488..86990)
                     /gene="uhpB"
                     /locus_tag="IT904_000080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAP3189921.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(86990..87580)
                     /gene="uhpA"
                     /locus_tag="IT904_000081"
     CDS             complement(86990..87580)
                     /gene="uhpA"
                     /locus_tag="IT904_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAP3189922.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(87655..87945)
                     /gene="ilvN"
                     /locus_tag="IT904_000082"
     CDS             complement(87655..87945)
                     /gene="ilvN"
                     /locus_tag="IT904_000082"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP3189923.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(87949..89637)
                     /gene="ilvB"
                     /locus_tag="IT904_000083"
     CDS             complement(87949..89637)
                     /gene="ilvB"
                     /locus_tag="IT904_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAP3189924.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(89743..89841)
                     /gene="ivbL"
                     /locus_tag="IT904_000084"
     CDS             complement(89743..89841)
                     /gene="ivbL"
                     /locus_tag="IT904_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAP3189925.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            90406..90495
                     /gene="tisB"
                     /locus_tag="IT904_000085"
     CDS             90406..90495
                     /gene="tisB"
                     /locus_tag="IT904_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAP3189926.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            90775..>91930
                     /gene="emrD"
                     /locus_tag="IT904_000086"
     CDS             90775..>91930
                     /gene="emrD"
                     /locus_tag="IT904_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAP3189927.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLAT"
BASE COUNT        23746 a        23329 c        22447 g        22408 t
ORIGIN      
        1 ctgtattata accgccaccg ctattatgac ccgctgcggg ggaggtatat cactcaggat
       61 ccgattgggc tgaagggggg atggaatttt tatcagtatc cgttgaatcc agttacgaat
      121 acagatcctc tggggttaga agtttttcct agaccattcc ccttgccaat tccatggccc
      181 aaaagccctg cacagcagca agcagatgat aatgctgcaa aagcattgac aaaatggtgg
      241 aacgatacag catcacaaag aatatttgac tctctaatat tgaataatcc gggactagca
      301 ttagatataa caatgatagc ttctcgtgga aatgttgcag acacagggat aactgatcgt
      361 gtcaatgaca taataaatga cagattctgg agtgatggga aaaaacccga cagatgtgac
      421 gtacttcagg aactaattga ttgtggtgat attagtgcta aagatgcaaa aagcacacag
      481 aaagcctgga attgtcgtca ctccagacag tcaaacgata aaaaaagata gcccttgtgg
      541 aggttcctgc aatgtcaaat acataccaga aaagaaaggc aagtaaagag tatggtttat
      601 ataatcaatg taagaaacta aatgatgatg aattatttcg cttacttgat gatcacaatt
      661 ccttgaaaag gatttcatct gccagagtat tacagttaag aggtgggcaa gacgctgtta
      721 gattggcaat tgagttctgc tctgataaaa attatatccg tagagatatc ggagcattta
      781 tactcgggca aataaaaatt tgcaaaaaat gcgaagataa tgtttttaat attttgaaca
      841 atatggcatt gaatgataag agcgcttgcg ttcgagctac ggcaatcgag tcaacggctc
      901 agcgatgcaa gaaaaaccca atttattcac ctaaaatagt agaacaatct caaattactg
      961 cttttgataa atcgactaat gtcagacgtg ctacagcatt tgctatttct gttatcaatg
     1021 ataaagcaac aattccacta ttgattaatc tgttaaaaga tccaaatgga gatgtcagaa
     1081 actgggccgc atttgcaata aatatcaata aatatgataa tagtgatatt agggattgtt
     1141 ttgtggagat gcttcaggat aaaaatgagg aagtccgtat tgaagcaata atcggacttt
     1201 cctacagaaa agataaaagg gttttatctg ttttatgcga tgagttaaaa aaaaatactg
     1261 tttatgatga tatcattgaa gctgcgggtg aattaggtga taaaacgctg cttcctgttt
     1321 tagatactat gttgtacaag tttgatgaca atgaaattat aacttccgct attgataagc
     1381 tgaagcgttc atagcgtgag ttgcctatgc acagtggggg attcccgccg gcacggtgca
     1441 gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg
     1501 ctggctggca tcgtgcggcc tgaccgtgga gcagatgaaa aaccagatgg acccggtgta
     1561 cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc cgctggcgct
     1621 catcagcacg gaaggggcaa cagcgtggtg cgcagaatat gatgaatggg gcaacctgct
     1681 gagtgatgag aacccgcatc atctgcagca gctcattcgc ctgccgggtc agcagtatga
     1741 tgaggagtcc ggcctgtatt acaaccgcca ccgctattat gacccgctgc aggggcggta
     1801 tatcactcag gatccgattg ggctgaaggg gggatggaat ttttatcagt atccgttgaa
     1861 tccggtcata aatgtagatc cgcaaggttt ggttgatata aatttatacc ccgaaagtga
     1921 tcttatccat tctgtagctg atgagattaa tatcccaggc gttttcacaa tcggggggca
     1981 tggtaccccc acatctattg aatccgcaac gcgcagtatc atgacagcta aagatctagc
     2041 atatctaatt aaatttgatg ggaattataa agatgggatg acagtttggt tattttcttg
     2101 taatacaggt aaaggacaaa attcatttgc tagccaatta gctaaagagt tacatacaaa
     2161 tgtaatagga cctgacacgc tatggacgtg gtgggggcga ggaactaatg gtaagttaaa
     2221 aatggataca gtgctaacag caccaacgaa ccttaattca aataaggatc taatggctat
     2281 aacaacaaaa gaccttggta attggataac atatgggcca tctgggcacc ccatttctaa
     2341 tatgcaaggt acgccagaaa aatccagtga tataagatag gttgtagatg tatgaaagca
     2401 tacttgttac tattttttta tttttctctt attggtcaat tgcatggtgc tgatgtgaaa
     2461 ataaaagaaa acgaaagtgt gatgggatct acagcaatga cctatgattt aagtgaagaa
     2521 aagctgatga aactgaagta taaatcacaa catggtgatt cagaggcatc atttcgacta
     2581 tatcaatatt actgctttac taaaaataat attgataaac aattgcgatt cttggaaaga
     2641 tcagcatctc aggggaatgt tacagcgcag tttaattatg gggtcttttt atcagataca
     2701 aatccaacat tatcagaata ttataatttg aatagagcta tttattggat ggaattcgct
     2761 gtaaataacg gtaatattga tgcaaagagc aaacttcaag agctcaaaaa gctaaagcga
     2821 atggatagaa ggaagaataa ggagaatccg taaaagcaat agttcatcag actgccgcag
     2881 cagtagtatg atgatgaatc agggctgtac tataactatt atcggtaaca ttaaccgcag
     2941 caggggcggt ataacactca ggctccgatt gggctggaag gtaggtggaa tttttatcag
     3001 tatccgttga acccgatttc tggaatcgat ccccttggga ttagccacct gtttatacag
     3061 cataacttta ggtatgcttt cgtgtgtttc agatatacca tgtgatgata atttttatga
     3121 tgtgcttagt attccagtcg tttctggtaa taatatgcaa tgtaaaaata atccccgttg
     3181 tacacattta taaatagatg gccaatacct caaggcctat ggagttggaa tgttaatgac
     3241 ccgggagcaa gcaatagaaa acctgacggc atacgattag taccgtcagt taatacagga
     3301 acttataatc gcaatggatt ttcaatacat agttgtttaa acgcttttgg tccgtcttta
     3361 ggacctcgat tctgttccga gggttgtata actggtttat ctaatgacat gcaaaaatta
     3421 aatgagttaa tattttcaga gccagatagc acattaacag ttacggacta aaaaatatgg
     3481 caaggttatt aacaattgta tttttttctc tatttctgcg agtgcagtta ctgcaagaca
     3541 atttcagaga gagttagatt tggcagacaa agcaatatta ttggcactaa tatcagccag
     3601 tactaaagag ggacgaaaag cgtgttcact aagctatttt gcctgtaagg ccgcagaggc
     3661 tgaactcgga ctggcatata tggctgcgaa tgataacaag gaatttctca catccttatc
     3721 taatataatg aggtacaaaa tagatgctgg actttccgaa tcctatacat gctatttatt
     3781 aagtaaggga aagatcataa gaccatatct gaaaaatcta aatccactcc aacttgccgc
     3841 tgattgcatt gaaacagtaa ataaaataaa agataagaat aaaaaaatca ttgatataaa
     3901 ttcagttaat atttgtagtg atgataaaaa tataaaattg agagttaata gtactattat
     3961 ggccattgat gattctataa agtgcatcga tgaatgataa cttaatttta tttaaaaata
     4021 tattaataac tcactctcca ggagtttttt taacttagtt ttataatgaa tacgataatt
     4081 aaaagtgaag aagaaaatag ttgtatacat atattccatg accctacatt gtgatctata
     4141 gattaaatat tttttaaatg tgactgtcat ccttcagata ataaagccat catcgcaata
     4201 gttggaaaag cactcctgct cttttttgat tagagccgtc gcaggaggaa caactcatat
     4261 catcattgcg atctcgacga tgtatagtct catcctgcgg cagaacaaga cggataaaaa
     4321 atccaatgag agcacactca tccagaactt attaacataa tgaatattaa agaaaaatca
     4381 tcgcagctaa tgaagtaata tgcaaaaata attagtcctt acaaggagca aagttccata
     4441 atctcccccc tccccctcaa tgatccaaat aaagataatg catccagctg gtcattctta
     4501 gcagcacttt ccgcatcacc gaaacatggc tgaaatggtc ggagtaaacc gccacctggg
     4561 cgtagatgcc gtcgggtaag gcatcgatat catcgttagg gtccagttca atggttccca
     4621 gcacaccgtc cgtgccgggc acgaccgtta atgattgcaa taccccctgc gcctgataag
     4681 aaccgcctgg cacgacaggt aaaatactgg tcagtttgcc gtggaacacc tgcccaggta
     4741 gcgcgttaaa caccacttct gcatcatcac caggtttcag acgtaacagc gagttttgcc
     4801 gaaattgggc gacaatttgc cgtttttgct cggggatgaa aaccatcacc ggacgcaacg
     4861 gcaaggcagc tgcgtatgtg cctgggcgga tcagtacctg agtgacgtag ccattgcttg
     4921 gtgcgcgaat gacagtctgc tcaagattat attttgcttc agtaagttgc gctcttaagc
     4981 tcacaatctg cgattgctcg ccgttaacca tactgtcgag ctggctctgg atctgcgcct
     5041 gctccgccac cgagcctttc accagcgcat cctgcgcgag gaaattttgc cgcgcatcgt
     5101 cgatgtcacg ttccgagaac ggattcaccg ccgcctggct gcctttcaga taacgttgat
     5161 aatttttaaa cagacggtcg cgctccgctg aaacctgggt ggtgttggcc tgcgcttctg
     5221 tgagctgcgc gcgcagagtc tttatattat gcgtcgccgt catcaggtca gcctgaagcc
     5281 tgtcaactcg cgcctggtaa cgaaccgggt cgagcttaaa aagcacctcg cccttttgaa
     5341 taagctgatt attcttgtca gtgacttcag taacaattcc cgtcacctgt ggcgtgatgg
     5401 ggatcgctat cactgccttt tgcgcggtaa aagtgtaagg gtggttgtag ttcatcaaca
     5461 aaatcaaacc actcaccaga aacacgccac ccagcgccgc cgtcgccagc gtccactgat
     5521 tcaccggaat acgaaagatt ttaaagaccg cccacgccag cgccacgtaa gttaaaacaa
     5581 tcaatagatc cataattaga tctccgggaa cgtggaatag tcagtctttt tctcggcggc
     5641 gagttgatgc tccagccggg aaattcggtc agaaagtgcg gcgatttccg ggtcagtcgc
     5701 tttctcctgc gacgcaacat gtgactgcat gccccacccg cgctccggtt gatagagcgt
     5761 cgcccagatc cacaggaacg gccagataac atgcagagta aacaggctca cccagcccgc
     5821 cgtatgaata gcgtcggcat ggggatggtt gcgctttttg gcaatcagat aagggatgtc
     5881 atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca ctcccaacgc
     5941 gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt ttttgggctg
     6001 gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg attctcgcca
     6061 taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac gattgtatga
     6121 cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat
     6181 tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact cacatttaaa
     6241 tgccattttt agcgaaaatc gccgccttgt tgctttttta cacaagcgtt ttgtgatgag
     6301 catcacgtca attacctctc taccccctat atttatgtga ctgatatcac acaaaaggcc
     6361 gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa ggtccggcta
     6421 cctctgccgc tacattaaca aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa
     6481 gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc
     6541 tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc atcaccgcat
     6601 tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctagtc ggaccgatga
     6661 tcacttacct cctgccgctg ctgatcggtt ataccggtgg taagctggta ggcggcgaac
     6721 gtggcggcgt agtcggtgcc atcaccacca tgggcgttat cgtcggcgca gacatgccga
     6781 tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag cacttcgacc
     6841 gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac ttctccgctg
     6901 gcatcatcgg gatgatcctc gccattctgg cattcctcgg cattggcccg attgttgaag
     6961 ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac atgctgccac
     7021 tggcatctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc attaaccacg
     7081 gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatcc atcttcttcc
     7141 tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc
     7201 gtggtagtgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg ggtggtatcc
     7261 acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctggcc gttatcctcg
     7321 gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt tctccagcat
     7381 ctccggggtc tatccttgcg gtactggcga tgacgccaaa aggtgcttac ttcgctaaca
     7441 tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa
     7501 ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg caggacatga
     7561 aagctgagtc taaaggcgca tctccgctgt ctgctggcga tgtgactaac gacctgagcc
     7621 acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttccagtgcg atgggcgcag
     7681 gcgttctgcg taagaaaatt caggatgcag gtctgtcgca gatttctgtt actaacagcg
     7741 cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac
     7801 gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc ctcgacagcg
     7861 gcctgtacac cagcctgacc gaacgtctgg ttgctgccca acgccacacg gcaaacgaag
     7921 agaaagtaaa agacagcctg aaagacagct ttgacgattc cagtgctaac ctgttcaagc
     7981 taggcgcgga gaacatcttc ctcgatcgca aagcggcaac caaagaagaa gcgattcgtt
     8041 ttgctggcga gcagctggtg aaaggcggtt acgttgagcc ggaatacgtt caggcgatgc
     8101 tggatcgtga aaaactgacc ccgacttatc tgggtgagtc tatcgcggtg ccacacggta
     8161 cggttgaagc gaaagatcgc gtactgaaaa cgggcgtcgt gttctgccag tacccggaag
     8221 gcgtgcgctt cggtgaagaa gaagatgaca ttgcccgtct ggtgattggt attgctgccc
     8281 gtaacaacga gcacattcag gttatcacca gcctgaccaa tgcactggat gatgagtccg
     8341 tcatcgagcg tctggcacac accaccagcg tggatgaagt gctggaactg ctggcaggtc
     8401 gtaagtaatc caatcccacc ctctccacat ggagaaggtg gggttaattg cctgatgcgc
     8461 tacgcttatc aggcctacag gatgcatcac aatttgttga atttgcacgt tcttgtaggc
     8521 cggataaggc gcttacgccg catccggcgc tgcccctctc ctcacggaga gggtttgggt
     8581 gagggaaaag cctcacccca gccctctcgg gtaaaaacat tgatgaaggt taatactatg
     8641 aaagcattac attttggcgc aggtaatatc ggtcgtggct ttatcggtaa actgctggca
     8701 gacgcgggta tccaactgac gtttgccgat gtcaatcagg tggtacttga tgccctgaat
     8761 gcccgtcata gctatcaggt acatgtggtt ggtgaaaccg agcaggtaga taccgtttcc
     8821 ggcgtcaatg ctgtcagcag cattggtgat gatgtcgttg atctgattgc tcaggttgat
     8881 ttagtcacta ccgccgttgg cccggttgtg ctggaacgta ttgctccggc aatcgccaaa
     8941 gggctggtga aacgtaaaga acaaggtaat gaatccccgc tgaacatcat cgcctgtgaa
     9001 aacatggtac gcggtaccac gcagctgaaa ggccatgtga tgaacgccct gccggaagac
     9061 gccaaagcgt gggtagaaga acacgttggc tttgtcgatt ccgccgttga ccgcatcgta
     9121 ccgccttcgg cttcggcaac taacgatccg ctggaagtga cggtagaaac tttcagcgaa
     9181 tggattgtcg ataaaacgca gttcaaaggc gcactgccga acatcccagg catggagtta
     9241 accgacaacc tgatggcatt tgtcgaacgt aaactcttca ccctgaacac gggtcatgct
     9301 ataaccgcgt acctcggaaa actggccggt catcagacca ttcgtgacgc gattctcgac
     9361 gagaaaatcc gcgcggtggt aaaaggtgcg atggaagaaa gtggtgcagt attgatcaag
     9421 cgctacggct ttgacgctga caagcatgcg gcgtacatcc agaaaatcct cggtcgtttt
     9481 gagaacccgt atctgaaaga tgatgttgag cgcgtaggcc gtcagccgct gcgtaaactg
     9541 agtgctggcg accgtctgat caagccactg ctcggtacgc tggaatatgg tctgccacat
     9601 aaaaacctga ttgaaggtat tgccgctgca atgcacttcc gcagtgaaga tgatccgcag
     9661 gctcaggaac tggcagcact gatcgctgac aaaggtccgc aggcggcgct ggcacagatt
     9721 tctggtcttg atgccaacag cgaggttgta tccgaggcgg taaccgctta taaagcaatg
     9781 caataatggt ggaccaggcg caggacaccc tgcgcccgaa taacagattg tcagatatgc
     9841 aggcaacaat ggaacaaacc caggcctttg aaaaccgtgt gcttgagcgt ctgaatgctg
     9901 gcaaaaccgt gcgaagcttt ctgatcaccg ccgtcgagct cctgaccgag gcggtaaatc
     9961 ttctggtgct tcaggtattc cgcaaagacg attacgcggt gaagtatgct gtagaaccgt
    10021 tactcgacgg cgatggtccg ctgggcgatc tttctgtgcg tttaaaactc atttacgggt
    10081 tgggcgtcat taaccgccag gaatacgaag atgcggaact gctgatggca ttgcgtgaag
    10141 agctaaatca cgacggcaac gagtacgcct ttaccgacga cgaaatcctt ggaccctttg
    10201 gtgaactgca ctgcgtggcg gcgttaccac cgccgccaca gtttgaacca gcagactcca
    10261 gtttgtatgc aatgcaaatt cagcgctatc aacaggctgt gcgatcaaca atggtccttt
    10321 cactgactga gctgatttcc aaaatcagct taaaaaaagc ctttcaaaag taagcaacgt
    10381 ctgcttactg cccctctacc tgcttcggcc gataaagccg acgataatac tccagacgtt
    10441 gtagatataa cggcacgctt tcatcgggta ttccggacgg aatcgcgtta cggggaggaa
    10501 gttttttcag atactcccgg aacgcctggc ttgatgccat gaaatctacg gctttatcga
    10561 taagaagcgg aacgttttca cctaatttgc ccatgatgat atctccgtat taccccgcgc
    10621 cgggaatgcg cggccgccaa ttttagttta ggttcccggt aaagatgaca ttaaggaaaa
    10681 cgtgctgaat cctcaataat catccgctgc tgtttattat cttttttcta tagatttctt
    10741 aattaatcaa cgaattgcgt agtttttgag catgttaatg attacgttgc agaactatta
    10801 caatgtcatc agttgtgaac aaagcacttg gtcgcgcata ctaggggcta taaatttatc
    10861 tttatcagaa gccatcacat gaaagaagtc gaaaaaaacg aaatcaaacg tctcagcgat
    10921 cgcctggacg ccatccgcca ccagcaggcc gatctgtcgc tggttgaagc cgcagacaaa
    10981 tatgccgagc tggaaaaaga gaaagccacg ctggaagcag aaattgctcg cctgcgtgag
    11041 gttcatagcc aaaaactgag taaagaagca caaaaactga tgaagatgcc gtttcagcgc
    11101 gcaattacca aaaaagagca ggctgatatg ggcaagctga agaaaagtgt tcgcggactg
    11161 gtggttgtgc acccaatgac cgcactgggc cgtgaaatgg gcctggaaga gatgaccggg
    11221 ttttcaaaga ccgcgtttta agcccctcag acgactggta cgatcgtatc aacccgcatc
    11281 tgacttgcgt tagctggtca atgcggggac tctcctgtct actcgcctaa tgaccaaaca
    11341 attcactccc cttacgtcga tttaccgctg gttgatattc tgaaaatgca tctttaccga
    11401 aataaccacc tataaacaat aaaaatatat agaagatatc atttattaat taacaagcag
    11461 ttacgtctct ttttttcgga tttaattgtg aaattttaat catcatattc atacaatcaa
    11521 ttatctgtaa aatttttccc atacatggtt tctttcatat ttgacattta ttaataatca
    11581 tcattagaat ccacgaaaaa tattatattc atacaaacac agacatcttt tgaaactaat
    11641 gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc actgtcatta
    11701 atgtaaagca acatagatca ccaataatac aatggacaac tctaccggca ttgatcgctg
    11761 gtcggaggca ataaggattt tcacatgaac ttgaagaaga tttttttcag tgctgtcact
    11821 gtttcagttc tctgcgcatt aacgggatgt gactacattc aagaaggcaa gccagaaagt
    11881 agtttactga aacaagaaga agaacataac aataaaattg tgctacttga aaaacaacaa
    11941 gcacagctaa agagtcaact cgaaactatt caaaaacaac aaactggcat aataaacagc
    12001 acaaagactt taactcacgt aattaaatcc gttaaagacc aacaaaatac ttttattttt
    12061 accgagttta acccagcaaa aacgaagtat ttcatcctga acaacggttc ggtggcttta
    12121 gcgggccgag tgttatccat tgacgccaca gagaatggca gtgttattca tatttcactg
    12181 gtcaacttat taagtatacc aatctcaaac attggcttta atgcgacatg gggtggtgaa
    12241 aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa cacatcaatg
    12301 acatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac actgaagggt
    12361 gtatcgccaa ataacctggg atatctgaag ttagccatca atatggaaaa tattcagttt
    12421 gacaatcttc ccgctgctga taatcggcag aaaagaagca aaaaataaat gcaattagta
    12481 cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag ttatctggaa
    12541 ccctgccaca gggaattata ctgttaccag cgaaacggca aaaagccgtg gcaagaaatc
    12601 tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt gggttgttgt catcgtttgg
    12661 ggcgctggct gataattacg acggtcaggg cgttgattac ggcgatggct cagctagtga
    12721 cggctgggtt gctatcggta aaggtgcaaa agcaaatatt tttttaaaca acgctggtgc
    12781 cagtaccgct ttaggttatg acgctatagc tgaaggccaa tatagctctg ccatcggctc
    12841 aaaaacccat gctattggtg gtgcatcaat ggcctttggg gttagtgcaa tctctgaagg
    12901 tgacaggagt atcgcgctgg gtgcatcgtc gtattcattc ggccaatact caatggccct
    12961 tggtcgttat tccaaagcgc tgggtagatt gtctatagct atgggggata gctccaaagc
    13021 ggatggagca aacgccattg cgctgggaaa tgccgctaag gcggctggta ttatgagcat
    13081 cggtctcggt gataatgcca atgcgtcaca agattatgct atggcgctgg gagcagaaag
    13141 cgaagccgct gaaaatgcga ccgctatcgg caataaagcg catgcaaaag gagtgaatag
    13201 catcgcgttg ggtaatggca gccaggctct ggcagatagt gcaattgcta ttggccaggg
    13261 caacaaggct aacggcgctg atgctatcgc tctgggtaat ggtagccagt cgagtggctt
    13321 aaacgctatt gccttaggta aggccagtgt tgtaactggc gataatagtc ttgcgtttgg
    13381 gagcaatacc aatgccaacg gtattaactc tgtcgcgctg ggcgcaggtt ctattgcgga
    13441 tcaagacgat agcgtttctg tcggcagtga ctcattacaa cgcaagatcg ttaatgtaaa
    13501 aaatggcacg atcaaggctg atagccacga cgccattaat ggctcacagc tttatgccat
    13561 cagcgactcg gtggcaaaac ggcttggcgg gggttcatcg gtaaacgttg acgacggtac
    13621 tgttaaagca ccaacctaca atttaaaaaa tggtaacaaa aataacgttg gcgatgcgct
    13681 cactgtactc gatcaattca ccctgcaatg ggatcaaaac agagacaaat acagcgccgc
    13741 tcatggtagc tcaactgcca gcgtaatcac cgatgtggcg gatggcgcag tttcagattc
    13801 cagtaaagac gcggttaacg gttcacaact gaaagccacc aatgatgatg ttgagaccaa
    13861 taccaccaat atcgctacca ataccggaaa tattgcaact aacactgcaa atattgctac
    13921 caacaccacc aatatcacta atttgacaga tactgttggc gaccttaaaa atgatgccct
    13981 gctctggaat ggcactgcat tcaacgccgc tcatggtacg gaaaccacca gcaccatcac
    14041 taatgtgaaa gctggcaccc tttcggatga cagtactgac gcggttaatg gctcgcaact
    14101 gaaagctact aacgataacg tggcaaccaa caccaccaat atcgccagca atacggctaa
    14161 cattgcgacc aacaccgcca atattaatac tctcaatacg tcgatcgata cccttgagca
    14221 ggatgcaata ctctggaacg gaacagcgta tagcgccgcg cacggtacag aaacagccag
    14281 cactattacc aacgttaaag caggtacttt atctgaaaat agtacggacg cagttaacgg
    14341 tgcgcagctg aacgcgacta acgcgaatgt ggcgaccaac accaccaata ttgctactaa
    14401 caccgccagc attaatactc tgaatacatc cattgacgct ctggaacagg atgcgctgct
    14461 ttgggatggc actgcattca gtgcagcgca tggtgcaaac aaagatgcca gcaaaatcac
    14521 caatgtcctg gcagggactg tatcatccgc cagtactgat gccattaatg gcagccagct
    14581 tcatgggttg agcagttcga tcgccaccta tcttggtggt ggtgccactg tgagcgattc
    14641 tggcgtattt agcggcccta cctataacat tgatggtaat gattacacca atgttggtgc
    14701 tgcacttgac gccattaaca cctcacttag cgactcactc ggcgatgccc tcctttggga
    14761 tagcacgact ggcgcattta gtgccaaaca cggttctacc gccagcgtaa tcactaacgt
    14821 cgcagacggt gcagtctctg actctagctc tgatgctgtg aatggttcac aactgtacga
    14881 tgtaagcaac tctgttgtcg atgttctggg tggtggtgct ggcgtgaata cggatggcag
    14941 catcagtgca ccaacgtaca ccattgctaa cactgattac gataatgtcg gcgatgccct
    15001 aaacgcgctt gataccactc ttgacgatgc gatgctatgg gatgctaccg caggtgaaaa
    15061 tggtgccttc agtgccagcc acgatggcag tgccagcaaa ataactaacg tcgcggcagg
    15121 aacaatttct gacaccagca ccgatgcggt taatggggct caactccacg gcgttagcag
    15181 ttccgtcgct gaggctcttg gtggtggtgc ggcggtgaat tctgacggca gcatcagcgc
    15241 accgacttac accattgctg ataccgacta taccaacgtc ggcgatgcga tgaatgcaat
    15301 tgactcaacc ctggataacg ccttgctttg ggacgctacc gcaggtgaaa acggtgcgtt
    15361 taacgccagc catgatggca aagccagtgt catcaccaac gtagccaatg gtcagattaa
    15421 cgaaaccagc accgacgcgg ttaacggttc ccagttaaat gccacgaata tgttgatcca
    15481 gaatattgcc ggtgatacca gcgaaagcta tatcacagag aacggtgaag gtatcaacta
    15541 tgtgcgtact aacgacagcg gcttagtctt tgaggatgcc agtgctacgg gtgtgggcgc
    15601 tacagctgta ggttataact ccgtcgcatc aggcgatagc agtgtggcaa ttggacagaa
    15661 cagcagcagt actattgaat ctggaattgc tctgggcagc agttctgtgt ctaaccgtgt
    15721 catccttcag ggctcccgtg ataccagcgt aaccgaagat ggcgtagtca ttggttataa
    15781 caccagtgat ggcgaactgc ttggcgcgtt gtcaattggt gatgacggta aatatcgtca
    15841 aatcatcaac gtcgccgatg gttccgaagc ccatgacgcc gttacggttc gccagttgca
    15901 gaatgcgatt ggtgctgtag caacgacacc gtccaaatac ttccatgcta attcaacgga
    15961 agaagattca ctggctgtcg gtgaagactc actggcaatg ggtgcgaaaa ccattgttaa
    16021 tggtgatgcc gggatcggta ttggcctgaa cactctggtg ttaactgatg caatcaacgg
    16081 tattgctatc ggtagcaacg cgagtgcaaa tcatgcaaac agtattgcga tgggtagtgg
    16141 ttcccagacc acccgtggtg cgcagactga ctacaccgcc tacaacatgg acgcgccgca
    16201 gaattctgtc ggtgaattct ctgtcggcag cgaagacggt caacgtcaga tcaccaacgt
    16261 cgcggctggt tcagcggata ccgatgcggt taacgtaggt cagttgaaag tcactgatga
    16321 gcgcgtagcg caaaataccc agagcattac taacctgaac aatcaggtca ctaatctgga
    16381 tactcgcgtt actaatatcg aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa
    16441 gtacttcaag accaacaccg atggcgtaga tgccaacgcc cagggtaaag atagcgttgc
    16501 tattggttct ggttccattg ctgccgctga caacagcgtc gcactgggta ccggttccgt
    16561 tgcagatgaa gaaaatacaa tctctgtagg ttcttccact aaccaacgcc gtattactaa
    16621 cgttgccgca ggtaaaaatg ctaccgatgc tgttaacgtt gcgcagttga agtcttctga
    16681 ggcgggcggc gtgcgttacg acaccaaagc tgatggttct atcgactata gcaatatcac
    16741 cctcggtggc ggcaacggtg gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa
    16801 caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg caggaaacga agcaatacac
    16861 cgatcagcgg atggttgaga tggataacaa actgtctaaa accgaaagca agttgagtgg
    16921 tggtatcgct tctgcaatgg caatgaccgg tctgccgcag gcttatacac cgggtgccag
    16981 catggcttct attggtggcg gtacttacaa cggtgaatcg gcagttgctt taggtgtatc
    17041 gatggtgagc gccaatggtc gttgggtcta caaattacaa ggtagtacca atagccaggg
    17101 tgaatactcc gccgcactcg gtgccggtat tcagtggtaa tgatccatta agttagtgtg
    17161 actaagggca aacgccaaat tgcctgatgc gctacgcgca tcaggcaaga taaggcgctc
    17221 tggatcaaca acctaagggc aattctctga tgaggattgc ccttttcttt accagacatc
    17281 tccccccaca agaattggcc ctaccacttc ttcgcttatc tgacctctgg ttcacaattt
    17341 cccaattaaa actcacatca atgttgccaa tacataacat ttagttaacc attcattgtc
    17401 attatcccta cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg
    17461 agcaacagac tcattacacg atgtgcgtgg actccaggag acctgcaatg aatctctggc
    17521 aacaaaacta cgatcccgcc gggaatatct ggctttccag tctgatagca tcgcttccca
    17581 tcctgttttt cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcgt
    17641 ggacggtggc aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg
    17701 cgctggcctc ggtggtttat ggtttcttct acgggttgtg gcccatcgcg tggatcatta
    17761 ttgcagcggt gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcattcgct
    17821 cgtctattct ttcgataacc cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt
    17881 tcggcgcgtt ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat
    17941 tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg gctgtgcctg attgttaaca
    18001 ccgcgccagt ggcatttggt gcgatgggca ttccaatcct ggttgccgga caggtaacag
    18061 gtatcgacag ctttgagatt ggtcagatgg tggggcggca gctaccgttt atgaccatta
    18121 tcgtgctgtt ctggatcatg gcgattatgg acggctggcg cggtatcaaa gagacgtggc
    18181 ctgcggtcgt ggttgcgggc ggctcgtttg ccatcgctca gtaccttagc tctaacttca
    18241 ttgggccgga gctgccggac attatctctt cgctggtatc actgctctgc ctgacgctgt
    18301 tcctcaaacg ctggcagcca gtgcgtgtat tccgttttgg tgatttgggg gcgtcacagg
    18361 ttgatatgac gctggcccac accggttaca ctgcgggtca ggtgttacgt gcctggacac
    18421 cgttcctgtt cctgacagct acagtaacac tgtggagtat cccgccgttt aaagccctgt
    18481 tcgcatcggg tggcgcgctg tatgagtggg tgatcaatat tccggtgccg tacctcgata
    18541 aactggttgc ccgtatgccg ccagtggtca gcgaggctac agcctatgcc gccgtgttta
    18601 agtttgactg gttctctgcc accggcaccg ccattctgtt tgctgcactg ctctcgattg
    18661 tctggctgaa gatgaaaccg tctgacgcta tcagcacctt cggcagcacg ctgaaagaac
    18721 tggctctgcc catctactcc atcggtatgg tgctggcatt cgcctttatt tcgaactatt
    18781 ccggactgtc atcaacactg gcgctggcac tggcgcacac cggtcatgca ttcaccttct
    18841 tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg gtcggatacc tcatctaacg
    18901 ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat tggcgtctct gatctgttgc
    18961 tggttgccgc caataccacc ggtggcgtca ccggtaagat gatctccccg caatctatcg
    19021 ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg
    19081 tcaaacacag cctgatcttc acctgtatag tgggcgtgat caccacgctt caggcttatg
    19141 tcttaacgtg gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc
    19201 cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc
    19261 tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat tcactgcgcg aggcgctggc
    19321 aaaactggtg agtgaaggcg tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg
    19381 gcgtcatgac acatggtcgg agcaaaacat cgtccagccg ctaaaaacac tgatggccga
    19441 tgatccggat tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc
    19501 atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc tttgctttga
    19561 agcaacgcta agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc
    19621 gattgccgaa gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt
    19681 cctgcaatcc tcagtgaagc atagccgtca gcggatgtat ctggtgccac cggttttttc
    19741 acaactgacc gaacaacatc aggctgtcat tgacgccatt tttgccggtg atgctgacgg
    19801 ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac accaccatga aacgattcga
    19861 tgaagatcag gctcgccacg cacggattac ccgcctgccc ggtgagcata atgagcattc
    19921 gagggagaaa aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc
    19981 attctgccgc cgttcctgtt ccactatatg gatggtggtg catattctga atacacgctg
    20041 cgccgcaacg tggaagattt gtcagaagtg gcgctgcgcc agcgtattct gaaaaacatg
    20101 tccgacttaa gcctggaaac gacgctgttt aatgagaaat tgtcgatgcc ggtggcactg
    20161 gctccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg
    20221 gcggacgcgc atggtattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa
    20281 gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc
    20341 tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc
    20401 gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc attcaggtat gagcggcccg
    20461 aacgcggcaa tgcgccgcta cttgcaagcg gtgacacatc cgcaatgggc gtgggatgtg
    20521 ggcctgaacg gtcgtccaca tgatttaggt aatatctcag cttatctcgg caaaccgacc
    20581 ggactggaag attacatcgg ctggctgggg aataacttcg atccgtccat ctcatggaaa
    20641 gaccttgaat ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat
    20701 ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac
    20761 ggtggccgcc agctggacgg tgtactctct tccgcccgtg cactgcctgc tattgcagat
    20821 gcggtgaaag gtgatatagc cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc
    20881 gtgcgtatga ttgcgctcgg tgccgacacc gtactgctgg gtcgtgcttt cttgtatgcg
    20941 ctggcaacag cgggccaggc gggtgtagct aacctgctaa atctgatcga aaaagagatg
    21001 aaagtggcga tgacgctgac tggcgcgaaa tcgatcagcg aaattacgca agattcgctg
    21061 gtgcaggggc tgggtaaaga gttgcctgcg gcactggccc cgatggcgaa agggaatgcg
    21121 gcatagtcgt ttgcgcccct ccccctaacc ctctccctca gggagctgtc tcttgatcag
    21181 atctcctgat caagagacag ggggaggccg gggtgagggg aaaaggccgc actgctcacc
    21241 aattttctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc gtactttacg
    21301 aaccagaaat tccgccaaat actggcaaca tcatccgtct ttgcgctaat accggctttc
    21361 gtctgcatat catcgaaccg atgggatttg cctgggacga taagcgcctg cgccgcgcgg
    21421 ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactttcgc gcgttcctcg
    21481 aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct gctcacagcg
    21541 ccgtaagcta tcaggatggc gattatctga tgttcggccc ggaaacacgc ggcctgccag
    21601 cgagcattct tgatgccctg cccgctgaac aaaaaattcg cattccgatg gtgccggaca
    21661 gccgcagcat gaacctgtcc aatgcggtgt cagttgtggt gtatgaagcc tggcggcagc
    21721 tgggatatcc gggagcgttg ttgagagatt agttactgta tgccggatag atgattacat
    21781 cgcatccggc acgatcacag gacattagat cccatcccca tactcaaatg tatggttaat
    21841 accgttgaaa tgctggtcca tatccattga tggcttatcg ctgtctggtt taccgacaat
    21901 acgagccgga acgccagcgg cggttgtatg cggcggcacc ggttgcagca ccacggaacc
    21961 tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg aggattttcg cgcccgcgcc
    22021 aatcatcaca ccttcacgaa ttttcgggtg acggtcacca ccagatttac ccgtaccgcc
    22081 aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt accgccgttt cacccacaac
    22141 gatgcccgtg gcgtggtcga gcataatgcc acggccaatt tttgctgccg ggtgaatatc
    22201 gacctggaac gtcacagaaa cctggttttg cagaaagatt gccaatgcgc gacgcccctg
    22261 attccacaac cagtgaccga tgcgataggc ctgcaaggca tgaaaaccct tcaggtataa
    22321 caacggggtt gagtatttat cgactgccgg gtcgcgggta cgcaccgcct gaatatcaca
    22381 ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct tcttccacca cttcacggat
    22441 agcaatagca ggcataattg gcgatgacag cttgttcgcc agcatgtagc tcagtgcact
    22501 tccaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca ttggctcaca
    22561 gtccgccagc gttctggctt cggctttaat attgttccag acaatttcca gttcttcaca
    22621 cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg ggccgggtca
    22681 tagcggtaac aaaggttccc tggggttagt ggctgctgcg ctcgtccttg cgtgcacgac
    22741 ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca atataatact tgataaattt
    22801 cctcggttat tggcatttct acgccgaagc gatgcgccag ttcgcggact tctttcgtat
    22861 tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc gctttgtaca tccatgccct
    22921 gaccgagcat catgccaaaa cggcggttac gcgactggtt gtcggtacag gtaagcacca
    22981 gatcccccag ccccgccatg cccataaagg tggcaggatc ggcacccagc gccgcgccaa
    23041 gacgcgacat ttcagccagc ccacgggtga tcagcgccgt acgcgcattc gcaccaaaac
    23101 cgataccgtc ggacatcccc gcaccaatgg caatgacgtt tttcaccgcg ccgccaagct
    23161 gcacgccaat gaaatccggg ttactgtaaa cgcggaaact tttgccgcag tgcagcagct
    23221 gctgaagatc atcagcaaag gtctgatcgg tcgaggccag cgaaatagct gtcggtaaac
    23281 ctgccgccag ttctttcgca aacgttgggc cagagataac cgccagcgga atttgatcgc
    23341 ctaacgcctc gcgggccacg tcctgtaaca gacgcccggt ttccgcttcc agccctttgg
    23401 tcgcccacac cagacgcgca tcaggacgca tcagcggttt aatctggcgc agcacttcac
    23461 caaagacatg gctgggtacg acgacgagaa tattacggct ggctgccagc gcagtggcga
    23521 gatcgctttc aagatggagc gtatcgggaa aaggcacatc ggggagaaac gcggcgttac
    23581 agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg gccccagagg acaacctcgt
    23641 ggccatttct tgccagggta atggcaagag cggtgccgta cgagccggca ccgatcacag
    23701 tcattgaagc attacgttgg ttcatcaggc atcctgatgt tcttcagtac cttcgccagc
    23761 ctgctgctgc aaatagttca tgaacagcgc atcgaagtta accggcgcaa ggttcagttg
    23821 cgggaatgta ccgcgggata ccatgctggt gatgcactca cgagcatacg ggaacagaat
    23881 gttcgggcag tatgcgccca ggcaatgtgc catctgggtg ccttcgatac ccgcgatgga
    23941 gaaaataccg ccctgctgaa cttcacacag gaacgcggtt tcttcgccca gggaggccgt
    24001 tacggtaaca cgcagtacca cttcgtatac gtcatctgcc agttgggaag atgccgtatc
    24061 cagatcaagt ttaacttctg gttgccaatc tttctggaaa acgtgcggcg cgttcggcgc
    24121 ttcgaaagag atatccttgg tataaatacg ttggatctgg aaagtcattt cagtgttgtt
    24181 ttgttctgac atgtgtagaa aacccttaag tgttgtcctt aaatactgcg caatgtaatg
    24241 ccaacgttca cacgttattt cagcagggga tccagtccac cacgtgcatc caatgcatac
    24301 aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa tctggggaac cgtggtgcga
    24361 ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc catcgatcgg caactcctga
    24421 aaactcacgc ccttgctgct cagcagtgct tttgcacgat ggcaatacgg gcaggtttct
    24481 ttggtataga tttcaacatt ggccataatt tagttcctgt ttttttaccc tgaaatttca
    24541 tgttgcaggg cggctgcaaa atggagtgtc cccagaagct tataacaata agtcgctggg
    24601 ggcctaattg cagctaacgg cctgcatcat gaaagacgac aggtaaatta tttgccgcgc
    24661 accagaggca agttttcgcc agcccagcca gcaacgccct ctttcagcac gaatacttgc
    24721 gcaaaaccag ctttcgtcag cgcgtttgca ggctcctggc actgcatgcc agaaccgtct
    24781 accacgataa ccggtttgtc tttgtgcttc tcaagctcac caacattgtt ggctttgatt
    24841 tcgctcggca acagattgat agaacctgcg atatggcctt tacggaagtc atcacgctga
    24901 cgtaaatcca caaccacagc gtcttctttg ttgatcagac gcgtagcttc accacgagta
    24961 atcaccttca ctttcgaggt caggctctta aacgtagtca caagaaccgc caccagtaac
    25021 gcgatccagg cgatactcag tatgggatga cggccaacaa attgcataat ttcttgcatg
    25081 gggggtaaca actcccgacg tagtgattaa aaaaaccagg aaaggagtat acctgcgcgg
    25141 tgcggcaaat acagccagcg tgttaactgg aatgcaattt tgcggggcac gacgaaaaaa
    25201 aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat attttgatct tcagaggcta
    25261 ttttatcgat tcagctgtag taaaattacg caaattttga ctcttgagta tgaggttgtc
    25321 gcaatgtcgg tttctaaaaa acctatggta ctggtgattc tggatggcta tggctatcgt
    25381 gaagaacagc aggataacgc catttttagt gctaaaaccc cggtaatgga tgcactgtgg
    25441 gccaatcgtc cgcataccct gatcgacgct tccggtctgg aagtcggtct gcctgaccgt
    25501 cagatgggta actccgaagt aggccacgtt aacctgggtg ccggccgcat cgtgtatcag
    25561 gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt tctttgctaa tccagtgctg
    25621 actggcgcgg tagataaagc gaaaaacgca ggtaaagcag tacacattat gggtctgctc
    25681 tccgcaggcg gcgtacacag ccacgaagat cacatcatgg cgatggtaga actggcagct
    25741 gaacgcggcg cagaaaaaat ctacctgcac gcattccttg acggtcgcga caccccgccg
    25801 cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat ttgctgcgct gggtaaaggc
    25861 cgcgtagcgt ccatcattgg tcgttactac gcgatggacc gcgataaccg ttgggatcgc
    25921 gttgaaaaag cctatgacct gctgactctg gcgcagggtg agttccaggc cgatactgcc
    25981 gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg acgagttcgt gaaagcgacc
    26041 gttattcgtg cagaaggtca gccagatgcg gcaatggaag acggcgatgc gctgattttc
    26101 atgaacttcc gtgctgaccg cgcgcgcgaa atcactcgtg cattcgtaaa cgcagatttc
    26161 gacggcttcg cgcgtaagaa agtggttaac gtcgatttcg tgatgctgac cgaatacgcc
    26221 gctgacatca aaactgcggt tgcttaccca cccgcttccc tggttaacac cttcggcgag
    26281 tggatggcga aaaacgacaa aactcagttg cgtatttccg aaaccgaaaa atatgcccac
    26341 gttactttct tcttcaacgg tggcgtagaa gagtcgttca aaggcgaaga tcgcattctg
    26401 atcaactcac cgaaagtggc tacctacgat ctgcaaccgg aaatgagctc cgcagagctg
    26461 accgaaaaac tggttgcggc aatcaagagc ggcaaatacg acaccattat ctgtaactat
    26521 ccgaacggcg acatggtagg tcacaccggg gtgatggaag cggcggttaa agcagttgaa
    26581 gcgctggatc actgcgtgga agaagtcgcg aaagcggttg agtccgtggg tggacaactg
    26641 ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg atccggcaac gggtcaggca
    26701 cacacggcac acaccaacct gccagttccg ctgatttacg ttggtgataa gaacgtgaaa
    26761 gcggttgcag gcggcaaact ttctgacatc gcgccgacca tgttgtcgct gatgggtatg
    26821 gaaatcccgc aagagatgac tggtaagccg ctgttcatcg tggaataatc cctccccatg
    26881 aggggaaagg cgattaatac catgacacgg gccgtgaaac cgcgcaggtt tgcaatcagg
    26941 cccatcatct acgccagcgt tcttagcgct ggcgtattgt tgtgcgcctt ttccgcccac
    27001 gcggatgagc gtgaccaact caaatctatt caggctgaca tcgccgcaaa agagcgcgcg
    27061 gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac aattgaaaaa gcaggaagaa
    27121 gcgatctctg aagccactcg caaactgcgc gaaacgcaaa acacgctcaa tcaactcaat
    27181 aaacagattg atgagatgaa cgcgtcgatt gccaaactgg agcagcaaaa agccgcccag
    27241 gagcgcagcc tcgccgcaca actggatgcc gcattccgtc agggcgagca taccggtatt
    27301 cagctaattc tcagcggtga agaaagccag cgtggacagc gtttacaggc ttatttcggc
    27361 tatctcaacc aggcgcgaca agaaaccatt gcccagttga agcaaacgcg tgaagaagtc
    27421 gccatgcagc gtgctgaact ggaagagaaa caaagcgagc aacaaacgct gttatatgaa
    27481 cagcgcgccc aacaggcgaa actgactcag gctctgaacg agcgtaaaaa gacgctggcg
    27541 gggctggaat cttccatcca gcaaggtcag cagcagctaa gtgaactgcg tgccaacgaa
    27601 tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg cgaaagcgcg tgctgaacga
    27661 gaagcgcgtg aggcccaggc ggttcgcgac cgccagaaag aagcgacgcg caaaggcacc
    27721 acctataaac cgaccgaaag cgaaaaatcg ctgatgtccc gaactggtgg cctgggcgcg
    27781 ccgcgtggtc aggcattctg gccggttcgc gggccgacgc tgcatcgcta tggcgaacag
    27841 ctacagggtg aattacgctg gaaagggatg gtgattggtg cctctgaagg tactgaagtt
    27901 aaagcgattg ccgacggtcg ggtgattctg gctgactggc tgcaaggcta cggtctggtg
    27961 gtggtggttg agcatggtaa aggcgacatg agtctttacg gctataatca gagcgcactg
    28021 gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg cactggtggg cagcagtggc
    28081 ggtcagggtc ggccttcact ctatttcgaa attcgccgcc agggtcaggc ggtcaatcca
    28141 cagccgtggt tgggaagata agttttgttt ccatttcgtc gtaacgttct tgcatttgcc
    28201 gctctgttgg cgctctcctc ccccgtactt gctggcaaac ttgccatcgt cattgatgat
    28261 tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga tgccttccgc tatctccgtc
    28321 gctgtattac ccgattcacc gcacgccaga gagatggcga ccaaagcgca taacagcggt
    28381 cacgaagtgt tgattcatct cccgatggca ccgttgagta aacagccgct ggagaaaaat
    28441 acgctacgcc cggagatgag cagcgacgaa attgagcgca ttattcgtag tgcggtcaat
    28501 aacgtgccct atgccgtggg gatcaacaac cacatgggca gcaagatgac ctctaacctg
    28561 tttggtatgc agaaagtgat gcaggcactg gagcgttaca atctttactt cctcgacagc
    28621 gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag gtactggcgt gaaggtgatc
    28681 aaacggaagg tgttcctcga cgattcgcag aacgaagcgg acatccgtgt gcaatttaat
    28741 cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg ccattgggca tcctcaccct
    28801 tcaacggtac gcgtgctaca acagatggtt tataacctgc cgccagacat tacgctggtg
    28861 aaagccagca gcttgctgaa tgaaccgcag gttgatactt ctacaccgcc gaaaaacgct
    28921 gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc tgtgcaaacc gaagaaaccg
    28981 atagaacctg tttatgctaa tcgcttcttt gaagtattaa gcgaaagcat cagccagagc
    29041 acgctgatcg tttacttcca gcatcagtgg caaggctggg gcaaacagcc cgaagcggcg
    29101 aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat gcgatggtgc gaccgcgttt
    29161 tgtcgcgcca ttgccataag cggaaagacc acagtaacgc ctggtaaccg accttcacgc
    29221 tgcgcacatt ggtaatcagg cgcttataca taccggaagt aaatatctcg gcaatcatcc
    29281 gttggcgggt aagaatatcc ggctctttgc gcaccgcatg gcaaacacgc aatgcctcgt
    29341 aagttatttg ctgatgaaat tccggataaa tcataatttt gtcggcataa tttcgattta
    29401 atttctccag caggcgggta atcttaatat agtgacgttg ataattaagg tttttattcc
    29461 cttgtctatg caaccgactc actgacgtat tatgcagata atatttatat aatgattgct
    29521 cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt tgtccagaca atatcctgat
    29581 gatgtaatcc ggcaataaat ttaatgttat ttttaacaat aacatcacgg cgataaaccc
    29641 ccatccagac aacgtgagtc caacggcgcg aagaaagccc catccgcagc cagtccgggc
    29701 cggttaagac gccggttgag cgaaggcgat cggtggggat ggattgccag gtttctcccg
    29761 tttcacgaaa acaccagtca gcgttgcact gcgccacgtc gaggtcgtcc tctaacgcca
    29821 tggtcatcag cgtttcgtac atggtgggat agacttcatc gtcagcatcg acaaaagcga
    29881 catatttgcc cgttgccact tcaatcccac gattacgcgc caccgatgcg ccagcattcg
    29941 cttgatgcaa caaacgaacg tgcggatagt tttctgcgta atactttgct atttcaacag
    30001 aattatccgt tgaaccatcg ttaataataa tgatttccag agcagtccag gtttgcgtaa
    30061 ttaaagattc catacaagtg cggaaatcat cgcccgcatt atataacgga ataataacac
    30121 taagtttatt ggtgctgttc atcataaatt accgacgacc tctgaagtta tcctgacgtt
    30181 ttacaggctt tgtattaaga aactattaag cctgtgagat attattttta caggtgagag
    30241 agtcacttca gggtagagag ataatgagag cagctatata agagatcagc acaattacga
    30301 actctttatt acactgacag gattttacat atttaacaga ttatcctcag ataaaagggc
    30361 tggaatacca gcccttgttc gtgttaatcc cagctcagaa taactttccc ggactggccc
    30421 gaacgcatag cgtcaaagcc cttctggaaa tcatcgatag agaaacgatg ggtaatgatc
    30481 ggcgaaagat cgaggccaga ctgaatcagc gccgccatct tgtaccaggt ttcaaacatc
    30541 tcacgaccgt aaataccttt aatgaacaag cctttaaaga tcactttggt ccagtcgata
    30601 gacatatcag acggcggaat acccagcatc gcaatacggc cgccgtggtt catggtgtca
    30661 agcatggtac gaaacgctgg cggcgcaccg gacatttcca gaccgacatc aaaaccttcg
    30721 gtcatgccta actccgccat cacgtcattg agattttctt tggcgacgtt aaccgcacgg
    30781 gtgataccca ttttacgcgc cagctcaagg cggtattcgt taacatcagt gatcaccaca
    30841 ttgcgtgcac caacgtgttt cgccaccgcc gctgccataa taccaatcgg gcctgcacca
    30901 gaaaccagca catcctcgcc aaccagatcg aacgacagcg cggtatgcac ggcattaccg
    30961 aagggatcaa aaatggaagc caggtcgtcg gagatattgt cgggaatttt gaaggcgttg
    31021 aacgccggaa tcaccagata ttcggcgaag cagcccgggc ggttaacgcc gacgccgatc
    31081 gtgttgcggc acagatgggt acgaccgcca cgacagttgc gacagtgacc gcaggtgata
    31141 tggccttcgc cagaaacgcg atcgccaatc ttgaagccct tcacttcctg accaatacct
    31201 accacttcac cgacatattc atggcccacg accatcggca ccgggatggt tttttgcgac
    31261 cactcatccc agttatagat gtgaacgtca gtcccgcaga tggctgtttt acggatttta
    31321 atcagcagat cgttatgccc gagttccggt acaggaacgt cggtcatcca gatgccctct
    31381 tccgctttca gtttggataa cgctttcatc tcacatcctc aggcgataac gcccagttgt
    31441 ttaccaatac gcgtgaatgc ttctactgca cgcgtaattt gctcaggggt atgcgccgcc
    31501 gacatctggg tacgaatacg cgcctgacct ttcggaacga ccggatagaa gaaaccggtg
    31561 acgtaaatgc cctctttttg cagctcacgg gcaaatttct gcgccactac cgcatcacca
    31621 agcatgaccg gaataatggc gtgatcggct cccgccaggg taaagcccgc cgccgacatt
    31681 tgcccacgga actgacgcgc gttcgcccac agacggtcac gcaactcgct acccgcttca
    31741 accatctcca gcactttgat ggaagcggcg acaatcgctg gtgccagcga gttggagaac
    31801 aggtacggac gagaacgctg gcgcagccac tcaaccactt ctttgcgcgc cgcggtataa
    31861 ccaccagaag ccccgcccag cgctttaccg agcgtgccgg tgatgatgtc gacacgcccc
    31921 atcacatcgc agtattcatg ggaaccacga ccattttcac cgacaaaacc gaccgcgtgg
    31981 gagtcgtcta ccatcaccag ggcatcgtat ttatctgcca gatcgcaaac gcccttcaga
    32041 ttggcaatca cgccgtccat cgagaacaca ccatcggtgg caatcagcac atgacgcgca
    32101 ccggcttcac gcgcttcttt cagacgagct tcaagctcct gcatatcgtt gttggcatag
    32161 cgatagcgtt tagctttaca cagacgcaca ccatcaataa tagacgcgtg gttcagtgcg
    32221 tcggagataa tggcgtcttc cgcgcccagc agcgtttcaa acaggccacc gttagcatca
    32281 aagcaggaag agtagagaat cgcatcttcc atccccagga atgccgccag tttttgttca
    32341 agctctttat ggctgtcctg agtgccgcaa ataaaacgca ccgaagccat gccgaaaccg
    32401 tgagaatcca ttcccgcctt tgctgccgca atcagatccg gatgattcgc cagcccgaga
    32461 tagttgttgg cacaaaagtt aatgacgtgg cttccatcag ccacggtgat atctgcttgc
    32521 tgcgcagacg taataatgcg ctcttcttta aacaaccctt ccgcccgtgc ggtttccaga
    32581 tcgttggtta actgctgata aaattctcca cgcattgcga ttctccagac agggcaaatt
    32641 ccagcacata ttacccaaac ttataggtcg cgacgagata acgcgttaac acttctgcaa
    32701 aattcaggat aaataacgtc catcaatctc acttttggcg aatggctgaa gccaacgccg
    32761 tgtagtgata tgatagaagt attcgtgtct gagattgtct ctgactccat aattcgaagg
    32821 ttacagttat gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg
    32881 ccctgaatga taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca
    32941 agtttgtgaa cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga
    33001 tccagattat ggctggcgaa gagttcggcg atgtcgaagc gattttccac gaaggcgcat
    33061 gctcttccac caccgagtgg gacggcaagt atatgatgga taacaactat caatactcca
    33121 aggagctgct gcactactgc ctggagcgcg aaatcccgtt cctgtacgct tcttccgcag
    33181 ccacctacgg cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga
    33241 acgtctacgg ttactcaaaa ttcctgtttg atgaatatgt tcgccaaatc ctgcctgaag
    33301 cgaactcgca gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata
    33361 aaggcagcat ggcgagcgtc gctttccatc tcaacactca gcttaacaaa ggtgaatcac
    33421 cgaagctgtt tgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg
    33481 cagatgtaaa cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggtactg
    33541 gtcgtgcgga atccttccag gccgtagcag atgctactct ggcttatcac aagaaaggcc
    33601 agatcgaata cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg
    33661 cagatctgac aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag
    33721 gtgtaatgga atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac
    33781 tggtgatcgg cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc
    33841 tccaggcgcg ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat
    33901 tattatcgcg gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc
    33961 tggaaatcgg cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg
    34021 cctacgtctt accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc
    34081 atcgcactgg ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg
    34141 ataaagaagc ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca
    34201 ttatgcgtac cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg
    34261 aaggtgaaaa atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg
    34321 gcttttgccc gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg
    34381 agctggcaaa gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag
    34441 atcatgaagc gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc
    34501 ggaacctggc gggggaaaca cagcttgatc aagcggttat cctgattgca gcctgtaaag
    34561 ccattgtcac taacgattct ggcctaatgc acgttgcggc ggcgctcaat cgtccgctgg
    34621 ttgccctgta tggtccgagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg
    34681 tgatccgtct gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc
    34741 accagagctt gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt
    34801 tacaagagga agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg
    34861 ttctccatac gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg
    34921 actgggtggt ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag
    34981 ttattcctgt ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg
    35041 aacgcaaagc gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgatgctc
    35101 aggggctggt aaaaagcgcg gcgctggtga cgcgtctggc gcatggcgta aagcatggat
    35161 tggactggca aaccgctcgc gaaccgttag ccagcctgtt ttacaatcgt aagcatcata
    35221 ttgcaaaaca gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtctgggct
    35281 atagcaaacc gcaaacccag ggcgattatg ctattgcaca gcatttcctg acgaacctgc
    35341 ctacagatgc tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact
    35401 ggccggaaga acactggcga gaattgattg gtttactggc tgattcagga atacggatta
    35461 aacttccgtg gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg
    35521 cttatgttga agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg
    35581 ctaaatttgt agtgtcagtg gatacggggt taagccattt aacagcggca ctggatagac
    35641 ccaatatcac ggtttatgga cctaccgatc cgggattaat tggtgggtat gggaagaatc
    35701 agatggtttg tagggctccg gggaatgagt tgtctcaatt gacagcaaat gctgttaagc
    35761 ggttcattga agaaaacgct gccatgattt aaattacaac tctttaatag ataattttga
    35821 aataaaatca gcttccttgc tgattttatt tcacatattc attacttctc taataaacat
    35881 tggtccgatt gtgctttaaa ataagcacaa agcaatatta tggtgataaa cacagcagtc
    35941 ggaaccggtt tagaaaagag tattacatca cttagtccta ctaccagtag tgaaaaaagc
    36001 aagattaaaa tataaggttc tttagcagtt cttaaggcat atatgagtat tgctgaaaat
    36061 gctaaaattg ttaatacaac tccaggaata ccacgcgttg ataacgtatc tatgagatcg
    36121 ttatgcaaat gagaatctac atagggtaaa gcgccactca atctaggctc tttttcttct
    36181 agttcatgta ttttttccgc acgtttttcc agtgattgtc ctattggaga atatgttttt
    36241 aagccaactt catacattgc cagacgggca ccgactgaag ttctggtgtt atcatgcgaa
    36301 taattaacta aatcgttatt taagtcattc attctcataa gcaatgtatc tttaaaagaa
    36361 aatacacctg ctaataatgc aatcgtaata aaacaatata acttataatt aattcgctta
    36421 ttaatataac tatccattac gattaatatt aacgcaaaga atggaaacac taatatggct
    36481 gcacgcgtct cagttactat gacagcacat aagctaagca tgaagttaat tgtatataat
    36541 gttgttttat gtctaaagtc ggattttaaa atgagaattg atgccagtaa agaaggaaaa
    36601 aggatgatgt agcctgctgt tgtagcgtgg gaagctgata atgcaacacg gtcgacgttt
    36661 aaatagaaat gttgccacat ggcatgcgca aaaaaaagta actgcgttgc cagggatgcg
    36721 aacagaatta gtttcccaaa ttttattttt gttctcatct catttttagc aaaaagaact
    36781 aagaaaataa aagcgctaca caaggcaatt tttccagttt ccattgggcc acgatatgca
    36841 ttggtataaa cagagcctga aattttataa tgtgaatacc agatgatatt tacgataccc
    36901 aaaagacata atgagatgaa aattaacaaa gtaggattat tagttattgt tttttttggt
    36961 gtcacaagaa aatacaatgc agatataatt ccagctgcat gaagaaactt tgttgcaaca
    37021 tcatcccata aagacaaagt cattaaagaa aaaatacata atatgagaat taaagacttt
    37081 ataccagaat taatttcatt ccaacaaaac gacatatagt ttcaacctat gctacgagga
    37141 tgggttattt aatgcaagat ctaacgttta ttcttttttc tatttaccag tcttaccatc
    37201 ctccagataa tataattttt tgatgctaat atttttgctc gtctcttatc tctcttgcca
    37261 ggaagattac aatcttttaa ttctttttca ttgctttcaa ctaattgatt caattgttta
    37321 aaatcaccat taagtagaac attccacaaa taacgagttt tatgataagc tctattttga
    37381 ctcaataatg tatgaatttc ttctttccgg ttacctttgt ataactgcat cacagaagag
    37441 tagatttttt cgtgcgcaaa tgactcatgt cgataatgag tgatggttct acatgagttc
    37501 attaaggaat tttctctata gttatgctta tacatataat atggaaccgc atacatatcg
    37561 catgtaatta gcagtcgata gataaaatcc agatcttctc ctagtcgtaa atcttcatca
    37621 aaaaaaagat tattctcttc cagaagttgt tttcttataa acaagcaccc tatatggaat
    37681 ctaacctttt tataaaaaaa gtcgaaaagt aaagatcctt cattaaactc tgtatttata
    37741 ggtgagtaag taacaccatt ttttatatga tggccataaa gcccacaata aatggcatct
    37801 cctcccgtct catgttgtct ggaatacatc ttcagcagat agttaggatc tatctcatca
    37861 tcatcatcca taaaacatat aaactgtcct tgcgccttgc ggatacctgt attcctggca
    37921 gacgaaacac cgccattttc tttattgata ataattaatc gagagtgggt taacccatac
    37981 tctctcaaaa cgtttgatgt gttgtcttta gaaccatcat taacaataac aatttcaaaa
    38041 tttgggtaag tttgtttttc taatttcgtt agagtttcag taatgtaatc agatgaatta
    38101 tacgtcggta ttattatgga tactaaaggg taatcattgc tcatcgtgtt ctcttgcctg
    38161 aactaataaa atatgatagt actcatcctt aattattatt gtaactcaga catccatgat
    38221 ttttattatt acccaaatag ttttctttta tacttattta atttaaattt aatgaagtaa
    38281 ataaaagcgt taaacgtatc accatttttc tttgcatgtt gatatttcac ttgatactgc
    38341 ttttcattcg tggctggaat aaacgccaca tcattccagc aagaagcctg ataagcctca
    38401 ttaaaatagt tctggcaggg ataattcgtc cagtcgttcc agggcttcgt tacgccaaca
    38461 taatgaatca gcttagtatc atcggtaatg gtctttttat aatcttcatc gcttttattt
    38521 tttaactcat aatcgatgcc ataaaagcaa tcataatcgc cgctaataaa aatacaatga
    38581 ccaaaaaaca gaatatttaa aatatcctga tcaaggcatt tatacttcgt gtgctgcggg
    38641 tcacttgcca gcatagcaat ggctttattc ataaaatcat ttttcgccca tgcttcaagc
    38701 tgcagatata ctacaccggc gttgaaataa ttccctggaa gtccctctat agccagacgc
    38761 ttcgctggtt tttcctgcat aaaaggcaca tctttaataa cagctgcaaa cttatcccca
    38821 tcaaaattaa tatcaagcaa tccagctaat gagcctttac agatgacatc agcatcgata
    38881 tataacagat tttcaatagt ttcactcaga tactggaacg caactaaacg gaagaatgtt
    38941 gcataagacc actgtttcag gctggggaag atatcaataa aggcattgga gactttataa
    39001 attcttactt caacgttagc atttttgctg gtaaaagcat taacacgttg tagataatct
    39061 tcatcaatgt agtcggtaaa gagatgaaac ttaaccgcaa ggtccgggtt atgcataaca
    39121 actgattgca tagatactgc cgcaccaaat aaaaagttac ggtcaatacc gtaagcgata
    39181 tttaacacac gatcggtgtt agcaatttcc ccagagacag cgacttcagt aatactctca
    39241 gctaataaat ccatgttggt tccgttttga ctgtgtggtt tacgctttgc cttttaattt
    39301 tttaataaat ttgcgtagtt tggttctgta gattactgaa tagtagccat aatcttttat
    39361 ttcgttggca atacccaggt gacgttccat ggccagacga tcacgagctt tacgctctgc
    39421 cgtacagctt ttaccggata ggtcaattat cctgacacca tctttactaa cgataaaatt
    39481 accccggtgt gggtcgcccg atagcatatt taaggcatgc aatttttcca ttgaagcctt
    39541 aatttctgct ttaacatttt ctggaataat cggcatatca ttgagctcta cgccctcgac
    39601 atactcgata agcatgataa aaacactggc atagttgaaa attttacgct cagccagaaa
    39661 ataaaaatca ttaggaaaag taagacctgc acttctgaca cgatcggttt caacgatcaa
    39721 attctgatag tagtcacctt ttacaaaaga ttttaaaaaa cgttcattcc ttttcgtttt
    39781 cggagcaaag actttaaaaa catagcgacc atatttcgta tcaatcaacg atactttggt
    39841 atcatcaata ttacgtaata cttttactgt tttaaaatta taggccagta tttcatcaaa
    39901 gatttcttta tatttattat cgttatcttt gtaataaaat gagaagccgc gatagcgtat
    39961 acttgtaatc ataagagtaa tttatttctt aagcttgtac ttaattaatg aagttatacc
    40021 tttaatatac tcaccatggg caaacagatg cttatattgt ttctgcattt ctgcaacagt
    40081 ccgcgcctct ttaagaggat atttcttcca gggagattgt tcacgcgcga tattaaagta
    40141 tgatgcagac ggatatccag cccagctatg ccatggttta gttatccctg tatagtgaat
    40201 caatactgtt ttatcagtaa tggtttgctg atactttcga tgactacgat catgaagttc
    40261 gtttttcagg gtataaatag tatcaaaatc cttcgcgagg tagatattat tcatattaaa
    40321 tgcgatattc aacgcatcct gatctaaata tttaagagag ccatatttag tttcccctcg
    40381 taaaagcgtg agtaaatacg gtgtcaaatt tgcttcatgc cattttttaa gattgacata
    40441 gataacgcct gcattgaaat aacgtccatt catttcggga atgttgaggc gttttgcaca
    40501 tgccgcctga gtactatcat tgtcaggaat gaccgcagca aactcatctt taaatataat
    40561 ttctgtgaga ggtttcaggc ttcctttaca aacaacatcg gcatccagat acagcagtgt
    40621 ggaaatactt tcactcagat attcaaaaga caataccctg aagtatgtcg cgtacgacca
    40681 aaactgtgac gtcggcagat cagcaaagta tttggggtca attaaatata caataattga
    40741 ggtattaaat tgttttgctg tttcattaaa tgactttaaa taattatcat ccacataatc
    40801 agtgaaaaca tgaaagacaa aattaatatc tgaattatta atcaaaacgg aagaaattga
    40861 aacgccagca ccatacaaaa aattcttatc gataccgtag gaaacattta gctctggggc
    40921 gttttctttt ttattatctg ctaaatacga aaaccgttct tttataaatt cattcattta
    40981 acttttctca atttcatttt attatcttta aataaaaata ataaatataa ttcattatcc
    41041 cgttaattgg tttattttgt ttaaaattat gcttggcgca ataacgagca tagtttgagt
    41101 taactggccg cattaacggt tcatttttcc agggcgatgc ttctttggct ttgataaatg
    41161 gttgcgcact tggataactg gcccagtaat gccagggttt tgtcgggccg acataatgaa
    41221 ttaatacgat ttcatcatta attggacaaa caaatgattt ttttaattca taatttaaac
    41281 taaactgcgt attgtattta gcatcaatga atttaacttt ccctaacagg ataagattaa
    41341 ggatatcttg atccatatag gttaaatggg aaacgatggc tttatcagca agcatcgaca
    41401 tcgctttagc agaaacggac tcctgcgccc acgctagtgt attaattaac aggacacctg
    41461 aattaaagta acccttttca agttcattac actgcagact ttgaccccgt aacgaccacc
    41521 agttagcatc gcgttcagta acaacagcag caacgttatt ggcaagatcc attgttatca
    41581 gcggctttaa gtttccctga caggcgatat cagcatccag gtaaaggatc ttatcttgtt
    41641 gatcaataaa gtaatctgca attacaaaac ggaaatacat ggcaatagac caatttttgg
    41701 tcgtcggtaa tgccttaagc cgttcacaat ttaccagatg gatctggata caggtacgat
    41761 agcttttcgc caattgggct aaacgctgga tatcggcttc agggatatca tcaataaaaa
    41821 cgtggaaaac aaaactcacg tcgttgttat gtaacaaaac tgacgtgatt gaaacaccac
    41881 aaccaaaaag aaagttttta tcaatgccat aagcaacatg gaatgatgat gccactgacg
    41941 ccgctggcct ttcatcgaag atgattgtct tattgatcat ctcttgtgga ttaaaatagt
    42001 gggcactcat atttctctcc ggaaattaca acgattttcg aatcgttctt tccctgattt
    42061 ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga atgtctttaa
    42121 gcggggcggc aaaatacact ttcataaacc gccagatatc ccgctgagtc aggccgatat
    42181 tcatcgaaga aaaataaagc ccaataagat ctttatcgcg ccaacgacgt ggaacgcgcg
    42241 tgcgaagctg cgcccggtgc aggtcaatta ccgaaatttt taactcctct tccttaccgg
    42301 aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acggtgatta atgcccgcag
    42361 catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt acgcgaacat
    42421 ctggtgggtt ggtcgcccag tcagcacagt aatcttccag acttatggtt ggtgtcagat
    42481 cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg ccgaatgcca
    42541 ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc cattcgcggt
    42601 ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct ttcagggtcg
    42661 tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc agagtacggc
    42721 gagtttccag ttcacgaaat atctcaccct gcaaggtttt aacttcctca aaaggatctt
    42781 taccgcgcca taacgtggca aacggctctt taagttcaac catccagacc acccgttatg
    42841 atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata atgtcgcgca
    42901 ttttccgccc aggcctggcg caatgaagat tgcgttaacg ctttgcgtaa aatctcattc
    42961 aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattggcctc gacaatataa
    43021 ggcgcatagc cacaaacggc tgttgttagt accggtaatc ctgcagtaat cgcttccagc
    43081 agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc cgccgccatt
    43141 aattccgaga catcattgcg ccccgagaag aagtgaacat tactgcgcac gccgcgtttt
    43201 tctgccagcg cctcaaattt tcgcggttta tcctgcccaa caacatatag caatgtgttg
    43261 tggcgcagcg aatccggtaa agaagcaagt gcttcaatgg aacgatcgac acctttacgc
    43321 gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc attcttctta
    43381 cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg ataaatcccc
    43441 ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa atcggcgatt
    43501 tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt tgcccgctca
    43561 aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt ctcctgcgct
    43621 actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg cattttgttg
    43681 aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca ggtaaaatac
    43741 tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcag ttcaaataca
    43801 tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc tcgggcggcg
    43861 actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa atatttatat
    43921 aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt aatttatcga
    43981 cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt cggtcacgct
    44041 gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg ttatttgacc
    44101 agggacgcca gaaaatatgg tctgtcgcac caaacagcga tatcagcgac gtattaactg
    44161 cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca tgatcgatta
    44221 acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt ggcgtctggc
    44281 atccctgcgc aatttcattg acgcaggcca gatcgtcttt atccgggccg gacgtcagaa
    44341 cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg gcgttgtccc
    44401 agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt ccaacaccag
    44461 cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc atggttgtct
    44521 gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt tccaccacat
    44581 ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca gactgccgat
    44641 gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt atagcaacca
    44701 tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat accttgatga
    44761 gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt atgccgtaga
    44821 gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa agcagcacat
    44881 cgatttttgc gtcagggtaa ttttttttca gcgaactaat gacgggagta gttaataaca
    44941 tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta tccacaagta
    45001 actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct accgccccag
    45061 gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt gtatcacttc
    45121 tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca ataaagaaat
    45181 caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga ttccttcctg
    45241 cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag atgttcagca
    45301 agaattggcg ggtaaattgc tatttaaatc aaaaacggga aaagtaatgg taaagccaca
    45361 gctaaataca tagaatcccc agcacatcca taagtcagct atttactatg ctcgaattgc
    45421 tttacaccgc ccttctctac cttattcagc cgctgatctg gatacggctc tgggtgcgcg
    45481 gacgtaaggc tccggcctat cgaaaacgct ggggtgaacg ttacggtttt taccgccatc
    45541 cgctaaaacc aggcggcatt atgctgcact ccgtctccgt cggtgaaact ctggcggcaa
    45601 tcccgttggt gcgcgcgctg cgtcatcgtt atcctgattt accgattacc gtaacaacca
    45661 tgacgccaac cggttcggag cgcgtacaat cggctttcgg gaaggatgtt cagcacgttt
    45721 atctgccgta tgatctgccc gatgcactca accgtttcct gaataaagtc gaccctaaac
    45781 tggtgttgat tatggaaacc gaactatggc ctaacctgat tgcggcgcta cataaacgta
    45841 aaattccgct ggtgatcgct aacgcgcgac tctctgcccg ctcggccgca ggttatgcca
    45901 aactgggtaa attcgtccgt cgcttgctgc gtcgtcttac gctgattgct gcgcaaaatg
    45961 aagaagatgg tgcacgtttt gtggcgctgg gcgcaaaaaa taatcaggtg accgttaccg
    46021 gtagcctgaa attcgatatt tctgtaacgc cgcagttggc tgctaaagcc gtgacgctgc
    46081 gccgccagtg ggcaccacac cgcccggtat ggattgccac cagcactcac gaaggcgaag
    46141 agagtgtggt gatcgccgca catcaggcat tgttacagca attcccgaat ttattgctca
    46201 tcctggtacc ccgtcatccg gaacgcttcc cggatgcgat taaccttgtc cgccaggctg
    46261 gactaagcta tatcacacgc tcttcagggg aagtcccctc caccagcacg caggttgtgg
    46321 ttggcgatac gatgggcgag ttgatgttac tgtatggcat tgccgatctc gcctttgttg
    46381 gcggttcact ggttgaacgt ggtgggcata atccgctgga agctgccgca cacgctattc
    46441 cggtattgat ggggccgcat acttttaact ttaaagacat ttgcgcgcgg ctggagcagg
    46501 caagcgggct gattaccgtt accgatgcca ctacgcttgc aaaagaggtt tcctctttac
    46561 tcaccgacgc cgattaccgt agtttctatg gccgtcatgc cgttgaagta ctgtatcaaa
    46621 accagggcgc gctacagcgt ctgcttcaac tgctggaacc ttacctgcca ccgaaaacgc
    46681 attgaggttg ttatgcaaaa acgggcgatt tatccgggta ctttcgatcc cattaccaat
    46741 ggtcatatcg atatcgtgac gcgcgccacg cagatgttcg atcacgttat tctggcgatt
    46801 gccgccagcc ccagtaaaaa accgatgttt accctggaag agcgtgtggc actggcacag
    46861 caggcaaccg cgcatctggg gaacgtggaa gtggtcgggt ttagtgattt gatggcgaac
    46921 ttcgcccgta atcaacacgc tacggtgctg attcgtggcc tgcgtgcggt ggcagatttt
    46981 gaatatgaaa tgcagctggc gcatatgaat cgccacttaa tgccggaact ggaaagtgtg
    47041 tttctgatgc cgtcgaaaga gtggtcgttt atctcttcat cgttggtgaa agaggtggcg
    47101 cgccatcagg gcgatgtcac ccatttcctg ccggagaatg tccatcaggc gctgatggcg
    47161 aagttagcgt agcgtttatg ccggatggta ttccatccgg cgcgcatgaa ttacttctgg
    47221 cactgccgac aataaaacgt tgcccgctgc gcatgtttag tcgccacaat cggcgtaccg
    47281 cacacccgac acggctcacc ttttcgcccg taaacctgca attcctgagc gaaatagccc
    47341 ggtttaccat cactttgcag aaaatctttc agcgttgtac caccctgctc aatcgaacgc
    47401 agcaacaccg ctttaatcac ccgagctaac aattcacact ctgccagcga cagtgatgac
    47461 gccagccgat ccggatggat ccccgccgca aacagtgatt cgctggcata gatattccct
    47521 acccctacca ccagcttgtt atccatcagc cacggtttaa tcgccgtttt tttcttcgcg
    47581 cacttctgat gcagatactc accattgaaa tcgtcgctaa gcggctccgg tccaagatgg
    47641 gtcagcacat tatgcccttc cagctctttg gtccacagcc aggcaccaaa gcggcgcgga
    47701 tcggtgtagc gcagcacttt gccgttgctc atcaccaaat ccacatggtc atgcttttca
    47761 gggggaagtt cttctggaag gatgcgcagg ctgccagaca tccccaaatg aatgataatc
    47821 cagccctcag gcagctccag cagcaaatat ttagcccgcc gctgcacgct aagcactggt
    47881 tggtcgctta aacggtagat ctcttctgaa accggccagc gcaagcgtcc gttgcgcacc
    47941 actgcatgaa gaatggttgc accaacgaga tgcggttcta tgccgcgacg gctggtttca
    48001 acttcgggta attcaggcat agcatctcca ggaatgaaca gatgcaatca atatgggggc
    48061 aagcagataa caaaaaaccc cgccggagcg aggttttttg ttacatcaaa gcgagaatta
    48121 tttgattttc gcttctttgt agatcacgtg ctgacgaaca actggatcga attttttcag
    48181 ttccagtttt tccggcttag tacgtttgtt cttcgtagtg gtatagaagt gaccagtacc
    48241 agcagaagaa accagcttga ttttctcacg aataccttta gccatgattt atttcctcta
    48301 agtacttagt acttttcgcc acgggcacgc agttcagcca gaactgtatc gatgcctttt
    48361 ttatcgatta cacgcatacc tttagcagat acgcgcaggg tgacaaaacg cttctcgctc
    48421 tcaacccaga aacggtgaga gtgcaggttc ggcaggaaac ggcgtttagt cgcgttcagt
    48481 gcgtgggaac ggttgttacc ggtcaccgga cgcttgccag taacttggca gactcgggac
    48541 atgtctattc tccaaaaatc aaattagctc gagcttcgta tggggtattg gcgcctcgtc
    48601 aggctttaca gcccggtcat cgcagttcta tgtgaactct cgattgccag gcccaaatgc
    48661 caaacccgag attctcaaag gtggcgtagt atacgctgac tcagcgatgt gctcaagtcc
    48721 cgaacagaca aagatcccga aggatcgcgc atagcgggtt aaatccagcc gcgttcggca
    48781 aaagaaacat actctccacg cccaatcacg atatggtcga gcacgcgtaa atccatgaac
    48841 tgacaactct ttattatccg ttcagtaatg agtttatccg ctttactggg ttcagcacaa
    48901 cccgaagggt gattatgtgc aaggatcagc gccgaggcgt ttatttttat cgcttcgcgg
    48961 ataatttccc gaggatggac ttcaacatgg tttagcgtgc cggaaaaaag acggctatgc
    49021 gttataaccc ggtgttgtga gtcgagaaag atcaccataa agatctcccg ctcctcaccc
    49081 gtgagctggc tttgtaaaaa ttcccgcgtc atctccgggc tgagtaaagg gctttcttca
    49141 cgcatccgca cgttgtagta acgccgcgcc agttcagcaa tcccctttaa ctgggcaaat
    49201 ttcgctacgc caattccatg aacgccacta aattgttcat attcagaggt taacaagcca
    49261 taaagagagc cgaaattctc cagcatctct tttgccaggg ttaatacatc tttaccgcgc
    49321 gttccggtac gcagaaataa cgccagcagc tcgacatccg ttaaggcgct aataccaaac
    49381 ttcagcattt tttcgcgcgg catcaacagc tgtgcattgt ttttcacctt cacctccttt
    49441 gtggtgctcg catcctgtca tagccgttcc gttaaatcga cggccagttt tcaatcctgg
    49501 aaagcgcctc gcaaagtgaa tctcaggtga aggcacgaca acaaaagtat tgtgataaaa
    49561 tcgccaactt ctggtgtcac cctacaggaa aaatcaacat gagcctggcc ggtaaaaaaa
    49621 tcgttctcgg cgttagcggc ggtattgctg cctataaaac ccctgaactg gtgcgtcgtt
    49681 tgcgcgatcg cggggccgac gtccgcgtag ccatgaccga agcggcaaaa gcctttatca
    49741 ccccacttag cttgcaggcg gtttctggtt atcccgtttc cgacagtttg ctggacccgg
    49801 cagccgaagc cgctatgggc catattgagc tgggtaaatg ggctgattta gtgattctcg
    49861 cccctgccac ggcagatttg attgcccgtg ttgctgccgg aatggcgaat gacctggtat
    49921 cgacgatttg tctggctaca cctgcgcctg tagccgtgct ccccgccatg aaccagcaga
    49981 tgtaccgtgc cgctgccacg cagcataatt tagaggtgct tgcttcccgt ggtttgctca
    50041 tctgggggcc agacagtggc agtcaggctt gtggtgatat cggtcctggg cgaatgctcg
    50101 atccgttaac cattgtggat atggcggtgg cgcatttttc gcccgtcaac gacctgaaac
    50161 atctgaacat tatgattacc gccggtccga cgcgtgaacc gctcgatccg gtgcgttata
    50221 tctctaatca cagctccggc aagatgggtt ttgctatcgc cgccgccgct gcccgtcgtg
    50281 gcgcgaacgt cacgctggta tcaggtccag tttcactacc gacgccaccg tttgttaaac
    50341 gtgttgatgt gatgaccgcg ctggaaatgg aagccgccgt gaatgcttct gtacagcagc
    50401 aaaatatttt tatcggttgc gccgccgtgg cggattatcg cgcagctacc gtggccccag
    50461 aaaaaatcaa aaagcaggcc acgcagggtg atgaattaac aataaaaatg gttaaaaacc
    50521 ccgatatcgt cgcaggcgtt gccgcactaa aagaccatcg accctacgtc gttggatttg
    50581 ccgccgaaac aaataatgtg gaagaatacg cccggcaaaa acgtatccgt aaaaaccttg
    50641 atctgatctg cgcgaacgat gtttcccagc caactcaagg atttaacagc gacaacaacg
    50701 cattacacct tttctggcag gacggagata aagtcttacc gcttgagcgc aaagagctcc
    50761 ttggccaatt attactcgac gagatcgtga cccgttatga tgaaaaaaat cgacgttaag
    50821 attctggacc cgcgcgttgg gaaggaattt ccgctcccga cttatgccac ctctggctct
    50881 gccggacttg acctgcgtgc ctgtctcgac gacgccgtag aactggctcc gggtgacact
    50941 acgctggttc cgaccgggct ggcgattcat attgccgatc cttcactggc ggcaatgatg
    51001 ctgccgcgct ccggattggg acataagcac ggtatcgtgc ttggtaacct ggtaggattg
    51061 atcgattctg actatcaggg ccagttgatg atttccgttt ggaaccgtgg tcaggacagc
    51121 ttcaccattc aacctggcga acgcatcgcc cagatgattt ttgttccggt agtacaggct
    51181 gaatttaatc tggtggaaga tttcgacgcc accgaccgcg gtgaaggcgg ctttggtcac
    51241 tctggtcgtc agtaacacat atgcatccga ataacgtcat aacatagccg caaacatttc
    51301 gtttgcggtc atagcgtggg tgccgcctgg caagtgctta ttttcagggg tattttgtaa
    51361 catggcagaa aaacaaactg cgaaaaggaa ccgtcgcgag gaaatacttc agtctctggc
    51421 gctgatgctg gaatccagcg atggaagcca acgtatcacg acggcaaaac tggccgcctc
    51481 tgtcggcgtt tccgaagcgg cactgtatcg ccacttcccc agtaagaccc gcatgttcga
    51541 tagcctgatt gagtttatcg aagatagcct gattactcgc atcaacctga ttctgaaaga
    51601 tgagaaagac accacagcgc gcctgcgtct gattgtgttg ctgcttctcg gttttggtga
    51661 gcgtaatcct ggcctgaccc gcatcctcac tggtcatgcg ctgatgtttg aacaggatcg
    51721 cctgcaaggg cgtatcaacc agctgttcga gcgtattgaa gcgcagctgc gtcaggtatt
    51781 gcgtgaaaag agaatgcgtg agggtgaagg ttacaccacc gatgaaaccc tgctggcaag
    51841 ccagatcctg gccttctgtg aaggtatgct gtcacgtttt gtccgcagcg aatttaaata
    51901 ccgcccgacg gatgattttg acgcccgctg gccgctaatt gccgcgcagt tgcagtaata
    51961 tgacgccgga tgacttttca tccggcgagt ttctttaaac gccaaactct tcgcgatagg
    52021 ccttaaccgc cgccagatgt tccgccattt ccggcttctc ttccagatag gcaatcagat
    52081 ctttcagggt gatgatagag atcactttgc agttgtaatc acgctcaact tcctgaatcg
    52141 ccgaaatctc gccgcgcccc cgttcctgac gatcaagcga aatcaatacg ccagcaagcg
    52201 tcgcgccatt agcctgaata atctccatcg attcgcgaat cgccgttccg gcggtgatca
    52261 catcatctac cagcattacg cgtccttgta acgcgctacc aaccagattg ccgccttcac
    52321 cgtggtcttt tgcctctttg cggttaaagc agtacggcag gtcaaggtca tgatgctccg
    52381 ccagcgccac ggcggtagtg gtcgcaatcg ggatcccttt gtaagcaggg ccaaacagca
    52441 gatcgaactc aatgccagaa tccaccaatg cttcagcgta aaaacggcct aacagtgcca
    52501 ggtcgcgccc ggtattaaac agcccggcgt tgaagaaata ggggcttttg cgcccggatt
    52561 tcagcgtaaa ctcgccaaac tttaacacct gcttgctaag cgcaaattca ataaactggc
    52621 gctgatatgg tttcatgcct tcgctcctca tcttactttt ctacagacaa aaaaaaggcg
    52681 actcatcagt cgccttaaaa atcagtttgc cagcgccgcc ttctgcgtcg ctacaatgga
    52741 ttcgattccc cctcgggcca gcgccaacaa ggtgagtagc tcttcatggg tgaacggctc
    52801 gccttctgcc gtcccctgca cttcaatgat gcgcccgtct tcggtcatca ctacgttcat
    52861 gtcggtctct gcggctgagt cttcaacgta ttccagatcg caaattgctt caccgtttac
    52921 aataccgaca gaaactgcgg ctaccatccc tttcatcggg ttggttttca gcttgccgtt
    52981 ttccaccagc ttctgtagcg catctgccag cgccacgcag gcacccgtaa tcgacgcggt
    53041 acgcgtgcca ccatcagcct gaagcacgtc gcagtccagc gtaatggtga actcacccag
    53101 cgctttcaaa tctactgccg cgcgaagagc acgggcgatc agacgctgga tttccattgt
    53161 gcgtccaccc tgcttacctt tcgccgcttc acgagcgtta cgggtgtggg tagaacgtgg
    53221 cagcatgccg tactctgcgg tgatccagcc ctggccctga cctttcagga agcgcggcac
    53281 gccttcttca atagaggcgg tacacaacac tttggtatcg ccaaattcga ccagcaccga
    53341 gccttctgca tgttttgtat agttacgagt cagggtaacg ggacgcacct gattattgct
    53401 acggcctgct ggacgcatat tgaaatctcc ggcttgaaac aaatgtggct gcgcattata
    53461 cggacttccg gcggttattc ctatcctgac aaggcatcga tggctataat ccttccacct
    53521 ctccttttat aaacaggaac gtctatgatc cgcagtatga ccgcctacgc ccggcgtgaa
    53581 atcaagggtg aatgggggag cgcaacctgg gaaatgcgct cggtaaacca gcgttatctg
    53641 gaaacttact ttcgtctgcc ggagcagttc cgtagccttg aacctgtcgt tcgcgagcgt
    53701 attcgttctc gcctgacgcg cggtaaagtg gaatgtaccc tgcgctatga gccagatgtt
    53761 agcgcacaag gtgagctgat cctcaatgaa aaactggcta aacagctggt aactgccgcg
    53821 aactgggtaa aaatgcagag tgacgaaggg gaaatcaacc cggttgatat tctgcgctgg
    53881 ccgggcgtga tggcagccca ggagcaggat ctcgacgcca ttgccgctga aattctcgcg
    53941 gcgctggatg gtacgctgga cgactttatt gtcgcgcgcg aaaccgaagg tcaggcactg
    54001 aaagcattga tcgagcagcg tctggaaggc gtcaccgccg aagtggtcaa agtccgcgcc
    54061 catatgccgg aaatcctgca atggcagcgt gagcgtctgg tcgcgaagct ggaagatgct
    54121 caggttcagt tggaaaacaa ccgtctggag caggagctgg ttctgctggc acaacgaatt
    54181 gacgttgccg aagaactgga ccgcctggaa gcgcatgtca aagagaccta caacattctg
    54241 aagaaaaaag aagccgttgg tcgtcgtctg gactttatga tgcaggaatt caaccgcgag
    54301 tcgaacactc ttgcgtcgaa gtctatcaat gccgaagtga caaactccgc gattgagctg
    54361 aaagtgttga ttgagcagat gcgcgagcaa attcagaata ttgagtaact cgtaacacta
    54421 attcttactt ttcagccttc acaaatgccg cccactcaaa cagagcggca tttttcttcc
    54481 ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt ttttgcccag
    54541 cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga tttactttct
    54601 ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt ttgaagaaat
    54661 acggcattat ggcactgaag ggcaagaatt ttggtcagct agagaattgg caccgttact
    54721 ggattatcgg gactggcgca attttcaaaa ggtactggcc cgcgcgactc aagcgtgcga
    54781 agccagcaat caagctgcat ctgaccattt cgttgaaacc accaaaatgg ttgtattagg
    54841 ttcaggggct caacgagaac ttgaagatgt tcatctctcc cgctatgcct gttacctggt
    54901 agtacaaaac ggcgaccctg cgaaaccggt tattgcggca gggcaaactt attttgctat
    54961 ccagacccga cggcaggagc tggctgacga tgaagctttc aggtaacttc gtgaagacga
    55021 aaagcgtctt tttctgagaa atgaattgaa agaacataac aaacaattgg ttgaggcagc
    55081 acagcaagct ggggtagcga cagctactga tttcgccata tttcagaatc atggttacca
    55141 agggctgtat ggtggattag atcagaaagc catccaccaa ctgaaggggc tgaaaaagag
    55201 tcagaagatc ctggatcata tgggttcaac agaactggcg gctaatctct ttcgagctac
    55261 ccaaacagaa gaaaaactca agcgggatgg cgtgaattca aaacagcaag ctaataccac
    55321 acactttgac gtgggtagca aggtgaggca aaccattcag gaacttggcg gaaccatgcc
    55381 tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg taaaaattac
    55441 agagaagaaa tagcagaaac taaaaactct ttttgttgat tgagacaccc aatgcgtaag
    55501 ccaaggtcca ggtgcagtga acacaatggc taaatattgc acctttcttt tcccccctca
    55561 gttttaacct attttttctt atgcattttc tcagacaaga agtcagaaga atgcatctct
    55621 gctacagaaa atagcgattt cacataacta caagttatca atttcccctc accttaaaaa
    55681 atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt tagggagaga
    55741 accatgctgt tacacatttt gtatttggtt ggcattactg ccgaagccat gacgggggcg
    55801 ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc gaccgccacc
    55861 gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct cggctgggtc
    55921 aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac tatcgttgct
    55981 cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg actggtggtc
    56041 ttttctatca tcggggcaca ggttgcactg gatatggggc acggcccaat tattgccgtt
    56101 gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt ctgtaaacgc
    56161 atcccactgg tattccagaa agagttgtat gccggtgtct catttgcctc cgccgtgctg
    56221 tacatcgcgc tgcaacacta tgtttctaat catgatgtgg taattatctc caccctggta
    56281 ttcggctttt ttgcccgctt actggcgcta cgccttaagc tgggattacc ggtcttttac
    56341 tacagccacg aaggacacta aggttcaaaa cctgtgatct gctgggcagc cagccaactg
    56401 cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc gcgccccgat
    56461 ccagtgcccg gcagctgctg ccagaactgc tccgtgctaa ataaaagttg cgaccaggac
    56521 cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc catcacccag
    56581 cgagtaaaag gctgcttacg agccagatta aactgatgcc atagctgcgc acttttactt
    56641 ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca ggaaaagata
    56701 tgctcaaagc gatgagtctg atgcagcgcg cgccagccgg cctcaccaat gccatccagc
    56761 ccaagaacct gttttgcccc cagccagact aagcgtgaaa tgaactgttc ctgacaaaca
    56821 tcagaagcaa agtagcaggt caacgagtta aagcggtttt ctggcggtgt cggttttgta
    56881 cgttctgcac cgcgccacac cacatcatca atgcgaggaa taccctgacc ggcaaggctg
    56941 acgagaatct gatcaccagg cgcaatatcc cactcctgcc agcgcctgac ggaaccaata
    57001 ttcacccgct ggactttttt atcatccagc atgacaggtg cgagtgacgc aaccaccgat
    57061 attttaccgc tcttacccac cgcaaactga attgccttca cttcggcaac ctgagctaca
    57121 ggttgatatt tccaggccac cagccactct gcctggcccg gtagccaatg gcgggattct
    57181 ggctctttcg ccgctcgtac aactacgcca tcggtgacga agggtaattc cgctttccac
    57241 cactcattgc gtacgcgcgc aacttcatca gcatttttca ccgcacgggt atacgtctgc
    57301 gttagagtaa aacctgcggt agccagctct tttaaacgat cagacattaa ctgcggtcca
    57361 tccggccatg cccagagaat tcagcgtgtc gctatcgtcc tggcgcatca tcaagccagc
    57421 aacttttgcg cgggcattta ttccccccat ttgttgttgg atatgcccct cgcgctggag
    57481 aaatatttcc ccctgaagcg tactgttggc taaaggcccg ctaacggttt gcggcacagc
    57541 ggaaattaag ctaactttct gcgtccagtc ctcgcctttc aggccattac cgcgactgat
    57601 tgctttgttc agtttcccgt cccgataaac cagggttacc gcaacgccat caacttttgg
    57661 ctgcacccaa agatcgctac gttctcgcat ccacagactt aatgcgtttt tatccaccat
    57721 tttgcgtaca cccgtatggg caaccggatg cataaccgca ccatttaaag gcggcatcat
    57781 cacgtcgcgg ggctcgctcc caaagcagcg ttgccactgc gtaagacggg cgcttaactg
    57841 atcgtaaaca ccgtcttcca cctcactttt tccttccttc cagtagtcat cgtcccactg
    57901 ttttatttgc tgttgcaggc gggaaatttc ttcctgtgct ctggctggcg accaggccgg
    57961 acagaccgcc cacacagatg attgccagca caagatactt attaatatcg ccatccatac
    58021 tttcatcatc acctccgctg taggtagtca ggcagatata caacgtgatg aaagcaaagc
    58081 cgagtggcaa aaacggagtc tgcgaggacg cttcctgaaa atcgtcttta ttgcagtgaa
    58141 tgacaggcaa atgcggaagc agctacgcaa aacgcaacaa ctttgcgcaa aaagtgtgag
    58201 caagggctac gtcacatggc cgcgccgtgt ataataagct cgtatgtagg ctttatttcg
    58261 ctaatcacat acgaaagata ctcatggctc aaggcacgct ttatattgtt tctgccccca
    58321 gtggcgcggg taaatccagc ctgattcagg ctttattaaa aacccaaccg ttgtatgaca
    58381 cccaggtttc tgtttcacac accacacgcc aaccgcgtcc tggtgaagtc cacggtgaac
    58441 attatttctt tgttaatcat gatgaattta aagaaatgat tagcagagat gcgttcctcg
    58501 aacacgcaga agtttttggt aattactatg gcacttcgcg tgaggccatt gagcaagtac
    58561 tggcgaccgg tgtcgatgtt tttctcgata tcgactggca gggcgcgcag caaattcgcc
    58621 agaagatgcc gcacgcgcgg agtatcttta ttttaccgcc gtctaaaatt gaactggacc
    58681 gccgtctacg cggtcgcggt caggacagcg aagaggtcat tgcaaagcgt atggcgcaag
    58741 ctgttgcaga aatgagccat tacgccgaat atgattacct gattgtgaat gatgacttcg
    58801 ataccgcgtt gaccgatttg aagaccatta ttcgcgccga acgtctgcgc atgagccgcc
    58861 aaaagcagcg tcatgacgct ttaatcagca aattgttggc agactgaacc tgatttcagt
    58921 atcatgccca gtcatttctt cacctgtgga gctttttaag tatggcacgc gtaactgttc
    58981 aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt actggtcgcc gcgcgtcgcg
    59041 ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc ggaagaaaac gataaaacca
    59101 ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa caaccagatc ctcgacgttc
    59161 gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt acaagccgtt accgctattg
    59221 ctgaaggtcg tcgttaatca caaagcgggt cgcccttgta tctgtttgaa agcctgaatc
    59281 aactgattca aacctacctg ccggaagacc aaatcaagcg tctgcggcag gcgtatctcg
    59341 ttgcacgtga tgctcacgag gggcaaacac gttcaagcgg tgaaccctat atcacgcacc
    59401 cggtagcggt tgcctgcatt ctggccgaga tgaaactcga ctatgaaacg ctgatggcgg
    59461 cgctgctgca tgacgtgatt gaagatactc ccgccaccta ccaggatatg gaacagcttt
    59521 ttggtaaaag cgtcgccgag ctggtagagg gggtgtcgaa acttgataaa ctcaagttcc
    59581 gcgataagaa agaggcgcag gccgaaaact ttcgcaagat gattatggcg atggtgcagg
    59641 atatccgcgt catcctcatc aaacttgccg accgtaccca caacatgcgc acgctgggct
    59701 cacttcgccc ggacaaacgt cgccgcatcg cccgtgaaac tctcgaaatt tatagcccgc
    59761 tggcgcaccg tttaggtatc caccacatta aaaccgaact cgaagagctg ggttttgagg
    59821 cgctgtatcc caaccgttat cgcgtaatca aagaagtggt gaaagccgcg cgcggcaacc
    59881 gtaaagagat gatccagaag attctttctg aaatcgaagg gcgtttgcag gaagcgggaa
    59941 taccgtgccg cgtcagtggt cgcgagaagc atctttattc gatttactgc aaaatggtgc
    60001 tcaaagagca gcgttttcac tcgatcatgg acatctacgc tttccgcgtg atcgtcaatg
    60061 attctgacac ctgttatcgc gtgctgggcc agatgcacag cctgtacaag ccgcgtccgg
    60121 gccgcgtgaa agactatatc gccattccaa aagcgaacgg ctatcagtct ttgcacacct
    60181 cgatgatcgg cccgcacggt gtgccggttg aggtccagat ccgtaccgaa gatatggacc
    60241 agatggcgga gatgggtgtt gccgcgcact gggcttataa agagcacggc gaaaccagta
    60301 ctaccgcaca aatccgcgcc cagcgctgga tgcaaagcct gctggagctg caacagagcg
    60361 ccggtagttc gtttgaattt atcgagagcg ttaaatccga tctcttcccg gatgagattt
    60421 acgttttcac accggaaggg cgcattgtcg agctgcctgc cggtgcaacg cccgtcgact
    60481 tcgcttatgc agtgcatacc gatatcggcc atgcctgcgt gggcgcacgc gttgaccgcc
    60541 agccttaccc gctgtcgcag ccgcttacca gcggtcaaac cgttgaaatc attaccgctc
    60601 cgggcgctcg cccgaatgcc gcgtggctga actttgtcgt tagctcgaaa gcgcgcgcca
    60661 aaattcgtca gttgctgaaa aacctcaagc gtgatgattc tgtaagcctg ggccgtcgtc
    60721 tgctcaacca tgctttgggt ggtagccgta agctgaatga aatcccgcag gaaaatattc
    60781 agcgcgagct ggatcgcatg aagctggcaa cgcttgacga tctgctggca gaaatcggac
    60841 ttggtaacgc aatgagcgtg gtggtcgcga aaaatctgca acatggggac gcctccattc
    60901 caccggcaac ccaaagccac ggacatctgc ccattaaagg tgccgatggc gtgctgatca
    60961 cctttgcgaa atgctgccgc cctattcctg gcgacccgat tatcgcccac gtcagccccg
    61021 gtaaaggtct ggtgatccac catgaatcct gccgtaatat ccgtggctac cagaaagagc
    61081 cagagaagtt tatggctgtg gaatgggata aagagacggc gcaggagttc atcaccgaaa
    61141 tcaaggtgga gatgttcaat catcagggtg cgctggcaaa cctgacggcg gcaattaaca
    61201 ccacgacttc gaatattcaa agtttgaata cggaagagaa agatggtcgc gtctacagcg
    61261 cctttattcg tctgaccgct cgtgaccgtg tgcatctggc gaatatcatg cgcaaaatcc
    61321 gcgtgatgcc agacgtgatt aaagtcaccc gaaaccgaaa ttaatgtttt atgaacccaa
    61381 cacgttatgt acgcatctgc gaaatgctcg ccaggcggca gcctgatctg accgtctgca
    61441 tggagcaggt ccacaaacct cataacgttt ctgcgattat tcgtaccgca gatgccgttg
    61501 gcgtacatga agttcacgcc gtctggcctg gtagccgcat gcgcaccatg gcttcggcag
    61561 cggcgggtag taacagctgg gtacaggtga aaacacaccg caccattggc gatgccgtcg
    61621 ctcatctcaa aggccagggc atgcagattc tggcaaccca tctttctgat aacgctgtcg
    61681 atttccgcga aattgattac actcgcccga cctgcatttt gatgggacag gagaaaacgg
    61741 gcatcacgca ggaagcattg gccctggcgg atcaggacat catcattccg atgatcggca
    61801 tggtgcagtc gctgaatgtt tccgttgcct cagccctcat tctttacgaa gcccagcgtc
    61861 agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat gttgccggaa gcagagcaac
    61921 aacgcctgtt gtttgaaggc ggctatccgg tgctggcgaa agtcgcaaaa cgcaaaggcc
    61981 tgccttatcc ccacgtcaat cagcaaggcg agatcgaagc tgatgccgac tggtgggcta
    62041 ctatgcaggc tgcagggtaa gtgccatgaa aggtcgcctg ttagatgctg tcccactcag
    62101 ttccctaacg ggcgttggcg cagcacttag taacaaactg gcgaaaatca acctgcatac
    62161 cgtgcaggat ctactcttac accttcccct gcgctacgaa gatcgcaccc atctctaccc
    62221 catcggagaa ctactgccgg gcgtttatgc cacggtggaa ggcgaagtgc tgaactgcaa
    62281 tatctccttc ggcggtcggc ggatgatgac ctgccagatc agcgacggtt ccggcatcct
    62341 caccatgcgc tttttcaatt tcagcgcggc aatgaaaaat agcctggcgg cgggccgccg
    62401 tgtactggct tatggagaag caaagcgcgg taaatatggt gcggagatga tccacccgga
    62461 ataccgcgtg caaggcgatc tcagcacgcc agagttacag gaaacgctca cgccggttta
    62521 cccaactacg gaaggcgtaa agcaggccac gctgcgtaaa ttaaccgacc aggcgctgga
    62581 tctgctcgac acctgcgcca ttgaagaact cctgccgccg gaactgtcac aaggaatgat
    62641 gacgctgcca gaagcgttgc gcactttgca tcgcccgcca ccgactttgc agcttagcga
    62701 tctggaaacc ggacagcatc cggcgcaacg tcgtctgatt ctggaagaac tgctggcgca
    62761 taacctcagc atgttagcct tacgcgccgg agcgcaacgt ttccatgccc agccgctgag
    62821 cgccaatgac acgctgaaaa ataaacttct tgccgcctta ccgttcaagc caacgggcgc
    62881 tcaggctcgc gtggtggcgg agatcgagcg cgatttggcg ctggatgtgc cgatgatgcg
    62941 cctggtacag ggcgatgtag gttccggtaa aacgctggtc gctgccctcg ccgcgctgcg
    63001 tgcgattgcc cacggcaaac aggtggcatt gatggcacca accgaattac tcgccgaaca
    63061 gcacgccaat aacttccgta actggtttgc accgcttggt atcgaagtgg gctggctcgc
    63121 cggtaagcag aaaggtaaag cgcggctggc acagcaggaa gctatcgcca gcggtcaggt
    63181 gcagatgatt gtcggtacac acgccatctt ccaggaacag gtgctgttta acggcctggc
    63241 gctggtgatt atcgacgaac agcatcgttt tggcgtgcat cagcgtctgg cgttatggga
    63301 gaaaggccag cagcaaggct tccatcctca tcagttgatc atgaccgcca cgccgatccc
    63361 ccgaacgctg gcaatgactg cgtatgctga tctcgatacc tcggtgatag atgagctgcc
    63421 gccaggccgc acgccagtga ctacggtcgc tattcctgat acccgccgta ccgacattat
    63481 tgaccgcgtg cgccacgcct gcataactga aggtcgtcag gcatactggg tttgtacgtt
    63541 gattgaagag tcggaattgc tggaagcgca ggcggcggaa gctacctggg aagagttgaa
    63601 actggcgcta ccagagttga acgttggcct ggtacacggg cggatgaaac ctgccgagaa
    63661 acaggcggtg atggcgtcgt ttaaacaagg tgagctacac ctgctggttg ccacaaccgt
    63721 tattgaagtc ggcgttgatg tgcctaacgc cagtctgatg attatcgaaa acccggagcg
    63781 tctgggtctg gcgcagttac accagctgcg cgggcgcgta ggtcgtggcg cggtggcttc
    63841 tcactgcgtg ctgctctaca aaacgccgct ttctaaaacg gcgcaaattc gcctgcaagt
    63901 gctgcgcgac agtaacgacg gttttgtgat tgcgcaaaaa gatctggaga ttcgcggccc
    63961 tggcgaattg ttaggcacgc gtcagacggg taatgctgaa tttaaagtgg cggatttact
    64021 gcgcgatcag gcgatgatcc cggaagttca gcgcctggca cgccatattc acgaacgtta
    64081 cccacaacag gcaaaagccc tgatagaacg ctggatgccg gagacggaac gttactcgaa
    64141 tgcgtaaaag gcggcagtga gaagaccgcc atttcaggtt accctacctt cctgccggat
    64201 gcgattcatc accctacaaa ttcaataaat tatgaatcaa tacgcaggct tgataagcga
    64261 agcgtatcag gcatttgtct ttgttgcggc ggcacttcta ccgccgcttc atcggttaac
    64321 cggcaaaaat cggcaacatc aaatacaact taattaccag cgcattgacg atatcgataa
    64381 agaacgcacc gaccatcggc accaccaaaa acgccatgtg cgacgggcca aaacgttcag
    64441 tgatcgcctg catgttggcg attgccgttg gcgttgcacc gaggccaaaa ccacagtgac
    64501 ccgcagccag cactgccgca tcgtagtttt tgcccatcat gcgccaggta acgaatatgg
    64561 catacaacgc catgaagatg gtctgtacca ccagaatcgc cagcatcggc agcgccagcg
    64621 aagccagctc ccacagtttc agccccatca acgccatcgc caggaacaac gacaagctta
    64681 cgttacccag cacggatacc gcacgctcaa agacgcggta aaagcccatc attgacagac
    64741 cgttgctcag aatcacgcca acaaacagta cacagacgaa ggtcggcagt tcaaaagcag
    64801 tgccagccaa aagttgcgca acaattttcc ccaccgtcag gcagatagca atcagcgcga
    64861 tagtttcaat cagcaccaac gaggtgatca tgcggcccac atccggcttt tcaaacgccg
    64921 tcgggacttc ctggtcatcc ggaataccgt tcggcgtggt ggagtgtttc accagatagc
    64981 gcgctaccgg accgccaatc aagccgccca gcaccaaacc gaacgttgca caggccatcg
    65041 ccacttctgt cgcattggtg aagccataac gttcaatgaa caatttactc cacgcagcgc
    65101 ccgtaccgtg accgccggaa agcgtaatag aaccggccaa cagccccatc agcggatcaa
    65161 gccctaacag gctagccata ccaatgccaa tggcattttg catcaccaac agaccaacaa
    65221 ccacaatcaa gaagatgcca accacacgcc caccggcacg caaactggca atgttggcgt
    65281 tcaggccaat ggtggcgaag aaagccagca ttaacggatc gcgcagggac atatcaaagt
    65341 tgacttccca gcccatgctt tttttcagta ccagtagcgc tagcgccacc aacaaaccac
    65401 ccgcaacagg ttccggtatg gtgtatttct tcaaaaagga gacggaatgg accaacttac
    65461 gcccgagcag caacgtcagc gttgcggcaa caagcgttgc taaagtatcg agatgaaaca
    65521 tagttactcc tttgtatccg catcactctt tcatactcgt catacttcat cgcgcagtgg
    65581 caacctcatg aactatgacg ggtatcgagt tatgctttcc tgggtcaaat tctcagctgt
    65641 taatgaacaa atggcatgga ttttaagcag aaaaatacca aaagttatat aaaaaagtgc
    65701 atttatctca tttaaatatt tagatgagga tgtggcaaac gtttgctttt ctctttcggt
    65761 cagctaaaat gcccgctttg ctaccacggg attgttttcg atgtctgttt ccaccctcga
    65821 gtcagaaaat gcgcaaccgg ttgcgcagac tcaaaacagc gaactgattt accgtcttga
    65881 agatcgtccg ccgcttcctc aaaccctgtt tgccgcctgt cagcatctgc tggcgatgtt
    65941 cgttgcggtg atcacgccag cgctattaat ctgccaggcg ctgggtttac cggcacaaga
    66001 cacgcaacac attattagta tgtcgctgtt tgcctccggt gtggcatcga ttattcaaat
    66061 taaggcctgg ggtccggttg gctccgggct gttgtctatt cagggcacca gcttcaactt
    66121 tgttgccccg ctgattatgg gcggtaccgc gctgaaaacc ggtggtgctg atgttcctac
    66181 catgatggcg gctttgttcg gcacgttgat gctggcaagt tgcaccgaga tggtgatctc
    66241 ccgcgttctg catctggcgc gccgcattat tacgccgctg gtttctggcg ttgtggtgat
    66301 gattatcggc ctgtcgctaa ttcaggttgg gttaacgtcc attggcggcg gttacgcagc
    66361 catgagcgat aacaccttcg gcgcaccgaa aaatctgctg ctggcaggcg tggtcttagc
    66421 cttaattatc ctgcttaacc gtcaacgtaa cccttactta cgcgtggcct cactggtgat
    66481 tgcgatggcg gccggatatg cgctggcgtg gtttatgggc atgttgccag aaagcaacga
    66541 accgatgacg caagaactga ttatggtgcc aacgccgctc tattacggtc ttggcattga
    66601 atggagtctg ctgctgccgc tgatgctggt ctttatgatc acttcgctgg aaaccattgg
    66661 cgatatcacg gcgacctctg acgtttccga acagccagtg tccggtccgc tgtacatgaa
    66721 acgcctgaaa ggtggcgtgc tggcaaacgg cctgaactcg tttgtttcgg cggtgtttaa
    66781 caccttcccg aactcctgct tcgggcaaaa caacggagtg atccagttga ctggtgttgc
    66841 cagccgctat gtcggttttg tcgtcgcgct gatgttgatc gtgctgggtc tgttcccggc
    66901 agtgagcggt tttgtacaac acattccaga accggttctg ggcggcgcaa cgcttgtaat
    66961 gtttggcacc atcgccgcct ccggtgtgcg tatcgtttct cgtgagccgc tgaaccgtcg
    67021 ggcgattctg attatcgcgc tgtcgctggc ggttggtctg ggcgtgtctc agcagccgct
    67081 gattttgcag tttgcccctg aatggctgaa aaacctgctc tcctccggga tcgccgcggg
    67141 cggtattact gccatcgtgc tgaatctgat tttcccacca gaaaaacagt aattcactgg
    67201 cggcggtaag ataattaccg ccgcagtctc acctctttaa ccattcccgc cttgaggatt
    67261 gcgcgtaaat cgtgcataac tcccttatgt gcatttcacg ggatggaaga ccatgaaatt
    67321 tattgggaag ctgcttctct acattctcat cgctctgtta gtggcgatcg ctggcctcta
    67381 ttttcttctg caaacccgct ggggagcaga acatatcagc gcatgggttt ccgagaatag
    67441 cgactatcat ctggccttcg gggcgatgga tcaccgtttt tccgcgccat ctcatatcgt
    67501 gctggagaac gtcacgtttg gtcgtgatgg ccagcccgcg accctggtgg ccaaaagtgt
    67561 cgacattgcg ctaagcagtc ggcaactgac cgaaccacgc catgtcgata ccatcctgct
    67621 ggaaaacggg acgctgaatc tcaccgacca gaccgcgccg ctaccgttca aagccgatcg
    67681 tctgcaactg cgtgatatgg cgtttaatag cccgaatagc gaatggaaac tgagcgcgca
    67741 gcgggtaaat ggcggcgtag ttccgtggtc accaaaagcc ggtaaagtgc tgggtacgaa
    67801 ggcgcagatt cagtttagtg ccggatcgct ttcgctcaat gatgttcctg ccaccaatgt
    67861 actgattgaa ggcagtattg ataacgatcg cgttacgctg actaacctgg gtgccgacat
    67921 cgcgcgcggg acattaaccg gaaacgcgca gcgtaacgcc gacggcagct ggcaagtgga
    67981 aaacctgcgc atggcggata tccgtctaca aagcgaaaaa tcgctaaccg acttctttgc
    68041 gccattacgc tctgtcccgt cgttgcagat tggtcgcctg gaagtgatcg atgctcgttt
    68101 gcaaggtccg gactgggcgg tgaccgacct cgatctcagc ttgcgcaaca tgaccttcag
    68161 taaagatgac tggcagacac aagaaggcaa actgtcgatg aacgctagcg agttcattta
    68221 tggttcgctg catttatttg acccgattat aaacgcggaa ttttccccgc agggcgtagc
    68281 gctgcgccag ttcaccagcc gctgggaagg gggtatggtc agaacgtcag ggaactggct
    68341 gcgtgacggg aaaacgttga tccttgatga tgcggcaatt gccgggctgg aatatacctt
    68401 gccgaaaaac tggcaacagt tgtggatgga aacgacaccc ggttggttaa acagcctgca
    68461 actgaagaga tttagcgcca gccgcaatct gatcattgat atcgaccctg acttcccgtg
    68521 gcagctcacc acgctcgatg gttacggtgc caacctgacg ctggttaccg atcataaatg
    68581 gggcgtctgg agtggctcgg cgaatctgaa tgccgccgcc gcgacattca atcgtgttga
    68641 tgttcgtcgc ccgtcgctgg cgctgaccgc caacagcagc acggtgaata tcagcgaact
    68701 gagtgcattt actgaaaaag gcattctgga agccactgcc agtgtttcac aaacgccaca
    68761 acgtcagacc catatcagcc tgaatggacg cggtgtgccg gtgaatattt tgcaacaatg
    68821 gggatggcct gaattaccgt tgactggcga cggcaatatt cagcttaccg ccagtggaga
    68881 tattcaggcc aatgccccgc tgaaacctac ggttagcggg caattgcatg ccgtgaacgc
    68941 cgcaaagcag caagtgactc aaaccatgaa tgctggcatc gtttccagcg gtgaagttac
    69001 atcgacggag ccggtgcggt aaacatcaat ctcgccgggt acgactcaga cgtacccggc
    69061 attccatcaa tagattacaa cgtaattgtc agcgcattcc cttgaggctt caccaccagc
    69121 ccctgctcac tttcagcctg cgaaccgtct tgcagaccat ttactttcac aacattgcgc
    69181 aggcacagtg tccagttctt cgcctcgccc gcaccagtca cagtaatcgt gttgccagta
    69241 cgtgctgctt ttaaagtaaa gatcaccgat ccgtcagcag cgggcacttc acagacggct
    69301 tcatgcccgt cttgcagatt aaagaggtgg aatgccgtgc cttcgtgcca cacgtaatcg
    69361 ggacgttgat cgttgttgcc cagcgccagt agagtgttat cacgcacata aacgggcaga
    69421 ctcaggaagc cgtgctgctg tttatgccag cgactaccgt cgagttcatc gttgtgccac
    69481 aggtgtgtcc agcgaccttc cggcaggtag aactgcacat cgcccgcttc agtgaacacc
    69541 ggcgcaacca tcacgttgtc gcctaacatg tattgacggt caaggtaatc acaagccgga
    69601 tcgtccggga actccatcat catggcccgc atcatcggcg taccccgcgc gttcgcacgc
    69661 gcagcttcac gatacagata cggcatcatg cggcatttca gttgcgtgaa gaagcgcacc
    69721 acatcacagg actcatcatc gtaggcccac ggcacacgat aagatttgct accgtgtaaa
    69781 cggctatggc tggagagcaa accaaacgcg caccagcgtt tgtaaacgtg cgccggagcg
    69841 gtattttcaa agccgccgat atcgtggctc cagaagccaa aacctgaaag gccaatagac
    69901 aaaccaccgc gcaggctttc cgccattgat tcgtagttag cgtaacaatc gccaccccag
    69961 tgtaccggga atttctgcgc accgacggag gccgagcggg caaacaagac agcttcttcc
    70021 tcaccaacgg tgtccttgag cacgttccac accagttcgt tgtagatgta cgcataatgg
    70081 ttatgcattt tctgcggatc ggaaccgtca aaccactgaa catcagttgg gatacgttcg
    70141 ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca gacctttcag tttgtcggcg
    70201 taccatttgc aggcatccgg attggtaaag tcataaatcg ccagacctgg ctgccattta
    70261 tcccactgcc atagcgaacc gtccgggcgt ttgagtaaat agcctttctc ttgtaactct
    70321 ttaaagacgg gggatttttg accgatatag gggttaatcc agacgcagat tttcagccct
    70381 ttcgctttca ggcggcggat catcccttcc gggtcaggga aagtcagcgg gtcccactca
    70441 aaatcgcacc actggaaggc tttcatccag aaacagtcaa agtggaaaac atgcagcggc
    70501 agattgcgtt ccgccatacc atcgataaag ctgtttaccg tcgcttcgtc gtagttggtg
    70561 gtaaatgaag tggttagcca caggccgaag aacacgcggg cggcagcgcc ggacgaccag
    70621 taaagcgggt ataacgatca agtaccgctt tcggcgtcgg gccgtcgata acaaagtatt
    70681 cgagatattc actctcaacg ctgaactgca ctttggagac tttctccgat cccacttcaa
    70741 aagagacaca ctggggatga ttgaccagta ccccataacc acggttagtc atgtagaacg
    70801 gaatattttt atacgcctgt tcagtacttg tgccgccgtc ccggttccag gtctctaccg
    70861 tctggccatt gcgcaccagg gcagtaaagc gctctcccag accgtaaact gtttcgccaa
    70921 cgccaagatc aagccgctca aacatataat tgcgttgatt attcgtgtcc tgcacgtagc
    70981 cattattttt cacctgacta ccggtaatac gttcgccgtt gcgcagaaaa tccagtgacc
    71041 agaactcacc tttgctgaca cgcgcgctta agttgccact tttaaactca gcataacgtt
    71101 ctgtgttttc gattgtgacc ttcacgtcct gcaaaatatt gagcggataa tgaggaccgt
    71161 tattcagcgc cccctgaaaa tgctcaatcc gcacaccgac aataccttcc tgtggggaga
    71221 aaaagcgcag cgtaaataaa ggcgtgtcaa gctgccaggt acgttcacgt acatcacggg
    71281 gggcagcata gaccaccatt tcattatcct gctgttcaac ctcgaacacc tgaagcgggt
    71341 gaatcaaatt gaggccaggt tgaatcaacc agtttccatc gctaattttc attgccgttc
    71401 cttagttctg cacttcttga gaggtgaatt gttgctggca acggcgttta ccctgagcca
    71461 gctgttccat aacggttttc agattgtgcg tcgtgagtga gtagtagcgt ttagcgataa
    71521 tcgcgctcag caaataacag atcgccggaa caatcgtgaa tagcgcaata atgatgctaa
    71581 tcgtggcgct gttctgcgct ttttctgccg catcatatcc gccataagcc agcatccagc
    71641 cgataagagc gccgccgaag gccaacccca gtttgagcac aaacagcgtg ccagcaaaac
    71701 tgatcccggt caggcgttta ccattgcacc actcgccgta gtcgacggta tcggacatca
    71761 ttacccactg gataggtgtc accagttgat gcaacacacc aatcacgaag atgaagacaa
    71821 acatagtgat gctggcctgc atgggaacaa agaacatcgc gaggctaatc actgccagca
    71881 gggcgttcgt ccaccagaag atagtgactt tacatttcca gtcggtcaga ggttttgcca
    71941 gtgcggaacc aatcaggtta cccacgcaat aagtggtgag aaaagcgaca aacacttccg
    72001 gcgtgcccaa aatccatgtg acgtaataca tcatcgcccc accgcgtacg cacaccgcca
    72061 ggatattgaa aatggttagt aaaccgacaa tccgccactg gtcgttttgc cagatatcac
    72121 gtaaatcttc ccgcatagac gttgttgtag gtggtgcttc aacgcgttct ttagtggtga
    72181 agaaacaaaa tgccagcatc atgaatgcca ccacggaaag gaccgcgata ccgccctgga
    72241 aaccgagtgg tttattatca cccccaatta aattaaccag tggcatcatc agaacagtag
    72301 aaagcatgcc tcccgcggtc gccagcacaa aacgccagga ttgcagcgag atacgctgag
    72361 tcgggtcatt ggtgattaca ccacccaatg cgcagtaagg gatattgacg acggtataaa
    72421 gtaaggtaag tagggtgtaa gtaattgctg catagatcat tttgccgttc atactgagat
    72481 ctggcgtgct ataggccagt acacagacga tcccgaatgg cagtgcgcca aacagtaccc
    72541 acggacgaaa tttaccccag cgagagcgcg ttcgatcggc caacaacccc atgcaaggat
    72601 cggaaatcgc atccagtgca cgagcgacca aaaacatggt tccgacaaaa ccggcaggaa
    72661 tgccaaaaat atcggtataa aagaacatca tatataacat tacgttatcg aaaataatgt
    72721 ggctggcggc gtctcccatg ccataaccaa ttttctcttt aacggacaac acttcactct
    72781 tcatctgctt ttccttgatg ctataggggt gctgagaccg ataacgcatt ttgtaaacca
    72841 tccctgtgag agcgaatatt ccgatatctg gttattaatt tatgattctt gttttatgtg
    72901 atcgtggtag cgttaattcc gctcatatat cattgtaaaa tatgggtttt atatgaacta
    72961 taatgctttc gtgataatac gctgcgtgta ttaggcggaa aaaactgatc tgggggatgt
    73021 agaaactcaa ggaagtagct ataatgcgcc ccgcctccat gtagcaatcg aggcgcggaa
    73081 gatcgtcgtc tccggtgagg cggctggact tcaaatccag ttggggccgc cagcggtccc
    73141 gggcaggttc gactcctgtg atcttccgcc aaaatgcctc tcctgacgtt ctccaacccc
    73201 ttgtaatact gtcaatccag atattttcta ctgctcaata ttcaatcggc ttcaggaata
    73261 cctggtctaa ggttttgtga tggacgatga gcggctgaag aatccgcctg tcggttcttc
    73321 tgtcctcgaa cgcatccgcg atattcacgc cagtatgcgc cgtgtttatt tgcaggtgat
    73381 ttgcccccct gctgactctc agccaactgt atcgccatta aggtgaagat gttgttttac
    73441 tcgccccaga acaccctttt ttatacgact attctatttc tttaaccgct ggcgaaaaaa
    73501 actgactata aacttcacca ataatcagac tgccatcaag attaatttgt tcatttaata
    73561 tatcaaatca acttgtttta tttttactat caaaaatagc tcattgcctt actataatta
    73621 atatataaat tcggtaatta attcttaaca tgcttttgct aataatctca attgctggcc
    73681 ctataatatt gcgctagcat tgctttctgg ttgtatcagc gatactcaaa aattcttaat
    73741 acaatactca ttcgactggt acttatttgt aactcagtta tattttttcg cccggtgatt
    73801 cagaagaatg caaaaaacgg ctaccactcc atcaaaaata cttgatctca ctgccgcggc
    73861 atttttactt gtcgcctttc tgacgggtat tgcgggcgct cttcagactc ctaccctaag
    73921 tatattcctc gcagatgaac tgaaagcccg tcctataatg gtaggttttt tcttcaccgg
    73981 tagcgctatt atgggaattc tggtcagtca atttctggca aggcactccg ataaacaagg
    74041 cgaccgtaaa ttactgattc tgctatgttg cttatttgga gtgctggcct gcacgctttt
    74101 tgcgtggaat cgcaactact tcattctcct ctcaacgggc gtacttctga gtagttttgc
    74161 ttccaccgca aacccgcaaa tgttcgccct cgcccgtgaa cacgccgaca gaacaggccg
    74221 tgagacggtc atgttcagta catttttacg tgctcagatc tcgcttgcct gggttatcgg
    74281 gccaccgctc gcttatgaac tggcaatggg atttagtttt aaagtgatgt atctcaccgc
    74341 tgccatcgca tttgttgttt gcgggctgat agtctggttg tttttgccat caatacaaag
    74401 aaatattcct gtcgttaccc aacccgtaga aattttaccc tccacccaca ggaagcggga
    74461 tacgcggcta ctttttgtgg tctgttcaat gatgtgggcg gcgaataatc tctacatgat
    74521 aaatatgccg ctatttatta ttgatgaact gcatctaacc gataaactgg ctggagaaat
    74581 gattggtatc gctgccggtc tggaaattcc gatgatgtta atcgcaggct attacatgaa
    74641 acgtattggc aagcgactat taatgctcat tgctatcgtg agtggaatgt gtttttacgc
    74701 cagcgtactc atggcgacga ctccggcggt tgagctggaa ttgcaaattc ttaatgccat
    74761 cttccttggt attctctgtg gtatcggcat gctttatttt caggacctga tgcctgaaaa
    74821 aataggctct gcgacaacgt tatatgcaaa tacttcacgc gtcggctgga ttatcgccgg
    74881 ctctgttgac ggaattatgg ttgaaatctg gagctaccat gcgttgttct ggctggcgat
    74941 agggatgttg ggtattgcga taatttgcct gctgtttatt aaagatattt agctcaccag
    75001 tctgggctaa gaaaaatcgg cgtggcgcaa acagagccat cctcgccatt tttgctatca
    75061 tgcctgcata cataaacgac aaaacagtat gcagagggaa aaatgggttc caccagaaag
    75121 gggatgctga acgttctgat tgccgccgtg ttgtggggaa gttcaggggt ctgcgcgcaa
    75181 tacatcatgg agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg tttgatattc
    75241 gccggtttga ttctactgac gctgtcattt gttcatggcg ataaaatctt ttctattatt
    75301 aacaatcata aagatgccat tagcctgctg attttttccg tggttggcgc gctaactgta
    75361 cagctcactt ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac ggtgctgcaa
    75421 ttcctctcac cgacgattat cgtcgcctgg ttctcactgg tgcgtaaatc gcgcccgggc
    75481 attctggttt tctgcgctat tttgacatcg ctggtcggga cttttttatt ggtgacacac
    75541 ggtaatccga cgtcattatc gatctctcct gccgcgttgt tctggggcat tgcctcggca
    75601 tttgctgctg cattctatac cacctatccc tcaacgctaa ttgcccgcta tggcacgttg
    75661 tctgtcgtcg gctggagtat gctgatcggc ggtctgattc tgttgccttt ttatgccaga
    75721 caagggacaa actttgtcgt taacggcagt ttgattttgg cgttttttta tttgatcgtc
    75781 attggtacgt ccctgacatt tagtctgtac ctgaaaggag cacaattaat tggcggtcca
    75841 aaagccagca ttttgagctg tgcagaacca ttaagtagcg cgctactctc tttgctgttg
    75901 ctggggatca cgttcacatt accggactgg ctgggaacgc tgctgattct gtcatcggta
    75961 attttgattt caatggattc ccgtcgccgc gccagaaaaa taaatcgtcc ggcgcgggat
    76021 gagtgagaat taccagccag gcaccgcgcc accgttaaag atggtttctg ccgctttagc
    76081 gacttcggat gattgataag attgcagaaa ttccttcacg ttttctgcat tcttattatc
    76141 ttcccgtgcc accaaaatat tcacatacgg cgaattttta tcttcaataa atacgctgtc
    76201 gtgcaccgga gaaagcccgg tctgctgaat gtaagtggtg ctgataatcg ccacatcaac
    76261 tttgggatca tccagtacgc gcgggagctg cgccccctcg agttccataa tctgcaaatg
    76321 acgaggatta tcggttatat ccagcgcggt aggtaataac ccttttccct ctttcagagt
    76381 aattagcttc tctttttgca acagtaaaag tgcgcgacca aggttggtcg ggtcgttggg
    76441 gatcgccact gtcgcgcctt cttttatttg cgccacggtt ttgatttttt tggaataacc
    76501 cgccataggg aagacaaacg tatttcccac cgctaccagt ttataaccat gcgcctgatt
    76561 atcttgttca agaaagggtc gatgctggaa gacattcgca tcaagttcgc catgatttgt
    76621 cgcatcgttc ggtagcaacg aaccgctaaa tcctaccagt tcaacatcga ggccatattt
    76681 ctcttttgcc acctttttag cgacttccgc gacatcttgt tctgcgccat ttataacgcc
    76741 aactttaatg tgttttgcat cgctgctact ctggtcgcaa cctgccagca gaattccggc
    76801 cagcaataat gcggcccctg tccgtagatg atgtgttgtc agtttcattt ttttatcctt
    76861 ttattaatga ttacctgact atagcggtaa ggtcgctgcg gtttaaaaaa cgaaacgcta
    76921 tcgataagaa taaaaaggaa taaaagtgga atataaagtc tggcacttct tactgaccac
    76981 gcaagcgcgt ttcgtacagc atgatgagag cgatgaaagc aaactacact tatgcttcat
    77041 tcgatacaca tttgtaaaag gttgaatcca tgaaaccaac gacactactg cttattttca
    77101 cttttttcgc tatgccaggc atcgtatacg ccgagtcgcc ttttagctca ctgcagtccg
    77161 caaaagagaa aaccacggta ttacaagatt tacgtaaaat ttgcacgcca caggcgtcat
    77221 tatcagatga agcgtgggaa aagttaatgt tgtcagatga aaacaataaa caacatatac
    77281 gcgaggccat cgtggcgatg gaacgtaata atcagagcaa ttattgggaa gcgttgggta
    77341 aggtagaatg cccggatatg taagaacagc aacaactact gaccaacgct tctttacctg
    77401 atatcaggat ttcttcattt tcacctttgc agtaaccaac aatgcagtca gcaacatcaa
    77461 tgtgccggac aacatcaacg gcgaagtcag accaatatta tccagcgcat aaccgccgat
    77521 tgccgcgcca caggtattag caagctgaat aaccgccacc tgaatagacc cggctttttc
    77581 tgcctgatcg gccagcgagc gggtgatcca cgttgaccag ccgacgggaa ccaatgcaaa
    77641 agttagcccc cagataatcg ccacgccggt agcaacgatt ttatcgcttc cccacaacgt
    77701 cagtaccaac gcactcacag ccagtattaa cggcgcgcct gctaaggcca gttttaccga
    77761 acgtttaaga atgaacgacg aaagcgacgt accaataaag ctggcgatac caaaactcaa
    77821 cagcaccagc gttaagccat ccacgccgaa tcccgccagg ttcatataca ccgggcgaat
    77881 atacgtgaag aaagcaaact gcccggcgaa agacatgaag atggcgatca tccctgccat
    77941 cacacccgga cgttgtaata agcggaaagt attttgtttc tgatgcgagg gttcgcctgg
    78001 cagtgaaggc aatgatttga taatccagaa aatacacagc acgcccatca ccgccgccgc
    78061 attaaagaca ttgcgccaac cgataagctc gcctaaaaaa ctgcccaacg gcgcggcaat
    78121 caccagcgca atagaaaccg cgccgaagat caccgacagc gccttcggca ccgtacgcgg
    78181 cggcaccaga cgcatggtca gcgacgccga catcgcccag aacccgccca gcgccagccc
    78241 cagacaggca cgaccgatta aaagcaaact gaatgagtta gcaaaggaaa ccagcaagca
    78301 ggagagcgtc agcaaaacgg caaacaaaat aacaacgtag cggcggtcag tagcctgaat
    78361 tgtctgggta ataaacaaac tggcaaacat tgccacaaag gcggtcacgg tcaccgattg
    78421 cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt ggcgtcaaca aactgacggg
    78481 caaaaactca acgataatca gacaggcgac acaaaacgcc accgagaaaa cggctgacca
    78541 gttcggtcgg gtgatggcat ccgcgccgcg gttttcggca ataaattcac tcatggtgtt
    78601 acccgtggca tgtttcaggg gaaaagccga ccactttaac attgaaatgt gtgacgcatt
    78661 taacgttttt gcaactttta cggtgttgct tgagctggag ttaagatcga aacggaacag
    78721 ggcaaagatt accggatgcg gcgtgaacgt cttatccacc cgacaggtca ggcgcggtct
    78781 gtaggctgat aagacgaaac ggtttcaatg caaccagtga acgccctcgg caggctgaaa
    78841 gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc atttcaggtt cggtttcgcg
    78901 cagaaaacct agcgccagcc tgacttgagc gtcggtaatc ggcgcggcga ggcgagcttt
    78961 ttgtaaaatc cgatgccatt gcagcaggtt ctgttcgcta ccatcgacaa tgacaatttg
    79021 ccagatgagc aacacctgcg cggcgttttg caggtgcgac tcatccggac gatgcaaata
    79081 acgtaaaaat tcgtcaccag ctgactttcc catttgatcg accatcgact ccacgggtac
    79141 gacatcgatg ttcagccgat cgctgagacg gcgaatcgct tttctggccc cggatgtcag
    79201 cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg agcattatcc agatcatgag
    79261 ttctcctgcg taatcagaac tcatgatatc tggaaaccct cgccagttac taatccacca
    79321 gaataatctt cagtgcaaac agagctgcga ccaccaccac acacaggttc agatcgcgcc
    79381 agcgcccggt gcatactttc atgatgcagt acgacataaa gccgagtgca atcccttcgg
    79441 tgatcgagaa agtaaagggc atcatcaccg tggtaataaa cgcaggcacc gattcggtaa
    79501 aatcatccca gttaacgcgc gccaggctcg aagtcatcag cacgccaaca aagattaacg
    79561 ctccggcggt tgcgtaagga ggaactatcg ccaccagcgg tgagaagaac ataaccaaca
    79621 ggaacataac gccaaccaca accgcagtca gccccgtgcg gccaccgact gccacaccag
    79681 aagtactttc aatataggca gtaacagacg aggtgccgat aaacgcaccc gccaccgaac
    79741 tgacgctatc aacatacagc gccttattca tattggggaa tttaccgtta ccatctatta
    79801 agcccgcttt atcagttaca ccaattaatg ttcctgatga atcaaatagg ttgatcagca
    79861 taaaggagaa aatgataccg gcgagttcaa gtgttaacgc gccgctcaaa tctacttcac
    79921 caatgacgcc gctaatatca ggcggaatgg aatagacgcc gctaaaatga acatcaccga
    79981 aaaataatcc acagcaagac gtcaccacaa tagaaaccag caccgcggca tgaaaatgac
    80041 gtgatgacaa cacggttata ataaaaaacc ctaaaatacc taacaacacg ccgtgagaac
    80101 ttaaatcgcc aatcatcacc agcgtgtctt tattggcgac aataacgcca gtatttttta
    80161 atcccattaa ggcgataaat aatccaattc cgctggtgat accaatacgt aaacttaagg
    80221 gaatgttgga gatcatccag taccggatac gaaacagcgt gagcaaaaat agtccaactg
    80281 cgccccagaa tattgcgccc atcccggtct gccaggagat gcccatcgcc cccacgacca
    80341 cgaaggcaaa gaaggcgttc agccccattg ccggagccag cgccacgggt aagttagcaa
    80401 atatccccat cgcaatactg ccgataccgg caatcaaaca ggtggtaaca aacaccactt
    80461 tcgggtccat ttgtgccgcg ccgaggattt gcgggttcac aaaaacgatg tacaccatgg
    80521 ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac ggtggtccca tgctgaggta
    80581 gtttaaataa tcgcgaaagc gtccctgatt cattactcac gtaatcggta ttgtcattat
    80641 tcattttttt gtccattgga ggagatttaa tcccttgctt ttgtaaaaca aatgacaaca
    80701 acggttcagt gataattatc acatttcaat tgcacattaa tggatattct ttaataatct
    80761 cgcgacgttt ctttatgata aataataatc aaattgataa aatcaaaatg agaaaaatat
    80821 gaataattct attaaccata aatttcatca cattagccgg gctgaatacc aggaattgtt
    80881 agccgtttcc cgtggcgacg ctgttgccga ttatattatt gataatgtct ctattctcga
    80941 cctgatcaat ggcggagaaa tttccggccc aattgtgatt aaaggacgtt acattgccgg
    81001 tgttggcgca gaatacgctg atgctccggc tttgcagcgg attgatgctc gcggcgcaac
    81061 ggcggtgcca gggtttattg atgctcacct gcatattgaa tccagcatga tgacgccggt
    81121 cacttttgaa accgctaccc tgccgcgcgg cctgacgacc gttatttgcg accctcatga
    81181 aatcgtcaac gtgatgggag aagccggatt cgcctggttt gcccgctgtg ccgaacaggc
    81241 aaggcaaaac cagtacttac aggtcagctc ttgcgtaccc gccctggaag gctgcgatgt
    81301 taacggtgcc agttttaccc ttgaacagat gctcgcctgg cgggaccatc cgcaggttac
    81361 cggccttgca gaaatgatgg actaccctgg cgtaattagc gggcagaatg cgctgctcga
    81421 taaactggat gcatttcgcc acctgacgct ggacggtcac tgcccgggtt tgggtggtaa
    81481 agaacttaac gcctatatta ctgcgggtat tgaaaactgc cacgaaagtt atcagctgga
    81541 agaaggacgc cggaaattac aactcggcat gtcgttgatg atccgcgaag ggtccgctgc
    81601 ccgcaatctc aacgcgctgg caccgttgat caacgaattt aacagcccgc aatgcatgct
    81661 ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa ggacacatcg atgccttaat
    81721 tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg gcatatcgcg tcgccagctg
    81781 gtcgacggcg cgccactttg gtctgaatca cctcggctta ctggcacccg gcaagcaggc
    81841 cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg cagcaggtac tggtgaaagg
    81901 cgagccgatt gatgcgcaaa ccttacaggc ggaagagtcg gcgagactgg cacaatccgc
    81961 tccgccatat ggcaacacca ttgcccgcca gccagtttcc gtcagcgact ttgccctgca
    82021 atttacgccc ggaaaacgct atcgggtcat tgacgtcatc cataacgaat tgattacgca
    82081 ctcccactcc agcgtctaca gcgaaaatgg ttttgatcgc gatgatgtga gctttattgc
    82141 cgtacttgag cgttacgggc aacggctggc tccggcttgt ggtttgcttg gcggctttgg
    82201 actgaatgaa ggtgcgctgg ctgcgacggt cagccatgac agccataata ttgtggtgat
    82261 cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag gtgattcagg atggcggcgg
    82321 gctgtgcgtg gtacgtaacg gccaggtcca aagtcatctg ccgttgccca ttgccggcct
    82381 gatgagcacc gacacggcgc agtcgctggc ggaacagatt gacgccttga aagccgccgc
    82441 ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag atggcgtttc tttctctgcc
    82501 agtgatcccc gcgctaaaac taaccagtca ggggctattt gatggcgaga agtttgcctt
    82561 cactacgctg gaagtcacgg aataataaaa aagcccggcg tcatgccggg caaaagtcac
    82621 cagttacgtt tatgccactg tcaactgctg aatttttttc tcgcggcgga ttttgcgttc
    82681 ttccatcacc gccactatcg ccatcaggca gatacaacca atcgcggcga tatcaagtgc
    82741 ggcgaaggtg cctgcccagc cggtaaggcc gaataccggc gtcccatcgg caatcattcc
    82801 cagacctaac ttggcaaagc tgtcaccaat caggtaagca aaggtgcctt taataccatc
    82861 ggcagcgcca atcgcttttt taggtacaaa gccaacagca gccacaccaa tcaacaattg
    82921 cgggccaaag accaggaaac ccaacgcaaa gagagaagcc agatagatat attcgttgct
    82981 ggcatgttga tacacaccga gcgtggcgat aatcagcgcc agcgcgatgc aggccaccag
    83041 gccacggcga ccgttcgcca ggtcagagag ccagccccac agcagcgtac cgaccagcgc
    83101 accagcttca aacagcgtaa agccctgaat cgccaccgct ttagagagtt tcagttcctg
    83161 gaacgcgtat acggttgacc actggtcgat accaatacgt accacataga ggaaaatgtt
    83221 ggcgaagcac agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca
    83281 cttggtcata tcggtagatt ctgtctcttt gtcctcttcg ctgatctcct cgccgaacag
    83341 ttcttcagct ttgccgaggc cataagattc cggggagtcg ctgccgtaac gcaggccgat
    83401 aaaaccgaca atcagcgcga taatcgacgg gaagataaac attccgatga catggccatc
    83461 gaacaggtaa tttgccccga acagcgccac acctgctgcg cctgcaccgc caaggttgtg
    83521 agaaatattc cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc atttggtgat
    83581 ggtggagtaa ctgcacgaac cgccggtact ctggaaaaag ccgcttaagg cgtagaaggc
    83641 aatcatcagg aacaggctaa ccgagccgct gcccatactg gcactgaagc ccagcataca
    83701 aatagcagag aggatcagca tgaacggcag gaattgtttg gtgtttttgc cgtcggcgta
    83761 gtaggaaacc agcgttttac ccacgccata agtgatggag aaacccaggc cgatcatccc
    83821 cagctgcgtc atgctcaacc cgtaggtcga aatcatatcg ttctgcgcga tgttaaagtt
    83881 cttgcgaatc aggtacatcg tcaggtagcc gataaagacc accaggtagg attgcatgaa
    83941 cggtttgaac cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcgaac
    84001 ctggtttaag aaagccagca tgggttactc ctgaaatgaa tacctgcgtg aggcatgcat
    84061 tgtaaaaatc agcagcaaat caggcctgag atagcgtcca ggcaaaacct ggaaaatttc
    84121 ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc acgcttcgcg cggtgtctgg
    84181 gcgttcaaaa agggcagtaa cagcagtgcg gaaatcccgg cggcgataga gataaccaca
    84241 aaaaatccgc tccagtgcca ggtatcgagt actttcgcca gcggccaacc agcaagcgac
    84301 gcccccagat aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc ctctttgtgg
    84361 gaacactctg ccgccgccat accgattaac atctgtgggc caaagacaaa aaaaccaatg
    84421 gtgaagaagc aggttgcctg catcacgtag ctggcaaatg gcatcagcca cagggagccg
    84481 actgaaagca aaattccggc ggcgaaaatc aaattcatcg gccctcggtt gccgttaaac
    84541 aatttgtccg agccccaacc cgctaccagc gcaccgataa atccacccag ttcaaacatc
    84601 gtcactgccg tattcgccgt gaccagatcg acgcccagcg tctcggacat atacaaattg
    84661 ccccagtcgt tgatcgccgc ccggaccaca tagaccagca catagcaaaa cgaaagcagc
    84721 cagatatacg gattcagcaa cacatatttg gtgaggatct ctttacgcgt cagccctgcc
    84781 ccttcttgtt gttgagctat ctccagcgcg tcgtgtcgcc attcaccgac cgccggtaaa
    84841 cctaacgcct gcgggcgatc gcgcagccgc cagcagagaa aaattcccac gacgatcgcc
    84901 atacaaccag caatcatcat cccggcacgc cagccgtaat gtagtgcagc cgctgccatc
    84961 acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc ccaccaaccg
    85021 ccgcgctcgg tacgagaata ccaggcggtt aacagacgcg cacacaccgg tgaaccccag
    85081 ccctggaaaa aggcgttcag cacccagagt acggcaaacg cccatagcga cgtcgagaag
    85141 ccaaacagaa tgttgataat gcccgtggcg ataagcccta tccccataaa ataacgggca
    85201 tttgagcgat cgctgacaat gccggagaca aacttcgaca cgccataggt aatgtaaaac
    85261 agggtcgcta acaggccgat atcgctacgg ctgagcacgc cgttagcaag gatttctggt
    85321 acggcggcgt taaaactttt ccgcgtgaag taaaacagcg cgtaacccag ccagatggtc
    85381 agcagaatat gccgacgcca gtagcgatag cgggcatcaa tttcatattt atcagtcatt
    85441 aatggcgcat cggcaggcgc tttcagaaac ggcaacatca tcaaacctta gacatagcgt
    85501 tgaggtagag aaacgctgac acgcgtgccg tgcagacagg aaatgtgtaa tgtgccaccc
    85561 agcgccgtta cgcgctcgcg cattccggtg aggccaaaac cttgttgccc ggaacccggc
    85621 ggcaaaccgc tgccatcgtc ttcaataacc agcatcaacc gttcatcctg ctgccagcct
    85681 tgcagggtga ccgcgctggc atcagcatgt ttcacaatgt tgttcagccc ttcctggcag
    85741 acacgaaaca gcgtcacgcg ctggttttcg cttaacgctg attcatcgat tcgccattcg
    85801 agatggctga caataccgcg cccttccagc tccatttccc gcatcagtga gcggatggcc
    85861 tgctccaggg tgagatcatc caactggcgc ggacgtaacc gacccaacaa acggcgcacc
    85921 gcgtcgtaaa cgcccagcga tagttgttcg atgagctgcc cgctctgctt cacgctggcg
    85981 ttatctgccg ccagccgctg aacaatgccc gcctgagtac gaatagcagt gatggtctga
    86041 ccgatatcat catgcagctc acgcgccaca tcacggcgca cgctctcttc ggtttccagc
    86101 aaccgttcag ccagatgctg attgcgcgcc agttcctttt gcagcgactg gttaagttca
    86161 cgcaaccgct ggatgccagc gccaagcaac aaccctgtca gactttgcac cagcagcgag
    86221 agcaataaat ccaccggatg atcgcgccag gtttgactgg cgatcagcgc gatggcgttc
    86281 atcaacgtcg caatcagcgc cccttgccaa ccatagtgcc aggccagcgc gataatcggc
    86341 agcgccagac agaatggcgt aaagcgcgac agttcgtccg gcaatcccaa ctggagccag
    86401 agactgataa caaacagcag caagtaccag accagatgtc gcccgcgcca gttgattggc
    86461 tgagaaacca gtgacgggcc gagcggcagc caggtgttat tggcgagata gtgccagaac
    86521 accagacata tcggggccag cgtcaggccg ccagttaaag tcagcaacag cgcattccac
    86581 gactctttgc cgtgccaaag ccagggcagc gactgcaaca acgccgccgc cgttaacgcc
    86641 gccccctgta acagcaaggt gcgccagtca cgctgatggc gatagcgcga gatcagcgct
    86701 acgggcagta acgtcagtaa actaccgatc atcaataacg gaaaatgggt taaaccgacc
    86761 gcctgcgtta gccagtaaat cagcagccac tccgcgccca gcagcaccgg ccagtatccg
    86821 cgcgggcatt gcagcattag ccccagacgc agaccaaacg gaaataacag caccgccata
    86881 tcagggcgct caaccagatg caggctgata ctccacaggc aaaaccatgc ggcagagaag
    86941 ataaaaaagc aggcaataac ggtaattaag cgggagaaca acgtcttcat caccagccat
    87001 caaacatgcg gcgcgccagc tctacgtcgt tactgacgcc cagtttttcc atcagattgg
    87061 cgcgatggac gtgtaccgtt ttcggtgaca agcccagttc ggcggcaatc tctttcaccg
    87121 ccattccttg cgccagtttt tccgccacct ggcgttcacg tttggttagc gggtcctgac
    87181 gaccggatgc cagtttaatg gcaatatccg gcgtcagata acagccgccc gtggcaaccg
    87241 tatgcaccgc agcaatgagt tcatccgggc tacagcgttt ggaaagaaag ccgcgtgccc
    87301 ccgcgttaag cgcctgctca accagcgcag gactgtcgtg aacggagagc ataatcgtcg
    87361 ccataccttt cggcagctgg cttagcagct ccagaccgga gatatcgggc atggagatat
    87421 cgcaaataca cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg cgccccgaac
    87481 caaactcggc aactacctgc aaatcaggtt ccagccccag cagctgcgca aagccggagc
    87541 ggacgatgag gtgatcgtct ataagggcaa cggtgatcat ggtcttgtcc tggcgggtaa
    87601 aaaaaaacgc gcttacctta acgataagcg cgatgttgtt caagccttga gcggttactg
    87661 aaaaaacacc gcgatcttgt taaacatcgt cggatcggac tgattacgct gcactttcac
    87721 gacatcttcc agcttatcga tttggcttat catctgctcc agacgctggt cgtcattgac
    87781 cagtagccag atatggcttt tgtcgctgtc ctgaatcggc agacaaagaa tgccttcaac
    87841 gttaaaagcg cggcgggcaa aaaggccaca aacgtgggtc attacgcccg gatggttgcg
    87901 aacggtgagc tccagaatta cgttgtcatg agttgtgttt tgcatggctt attcccccac
    87961 catttcagta ttcgccgcac ctggcggcac catcgggtaa actttttctt cggcatcaat
    88021 gcgcacatgg atcagcgccg ggccagggcg attgatgatt tcctgcaatg cagcctgcgg
    88081 atcggcttcg ttattcaaat cacaggtttc gaggccgaat ccggcggcaa tctgcataaa
    88141 gttgattttg cccggatagg tggcggcaaa aacgccttgc tcgtagaaca gactctgttg
    88201 ctgatgcacc agccccagcg cttcgttgtt catcagaatg attttgacat ccagctgatt
    88261 ttcactggcg gtcgccatct cctgaatatt catcatcagg ctgccgtcgc cggagaaaca
    88321 caacactttg cgatccgggt tcgccagcgc cgcgccaatc gccgcaggca ggccaaaacc
    88381 catcgtgccc agcccaccgg aggtcagcca ctggcgtggg cgattgagcg gataagcttg
    88441 cgcggtccac atctgatgct gccccacatc ggtggtgata atcgcattgt catcgacaca
    88501 ggcggcaacg gcgttgatca ggccgtaatg gcttaatgga tcgcacgctt tcgggattgg
    88561 acacggaaac tcacgctgca aatccgctac caactggtgc cactctgcac gcggttgcgc
    88621 ttccaccagc gggatcaact gcgccagcac gtcatcaaca tccgcctgaa tcgccacatg
    88681 cggctgcttg attttaccca gctctgcacg gtcgatatcg acatgaatga ttttggcatt
    88741 cggacagaac tgctcggttt tgccaatcgc ccggtcatca aaacgcgcac cgagcacaat
    88801 cagtaaatcc gcctcctgca agatatagtt agtgctgcgc acgccgtgca tccccagcat
    88861 acccagcgac aacggatgcg cttttggcag catgcccagc gccattaaag tcatggtggt
    88921 aggcagttgc gctttctccg ccagttcacg cacccgcgca ggcgcattga tcacaccacc
    88981 acccagataa agcaccgggc gtttggcagc gttaatcatt gtagctgcgt cacgaatgct
    89041 ttcttcgcta aaggcgggtg cagcggcttt ttctgccacc gcgggctgag cttcaatctc
    89101 aaaaaccgcc gtttgcacat ccttaggaat gtctatccac accgggcctg ggcggcctga
    89161 ttgcgcaatg cggaaggcat cgctcatgac ctgcgggagt tcttcgatat gtctgaccag
    89221 atagttgtgt ttggtgatgg ggatagagat gccgtaggtg tcgacttcct ggaaggcgtc
    89281 ggtgccgatc atcgaggcgg gaacctgacc agtgatgcaa atcagcggga tggagtccag
    89341 ccgcgcatcg gcaatggcgg tcaccaggtt agtcgcaccc ggtccgctac aggccataca
    89401 gaccgccggt ttaccgtcgg tgcgcgccat tccctgagcg ataaatcccg cgccctgttc
    89461 atggcgagcc agaatatggc ggatttgcgt actttggctt aaggcatcgt aaacaggcag
    89521 gatagaaccg cccggaatgc ccgtcacaat cttaatgccc tgctgttcca ggaaatgaac
    89581 gataaattct gcgccggtaa agcgcttacg cgtcgatgtt gtgcccgaac ttgccatgct
    89641 ccagtccttt tcttctgggc cgactttccg ggaggtgctt aaacgaaaaa ccccgcccgg
    89701 tttgcgccgg cggggttttg gaatcgtgtg ttgttccagt ccctacggcg cattgccgac
    89761 gaccaccacc acacgcacga cgaccactgc ggcggatggc gcagttggta gtagttttgc
    89821 gttgaacatg gaagtagtca ttggggacct tgttggtttt gtgtttaaca atatttatac
    89881 aagcacagct ttacagggga gacaatggaa aatttttcag caagggaaaa ttgaggggtt
    89941 gatcacgttt tgtactgaat tgcagataac aaaaaacccc gccggagcga ggtttcgtca
    90001 gtcgcctgcg gctggtaacc gcaaagcaca ctgtattatg tcaacactga aagtatacgt
    90061 gttccgcgca gaacgcgcaa tttcggcacg aattttgacg tatttagtgc atagttgagt
    90121 atcgatcaca gtttgcgttt tgtccaaata ttactgttta tttatacagt aaacttctat
    90181 aatatcactg tacgcaatgt gttatgcggg ggccgcatcg ttacccggcg cactaagtcc
    90241 tggctgaaac gggtggtgcc gtcagcgcct taaccccgcg tgagcacact gtgttatgtc
    90301 aacaagcaca acgtttctcc ttgagatacc gcgtgcacaa cagctggcaa caggcagcgg
    90361 aaaggtacgt cagctggcag tgctcctgaa ccacaggaga cgcgtatgag cctggtggat
    90421 attgccattc ttatcctcaa actcattgtt gcagcactgc aactgcttga tgctgttctg
    90481 aaatacctga agtaattcag attcaagtcg caccaaaggg gagcgggaaa ccgctcccct
    90541 tttatattta gcgtgcgggt tggtgtcgga tgcgatgctg acgcatctta tccgccctac
    90601 catctctccc ggcaacattt attgccgctt ttgtttacat attctgccgc taaacaattc
    90661 cccattcctg gcgtatatct ggctaacatt catcaatgtg atagattcct ctcccgcatt
    90721 tatgggaatg cgtagtgact tattctaatt atttttataa aagcatccgt gataatgaaa
    90781 aggcaaagaa acgtcaattt gttattgatg ttggtattac tcgtggccgt cggtcagatg
    90841 gcgcaaacca tttatattcc agctattgcc gatatggcgc gcgatctcaa cgtccgtgaa
    90901 ggggcggtgc agagcgtaat gggcgcttat ctgctgactt acggtgtctc acagctgttt
    90961 tatggcccga tttccgaccg cgtgggccgc cgaccggtga tcctcgtcgg aatgtccatt
    91021 tttatgctgg caacgctggt cgcggtcacg acctccagtt tgacggtgtt gattgccgcc
    91081 agcgcgatgc aggggatggg caccggcgtt ggcggcgtaa tggcgcgtac tttgccgcga
    91141 gatttatatg aacggacaca gttgcgccat gctaacagcc tgttaaacat ggggattctc
    91201 gtcagtccgt tgctcgcacc gctaatcggc ggtctgctgg atacgatgtg gaactggcgc
    91261 gcctgttatc tctttttgtt ggttctttgt gctggtgtga ccttcagtat ggcccgctgg
    91321 atgccggaaa cgcgtccggt cgatgcaccg cgcacgcgcc tgcttaccag ttataaaacg
    91381 cttttcggta acagcggttt taactgttat ttgctgatgc tgattggcgg tctggccggg
    91441 attgccgcct ttgaagcctg ctccggcgtg ctgatgggcg cggtgttagg gctgagcagt
    91501 atgacggtca gtattttgtt tattctgccg attccggcag cgttttttgg cgcatggttt
    91561 gccggacgtc ccaataaacg cttctccacg ttaatgtggc agtcggttat ctgctgcctg
    91621 ctggctggct tgctgatgtg gatccccgac tggtttggcg tgatgaatgt ctggacgctg
    91681 ctcgttcccg ccgcgctgtt ctttttcggt gccgggatgc tgtttccgct ggcgaccagc
    91741 ggcgcgatgg agccgttccc cttcctggcg ggcacggctg gcgcgctggt cggcggtctg
    91801 caaaacattg gttccggcgt gctggcgtcg ctctctgcga tgttgccgca aaccggtcag
    91861 ggtagcctgg ggttattgat gaccttaatg ggattgttga tcgtgctgtg ctggctgccg
    91921 ctggcgacgc
//
LOCUS       DACAPB010000002        73147 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.002, whole genome shotgun sequence.
ACCESSION   DACAPB010000002 DACAPB010000000
VERSION     DACAPB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.002"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(180..1478)
                     /gene="eno"
                     /locus_tag="IT904_000087"
     CDS             complement(180..1478)
                     /gene="eno"
                     /locus_tag="IT904_000087"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP3189928.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1566..3203)
                     /gene="pyrG"
                     /locus_tag="IT904_000088"
     CDS             complement(1566..3203)
                     /gene="pyrG"
                     /locus_tag="IT904_000088"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAP3189929.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3431..4222)
                     /gene="mazG"
                     /locus_tag="IT904_000089"
     CDS             complement(3431..4222)
                     /gene="mazG"
                     /locus_tag="IT904_000089"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAP3189930.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4293..4628)
                     /gene="mazF"
                     /locus_tag="IT904_000090"
     CDS             complement(4293..4628)
                     /gene="mazF"
                     /locus_tag="IT904_000090"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HAP3189931.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4628..4876)
                     /gene="mazE"
                     /locus_tag="IT904_000091"
     CDS             complement(4628..4876)
                     /gene="mazE"
                     /locus_tag="IT904_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HAP3189932.1"
                     /translation="MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4954..7188)
                     /locus_tag="IT904_000092"
     CDS             complement(4954..7188)
                     /locus_tag="IT904_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP3189933.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7236..8537)
                     /gene="rlmD"
                     /locus_tag="IT904_000093"
     CDS             complement(7236..8537)
                     /gene="rlmD"
                     /locus_tag="IT904_000093"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP3189934.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8594..11350
                     /gene="barA"
                     /locus_tag="IT904_000094"
     CDS             8594..11350
                     /gene="barA"
                     /locus_tag="IT904_000094"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAP3189935.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11583..12923)
                     /gene="gudD"
                     /locus_tag="IT904_000095"
     CDS             complement(11583..12923)
                     /gene="gudD"
                     /locus_tag="IT904_000095"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAP3189936.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12944..14284)
                     /locus_tag="IT904_000096"
     CDS             complement(12944..14284)
                     /locus_tag="IT904_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HAP3189937.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGDVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYVENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14286..15638)
                     /locus_tag="IT904_000097"
     CDS             complement(14286..15638)
                     /locus_tag="IT904_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3189938.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNKKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16073..16522)
                     /locus_tag="IT904_000098"
     CDS             complement(16073..16522)
                     /locus_tag="IT904_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP3189939.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16540..17322)
                     /gene="truC"
                     /locus_tag="IT904_000099"
     CDS             complement(16540..17322)
                     /gene="truC"
                     /locus_tag="IT904_000099"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAP3189940.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17322..17651)
                     /locus_tag="IT904_000100"
     CDS             complement(17322..17651)
                     /locus_tag="IT904_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAP3189941.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18273..18818)
                     /gene="syd"
                     /locus_tag="IT904_000101"
     CDS             complement(18273..18818)
                     /gene="syd"
                     /locus_tag="IT904_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAP3189942.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18886..19734
                     /gene="queF"
                     /locus_tag="IT904_000102"
     CDS             18886..19734
                     /gene="queF"
                     /locus_tag="IT904_000102"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAP3189943.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19846..21210
                     /gene="ppnN"
                     /locus_tag="IT904_000103"
     CDS             19846..21210
                     /gene="ppnN"
                     /locus_tag="IT904_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAP3189944.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21767..23056
                     /locus_tag="IT904_000104"
     CDS             21767..23056
                     /locus_tag="IT904_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP3189945.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23114..24481
                     /gene="sdaB"
                     /locus_tag="IT904_000105"
     CDS             23114..24481
                     /gene="sdaB"
                     /locus_tag="IT904_000105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAP3189946.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24503..25348
                     /gene="xni"
                     /locus_tag="IT904_000106"
     CDS             24503..25348
                     /gene="xni"
                     /locus_tag="IT904_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAP3189947.1"
                     /translation="MRGLFPISHPAVACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHDEMPALRAAFEQRGVPCWSTSGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(25403..26551)
                     /gene="fucO"
                     /locus_tag="IT904_000107"
     CDS             complement(25403..26551)
                     /gene="fucO"
                     /locus_tag="IT904_000107"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP3189948.1"
                     /translation="MANRMILNETAWFGRGTVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGVVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26579..27226)
                     /gene="fucA"
                     /locus_tag="IT904_000108"
     CDS             complement(26579..27226)
                     /gene="fucA"
                     /locus_tag="IT904_000108"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAP3189949.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27773..29089
                     /locus_tag="IT904_000109"
     CDS             27773..29089
                     /locus_tag="IT904_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose-proton symporter"
                     /protein_id="HAP3189950.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29122..30897
                     /gene="fucI"
                     /locus_tag="IT904_000110"
     CDS             29122..30897
                     /gene="fucI"
                     /locus_tag="IT904_000110"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAP3189951.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QQIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31006..32424
                     /gene="fucK"
                     /locus_tag="IT904_000111"
     CDS             31006..32424
                     /gene="fucK"
                     /locus_tag="IT904_000111"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAP3189952.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIKRLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPCRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQIDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32426..32848
                     /gene="fucU"
                     /locus_tag="IT904_000112"
     CDS             32426..32848
                     /gene="fucU"
                     /locus_tag="IT904_000112"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAP3189953.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32906..33637
                     /gene="fucR"
                     /locus_tag="IT904_000113"
     CDS             32906..33637
                     /gene="fucR"
                     /locus_tag="IT904_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAP3189954.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33681..34781)
                     /gene="rlmM"
                     /locus_tag="IT904_000114"
     CDS             complement(33681..34781)
                     /gene="rlmM"
                     /locus_tag="IT904_000114"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAP3189955.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34774..35169)
                     /locus_tag="IT904_000115"
     CDS             complement(34774..35169)
                     /locus_tag="IT904_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAP3189956.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35188..36105)
                     /locus_tag="IT904_000116"
     CDS             complement(35188..36105)
                     /locus_tag="IT904_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAP3189957.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36456..36683)
                     /locus_tag="IT904_000117"
     CDS             complement(36456..36683)
                     /locus_tag="IT904_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAP3189958.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36875..38080
                     /gene="csdA"
                     /locus_tag="IT904_000118"
     CDS             36875..38080
                     /gene="csdA"
                     /locus_tag="IT904_000118"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAP3189959.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38080..38523
                     /gene="csdE"
                     /locus_tag="IT904_000119"
     CDS             38080..38523
                     /gene="csdE"
                     /locus_tag="IT904_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAP3189960.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38574..39380)
                     /gene="tcdA"
                     /locus_tag="IT904_000120"
     CDS             complement(38574..39380)
                     /gene="tcdA"
                     /locus_tag="IT904_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAP3189961.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIE
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39619..40716)
                     /gene="mltA"
                     /locus_tag="IT904_000121"
     CDS             complement(39619..40716)
                     /gene="mltA"
                     /locus_tag="IT904_000121"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAP3189962.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40925..41001
                     /locus_tag="IT904_000122"
     tRNA            40925..41001
                     /locus_tag="IT904_000122"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40959..40961,aa:Met,seq:cat)
     gene            complement(41075..42328)
                     /locus_tag="IT904_000123"
     CDS             complement(41075..42328)
                     /locus_tag="IT904_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAP3189963.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42560..43891
                     /gene="argA"
                     /locus_tag="IT904_000124"
     CDS             42560..43891
                     /gene="argA"
                     /locus_tag="IT904_000124"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAP3189964.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43953..45779)
                     /gene="recD"
                     /locus_tag="IT904_000125"
     CDS             complement(43953..45779)
                     /gene="recD"
                     /locus_tag="IT904_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAP3189965.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLSDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(45779..49321)
                     /gene="recB"
                     /locus_tag="IT904_000126"
     CDS             complement(45779..49321)
                     /gene="recB"
                     /locus_tag="IT904_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAP3189966.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIERRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(49314..52202)
                     /gene="ptrA"
                     /locus_tag="IT904_000127"
     CDS             complement(49314..52202)
                     /gene="ptrA"
                     /locus_tag="IT904_000127"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAP3189967.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52378..55746)
                     /gene="recC"
                     /locus_tag="IT904_000128"
     CDS             complement(52378..55746)
                     /gene="recC"
                     /locus_tag="IT904_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAP3189968.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAHWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDATLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55759..56082)
                     /locus_tag="IT904_000129"
     CDS             complement(55759..56082)
                     /locus_tag="IT904_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP3189969.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56067..56474)
                     /locus_tag="IT904_000130"
     CDS             complement(56067..56474)
                     /locus_tag="IT904_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAP3189970.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(56471..57034)
                     /locus_tag="IT904_000131"
     CDS             complement(56471..57034)
                     /locus_tag="IT904_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP3189971.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(57025..57495)
                     /locus_tag="IT904_000132"
     CDS             complement(57025..57495)
                     /locus_tag="IT904_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP3189972.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57680..58474)
                     /gene="thyA"
                     /locus_tag="IT904_000133"
     CDS             complement(57680..58474)
                     /gene="thyA"
                     /locus_tag="IT904_000133"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP3189973.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58481..59356)
                     /gene="lgt"
                     /locus_tag="IT904_000134"
     CDS             complement(58481..59356)
                     /gene="lgt"
                     /locus_tag="IT904_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP3189974.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59507..61753)
                     /gene="ptsP"
                     /locus_tag="IT904_000135"
     CDS             complement(59507..61753)
                     /gene="ptsP"
                     /locus_tag="IT904_000135"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP3189975.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61766..62296)
                     /gene="rppH"
                     /locus_tag="IT904_000136"
     CDS             complement(61766..62296)
                     /gene="rppH"
                     /locus_tag="IT904_000136"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAP3189976.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            62981..63670
                     /gene="mutH"
                     /locus_tag="IT904_000137"
     CDS             62981..63670
                     /gene="mutH"
                     /locus_tag="IT904_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAP3189977.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63739..64452
                     /locus_tag="IT904_000138"
     CDS             63739..64452
                     /locus_tag="IT904_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP3189978.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64590..64808
                     /gene="ygdR"
                     /locus_tag="IT904_000139"
     CDS             64590..64808
                     /gene="ygdR"
                     /locus_tag="IT904_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAP3189979.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            64916..65956
                     /locus_tag="IT904_000140"
     CDS             64916..65956
                     /locus_tag="IT904_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAP3189980.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGIELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(65988..67181)
                     /gene="lplT"
                     /locus_tag="IT904_000141"
     CDS             complement(65988..67181)
                     /gene="lplT"
                     /locus_tag="IT904_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAP3189981.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(67174..69333)
                     /gene="aas"
                     /locus_tag="IT904_000142"
     CDS             complement(67174..69333)
                     /gene="aas"
                     /locus_tag="IT904_000142"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAP3189982.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            69918..70949
                     /gene="galR"
                     /locus_tag="IT904_000143"
     CDS             69918..70949
                     /gene="galR"
                     /locus_tag="IT904_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAP3189983.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(70956..72218)
                     /gene="lysA"
                     /locus_tag="IT904_000144"
     CDS             complement(70956..72218)
                     /gene="lysA"
                     /locus_tag="IT904_000144"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP3189984.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            72340..>73147
                     /gene="lysR"
                     /locus_tag="IT904_000145"
     CDS             72340..>73147
                     /gene="lysR"
                     /locus_tag="IT904_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAP3189985.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTV"
BASE COUNT        17734 a        19747 c        17990 g        17676 t
ORIGIN      
        1 agagcatctg gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcagatagc
       61 ggcggattta gcggcggggg cggttcttct ggaggtggcg gcgcatccgg ccgctggtaa
      121 ctcaaccata aaaaatgcca gcccggaggc tggcattttt aaatcagata aagtcagtct
      181 tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc cagagcttct
      241 tcgatacgaa tcagctggtt gtatttagca acacggtcag aacggctcat agaaccagtt
      301 ttgatctggc ctgcagcagt accaacagcc aggtcagcga tggtagcgtc ttcagtttcg
      361 ccagaacggt gagagataac tgcagtgtag ccagcatctt tcgccatctt gattgcagcc
      421 agagtttcgg tcagagaacc gatctggttg aatttgatca ggatggagtt agcgatacct
      481 ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc accaaccagc
      541 tggattttgt cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc
      601 agaccgtctt cgatagaaac gatcgggtac tgtttggtca gttcttccag gaagtgagtg
      661 aattcttcag aggtgaacgc tttgttgcct tcgccagcca gaacgtattt accatctttg
      721 tagaattcag aagctgcgca gtccatcgcc aaagtgatgt ctttgcccag ttcataacca
      781 gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc caggttcggc
      841 gcatagccac cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag aacttttgcc
      901 aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt cgcgccaacc
      961 ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc accgttgatg
     1021 atgttcatca tcggaaccgg catagagtat ttgcccggag taccgttcag ttcagcgatg
     1081 tgctcgtaca gcggcatacc tttagcagct gcagcagctt tggcgttagc cagagataca
     1141 gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag gtcgatcatg
     1201 atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg agcgatcggg
     1261 ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga tttgtcgcca
     1321 tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc agctgccata
     1381 ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc acgggagtcg
     1441 atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc aagtcactag
     1501 ttaaactgaa actccagaca aacaacgcgt acccagggta cgcgttgccg ctctaacttt
     1561 tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac
     1621 agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca agccacgaac
     1681 cacgggtgat tcggaacttc gatgatctcg accaactgat catccccgga acggcccgca
     1741 acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc gtaacggtga
     1801 cgatgacgct caacaattgt cggcgcattg tacagctggc gaaccaggct atcgtcaacc
     1861 aactggcact gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt ctcgctacga
     1921 acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac cgggtactta
     1981 cagtctggca caaattccgt agagttggcg ttctccatgt tggcaacatg gcgagcgtaa
     2041 tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt gttctcacgc
     2101 gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccgaaacc gccaggtacg
     2161 aggattgcgt ccagaccttt aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg
     2221 atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag tgcttcgatc
     2281 actgatttat aagcatccgg cagttcaatg tacttgccga ccataccgat ggtgacttca
     2341 cttaccgggt tcgcttcttc gaagataacc tgttcccatt cggacagatt cgcttccggg
     2401 cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt caacaggccc
     2461 ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc cggaacatta
     2521 cagaacaatg caatcttcgc acgttcgttc gccggaacag cgcgatctga acgacaaatc
     2581 aggatgtcag gctggatacc gatggagagc agctctttta cagagtgctg agtcggtttg
     2641 gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg
     2701 tgctcacggc caatttcaac agccatctgg cgaatcgctt cgaggaacgg caaggattcg
     2761 atatcaccta ctgtaccgcc gatttctacc agtactacgt catgaccttc gccaccttcc
     2821 agcacgcgct ctttgattgc gttagtgatg tgcggaataa cctgcacggt tgcgccgagg
     2881 tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgacccgt ggtgaagttg
     2941 ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt
     3001 tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat agtacctgga
     3061 tcgacgttga tgtacggatc cagtttcatg atggtcacat tgaggccacg ggcttcaaga
     3121 atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac cccgccggtc
     3181 acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa gacgatggaa
     3241 taaccaggac gggaaagcag tatacccgaa catgacctgt gccacaaact ttcattatcc
     3301 ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct ttggggtaaa
     3361 tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg
     3421 cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact tcttccattg
     3481 tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc tccacttcgc
     3541 gaaaacgacg ctcgaatttt tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta
     3601 aatggcgagc cagattaacc gtggcaaaca gcaggtcccc catttcctcc tccagtttag
     3661 cctggtcgac aacagcctgc cgcgcttcgt acatcacctc gtcgatctct tcgtagactt
     3721 tatcgactac cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa cgtttctgga
     3781 ttttttgcgc acgcattaaa gccggtaaac tacgaggaat atcgtccagc gccgaatgct
     3841 gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc acttcactac
     3901 tgttttcggc agaactgtca gcaaaaacat gcggatggcg acgctctaat ttatcgctaa
     3961 tcgcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa
     4021 acaccacctg gaacagcaga tcgccgagtt caccgcgcag atcgtcaaaa tcttcacggg
     4081 cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg gtggcaaatg
     4141 tctgctcttt atcccacggg cagccgtttt ccggatcgcg caggcgctgc ataatagtga
     4201 gcaaacggtc gatttgattc attgaattgt cctgaaaatt gcgggtctgt caggtggaaa
     4261 cctgtgacca gaatagaagt gagttagtaa cactacccaa tcagtacgtt aattttggct
     4321 ttaatgagtt gtaattcctc tggggcaact gttcctttct tcgttgctcc tcttgcccgc
     4381 caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg accggataaa
     4441 acaacttcga acggatatcc ttttgattgc gttgtacaag gaacacacag acacatacct
     4501 gttttgttgt tgtacatgaa aggactcagg acaacagctg gacgatgtcc agcttgctcg
     4561 ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg tacgtatcgg
     4621 cttaccatta ccagacttcc ttatctttcg gctctcccca gtcgatattc tcgtggaggt
     4681 tttccggcgt gatgtcgttg accagttcag caagcgtaaa tacgggctct ttacgcactg
     4741 gctcaataat taatttgcca tccaccaggt caatcttcac ttcatcatca atattcagat
     4801 tgagcgcctg cattaacgta gtcgggatcc gcaccgccgg tgaatttccc caacgcttta
     4861 cgctactgtg gatcataacc ctttcctcaa accgctatca tatgtagata cagtatatat
     4921 caatctacat tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc gacgcgcgtc
     4981 gataacatcc ggcacctggt tgagtttacc cagcacgcgc cccagcactt gcaggttgta
     5041 aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac
     5101 gccaagcacg ttcaccttct cgttggcgag aatggtcgtg atatcacgta acaacccact
     5161 acgatcatta gctaccacgc ggaccaccag cgaatatccg gcggagtagc tctcacccca
     5221 taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt gttcgcaatc
     5281 ggcgcggtgt actgaaatac cgcgcccctg ggtaatgaag ccgacaatct catctccagg
     5341 aatcggctgg cagcagcgcg cgatgtggtg catcaggttg ccaacacctt cgactaccac
     5401 gcgaccgtta tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag
     5461 cgcggcggcg tcctgctctt cggcactcgg cttattaaat tgcgattgca ggaagttcac
     5521 catctgattg agacggatat ccccgccacc aatcgccgcc agcaactcgt cgacatcatt
     5581 gaagttgtaa cgcggcagca gatgtttttc tgcttctttc aggctgatcc ccagatgttc
     5641 cagctcgtcg tcaaggattt gccgcccagc cagaatgttt ttgtcacggt cctgtttacg
     5701 gaaccaggcg tgaattttcg aacgcccacg gctggttgtg acgtaaccga ggtttgggtt
     5761 taaccagtca cggctggggt tcggctgttt ctgggtgata atttcaatct ggtcgcccat
     5821 ctgcagctgg taggtgaacg gcacaatgcg cccgccaatt tttgccccaa tgcagcggtg
     5881 tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc
     5941 aacgacatca cctttcggcg taaagacgta cactcggtcg tcaaagacct ggctgcgtac
     6001 ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc catgcaatca gtttacgcag
     6061 ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca gcagccgcgc cctctttata
     6121 tttccagtgc gcagcaacac ccaactctgc atcttcatgc atctgtttgg tgcggatttg
     6181 gatctcaacg gttttgccac ctggccccag aaccacggta tgaatagact gataaccgtt
     6241 tggtttcggg ttagcgacgt aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg
     6301 cactatcccc agtgcggcat aacaatcctg taaacgctcg gcgacaatac gtaccgcacg
     6361 cacatcaaac agctcatcaa aggcgaggtt ctttttctgc attttacgcc agatgctgta
     6421 gatgtgtttc ggacgaccat acacttccgc tttaacgcct tcagctttca tctcagcgcg
     6481 cagatgacca acgaactctt cgatgtagtg ttcgcggtcg agacgccgtt catgcagcag
     6541 tttggcaatt cgtttgtatt cggttggatg gaggtaacgg aagcagtaat cttccagttc
     6601 ccatttcagt tgtccgattc cgagacggtt agccagcggt gcgtagatgt tggtacactc
     6661 ttttgccgcc agtacacgtt catcttccgg cgcatctttt acttcgcgca gatgagcaat
     6721 acgctccgcc agtttgatga ctacgcagcg aaaatcatcg accatcgcca ataacatccg
     6781 gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg
     6841 gatcgccgcc atatcacgca cgccgtgaat aaggttaacg accgacttac cgacgctctc
     6901 acgcagcaca tcttcgctga ctacgttggc atccgccaga gggaaaagca gcgccgcccg
     6961 cagcgtgtca atgtccatac ttaatgtcga gaggatctcc accatctcaa caccacgcca
     7021 caataacaga ctggcatccg gatgcccctg cgtctgttgc agacaatacg cccaggtttc
     7081 ggctaagcac tcacacgact tctggctggt aatacccaga cttgcgatcc atttttccgg
     7141 atcaaattca ccagccttat tgatatgtgc acttcttacc gcaaccatcg tcctctcctt
     7201 tagggaccag acctgccgaa atcggcaaat cgcaactatt taacgcgcga gaaaagtacc
     7261 atcgattcca gatgtcccgt gtgtgggaac atatccagca tcgccagtcg cgcaatggta
     7321 tatcctgctt ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt acaggataca
     7381 taaactatac gaataggttc cagttttata atttgctgca taacacctgc ggcacctgct
     7441 cgcgccgggt ccagcaacac tttatcgaag ccgtttttcg cccacggctg ctttgtgaca
     7501 tcttcttcaa gattttcatg ataaaacgtc acattctgta agccattaag acgcgcattc
     7561 tgctggcctt tttccaccag cgccggaaca ccttctacac ccaccacact ggcagcttgt
     7621 gtcgccaatg gcagtgtaaa gttgcccata ccgcagaaca gatccagtac gcgatcttca
     7681 ggttgcacat ccagccattc caacgcacgc gctaccattt tttggttcac acccgcattg
     7741 acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc ataccagggc
     7801 atctcaccag agacggtttc gagtatctca ctatcggggg cgagatacag atccaggcct
     7861 tcagaatgcg aaaagcgttc cagtttttcg cgatctgccg aacttagcgg tgcggtatgg
     7921 cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc aagatggcgc
     7981 atagcttgta agctgcccag acatgccctg actttgggca gcaatgcttc aagttggggc
     8041 gctaaaatgg ggcattgttt gacgtcgaca atgtcactgg agcccgcttt gcgaaacccc
     8101 atctgaagtt gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg acggcgatag
     8161 ccccagggaa catcggcgat cacttcagag acatcgtgtt tcattaatcg ggcgagtgcc
     8221 gcacttttgc ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca gccaccgcat
     8281 acgccaaaat gaggacagcg tggcgtttcg cgttccgggc tatcgcttaa ccggcgtacg
     8341 actttagcgc gggcatactg ttttttatct tcagtaacag taacttccgc gttttcctgc
     8401 ggcaataatc cggggataaa tagcgttttg ccgttatgtc gcgccacgcc ctgaccaaaa
     8461 gagtcgaggt cgttgactga aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca
     8521 gagtagaatt gcgccattgg cgagactttc tcaatttaac agtgtgacct taattgtccc
     8581 ataacggaac tccatgacca actacagcct gcgcgcacgc atgatgattc tgatcctggc
     8641 accgaccgtc cttattggtt tattgctgag tatctttttc gtcgtgcatc gctataacga
     8701 cttgcagcgt caactggaag atgccggtgc cagcattatt gagccgcttg cagtttctac
     8761 tgaatatggc atgagcctgc aaaatcgcga atctatcggt cagttaataa gcgtactgca
     8821 tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat gatgaaaata accgactctt
     8881 tgtcacctcc aattttcatc ttgatccctc atcaatgcag ctcggcagca acgtgccgtt
     8941 tcctcgccag ctcactgtca ctcgtgacgg cgatattatg atcctccgca cgccgattat
     9001 ttctgagagt tactcccccg acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat
     9061 gttgggatat attgcgctgg agctggatct taaatcggtt cgcttgcagc aatataaaga
     9121 gatctttatt tccagcgtga tgatgctgtt ttgtatcggt attgcgctta tttttggctg
     9181 gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat
     9241 ccgtcgcggg caactcgaca gccgggtgga aggatttatg ctcggcgagc tggatatgct
     9301 gaaaaacggt atcaactcga tggcaatgtc gctggctgct tatcacgaag agatgcagca
     9361 caatatcgac caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa
     9421 cgttgagtta gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt
     9481 tctggcaaat atgtcacacg agctgcgtac accactgaat ggtgttattg gctttacccg
     9541 cctgacgctg aaaacagaat taacaccaac gcagcgcgat cacctgaata cgattgaacg
     9601 ttcggcaaat aatttgctgg caattattaa tgatgttctc gacttctcga aactggaagc
     9661 aggtaagctg attctggaaa gtattccatt cccactacgc agcacgctgg atgaagtcgt
     9721 tactctgctg gcacattctt ctcacgataa agggttagaa ctgacgctca atattaaaag
     9781 cgacgtgcct gataacgtga tcggcgaccc gctgcgatta cagcaaatca tcactaacct
     9841 ggtggggaat gcaattaaat tcaccgagaa tggcaacatt gatattctgg tagaaaaacg
     9901 tgcgctgagt aataccaaag tgcagattga agtgcagatt cgggataccg gcattggtat
     9961 tcctgaacgc gatcaatcgc gcttattcca ggccttccga caggctgatg ccagtatttc
    10021 ccgccgtcat ggtggcaccg ggctggggct ggtgattaca caaaaactgg ttaatgaaat
    10081 gggcggcgat atttcgttcc atagccagcc gaatcgcggt tcaactttct ggttccacat
    10141 taatctcgat ctgaacccga acattattat cgaagggcca tccacccagt gcctcgcagg
    10201 taagcgcctg gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc tggatatttt
    10261 aagtgaaacg ccgctggaag tggtttatag cccaacgttc tccgcgctgc ctcccgcgca
    10321 ttacgacatg atgttgttag gcatcgcggt gaccttccgc gagccgctaa caatgcaaca
    10381 tgagcgatta gcgaaagcgg tatcgatgac cgatttcctg atgctggcac ttccttgcca
    10441 tgcacaagtc aatgctgaaa aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc
    10501 attaacacct acacgcctgt tgcctgccct gacggaattt tgtcatcaca aacaaaacac
    10561 gcttttgcct gtaaccgatg aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa
    10621 ccccgctaac ctgaaactta tcggcgcatt gctggaagat atggtgcaac atgtggaact
    10681 ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag atgccgttcg atttgatctt
    10741 aatggatatt caaatgccgg acatggatgg cattcgggcc tgcgaactca tccaccaact
    10801 cccgcatcag caacaaacgc cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa
    10861 agagaagctg cttggcgcag ggatgagcga ttatctggcg aaaccgattg aagaagagcg
    10921 attgcataat ttgttgttgc gctacaagcc tggcagcggt atttcctctc gcgtcgtgac
    10981 gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc aactggcact
    11041 acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt
    11101 cctgcctgaa gttcgcaaca aagttgagga acagctggtt ggagaaaacc cggaaggcct
    11161 ggttgatttg attcataaac tgcatggcag ttgcggctat agcggtgtgc cacgtatgaa
    11221 gaatctctgc caactgatcg aacaacagct acgtagcggt actaaagaag aagatttgga
    11281 accggagctg ctggaactgt tggacgagat ggataatgtc gcgcgcgaag ccagcaaaat
    11341 tctcgggtaa tggatgtctt gtcgagtagc aatcggcttt ttatgacgct ggtttcagat
    11401 tggtgacaaa gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc cggcctacaa
    11461 aaccaattaa attcaataaa ttaaaaagca tgtaggcctg ataagcgtag cgcatcaggc
    11521 aattttgtgt ttggcatcag tctcaaaccg gctcctgaca gagccggttt tggttttctg
    11581 tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc agccaggaat cagatactgc
    11641 attcccatcg catcgtcacg cgcgccaagc ccgtgtttct gatacagctc atgggctttc
    11701 atcacttgat ccatatcgat ttctacaccc agccccggtt tttctggcac ctgtaccagc
    11761 ccgcctttga tctcaaacgg ttctttggtc aggcgctgat taccttcctg ccagatccag
    11821 tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg caacatgggt aaacatcgcc
    11881 agggaaatat cgaagtggtt gttagagtgt gaaccccagg tcaggccaaa ttcatggcac
    11941 atttgcgcca cacgtaccga accttgcatt gtccagaaat gcggatccgc cagcgggata
    12001 tcaacggatt gcagggagag cgtatggccc atttgccgcc agtcggtggc gatcatattg
    12061 gttgcagtcg gtagacctgt cgcgcgacgg aactctgcca tcacttcacg cccggagaaa
    12121 ccttgctccg caccacacgg atcttctgca taagccagcg aacctttcag gtatttaccg
    12181 attttaatcg cttcgttcag cgaccaggca ccgttaggat cgagcgtaat acgcgcctgc
    12241 gggaagcgtt gcgccagtgc cacaatagac tcggcctctt cttccccggc cagtacaccg
    12301 cccttcagtt tgaaatcgtt gaagccatat ttttcatatg ccgcttccgc caggcgcacc
    12361 accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat accagtcgca tgagtcatcc
    12421 ggctggcttt gatacggcag cggcgtggct ttgcgattac cgacgaagaa cagataaccg
    12481 agcatttcga cttcgctacg ctgttgacca tcgcccagca gcgatgccac gtttaccccc
    12541 agatgctgcc ccagcagatc cagcattgcc gcttctatcc cggtaactac atgaatagtg
    12601 gtacgtaggt caaatgtctg caaaccgcgc ccaccagcat cacgatcggc aaaagtatta
    12661 cgcaccagcg tcagaacgtt tttgtattca cccagcgttt tacctaccac cagcggaatc
    12721 gcatcttcca gcgttttacg gattttctcg ccgccgggaa tttcccctac gccagtgtgg
    12781 ccagaattat ctttgataat cacaatatta cgcgtaaaga acggtgcgtg tgcaccactc
    12841 agattcatca gcatactgtc atgacccgcc accgggataa cctgcatttc agtaacaaca
    12901 ggcgtcgtaa attgagaact catatttatg tcctttttca gaatcaatga cggccgaaaa
    12961 cgggacgttt acggtcaaag gtccagccgg ggatcaggta ctgcatcgga cctgcgtcgt
    13021 tacgcgcacc gccaggcaga cgtttatagg cctcatgtgc cttttgtacc tgttcccagt
    13081 ccagttccac gcccagaccg ggcgcatcag gaacggcaat ttttccgttt ttaatctcca
    13141 gcggattttg ggtcaggcga caatcgccct cctgccaaat ccagtgggta tcgatagcgg
    13201 taggattacc cggtgccgcc gcgcccacat gggtaaacat cgccagagag atatcgaaat
    13261 ggttattaga atggcagccc caggtcagcc cccagtcgtc gcaaagctgc gccacacgga
    13321 ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg aatatctacc gcattgagca
    13381 tcaccgcatg acccatttcg cgccagttgg tggcgatcat gttagtcgcg acgggcaagc
    13441 cggtcgcccg tcgaaattcc gccatcactt cacgtccgga gaagccctgt tctgcgccgc
    13501 atggatcttc ggcataggta agaacatcat tcagcccttt gcataaagaa atggcttcat
    13561 caagcagcca tgcaccgttg ggatcaacgg taatccgcgc atccgggaag cgtttcttca
    13621 atgcacgaac agtgtcgatt tcttgctcgc caggtaacac gccgccctta agtttgaaat
    13681 ctttaaagcc gtagcgatcc tgtgaggctt ccgccagacg cacaacggct tcgctgttca
    13741 ttgctttctg atgccgcaac tgataccact catggttgcc cggcgtattt tccacataag
    13801 gaagatcggt tttggtccga tcaccgatat aaaacagata accgaggacg gtaatagcct
    13861 cgcgttgctt gcctggccct aacagttcgc agaccggaac attcagcgcc ttacctagca
    13921 ggtcaagcaa agcggcttcc agcgccgcca cggcgttaac gcgtaattca aaggtccagg
    13981 cacctttacc gaaggtatca aaatcggcgg cctgattacc tttatgcacc tgctggacca
    14041 ctttattcag tcgcgcaact tcctggccca gaaccatcgg aatagcatcg acaagcgtct
    14101 gataaatcac atctccgccc ggcgcttcac caatgccggt atgcccggcg ttatcggtga
    14161 gtaccacaat attgcgggtg aaatatgcgt tatgtgcccc accaatatta agcaacatgc
    14221 tgtcatgccc ggccaccgga atgactttca tatcagtaat aacaggactg gattgtgtcg
    14281 ccatcttatt gccccgcaac aggtttcaac tcgatacgct tgatatcgcc caccagcacc
    14341 aggtagctca gtaccgcgat taaggcatga acaccaacat aaatcagcgc cccattaaac
    14401 gagccagtcg tgccaacgat ataaccaatt gcgattggcg tgacgatgcc agaaatgtta
    14461 ccgaacatgt tgaacaggcc accggaaaga ccgctgatct cttttggcgc ggtatctgcc
    14521 attactgccc aacccagcgc cccgatgccc ttaccgaaga aggccagcgc cataaagccg
    14581 atgatcatcc actcaacgtt gacgtagttg cagaacacca tcaccatcga cagcaacatg
    14641 cccattacga tcggtgtttt acgcgcaatg ttcagcgatc ccgtgcggcg catcagccag
    14701 tcggaaataa tcccacccag cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc
    14761 acaaagcccg ctttcagaat cgacatcccg cgtgcctgca ccagataaac cgggaaccag
    14821 gtaataaaga agtaagtcag ggcgttgata cagtactgac cgatataaac gccgatcatc
    14881 atccgtgacc ctagcagctg tttgatctgc ccccacttca cgctgaacgg cactttaact
    14941 ttggtgtttt gctgatccat attgatcagc gcaccacccg cggcgatgta ctccagctct
    15001 ttcttattta cccccggatg ttgatttggc tcgtggatga ctttcaacca gataaagctg
    15061 atgacaatcc ccagaccgcc cataaagaag aagacgtgtg accagcccac ttcatgcgtc
    15121 agccagccca taatcggcgc gaagatcacc gttgcgaagt attgagcgga gttaaaaatc
    15181 gacaccgccg ttcccctttc ctgcgccgga aaccaggccg caacaatgcg actgttgccg
    15241 gggaaagatg gcgcttcagc aagcccgacc aggaagcgca gcgtaaacag ggcaacgata
    15301 atgccgaatc cactaaagat atcgacgaag ccttgcagca aggtaaacat cgaccagata
    15361 aagatcgacc agaagtagac gcgttttgaa ccaaaacggt ccagcaacca gccaccaggg
    15421 atctgcccga taacataagc ccatgagaaa gcagagaaca catagcccat tcccacggga
    15481 tcaaggccga tatctttggc catttccgaa ccggcgatag agagcgtagc gcggtcgccg
    15541 tagttgaagg atgtgacgat aaacaacatc accactatcc agtaacgagc atttgtgcgt
    15601 ttttccacac tgctcgcagc ctgacttaaa gaactcattg ttgcactcct gaaaattcgc
    15661 gttagccacg ctcactctgg actgcgacat cgccaggaaa tcagaggtga cgtagggtgt
    15721 tttttgccgt ttttataggt cgttcgccga atacggcgca tgtttatatc tggcaatagc
    15781 agtataaaaa gcgcgccata gcggctcacc gtgcaacaac acaacattaa tgcgttcaat
    15841 gaggcccgat tttggcatta gccctggacg gtggaatcca cttcacggaa atgaaaacaa
    15901 gaacaagaaa ggaagggtta aaacgaagaa ataaagaaga gtatgaaatg gatcgcttga
    15961 ctccaggcaa acgccagtaa aaatccgcgc tatgaagcag tttttactgg catttgcctg
    16021 aaaagattcg attcagcact gctaaaacga catttaccgc tcgctgaaca tatcaggaca
    16081 acagcgtgcc ccactgttcg acccacggat ttgattccgt ttccggttcc gggttctcac
    16141 tggcgtcaat caacagcatt tcgccaaccc gctgagcgct ctgttcctgc aacaaggcat
    16201 cgaactgttt gccgccattg cagaaattca cataactact gtcgccgagc gcaatcacgc
    16261 cataacgcag attcggctgg aagcccagac tatctttgat tccctgaaag agtggcacaa
    16321 tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac caccagaaca tacttatcct
    16381 gatagggcag ccagtcgctt aattcaggat cttcaaatac cgttgctttg tggccctgcg
    16441 cggtcagaat cgcttccgct tcttcggcca ctaacagtga attcccgtac atggtgccga
    16501 caaaaatacc aatttccgcc ataccgtttt ctccctggat taggaactta tctctccatc
    16561 ctgacccgat ggcgcactga actcaaccct ttcattttca ggaagcagac cgcgccagcc
    16621 aaattgtgat aacgcctgca tccaggtgtc gtccaggccc gcgtgaatag tcagcggctc
    16681 gccagtaaaa ggatgcgtca atgacaactg actggcatgt agcattaacc gctggaggcc
    16741 aaaatgctca gcaccgctgc gattctggcg taaatcgcca tgtttgctat cgccaataat
    16801 cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt ttgcgtccgg ttttcggctc
    16861 cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg gtcgctaccg gcatttctac
    16921 ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg cctttatctt cgcgggcaaa
    16981 tttatcagcg attttgtcca gttcttccac cagtggataa tccagcaccg cttcttccat
    17041 caaccagccg cgcacaatcg catggtaacg tttctggatt tggtgctgtt caaactgttg
    17101 tgccagcagc cgtccggcct cgctggataa tcccatcaac aacacaccag aagtgggtcg
    17161 gtccagacga tgagcagtaa aaacatgctg gcctatctgg tcacgcacgg tttgcatgac
    17221 cactactttc tcgtcgcgat ccagccagct gcggtgaacc agccagccgg agggtttatt
    17281 taccgcaacc agccattcat cctgatagag tatttccagc attagctcgc atcatccgca
    17341 aaaagagcat ccagtttttc cagctcagcc agaataagcg cgcgttgcgg atggtccgtc
    17401 gccagcgcca tttcataata gggtgcaacg gcaaaagcgc ccggtaacgg ctgtttgtta
    17461 tctaacaaat cgtgcattcg cgggatcagc acccactgca accactccag tggttccatg
    17521 gtgtccataa agaacggttg ggtactatta aattgatgcg gctggggttc atcgtttcgc
    17581 cagtgctgat gttcacgcag taacgcttca agcgcctgca actggagacg aacgcggtca
    17641 tgagtggtca taaagcaacc tcaataagaa aaactgccgc gaaggatagc aggaataaaa
    17701 aaagggagca ctgtattcac agcgctcccg gttcgtttcg cagcattcca gctacttttg
    17761 ttgctccctg ctcatccttg acaacttttc ctctgtcctt gcggccaatc gttcatcctg
    17821 aactattgct tcctgctcac accaccccga tgtgatactt catcctgaag tgtccctggc
    17881 cttcctgacc caccgaatca tcctgaccgg ttctcattct ccatcctgga ggtgtccttt
    17941 aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc tttccctgaa acaccatcct
    18001 ggtgtgtcct gcagaagtgt catcatcctg atgttcactt cgttgtctga ctccctgtcg
    18061 acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc gcttacagac aatgccttaa
    18121 ggaaaatttc atatgaaagt taagaattaa caatcaacat tttgatttta aatgaaaata
    18181 acgattctta tctttttaag tattcgctta tttcctggcg atctcctaca gagtctgtaa
    18241 gagatctctc acaaccagat ggggtatttg gattacagaa gaggcttaag ttgattaagg
    18301 aattccgcaa gatttgaggc taaatgcgtc cgtttacgtg ttcccagcgt ctctttgcac
    18361 acttcaccgg agagattaca caccgagatg acctctaact cttcttccag cgtcgcgata
    18421 aatagtgtgg gcggcagttt tagccttttc tgagtcacca gatgaccaat cagattttcc
    18481 tgcacccgac ggaagtcgtc ttcactccag gtttgcagca atgtcagttt gatatcgcca
    18541 aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa aggtatgaat tgtgggttgt
    18601 atcacaatat caaaagcgcg ttcaaccgcg tttacatttt gttccccggt aaacggctgg
    18661 ggttgccagt atacggcatc ttcggtagtt gaaataatgc acggtgaagg aacaccgtac
    18721 agttcctcac ttaacggcca gcttttatgc tcttcatgcc acgcatcgca gtagcgtgca
    18781 gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta atttctctct tcacttaaac
    18841 cagatacact tgtcgtttag tttatctggt ttatgacggt gaaacatgtc ttcttatgca
    18901 aaccatcagg cacttgcggg cctgactctt ggaaaatcaa ccgattaccg ggatacctat
    18961 gacgccagcc tactgcaagg cgttccacgc agcctgaatc gcgacccgct gggtctgaaa
    19021 gcggataacc tgccttttca cggtacggat atctggacgc tgtatgaact ttcctggctg
    19081 aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg aacttgatta caccagcgta
    19141 aatctgattg agtcgaagag ttttaagctc tatctcaaca gttttaacca gacgcgtttt
    19201 aataactggg atgaggtgcg ccagacgctg gagcgcgact taagcacttg cgctcagggt
    19261 aagattagcg tggcgttata tcgtcttgat gaactggaag gccagccgat aggtcatttt
    19321 aatggcactt gcattgatga ccaggatatc actatcgata actatgaatt cactactgac
    19381 tatctggaga atgccacctg tggtgaaaaa gtagtggaag agacgcttgt cagccacctg
    19441 ctgaaatcaa actgcctgat cacccatcaa ccagattggg gttcgatcca aattcagtat
    19501 cgtggacgcc aaattgacag agaaaaactg ctgcgttacc tggtctcatt ccgtcatcac
    19561 aacgagttcc acgaacagtg cgtggaacgc atctttaatg acctgttacg cttctgccag
    19621 ccagaaaaat tgagcgttta cgcacgttat acccgtcgtg gcggtctgga cattaacccg
    19681 tggcgcagta atagcgattt tgtcccatcg accacaagac tggttcggca ataaattttt
    19741 tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa aggtcatcag gcagggctat
    19801 tgtaatcaaa gggaatgacg atattcgtcc cataaggagt ttttcttgat tacacatatt
    19861 agcccgcttg gctccatgga tatgttgtcg cagctggaag tggatatgct taaacgcacc
    19921 gccagcagcg acctctatca actgtttcgc aactgttcac ttgccgtact gaactccggt
    19981 agtttgaccg ataacagcaa agaattgctg tctcgttttg aaaatttcga tattaacgtc
    20041 ttgcgccgtg aacgcggcgt aaagctggaa ctgattaatc ccccggaaga ggcttttgtc
    20101 gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg cggtcctgcg tgacattctc
    20161 ttcgtttacg ggcaaatcca taacaccgtt cgttttccca acctgaatct cgacaactcc
    20221 gtccacatca ctaacctggt cttttccatc ttgcgtaacg ctcgcgcgct gcatgtgggt
    20281 gaagcgccaa atatggtggt ctgctggggc ggtcactcaa ttaacgaaaa cgagtatttg
    20341 tatgcccgtc gcgtcggaaa ccagctgggc ctgcgtgagc tgaatatctg caccggctgt
    20401 ggtccgggag cgatggaagc gccgatgaaa ggtgctgcgg tcggacacgc gcagcagcgt
    20461 tacaaagaca gtcgttttat tggtatgaca gagccgtcga ttatcgccgc tgaaccgcct
    20521 aacccgctgg tcaacgaatt gatcatcatg ccagatatcg aaaaacgtct ggaagcgttt
    20581 gtccgtatcg ctcacggtat cattatcttc cctggcggtg tgggtacggc agaagagttg
    20641 ctctatttgc tgggaatttt aatgaacccg gccaacaaag atcaggtttt accattgatc
    20701 ctcaccggcc cgaaagagag cgccgactac ttccgcgtac tggacgagtt tgtcgtgcat
    20761 acgctgggtg aaaacgcgcg ccgccattac cgcatcatca ttgatgacgc cgctgaagtc
    20821 gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa atcgccgtga tacaggcgat
    20881 gcctacagct ttaactggtc aatgcgcatt gcgccagatt tgcaaatgcc gtttgagccg
    20941 tctcacgaga atatggctaa tctgaagctt tacccggatc aacctgttga agtgctggct
    21001 gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta acgtaaaaga agtcggtatt
    21061 cgcgccattg aagagtttgg tccttacaaa atcaacggcg ataaagagat tatgcgtcgt
    21121 atggacgacc tgctacaggg ttttgttgcc cagcatcgta tgaagttgcc aggctcagcc
    21181 tacatccctt gctacgaaat ctgcacgtaa tctccgctcc cccggtgacg tttgttgccg
    21241 ggttttctcg tttttggtca cttactcatc aactcatttc atttgttata tgaatgtttc
    21301 ttaccaccct cacgcgacaa atatcatcac agttaatatg tcatacaatt tatgttgcaa
    21361 cgcaaacgtt tccctatttt cataaaccgt attttatcgc tatgaaaaag aaattatcgc
    21421 catgattaac taaaagtatt gattttttca gttcaaccta catatattgc gcgccccgga
    21481 agaagtcaga tgtcgtttaa tgggcaaata ttgcccttaa attctctttt acttttgatt
    21541 tacagagtaa agcgttggga taatctatct tccaagtaga ttattgtatt tgagatcaag
    21601 atcactgata gatacataac ttgtgtgtat ctttccgccc tcaaattatt acggcggtaa
    21661 atgattaagc catcgccgat agacagattt catttttacg gtcaggcacc ttcccgggct
    21721 gaactggcta aaagctgaat tatttgcatt cctccaggag aaatagatgg aaacgactca
    21781 aaccagcacg attgcgtcga aagactctcg tagtgcctgg cgcaagacag acaccatgtg
    21841 gatgttgggc ctttacggca cggcaatcgg cgcgggcgtg ctgttcctgc caatcaacgc
    21901 cggtgttggc ggtatgatcc cgctgatcat catggctatc cttgcgttcc cgatgacatt
    21961 ttttgcacac cgcggcctga ctcgcttcgt actgtctggt aaaaacccgg gcgaagacat
    22021 caccgaggtt gtagaagaac actttggtat tggcgcaggt aaactgatta ccctgctcta
    22081 tttcttcgct atctacccga tcctgctggt ttatagcgta gcaatcacca atacagttga
    22141 aagcttcatg tctcaccagc tgggtatgac accaccgccg cgtgcgattc tgtcgctgat
    22201 cctgatcgtg ggtatgatga ccatcgttcg cttcggtgag cagatgatcg ttaaagcgat
    22261 gagtattctg gtattcccgt ttgttggcgt actgatgctg ctggctctgt acctgatccc
    22321 gcagtggaac ggcgcagcac tggaaacgct gtctctggac actgcatctg caaccgggaa
    22381 cggtctgtgg atgaccctgt ggctggcaat tccggtaatg gtgttctcgt tcaaccactc
    22441 tccgatcatc tcttctttcg ccgttgcgaa gcgtgaagag tacggcgata tggcagaaca
    22501 gaaatgctcg aagatccttg cattcgcaca catcatgatg gtgctgaccg taatgttctt
    22561 cgtcttcagc tgcgtactga gcctgactcc ggcagacctg gctgcggcta aagagcagaa
    22621 catctcgatt ctgtcttacc tggctaacca ctttaacgca ccggttatcg cgtggatggc
    22681 tccgattatc gcgattatcg ctatcaccaa atccttcctc ggccactacc tgggcgcacg
    22741 tgaaggcttc aacggtatgg tgattaaatc tctgcgtggt aaaggtaagt ctatcgaaat
    22801 caacaagctg aaccgtatca ctgcgctgtt catgctggta acgacctgga ttgttgccac
    22861 cctgaacccg agcatcctgg gtatgattga aaccctgggc ggcccaatca tcgcgatgat
    22921 cctgttcctg atgccgatgt acgcaattca gaaagtaccg gcaatgcgta agtacagcgg
    22981 tcacatcagc aacgtattcg ttgtcgtgat gggtctgatt gcaatctccg caatcttcta
    23041 ctctctgttc agctaagtcc tttcgcgccg ctttcgggcg gcgcttcctc cgttttaacg
    23101 cgatgtattt cctatgatta gcgtattcga tattttcaaa atcggcattg gcccttccag
    23161 ttctcatacc gttggaccaa tgaaagcggg taaacaattt accgacgatc tgattgcccg
    23221 taatctgctt aaagacgtga cccgcgtggt ggttgacgtg tacggctcgc tctctctgac
    23281 cggtaaaggc caccacactg atatcgccat tattatgggc ctggcgggta acctgccgga
    23341 taccgtggat atcgattcca tccctggttt tattcaggat gtgaatactc atggtcgcct
    23401 gatgctggca aacggtcagc atgaagtgga gttcccggtt gatcagtgca tgaacttcca
    23461 tgcagacaac ctttctctgc atgaaaacgg tatgcgcatt accgcgctgg cgggcgataa
    23521 agtcgtttac agccagactt actactctat tggcggtggc tttatcgttg atgaagagca
    23581 ttttggccag cagaatagcg caccggttga agttccttat ccgtacagtt cagcagccga
    23641 tctgcaaaaa cattgtcagg aaaccgggct gtcactctct ggcctgatga tgaaaaatga
    23701 gctggcgctg cacagcaaag aagagctgga acagcacctg gcgaacgtat gggaagtcat
    23761 gcgtggcggt attgagcgcg gtatttccac cgaaggcgtg ttgcctggca aactgcgcgt
    23821 tccacgccgt gctgcggcac tacgccggat gctggtcagc caggataaaa ccaccactga
    23881 cccgatggcg gttgttgact ggatcaacat gtttgcactg gcagtgaacg aagagaacgc
    23941 tgctggcgga cgcgtggtga ctgcgccgac taacggtgcg tgcgggatta tcccggcagt
    24001 gctggcgtac tacgacaagt ttatccgcga agtgaacgct aactcactgg ctcgttacct
    24061 gctggtagcc agtgccattg gttctcttta taagatgaac gcgtcaattt ctggtgctga
    24121 agtcggctgc cagggtgaag ttggtgtggc gtgctcaatg gcggcggctg gtctggcaga
    24181 actgttaggt gcaagcccgg cgcaggtgtg catcgcggcg gaaatcgcca tggagcacaa
    24241 cctcggtctg acgtgtgacc cggtcgccgg acaggtccag gtgccatgca tcgagcgtaa
    24301 cgccattgcg gcagtaaaag cggtgaacgc cgcacgtatg gcgctacgcc gtaccagcga
    24361 gccgcgcgtc tgcctcgata aagttatcga aaccatgtac gaaacaggta aagatatgaa
    24421 cgccaagtac cgcgaaacct ctcgcggcgg cctggcaatg aagatcgttg cctgcgatta
    24481 atcgctctcc agaggcctcg ttttgcgagg cctcttcccg atttctcatc cagccgtagc
    24541 ctgttccggc atcgaatgtt acccttatcg cctgatcttt aagggggtta tcgtggctgt
    24601 tcatttgctt attgtcgatg cactgaatct tattcgtcgc attcatgccg ttcaggggtc
    24661 gccctgtgtc gaaacctgcc agcatgcgct cgatcagctc attatgcaca gccagccaac
    24721 ccacgcggtc gccgtttttg atgatgaaaa ccgcagtagc ggctggcgtc atcagcgttt
    24781 accagattac aaagcgggtc gaccgccaat gccggaagaa ttgcacgacg agatgcctgc
    24841 attacgcgcc gcctttgagc aacgcggcgt cccgtgctgg tcaaccagcg gcaacgaagc
    24901 cgatgactta gccgccacgc tggcggtcaa agtgacacag gccgggcatc aggcaacgat
    24961 tgtttcgaca gataaaggct actgtcagtt actttcaccg acattacgta ttcgtgatta
    25021 cttccagaaa cgttggctgg atgcgccatt tatcgataaa gaatttggcg ttcaaccgca
    25081 gcagttgccc gattactggg gacttgcggg gatcagcagt tcaaaggtac cgggtgttgc
    25141 gggaatcgga ccaaaaagcg ccacgcagct gctggtcgag tttcagagtc tggaagggat
    25201 atatgagaat ctggatgcgg ttgccgaaaa gtggcgcaaa aaattagaaa cccataaaga
    25261 gatggcgttt ctgtgccgcg atattgcccg cttacaaacc gatttgcata tcgacggcaa
    25321 tttacagcaa ttgcggttgg tacggtaacg gcgagccgga tacgccgcaa acgtcgtatc
    25381 cggcattatc acatcagcgc atttaccagg cggtatggta aagctctaca atatcctcaa
    25441 gcgttgcttc acgcgggttg ccaccggtac aaacatcatc cagtgccgcc tgcgccagtg
    25501 ccggaatgtc ttccttgcgt acaccaacat cacgcaaatg tggcggaata ccgacatcac
    25561 ggttgagagc aaacaccgct tcaacagcgg cattacgcgc ctcttccagg ctcatacctt
    25621 ccactttcac gcccataacg cgcgcgatat cgcggtactt ctcaccggta aagtcagcgt
    25681 tataacgcat gacatgcggt aacaggatgg cgttcgcaac accgtgtgga gtgttataaa
    25741 acgcgcccag tggatgcgcc ataccatgca ccacccctaa cccaacattc gagaagccca
    25801 tacccgcaac atactgcccg agcgccattt cttctccggc atccttatca ccagcaaccg
    25861 atcctcgcag cgccccagca atgatttcaa tcgctttaat gtgcagtgca tcggttagcg
    25921 cccacgcgcc acgggtaata tacccctcaa tagcatgagt gagcgcatcg acacccgtcg
    25981 cagctttcag cgctggaggc ataccatcca tcatgtcagc gtcaataaac gccacctgcg
    26041 ggatatcatg cggatcaacg caaacaaact tgcgccgttt ctcttcgtca gtgatcacgt
    26101 agttaatggt cacttctgcc gcagtacctg ctgtggtagg aattgccaga atcggtacac
    26161 tgggtttatt ggtcggggaa agcccttcca ggctacgcac atcggcaaac tccgggttgt
    26221 tgctgataat gccaatcgct ttacaagtat cctgtggaga accaccacca atagcgatca
    26281 ggtaatccgc gccgctattc tggaatacac cgagcccttc tttgacgaca gtaattgttg
    26341 ggttgggcac tacgccgtcg taaatcgccc atgccagccc tgcagcatcc atcttatcgg
    26401 tcactttcgc caccacgccg cattgcacca gcgttttatc ggtgacgatc agcgccttct
    26461 gataaccacg gcgtttcacc tcatcggtta aagccccaac agtaccccga ccaaaccatg
    26521 ccgtttcgtt cagaatcatt ctgttagcca tcatccttct ccttgttgct ttacgaaatt
    26581 actcttcaat tcgtaaccca taggttttga atttctccag cactacggca atctcttcat
    26641 cgctcagcac tggcaccggg tccgtaatcg ccagggtcgt caggtaaagt tgcgccagca
    26701 cttcaacttc atgcgccagc cataacgctt tttccagatt cacctcacaa gcgataagcc
    26761 catgatgttg taacaaagtt gccttacgat ttttgagagc cagcgcaaca tgttcagaaa
    26821 gttcgcgtgt tccaaaggtc gcataaggcg cgcaaggaat agaattaccg ccagccgccg
    26881 caatcatgta gtgaatagcg gggatcgatc ggttaagaat ggaaactgcc gtgcaatgaa
    26941 cggcatgatt gtgaacaacc gcgttggcat ccggtctgct ttgataggct gccatatgga
    27001 aacgccattc gcttgagggg agctttcctt cctcatgttt accgttgcca tcaataaaga
    27061 caatatgcga ctccgtcagt ttttcatatg gaatgcctgt aggcgtaatc agcatcccat
    27121 cctgataacg tacactgacg ttccccgctg tcccctggtt cagtcccagg cgggtcattt
    27181 ccaggcaagt gtcaataatc tgacgagcaa gtttatttcg ttccattagc tacctctctc
    27241 tgattcaaaa cagggcaata atgttgttcc tttcacacta ttgaattagc cgtttaatta
    27301 cccaccatct tcttcctgat taacaagaaa gaaattcaca agcttatatt ttgtgacctg
    27361 gttcaactaa tcacagtaaa taactgcaag ttctcttttt ataaccccat taaaaatgac
    27421 cgctcttaaa aatatttatc aaaacggtca tttttctatt cctccaagcc cggaatgacc
    27481 gttttcggca caaacaatta atacggtcat ctgatttgtg ttttttatga tttattttct
    27541 gaaacgggca tgaaatttcg attattaaag tgatggtagt cacataaagt caccttctag
    27601 ctaataagtg tgaccgccgt catattacag agcgtttttt atttgaaaat gaatccatga
    27661 gttcatttca gacaggcaaa tattcactga tatgaagccc gaactcgctg gttttgcact
    27721 tttgaaaaca taaccgatta cgtgcttaag cttctgaacc taagaggatg ctatgggaaa
    27781 cacatcaata caaacgcaga gttaccgtgc ggtagataaa gatgcagggc aaagcagaag
    27841 ttacattatt ccattcgcgc tgctgtgctc actgtttttt ctttgggcgg tagccaataa
    27901 ccttaacgac attttattac ctcaattcca gcaggctttt acgctgacaa atttccaggc
    27961 tggcctgatc caatcggcct tttactttgg ttatttcatt atcccaatcc ctgctgggat
    28021 attgatgaaa aaactcagtt ataaagcagg gattattacc gggttatttt tatatgcctt
    28081 gggtgctgca ttattctggc ccgccgcaga aataatgaac tacaccttat ttttagttgg
    28141 cctatttatt attgcagccg gattaggttg tctggaaact gccgcaaacc cttttgttac
    28201 ggtattaggg ccggaaagta gtggtcactt ccgcttaaat cttgcgcaaa catttaactc
    28261 gtttggcgca attatcgcgg ttgtctttgg gcaaagtctt attttgtcta acgtgccaca
    28321 tcaatcgcaa gacgttctcg ataaaatgtc tccagagcaa ttgagtgcgt ataaacacag
    28381 cctggtatta tcggtacaga caccttatat gatcatcgtg gctatcgtgt tactggtcgc
    28441 cctgctgatc atgctgacga aattcccggc attgcagagt gataatcaca gtgacgccaa
    28501 acaaggatcg ttctccgcat cgctttctcg cctggcgcgt attcgccact ggcgctgggc
    28561 ggtattagcg caattctgct atgtcggcgc acaaacggcc tgctggagct atttgattcg
    28621 ctacgctgta gaagaaattc caggtatgac tgcaggcttt gccgctaact atttaaccgg
    28681 aaccatggtg tgcttcttta ttggtcgttt caccggtacc tggctcatca gtcgcttcgc
    28741 accacacaaa gtcctggccg cctacgcatt aatcgctatg gcactgtgcc tgatctcagc
    28801 cttcgctggc ggtcatgtgg gcttaatagc cctgacttta tgcagcgcct ttatgtcgat
    28861 tcagtaccca acaatcttct cgctgggcat taagaatctc ggccaggaca ccaaatatgg
    28921 ttcgtccttc atcgttatga ccattattgg cggcggtatt gtcactccgg tcatgggttt
    28981 tgtcagtgac gcggcgggca acatccccac tgctgaactg atccccgcac tctgcttcgc
    29041 ggtcatcttt atctttgccc gtttccgttc tcaaacggca actaactgaa catattttcc
    29101 gaataaagtg aggaatctgt aatgaaaaaa atcagcttac cgaaaattgg tatccgcccg
    29161 gttattgacg gtcgtcgcat gggtgttcgt gagtcgcttg aagaacaaac aatgaatatg
    29221 gcgaaagcta cggccgcact gctgaccgag aaactgcgcc atgcctgcgg agctgccgtc
    29281 gagtgtgtca tttccgatac ctgtatcgcg ggtatggctg aagccgctgc ttgcgaagaa
    29341 aaattcagca gtcagaatgt aggcctcacc attacggtaa cgccttgctg gtgctatggc
    29401 agtgaaacca tcgacatgga tccaacccgc ccgaaagcca tttggggctt taacggcact
    29461 gaacgtcccg gcgctgttta cctggcagcg gctctggcag ctcacagcca gaaaggcatc
    29521 ccggcattct ccatttacgg tcatgacgtt caggatgccg atgacacatc gattcctgcc
    29581 gatgttgaag aaaaactgct gcgctttgcc cgcgccggtt tggccgtcgc cagcatgaaa
    29641 ggtaaaagct atctgtcgct gggcggcgtt tcgatgggta tcgccggttc tattgttgat
    29701 cacaacttct ttgaatcctg gctgggaatg aaagtccagg cggtggatat gaccgaactg
    29761 cgtcgccgta tcgatcagca gatttacgac gaagccgaac tggaaatggc gctggcctgg
    29821 gctgataaaa acttccgcta tggcgaagat gaaaataaca aacagtatca acgtaatgcc
    29881 gagcaaagcc gcgcagttct gcgcgaaagt ttactgatgg cgatgtgtat ccgcgacatg
    29941 atgcaaggca acagcaaact ggccgatatt ggtcgcgtgg aagaatcact tggctacaac
    30001 gccatcgctg cgggcttcca ggggcaacgt cactggaccg atcaatatcc caatggtgac
    30061 accgccgaag cgatcctcaa cagttcattt gactggaatg gcgtgcgcga accctttgtc
    30121 gtggcgaccg aaaacgacag tcttaacggc gtggcaatgc taatgggtca ccagctcacc
    30181 ggcaccgctc aggtatttgc cgatgtgcgt acctactggt caccagaagc aattgagcgt
    30241 gtaacggggc ataaactgga tggactggca gaacacggca tcatccattt gatcaactcc
    30301 ggttctgctg cgctggacgg ttcctgtaaa caacgcgaca gcgaaggtaa cccgacgatg
    30361 aagccacact gggaaatctc tcagcaagag gctgacgctt gcctcgccgc taccgaatgg
    30421 tgcccggcga tccacgaata cttccgtggc ggcggttact cttcccgctt ccttaccgaa
    30481 ggcggcgtcc cgttcaccat gactcgtgtc aacatcatca aaggcctggg accggtactg
    30541 caaatcgcgg aaggctggag cgtggaattg ccgaaggatg tgcatgacat cctcaacaaa
    30601 cgcaccaact caacctggcc aaccacctgg tttgcaccgc gcctcaccgg taaagggccg
    30661 tttacggatg tgtactcggt aatggcgaac tggggcgcta accatggggt tctgaccatc
    30721 ggccacgttg gcgcagactt tatcactctc gcctccatgc tgcgtatccc ggtatgtatg
    30781 cacaacgttg aagagaccaa agtgtatcgt ccttctgcct gggctgcgca cggcatggat
    30841 attgaaggcc aggattaccg cgcttgccag aactacggtc cgttgtacaa gcgttaatac
    30901 attactccct gatgtgatgc ccggtcgctc cggctaccgg gcctgaacaa gcaagagtgg
    30961 ttagccggat aagcaatgtt atccggctat attgcaggag cgattatgaa acaagaagtt
    31021 atcctggtac tcgactgtgg cgcgaccaat gtcagggcca tcgcggttaa tcggcagggc
    31081 aaaattgttg cccgcgcctc aacgcctaat gccagcgata tcgcgatgga aaacaacacc
    31141 tggcaccagt ggtctttaga cgccattttg caacgctttg ctgattgctg tcggcaaatc
    31201 aatagtgaac tgactgaatg ccacatccgc ggtatcgccg tcaccacctt tggtgtggat
    31261 ggcgctctgg tagataagca aggcaatctg ctctatccga tcattagctg gaaatgtccg
    31321 cgaacagcag cggttatgga caatattaaa cggttaatct ccgcacagcg gttgcaggct
    31381 atttctggcg tcggagcctt tagtttcaat acgttatata agttggtgtg gttgaaagaa
    31441 aatcatccac aactgctgga acgcgcgcac gcctggctct tcatttcgtc gctgattaac
    31501 caccgtttaa ccggcgaatt cactactgat atcacgatgg ccggaaccag ccagatgctg
    31561 gatatccagc aacgcgattt cagtccgcaa attttacaag ccaccggtat tccatgccga
    31621 ctcttccctc gtctggtgga agcgggtgaa cagattggta cgctacagaa cagcgccgca
    31681 gcaatgctcg gcttacccgt tggcataccg gtgatttccg caggtcacga tacccagttc
    31741 gccctttttg gcgctggtgc cgaacaaaat gaaccagtgc tctcctccgg tacatgggaa
    31801 attttaatgg tccgtagcgc ccagattgat acttcgctgt taagtcagta cgccggttcc
    31861 acctgcgaac tggatagcca ggcagggttg tataacccag gtatgcaatg gctggcatcc
    31921 ggcgtgctgg aatgggtgag aaaactgttc tggacggctg aaacaccctg gcaaatgttg
    31981 attgaagaag ctcgtctgat cgcacctggc gcggatggcg tgaaaatgca gtgtgattta
    32041 ttgtcgtgtc agaacgctgg ctggcaagga gtgacgctta atactacgcg ggggcatttc
    32101 tatcgcgcgg cgctggaagg gttaaccgcg caattacagc gcaatctaca gatgctggaa
    32161 aaaatcgggc actttaaagc ctctgaatta ttgttagtcg gcggaggaag tcgcaacaca
    32221 ttgtggaatc agattaaagc caatatgctt gatattccgg taaaagttct cgacgatgcc
    32281 gaaacgaccg tcgcaggagc tgcgctgttc ggttggtatg gcgtagggga atttaacagc
    32341 ccggaagaag cccgtgcaca gattcattat cagtaccgtt atttctaccc gcaaactgaa
    32401 cctgaattta tagaggaagt gtgaaatgct gaaaacaatt tcgccgttaa tttctcccga
    32461 actattgaaa gtgctggcag agatgggaca tggagatgaa attatttttt ccgatgctca
    32521 ctttcccgcc cattcgatgg gaccgcaggt gatccgcgct gatggcctgt tggtgagcga
    32581 cttgctccag gcgattatcc cgttatttga actggacagt tatgcaccgc cgctggtgat
    32641 gatggcagcg gtagaaggtg atactctcga tcctgaagta gaacgacgtt accgtaatgc
    32701 gctttcacta caagccccgt gtcctgacat catccgcatc aatcgttttg cgttttatga
    32761 acgggcgcaa aaagcctttg cgatcgttat cacaggcgaa cgagcgaagt acgggaatat
    32821 tcttttaaaa aaaggggtaa caccgtaatc tcataccggt acgcccgcat gacgcgggcg
    32881 gttatcgaat gatggggtga aaaatatgaa agcggcacgc cagcaagcga tagtcgacct
    32941 gctgctgaac cataccagcc tgaccacgga agctctctct gaacagctaa aggtcagtaa
    33001 agaaaccatt cgtcgcgatc tcaatgaatt acagacgcag ggtaaaattc tgcgcaatca
    33061 tggacgcgct aaatatatcc accgtcaaaa tcaagacagt ggcgatccct ttcacatccg
    33121 gctgaaaagc cattatgcgc ataaagcaga tatcgcgcgc gaggcgctcg cgtggattga
    33181 agaagggatg gtgatagcct tagacgccag ttcaacttgc tggtatctgg cacgccagtt
    33241 gcctgacatc aacattcagg tcttcaccaa tagccatccg atttgccatg aactcggtaa
    33301 acgcgaacgc attcaactga tcagttccgg cggcacactt gagcgcaaat atggctgtta
    33361 cgtcaatccc tcgctgattt cccaacttaa atcgctggaa atcgatctgt ttattttttc
    33421 ttgtgaaggg atcgatagca gcggcgcact gtgggactcc aatgccatca acgctgatta
    33481 caaatcgatg ctattaaaac gtgccgcgca atcgttgtta ttgattgata aaagtaaatt
    33541 taatcgttca ggggaagccc gcatcgggca tctggatgag gtaacgcaca ttatttctga
    33601 tgagcgccag gttgcaactt ctttggtaac agcctgacgg aaagggtagc aggccggaga
    33661 cgacgccccg gccttgcctg ttatcgctcg tcgcgacgac cacccaccgc agcccagatg
    33721 cggcggacgt gcaccgtcac ttcttcgcga tcgtgataca actgccgcgc ctgaatctga
    33781 gcatttatgc catgttcatc aagctgtgcc tgaatatacg ccagattgtg tgacacttct
    33841 tcgtagcgtt ttttcatcgg cagtttgagg ttgaaaatgg tttcacgaca ccagccatta
    33901 accagccact gcgccatcaa tgccgcaact ttcgccggtt tttcaaccat atcgcatacc
    33961 atccaggaga tattgctgcg cgtcggacgg aatttgaaac cgtcttcccg cagccacgtc
    34021 acctgtccgg tatccatcag actttgcgcc atcgggccgt tgtcgacgga ataaacccac
    34081 atgttgcgct tcaccagttg gtaggtccag ccgccagggc aagcgcctaa atccaccgcc
    34141 cacatcccgt tcgccaggcg ttcatcccac tcatccgcag gaataaacac atgaaatgcc
    34201 tcttccagtt tgagcgtgga acgactcggc gcatctgccg gaaatttcag gcgcggaatg
    34261 cccatataga acggcgaatt attgttgctg tatgagtaac cggtatagca gcagcctggt
    34321 gcaataaaga atacatgcac aaccggacgc ttcggcgttt catagttcgc cagcaccccg
    34381 gcatcgcgca aggcagcgcg tagcggaaca gtaaatttac ggcagaattt cagcaactct
    34441 ttgctttcgt tggtatcggc aacttcaaca cgcagttcac cgcccttctc tactacgccc
    34501 tgtaacatgc cgacaatggg ggtaatacga tcttctggcg gcaagtgctg caggagttcc
    34561 cccaccacaa accactggcg ggcaaaaatt aatgaactga acggcagctc acggattaac
    34621 ttatcgccat catcaggttg ataacattca taaatgacat aacccgcatt ctctttcacg
    34681 cgggcaaaac cgaaaatttc ccgctggccg gctttatcgg taatttctgc ggcgcactct
    34741 ttttcaaagc ccggacggca cagcaataca accttattca tgacttacgc ccttgcgctt
    34801 taaacggata gcaccaacta acattaacgc ccagcccgcg aggaagctca cgccgccaac
    34861 cggagtgaca aacgcccaca aacgcagatg ggacagcgcc aggcaataaa ggctgccgct
    34921 gaacaacacc gtgcctaacg cgaggaaaac gctactccag taaaaccaga tactgatgcg
    34981 acgctgcatt gccaccgcca gacctaagat cgccagcgta tgaaacgcct ggtattcgag
    35041 gccggtctgg atccagccca tctcaacggc ccccatggtt ttacttaaca catgcgcgcc
    35101 aaaagcgccc agagccacaa aaatgaagcc gctaatggcg gcgaaaatca gcataaaacg
    35161 gctggtcatg gtcgtaccct acgtaaatta ttgttcataa cgaaagcgga atttttcttg
    35221 ttcagcagcg gctttcgcca ggatccattg gcgaaaggcg gctattttac ccagttctgc
    35281 ctggctgtca tgacaaacca gataaaaagc gtttttactg accagaacat cattaaacgg
    35341 gcaaacaaga cgtccggcct cgatttcaga ttgcgccatc acgttatttg ccagcgccac
    35401 tccctgcccg tgaatagccg cttgcagcac catggcgcta tgactaaaaa ttggcccttg
    35461 ctgaacgttg atatgattta accccaactg tcgggtatat gtctgccagt cacggcgtga
    35521 cgcatcatgt aataacgtat gtttagccag atcttccggg gtcttcaagg gtttttcgcc
    35581 tgtcagcagt agcggcgaac acaccggcaa taaatattcg gcgtacagtt tttccacccg
    35641 tagccccggc cagttgcccc gaccataaaa tatcgccaca tcaacatcat ccgccagctt
    35701 atcttcctga cggtcaaccg cctggattcg aacgtcaatt cccggataag ctgaattaaa
    35761 gctggaaagt cgcggaacca accaatgaat ggcgaaactg gggagtaaac tgaccgtcaa
    35821 cgcccccttg gcgctacggg cctggagttt acgcgtcgct tcggttaatt gcgaaaatat
    35881 ctctttgata tcgaggaaat agctttgacc ttcctcggtc agcaggagtg aacgattacg
    35941 gcggcggaac agttttagcc ccagaaaatc ctcaagagac ttgatttgat gacttactgc
    36001 ggcctgggtc acaaaaagct cttctgctgc gcgagtgaaa cttaaatggc gtgctgcggc
    36061 atcaaaaact cgtaaggcat ttagcggtgg taatcgttta gacatggctt ttaaactttg
    36121 atgttaaatg aatttaacaa ttagatcaca ctatgtaacc tattagtttt tttaatctga
    36181 gccattataa attgtccgtt gagcttctac cagcaaatac ctatagtggc ggcacttcct
    36241 gagccggaac gaaaagtttt atcggaatgc gtgttctggt gaacttttgg cttacggttg
    36301 tgatgttgtg ttgttgtgtt tgcaattggt ctgcgattca gaccatggta gcaaagctac
    36361 cttttttcac ttcctgtaca tttaccctgt ctgtccatag tgattaatgt agcaccgcct
    36421 aattgcggtg ctttttttta ccttgcgatc gtgaattact gatccagttc gaccatctct
    36481 ttcacgtcag tacggttgat ctgctgtttg ttgccattag cgtctttata cgaaatcata
    36541 ccggtatcgt tatcagtctg tggtttgccg tcagagacga tggtacgtcc gtcattggtg
    36601 tgcatcacat agttcgaacc ggaacaggcg ctcagggcaa aagtcagcat acaggcagaa
    36661 ataattgcgg cagtcttttt catcatcttc tccttaaagc gattaataaa tcttaatagt
    36721 attgattaac aggctaaaat taacgcctaa cactattcag catatgttac ttacgcggct
    36781 tcgccaggat atccagataa ttctgatggt tagcactctc cttgtatcaa agtgaatttt
    36841 gcgtcacgat cggtgcatca agccgaggag taccatgaac gtttttaatc ccgcgcagtt
    36901 tcgcgcccag tttcccgcac tacaggatgc gggcgtctat ctcgacagcg ccgcgaccgc
    36961 gcttaaacct gaagccgtgg ttgaagccac ccaacagttt tacagtctga gcgccggaaa
    37021 cgtccatcgc agccagtttg ccgaagccca acgcctgacc gcgcgttatg aagctgcacg
    37081 agagaaagtg gcgcaattac tgaatgcacc ggatgataaa actatcgtct ggacgcgcgg
    37141 caccactgaa tccatcaaca tggtggcaca atgctatgcg cgtccgcgtc tgcaaccggg
    37201 cgatgagatt attgtcagcg tggcagaaca ccacgccaac ctcgtcccct ggctgatggt
    37261 cgcccaacaa actggagcca aagtggtgaa attgccgctt aatgcgcagc gactgccgga
    37321 tgtcgatttg ttgccagaac tgattactcc ccgtagtcgg attctggcgt tgggtcagat
    37381 gtcgaacgtt actggcggtt gcccggatct ggcgcgagcg attacctttg ctcattcagc
    37441 cgggatggtg gtgatggttg atggtgctca gggggcagtg catttccccg cggatgttca
    37501 gcaactggat attgatttct atgctttttc aggtcacaaa ctgtatggcc cgacaggtat
    37561 cggcgtgctg tatggtaaat cagaactgct ggaggcgatg tcgccctggc tgggcggcgg
    37621 caaaatggtt cacgaagtga gttttgacgg cttcacgact caatctgcgc cgtggaaact
    37681 ggaagctgga acgccaaatg tcgctggtgt cataggatta agcgcggcgc tggaatggct
    37741 ggcagattac gatatcaacc aggccgaaag ctggagccgt agcttagcaa cgctggcgga
    37801 agatgcgctg gcgaaacgtc ccggctttcg ttcattccgc tgccaggatt ccagcctgct
    37861 ggcctttgat tttgctggcg ttcatcatag cgatatggtg acgctgctgg cggagtacgg
    37921 tattgccctg cgggccgggc agcattgcgc tcagccgcta ctggcagaat taggcgtaac
    37981 cggcacactg cgcgcctctt ttgcgccata taatacaaag agtgatgtgg atgcgctggt
    38041 gaatgccgtt gaccgcgcgc tggaattatt ggtggattaa tgacaaaccc gcaattcgcc
    38101 ggacatccgt tcggcacaac cgtaaccgca gaaacgttac gcaatacctt cgcaccgttg
    38161 acgcaatggg aagataaata tcgccagttg atcatgctgg ggaaacagct tccggcattg
    38221 ccagacgagt taaaagcgca ggctaaagag attgccggat gcgaaaaccg cgtctggctg
    38281 ggatatacag tggctgaaaa cggcaaaatg catttctttg gcgacagcga agggcgcatt
    38341 gtgcgcggcc tgctggcggt gttgttgact gccgttgagg ggaaaaccgc cgccgagttg
    38401 caggcacagt caccactggc attgtttgat gagctgggat tacgtgcgca gcttagcgcc
    38461 tcacgcagcc aggggttaaa tgcgttaagc gaggcgatta tcgctgcgac gaagcaggtt
    38521 taagcacaga accgggtcgg ataagacgtt cgcgtctcat ccgacctgat tgtttaaccc
    38581 tgacgcgccg ctttcgccat catcttcttc agcgcatgag aaaccgcaac aaacccaaag
    38641 gtggcggtca ccatcgttgc cgcgccaaat ccagatgcac aatccatccg cttcggccct
    38701 tctgccgtgg ctttcatcgc acacaccgta ccgtctgact gcgggtacac cagcgcttca
    38761 gtagaaaaca cgcaatccac gccgagctta cctttactgt ttttcactac gccaaaatcg
    38821 cttttcaggc gctcgcgcaa cttcgccgcc agcgggtcct gaatcgtttt cgccagatcg
    38881 gtaacctgaa tctgcgtcgg ttcaatctgc ccacccgcgc caccggttgt gaccagtggg
    38941 attttattgc gccgacaata agcaatcagc gccgctttgg gccgtacact atcaatggca
    39001 tcaatcacgt acgaataacc cacgctcata tactgcgcta cgttatccgg cgtcacgaaa
    39061 tcatccacca ccgttacacg gcactccggg ttaatctggc gaatacgctc cgccataact
    39121 tccgcttttg ccagcccaac gttatcgcgc agggcatgaa tttgccgatt ggtattggtg
    39181 acgcacacat catccatatc gataagcgtg attgcgccaa tccccgtgcg cgccagcgct
    39241 tccgccgccc aggaaccgac accaccgata ccaaccacac aaatatgcgc gtcagcaaac
    39301 agttgcaacg ctttttcacc atacagacgc gctgtgccac caaaacgctg acgccatgca
    39361 tcactaatta ccacagacat agcacctcag aattaaaaag ggcgaggtta tccctcaccc
    39421 taaacgcata aatgcctgat gcgctacgct tatcaggcct acataactcc tgcaatattt
    39481 tggatttgca tgatttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg
    39541 cgcacttagt caataatcag gatgtgaggt tacccctcac cctgtcatat ccgtaaaaac
    39601 ggcatacaga atatcacatc agccgctaaa gacgttacct gcgcccgggg cggttttcag
    39661 cacccagaca cgtccatagt ggttgtacca acctgcgcgg tgtccggctt ccggcccgat
    39721 cccttgatag atatcgaagt gttggccttt gattgcacca ccgacatcca gcgccaccat
    39781 cagacgcagt tcgtactgac cattaaattt gccgttatta tccagcaacg gcacttctgc
    39841 cagcaaggta gtacctggcg gaataatgga acgatcagag gcaactgacg cgcgaccaac
    39901 cagcggcacc gcacttgccc ctttcaccgg tgcaaaagat tgcggtttaa agaaaacgaa
    39961 agacgggttc tgttccagca gctcgcgaac ctccgcttca ctgtgtgttt cgccccagtg
    40021 acgaatcgcc tgcatcgaca tatcttcttt tttcacttcg ccacggtcga tcaacacctt
    40081 accgatgctg cgataggcat gaccgttttt ccctgcatag ctgaaaaagt taagcggact
    40141 gccatcacca aagtcgatat acccactacc ctgcacatcc ataatgaagt tatccatcag
    40201 ggagttactg taagcgagaa tatatttatc actcaatgcc cccgcgtaga tctccgcacg
    40261 agacggcaga cgaccacgtt ttggcggcat acggtaaata ggatactgga actcgccctg
    40321 gcgggtatgg cgcgcctgaa ttaccggcgt gtaatagcct gtaaactgca cgttaccata
    40381 gttgtcggca ccttccatct gccaggcatc aatgccgaac tggcgcatat tgcgggtatc
    40441 accgcctgcg cgcagccact cttgcaccgc gttataaaca ttactctggt tgccatacag
    40501 acgcggtgac gaattacgga tatggttaat ttgctcggca aaatcaccgg cgttaatcgg
    40561 cgcgccaacg gcatctggct ggttcaccag agagaaaggc tgggtaaatt tcccgtcttt
    40621 atattgctgt ccgcgatcgg ttggtttgga agagcaggcg gcaagcattg ccacaaccgt
    40681 gcccataaga aggtacttta cccaacgtcc tttcattgtt ctcttcttca ggttaaaaaa
    40741 taaggcgcaa cgaagataac aaaccgccgc gtttaatgaa atgagcagcc tctccctgac
    40801 gcaaattttg cacaaaaaat aggctttagt gatttgtttt tgttcaaaat catgccaaat
    40861 ccgtgatcgg ggtaaaaaaa aggttgcatg aaaacgcgag cggagtatag tgcgcatcca
    40921 cggacgcggg gtggagcagc ctggtagctc gtcgggctca taacccgaag gtcgtcggtt
    40981 caaatccggc ccccgcaacc aattattgaa caccctaacg ggtgtttttt tgtttctggt
    41041 ctcccataaa aaagcgccat tcagcgcctt tttatcatcc ccttctcgcc agcgtcgccc
    41101 catcggcaaa atacgcttta attcccgcaa gaatagactc cgcaacttcc tgctggaaag
    41161 tcgccgtttt cagtttacgc tcttcctcaa cgttactgat aaacgccgtt tcgaccagaa
    41221 tggagggaat atctggtgcc tttagtacgg caaacccggc ctgttcaacc tgatttttat
    41281 gcagcttgtt gattttaccg agcttattca gtaccgcttt accaaactta aggctgtcag
    41341 caatggtcag cgactgcacc atatcgaaca tggtgtggtc gacatagcgg tcaccgcttt
    41401 tgctcacgcc accaatcaag tccgaggcgt tctgggtttg tgccagatat tttgccgcag
    41461 tactggttgc acctttggtt gagagcgcaa acacagagga accgctcggc tgacgactgg
    41521 taaaggcgtc ggcatggata gagacaaaca ggtcagcacg ctgtttctgg gcttttgcta
    41581 cgcgcacttg caacggaatg aagatgtctt cattgcgcgt catgtacacc ttcatattgc
    41641 cctctttctc gatcagagag cgcagacggc gagctatttg caataccacg tctttttcgc
    41701 gcgttttgta tttccccacc gcaccggagt cttcgccacc gtggccaggg tcaagcataa
    41761 tgacaatcgg acgatcgcgc cctgctttac ctggttgtgg accactttgc gctggcggca
    41821 cctgcttttc gaggtcgcct ttgttgtaat cctccagcag cgccagcagc gggtcctgca
    41881 tatcctgtgc attggccgga tagaggtcca tcaccagacg ctctttaaac ccggcgaccg
    41941 gcgcaagggc aaacagctgc ggttttacgt tttgctttaa ttcaaaaacc atccgtacgg
    42001 tttgcgggtc aaattgcccg acgcgcgccg acttgatgaa cgggtcgtct gcacgaattt
    42061 gtgccgccat ccccttgagt accgagttca ggtttacatc ttcgatatcc accaccacgc
    42121 gttccgggtt actcaacgcg aactgcttat atttcagctg acgatttgat tctaccgtca
    42181 cgcgggtgta gctggacgca ggccagacgc gcaccgccac gacctggctg accgcagcca
    42241 gactgacctg acttacgctc aatagccaca tggcacccgc gccttgcagt aaacgacgac
    42301 ggctgattgc agtgttggat cctgacatgc ctctcccgag caaaagaaat ctaagctgtg
    42361 taacaagtaa acgactaatt tgaccggaaa ctttagcgaa agacgcataa tctgtcatct
    42421 aataaacggt aaacattctt tttatattca cggcattact gataaaaaag tcgctctcgc
    42481 ataaaattta cacttgcacc ctgcgaaaaa acagaataaa aatacaataa tttcgaataa
    42541 tcatgcaaag aggtgtgccg tggtaaagga acgtaaaacc gagctggtcg agggattccg
    42601 ccattcggtt ccctatatca atacccaccg gggaaaaacg tttgtcatca tgctcggcgg
    42661 tgaagccatt gagcatgaga atttctccag tatcgttaat gatatcgggt tgttgcacag
    42721 cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg cagatcgacg caaatctggc
    42781 tgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt gtgaccgacg ccaaaacact
    42841 ggaactggtg aagcaggctg cgggaacatt gcaactggat attactgctc gcctgtcgat
    42901 gagtctcaat aacacgccgc tgcagggcgc gcatatcaac gtcgtcagtg gcaattttat
    42961 tattgcccag ccgctgggcg tcgatgacgg cgtggattac tgccatagcg ggcgtatccg
    43021 gcggattgat gaagacgcga tccatcgtca actggacagc ggtgcaatag tgctaatggg
    43081 gccggtcgct gtttcagtca ctggcgagag ctttaacctg acctcggaag agattgccac
    43141 tcaactggcc atcaaactga aagctgaaaa gatgattggt ttttgctctt cccagggcgt
    43201 cactaatgac gacggtgata ttgtctcaga acttttccct aacgaagcgc aagcgcgggt
    43261 agaagcccag gaagagaaag gcgattacaa ctccggtacg gtgcgctttt tgcgtggcgc
    43321 agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta atcagttatc aggaagatgg
    43381 cgcgctgttg caagagttgt tctcacgcga cggtatcggt acgcagattg tgatggaaag
    43441 cgccgagcag attcgtcgcg caacaatcaa cgatatcggc ggtattctgg agttgattcg
    43501 cccgctggag cagcaaggta ttctggtgcg ccgttctcgc gagcagctgg agatggaaat
    43561 cgacaaattc accattattc agcgcgataa cacgactatt gcctgcgccg cgctctatcc
    43621 gttcccggaa gagaagattg gggaaatggc ctgtgtggca gttcacccgg attaccgcag
    43681 ttcatcacgg ggcgaggttc tgcttgaacg cattgccgct caggcgaagc agagcggctt
    43741 aagcaaattg tttgtgctga ccacgcgcag tattcactgg ttccaggaac gtggatttac
    43801 cccggtggat attgatttac tgcccgagag caaaaagcag ttgtacaact accagcgtaa
    43861 atccaaagtg ttgatggcgg atttagggta agcgacgaaa atcgccggat gcgacatgcg
    43921 taacactcgt gcgtcgcatc cggcaattac gtttattccc gcgaactaaa caacgccgcc
    43981 agaccactgc gccgctcagt acgagtggcg attgccgcac ttaatatacg ctcatcggca
    44041 tacagcgaca gacggcgacg cgcgcgggtc accgcggtgt ataccagctc tcgcgttact
    44101 accggcgtgc gttggctcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat
    44161 ttatgtaccg tcatcgccca cgtagtttcg tgctctggca agcgactcgg ttgcacagac
    44221 ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg atccagcgca
    44281 ataccgatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat catcaccggt
    44341 cggccttcgt accaacgaga gtgcggatga cgatgaattt tgcgcttctg ttgcataaac
    44401 tgctcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa
    44461 agctggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcagcaga
    44521 tccagataac gtccgtaacc cgcaagagct tcctcaagca tcgcaatata atcttcgccg
    44581 ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct gaaaaacggt tttcactgcc
    44641 gttttatcac cacgattgat cgccgcagct aactgaccaa tgccagaatc gctgccgaaa
    44701 cgatagcttt tttgcagcag gcagagactg tcgcgcaaag atgccgcttc tgtgccggtt
    44761 cctgccggaa cgtgagtccc cgtcaggcgg cttagctgcc tggcacgctc ggcggtaaag
    44821 cccgcgttgg cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt
    44881 tgatcacgat cgccgagaaa gatcactcgc gcatgatctg gcaaggcgtc gatcagtctc
    44941 gacatcatag gcagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc
    45001 gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcacccag caatcggtgc
    45061 aaagtgctgg catcttccgg aatgcgtttc ttttgttcat cgctcagcgg taactgtcgc
    45121 aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga
    45181 cggatacggc agcgttcgcc gtcggccatt tgaattaacg ctgccagcaa cttcgccacg
    45241 gtggtcgttt taccggtgcc tgggccgccg gaaatcaccg agatccgccg cgtcagcgcc
    45301 actgccgccg caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg
    45361 gtttgcgcca gtagagcttc atcaacctcg atggcatgat tcacctcatt gaagaagcgc
    45421 gctaccgtcc gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc acagaggatc
    45481 atcggcgtgg gttcatctcc ccggctgacc gcttgtgaag ccagcaagca ttcttcccaa
    45541 ttttgtagct caccgatttc actgacacag gtcgccaaca gcggatgcga cgcctcgtta
    45601 ttttccagtc gtgaaagcgg taaacagaca tgcccttccc cggcgtcgtg gcttaacagg
    45661 gctgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc aaattgcaca
    45721 tccagcgggc gtagctgttt gtgctccaca gcttccagta attgcttttg caatttcatt
    45781 acgcctcctc cagggtcata ccggcaaaca tctcatccat caggtcaatc aaccctgcat
    45841 tgggtcgggt tgtgtaaatc ccctgttgcg gatgttcttt atcaacgcca cgcaggaaca
    45901 gatagataac gccgccaaag tgacgatcat agtcgtaatc agcaatgcga tggcgcagat
    45961 aacgatgcag cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcatcg
    46021 ctgctgccat agcctgttgg gtgtaagccg aactgtcttc acccaaccag ttggatttat
    46081 agtcgagcag gtaataacgc ccttcgtggc ggaacaccag gtcgataaag ccttttaaca
    46141 tgccacgtac ctgcatgaac tccagcggcg ggcagcctgc ggatagcggg tcaaactggc
    46201 ggattaacgt atcaagctga ctggcgataa gcggttcact aatcggcaga taaaactcca
    46261 tctccacctg tttattgcgg gcggaaagtt gactcaggct tacgccggtt tcattgagag
    46321 gtgcctggag gacagccgtg atccactcgg tcaataccgg ttcccactgc gattcaaagc
    46381 cgccgagttc cagtttttcc cgcacccagt tcgggtcaac cggctgggta aaatccaggt
    46441 cttcaaacaa actgtgcaag aatgtccctg gtgacgcacc gcgcgggaac tgatgcggtg
    46501 ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca
    46561 tcagatcctg agcgataccg tgaccacgct gctgcaaacc ggagtagctg gtgacacgcc
    46621 agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt aaagcatcat
    46681 taacctgcca gggctgttta tcacccgttt gtgccgtttg ccaggcaata tcatcatcgc
    46741 ataacgcttc aatacaggtg cgaagccctg ccgcatcttg cggttcccct ttttgcagca
    46801 aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt tttatcgcca cgacggcgca
    46861 ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg tgtcagcgcc acgtaaagca
    46921 aacgcagatc ttccgccaga cgttcggcct ccgcgaggtc gacgctttct ggcgcagcat
    46981 taagatccag aactgcctca aacgagtggc gatcgtgata aaacgcctgc tcctggacgc
    47041 ggaaattggt gataaacggc agccagacca atggatattc cagccctttc gatttgtgga
    47101 tcgtgacaat ctgcaccaga tgtttatcac tttcgagacg catttgttgg ctggaggcat
    47161 tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca
    47221 gctgcgttcc ggcttcttgt agcagttcgc tgatatgcaa gatatcggta agacgccgct
    47281 caccgcctgc cgttgccagc aagttttctg caatgttacg cgccgacatc agcgcccgca
    47341 gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcaacta
    47401 ccgcatccca cgcatgttcg tcattattca gcgtttcgat atccagcgcg ttcagcccca
    47461 tcattgacgt tgccagcgca ctacgcaggg tgttctcacg ttcgggcgtc atcaccgcct
    47521 gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa aacactgtcg cggttcgaaa
    47581 ggtaaacgga agggatttcc agcaacgtta aggcatcgcg cacctgggcg gcctcctggc
    47641 ggctgcgcac cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca
    47701 tcagcaacgc ttcgcccctc tgtccggctt gtagccagtc gcggatttgc gcagcacata
    47761 cctgcgccat ggtactttga taatcgccaa cgccgcagct ttcgccttcc atcagccaca
    47821 ttttcatcgc aggctgcgtt tcacctttaa atacaaaacg taacgcctga tttttcccgg
    47881 ctgatttcac tggaataaac ggtatttcgc gaaacatgaa cgcgtcatca gtctggctga
    47941 aaagcttatt cacgctgttc accattcctg gtgcggaacg ccagttggtg tctaaagtgt
    48001 agtgggcgtg aacttcgcta cgcgccttca tataagtgaa gatatccgca ccccggaatg
    48061 catatatggc ctgcttcggg tcgccaatta gcaacaatgc ggtttccggc tgatggtgcc
    48121 agatacggcg aaaaattcgg tactgctgtg ggtcggtatc ctgaaattca tcgatcattg
    48181 ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc accgctttcg ctacgcagcg
    48241 cggaatcgag ccgacttaac atgtcatcaa aacccaattc gccacggcgg cgtttttcac
    48301 gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat caccagatcg cggatcgaca
    48361 atggttctgc aagcagttga tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc
    48421 cggccttcgt gcgatcttct aagaaacgct gggagaattt ttccagcgac tccggcaact
    48481 gataactgtt tgtctcttct tctgcccagg cgctgatctt gtcgatccat ttagcctgat
    48541 tgctacggtt aaacttgcgt cgctcaatac cagaagattc gatcagcgca tccagttcac
    48601 ccactgcgtc gcgccactgc tgttttaccg tatcaatacg cgccacaatt tgcgcgtgac
    48661 gggaagccag cgtttcatca tcgggcggcg gtgctttgat aaccggcgct tcgccttgca
    48721 gataacgatt aatatcgcgc agcaacgcct gcggcccttt ccaggtttca aagacgacct
    48781 gggctatttc acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt
    48841 agcgtagcag agactcatct tcaatcagct gctgctcaaa cagcatgccg gattcaaagg
    48901 cattcaggtt gagcatgcgc tggcaaaagc cgtgaatagt aaagactgcc gcttcatcca
    48961 tctgccgttc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca
    49021 gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg cgcaactcgt
    49081 ggatattgct acggatacga ccgcgcaatt ctgccgtggc agcctcggta aaggtgacca
    49141 ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc ggaaccgcct agtccaagta
    49201 acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa
    49261 tcaggcgctc accctgtaag ggcaagcgca aaggatctag tgtctcggcg acatcactca
    49321 ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc
    49381 cagccttcag ggtgtacata ttcggctttc ccgttctggc tgccggaaat ctgcgacaga
    49441 atagccatgc cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc
    49501 gtcagcagtt ttatctgggc cacgatttta tcacgcgaat cgaagcgcat attgccgcga
    49561 tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc
    49621 tgggtaatta ccgcctgctg gatttgcgca aactcatctg gcttcatcgc tcgcaatttt
    49681 gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca
    49741 ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac
    49801 acggcatagc ccaattgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc
    49861 tgccccaaca gagagctata ggctgagctg gtgtattcat cgtagccagt cggtacaaat
    49921 accgctgcca gtgcggagtc ggtgctgtta ccggcttttt caaagatgac ggattgtttt
    49981 ttatcgacca ctacatcttt gtttcgacac cactctgaac catcagcgcc caactgtttt
    50041 tgcacatcgc gtgccagcgt tgttgcctgg gcctcggtca tgttgccgat aaccataaac
    50101 tctggtcgag cccctgattt taaggcgtcg cgataggcca gcacctcttt caacgtaatg
    50161 gagggcaaaa ttttacgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc
    50221 gcgggcataa tcgcctgctc aaacgcttta cccttttctg cggaatccat catctggtta
    50281 taccaggact tcgcctgctc aagctgatct tccgtggcgg tatagctaaa gtagccttcc
    50341 agcaatgcct ggaacagctg cggcagacgc tgggtgtaac cattagcatt aaccataagg
    50401 ccgttgttag cgttggtgga aaaacttatg ccaccaaccg acgcctggtt gcttaactga
    50461 tcaagcgcca gccctgcgag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg
    50521 tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggctc gctggcaaaa
    50581 taacggcttg gcgcatacac cacgcgcaga ttcgactcat caacaatcag ctctggatgg
    50641 tcgtatttct tctctgactt aatcagcgag aaatcatcag gaatataagg gttaagctct
    50701 ggcaaagaga gcgcaatgtc ggcggctttt ttctgccagt cggcgaaagt ttgtgcgctg
    50761 attttatcga cctgatacgg cgcatcgaca aagtaagccg ttttgttgtg cggctctttc
    50821 gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccagacg ttcctttact
    50881 gctttagcat cgtaccgatc ggcaatattg actgcatcca gcgtatgctc aacaggaacg
    50941 cgaatcatgg tatctgccag ccattcgacg taatccatat cacgggtgat cgacggataa
    51001 cggaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc aatgcctttt
    51061 tcacgtaaca gattgagata gctaaaaatt gccgccacaa cctgatcgcg attagccagg
    51121 cctttatcgg ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga
    51181 tcggagttgg cgctaatgcc ctcaactaat ccctgctttt gcagccagtc agaaagtgta
    51241 cctgggctgc gattgccaat cagataggta atcaattcat cggttttact acggaacttc
    51301 gctgagttgt tatcgatgcg aaactcaacg cgcaacactt tacgcggcag cgcagggacg
    51361 taatgaatga taatgccctt ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt
    51421 ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccatttttgc caactccggc
    51481 agcggtttat tgctgtaaat aaccgccttc atcagattgg cggaatagta cttctcgtgg
    51541 aaatctttca gcgcctgctg caccggatta ccaggtttgt cgcttaaagt ttcgaggtta
    51601 ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgcgcc
    51661 atgcgcatcc cgtcacgcgt acgcgccatg gttaattcgg cattcactgc attacgttca
    51721 cgttcggcgt atttcttgtc gagcaagggt tctgcaatag cgtccgccag gcggtctacc
    51781 gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggtgccgtg
    51841 ctggcattgt gactgccgcc gtgcattttg agatattcgg ccagactgtc agcctgcggg
    51901 tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc ctggtacgcc
    51961 tcgggatctt ccagcgaccc aacgggcacc accagcgccg agagcgattt aaccgcctgc
    52021 ggatcagaaa ccagcaagac caccataccg ttatccagac gtatagcctg atactggcgg
    52081 ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga
    52141 cttaagggtg cccaaagggc aacaaacaac aataatgctt tgaaccaggt gctgcggggc
    52201 attcacggac ctcataatca actttatttt ctggccagat tcaacaggtt aacgcaaatc
    52261 atctgccaga atttaatctt gtgctgcacg agtcagccta tgtctatata tccatcagtc
    52321 cgtgactggt gcgcatcata atgtaagcga atgaattgcg caatttttat acagcactca
    52381 tgactgatta aagcgaaaca gcggtaatag gaaacgttgc gactgttcaa cgatggcctc
    52441 cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc
    52501 acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgcaacgt
    52561 ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc
    52621 gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc cctcaatcag
    52681 ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc ggaaaacgcc actcaccgtc
    52741 tttgcgtaga aaaaggcgac tttcgccatt accaccgctg gcacagtaga caaggtgttc
    52801 cagccaaagt tgcattccct gcgccacact taataaagag ggacgccagc gcaacaggcc
    52861 atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcaat
    52921 ttccatactc tgccccggct ggcgacaggc aatgactctg tcggcaagct gctgcatctc
    52981 ctggcactgt gtttcccaga aaatttcacc aaaagcgcca tacggtaaat cccctgccgc
    53041 ccggaagcgg cggaacaagc gttcggcatc atcctgctca accagtgcat tcaataactg
    53101 ctgattgatt tgataacggc taagtccttc cagaataaat ggctcggtgt ctgggatttc
    53161 gctgtcttca gtacggaagt tcacctgcaa acgcatctgg aagaacgccc gcaccggatg
    53221 tgcccagaat cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa acggcagcgg
    53281 ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc attcacgagc
    53341 atagctttgt cgttcgcctg gctggtagtt ttgtggatca aacggcatcc gggtatggag
    53401 gcaagtaaga tgcgctttta cccttgcctc gctttcatca cagttgagcg cttcatcgcc
    53461 cggtagataa tgactttgcc cgatgtagtc gatcagttcc tgcaccagta ccgacgggaa
    53521 acgttcactg ttatcctgaa tggaacgccc gatatagctg atatagagtt tttgctgcgc
    53581 ggaaattaac gcttccagga acaggtagcg gtcgtcatcg cgacggctac ggtcgccacg
    53641 cttcggtttc tggctcatca ggtcaaagcc caatggcgca agctgacgtg gataaacgcc
    53701 gtcgttcatt cccagcaggc aaaccacttt gaacggaatt gaacgcattg gcatcagagt
    53761 acaaatgtta accggtccgg cgagaaaacg ctggctgata cgttcttgat ccagacgctg
    53821 cgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc
    53881 ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atcgccgctt cggtttccgc
    53941 atccggcagg aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttctagcgg
    54001 acgctcctgt gccagcccgc gacgccagat gtttagctgc attagcagtg aagccagatg
    54061 ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc
    54121 gccctgcgcg ctctccatcg cgtagcccag caacatacgc gtcaggccaa atcgccaggt
    54181 gtgttgtccg gtggcgggga gttccagctc gcgaacgttg tcgtcatcta tcccccaacg
    54241 aatgccggat tcgttgaccc actggcgtaa ataacgcagc ccttcttcgg tgatgtcaaa
    54301 ccgcgccgcc agcaccggca catccagcag cgccagcaca tcctctgaca caaagcgact
    54361 gtcaggcagt gataacaggc tgataaacgc ttccagtacc ggatgtgact gccgcgcacg
    54421 acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcgctaccaa acactgcctg
    54481 aataaacgga ctgtagctgt cgatatcagc caccatcacg atgatgtcgc gcggagtaag
    54541 tgtcgcgtct tcctccagca tcgccagcaa gcgatcgtgt aaaacttcaa cttcacgctg
    54601 cgggctatgg caaacgtgga aggtgatact gctatccagt ggatcaagcg ggcgtttgtt
    54661 atcgctacgg gaaaactctt cgatgttcac acccgcaacg gcgcggtttt ccagttccag
    54721 aatgtcagac tgaatgttat gcagcaggtt atctggcgtg acatcgacaa aggcgtccag
    54781 ctcctggctg ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa gcttacccca
    54841 tgaagccagc agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga gctgcccggc
    54901 attttcgcta tcacgaaata gcggcaattc gcgatcttca aaactgtgtc gacgctgacg
    54961 ggtcagcagt ttcgccagat aagcaggatc tttaatatcg ccccagtaat aacggcaggg
    55021 gttggtaaac aggagatgga tttcaatatg tttacccagc gcctgtagcg cctggagata
    55081 aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc ccggcgggca
    55141 ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattggcgc ggtgccaacg
    55201 cggttgtccg agttcatggg tatattccac cagcgccttc cacaacggcg cttgccaggc
    55261 ctgtgcctct cccagccctt caaccaaatg tcctgtttcc cagtgtgcca gccagtccgg
    55321 acgatagacc agatactggt caaacaggtc cgccgctttt gaggaaagct ggaacagttt
    55381 tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccag
    55441 caattgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcactctc
    55501 tttggggatc tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg
    55561 aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgcgc
    55621 cataccggta ctttgcacca gaatcatctc tggttcgaaa ggatcgtcca gccgttcgcg
    55681 ttcgacaata aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct
    55741 taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc
    55801 tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg
    55861 cattcggttg acctgccagt tggcaggtgg cgaaatcgcg cgcagttgcg tttgctgcca
    55921 accatgccgc cagagctgtt ggtactggtt tctgctggcg agactgttca ttaatgttcg
    55981 ctggataccc gataacgcag tgacaatcat caccatcagc accatcgcaa ccattacctc
    56041 cggcaggcta aagccttgtt gattcttcag ggaagctgac ataatgcccc ctctttcagc
    56101 ggacaaaaat cgctccagcc gcgtggcgaa aaaacaattt ttccatcaat gacttcgcct
    56161 gttcgccaca acgaaacgcc ttcataaccg gcaatcaata aggcttcatt atctgccagt
    56221 aaacgcaaac aaacctgggc atcggttcca gcgtactgcg agcactgaac tgctggctgc
    56281 gtctgccagg agtgcatttt tccccacgcc agcgccgact gaacgatggc ctggcggcgc
    56341 aatgactgac tttccatgct cacgcgagag gcaaaactgc gatcctgctg actcattcct
    56401 tgcaatagca agctacccaa aaccagcagc atcaggacca gagccagtga cgaaacacct
    56461 ttttcgcggt tcacaggttg aatcctgtca cgctatagct ggcatccacc acggtttgcg
    56521 gttcagactt actggcagca cgcatattaa ccgtcaacac cggcgagaag ccgctgacat
    56581 cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc cggattagtg actttatccc
    56641 agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac
    56701 gaaatccaat ctggtcggac tcttttaccg gttcgcgatc ccagataccg ttactgttcg
    56761 catcccactg cacaatgata cagtcaccct gtccgacaat ttccagccct tcgccggtac
    56821 agctgccatg acaataaccc gccctctgga gatgcttcgc gacggtaaat acccgcagcc
    56881 agatttcatc ttccagcgcc agcttacggg tgcccgttaa actttcacgc tgtaacgcag
    56941 gcagaaagcg tgccgctccc agcaacaata cgctactgat cgccatagca atcaacactt
    57001 ccagcagaga aaaaccttgc tcttttacag gcatccttct gtttctcctt gctgacaaag
    57061 ccggagtctt ccccacggcg aaaccaccag ccaccactcg cccgttgagt ttttgaagcg
    57121 aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc
    57181 gctcatttcg acttcgggcc agcgtggcac aaagaccaat ggtgaactgc catgacaggt
    57241 attggcccca gcagcggaac tcacaaggca ccataacgtc ccctccctga taacgctgat
    57301 actgtggtcg cggttatgcc agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc
    57361 ctggctggcg gtttgccata gccgttgcga ctgctgccag tattgccagc catagagtcc
    57421 acttgcgctt agcatgacca aaatcagcat cgcgaccagc gtttcaatca gcgtataacc
    57481 acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa aaaagacgag ggccagtttc
    57541 tatttcttcg gcgcatcttc cggactattt acgccgttgc aggacgttgc aaaatttcgg
    57601 gaaggcgtct cgaagaattt aacggagggt aaaaaaaacc gacgcacact ggcgtcggct
    57661 ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga tgtggatcgt
    57721 agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt ttacgtttga
    57781 taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt tgatccatat
    57841 gattgctgta caggtgcgtg tcgccccccg tccagacaaa atcgcccact tccagatcac
    57901 actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttaaac ggcaggccga
    57961 ggaaaacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct gccacataga
    58021 actggaagaa cgcatggcac ggtgccagcg ccattttatc cagttcgcct acgttccacg
    58081 ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag ctggttcagt accgtagtga
    58141 tctggtcaat atgacgacca tccggcgttg gccaggcgcg ccactgttta ccatacactg
    58201 gcccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg ttttcgtgta
    58261 gataagcaat gttagtgtcg ccctgcagaa accacagcag ttcatggatg atggaacgca
    58321 ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa cgcatctgat
    58381 gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg ccttcgtcga
    58441 gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg tgttgctgtg
    58501 ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc gggatggaaa
    58561 gaatttgtcc catactgatg tactgcaccc aggcaccagt aaactgcgcg tcgggctggc
    58621 ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat caggaacaaa cctgagacag
    58681 ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc actacacctt
    58741 ccagcaacag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa gtatcgaaaa
    58801 tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg gagccaggga
    58861 acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg ttaataaagt
    58921 tgcccagacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa tcagagacct
    58981 ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata acgccaatca
    59041 ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc ggatcggcca
    59101 taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg aagacgccga
    59161 ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccgctg cccggacgat
    59221 tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac atcaggccgt
    59281 accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc ggaaaatgca
    59341 gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag agaacagcga
    59401 ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc acaaggggac
    59461 ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat aaccctccgc
    59521 gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatgg cgaacttcgg
    59581 tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca taatcaatgc
    59641 gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga tggcgatacc
    59701 caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg cacaaacgga
    59761 gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt aacattgcag
    59821 gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg gccagaatgt
    59881 attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc agatgcggca
    59941 gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg ggaatttcgt
    60001 aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt gcttcatcaa
    60061 cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta gcggcattag
    60121 cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc gtaatgcgaa
    60181 tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc agctgcttat
    60241 ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt tgcagcatcc
    60301 cctgatactg cgccacctgt tcttcttccg acgggaaacc actttgcagc atgaatggga
    60361 tttcagtgcg ataaagaccg atgccatcaa tacggctgcc cagtttttct tcatgttccg
    60421 ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac tgcgcgggta
    60481 aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt agccgctgat
    60541 attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag ccatcaacga
    60601 tcagcgtcct acgatgcagc accgaaggct gaatatccgc gcccatcacg gtagggatcc
    60661 ccagcgcacg taccatgatc gcagcatggg agttggctgc gccatctcgc acgacaacac
    60721 cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca tctgccacca
    60781 gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga tgaaacagca
    60841 atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta tcgcttagcg
    60901 cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct gccaccgagc
    60961 ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc aggtgcgagt
    61021 aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc ggcaaagcgt ttgctgtagc
    61081 ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt tccagagccg
    61141 gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca tcctgccagc
    61201 cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg gattcgcgtc tggcgatatt
    61261 gcccaaacaa ggcagtcaac tgcgactgag aaagaatagc tgccatctgg gtggcaagcg
    61321 tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca
    61381 gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt
    61441 cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct gcaaggttta
    61501 tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg agcgttacag
    61561 tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa cgtcgatcat
    61621 gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg tcggtaacca
    61681 gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact atttcgcgca
    61741 ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca gatgcgtttt
    61801 gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct ttcattaccc
    61861 tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag taacttaccc
    61921 atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct gcatcgccgc
    61981 tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc ttcgtgtccc
    62041 aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa gcaaggattc
    62101 gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc gcctgctctg
    62161 cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga ccaaatcgcc
    62221 gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt gggcggtagc
    62281 catcgtcatc aatcaccgga ctacctcaat ataaagcttt atatatgaat gattgtttca
    62341 tactccagga agacggtaaa ccactctctg cagggcatta cacactaata acaattgaat
    62401 aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg tttttattca
    62461 ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa cccggagcat
    62521 tctgaataag cccgatatag acattttaaa atattcatta tcagtcaatg cattacatgt
    62581 aattttagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat ttacgggttg
    62641 gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt aagcactgaa
    62701 ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag tagtggaggt
    62761 ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt ttattttcag
    62821 tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc aacctaaaaa
    62881 gcaatataaa tcagagaatt gaacaacgca tgtggaaaaa gttacactgc gaatattcgg
    62941 cacataattg ctgtttgttt tttaatcaag gtatcatgac atgtcccaac ctcgcccact
    63001 gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac tttctggtta
    63061 tacattggga gaactggcgg cacttgtcgg gctggttacg ccagagaatt taaaacgcga
    63121 taaaggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag ggagtaaacc
    63181 tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg atagtcttgg
    63241 tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggcaata gcggggtgac
    63301 ctgggaaacc agccacgtgc gccacaaact caaacgcgta ctgtggatac cggttgaagg
    63361 cgagcgcagc atcccgctgg cgcagcgtcg cgttggatca ccgttgctgt ggagcccgaa
    63421 tgaagaggaa gaccggcagc tacgcgaaga ctgggaagaa ttaatggata tgattgttct
    63481 cggtcaggtt gagcggatta ccgctcgtca cggggagtat ttacagatac gaccgaaagc
    63541 agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg ggcgaacgga ttctgacgct
    63601 gccacgcggc ttttatttga agaagaattt caccagtgca ctactggccc gtcattttct
    63661 gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc gcttcatttt
    63721 tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc ctggcttgcg
    63781 ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat tttcctttct
    63841 ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct ggggttggcg
    63901 ggagccatgg ttatgcgtct ggcgctgctg gcatcaatcg cctgggttac gcgcctgaca
    63961 aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgacttgat tctgcttctg
    64021 ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat tgaaggtgaa
    64081 gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca gattatgctg
    64141 ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc agatcacctg
    64201 tttattatga tggcagccgt ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg
    64261 attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt
    64321 ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa aggttacatc
    64381 tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg caacaaaaag
    64441 aacccgctct gatactccgt acgctctcct gcgggagggc gtactcactc ctgttaccct
    64501 cacagtttaa gattttcctg ctttcaagat atataacgtc agtttataaa cagactatta
    64561 tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata atttctgcag
    64621 tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg accaaagatg
    64681 gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg ctggtgagtt
    64741 atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg
    64801 aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt tcccttttcc
    64861 cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa cggctatgca
    64921 atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg gcacgatgac
    64981 gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca
    65041 gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca
    65101 agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt
    65161 aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca tccgcccgga
    65221 tcaggcgctg gatcggaaga atatccgcga agcgttgcat gacagcctca agcgtctgca
    65281 gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccaacca actgcttcgg
    65341 caaactcggt tatagctgga cagattctgc gcctgcggtt tcgctgctgg atacgctgga
    65401 cgcactggca gagtaccaac gagcgggaaa aattcgttat atcggcgtgt cgaacgaaac
    65461 tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc cgcgcattgt
    65521 caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt
    65581 cagccagtat gaagggatcg aactgctggc ctattcgtgc ctgggtttcg gcacgctgac
    65641 cgggaaatat ctcaacggtg caaaacccgc tggcgcacgt aatacgctct ttagtcggtt
    65701 cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag
    65761 acgtcatggc ctggaccctg ctcagatggc gctcgcgttt gtacgccgtc aaccgtttgt
    65821 tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca tcgaaagttt
    65881 gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtgcatc aggtttatac
    65941 ttatccggca ccataagatt aacgccggtt aaaaccggcg ttaaatatta atgacggcgc
    66001 tgccagatcc acagcgccgt tattgccagc gcaaacagcg caccgaagcc aatgccaatg
    66061 ggcacgaccg ggatgcctat cattaccgcc agcgagtaaa tgcccagcat caacaacatg
    66121 gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac gcttttttta
    66181 ccccgctcct gtagcaacgc attgagcgga acgacaaaaa agccccccat cacgccaatc
    66241 agcatcaaca aggcataggc tggcagcagc tcgtgttgca gggaaaaaat cagtaccacc
    66301 acgccaatca aaatcccggc tggcatacag cgtgacacgg tttccagcgt aactaacttc
    66361 gctgccgcac ctgcgccaac cacgatacca atcgctacca tcgcgttgag ataggtgggc
    66421 gtagcgttat cggtaatgcc cagcgccacc ggtacccaca gcaccaacag gaaacgcagc
    66481 gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt ttcaccattg
    66541 cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgttgatgag attccaggac
    66601 tgccccggac gcgccgccgc cagtttggga atgtagatat tggcaacgac cgcaccaccg
    66661 taggccagtg cgcatgcggc cagggcgacg aggacatgcc agtcagccag cacaccaccg
    66721 gctacggaac cgagcaaaat cgccgctatg gtagaagctt ccattaaacc gttagctttc
    66781 actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct
    66841 gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa gcagatactg
    66901 gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta
    66961 tccgccacct gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc
    67021 aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac
    67081 agtagggcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc
    67141 ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc
    67201 tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt
    67261 cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg
    67321 ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt
    67381 gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag
    67441 ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg
    67501 gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag tgtcatacca
    67561 gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta cacctggctt
    67621 ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc
    67681 ttcgatacca gggaccgaca acaggcgcgc atccattcct ggtagaatac gccctaccgt
    67741 accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt cggtcacgcc
    67801 gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg
    67861 taatttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcgaa
    67921 acgcgcgtag tgaccgagga aagtcgaggt gccgaacaac acggtgcaac tgcggtcata
    67981 caccaactcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc
    68041 tgtaagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc
    68101 cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacattcgc
    68161 cagaatgctt ttatggctat ggacgacgcc tttcggatgg ccttcagaac cggaggtaaa
    68221 aaggatcagc gcctcttctt ccggctgctg tttaacctgt gccagacgcg gcatcagcaa
    68281 atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat cttccagata
    68341 gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa
    68401 ctggcgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg tcagcccttt
    68461 taccccggca gtgtagttca tcattgcggg catgcggcga cgggcgatgg ccccaaaaat
    68521 cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact
    68581 gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc
    68641 tggggtaaag ttgacgtctt cgacacattt cttcccggct ccgaagcggt acattgcact
    68701 cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc attcgcgctt ccatcattat
    68761 ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat
    68821 cgccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac
    68881 cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac
    68941 cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg tagattttca tcagcgagcc
    69001 tgtcgtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt ccaccagacg
    69061 taccagatgt ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga
    69121 tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa acactggacg
    69181 cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat taggcgtaat
    69241 tagaacgcgc tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac
    69301 acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta
    69361 aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg
    69421 aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg
    69481 aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacga cgactcttcg
    69541 ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta
    69601 ctgttacaga ttgatgaccg gcaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc
    69661 atgtgctcaa cccgaagttg acttcaccta tcaatacctc tgggatcacc actttagcaa
    69721 cctgaagcca aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag
    69781 gtttgcaatc tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa
    69841 acacgccacc ccttgaacca acgggcgttt tccgtaacac tgaaagaatg taagcgttta
    69901 cccactaagg tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag
    69961 tcgccaccgt ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg
    70021 ctgtgcatag tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg
    70081 cgcagcagac cactgaaacg gtcggtctgg tcgttggtga tgtttccgat ccgtttttcg
    70141 gtgcaatggt gaaagcggtc gaacaggtgg cttatcacac cggtaatttt ttattgattg
    70201 gcaacggtta ccacaacgaa caaaaagagc gtcaggccat tgagcaactg atccgccatc
    70261 gctgtgctgc gttggtcgtc catgccaaaa tgatcccgga tgctgattta gcctcattaa
    70321 tgaaacaaat gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt
    70381 gtattgctct ggacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag
    70441 gtcatacccg cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc
    70501 tgcaagggta ttacgatgcc cttgctgaaa gtggtattgc ggccaatgac cggctggtga
    70561 catttggcga accagacgaa agcggcggcg aacaggcaat gaccgagctt ttgggacgag
    70621 gaagaaattt cactgcggta gcctgttata acgattcaat ggcggcgggt gcgatgggcg
    70681 ttctcaatga taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg
    70741 tgctggtgtc acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtgacga
    70801 tggcgaccca ggctgccgaa ctggctttgg cgctggcgga taatcgccct ctcccggaaa
    70861 tcactaatgt ctttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg
    70921 aggcaagtca tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat
    70981 tcttcgatgg tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct
    71041 ggtaacagcg gacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca
    71101 tgcagtacca gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc
    71161 ccttcctgct gggtaaagac atcgcccgat tcacataacg gtccggcgac gacggtttcc
    71221 accgttggcg cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa
    71281 ctaccgtaca ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg
    71341 cggctcccca tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc
    71401 aggaagcgac ccggttcaat ttccagtttc acagggtggc ccaaatggcg ggcgatttgc
    71461 tcacgcgcgg cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt
    71521 tgataaggaa cagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg
    71581 atgacctgac gcaccatagc accacacacc tgttccagat gggcataatc aacgccagaa
    71641 ccaatgtgca tgtgaatgcc gaccagctgc agatgatgac gttgtatcac gtccagtgcg
    71701 gcgggcagat cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt
    71761 tggctatgtc cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa
    71821 acctggccca gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca
    71881 ctgacgcgtt caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg
    71941 tgcgtttgcg gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa
    72001 tccactttca cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc
    72061 tgtgcaaagc gcaccacatc aaactgtttc agcgctgcaa tctgccgacg aataatttgc
    72121 gcatcgtaga cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg
    72181 gtgagatcgg tatcggtgct gaacagtgaa tgtggcataa caaactccag ataagtgctt
    72241 ttttatgatt acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgagtctatg
    72301 caaaaatgat atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt
    72361 catattgaaa tttttcatgc ggtaatgacc gccggaagcc tgactgaggc ggcacacctg
    72421 ctacacacct cacagccaac cgtcagccgc gaacttgcgc gctttgagaa ggtgatcggg
    72481 ctgaaattgt ttgagcgcgt acgtgggcga ttacatccta ccgtgcaagg actgcgtctg
    72541 tttgaagaag tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt
    72601 ctgcgcgagt ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct
    72661 tttttaccgc agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc
    72721 gtgccccagg aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga
    72781 ctcactgaaa cgctccatac gcctgcggga acagaacgta ccgaattact ctctttagat
    72841 gaagtgtgtg tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat
    72901 gattttcagg gtgagaacta catcagcctt tcccgtactg acagctatcg ccagttgctg
    72961 gatcagctat ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc
    73021 gcgtcagtct gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc
    73081 gcactagatt atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc
    73141 accgtca
//
LOCUS       DACAPB010000003        66936 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.003, whole genome shotgun sequence.
ACCESSION   DACAPB010000003 DACAPB010000000
VERSION     DACAPB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.003"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(217..1134)
                     /gene="lpxC"
                     /locus_tag="IT904_000146"
     CDS             complement(217..1134)
                     /gene="lpxC"
                     /locus_tag="IT904_000146"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAP3189986.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(1235..2386)
                     /gene="ftsZ"
                     /locus_tag="IT904_000147"
     CDS             complement(1235..2386)
                     /gene="ftsZ"
                     /locus_tag="IT904_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP3189987.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(2447..3709)
                     /gene="ftsA"
                     /locus_tag="IT904_000148"
     CDS             complement(2447..3709)
                     /gene="ftsA"
                     /locus_tag="IT904_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP3189988.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(3706..4536)
                     /gene="ftsQ"
                     /locus_tag="IT904_000149"
     CDS             complement(3706..4536)
                     /gene="ftsQ"
                     /locus_tag="IT904_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAP3189989.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(4538..5458)
                     /locus_tag="IT904_000150"
     CDS             complement(4538..5458)
                     /locus_tag="IT904_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP3189990.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(5451..6926)
                     /gene="murC"
                     /locus_tag="IT904_000151"
     CDS             complement(5451..6926)
                     /gene="murC"
                     /locus_tag="IT904_000151"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP3189991.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(6980..8047)
                     /gene="murG"
                     /locus_tag="IT904_000152"
     CDS             complement(6980..8047)
                     /gene="murG"
                     /locus_tag="IT904_000152"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP3189992.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRVARA"
     gene            complement(8044..9288)
                     /gene="ftsW"
                     /locus_tag="IT904_000153"
     CDS             complement(8044..9288)
                     /gene="ftsW"
                     /locus_tag="IT904_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAP3189993.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(9288..10604)
                     /gene="murD"
                     /locus_tag="IT904_000154"
     CDS             complement(9288..10604)
                     /gene="murD"
                     /locus_tag="IT904_000154"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP3189994.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(10607..11689)
                     /gene="mraY"
                     /locus_tag="IT904_000155"
     CDS             complement(10607..11689)
                     /gene="mraY"
                     /locus_tag="IT904_000155"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP3189995.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(11683..13041)
                     /gene="murF"
                     /locus_tag="IT904_000156"
     CDS             complement(11683..13041)
                     /gene="murF"
                     /locus_tag="IT904_000156"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP3189996.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(13038..14525)
                     /gene="murE"
                     /locus_tag="IT904_000157"
     CDS             complement(13038..14525)
                     /gene="murE"
                     /locus_tag="IT904_000157"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAP3189997.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(14512..16278)
                     /locus_tag="IT904_000158"
     CDS             complement(14512..16278)
                     /locus_tag="IT904_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAP3189998.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(16294..16659)
                     /gene="ftsL"
                     /locus_tag="IT904_000159"
     CDS             complement(16294..16659)
                     /gene="ftsL"
                     /locus_tag="IT904_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP3189999.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(16656..17597)
                     /gene="rsmH"
                     /locus_tag="IT904_000160"
     CDS             complement(16656..17597)
                     /gene="rsmH"
                     /locus_tag="IT904_000160"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP3190000.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(17599..18057)
                     /gene="mraZ"
                     /locus_tag="IT904_000161"
     CDS             complement(17599..18057)
                     /gene="mraZ"
                     /locus_tag="IT904_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP3190001.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIYHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(18659..19663)
                     /gene="cra"
                     /locus_tag="IT904_000162"
     CDS             complement(18659..19663)
                     /gene="cra"
                     /locus_tag="IT904_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAP3190002.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(19745..19831)
                     /locus_tag="IT904_000163"
     CDS             complement(19745..19831)
                     /locus_tag="IT904_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAP3190003.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(19843..20334)
                     /gene="ilvN"
                     /locus_tag="IT904_000164"
     CDS             complement(19843..20334)
                     /gene="ilvN"
                     /locus_tag="IT904_000164"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP3190004.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(20337..22061)
                     /gene="ilvI"
                     /locus_tag="IT904_000165"
     CDS             complement(20337..22061)
                     /gene="ilvI"
                     /locus_tag="IT904_000165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAP3190005.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            22181..22225
                     /locus_tag="IT904_000166"
     CDS             22181..22225
                     /locus_tag="IT904_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190006.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(22380..23324)
                     /gene="leuO"
                     /locus_tag="IT904_000167"
     CDS             complement(22380..23324)
                     /gene="leuO"
                     /locus_tag="IT904_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAP3190007.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKKASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            23989..24075
                     /gene="leuL"
                     /locus_tag="IT904_000168"
     CDS             23989..24075
                     /gene="leuL"
                     /locus_tag="IT904_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAP3190008.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRQVSGIQH"
     gene            24168..25739
                     /gene="leuA"
                     /locus_tag="IT904_000169"
     CDS             24168..25739
                     /gene="leuA"
                     /locus_tag="IT904_000169"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAP3190009.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITEYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            25739..26830
                     /gene="leuB"
                     /locus_tag="IT904_000170"
     CDS             25739..26830
                     /gene="leuB"
                     /locus_tag="IT904_000170"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAP3190010.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            26833..28233
                     /gene="leuC"
                     /locus_tag="IT904_000171"
     CDS             26833..28233
                     /gene="leuC"
                     /locus_tag="IT904_000171"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAP3190011.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            28244..28849
                     /gene="leuD"
                     /locus_tag="IT904_000172"
     CDS             28244..28849
                     /gene="leuD"
                     /locus_tag="IT904_000172"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAP3190012.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWAMTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(28898..30076)
                     /gene="setA"
                     /locus_tag="IT904_000173"
     CDS             complement(28898..30076)
                     /gene="setA"
                     /locus_tag="IT904_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAP3190013.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDNQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFNSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(30178..30309)
                     /gene="sgrT"
                     /locus_tag="IT904_000174"
     CDS             complement(30178..30309)
                     /gene="sgrT"
                     /locus_tag="IT904_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAP3190014.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            30398..32053
                     /locus_tag="IT904_000175"
     CDS             30398..32053
                     /locus_tag="IT904_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAP3190015.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      32087..32186
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            32217..33200
                     /gene="thiB"
                     /locus_tag="IT904_000176"
     CDS             32217..33200
                     /gene="thiB"
                     /locus_tag="IT904_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAP3190016.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGRGLLLWMQKVYGDDTPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            33176..34786
                     /gene="thiP"
                     /locus_tag="IT904_000177"
     CDS             33176..34786
                     /gene="thiP"
                     /locus_tag="IT904_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAP3190017.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGLNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYNMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            34770..35468
                     /gene="thiQ"
                     /locus_tag="IT904_000178"
     CDS             34770..35468
                     /gene="thiQ"
                     /locus_tag="IT904_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAP3190018.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVECGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(35582..36346)
                     /locus_tag="IT904_000179"
     CDS             complement(35582..36346)
                     /locus_tag="IT904_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP3190019.1"
                     /translation="MQALLEHFITQSTAYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIEIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(36432..37310)
                     /gene="araC"
                     /locus_tag="IT904_000180"
     CDS             complement(36432..37310)
                     /gene="araC"
                     /locus_tag="IT904_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAP3190020.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            37649..39349
                     /gene="araB"
                     /locus_tag="IT904_000181"
     CDS             37649..39349
                     /gene="araB"
                     /locus_tag="IT904_000181"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAP3190021.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGGRAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            39360..40862
                     /gene="araA"
                     /locus_tag="IT904_000182"
     CDS             39360..40862
                     /gene="araA"
                     /locus_tag="IT904_000182"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP3190022.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSISVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            41062..41757
                     /gene="araD"
                     /locus_tag="IT904_000183"
     CDS             41062..41757
                     /gene="araD"
                     /locus_tag="IT904_000183"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP3190023.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            41832..44183
                     /gene="polB"
                     /locus_tag="IT904_000184"
     CDS             41832..44183
                     /gene="polB"
                     /locus_tag="IT904_000184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAP3190024.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGIAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            44348..47254
                     /gene="rapA"
                     /locus_tag="IT904_000185"
     CDS             44348..47254
                     /gene="rapA"
                     /locus_tag="IT904_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAP3190025.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            47266..47925
                     /gene="rluA"
                     /locus_tag="IT904_000186"
     CDS             47266..47925
                     /gene="rluA"
                     /locus_tag="IT904_000186"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAP3190026.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(48042..48857)
                     /gene="djlA"
                     /locus_tag="IT904_000187"
     CDS             complement(48042..48857)
                     /gene="djlA"
                     /locus_tag="IT904_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAP3190027.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            49112..51466
                     /gene="lptD"
                     /locus_tag="IT904_000188"
     CDS             49112..51466
                     /gene="lptD"
                     /locus_tag="IT904_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAP3190028.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            51519..52805
                     /gene="surA"
                     /locus_tag="IT904_000189"
     CDS             51519..52805
                     /gene="surA"
                     /locus_tag="IT904_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAP3190029.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            52805..53794
                     /gene="pdxA"
                     /locus_tag="IT904_000190"
     CDS             52805..53794
                     /gene="pdxA"
                     /locus_tag="IT904_000190"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAP3190030.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSRAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPAQLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            53791..54612
                     /gene="rsmA"
                     /locus_tag="IT904_000191"
     CDS             53791..54612
                     /gene="rsmA"
                     /locus_tag="IT904_000191"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP3190031.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            54615..54992
                     /gene="apaG"
                     /locus_tag="IT904_000192"
     CDS             54615..54992
                     /gene="apaG"
                     /locus_tag="IT904_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAP3190032.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            54999..55841
                     /gene="apaH"
                     /locus_tag="IT904_000193"
     CDS             54999..55841
                     /gene="apaH"
                     /locus_tag="IT904_000193"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAP3190033.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(55919..56398)
                     /gene="folA"
                     /locus_tag="IT904_000194"
     CDS             complement(55919..56398)
                     /gene="folA"
                     /locus_tag="IT904_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAP3190034.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(56590..58452)
                     /gene="kefC"
                     /locus_tag="IT904_000195"
     CDS             complement(56590..58452)
                     /gene="kefC"
                     /locus_tag="IT904_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAP3190035.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(58445..58975)
                     /gene="kefF"
                     /locus_tag="IT904_000196"
     CDS             complement(58445..58975)
                     /gene="kefF"
                     /locus_tag="IT904_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAP3190036.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(59083..60414)
                     /locus_tag="IT904_000197"
     CDS             complement(59083..60414)
                     /locus_tag="IT904_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3190037.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(60473..60760)
                     /gene="fixX"
                     /locus_tag="IT904_000198"
     CDS             complement(60473..60760)
                     /gene="fixX"
                     /locus_tag="IT904_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAP3190038.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(60757..62043)
                     /locus_tag="IT904_000199"
     CDS             complement(60757..62043)
                     /locus_tag="IT904_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP3190039.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLVREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKIDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(62094..63035)
                     /locus_tag="IT904_000200"
     CDS             complement(62094..63035)
                     /locus_tag="IT904_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP3190040.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(63050..63820)
                     /locus_tag="IT904_000201"
     CDS             complement(63050..63820)
                     /locus_tag="IT904_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAP3190041.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            64293..65807
                     /locus_tag="IT904_000202"
     CDS             64293..65807
                     /locus_tag="IT904_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAP3190042.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            65838..>66936
                     /gene="caiA"
                     /locus_tag="IT904_000203"
     CDS             65838..>66936
                     /gene="caiA"
                     /locus_tag="IT904_000203"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAP3190043.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEM"
BASE COUNT        16199 a        18595 c        16978 g        15164 t
ORIGIN      
        1 gatcgtattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgca gtccacagca
       61 cgaatgaaga taagttgtgc ggaaaagtgc ttaagaaaac agcgttcgca ccgtttttta
      121 tagctcttat aaaattcaaa aaaaaacgac tggttcacct ggccggagag agtgccagat
      181 ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacag ctgaaggcgc
      241 tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg
      301 tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt
      361 aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt
      421 gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc
      481 aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc
      541 gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc
      601 ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat
      661 ggtgaaatcc agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc
      721 gacacgaaca gtctctttga tgcgaacaaa ttttttggcg cagttcaact cgtcgatacc
      781 ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg
      841 cgcgttaact tcgataacaa tgttatcgat gcccaggccc gcgagagcag cattgaggtg
      901 ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc
      961 acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata
     1021 gatgaccccg gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg
     1081 taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt
     1141 atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca
     1201 caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta cgcaggaatg
     1261 ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcacgactt
     1321 tagcaaccgg cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca
     1381 tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc
     1441 cgatacctgt cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag
     1501 taccgataac cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt
     1561 cgaactcatc cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc
     1621 cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg
     1681 cacggtcttc accgctcgcc acgccagaac ccatcattgc gtagcccatt tcagacatta
     1741 cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcggcga
     1801 tgccctgaac agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga
     1861 taccgcggcc cagaactttc agcagtttgt cgttcgggat agtaatcaga gagtccacat
     1921 gcttggacag ttcagtgatc ccctgctccg cgaatgccat acgcttcttg ccttcaaagt
     1981 tgaaaggctt agtgacgaca gcaacagtca ggatacccaa atcttttgcc acttcagcga
     2041 cgactggtgc tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt
     2101 ctgcaccttc cagcgccgca cgcaatgcat cgcgatcctc atcagccgca ttgcggccaa
     2161 cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct
     2221 gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac
     2281 cttcaatgcg ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga
     2341 tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat
     2401 ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc
     2461 gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta
     2521 cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg
     2581 aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac
     2641 gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc
     2701 caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt
     2761 cctgcaactg caatatctct tcgttgacca ggttgagcag ctcggtatag cgcggctcga
     2821 tcacctctgc cagggtctga cgttgcagac tccgcggcgg acgaccacct acgctcggca
     2881 cttccacgct ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa
     2941 tcgcttcggc gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat
     3001 tgccagcata aggaattacc ttagtgtggc gcaatgcccc accggtataa acggcgatat
     3061 ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca
     3121 ataccgaata acttgatgcc agaccggcaa atatcagttg gtcaactttc agcccacaac
     3181 gttcaaccgc tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt
     3241 ttgcctgcat ccgcacgccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa
     3301 tcgcatactc ttgcgggatc acatgcagta cacgatgctc atcgcgcaca cgcaccgatt
     3361 tcgcggtatg gacgacgttt tccacatctt cttgcgtcac ttcttcttca gaaataggca
     3421 ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag
     3481 agatctgaca atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca
     3541 cggattcgag gtcgttcacc ccgcctttat ccataccacg cgacgggcag ctgcccacgc
     3601 caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg
     3661 taccaatctc cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc
     3721 ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc
     3781 cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg
     3841 ctgctgtaaa accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg
     3901 gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat
     3961 cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc
     4021 ctgcaacact tcattggcgc tgccttccgg gccatacagc attggaagca cctgcttgct
     4081 ggtgcgatct ggcggcacgc tgaaggtgtt tccttccgcg tctaccatgt gttgatcatt
     4141 ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt
     4201 tctgacgctc acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt
     4261 gacatcctgg gtcataaagg tacccggctc acccaatgct aggatcgact gccggatatc
     4321 gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg
     4381 cgcatcttcc atccagccca acacgaccca gccgctcacc aacactgtcg ttaaaacggt
     4441 cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc
     4501 ttcgctgttt cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc
     4561 gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt
     4621 ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca
     4681 gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta
     4741 atgcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct
     4801 catcagagag atacttcgcc tcataatcat agaaggttcc ggccggttga atacgtattg
     4861 acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt
     4921 caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt
     4981 ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg
     5041 gcaaacccag agcagaaatt tctgctaact gcttatcgct caggcctttt tcaaactctg
     5101 cgcgggttaa cgctacccac ggcgcgaccg gtaaaccggc accttgccat agaagtttgc
     5161 tgcgtagttt atccattgaa agcgcagatg ccatcactcc gcttccggta taaggcaagc
     5221 ccatcagctc gagcatcccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga
     5281 taaacacttt ctgaaagccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga
     5341 caggatacgc gtcaataccg ccttcacgca gtccggctaa caccgctgcg ccagaattca
     5401 gagaaacttc ccgctcagcg gaggtcccac ccaacaggac cgcgatttta tcagtcatgt
     5461 tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg ggcaattttt
     5521 ccaatattac cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc
     5581 atctcggcta cccgcgccgg atccggcacc agaatgggat caattttccc acgtccacga
     5641 attgtgcgac acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac
     5701 acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg
     5761 tcgcgcgtac gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag
     5821 cctgcgcgcg ccgctttaat ggtggcgtcc acttccgtcg ggtggtggcc gtagtcatcg
     5881 accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa
     5941 tcaaaacggc gaccagtccc ctggaagctt tcaagcgccc gcaaaatagc ctcgtcgtca
     6001 atgccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacgacct
     6061 ggcgcattca gggtgacgcg catcggctct ttgtcctggc gcagcagcgt aaagtgcccc
     6121 tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa
     6181 gtcgtggtct gacgccccac tcgcggtaac aattcgcgga tcaccggatc atcaacacac
     6241 atcaccgcac gaccgtaaaa cggcaggttg tgcagaaaat taataaaagt ctgttttaaa
     6301 ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc
     6361 accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac
     6421 cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag
     6481 gttgggtcga gccccgcttc tgcgtagatg ctggaaacca tcgcggtggt tgtcgttttg
     6541 ccgtgcgttc cggcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg
     6601 gcacgacgga tcaccggaat acgcgcttca tgagcggcga caatttccgg gttatcggca
     6661 gaaatcgcgc tggaaacaac gaccacgctg gcatcacgta cgttttccgg gcgatggttg
     6721 aaataaatcg tcgcacccag attcattaac tgctgcgtga ccggatttgg cgctaaatcg
     6781 gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca
     6841 ccaccaatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc
     6901 agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat
     6961 gcaaaaggca tcgctacaat tacgcccggg caacccggct cacttcattt gccactcgct
     7021 cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc
     7081 gcgaccaccc ggccaaggtg ttggcgacag catccacgct aagctgtggc tgctcgataa
     7141 ttttggctgc gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat
     7201 gttgaaacgg cacaaacaac gccggtagtc ctgccgcggc gatttcactc accgttaacg
     7261 caccggagcg gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa
     7321 attccgtcac tttatgctgc ggttgccccg cttcggcata cgcctgttca acggattgtt
     7381 gcgaaccttt gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct
     7441 gcggcattgt ctggttaaga atgcgtgcgc cctgagaacc acccactacc agcacacgaa
     7501 ccggaccttc acgtccagcc aaacgttgct gcggcaacgg cagcgccaac acatcggtac
     7561 gcaccgggtt acccactact tccgcattag ggaaagcacc tggaaacgcc tgcatcactt
     7621 tggtggcaat cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa
     7681 gtacaaccgg aatgcctaac gaccacgcgg ccagaccacc tggacctgac acgtagcctc
     7741 ccataccgag caccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg
     7801 cgttgaagat acgcagcggg gcagctatca gtgcttttat accttttcca cgcagaccag
     7861 agatacgaat gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt
     7921 cggcagtccc cagccagcga acttgccaac cctgagccat tagatggtgc gcaaccgcca
     7981 gtcccgggaa tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac
     8041 cactcatcgt gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat
     8101 acgcaacagc atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat
     8161 cagcggcaat gtcagacctt tggtcggtaa catccccgcc gccgcgccta cgttaaccag
     8221 cgcctggaag ctaaaccaga tgccaataga acaggcgaga aaaccggaaa aacggtggtc
     8281 aatttctaat gctttacggc caatcgacat cgcgcgaaaa gcgacgaaga ataccattaa
     8341 aagtgccagc accacaccga cataccccag ttcttcgccg ataatggcga aaataaagtc
     8401 agtgtgcgct tccggcagat actccagttt ttgtaccgag ttacctaaac cttgccccca
     8461 aagttcgccg cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg
     8521 atcttcccac gggttccaga atgcggtaac acggcggata cggtacggtt cggcgagtat
     8581 cagcaacaca accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc
     8641 tcccgccagg aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc
     8701 tggctgtgcc agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc
     8761 gcgcaggtta ttacgtactt cgtcgccttt acgcaccaga tagttggcga tatagcaaaa
     8821 cagcgacagt tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg
     8881 cgatgcccct ttaaccgagc tacccactac caggacgatc atcagcagga tgatagatcc
     8941 gagcagcatc gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat
     9001 cgccagaata aacgccaaaa tcagatagac accatcacgc ttcgcgaaga agaacggatc
     9061 gttggttaag cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc
     9121 cgcgaggccg aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc
     9181 tttttcccgc gagcccatca cccagccctt tagcgccgtg gagatccaga ccaggatact
     9241 gaatcctggc aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc
     9301 ttcgccagac gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg
     9361 gcacaggctg gggagagcag aaccatatcg cccggctgaa cacgcggagc cagcaagcgc
     9421 atcgcctgtt ccatagtttc ggtttgttct gccacttccg ggcgtagcgc cgccagctgc
     9481 gcgccgtcac gaccgaaaca atacagacgt acgttatcgc cattcaggta acgcgccagt
     9541 gggctaaagt ccgccgattt accatcgcca cccagcaaca aatgcagtgt gccgtctacg
     9601 tgcaggccat tcagcgccgc ttccgtactg ccgacgttgg tcgctttcga atcgttaatc
     9661 caacgtacgc cgttatgctc cagcacaact tcaaagcgat gcggcagacc agtgaatgtg
     9721 gttaacgctt tcaggctgct ggcacgcggt aaccctgcag catctgccag cgccagcgcc
     9781 gccagcgcat tggtgtagtt atgctgcccg gaaagtttca tctctttcac attcagcact
     9841 ttctcgcctt taacccgcag ccaggtttcg ccctgctgat gattcaggtg atagtcaccc
     9901 atgttgacgc caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca
     9961 tcatcagcat taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga
    10021 tactgttgta aaccaaacgg atagcgatcc atatgatctt cagtcacgtt cagaatggtc
    10081 gctgctaccg cctgtaagct agaggtggtt tccagctgga agctcgacag ttccagcacg
    10141 tacagttcgc actcatcatc cagcagcatc aacgcaggca ggccaatatt gccacccact
    10201 ccaacgttaa cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg
    10261 ccgttagaac cggtaatcgc cacaatcggt gcttgtgctt cgcgacaaaa cagctcgata
    10321 tcgccaacga tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca
    10381 ataccgggac tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc
    10441 gtgtggcgtt ctacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc
    10501 ataacgcgcg gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccggtgagg
    10561 cccaggccga taatgacaac atttttaccc tggtaatcag ccatgattaa cgtaccttca
    10621 gcgttgccag accaatcagg accagcatca gcgaaataat ccagaaacgc acaatgacgc
    10681 gcggttccgg ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac
    10741 gttgtccgcg cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga
    10801 acacgccccc cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta
    10861 acgcaccacc taacgccagc gacccgacat cgcccataaa gacctgcgcc ggataggtgt
    10921 taaaccacag gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc
    10981 cggcgtgtcg cagatacggt atgtgtaaat agctggcaaa gttcatgttg ccggtcgccc
    11041 acgccaccag cgcaaaacca ccggcgacaa atacggtcgg cataattgcc agaccgtcaa
    11101 gaccatcggt caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa
    11161 tgtagaacag ccccagttgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg
    11221 cgggcgtgtc tttgccggca aggtacaaag cgaaagcaac acccagagca atgaccgaca
    11281 tccagaaata tttccaacga gcgatcaacc ctttggtgtc tttacgcacc actttgcgat
    11341 aatcatcaac aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt
    11401 acggattgga cgggtaagcc cacagcagca cggagatcac aatcgctgtc aggatcataa
    11461 tcccgcccat ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca
    11521 ctacctgacc aaaggaaagt ttttgcaaat gagcaatcat gcgcgggccc atccacaatg
    11581 agatgaacag cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag gaaaagacgt
    11641 taaagccgga ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc
    11701 ctgtaaagcg cgtactacct cttccatggc agcactacgt gaacccttaa ctaaaatcgt
    11761 aattacctgt tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc
    11821 aaaatgttcg ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccacgcttaa
    11881 cacgcggtca ataccagcag cttttgccgc ctcgcccacc tgtacatggc aggcttcgct
    11941 ttcagcgccc agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc
    12001 cagtacctga acagcggcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag
    12061 caactggttt tctgccagtt ggatggggaa cagacggcca ggaacagctt tcagatttgc
    12121 cagccccgct ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc
    12181 cagcgcattc gcaatattgt gacgccccgg caacggcagc agaacatcga cgctaccggt
    12241 tggggtttgt agggtaaatt ccgtaccgtg cgaggtcaca tggatattgg tggcggtgaa
    12301 atcgctgttg gcggcattgg gtgagaaacg ccacactttg cgtgagccaa ttacgctctg
    12361 ccagttcagc cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc
    12421 gctaaagatt tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg
    12481 cgccgctgcc aggttgttga ccagcgcagc ttccgggcga gtcagactca cagtccaggc
    12541 tatttcgccc tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa
    12601 gcgcaacagc gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt
    12661 gttgccgcac tggcttaaaa tagccgccgt catctcttta acggaggttt tgccggagga
    12721 acctgtcaga gcaaccacgc gcgccggaac ttgctggcga acccatgcag ccagttcacc
    12781 aaacgccaga cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca gcggacggct
    12841 aaccagtagt gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcat gagcatcgaa
    12901 acgttcgcct ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt
    12961 tacagcatca agggtgatat ctgcaccttg cagttcaccg ttgagaatgt cggtaagttg
    13021 gctaagggtt acactaatca tgcaatcacc cccagcagac gcgccaccgt gacgcgatcg
    13081 gagtagtcca gacgctgatt gccaacaatc tggtaatctt catggccttt gcccgcgacc
    13141 agcaccacat cgttctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct
    13201 tccatcactt tggcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca
    13261 cgcggttctt cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg
    13321 gcaattgcgc ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagacg
    13381 caccacagct tgcccgcaca gtgcagccgc gccgcctgta aggctttttc cagcgcatcc
    13441 ggcgtatgcg cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa cacttccata
    13501 cgtccgcaaa ccggttgcag acgcgcggcg gttttcaaca gatcagccaa tggatagccg
    13561 agtgccaaca gtgtcgccag cgcgagcagc aggttgctga cgttaaaagc gcccatcaga
    13621 tggctttcaa tttcgccatc gccccaactt gagctaaagc gaatcgtcgc accgctgtcg
    13681 tgatagttca cgtcgatcgc ttttaaccag cgtccgtgac agttcggatt aatatgatct
    13741 tccattgata ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg
    13801 tcggcgttaa taatcgcctg accgcaatga tgctcagaat aaagcagcca tttcgcggct
    13861 tcgtagtgtt ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc
    13921 gacgccgcaa atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc
    13981 attgcgcaaa acgtcgcgcc ctgatccacc agccccgcca gctcatgctg aacatcgact
    14041 gccgaaccgg ttgtattttc tgtcgggatc actttcccca gcaggccgtt accaacggtg
    14101 cccattaccg cgctggtttc gccaagcagt tggctccact gcgccaacag ctgggtagtc
    14161 gtggttttgc cgttggtgcc cgttacgccc acgagacgta aattgtcaga gggttcatga
    14221 taaaagcggc ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt
    14281 acgccgtgca tttcacggat ttcaccatcg gtcgcctcat ctttcgcctc tgcaataatg
    14341 gcagccacac cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct
    14401 actacagcta caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc
    14461 agtgctcgcg aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct
    14521 gccacctgtc ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc
    14581 atccggctcg atgttcatgg tacgcaatac gccgcccatg atggcaccaa agaccggcgc
    14641 ggaaacggcg ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc
    14701 gaagcgcggc tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta
    14761 gcgaccgtcc ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt
    14821 aatcgccgcc ttcacgccgc cgccgcctgg tagcgccacg ctttccatca tatgcaccac
    14881 agtgcggaca atggattccg ggaagacacg ttcaccggga accggggggt caactttggt
    14941 aatcgacagt gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg
    15001 taacggtgtt accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca
    15061 ccgttgtttt tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt
    15121 tcccagtcca aaacgtgagt aagtatctac taacgctgag gacggcatcg ctaacgccag
    15181 cttggaaaca ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta
    15241 gcgtgccacg tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga
    15301 gttttcccgc accacgccac gttgcaacgc ggtcattacc accatcggtt taaccgttga
    15361 gcccggttca aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccgct
    15421 cagattgtta gggttgtatg acgggctgtt agccatcgcc agcacttcac cggtgttgac
    15481 atccaccagc acggcgctac cagattcagc cttgttaaag gccaccgcgt tgttcagttc
    15541 gcgataaacc agcgcctgca ggcgttcatc aatactcagc gccaggttgt gcgctgcctg
    15601 gctgtcagta gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg
    15661 ctcacccggc tgcccggtaa gccatttatc gaaactcttc tcaacgccct caatcccttg
    15721 actatcgacg ttagtaaagc cgatgaggtg agcagtcact tcgccggacg gatagtaacg
    15781 gcgagactct tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat
    15841 gtcagggttc acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg
    15901 ggctgaaagc tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc
    15961 gacgctgata ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg
    16021 cacgctcacc gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac
    16081 ttgctgaacg cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac
    16141 ttgtaaccac gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa
    16201 caacgcaaaa cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt
    16261 tttcgccgct gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg
    16321 tgacggatca acatgctgca tttgcagctt ttccgtggcg atcctttcca cccggctatg
    16381 gtcgccgagc gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg
    16441 ctccagcacc agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac
    16501 cacagtcacc gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg
    16561 caaaagatcg tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc
    16621 tttaactttg cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata
    16681 cgcagaactg aactacgggc acgagggttc tcagccacct cttcttcgcc cggcattaac
    16741 ttgcctagtg ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt
    16801 aaccctgccg gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg
    16861 tcttccagcg agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc
    16921 gagcttttta gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc
    16981 tggaaggtac gggtcgcggg atgtttaaac ttatctttca ccggcgttgc agcagccacg
    17041 acttccgcca gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg
    17101 cgggcaatgc gtttggcaaa acgctcttca ccataggttt tcaataccca ggcgatatcg
    17161 gcttcttctg cggtttgtag ccattcagcg gctgactgcc cacgggttgg gtccatacgc
    17221 atgtccagcg gaccatcgcg cataaaggaa aagccacgtt cagcatcatc aagttgcggt
    17281 gaagagacgc caagatcgag gagaatgccg tcgatcttgc cgataagatc gcgctcggca
    17341 acgtattcgc ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg atcatcaata
    17401 gtcttcgcaa cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg
    17461 ccaagctgcg agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag
    17521 atgccatcag gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt
    17581 ttatagtttt ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt
    17641 agccaactgc tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca
    17701 cagctcaaac ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg
    17761 ccgcagtact ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg
    17821 acctaacagt aggcgctgca cacggcgctc aaccgggttc atgctcgaca gacgcgataa
    17881 tttttgctcg ataatttccc attcaggcag ggggtaaagc agcaggcacg ggtgataaat
    17941 gtcaatggtg caaaccattt gaccggcagc gttctcaagc agctgttccc gataacgggt
    18001 aggcactgat aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc
    18061 agcctcaccc cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc
    18121 tgaggaaaag cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac
    18181 ggctaatatc agcgttgttt attttacgtc caacgtgcga agcaaattaa cgttatgaat
    18241 agctgtaaga aaaaatccct catcgtcatc actgttttaa aatgactcac tattttccac
    18301 agcaaatata aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt
    18361 taacagtcct tgttacgtct gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag
    18421 aaaagtgttt gttaaatcgg caattcatcg ccgcatgtaa caaaataatg caaaaacgcg
    18481 ctgtcgatat cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt
    18541 aaaagcattt atcaataata gtcaggaata gtcttattta ctttaagcat attgatgtcc
    18601 agtcccgtac tctacgcgcc agagggaaat tcacctggcg cgtatttttg ttcgcggctt
    18661 agctacggct gagcacgccg cggcgataga gattacgttt aatgcgcgtt aaaccaggtt
    18721 ttggcttacg cggttcgtcc aggcttgcca ggacaatctc cagcacacgc tctgcgacat
    18781 cgcggtgacg ttgagccact gccagcaccg gacactgtaa gaagtcgagc agttcgttat
    18841 cgccaaaggt ggcaattgcc aggtcagaag gcagtttgcc gtcgcgacgc agcgtgacat
    18901 ccatcactcc ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg
    18961 tttccagcca tttttcgaat aactgggcag ccgcctcccg ctcatagctg ttggcataca
    19021 ggaaatgcac ttcgcgcgga tcatctttcc aggcagtacg gaaaccttgt tcacgcagga
    19081 agctgacaga aagctccggt agcgcaccaa gataaagcac cgtctcggcg ggaaacttac
    19141 gtaactcttc cgccagcatt tcggcatcat cctgatcggc accaaccacg ctggtgaagt
    19201 gttcacgatc gagggcgcgg tccagcgcga caatcgggaa cgggtcgtta gcccagcgtt
    19261 gataaaaagg atgctcagga ggcaacgacg tcgaaacaat aatggcatca acctgacgct
    19321 gtaaaaggtg ctcaatgcac cgcatttcgt tgtctggctg atcttctgag caggcaatca
    19381 gcagttgata accccgttgc cgcgcctggc gttcaagata gttagcgatg cgggtatagc
    19441 tggtgttctc cagatcgggg atcacaagac caatagaacg tgtgcgtcca gcacgaagcc
    19501 cagctgccac ggcgttcggg tggtaattgt gctcacgcac cacagccatg actttttcaa
    19561 cggttttgtc gctcacacgg tattgcttcg ctttgccgtt aataacatag cttgcagtgg
    19621 tccgcgacac tcccgccagc cgagcgattt catccagttt cacaattgcc ccttgcgtaa
    19681 aatgtaaaaa ccatagccat tgtactggta tgggttaaat tcattaacat ctaagcgcag
    19741 aaaattaact gcggcaactg cttttattcc aggttcccac tgatttcgca aaaaaagccc
    19801 aacgtgacat gttgggctgt aaattgcgca ttgagatcat tctcaacgca ttattttatc
    19861 gccgcgcgaa agtccgacca caccagagcg agcaacctcc acaattttcg ccacatcgcg
    19921 aatcgatgct aaaaatgcat caagcttacc gctggtgcct gctaattgaa cggtataaag
    19981 cgagggtgtg acatcgataa tttgcccacg gaatatttcc gtattacgtt tcacttcgtc
    20041 acgcccgtaa ccgctggcct gaattttcac cagcatgatt tcccgctcaa catgcgcgcc
    20101 ctgccccaac tcactcacgc gcaagacatc gaccagcttg tgtaattgct tttcgatctg
    20161 ctcaagtact ttttcatcgc ccacggtctg gatggtcata cgcgataatg tcggatcgtc
    20221 ggttggcgca acggtcaggc tttcaatgtt gtagccacgc tgggaaaaaa ggccaatcac
    20281 gcgggataac gcgcctgatt cattttcgag taagactgat aatatccggc gcataatcag
    20341 gttctctccg ttttgcttaa ccacatttca tccattccgc ccccgcgaat ctgcatcggg
    20401 tagacgtgct cgctgccatc gacggtaaca tcaacaaaca ccaggcgatt attgcgcacc
    20461 tgttccagcg cctcgctaag tttgctttcc agctcatgcg gatgagaaat ctggatcccg
    20521 acatgcccat aggcttccgc cagacggacg aaatcgggta gcgattgcat ataagattgt
    20581 gaatgacggc cggaatagat catgtcctgc cactgcttca ccatccccag atagcggtta
    20641 ttgagattca ccaccagtac gggcaactcg tattgcaacg cggtagacag ttcctggatg
    20701 ttcatctgaa tactgccgtc gccagtgacg caaaccacgg tttcttctgg caacgccatt
    20761 ttgacgccca gtgccgcagg taaaccaaaa cccatcgtgc cgaggccacc ggaattgatc
    20821 cagcgacgcg gtttgtcgaa tggataataa agtgcagcaa acatctggtg ctgcccgaca
    20881 tcggacgtca cgtaagcgtc tcccttcgtc aaccgccaaa gagtctcgat caccgcctgc
    20941 ggtttaatct tttcactgtg agtgtcatat ttcaggcact gacgagcgcg ccactgttca
    21001 atttgctgcc accagtcgcg gatctcatcc agtggttgat gggcggattc ttgcgacaag
    21061 agttcaagca tttgttcgag gacctggcga gcatccccca caatcgggat atccgcagtc
    21121 acggttttag aaatggaagt aggatcaata tcgatatgca gaacagtggc atttgggcag
    21181 tactttgcca gattgttcgt cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca
    21241 tccgcgttat gcatcgtcat attggcttcg taggtaccgt gcattcccag catgcccagt
    21301 gcctgacgat gcgttgccgg aaacgccccc agccccatca atgagcaaac aacgggcaga
    21361 ttcaacgcct ccaccgtttc tttcaactgc tgatggcagc ccgccgtgat tgccccaccg
    21421 cctacgtaga caaccggttt ttttgccgct accagcgttt gcagagcacg cttaatttgc
    21481 cctttatgtc cggtagtagt gggattgtaa gaacgcatac tgaccgactc cggccagaca
    21541 tagggtaatt tgttcgccgg attaagaata tctttcggta aatcaacgac tactggtcct
    21601 gggcgaccac ttgccgccag ccagaaagcc tttttcagca cctgcggaat gtcttccgtt
    21661 tgcttaacca gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc
    21721 tgaaaggcat cgtaacctat caacgaggtc gctacctgcc cggaaaggac aactaatgga
    21781 atggaatcca tataagcggt ggcgatgcca gtaatcgcat tggtcgcccc tggacccgac
    21841 gttaccagca cgacgccgac ttccccggtc gcgcgcgcca ggccatcggc catatgcacc
    21901 gccgcctgct catgacgaac taatacatga tcaataccac ccacggtatg caatgcatca
    21961 taaatatcaa ggactgcgcc tccgggataa ccgaatactt gtttaacgcc ctgatcgata
    22021 agcgatcgga cgaccatctc ggctccagac aacatctcca tggcctgcct cactgtttga
    22081 cggaaaaaaa tgtgtaaaac acattaaccg tccgatcttg cttaagcaat tggcagccag
    22141 agacataaac acggggtaat aagaataaac aggaggaaag gtgaaaagaa acgataaaat
    22201 cctccattac agaggattaa attgataata aatcggctga atcccacaac ttacagcaat
    22261 ttctctattt ttttaattaa tcctaaaata gagagcccac acattcagaa taaaattcta
    22321 cattcaataa aaaatgcaga ataaaccaga cattcatgtc tgacctattc tgcaatcagt
    22381 tagcgtttgc aaattgagac taattgctct tccatccact gatggccttt atcgcgcccg
    22441 gcagcttcat gccaggagag ataacaggtt ctgctgtttt gttttaacgg cagcggtaat
    22501 acctgtaatt ctaaggattc agcgaactct tcagccagcc aacgcggcgc aatagcgacc
    22561 aaatgcgttt gcgacaccac gctaagtacg ctcatcattg ccatgccctg atacgcgata
    22621 ctggctttct tatctaccgt gtcataccaa ggttgactaa atgacgcgaa acgatcgagc
    22681 gaaaccgccg catgttgttc gttataaaca tcatgtttca gtaacgggcc cttaattgtt
    22741 ggatgatttt tgctggctac cagcaccatt tcatctttaa ataatggtac gctggtaaat
    22801 tcaggacgat ggaagtcttc ataactaatc acaaactccg tttcctgata acgcagctga
    22861 tgttcagtgt tctgatttaa tgaagacttg aacataacat gtatatttgg cgcaatctgc
    22921 tcaatgtgat tataaatctg cgaggtcaga atgctgtcta acgggctgca aacacaaaga
    22981 tgaaatacac gttcactgct cgcgggttca aaacctgaac caggcaattc attttgtact
    23041 agttgcaatg cctgacgaac tgaaccaaaa agttgaaatg cgcgagcagt cggttgaata
    23101 ccacggccat aacgaacaaa aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg
    23161 ttactgaccg caggttgcga cattcccaga acatgagcgg cacgagtaat gttttgctcc
    23221 tgcatcacgg catcgaaaac ggttaataag ttgagatcga ccatgcgtag ctgtggcttg
    23281 cttaactccg ccgtctctgg atgatctgtt tgtacctctg gcatacttaa ctccactgtc
    23341 acacttaact ccctttccct tattggaatg cagagaaata atcctcgaga atatgattta
    23401 tcatgcatat aaaataagaa aaaggaaaat gagtaaaatt aggaaaacat aaagatacgg
    23461 gttctgataa aaacagaatc cattgccgtg gaatgagtca tttaccacag cataataatc
    23521 cataatgttt ttgttgcgaa aacaatctaa tcataactac cgcgaatact caatcatcta
    23581 caaaatggat taaatgtgct tatttaataa ttaatttata tatttaatgc attaattctt
    23641 aacattaatt gatcaataat attcaccaaa tcaatatcaa aaaaaaaaaa atcgcaaaac
    23701 atataattca ataaaaatca tcaggatagg ttttgcaacg cgtgcacttt gtcccctttt
    23761 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
    23821 tagttaagca gaaattaata tcgcttactt taaccaccgc agcacaatta gctaatttta
    23881 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
    23941 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
    24001 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acaagtgagc
    24061 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
    24121 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
    24181 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
    24241 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
    24301 tcgggttccc cgtttcttcg ccgggtgatt ttgaatcagt gcaaaccatc gctcgccagg
    24361 ttaaaaacag ccgcgtatgc gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
    24421 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgcc acttcgccaa
    24481 tgcacatcgc caccaagctg cgcagcacgc tggacgaggt gatcgaacgc gctatctata
    24541 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
    24601 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
    24661 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
    24721 gcggcctgta tgaacgcgtg cctaacatcg acaaagccat tatctccgta catacccacg
    24781 acgatttggg cctggcggtc ggaaactccc tggcggcggt acatgccggt gcacgccagg
    24841 tggaaggcgc aatgaacggg atcggcgagc gtgccggaaa ctgttccctg gaagaagtca
    24901 tcatggcgat caaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
    24961 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
    25021 aagccattgt tggcagcggc gcattcgcac actcctccgg tatccaccag gatggcgtgc
    25081 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
    25141 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
    25201 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
    25261 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
    25321 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
    25381 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
    25441 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgaa tataacgtcg
    25501 aactggtgaa atacagcctg accgccaaag gccacggtaa agatgcgctg ggtcaggtgg
    25561 atatcgtcgc taactacaac ggtcgccgct tccacggcgt cggcctggct accgatattg
    25621 tcgagtcatc tgccaaagcc atggtgcacg ttctgaacaa tatctggcgt gccgcagaag
    25681 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
    25741 gtcgaagaat taccatattg ccgtattgcc gggggacggt attggtccgg aagtgatgac
    25801 ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
    25861 ccattacgat gtaggcggcg cagccattga taaccacggg cagccgctgc cgcctgcgac
    25921 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
    25981 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgcctc tgcgtaagca
    26041 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
    26101 tccgctgcgt gcagacattg ccgcaaacgg cttcgacatc ctgtgtgtgc gcgaactgac
    26161 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
    26221 ctttgatacc gaggtgtatc accgttttga gatcgaacgt atcgcccgca tcgcgtttga
    26281 atctgctcgc aagcgtcgcc acaaagtgac gtcgatcgat aaagccaacg tgctgcaatc
    26341 ctctatttta tggcgggaga tcgttaacga gatcgccacg gaatacccgg atgtcgaact
    26401 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
    26461 cgtcctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
    26521 tggctcgatg gggatgttgc cttccgccag cctgaacgag caaggttttg gtctgtatga
    26581 accggcaggc ggctcagcac cagatatcgc aggcaaaaac atcgccaacc cgattgcgca
    26641 aattctgtcg ctggcactgc tgctgcgcta cagcctggat gccgatgatg cggcttgcgc
    26701 cattgaacgc gccattaacc gcgcattaga agaaggcatt cgcaccgggg atttagcccg
    26761 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
    26821 aggggtgtaa tcatggctaa aacgttatac gaaaaattgt tcgacgctca cgttgtgtac
    26881 gaagccgaaa acgaaacccc actgttatat atcgaccgcc acctggtgca tgaagtgacc
    26941 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
    27001 accttcgcta ccatggatca caacgtctct acccagacca aagacattaa tgcctgcggt
    27061 gaaatggcgc gtatccagat gcaggaactg atcaaaaact gcaaagaatt tggcgtcgaa
    27121 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
    27181 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
    27241 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacgcaa
    27301 accctgaaac agggccgcgc aaaaaccatg aaaattgaag tccagggcaa agccgcgccg
    27361 ggcattaccg caaaagatat cgtgctggca attatcggta aaaccggtag cgcaggcggc
    27421 accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg atttaagcat ggaaggtcgt
    27481 atgaccctgt gcaatatggc aatcgaaatg ggcgcaaaag ccggtctggt tgcaccggac
    27541 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
    27601 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
    27661 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
    27721 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgc
    27781 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
    27841 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
    27901 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
    27961 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatctttatt
    28021 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
    28081 cgtctgaatc cgggcgaacg ttgtgcctcc accagcaacc gtaactttga aggccgccag
    28141 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
    28201 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
    28261 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
    28321 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
    28381 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
    28441 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
    28501 tgagcacgcg ccctgggcaa tgaccgacta cggttttaaa gtggtgattg cgccgagttt
    28561 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    28621 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    28681 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    28741 cttccgccgc cactgcatga tgaacggtct ggacagtatt gggcttacct tgcagcacga
    28801 cgacgccatt gccgcttatg aagcaaaaca acctgcgttt atgaattaat ccccttgccc
    28861 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    28921 ttaaaaataa tgcgacaaca gaaataaccg caattaccca gtagacagca aagtgccccc
    28981 aactttgtgc aattgctccc tgaataacgc cagccagaat taccccggta gaaatactgt
    29041 tagtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    29101 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    29161 ccatacggct attaaagaaa atcaatccgg tgtaaaacag tactcctgcc gccactgcta
    29221 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    29281 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    29341 actcgctact gatccacaac ggcatatcaa taatgtacat ggtgttgcag gtccacatta
    29401 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagccacct tgcattgata
    29461 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
    29521 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat caccgtaaag ccgtaattca
    29581 acgccagcat aaaggccaac ggtggaccga taacccatgc cagagaaagc tgcgcacgca
    29641 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcatattcc cgcgccagag
    29701 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    29761 gcgtcagata atgacgatta aatgcaaaca atagcgcatt gccgatagcc atcaaacagc
    29821 aaaatataat cagttttcgc cgatcgccct gattgtcaga acgttttgcc aaccagaggc
    29881 ttacgccgat cccagcaata gcattcaccg tataaaagag gccgatccag aaaggttgcg
    29941 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    30001 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    30061 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    30121 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    30181 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    30241 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    30301 ctgacgcatg gggcaccccc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    30361 ataaaattgc tgtcttttgc acaggagttc cccttttatg ccatctgctc gtctgcaaca
    30421 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    30481 actggcagcg ttattgagct gctcgcgtcg tcatatgcgc accctgctca acaccatgca
    30541 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgt ggtaaacgct cgcgtctgac
    30601 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    30661 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    30721 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    30781 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    30841 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    30901 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    30961 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    31021 caatacgctg ccgctctatt cgcatattgc tgacattgtg tcgccgacgc cctggacgct
    31081 ggatatccat ctcacgcaac cggaccgctg gttaccgtta ttgctggggc aagttccggc
    31141 gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt gccagccatc ccatcggcac
    31201 cggtccgtat gcggtgatcc gcaacagcac caatcaactg aaaattcagg cattcgatga
    31261 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    31321 cgacgagcca gctggaggtc tgatgctaaa aggtccacag ggtgaggaaa aagagattga
    31381 aagccgcctg gaggaaggtt gctactattt actattcgac agccgcaccc atcgcggggc
    31441 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    31501 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    31561 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca tcctgacctt
    31621 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    31681 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    31741 cgagagcgat atctggctta acagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    31801 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    31861 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    31921 cagcaaagca atggtgccgc ttatccacca ctggctgatc attcaggggc aacgcagtat
    31981 gcgcggcctg cgcatgaaca ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    32041 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    32101 gtgccacgca aacgcgtgtg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    32161 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    32221 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    32281 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    32341 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    32401 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    32461 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    32521 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    32581 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    32641 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    32701 cgccggggcg gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac accccgcagg
    32761 cctggcagaa actggcgaag aaaactgtca ccgtcaccaa aggctggagc gaagcctacg
    32821 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    32881 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    32941 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    33001 ttctccagtt tatggtttct ccggctttcc agaatgcgat cccaaccggc aactggatgt
    33061 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccagcaacca
    33121 cgctggagtt cacgccagcc gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    33181 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    33241 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    33301 ctgggtggca atctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    33361 ggcgtttctc tcggcgctgc tctctgtcgt acccgcgata ttcctcgccc gcgcactcta
    33421 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    33481 cccggtgctg gttgctgttt tcggcattct tagcgtctat ggtcgccagg gctggctggc
    33541 aacactctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggtat
    33601 tttgctggcg cacgtatttt ttaatctacc gatggcgagc cgcttattac tccaggcact
    33661 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    33721 ttttttccgc ttcgtcgaat ggccgtggtt acggcgacaa atcccgccgg ttgctgcact
    33781 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    33841 ggcaaccact atcgagctgg caatctatca ggcgctgagt tacgactacg atcctgcccg
    33901 cgcggcgatg ctggcgctga tccagatggt gtgttgcctc gggttggtgc tgctgagtca
    33961 gcgattgagt aaggccattg cgccaggcac cacgctgctg caaggctggc gcgacccgga
    34021 cgatcgtctg catagccgca tttgcgacac ggtgttaatt gtgctggcgc tgctgctgtt
    34081 gctgccaccg ttgctggcgg tgatcgtcga tgggctaaat cgccagttgc cggaagtgct
    34141 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    34201 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    34261 gcaaaaaatg ctggccggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    34321 ggggattgtg ctggcaacgg gcttcttttt gctgctcaac aacaccattg gcctgccaca
    34381 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    34441 agtgctggaa aacccgatgc gcgatatcac cgctcgctac aacatgttat gtcagtcgct
    34501 gggcattgaa ggctggtcgc gcttaaaagt ggtggagctg cgcgccctga aacgcccgct
    34561 ggcgcaggcg ctggcttttg cttgtgtgct gtcgattggt gattttggcg tggtggcgtt
    34621 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    34681 tcgcagccag gacggtgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    34741 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    34801 atcaccattt gccgatgcgt tttagcttaa cggtagaatg cggcgagcag gtggcgatcc
    34861 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gatcgccggt tttctgacgc
    34921 cagccagcgg ttcgctgact atcgatggcg ttgatcacac cacgacgccg ccgtcacgcc
    34981 gtccggtgtc gatgctgttt caggaaaaca acctgttcag tcacctgacg gtcgcgcaga
    35041 acatcgggct ggggctaaat ccgggattga aactgaacgc ggcacagcag gagaaaatgc
    35101 acgctatcgc ccgccagatg gggattgata atttaatggc gcggttacct ggcgagcttt
    35161 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    35221 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    35281 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    35341 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    35401 agggtaagac caatgagttg ttgagcggta aggcgagtgc ttcggcacta ttggggatta
    35461 cgggttagta tgcactgtcg gatgcggcgt gaacgcctta tccgacctac gggtagtggg
    35521 ttttgtaggc ctgataagac gcggcaagcg tcgcatcagg caacccgcac aagactatct
    35581 cctaaccccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    35641 ttaacaccac cagcgccacc acgccgatgg cagaaattaa cggcgtcagc cacaacaaac
    35701 gaccacggga caaataatga ctcaaacggt cagtcgcttt accactgcgc cataaccgcc
    35761 agcacagcca gccaccaacc cacaaaaaca ccgctgttgc cagcagcaac catttaaact
    35821 cgccactctg cattccggct ggaatttcga tcgccgcacc cgccagaatc cccggcagga
    35881 agtaaaacgg cggccacagc aggcagccga taatattcgg cgtaataaat tttgccaccg
    35941 ggagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaaacggc
    36001 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcaagtagtg
    36061 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    36121 agaaggagat ccagtcgccc agcaggcaac cgacaatccc cgccatccac gcgtgccaga
    36181 agcttaactc gccgctgcca atcagtgccc ccagtcctgc cataagtacc gtacctggca
    36241 gaatcagacc gaccagcgcc agcgactcca gaaaagccac cagcacaaca gccatcagag
    36301 aatacgcggt cgattgagta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    36361 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    36421 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggct
    36481 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    36541 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    36601 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    36661 tggcggaaaa gatgtgacag acgcgacggt gacaagcaaa catgctgtgc gacgctggcg
    36721 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
    36781 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    36841 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    36901 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaaaccc
    36961 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    37021 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
    37081 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
    37141 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    37201 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    37261 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    37321 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    37381 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    37441 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    37501 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    37561 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    37621 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    37681 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    37741 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    37801 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    37861 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    37921 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    37981 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    38041 tacccgtttg tgccacgcgc cgggcaatgt tgactactcc cgctatattg gcggtattta
    38101 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc
    38161 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    38221 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    38281 cgaaagctgg ggcggcctgc cgccagccag tttctttgac gaactggacc cgatcctcaa
    38341 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    38401 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    38461 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    38521 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgggcg
    38581 ggcagtgaaa ggtatttgcg gtcaggttga tggcagcgtg gtacctggat ttatcggtct
    38641 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    38701 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    38761 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    38821 gccggtggtg ctcgactggt ttaacggccg ccgcacaccg aacgctaacc aacgcctgaa
    38881 aggggtgatt accgatctga acctcgctac cgacgctccg ctgctgttcg gcggtttgat
    38941 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    39001 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    39061 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    39121 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    39181 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    39241 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    39301 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    39361 tgacgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    39421 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    39481 aagcgaaact gccctgcaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    39541 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    39601 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    39661 tgcaattcca cacccagttt aacgcggcgc tgccgtggga cagtatcgat atggacttta
    39721 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    39781 gtcagcaaca tgccgtggtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    39841 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgtt
    39901 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    39961 agttcggatt ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg gtgaactcca
    40021 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    40081 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggca cgtattgagc
    40141 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    40201 atttgcacgg tctgaaacag cttccaggtc tggccgtaca gcgtctgatg cagcagggtt
    40261 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    40321 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    40381 aaggcaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg atttccgtag
    40441 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    40501 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    40561 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    40621 aactgccggt ggcgaatgcg ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    40681 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcactg aacctcaacg
    40741 atatgcgtca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgataccc
    40801 gcctgcccgc gtttaaagac gcgctgcgct ggaacgaagt gtattacggg tttcgccgct
    40861 aagtagccgc atcaggtatg taacgcctga tgcgatgctg gcgcgtctta tcaggcctac
    40921 acgttgcgat tttgtaggcc ggataagcaa accgtatccg gcattcaacg cctgatgcga
    40981 cgctggcgcg tcttatcagg cctacacgct gcgattttgt aggccggata agcaaagcgc
    41041 atccggcacg aaggagtcaa catgttagaa gatctcaaac gccaggtatt agaggccaac
    41101 ctggcgctgc caaaacataa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat
    41161 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcat catgaccgct
    41221 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtgc gaaaaagccc
    41281 tcctccgata cgccaactca ccgactgctc tatcaggcat tcccgtccat tggcggcatt
    41341 gtgcacacac actcgcgcca cgccactatc tgggcgcagg cgggccagtc gattccagca
    41401 accggcacca cccacgccga ctatttctac ggcaccattc cctgtacccg aaaaatgacc
    41461 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt cgaaaccttc
    41521 gaaaaacagg gtatcgatgc agcgcaaatg cctggcgtgc tggtccattc tcacggccca
    41581 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    41641 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    41701 ctgctggata aacactatct gcgtaagcat ggcgcgaagg cgtattacgg gcagtaatga
    41761 ctgtataaaa ccacagccag tcaaacgaaa ccaggctata ctcaagcctg gttttttatt
    41821 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    41881 aagggaccga agtttctttc tggctggcga cggacaacgg gccgttgcag gtcacgcttg
    41941 caccgcaaga gtccgtggcg tttattcccg ccgatcaggt tccccgcgct cagcatattt
    42001 tgcggggtga acaaggcttt cgcctgacgc cgctggcgtt aaaggatttt caccgccagc
    42061 cggtgtatgg cctttactgt cgcgcccatc gccagttaat gaattacgaa aagcgcctgc
    42121 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    42181 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc gctatcgtta
    42241 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctattgata
    42301 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggttgc gggcagcgca
    42361 tcgtttatat gctggggcca gagaatggcg acgcctccgc gctcgatttt gaactggaat
    42421 acgtcgccag tcgcccgcag ctactggaaa aacttaacgc ctggtttgcc aactacgatc
    42481 ctgatgtgat catcggttgg aacgtggtgc agtttgatct gcgaatgctg caaaaacatg
    42541 ccgagcgtta tcgtattccg ctgcggctgg ggcgcgataa cagcgagctg gagtggcgcg
    42601 agcacggctt taaaaacggt gtcttttttg ctcaggctaa aggtcggcta attatcgacg
    42661 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    42721 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    42781 ttgaccgccg tttcgccgaa gataaacctg cgctggcgac ttataacctg aaagattgcg
    42841 agctggtgac gcagattttc cataaaactg aaatcatgcc gtttttgctc gaacgagcaa
    42901 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    42961 attttccccg tatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccac
    43021 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    43081 tgctggacta caaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    43141 ggctggtgga aggcatagcg cagcctgatc cagagcacag caccgaaggt tttctcgatg
    43201 cctggttctc gcgggagaaa cattgcctgc cggagattgt gactaatatc tggcacgggc
    43261 gcgatgaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    43321 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    43381 cgtcgatcac catgcgtggt catcagatca tgcggcaaac caaagcgttg attgaagcac
    43441 agggctacga cgttatctac ggcgataccg actcaacgtt tgtctggctg aaaggcgcac
    43501 attcggaaga agaagcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    43561 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaaccc
    43621 atttctgccg ttttctgatg ccaaccattc gcggagccga taccggcagt aaaaagcgtt
    43681 atgccggact gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    43741 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctatac ctgcgtatct
    43801 tccgtaacga gccatatcag gaatacatcc gcgaaaccat cgacaaactg atggcgggtg
    43861 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    43921 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaac caaaagcgag
    43981 gtcgcccctt gcaataccag aaccgcggca ccattaagta cgtatggacc accaacggcc
    44041 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    44101 tacaacccgt ggcggaggga atacttcctt ttattgagga taattttgct acacttatga
    44161 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    44221 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    44281 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    44341 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    44401 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    44461 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    44521 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    44581 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    44641 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    44701 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaaat attccagcga
    44761 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    44821 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    44881 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    44941 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    45001 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    45061 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    45121 tgcccgtcgt agcaaacagc gcctagaaca tctctgtgaa gccgaatggg acctgctggt
    45181 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    45241 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    45301 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    45361 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    45421 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    45481 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    45541 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    45601 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    45661 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    45721 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    45781 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    45841 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    45901 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    45961 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    46021 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    46081 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    46141 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    46201 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    46261 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    46321 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    46381 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    46441 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    46501 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    46561 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    46621 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    46681 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    46741 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    46801 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    46861 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    46921 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    46981 cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    47041 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    47101 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    47161 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    47221 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    47281 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    47341 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    47401 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    47461 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    47521 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    47581 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    47641 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    47701 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    47761 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    47821 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    47881 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    47941 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    48001 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    48061 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    48121 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    48181 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    48241 gcatcttcca gcgttgggcc acgctgcgct tgctgccagt taccaccgcc agtttgctgc
    48301 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactga
    48361 gcgcgggaga tccctaattc ttctgcaatg acatacagca ccgcccgttc attcgggtgc
    48421 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    48481 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattgtct
    48541 gattttccca cccggaacgc attttgcgcc gcagtacggg aagcgccatg aagattcatt
    48601 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    48661 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    48721 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    48781 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    48841 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    48901 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    48961 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    49021 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    49081 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    49141 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    49201 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    49261 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    49321 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    49381 tcaaaaagag gcaccaggac aaccggagcc ggtacgaacc gttgatgcgc tcggtaatgt
    49441 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    49501 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gccgcggtaa
    49561 agcggacctg atgaaacaac gtggtgaaaa ccgctatacc attctggata acggtagctt
    49621 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    49681 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    49741 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    49801 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    49861 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    49921 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    49981 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    50041 ttggttattc tactggaacc actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    50101 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    50161 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    50221 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    50281 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    50341 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    50401 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    50461 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    50521 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    50581 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    50641 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    50701 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    50761 ccgtattgcc tccgctaacc aggtgacgac gggtgtcaca tctcgcatat atgatgatgc
    50821 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    50881 tggcgatgac aacataacat gggagaatga cgacaaaacg ggttcactgg tgtgggcagg
    50941 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    51001 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    51061 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    51121 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    51181 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    51241 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    51301 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    51361 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    51421 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    51481 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    51541 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    51601 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    51661 cggtaaaact gaacgctgct caggcaaggc agcaacttcc tgatgacgcg acgctgcgcc
    51721 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    51781 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    51841 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    51901 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    51961 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    52021 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    52081 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    52141 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    52201 gcggccagat gggctggggc cgtattcagg agttgcccgg gatcttcgcc caggcattaa
    52261 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatatac
    52321 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    52381 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggctcgt gtgaaactgg
    52441 aacagattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    52501 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    52561 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    52621 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    52681 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    52741 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    52801 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    52861 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    52921 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    52981 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    53041 gctacgtgaa tctgtcactg cggggcagtt agcggttgaa aatgggcatt acgtggtgga
    53101 gacgctggcg cgagcgtgcg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    53161 ccccgtgcat aaaggcgtca ttaacgacgc aggcattccg tttaccggtc ataccgagtt
    53221 tttcgaagag cgttcgcggg cgaaaaaggt ggtgatgatg ctggcgaccg aagaacttcg
    53281 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagacgcta tcacccctgc
    53341 acagttgcac gaagtgattg ctattttgca tcacgattta cggaccaaat ttggtattgc
    53401 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    53461 tacggaagag atagacacca ttattccggt gctcaatgag ctgcgggcgc aggggatgaa
    53521 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    53581 tgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    53641 gcgcggtgtg aacattacgc tgggcctacc ctttattcgc acatcagtgg accacggcac
    53701 cgcgcttgaa ctggcgggac gtggcaaagc cgatgtcggc agttttatta cggcgcttaa
    53761 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    53821 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagtattgtg
    53881 tctgccatta acccgcaaaa gggccaggcg atggtcgaaa tcggccccgg tctggcggca
    53941 ttgaccgaac cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    54001 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    54061 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggccagcc gctgcgtgtt
    54121 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    54181 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    54241 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaatgtg
    54301 atcccggtac tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    54361 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgtgtgttg
    54421 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    54481 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    54541 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    54601 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    54661 ttgaggctca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    54721 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    54781 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    54841 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    54901 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    54961 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    55021 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    55081 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    55141 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    55201 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    55261 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    55321 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    55381 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    55441 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    55501 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    55561 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    55621 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    55681 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    55741 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    55801 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataagaa
    55861 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    55921 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    55981 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    56041 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttgccagg aactgctcat
    56101 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    56161 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    56221 tatttttgcg tcctggcaac ggacgaccga ttgattccca ggtatggcgg cccataatca
    56281 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    56341 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    56401 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    56461 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    56521 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    56581 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    56641 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    56701 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    56761 atcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    56821 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    56881 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    56941 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt
    57001 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    57061 cgctttcgcc gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    57121 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gtaccaccat
    57181 tttcaccccg ctggagagca gtaaacgtcc ggtaatctgc ccaaaacgac cgaatccggc
    57241 gataatcacg cgcggctgtt cttcgtcgat ctcatcggct tcacgtgctt cctcagtaga
    57301 agattgctca aggcgattga ggatcaccag cagaatcggc gttgctgcca tcgacagcgc
    57361 caccgccagg gtcagcgatt tcgcccactc cggctccagc acattcgcca tctgcgccgc
    57421 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    57481 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    57541 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    57601 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa agggttcgat
    57661 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    57721 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    57781 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    57841 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    57901 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    57961 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    58021 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    58081 gagcgccagc gtcatgccga tcaattccgc gacctgccag cgcagcccaa gtaacatgca
    58141 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    58201 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    58261 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    58321 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    58381 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    58441 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    58501 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    58561 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    58621 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    58681 gtttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    58741 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    58801 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    58861 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    58921 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    58981 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    59041 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    59101 gcggatcgtc atattgctgg tctgcgccag tgacatccct cgagtctccg gggcaaacgc
    59161 tacggaaatc aacaagccaa acagcgagat acccgccccc attagcatcg tgttactgat
    59221 accgtaatta ttgataaaga tcggtagtgc ccaggtcgaa acaatggtgc caatacgact
    59281 taaagacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    59341 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    59401 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    59461 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttagcc acagcatcgg
    59521 cggaatacag ccgagcataa agaacaggct aatcaccacg tttcccagcg ccgcgttttt
    59581 gccaaccccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    59641 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    59701 attaaacaga tcgcgaaaac gggtttgctg cggctgctct tcatcgaaag ccaccggttc
    59761 gccaaacagt ttgatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    59821 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    59881 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    59941 ggcacaggtc gcgccgacat accacatcgc ggcaataaag ctgatggaaa acgcccgctg
    60001 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    60061 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    60121 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtccgacttt
    60181 atcggagata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    60241 cagcaagcca atccagtcag cgtccagttt cagcgccggg gtcagttgct ccagcgccac
    60301 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    60361 gcggcggtga atagaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    60421 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    60481 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    60541 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    60601 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagcgcct
    60661 gtttatcagc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    60721 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    60781 gccatccttg attagattga tgaagcccac tttcttaccg tggcggagga ttttcttgcg
    60841 catcagttct ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    60901 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaac gccggtagtt tctgatacat
    60961 acgcatatcg cgcagcggac cactctcaag atgctgacga tattccgcca gtttttgctt
    61021 actgaaatcg tcgattttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    61081 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    61141 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    61201 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg caactgccgg
    61261 atgctgtttg aaatcttcca gcatttgcgg caccgatttt ttcgcgtcat gcagatgatg
    61321 cagaccacaa accagcccca gcgacagggt gttttcattg gtataaagaa aaccgccgcc
    61381 catcaggcca tcggtgggtg atcccgcaaa caggcaagcc gccccctgat taccctgcaa
    61441 ctgaaaacgg tcttcaataa ccgacttcgg taactcgatc agttccttca cgccaaccgc
    61501 cacatccgtc ggtttgacgc gttttgccat ccccaatttt tcggcaagga tggagttcac
    61561 cccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    61621 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgctcc
    61681 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctacgcaaaa cggagtaaga
    61741 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    61801 cgtcataaac gcgagttttt catgggtgat caggcgttct acgggggcgg agtcggcgaa
    61861 accaggaata atgtgttcca gactgtgggc atagagacgc ccgccggtga cgttcttggc
    61921 acctgcggaa ttgccacgct cgataactaa cacttgcgca ccttcgcgga cgagcaccag
    61981 tgcggcaacc gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga
    62041 cataagagct ccctgtcagc ggcagggccg taatgcagcc ctgccagagt ggatcaacgc
    62101 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt cgccaacaat gccgtaatcc
    62161 gcgtactgga agatcggcgc atttttatct ttgttgatgg cgaaaatggt ttgcgatgca
    62221 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    62281 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    62341 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    62401 gcaatgttct ctttgctgcc aatgccgcga ccgacgctga ccaccagacg ggctttgtcg
    62461 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    62521 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    62581 aacgtgccgc tgctgatggt cagaaccgca tacggcgtgg caatgcgttc ttcgccaatc
    62641 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    62701 ctggcatcat tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    62761 ccgcgacggg tgtttggcaa cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    62821 gccatgacac tggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    62881 acatgattag cgccgagctg gattgcctgc gcgccgtcgg catcattgag gacaaaggtg
    62941 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    63001 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    63061 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    63121 gctgacgctc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    63181 taaaaccgat atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg
    63241 ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca
    63301 gcggaatgct taaggtttct gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    63361 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccta
    63421 ccagcaaacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    63481 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    63541 gctcaaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt
    63601 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggctgtc acctgcgcct
    63661 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta
    63721 ttttggcatc ggctttactg aagtctaacg aaccatcagc attattgacc gcaatatcct
    63781 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    63841 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    63901 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    63961 gttaagttcc tcaccaatat tgaaaacacg gcgtagcaaa aagaaatttt caatattgtt
    64021 ttatggatca ccaatattga aagtcagatg ttattttttt actcacaaca gagcataaca
    64081 aactgattat taatcaaaat taacgaaaaa acgccctcta ttcacccgaa acaaaaatgt
    64141 gataccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    64201 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    64261 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    64321 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    64381 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    64441 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    64501 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    64561 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    64621 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    64681 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    64741 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    64801 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    64861 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    64921 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    64981 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    65041 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    65101 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    65161 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    65221 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    65281 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    65341 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    65401 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    65461 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    65521 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    65581 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    65641 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    65701 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    65761 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    65821 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    65881 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    65941 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    66001 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    66061 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    66121 acaccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    66181 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    66241 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    66301 ttattaccag cagcgcctat accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    66361 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    66421 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    66481 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    66541 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    66601 cctttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    66661 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    66721 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    66781 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    66841 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    66901 gtgtagaccg cgtctccggg ggatctgacg aaatgc
//
LOCUS       DACAPB010000004        63842 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.004, whole genome shotgun sequence.
ACCESSION   DACAPB010000004 DACAPB010000000
VERSION     DACAPB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.004"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..264
                     /locus_tag="IT904_000204"
     CDS             <1..264
                     /locus_tag="IT904_000204"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="catalyzes the hydrolysis of pyrophosphate to
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HAP3190044.1"
                     /translation="PVGVLKMTDEAGEDAKLIAVPHTKLSKEYDHIKDVNDLPELLKA
                     QIAHFFEHYKDLEKGKWVKVEGWENAEAAKAEIVASFERAKNK"
     gene            complement(344..694)
                     /gene="chpB"
                     /locus_tag="IT904_000205"
     CDS             complement(344..694)
                     /gene="chpB"
                     /locus_tag="IT904_000205"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HAP3190045.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(688..939)
                     /locus_tag="IT904_000206"
     CDS             complement(688..939)
                     /locus_tag="IT904_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ChpS family
                     antitoxin"
                     /protein_id="HAP3190046.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(1151..1492)
                     /locus_tag="IT904_000207"
     CDS             complement(1151..1492)
                     /locus_tag="IT904_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP3190047.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(1495..5274)
                     /gene="tamB"
                     /locus_tag="IT904_000208"
     CDS             complement(1495..5274)
                     /gene="tamB"
                     /locus_tag="IT904_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAP3190048.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(5271..7004)
                     /gene="tamA"
                     /locus_tag="IT904_000209"
     CDS             complement(5271..7004)
                     /gene="tamA"
                     /locus_tag="IT904_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAP3190049.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            7210..7848
                     /gene="msrA"
                     /locus_tag="IT904_000210"
     CDS             7210..7848
                     /gene="msrA"
                     /locus_tag="IT904_000210"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP3190050.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            8171..9514
                     /locus_tag="IT904_000211"
     CDS             8171..9514
                     /locus_tag="IT904_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP3190051.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(9576..9782)
                     /locus_tag="IT904_000212"
     CDS             complement(9576..9782)
                     /locus_tag="IT904_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAP3190052.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            10107..10664
                     /locus_tag="IT904_000213"
     CDS             10107..10664
                     /locus_tag="IT904_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP3190053.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(10654..11394)
                     /gene="cysQ"
                     /locus_tag="IT904_000214"
     CDS             complement(10654..11394)
                     /gene="cysQ"
                     /locus_tag="IT904_000214"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAP3190054.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPEIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVCKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            11584..13527
                     /gene="cpdB"
                     /locus_tag="IT904_000215"
     CDS             11584..13527
                     /gene="cpdB"
                     /locus_tag="IT904_000215"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAP3190055.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(13645..14025)
                     /locus_tag="IT904_000216"
     CDS             complement(13645..14025)
                     /locus_tag="IT904_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP3190056.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            14114..14974
                     /gene="qorB"
                     /locus_tag="IT904_000217"
     CDS             14114..14974
                     /gene="qorB"
                     /locus_tag="IT904_000217"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP3190057.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWALTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            15082..16047
                     /locus_tag="IT904_000218"
     CDS             15082..16047
                     /locus_tag="IT904_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP3190058.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            16155..16817
                     /gene="ytfE"
                     /locus_tag="IT904_000219"
     CDS             16155..16817
                     /gene="ytfE"
                     /locus_tag="IT904_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAP3190059.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(16862..18274)
                     /gene="cycA"
                     /locus_tag="IT904_000220"
                     /gene_synonym="dagA"
     CDS             complement(16862..18274)
                     /gene="cycA"
                     /locus_tag="IT904_000220"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAP3190060.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(18583..19203)
                     /locus_tag="IT904_000221"
     CDS             complement(18583..19203)
                     /locus_tag="IT904_000221"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="FKBP-type; rotamase; catalyzes the cis-trans
                     isomerization of proline imidic peptide bonds in
                     oligopeptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP3190061.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            19422..20060
                     /gene="ytfB"
                     /locus_tag="IT904_000222"
     CDS             19422..20060
                     /gene="ytfB"
                     /locus_tag="IT904_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAP3190062.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(20044..20691)
                     /locus_tag="IT904_000223"
     CDS             complement(20044..20691)
                     /locus_tag="IT904_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3190063.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWSDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            20904..21695
                     /locus_tag="IT904_000224"
     CDS             20904..21695
                     /locus_tag="IT904_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAP3190064.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            21705..22547
                     /locus_tag="IT904_000225"
     CDS             21705..22547
                     /locus_tag="IT904_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP3190065.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPQGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGNRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            22557..23333
                     /locus_tag="IT904_000226"
     CDS             22557..23333
                     /locus_tag="IT904_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAP3190066.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFNNQ"
     gene            23343..24884
                     /locus_tag="IT904_000227"
     CDS             23343..24884
                     /locus_tag="IT904_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAP3190067.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            24881..26962
                     /locus_tag="IT904_000228"
     CDS             24881..26962
                     /locus_tag="IT904_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP3190068.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            26983..27459
                     /locus_tag="IT904_000229"
     CDS             26983..27459
                     /locus_tag="IT904_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP3190069.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            27502..28801
                     /locus_tag="IT904_000230"
                     /pseudo
     CDS             27502..28801
                     /locus_tag="IT904_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
     gene            28854..29825
                     /locus_tag="IT904_000231"
     CDS             28854..29825
                     /locus_tag="IT904_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP3190070.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSQENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(29937..30386)
                     /gene="rplI"
                     /locus_tag="IT904_000232"
     CDS             complement(29937..30386)
                     /gene="rplI"
                     /locus_tag="IT904_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP3190071.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     ELFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(30428..30655)
                     /gene="rpsR"
                     /locus_tag="IT904_000233"
     CDS             complement(30428..30655)
                     /gene="rpsR"
                     /locus_tag="IT904_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP3190072.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(30660..30974)
                     /gene="priB"
                     /locus_tag="IT904_000234"
     CDS             complement(30660..30974)
                     /gene="priB"
                     /locus_tag="IT904_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAP3190073.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(30981..31376)
                     /gene="rpsF"
                     /locus_tag="IT904_000235"
     CDS             complement(30981..31376)
                     /gene="rpsF"
                     /locus_tag="IT904_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP3190074.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDADAGDSEE"
     gene            31704..31979
                     /locus_tag="IT904_000236"
     CDS             31704..31979
                     /locus_tag="IT904_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP3190075.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(32108..32794)
                     /locus_tag="IT904_000237"
     CDS             complement(32108..32794)
                     /locus_tag="IT904_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP3190076.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(32794..33648)
                     /locus_tag="IT904_000238"
     CDS             complement(32794..33648)
                     /locus_tag="IT904_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HAP3190077.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(33658..34308)
                     /gene="ulaD"
                     /locus_tag="IT904_000239"
     CDS             complement(33658..34308)
                     /gene="ulaD"
                     /locus_tag="IT904_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP3190078.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(34322..34786)
                     /gene="ulaC"
                     /locus_tag="IT904_000240"
     CDS             complement(34322..34786)
                     /gene="ulaC"
                     /locus_tag="IT904_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAP3190079.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(34796..35101)
                     /gene="ulaB"
                     /locus_tag="IT904_000241"
     CDS             complement(34796..35101)
                     /gene="ulaB"
                     /locus_tag="IT904_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAP3190080.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(35117..36514)
                     /gene="ulaA"
                     /locus_tag="IT904_000242"
     CDS             complement(35117..36514)
                     /gene="ulaA"
                     /locus_tag="IT904_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP3190081.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPSFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            36869..37933
                     /gene="ulaG"
                     /locus_tag="IT904_000243"
     CDS             36869..37933
                     /gene="ulaG"
                     /locus_tag="IT904_000243"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP3190082.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            38041..38796
                     /gene="ulaR"
                     /locus_tag="IT904_000244"
     CDS             38041..38796
                     /gene="ulaR"
                     /locus_tag="IT904_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAP3190083.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(38793..39542)
                     /gene="yjfP"
                     /locus_tag="IT904_000245"
     CDS             complement(38793..39542)
                     /gene="yjfP"
                     /locus_tag="IT904_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP3190084.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            39724..40053
                     /gene="bsmA"
                     /locus_tag="IT904_000246"
     CDS             39724..40053
                     /gene="bsmA"
                     /locus_tag="IT904_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAP3190085.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            40202..40477
                     /gene="yjfN"
                     /locus_tag="IT904_000247"
     CDS             40202..40477
                     /gene="yjfN"
                     /locus_tag="IT904_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAP3190086.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(40594..42219)
                     /locus_tag="IT904_000248"
     CDS             complement(40594..42219)
                     /locus_tag="IT904_000248"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAP3190087.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(42303..43466)
                     /locus_tag="IT904_000249"
     CDS             complement(42303..43466)
                     /locus_tag="IT904_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP3190088.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPLYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(43469..44107)
                     /locus_tag="IT904_000250"
     CDS             complement(43469..44107)
                     /locus_tag="IT904_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAP3190089.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            complement(44117..44515)
                     /locus_tag="IT904_000251"
     CDS             complement(44117..44515)
                     /locus_tag="IT904_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAP3190090.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(44533..45192)
                     /locus_tag="IT904_000252"
     CDS             complement(44533..45192)
                     /locus_tag="IT904_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HAP3190091.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIALGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(45243..45941)
                     /locus_tag="IT904_000253"
     CDS             complement(45243..45941)
                     /locus_tag="IT904_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HAP3190092.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITTEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(45960..46361)
                     /locus_tag="IT904_000254"
     CDS             complement(45960..46361)
                     /locus_tag="IT904_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAP3190093.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(46488..47219)
                     /gene="rlmB"
                     /locus_tag="IT904_000255"
     CDS             complement(46488..47219)
                     /gene="rlmB"
                     /locus_tag="IT904_000255"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP3190094.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(47399..49840)
                     /gene="rnr"
                     /locus_tag="IT904_000256"
     CDS             complement(47399..49840)
                     /gene="rnr"
                     /locus_tag="IT904_000256"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP3190095.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(49879..50304)
                     /gene="nsrR"
                     /locus_tag="IT904_000257"
     CDS             complement(49879..50304)
                     /gene="nsrR"
                     /locus_tag="IT904_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAP3190096.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLMELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(50509..51807)
                     /gene="purA"
                     /locus_tag="IT904_000258"
     CDS             complement(50509..51807)
                     /gene="purA"
                     /locus_tag="IT904_000258"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP3190097.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(51911..52108)
                     /locus_tag="IT904_000259"
     CDS             complement(51911..52108)
                     /locus_tag="IT904_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAP3190098.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(52190..53194)
                     /gene="hflC"
                     /locus_tag="IT904_000260"
     CDS             complement(52190..53194)
                     /gene="hflC"
                     /locus_tag="IT904_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAP3190099.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(53197..54456)
                     /gene="hflK"
                     /locus_tag="IT904_000261"
     CDS             complement(53197..54456)
                     /gene="hflK"
                     /locus_tag="IT904_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAP3190100.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSASQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(54542..55822)
                     /gene="hflX"
                     /locus_tag="IT904_000262"
     CDS             complement(54542..55822)
                     /gene="hflX"
                     /locus_tag="IT904_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP3190101.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDLEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(55899..56207)
                     /gene="hfq"
                     /locus_tag="IT904_000263"
     CDS             complement(55899..56207)
                     /gene="hfq"
                     /locus_tag="IT904_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAP3190102.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(56293..57243)
                     /gene="miaA"
                     /locus_tag="IT904_000264"
     CDS             complement(56293..57243)
                     /gene="miaA"
                     /locus_tag="IT904_000264"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP3190103.1"
                     /translation="MSDISKAGLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMAYITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(57236..59083)
                     /gene="mutL"
                     /locus_tag="IT904_000265"
     CDS             complement(57236..59083)
                     /gene="mutL"
                     /locus_tag="IT904_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP3190104.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLCQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFESGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(59093..60427)
                     /gene="amiB"
                     /locus_tag="IT904_000266"
     CDS             complement(59093..60427)
                     /gene="amiB"
                     /locus_tag="IT904_000266"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAP3190105.1"
                     /translation="MMYRIRNWLVATLLLCTPVGAATLSDIQVSNGNQQARITLSFIG
                     DPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLT
                     ENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNPF
                     KTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVT
                     IAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRS
                     ATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFG
                     HSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEER
                     LLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(60446..60907)
                     /gene="tsaE"
                     /locus_tag="IT904_000267"
     CDS             complement(60446..60907)
                     /gene="tsaE"
                     /locus_tag="IT904_000267"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP3190106.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(60879..62426)
                     /gene="nnr"
                     /locus_tag="IT904_000268"
     CDS             complement(60879..62426)
                     /gene="nnr"
                     /locus_tag="IT904_000268"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAP3190107.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            62425..63564
                     /gene="queG"
                     /locus_tag="IT904_000269"
     CDS             62425..63564
                     /gene="queG"
                     /locus_tag="IT904_000269"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP3190108.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(63547..63600)
                     /locus_tag="IT904_000270"
     CDS             complement(63547..63600)
                     /locus_tag="IT904_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190109.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT        15429 a        17126 c        15863 g        15424 t
ORIGIN      
        1 ccggttggcg ttctgaaaat gaccgacgaa gccggtgaag atgcgaaact gattgctgtt
       61 ccgcacacca agctgagcaa agaatacgat cacattaaag acgttaacga tctgccagag
      121 ctgctgaaag cgcagatcgc tcacttcttc gagcactaca aagacctcga aaaaggcaag
      181 tgggtgaaag ttgaaggttg ggaaaacgca gaagccgcta aagctgaaat cgttgcctcc
      241 ttcgagcgcg caaagaataa ataagttctt atagcgcaat aaccctgaac accgggcttc
      301 ggttagtaag ggttttttta tgcccgcgat aaataaacac accttattcc accaccgcct
      361 gcaagcgtaa taacgcctct tccaccacct catccgcagc cagaccaata cgctttgcca
      421 gccgggcgcg tagatccatc atccgcacct gattaaccag caccacgccg tgcacatcgc
      481 cttcttcgca atgtaaagga acgctaaatc cggcataacg ggcaaaattt ccaccctgcg
      541 taatgggggc caccagcgtc attcccagtt gattaaaggc ttgaacggag agcacaagcg
      601 caggtcgacc agcaccttgc tgttcatggc cgcttgctgg atcaaagcca accagcacaa
      661 tgtctccccg ttcaaattca cttttcttta ccatatttcg tcacccgcag gggtggattt
      721 accccagaca tcctgctcgc taagttccgc ggcgttcatg tcacactgtg ccagcagttc
      781 atcaagcgag tagcgcctgg agatgggtgt cagaatcagt tggttgttgc tcacctgcgc
      841 ttccacgctc tgccccggct gtaagttaag ttctttcatt acgatattgg gaatgaccat
      901 acctgcactg ttcccccatc tttttatggt aatacgcatc tgcacctcct taagttatac
      961 aaagtataac tcacggtgct acaggcaacg ttttacccct gcgcatttgt gaacgccagc
     1021 agagtttttt atgcgttaat agatatggaa agcggctcgg agagaaaagc aaaaggtgag
     1081 ggaattacaa aacagaatgc gagtcgtctc gcaaaaaaca acgccacccg aaggtggcgt
     1141 gcttttatta ttacttatcc ctgtctaacc agtcgccgct ttcaattagc tttaatccat
     1201 cgacgggtcg ttgataaacg tacatccatg cactcccgta cggcgtctga atcaactggc
     1261 gcgcgtattc accgcccctg gtgcgcaagg catcaagttc ggccagcgtg gcgttgtcaa
     1321 tacgataaac ttcaccgtgt actgttccgt tccccggaac tgcgcctgga tagtggccca
     1381 ggctatacaa ctggtagtta tcgatactga aatcgcccag taactgggca ttggtcatcc
     1441 agtgactgtt gccttgtttg tggcgtaaac tgccgtagac aaatattcgc attgctaaaa
     1501 ctcgaactga tagagcaaat ccagtgcctg gtctacacca gacacggctt ccagatatag
     1561 cttaggcatc aggcgataac gtaacgtgag tgttgctata gagtcaaata tacccacgcc
     1621 gtatttcact tgcagacctg gcaatacata gccgctgacc actacctggg aggagtcgcc
     1681 tactccctgg gtgtcgagcg ctaaattgct tacgccaaac gtctcgccga ttttacccac
     1741 aatctggcca ctttgcgcaa cccccagacc aatcagcatc gaggtcattg ccgcactgtc
     1801 gctctgatcg ctctccagcc cttgtccacg tagcaaataa gacaatgcag cttgttgcga
     1861 catcgccggg tcagagaaga tctccgcttt cggttcgtcc gccagaccag tgacgcgaac
     1921 tccggcgatt acgtcgtctt ctgtagcatc cgggttacga atagcttcaa tattaagata
     1981 cggttgatcc ggcggaccag agaacagtaa ctcaccctta cgcacaatca gatcctgacc
     2041 ataggcatgg aagcgccctt cagggatgtt gatctgcccg ttcaggccca gcccttgttt
     2101 gtcctgaacg acgttaagat cgcccgtcag ccgcgctttc aggccaaagg cgtcaatgcg
     2161 cacattgttg ccaacgtgga caatcaggtt actgttaatc ggaatcgacg ccgttttcgg
     2221 ctcttccggt tgcaggttat cgttaagcat caccacatcg ctggagacgc ctactgcgct
     2281 ttccggcaga tcgtgcacca cgatgcgcgc ccaagggaca tccacgcgac catcgagggt
     2341 aaacaggttt ggtgtagcct cgaatacaac atctggcgat acatccattc gtaccatcgg
     2401 cggcacggtg atccgcactt tactgccttt cgccgttacc cgcgcccgcc agttttcaat
     2461 ttggctccag tcggcatcac cgttcaggta gatttcaccc tgctgggtcc gtactgtacc
     2521 ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc tggctcggtt gcatatcaaa
     2581 cggcataaag ttgccatcga tatccacacc cgtaacctga agctgaccaa acaactgcgg
     2641 gctttgcaca tcaccaccca gacgcaagtt ggcactcacc atccccgctg ctttttcccc
     2701 acgggtaaag atggggttta tcatcgcaag gttgaagtta cggatattga cgttgccacc
     2761 aagattacgg cggccttgcg gatcggtcac ctgcacctgt ccatcaaact ggccgttatt
     2821 ggtcaggcgg atggtccagc ccaattcggc acggttgtta cgcaattccg ccgtcagatt
     2881 cagtgtctga aacgccacgg gcagcgccgc atcgttgacg gtttgcgtta cctgcacgtt
     2941 acgccccgaa agggtgatac tgccctgcgg cagcccctct ttcgtggtgt cccaggcaac
     3001 atccgcttta cccgtgaaga taccgctggc ctgagtggtt tctggcataa acggtttcag
     3061 catggcgagg tcgaagcggt tgagattcac caccgcacgc ccttcggccc ccgcatcgat
     3121 agtttgcggc acgcacagtt ccgcattcgg gttaagccag cagtgtggcc cgatgctgat
     3181 tttttgctcc ttattgcggt aatccagcgc aatatcgcgg gtcagcgacc acgggccgac
     3241 cggcgtctgg aagcgggtat tgctaagagt tcccttccag cgttcttctt tgcgatcaaa
     3301 acttcctgcc agattaagct gcccggagac aggctcgccc tgaatccgca actgtagctc
     3361 gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg ttgatattga catccggctg
     3421 cgaaatttgc tcaacgcgta cgtcgagttt acccgcaatc tgatcggtgg atttgatatc
     3481 gccttccacg cgaacctgcg ccacggaaag ttcctgccag cgcaggccgc gcgcggtgat
     3541 atctgccagt agttgtggcg cttccaccgt gccgcgtact tttaccagcc ctttcgctgt
     3601 accgccaagc cccggcagcg cgttatccag acccggcgcg ttgatggtgg catcaagatt
     3661 gagatctttt acccccagct cgcctttcac ttcggcactg tttggcccca gttccagatg
     3721 cagccctggg atcatccact gcatataact gttgcctttc agcgtgccgt caacgttcac
     3781 tttgttctgt ttgacgttac cggtcagctt cagttctggc acctccattt gccaggtgcc
     3841 gccgtacagg ctaccgcggg ttttaatcaa gccattgagt ttcgacggcc actctgggat
     3901 ctctttggcg gtgttaatgc cgttaagcgt tagctcaccg cgccaactaa tggcctgctg
     3961 ccagtcgagc aacgccttga gttcagtttt cccttccagc gccgcgacgg tgagtttgtc
     4021 gagattgacc tgctgttcat tacctttggc atcaagggta atggtggctg gcgggatctc
     4081 cagtcccttc actgccgtac gcatagagag cgtgtaatcg gtcattttgc cggtaagttt
     4141 cagtttcaga tcatccgcct gatactgctt ctcaccagtg aacggccagt aaatctgttt
     4201 gctgttcact tccacgttga gcggcaatcc ggcttccgcc agtcgcgtct gggcgcgtaa
     4261 atccatatcc accggaccgg aaagattaac gccaatctcc agctgttcac gcagcgcgcc
     4321 gcccactttt agcttcactt tttcaccttt caacggctcc acgttcagtg tgctgttgag
     4381 ggtgatatcc accggccagt tgtctgacag ctgcgccgtg ccgctggcgt tgacgatccc
     4441 ctggttggaa tcgatatcca gggcgtccag tttagtattg ccgtcaatgc tgctcacttt
     4501 cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc acgcgtaact gctcgccttt
     4561 aaactcttca atgttcaggt taagcggcaa atgcacgtcg gtcatttccg gcaataccgg
     4621 gcgagaaaag agatctttca gcgtttcgcc gagcggcttt tcatccggct gcggattttc
     4681 aatcttcggt tcgacgacct cttcctgcgc cacttccgcc actttcggca gagcaatcag
     4741 caggcctttc agcgacgtcg gtttcagggt cagggttttc tcctgccagt tcaggccgga
     4801 ggtgaagtcc atcaccgata ccgtggtgtc atcaatcttg atgttgacgt tatccagcgc
     4861 cacccgtgtc agggtgatgg gatacggcgt ggagagatcc agcggaccgc tatcttcctc
     4921 ttcttcaacc tgttcagaag gaggcatttt tttactgtcg atgttgacct gaatgtcttt
     4981 cagcgccagg tcattaatac aaacgctact gttccacagg cactcaagcc cgaccgccag
     5041 atgcagattg cccgctttta ccgccacgcc tggctgctca taacgaacgt cagacaaggt
     5101 gagatcgcgc cagccgccgg tgaccttgcc aatatccagt cctggtaccc agcgatccgc
     5161 cgctttaaat accagatgca ggccactggt ggtgcccacc agaaacgcca ccgatcccag
     5221 caacagtaag ataacgataa ccacgccgag gctgattttt ttccataaac tcataattct
     5281 ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt tatccgcgac cggtacggca
     5341 aaatcgagtt tgattggccc gaccggcgat tcccagcgca cgccgacccc ggtaccggtt
     5401 ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac tatcgacaaa caccgcgccc
     5461 caccattttc cggtcacgtt gtactggtac tccagcgatc cggttatcaa cttcgaggcc
     5521 cctttcaggt caccgttggc gtatttcgga gcgatagatt tgtatttgta gccacgaata
     5581 ctgcggtcgc ccccggcgaa gaaacgcaga tccggcggta ctttgtcgaa atcaccggtt
     5641 tcaatccagc ccagcgtgcc gcgtgtaaca aaacgatggc gatcgtacag tgtgcggatc
     5701 cagacgtttt gtgcctggaa aacggagaaa tcgacatctg agccccaggc cgtgttggag
     5761 tagtcgatag agtagcgttg cgagtcgccc caggttggca tcaggccacc acgagaacgc
     5821 gtgcggctaa tcatcacccc aggataaaac agcatcgtgg tgttggtaat ttcaccctga
     5881 gtaaagtggt cgagactcca gcgcaggtta atggcacgct gccagccgct ggagagatcc
     5941 cagtagcgag aagccaccag cgtagtggag tcagattcgg tatcgttcag gtcagtgcgc
     6001 ttaaaaccgc cctgcaccaa ataatattgt tccagtggat tcttcagcag tggcatttta
     6061 tagctgaagt cgagggtctg ttccggcgcg gaaatactgg tactggtggt caaactgtga
     6121 ccgtatgagt tcatccacgg ctttttccac gtcgctttca cgcgcggtcc cacgtccgta
     6181 gagtaaccga ccccggtttc gatggtgttt tctgttcgcg gcgaaaccac gcccgtcaat
     6241 ggtaatactt tcgtttcgcg cgctttatca aattgtggag ccaccaccac cgagttaaac
     6301 cagccggtag cagaaagtcg gcggttcagt tccgccagat ctttcgattc gtactcatcg
     6361 ccctctttaa acggcaccag attttgcagg tattcatcgc ggatttgtga tccttcaaag
     6421 gtcacatgcc caaagcggta acgttcgcca ctgttataat caatatccca gaaggcttta
     6481 tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt cgctatcgaa ataaccttta
     6541 cgcaacgcaa tgctggttaa ggactttttg aaattttcat aatcgccctg gttcagcacc
     6601 gtgccaatag ccgggcgagt atcgagcaat ttcaaatagt ctttatcggt ccgcgcgccg
     6661 ccgcgcaata ccacatcggt gccgccaatt aacaccggca cgcctggcgt gactttggcg
     6721 atcaatacct gccgcccttt ctttggcggt ggacggagat caaattcaat ggtcggctgg
     6781 taataaccca gcgctttcag accttcgcgg atggcatcat cgacgcgtgc gcgaaagcga
     6841 cggtctggcg tcacttcatc actttcaatc gtagaaagct gcgcacgaac gttcttttcc
     6901 agctgtcccg ataacccctc gacctgtaga cggacgttcg cggcgacggc agatccgctt
     6961 aagcagagta agcttacaca gcataactgt cggatatagc gcacattttc tcctgaatat
     7021 ccttttttcc tgcccctgga acgccgttaa acggcttaac aaaaatccag taatatggat
     7081 taaaaagcag actaaacccc aaatatttct tatgttttac tttagaccta ttcacggtgg
     7141 ttattgtgtg caaatacgcc tcttgttaca accttaaccc caatgaccga ttttcgggag
     7201 agcgacacca tgagtttatt tgataaaaag catctggttt cccccgccga tgccctgcct
     7261 ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg tcaacggtca ctcaatgacc
     7321 aatgtacctg acggaatgga gattgccatt tttgcgatgg gttgtttctg gggtgtggag
     7381 cgtctgttct ggcagttacc cggcgtttac agcaccgccg caggctatac cggaggctat
     7441 acgccaaatc cgacttatcg ggaagtgtgc tccggtgata cgggtcatgc cgaagcggta
     7501 cgcattgttt acgatccttc cgtcatcagc tatgagcagt tgctacaggt attttgggag
     7561 aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca gtatcgttca
     7621 gcgatttatc cgctgacccc agaacaggat gccgcagctc gcgccagtct ggaacgtttt
     7681 caggcggcga tgcttgccgc cgatgatgat cgtcacatca ccacggaaat cgctaacgcc
     7741 acaccgtttt attatgccga agatgaccac cagcaatatc tgcataaaaa cccgtatggt
     7801 tactgtggaa ttggcggaat tggcgtctgt ctgccaccgg aagcatagcg ttacgggtac
     7861 aaatgtagat tgttgataaa gtgcgcttta tttatgccag atgcggcgta aacgccttac
     7921 caggcctaca aaatcgtcca aattcaatat attgcaagga ctgcgtaggc ctgattggca
     7981 tagcgcatca ggcagtattg catttatcag cagtctgaat tttaaccctc tggcgacttt
     8041 acagtacctt acgctatact agccactgaa aatgccggtt cactttcttc gaatcggctt
     8101 tcaatgtgta tttcacacaa attaatcaac ttccccttcc gaggatctgg cctgaaaggt
     8161 cggataagat atgttaaaca gtattttagt catactctgc ttgatcgctg taagtgcgtt
     8221 cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa atcaaactta aactgctggc
     8281 tgatgaaggc aatataaatg cccaacgcgt tctgaatatg caggaaaatc ccggcatgtt
     8341 ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga
     8401 tgcggcattt tctccagctt ttcacagcct gttctcccgc tatatgtcgg cagaactctc
     8461 tgagcaactg agctttattc tctctttctc gttagtgact ggcatgttta tcctgtttgc
     8521 ggatttaact ccgaaacgca tcggtatgat tgcgccagaa gcggtggctt tgcgtatcat
     8581 caacccgatg cgcttctgcc tgtacgtttg caccccgctg gtgtggttct tcaacggcct
     8641 ggcgaacatg atcttccgta ttttcaaact gccaatggta cgtaaagatg acatcacttc
     8701 tgatgacatc tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga
     8761 acacgagctg attgaaaacg tctttgagct ggaatcccgt accgttccgt cttcaatgac
     8821 accgcgtgaa aacgtgattt ggtttgatct ccacgaagat gagcaaagtc tgaagaataa
     8881 ggtggcggaa catccgcact ctaagttcct cgtctgtaat gaagatattg accacatcat
     8941 cggttatgtc gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact
     9001 gaacagcggc gtacaaattc gcaacacgct gattgtgccg gatacgttaa ccctttcaga
     9061 ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg gtgatcatga acgagtacgc
     9121 gctggtggtg gggatcatca ccctcaacga cgtgatgacc acgctgatgg gcgatctggt
     9181 cggtcagggg ctggaagagc agattgtcgc ccgtgatgag aactcatggc tgattgacgg
     9241 cggcaccccg attgacgacg tcatgcgcgt gctggatatt gacgagttcc cgcagtcggg
     9301 caactacgaa accatcggcg gctttatgat gtttatgctg cgtaagatcc cgaaacgcac
     9361 cgattcggtg aaattcgccg gctacaaatt tgaagtggtg gatatcgata actaccgtat
     9421 cgaccaactg ctggtgaccc ggatcgacag caaggccacc gccctttcgc caaaactgcc
     9481 tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca gaaacatcaa cggctcctga
     9541 atcaggagcc gtcttattac tgcatagcac tttggttaag ccatctctgt ttgcagacgc
     9601 ataacctgac ggttaacttc ggacatcact gacaaatgca gcttatcctt cacttttggg
     9661 ataagaatct tacccttatc aaattcaaaa gcgccaacgt ccttgatgta taaccgtcca
     9721 cggaacagga tctttacgta cttcgccacc tgaagtgggt tgtaccgttg gaaaattttc
     9781 attgttatct cctgctgagt attacgcctg tgcggtgcca caatcggccc aactattatg
     9841 aggcgcaaat tttaatgctt agtgactata gactatccgg gcaatgtttc caccgcgtat
     9901 aacttttttt acctaaaggt tacaattatt cagaattatc tttttaccga agcgcggtct
     9961 tcagtataag cattcatttt tcatatgatt tgtgcgcttg accgcaaact ggcatcacac
    10021 ttgcgggaaa ttcgataaat agcacatatg attaaaactc agacccaagt ggtcggatca
    10081 cctgcatatc ataagaagga aacaccatga ccctacgcaa gattctggca ctcacctgcc
    10141 tgctgttgcc gatgatggct tccgcacatc agttcgaaac cggtcagcga gtgccgccga
    10201 ttggcattac cgatcggggc gagttggtgc ttgataaaga tcagtttagc tacaaaacct
    10261 ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca acatattgct ggtcgcacct
    10321 ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa atcagcgaag ttaccgcacg
    10381 atcgttacca gaccaccacc attgttaaca ccgacgacgc aattccgggt tccggcatgt
    10441 ttgtgcgcag cagtctggag agtaataaaa agctttatcc ctggtcgcag tttattgttg
    10501 atagcaatgg cgtcgcacgc ggtgcctggc agctggatga aaaaagttcc gctgtagtgg
    10561 tgctggataa agacggtcgc gtgcaatggg ccaaagacgg ggcgcttact caggaagagg
    10621 tgcagcaagt gatggacctg ctgcataaat taattaataa atagacactc tgaaccccgg
    10681 attcaggaac gactcgcgcg gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg
    10741 cgctccggca gctgcagcca cagcatgtcc agcggcggtg tcccaaatat tcgttggtcc
    10801 gaagcgcggg tacagctgcg cctgtccttc cgccaccagg cagaatttca gcgaagagcc
    10861 gatggacgtg gtctgatgtt cgccaagctg ttgcagatac tctttcagct ccgcatccgc
    10921 atgggatcgg ctaataacga ccagtggcgg gcgcgcatcg cggacctgaa tctgcttgca
    10981 cacgccgcac tcttctttcc acgctttgcc ttctgccgcg ctgtacatta cgttcattac
    11041 cggcgcatac accacgccta aaatcggttt gccatggtca atgagcgcaa tgttaacggt
    11101 gaattcgcca ttacgtttaa taaactcttt agtgccatcc agcgggtcca ccagccagta
    11161 acgctgccag tgctgacgga cttcccaacc gggaggatct tcttcagaaa ggaccggaat
    11221 ctccggtgtc agcgtacgta aaccgtccat gataacggtg tgtgcggcaa tatccgctgc
    11281 cgttaccgga gaattgtccg ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac
    11341 ctgcataatg gcatcgcctg cattccgtgc aagctggcat acttgatcta acatttctcc
    11401 acctcgtctc tgtgagcggt gttaacttat tgttttactt ataccctatc gttaatgaat
    11461 gcgccaactg tgatagtgtc atcattttca aagcgtaaaa ttgtggcatt cttcactgtt
    11521 ctataagtaa ggcgtttatt ctcccttttc tttcgtattc ccgatgataa aaggatgtcc
    11581 ctgatgatta agtttagcgc aacgctcctg gccacgctga ttgccgccag tgtgaatgca
    11641 gcgacggtcg atctgcgtat catggaaacc actgatctgc atagcaacat gatggatttc
    11701 gattattaca aagacaccgc cacggaaaaa ttcggactgg tacgtacggc aagcctgatt
    11761 aacgatgccc gcaatgaagt gaaaaacagc gtactggtcg ataacggcga tttgattcag
    11821 gggagtccgc tggccgatta catgtcggcg aaaggattaa aagcaggtga tattcacccg
    11881 gtctataagg cattaaatac gctggactat accgtcggca cgcttggcaa ccatgagttt
    11941 aactacggtc tggattacct gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat
    12001 gccaacgtca ttgacgccag aaccaaacag ccaatgttta caccgtattt aattaaagat
    12061 accgaagtgg tcgataaaga cggaaaaaaa cagacgctga agattggcta tattggcgtc
    12121 gtgccgccgc aaatcatggg ctgggataaa gctaatttat ccggaaaagt gacggtgaat
    12181 gatattaccg aaaccgtgcg caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc
    12241 gttgtcgttc tggcacattc cgggctatct gccgatccgt ataaagtgat ggcggaaaac
    12301 tcagtttatt acctcagtga aattccgggc gttaacgcca ttatgtttgg tcatgctcac
    12361 gccgttttcc cgggtaaaga ttttgctgat atcgaagggg ctgatatcac caaaggcacg
    12421 ctgaatggtg ttccggcggt aatgccgggc atgtggggcg atcatcttgg ggtggtcgac
    12481 ttacaactca gtaatgacag cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg
    12541 ccgatttacg atatcgctaa taaaaaatca ctcgcagcgg aagacagcaa gctggtagaa
    12601 acactcaaag ccgatcacga tgccacacgc cagttcgtca gcaagccaat cggtaaatct
    12661 gccgacaata tgtatagcta tctggcgctg gtgcaggacg atccgaccgt gcaggtggtg
    12721 aacaacgcgc aaaaagcgta tgtcgagcat tacattcagg gcgatccgga tctggcaaaa
    12781 ctgccggtgc tttcagctgc cgcaccgttt aaagtcggtg gtcgcaaaaa tgacccggca
    12841 agctatgtgg aggtggaaaa aggccagttg accttccgta atgccgccga tctttatctc
    12901 tatcccaata cgctgattgt ggtgaaagcc agcggtaaag aggtgaaaga gtggctggag
    12961 tgctccgccg gacagtttaa ccagattgat cccaacagca cgaaaccaca gtcactcatc
    13021 aactgggatg gtttccgcac ttataacttt gatgttattg atggtgtgaa ttatcagatt
    13081 gatgttaccc agcccgcccg ttatgacggc gagtgccaga tgattaatgc caatgcggaa
    13141 aggattaaga acctgacctt taatggcaag ccgattgatc cgaacgctat gttcctcgtt
    13201 gccaccaata actatcgcgc ttacggcggc aaatttgccg gtacgggcga cagccatatc
    13261 gcttttgctt caccggatga gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg
    13321 aaacgtgcgg gggaaattca cccggcggca gataacaact ggcgtttagc accgatagct
    13381 gccgataaga aactggatat ccgtttcgaa acttccccgt cagataaagc cgcagcgttt
    13441 attaaagaga aagggcagta tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg
    13501 atttatcagg tggatttgag taagtaaaac acttcttttt cggcctataa atcatcaatc
    13561 gcatccggca tttattggcg gatgcgatgc tggcgcatct tatccgccct acaagtcata
    13621 caccgtaggc cgcgcatccg gcagttatgc cgcacgttca tcccgcaccg ccaacacctc
    13681 gggcaaattc aactcaatcc agtccgccag tgcagcaacc ttttcgctca cctgctcgcc
    13741 cagcggcgtg aggctgtatt caacatgcgg tggcaccacc ggatacgcga tacggttaag
    13801 aaaaccatcc tgttctaacg cctgtaacga ctgcgcaagc atcttttcgc tcaccccgcc
    13861 gattttgcgc cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa
    13921 caccccccaa cggctggtga cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa
    13981 gagattaccc tctttcagtt gttgcgacag gctaacctga ctcatttcat acttaccttt
    14041 ttgtgcgtac ttactaaaag taagtttagg tgttagcgta tttaaacaca agacaaaacg
    14101 atggagactt cccatgatcg ctattactgg tgccactggc caacttggtc agtatgttat
    14161 tgaatccttg atgaaaacgg ttcctgccag ccaaatagtg gctatcgttc gtaatccggc
    14221 aaaagcccag gcactgacag cacaaggcat taccgtgcgt caggctgact acggcgatga
    14281 agccgcactg acatctgcac ttcagggagt ggaaaaacta ctgctgatct cttccagcga
    14341 agtgggtcaa cgtgccccgc agcatcgtaa tgttattaat gccgcaaaga cggctggcgt
    14401 gaaatttatc gcttatacca gcctgcttca cgcggataaa tctccgctcg gcctcgccga
    14461 tgagcacatc gagacggaga aaatgctggc tgattctggc atcgtttaca ccctactgcg
    14521 caacggctgg tacaccgaaa actacctcgc cagcgccccg gcagcactgg aacacggcgt
    14581 atttatcggt gcggcgggcg atggcaaaat cgcctcagca acgcgggcag attatgcggc
    14641 agctgcggca cgcgtgatta gcgaagccgg tcacgaaggc aaggtttacg aactggcggg
    14701 cgatagtgcc tgggcgttga cacagttagc ggcagagctg accaaacaga gcggaaaatc
    14761 cgttacctat caaaatctga gcgaagccga tttcgccgcg gcactgaaaa gcgtcggact
    14821 gcccgacgga ctggcggata tgctggcgga ttctgacgtt ggcgcatcga aaggtggtct
    14881 gtttgataac agcaaaacgc ttagcaaatt gattggccgc ccaacgacaa cgttagccga
    14941 aagcgtaagc catcttttta atgttaataa ctagttaatt aaagtggcat cctcccgcat
    15001 cctctctgat aatgacggga tgccgggagc aatcatgtct gcttcctgaa ctttcttctg
    15061 acagaccaat ggatgccagt aatgattagc ggcgtgctgt acgccctgtt agcagggttg
    15121 atgtgggggc ttatttttgt cgggccgttg atcgtaccgg aatacccggc gatgttgcag
    15181 tcgatggggc gttatctggc gttagggtta attgcgctgc ccattgcctg gctgggacgc
    15241 gtgcgtctgc gtcagttggc gcgtcgggac tggcttaccg ccttgatgct cactatgatg
    15301 ggcaacctca tttattactt ctgccttgcc agtgccattc aacgtactgg cgcgcctgtt
    15361 tccacgatga ttatcggcac cctgccggtg gtcattcctg tctttgccaa tctgctttat
    15421 agccagcgcg acggcaaact cgcgtgggga aaactcgccc cggcactgat ttgtattggc
    15481 atcggcctgg cgagtgtgaa tattgctgag ttaaaccacg gactccccga ttttgactgg
    15541 gcacgttata cctctggcat cgtgctagcg ttagtttccg tggtctgctg ggcatggtat
    15601 gccctgcgca acgcccgctg gctgcgggaa aatcccgaca aacatccgat gatgtgggcg
    15661 acggcgcagg cgctggtcac gctgccggtt tctctcatcg gctatctcgt cgcctgttac
    15721 tggctgaata tacaaacgcc ggacttctcc ttaccttttg gcccccgtcc gctggtgttt
    15781 attagtctga tggttgcgat agccgtgctt tgctcatggg ttggcgcact ctgctggaac
    15841 gtcgccagcc agcgattacc gacagtgatt ctcgggccgc tgattgtttt cgaaacgctg
    15901 gcaggtttgc tgtacacctt tttactccgc cagcaaatgc cgccgctaat gacgctgagc
    15961 ggtatcgcgc tgttagtgat tggcgtggtc attgcggtca gagcaaaacc agaaaaacct
    16021 ttaactgaat ctgtctcaga aagttgacac gctggcagtg agttaaataa gcctctgcta
    16081 cgtaagggtt atagcttttg ccttaaagat gcatttaaaa tacatcttat cttattaaga
    16141 atgaggtatc agctatggct tatcgcgacc aacctttagg tgaactggcg ctctctatcc
    16201 ctcgcgcttc ggctctgttt cgtaaatatg atatggatta ctgctgtggc ggtaagcaga
    16261 cgctggcgcg cgcggcggca cgtaaagaac tggatgttga ggtcattgaa gctgaactgg
    16321 caaagcttgc tgaacaaccg attgagaaag actggcgtag cgccccgctg gcagaaatca
    16381 ttgaccatat catcgtgcgc taccacgatc gtcaccgcga gcagctgccg gagctgatcc
    16441 tgcaagcgac taaagtcgag cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga
    16501 caaaatacct gaccatgctg catgaagagc tttccagcca catgatgaaa gaagagcaga
    16561 tcctcttccc gatgatcaaa caaggcatgg gcagccaggc aatggggcca atcagcgtaa
    16621 tggaaagcga gcacgatgaa gcgggcgaac tgctggaagt gatcaaacac actaccaata
    16681 acgtcacacc gccgccagaa gcctgcacca cctggaaagc gatgtataac ggcattaatg
    16741 aactgattga tgacctgatg gatcacatca gtctggaaaa caatgtactg ttcccacgcg
    16801 cgctggcggg tgagtaatct aaaagctgga tggcattgcg ccatccagca tgataatgcg
    16861 gttatttccg cagttcagca gcccgcttct taccaataaa caaccagccc agccccagtg
    16921 cgataaacca cagcggggtg accaacagcg cctggcgagt gtcatcttcc agtgtcagca
    16981 acacgaccac gaacacaaag aacgccatac atacccagca catcagcttg ccgagcggca
    17041 tcttgtagat cgacttctca tgcagatgag gacgctgttt gcggtaaacc aggtacgagc
    17101 aaaggataat tgtccagacg aacataaaca gaatcgcgga aacggttgta atcatcgtga
    17161 acgcgccaat cacgctagga ttcacataca acatcaccac gccgccgagc agacagatac
    17221 acgagaaggt caaccctttc gcgggtactg cgcgcttaga aagtttagcg aacgctttcg
    17281 gtgctacacc ttcctgcgcc agaccaaaca gcatacggct ggtagagaag acgccgctgt
    17341 tagcggaaga cgccgcagag gtcagcacca caaagttgat cacgctggca gccgcaggca
    17401 aacctaccaa cacaaacagt tcaacaaacg ggctcttctc cgggactacc gaactccacg
    17461 gcgtcacgga cataatcaca atcagcgcga agacgtagaa cataatgata cggatcggaa
    17521 tggagttaat cgcgcgtggc agtgatttct ccggatcttt ggtttccgca gctgttgtac
    17581 ctaccagctc aatccccacg aaagcgaaaa ccgctatctg gaatccggca aagaagccac
    17641 ttaaaccttt cgggaaccag ccgccgtcat tccacaaatg cgcgaatgac gcttccacac
    17701 cggtcggtga ctgaaagtgc atcgccacca tgaccaggcc gacgacaatc agcgacacga
    17761 tggcgacgat tttgatcatc gcaaaccaga actccatctc accgaacatt ttcacggtgg
    17821 caaggttgag cgtcaacagc agcactatca ccgccagcga ggcgacccag tcggagagat
    17881 cggggaacca gaactgagca taagcagtga tcgccaccac gtctgccata ccggttacaa
    17941 cccagcagaa ccagtaagtc cagccggtga aatatcctgc ccacggcccg agtaaatcgg
    18001 aagcgaagtc actaaaagat ttgtattcca gattcgaaag cagcaattcc cccattgccc
    18061 gcatcacgaa aaagagcata aaaccgatga tcatataaac gaaaatgatc gacggcccgg
    18121 caaggctaat cgttttgcca gaccccataa acaaccccgt accaatggca ccgccaatgg
    18181 caataagctg aatatgtcgg tttgtgagat tgcgccgtag cgactgttca gccggagcct
    18241 gatcatcggc aacgactttt acctgatcta ccatgttttt ttcttcctgt acctgtctgt
    18301 gttgttcagg ctctacggcc tttagtcagt ctatgatacg acgaatctgt atcgggactc
    18361 atcgatatta ggtaacaata cgcgggatga atactaagat ttagatctaa tgttaatttt
    18421 atgtttaaag tgagtgtcat atcacggtga taacgcgaaa cagctcacaa aaatacacgc
    18481 ttacggggtc tttgcaacac aaaatagcgc cgtttggcaa actgaagggt ttattgctga
    18541 atgcctgctc ccctctggtt cgaggggaac agaatgcgct gcttagagga tttccagcag
    18601 ttcgacttca aacaccaggg tgctgaacgg agggatggat gcgcctgcgc cgcgctcgcc
    18661 atatgccagt tcctgcggga tagtcagttc ccatttagaa cctaccggca tcagagtcag
    18721 tgcttcaatc cagccaggga tcacgccatt aaccgggaat tcagcgggtt caccacgagc
    18781 aacggagctg tcaaacacgg tgccgtcgat cagtttaccg gtgtaatgaa cacgaacgcg
    18841 gtcagtacgt gccggaattg cgccttcacc ctggttgatc acgcggaatt gcaggccaga
    18901 ttcggtgctg ttcacacctt cttttttggc attttcttca agatatttca caccttcggc
    18961 agccatcgcc tggaaacgct gacgacgaac ggcatcggcg cgttcgtgga tttcacgcag
    19021 cgcgcgatgc accacatcaa ccggaacagc cggatgtttg ccttccagcg catctgcaat
    19081 accagccacc agcgcctccg gcagcagccc ttgcaggcca gattcactca gttgttggcc
    19141 tacctgtaaa ccaatgccgt agcttgcttg cgcttcgatg gtgtcaaaag ttggggtggt
    19201 catcgtattt cctttcttag ggtataaaag tagcgggcag catatcagcc acacttcttt
    19261 gggtaaaact ttgtcgcagg aatgatgaca aatcccgggg aaaaagaaac aatagcaata
    19321 tcccgtatga atataataaa ggcgggttta ccacggacat catagcgtta gcttattcat
    19381 aggctatgat tgaggaacaa gacgcggagc aggaggaaac catgcccggg cgctttgaac
    19441 taaaaccaac cctggagaaa gtctggcacg cgccggacaa ttttcgcttc atggacccgc
    19501 tgccgccgat gcatcgtcgg gggatcatta ttgccgccat tatattggtg gtcggatttc
    19561 tgcttccatc tgatgatacg cccaacgcgc cggtggtgac gcgtgaagcg cagttagata
    19621 ttcagtcgca atcacaaccg ccgacagaag aacagctgcg ggcgcaatta gtcacaccac
    19681 aaaatgatcc tgatcaggtg gctccggtcg ctccagaacc tatacaggaa ggccagccag
    19741 aagaacagcc gcaaactacc caaacacaac cattccagcc agatagcgga attgataatc
    19801 aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cgcgaccacg
    19861 gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga
    19921 gtaatttgca aaacgggcaa atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg
    19981 gcttaaccat tgatacgggg aataatcaac aggtcctgtt cacccgccag ccggatggca
    20041 gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt
    20101 gatgacctta cgtctggctt ccagtgcctg gggggaaacc atactgatgt gataaaccag
    20161 gccaagcgta tgctggttga tcaaataata tccgccaagc gcagcaatat taatattcac
    20221 ctgcaatggg tcgatatctg gcttaaaaat attgtgtttc ttcccttcat ccaataagga
    20281 ttccattaac tgcaagtgcg catggttaat ttctaagaga cgctgtgatt ttgcataatg
    20341 cacaccttta ctttgatttt cactatgcac aatttttaaa aaccagggat tagcaatata
    20401 ataatcccat gtaaaatcaa taattttctc aatggctttt tgtggcggaa gatctgagaa
    20461 atcaagggcc ttttcttttt gtcgaatatc gctccaggta tattccagta cttcaataaa
    20521 taaattttct ttattaccaa agtgatgata aaccgtctgt ttactacacc ccgcagcctt
    20581 aacaatatta tcgaccctgg ccccttcata accatattcg gcaaattcat taaccgcgca
    20641 taacagcagc ttttccttca ggctctgtac gttgctggta tcaagataca taacgcttcc
    20701 ccacgacctc ttcaagataa ctatcttaac ccgattaatt agaagctcca gtaaaaagga
    20761 taaaaacttg tactttcatc aagttatcaa atgtcctgtg atcactatcg catatcaacc
    20821 aaccagatgg ttgatctttc ttgcgtcacg tcgcaagcta attgtcactt agaaaacaat
    20881 attcaaaaaa agagagctat tgtatgaaat ccactcgtcc tgttgccgtg attaccggag
    20941 ccgctcgtgg tattggaaag ggctgtgcgc tggagcttgc tcgtggcgga tttaacctgc
    21001 tgattaacga tcttcccgat gccgacagcg tagaaaaact gcatatcaca caacaagaat
    21061 gtatagctga aggcgtggaa gtgatttgct tccctgccga tgtcggcgat ctctccttgc
    21121 atgaagagat gctcgacgcg gcgcaaaacc agtgggggcg tctggactgc ctgctcaata
    21181 acgcgggaat ttcagtgaaa aaacgcggtg acctcctcga cctcgaacca gacagcttcg
    21241 atcaaaacat tgccattaac acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc
    21301 gtttactggc acagccgaaa ccggaagcgg aactgccgca ccgctcgatt attttcgtca
    21361 gttccatcaa cgccatcatg ctggcgatga atcgcggcga atacaccatt gccaaaaccg
    21421 ccgtttctgc cgctgctcgt ctgtttgctg ctcgcctttg caatgaacaa attggcgttt
    21481 atgaagtgcg ccctggtctc atcaaaaccg atatgactat tccggcaacg gcttattacg
    21541 acgaactgat tgccaaaggt ctggtgccgt ggggacgttg gggttatccg gctgatatcg
    21601 cttccaccgt ccgcgcgatg gcggaaggta aactgattta cacctgcggt caggcggttg
    21661 ccatcgacgg cggcttaagt atgccgcgct tctgaggctg aatcatgaga gatctgttac
    21721 aacgcccgga tttattcagt atcaataccg caacactggg ctataaaacg ccgctacccg
    21781 ccattattga tgcctgcgcc gcacgcggta ttggcgcaat tgccccctgg cgcagggagt
    21841 tgcagggtga agatctccag caaatcgccc gccagcttgc cgccagtaac atgaacgttt
    21901 ccggtttgtg ccgtagcact tactacaccg cgccgacact ggcggaacgc aaactggcga
    21961 ttgatgataa ccgccgggcg ctggacgacg cagcggtgct taacgccgcc tgttatatgc
    22021 aggtggttgg cgggttaccg cagggaacaa aagatttgta cgaagcgcgg gaacaagtaa
    22081 aacaagggat tcgccagttg ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg
    22141 agccgctgca cccgatgact gctgccgacc gctcttgcct gtgcacgtta cgtcaggcgc
    22201 tggactggtg tgatgaactc gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg
    22261 tttatcacgt ctggtgggat ccggatctcg ccagtcagat cctgcgtgcc ggaaaccgca
    22321 tcctcgcgtt tcatgtttcc gactggttag tgccgaccac cgatctggtc aatgaccggg
    22381 gaatgccggg agatggcgtc atcaatattc cgtcaattcg tcggctggtt gaaaacgccg
    22441 gatttaatgg cgctattgaa ctggaaatat tctcgccgta ctggtggcag aaagatatta
    22501 acagcacgct ggatattagc gtcgatcgca tcgcgcatta ttgttaagga atgatcatga
    22561 gtgaccaacc cgttctgttc agcagagcgg cagcttcctg ccgcctgaca ttgaaccgtg
    22621 aagataaatg tcatgctatt aacgaagaga tgattgaatc tctcgaccat tatttaaatg
    22681 aaattgaaaa cgacaccaca ttacgattag tcgaattaac ggcaaccggc gataaattct
    22741 tttgtgcggg cggcgatatt aaatcgtggt cggcctattc gccattagat atgggcagaa
    22801 aatggattaa acgcggtaat gacgttttta atcgcctgcg taacttaccc caattaaccg
    22861 ttgcgaatct caacggtcat accatcggcg gtggtattga acttgccttg tgctgcgata
    22921 ttcgtattgc tcgccccggc gcgaaattct ctaacccgga agtgatgctc ggcatggtgc
    22981 ctggctggat gggcattgaa cgtgtgctta atcaggttgg tccggtggtc ggtcgccaga
    23041 tgctgatgct gggtaaacgc ctgacggcgc aggaagctca ggcggcgaat ttaattgatg
    23101 aagtggtaga aaaagaacag gtggaaagct ggatggcgaa ccagttagcg cagctggaaa
    23161 aatgtggtcc ggtggcgctt gcacatatta agcaactgat tctggcactg gaaaataaac
    23221 atgccgatta cccgcaccag ctactggccg gattaatgtc cgccactcag gattgccagc
    23281 aggcgacgcg cgcctttgcc gaaaaaagta gcgtgagctt caataatcaa taaggaaatt
    23341 gtatgcgtaa gataacaacc gccgaggcac tggcggcgca aattcaggac ggtgcgacta
    23401 ttgctattag cggtaacggc ggcggtatgg tggaagccga ccatattctg gctgctattg
    23461 aagcgcgttt cctgcaaacc ggacaccctc gcgacctgac gctaatccac tcgctgggta
    23521 ttggcgatcg cgactgcaaa ggcactaatc gttttgcgca cgcagagatg ctcaaacgca
    23581 ttattgccgg gcactttacc tggtcgccca aaatgcaggc gctggtaaaa aataacacga
    23641 ttgaagccta ctgctttcct ggtggcgtga ttcaggcact gctacgggag atcggcgccg
    23701 gacgtccggg gctttttacc cacgttgggc tgggatcgtt tgttgatcca cgcaatggcg
    23761 gcggtaagtc gaatgaatgc actaccgacg agctggtaga actgattgaa atcgacggtg
    23821 aaaccaaact tcgttatcgc cctttcaaag tggattacgc gattttgcgt ggcacttatg
    23881 ccgatcctcg aggcaacgtc agccttgaag aagaagcgat tgatatggat agctattcca
    23941 tggcgctggc agcacacaac agcggcggca aagtgttcgt acaggtacgc gatgtgctgg
    24001 aagctggcgc gattgaacca cgccgggtta aattaccggg cattctggtt gatggcatcg
    24061 ttgaacaccg cgaacagcca caaacctatc ttggcggtta cgacctgacc atcagcggcc
    24121 aacatcgccg tctgagttct aacgacgcta ttgaactggt tagtcatccg gtacgtcgcc
    24181 tgattgcccg tcgggcagca cgcgaactgg tagctggcgc ttcaaccaac tttggctttg
    24241 gtattcccgg cggcattcca ggagtagcgc tgcgcgaagg cgttccttac caaagtttat
    24301 ggctgagcgt cgagcaaggc gtacataacg gcatgatgct ggatgatgcg tttttcggct
    24361 gcgcccgcaa cgccgatgcc attattccat cactcgatca atttgagttc tacagtggcg
    24421 gagggatcga tatcaccttc ctcggcatgg gagagatgga tcagtacggt aacgtcaacg
    24481 tctcccatct caatggcaat ctgattggcc ccggcggatt tctcgaaatt gcgcaaaatg
    24541 cccgtaaagt ggtgttctgc ggcacgttcg acgctaaagg cagcaagatc gatataacac
    24601 cagatggctt gcatatcgcc cagtcaggtc aaatccctaa actggttacc aaggtggaaa
    24661 aaatcacttt tagcgccgcc tacgcacagc aaagtggtca ggaagtgttg tatatcactg
    24721 aacgcgcagt attccagtta acggcagaag gcgttgaatt aattgaaatc gcgccgggtg
    24781 tggagattga gcgcgacatt ctgccgtata tggctttccg tccaattatc aagcacccac
    24841 gcctgatgga aagtagcctg tttacgccga tggaggatgc atgagtcagc ttgatgacac
    24901 catccttgat gcgctgacgc acgttacttt cccaaagggt tttgcacagg cagagcccgc
    24961 atgggttgtc acggtagacg gtgttgatta cccactctgg caaacagatg ccctggtagt
    25021 cggcagtggt gcagccgggc tgcgtgcagc tgtggaactt aaacgccgcc agcaaaatgt
    25081 gctgatcgcc accgccgggt tatatatggg gacgtcggca tgttctggtt ccgataaaca
    25141 gacactgttt accgccgcta ccgcgggcaa cggcgacaac ttcaccaaac tggcagaggc
    25201 actggcgagc ggtggggcga tggatcacga caccgcttat gtcgaagcgg taggttctct
    25261 gcacactctt ggcgggttgc aatatcttgg tctggaatta ccggaagatc gctatggcgc
    25321 gattcttcgt tatcaaaccg accatgacga agccgggcgt gcaacctcgt gtgggccgcg
    25381 tacttcaagg ttaatggtga aagtgctgtt ggaagaagta cagcgcctcg ccattccagt
    25441 gttgaccagt gcaacagtga ttaaactgct gcatcagcgt gacgaaaacg gcgaagaccg
    25501 tgttgcgggg gcaatcctcg cgaccggtca tcgcgcccat aacccttggg ggctggcaat
    25561 tgtgactgcg cccaatgtgg tactggcaac aggagggcct ggcgagcttt atcgcgacag
    25621 tgtgtaccca cacaaatgtt ttggctcgct ggggctggcg ctggaggaag gcctgacgct
    25681 aaccaatctg accgaaagcc agtttggtat aggcacgccg cgtagcacgt ttccgtggaa
    25741 tttatccggc acctatgtac aggtgatccc gtatatctat tccgtggatg ctgagggtaa
    25801 cgagtataac ttcctcgcgg attactatcg caccacccag gagctggctt caaacatttt
    25861 ccgtaaaggc tatcagtggc cgttccacgc cactcgggtg atggattttg gctccagctt
    25921 gttagatatg gcagtagcgc aagagcagca atcagggcgt caggtattta tggatttcaa
    25981 tcgcaatcct gaagcggtgc cgggtgacct gccgttctca ttagatcgac tggacgacga
    26041 cgttcgcgcg tatctggaaa ataacgacgc tctggcacca tcgcccatcg aacggctgca
    26101 acgaatgaat ccgctgtcta tctcgctgta taagatgcac ggttacgatc tcaccacaca
    26161 gcctttgcag tttgccatga ataatcagca tatgaatggc ggcattgaag tggatatctg
    26221 gggacaaacc tccctgcccg gctgtttcgc cgtgggggaa gttgctggca cacacggcgt
    26281 cactcgccct ggtggtgcag cattgaatgc cgggcaggtt tttgctgttc gtctggcacg
    26341 ttttattggt tgcacgcaaa agcggaatat tgatggagat atagcacagc tggtagctcc
    26401 tacactggct tctataagag agatcattac tcaggcgcac gataacggga ccgggatgcc
    26461 gttgtcggtt gtgagagaaa aaattcaggc acgaatgtct gaccatgcgg gatttatttg
    26521 ccatgccgat aaagtccgac gcgccactcg cgatgccctg ctattgagcg aatttgtcca
    26581 acggcatgga ttggctatca aacatgtggg tgaagttgcc gagctgttta tgtggcggca
    26641 tatggcactg acttctgccg ccgtattaac gcaattaacg cattatattg acgcaggtgg
    26701 tggtagtcgg ggagcacgta ttattctcga cagggatgga aattctattc cgcaaacacg
    26761 aaatggtttt tgtgatgcct ggcgctttcg ttcagagcgt actgaggata aaaaagataa
    26821 actacttatt cattattgca acggtatctt tcacgtcaga gaaacacctg ttcgtgagtt
    26881 tcctattatc cgaggtattt ggtttgaaaa aaactggcct ggttttctta atgggacaat
    26941 ttaccagcca caggatgaat gatattgcaa aggttaatca ctatgcgaaa tataattaga
    27001 tctattaatg ctctgcttgc agcattgaac attaccattt tggcgatcat cgtggcatgt
    27061 gtgacgtggc aggtggctgc acgatttata tttactagtc ccagtatttt tacagatgaa
    27121 ctcagccgtt tgctctttat ttgtcttgga ctttttggtg gggcatacac cgcagggcaa
    27181 aaccggcatc tggcgattga cctgctacct atgatgctga aaggaaaagc ccgacgtcat
    27241 ttatttttgt gtattcagat tatcgttatt atcttcgcaa ccatcattat ggtatatggt
    27301 ggtgggctat taacgatgga tacattcgat tcaggtcaga cctctcccgc tttaggctgg
    27361 caaatgggat atatctatat gagcatcccg attagtggtg tacttattat tatatacacc
    27421 atagatatgg ttttaaccga acttaaacaa ccactctaaa gataacaaac aacaaccaac
    27481 catagtagga atggagaaat tatggattac tggttaccga ttattgtgtt atttggtgcc
    27541 ttctttttta tgttggcatt aggtgtacct attgtttatg ccattggttt atcgacactg
    27601 gcatcaattt ccacacaact cgatttcaac tcagcattga gtgtggtttc ccaaaagtta
    27661 gcctccgggt tagatagttt tacgttgttg gcaatcccat tttttatctt gtctggcaac
    27721 attatgaacc atggaggaat agcgcgacga ttaatcaact ttgcccgtat tctcggcggt
    27781 cgtctgccag gctcacttgc ccactgtaat attctcgcca atatgctgtt cggggcaatt
    27841 ctggttcagc cgtggcttcg gcggctgcga tgggcggcgt catgcatccg caacaggtta
    27901 aagagggtta cgacccggca ttcagtactg cggttaacgt tgcctctgcc ccgacgggtt
    27961 tgcttattcc accaagtaat acgttaatcg tctattcact ggtcagtggt ggtacttcta
    28021 ttgccgcact gttcctggcg ggttatgttc cgggaatttt gctgggtctg gcactgatgg
    28081 taatagccgg tattatcgcg gtacgtcgcg gttatccaaa gccagaacgc ccgactttac
    28141 gccaggctgg cgttgcgata tggatggcta tccccagtat tttcctgatt attttaatta
    28201 tgggggtgta ttaagtggta tttttacccc gacagaggcc tccgcaattg cagtaattta
    28261 tacccttttc cttgcactgg ttctttatcg tgaaatctca gttaaagatt tacctaaaat
    28321 atttctggaa tccgttatta ccactgcaat tgtcttgtta ttaattggtt cttcgatggg
    28381 tatgtcatgg gcaatgtcaa acgctgacgt tcccttcctg attctcgatt tgcttaacac
    28441 tatttctgac aacccaataa taatcctgct tattatcaat attatcctgt tgattatcgg
    28501 cacttttatg gatatgacac cggcggtact gatttttacg ccgatatttt taccggttgt
    28561 caccgaactg ggcatggacc cgattcactt tggtattgtc atggtactga acatgtgtat
    28621 cggtatttgt actccgcctg taggcagtgt gttatttgtc ggttgtagtg tatctaaatt
    28681 accgattaat aaaattatca aacctatgct gccattctat gccgtgatgg tattggtgct
    28741 ggcaatggtg acctatattc cgcaaatcag tatggcattg ccaagagcac tgggctactg
    28801 atgcgcattc actggcggca cctgccgcca tatttattag aaaaggaaca tctatgaaaa
    28861 taaaagtatc cgctggcatt atcggtgctg ttcttatgtt atccgcaagc cagtcctggg
    28921 cagtgacatt aaaactgagt cataatcagg ataagtctca tcctgttcat aaagcgatgg
    28981 agttctttgc gaaaaagagc aaagagtact ctaacggtga tattactatt cgtatttatc
    29041 caaatggaac attgggtact caacgagaaa caatggagct gattcgttct ggcgctattc
    29101 ccctggtaaa aaccaatgcg gcagaaatgg aagcatttga aaattcctat aaattattta
    29161 gcctgcctta tttgttccgc gatcgtgatc attattatca ggtcatgcag ggcgatatcg
    29221 ggagaaaaat cctcgactca acgaaaagca aaggttattt cgggctgact ttttatgatg
    29281 gaggcgcccg cagtttctat ggcaataaac cagtactgaa accagacgat ctgaaaggca
    29341 tgaaagtccg tgtccagcca agccctggcg cagttgaaat gatcaaagtc atgggcggta
    29401 acccgacgcc actggattac ggcgagttgt atacagcctt acagcagggt gtggtcgata
    29461 tggcagaaaa cagcgtgatg gcgctgacta ccatgcgtca cggtgaagtg gcaaaatcct
    29521 tcagccttga cgaacacact atggttcccg atgtggttct gatgagcaat gctgcgtttg
    29581 ataaacttag ccaggaaaat caggcagtta tattaaaagc agctaaagaa tcaatgagct
    29641 acatgaaaga cttgtggagc gaggaagaga aacaagaatt tgcaaaactg gataaaatgg
    29701 gcgtgaaagt ctaccaggta gataaagctc cgtttatcga gaaagtacag ccaatgtacg
    29761 caaacttcgc taaggacaac ccagcccttg ccccaatgct ggctgatatt caggcagcta
    29821 agtaattatc ttcgtagggc tgcttattgt ggcccttatc gatatctgca agtgctcgct
    29881 acaaaagcaa aacgccgacc aatggtcggc gtttttacgt ctcgttgaat aacgaattat
    29941 tcagctacta cgtttacgat cactttcgcg aatacttcgc tgtgaacctg gaagctcact
    30001 tcgtgttcgc cagtggtacg cagaacgccg ttcggcagac gaacttcgct cttagccact
    30061 tcaacgccag ctgcagttac agcgtcagcg atgtcgcgag taccgatgga accgaacagt
    30121 ttaccttcgt cgccagcttt agacgcgatg gtaacagttt ccagtgcatt gattttctca
    30181 gcgcgagcat tagcagctgc cagaacttca gccagtttag cttccagttc agcgcgacgt
    30241 gcttcgaaga gttcaatgtt tttcttggta gctggaacag ctttaccctg cggtaccagg
    30301 aagttacgag catagcccgc tttaacgttt acctgatcac ccaggctacc caggtttgct
    30361 actttatcaa gcagaataac ttgcattacc ttatcctctc aaagtcgtat taatggaccg
    30421 tgaccgatta ctgatggcga tcagtgtacg gcagcaggga caggtagcga gcgcgtttga
    30481 tagcgcgagc cagctgacgc tggtattttg cacgggtacc ggtgatacgg cttgggacaa
    30541 tcttaccgct ttcggtgatg tagtttttca gcgtagcgat atctttatag tcgatctctt
    30601 gaacgccttc cgcggtgaaa cggcagaact tgcgacgacg gaaataacgt gccatatggc
    30661 tagtctccag aatctatcaa ttcaatctgc tcggcatgca aaaccatttt gctcagtccg
    30721 ttctttgcct tgtggcatga aatgaacccc tgaacggtta tgcgactgcc gaccgttata
    30781 ctgtgagtaa tggcctggtt ttcgtgtccg ctaacaataa cgggcatttg gcaccacgcc
    30841 tgccggtgaa aaccggcttc ctcctgcaca gaacgatgct caagcacgaa ctggcagtga
    30901 ggaattcctg atggactgac ctttcgaagg ggagtcctgc acacggtgcc ggacaacacc
    30961 agacggttgg tcatcagaaa ttactcttca gaatccccag catctgcatc atctgcggtt
    31021 tcgtttgcga aatcatcgcg acgctcgcgg cgctcgtctt tcgctttaac catcggagat
    31081 gcttcggtaa cagcgtgctt ggtacgcata accatgctgc gaataacggc atcgttgaag
    31141 cggaaggtag tttccagctc atcgatcact tcctgcggag cttcaacgtt catcagaacg
    31201 tagtgtgctt tgtgcagttt gttgatcggg taagccagct gacggcggcc ccagtcttcc
    31261 agacggtgga tcttgccttc tgcaccagtg atggcagcag tgtagcgctc gatcatgccc
    31321 ggaacctgtt cgctctggtc aggatggacc ataaaaacga tttcgtaatg acgcatcgaa
    31381 ttgctcctta cggattattc agcctcctgt ctgggtcagc cgaatcccgg ggaggcaagg
    31441 aacgtgttaa aggtcggctg aaaaatgacg cgttatagta ctgacatacc ttgctaaact
    31501 caagggcaga gcagaaataa atctcccctt tcgcaaataa ccactaagcc gctgatttga
    31561 agttcatcac acttcatcat gctgtttttt tgaacaactg catcagaaat ggtcgccatc
    31621 aactttttca tccgtgatta aaattaaatc agaccatcac cacatgagga gagcacacaa
    31681 aaacagccgt cgaggaggta tctatgttca gccgtgtttt agcccttctg gctgtgcttt
    31741 tgctaagtgc aaatacatgg gcagccattg aaattaataa ccaccaggcc agaaatatgg
    31801 acgatgtgca aagcttaggc gtgatttata tcaatcataa tttcgccact gaaagcgaag
    31861 cgcgtcaggc attaaatgaa gagacagatg cgcagggcgc aacgtactac cacgtgattc
    31921 tgatgcggga accggggagt aacggcaata tgcacgccag cgcggatatt tatcgctagc
    31981 accaggtata accaaagaaa cattgccata gtttgctttg cccccttctc agggggcttt
    32041 ttttgaaaaa cggctgcgga attagaccag ttatctcccg aggaaggaaa tttccgcagc
    32101 gcgtgttcta cttctgcccg taataagcgt taggaccgtg tttacgcatg aagtgtttat
    32161 tcatcaggaa gctgtcgatg tgattgagtt gtggattaat actgcgcgca atccacgcca
    32221 ttttcgccac ttcttccatc actaccgcgt tatgcaccgc gtcgtgggca tctttccccc
    32281 aggcgaacgg tccgtgctga tacaccacaa ttcccggcgt atgcagcggc tcggcgttgc
    32341 ccagcgtttc gataatcact ttgccagtgt tcagttcgta ctcaccctgc acctcttctt
    32401 cgcttaatcc gcgcgtgcac gggatatcac caaagaagta gtcagcgtgc gtggtgccta
    32461 acgccgggat cgccagcccc gcctgcgccc atgcggtggc atgagtggag tgggtatgga
    32521 caatgccgcc aagcgacggg taacgacggt agagttcgag atgcgtcgcg gtgtcggaag
    32581 atgggcgata ctccccttcc accaccttgc cgctcatatc aaccaccacc atatcggccg
    32641 ctttcatggt ttcgtaggca acgccgctgg gcttgatcac caccagcccg cgttcgcggt
    32701 cgatagcgct gacgttgccc caggtaaagg tcaccagccc gtagcgcggc agctccatgt
    32761 tggcttcaaa tacctgctgt tttagctttt gcattatgcc gcctccacca tgcccgcttt
    32821 cgccatgcgc gctttcaccc aatcacgcgc tttcgccact tctgccgccg ggtcttccgc
    32881 cgtttcgctc cacatctcaa tcaggtacgg cccgcaatag ccactctgtt tgagcgtttc
    32941 gaaacaacgt tcgaaatcca ccacgccttc gccaaacggc acgtttttga agacgcccgg
    33001 tttggtgtct ttcacatgta ccgcgacgat atgcccgatt ccggcctgca actccatctg
    33061 cacgtcgttg tcccacgccg agaggttgcc gatatccggg tagagctgga accacgggtt
    33121 gttgagatag tgcgcgtagc ccagcgcctt gctgatggag ttcatcaatg ggtaatccat
    33181 gatctccatc gccagcgtca cctgcgcgcg gcttgccatc tcaacgctct ctttcaggcc
    33241 gtcacggaaa cgacgacgcg tttcgttatt ggcttcctga tagtaaacgt catagcccgc
    33301 cagttggatc acgcgaatac cgacatcctg ggcgaactgg atagctttac gcataatctc
    33361 cagcccctgc gcccgcaccg cgtcatcttc actgcccagc gggaaacgac gatgagcaga
    33421 aaggcacatg gacggcacgc gcacgccggt ttcaacaatc gcgttcacca gcgccagacg
    33481 ctgctcgcgg ctccagtcga ggcgcgacag gcgctcgtcc gtttcatcta ccgacatctc
    33541 gacaaaatcg aagcccaacg tttttgccag ttgcaggcgt tccagccagc actccccggc
    33601 ggggagcgct ttttcataga tgccaagcgg gatttgtttg gacaacatat ccgctcctta
    33661 gccccacagt tcggcgatgg aacgtttgaa ctgacgtgca gcttcaaccg gagaagcggc
    33721 atcacggatg ctgcgaccag cgataaacac atgaatcgga atacctttga acagcggcag
    33781 atcttccagc gccaggccac cggtgacggt gactttgaag cccatatcgg aaagacgttt
    33841 gatcgcggta atgtccgctt cgccccacgc tacgcctgcg gcctgcgcgt cacggctgcg
    33901 gtgataaacc acctgctgaa tgcctgcgtc acgccactgc tgcgcctgtt cccaggtcca
    33961 gtagccggtc agttcgatct gcacgtcgcc gttaaactct tttgctacgt ccagcgcgcc
    34021 tttggcggtg ttgatatccg cacagcaaat taccgtcacc cagtcagcgt tggcttcgaa
    34081 gcacatacga gagaggattt tgcccgcatc ggcaattttg gcgtctgcca gtacgatttt
    34141 gtgcgggtaa agcgctttca gatcgcgaac cgcacgcacg ccttcgccca cgcacagaat
    34201 ggtacccact tcgataatgt cgacttcttc ggcaatcagg cgagtggttt cgtaggcgct
    34261 atccatagtc tggttgtcca gcgcgacttg taacatcggt aatgacatct tcaattcctt
    34321 cttaagctgc cgcgttggtg cggtcaatga gatccagtac ttcctgctcg gtacggcagg
    34381 cacgtaaacg gtcgaaattt tcttcatctt caaacaggtt gacgatctgc atgatgccca
    34441 cttcctggtg cgtgttggca tcgaccgccg ccatggtgat gaggatatcc accgggtcgt
    34501 tatcttcgtg gttgaactcc agaggctttt tcagcgtcac cagcgcaaaa ccggttttct
    34561 taacgccctc ttccgggcga ccgtgcggca ttgccaggcc tggtgcgagc acaaagtacg
    34621 gaccgtgctg ttcaaccgca tccagaatcg cctggtagta acgcggctcg acaacatccg
    34681 ccgccaccag caggtcaacg ccgattttta ctgcgtcctg ccatgtctca gcttcagcct
    34741 gcaggcggat ggatttattc tccgccagcg aatcacgtaa tttcatggcg cgtccttact
    34801 tcacatcctg cgggaaatgc tctttgatca cttccagcag tttcgggcca aagtcggcag
    34861 gagagagcat gttgcgcaca ccaaccacgt atttgttgcc ggtcacggtg atttcgcccg
    34921 caatgtgcgt agaagcgatg atgatatccg cgccgctcaa ctcgcttttg tattcgccaa
    34981 ccgcgcagct gtttaccgtg tggtcaatgt ttgattgggt taaaaactgg tccactttca
    35041 tcttcatgat catggaactg ccttgtccgt tgccacacac agccagaata cgtacggtca
    35101 taatcaaaac tccttattaa gcagactgtt ctgccagttg tttttctgca tcttcttctg
    35161 cgcgcagtgc gcggccagcg aagaacatat aagccagtgc aattacaatg atgacggcca
    35221 taaaggcgat accgatggag aagaagccct gcatcatcgg cggtgccaga atcgaccagt
    35281 ccgccatacc catccaggca ctcataccgg taagtttcac cgcccagacg caaccaaaga
    35341 tttcgatcat ccccatcacc agacaaatct tcagcgccgc acgccagccg ccgaagtggt
    35401 tagcgaacac gccgatggtg gcgttggaga agaacatcgg gataaagcta ggaataatca
    35461 ggatcgagga accgcaggcg accaggatgc caaccgcaat cagctgaccg atagtgcccc
    35521 acataaagcc ccagaccacg gcgttcggcg cgaagctata aatagccgca cagtcaatcg
    35581 ccagaaccgc acctgggatc aggcgctggg aaatgccgtt aaatgcttca gagagttccg
    35641 ccacaaacat gcgcacgccc tgagtgatga tgaagatcgc caccgcaaag gagaaaccag
    35701 tttgcaggat gtacaccgtc cagttcactt tgcctgccat cgcctgcacg gtgtcgatac
    35761 cgaaggagag tagaatggca ccaaagaaga tggtcatgac aatcgccgtg gagacgatgt
    35821 tgtcgtggaa aatattcagc cagcctggca gtttgaggtc ttcaacgctt tcctcttttt
    35881 tgccgaggaa cggcgcaact ttataggcaa tccatgatgc aaactgctgc tggtgaccga
    35941 tggagaaacc gcagccgtcc gtcacttcct gagtcggctt gtacatcatg ttggaggtaa
    36001 tgccccagta gagcgaaacc agaatcgccg tgcagataat ggttgtccac atggagtagc
    36061 cgaaaataaa caacgtaacg gcaatcagcc ccgcctgctg gaacatgata tgaccagtca
    36121 gcatgatggt acgaataccg gtaatacgac gcagcagcac gtaacagatg ttcagcgcca
    36181 gcgctaacaa caccgcgtaa cctacccagc tataggcatc gcccatgcgg tcgatggttg
    36241 ccatcatcga cgcgtaagta tcggaaattg cgccgttaat gccgtagact tcggacattt
    36301 tcgccaccac cggtttgaag gtgctggtga ggatgccgga ccctgcctgc aacaacatga
    36361 aaccaattat ggttttaatc gtgcctttaa taataacgct gacacttttg cgcagtagga
    36421 tgtagcccag acaggtcaca atacccagca acaacggggc attggtcatg acctggttaa
    36481 aaaacacggt aaagatgttg tagaggatct ccataacgat ctcctgaaga agaggtaacc
    36541 gggtattccg cacacccgga atgttgtgca ctcactctaa ttttcaaaag taatcacaac
    36601 aagattattt atgattaaat gtgacgcgac ccgcaaataa ttccacacgg atagagtggg
    36661 attcacacaa cgaaacaatt atttatttaa attcatttag ttaatttaac tacacattcc
    36721 attaaatctt tccacactca aaatcaacaa caacttccct ggcaaaaagt caaaaaatgg
    36781 tcattgcctt cctgaaaaaa tagtgtcagg attaatcaaa accaatcaca tattgattcg
    36841 aattgaacta tcatgaaggt aaatggcgat gagtaaagtg aaaagtatca cccgtgaatc
    36901 ctggatcctg agcactttcc cggagtgggg tagctggttg aatgaagaaa ttgaacaaga
    36961 acaggtcgct cctggcacat ttgcgatgtg gtggcttggc tgcaccggga tctggttaaa
    37021 atcggaaggt ggcaccaacg tttgcgttga tttctggtgc ggcactggca aacaaagtca
    37081 cggtaacccg ttaatgaaac agggtcacca aatgcagcgc atggctggcg tgaaaaaact
    37141 gcagccaaac ctgcgcacca ccccgtttgt tcttgatccg tttgcgattc gccaggtcga
    37201 cgcggtactg gcgactcacg atcacaacga tcatatcgac gtcaacgtcg ctgctgctgt
    37261 gatgcagaac tgtgctgatg acgtaccgtt cattggaccg aaaacctgtg tggatttgtg
    37321 gattggctgg ggcgtaccga aagagcgttg catcgtggtc aaaccgggcg atgttgtaaa
    37381 agtgaaagac attgaaattc atgcgcttga cgctttcgac cgtactgcac tgatcaccct
    37441 gcctgccgat caaaaagcgg ctggcgtact gccggacggc atggacgatc gcgcggtgaa
    37501 ctatctgttc aaaacgcctg gcggctccct gtatcacagc ggcgactccc actactctaa
    37561 ctattatgcg aagcacggta acgaacatca gatcgacgtg gctttaggtt catacggcga
    37621 aaacccgcgc ggtatcaccg acaaaatgac cagcgccgat atgctgcgta tgggtgaagc
    37681 cctgaatgcg aaagtggtta tcccgttcca ccacgatatc tggtcaaact tccaggccga
    37741 tccgcaagag atccgcgtgc tgtgggagat gaaaaaagat cgcctgaagt atggcttcaa
    37801 gccgtttatc tggcaggttg gcggcaaatt tacctggccg ttggataaag acaacttcga
    37861 gtaccactat ccgcgtggtt tcgatgattg cttcactatt gaaccggatc tgccgttcaa
    37921 gtcattcctg taatctgtcg tttataccgg atagcgtgga gtagttcgcg ctatccggcg
    37981 tagtatttcc tggctatttc aaatatcatc taaaaaaatc aaattttatc ggaatagctc
    38041 atgactgaag cacaaagaca tcaaatcctc ctggaaatgc tcgcacaatt gggctttgtg
    38101 accgttgaga aagtcgttga gcgtctggga atttcgcctg ccactgcgcg acgcgatatc
    38161 aataaacttg acgaaagcgg caaactgaaa aaagtgcgca atggcgcaga agctattacc
    38221 caacagcgcc cgcgctggac gccgatgaat ctgcatcagg cgcagaatca cgatgaaaaa
    38281 gtacgtatcg ctaaagcggc ctcgcagctg gttaatccgg gcgaaagcgt agtcatcaac
    38341 tgcggctcca ccgcgtttct gcttgggcgg gaaatgtgtg gcaagccagt gcaaatcatc
    38401 actaattatc taccgctggc aaattacctg atcgatcaag aacatgacag cgtgatcatt
    38461 atgggcggac agtacaacaa aagtcagtcc atcactttaa gcccgcaggg cagcgaaaac
    38521 agtctctatg ccgggcactg gatgtttacc agcggcaaag gactgacagc agaagggttg
    38581 tataaaaccg atatgctgac ggcaatggca gagcagaaga tgctgagcgt ggtagggaaa
    38641 ctggtggtac tggttgatag cagtaagatt ggcgaacgcg cgggaatgct ttttagccgt
    38701 gccgatcaaa ttgatatgct gatcaccggc aaaaatgcta acccggaaat cctgcaacaa
    38761 ctagaagcgc aaggggtcag cattctgcgt gtttaaagat gctggcggaa aaatgtcact
    38821 gcagcatcta acgcctcagg cgtaatgcgg tgacgcacgc ctggctgcca tgaacaggtt
    38881 agcagtttat cccgtcccgt ctcgcttaag gcctgctgca aacgtagtga ttcgtcggca
    38941 ggcacaacgt catcgtcgag gccatgccac agcagtagag gtctgtcact aagttgttcc
    39001 aggtggtttg tcgcttccca ctctgccagc ggcgcgacaa tgttattgaa ttcattctgc
    39061 tgtgctgtcg tttcaggtat cagcggtgga aacagtgaac gggcgagtga tgtaaaatag
    39121 cccgatccca tcatgctggc ggtacatctc accgtggggt ggcgagcggt aatccccagt
    39181 gccgtcatcg cgcccatcga cgcaccaccg actgccagac ggtcatcaag cagccacttt
    39241 tcttcggcta ttgccgcacg taaagtagtg aattcctgca tactttgtag caagatttgc
    39301 cagaattgat ttaaccgccg cgctgcgtcg ccactaaaac gactaccgtg atcgggcgca
    39361 tccggcatga tcacccgcaa accagcctgc gccagcgcaa cggcaaaata gctatacacc
    39421 agactggacg aagtaaagcc gtgataaaaa atcgcgcacg gtaacggggt atctttttgt
    39481 ccgacaggat aagcatgaag aacgggaata tctgccagct cgcgtgattc tatttcaatc
    39541 atctgtctct cctttttgtc tggtatgaca tgaagaaatc cttagcacaa gtttctgctg
    39601 gcggtattag ttcataatgt tgagatgtgg gttacgcttt cgtcacgttt tcattcgaaa
    39661 atcgcgtacc aggtaacaat tcgggaacat tcccccaaag ttaaattaat aaggcactac
    39721 actatggtta gcaggaaacg taatagcgtt atttaccggt ttgccagttt attattggtg
    39781 ttgatgttaa gtgcctgtag cgcactgcaa ggtacgccac agccagcacc gccagtaacg
    39841 gaccatccgc aagagattcg ccgcgaccag acgcaagggt tacagcgaat aggtagcgta
    39901 agcacgatgg ttcggggttc tccggatgac gcattagcag aaatcaaagc gaaagctgtc
    39961 gctgcaaaag ctgattatta cgttgtcgtc atggtggacg aaaccatcgt gacaggacag
    40021 tggtattcac aagccatttt gtaccgtaaa taaatcagac aacttacata gtcagcttta
    40081 cattgctttg cgtccctatg cgtttacctg tgcacaataa attaccggct ggaagcctgg
    40141 gcgtttatta ccgcgatgga atgccctgca acatgtgggg aaacgaaaaa tggagctgac
    40201 gatgaaacaa ttacttgcct caccctcgct gcaattagtg acttatcctg cgagcgccac
    40261 ggcacagtct gcagaattcg ccagtgctga ctgcgtgacg ggcctgaatg aaataggtca
    40321 gatctcagtt agcaatattt caggggatcc gcaggacgtg gaacgtatcg tggcgttaaa
    40381 ggccgatgaa caaggtgcgt catggtaccg cattattacc atgtacgaag accagcagcc
    40441 tgataactgg cgcgtacagg cgatactgta tgcatgagtg tcatctacag caaatgaaca
    40501 atgccggatg cggcgcgagc gccttgtccg gcctacaatt ttcacatatt tcatttagtt
    40561 aatcgaaacc agcgtcgcat cagtcgtata cgcttacaca cacactcccc ccgtcgcccg
    40621 cagcaataaa tcattctgga tctgctctga caaccgtacg ccgccgcgcg tatctaacat
    40681 cacctgacac cacgcctgcg ccattggcgg agaagcatat ttcaacattt gcgcaccgca
    40741 gcccagcagg aatagctgat gagtaatctc tcgccccagt tcttcagctg gcttacgcag
    40801 ctgctgctgt aaacgacgaa ccgcgcgatc aaaatagcga tcctgccctt tcacttccac
    40861 aaatgcttcc gacaataagt cgtatacgcc cgcttgctta ttgagaacgc gcaacacatc
    40921 caggcacata atattgccgg aaccttccca aatactgttt accggcatct cccggtaaag
    40981 ccgcggcagc tcgctctcct cgcaataacc aatgccgccc agcacctcca tcgcttcggc
    41041 cacaaacggc atacctcttt tgcagatcac aaattttgcc gcaggcgtaa ataaacgcgc
    41101 ccacagggct tctttggcat cggcacgccg gtcccacgct cgcgcaagac gaaacagcaa
    41161 cgccgtttgc ccttcaagct gaagtgccat gcgacttaag acatgacgca taaggggctg
    41221 ttggatcaat ggattaccaa aaacatggcg ttgatgtgca tgataaatcg ccagcgaaaa
    41281 tgcacggcgc atcatggcat ggctacccag ggcgcaatca aaacgcgtca tcccacccat
    41341 tttcaggatc agacgaattc cttccccttc ctgccccaac aaccaaccaa tggcatcctg
    41401 aaactccact tcgcaactgg cgttagagcg attacccagc ttatctttca gccgctcgag
    41461 gcgaatcgcg ttgcgttgcc cgtcaggcaa aaagcgcggc acaaaaaagc aggacagacc
    41521 acccgcggtc tgcgccagca ccagatgcgc atcgctttgc ggaaccgaga aaaaccattt
    41581 atgccccacc agccgataag agccatcttc cagacgctct gcacgggtgg tgttgctcat
    41641 aacatcggaa ccgccctgct tttccgtcat tcccatgcca atcaacaaac cgcgtttttg
    41701 cccacctggc aataagtgag aatcgtagcg atcgctcaat agcggcgtgg tccagtcctg
    41761 aaacggcgcg ggtaacatct gtaacaacaa tggcgtggcg gcaaaggtca tggttatcgg
    41821 acataacgac cctgcctcaa cctgcgcatg taacataaaa cgcgccgcgc gcgccacaaa
    41881 tgcgccggag cgagcgtctt cttcccaggc aagattgtgc acccgattgg tacatagcgc
    41941 ctgcatcagc aggtgccagg cggggtgaaa acgcacatcg tccagacggc gtccttgcgc
    42001 atcgtagcgc aataattcag gcggattcac attcgccagc cgccccagtt caagggattc
    42061 agccgttcct aactgctgac caatactggc tagaaaatcg ctatcccagc cagcaccttc
    42121 acgcgttacc gcttcgcaga gcgcgccatc agacaggtat aagttgctgt tatttaatgg
    42181 tataggttga ttaaaaacgg tgtgagtttg ccagtgcact gtgtctccct ccatcaatgg
    42241 cagtcaccat tagtatggtc actgccatgg attcatgaca gaaagagatt gctaaaacat
    42301 tcttatccag caatgtaatg cggaacgaaa cgtgaggtgt ctttggtgat cagtgtgtta
    42361 tcttcacgga tccccattcc gcacgcttca tcatcgacaa tccagctacc gatgagtgtg
    42421 tagctatcgc caaaccgcgg cagaggttga aacgcctggt agatcatcgg ttcatcggcg
    42481 taatcaccat cagcgtggtc aacgacgtta ttctggccgt cgaaaatggt gacatttccg
    42541 ccttcgcgcg agtagagcgg tttacgcaca tagctttcgc cagcggcaat ctgcgatttt
    42601 tcaccctcaa accaggatgc aagaaggtta ggatgaccag ggaagaagcg ccaaagcagc
    42661 ggcattagcc ctttattact caagatactt ttccataacg gctccaccca ttgctcgcga
    42721 cgcttgcgca gcagcggacc gttatcgtca cgcatcatcc actccagcgg atacagctta
    42781 aatgcacgct ggatgacatt atcatcaaga tcggtcagta cgccgccgac gcccaaaccg
    42841 agatcttcaa tgtagataaa ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag
    42901 tacagcacgg tactcctgtc ttcatcggtg tcctgacagc agcaaaaata aaacggttcc
    42961 cgactgtaaa gctcgctaaa gcgtgaaatc aggcgttcct gaatagcatt gtactgatcg
    43021 gcatcacgcg gaataatgcc gctgcgccgg gcatcctcca gccacagcca ctggaaataa
    43081 gccgactcgt acaatgaagt tggcgtatcg gcgttgtact ccagcagctt caccggcgca
    43141 ttgccacacc aggcaaaatc catccgtcca tacagcgaag gatcgcgggc gcgccagctt
    43201 tcagcgatca catcccagta caacggcggg atcgccagtt gcgtcaggat ctcttcgtct
    43261 ttaaccgcgc gatccaccac ctcaaggcac atctgatgca gttccgcagt cggtttttcg
    43321 atctgctctt caatctggcg cagagtaaaa cggtaagccc gactctcgtc ccaatagatt
    43381 tcattgtcga tgatatgaaa gtcgaaaccg ttgtcagcgg cgatttggtc cagatcccgc
    43441 cgcacaggaa cgttgtgtct cagcatgatt agcctcccca atgcccacgg gcgctggaag
    43501 aacgaccata gccgccgcgc gaaacggtcg acgcttttct ggtggtaaag ccgcctgaag
    43561 agtaagactc ttttttgcca gaaccggagc gccaggagta atcaccagaa gtgttgcgcc
    43621 agacggggcg cgaagcaaaa gaggaaccgc cgctgttata aacaaactgc tcatcgcgat
    43681 ctttacgaat aacccggctt aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa
    43741 cgttgtcgta gtaacaattt tcatacttag actgacaatt ctgctgagtc atattcttcg
    43801 gaacatcggc ataaaatgct gctttggcgt tgttccagcc acgcgcgcaa atgtcggcat
    43861 tattaccgtc atcaatacaa tcctgcacag tcgaataaaa cgttccgtcg ccgtcgttat
    43921 cgacatcgcc actatcgcca caacctttca atacaaaaaa agcggcaccg cccattagcg
    43981 ccaacgttaa gtatttttgt gcgtagcgat tgcgacgcgg ttcaaaagga ttattcggtc
    44041 taccaatgcg actgatcgct ccgtgaccaa ttttactgtt atttcttgat ttgcgttttc
    44101 tggccatgat cgtccattac catgtcatac aagctgcgtt aaaaataccg ccagccagcg
    44161 cggcggttcc cataaacatt cctgctgcgg tattgtgatt aataattttt tcgcttaatg
    44221 cgggcatata aagtctgaca ccagcaaaaa cgagtaactg aatcaccagt gcaatcccgc
    44281 cccaggcgaa atagtctgga atactcaccg cattgattgc cgcactggat aaggggatca
    44341 cgtaacccaa caatgtaccg ctgaatgcca gtgacgccgc ggtattattg tttttgatta
    44401 attgccattc gttgtgcggt gtaattttgg agtagataaa cagaaaaata atcaccatag
    44461 ccacgccaat aaaaaaatag gcactaaagg caagaagtga atccagtata tgcattgtaa
    44521 acgtccttgt aattaaccaa taatatgaag tgatgtcagc gggatatcga cacccaacgc
    44581 gcgcgaaaat aaccactctg gctcgccgag atcgttaaaa gattcctcac catttagcag
    44641 caaatattcg taagtatctt cggtcacttg ccgctggtag cccatggtaa aattatggac
    44701 ttcccattta gcatgatttt gattttctac tttttccagc atgtagacgg gttcgatatt
    44761 tcccggttct tcgctgttaa aaaatcgctg ccagcgtttt tcctgccagt ttaaggtcat
    44821 tgcccccatc gttttggcgt tgatcgcctc gcgccagtga ctctctttac tgataccgta
    44881 gctctcttca tagacaaaga gcttgatatc atcaatgtca tcaatatctt cgccgccggt
    44941 ggtattgatt tgcagaaact catcgcctga agtgtagtag cggaaaatct gactaccgcc
    45001 gcccagagcg atgtggctga cagcagctac cgtaaattcc tcacccggca gcgcaatcag
    45061 caattcatct tccagcaaac gaaacgctaa cgtgtcgagc gtaaagccac tattgagatg
    45121 aagtcccagc ggaccgcgag cgatagcagg cttattatcc ttgccaaaca gacgctggaa
    45181 aaaaccagac atattttatc cttaaaatag ttgccagcct tttagcgagg ctggcaaaaa
    45241 aattactcgc cctgttggcg ttgcagtctt gctaatacat cctgggcgct acttttattg
    45301 ctaccgccaa ttccggcttc cgccagcttt tcgtcaaggt cgcgaccgtc tgcgactttc
    45361 tccaactgtg cggcagcatc caggcgagcc tgacgttcgg cctgacgcgt ttgcaggcgt
    45421 tttaaggatt ctgccgctgt cgaaacgctg gaagatgcgc caacggtaga ggttgttaca
    45481 gcctgttgtg cacgctgcat ggcttcagtg gctttaacga cttccatttg ctgctcaaac
    45541 tgagcgatac gctgcgctgt cgctgtaact gctttttcca cgccatcacg ggagacttcc
    45601 agattcgaca acacttgctc ttcagtggta atgagattct caaggcgcgc gatttcttca
    45661 gcaacttcgt taatcaacga cggattaacg ttcttgctta acgcttccag cgcacgggct
    45721 tccagactgg ctttgcgctc acgtaaatct ttcagcttat cgtgacttaa tttcacccgc
    45781 gccagcagat caacgcgaga ttttccggct ttatcgagtt cagctttagc gtcacgaata
    45841 tgctgttcca gcatgcgcac accctgggtt tcttcaatgg actcttccgc ctgggagata
    45901 aacgatttcc ccagcgtaaa taaacttttt aaaattccca taaatacccg tccttgtaat
    45961 tagtgtgaat attcttctgt aatttcagcc agatccaatg cgttatctac cagcgaggtt
    46021 atctcaagca ggatatcttc aagagaagat ttaagcgata acgccccgaa aacaatgtaa
    46081 tactcttcct gttgtacgct ggagatccct accgatgaca acggcatcat cttctgattt
    46141 cttaataaga aggtattaaa ttcatctgga ttgctgatac tactcaccgg acaaataaag
    46201 gtttcgataa tcatttggcg ggaagtaaaa agaatattga tttgcaaatc gccataatca
    46261 ttcattttaa taattaatgc gttctcgcta tttgttacat caatattttg ttcaggtaca
    46321 gtttgcagcg ccgtcgctag cgccaacgga ttccatgtca tatattattc catatagatt
    46381 aagtttaaat attaataaaa tgaatatttg caatacgtaa ttatcttacc agctatagac
    46441 aaaaaaaaac catccaaatc tggatggctt ttcataattc tgagaaatta gctgcgctgg
    46501 cgcaccgctt caaataagca aattccggtc gcaaccgaaa cgttcaggga agaaacgctt
    46561 ccagccatcg ggatgctgat caactcatcg caatgttcac gagtcaggcg acgcatacct
    46621 tcaccttccg cacccatcac cagcgccagg cgtccggtca ttttgctctg atagagtgta
    46681 tgatccgcct cgcctgccgt accgacgatc cagatattct cttcctgcaa catacgcatg
    46741 gtgcgcgcaa ggttagtcac ccgaatcagc ggaacgcttt ctgccgcgcc gcaggctact
    46801 tttttcgccg tagcgttgag ctgtgcggag cgatctttcg gcacaatcac cgcatgaacg
    46861 cctgcggcgt ccgcactacg caggcacgcg ccgaggttgt gcggatcggt tacgccgtcg
    46921 aggatcagca ggaacggttg atcgagcgaa gcgatcagat ccggcagatc gttttcctga
    46981 tactgacgtc ctggcttcac gcgggcgata atgccctgat gcacggcacc gtcgcttttc
    47041 tcgtcgagat attggcggtt tgccaactgg ataaccacgc cctgagactc aagggcgtga
    47101 atcagcggta acagacgttt atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt
    47161 tcaggggcgc gctccagcag ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt
    47221 aatgtactcg ttgtttacgt tttgcctgat gcactacgtt tatccggcct acatgatctc
    47281 tgcaatatat tgaatttgcg tgattatgta ggccggataa ggcgttcacg ccgcatccgg
    47341 catgaacaaa gcgcattttg tcagcaatct aaccctcttc ttttaaagag ggtattgatc
    47401 actctgccac ttttttcttc gccgcacgct tcgctttggt cgctgcagct attttctgcg
    47461 ttttcgccga tggctttttc gcttttctcg cgtctttctt cgccgctttc ggcttcgttt
    47521 ttttctcacc gcggaaggcg ctgtctggct caaagtttac ctttttaccg acctgacgac
    47581 gcttgccgcc ttttttacct gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga
    47641 cgttgcgcgg tgcgcgttcg ctggagatca ggctaaagtc gattttgcgc tcgtccatat
    47701 taaccgcttc gacgcgaact tccacgcgat cgcccaggcg ataagtctgg ccgctggatt
    47761 cccccatcag gcgttgccct acctggtcaa agcgatagta gtcattgtcc agcgaagaga
    47821 catggaccag accatcaatg aacaagtcgt ccagacggac gaagaagcca aagccagtga
    47881 cgctggaaat tacgccttta aagacgttac ctacctggtc gagcatgaag tcacacttca
    47941 gccagtcagc cacatcgcgc gttgcttcgt cggcacgacg ttccgccatc gaacagtgct
    48001 gacccagttg cagcatctct tccatcgaat aatggtagcc gccggtttca gtggtgttgc
    48061 cctgatgccc ctgctctttc gccagcagat atttaatggc gcggtgcagc gtcaggtctg
    48121 gataacgacg aatcggcgaa gtaaagtgcg cataggactg caatgccagg ccaaagtgac
    48181 cacggttttc tggatcgtaa atcgcctgtt tcatcgagcg cagcagcatg gtttgcagca
    48241 tttctgcatc aggacgatcg gcaaccgact ccagcagctc cgcgtagtca cgcggttccg
    48301 gcttgttacc gcccggcagt tccagcccca gctccgccag cactgaacgg aaagaggtaa
    48361 tcgcttcggt gctcggcttg tcgtgaatac ggaacagtgc cggttctttc gctttctcaa
    48421 cgaaacgcgc cgccgagata ttcgccagaa tcatgcactc ttcaattaat ttgtgcgcgt
    48481 cgttacgctg ggtctgttcg atacgttcaa tacggcgttc agcgttgaaa atgaacttcg
    48541 cttcttcgct ctcaaatgag atcccaccgc gttcttcacg ggctttatcc agcactttat
    48601 agaggttatg caactcttcg agatgcttaa ccagcggggc gtactgctcg cgcagatcct
    48661 gatcgccctg cagaatatgc cagactttgg tgtaggtcag acgcgcgtga gagctcatca
    48721 ccgcttcgta gaatttgtag cccgtcaggc ggcctttcga cgaaaccgtc atctcgcaca
    48781 ccatacacag gcggtctacc tgcgggttga gcgaacacag gccgttagag agcacttccg
    48841 gcagcatcgg gataacctgc gaagggaagt acaccgacgt gccacggtta cgcgcttctc
    48901 tgtccagcgg cgttggcgga cgcacatagt agctgacgtc ggcaatcgcg acccataaac
    48961 gccagccgcc gccgcgtttt ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt
    49021 cgccatcaat ggtgaccagc ggtaaatcgc gcagatcaac acggcccgct tttgcttctt
    49081 ccggcacttc ttctttcagc ccggcaacct gttgctcaac agcctgcggc cagatgtacg
    49141 gaatttcatg ggtacgcaga gcgatatcaa ccgccatgcc ggtgcccata ttgtcgccca
    49201 gcacttcgac gattttaccc accgctttgg tgcggcgagt cggacgctga gtcagttcga
    49261 ctacgaccac aaagcccatc cgcgcgccca tgatctgatc gggcgggatt aagatatcga
    49321 agctcagacg gctatcgtca ggaaccacaa agccgacgcc cgcttcagta aagtagcgac
    49381 caacaatctg gctggttttt ggcaccagta cgcggacaat acgcgcttca cgacgacctt
    49441 tacggtcagc gcccagcggc tgcgccagca cctgatcgcc atgaatgcag gttttcatct
    49501 gctcgctgga gagatacaaa tcatctttac gcccttcaac ccgcagaaag ccgtagccat
    49561 cacggtggcc aataacggta cctttcacca ggtcgaggcg ttccggcagc gcatagcact
    49621 gacgacgagt gaagaccagt tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc
    49681 cttcaagctg ctcttcgcct tcaatgtgca gttctaccgc cagctcatca cggctggccg
    49741 gtttttcacg tttggttaaa tgttcgagga taaattcccg actagggatg ggattcgcgt
    49801 atttttcagc ttcgcgttcc tggaaaggat cttgtgacat ctcggttcct ccgttgtcat
    49861 ctctgatgaa gattttcgtc actccaccag caataattta taaagcggtt gattctcttc
    49921 aaccaaatcg gcaagcgtgt agttatccag ttccataaga aaactttgca cggccttaga
    49981 aagcgcctgt ttcaaccgac aggcaggtgt aatgtggcaa aactcactgc tgcaattcac
    50041 cagcgataag ggctccagct cgcgcaccac atcaccaata cgtatcgcac tcgccgattt
    50101 acccaggcga atgccgccat tttttccacg aacagcagtc acgtagccgg cacgactaag
    50161 ttgattgatt attttgacca tatgattacg ggagacgccg tagacgtcag tcacttcaga
    50221 aatactggtc atccgccctt ctggcaatga cgccatgtag atcagcgcac gtaatccgta
    50281 atcagtgaaa ctcgttaact gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt
    50341 tatattgatg ataaaccagc cacaagctgt gtcgctaatt tatttcagtt cggggaagga
    50401 aaaaagcgag gatttaacac tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt
    50461 atcacactgt tagcccggca ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa
    50521 cgggtcgcgc agaatcatgg tttcagtacg atccggaccg gtagagatga tatcgatcgg
    50581 cacaccagtc agctcttcaa tacgcttgat atagttcagc gccgcctgcg gcaggccgct
    50641 acgatctttc acgccgaagg tggattcaga ccagcccggc atggtttcgt aaatcggctc
    50701 tacacctttc cagtcgtcag ctgccagcgg agtggtagtc acttcgcgac catccggcat
    50761 acggtaagcc acgcagagtt taacctcttt caggccatcc agaacgtcca gtttagtcag
    50821 gcagaagcca gacagggagt tcagctgtac cgcacgacga acggcaacgg tgtccagcca
    50881 gccggtacga cgacgacgcc ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa
    50941 ctcgccagtt tcatcaaaca gttcggtcgg gaacggacct gcacctacac gagtggagta
    51001 agctttgagg atacccagaa cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc
    51061 cacgccacca gcagtggtgt tggaagaagt tacgtacgga taagtaccgt ggtcgatatc
    51121 cagcagcgta ccctgcgcac cttcaaacat gacgaaatcg ccacgctgac gcgcctggtc
    51181 gagcaggtca gaaacgtcaa ccaccataga agtcaggatg tcggcaacag ccatcgtatc
    51241 atccagaact ttctggtaat caaccgcttc agctttgtag tagttaacca actggaagtt
    51301 gtgatattcc atcacttctt tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc
    51361 aacacgcaga ccgcgacgtg ctactttatc ttcataagca ggcccgatac cacgaccggt
    51421 ggtgccgatc gctttcgcgc cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata
    51481 atcaaggatc agcggacatg cttcagacag cagcagacgc tcacgaacgg ggatgccacg
    51541 gtcttccagt tctttcatct ctttcatcag cgcggccgga gacagcacaa caccgttacc
    51601 gatgatgctg gttacattct cgcggagaat acctgatgga ataagatgga gaacggtttt
    51661 ttcaccgttg attacgagag tatggcctgc gttgtgaccg ccctggtagc gtacaacata
    51721 tttagcccgt tcagtcagaa gatcgacgat cttaccttta ccttcgtcac cccattgggt
    51781 gcccagtacg acgacgttgt tacccatttt tcaaaatcac cgtttgctta aaaatggatt
    51841 ctaccatcgc tttttcagag ttacagcact ttttgcactc aaaaatgacc tcgatcagtc
    51901 tgttttttgt tcagccaatc gttttcctca acatgtagta gaccacaacg cccgcaacca
    51961 caagtccacc gccaaaacga cgtaaaatat tatcgggcaa attggtcatc gcagagatca
    52021 tcttcttcca tgccttcggg taaagcatcg gccctaaacc ttccagtacc aaaaccaggg
    52081 caagcgccag ccagattgtc gaattcattc attatcctta tagaaaaaga aaaccaccga
    52141 catccctgag gatgcggtgg ctttattgac ctgtaccgca gtcgttatat taacgcgttg
    52201 cggaagtcgg cgtcttcata tagcggaaga aatcgctatc cgggctcatg accatcacgt
    52261 cctgattgcc agagaagctg ttctcataag cacgcaggct acggatgaat gcgtagaagt
    52321 ccggatcttt actgaatgca tcagcaaaca gtttggctgc ttcggcatcg ccttcaccac
    52381 gcatgatacg gccctgacgc tcagcttctg ccagcgttct ggtcacttca tagtcggcag
    52441 tcgcgcgcag tttttccgct tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt
    52501 cacgctcggc gcgcatacgg ttgtagatcg cttcagacac ttcggtcggc aggttgatct
    52561 gcttgatacg cacatcgaca acttcaatac ccagcgccgc catactgttc gggttgatga
    52621 ccggaacttt gcccttcgtc tcagccgtta cgcgctctgc cgcttcggca atggcgttat
    52681 ctgccgccgg ggtagtaact tcatcttctg tacccgcaga accggagttc agcgcgtcac
    52741 gtacttcgag ggtcagacga ccacgggaat cagtgacgat atctttcacg tccaggcgac
    52801 caatttcaga acgcagacgg tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa
    52861 tgtcgccacc acccgttgcc aggtagtaac ggctgaaatc gctgatgcgc catttgatgt
    52921 aagagtcgac gatcaggtct ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca
    52981 tggtctgaat acgtgcgtcg agcattttca ccgtttcaat gaacggtatc ttgaaatgca
    53041 gacccggctc ataaaccaga ggtttgttgt catcgtcacg cagtacctta ccaaaacgca
    53101 gcgtaatacc gcgctcacct tctttgacga caaagacaga catgtaaagc actaccagca
    53161 cgatgatgat aatcgcgata actgacttac gcatcgttat tccccctgac gctggtagtc
    53221 gttacgctgc gcgttggcgc ggcgttggtc cataatatcg ccctgactgg cggacgacgt
    53281 gttgcttgct ccactggttg tggaggaaga ggctggcggc agacgcagca gattgctggc
    53341 accgttatcg ctcttcgccg caggcgcatt gccgcctttc agcatctggt ctaacggcag
    53401 aaccatcagg ttgccacctt tatcgttaac cagcactttg cgggtgttac ccaacacttt
    53461 ttccatcgtc tcgatataca gacgctcgcg agtaatttcc ggcgcggctt tatattccgg
    53521 cagaagttta gcaaagcgcg ccacttcacc ctgagcttcc aggatggtct gggccttgta
    53581 cgcacgcgcc tcttcgagga tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt
    53641 ggtatacgct tctgcttcac gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc
    53701 gtcaaacgcc gcttttactt cttccggcgg acgagctgcc tggaagttga cgtccagcag
    53761 cgtgataccc atgtcatacg ggcggatagt ctcttccagt tcgcgctgag tatcgctacg
    53821 aatcacggta cgaccttccg tcagaatgcg gtccatggtg tatttaccga taactccacg
    53881 cagggcgctg tcggtagcct gacgcaggct gtcatccggg ctggtcacgc tatacagata
    53941 tttttccgga ttggtgacgc ggtactgcac gttcatctca acgcgcacta cgttctcgtc
    54001 cgacgtcagc atcacaccag aagcggccag ttcacgcacg gcttccacgt tcaccggttt
    54061 gacttcgtcg ataaacgtcg gtttccagtt cagacccggc tcaaccagat ggctgaattt
    54121 accaaagcgt gttaccacgc cgcgttcggc ttctttaatg gtatagaaac cactggccgc
    54181 ccagataatg acaatcgctg ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg
    54241 cgggccttgc gatgaactgc caccgccaga tccggtgcct ttaccgccgc ccagaccacc
    54301 gagctttttg ctcagtttgc ggaagatatc atctaaatca ggtggccctt gatcgcgacc
    54361 gcctttgttt ccatttccct cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg
    54421 gtcttgtccg ttattaccgg gctgattcca cgccatgttt gtgctccata tttgttatgc
    54481 ggtgatccct gttgtctttc gacgccagag gatatgactc cacgcttcag acgctacgcc
    54541 gttagatcag gtaatcgatc aacgccggtt cttgtttaca gaggcgacgc cagtcaacga
    54601 tcggcatacg aacttgcaga cttacgctgc cgtcctcctc catccactct ttttctattg
    54661 cctgaagctg ataaaaacga cttctcagac gcccttcctg cggtggtaga cgcaatgtat
    54721 gctgcgccac ctcgccggaa agccgctccg tcaaagcctg aaaaagctgt ggtatccccg
    54781 ctccggtctg tgcggaaagc cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac
    54841 gcggttcgaa atcttccagc atatcgatct tgttcatcac cagcagggtt gggatctcgt
    54901 gagcgtcgat ctcttcaaga accgtattca ccgcttcgat gttttcttgt acacgcacat
    54961 ccgccgcatc aatgacgtgc agcagtaatg tggcttgccg cgtctcttgt aacgtggctt
    55021 taaatgctgc caccagatcg tgcggcaggt ggcgaataaa ccctacggta tctgcaagta
    55081 cggtttcacc gacatctgca acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca
    55141 actggtctgc cgcgtagacc cgcgcttcgg tgatgcgatt gaaaagggtg gatttaccgg
    55201 cgttggtata tcccaccagc gaaacagtag gaacgtcggc tttgatacgc gattgccgcc
    55261 cctgctcacg ctgcttttca actctttcca ggcgcgactg tatctgcacg atgcgattac
    55321 gcaacaaacg acggtcggtt tcgagctggg tttcacccgg accacgcaaa ccaatcccgc
    55381 ctttctgtct ttcaagatgg gtccagccac gcaccaggcg tgtagccaga tggcgcagct
    55441 gcgccagctc aacctgcaac ttaccctcat gggtacgcgc acgttgggcg aaaatatcta
    55501 aaataaggcc ggtgcggtcg ataacacgac actcgcacaa acgctccagg ttacgctctt
    55561 gcgccgggct cagggcatgg tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt
    55621 ccgcaatttc aactgcttta ccttcaccta caaaatactt tgggtgcggc gctttacggc
    55681 taccggtaat cacctgcaat gcttcgacac cggcggaaga gaccagagat tcaaactcct
    55741 ggaggtcttc cagatctttg tcttgcgtaa aatagatgtg taccagtacc gcctgctcac
    55801 cagcatcata acggtcaaac aagcgtataa ccctctaaat agatcagcgg ggaacgcagg
    55861 atcgctggct ccccgtgtaa aaaaaacagc ccgaaacctt attcggtttc ttcgctgtcc
    55921 tgttgcgcgg aagtattctg cgcgctgcta ccatgatggt agttactgct ggtaccgcca
    55981 ccggcgttgt tactgtgatg agaaaccggg cgagacggga caacagtaga aatcgcgtgc
    56041 ttgtaaacca tctggctgac cgtgtttttc aacaggatca cgaactgatc aaaagactcg
    56101 atttgccctt gcagcttaat accattcacc aaataaatag aaactggaac acgttcccga
    56161 cgcagtgcgt tcaggaacgg atcttgtaaa gattgcccct tagccattct ctcttttcct
    56221 tatatgctta tttgtacttt gaacctttcg attctgaaaa aattgcgcac gatacgtctc
    56281 aattgtacac attcagcctg cgatagcacc aacaacctgt aatacttcgt cacgcgcctg
    56341 ttctggtttt tcactatcaa gccagtgaac cccttcccaa ccacgcagcc aggttatctg
    56401 ccgcttcgcc aactgtctcg tggcgcaaac acctcgataa accatttcat cgtatgagat
    56461 ttcgccttca aggtaagacc acatctggcg ataacccacg caacgaatgg aaggcaagtc
    56521 cgtatgcaaa tctcctcggg caaaaagcgc ccggacttct gcttcaaaac ctgaagccaa
    56581 catctgatga aaacgctgct caatgcgttg atggagcagt tcacggctcg ccggggcgat
    56641 ggcgaactga tgcacctgat acggtagagc gtctcctgac gtttgcgtca gttccgttaa
    56701 agttttaccc gaaatgaaaa aaacttccag tgcccgggaa agcctttgtg gatcatttgg
    56761 atgaatcctt gctgccgcaa ccggatcgat ctcctgaagt tgacgatgca atgactccca
    56821 accttgctct gccgcctgtt gctcaattct ggcccgtact tccgggtctg ccgacggtag
    56881 cggcgacaac ccttccagca atgccttgaa atacaacatc gtaccgccca ctaacagtgg
    56941 gatccgcccc gccgcggtga tataggccat ttccgccagc gcatcgcggc gaaaatcagc
    57001 agccgagtaa gcctgcgacg gatcgcgaat atccagcaat cggtgcggcg cggcgagtaa
    57061 ctcttcagcg ttcggcttcg ccgtcccgat atccatccct ttgtaaataa gggcagaatc
    57121 aacgcttatc aactctactg gtaaaatttt acgcagttca atggctaacg ccgttttacc
    57181 ggaggccgtc ggccccatca aaaaaatcgc cttaggcagg cccgccttac tgatatcact
    57241 catctttcag ggcttttatc gccggatgta aatcaacaga ttgtaacaga ccacccggcg
    57301 gcgttttcac aagttgcgga cataaccgtt ccacgtccgc cagcagggtt atggcctgtg
    57361 ccattgacca ctgcgcatgt tcgctcatca gatttcgtgc aatccactgc gcaatattgc
    57421 cagattcgaa tacggactgc ttcgccaggt agcctatcag ttcaggaatc aagatttgta
    57481 aattttgttg gcgtaagggt aaaggcacgg ccctgatggt cacatgctgt gcatctgact
    57541 ggaaatcaat acccaattcc gccagggcag actgcgcttt ttctaatgcc gatttttctt
    57601 cgccagaaac ttttagccgc aacggaatca gcaacggctg ggcgcaaacg ggcgcttcac
    57661 ccggcgtcaa ttgtgcctga cacagccaac gttctgccac tggcaaggct aaaagtgaaa
    57721 tgttgccgtc gcgctccagc aacgcacagt cggaatggac gatagtcagt acccgaccaa
    57781 aactctgact gttcgccgca agtgcaggtt cctgcggttc cggcgctttt ggtttttgca
    57841 tcggcgcggg cgtttgcaaa agctggcgat acacttcacc ttgctgtttc tggtagcctg
    57901 gctgcgcatt cggccagggg gcagccggac gactgcctga ggcgggcgca ggagtgtagc
    57961 gcggagctac cggctcacga gctgccggtt ctgcaaagtg attgcgcccc gccgccacgc
    58021 ggttttccgg aatggaacgc ggtgcaggtt ggggttcatc gtccagcggt agcggcgttt
    58081 ccagttgctg ttgtagcacg ctcagcacgc cctgatagat aaaatcatgc accagacgcg
    58141 actgatggaa acgcacttcg tgtttggcgg ggtgcacgtt gacgtccacc tgatgcggat
    58201 cgatctccag atacagcaca aatgccggtt gctgatcggc ccccagtttg tcttcgcagg
    58261 cctggcggat cgcgtgattg atcaggcgat cgcgcatcat gcgaccgttc acgtagcaat
    58321 actgaatttc tgccagtgcg ggcgtggtgt gatttggatc ggccacccag ccgcgtagcg
    58381 tgagatcgcc gtgttgccat tcaatcgcca gcgcttgttc aagaaaagcg gtgccgcaaa
    58441 tcgcgcctaa gcgccgttct ttttgcccgc cttccggcac tgcgcggtac tgacgcacaa
    58501 ttttaccgtt atgcgacagg ttgatcgtga cgtcgaaacg cgccagcgca atgcggcgga
    58561 tgatctcatc aatgtggtta aattcggttt tctcggtgcg caggaatttg cgccgcgccg
    58621 gggtgttgta gaacagatcc agcacctcca gcgtcgtccc cacaggatgc gccgccggtt
    58681 ttaccgtcac gttcatatcg cgcccttcgg cataggcctg ccaggcttcc tgctgttctg
    58741 cggtgcgtga agtgagcgtc aggcgggaaa ccgaactgat actcgccagc gcctcaccgc
    58801 gaaagcccag gctgataatg gcttcgagat cgtccagaga ggcgatttta ctggtggcat
    58861 gacgcgccag cgccagcgcc agctcgtctt ttttgatacc gcagccgtta tcacgaatgc
    58921 ggataagttt cgccccaccg cgttcgatat caatatcgat acgtgtcgca cctgcatcga
    58981 ggctgttttc caccagttct ttgactaccg acgcaggtcg ctcgaccacc tcacctgcgg
    59041 caatctggtt cgccagttgt ggcggtaaga cctgaattgg catcaatcgt ccttagtttg
    59101 gcagcgtgcg atctggcgtc gtcaccgtac tagccgtttg tgccgttgcc ccctgcggcg
    59161 cagattgcat cggatgcgca aggaaataat tgcgcaggcc tttgtaaatg gcttctgcca
    59221 gctgttgttg gtaatcgtcg ctcgccagca aacgttcttc gctgttgttg ctgataaaac
    59281 cggtttcgac cagtactgat gggatatccg gcgaacgcag aacgccaagg ctggcatgct
    59341 ctggccgacg tttatgtatt tcgccaatgc gttgcaactg actgatcata ctggtcgcta
    59401 catcataccc tacccgctgg gaatgaccga actgtaaatc cagcaccgcc tggcttaaat
    59461 aggggtcaga ctgactgttc gccagcacat cacccgcccc gcccagcagc tccgactgtt
    59521 tctcgtgctg ctccagccag ctggccattt cgctgttggc gcgacggtta gagagcaccc
    59581 atacggaagc gccagtcgca ctgcggttcg gtgcggcatc agcgtgaatc gacacgagga
    59641 aattggcgtt ttgcttacgt gccacatcgc tgcgccccat caccgagata aagtaatccc
    59701 cgtcacgggt taaaacgcct ttaaacatcg gatcgtcatt gagcaaagta cgcaatttac
    59761 gcgcgatggc gatggtgaca tttttctccc gcgtaccacc ggggccgata gcgccagggt
    59821 cctgaccgcc gtgtccggca tcaatagcga tgataatttt atcgccagtg ttagccgtcg
    59881 cgcgcgctgc cggacgcgtt accgtattac tgctgataac acccgtagtg cggttacttt
    59941 ccgttttaaa cggattgcgc gccggttcgc tgacgcgcgg tgcgacaacc gcaggcgttt
    60001 caacgcgttt cgcaaccacg ggcggtggag gaggcggtgg cggcacatcg gcgttaatcg
    60061 taaagacgac agtgtaattg ctgccattct gccgcttcac cgcttcggtt ttaccgtttt
    60121 cggtaagatc gaccaccagc cgtagcgttt gtgcatcttt aggcgttcca gagcgaatcg
    60181 ccttcaccag attattgccg ctgaacaaca acggcagtcc ctgaatcacg cccgtttgtt
    60241 tgatatcgag cgccacggtg cgtttgcttt gatggctaaa cgcataatca ggatcgccaa
    60301 taaaactcaa cgttatccgc gcctgttgat taccgttaga aacctgaata tcagagagcg
    60361 tcgcggcacc caccggcgtg cacagcagca gcgtcgctac caaccaattt ctgatgcgat
    60421 acatcatccc gccacctttc aaaggttaac cggctaaacg cgccagcaac aattcacccg
    60481 cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt
    60541 cgacatccgg gtcaggaaga acacctgtac cttgttgtgg ccactccacc aggcagatgg
    60601 catcgttggc aaaataatcg cgaatcccca taaactccag ctcctcggga tcggcaaggc
    60661 ggtacaaatc aaagtgataa accattaaat tgtcgagcgt atagggttcg accagcgtat
    60721 aagtggggct tttgacatta ccctgatgac ccagagcttg taaaaagccc cggctaaagg
    60781 tggttttacc tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt
    60841 tcgctacccg ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat
    60901 tcatcatggt ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga
    60961 tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgcagcaccg
    61021 tgcgcgacac agcccgcaca ggcggcatca tacggcgaca gtttttgccc aagcaatgcg
    61081 ccaataatac cagagagcac atcgcccatg ccgccgctcg ccatgcctgc atttccggca
    61141 tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc
    61201 gctacgccgc cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt
    61261 tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga
    61321 ttgtgacgct tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc
    61381 ggtttgcgaa aattctcaac tttttgcagt gcttttttcc cccactcttg ctggcccagg
    61441 ccgggaccaa tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatcgtc
    61501 agttcatgca ccatcaattc cggtcgtgca gtcagcagcg gcgcaatgtt ttcactgcgg
    61561 gtcagtactc ggactaaacc agcaccagca cgcagcgccg cttcccccgt catacgaata
    61621 gcccccgccg tgccgtgatc gccaccgata attaccagcc gcccgtgatc gcctttatgc
    61681 gaagtcgggc gacgcggttt tagccagtga gaaagttgtt ctgctgaaaa ccgctgaatt
    61741 ttcgtctcct gacctgccag ccaactatcc agccccagtg agtcaaaatg cagttgtccg
    61801 gtaacatccc gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg
    61861 tgatcggcgt tgatcaccgc gcctggcgta gcgccagttt cagccagcag gccggaaggg
    61921 atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg
    61981 gattcgcggg gcgcttgccg caaaccggta ccgagcagcg catcaacaat cagatctacc
    62041 gattcgggcc agacaatatt cgaagcatgg atctcgccac ccgcgtttaa ccatgcttcg
    62101 cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct cctgggccaa caacgtgacc
    62161 tcaatgccga ccgctttggc cagtcgcgcg accacgtagc catcgccgcc gttattacca
    62221 tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg ccgaacgaca cacctggaat
    62281 gcggcctcgc cagcgcgaag catcagctca tagagtgtga gccccagcac atctgccgcc
    62341 tcgcgttctc cgcggcggat atcgtcggcg taccagacgg tgtgtggtat acttacgggg
    62401 tttttcttca ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa
    62461 aaaattaaac agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat
    62521 ctcagcgagt ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg
    62581 gactggatgg cacgtcacgg tatgctgcgc gctcgccctc atgagttatt gcccggtacg
    62641 ctgcgcgtga tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg
    62701 ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa
    62761 cttctgcgca accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg
    62821 aattttagac cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgaaaaagct
    62881 gggctcggct ggacaggtaa gcactcactt atcctcaatc gcgaggccgg ttcgttcttc
    62941 tttttaggcg aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga
    63001 tgcggcaaat gcgtggcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat
    63061 accgtcgatg ctcgccgctg tatctcttat ctcaccatcg aacttgaagg ggcgatcccg
    63121 gaagagttgc gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcttatc
    63181 tgcccgtgga atcgctattc acaactcacc acagaagagg atttcagccc gcgtaagccg
    63241 ctacacgcac cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc
    63301 acggaaggat cggcgattcg tcgtattggt cacctgcgtt ggctgcgtaa tatcgccgta
    63361 gcattaggca atgccccttg ggatgaaacg attttgacag cgctggaaag tcgtaaaggt
    63421 gagcacccac ttctcgatga gcacatagcg tgggcgattg cgcagcaaat agagagacga
    63481 aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa
    63541 aaagggttac cgcgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat
    63601 tgcaattcaa gacgaataaa atcgtcaagt gatcgactcg acaaaacgga atcttaattt
    63661 ttaacttatc tatcaatagg ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat
    63721 taagattaca gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacga
    63781 aaaccagcaa taacgcatcg catcgctgtg gatattttat tgagagaaga atttggagcg
    63841 gg
//
LOCUS       DACAPB010000005        52077 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.005, whole genome shotgun sequence.
ACCESSION   DACAPB010000005 DACAPB010000000
VERSION     DACAPB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.005"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            66..335
                     /locus_tag="IT904_000271"
     CDS             66..335
                     /locus_tag="IT904_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP3190110.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            304..1422
                     /locus_tag="IT904_000272"
     CDS             304..1422
                     /locus_tag="IT904_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3190111.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1589..2383
                     /gene="potC"
                     /locus_tag="IT904_000273"
     CDS             1589..2383
                     /gene="potC"
                     /locus_tag="IT904_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAP3190112.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2380..3426
                     /gene="potD"
                     /locus_tag="IT904_000274"
     CDS             2380..3426
                     /gene="potD"
                     /locus_tag="IT904_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAP3190113.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3563..4402)
                     /gene="cobB"
                     /locus_tag="IT904_000275"
     CDS             complement(3563..4402)
                     /gene="cobB"
                     /locus_tag="IT904_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415638.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP3190114.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAG
                     SIA"
     gene            complement(4418..5329)
                     /gene="nagK"
                     /locus_tag="IT904_000276"
     CDS             complement(4418..5329)
                     /gene="nagK"
                     /locus_tag="IT904_000276"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAP3190115.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITAQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5358..6602)
                     /gene="lolE"
                     /locus_tag="IT904_000277"
     CDS             complement(5358..6602)
                     /gene="lolE"
                     /locus_tag="IT904_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAP3190116.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6602..7303)
                     /gene="lolD"
                     /locus_tag="IT904_000278"
     CDS             complement(6602..7303)
                     /gene="lolD"
                     /locus_tag="IT904_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAP3190117.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7296..8495)
                     /gene="lolC"
                     /locus_tag="IT904_000279"
     CDS             complement(7296..8495)
                     /gene="lolC"
                     /locus_tag="IT904_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAP3190118.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8757..9830
                     /locus_tag="IT904_000280"
     CDS             8757..9830
                     /locus_tag="IT904_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP3190119.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQYPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALLLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9958..13404
                     /gene="mfd"
                     /locus_tag="IT904_000281"
     CDS             9958..13404
                     /gene="mfd"
                     /locus_tag="IT904_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP3190120.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13548..14510
                     /gene="ldtC"
                     /locus_tag="IT904_000282"
     CDS             13548..14510
                     /gene="ldtC"
                     /locus_tag="IT904_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAP3190121.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14593..14850)
                     /gene="bhsA"
                     /locus_tag="IT904_000283"
     CDS             complement(14593..14850)
                     /gene="bhsA"
                     /locus_tag="IT904_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAP3190122.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            15091..15723
                     /gene="comR"
                     /locus_tag="IT904_000284"
     CDS             15091..15723
                     /gene="comR"
                     /locus_tag="IT904_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAP3190123.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15785..16324)
                     /locus_tag="IT904_000285"
     CDS             complement(15785..16324)
                     /locus_tag="IT904_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP3190124.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16551..17855)
                     /locus_tag="IT904_000286"
     CDS             complement(16551..17855)
                     /locus_tag="IT904_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP3190125.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18263..18805)
                     /locus_tag="IT904_000287"
     CDS             complement(18263..18805)
                     /locus_tag="IT904_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP3190126.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQS
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18828..19853)
                     /gene="nagZ"
                     /locus_tag="IT904_000288"
     CDS             complement(18828..19853)
                     /gene="nagZ"
                     /locus_tag="IT904_000288"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP3190127.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKAMAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAVIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(19864..20688)
                     /gene="thiK"
                     /locus_tag="IT904_000289"
     CDS             complement(19864..20688)
                     /gene="thiK"
                     /locus_tag="IT904_000289"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAP3190128.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHLQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20669..21310)
                     /gene="lpoB"
                     /locus_tag="IT904_000290"
     CDS             complement(20669..21310)
                     /gene="lpoB"
                     /locus_tag="IT904_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAP3190129.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(21324..21701)
                     /locus_tag="IT904_000291"
     CDS             complement(21324..21701)
                     /locus_tag="IT904_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAP3190130.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPITVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21704..22063)
                     /gene="hinT"
                     /locus_tag="IT904_000292"
     CDS             complement(21704..22063)
                     /gene="hinT"
                     /locus_tag="IT904_000292"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAP3190131.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            22397..24586
                     /gene="fhuE"
                     /locus_tag="IT904_000293"
     CDS             22397..24586
                     /gene="fhuE"
                     /locus_tag="IT904_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAP3190132.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFASYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(24646..26079)
                     /gene="ptsG"
                     /locus_tag="IT904_000294"
     CDS             complement(24646..26079)
                     /gene="ptsG"
                     /locus_tag="IT904_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAP3190133.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(26374..27171)
                     /locus_tag="IT904_000295"
     CDS             complement(26374..27171)
                     /locus_tag="IT904_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP3190134.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRELVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(27182..28186)
                     /gene="holB"
                     /locus_tag="IT904_000296"
     CDS             complement(27182..28186)
                     /gene="holB"
                     /locus_tag="IT904_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP3190135.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(28183..28824)
                     /locus_tag="IT904_000297"
     CDS             complement(28183..28824)
                     /locus_tag="IT904_000297"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP3190136.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(28814..29836)
                     /gene="yceG"
                     /locus_tag="IT904_000298"
     CDS             complement(28814..29836)
                     /gene="yceG"
                     /locus_tag="IT904_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAP3190137.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(29839..30648)
                     /gene="pabC"
                     /locus_tag="IT904_000299"
     CDS             complement(29839..30648)
                     /gene="pabC"
                     /locus_tag="IT904_000299"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAP3190138.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLVLSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(30768..32009)
                     /gene="fabF"
                     /locus_tag="IT904_000300"
     CDS             complement(30768..32009)
                     /gene="fabF"
                     /locus_tag="IT904_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP3190139.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(32097..32333)
                     /gene="acpP"
                     /locus_tag="IT904_000301"
     CDS             complement(32097..32333)
                     /gene="acpP"
                     /locus_tag="IT904_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP3190140.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(32544..33278)
                     /gene="fabG"
                     /locus_tag="IT904_000302"
     CDS             complement(32544..33278)
                     /gene="fabG"
                     /locus_tag="IT904_000302"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAP3190141.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(33291..34220)
                     /gene="fabD"
                     /locus_tag="IT904_000303"
     CDS             complement(33291..34220)
                     /gene="fabD"
                     /locus_tag="IT904_000303"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP3190142.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(34236..35189)
                     /locus_tag="IT904_000304"
     CDS             complement(34236..35189)
                     /locus_tag="IT904_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP3190143.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAQNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(35257..36327)
                     /gene="plsX"
                     /locus_tag="IT904_000305"
     CDS             complement(35257..36327)
                     /gene="plsX"
                     /locus_tag="IT904_000305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP3190144.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(36408..36581)
                     /gene="rpmF"
                     /locus_tag="IT904_000306"
     CDS             complement(36408..36581)
                     /gene="rpmF"
                     /locus_tag="IT904_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP3190145.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(36633..37154)
                     /gene="yceD"
                     /locus_tag="IT904_000307"
     CDS             complement(36633..37154)
                     /gene="yceD"
                     /locus_tag="IT904_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAP3190146.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            37353..37937
                     /gene="yceF"
                     /locus_tag="IT904_000308"
     CDS             37353..37937
                     /gene="yceF"
                     /locus_tag="IT904_000308"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAP3190147.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(38049..39008)
                     /gene="rluC"
                     /locus_tag="IT904_000309"
     CDS             complement(38049..39008)
                     /gene="rluC"
                     /locus_tag="IT904_000309"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAP3190148.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            39581..42766
                     /gene="rne"
                     /locus_tag="IT904_000310"
     CDS             39581..42766
                     /gene="rne"
                     /locus_tag="IT904_000310"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAP3190149.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQIETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGFVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(42962..43915)
                     /gene="flgL"
                     /locus_tag="IT904_000311"
     CDS             complement(42962..43915)
                     /gene="flgL"
                     /locus_tag="IT904_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAP3190150.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTDTAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(43927..45570)
                     /gene="flgK"
                     /locus_tag="IT904_000312"
     CDS             complement(43927..45570)
                     /gene="flgK"
                     /locus_tag="IT904_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAP3190151.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKLVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(45636..46577)
                     /gene="flgJ"
                     /locus_tag="IT904_000313"
     CDS             complement(45636..46577)
                     /gene="flgJ"
                     /locus_tag="IT904_000313"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAP3190152.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKVPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(46577..47674)
                     /locus_tag="IT904_000314"
     CDS             complement(46577..47674)
                     /locus_tag="IT904_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAP3190153.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMHVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(47686..48384)
                     /gene="flgH"
                     /locus_tag="IT904_000315"
     CDS             complement(47686..48384)
                     /gene="flgH"
                     /locus_tag="IT904_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAP3190154.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(48437..49219)
                     /gene="flgG"
                     /locus_tag="IT904_000316"
     CDS             complement(48437..49219)
                     /gene="flgG"
                     /locus_tag="IT904_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAP3190155.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGYKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(49391..50146)
                     /locus_tag="IT904_000317"
     CDS             complement(49391..50146)
                     /locus_tag="IT904_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAP3190156.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(50166..52077)
                     /gene="flgD"
                     /locus_tag="IT904_000318"
                     /pseudo
     CDS             complement(50166..52077)
                     /gene="flgD"
                     /locus_tag="IT904_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
BASE COUNT        12634 a        13977 c        12991 g        12475 t
ORIGIN      
        1 ttaatgttca accgtcgcca gttgtcgttg atattctgca actggcgcgt tccggagtga
       61 tagccatgag cgaacagtac ctgataacgc tcgacgagtg gaaaccaaaa cggttcagtc
      121 tcccaataac aaacactacc ctggtgaaat acggaaaact aggatacatc gttccaaggc
      181 cacaaaaaat tcgtgggcgt tggctgatag atcgccgagc agtatttgtt gggcctggtg
      241 aaacgggaat tgcgccggaa attcatactg gcgatgatga tgcactgaag gagattttaa
      301 ctcatgtcac cgaggccacg aaaaaacagc actgacgtag ccggtcttta cgaaaagttt
      361 gatcgcagaa ctggcagagt ttactaccag tataaaaatc ctgtgactgg aaaatttcac
      421 ggactcggaa cagacaaagg taaggcagaa aaaatcgctt ccacagccaa tcagcgaata
      481 gctgcagcag aagctgaata tttcatgcgc aaaattgatg aaagtccgtc agcaacaaaa
      541 cgtcggggta tcagattaaa ggcatgggtt gatcgatatc tgaaaataca ggacacgcga
      601 ctgaaaaatg gagatattgc agctacaact cacaaagaaa aaactcgaat ggctgcatac
      661 ctggtttccc gtctgggaaa ccacccattg aaagaactgg aagtaagaga ctttgcatta
      721 atactggatg agtggctgga taaagacatg gtcagcacag cgagagtaaa tcgtggatta
      781 tgggttgata tttataaaga agcacagcat gcaggggaag ttcctcctgg atggaatcct
      841 ccggaggcta cccgtaaacc gatccctaaa gtaaccagag ccaggctcac catggaagac
      901 tggcaaaaaa tttacaatgc aacgcctgaa aaacacttta tccgtaacgc aatgcttctt
      961 gcgattgtta ctggtcagcg ccgtgatgac atttgccaca tgcgtttttc agatgtgtgg
     1021 aacgaacact tgcatatcac ccagggaaaa accggaatgc gtctggcgtt accgcttaca
     1081 ctacgctgtg atgccattgg gataacgtta aaagaagtta ttgatgggtg ccgagacaga
     1141 atattaagtc catatctaat ccatagtcgg caccagaaac aaccgaagcc gatgagtaaa
     1201 gacaacctga gcgactactt tgccaaagca cgggatctgg ctgggataat tccaccagca
     1261 ggaaaaactc cgccaacatt tcatgaacaa cgctctctat cagaacggct gtaccgtgca
     1321 cagggtatcg atacaaaaac attactagga cataaagtcc aggcaaccac cgatcgctat
     1381 aacgatactc gaggtcagga atgggttaag ttggttattt gacaaacacc atatggaata
     1441 tccgtcacca tctcaggaaa aaagtgttgt aaaatgcgtg gcctagtttt ggggaaacgt
     1501 tttggagaga ttttggagaa aggaaaaaat tgaataaatt caaaatcctg aaagtgtccc
     1561 gccttatgaa taaaaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
     1621 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
     1681 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
     1741 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
     1801 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
     1861 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
     1921 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
     1981 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
     2041 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
     2101 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
     2161 aagctttacc ctgtcgatgg acgatgtggt agtttcttcg ttcgtcaccg gaccaagcta
     2221 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
     2281 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
     2341 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
     2401 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
     2461 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
     2521 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtatgcc
     2581 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
     2641 gataaaatgc gtaaagaagg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
     2701 agcaatctcg atccagacat gctcaataag ccgtttgacc cgaataacga ttactccatt
     2761 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
     2821 gtcaccagct gggccgatct gtggaagcct gagtacaaag gcagcctgct gttgaccgac
     2881 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
     2941 gatccgaaag agattgaagc tgcatataat gagctgaaaa aactgatgcc aaacgtcgca
     3001 gcgtttaact ccgataaccc agctaacccg tacatggaag gcgaagttaa cctcggcatg
     3061 atctggaacg gttctgcttt cgttgcacgg caggcgggta cgccaattga cgtggtgtgg
     3121 ccgaaagaag gcggcatttt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
     3181 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
     3241 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccggaa
     3301 gtggcgaacg ataaaacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
     3361 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
     3421 cgttaattca gcaagcgggc ggtggcatta ccgcccgttt tttatttagc aaggatgtgg
     3481 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccccttg
     3541 caggcctgat aagcgtagtg catcaggcaa tgcttcccgc ttttaatccc ttcagcaact
     3601 tttcaacaaa ctcaggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
     3661 taccaacctg actcggttca agattcagtt ccacggtgtg cgcgccatgc agtttcgctt
     3721 cgtgaacaaa cccagccgcc ggataaacat gcccggatgt gccaatggca atgaaaatat
     3781 cggccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaca
     3841 ctacatgcgg gcgcaagggt gccggaaact ggcagcaatg gcatttatct tctggggtaa
     3901 cgtctccggt ccagtcgaga acctgaccac tttgtgaaca acgaactttc agcagttccc
     3961 catgcatatg aatcacattg gtattacctg cgcgttcatg caggttgtcg atattctgcg
     4021 tcaccagcaa aaagcgatcg ccgagggcgt cttgcagttt agccagcgca agatgcgcgg
     4081 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
     4141 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
     4201 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
     4261 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
     4321 aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt tttacgaaaa cgacttaacc
     4381 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
     4441 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
     4501 ctacaggtaa gagatgacga ggcagcctgt ccgccagttg cgctgtgatt gccgggaaat
     4561 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
     4621 gacaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
     4681 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
     4741 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
     4801 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
     4861 ggccaaactc gccggtaatg tagcttttac cggtaatcgg tttgccgttg aaaatcagcc
     4921 cgccgccaac gccggtgccg agaatcaacc ccatcaccag tggatattga gtaaattcat
     4981 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
     5041 caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct ggcagcgggg acattggcgg
     5101 catacagcgt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
     5161 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
     5221 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
     5281 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
     5341 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
     5401 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
     5461 aagacgtcca gccagtgcaa ttccgatggc aggaaatcaa taaaatagat atcgctggag
     5521 aggaactgat gaccgatcaa cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
     5581 accactacgc cgataatcac accacacagg ctgccgaaca gccctgccag caatccatac
     5641 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
     5701 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
     5761 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatttggat atcgcgatac
     5821 atatagccgt aagtaccaat ccagctttta atataaacat agctgttggt cacttcaccc
     5881 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
     5941 acgctggaac ccatatcaag atattgttgg gcatctgcca gcgggatcat ggcaaaactg
     6001 tgatcgagtt gaccactcaa ctgcaaaata ccggcaacgt gcaaacgcac acgttttggc
     6061 tgcatcagtt tatgctcggg attcgagttg gggatcataa tcgacaccca atccccctgc
     6121 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
     6181 aaattgcgcc acgcatcgcc ctggacaaac gagggtaatg cgctcagacg ctgttcctgt
     6241 tgcgggttaa cgcccttcac ctggattgcg cgaagattcg cgccactttc caccagcccg
     6301 gtgaaattga tatacggcgc ggcagcggca atacccggca ctttttgcac gtgatccagt
     6361 gcttcctgcc agttagtcca cggctgatcc accgcctcga tttcaccatg cggcaccacc
     6421 gccagaatac ggttgttcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
     6481 aataccgcca cgccgagggc aatgccaatg gtagaaatga cggagatcag cgacaccatg
     6541 ccgccgcgcc gccgtccgcg actaaaacgc aggccaatta ataacgataa aggcatcgcc
     6601 attactccgc ccccatcagg ctcagttccg ccgtcagacg accatcacgc atttctaact
     6661 ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac caccaggaag gcggtgccct
     6721 gcaagcgatt caattcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
     6781 taccggtagg ttcatccgcc agtaccaggc gcgggttatt caccagcgca cgggcaatcg
     6841 ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgatcca
     6901 gccccaccgc ttttaacatc tcaagtgcac ggctgttgat ttcagcgggc tttttcttgc
     6961 caatcagcag cggcatagcc acgttttcca gggcggtaaa atccggcagc aggtggtgaa
     7021 actgataaat aaagcccagc ttctggttgc gcagttcagc tttcgctgcc gacgacagtt
     7081 tgctcattgg ctgaccatta aaaatcacat cgccggaggt tggcgtatcc agcccgccca
     7141 gcaggtgcag caaggtactt ttaccggaac cagagctacc gacgatcgcc atcatttcac
     7201 cttcgccgac gctgaaactg acattgtgca gcacatcggt ttgcacactg ccttcctgat
     7261 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
     7321 ggttgagtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
     7381 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
     7441 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagggcgcca
     7501 agcgccgctc cgaggatcgc accgataatc ccggcgctgg ccccttgcac cataaagacc
     7561 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgctacttc gccctgcttc
     7621 tccattacca tcagccctag tgaggtaata atgttaaacg ccgcaacggc gacaatcagg
     7681 ctcagcagta aacccatcat atttttttcc atgcgtacgg cctggaacaa ctcgccttta
     7741 cgatcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taatgagtcg
     7801 actttcagcg gctcatccag ccacaaacgc cagccggtaa tattgcctgc cggataacgc
     7861 atcagacgcg aggcatcctc aatattcacc agcatttcat tgccatcgac ttcactgttg
     7921 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
     7981 aactggctgg cagatggtac catcacgcgg atttgatcac cgcgattaac gcctagctgt
     8041 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
     8101 acattgacca gatacggtgt aagtggatct ttttgcgccg ggtcgatacc gagcatcacc
     8161 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
     8221 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
     8281 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
     8341 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
     8401 atggtagaaa gccaggagac gaaacgaccg aagcgatccg ctgcacgccc acgcatgtaa
     8461 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
     8521 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
     8581 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
     8641 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacata
     8701 gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gtcctgatga
     8761 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
     8821 atcactcggt cattaccttt tatccgcatc tgaccacttt tcagtatccg ttatcggaag
     8881 tcctgagcaa atgctggatc tatttcaatc tttaccttgc accctttcgt atgccggttt
     8941 ttttctttat ttccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
     9001 gtctcgataa acgcatctgg agcatctttt gggtgctggc gctttggggc gtggtgcagt
     9061 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
     9121 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
     9181 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
     9241 gtctggcgct gctactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
     9301 ccacgccgtg gtggggaatg aacagtgtga tccgcaattt gccttattac agccttggcg
     9361 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
     9421 taatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
     9481 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcaatac gagcaacgat
     9541 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
     9601 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
     9661 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
     9721 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
     9781 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctcccgctaa gccatcgccc
     9841 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
     9901 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
     9961 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    10021 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    10081 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    10141 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    10201 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    10261 cagcgtggcg tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    10321 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    10381 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    10441 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    10501 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    10561 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    10621 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    10681 gaacatattt accagcaagt gagtaaaggc acattacctg ccgggatcga gtactggcag
    10741 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    10801 ctggtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaggctga cacgctggcg
    10861 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    10921 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgggtgca gctaaaaact
    10981 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    11041 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    11101 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    11161 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    11221 gggcgttatc taatgattgg cgctgccgaa catggttttg tcgatacggt gcgtaatctg
    11281 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    11341 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    11401 ccggtggtcc atctggagca cggtgtcggg cgctatgccg gaatgaccac gctcgaagcg
    11461 ggcggcatta ctggcgagta tttgatgctc acctatgcca acgacgccaa actgtatgtt
    11521 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    11581 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    11641 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    11701 gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg acagcttccc gtttgaaacc
    11761 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    11821 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    11881 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    11941 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    12001 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    12061 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    12121 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    12181 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgc
    12241 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    12301 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    12361 atcctgcgtg aaattttgcg cggggggcag gtttattatc tctacaatga tgtggaaaac
    12421 atccagaaag ctgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    12481 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    12541 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    12601 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    12661 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    12721 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggtgca
    12781 ggctttgcgc tggcaacgca cgatctggag atccgcggcg cgggtgaact gcttggcgaa
    12841 gaacaaagtg gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    12901 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    12961 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcattcc tgacgtgaat
    13021 acgcgtttgt cgttctataa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    13081 atcaaagtcg agcttatcga tcgcttcggc ctgctgccgg atccggcgcg taccctgctg
    13141 gatgttgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    13201 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    13261 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    13321 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    13381 ctggaagaga acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc cccatctgca
    13441 acatttacaa attctttgca cttccctgca ctatccgaca ctgtcaccat ccataattca
    13501 ggcttatctg tttattacaa taaccttata tttattatgg atttttggtg atgatcaaaa
    13561 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    13621 cggcaaaagc caacacctgg ccgctgccgc cagcgggcag tcgtctggtt ggcgaaaaca
    13681 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    13741 tcggctttct cgctctgtta caggctaacc ctggcgttga tccttacgta ccgcgcgcgg
    13801 gtagcgtgtt aacgatcccg ttgcaaaccc tacttccaga tgcgccgcgc gaaggcattg
    13861 tgatcaatat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    13921 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    13981 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    14041 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    14101 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    14161 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    14221 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    14281 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    14341 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    14401 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    14461 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    14521 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    14581 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    14641 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    14701 cagcgatccc agatttgtcc ccgcgttagc actgattgta ccgacttttt gttggccttc
    14761 tggcgttgac tgaacttcga cagccgcaaa gctggcaaat gacatggagc ttaaaatcgc
    14821 cgcagcgatg agggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    14881 gcaagcaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    14941 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt taaatagaaa
    15001 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    15061 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    15121 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    15181 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcgaccgga
    15241 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    15301 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    15361 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    15421 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    15481 ggagatatcg ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    15541 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    15601 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    15661 ctggaaaaac taatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    15721 taagtcgcaa aaaaagcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    15781 cttgttatac tttgttattc aaaatcagtt gcccattact atctagcggg atctgcgtac
    15841 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    15901 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    15961 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    16021 cccccacaac agtggcgaca tctttaccgc gaccaccgcc aaactgatgc cctatcacgc
    16081 cgccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    16141 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    16201 gggttgcaga aacaacctga gcgtattgcg ggccacgttc aaacacgttc agactggcca
    16261 ctgccgctac gcccagcgca gctgcgacac caatcccgat acccgccaac attgatttat
    16321 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    16381 ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa tacttaatga ttcggaggag
    16441 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgaaccat
    16501 ttgtccaggg cggatgaggc gtttatgcca catccgccag tgtacgtcga ttaatgcaac
    16561 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    16621 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    16681 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    16741 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggt
    16801 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    16861 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    16921 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    16981 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    17041 cataccatca gatccgcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    17101 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    17161 gccgcagcag agatacgtgg cggtaacgca ggcaaaatac gttctcccgc ttctaccagc
    17221 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc
    17281 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    17341 ttcactttgc cattcgcgcc caggttggcg gagtatttca ggaagagatt cagcatctcc
    17401 tggtgaaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacaccc
    17461 ggcgtattga aatcgttaga ggtgctaccc agcgccatta ccagggtgtc ataggcgatt
    17521 ttacgttccg gaaccagcag ttcacctttc tcatcgcgca gttctgcaat agtgattgtt
    17581 ttcgcttcac gatcaatatc aatgacggaa cccagctgga actggaaacc atgattgcgc
    17641 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    17701 tgcagcagcg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    17761 ttcttgcgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    17821 ccgacaatca caattttttt caatggcgta gtcaacgtga cccccttaaa tttattaacc
    17881 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    17941 atgttcttcg ctattgagaa taacatgaat ggtgcattgg ttataccaaa attgatgtta
    18001 atcaagtttt gttgctgaaa agataggcga ctttaccggg tgagatcaaa aaacagagtt
    18061 tattgtttat tatttgagcg aatccgaaag ggcgatatag ggtgaagcgt atatcgaggg
    18121 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    18181 atgcggcgtg aacgccttat ccgacttaca gaattcggag tgcatcaggt accggcaagg
    18241 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgcaaat
    18301 gcggggagat attcttgaat ttgtgcgact gctcttcgtc ccagacaatc tcgtaataat
    18361 gatgcagctc ttcagatgtc cgctggctgt taagcgcttc atcattacgc gacaaaatca
    18421 ccaggcaacg atcgcgattc ttctcacgga agttggtcac acacttagtg gcaatatcgg
    18481 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    18541 tgaagatcac ctggcggatg tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    18601 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    18661 ctttgagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    18721 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    18781 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct
    18841 ttctcttcct gccagcgttc atgtaactga ttcagacggg cgctgatcgc tttccagcga
    18901 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    18961 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    19021 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    19081 ataatcacgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt
    19141 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    19201 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    19261 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    19321 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    19381 attccggctt catgcatacc atcaataaac cggctggcaa ttgccatggc tttttgtgga
    19441 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcacaggc
    19501 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcaacca acctgcctcc
    19561 tgcgccagtt tgccaccctc ttccattcct gacagcgcag cgaatgattg cgccgctggc
    19621 aagcgggtaa aaccttcacg aaaacgctgc acgcgtccac cttcctgatc aaccgccacc
    19681 accagatgat tgcgcgaagc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    19741 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    19801 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    19861 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    19921 atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct ttgagcatca gcagccaggg
    19981 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    20041 gtcattgacc aattgcaggt gctgttcagt attttccacc cacaccgccg ccagttccag
    20101 cgcgatatca ccatctcctg catactccca gtcgatgagt tttaaccctg acgcgctatg
    20161 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gccgtggttc
    20221 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc actgtccgcc gcgccggatc
    20281 gctttgctgc cagtacagtt ccagtaacgg caacagcgtt attcgccagc caaaacgtgg
    20341 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    20401 taccgcgccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    20461 tgcaatgcat gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    20521 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctggtgtt caatgagaaa
    20581 actcccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    20641 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    20701 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    20761 tgccagaggc gctggagtat agcacgtaat gagcgccgac attgcgggca atgcctatgg
    20821 ctttactacg ggtacctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    20881 acagctgctg ggcggaaacc agggtaaatt taccgttatt agccagcgca tttcgcagcg
    20941 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    21001 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    21061 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgcagtt tgatcttcgt
    21121 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    21181 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    21241 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    21301 tttttgtcat caagattcac cccttacaaa tatagataaa gtctgacttt gtgcgccccc
    21361 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
    21421 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
    21481 acggtaatgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg ttgaatatca
    21541 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
    21601 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
    21661 aacaacgcca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
    21721 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
    21781 gtccgccatg gcggttggtg ttcatgatca gacgatagcc atcttcggca ataccttctt
    21841 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
    21901 acacgtcgtt cacagtcggt atgaggatat tcggaatgat cagaatatgc gttggcgctt
    21961 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
    22021 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
    22081 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
    22141 gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
    22201 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
    22261 ttggtttaca cgacaaatat gaatgcgtat atttctcatt ttcatttaca aacaaaatta
    22321 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
    22381 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagccat
    22441 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
    22501 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
    22561 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
    22621 acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
    22681 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
    22741 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
    22801 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactctt
    22861 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
    22921 tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc
    22981 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
    23041 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
    23101 ggaccgctac aacagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
    23161 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gccctacctg
    23221 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gtgcacgcag
    23281 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
    23341 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
    23401 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
    23461 gccatacagt aattatggac ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg
    23521 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt atttatgaat tgtttggtcg
    23581 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt acttcagttc
    23641 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
    23701 acaaaccgac tggtcaccac agagcctggc acaggacgat accacacata tgaaatcgtt
    23761 atatgctgcc actcgagtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
    23821 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
    23881 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
    23941 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
    24001 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc
    24061 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
    24121 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga
    24181 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatattgc
    24241 agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt
    24301 cgccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca
    24361 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
    24421 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
    24481 gaacgtcaat aacctgttcg acaaaaccta cgataccaac gtggaaggtt ctatcgtcta
    24541 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
    24601 aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acagattagt ggttacggat
    24661 gtactcatcc atctcggttt tcaggttgtc ggatttagta ccgaaaatcg cctgaacacc
    24721 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccggcct gatccacttt
    24781 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
    24841 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgtcgcttt
    24901 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
    24961 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga acagccacag
    25021 tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc
    25081 gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat
    25141 gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg tgataccggt
    25201 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg
    25261 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa
    25321 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
    25381 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
    25441 gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
    25501 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
    25561 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
    25621 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
    25681 gttaaacatg tacgctgcga tcgcaccgga gataatccct ccgagtacgc cagtatccgc
    25741 caggtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
    25801 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
    25861 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
    25921 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
    25981 accgacgccc agcagaatac ctgcgatagg cagtacggat accggcagca tcagcgattt
    26041 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
    26101 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gatcctcgcc gtgtacaggg
    26161 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac
    26221 gactatcacg tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt
    26281 cacaatattt caaacgtggt tattcaccgc caaattttcg gcggtgaact ttactcgttt
    26341 tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
    26401 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
    26461 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
    26521 ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca gtaaccgatc
    26581 cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc
    26641 ggaaaaggag atgtaaaatc cgagatccag taatttgccc gccgtttctc tgtcctctgt
    26701 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
    26761 atcggcgcgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
    26821 gatatgatgg atgaaggact cttgctgacg aactttagtt tccggtgtgt aataataatc
    26881 cagcccggtt tctcccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
    26941 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
    27001 acgttcgcct accagctccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag
    27061 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
    27121 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
    27181 cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta
    27241 aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa
    27301 tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt
    27361 cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta
    27421 gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa
    27481 gggccgccag cagcgaatac cagtcgcccg atggtacgct atatgccaac gcctgacaca
    27541 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
    27601 cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa
    27661 gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga caacgactac
    27721 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
    27781 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggttagt aaggcagcat
    27841 cggttaccca gacaactttc gcaccaccta agcgtgcgtg ctcattcagc ttttcggtga
    27901 cctcacgtac cgcatcaatg cccaacgcat ttttcccttt ttcgggagcc agggtgtagt
    27961 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
    28021 gaccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc
    28081 ccatgcccgg taacgcctga atgagcagcg catggtgacc tcttccggcc tgatagctgg
    28141 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    28201 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    28261 atcaatggta tgaatgcttt tatcttgtgc tgccagttcc agatagcggg cgcgggtgcg
    28321 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcacg
    28381 ttttaagcca acttccgggg taacatcgag atagagcgtt aagtcggggc gaaaatcacc
    28441 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc cgccgccctg
    28501 atacgcctga gtggagagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc
    28561 aggtttgatg actgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    28621 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    28681 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    28741 ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    28801 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    28861 acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    28921 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    28981 tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    29041 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg
    29101 ggtcggtctg caagcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    29161 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    29221 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    29281 ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatcc
    29341 aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg
    29401 ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc tcacgcaatt
    29461 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    29521 taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    29581 tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga aaaacccgtg
    29641 ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt ccggtccctg
    29701 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    29761 ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac aagattatca
    29821 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    29881 cgttgctgac gaaaaggaga catcgccaca ggcacgtacg ggcatcactg gcattaacgc
    29941 attacaaata accatctcat ctgcctgcaa cgcctcttcc agagaagctt gcacttcgac
    30001 aagctgataa gaggattgtg ccagcaaacg gatacagaat tgtcgcataa tgccgttaac
    30061 acctgcctga tccagtcgcg gcgtataaac tacgttgccc ttccgccaga acaaattagc
    30121 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    30181 ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    30241 aagatgagga ttgcgcccca gccgcaccgg gcttagcacc aacgtcatcc cctcgttacg
    30301 caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg ctggtccgct
    30361 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagtacacc
    30421 attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca
    30481 ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg ataacaaact
    30541 gactttaccg tcgataactc tggcagtggt aaaacaacca tcaccaaact gcgttgcccg
    30601 atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta
    30661 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    30721 aaaaaaggcc cgcaagcgga ccttttatat gggtgggaaa tgacaactta gatcttttta
    30781 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
    30841 ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta
    30901 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagac
    30961 tctactgccc ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    31021 acacggcttg cagcttcacc gaagatggct ttcaccgcct gcgcttcagc tttatcgcca
    31081 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    31141 tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    31201 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    31261 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    31321 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    31381 gataatgcac gtgccgcgcc aaaaccacca acacccagcg gcgtactggc tttctctgcg
    31441 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    31501 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    31561 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    31621 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    31681 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
    31741 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    31801 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    31861 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    31921 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    31981 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa aaaatgattc
    32041 tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac
    32101 gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct
    32161 tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca
    32221 agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta
    32281 acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta
    32341 aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat
    32401 ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc
    32461 caataacgca aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca
    32521 tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt
    32581 tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc
    32641 tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg
    32701 ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata
    32761 ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc
    32821 gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata
    32881 atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca
    32941 gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg
    33001 ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa ttccgcgcga
    33061 attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg
    33121 ccgttagcac ctaaataatc actgatcgcc tgagcgccat tttcactggt cgcagtgcca
    33181 ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt
    33241 gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt ttaaagctcg
    33301 agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg
    33361 cgtttcgtca ggccagtaag cactttgccc gggccgactt catagagatg ttctacgcct
    33421 tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc
    33481 agtgcgtcac ggatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca
    33541 ggaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct
    33601 ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc gcgttttgcg
    33661 cccgcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc ggcaataacc
    33721 acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc agcttcttca
    33781 cacgctttcg caatagacgc atcatccaga ccgatgattg ccgccatagc gcccgtgcct
    33841 tccggtacgg cttcttgcat gaacttgccg cgcatctcaa ccagacgcac cgcatcagcg
    33901 aaatcaatca caccagcgca aaccagcgcg gagtattccc ccaggctgtg accggccatc
    33961 attgccggtg ctttaccgcc ctgctgctgc catacgcggt acagcgcaac agatgcagtc
    34021 aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgggtc
    34081 agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc ttcgacaatt
    34141 ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac
    34201 acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcggagccc
    34261 caggtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt aatgcgcccg
    34321 tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt accgtggcga
    34381 tccagcgtca ccacgacatt gtccatagac ataccgagtt ttttcgccgt tgcactgata
    34441 atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattt
    34501 gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg
    34561 ttgcccgcca tcgtcagatg aattgaattc tctggattca cacggtcagc attaggcagc
    34621 gtcagcaact caccatagct accgtcggca tgcagatggg tggagatgat tcccggctct
    34681 tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat agtcccacga
    34741 tcggttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc
    34801 gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcgcaggct
    34861 gctgcaacgt caaatgccgg gcaaccttta atgcccagca tgctttgaat ctgacaagct
    34921 gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc aatctggtct
    34981 ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg
    35041 gtttcgtttt gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg
    35101 tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag
    35161 ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg
    35221 tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc
    35281 accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg
    35341 aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg
    35401 attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt
    35461 atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt
    35521 taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag
    35581 gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa agccgtcaca
    35641 aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt
    35701 gatagaaggg attgttttaa gcaccgctga ggcatcccga atactgtcga gaccctttac
    35761 ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc
    35821 agccagaact gagcccataa tggcaaattg caccaacatt gtactgtcac aatcgacgtt
    35881 ggcccctaag tcaaggacca ccgttttgcc cttttgctga tgtggtaata ccgtcaccag
    35941 cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagtgc
    36001 cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc
    36061 cacgcgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg catcactggc
    36121 gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt cagctttagc
    36181 aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga
    36241 attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac
    36301 atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca
    36361 gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg
    36421 accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca
    36481 gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg
    36541 ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta
    36601 cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg
    36661 gcaaatgggt ttggcttttg cacttcttca ggcagttcac caaagaccat gtccgcttcg
    36721 gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca
    36781 tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac
    36841 gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg
    36901 tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc
    36961 gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc
    37021 acatcactgt ccacactgac tacggattcg gcgacacgct caacctgatc aggggtatag
    37081 ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat
    37141 tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag
    37201 gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt
    37261 cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta
    37321 tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct
    37381 cgccatggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcaccag
    37441 aggtcgacga aaccccacgc agcgacgaat caccgcgaca gttggtgctt cgactggcac
    37501 aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc
    37561 aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc
    37621 tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg gcgttgttta
    37681 attcggcgaa tgggcatctg caaacagaag tggagccttt tgacgtccat ttccgtcacc
    37741 tgagcgaggc ggagattgat aattacgtgc gtaaggagca tcccctgcac tgcgcgggta
    37801 gctttaagag tgaaggattt ggcattacgc tgtttgagcg tttagaggga cgtgacccta
    37861 acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa
    37921 acccgctgat gggatagttg tttgcgccgg atgcggttac gtcgcatccg gcaatcggtg
    37981 caggcggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat caggcttata
    38041 tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc ttcatccatc
    38101 ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa cgctgcggcg
    38161 tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
    38221 tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat actgtgtatg cacacggatc
    38281 tgatgagtac gtccggttac cgggctacaa cgcaccaggg tggcaaatgc atagcgttct
    38341 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tgacacgcac gatacgttcg
    38401 ccgctttgca gaatattttt caataacggc gcttgaacgc tcttcacatg cgactgccac
    38461 tgaccgcgca ccagcgccag gtaatctttt tgcatccctt tttcacgtaa ttgctcatgc
    38521 agagaacgca acgccgagcg ttttttcgct accagcaaaa cacctgaggt gtcccggtca
    38581 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcaata
    38641 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
    38701 aggatgtgat catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
    38761 ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtggaatacg cacctcatca
    38821 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcgccttta
    38881 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg caaaaagtta
    38941 tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga tggagtctct
    39001 gttttcatgg ttggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
    39061 ttatatttac ttttgcacct tattacttca ctgcgtgatc actttattga tggttattaa
    39121 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
    39181 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
    39241 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
    39301 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
    39361 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
    39421 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
    39481 ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
    39541 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
    39601 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct
    39661 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
    39721 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
    39781 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
    39841 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga
    39901 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
    39961 ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct cgccgtatcg aaggcgacga
    40021 ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca tggggcttat
    40081 cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcactg caatgggatt taagcttccg
    40141 tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggccc cgttcctgat
    40201 tcatcaggag agcaacgtaa tcgttcgcgc tttccgcgat tacttacgcc aggacatcgg
    40261 cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgtcagcata tcgctgcatt
    40321 aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag
    40381 ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc gtctgccatc
    40441 cggtggttcg attgttatcg acagtaccga agcgttaact gccatcgaca tcaactccgc
    40501 acgcgcgacc cgcggcggcg atatcgaaga aaccgcgttt aacaccaacc tcgaagctgc
    40561 cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt
    40621 catcgacatg acgcctgtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
    40681 gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat
    40741 gtcccgtcag cgcctgagcc catcactggg tgaatccagt catcacgttt gtccgcgttg
    40801 ttctggtact ggcaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtctgat
    40861 cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat
    40921 cgcttcttac ctgctgaatg aaaaacgttc tgcggtgaat gcgattgaaa cgcgtcagga
    40981 cggtgttcgc tgcgtgattg tgccaaacga tcagatagaa accccgcact accacgtgtt
    41041 gcgcgtgcgc aaaggggaag aaacgccgac cttaagctac atgctgccga aactgcatga
    41101 agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc ctgaacaacc
    41161 tgcactggca acctttgcca tgccggatgt gccgccagcg ccaaccccag ctgaacctgc
    41221 cgcgactgtc gtagccccag caccgaaagc tgcaacggca acaccagcag ctcctgcaca
    41281 acctgggcta ttaagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    41341 agccaaaccg accgagcaac caacaccgaa agcagaagcg aaaccggaac gtcaacagga
    41401 tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    41461 cgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca
    41521 gcagcagact gccgagacgc gtgagagccg tcagcaagct gaggtaacgg aaaaagcacg
    41581 taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    41641 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    41701 cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    41761 gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg tggttgaaga
    41821 aactgccgct gccgaaccaa ttgttcagga agctccagct ccacgcacag aactggtgaa
    41881 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    41941 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    42001 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccagt cgccaatgcc
    42061 gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc
    42121 aattgtacgt ccacaagatg tacaggttga agagcagcgc gaacaggaag aagtacatgt
    42181 gcagccgatg gtgactgagg tccctgtcgc cgccgctatc gaaccggttg ttagcgcgcc
    42241 agttgttgaa gaagtggccg gtttcgtaga agcccccgtt caggttgccg aaccgcaacc
    42301 ggaagtggtt gaaacgacgc atcctgaagt gattgctgcc gcggtaactg aacagccgca
    42361 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacaag cagaaccggt
    42421 ggttgaaccg caggaagaga cggcagatat tgaagaagtt gtcgaaactg ctgaggttgt
    42481 agttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtagtcgctg aagtcgcagc
    42541 agaagttgaa acggtagctg cggtcgaacc tgaggtcacc gttgagcata accacgctac
    42601 cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga
    42661 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    42721 acatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaaag
    42781 taatcaagcc ctggtaactg ccagggcttt tttatttcat ctttgaatca acatattaaa
    42841 attcatcgaa tggcatcctt gctaaccaac aatgcaaaat aggcaaaaaa atgcccgccc
    42901 cagcgatccc agagcaggca gacaaaacat acccagtttc atgatatgtt ttaaagcgaa
    42961 attatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    43021 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    43081 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaat gaatccagcg
    43141 actccagttc gttcagctgc gtgcctaatt ccgcgcgaac agtcagcaca ttgttcagcg
    43201 agtttttcag tccgcggttg gttttatcta acgccgcagc ggcggtttct ttatccgctt
    43261 cgctatccgc gaccggcgtt ttcagggctg cgatggcact atccagcatg gcaaaaagat
    43321 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt gctggtaata ctgtcgaaaa
    43381 ttttgtcacc cgtgtgccct atcaccatcg aacgcgaagc atcgacctgt tgtttaatac
    43441 tttctgctcc accgacgtat ttccctttct cttcgctaaa cggcgcagta tctgttttat
    43501 aaccggcaaa aatgtagcgc ccgttaccgt cagtggtgtt cgccagattc agcaactggt
    43561 cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc aaagtgccat
    43621 tgctggcata gacaattttt tcctgagcat tctggatagc agtggtgacc tggctaagta
    43681 cactctcttc cagtgacact ttttgagtgg cgaaagttcg cgccagcgtg tactggctgt
    43741 tttgcgcctg tgcctgggag agaactaccg cttgtgatgc tgcaatggga tcgtcagaag
    43801 ggttaacgac tcgcttaccc gtcgacatct gctcgccgta cttcatccat tctgcctgag
    43861 aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa cgcattttat
    43921 ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg
    43981 cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt
    44041 gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggt
    44101 gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc
    44161 gtcgttaaag gatttcgctc cgcccaccgt tttactgttg ctttgcagat ccagcagggc
    44221 ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg cgattttcgc
    44281 ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactagtt tcagcgtgaa
    44341 gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt
    44401 accgttggca tctggtgtca ccgtaaaagt ggtattgctg gcaaggcggg tgacctgcca
    44461 ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt accacggagg catcagttac
    44521 cgtggcaccg atcgcaacgt taccgttgtt tttagtgttt tgcagaaccg cgggcttacc
    44581 gatagcaaag aaatcttcac cctcatcgcc gttggcatca aatccggctt tgtgttggct
    44641 gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg tctggtccag
    44701 atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg
    44761 gatctcaata ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc
    44821 gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt
    44881 ggccatcgtg atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg
    44941 gtttaattcg ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg aggcccctgc
    45001 ccccacgcct gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt
    45061 gttgatctga tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag
    45121 atattgatcg gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg
    45181 gcgcgctgcc gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg
    45241 catctgtgtt gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg
    45301 ctcatagcgg gcagtcaggc cgctactttg cgtctgcgcc gcacgtaact ggttggtaat
    45361 aaaagcatca tactcacgct gcacaccaga aacgtagaca ccattgccaa cccagccgcc
    45421 agcgcccaac gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac
    45481 gttatagctg gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc
    45541 gctcatggcg ttattgatca agctggacat ggaggttcct tataagcctt caattacaga
    45601 gtattatcgg cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg
    45661 ctgtaggttt tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt
    45721 ttacgggcat agtgaggatc ggtagcatac cccgcgtcct gtagggcctg cgccccctgc
    45781 tccgcactcg cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg
    45841 taatccgaca atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc
    45901 gcttcgccgt tttcatattc agtcgtggtg atttcagtaa ctggcccttt ccagttgcca
    45961 gaggctttga caccaaacag gttatagctc ggctcgccgt tttcgcggcg gatttgccgt
    46021 tgcccccaac cagattccag tgccgcctga gcgaggatca aatgatgtgg cacaccgctt
    46081 tgctggcttg ccagttgggc gggcagcgag agttgcgcga ggaatgcttt actgtcaccc
    46141 ggcagcgaat catcgtagtt acgtggcacg gccttttgca ccagctgcga aagcgcctga
    46201 ttttgataac gcaccacagt ttcgagcggg actttcatcg gtgctgctgg cgtagactcc
    46261 tctggcaatg gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct
    46321 ttgcccgccg tcatctgttg ggcaatctgc tggtcataca tactggtata caggcgagtg
    46381 tgctcgctgc tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc
    46441 tgcacgaaca tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg
    46501 cccgctttcg cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt
    46561 ttgctgtcgc tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca
    46621 ttgattgcag tatggacatc agatccatcg gcgtagcgcc cagcgcattg agcgcgcgca
    46681 ccacgttatt gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta
    46741 aatcgatctg cgtttgtgga gtaaccacag tctgtccacc accaaacggt gtatctggct
    46801 ggctgacatt ggcctgacga ttaactgtta ctgagagatt cccctgcgct accgcgcagc
    46861 tgtcgagggt cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt
    46921 tagcgtcctg cggggtgaca ttaacatgca tattctgaat atcggcaagg aagcggacct
    46981 gggaactgtt gccactcggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc
    47041 tgccatatcc acgcacgcgg ttgatggtat cagcgatttg ctgcgccatg ctgaaatctt
    47101 cgtcgttaag ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt
    47161 caataaccgc accattggtg atccgtccac cgttcagttg gttaacctga acactgctac
    47221 cgccagcgga ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct
    47281 gactgtcaac gcccttaagc ggtgtcatca acaacgtacc tccacgcaag cttttggcat
    47341 tacccatgga agaaaccacc acatcgatgg tttgcccctg acgtccgaac ggaggaagtg
    47401 acgctgtcac cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg
    47461 taattcccag ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg
    47521 tctggtcacc ggtgccatcc agccccacca ccagaccata gccaatcagt gagttttgcc
    47581 ttaccccctg aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtgacca
    47641 gtagaagaat taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag
    47701 gttaaggaag aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc
    47761 tacgtattca atgcgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat
    47821 agtgcgtgga ttaaccacgc cagagaagcg aataaattcg gtaccctgat taatggcaat
    47881 ctgtttttca cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt
    47941 caacgtgccg ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc
    48001 accggaggct tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac
    48061 agtatcaaag ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct
    48121 ggcgctgacg ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg
    48181 atcttcaaac agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc
    48241 gttggcgacg ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg
    48301 cgtggagggt atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata
    48361 agtatgcgca gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc
    48421 tgccaccggt taagccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca
    48481 ccgctttact gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg
    48541 ccacgttgac gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc
    48601 ccggcgtgct ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa
    48661 tgctctccag cccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg
    48721 gagctgcctg gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta
    48781 acgcattcgc cggaatggtg atcgctggct gtacctgaaa accaccagcc gtcaccagct
    48841 gcccgttctg atccacctgg aaagagccgt cacgggtata ggctgatgag ccatctggca
    48901 acatcacctg gaaaaagccc tgccctttaa tcgcgacatc tttgctgttg ttggtctgcg
    48961 acaggtttcc ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt
    49021 gtaatccgga gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt
    49081 gataaagcag atcttcaaac accgcgcgct gacgcttata accgttagta ctgacgtttg
    49141 ccaggttgtt ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga
    49201 tccataatga actgatcata aaatgtcctg tagtggatta gtgacaaagt gcatatgtct
    49261 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa gtgctcgcaa aatcgataaa
    49321 ttgcgattgc ccccatagac ccgataaact tgcgcatcgg gcaacttata cttgtcatgt
    49381 atcctttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc
    49441 tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg
    49501 ccgcaacggc attgacgtta ctgccttcca gaacccccga catcacacgc aaggttggat
    49561 ctgcctgcag tatcggccca cgcgtggcct ggctttctgc gcttaaacga aaaatgccgt
    49621 cgtcaccgcg ttgcacttcg ctgcccgtgg ctttcaccag tttcagacgc cccactggcg
    49681 caaccgtatt tgccggatcg cccggattga gcgccgagat tgtgccatcg gcagcaatag
    49741 tgatttccgc cccttccggc acagcaattg gccccgcctc gcctatcacc ggatgcccct
    49801 gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta tacccttcgc
    49861 tgccgtcagc ggtctgcacg gccagccagc catcctgctg caacgcaacg tccagcgggc
    49921 gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac gccgttacca
    49981 gcgtgcgcgt aggcagtgaa agcccttcca ccggcactgc acgcaaggcg tttaactgcg
    50041 cgcgaaaacc gggcgttgag gcattggcca gattactggc ggttaccgcc tgttgattca
    50101 gcgtctggct ggctgctccc atcgcggtat aaattgcgtg atccattgag ctatcccgtc
    50161 agcgattagc gtaagttaac cagcgtgttg aggatctggt cctgggtttt gatggtctgg
    50221 gcgttagact gatagttacg ctgggcaacg atcatattga ccagttcttt actgagatcg
    50281 acgttggacg cttccagcgc accgttggtc agggtgccaa agttgcccgt cccggctgtc
    50341 cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc
    50401 agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg ggtttgttcg
    50461 ttggaatagt tgccgacaac cgtaccgtca tcattgattt gataactcac cagatcgccc
    50521 ggtttgtagc cattctgggt ggttgccaca atgttgttag cgccggtatt ttgctgcatg
    50581 gagttgagga agctcaggct aaacgtggcg ggttctgcgc cgttaattgc gccggttgcg
    50641 atattattcg ccatcgcacc atccactaat gtgccattag cattaaattt cagtgttgtc
    50701 gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg ccagttatta
    50761 tccccggtct tcacaaagta gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    50821 gtcaccgaac cttttttgtt atagctatcc gcattgctgg catcaaatgc gttaacagag
    50881 ggaagcggat cgctggaatt caggttgatc tgcatcgacg ccgtagtggt agttttcgct
    50941 gccatcaggg tattcgggat cgaaatatta gtcggattcg ccccttgctg aatagtcggc
    51001 ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt gcatattcac caggttacgg
    51061 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctatctacc
    51121 agacggaaaa aaccgttctg gctgatagca acgtccagac cgcgcccggt gttggtggtc
    51181 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    51241 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    51301 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    51361 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    51421 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgttgtt gccgcggatc
    51481 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca attgtgtacc accgttactg
    51541 gcgctaatcg ctacgttgta agaaccgttc ggcgcagtgc tgccgtcagt caacgtaccg
    51601 tcccaagtga aactgtggac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    51661 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    51721 ccaaacggcg tggtcgtggt cacagcccct tcttcactac cggttcccgc aagaacagtg
    51781 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    51841 tcaatctgtc cggaaataga gccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    51901 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    51961 tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg
    52021 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtg
//
LOCUS       DACAPB010000006        45455 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.006, whole genome shotgun sequence.
ACCESSION   DACAPB010000006 DACAPB010000000
VERSION     DACAPB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.006"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            118..246
                     /locus_tag="IT904_000319"
                     /pseudo
     CDS             118..246
                     /locus_tag="IT904_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
     gene            251..1282
                     /locus_tag="IT904_000320"
     CDS             251..1282
                     /locus_tag="IT904_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation (TAT) pathway signal
                     sequence domain protein"
                     /protein_id="HAP3190157.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSA
                     QATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYR
                     KELLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKA
                     RGLHDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRV
                     QGTEGLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDW
                     FVIHAFVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRK
                     PIFALNDAY"
     gene            complement(1293..1931)
                     /locus_tag="IT904_000321"
     CDS             complement(1293..1931)
                     /locus_tag="IT904_000321"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HAP3190158.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVA
                     GALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKP
                     VWKALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALP
                     WGLAGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
     gene            complement(1936..2301)
                     /gene="ble"
                     /locus_tag="IT904_000322"
     CDS             complement(1936..2301)
                     /gene="ble"
                     /locus_tag="IT904_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein Ble-MBL"
                     /protein_id="HAP3190159.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     gene            complement(2305..3117)
                     /locus_tag="IT904_000323"
     CDS             complement(2305..3117)
                     /locus_tag="IT904_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase NDM-5"
                     /protein_id="HAP3190160.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     gene            3316..4332
                     /locus_tag="IT904_000324"
     CDS             3316..4332
                     /locus_tag="IT904_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HAP3190161.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
     gene            complement(4441..5427)
                     /locus_tag="IT904_000325"
     CDS             complement(4441..5427)
                     /locus_tag="IT904_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP3190162.1"
                     /translation="MSYHHLNFEDRTALMLESRKEGFSARKFAELIKRHPSTIYRELK
                     RNSINDVYQARYASDNTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTF
                     PDLDQTMNVSHTTIYSTIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKT
                     IHERPAEVQERKIPGHWEADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKA
                     LTEALKYLPAELRKTLTYDRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGL
                     IRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPLGKVRISGEILLG"
     gene            complement(5662..8679)
                     /locus_tag="IT904_000326"
     CDS             complement(5662..8679)
                     /locus_tag="IT904_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /protein_id="HAP3190163.1"
                     /translation="MAFEERVQILSEAEQDELYGPPAFTSADQRFFFSLNDKELAIAK
                     SLRHRGQRYMLVVLLGYFKAKPVVLNPGFHQIKQDLKYVYQTVLPGPGCRPFNLTPKE
                     NERIYQRVFQLCNYQRWNVKDHGAALRDYLSQQARAWTAPRHLFDAANEYCSGQKIAI
                     PAYSTLQKIISQVVGDEQEHMAAHLERAMSRGLKQALAELVNGTGPLPFRQLRQSARN
                     FTGTELEKELIVYRHIQHWMPEVDLLLSTLSLSQKNLQHLAEKVDYYGAKLKRQTVGS
                     QWLYLLCYLQTRWQQALERIADGFVHHVRQTKQKAKDYAQEAVFKDWQKAAKNVSKAA
                     EVLHLFIDDSIDLQLPFATVRQQALSLLTKRDLESVCLFLNEQRRSVDEAMWQYCDEK
                     ESLRKGLLRELFLCLRFEGCDGTQHLAAALAKTQNELNGQDAQLQTADTRLLSKKSRE
                     FLLDGEGNILIDRYEWFLYQQIPDRLNGQLTLPDITKYRALDADLIDGEHWRKNKYTL
                     LQQSHFTKLAEEPEKLIKQMAMELDTRLYEVGEYLEQEDNRNIILRNPQGKRFWRLPS
                     ASKHHLVNNPFFQQIPTTGIADVLRMVDRDTGFIDCFAHVLGSQSRSRSHEYDLLAIL
                     VGNATNQGIYGMAQISDRTYDQLSTIQANYLRLETLNAANDNINNATAKLPIFRYYNI
                     QEDVIHASADGQKFEARRETFKTRYSSKYFGTQKGVSAMTLIANHAAINARVIGANEH
                     ESHYIFDLLMSNTSDIIPDVLSTDTHGVNHVNFALLDLFGYQFAPRYAQVGKVINDMF
                     DVKEDKEHRIQLCLKKPINTHRIAQHWDTIQRIAVSLKQRKTTQATLVRKLSEYKRNH
                     PLLEALTEYNRLVKANYLLCYIDDASLRNYVQRALNRGEAYHQLRRAVSSVNGDQFRG
                     SSDEEIQLWNECARLVTNAIVYFNSRILSQLLTSFEYQGDTKRIDIVKQASPVAWHNI
                     NLKGTYHFELSEKLPDLEELMRSIEGYLPVSEK"
     gene            8823..9368
                     /locus_tag="IT904_000327"
                     /pseudo
     CDS             8823..9368
                     /locus_tag="IT904_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10344..10529)
                     /locus_tag="IT904_000328"
     CDS             complement(10344..10529)
                     /locus_tag="IT904_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190164.1"
                     /translation="MDRKSNSDSYQRWMIEKADKRERTQISMSFVKMKDVLFNVLAIG
                     TVLAALVVIFYEVQSSL"
     gene            complement(10487..10699)
                     /locus_tag="IT904_000329"
     CDS             complement(10487..10699)
                     /locus_tag="IT904_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190165.1"
                     /translation="MVTQSLADFGRYSYKPRPAFQDWIERLKTGERLPRGRYFVGKKP
                     GGRLVLIDELGGTWTEKVTVIHTNAG"
     gene            complement(10762..11037)
                     /locus_tag="IT904_000330"
     CDS             complement(10762..11037)
                     /locus_tag="IT904_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190166.1"
                     /translation="MHTTNAIILCLVSYPCIVAWVIYKNRFVDYLFLTLLACLGSIFS
                     VVLYKQPDYSLTIYQAVAVGYAAVIMPTVLVLSVVNIFMGKAERTCK"
     gene            complement(11055..11309)
                     /locus_tag="IT904_000331"
     CDS             complement(11055..11309)
                     /locus_tag="IT904_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190167.1"
                     /translation="MGHYTIRTNDDEDQAIKKAQEATGQASASKTFMTAILELQRNRD
                     EMAQLRRELAQEKARSQELVSSVKQFRSSLNNLFDLADNP"
     gene            complement(11419..12240)
                     /locus_tag="IT904_000332"
     CDS             complement(11419..12240)
                     /locus_tag="IT904_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAP3190168.1"
                     /translation="MTRATERAYSELQQAFDFYNQRLFDGELPDCLITFQRGKNTMGY
                     FSYRRFVAADGSGRMIDEIALNPEYFPVYPLIEVMQTLVHEQCHMWQYHYGNPSRKTY
                     HNAQWAAKMESIGLMPSSTGRPGGAKVGQKINDYPIPGGRFQRVTLELFQGQFALSWF
                     DRFPVQVEQQKDMTAVIEQWRETLALAHQNAEAGIDIEAVLSMALLPSVSPQNGSHSD
                     DMAGSNDNDLSVAAFEDKPKRNKVKYQCRGCGAAVWGKAGLNIECGDCELAFIEN"
     gene            complement(12237..12416)
                     /locus_tag="IT904_000333"
     CDS             complement(12237..12416)
                     /locus_tag="IT904_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190169.1"
                     /translation="MTEKITDEELVDLLEALKRAHGMGVCSKAVKLAQRCADVFPAIV
                     AELQEYRNAAKRTSA"
     gene            complement(12433..12699)
                     /locus_tag="IT904_000334"
     CDS             complement(12433..12699)
                     /locus_tag="IT904_000334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190170.1"
                     /translation="MISIAFASYILLALVVHLTLKQASYNGKVSSPAVNHACQQTSFL
                     QPVWPLIFASNITRSMPALGVKLPAKKIAAIKGVVYIICIVRYR"
     gene            12689..13339
                     /locus_tag="IT904_000335"
     CDS             12689..13339
                     /locus_tag="IT904_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP3190171.1"
                     /translation="MDIKPNSVIHLEPGETLPGYTNLKPVPDKYFDELKALINKLNTQ
                     SGNIYLKLKQMYAFLDRFNKEFVSTFTSCQKGCSSCCKMDVHLTALEATHIAQASKLT
                     ARDNPLTTGHESKCPFLSEKGTCSIYNYRPLLCRTYHVLTPPEMCNDLDAQVMQYGSQ
                     SANMGNHIYKTIAEWIYFQTYHCTGKLETKDIRDYFPYPREDIQRFLHHNPPRPFC"
     gene            complement(13377..13832)
                     /locus_tag="IT904_000336"
     CDS             complement(13377..13832)
                     /locus_tag="IT904_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAP3190172.1"
                     /translation="MSENETFDATKKLLLNIRSVRVFARETSFEQLLEMQEKLNAVIE
                     ERREEAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEDAQKTRKKTLPKAPPK
                     YQFDENGETKYWSGRGRTPKPIDEALKAGRSLDEFLIKKDASSTAGDEQ"
     gene            complement(13844..16171)
                     /gene="topB"
                     /locus_tag="IT904_000337"
     CDS             complement(13844..16171)
                     /gene="topB"
                     /locus_tag="IT904_000337"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP3190173.1"
                     /translation="MRLFIAEKPSLAKAIFEGLGGNPNTEKKNGYFEHGSDVVTWCYG
                     HMLELYDPQDYDDKYSNWRYSDLPIKAVYPPKYKIKPESEAQTRIILSLIDKADSIVH
                     SGDPDDEGCLLVDEILGYAGNKKPVQRLLVADLNLAPVQKSLSNLQPNDKFRGMTLSA
                     LARSLCDQGFGYNMTRGCTLKGREKGYDGVLNVGRVQSAVLGLVNMRTLANQNHTESF
                     YYDVFASLSMNGHQIKAKYQTTDDDQIDEKKRLISEAQAAHIAGRVTGKDAIVTIATT
                     KPENSKPPLPLNLSTLQQLCAKRFGYSAKDTLDVMQGLYETHKLLTYPRSDNRYLSDE
                     HYYQAGDIATAIAATVPELASAIVDMDKGQKHKAFNASKIEAHHAIIPTTKSGAGIQL
                     NEKERNVYNLVSVYFIGLFYPDAIRNKTKIHFDIKGDTFTATQSVLVQKGWEALGKDS
                     DDEDETEDAGTDGFDLSLLKFNDSGLCESADVDKKKTTPPRYFTESTLLAAMTSAAKF
                     IDDPALRKALEAKDEGSSDRGSIGTEATRAGILEKLAANTGLISIEKEKGYSELVWKT
                     TKQGQEFCAALPPEITKPDISALWAEKQSQIKAGELTVEEFIKENDEYVQGLIDELDR
                     NGISISSNATPCPVCNNGFLRKRKGQNGFFWGCSCYPECKTTFPDKDGKPDMEAKSRS
                     EGSMSRLEAPCPSCSKEIIIRPKGFFCSGCEFKIWSEVSGKKLTQNQVETLIKKGKTG
                     EIKGFTSNKTGKKFDAAIVLQDKTTGKLGFQFSKK"
     gene            complement(16175..17437)
                     /locus_tag="IT904_000338"
     CDS             complement(16175..17437)
                     /locus_tag="IT904_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP3190174.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRK
                     NRKIYSNLDIEPVASTVVHTGSSPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIR
                     FILNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKIGCYDGVYYHGKAR
                     KEEIENALRFSVHPKFNELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIA
                     KALGAKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGA
                     DTPRNLAGTYVIFSTNYPNRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPDDI
                     NIDLKELGAVLDRTTPAEIKEIINIAIGMIRGTKNELTVDVIKNARAFLKGTLDLSVQ
                     EAEEDIEEREEIFKKNGAQPDYSSYLED"
     gene            complement(17520..18014)
                     /locus_tag="IT904_000339"
     CDS             complement(17520..18014)
                     /locus_tag="IT904_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAP3190175.1"
                     /translation="MRHFFNVLSFAVALLPATLCAAQIQGKVIRVLDGDTIEVKTLPA
                     KIVVYEVPIRVRLINIDAPEKKQPFGRWSTNQLKALLAGQSVTVSYTQTDRYGRVLGR
                     VVTANGTEANRQQVLKGAAWVYDRYNTDNSLPALQREAQTQKRGLWADSNPVPPWEWR
                     HKQN"
     gene            complement(18011..18337)
                     /locus_tag="IT904_000340"
     CDS             complement(18011..18337)
                     /locus_tag="IT904_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190176.1"
                     /translation="MKIINVPDARSVRIAGIEIIDSVKSVASVFTEILLFMVWFPAIV
                     YGLYRFVIVLWDASLKPQSLIEFINFMVELYSYVLPSLAVIAVALVVVLSIFKRLSAI
                     WEGRKQ"
     gene            complement(18423..18737)
                     /locus_tag="IT904_000341"
     CDS             complement(18423..18737)
                     /locus_tag="IT904_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190177.1"
                     /translation="MKKTVTTAVLLCAFAAGTAHAEEKADPNDPCAMVLCLAGKLDGS
                     SPAECDPMYKSFMSIRKKNKHGFLPDHTADARKKKLNECPAADAGTVSKIISSFGRLK
                     NF"
     gene            complement(18825..19217)
                     /locus_tag="IT904_000342"
     CDS             complement(18825..19217)
                     /locus_tag="IT904_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP3190178.1"
                     /translation="MKKMLMVSVLFLSACSSPPEPPQVDWEQNPETVNTQLMDWQPTY
                     SVIKSDKVNSSWVKVIHNFRPENRLYDDAVFYSVAHSDSVIVETSNGTDFFTAKNWLR
                     ANGANGVIQYRYKMNCFSCRTTSVYLSR"
     gene            complement(19214..21049)
                     /locus_tag="IT904_000343"
     CDS             complement(19214..21049)
                     /locus_tag="IT904_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP3190179.1"
                     /translation="MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKN
                     FDSMLLWRIITESNIRTDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKF
                     ASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPN
                     LLVRKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLFYIDLKEES
                     GAKDLLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKP
                     VFSIGSVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQR
                     SSIDGSFRKKMSLFYLPTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNL
                     FFNFVVEVTLRENPDFDPTLKHDCLMFLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIY
                     QNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKISEKLGYITTKSTGSSKTSG
                     RSTSKSSSESEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPYFMDRLM
                     MVSPKLTELTASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL"
     gene            complement(21052..22086)
                     /gene="virB11"
                     /locus_tag="IT904_000344"
     CDS             complement(21052..22086)
                     /gene="virB11"
                     /locus_tag="IT904_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAP3190180.1"
                     /translation="MKDAENRHIVYEIIDEYFHHWLNEVDGLTEIAVNRPDELFVKVS
                     GKWQQHELKMDFKDCMSFAGAISDYHDGGSVTPEYPLRSVTLPGGERVQIVIPPATER
                     ETVSITIRKPSSVFIDHDTFVKQGFYSRLNQGGKFRDKEDELSSMFKDNCFEQFVPEC
                     LVKGKTMVFCAGTGAGKTTFANACLQYIPHHLRCISIEDTDEAKFRFHKNHVKLYYPS
                     EGESSTVSSASLLRSGFRMNPDRILMTEVRGAEAWDFLKGSSSGHAGNLTTVHESTPE
                     DAVLGLVQRCYMNPECQNLPYNIILRRVLSNVDVIMSIKYIDEEDNRFASGIYYRDIH
                     FQEYFEKLKE"
     gene            complement(22083..22280)
                     /locus_tag="IT904_000345"
     CDS             complement(22083..22280)
                     /locus_tag="IT904_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP3190181.1"
                     /translation="MITLDIKEFSMLLGIRESEIYHHIRKGIPINGVPFPKPLKQIKT
                     HRFNYEEVMRFIEDLKGKGEL"
     gene            complement(22282..23496)
                     /locus_tag="IT904_000346"
     CDS             complement(22282..23496)
                     /locus_tag="IT904_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAP3190182.1"
                     /translation="MSEQENKIPTAVEIEQALRERHQKEIEQNDKTDKGDEDDGKSVK
                     RLGIEKLKKSRKGLVIIVAAFLLLAAGVSVYYIPSIIRAMSSGDDKPASNAVSTGSVK
                     RATGLSDDVDPFNTREEPAKTEERKESSGKAETPPEKVQQNFSRALDVAYGGSSSASS
                     NSGGSSSTASNTRNEGDGSDKQAEVQPVNAGQPAQLSKITRVPYDPNLFIPENTAIKC
                     SLDRRFISDLAGKLVCTINEDVYSANRNVKLIEKGTAAYLMYKTGTFNHGQGAVFIAA
                     TKLRTRKEPFIDIPLIDTQAAGALGEAGASGWIDTHFADRFMGAMMVGMIPDVAQAAS
                     GAAKSNKDNQTDYTANSRQAFADIAREAFANSVNIPPTLYKNQGEIITLIVGQDLDFS
                     SIYKLKMVGTRR"
     gene            complement(23493..24422)
                     /locus_tag="IT904_000347"
     CDS             complement(23493..24422)
                     /locus_tag="IT904_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAP3190183.1"
                     /translation="MKMNKGALIVALLLVSHVCHAAVTPAASRFDPRNQIVSYNPQNT
                     TVINSAVGYTTTLVFDEDETVISARTGFPQGWEVNKEDNLVYLEVKPVKQTVQKNSTD
                     DNGNQSSEVVSVALDPENDLERWRTNLFVRTTKRNYSMELNARTFRQPDKIAFVVNYQ
                     YPQERRKEQAEIEKKRIEALNKRQEEQAINRSLENAKSPKNWDYWKRVADGSQSISPD
                     YAYDDGRYTWFGFSPLKKIPSVFVMSGEQETLTNPVVKNSGSFTVVGVPVDQRFVLRM
                     GNQVVGVENRGFGKVRLPAGDTVSPNVEKEVIQ"
     gene            complement(24428..25156)
                     /locus_tag="IT904_000348"
     CDS             complement(24428..25156)
                     /locus_tag="IT904_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAP3190184.1"
                     /translation="MGFKLSGLKLPGFNKKKEVTDSSTTFEEKNVLMQEKMNRIYKFG
                     GIAGIAVGVLSLLALNGALPLKSTVVEAYLINGVTGVAERLTSVKKENLSENEALAKY
                     FITQYVKRREGYNYFSLQHDYDYVLLYSAENVATDYNALINGNQSPKVIYNKAEKTAS
                     VQDNPSVIISPSSRRDDKDMGAYIRFKLTIRNVATGQDDYEYWNVRLTYRIEPQVEMA
                     SGDRNNNPLKFVVTSYVRDKEVKG"
     gene            complement(25351..26406)
                     /locus_tag="IT904_000349"
     CDS             complement(25351..26406)
                     /locus_tag="IT904_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAP3190185.1"
                     /translation="MATSRYFQAAHDVIVKTLDQSLTGQLDNFSSIASQLGKYGISIY
                     ILWYAYTTIVGKQKTAVQDFLWNLCRFWLILVFVKNMGGWLDSATQAIDGLKETFAGG
                     DPWKWVDELWEKVQQVAAYLMSKDTSKYVKTDGAIASLLTYAGGIIALLLTSIVYLAA
                     EVTIKILTITAPLFIICLSFGFLRQMFNSWLQLIFSSCFIFLFCGLAIKAGMTFLNGI
                     LTISIANADELNLISTGAQAGVAGAFMAWIIWQAKTYASQLAGVGVEGAMQGAAAMGI
                     GAGVFGASRMGRNLLGMGKNAGIGAWKGVRRQDGGFNQSPGVSGKLGNLTGQGVNIGA
                     KRLRQAAIDAAKKKYGG"
     gene            complement(26418..26675)
                     /locus_tag="IT904_000350"
     CDS             complement(26418..26675)
                     /locus_tag="IT904_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAP3190186.1"
                     /translation="MKKYGLVAALGLTFFLTGCEEVKSVDWWQSHVDDATKKVAECKK
                     SGSDSENCKNAKEGLFRYKQLHPKHVDYKDAFKNVLGKDGS"
     gene            complement(26685..27455)
                     /locus_tag="IT904_000351"
     CDS             complement(26685..27455)
                     /locus_tag="IT904_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP3190187.1"
                     /translation="MKKHIVAALIASVTVISGAQAGVPVAIDANPEWAVEAQRWTERL
                     KQWQDTVNHYQKQINAYKQELLTKTGIRDVQGLVQSAQSVSKELENIYDQGNSFIDDY
                     IKNPEATLSEQARSLLADYKVTNTCKGLGYTGDLVRGCEASFLSQLAGIEYGNKLESK
                     LREDNQEMADLIDQVKNAQDTKATQDATNAVSLASLKFNKLKFQYEMYRDKQRDLAEY
                     KEKMLQAAFQEKQLGAVNKETPVVDYKAAFEQQSYEMN"
     gene            complement(27465..30218)
                     /locus_tag="IT904_000352"
     CDS             complement(27465..30218)
                     /locus_tag="IT904_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP3190188.1"
                     /translation="MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYA
                     LILPGWYAIKRVTKIDERFFDLLYLRMQIKGNPLANKRFNAVHYAGSSYDAVDISKVD
                     NFMKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQIDGAYFECVDEADLALLTDQ
                     LNTLIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSAAEYFENKL
                     YLTVCYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLY
                     EENNIVYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARY
                     FRCIEIKDYFQETDAGIFDALMYLPVEYVQTSSLTPIDKQSAIKALDDQIDKLEMTDD
                     AAKSLLADLKVGLDMVSSGYISFGKSHQTLIVYADSPERLVKDTNIVTTTLEDLGLIV
                     TYSTLSLGAAYFAQLPGNYTLRPRLSSISSLNFAEMESFHNFFTGKEKGNTWGNNLIT
                     LGGSGNDIYNLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFAAQQFGT
                     PESFPPERKLKKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNI
                     SFMKDLVRLLCTLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTET
                     RRHGLKARLNAWVQGGEFGWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAASFYL
                     IYRVTMLADGRRLLIYMDEFWQWINNEAFRDFVYNKLKTARKLDMVLVVATQSPDELI
                     KSPIAAAVREQCATHIYLANPKAKRSEYVDELEVRELYFDKIKAIDPLSRQFLVVKNP
                     QRKGESDDFAAFARLDLGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTYLERA
                     NLI"
     gene            complement(30243..30533)
                     /locus_tag="IT904_000353"
     CDS             complement(30243..30533)
                     /locus_tag="IT904_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAP3190189.1"
                     /translation="MLKLNKRYLTLSVFMAALMLCVAEPAFADDVSTKTTGFLQKIID
                     FLTDIRKPAITIIALVIGYIAIFSRQHAAWITPLIIGIIIFIVAPYLPDWLA"
     gene            complement(30517..31161)
                     /locus_tag="IT904_000354"
     CDS             complement(30517..31161)
                     /locus_tag="IT904_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAP3190190.1"
                     /translation="MLSTTAFAALALQCAASVHPDTAHEVARVESGFNPYAIAEIIPK
                     VERKPGDKGVVSYFPKTKEAALQIVNQIESRNHRYSVGLMQITSTNFANFNTTAEKMF
                     DPCENLKVSEQILVDCYKRGGDILRGLSCYYSGNPETGTKPESDFNNTSYIQRIGFNP
                     PDNKKNWVVPSVKDAIRKENVTQSIKPKEVTVYPQYAMRGTVLDEKETNDVKTQ"
     gene            complement(31394..31903)
                     /locus_tag="IT904_000355"
     CDS             complement(31394..31903)
                     /locus_tag="IT904_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HAP3190191.1"
                     /translation="MEWYVLQFTTTRFAAVFAHLERLNFSYFCPMETERYRRPDKIIS
                     YRERRLPLFPGYLFIQADFEEVHSTTITAIPYVQRFISFGGEPLPVPEDVMAELLYRQ
                     SHTTAQANLLRKSIPHDFAEILLMDNPQQRSMAFIHYITERSLTHKMKRKKNDCYPKK
                     NRNETQAPT"
     gene            complement(32256..33416)
                     /locus_tag="IT904_000356"
     CDS             complement(32256..33416)
                     /locus_tag="IT904_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP3190192.1"
                     /translation="MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERIN
                     RKGASKEVVVKISGGAITRQGVRNSIDYMSRESELPVMSESGQVWKGDEIQEAKEHMI
                     DRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPEDLLESVRKTMNKKYPNHRFVL
                     GYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYDVKATHKQQ
                     HGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYW
                     GADFGELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGV
                     KGIDRISSSSKELVLNSAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG"
     gene            complement(33419..33964)
                     /locus_tag="IT904_000357"
     CDS             complement(33419..33964)
                     /locus_tag="IT904_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HAP3190193.1"
                     /translation="MKKVQFRIEESQHDDLLDCLKTLYPDEPGLTVAKGMKLLANALL
                     KSKVKNEDVNVSKDNDDFIKTTMYLTGKQRTLIEKAAHRHGWNLSRECRYRIQTTLEN
                     ELDFFDQELLVMNRCRNAIDKIGRNFHYIIVNDNARVLDKDGFYQDAERLSSEIMSLK
                     SEFENYIMLCKGRTVSNKVEV"
     gene            complement(34305..34562)
                     /locus_tag="IT904_000358"
     CDS             complement(34305..34562)
                     /locus_tag="IT904_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190194.1"
                     /translation="MKKQEFLDFISAEQRRGAVRFSLGFNSKGEIVLHWTNEAGLRVW
                     SILSGNRGKSPSRANRERMSNLRRWLHDARQGMEGDTPEAE"
     gene            complement(34798..35136)
                     /locus_tag="IT904_000359"
     CDS             complement(34798..35136)
                     /locus_tag="IT904_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190195.1"
                     /translation="MNTYVITFQALNTKKEKITCSAFVHSETLFLAVHSFEDKNRGRG
                     YVITSVSELLSEELTAKNSLKKNINFWFNECGLSKSEVINEVINWKNFAYTLKELEEA
                     KNEAIRELRS"
     gene            complement(35230..35472)
                     /locus_tag="IT904_000360"
     CDS             complement(35230..35472)
                     /locus_tag="IT904_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190196.1"
                     /translation="MSFKPTPEEVKQARIKAGFTQQEAAERFGFTLSAWQAKETSGKT
                     SRGLAAGTYELLLLLADEHPDYQLVKREKNQDKVTK"
     gene            complement(35462..35674)
                     /locus_tag="IT904_000361"
     CDS             complement(35462..35674)
                     /locus_tag="IT904_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190197.1"
                     /translation="MSEDATRLFIELEKLMKVRSKEADKKTTKKIVFQKSFYYEEFSN
                     VKDEIDDPILLKFYTDTIVKVQNHEF"
     gene            complement(35740..36291)
                     /locus_tag="IT904_000362"
     CDS             complement(35740..36291)
                     /locus_tag="IT904_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190198.1"
                     /translation="MKEQKNFFERYKPVFEIVCRILGNGWRVNLLDDCQYKIKLTSPD
                     FKNYSIHIRMEKGRLVIIGSVDSRSWRSPYHTCTVSPERNPVEIAADIEKKILSDALD
                     NVDMAREYEQQLQQKREKKLILKGMLSRLVHLESWHGTLTGFKVENGLDGNVSERGDG
                     YEMVIRGLSVDQLIKVAGFIKQL"
     gene            complement(36351..36587)
                     /locus_tag="IT904_000363"
     CDS             complement(36351..36587)
                     /locus_tag="IT904_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190199.1"
                     /translation="MKNKTITEAELINIFESYGAYICPDEIEVTAKECNENGSVLHRG
                     LNAEGWAHLFAKEEAYQQECEAQEAASDDGHFDE"
     gene            complement(36646..37161)
                     /locus_tag="IT904_000364"
     CDS             complement(36646..37161)
                     /locus_tag="IT904_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HAP3190200.1"
                     /translation="MNIQEALNVFGLSGDLTEKDIKAAYKKAALKYHPDRNPLGAELM
                     KAVNAAFDFLMANIDKINQFQSTDENARYNYGEDLEKVLNTLSGLTGIVYEVIGNWVW
                     ISGETKEHKDILKEMGCKWASKKKQWFYRPEEHKSRWNRKEHSIEEIREMYGTAGKRK
                     ASGWTRVEASA"
     gene            37415..37573
                     /locus_tag="IT904_000365"
     CDS             37415..37573
                     /locus_tag="IT904_000365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190201.1"
                     /translation="MRAITPLTPARPISDYQTMFSLSQLARELANGTPGQCETLARGA
                     VSARYEMR"
     gene            complement(37751..37903)
                     /locus_tag="IT904_000366"
                     /pseudo
     CDS             complement(37751..37903)
                     /locus_tag="IT904_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            38514..39527
                     /locus_tag="IT904_000367"
     CDS             38514..39527
                     /locus_tag="IT904_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP3190202.1"
                     /translation="MRLLLSLDMKILRSSYLKVRGFLIKGCIVVLNYNLCGVMICLHR
                     KGYMRLIVKTVTGLTKVRHRNEVGVTLASLSLSAKRVLFLALCQIDTKEMLDDDILEV
                     DADFFSKATSLDKYASYAALKEGAKVLSSTTLVLNRDDLKNLADELGILSSKNKIPDR
                     LDLNLTEFCAYYDHLATVRIKFTNTAKRYFSKLIGSENRYTTQVLKSVVLLNSVNSTN
                     LYQVIRKYYSQNSSKKSFDISVDDLKEEMGLYTIEEGEKKYKYPKYSFFVRDVINKSI
                     NEIIEKTEINQLSFSVVGKKGRMAHMLRFEFSINEKSSSFSEDDMAFLEEFDKVVPPK
                     KNK"
     gene            39536..39979
                     /locus_tag="IT904_000368"
     CDS             39536..39979
                     /locus_tag="IT904_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190203.1"
                     /translation="MAKIYEFPQGAERSKLKKEIIRERKKRLRETNGNPVIRHAKWFW
                     FYLRLATAGALHLVSVISLAVLGAFSKAIFWIGGMLCVVTWFHLERQFWTPQNFTIPV
                     IVTLWGLSLFATPLMELLNKKMPWYRLLVPDAKHTSTEATNDDQP"
     gene            40167..40520
                     /locus_tag="IT904_000369"
     CDS             40167..40520
                     /locus_tag="IT904_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="HAP3190204.1"
                     /translation="MKYQVKEFINEKYSKAVNILKDNLKEHYHIFYGLRLSEILFPAS
                     EYGSEMFFQEFEAINSVILPLVIFDLIDRKPIMVIGFGEVCGVDSLVDSGIEVVSLDG
                     LSDLLLVEKLTPLFN"
     gene            complement(40541..40825)
                     /locus_tag="IT904_000370"
     CDS             complement(40541..40825)
                     /locus_tag="IT904_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190205.1"
                     /translation="MNKSWLEQQVQNEKLKKVQEEKKDGTIQKGLYRVRIKDKYRWDL
                     WENDFRVVIAYSKDDAELKFTEMTGFNEHYYIESIEKIVLSDIEKILLEN"
     gene            complement(40861..41172)
                     /locus_tag="IT904_000371"
     CDS             complement(40861..41172)
                     /locus_tag="IT904_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190206.1"
                     /translation="MFIFRYIKTLINIYLLYIQPILGMFLIGIGLWGFYAPDEGTCTN
                     LNIELCAVSAIDVLKPAIFVFLGIITVAGWYFTELQPKLRGEGKYMKVKTGVKPEDFV
                     K"
     gene            complement(41378..41662)
                     /locus_tag="IT904_000372"
     CDS             complement(41378..41662)
                     /locus_tag="IT904_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAP3190207.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVAGKPKLMNFR
                     LPAYFEEILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            complement(41762..42424)
                     /locus_tag="IT904_000373"
     CDS             complement(41762..42424)
                     /locus_tag="IT904_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="HAP3190208.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     IL"
     gene            42805..43467
                     /locus_tag="IT904_000374"
     CDS             42805..43467
                     /locus_tag="IT904_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3190209.1"
                     /translation="MTQTVPKGFVRAYLRASTTEQDATRALDTINTFAIERGLSICNY
                     YIENESGSKLERPELFRLLKDCQQNDILLVEDVDRLSRLVGEDWNTLKKMIRQKDIRV
                     VAVNVPTTWLASGHNDFDSRMFSAINDMLLDMLAAVARRDYEQRRERQQQGIARAKRE
                     GKYKGRQVNQSRYDAINRLIASGSSWSQIQKVLGCSRATISKAVKQQTVNKMNHEPEG
                     RE"
     gene            complement(43556..44776)
                     /locus_tag="IT904_000375"
     CDS             complement(43556..44776)
                     /locus_tag="IT904_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
                     /protein_id="HAP3190210.1"
                     /translation="MDEKSLYAHILNLSDPWQVKSLSLDENAGSVTVTIEIAENTRLA
                     CPTCGKSCSVHDHRHRKWRHLDTCQFTTIVEADVPRIMCPEHGCLTLPVPWAGPGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLARIKKPLSARHMNVD
                     EVAFKKGHRYITVISDRDGRALALTDDRGTESLAGYLRTLTDGQLLAIKTLSMDMNAG
                     YIRAARIHLPSAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRHQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLRAQMKLTSQCWALKELAKDIWNRPWSEERRSDWQRWLALAA
                     NSDVPMMKNAAKTIGKRLYGILNAMRHSVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAF"
     gene            44878..45117
                     /locus_tag="IT904_000376"
     CDS             44878..45117
                     /locus_tag="IT904_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V"
                     /protein_id="HAP3190211.1"
                     /translation="MPRHYEIDSAWRASIKREPNGRQTVTTEAFVSQLALINFHWSCR
                     QANQWIETYVTVFKDISTQEGENRTFMLFNPNGGR"
BASE COUNT        11511 a        11093 c        10067 g        12784 t
ORIGIN      
        1 acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc
       61 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgcccccc
      121 gccctgcagg aacgcctgcg ccaactgcat ccctacgaac tcccggagct gctcgcggtc
      181 gaagccgcgt ccggcctgcc cgaatacctg caatggctgg ccgccgagag ccgaccggta
      241 aactgagcca atgaccgcat ccacgatccg cctgcgccgc tggctggccg ggctcgccct
      301 gctgcttgcg ctgcccgcga cctcggccgt cgcccaggac ttcgaactgc cgccggtcga
      361 cgaggtcttc gtcctgtccg cgcaggccac cgcgccggac cgcatcgagg tgcgctggcg
      421 gatcgccgac ggctattacc tgtaccggca ccgcacctcg gtcaaggccg atgccgcctt
      481 caccggcgcg accatggcgc tgccgaaggg caaggcctac cgcgacgaat tcttcggcga
      541 cgtcgaaacc taccgcaagg aattgctcgg caccctcacc ggcacgcccg cggccggcgc
      601 gagcgcgacc accctgaccg tgaagtacca gggctgcgcc gatgccggcg tgtgctaccc
      661 gccgcagacc cgcaccctga aggtcgcgtt gccgggcgaa gcgggcgctg gcggcttcgg
      721 ctgtaaggcg cgcgggctgc atgattatgt cgtcaagaac ggctctgccg atcatcccaa
      781 cgccgaggtg aaattcgcac tgggtgatgt ggtcaacacc atgatcggct gcactaatgg
      841 tgaaacgatc atgctgtgcc acgacacctc gctgccgcgc ccctattctc tcggctttcg
      901 ggtgcaaggc accgaggggc tgtggatgga cgtcaacaag tcgatctatc tggagggcaa
      961 gagcccacag ccgcaccgct gggagcctgc cgagggctgg tttgcgaaat acgatcaccc
     1021 gctatggaaa cgctacgccg atctggcggc aggggccggg catggcggga tggactggtt
     1081 cgtgatccac gcttttgtcg aggcgctgaa ggccaaggcc ccgatgccaa tcgacattta
     1141 cgacgcgctg gcctggagcg cgatcacgcc tctgtcggaa caatcgattg ctgagggcaa
     1201 tcgcacgtta gattttcccg acttcacccg agggcagtgg cgcacccgca agccgatctt
     1261 tgcgctgaac gacgcctatt gatcgacgcg atttaggcca agcgcaccgc aaaggcgaaa
     1321 ttggtaatct tgtcggtatc cttgacgccc ggcgcgcttt cgacgccgct ggaggtatcg
     1381 accagcggcg ctccggtgcg cgcaatcgcc tcggcaacat tcgtcggatt tagcccgcct
     1441 gccagccccc acggcaaggc accgcgatat ccggccagca gcgaccagtc gaacgccaac
     1501 cccatgccgc cgggcagcgc gcctttgggg gtcttggcgt cgaacaagat caagtccgcc
     1561 gccccggcat aggctgcggc gcgtgcgaca tcgctggcgc tggcgacggg cagcgccttc
     1621 cacaccggct tgccaaaccg cgcgcgcaac tgggccacgc gttcgggcga ttccgaaccg
     1681 tgcagctgca gcgcgttcag cttggctgcc accagtgcgt cggcgatgac agcatcatcc
     1741 gcatcgacga acaaaccgac catggcgatc tggccagctg cgcgcgatgt caaagcgccc
     1801 gcgacattcg acgtaaccgc acggggcgac gctggataga acaccaaccc ggcatagtcc
     1861 gcccgcgccg cgatggtcgc atcgagcgcc tcgggtgtgc tgatcccgca aatcttgatt
     1921 ttcgcgggca tgcggtcagt cggggttctg gatcagccgc accagcgtgc agtcgggatc
     1981 gatcaggtag ccgatcctca ggccgctcgc ctccagttgc ggagctttga agcgcggcca
     2041 gccggtgctt ttttcctcgg ctcccgccgc gttcaccaat gccaccatgg catcgagatc
     2101 atccaaccgc aggcaacagc cgaacgagct cgtagctggg tcgaggtcag gataggggaa
     2161 gaattcgagc tgcaaaccgc cgcgctgcag gatcatccag ccgcgatcct tccaactcgt
     2221 cgcaaagccc agcttcgcat aaaacgcctc tgtcacatcg aaatcgcgcg atggcagatt
     2281 gggggtgacg tggtcagcca tggctcagcg cagcttgtcg gccatgcggg ccgtatgagt
     2341 gattgcggcg cggctatcgg gggcggaatg gctcatcacg atcatgctgg ccttggggaa
     2401 cgccgcacca aacgcgcgcg ctgacgcggc gtagtgctca gtgtcggcat caccgagatt
     2461 gccgagcgac ttggccttgc tgtccttgat caggcagcca ccaaaagcga tgtcggtgcc
     2521 gtcgatccca acggtgatat tgtcactggt gtggccgggg ccggggtaaa ataccttgag
     2581 cgggccaaag ttgggcgcgg ttgctggttc gacccagcca ttggcggcga aagtcaggct
     2641 gtgttgcgcc gcaaccagcc cctcttgcgg ggcaagctgg ttcgacaacg cattggcata
     2701 agtcgcaatc cccgccgcat gcagcgcgtc cataccgccc atcttgtcct gatgcgcgtg
     2761 agtcaccacc gccagcgcga ccggcaggtt gatctcctgc ttgatccagt tgaggatctg
     2821 ggcggtctgg tcatcggtcc aggcggtatc gaccaacagc acgcggccgc catccctgac
     2881 gatcaaaccg ttggaagcga ctgccccgaa acccggcatg tcgagatagg aagtgtgctg
     2941 ccagacattc ggtgcgagct ggcggaaaac cagatcgcca aaccgttggt cgccagtttc
     3001 catttgctgg ccaatcgtcg ggcggatttc accgggcatg cacccgctca gcatcaatgc
     3061 agcggctaat gcggtgctca gcttcgcgac cgggtgcata atattgggca attccatcaa
     3121 gttttccttt tattcagcat taaaaacccc gcaaatgcga ggcctagtaa atagatgatc
     3181 ttaatttggt tcactgtagc aaaaatatgg ggcgaattca aacatgaggt gcgacagttt
     3241 caaaagccat atgataatca acaagctgag caaatttctc taaggaaggt gcgaacaagt
     3301 ccctgatatg agatcatgtt tgtcatctgg agccatggaa cagggttcat catgagtcat
     3361 caacttacct tcgccgacag tgaattcagc agtaagcgcc gtcagaccag aaaagagatt
     3421 ttcttgtccc gcatggagca gattctgcca tggcaaaaca tggtggaagt catcgagccg
     3481 ttttacccca aggctggtaa tggccggcga ccttatccgc tggaaaccat gctacgcatt
     3541 cactgcatgc agcattggta caacctgagc gatggcgcga tggaagatgc tctgtacgaa
     3601 atcgcctcca tgcgtctgtt tgcccggtta tccctggata gcgccttgcc tgaccgcacc
     3661 accatcatga atttccgcca cctgctggag cagcatcaac tggcccgcca attgttcaag
     3721 accatcaatc gctggctggc cgaagcaggc gtcatgatga ctcaaggcac cttggtcgat
     3781 gccaccatca ttgaggcacc cagctcgacc aagaacaaag agcagcaacg cgatccggag
     3841 atgcatcaga ccaagaaagg caatcaatgg cactttggca tgaaggccca cattggtgtc
     3901 gatgccaaga gtggcctgac ccacagccta gtcaccaccg cggccaacga gcatgacctc
     3961 aatcagctgg gtaatctgct gcatggagag gagcaatttg tctcagccga tgccggctac
     4021 caaggggcgc cacagcgcga ggagctggcc gaggtggatg tggactggct gatcgccgag
     4081 cgccccggca aggtaagaac cttgaaacag catccacgca agaacaaaac ggccatcaac
     4141 atcgaataca tgaaagccag catccgggcc aaggtggagc acccatttcg catcatcaag
     4201 cgacagttcg gcttcgtgaa agccagatac aaggggttgc tgaaaaacga taaccaactg
     4261 gcgatgttat tcacgctggc caacctgttt cgggcggacc aaatgatacg tcagtgggag
     4321 agatctcact aaaaactggg gataacgcct taaatggcga agaaacggtc taaataggct
     4381 gattcaaggc atttacggga gaaaaaatcg gctcaaacat gaagaaatga aatgactgag
     4441 tcagccgaga agaatttccc cgcttattcg caccttccct aatggtgtaa gccaatctaa
     4501 cgcctttcta ggacgagtat tcagtgacat ggcaacttga tttaaataat gctgatctgc
     4561 ctgatttaaa tcaatccctt taggtaaata ttgcctaatt aaaccattca tattttcgca
     4621 tgtgcctttt tgccagggtg aatgtgggtc acagaaatat acatctatgc ctaaatcttc
     4681 ttcgagtatt ttatgttctg acatctcacg tccacggtca taggtcaacg ttttacgcag
     4741 ttctgcaggt aaatatttca gagcttcagt taaagccttg cgcactgatt ctgcctttgc
     4801 atcaggtaat gttgccaaga tacagagccg tgtatttcgt tcaataagtg ttgctatcga
     4861 acttttattg tctttacctt taattaaatc agcttcccaa tgacccggta tttttctttc
     4921 ttgaacttcg gctgggcgct catgaatagt tttaatatcc tgtaatatag aatctttttt
     4981 aggttcaccg ttagcttttc gctttttatt ttcatgacgt agacaggata ataagtcttt
     5041 tttcaactca ccctttggta atgctcgtat cgttgaataa atcgttgtat ggcttacatt
     5101 cattgtttga tccaaatcag gaaatgtctt taaacgcttt gctatttgct gaggagacca
     5161 taaacaacgg atcgcttcaa caataaattt ccagaggatt gaatcgattt tgagttttct
     5221 gtgaccacgt ctacgtctag cgaaggtgtt atcagaagca tatcgagctt gataaacgtc
     5281 attgatgcta tttcttttaa gctcacgata gatcgtacta ggatgtcttt taatgagttc
     5341 agcaaatttt ctggctgaaa agccttcttt tcttgactca agcattaatg cagtacgatc
     5401 ttcaaagtta agatgatggt atgacaattt tatatactcc ataaaccctt taaattaatt
     5461 aggtggttta tgtcgcactt caagttttac tctgccaact ttaaatcagt ggctccacta
     5521 acactcttca cttatttatt taaaatttag cgacgttaaa agacaattaa gtatttttag
     5581 ataatggggt tctagggatt ttccgtccaa aaacgacaaa gtgctctgga ggccgcgccg
     5641 tccgtggcct ccagaggggt attacttttc gctgacgggt aaatatccct cgattgagcg
     5701 cataagctcc tccagatctg gcaatttttc gctcaattcg aagtggtaag tccccttgag
     5761 gttaatgttg tgccaggcca caggggatgc ctgtttgacg atatctattc tcttggtatc
     5821 tccttggtat tcgaagctgg tcaacagctg gctgagtatc ctggagttga agtaaacgat
     5881 ggcattggtg accaggcgag cgcactcatt ccatagctgg atttcttcgt ctgaactgcc
     5941 ccggaactga tccccattga cgctgctcac ggcccgacgc agttggtgat aggcctctcc
     6001 ccggttcagc gcgcgctgaa catagtttct taaactggca tcatcgatgt agcacagtag
     6061 ataattcgct ttcaccaggc gattgtattc cgtcagggct tccagcagcg ggtgattgcg
     6121 cttgtactcc gagagctttc tcaccaaggt ggcttgcgtt gttttccgct gcttaagtga
     6181 tactgcaatc cgttggatgg tatcccagtg ctgcgcaata cgatgggtat tgattggctt
     6241 ttttaagcac agctgaattc ggtgttcttt gtcttccttg acatcaaaca tgtcattgat
     6301 cactttgcca acctgggcat agcgtggggc aaactggtat ccgaacagat ccagtaacgc
     6361 gaagttcaca tggttcaccc catgggtatc ggttgagagc acatccggaa tgatgtctga
     6421 cgtattgctc atcaacaaat caaagatgta gtgcgattca tgttcgttgg cgccgatcac
     6481 tctggcgttg atcgcagcgt gattggcgat caaggtcatg gcagaaacac ctttttgagt
     6541 gccaaagtac ttcgacgaat aacgggtttt gaaggtctcg cgccgggctt cgaacttttg
     6601 accatcggca ctggcgtgga tcacatcttc ctggatgttg tagtaccgga agatgggtag
     6661 cttggctgtc gcgttattga tgttgtcgtt agcagcattc aatgtttcca ggcgaagata
     6721 gttcgcctgg atagtgctga gctgatcata ggtacgatca gagatctgtg ccatgccgta
     6781 aatgccttga ttggttgcat tgccgaccag aattgccaac aggtcatatt catgggaacg
     6841 gcttctggat tgggaaccca gcacatgagc gaagcagtca atgaaaccgg tgtcacgatc
     6901 aaccatgcgc agtacatccg caatccccgt tgtgggaatt tgctggaaga agggattgtt
     6961 gaccagatga tgtttgctgg ccgaaggcag gcgccagaag cgtttaccct gcggattacg
     7021 caagatgata ttccggttgt cttcctgttc aagatattcg ccgacttcgt acaaacgggt
     7081 atccaattcc atggccatct gtttgatcag cttttcaggc tcctccgcta attttgtaaa
     7141 atggctctgt tgaagcagcg tatatttgtt ttttcgccaa tgttccccgt cgatcaggtc
     7201 ggcgtcgagt gcccggtatt tagtgatatc aggcagcgtc agctggccat tcaggcgatc
     7261 aggaatctgt tgatagagga accactcata acgatcgatc aggatattcc cttcaccatc
     7321 cagcaggaat tcacgtgatt ttttggaaag gagtctggtg tcggcagttt gcaactgagc
     7381 gtcctggccg ttgagttcgt tttgtgtttt cgccaaggcg gccgctaagt gctgggtgcc
     7441 gtcgcagcct tcgaagcgca gacataggaa caactctcgc agcaaacctt tccgcagact
     7501 ttctttctcg tcgcagtact gccacatggc ttcatcgacc gatcgtcgct gctcgttcag
     7561 gaagagacat acagattcca gatccctttt ggtcagcagg ctcagtgctt gctgtcttac
     7621 tgttgcaaac ggtagttgca gatcaatgct gtcatcaatg aacaggtgca gtacttcagc
     7681 tgccttgctg acatttttag ctgctttttg ccaatcctta aacaccgctt cctgcgcata
     7741 atcctttgcc ttctgtttgg tctgtctgac atgatgcaca aagccatcgg caatacgttc
     7801 cagcgcttgc tgccatcgcg tttgaagata gcacaataaa tacagccatt ggctgcctac
     7861 ggtttgacgt ttcagtttgg caccgtagta gtcgactttc tctgccaggt gttgcagatt
     7921 tttctgcgac agtgatagcg tgctcaacag cagatccact tccggcatcc aatgctggat
     7981 atggcgatag acaatcagtt ctttctccaa ctcggttccg gtgaagttac gagccgattg
     8041 tcgcaactgt cggaacggca gtggacctgt gccgttcacg agctccgcca gtgcttgctt
     8101 aagaccgcgt gacattgcac gctcaaggtg cgcggccatg tgttcctgtt cgtctcccac
     8161 cacctgactg atgatctttt gcagcgtgct gtaggcaggg attgcgatct tctgccccga
     8221 acaatattca ttggccgcat cgaagaggtg cctaggcgct gtccaggctc tggcttgctg
     8281 ggataagtaa tctctcaacg ccgctccatg gtctttgaca ttccagcgct ggtagttgca
     8341 gagctggaaa acacgttggt aaatccgttc gttctctttt ggcgtgagat taaagggtct
     8401 acagcctggg cctggtagaa cggtttgata aacgtatttg aggtcctgct tgatctgatg
     8461 aaagccggga ttcagcacca ccggcttggc tttgaaatag cccagcagta ccaccagcat
     8521 gtaacgctgg ccccgatgac ggaggctttt agcgatcgcc agttccttat cgttcagcga
     8581 gaaaaagaag cgttggtcgg cggaggtgaa agcggggggt ccatataact catcttgttc
     8641 tgcctcggac aagatttgga cacgttcttc gaatgccatg gatttagccc aaaaagacta
     8701 aatcttactc aaacagagcc caaataggga tctcgaaaaa agtaataccc ctctggaggc
     8761 cacagacggc ggggcctcca gagcactttg tcgtttttgg acggaaaatc cctagaaccc
     8821 ctcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa gtacaggttt
     8881 cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact caggcaatca
     8941 ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat gatgaagatt
     9001 accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc gttgccagag
     9061 ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt caggaagatc
     9121 aactgggcac actggggctg gtcactaacg ccgtcgtgtt atggaacact atttatatgc
     9181 aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa gatatcgcac
     9241 gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc ttcacgctgg
     9301 cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag gcagaaaacg
     9361 ttgcttaacg tgagttttcg ttccactgag cgtcagaccc ctgttaccga tgcaagaaca
     9421 ggggagtttc atgaaatgcc tgtttaggag tgattaaagg taacttgtgc gcagaaggtc
     9481 aggtgtgtgg tggttagtac agactagtga atgttgtaac taaactatat ttttattaaa
     9541 ggccggaggt ggtgttcctc cggccttaat tacgtttccg tgttcattag tcgatgaact
     9601 ggcggatcag ttccagaatg gcaatcagcg cgtttgcaag cgccgctacc gccctcagaa
     9661 cgatgattaa caggttaatc atccgttttc tccataaatg gagtaaagac accgtggctt
     9721 gctcttacac tgcctgtcac cacggcttct cttcgtcttg tcagttgtag agagtctcac
     9781 tcctgccaga gcacagataa ccagcaccac ctgtattacc tgtcaggact ttgaacctgt
     9841 tcatgccgca tttagcggaa tgcagggaga gactcaccac agagctaacc ctctgattat
     9901 acgtttatct aacgttcagg caaaccgtta acgcacagtc actgcccgtc atattgcata
     9961 atcaggcttc acggattatc atactattgt cagttgtaga gagtttccct tccgggctga
    10021 gttaccagct cgcaccggaa ccgtaaaaaa gccgacctca ccagtcggtt tttttacgtc
    10081 tgcggtttcc cgcttcgttt aaatctattt taaatgaata aattgttctg cggcaaaccc
    10141 aacaacataa ggacgttagg gttgatggtt gccgaaatcg cggtgcgtta cgtgaattct
    10201 gaggttttgc gagggggatt gggcgcgaga atttacgaga gagcgccgga acaatgccgg
    10261 gatcacaaac catcacccgt gaaagaaatc ttccggcatt gcccggtcag gtagtatgac
    10321 attcattgtc aacggccatc aatttataag ctgctttgca cttcataaaa gatcaccacc
    10381 agggctgcta aaacagtacc gatagccagc acgttaaaaa gtacgtcctt catctttacg
    10441 aacgacatac tgatttgcgt cctttccctt ttgtcagcct tctctatcat ccagcgttgg
    10501 tatgaatcac tgttactttt tctgtccatg ttccccccaa ttcatcaatg agcaccaggc
    10561 ggccaccagg cttcttgcca acaaagtaac ggcctcgcgg cagacgttcg ccagtcttca
    10621 gccgttcgat ccagtcctga aatgcagggc gcggtttata gctgtagcgg ccaaaatcag
    10681 ctaaagactg cgtgaccatg actgtttggc agccatgctt tctcatatca cgaaacgtat
    10741 tttccagtgc agccagtgta ttcatttgca cgttctctct gcttttccca tgaagatatt
    10801 aacgacactc aaaactaaca ccgttggcat gatcacggca gcatagccga cagcaacggc
    10861 ctgatatatg gtcaatgagt agtcgggctg tttatacaat acgactgaaa aaatgctccc
    10921 caaacaagca agcagagtta aaaacaagta atcgacaaat ctgtttttat agataaccca
    10981 tgcaacgatg cagggatatg acaccaggca caggataatc gcatttgtgg tatgcacgtt
    11041 tcgctccttg ttatttaagg gttgtcagcc agatcaaaca gattgttcag gctggaacgg
    11101 aattgtttta ccgatgatac cagctcctga ctccgcgctt tttcctgcgc cagttcacgc
    11161 cgtagctgcg ccatctcgtc tctgttacgc tgaagctcaa ggatagccgt cataaaggtt
    11221 ttgctggctg atgcctgtcc ggtcgcttcc tgcgcctttt taatggcctg atcttcgtcg
    11281 tcattcgttc tgattgtgta atgccccatg atacccctct tttgctgtca tttttgcgct
    11341 gccggcgcga acccgggaag aaactttgct ggcaaaaaac actggccaac agatgtgacc
    11401 gggaaaattt gctggcaatt aattttcgat gaaagccagc tcgcaatcgc cgcattcaat
    11461 attcagtccg gctttgcccc acacggcagc gccacacccg cgacactgat atttaacttt
    11521 atttcgtttt ggcttgtcct caaatgctgc aactgacaaa tcattgtcat tgctcccggc
    11581 catatcatct gaatggctcc cgttctgcgg tgacactgac ggcagcagag ccatagaaag
    11641 caccgcttct atatcaatcc cggcctcagc attttgatgc gccagagcaa gcgtttcgcg
    11701 ccattgctct attacggcgg tcatatcttt ttgttgttcg acctggacag ggaagcgatc
    11761 aaaccagctc aaagcaaatt gcccctggaa cagttccagt gtgacgcgct ggaagcggcc
    11821 accagggatc ggatagtcat taatcttctg gccgactttc gcaccaccag gtctcccggt
    11881 ggaggagggc ataagtccga tactttccat ttttgccgcc cactgcgcat tgtggtacgt
    11941 cttacgcgac gggttgccgt agtggtactg ccacatatgg cactgctcat gaaccagcgt
    12001 ttgcatgact tcaataagcg ggtacaccgg gaagtattca ggattgaggg cgatttcatc
    12061 aatcatcctg ccgctgccgt cagcagcaac aaagcgcctg taactgaaat agcccattgt
    12121 gttcttgccg cgctggaacg taatcagaca gtcagggagt tcgccgtcaa aaaggcgctg
    12181 gttatagaaa tcaaacgcct gttgtagctc gctgtaagcg cgttccgtgg ctcttgtcat
    12241 gctgaagttc gcttagcggc gttccgatat tcctgtaact ctgcaacaat agcagggaac
    12301 acatcagcgc aacgctgtgc cagcttaacc gcctttgaac aaacgcccat accatgtgct
    12361 cttttgagcg cctccaacaa atctaccagt tcctcgtcag tgattttttc agtcatctat
    12421 taagcttctc cattatctgt atcgtacgat acagataata taaaccacac ctttgatagc
    12481 ggcaatcttt tttgctggca atttcacgcc cagggcgggc atcgagcggg taatattgct
    12541 ggcaaaaatc aacggccaaa ccggctgcag gaaactggtt tgctggcaag cgtgatttac
    12601 tgctgggctt gacaccttgc cattgtatga cgcttgtttt agtgtcaaat gaacaaccag
    12661 cgcgagaaga atataggaag caaaagcaat ggatatcaaa cctaactcag tgatccacct
    12721 ggagccaggc gaaacattac ccggctatac caatctgaaa cccgtacctg ataagtattt
    12781 cgatgagtta aaggcactca taaataaact caatactcaa tccggtaata tttacctcaa
    12841 gttaaaacag atgtacgcct ttttggatcg ttttaacaaa gaatttgtga gcacgtttac
    12901 ctcctgtcaa aaggggtgct cttcgtgctg taaaatggat gttcatttaa ccgcgcttga
    12961 ggcaacacat attgcacaag caagtaaact tactgccaga gacaacccgc ttacgaccgg
    13021 gcatgagtca aaatgtccgt ttttgtctga gaagggaaca tgctcaatct acaattaccg
    13081 cccgctgtta tgcaggacat atcatgtact cactccgccg gagatgtgta atgacctgga
    13141 tgcacaagtc atgcagtatg gttctcaaag cgcgaacatg ggaaatcaca tctacaaaac
    13201 aatagcagag tggatctatt ttcagacgta tcactgcact ggtaagttag aaaccaaaga
    13261 tatcagagac tacttccctt atccccgcga agatatccaa agatttcttc accataaccc
    13321 cccgcgccct ttttgctaaa aaaaagcctc tgacgcagtg tcagaggctt gttagattac
    13381 tgttcgtcac cagcagtgct ggaagcatct tttttgatga gaaactcatc aagagaacgg
    13441 ccagctttta acgcttcgtc gattggtttt ggtgtccggc cacgaccaga ccagtatttc
    13501 gtttcgccgt tttcatcaaa ctgatattta ggcggcgctt ttggcaacgt ctttttgcgt
    13561 gttttttgcg catcttcgga caggccaagc agttcttccg gtgaaaaacc ttctccggca
    13621 atcaactgga gcagttcctg gcgctttctt tcgcgctctg cgcgttctgc tgcctcacgc
    13681 tctgcttctt cacgacgttc ttcgatcacg gcgttaagct tttcctgcat ttccagcagt
    13741 tgttcaaagc tggtttctcg cgcaaaaaca cgaacagagc gaatattcag gagtagcttt
    13801 tttgttgcgt caaacgtttc attttcactc atcgagttaa tccttatttt ttactgaact
    13861 gaaacccaag tttacccgta gttttatcct gcaaaacaat tgcagcgtca aactttttcc
    13921 cggttttatt actggtaaag cctttaatct ctccggtttt accttttttg attaatgtct
    13981 caacctgatt ttgtgtcagt ttttttcctg acacttcaga ccagatttta aactcgcacc
    14041 cggaacagaa aaagccttta ggccggataa taatttcttt actgcatgaa gggcaggggg
    14101 cttcgagccg ggacatggaa ccttctgatc tggattttgc ttccatatcc ggtttgccgt
    14161 ctttgtccgg gaatgttgtc ttgcattcag ggtagcagga gcatccccag aaaaagccat
    14221 tttgcccctt acgcttacgc aggaaaccgt tgttacaaac cgggcagggc gtggcatttg
    14281 aagaaatcga aattccgttc ctgtccagct cgtcgattaa cccctggacg tactcatcat
    14341 tttccttaat aaactcctca actgtcagct ctccggcttt gatctgagac tgcttttcag
    14401 cccacagagc ggagatatcc ggtttagtga tttcaggagg cagtgcagcg caaaactcct
    14461 ggccttgttt tgtggttttc cataccagct ccgaataccc cttttctttt tcgatggaga
    14521 taagcccggt atttgccgcc agtttctcaa gaatacccgc gcgggtggct tccgttccga
    14581 tactgccacg atcactgctg ccctcgtctt tcgcttccag cgcctttctg agagccggat
    14641 catcaatgaa ttttgccgca ctcgtcatgg cggccagtaa cgttgactct gtgaaatagc
    14701 gaggtggtgt agtctttttc ttgtccacgt ctgccgattc acacaggccg ctgtcattaa
    14761 atttcagcaa tgaaaggtca aaaccgtccg ttccggcatc ttcggtttcg tcctcgtcat
    14821 cactatcctt acccagcgct tcccagccct tttgtaccag aacgctctgt gtggccgtaa
    14881 aggtatcccc cttaatatca aagtggattt tcgtcttgtt gcggatcgca tccgggtaga
    14941 acagcccgat aaagtagacg gacaccaggt tataaacgtt ccgctctttt tcattaagct
    15001 gaataccggc tccgcttttt gtcgtcggga tgatggcgtg gtgcgcctca atcttactgg
    15061 cattaaaggc tttgtgtttc tggcctttat ccatatctac gatcgcagac gccagttcgg
    15121 gtacggtggc cgctatagca gtggcaatat ctccggcctg gtaataatgc tcatctgaaa
    15181 gatagcgatt gtcagaacgt ggataggtca gaagcttgtg agtttcatac agcccctgca
    15241 tcacatcgag cgtatctttt gcagaataac caaatcgctt tgcgcagagc tgctgaagcg
    15301 tggaaaggtt aagcggaagc ggaggcttgc tattttccgg ctttgtcgtc gcgatagtaa
    15361 caatagcatc cttacccgta acacgtccgg caatatgcgc ggcctgagct tcggagatca
    15421 ggcgtttttt ctcgtctatc tgatcgtcgt ctgttgtctg atatttcgcc ttaatctgat
    15481 gcccgttcat cgacagactg gcgaatacat cgtaataaaa gctttctgtg tgattctggt
    15541 tcgccagcgt gcgcatgttc accagaccaa gaacggcact ttgtacccgg ccaacgttca
    15601 gaacgccgtc atagcccttc tcacggcctt ttaatgtaca tccccgcgtc atgttatagc
    15661 caaaaccctg atcgcacagg gaacgcgcca gggcgcttaa cgtcataccg cgaaatttat
    15721 cgttaggttg taaattcgac agtgattttt gcactggcgc aaggttgagg tcggcaacaa
    15781 gcagtcgctg gacgggtttc ttgtttccgg cataacccag aatctcatca accagcaaac
    15841 atccttcatc atcggggtcg cctgaatgaa cgatgctgtc ggctttatcg ataagagaca
    15901 aaataatgcg ggtctgtgct tctgattcag gcttgatttt atattttggc ggataaaccg
    15961 ctttaatggg taaatcacta taacgccagt tggaatattt gtcgtcataa tcctgtgggt
    16021 cgtaaagctc cagcatatga ccataacacc aggtaacgac atcagaaccg tgttcaaaat
    16081 agccgttttt cttttctgta ttgggatttc cgcccagtcc ttcaaaaata gcctttgcta
    16141 aagacggttt ttctgcaata aaaagtctca ttaatcaatc ctcaaggtaa cttgaatagt
    16201 caggctgcgc accattcttt ttaaagattt cttccctttc ctctatgtct tcttctgcct
    16261 cctgcacact aaggtcaagg gttcctttca aaaaggcacg cgcattcttg attacatcta
    16321 ctgttaattc gttttttgtt ccgcgaatca tgccaatagc aatatttatt atctccttta
    16381 tttcagcagg agtcgttctg tccagcaccg cgccaagttc tttaaggtca atatttatat
    16441 cgtctggtaa gtacattttg gcacaagcgg cagcggcttt agtccgaaaa gcacctacag
    16501 atatgattct gtcaattctg ccagggcgtt ttctgatgcg cggatcaatc cggttaggat
    16561 agttcgttga aaaaataaca tatgtcccgg caagatttcg cggtgtatca gccccatcaa
    16621 gaaaacttaa cgttccggca tcagcccgat aaagttcatc aacctcttca gctattatga
    16681 taacgggtat tttcttttga gctgcggcat agcaaacatc tttaaagtaa tcatcagcat
    16741 aaacaaatac atatttatcc tgatattttg cccccaaagc ttttgcgata gtcgttttcc
    16801 ctgtgcccgg aggaccagta aaaagcactt ttcgcattcc tgactgacca taacgggtgt
    16861 agaaatcaat atccgaaaag aactgattaa agtcattctc cagctcatta aactttggat
    16921 gaacagaaaa acgtaaagcg ttttctattt cttccttcct tgccttaccg tgataataaa
    16981 cgccgtcgta acacccgatt ttccagatac ctttacgggg taaagagcgg tttaatttag
    17041 cgcgtccagc ttcgcaaaga ttatgaagca tcgacatact tttggttgtg ccaactgcgt
    17101 actccattct gactttagag ttaagaatga agcgaataat atgaaccact tcattttgac
    17161 caagatgaat ccggtatgct cccgaaccat ctttaaacat aagcttttta atcctcccgg
    17221 ctttagccat aacagggcta cttcccgtat gaacgacggt tgaagcaacg ggttcgatat
    17281 caagattcga ataaatttta cgattctttc tgtttataaa aatctctgcc actttaggat
    17341 agtaatcaca atcctttacg acagtaccaa aattatcttc accgtaatat tctttatgga
    17401 gccgtaaagc ctgctctacc agagttaatt tacccatatg cgcaccatgt atattaaaaa
    17461 ccgtgacgtt gcctgaagat tttcttcagg cagaggaggg gttggacaag cgaagcgcgt
    17521 cagttttgtt tatgacgcca ctcccacgga ggaacgggat tactgtcagc ccacaggccg
    17581 cgcttctgtg tctgagcttc ccgttgcaga gccggtaatg agttatcggt gttgtacctg
    17641 tcataaaccc atgcagcccc ttttagcact tgctggcggt tagcctcagt gccgttcgct
    17701 gtcaccactc gtcccaggac tcgcccgtag cgatctgttt gcgtataaga gacggtaact
    17761 gattgccctg ccagcagggc tttaagttga ttggttgacc agcgaccaaa gggttgtttt
    17821 ttttccggtg catcgatatt tatcaatcgg actcgaatcg gaacttcata cacaacaatt
    17881 ttcgcgggta gtgttttaac ttcgatggta tctccatcga gaacgcgaat aactttcccc
    17941 tgaatctgag cagcacacaa tgtagctggc aacagggcaa cagcaaagga aagaacatta
    18001 aaaaaatgtc tcattgcttt cttccctccc atattgctga gagtctttta aaaatggata
    18061 aaacgacgac taaagccact gcgataacag ccaacgaagg aagtacatag ctgtataact
    18121 caaccatgaa attaataaac tcaatgagcg attgcggctt taaggaagca tcccacaaaa
    18181 cgataacaaa acgataaagc ccatagacaa ttgccggaaa ccagaccata aacaacaaaa
    18241 tttcagtaaa aacagaagcg accgatttga cactatcgat aatttcaatt ccggcaattc
    18301 tcacacttct tgcatcaggt acgttaataa ttttcatcct cccacccctg taaaaaatgt
    18361 tcgcaagttc cagtctcaaa cctgaaactt gcgtattcac atcagaatta aatcaaacag
    18421 aattaaaagt ttttcaatcg gccaaagctt gaaataattt tacttactgt acctgcatca
    18481 gccgcaggac attcatttag ttttttcttt ctggcatctg ccgtgtgatc gggtaaaaaa
    18541 ccatgtttat tctttttcct gattgacata aaacttttat acatcgggtc acattctgcc
    18601 ggactacttc cgtcaagttt tccggcaagg cataacacca ttgcacatgg atcgttcggg
    18661 tcggcctttt cctcagcatg agcagttccg gcagcgaacg cacaaagcag cactgccgtg
    18721 gttacggtct ttttcatata cacctcatgg ataacgggca actaacgccc tgcggttgtt
    18781 caaccccggc tcaataaaat tacaagaatt ttattaatcc ggcgttatcg tgataaataa
    18841 acactggtag tccggcaaga aaaacagttc attttataac gatactgtat gacaccgttt
    18901 gcaccgttcg ccctcaacca gtttttagct gtgaaaaagt ctgttccgtt actggtttca
    18961 acaatcactg aatcagaatg tgcaacagaa taaaaaacag catcatcgta aaggcggttt
    19021 tcaggcctga aattatggat aaccttaacc cacgatgaat taaccttgtc tgatttaata
    19081 actgagtatg tcggttgcca gtccattaat tgtgtattca ccgtttcagg attctgttcc
    19141 cagtcaacct gtggtggttc aggcggagag gaacaggcag agagaaaaag gacactgacc
    19201 attaacattt ttttcatagt aaaacctctg actccagctc accgacggag agcatttttt
    19261 ctttagaagg atatttaagc cctttaacac ccaatacttt tttagtctta ttcagagagg
    19321 cggttaactc ggtaagtttg gggcttacca tcatcagcct gtccataaaa tacgggtcga
    19381 ggaagtagag ggctttctca gctttaaccg ggttttcacc tttaagaatg ataaattctt
    19441 ctttaaaatc cagcgttccc agctcctgcg ggagcacaag ggcgcgctgc gcctcacttt
    19501 ctgaactgct tttcgacgtt gagcgtccgc ttgtcttgct tgaacctgtc gatttggtgg
    19561 tgatatagcc cagcttttcc gaaatctttt tagcgtcatc ttcttcactg acagcgtaga
    19621 taatccggca gggatgcgca ctcatcagcg ttttggcacc ttcaacacca tagatttcat
    19681 taagctgact gatattctgg taaatcgtca gaagtttgag tttaaacccg gcaatataac
    19741 ccgaaccttt tttaatgatt ggcatgtaac caatagacgg aaactcatcc aggaacatca
    19801 gacagtcatg tttcagtgtc gggtcaaagt cagggttttc tcgtaacgtg acttccacaa
    19861 cgaagttaaa gaacaggttg aggaagtcat aggccaggga catatcttca gcattaacgc
    19921 caacataaac agtgatatct tcccggcgca actggcgaag gtcgaaatca ttgccgtctg
    19981 tacatttgcg aacagtcgga agataaaaca gactcatttt cttacgaaac gaaccatcaa
    20041 tactcgaacg ttgttcgtct tcggtttcgt gatactccct gattttggtc agggcatcgc
    20101 gcagatgata cagcgcgttt tcatcaagtc cttttgttcc ctccaggtct tctcgcttac
    20161 tgagtatcag ctcccggtcg atgttgctgt acaaatcgac tacagaacca attgagaaaa
    20221 caggtttaag cccgaactca ccaagccagg aggggtcgta gttaataaag aaatgaagca
    20281 attttgccag ccccgtccag tactgccccg cgaggttatt aaagtgtgct tccgctccgg
    20341 tcagaccgta agacgggaac aggatctcaa tcagcttcag caggtcttta gccccgctct
    20401 cttcttttaa atcaatatag aaaagcgggt taaactgatg cgttttactg ttgaaagggt
    20461 caagaagata gaccttatta cccagcagtt tttcacgtac cttactggtg attttccaca
    20521 gttcctgttt tggatctaat gcaatcaggg aatgtttcct gaccagcagg tttggaatac
    20581 ctattgcagc ccctttacct gcgcgggttc ctgcgccaag tgatacaaaa tctggtgcgg
    20641 tataccacag gtatttacct ttatatgcac ctacaagaat atcagtatcg ttttctttct
    20701 cccatttcag aagtttcgat tttcttaaat cattatcact ggcaaacttc gcgtcaccat
    20761 aaagagcaac atccgtttta ttcagttgcc agataataaa aacaggcaca atgagagacg
    20821 aaaccatacc tgataaaaga gaggggattg cggttaacct gatatctgtc cgtatattac
    20881 tctctgttat tatccgccac agcagcatgg aatcaaaatt cttccatata taaagcggcg
    20941 tttttccgtt caggaagtaa acagcaactg agccagtata atacgtcaca agacacataa
    21001 ccagaccgat aaaagcccac tggcctttat cgggtagttt cagagacata atcactcctt
    21061 cagcttttca aagtattcct gaaaatgaat atcacgatag taaatacccg atgcaaatcg
    21121 gttatcttcc tcatcgatgt acttaatact cataatcaca tcgacgttgc ttaacaccct
    21181 ccgcaatata atgttataag gcaaattctg acattcaggg ttcatatagc accgttgaac
    21241 cagtccaagc acagcatctt caggggtgct ctcatggacg gttgtaaggt tccctgcatg
    21301 gccggaactt gagcctttga gaaaatccca ggcttccgcg ccgcgaacct cagtcatgag
    21361 gattctgtcc gggttcatac gaaaaccaga acgtaaaaga gaagcggaac tgacagtgct
    21421 actttcccct tctgacgggt aatacaattt cacatgattt ttgtggaacc tgaactttgc
    21481 ttcatctgta tcttcaattg aaatacatcg taaatgatgg ggtatgtact gaagacaggc
    21541 atttgcaaag gtggttttac ccgcccccgt tccggcacaa aataccatcg ttttaccctt
    21601 aaccaggcat tcagggacaa attgttcaaa acaattatct ttaaacattg aagataattc
    21661 atcttcttta tccctgaatt tccccccttg atttagcctt gagtaaaacc cttgtttaac
    21721 aaaggtatca tggtcgataa acacactgga cggcttacgg attgtaatag acaccgtttc
    21781 ccgttccgtt gccgggggaa taactatttg tacacgctcc cctccgggga gcgtaacaga
    21841 acgtaaggga tattctggcg taaccgaccc accatcatga taatcagaaa ttgcccctgc
    21901 aaatgacata caatctttaa aatccatttt taattcatgc tgctgccatt tcccactcac
    21961 tttaacaaac agttcatcag gccgatttac cgcgatttca gttaaaccat cgacttcatt
    22021 aagccagtgg tggaagtatt catctattat ttcataaaca atatgtctgt tctctgcatc
    22081 cttcataatt caccttttcc tttaagatct tcaataaaac gcatgacttc ttcgtaatta
    22141 aatcgatgcg ttttgatttg tttcagtggt ttggggaaag gaacgccatt tattggtatg
    22201 cccttacgaa tatgatggta tatttcgctt tcccttattc ctaacagcat tgaaaactct
    22261 ttgatatcca gcgttatcaa attacctccg ggtacccacc atttttaatt tgtagatgct
    22321 ggagaaatcc aaatcctgac caacgatcag agtaatgatt tccccctggt ttttataaag
    22381 cgttggaggg atatttacgc tgtttgcaaa cgcttcgcgt gctatatcag caaaagcctg
    22441 gcgactgtta gctgtatagt cggtctgatt gtctttatta cttttagccg ctccacttgc
    22501 ggcctgtgcc acatcgggga tcatgccaac catcatcgcg cccataaagc gatctgcaaa
    22561 atgtgtgtca atccagcctg aagccccggc ctcacccagc gctcccgccg cctgtgtatc
    22621 aatcagcgga atatcaatga aaggttcctt acgtgttcta agcttcgtag cagcgataaa
    22681 cacggctccc tgaccatgat tgaacgtgcc cgttttatac atcagatatg cggctgttcc
    22741 cttttcaatt agctttacat tcctgtttgc actgtatacg tcctcattaa tcgtacagac
    22801 cagcttgcca gccagatcag aaataaatcg tctgtccagc gagcacttaa tcgcggtatt
    22861 ttcaggaatg aaaaggttag ggtcataagg aacgcgcgta attttagaaa gctgcgcggg
    22921 ttgccctgca ttaacaggct gaacctctgc ctgtttgtca ctgccgtccc cctcgttcct
    22981 ggtattgctg gccgttgatg agctaccacc ggaatttgag gaagcagagc tgctcccccc
    23041 ataggccaca tccagcgcac gactgaagtt ttgttgtact ttttccggtg gcgtttccgc
    23101 tttgccgctg ctttccttcc gttcttccgt cttagccggt tcttccctgg tgttgaatgg
    23161 atcaacatca tcgctgagtc ctgtcgcgcg ttttactgaa ccagtagata ccgcattact
    23221 ggcaggctta tcatcaccgc tggacattgc acgaataatc gacgggatat agtagacaga
    23281 caccccggcg gccagcagaa ggaaagcggc cacaataatc accagcccct tacgcgattt
    23341 tttaagcttc tcgataccca ggcgttttac tgatttaccg tcatcttcat ctcccttatc
    23401 cgttttatcg ttttgctcaa tttctttctg atgccgttcc cgtagtgcct gttcaatttc
    23461 tacagcggtc gggattttat tttcctgttc gctcactgga tcacctcttt ctcaacgttc
    23521 ggggaaacgg tatctcctgc cggaagacgg actttgccaa agccccggtt ttcaacccca
    23581 acaacctgat ttcccatacg caaaacaaag cgctggtcta caggaacacc aacaacggtg
    23641 aaactaccgc tgtttttgac taccgggtta gtcagcgttt cctgttcgcc gctcataaca
    23701 aagacgctgg ggattttctt cagggggctg aagccaaacc aggtatagcg gccatcgtca
    23761 tacgcataat ccgggctgat tgactggctg ccgtcagcta cccgcttcca gtaatcccag
    23821 ttcttgggcg acttcgcatt ttcaagagaa cggttgattg cctgctcctc ctgccgcttg
    23881 ttaagagcct caatccgttt cttctcgatt tcggcttgct ccttccggcg ttcctgcggg
    23941 tactggtaat taacaacaaa cgcgatctta tccggctgcc ggaacgttcg ggcgttcagc
    24001 tccatgctgt aattacgttt tgttgtgcga acaaacaggt ttgttcgcca gcgttccagg
    24061 tcgttttcag gatcaagggc tacactgacc acctctgaag actggttgcc attgtcatcg
    24121 gtactgttct tctgtacggt ctgtttgacg ggcttcactt cgagatacac caggttatct
    24181 tctttgttca cttcccagcc ctggggaaag ccggttctgg cactgatgac cgtttcatcc
    24241 tcgtcaaata ccagtgtggt tgtatagcca accgcactgt ttatgactgt cgtgttttgc
    24301 gggttataac tgacaatctg attacgggga tcaaaacggc tggctgctgg cgtaacggct
    24361 gcatggcaga cgtgcgacac caacagaagc gccacaatta gcgctccttt attcattttc
    24421 atagttttca ccctttcact tctttatcgc gtacatagct cgttacaacg aatttaagcg
    24481 ggttattgtt tcggtcaccc gatgccattt cgacctgtgg ttcaatacgg taggtcaacc
    24541 ggacgttcca gtattcataa tcatcctggc ctgtcgcgac atttcgaatg gttaatttaa
    24601 accggatata tgctcccata tccttatcat cgcgccggga tgacggactg ataatcacgg
    24661 acggattatc ctgaacagat gcggtttttt ccgctttatt ataaataacc tttggtgact
    24721 ggttgccgtt gattaaagcg ttgtaatccg tcgccacatt ctcagcgctg tataacagaa
    24781 cataatcata atcatgctgg agactgaaat agttataccc ttcacgacgc ttcacatact
    24841 gagtaatgaa gtatttcgcc agcgcttcat tttcagagag gttttctttt ttaacagaag
    24901 tcagtctttc agcaacgcca gtgacaccat taataagata agcttcgaca accgtactct
    24961 ttaacggtag cgctccattc agtgccagca gagacaacac acctacggca attcctgcaa
    25021 taccaccgaa tttataaatg cggttcattt tctcctgcat caatacattt ttctcttcaa
    25081 aagtagttga agagtctgtt acttcttttt tcttgttaaa accgggaagt tttaatcctg
    25141 agagtttaaa ccccattgcg cattacctct gctgaattaa caggttcgct ttttccggat
    25201 accggaggtt gtttatgtga cgcctggcat ccgacaaggc aaagcgtcac gatagccgtg
    25261 ataagtagtt tcataatgtg agaacctaaa tagaggagtg aatatttact gcggaatact
    25321 cagagttgac agatttaaat aataaatcgg ttaacctcca tatttctttt tcgccgcatc
    25381 tatcgcagcc tggcgaagcc tcttagcgcc aatattaacg ccctgcccgg tcagattccc
    25441 caacttaccg gatacaccag gagactggtt gaaccctcca tcctgacggc gaacgccttt
    25501 ccatgcgcca atccctgcat ttttacccat accaagcaga ttacgcccca tacgggaagc
    25561 gccgaacacg ccagcaccaa tccccattgc ggctgcccct tgcatcgcgc cttcaacacc
    25621 tacccctgcg agctgggaag cataggtttt ggcttgccag ataatccagg ccataaatgc
    25681 acctgcaacc ccggcctgag cacctgttga tatcagattt aattcatctg cgttagctat
    25741 cgagattgtc agaatgccat taaggaacgt cattcccgct ttaattgcca gtccacaaaa
    25801 caagaatatg aagcatgagc taaatattaa ctgaagccag ctattaaaca tctgccttaa
    25861 aaaaccaaat gaaagacaaa taataaacaa cggcgcggta atagtaagta tcttaatagt
    25921 aacttcggcg gcgagataaa ctattgaagt tagcaataat gcaattatcc ctccagcata
    25981 tgttaataaa gatgcgattg ccccatctgt tttcacatac ttcgaagtat ctttggacat
    26041 aagatatgca gctacctgct gaactttctc ccaaagctcg tcaacccatt tccaaggatc
    26101 acctcctgca aatgtttcct tcagcccatc aatggcctga gttgcactat ctaaccaccc
    26161 ccccatattt ttgacaaaaa caagaattaa ccagaatcga catagattcc ataaaaaatc
    26221 ctgcactgcg gttttttgct tcccaactat tgtggtataa gcataccaca atatataaat
    26281 ggaaatacca tacttaccca actgagacgc tattgaagaa aagttatcta actgaccagt
    26341 caaagactgg tcaagagttt taacaataac gtcatgagcg gcctggaaat acctagaagt
    26401 tgccatagtg gctcctttta acttccatct ttacccaata catttttgaa tgcatcttta
    26461 tagtcaacat gcttaggatg taattgctta tacctaaaca aaccttcctt agcgttttta
    26521 caattttcgc tatcactccc agactttttg cattcagcta cttttttcgt tgcatcgtct
    26581 acgtgagact gccaccagtc tacagattta acctcttcac acccggtaag aaagaatgta
    26641 agccccaacg ccgcaactaa tccatatttt ttcaaaatgc cccctcagtt catttcataa
    26701 ctctgctgct caaatgctgc cttataatca acaactggag tttctttatt aacagcccct
    26761 aattgtttct cctggaaagc agcctgtaac attttctcct tgtattctgc aagatcgcgc
    26821 tgcttatcgc gatacatttc atactgaaat ttgagcttgt taaatttcag gcttgccaga
    26881 gatacagcgt ttgtcgcgtc ctgcgtggct ttcgtgtcct gggcgttctt cacctggtcg
    26941 ataaggtcgg ccatttcctg attatcttca cgcagtttac tttccagctt attgccgtac
    27001 tcgatccccg ccagttggga caggaacgag gcttcacagc cccgcaccag gtcgcccgta
    27061 tatcccagcc ccttacaggt gtttgtcacc ttataatcgg ccagtagtga tctggcttgc
    27121 tcagacaggg tggcttccgg gttcttaatg taatcatcaa tgaaggagtt cccctggtcg
    27181 taaatattct ccagctcctt actgacagat tgcgcagact gcaccagtcc ctgaacatca
    27241 cgaatgcccg ttttggtcag cagctcctgc ttataggcat taatctgttt ctggtagtgg
    27301 ttcacggtgt cctgccactg tttgaggcgt tctgtccagc gctgcgcttc gacagcccat
    27361 tcagggtttg catcaatggc aacgggaaca ccagcctgag caccagaaat aaccgtaaca
    27421 gaggcgataa gtgccgcaac gatatgcttt ttcataggga ttccttaaat caggtttgca
    27481 cgttccagat aggtatcaag ccactcttcc ggcttcatac cctctttcca gatttcatcg
    27541 aacagttcaa gctggggttt cgatgcgctg agaaccggta ggtaatacgc cgcttttccc
    27601 aaatccagtc ttgcgaaagc cgcaaaatca tcactttccc ccttcctctg tgggtttttg
    27661 acgaccagga actgacgaga taacgggtca atggctttaa ttttgtcaaa ataaagttct
    27721 ctcacttcca actcgtctac gtactcacta cgtttcgctt tggggttcgc cagataaata
    27781 tgtgttgcgc actgctcacg aacggcagcc gcgataggtg atttaattag ttcatccggc
    27841 gattgtgtcg ctacgacaag caccatatcc agcttacgcg cggttttgag tttgttataa
    27901 acaaaatctc tgaacgcttc gttgttaatc cattgccaga actcatccat gtagataagg
    27961 agtcggcggc catcagccag catggtgaca cggtagatga gatagaatga agcggcgctg
    28021 gaaagtactt tgttatccag aaactccgtt ccgtctatac cgaaaacgtc caggttgtcc
    28081 acatcaaacg tatcgtccgg gttatcaaac acccagccaa attcccctcc ctgcacccag
    28141 gcattcaggc gtgctttaag cccatgccgc cgggtttcag tatcatcagg ctccagaatg
    28201 agtggtcgca acttactgat cggataagag cggtttgacc tctgcatcag gcgctcaacg
    28261 gcatcagaaa tcaggcggtt ctggtaatca tcaagcggct cactgttaag cgtacaaagt
    28321 agcctcacca ggtctttcat aaaggaaata tttcgtttgg ttggtggcaa agccgcaggg
    28381 ttccagccag tcggctctcc ttctttcacc cgaaagtaag cacctcccat tgaacgtata
    28441 ccgggttccg ctgcccgatc tttgtcgaaa aatactgtag ttaacttctt taatttcctc
    28501 tcaggtggaa aagactccgg cgttccgaac tgctgggcgg cgaaagcctt agtcatcatc
    28561 actaccgttt tacccacgtt tgacgtacca agaatttcgg tatgccccag cgtggggttt
    28621 ttaccgaagt aattttgatg ctcggtagtc atgtgatagt tcaggttata aatatcattt
    28681 cctgagccgc ccagggtgat aaggttgtta ccccaggtat ttcctttctc cttgcccgta
    28741 aagaaattat ggaagctttc catttcggca aagttcagac tgctgataga actcaggcgt
    28801 ggccgtaacg tgtagttacc aggtaactga gcaaaatacg ctgcgccaag actcaaggtt
    28861 gaataggtta cgatcagccc taaatcctcc agggtcgtcg ttacaatatt ggtgtccttc
    28921 accagacgct ccggcgaatc tgcgtaaaca atcagtgtct ggtgactttt cccgaaagaa
    28981 atatagccgc tggagaccat atcgagacca actttcaaat cagcgagcaa ggatttagcg
    29041 gcatcatcgg tcatttcgag tttgtcgatc tgatcatcca gcgccttgat tgcagactgc
    29101 ttgtctatcg gagttaaaga ggaagtctgt acatactcaa ccgggagata catcagtgca
    29161 tcaaaaattc cggcatccgt ttcctgaaag tagtccttga tctcgatgca acgaaagtac
    29221 cgggcatggt cagaggcagt gatttgtcca gcatcgttac caaagaacag gtctttacca
    29281 cccaggtatg tataaaacgg actattggtg actctgactt tctgccagcg cccggatagc
    29341 agtttctgga acagcgtaag ctgttctgaa taaactatat tattttcttc atataaccca
    29401 agacgacggg aatgaaaccg ggagagatag gtgctcaatc tgccacatat ttcattcata
    29461 tcattaatgg gttcttcgaa gatattttta ttaccctttt tccttgaaag aaaatgcgtc
    29521 actttatctt cagcgctgaa cggtttatag catacggtta agtataattt gttttcaaaa
    29581 tactcagccg ccgaaagcga ttcgtaataa tcgttcatca ctctgttgac gaatgggatc
    29641 ttgctgtcaa atacaggtcg cacttctttt ctaacccgaa tacgatgcgt ataaaaggta
    29701 acgggtttcc catcaaaact acgtatcagc gtattgagct ggtcagtaag caaggctaga
    29761 tccgcttcat ctacacattc aaaataagct ccgtcaatct gccaggtcgc cagtagatca
    29821 tggtttctgg taacaatgag gttatcagtg atgtgtgatg aatacgggat taactcttca
    29881 agagaagact ggtctttaag cttcataaaa ttgtccactt tcgatatatc gactgcgtcg
    29941 tacgaactcc ccgcataatg gacggcattg aagcgcttgt ttgccagagg attacctttg
    30001 atttgcattc gcagataaag caggtcaaaa aagcgttcat ctatcttcgt aactcgcttg
    30061 atggcatacc agccagggag gatgagggca tacatagcct catgaatcca gacaccaagc
    30121 aaaacgcaga taacgcacat tccaagaaag gggtagagcg gaacgcccag ccccctaatc
    30181 aaagcggggc gtgtcagccc tttaaaaacg gtactcattt ttacgccccc ttttattaca
    30241 tattacgcta accagtcagg cagatatggc gcaacaataa agatgataat cccgataatt
    30301 aaaggcgtaa tccacgctgc atgctggcgt gagaagatag cgatataacc aattaccagc
    30361 gcaatgatgg tgattgctgg cttacggata tccgtcagaa aatcgataat cttctgcaag
    30421 aagccagttg ttttagtgga tacatcatca gcaaaggcgg gttctgctac acaaagcatc
    30481 aatgcagcca taaatacaga aagagttaaa taacgtttat tgagttttaa catcgtttgt
    30541 ttccttttca tctaaaacag tgccacgcat ggcatattgg ggatatacgg taacttcttt
    30601 aggtttgata ctctgtgtta cattctcctt tctgattgca tctttaactg aaggaaccac
    30661 ccagtttttt ttgttatcag gcggattaaa ccctatgcgc tgtatataac tggtgttatt
    30721 aaaatctgat tcaggtttag ttcctgtttc tggattgccg gaataatagc aactcaggcc
    30781 acgtaaaata tcgccaccac gtttataaca atccaccaaa atttgctctg aaaccttgag
    30841 attttcgcag gggtcaaaca ttttctcagc ggttgtattg aagtttgcaa aattcgtact
    30901 cgttatttgc ataagaccga ctgagtaacg atgatttcgt gattcaattt gatttacgat
    30961 ctggagtgca gcctccttag ttttgggaaa gtaggacact acgcctttgt caccgggctt
    31021 acgttcaacc ttcggtatta tctcggcaat agcatatggg ttaaaaccag attcaaccct
    31081 tgcaacttca tgcgcagtat cgggatgaac gctggccgcg cattgcaaag caagcgccgc
    31141 aaaagcagta gtagataaca tgattttctc ccggccagaa cagcgcggaa agtttagact
    31201 gaatggttag tcatcgaagt cgtcatcgtc ttcatcgtct tcgtttatca gctcctcaat
    31261 agcctggctg gaatattttt ttatgaggta ctggacaaca ccagagcttg aaatacgtcg
    31321 ccccctgcga tagccaatct ccagagcaat tttatccagt tcctcacgag aagctgaggg
    31381 caggtagagt gtattacgtt ggggcttgcg tctcgttgcg gttttttttc gggtagcagt
    31441 catttttctt ccgtttcatt ttgtgggtta agcttctctc tgtgatgtag tggatgaaag
    31501 ccatacttct ttgctgcgga ttatccatga gtagaatttc agcaaaatcg tgtggaattg
    31561 atttccttaa aagatttgcc tgagctgttg tatgtgattg tctgtacaaa agctccgcca
    31621 tcacatcttc tggtacaggt aaaggctcac cgccaaatga gataaagcgt tgtacgtaag
    31681 ggatagcagt tattgtagtg gaatgaacct cttcaaaatc tgcctggata aaaagataac
    31741 cgggaaaaag cggcaaacgt ctttctcgat atgaaattat cttatccggg cgacgatacc
    31801 tttcagtctc cataggacag aagtaagaga agttcaggcg ttcgagatga gcaaaaactg
    31861 ctgcaaatct cgtagtggtg aattgcaaaa cataccattc catagttttt cctttcaggc
    31921 gctctctcga ctcggatgac cccgaatcag agaaaaaaaa caaaaggaca aatttacatc
    31981 tcataaaaac tttatcagct acatgtgctg cgttcaacca cacaagcaga tctttagatc
    32041 gcatttacga cacatttcat tttgtttgtg cttgtatttt atttatatct agatacatat
    32101 gctatttgat aacaaaaaag cagatcgatt gatcaccatg cgcttatttt atagtcaagt
    32161 taactaaatt ttttaccgct agttactttt tacaaaaact ctgctaacaa gataaataaa
    32221 tcgatcttaa tcaataagtt accggagagt atgaattatc ctaaacgaat tccgattttc
    32281 actttctgtt cgctttgaat catcgactgt tttatttgcg agaaatttct catctggagt
    32341 tgctgtttct taatcatctc cgcgctgttc agcaccagct ctttactgga tgaagagatt
    32401 ctgtctatgc ccttaacccc caggttctct agctgccact cattcctgtg tgcagtcttc
    32461 cagccatgct gtacaccgtt cttatcaagt gcagggattt tcacctcctt ttgtcctaac
    32521 ttcttcagct taacaagatc acctttctta acgttctcgc gagtggttaa ctcaccaaaa
    32581 tcagcccccc aatatgtttt ctccacgccc ttgttcagtg tcttaagctt tatgaaatgc
    32641 tgctttggtt tggttttatc gttctgataa tggtcgtagc caacatcaac aacctcataa
    32701 acgcccttct gtcgttttgg cgctgttttg tgcgcatctt taatagactg gttaagtcca
    32761 tgctgctgct tatgagtggc tttaacgtca tagcccttca tcttcagctc ttcacaaaaa
    32821 cctgtacgaa tttcccgtaa atcttttttc ctgatatcag ctcgtttacc gtcattatca
    32881 cgaatacgaa aaacgacatg cacgtgtgga tgttcttttt tgtcgttatg atagccaagc
    32941 acaaaacgat gatttgggta cttcttgttc atcgttttcc tgacagactc aagcagatct
    33001 tcaggtttta cctttgctga tacaggcggt gaaaacacaa tattttgggt tactttttta
    33061 ttttccttac ctttatcatc gaaaacattt tgagggtcat tagcacgatc tatcatgtgc
    33121 tctttagcct cctgaatctc atcacctttc catacctgac cgctttcact catcactggc
    33181 agctctgact cccggctcat atagtcaata ctgtttcgaa ctccctgccg ggtaatcgca
    33241 cctcctgaga ttttgacaac aacctcctta ctggcaccct tgcggttgat acgttcctga
    33301 acgttgcgtt gatagttttt accaccattt ttaactttat gggcgaaagc agacttgcgg
    33361 ccagcttcag aggacttacg cttaaccctg aattccttgt caacataaac gcccatgatt
    33421 acacctcaac cttgttagaa acagttcgcc ctttgcataa cataatgtag ttttcaaact
    33481 cacttttgag actcattatc tctgaactca accgctcagc gtcctgatag aacccatctt
    33541 tatcaagtac tctggcatta tcattaacga taatataatg aaagtttcgc cctattttat
    33601 ctattgcatt acggcaacga ttcatcacca gaagttcctg atcgaaaaaa tcaagttcat
    33661 tttcaagtgt cgtctggatt cgataacgac attctctgga taaattccag ccatgacgat
    33721 gagccgcttt ctctattaaa gttctttgtt tccctgtcaa atacattgtg gttttgataa
    33781 aatcatcatt gtcttttgac acattaacat cttcattttt aaccttgctt ttcagtaaag
    33841 cattggcaag cagcttcatt ccttttgcca cggttaaacc cggttcatct ggataaagtg
    33901 ttttaagaca atcaagtaga tcatcatgtt ggctttcttc aatcctgaat tgaacttttt
    33961 tcataaaact ctccaacaag aaccgactgt aggtcaccgg gcaaacgttg cggaatggcg
    34021 tcagagacgt cattttgcgg cgtttgccct atcctgcatc gcagtgaact acctccacac
    34081 ataaattgtg cttcagcgtc catcgaaatg caaaaaatga aaggctatcg tggctgtgat
    34141 ctggtgcgtc tgcgaccgga gacaacggat tcgcggctaa cttgggcgga aataaattcg
    34201 ctacgcgaat ggcctggcag ggggcgcgag cgctgtttta cggaatatac aaaaaaagca
    34261 cctcccgtaa acgggagggc ttcggcgatt caggaacggg aattttattc tgcctctggt
    34321 gtgtcgcctt ccatgccctg ccgggcatca tggagccagc ggcggaggtt actcattctt
    34381 tcccggtttg cccggctggg actttttccc ctgttaccgc ttagtatact ccagactctt
    34441 aacccggcct cgttagtcca gtgcaataca atttcccctt tgctgttaaa tcccagcgaa
    34501 aaccggacag ctccccgcct ctgttctgca ctgataaaat ctaaaaactc ctgttttttc
    34561 atagcacctc ctgtttttct atcagtgctt ttgtttcttt cttttccagg gcttttctga
    34621 gtaatgccat gtactcagga aaagcagaga atggcatcaa gggaaaaacc ggacgcccac
    34681 ccttttcaac agtctgtatt gctgataaca tgcgttccga cattttatat tcctcgcgag
    34741 ggaattgagt gtcacggtat caggttgcct ccacaacctg ataccacccc taattgatta
    34801 actccgcagt tctcttatcg cttcattttt ggcttcctcc agttctttaa gggtatatgc
    34861 gaaattcttc cagttaatta cttcgtttat aacctcagat ttacttaaac cacactcatt
    34921 aaaccaaaag ttaatatttt tcttgaggga atttttcgcc gtcagttcct cggacaacaa
    34981 ttcactaaca gacgtgataa cataaccacg accgcgattt ttatcttcga aagaatgaac
    35041 ggcaaggaag agagtttctg aatggacaaa agcagagcag gtgatttttt ctttcttagt
    35101 gtttaaagcc tggaaagtga taacatacgt gttcatttta gtctcccgcc cgtttgggct
    35161 actggatcat ggtaaggaac tattcccgac ctacaaataa attataccat catatgatgg
    35221 taatgcaatt tatttagtca ctttatcctg atttttttct ctcttaacga gctggtaatc
    35281 tggatgttca tcagccagga gcaaaagtaa ttcatacgta ccagcggcta aaccacgaga
    35341 cgttttaccg gatgtttctt tggcttgcca ggctgataac gtaaaaccaa accgttcagc
    35401 ggcttcctgc tgcgtaaagc cagctttaat acgtgcttgt ttcacctctt caggtgttgg
    35461 cttaaaactc atgattttgt accttcacaa tagtatctgt atagaatttc aataaaattg
    35521 gatcatcaat ttcatcttta acgttcgaga attcttcgta ataaaatgat ttttggaaaa
    35581 caatcttttt agttgttttt ttatccgctt ctttagagcg taccttcatc agtttttcaa
    35641 gttcaataaa caaacgtgtt gcatcttcag acacaacaat cctgtatgtt acattacctt
    35701 tgtttttcct gtcgctcagg acttcattca ttttttgcct catagctgtt taataaaccc
    35761 cgctaccttt atgagctgat ctacactcag gccacgaatt accatttcat atccgtcgcc
    35821 ccgttctgat acgttaccat ctaacccatt ctcaacttta aacccggtca atgtcccatg
    35881 ccagctttca agatgcacta aacgagatag cattcctttc aggatcagtt ttttctcgcg
    35941 tttttgctgt aactgttgtt catactctcg cgccatatct acattatcta aagcatccga
    36001 caggattttc ttttcaatat cggcggcgat ttctacaggg ttacgctctg gcgatactgt
    36061 gcaagtatgg taaggacttc tccagctgcg actatctacg cttccaatga taactaacct
    36121 tcctttctcc attcgaatat gtatagagta atttttaaaa tcaggtgagg ttagcttaat
    36181 cttgtactgg caatcatcga gcagatttac acgccagccg ttccccagga ttcgacaaac
    36241 gatttcaaac accggtttat accgttcaaa gaaatttttc tgttctttca tcgattcagc
    36301 cttttgaact caataaaaaa gccgtaaatc gaattacggc agttttaaga ctattcatca
    36361 aagtgtccat catcacttgc agcttcctgg gcttcgcatt cctgctgata tgcttcttcc
    36421 ttcgcaaaaa gatgcgccca tccttctgcg tttaagccac ggtgcaagac tgaaccattt
    36481 tcattgcatt ctttggctgt aacttcgatc tcatccgggc aaatgtaggc tccgtaactt
    36541 tcaaaaatgt ttatcagctc cgcttccgtt atggtcttgt ttttcatcaa cgtgtctccg
    36601 aatcaatctt tgccatgttc tagggggctt tcgcccccat tccggttatg cgcttgcttc
    36661 tacacgtgtc cagcctgacg ccttacgttt acccgctgta ccatacattt cacgaatttc
    36721 ttcaatgctg tgttctttac ggttccagcg gcttttgtgt tcttcaggac ggtaaaacca
    36781 ttgttttttc ttagatgccc acttacagcc catttccttt aaaatgtctt tgtgttcttt
    36841 agtttctccg ctaatccaga cccaattacc aataacttca tagacaatcc ctgtaaggcc
    36901 ggaaagcgta ttcagaactt tttccagatc ttcaccgtag ttgtaacgtg cgttttcatc
    36961 agtgctttga aactgattga ttttatcaat gttagccatg agaaaatcaa acgcagcatt
    37021 tactgctttc atcagctcgg cacccaatgg attgcggtct ggatgatatt ttaaagccgc
    37081 ttttttgtat gccgccttga tgtccttttc agtcagatca ccggataaac caaaaacgtt
    37141 taatgcttcc tgaatattca tttttcgctc ccgcccgtgt gggcttctgg aatctggcag
    37201 ggaaccattc ccaaccaaca aattcattat accatcaaat gatggttatg gaatcatttt
    37261 taatgcttta cattgtaaag attacataaa gatgtatatt gctttaccgc gcatgaaggg
    37321 atttccgaaa gaggcgcgaa ctgtagttac ataatgcgct gtcgtaacat cgcgccggag
    37381 acgttcgcca ggactggcga accaacaagc ccgaatgaga gcgataaccc ccctcacccc
    37441 tgccaggcca atttctgact atcagacgat gtttagcctg tcgcagttag cgcgtgagct
    37501 tgcgaacggt acgccgggtc agtgtgaaac actggcgcgg ggcgctgtat ctgcgcgtta
    37561 tgagatgcgt tagcagcgaa ttacggggcg ggttgctgaa accgattgat gcctgacagc
    37621 aaaaaatgaa tccgggcgcc agctgctgca cgaaaattgc cagcaaaaaa cacttggcca
    37681 tcctcgccgg tcgtgaaatt ttgctggcgt aagaaataat ccccccggta tgaggggctt
    37741 gtctgaaagg ttagtggttg ggcttactga gagtgaactg taacccggct actgctgcaa
    37801 gctgtgcaag tgttgtgatg gtcgggtttc cgttcgggga taatgcgcgg tagatactct
    37861 gacgagcaag gccggatttt ttggccgcct ctgttatacc ccctgattag ggatcaggtt
    37921 aaaaacgagc tgtatgacgt tctaagcgca ttttacccgc tccgttgaac catgaccggg
    37981 ttaaagcggt taaaacgcct ctgagggctt ttgagagcat ttaagaattg gatcggaaaa
    38041 ggatcccgtc tggatctttt agagagatta caccgctcgc cgcagccgaa cgacagggcg
    38101 aagcccgaga gtgagcgagg aagcggaatt tccacggggt ttcaatagag cacttacgcg
    38161 ctaaggttaa tatttgctca atttttggtg gtgcagtgct cagggaggcc agacggatct
    38221 atcctctgga tttatgctga gttatccaca gacgctgtgc attaaaagcc ttatattttc
    38281 ttcttgatct taaaaactta aaaacttagc tcgcttcaat tacctgaaaa ttatggaaaa
    38341 aaattgctaa aggtgagagg atttttcacg taaggtgaga ggatttttca cgtaaggtga
    38401 gaagattttt aagttaaggt agttagattt ttcactaaag atgagaggat ttttcacgta
    38461 aggtgagaag atttttaacg gtttccacag tttgactgaa catcaagtga aacatgcggt
    38521 tgttgctatc tttagatatg aagatcctca gatcttcata tctaaaggtg agaggttttt
    38581 taattaaagg ttgtattgtt gtcttgaatt acaacctttg tggggttatg atttgcctac
    38641 ataggaaagg ttatatgagg cttatcgtga agacagtaac gggattaacg aaagttagac
    38701 atagaaatga agttggggta actcttgcat ccctttccct ttcagcaaaa agagtgcttt
    38761 ttctggctct ttgccagatt gatacaaagg aaatgttaga tgatgatatt ttggaggttg
    38821 atgctgactt tttttcaaaa gctacttctt tagataaata tgcctcttat gcagctctga
    38881 aagagggagc taaagttctt tcttcaacta cgttagtttt aaacagagat gatttaaaaa
    38941 atttggctga tgaacttggg atactatcat ctaagaataa aatacctgat cgcttggatt
    39001 taaacttaac agaattctgt gcctattacg atcatcttgc aactgtacgg ataaaattca
    39061 caaatacagc taagcgatat ttttctaagt tgattggttc tgaaaataga tataccactc
    39121 aggtattgaa atcagttgtg ttgttgaaca gtgtaaactc aacaaacctt tatcaagtca
    39181 ttaggaaata ttacagtcag aatagttcaa aaaaatcttt tgatatatcc gtggatgatt
    39241 taaaagagga aatgggactt tatactattg aggaagggga gaagaaatat aagtatccta
    39301 aatactcatt ttttgttcgt gatgttatca ataaaagcat taatgagata attgagaaaa
    39361 cggaaataaa tcagttatct ttctctgttg ttgggaaaaa agggagaatg gctcacatgc
    39421 ttagatttga gtttagtata aatgaaaaat cctcatcctt ttcggaagat gatatggcat
    39481 ttttagagga gtttgacaaa gtcgttccac caaaaaagaa taagtgaggc aaaacatggc
    39541 taaaatatat gagtttccgc agggagccga acgaagtaag ctgaaaaaag aaatcatccg
    39601 ggaacgtaaa aaacgattgc gagaaacgaa cggtaacccg gttatccggc acgctaaatg
    39661 gttctggttt tatctacgtc ttgctacggc tggcgcactg catttagtct ctgttatttc
    39721 tctggcagtc ctgggggctt tcagtaaagc tattttctgg attggtggaa tgctctgtgt
    39781 tgttacatgg ttccaccttg agcgccagtt ctggacgccg caaaatttca cgattccagt
    39841 aatcgtaacg ctgtggggat tgagtttgtt tgccactccc ctgatggagc tgcttaacaa
    39901 aaaaatgccg tggtatcgtc ttctggttcc tgatgcaaag cacactagca ctgaggcaac
    39961 caatgatgat cagccgtgaa gcaacaatgt tatccagtcg gcttgcagtg cttctttcag
    40021 ccttttatca tcggttaatc atagcgcacg aggcgagttc ttttcactgc gatgcaggat
    40081 agggcaaacg ccgcaaaatg acgtctctga cgccattccg caacgtttgc ccggtgacct
    40141 acagtcggtt cttgttggag agagttatga aatatcaggt taaagagttt attaacgaaa
    40201 aatattctaa ggctgttaat attttaaagg ataaccttaa agaacactac catatttttt
    40261 atggtttgag attaagtgag attctttttc ctgccagcga atacggctct gaaatgtttt
    40321 ttcaggagtt tgaagcgatt aacagtgtaa tattgccttt ggtgattttt gatttgatag
    40381 accgaaagcc gattatggtt atcggcttcg gtgaagtatg tggagttgat tcacttgttg
    40441 attctggtat agaagttgtt tctttagacg gtttaagcga tctgctactg gttgaaaaac
    40501 tgacgccatt atttaactaa acctctggat aactatcttt tcagttttcc agtaagattt
    40561 tttctatatc tgaaagcact attttttcaa tgctttctat atagtaatgc tcattaaaac
    40621 ctgtcatttc tgtaaatttt agctctgcat catctttcga ataagcaata acaactctga
    40681 aatcattttc ccacaaatcc cacctgtatt tgtcttttat acgaaccctg tacaatcctt
    40741 tttggatagt gccatctttt ttctcttcct gtactttttt taatttttca ttttgcacct
    40801 gttgttcaag ccatgattta ttcataaatc ccccacggtt aacgtcttta ggctcggttt
    40861 ttacttaacg aaatcttccg gttttacacc tgtttttacc ttcatatact tgccctcccc
    40921 ccttagcttc ggctgaagtt cggtaaagta ccatccggca acggtaataa tgcctaaaaa
    40981 tacaaaaata gcaggcttca gaacatcaat agctgataca gcgcataact caatattgag
    41041 attagtacaa gttccctcat caggtgcata gaacccccat aagcctatac ctataaggaa
    41101 catccccaat atcggttgga tgtataacaa gtagatatta ataagagttt taatatatct
    41161 gaatatgaac atttttcttt ccttaggttt ctttctattt tggatttcca attcttaacc
    41221 aatgtgatgg ttcgacaatc cattctccgc agtcataggc aacggcctta ttatgcctgt
    41281 tagcgaacca tactaaccag gctaataatc gctgattgcc gctgtatttg gtttatctct
    41341 aaccatcaca acatatctct ttttcttagc cataacgcta ccctaatttt gcagattcaa
    41401 gcagccagtg gtttttcagg ttctcgtcca ggttatcgaa tgcggccagc gctgccttta
    41461 gaacggttgt cttgctctgg cctgttctct gcgcctccgc ttccaggatc tcctcaaagt
    41521 acgctggcag tctgaagttc atcaactttg gtttacccgc tacaggcgct ctggtagcgc
    41581 cggagataaa acgcgcggtt tctgcgtttg ttgctggttg ctgagattca tcgatgttcg
    41641 ggcggtttag ttttaaagcc atgaaaattt ccccttgtac ggtatgcatt aattgtaaat
    41701 atcatagtat ctttatggtg tatttacaat gtaaagattt cgatgaatat tgttgatatt
    41761 tctataaaat gccaatttcc tgtgccacaa gttcaatctg tgctttcgct gttgaaaggc
    41821 tcgatgcccg gctaacgtca tgtacccctg ccccttcatt acaggcgctt tcatacacat
    41881 ccagctcgga tatccgggtt ttcagaggtt gaatccagat gttgtccgaa cgcagtaatt
    41941 tatccagatc aatggccgct tttctgtggc ggggtttcgc tgagttgatt ctggtgaaca
    42001 gcacccacgg ttccagagct gcgttagctt tctgagcggt gcggattttc tctgtaacac
    42061 tggtcagtgt ttcgctttcg aaatcagagg acggcttaac cagtgtcacc acgatatctg
    42121 atactgtcag ggcgctacgg aactcctggc tgtcgtggcc ggggcaatcg acaatgagaa
    42181 cttcacacag cttactaagg cgcttaattt cgttgctgac gtttccgtaa gctgcgtgaa
    42241 ccgggatagt gagtaagccg ttatctgtcc gcttttcgtt ccagctcaac agatcgtggt
    42301 ttttatctgt cttcaggata attaccgact tacctttgtt gaccagcatt gagcctgtgt
    42361 tagccacgta ggtgcttttg cctaccccac ctttatctga tacgacaagc aagattttac
    42421 ccatttcaga atccctctct ttatgttgta aagctcatgt aaagatatga atgtctttac
    42481 atggtgtgtt tttcgtaaag atacttttgt ctttacagtg tctttataat gcaatggctt
    42541 taccatgtaa agcacaaata aagatatatt tatctttatg gtgtctttac atggtaaatg
    42601 ttgctgtgtc tttatgtgcg ctttatggtg taaatcttgg tgtgtcttta catcatcttt
    42661 atgttgtaaa tttttagtgt gacgttgtca ctatagggct ggggtctgtt tgctggttct
    42721 ttttgagtgt gccttccctg aaccaactga aatgggtact gtatgggtgt gtctggttaa
    42781 ctcaaaatga actggttccc tataatgact caaacagtac cgaaaggctt tgtcagagct
    42841 tacttacgcg catccacgac tgaacaggat gcaacacgcg ccctggacac gataaatacc
    42901 ttcgccatcg agcgcggcct gagcatctgt aactactaca ttgaaaatga atcaggttct
    42961 aagcttgaac gccctgagtt gttccggctc ctgaaagatt gtcagcaaaa cgatatcttg
    43021 ctggtagagg atgtggacag gctttcacgc ctggtagggg aggactggaa cacactgaag
    43081 aaaatgatcc ggcagaaaga tatccgggtt gtggccgtca acgttccgac aacctggctc
    43141 gcatcgggtc acaatgattt tgacagccga atgttttcgg ccataaacga tatgttgctg
    43201 gatatgctgg ccgccgtcgc caggcgagac tatgaacagc gccgggaacg gcagcagcag
    43261 gggatcgcca gggcgaaaag ggaagggaag tataaaggcc gccaggttaa tcagtctcgc
    43321 tatgacgcca taaacaggct gattgcgagc ggcagctcct ggagccagat tcaaaaagtg
    43381 ctgggttgta gccgggcgac tatcagcaaa gctgtgaagc aacagaccgt taacaaaatg
    43441 aaccatgaac ctgaaggtag ggagtagtta caacataata aacagtgtgt tgaagaataa
    43501 aactgattct ttatactgta tatgcatggg tcttccccga tcatggtggg aaggctcaga
    43561 acgccatatt cagctttccg tagtggaaca tcacccccag tttaaagcgc tcccggtttc
    43621 ggtatcccct ggcttttatc ctcagcagcc tgatcttgct gttaagtgcc tccgcatttc
    43681 cgtttgagac actgtgtcgc atcgcattca ggatcccgta cagccttttt cctatcgttt
    43741 tcgcggcatt tttcatcatg ggaacgtcac tgttagccgc cagcgccaac catctctgcc
    43801 agtcactccg tctttcctcg ctccatggcc tgttccagat atcctttgcc agctctttca
    43861 gcgcccagca ctggctcgtc agcttcatct gtgcacgcag ccacatcagc ttttcctgcc
    43921 gggattcggt catccactta tcgctgtact gccacaggaa gcgggttcct tttgcctggt
    43981 gtcggctttc aacagggagg tgcggatgtt cattctgacg ggttttatca actacctcgc
    44041 ccagttgctt cgccacatgg aagcggtcaa aggcgatttt ctcaaccgca ctgggtaagt
    44101 ggatacgcgc tgctcttata tagcccgcgt tcatgtccat tgagagcgtt ttgatagcca
    44161 gcaactgccc atcagtgagc gtgcgaagat agccggcaag actctctgtg ccgcgatcat
    44221 ccgttaaggc cagcgcccga ccatcgcgat cggagatcac cgttatgtaa cgatgtcctt
    44281 ttttaaaggc gacctcatcc acattcatat gacgggcgga taatggcttt tttatccggg
    44341 caagacctcg cttaactgcc cgggtcataa tgccgtcaac cgcattccaa ctgagcttaa
    44401 gttgcttcct gacagcatca acggtgctga ttttcagcca tgagagaacg aacgattcga
    44461 atagcaacgt ataccggctt ccggggccag cccacggaac aggcaacgtc aggcagccat
    44521 gctccggaca cataattcgt ggaacatcgg cttcaacaat agtggtgaac tggcaggtat
    44581 caagatggcg ccatttacga tgacggtgat cgtgaacaga acaggattta ccgcaggtcg
    44641 gacaggctag ccgggtgttt tcagcgatct caatagtgac agtaacagaa ccggcatttt
    44701 catcgagaga aagggacttt acctgccacg gatcggacag gttgagaata tgagcgtaga
    44761 gggacttttc gtccatggcg gtgacctctg gcgattaaat acaccattat catgccttca
    44821 gccaccacaa caagggaaga cccatatgca tacagctata ttgatcggag gtttttcatg
    44881 cctagacact atgaaattga ctcagcctgg cgtgcgagca ttaagcgtga gccgaacggt
    44941 cgtcagacag ttacgacaga agcctttgtc agccagcttg cgctgattaa ttttcactgg
    45001 agttgtcgcc aagcaaatca gtggattgaa acctacgtga ccgtttttaa agacatttcc
    45061 actcaggaag gagagaaccg gacattcatg ctttttaacc ccaacggagg ccgttaaaaa
    45121 tgggttttcc atcgccagca accgactacc aggaaaacag gctgtcactc gatcagcact
    45181 gtaatacagg agcgccggga gtattctttt ttcgaagtga tacctactct ttccgggaag
    45241 ggataaagcc tggtgcgctg ctcatagtag attttggggg cacgcccgtt gacgggtctt
    45301 tggtgctctg tgtcctggaa caagaattca ggatcatgcg attgcgacta caccccaagc
    45361 ggtgtctcca ggaactcgat aagctggata acttcagggc tatacctgat gacgatgagg
    45421 acgggttgga ggtcaggggg gtcattacgc acatt
//
LOCUS       DACAPB010000007        44981 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.007, whole genome shotgun sequence.
ACCESSION   DACAPB010000007 DACAPB010000000
VERSION     DACAPB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.007"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..692
                     /locus_tag="IT904_000377"
     CDS             <1..692
                     /locus_tag="IT904_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP3190212.1"
                     /translation="EGAEHPHKENTGGNQHHNSDNETGETADHSIKVNGHHEITSTSR
                     TCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRNTIK
                     WWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSY
                     ERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSVI
                     WQKLIPSQADS"
     gene            780..1016
                     /locus_tag="IT904_000378"
     CDS             780..1016
                     /locus_tag="IT904_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HAP3190213.1"
                     /translation="MIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREYKHVAH
                     DGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1051..2331
                     /locus_tag="IT904_000379"
     CDS             1051..2331
                     /locus_tag="IT904_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP3190214.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLPDNPLKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(2351..2461)
                     /locus_tag="IT904_000380"
     CDS             complement(2351..2461)
                     /locus_tag="IT904_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP3190215.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(2519..3538)
                     /locus_tag="IT904_000381"
     CDS             complement(2519..3538)
                     /locus_tag="IT904_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HAP3190216.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(3550..4764)
                     /gene="rspA"
                     /locus_tag="IT904_000382"
     CDS             complement(3550..4764)
                     /gene="rspA"
                     /locus_tag="IT904_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAP3190217.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4970..5296)
                     /locus_tag="IT904_000383"
     CDS             complement(4970..5296)
                     /locus_tag="IT904_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAP3190218.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            5431..5772
                     /locus_tag="IT904_000384"
     CDS             5431..5772
                     /locus_tag="IT904_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAP3190219.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5807..6367
                     /gene="speG"
                     /locus_tag="IT904_000385"
     CDS             5807..6367
                     /gene="speG"
                     /locus_tag="IT904_000385"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HAP3190220.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(6370..7080)
                     /locus_tag="IT904_000386"
     CDS             complement(6370..7080)
                     /locus_tag="IT904_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAP3190221.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            7188..7493
                     /locus_tag="IT904_000387"
     CDS             7188..7493
                     /locus_tag="IT904_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAP3190222.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSSQDGAPAEPQ"
     gene            7692..10118
                     /locus_tag="IT904_000388"
     CDS             7692..10118
                     /locus_tag="IT904_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAP3190223.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVIWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYTCQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPKMTA
                     IRDGVRGKDKLDVPIKFIWNFAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMRYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            10179..12602
                     /locus_tag="IT904_000389"
     CDS             10179..12602
                     /locus_tag="IT904_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAP3190224.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEVPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            12613..13230
                     /gene="dmsB"
                     /locus_tag="IT904_000390"
     CDS             12613..13230
                     /gene="dmsB"
                     /locus_tag="IT904_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP3190225.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            13232..14086
                     /locus_tag="IT904_000391"
     CDS             13232..14086
                     /locus_tag="IT904_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP3190226.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGVGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLFSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            14129..14743
                     /gene="dmsD"
                     /locus_tag="IT904_000392"
     CDS             14129..14743
                     /gene="dmsD"
                     /locus_tag="IT904_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAP3190227.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGLQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14902..16194
                     /gene="clcB"
                     /locus_tag="IT904_000393"
     CDS             14902..16194
                     /gene="clcB"
                     /locus_tag="IT904_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAP3190228.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLTSLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(16147..16842)
                     /gene="bioD"
                     /locus_tag="IT904_000394"
     CDS             complement(16147..16842)
                     /gene="bioD"
                     /locus_tag="IT904_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP3190229.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16967..18187)
                     /gene="mlc"
                     /locus_tag="IT904_000395"
                     /gene_synonym="dgsA"
     CDS             complement(16967..18187)
                     /gene="mlc"
                     /locus_tag="IT904_000395"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAP3190230.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(18322..19215)
                     /locus_tag="IT904_000396"
     CDS             complement(18322..19215)
                     /locus_tag="IT904_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3190231.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPEPLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            19322..20575
                     /locus_tag="IT904_000397"
     CDS             19322..20575
                     /locus_tag="IT904_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3190232.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            20972..21307
                     /locus_tag="IT904_000398"
     CDS             20972..21307
                     /locus_tag="IT904_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAP3190233.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            21400..21483
                     /locus_tag="IT904_000399"
     CDS             21400..21483
                     /locus_tag="IT904_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190234.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            21583..22404
                     /locus_tag="IT904_000400"
     CDS             21583..22404
                     /locus_tag="IT904_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP3190235.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(22443..22772)
                     /gene="mdtI"
                     /locus_tag="IT904_000401"
     CDS             complement(22443..22772)
                     /gene="mdtI"
                     /locus_tag="IT904_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAP3190236.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(22759..23124)
                     /gene="mdtJ"
                     /locus_tag="IT904_000402"
     CDS             complement(22759..23124)
                     /gene="mdtJ"
                     /locus_tag="IT904_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAP3190237.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            23536..24570
                     /gene="tqsA"
                     /locus_tag="IT904_000403"
     CDS             23536..24570
                     /gene="tqsA"
                     /locus_tag="IT904_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAP3190238.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(24595..25983)
                     /gene="pntB"
                     /locus_tag="IT904_000404"
     CDS             complement(24595..25983)
                     /gene="pntB"
                     /locus_tag="IT904_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAP3190239.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(25994..27526)
                     /gene="pntA"
                     /locus_tag="IT904_000405"
     CDS             complement(25994..27526)
                     /gene="pntA"
                     /locus_tag="IT904_000405"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAP3190240.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            28050..28994
                     /locus_tag="IT904_000406"
     CDS             28050..28994
                     /locus_tag="IT904_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP3190241.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVIELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            29180..30562
                     /locus_tag="IT904_000407"
     CDS             29180..30562
                     /locus_tag="IT904_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3190242.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            30599..31321
                     /gene="folM"
                     /locus_tag="IT904_000408"
     CDS             30599..31321
                     /gene="folM"
                     /locus_tag="IT904_000408"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAP3190243.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(31318..31653)
                     /locus_tag="IT904_000409"
     CDS             complement(31318..31653)
                     /locus_tag="IT904_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAP3190244.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            31782..32501
                     /gene="rstA"
                     /locus_tag="IT904_000410"
     CDS             31782..32501
                     /gene="rstA"
                     /locus_tag="IT904_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAP3190245.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            32505..33806
                     /gene="rstB"
                     /locus_tag="IT904_000411"
     CDS             32505..33806
                     /gene="rstB"
                     /locus_tag="IT904_000411"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAP3190246.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNKATLIVEDDGSGIAPENREHIFEPFVRLDPSRGRSTGGCGLGLA
                     IVHSIALAMGGTVNCGTSELGGARFSFSWPLWHNIPQFTSA"
     gene            33882..34811
                     /gene="tus"
                     /locus_tag="IT904_000412"
     CDS             33882..34811
                     /gene="tus"
                     /locus_tag="IT904_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAP3190247.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(34808..36211)
                     /gene="fumC"
                     /locus_tag="IT904_000413"
     CDS             complement(34808..36211)
                     /gene="fumC"
                     /locus_tag="IT904_000413"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAP3190248.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEYNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(36354..38000)
                     /locus_tag="IT904_000414"
     CDS             complement(36354..38000)
                     /locus_tag="IT904_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAP3190249.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            38199..39374
                     /gene="manA"
                     /locus_tag="IT904_000415"
     CDS             38199..39374
                     /gene="manA"
                     /locus_tag="IT904_000415"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP3190250.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGVELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            39475..40983
                     /locus_tag="IT904_000416"
     CDS             39475..40983
                     /locus_tag="IT904_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAP3190251.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(41028..42293)
                     /gene="uidC"
                     /locus_tag="IT904_000417"
     CDS             complement(41028..42293)
                     /gene="uidC"
                     /locus_tag="IT904_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAP3190252.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(42332..43705)
                     /gene="uidB"
                     /locus_tag="IT904_000418"
     CDS             complement(42332..43705)
                     /gene="uidB"
                     /locus_tag="IT904_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAP3190253.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(43702..>44981)
                     /gene="uidA"
                     /locus_tag="IT904_000419"
     CDS             complement(43702..>44981)
                     /gene="uidA"
                     /locus_tag="IT904_000419"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP3190254.1"
                     /translation="TPNTWVDDITVVTHVAQDCNHASVDWQVVTNGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTEYDIYPLRVGIRSVAVKGEQ
                     FLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEML
                     DWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELI
                     ARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTD
                     TISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWKEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGI
                     FTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
BASE COUNT        11404 a        11513 c        10856 g        11208 t
ORIGIN      
        1 tggaaggcgc tgaacatcca cacaaggaga acactggcgg caatcagcat cacaatagcg
       61 ataatgaaac tggcgagacg gcagatcact caattaaggt gaacggtcat cacgaaatca
      121 catccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc
      181 ctgatgcccc gatcatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg
      241 gaccggaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcggaaca
      301 ccattaaatg gtggcttaag caatcacgcg aagcgcaatc tgccattatg accgatgaaa
      361 tcccgttaga tgatgcactg ttacaattgc gggaatttat cgacgaaaac tccggtgaat
      421 tttttgttca ggtttgggga aatggagcca acttcgacaa cacgattttg cgccgttcat
      481 acgaacggca ggggatcccc tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa
      541 tcgttgagct ggggaaagcc atagacttcg atgccagaac ggctattcca ttcgaaggtg
      601 agcgccataa tgcacttgat gacgcccgtt accaggcaaa atacgtttca gttatctggc
      661 aaaaactgat cccgagtcag gctgattctt aatgttcaac tgtcgccggt tgtgactggt
      721 attctgcaac cggcgctcgt ctgatgtaag agataaagaa atcgatgagc gaagtaatca
      781 tgattgtctc tcccggcaaa tgggtatccg aagagcagtt aattgcgctg aaaggaataa
      841 aaaaaggtac gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc
      901 atgtcgctca tgacggtatg ccatgggata acagtccatg cttttacaac ctggaagaaa
      961 ttgatcgctg gattgagcgc caggcatcag cgagaccaag acgtcatctt acttgactaa
     1021 aagccacact aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat
     1081 ggagggaaat tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaatctgggg
     1141 gttcctgaca cagcaaaaaa caggcgcgtt gcaggtgaac tacgctcctc tgtttgttac
     1201 gcaataaaaa ctggtgtttt cgactatgca aaacagtttc cctcctcacg caatctggaa
     1261 aaatttggtg aggcccgaca agatttaacc ataaaagaac tggctgaaaa atttctggca
     1321 ctgaaagaaa ctgaagtcgc caaaacatca ctcaacacat accgtgccgt catcaaaaat
     1381 atcctgagca taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg
     1441 gaggttcgta aagagctact gactggatac cagatcccca aaagtaacta tattgttaca
     1501 caaccagggc gatcggctgt aactgtaaat aattacatga caaatcttaa cgccgtgttc
     1561 cagtttggtg ttgataacgg ttacctgcca gataatccgc ttaaggggat ctcgccatta
     1621 aaggaatcaa gaaccattcc ggatcctctt tcgcgggagg aatttatccg tcttatcgat
     1681 gcgtgcagaa atcagcaagc aaaaaattta tggtgtgttt ctgtttatac tggcgttcgc
     1741 cctggtgagc tgtgtgcact tggatgggag gacatagatc tgaaaaatgg aacaatgatg
     1801 atcaggagaa atttagcaaa agaccgtttc acggtaccaa aaacacaggc gggaaccaat
     1861 cgggtcattc atcttattaa gccagcaatc gacgctctcc ggagccagat gacattaacg
     1921 agactgagca aagagcatat cattgatgtt cacctcagag agtatggcag aacagaaaaa
     1981 caaaaatgca cctttgtttt tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac
     2041 cattttaccg ttgactcaat aaggcagatg tgggacgcag cgataaaacg tgccggactc
     2101 cgccatcgaa aatcatatca gtcgagacat acttatgcct gctggtcgct gacagctggt
     2161 gctaacccgg catttatagc aaaccagatg ggccatgcag atgcgcaaat ggtatttcag
     2221 gtatacggaa aatggatgtc tgaaaacaat aatgcacagg tagctttgtt aaatacacag
     2281 ttaagcgagt ttgccccaac catgccccat aacgaagcaa tgaaaaatta atttaatatt
     2341 tatcaaatag ttaacatgca tgactcttga aatccataaa ttcaagcgca gtgcccagcc
     2401 atcccgatac tgctgctttc accaaatcct tagtgcttct ttcgtgtttt tctattgtca
     2461 taatggttat ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt
     2521 attcagaaaa agtgagtaag actttgcagc aatgtttttg atcctgttca aataaactaa
     2581 tggcatcagc aacatgctgg aaatcaaacg tatgggtaat taatttttct ggtttaatta
     2641 accctttact taaccagtcg ataacgaccg ggaatttatt tgcatttaag cgtgaagaga
     2701 aaatagagag ttcttttccg gtaattcctt gctgaatcac ttcagacggt tcactggaga
     2761 aacccatcaa tacaatacgt gccgctggag aagccagcgt tacggcctct ttcaggatag
     2821 aaggatgaca agccgcatca ataattaatg tcggcttgat gcctttttca gtgaaaatct
     2881 cgccaagcgg tgtctggctg ttattaatcg cccagtcagc cccgctctct ttcgcttttt
     2941 ccagtcgttc atcaatgcga tcggcaacaa tcacattttt aacgttatag acgcctttta
     3001 atacctgaac gatcgtcagg ccgattggac cggcaccata aaccagaacg gtatcatttt
     3061 cagtcggttg accatgtccg gttacgttag ccgcaatggt aaaaggttcg atcattaccg
     3121 catattgatc ggccactgct tcaggaattt tccacgcatt ttttgccgga accacggcat
     3181 attcactgaa accaccgtca gcgtgcacac ctaatacagc cagtgtcgta caaacgttcg
     3241 gtttacctat agagcacgga tagcaatgcc cacagctgac caccggatcg acagcaacac
     3301 gttcaccgac tctggcgctt tccacgcctt cacccactgc atcaatgacg ccaaagaatt
     3361 catgaccaat gacgcgcgga tatttcgcaa aaggattatg cccacgataa atatggctat
     3421 ctgaaccaca aattccggca agtttcactt ttactcgtac ttcacccgct gacggggtgg
     3481 gtatttcacg ttcgacaatc gccagttgat tcggtttttc aattaatatg cttttcatta
     3541 tcttactcct taccagttcc acagcgtgcc atcttccaga cgtgcgactg gtagataagc
     3601 aggttcatag ggatatttcg ccgccagctt ttcatcgaat tcgataccaa gacccggttt
     3661 gtctcccgga tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcgagcat
     3721 ttgttcggaa taacccatgt attcctggac accgaaattg gggacccaca gatcaaagtg
     3781 cagcgccgca gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac
     3841 ctgatacagc gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt
     3901 cagcgtggtg cggatataat cgatgagttg ctcttcaatc agttgtttgc agtcccagat
     3961 gctgttgaag acttcaccca ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa
     4021 gcattcctgg ttttccgcag gcgtcgggtc ttccatccag aacatgcgat aatcttcaat
     4081 gcttttacca aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa
     4141 atgttcatta aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag
     4201 gtatttctcc gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata
     4261 agccagacct ttacctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg
     4321 cacgcggatg gctttgaatc caagctcttg atgacgggca taatcatcca gagcttcatc
     4381 aatactgtga ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag
     4441 taactggtaa agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac
     4501 cgctgaaatg gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa
     4561 ctgccagata tcttcgatac ggtgcgcatc gcgaccaata agctgcggac aaaggtgatc
     4621 ctgcaaataa gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt
     4681 aataccgtcc tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa
     4741 aacttcagcc tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa
     4801 atctacgcaa ctaagctact accatacaag tataaagatc gaaaaaagcc ggagtgatca
     4861 caaaaaaagg cgtatatttg cgctgtgaat ggttgacaaa agatgaaata gaataccttt
     4921 tgtcagctga cacttcctct tatcttattg ataaaatgga tttatgttcc tacgtgcgcc
     4981 cccagcccgc aacaatgatc aacatgccgc aaagcgcaat caacgcaccc gtccagtcat
     5041 aaagagtcag tttcacgcca tccacaacgc gcagccacat caacgccgtg cagacataaa
     5101 cgccaccata agccgcgtaa acacgcccac tcgccgctgg atgcaacgtt aacaaccaga
     5161 caaacagcgc cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta
     5221 accacaacca gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta
     5281 acgttgtttt aatcatcttt gtctcttatt gacatcatgt atagttatag ggcgacataa
     5341 tatcatcaat ataaacaccc tcctggtacg ttttgcgtcc gcagtggatg gtgtagaatc
     5401 acctttaatc attcatacag ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc
     5461 ctgctcgcta ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact
     5521 aacaaactgg tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa
     5581 aaagagcaat ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa
     5641 gagtgggata aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac
     5701 atcaatgcct actgggagcc caatactttg cgctgcctgg accgtcgaac tggccgcgtt
     5761 attaccccct aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca
     5821 cagtgttaag ctacgcccgc tggagcgtga agatttacgc tatgtacatc aactcgacaa
     5881 taacgccagt gtgatgcgtt actggtttga ggaaccctac gaagcctttg ttgaactctc
     5941 tgatctgtat gataagcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga
     6001 cggcgaaaaa gccggtctgg tggagctggt ggaaattaac catgttcatc gccgcgcaga
     6061 atttcagata attatctccc cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa
     6121 attagcaatg gactatggct ttaccgttct caatctctat aagctgtatc tgatcgttga
     6181 taaagagaat gaaaaagcga ttcacattta ccgcaagctt ggcttttcgg ttgaaggtga
     6241 attgatgcac gagttcttta ttaatggtca atatcgtaat gccattcgca tgtgtatatt
     6301 ccagcatcag tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc
     6361 acaatagcat taataataat cgatcgtatt tttgatggtg taaacccgtt cgacggcggg
     6421 ttttactcct tcatcaacaa tgattagctg acagtccacc ggattagcgt gactgtcata
     6481 ttcacagctc tgttttacat taccaacccg ttgattattc agtaaagtaa ccgctgtgta
     6541 atctaatttt ttgatcggat ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt
     6601 acttttcgtg gttttaccca gcggataacc ctgatcatca tagcgatatt ccatctgcat
     6661 ttgtttgctg ctggctttaa tcacgaagcc attatcatcg gtttcccaac tcaccccggc
     6721 agaaggtaat tctgctagct ggcattttcc ctgtaaacgt actctcttct ccagcgtctc
     6781 ggcatcacgg taataattgg cgtccagtac cagagcgacc acggtattat tttccagatc
     6841 cagtaattcg agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt
     6901 cacttcacct tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg
     6961 atcaaaatcg aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc
     7021 tgtgcggtca catcctgtga ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac
     7081 aacagtcacc agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac
     7141 gttaaatgct atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa
     7201 cctgctgtgc cgcgcttctg ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat
     7261 cctgtgagcg catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa
     7321 gcagcttcac gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg
     7381 tcggccattg cgctaatgat acgcataaaa ttctctatac ccgcaccacc agcggtaacg
     7441 tctctgctcc cgcgcagtct agccaggatg gcgcgcctgc cgaaccgcag taacacatta
     7501 tcgactgaac gccggatatg acacatccgg cgatttgaac atacaacata atcccaccat
     7561 attactcata cccttctatt gatgtggatt aataattctt aacccaaaat gggtagactc
     7621 cctctattgt tagcgcgcta aatattcaat atataaacat ttatataacg ataaagaaca
     7681 gggagtgagt tatgtccaaa aatgaacgaa tggtgggcat cagccgcaga acactcgtta
     7741 aatctaccgc gataggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc
     7801 tacgcagtgc agcggcagcg gtacaacagg ccagcgagaa agtggtctgg ggtgcctgtt
     7861 ccgtcaactg tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgatct
     7921 gggtggaaac tgacaacacc ggcaacgacg agtatggcaa tcatcaagta cgcgcctgtt
     7981 tgcgcggtcg ctccatccgc cggcgtatta atcatcccga tcgcttgaat tacccaatga
     8041 aacgcgtggg taaacgcggt gaaggcaaat tcgaacggat tagctgggat gaagccctgg
     8101 atactatcgc cagtagtctg aaaaaaaccg tcgaacaata tggcaatgag gctgtatata
     8161 ttcagtactc ttcggggatt gttggcggca atatgacccg ctcttcgcca tcagcctcgg
     8221 cggtcaaacg cctgatgaac tgctacggcg gttcactcaa ccagtatggc tcctacagca
     8281 ctgcgcaaat ttcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca
     8341 cggatattga aaacagcaag ctggtcgtga tgtttggtaa caacccggca gaaacccgca
     8401 tgagcggcgg tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg aacgccaaaa
     8461 tgattgttat cgatccgcga tataccgata cggctgctgg tcgtgaagat gaatggcttc
     8521 ctattcgccc gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg
     8581 aaaatctcgt tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct
     8641 tacccgcaga tgcacccaaa aatggtcact ataaagccta tattcttggt gaaggtgacg
     8701 ataaaacagc gaaaacgccg cagtgggctt cgcaaattac cggtatcccg gtggaccgta
     8761 tcatcaaact ggcgcgtgaa attggcacag caaaacccgc ttatacctgc cagggctggg
     8821 ggccacaacg ccaggcaaac ggcgagctga ccgcacgcgc tattgctatg ttgcctattc
     8881 tgacgggcaa tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg acctatacca
     8941 ttaccataga gcgcctgccg gtgctggata atccggtcaa aacgtcaatt tcctgcttca
     9001 gctggacaga tgctatcgat catggcccca aaatgacggc aatccgcgac ggcgtccggg
     9061 gcaaagataa actggatgtg cccattaagt tcatctggaa cttcgcggga aataccctcg
     9121 ttaatcagca ttctgacatc aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg
     9181 aaatgattgt ggtcatcgaa aactttatga cctcatcggc aaaatatgcc gacattctgc
     9241 tgccagacct gatgaccgtt gagcaggaag atattattcc taacgactac gccggtaaca
     9301 tgagatatct cattttcctc cagcctgtca ccagcgaaaa attcgaacgc aaaccgattt
     9361 actggatcct gagtgaagtc gcgaaacgtc ttggacctga cgtctatcaa aagttcactg
     9421 aaggtcgcac gcaggaacaa tggttacaac atctgtacgc caaaatgctt gccaaagatc
     9481 cggcgttacc gtcttacgac gaactgaaaa aaatgggtat ctataagcgt aaagatccca
     9541 atggtcattt tgtcgcctac aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa
     9601 caccttccgg taagattgaa atttattcca gcaagctggc ggaaattgcc cgtacctggg
     9661 aactggaaaa agatgaagtg ataagcccac tgccagttta tgcctctacc tttgaaggct
     9721 gggactcccc tgaacgtaga accttcccac tgcaactgtt tggtttccat tacaaatccc
     9781 gtactcactc gacctacggc aacattgatg tcctgaaagc agcctgccgt caggaggtgt
     9841 ggatcaaccc tatagatgcg cagaaacgtg ggattgccaa cggcgatatg gtgcgggtgt
     9901 ttaaccatcg tggcgaagtt cggctaccag ccaaagtgac accacgtatt ctccctggag
     9961 ttagcgctat gggccaggga gcctggcacg aggccaatat gtctggcgat aaaatcgacc
    10021 atggcggctg tgtgaatacg ctaaccactc tgcgcccttc accactggcg aagggaaacc
    10081 cacagcacac taatctggtc gagatcgaaa aaatataacc cactacaacc ataaattctg
    10141 gcatgacatt ttgtttgaaa agcaataagt gagtaatgat gaaaatccat acgacagagg
    10201 cgctcatgaa ggctgaaatc agccgtagaa gtctgatgaa aacctccgca cttggcagtc
    10261 tcgcgctggc aagcagtgct ttcactctgc cattttccca aatggttcgg gcggcagagg
    10321 ttccggtaga agagaaagcg gtctggagtt cctgcaccgt taactgcggg agccgctgtc
    10381 tgttacgttt gcatgtgaaa gatgacaccg tgtactgggt ggagtctgat acgacaggtg
    10441 acgacgtcta cggtaatcat caggttcgcg cgtgtttacg cgggcgctct atccgccgac
    10501 ggatgaatca tcctgatagg ttgaaatatc ccatgaagcg cgtcggcaag cgcggtgaag
    10561 gtaaatttga acggataagt tgggacgaag ccctggatac catcagtgat aatcttcggc
    10621 ggatcctaaa agattacggc aatgaggctg tacatgtcct gtacggaaca ggtgtagatg
    10681 gcggaaacat caccaactca aacgtcccgt accgtctgat gaactcttgc ggtggttttc
    10741 tcagtcgcta tggcagctac agtaccgcac agatcagtgc cgcaatgagt tatatgttcg
    10801 gtgccaatga tggcaacagc ccggatgata tcgccaatac gaaactggtc gttatgttcg
    10861 gaaataaccc ggcagaaacg cggatgagcg gcggtggtgt cacttactac gtcgagcaag
    10921 cccgcgaacg ttcaaacgca cgcatgatcg tcatcgatcc acgttataac gacactgctg
    10981 ccgggcgtga agatgaatgg ctgcccattc gccctggcac cgatggcgca ctggcctgtg
    11041 cgattgcctg ggtactgatt actgaaaaca tggtcgatca gccatttctc gacaaatatt
    11101 gtgttggtta cgatgaaaaa acgctgcccg ccaacgcacc acgtaacgcg cattataaag
    11161 cctatattct gggcgaaggg cctgacggca tagctaaaac gccggaatgg gcagcaaaaa
    11221 tcaccagcat cccggcagaa aaaattatcc agttggcacg agagatcggt tcagcaaaac
    11281 ctgcttatat ttgtcagggt tgggggccac aacgacattc caacggcgag caaacatccc
    11341 gcgctattgc catgctttcc gttctcaccg gcaacgtcgg cataaacggc ggcaactcag
    11401 gcgtacgcga aggtagctgg gatctggggg tagaatggtt cccgatgctc gagaatcctg
    11461 ttaaaacgca gatttccgtc tttacatgga cagatgccat cgaccatggt acggaaatga
    11521 ccgcgacccg tgatggtgtt cgtggaaaag aaaaactgga tgtccccatc aagtttttat
    11581 ggtgctacgc cagtaacaca ttgatcaatc aacatggcga catcaatcac acccatgagg
    11641 tgcttcagga tgacagcaag tgcgagatga ttgttggcat tgaccacttc atgacggcct
    11701 cggctaagta ttgcgatatc ctgttgcccg acctgatgcc aacagagcaa gaagacctta
    11761 tctctcatga atctgcaggg aatatgggct atgtgatcct cgcccaaccc gcaacctcag
    11821 caaaatttga acgcaaaccc atctactgga tgctgagtga agtcgccaaa cgcttaggac
    11881 cagacgttta tcaaaccttt actgaaggtc gcagtcagca tgaatggatc aaatatctcc
    11941 atgcgaaaac gaaggaacgt aaccctgaga tgcccgacta cgaggagatg aaaacgaccg
    12001 ggatctttaa gaaaaaatgc ccggaagaac actacgtcgc tttccgcgca ttccgtgaag
    12061 atccacaggc aaacccgttg aaaacacctt cggggaaaat cgaaatttat tctgaacgac
    12121 tggcgaagat tgcagatacc tgggaattga aaaaagatga aattattcat ccccttcctg
    12181 cgtatacccc aggttttgat ggctgggacg atcccctgcg gaaaacctat ccactgcagt
    12241 taacgggctt ccattacaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac
    12301 agcaggcctg cccacaagag gtgtggatca accccattga tgctcaggca cgcggtatcc
    12361 gtcatggcga taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag
    12421 tgactccgcg tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg
    12481 atatgtttgg tgaccgggtc gatcacggcg gcagtatcaa tattctgacc tctcaccgcc
    12541 cttcaccgct ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt
    12601 aaggagtagc ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc
    12661 tgtaaaacct gcgaactggc gtgcaaagat tttaaagatc ttggcccgga agtcagtttc
    12721 cgccgtattt atgaatacgc tggcggcgac tggcaggaag ataatggcgt ctggcaccag
    12781 aacgtgttcg cttattatct ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca
    12841 aaagtctgcc ccagcggcgc aatgcataag cgtgaagatg gctttgtggt ggttgatgaa
    12901 gatgtttgta tcggctgccg ctactgccac atggcttgtc cgtacggcgc gccacagtac
    12961 aatgctgaaa aagggcacat gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg
    13021 aaacaaccca tatgtgtcga atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa
    13081 gagctgcgcc agaagcacgg cactcttgct gccgtcgcgc cactgccgcg cgcgcatttc
    13141 acaaaaccca atatcgttat caaacctaac gccaacagcc gcccgaccgg cgataccact
    13201 ggttatctgg ctaatccgga ggaagtgtaa aatgggaaat ggatggcatg agtggccact
    13261 ggttatcttt accgtattgg gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg
    13321 ctggttcgcc gccaaaaatg acgcagaccg tcagcgtatt gtccgtggca tgtttttcct
    13381 ctggctgttg atgggcgtcg ggtttattgc ctctgtcatg cacctgggat cgccactgcg
    13441 ggcgtttaac tcgctgaatc gaatcggagc ttctggtctg agtaacgaga ttgctgccgg
    13501 ttccatattt tttgccgttg gcggattatg gtggctggtg gcagtcatcg gtaaaatgcc
    13561 gcaagcgttg gggaaactct ggttactttt cagcatggcg ctcggcgtca ttttcgtctg
    13621 gatgatgacc tgcgtgtatc aaatcgacac cgtgccaacc tggcataacg gttacactac
    13681 gctggctttc ttcctgacgg tattgttgag cggtccaata cttgctgccg caattctgcg
    13741 ggcagctcgc gttacattta ataccacacc atttgccatc attagcgttc tggcgctgat
    13801 cgcttgtgcc ggggtgattg tgctgcaagg tttgtctctg gcgtctattc actcatccgt
    13861 gcaacaagcc agcgcactgg taccagatta cgcctcctta caggtctggc gtgtggtatt
    13921 gttatgcgca ggtcttggat gctggctttg cccgctcatt cgtcgccgtg aacctcacgt
    13981 tgccggactt attctggggc taatcctcat acttggcggt gagatgatcg ggcgtgtgct
    14041 cttttatggc ctgcacatga cagtaggaat ggctattgca ggttaacaaa ttggtgcggg
    14101 gcaacccgca catttcagga tgttaggaat gacccatttt tcacagcaag ataatttttc
    14161 tgtcgcggcg cgcgtgttgg gcgcgctgtt ttattacgct ccagagagtg cggaagccgc
    14221 acctctggtt gcggtactca ccagtgacgg ctgggaaact cagtggcctc taccagaagc
    14281 gtcattagcg ccgctggtga cggcttttca gacacagtgt gaagagactc acgcccaggc
    14341 ctggcagcgt ttgtttgtcg gcccgtgggc actgccgtct ccgccgtggg ggtcggtctg
    14401 gctggatcgc gaatctgtgc tgtttggcga ttcaacattg gcacttcgtc agtggatgcg
    14461 cgagaaaggc attcagtttg aaatgaagca aaacgaaccg gaagatcatt ttggttcact
    14521 gctgttgatg gctgcgtggt tggcggagaa tggtctccag acagaatgcg aagaattgct
    14581 ggcatggcac ctttttccgt ggtcaacacg ttttcttgat gtttttatcg aaaaagcaga
    14641 acaccctttc taccgtgcac tgggtgaact ggctcgctta acgctggcgc aatggcaatc
    14701 acaactgtta attcctgtcg cggttaaacc gctgtttcga taggatcacc gtaatattgc
    14761 cggatggtga cgattacgcg caatccggca ataataggtt acagtgtcac gtttttttat
    14821 ctcttaaagc acgcactgct tttgcggctg gcctcttttg ccgcaaaata gtcgcccgtg
    14881 tttcattgcc catttctgct catgcatcat ctacacatct atccggatct gcgcactatg
    14941 ttccgccgtc tgcttatcgc aacagtcgtc ggtattctcg cggcctttgc cgttgccggg
    15001 tttcgtcatg cgatgctgct actggagtgg ttgttcctca ataatgactc cggcagtctg
    15061 gtcaatgcag cgacaaacct ttccccctgg cgacggttgc taactccggc gctcggcgga
    15121 ctggcggcgg gtttgttgct gatgggctgg cagaaattta cccaacaacg ccctcatgcg
    15181 ccgaccgatt acatggaagc gttgcaaacc gatggacagt tcgattacgc agcaagcctg
    15241 gttaaatcgc ttgcctctct gctggtagta accagcggca gtgcaattgg tcgcgaaggt
    15301 gcgatgattc ttttagctgc ccttgccgcc tcctgttttg cccaacgttt tacgccacgc
    15361 caggagtgga aattatggat cgcctgtggg gccgcggcgg gaatggctgc ggcctatcgt
    15421 gccccgcttg ctggcagttt atttatagcc gaagtgctgt ttggcactat gatgttgacc
    15481 tctctcggcc cggtgattat ttccgccgtc gtggcattgc tggttagcaa tctgattaat
    15541 catagcgacg cgttactcta caacgtacaa ctctcagtga cggttcaggc tcgtgactat
    15601 gcgctgatta tcagtacagg tgtgctggca ggtctgtgcg gaccactgtt gttaacgtta
    15661 atgaacgcct gtcatcgtgg atttgtgagt ctcaaacttg cgccgccctg gcaactggca
    15721 ctaggcgggt tgatcgtggg tctgctttcc ctgttcacac ctgcagtgtg gggcaacggc
    15781 tatagcaccg tacaatcctt tttaaccgcc ccaccactgt taatgatcat tgccgggatc
    15841 ttcctttgta aactgtgtgc cgtgctggcg agtagcggtt ccggcgcacc cggtggggtc
    15901 tttacaccga cgctatttat cggtcttgcc attggcatgt tgtatggtcg tagcctggga
    15961 ttatggttcc ctgatggcga agaaattaca cttttactcg gattgaccgg gatggcgaca
    16021 ctgttggcgg cgaccacgca cgcgccgatt atgtcgacgt tgatgatatg tgaaatgacc
    16081 ggggagtatc agctactccc cggtttattg attgcctgcg taattgcgtc ggtaatttcg
    16141 cggacgttac accgtgactc tatctaccgc cagcacactg cgcagcatag ctaagcggat
    16201 gtattgcccc agttcacgct gttcggcgcg cggcagataa ggcagttcac caatgagcgg
    16261 tgccggaagt tttttaccca gcacatcaat gatttccgca taatgcgcca gtcctgggtt
    16321 gattcggtta gccacccagc caatgagcgg cagcccgtcg ttggcgatcg cctgagctgt
    16381 tagcagtgca tggttaatgc aaccttcctg aataccgaca accatcaaca ccggcagttg
    16441 ttcctgcact acccattcag agagtggacg caaatcattc atcagactgc gccagccgcc
    16501 agtcccttct accacgacat gatcgacttt ttcggtcagg tttgccaggc cgtttgaaat
    16561 gagggtgtaa ttgattgggc aactgtgcgc cacgctactt tcttcttcgc ttaacgcgat
    16621 aggattaact gcttcataag gcagttcgat ggttgaaaca ctctgcaaca ccagggcatc
    16681 tttattacgc agcccttcgg gtgtctcttt gctccccttc gctacgggtt tatatcccgc
    16741 aaccgttttt ccctgggagg ctaacgcttg tagcaatgcg cgggaaacca ccgttttccc
    16801 tacagaagtg tctgtaccgg taataaagaa acgcttcagc atcactaact ccaccgttat
    16861 gcttcacaaa tataaaccag gaaaataatt aaccttgaaa gtctaagtta tgctttcctg
    16921 gcccaaattg agatagcgca aattttggta gaacagttaa aaaatgttaa ccctgcaaca
    16981 gacgaatcaa caaagaaccg ttatacatcg cgtcttttac cagtgcagcg cctgccatcg
    17041 tgccctggtt agaaaactga gtactctcaa cgctgatgtg ctgactatac gcaggaaggg
    17101 cctgctgacg gatgctgtct gagatgaccg ggaagaggat atctgccgct ttacttaacg
    17161 gtgagccaat cagtattttt tgtgggttaa ataaattcac catgatggca agaatgcgcc
    17221 cgacatgcgc gcccaccccg gtaatgatgt cttttgccag tagatcgccg cgcaatgccg
    17281 cctgacacaa tgagtccacg gttaacggtt gtccatgtaa catcgagctc atggattgat
    17341 taagacgcag ctgtgccagc tcaagaatac tgtccacgct ggcgatggtt tcgaggcagc
    17401 cgtgattccc gcaataacag cgtttcccat acgggtcgac ctgtgtgtgg cctatttcca
    17461 cgagactact gctgcctgcg tgtagcagat gaccatcggt aatgacgccc gcccccacgt
    17521 tgtgatcgat aaccacctga atcacatcgc gcgccccgcg tgaggcacca aacaaggcct
    17581 ctgccatcgt ccatgcgctg atatcatgct gaatataaac cggaacgccg gtatgctgct
    17641 ccagcgcctc gccgagcggc atctctttta catcctcgta gaacggcatg cgatgtacaa
    17701 taccattttc cgtatcaata attcccggca aggttatggc aatcgaagtt agacgctcaa
    17761 gttttttctg gtggcggata aaaaactgat cgatatggga aataatacga tccagcaatg
    17821 gcaagtcatc ttttaacgcc agttcctgcg actcttccac caccagtttg ctgctcagat
    17881 cgcgcagagc aaggaaaatc tccccgcgac taatgcgcag agaaagatag tgccaggctt
    17941 cagtttcaac caccagcccc accgccggac ggccacggtt ccccgcttct ttgatttcca
    18001 gctcttgcac caggtgtgct tcgagcatct cacggacaat tttagtgata ctggcaggag
    18061 ccagttgcgc cagacgggaa agatcgatac gcgagactgg accaagctga tcaatcaggc
    18121 gataaaccgc gcccgcgttg gtctgcttta tttgatcaat gtgcccaggc tggttttcag
    18181 caaccaccgc atactcccta tattttcgcg ctccgaaata atctgtaggc tatggtgaag
    18241 cacttcaata cgtgtcgtca aatttttact taggcatgtg attaacagca catttttcgg
    18301 gctttttcgc tgaaatttcc ctcacctgag agcattcagc agatgaatac gaaagttacg
    18361 cgcagccgga ctttgttcat gatgtttcgg ccagaccaac cacatttcag aaaccgcatc
    18421 ctcttcagca atcggcaccc agcgcatttc gttgagctga acacgtttaa atgacgcagg
    18481 caaaattgaa acacccagac cggcggaaac cagaccgatg atggtcattg cctcgcccac
    18541 ctcctgagtg atgacgggcg tcaaatggta acgtcgcatc agcccgagaa tatcgtcata
    18601 cagccctgtc ccgacgtgcg gatcaaaaaa gacaaagggt tctttcgcca gttcagccag
    18661 cgttacattc gggttatttg ccaggggatg atcgtgcggg atcatcgcca taagcggttc
    18721 atggacgatg actgcgtgtt caagcggctc cggtaacgct gtgttacgca gcaatcccat
    18781 atccagcgtt ccttcaatga gcggagcgat ttgctcgcga gtgttcattt cgcgggtttg
    18841 taaatggaca tcaggataat cacggcgaaa cagcgataac gtatcggaca ccgcccgaat
    18901 aaaaggagcc gacgaagtaa aaccaatgcg caactccccc gcttcaccct gatgcagcct
    18961 ttcagcgcga gcggcagcgt catccaccat agacaggatt tgccgactat ctgcaagaaa
    19021 ctgttttcct gctgccgtca gcaatacact gcgattggtt cgtgccagca gtcgggcacc
    19081 aatttgttgc tccagcgcct gaatctgctg acttagcggc ggttgcgaaa tattcaggcg
    19141 ggcagcggcg cgcccgaaat gcagctcttc cgcaacagca acaaagtaac gcagatgacg
    19201 aagttcaata ttcatattta aaacatctta tttgagatta ttaatatatt agacagaaca
    19261 attcgatttt cctaccctat gtataagcct gatctacagg catatttagc aaggatttca
    19321 agtgagccgt actacaactg ttgatggcgc tccggcaagc gacactgaca agcaaagcat
    19381 ttctcagcca aatcaattta ttaaacgcgg tacgccgcaa tttatgcgcg tcaccctggc
    19441 gctgttctct gccggactgg caacatttgc acttctctat tgtgtgcagc ctatccttcc
    19501 ggtgctttcg caggagtttg gcttaacccc cgcgaacagt agtatttcac tgtccatttc
    19561 cacggcgatg ttggctattg gtttgctgtt tactggcccg ctatccgatg ccattggtcg
    19621 caaaccagtg atggtcacgg cgctactgtt ggcctccatt tgtacgttac tttcgacaat
    19681 gatgaccagc tggcacggca ttttgattat gcgcgccttg attgggcttt cgttaagtgg
    19741 cgtggcagct gttggcatga cttatcttag cgaggaaatc catcccagtt tcgtggcctt
    19801 ttcaatgggg ttgtatatca gcggcaactc aattggcggc atgagcggac gcttaattag
    19861 cggtgtcttc acggactttt tcaactggcg aattgctctg gcggcaatcg gttgtttcgc
    19921 gctggcctcg gcgttgatgt tctggaaaat cctccctgaa tcacgccatt ttcgcccgac
    19981 ttcgctgcgc cctaagacgt tgtttatcaa ctttcgtctg cactggcgtg accggggatt
    20041 accgttattg ttcgcagaag gctttttgct gatggggtcg ttcgtcacgc tgtttaatta
    20101 catcggctat cggttgatgc tctccccctg gcatgtcagt caggccgtgg ttggcttatt
    20161 atcgctggct tatttgaccg gtacatggag ctcacccaaa gccggaacca tgaccacccg
    20221 ctatgggcgt ggtccagtga tgttgttttc gacgggggtt atgctgtttg gtttactgat
    20281 gaccttattc agctcgctgt ggctgatctt tgccggaatg ttactcttct cagcaggatt
    20341 cttcgcagcc cactcagtag ccagcagctg gatcggcccc cgtgcaaaac gcgctaaagg
    20401 ccaggcctcc tcgctgtatc tgttcagtta ctatctgggg tcgagtattg ccgggacgct
    20461 gggtggtgtt ttctggcata actatggctg gaacggcgtc ggcgcattta ttgctctgat
    20521 gctggtcatt gctctgctgg tcgggacgcg tttgcatcgt cgtctgcacg cctgaaaaat
    20581 aagtccggac tgcggtaaat acccgtccgg acttattgcc agctcaaacc aacgttaata
    20641 gccatcctaa aatagacgaa gcgccagcca attcccgcag cgcatctagc gtcataagga
    20701 ttataagtac ccaaataaac ggattcattt tgctgtgtgt catttattac tgatgcgcag
    20761 ttattctacc gctttgtaag tagtaaaata gttaacccga tcaagactac tattattggt
    20821 agctaaattt tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt
    20881 gtacatatcg ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat
    20941 agttatttca acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat
    21001 gaaaaaagta ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc
    21061 tgcagagact gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccggcgaa
    21121 aactacacat cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa
    21181 aaagcatcat aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc
    21241 gaagaaacac gccaagaaac acagccatca gcaaccggca aaacctgctg cacaacccgc
    21301 agcgtaagtt ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta
    21361 agcgggtaac gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt
    21421 gagttcattc ttatcattct tattttatgc gcactgattg ccgcccgttt ttatctttcc
    21481 tgactgtagt catctgattt tactcccact ttcatcccgt cccgtctata gtatttatga
    21541 gggtttgctt ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct
    21601 gtagtgttgg gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt
    21661 gccaaatcgg caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca
    21721 gtgaatgaca cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc
    21781 aatttatgta cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta
    21841 ttgacacctc caaagggtaa agccgataaa gcagtggcgc tgcgttttgt gtcaaataaa
    21901 ggtctttggc gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag
    21961 cggttaaaag cagatgggga tggttggatt gtacctcccg cagccgcgcc gtgggacttc
    22021 ggattgattg tgctacgtaa tcccccttct ggcattacgc cgttgccgtt atttgaggga
    22081 gataaagccg cgcttaccgc cgcattaaaa gcggcaggtc gtaaagtgac tcaggcaggc
    22141 taccctgaag atcatctcga tacgttgtac agtcatcaaa actgtgaagt gactggctgg
    22201 gcgcaaacgt cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct
    22261 ctgatgttgc ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc
    22321 gcgaaagatc gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag
    22381 ctggatcaac tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt
    22441 catcaggcaa gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct
    22501 ttacgattta accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca
    22561 aacccgcccc acaatgcata agcgacagac aagtcgatcc ctttaacggc ttgagaaagc
    22621 gcactaaagg cagccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag
    22681 ccgtcagaaa atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc
    22741 caggcggcgt gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc
    22801 tttacgggta cctgatttta tcaacacaat cccggcgacc agggtggtta acccggcaat
    22861 tttcatcagc gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat
    22921 accgatacct tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga
    22981 gagaaatata tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc
    23041 actgacgctc gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc
    23101 taataaaatc caataaatat acattgtcct tctcctgcaa gagaattatt ttaattttcg
    23161 cttaattcag cgaaaccaaa gtaaagtggt caagccccgc gcatagcgca agctttggca
    23221 gaagaaagga ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg
    23281 aggtacgaag atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac
    23341 aattatccgc agtgttgctt ctcgtcatcg cggatgataa ttgtcctcgg tagttgaaca
    23401 cgcctgattt gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgat
    23461 cggtttgatt ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa
    23521 aaaacgggga caagcatggc aaagccgatc atcacgctca atggcctaaa aatcgtcatt
    23581 atgttgggaa tgctggtcat tattctctgc ggtatccgtt ttgccgccga gatcatcgtg
    23641 ccgtttattc tcgcattatt tattgctgtt attcttaacc cgctggtgca acacatggtc
    23701 cgctggcgtg tgccgcgtgt actggcggtg tcgattttga tgaccatcat cgtgatggcg
    23761 atggtgttgc tattagctta tctgggttcc gcgctcaacg agttgacgcg gacgttaccg
    23821 caatatcgca actctattat gacgccgctg caagcgcttg aaccgttgtt gcaacgcgta
    23881 gggattgacg tctcagttga ccagctggcg cattatattg atccgaacgc ggcgatgacg
    23941 ttgctcacca acttattgac gcagttatct aatgccatgt catcaatatt tttattgctg
    24001 ctgacggtgc tgtttatgct gctcgaagtg ccacaattgc ccggaaaatt tcagcaaatg
    24061 atggcgcgtc cggttgaagg gatggcggcg attcaacgtg cgattgacag tgtttctcat
    24121 tatctggtgc tgaaaacagc catcagcatc atcaccggcc tggtcgcctg ggcgatgctc
    24181 gccgcactcg atgttcgctt cgcttttgtc tggggattgc tggcctttgc gcttaattac
    24241 atcccgaata ttggttcagt cctcgcggca atccccccta tcgctcaggt actggtgttt
    24301 aatggcttct acgaagcgtt gctggtgctg gcgggatatc tgctgattaa tctggtcttc
    24361 ggcaatattc tggagccgcg tatcatgggg cgtgggctgg ggctttccac attggtggta
    24421 tttttgtcgt tgattttttg gggatggttg ttaggaccgg tgggtatgct gctttccgtg
    24481 ccgttgacaa ttattgtcaa aattgcgctt gaacaaacag cgggaggtca aagcatcgcc
    24541 gttctgttaa gcgatctcaa taaagagtga cggcctcagc agaggccgtc agggttacag
    24601 agctttcagg attgcatcca cgctggcttt ggcgtcacca aacagcatgt gggtgttttc
    24661 cttgaagaac agcgggtttt gcacaccagc atagccagtg ttcatcgaac gtttaaagac
    24721 aatcacgttc tgcgctttcc acacttccag cacaggcata ccagcaatcg gactcttcgg
    24781 atcatcctgc gccgccgggt taaccgtatc gttagcacca atcaccagta cggtatcggt
    24841 atcagcaaag tcatcattga tctcgtccat ttccagcacg atgtcatacg gtacttttgc
    24901 ttcagccagc aatacgttca tatgtccagg caaacgcccc gcgaccgggt ggataccgaa
    24961 acgcacatta ataccacgag cgcgcaattt ctcagtaatt tcagcgacag gatattgcgc
    25021 ctgcgcgact gccatgccgt accccggagt aatgatcact gaatgggagt ttttcagcag
    25081 ttccgctgtc tcttctgcgg tgatttcgcg gtgctcaccc acttcctgat catcgccagt
    25141 agaagagccg tcggtgccga aaccacccgc aataacgctg ataaaggaac ggttcatcgc
    25201 cttacacata atgtaagaaa ggatagcccc cgaagaaccg accagcgcac cggtcacaat
    25261 cagcaggtcg ttgctgagca taaagcccgc agccgcagcc gcccagccgg agtacgagtt
    25321 cagcatcgac accaccactg gcatatctgc accaccgatg gaggcgacta aatgccagcc
    25381 gaataccagc gcaattgcgg tcattatcag caatgccagc acttgcaggc cgacgctgtc
    25441 cgtgcgaaca aatacaatca gcagcaggaa ggaaacgacc agagccgcca ggttcatttt
    25501 gtgacggttt ggcagcatca atggtttaga cgaaatcttg ccacacagtt tgccgaacgc
    25561 caccaccgaa cccgtgaacg ttaccgcccc gatgaagata ccgaggaaca cttccgtcag
    25621 gtgaatattg accagaatcg gtgccattcc cgcgtcatga tgcagatagc tgttaaagcc
    25681 aaccagcact gccgccagac ccacgaagct atgcaggatc gccaccagtt ctggcatttc
    25741 ggtcatttca actttcttcg ccagacggat accaattgcc ccaccaatga ccatcgccag
    25801 caagatccag ccaacattac ccgtatccgg tccaaaaatg gttgcgatta acgcaatcgc
    25861 catcccggcg ataccgaagt tgttaccctg gcgagacgtt tcatgtttcg aaagaccggc
    25921 cagactgaag ataaacagga tcgcggcaac aatgtatgca gctgtaacta atcctccaga
    25981 catatgttac cccttaattt ttgcggaaca ttttcagcat gcgctgagtc acggtgaagc
    26041 caccgaaaat attaatgctg gctataagca ccgcgataaa actaaggaag ctaacccagc
    26101 cgccctggcc aatctgcaac agtgctccga caacaataat ccctgaaatc gcgttggtga
    26161 ccgacatcaa cggtgtatgc agcgcgtgcg atacattcca caccacgtaa taaccgacaa
    26221 cgcaggccag cgcgaaaacg gtgaagtgcc caaggaattc tttcggcgca acgcttgcca
    26281 tccagccaaa aagaatgatt gccagcgcca tcaacgcgta tttacgccac ggtgagcagg
    26341 tacatttttc ctcagttttc acttccggtg ccgctttttg tgccgcctgc ggctgagctg
    26401 atacctgaat cggcggtgcc ggccaggtaa tttcgcccgc acggatcacg gtcacgccgc
    26461 gaatcaccac atcatcaaaa tcaacagtga tattgccgtc tttctctttg cacaacagtt
    26521 tcagcagatt aacgaggttt gtgccgtaaa gctgtgagga ttgcgtcggc agacggcccg
    26581 gaagatcggt ataaccaatc actttgacac cattttccgt agtgaagatt tcacccggca
    26641 cggtgtattc acagttgccg ccgttttggg ctgccaggtc gacaatcaca ctgcccgcct
    26701 tcatggagtc aaccatttca cgggtaatta gcttcggcgc tggtttgcct ggaataagcg
    26761 cggtggtgac aatgatatcg acctcttttg cctgggcggc aaagagttcc atttccgctt
    26821 tgatgaacgc gtccgacatc actttggcat agccatcgcc gctgccagct tcctctttaa
    26881 aatccagctc gaggaattcc gcgcccatac tttgaacttg ttctttcact tccgggcggg
    26941 tgtcgaatgc acgcacaatc gcgccgagac tgtttgctgc gccaatggcg gccagacctg
    27001 caacacccgc accaatcacc atcacttttg ccggtggcac tttcccggcc gcagtaattt
    27061 gcccggtaaa gaagcgccca aattcatgtg ccgcttcaac aatggcgcga taaccggcga
    27121 tgttcgccat cgagcttagt gcgtccagcg attgtgcgcg tgagatacgc ggcacagagt
    27181 ccatcgccat cacggtcacg ttacgttccg caagtttttg cattaattcc ggattctgcg
    27241 caggccagat aaaactcacc agcgttgtcc caggattcag taacgcaatt tcatcatcta
    27301 acggcgcatt gaccttcaga atgatctctg actgccagac gctattccct tctacaattt
    27361 cagcgcccgc ttgcacaaac gctttatcgt caaaacttgc cagttgaccc gcgccgctct
    27421 ctaccgcgac ggtaaaaccc agtttcagca gctgttccac tgtttttggc gttgctgcaa
    27481 cacgggtttc attggttaac cgttctcttg gtatgccaat tcgcatgata ttcccttcca
    27541 tcggttttat tgatgatggt ttgcctgtgt caggagccac acaagctgct catgtacgag
    27601 ctaaatgtta ctccgttaaa ataaattagt aacaaacgcc ctataacgta ctgaaaatta
    27661 tgcctgtgat ctagcgccaa aaatcagtat ttcggcgtga actcgcaaaa tattaacgat
    27721 tcagccgtga tagtgggata aacaccttag aacgccggat aaagactgat aattgtcttc
    27781 gacggtcggg taaaacgaga catcgccccg gcacgaatca ctacttaaca ttaaattaac
    27841 ttatacaatt cagttgcttc agtagtaatg atgctgatac ggctgttttt taagcataga
    27901 cggtcatttg agcaggatta aaattggctt aaggaatgtg atatgaaaaa tgacgcagac
    27961 agttacaccg tttaaatgca ataatcagcc acgtttctcg ttaataacaa taccagtacc
    28021 tggtttgcgc aaggcgaagg attattttta tgaagcttaa gaacaccctc ctggcgtcgg
    28081 cactgctttc tgctacggca ttctccgtta acgcagcaac agaactgaca ccggagcaag
    28141 cggcagcggt taaacctttt gaccgtgtag tggttaccgg tcgttttaat gctattggcg
    28201 aagcggtgaa agccgtttct cgtcgcgcag ataaagaagg tgccgcctct ttttatgttg
    28261 tcgacacttc tgattttggt aacagcggta actggcgtgt ggtcgctgac ctctataaag
    28321 ccgatgctga aaaagcagaa gaaacaagta atcgcgtaat taacggtgtt atcgaactgc
    28381 cgaaagatca ggctgttctg attgaaccgt ttgacacggt caccgtccag ggcttctatc
    28441 gtagccagcc agaagtcaat gatgccatca ccaaagcggc aaaagcgaaa ggtgcctact
    28501 ctttctacat cgttcgtcaa atcgatgcca accagggcgg caaccagcgt attactgcat
    28561 tcatctataa aaaagatgct aagaaacgta tcgtccagag cccggatgtg atcccggcag
    28621 attccgaagc aggacgtgca gctctggctg ccggtggcga agccgcgaag aaagttgaga
    28681 tcccgggtgt tgcgactacc gcatcaccaa gttctgaagt cggtcgcttc tttgaaaccc
    28741 agtcatcaaa aggcgggcgt tacaccgtca cgctcccgga tggcactaaa gtcgaagaac
    28801 tgaacaaagc gaccgcagcg atgatggtcc cgttcgacag catcaaattc tctggcaact
    28861 acggcaacat gaccgaagtc tcctatcagg ttgcgaaacg tgccgcgaag aaaggtgcta
    28921 agtattacca catcacccgc cagtggcagg aacgtggtaa taacctgacc gtcagcgcag
    28981 atctgtataa ataactattc agttactcct ataaggcggc ttgatgccgc ctttcgcatt
    29041 tttagtcaca aatttccccg cgaccattgc atgcagacac atctcttcgt aaaatcccgc
    29101 gcttcagtgg caaactccat ttttatgcgt tttgccctaa tatttattct tcttatcacg
    29161 ttttaatcac tggatatcga tggaaaagaa actgggactg agcgcactca ccgcgctggt
    29221 attaagctca atgctgggcg cgggtgtttt cagtctgccg caaaatatgg cggcagttgc
    29281 cagcccggca gcactgctca tcggctgggg tattactggc gctggcattt tattgctggc
    29341 ctttgccatg ctgatcctca cgcgcattcg tcccgaactt gacggcggta tctttaccta
    29401 tgcccgcgaa gggtttggcg agctaatcgg cttttgttcc gcatggggat actggctgtg
    29461 cgcagtcatc gccaacgtct cctatctggt gatcgttttt tccgcgttaa gcttttttac
    29521 ggacacgccg gaattgcgcc tgtttggcga tggtaatacc tggcaatcga tagtcggtgc
    29581 atcggcttta ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa ccgctgccag
    29641 cattaacctg gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg ttgtgctggc
    29701 gatgatgatg ttcaaactgg ataccttcaa gctcgacttc accggacttg cacttggcgt
    29761 acccgtttgg gaacaggtga aaaacaccat gctgatcacc ctgtgggttt tcattggtgt
    29821 ggaaggtgcc gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg gcaaagcgac
    29881 actgctggcg gttctctccg ctctgggcgt ttacttgctg gtaacgctgc tttcgctggg
    29941 tgtggtggca cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg gtctgatggt
    30001 ggaaatgatg ggaccatggg gcgaaatcat catcgctgcc ggtttgatcg tttccgtttg
    30061 cggcgcgtac ttaagctgga ccatcatggc agcagaagta ccgttcctgg ccgccactca
    30121 taaagcattc ccgcgcattt ttgcgcgcca gaacgcacaa gctgcgccat ccgcctcact
    30181 gtggttgacc aacatctgcg tgcaaatttg tctggtactc atctggctga ccggttccga
    30241 ttacaacacg ttgctgacca tcgcctcaga aatgattctg gtgccctatt tcctggtcgg
    30301 cgcgttcttg ctgaaaatcg caacccgtcc actgcataaa gcggtaggtg tcggtgcctg
    30361 catttatggc ttatggttat tgtacgcctc tggtccaatg cacttgctgt tatccgttgt
    30421 tctgtatgca ccggggctgc tggttttcct gtacgcgcgc aaaacgcata cccatgataa
    30481 tgtactgaac cgtcaggaaa tggtgctgat tggcatgctg ttaattgcct cagttccggc
    30541 gacctggatg ctggtgggat aacgttcatc ccatcgttgg atagcaaagg agataacgat
    30601 gggtaaaacc cagcccttgc caatattaat tactggcgga ggtcgtcgca tcggcctcgc
    30661 cctcgcatgg catttcatta atcaaaagca accggtgatt gtcagctatc ggacacacta
    30721 tccagccatt gatggactga ttaatgcagg tgcgcagtgt attcaggctg atttttcgac
    30781 caacgacggt gtgatggcgt ttgccgatga agtactaaaa agcacccatg gtctgcgtgc
    30841 tattttgcat aacgccagtg cgtggatggc ggaaaaaccg ggtgcgccac tggccgacgt
    30901 actggcttgc atgatgcaga tccacgttaa taccccatac ctgctcaacc atgcgctgga
    30961 aagattactg cgtgggcacg gacacgccgc cagcgatatc attcacttta ccgattatgt
    31021 ggtggagcgc ggtagcgaca aacatattgc gtatgctgca agcaaagcgg cactggataa
    31081 tatgacccgc tcgtttgccc gcaagctggc accggaagtg aaagtgaatt ctattgcgcc
    31141 atcgctgatc ctgtttaatg aacatgatga tgccgaatat cgacaacagg cgctgaataa
    31201 atcactgatg aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg attacttact
    31261 taccagttgc tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc atctgcgtta
    31321 atgcagcttt atccagcata taatcaacac ccatgcgctt atcccccaac acgcaacaga
    31381 accaacaaaa gcggcgggca agcgcatcat tccggtgaaa taccacagcg acaccaggta
    31441 aacaaaatag ggaataatcg accacatact aaaaatgatg gttgcgcgta aggcttcaat
    31501 gccgcgttcg ctggcaacaa tataatgcgc gataagcgca aaggtcggga aaagtggaat
    31561 cagcccggcg atataataat ttttcgtttt tgctaaaaca ccaatcaaca gcactaccag
    31621 cgcaccgagc gcggctttga ttaccagccc catcttttta ccttaacact tccataacaa
    31681 gtcatcagta gaatacctga tgaaaacttg tttagaaacg attgatagta agtaaaaaca
    31741 gcgcggtgta ttgtgacgtt tttatatcta ccgtgaatgt tatgaacact atcgtatttg
    31801 tggaagatga tgcggaagtc ggttcactga ttgccgcgta cctggcaaaa catgatatgc
    31861 aggttaccgt agagccgcgc ggcgaccagg ccgaagaaac cattttgcga gaaaatccgg
    31921 atttggtgtt actcgacatc atgctaccag gcaaggacgg catgaccatt tgtcgtgatt
    31981 tacgcgcaaa gtggtctgga ccgattgttc ttctaacctc tctcgatagc gatatgaacc
    32041 acatcctggc actggaaatg ggtgcctgcg actatattct caaaacgacg ccccctgctg
    32101 ttttgctagc gcgtttacgt ttgcatttgc gtcagaatga gcaagccaca ctgaccaaag
    32161 gtcttcagga aacgtctctg actccctaca aagccctgca tttcggcacg ttgaccatcg
    32221 atcccatcaa ccgcgtagtc accctggcta acactgaaat ctcgctctcg acagctgatt
    32281 tcgaattatt gtgggaatta gctacccatg ccgggcaaat catggaccgc gatgcattgc
    32341 tgaaaaattt acgcggcgtc agttatgacg gactggatcg tagcgtggac gtggctattt
    32401 cgcggttaag aaaaaaacta ctcgataacg ccgcagaacc ttatcgcatt aaaaccgtgc
    32461 gtaacaaagg ctatcttttt gcgcctcatg catgggaata agcgatgaaa aaattgttta
    32521 tccagtttta cctgttattg tttgtctgct tccttgtgat gtctctgctg gttgggctgg
    32581 tgtacaaatt taccgccgaa cgcgcgggca aacagtcgct ggatgatttg atgaacagtt
    32641 cactgtatct gatgcgcagc gaattgcgtg agatcccccc acacgactgg ggtaagacgc
    32701 tgaaagagat ggatttaaat ctctctttcg atctgcgtgt cgagccactg agtaaatacc
    32761 atcttgatga tatttccatg caccgactgc gtggcggcga aattgtcgcc ctggacgatc
    32821 agtacacgtt tttgcagcgt atcccgcgca gccactacgt gctggcagtt ggtcctgttc
    32881 cttatcttta ttacctccat caaatgcgat tgctggatat cgccctgatc gcttttattg
    32941 ctatttccct cgcctttccg gtgtttatct ggatgcgtcc gcactggcag gatatgttaa
    33001 aactggaagc agcggcgcaa cgatttggcg atgggcatct caatgaacgt atccactttg
    33061 atgagggttc gagctttgaa cgacttggcg tcgcatttaa ccagatggcg gacaatatca
    33121 acgccttaat tgccagcaaa aaacagctta ttgacggtat cgctcacgaa ctgcgaacac
    33181 cgttagtgcg cctgcgttat cgactggaga tgagcgataa cctgagcgcc gccgaatccc
    33241 aggcgttgaa tcgtgatatc agtcaacttg aagctttaat tgaagagctg ctgacttatg
    33301 cccgactcga tcgcccacaa aacgagcttc atcttagcga accagacctg ccgttgtggc
    33361 tgtcaacgca tctggcagat attcaggcag taacgcccga taaaacggta cggataaaaa
    33421 cgctcatgca aggccattat gcggcgttgg atatgcgctt aatggagcgc gtgctggata
    33481 atttgctcaa taacgccctg cgctactgcc attcaacggt tgaaaccagc ctgctactgt
    33541 cggggaataa agcgacatta attgttgagg atgatggctc agggattgcc ccagaaaacc
    33601 gtgaacatat ctttgaacct tttgttcgcc tcgatcccag ccggggtcgc tcaaccggcg
    33661 gctgcgggct gggactggca attgtccact ctatagcact ggcaatgggc ggtacggtta
    33721 attgtggcac cagcgaactg ggtggtgccc gcttctcgtt tagctggcct ttatggcata
    33781 acatcccgca atttacctct gcttgacact acgcgcacgg tggtcaagtc accaccactg
    33841 tgctataaat taaggatgtt gtaactaaag tggttaatat tatggcgcgt tacgatctcg
    33901 tagaccgact caacactact tttcgccaga tggaacaaga gctggctgca tttgccgctc
    33961 atcttgagca acacaagcta ttggttgccc gcgtgttctc tttgccggag gtaaaaaaag
    34021 aggatgagca taatccgctt aatcgtattg aggtaaaaca acatctcggc aacgacgcgc
    34081 agtcgctggc gttgcgtcat ttccgccatt tatttattca acaacagtcc gaaaatcgca
    34141 gcagcaaggc cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat aacctttcgc
    34201 aagcagcgtt ggtcagtcat attcagcaca tcaataaact caagaccacg ttcgagcata
    34261 tcgtcacggt tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg catcgtcatt
    34321 tgccggggct gatcaccctt aatgcttacc gcacgctcac cgttctgcac gaccccgcca
    34381 ctttacgttt tggctgggct aataaacata tcattaaaaa tttgcatcgt gatgaagtcc
    34441 tggcacagct ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg
    34501 agtggcaaag aaaactggag cgagagtatc aggatatcgc tgccctgcca cagaacgcga
    34561 agttaaaaat caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg tacaaaggag
    34621 atcaaaaaca agtacaacac gcttgcccta caccgcttat tgcgctgatt aatcgggata
    34681 atggcgcggg cgtgccggat gttggggagt tgttaaatta cgatgccgac aatgtgcaac
    34741 accgttataa accacaggcg cagccgcttc gtttgatcat tccccggctg cacctgtatg
    34801 ttgcggatta acgcccggct ttcatactgc cgaccatctg ttctggccgt acccagctgt
    34861 caaactcggc ttcgctaaga taccccagcg caagggccgc agcttttaag gtcagccctt
    34921 ctttatgcgc ttttttggcg atctccgcgg ctttgtcata accaatgtgg gtgttaagcg
    34981 cagtcaccag catcagcgat tcattgagta attgattgat tcgctcacga ttcggttcaa
    35041 tacccactgc gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag cgcaccgatt
    35101 gcaggaaatt gtggatcacc attggacgga agacgttcag ttcaaagtta ccggaagcgc
    35161 cccccatgtt gatcgccacg tcgttcccca tcacctgaca gcagagcatg gttaatgcct
    35221 cacactgtgt tggattcact ttccccggca tgattgagct gcccggctca ttttccggga
    35281 ttgagatttc accaattccg cagcgcgggc cagaggccag ccagcggaca tcattggcga
    35341 ttttcatcag tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc agagcatcac
    35401 aggtcgccag cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag gtaatgactg
    35461 ccagttcatc tgctacgcga cgcgcatact ccggatgggt atttagtcca gtacccaccg
    35521 ctgtaccgcc aagagccagt tccgctacgt gaggcaggct gtattcgata tgtttgagat
    35581 tatactcgag catcgctacc cagccggaaa tctcctgccc cagtgttagc ggcgtggcgt
    35641 cctgcaagtg ggttcgaccg attttgacga tatcggcaaa tgcacgcgat ttttcactca
    35701 gcgtctgtgt cagggtttta agctgcggaa tgagttgctt gcgcagcgcc agcagcgccg
    35761 caacgtgcat cgccgtcgga aagacatcgt tggaactttg gcttttgttc acgtcgtcgt
    35821 tagggtgaac tttacgttcc atcccgcgca cgccgccgag taattcactg gcccggttag
    35881 ccagcacttc gttcatgttc atgttacttt gcgtgccgga gccggtctgc cagatagcca
    35941 gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc
    36001 tcgctttctc ttcagacaac aagcctaaat cttcattaac ttttgccgct gcgcgcttgg
    36061 ttagcgccag cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg cggaaatgct
    36121 ccagcgagcg ttgagtttgt gcgccccaca gcttatctgc cgggacatca atcgccccca
    36181 tcgaatcttt ttcgctgcgt actgtattca tggcctgctc ctcacctgtt taattttttc
    36241 tttctgtttt gctttcgtta agcaactttt agctcactta ttatttacta tttgataaca
    36301 aatgtttggt ctttcgtgcc atgtaaaaaa accgccccga agggcggctc tgtttatttc
    36361 acacagcgag tgcactgtgt gagttgtatc tgctggaaga agtcatttcc tttatcatcc
    36421 acaaggataa acgccgggaa atcttccact tcaattttcc agatggcttc cattcccagt
    36481 tccggatatt caacacattc caggctctta atacttccct gcgccaatac agcggccgga
    36541 ccaccgatac tgccaaggta gaagccgccg tgttttttac aggcatccgt cacctgctgg
    36601 ctgcggttgc ctttcgccag catgatcata cttccgccct gcgcttgcag ttgatcgaca
    36661 taagaatcca tccgtccggc ggtcgttggg ccaagagaac cggaggcata accttccggc
    36721 gttttggccg gacccgcgta gtaaatcgga tgatctttaa tgtactgcgg cagcccttca
    36781 ccgttatcca tccgctcttt cagtttggcg tgagcaatat cacgaccgac gataatcgtg
    36841 ccgttaagcg ataagcgtgt agaaacggga tactgcgaca actgtgcgag gatctctttc
    36901 atcggacggt taaggtcaac gcgcaccgct tcgccttctc ccgctttacg cagctcttca
    36961 gggatatatt tacctggatt atgttccagt ttttcgatcc agatcccctg acggttgatc
    37021 ttcgctttga tattacggtc agcagagcag gaaacaccca taccaaccgg acaggatgcg
    37081 ccgtgacgtg gcaggcgaat cacgcgtatg tcgtgagcga agtatttacc accaaactgc
    37141 gcacccagac cgagattttg cgcttcgatc agcaattctt tttccagttc cacatcgcgg
    37201 aatgcctgac cgtgctcatt cccttccgtt ggcagttcgt catagtattt agcggaagcc
    37261 agtttcactg ttttaaggtt agtttctgca gaagttccac caattacgaa cgcaatatga
    37321 tacggaggac aggccgccgt acccagcgtg cgcatcttct caaccaggta atttttcagt
    37381 ttccccggcg ttagtaacgc tttggtttcc tgatagagat acgtcttgtt tgccgaacca
    37441 ccacctttgg cgatacagag gaatttgtac tcgtcgccat caacggcata aagatcgatc
    37501 tgcgctggca gattggtgcc ggtattcacc tctttataca tatccagcgg cgcgttttgc
    37561 gagtagcgca aattatcttc gatataagtg ttatagacac cgcgcgccag cgccgcttca
    37621 tcacagccac cggtccatac acgctgccct tttttaccaa caataatcgc agtgccggta
    37681 tcctgacagg ttggcagaac gcctttcgcc gcgatgtcgg agttacgcag gaattgcagc
    37741 gccacatatt tatcattttc gctggcctcc gggtcacgca gaatgtcggc cacctgttgt
    37801 tggtgagccg gacgcagcat aaacgacgca tcatgaaacg cctgacgcgc caacagagtt
    37861 aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa attcagatac gctaacgtgt
    37921 tcgctggtta gcaggtaata ctcagtatca tcttttttga gtggaaaagg agcctgataa
    37981 tgaaagggtt tgtttgacat tgttctctca cttactgcct ggtttggtta tgctctgggc
    38041 gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc tacacacttt tttaacaaaa
    38101 actgagacta gtacgacttt ttgcggatcc aggttacttc ccgtaggatt cttgctttaa
    38161 tagtgggatt aatttccaca ttaaaacagg gattgatcat gcaaaaactc attaactcag
    38221 tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt atggaaaatc
    38281 cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc agttcacgag
    38341 tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt gattgagagt gataaatcga
    38401 ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg ttcaaagtat
    38461 tatgcgcagc acagccgctc tccattcagg ttcatccaaa caaacgcaat tctgaaatcg
    38521 gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt aactataaag
    38581 atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt
    38641 ttcgtgagtt ttccgagatt gtctccctac tccagccagt cgcaggtgca catccggcaa
    38701 ttgctcactt tttacaacag cctaatgccg aacgtttaag cgaactgttc gccagcctgt
    38761 tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gcgctcgaca
    38821 gtcagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac ccggaagaca
    38881 gcggcctgtt ctccccgctg ttactgaatg tggtgaaatt gaatcctggc gaagcgatgt
    38941 tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga
    39001 actccgataa cgtgctgcgt gcgggtctga cgcctaagta cattgatatt ccggaactgg
    39061 ttgccaatgt gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac
    39121 aaggtgtaga actggacttc ccgattccag tggatgattt tgccttctcg ctgcatgacc
    39181 ttagtgataa agaaaccacc attagccagc agagtgccgc cattttgttc tgcgtcgaag
    39241 gcgatgcaac gttgtggaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt
    39301 ttattgccgc caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt
    39361 acaacaagct gtaagagctt actgaaaaaa ttaacatctc ttgctaagct tattaaaggc
    39421 ttataacacc ttcaggcggc cagtccgcct gatttcattt tatggataat cattatgaat
    39481 aaatcgctgg tagcggtagg cgtcattgtt gcgctaggcg tagtctggac aggcggcgca
    39541 tggtatacag gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg
    39601 caactcaaac tgacagctcc tgaatccaac ctggaagtga gttatcaaaa ctatcatcgc
    39661 ggcgtattca gcagccagtt gcaactgttg gtgaaaccca ttgccgggaa agaaaatccg
    39721 tggattaaaa gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg
    39781 cttgcccagc ttaaaaaact gaacctgatc ccgtcgatgg catcaattca aaccacgctg
    39841 gttaataacg aagtaagcaa accactgttt gatatggcaa aaggtgaaac gccttttgag
    39901 attaactcgc gcattggtta cagcggtgat tccagttccg atatttcgct caagccactg
    39961 aattacgagc aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca gttaaatgct
    40021 gacagagacg gcaaagccat ctccctttcc ggggaggcgc aaagtggtcg gatagacgca
    40081 gttaacgaat acaaccagaa agtgcagttg acctttaata atctgaaaac cgacggttcc
    40141 agcacgctgg caagttttgg tgagcgcgta ggaaaccaaa aactgtcact ggaaaaaatg
    40201 accatttcag tggaaggcaa agaactggca ctgctggaag gcatggagat cagcggtaaa
    40261 tcggatctgg tcaatgacgg taaaacgatc aatagccaac tggattactc gctaaacagc
    40321 ctgaaggtac agaatcagga tctgggcagc ggcaagctga ctttaaaagt cggccagatt
    40381 gatggtgaag cctggcatca gtttagccag caatataacg cgcaaactca ggcgctgctg
    40441 gcacagccag aaattgccaa caatcccgaa ctttatcagg agaaagtgac ggaagccttc
    40501 tttagcgccc tgccgctgat gttgaaaggc gatccggtga ttactatcgc gccgctaagc
    40561 tggaaaaaca gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa agatccggca
    40621 acgactaaag aagcgccgca aacgctggcg caggaagtag atcgttcggt taaatctctg
    40681 gatgcgaaac tgaccattcc ggtggatatg gcaactgagt ttatgactca ggtagcgaag
    40741 ctggaaggtt atcaggaaga tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca
    40801 tcagcaatgg ggcagatgtt ccgtctgacc accttgcagg acaataccat caccaccagc
    40861 ctgcaatata ctaacggtca gataacgtta aacgggcaga aaatgccact ggaagatttc
    40921 gttggtatgt ttgcaatgcc ggcattaaat gttccggtcg tacccgctat tccgcagcag
    40981 taattcacag acaaaacgcc ggagattttt ctctccggcg ttatttttta cttcagcata
    41041 aagtcatact gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg
    41101 ccatcgcgct tttcccgccc tttatcggca gcaagaaaga aattcatccc ttgtaacggc
    41161 ccttcgctga cccgatagtt cataccacct aagatacccg tgccttcaat ccgcacatct
    41221 cgtttactgg attcatagcc gcgagtcaca atcggtgcaa atgtcagcgt aaattgcggc
    41281 gttaaacgat agttgacgcc caattgccag gactgcattc cttcacggtt acgatcaaga
    41341 cttaatgaaa agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc
    41401 aaataaccac cactgccaaa tacgcttccc cacggcgcat tccaggtcaa ttgtccacta
    41461 accaacgccg tttcattggg ctggctggta ttacgactta aaccttcgag ctgcgcgtaa
    41521 aagccgagca actccccttt caacgtataa ttcgggtcga gcggtatgct tttctcgaga
    41581 atactttgta tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg
    41641 gtatcatcat gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc
    41701 gtctggctgt tatacacccc ttcatcacga ataccggttg ttgaatcagt ccataaaaat
    41761 actcgtgaac gccacattcc ccacagatca atatttggtg ttaaagccca ataacctaat
    41821 gctccttcag aaactgtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga
    41881 taaggtaagc taccataact ataatccgtc ccgaagcgac ctaatttaag caggctattt
    41941 tcactgggtt ttatttttac tgcccccaga gcaaagccaa aacttttatc accatcaaga
    42001 taccaccggg tactcatatc agcacgagca cctaatttat aaacataata cgccccgcct
    42061 tcaacgccaa taatattgga ataataacca ctattgaaat cgagcaatcc tccctgtacc
    42121 catgcgtaaa tatctcttcc cgcgccagca ctgggcttat ccgccctgcg caattcgttt
    42181 ttcagtctga tgcgtagtcc cgcttcatcg tcagccagtt gcgccgcata agcagaggta
    42241 aggccagagg cagccgtcag gcaaataacg gccatggcca ctatttttct catagttgca
    42301 cctttgatgt tctgattatt tttattgaat attaattagt gatatcgctg attaattgct
    42361 gctgcacttt tttacgatta tcaatttcaa ccacgatttc tttgaatttt ttatccgtga
    42421 gcggataaaa ccagataata acgaatgcca gtagcataaa tccgcaaggt actaaggcaa
    42481 ttgatgtgcg gatgcccata ataacttccg gcgtttgcac ctgattggcg atatatccgc
    42541 ttaaccccaa aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa
    42601 atgagaatag tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt
    42661 ctacggtatc agcttccagc gcccacatca cggtcatggt aacgccctga ccaattgaag
    42721 cgatggccaa cgcaaccaac gccaccggca gtgaccagac ggaaacccag aagaacagca
    42781 gataaccgca ggttcccagc aaagcgccaa tcaggaaggt attcttttta ccgatcctcg
    42841 cgaccatccc cggcaccagc ggtgccgatg ccacagtacc aaccaggttt tgcaccagta
    42901 ccagcacagt gaacagcccg gtatcattta acacatagcg cacgtagaac aacgacgagg
    42961 cgctgaccgc aaaggtcgaa atcagcacac acagcgcacc gatgcacaac ataaacagcg
    43021 ggcgattccg tttcagggtt tgcagactga tattcaatga cggctgcgca acgatacgta
    43081 ccacattctc acgcgtcgat ttgaagcaga tgaagtaaag caccattccg gcaatcgcca
    43141 gcacaattgt ccagaaatgg tataccgaca ccatctcttc cgggctggag ttcttaatgc
    43201 tcggtcctat cagaaatgcc aggcagacaa aggtcaatga agcggcaatc ccacgagccg
    43261 cgcccagacg ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaagt gaaccataag
    43321 gaatattcac caggctgtag caaagcccga ggcccatgta ggtcaaatat gcatacacca
    43381 ctttgctacc atggctccag tcggtcggca cccagaatac cagcacgctg aagatcatta
    43441 acggcgcagt accgaagagt aaaaacgggc ggaattttcc ccagcgggta ttcacactgt
    43501 ccaccactcg tccggcaaag acgtcggcga aggcatcgaa tacccgcacc agtaacagca
    43561 tggtgcccgc cgcagcggca ccgacgccag cgacgtcggt gtagtaactc aacaggaaga
    43621 gcgcccccat tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag ccgacgatgg
    43681 tgcgccagga gagttgttga ttcattgttt gcctccctgc tgcggttttt caccgaagtt
    43741 catgccagtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc ggtcgcgagt
    43801 gaagatccct ttcttgttac cgccaacgcg caatatgcct tgcgaggtcg caaaatcggc
    43861 gaaattccat acctgttcac cgacgacggc actgacgcga tcaaagacgc ggtgatacat
    43921 atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg agtgcagccc
    43981 ggctaacgta tccacgccgt attcggtgat gataatcggc tgatgcagtt tctccttcca
    44041 ggccagaagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc tttggacata
    44101 ccatccgtaa taacggttca ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg
    44161 agcgtcgcag aacattacat tgacgcaggt gatcggacgc gtcgggtcga gtttacgcgt
    44221 tgcttccgcc agtggcgcga aatattcccg tgcaccttgc ggacgggtat ccggttcgtt
    44281 ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgctatca gctctttaat
    44341 cgcctgtaag tgcgcttgct gagtttcccc gttgactgcc tcttcgctgt acagttcttt
    44401 cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga cagcagcagt
    44461 ttcatcaatc accacgatgc catgttcatc tgcccagtcg agcatctctt cagcgtaagg
    44521 gtaatgcgag gtacggtagg agttggcccc aatccagtcc attaatgcgt ggtcgtgcac
    44581 catcagcacg ttatcgaatc ctttgccacg caagtccgca tcttcatgac gaccaaaacc
    44641 cgtaaagtag aacggtttgt ggttaatcag gaactgttcg cccttcactg ccactgaccg
    44701 gatgccgacg cgaagcgggt agatatcata ctctgtctgg cttttggctg tgacgcacag
    44761 ttcatagaga taaccttcac ccggttgcca gaggtgcgga ttcaccactt gcaaagtccc
    44821 gctagtgcct tgtccagttg caaccacctg ttgatccgca tcacgcagtt caacgctgac
    44881 atcaccattg gtcaccacct gccagtcaac agacgcgtgg ttacagtctt gcgcgacatg
    44941 cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg g
//
LOCUS       DACAPB010000008        44373 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.008, whole genome shotgun sequence.
ACCESSION   DACAPB010000008 DACAPB010000000
VERSION     DACAPB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.008"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            181..2880
                     /locus_tag="IT904_000420"
     CDS             181..2880
                     /locus_tag="IT904_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAP3190255.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFLGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            2978..4540
                     /gene="casA"
                     /locus_tag="IT904_000421"
     CDS             2978..4540
                     /gene="casA"
                     /locus_tag="IT904_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HAP3190256.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4537..5073
                     /gene="casB"
                     /locus_tag="IT904_000422"
     CDS             4537..5073
                     /gene="casB"
                     /locus_tag="IT904_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HAP3190257.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            5085..6140
                     /gene="cas7e"
                     /locus_tag="IT904_000423"
     CDS             5085..6140
                     /gene="cas7e"
                     /locus_tag="IT904_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HAP3190258.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKVLAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTDYASFD
                     VMNKQGSMKDVLDFICA"
     gene            6151..6897
                     /gene="cas5e"
                     /locus_tag="IT904_000424"
     CDS             6151..6897
                     /gene="cas5e"
                     /locus_tag="IT904_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HAP3190259.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGG
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDRFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            6879..7529
                     /gene="cas6e"
                     /locus_tag="IT904_000425"
     CDS             6879..7529
                     /gene="cas6e"
                     /locus_tag="IT904_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HAP3190260.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFTPELSTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSWQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7526..8449
                     /gene="cas1e"
                     /locus_tag="IT904_000426"
     CDS             7526..8449
                     /gene="cas1e"
                     /locus_tag="IT904_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HAP3190261.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDLVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8446..8739
                     /gene="cas2"
                     /locus_tag="IT904_000427"
     CDS             8446..8739
                     /gene="cas2"
                     /locus_tag="IT904_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HAP3190262.1"
                     /translation="MSMIVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8836..9169
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8836..8864
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(9251..10288)
                     /gene="iap"
                     /locus_tag="IT904_000428"
     CDS             complement(9251..10288)
                     /gene="iap"
                     /locus_tag="IT904_000428"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAP3190263.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10540..11448
                     /gene="cysD"
                     /locus_tag="IT904_000429"
     CDS             10540..11448
                     /gene="cysD"
                     /locus_tag="IT904_000429"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAP3190264.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11450..12877
                     /gene="cysN"
                     /locus_tag="IT904_000430"
     CDS             11450..12877
                     /gene="cysN"
                     /locus_tag="IT904_000430"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAP3190265.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12877..13482
                     /gene="cysC"
                     /locus_tag="IT904_000431"
     CDS             12877..13482
                     /gene="cysC"
                     /locus_tag="IT904_000431"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAP3190266.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRYFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13532..13855
                     /locus_tag="IT904_000432"
     CDS             13532..13855
                     /locus_tag="IT904_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAP3190267.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            14049..14360
                     /gene="ftsB"
                     /locus_tag="IT904_000433"
     CDS             14049..14360
                     /gene="ftsB"
                     /locus_tag="IT904_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAP3190268.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14379..15089
                     /gene="ispD"
                     /locus_tag="IT904_000434"
     CDS             14379..15089
                     /gene="ispD"
                     /locus_tag="IT904_000434"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP3190269.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            15089..15568
                     /gene="ispF"
                     /locus_tag="IT904_000435"
     CDS             15089..15568
                     /gene="ispF"
                     /locus_tag="IT904_000435"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAP3190270.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15565..16614
                     /gene="truD"
                     /locus_tag="IT904_000436"
     CDS             15565..16614
                     /gene="truD"
                     /locus_tag="IT904_000436"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAP3190271.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16595..17356
                     /gene="surE"
                     /locus_tag="IT904_000437"
     CDS             16595..17356
                     /gene="surE"
                     /locus_tag="IT904_000437"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAP3190272.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17350..17976
                     /gene="pcm"
                     /locus_tag="IT904_000438"
     CDS             17350..17976
                     /gene="pcm"
                     /locus_tag="IT904_000438"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAP3190273.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            18116..19255
                     /gene="nlpD"
                     /locus_tag="IT904_000439"
     CDS             18116..19255
                     /gene="nlpD"
                     /locus_tag="IT904_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAP3190274.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            19318..20310
                     /gene="rpoS"
                     /locus_tag="IT904_000440"
     CDS             19318..20310
                     /gene="rpoS"
                     /locus_tag="IT904_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAP3190275.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20404..21768)
                     /locus_tag="IT904_000441"
     CDS             complement(20404..21768)
                     /locus_tag="IT904_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAP3190276.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLLGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(21857..22633)
                     /locus_tag="IT904_000442"
     CDS             complement(21857..22633)
                     /locus_tag="IT904_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP3190277.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNIRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(22638..23276)
                     /locus_tag="IT904_000443"
     CDS             complement(22638..23276)
                     /locus_tag="IT904_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP3190278.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(23273..24535)
                     /locus_tag="IT904_000444"
     CDS             complement(23273..24535)
                     /locus_tag="IT904_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAP3190279.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDDAFFSRAQREFLS"
     gene            complement(24532..25440)
                     /locus_tag="IT904_000445"
     CDS             complement(24532..25440)
                     /locus_tag="IT904_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP3190280.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIAIALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIASGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            25606..26403
                     /locus_tag="IT904_000446"
     CDS             25606..26403
                     /locus_tag="IT904_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP3190281.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(26454..27110)
                     /gene="pphB"
                     /locus_tag="IT904_000447"
     CDS             complement(26454..27110)
                     /gene="pphB"
                     /locus_tag="IT904_000447"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP3190282.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTPVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKLHHLPHIIEIINDNIKYVIAHADYPGDEYQFGKEIVESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(27216..29777)
                     /gene="mutS"
                     /locus_tag="IT904_000448"
     CDS             complement(27216..29777)
                     /gene="mutS"
                     /locus_tag="IT904_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP3190283.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            29853..29987
                     /locus_tag="IT904_000449"
     CDS             29853..29987
                     /locus_tag="IT904_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190284.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            30064..30417
                     /locus_tag="IT904_000450"
     CDS             30064..30417
                     /locus_tag="IT904_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAP3190285.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPHE"
     gene            complement(30454..32532)
                     /gene="flhA"
                     /locus_tag="IT904_000451"
     CDS             complement(30454..32532)
                     /gene="flhA"
                     /locus_tag="IT904_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAP3190286.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLDAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVALEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32606..33616)
                     /gene="hypE"
                     /locus_tag="IT904_000452"
     CDS             complement(32606..33616)
                     /gene="hypE"
                     /locus_tag="IT904_000452"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAP3190287.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(33613..34734)
                     /gene="hypD"
                     /locus_tag="IT904_000453"
     CDS             complement(33613..34734)
                     /gene="hypD"
                     /locus_tag="IT904_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAP3190288.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARDADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(34734..35006)
                     /gene="hypC"
                     /locus_tag="IT904_000454"
     CDS             complement(34734..35006)
                     /gene="hypC"
                     /locus_tag="IT904_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAP3190289.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(34997..35869)
                     /gene="hypB"
                     /locus_tag="IT904_000455"
     CDS             complement(34997..35869)
                     /gene="hypB"
                     /locus_tag="IT904_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAP3190290.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(35873..36223)
                     /gene="hypA"
                     /locus_tag="IT904_000456"
     CDS             complement(35873..36223)
                     /gene="hypA"
                     /locus_tag="IT904_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP3190291.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            36435..36896
                     /gene="hycA"
                     /locus_tag="IT904_000457"
     CDS             36435..36896
                     /gene="hycA"
                     /locus_tag="IT904_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAP3190292.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDSEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            37021..37632
                     /locus_tag="IT904_000458"
     CDS             37021..37632
                     /locus_tag="IT904_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP3190293.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            37629..39455
                     /gene="hycC"
                     /locus_tag="IT904_000459"
     CDS             37629..39455
                     /gene="hycC"
                     /locus_tag="IT904_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAP3190294.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            39458..40381
                     /gene="hycD"
                     /locus_tag="IT904_000460"
     CDS             39458..40381
                     /gene="hycD"
                     /locus_tag="IT904_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAP3190295.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            40399..42108
                     /locus_tag="IT904_000461"
     CDS             40399..42108
                     /locus_tag="IT904_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAP3190296.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            42118..42660
                     /gene="hycF"
                     /locus_tag="IT904_000462"
     CDS             42118..42660
                     /gene="hycF"
                     /locus_tag="IT904_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAP3190297.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            42660..43427
                     /gene="hycG"
                     /locus_tag="IT904_000463"
     CDS             42660..43427
                     /gene="hycG"
                     /locus_tag="IT904_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAP3190298.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            43424..43834
                     /locus_tag="IT904_000464"
     CDS             43424..43834
                     /locus_tag="IT904_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAP3190299.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            43827..44297
                     /gene="hycI"
                     /locus_tag="IT904_000465"
     CDS             43827..44297
                     /gene="hycI"
                     /locus_tag="IT904_000465"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAP3190300.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
BASE COUNT        10410 a        11324 c        12067 g        10572 t
ORIGIN      
        1 taaaaccccg cgtactgaat tgtgatggtt tggtctcctg cacccttttt taatttatat
       61 tgtttatgtg aatattatgc ctatgtttat tattcatata tattacggat gaaaattatt
      121 tgattattca taatgtaaat attttattga atgatatata tcacagataa tttttaatat
      181 atgcgtaaat atcctttaag tttactgaag gataaaaata ttgtgacttt ttttgatttc
      241 tggggaaaaa cccgacgcgg cgagaaagac gggggcgacg actatcacct tctttgctgg
      301 cattcgctgg atgtagccgc gatgggctat ttaatggtta aaagaaattg cttcgggctg
      361 gctgattatt ttcgtcaatt agggatttct gacaaggaac aggcggctca atttttcgcc
      421 tggttgctgt gctggcacga tattggaaaa tttgcccgct cttttcaaca actttacctg
      481 ccccctgaac tcaagattca ggaaggtgcc agaaaaaatt acgaaaaaat ctctcattca
      541 acgctgggct actggttgtg gaatcattat ttaagtgaat gtcaggagtt acttccttca
      601 tcttcgctct ctccgcgcaa acttaggcga gtgatagaaa tgtggatgcc cgtgacgaca
      661 gggcatcatg gtcggccccc tgatcgtatg gatgaattgg ataattttct gcctgaagac
      721 aaagctgcag cacgagattt tctccttgaa atcaaaccac tgtttccgct catagagatc
      781 cccgcattct gggatgatga tgagggcatt gaacttataa aacacctttc ctggtatatc
      841 tctgcaaccg tcgtgctcgc agactggacg ggttcgtcaa cgcgattttt tccacgcgtc
      901 gcacacccaa tggatattaa aggttactgg cagaaaactt taattcaggc tcaaaacgcc
      961 ttaaccgtct ttcctctaaa agcaaaagtc gcgcctttca acggaattaa tacgctgttt
     1021 ccttttattg agaatccgac accgttacag caaaaggtgc tggatctgga tatcagccag
     1081 caaggaccgc agttatttat tctggaagat gtgactggcg caggtaaaac agaagcggcg
     1141 cttatcctgg cgcacaggtt gatagctgcg gggaaagctc aggggctgtt tttgggtctg
     1201 ccaacaatgg caacggccaa tgccatgtac gatcggctgg tcaaaacctg gcttgctttc
     1261 tattcgccag aatcccgccc cagcctggtg ctggcacata gtgcccgcac attaatggac
     1321 cgctttaatg aatcactctg gtctggtgat ttagtcgggt cagaagaacc ggatgaacaa
     1381 acgttcagtc agggatgtgc ggcctggttt gccgacagta acaagaaggc gttgctggct
     1441 gaaattggcg tcggcacgct ggatcaggcg atgatggcgg tgatgccgtt caaacataat
     1501 aatctgcggc ttctggggct gagtaacaaa atcctgctgg ctgatgagat ccacgcctgt
     1561 gacgcttaca tgtcgtgcat tcttgaaggg ttgatcgagc ggcaggcgcg tggcggaaac
     1621 agcgttattt tgctttctgc cacgttatct caacagcagc gcgacaaact cgtcgctgcc
     1681 tttgcgcggg gtacagaggg ccagcaagaa gctccgttcc tggaaaagga tgattacccc
     1741 tggctgacgc atgtcacgaa atccgatgtg aactcacacc gggtagcgac gcgcaaagac
     1801 gttgagcgta gcgtcagcgt gggttggctt catagtgaac aagagtgtat tgcgcgtatc
     1861 gaatcggcgg taagtcaggg gaaatgcatc gcctggatcc ggaattctgt cgatgacgct
     1921 attaaggttc atcgtcagct gcttgcccgc ggcgtcattc ctgcttccag cctttcactt
     1981 tttcatagcc gctttgcttt tagcgatcgc cagcgaattg aaacggaaac gctggcacgc
     2041 tttggtaaat attgtagttt acagagagcg tcgcaagtca ttgtttgtac acaagtaatt
     2101 gagcaaagcg tggatattga cctggacgaa atgatctccg acctggcccc tgttgatttg
     2161 ctgattcagc gagcaggacg attacagcgg catatccgcg atattaatgg tcagttaaag
     2221 cgtgacggaa aggacgagcg ttccccccct gaattgctga ttctggctcc cgtctgggac
     2281 gactctcctg gcgacgaatg gtttggcagt gccatgcgta acagtgcatt tgtctatccc
     2341 gatcatgggc gaatctggct gacgcagcgt gtgctgcgtg agcaaggtgc tattcagatg
     2401 ccacacgcag cccggcttct tattgaatcc gtctacggtg aggacgtggt aatgccggaa
     2461 ggatttgccc gcagcgagca ggagcaagtg ggcaaatatt actgtgatcg cgcaatggct
     2521 aaaaagtttg tgctgaactt caggcccggc tatgccgcca atatcaacga ttacctcccg
     2581 gaaaaactgt cgacacgtct ggctgaggag tctgtttccc tgtggctggc tacctgtatt
     2641 gacggtgtgg tgaagcctta tgccaccggt gctcacgcat gggaaatgag cgttgtcaga
     2701 gtgcgccgaa gctggtggaa aaaacatcgg gatgagtttt ctttactgga gggagaggcg
     2761 ttcaggctgt ggtgcattga acagcgacaa gatccggaga tggcaaacgt gattttagtc
     2821 aacgatgacg aaagttgcgg gtattcggct acggaaggat tgattggcaa ggttggttga
     2881 gatgtctgcg acaggactct ctgaatagtt gatacaccga aaaggatgga aagatggaca
     2941 accgacctgg gatggtcttt tttacgagag gatgcgtatg aactcgtttt cacttctgac
     3001 aaccccgtgg ttgcccgttc gttttaaaga cggaacgaca ggcaaactgg cgccagtcga
     3061 tctggcggat gaaaatgttg tcgatatttc tgcgccgcgg gcagatctcc agggtgcggt
     3121 atggcagttt ttgctggggc tactacaaac cagcttcgcg ccaaaagatc atcgtcgttg
     3181 ggatgatatc tgggaagacg gactggaagc tgaaaagcta cgggaagcat tgcagtcatt
     3241 agaacatgct ttccagtttg ggccagattc cccttcattt atgcaggatt tcgaggcgct
     3301 caccggagat aaagttccgg tcgcttcgct actgcctgag attcccggcg ctcaaacaac
     3361 gaagtttaat aaagaccact ttatcaagcg tggtgtaacg gaatacctat gcccacattg
     3421 ttctgcgtta gctctgttct ccctacagtt gaatgcgccg tcaggtggca aaggctaccg
     3481 caccggttta cgcggcggtg ggccgatgac cactctgatt gaattacagg aatatcaggg
     3541 taatcaacaa actcccttgt ggcgcaaact gtggatcaat gtcatgccgc aggatgaagc
     3601 cgatttaccg ctacccaaaa aatttgacga tctggttttc ccctggcttg gcccgacgcg
     3661 caccagcgaa ctggccggtg cggtggtaac cgatgagcag gtcaataaac tccaggcgta
     3721 ctggggaatg cctcgccgta ttcgtattga ttttaatacc acgacagtcg gcaactgcga
     3781 tatttgcggt gagcagagcg acgcgctcct gagcctgatg acgaccaaaa attacggtgc
     3841 gaattatgcc atgtggcagc atcccttaac gccttaccgt gtaccactta aagagggtgg
     3901 cgagttttac tccgttaaac cacaaccggg cggtttaatc tggcgtgact ggttaggcct
     3961 tatcgaaacg ggtaaatcag aaaacaatac ggaacttccc gcgctggtgg tgaaactctt
     4021 caatgccagc agtctcaagc aggcaaaagt gggcctgtgg ggatttggct atgatttcga
     4081 caacatgaaa gcgcgctgtt ggtacgaaca ccatttcccg ctgctgctcg ataaaaaaga
     4141 aggtcagata ccgaagctgc ggctggctgc gcaaacagct tcacggattc taagtctgtt
     4201 acggagtgca ttgaaagaag catggttctc cgatccaaaa ggtgcaaggg gtgattttag
     4261 ttttgtggat atcgacttct ggaacaaaac tcagcatcgc tttctgaggt tagtgcgcca
     4321 aattgaagaa ggtcaggatg cggatgaatt actcagcaaa tggaataagg aaatttggtt
     4381 attcgcacgt caggattttg acgagcgtgt attcaccaat ccttatgagc ccgttgatct
     4441 ggaacgcgtc atgaccgcgc gcaagaaata ttttacaaca tcggcggaga agcaaagtgc
     4501 taaagccgcc agggagaaaa agcaggaggc tgctgaatga gtattgtaaa agaggagcac
     4561 aaagccacgc tgcgcaaatg gcacgaggaa ttgcaggaga aacgtggcga acgagccagt
     4621 ctgcggcgta gtacgacggt taatgatgtt tgtctgacgg atggatttcg cctgtttctg
     4681 aagaacagac aaataaagtg gcaggatgaa cctgagtggc gtatcaccgc actggcgctg
     4741 atagccgcag ttagcgccaa tgttaaagct attgatgaac ggcagccttt tgccgcgcag
     4801 ctggcggcgg taatgtcaaa ggggcgtttt actcgtctgt cggcagtaaa aacaccggat
     4861 gaattactgc gccagttacg ccgcgcagtc agattattaa acggttccgt caatcttgac
     4921 tctctggcag agggggtctt ccgttggtgc caggagagcg acgacctgct caatcatcac
     4981 cgccgccagc agcggccaac cgaatttatc cgtatccgct gggcgctgga atattaccag
     5041 gctggcgacg ccgataacga acaaaatcaa taaaggacta tctcatgaca acatttattc
     5101 agcttcattt gttaaccgct taccctgctg ccaaccttaa ccgtgatgat accggtgcgc
     5161 caaaaactgt ggtgctgggt ggagcaacgc gtctgcgcgt ttcctcgcaa agtctgaaac
     5221 gtgcgtggcg cacttctgca ctttttgaac aggcactggc gggccatatt ggtattcgca
     5281 gtgggcgtat tgcgcgtgag gcggcaacta tcctgattga gaaaggaatc gaagagaaaa
     5341 aagccatcga atgggcggca aaaattgcag attatcttgg gaaagctaaa aacgacaaaa
     5401 aaccaaaaga tccgctcact aacgccgaaa ctgaacagtt agtccatatc agcccggcag
     5461 aatttgacgc cgtaaaagtg ctggcccatc agcttgcaga agaaaagcgc gcgccaaaag
     5521 aggaagatct cgcgttgtta cgcaaagacc gtatggcggt agatattgcc atgtttggtc
     5581 gtatgctggc gaataaaccc gagtttaatg ttgaagccgc ctgccaggta gcgcacgcat
     5641 ttggtgtcag tgaaacgatt gtcgaagatg attttttcac cgccgttgat gatttgcgcc
     5701 aggcttctga agatgccggt gccgggcatc ttggtgaaac cggattcggt tctgcactgt
     5761 tctacaccta tatctgcatt gataaagatc tgctggtcga aaaccttggc ggtgacgaag
     5821 cgttagctaa tcagaccata cgtgccttta cggaagccgc acttaaagtc tccccaaccg
     5881 gcaaacagaa cagctttgcc agccgtgcct acgcctcctg ggcgctggca gaaaaaggca
     5941 ccgatcaacc gcgttctctg gcggcagctt tctatgaacc cattaatggc acccgtcagt
     6001 tagaggtggc ggtgcagcgt attacaactc ttcgcgaaaa tatgaatacg gtctacgaac
     6061 agaagaccga ttacgcaagt tttgacgtga tgaacaaaca gggaagcatg aaggacgtgc
     6121 tggactttat ctgcgcgtaa ggggcggctt atgagccaat atctgatttt tcagcttcat
     6181 gggccgatgg cgtcctgggg tgtcgatgct ccaggtgaag ttcgtcatac ccatgaactt
     6241 ccttcgcgct cagcattgct ggggctactg gctgccgggg gagggattcg gcgtgatgat
     6301 accgaacgct taaacgcgtt taatcgccac tattcactgg tggtttgcgc cagccgtaac
     6361 ccgcgctggg cacgggatta tcacacggtc cagatgccaa aagaggtgcg taaagcacgt
     6421 tatttcagcc gtcgcgaaga gttgagcgac cctgagcttt tgagcgcgat tatctcccgg
     6481 cgcgactact acaccgatgc ctggtggatg gtggcggtgg caacaacccc cgatgcgcct
     6541 tacagcctcg aacagttgca ggacggttta cgccatccgg tttttccgct ttatctgggg
     6601 cgaaaaagtc atccgctggc gttaccactt gcgccgttac tgctcgaagg caacgcgtct
     6661 gatgtcttac gtaacgcata ccaacagtat caggatcgtt tccgcgaact gaaagtctca
     6721 ctcccgaaac ttcaggatga atgctggtgg gaaggggagc acgatggttt ggtcgcaagc
     6781 aaaatattac gccgccggga tgttccttta aatcgtcagc agtggctgtt tggggaacgc
     6841 accatcaatc aggggccgtg gctcagcaag gaggaaccat gtacctctca agaataacct
     6901 tacataccgg ccagctttcg cctgcgcagt tgctgcatct ggtggatcgc ggtgaatatg
     6961 tgatgcatca gtggttgtgg gatctcttcc ctggcggcaa agaaaggcaa tttctttatc
     7021 gtcgggaaga actccagggc gcgtttcgct tttttgtcct ctcgcaggaa cgcccggcag
     7081 aaagtgaaac tttcaccatc gaatgccggt catttacgcc cgagctgagt accggacaac
     7141 aactttgttt caacctgcgg gcgaatccaa caatctgtaa agcgggcaag cgccacgatc
     7201 tgctgatgga ggcgaaacgg caggtgaggg gacaggcgga aggaagtgat gtctggttgc
     7261 atcaacaaca ggcggcgctg gactggctgg cagcgcaggg ggaaagaagt ggttttacac
     7321 tactggatac ctcggttgat gcctaccgac aacaacaact ccggcgggaa aactcctggc
     7381 aactgatcca gttcagtagt gtggattata cggggatgct tacggtcacc gatcctgggt
     7441 tatttctgca acgtctcagt caggggtatg gcaaaagccg ggcgttcggt tgtggtctga
     7501 tgctgatcaa acctggagca gaggcgtgac gtttgtaccg ctgagcccga tcccgttaaa
     7561 agatcgcacc tccatgatct tcctccagta cggtcaaatc gacgtactgg acggcgcttt
     7621 cgtgctgatc gacaaaaccg ggatccgcac gcacattccg gtgggatcgg tcgcctgcat
     7681 tatgctcgaa ccgggaacga gagtttccca cgcggcggtg catctggccg ccacggtggg
     7741 gacgctactg gtctgggtcg gtgaagcggg tgttcgcgtc tactcttccg gacaacccgg
     7801 tggggcgcgt gctgataagt tactctacca ggcaaagctg gctttaacgg aagatctacg
     7861 cctgaaggtg gtgcgcaaaa tgtatgaatt acgttttcgt gagccaccgc cagctcgccg
     7921 ttcagtggag cagttacggg gaattgaggg atcccgcgtt cgccagacgt atgcattact
     7981 ggcgaaacaa tatggtgtga aatggaatgg tcgcaaatac gatcctaaag actgggaaaa
     8041 aggcgatgtt gtcaatcgtt gcatcagtgc tgccacatca tgcctgtacg gtatttctga
     8101 agctgcagta ttagctgcgg gatatgcgcc cgctattggt tttatccata gcggcaaacc
     8161 gctttcattt gtttatgaca tagccgatat cattaaattt gatttggttg tgccaaaggc
     8221 atttgaaata gcagcgaggc aacccgcaga accagataaa gaagtcaggt tggcctgtcg
     8281 cgatattttc cgtagtagta aattaacggg caagttaatt ccgttaattg aggaagtgct
     8341 tgccgctggt gaaattgaac caccacaacc cgcgccggat atgttaccgc ctgccatccc
     8401 tgaacctgaa accttaggcg atagcggtca tcgaggacgt ggcggatgag tatgatcgta
     8461 gttgtaacgg aaaatgttcc tccacgttta cgtggacggc tcgctatctg gctactggaa
     8521 gtgcgtgccg gtgtgtatgt cggagataca tccaaacgta ttcgggagat gatctggcag
     8581 caaatctctc aactggcagg ttgcggaaat gtagtgatgg cttgggcgac caataccgag
     8641 tcaggttttg aatttcagac ctggggtgaa aatagacgta ttccggtgga tttggatggg
     8701 ttgcgtttgg tttcttttct tcctgttgat aatcaataag ttatccgttc tttaaaaata
     8761 aggaaatgtt ttaatttagt tggtagattg ttgatgcgga ataaatttgt ttaaaaacag
     8821 ttatgtatgc ttagtgtgtt ccccgcgcca gcggggataa accggagcgt agagattctc
     8881 gaaaccgatc cggacggtgt tccccgcgcc agcggggata aaccgaataa ataataatcc
     8941 agtagcccca tgtattagtg ttccccgcgc cagcggggat aaaccgcgat aaccgcgtca
     9001 gtattggaca ggatatcagt gttccccgcg ccagcgggga taaaccgaat ctattgtgaa
     9061 tttgaaatgg tccagcactg tgttccccgc gccagcgggg ataaaccggg taaaaacacg
     9121 gtctgaaccg acattcatgt gtgttccccg cgtcagcggg gataaaccgg gcgcactgga
     9181 tgcgatgatg gatatcactt ggagttcccc gcccctgcgg tagaactccc aactcccatt
     9241 ttcataccca tcaagacgcc ttcgccagct ccttcaccag cggtagcatt atccgcataa
     9301 catcacggca gcgacgttct attcttcctg gaagtgcctt atcaatatgt tgttgattat
     9361 ccagtcttac gtcatgccag ctatttcccg cagggaatgc agctgttttt gcgcgttgct
     9421 gataaccatc cttattccca agattccaat ttgttgcttc taccgaaagt acggcaatac
     9481 cggctttgtc gaaaacttcg gcgtcattac aacagccagt acccttcgga taatttttat
     9541 tcaaacccgg attggtcgtt gcggcaattc catgactacg cgcaattgcc agtgccctgt
     9601 cgcgcgttaa tttccttact gcttcagggg tttttacacc actgttgaaa tacaatttat
     9661 cgccaacaat taagttatcg agattaatca ccagcagcgt atttttcttt tcggtgtcac
     9721 tcatccgctt gagtaaattc tcagcgccta atttcccttc ctcttcaccg ctggtcgcta
     9781 caaatcgaat accatactcg gtaggcgtat ttttcaggcg ttctgccaat tccagcatga
     9841 cacctaaacc tgcggcgtta tcatccattc cttgtaacgt cagcccgccg agattggcat
     9901 cggcgtcagc atcgctcagc ggggcgtaag tatccagatg cgccataatg atgatctgct
     9961 gcggcgcttt gccttcatga gcggcaatca ccgtacttcc cgtcacgtta tgccagctct
    10021 tacgattatc gcgggcggta taaatatacc gactattaaa tgtccgaata tcactgcgat
    10081 aacccatttg ctgaaactgt tggcgaatat aatcggcaga taacatttct gcaggagttc
    10141 cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc ctgagtatta gcaaaatcgc
    10201 caggtttagg tgacgaggcg tgtacgggga gaataaagca tacgccgagc gccagggcag
    10261 cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta aataccatgc aaattttttt
    10321 accgccatag tatgaaactg ccgctgcgct aaaacaattt caaatcttcc taaacgcccg
    10381 aaatccggtg ccttaagcac tttttgatat tagctttgcc aaatcgttat tccgttaagg
    10441 aactactcat tctaattggt aatttcattc gttctcttac gctccctata gtcgaaacat
    10501 ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta tggatcaaat acgacttact
    10561 cacctgcggc aactggaggc ggaaagcatc cacattattc gcgaggtggc ggcagaattc
    10621 tcaaatccgg tgatgctcta ctctatcggt aaagattcca gcgtcatgct gcatctggcg
    10681 cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc tgcatgtcga taccggctgg
    10741 aaattccgcg agatgtatga gttccgcgat cgtacggcga aagcctacgg ctgcgagctg
    10801 ctggtgcata aaaacccgga aggcgtggcg atggggatta atccattcgt gcacggcagc
    10861 gcgaaacata ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa caaatacggt
    10921 tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc taaagagcga
    10981 atctactctt tccgtgaccg cttccatcgc tgggatccga aaaatcagcg cccagagctg
    11041 tggcacaact acaacgggca aattaacaaa ggcgaaagca tccgcgtctt cccgctctct
    11101 aactggaccg agcaggatat ctggcaatac atctggctgg aaaatatcga cattgtgccg
    11161 ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg gtatgttgat gatgattgat
    11221 gacaaccgta tcgacctgca accgggcgaa gtgattaaaa aacggatggt gcgtttccgt
    11281 acgctgggct gctggccgct gaccggtgcg gtggagtcaa atgcacaaac actaccggag
    11341 atcatcgaag agatgctggt ttctaccacc agtgaacgtc agggccgcgt gattgaccgc
    11401 gaccaggcgg ggtctatgga gctgaaaaaa cgtcaggggt atttttaaga tgaacaccgc
    11461 acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg cgcaacaaca
    11521 taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa gtaccctgat
    11581 tggtcgcctg ctgcacgata cccgccaaat ctacgaagat cagctctcat cgctgcataa
    11641 cgacagtaaa cgtcacggca cccagggcga aaagctggat ctggcactgc tggtggacgg
    11701 cctgcaagct gagcgcgaac agggcatcac cattgacgtg gcttaccgct atttctctac
    11761 cgagaagcgt aaatttatta tcgccgacac cccagggcac gagcagtaca cccgcaatat
    11821 ggcgactggc gcatcgacat gtgaactggc gatcttactg atcgatgccc gtaaaggcgt
    11881 gctcgatcaa acccgtcgtc acagttttat ctccacactg ttggggatca aacatctggt
    11941 cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa aagacgttca cccgtattcg
    12001 tgaagattat ctgacctttg ccgggcagct gccgggtaat ctggatatcc gctttgtgcc
    12061 gctctctgca ctggaaggcg acaacgtggc ttcgcaaagt gaaagtatgg cgtggtacag
    12121 cggtccaaca ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg tggatgctca
    12181 gccaatgcgc ttcccggtgc agtacgttaa tcgcccgaat ctcgattttc gtggttacgc
    12241 cggaacgctg gcatccggtc gcgtagaagt cgggcaacga gtcaaagtgc tgccctctgg
    12301 tgtggaatca aacgtcgccc ggatcgtgac ttttgatggc gatcgcgaag aagcctttgc
    12361 cggagaagcg atcaccctgg tgctgacgga tgagatcgac atcagccgtg gcgatctgct
    12421 gctggcggca gacgaagcgt taccagctgt gcagagcgcg tcggtggatg tggtatggat
    12481 ggcggaacag ccgctttccc cgggccagag ttatgacatc aaaattgccg gtaagaagac
    12541 gcgtgctcgt gttgatggca ttcgttatca ggttgatatt aataacctta cccaacgcga
    12601 agttgaaaac ctgccgctga acggcatcgg cctggtggat ctcacttttg acgagccact
    12661 ggtgttagat cgttatcagc aaaacccggt taccggtggg ctgattttta tcgatcgcct
    12721 gagcaatgtg accgtaggtg ccggtatggt gcatgagcca gttagccagg caactgctgc
    12781 gccatctgaa tttagtgcat tcgaactgga attgaatgcc ctggttcgcc gccactttcc
    12841 acactggggc gcgcgcgatt tgctggggga taaataatgg cgctgcatga cgaaaacgtc
    12901 gtctggcata gccatccggt cactgtgcaa caacgcgagc tacaccacgg tcatcgtggt
    12961 gtagtgctgt ggtttaccgg cctctccggg tccggtaaat caacggtcgc cggggcgctg
    13021 gaggaggcgt tacataaact cggcgtcagt acgtatctgc tggatggcga taatgttcgc
    13081 cacggattat gcagcgatct cggttttagc gatgccgatc gtaaagagaa tatccgtcgc
    13141 gtcggtgaag tggcgaattt gatggttgaa gccggactgg tggtgctgac cgcatttatc
    13201 tcgccacacc gcgccgaacg ccagatggtt cgtgaacgcg taggagaagg gcgctttatc
    13261 gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa aggcttatat
    13321 aagaaagcgc gtgccggtga actgcgctac tttacgggaa tagattccgt ttacgaagcg
    13381 cctgaatcgg cagaaattca tctcaatggt gaacaattag taacaaattt ggtacagcaa
    13441 ttattagatc tgttgagaca gaacgatatt atcagatcct gagacaccac cgggtttcca
    13501 tgcccggttc gtcaaagtaa caggatcaaa tatgcgtaat agccataaca ttacactaac
    13561 aaataacgac agccttactg aggatgaaga aaccacatgg tcactgcctg gtgccgtggt
    13621 cggttttatc tcctggttat ttgcgctggc gatgccgatg ttgatttatg gctctaacac
    13681 gctgttcttc tttatctaca cctggccttt ctttcttgcg ctgatgcccg tcgcggtagt
    13741 ggtggggatt gcgctgcatt cattgatgga cggaaagcta cgctacagta ttgttttcac
    13801 cctggtgaca gtaggcatta tgtttggcgc actgtttatg tggctactgg gctaatttgt
    13861 actttcccgt ctcctctgtt cataattcaa accgtaacta ataatgagat tatgttctgc
    13921 acgccctggg tatacgtaac aatggacaaa tgtggtacat ttgcccgcgt tgtcgcggta
    13981 tccccaacag aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc cgtttttcag
    14041 ggggcaggat gggtaaacta acgctgctgt tgctggctat tctggtctgg ctacagtatt
    14101 cgctgtggtt cggtaagaac ggtatacatg actatacccg cgtcaatgat gatgtggcgg
    14161 cacagcaagc tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt gccgaaattg
    14221 acgatctcaa tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa ctcagcatga
    14281 ccaggccggg cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc gcacagtctg
    14341 cggggcaaaa caatcgataa atcagcccgg gaattaacat ggcaaccact catttggatg
    14401 tttgcgccgt ggttccggcg gccggatttg gccgtcgaat gcaaacggaa tgtcctaagc
    14461 aatatctctc aatcggtaat caaaccattc ttgaacactc ggtgcatgcg ctgctggcgc
    14521 atccccgggt gaaacgagtc gtcattgcca taagtcctgg cgatagccgt tttgcacaac
    14581 ttcctctggc gaatcatccg caaatcaccg ttgtagatgg cggtgatgag cgtgccgatt
    14641 ccgtgctggc aggtctgaaa gccgctggcg acgcgcagtg ggtattggtg catgacgccg
    14701 ctcgtccttg tttgcatcag gatgacctcg cgcgattgtt ggcgttgagc gaaaccagcc
    14761 gcacgggggg gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc gaaccgggca
    14821 aaaatgccat tgctcatacc gttgatcgca acggcttatg gcacgcgctg acgccgcaat
    14881 ttttccctcg tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa ggcgcgacta
    14941 ttaccgacga agcctcggcg ctggaatatt gcggattcca tcctcagttg gtcgaaggcc
    15001 gtgcggataa cattaaagtc acgcgcccgg aagatttggc actggccgag ttttacctca
    15061 cccgaaccat ccatcaggag aatacataat gcgaattgga cacggttttg acgtacatgc
    15121 ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc attccttacg aaaaaggatt
    15181 gctggcgcat tctgatggcg acgtggcgct ccatgcgttg accgatgcat tgcttggcgc
    15241 ggcggcgctg ggggatatcg gcaagctgtt cccggatacc gatccggcat ttaaaggtgc
    15301 cgatagccgc gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg gttataccct
    15361 tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag atgttgccgc acattccaca
    15421 aatgcgcgtg tttattgccg aagatctcgg ctgccatatg gatgatgtta acgtgaaagc
    15481 cactactacg gaaaaactgg gatttaccgg acgtggggaa gggattgcct gtgaagcggt
    15541 ggcgctactc attaaggcaa caaaatgatt gagtttgata atctcactta cctccacggt
    15601 aaaccgcaag gcaccgggct gctgaaagcc aatccggaag actttgtggt ggtggaagat
    15661 ttgggctttg agcctgatgg tgaaggtgag catattctgg ttagaatcct caaaaacggc
    15721 tgcaataccc gttttgtggc ggatgcactg gcgaaattcc tgaaaattca tgcccgtgaa
    15781 gtcagcttcg ctgggcaaaa agacaaacat gctgttacgg aacagtggtt atgcgctcgc
    15841 gtgccgggca aggaaatgcc cgatctgagc gcctttcaac tggaaggctg ccaggtgctg
    15901 gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc tgaaaggaaa cgcctttacc
    15961 ctggttctgc gcgaagtgag caatcgcgat gacgttgaac aacgtctgaa cgatatttgc
    16021 gtaaaaggtg taccgaacta cttcggtgcc caacgttttg ggattggcgg tagcaacttg
    16081 cagggggcgc agcgctgggc gcaaaccaat actccggtgc gcgatcgcaa taaacggagt
    16141 ttttggttgt cggcagcccg cagtgcgttg tttaatcaga ttgttgctga gcgcctcaaa
    16201 aaagcagacg ttaatcaagt tgttgacggc gatgcgctac aattagccgg acgtggtagc
    16261 tggtttgtcg caaccaccga agaactggcg gaattacagc gtcgcgttaa cgataaagag
    16321 ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg gaactcagcg tgaagcgctg
    16381 gcattcgaac aagcagctgt cgccgcagaa actgaattac aagctttact ggtgcgcgaa
    16441 aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag ctggaattgg
    16501 tgggatgacg tcaccgtaga gatccgtttc tggcttccgg cgggtagttt tgcaaccagc
    16561 gttgtcaggg aacttatcaa cacaacaggt gattatgcgc atattgctga gtaatgatga
    16621 cggggtacat gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt ttgctgacgt
    16681 tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca aattctctga cactggaatc
    16741 ctccctgcgc acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg gaaccccgac
    16801 cgattgcgtc tatcttggcg tgaatgctct gatgcgtccg cgcccggaca ttgttgtgtc
    16861 cggaattaac gccgggccga atctggggga tgatgttatt tattccggta cggtagccgc
    16921 cgcgatggaa ggccgtcatt taggttttcc ggcgcttgcc gtctcgcttg acgggcataa
    16981 acattacgac actgccgcgg cggtaacctg ttcaattttg cgcgcactgt gtaaagagcc
    17041 gctgcgcacc gggcgtattc ttaatattaa cgttccggat ttacccttgg atcaaatcaa
    17101 aggtattcgc gtgacgcgct gcggtacacg acatccggca gatcaggtga tcccgcagca
    17161 agatccgcgc ggcaatacgc tgtactggat tggcccgccg ggcggtaaat gtgatgccgg
    17221 tccggggacc gattttgctg cggtagatga gggctatgtc tccatcacgc cgctgcatgt
    17281 ggatttaact gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca gcgtgggagt
    17341 tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg atcaattacg tgcgcaaggt
    17401 attcaggatg agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa attcgttgat
    17461 gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc cgataggtca ggggcagaca
    17521 atttcgcagc catatatggt ggcgcgaatg accgaattac tcgagctgac gccgcagtcg
    17581 cgggtgctgg aaattggcac cggttcggga tatcaaacgg caatcctggc gcatcttgtc
    17641 cagcatgttt gctcggttga acggattaaa ggcttgcagt ggcaggcacg tcgccgcctg
    17701 aaaaatcttg atttacataa tgtttcaacc cgtcatggcg atggatggca aggttggcag
    17761 gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac cgccggaaat tccaactgcg
    17821 ctaatgacgc agctggacga aggcgggatt ctcgtcttac ccgtagggga ggagcaccag
    17881 tatttgaaac gggtgcgtcg tcggggaggc gaatttatta tcgataccgt ggaggccgtg
    17941 cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata cctggatttt
    18001 tcctggttat tttgccgcag gtcagcgtat cgtgaacatc ttttccagtg ttcagtaggg
    18061 tgccttgcac ggtaattatg tcactggtta ttaaccaatt tttcctgggg gataaatgag
    18121 cgcgggaagc ccaaaattca ccgttcgccg cattgcggct ttgtcactgg tttcgctatg
    18181 gctggcaggc tgttctgaca cttcaaatcc accggcaccg gtcagctccg ttaatggcaa
    18241 tgcgcctgca aatactaatt ctggtatgtt gattacgccg ccgccgaaaa tggggacgac
    18301 gtctacagcg cagcaaccgc aaattcagcc ggtacagcag ccacaaattc aggctactca
    18361 acaaccgcaa atccagccag tgcagccagt agctcagcag ccggtacaga tggaaaacgg
    18421 acgcatcgtc tataaccgtc agtatgggaa cattccgaaa ggcagttata gcggcagtac
    18481 ctataccgtg aaaaaaggcg acacactttt ctatatcgcc tggattactg gcaacgattt
    18541 ccgtgacctt gctcagcgca acaatattca ggcaccatac gcgctgaacg ttggtcagac
    18601 cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca ttacccaggc
    18661 cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg ttgctgttgc
    18721 gtcgcaaccg acaattacgt attctgaatc ttcgggtgaa cagagtgcta acaaaatgtt
    18781 gccgaacaac aagccagctg cgaccacggt cacagcgcct gtaacggtac caacagcaag
    18841 cacaaccgag ccaactgtca gcagtacatc aaccagtacg cctatctcca cctggcgctg
    18901 gccgactgag ggcaaagtga tcgaaacctt tggcgcttct gaggggggca acaaggggat
    18961 tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc gcgttgttta
    19021 tgctggtaac gcgctgcgcg gctacggtaa tctgattatc atcaaacata atgatgatta
    19081 cctgagtgcc tacgcccata acgacacaat gctggtccgg gaacaacaag aagtgaaggc
    19141 ggggcaaaaa atagcaacca tgggtagcac cggaaccagt tcaacacgct tgcattttga
    19201 aattcgttac aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc gataaatcgg
    19261 cggaaccagg cttttgcttg aatgttccgt caagggatca cgggtaggag ccaccttatg
    19321 agtcagaata cgctgaaagt tcatgattta aatgaagatg cggaatttga tgagaacgga
    19381 gttgaggttt ttgacgaaaa ggccttagta gaagaggaac ccagtgataa cgatttggcc
    19441 gaagaggaac tgttatcgca gggagccaca cagcgtgtgt tggacgcgac tcagctttac
    19501 cttggtgaga ttggttattc accactgtta acggccgaag aagaagttta ttttgcgcgt
    19561 cgcgcactgc gtggagatgt cgcctctcgc cgccggatga tcgagagtaa cttgcgtctg
    19621 gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga ccttatcgaa
    19681 gagggcaacc tggggctgat ccgcgcggta gagaagtttg acccggaacg tggtttccgc
    19741 ttctcaacat acgcaacctg gtggattcgc cagacgattg aacgggcgat tatgaaccaa
    19801 acccgtacta ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta cctgcgaacc
    19861 gcacgtgagt tgtcccataa gctggaccat gaaccaagtg cggaagagat cgcagagcaa
    19921 ctggataagc cagttgatga cgtcagccgt atgcttcgtc ttaacgagcg cattacctcg
    19981 gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc tggacatcct ggccgatgaa
    20041 aaagagaacg gtccggaaga taccacgcaa gatgacgata tgaagcagag catcgtcaaa
    20101 tggctgttcg agctgaacgc caaacagcgt gaagtgctgg cacgtcgatt cggtttgctg
    20161 gggtacgaag cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac ccgtgaacgt
    20221 gttcgccaga ttcaggttga aggcctgcgc cgtttgcgtg aaatcctgca aacgcagggg
    20281 ctgaatatcg aagcgctgtt ccgcgagtaa gtaagcatct gtcagaaagg ccagtctcaa
    20341 gcgaggctgg ccttttctgt gcacaataaa aggtccgatg cccatcggac ctttttatta
    20401 aggtcaaatt accgcccata cgcaccaggt aattaagaat ccggtaaaac cgagaatggt
    20461 cgttaacact gtccaggttt tcagaccgtc tgctaccgac aaccccagat atttggtcac
    20521 aatccagaac cctgagtcat taatatgtga cgcgccaagc ccaccaaagc aggctgccag
    20581 cgtcaccaat acgcactgaa tcggattcaa tcccatcacc gcttctgaga gtaacccgcc
    20641 ggttgtcagg attgctacgg ttgctgaccc ctgcgatgca cgcagcgcca gtgaaataat
    20701 aaatgcggct ggtaacagag gcaggtcaat catttgtagc atgttggcaa gggctttgcc
    20761 gacgcccgat tccaccagca ctttgccaaa tacccctcca gcaccagtaa ccaaaatcac
    20821 taccgccgca gtgggaaggg ctgagcccat aatgtcgctg gtgtgttgta agctccagcc
    20881 gcgacgtaaa gccaataacc agaatgccag caccagcgca atcattagag ctaccattgg
    20941 tgagccgagc agctgtagcg taccaagcag gggatgcgaa ggcggcatca gtgttgcgga
    21001 aaccgtaccc gccatgataa tcgcgatagg aataacaatt agcgaggtga ccagcgcgac
    21061 gcccggtgga tttattttat cgcttaattt tgtcgcgcct tcctcactgg ccggagccag
    21121 ttgcatctgt tccagtactt ctactgacat cgcatattgg cgcttattga ttattttcgc
    21181 tgcaaagtag ccaacaaccc ctacgggaat agaaatcgca ataccgatga tggttagcca
    21241 gccgatgtct gcgtggagta accccgctgc ggcgacaggg cctggatgcg gcggtaccgc
    21301 cacgtgaaca gtgagcatga tcccagcgac aggcaggcca aatttgagtg gcgatatttt
    21361 ggcaaccttg gcaaaaccgt aaatgattgg cgcaagaata ataaagccga catcaaagaa
    21421 gacgggaata ccgaggaaga acgctgccag agtcagcgca gcgatagttc gtttgtcacc
    21481 taacttgcga ctgaaataat tagccagtga ctctgcacca ccagagtgtt cgatcatacg
    21541 ccccagcata gcgcccagac caataataat agtgacggaa ccaagcacac cgcccatccc
    21601 ggcgatcatc actttaccca cttcgcccgc cggtataccc gccgcaagtg cgactaacag
    21661 gctgacgagg agcagagcaa cgaatggttg tacctttgcc ttgatgacca gcagcaacag
    21721 catgattacg ccagttaacg caatgcataa caatgtaatt gtggacatgg gaaaccctgt
    21781 ctgaaagtta tagttaacct accccatccg tagatggggg gatgtatggg tacgttgtaa
    21841 ttagggattt aacgaattag cgccaggcgt caaaccagcc aagcccttct tcggtgaggc
    21901 cacgaggttt atattcacaa ccgatccagc cctgatatcc cacctcatcg aacaggcgga
    21961 acagccacgg atagttgatt tctccatcgt ccggttcatg tcgatcaggt agtccggcaa
    22021 tttgtacgtg cgcatatttc ccggcgtagt cgcggattaa atgcgtcagg ttgccatcta
    22081 ctttttgcgc atgaaaagta tctagttgaa taaacacgtt atctcgcgca acctcttcaa
    22141 caatagccag tgcctgatac tggctggaga agagataatg aggcttaacg ccggggctga
    22201 gtgcttcaac taatattcgc ttgccgtgtg gcgcaaagcg gtcggcagcg tagcggatat
    22261 tatcgataaa tactgcccgg taccgttcag catcttcgcc agcgggcacg acgcctgcca
    22321 tcacatggac ttgttcacaa ttgagcgcca atgcatattc cagtgccagg tcgatgtctg
    22381 cgtgtgcttc gtgctcacgt ccgggaaggg cggataatcc ccattccccc gcattaatat
    22441 ctccgggagc ggtattgaac agcgccagtg tcagatggtt ttgctccagt tgcttttgga
    22501 tttgcagggt ggagtagtca tagggaaaca gaaattccac agcatcgaac ccggcttttc
    22561 gcgctgcggc gaagcgttca ataaaaggca cttcggtgaa catcatggat aaattagctg
    22621 caaaacgagg cattgcatta actccttaat tccgcaattt cacctgcggt cagataacgg
    22681 atcgggcggt caccgagaat aaaaatcagc tttgccgttt cctccagctc ttccatattg
    22741 ttggcggctt cttgcaggct ttcaccgcaa accactgggc catgatttgc cagtaaaaaa
    22801 gcctgattgt ctgctgccag ttccgccaga tcctgtgcga tgcgtttatc gcccggtcgg
    22861 taataaggca ccagcgggac atttcccatc cgcatcacca cgtatggtgt gaacggacga
    22921 ataacgttgc tgctgtccag cccttgcagg caggaaagcg ccgtcgacca tgtgctgtgc
    22981 aaatgcacca ccgctttaca acgcggattg ttgcgataca gcgccagatg aaagagcacc
    23041 tctttcgagg gtttgtcacc acttaaccat tcgccatccg cggcgacttt ggaaagccgc
    23101 tgcggatcga gattgcccag gcatgaacct gtcggtgtcg ccagtaaatt cccgtcaggt
    23161 aaaagcagcg acagattgcc agccgaaccg gttgcatagc cgcgctgaaa gaatgaactg
    23221 gcaatccgcg tcatctcctc tcgcaaagac tgctctactt ttgcgaaatc gctcatgata
    23281 aaaactctct ttgggctcgt gaaaaaaagg cgtcatcacc gaagttgcca gatttaaggg
    23341 cgagtgagac aggcttatcc agtgcgttta cccacggcac gccgggggaa atggttgggc
    23401 caatatgaaa cccttttatt cccaggctct gtgtgactac gccggaggtc tcaccgcctg
    23461 cgacaataaa gcgtgtcacg ccttccgctg ctaaccgcgc cgctagttga gaaaacagtg
    23521 tttctactgc ctgactggct ttttgtgcac cgtattgctg ttgaattgct gccaatgcgt
    23581 cagtgctggc ggtggcaaaa accagtggag caagtacact ttcctggccc agaacccact
    23641 ctgccagttc gtgtgcataa gcggccagag tttcaattga gaggcagcgt gccacatcaa
    23701 cttcacgggc tggtgcaatt tgacggtaat gtgccacctg gcggttggtc atttgagagc
    23761 atgaaccgga gagcactacg ccgcgcccag cgagcggatg ccctgcttcg cgagcctggt
    23821 taccgttttc ttgcgcccac tgccgggcca ggccaatcgc cagaccagaa ccgcccgtta
    23881 ccagtggggc atcgcgcaag gcttctccct gaatttccag atggtgttcg gtcagcgcat
    23941 caagcaccgc gtagcggtag ccctcttgct gtaagcgagc cagctcttga cgaacggcat
    24001 ccacaccttg ttcgaaaaca tgtgccgaaa cgacgccgca gcgccctgtg gattgcgctt
    24061 caaccagacg gggaagatag ctgtcggtca tgggatttac cgggtgatgg cgcatcccgg
    24121 attcggccag cagttgattc attacgaaca aatacccctg ataaaccgta cgtccgttga
    24181 ccggcagggc cggagagaag accgtaaacg gcgtgtcgag agcatccatt aatgcatcgg
    24241 taaccgggcc gatattacct ttcgccgtac tgtcgaaagt agagcagtat ttgaaataga
    24301 tctgtttgca accttgctgt tgcaaccagc tcagagccgc cagcgattgc tgtgtggctt
    24361 caaccaccgg acaggagcgc gttttcaggc tgatcaccag tgcgtcgatt gcttccggca
    24421 ttttacctgt tggaacaccg ttaatttgta ccgttggtag accgttttcc accagaaaac
    24481 tggcgatatc cgtcgcgccg gtaaaatcat cggcgataac gccaatcttg atcatgattt
    24541 cgctcccggt agagtgatgc cagagaaaat cttgataact gcgctatcgt cttctttccc
    24601 gtaacctgcg ttactggcgc tggtgaacat attcaatgct gttgaggcca atggcagcgg
    24661 gaagtgcagg gctttggctg tatcggcaac cagaccaaga tccttaacaa aaatatcgac
    24721 ggctgaatgc ggggtgtaat cgccatccac cacatgacgc atccggtttt cgaacatcca
    24781 ggaatttccg gcggcattgg tcacgacgtc atacatcaca tccagcggga tccccgcacg
    24841 ggctgcaagt gccatcgctt cggctccgga agcaatatgt acgcccgcta acaactggtg
    24901 aataattttt acggtcgaac ctagtcccgg ttctgcacct atgcgataaa cttttccggc
    24961 aacggcttcc agcacgggtg ccagtcgttc aaaggcaata tcgctaccgg aggccatgac
    25021 agtcatttca ccgttagcgg cttttactgc accaccagaa actggcgcat ccagcatttc
    25081 cagatcgaat ccagccagag cgatagcaat ttcttgcgca tcagcactag cgatagtgga
    25141 agaaaccatt actgccgtac cgggtttcag atgttgtgca acgcctgttt caccaaacag
    25201 cacctgttta acctgggccg cattgaccac cagcaccagc agagcgtcca gtttttcggc
    25261 aaacgtcgcg gcgttatcag aaaccccgca agcacctgcc tctttcaacg tagcgcaggc
    25321 attgctgttc aggtctgcgc cccaggtaga aagacctgcg cggacacatg acagtgctgc
    25381 tcccattccc attgacccta agccaacgat accgacatga aactcagatc ccgttttcat
    25441 ctgctctcct tgttaattta agtgatattt tgtttgatat tgtgaatata agcgctggaa
    25501 gataacgata tggtgagctg attcacataa attaacattg tgtgttattt tatgtgaact
    25561 aagcgttagt tgcgccgcgc acgtttcgca ggcaaatagc gtagaatgtc agcaggacaa
    25621 aggaaggagc aaaagttgat acccgtagag cgtcgccaaa tcatccttga gatggtagct
    25681 gaaaaaggca ttgtcagtat tgctgaacta acggacagaa tgaatgtgtc acatatgacc
    25741 attcgtcggg atttacaaaa actggagcag cagggagccg ttgtgctggt gtccggaggc
    25801 gtccagtctc cgggacgcgt ggcgcatgaa ccttctcatc aggtaaaaac tgcgctggca
    25861 atgacgcaaa aagcggctat tggcaagctg gcggcaagtc ttgttcagcc gggaagttgt
    25921 atctatctgg atgcgggaac gaccacgtta gcgatagcac agcatctgat tcacatggag
    25981 tcactgactg tggtcacaaa cgatttcgtt attgcagact acttgctcga caacagtaat
    26041 tgcacaatta ttcacactgg cggtgcagtg tgtcgggaaa accgttcctg tgtcggggaa
    26101 gccgctgcga ccatgctgcg cagcctgatg attgatcagg cttttatttc tgcatcgtca
    26161 tggagtgtgc gggggatttc tacgccagcg gaagataaag tcacggtgaa acgggcgatt
    26221 gccagtgcca gccgccagcg agttttggtc tgtgatgcga cgaaatatgg tcaggtggcg
    26281 acatggctgg cgttaccatt aagcgagttt gatcagatta tcacagacga cggtttgccg
    26341 gagagtgcca gtcgcgcgct ggcgaagcag gatctctctt tgctggtagc gaaaaatgaa
    26401 taatggcctg caataacatt tggttactca tgcttcacag aagaagcatg agactacttt
    26461 attttataaa atgacagccg cccgcttttc ggcgtgccgg tatcaatata aatctggtta
    26521 gcgaacgtct gaatgttatc aaacatcata tgtccaaata taaaataatc agcgccgttt
    26581 atttgttgta actcgccatt aagcgatttc tgcacacgat caacaggcca gagtaattcg
    26641 ctttctacta tttctttacc aaactgatat tcatcacccg gataatctgc atgtgcgatg
    26701 acatatttta tgttgtcatt aatgatttca ataatatgtg gaaggtgatg gagtttcagc
    26761 aacagatcta ttgcctcttg ttgctctgaa tcatttaaat cgaaaaacca gtcaccaccg
    26821 ctggcaagcc acatatttcc atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg
    26881 ttgcctttaa ccggcgtaaa ccagggctgg tttagcaggc gcagcacgtt aagactctcc
    26941 ggcccacgat cgatgttatc gcctaccgaa ataagtaagt cggtttcagg gcaaaaagag
    27001 agttgatgta agcgggattg taataattga tattcaccat gaatatcacc aacgacccat
    27061 atatggcgat agtgatgggc attaattttt tgatagcgtg tagatggcat ggttttaccc
    27121 tgtaaaataa gctttcctat tatacagggt gtttttattt tattcgtcag ttgtcgttaa
    27181 tattcccgat agcaaaagac tatcgggaat tgttattaca ccaggctctt caagcgataa
    27241 atccattcca gcgcctgacg cggagtcagt gaatccgggt ccaggttttc cagagcttcg
    27301 accgctggcg aggtttcttc cggtactgac agcaaagaca tttgcgtacc atccacttgc
    27361 gtagcggcgg cgttcggcga aatgctttcc agctcacgca gtttttgccg tgcgcgctta
    27421 atcacctctt ttggcacgcc tgccagagct gcaaccgcca agccgtagct tttgctcgcc
    27481 gcgccatcct gcacgctgtg cataaaggca atggtgtcgc cgtgctccag tgcatcgaga
    27541 tgcacgttgg cgacgccttc cattttctcc ggtaactgtg tcagctcgaa atagtgggtg
    27601 gcaaacagcg tcaacgcttt aatcttattc gccagatttt ccgcgcacgc ccacgccagc
    27661 gacagaccat cgtaagtgga agttccgcgc ccaatctcat ccatcagcac cagactgtac
    27721 tcggtggcgt tatgtaaaat attggcggtt tcagtcatct ccaccataaa ggttgagcgc
    27781 ccggaagcca ggtcatctgc cgcgcctacg cgggtaaaga tacggtcaat cgggccaatc
    27841 tcgacttttt gcgccggtac gtagctgccg atataggcca tcagcgcaat caacgcggtc
    27901 tggcgcatat aggtactttt accgcccatg ttcggaccgg tgatgatcaa catacggcgc
    27961 tgcggcgaca gattcagcgg gttagcgata aatggctcat tcagtacttg ttcaactacc
    28021 ggatggcgac cttcggtaat gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag
    28081 ttcagggtat aggcccgttc cgccaggtta accagcacgt cgagttccgc cagcgcgctc
    28141 gcgctctgtt gcaacgcttc cagatgcggc aacagcaggt cgaacagctc ttcataaagc
    28201 tgtttttcca gtgccagtgc tttgcctttt gaggtgagaa ctttatcttc gtactctttt
    28261 agctctggaa tgatgtagcg ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg
    28321 ggtgccagat ggctttgccc acggctgatt tgaatgtagt agccgtgcac cgcattaaag
    28381 ccaactttca gcgtgtccag gccggtacgt tcacgctcgc ggacttccag acgctccaga
    28441 taatcggtcg cgccgtcagc cagcgcgcgc cactcatcca gctcttcgtt atagcccgat
    28501 gcgataacac caccgtcgcg taccagcacc ggcggtgtgt cgatgattgc tcgctccagc
    28561 agatcgcgca gctcggcaaa ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta
    28621 tcgacagttt ctaactgcgc acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg
    28681 gccagatcgc gtgggcgagc agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg
    28741 ccgacctgac gcagtaccgg ctgtagcccg gcggtgaaat cctgcaatgc gccaatagtt
    28801 tgctggcgct caagcaacac gcgggtatcg cgcactggca tatgcagcca gcgtttcagc
    28861 atacggctgc ccatcggcgt gacggtgcag tcgagcacag aagccagcgt attttccgca
    28921 ccacccgcca ggttctgggt gatttccaga ttacgacgcg tcgcggcatc cataatgatg
    28981 ctgtcctgct cacgttccat ggtgatggaa cgaatatgcg gcagagtcgt acgttgggta
    29041 tctttcgcat actgcaacag acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg
    29101 ccaaaaccga ccagatcgcg ggtcccaaat tgcagattca actgctggcg cgcggtgtcg
    29161 atttcaaact cccacagcgg gcgacggcgc aggccgcgac ggccttcaat taacgacatt
    29221 tcagcaaaat cttctgcata cagcagttcc gcaggattag tgcgttgcag ttctgccgcc
    29281 atcgtttcgc ggtcagccgg ttcgctcagg cgaaaacgcc cggaactgat atccagcgtc
    29341 gcgtagccga aacctttgct gtcctgccag atagccgcca gcaggttgtc ctgacgctcc
    29401 tgcaacaggg cttcatcgct gatggtgcct ggcgtaacga tacgcacaac tttgcgctca
    29461 accggacctt tgctggtcgc cggatcgcca atttgttcgc agatggcaac ggactctccc
    29521 tgattcacca gtttggcgag atagttttcc accgcatggt agggaatccc cgccatcggg
    29581 atcggctctc ccgccgaagc accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg
    29641 cgttttgcgt cgtcataaaa cagttcataa aaatcaccca tccggtaaaa cagcaggatc
    29701 tcgggatgct gggctttcag cctgagatac tgctgcatca tgggcgtatg ggcgtcgaaa
    29761 ttttctattg cactcatggg gttatgtccg gttccctgat attaaatggg gtgtgatggt
    29821 ttttattttc gttgtaatca cataaggcgc acatgatacc ggagttaatc atttcagggg
    29881 aagaagcgaa cggcgagttc ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt
    29941 gcgtgatgct tatgaagcaa gtacgcaaaa ttgtatcggt tggctaaccc ttcttctttg
    30001 gagtcatagt gccctttaag attaagatgt aatataaata catcttaatg aggtggaggc
    30061 agcatgtccg gcaagcgtat ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt
    30121 tatcaagcga aatgcccgca ggcgtcagcg gagccggagc attacgaggc gttgtttgtt
    30181 tacgcgcaaa agcggctgga taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag
    30241 tgtccggtgc actgttacca gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg
    30301 gccgggccga gaatgctgtg gcgtcatccg atcttaaccg tgcgtcatct catcgatgac
    30361 aagcgtccgg tgccagaact gccggaaaaa tatcgtccga aaaaacctca tgaatagtat
    30421 gccggacagg cagatctgtc cggcaatttg cagttaaatc aatgccgatt tatcaattcc
    30481 cagccgcttc atccgtgaca gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc
    30541 tttaggcccg gcaaccacgc cgttggtttc tttcagcacg cgcacaatca actgatattc
    30601 atcttcgccc tccagggcga caaccgttgc ggcaggcggc gtttcaggtt ccggtaaaac
    30661 aatatctggc aatgacagct gcagcacgtt accgcgtgtt agcaataccg cgcgctcaat
    30721 gacgttttcc agttcgcgta cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt
    30781 ctcggcagga atgctgtcga tattgcgccc cagacgacgg gcaattttga aggtaaaggc
    30841 tttcgccagc agcggaatat cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa
    30901 tacgttcagg cggtaataga gatcgctacg gaactcacgg tcggcgacca tttttttcag
    30961 atcgcggtta gtcgcggcga ttagacgcac gtccgtctga atgattttgt tgctgccgag
    31021 acgttcaaac tcctgttcct gcaatacacg cagcaacttc ggctgtaact ccagtggcat
    31081 atcgcccact tcgtcgagga acagggagct tttatccgcc agttcaaaac gaccgatacg
    31141 ctgggcgctg gcaccggtaa aagccccacg ctcatgacca aacagatcgc tttccagcaa
    31201 tccggcaggc atcgccgcgc agttcatttt gaccatgcgg cgattattac gcccactgag
    31261 attatggatc gcacgggcaa tcagctcttt acccgtgcca gtttcaccga ggatcagcac
    31321 ggtactgtca ctttgcgcca ccatttcaac ttgtttaagc acgctgtaca tggcttcgct
    31381 gcggccaata atctcgccaa attcactatc aacattgttg agctgctcgg tcagggcgag
    31441 gttttcatca accagccgtt ctttcagacg atggatttcc tgataggcga gggcgttatc
    31501 gacagcgatt gccacacgtt cggcaatctg gcgcagtaaa ttcagattgg tagtggtaaa
    31561 cactttctct tcgcattgcg ccagtttcag cacgcccagc atggtgtcgc cagacatcag
    31621 cggtaacagg cacaaggttt gaatctggtt gccccaggtg tcgaacaaca tgcgttcata
    31681 gggggctaaa tcgtcccgct cgtggagatt gatcagcagc atctctttac ttttgaacac
    31741 gcgttcggtg agggttccgg cttcatcgac ttcgctctgt tcgtgggcgg gatgctgttt
    31801 atcaagatag tgagtggagt agatgttgag tttgttttta cggtggctgc gtaagacgat
    31861 actgatatcg tcaatgtcga aatagtaatg gatttctttg gcgacttcgc tgaccagttc
    31921 gtccatatcc aggcgggaaa gcaccgcgtt ggtgatggcg accaggatgc ggaagttatc
    31981 gcgttcccgg cataacaact catagtcgac attgttaacg acgcggctct ggatttgttc
    32041 ggtgacgaca gaaacgatct gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca
    32101 ggggcgatcg tcataacgaa taaattcaca gccaccaaaa atatgccctt ccgccgccag
    32161 tggcatcagg caatagtgac caaattttgg gtatagccca cctgcgtcca gctgcggcca
    32221 ggtttcacaa aattcttcgt aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg
    32281 taccggaccg tgtgccagaa cagtttcgtc ttcatattta atgggggtgt ctttttcacg
    32341 cgacgcgtaa taagacgcac gttgagtctg cgcttgccac aacacaatag ccgcgttgtc
    32401 ggcgagcgca gaacgcttta ccagttgcga aagagcctca cacagcgagg ccagatcggg
    32461 ctgctgcaat agtgtccgag tgatgtcgaa caacccttgt tgtccgagat cactcatcgg
    32521 tgtatatgac attttgttct tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac
    32581 taactatagg agatttagaa ttttattagc atatacgcgg aagcggttcg gcgtgtggta
    32641 aatcgagggt tcgtttcacg ccatacagac cggcaagacg aacaccttta cgttccacca
    32701 cttcaccaat cagcgccgcg tctttcccca gtggatggga atgtaacgct gccagcactt
    32761 gctctgccgc gttgcgttca acagctatta ctagtttgcc ttcgttggca aagttcaggg
    32821 cgtccagtcc cagcaattcg caaacgccac gcacggcagg tttaacaggc agtgccgctt
    32881 ctgaaagttc aataccacaa ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac
    32941 caccacgggt ggcatcacgc agcgctttca cgccgggaat gtcacgcagc gtctgaataa
    33001 gcggcgtcag caccgcgcag tcgctgacca gttcgccatc cagccccagc tgctcacgca
    33061 ggttaaggat agtcgccccg tggtcgccga gtgtaccgct caccagcaat acatcgcctg
    33121 cggttagcgt ctgtgcgccc cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt
    33181 tgataaacag tttatctacc gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg
    33241 caatgcctgc cgcgcgggcg gtttctgcca tgctggtcac tacggctttc agtgtctcca
    33301 tcggcaatcc ttcttcgagg ataaagccac aggagagata gcgcggaata gcgccactga
    33361 ccgcaacgtc attggctgtg ccgcaaatcg ccagcttgcc gatattaccg ccagggaaga
    33421 acagcgggtc aataacgtaa ctgtcggtgg agaacgccag acggtcgcct tccgctacca
    33481 gctgcgccag atcaagacgt gcctgatctt cctgctctgc cagccacggg ttggcaaagg
    33541 cttccataaa caggctgttg attaattgct gcatcgcctg gccgccgcta ccgtgggcga
    33601 gttggatatt attcacgctt cactctcctg ctgacgatac tgataccacg cggcgcacgc
    33661 tccttcggag gaaaccatca gcgcaccaaa cgcggtttga ggattacagg tgttaccaaa
    33721 cagcgggcat tgatgcggct tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg
    33781 gtcatcgcag acctgctgcg gtgccgggcg gaaatgtgct tcggcatcga atcgttgata
    33841 atccggcgtc aggtgcacgc cagaagattc aatcacgcct aagccgcgcc attcgctgtc
    33901 gccgttgaca cagaacacat cggcaatcgc ctgttgcgcc agcaggttac cggcatccgg
    33961 taccactcga cgatactgat tctctacctt gctgtgggcc gctattttct gctgcaccag
    34021 catgaccacg ccttgtagta gatcaagggg ttcgaatcca gcaaccacca gcggacgatg
    34081 aaaatcgctg gcgataaaat tataggcgtc ggtgccgata accatactga cgtgacccgg
    34141 cgcgaggaac gcatcgatac cgttatccgg ctgttccagc aaactgcgca acgtcgggat
    34201 aagcgtaatg tgctggcaga agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg
    34261 cagagtgata gcggtggtcg gcatagtggt ttcaaaacct aagccgaaga acaccacttt
    34321 gcgggttgga ttctcctgcg ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac
    34381 atcggcatcg cgtgcttttg cctgcaacag cgatccctgt ttccccggca cgcgcatggc
    34441 gtcgccaaag gtacagaaga tgacttccgg atggctggca atctccacgc aggtgtcgat
    34501 tctacccatc ggcagtacgc acaccgggca ccccggaccg tggataaact caacgttttc
    34561 cggcagtaac tggtcgaggc cgaatttaaa gatagcgtgg gtatgaccgc cacacacttc
    34621 cataatccgc agagggcgtt cggcggtgta agagagatgt gaagcacgtt cgcgcagatg
    34681 ctcaattaac tgcatcacct gttccggcgc gcgatattca tcaacaaaac gcattatttt
    34741 tcctcgccat acaacagcgc gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg
    34801 tcgagagtgt cgcgtgcttc ggcttcatta attacgctca tggcaaagcc aacgtgtacc
    34861 agtacccact ggcccacgcg cggctgaccg ttttcatcgc agctgccgac taacgttaaa
    34921 tcgacatcgc gctgaatgcc gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg
    34981 atctggccgg gaacgcctat gcacatcgct gtgtctccag ccagttcagc cactggtcca
    35041 tcccttcgcc gctggtggcg gaaataagga tgatttcaat ttctggattg acttcgcggg
    35101 cgcaggcgat gcacttttca acgtcaaagt tgagatacgg caacaggtca actttgttga
    35161 gcagcatcag cgaggcggcg gcaaacatat gcggatattt cagcggtttg tcttcacctt
    35221 cggtaacgga aagcaccgcc actttgtgtt tttcaccgag atcgaagctg gccgggcata
    35281 cgaggttgcc aacgttttcg ataaacagaa taccgttatc gtccagtggc agacgcggtg
    35341 cggcgtcggc aatcatctgt gcgtcaagat ggcagccttt accggtgttc acctgaatcg
    35401 ctggtgtgcc ggtagcgcga atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa
    35461 taactgcgca cggaacgctg tctttcaggc gcattagggt ttccgtcagc agggtggttt
    35521 taccggaacc agggctggaa accaggttga gcaccagttg cttgcgggca gcaaagcgcg
    35581 cgcggttgcg ttcagccaga cggttatttt tgtccagcac gtcaatttcg acttccagca
    35641 tccgacgctg gctcatgccc ggtgcgtgag tgcccgcttc gccatgaccg taatgcaggt
    35701 cgccttcttc agtctggctg ggggtaaatt caggcgcttt aatgccggtg attttcatct
    35761 tcgggcgtgc cgccggggca aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat
    35821 caccctcgat atacaggttg ccttcaccgc aaccgcatgt tgtacacatc gctcactcct
    35881 ggtctatttc tatccgccga atctgtaaac cgtcgtctgc cacaatctgc agcatgtcac
    35941 catgacactg tggacagcgg cggacgcgct gggtcagtag cgtcacatac tgttggcatg
    36001 tttcacacca gcattcggcc tcttgttctt cgaggtgcag tttacaacct tccgccacgc
    36061 tgccgcggca aaccagatca aaacaaaagg caagagagct ggtttcgaca caagaaaatg
    36121 cgccaatttt gagccagacc ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt
    36181 cgatcaattc cagtgcccgt tggcagaggg ttatttcgtg catatcgcct cccattaact
    36241 attgccagct acaagcaata attgtgccag tgttgattat ccctgcggtg aataatgtcg
    36301 atgatgtcga aatgacacgt cgacacggcg acgaaattca tctttagctt aaaaatctct
    36361 ttaataacaa taaattaaaa gttggcacaa aaaatgctta aagctggcat ctctgttaaa
    36421 cgggtaacct gacaatgact atttgggaaa taagcgagaa agccgattac atcgcacagc
    36481 ggcatcgtcg cctacaggac cagtggcaca tctactgcaa ttcgctggtt caggggatca
    36541 cgttatcgaa agcgcgcctg catcacgcca tgagctgcgc gccggacaaa gaactctgtt
    36601 tcgtcctttt tgaacatttt cgcatttacg tcaccctggc ggatggcttt aacagccaca
    36661 ccatcgagta ttacgtcgaa acaaaagata gcgaagacaa acagcggatt gcgcaggcgc
    36721 aactgagcat tgacggcatg attgatggca aggtcaacat ccgcgatcgc gaacaggttc
    36781 tggaacacta tctcgaaaaa atcgctggcg tttacgacag cttatacacc gctattgaaa
    36841 acaatgtgcc ggtgaattta agccaactgg taaagggaca aagcccggca gcatgagctg
    36901 aggctttgcc cgttttgcag gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc
    36961 gaaaatgaca tttcatcggc atgttttcgt caaaaatgac aatcacctga ggaatgcctg
    37021 gtgaatcgtt ttgtaattgc tgactccacg ctctgtatcg gctgccacac ttgtgaggcc
    37081 gcctgttcag agacgcatcg ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg
    37141 ctgaatgaaa aagaatctgc gccgcagctc tgtcaccact gtgaagatgc accctgcgcg
    37201 gtggtctgcc cggttaacgc catcacccgc gttgatgggg ccgtgcagtt gaatgaaagc
    37261 ctgtgcgtaa gctgcaagct gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc
    37321 ggcagccgtc cgctggatat tccggcaaac gccaataccc cgaaagcgcc accggcaccg
    37381 cctgctccgg cgcgtgtcag cacattgctt gactgggtgc caggtattcg cgcgatcgcc
    37441 gtcaaatgtg acctttgtag ctttgatgaa caaggtccgg cctgcgtgcg gatgtgcccg
    37501 actaaagccc tgcatctggt ggataacact gatatcgccc gcgtcagcaa acgtaagcgt
    37561 gagctgactt ttaacacgga ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga
    37621 gaggctaaat gagcgcaatt tccctgatca atagcggcgt ggcgtggttt gtcgccgccg
    37681 ctgttctggc atttctcttt tcttttcaaa aagcgttaag tggctggata gctggaattg
    37741 gcggcgcggt tggtagtctg tatacggcag ccgcgggctt cactgtactg actggcgcgg
    37801 ttggcgtgag cggtgcgctg tcgctggtaa gctacgatgt gcaaatttct ccgcttaacg
    37861 cgatttggct gattacgctc ggtctgtgcg gtctgtttgt cagcctctac aacattgact
    37921 ggcatcgcca cgcgcaggtg aagtgcaacg gcttgcagat caatatgttg atggctgccg
    37981 ccgtctgcgc cgtcattgcc agcaacctcg gcatgttcgt ggtaatggcc gaaatcatgg
    38041 ccctgtgcgc ggtgttcctc accagcaaca gcaaagaggg caaactgtgg tttgcgctgg
    38101 ggcgtcttgg cactctgctg ctggcgattg cttgctggct gctgtggcag cgttatggca
    38161 cgctggatct gcgcctgctg gatatgcgta tgcaacagct gccgctcggt tccgatatct
    38221 ggctgctcgg tgtgattggc tttggcctgc tggccgggat tattccgctg cacggctggg
    38281 tgccgcaggc acatgctaac gcctctgcgc cagctgccgc gttgttttct acggtagtca
    38341 tgaaaattgg cctgctgggc attttaaccc tgtcactgct gggcggtaat gcaccgctgt
    38401 ggtgggggat cgcgctgctg gtgctcggca tgatcaccgc gttcgtcggt ggtctgtatg
    38461 cgttgatgga acacaacatc cagcgcctgc tggcttacca caccctggaa aatatcggca
    38521 ttatcctgct ggggctgggc gctggcgtaa cgggtatcgc gctcgaacaa ccggcactga
    38581 ttgctcttgg cctggtcggt ggtctgtacc atctgcttaa ccatagcctg ttcaaaagcg
    38641 tactgttcct cggagcgggg agcgtctggt tccgtaccgg tcatcgcgat atcgaaaaac
    38701 tcggtggtat tggcaagaaa atgccggtta tctccatcgc catgttagtc gggctgatgg
    38761 caatggctgc gctgccgccg ctgaatggtt ttgccgggga atgggttatc tatcaatcct
    38821 tcttcaaact gagcaatagt ggcgcgtttg ttgcccgtct gctggggccg ctgctcgccg
    38881 tgggtctggc aattaccggt gcgctggcgg tgatgtgtat ggcgaaagtc tatggcgtca
    38941 cgttcctcgg cgcgccgcgt accaaagaag ccgaaaacgc cacctgtgca ccgctcctga
    39001 tgagcgtaag cgtagtggca ctggcgattt gctgcgtaat tggcggtgtt gctgcgccgt
    39061 ggctgctgcc gatgctgtct gctgctgtac ctctgccgct ggagcctgct aacaccaccg
    39121 tttctcaacc gatgatcacg ttgctgctga ttgcctgccc gctgctgcca ttcatcatta
    39181 tggcgatttg caaaggcgat cgtttgccat cgcgttcccg cggcgcggcc tgggtgtgcg
    39241 gctacgacca cgaaaaatca atggtgatta ccgctcacgg ttttgccatg ccggtgaaac
    39301 aggcgtttgc gccagtgctg aaactgcgca aatggctgaa tccggtgtcg ctggtgccgg
    39361 gctggcagtg cgaaggtagt gcgttgctgt tccgccggat ggcgctggtt gaactggcgg
    39421 tactggtggt gattattgtt tcacgaggag cctgagaatg agtgttttat atccgttaat
    39481 tcaggcgctg gtgttatttg ccgttgcgcc gctgctctcc ggtattaccc gcgtggcgcg
    39541 cgcccgtctg cataaccgtc gtgggccggg tgtgttgcag gagtatcgtg acattttcaa
    39601 actgctgggc cgccagagcg ttggcccgga tgcctccggc tgggtgttcc gcctgacgcc
    39661 gtatgtgatg gtgggcgtca tgctgactat cgctactgcg ctgccggtgg tgaccgtcgg
    39721 ttctccgctg ccgcaactgg gtgatttaat caccttactg tatctctttg ccatcgcgcg
    39781 tttcttcttt gccatttctg gtctggatac cggtagcccg tttaccgcta tcggcgcgag
    39841 ccgtgaagcg atgcttggcg tgctggtcga accgatgctg ctgcttggtc tgtgggttgc
    39901 cgcacaggtt gccggttcca ccaacatcag caacatcacc gacaccgttt atcactggcc
    39961 gctgagccag agcatcccgc tggtactggc gctttgtgcc tgtgcgttcg ccacttttat
    40021 cgaaatgggc aaactgccgt tcgacctggc ggaagccgag caggagttgc aggaaggtcc
    40081 gctctctgag tatagcggca gcggctttgg cgtcatgaaa tggggtatca gcctgaaaca
    40141 gctggtggtg ctgcagatgt tcgtcggggt gtttattccg tggggacaaa tggaaacctt
    40201 caccgccggt ggactgctgc tggcgctggt gattgccatc gtaaaactgg tggtcggcgt
    40261 cctggttatc gcgctgttcg aaaacagcat ggcccgtctg cgtcttgata ttactccgcg
    40321 cattacctgg gctgggtttg gctttgcatt tttagcgttc gtctccttgc tggcggcgtg
    40381 attaaagaga gtttgagcat gtctgaagaa aaattaggtc aacattatct cgccgcgctg
    40441 aatgaggcat ttccgggcgt cgtgctggac cacgcctggc agaccaaaga tcagctgact
    40501 gtcaccgtaa aggtgaacta cctgccggaa gtggtggagt ttctttacta caaacagggt
    40561 ggctggctgt cggtgctgtt tggtaacgac gaacgcaaac tgaatggtca ttacgccgtt
    40621 tactacgtgc tgtcgatgga gaagggcact aagtgttgga ttacggttcg cgtcgaagtt
    40681 gacgccaaca aaccggaata tccgtccgtg acaccgcgcg ttccggcggc ggtgtggggc
    40741 gagcgcgaag tgcgcgatat gtacggtttg attccggttg gtctgccgga tgaacgccgt
    40801 ctggtgctgc cggatgactg gccggatgaa ctttatccgc tgcgtaaaga cagcatggat
    40861 tatcgtcagc gtccggcgcc gaccaccgat gctgaaacct acgagttcat caacgaactg
    40921 ggcgacaaga aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc cgacgaaccg
    40981 ggccacttcc gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgcctgttc
    41041 tacgttcatc gcggtatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc
    41101 ttcctctctg accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg
    41161 tcggtagaaa acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt
    41221 ctgctggagg tggaacgcct gcactcacat ctgctcaacc tcggtctcgc ctgccacttt
    41281 accggctttg actccggctt tatgcagttc ttccgcgtgc gtgaaacctc tatgaaaatg
    41341 gcggagatcc tcaccggcgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt
    41401 cgcgatctgc tgaaagatga catgatccag acccgtcaac tggcgcaaca gatgcgtcgt
    41461 gaagtgcagg agctggtgga tgtgctgttg agcacaccga acatggaaca acgcactgtc
    41521 ggcattggtc gtctggaccc ggaaattgcc cgcgacttca gtaacgtcgg cccgatggtc
    41581 cgcgccagcg gtcacgcccg cgatacccgt gccgatcacc cgtttgtcgg ttatggcctg
    41641 ctgccaatgg aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt
    41701 atcaacgaag tctataccgc gctgaacatg atcgactacg gtctggataa cctgccgggt
    41761 ggcccactga tggtggaagg ctttacctac attccgcacc gctttgcgct gggctttgcc
    41821 gaagcgccgc gcggcgatga tatccactgg agcatgaccg gcgacaacca gaagctgtac
    41881 cgctggcgct gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc
    41941 ggcaacaccg tttccgatgc gccgctgatt atcggtagcc tggacccttg ctactcctgt
    42001 accgaccgca tgaccgtagt cgatgtgcgt aagaagaaga gcaaagtggt gccgtacaaa
    42061 gaactcgagc gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg
    42121 tttaccttta tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag
    42181 ccgattgcgg ttgataaaaa cttccgtggt aagccagagc agaacccgca gcagtgcatc
    42241 ggctgcgcgg cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc
    42301 gccacaggag agcttgcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc
    42361 gaagaagttt gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcggtgtgg
    42421 aaaaaagaag atttcctgca acagtcccgc tttgcgctgt gcaactgtcg cgtctgcaat
    42481 cgtcctttcg ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc
    42541 gacagccgcg cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag
    42601 aaatgcctgg tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga
    42661 tgagcaattt attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg
    42721 aatccatcgc cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctatgttt
    42781 accgcgtgga ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc actttatcgc
    42841 cgctgtttga tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata
    42901 ttttactgtt taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc
    42961 agtccgcgcc ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta
    43021 ttttccacga tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct
    43081 acattccggg ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcactcg
    43141 gcctgctgga gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caaccggctg
    43201 agattctgca tggcgatatg gtgcagccgc tgcgcgtgaa agtggatcgc gaagcacgtc
    43261 gcctggcggg ttatcgttac ggtcgtcaga ttgccgatga ttaccttaca cagttagggc
    43321 agggcgaaga acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga
    43381 ttgtcagcca tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt
    43441 gttcagtcaa ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca
    43501 gcaggtggtc tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga
    43561 agcggcgctc acctgcccat gggatgaata tctggcatgg attgccactc tggaggcggg
    43621 cagcgaagcc cgccgcaaaa tggaaggcgt accgaaatat ggcgagatcg tcatcgacat
    43681 taaccatgtg ccgatgctgg ccaatgcatt cgataaagcc cgggcagcgc aaacttcgca
    43741 gcagcaggaa tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc
    43801 catctatttg atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca
    43861 tgatgggcga tgatggcgca ggtccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag
    43921 gtaactgggt ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg
    43981 aactgcgccc tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg
    44041 aaatccgcat catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca
    44101 tgcctctgaa ctatcttatc gaccagttga aagaagatat tggcgaagtg atttttctcg
    44161 gcatccagcc ggatatcgtc ggcttttact acccgatgac ccagccgatt aaagatgcgg
    44221 ttgaaaccgt ttatcaacgt ctggaaggct gggaagggaa tggcggtttc gcgcagttag
    44281 cggtagaaga agagtagttt ttcagtaagg aatcaggaca gggatgttct tgatgaggga
    44341 atcagaactg atagcaaacg ccagattgcc cga
//
LOCUS       DACAPB010000009        44056 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.009, whole genome shotgun sequence.
ACCESSION   DACAPB010000009 DACAPB010000000
VERSION     DACAPB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.009"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..2431
                     /locus_tag="IT904_000466"
     CDS             <1..2431
                     /locus_tag="IT904_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAP3190301.1"
                     /translation="EKGQKITPGVYRVDIVLNQTIVDTRNVNFVELTPEKGIAACLTT
                     ESLDAMGVNTDAFPAFKQLDKQACALLAEIIPDASVTFNVNKLRLEISVPQIAIKSNA
                     RGYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNN
                     STWSRSSGQTAEWKNLISYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDN
                     MLPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLV
                     EIKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGG
                     PWGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFL
                     YAKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLF
                     YTKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGGSWN
                     YSKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNL
                     GLTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLN
                     YALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATS
                     YRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLR
                     FGAIATLDGVQANSGIIDDDGSLYMAGLPAKGTISVRWGEAPDQICHINYELTEQQIN
                     SAITRMDAICR"
     gene            2422..3492
                     /locus_tag="IT904_000467"
     CDS             2422..3492
                     /locus_tag="IT904_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3190302.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEANTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQCLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3504..4046
                     /locus_tag="IT904_000468"
     CDS             3504..4046
                     /locus_tag="IT904_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3190303.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4054..4569
                     /locus_tag="IT904_000469"
     CDS             4054..4569
                     /locus_tag="IT904_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3190304.1"
                     /translation="MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4535..5272
                     /locus_tag="IT904_000470"
     CDS             4535..5272
                     /locus_tag="IT904_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAP3190305.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            5383..6393
                     /gene="pyrD"
                     /locus_tag="IT904_000471"
     CDS             5383..6393
                     /gene="pyrD"
                     /locus_tag="IT904_000471"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAP3190306.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6567..7109
                     /gene="zapC"
                     /locus_tag="IT904_000472"
     CDS             6567..7109
                     /gene="zapC"
                     /locus_tag="IT904_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAP3190307.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSFEQAV"
     gene            complement(7106..8215)
                     /gene="ycbX"
                     /locus_tag="IT904_000473"
     CDS             complement(7106..8215)
                     /gene="ycbX"
                     /locus_tag="IT904_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAP3190308.1"
                     /translation="MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            8459..10567
                     /gene="rlmKL"
                     /locus_tag="IT904_000474"
     CDS             8459..10567
                     /gene="rlmKL"
                     /locus_tag="IT904_000474"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAP3190309.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYTNRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10579..12486
                     /locus_tag="IT904_000475"
     CDS             10579..12486
                     /locus_tag="IT904_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3190310.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12616..13869
                     /gene="pqiA"
                     /locus_tag="IT904_000476"
     CDS             12616..13869
                     /gene="pqiA"
                     /locus_tag="IT904_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAP3190311.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13874..15514
                     /gene="pqiB"
                     /locus_tag="IT904_000477"
     CDS             13874..15514
                     /gene="pqiB"
                     /locus_tag="IT904_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAP3190312.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15511..16074
                     /gene="pqiC"
                     /locus_tag="IT904_000478"
     CDS             15511..16074
                     /gene="pqiC"
                     /locus_tag="IT904_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAP3190313.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16330..16497
                     /gene="rmf"
                     /locus_tag="IT904_000479"
     CDS             16330..16497
                     /gene="rmf"
                     /locus_tag="IT904_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAP3190314.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGHSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16567..17085)
                     /gene="fabA"
                     /locus_tag="IT904_000480"
     CDS             complement(16567..17085)
                     /gene="fabA"
                     /locus_tag="IT904_000480"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAP3190315.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17154..18914)
                     /locus_tag="IT904_000481"
     CDS             complement(17154..18914)
                     /locus_tag="IT904_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAP3190316.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19100..19552
                     /gene="matP"
                     /locus_tag="IT904_000482"
     CDS             19100..19552
                     /gene="matP"
                     /locus_tag="IT904_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAP3190317.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19628..20668)
                     /gene="ompA"
                     /locus_tag="IT904_000483"
     CDS             complement(19628..20668)
                     /gene="ompA"
                     /locus_tag="IT904_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAP3190318.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21025..21534)
                     /gene="sulA"
                     /locus_tag="IT904_000484"
     CDS             complement(21025..21534)
                     /gene="sulA"
                     /locus_tag="IT904_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAP3190319.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            21753..22382
                     /gene="sxy"
                     /locus_tag="IT904_000485"
     CDS             21753..22382
                     /gene="sxy"
                     /locus_tag="IT904_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAP3190320.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVACRQELAEWADSLTPKQEFPAELE"
     gene            complement(22345..24507)
                     /gene="yccS"
                     /locus_tag="IT904_000486"
     CDS             complement(22345..24507)
                     /gene="yccS"
                     /locus_tag="IT904_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAP3190321.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTFST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDQSQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(24517..24963)
                     /locus_tag="IT904_000487"
     CDS             complement(24517..24963)
                     /locus_tag="IT904_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP3190322.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25086..27140
                     /gene="helD"
                     /locus_tag="IT904_000488"
     CDS             25086..27140
                     /gene="helD"
                     /locus_tag="IT904_000488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAP3190323.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLISFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27172..27630)
                     /gene="mgsA"
                     /locus_tag="IT904_000489"
     CDS             complement(27172..27630)
                     /gene="mgsA"
                     /locus_tag="IT904_000489"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP3190324.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(27726..28388)
                     /locus_tag="IT904_000490"
     CDS             complement(27726..28388)
                     /locus_tag="IT904_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAP3190325.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPHLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28561..28974
                     /locus_tag="IT904_000491"
     CDS             28561..28974
                     /locus_tag="IT904_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP3190326.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29019..29336)
                     /gene="hspQ"
                     /locus_tag="IT904_000492"
     CDS             complement(29019..29336)
                     /gene="hspQ"
                     /locus_tag="IT904_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAP3190327.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29394..30584)
                     /gene="rlmI"
                     /locus_tag="IT904_000493"
     CDS             complement(29394..30584)
                     /gene="rlmI"
                     /locus_tag="IT904_000493"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAP3190328.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            30679..30957
                     /gene="yccX"
                     /locus_tag="IT904_000494"
     CDS             30679..30957
                     /gene="yccX"
                     /locus_tag="IT904_000494"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP3190329.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(30954..31283)
                     /gene="tusE"
                     /locus_tag="IT904_000495"
     CDS             complement(30954..31283)
                     /gene="tusE"
                     /locus_tag="IT904_000495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAP3190330.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31374..32033)
                     /gene="yccA"
                     /locus_tag="IT904_000496"
     CDS             complement(31374..32033)
                     /gene="yccA"
                     /locus_tag="IT904_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAP3190331.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32240..32327)
                     /locus_tag="IT904_000497"
     tRNA            complement(32240..32327)
                     /locus_tag="IT904_000497"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32291..32293),aa:Ser,seq:tga)
     gene            32754..33872
                     /gene="hyaA"
                     /locus_tag="IT904_000498"
     CDS             32754..33872
                     /gene="hyaA"
                     /locus_tag="IT904_000498"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAP3190332.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIDKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            33869..35662
                     /gene="hyaB"
                     /locus_tag="IT904_000499"
     CDS             33869..35662
                     /gene="hyaB"
                     /locus_tag="IT904_000499"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAP3190333.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNCLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            35681..36388
                     /gene="hyaC"
                     /locus_tag="IT904_000500"
     CDS             35681..36388
                     /gene="hyaC"
                     /locus_tag="IT904_000500"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAP3190334.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36385..36972
                     /locus_tag="IT904_000501"
     CDS             36385..36972
                     /locus_tag="IT904_000501"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAP3190335.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            36969..37367
                     /gene="hyaE"
                     /locus_tag="IT904_000502"
     CDS             36969..37367
                     /gene="hyaE"
                     /locus_tag="IT904_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAP3190336.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37364..38221
                     /locus_tag="IT904_000503"
     CDS             37364..38221
                     /locus_tag="IT904_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAP3190337.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38355..39899
                     /gene="appC"
                     /locus_tag="IT904_000504"
     CDS             38355..39899
                     /gene="appC"
                     /locus_tag="IT904_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAP3190338.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRGLEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            39911..41047
                     /gene="appB"
                     /locus_tag="IT904_000505"
     CDS             39911..41047
                     /gene="appB"
                     /locus_tag="IT904_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP3190339.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41060..41152
                     /gene="cbdX"
                     /locus_tag="IT904_000506"
     CDS             41060..41152
                     /gene="cbdX"
                     /locus_tag="IT904_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAP3190340.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            41232..42530
                     /locus_tag="IT904_000507"
     CDS             41232..42530
                     /locus_tag="IT904_000507"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAP3190341.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42645..>44056)
                     /locus_tag="IT904_000508"
     CDS             complement(42645..>44056)
                     /locus_tag="IT904_000508"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HAP3190342.1"
                     /translation="IARQAAQDSQSLEFLQRQLPEVRSELDQAEEKLNVYRQQRDSVD
                     LNLEAKAVLEQIVNVDNQLNELTFREAEISQLYKKDHPTYRALLEKRQTLEQERKRLN
                     KRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNRQQELSISKSSAIGNVRIIDPAVTQP
                     QPVKPKKALNVVLGFILGLFISVGAVLARAMLRRGVEAPEQLEEHGISVYATIPMSEW
                     LDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPADSAVEAVRALRTSLHFAMMETENNI
                     LMITGATPDSGKTFVSSTLAAVIAQSDQKVLFIDADLRRGYSHNLFTVSNEHGLSEYL
                     AGKDELNKVIQHFGKGGFDVITRGQVPPNPSELLMRDRMRQLLEWANDHYDLVIVDTP
                     PMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIKGAILNGVIKRA
                     STAYSYGYNYYGYSYSEKE"
BASE COUNT        10915 a        10806 c        11746 g        10589 t
ORIGIN      
        1 cgaaaaagga caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac
       61 aattgtagat acgcgcaacg tcaattttgt tgagttaacg ccagagaagg ggattgccgc
      121 ctgtttgacg actgaaagcc tggatgcaat gggtgtgaat actgatgcgt ttccggcttt
      181 taaacaactg gacaaacaag cgtgtgcgct attggcggag attattccgg atgccagcgt
      241 aacttttaat gtgaataaac tccgtctgga aatttcagta ccgcaaattg ctataaaaag
      301 taacgctcgt ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact
      361 gggatattca tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga
      421 cagttatttt ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa
      481 ttcaacatgg agtcgcagta gtggccaaac cgcagaatgg aagaatctca tcagctattt
      541 gcagcgggcg gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg
      601 cgattttttc gatagtgtca gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct
      661 gccagacagc ctgaaagggt ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca
      721 gataacgatt aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt
      781 tgaaattagt gatctctatt ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga
      841 agcggacggc agcgtcaata gctacagcgt accgttttcc agcgtgccat tactccagcg
      901 tcaggggcga atcaaatacg cggtgacact ggcgaaatac agaaccaata gtaatgaaca
      961 gcaggagagc aaatttgccc aggccacgtt gcagtggggc ggaccgtggg gaacgacatg
     1021 gtatggtggt ggacaatatg ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa
     1081 ccttggcgat ttcggagcaa tttcgttcga tgcgacccag gcgaagagta cgctggcaga
     1141 ccaaagcgaa cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt
     1201 gggcactaat tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc
     1261 cgacaccatg tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac
     1321 gccgatgtgg tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa
     1381 tatctcccag caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta
     1441 ctggcatacc gatcaacagg atcggctatt acagtttggc tacaacacgc aaattaaaga
     1501 tctctcgctg gggggttcct ggaactacag taagtcccgt ggtcaacctg atgctgatca
     1561 ggtgtttgca ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag
     1621 ctataccagg aaaaaaaatt acgcctggat gacctctaac accagtatcg ataacgaagg
     1681 gcacattaca caaaacctgg gtttaacgga gacactactc gatgacggta acctgagcta
     1741 cagcgtgcaa cagggatata acagcgaggg gaaaacggct aatggtagcg ccagcatgga
     1801 ctacaaaggg gcgtttgcag atgcccgagt gggctacaac tacagcgata acggcagtca
     1861 acaacaactg aactacgctc tttcaggcag tttagttgcc cattcacagg gcattaccct
     1921 ggggcaatcg ctgggggaaa ctaacgttct gattgcagca ccaggcgcag agaatactcg
     1981 tgtggcgaac agcaccgggc tgaaaactga ctggcgcgga tataccgttg ttccttatgc
     2041 cacttcttat cgggaaaatc gaatcgcact tgatgcggcg tcgttaaaac gtaacgtgga
     2101 tcttgaaaat gcagtagtca acgtggttcc caccaaaggg gcgttggttc tggcggagtt
     2161 caatgcccat gcgggtgcaa gggtattaat gaaaacatca aagcagggta taccgctgcg
     2221 ttttggcgcg atagcgacgc tggacggcgt acaggctaat agcggcataa ttgatgatga
     2281 tggctcgctc tatatggcgg gtttaccggc gaagggaaca ataagcgtgc gctggggcga
     2341 agctcccgat caaatttgtc atatcaatta cgagcttacc gaacaacaaa ttaactctgc
     2401 gattacgcga atggatgcca tatgcagata atctttggag aaaaatgcgt gtcattacta
     2461 cgactatttt ttgccgccgt cttaatgcta tggtgcgctc aaaccgctgc ttatagcggg
     2521 cagtgtcata ctactcaggg gaatccgtat attggcgtca attttggcgt taaaaccctg
     2581 gaggaagaag caaatacggc aggggtagtt aaagacaaat tttatcagtg gaacgaatcg
     2641 aatgattatt atgtttcctg tgattgcgat aaagacaatg tcagaagtgg ccgatgggca
     2701 ttcgccgcgg attcaccgtt agtctattta ggcgacaact ggtacaaaat taatgactat
     2761 cttgccgcca aagttttatt gcaggttaaa ggcagttctc ctactgcggt tcctttcgaa
     2821 aacgtgggca caggggggga tacccgatgg catatttgcg accctggcgg tcaatgttta
     2881 ggtgggcagg gggcaagcgg taatagcggt agcttttccc tgaaaatatt gcagccgttc
     2941 gttggctcgg tcgtcattcc tcctatggcg ctggcgcgat tatatgaatg ctacaacata
     3001 cccgcaggtg attcctgcac gactacaggt acaccggttt tagtgtatta cctgtctggt
     3061 acgatcaatt cacttggctc atgttccgtc aatgccggag agacaattga agttgattta
     3121 ggtgatgtct tcgctgccaa tttccgtgtt gtagggcata aacctcttgg ggccagaaca
     3181 gcagaacttg caattccagt caggtgtaac acgggaaacg cgggattagt taatgtcaac
     3241 ctgagtctga cggcaaccac agaccccagc tatccccagg cgattaagac gtcacgtcct
     3301 ggcgtgggcg tggtggtgac cgatagccag aacaacatta tttcccctgc tggtggaaca
     3361 ttaccgctct ctattcctga tgatgcagac agtatcgcgc gaatgaatgt ctatccagtc
     3421 agcacgacag gtgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata
     3481 aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atttcagtgc gttatcttgt
     3541 tctttagcat tcttaacatc catgtcggga tggctgggcc tgaacaagtt agtatgcata
     3601 tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata
     3661 ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta agcactgctg
     3721 ctgatttgaa tatcgatatc accggttgtg ccgctggtat tactggcgcg gacgtccttt
     3781 tcagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac acaggcggaa
     3841 gcggtggtat ggcaacgggg attgccgtgc aaattcttga tgcgcaaagt cagcaagaaa
     3901 tcccgctcaa tcaggtccag cctcttacgc ccttaaaagc cggggataac acactcaaat
     3961 atcaacttcg ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg acggcggttc
     4021 tctattttga tctggtttac cagtgaagga gtgatgttga aacggataat ctggattctg
     4081 ttcttattgg gattaacgtg gggctgtgag ctatttgccc atgatggcac ggtcaacatt
     4141 agcggatcgt ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca aatcaatgtg
     4201 cagttaggcg atgtctcgct gacacgtttt tctcatggga attatggccc ggagaaatct
     4261 ttcatcatta atttgcagga ttgcggaaca gatgtcagca cggttgacgt caccttttca
     4321 ggaacccccg acggagtgca gagcgaaatg ttgtccattg aaagtggtac ggatgcagcc
     4381 agcgggctgg caattgcgat tctggatgat gcgaagatat tgattccgct taaccaggcg
     4441 agtaaagact acagcctaca tagcggtaag gtgccattaa ctttttatgc gcaattgcga
     4501 cctgtcaata gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt tgtacttcat
     4561 tatgactaat acgtggaaca gattggcgct cttgattttc gccgttttat cgctgctggt
     4621 ggcgggtgaa ttgcaggcag gagtggtggt cggcggaacg cgatttatct ttccggcaga
     4681 cagagaatcg atatctattt tactgactaa taccagtcag gaatcctggc ttattaacag
     4741 taaaatcaac cgcccaacgc gttgggccgg gggggaagcg tcgacagtgc cagcaccatt
     4801 actggccgct ccgccactta ttctcctgaa gcccggtacg acaggcacgt tgcgcttgct
     4861 gagaacggaa agcgacatct tgcctgtgga tcgcgaaacg ctatttgagt taagcattgc
     4921 cagcgtgcca tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatgc gctcggtatt
     4981 taaactgttc tggcgacccg aaggtttgcc gggcgacccg ctggaagctt atcaacaatt
     5041 acgctggaca cggaattcgc agggtgtaca actcactaac ccaacgcctt attacattaa
     5101 cctgattcag gtgagtgtaa atggtaaagc gttaagtaat gtgggagttg tgccgcctaa
     5161 aagccagcgt cagacaagct ggtgtcaggc aatcgcaccg tgtcatgtcg cctggcgggc
     5221 gataaatgat tacggcgggt tgagtgcaaa gaaggagcaa aatctgccct gaaacaggtt
     5281 cggaaaacgt ttgcgttttt tttgccgcag gtcaattccc ttttggtccg aactcgcaca
     5341 taatacgccc ccggtttgca caccgggaat ccaggagagt tcatgtacta ccccttcgtt
     5401 cgtaaagccc ttttccagct cgatccagag cgcgctcatg agtttacttt tcagcaatta
     5461 cgccgtatta caggaacgcc gtttgaagca ctggtgcggc agaaagtgcc tgcgaaacct
     5521 gttaactgca tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg tcttgataaa
     5581 gacggggagt gcattgacgc gttaggcgcg atgggatttg gatcgatcga gatcggtacc
     5641 gtcacgccac gtccacagcc aggtaatgac aagccgcgtc tctttcgtct ggtagatgcc
     5701 gaaggtttga tcaaccgtat gggctttaat aatcttggcg ttgataacct cgtagagaac
     5761 gtaaaaaagg cccattatga cggcgtcctg ggtattaaca tcggcaaaaa taaagatacg
     5821 ccagtggagc agggcaaaga tgactatctg atttgtatgg aaaaaatcta tgcctatgcg
     5881 ggatatatcg ccatcaatat ttcatcgccg aataccccag gattacgcac gctgcaatat
     5941 ggtgaagcgc tggatgatct cttaaccgcg attaaaaata agcaaaatga tttgcaagcg
     6001 atgcaccata aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc tgaagaagaa
     6061 ttgatccagg ttgccgatag tttagttcgc cataatattg atggcgttat tgcaaccaat
     6121 accacactcg atcgttctct tgttcaggga atgaaaaatt gcgatcaaac cggtggctta
     6181 agtggtcgtc cgcttcagtt aaaaagcacc gaaattattc gccgcttgtc actggaatta
     6241 aacggtcgct taccgatcat cggtgttggc ggcatcgact cggttatcgc tgcgcgtgaa
     6301 aagattgctg cgggtgcctc actggtgcaa atttattctg gttttatttt taaaggtccg
     6361 ccgctgatta aagaaatcgt tacccatatc taattatttc ttcgaattcc tctttataag
     6421 ataatcaggg ccttattttc agccctggtt gttttatatt catctctgtt gcttatttag
     6481 acattttgta cttttattgt tgaggttatt aagcgaagcg acaatggatt gtgttgctgc
     6541 ggttttatag ggtaggggag aggcagatgc gaattaaacc agacgataac tggcgttggt
     6601 attacgatga agagcacgat cgaatgatgc tcgatttagc caatggtatg ctatttcgct
     6661 cacgttttgc gcgcaagatg ttgactccag atgccttttc tcccgcaggc ttttgcgttg
     6721 acgacgccgc gctctatttc tcttttgaag aaaagtgccg cgattttaat ttatctaaag
     6781 aacaaaaagc cgaactggtg ctgaatgcac tggtagcaat tcgctatctc aaaccgcaaa
     6841 tgccgaaaag ctggcatttt gtttcccatg gtgaaatgtg ggtgcccatg ccgggcgatg
     6901 cggcctgcgt ttggttgagc gatactcacg agcaggtcaa tttgctggtc gttgaatctg
     6961 gcgaaaatgc cgcactatgc ctgctggcac aaccctgcgt tgttattgcg ggtcgcgcga
     7021 tgcagttggg cgacgccatt aaaatcatga acgacaggct gaaaccgcag gttaatgttg
     7081 acagcttcag cttcgaacag gcagtctaac gcgccaactt aagtgcagtc ttcggtacac
     7141 agctacagca aagaatggtg ccatcatcgc ccattgctga ttttttcagc ggcgtgactt
     7201 cgccttctaa aagctgaaca cggcaacttc cacaaatgcc cgcgcggcaa gaataaggga
     7261 tacgaattcc ctgattttct aattgctcca gcaacacctg ttggttattt ccacgaaatg
     7321 cctgtccctg ccagtcaata tctacatttg cgtccggttg ttgcgtgatg ttggcggtat
     7381 catcagcggc agctgcgccg taaatttttg ccggagccgt tgccagaatt tccacctcat
     7441 cgccaacgcg aatcacgccg ctattacggg caattaaatt ctgaccaaaa tcgacatcgc
     7501 cgttatcctg ggcagtgcgg aaagattgca atgtttttaa tggttcgcct gccggatgtt
     7561 tttgcccttt ttctgggctg acggtggtga aaatacagcg gctacaaggt ttaaccacat
     7621 caaacaccac atcaccaatg cgaatcactt tccagcgatc ttcttcccag gctgacgcgc
     7681 cggaaaccac cagattgggg cggaattgct ccatttttac actggccgga caacgttgtt
     7741 ggagatcacg taacgaggct tcgttagcaa gaaggtaagg atagccatca gcaaatgaca
     7801 gaggtacagt gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc cagcgtaatt
     7861 gcacttcgcg ggagaaaaat ccacttagcc atttgttgat cgcgtctggc gcaattcgcg
     7921 cggtaaaatg tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa tcagcaaaac
     7981 gaacatatgc gctactgcca tctggtgcgg tgagatgcaa gccatcatgc acaggtgaag
     8041 gagtaaaccg taccatctgg ggaaactggc gagcggtaat aaacgtaccg tcaggttccg
     8101 tgatcataaa gatgcgatcg aaggccagac cactgacatc tgccagagca tgtgtaagac
     8161 caatgccgcg catcgattta acaggatgaa taaaaagccg gattaatgtc gccacggtgc
     8221 ggtcctcaaa tgaaaataag ccctcaactt tatgacatga cgcgcttatt agctataatg
     8281 cgcaacaatt ttcttagcgc ctgtcccatt aggctatttt atttgtcagt ttggccctgg
     8341 gttatgctcg aaatcctcac gtacttgtgt acgctccggt ttctccgcgc tgtccatgtc
     8401 cagactgact gcaacaatta cgcctactgc ggtaggctct tagagtaaaa gtgacgatat
     8461 gaattctctg tttgccagta cggcccgtgg gctggaagag ctgttaaaaa ctgaactgga
     8521 aaacctgggg gccgttgaat gccaggtggt tcagggtggg gtccatttca agggcgacac
     8581 acggcttgtt taccagagcc tgatgtggag ccgcctggcc tcgcgtatta tgttgccgct
     8641 gggcgagtgt aaggtttaca gcgatttaga cctctatctc ggtgttcagg cgatcaactg
     8701 gacagagatg tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt tgaatgacac
     8761 catccgcaac agtcagtacg gcgcgatgaa agtgaaagac gcgatcgtcg atgctttcac
     8821 gcggaaaaat ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc gcgttaacgt
     8881 ctggctgcat aaagaaaccg ccagtatcgc tcttgatctg agtggtgatg gtttacatct
     8941 gcgtggctat cgcgatcgtg ctggtattgc gccgatcaaa gaaaccctgg cagccgctat
     9001 tgtgatgcga tccggctggc agccaggaac accgctgctc gatccgatgt gtggttccgg
     9061 tacgttgctg attgaagcag cgatgctggc gaccgatcgc gcaccaggct tgcaccgtgg
     9121 gcgttggggc tttagcggct gggcgcagca tgatgaagct atctggcagg aagtgaaagc
     9181 ggaagcgcaa actcgcgccc gtaaaggcct ggctgagtat agctctcatt tttacggttc
     9241 ggacagcgac gcacgggtga ttcaacgtgc acgcactaac gcccgtcttg cggggattgg
     9301 tgaactgatc acctttgagg tgaaagatgt cgcgcaactg accaatccgc tgccgaaagg
     9361 gccgtacggc acagtgttga gcaacccgcc atacggggaa cgtctggaca gcgaaccggc
     9421 gctgattgcg ctgcatagcc tgctgggtcg gatcatgaaa aaccagtttg gtggctggaa
     9481 tctctctttg tttagtgcct cgccggatct gctaagctgc ttgcagctgc gtgcagacaa
     9541 acagtacaag gcgaaaaacg gcccgctgga ctgcgtacag aaaaattacc acgttgccga
     9601 aagcactcca gacagcaaac cggcgatggt agcggaagac tacaccaacc gcctgcgtaa
     9661 gaacctcaaa aaattcgaga agtgggctcg ccaggaaggg attgaatgtt accgcctgta
     9721 tgacgccgat ctgccagaat ataacgttgc cgttgaccgt tatgccgact gggtggtggt
     9781 gcaggagtat gcgccgccaa aaactattga tgctcacaaa gcgcgtcagc gtctgttcga
     9841 tattatcgct gcaaccattt cggtactggg gattgcgcca aacaaactgg tgctgaaaac
     9901 ccgtgaacgc cagaagggca aaaaccaata ccagaaactg ggcgagaagg gcgagtttct
     9961 tgaagttacc gaatataacg ctcacttgtg ggtgaacctg acggattatc tcgatactgg
    10021 tctgttcctc gatcaccgca tcgcccgtcg tatgctcggt cagatgagca aaggcaaaga
    10081 tttcctcaac ctgttctctt ataccggcag cgccaccgtg catgcgggat taggcggtgc
    10141 acgcagcacc accaccgtgg atatgtcgcg tacttatctg gaatgggcag aacgcaacct
    10201 gcgtctgaat ggcctgaccg ggcgtgcgca tcgcctgatt caggccgatt gcctggcgtg
    10261 gctgcgtgag gcaaatgaac agttcgatct gatctttatc gatccgccaa ccttctctaa
    10321 ctcaaaacga atggaagatg cgtttgatgt tcagcgcgat catctggcgc tgatgaaaga
    10381 tttgaaacgt ctgctgcgtg caggtgggac gatcatgttc tcgaacaaca aacgtggctt
    10441 ccgtatggat ctcgacggcc tggcaaaact gggactgaaa gcacaagaaa ttacgcaaaa
    10501 aacgctctcc caggatttcg cccgtaaccg ccagatccac aactgctggc tgattaccgc
    10561 agcctgaaag gaatagtaat gtcattaatc agtatgcatg gcgcatggct gtcgttcagc
    10621 gacgcgccgc ttctcgataa cgcagaactg catatcgaag ataacgaacg tgtttgtctg
    10681 gtgggccgca acggcgcagg caaatcgacg ttaatgaaaa tcctcaaccg tgaacaaggg
    10741 ctggatgacg gtcgcattat ttacgagcaa gatttgattg tagcgcgtct gcaacaggat
    10801 ccgccgcgta acgttgaggg tagcgtttat gatttcgttg ccgaaggcat tgaagaacaa
    10861 gcggaatatc tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag
    10921 aaaaatctca acgaactggc gaaggttcag gaacagctgg atcaccacaa cctgtggcag
    10981 ctggaaaacc gcatcaacga agtgctggcg caactggggt tagatcctaa cgttgcgctg
    11041 tcgtcgcttt ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct ggtgagtaat
    11101 ccgcgcgtgc tgttgcttga tgaaccgaca aaccacctgg atattgaaac catcgactgg
    11161 ctggaagggt ttttgaaaac tttcaacggg acgattattt tcatctccca cgaccgttcg
    11221 tttatccgca atatggcgac gcgcattgtt gatctcgatc gcggcaagct ggtgacctat
    11281 ccagggaatt acgaccagta cctgctggaa aaagaagaag ccctgcgcgt ggaagaatta
    11341 caaaatgccg agttcgatcg caaactggcg caggaagagg tgtggatccg ccaggggatc
    11401 aaagcacgcc gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcgat gcgtcgcgaa
    11461 cgtggtgaac gtcgcgaagt gatgggtacc gcaaagatgc aggtggaaga ggccagccgc
    11521 tccggtaaaa tcgttttcga aatggaagac gtttgctacc aggttaacgg taagcaactg
    11581 gtgaaagatt tttctgccca ggttctacgt ggcgacaaaa ttgccctgat tggtccgaat
    11641 gggtgcggca aaaccacgct gctaaaactg atgctcggtc agcttcaagc ggacagcggg
    11701 cgtattcacg ttggcaccaa actggaagtg gcttatttcg atcagcaccg cgcggaactg
    11761 gatcccgata aaacggtgat ggataacctt gccgaaggta agcaagaggt gatggttaac
    11821 ggcaagccac gccacgtatt gggctatttg caggactttc tgttccatcc gaaacgggcg
    11881 atgacgccgg tacgtgcgct ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg
    11941 ttcctcaaac caagcaactt attgattctt gacgaaccga ccaacgatct tgatgtcgaa
    12001 acgctggaac tgctggaaga actgatcgac agctatcagg gcacggtatt gctggttagc
    12061 cacgatcgtc agtttgtcga taacaccgtt acagaatgtt ggatcttcga aggcggcggt
    12121 aaaattggtc gttatgtcgg cggttatcat gatgcccgtg gtcagcaaga gcagtatgtg
    12181 gcgctcaaac agcctgcggt gaaaaaaacc gaagaagccg ccgcggcaaa agcagaaact
    12241 gtaaaacgca gcagtagcaa actaagctat aaattgcagc gcgaactgga gcagctaccg
    12301 caattgctcg aagatctgga ggcgaagctg gaagccctac agacgcaagt ggcggatgct
    12361 tccttcttca gtcagccgca tgagcagacg caaaaagtgc ttgctgatat ggctgctgca
    12421 gagcaggagc tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt
    12481 ggctgatcgc aaaatagtcg atttaaacag agacagctga caacggtaaa attgcctgat
    12541 gaactgggtt tatcaggcat gaaaaccaac actttgttag tcatctgtaa aatagcgcat
    12601 cattaaggag taccaatgtg cgaacatcat catgccgcga agcacatcct gtgctcgcag
    12661 tgtgacatgc tggtggcgtt accgcgcctt gagcatggtc agaaagcggc atgtccccgg
    12721 tgtggcacaa cgttaaccgt ggcgtgggat gcccctcggc agcgtcccac cgcctatgcg
    12781 ttggctgcac tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt
    12841 gcaggagtta ccagtgaaat tacattactg gaaattcccg gcgtgctttt ttctgaggac
    12901 tacgccagcc tcggcacctt tttcctgttg tttgtgcaac tggttcccgc gttttgtctg
    12961 ataaccattc tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa agagcaactg
    13021 gcacgggtgc tttttcaact caaaacctgg ggaatggcgg agattttcct cgctggtgtg
    13081 ctggtcagtt tcgttaaact gatggcttac ggcagcattg gggtaggcag cagctttctc
    13141 ccctggtgtt tattttgtgt cctgcaactg cgcgcttttc agtgcgttga tcgtcgctgg
    13201 ttatgggacg acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg
    13261 gggatacgtc aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa
    13321 cccgtgtgcc cgcgttgtag taccaaaggg tacgttcggc gtagaaacag cctgcagtgg
    13381 acactcgcgc tgcttgtaac gtccatcatg ctgtatcttc cggctaatat tttgcccatc
    13441 atggtgacgg atttattagg ctcgaagatg ccgtcgacga ttctcgctgg ggtcattctg
    13501 ttatggagcg aaggctctta tcccgtcgct gcggtgatct ttctggccag tattatggtg
    13561 ccaacgttaa agatgatcgc catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag
    13621 cgcgacagtg aaagaatgca tttgatttat gaagttgttg agtttgtagg ccgctggtcg
    13681 atgattgacg ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg
    13741 agtatttatc cggcaatggg tgcattaatg tttgctttag tcgtcataat gacaatgttt
    13801 tctgctatga cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag
    13861 gagtcctgac agtatggaat ctaataatgg ggaagccaaa atccagaaag tgaagaactg
    13921 gtctcccgtg tggatatttc ctatcgtcac ggcgctcatt ggggcctggg ttctttttta
    13981 tcattacagc catcagggac cggaagtgac cctgatcacc gcgaatgcgg aaggaattga
    14041 aggtggcaaa accaccatta aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac
    14101 actggctgat gatttgacgc acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa
    14161 attgctgcat aaagacaccg tcttttgggt ggtgaaaccg cagattggtc gcgaagggat
    14221 tagcggcctg ggaacgctgc tgtctggagt ttatatcgaa ctgcagccag gcgcgaaagg
    14281 cagcaaaatg gataaatacg atttgctgga ctcgccaccg ttggccccgc ctgatgcgaa
    14341 aggtatccgt gtgattctcg atagcaaaaa agccgggcag ctctcgccag gagatccggt
    14401 gctgttccgt ggctatcggg taggttcggt tgaaaccagc accttcgata cacaaaaacg
    14461 caatatcagc tatcaactgt tcatcaatgc accttatgac cgactggtga ccaacaatgt
    14521 tcgcttctgg aaagatagtg gcattgcggt tgatctgacg tcagcaggga tgcgtgtgga
    14581 gatgggctca ttgacaacgc tgctgagtgg cggtgtcagc tttgatgtgc cggaaggtct
    14641 ggatttaggg cagccagtgg caccgaaaac agctttcgtt ttgtatgatg atcagaagag
    14701 cattcaggat tcgttgtaca ccgatcacat tgattatctg atgttcttta aagattcggt
    14761 acgcggtctg caaccgggag ctccggtaga gttccggggt attcgcctgg gtaccgtaag
    14821 caaagtgcca ttctttgcgc cgaatatgcg tcagacattt aacgatgatt accgtattcc
    14881 ggtactgatt cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa atgcggatgt
    14941 tgttgagcac cttggcgaat tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa
    15001 cctggtcact ggtgcactgt atgttgatct cgatttctat ccaaatacgc ctgcaataac
    15061 cggtattcgt gaatttaatg gttatcagat tatcccgacc gttagcggcg gcctggcgca
    15121 aatccagcaa cgactgatgg aagcgttgga taagatcaac aaactgccat tgaatccgat
    15181 gattgaacag gcaaccagta cgctttctga aagtcagcgc acaatgaaaa acctgcaaac
    15241 gacgctggat agcatgaaca agatcctcgc tagccagtcg atgcagcagt tgccgacgga
    15301 tatgcagtca acgttgcgtg aattgaatcg cagcatgcag ggcttccagc ctggctccgc
    15361 agcctacaac aagatggtgg cggatatgca gcgccttgat caggtgttgc gagaactgca
    15421 accggtgctg aaaacgctca atgagaagag taacgcgctg gtatttgaag cgaaggacaa
    15481 aaaagatcca gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac gattgcagca
    15541 ctgtggctgg ccggatgcag ctccggcgaa attaataaaa actattacca gttacctgtg
    15601 gtgcagagcg gtacacaaag taccgccagc cagggcaatc gtctgttatg ggtagagcag
    15661 gtcactgttc ctgactatct ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag
    15721 tatgtgattg ccaacaacaa cttgtgggcc agcccgttgg atcaacagtt gcgcaacacc
    15781 ctggttgcca acctgagtac gcaactgccc ggctgggtgg ttgcctccca gcctctggga
    15841 agcgcccagg acacgctcaa tgttaccgta acggagttta acggtcgcta tgatggcaag
    15901 gtcattgtca gtggtgagtg gctgttgaac caccagggac aactgatcaa acgtccgttc
    15961 cgtctggaag gagtgcaaac tcaggatggt tacgatgaga tggttaaagt gctggccggt
    16021 gtctggagtc aggaagccgc ttctattgca caagagataa agcgtctacc ttaattataa
    16081 agatttgtaa atataaccgt ctccggtatg ttgcctgagg cggttttttt gtctctaacg
    16141 tgcggaaaaa tttgttcctc ttcacatttt ttgtacaacc gacatgcccg tgtagctcac
    16201 aaatatgaca gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc ggtattgctt
    16261 aactgtgatt gcacatttag taatcactgt tttcttttcc accagaaacc agtatgaggg
    16321 aaacgaggca tgaagagaca aaaacgagat cgcctggaac gggcacatca acgtggttat
    16381 caggccggca tcgccggaca ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg
    16441 tcacaatggc tgggaggctg gcgagaagcc atggcggaca gggtagtaat ggcctgattc
    16501 tgtctcttta aaaagaaacc tccgcattgc ggaggtttcg ccttttgata ctctgtctga
    16561 ttataatcag aaggcagacg tatcctggaa cagaccgact ttcaggtcgc tggcggtata
    16621 gatcagacga ccatcaacca gcacttcgcc atccgccagg cccataatca gacgacggtt
    16681 aacaatgcgt ttaaagtgaa tacggtaggt cacttttttc gctgtcggca gtacctgacc
    16741 agtgaatttc acttcgccaa cgcccagcgc gcggccttta ccttcgccgc ccagccagcc
    16801 gaggtagaac cctaccagct gccacattgc gtccaggccc aggcatcccg gcataaccgg
    16861 atcgccaata aagtggcatc cgaagaacca cagatccgga ttgatatcca gttctgcttc
    16921 aacataccct ttgtcgaagt taccacccgt ttcggtcatt ttgaccacac ggtccatcat
    16981 cagcatgttc ggtgctggca attgcgggcc tttagcgcca aacagttcac cgcgaccaga
    17041 ggcaagaagg tcttcttttg tataggattc gcgtttatct accatgttct ctgtaagcct
    17101 tattttattg aagcacgcag gatagctaac acgtgtacgc tgaacaagtc cgatcagttc
    17161 ggaataaacc agttcagcca acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc
    17221 gatgcttgcg cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc
    17281 cacaccagat ttaataataa cggcagtgcg tcagtcacat cgtctactgc ccagatggtg
    17341 aatttgcctt cttctaccgc tttcaccagt tcactgtgaa gacttaaatg gcgaacgtta
    17401 gctgtgggga taatgacacc ttgtttcccg gttaactcac gttgctggca aatagcaaag
    17461 aagccttcga ttttctcatt taaaccaccg accggctggg cgcgaccgaa ctgatcgact
    17521 gaacctgtga tagcgatact ctgattcacc ggcacatcgg cgagggcgct tatcagggcg
    17581 cagagttcag ccatcgaggc actatctcca tcaacttcac tgtatgactg ctcaaatgtc
    17641 agcgatgctg agaaggggat ctgttgctca agctgtagtt ccgacatcag gaacgcttgc
    17701 atgatcatca tccctttcgc atggatattg ccgccaagct ccgctttgcg ttcgatgtcg
    17761 gtgaattcac catcgccaat atgcacaacg cagctaatgc gagaaggttc gccaaaagcg
    17821 cgtggatgac ccggaaattc aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct
    17881 tcggtttcaa tcaggatttg ctcctgaagg atctcatcct gcatacgttc agcgaggaaa
    17941 ccttcgcgcc attcacgctg ctgcagcatt aagtttagct gctcgccgga gaaggtgtcg
    18001 ccatcacaca gggaggcgac ctctttacac tggcggagga tccactgcgg gctaagcgga
    18061 agtgtttctt gttcaccggt gtagcgtgct gcttcgcgga taagtatcgg ccaggcatcc
    18121 gctcccggtg caggtaagtg attatgtctg gcggtaaatg tcacccagcg acaccactgg
    18181 gttactgact ccgcatcgac aatctgcaga gtatcttcaa attcgctata aatagcctgc
    18241 tctgaaagct ctggctccat ctcctggaaa tcagccaatg attcgcgttc gcctaccaga
    18301 atgactttca gcttcaatgg catcgaaggc acagagacgg ggagagggcg cgactcatca
    18361 aacgcaaccc agtcaaaacg ctcgcggtta acgatatttt tcagccgcat ccacagcaga
    18421 ggttgcgcca gcagtgtacg caaagagatg atgagaatac cgccatttgc ctgatgcacc
    18481 agaccaggct gcagggtaat gtcgccatta aactggcgca ggcagccaaa gagttgctcc
    18541 gcttctaccc agtcggcagc gacaacttgc gttaaagtcg caaaattatc atctgcactc
    18601 actgcgtgac gtaagcggat ggagtggccg gaaacctcat agtgaccgcc caccagttgg
    18661 cctgcatcgc tttgtagcgt acgcgcggca ttggcaataa gattcagata ctcagactct
    18721 tccggggcct tcgccagcat aaaagaggag gatgctcgcg tatgcagcaa ctgctccagc
    18781 gcaaattgca gccgcggttg agtatcactg aataaaggat cgttttcgtc aatcaaatgt
    18841 ggctgagcaa atatttcctg atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt
    18901 ttcgtaatgg tcaaagttga tgttttttag tctgttgtca aagccgcgat tataccgtaa
    18961 ccggcactac agcacacgta gaaagcaccg acaatactcc tggcatgggc gttaaagctc
    19021 acaggatgga gattcttttc ttcactggcc taaaaagctg atattctgta aagagttaca
    19081 cggtaacatt gagatcgcta tgaaatatca acaacttgaa aatcttgaaa gcggttggaa
    19141 atggaagtat ctggttaaga agcatcgcga aggggagtta atcacccgtt acatagaagc
    19201 cagtgccgcc caggaagccg tcgatgtgtt gctctcgctg gaaaatgaac ccgtgctggt
    19261 aaatggctgg attgacaagc atatgaatcc ggaactggtc aatcgcatga agcaaaccat
    19321 tcgggcaaga cgaaagcgcc attttaatgc ggaacaccag catacgcgca aaaaatcgat
    19381 cgatctggaa tttattgtct ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac
    19441 gctttctgaa acgattgttc agctgattga agatgcggaa aacaaagaga aatacgcgaa
    19501 taaaatgtct tctctgaagc aggatctgca ggcattgctg ggtaaggaat aactgacgaa
    19561 agtcagttca atttactaaa ggcaaaaaaa accccgcagc agcggggttt ttctaccaga
    19621 cgagaactta agcctgcggc tgagttacaa cgtctttgat acctttaact tcgatctcta
    19681 cgcgacgatc cggagccagg cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg
    19741 tgttgccagt aaccgggttg gattcgccca taccacgtgc ggagatcttg tctgccggga
    19801 tacctttgga gatcaggtaa tcaacaacag actgagcacg gcgctcggac agaccctggt
    19861 tgtaagcgtc agaaccgatg cggtcggtgt aacccagaac aactacggaa ccgtctttcg
    19921 gatccaggtt gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca
    19981 gggttgcttt gttgaagttg aacagaacgt cagacttcag agtgaagtgc ttggtctgta
    20041 cttccggtgc cggagctgga gccggagcaa ctactggagc tgcttcgccc tgaccgaaac
    20101 ggtaggaaac acccaggctc agcatgccgt tgtccggacg agtgccgatg gtgtgtgcgt
    20161 caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt
    20221 actcaacacc gccagcgaag accggagaaa cgccggtgtc gtggttttta ccataaacgt
    20281 tggatttagt gtctgcacgc cataccatac cacccagacg agtgtagatg tctaggtcgt
    20341 cagtgattgg gtaacccagt ttagcggtca gttgaacgcc ctgagctttg tatgcaccgt
    20401 tttcaacgct gcctttgtac ggcatacgac ctaaccagtc gtaacccatt tcaaagccaa
    20461 catacgggtt aacctggtaa ccaccaaaag caccagcgcc cagttggttt tcatgggtcg
    20521 ggccattgtt gttgatgaaa ccagtgtcat ggtactggga ccagcccagt ttagcaccag
    20581 tgtaccaggt gttatctttc ggagcggcct gcgctacggt agcgaaacca gccagtgcca
    20641 ctgcaatcgc gatagctgtc tttttcattt tttgcgcctc gttatcatcc aaaatacgcc
    20701 atgaatatct ccaacgagat aacacggtta aatccttcac cgggggatct gctcaatatt
    20761 aactctaccg atatcttcgg cttatgccga gcacccctgg cgatgtaaag tctacaacgt
    20821 agttgaaaac ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc ttgtataagg
    20881 tatgtttaat cttttttgtc agcgacaatt tacagaagag aatcgcggaa accgcttcag
    20941 acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag
    21001 ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga
    21061 aagttgtctc gtggcgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt
    21121 accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgccaa
    21181 ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg actccacagt
    21241 gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg
    21301 aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg
    21361 accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg
    21421 atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt
    21481 acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt acataatcaa
    21541 tccagcccct gtgagttact gtatggatgt acagtacatt cagtgacaac aaagatcaac
    21601 cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac ataaattaat
    21661 cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta atgtgaatca
    21721 ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat aagcggatct
    21781 ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca ttgtttggca
    21841 gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt gagttgtatc
    21901 ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc tggctgacat
    21961 ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa agtctatggc
    22021 gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg ctgaaagaga
    22081 aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct tttcatctgg
    22141 aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata cttggggcaa
    22201 aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag attctgttta
    22261 tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca tgccgccagg
    22321 agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg gaacttgagt
    22381 aatctggcgt tgcagacgac caatctcagg cagtaatgca atcaataatc caacttgttg
    22441 tacgaccaga ggttctttgc tgtctgcccg tggttcaagt tgctgcatcc gctgtttcag
    22501 gctagctaat gcctcattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc
    22561 aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta actgctcccg
    22621 gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaaccgaaa
    22681 cgcggcttcg cgaatttgcg gggtaacgtt cggctcgctg gacatatttg ataccaccga
    22741 cgccagctca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt
    22801 atcacgcccc tgatggtatt gctccagtat ggcatcgaga taccgacagt tggcctctgt
    22861 ggcgcgttcg agcatgcgcg gcagattgcg aaactgccag tcaggccaga tgtagctcac
    22921 tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc
    22981 aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc
    23041 atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac
    23101 cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatgg cgatacctac
    23161 cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca
    23221 gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta tctgaatgat
    23281 ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc
    23341 cggcgtgaag tgacggctaa gacgcagcca gatatcactc aacccgtgcg ggctgtcatc
    23401 agcgagctca ttttcgtcat tattatgggg tagtgcctgg gcctgttctg attcaattgt
    23461 tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa
    23521 atcggcgggt gcgccgttat cgcgcatccg ctccagcgca gcatcaatat gcgtaaaagc
    23581 gcgctcaaaa tgcggatcat gttgataagg ctgacgcaac aaaatacagc gtgacagttg
    23641 ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg
    23701 gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat
    23761 atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc
    23821 acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gcagaccgtt
    23881 ggcgagagcc aaatcgtaca gcggtgcctg gctttgatct tcaatatcag gatcaaacat
    23941 gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg
    24001 cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc
    24061 accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat
    24121 agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc
    24181 gccgagcaaa atgaagccgc tggtagagaa cgttaagcca atcgcaaata gccagggcca
    24241 gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag
    24301 gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc
    24361 cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc
    24421 acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccagg tatagcgttt
    24481 gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt
    24541 ggcttcacgc gcagcttgcg ctgtttctac cgatactacg cgacgaccaa ccggccatag
    24601 tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca
    24661 ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca
    24721 gaaaatattc aataccgtac cgccagtatt cagcagcaca tttttgccag ccgggttcag
    24781 ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca
    24841 gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag
    24901 ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg
    24961 cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg
    25021 gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca
    25081 gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc
    25141 gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattt
    25201 ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat
    25261 ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac cgaatggggc gagacgcagc
    25321 gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg
    25381 cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat
    25441 ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct ttgtcggcgt
    25501 tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat
    25561 gtcaggcctg gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg
    25621 aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc
    25681 cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg gcaggtgcag
    25741 gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag
    25801 cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg
    25861 agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgctgg
    25921 cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg
    25981 ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga
    26041 aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag
    26101 aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct
    26161 gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg
    26221 aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct
    26281 ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga
    26341 ttgtgattct ggagaaaggt cgctttatca gcccgtggaa gcatattctg gttgatgaat
    26401 ttcaggatat ctcgccgcag cgggcagcgt tgttagcggc attacgcaag caaaacagtc
    26461 agacgacgtt gttcgctgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc
    26521 aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgaacgc tgtgatttag
    26581 acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga
    26641 acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagccgtca
    26701 cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt tatgccaaac
    26761 cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa
    26821 aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag
    26881 ggcaacaggc ggattacgtc atcatcgttg gcttgcagga gggaagtgat ggttttccgg
    26941 ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg
    27001 acgctgaaga acggcggtta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg
    27061 cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat ctggatgtgc
    27121 cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag
    27181 acggtccgcg agataacgct gataatcggg gatcagaata tcgaccgcgt cgttgaaatg
    27241 cggcgactgg attatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac
    27301 cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc
    27361 ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc
    27421 accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaattaa
    27481 gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc
    27541 cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg
    27601 cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac tttcctacag
    27661 attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa
    27721 aaatctcagg aagatggttg tttttccgcc cactgcagga aagtatttcg cgtttgtggg
    27781 tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga
    27841 atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat catcgtggca
    27901 aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa
    27961 tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg aattggcgtc
    28021 gcatcgccat ccaacaattc aaggtgcggc gcggcatcaa aatggttagc ttcccgctca
    28081 ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc
    28141 accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca
    28201 acgcgaaaca ctaactgatg cggtccatta tccagttcaa tgctgtccgc accgcgcagc
    28261 agagagctgg aaaccttctt gccgtcgagt accagaagat cgacatcggt tgaaagccgc
    28321 agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg
    28381 gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct
    28441 ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt
    28501 agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata
    28561 atgaaagaaa ccgatattgc aggtatttta acgtccactc acaccattgc gctggtgggg
    28621 gcaagcgaca aacccgatcg tcccagctat cgcgtgatga aatatctact cgaccagggg
    28681 tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg
    28741 tatggcacgc tggcagacgt tccggaaaaa gtcgatatgg tcgatgtttt ccgtaattca
    28801 gaagctgcgt ggggcgtggc gcaggaagct atcgccattg gcgcgaaaac cctctggatg
    28861 caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg gttaaatgtg
    28921 gtgatggatc gctgcccggc tattgagatc cctcgcctgg gcctggccaa ataaaaaatc
    28981 cccggaaggc aaaaaccttc cggggattcg ttcagggatc agttacgcag acgcggggcc
    29041 tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca
    29101 tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta
    29161 tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt caccgccagc
    29221 tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga
    29281 taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc
    29341 acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg
    29401 acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat caccggatga
    29461 tcggctgcct gacggaactg ctctataaat tgtacatcac ggccggcatc aattgcggca
    29521 tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg
    29581 agaataccgc cttcattcag caactgaatc gccagcatgt tgatatcttt atagccccga
    29641 cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg
    29701 tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca
    29761 aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc
    29821 gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg
    29881 gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg
    29941 gtagccaggc ggctatcacg ctggtccagg tagtagcccg ttttgtgtcc gtgctgaata
    30001 tccaccagca gtttcattcc gtgttcttca atcggcagca gggcaggtgg caactcgccg
    30061 gtgacggggc cctgggtcag ctccattcct tcttttttac gtaccgcgac gtcgctgcga
    30121 tcgtaaatcg aacattccgg gtacagcgtt tgcagggcac taattaatgc cgcgcgctga
    30181 tattctgccc cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta
    30241 atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt
    30301 tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg
    30361 tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa
    30421 taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg
    30481 ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt
    30541 aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc
    30601 gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta
    30661 aggataagga gagcagcaat gtcgaaagtc tgcataattg cctgggttta cgggcgagtt
    30721 cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg tttaaccggg
    30781 tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cttgcggtga agaagggcag
    30841 gtggaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg
    30901 gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata
    30961 cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg acctttcgga
    31021 aacagtcggt ataaatagcg gctgttacct ttttcttcgc caaatttatt cgccatcgct
    31081 tttaccagca tacgaatcgc cggagaagta ttgaattcca gatagaaatc acgcacaaaa
    31141 cgcaccactt cccagtgttc cggtgacagc gaaatccctt cgttctctgc aatcaccaca
    31201 gccagcggct cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc
    31261 tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa
    31321 caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg
    31381 cggctagcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac
    31441 aggctaaccg tggcacgaat atagttcgtc tcaccgccat gaatgatgtt gctggtttca
    31501 aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag atgcagagca
    31561 ggcagctgca ggaagatatt cgcaaccata ccaatcagca ccaccacaat acccgccatc
    31621 agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag
    31681 aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac
    31741 agataggtgt tcagaatagg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg
    31801 gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata
    31861 cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg
    31921 gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta
    31981 tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca
    32041 ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga
    32101 agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca
    32161 ttaagcaaat ataacgccct gagaatttcg acaggcaaaa gaaaaagggg ttagcattta
    32221 gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg
    32281 tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca
    32341 ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc
    32401 caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc
    32461 ttctctttta ctcgtttagc aaccggctaa acatccccac cgcccggcca aaagaaaaat
    32521 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    32581 ttcactacgc tttattaaca atactttctg gcgacgtgcg ccagtgcaga aggatgagct
    32641 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    32701 gaccataagc gcccggtgtc ctgccggtgt cgcaaggagg agagacgtgc gatatgaata
    32761 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    32821 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    32881 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgca
    32941 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    33001 ccctgatttc cctcgattac gacgatactt tgatggctgc cgccggaacc caggcggaag
    33061 aagtctttga agacatcatc acgcaataca atggcaaata tatcctcgca gtagaaggta
    33121 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgata
    33181 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    33241 ggggctgcgt gcaggccgcg cgacccaatc cgacgcaggc aacgcctatc gacaaagtca
    33301 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    33361 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggccgtc
    33421 cgctgatgtt ctatggtcag cgaatccacg ataaatgcta tcgccgcgcc cacttcgacg
    33481 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttac tgcctgtaca
    33541 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    33601 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    33661 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    33721 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcggctgtt ggtgtgcacg
    33781 cagtcgccag cgccgttgac cagcgcagac gtcataacca gcaacctaca gaaaccgaac
    33841 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    33901 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    33961 gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    34021 catgtttcgc gggctggaga tcatcctaca agggcgcgac ccgcgcgatg cgtgggcgtt
    34081 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    34141 cgaagatgct atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    34201 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    34261 gatcgatgtg ttagatgcgc tgaaagccga cccgcggaaa acctccgaac tggcgcaaag
    34321 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa actgcctgaa
    34381 aaaatttgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    34441 gtacaaactg ccgccagaag ctaacctgat gggctttgcc cactatctcg aagctctcga
    34501 tttccagcgt gaaattgtca aaatccacgc ggtctttggc ggtaaaaacc cgcatccaaa
    34561 ctggattgtc ggcgggatgc cttgcgccat caacattgac gaaagcggcg cggtcggggc
    34621 agtcaatatg gaacgcctga acctggtgca gtcaattatc acccgcacgg cggacttcat
    34681 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    34741 aatcggcact ggtctttctg ataaatgcgt tctcagctac ggcgcattcc cggatattgc
    34801 caacgacttt ggcgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    34861 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgacca
    34921 cgcctggtat cgatatccca acgatcaggt cgggcgtcat ccgttcgatg gcatcaccga
    34981 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    35041 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    35101 gccgctggcg cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    35161 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttaggccg
    35221 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    35281 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccacgg aaaaatggga
    35341 acctgcaacc tggccgacag agtgccgtgg tgtcggtttt accgaagcgc cgcgcggggc
    35401 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    35461 gaccacctgg aacgccagcc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    35521 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatcctgc gtactctgca
    35581 cagctttgac ccgtgcctcg cctgttcaac acacgtgctg ggcgacgacg gtagcgagct
    35641 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    35701 cgttgtcagc cactatgtct ttgaagcgcc agtgcgcatc tggcactggt tgacggtgtt
    35761 atgcatggcg gtgttgatgg tcaccggata ctttatcggc aagccgctac cttccgtcag
    35821 cggcgaggcg acgtatctgt tctatatggg ctacatcagg ttaattcact tcagcgccgg
    35881 gatggttttt accgtggttt tgctgatgcg gatctactgg gcttttgttg gcaatcgata
    35941 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    36001 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataatcc
    36061 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcactgg
    36121 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    36181 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    36241 gatgtggctg attggcgcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    36301 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    36361 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    36421 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    36481 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    36541 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    36601 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cttatctcag cgcgaagaaa
    36661 atgagcctgc atcagaacag tttctccgaa gtgttggcgc tggcggatat ccgcggacat
    36721 ctgccagcac atattgccct cgtcggtctg caacccgcaa tgctcgacga ctacggcggt
    36781 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaggcggc gctggcgcag
    36841 cttgctgcgt ggggaattgt gccgcaaccg gctaatgaat cgcgctgtct caattatgac
    36901 tgtttgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    36961 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    37021 gctggacgcc agtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    37081 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    37141 ttggcgaatt actgcgcgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    37201 agcagagcga agccatcggc gatcgctttg gcgtctttcg ctttcctgcc actttagtgt
    37261 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    37321 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    37381 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    37441 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ctggagcaat gtgctcacag
    37501 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccaaccgcc
    37561 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    37621 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtga
    37681 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    37741 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    37801 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    37861 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    37921 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcacgtct
    37981 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    38041 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    38101 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    38161 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    38221 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    38281 cgtagagttt caggataaag agggagatct accattatcg ggttattttt ctctcttcgc
    38341 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    38401 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    38461 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    38521 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    38581 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccgc
    38641 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    38701 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    38761 gttcaaatct ctctgcgttg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    38821 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    38881 ttaatccggt cagccaggtg aaatttgtgc acaccgtaat ggcgggctac gtgaccgggg
    38941 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    39001 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    39061 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    39121 tggaagggga gtggcaaacg gaacctgcac ctgcaccgtt ccatgtggtt gcctggccgg
    39181 aacaggatca agagcgtaac gcctttgccc tcaaaattcc cgcgctgcta gggatcctcg
    39241 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    39301 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatcg caaggcaatc
    39361 gtgagccgca tgtgttgcag gcattccggg gactggaagg tgacctgggc tacggcatgt
    39421 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    39481 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    39541 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    39601 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    39661 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    39721 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    39781 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    39841 atgcccgtct ggggccgagt gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    39901 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    39961 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtctgctgcc
    40021 gctggtggcg cgtaatgatg atgaacgccg gatagtgata aacagcgttg gtgcacactg
    40081 ggaaggcaac caggtctggt tgatcctcgc tggtggggca ttatttgccg cctggcccag
    40141 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcact
    40201 gttcttccgc ccgctggcct ttgattatcg cggaaaaatc gccgatgcac gctggcgtaa
    40261 aatgtgggac gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    40321 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    40381 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgcggat tgctcagcct
    40441 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    40501 tctgcgttca cagctggcca ccaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    40561 ggcgggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    40621 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    40681 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    40741 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttaatgg cgtcattgat
    40801 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    40861 cgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    40921 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    40981 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    41041 gtactaagga gcaggaacaa tgtggtattt actttggttc gtcggcattt tgttgatgtg
    41101 ttcgctctcc acccttgtgt tggtatggct ggacccgcgt ctgaaaagtt aacgaacgta
    41161 ggcctgatgc ggcgcattag catcgcatca ggcaatcaat aatgtcagat atgaaaagcg
    41221 gaaacatatc gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaaccc
    41281 cgcaatctgc attcgctcag agtgagccgg agctgaagct ggaaagtgtg gtgattgtca
    41341 gtcgtcatgg tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag
    41401 acgcatggcc aacctggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa
    41461 tcgcctatct cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa
    41521 agggctgccc gcagtctggt caggtcgcga ttattgctga tgtcgacgag cgtacccgta
    41581 aaacaggcga agccttcgcc gccgggctgg cacctgactg tgcaataacc gtacataccc
    41641 aggcagatac gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac
    41701 tggataacgc gaacgtgact gacgcgatcc tcagcagggc aggagggtca attgctgact
    41761 ttaccgggca tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat
    41821 caaacttgtg ccttaaacgt gagaaacagg acgaaagctg ttcattaacg caggcattac
    41881 catcggaact caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat
    41941 caatgctgac ggagatattt ctcctgcaac aagcacaggg aatgccggag ccggggtggg
    42001 gaaggatcac cgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt
    42061 atttgctaca acgcacgcca gaggttgccc gcagccgcgc caccccgtta ttagatttga
    42121 tcaagacagc gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca
    42181 cttcagtgct gtttatcgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg
    42241 agctcaactg gacgcttccc ggtcagccgg ataacacgcc gccaggtggt gaactggtgt
    42301 ttgaacgctg gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc
    42361 agactttaca gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg
    42421 tgaaactgac cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag
    42481 gttttacgca aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa
    42541 agagcattca gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg
    42601 tagaccggaa aaggcgttca cgccgcatcc ggccactttc agttttactc tttctcggag
    42661 taactataac cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca
    42721 ccattgagga tagcgccttt aatattgacg cctgcctgtt ccagacgctg cattgacaaa
    42781 ctcacctctt tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccaacagaa
    42841 cgccccacga ccgcggcatc actcaccgcc agcatcggcg gcgtatcgac aatcaccaga
    42901 tcgtaatggt cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat cagcagttca
    42961 gacgggttag gtggcacctg accgcgagta atcacatcaa agcctccttt gccaaaatgc
    43021 tggatcactt tgttgagctc atctttacct gccagatatt ccgacaagcc atgttcatta
    43081 ctcacggtaa acaggttatg cgaataacca cggcgtaagt cggcatcaat aaataacact
    43141 ttttgatcgg actgggcgat caccgctgcc agagttgaac tgacaaacgt tttaccactg
    43201 tctggcgtcg caccggtgat catcagaatg ttattctccg tctccatcat agcgaaatgc
    43261 agactggttc gtagcgcacg tacggcttcc acagcagaat ccgccgggtt atccaccgcc
    43321 aggaagggga tatttttagt acgatggcgc tgctgattag aaaataaatt tttcttacgc
    43381 agacgggtgc gtttatccag ccactcggac attgggatag tggcataaac gctgatgccg
    43441 tgctcttcca gttgttccgg ggcttctaca ccacgacgca acatcgcacg cgccagcacg
    43501 gcacccacag aaataaacag gccaagaata aaaccaagca ccacgttcaa cgcttttttc
    43561 ggtttcactg gctgcggctg agtgactgcc gggtcgataa tccgcacgtt accaatggca
    43621 ctggatttcg aaatactcaa ctcctgctgg cggttaagta attgcagata taccgcacgg
    43681 cccgcttcta cgtcacggct taaacgtaac acttcctgtt gagtggaagg cattgccgat
    43741 acccgcttat tcaggcgttt gcgttcttgt tccagcgtct ggcgtttttc cagcagcgca
    43801 cgataagttg ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaggtcagc
    43861 tcattgagtt gattatcaac gttcacaatc tgctcaagaa cggctttggc ttccaggtta
    43921 aggtcaaccg aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc
    43981 tcgctgcgca cttcaggtaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc
    44041 gcctggcgag cgatat
//
LOCUS       DACAPB010000010        44046 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.010, whole genome shotgun sequence.
ACCESSION   DACAPB010000010 DACAPB010000000
VERSION     DACAPB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.010"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(152..505)
                     /locus_tag="IT904_000509"
     CDS             complement(152..505)
                     /locus_tag="IT904_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAP3190343.1"
                     /translation="MIDKIKSNARDLRRNLTLQERKLWRYLRSRRFSDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            complement(573..1379)
                     /gene="sapF"
                     /locus_tag="IT904_000510"
     CDS             complement(573..1379)
                     /gene="sapF"
                     /locus_tag="IT904_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAP3190344.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(1381..2373)
                     /gene="sapD"
                     /locus_tag="IT904_000511"
     CDS             complement(1381..2373)
                     /gene="sapD"
                     /locus_tag="IT904_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAP3190345.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(2373..3263)
                     /gene="sapC"
                     /locus_tag="IT904_000512"
     CDS             complement(2373..3263)
                     /gene="sapC"
                     /locus_tag="IT904_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAP3190346.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(3250..4215)
                     /gene="sapB"
                     /locus_tag="IT904_000513"
     CDS             complement(3250..4215)
                     /gene="sapB"
                     /locus_tag="IT904_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAP3190347.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(4212..5855)
                     /gene="sapA"
                     /locus_tag="IT904_000514"
     CDS             complement(4212..5855)
                     /gene="sapA"
                     /locus_tag="IT904_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAP3190348.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(6167..6412)
                     /locus_tag="IT904_000515"
     CDS             complement(6167..6412)
                     /locus_tag="IT904_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAP3190349.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(6546..7931)
                     /gene="puuP"
                     /locus_tag="IT904_000516"
     CDS             complement(6546..7931)
                     /gene="puuP"
                     /locus_tag="IT904_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAP3190350.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(7921..8085)
                     /locus_tag="IT904_000517"
     CDS             complement(7921..8085)
                     /locus_tag="IT904_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190351.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(8234..9652)
                     /locus_tag="IT904_000518"
     CDS             complement(8234..9652)
                     /locus_tag="IT904_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAP3190352.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVASLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLLPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPSPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            9864..10628
                     /gene="puuD"
                     /locus_tag="IT904_000519"
     CDS             9864..10628
                     /gene="puuD"
                     /locus_tag="IT904_000519"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAP3190353.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYAPSRILFEGFITACQHHIAEKQRL"
     gene            10655..11212
                     /gene="puuR"
                     /locus_tag="IT904_000520"
     CDS             10655..11212
                     /gene="puuR"
                     /locus_tag="IT904_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAP3190354.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            11487..12974
                     /gene="puuC"
                     /locus_tag="IT904_000521"
     CDS             11487..12974
                     /gene="puuC"
                     /locus_tag="IT904_000521"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAP3190355.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            12976..14256
                     /locus_tag="IT904_000522"
     CDS             12976..14256
                     /locus_tag="IT904_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP3190356.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            14294..15565
                     /gene="puuE"
                     /locus_tag="IT904_000523"
     CDS             14294..15565
                     /gene="puuE"
                     /locus_tag="IT904_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAP3190357.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKMARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCKRANQLGQRLKN
                     TLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGA
                     YGNVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(15683..16660)
                     /gene="pspF"
                     /locus_tag="IT904_000524"
     CDS             complement(15683..16660)
                     /gene="pspF"
                     /locus_tag="IT904_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAP3190358.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            16827..17495
                     /gene="pspA"
                     /locus_tag="IT904_000525"
     CDS             16827..17495
                     /gene="pspA"
                     /locus_tag="IT904_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAP3190359.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            17549..17773
                     /gene="pspB"
                     /locus_tag="IT904_000526"
     CDS             17549..17773
                     /gene="pspB"
                     /locus_tag="IT904_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAP3190360.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            17773..18132
                     /gene="pspC"
                     /locus_tag="IT904_000527"
     CDS             17773..18132
                     /gene="pspC"
                     /locus_tag="IT904_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAP3190361.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            18141..18362
                     /gene="pspD"
                     /locus_tag="IT904_000528"
     CDS             18141..18362
                     /gene="pspD"
                     /locus_tag="IT904_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAP3190362.1"
                     /translation="MNTRWQQAGQKVKSGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            18437..18751
                     /gene="pspE"
                     /locus_tag="IT904_000529"
     CDS             18437..18751
                     /gene="pspE"
                     /locus_tag="IT904_000529"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAP3190363.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            18963..20642
                     /locus_tag="IT904_000530"
     CDS             18963..20642
                     /locus_tag="IT904_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP3190364.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINLSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            20656..21948
                     /locus_tag="IT904_000531"
     CDS             20656..21948
                     /locus_tag="IT904_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3190365.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            21969..22850
                     /locus_tag="IT904_000532"
     CDS             21969..22850
                     /locus_tag="IT904_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP3190366.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPDFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            22837..23679
                     /locus_tag="IT904_000533"
     CDS             22837..23679
                     /locus_tag="IT904_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP3190367.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            23710..24762
                     /locus_tag="IT904_000534"
     CDS             23710..24762
                     /locus_tag="IT904_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP3190368.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            24781..25569
                     /locus_tag="IT904_000535"
     CDS             24781..25569
                     /locus_tag="IT904_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP3190369.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            25579..26634
                     /locus_tag="IT904_000536"
     CDS             25579..26634
                     /locus_tag="IT904_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP3190370.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            26631..28898
                     /gene="ycjT"
                     /locus_tag="IT904_000537"
     CDS             26631..28898
                     /gene="ycjT"
                     /locus_tag="IT904_000537"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAP3190371.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETMTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAYTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLITVAEESVFCLGDF
                     ILPFNGTATKHQEDE"
     gene            28895..29554
                     /gene="pgmB"
                     /locus_tag="IT904_000538"
     CDS             28895..29554
                     /gene="pgmB"
                     /locus_tag="IT904_000538"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP3190372.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            29568..30650
                     /locus_tag="IT904_000539"
     CDS             29568..30650
                     /locus_tag="IT904_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3190373.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVSGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            30695..31600
                     /gene="ompG"
                     /locus_tag="IT904_000540"
     CDS             30695..31600
                     /gene="ompG"
                     /locus_tag="IT904_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAP3190374.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(31711..32709)
                     /locus_tag="IT904_000541"
     CDS             complement(31711..32709)
                     /locus_tag="IT904_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP3190375.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALKAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            32865..34262
                     /locus_tag="IT904_000542"
     CDS             32865..34262
                     /locus_tag="IT904_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAP3190376.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHARARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKEQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            34259..35320
                     /locus_tag="IT904_000543"
     CDS             34259..35320
                     /locus_tag="IT904_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAP3190377.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            35468..37009
                     /gene="tyrR"
                     /locus_tag="IT904_000544"
     CDS             35468..37009
                     /gene="tyrR"
                     /locus_tag="IT904_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAP3190378.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(37053..37559)
                     /gene="tpx"
                     /locus_tag="IT904_000545"
     CDS             complement(37053..37559)
                     /gene="tpx"
                     /locus_tag="IT904_000545"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAP3190379.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            37678..38643
                     /gene="ycjG"
                     /locus_tag="IT904_000546"
     CDS             37678..38643
                     /gene="ycjG"
                     /locus_tag="IT904_000546"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAP3190380.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARR
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(38618..39346)
                     /gene="mpaA"
                     /locus_tag="IT904_000547"
     CDS             complement(38618..39346)
                     /gene="mpaA"
                     /locus_tag="IT904_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAP3190381.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(39473..39610)
                     /locus_tag="IT904_000548"
                     /pseudo
     CDS             complement(39473..39610)
                     /locus_tag="IT904_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            complement(39616..39681)
                     /locus_tag="IT904_000549"
     CDS             complement(39616..39681)
                     /locus_tag="IT904_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190382.1"
                     /translation="MKHIQIRNSDMAWHIAANNLG"
     gene            complement(39681..40601)
                     /locus_tag="IT904_000550"
     CDS             complement(39681..40601)
                     /locus_tag="IT904_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP3190383.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            40739..41638
                     /locus_tag="IT904_000551"
     CDS             40739..41638
                     /locus_tag="IT904_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3190384.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            41975..43588
                     /locus_tag="IT904_000552"
     CDS             41975..43588
                     /locus_tag="IT904_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3190385.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(43639..>44046)
                     /gene="ynaI"
                     /locus_tag="IT904_000553"
     CDS             complement(43639..>44046)
                     /gene="ynaI"
                     /locus_tag="IT904_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAP3190386.1"
                     /translation="FDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVG
                     VIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVY
                     LKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT        10889 a        10994 c        11746 g        10417 t
ORIGIN      
        1 tattgttacc ccagcaacaa acaggctcat acagccccta accctttcat ggcgatggct
       61 gggacggttc agaccttgcc gaatattctc cagcaccgtc tccatgtttt caccacaaca
      121 gctattcggc tcggtctgcc ccctcgctct ttcagggaga gggtgaccgg cggttcagtt
      181 cctgcagaat attctccagc accgtctcct cattacaatc aatctcgtta ttccagaaac
      241 gcagcacggt ccagccctgc gactcaagcc agctagtgcg cctggaatca taggcaactg
      301 ctaaatcatg ctgcccacca tccagctcaa cgactacacg cgccgagcag caagcaaaat
      361 cgagaatgta gctccccact ggatgttgac ggcgaaattt gaaatcacta aaacgtcggc
      421 tgcgaagata tcgccagagc tttcgttcct gcaaagtgag attgcgtctt aaatcacgcg
      481 catttgattt aattttatct atcacctcat tctgacaaga tttaatcttt tgtcagaatg
      541 aggtgaataa tctggaagga ggattcagaa aattagcgat ctttacgcca cgcatccgcc
      601 gtcaatgcct cgccaaaatg accggcaatc agccgtttgg tgagttcatg cagcggcgat
      661 gccagcacat ccgcggtgct gcctcgctcg acaacctcgc cctgatgcat caccagcacc
      721 tggtcgctaa tgtgcttcat cattccgata tgctgggtaa cataaatata cgaaatgccc
      781 tgtttttcct gtaattccag catcagatta atcaactgcg aacgcatcga catatccagt
      841 gaagcgaggg cttcatcggc aataatgact tttgggcgca atatcagtgc gcgcgccaga
      901 cccagacgct gtttttgccc gggtgccaac atatgcggat agtaactgac gtgatccggt
      961 agcagcccaa ccatacgcat cgtttcaata atctgtttgc gacgctgttc cggttccagg
     1021 tcggtgttca gacgcaatgg aaaatccaga atttgcgaga tacgttgacg gggattcaac
     1081 gaggtcgaag gatcctgaaa aatcatgcga atacgctgac tgcggaagga ataatcgcca
     1141 aaatgcagcg gatgatcgtc aatcaataac tcgccgctgg taggctctat cattcccgcc
     1201 agcatttttg ccagcgtgga tttacccgaa ccattctcgc caataatcgc cagtgtctgg
     1261 ccttcacgta gcgtaaagct caagggtttt accgcttcta cggtctgacg acgaaaccag
     1321 ccggtccggt agcggaacgt tttacttaaa ttacgcactt caagcagcgt ttcgatcatc
     1381 tcactctttc tccatgttca gcgggaaatg acaggcatag agatgatttt tcgcccccgt
     1441 caaacgtggc gtcacaatgc attctcgttg tgcatacggg caacgtggcc ccagacgaca
     1501 accaatcggt aactgttcca gcagtgggat agcgccgggt agcgtattga ggcgactttt
     1561 atgcggcatc gcgctgccga agtctggtat cgcgcggatc agcgcctggg tataaggatg
     1621 atgtggcatc gtcaccaact ccttactcgg cgcggtttcc actgtttgac cgcagtaaag
     1681 cacgttaatt ttatccgccc attggctaag catttgtaag tcatggctga taagcaaaat
     1741 agtggtattg ctgttttggt tgagacgcgt cagcaggcga aagatttgcg cctgggttgt
     1801 tggctccatt gagttggtcg gttcgtcagc aatcagcaga cgcggttgat tcgccagtgc
     1861 aatggctatc atcactttct gacattcacc ttcggtcaac tcatagggaa aactgcgcat
     1921 cgcatctttg tgatctttaa tccccacgcg gtgcagcagt tcaatggcac ggcgtttgcg
     1981 ccagccaaaa cgctgccacc aacggccttt ataggtccag gctgggatgt tttgcatcaa
     2041 ctggcgaccc acacgttctg aagggtcaag acacgactgc ggttcctgga aaatcatcga
     2101 cacgttatgg ccaaccagtt tgcgccgttc gcgtgcggag agacgcagca aatcgatatc
     2161 atcaaaacgc atacggtcag cagtaacacg ccagttatct ttattcaccc cacaaatcgc
     2221 tttcgcaatc aaactcttgc cggaaccgga ttcaccaaca agaccgcgga tttcaccttc
     2281 ggttaaggtc atacttaccc ggtcgacggc tttaacccac tcatcaccgg ttttaaattc
     2341 aatggtcagg ttacgaatat cgagtaatgg cattattcca cccccgcaat aatcgcacga
     2401 cgaacgccat cgccaaggag gttaaccaac aacacgctaa tcataattgc cgcacctggc
     2461 agcatgacag tccacggggc gacataaatc agttccagcg catcaccgag catcgctccc
     2521 cattcaggcg aggggagttg tgcgccgaga tcgagaaagc ccagcgcggc gatatcgaga
     2581 attgccatcg acagtgcgcg ggtgatctcg gtgaccagcc cggcggtgat gtttggcatc
     2641 acagcaaacc agagaatatt cagcgttgat gcgccatcca gacgggcggc gataacgtac
     2701 tctttttcca gttcgtcatg caccatgctg taaatcgaac gtaccatacg cggcagcaat
     2761 gccagccaga cagcaaacat agcgtgggac aaactcggtc cggcaaacgc tacaacgata
     2821 attgccaaca gcagcgaagg gatcgccagt aaggtatcca gaatatggtt aagcaccgct
     2881 gagcggaggc cgtgtgtcgc cccggcgaag gtgcccagta ccaggccaca gatcgtcgcg
     2941 gcaagcgtca ccacaaatgc gccacccacg gttggcgcag ctccgctcag taaacggctt
     3001 aaaacatcgc gtccgaggtc gtcagtcccc aggaagaaag aaacttcgcc atagcgcgac
     3061 catgacggcg gcaataattg ataaccgaga aattgctggt cgatgccgta aggcgcaaac
     3121 cagccgccaa aaatacacag tacagccagc cccgcgcagc cgtacaggcc gaccatcgca
     3181 gaggcgtcac tataaaattt gcgccaggcg gtacgcagcg tgcccggcgg gcgcttttcg
     3241 ctgtatacgc tatcgtaagg cataccattc cttatgtttc agagggttag ccatggcacc
     3301 caaaatatca gaaatcacgt taacaataat gaccagtgag ccacacacca tcactccggc
     3361 ggaaatggct gcgtaatcct gctggcggat ggcgttaatt aaccagcgcc ctaaacccgg
     3421 ccagctaaaa accatttcgg tgatcatcgc cagcgtcagc atggtggaaa actgtaagcc
     3481 caggcgagga ataaccggcg gtaacgcgtt atgcagaacg tggcgacgca aaatagtaaa
     3541 gcgcgacaaa ccgcgggtcg ccgccgcttt tacatagttc tggtcataca cttcgatagt
     3601 gctgatacgc atcaggcgga tcacttcagt tgttggcgca accgacaggg tgatcacggg
     3661 caatatcata tggcggattg cgctcatgat catttcatcc cgccatggtg agtccgaaag
     3721 ccaggcatca atcaacgcaa aaccggtaat cggtttcact tcgtagagca gatcgaaacg
     3781 ccctgaaacg ggcaaccaac ctagcgtgag tgaacaaaac agcgtcaaca gaagcgccag
     3841 ccagaacacc gggattgaaa agcccagtaa ggcgatggcg ttgatcaagt tatcctgcca
     3901 cttatggcgc gtaatcccgg caatcatccc cacggggatg ccaacaatca gcgcaaaacc
     3961 gaaggcgagg atgcacagtt ccatcgtcgc cgggaagacc tcttttaact gttcggcaat
     4021 cggttgaccg ttaatgctgg aaacaccaaa atcccagtgg atcaggccgt taaaccagaa
     4081 cacccaggca ttccacagtg acgcgccttg taacggcgca tgaggggtga aatagctcaa
     4141 gctgaagcca acgaaggtca gcaggaacaa ggtgacaatc aataacaaaa tgcggcgtaa
     4201 ggtgaagata atcatggttt tttcacctca tcctgtttct cgcgatacac cccagcaaag
     4261 gaggcgttac caaacgggct aagtaccaga cctttgatat cgtaccgata ggcctgcaaa
     4321 cgcaatgacg acgccagcgg caaaatgggc aattcctgcg ccagaatact ctgcgcttcg
     4381 tcataggctt caatacgcgc cgccagctgc tgcgaggaga gcgccttacg caacacgctg
     4441 tcgaatttcg gatcgcacca gtgggcgagg ttggtctgtg aatgaattgc cgcgcagctc
     4501 agtaaaggac ggaagaaact gtccgggtcg ttactgtccg tcgcccaacc ggataacgtc
     4561 agatcatggc tcatatccat caaccgcgcc tcctgaaagc gaccttctac cggcacaatc
     4621 accactttta cgccaacctg cgccatatcc gcctgaatca gttcggcagt tttcagtgga
     4681 ctggggttcc acgcctgcga acgtgtgggc acccacagtt tcagcgttaa attttccagc
     4741 cccaacgact tcaactgttc gcgcgatttc gccggattgt attcagtaat tttagcctcg
     4801 ttgtcatagg cccacgaggc gcgcggtaaa atagaggccg ccgtttcagc cgtaccataa
     4861 tagatggatt gcatcaggcg ctggttatta atcgccagtg ccagcgcatg gcggacagcg
     4921 ggattattta gcggcggttt ggcggtgtta aatgccagat aggcgacgtt catcccagga
     4981 cgcagcgtta aacgcaagcg cgggtcgtca cgcaaaatgg atagctggct ggcagcaggc
     5041 caggccagaa cgtcgcattc cccggtcagg agtttcgaca gacgtccggt gccgccggag
     5101 cctaaatcca ccactacctg cggcattaac ggtttaccgc gccagaagtc atcatgacgt
     5161 tgtaggcgaa taaattgccc ggcgcggtat tccgacaact gatacggacc ggtgccgacc
     5221 ggttgacggt cgagttgctc ctggcgatct tctttctcta acttccgggc atattctgcc
     5281 gacatgaccg aagcataatg ggttgcgagg tgccacaaaa aagaagcatc cggctgggcg
     5341 agtcggaact caacggtatg attatccagt ttgcggacgc ttttcacgtt atcggcaaat
     5401 tgcaggctgt cgaagtaggg gaagttgctg ccgttgacgt tatgccacgg gttgttgcgg
     5461 tcaaaaattc gctggaaggt aaacaccaca tcgtcggcat tcattttacg agtgggagta
     5521 aaccagtcgg ttttttgaaa cggaacatcg cgacgcaggt ggaagcgata ggtcgcgccg
     5581 ttgtcgagta cttcccagct ttcggcaagt tccggcatca ggcgataggt atagggatcg
     5641 acatccagca gtcgatcata aaactgggcg gcaagggtat cgacaattaa cccactgctc
     5701 gctttggatg ggttaaaggt gttgacttgc ccgctgacgc aatagacaaa accgctgtcg
     5761 cggatatcag catgcggggg agattcaggc gcggcgattg cctgaccact cacaagtcca
     5821 gcaatcacca aaagagacga taatacctgg cgcataatat taagggattt tatgtaaaga
     5881 ggctatctta ctaatattta atgacatttg ccattaccgt ttgtgttcag gggtcgtaat
     5941 gagggccgtg tggttggtcg ttgaacagat ggcgtcgcca tctgttcaac attcgtaccc
     6001 gtgatttctc tataactata actcacagaa caacttagcg aggagcagag cggtacgatc
     6061 gtgagaaacc cattaaagca cgctgcaatg cgtgtcattt taaccggatg cagagtgaac
     6121 gctttatccg gacaacgata ctgaccgatc gtctgcaatt aaataattac tcattacccc
     6181 attgattcaa aaactctgcg atctcatcaa tgcgtacggg attaatcccc gcttcagcag
     6241 ccatttcatg ttgggcttct tcgctgatct cttcattgtt catcaaacgg gtgagcagta
     6301 actggaagta gtgggccagc ggtgtgcgtt cagcgtctgc gacaggctct gcgcattcgg
     6361 tcgcgtactc atcggcaata tcaaaatatt ttagcgggat atcgtggttc attatttgcc
     6421 cctggggtta atgcgctaag cataagggcg gatgatagca ctcgtgatcc gggtcataca
     6481 tctttttata gttaatggcg cggcgcatta ccctcaggca ggataatgcg gcgcgcatcc
     6541 gactattacg tttcgctcac tggcgttctg tcaccttcgt acagcggcac tttgcgatag
     6601 cggcgaatca gataccacaa atatgcgccg cctaacgaag cccataccag acccagcgtc
     6661 agcgaggtgg cttcgaggtt aatccacagc acgcccaccg tcagcgcgcc aaccagcggc
     6721 attaataaat agtggaagtg ttccttccag cttttattca ttcctttgcg ccgccagaag
     6781 tggttgaaca ccgacaggtt aacgaaggta aacgctacca acgcgccgaa gttaattaac
     6841 gctgtggcgg taactaaatc gaagaacagc gccgacatcg cgacaatccc gaccataatg
     6901 acgttcagtg ccggagtccg ccatttcgga tgcacatagc caaacacgcg ctccggaaac
     6961 acattgtcgc gccccatcac atacagcaga cgtgatacgc tggcatgtga ggccaggcca
     7021 gacgctaacg tgttcacaaa cgtggtgcaa aggaaaatcg actggaacag cttaccgccg
     7081 acgtagagtg caatttcagg cagtgcggcg tccgggtctt taaagcggct gatatcgggg
     7141 aagaacagct gcataaagaa cgacgccgcg ataaagataa cgccaccata gaccgccgtc
     7201 aggaagatgg ctttcgggat cacgcgtgcg gcgtctggtg tctcttccga aagtgtggtc
     7261 actgcatcga aaccaaggaa agagaagcag acgatcgtcg ccccggtaat aatcggaatc
     7321 agatgcgcgt tctcgctgat aaacggctgt aacgaccaga ccgtgccaac gccttctcct
     7381 ttatgcagtc cctgaaccac caggaagata aacaccacca tgatggagat ttgcaccaac
     7441 acaaacaggg tattgaagtt agcgaccagg ttgacgctct tcagattcgc ggcggttaaa
     7501 atggcgacga aggttaccac ccacacccac ggcggcactt ccgggaagag ggcggagaga
     7561 tagattttcg ccaacaaaac gttgatcatc ggcaaaaaga gataatccag cagtgatgac
     7621 cagccgacca taaatccgac gtgcgggtta atcgactttt gcgcgtaggt ataggccgaa
     7681 ccggcctccg gaaactggcg aaccagtttg ccgtagctga tagcggtaaa cagcacgccc
     7741 gccagcgcca gcaaatagga cgccggaacg tgaccgtcgc taatgcctga cacaatgcca
     7801 aaagtatcga acacagtcat cggcgtgaga taggccagac ccatcattac cacctgccac
     7861 aatttcagtg attttcgcag acgggttttg ccgggttgcg cagcaatatt cagtggtgaa
     7921 ttaatagcca taattgctgc ctcccctgcg taataaggtt tgcggtcgtg tcgacctgaa
     7981 atcaaacctg tttagcaagg gaagcaaagg gggcggagtc cagtcagtcg caggaggggc
     8041 gcatacgcag cctccctcgc catgcgggga tttaatcatc ggcatcatct catttcctcg
     8101 tcacagtctt aatcgttaac ggaagcccgt tgccgcctgc gctccataac gcgacatccg
     8161 gaaaatgtct ggattaccag caaatgtcag gcctggctcc gctcaggccg atgaaacaac
     8221 cccgcaaggg gtattacgcg tttttcaaca tccactcaat ttctgtttct gtgatgaggc
     8281 gctcaaactg caacagctca tcatttttac aggcgtgata gacatggcaa aaccgttcgc
     8341 ctaaatagcg gcgcaggtga tcattctcga taaactcacc cagggcatcg ctctggcgaa
     8401 tcggggaggg taagccatcc tgttccagcc cgttgccttc gacttcttcc tgcaacggca
     8461 gctcgttatc aagaccgtgc aaaataccgg caaaaatcgc tgccatcacc agatatgggt
     8521 tggcatcggc acccgccacg cgatattcca cgcggtgatt atgacgatcg ccgcatggaa
     8581 tacgcagggc gacggtgcgg ttgttatggc cccacgacgc ctgcgtcggt acatacatcc
     8641 ccggctggaa gcggcgatac gagttcacgt ttggtgccag caacgccatt gatgacggca
     8701 acagatcgat cattccggcg agcatttttt tcagcagcgg cgaatcttcg ccttccgcgt
     8761 cagaaagcac gttctcgcca cgattatttt gcatactgat atggatatgc atcccgctgc
     8821 ccgcgtgctc ttcatacggc ttcgccataa aagtggcgtg catcttatgc ttttctgcca
     8881 tcagacgcac caggcgtttt agcgccagcg catcatcgca ggcttccagc acgttatcgg
     8941 tatggtaaag gttgatttca aactgacccg gcgaggcttc ggcgaccgca ccgtcggcgg
     9001 gaattaactg taactgcgcc agttcatcaa tatcattgag cacgtcggcg aagtggttga
     9061 ggttatcaac ggagtaaacc tggctttgtg tattgcggtc atcggtgccg ggcgcgcagg
     9121 gcggttgcag atacccttca gcgtcgcgct gacgatccag taaatagaac tccagctcta
     9181 ccgctacaac cgggaacaat ccgcgctggc gtagctgctg ccacaggcgg ttaagaacgt
     9241 tccgcggctc aacgtcaaag ggagcgccat cttcatcgac catggtcagg agcatctgac
     9301 cgataaactc tggatcggcg gcagaagggg ttaaggaacc gagaacagga acacaagtac
     9361 gatccggctc gcccatttcc tgacccagac ccgcttcttc tactacgttg cccagaatat
     9421 ccattgcaaa caccgaggcc gggaaataac accctttctc caacttcttc aggcttgcaa
     9481 cagggatacg ttttccacgg aagcaaccat tcaaatcagt aagtaaaaca tcaacatatt
     9541 gcgtattcgg gtagcgctcc aggtagcgtt tcacttcctg cgtaaaggcg ctaccccgcc
     9601 tctcttctga ctgctgaaca aagttctcta cttcaacgat attggtttcc atgattcttc
     9661 gcctttggtt tgttttccgc tcgttatcaa agcgtaaaat ataatgacca ccattcgaat
     9721 ctgtatgcaa actaaatgtt tgtcaaatgt taaattgagt ttgcaaaaat gaaagcccac
     9781 tgctagattg aaaaaatatt gaacataaag gtcatttaaa gcgcagtaat ggcgataatt
     9841 tagtccactt tgtgagattg agcatggaaa atataatgaa caatccggtt atcggtgtcg
     9901 taatgtgcag gaacaggctt aagggtcatg cgacccagac tctgcaagaa aagtacctga
     9961 atgccatcat ccacgcaggc ggcttgccaa ttgcgctacc acatgcgctg gcggaaccgt
    10021 cgttacttga acaacttttg ccgaaactcg atggcattta tcttcctggt agtcccagca
    10081 atgtgcagcc gcacctatat ggtgaaaacg gcgatgagcc tgacgccgat cccgggcgtg
    10141 atcttctgag catggcgata attaatgccg cactcgaaag gcgcatcccc attttcgcca
    10201 tctgccgggg tttacaagaa ctggtggtgg caaccggtgg ttcgttgcat cgcaagctgt
    10261 gcgaacagcc tgaattgctg gaacatcggg aagatcccga actgccggtg gaacagcaat
    10321 atgcaccgtc gcatgaagtt caggttgaag aggggggatt actgtctgcg ttgttacctg
    10381 aatgtagcaa cttttgggta aactctctac atggacaagg ggcgaaggtc gttagcccac
    10441 ggttgcgtgt tgaagctcgc tcgccggatg gcttagttga ggcggttagc gtcatcaatc
    10501 atccttttgc gctgggcgta cagtggcacc cggaatggaa cagtagcgag tacgcgcctt
    10561 cgcgtatatt gttcgagggc tttatcaccg cttgtcagca ccatatcgct gaaaaacagc
    10621 gactctgacc actacagttt aaggaaatgc aaatatgagt gatgagggac tggcgccagg
    10681 aaaacgcttg tcggaaatcc gccagcagca ggggctttca caacgtcgtg ccgccgaact
    10741 ctccgggctg actcacagtg ctatcagtac gatagaacaa gataaagtca gccctgccat
    10801 cagtacgctg caaaagctgc tgaaggtgta tggtctgtca ctctcggaat tcttttccga
    10861 gccggaaaaa cctgatgagc cgcaggtcgt cattaatcag gacgacttaa ttgagatggg
    10921 tagtcagggt gtgtcaatga agctggttca taacggtaac ccgaatcgca cgctggcgat
    10981 gatctttgaa acgtaccagc cgggcacaac cactggggaa agaattaagc atcagggtga
    11041 ggaaataggc actgtactgg aaggtgaaat tgttctgacg attaatggtc aggattacca
    11101 cctcgtcgcg gggcaaagtt atgccattaa taccggcatc ccgcacagtt tcagtaatac
    11161 gtcggcaggt atttgccgaa ttatcagcgc ccatacgccc accacgtttt aatctttttg
    11221 ttctgtaagc cgggtaagcg caatgcgccc ggcaatctat acacaaaatc attcaagttg
    11281 catcaaggcg gcaagtgagc gaatcccgat gagcttactc aggtaagtga ttcgggggag
    11341 cgaacgcagc caaggcagag gcggcttgaa ggatgaagtg tatataaaga atgtcgccag
    11401 aaataaacgg gcatacggcc cggggatctc tgcgccctga cgttcacaaa ctgcatatat
    11461 ctgatagacg tgaaacagga gtcataatga attttcatca tctggcttac tggcaggata
    11521 aagcgttaag tctcgccatt gaaaaccgct tatttattaa cggtgaatat actgctgcgg
    11581 cggaaaatga aacctttgaa accgttgatc cggtcaccca ggcaccgctg gcgaaaattg
    11641 cccgcggcaa gagcgtcgat atcgaccgtg cgatgagcgc agcacgcggc gtatttgaac
    11701 gcggcgactg gtcactctct tctccggcta aacgtaaagc ggtactgaat aaactcgccg
    11761 atttaatgga agcccacgcc gaagagctgg cactgctgga aactctcgac accggcaaac
    11821 cgattcgtca cagtctgcgt gatgatattc ccggcgcggc gcgcgccatt cgctggtacg
    11881 ccgaagcgat cgacaaagtg tatggcgaag tggcgaccac cagtagccat gagctggcga
    11941 tgatcgtgcg tgaaccggtc ggcgtgattg ccgccatcgt gccgtggaac ttcccgctgt
    12001 tgctgacttg ctggaaactc ggcccggcgc tggcggcggg aaacagcgtg attctaaaac
    12061 cgtctgaaaa atcaccgctc agtgcgattc gtctcgcggg gctggcgaaa gaagcaggct
    12121 tgccggatgg tgtgttgaac gtggtgacgg gttttggtca tgaagccggg caggcgctgt
    12181 cgcgtcataa cgatatcgac gccattgcct ttaccggttc aacccgtacc gggaaacagc
    12241 tgctgaaaga tgcgggcgac agcaacatga aacgcgtctg gctggaagcg ggcggcaaaa
    12301 gcgccaacat cgttttcgct gactgcccgg atttgcaaca ggcggcaagc gccaccgcag
    12361 caggcatttt ctacaaccag ggacaggtgt gcatcgccgg aacgcgcctg ttgctggaag
    12421 agagcatcgc cgatgaattc ttagccctgt taaaacagca ggcgcaaaac tggcagccgg
    12481 gccatccact tgatcccgca accaccatgg gcaccttaat cgactgcgcc cacgccgact
    12541 cggtccatag ctttattcgg gaaggcgaaa gcaaagggca actgttgttg gatggccgta
    12601 acgccgggct ggctgccgcc atcggcccga ccatctttgt ggatgtggac ccgaatgcgt
    12661 ccttaagtcg cgaagagatt ttcggtccgg tgctggtggt cacgcgtttc acatcagaag
    12721 aacaggcgct acagcttgcc aacgacagcc agtacggcct tggcgcggcg gtatggacgc
    12781 gcgacctctc ccgcgcgcac cgcatgagcc gacgcctgaa agccggttcc gtcttcgtca
    12841 ataactacaa cgacggcgat atgaccgtgc cgtttggcgg ctataagcag agcggcaacg
    12901 gtcgcgacaa atccctgcat gcccttgaaa aattcactga actgaaaacc atctggataa
    12961 gcctggaggc ctgaaatgac cgaacatacc agcagttact acgccgccag tgcgaataaa
    13021 tatgcaccat tcgacacact gaatgagtca atcacctgcg acgtttgcgt ggttggcggc
    13081 ggctataccg ggctctcctc cgcgctgcat ctggcggaag cgggctttga cgtagtggtt
    13141 ctcgaagcct cacgcatcgg ctttggcgca agcgggcgca atggcgggca acttgtgaac
    13201 tcctacagcc gcgacatcga cgtgatcgaa aaaagctacg gcatggacac cgcccgtatg
    13261 ctcggcagca tgatgttcga gggtggcgag atcatccgcg aacgtatcaa acgctatcag
    13321 attgactgcg actatcgccc cggcggcctg tttgtggcga tgaatgataa acagctcgcc
    13381 acactcgaag agcagaaaga gaactgggaa cgctacggca ataaacagct ggagctgctg
    13441 gacgctaacg ccattcgccg tgaagtagcc agcgatcgct acactggtgc gctgctggat
    13501 cacagcggtg ggcatattca cccgctaaac ctcgccattg gtgaagccga tgccatccgc
    13561 ctgaacggcg ggcgcgtgta tgagctttct gcggtcacgc agatccagca caccacgccc
    13621 gccgtggtgc gtactgccaa aggtcaggtg acggcgaagt atgtgattgt cgccgggaat
    13681 gcgtacctgg gcgataaagt ggagccggag ctggcgaaac gcagcatgcc atgcggtacg
    13741 caggtgatca ccaccgagcg gctgtcggaa gatttagccc gttcgctgat cccgaaaaac
    13801 tactgcgtgg aagactgtaa ttatctgctt gattactacc gtcttaccgc cgataaccgc
    13861 ctgctgtacg gcggcggcgt agtgtatggc gcgcgcgacc cggatgacgt tgagcgtctt
    13921 gtggtgccga aactgctgaa aaccttcccg cagttgaagg gcgtgaaaat cgactatcgc
    13981 tggacgggca acttcctgct gaccctgtcg cgtatgccgc agtttggtcg cctcgatacc
    14041 aacatctatt acatgcaggg ctacagcggc cacggcgtca cctgtactca tctcgccgga
    14101 cgtttgattg ccgaactgct gcgcggcgac gccgaacgtt tcgatgcctt cgccaatctg
    14161 ccgcattacc cgttccccgg cgggcgcacg ctgcgcgtac cgtttaccgc gatgggcgcg
    14221 gcgtattaca gcctgcgcga tcgtctgggc gtttaatttc cgattaacag tgaagagtca
    14281 aaaggtgtga aacatgagca acaatgaatt ccatcagcgt cgtctttctg ccaccccgcg
    14341 cggggttggc gtgatgtgta acttcttcgc ccagtcggct gaaaacgcta cgctgaagga
    14401 tgttgagggc aacgagtaca tcgatttcgc cgcaggcatt gcggtgctga ataccgggca
    14461 tcgccaccct gatctggtcg cggcggtgga gcagcaactg caacagttta cccacaccgc
    14521 gtatcagatt gtgccgtatg aaagctacgt caccctggcg gagaaaatca acgcccttgc
    14581 cccggtgagc gggcaggcca aaaccgcgtt cttcactacc ggcgcggaag cggtggaaaa
    14641 cgcggtgaaa atggctcgcg cccataccgg acgacctggc gtgattgcgt ttagcggtgg
    14701 cttccacggt cgtacatata tgaccatggc gctgaccgga aaagttgcgc cgtacaaaat
    14761 cggcttcggc ccgttccctg gttcggtgta tcacgtacct tatccgtcag atttacacgg
    14821 catttcaaca caggactccc tcgacgccat cgaacgcttg tttaaatcag acatcgaagc
    14881 gaagcaggtc gcggccatta ttttcgaacc ggtgcagggc gagggcggtt tcaacgttgc
    14941 gccaaaagag ctggttgccg ccattcgccg cctgtgcgac gagcacggta ttgtgatgat
    15001 tgccgatgaa gtgcaaagcg gctttgcgcg taccggtaaa ctgtttgcca tggatcatta
    15061 cgccgataag ccggacttaa tgacgatggc gaaaagcctc gcgggcggga tgccgctttc
    15121 gggcgtggtc ggtaacgcga atattatgga cgcgcccgcg cccgcgccgg gcgggttggg
    15181 tggcacctac gccgggaacc cgctggcggt ggctgccgcg cacgcggtgc tcaatattat
    15241 cgacaaagaa tcactctgca aacgcgcgaa tcaactgggc cagcgtctca aaaacacgtt
    15301 gattgatgcc aaagaaagcg ttccggccat tgctgcggta cgcggcctgg ggtcgatgat
    15361 tgcggcagag tttaacgatc cgcaaacggg cgagccgtca gcggcgattg cacagaaaat
    15421 ccagcaacgc gcgctggcgc aggggctgct cctgctgacc tgtggcgcat acggcaacgt
    15481 gattcgcttc ctgtatccgc tgaccatccc ggatgcgcaa ttcgatgcgg caatgaaaat
    15541 tttgcaggat gcgctgagcg attaatagcc caacgcaata atgtctgatg cgctgcgctt
    15601 atcaggcctg caaacgacgt tgattatgta tgccggataa agcattcacg ccgcatccgg
    15661 caagttgtat tgcccaactt cgctaaatct ggtgcttttt caacaacgcg cggaactgat
    15721 gataggttaa ccccagtaat tcagccgcgc gcttctggtt atatttccct tgttgcaaac
    15781 tgagttgcag caactctttt tcctgctgca tctgaaactc acgtaaatcc agcggcagtg
    15841 ttggaagcga ggtggtttct gaaacggcga tagcgtcttc aggcggacgc cgtttaaagg
    15901 gatcaataat gatgtcatca agcggataat cgctggtgcc gtggcgatac actgaacgtt
    15961 ccaccacgtt tttcaattca cgaatatttc ccggccaacg ataattcagc aatgtttctc
    16021 tggcgcgctc cgtaaacccc gggaacagag gcagcttgat ttcccgacac atctggatgg
    16081 caaagtgttc tgccatcaac ataatgtcgc tttcgcgctc gcgcagtggt ggcagttgta
    16141 caacatcaaa agccagtcgg tcgagcaggt cagcgcgaaa agtgccttca ttgaccatcg
    16201 ccgggagatc ggcattcgtc gcgcatacca accgcacatt cacctgcaat ggctggctgc
    16261 cgccaacgcg ctccagttca ccgtactcaa tcacgcgcaa taatttctcc tgcaccatca
    16321 tcggtgccgt agcgagttca tcaagaaata gcgtaccgcc gtcggcacgt tcaaatctcc
    16381 ctggatgacg tttttgcgca ccggtaaacg cccccgcttc gtgaccaaac agttcggaat
    16441 ccagcagatt ttcatttaac gccgcgcagt taagggaaat aaacggccct tgccaacggg
    16501 aggagagata atgcaggcgg ctggcaatca gctctttacc tgttccgcgc tcgccgatga
    16561 tgagcaccgg tttgtccagc ggtgcgagat gcgaaacctg ttccagcact tcgagaaagc
    16621 tgttcgcctc accgagtaaa ttatctttgt attctgccat gatgaaattc gccacttgtt
    16681 agtgtaattc gctaactcat cctggcatgt tgctgttgat tcttcaatca gatctttata
    16741 aatcaaaaag ataaaaaatt ggcacgcaaa ttgtattaac tgttcagcag gacaatcctg
    16801 aacgcagaaa tcaagaggac aacattatgg gtattttttc tcgctttgcc gacatcgtga
    16861 atgccaacat caacgctctg ttagagaaag cggaagatcc acagaaactg gttcgtctga
    16921 tgatccagga gatggaagat acattggttg aagtacgttc tacttcggcg cgcgcgttgg
    16981 cagaaaagaa acagctgact cgccgtattg aacaagcgtc ggcgcgtgaa gttgaatggc
    17041 aggaaaaagc cgaactggcg ctgctgaaag agagagagga tctggcgcgt gcggcgttaa
    17101 ttgaaaaaca gaaactgacc gatctgatta agagcctgga acatgaagtg acgctggtgg
    17161 acgatacgct ggcacgcatg aagaaagaga ttggcgagct ggaaaacaaa ttgagcgaaa
    17221 cacgcgctcg ccagcaggca ttgatgttac gccatcaggc ggcaaattcg tcgcgcgatg
    17281 tgcgtcgtca gttggacagt ggcaaactgg atgaagcaat ggctcgtttc gaatctttcg
    17341 aacgtcgtat tgaccagatg gaagcagaag cggaaagcca cagcttcggt aaacaaaaat
    17401 cgctggacga tcagtttgcc gaactgaaag ccgatgatgc aataagcgag caactggcac
    17461 aattaaaagc caaaatgaag caagacaatc aataataaca tccaggcggc gtccgaaagc
    17521 gccgccgctc atcgtctaag gagtacttat gagcgcgcta tttctggcta ttccattaac
    17581 catttttgtg ctgtttgttt taccgatctg gttatggctg cattacagca atcgttctgg
    17641 tcgcagtgaa ttgtcgcaaa gtgagcagca gcgattagcg caactggctg atgaagcaaa
    17701 acggatgcgc gaacgtattc aggcgctgga atctattctt gatgcagaac atccgaactg
    17761 gagggatcgc taatggcggg cattaatctt aataaaaaat tatggcgtat tccgcagcag
    17821 ggtatggtac gcggcgtctg cgccgggatt gccaactatt ttgatgtacc ggtaaaactg
    17881 gtgcgtatcc tggtggtact gtcgattttc ttcggtctgg cgctgtttac cctggttgct
    17941 tacatcattt tgtcatttgc gcttgatcca atgccggaca acatggcctt tggtgagcag
    18001 ctaccttcca gcagcgaatt gctggatgaa gtcgaccgtg aactggcggc aagtgaaacg
    18061 cgtttacgcg agatggaacg ttatgtcact tccgatacat tcacgttacg tagccgtttc
    18121 cgtcaactgt gaggaaagtt atgaatactc gctggcaaca ggccgggcaa aaggtaaagt
    18181 ctggtttcaa attagcaggc aagctggtac ttcttaccgc actgcgctat ggcccagcgg
    18241 gtgtggcggg ctgggcgata aaatcagttg ctcgccgacc gctgaaaatg ttgctggctg
    18301 tggcgctgga accgctgtta agtcgggctg ccaataaact ggcgcagcgt tataaaaggt
    18361 gaggggagtt tcgcaaaaat tgttaaatct caggcctata atggatggca attttcatcc
    18421 atagaaggac gcttacatgt ttaaaaaagg cttacttgct ctggcactgg tgttttcact
    18481 gcccgttttc gccgctgaac actggatcga tgttcgtgtt ccagagcagt atcagcaaga
    18541 gcacgttcag ggggccatca atattcccct gaaggaagtg aaagagcgca ttgccaccgc
    18601 cgttccggat aaaaacgaca ccgtgaaagt gtattgcaat gccggacgcc agtccgggca
    18661 ggcaaaagag atccttagcg agatgggata tacccacgtt gagaacgccg gtggcctgaa
    18721 agacatcgca atgccgaagg tcaaaggtta aaagaattca ccatgagcgg cgcttatgcg
    18781 ccgttttttt tctgtcacac cttatttaca tccccataga ttatttgcgt cagctcacaa
    18841 atacgctttt cccctggtaa aaaatgattt cctgcgtgac taaaaccctt gtgctcaatt
    18901 gacagtttat tttctgcgga gtagtctctc gtttcatggg accgctacca cggaaaggca
    18961 acatgaaaca gaaaattacg gattacctgg acgaaatcta cggtggaaca tttaccgcaa
    19021 ctcatttaca gaaacttgta acgcgtcttg agagtgcgaa acgattaatt acacagcgac
    19081 gtaaaaaaca ctgggatgaa agtgatgtcg tgttaattac ctatgccgat caatttcaca
    19141 gcaatgattt aaaaccatta cccacattta atcagtttta ccatcaatgg ctgcaaagca
    19201 ttttttcaca tgttcatttg ttgccgtttt atccatggtc atctgatgat ggcttttcgg
    19261 taattgatta tcatcaggtc gccagtgaag cgggggagtg gcaggatatt cagcaactcg
    19321 gtgaatgcag tcatttaatg tttgattttg tctgcaacca tatgtcggca aaaagtgaat
    19381 ggtttaaaaa ctatttacaa cagcatccag gttttgaaga tttttttatt gccgttgacc
    19441 cgcaaaccga tctcagcgcc gtcactcgcc cgcgtgcgtt accgttatta acgccattcc
    19501 agatgcgcga tcattcaacg cgccatttat ggaccacctt tagtgacgat caaattgacc
    19561 tgaattaccg tagccctgaa gtgttgctgg cgatggtgga tgttttactc tgttaccttg
    19621 cgaaaggtgc tgagtatgtc cgcctggatg ccgttggctt tatgtggaaa gagccgggaa
    19681 caagctgcat ccatctggaa aaaacacatc tgattatcaa actgttacgg tcgattattg
    19741 ataacgttgc gccaggtaca gtgatcatta ccgagaccaa tgttccgcat aaagacaaca
    19801 ttgcttactt tggcgcaggc gatgacgaag cacatatggt gtaccagttc tcgctgccgc
    19861 cactggtgct gcatgcggtg caaaaacaga acgttgaggc gctttgtgcg tgggcgcaaa
    19921 acctgacact accttccagc aacaccacct ggtttaactt cctcgcctct cacgatggca
    19981 tcgggctaaa cccgctacgg ggcttgttgc ctgaaagcga aatattagag ctggtcgagg
    20041 cgttacagca ggaaggtgca ttagtaaact ggaaaaataa tcccgacggt acacgcagtc
    20101 cgtatgaaat aaatgtgacc tatatggatg cgttaagccg ccgtgagagt agcgatgaag
    20161 aacgttgcgc caggtttatc cttgcccatg cgattttgtt aagtttcccc ggtgtgccag
    20221 cgatatatat tcaaagtatt cttggctcgc gtaatgatta cgcaggtgtc gaaaaactcg
    20281 gatataaccg tgcgattaac cgtaaaaaat atcacagtaa agagataacc cgagaactga
    20341 acgatgaagc tacattaagg catgcggtat atcatgagtt gtcgcgttta attacacttc
    20401 gtcgcagcca taacgagttt catccggata ataattttac cattgatacg attaatttat
    20461 ccgtaatgcg tattcaaaga agtaacgctg atggtaattg tctgactgga ttgtttaatg
    20521 tcagtaaaaa tattcagcat gtaaatatta ctaatctgca tggtcgggat ctgattagtg
    20581 aagttgatat attgggtaat gaaataacgc tgcgcccctg gcaggttatg tggattaaat
    20641 aaaaaggaac atctcatgat taaatcaaaa atcgtgctgt tatcagcact ggtttcatgc
    20701 gccctgattt caggctgtaa agaagaaaat aaaacgaatg tatccatcga atttatgcat
    20761 tcttcggtgg agcaggagcg ccaggccgtt atcagtaaat tgattgcccg ttttgaaaaa
    20821 gaaaaccctg gcatcaccgt taagcaagtg cccgtggaag aagatgccta taacactaaa
    20881 gtcattactc tttcacgtag cggttcgctg ccggaagtga tcgaaaccag ccatgactac
    20941 gccaaagtga tggacaaaga gcagcttatc gatcgcaaag cggttgccac agtcatcagc
    21001 aacgttggtg aaggcgcgtt ttacgatggc gtactgcgta ttgtgcgtac cgaagatggt
    21061 agcgcatgga ccggtgttcc tgtcagcgcc tggattggcg gtatctggta tcgcaaagat
    21121 gtgctggcaa aagcggggct tgaggagccg aaaaactggc aacagctgct ggacgttgca
    21181 cagaaactga atgacccggc gaataaaaaa tacggcattg cgctgcctac agcagaaagc
    21241 gtgttgacgg aacaatcctt ctcccagttt gcgttatcca accaggctaa cgtctttaac
    21301 gccgaaggca aaatcaccct tgatacacca gagatgatgc aggcactgac ctattaccgc
    21361 gaccttgctg ccaacactat gccgggttct aacgacatca tggaagtgaa agacgccttt
    21421 atgaacggca ccgcgccgat ggcgatttac tccacctata tccttccggc tgtgattaaa
    21481 gaaggcgacc cgaaaaacgt cggtttcgtg gtgccaaccg agaaaaactc tgcggtctac
    21541 ggcatgttga cctcgctgac cattaccgcc gggcaaaaga ccgaagagac ggaagcagca
    21601 gaaaaatttg tcacctttat ggagcaggca gacaacattg ccgactgggt gatgatgtcg
    21661 ccaggtgctg cgctgccggt gaataaagcg gtggtgacta ccgccacctg gaaagacaac
    21721 gacgttatta aggcgctggg tgaactaccg aatcagctaa tcggtgaact gccaaatatt
    21781 caggtttttg gcgcagtagg ggataaaaac tttacccgca tgggtgatgt gacgggttct
    21841 ggcgtggtga gttcaatggt gcataacgtc accgtgggta aagccgatct ctctactacg
    21901 ctgcaagcga gccagaaaaa actggatgaa ctgatcgaac agcactaaac ccaggacagg
    21961 aatccgcaat gaacaggctt ttttcaggtc gttccgatat gccctttgcg ctgctgcttc
    22021 tcgcgcccag cttattactg ctgggcggtc tggtggcgtg gccgatggtg tcgaatatcg
    22081 aaatcagttt tttacgtctg ccgctcaatc ccaacatcga gtcaacgttt gttggggtga
    22141 gcaactatgt gcgtatcctc tccgatcccg acttctggca ttcgctgtgg atgacggtct
    22201 ggtataccgc gctggtggtg gcgggcagca ccgtgctcgg gctggcggtg gcgatgtttt
    22261 tcaaccgcga gttccgcctg cgcaaaaccg cgcgttcgct ggtgatcctc tcctacgtaa
    22321 cgccgtccat ttcgctggtg ttcgcctgga aatacatgtt caacaacggc tacggcattg
    22381 ttaactacct cggcgtcgat cttctgcatc tctatgagca ggcaccgctg tggttcgata
    22441 atccgggcag tagctttgtg ctggtggtgc tgttcgccat ctggcgctac ttcccgtatg
    22501 cctttatctc gtttctggcg attttgcaga ccatcgacaa atcgctgtac gaagcggcag
    22561 agatggatgg cgctaatgcc tggcaacggt ttcgtatcgt cacgctgccc gcaattatgc
    22621 cggtcctggc gacggtggtg acactgcgca ccatctggat gttctacatg ttcgcggatg
    22681 tttatttgct gacgaccaag gtcgatattc tcggtgtata tctctacaaa accgcctttg
    22741 cctttaatga tttaggaaaa gcggcggcga tctcggtggt gctcttcatc attattttcg
    22801 ctgtcattct gctgaccagg aaacgggtga acctcaatgg caacaaataa acgcacactc
    22861 agtcgcatcg gtttttactg cgggctggcg ctgtttctca tcatcacgct gtttccattt
    22921 tttgtgatgc tgatgacctc gttcaagggc gcgaaagagg cgatctcact gcatcctacg
    22981 ctgctgccgc agcagtggac gctggagcat tacgtcgaca tttttaaccc gatgattttc
    23041 ccgtttgtcg actacttccg taacagtctg gtggtgtctg tggtttcatc cgtggtggcg
    23101 gtattcctcg gcattcttgg ggcttacgcg ctttcccgcc tgcgctttaa aggtcggatg
    23161 accatcaacg ccagctttta cacggtgtat atgttctctg gcattttgct ggtggtgccg
    23221 cttttcaaaa tcatcaccgc gcttggcatt tacgacactg agatggcgct gatcatcacc
    23281 atggtgacgc aaactctgcc caccgccgtg tttatgctga aaagctactt cgacaccatc
    23341 cccgatgaga tcgaagaagc ggcgatgatg gatggcctca accgtctgca aattatcttc
    23401 cgcatcaccg tgccgttggc gatgtccggg ctgatatccg tgttcgtcta ctgctttatg
    23461 gtggcgtgga acgactatct gtttgcgtcg attttcctct ccagcgccag caatttcacc
    23521 ttaccggtgg gcctgaacgc gctgttcagt acgccagatt acatctgggg acggatgatg
    23581 gcggcctcac tggtgaccgc attgccggtg gtgattatgt atgcgctttc ggaacgtttt
    23641 attaagagtg gtttgaccgc cggtggcgtg aagggctaaa gcggccagtt tctttacaag
    23701 gagttttaaa tgaaaaagtt agtagccaca gcaccgcgtg ttgctgcgct ggttgagtat
    23761 gaagatcggg cgattttagc taatgaagtg aagatccgcg tgcgtttcgg cgcaccgaaa
    23821 cacggaacgg aagtggtcga cttccgcgcc gccagcccgt ttattgatga agactttaac
    23881 ggcgaatggc agatgttcac tccgcgtccg gcagatgcgc cgcgcggcat tgagtttggc
    23941 aaattccagc ttggcaacat ggtggttggc gacattatcg agtgcggcag cgacgttacc
    24001 gactacgcgg tgggcgacag cgtatgcggc tacggcccgc tctccgagac ggtcatcatt
    24061 aacgcagtga ataactacaa gctgcgcaaa atgccgcaag gcagctcctg gaaaaacgcc
    24121 gtctgctacg acccggcgca gtttgccatg agcggcgtgc gcgatgccaa cgtgcgcgta
    24181 ggggattttg tggtggtggt agggcttggc gcaatcggtc aaattgccat ccaactggct
    24241 aaacgcgctg gcgcttctgt ggtgattggc gtcgatccta tcgcccatcg ctgtgatatt
    24301 gcccgtcgcc acggcgcgga tttctgcctt aaccccatcg gcactgatgt aggtaaagag
    24361 atcaaaacgc tgaccggcaa gcagggtgcc gatgtgatta tcgaaaccag cggctacgcc
    24421 gacgcgctgc aatcggcgct ccgcggtctg gcttatggcg gcaccatctc ctatgtcgcg
    24481 tttgccaagc cgtttgccga aggttttaac ctcggacgcg aagcgcattt caataacgcc
    24541 aaaattgtct tctctcgcgc gtgcagcgaa ccgaacccgg attatccgcg ctggagccgc
    24601 aagcgtattg aagaaacctg ttgggaactg ctgatgaacg gttatctcaa ttgcgaagat
    24661 ttaatcgacc cggtggtgac ctttgccaat agcccggaaa gctatatgca gtatgtcgac
    24721 cagcatccgg aacagagcat caaaatgggc gttacgtttt aattcaaagg aatgaaaaaa
    24781 atgaaaatcg gcacacagaa tcaggcgttc tttccggaaa acattctgga gaaatttcgt
    24841 tatatcaaag agatgggctt cgatggtttt gagattgacg gcaaactgct ggttaacaac
    24901 atcgaagaag tcaaagcggc gatcaaagaa accggtttac cggtgaccac cgcctgcggt
    24961 ggctatgacg gatggattgg cgactttatc gaagagcgtc gtcttaatgg cttaaagcag
    25021 atcgagcgca ttctcgaagc gctggcagaa gtgggcggta aaggcatcgt cgttccggct
    25081 gcgtggggca tgtttacctt ccgcttaccg ccgatgacct cgccgcgtag cctggacggc
    25141 gaccgcaaaa tggtgagtga ttccctgcgc gtactggaac aggtcgccgc gcgtaccgga
    25201 accgtggtgt atctcgaacc gttaaaccgc tatcaggatc atatgatcaa caccctcgcc
    25261 gatgcccgcc gttacatcgt cgaaaacgat cttaaacatg tacagattat cggcgatttc
    25321 tatcacatga atatcgaaga agataacctg gcgcaggcgc tgcatgacaa ccgcgacctg
    25381 ctcggtcatg tgcatattgc ggataaccat cgctaccagc cgggcagcgg caccctggat
    25441 ttccacgcgc tgtttgaaca gctgcgcgcg gataactatc agggctatgt agtgtacgaa
    25501 gggcgtatcc gggcggaaga tcctgcccag gcgtaccgtg attcgttggc ctggttgcgt
    25561 acctgctaag aggtctttgt gaaaagtgca atgacaagct ctccgctgcg ggtcgcgata
    25621 ataggcgcag gccaggtggc ggataaggtt catgcttcgt actactgcac ccgcaacgat
    25681 ctggaactgg tggctgtctg tgacagccgc ctttcccagg cgcaggcgct ggcagaaaaa
    25741 tacgggaatg catccgtgtg ggacgatccg caggccatgc tgctggcggt gaaacctgat
    25801 gtggttagcg tctgctcacc taaccgtttt cattacgaac ataccctgat ggcactggaa
    25861 gcgggctgcc atgtgatgtg cgaaaaaccg cccgccatga cgccagaaca ggcgcgggaa
    25921 atgtgcgata ccgcgcgcaa actgggcaag gtgctggcct acgactttca ccatcgtttt
    25981 gcgctcgata cgcaacagct gcgtgaacag gtgaccaacg gcgttttggg agagatttac
    26041 gttaccaccg cccgcgccct gcgtcgctgc ggcgttcccg gctggggtgt ctttaccaat
    26101 aaagaactgc agggtggtgg cccgctgatc gacatcggca ttcatatgct ggatgctgcg
    26161 atgtatgtgc tgggctttcc ggcggtgaaa agcgtgaatg cgcatagctt tcaaaagatc
    26221 ggcacgcaaa agagctgtgg tcaatttggt gagtgggatc cggcaactta cagcgtcgaa
    26281 gattcgctgt ttggcaccat tgaatttcat aacggcggca ttctgtggct ggaaacgtca
    26341 tttgcactca acatccgcga acagtcgatt atgaacgtca gcttttgtgg tgataaagct
    26401 ggtgcgacgc tgtttccagc acatatctac accgataaca acggtgaatt aatgacgctg
    26461 atgcaacggg aaatggcaga cgacaaccgc catttgcgca gcatggaagc ctttatcaat
    26521 cacgtacagg gcaagcccgt gatgatagcc gacgccgagc aggggtacat catccagcaa
    26581 ctggtggcgg cgttgtatca atccgcagaa acagggacgc gtgtggaatt atgaccaggc
    26641 cagtaacgtt atcagaaccc catttcagcc agcataccct gaacaagtat gcatcgctga
    26701 tggcgcaggg gaacggctat cttgggcttc gcgccagcca tgaagaagat tacacgcgcc
    26761 agacgcgagg gatgtatctg gcggggctgt atcatcgggc gggaaaaggt gaaatcaacg
    26821 aactggtgaa cctgcctgat gtcgtgggga tggagattgc cataaatggt gaggttttct
    26881 cgttatccca cgaagcctgg cagcgtgagc ttgactttgc cagtggcgaa ttacgccgca
    26941 atgttgtctg gcgtaccagc aacggctcag gttacaccat cgccagccgt cgctttgttt
    27001 cggcagacca actgccgctc attgcgctgg aaatcactat tacgccactg gacgccgacg
    27061 cgtcagtgct gatttcaaca ggcattgacg ccacgcaaac caatcacggt cgccaacatc
    27121 tcgacgaaac ccaggtgcgg gtgtttggtc agcatctgat gcaggggagc tacaccaccc
    27181 aggatggacg cagtgatgtg gccatcagct gttgctgtaa ggtgagcggt gatgtgcagc
    27241 aatgctatac cgccaaagag cgccgtttac tgcaacatac cagtgcgcag cttcatgcag
    27301 gcgagacaat gacgttgcaa aaactggtgt ggatcgactg gcgggatgac aggcaagctg
    27361 ctttagacga gtggggcagc gcgtcgcttc gccagcttga aatgtgcgcg cagcagagtt
    27421 acgaccaact tcttgcagca tcaacagaaa actggcgtca atggtggcag aaacgtcgta
    27481 tcacggtaaa tggcggcgaa gcgcacgatc agcaagcgtt agattatgcg ctttatcatc
    27541 tgcgcatcat gacgcctgcc cacgacgagc gcagcagcat tgcggcaaaa ggcttaaccg
    27601 gcgaaggcta caaaggccac gttttctggg atacagaagt atttttgtta ccgtttcatc
    27661 tgtttagcga tccgacggtt gcccgaagtt tactgcgtta tcgctggcac aacttgccag
    27721 gcgcgcagga gaaagcgcga cgcaacggct ggcagggcgc gctatttccg tgggaaagcg
    27781 cgcgcagcgg cgaagaagag acgccggaat ttgccgccat taacattcgc accgggctgc
    27841 ggcaaaaagt ggcctcggcg caggcggaac atcatctggt ggccgatatc gcctgggcgg
    27901 ttattcaata ctggcagacc acgggggatg aaagtttcat tgcgcatgaa ggcatggcgc
    27961 tacttctgga gacggcaaag ttctggatta gccgcgcggt gagagttaac gatcgtctgg
    28021 aaattcatga tgttattggg ccagacgaat ataccgaaca tgtcaataat aatgcataca
    28081 ccagctatat ggcccgctac aacgttcaac aggcgctgaa tattgcccgc cagttcggct
    28141 gtagcgacga tgcgtttatc catcgcgccg aaatgttcct caaagagcta tggatgccag
    28201 aaattcagcc cgacggcgtt ttgccgcagg atgattcgtt tatggctaag ccggcgatta
    28261 atctggcgaa atacaaagcg gcggcgggga agcaaaccat actgctggat tattcacgcg
    28321 cagaagtgaa cgagatgcag atcctcaaac aagctgatgt ggtgatgctc aattacatgc
    28381 tgccggagca gttctcagcg gcatcgtgtc ttgccaatct gcaattttat gaaccgcgca
    28441 ctattcacga ctcgtcatta agtaaagcaa tccacggcat tgttgccgca cgctgtggcc
    28501 tgctgaccca aagttatcag ttctggcgcg aggggactga aatcgatctt ggtgctgatc
    28561 cgcatagttg tgatgatggt atccatgctg ccgcaactgg cgctatctgg ctgggggcga
    28621 ttcagggttt tgccggggtg agcgtgcgtg acggtgaatt gcatctcaat ccggcgttac
    28681 ctgagcagtg gcaacagttg tctttccctc tgttctggca gggctgcgaa ttacaggtca
    28741 ctcttgacgc gcagcgtatt gcgattcgaa cttctgcgcc cgtttcactg cgtttgaacg
    28801 gtcagcttat aaccgtggct gaagaatctg ttttctgttt gggtgatttt attttgccct
    28861 tcaatgggac cgctaccaaa catcaggagg atgaatgaaa ctgcaagggg taattttcga
    28921 tctggatggt gtaatcaccg ataccgcgca tctgcatttc caggcgtggc agcagattgc
    28981 cgctgaaatt ggcatcagca ttgatgcgca gtttaacgaa tccctaaaag ggatcagccg
    29041 cgatgagtct ctgcggcgca ttctgcaaca cgggggcaaa gagggcgact ttaactcgca
    29101 ggagagggcg caactggcgt atcgcaaaaa tctgctctat gtccactcac tacgcgagtt
    29161 gacggtcaac gctgttctac ccggcattcg ctctttgctg gcagatctcc gtgcacagca
    29221 gatctcggtt gggctggctt ctgtctccct gaatgcgccg acgattttag cggcgctgga
    29281 gctgcgcgag tttttcacct tctgcgcgga tgcttcccaa cttaaaaact cgaaaccgga
    29341 cccggaaatc tttctcgccg cctgtgcagg gctgggcgtg ccgccgcagg catgtatcgg
    29401 cattgaagat gcgcaggcgg gcattgacgc cattaacgcc agcggtatgc gctcggtggg
    29461 gatcggcgcg ggcttaaccg gggcgcaatt actgttgcct tcaacggaat cactcacctg
    29521 gccgcggtta tcggccttct ggcaaaacgt atagcaaagg aatcaacatg gctcagcttt
    29581 cgttacaaca tattcaaaaa atctacgata accaggtgca tgtggtgaag gacttcaacc
    29641 tggaaattgc cgataaagag ttcatcgtgt ttgtcggccc gtcgggctgc ggtaagtcga
    29701 ccaccctgcg catgattgcc gggcttgagg agatcagcgg cggcgatctg ttgatcgacg
    29761 gcaaacgaat gaatgacgtt ccagccaaag cacgcaatat agcgatggtg ttccagaact
    29821 acgcgttgta tccgcatatg acggtttacg acaacatggc gtttggtctg aagatgcaaa
    29881 aaatcgccaa agaggtgatt gatgagcggg tgaactgggc ggcgcaaatt ctcggactgc
    29941 gtgagtacct gaaacgtaag ccgggggcgc tttccggcgg gcaacgtcag cgagtggcgc
    30001 ttgggcgggc gattgtacgc gaagcgggcg tgtttttaat ggatgaaccg ctctctaacc
    30061 ttgatgccaa gctgcgcgtg caaatgcgcg cagagatcag caagctgcat cagaaactga
    30121 acaccaccat gatctacgtg acccacgatc agaccgaagc gatgaccatg gcgacgcgga
    30181 ttgtgattat gaaagacggg attgttcagc aagtaggtgc gccgaaaacc gtttataacc
    30241 aacccgcgaa tatgtttgtt tccggattta ttggatcacc agcgatgaat tttattcgcg
    30301 gcacgatcga tggcgataaa ttcgttacgg aaacgcttaa attaaccatt cccgaagaga
    30361 aattagcggt tctgaaaaca caggaaagtt tgcataagcc catcgtgatg ggaatacgac
    30421 cggaagatat tcatccggac gcgcaagagg aaaataacat ttccgccaaa attagcgtgg
    30481 cagaattaac cggtgcggaa tttatgctct acaccacggt tggggggcac gagttagtgg
    30541 tccgtgctgg tgcgttaaat gattatcatg caggagaaaa tatcactatt cattttgata
    30601 tgacgaaatg tcatttcttt gatgcagaaa cggaaatagc aattcgctaa atacaggggg
    30661 aaggcattcc cccaggataa tacaaggaac aataatgaaa aagttattac cctgtaccgc
    30721 actggtgatg tgtgcgggaa tggcctgcgc acaggccgag gaaaggaacg actggcactt
    30781 taatatcggc gcgatgtacg aaatagaaaa cgtcgagggt tatggcgaag atatggatgg
    30841 gctggcggag ccttcagtct attttaatgc cgccaacggg ccgtggagaa ttgctctggc
    30901 ctattatcag gaagggccgg tagattatag cgcgggtaaa cgtggaacgt ggtttgatcg
    30961 cccggagctg gaggtgcatt atcagttcct cgaaaacgat gatttcagtt tcggcctgac
    31021 cggcggtttc cgtaattatg gttatcacta cgttgatgaa ccgggtaaag acacggcgaa
    31081 tatgcagcgc tggaaaatcg cgccagactg ggatgtgaaa ctgactgacg atttacgttt
    31141 caacggttgg ttgtcgatgt ataaatttgc caacgatctg aacactaccg gttacgctga
    31201 tacccgtgtc gaaacggaaa caggtctgca atataccttc aacgaaacgg ttgccttgcg
    31261 agtgaactat tatctcgagc gcggcttcaa tatggacgac agccgcaata acggtgagtt
    31321 ttccacgcaa gaaattcgcg cctatttgcc gctgacgctc ggcaaccact cggtgacgcc
    31381 gtatacgcgc attgggctgg atcgctggag taactgggac tggcaggatg atattgaacg
    31441 tgaaggccat gattttaacc gtgtaggttt attttacggt tatgatttcc agaacggact
    31501 ttccgtttcg ctggaatacg cgtttgagtg gcaggatcac gacgaaggcg acagtgataa
    31561 attccattat gcaggtgtcg gcgtaaatta ctcgttctga taatgggcta aattgccgga
    31621 tgcggcgcga gtactttatc cgatctataa atgtaggccg gataagatgc gctagcatcg
    31681 catccggcat tcaggcaagg tagctggtat ttatttcagc gtcatatgcg tggcaacggt
    31741 aatattctgt ggtgacggtt ttccagaaat taagcggaat aataactcgc agctttgttg
    31801 acctaactcc tgcgtcggaa catcgatgcc gcccggtgca ggcgttaaaa taaatgacag
    31861 cgtttcattg ctataaccca ccaccgctaa ctgctgcgga atagcaatat ttttctctgc
    31921 tgccgcacga taaatgctca ttaatttcag gctgtcagtg gcaaacaccg cctcaggcaa
    31981 cggtgactgg cttaataatt gccgtgctgc ttttaatgca gtttcatggg tataaccgcc
    32041 atcaacaatc cattcatcac gcactgcaat attatgcgca gccaggctct gcttataacc
    32101 attaacgcga tcaaccgaaa catggacatc aagcggagca tgcaggcagg caatattttg
    32161 atgcccactt tcaattaatg catcggtcaa cgcaatgctg tcgccaaaat tatcggtatc
    32221 gacagaataa acatgggcat attgaccttc aactttgcca atcaccacca ccggaatatc
    32281 atatttatcg agttgggcaa aaaatgactc atccgctggc gaactgagca taataatgcc
    32341 tttaatcatt ttctgcttaa ttttgctttc gcatttttgc agatcttctg ccggattgtg
    32401 cgacgtttgt aatatcacgt cgaaaccttc ttcttcagct ttggcggtga tggcatgcaa
    32461 aacttcagag aaaaacggat tacccgccgt agttttggtc gaacgggtag aaatcaccat
    32521 aatggcatca aaacccgaag aggtcagcgc gcgggccagc ttgtttggct gatactgtaa
    32581 ttcttcaatg gcccgtaaca ctttttcgcg cgcttccggg gagatattag tttgcttatt
    32641 cagcacgcgt gatacggtgg attttgatac gcctgcaacc ctggcaatat cataaatagt
    32701 aggggacata ggtcaggaac tccgtccgaa cgtgaatgct gcacatctta tggagttcgc
    32761 cggtcgatga caacacgggc agttgataat caatggcctg gcccccacat tcatatcctt
    32821 acgaatgatt ttttttctgg tcttcagagc agcacaggac agcaatgaag cgacttaaaa
    32881 atgaactcaa tgcgctggtg aatcggggtg tcgacagaca tctgcgcctc gctgtaaccg
    32941 gacttagccg cagcggcaaa acagcgttta tcaccgcgat ggttaatcag ttgcttaata
    33001 ttcacgcccg agcacgtttg ccgctgttaa gtgcggtgcg tgaagagcgc ctgctgggcg
    33061 tgaaacgcat tccccagcgt gactttggca ttccgcgctt cacctatgac gaagggctgg
    33121 cgcagttata tggcgatcca cccgcctggc caacgccaac gcgcggcgtc agtgaaatcc
    33181 gcctggcgct acgttttaaa tcgaatgatt cgctgctacg ccactttaag gatacctcca
    33241 cgctgtatct ggaaattgtg gattatcccg gcgaatggtt gctcgacctg ccgatgctgg
    33301 cgcaggacta tttaagctgg tcgcgccaga tgacgggctt actcaatggt cagcgcggag
    33361 aatggtcggc gaaatggcga atgatgagcg aagggctgga cccgctagcg cctgccgacg
    33421 aaaaccggct ggcggacatt gccgccgcgt ggaccgatta tctccaccac tgtaaagagc
    33481 aggggctgca ctttattcag cctgggcgct ttgtcttgcc gggagatatg gcaggtgcgc
    33541 ccgcgctgca attcttcccg tggccggatg tcgatacctg gggcgagtcc aaactggcgc
    33601 aggccgataa gcataccaat gccggaatgc tgcgcgagcg ctttaattat tactgcgaga
    33661 aggtggtgaa ggggttctat aagaatcatt ttctgcgctt tgaccgccag attgtgctgg
    33721 tggattgcct gcaacctctc aacagtgggc cacaggcatt taatgatatg cgtctggcac
    33781 tgacgcagct gatgcaaagt tttcactacg ggcagcgtac cctgttccgg cgtttgtttt
    33841 cgccggttat cgataagcta ttgtttgctg ccactaaagc ggaccatgtg accatcgatc
    33901 agcacgctaa tatggtttca ttactgcaac aactgattca ggatgcctgg caaaatgcgg
    33961 cgttcgaagg gatcagcatg gactgcctgg ggctggcgtc agttcaggcg accaccagcg
    34021 gcattattga tgttaacggt gagaaaatcc cggcgctgcg tggtaatcga cttagcgatg
    34081 gcgcaccgct cactgtttat cctggcgaag ttcccgcacg tttgcctggt caggcgttct
    34141 gggataagca aggcttccag tttgaggcat ttcgtccgca ggtgatggat gtcgacaaac
    34201 cactaccgca tattcgtctt gatgctgcgc tggaattttt aataggagat aaattgcgat
    34261 gaccgaaccg ttaaaaccac gtattgattt cgacggtcct ctggaggtcg atcagaatcc
    34321 taaattcagg gcgcagcaga cctttgacga aaatcaggcg caaaattttg ccccggccac
    34381 gctcgacgaa gcgcaggaag aagaggggca agtcgaagcg gtaatggacg cagcgttacg
    34441 tccgaaacgc agcctgtggc gcaaaatggt gatgggcggg ctggctctgt ttggcgcaag
    34501 cgttgtcggg cagggtgtac agtggacaat gaatgcctgg caaacccagg actgggtggc
    34561 gctgggtgga tgtgccgctg gggcattgat tatcggcgct ggcgtaggtt ctgtggtaac
    34621 agagtggcgg cgcttatggc gcttgcgaca gcgcgcccat gaacgcgacg aagcgcgtga
    34681 tttattgcat agccacggca cgggcaaagg ccgcgcattt tgcgaaaaac tggcgcagca
    34741 ggcgggtatt gatcagtcgc atccggcgct gcaacgctgg tatgcctcaa tccatgaaac
    34801 gcaaaacgac cgtgaagtgg tcagcctgta tgcgcatttg gtccagccag ttttagatgc
    34861 ccaggcgcgg cgcgaaatca gccgttcggc ggcggaatca acgttgatga ttgcggtcag
    34921 cccgctggcg ttggtcgata tggcgtttat cgcctggcgc aatctgcgtt taattaatcg
    34981 catcgccacg ctgtatggca ttgaactggg gtattacagc cgtttgcgtc tgtttaagct
    35041 ggtattgctg aatatcgctt ttgccggagc cagcgaactg gtgcgcgaag tggggatgga
    35101 ctggatgtcg caagatctcg ctgctcgttt gtctacccgc gcagctcagg ggattggtgc
    35161 aggacttctg acggcacgac tcgggattaa agctatggag ctttgccgcc cgctgccgtg
    35221 gattgacgat gacaaacctc gcctcgggga tttccgtcgt cagcttatcg gtcaggtgaa
    35281 agaaacgctg caaaaaggca aaacgcccag cgaaaaataa tgcaatatcg ggtgctgacc
    35341 ggatatcttt acgccgaagt gcccgttttt ccgtctttgt gtcaatgatt gttgacagaa
    35401 accttcctgc tatccaaata gtgtcatatc atcatattaa ttgttctttt ttcaggtgaa
    35461 ggttcccatg cgtctggaag tcttttgtga agaccgactc ggtctgaccc gcgaattact
    35521 cgatctactc gtgctaagag gcattgattt acgcggtatt gagattgatc ccattgggcg
    35581 aatctacctc aattttgctg aactggagtt tgagagtttc agcagtctga tggccgaaat
    35641 acgccgtatt gcgggtgtta ccgatgtgcg tactgtcccg tggatgcctt ccgaacgtga
    35701 gcatctggcg ttgagcgcgt tactggaggc gttgcctgaa cctgtgctct ctgtcgatat
    35761 gaaaagcaaa gtggatatgg cgaacccggc gagctgtcag ctttttgggc aaaaattgga
    35821 tcgcctgcgc aaccataccg ccgcacaatt gattaacggc tttaattttt tacgttggct
    35881 ggaaagcgaa ccgcaagatt cgcataacga gcatgtcgtt attaatgggc agaatttcct
    35941 gatggagatt acgcctgttt atcttcagga tgaaaatgat caacacgtcc tgaccggtgc
    36001 ggtggtgatg ttgcgatcaa cgattcgtat gggccgccag ttgcaaaatg tcgccgccca
    36061 ggacgtcagc gccttcagtc aaattgtcgc cgtcagcccg aaaatgaagc atgttgtcga
    36121 acaggcgcag aaactggcga tgctaagcgc gccgctgctg attacgggtg acacaggtac
    36181 aggtaaagat ctctttgcct acgcctgcca tcaggcaagc cccagagcgg gcaaacctta
    36241 cctggcgctg aactgtgcgt ctataccgga agatgcggtc gagagtgaac tgtttggtca
    36301 tgctccggaa gggaagaaag gattctttga gcaggcgaac ggtggttcgg tgctgttgga
    36361 tgaaataggg gaaatgtcac cacggatgca ggcgaaatta ctgcgtttcc ttaatgatgg
    36421 cactttccgt cgggttggcg aagaccatga ggtgcatgtc gatgtgcggg tgatttgcgc
    36481 tacgcagaag aatctggtcg aactggtgca aaaaggcatg ttccgtgaag atctctatta
    36541 tcgtctgaac gtgttgacgc tcaatctgcc gccgctacgt gactgtccgc aggacatcat
    36601 gccgttaact gagctgttcg tcgcccgctt tgccgacgag cagggcgtgc cgcgtccgaa
    36661 actggccgct gacctgaata ctgtacttac gcgttatgcg tggccgggaa atgtgcggca
    36721 gttaaagaac gctatctatc gcgcactgac acaactggac ggttatgagc tgcgtccaca
    36781 ggatattttg ttgccggatt atgacgccgc aacggtagcc gtgggcgaag atgcgatgga
    36841 aggttcgctg gacgaaatca ccagccgttt tgaacgctcg gtattaaccc agctttatcg
    36901 caattatccc agcacgcgca aactggcaaa acgtctcggc gtttcacata ccgcgattgc
    36961 caataagttg cgggaatatg gtctgagtca gaagaagaac gaagagtaag cgcgaatatg
    37021 cctgatggtg caacaccatc aggcatatta aattatgctt tcagtacagc cagagctgct
    37081 tcgtaatccg gctcggtggt gatttcatcc accagctggc tgaaaatcac attgtcattt
    37141 tcgtcaataa ccacaacggc acgcgctgcc agacctttca gtgggccatc agcaattgcc
    37201 acaccgtagg cttgcagaaa ttcagcgtta cggaaagtgg agagggtgat aacgttgttc
    37261 agaccttctg cgccgcagaa acgagactgg gcgaacggca gatcggcaga gatacacagc
    37321 acaacggtgt tgtcgatctc ggttgccagt tggttaaact tacgtactga tgcggcgcaa
    37381 acaccggtat caatactcgg gaaaatgttc agcactttgc gtttacccgc aaactgaccg
    37441 agggtgacgt cagacagatc ttttgccacg agagtaaaag tctgcgcttt gctacccgcc
    37501 tgcgggatgg aattggcgac tgtaaccggg ttgccctgga aatgaacggt ttgtgacatg
    37561 attatctttc ctgtttacat atagttaacg tcacacctag tttatgctaa ctgtcaataa
    37621 cacagcaaac gctatttgcg cttcatccgc agaccaccgc gacaacaagg agtaaagatg
    37681 agaaccgtta aggtattcga ggaagcctgg cccttacata ccccgtttgt gattgcccgg
    37741 ggaagtcgca gtgaagcgcg cgtggtggtg gttgaactgg aagaagaggg tattaaaggc
    37801 accggcgaat gcacgccgta tccgcgttat ggggaaagtg atgcctcggt aatggcgcaa
    37861 attatgagcg tcgtgccgca actagagaaa gggctgacac gggaggagtt gcaaaaaatt
    37921 ctccctgccg gcgcagcacg taatgcgctg gattgtgcat tgtgggatct ggccgcgcga
    37981 agacagcagc aatcgctggc tgatttgatc ggcataacgc ttcccgagac agttatcact
    38041 gcacagacgg ttgtcatcgg tacgcctgat cagatggcca atagtgcatc aacactctgg
    38101 caggcaggcg cgaaattact gaaagtgaag ctggataacc atcttatcag tgagcggatg
    38161 gtggcaattc gcacagctgt gcccgatgcg acgctgatcg ttgatgcaaa tgaatcctgg
    38221 cgtgcagaag ggttggcggc gcgttgccag ctattggcgg atttaggcgt tgcgatgctt
    38281 gaacaaccgc ttcctgcgca ggacgatgcg gcgctggaga attttattca tccgttgccg
    38341 atttgtgctg atgaaagttg tcatactcgt agcaatttga aggcgctgaa agggcgctat
    38401 gagatggtta atattaagct cgataaaacc gggggtctga cggaagcgct ggcgctggcg
    38461 actgaagcgc gtgcacaagg tttcagtctg atgctgggct gcatgttgtg tacctctcgt
    38521 gccattagcg ccgctttacc gctggtgccg caggtcagtt tcgccgatct tgacggaccg
    38581 acctggctgg cggtagatgt ggaaccggcg cttcagttca cgacgggcga attgcatctt
    38641 taggatgcca gcgcagcaag tttgccatcg caaagagata tttttcgctg gcttcgtcgg
    38701 aggagatggg cggaaactct gcggtgatgc aatgcaagtt cagatctgca caccaactgc
    38761 cgaaagagcc aggcgtttca taaccgacgc tggttaccag aggcaattca aacgcctgag
    38821 ccagccattc gcctaattcg ctgtgtctgg gatcttcaat acaggccagt ggatcgtgga
    38881 atgagaccac ccaggcaggt tgaatgcggt gtataagctg gcacagcgcc tgggtttcag
    38941 gttcggagcc aggtttgtca cctgtcagta atacgacatc gcgttcttca gcggcgctgt
    39001 tccagcgata aacggtttca ccttccttcc agttcgccgc cggaaagttt ctgtttaaat
    39061 ccacaccatt agcattggcc cgtaacccca actggcagcc gtcaggattc acacacagca
    39121 ccacatgatg acggcgcaaa gaaggtgtca acgtccgcag agcacaggag agcgtgacga
    39181 ccgaagagtt ttcatcaccg tgagtgccag ccagaatcaa accactttcg cggctggctg
    39241 caggggcagg aaaccagatt aacggcgcgc ccaataatga acgtccgtaa tgttctgttc
    39301 cgggcggaaa tgcgccgcgt tcggcgcgtg ggcgggttac ggtcatgatc gtctctggtt
    39361 caggaagtat tacaggcagt gttgtgcaaa ttttgcggat tatcaaatcc ttttcttcaa
    39421 cgctatgtca tcgatcttgt atcacttttt tgccggaagt tgtttgcatt ttattagttg
    39481 gatagggttt gtggattttt gctggctgac gggcaaataa agtttgttta atggtttgct
    39541 tatctgccag ttgattaatc acatggcggg ctatctgacc gccagcacca agaatcagta
    39601 catttttcat gaaaattatc ccagattatt ggcagcaata tgccaggcca tatcactatt
    39661 gcggatttga atatgtttca ttacagagtt tcctcaaaga acggagctaa tactgaaatt
    39721 gcttcagcca cataggcttt gccgtcataa agatccatat ggtttgcgcc ttcaacaatg
    39781 tgatagcgtt tatcctggct tgatgctcga tcgtacaggt cgtcactcat ccatttgctc
    39841 cctgcctggc tgcccgccac aatctgcgtc ggctgagtca ggtacacttc cgccatatgg
    39901 taagcatcat aggtaataat ctggttaagg ctgcgcaaag tagcgtaacc cggtgctgtt
    39961 ggatactgcg cgcgaggggt gtggtaatac tcccaggcct gacgcagttc ttcgttcggc
    40021 gcatcggact ccttcattgg tgccagtggc ataatggcgt attctccgct gctaatatcg
    40081 ctggttctgg cgtttgaacc cgcttcaacg tatggcaggg catcaataga tttcacattg
    40141 ttttcccaac cattacggaa tatcgaacca atattgaccg cactaacggt accgatggcc
    40201 ttgatgcggc gatcctgaat tgcagcattg gctgtatatc ctgcaccggc acaaattccc
    40261 atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg tcaggtaatc aatcacggca
    40321 ctgatgtctt cagtacgaat gtatgggttt tctaactgac gcggctcgcc gccactttca
    40381 ccttgataag atgcgtcata agcaatagtg acaaatccct tttccgccag ttttttggca
    40441 taggttccgg ccgtttgttc tttaacgccc ccacctgggt gagataacac aattgcctga
    40501 tactgacggg tttcatcaaa ttttggaggg aaatagatca ctgcagacaa agagatggtt
    40561 ggattattgc tgttagtgaa gctgacttta ttattcatca ttccgttcct catggagttg
    40621 tcggttcgtt ttaacggttg gtgatatcac tatagatatt gatcattaag ttgattagac
    40681 ccaaaatcat gattagacct atcatttaaa tgattaatag attgcatgga gatactgaat
    40741 gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc gttgcagagg agcgtagctt
    40801 cactcgtgca gcagcccgcc tgagcatggc gcagtcagct ttaagccaga tagtgcgtcg
    40861 tatagaagaa cgattgggat tgcggcttct gacgcgaacc acgcgcagcg ttgttccaac
    40921 tgaagcgggc gagcatcttt tgtctgttct tggccctatg ttgcatgaca tagattcagc
    40981 catggcatcc ctgagcgatc tgcagaaccg cccatccggg acaatacgta ttactactgt
    41041 agaacatgca gcaaaaacga tattgttacc agcaatgcgc acattcctga aatcgcatcc
    41101 tgaaattgat attcagctca ccattgatta tggtttgacc gatgtcgttt ctgaacgttt
    41161 tgatgcaggc gtccgtctgg gtggggagat ggataaagat atgatcgcca ttcgaatcgg
    41221 gccagatata ccaatggcta ttgttggctc accggattat ttttctcgcc gaagtgttcc
    41281 aacgtcagtg tcacaattaa tagatcatca ggcaattaat ttgtatcttc ccacatcggg
    41341 tacagcaaat cgctggagat taatacgcgg tggacgtgaa gttcgtgttc gcatggaagg
    41401 tcagctttta ctgaatacga tagacctgat cattgatgct gcaattgatg ggcatggatt
    41461 ggcgtatcta ccttatgatc aggttgagcg ggctattaaa gaaaaaaaac tgatacgtgt
    41521 tttggataaa ttcacaccag atttacccgg ttatcacctg tactatccac accgtcgaca
    41581 tgctggctcg gcattctcat tatttataga taggctgaag tataaaggtg ctgtttagca
    41641 ctacttgctg atacattaat ttaattcttc tcttaacgta ttctcagttc ctttcaacgt
    41701 tttggtcatt ttttattctt cgtacaatgg cgacagatgc tgattatgat accgaaaacg
    41761 ggtttgaacg tgcgaagccc gaacgtagtg ttcggagttc tatgtgcttt accgcatttt
    41821 ggagactatt atttacacta aatctgattt gatatattga tacttaaaac atttgatgct
    41881 tcctttgtca cttttttgat ggaagttgtt tgcatttctt taaggcgaaa caaataatta
    41941 cgcatcaatt ttaatgtcgg ttagagggaa acttatgaag cactctgttt cagtcacgtg
    42001 ttgtgcgctg ttggtcagca gcatttctct ttcgtatgct gcagaagttc cgagcggcac
    42061 agtactggca gagaagcagg agctggtgcg ccacattaaa gatgagcctg cgtcgctgga
    42121 tcccgctaaa gccgtgggcc tgccagagat tcaggtcatt cgcgatctgt ttgaaggtct
    42181 ggtgaatcag aacgaaaaag gggagattgt ccccggcgtt gcgactcagt ggaaaagtaa
    42241 tgacaaccgt atctggactt ttaccctgcg cgataacgca aaatgggcgg atggcacacc
    42301 ggtaacggcg caagattttg tctacagctg gcaacgtctg gtggacccaa aaacattgtc
    42361 gccatttgca tggtttgccg cgctggcggg aatcaacaac gcacaggcga ttattgatgg
    42421 taaagctacg cctgaccagc ttggcgtcac cgcagttgat gcccatactt tgaaaattca
    42481 gcttgataaa ccgttgccgt ggtttgtgaa tttaaccgct aactttgcct tcttcccggt
    42541 gcaaaaagcc aacgtagaaa gcggtaaaga gtggacgaaa cccggaaatc tgatcggcaa
    42601 tggcgcttat gttcttaaag agcgcgtagt caatgaaaaa ctggtcgtgg taccgaatac
    42661 ccattattgg gataacgcca aaacggtact gcaaaaagtg accttcctgc caattaatca
    42721 ggaatccgca gccactaagc gttacctcgc gggggatatt gatatcaccg aatccttccc
    42781 gaaaaatatg tatcagaagc tgttgaagga tattccgggg caggtttata cgccgccgca
    42841 gctcgggacc tattattatg cgtttaacac gcaaaaaggg ccgacggcag atcagcgcgt
    42901 tcgtctggca ttaagtatga cgatagatcg ccgcctgatg accgaaaaag tattagggac
    42961 gggcgaaaag ccagcgtggc attttacacc agatgttacc gcgggattta cgccggaacc
    43021 ttcgccgttt gaacaaatga gtcaggaaga actgaatgcg caggcaaaaa ctttgttgag
    43081 cgcagctggt tatggtccgc aaaaaccgct gaagctgacg cttttgtata acacttcaga
    43141 aaaccatcaa aaaattgcga ttgctgtagc atcgatgtgg aaaaagaacc ttggcgtaga
    43201 tgttaaattg caaaatcagg aatggaaaac ctatatcgat agccgtaaca ccggcaattt
    43261 tgatgttatc cgcgcctcgt gggtggggga ttataatgaa ccctccactt tcctgacatt
    43321 attaacgtca acgcattcag gaaatatttc acgctttaac aatccggcat atgacaaagt
    43381 tctggcccag gcatcgacgg aaaataccgt taaagcgcgt aatgccgatt acaacgcggc
    43441 agaaaaaatc ctcatggagc aagcaccgat tgcaccaatt tatcaatata ccaatggacg
    43501 attaatcaag ccgtggctga aaggttatcc cattaataat cctgaagatg tggcgtacag
    43561 tcggactatg tatattgtga agcattgatg tgatgggaac tggcgttacc cttgtgcata
    43621 acgccagtga tgtctgattt agcgaccctg ttccggcggt gtaatgttat ccatatacag
    43681 cgtctggctg gggaaggcaa agtccgcgcc gtgtgactgt acaatatcga taatcttcaa
    43741 ataaacgtct tgctgtgcag caagccattc agcccatacc gtggttttgg taaagcaata
    43801 aaccataata ttcaatgaag agtcagcaaa ctggttgaaa taaaccagta aggtttgtcg
    43861 ctggtcgatg gccgggtgat ttttcagcat ctcacgtaca gcttcgacaa taacgcccac
    43921 ttttgccgca tcctcataac gtaaaccaat ggtcgtggta atgcggcggt tggtcattcg
    43981 tcctgggttt tctacgctga tcgacgaaaa cagcgagttc ggtacgtaca atggacgatt
    44041 atcaaa
//
LOCUS       DACAPB010000011        42930 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.011, whole genome shotgun sequence.
ACCESSION   DACAPB010000011 DACAPB010000000
VERSION     DACAPB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.011"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..261)
                     /locus_tag="IT904_000554"
     CDS             complement(<1..261)
                     /locus_tag="IT904_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190387.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLN"
     gene            complement(553..2412)
                     /gene="gss"
                     /locus_tag="IT904_000555"
     CDS             complement(553..2412)
                     /gene="gss"
                     /locus_tag="IT904_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAP3190388.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEK
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2617..3483
                     /gene="yghU"
                     /locus_tag="IT904_000556"
     CDS             2617..3483
                     /gene="yghU"
                     /locus_tag="IT904_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAP3190389.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3606..3854)
                     /gene="hybG"
                     /locus_tag="IT904_000557"
     CDS             complement(3606..3854)
                     /gene="hybG"
                     /locus_tag="IT904_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAP3190390.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3867..4208)
                     /gene="hypA"
                     /locus_tag="IT904_000558"
     CDS             complement(3867..4208)
                     /gene="hypA"
                     /locus_tag="IT904_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP3190391.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(4201..4689)
                     /gene="hybE"
                     /locus_tag="IT904_000559"
     CDS             complement(4201..4689)
                     /gene="hybE"
                     /locus_tag="IT904_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAP3190392.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4682..5176)
                     /locus_tag="IT904_000560"
     CDS             complement(4682..5176)
                     /locus_tag="IT904_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAP3190393.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(5176..6879)
                     /gene="hybC"
                     /locus_tag="IT904_000561"
     CDS             complement(5176..6879)
                     /gene="hybC"
                     /locus_tag="IT904_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAP3190394.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6876..8054)
                     /gene="hybB"
                     /locus_tag="IT904_000562"
     CDS             complement(6876..8054)
                     /gene="hybB"
                     /locus_tag="IT904_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAP3190395.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(8044..9030)
                     /gene="hybA"
                     /locus_tag="IT904_000563"
     CDS             complement(8044..9030)
                     /gene="hybA"
                     /locus_tag="IT904_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAP3190396.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGGNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPQYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(9033..10151)
                     /gene="hybO"
                     /locus_tag="IT904_000564"
     CDS             complement(9033..10151)
                     /gene="hybO"
                     /locus_tag="IT904_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAP3190397.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLAGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10340..10627)
                     /locus_tag="IT904_000565"
     CDS             complement(10340..10627)
                     /locus_tag="IT904_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAP3190398.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10746..11630)
                     /locus_tag="IT904_000566"
     CDS             complement(10746..11630)
                     /locus_tag="IT904_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP3190399.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLMN
                     DFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADVA
                     KIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDKS
                     AADLAWQRTLKWFDKYLS"
     gene            11787..12827
                     /gene="gpr"
                     /locus_tag="IT904_000567"
     CDS             11787..12827
                     /gene="gpr"
                     /locus_tag="IT904_000567"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP3190400.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12867..13361)
                     /locus_tag="IT904_000568"
     CDS             complement(12867..13361)
                     /locus_tag="IT904_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAP3190401.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13552..14436
                     /gene="yghA"
                     /locus_tag="IT904_000569"
     CDS             13552..14436
                     /gene="yghA"
                     /locus_tag="IT904_000569"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP3190402.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14708..15133)
                     /gene="exbD"
                     /locus_tag="IT904_000570"
     CDS             complement(14708..15133)
                     /gene="exbD"
                     /locus_tag="IT904_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAP3190403.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(15140..15874)
                     /locus_tag="IT904_000571"
     CDS             complement(15140..15874)
                     /locus_tag="IT904_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAP3190404.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            16126..17313
                     /gene="metC"
                     /locus_tag="IT904_000572"
     CDS             16126..17313
                     /gene="metC"
                     /locus_tag="IT904_000572"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAP3190405.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17453..18112
                     /locus_tag="IT904_000573"
     CDS             17453..18112
                     /locus_tag="IT904_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP3190406.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(18152..19051)
                     /locus_tag="IT904_000574"
     CDS             complement(18152..19051)
                     /locus_tag="IT904_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP3190407.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            19245..20408
                     /gene="yqhD"
                     /locus_tag="IT904_000575"
     CDS             19245..20408
                     /gene="yqhD"
                     /locus_tag="IT904_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP3190408.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20513..21340
                     /gene="dkgA"
                     /locus_tag="IT904_000576"
     CDS             20513..21340
                     /gene="dkgA"
                     /locus_tag="IT904_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAP3190409.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21540..22466
                     /locus_tag="IT904_000577"
     CDS             21540..22466
                     /locus_tag="IT904_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAP3190410.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22517..22774
                     /gene="yqhH"
                     /locus_tag="IT904_000578"
     CDS             22517..22774
                     /gene="yqhH"
                     /locus_tag="IT904_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAP3190411.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22817..25036)
                     /locus_tag="IT904_000579"
     CDS             complement(22817..25036)
                     /locus_tag="IT904_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAP3190412.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMAIQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(25147..26559)
                     /gene="ftsP"
                     /locus_tag="IT904_000580"
     CDS             complement(25147..26559)
                     /gene="ftsP"
                     /locus_tag="IT904_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAP3190413.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTSEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26634..27371)
                     /gene="plsC"
                     /locus_tag="IT904_000581"
                     /gene_synonym="parF"
     CDS             complement(26634..27371)
                     /gene="plsC"
                     /locus_tag="IT904_000581"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAP3190414.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27605..29863)
                     /gene="parC"
                     /locus_tag="IT904_000582"
     CDS             complement(27605..29863)
                     /gene="parC"
                     /locus_tag="IT904_000582"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP3190415.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQCIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30001..31608)
                     /locus_tag="IT904_000583"
     CDS             complement(30001..31608)
                     /locus_tag="IT904_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP3190416.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31741..32136)
                     /gene="mqsA"
                     /locus_tag="IT904_000584"
     CDS             complement(31741..32136)
                     /gene="mqsA"
                     /locus_tag="IT904_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HAP3190417.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(32138..32434)
                     /gene="mqsR"
                     /locus_tag="IT904_000585"
     CDS             complement(32138..32434)
                     /gene="mqsR"
                     /locus_tag="IT904_000585"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HAP3190418.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32639..33121)
                     /locus_tag="IT904_000586"
     CDS             complement(32639..33121)
                     /locus_tag="IT904_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP3190419.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(33174..33566)
                     /gene="ygiW"
                     /locus_tag="IT904_000587"
     CDS             complement(33174..33566)
                     /gene="ygiW"
                     /locus_tag="IT904_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAP3190420.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33718..34377
                     /gene="qseB"
                     /locus_tag="IT904_000588"
     CDS             33718..34377
                     /gene="qseB"
                     /locus_tag="IT904_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAP3190421.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            34374..35723
                     /gene="qseC"
                     /locus_tag="IT904_000589"
     CDS             34374..35723
                     /gene="qseC"
                     /locus_tag="IT904_000589"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAP3190422.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35769..36102)
                     /locus_tag="IT904_000590"
                     /pseudo
     CDS             complement(35769..36102)
                     /locus_tag="IT904_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
     gene            36421..37002
                     /gene="mdaB"
                     /locus_tag="IT904_000591"
     CDS             36421..37002
                     /gene="mdaB"
                     /locus_tag="IT904_000591"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAP3190423.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEDHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            37033..37347
                     /locus_tag="IT904_000592"
     CDS             37033..37347
                     /locus_tag="IT904_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP3190424.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(37395..39287)
                     /gene="parE"
                     /locus_tag="IT904_000593"
     CDS             complement(37395..39287)
                     /gene="parE"
                     /locus_tag="IT904_000593"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP3190425.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLAAQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(39316..39897)
                     /gene="yqiA"
                     /locus_tag="IT904_000594"
     CDS             complement(39316..39897)
                     /gene="yqiA"
                     /locus_tag="IT904_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAP3190426.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(39897..40724)
                     /gene="cpdA"
                     /locus_tag="IT904_000595"
     CDS             complement(39897..40724)
                     /gene="cpdA"
                     /locus_tag="IT904_000595"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAP3190427.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(40749..41171)
                     /locus_tag="IT904_000596"
     CDS             complement(40749..41171)
                     /locus_tag="IT904_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAP3190428.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(41172..41801)
                     /gene="nudF"
                     /locus_tag="IT904_000597"
     CDS             complement(41172..41801)
                     /gene="nudF"
                     /locus_tag="IT904_000597"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAP3190429.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKLVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            42006..>42930
                     /gene="tolC"
                     /locus_tag="IT904_000598"
     CDS             42006..>42930
                     /gene="tolC"
                     /locus_tag="IT904_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAP3190430.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGL"
BASE COUNT        10641 a        11418 c        10692 g        10179 t
ORIGIN      
        1 attcagcaac aaatcggttg gcaacatatg gacaatggca taggcaacgc taagtccgcc
       61 caataacacg ccaaaaaagt taaaaaatgc cgccatcacc acagcaagtt gtggttgcat
      121 ggcacgagta taaataacgg ttgccaccgc attcgccgtg tcatgaaaac cattgattgc
      181 ttcgtagaac aacacaaatg ccagagcaag taataacaaa agccctgtgt atatatcaag
      241 gccaacaaat aaatttagca tatattagat tacgccattt tgaatttacg aacggacgca
      301 ttatcagtga ctttaacggt gtgggcaaag tgaaatatca ttttttaacg ataaaaagag
      361 tgttatttct tgtgtatcag acaataagca ttcattaaaa atctttagaa ataattagaa
      421 cacaggcatt ctgctggtta atgtattcac atatgaaatg aataaatatt tgaatatata
      481 aatattgaat attttgatta atccccgtac tgactattct tcataatcag tacgggattg
      541 ctacaacatg tattactctt tcaccacaat taacggttca atatcactct cttttttaat
      601 aaccagtgat tcatcaccgc gcaaacacgt cccaccgtag ttgccgccaa cggtgaaggt
      661 acatacctga atgtatttcc cgtccacttt cggcaaacac cacagttgct gatagatgtt
      721 tttctgctcg gcaaatttac cgctggtttt gtccagcacc tcttcatgat ggctgacgag
      781 gtcgatgtta ctgccacagc gaccggcgat cggtttcact gcgtaacctg ttttcaccaa
      841 ttcatcatta acagtgaaat cggtatccag caggtaacga tggtgcggga acagcgacca
      901 gaggatcggc agaatcgctt tgttgccggg gatcaccgtc cacagcggct caaagactag
      961 cacttccggg cgcagcaata cgtcgataag acgcacttca ttttgcggat gaccggtacg
     1021 gatcggcacc gcagcaaact cacggtcgct aacttcacga atctgatcaa acgcggtttc
     1081 ccacgcccag gttttccaca cgcagttaac cagtcgccct tccccatcaa tcagttgccc
     1141 ggcagcatcc cagcccagtt catccagccc gcgcaagata cgcgtttcaa agcccgcctg
     1201 gtgcagcgcc tgctccataa actgcgcgtg atagttttcc tcgatatctt tgtcctgcat
     1261 gatatggaca aacggacgtg cacgactgtg tttccaggca ccagccagtt cgttaatcag
     1321 cccttccgcc ggattgaatc cgttgccttt atagccctgc tccgcccaac gttcgaggat
     1381 caagcccgct tcggtatgac aggaggcgga atcggcgttg tattcgtaga ccttcaggcc
     1441 acgttcgtcc atacagaaat ccatacgacc ggtgatcata tggtgacggc gacgctgcca
     1501 ggagagacgc aaacgtggcc agaggatttt tgggatgtcg aacagcgcca gcaggttgtc
     1561 atctttcagc accttgtcgg tggcgtgaag atacatcagg tgcagctcgt tggtggcttt
     1621 aatcagctcc tgctcggcgc tctcggtaat ggtgtagtaa tgatacggat cctgattgat
     1681 cacctgaccg ttggcctgca cataggcgtt ttgcagcgga tctttttcat ccagccattt
     1741 accgtcaaac tggccttttt tttccagacg cgctccgctg attttcagca gctcgcctgc
     1801 aatttccggc tgcggtaagc tgtattcagt atcttctgtc tggatcatcc agcccagaat
     1861 ggtggtgtca tcaaaagtgt ctttaagggt atagcagccg ttttcgacca ccatctccag
     1921 ttcgcgcgtc cactgttgcc cttgcggcaa cggggaatga atcacgttct gttccgcaat
     1981 acggactttg ttgccatgca attgggtaat gatggcgaca tggccagtgt ctttaaattc
     2041 accgccttta tcccagataa gaagcgcacc cgcgaccggc gcacgcggcg agccgttagg
     2101 aaatgcctgc aatggcagga tgttgtcatt aaccacttca cgcaggaagc gcagcgagaa
     2161 aatctcccac gccataccca cgtcagtaaa gaccacaccg taattcagaa agagaaaacg
     2221 gcgagcaaat tcaacgcatt gccacttgtg gcccatatat tcgtcgtcga tatagctacg
     2281 gaatacggcg tcatcttcgt attcctgcgg atcgagagaa ctgtaatctg aagagtagat
     2341 tgctacccca cctggggcgt agcccaataa tgtcccgaac ggggcatcct ggctggtcgt
     2401 tcctttgctc atcactttac ctttaacaat acaacctaag cagttggcgt aattgttgtg
     2461 ctctgattac ctgctcagcg acattaaccg gacagaggtc agactaatca tacacctcat
     2521 cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg tacagcaagt gacctgctac
     2581 actgcttcaa cactaccact cagaaggcaa ctcactatga cagacaatac ttatcagccc
     2641 gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt tcgccaatat caatcgcccg
     2701 gtttctggtc cgacgcatga aaaaacgctg ccggtgggca aacatccatt acaactttat
     2761 tcgctgggaa cgccgaacgg tcagaaagta acgattatgc ttgaggagct gctggcgctg
     2821 ggcgttactg gtgcagagta cgacgcctgg ctgattcgta ttggcgatgg cgatcaattc
     2881 tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc cggcgctgcg cgatcatacg
     2941 cataatccgc cgatccgcgt gtttgaatct ggttcgatcc tgctttatct ggcggagaaa
     3001 tttggctact tcctgccgca ggatttggca aagcgtactg aaacgatgaa ctggctgttc
     3061 tggttacagg gcgcggcacc gttcctcggc ggtggttttg gtcactttta ccattacgca
     3121 ccggtaaaga ttgagtacgc catcaaccgc tttaccatgg aagccaaacg tctgctcgac
     3181 gtgctggata agcaactggc gcagcataag tttgttgcgg gcgatgagta caccattgcg
     3241 gatatggcga tttggccgtg gtttggcaac gtggtgttag gtggtgtgta tgatgccgct
     3301 gagtttcttg atgcgggcag ttataagcat gtacaacgct gggcgaaaga agtaggcgaa
     3361 cgtccggcgg tgaaacgtgg gcgtattgtt aaccgcacca acggaccgct gaatgagcag
     3421 ttgcatgagc gccatgacgc cagtgatttc gagacgaata cggaagataa gcgtcagggg
     3481 taagggttgg tgttcgtcgc agcaagccat ccaggccgga taaggcgttc acgccgcatc
     3541 cggcaatcgt gcacaatgcc tgatgcgacg ctgccgcgtc ttatcaggcc tacgaaagct
     3601 aatcatcacg cgctggtaat gtcgtaatcc atttggcgca gtgcgtctaa tgtggctttg
     3661 gcttcatctt cgtcgatgat gctcatggca aatccgacgt gtaccagcac ccactggccc
     3721 agtagatcgg cagggttacc ttcacaaatc agggcgatat tcacatcgcg cttgatacca
     3781 catacttcaa cctgcgcaag ctggtgaata tcttcaccga cagccagcac ctggcctgga
     3841 acgccaatac acattattaa ctccggttat tcaacttcaa tacttttgac gatcagcgaa
     3901 tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc atcgtgctga
     3961 tgaatctcca ccacctggct gcaatcccag caccaagcct gggcgggttt atagacgata
     4021 tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact aaaacggacg
     4081 gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt gacgcgctta
     4141 acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca aagagacaac
     4201 tcatgcattt tcgccactcc tgcgaccaaa cagcagggcg cgacgccctg cgtgtggtac
     4261 atccggattc gtgactggca gcgaaaggat cattcgtgcg cagtcatccg tcaggcgttg
     4321 gccctcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg agagatattg
     4381 cgaaacaccg tccagttcgc caacggtaaa ggtcatagtg ccatacggca gttgcagacc
     4441 aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga agataactgc
     4501 actcagcatc cacggggtga tcacacaccc cgtccactga ccttcgaaca gcgtaaaatc
     4561 agaaacatac accggcattg aaggatgcag aaaagagaga tcgtgcatcg aacgccgggc
     4621 aatttcttca aacgctgcct gtacttgcgc cttcggggag gtctggaaac ctgctatctc
     4681 ttcagtcatg aatagcctcc cgtgggatgg cttccacgcc agattcacgc agcgcagcca
     4741 gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag ccgatgtgtg
     4801 gctccagcga ttccgggatc acgccgacca gggtcagctt tttcggaaac tcgccggtga
     4861 agcgcagggc cgacaagacg tcggccaggc caagctgatg cggagagatt ttgttggtaa
     4921 acaacgccgg aacttcttca tcccgcagga tcatcatcgt tcccggcgcg ttctttttcg
     4981 agacaatggc atccgcaata atcagatgat cgcgatttgc catgtcgcca agcagctcca
     5041 ttcccgccgt gccgccatcg aggatctcaa cataatccgg cagaatgtat cgttgctcta
     5101 acgcttcgac aatccgcaca ccgatggctt catcggtcag caaaatattg ccgaccccta
     5161 agactaaaat acgcattaca gaaccttcac tgaaaccact tcgttgccgt cagcatccac
     5221 tacgtgtacc gcacaggcca tgcacgggtc aaaggagtga atggtacgca ccacttccag
     5281 cggtttattc ggatcggcaa ccggtgtacc caccagcgac tgctcgtaag gaccgacgtc
     5341 atcattgaag ttacgcggac cagagttcca ggttgatgga acaaccgcct ggtagttgct
     5401 gatgataccg tctttaataa ccatccagtg agagagcata ccgcgcggcg cttcgaggaa
     5461 gccaacacct ttgaattcac ccgttgccgg aatgttcggc ttcacaaagg tggtgtgatc
     5521 gcctttgccg atattggtga tcagtgcact gtattggttt tgcaggatat cctgcaattc
     5581 acagcagtga acggtacgac caataatacg gcccagcgtg gagtgcagct gtgccacttc
     5641 cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt tcattcagtt tgttctgggt
     5701 agattcgcga cctgccgcca gtttcaccag catattagcc agtggcccca cttctaccgt
     5761 tttgccgtag aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag accaaccatc
     5821 ataagccgga atggtggtgc cttcccacgg tgcctgcggc gcttcgtctt tataccagga
     5881 gtgcttcgcg ctttcctgaa tgcctttgat caggtattca tcggaatgag aagtgatcgg
     5941 acgatacgag gacagatccg cattctcaat gtagccgccc gggaacagga agctgccgtt
     6001 tttactgtcg gtcgggaatt ccggcacgct caggtagttc accgcacctt taccgcgtgt
     6061 cagccattcc gggtagaacg cggcaataac tgcggtatca accttataaa cctgctcaac
     6121 aaagtcgctc agtttgtcga tgaaagactt gatgtacatc aggcgctcaa ggttcagcac
     6181 gcccaaaccg tcgaggttga tcgggttcgc gacaccacct accgccaggt tctgaatgtg
     6241 cggcgtttta ccgcccagca gcgccacgac gcggttagcg tcacgctggc actccaacgc
     6301 ttgcaggtag tgcgctaccg caatcaggtt cacttccggc ggcagtttca tcgccggatg
     6361 accccagtag ccattagcga aaatacccaa ctgaccgctg gcaaccagat ctttgatctt
     6421 gttctgaact ttggtgaact cttccggact gttcaggtgc caggtcgaaa cgcctttcag
     6481 catttcggag gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca cccagtccag
     6541 cgccgaaagc tgatagaaat gaacaatatg gtcatgcgtg gtgtgcgcag ccagaatgat
     6601 gttacggatg tattgcgcgt taaccggaac gtcgatattc agcgcacttt ctgccgcacg
     6661 aacggaagac agcgcgtgag tggtagtaca tacgccacag atacgttgca caatcatcca
     6721 tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca tggtaccgga
     6781 agcccatgct tttgaaacga cgccattttc gatttcgcaa tcgatgcgta aatgcccctc
     6841 aatacgggtt accggatcaa tagtaattct ctggctcatg ctttgctcgc ctcatgacga
     6901 ttatgatcgt tttgttttaa aggaggaagt atcggcagta gacgaatgag tacgatgtaa
     6961 gcgcaaatct caatagccac aaaaccaata gaaatcaaca gttcttccca ggtcgggaag
     7021 taggcgtaac cgccgcccgg gttgaatgcc accagcgaat aggtcagacg ccaggttgca
     7081 caacctaaca gtgcgctcag tgctgacagg aacagcatgc gggaatcatt acgcagcttc
     7141 gccacacgca gaacgaccag cgggaagagc atcagcagga cttcaatcca gaacatcacg
     7201 gagtagaagt caccggcaaa cgctaacgac agcttgtcgc gatagataag ctcgccaaag
     7261 cgcagcacga tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt aacaaacaga
     7321 ctcttttcat ccggaccgtt gccacgcaga cccgcctgca ccagcgaacc ttcaaagatg
     7381 acaatcgaga agcccatgat gaacgccgtc agcagcgaga acagcggcaa catttcatag
     7441 ctctgccaca acggatgcac cttgtagccc gccgagatca tcagcgaccc cattgaagac
     7501 tggtgcatgg tcggcagcag cgcaccgagc gcgatgatga agaacatcac cttgtttagt
     7561 cgctgtagcg acaccttcca ccccagacgt tcaaacagtg ccggagcaaa ctccagtgcc
     7621 atcacgccga tgtagatggt catacagacc gccgtctcga acagtaccga gttcacgttg
     7681 aagtgacccg gaatgtagaa gtacggcagg ttccagtagc gacccacgtc gatagtgatc
     7741 gacaaaccac ccagtgagta accaaacaga ctcgccaaca gcgccggacg caccagcgga
     7801 tggtattgcc cacggttaaa gacgtatacc gcccacgcca gcgcccagcc gccacaggca
     7861 aagccggtgc caatcagcag gtcaaacgcg atccacacgc cccacgggaa gccgccgttc
     7921 aggtcagaga cagagcccag accgaacacc agacgcttca caatcaggag catacagatg
     7981 acgattaacg gtccaaaaat catgaccggt ttactaatga ttttgccgcc cagcggttgt
     8041 ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt tgcgacgaac
     8101 cagcacggtt aagcccgcca gcacagccag tggtagcatc atgcctttat acagggtgtg
     8161 ttgaatattt tcggaacgcg caccggtaga aagatcgtcc agtttcggca ggtcgagatt
     8221 ttcataaggc acacccgtca gtaccagaac ctgagtaccg ccgccctctt tctcgccgta
     8281 cagatgcgga taatattgcg gcaccgtatg caggtaagtg tcgccagatt tcagcgtctg
     8341 acgcggatag tggtattcgc tgccaggctt cagcgccaga cgttttttcg cctccgccat
     8401 cagctcttca cgcgtaccga aaatcaccgc gcccgccggg cacacttcta cgcagccagg
     8461 cagaccgcct ttatcgagac gttccacacc tttctggttg cacagctcgc acttatgcag
     8521 cgcaccaaac gggttgttgt agtcgtactt cggcacgttg tacggacagg cgaccatgca
     8581 gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca atgccggttt tcggatcttt
     8641 tttcagtgca gagaccgggc acacagagac acagttcgga tcgacgcagt gcatacactg
     8701 tttcttaatg tacgcgtagc cgttctcctc ctggtctttg ttgccccctg tgccgctggt
     8761 ccacacctga atgatgttat tggtatacgg cgacagtttg tcgttgttcg accaggtctg
     8821 ttccccttgc gggttacgtt cagggaagtt gatatcctga cacttggtga cgcaagcctg
     8881 gcagcctacg cacaaggtcg agtcgtacaa catccccagc gatcccggaa ttggcgggcg
     8941 gttttcagca gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg ccccgcagga
     9001 ggctgcttta ataaaattac gtctgttcac ggttattctc cccgtgagtc agcgttatct
     9061 ttcttttgct gacgacccag ttcacgcacc gccatcacgc tgacaccggc aaccagccca
     9121 accacaccgc cgagcaaacc aatagcgcct gcagagacgt tgccgccctc tttagcgtta
     9181 acatccggtt tctgtgaacg cggagtctga ttttcgacgt tggcaagctg atggatgcct
     9241 ttatggaagc cgataccttc ttcgttacag ccatagcatg ggtgaccaat cgctaccggc
     9301 cacacgccgc caacatcgca gaattgcagc gttgagcagt tgccgtaagt ttctggccct
     9361 ttacagccga ggtggtacag gcaccagcct tcacggtggc cttcatcacc gaactctttg
     9421 gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat cagacggcca
     9481 taggcgaagg tcggacggtt tttgtcatcc agtttcggcg gtttgccgta agtgatgatg
     9541 tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat attgataacg
     9601 gttttgcctg ccagaacttc ttgcaggctg actgcgccag ttgggttaac tccagctgcg
     9661 gcaacaccgc cccacgcaga gcaggaaccg atagcgataa tggctgctgc gccttctgcc
     9721 gctttgcgga tgtgatccac aatcggctca ccagcaacca tgcaataaat accgttatct
     9781 tttaatggga tggaaccatc cacaaccaac acatactgcc ctttgtactt ctcaagtgcg
     9841 ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc gtgatactcc
     9901 agagagatag tctccagcac gaggttttct accgttggat gcgttgcacg aagcagagat
     9961 tccgtacaac cggtgcactc ctgcgcgcca atccagataa ctggcggacg ctgcgggtta
    10021 gtaaccgatt cggccatctc tgcagcggct ttgctactta accccatggt ggcggctaat
    10081 gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt gagaatggat gagggtgtta
    10141 tctccagtca tttatagtta ttccgttgcg aagacctggc atatattttg cctcaatcgc
    10201 aaaatcaata atgcgatcga tgcgccattt accacacatt tattatggtt atcgatatca
    10261 tgatactgcg gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt aattaaagca
    10321 agataatacg tatgtttgat caattttcga ggcgataacc ggccataaaa aagcgcaacg
    10381 tagaacagga attattctca cgaaagaaat ccattaccat ctctttatcc agtccatagc
    10441 ggcgcgtcag aatacccgct tcccaggcgc taagctggcg atctccggtc ttttcgtaca
    10501 tccggtgtga gtagcctaat acaaaaccgc gtttataatc ggcacagaat tttgtcacat
    10561 taaccgcact gtcggcatgc gttgctttta atcccgccat taagccttta ccaaaatggt
    10621 tattcatcgt tcacctcatt cgctatatat cgccatatat agcgatattt taggcaaaga
    10681 acgacttttg cgcacgctat tttttcatgc ttgtttaaga cgtaatcccg taagagataa
    10741 acctatcagg agagatattt atcgaaccat ttcagtgtcc tttgccaggc aagatcggcg
    10801 gcagatttgt cataacgggg cgtggaatca ttatggaatc cgtgattaac ccccggatag
    10861 atatacgcct cataaacctt attattggct ttcaacgccg cctcgtaagc aggccagccc
    10921 tcgttgattc gggtgtccag ttccgcgaag tggagtagta aaggcgcttc aatcttcgcc
    10981 acatcggcag tgggtgcctg acgaccataa aacggcaccg cgcaggccag ttccggatac
    11041 gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa atccggtaat acccactttg
    11101 cctgtcgctt gcggatagcg ttgcataaac tcaattgcgg caaagaaatc attcatcagt
    11161 ttggttggat caacctgctg ttgcagctca cgacctttat catcattacc cggataacct
    11221 ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgc cactcgccgt
    11281 gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac ggctggcgtt
    11341 ttgccgctca tccttgcggg tttcaccaga taaccccgta cctcgccgtg accatttggc
    11401 gaaggatacg tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgcc
    11461 agcgcataat ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca
    11521 tacttcgccg caaggttgag gaactcacgt ttcgagattt tcccgtgagc gtaatagtcg
    11581 tagtaatcca acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttgcactc
    11641 ctcaactggt gtttttttaa gcaaagcata agcacgtgtt ttcgcccagt ttttcgtcac
    11701 tctgtgagcc agactacggg atacgcgctg gcgaatcgct aaactagaaa cattgtttcg
    11761 aaattgaacg gtggaaagga gaggtcatgg tctggttagc gaatcccgaa cgttacgggc
    11821 agatgcaata ccgctattgc ggaaaaagcg gtttacgcct gcccgcgtta tcgctcggtt
    11881 tatggcacaa tttcggtcac gttaacgcgc tggaatcaca gcgtgcgatc ctgcgtaaag
    11941 cgtttgattt gggcattacg cactttgatt tagccaacaa ttacgggccg cctccaggaa
    12001 gcgcagaaga gaactttggt cgcctgctgc gggaggattt tgccgcttat cgcgatgaac
    12061 tgattatctc taccaaggct ggctacgata tgtggcccgg cccttacggc tctggcggtt
    12121 cacgtaaata tctgctcgcc agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg
    12181 tcgatatctt ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg
    12241 cgctggctca tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc tcttactcgc
    12301 cagagcggac gcaaaaaatg gtcgagttgc tgcacgagtg gaaaattccg ctgttaattc
    12361 atcaaccttc gtacaattta ctgaaccgct gggtggataa aagcggcctg ctggataccc
    12421 tgcaaaataa cggcgtgggc tgtattgcct ttactcctct ggctcaggga ttgctgaccg
    12481 gaaaatatct caacggcatt cctgaagatt cacggatgca tcgtgaaggg aataaagttc
    12541 gtggcctgac gccgaaaatg ctcaccgaag ccaacctcaa cagcctgcgc ttattgaatg
    12601 aaatggcaca gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag
    12661 atgatcgcgt gacgtcggta ttgattggtg ccagccgcgc ggagcaactt gaggagaacg
    12721 tgcaggcgct gaataatctg acatttagca ccgaggagct ggcgcagatt gatcagcata
    12781 tcgccgatgg cgagctgaat ctgtggcagg cgtcgtccga taaatgacct gttaataacg
    12841 ggcgcgcgaa ccgcgcccga atcagatcag tgattatgac gagtcagtcg gtcaagatag
    12901 cccatcacaa atgcagagag cacaaacgtc agatggataa tgacgtacca catcagtttg
    12961 ttatcaggga catttttcgc atccataaag acgcgcagta agtgaatgga agaaattgcc
    13021 acaatcgacg ctgctacttt gtttttcagc gacgttgcgt ccattttccc cagccagttc
    13081 agcttctctt tgttctcaga gatatccagc tgtgagacga aattctcata accggaaaac
    13141 atcaccatca ccagcaaacc gccaaccagc gtcatatcca ccagcgacag caacacgagg
    13201 atcaaatctg attccgccat cgagaagata ttcggcagta cgtgaataat ctcctggaag
    13261 aacttcagcg ccagggcaac taacgccagc gaaaggccaa agtacacggg ggcaagcagc
    13321 cagcgagaag catacattgc attttcaaga aaacgttcca tacattcctg tcgtgtagtt
    13381 aaaacgggca acagtatatc gcaattgagt aaacagcacg caacagcaaa tcaacatata
    13441 cgttaaacct ttgtcgcata attctgaggt ttagggatct tcctggttcc cttatcaacg
    13501 catcgagaac gtctaccctt aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat
    13561 ttaaaagacc cgaccacgca gtattacact ggtgaatatc ccaaacagaa acaaccgacg
    13621 ccaggcatcc aggcgaagat gacaccggta ccggattgcg gcgagaaaac ctatgttggt
    13681 agcggtcgcc tgaaagatcg taaagcactg gtgacagggg gcgattccgg aataggtcgc
    13741 gctgccgcca tcgcttacgc gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg
    13801 gaagaagaag acgctcagga tgtgaaaaag atcattgaag aatgcggacg caaagccgtt
    13861 ctgctgccag gcgatttaag cgatgagaaa tttgcccgtt cgctggttca cgaagcgcac
    13921 aaggcgttag gcgggctgga tattatggcg ctggtcgccg ggaaacaggt tgccattccg
    13981 gatattgcag acctcaccag cgaacagttt caaaagacct ttgccattaa cgttttcgcg
    14041 ctgttctggc taacccagga agcgatcccc ctgctaccga aaggtgcaag tatcatcacc
    14101 acttcgtcaa tccaggcata ccagccaagt ccgcatttac tggactatgc ggctacgaag
    14161 gcggcgattc tgaactacag ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg
    14221 gtgaatattg tcgcgccagg cccgatctgg acagcactgc aaatttccgg cggacaaacg
    14281 caggataaga tcccgcagtt tggtcagcaa acgccgatga aacgtgcggg gcaaccggcg
    14341 gaactggccc ctgtatatgt ttatctggca agtcaggagt cgagctacgt caccgcagaa
    14401 gtgcacggcg tgtgcggcgg cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca
    14461 accgggcatc attgtgaatt cactcttacc tgatgtggcg aaaacgcctt atccgcctac
    14521 aaacgcatga agattcaaca tgttgcaata gattttgtag gcctgataag cgtagcgcat
    14581 caggcattga gcacctaatg ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca
    14641 cgaagattca acatgttgca atagattttg taggcctgat gagcgtagcg catcaggcaa
    14701 ttctacttta cttcgctttg gcggtttctt cgccgaccag acctatcttc aggtaacccg
    14761 cctgatgcag cgtatccatt accttcatca acgtctcgta atcgacggtt ttgtccgctc
    14821 ggaagaagat ggtggtgtct ttcttgcctt cggttaacgc attcaacgcc gtaatcattg
    14881 tttcatcggt gaccggatcg ttaccgataa acatcgagtt gtctgccttc accgacagat
    14941 aaaccggttt ttccgggcgc ggctgcggcg tgctggtaga agcaggcaag ttcaccttca
    15001 catctaccgt cgctaacggt gccgccacca taaagataat cagcagaacc aacatcacgt
    15061 cgataaacgg cgtcacgttg atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa
    15121 gatgcattgc catcggatat tatcctgcgc gtaatttttg tgcgacacga accggatgcg
    15181 cagcggcgct ggcttccaga tccaggtcac ggctttgcag caacaatacc tgcgctgcaa
    15241 catcacccag catcgcttta aagccgccaa tctggcgtgc aaatacgtta tagataacga
    15301 ccgcaggaat cgctgccacg aggccgattg ccgttgctaa cagagcttct gcgatacccg
    15361 gcgcaacgac tgccaggtta gtggtctgcg tttgcgcgat accaataaag ctgttcatga
    15421 tgccccagac cgtaccaaac agaccaacga acggagaaat cgcgccgatg gttgccagat
    15481 agccgttacc gcggcccatt tgacgaccca ctgcggcgac ccgacgctcc agacggaagc
    15541 tggtacgttc tttaatacct tcgttatcgt cgctgccttc tgacagttcc agctcattct
    15601 gcgcttcatt gagcaaatgc aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt
    15661 tggcctggtt taaggaacgc gcttcagcca gcagttgctg ctcgcgctta aggcgacgct
    15721 tctgattgaa gaactctacg ctcttactga agaagattgc ccaggtgact acggaggcca
    15781 aaataagccc aatcatcacg cacttaacga caatatcggc gtgctgatac ataccccaga
    15841 cggaaaggtc cgtctgcatt aaattattac ccactgtgta tctccaggac gcaagtcaca
    15901 aaatctgcgc ataataatat caaaacgacg tcgaattgat agtcgttctc attactattt
    15961 gcatactgcc gtacccttgc tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc
    16021 acgccatttg cgaaaatttt ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt
    16081 catgctagtt tagacatcca gacgtataaa aacaggaatc ccgagatggc ggacaaaaag
    16141 cttgatactc aactggtgaa tgcaggacgc agcaaaaaat acactctcgg cgcggtaaat
    16201 agcgtgattc agcgcgcttc ttcgctggtc tttgacagtg tggaagccaa aaagcacgcg
    16261 acgcgcaatc gcgccaatgg cgagttgttc tatggacggc gcggaacgtt aacccatttc
    16321 tccttacaac aagcgatgtg tgaactggaa ggtggcgcag gctgcgtgct atttccctgc
    16381 ggggcggcag cggttgctaa ttcgattctt gcttttgtcg aacagggcga tcatgtgttg
    16441 atgaccaaca ccgcctatga accgagtcag gatttctgta gcaaaatcct cagcaaactg
    16501 ggcgtaacga catcgtggtt tgatccgctg attggtgccg atatcgttaa gcatctgcag
    16561 ccaaacacta aaatcgtgtt tctggaatcg ccaggctcca tcaccatgga agtccacgac
    16621 gttccggcga ttgttgccgc cgtgcgcagt gtggtcccgg atgccatcat tatgatcgac
    16681 aacacctggg cagccggtgt gctgtttaag gcgctggatt ttggcatcga tgtttctatt
    16741 caggccgcca ccaaatatct ggttgggcat tcagatgcga tgattggcac tgccgtgtgc
    16801 aatgcccgtt gctgggagca gctacgggaa aacgcctatc tgatgggcca gatggtcgat
    16861 gccgataccg cctatataac cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa
    16921 catcatgaaa gcagtctgaa agtggctgaa tggctggcag aacatccgca agttgcgcga
    16981 gttaaccacc ctgctctgcc tggcagtaaa ggccacgaat tttggaaacg agactttaca
    17041 ggtagcagcg ggctattttc ctttgtgctt aagaaaaaac tcagtaatga agagctggcg
    17101 aactatctgg ataacttcag tttattcagc atggcctact cgtggggcgg gtatgaatcg
    17161 ttgatcctgg cgaatcaacc agaacatatc gccgccattc gcccacaagg cgagatcgat
    17221 tttagcggga ccttgattcg cctgcatatt ggtctggaag atgtcgacga tctgattgcc
    17281 gatctggacg ccggttttgc gcgaattgta taacattgcc acttttggac aattttgcag
    17341 acatttaatt gtgaaaagtc ttaaattgtt gcgtccggga tcaaggcgtc ccggacgaat
    17401 caggagtaca ataggcagat aaaggcttaa acgctgttcc acaggaaagt ccatggctgt
    17461 tattcaagat atcatcgctg cgctctggca acacgacttt gccgcgctgg cggatcctca
    17521 tattgttagc gttgtttact ttgtcatgtt tgccacgctg tttttagaaa acggcctgct
    17581 gcccgcctca tttttgccag gcgacagctt gttgatactg gcaggcgcat tgattgccca
    17641 gggggttatg gattttctgc ctacgattgc gattctgacc gccgcagcaa gtctgggctg
    17701 ctggctaagt tatattcagg ggcgctggtt agggaatacc aaaacggtga aaggctggct
    17761 ggcacagctt cctgctaaat atcaccagcg cgccacctgc atgtttgacc gccacggtct
    17821 gctggcgctg ctggctggac gttttcttgc atttgtccgt acgctgctgc caaccatggc
    17881 gggaatttcc ggtctgccaa accgccgctt ccagtttttc aactggttaa gtggattgct
    17941 gtgggtcagc gtggtaacca gttttggcta tgccttaagt atgattccgt tcgttaaacg
    18001 ccatgaagat caggtaatga cgttcctgat gatcctgcca attgccttgt taaccgctgg
    18061 cttgttaggc acgctgtttg tggtgattaa aaaaaaatac tgtaacgcct gacgattttc
    18121 cccgttcccg gctgctgtac cgggaacgta tttaattccc ctgcatcgcc cgcattcttg
    18181 ccgcatcttc ccccggcgtc acaccgaagt aacgtttaaa ctcacggcta aattgcgatg
    18241 cgctttcata gccgacgcgc atcgctgctg cgctggcctt catgccgtca tggatgatca
    18301 tcatccgcgc cttatgcaga cggtaattct tcaaatactg caacggcgag gtgctggtga
    18361 cagacttaaa attatggtgg aacgccgata cgctcatgtt ggcttctgcc gccagttgct
    18421 cgacgctcag gttttcggtg tatttattct caatccgttt cagcacgcgg ctaatcagac
    18481 tgaagtgagt ctggcgactg accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca
    18541 cgtagtacag aatttcgcgg atgatctgtt tgccgagaat acgcgcatcc agtggtcgct
    18601 ccatcacgtc gagtaaccgc tccgccgcgc ataaaatctc ttctgataac gtggcggagt
    18661 taatcccgct ggctgccatc gacggctgga aatgctcatc ttcgccaatg tccatcaaca
    18721 gttcctgtaa ctgcaaaata tcgacattga gacgcaaccc tgccagcggc acctctgacg
    18781 tggcataggt ttcgcactca aacggcaacg gcaccgtcag cagcaggtat tcattggcat
    18841 cataacgaaa cacgcgttca ttgatataac cgattttatg cccggaaaag agaattatga
    18901 tgccaggctc gtacatcacc ggtgtacgtg cgaaaggtgt ctcgccatac aacaaacgca
    18961 catcgggcaa cagttctgac aaactatttt ctttattttt cagtttatta actttatccg
    19021 ccagcaagcg gcaaatctct tcacgtttca tatcgcgtaa tttcttagga ataatgcggc
    19081 aatttgattg tgcgcaattt tgtagcattt ctccagcact ctggagaaat aggcaagaca
    19141 ttggcagaaa tgagcattga gagccagggc gctggcgatc acaatgaaaa acatcaggca
    19201 gatcgttctc tgccctcata ttggcccagc aaagggagca agtaatgaac aactttaatc
    19261 tgcacacccc aacccgcatt ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa
    19321 ttcctcacga tgctcgcgta ttgattacct acggcggcgg cagcgtgaaa aaaaccggcg
    19381 ttctcgatca agttctggat gccctgaaag gcatggacgt gctggaattt ggcggtattg
    19441 agccaaaccc ggcttatgaa acgctgatga acgccgtgaa actggttcgc gaacagaaag
    19501 tgactttcct gctggcggtt ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg
    19561 cagcggctaa ctatccggaa aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag
    19621 agattaaaag cgccatcccg atgggctgtg tgctgacgct gccagcaacc ggttcagaat
    19681 ccaacgcagg cgcggtgatc tcccgtaaaa ccacaggcga caagcaggcg ttccattctg
    19741 cccatgttca gccggtattt gccgtgctcg atccggttta tacctacacc ctgccgccgc
    19801 gtcaggtggc taacggcgta gtggacgcct ttgtacacac cgtggaacag tatgttacca
    19861 aaccggttga tgccaaaatt caggaccgtt tcgcagaagg cattttgctg acgctaatcg
    19921 aagatggtcc gaaagccctg aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt
    19981 gggcggcgac tcaggcgctg aacggtttga ttggcgctgg cgtaccgcag gactgggcaa
    20041 cgcatatgct gggccacgaa ctgactgcga tgcacggtct ggatcacgcg caaacactgg
    20101 ctatcgtcct gcctgcactg tggaatgaaa aacgcgatac caagcgcgct aagctgctgc
    20161 aatatgctga acgcgtctgg aacatcactg aaggttccga tgatgagcgt attgacgccg
    20221 cgattgccgc aacccgcaat ttctttgagc aattaggcgt gccgacccac ctctccgact
    20281 acggtctgga cggcagctcc atcccggctt tgctgaaaaa actggaagag cacggcatga
    20341 cccaactggg cgaaaatcat gacattacgt tggatgtcag ccgccgtata tacgaagccg
    20401 cccgctaagc tttttacgcc tcaaactttc gttttcgggc atttcgtcca gacttaagtt
    20461 cacaacacct caccggagcc tgctccggtg agttcatata aaggaggaac gtatggctaa
    20521 tccaaccgtt attaagctac aggatggcaa tgtcatgccc cagctgggac tgggcgtctg
    20581 gcaagcaagt aatgaggaag taatcaccgc cattcaaaaa gcgttagaag tgggttatcg
    20641 ctcgattgat accgccgcgg cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa
    20701 tgcctcagtc aacagagaag aactgttcat caccactaag ctgtggaacg acgaccacaa
    20761 gcgcccccgc gaagccctgc tcgacagcct gaaaaaactc cagcttgatt atatcgacct
    20821 ctacttaatg cactggcccg ttcccgctat cgaccattat gtcgaagcat ggaaaggcat
    20881 gatcgaattg caaaaagagg gattaatcaa aagcatcggc gtgtgcaact tccagatcca
    20941 tcacctgcaa cgcctgattg atgaaactgg cgtgacgcct gtgataaacc agatcgaact
    21001 tcatccgctg atgcaacaac gccagctaca cgcctggaac gcgacacaca aaatccagac
    21061 cgaatcctgg agcccattag cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg
    21121 cgatctggca gataaatacg gcaaaacccc ggcgcagatt gttatccgct ggcatctgga
    21181 tagcggcctg gtggtgatcc cgaaatcggt cacaccttca cgtattgccg aaaactttga
    21241 tgtctgggat ttccgtctcg acaaagacga actcggcgaa attgcaaaac tcgatcaggg
    21301 caagcgtctc ggtcccgatc ctgaccagtt cggcggctaa catgcaaatt ctcccggtgg
    21361 cggtaatgtt ccgctaccgg acttttcaga aatcatttat tcccctcgcg tcccgcccgt
    21421 tgttactctt ccttgttcag gaatgccaaa tataaggaca tcatcatgca gagccggaag
    21481 ctcttaaaag aacaactcat ctatatccgg gataaacgca acggagaggt gaaaaacaga
    21541 tgaaaataat acttctgttt ttagcagccc tggcaagttt taccgtacac gcacagcccc
    21601 cctcacagac cgtagaacaa acagtccggc atatttatca gaactataaa tcagatgcca
    21661 ctgcccctta ttttggtgaa accggagagc gggcgataac ttctgcgcgt attcaacagg
    21721 cgcttaccct gaacgacaat cttacgctgc cgggcaatat tggctggctg gattatgatc
    21781 cggtttgtga ttgtcaggat tttggcgatc tggtgctaga aagcgttgcg ataacccaaa
    21841 ctgacgccga tcatgccgat gccgttgtgc gctttcgtat ctttaaagat gataaagaaa
    21901 agaccacgca gacactgaaa atggtggcgg aaaatggtcg ttgggtcatt gacgatattg
    21961 tcagcaatca tggcagcgtc ttacaagcag ttaatagcga gaatgaaaaa acgctggccg
    22021 ctttagcttc gttgcaaaaa gaacagccgg aagcctttgt tgccgaactc tttgaacata
    22081 ttgctgatta tagctggccg tggacgtggg tggtttccga ctcttaccgc caggcggtta
    22141 atgccttcta taaaaccacc ttcaagacgg ccaataatcc cgatgaagat atgcaaatag
    22201 aacggcaatt tatttacgac aatccgatct gttttggcga agagtcgcta ttttcacgcg
    22261 ttgatgaaat tcgagtcctg gagaaaaccg ccgattccgc ccgcattcat gttcgtttta
    22321 cgctgaccaa tggcaacaac gaagagcaag aactggtttt acagcggcgc gaaggcaagt
    22381 gggaaatcgc tgattttatc cgcccgaaca gcggcagcct acttaagcag attgaggcaa
    22441 aaactgccgc cagattaaag caatgagctg aattaaataa caattagccg gaacaataaa
    22501 taaaagggaa cactatatga aaacgatttt caccgtggga gctgttgttc tggcaacctg
    22561 cttgctcagt ggctgcgtca atgagcaaaa ggtcaatcag ctggcgagca atgtgcaaac
    22621 attaaatgcc aaaatcgccc ggcttgagca ggatatgaaa gcactacgcc cacaaatcta
    22681 tgctgccaaa tccgaagcta acagagccaa tacgcgtctt gatgctcagg actattttga
    22741 ttgcctgcgc tgcttgcgta tgtacgcaga atgataaaaa aatccccggt agcgtgtcag
    22801 ttgccgggga tattttttaa cgtccaaccg ccgctttagg ccgtttcttc gcaccagcat
    22861 tcaccggacg agattgcgta gacgacgctt tttttgccgt agcaggcgtc tgacgctgga
    22921 tcgccatcgg cgtatgtttc gtcaacgccg gacgggtatt gcggttctgg cgacgagctt
    22981 cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat
    23041 gctttttgcc catcgcttcc agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt
    23101 aacgcaacaa cgctttatgc aaacgacgct gtttgtcgcc cttcggtacg aagacgtctt
    23161 cgctcttata accaatcttc gccagcgggt ttttcccggt gtaatacatg gtggttgagt
    23221 tcgccagcgg cgacggatag aagttctgca cctggtcgag gcggaagcgg tgctttttca
    23281 gccacagcgc cagattcacc atatcttcat cacgcgtacc ggggtgcgcg gagatgaaat
    23341 acgggatcag atactgctct ttacctgcct gtttcgagta agtatcgaac agctctttaa
    23401 agcggtcata gctgcccatg cccggcttca tcatcttcga taacggccct tcttcggtat
    23461 gttccggggc aatcttcaga taaccgccga cgtgatgggt cgccagctct ttgatatagc
    23521 gaggatcttc cacggcgatg tcataacgta cgcctgaggc gatgaggatc tttttaatgc
    23581 ctttcagatc acgcgcacgg cgatagaggt tgatcgtagg ttcgtggttc gtgtccatgt
    23641 gcggacaaat atccggataa acgcacgaca aacggcgaca agtttgttca gcgcgtggcg
    23701 atttgcagcg caacatatac atgttggcag ttggcccacc aagatcggaa atcacgcccg
    23761 taaaacctgg aacggtgtcg cggatcgctt cgatctcatt aatgatcgaa tcttcggaac
    23821 ggctctgaat aatgcgccct tcgtgctcgg tgatagaaca gaaagagcag ccgccaaagc
    23881 agccacgcat aatgttgacc gaaaaacgga tcatttcgta agccggaata cgggcattgc
    23941 cataggccgg atgtggcacg cgcttgtatg gcagcgcaaa aacgctgtcc atctcttcgg
    24001 tagaaagcgg aatagcaggc gggttgatcc acacatagcg gtcgccgtgt ttttgcatca
    24061 atgcgcgggc acagcctggg ttggtttcgt ggtgcagaat acgcgaagca tgggcgtaca
    24121 gcactttatc gcccttcact ttctcgaaag aaggcagcaa cacgtaggtt ttttcccacg
    24181 gtttcgggcg cggtggctgc acggttacgg ctttggcttc ctgctttttc ggtgccaccg
    24241 gtttgttatc cgcgcacggc aaatcttcac catacggatg cgggattggg tcgatttttc
    24301 caggggtatc aagacgggtg gaatccacgc cgctccagcc aggcagcgcc tctttcacga
    24361 taatcgcggt attacgcaca tcgcggattt cactaatcgg ctcgcccatc gccagacggt
    24421 gcgccacctc caccagcgga cgctcaccgt taccaaacat cagcatgtcg gctttcgaat
    24481 ccaccagcac ggaacggcgc acggtatcgg accagtaatc ataatgtgcg gtacggcgca
    24541 gactagcctc aataccgccg aggatcaccg gtacatcttt ccacgcctct ttacaacgct
    24601 gggtataaac cagtgtggcg cgatccgggc gcttacccgc gacgttatcc ggcgtgtagg
    24661 catcgtcatg acgtaaacgg cgatcggcgg tataacggtt gatcatcgaa tccatgttgc
    24721 cagcagtaac accgaaaaac agattcggtt tacccagacg catgaaatcg tctttgctgc
    24781 tccagtctgg ctgggcgatg atcccgacgc gaaagccctg tgcttccagc atacgaccgc
    24841 aaatcgccat cccgaagctt gggtgatcga catacgcgtc gccagtaacc aaaatgatgt
    24901 cgcagctatc ccagccaagt tgatccatct cttcacgaga catcggcaaa aacggtgccg
    24961 gtccaaaaca ggcggcccag tactgcggcc aggagaacag gtcgcgatcc ggttggatca
    25021 gggagataga gctcattttg cttccagaaa tgataaaaaa ataatcaaag gccggggatt
    25081 ataagccgga acgaaagaga aatcgaaaag tattccatac tcgccctcct cgggcgagta
    25141 tgaagattac ggtaccggat tgactaacag ttgcccaatc gagccacggt ccgccatttc
    25201 cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag gaaggctgac cgaaataaac
    25261 aagcagctcc acttgtccgt caacccaaac ggtatctttc cagcctctgt cttccgggaa
    25321 cggcatcgcg ccattcacgt tacggatctg gaacattacg ccttcaatat ggaacgcttg
    25381 cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat
    25441 acggttgacg tcccacagct gtccattaat acccgggtca tcacccagac tgatatcgcg
    25501 actgcgaatt ggcgaaccag ccatgatttc agttggcagc aagcgcatcg gaagactgtc
    25561 tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc accagggtag aaaccagaat
    25621 actggatggc tcaaagaagc cacgaatacg atcaacaatg ctcgccgctt cgccacaggt
    25681 gatcgacact tcatcgccgt tgctcatatc caccagaatc tcgcggcgct cgcccggtgc
    25741 cagcgaaagt tgcttcaccg atacaggagc aggcaggaat ccctgatcgc cagaaatcac
    25801 atgtaacggg cgaccatcgc tcatctgtag ttgatagcga cgagagttcg acgcgttcag
    25861 cagtcgcaag cgcacccagc cacgcgagac ttcaacgtac gggctttgta caccgttaac
    25921 cagcagcgta tcaccaacaa agccgccgct tcccggttcg ttgtattctg acgtaccaaa
    25981 gttatccagc cgtttatcct ggataatgac cggaaaatca tccacaccat aatggttggg
    26041 gataggcagc gacttgctga cttcatcttc caccagccac attccggcaa ggccgttata
    26101 gacctgctga gccgtgcggt tgggagtatt ggcgtgatac cacagagtag ctgcgttctg
    26161 gcgaatgggc agtacgggtg cccagtcagc gtttggcgac atcatccgtg ccggaccgcc
    26221 catcagcggg cctggtacct gtagcccggc caccgtcatt gagacatttt ctgtcaggcg
    26281 gttgctgtaa ataagcttaa catcgtcgcc cttccagacg cggatagtcg gccccaggta
    26341 acgaccattg attccccaga ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg
    26401 ttgtacagtc ataaacagcg gttgcccacg gcgagattca agtagcggcg gaacgggtag
    26461 cggttgctgt tgcccggctg cgctggcttt caggggaaca gcgcctgcac aaagtgcaat
    26521 ccccgatgcc tgaatgaact gacgccgact gagtgacata tttgctccat gtaaaactgg
    26581 ctaaagtaat accgtgcagg aaagtgttcc ccgcaaaaat acagattgcc gacttaaact
    26641 tttccggcgg cttcgcgttc tgcgacttct ttatcgagct cggcgatttt ttgttccatt
    26701 atcgaacgac aatgggcagc cagctcacga acctgatctt tgccatactg actgacgtca
    26761 attggcggca gcatttcgac aatcaccaga ccgttgtgca gtcgattaag attaatctta
    26821 ttcgaagttg tagagacgca cacgggaata atcgggacgc ccgccgcaat tgccgcgtga
    26881 aatgctccag tcttgaacgg tagcaggccg cgaccacggc tgcgggttcc ttccgggaac
    26941 atccagatgg aaatacggcg ttttttgaag tgattcacga cttccgcaat ggtgccgtga
    27001 gctttagtgc gattgtttct gtcgatcaat aagttgccgg ttaaccagta caactgcccg
    27061 aagaagggga tccacagcaa gctcttttta cctaccgtca ccgtcggcgg ttgcacgatg
    27121 ttcgatgctg tcaccatgtc atagttgttc tggtggttag cgatatagat agcattgccg
    27181 tagctttcag cgtctgtagg tttacggcac tcaactttca ggccaaacag cggcgcaaga
    27241 cggccaaaca tatgcccaaa ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag
    27301 taaatggagc cgaatacaca gactaagatg ctgtaaatca cggtaataat aagacgaaag
    27361 atatatagca taacaccctc tgaaggtctg agagactggc attgtacgtc acctgaaatc
    27421 aggttagggc attgttaaaa gccgctctct ggaaaagcgt attgttaatc gcaacgcatg
    27481 aataaacaac ggtttcaagg gaatttttat cgtttccggg ggttgaatac gctgccggat
    27541 gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga ggaaacagcg ccctccccgg
    27601 catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc tcaacacgat
    27661 cgatacactg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca gtgactttct
    27721 gtagctcttc cggacgcagt ttaattttgc gtttcccaac atgaatggtc agcgtgcttt
    27781 gcggcggcag aacgtacagt tgcgccagac catcttctcc acgtgcggct tctgccgatg
    27841 gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatcg cttaccggga
    27901 acatcaacat acggcctgcc tgagtgattg ccagcagcat atcggaagca tcttcaatca
    27961 ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcctta cctgcacggt
    28021 tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg gaagccatca
    28081 gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgcccca ggcggcaacg
    28141 ttaatttgcc ggtgagcggc tcgccctgac cacgcgccga cggcagcgta atcgggtcaa
    28201 tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc ttacctttca
    28261 ccgccgcttt gaagctatca cccgctttat aattcaggcc cggcgcgtcg atatcatggc
    28321 ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt tcagacggca
    28381 gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat
    28441 cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt cagcaggtta ttcattttac
    28501 gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagttca ctctgctcac
    28561 cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg aggatcgctt
    28621 ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt tcatcttcat
    28681 tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc caccagcaaa ccttcgagga
    28741 tatgcaggcg cttgaggact ttctccagac gatagttcag tcggcggcgc acggtatcgc
    28801 ggcggaacac cagccattcg gagaggattt ccagcaggtt tttcaccgcc ggacgaccat
    28861 ccagaccgat catattaagg ttaatacgat agctcttttc cagatcggtg gtagcgaaga
    28921 ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc accaggcggg
    28981 tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc tttttgttgc
    29041 gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc ggcaatgcgc
    29101 tgataaccac cgcgccatct tctttcttcc acaccgcgcg catacgcact gaaccacgtc
    29161 cgttctcgta gattttacgg atctcggcgc gcgaagtgat aatttccgct tcagtcggat
    29221 aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc tggtcgatta
    29281 atgcgattgc cgcctgagcc acttcacgca ggttatgcgg tggaatatcg gtcgccatgc
    29341 cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgggca ggtagcattt
    29401 tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc tgccccagct
    29461 cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc attgccgcga
    29521 acgatttcgg atcgtccggc gcgccccagt tcccctgacc atcaaccagc ggataacggt
    29581 aagagaacgg ttgcgccatc aggaccatcg cttcataaca ggcgatatcg ccgtgcggat
    29641 ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg gcgctggcat
    29701 tcaggcccag ttcagacatc gcatacacaa tgcggcgctg aacaggtttc agaccatcac
    29761 caataaacgg caacgcacgg tccatgatca cgtacatgga gtagtttaag taggcgtttt
    29821 ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct gattcctcaa
    29881 cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat ctgccgcgtc
    29941 gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccgacggc gataaaggca
    30001 tcaatgtgcc ttgatataca actctttgct gtagacataa tcctggggat tatgcagcgg
    30061 aaaaccgcca acgtagggtt taagcagttt gattaacggc tgatagtaga taggaatcag
    30121 cggtgcctgt tggttgatga tcacttctgc ctgctgatat aacgcattac gctttgtcgc
    30181 atcagtgatc tgcgtggcct ggtttagtaa ggcgtcatac tgcgcatttt tccagtgacc
    30241 gacgttttct tcactatcgc ttttgagcgt gttcaggaag ctggaagcat cattgtacgt
    30301 cgcatcccac gactgccgag acagcatgaa atcaccggct cgtcgggcat caagataggt
    30361 tttccactcc attgtgcgca gcgtcacctg tgcacccagc cattttttcc attcggaaga
    30421 caacgctatc gcggtctttt catgcagatc gtacttgttg tagaacagct caaagcgtag
    30481 cggatgagag gcgtcgtatc ccgcctgttt cagcaaggct tttgccatcg cgacgcgctc
    30541 actcattggc ttttgcagtt catcgaacgt cgtcgcgcta aagcctttta cctctggcgg
    30601 cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt tgcgcaataa gctgtcgatc
    30661 aaccgtaaga tatagcgccc gacgcactcg cacatcgtta aatggcggtt tctcaaggtt
    30721 gaagttgtaa tattcgctgt tcagacgcgg aataattcgt agctcgccag gcagtgattt
    30781 ttcaatggcg ggaatttgct gcgccggaac ccaggtgaga tcgacctctc ccgcgcgata
    30841 gcggttatag ccggtgaccg aattatctag cgccagatac tcaacctgtt gcaatactgt
    30901 atgttgcgca tcgcggtact ttggattttt gcgtgcagta atcttttcgt taactaccca
    30961 ctgatcaagc acaaaggcac cgttgtaaac catgttctct ggcttactcc agctatcgcc
    31021 atgtttagcg atgacatgat gaggaaccgg gaacagcgtc ggccaggcga gcatcgtcgt
    31081 gaaccaagga accggctgct caagcgtaac ttcaagagta cgatcatccg tcgctttgac
    31141 acccagcgat gtaacatctg ctttacccgc aacaatagct gcggcattgt taatgtgcgc
    31201 ctgtgccaga tagccagcaa aagggcttgc cgttttcggg tcaaccgcgc gctgccagcc
    31261 gaggacaaaa tcctctgccg tcagaggctg accgtcggac cactgcaaac cgctacgcag
    31321 atggaaaata tagcgcttgc cgccgtccag tatctcccag cgttcagcct gagcgggctg
    31381 cacctggcct tcaccgtcca tccataccag accttcaaac agatccagca caatctgcgc
    31441 ggcagtattc tcctccacct tttgcgggtc gagcgtacct gggtcgctat gattgttgta
    31501 acgaaagact tgttgcgggg cgagcggtgt gttggcggga acgtcagcag cgtagagagg
    31561 agccgcactt accaggctga ccagccataa taaatttcgc gtatacatgg tattccttac
    31621 cggagaaatt ataagcccaa caacaatacg cctgtggcat tgttcgctca aacttatcgc
    31681 gagtgatttg gctcacactc cggtaaagaa aaaagcgagt tgcccccgct tttccattaa
    31741 ttaacggatt tcattcaata gttctggatg cttatccaga acacgtaaaa gtttgattgt
    31801 ggaaggatga ggttgggcat tgcctttttc gtaacgcgaa aacgcattta cacctccccc
    31861 aaaaatttcg cttgcctctt tttgggtaag agagagcttt tttcgaacct tcactataaa
    31921 ttcaggtgcc actgtttcgg cattcaccga agcccgaaat gcctttactt gcgccatgaa
    31981 agcatctgac tcttctttat tcatgatgct ctcttcgcaa tggacacaat ataaaccgtg
    32041 gatacctttc aatactgttt ttcgtccacg gaaggtgtat ggaatatctt taatgccaga
    32101 aaccatttct ccctggtggc aaaccggaca tttcatatta cttctcctta aacgagacga
    32161 tcagtacgtc atgaattacc gtaattttaa gataaacctg gcctgtaaca agcctgggtc
    32221 tgtaaacatc ctgccagata gtatgatcag agtaggtggt catgctttta taaaagtcgc
    32281 tctctgataa tccaatgata acattacaca taccatcaaa atccaaacct aactcatctg
    32341 catttaacag tgcactacgt gttgtacgaa cttgcccggc attgacaagt tttttaacct
    32401 gactcaaacg tgtatgtggt gtgcgttttt ccataacccc cgcctccctg ttactttagt
    32461 tataacctaa aaggttaatt acagcaatga aaaagcacct aaaaggttag ttagatgtac
    32521 ggagatagtg accacacaaa acgtattctt taaggaaagt gattgaccat ataagaaagt
    32581 ggcgcattag tagcgccagt ttgaagcagg aatttataag ggaagctgga gtcaggcatc
    32641 acgccaacgg cacataaaca tccgtttcca gtcgctgctc tgtcacccct tcggcaagat
    32701 tggtgtagtg aaacagaatc ggtgctttac gcattttctc gccgcttgca ggcagccagt
    32761 ggcgaatgat gccccatacc gtgtgtgaaa tatcgtccag ctcgccaacg tggcgggcca
    32821 cggcataacg tccaccggta agttcaccat tgctcacacc ataacgatta tcgggaatcg
    32881 gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg gtggcagggt
    32941 cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc gtctctttac
    33001 gccacatgat aaatttcgcc acgctgtagt tgagcaattc agggctacag cggtgcggca
    33061 acatcgccac aggtattgat gggaaatcga taatgtttac atccagtgtc aggtttgtca
    33121 ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa caattacgga
    33181 tttactttgc ggatctgttt gacgtcaatt tcaacagaat tccagtcttt atcgacttca
    33241 ccctgaatct caaccgtatc tttcggcgtc accgtcacgc cgttccagcg tttgtggtcg
    33301 atatcaacat tgatagtacc gctggcatct ttgaacacgt agagatcgtc agagatgcgt
    33361 tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt tgcgctttct
    33421 acagtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg cgttgccgat
    33481 gggccagaaa aaccgccctg ctctgctgcc atcaccggtg cgctgcacag ggccattact
    33541 gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg tttccgagca
    33601 tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta gtgctgtaca
    33661 gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga tgaaaaaatg
    33721 cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac gggccttagt
    33781 aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga ggcgctttat
    33841 agcgcacctt atgatgcggt gatcctggat ttaaccttac caggcatgga tggtcgcgat
    33901 attttgcgcg aatggcgaga aaaaggtcag cgtgagccgg tactgatcct gaccgcgcgc
    33961 gatgcgctgg cggaacgtgt agaagggctg cgtctgggag ctgacgatta tctgtgtaaa
    34021 ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg aaccaacggc
    34081 caagccagca acgagctgcg ccacggtaac gtcatgctcg accccggcaa acgtatcgcc
    34141 acgctggctg gcgaaccctt aacactgaaa ccaaaagaat ttgccctgct ggaattactg
    34201 atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact gtatacctgg
    34261 gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc
    34321 ggtagtgatt ttattcgtac cgtgcatggt attggttaca cattaggtga gaaatgaaat
    34381 ttacccaacg tcttagtctg cgcgtcaggc tgacgctaat ctttttaatt ctggcctcgg
    34441 tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac gtcgatgaat
    34501 tgttcgacac ccaactgatg ctgtttgcca agcggttaag tacgctcgat ctcaacgaaa
    34561 tcaacgcggc ggatcgcatg gcacagacgc caaatagatt aaaacacggt catgttgatg
    34621 acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt aatgatggcg
    34681 ataacggaga agatattccc tatagctatc aacgggaagg ttttgctgac gggcaactgg
    34741 tcggtgaaga cgatccttgg cgttttgtct ggatgacctc acctgatggc aaatatcgca
    34801 tcgttgttgg ccaggaatgg gaataccgtg aagacatggc gctggcgatt gttgccgggc
    34861 aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg gtactactgg
    34921 gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt gaccctgact
    34981 cggaaaaacc actaaacgcg actggcgtac ccagcgaagt gcggccactg gttgagtcgc
    35041 taaatcaact gttcgcccgc acacatgcga tgatggttcg tgaacgacgc tttacctccg
    35101 acgcagctca cgaacttcgt agcccgttaa cggcgctgaa agtgcaaacc gaagttgcgc
    35161 agctctctga cgatgatccg caggcgcgga aaaaagcact gctccaatta cattccggga
    35221 tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg gactcactgg
    35281 ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctgcaatcg tcggtgatgg
    35341 atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc aatgcccaca
    35401 gcatcaaacg caccgggcaa ccgctattgc taagtttgtt ggtgcgaaat ttgctggata
    35461 acgccgtgcg ctacagtcca cagggcagcg tggtagacgt cacgctgaat gctgataatt
    35521 tcatcgtgag ggataacggc cccggtgtga caccagaggc actggcgcga attggcgaac
    35581 gcttctatcg cccacccgga caaaccgcta ccggcagcgg gcttgggcta tcgattgtcc
    35641 agcgaatcgc caaattgcat ggcatgaatg ttgaatttgg gaatgcggaa caaggtggat
    35701 ttgaggcgaa ggtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat
    35761 agattttact aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaaaccaga
    35821 caaataccga gcgagaatac atatacccaa aactcgaaca tttcccgcat aaagagtttt
    35881 cttaagataa gaataataag tggcgtaaga agaaaaaatg ctgcaatcat tgcttgcaat
    35941 atacgatctt cgcctgaaac ggaaggtata ctgcaaatat ttcctcccga ctcaagcatc
    36001 cagtcatact tattgaaagc aaaaaaaaga atctattaac caataagtaa gcattatata
    36061 tagcaatgct tcaaatattg ttttaatttt ctgctttaac atatatcagt acgctcataa
    36121 aaatccattt ataattatca acatgacacg ttgatacttt catcttttac ttattcaaag
    36181 ccatattttc aatccccaaa aaaaccgaac caaacgtgta aattattccg gtgcgagtaa
    36241 gaattatgag gaatggctat cagtattgtc attttcagaa aatatttatc ctgcatcggt
    36301 gagtcagagt aagatcagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat
    36361 ttcaccgtac cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa
    36421 atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg
    36481 aacgacaccc tgaccgaagt cgcggatggc acactgcgcg accttgggca tgatgtccgc
    36541 atcgttcgcg ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct
    36601 gatgtggtga tctggcagat gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa
    36661 tatattgatg atgtattcac cgaagatcat ggaacgctgt atgccagcga tggccgtacc
    36721 cgcaaagatc cgtcaaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg
    36781 ctttctctga cctggaacgc accaatggaa gccttcaccg aaaaagatca attcttccac
    36841 ggcgttggcg tcgacggtgt gtatctgccg ttccataaag caaaccaatt ccttggcatg
    36901 gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat
    36961 actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct caggctttag
    37021 aaggagttaa ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac
    37081 cgtcaggcgg tattggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt
    37141 tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca
    37201 ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcaccttga agcgcatctg
    37261 caaaccccgc acatgaaggc gtacagcgaa gccgtaaaag gcgacgtgct ggagatgaat
    37321 atccgcatcc tgcaacctgg gatttaaacc tgccttgttt gcccggccat cctgaccggg
    37381 caatgttctt tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg caaccagttg
    37441 cggcgatctt ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc agtacgctga
    37501 tcgtcttcat catcgatagt caactgcacc agacggcgag tgttcggatc aagcgtggtt
    37561 tcgcgcaatt gcatcgggtt catttccccg agacctttaa aacgctggac gttcggcttg
    37621 cctttcttgc gttttaattg ctcaagtacg ccttctttct cttcttccgt cagcgcgtaa
    37681 taaacctctt tcccgagatc aatacggtag agcggtggca gtgcgacgta aacgtgaccg
    37741 tgtttcacca acgcgcggaa gtgttttacg aacaaagcgc agagcagcgt ggcaatgtgc
    37801 agaccatcag agtccgcatc cgcgaggata cagattttgc cataacgaag ctggctcaga
    37861 tcgtcgctgt caggatcgat accgatcgct accgaaatat catgcacttc ctgcgcggcc
    37921 agcacttcgt cggaagaaac ttcccaggtg ttaaggatct tacctttcag tggcatgatc
    37981 gcctgatatt cgcgatcgcg cgcctgcttg gcagatccgc ctgcagagtc accttccaca
    38041 aggaacagtt cggtacggtt aagatcctgc gcggtacagt cagccagttt gccaggcagc
    38101 gccgggccgc tggtcagctt tttacgcacc actttcttag ccgcacgcat acgacgctgg
    38161 gcgctggaaa tcgccatctc tgccagcaac tccgccgcct gaacgttctg gttcagccac
    38221 aggataaagg catctttcac cacgccagaa acgaatgccg cgcactgacg cgaagagaga
    38281 cgctctttgg tctgcccggc aaactgcgga tcctgcattt ttactgacag cacataggcg
    38341 cagcgatccc agatatcttc cgccgacaac tttacaccgc gcggcagaat gttgcggtat
    38401 tcacagaact cacgcatcgc gtccaacagg ccctgacgca gaccgttaac gtgcgtaccg
    38461 ccctgcatcg ttgggataag gttgacgtag ctttcggtca gcagttcacc gccttccggc
    38521 agccacagta gcgcccagtc cacagcttca gtatcaccag cgaaattacc gataaacggt
    38581 ttttctggca gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt cagaccgtcc
    38641 tgatagcacc agcgctgttc ggtgttgttg atctcatctt taaaggtaat ctcaacgccg
    38701 gggcacagta ccgctttggc tttcagcaca tgcgtcaggc gtgaaacaga aaatcgcggg
    38761 ctgtcaaaga aggtttcatc cggccagaag tgcacgctgg taccggtatt gcgtttaccg
    38821 caagtgccga caacctgtaa atcctgcacc ttttcgccat tttcaaaggc gatgttataa
    38881 acctgaccat cgcggcgcac gttaacttca acgcgcttcg acagggcgtt aaccaccgaa
    38941 atccccacgc catgcaggcc gccagagaac tggtaatttt tgttagagaa tttaccgccc
    39001 gcgtgcaaac ggcaaagaat cagttcaacc gccggtacac cctcttccgg gtgaatatcc
    39061 accggcatcc cgcgcccatc gtcaatcact tctaacgact ggtcagcatg taagattacg
    39121 tccacgcgtt ttgcgtgacc cgccagtgct tcatcgacac tgttatcaat aacttcttgc
    39181 cccaaatggt tagggcgagt ggtatcggta tacatccccg gacggcggcg aaccggctca
    39241 agcccggtga gtacctcaat ggcatcagcg ttataagttt gcgtcatggt ttaagttagc
    39301 aattcgagtt gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg ggttgaaata
    39361 atcttcgaag cccgtgaatg cgtggttgcc gccttctatg acagtctggc ggcaggaagc
    39421 gtagtacgcc accgcctggc ggtaatccag cacttcatct cccgtctgtt gcagcagcca
    39481 gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact ttgagatcgt aaatatggcg
    39541 tgactctagc acatattgct gcccggtgta ggggttctcg ttctgaccga gatagtccgt
    39601 cagcagttca aacgggcgta ccgccgggtt taccaccact gcgggcagca taaaacattg
    39661 cgacaaccag gtggcgtaat atccccccag tgacgaaccg acaataccca gcgaatcgcc
    39721 gccatgttcc aggacaatgg attccagcag ttctgccgcg tcggaaggat acggcggcaa
    39781 ctgcggaatg atcatctcaa cgtcagggtg atgttccgcc agccagtttt ttaacaagct
    39841 cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg tagacatcag
    39901 tagccttctg aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg
    39961 gtcagcgtgc catcagcatg taactcgaga gtacgccagc cgggcgcgat ggtgtccagc
    40021 gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg acggcgttgc cagcaggcgg
    40081 cgaccgttcc agtcgagatc cagctcctga tgaatatgac cgcacagcaa gtatttgacg
    40141 tgcggaaact tcgccagcac ggtatccagt tcgcccgcgt tacgcaggct gtgttgatcg
    40201 agccaactac aacccgcagg tagcggatga tgatgcagca gcagcaacgt atggcgtcct
    40261 ggcgcatcgg ccagtttacg ttccagccac tcaagctgaa actcgctcag ctcaccgtgc
    40321 ggcacgccaa acacctggct atccagcaac aggatttgcc attgctcacc aataaacacg
    40381 cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg
    40441 tggttgcccg gcagccagac gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc
    40501 tgataggccg cagaggattg atcctgcgct aaatcacctg tcgcgacaat caggtcgaat
    40561 tcgtgctggt gtggacgaat cgcctccagc accgcctggt agctttccca ggtgtttacc
    40621 cctaacaggg cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg taaaatcctg
    40681 actctggcct caccagccag aggaagggtt aacaggcttt ccaaatggtg tccttaggtt
    40741 tcacgacgct agtaaaccgg aatcgccatc gctccatgtg ctaaacagta tcgcaaccag
    40801 tccgctaaaa actggttaat ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga
    40861 taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc agccaccatc
    40921 gcgtcatgat acagacgcac cgtcattgac ggaaggctcc agtaactgat cgcgggcgca
    40981 gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat
    41041 tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa
    41101 cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg
    41161 taacgcttca tttatgccca ctcatttttt aacgcttgat gatgcagctg cagccattgc
    41221 aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt ctacccactg gtatgcctgt
    41281 tcccggctta ccacatgaac gcgaatatct tcgttttcat cagccagacc atgaataccg
    41341 cttgcggtcg tggcgtccac ttcgcccacc ataattgacg aacgctcact ggtgcccccc
    41401 gggcttgcca ggaaacttaa caccagtttg gtccgtttga ctatcagtcc cgcctcttca
    41461 atcgcttcgc gacgggcaac atcttccaca ctttcaccct cttcaatcat cccggcaacc
    41521 atctccagta gccagggggt ttcactggtg tcgtatgcgg caatccgaat ctgctcaatc
    41581 agcacaactt catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca
    41641 aaaatttccc gccgcacctc atgactcatt tgcccgttga atagacgatg acgaaatcta
    41701 taaagatcta atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat ttctacatcg
    41761 tttttgccaa atgtaacggg caggttgtct ggcttaagca ttgttaatgt cctggcacta
    41821 atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag tcatgtgtta
    41881 aattgaggca cattaacgcc ctatggcacg taacgccaac cttttgcggt agcggcttct
    41941 gctagaatcc gcaataattt tacagtttga tcgcgctaaa tactgcttca ccacaaggaa
    42001 tgcaaatgaa gaaattgctc cccattctta tcggcctgag cctttctggg ttcagttcgt
    42061 tgagccaggc cgagaacttg atgcaagttt atcagcaagc acgccttagt aacccggaat
    42121 tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc
    42181 cattactgcc acagctaggt ttaggtgcag attacaccta tagcaacggc taccgcgacg
    42241 cgaacggcat caactctaac gcgaccagtg cgtccctgca gttaactcaa tccatttttg
    42301 atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt caggacgtca
    42361 cgtatcagac cgatcagcaa accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt
    42421 tgaatgctat tgacgttctt tcctatacac aggcacaaaa agaagcgatc taccgtcaat
    42481 tagatcaaac cacccaacgt tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg
    42541 cccgcgcaca gtacgatacc gtgctggcga acgaagtgac cgcacgtaat aaccttgata
    42601 acgcggtaga gcagctgcgc cagatcaccg gtaactacta tccggaactg gctgcgctga
    42661 atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa cgcgctgctg aaagaagccg
    42721 aaaaacgcaa cctgtcgctg ttacaggcac gcttgagcca ggacctggcg cgcgagcaaa
    42781 ttcgccaggc gcaggatggt cacttaccga ctctggattt aacggcttct accgggattt
    42841 ctgacacctc ttatagcggt tcgaaaaccc gtggtgccgc tggtacccag tatgacgata
    42901 gcaatatggg ccagaacaaa gttggcctga
//
LOCUS       DACAPB010000012        41924 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.012, whole genome shotgun sequence.
ACCESSION   DACAPB010000012 DACAPB010000000
VERSION     DACAPB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.012"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..434)
                     /gene="ynaI"
                     /locus_tag="IT904_000599"
     CDS             complement(<1..434)
                     /gene="ynaI"
                     /locus_tag="IT904_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAP3190431.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSG"
     gene            678..935
                     /locus_tag="IT904_000600"
     CDS             678..935
                     /locus_tag="IT904_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HAP3190432.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(985..1935)
                     /gene="uspE"
                     /locus_tag="IT904_000601"
     CDS             complement(985..1935)
                     /gene="uspE"
                     /locus_tag="IT904_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAP3190433.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(2087..2839)
                     /locus_tag="IT904_000602"
     CDS             complement(2087..2839)
                     /locus_tag="IT904_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAP3190434.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(3034..3549)
                     /gene="ogt"
                     /locus_tag="IT904_000603"
     CDS             complement(3034..3549)
                     /gene="ogt"
                     /locus_tag="IT904_000603"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP3190435.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFTGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3560..5086)
                     /gene="abgT"
                     /locus_tag="IT904_000604"
     CDS             complement(3560..5086)
                     /gene="abgT"
                     /locus_tag="IT904_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HAP3190436.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPQNGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGISPRLS
                     "
     gene            complement(5123..6568)
                     /locus_tag="IT904_000605"
     CDS             complement(5123..6568)
                     /locus_tag="IT904_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit B"
                     /protein_id="HAP3190437.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(6568..7878)
                     /locus_tag="IT904_000606"
     CDS             complement(6568..7878)
                     /locus_tag="IT904_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAP3190438.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            8054..8416
                     /locus_tag="IT904_000607"
     CDS             8054..8416
                     /locus_tag="IT904_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3190439.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAALSLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQRRDSRQTP
                     LTASLITQFRRECGYLQS"
     gene            8746..9309
                     /gene="smrA"
                     /locus_tag="IT904_000608"
     CDS             8746..9309
                     /gene="smrA"
                     /locus_tag="IT904_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAP3190440.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(9330..10562)
                     /gene="dgcM"
                     /locus_tag="IT904_000609"
     CDS             complement(9330..10562)
                     /gene="dgcM"
                     /locus_tag="IT904_000609"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAP3190441.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            10817..11800
                     /gene="zntB"
                     /locus_tag="IT904_000610"
     CDS             10817..11800
                     /gene="zntB"
                     /locus_tag="IT904_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAP3190442.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            12278..13651
                     /gene="dbpA"
                     /locus_tag="IT904_000611"
     CDS             12278..13651
                     /gene="dbpA"
                     /locus_tag="IT904_000611"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAP3190443.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIATLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(13780..14715)
                     /gene="ttcA"
                     /locus_tag="IT904_000612"
     CDS             complement(13780..14715)
                     /gene="ttcA"
                     /locus_tag="IT904_000612"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAP3190444.1"
                     /translation="MQENQKNTKKEIYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14767..16002)
                     /locus_tag="IT904_000613"
     CDS             complement(14767..16002)
                     /locus_tag="IT904_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP3190445.1"
                     /translation="MSKLPTGVEIRGRNIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISYIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRMCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            complement(16004..16219)
                     /gene="xisR"
                     /locus_tag="IT904_000614"
     CDS             complement(16004..16219)
                     /gene="xisR"
                     /locus_tag="IT904_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAP3190446.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            complement(16319..16507)
                     /locus_tag="IT904_000615"
     CDS             complement(16319..16507)
                     /locus_tag="IT904_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAP3190447.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(16500..16694)
                     /gene="ralR"
                     /locus_tag="IT904_000616"
     CDS             complement(16500..16694)
                     /gene="ralR"
                     /locus_tag="IT904_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HAP3190448.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKDISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTENINGGVHV"
     gene            complement(16751..17584)
                     /gene="recT"
                     /locus_tag="IT904_000617"
     CDS             complement(16751..17584)
                     /gene="recT"
                     /locus_tag="IT904_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HAP3190449.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVNDKDVLCVINSPAMKAQLAA
                     ALPRHMTAERMIRIATTEIRKVPELRNCDSTSFIGAIVQCSQLGLEPGSALGHAYLLP
                     FGNGKAKNGKKNVQLIIGYRGMIDLARRSGQIISLSARVVRECDEFSYELGLDEKLVH
                     RPGENEDAPITHVYAVAKLKDGGVQFEVMTRKQVEKVRDTHSKAAKNAASKGASSIWD
                     EHFEDMAKKTVIRKLFKYLPVSIEIQRAVSMDGKEVETINPDDISVIAGEYSVIDNPE
                     E"
     gene            complement(17577..20177)
                     /gene="recE"
                     /locus_tag="IT904_000618"
     CDS             complement(17577..20177)
                     /gene="recE"
                     /locus_tag="IT904_000618"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAP3190450.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     DPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQNQPETEIISPDAKQPEPV
                     VQQEQEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQVEAKEKDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTSYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            complement(20279..20554)
                     /locus_tag="IT904_000619"
     CDS             complement(20279..20554)
                     /locus_tag="IT904_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HAP3190451.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            complement(20629..20799)
                     /locus_tag="IT904_000620"
     CDS             complement(20629..20799)
                     /locus_tag="IT904_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190452.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGVLSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            complement(20799..21020)
                     /gene="kilR"
                     /locus_tag="IT904_000621"
     CDS             complement(20799..21020)
                     /gene="kilR"
                     /locus_tag="IT904_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HAP3190453.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            21523..21951
                     /locus_tag="IT904_000622"
     CDS             21523..21951
                     /locus_tag="IT904_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001634365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein B"
                     /protein_id="HAP3190454.1"
                     /translation="MYTIIIFGLTLWLIPKEFAVVFNAYTEITWLFQIIVFAFSFVVA
                     ISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFLKTGNLIITSPCRNPVMKKLE
                     RKGIIQHQSDGANCSYYLVTEKYSHFMKLFWNSRSRRFNR"
     gene            complement(21948..22103)
                     /locus_tag="IT904_000623"
     CDS             complement(21948..22103)
                     /locus_tag="IT904_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAP3190455.1"
                     /translation="MDTIDLSNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEVGARRW
                     LEKYTVS"
     gene            complement(22235..22513)
                     /locus_tag="IT904_000624"
     CDS             complement(22235..22513)
                     /locus_tag="IT904_000624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190456.1"
                     /translation="MIADEKQGLVLKIDTSPLRHQIEKLYKLLVPAVFNCPDDFIDCF
                     IKVFTEIRIFDFRKTITADKTIIVRARVLIDLNVARSAMRTAKFNIHN"
     gene            complement(22701..23108)
                     /locus_tag="IT904_000625"
     CDS             complement(22701..23108)
                     /locus_tag="IT904_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3190457.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQSGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDAPVSLNSPLDLSEDELEMLRLYRALPKS
                     EQQAQISELSARVENFNRLFTELLEARKRNKHQ"
     gene            23186..23413
                     /locus_tag="IT904_000626"
     CDS             23186..23413
                     /locus_tag="IT904_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP3190458.1"
                     /translation="MFKSDVINYYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQE
                     ASGGELQYDSNIYDKYRKAKRAGKGIHENQA"
     gene            23397..23819
                     /locus_tag="IT904_000627"
     CDS             23397..23819
                     /locus_tag="IT904_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190459.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGESELALAP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAILAILPRELRHRLSVFDTLERRALLA
                     AQEALNTAIDAHDDAVQAVYRKAHFSRGGSSGDSVVVH"
     gene            23898..24692
                     /locus_tag="IT904_000628"
     CDS             23898..24692
                     /locus_tag="IT904_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190460.1"
                     /translation="MASNWIKLEVITPDKPEIFRIAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVESDGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPEEEEEEEEDKNTDLPLNPPRQKR
                     ASKKFEPEAIALPDWLPETLWNEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFTP
                     EQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            24699..25484
                     /locus_tag="IT904_000629"
     CDS             24699..25484
                     /locus_tag="IT904_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP3190461.1"
                     /translation="MKNIATGGVLERIRRLAPSHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNDGQRYALSQAKSIADELMVGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKNGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQCETKNEQVVLHQIVDRRTASIRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIEKQFLPGGNFNGDRF"
     gene            25468..26193
                     /locus_tag="IT904_000630"
     CDS             25468..26193
                     /locus_tag="IT904_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAP3190462.1"
                     /translation="METVFDALKAMGKASSQEVAARLGMTRDEAINELWKLKRRGEAD
                     NKGPMWWLTQTDENASVAQASKVTAQMLIEAIEQHGPKAADELALIFRITSRRVNSSL
                     AMAISKGRLIRVNQNGKFHYCLPDGNLPAEPKVVSVVKTPGKGFPQPAGVALPVQEAA
                     TQEEIKTDTVGDIVQSLPSFTETRANDLILPSLQMANRELRRAKSHVQKWERVCAALR
                     ELNKHQDIVRQIVDSSSRIVSEK"
     gene            26210..26626
                     /locus_tag="IT904_000631"
     CDS             26210..26626
                     /locus_tag="IT904_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP3190463.1"
                     /translation="MAKVLTQEEREKIKGQIVDLVRQSGRETLRQLEAKTGATRYLIS
                     ILAKELVSSGDVYNSGYGLFPSEQARQDWQNARKKLSRAKSKEPVMVDPDLIWSLPDG
                     EIRRYDRCQNIICRECRKSEVMQRVLAFYQGNVGIY"
     gene            26978..28513
                     /locus_tag="IT904_000632"
     CDS             26978..28513
                     /locus_tag="IT904_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190464.1"
                     /translation="MITREIMTPPTKIDTSSLLTILGVIAAVWALITPNARLRLRFCL
                     AWWDWAIVGFAFLLSNYLVFAPALKSLDLYFSFGPWKWGLDSSSAVYLISLAVAIYIL
                     FRLKKPKLSVGRTGIFLELVENLHLTKRYDDLAQLLAPQLEKLISIIDSPVKNRLCDK
                     IANNLRISNRETAAEYAHEALLNIVSSPELTNHFALAHPSLCLELIKIEPIVRSDFTS
                     NFISALLDSPNSRLYVELKNNLNVSRGHRLLIPKNNRILHFFFSNAKFAADLAIYRDI
                     GEYIYWRLDEDEKIIATLNKSLGSYSDVSKYKCPIYSGITLFEIMVHEGIHQGLQDHL
                     WLHYYTHFAKKIIKNMNRQSNEYSGEWETPFHFLLCHLFSIAINWAEQCEWIDERDIL
                     QENKETENFDLHYISKEATKLLGAMLELVLPNSKLTLKSRKDILGIIVSCYIRLKRNK
                     KLKDVADSLLIFTTRGEGNLASPYYRKELLEIFNTLDDYRLRSDAPEFREAIESAIQA
                     RPN"
     gene            complement(28610..29798)
                     /locus_tag="IT904_000633"
                     /pseudo
     CDS             complement(28610..29798)
                     /locus_tag="IT904_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            30197..32758
                     /locus_tag="IT904_000634"
     CDS             30197..32758
                     /locus_tag="IT904_000634"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190465.1"
                     /translation="MLKEYLEGSGYDIELRKKESSLEYDIEGFSRATGQKIIGEAKAH
                     SKSMSGQTVAAFVGKLLPLGLLEKKVHGLFLSLSPLTPEAEDYFRTVENMGLTTKVGK
                     ELYNQITATLHLPTETSLHKEIKGYDLVAPNILKTDTGLYKLFILKEVNSGTPSNFVV
                     FNKSGSQIDDERFLENLKIGISELAGLDFIMPSKKKIVDEMPQVKREIVRGLTVGTEW
                     ADYRLPAGPTVFVGRTEFIGELLSHIKHNELPHVLQIKSRSGVGKSSLVSFLENKLSM
                     DGVITELHDSRDVKTIYDVFYLVQRFTQSSIIATNFIELDEQLKDLQLSLNGQKAVFF
                     VDQFESTFSNPDIFDCYEYIANSITKLRGGVYIVFARKNDQLTTYDNSKVSLNRINQL
                     SKSFTLPDFENKESILLLEKINENNSNILSKEILPYIIEFAQGFPWLLKRTIAHVLKL
                     TRKGDSQRDLISAGLKLNDLFEEELEGLDEIEKDYLVKIAAKLPADFNELQYQFDEDP
                     MLVKVLDKLTAYRLVRLSGSTYDTYNDVFKEYLVYQKLPEFRLLVIYRMYPSSVISQF
                     HKLITMRKLTITKITENLNVREGYAFNLVKELRNLDLLDAVKSGEWIIPTNVRDIYSQ
                     GTLAGYLRRKILDNQLVETLLKKILSGEKVFESDIPTYFSESYPFIEASEKTWGLYST
                     ILKSWLLALKIIEIDGEGRIIASDVSHNDATAELGNLKKISGLRAGNKGLFFPVTSHA
                     KMIEVTSLILNGKEPTGKEALKAKSDLNNAGVLVDGHLAVSNIEELNEALTAQILDDG
                     YEDFWKAIHAGQPCYEIFKGIAGEGLTESTLKWRLRKLTALAKKLKIIPNKRFKY"
     gene            complement(33152..33259)
                     /locus_tag="IT904_000635"
     CDS             complement(33152..33259)
                     /locus_tag="IT904_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190466.1"
                     /translation="MLTGAFLYLPLVFMPEADSLKHPQLFYLTPVTSPI"
     gene            33362..33517
                     /gene="hokD"
                     /locus_tag="IT904_000636"
     CDS             33362..33517
                     /gene="hokD"
                     /locus_tag="IT904_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAP3190467.1"
                     /translation="MKQQKAMLIALIVICLTVVVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            33685..33963
                     /locus_tag="IT904_000637"
     CDS             33685..33963
                     /locus_tag="IT904_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190468.1"
                     /translation="MAHDTKLHNSNDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRSSRTYQQRIWR"
     gene            33965..35023
                     /locus_tag="IT904_000638"
     CDS             33965..35023
                     /locus_tag="IT904_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAP3190469.1"
                     /translation="MRILLRPVLVPELGLVVLRPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDDRVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADALPESVLCRSLGLPVEKIRSVYRESDIVPGEQTATSILKQR
                     TKNIALPLHVHQQQPPLQEKTLVSIAVDPESPVQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            35024..35404
                     /locus_tag="IT904_000639"
     CDS             35024..35404
                     /locus_tag="IT904_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP3190470.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLAIKVIAEPPNKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMHEEQVKK"
     gene            35401..36222
                     /locus_tag="IT904_000640"
     CDS             35401..36222
                     /locus_tag="IT904_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP3190471.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPLVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYSSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTETFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            36366..36441
                     /locus_tag="IT904_000641"
     tRNA            36366..36441
                     /locus_tag="IT904_000641"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36399..36401,aa:Ile,seq:cat)
     gene            36617..36703
                     /locus_tag="IT904_000642"
     CDS             36617..36703
                     /locus_tag="IT904_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAP3190472.1"
                     /translation="MVDFSSRIMSVLSDGVLVAGVWLLLSRC"
     gene            complement(37192..37404)
                     /locus_tag="IT904_000643"
     CDS             complement(37192..37404)
                     /locus_tag="IT904_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190473.1"
                     /translation="MPLHGAGCLPVNSVPAPESAIIPYTYSLITPPHRGIHHAKFFNK
                     LPAGQTIIASLNYEQRGILRENCFLQ"
     gene            complement(37475..37810)
                     /gene="iraM"
                     /locus_tag="IT904_000644"
     CDS             complement(37475..37810)
                     /gene="iraM"
                     /locus_tag="IT904_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAP3190474.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKVKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCILQIC
                     PYGLKQQSP"
     gene            38071..38259
                     /locus_tag="IT904_000645"
     CDS             38071..38259
                     /locus_tag="IT904_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAP3190475.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            38256..38417
                     /locus_tag="IT904_000646"
     CDS             38256..38417
                     /locus_tag="IT904_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190476.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINTPASS"
     gene            38567..38782
                     /locus_tag="IT904_000647"
     CDS             38567..38782
                     /locus_tag="IT904_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP3190477.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            38787..39137
                     /locus_tag="IT904_000648"
     CDS             38787..39137
                     /locus_tag="IT904_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAP3190478.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            39201..39734
                     /locus_tag="IT904_000649"
     CDS             39201..39734
                     /locus_tag="IT904_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAP3190479.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVLLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIEQ"
     gene            39951..40133
                     /locus_tag="IT904_000650"
     CDS             39951..40133
                     /locus_tag="IT904_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190480.1"
                     /translation="MRKLKMKLCALMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPAP
                     DWQTPLNGIISPSERG"
     gene            complement(40224..40517)
                     /gene="iss"
                     /locus_tag="IT904_000651"
     CDS             complement(40224..40517)
                     /gene="iss"
                     /locus_tag="IT904_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HAP3190481.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            40661..>41924
                     /locus_tag="IT904_000652"
     CDS             join(40661..41021,41021..>41924)
                     /locus_tag="IT904_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HAP3190482.1"
                     /translation="MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLL
                     GKKTMENELLKEAVEYGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDD
                     WMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRI
                     MRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCC
                     DREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQF
                     ARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNE
                     WHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT        10898 a         9454 c        10219 g        11353 t
ORIGIN      
        1 aagccagaaa ggctcatgcc gaaatgttcg ccataaagca gaacaagcac cacaataatg
       61 gtgattttga tgatacgcga cataatccgc gcactggtga tatcgcgacc ttttttaatc
      121 tgctgttttt caaactgatt aatcagcaga aatagcttaa tcgtcagaat aaccgcaatc
      181 agggacgtac agataaaatc gataacgcct ggggtgataa atttgagttt atagttttct
      241 ataacataat taataatgct accaacagca ctgataatta tggtgtagat taaaaattgc
      301 accgcatgga ataaaaatcc ttttctttta cgatttccac ggcgaaacca aaagctcatc
      361 agaatcaatg ctgcgcagct accgaaaata atgaccagat taagcgcatt atttgtaaac
      421 agttcagcga tcattgtttt atcaggctcc tccagataat tgtcgtcatg ccggaaaccc
      481 ctggcggggc tattttaccg cgacaattca ttcagatcat caatagtcag ggaaggaagt
      541 agcaacatta gctaactaat caatgcgttg attgtaaatc tagctaagag gtgaggtttt
      601 cagagcagac aacggtgaaa tgtcatggta ttgttacgtt taggtaacaa gaaatttgtc
      661 tgcacaagga ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc
      721 tctttggttt gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg
      781 gcggcgttat agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga
      841 ttcagtcatc gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt
      901 tgggaattta tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg
      961 gccttgtcag ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt
     1021 ctgatactgg tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc
     1081 cgccgtgttg ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac
     1141 aattcccgcc tgtaaatgct ccgccaaatc aggaatcacc tcttcaggca gacctttttc
     1201 tacgtgcgtc atgttttcat taatgccgaa tttctgccgc agagctttca ttgccagcaa
     1261 atgttgccca cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc
     1321 gatattaatt ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg
     1381 ttctgccagt tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc
     1441 ttcactggcg agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt
     1501 caccatccac accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat
     1561 caccgcttcc agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct
     1621 gatcacttcc tgaataatgg cttcgaaagg acggttatgc cagaccactt taatttcaat
     1681 gggaacgcca gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct
     1741 gatgacgccc tgacgcatag cggtacgttc gtccggggag agcagggtgg tcatttcgta
     1801 tgagaagtca tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata
     1861 aacagctcgc cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt
     1921 ctgatacata gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa
     1981 gattaaccca tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag
     2041 aaaaatttaa tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac
     2101 gcgtatgacc agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt
     2161 tgactgccag cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg
     2221 tcaggcccag atagttaccg atatcgccac gagtcatcgt caggcggaat tcacgagggg
     2281 agaagccgcg ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct
     2341 cggcattttt cttcgacaac agcaggatca tgtcctgatc gcctttgatt tcaccgctca
     2401 tcagacgcat catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt
     2461 cgaacgggat ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc
     2521 cgctgccgat agcatcaaat cccaccaggt cacctgctaa atgaaaacca gtgatttgct
     2581 cgtcgccttg ctcagtgatg gtataacttt taatcgtacc ggagcggatg gcataaagcg
     2641 atttaagttc atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct
     2701 caatgatatt atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct
     2761 ggctgatgct gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa
     2821 ttcgcttttc cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca
     2881 tctttttagg gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga
     2941 tgtaaatata tgtctatcta tttgaaaacc attaagttgt taagggtaac tttacataaa
     3001 agtgtgaaca agctggcaca aattgtttaa tgtttacagc aaaagataac cttcatggcg
     3061 caataaccac tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc
     3121 aataacccga tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac
     3181 ggcacgcgcc gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg
     3241 cccgcagggg atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccctgt
     3301 ggcagtggga agcgtatcaa taatgctaag attaccggta aaataatcac gaagcttgtc
     3361 gcttaaaccg cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat
     3421 gtccagcagc tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcagacgaaa
     3481 ttgttcatcg caaatcaccc acagtggacc aagtggcgtg gcaatttttt cttcaagtaa
     3541 tctcagcatc cgttctctct taagacaaac gtggggaaat ccccggacct atcggcagac
     3601 caacaagata ccacgccagc aacatcagca gccataccac caaaaagata agcggatagg
     3661 gcaagactaa cgaatagtaa gtacccagtt tcgcgtctgg tttgtagcgt tgcaggaatc
     3721 caagaaacag tggaacaaaa ggagataccg gcgctaaagg caatacggat gagtcggcaa
     3781 tacgaaagag gatttgcgca aatgccgggt gaaagccaag tagcataaac attggtacga
     3841 aaatgggggc aagaatcgac cagattgcgg aaccgctggc aataaacatg cataagaaag
     3901 aggaaagcaa cgccagaccg acaaacgccg ggatgccgct aagccctgaa ctttccagta
     3961 tatcggtcag ccccacggcg atgaatttcc ccatgttgct ccagttaaac atggcgacaa
     4021 attgggcgag gggaaaaacc atcacgataa atcccgccat ctctttcatc ggttcaatca
     4081 ttaaatgcgg taaatccgcc tgacgtcgaa ttgtgcgggt agcgatgcca taagccagcg
     4141 agacaacaaa gaaaaaaaga atgatcagtg gcacgatacc tttaataaag ggtgatggca
     4201 tcacggtgtg attaatcgga tcgcgcaata tcccgttttg cgggatcacc atcagcgcaa
     4261 tcgcagcaat aaaaagtagc gatacgacac ctgctatgcg taaaccaaaa cgctgacttt
     4321 cggtcaatgt ctgcagtttc tcatcgctgt ttccctgcca ttgacctaac cgtggctcga
     4381 tgattttgtc ggttatcagg ccgccaacaa tcgtcagtac gactacggag ctggccataa
     4441 aataccagtt atcaattaca ctgacgtgca tttgcggatt gaacgcagct gccgcttccg
     4501 tgctgatccc cgacagcaac acgtcggttg tgacaatcag taaattagcc gtaaagccgc
     4561 aacctacgcc tgcaatggca gccagtaaac ctgcaactgg atgcctgccc accgccagaa
     4621 aaatcagcgc acccatcggt ggcatgatca ctaacgccgc atcggaagaa atgtggctga
     4681 aaaaagcaat aaacagcacc atataactgg cgtagcgggc attaacatgc gatgccattt
     4741 taaccattag tgctggcagt aagccgacgc gctccgccag accggcacct aaaaccagcg
     4801 ccaggatcgc accaagtgga gcaaaaccgc taaagttttt aataacattg ggtaaaaacc
     4861 agtgtaatcc ttccacactg agcaggtttt tcaccaccac cggcgtacca tcggtcgggt
     4921 ttttcgcact gacgccaaag gccgacaaaa ttgccgtcgt caccatgagt acgataatca
     4981 aatagataaa gagcagaaaa ggatggggaa ccttgttacc aattctttcg acccagccat
     5041 agagcttccc ggattgggag gacgacggta tggatgacat actcatgggc attcctcggt
     5101 tgttgtgttt gcgttgttgt tgttatttta aaggtgacgg tgtcacgttt ttcgggatag
     5161 ggcagtgata cggttgcgtg tccgttactt gctgatgctc ttgttggcat tcttgcaata
     5221 gccctgaatc aaggaagaga ttgactgtgg ttgctgccat agttttcgcc gccagcagca
     5281 ttcctttatg agcaatagat gttcgcccct ggctaaccaa ttgccacgta tgtagcggtg
     5341 taccgacggc aaaacagggg ctgaaacact gggcaacagg cagtttccaa ctgacgtcgc
     5401 cgacatcagt cgatgccgca agcacgttat cggtggcggc atatggagcg acttcattcg
     5461 ccagtaccgt ttcacgatga cgtagtgcaa aaaccttgcc gttttcgcca ccggttgcgg
     5521 cgatattatt cagactgttt tgccgatcgt tggaggtgag cgtagcctga atttgtttcg
     5581 caaaagccag ttcttcggag ttccattccg gggtaccaaa atgggatagg gcctggtaca
     5641 tggcattttc taaggtgcga ttcgggagat aactggaaca ggctttgtcg aagcggcatt
     5701 caaccgtggt ttcggtcatc aatgccgcac cttcggcgat tttggcgacc cgatcataaa
     5761 tatgctgcac gtcggtcatt tcgggggcgc ggataagata aagcacttct gcctgcgcct
     5821 ggaccacgtt gggcgagatc ccgccgctat ttgtgatggc atagtgtacg cgcgcttttt
     5881 caataatatg ttcgttgagg aagttggtgc cagtggtcat caacgttacg gcatcaaggg
     5941 cgctgcgtcc caaatgaggg gaattcgcgg catgtgctgc gatcccttta aagcgccatg
     6001 atgcctgaat gtttgccagc gtgcgggtat tgaacatacc ggcaaaggct tccgggtgcc
     6061 aggtgagtgc cgcatccaca tcatcaaata ccccctcgcg aaccatgaac gttttacccg
     6121 agccgccttc ttcgccagga caaccataaa agcgcaccgt gccgccttgc ccatattgtt
     6181 ccagccattt cttgacggct attgcagcgg caaaggcggc ggttcccagc aaattgtgtc
     6241 cgcaaccgtg accattttca ccgggcgtca cggatgtagg ttgcgcgcaa cctgcttgct
     6301 gacttaaacc tgccagggcg tcatattctc ccagcagggc gataaccggt ttgccttgac
     6361 caaacgaagc aataaaggca tttgggatat tgcctacgtt gcgggtaacg gtgaagcctg
     6421 cagattccag cgccgaagcc agatgctccg ctgaccagaa ctcttcaaaa cgtgtttctg
     6481 gatgatccca gatttgatcg gcaatatcgg tataacgttg gcgatcggct tcaatcgcat
     6541 cgtcgataaa acgatagatt tcctgcatca gatacctcgc gtccagggaa aattgagcgc
     6601 ggtgcgcgcc agcgtttcga cggcaatagc gagaacctgc tcgtcaaaat cgaatttttc
     6661 gttgtgatga cctgccgcca gctgtgtgcc aaacaccacg taggaggctt gcccttgatg
     6721 ttgctgcacg cgggccatca ttaatgtggc atcttcggaa cccgcaggcg cttcaacacg
     6781 ttcaatggcc tgattgaccc ccgcgacctg agccgcctga ctttgcaacc atgcgaccca
     6841 ttgcggcgaa ggagaactgg cggtagctgc acccatcaga cgagtttcaa cgccgacacc
     6901 atacatggtt gctgcgccct gaatcgcttg ttgtgcacgg tcaaaaacat attgattaat
     6961 gacgtcgctg gccccgcgtg tttctacttt cagcaacgcc gaggcaggaa caacgttacg
     7021 accgcttcct gcctgcataa cgcccacgtt tactctggaa gctccttcgc tgtgcggggc
     7081 gattgcatgc agtgcaagag tggcttgtgc tgccgccaac aaggcattgt gaccgtcttc
     7141 tggttttgcg cctgcgtgag cggcggtacc ggtgaagtgc gcgtcaaatt tggtggttgc
     7201 cataaaatta tcactgccgc acaccacggt gcccgcaggt acgccagtgc caatgtgcac
     7261 ggcagtaaaa taatcaacat catctacgac acctgcatcg accatcgccc gcgcgccacg
     7321 cgtaccttcc tctgcaggct gaaaaatcag tttgatgacg ccatgtagtc cggactcgaa
     7381 ttgtttaagg gtatgcgcca gcccaagccc aatggcggta tgtccatcat gaccacaggc
     7441 atgcatcatt ccggcgttac atgacgcaaa accgtcgcgg taggggcgat ggctgacatc
     7501 ctgctcttca ctgagatcca gcgcgtccat atcgacacgg aaagccatca ccggaccggg
     7561 gcgaccggta tccagggtgg cgacgatacc cgtgaaacca ccttcaaaaa ccgcaatcca
     7621 ttgtgctagc gcaccctgtt gacgagcgcg ctcgaattcg cgttgtagag tgagttcatc
     7681 aggtagtccc atccggctac tttcattaac tacttcgcgg cccagcgcca gtgaatagcc
     7741 gagctgctgc aattcttccg caacaagggt ggcagtgcgg aattccaccc agccagactc
     7801 tgcatagtga tgaaaatcac gtcgccagtg cgataatttt ggggcaagcg aattaacaaa
     7861 ttgattcaaa gactccataa cctttcccgt catcagtaaa aagtgtgacc cggttcacgt
     7921 agcgatagtt tttacttatc actaactgat ttttcacagt tttaaccgtt cataaattac
     7981 cctgacacaa tcatctgcat taaagtagat gccagtttct ttggtctgat aaataacggt
     8041 tatcggtggc gtcatggctt ttcaggtaaa aattcatcaa attcgggctt ttgttgaagt
     8101 ggctcgtcag ggcagcattc gcggagcgag ccgaatgttg aatatgtcgc aaccggcact
     8161 gagtaaatct attcaggagc tggaagaagg gttagcggcg ctcagtctgg tggcaaaaag
     8221 cgattttctc agcatactgc ctgaagaaat gggctgcgat cccttgcacg gacaggggct
     8281 ggtgatgttg ccggttagcg aaattttacc gaaagcggcc tattatttga ttcagcggcg
     8341 tgatagtcgc cagacaccac tgaccgcgtc attaatcacg caattccggc gagaatgcgg
     8401 ctatctgcaa agttaaaact gcataaaaaa atagagtctg tcgacatccg acagactcta
     8461 cagtacacac agcagtgcat ccgcgtctta aatccggcat tgtctcctct gcgccggtga
     8521 ctgtgttata acggttttaa cctttagttg ccaattttct tcagtgacag atttcacgaa
     8581 aatataagtt acattattaa tatcgtgaat gaataatcat gcataagtat tttgcttaaa
     8641 atatcggcaa tatttggaac ttattactgg aaatttgggt aatacgttgt tggaccgacc
     8701 cggtctggtt atcatatcgc gctcttaatt gcgggaggat gtaacatgaa ccttgacgac
     8761 aaatcgctgt ttcttgacgc catggaagat gtccagccgc tgaaacgtgc taccgatgtc
     8821 cactggcatc caacgcgtaa ccaacgtgcg ccgcagcgta tcgacacgct gcagcttgat
     8881 aatttcctca ccaccggatt tctcgacatc atcccactaa gtcagccgct ggagtttcgg
     8941 cgggaagggt tgcaacatgg ggtgctggat aagctgcgca gtggtaaata tccgcaacag
     9001 gcgagcctga atcttttgcg ccagccggtg gaagagtgcc gcaaaatggt gttcagtttt
     9061 attcaacaag ccctggcgga tggtttgcgt aacgtgctga ttattcatgg taaagggcgg
     9121 gatgataaat cgcatgccaa tattgtccgc agctatgtgg cgcgctggct gaccgaattt
     9181 gatgatgttc aggcatattg caccgcgcta ccgcatcatg gcggcagcgg ggcgtgttac
     9241 gtcgcactac gtaaaacggc gcaggcgaag caagaaaact gggagcgcca cgctaagcgc
     9301 agtcgttgat ctcgagacgc atccgcggct tatgccgcca gcacgcggtt gcgtccatca
     9361 tttttcgccc gatacaaagc atcatcaacg cgtttaaaca gttcatcgat gctttcattt
     9421 ccttcgtgat gcgccacacc aatgctgacg gtaaagcgtg gtaagcccga aatactcact
     9481 tttgccacgc ttacgcggat agtttcagcc agcgaaagcg cggtatccag tggggttctt
     9541 ggtagcaata agacaaactc ttcgcctccc caacgaaaca ccaaatcgcc tttgcgagcg
     9601 caactttcga gggtgcgggc gagggcgcat aacacctcat cacctttaga atgcccatag
     9661 agatcgttaa tgtgtttaaa acgatcggtg tcgatgagca acaagctgta atcctgagcg
     9721 atggcgagat gctgcatttg gcctggttcc gtaatgtgat aaaactgtcg ccgattcagt
     9781 aatccggtca tcgcgtcatg gtgagcagca tgttccagct gctcctccag ccgtttttgc
     9841 tcagtaatat catgcacaat acataacatg agcttgtcgc cataaatttc aatcggtccg
     9901 gcataggtct gcacatgacg agtcgaacca tccgccagtt tatgaacaaa attcaaaggt
     9961 ttatgaccac cgggtaaatg cgagatttca tgcatgatag gcatgacgcg acgcccgagc
    10021 atatttattt cccaggtatg tttctggcac atcgtttcat ggttataacc atagaaattg
    10081 agcgcggcga ggttagcatc gacgatttgt ccatctcgtg acgggtcaat caacaacatt
    10141 ggtgcagagt tagtcagaaa aaagcgcgca taaaaacctt gttttttgcg ctgataattt
    10201 gccgagcgac tggcttttaa acccagcgtt gccggcgctt cgataccttc gaaaataatc
    10261 accggttctg tttctgtcag ctttcgcaaa acaagccgac agctcaatgc tgtttcctct
    10321 tctttacgct gaacagtgag gatttcgata atatcgtgtt ggttttgcag atcggagagg
    10381 tatttcggca gttctttttg tgaggagacg gaatagggtc cggttcgtag ctgactaaac
    10441 gtgaggtctt gcatcaacag tttcgccgcg ctattggcat aaattaactg ttcctcaaag
    10501 ggcgaaacga tccagacagg actggtgagt aagtccaggg tattgaagtt gtgcgtaatc
    10561 attgagatcc cgttattttt atcaattttt gttgctatcc gatcgcaaaa aagccacgtc
    10621 atatgatcag ataattctga taatgataga cgctatttaa cacttcacac ggtttgtata
    10681 cggaaaagca ttttgctttt tgtattcaat ttagacagaa ttttattaat catttcaggg
    10741 taatggggtg atgagatgtt gcgtaacagg gccagaaggc tagactacaa aataatgcgt
    10801 tgatgatgga ggcactgtgg aagcgattaa gggatcggac gttaatgtcc cggatgcagt
    10861 atttgcctgg atgctggatg gtagaggcgg cgttaaaccg ctggaaaata cagatgtgat
    10921 tgatgaagcg catccctgtt ggctccacct taattatgta caccatgata gcgcccaatg
    10981 gctggcgaca acaccgctgc ttcccaataa cgtacgtgat gcgctggcgg gcgagagcac
    11041 gcgtccccga gtcagccgtc tcggtgaagg cacgctgatt acattgcgct gtataaacgg
    11101 cagcaccgat gaacgccccg atcaactggt cgccatgcgt gtatatatgg acgggcggtt
    11161 aattgtttcg acccgacaac gcaaagtgct ggcgctggac gatgtggtga gcgatctgga
    11221 agagggcacg ggtccgaccg attgcggggg atggctggtg gatgtgtgcg atgcgttgac
    11281 cgatcattcc agtgaattta tcgagcagct gcacgataaa attatcgacc ttgaagataa
    11341 tctccttgat cagcaaattc caccgcgtgg attcctggct ctgctgcgca aacaattaat
    11401 tgtgatgcgt cgctatatgg caccgcaacg tgatgtttat gctcgtcttg ccagtgaacg
    11461 tttgccgtgg atgagcgatg accaacgccg tcggatgcag gatattgccg atcgccttgg
    11521 gcgcggcctt gacgaaatcg acgcctgtat agcacggact ggcgtgatgg cggatgaaat
    11581 cgctcaggtg atgcaggaaa atttagctcg tcgtacctat acaatgtcgt tgatggcaat
    11641 ggtcttttta cccagtacct ttctgaccgg gttatttggc gtcaaccttg gtgggatccc
    11701 tggcggcggg tggcaattcg gattttcaat tttttgtatt ctgttagttg ttcttattgg
    11761 tggtgttgct ttatggttgc atcgtagtaa atggttgtaa caaaagcaat ttttccggct
    11821 gtctgtatac aaaaacgccg caaagtttga gcgaagtcaa taaactctct acccattcag
    11881 ggcaatatct ctcttgcagg tgaatgcaac gtcaagcgat gggcgttgcg ctccatattg
    11941 tcttacttcc ttttttgaat tactgcatag cacaattgat tcgtacgacg ccgactttga
    12001 tgagtcggct tttttttgcc tgttatttat cagcgtctac cctttaagag tccacccaat
    12061 gaccagaggg aaatatgacg acacttattt atttgcaaat tcctgtccct gaaccgattc
    12121 ctggcgatcc tgttccagtg cccgatccga tccctcgccc gcaacccatg cctgacccac
    12181 cacccgatga agaaccgatt aaattgtcgc atcgtgagcg tagatctgcg aggatacgcg
    12241 cctgctaact ttgcgtcgat gaccacgaga atagattgtg accgcttttt ctaccctgaa
    12301 tgttttgcct cccgcccaac tcacgaacct taatgagttg ggttatttaa ccatgacgcc
    12361 ggtgcaggcc gccgcgcttc cggcgatcct tgccggaaaa gatgttcgcg tgcaggcgaa
    12421 aaccggcagc ggcaaaacgg cggcttttgg cctcggcttg ttacagcaaa ttgatgcgtc
    12481 gctatttcaa acccaggctt tagtgctgtg tcctacgcgt gaactggcgg atcaggtggc
    12541 aggtgaattg cgtcggctgg cgcgttttct gccaaatacc aaaattttga cgttgtgcgg
    12601 tggtcaaccg ttcggtatgc agcgtgattc gttgcaacat gcgccgcata ttatcgtggc
    12661 aacgccgggg cgtttgctgg atcacctgca aaaaggcacg gtatcactgg atgcgttgaa
    12721 tacgctggtg atggatgagg ccgaccgcat gctggatatg ggatttagcg atgccattga
    12781 tgatgtcatc cgttttgcgc ctgcatctcg acagacgctt ctgttttcgg caacctggcc
    12841 ggaagccatc gctgcaatca gcggacgagt gcaacgcgat cctttggcga ttgaaattga
    12901 ctcaacagat gctttgccac ccattgaaca acaattttat gagacatcca gcaaaggcaa
    12961 aattcctctg ttgcaacggt tattaagctt gcatcagcca tcctcttgcg tggtgttttg
    13021 caataccaaa aaagattgcc aggctgtctg cgacgcgctg aatgaagtag ggcaaagtgc
    13081 attgtcatta cacggcgatt tggagcaacg cgatcgcgat cagaccctgg tacgttttgc
    13141 taacggtagc gcccgtgtac tggtcgcgac tgatgttgct gcgcgtggtc tggatattaa
    13201 atcgcttgag ctggtggtga actttgagct ggcgtgggac cctgaagttc atgtacatcg
    13261 catcggtcgt acagctcgtg caggaaatag cggtctggcg atcagtttct gtgctccgga
    13321 agaagcacag cgggccaata tcatttctga catgttgcag ataaaactta actggcaaac
    13381 gccgccagct aatagttcca ttgcgacgct ggaagcagaa atggcaacgt tgtgtatcga
    13441 tggcgggaaa aaagccaaaa tgcgcccggg tgatgtatta ggtgcactga caggagatat
    13501 cgggcttgat ggcgcagata ttggcaaaat cgccgtgcat ccggcgcatg tctatgtcgc
    13561 ggtccgtcag gctgttgctc ataaagcatg gaaacagtta cagggcggga agattaaagg
    13621 aaaaacgtgc cgggtgcggt tattaaaata atgaaatgtt gaattgccgg gtgcaagagt
    13681 aaacatctta ttcgggattg ccggatgcga cgctggccgc gtcttatccg gcctccataa
    13741 gagtagcccg atacgcttgc gcatcgggcg ctatcctggt tatttcactt caaccacatt
    13801 cagccgtaac tcatccaact gattttcatc ttcttctggc tgccagcccg ccggttgtag
    13861 tgggatctct tcgcgatcaa acgccagatc acccccgtta accacttcag aaccgtgggt
    13921 gatgcctttg aaatcgaaca ggttggtatc gcacagatgc gacggcacca cattctgcat
    13981 cgcgctgaac atcgtctcga tacgccctgg ataacgttta tcccagtcac gcaacatgtc
    14041 agcaatcacc tgacgttgca ggttaggctg tgaaccgcac aggttgcacg gaataatcgg
    14101 gaacgctttt gcatcggcaa atcgctgaat atctttctcg cggcagtagg ccagcggacg
    14161 aataacgata tgtttgccat catcgctcat cagtttcgga ggcatacctt tcatcttacc
    14221 gccgtagaac atatttaaga acaacgtttg caggatatcg tcacgatggt gacccaacgc
    14281 gatcttcgtc gcccccagtt ccgttgcggt acgataaagg ataccgcgac gaaggcgaga
    14341 acacagtgag caagtggttt tgccctctgg aatcttctct ttcacgatac cgtaagtatt
    14401 ctcttcaaca atcttgtact caacgcccag cttttcaaga tactcgggca gaacgtgttc
    14461 cgggaagccc ggttgctttt gatcgaggtt aacagccacc agcgaaaaat tgattggcgc
    14521 gctttgctgc aaattgcgca gaatctccag catggtatag ctgtctttac ccccggagag
    14581 gcaaaccatg atgcgatcgc cttcttcaat catattgaag tcagcaatgg cttcgcccac
    14641 gttacgacgc agacgttttt gtaatttgtt caggttgtat atctctttct tcgtattctt
    14701 ttgattttct tgcattattt cagttctctg gtactaaatg gggcaaattg ggggcaaact
    14761 ttgcaactac gataaccgcg cattcaacat agctacctgt tcgtcgttca tgtcatcaat
    14821 ccacataccg taaatttcat acaccatctg cgcagtttca tgccccatct ggctggctat
    14881 aaatgccggg ttcgctcctg ccgtcaacag ccagcaggca aaagtatgtc gcgtatggta
    14941 cggattacgg cggcgaatac cagcacgttt tactgctgca ttccatctcg cacccaaact
    15001 gcttaccgag taataaggtt tctgttttcc gttacacatc ctgggcatga aaacaaaatg
    15061 cagtttttgc ttttcggttc tgccgtactc ccgatgataa aaagtgattt cgcttttgcg
    15121 atgatgcccg gtcagtttgt attgctcctt cagtgcttca agagctggct gtagtaatgt
    15181 taccgtccgg atcccggcat ttgtttttgg gggaccgaac atatcaagta tcgtcaggtt
    15241 tcttctgaca ttcacaattc ccttctcgaa atccacatcc tcccacgcca gagctgccag
    15301 ttccccgtga cgaagcccgg agtaaacggc aaatttccac aagttttggc tctgtccttt
    15361 ttcactttcc attaatgcat tgaattctgt tttagataac gggtcaggct ttattctgtt
    15421 tcgctgtaat ttttttactc cttcaaatgg tttggttgat ataaatcccg actgatacgc
    15481 aaaacgtaac agcgaacaga gcagggcgat atagttatca actgtgcgca cggttcttcc
    15541 ttttttgttg gatcttggat tatccaggta aagcgtttct ccatgcagca gttcattccg
    15601 gtagtttaag atatcgctat aacgaatata tgatatcggg gtactttcac aaattattat
    15661 tctgagtgtt tttaattgtg atttcgtttt cttcattgtg tttgttgtta actctgtctc
    15721 tttaattttt gtccagatat cacaaagctc cccgaacgtt tttatgactc tcgttgtcac
    15781 catttttgcc ccagtgctgg actggggaaa acgtcttaaa tactcaaatt caccggagtt
    15841 tatttcatga actatcagcg ctcttaaatt tccggctttt ttaatattac tgtttgtaat
    15901 ctcccagcct tttaatgttt cccgacatcg ttttcctcga aacatgaacc agatgcgaat
    15961 gtttctacct ctaatctcga cacctgttgg taatttagac atatcatgag tctttgataa
    16021 actgatttat ctttggatag ttgtaccaga taatccctcg tttgctgtct ggcttaccta
    16081 aaggagatac tcgtttgaag tggaagccct ccacccaaca gttctggcgg tatgcttcaa
    16141 tttgtctggc ccccagacca gtgcgaagca tcaggccgta ttcaaccatc cactcttcat
    16201 taaagattac ttgtgccatc gcatcacctc tggcaggcgc caatgttaga ctgaaattga
    16261 cgcctgatgt tgattattaa taatcagcta tgaagtttta atttgaatac aatgcaattc
    16321 acgaggactg aagtttctcg caattaaaat ttatcagttt tactttctgc tctctggaaa
    16381 cgcctgcttc ttttttacct gagagcattt tttcgcattc tgatttcgtt agtttagatt
    16441 ttgaatatct tgtccagtta gtaggagtgc caccttcctt ttcaattgta gcggtaattt
    16501 tatacatgaa cgcctccatt aatattttca gtggttcgtt tattccatct ttcgagcgct
    16561 tctttttcac ttccaccata gccagttcgg gattcgcatc cgttgcattt tgcccggtaa
    16621 tatcctgaaa tgtctttcac cgttactgat ggacaaccac aaaacggaca tggtttaaca
    16681 tcgtcatatc tcaaagtttt tgtcataaaa actatctcac gttggcggtg cattacaccg
    16741 ccaggctgga ttattcttct ggattatcga ttacactgta ttccccggct ataaccgata
    16801 tgtcgtctgg attaattgtt tccacctctt ttccatccat cgatactgca cgctggattt
    16861 caatagatac cggcagatac ttgaacagtt ttcgtatcac tgtctttttg gccatgtctt
    16921 caaagtgttc atcccagatg gacgacgccc cttttgacgc tgcgtttttt gccgccttgc
    16981 tgtgtgtgtc gcgaactttt tctacttgtt tgcgggtcat gacttcaaac tgcactcctc
    17041 cgtctttcag ttttgcaaca gcatagacat gggttatagg ggcatcttcg ttttcaccgg
    17101 gacgatgaac cagtttttca tcaaggccaa gttcatagct gaattcatca cattcacgga
    17161 caacacgagc tgacagactg atgatttgac ctgatcgacg ggcaaggtcg atcatgccgc
    17221 gataaccgat gatcagctgt acgttcttct taccgttttt tgcttttccg ttgccgaacg
    17281 gtagcagata tgcatgaccg agggcgctac ctggctcaag tccgagctgt gaacactgta
    17341 cgatggcacc gataaaactc gtcgagtcac agtttcttag ttccggtact ttacggattt
    17401 ctgttgtagc aatgcggatc atgcgttccg ctgtcatgtg acgtggcaga gctgctgcca
    17461 gttgcgcttt cattgccggg ctgttaatca cgcacagcac atccttatcg ttaactgctg
    17521 ctggtgcacg gtttccctga gttttttgca gatcggcttt tgcgataggt ggttgcttag
    17581 tcatttgcat actccttagc ccagcggggc agtgataacg tcttaatagc tggccattca
    17641 tcggtattta ggcagtcagc cagggtccgc agattgcggt gatattcctg ctggcctgcc
    17701 agttttgctt cttcgcccat catgaaaatc tcaaccggat aacgtccgca ttcaacagtt
    17761 gtgctggcaa ccagaaaaac gaaagttggc tgcacaccaa actgtgcttc ataaccgtca
    17821 ctgtagaatg catcctgaac gtgatagcgg tagtcgtaat aagacgtttt gaatcgttga
    17881 atatccgctg tggttttcac gtccatgatc cagtgaaatt caggaataat tttgtccgga
    17941 cggcaccgac acaaaattcc tgtttccgga tcttcccagt aaattgatga ttcagcgtgt
    18001 ccggcgcttt caacaagcca ttgccccagc ggcaaagcca taacgctttg atacatgagt
    18061 tcaattttcc ggccttcttc cgcagtgata accgtttttc ctgtgcttgc gcattccatc
    18121 agaaacgctt tctcttcttc ttttccggcg tttgtacggc ggttaaattc aggtgctacg
    18181 ataaagcggt tactaaattc ttccggttca agtacccggc agtggaaagc ggttcctaaa
    18241 tcgagcgttt ttgtctttgt agtgtccacg ggggcatttt tacgccacaa atacagtgcc
    18301 ggagtatcag caatgtcatc gagctgagac ttactgacac cgggacccgc gtggtaattc
    18361 tcattcgaaa ttccgtaata aataccgggc tctatgtctt ctacgattac gggatctgcg
    18421 acttcgccag tttcatcact gcaatcgcga tgcggatcgc tgccagcatt ctcattgtgc
    18481 ggatgttcag cgccttccat ttcctccgga tctttttcct tagcttcaac ctgattctct
    18541 tcaccgaatg tttcctggta tgttgcgtcg cccatcaccg caccacagtc agggcagtta
    18601 tccccgccag tctgaccgca ggcattgcag actatttcct gttcctgttg cactactggc
    18661 tcaggttgtt tcgcatccgg gctgattatt tccgtttctg gctggttctg gtacacagaa
    18721 tcgcgagtct ggatcccctt tacccatttc ggatcgttcg ggtcgctaat tccgtcaaca
    18781 aattcaccac gtgatgcagc aagcaattta tcggcatcga caggatcttt tgatggaatg
    18841 ttttttcggg cttcatggag ttctgcccgc agttcctgat atttcgcatc aacagaattt
    18901 acctgtgact gagcatccag cggctgcgtg tcctgatgat gttcagttgc gtccggttcc
    18961 attgtttcag cctctccctg ttcaactgcc gttgttccag atggttgcgg tttttcttca
    19021 tcatcctgtt ttccttcttc tgttactcgc tgcggcatcg gggcagagga gcgaccgcag
    19081 gcaatatcca cgatttccgg atcagggttg gcatgatcgg tttcagtcag tactttgttc
    19141 agatattcag tgacgtgcgc ggggatgacc tcgatcccaa ttggtgcttc ttttacggac
    19201 gcaaccacga tggcgcggga ataatccagc ccgccaggca tggtgatgaa tttgtcgcgg
    19261 aaaacagaaa agggcggttt attttcagcg ataatttcct caatgcgttt agcgtgtgcc
    19321 ggatgaaggt tatagatgtc cagatccatt gaacgggcca gtacgccagt ggctacgtcg
    19381 cgcgccagtg acgtcagatc gtgtacgaaa ccttcgccgc gatcggtgag gtttccgccg
    19441 ccagcattag caccggaagc cgtgcgagtg atgtgtgaaa cacgattacc cttcatccac
    19501 tcttttgtca gcagtcctcg atcggtgtag tcagcgttca ggtatgcttc gaaaaaagca
    19561 gttatcagtc ccaggtttga attaccagga ttagggaaaa ctttgtcagt gtcacgaacc
    19621 agtttgtgga gttcgcgaat ttccagcggg tcgagcaggc tggttttgtg ggaaacagcc
    19681 agggcagtaa cagccggtag ttcttcagcc cgagcaatgt gtaatgcctg gagtccgtcg
    19741 cgtgaaacgt gcgttaccgg tttttcgctg ccgtgttgag caagccaacg aatgggcagt
    19801 tcctggccag aaattgggag tagcatattc tcctcaatct cagtcatgtc ttcgccgttg
    19861 acgttggtat tgccttgata gtgagcgttg tctggtgctg ctcccggttt tagttcccat
    19921 gtcatggagt ctttgctgag ttgatagcgt tcactccagg taaaatcgat ctcaccttca
    19981 gcgggcaggt cattaacgac aggaaaattc gtggcaacag ctttaaaata gctgctcagt
    20041 tttttacctg acttaacgat caggtagtcc agagtggcac aggtcgattc aaaatcgttg
    20101 cttgcccaca ggacgacgtc aggttcaccg gatgattttt tcgctttccg taacaggaag
    20161 agtggttttg tgctcattgt tttttaacct caactcagat taaaattcgt tttgttcagt
    20221 gaatgatctt gccggataca cactgttcat agcctgcgcc atacgcaggc tatttctttc
    20281 agatttcacc ttttaatttc attgcaatta gagttgccag aaattcggct tttttttctg
    20341 cgggcagatt ctttccgata tgcaccaggc acattttttt gacaccttca tcaagtgttt
    20401 ttacgttgcc tgatggacca tcgatatcaa ccacagtgaa tggggtttct ttattttctg
    20461 ttttaattac gtagccaatg cgctttcctt ccagattcac ctcgtgaaca atgtcatcgg
    20521 tagttacaac agtggcttca taattggtaa tcatgttttt ctccttaatt aaggttgagc
    20581 gaatacctgc catttctggc ataaattcag tttcgaatag tcaattaatt aaagttcatg
    20641 tgccatctgg tctttttcgg cacaagcttc actgcaatat tttctcggtt cgtcttttga
    20701 taaaaccccg tgcatgaagt gaagcattct ttcaatagct ttgctttctt caacgtcttt
    20761 tttgcaaagg tggtaagcac attttatttt cttagtcatc accatgactc cgcctttaca
    20821 ggtaaaccat cacgaccgag gaagacttta atcatgcggt cagtaatgca tgtttttgtg
    20881 gtcaggttac gaatatatag ttttcgcttt ttaatattgt ttgccgaggc aatatatgtc
    20941 cggccttcat gaagaacata atcgccagga gtcacacact gacgtggtat ttcatcagtt
    21001 ccgaagtgat gtgcaatcat aattatctcc atttttacaa atgaactttg ttgatgcggt
    21061 gcctggtgcc tccaggtgac tgcaaccagt taacaattac agtcggcttt cccacccaaa
    21121 ccaataagga ctaacatgac ttttaactgt gccacgtgcg cttagccgca ttcaccgcat
    21181 cacaaaattc actttaaaaa gggcggacat cagccgaact tcaagaaaaa aactgatgcc
    21241 gccaggacta cacacagcaa tgtcgttatt cacaaccgga ggcgcactcc caccatttat
    21301 atttaacaga caagaccgac tctttatgga tatcggaaat gcgccttcgt gttgtgcccg
    21361 gttttatttc accacctccg ggcttcggtg gtctcggcta tacccctaca gcgagagctt
    21421 gtgttaacat ttcaataccc ttacagtcga gagttattga aatgttggat gtatttactc
    21481 cattgttgaa actttttgct aaagagccac atcgaaagac ttatgtacac gattatcatt
    21541 tttggcctca ctctctggct gataccgaaa gagtttgctg tcgtatttaa tgcttatact
    21601 gaaataacct ggctctttca gattatagtt tttgcctttt ctttcgtggt tgccatttcc
    21661 ttctcaagat tgcgagcaca tattcaaaaa cattattcat tgctaccaga gcaacgagta
    21721 ttgcttcgtt tatctgagaa agaaatcgct gtatttaaag atttccttaa aacaggaaat
    21781 cttattatca cttctccttg ccgtaacccg gttatgaaaa aattagaacg gaagggcatc
    21841 attcaacatc agagtgatgg cgcaaactgt tcttattatc tcgtcaccga aaaatactcc
    21901 cactttatga agttattctg gaacagtagg agcagacgtt ttaatcgtta gcttactgtg
    21961 tacttctcca accagcgacg cgcgccaact tcggttttaa acgttttgct tttggtatac
    22021 gtcatggcgg tgaacgttcc atcctggttg gggaacacgc cacacaccag ggattcgttg
    22081 ttgctaagat cgatagtatc catgttgacc tcattttccc ttaacgctgg gggaattggc
    22141 ttcatagttg ctccgtatcg tggagcctac gacctgcttt taaccacatc aggtgaggtg
    22201 atatccttac aaatggtaat ttgctgtaag gatattaatt atggatatta aatttcgctg
    22261 tcctcattgc cgatctcgct acattgagat cgatgagcac tctggctctg acaatgatcg
    22321 ttttatctgc ggtgattgtt ttgcggaaat caaagattct gatttcagtg aacactttaa
    22381 taaaacaatc aataaaatca tccggacagt taaaaaccgc aggaacaagt agtttgtata
    22441 acttctcaat ctgatgtctc agggggctgg tatctatttt taacaccagt ccctgctttt
    22501 catctgcgat catcttcgta tccccttaac gccgggtggc ggaactgttt gctgagaaca
    22561 ccgtgcggtg ttttgatggg atgtaattta gttttctcat tgtttttggt caagtgtttt
    22621 tgatgagaaa actcaatatt tgactcaaga taaagccaat acattgaaat gtaaggcttt
    22681 aaaaatttgt gatggggtga ttattgatgt ttgttacgct tgcgagcttc taatagctca
    22741 gtaaataggc gattaaaatt ctcaactcga gcgctgagtt cactgatttg tgcttgttgt
    22801 tcggattttg gaagtgcgcg atacagtcgc aacatctcca gttcatcttc cgataagtct
    22861 aaggggctat tgagtgaaac tggggcatcc ggagttttat cctcatcacc gaacagtatc
    22921 caggtcggtg agcattgtaa tacctcggcg agtctatgca aattttgccc acgcggggct
    22981 gtatggtcac tttcccataa tgaaattgat gagccagaaa cgccagcagc tttgcttaaa
    23041 ccactttgac ttaagcctac ctgcttgcgt ctttctctaa ttcgttgacc taaagttttc
    23101 tcgttcatat ttagatatct taataaccct tgacttgaga ttccttgagt gattaacatt
    23161 gagaaaactc aatattggag gtgcaatgtt taaatcagac gtaattaatt attacggcac
    23221 gaaagccaaa gtggcgaaag ctgctggcgt tgatccatct gctgtttctc aatgggggga
    23281 actggttcct gaaggccgcg cgatgcgtct gcaagaggca tctggcgggg agcttcagta
    23341 cgactcaaat atctatgaca aatatcgtaa ggcaaagcgg gcgggtaagg gtattcatga
    23401 aaatcaggca tgagcacatt gaatcagtgt tgttagctct ggctgccgaa aaaggtcagg
    23461 cgtgggttgc caacgcaatt actgaagaat atttgcgcca gggggagagt gaattagccc
    23521 tggctccagg caaggactgg aacaatcagc aaaacatcta tcaccgctgg ttaaaaggtg
    23581 aaacaaaggc gcaaagggaa aaaatccaga agttgattcc tgcaatcctg gcaattcttc
    23641 cgcgcgaact gcgacaccgc ctcagcgttt ttgacaccct ggaacgtcgc gcattactgg
    23701 cggctcagga agcgctgaat acggcaattg atgcgcatga tgatgcagtc caggccgttt
    23761 accggaaagc gcatttcagc cgcggcggat catccggcga ttctgtcgta gtgcattgat
    23821 tgaaattaat cgtgccggag cgttttgttc ggtatcagtt aaatgtaacg ctgcgagcgt
    23881 tacaaggtga aaacaatatg gcttcaaact ggataaagct cgaagttatt acgccggata
    23941 aaccggaaat attcaggatt gctgagattc ttaatattga tccagatgcc gcattaggga
    24001 aggtcattcg attctgggca tgggcggatc aacaaatgat tgatggtaac gcagagtgta
    24061 acgctcgagg tgttacaaaa agtgcaatag accgtatcac ttttatggct ggttttgctg
    24121 atgcgttaat tcaggttgga tggctggtcg aaagtgatgg agtgctttca ctacctaact
    24181 ttgagcgtca taacggaaaa agctcgaaaa aacgggcggt tacaaacgag agagttacaa
    24241 aaatacgcga actgaagcga aaaggtaacg ctgccagcgt tacacaaacg gatcaaaaag
    24301 cgttaccaga ggaagaggaa gaggaagagg aagataaaaa tactgatctc cccctaaatc
    24361 cccctcggca aaaacgagcg tctaaaaaat tcgagccgga ggccattgct ctgcccgact
    24421 ggttgccaga aacactctgg aatgagtggg tccagttcag gcaggcattg cgaaaaccga
    24481 ttcgaacgga gcagggcgct aacggggcga tacgggaact ggaaaaattc cgccagcagg
    24541 gttttacccc tgagcaggtg attcgacaca gcatcgccaa tgaataccag ggcttgttcg
    24601 cgccgaaagg tgttcggcct gagacgttgc tccgacaggt taacaccgtc tcgttgccgg
    24661 acagtgcgat cccgccaggc ttcagggggt aactgaccat gaaaaatatt gcgacaggcg
    24721 gcgttctgga acgtatccgc agactggccc cgtcacatgt aaccgcgcca ttcaggacgg
    24781 tggcggagtg gcgcgagtgg caacttgcag aaggccagaa acgttgcgag gagatcaatc
    24841 gccagaatcg tcagttgcgg gtggaaaaaa ttctgaatcg ctccggcatc cagccgttgc
    24901 accgcaaatg ctcgttttcg aattaccagg tgcagaacga cggccagcga tacgcgttga
    24961 gccaggcgaa atccatcgcc gatgaactga tggtcgggtg tacaaatttt gcgttcagcg
    25021 gaaaacctgg taccggaaaa aaccatctgg cggcggctat cgggaatcgc ctgctgaaaa
    25081 acggtcagac agtgattgtg gttaccgtgg ctgatgttat gagtgctcta cacgccagct
    25141 atgacgacgg gcaatcaggc gaaaaatttt tgcgggaact gtgcgaagtg gatctgctgg
    25201 ttcttgatga aattggcatt cagtgcgaga caaaaaacga gcaggtggta ctgcaccaga
    25261 ttgttgatcg ccggacagcg tcgatccgca gcgtggggat gctgacaaac ctgaactatg
    25321 aggccatgaa aacattgctc ggcgagagga ttatggatcg gatgaccatg aacggcgggc
    25381 gatgggtgaa ttttaactgg gagagctggc gcccgaatgt tggtcagcca ggaattgaga
    25441 agcaattttt accgggagga aattttaatg gagaccgttt ttgacgcact gaaagcaatg
    25501 ggaaaagcct cttcccagga agtggcagca cgtctgggaa tgacccggga tgaggcgatt
    25561 aacgagctgt ggaaactgaa gcgtcgcggg gaagctgata acaagggccc gatgtggtgg
    25621 ttgactcaaa ccgacgaaaa cgcatctgtg gcacaggctt ctaaagtgac agcgcaaatg
    25681 ctgatcgagg cgattgaaca gcatggccct aaagcggctg atgaacttgc actgatattc
    25741 agaattactt cccgcagggt gaactcatcg ctggctatgg ccatcagcaa aggtcgtctg
    25801 attcgcgtaa atcagaacgg taaatttcat tactgcctgc cggacggtaa tttaccagca
    25861 gagccgaaag ttgtatcggt agttaaaaca cctggtaaag gctttcctca gccagccggt
    25921 gttgcgttac cagtacagga agcggcaaca caggaagaaa ttaaaacaga tactgtgggg
    25981 gacattgtgc agtcactacc atcgttcact gaaacgcgag ccaatgacct gattttacca
    26041 tcgctgcaaa tggcaaaccg cgaactgcgc cgggcgaaaa gtcatgtcca gaagtgggag
    26101 cgagtctgcg ccgcgctgcg tgagctgaac aagcaccagg acattgtccg tcagattgtc
    26161 gattcctcca gtcgtattgt gtcggaaaag tgattccagg ggagggctta tggcaaaagt
    26221 attaacacag gaagagcgag aaaaaattaa agggcagatt gttgatctcg tacgccagag
    26281 cgggcgagag acgttacgac aactggaagc taaaacaggg gcaaccagat atctgataag
    26341 tattctcgcc aaagagctgg tttcgagtgg tgacgtatac aactctggct atgggttatt
    26401 tccgtctgag caggcacgtc aagactggca aaacgcccgc aaaaaactct cgagggcaaa
    26461 gtcgaaggaa ccggttatgg ttgatcctga ccttatctgg tcattaccag acggagaaat
    26521 acgccgctac gacaggtgtc agaacataat ttgtcgcgag tgccggaaga gtgaagttat
    26581 gcagcgtgta ttagcatttt atcaggggaa tgttggtatt tattgaagtg acgagattaa
    26641 agaccactaa ttcatatgtg aattgacagt ttcatggcac aggatacggc tagcgtggtt
    26701 gtctgttttg tgccaggggc ggaccttaag cttcagtaat ttgattagca caatccctat
    26761 gataaacatg cagttgcagt gttgctcatg taaggcgcgt aatttcaata ggacatccaa
    26821 acgttagtcg gaaaaagccg actaaaaaaa ctgagtggac aagtagtgtc catttaatta
    26881 atgccatttt gagggtattg ttaaccgtgt gcataatgtt ctgatttata atacaaagag
    26941 ccttatcatg ttaagccatt ccgatgtaca tcaaaccatg attacaagag aaattatgac
    27001 accgccaacc aaaattgata cttcaagtct acttaccata ctgggcgtta ttgctgccgt
    27061 atgggccctt attactccaa acgctcgact acgtttgagg ttctgcctgg cgtggtggga
    27121 ttgggcaatt gttggtttcg cattcttact aagcaattat ttggtgtttg caccagcttt
    27181 aaaatcacta gacttatatt ttagttttgg cccctggaaa tggggattag atagttcaag
    27241 tgccgtatac ctgatatcac tcgcagttgc tatttacatt ttatttcgct taaaaaaacc
    27301 taaactttca gttggaagaa caggaatatt tctagagtta gtggaaaatt tgcaccttac
    27361 taaacgatac gatgatctgg cacaattgtt ggcacctcaa ctagaaaagt tgatatcaat
    27421 aattgatagt ccggtgaaga atcgactttg tgataagata gctaacaatc tccgcatctc
    27481 taatcgcgag actgctgccg aatatgcaca tgaagcatta ttaaatattg tctcatcacc
    27541 tgaacttacc aatcactttg cacttgcgca cccatccctc tgccttgagt taatcaagat
    27601 cgaaccaata gttcgttctg actttacttc taattttatc agtgcattac tagattcacc
    27661 taacagtcgt ctatatgttg agcttaaaaa taatcttaac gttagtcgag ggcatcgttt
    27721 gttgatcccg aaaaacaatc gtatattaca ttttttcttt tccaatgcta agtttgcggc
    27781 tgatttagca atataccgtg acatcggtga atatatctac tggaggttag atgaagatga
    27841 aaaaattatt gctacactta ataaatcttt aggttcttat tcagatgttt cgaagtataa
    27901 atgtcctatt tactctggga tcacgctgtt tgaaatcatg gttcatgaag gtattcatca
    27961 ggggcttcag gatcatttat ggctgcatta ctatacacat tttgcaaaga aaatcataaa
    28021 gaatatgaat aggcagtcga atgaatatag tggtgaatgg gaaacacctt ttcacttcct
    28081 tctctgtcat ttatttagca tcgctataaa ttgggcagaa caatgcgaat ggatagatga
    28141 aagggacatc cttcaagaaa ataaagaaac agaaaatttc gatcttcatt atatttcaaa
    28201 agaggctacc aaattacttg gtgctatgtt ggagttggtt ttgcctaatt caaaactcac
    28261 tttgaaatct aggaaagata ttcttggtat aattgtgtct tgttacatta gattgaaaag
    28321 gaataaaaaa ttaaaagacg tcgctgactc attattaatt ttcacaacta gaggcgaagg
    28381 taatttggca tcgccgtatt atcgtaaaga acttctagaa atattcaata cacttgatga
    28441 ttatcgatta agaagtgatg ctccggaatt ccgagaagct atagagtctg cgattcaagc
    28501 aaggcctaat taggtaaatc aaccacctgc atggcgggtg gtttagacat agacctctaa
    28561 gtacgcttgg cagaggtacc agtaaacttg ataattggag ttgttctcat cagtcgttat
    28621 gaaaaatttt gaacttctgc ttttcgctgt gagttaaact gatggacgca agacacttat
    28681 tgaaccgttc tgcgggtgat tcgtagtcta gcgtttttct tggccgctcg ttgagctgtc
    28741 tggcaacact gtgtgaactg ataagtcagt tccttttgga aaatactgtc tgagtaatct
    28801 gtttgtattt tcatttgagc cgcgttgcca tggagattga tgatcacaga aataaatctg
    28861 aatatctgtc gctacagtaa accgggtatg gctggtcatt tcagcgcccc ggtcccatgt
    28921 tagtgtttta taaagctcaa caggtaattc ccgggcttgt ctgatgagtg cagatataac
    28981 cgttatggtc ttgttgtctc tgatttttgc cagcataaca aagcgggaat ggcgttctac
    29041 gagggtgacg ataaaggagt ttttcgagca ctggatcagg tcaccttccc agtgacacgg
    29101 tatggctctg tctgcaactt caggtggcct ttcgcttatt ggtatcgtat ctgggattga
    29161 ccctaaccct ttccctttaa gcgatgatgt tctggatcta cgaaccgctc ttccgcttct
    29221 gaggcattgc tgcagttctt tttttaatgc accccgggtt tgtataaaaa gcgttttata
    29281 aatcgtttcg tgtgacacat gcatttcctg attatccgga taacagcgtt tcagccagcc
    29341 ggcgatctgt tccggcgact agtcctgatg catcttctct gcaatgattt tacacaatgt
    29401 gggctttcaa ttagcttgca aggttttggt ctcagagcat tttcccatgc agcagtatcg
    29461 gcttttgctg cacggtattg tttggcacct ccgtgcctcc tgacctcgcg gctaatcgtt
    29521 gaaggcgctt ttgataattt ggcggcgatg tccctgatac tgagttttgc taccactcct
    29581 ctggatatct cctctctttt atcaagcgaa agcgctaatc ggtaccgttt tcgcacggga
    29641 ggacggtagc tccctgtctg gtggatagtg ggcataatgg aagaatgata tctgtcgaac
    29701 attctggcga tatcatggag agaatcacct tgcttatatc tgtcccagat aattgccttc
    29761 tgctctggcg tgtagttaat ccgagttctt cgtttcatgg caacgtcccc cttgattaag
    29821 gatagcgttg catccactga ttgaactcac agccggaagc ggtcattcta ggtctttcga
    29881 ggaatagtga gcactatatt aatatctggc ttatatctga gcacctaggc gcttgaaaga
    29941 tgtgttaccc tgcgattata cagtgcgaga ttattagtct cacttataaa tctctaaaat
    30001 ttaaaggtgc aatgtaatcc tctttatagt agattttcta gtatggggaa gccacgtttt
    30061 aagtgcggtt tgaccaattt aatgagcgtt ataactcatc aataatagtt acatggagtt
    30121 gtttaatgtt tttatttttt cactctgaac aaactgaaaa aacactagtt attacaaggg
    30181 gttacttttt gaagagttgt taaaagaata cttggaaggc tctggatacg acattgagtt
    30241 aaggaaaaaa gaatctagtt tagagtacga cattgaagga ttctctagag caactggcca
    30301 aaagattatt ggcgaagcta aagcacacag caaaagcatg agtggacaaa cagttgctgc
    30361 gttcgttgga aagcttttgc cgctaggtct tctggaaaaa aaggtgcatg gattatttct
    30421 gtcattatca ccattaacac cggaggcgga agattatttt cgaactgtcg aaaatatggg
    30481 gttaacgaca aaggtaggaa aggaacttta taaccaaata actgcgactt tgcatttgcc
    30541 gactgaaaca tctctacata aggaaattaa aggttatgac cttgtggccc caaacattct
    30601 caagactgat acggggttgt ataagttatt tattttaaaa gaggtaaatt caggaacgcc
    30661 atcaaatttt gtggtattca ataaaagtgg cagccaaatt gatgatgaaa gatttttgga
    30721 aaatttaaaa atagggattt cagaacttgc tggtttggat ttcattatgc ctagcaagaa
    30781 aaaaatagtt gacgaaatgc ctcaagtaaa gcgtgaaata gttcgaggat taactgttgg
    30841 gactgaatgg gctgactata ggctacctgc agggccaact gtgttcgttg gcaggactga
    30901 attcataggt gaacttcttt ctcacatcaa acataatgag ctaccacatg ttcttcagat
    30961 aaaaagtcgc tctggagtag gaaaaagctc tcttgtatcc tttttagaaa acaagctctc
    31021 aatggatggg gttattactg aattacatga ctcaagggat gtaaaaacaa tatatgatgt
    31081 tttttatctt gttcagaggt ttacccaaag cagcattatt gcgactaatt ttatagaact
    31141 tgatgaacaa ctgaaagact tgcaactttc actaaatggc caaaaggcag ttttctttgt
    31201 tgaccaattt gaatctacat tctcgaatcc tgacattttc gattgctatg aatacattgc
    31261 gaactcaata acaaagcttc gtggtggtgt atacatagtg tttgcgcgta aaaatgatca
    31321 gctaacaacc tatgacaaca gtaaagtttc tcttaataga attaatcagc tatcgaaaag
    31381 ctttactctt ccggattttg aaaacaaaga atcaatactt cttttagaaa aaataaatga
    31441 aaataactct aatattctca gtaaagaaat actaccctat attattgaat ttgcgcaggg
    31501 ttttccttgg cttttaaaga gaacgatagc tcatgtttta aaactcactc gaaaaggtga
    31561 ctctcagagg gatttaatca gtgcgggctt aaaactgaac gacctttttg aagaagagct
    31621 tgaaggtttg gatgagattg aaaaagatta tctagttaag attgcagcta agctaccagc
    31681 agatttcaat gagcttcaat accaatttga cgaagaccca atgcttgtaa aagtcctcga
    31741 taagttaaca gcttacagat tggtacgctt gagtggttct acctatgata cttataacga
    31801 tgttttcaaa gaatatcttg tctaccaaaa gcttcctgaa tttagactgc tggttattta
    31861 tagaatgtat ccaagctctg ttatatccca gtttcataaa ctgataacaa tgagaaagct
    31921 gacaattaca aaaataacgg aaaatctaaa tgtaagggaa ggttacgcat ttaatctcgt
    31981 taaggaattg cggaatttag acttgcttga tgcagttaaa agtggtgaat ggataattcc
    32041 aaccaatgtt agggatatat attctcaagg aacccttgct ggttatctac gccgaaaaat
    32101 tttagacaat caactcgtcg agactttact taaaaaaatt ctttctggag aaaaagtttt
    32161 cgagagcgat atacccacgt atttttctga gtcttatcct tttattgagg catcagagaa
    32221 aacatgggga ttatactcta cgattttaaa gtcatggtta cttgccctaa aaattataga
    32281 aattgatggg gaaggtcgca ttattgcctc agatgtgtcg cataatgatg caacagctga
    32341 gttaggaaat cttaagaaaa tttcgggctt aagggcagga aataaagggc ttttcttccc
    32401 tgtaacatct cacgctaaaa tgattgaagt taccagcttg atactgaacg gtaaagagcc
    32461 tactggaaaa gaagcgctta aagcaaaatc tgatttaaat aatgctggtg ttttagtcga
    32521 tgggcactta gcagtatcta atattgaaga gcttaatgag gcacttacag ctcaaatact
    32581 tgatgacggc tatgaggatt tctggaaagc aatacacgct ggtcagcctt gttatgaaat
    32641 ttttaaaggt attgcagggg aaggattaac tgagtccacc cttaagtggc gcttaagaaa
    32701 gttaacggct ttagcaaaaa aactgaaaat cataccaaac aaaagattca aatattaatt
    32761 actgtcataa cattaaaaag tagcggatgt attcctccgc tacataactt atactcaagg
    32821 agtgattata ttgaatattt tcaaagcctg tgggggcatc aatgtccgct cttcgctcaa
    32881 agcggactaa aatgttggct tgtgtctgac tcagcgcatt taggaagtgc tggtggtgac
    32941 ggtttgttgt gttgcacaga aaaaaaatag actaagtaca ccaaatagtt ttattgagtc
    33001 accagtgaga catccacata tttatcagca catgcgagtg tcaaatgcag gaggttaacg
    33061 tgctggttac gaaatgtgcg ttggctatag cggctgtaat ggcaatctat ggtcttgcta
    33121 ttgctcttat ggatcacctt tctgattgat ttcatattgg cgaggtaacg ggagttaagt
    33181 agaatagctg cgggtgcttg aggctgtctg cctcgggcat gaacaccaaa ggcagataga
    33241 gaaaagcccc agttaacatt acgcgtccgg caagacgctt aacattaatc tgaggccaca
    33301 tctatgcttt gcatacgtag attagcctct taccgaccaa aaggtcaagg agaagcaggc
    33361 tatgaagcag caaaaggcga tgttaatcgc cctgatcgtc atctgtttaa ccgtcgttgt
    33421 gacggcactg gtaacgagga aagacctctg cgaggtacga atccgaaccg gccagacgga
    33481 ggtcgctgtc ttcacagctt acgaacctga ggagtaagag acctggcggg ggagaaatcc
    33541 ctcgccacct ctgatgtgtc aggcatcctc aacgcacccg cacgtaaccc gcttcggcgg
    33601 gttttgtttt ttcctggcat tctggtttac aattcgcacg ccagcctgaa caactggcac
    33661 ctgctgcgcc agcagagaca accgatggcg cacgatacca aattacacaa ttctaatgat
    33721 tctgccgtct ttgccagtag gtgcggacgg cgttttcacg cattcaaatc agactggttc
    33781 cagcatcctc catgcactga agagcaggcg gaatggataa ttcagtgtta ccgcaggcgc
    33841 ggatacgagg ttaagaaagc tcttagcctc gactaccgtc actggataat ctcagtcaga
    33901 ctcccttact ccgaacgccc accgcgttca tcccgcacat accagcaacg gatctggagg
    33961 taacgtgcgg atattacttc gacctgttct ggtaccggaa ctcgggctgg tggtccttag
    34021 gccgggccgt gaatccatgc aagtatttca taaccctcga gtgctggtgg agcctgaacc
    34081 gaaaagcatg cgcggtctgc cgtccggagt cgtccctgcc gttcgccagc cgctggcgga
    34141 ggataaatca ttactgccat ttttcagcga tgatcgggtg attcgtgctg ctggcggcgc
    34201 tggggcactg tctgactggc tgttgcgtca tgtcaaatcc tgccagtggc ctcatggtga
    34261 ctatcatcac agtgaaaccg tcattcaccg ttacggtacc ggcgcgatgg tgttgtgctg
    34321 gcactgtgac aaccagttgc gggaccagac atcagaatca ctcgggcaac ttgctcatca
    34381 aaacttgtca gcatggatga ttgacgtcat ccgtcacgca atcagcggta cgcaggagag
    34441 ggagttatcg ctggccgaat tatcctggtg ggcggtctgc aatcaggtgg ctgatgcgct
    34501 tccggagtct gtattgtgtc gttcactggg attaccggtg gaaaaaatcc gctccgtata
    34561 ccgtgagagt gacatcgtac cgggagaaca gactgccacc agcatactga agcagcgcac
    34621 aaaaaatatt gcgctgccac ttcacgtcca ccagcaacag cccccactcc aggaaaagac
    34681 gttagtaagc atcgccgttg atccggagtc tccggttcag tatctccagc gccagaaacc
    34741 acaacgggaa gagatgcctg tatacacgcg ctgggtaaaa acgcagaaat gcatgacgtg
    34801 cggtaatcag gcagatgatc cgcatcacat cattggtcat ggcctgggag gtatgggaac
    34861 aaaggctgat gatttgtttg ttattccgct gtgccgtaaa tgtcataacg aactgcacgc
    34921 cggggtaaaa gattttgaag aaaaacacgg cagccagctg ttgttgctga ttcgtttttt
    34981 aatgcacgcg agaaattcgg gtgtcctgaa gtggaaagca tgaatgactg aacgcataga
    35041 atttgttttg ccttacccgc cgacggtgaa tacctactgg cgacgtcatg gcaatacgta
    35101 tttcatctcg gaggccggaa agcgttatcg ccgtgatgtg gcgctaattg ttcgccagca
    35161 gcggctgaaa ttaaacctgt ccggaaggct ggcgataaag gtgattgcag agccaccgaa
    35221 taagcgtcgt cgcgacctgg acaatatcct gaaagcaccg ctggatgcgc tgacgcatgc
    35281 gggagtgtta atggacgatg agcagtttga tgaaatcaat atcgttcgtg gtcagccagt
    35341 atctggtgga cgtctggggg tgaaaattta ccccataatg catgaagagc aggtcaaaaa
    35401 atgaaactgg aagatttacc gaaatactac tccccaaaat cccctggcct gaccgatgca
    35461 tcggcctcaa cgtcaaaaga tgcgctgagt atcactgatg tgatggccgc gcagggcatg
    35521 acacagaatc gggctgagat ggggttttct gcgttcctgg ggaaaatggg catcagtatg
    35581 aatgacaggg cgcgggcaac agaattactg gcagattatg cactcagtcg gtgcgatcgt
    35641 gttgcggcgt tgagaaaact tccggcagaa ataaaaccgt tagtgatgcg cattatggct
    35701 tcgtacgctt ttgaggatta tgcccgcagc gcagcgagta aaaagcagtg cccttgttgc
    35761 tatggggaaa aatttattga aagcgtagtt tttacaaaca aggtccagta tccggatggt
    35821 aagccgccgg tatgggcaaa gtgtacgaaa ggtgtgtatt cgtcttactg ggaagaatgg
    35881 aaaaaagtca gggaggtggt aaaagttgcc tgtccggagt gtggcggaaa gggtgaggtt
    35941 tccaccgcct gtaaggattg ccgtgggcgt ggtgtcgcca ttcatcgtga agagtcggta
    36001 aaacgtggta tgcctgttat cagagactgc cagcgttgtg gtggtcgtgg ctatgaaaga
    36061 ctaccatcaa cggagacatt taatgctata tgcgaggtga caaaccagat aacacgcgcg
    36121 tcatgggaaa aaacagttaa gaaattctat gatgcgctgg tgacccggtt tgatattgaa
    36181 gaagcatggg ctgagcggca gttaaaaaag gtaactaggt aacaaggttg atttttccgg
    36241 aatctgtggt aaattcgtca taataatggg ctttttatgc ctgacgttag aagagtttct
    36301 acaacccgcc gccgagcggg ttttttattg cggaattaat tacggaccgt tattattctg
    36361 ctcccggccc tttagctcag tggtgagagc gagcgactca taatcgccag gtcgctggtt
    36421 caaatccagc aagggccacc atcacaaacc gccattagct tatcaggaag agcagacgac
    36481 acgataacag ggttgttggt gcgggggcgg gtccccgatg gcggtccatt atcggtattc
    36541 agcgttgtta gctcagccgg acagagcaat tgccttctaa gcagctgtgg ttgcactcct
    36601 gttgtttctg gtggtgatgg tggacttcag cagccggata atgtcggtgc tgtctgatgg
    36661 tgttttggtg gcgggtgtgt ggttgttgct ttcccgttgc tgaaaaagaa agcatcaggc
    36721 gattagcagg gtatcagtta cccgttgaaa tttttaaata cctcacaatt ccacagcttg
    36781 atgattgtct ggctgccgga gaatttgtta aaaattacat cgcatggtga atccccctca
    36841 gcggcggggc atctggcaaa gtgtatgatc cagagaacat gcaaattcag tagacaggct
    36901 gaatttaccg ggaggcccct ggcaccatgc gacagacaga aattaggcta tacttcagcc
    36961 cctctccgga ggggcttttc tgtgcaggat gtgtcacagt ttcctgaatt ctgagtactg
    37021 tcctgttact cagggtgcta tattttctga cgtgatgaaa gtctgccgga aggcggaacg
    37081 tatcggaaat gacccagtag agaaaacgtt gactcagata ccgatgctga gttaccggga
    37141 aaccggcatc acatgaccgc tatccttcca ggctcgctcc ggcgggcctt tttactgcag
    37201 aaaacagttt tcccgtaaaa tgccacgttg ctcataattc aggctggcga ttattgtctg
    37261 gccggcggga agtttgttaa aaaatttcgc atggtgaatc cccctgtgcg gaggggtaat
    37321 cagcgagtag gtatatggga taatcgcgga ttcaggtgct ggtactgaat tcaccgggag
    37381 gcacccggca ccatgcaatg gcacatagcg ccactctcca gcccctctcc ggaggggctg
    37441 tttatattga ttttgtcaga tgtgagtaaa ctccttatgg actttgttgt tttagtccat
    37501 aaggacatat ttgcagaatg caacggttat taaagcattc attcaatacg ttatctgtat
    37561 ttgtagggca ttcctggctg tttttgatta aattccagaa tgttttattg aatggtacta
    37621 cgttgtaaat ggttacaggt agcactttgt tattgagcat gatacctgtg tgagtcagtg
    37681 taaatatact ttcaggaggt aagaaagcat ccgattgata ccagattatt aattttactt
    37741 tactccatat gactgaaaaa gatattccgc atgatggctg gataactgta tcaatcacaa
    37801 tccacttcat ttagcttcct tgtttatgcc ttgctggtga tgttctgaaa agtataaatg
    37861 atatttttga atataaacca tagagcagaa ttatttttct gatgttgttt attgtttatt
    37921 taaatgcagg gtggtttata tctcgtcttg tagtttatcc atgcatatct gcttgatgat
    37981 caggtttcta tttaaggtat ggttttgtgt tttttctgtg ttacatgtct ggtattttaa
    38041 agaattattt ttcagatggt ggaaagaacc atggcattta aacactatga tgttgtcagg
    38101 gcggcgccgc cgtcagatct tgcggaaaag ctgacacata aactgaaaga gggctggcag
    38161 ccgtttggta gtccggtggc cataacccct tataccctga tgcaggcgat tgcagcagaa
    38221 ggtgatgtgg tggtcagtgg tgcaactgag ccggagtgat actacgtcat cgtactggcc
    38281 cggcattcca ggccataaaa gacagtctgg cagtgggact aaatgcactg acgctgacgg
    38341 atattaccaa aaatgcaacg tatggcgttg agatagaaag tctggtgctg gagataaata
    38401 caccggcatc atcataaaaa gtgagccagt caaatggaag gtatcgttaa actcaccggt
    38461 agtgtcagtg gatcgtctga gacgcttgca tgagttatca gagccatcag tagttaactg
    38521 gtggcttttt tattgttgtc agcttccgga taacgggaga cggggtatgg accagatgga
    38581 aaaaatcaca acaggtgtgt catacaccac gtcagcggtg ggaacgggct actggttcct
    38641 gcagttgctg gacagggttt ccccgtctca gtgggcggca ataggcgtgc tggggagtct
    38701 gctgtttggg ctgctgacat atctgactaa cctgtatttc aaaatcagag aggaccgtcg
    38761 taaggcggca cggggagagt aattcaatga ctcaaaacta tgaactgatt gtgaaaggga
    38821 tccgcaattt tgagaataaa gttacggtaa ctttagcgtt acgggacaaa aaacgctttg
    38881 acggtgaaat ttttgacctg gacatctcgc tggaccgtgt tgaaggtgcc gcgctggagt
    38941 tttatgaggc agcagccaga atgagtatca gacaggtctt cctggatgtt gctgccgggt
    39001 tatgtgaagg ggatgagcag tcgccggaaa agcgccccgt aattttagag gcgcagaatg
    39061 tatggataac ctacaaagga aagctaccgg gaagaattac tggttctctg aagactcctc
    39121 cggaatcaca accttaagtc actgaccgga acagataaac ctgtccgtgg gcagaaaccg
    39181 ataaatcctg ataaatatcc atgaacgcaa aaatcagata cggcctgtcg gctgccgttc
    39241 tggcactgat tgccgtcggt gcgcccgcgc ctgatattct cgaccagttt ctggatgaaa
    39301 aagaaggtaa ccacacaacg gcataccgcg atgggtccgg catctggacc atctgtcggg
    39361 gtgccacgat ggtggatgga aaacccgttt ttcccggtat gaaactgtcg aaggaaaaat
    39421 gcgaccaggt caacgccatt gagcgtgata aggcgctggc atgggtggag cgcaatatta
    39481 aagtactact gaccgaacca caaaaagcgg gtatagcgtc attctgtccc tataacattg
    39541 gccccggtaa gtgtttcccg tcgacgtttt ataagcggct gaatgccggt gatcgtaagg
    39601 gcgcatgcga ggcgattcgc tggtggataa aagatggtgg gcgcgattgc cgcatacgtt
    39661 caaataactg ctatggacag gttattcgtc gtgaccagga aagcgcatta gcctgttggg
    39721 ggatagagca gtgagcagag tcgccgcgat tatttatgct ttggttatct gcatcatcgt
    39781 ctgcctgtcg tgggcggtca atcattaccg tgataacgcc atcgcctaca aagaacagcg
    39841 tgataaaaaa gtcagtgagc tgaagcaggc gatcgccact atcgctgaca tgcagcagcg
    39901 tcagcgtgat gttgctgcac tcgatgcaaa gtactcgaga gaattagcca atgcgaaagc
    39961 tgaaaatgaa actctgcgcg ctgatgttgc cgctggtcgt cgtcggttgc acatcaaagc
    40021 agtctgtcag tcagtgcgtg aagccaccac cgcctccggc gtggataatg caaccagccc
    40081 ccgactggca gacaccgctg aacgggatta tttcaccctc agagagaggc tgatcactat
    40141 gcaaaaacaa ctggaaggaa cccagaagta tattaatgag cagtgcagat agagctgccc
    40201 atatcgatgg gcaactcatg caattattgt gagcaataca cacgcgcttc cagtggagta
    40261 taaatgccta aagtaataaa accgagaaat ccatttacga atgtttgctg ggtttctgtt
    40321 ttaacaacat tttctgcgcc gccacaaatt ttggctgcat caacagtttt ctcctgtcca
    40381 attcccgaaa cgaagaaatg atgggtgatg gtttcctttg gtgttactgc tgtcggtttg
    40441 tttccaacag taaacgtctg ttgagcacat cctgtaataa gcattgccag agcggcagaa
    40501 aacaacattt ttttcatctt attatcctgc attgttaaaa acggcagaat cctatgtgac
    40561 aacaattaaa cgatagttaa atggattgat gaaaattaaa actatatagg tggatagact
    40621 ggccccctga atctccagac aaccaatatc acttaaataa gtgatagtct taatactagt
    40681 ttttagacta gtcattggag aacagatgat tgatgtctta gggccggaga aacgcagacg
    40741 gcgtaccaca caggaaaaga tcgcaattgt tcagcagagc tttgaaccgg ggatgacggt
    40801 ctccctcgtt gcccggcaac atggtgtagc agccagccag ttatttctct ggcgtaagca
    40861 ataccaggaa ggaagtctta ctgctgtcgc cgccggagaa caggttgttc ctgcctctga
    40921 acttgctgcc gccatgaagc agattaaaga actccagcgc ctgctcggca agaaaacgat
    40981 ggaaaatgaa ctcctcaaag aagccgttga atatggacgg gcaaaaaagt ggatagcgca
    41041 cgcgccctta ttgcccgggg atggggagta agcttagtca gccgttgtct ccgggtgtcg
    41101 cgtgcgcagt tgcacgtcat tctcagacga accgatgact ggatggatgg ccgccgcagt
    41161 cgtcacactg atgatacgga tgtgcttctc cgtatacacc atgttatcgg agagctgccc
    41221 acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact tgatggtatg
    41281 cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct gttgcttgag
    41341 cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc cgtgaaagaa
    41401 agcaatcagc gatggtgctc tgacgggttc gagttctgct gtgataacgg agagagactg
    41461 cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc ggtcactacc
    41521 ggcggcttca acagtgaaac agtacaggac gtcatgctgg gagcggtgga acgccgcttc
    41581 ggcaacgatc ttccgtcgtc tccagtggag tggctgacgg ataatggttc atgctaccgg
    41641 gctaatgaaa cacgccagtt cgcccggatg ttgggacttg aaccgaagaa cacggcggtg
    41701 cggagtccgg agagtaacgg aatagcagag agcttcgtga aaacgataaa gcgtgactac
    41761 atcagtatca tgcccaaacc agacgggtta acggcagcaa agaaccttgc agaggcgttc
    41821 gagcattata acgaatggca tccgcatagt gcgctgggtt atcgctcgcc acgggaatat
    41881 ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtct
//
LOCUS       DACAPB010000013        41887 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.013, whole genome shotgun sequence.
ACCESSION   DACAPB010000013 DACAPB010000000
VERSION     DACAPB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.013"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(82..1335)
                     /locus_tag="IT904_000653"
     CDS             complement(82..1335)
                     /locus_tag="IT904_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP3190483.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            1663..2853
                     /gene="hmpA"
                     /locus_tag="IT904_000654"
     CDS             1663..2853
                     /gene="hmpA"
                     /locus_tag="IT904_000654"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAP3190484.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(2898..3236)
                     /gene="glnB"
                     /locus_tag="IT904_000655"
     CDS             complement(2898..3236)
                     /gene="glnB"
                     /locus_tag="IT904_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAP3190485.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(3297..4631)
                     /gene="glrR"
                     /locus_tag="IT904_000656"
     CDS             complement(3297..4631)
                     /gene="glrR"
                     /locus_tag="IT904_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAP3190486.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(4621..5334)
                     /gene="qseG"
                     /locus_tag="IT904_000657"
     CDS             complement(4621..5334)
                     /gene="qseG"
                     /locus_tag="IT904_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAP3190487.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSNIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(5499..6926)
                     /gene="qseE"
                     /locus_tag="IT904_000658"
     CDS             complement(5499..6926)
                     /gene="qseE"
                     /locus_tag="IT904_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAP3190488.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLHNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(7484..11371)
                     /gene="purL"
                     /locus_tag="IT904_000659"
                     /gene_synonym="purI"
     CDS             complement(7484..11371)
                     /gene="purL"
                     /locus_tag="IT904_000659"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAP3190489.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11629..13185
                     /gene="mltF"
                     /locus_tag="IT904_000660"
     CDS             11629..13185
                     /gene="mltF"
                     /locus_tag="IT904_000660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAP3190490.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(13182..13718)
                     /gene="tadA"
                     /locus_tag="IT904_000661"
     CDS             complement(13182..13718)
                     /gene="tadA"
                     /locus_tag="IT904_000661"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP3190491.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRARDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(13743..14378)
                     /gene="yfhb"
                     /locus_tag="IT904_000662"
     CDS             complement(13743..14378)
                     /gene="yfhb"
                     /locus_tag="IT904_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAP3190492.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            14587..15435
                     /locus_tag="IT904_000663"
     CDS             14587..15435
                     /locus_tag="IT904_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP3190493.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            15491..15751
                     /gene="yfhL"
                     /locus_tag="IT904_000664"
     CDS             15491..15751
                     /gene="yfhL"
                     /locus_tag="IT904_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAP3190494.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHLADKI"
     gene            complement(15945..16025)
                     /gene="shoB"
                     /locus_tag="IT904_000665"
     CDS             complement(15945..16025)
                     /gene="shoB"
                     /locus_tag="IT904_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAP3190495.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(16446..16826)
                     /locus_tag="IT904_000666"
     CDS             complement(16446..16826)
                     /locus_tag="IT904_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP3190496.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(16826..17557)
                     /gene="pdxJ"
                     /locus_tag="IT904_000667"
     CDS             complement(16826..17557)
                     /gene="pdxJ"
                     /locus_tag="IT904_000667"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAP3190497.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(17569..18297)
                     /gene="recO"
                     /locus_tag="IT904_000668"
     CDS             complement(17569..18297)
                     /gene="recO"
                     /locus_tag="IT904_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP3190498.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(18309..19214)
                     /gene="era"
                     /locus_tag="IT904_000669"
     CDS             complement(18309..19214)
                     /gene="era"
                     /locus_tag="IT904_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP3190499.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGTYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(19211..19891)
                     /gene="rnc"
                     /locus_tag="IT904_000670"
     CDS             complement(19211..19891)
                     /gene="rnc"
                     /locus_tag="IT904_000670"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP3190500.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(20163..21137)
                     /gene="lepB"
                     /locus_tag="IT904_000671"
     CDS             complement(20163..21137)
                     /gene="lepB"
                     /locus_tag="IT904_000671"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP3190501.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(21153..22952)
                     /gene="lepA"
                     /locus_tag="IT904_000672"
     CDS             complement(21153..22952)
                     /gene="lepA"
                     /locus_tag="IT904_000672"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP3190502.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(23150..23629)
                     /gene="rseC"
                     /locus_tag="IT904_000673"
     CDS             complement(23150..23629)
                     /gene="rseC"
                     /locus_tag="IT904_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAP3190503.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DAAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(23626..24582)
                     /gene="rseB"
                     /locus_tag="IT904_000674"
     CDS             complement(23626..24582)
                     /gene="rseB"
                     /locus_tag="IT904_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAP3190504.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(24582..25232)
                     /gene="rseA"
                     /locus_tag="IT904_000675"
     CDS             complement(24582..25232)
                     /gene="rseA"
                     /locus_tag="IT904_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAP3190505.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(25265..25840)
                     /gene="rpoE"
                     /locus_tag="IT904_000676"
     CDS             complement(25265..25840)
                     /gene="rpoE"
                     /locus_tag="IT904_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAP3190506.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(25837..25992)
                     /gene="rseD"
                     /locus_tag="IT904_000677"
     CDS             complement(25837..25992)
                     /gene="rseD"
                     /locus_tag="IT904_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAP3190507.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLILCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            26248..27870
                     /gene="nadB"
                     /locus_tag="IT904_000678"
     CDS             26248..27870
                     /gene="nadB"
                     /locus_tag="IT904_000678"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAP3190508.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(27855..28592)
                     /gene="trmN"
                     /locus_tag="IT904_000679"
     CDS             complement(27855..28592)
                     /gene="trmN"
                     /locus_tag="IT904_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAP3190509.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            28724..30058
                     /gene="srmB"
                     /locus_tag="IT904_000680"
     CDS             28724..30058
                     /gene="srmB"
                     /locus_tag="IT904_000680"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAP3190510.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(30267..31148)
                     /locus_tag="IT904_000681"
     CDS             complement(30267..31148)
                     /locus_tag="IT904_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3190511.1"
                     /translation="MDLCRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRSTVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            31251..31838
                     /gene="eamB"
                     /locus_tag="IT904_000682"
     CDS             31251..31838
                     /gene="eamB"
                     /locus_tag="IT904_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAP3190512.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(31894..32277)
                     /gene="grcA"
                     /locus_tag="IT904_000683"
     CDS             complement(31894..32277)
                     /gene="grcA"
                     /locus_tag="IT904_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAP3190513.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            32582..33271
                     /gene="ung"
                     /locus_tag="IT904_000684"
     CDS             32582..33271
                     /gene="ung"
                     /locus_tag="IT904_000684"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP3190514.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(33319..34356)
                     /locus_tag="IT904_000685"
     CDS             complement(33319..34356)
                     /locus_tag="IT904_000685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP3190515.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            34563..34982
                     /gene="trxC"
                     /locus_tag="IT904_000686"
     CDS             34563..34982
                     /gene="trxC"
                     /locus_tag="IT904_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAP3190516.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            35051..35749
                     /locus_tag="IT904_000687"
     CDS             35051..35749
                     /locus_tag="IT904_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAP3190517.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     YYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            35781..38441
                     /gene="pat"
                     /locus_tag="IT904_000688"
     CDS             35781..38441
                     /gene="pat"
                     /locus_tag="IT904_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAP3190518.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALMEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            38555..39910
                     /gene="pssA"
                     /locus_tag="IT904_000689"
     CDS             38555..39910
                     /gene="pssA"
                     /locus_tag="IT904_000689"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAP3190519.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            39935..40279
                     /locus_tag="IT904_000690"
     CDS             39935..40279
                     /locus_tag="IT904_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAP3190520.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDHSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(40276..41574)
                     /gene="kgtP"
                     /locus_tag="IT904_000691"
     CDS             complement(40276..41574)
                     /gene="kgtP"
                     /locus_tag="IT904_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAP3190521.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        10099 a        11121 c        10550 g        10117 t
ORIGIN      
        1 aagattttgt aggcctgaga aggtgttcat gccgcatcca acatgaacaa cgagcacatt
       61 gtcagcaaat caccgtttcg cttatgcgta aaccgggtaa cgtgcgcaga tgtcgagaac
      121 tttacctttg atgcgctcga taacggcttc atcattgatg ctgtccagca cgtcacacat
      181 ccagccagcc agttctttcg cttcggcttc tttaaagccg cgacgggtaa tcgccggagt
      241 acctacacga ataccggagg tcacaaacgg gctcttcgga tcgttcggta cgctgttttt
      301 gttgacggtg atgttagcac ggcccagagc ggcgtctgct tctttaccgg tcaggttttt
      361 atcaaccaga tcaaccagga acaggtggtt atcagtgccg ccggaaacca ctttgtagcc
      421 gcgttccagg aacacttcta ccatcgcttt agcgttttta gcgacctgct gctggtaagt
      481 tttgaactca ggctccatcg cttctttcag agcaaccgct ttaccggcga ttacgtgcat
      541 caacggaccg ccctgaccac cagggaaaac ggcagagttc agttttttgt acagctcttc
      601 gctaccacct ttcgccagga tcaggccgcc gcgcggaccc gccagggttt tgtgagtggt
      661 ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag acgccagcag caaccaggcc
      721 cgcaacgtgc gccatatcaa cgaacaggta agcaccgatg ctgtcagcga tttcacgcat
      781 tttcgcccag tccaccacgc cggaatatgc agagaaacca ccgataatca ttttcggctt
      841 gtgttctttg gcttgttttt ccagatcggc gtagtcgata tgaccggtag catcgatacc
      901 gtaaggaacg atgttgtaca gtttaccgga gaagttaacc ggagaaccgt gagtcaggtg
      961 accgccatgc gccaggttca tacccagaac ggtatcacct ggttccagca gcgcggtgta
     1021 gaccgcaaag ttagcctggg agccggagtg cggctgaacg ttagcgtagt cagcgccgaa
     1081 cagttctttc gcacgatcga tcgccagttg ttcaacgata tcaacatact cgcaaccgcc
     1141 gtagtagcgt ttgcccggat aaccttcagc atatttgttg gtcagctgag aaccctgcgc
     1201 ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc agttcgatgt gctcttcctg
     1261 acgtactttt tcctgctcca tagcctgcca cagttcggca tcataatcgg caatgttcat
     1321 ttcacgcttt aacatccgca tctcctgact cagctaacaa taaaattttt ggcctttata
     1381 ggcggtcctg ttggacaacg gcgaacagta taaccgaatc attgtgcgat aacaggtctt
     1441 gacaaaggaa tttacgcaaa cgattacctt caggctacgc aaggctttgg cgaataaaga
     1501 gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg caaatttttc acttcatcac
     1561 attctttctg aaaaacacca aagaaccatt tacattgcag ggctattttt tataagatgc
     1621 atttgagata catcaattaa gatgcaaaaa aaggaagacc atatgcttga cgctcaaacc
     1681 atcgctacag taaaagccac catcccttta ctggtggaaa cagggccaaa gttaaccgcc
     1741 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
     1801 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
     1861 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
     1921 agtttccaga tcaaaccgga acagtacaac atcgtcggcg aacacctgtt ggcgacgctg
     1981 gacgaaatgt tcagcccggg ccaggaagtg ctggacgcgt ggggtaaagc ctatggcgta
     2041 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
     2101 ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt
     2161 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
     2221 caatatctcg gcgtctggct gaagccggaa ggtttcccac atcaggaaat tcgtcagtac
     2281 tctttgactc gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg cgaagagggt
     2341 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
     2401 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
     2461 gccggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
     2521 acagcacaag tgaactggtt ccatgcggca gaaaatggcg atgttcacgc ctttgccgat
     2581 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
     2641 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
     2701 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
     2761 ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
     2821 tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg
     2881 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
     2941 acccgtgcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
     3001 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
     3061 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
     3121 tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt
     3181 gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta
     3241 ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
     3301 tccttgaaat cgtttgcatc cagctcgtgt cgagaaagca gtttataaaa ttctgtccgg
     3361 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtaatttg cagcagctta
     3421 cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa aggttggcag cgccgtgttt
     3481 tcaccctcca gcgcctgctc tactaatgca tcgctaataa ccggagatga ggtcagcgcc
     3541 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct agcggtcatc
     3601 aggcgtttca tcgcatcagt ggagaacgcg cggacaaacg gtttatgtcg ctctgccgcc
     3661 tggcgcaaca ggtgatttgc cagaagcgga atgtcttctg tgcgctctgc cagagccgga
     3721 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
     3781 atcgcttttg gcagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
     3841 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt
     3901 aacggcgcag gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg
     3961 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
     4021 tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg
     4081 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga
     4141 ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
     4201 agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg
     4261 gattgctcca gcgcatcgtc aattgcctga tatagcgcgt ctttgtcgac aggcttggtg
     4321 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca
     4381 gtaagaataa ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata
     4441 ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt
     4501 acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg
     4561 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
     4621 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg
     4681 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt
     4741 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
     4801 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc
     4861 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
     4921 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
     4981 cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
     5041 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
     5101 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gtgcccagat
     5161 gttgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
     5221 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg
     5281 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
     5341 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg
     5401 cggagaaacc ggagcgtaca tatcgtacgt gaagatttcg agcactgccc ggggccgaac
     5461 tgacaaataa aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac
     5521 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
     5581 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc tttcaccgcc
     5641 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
     5701 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
     5761 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
     5821 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
     5881 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc
     5941 ggtgctaact caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca
     6001 agcagttgtt cgatcagttt ttgcaaattg cggctgctgc tatcaagaat gctcaccacc
     6061 tctttttgct ctggcgtaag cggcccgaca acctggtcag ccagtaattc agtgccctcg
     6121 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
     6181 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
     6241 aactcgctcg gtccactgaa cgagacgcta ttgcccagag aacgcccttc ccccagacgg
     6301 ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagcagtacc
     6361 attaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
     6421 gcgatttcac gctgaagctg ctgcccacga gagaacacca ctgtgcgcgt ggcctgtacc
     6481 atttcggtat tggcactggc aaaggcttca agacgtgcgg cggcggcagc atcgggaccg
     6541 ctgttgttac actgaagttg agccagattg tgcaaatcct gacgtaatgc ctggtagagt
     6601 ttatcgtccg gcagcacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
     6661 ctttgataaa ccttcgccag ggttgggtcg tccagcacgc aatactgacg gtaactacgc
     6721 tccatctcca gcgccgcgtt ggtcatcgct tcactgcgcc gggcatcaat aagcgtagtg
     6781 cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc
     6841 agcaccaaca ggggcagcag aatcagcaaa aatgccagca ttaccagttg tcgtaatgag
     6901 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
     6961 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
     7021 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
     7081 gttggacggc aggcaccttg ttgtgcgtca ttcgtatttt atgtagcacg tcccgaaggg
     7141 gctgacataa gtcggtgaat gagccactgg ttactattat gcagtaactg tgccaataaa
     7201 gaaaatagtt tggtaacgta ctgattatac gttgctttga ggggtttatg tctcctccgc
     7261 tgtttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt
     7321 agataatatt aaaatcaatg agttaagtgt ctccagaaag cgacacggca aaccacccat
     7381 tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt
     7441 gcttaatgcc gcatccgggc tgcaaaacca atgggctgac gacttacccc aactgcttac
     7501 gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg
     7561 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac
     7621 tttcagtcgt gactgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt
     7681 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
     7741 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
     7801 gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca
     7861 ggctgaaacg cgcttcaaag cgatcggagg tattgcgcac aaaacgtggc cacaactcac
     7921 tacctgggat cagttcacgc agattagaca tcatctggca accgttacat acccccagcg
     7981 ccagcgtttg cggacggtgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
     8041 ggattgactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaaccaccgc
     8101 acgcgaccag ggcgtggaaa tcttccaggc ccgtgcgtcc ggtcagcagg tcactcatat
     8161 gcacgtcgat agcatcaaag cctgcacggt ggaaagctgc cgccatttca acatgcgagt
     8221 tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg
     8281 ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt
     8341 tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
     8401 gccaggtagt ttctgcccac cagacacgca acgtggtgcg gctttcgctg aatacagtct
     8461 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgccctaca taatggacac
     8521 aatcagcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
     8581 cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
     8641 tggcgatatc cgcgtcaatg ccacaatgac cagcaaaggc catttccgcc agcgttacca
     8701 gcaggccgcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct
     8761 gaatcgcgtc atagaagcct ttcagttgcg cgacatcgcg tacatctgcc ggtttgtcgc
     8821 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
     8881 aatcaatcag cagcagtgcg ttatcttcgg tagaaagctg cggcgtgatg gtgtgacgta
     8941 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
     9001 cttcgttacc ttcctgccag cgggttttca tcgacatgga gtctttaccc accgggatcg
     9061 tcaggcccag cgccggacaa agctcttcgc ccacggcttt aacggcttca tacaggcccg
     9121 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagtttg atgcgtttga
     9181 tatcgccaat ttgtgttgcg gcgatgttgg ttaacgcttc accgaccgcc agacgggcag
     9241 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
     9301 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
     9361 ccaccatctg atcgcgcgct accatgccgg ttacgctgcg gtcgccaatg gtcaccagga
     9421 aggttttttc cgccacagtc ggcagatgca gcacacgttt caccgcgtct gcaatggtga
     9481 tcccttcacg ggccagcgcg tcgcctttcg ctttcagcgt ttgtacatcg cgggtcatct
     9541 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
     9601 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
     9661 gttcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
     9721 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
     9781 gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg
     9841 cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa
     9901 gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct
     9961 gtacggaagc aaagtcgagg tcggcatcag actgacctga cgccatggaa gacgccgctc
    10021 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt
    10081 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    10141 tcggtttgtg ataaccgcgc agctcttcgc cgttgtggct gttcactttt tcttcataag
    10201 tacggaagta gccattcagt gccggacgac caaattcgtt gttaaacgcc gcgccgccca
    10261 gcgggccttc ggtcatgatg tccagcgcgg tgacaatgcg ctcaggctta ccgaaatctt
    10321 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga aacggagaaa ccaaccaaac
    10381 ccgctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    10441 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    10501 tcagaatatg cgccggttcc tgatggaaat cgtagcggcc cgtttcgtgg tcagcaaagt
    10561 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    10621 aatctggcgt ggtttcgaag gtgtttttga tcatcttaaa cagcgatttc ggctgttgct
    10681 caccgtcgat aacccaatcg gcgttaaaaa tcttgtggcg acagtgctcg gagttcgcct
    10741 gggcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    10801 gcagatagtc aatttcatct tccgccagtg ccaggccaag acgcaggtta gcgtcgatta
    10861 gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg
    10921 caaacaactg ctctgcatca tctaaagcaa aaaagaccgt ttccatcatg cggtcgtgca
    10981 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    11041 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg
    11101 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    11161 gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct
    11221 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    11281 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    11341 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    11401 acccaagggg gggaaacggg cgtcattata aagaatctga tgcgctgacg aaaccgtttg
    11461 cgtggaaata aaatcaccat cgtgaattag caacgcgtgc cgccaatgga tgtaataagt
    11521 tgccatctgg cgcaggttta cgcaaaatgc cgctcattta tgagtaaacc tttcactatt
    11581 attacgtttt ttcaagctgg gacgcgcacg acacagagaa ttaactaatt gaaaaaatta
    11641 aagattaatt atctgttcat cggcattctg gcactgctgc tcgcggtcgc tctctggcca
    11701 tccattccct ggtttggtaa agccgacaac cgtatcgccg ccattcaagc gcggggagag
    11761 ttgcgtgtga gcaccattca tactcccctg acttataacg aaatcaacgg gaaacctttt
    11821 ggcctggatt acgaactggc gaaacagttt gccgattacc tcggcgtaaa actgaaagtg
    11881 accgtgcggc agaatatcag ccagctgttt gacgaccttg ataatggtaa cgccgacctg
    11941 ctggcggcag gacttgtcta taacagtgag cgggtaaaaa attatcagcc tggccctacc
    12001 tattattccg tgtcacaaca actggtttat aaagtgggtc agtatcgccc acgtacgctg
    12061 ggcaacctga cggcggagca actcaccgtt gcaccgggtc atgtggtggt taacgatctc
    12121 cagaccctga aagaaacaaa attcccggaa ttaagctgga aggtagacga caaaaaaggc
    12181 tctgcggaat taatggaaga tgtcatcgaa ggaaaactcg attacaccat tgctgattct
    12241 gtcgccatca gcctgtttca gcgcgttcac ccggagctcg ccgtagcgct cgatatcacc
    12301 gatgaacaac cggtgacttg gtttagcccg ttagatggcg ataataccct ttccgccgcc
    12361 ctgctcgact tcttcaacga aatgaatgaa gacggtacgc tggcacgcat tgaagagaaa
    12421 tacctggggc atggcgatga ttttgattac gtcgatacgc gcacattttt acgcgccgtc
    12481 gatgcggtac tgccgcagtt aaagcccctg tttgagaaat acgccgaaga aattgactgg
    12541 cgtttgctgg ccgctattgc ttatcaggaa tcgcactggg atgcacaggc cacttcaccg
    12601 acgggtgtgc gcggcatgat gatgttaacc aaaaataccg cgcaaagcct cggcattacg
    12661 gatcgtaccg atgccgaaca gagcatcagc ggtggcgtgc gttatttgca ggatatgatg
    12721 agtaaagtgc cggaaagtgt gccggagaac gagcggatct ggtttgccct cgctgcgtac
    12781 aatatgggct atgcgcatat gctggatgcc cgcgccctga cggcaaaaac caaagggaat
    12841 cctgacagtt gggctgacgt aaaacagcgt ctgcctttac ttagccagaa accctattac
    12901 agcaagctga cttacggcta cgctcgtgga catgaagcct acgcttatgt cgaaaatatt
    12961 cgtaagtatc agattagcct ggtgggttat ctgcaagaga aagagaagca ggctacagaa
    13021 gcggcgatgc aactggcgca ggattatccg gcggtatcgc ctacggagtt gggcaaagag
    13081 aaatttcctt ttctctcgtt tctttcccag tcgtcatcaa actatttgac ccattctccc
    13141 tctctgctgt tttccaggaa agggagtgaa gagaaacaaa attaatccgt cgaggattgc
    13201 gcttttttct gcgctttaat ttcctggcgg cgcatgcgaa agaagtcact gagcaacgcc
    13261 gcgcactcat ccgccagtat tccttccgta atttccactc ggtgattcat acccggatga
    13321 tgcagcacat ccattaaaga tcccgcagcg ccagttttcg cgtcacgcgc accaaagacc
    13381 acgcgaccaa tgcgactgtg gatcatcgct ccggcacaca ttacacatgg ttcaagcgtg
    13441 acatacaatg tggcgtcgat cagacgataa ttttgcatca ccagaccacc ctgccgcagg
    13501 gccatgattt ctgcatgtgc ggtgggatca tggcgaccaa tcgggcggtt ccagccttcg
    13561 ccgattaccc gattgttatg cactaatacc gcgccgaccg gcacttcccg ctcatccctg
    13621 gcacgtttcg ccagcgtcag cgcgtgacgc atccagtatt cgtggctaaa ttcgacttca
    13681 gacaaaaaga aaactccggt tataaaagcg cggcgcatta tacacggacg ctatgcttta
    13741 ctctattcca gttgctggag ttcaccgcgc ggggttactc gccaacgatg ctgacagaaa
    13801 taaagcagcg gattgtcctg attactgtcg ctatagccac tgtacagccg cagcggagtg
    13861 ccgattttgc gctccagttg tgcgaccttt tcatgtccca gacaacgcat cgtcaatacc
    13921 caaccaccat agccacgctg aatttggctg gcgataagat taacccgcgg cagccagggc
    13981 gtatcgaaat aaaccgcttc aaccagcggc tgcggagagc cggtaatcaa ccagatatca
    14041 gcatcggaac ttaacaggta ggtggttaat cgctcctgaa ccagcggaaa ggcggtaaca
    14101 ttgtcgcgaa accagcgcac gaaatcggcc tgcaacgtct gtaaacgtgc ttcgctgtga
    14161 ccaaaagtgc acccccacag aagcagactc atcggccagc gtgccgcacg accttttatc
    14221 aataacgcaa tggctataat cggtaacaac ggcaggacaa gtaacgcatt cagcggttgg
    14281 cgacgtagta aatagcgcag aaaactgccg aacatatcct gctgatgcaa tgttccatct
    14341 aagtcaaaaa acaccacacg acgctcgtga gttgccaaac catactcctc tggactgaaa
    14401 catcctgatt aattttcgct actacatagc ctaacagata gatcatcact tttccggcaa
    14461 caatcgaccg catcatggct aactggaatt tttaattcat ggcaattagc ggcaatggaa
    14521 tataaaattc actcgcgtgt gtctcatatt gcctgttgtc gccactattt catcgcgagg
    14581 aaagatatga acggcttact tcgtatccgt cagcgttacc aggggcttgc ccagagtgat
    14641 aaaaaactgg cggattatct gctgctacaa cctgatacgg cgcgccattt aagctctcag
    14701 caactggcca acgaagccgg agtcagtcag tccagcgtcg tgaagttcgc gcaaaaactc
    14761 ggctataaag gttttccggc gcttaagttg gcgttgagtg aagcgctggc aagccagccg
    14821 gaatcaccct ccgtgcccat tcataaccaa atccgcggtg atgatccgtt acggctggtc
    14881 ggcgaaaaac tgattaaaga aaatactgcc gctatgtacg caacgctaaa cgttaatagt
    14941 gaagagaaac tgcatgaatg cgtaacaatg ttgcgctctg cgcggcggat aattctgacc
    15001 ggtattggcg cttcgggtct ggtggcgcaa aactttgcct ggaagctgat gaagattggc
    15061 ttcaatgctg ccgcagtgcg cgatatgcat gcgctactcg caacagtaca ggcgtcgtcc
    15121 cctgacgatc tgttattagc catttcctac accggtgtac gacgcgagtt aaacctggcg
    15181 gcagatgaga tgctgcgagt gggcggaaaa gtgctggcga ttaccggctt tactccgaat
    15241 gccctgcaac agcgtgcttc tcattgcctg tataccattg ccgaagaaca agcgacaaac
    15301 agtgcttcaa tctctgcttg tcacgctcag ggaatgttaa cggatttgct gttcattgcg
    15361 ctgattcagc aggatctgga actggcacca gaacgtattc gtcatagtga agcgctggtg
    15421 aaaaaactgg tctgagtaaa gaatgcgcgt ataatgcccg cccggtttgt gttgttttga
    15481 gagtttcctt atggcgttgt tgatcactaa aaaatgcatc aattgtgata tgtgtgaacc
    15541 cgaatgcccg aatgaggcga tttcaatggg agatcatatc tacgagatta acagcgataa
    15601 gtgtaccgaa tgcgtagggc actacgagac accaacctgc cagaaggtgt gcccgatccc
    15661 caatactatt gtgaaagatc cggcgcatgt cgagacagaa gaacagttgt gggataaatt
    15721 tgtgctgatg cacctcgcgg ataaaattta atattctact ctggaagtag agtattaatt
    15781 atattactgg gaagccttaa cgccattata tttatttaat tgatgacatt agcataatca
    15841 ttcactaagt taatttatat agtatctgcc cagacactta tttatagtta ttaaaggtgc
    15901 gtccgactgg ttcaccggac gcaccttaag tacgtttcct tgtgttataa gaacagaagg
    15961 atcagctgta aaacagcaat gatgattttg atgacccgtt taatcaggta tcggcaatca
    16021 gtcattcgtt tttccttaaa caaggaatgc agccattcag attagccctt acatctcccc
    16081 aaaactgaac gtgcgagtta ttgagggtgc atgctgcact ccacaccaga gctttgacga
    16141 caccactcgt ttcaatgggg gaattctgtg gcatggtgta aagcacagca aaatcttcaa
    16201 taacgaagcc aattttaatg tacttacgaa ttggcggtca cgttaatctt tccatcaata
    16261 ttgctttctt cgtaaaggct cgagttttta tgctaaagat tgcaagttgc ttgtaaaaga
    16321 taagtacact gatccataat cgctgttgtt gagggtgcat gctgcacaaa attaaagtta
    16381 aaaagtaaaa cccccgttcc ttaccagttc gggggtttta ctttttaaag agaacggtat
    16441 tatttttaac tttcaataat taccgtggca caagcatagt gccgctcatc tgccagcgtt
    16501 acatgcatat ttgcaacgcc cagcttttcc gccagtttta atgcctcgcc ccatagccgt
    16561 agccgtggtt tgccgagctc atcattgaat acttcaaatt gattaaacgc cagaccattg
    16621 cggatcccgg tgccaaacgc ttttgctgcg gcttctttca cagcaaaacg cttcgccaga
    16681 aaacgcaccg gctggtggtg cgttttccag atagcccatt cgttatcgct taatacgcgg
    16741 cgtgccaggc gatcaccgga tcgggcgatc accgcttcga tgcgagcgat ctccacaata
    16801 tccgtgccta aacctaatat tgccattagc cacgcgcttc cagcatcaga cgcttcattt
    16861 ctgccaccgc atctttcagt ccggtcatca ctgcacgacc aataatggca tgaccgatat
    16921 tcagttcatg catctcaggg atggcggcaa tggctttcac gttgtgatag gtcagaccgt
    16981 gtccggcgtt aactttcaga ccgaggcttg cggcaaaggt cgcggctttg gcgatacgcg
    17041 ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc atagcaaccg gtgtggatct
    17101 cgataaacgg tgcgccaacc tctgccgcag ctttgatctg ctcttcatcg gcgtcaataa
    17161 acagagaaac ctgaatcccg gcatctgcca gacgtttgca ggcatcgcgc attttgtcac
    17221 gctgccctgc gacatccagg ccgccttcgg ttgttacttc ctgacgcttt tccggtacca
    17281 ggcagcaaaa atgtggcttc gtctcaacgg cgatcgccag catctcttcg gtcaccgcca
    17341 tctccagatt catgcgggta tccagcgtct gacgcaggat gcgcacgtcg cggtcagtaa
    17401 tgtgacggcg atcttcacgt aaatgcacgg taatgccgtc cgctcccgcc tgctcggcaa
    17461 taaacgcggc ctgcaccgga tccgggtaag cggtaccgcg cgcgttgcgc agcgtagcga
    17521 tatggtcaat gttgacgcct aacagtaatt cagccatgac aatcctcatc attcataatg
    17581 tgttttcacc gttcgcttag gcataaattg ccggaacagt tccctgctct ttaaaggttt
    17641 accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt ttcgcggcgc gcagtgtgtc
    17701 tgcgtcagga aattcccgtg cgtttaacgc ttttaactgc cttccggtga acgttttatt
    17761 gtcgataacg acgcttgcga taaacccttt ttcttcgcga taacgatacg tcatggtgtc
    17821 atctaccggc tcgccgctac ccgcacaatg ggtaaaattg acgccataac ccagatgccc
    17881 gagcagtgcc agttcaaagc ggcgcagcgc gggttctggc gtaccagtga cccctgcaag
    17941 agactgaatg cagtgcaagt aatcgaaaaa aagttcagag aagcgcgtct cgtattccag
    18001 tacgcgggag agaagttcgt tgatgtacag accgctgtaa agcgtgatac cgcttaatgg
    18061 cagcgccagc gagacggctt cagcactgcg cagcgttttg acttcgccac gcccgccaaa
    18121 acgtagcaag agaggggtga aaggctgtaa tgcacctttc agggtagagc gtttagagcg
    18181 tgcgcctttg gcaaccagac gcacgcgccc cgattcctcc gtgaagacgt ccagcatcag
    18241 gctggtttcg ctccacgggc gactatgcag gacaaatgcg cgctgccagc cttccatcgg
    18301 agttactctt aaagatcgtc aacgtaaccg agactgcgca gtgcgcgttc gtcgtcggcc
    18361 caaccggatt tcacttttac ccacagttca aggtgaacag gcgcttcgaa catttcctgc
    18421 atgtctttac gcgcttcaat cccgatggtt ttgatcttgg cccctttgtt gccaatgacc
    18481 atcttcttct gcccttcacg ctcaacgaga atcaaaccgt tgatgtcata accaccgcgt
    18541 tcgttagaga cgaaacgttc gatctccacg gtcacggagt acggcagttc agcgccgagg
    18601 aaacgcatca gtttttcgcg gatgatttca gacgccataa aacgctgtga gcgatcggtg
    18661 atgtaatctt ccgggaagtg atgagtcgct tcaggtagat gcttacgcac gattgccgca
    18721 atagtgtcaa cattcagccc ggtttcggca gagattggca cgatatcgag gaagttcatc
    18781 tggcttgcca ggaactgcag gtgcggcagc agatcggctt tctcctgcac gttgtccact
    18841 ttgttcaccg cgaggattac cggcgctttg ccttcgcgca gtttgttgag caccatttcg
    18901 tcgtccggcg tccagcgggt gccttcaacg acaaaaatca ccagctcaac atcgccaata
    18961 gagctgctcg ccgctttgtt catcaggcgg ttaatggcgc gtttttcttc catatgcagg
    19021 cccggtgtat cgacgtagat cgcctgatac gtgccttcag tatggatccc cacaatgcgg
    19081 tgacgagttg tctgcgcctt gcgggaagtg atggagattt tctgccccag cagtttgttc
    19141 aacaatgtgg atttgccaac gttcggacgt ccgacgatgg caataaatcc gcagtaactt
    19201 ttatcgatgc tcattccagc tccagttttt tcaacgcctg ttcggcggca gcctgctcag
    19261 ccttacgacg gcttgaacct gtgccaacca ccggttcact caggccgctg acctggcagt
    19321 ggatagtaaa ttcctgatcg tgcgcttcgc cacgtacctg gactaccaga taagtcggca
    19381 gcggcagatg gcgaccctgc aaatattctt gcaagcgcgt tttcggatct ttttgtttat
    19441 cgcctgggct aatttcgtcc aaacgagttt gataccagtt gaggattaat ttctcgacgg
    19501 tttgaatatc actgtcgagg aatacgccac caattaatgc ttcgacggtg tcggcgagaa
    19561 ttgactcacg acgaaatcca ccgcttttaa gttcacctgg ccctaaacgt aagcactcgc
    19621 ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc acggaccagc gtggcgcgca
    19681 tccggctcat atcgccttca tccacacgag ggaaacggtg ataaagcgca ttggcgataa
    19741 cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc gttatgttta ctgctggcac
    19801 tacgatgagt taatgcctgc tgcaacagtt cctgatgatt aaaagtgtag cccagcttcc
    19861 gttgaagccg attaattacg atggggttca tgcgatacca ataaataaat gcgtcaacaa
    19921 ttcagcacac gaaacagacc tgatatacat ggttctgcta actgcttcgc tgcagtttca
    19981 gtttaccggt atatggggac caacgctgtt tcgtgtgccg tggcaacctg gaggtgccaa
    20041 ccttaaactt cgggggaata ttctatacac aacgacgggg gatgtcgtta gccacgggag
    20101 atttatctca taaataattc acgttgtcgc cataacggcg acaacgtgaa cgaagatggc
    20161 tattaatgga tgccgccaat gcgacttaag cgcagaccag tcggccattc gccttcttgc
    20221 ttatcgaagc tcatccagat agccgttgcc cgaccgacca gattcgcttc cggcacaaag
    20281 ccccagtaac ggctgtccgc gctgttgtcg cggttgtcgc ccatcatgaa gtattgtccc
    20341 ggaggaacaa tccaggttgc cagttgttgc cctggctgct ggtaatacat ccccacctga
    20401 tcctgcgcaa tcggcactgt cagaatgcgg tgcgtcacat cacccagtgt ctctttacgc
    20461 tcggaaagac gaattccatt ttctttggtt tcgtttttcg gcacttcaaa gaatccgctg
    20521 gtcgcttccc caccattacg gcgtgagaag gtctgaacga aatcgctcgg ttccacgttt
    20581 gagtaggtga ccggcagcgc gttttcacac gcctggccgg aactgcatcc cggttgaatc
    20641 gtcagctctt ttgagaccgg atcgtaagtg actttatcgc ccggtaaacc caccgcgcgc
    20701 ttgatgtaat caagctttgg atcttccgga tatttaaaga ccacgatatc gccgcgtttc
    20761 ggatgaccgg tttcgatcag cgttttctgg tagataggat ctttaatgcc ataagcaaac
    20821 ttctctacca gaataaaatc accaattaac agagtcggca tcatcgaacc tgacgggatc
    20881 tggaacggtt cataaataaa cgaacgcaca atcaatacga tagccagtac cggaaaaaca
    20941 gaagcaccgg tttccagcca gccaggcttc ggcgcaacct ttttcaacgt tgctttatcc
    21001 agtgagtccc cggcagccgc ctgcgccgct gcctgacgtt cccgccgttt aggtgcgaaa
    21061 aagaatttat ccacgcacca taaaatgccc gtcaccagtg tggcaatcac cagaatcagg
    21121 gcaaacatat tcgccatgcc aactcctaag ggttatttgt tgtctttgcc gacgtgcaga
    21181 atggcgagga acgcttcctg cggcagctcg acgttaccga tctgcttcat gcgtttctta
    21241 ccttctttct gcttctgcag cagctttttc ttacggctga tatcgccgcc ataacattta
    21301 gccagtacgt ttttacgcag ctgtttcacg gtggatcgcg caatgatgtg cgtaccaatc
    21361 gctgcctgaa tggcgatatc aaactgctgg cgtgggatca gatctttcat cttctccacc
    21421 aactcgcgac cgcggttttg cgaattatca cggtgggtga tcaacgccag cgcatcaaca
    21481 cgttcaccgt tgattaatac gtctacacgt accatgtcgg acgcctggaa gcgcttgaag
    21541 ttgtaatcca gagacgcata accacgcgag gtagatttca ggcgatcgaa gaagtcgagc
    21601 accacttccg ccatcgggat ctcgtacgtc agcgccacct gattaccgtg gtaaaccata
    21661 ttggtctgca cgccgcgttt ttctacgcac aacgtaataa cgttgccgag atatgcctgc
    21721 ggcagcagca tgtgacactc tgcaatcggc tcgcgcagtt cgtagatgtt atttaccgca
    21781 ggcagcttgg atgggctgtc gacgtagata acttctcttg acgtggtttc aacttcatac
    21841 actacggtcg gcgcagtggt gatcagatcc agatcgtatt cacgttccag acgttcctgg
    21901 atgatctcca tgtgcagcag gccgaggaag ccgcagcgga aaccaaagcc cagcgcgctg
    21961 gagctttccg gctcatagaa cagtgaggca tcgttcaggc tgagtttacc cagcgcgtca
    22021 cggaaggctt catagtcgtc ggaacttacc gggaacagac cggcgtatac ctgcggtttg
    22081 actttcttaa agccaggcag cgccttttct gccggattac gcgccagcgt taaggtatcg
    22141 ccgactggag cgccgtggat atctttaatc gcacatacga gccagcctac ttcgccacat
    22201 ttcagttcag tgcggtcaac ctgtttcggc gtgaagatgc ccagacggtc ggcgttatag
    22261 gtctgcccgg tactcatgac tttcactttg tcgcccttac gcagggtgcc gtttttaata
    22321 cggataagtg aaacaacgcc caggtagttg tcgaaccatg agtcgataat tagtgcctgc
    22381 aacgggcctt ccggatcgcc ttccggcggc ggaatgtcgc gcaccagacg ttcgagaacg
    22441 tcctgcacgc caacaccggt tttcgctgaa cagcgcaccg cgtcagtggc gtcgatgccg
    22501 acgatatctt caatttcttc cgccacgcgt tcaggatcgg ctgccggcag gtcaatcttg
    22561 ttcagtaccg gcaccacttc gagatccatt tccatggcgg tgtaacagtt tgccagggtt
    22621 tgcgcttcca cgccctgccc ggcgtcgacc accagcaatg caccttcaca ggcagccagc
    22681 gaacgggaaa cttcatagga gaagtctacg tggcccgggg tgtcgataaa gttaagctga
    22741 taggtttcgc cgtcagacgc tttgtagtcc agcgtcacgc tttgcgcttt gatggtaatg
    22801 ccacgctcac gctcaagatc catggaatcg agaacctgcg cctccatttc acggtcagac
    22861 aggccaccgc agatctggat aatacggtca gacagcgtcg atttaccgtg gtcaatgtga
    22921 gctatgatcg aaaagttacg tatattcttc ataaagtatg attattgtgc cttaatgccc
    22981 ggttaaccag gcttttagaa gtcgctgttc tgagcttaac gtctgtatta atagaaacgc
    23041 cgcattctac actacaacat tgaggcgagg aaatgttcat accgtatgga ttgtggtatc
    23101 tggaaacgtc ctcgcatttg ttatgcaaaa tgcaacaaag ccagtgaaat cactggctcg
    23161 cgtcttccga agatgtttca aatcgcacaa ggccaggcgg caatgccacg cttaagatga
    23221 tcggttgcca ttctgcccgg gcagcaaact tacgcgagta gccacgcgca atcaggaacc
    23281 cgccaatacc gccgaggacc gcaccacata atgctgcggc gtcagaagca aagagtagct
    23341 gaaatagcga agcgatgagg aataatccca ccagcggcga catataaacc agtaatgcgg
    23401 agctaagcag gctgccttcg gcgatcccca attccacttt ttgccccggc actaacggct
    23461 catcacaggg tacgacaatg gtatgcgtgg tttgcgggcc aagtttattc aacacgcggc
    23521 taccgcaacc ggcgcgtgaa gcgcagctgc tgcatgaggc tttaacatca caactgacca
    23581 gcgcctgccc gttttgccag gagacgacgg tagcccactc tttgatcatt gcgctgcccc
    23641 gaacttaata ttctcggcaa tgcgtttcgc cgtttgcggc ggcagttcac cgacaatggt
    23701 gatttcggcg ttatcacgta cgcttgtact gacggttctg cgtccggtgc gcaacatctg
    23761 atcggtgctc gatggcgtag cgcggttaac gtttaccgag aagctgaata atccgtcgga
    23821 atagagacgt gattcgatag gcatgttgtc catcgtcggt agcggacgtc gactactgga
    23881 aacttcgcta aaaccctgtg gcaaccaggt tggcgtccag ctgaatttag ctttttcacc
    23941 tacaggaaca gaaagcaacg gcggcaaatt tgccttcgcc aacgtctgca tactgctgct
    24001 gatatcctga ttgacgttaa aagcaatcac gcgaaattgt tccagcgttt caccatcgcg
    24061 atcaaggaga tcaaccctca tcggtaattt cgattcagtg tccatccaca cgatgtagct
    24121 gtagcgtgta ccatctcggg caaccacgcg aatgacttcg caaagacgat cagcaatacg
    24181 cgtgcgcccg acggagatga agtcgtagta aggagaaagg cgtttgaaat cggtatagat
    24241 gagcgatggc agagaatcaa caatgtaatc gccattaagc gtgaacggtt caagtcccgg
    24301 ttcaaaatag ctgatttcat tgccgcgctg taccacttcc cggcgcgggc catccatttg
    24361 caacaattgt gcaagaggac ggttatcgag gcgtgcatgt cgataacgca gagactcaac
    24421 accctgttta ttgatgctga tgaatgacag ctcgtaattc agtgactgac tggccaggtt
    24481 catctgctgt agtaacgccc cggacgcggg agtggccgag gcgttagcag agaataacag
    24541 gctacctgtc actaatgaca tggcaaacca aagttgcttc attactgcga ttgcgttcct
    24601 aaagtttgaa ttcctggcac ctgtacagcg gcttgctggg tttgcgcctg ctcaaactga
    24661 agctgttcag agtggagtcg gcgttgcagt tcgtaatcct gcaacattgc attaatgcga
    24721 cgacgctgct cctgtacctg ctgctgttga ccattgtttg cggtcgcttc agaaggtact
    24781 cccaggctaa ccgggctggc tttacccatc atcggcagtg tattaaatac cggcgtttcg
    24841 ggctgctggg acgtttcaga ttgtccatta tagtgctgga cgccaacgat aactgcaagc
    24901 gatacgcatg cggctacgcc catttgggta agctgtgccg cccacggacg tactttctgc
    24961 cagaatggca ttttctgcca ttgatgcggc gcaggctggg cttccgggat caatgtcgcc
    25021 ggttgacgta ctggctcttc ttcaatggcg gccatcacgc gtgaagagat atcgaaatgg
    25081 agcacctcgg gagtatcacc ccgcattgag tcacggatta agtgatagct ttcccaggtt
    25141 ttctgcattt ctggattatg agccagttcg ttaagcagct cactatccag cgtttcgcca
    25201 tccattaaag cggaaagttg ttctttctgc atgcctaata cccttatcca gtatcccgct
    25261 atcgtcaacg cctgataagc ggttgaactt tgttatcaat agcttccctc gctcggaaga
    25321 tacgtgaacg cactgtacct accggacaat ccatgatagc ggctatctct tcatagctca
    25381 ggccatccag ctcccgcaag gttattgcca tgcgtaaatc ttccgggagg gactcaatag
    25441 ttcggaaaac tatctgtctc agttcttctg acaacattaa gttctcaggg ttcgaaattt
    25501 ctttcaacgc gccgccactt tcgaagtttt cagcttcaat ggcatccaca tcactggaag
    25561 gtggacgacg cccctgagca accaggtaat ttttcgctgt atttacagca atccgataca
    25621 gccatgtata aaaagcgcta tctccccgga acgaatccag cgcacgatag gctttaataa
    25681 aagcttcttg taccacatcg ggaacatcac ccgacggcac atagcgggaa accagactcg
    25741 ccactttatg ctgatagcgc actaccagta agttaaaggc tttctgatct cccttctgga
    25801 cccgttcaac caggacctgg tccgttaact gctcgctcat ccgaggtaaa gtctccccaa
    25861 accaaatttc cacgcgctat cgaaacgcca ctccattagc tgcaatttga gcaagcaaag
    25921 gattagagtg tctcgttttt gtaaagttcc gtaacgcatc tgtttttgtt tgtcatgctg
    25981 tagacggatc attatctatc attataagtc tacagaatct gaacatcgca ttatctgtgt
    26041 agaaatgccc atttaactgc ctgaagagta acccaacggc ctttttattt caccacctaa
    26101 tcctccacca gccagtaact tctctttttc tcgccgccct gcatcagcgt gtttagccac
    26161 tgtaacaaat attaaaatag caggtgttta ttcgcacaac atgatgctat gctgaccaaa
    26221 ccgtgtttag taaattaaac aaagaaaatg aatactctcc ctgaacattc atgtgacgtg
    26281 ttgattatcg gtagcggcgc agccgggctt tcactggcgc tacgcctggc tgaccagcat
    26341 caggtcatcg ttctaagtaa aggcccggta acggaaggtt caacatttta tgcccagggc
    26401 ggtattgccg ccgtgtttga tgaaactgac agcattgact cgcatgtgga agacacattg
    26461 attgccgggg ctggtatttg cgatcgccat gcagttgaat ttgtcgccag caatgcacga
    26521 tcctgtgtgc aatggctaat cgaccagggg gtgttatttg atacccacat tcagccgaat
    26581 ggcgaagaaa gctaccatct gacccgtgaa ggtggacata gtcaccgtcg tattcttcat
    26641 gccgccgacg caactggtag agaagtagaa accacgctgg tgagcaaggc gctgaaccat
    26701 ccgaatattc gcgtgctgga gcgcagcaac gcggttgatc tgattatttc tgacaaaatt
    26761 ggcctgccgg gcacgcgacg ggttgttggc gcgtgggtat ggaaccgtaa taaagaaacg
    26821 gtggaaacct gccacgcaaa agcggtggtg ctggcaactg gcggtgcgtc gaaggtttat
    26881 cagtacacca ccaatccgga tatttcttct ggcgatggca ttgctatggc gtggcgcgca
    26941 ggctgccggg ttgccaatct cgaatttaat cagttccacc ctaccgcgct atatcaccca
    27001 caggcacgca atttcctgtt aacagaagca ctgcgcggcg aaggcgctta tctcaagcgc
    27061 ccggatggta cgcgttttat gcccgatttt gatgagcgcg gcgaactggc cccgcgcgat
    27121 attgtcgccc gcgccattga ccatgaaatg aaacgcctcg gcgcagattg tatgttcctt
    27181 gatatcagcc ataagcccgc cgattttatt cgccagcatt tcccgatgat ttatgaaaag
    27241 ctgctcgggc tggggattga tctcacacaa gaaccggtac cgattgtgcc tgctgcacat
    27301 tatacctgcg gtggtgtaat ggttgatgat catgggcgta cggacgtcga gggcttgtat
    27361 gccattggcg aggtgagtta taccggctta cacggcgcta accgcatggc ctcgaattca
    27421 ttgctggagt gtctggtcta tggctggtcg gcggcggaag atatcaccag acgtatgcct
    27481 tatgcccacg acatcagtac gttaccgccg tgggatgaaa gccgcgttga gaaccctgac
    27541 gaacgggtag taattcagca taactggcac gagctacgtc tgtttatgtg ggattacgtt
    27601 ggcattgtgc gcacaacgaa gcgcctggaa cgcgccctgc ggcggataac catgctccaa
    27661 caagaaatag acgaatatta cgcccatttc cgcgtctcaa ataatttgct ggagctgcgt
    27721 aatctggtac aggttgccga gttgattgtt cgctgtgcaa tgatgcgtaa agagagtcgg
    27781 gggttacatt tcacgctgga ttatccggaa ctgctcaccc attccggtcc gtcgatcctt
    27841 tcccccggca atcattacat aaacagataa aaagcctggg tcagcgccgt atacgcttcg
    27901 gaatagttct ggtctggccc acgaatgact aagcgatcgc taaagcattc tcccgcctgc
    27961 ggggagaatg ccagcagcac ccgatgcggc agtcgcgctt cgttttccgc cacatccgtc
    28021 cgcaaacgta aatgccagcc catgcttaat gccagctccg taaaaccatt accaatctgc
    28081 tctggcagca ctacgcagaa aaatccctct tcggtaatgc actccgccgc acaggtcagc
    28141 aacgatgggt gatcaagcgt agtggtatag cgagcctgtt cccgttgagg tgtcgagcac
    28201 tctactccct gctgatagta aggtgggtta ctgatgatta aatcgaagcg tactgtctgc
    28261 tgtgtgatcc actgctgaat atccgccgta tggacgttaa tccgctctgc ccacggggac
    28321 tggttgatat tttcctgcgc ctgcgcggca gcttcacttt ccagttcaac tgcatcaatc
    28381 atcacgctgt catcggttcg ctgcgccagc attaatgcca gcaacccgct acccgcgccg
    28441 atatcaaggc aacgttttac cccagccacc ggtgcccatg cgcccaataa aataccatcc
    28501 gttcccactt tcatcgcaca gcgatcgtga gcaacaaaaa actgtttaaa agtaaatcca
    28561 ttacgacgaa gcacggatgt agactgtgac atgaaaataa aaccttgcag gaaaaacggc
    28621 gatagcaccg ggtgagaaca atacccgaga agcgatatcc atacaaacag atgaagattg
    28681 cagccgtaac gtctataatc agcgccccac acagaggtag aacatgactg taacgacttt
    28741 ttccgaactt gaactcgacg aaagcctgct ggaagccctc caggataaag gtttcactcg
    28801 cccgaccgcc attcaggctg ccgccattcc gcctgcgctc gatggccgtg atgtactcgg
    28861 ttctgcgccg acaggcaccg gtaaaacggc ggcgtatctg ctgccagcgt tgcagcacct
    28921 gctcgatttc ccgcgtaaga aatccggtcc gccgcgtatt ttgatcctca ccccaactcg
    28981 cgagctggcg atgcaggtgg ccgatcatgc ccgcgaactg gcgaaacata cgcatctgga
    29041 tatcgccacc atcaccggcg gcgtagccta tatgaaccac gcggaagtgt tcagcgaaaa
    29101 ccaggacatc gtggtcgcca cgaccgggcg tctgctgcaa tacataaaag aagagaactt
    29161 cgattgccgc gcggttgaaa cgctgatcct cgacgaagca gaccgtatgc tggatatggg
    29221 cttcgctcag gatatcgaac atattgctgg cgaaacgcgc tggcgtaaac agaccctgct
    29281 cttttcggca acgctggaag gcgatgcgat tcaggacttt gccgagcgtc tgctggaaga
    29341 cccggtggaa gtttctgcca atccgtccac ccgtgagcgc aaaaaaattc atcagtggta
    29401 ttaccgcgcc gatgatcttg agcataaaac cgcgttgctg gtgcatctgt taaaacagcc
    29461 ggaagcgacc cgctcaattg tgtttgtgcg taagcgtgaa cgtgtgcatg agctggcaaa
    29521 ctggctgcgc gaagcgggca tcaacaactg ctatctcgaa ggtgagatgg tacaaggcaa
    29581 gcgtaacgaa gcgatcaagc gtttgaccga aggtcgcgta aacgtactgg ttgcaaccga
    29641 tgttgccgcg cgcggtatcg acattcctga cgtcagccac gtctttaact tcgatatgcc
    29701 gcgcagtggt gatacttatt tgcaccgtat cggacgtacc gcgcgcgccg gtcgtaaggg
    29761 aacagcgatt tcgctggttg aagctcatga ccatctgctg ctgggtaaag taggccgcta
    29821 tattgaagag ccaattaaag ctcgcgttat tgatgagtta cgcccgaaaa cgcgtgcgcc
    29881 aagcgaaaag cagaccggca agccatcgaa gaaagtactg gctaaacgtg ctgagaagaa
    29941 aaaagctaaa gagaaagaga agccgcgggt gaaaaaacgc catcgcgaca ccaaaaatat
    30001 tggtaagcgc cgtaaaccaa gcggaacggg cgtgccaccg caaacgacag aagagtaatc
    30061 tcaatgccag gtttaagcct ggtattaaaa gtgctggcaa acgcaaaact gcctgatgcg
    30121 ctacgcttat caggcctacg tggctcatgc aatatattga atttgcacga tcttgtaggc
    30181 cggataaggt gttcacgccg catccggcat gaataaagcg cactttgtcg acaagttaaa
    30241 tgccgggttt aatcccgaca ttatttttat ccagccacaa aactctcttc aacacactga
    30301 ataaaagtgt gcatcgccgg gctaaccgct tttccagcat ggtgtgcgca catggccgta
    30361 atggtctgcg actgttcgcc aaaaggcaat tcaattaatt ctccgctctc aagctctttt
    30421 tccaccgcaa agcgcggtag gtaactgaca ccgatattcg ccgcgacaca acgtttgata
    30481 ctttcaatgc ttaacagctc aatggtgttt tccaccgtgc tccgccgctg gcgcaatgta
    30541 ctttcaaaga tctgccgaaa gacacattgc ggttcgttga taataaagct acaggcgtta
    30601 tgtcttcccg gctcagtaaa atcgacatct gcaatttgcg gtgaagccac cagcaccagc
    30661 gattgttcac ccaactctcg tcggttcagg gcatcatcat tccctacgcg ataaaagacg
    30721 ccgacatcgg cctcatcatt cagtagcgca tcacggatca cgtaacagtt cagcgactgc
    30781 aacgataaac gcacttttgg tgcgcgttgc cgaaaacgct gcaacacctg cggcattcgg
    30841 taagagagca acgtttcgcc cgaaacaacg cgtagttccc cgtccggatc tgactccttt
    30901 ttggcagcct cacgaagcgt atccatcact cgggtaagtt cataaatgtg cggcagcaac
    30961 ttttttcctt cgcgggtaag gcacatccgt cggccaattt tctcaaataa ctggactgaa
    31021 aactcctgct caagctgctg aatatgaaaa gtcaccgtcg attgtgtaca gcacaatttt
    31081 tgcgaagctc gcaaaaagga accctcttcc accacggttt taagcgtaat aaaacggcac
    31141 agatccataa ccccaaacct atcgaaaata tcgaatctag aatataaaaa cattcatttt
    31201 tttaaatgtt ccgtgtcggg tactgtctac caaaacagag gagataacaa gtgacaccga
    31261 cccttttaag tgctttttgg acttacaccc tgattaccgc tatgacgcca ggaccgaaca
    31321 atattctcgc ccttagctct gctacgtcgc atggatttcg tcaaagtacc cgcgtgctgg
    31381 cagggatgag tctgggattt ttgattgtga tgttactgtg tgcgggcatt tcattttcac
    31441 tggcagtgat tgacccggca gcggtacacc ttttgagttg ggcgggggcg gcatatattg
    31501 tctggctggc gtggaaaatc gccaccagcc caacaaagga agacggactt caggcaaaac
    31561 caatcagctt ttgggccagc tttgctttgc agtttgtgaa cgtcaaaatc attttgtacg
    31621 gtgttacggc actgtcgacg tttgttctgc cgcaaacaca ggcgctaagc tgggtagttg
    31681 gcgtcagcgt tttgctggcg atgattggga cgtttggcaa tgtgtgctgg gcgctggcgg
    31741 ggcatctgtt tcagcgattg tttcgccagt atggtcgcca gttaaatatc gtgcttgccc
    31801 tgttgctggt ctattgcgcg gtacgcattt tctattaacg aaaaaaagcg gaagaggtcg
    31861 ccctcttccg cttagtaact tgctacttaa gccttacagg ctttcagtaa aggtacgagc
    31921 gataacgtcg cgctgctgtt ccggagtcag agagttaaag cgaactgcat aaccggatac
    31981 acggatggtc agctgcggat atttttccgg atgcttaact gcatcttcca gagtttcgcg
    32041 acgcagaacg ttaacgttca ggtgttgacc accttcaacg cgaacttctg gtttcacttc
    32101 tactggaact tcacggtatt caatgtcacc cagtttgctt actgcaacca cttcatcttc
    32161 tgcataacct gcttttgcaa cgatgcaacg cgcttcgcct ttttcgctgt ccagcagcca
    32221 gaaagagttc agcagatcgt cgttagcggc tttagtaatc tggatacctg taatcatgtg
    32281 atgcctcccc ggcaaaatta tttgatttgt tcagcctgtc gcggccaatt ggtaaaacca
    32341 ttgttgcttg agtgtatata tactcctcaa acacccttga atctttgatt taaatcaata
    32401 aaaaccacac atcaagtatg gtcgcaaatg gattttattg ttttacatca acttatgcgg
    32461 gtgtgaaatt ttaccaattt acattttttt gcactcgttt aagtctaaaa aatgagcatg
    32521 attttgttct gtagaaagaa gcagttaagc taggcggatt gaagattcgc aggagagcga
    32581 gatggctaac gaattaacct ggcatgacgt gctggctgaa gagaagcagc aaccctattt
    32641 tcttaatacc cttcagaccg tcgccagcga gcggcagtcc ggcgtcacta tctacccacc
    32701 acaaaaagat gtctttaacg cgttccgctt tacagagttg ggtgacgtta aagtggtgat
    32761 tctcggccag gatccttatc acggaccggg acaggcgcat ggtctggcat tttccgttcg
    32821 tcccggcatt gccattcctc cgtcattatt gaatatgtat aaagagctgg aaaatactat
    32881 tccgggcttc acccgcccta atcatggtta tcttgaaagc tgggcgcgtc agggcgttct
    32941 gctactcaat actgtgttga cggtacgcgc aggtcaggcg cattcccacg ccagcctcgg
    33001 ctgggaaacc ttcaccgata aagtgatcag cctgattaac cagcatcgcg aaggcgtggt
    33061 gtttttgttg tggggatcgc atgcgcaaaa gaaaggggcg attatagata agcaacgcca
    33121 tcatgtactg aaagcaccgc atccgtcgcc gctttcggcg catcgtggat tctttggctg
    33181 caaccatttt gtgctggcaa atcagtggct ggaacaacat ggcgagacgc cgattgactg
    33241 gatgccagta ttaccggcag agagtgagta aatttgcggg aaaatgccgg atggcagagt
    33301 tgccacccgg ctgatttatc aggctttatt ctgacgccac cattcaccaa gcaaaacgcc
    33361 tgttgcaaca gagatattca gcccggcaac gttgcccgta ccgtcaatct tcacgcgcag
    33421 atcgttcgga tcgcgtgcgg catccggtaa cccttcatat tcttgaccca gcaccagtac
    33481 cattttcgct ggcaggctgg ttttgaacag cggtttcccc tgctcgctgg aagttgtcac
    33541 tacggtgtaa cccgcctgac ggaaatcatc cagcacgtta acaatgttgt cgccagtaat
    33601 cggctgaacg tgctctgcgc cgccttctgc ggtacggata gccgcacccg actccagcag
    33661 tgccgcatcc tgcaccacaa cgcctttcac gccgaagtgc gcgcagctgc gcatcatgcc
    33721 acccaggtta tgcgggttag attcgttttc cagtgccaga acacaatcct gcgcacctgc
    33781 ctggcttacc cactgctgca cggttgtgcc attacgcttt ttgatcagga agcaaacgcc
    33841 gccgtgatgt tccgtgcctg acgcttttgt caattccgct tcatccacca catggtacgc
    33901 tttgcggttt gccgccatcc agcgcaaggc ttctttaaaa cgtggcgtta cgctctggat
    33961 aaaccaggcg cgaacaatcg cttccggacg gctctggaac agtgcctgac atgcattttc
    34021 gccgtagacg cgggtttctt ccgcacgctg acgacgcaac acttctggat caataaaact
    34081 tttaccactg atgccaccgt gatcggcctt ttccggcgtc tcatcacccg gcgcgcggga
    34141 aaccgtgcgc cacggcgaat cttcccactt gcggtcacgg ggctgactct gtttgtcatc
    34201 gcgggcgggg cgacggccac cgtcagcacg agattttcct ggacgcccgc cccctttccc
    34261 ggtacgcggg ttgtgggtac gtttatcaga atcatcatca ctgcggacat acatcacttt
    34321 gaccttgccg cttttacctt tcatttcatc gttcatgctt ttctccacca gcgctgcgcg
    34381 aagcgcgcag attacccgaa gtccgcgcgg ttcgccatga tttcgtacca aagcctgcga
    34441 ctatcatacc tattgaataa aacagattgt tgtctggaac aatgtccccg ataatatgta
    34501 acatattaga aacataccgg cgtcgttgcc gataagtctc cttactcatc ccgaggttag
    34561 ttatgaatac cgtttgtacc cattgtcagg ccatcaatcg cattcccgac gatcggatcg
    34621 aagatgcggc aaaatgcgga cgctgcggtc acgacttgtt tgacggagag gtgattaatg
    34681 cgaccggtga aacgctcgac aaattgctga aggatgatct acctgtggtg atcgacttct
    34741 gggcaccgtg gtgcggcccc tgccgtaatt tcgcaccaat ttttgaagat gtcgcgcaag
    34801 agcgtagcgg taaagtgcgc tttgtgaaag tgaataccga agctgaacgt gaattgagca
    34861 gtcgctttgg aattcgtagt ataccgacga tcatgatttt caaaaacggt caggttgtcg
    34921 acatgcttaa tggcgcagta ccgaaagcgc cgttcgatag ctggctgaac gaatctcttt
    34981 aatcttaccg gggcgcatct tgtgccccgt tttctcctct gcgacaatgg cgttttttcg
    35041 acgctctctt atgaccgaaa acgctgttct ccagttacgc gccgagcgta ttgcgcgcgc
    35101 aacacgtcct tttcttgccc gcggtaatcg cgttcgtcgc tgccaacgct gtcttttgcc
    35161 agagaaatta tgtctctgtt cgacaattac cccagcacaa gcaaaaagtc gcttctgttt
    35221 gctgatgttc gacaccgagc caatgaagcc cagtaatacc gggcgtctga ttgctgatat
    35281 tttgcctgat accgttgcgt ttcaatggtc gcgtaccgaa ccctcgcagg atttgctgga
    35341 tctggtacaa aacccgtatt atcagccaat ggtggtcttt cccgcctcgt atgctgatga
    35401 gcaacgggaa gtgatcttca caccacctgc cggtaagcca ccgctgttta tcatgctcga
    35461 tggtacctgg ccggaagctc gcaagatgtt tcgtaaaagt ccgtatctgg ataatcttcc
    35521 cgtcatttcc gtcgatcttt cccggctttc tgcctatcgc ctgcgtgaag cccaggctga
    35581 aggccaatat tgtactgccg aggtagccat cgcactgtta gatatggccg gcgataccgg
    35641 ggcggcagca gggttaggcg agcattttac ccgctttaaa acacgctatc tggcaggaaa
    35701 aacgcaacat ctgggtagca tcacagcaga acagttagaa agcgtttaaa atcattcggt
    35761 cacttctgcg ggagaccggt atgagtcagc gaggactgga agcactactg cgaccaaaat
    35821 cgatagcggt aattggcgcg tcgatgaaac ccaatcgcgc aggttacctg atgatgcgta
    35881 acctgctggc gggaggcttt aacggaccgg tactcccggt gacgccagcc tggaaagcgg
    35941 tgttgggtgt gttggcctgg ccggatatcg ccagcttgcc ctttacaccc gaccttgcgg
    36001 ttttatgtac caatgccagc cgtaatcttg ctcttatgga agagctcggc gagaaaggct
    36061 gtaaaacctg cattattctt tccgccccgg catcgcaaca cgaagatctc cgcgcctgcg
    36121 ccctgcgcca taacatgcgc ctgcttggac caaacagtct gggtttactg gctccctggc
    36181 aaggtctgaa tgccagcttt tcgcctgtgc cgattaaacg cggcaagctg gcgtttattt
    36241 cacaatcggc tgccgtctcc aacaccatcc tcgactgggc gcaacagcgt gagatgggct
    36301 tttcctactt tattgcgctc ggcgacagcc tggatatcga cgttgatgaa ttgctcgact
    36361 atctggcgcg cgacagtaaa accagcgcca tcctgctcta tctcgaacag ttaagcgacg
    36421 cgcgacgctt tgtttcggcg gcccgtagtg cctcgcgcaa taaaccgatt ctggtgatta
    36481 aaagcggacg tagcccggcg gctcaacgtc tgttgaacac cacggcagga atggatccgg
    36541 cgtgggatgc ggctattcag cgtgccggtt tgttgcgggt acaggatacc cacgaactgt
    36601 tttcggcggt ggaaaccctt agccatatgc gcccgctgcg tggcgaccgg ttgatgatta
    36661 tcagcaacgg tgctgcgcct gccgcgctgg cgctggatgc cttatggtca cgcaatggca
    36721 agctggcaac gctaagcgaa gaaacctgcc agaaattgcg cgatgcattg ccagaacatg
    36781 tggcagtctc taacccgctc gatctgcgcg atgacgccag cagcgagcat tatgtcaaaa
    36841 cgctggacat tctgctccac agccaggatt ttgatgcgct gatggttatt cattcgccca
    36901 gcgccgctgc tcccgcaaca gaaagcgcgc aagtattaat tgaagcggta aagcatcatc
    36961 cccgcagcaa atatgtctct ttgctgacga actggtgtgg cgagcactcc tcgcaagagg
    37021 cacgacgttt attcagcgaa gccgggctgc cgacctaccg taccccggaa ggaaccatca
    37081 ctgcttttat gcatatggtg gagtaccggc gtaatcagaa gcaactacgc gaaacgccgg
    37141 cgttgcccag caatctgact tccaataccg cagaagcgca tcttctgttg caacaggcga
    37201 ttgccgaagg ggctacgtcg ctcgataccc atgaagttca gcccatcctg caagcgtatg
    37261 gcatgaacac gctccctacc tggattgcca gcgatagcac cgaagcggta catattgctg
    37321 aacagattgg ttatccggtg gcgctgaaat tacgctcgcc ggatattccg cataaatcgg
    37381 aagttcaggg tgtcatgctg tacctgcgta cagccaatga agtccagcaa gcggcaaacg
    37441 ctattttcga tcgcgtaaaa atgacctggc cgcaggcgcg ggtccacggc ctgttggtgc
    37501 aaagtatggc taaccgtgct ggcgctcagg agttgcgggt tgtggttgag cacgatccgg
    37561 tttttgggcc gttgatcatg ctgggtgaag gcggtgtgga gtggcgtcct gaagatcaag
    37621 ccgtcgtcgc actgccgccg ctgaatatga acctggcccg ctatctggtt attcagggga
    37681 tcaaaagtaa aaagattcgt gcgcgcagtg cgctacgccc attggatgtt gcaggcttga
    37741 gccagcttct ggtgcaggtt tccaacttga ttgtcgattg cccggaaatt cagcgtctgg
    37801 atattcatcc tttgctggct tctggcagtg aatttaccgc gctggatgtc acgctggata
    37861 tcgcgccgtt tgaaggcgat aacgagagtc ggctggcagt gcgcccttat ccgcatcagc
    37921 tggaagaatg ggtagaattg aaaaacggtg aacgctgctt gttccgcccg attttgccag
    37981 aagatgagcc acaacttcag caattcattt cgcgagtcac caaagaagat ctttattacc
    38041 gctactttag cgagatcaac gaatttaccc atgaagattt agccaacatg acgcagatcg
    38101 actacgatcg ggaaatggcg tttgtagcgg tacgacgtat tgatcaaacg gaagagatcc
    38161 tcggcgtcac acgtgcgatc tccgaccctg ataacatcga tgccgaattt gccgtgctgg
    38221 ttcgctcgga tctcaaaggg ttaggcttag gtcgacgctt aatggaaaag ttgattacct
    38281 atacgcgaga tcacggacta caacgtctga atggtattac gatgccaaac aatcgtggca
    38341 tggtggcgct ggcccgcaag ctcgggttta acgttgatat ccagctcgaa gaggggatcg
    38401 ttgggcttac gctaaatctt gcccaacgcg aggaatcatg agtaaggtac tggaaatgtt
    38461 gaccacttaa tcgggactgg tgttattatt gcccgcttat gtcgtctgca ttgcacagag
    38521 gacccttcaa tgaacagaga agaaatgcac tgtgatgttg tcaaaattta agcgtaataa
    38581 acatcaacaa caccttgccc aactacccaa gatttctcaa tcagttgatg atgtcgattt
    38641 cttttacgct cccgccgact tccgggagac gctgctggaa aaaatagcca gcgcgaagca
    38701 gcgcatttgc attgtcgccc tgtatctcga acaggatgac ggtggcaaag gcattctgaa
    38761 cgcgttgtat gaagctaaaa ggcagcgtcc ggaactggat gtgcgggtgc tggtcgactg
    38821 gcatcgtgca caacgtggac gcattggcgc tgcggcatct aacactaacg ctgactggta
    38881 ctgccgcatg gcgcaggaaa atccgggcgt agatgttccg gtttatggcg ttccaatcaa
    38941 tacccgtgaa gcccttggtg ttctgcactt taaaggcttt atcatcgacg atagcgtact
    39001 ttatagcggt gccagcctga acgatgttta cctgcatcag cacgataaat atcgctacga
    39061 ccgttatcat cttatccgta accgtaagat gtcagacatt atgtttgaat gggttacaca
    39121 gaatattatg aatggccgcg gcgttaatcg tctggatgat gttaatcggc caaaaagccc
    39181 ggaaatcaag aacgatattc gtctgttccg ccaggagctg cgtgatgccg cttatcattt
    39241 ccagggcgat gccgacaacg atcagctttc cgtaacgccg ctagtggggc tggggaaatc
    39301 gagtctgttg aacaagacca ttttccatct tatgccttgt gccgagcaga aactaaccat
    39361 ctgtacgcca tacttcaacc tgccagcaat ccttgtgcgc aatattatcc agttgctgcg
    39421 cgaagggaaa aaggtcgaaa ttattgttgg tgataaaacc gcgaacgact tctacattcc
    39481 ggaagatgaa cctttcaaga taattggcgc attgccttat ctctatgaga tcaatctgcg
    39541 tcgtttcctg agccgtttgc agtattacgt caatactgac cagttagtgg ttcggttatg
    39601 gaaagatgac gacaacacct atcacctgaa agggatgtgg gttgatgata agtggatgtt
    39661 gatcaccggt aataacctga acccgcgcgc ctggcgtctg gatctggaaa acgccatttt
    39721 gatccacgat ccacaactgg agcttgcgcc gcagcgagag aaagaactgg agctgatccg
    39781 cgagcatacc accatcgtta agcactatcg cgatctgcaa agtattgccg attatccggt
    39841 gaaggttcgt aaactcatcc gccgtttgcg ccgtatccgc atcgaccgat taattagccg
    39901 aatcctgtaa tcacaacccc gtcctgtacg gggtttgttt tttggaggcc acgttttgcg
    39961 tattcttttt gtctgttcgc tattgttact ttctggatgc agccatatgg ctaacgatag
    40021 ctggagcggg caggataaag ctcaacactt tatcgcctcg gcgatgcttt ccgccgccgg
    40081 aaatgaatat tcacagcatc aggggatgag ccgggatcac agtgccatgt ttggattgat
    40141 gttctctgtc agtttggggg cgtcaaaaga gctttgggat agccgccccg aagggagcgg
    40201 ctggagctgg aaggatttgg cctgggatgt cgccggtgca agcaccggct ataccgtctg
    40261 gcaactgacc cgtcactaaa gacgcatccc cttccctttg cgatgtagca tcaaagaaac
    40321 cagaaacgcc accacggcca tcaaggtcac ataccagaag aaggctgttt ccattcctat
    40381 tgatttcagc gacaacgcta cgtactccgc cgaaccacca aatatagcat tagcgaccgc
    40441 atatgacaga ccaacgccta atgcgcgaac ctgtgccggg aacatctcag ccttcagtat
    40501 tccactgatt gatgtataaa aactcactat cagcagggca cacatcacca gaccaaaagc
    40561 ggcataaggc gaggaaacgt tttgcaatgc tgagagaata ggaacggtaa aaatggctgc
    40621 cagcgaaccg aaacataaca ttgaggtacg gcgaccaatc ttatccgaca gcgcgccaat
    40681 gagtggttga ataagcatga atacaaacaa tgcggcagtc ataatgccac tcgccacgtt
    40741 ggcatgcatt cccgcagtat ttaccagata cttctgcata taagtagtga aggtatagaa
    40801 acaaagggag cccgcagcgg taaaaccgag aaccatgatg aatgcacggc gattgcgcca
    40861 taatcctttc agagatccag cttcttttaa agcgcgcgtt tcttgttgcg aagtttcatc
    40921 taactgacga cgtaaccaca acgccacaac agctaacaca gctcctaacg cgaaaggaat
    40981 acgccatccc cactctctga gtgcagcgtc ttccatggtg tgttgtaaaa ccacgacaac
    41041 cagtagggct agcagttgtc cgccgatcaa cgtcacatac tgaaatgatg cgtaaaaacc
    41101 tttgcgccct tcaacggcaa cttcactcat ataggtggcg ctggtgccat attctccgcc
    41161 aacggataat ccctgaaata aacgagcgag aagcaataat gccggagccc acgtacctat
    41221 agtttcataa cctgggaggc aggcgataac cagcgatccg aaacacatca tacacaccga
    41281 taacagcatc gattttttgc gaccatgttt atcggctatg cggccaaata gccaaccgcc
    41341 tattgggcgc atcaggaatc ccgcagcaaa aacacctgct gtttgtagta gttgagtcgt
    41401 cgtgttcccg gaagggaaga aaatgtgggc gaagtagagt gaacagaacg agtagacata
    41461 gaaatcgaac cactcgacca gattacctga agaggccccc acaatcgccc aaatgcggcg
    41521 acgagtatca ctacttgtca gtttgctgtc tgccgttaca gtactttcag ccatgccatt
    41581 atgtctcctg ccgtaatccg atgcttttgt tggtcgcttt tgtttatttc ttttggtaaa
    41641 gaaaatgtta tacatttgtt gcgtatcatt atgcaacctt aaccatgaat ttagttagca
    41701 ggaaaatggt tattgaggag cttaaggata aatttctggt aaggaggaca cgtatggaag
    41761 taggcaagtt ggggaaaccg catccgttgc tgaatctggc atatgtggga gtataagacg
    41821 cgcagcgtcg catcaggcat ttttttcacc aacgcaaaaa ggcccagtct ttcgactgag
    41881 ccttttg
//
LOCUS       DACAPB010000014        41561 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.014, whole genome shotgun sequence.
ACCESSION   DACAPB010000014 DACAPB010000000
VERSION     DACAPB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.014"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            47..121
                     /locus_tag="IT904_000692"
     tRNA            47..121
                     /locus_tag="IT904_000692"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79..81,aa:Gln,seq:ctg)
     gene            complement(275..1450)
                     /gene="ubiF"
                     /locus_tag="IT904_000693"
     CDS             complement(275..1450)
                     /gene="ubiF"
                     /locus_tag="IT904_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAP3190522.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            1596..3020
                     /gene="miaB"
                     /locus_tag="IT904_000694"
     CDS             1596..3020
                     /gene="miaB"
                     /locus_tag="IT904_000694"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAP3190523.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3134..4213
                     /locus_tag="IT904_000695"
     CDS             3134..4213
                     /locus_tag="IT904_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP3190524.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDIAFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4210..4677
                     /gene="ybeY"
                     /locus_tag="IT904_000696"
     CDS             4210..4677
                     /gene="ybeY"
                     /locus_tag="IT904_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP3190525.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4767..5645
                     /gene="corC"
                     /locus_tag="IT904_000697"
     CDS             4767..5645
                     /gene="corC"
                     /locus_tag="IT904_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAP3190526.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5670..7208
                     /gene="lnt"
                     /locus_tag="IT904_000698"
     CDS             5670..7208
                     /gene="lnt"
                     /locus_tag="IT904_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAP3190527.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7335..9211)
                     /locus_tag="IT904_000699"
                     /pseudo
     CDS             complement(7335..9211)
                     /locus_tag="IT904_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            9666..10574
                     /gene="gltI"
                     /locus_tag="IT904_000700"
     CDS             9666..10574
                     /gene="gltI"
                     /locus_tag="IT904_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAP3190528.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10744..11484
                     /gene="gltJ"
                     /locus_tag="IT904_000701"
     CDS             10744..11484
                     /gene="gltJ"
                     /locus_tag="IT904_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAP3190529.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11484..12158
                     /gene="gltK"
                     /locus_tag="IT904_000702"
     CDS             11484..12158
                     /gene="gltK"
                     /locus_tag="IT904_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAP3190530.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12158..12883
                     /gene="gltL"
                     /locus_tag="IT904_000703"
     CDS             12158..12883
                     /gene="gltL"
                     /locus_tag="IT904_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAP3190531.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13001..13936
                     /gene="rihA"
                     /locus_tag="IT904_000704"
     CDS             13001..13936
                     /gene="rihA"
                     /locus_tag="IT904_000704"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAP3190532.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHRAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(13976..14458)
                     /locus_tag="IT904_000705"
     CDS             complement(13976..14458)
                     /locus_tag="IT904_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAP3190533.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLMLCPKCGHDQFQRRPFEP
                     "
     gene            14693..17275
                     /gene="leuS"
                     /locus_tag="IT904_000706"
     CDS             14693..17275
                     /gene="leuS"
                     /locus_tag="IT904_000706"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP3190534.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNNYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVATLNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17290..17871
                     /gene="lptE"
                     /locus_tag="IT904_000707"
     CDS             17290..17871
                     /gene="lptE"
                     /locus_tag="IT904_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAP3190535.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            17871..18902
                     /gene="holA"
                     /locus_tag="IT904_000708"
     CDS             17871..18902
                     /gene="holA"
                     /locus_tag="IT904_000708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP3190536.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENSAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            18904..19545
                     /gene="nadD"
                     /locus_tag="IT904_000709"
     CDS             18904..19545
                     /gene="nadD"
                     /locus_tag="IT904_000709"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP3190537.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19569..20180
                     /locus_tag="IT904_000710"
     CDS             19569..20180
                     /locus_tag="IT904_000710"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAP3190538.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIFSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            20440..20757
                     /gene="rsfS"
                     /locus_tag="IT904_000711"
                     /gene_synonym="rsfA"
     CDS             20440..20757
                     /gene="rsfS"
                     /locus_tag="IT904_000711"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP3190539.1"
                     /translation="MQGKALQYFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20761..21228
                     /gene="rlmH"
                     /locus_tag="IT904_000712"
     CDS             20761..21228
                     /gene="rlmH"
                     /locus_tag="IT904_000712"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP3190540.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21259..23160
                     /gene="mrdA"
                     /locus_tag="IT904_000713"
     CDS             21259..23160
                     /gene="mrdA"
                     /locus_tag="IT904_000713"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAP3190541.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23163..24275
                     /gene="mrdB"
                     /locus_tag="IT904_000714"
                     /gene_synonym="rodA"
     CDS             23163..24275
                     /gene="mrdB"
                     /locus_tag="IT904_000714"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAP3190542.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24286..25374
                     /gene="rlpA"
                     /locus_tag="IT904_000715"
     CDS             24286..25374
                     /gene="rlpA"
                     /locus_tag="IT904_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAP3190543.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPCFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25514..26725
                     /gene="dacA"
                     /locus_tag="IT904_000716"
     CDS             25514..26725
                     /gene="dacA"
                     /locus_tag="IT904_000716"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAP3190544.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILLDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26836..27099
                     /locus_tag="IT904_000717"
     CDS             26836..27099
                     /locus_tag="IT904_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAP3190545.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27200..27841
                     /gene="lipB"
                     /locus_tag="IT904_000718"
     CDS             27200..27841
                     /gene="lipB"
                     /locus_tag="IT904_000718"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAP3190546.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            28100..29053
                     /locus_tag="IT904_000719"
     CDS             28100..29053
                     /locus_tag="IT904_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP3190547.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            29262..30227
                     /gene="lipA"
                     /locus_tag="IT904_000720"
     CDS             29262..30227
                     /gene="lipA"
                     /locus_tag="IT904_000720"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAP3190548.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(30328..30531)
                     /gene="tatE"
                     /locus_tag="IT904_000721"
     CDS             complement(30328..30531)
                     /gene="tatE"
                     /locus_tag="IT904_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAP3190549.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(30660..31448)
                     /locus_tag="IT904_000722"
     CDS             complement(30660..31448)
                     /locus_tag="IT904_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAP3190550.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            31541..31924
                     /gene="crcB"
                     /locus_tag="IT904_000723"
     CDS             31541..31924
                     /gene="crcB"
                     /locus_tag="IT904_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAP3190551.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(31979..32188)
                     /gene="cspE"
                     /locus_tag="IT904_000724"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(31979..32188)
                     /gene="cspE"
                     /locus_tag="IT904_000724"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP3190552.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(32363..32923)
                     /locus_tag="IT904_000725"
     CDS             complement(32363..32923)
                     /locus_tag="IT904_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAP3190553.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            33512..34897
                     /gene="dcuC"
                     /locus_tag="IT904_000726"
     CDS             33512..34897
                     /gene="dcuC"
                     /locus_tag="IT904_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAP3190554.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(34938..35618)
                     /gene="dpiA"
                     /locus_tag="IT904_000727"
                     /gene_synonym="citB"
     CDS             complement(34938..35618)
                     /gene="dpiA"
                     /locus_tag="IT904_000727"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAP3190555.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(35587..37245)
                     /gene="dpiB"
                     /locus_tag="IT904_000728"
     CDS             complement(35587..37245)
                     /gene="dpiB"
                     /locus_tag="IT904_000728"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HAP3190556.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKTIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            37625..38683
                     /gene="citC"
                     /locus_tag="IT904_000729"
     CDS             37625..38683
                     /gene="citC"
                     /locus_tag="IT904_000729"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP3190557.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            38698..38994
                     /gene="citD"
                     /locus_tag="IT904_000730"
     CDS             38698..38994
                     /gene="citD"
                     /locus_tag="IT904_000730"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP3190558.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            38991..39899
                     /gene="citE"
                     /locus_tag="IT904_000731"
     CDS             38991..39899
                     /gene="citE"
                     /locus_tag="IT904_000731"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP3190559.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            39910..41442
                     /gene="citF"
                     /locus_tag="IT904_000732"
     CDS             39910..41442
                     /gene="citF"
                     /locus_tag="IT904_000732"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP3190560.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            41446..>41561
                     /gene="citX"
                     /locus_tag="IT904_000733"
     CDS             41446..>41561
                     /gene="citX"
                     /locus_tag="IT904_000733"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="2'-(5''-phosphoribosyl)-3'-dephospho-CoA
                     transferase; holo-citrate lyase synthase; CitG forms the
                     prosthetic group precursor
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA which is then
                     transferred to apo-ACP by CitX to produce holo-ACP and
                     pyrophosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP3190561.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVP"
BASE COUNT        10191 a        10818 c        10420 g        10132 t
ORIGIN      
        1 attctgaatg tgtcgaatat attcggcaaa ttcaaaacca atttgttggg gtatcgccaa
       61 gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc
      121 acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgccgaat
      181 gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac
      241 ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg
      301 cctgacgttt caacacgcca gcacgctccg ccgccattaa cccgagatta cgcataaaac
      361 gcagtggtgg cagattattg ctgaatccgg cataaaacag atccataccg ctttgcataa
      421 tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg
      481 cttcgccgta gctgcgggcg ttgaccagaa catcaatcag ggcatcgaca tcacgataac
      541 caagattcac tccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg
      601 caagccctgg ctgcacgtac tgcaacgcat ggcgacgcgt cagcggaaac gcaccagcgg
      661 caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagct
      721 gtgccatatt catattctgc aactggcgaa tacgcgccgg agagtcatac cacaccagcg
      781 atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggagta aattgctgcc
      841 aggtgctgtc gccgggatcg ttctcgcact ggacgctaat caacatgcac gactgcgcat
      901 actgccatgc atgaacgcca attcccgcca tctgccgcac ctgcgaattt gcgccgtcgg
      961 caccaatcac cagcttcgcg cgaatcactt caccgccttt cagctccagc tcctgaagat
     1021 catcatggcg atgcagcgca atcagcgagc ctggcacacg taacgttact ttcggatgcg
     1081 cttccagcgc ctgccacaac gcctgttgca ggacagtgtt ttccaccata tagccaagca
     1141 gcggtagctt aagttcagcg gcgtcaaaca ccacatgcgc cgtttcccac tcccacgttt
     1201 ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc cctaaccctt
     1261 taagcaatga taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctatcag
     1321 cgacaaacgg cgctggttct gcgtgctcga tcaccgttac cgcaaatccg tgctgtgcca
     1381 gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt
     1441 gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc
     1501 ctaagggctg ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc
     1561 tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa
     1621 cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca
     1681 cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctgctg ctgaacacct
     1741 gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggccgc tggaaactgt
     1801 taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag
     1861 gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc
     1921 accgtctgcc ggagatgatc aactccgtgc gtggcgaccg cagcccggtt gtagatatca
     1981 gcttcccgga aatcgagaag tttgaccgtc taccggaacc gcgcgccgaa gggccgaccg
     2041 cgtttgtctc catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca
     2101 cccgtggtga agaggtaagc cgtccgtccg acgatattct gtttgagatt gcccagcttg
     2161 cggctcaggg cgtgcgtgaa gtcaatctgc tcggtcagaa cgtgaacgcc tggcgtggtg
     2221 aaaactacga cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg
     2281 acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca
     2341 tcgaagtgta ccgcgacacg ccggagctgg tgagcttcct gcatctgccg gtacagagcg
     2401 gttccgatcg cattctgaac ctgatgggcc gtactcatac ggcactggag tacaaagcga
     2461 tcatccgtaa actgcgtgcg gcgcgtccag atattcagat cagttctgat ttcatcgttg
     2521 gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca
     2581 atttcgacat gagctacagc tttatcttct ctgcacgtcc gggtacacca gccgccgata
     2641 tggttgatga tgttccggaa gaagagaaga agcaacgtct gtatattctg caagaacgta
     2701 tcaatcagca agcgatggca tggagccgcc ggatgctcgg caccacccag cgtattctgg
     2761 tagaagggac ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa aataaccgcg
     2821 tggtcaactt cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg
     2881 acgtctaccc gaactctctg cgcggtaaag tggtgcgtac tgaagatgaa atgggtctgc
     2941 gcgtggcaga aacaccggaa tcggtgattg cccgtacccg caaagaaaac gaccttggcg
     3001 tgggttatta tcagccgtaa ttctcaggcc cgccgttccg gtgggccttg ttttttctct
     3061 ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct
     3121 tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat
     3181 agacactcgc gaaatcaccc tggagccagc agacaacgcg cgtctgttga gcctgtgcgg
     3241 cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg
     3301 cgacaaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct
     3361 gcgtagcctg tatgtcgata cagccccgat gcgcggtcag attcaggata tcgaaccgga
     3421 acagatccac cttgcgatta aagaagcaag ggtactggag caaagcgcgg agagcgtgcc
     3481 ggagtacggc aaagcggtca atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc
     3541 aaaccaggcg cagtacatcg ccaatattct cgaccatgac attgccttcg gcgttggccc
     3601 ggcgggtacg ggtaaaacct acctggcagt cgctgcagca gttgatgccc tggagcgtca
     3661 ggaaattcgc cgtattctgc tgactcgtcc agcggtcgaa gccggtgaga aactgggctt
     3721 cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccactgt acgacgcgct
     3781 gtttgaaatg ctgggctttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt
     3841 cgcgccgctg gcctatatgc gtggtcgtac gctgaatgac gcctttatca ttctcgatga
     3901 gagccagaac accaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc
     3961 aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg
     4021 cttacgtcat gccatcgaag tgctggccga tgtcgaagag atcagcttta acttcttcca
     4081 cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc gttaacgcct atgaagcctg
     4141 ggaagaagcc gaacaaaaac gtaaagcggc gctggcggca gaacgcaagc gcgaagaaca
     4201 ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg
     4261 ttaccggaag agagccagtt tcagacatgg ctgaatgcgg tgatcccgca gtttcaggaa
     4321 gagtcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct gaatctgacc
     4381 tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc
     4441 atggagatgt cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct
     4501 caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg
     4561 catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga agccctcgaa
     4621 acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt
     4681 gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta
     4741 actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga
     4801 taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga
     4861 aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg
     4921 aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca
     4981 tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc
     5041 ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc
     5101 acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag
     5161 ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag
     5221 accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat
     5281 tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactgatt gttggtgaga
     5341 ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct
     5401 ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta
     5461 gcgatgaaga agtcgacact atcggtggtc tggtgatgca ggcgtttggg catcttccgg
     5521 cgcgtggcga aactatcgac atcgacggtt accagttcaa agtggcgatg gccgatagtc
     5581 ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagcca aagctggatg
     5641 aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc
     5701 gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt
     5761 acgacgtctg gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc
     5821 gtcgcccact ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg
     5881 gtattaactg ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca
     5941 tcttcctggt ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg
     6001 tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgct gcccctgccc
     6061 tctggcaagt gaccgagttt ctgcgcggtt gggtactgac cggcttcccg tggttacagt
     6121 tcggctatag ccagattgat ggcccgttaa aagggctggc accgataatg ggcgtggaag
     6181 ccattaactt cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca
     6241 actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca
     6301 tccaatggtt taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata
     6361 tcccacaatc gctgaaatgg gacgaaggcc agcttcttaa tacgctgaag atttactaca
     6421 acgcaacggc accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa
     6481 ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata
     6541 aaggtagctc gctggtcacc gggattgtcg acgcgcgtct caataagcag aaccgctacg
     6601 atacctacaa caccatcatc acgctgggta aaggtgcacc gtacagctac gaatcagccg
     6661 atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc
     6721 tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata
     6781 tccagccgcc gctgtcggta aatggtattg agcttactgc ggctatttgc tacgagatca
     6841 ttctcggcga gcaagtgcgc gataacttcc gcccggatac cgactacctg ctgactatct
     6901 ccaacgatgc atggtttggt aagtctattg gtccatggca gcacttccag atggcgcgaa
     6961 tgcgtgcgct ggagctggcg cgcccattgc tgcgtagcac caacaacggc attacggcgg
     7021 tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttga
     7081 ccactaacgt gacgccgacg accggactca caccgtacgc acgaaccggc aactggccgc
     7141 tgtgggtgct gacggcgctg tttggttttg ccgctgtgct gatgagtctg cgagcacgta
     7201 aacgttgatg tgcctgatgc gctacgctta tcaggcctac gcgatttttg caatatgttg
     7261 aatttacgtg attttgtagg ataaagcgta ctttctcaac acgctgtggg gcatgattat
     7321 catgcccctt tttcttacgg ttcagggata caagccacat tgccatattg gggatgacga
     7381 ataactgcct gcggatcaag cgagagcgtc ctgtccattt ggattaaccc gttcttcgcc
     7441 ataacaaata tctttctacc atccgccgaa aaacgaaggt gattaacgta gctggcagga
     7501 taaacgccaa caatctgccc gacattaata ttgagcagga tgacataacc ttgcaagcca
     7561 tatgacacca acgctaaata ctgaccgtcc gggctaatac cctcaacgga ataaacgctc
     7621 ctgctacacc attgcgtcat aatgctggct tgctgatccc caactcttat acgtgaaatc
     7681 ccctggctgt cagcaaaaat tacatccccg ttttcaggtc gaaaacgcat cgccgatatc
     7741 gttccacgcg catcacctgc gggaaagtct tctttctgat gcgtgatggt attgatgcgt
     7801 acaatgtcac ctctgaccat accgggtatc aacaatgttt tttcatccgg ggaaagtgcc
     7861 ataacccagg caccaggttt accgctcgtc cccgtcccgt cgtatttctc cagcgggata
     7921 tccgccacat gctgataagt caccagatca atcgctgaca cactattact ggtgcgattc
     7981 atcacccagg cttttttgcc atcacgcgag agtataaggc tgtcaggatg tgcaccggtg
     8041 gtaatcgact gaataatctc ccccgtcgcc acgttaatga ccgataacgc attcgcttca
     8101 aatgaagtcg cataaagcaa cttttcatcc gggcttaacg ccagcgatct gacgccctga
     8161 tctttccagc caccacaatg tttgactgac ttttctttcg ctatgggcgc tgtaaaggta
     8221 tgcaggattt tcttttgtcc gaggtcgaac acaacaatgc tgtttgtgtt ttcaatagcg
     8281 gcatataacg tattaccatc ccgactgatc gccatatcat ggatatcggc aatctcagcg
     8341 agcaaatcac cactggcctg ttgacgactt accggagcgg gtaatgtttc tttgagggcg
     8401 ttgcgttcct ccgctaactg ttgctttttt gtcgtcgctc ttgttcaatc tcctgcggat
     8461 aaaacgcttc acgcaggctt tgcctgacct gtaaaactga ttcatcaatc tgctgctgcg
     8521 caagccagat agccccggta agaagaagac tgcccgcaac aataatcccg ccttccgtaa
     8581 caagccggtt ttgcccagat aaacgcgcac tcagccaacc caacgcgcca cccgcaatca
     8641 ggccgccaat gtgacaagca ttatctattc ctgtttgccg ggcaccatac aacagtgtca
     8701 gcgccaccat ccctaacaga gaaaatactc gccgctgatt tttatggtgc gtgcccgtac
     8761 ctgaagcaag ctgtgttgct atcgcagcgc cagctattcc cataattgct ccggatgccc
     8821 caacgctgat gactatctgc tcactatcgc gtaacgcata acaggcgctg atgagcccac
     8881 caaagacgcc ggagaataac cagataatca ggagtcgcca tttccccagt atccgttccg
     8941 ccactgcccc caccaccagg aatgcaaaca tattcatcag cagatgagaa acactgctgt
     9001 gtagaaagac actgctcaat aaacgccagg gttcacccga caacgtgaga gaagcaacgt
     9061 tagcgccgag atggataagt gcggattgat cgacttcttc ataactcatc cattgtgcga
     9121 ggtaacaaaa tatcccaaca cagagcgcaa aaaggataag ggttattgcg ggtaattgaa
     9181 catttaacgg cttaaccgaa gatgcagaca taagatgatt cttccttgaa aagacaggta
     9241 aaaagcaatt ttcccgtgtg cagttatcat cccggcaaac gtcccatacc gaaaagttaa
     9301 ccctgtccga aatatgactt tttcagcctg aaattacaat cgtaattaat tcacttctaa
     9361 gtattttttg gcatgtaagt tgctttattt actgtgtaag caatgaaatc aaccgcgacg
     9421 caaccgggtt gcaccaccac aaatcagcgg tagcaccaaa aaggtgcggt ttaagaattt
     9481 tgcttcggaa tggtgcggcg catctcgcaa aaacaaacaa taaaacagca atatctttac
     9541 atttagccaa accaaatgtt acctaatagg cacaacactg cacaataaag ttgcagacga
     9601 taacaacaca aacactcaca acgggtatcc atgcgttctt aacgcagaag ataaaggagt
     9661 tggatatgca attacgtaaa cctgccacag caatcctcgc cctggcgctt tccgcaggac
     9721 tggcacaggc agatgacgcc gccccggtag cgggcagtac gctggacaaa atcgccaaaa
     9781 acggcgtgat tgtcgtcggt caccgtgaat cttcagtacc tttctcttat tacgacaatc
     9841 agcaaaaagt ggtgggttac tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa
     9901 agaaactcaa caaaccggac ttgcaggtaa aactgattcc gattacctca caaaaccgta
     9961 ttccactgct gcaaaacggc actttcgatt ttgaatgtgg ttctaccacc aacaacgtcg
    10021 aacgccaaaa acaggcggct ttctctgata ctattttcgt ggtcggtacg cgcctgctga
    10081 ccaaaaaggg cggcgatatc aaagattttg ccgacctgaa aggcaaagcc gtagtcgtca
    10141 cttccggcac tacttctgaa gttttgctca acaagctgaa tgaagagcaa aaaatgaata
    10201 tgcgcatcat tagcgccaaa gatcacggtg actctttccg taccctggaa agcggtcgtg
    10261 ccgttgcctt tatgatggat gacgctctgc tggccggtga acgtgcgaaa gcgaagaaac
    10321 cagacaactg ggatatcgtc ggcaagccgc agtctcagga agcctacggt tgcatgttgc
    10381 gtaaagatga tccgcagttc aaaaagctga tggatgacac tatcgctcag gtgcagactt
    10441 ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa tccaattccg ccgaaaaacc
    10501 tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca
    10561 aagcactgaa ctaattacaa gaaccagggg cggaaattct agccctctcg attgttacgt
    10621 agcacggaca gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg
    10681 ctgggtaaca atcttcgagg gtagcagtta acgctgctac cctctttttt ctggagtaga
    10741 tttatgtcta tagactggaa ctggggtatt tttttacaac aagccccgtt cggcaacacc
    10801 acctatcttg gttggatctg gagtggtttt caggtgacga tcgctttgtc gatctgcgcc
    10861 tggattatcg ctttcctcgt cggttcattt ttcggcattt tacgtaccgt tccaaaccgt
    10921 tttctctccg gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc gttgattgtg
    10981 caattcttta cctggtatct ggtgatcccg gagctgctgc cggagaaaat cggcatgtgg
    11041 tttaaggccg agctggatcc caatattcag tttttccttt catccatgct ctgcctgggg
    11101 ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg ctattcagtc gctgccgcgt
    11161 gggcagaaga atgccgcgct ggcgatgggt ctgacgctac cgcaggctta ccgctacgtg
    11221 ctgttgccta acgcgtatcg cgttatcgtc ccgccaatga cttccgagat gatgaacctg
    11281 gtgaaaaact ccgccatcgc ctcgactatc ggtctggtgg atatggcagc gcaggcgggt
    11341 aaattgctcg actactcggc ccacgcctgg gagtcgttta ccgccattac gcttgcgtat
    11401 gtgttgatta acgcttttat catgctggtg atgacgctgg ttgaacgtaa agttcgcctg
    11461 cccggcaaca tggggggcaa ataatgtacg agtttgactg gagttccatt gttccttccc
    11521 tgccatatct gctcgacggg ctggtgatca ccctgaaaat caccgtcacg gcagtcgtga
    11581 ttggtatttt gtggggtacc atgctggcgg tgatgcgctt gtccagcttc gcgcctgttt
    11641 cgtggtttgc caaagcctac gttaacgtat tccgctcgat tcctttagtc atggttttgc
    11701 tgtggtttta cttgattgtg ccgggatttc tgcaaaatgt gctgggatta tcgccaaaaa
    11761 atgatattcg tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg gcctactatt
    11821 cagagattat ccgtgccggt attcaaagta tttctcgtgg tcagtcgagc gccgcgctgg
    11881 cgttgggaat gactcactgg cagtcgatga aactgattat tctgccgcag gcgttccgcg
    11941 cgatggtgcc gctgctgctc actcagggca tcgtactgtt ccaggatacc tcgctggtgt
    12001 atgtgttatc tctggccgat ttcttccgta ccgcctcaac cattggtgag cgtgatggta
    12061 cgcaggttga gatgatcctg tttgccggat ttgtttattt cgttattagt cttagcgcgt
    12121 cgttgttggt cagctacttg aaaagaagga cagcataatg attaccctga aaaatgtttc
    12181 aaaatggtat ggtcactttc aggtgctgac cgactgctca accgaagtga aaaaaggcga
    12241 agtggtggtg gtttgcggcc cgtctggttc cggcaaatca acgctgatta aaaccgtcaa
    12301 cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat ggtatcgtgg ttaatgacaa
    12361 gaaaaccgat ctggcaaagc tgcgttcccg cgtcgggatg gtattccaac atttcgagct
    12421 gttccctcat ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag tgcttaaacg
    12481 cgataaagca ccggctcgtg aaaaagcatt gaaattgctg gagcgcgtgg gactttccgc
    12541 acatgccaat aaatttccgg cgcagctttc cggcggccag cagcagcgtg tggcgatcgc
    12601 tcgcgcattg tgtatggatc ctattgccat gctgttcgac gaaccgacat cggcgctgga
    12661 tccggagatg atcaacgaag tgctggacgt aatggtcgaa ctggcgaacg aaggaatgac
    12721 catgatggtg gtgacccacg aaatgggctt tgcccgtaaa gtggcgaatc gggtgatctt
    12781 tatggacgag ggtaaaattg tcgaagactc gccgaaagac gctttcttcg atgatccgaa
    12841 atcggaccgc gcaaaagatt tcctcgcgaa aatcctgcat taatcactct ggcgcgcata
    12901 cccaattgcg cgccatctca cgctttatcg tttcgcccct cgtatctgtt acatcctggc
    12961 gttacccttg tcgcaaagaa gcacacaaca aggagcaaca atggcactgc caattctgtt
    13021 agattgcgac ccaggtcatg acgacgctat cgcaatagtt ctcgccctcg cctcaccaga
    13081 gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag acaccagaaa aaaccttacg
    13141 caatgttctg cgtatgctga ccttgcttaa tcgcaccgat attccggtag caggcggcgc
    13201 ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat gtgcacggcg aaagcggtct
    13261 cgacggcccg gcattaccgg aaccggcgtt cgcaccgcaa aactgtacgg cggtagagct
    13321 aatggcgaaa acgctgcgtg aaagtgcgga acctatcacg attgtgtcta ccggaccgca
    13381 aactaacgtt gccttgctgc tcaatagcca tccggaactg catagcaaaa ttgcccgtat
    13441 cgtgattatg ggcggcgcaa tggggctggg taactggacg cctgcggctg aatttaatat
    13501 ttacgttgac ccggaagcgg cagaaattgt cttccagtca gggatcccgg tggtgatggc
    13561 cggtctggat gttactcaca gagcacaaat ccacgttgaa gacactgagc gtttccgcgc
    13621 gattggcaac cctgtttcaa ccattgttgc cgaactgctg gatttcttcc tcgaatatca
    13681 taaagacgaa aaatggggct ttgtcggcgc accgctgcat gacccatgca ccatcgcctg
    13741 gctgttgaaa ccggagttgt ttaccactgt tgagcgctgg gttggcgtgg aaacacaagg
    13801 gaaatatacc cagggtatga cggttgttga ttattattat ctgactggca ataaaccgaa
    13861 tgcaactgta atggtcgatg ttgatcgtca gggctttgtt gatttactgg ctgatcgtct
    13921 gaaattttac gcttaacaac tcgccggatg acattacgtc atccggcacc attcattacg
    13981 gctcgaacgg gcgtctctgg aactgatcat gaccacattt cgggcatagc atcagcactt
    14041 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccta
    14101 ccacttctcc gctgtgataa accccgtgat gattgaggtc ctggaaaact tcgcgccatt
    14161 caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct ttaatcaccc
    14221 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
    14281 ccaggtcacg tctgacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
    14341 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattac
    14401 gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
    14461 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
    14521 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
    14581 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
    14641 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
    14701 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
    14761 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
    14821 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
    14881 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg
    14941 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgcg ccagcaccgt ggacgtacga
    15001 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
    15061 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
    15121 gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc cgaacgacca
    15181 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
    15241 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
    15301 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
    15361 gatcggtcgt tccgaaggcg tggaaatcac cttcaacgtt aacaactatg acaacacgct
    15421 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagcggc
    15481 gggtcatccg ctggcgcaga aagcggcaga aaataatcct gaactggcag cctttattga
    15541 cgaatgccgt aataccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
    15601 cgatactggc tttaaagcgg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
    15661 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
    15721 gcgcgactac gagtttgcct ctaaatatgg cctgaacatc aagccagtta ttctggcggc
    15781 tgacggttct gaaccggatc tctcccagca agccctgact gaaaaaggcg tgctgttcaa
    15841 ctctggcgag ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
    15901 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
    15961 tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
    16021 gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
    16081 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
    16141 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
    16201 cacttgcccg cagtacaaag aaggtatgct ggattctaaa gcggctaact actggctgcc
    16261 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
    16321 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
    16381 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
    16441 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
    16501 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
    16561 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
    16621 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggcgt
    16681 agaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtgtacgagc atacagcaaa
    16741 aggtgatgtt gcgacactga acgttgatgc actgactgaa gatcagaaag cgctgcgtcg
    16801 tgatgtgcat aaaactatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
    16861 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac cgaccgatgg
    16921 cgagcaggat cgcgctctga tgcaggaagc tctgctggcc gttgtccgta tgcttaaccc
    16981 gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga
    17041 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
    17101 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ccggtggacg caacggaaga
    17161 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
    17221 tgtacgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
    17281 ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc
    17341 gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa ggtcatgatc
    17401 ctggactcag gcgatccgaa cgggccatta agccgtgcgg tgcgtaacca gttacgtctg
    17461 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cttgcgtttg
    17521 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
    17581 tatcagatga tcatgacggt taatgcgacc gtgttgatcc ccggccgtga tatctacccg
    17641 attagcgcca aagtcttccg ttcgttcttc gataacccgc aaatggcgtt agcgaaagat
    17701 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
    17761 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
    17821 acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg atgattcggt
    17881 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
    17941 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg
    18001 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
    18061 tcttttcgtt atgccaggcc atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
    18121 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
    18181 tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatt
    18241 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
    18301 aggctcagct tccccgctgg gttgctgcgc gcgcaaaaca gctcaattta gaactggatg
    18361 acgcggcgaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
    18421 cactggagcg tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac
    18481 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
    18541 gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg tctggaaggc agcgagccgg
    18601 ttattttgtt gcgcacacta caacgtgaac tgttgttact ggttaacctg aaacgccagt
    18661 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
    18721 gcatgatggg cgaggcgtta aatcgcttaa gccagccgca gttacgtcag gctgtgcaac
    18781 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
    18841 tggaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
    18901 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
    18961 cttaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aataatccct
    19021 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
    19081 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
    19141 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca agggccggac
    19201 gtgccgctgg cgtttattat tggtcaggat tcattgctga cctttccgac ctggtacgaa
    19261 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
    19321 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
    19381 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacaccgtg gtttaacatc
    19441 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
    19501 gaaccggtat tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
    19561 agggcatgat gcgactgtgg ttaattcgtc atggcgaaac acaagcgaat gtcgatggtc
    19621 tgtacagcgg tcatgcgccc acacccctga ccgcacgcgg tattgagcaa gcgcaaaatc
    19681 tgcatacact gctacatgat gtttcctttg acctggtttt atgcagtgaa ctggaacggg
    19741 cacagcatac cgcgcgactg gttctcagtg accgccagct ccccgttcat atcatccctg
    19801 aactcaacga aatgtttttt ggtgactggg agatgcgaca tcatcgcgac ctcatgcaag
    19861 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc cccacgaacg
    19921 gtgaaggatt tcaggcattt tcacaacgtg tggaacgctt tatcgcaagg cttagtgaat
    19981 atcagcacta tcagaatatt ttaatcttca gccatcaggg cgtactgagt ctgttaattg
    20041 ctcgtttaat tggcatgcct gccgagtcta tgtggcattt tcgcgttgac caggggtgtt
    20101 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
    20161 tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
    20221 cagccccggg caggctgata ttctccgcag ccagactttt tccgccagac acgactttgt
    20281 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
    20341 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
    20401 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
    20461 tattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
    20521 caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
    20581 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
    20641 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
    20701 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
    20761 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
    20821 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
    20881 ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
    20941 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
    21001 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta
    21061 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg
    21121 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
    21181 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt
    21241 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
    21301 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
    21361 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca gactcgctct
    21421 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
    21481 aatggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat gccagagaaa
    21541 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac
    21601 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattccggtg
    21661 aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
    21721 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
    21781 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
    21841 aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat
    21901 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
    21961 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
    22021 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt
    22081 gtcaccgatc cgcgtacagg tggggtactg gcgctggttt ccacgcctag ttatgaccca
    22141 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgttgaa cgacccgaat
    22201 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc
    22261 tatgtggcgg tttcggcatt gagcgccggg gtgatcacgc gcaatacgac gctgtttgac
    22321 ccaggctggt ggcaactgcc aggttcggaa aaacgttatc gtgactggaa aaaatggggc
    22381 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
    22441 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
    22501 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
    22561 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggt
    22621 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
    22681 gacggtatcg tgaaggttcc tcatttgctg atgagcaccg ccgaagacgg caaacaggtg
    22741 ccatgggtac agccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
    22801 gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca taaatacttt
    22861 gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt cggtctgaaa
    22921 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    22981 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggt
    23041 ggtgcgggtc cggcggttgg tacactgatg cgccagatcc tcgaccacat tatgctgggt
    23101 gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa
    23161 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    23221 tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    23281 gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctggtcatca
    23341 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    23401 tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggtgctc
    23461 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    23521 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca
    23581 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    23641 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    23701 gctggcgtct gattggcgtc gcagtagtgc tggtggcggc gttcattccg attctgtggt
    23761 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac ccggaatcag
    23821 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    23881 tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc cccgaacgcc
    23941 ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    24001 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    24061 ccacctttgg tcgcgtcatg gctggtggct taatgctgat attattcgtt tatgtcttcg
    24121 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    24181 gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    24241 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    24301 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    24361 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    24421 gcggacccgt gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    24481 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    24541 tatgatgccg aaccaggcag taacctgacg gcctctggcg aagctttcga tccgacacag
    24601 ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    24661 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt
    24721 atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    24781 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    24841 acagtcgcca aacagactta cgccctgcct gcacctcccg atttaagcgg tggcgcggga
    24901 acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa
    24961 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc accaacgacc
    25021 ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca
    25081 gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    25141 agcgccagcg gcaactttat ggtgcaagtc ggggccgtta gcgatcaggc tcgtgcgcaa
    25201 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    25261 gcggtctggc ggatccagct tggccccttc gccagcaaag ccgaagccag taccttgcag
    25321 caacgtttgc aaaccgaagc ccaattacag tcatttatta ccaccgcgca gtagcgtaaa
    25381 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    25441 atgcctgccg gtagtgcatt tgctatagta gggcactttt tttaattcca tcacggatgt
    25501 cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc tggcgctcac
    25561 cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga atatcaaaac
    25621 tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgcttg actataactc
    25681 cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa
    25741 aatgatgacc agttacgtta tcggccaagc aatgaaagcc ggtaaattta aagaaactga
    25801 tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta aaggttcttc
    25861 gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc gcggtattaa
    25921 cctgcaatcg ggtaacgatg cttgtgtcgc catggctgat tttgccgctg gtagccagga
    25981 cgcttttgtt ggcttgatga acagctacgt taacgccctg ggcctgaaaa acacccactt
    26041 ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag atatggcgct
    26101 gatcggccag gcattgatcc gtgacgtacc gaatgaatac tcgatctata aagaaaaaga
    26161 atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg ataacagcct
    26221 gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc ttgttgcttc
    26281 tgcgactgaa ggccagatgc gcttgatttc tgcggtgatg ggcggacgta cttttaaagg
    26341 ccgtgaagcc gaaagtaaaa aactgctaac ctggggcttc cgtttcttcg aaaccgttaa
    26401 cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg attctgatcg
    26461 cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccgcgtggtc gcatgaaaga
    26521 tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc aaaagaatca
    26581 ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgagcaac gcccgctggt
    26641 tgtgttgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt acattaaatt
    26701 aatgttccat cactggtttg gttaaaaatt gaacacttga aagtgtaatt tccgtcccca
    26761 tatactaagc atcagtaaaa aaactcccgc cttctggcgg gagttgctat ttaattacgt
    26821 tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc tactcctttt
    26881 acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt tgaagtggta
    26941 cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa aggcaactac
    27001 cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac actgtatgaa
    27061 gaactgggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc gttacccggc
    27121 tcctggtcgg gtaactcccc tctcctcgct gtgatatact tttcccccac ttttactcat
    27181 tctccacgga gatgccgttt tgtatcagga taaaattctt gtccgccagc tcggtcttca
    27241 gccttacgag ccaatctccc aggctatgca tgaattcacc gatacccgcg atgatagtac
    27301 ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaaggtc aggcaggaaa
    27361 agcggagcac attttaatgc cgggtgatat tccggtgatc cagagcgatc gcggtgggca
    27421 ggtgacttat cacgggccgg ggcaacaggt gatgtatgtg ttgcttaacc tgaaacgccg
    27481 taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga ataccctggc
    27541 tgaactgggt atagaagcgc atcctcgggc tgacgcgcca ggtgtctatg ttggggagaa
    27601 gaaaatttgc tcactgggtt tacgtattcg acgcggttgt tcattccacg gtctggcatt
    27661 aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt atgccggaat
    27721 ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata ttgctccacg
    27781 tttactggaa aatattttag cgctactaaa caatccggac ttcgaatata ttaccgctta
    27841 attccataca tcaatggccc aatttacact gggtcattac tcataatacc tgctaatgct
    27901 ttacatctcc ttcgcgtttg tatattttct ttatcacttg cagaaatgcg gtgccacgcc
    27961 ttgctccgta actcacccca ttgattaacc tattgaaata gttgattatg gtgagcaaaa
    28021 gcgttccgtt ccaattagac ataagattat tgtaattttt taaatattat ttttccatga
    28081 ataaaattgg agtaagtgcg tggatagtaa taatcaaatt gaaccctgtt taagcagaaa
    28141 gtcgtcagaa ggtaaaccac aaatatttac aaccttacga aatattgatc ttaaccttct
    28201 gactattttt gaagctgtat atgtacataa agggatcgtt aatgcagcga aagtgcttaa
    28261 tctgaccccc tcggcaatca gtcagtctat tcagaaactg cgcgttatat tccctgaccc
    28321 attgtttatt cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc atctacatga
    28381 gtatatcagt cagggccttg agtccattct tggcgcgctg gatatcgaag gaagctatga
    28441 taagcaacga acgataacta ttgcgaccac tccctcggtc ggagccctgg tccttcctgt
    28501 catctatcgg gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc cgcccatcag
    28561 cgacgcggaa aaccaactca gtcagtttca aaccgatctc atcatcgata acatgttttg
    28621 caccaatcgt acggtgcaac atcatgttct gttcaccgac aatatggtgt taatttgccg
    28681 cgagggaaat ccactactct ctttagaaga tgacagagag actatcgaca acgctgcgca
    28741 tgtacttctg ttaccggaag agcaaaattt cagcggtctg cggcagagag ttcaagaaat
    28801 gtttccggac cggcaaatca atttcaccag ctacaacatt ttgacaatcg ctgcactggt
    28861 tgccaacagt gacatgttag cgattattcc cagccgtttt tataacctgt ttagccgctg
    28921 ctggccgctg gaaaaactgc cttttccgtc cttaaatgag gagcaaatag atttctctat
    28981 ccactacaac aaattcagcc tgcgtgatcc catcctgcac ggagtaatag atgttatccg
    29041 taatgcattt tgaaaaagta ttgttacagc cagatcacag cgcagaacga taaaatggca
    29101 caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg tccgtttcaa
    29161 tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc tattgaattg
    29221 aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga
    29281 tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg
    29341 tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag
    29401 cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg
    29461 tctgcgagga agcctcctgc cctaacctgg cggaatgctt caaccacggc acagcaacgt
    29521 ttatgatcct cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc
    29581 gcccggtagc tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg
    29641 cgctgcgtta tgtggttatc acctccgttg accgtgatga cctgcgcgat ggcggtgccc
    29701 agcactttgc ggattgcatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa
    29761 ctctggtgcc ggatttccgt ggtcgtatgg atcgtgctct ggatattctg actgcaacgc
    29821 caccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgt caggtacggc
    29881 ctggtgcaga ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg
    29941 aaatcccgac caagtctggt ctgatggtgg gactgggtga aaccaatgaa gaaattattg
    30001 aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg caatatttgc
    30061 agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa
    30121 tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgct
    30181 cttcttacca cgccgatttg caggcgaaag ggatggaagt taagtaatcc tgtaataatt
    30241 acttacatct gtctgataaa aaacccgctt tttgaagcgg gttttttgta tcgaacaaga
    30301 tattgaggga gcgtcctgct cgccacgtca ctctttatga gagagctttt cagcctgaag
    30361 atcaacgtct gcgccttttt tcgccgcagc atcatcatca ttcatcgcct tcttgaaccc
    30421 tttaatggcc gctccaaggt ctccgcccag cgtacgtaac ttcttagtcc caaacagcag
    30481 aacgaccagc gccgcaacta ccagcagttt ggtaatacta atctcaccca tagatacctt
    30541 cttgacatat aaccggcaac agaacgcgcc ttaaaaacca gagaatatta acggtcattc
    30601 gacgcgcgca cacaatagca atctgtaaca aggtgaatca agcgccgagt aaaaaaacat
    30661 cataataatt gcggcggcgc aaagcgacgg ttgtttaaga cgggcagttg cgcgcgcacc
    30721 tgacgcacac gttcgggcgt cacttccgcc ataatgagtg caggcatttc tgacgctgcc
    30781 gcaatggtga ctccaaaggg atctataatc cggctttgac cgatattttt gttcccgcac
    30841 tcccccgccg ccaccatata acaggtggta tccagcacac gagcggcaag caacgttgac
    30901 cagtgatgct ctttgagcgg cccgcgaacc caggcggcag gaagtaccag gatttcagct
    30961 ccctgtaatg cctgtgccag cgccagctct ggaaagcgta agtcataaca ggtcatcaga
    31021 ccgaccttca tcccctccac ctcgagtaac ggagcgattt cattaccagc atcaacacgg
    31081 cgtgattcct gaatggcaaa tgcatcatag agatgcaatt tggcataacg ggcgacgatg
    31141 tttcctgcct gtagcgccac cagcatattc catgcgcgcc ccggcgttga aggaacatga
    31201 atcgtcagaa ttgtcgtcat catgttacgt ttactttctc gccgtaaacg tccgaggaat
    31261 tcgccttcca gcagctgtgc tgatttaacc gatagatctg catcatgatc atcgcgcgcc
    31321 agcaatgctt ccggcaggac gaacagcgat acgtcgtttt ccgccgcctg cgccatcaac
    31381 gaggcacaaa tctcagcgtt cttttcccac acagatgtaa cagcaaactg tcctgcggca
    31441 accagcattg ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt
    31501 acacttatca ctcatacaaa tcaaatagca ggattttgca gtgttacaac ttcttttagc
    31561 agtttttatt ggcggtggta cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa
    31621 cccgctgcat caggcgattc cgttggggac gctggcagca aacctgattg gggcattcat
    31681 cataggaatg ggattcgcct ggttcagcag gatgacgaac attgatccag tgtggaaagt
    31741 attaatcacc accggatttt gtggcggtct aacaaccttc tcaacatttt cggcagaagt
    31801 ggtgtttttg ttacaagagg gccgctttgg ctgggcgtta ctgaacgttt tcgtcaacct
    31861 tctggggtct tttgccatga cagcactggc tttctggctg ttttcggcct caaccgcaca
    31921 ctaaagacca aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt
    31981 acagagcgat tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta
    32041 cgcgctgacc ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga
    32101 acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc
    32161 acttaacgtt acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa
    32221 atgctgtgtc ggttaccagt acaccaattg tggtacgctt tgtccagtgc aactttacta
    32281 aaaagtgata aaagtcgcgt tatttttgat gctacttatt ttagttattg attttaaaga
    32341 agttactaaa acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc
    32401 cattgttgta ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg
    32461 aggccaatgg cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc
    32521 cagcggtgaa tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt
    32581 cccatccgta tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat
    32641 acaggccatg ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg
    32701 gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga
    32761 tggcaggaat atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc
    32821 taaacgttgt catccactca tctgcgttag caaaaacttt accaacgctg attaactgaa
    32881 taaaaacaaa ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat
    32941 ttatcaaaaa tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca
    33001 acataaagaa taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt
    33061 ccgcgcatcg attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa
    33121 accaagtctt ttttgatgac aaaatgcaat caaggaaaaa ttattttatt ttttaacgag
    33181 atacaacaat catcttaacg aagcatataa tatatatcgt caacctatgc tcatattaga
    33241 ttattttttg agcgcacagc cgcagctaac accattgcaa ttaacaaatt tgcatcaatc
    33301 caccatcaat ttgcacacat tattatgtga taattgccaa ccgctaaata tgcgttttgt
    33361 tatctatgta taaaaacagc aacttcaatg tcttaatggc agtttttctt gattttaatc
    33421 agcattcatc gccaatttat tgggcatatt ttttccttaa gctttaggaa ttttttatta
    33481 tttactttgg ggcctggaga caggaaaaat tatgctgaca ttcattgagc tccttattgg
    33541 ggttgtggtt attgtgggtg tagctcgcta catcattaaa gggtattccg ccactggtgt
    33601 gttatttgtc ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt
    33661 accgtccagc caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat
    33721 attactaatg agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggatttgc
    33781 cgcttacatg acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt
    33841 gcagtatatt aactcccctt acctgctgat gattgccgcc tattttgtcg cctgtctgat
    33901 gtctctggcc gtctcttccg caaccggtct gggtgttttg ctgatggcaa ccctatttcc
    33961 ggtgatggta aacgttggta tcagtcgtgg cgcagctgct gccatttgtg cctccccggc
    34021 ggcgattatt ctcgcaccga cttcagggga tgtggtgctg gcggcgcaag cttccgaaat
    34081 gtcgctgatt gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg
    34141 catggcgatc gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca
    34201 tgaaatgtta gatgtcagtg aaatcaccac cactgctcct gcgttttatg ccattttgcc
    34261 gttcacgccg atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat
    34321 catcactatt ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt
    34381 taatacccag aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt
    34441 tgctaacgtg gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat
    34501 cggctttatt caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct
    34561 gatgctggta ttggtgattc tgacaatgct ggcggcagtc acgaccggtt caggcaatgc
    34621 gccgttttat gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc
    34681 ggcgtatttg actatcccga tgctgcaggc gtcaaacctt ggccgtaccc tttcgcccgt
    34741 ttctggcgta gtcgttgcgg ttgccgggat ggcgaagatc tcgccgtttg aagtcgtaaa
    34801 acgcacctcg gtaccggtgc ttgttggttt ggtgattgtt atcgttgcta cagagctgat
    34861 ggtgccagga acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt
    34921 cctccggctt tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg
    34981 tgaacaattt cagcaataat cagatggcgg ctggcgcaat attcaagata acgtctggca
    35041 gtggtgcggc tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc
    35101 tctttaaaca gttttcgcac cgcgtttagc gtcaagggat caatgccggt cggcagttcg
    35161 tctttaggtt caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg
    35221 ctatcaatac tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagc
    35281 cgttcatagg caatgggctt aatgagataa tcaaatacac cacaacgtac ggcttcagac
    35341 accgtttcca tatcgctggc tgcagtggta aacaccacgt cgccgggata atgcgcctgc
    35401 accagttcat gcaataaatt aatccctctg ccgtcaggaa gatagttatc gagcaagatt
    35461 agccccggct taaaacgctc gatcatcatt cgggcctgcg ccaggtttcc cgccagtaat
    35521 atctgactga atccgggaat gtgacgaata tattccgcat gcatctctgc cagcggcgtt
    35581 tcgtcctcaa cgatcaatag ggttaatgga gctgtcatta ggtttcactt tcggaatata
    35641 gattgaaaat aaggtaccgc agggatcatt atcttcgaga gtgataacac caccgcagcg
    35701 cgttacgtag ctggcaatca agtacaaccc aatgccatgt tcaccgggct cgtcagcacg
    35761 cgtactgacc ccctgctcaa atattttgtc tcgtagagac tctggaacgc cgcagccctg
    35821 atcggcgact tcaatcacca catcatcgcc ttcatcgctg aggaataatt caacgatctt
    35881 gtttccttca tcgctacgca ggctggcttc gaaggcgtta tcaagtaaat tgcccacaat
    35941 ggctgcaaac tcggtgctat ccagtcctgg tggcagttgc gaaagctggc taccggggac
    36001 aatgatcatt tttagcccca gttcccgggc gcgctgcact ttaccaaaaa gcagccccgc
    36061 cacctggcga tcggcaaacg cctcgcgcag gctgtcaata agctgttgct gggcctgaga
    36121 ctccccctgc accatcgcca gcacgcgatc atactctttc atctgcaaca gaccattgag
    36181 cgtcgacatc caattgagat gctcgtgtcg caatgtacga aggctctcaa cgtattgttt
    36241 tatttgcgtc agttgcgcat tgagggtgga aatttcgtct ttactgcgaa agctgataat
    36301 ggcccccagc aaatcatcac ctgaacgaat agcttcccga ttggcaataa cgctcagacc
    36361 gttaaagtta gccaccacat cctgacgttt ttcatcaatc tgttcggtaa agaaatcggc
    36421 gggcctgacc acttcagcaa tggttttacc caaccattgc cgtccggggg aactcagccc
    36481 cagcatcttt cttgcgttac gattgatggc ggtaatgtaa ccgtgcggat ccaccgcaat
    36541 cagcccttca taaaccgaac taaacagcgc ctcttgctga cggaccacgc gtgcgatttg
    36601 ctttggctcc atgcccatca tctgccgacg gatatgcgcg gccaggaacc acgacagcaa
    36661 catcagaatc cctaacagca cgacaaacac acctgccatc ggtaataaaa actcagcccg
    36721 ccagctatcg attttactca ccaggtagcc aatcgacacc acgccgatga cttttccatc
    36781 gtcatcaaag attggcgttt tggcgcgcat cgccatcccc attgaccctt tcccggtaat
    36841 gaagtagctc tcccctttct ccagcgcgcc ctgtttggtg aactgcatag gataaccaat
    36901 tttctccgga ttaggatggt aaaggcggat cgagtgccgg tccccaatca ccacataatc
    36961 aaaatcggta tctctttgta atttgttagc gatggtcgcc agccgtttgt agtcacgcgt
    37021 tttcaccgca gagatgacac tgtcattgga ggcaataatt ttcgcctgat tcattgccat
    37081 gtcgcgtaca tgaagcgtta aatagtcctc aaaactggcc gtaaaatatt gcgccagggc
    37141 tgcaatgaca aatattgaga acaccagaat cagcaaaaag atacgcagcg gaaatgccag
    37201 tctttggaaa aatgcaaact gtttattctc gttaagctgc aacattgttt tccttgcggg
    37261 ttgtcccgag cctctttgac ggatgtaatt aaaatgttga atcaaaatac atagatatta
    37321 attatgatcg tggcaaattt tattcattaa atcaattaaa tcacttaaac ctgcgagtca
    37381 tttcgtaatt acattaatta aaaaacttaa aaccattaaa taaataaaaa accactaact
    37441 ctatgtgaaa taaatcaaaa tttcacgccg aaatactcct taggatgtat agcgaaagga
    37501 gaaaaagata tacctcgatc acccaccttt ctcccaagtg aaaataaaaa gttatcagtt
    37561 tgcaacattg aacaacattc gttgcaaatc gataacaaca tgcaccttca ggatactatt
    37621 tattatgttc ggcaatgata ttttcacccg cgtaaaacgt tcagaaaata aaaaaatggc
    37681 ggaaatcgcc caattcctgc atgaaaatga tttgagcgtt gacaccacag tcgaagtatt
    37741 tattaccgta acccgcgatg aaaagcttat cgcgtgcggt ggaattgccg gaaatattat
    37801 taaatgcgtt gctatcagtg aatccgttcg cggtgaagga ctggcgctga cattagccac
    37861 tgaactgata aacctcgcct atgagcggca cagcacgcat ctgtttattt ataccaaaac
    37921 cgaatacgag gcgctgttcc gccagtgcgg tttttccacg ctgaccagcg tacccggcgt
    37981 gatggtgctg atggaaaaca gcgccacgcg actgaaacgc tatgccgaat cgctgaaaaa
    38041 atttcgtcat ccagggaaca agattggctg cattgtgatg aacgccaatc cctttacgaa
    38101 tggtcaccgt tatctgattc aacaggctgc ggcacagtgc gactggttgc atctgttttt
    38161 agtcaaagaa gattcttcac gcttccccta tgaagaccgg ctggatctgg tgttaaaagg
    38221 caccgccgat attccacgcc tgactgtgca tcgcggctcc gaatacatca tctcccgcgc
    38281 tacgttccct tgctacttca ttaaagaaca gagcgtcatt aaccattgtt acaccgaaat
    38341 tgatctgaag attttccgtc agtacctcgc tcccgcactg ggtgtaactc accgctttgt
    38401 cggtactgaa cccttttgtc gcgttaccgc ccagtacaac caggatatgc gctactggct
    38461 ggaaacgccg actatctccg caccgcccat cgaactggtt gaaattgagc ggctgcgtta
    38521 ccaggagatg ccgatatccg cttcccgggt acgtcaactg ctggcgaaaa acgatctcac
    38581 ggctatcgcg ccgctggtcc ctgcagtcac gctgcattat ttgcagaacc tgcttgagca
    38641 ctcccgccag gacgcggcag ctcgtcaaaa gacccccgca tgagaaacag gtgaaaaatg
    38701 aaaataaacc agcccgccgt tgcaggcacc cttgagtctg gggatgtgat gatacgcatc
    38761 gccccactcg atacgcagga tatcgacctg caaatcaaca gcagcgttga gaaacagttt
    38821 ggcgatgcaa ttcgcaccac cattctggac gttctcgccc gctacaacgt gcgcggcgta
    38881 cagctgaatg tcgatgacaa aggcgcactg gactgcattt tacgtgcacg actggaagcc
    38941 ctgctggcac gcgccagcgg tatcccggct ctgccatggg aggattgcca atgatttccg
    39001 cttcgctgca acaacgtaaa actcgcaccc gccgcagcat gttgtttgtg cctggtgcca
    39061 atgccgcgat ggtcagcaac tccttcatct acccggctga tgccctgatg tttgacctcg
    39121 aagactccgt agcattgcgt gaaaaagaca ccgcccgccg catggtttac cacgcgctgc
    39181 aacatccgct gtatcgcgat attgaaacca ttgtgcgtgt caacgcgctg gattccgaat
    39241 ggggtgtcaa cgacctggaa gccgtcgttc gcggtggtgc ggacgttgtg cgtctgccga
    39301 aaaccgatac cgctcaggat gttctggata tcgaaaaaga gatcctgcgt atcgaaaaag
    39361 cctgtggtcg tgaacccggc agcaccggcc tgctggcggc gattgaatct ccgctgggca
    39421 ttacccgcgc agtggaaatc gctcacgctt ccgagcgttt gatcggtatc gccctcggtg
    39481 cagaagacta tgtgcgcaac ctgcgtacag aacgctcccc ggaaggaact gaactgctgt
    39541 tcgctcgctg ctccattttg caggccgcgc gctctgcggg tattcaggcg ttcgataccg
    39601 tctattccga cgctaacaac gaagctggat tcctgcaaga agccgcccac atcaaacagc
    39661 tgggctttga cggcaaatcg ctgatcaacc cgcgtcagat tgatctgctg cacaacctct
    39721 acgcaccgac ccagaaagaa gtggatcacg cccgccgcgt cgtagaagcc gctgaagccg
    39781 ccgctcgcga aggcctcggc gtggtttccc tgaacggcaa gatggtggac ggtccggtta
    39841 tcgatcgcgc ccgtctggtg ctctcccgtg cagaactttc cggcatccgc gaagaataag
    39901 gcaatcaaaa tgacgcagaa aattgaacaa tctcaacgac aagaacgggt agcggcctgg
    39961 aatcgtcgcg ctgaatgcga tcttgccgct ttccagaact caccaaagca aacctaccag
    40021 gctgaaaaag cgcgcgatcg caaactgtgc gccaacctgg aagaagcgat tcgtcgctct
    40081 ggtttacagg acggcatgac ggtttccttc catcacgctt tccgtggcgg tgacctgacc
    40141 gtcaatatgg tgatggacgt catcgcgaag atgggcttta aaaacctgac cctggcgtcc
    40201 agctccctga gtgattgcca tgcgccgctg gtagaacaca ttcgccaggg cgtggttacc
    40261 cgcatttata cctccggcct gcgtggtcca ctggcggaag agatctcccg tggtctgctg
    40321 gcggaaccgg tgcagatcca ctctcacggc ggacgtgtgc atctggtaca gagcggcgaa
    40381 ctgaatatcg acgtggcttt cctcggcgtc ccgtcctgtg atgaattcgg taatgccaac
    40441 ggctacaccg gtaaagcctg ctgcggctcc ctcggctatg caatggttga tgccgacaac
    40501 gcaaaacagg ttgtgatgct taccgaagaa ctgctgcctt atccgcataa tccggcaagc
    40561 attgagcaag atcaggttga tctgatcgtc aaagttgatc gcgttggcga tgctgcaaaa
    40621 atcggcgctg gcgctacccg tatgaccact aacccgcgcg aactgcttat tgcccgtagc
    40681 gctgcggatg tgattgtcaa ctctggctac ttcaaagaag gtttctccat gcaaaccggc
    40741 accggcggcg catcgctggc ggtaacccgt ttcctggaag acaaaatgcg tagccgcgat
    40801 attcgcgccg acttcgccct tggcggtatt accgcgacga tggttgacct gcacgaaaaa
    40861 ggtctgatcc gcaaactgct ggatgtgcag agctttgaca gccatgctgc gcaatcgctg
    40921 gcccgtaacc ccaatcacat cgaaatcagc gccaaccagt acgctaactg gggttcgaaa
    40981 ggcgcatcgg ttgatcgtct cgacgtggtg gtactgagcg cgctggaaat tgacacccag
    41041 ttcaacgtta acgtgctgac cggctctgac ggcgtactgc gtggtgcttc cggtggtcac
    41101 tgcgataccg caattgcctc tgcgctttcc atcatcgtcg cgccgctggt acgcggtcgt
    41161 attccgactc tggtggataa cgtgctgacc tgcatcaccc cgggctccag tgtcgatatt
    41221 ctggtcacag accacggtat cgcagttaac ccggcacgtc cggaactggc agaacgtctg
    41281 caggaagcgg gcattaaagt ggtttccatt gagtggctgc gcgaacgtgc gcgtctgctg
    41341 accggtgaac cacagccgat tgaattcacc gaccgcgtcg ttgccgttgt gcgttaccgc
    41401 gatggctcgg tgatcgatgt tgtgcatcag gtgaaggaat aagccatgca cctgcttcct
    41461 gaactcgcca gccaccatgc ggtatcaatt cccgagctgc tcgtcagccg ggatgaaagg
    41521 caagcacggc aacacgtctg gctcaagcgc catcctgttc c
//
LOCUS       DACAPB010000015        40927 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.015, whole genome shotgun sequence.
ACCESSION   DACAPB010000015 DACAPB010000000
VERSION     DACAPB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.015"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..485)
                     /locus_tag="IT904_000734"
     CDS             complement(<1..485)
                     /locus_tag="IT904_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190562.1"
                     /translation="MLPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNININCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     V"
     gene            complement(507..793)
                     /locus_tag="IT904_000735"
                     /pseudo
     CDS             complement(507..793)
                     /locus_tag="IT904_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(803..1045)
                     /locus_tag="IT904_000736"
     CDS             complement(803..1045)
                     /locus_tag="IT904_000736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190563.1"
                     /translation="MKGFPIAHIFHPSIPPMYAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRI"
     gene            complement(1301..1645)
                     /gene="tdcR"
                     /locus_tag="IT904_000737"
     CDS             complement(1301..1645)
                     /gene="tdcR"
                     /locus_tag="IT904_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAP3190564.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSVINNAACHIFSRYLLENKHLFYQYFKISNTCIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            1833..2771
                     /gene="tdcA"
                     /locus_tag="IT904_000738"
     CDS             1833..2771
                     /gene="tdcA"
                     /locus_tag="IT904_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAP3190565.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            2870..3859
                     /gene="tdcB"
                     /locus_tag="IT904_000739"
     CDS             2870..3859
                     /gene="tdcB"
                     /locus_tag="IT904_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAP3190566.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            3881..5212
                     /gene="tdcC"
                     /locus_tag="IT904_000740"
     CDS             3881..5212
                     /gene="tdcC"
                     /locus_tag="IT904_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAP3190567.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            5238..6446
                     /gene="tdcD"
                     /locus_tag="IT904_000741"
     CDS             5238..6446
                     /gene="tdcD"
                     /locus_tag="IT904_000741"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAP3190568.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGLEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            6480..8774
                     /gene="tdcE"
                     /locus_tag="IT904_000742"
     CDS             6480..8774
                     /gene="tdcE"
                     /locus_tag="IT904_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAP3190569.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            8788..9177
                     /locus_tag="IT904_000743"
     CDS             8788..9177
                     /locus_tag="IT904_000743"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAP3190570.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            9249..10613
                     /locus_tag="IT904_000744"
     CDS             9249..10613
                     /locus_tag="IT904_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase TdcG"
                     /protein_id="HAP3190571.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            10888..12219
                     /locus_tag="IT904_000745"
     CDS             10888..12219
                     /locus_tag="IT904_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP3190572.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            12247..13557
                     /locus_tag="IT904_000746"
     CDS             12247..13557
                     /locus_tag="IT904_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAP3190573.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(13691..13855)
                     /locus_tag="IT904_000747"
     CDS             complement(13691..13855)
                     /locus_tag="IT904_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190574.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13878..14579)
                     /locus_tag="IT904_000748"
     CDS             complement(13878..14579)
                     /locus_tag="IT904_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP3190575.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            14684..15580
                     /locus_tag="IT904_000749"
     CDS             14684..15580
                     /locus_tag="IT904_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3190576.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            complement(15631..15987)
                     /locus_tag="IT904_000750"
     CDS             complement(15631..15987)
                     /locus_tag="IT904_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP3190577.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(16229..16594)
                     /locus_tag="IT904_000751"
     CDS             complement(16229..16594)
                     /locus_tag="IT904_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP3190578.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(16877..17863)
                     /locus_tag="IT904_000752"
     CDS             complement(16877..17863)
                     /locus_tag="IT904_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP3190579.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKYRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(17933..18415)
                     /locus_tag="IT904_000753"
     CDS             complement(17933..18415)
                     /locus_tag="IT904_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAP3190580.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVSGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            complement(18511..18810)
                     /locus_tag="IT904_000754"
     CDS             complement(18511..18810)
                     /locus_tag="IT904_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190581.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLETTGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(18800..19204)
                     /locus_tag="IT904_000755"
     CDS             complement(18800..19204)
                     /locus_tag="IT904_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190582.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(19207..19512)
                     /locus_tag="IT904_000756"
     CDS             complement(19207..19512)
                     /locus_tag="IT904_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAP3190583.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(19550..19918)
                     /locus_tag="IT904_000757"
     CDS             complement(19550..19918)
                     /locus_tag="IT904_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAP3190584.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(20065..20448)
                     /gene="mzrA"
                     /locus_tag="IT904_000758"
     CDS             complement(20065..20448)
                     /gene="mzrA"
                     /locus_tag="IT904_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAP3190585.1"
                     /translation="MQMPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(20452..21114)
                     /locus_tag="IT904_000759"
     CDS             complement(20452..21114)
                     /locus_tag="IT904_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP3190586.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(21459..22235)
                     /gene="exuR"
                     /locus_tag="IT904_000760"
     CDS             complement(21459..22235)
                     /gene="exuR"
                     /locus_tag="IT904_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAP3190587.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(22365..23663)
                     /locus_tag="IT904_000761"
     CDS             complement(22365..23663)
                     /locus_tag="IT904_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3190588.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            24146..25558
                     /gene="uxaC"
                     /locus_tag="IT904_000762"
     CDS             24146..25558
                     /gene="uxaC"
                     /locus_tag="IT904_000762"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP3190589.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            25573..27060
                     /locus_tag="IT904_000763"
     CDS             25573..27060
                     /locus_tag="IT904_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP3190590.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            27143..27694
                     /locus_tag="IT904_000764"
     CDS             27143..27694
                     /locus_tag="IT904_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAP3190591.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(27699..28943)
                     /gene="sstT"
                     /locus_tag="IT904_000765"
     CDS             complement(27699..28943)
                     /gene="sstT"
                     /locus_tag="IT904_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP3190592.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDGRLANSALRN"
     gene            complement(29342..30307)
                     /gene="alx"
                     /locus_tag="IT904_000766"
     CDS             complement(29342..30307)
                     /gene="alx"
                     /locus_tag="IT904_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAP3190593.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(30590..31576)
                     /locus_tag="IT904_000767"
     CDS             complement(30590..31576)
                     /locus_tag="IT904_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP3190594.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKINVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(31655..32347)
                     /locus_tag="IT904_000768"
     CDS             complement(31655..32347)
                     /locus_tag="IT904_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAP3190595.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATILIADRVMVNAS
                     KQLTWSNVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAQK"
     gene            complement(32424..32927)
                     /locus_tag="IT904_000769"
     CDS             complement(32424..32927)
                     /locus_tag="IT904_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP3190596.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKVEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            33012..34148
                     /gene="rlmG"
                     /locus_tag="IT904_000770"
     CDS             33012..34148
                     /gene="rlmG"
                     /locus_tag="IT904_000770"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAP3190597.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPQLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            34432..34746
                     /locus_tag="IT904_000771"
     CDS             34432..34746
                     /locus_tag="IT904_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAP3190598.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            34743..35159
                     /gene="higA"
                     /locus_tag="IT904_000772"
     CDS             34743..35159
                     /gene="higA"
                     /locus_tag="IT904_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAP3190599.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(35204..37222)
                     /locus_tag="IT904_000773"
     CDS             complement(35204..37222)
                     /locus_tag="IT904_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAP3190600.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(37648..39999)
                     /gene="ygjK"
                     /locus_tag="IT904_000774"
     CDS             complement(37648..39999)
                     /gene="ygjK"
                     /locus_tag="IT904_000774"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP3190601.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(40016..>40927)
                     /gene="ygjJ"
                     /locus_tag="IT904_000775"
     CDS             complement(40016..>40927)
                     /gene="ygjJ"
                     /locus_tag="IT904_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HAP3190602.1"
                     /translation="LGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWT
                     VQADHDGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFT
                     PALGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYT
                     YPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDV
                     PGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFAT
                     AFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT        10078 a        10188 c        10556 g        10105 t
ORIGIN      
        1 tcgacattac gtaagtccag agaggataga tcaacgatat ctccagtccc acaactaaaa
       61 attacgttta acctcctgat ttcattgtaa atatctttta atgggcaatt aatattgata
      121 ttacctgtgg atatttcctg gttttttaac ggttcattaa tggtaagttc acaagtattg
      181 tccgatcgtt gaataatttg ggcgttataa cctaaaaaat ccagacgaag agacgagaca
      241 ttataaagaa ttacattttg taagtacgct aattcttttt gaaaatcacc agtgataatg
      301 cattttccac caggttgtaa cgcgttaagt aaagtgacca tttgaataga ggtctgtttt
      361 agtttttcta tacctggtgc tagtgcttcg aggttatttc ctgttgggct ttcaggaagg
      421 tcattcattt ttcgacggac taaatcacta ctaatatcat taccaattga tgaaacaggg
      481 agcattattt tgtcctttat aaataatcaa taaaaatcat tattaaaatg atcgggtgaa
      541 aaaaagagaa tttgccaata atctgaagta atggaggttt tattaaggtg cgtagggggg
      601 gtaagatggc ttctttttgg gtgaactttg cgcaatactt agaaatcgtc tccttaatac
      661 attccagctc ttttttattg acggctgagc aagtacattg ttcaccaaaa ggtgcaaaag
      721 tgcctacatc taatgtttgt atgatataat cgtcatgttc tcctgtagaa atatctataa
      781 tgaatggact catattttcc ggttagattc ggatcatgac agaaggaaca tcgccttgat
      841 agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt atactgtaaa
      901 ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc gtccaataat
      961 caatatttct attgtgattg ttaaccactg catacattgg cggaattgaa gggtgaaaaa
     1021 tatgtgcaat tgggaagcct ttcattgatg cctgccttgt catcatgaca aacaataata
     1081 gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat ttacccggcg
     1141 ttacagtaaa cgaagcaaca ttctgtggcc tacgccgatc aaaagctctg tgaaaatttc
     1201 ccttgttgtc tttgtctgac cttatcgcgg cgatcagcaa ttattcgttt tgtgatcctc
     1261 aacacaaaat gctattcgtc gcctatgagg ggagaagaat ttatggaaaa cgattgcatt
     1321 cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaattgt tcgagatgat
     1381 caatacatgt attcgaaatt ttaaaatatt gataaaataa atgtttgttc tccaacaaat
     1441 agcgtgaaaa aatgtgacat gccgcattat ttatcactga aaatggttta tttataataa
     1501 aatttgagaa cttgctcatt aaatttaact caaattttgc ctggtaatta tccggtaatt
     1561 gcttgaaata tggtctcagc cccttttttg tattaaccac ataacgaatg atgttatcgc
     1621 cataaaatat ggttatcccc gtcatttttt tgacaaaaat cagggtttat gctgattttt
     1681 atactttaac ttgttgatat ttaaaggtat ttaattgtaa taacgatact ctggaaagta
     1741 ttgaaagtta atttgtgagt ggtcgcacat atcctgttca tttcattttg atacacttca
     1801 tgccgtcaat gaggtaatta acgtaggtcg ttatgagcac tattcttctt ccgaaaacgc
     1861 agcacctggt agtctttcag gaagtcatta gaagtggttc tatcggctcg gctgcaaaag
     1921 aattagggtt aactcaaccg gccgtcagta aaatcattaa cgatattgaa gattattttg
     1981 gtgtggaatt agtggtgcgg aaaaataccg gtgtaacatt aacacctgcc ggtcaattgt
     2041 tactctcccg ttccgaatcc attacccgtg aaatgaaaaa tatggttaat gagataagcg
     2101 gtatgtcttc tgaggcggtg gtggaagtct catttggttt tccttcattg attggtttta
     2161 cttttatgtc agggatgatc aacaagttca aagaggtgtt cccgaaagcg caggtttcta
     2221 tgtatgaagc gcaactgtct tcgttcttac cggcaatccg cgacggtcga ctggattttg
     2281 cgattggtac gttaagtgca gaaatgaagc ttcaggattt acatgttgag ccgctgttcg
     2341 agtccgagtt tgtgctggta gccagtaagt cccgaacatg caccggcacc accacgctgg
     2401 agtcgttgaa gaacgaacag tgggtgttgc cacaaactaa tatggggtac tacagcgaac
     2461 tgcttactac gttacaaaga aatggcatca gtattgaaaa catcgttaaa accgactcag
     2521 tcgtgacaat ttataatctt gttctcaatg ctgatttctt aactgtaatt ccttgtgata
     2581 tgacgtcacc ttttggttct aatcaattta ttactattcc ggttgaagaa acattacctg
     2641 tggcacaata tgccgcggta tggtcgaaaa attatcgtat taaaaaagca gcatcggttt
     2701 tggtggaatt agccaaagag tattcatctt ataatgggtg tagacgaagg caattaatag
     2761 aagttggtta gttatttgtt tttatttaaa cataaataat ccacctgtct gtcttgcagg
     2821 tgtcggttac ggttacctac atatttaatt caggcgaaga ggttttataa tgcatattac
     2881 atacgatctg ccggttgcta ttgatgacat tattgaagcg aaacaacgac tggctgggcg
     2941 aatttataaa acaggcatgc ctcgctccaa ctattttagt gaacgttgca aaggtgaaat
     3001 attcctgaag tttgaaaata tgcagcgtac gggttcattt aaaattcgtg gcgcatttaa
     3061 taaattaagt tcactgaccg atgcggaaaa acgcaaaggc gtggtggcct gttctgcggg
     3121 caaccatgcg caaggggttt ccctctcctg cgcgatgctg ggtatcgacg gtaaagtggt
     3181 gatgccaaaa ggtgcgccaa aatccaaagt agcggcaacg tgcgactact ccgcagaagt
     3241 cgttctgcat ggtgataact tcaacgacac tatcgctaaa gtgagcgaaa ttgtcgaaat
     3301 ggaaggccgt atttttatcc caccttacga tgatccgaaa gtgattgctg gccagggaac
     3361 gattggtctg gaaattatgg aagatctcta tgatgtcgat aacgtgattg tgccaattgg
     3421 tggtggcggt ttaattgctg gtattgcggt ggcaattaaa tctattaacc cgaccattcg
     3481 tgttattggc gtacagtctg aaaacgttca cggcatggcg gcttctttcc actccggaga
     3541 aataaccacg caccgaacta ccggcaccct ggcggatggt tgtgatgtct cccgcccggg
     3601 taatttaact tacgaaatcg ttcgtgaatt agtcgatgac atcgtgctgg tcagcgaaga
     3661 cgaaatcaga aacagtatga ttgccttaat tcagcgcaat aaagtcgtca ccgaaggcgc
     3721 aggcgctctg gcatgtgctg cattattaag cggtaaatta gaccaatata ttcaaaacag
     3781 aaaaaccgtc agtattattt ccggcggcaa tatcgatctt tctcgcgtct ctcaaatcac
     3841 cggtttcgtt gacgcttaat taattcgttg aggataggat atgagtactt cagatagcat
     3901 tgtatccagc cagacaaaac aatcgtcctg gcgtaaatca gataccacat ggacgttagg
     3961 cttgtttggt acggcaatcg gcgccggggt gctgttcttc cctatccgcg caggttttgg
     4021 cggactgatc ccgattcttc tgatgttggt attggcatac cccatcgcgt tttattgcca
     4081 ccgggcgctg gcgcgtctgt gtctttctgg ctctaaccct tccggcaaca ttacggaaac
     4141 ggtggaagag cattttggta aaactggcgg cgtggttatc acgttcctgt acttcttcgc
     4201 gatttgccca ctgctgtgga tttatggcgt tactattacc aataccttta tgacgttctg
     4261 ggaaaaccag ctcggctttg caccgctgaa tcgcggcttt gtggcgctgt tcctgttgct
     4321 gctgatggct ttcgtcatct ggtttggtaa ggatctgatg gttaaagtga tgagctacct
     4381 ggtatggccg tttatcgcca gcctggtgct gatttctttg tcgctgatcc cttactggaa
     4441 ctctgcagtt atcgaccagg ttgacctcgg ttcgctgtcg ttaaccggtc atgacggtat
     4501 cctgatcact gtctggctgg ggatttccat catggttttc tcctttaact tctcgccaat
     4561 cgtctcttct ttcgtggttt ctaagcgtga agagtatgag aaagacttcg gtcgcgactt
     4621 caccgaacgt aaatgttccc aaatcatttc tcgtgccagc atgctgatgg ttgcagtggt
     4681 gatgttcttt gcctttagct gcctgtttac tctgtctccg gccaacatgg cggaagccaa
     4741 agcgcagaat attccagtgc tttcttatct ggctaaccac tttgcgtcca tgaccggtac
     4801 caaaacaacg ttcgcgatta cactggaata tgcggcttcc atcatcgcac tcgtggctat
     4861 cttcaaatct ttcttcggtc actatctggg aacgctggaa ggtctgaatg gcctggtcct
     4921 gaagtttggt tataaaggcg acaaaactaa agtgtcgctg ggtaaactga acactatcag
     4981 catgatcttc atcatgggct ccacctgggt tgttgcctac gccaacccga acatccttga
     5041 cctgattgaa gccatgggcg caccgattat cgcatccctg ctgtgcctgt tgccgatgta
     5101 tgccatccgt aaagcgccgt ctctggcgaa ataccgtggt cgtctggata acgtgtttgt
     5161 taccgtgatt ggtctgctga ccatcctgaa catcgtatac aaactgtttt aatccgtaac
     5221 tcaggatgag aaaagagatg aatgaatttc cggttgtttt ggttattaac tgtggttcgt
     5281 cttcgattaa gttttccgtg ctcgatgcca gcgactgtga agtattaatg tcaggtattg
     5341 ccgacggtat taactcggaa aatgcattct tatccgtaaa tgggggagag ccagcaccgc
     5401 tggctcacca cagctacgaa ggtgcattga aggcaattgc atttgaactg gaaaaacgga
     5461 atttaaatga cagtgtggcc ttaattggcc accgcatcgc tcacggcggc agtattttta
     5521 ccgagtccgc cattattacc gatgaagtca ttgataatat ccgtcgcgtt tctccactgg
     5581 cacccctgca taattacgcc aatttaagtg gtattgaatc ggcgcagcaa ttatttccgg
     5641 gcgtaactca ggtggcggta tttgatacca gtttccacca gacgatggct ccggaagctt
     5701 atttatacgg cctgccgtgg aaatattatg aagagttagg tgtacgccgt tatggtttcc
     5761 acggcacgtc gcaccgctat gtttcccagc gcgcacattc gctgctgaat ctggcggaag
     5821 atgactccgg cctggttgtg gcgcatcttg gcaatggcgc gtcaatctgc gcggttcgca
     5881 acggtcagag tgttgatacc tcaatgggaa tgacgccgct ggaaggcttg atgatgggta
     5941 cccgcagtgg cgatgtcgac tttggtgcga tgtcctgggt cgccagccaa accaaccaga
     6001 gcctgggtga cctggaacgc gtagtgaata aagagtcggg attattaggt atttccggtc
     6061 tttcttcgga tttacgtgtt ctggaaaaag cctggcatga aggtcacgaa cgcgcgcaac
     6121 tggcaattaa aacctttgtt caccgaattg cccgtcatat tgccggacac gcagcttcat
     6181 tacgtcgcct ggatggaatt atattcaccg gcggaatagg agagaattca agcttaattc
     6241 gtcgtctggt catggaacat ttggctgtat taggcttaga gattgataca gaaatgaata
     6301 atcgctctaa ctcctgtggt gagcgaattg tttccagtga aaatgcgcgt gtcatttgtg
     6361 ccgttattcc gactaacgaa gaaaaaatga ttgctttgga tgccattcat ttaggcaaag
     6421 ttaacgcgcc cgcagaattt gcataattta gttgaagtat tgtagagaga ttatttttca
     6481 tgaaggtaga tattgatacc agcgataagc tgtacgccga cgcatggctt ggctttaaag
     6541 gtacggactg gaaaaacgaa attaatgtcc gcgattttat tcaacataac tatacaccgt
     6601 atgaaggcga tgaatctttc ctcgccgaag cgacgcctgc caccacggaa ttgtgggaaa
     6661 aagtaatgga aggcatccgt atcgaaaatg caacccacgc gccggttgat ttcgatacca
     6721 atattgccac cacaattacc gctcatgatg cgggatatat taaccagccg ctggaaaaaa
     6781 ttgttggcct gcaaacggat gcgccgttga aacgtgcgct acacccgttc ggtggcatta
     6841 atatgattaa aagttcattc cacgcctatg gccgagaaat ggacagtgaa tttgaatatc
     6901 tgtttaccga tctgcgtaaa acccataacc agggcgtatt tgatgtttac tcaccggata
     6961 tgctgcgctg ccgtaaatct ggcgtgctga ccggtttacc agatggctat ggccgtgggc
     7021 gcattatcgg tgactatcgc cgcgtagcgc tgtatggcat cagttatctg gtacgtgaac
     7081 gcgaactgca atttgccgat ctccagtctc gtctggaaaa aggcgaggat ctggaagcca
     7141 ccatccgtct gcgtgaggag ctggcagagc atcgtcatgc gctgttgcag attcaggaaa
     7201 tggcggcgaa atatggcttt gatatctctc gcccggcgca gaatgcgcag gaagcggtgc
     7261 agtggctcta cttcgcttat ctggcggcag tgaaatcgca aaatggcggc gcgatgtcgc
     7321 tgggccgcac ggcatcgttc ctcgatatct acattgagcg cgactttaaa gctggcgtac
     7381 tcaatgagca gcaggcacag gaactgatcg atcacttcat catgaagatc cgtatggtac
     7441 gcttcctgcg tacaccggaa tttgattcgc tgttctccgg cgacccaatc tgggcgacgg
     7501 aagtgatcgg cgggatgggg ctggacggtc gtacgctggt gaccaaaaac tccttccgct
     7561 atttgcacac cctgcacact atggggccgg caccggaacc taacctgacc attctttggt
     7621 cggaagaatt accgattgcc ttcaaaaaat atgccgcgca ggtgtcgatc gtcacctctt
     7681 ccttgcagta tgaaaatgac gatctgatgc gtactgactt caacagcgac gattacgcga
     7741 ttgcctgctg cgtcagccca atggtgattg gtaagcaaat gcagttcttt ggtgcacgcg
     7801 ctaacctggc gaaaacgctg ctctacgcaa ttaacggcgg ggtggacgag aagctgaaga
     7861 ttcaggtcgg gccgaaaaca gcaccgctga tggacgacgt gctggattac gacaaagtga
     7921 tggacagcct cgatcacttc atggactggc tggcggtgca gtacatcagc gcgctgaata
     7981 tcattcacta catgcacgac aagtacagct acgaagcttc gctgatggcg ctgcacgatc
     8041 gtgatgtcta tcgcactatg gcatgcggca tcgcgggcct gtcggtggcg acggactccc
     8101 tgtctgccat caaatatgcc cgcgtgaaac caatccgtga cgaaaacggc ctggcggtgg
     8161 actttgaaat cgacggtgaa tatccgcagt acggcaacaa cgacgagcgc gtagacagca
     8221 ttgcctgcga cctggttgaa cgctttatga agaaaattaa agcgctgcca acctatcgca
     8281 acgccgtccc tacccagtcg attctgacta tcacttctaa cgtggtgtac ggccagaaaa
     8341 ccggtaatac gccggacggt cgtcgcgccg gaacaccgtt cgcgccgggc gctaacccga
     8401 tgcatggtcg tgaccgcaaa ggtgccgtgg cctcattgac gtcggtggcg aaactgccgt
     8461 tcacctacgc caaagatggg atctcgtaca ccttctcaat cgttcctgcg gcgctgggca
     8521 aagaagatcc agtacgtaaa accaaccttg tcggcctgct ggatgggtat ttccaccacg
     8581 aagcggatgt cgaaggcggt caacacctca acgtcaacgt aatgaatcgg gaaatgctgc
     8641 tggatgccat cgagcacccg gaaaaatatc ctaacctgac aatccgtgtc tctggctacg
     8701 ccgtgcgctt caacgcactg acccgtgaac agcaacagga tgttatttca cgtaccttta
     8761 cccaggcgct ctgacgccgg aggatgtatg aaaaagatta tcgaaacgca acgtgcccca
     8821 ggcgcaatcg gcccttatgt tcagggcgtt gatttaggca gcatggtctt cacctccggg
     8881 caaataccgg tttgcccaca gaccggtgag atcccggctg atgtgcaaga tcaggcgcgt
     8941 ttaagcctcg aaaacgtcaa agcgatcgtg gttgctgccg ggctgagcgt gggcgatatc
     9001 atcaagatga ccgtgtttat caccgatctg aatgattttg ccaccatcaa cgaagtctat
     9061 aagcagttct tcgatgagca tcaggcgacc tatccgaccc ggagctgtgt gcaggtcgcg
     9121 cgtttgccga aagatgtgaa gctggaaatt gaagccatcg cagtacgtag tgcgtaaagc
     9181 ctcgtgagcg ggacggtcgt aaggtcgttc cgctccactt cactgaacgg caatccgagg
     9241 gtgtggatat gattagtgca ttcgatattt tcaaaattgg gattggtccc tccagttcgc
     9301 ataccgtggg gccaatgaat gccggaaaaa gttttattga tcggctggaa agtagcggct
     9361 tattaaccgc gacgagccat attgtggtcg atctgtacgg gtcgttgtca ctgacgggca
     9421 aaggccatgc cacggatgtc gccatcatca tgggactggc aggaaacagt ccgcaggatg
     9481 ttgtcattga tgagatccct gcatttatag agttagtaac gcgcagcggg cggctgccag
     9541 tggcatctgg tgcgcatatt gttgattttc ctgtagcaaa gaacattatc ttccatcccg
     9601 aaatgttgcc tcgccatgag aacggaatgc ggatcactgc ctggaaggga caggaagagc
     9661 tattaagtaa aacctattac tctgtcggcg gcgggtttat tgtcgaagaa gaacacttcg
     9721 gcctgtcgca cgatgtcgaa acgtccgtac cttacgattt ccactcagca ggtgaactgc
     9781 tgaaaatgtg tgattacaac ggcctgtcta tatctggtct gatgatgcac aacgagctag
     9841 cgctgcgcag caaagcggaa attgacgccg gttttgcccg tatctggcaa gtgatgcatg
     9901 acggtattga acgtgggatg aacactgaag gcgtgctgcc tggtccgctc aatgtgccgc
     9961 gccgtgccgt agcgctgcgt cgtcagctgg tttccagcga taacatctct aacgatccga
    10021 tgaatgtcat cgactggatc aacatgtacg cgctggcggt tagtgaagaa aacgcagctg
    10081 gcgggcgcgt ggtaacggca ccgactaacg gtgcgtgcgg cattattccg gcagtactgg
    10141 cttattacga taagttccgt cgtccggtaa acgagcggtc aattgcccgc tattttctgg
    10201 ccgcgggggc tattggcgcg ctgtataaaa tgaacgcctc catctctggc gcggaagtcg
    10261 gctgtcaggg ggagattggc gtggcctgtt caatggcggc ggcagggtta actgaactac
    10321 tgggcggcag tccggcgcag gtatgcaatg cggcggaaat cgcgatggag cataaccttg
    10381 ggctgacctg cgatccggtt gccggacagg tacaaatccc gtgcattgaa cgtaatgcca
    10441 ttaatgccgt gaaagcagta aacgccgcgc ggatggcgat gcgccgcacc tcggcaccgc
    10501 gtgtttcact cgataaagtg atcgagacga tgtatgaaac cggcaaagat atgaacgata
    10561 aataccgcga aacatcacgc ggaggactgg ccattaaagt ggtctgcggc tgacattgct
    10621 gtcagctttc atccttgggt gcacaaatgt gcaccttttt tttgtgatct gccgcaaata
    10681 gagaatgaat attctcctgc gattaattta gtagaaaatt cttatccatc tccgttattt
    10741 caaagtcttg attgtgaaga ttttctctga cctcgcgcgt atcatttctt tattctaata
    10801 actcgcctgc aaaaacgtag accgcatatc gccttgatgc ggctcggaaa aaaataataa
    10861 gccctacaaa atattgagcg agaaattatg gaaattgcat cgaataaagg cgtcattgca
    10921 gacgcttcga ccccggcggg tcgtgctgga atgagtgaga gcgagtggcg agaagcgatc
    10981 aaattcgaca gtactgacac cggctgggtg attatgagta tcgggatggc gattggcgcg
    11041 gggattgttt ttctcccggt gcaggtcggt ttgatgggat tgtgggtatt tttgctctca
    11101 tcggtgattg gttacccggc aatgtatctg tttcagcgat tgtttattaa tacgctggca
    11161 gaatcaccag aatgtaaaga ttacccgagc gtcattagcg gttatttagg taaaaactgg
    11221 ggcatcctgt taggtgcgct ctatttcgta atgctggtga tttggatgtt cgtttattcc
    11281 accgccatca ccaacgatag tgcttcctac ctgcatacct tcggcgtgac ggaagggttg
    11341 ctgtcagaca gtccctttta tggtctggta ctgatttgca ttctggtggc gatctcctca
    11401 cgcggcgaga aattgttatt caaaatttcg accggcatgg tgctgaccaa gctgctggtg
    11461 gtcgcggcgc tgggcgtgtc gatggtggga atgtggcatc tgtataacgt cggttcgcta
    11521 ccgccgctgg ggctgctggt gaaaaacgcc attattacgc tgccgtttac cctgacgtcg
    11581 attctgttta tccagacgtt aagtccgatg gtgatctctt atcgctcgcg ggaaaaatca
    11641 attgaagtgg cgcggcataa agcattgcgg gcaatgaata tcgcgtttgg cattttgttt
    11701 gtcaccgtct ttttctacgc cgtgtcgttc acgctggcga tgggacatga cgaagcggta
    11761 aaagcctatg agcagaatat ttccgcgctg gcgattgccg cacagtttat tagcggtgac
    11821 ggcgcagcgt gggtgaaagt ggtcagcgtc attctcaata tctttgctgt catgactgca
    11881 ttctttggcg tctatttagg ctttcgcgaa gcaacgcaag ggatcgtaat gaacatcctg
    11941 cgtcgcaaga tgcctgccga gaagattaac gaaaatctcg ttcagcgcgg catcatgatt
    12001 ttcgccattt tgctggcctg gagcgccatc gtactgaacg caccggtgtt gagcttcacc
    12061 tctatctgta gcccgatttt cggcatggta gggtgcctga tcccggcgtg gctggtttac
    12121 aaagtaccag cattgcacaa atacaaaggg atgtctctgt acctgattat cgtcactggt
    12181 ttgttgctgt gtgtttctcc gttcctggca ttttcttgat ttacctgaaa ttttaaggtt
    12241 tttaatatgt ttgattcgac tttaaatccg ttatggcagc gttacatcct cgccgttcag
    12301 gaggaagtaa aaccggcgct gggatgtact gaaccgattt cactggcgct ggcggcggcg
    12361 gttgctgcgg cagaactgga aggtccggtt gaacgtgtag aagcctgggt ttcgccaaat
    12421 ctgatgaaga acggtctggg cgtcaccgtt cccggcacgg gaatggtggg gctgccgatt
    12481 gcggcggcgc tgggggcgtt aggtggaaat gccaacgccg ggctggaagt gctgaaagac
    12541 gcaacagcgc aggcaattgc cgatgccaaa gcactgctgg cggcggggaa agtctccgtt
    12601 aagatccagg aaccttgcga tgaaatcctc ttctcacgcg ccaaagtctg gaacggtgag
    12661 aagtgggcgt gtgtcaccat cgtcggcggg cataccaaca ttgtgcatat cgagacgcac
    12721 gatggtgtgg tgtttaccca gcaggcgtgt gtggcagagg gcgagcaaga gtctccgctg
    12781 acggtgcttt ccagaacgac gctggctgag atcctgaagt tcgtcaatga agtcccgttt
    12841 gcggcgatcc gctttattct cgattccgcg aagctaaatt gtgcgttatc gcaggaaggt
    12901 ttgagcggta agtgggggct gcatattggc gcgacgctgg aaaaacagtg cgagcgcggt
    12961 ttgctggcga aagatctctc ttcatccatt gtgattcgta ccagcgcggc atccgatgcg
    13021 cgtatgggcg gcgctacgct tccggctatg agtaactccg gctcgggtaa ccaggggatt
    13081 accgcaacaa tgcctgtggt ggtggtagca gaacacttcg gagcggatga tgaacggctg
    13141 gcgcgtgcgc tgatgctttc gcatttgagc gcaatttaca tccataacca gttaccgcgt
    13201 ttgtctgcgc tgtgtgccgc aacgaccgca gcaatggggg ccgccgccgg gatggcatgg
    13261 ctggtggatg ggcgttatga aaccatctcg atggcgatca gcagtatgat cggcgatgtc
    13321 agcggcatga tttgcgatgg tgcgtcgaac agctgcgcga tgaaggtttc gaccagtgct
    13381 tcggctgcgt ggaaagcggt gttaatggcg ctggatgata ccgccgtgac cggcaatgaa
    13441 gggattgtgg cgcatgatgt tgagcagtcg attgccaacc tgtgtgcgtt agcaagccat
    13501 tcgatgcagc aaacggatcg gcagattatc gagattatgg cgagcaaggc cagataaacg
    13561 ccaggttttg gggatcgtgc cggatgcgat gctggcgcat cttgtccggc ctacgggttg
    13621 tttaattcgt taatgttgtt ggattttgta ggccggataa ggcgttttcg ccgcatccgg
    13681 caacctgtct ttacgcggta gcttcatcct cggctaaacg cgtttcagca tccgcgacga
    13741 tagcttccag ctcgctcaac atctcttcat agccaaaatt tttggcggtg cgagcaggtt
    13801 ctttcgccac cacgggcttc accggctggc gctttttggc caatttttta ctcatcatca
    13861 tctccctgta aaaacaacta gacaggcaaa tctatcagca aagcgcgcag tggggaatcg
    13921 gcaaccagcg taatgttagc ctcgtcacga ataaacgccc catcaccgca ggtcagcgcc
    13981 gctttctctt catgatgcgt aagcgcatga aatttcccgt gaatcgattg caaatacgcg
    14041 cgtggcccat gcaactggaa attcgcactt tcgcctttgt cgagcacgat atggtgcagc
    14101 cacacttgct ggcgtaattg caggcttccc atcgcaccct ctggcgaggc gattaattgc
    14161 tgcttgccca tattaagcgc cagcttttga atcagcggat tctctcgctg cgggcaggcg
    14221 tccagccaaa gctgcattcg cgttaacggt ttgtctttgc tgagattgtg ttcgctatag
    14281 ctgacacccg gctgggtaga gagcagcaac gcctctccgg cgctggcctg aacatgattg
    14341 ccttcgctat cgcgatactc tgcttctcca tccagaatca catttaaaat atcgactttg
    14401 ggataggttc gcggctgaaa ggcggcacct ggggccagca cttcctggtt aagcacacgc
    14461 agggaggcat agcctaacaa tttcgggtcg aagtagtgtc caaaggaaaa agtataccgg
    14521 gcctgcaacc atccgtagtc tgcttgtcca cactgcctgg cagttcgggt agtaatcata
    14581 attcttgacc tctctttact tccttttatg gtaaggggct ggacgctaca ttgttagcca
    14641 gatattctgc ccggtatgtt caaatttcct gaatgagaac gaaatggcca aagaaagggc
    14701 attaacgctg gaagcactac gggttatgga tgcgatcgat cgccggggca gttttgcggc
    14761 ggcggcggat gagctgggac gcgtgccttc cgcacttagc tacaccatgc aaaaactgga
    14821 agaagagctg gatgtggtgc tgtttgaccg ctcgggccat cgtaccaaat tcaccaatgt
    14881 cgggaggatg ttgttggagc gagggcgcgt tttgctggaa gccgcagata aactgactac
    14941 tgatgcggaa gctctcgcgc gcggttggga aacgcatctc accattgtga ccgaagcgct
    15001 ggtaccgaca cctgcctttt tcccgttaat cgacaaactg gcggcaaaag ccaataccca
    15061 actggcaatc atcacagaag tgctggcggg ggcgtgggaa cggctggagc aggggcgggc
    15121 ggatattgtt atcgcgccgg atatgcattt tcgttcctcg tcggagatca actcgcgcaa
    15181 gctctatacg ttaatgaacg tctacgttgc cgcgcctgat cacccgattc atcaggagcc
    15241 ggaaccgtta tctgaagtga cgcgtgtgaa atatcgtgga attgcggtgg cggataccgc
    15301 tcgtgagcgc ccggtgttga ccgtacagct gctggacaaa cagccgcgct taacggtgag
    15361 cacgattgaa gataaacgtc aggcattact ggcggggctt ggcgtggcga cgatgccgta
    15421 tcctatggtc gaaaaagata ttgcggaagg gcggttgcgt gtcgtcagcc cggaatcgac
    15481 cagcgagatc gatattatta tggcctggcg tcgtgacagt atgggggaag cgaaatcctg
    15541 gtgtctgcgg gaaattccca aactttttaa cggaaaataa tatttattat tgttaataaa
    15601 aaggggcgaa atatcgcccc aattctaaga ttaatttgaa ccaaacttcg gatcgtttcc
    15661 gtaacgattg ctgccagaag taccttctgt gcagaaaaaa accagaagca cgagccagcc
    15721 aataaacgga acaaaaaaga gcaaggccca ggcacctgaa cggtcggtat cgtgtaagcg
    15781 acggatagca agcgcaagga cgggaaggaa ggttgccagc aaataaagca tagacagata
    15841 tggaagctcc agacctaaaa tcaattgaat gacattgata atagcgccga cgatggcgtt
    15901 aatcagagta aacatccagt actctttacg acgtgcacga ccagagaaac caacataatt
    15961 ttttaatacg gctaaatacc actgcataaa aacatccctt taattaaaag tgacgaaagt
    16021 ctattccggg tattgtgtaa atgctattcg tatctttctt tttcggatgt tataaaggtt
    16081 ttgatattta agataaatac atggttgttt tttataggta atttatataa cgacattgaa
    16141 atattttaaa taaacattag taaattattc attcatcaac atgatgataa aagccggatg
    16201 agtcagacat ccggcgagca gatttgtttt aaggttccag ttttggatca ggtccaaagc
    16261 ggttttcgcc tggcgtaccc gcctggcagt taaagacgat gataataagc cagccgataa
    16321 acggaattaa gaatagcagt gcccaccacg ccgagcggtc ggtgtcgtgc aggcggcgga
    16381 actgaactgc ccaccacggt aaaaacacca gaataccgta gattgtcgtc aggatgcctt
    16441 cgccaccagc gcgttgccag cctaacattt tatccagcag ccccagcacg aacgtaaaga
    16501 tgatgttgac cagaataaac atccagtact ctttgcgccg cgcacgaccc cggaaaccga
    16561 cataattttt tagtactttc agataccagt ccatttttgc ctcaattttg ctcaatcact
    16621 tgtttttaaa gcagcaaatt aagaatagac gtgaatgaaa tggcagtaaa tattatctcg
    16681 atgaattaat aaggtgctgt gaaggataag ggcaagcgca aaattgccag atgcgctacg
    16741 cttatctggc ctacgcggcc tttgcaacat attgaatata aacgttttgt aggtttgata
    16801 aggcgttcat gccgcatccg acatgaacaa catgcactta gctaattatc agaggcacct
    16861 cgtaaggtgc ctttttttaa ccgaagcgaa catctcgtcc atgcggttca tcgagatcct
    16921 gccacggacc aattgaaata atccccgtag ggttgatggt cttatggctg cggaagtaat
    16981 gattacggat atgatcgaaa ttgactgttt cggcaattcc cggcatctgg tagatatcac
    17041 gcaggaagcc atacagatta aggtaatcgc tgatgcggta cttatcacac ttgaagtggg
    17101 tcacatacac cggatcaaaa cggaccagcg tagtccacag gcgaatatcg gcttcggtta
    17161 gctgattgcc ggtcaggtaa cgatgctggc ctaaaatctg ctccagtcgc gccagcgatt
    17221 caaacacttt cgccaccgcc tcgtcgtaag cttcctggct ggtggcaaaa ccagctttat
    17281 acacgccgtt gttaacggtg tcataaatcc agccgttaag ttcgtcaatt ttcgtttgca
    17341 gggctggtgg gtagtaatct ccagctttcg cacccagcgc atcaaacgcg gtattgaaca
    17401 tgcgaatgat ttctgctgat tcgttgctga cgatggtatg gttctttttg tcccacagca
    17461 cgggaacagt aactcgtccg ctgtagtgtg gatcggcgtg gagataaagc tgatacagaa
    17521 attcatgttg atagagcgta tctccggttg ctcccggaaa actgtcatca aaggtccagc
    17581 cgttttccag catcagcggg ttcactacgg aaacggaaat aaacggttcc agtcctttga
    17641 gtttgcgcat gatcagcgtg cggtgcgccc acgggcaggc gagtgaaaca tagagatgat
    17701 aacgatcttt ctctgcgata aaaccgcctg tgccagtggg gccaggagcg ccatcggcag
    17761 tgagccagtt acgaaatgcg gaagctgaac gttgaaattt accgccggta gatttggtat
    17821 cgtaccaggt gtcatgccag acgccgtcaa tcagttgacc catttttctc tcctccaata
    17881 gcaaaaacga ggagcttcct cctcgttttt tatcgttaag tatagcgcgt gcttaccact
    17941 ttttattcag caggcggtcg atgctatacg cgcccggacc ggtaattgcc agcagcagga
    18001 atccgccaga aattgtcagg tttttcatga acatcagcga gttgacgcct tcagcaaagt
    18061 tgctgtgaaa taaaaatgcc gtcagcagcg taaagcccgc agtaaacagg gctgtggtgc
    18121 gagtcaggaa accgaacagg attgccagac caccaccaaa ctcaagcaga atcaccagcg
    18181 gcagcataaa acccgagacg cccattgctt ccatatattg ttgggtaccc gcatagccag
    18241 taatttttcc ccagccagcg gtaataaaca gaatcggcat taaaatgcgc gctaccagta
    18301 caccaacatc ttctaatttt ttcatcatac tctccactgc gccactcagg ctgctgattg
    18361 tttttttgtg caatggcgcg gtattagcgt cgttgctgtc gatggagaga atcataaacg
    18421 tggtgaatga tgattgttag caaggaaaac tgtcaaaaat cttcaaaaaa tttgagggat
    18481 aaggccggaa tggctccggc cagagggaag ttaaccgcga agctgttgct gcttgagggt
    18541 cgttttaacc agacgccagg cgctccatac gccaaaaccg cgtctggccc agcggaccag
    18601 catattagga tggcgaatcg tccagatcgc catcacgcta ctgccaacca gcgcccagga
    18661 gcgcagactt agcagcatat tccagcgacg atcgtaagcg cctgttgtct ccagccattc
    18721 acgacgactg gcggaaagat ccagccgttg ttgctggatc tggctaagca gttgcgcctt
    18781 acgtcgttca cgttcgactt tactgctcac gggactcctc ctcgagcagc tgccgatcgt
    18841 ttgccagctc atggcgtgta tggcgcagca aggtggattt acgcgattta cgtaacgtcc
    18901 agataccgcc aatcagtgcc agtagcagca acaccacggt ggtggcaatc atcgcattca
    18961 ggcgatattg cggatcaacc gcccaaataa ttagcaccat caggctcata agaccaaatg
    19021 cagcgaaaag catcgtcagg cccagcatca gtaaaagttg aaagagatta gctttttcct
    19081 cttccagctc aaccaccgcc agccgcagac gtgtttccac catttcaacc atgatggaaa
    19141 caattcgctg cccgatgccc agaacgcttt taccgggccc ttgcgcgtga tgagtgtccg
    19201 ccataattaa cgacgcgaca gcagaacgcc gagcactaca ccgattgcag cgccaatgcc
    19261 cacgcccgtc catggatttt cacgcacata ctcatcggca cgcgccgccg cgacacgggt
    19321 ttgtttggcg atggcatcac cggtttcacc caggcgataa cggctctgtt tcagtgcctg
    19381 ctccgctttg ctacgaatct tactcaactc ttctttcgac ttctcaccag atgagctaag
    19441 cacctcttcc agcgtatcgg aaagggattt caactcagca cgcagatgtt ccgtagtgtg
    19501 ttctttcgac atagttttct ccaggtgagt aagtagtgac tattgtgagt tagtagtcgc
    19561 gcgcttccag ctttttcagc tcttcctgcg cttctgccag tttccgttcg cgtttggcaa
    19621 tcttatcggc atcgcctttt tgcttcgcct cggctaaatc ttgctggcgt tccgccacct
    19681 catctttctg ctttgcgatt ttcttctgat gatcggcacg cagctggcta tctgaacagt
    19741 tggcccggac ttcactcagg gctttattca gaccgtcaat acgattctgg ttttggtgtt
    19801 tttcggcata gctgatctcc ttaaggatat tttgctcctt ttcctgacac agggtagtgg
    19861 cataactacc agcactaaga gcaaaaagag aaacagctaa agcgatgcgg tatttcattc
    19921 ttgaaccttc cattgtgatt cggacctatt acaaaggaac gattgtccag acaacgaagc
    19981 ggtagtgtta accaccgcct cattaatcat agacagttac ggtgaaatca ccaaagtgag
    20041 tgatctgttt cggataacag gaagttatcc gaagcgatga gagttatccc gtagccgggt
    20101 cagccactgc atagcctgac tgttattgtc ctgctgcgca atgatgtagc cttggggcaa
    20161 tgttctgtcg aggaccgctt ttgcagcggc gctctggtcg ctggagtcaa aggtaattaa
    20221 cagagtgtcg tttttggggg tgatactttt gaaaggaatg ccatgagcgt caaggtgatg
    20281 ccagattgaa aaaccgtctg gcatcgttgt gccttgatga acggcacgaa tcgccagcgt
    20341 agactcttgc tggcgaaccg cggaccaggc cagcaacagc gtgccgacca gaagtaaaac
    20401 agcgccggac caggctagct ggcgaagcga catgcgaggc atttgcatcc cttacccccg
    20461 atttccatat ttctttttcc ataacacgac cagagaacct gccaggccaa acaccagcag
    20521 caccaccggg agcagcatca ggcatgacat cagctggtcc tcgtacttta aaaataccgg
    20581 cgttttgccg agcatgtaac ccagagttgt caggatcaat acccacagca gaccgctcat
    20641 ccagttgaaa aactgaaagc gcgcgttatt cagccctgat aacccggcaa tcgtcggcag
    20701 cagtgttctg acaaacgcaa taaagcgacc aattaacagc gccgataaac cgtgtttatg
    20761 aaaaagatgg tgtgcgcgtt gatgataatg cgcgggtaaa tgagatagcc agttttgtac
    20821 ggtgcgggta ttgcccagcc atcgcccctg aatatagctg acccagcagc cgaggctggc
    20881 ggcaacggtc agcagcagaa tcgtttgcgg atagcccatc gcgcctttcg caatcaacac
    20941 gccgaccaat accagtaaac tgtcgcccgg taaaaaggcc gccggaagca agccgttttc
    21001 aaggaacaaa attacaaaca agacaaaata caacatgcca atcatcgatg gattggccag
    21061 ggtttcaaaa tcctgcgccc acagggcttg cagcaattgg gtcaaaagtt ccattcatta
    21121 ttcctggtga atcggttaac gccacgctgg ttttaaaaga tgcactaccg caatgttatt
    21181 gctgttttgg tatgttctgc gggtttgata catcaacaca gcctgaacta ttccctgtta
    21241 aatcgtgaga taagaaagca ttcaaaaaca taaattcaaa ttgaatctaa ttgtaacaaa
    21301 ggtcaacgtt gttcgttatc aaaattacat gtccgtggag tctgattttg ctgaatgcag
    21361 gggagggtgg cgagaggatt tacacaggct gacactttat acttttcgct taccgacgag
    21421 acaaacaggc gcattgcagc gcctgtcgga caggaatttc atttactgcc gctggtggct
    21481 gtatcgagat gcaccaccgg gttttcggcg aatagatagc ggtcggcatt gaactcgaag
    21541 tcgtcgctgg tttcgttaaa taacatgatc ttggtgtttt ccagatgctg ccacattgcc
    21601 agcttagcag catgaggatc tttgcgaatc agcgccttga ggatttgatc gtggtcatca
    21661 caccagttat cgacggtacg ggaatcaatg tgttcgtgca gttttttcca gtacgggtta
    21721 tgactacgct gggtccacat tttttccacg atagccgcca gggcggagtt ctgcgtcgcc
    21781 agagcgacct gaatgtggaa ctgcaaatcc cactcggaat cacggaagca ttgttcgccg
    21841 cgcgcctgtt cctgaatggc catcagtttc atgatgtcct gtttcgttac ctgagttgcc
    21901 gcgaattcgg caatattact ttcgatgagc tggcgagcct gaagcaactc aaacggaccg
    21961 taattggcga attccatatt attgtcagcc gcctgctgat ggcgcggctg gttggaaacc
    22021 acatgaatac ctgaaccttt acgcacttcg acatagcctt caacttccag catgatgatg
    22081 gcttcacgaa ctaccgtacg gctgacgttc ttttcatcgg caataaagcg ttctgcaggc
    22141 agtttatcac ccaccagata gacgccctgt tcgatgcgct ctttcaggtc agcggcaagt
    22201 tgttgataca aacggcgtgg ttcagtgatt tccatatgcg ctccaggcat gatacggcag
    22261 actatatttg ttataccact tttgcgagtt tagctctact cgcagtgatg aaaaagccgc
    22321 ctgagaaggc ggctttgaag ggaaactaaa acttacacga aacgttaatg ttgcggtgcg
    22381 ggatcgttat gtgtttcctg cgccacctca atcgccggtt tgttctgcaa cacggtccag
    22441 ataaccagcg cacctaacag gtcgaacact gccagaactg cgaacagcgg gctgaagccg
    22501 atggtgtcag ccagtgcacc gacaaccagc gcaaacagcg tacttgccag ccatgcggac
    22561 atcccggtta aaccgtttgc cgttgccact tcgttacgac caaacacatc ggaagagagc
    22621 gtaatcagcg cgccagacag tgcctggtgg gcaaaaccac cgatacacag cagcataatt
    22681 gcgacatacg ggttggtgaa caggccgatc atacccgggc caatcattag cactgcaccc
    22741 agcgttacga ccatcttacg ggaaacgatc aggttcacac caaaccaacg ctggaacagc
    22801 ggcggcaggt aaccaccgag gatacaaccg aggtcagcaa acagcatcgg catccaggcg
    22861 aacatcgcga tctctttcag gttaaagccg taaactttaa acatgaacag cgggatccac
    22921 gcgttaaaag taccccaggc cggttctgcc aggaaacgcg gcagcgcgat accccagaac
    22981 tgacggttac gcaggatctg accaacggac attttcttcg ccgtgctcac ctggtgctgg
    23041 gcttcctgac cattaataat atagtcgcgt tcttcatcgg tcagatgctt ctggtcgcgc
    23101 ggatgtttat agaaaatcag ccatgccatc gcccagataa agctcaatgc accggagatg
    23161 ataaatgcca tctgccagct gtgcattacg attgcccata ccaccagcgg cggcgcaatc
    23221 atcgcaccaa tcgaagaacc tacgttaaag tagcctactg cgatggaacg ctctttcgcc
    23281 gggaaccatt cggagctggc tttcagaccc gccgggatca tcgctgcttc cgcggcaccg
    23341 accgcaccac gagcaacagc caggccaccc cagctacctg ccagcgcggt tgcaccacag
    23401 aacacggccc acagcacagc aaacattgca taaccgattt tcgtacccag cacatccagt
    23461 acataacctg ctaccggttg catgaccgta taagcagcag aataggctgc gatgatatag
    23521 gaatactgtt gggtggagat gtttaactct tccatcagag ttggcgcagc tgccgccaca
    23581 gtgttacgcg tcaggtaacc aagcacggtg ccgagcgtca ccagtgcgat catataccaa
    23641 cgtaaccctt taattttacg catgtaaaac ctcatcgttt tgttatatcc gcaggaagcg
    23701 cggccatcat cccgccagag gctgaagatg ttagttacga ccgggtaacc gaaaaaaaac
    23761 cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat tcggcttccg taccggttaa
    23821 cccgattcat ctaacaaatg aatgaatcgg caatttgcat tccgtcgact tatgatttgc
    23881 gacggcagaa agataacttg tcatacaact ttaaaaggtg agagccatca caaatgtggg
    23941 aatatttgta gggacattac ctgacgacag caaggccagt attggcgcgg cctgcagcga
    24001 gatttaccac tttgagagta atttttttaa ctacgtttat tgatctaact cacgaaaata
    24061 tcttcggact ctggaaattg gtgtgataac tttgtcagca tcgcaccata agcaagctag
    24121 ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg actgaagatt tcctgttaga
    24181 taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa gaccagccga ttttcgatta
    24241 ccattgccat ttgccgccgc agcagattgc ggaagactat cgttttaaaa acctatatga
    24301 catctggctg aaaggcgatc actacaaatg gcgcgctatg cgtaccaacg gtgtggccga
    24361 gcgtctgtgt accggtgatg cgtctgaccg tgaaaaattt gacgcctggg cggcgactgt
    24421 tccgcacact atcggcaacc cgttatacca ctggacgcac ctcgaactgc gtcgtccgtt
    24481 tggtatcact ggcaaattgc tttctccgtc aactgccgat gaaatctgga acgaatgtaa
    24541 cgaactgctg gcgcaggata acttctccgc gcgcggcatc atgcagcaga tgaacgtgaa
    24601 aatggtcggc accaccgatg acccgatcga ttctctggag catcacgcag agatcgccaa
    24661 agacggctct ttcaccatca aagtgctgcc gagctggcgt ccggacaaag ccttcaacat
    24721 cgaacaggcg acctttaacg actacatggc gaagctgggc gaagtttccg ataccgacat
    24781 tcgccgcttt gctgacctgc aaactgcctt gaccaaacgt ctggatcact tcgccgctca
    24841 cggctgtaaa gtgtctgacc atgcgctgga cgtggtgatg tttgctgaag cgaacgaagc
    24901 ggaactggac agcattctgg cgcgccgtct ggctggcgaa accctgagcg agcacgaagt
    24961 ggcacagttc aaaactgcgg tactggtgtt ccttggtgcc gaatatgcac gtcgcggctg
    25021 ggtacagcag taccacattg gcgcgctgcg taataacaac ctgcgtcagt tcaaactgct
    25081 ggggccggat gtaggctttg actccatcaa cgaccgtccg atggcagaag agttgtctaa
    25141 gctgctgagc aagcagaacg aagaaaacct gctgccgaaa accattctgt actgcctgaa
    25201 cccgcgcgat aacgaagtgc tgggcaccat gatcggtaac ttccagggcg aaggtatgcc
    25261 gggcaaaatg cagttcggtt ccggctggtg gtttaacgat cagaaagacg gtatggaacg
    25321 tcagatgacc caactggcgc agctcggttt gctgagccgc tttgtcggta tgctgactga
    25381 cagccgtagc ttcctgtcat acacccgtca cgaatacttc cgccgcattc tgtgccagat
    25441 gatcggtcgc tgggtggaag cgggcgaagc accggcggac atcaacctgc tgggcgagat
    25501 ggtgaaaaat atttgcttta acaatgcgcg tgactacttc gccattgaac tgaactaagg
    25561 tctggggttg atatgcaata catcaagatc catgcgctgg ataacgtcgc ggtcgcttta
    25621 gcggatttgg ctgaaggcac agaagtcagt gtcgataacc aaactgttag gctgcgccag
    25681 gatgttgctc gtggacataa atttgcgtta acgaatatcg caaaaggggc caacgtcatc
    25741 aaatatggcc tgccgattgg ttatgcattg gcggatattg cggcggggga acacgttcac
    25801 gcccacaata cgcgcacgaa tctgagcgat ctggatcagt atcgctatca acctgatttt
    25861 caggatctgc ctgcgcaagc ggcagatcgt gaagtgcaga tctatcgtcg cgctaacggc
    25921 gatgtcggag tgcgtaatga gctgtggatc ctgccaaccg tgggctgtgt caacggcatc
    25981 gcgcggcaga tccaaaatcg tttcctgaaa gagaccaaca acgccgaagg tactgacggc
    26041 gtgttcctct tcagccacac ctacggctgc tcacagctgg gcgacgatca catcaatacc
    26101 cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcgg gcgcagtgct ggtgattggt
    26161 ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa cgcttggcga tatcgatcct
    26221 gaacgcgttc atttcatgat ctgccaacag caggatgatg agatcgaagc cggaatcgag
    26281 catttgcatc agctgtataa cgtgatgcgc aatgataaac gcgagccagg caaactcagc
    26341 gaactgaagt ttggtctgga gtgcggtggt tctgacggtc tttctggtat tactgctaac
    26401 ccgatgctgg ggcgtttctc tgactacgtg attgctaacg gcggtactac cgtactgacc
    26461 gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg accattgccg cgacgaagca
    26521 acgtttgaaa aactggtcac catggtcaac gacttcaaac agtactttat tgcccatgat
    26581 cagccgatct atgaaaaccc atcgccgggg aacaaagcgg gcggtatcac cacgctggaa
    26641 gacaaatcac ttggctgtac ccagaaagcg ggttccagcg tcgtggttga cgtgctgcgt
    26701 tacggcgagc gtctgaaaac gccagggctg aacttgttaa gtgcgccggg taacgatgcc
    26761 gtagcgacca gcgccctggc gggtgcgggc tgccatatgg tgctgttcag tactggtcgt
    26821 ggcacgccat acggcggctt tgtaccgacg gtgaaaatcg ctaccaacag tgagctggcg
    26881 gcgaagaaaa aacactggat cgactttgac gcgggtcagc tgatccacgg caaagcgatg
    26941 ccgcagttgc tggaagaatt tatcgacacc atcgttgagt ttgccaacgg taaacaaacc
    27001 tgcaacgagc gtaacgactt ccgcgaactg gcgatcttca aaagcggcgt aacgctataa
    27061 agtcgaaatg agttgttaat aaaaaacggc gtttcataat gtgaagcgcc gtttgctttt
    27121 ccatacctga taaggattta tcatgaccgc gtattggctg gcccagggcg tgggtgtcat
    27181 cgcctttctg attggtatca caacattttt caatcgtgac gaacgtcgct tcaaaaagca
    27241 gctttcggtc tatagcgccg ttattggcgt acattttttt cttctgggca cctatcccgc
    27301 tggtgccagt gccatcctta atgccattcg tacattgatt accttacgca cgcgcagctt
    27361 atgggtaatg gcgattttta ttgtgctgac tggcggaatt ggtctcgcga agttccatca
    27421 tcctgtcgaa ctattgccgg ttatcggcac gattgtcagt acctgggcgc tgttccgctg
    27481 taaagggctg accatgcgct gcgtaatgtg gttttcaacg tgttgctggg tgattcacaa
    27541 cttctgggcg gggtcgatag gcggcacgat gattgagggt agttttctgc ttatgaatgg
    27601 cctgaatatc atccgtttct ggcggatgca gaaaagggga attgatccgt ttaaagtaga
    27661 gaaaacccct cccgccatag acgaaagggg ttaagcaatt aattacgcag ggcgctattt
    27721 gccagacgac cgtcttctgc ctggcaagct gccgcagtga acagcacgtc agttgaagag
    27781 ttcagcgcgg tttcgcaaga gtcctgcaat acgccgatga taaagccgac ggcaaccacc
    27841 tgcatggcga tatcgttcga aataccgaac atattacagg ccagtgggat cagcagcaga
    27901 gacccccctg ccacgccgga tgcgccacag gcacacagag aagccactac gctcaacagc
    27961 agcgctgtgg gcagatcgac cggaataccc agcgtattaa ccgcagccag cgtcaacacg
    28021 gtaatggtga ttgctgcgcc cgccatattg atggtggcac ccagcggaat agaaacggaa
    28081 taggtatcgc gatccagatt cagcttttca cacagcgcca tattcaccgg aatgttagct
    28141 gcagagctgc gggtgaagaa ggcatacacg ccgctttcgc gcaggcacag cagcaccagc
    28201 gggaacgggt tacgacgaat tttccaccac accagcaatg ggttaaccac cagcgccacc
    28261 agtaacatac agccaaccag cacgaccagc agttgcgcgt agccccacag tgtggagaaa
    28321 ccggtggttg ccagggtaga agaaaccagc ccaaaaatac cgatcggtgc gaagcgaatg
    28381 accagtttca ccataaaggt gacggcattc gacatatcgt tgaccaggtt tttggtggtc
    28441 tcgttaccgt gacgcagtgc gaagccgagg ccaattgccc acaccagaat cccgatgtag
    28501 ttacctttca gcagcgcgtc gatggggttg gaaaccatgc tcattaccag cccgcgcatc
    28561 acttcgacaa tgcctgacgg cggcgaaata tcacccgcgc tactggataa atgcagggta
    28621 gaagggaagg caaagctgaa gactactgcg gccagagcag cagagaaggt gcccagtaga
    28681 tagaggaaca aaatagggcg gatattggtt ttctgcccgt gctggtggtt agcaatagat
    28741 gccatcacca gcatcaacac caggatgggg gcaacggctt tcagtgcgcc gacgaacaaa
    28801 gtacctaaca gaccaacagc ttccgccgcg ggttttgaga tccatgccag aagaatcccc
    28861 agaacaaggc cgaccaggat ttgttttacc aggctgccat gagccagacg ccggaatagc
    28921 cccggtgaac gttgcgtagt catttttcga tcctttcatt gtgttgtcgc acatccctgg
    28981 tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt ttgcaaggtc gagtataagg
    29041 aaagttacag cttcgggaag cgaaaaatgc tggattttaa agagaggtca tgttttgcaa
    29101 cttaattgtt acatttattt gacataaaaa taaagccaac gtgcggcttc cagactgatg
    29161 acaacatcaa aaaaggactc ggtcagtccc ctcgccccgt ttggggagag ggttagggtg
    29221 aggggaacag gccagcatcg gtgcgaaaaa ttaccctcac cccgaccctc tccctggaag
    29281 ggagagggag aaaacggtgc tcatgttaat gacagggtac acggcttagg cagattaaaa
    29341 attatccacc ccgctgctta tcatgccgat aattcaccca ggcgttgata ataaacgtca
    29401 tcaccagaat gccaaacacc acgcccagcg agacggcgat tggaatatgg tagaagtcga
    29461 caatcagcat cttgataccg ataaacacca gaatcaccgc caggccatat ttgagcatcg
    29521 agaaacgctc tgctacgccc gccagcagga aatacatcgc acgcaggccg aggatcgcaa
    29581 acaggtttga ggtcagcaca atgaacgggt cagtggtcac ggcgaagata gccggaatgc
    29641 tatccacggc gaaaatcacg tcgctcaatt ccaccagaat cagtaccagc atcagcggtg
    29701 tggcgtacag caatccattc ttacgcacaa agaaatgctc gttgtcgatg gtgtcggtca
    29761 tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt
    29821 catgggcgag cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga
    29881 tccagtcgaa ctgcgaaatt agccagctgc cagtgaagat catgatggta cgcagaacaa
    29941 tcgccccgag cacgccatac accagcacgc ggcgttgtaa tgccgccgga acagagaaat
    30001 agctgaacaa catcagccag acaaagacgt tatcgaccgc cagcgatttc tcaatcagat
    30061 aacctgtgag aaaggccagt gcctgtggat cggcaacggc gcgaccttcg gtttgcacca
    30121 gataccacca gaaagcggcg ttaaacagta acgacagcgt cacccagacc agcgaccagg
    30181 ccgcagcctg tttcatggtc atggcatgtg ccccacgacg cccctgcaac aacaggtcga
    30241 tagccagcat aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag
    30301 tattcataga agttccttac acataaaaaa acggccaacg tcggaagacg tcagccgctg
    30361 ctttttatgc atagacctcg ccttccggca aggtctcact tacaacaaaa aaggaatacg
    30421 tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg tttttcacac atcgtaatga
    30481 cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac aaagtatgac agaccattca
    30541 gccagtcaat ggtctgttac atacctttta cactgtttta ctttggcaat tatagtttta
    30601 cgctatctgc cggaaaaatc accccagtct ggcggcggat ctcggtcagc agtttggcgg
    30661 tgatgcgact gaccgccagc cctggatgat ccaccagatg ctcatccacc agggtagcga
    30721 acagctctgc ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga tcctgcattt
    30781 ggctgccacg cggcacgaag cacactttct ggcattcaga cagtttttca atcaccagcg
    30841 atcctgcttc gccctgaatc tcgctcgcca ggacagaatc actgactttt gagtgctgca
    30901 aggtgacgct gaaatcaccg taatccatca ccaccacgcc ctgggcgtca acgccgcttg
    30961 ccagcaaact ggcggttgcc tgcacgcttt tcggctcacc aaataacgcc accgccgacg
    31021 ccagacagta aaaaccgata tccataattg aaccgttaga gaatgccgga ttaaaggtgt
    31081 tgggattctc accatccagg taacgttgat accgcgagga atactggcaa tagttgaaaa
    31141 agactttccg cagtttgccg actttcggca gcgcctggcg caacaaatga aagttcggca
    31201 ggcaggcggt tttaaatgcc tcaaacagca ccacctgatt ttcccgcgca caggcaatgg
    31261 cggcatccac ttccgccaga ttcgacgcca gcggtttctc gcaaatcaca ttaattttat
    31321 ggctaaggaa aagttgtgtc tgggaaaaat gcagggaatt cgggctggca atatacaccg
    31381 cgtcaatggc atcgctttcc gccatcgctt ccagcgaggt aaacagatgc tcgacagaaa
    31441 aatcattggc gaagtgctgg gcctgttcaa ggctgcggga atatacggcg gttaacttgt
    31501 atttaccgct ctcatgggcg gcctcgacga actggcgagt gatccagttc gtaccaatca
    31561 cagcgaaacg tatcataacg cgtggatact ccataaaggg attctgtcgt caatgtagca
    31621 cgcctttttc atcttcgacg tgcgcagata cgcattattt ctgcgccgga cagccatgct
    31681 cactaaacgg cccaatcatg accgaaggcc cgagatattt cggctggcga tggaggattt
    31741 tggcgtcaat cacggcgctg acacgagcga gtttctcccg cagttgagta taaaaaccgt
    31801 gcttcatatt gaggtctggc gcgttaaaac cgatagcgtc gatgccgtac tgttttgcca
    31861 gccagatggc gcgttgatta tgaaattctt gcgagataat ggtgatatgg ttttcaccaa
    31921 aaaccttttt ggcacgcact accgaatcca gcgttgagaa tccggcatag tcgcagaaga
    31981 tcacttttgc tggtacgcct ttagcgatca aagcctgctg catacctgat gcttcatcgt
    32041 aatttttacg tccgttatcg ccacttacca gcagccattt cacttttccg gcgtggtaca
    32101 acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctc gcccccagca
    32161 ataaacccac gttacgcgcc ggaacggcgt tgacattgct ccaggtcagc tgtttgctgg
    32221 cgttgaccat tacgcgatcg gcgataagga tggtcgctcc tgccacaagt aacagtaagc
    32281 aggcaatctt tagagtccgg cgagaaaaac aaatacggag aagaaggcgg gcaaatgcgc
    32341 gcagcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga
    32401 aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc
    32461 cacagtcggg tgtcgaactc aagctggtgg tactggggtt ccatatgaca acacaactgg
    32521 taaaacgctt tgttatgctc tttctccttc aggtgcgcca gctcgtgcac cacgatcatg
    32581 cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga cgcggatctc cacctttgct
    32641 ttcagcttgc cgccctgcac acgagaaaca gcggtatgta acccgagcgc gttttttagc
    32701 acatggatct tattgtcata catcacttta ttgatcggcg gggcattacg cagaaactga
    32761 tttttcagat cctgagtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc
    32821 ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagtgt tcgcacctgg
    32881 gaaagtagct gctcgggata accctgaaga taagtaagat tgctcataac cgcccacgaa
    32941 aaagagaaaa agggtatact cacgcaccct tttcaggggc aatgtcgaaa ttttatcatt
    33001 caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc
    33061 cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc
    33121 aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg
    33181 cgttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca tacatcagtg
    33241 aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc
    33301 tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa
    33361 cactggcatt gctggaacag caactgcgtg cgctgcgcaa agtggtcacg tcggatacac
    33421 gtattattgc cggtgccaag gcccgtgaca ttcacacttc cacgctggaa ctgttcgaaa
    33481 aagtgctcgg cccaaccacc accacgctgg catggaaaaa agcgcgcctg attaattgca
    33541 ctttcaatga accgcagctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta
    33601 ctgactggac tatccacaac catgcgaatg tcttctcccg caccgggctt gatatcggcg
    33661 cgcgcttctt tatgcaacat ctgccagaga atctcgaagg ggagattgtc gatctcggtt
    33721 gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa cccgcaggcg aaagtggtgt
    33781 ttgtcgatga atcgccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc
    33841 cagaggcgtt ggatcgctgc gagtttatga tcaacaacgc gctctccggc gtggagcctt
    33901 tccgctttaa tgctgtgctc tgcaacccgc cgtttcacca acaacatgcg ctgaccgata
    33961 acgtcgcctg ggagatgttc caccacgccc gccgctgcct gaaaatcaac ggcgagctgt
    34021 atatcgttgc caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact
    34081 gcaccactat tgcgacgaat aataaatttg tggtactgaa agcggtgaag ctgggacgtc
    34141 gtcggtaaga ttgggagtta cctgccggat gcggcgtaaa cgccttatcc ggcctacgtt
    34201 cagcacgtta ctccaggccg gataagacgc ggcaagcgtc gcatccggca atgtctgccc
    34261 agttctggaa tcgcgcttcc ataaaacaaa aaatccctgt caccatcgtc tacattctct
    34321 tgtttagcgt ttttctacgt ttattcttcc gtcacacaga taaattcatc cgttgccaat
    34381 ctggcaacgg atgttatcat attgccaatt tgtcaacgga cgtgatgaag aatgcacctg
    34441 ataactcaaa aagcattgaa agatgctgcg gaaaaatacc cgcaacataa aacggagttg
    34501 gtggctctgg ggaacacgat tgctaaggga tatttcaaaa aacctgagtc attaaaagca
    34561 gtattcccat ctctggataa cttcaaatat ctggataagc attatgtttt caatgttggg
    34621 ggcaatgaat tacgtgttgt agcaatggtc ttttttgaat cgcaaaagtg ctacatacgt
    34681 gaagttatga cgcataaaga atacgatttc tttaccgctg ttcatcgtac taaggggaaa
    34741 aaatgattgc gattgccgac atcttgcaag caggagaaaa gctaactgct gtggcacctt
    34801 ttctggcggg tattcagaac gaggaacaat acacccaggc gctggaactg gtagatcatc
    34861 tgctgctcaa cgatcctgaa aaccccttgc tggatctggt gtgtgccaaa ataaccgcgt
    34921 gggaagaatc agcgcccgaa tttgcggaat ttaatgccat ggctcaagcc atgcctggcg
    34981 gtatagccgt gattcgtacc cttatggatc aatatggttt aaccctttcc gatctgccgg
    35041 aaattggcag taaatctatg gtgtcacgcg ttttgagcgg gaagaggaaa ttaacgctgg
    35101 aacacgctaa aaaattggca acgcgattcg gcatttctcc cgccttgttt attgattaac
    35161 gcgacgtgcc ggatgcgttg catccggcgc ttcaaataac gatttaaatc tccagcgcca
    35221 gccgtgttcc ctgggcaatt gcccgtcgtg cgtccagctc catagccaca tcgcagccgc
    35281 caattaaatg caccgttttc ccgctatcaa tcagcggttg cgccagcgcg cggtttggct
    35341 cttgccctgc gcagatcacc acattgtcca ctgctaatac ctgcgtttcg ccgttgatca
    35401 ccacatgcag cccgtcatcg tcaatcttct gataacttac gcctgggatc attttcacac
    35461 cccgcgagag cagggtggtg cgatggatcc agccggtggt tttgcctaac ccctgtcctg
    35521 gtttgctggc tttgcgctgg agcatcacaa tctgccgtgg gctacggggg atctgcattc
    35581 cctgcgggct taagccacca gcctgttgta ggctactgtc gatcccccat tcattacaga
    35641 acccggcgat attctggctg gtggattcgc ccggctgact taaatacatc gccgtatcaa
    35701 aaccaatccc gccacaaccg atgatggcaa ctttgttgcc aaccggcgct ttgtcgcgca
    35761 gtacatcgag ataactcaat accttcggat gatcgatccc gtcgatgggc ggagtgcgcg
    35821 gcacgatccc actggcgagg atcgtttcat cgaaagcctg taactgatcc gccgtcacgg
    35881 tgtgattgag ttttagcgtc acgcccgtca cttcgatcat ccggcggtaa tagcgcagcg
    35941 tttcgtaaaa ctcctctttg ccggggatct gtttggcgat attaaactgc ccgccaatct
    36001 cgctatgagc gtcaaacaat gttacctgat gcccacgcgc cgccgcgtta atggcaaacg
    36061 ccagcccagc aggtcccgca ccgaccaccg ccagattttt tttctgcacg gcgggaagga
    36121 ttggcatttt ggtttcgtgg caggcgcgag gattcaccag gcacgaggtg actttgccaa
    36181 cgaagatttg atcgagacag gcctgattgc agccaataca agtgttgatc tcatcggctc
    36241 gtcccgattg cgcttttgac agcagctccg catcagcaag aaacggtcgc gccatcgata
    36301 ccatatcggc atcgccgcgc gagagaatat cgtcggcaac ctgcggatcg ttaatccggt
    36361 tggtggttac cagcggcagc gagacgtggc ctttcagttt gcgcgtgacc cagctaaatg
    36421 cgccgcgcgg cacgggcgtg gcaatggtcg gaatacgtgc ttcatgccag ccaatgccgg
    36481 tgttgataat ggtcgcgccc gccgcttcaa tggcctgcgc cagctctacc gtttcggcaa
    36541 aagtcccgcc gtcttcgacc aggtcgagca tcgacagtcg gtagataata atgaagtcgt
    36601 tgccgacgcg ttcgcgcacc gcacgcacta cttctacggc aaatcgcatc cggttgcggt
    36661 aatcgccgcc ccactggtca ctacgctgat tggtgcgcag cgtcagaaat tcgttgatca
    36721 aatacccttc ggaccccatt acctctacac cgtcgtatcc tgcctcccgt gccagttgcg
    36781 cgcagtgggc gaaatcgtcg atcagttgca ggatctcttc atgggtgagt tcgtgaggaa
    36841 cgaaacggtt gatgggggcc tgcaatgcgg agggagcgac cagatgcggt tggtagctgt
    36901 agcgcccggt atgcaaaatt tgcagggcta ttttgccgcc ttcctgatgt accgcttcgg
    36961 taatggtgcg atggtgtggg atctggctgg cgtcgttgag cattgcgcca ccttccatgc
    37021 caacgcctgt taaatctggt gcaatgccgc cgctgacaat cagcgccacg ccgtgacggg
    37081 cgcgttcggc ataaaacgct gccagccgct cggcaccgtc cgggtattcc tccagcccgg
    37141 tgtgcattga gcccatcaac acgcggtttt ttaacgtggt aaaacctaaa tccagcgggg
    37201 cgaacagcga cgggtagctc ataaaattgt ccagtatgta aaataattgt tatgtggtcg
    37261 gatgagttct aatttagccg tcgccggaga aaagggaaaa ggggagtgcg gggtttgtga
    37321 tgggattcaa aaagaatacg cccggtgata acgccggatg cgacgcttgc cgtgttttat
    37381 ccaggttacg gaattgcaca tgtagggcgg ataaggcgtc cacgccgcat ccgccagtgg
    37441 cgcggtgcag atgccggatg cgacgcttgt cgcgtcttat ccgacctacg ggaacgcaca
    37501 tgtagggcgg ataaggcgtt cacgccgcat ccgccagtgg cgcggtgcag ttgccggatg
    37561 caacgcttgc cgcgttttat ccggccagga ggattgcaca tgtagggcgg ataaggcgtt
    37621 tacgccgcat ccgccagcag ttgcaaatta ttgcttacgg aaaaaatcgt tatacaacat
    37681 atacaaatgc gcggcactcc aggagaaatt tggtgcgcct tgctgtgcgc ctgtcagcgg
    37741 gttgtaattt tcctgaattg gtccatcggc ggttaaccct ttggcgtgcc ggaagaacgt
    37801 atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt tcagaccaaa
    37861 ccagaactga tccacccata cgcgcccacg ccagtagata tcagcgccaa aagccggatt
    37921 ggttaacgcc gccgttccca gcgggacaaa ggtgttgaac tctttaggat cgagcatcac
    37981 cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg gcgaccagcc
    38041 ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg ccagtggttt
    38101 atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac aggtgttgat
    38161 gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg gcttaccgag
    38221 gatggtcgcc atctccgcca gataatggtt atcgctgtac atatagctgg cctgatccac
    38281 cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt tttcggcgaa
    38341 tttcaccgtc cagtcgctac gtttgccgcc attagcgaca tatttatcca actgttcttt
    38401 gtcgataaaa ccaaagacgg cggcgtcatc acggcccgat tcccacgatg cagcaacctg
    38461 tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt agttgttcag
    38521 cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct cgccgctctc
    38581 agtgttgtgg gctttgtcgc gggtcgcgcc atattccggc acgccgttgc cgttgtgatc
    38641 gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct ctgccagcca
    38701 ggttttatcc tgggtgacgt tatacacttc catcaccgac caggcggcaa ggctgggttt
    38761 ggtattacgt tcgttccagt tgccgccatc gccaccacgc tcggggctaa ggttccacgc
    38821 tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccgg gctggatctg
    38881 ccaggagaag accgcgcgga tattctcttt ggcgatgtcc ggattgaaat gcgccatcgc
    38941 aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc agcgcccggt
    39001 caccgacggc gtgacggtgt tgtatttcac cgcgccgcca ggcgagcgcc agttaccgtt
    39061 gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat ccggattggt
    39121 taaccctttc ttcagatact cttcccagcg ttgctgcgag gcggtgagat aaaacgccgg
    39181 acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag cggtcagcag
    39241 atgggaatag gtggtataga gcgtcgtcga accgttgata tgcgccttac tggtaaagcg
    39301 attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt ccgattcgcc
    39361 ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca ggccatcacg
    39421 ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt tatcggaaag
    39481 cggtttccct tctttcgctt ccagtttttc cagcagttcg ccgtcccaca ccagatccag
    39541 cggtttattg ctggtgattt tggtttccag tagtgacgtg cgcggcgtgg cgaagcgcag
    39601 agtcatttcg acttgcacat cttttgctgt cagtttttgc accagcgcgc cgggaatact
    39661 gtatgcctcc agcgtgaagt cgactttctt gccgtcctgc cagacggtca ggcggtcgaa
    39721 attgctggcc ataaagttga tgtactcttc cgtcagcagc gcaacgcctg gaaaaccgcc
    39781 catggtgtta gggccgtctg gcaacagatg accatgccag gctccgagat cgaaaaacgg
    39841 attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg cgccagtacg
    39901 gttaatcacg tttttataat tgtcggcgtt agcggcatgg gcggaaaaac ttatcagcag
    39961 agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt ccttattacc
    40021 agtagaaata ttgcatcagg aagatttggt cgctgggatc gtgagaggca tttttcatgt
    40081 agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc gtattcgtac cagataccaa
    40141 actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg gcgtaggtgt
    40201 aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc atcatcccac
    40261 tttcacttcc cggtacgtcc agcccgtcgg cgcgtccggt atggtgccag gcgcgagcgg
    40321 caaagttagg agttagcagg cccgtgagat agagctgtcc tgtgccgtcg gtgatttcca
    40381 gaccgttcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg ttcatcattg
    40441 ccggataggt gtatttgtcg tcatagcgat cgtacttgcc aaaatgtagc caggctttgc
    40501 tttcgtcatg atgtccggca ggtgtggcgg tgacgctata acgaaagttg ccgcttaagt
    40561 tctgcacctt cagcgcggtc ataatgtcgc gggtattggg aatgacgtag cccagagcgg
    40621 gggtgaagtc gccccaccat tgcaggtcat ccagcgagga atcctgacgc agactgagca
    40681 tgatttcggt gccatcattg gtacgataac cgccatagaa acggttaatt cctccctcga
    40741 aaccacccca gctatggtct ggcacccagg cattgccgtc gtggtcggcc tgcacggtcc
    40801 agccttcgcc gtaaatcagg ccgaaccagg agccgtactt catctccagg ccaccttcaa
    40861 tataggtgcc gtcgctgtat ttatgtttat tctcaccctc taaatccata tatccaccaa
    40921 cccctaa
//
LOCUS       DACAPB010000016        39799 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.016, whole genome shotgun sequence.
ACCESSION   DACAPB010000016 DACAPB010000000
VERSION     DACAPB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.016"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(205..606)
                     /gene="pemK"
                     /locus_tag="IT904_000776"
     CDS             complement(205..606)
                     /gene="pemK"
                     /locus_tag="IT904_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HAP3190603.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(539..796)
                     /gene="pemI"
                     /locus_tag="IT904_000777"
     CDS             complement(539..796)
                     /gene="pemI"
                     /locus_tag="IT904_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HAP3190604.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(889..1542)
                     /locus_tag="IT904_000778"
     CDS             complement(889..1542)
                     /locus_tag="IT904_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP3190605.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGAVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(2481..3338)
                     /gene="repA"
                     /locus_tag="IT904_000779"
     CDS             complement(2481..3338)
                     /gene="repA"
                     /locus_tag="IT904_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAP3190606.1"
                     /translation="MADLLQKYYSQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRASRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVVAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3331..3825)
                     /locus_tag="IT904_000780"
     CDS             complement(3331..3825)
                     /locus_tag="IT904_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190607.1"
                     /translation="MQDGVANHETHSVIAGGIESDLHIDSSKYPHPFCSLLQKRAHFD
                     SFKHLIFIRGLLAIAHGKRKNKISASMVSFYVSLVQVMCAHYIPDTNKVNLSVKELSV
                     CSGGSYTRVWRALKTLDNDLHLIAFDGGTIWFRPDMFETLRVGPDELVTARRRGNSVG
                     GGHG"
     gene            complement(3806..3880)
                     /locus_tag="IT904_000781"
     CDS             complement(3806..3880)
                     /locus_tag="IT904_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAP3190608.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCG"
     gene            complement(4112..4369)
                     /locus_tag="IT904_000782"
     CDS             complement(4112..4369)
                     /locus_tag="IT904_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAP3190609.1"
                     /translation="MSQTENAVTSSSGTKRTYRKGNPVPARERQRASLARRSNTHKAF
                     HAVIQARLKDRLSELADEEGITQAQMLEKLIESELKRRATS"
     gene            complement(4653..4802)
                     /locus_tag="IT904_000783"
     CDS             complement(4653..4802)
                     /locus_tag="IT904_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP3190610.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(5483..5695)
                     /locus_tag="IT904_000784"
     CDS             complement(5483..5695)
                     /locus_tag="IT904_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAP3190611.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(5831..6391)
                     /locus_tag="IT904_000785"
     CDS             complement(5831..6391)
                     /locus_tag="IT904_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAP3190612.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAGRLEKIRRQNRTKAELQAVLDGK"
     gene            complement(6494..7354)
                     /locus_tag="IT904_000786"
     CDS             complement(6494..7354)
                     /locus_tag="IT904_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP3190613.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYKGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(7413..8159)
                     /gene="traX"
                     /locus_tag="IT904_000787"
     CDS             complement(7413..8159)
                     /gene="traX"
                     /locus_tag="IT904_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAP3190614.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            complement(8179..13449)
                     /gene="traI"
                     /locus_tag="IT904_000788"
     CDS             complement(8179..13449)
                     /gene="traI"
                     /locus_tag="IT904_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAP3190615.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLT
                     LRDAQDETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QIRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPRTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            13531..13758
                     /gene="vapB"
                     /locus_tag="IT904_000789"
     CDS             13531..13758
                     /gene="vapB"
                     /locus_tag="IT904_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAP3190616.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            13758..14156
                     /gene="vapC"
                     /locus_tag="IT904_000790"
     CDS             13758..14156
                     /gene="vapC"
                     /locus_tag="IT904_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAP3190617.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(14165..16318)
                     /gene="traD"
                     /locus_tag="IT904_000791"
     CDS             complement(14165..16318)
                     /gene="traD"
                     /locus_tag="IT904_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAP3190618.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQKMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(16571..17302)
                     /gene="traT"
                     /locus_tag="IT904_000792"
     CDS             complement(16571..17302)
                     /gene="traT"
                     /locus_tag="IT904_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HAP3190619.1"
                     /translation="MKTKKLMMVTLVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(17334..17831)
                     /locus_tag="IT904_000793"
     CDS             complement(17334..17831)
                     /locus_tag="IT904_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HAP3190620.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLADWGNAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRDVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRQVSGCI
                     SIKKWGQNQLKDQYDGSEKLDFGGIEQTPTIYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            complement(17847..20669)
                     /gene="traG"
                     /locus_tag="IT904_000794"
     CDS             complement(17847..20669)
                     /gene="traG"
                     /locus_tag="IT904_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HAP3190621.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPLLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAVAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASYDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(20666..22042)
                     /locus_tag="IT904_000795"
     CDS             complement(20666..22042)
                     /locus_tag="IT904_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HAP3190622.1"
                     /translation="MMPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTT
                     QPGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQ
                     LQRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFVQKMASDINSMNLSSCQAAQGIIG
                     GLFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKN
                     INIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEG
                     GTAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKG
                     FISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNY
                     DEAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNN
                     YHFGGSTR"
     gene            complement(22039..22452)
                     /gene="trbF"
                     /locus_tag="IT904_000796"
     CDS             complement(22039..22452)
                     /gene="trbF"
                     /locus_tag="IT904_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HAP3190623.1"
                     /translation="MNHRKYQRELIMRENKSNPELKIRSTERDYKYISRITGRYAGLS
                     LVFLTAGIVLWTVMDIIFDACIDSWKADPELNNSSYMWNILIYAIPYALYALAAGFLV
                     TFFSVPNVRINIRKYRDIPAEMSYAPGEHIKGGQE"
     gene            complement(22406..22747)
                     /gene="trbJ"
                     /locus_tag="IT904_000797"
     CDS             complement(22406..22747)
                     /gene="trbJ"
                     /locus_tag="IT904_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HAP3190624.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIVTGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLSLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESS
                     EISEGTYNERK"
     gene            complement(22677..23222)
                     /gene="trbB"
                     /locus_tag="IT904_000798"
     CDS             complement(22677..23222)
                     /gene="trbB"
                     /locus_tag="IT904_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HAP3190625.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRGEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FSYTLDGQGDTAFPEALPVPPEVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARVDTVLQMYGGKKGAK"
     gene            complement(23209..23493)
                     /gene="traQ"
                     /locus_tag="IT904_000799"
     CDS             complement(23209..23493)
                     /gene="traQ"
                     /locus_tag="IT904_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HAP3190626.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            23574..23888
                     /locus_tag="IT904_000800"
     CDS             23574..23888
                     /locus_tag="IT904_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HAP3190627.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLPGNAAIIALIYAASHSLPVNAFP
                     DYLVISLLSIAAGIVVLWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDR
                     FI"
     gene            complement(23890..24237)
                     /gene="trbA"
                     /locus_tag="IT904_000801"
     CDS             complement(23890..24237)
                     /gene="trbA"
                     /locus_tag="IT904_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HAP3190628.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(24253..24996)
                     /gene="traF"
                     /locus_tag="IT904_000802"
     CDS             complement(24253..24996)
                     /gene="traF"
                     /locus_tag="IT904_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAP3190629.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVSAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     IRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(24989..25246)
                     /gene="trbE"
                     /locus_tag="IT904_000803"
     CDS             complement(24989..25246)
                     /gene="trbE"
                     /locus_tag="IT904_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAP3190630.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(25273..27081)
                     /gene="traN"
                     /locus_tag="IT904_000804"
     CDS             complement(25273..27081)
                     /gene="traN"
                     /locus_tag="IT904_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAP3190631.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKEQGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGGGVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(27078..27716)
                     /gene="trbC"
                     /locus_tag="IT904_000805"
     CDS             complement(27078..27716)
                     /gene="trbC"
                     /locus_tag="IT904_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAP3190632.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLETPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(27725..28717)
                     /locus_tag="IT904_000806"
     CDS             complement(27725..28717)
                     /locus_tag="IT904_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TraU"
                     /protein_id="HAP3190633.1"
                     /translation="MKRRLWLLMLFLFAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(28714..29346)
                     /gene="traW"
                     /locus_tag="IT904_000807"
     CDS             complement(28714..29346)
                     /gene="traW"
                     /locus_tag="IT904_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAP3190634.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(29343..29729)
                     /gene="trbI"
                     /locus_tag="IT904_000808"
     CDS             complement(29343..29729)
                     /gene="trbI"
                     /locus_tag="IT904_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAP3190635.1"
                     /translation="MSSTQKPADVMAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDRASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(29726..32353)
                     /gene="traC"
                     /locus_tag="IT904_000809"
     CDS             complement(29726..32353)
                     /gene="traC"
                     /locus_tag="IT904_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAP3190636.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(32513..32734)
                     /gene="traR"
                     /locus_tag="IT904_000810"
     CDS             complement(32513..32734)
                     /gene="traR"
                     /locus_tag="IT904_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAP3190637.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(32869..33384)
                     /gene="traV"
                     /locus_tag="IT904_000811"
     CDS             complement(32869..33384)
                     /gene="traV"
                     /locus_tag="IT904_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAP3190638.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(33381..33632)
                     /locus_tag="IT904_000812"
     CDS             complement(33381..33632)
                     /locus_tag="IT904_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HAP3190639.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(33644..33874)
                     /locus_tag="IT904_000813"
     CDS             complement(33644..33874)
                     /locus_tag="IT904_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HAP3190640.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVLSNCAT
                     RIVLPAPEKFGSESLPDNFNMTAVGVMKNSEI"
     gene            complement(33828..34418)
                     /gene="traP"
                     /locus_tag="IT904_000814"
     CDS             complement(33828..34418)
                     /gene="traP"
                     /locus_tag="IT904_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAP3190641.1"
                     /translation="MANNTSSRQAGHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(34408..35835)
                     /locus_tag="IT904_000815"
     CDS             complement(34408..35835)
                     /locus_tag="IT904_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TraB"
                     /protein_id="HAP3190642.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(35835..36539)
                     /gene="traK"
                     /locus_tag="IT904_000816"
     CDS             complement(35835..36539)
                     /gene="traK"
                     /locus_tag="IT904_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAP3190643.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(36550..37116)
                     /gene="traE"
                     /locus_tag="IT904_000817"
     CDS             complement(36550..37116)
                     /gene="traE"
                     /locus_tag="IT904_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAP3190644.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(37138..37449)
                     /gene="traL"
                     /locus_tag="IT904_000818"
     CDS             complement(37138..37449)
                     /gene="traL"
                     /locus_tag="IT904_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAP3190645.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(37464..37829)
                     /gene="traA"
                     /locus_tag="IT904_000819"
     CDS             complement(37464..37829)
                     /gene="traA"
                     /locus_tag="IT904_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAP3190646.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(37862..38257)
                     /gene="traY"
                     /locus_tag="IT904_000820"
     CDS             complement(37862..38257)
                     /gene="traY"
                     /locus_tag="IT904_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAP3190647.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            complement(38356..39045)
                     /gene="traJ"
                     /locus_tag="IT904_000821"
     CDS             complement(38356..39045)
                     /gene="traJ"
                     /locus_tag="IT904_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HAP3190648.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKKFIYYL
                     YEKAMCIINTR"
     gene            complement(39232..39615)
                     /gene="traM"
                     /locus_tag="IT904_000822"
     CDS             complement(39232..39615)
                     /gene="traM"
                     /locus_tag="IT904_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAP3190649.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
BASE COUNT         9367 a        11164 c         9349 g         9919 t
ORIGIN      
        1 gcagcatcac cggaacttca aaactgcgca aaacacgcaa atacgcacaa gaaagtaggg
       61 ctgcgcttgg aattcggaaa ataaaccaca tcaaaactac tttttagcca aataacgttt
      121 gggaatcacc agaatggtgg gacaacagcg gttttagtgc cctaaatcgt acgttttcat
      181 ccagttgccc ctcaaacccc atgttcaagt cagaatagtg gacaggcggc caagaacttc
      241 gttcatgata gtctccggaa cccgttcgag tcgttttccg ccccgtgctt tcatatcaat
      301 tgtccggggt tgatcgcaac gtacaacacc tgtggtacgt atgccaacac catccaacga
      361 caccgcaaag ccggcagtgc gggcaaaatt gcctccgctg gttacgggca caacaacagg
      421 caggcgggtc acgcgattaa aggccgccgg tgtgacaatc agcaccggcc gcgttccctg
      481 ctgctcatga cctgcggtag gatcaagcga gacaagccag atttcccctc tttccatgtc
      541 agatttcctc ctgaccagtc gccggtgcat ccagccattc tcgttcttca gctgatattt
      601 cagcattcgg atcacactgt gccagtagct cagccagtga atattgcggg cgtctgtacg
      661 gctcaacaat cagccggcca ttatcaatga ccatgccaac ttcattatct gtgcccagag
      721 acagcgcatt cagcagtgcc ggtgggacgg tcagcataac tgagccgcca accctcttca
      781 gtcgggtggt atgcattctt cacctccata aaagttatat ttaaatataa catccactaa
      841 aaaaacacac caggctttaa cgcacaatgt ttaataaaaa tataactttc aaccaaacag
      901 taaacccagc gtggttgctt ccatatgcag caataccggc agcagcaggc caccacttct
      961 gatcctggcc actgatgtaa tcaaccccac caggaacagt tctgccagtg tcagcaggtt
     1021 ctgatactgg ctgtgcgcgg cgacgaacaa caacgacgtt atcagcgccc ccagccacat
     1081 cgtccagcag taccgtgaac ggaagacgtt cagcataatc ccccggaaca gcgtttcctc
     1141 attcaacggg gcaaggataa agatggtcag caacgtcagg atcacgtcag gtatggactt
     1201 atcggcaaaa agtttcgtca taaatggctc agcaggcaga gccagcgcct taccgagcag
     1261 aaatacaccg acatacacca cggccatcgc accgaccagc cacggtacgc caacgttgcg
     1321 cagctgacca acgaccggta gcggagctat ccaacggcgg tataccagga aaacacacag
     1381 caggtacatc agaacagcac catgactgaa gaacaaatag ttttttcctg atccataaag
     1441 cagaacggcc tgctccatga caaatctggc tccccaacta atgccccatg cagccagcat
     1501 aaccagcata aactgcagat attgattacg tgtttgaatc attgcatcgc ctgtaaattt
     1561 ttaacttgtc ctatttttgt cattaccacg tatatacaca tgtataacaa ttcagatatc
     1621 gttaccagga tatgccgcat cagcggcatg gaaggcggca ctctgttgtt tcatatgata
     1681 caggagtaaa accgccgaag cccggcgtaa gccggtactg attgatagat ttcaccttac
     1741 ccatccccag ccctgccaga ccatacccgc tttcagccat gagagagctt ctgtgcgcgg
     1801 tcggagtggt cccgacgagg gtttacccga agtcggggcg tatctccgcg ttagcgggcc
     1861 gtgagggccg cttacgagcg tgtactgaga acttccagcg agaagactga cagcgatgaa
     1921 gatgtagtta caacattcat aattaaaagc gactctgttc cggccctttg ggccggggcg
     1981 gggccgcttt tcagttatga gggaggggct ttgtggtttc agttctgcgc tggttcgggg
     2041 tttttctgga ggttggtttt gtgtgttgta actaaagtgg ctccggttgg ggcccgccgt
     2101 ttacggtggg aggtgcatat ctgtctgtcc acaggacagg cagtgaatag gttttctttt
     2161 taaatgaatg taattaagta gtttaaagga gatataaaca ggtgtttaaa agatacattg
     2221 caccctgtaa ggctggcgcc tggcgcttta tgacatgaac ggttgtaacc ttatggggaa
     2281 gtccattgca gttaaatgtg gataagcaaa attccccgtc tgtgaggcgt gttttgtatc
     2341 aaaaacaggg ggggccagat gcaccagaag gtggatgatg aggttgtttt ttgtatgagg
     2401 tgctgctttt ttgttcgctg gcaggcttca ggcgtgcgaa tgcctccggt gcgggccgga
     2461 ttattcagaa gaggtcactt tcagggggaa gctgtggcca gccggctgta attacggtta
     2521 cgtgacagaa tcatgcgctc tttcacacga cgctccactt cgcgttttac cgcctcacga
     2581 ttggcagtga agcgcccttc cgagatttca cgcgtcagct gccgtttcac cagggtgacg
     2641 atatcctgac gttccctgtt cgcatcacga cgcgcacggg cacgctttat tccacgggac
     2701 ttaagctctg tctggtaact gcggaaacgc tcacgcacaa aacgccaggc tttcgctatc
     2761 agttcatcca tacccagggt atccagcccc tgctttttgc gctgtctgtt ttcccattca
     2821 acacgactgc ggcgcgcagc aaccactgca tcctcagaca catcaagggc ggcaaataac
     2881 gccggtgtga acgtgatatc ggtcggaatg tagcacccga taagcggatc atattccgtc
     2941 tggtaggtaa tcagtcccag ctctgcaagg aacgtcaagg ctcgggaggc ccgggtgatg
     3001 gagagttttc cggcagcaga ctccgtcgcc agtccgcact caatggccag cgtagtgatg
     3061 gagcactgga cgcggttggc cagcgggtca tagtgaaaac acagcccctg cagcagcgca
     3121 tcaatagccc ggcgacgcag taccggtggc atgcgccgac gcagtccctt cgaacgggca
     3181 tgtgccacat gaatggcgaa atcaaaacgg gaggtgaagc ccaccgcctt ttccatcagt
     3241 ttttcgcaga acttcagcgt tccggcacct tcacggggtg tgaacaccgg attcggattt
     3301 ttaacctgtg aatagtattt ttgaaggaga tcagccatgt ccccctccaa cactattccc
     3361 cctcctacgg gcggtaacta gctcgtctgg gccgacacgt aaagtttcga acatatctgg
     3421 tctgaaccag atggttcctc catcaaaagc gatgagatga agatcattat caagagtttt
     3481 gagggctctc caaacacgag tgtatgaacc tccactacaa actgaaagct ctttaacaga
     3541 taagttaact ttgttcgtat cagggatgta gtgtgcgcac attacttgta cgagggaaac
     3601 ataaaaacta accatcgacg cgctgatttt atttttacgt ttgccatgcg ctattgcaag
     3661 caaacctctg atgaagatca gatgcttgaa ggagtcaaaa tgagcccttt tctggagaag
     3721 gctgcaaaac gggtgaggat acttgctgga atcaatgtga agatctgatt caatcccacc
     3781 cgctattact gaatgtgttt catgattagc cacaccatcc tgcacttaca atgcacagaa
     3841 ggagtgagca cagaaagaag tcttgaactt ttccgggcat ataactatac tccccgcata
     3901 gctgaattgt tggctatgcg gttttatggg gccccggtaa tctttctagt cgccaaactt
     3961 gaagaagatt atcggggttt tcgcttttct ggctcctgta aatccacatc agaaccagtt
     4021 ccctgccacc ttacggcgtg gccagccaca aaattcctta aacgatcagc aatctaccac
     4081 tcactcctga gataagcaag aatgtgaata tttacgaagt cgctctgcgt ttcagctctg
     4141 attcaatcag tttttcaagc atctgcgcct gagtaatacc ttcctcatcc gccagttcac
     4201 tcagcctgtc ttttaaccgg gcctgaataa ccgcatgaaa agccttatga gtgttgcttc
     4261 tgcgagccag agaagctctt tgcctctctc tggccggaac agggttacct ttccggtatg
     4321 tacgcttggt gcctgatgag gaagtcactg cattttctgt ctgcgacatc tcgcctcctc
     4381 aattctcaaa cagggatcgt ttcgcagaga atactacagt tttttgaaat cagcgacttg
     4441 aaagttgtga cgatgatctg tgttggcgca caaatcatgt ctgattacga ccgtttaatg
     4501 tgattttgat aatgaaatga ctgaaaagct ggaaatgcct tagccagggt gccggccccg
     4561 ggcgggaccg gctgtttctg gtatataggg tgtctgcccc tgaaatacct cacacttccg
     4621 gaaagcgggg acttccgtcc ccgcccggca gcttacttcc gtgcttcgta ggccagagtt
     4681 acctgcacca ctgtatttcc tctgtgaata ttcagctcac ataaccgttc cctgaatatc
     4741 agtgaaaaac acaacaccgt ggcgcacacg gcgagcaacc cgataagggc atatttcgtc
     4801 ataaactttg acctctcagc aaagaagagg ctacaatcag tagtgttaag gtacttcatg
     4861 cagacctcac gagttaatgg attaacgagt ggggtcttcg catttctggt acctgcgtct
     4921 tgcctgaacc agaaaggctc aaacacccgc cgcgataata ccacattccc tgttgctgac
     4981 tattaccccc atttgcctgt ttttgttaca aaaacgccgg gagcggggga aaacttactt
     5041 tttcccgttt ccggggggga caactgagca ttgcgaacgc gtcagctacg atgttccggt
     5101 gattgctgat cagcccctgt tgtattcata aactaattaa cattcattct gtctggtgca
     5161 taaatctgcc cgtgaacatc tgcagacaat accggccatg aaaagataaa taataccagt
     5221 ggaatatatt tcctcatgtt attctccata tcagataaat ggcacctgta tttcagcccc
     5281 cctgaatatc gcagcacaca ttgaataaga ccttattttg tgaaattaat cacaaaatat
     5341 aaaatcgcga cttaatacgg aaaaaactct gtgctactca gaactggccg gtcatactgc
     5401 ccgaccggat attaaaaaaa gcaaatctca taaactcctt tcagccttct gactcctgga
     5461 gtaatgaagg ctgctttttt cgttacaccc ttccccggca gcgccggtgg cagtgttcag
     5521 cccttttccg ggcaaactgt tgccagtctg tccggggagc tatgcaggca gcccgtcgcc
     5581 atgcttctgc cgcttccatc cagcgtccac tgacctccag aataacggcc cgcccgccgg
     5641 cacgctggta acgccagacc tgaccgttcc gtgaactgtt tctgaatcca ttcatttttc
     5701 tgctcctccc ttttagtcgt cagtattatt gtggtaaata attcagtgga ctggcttcct
     5761 ttcgggtacg gaagttattt ccttttaagc cttattgttt cagcctcccc ggtttccggg
     5821 gaggcttttt ttatttccca tcaagcacgg cctgaagttc tgcctttgtc cggttctggc
     5881 ggcgaatttt ctccaaacgc cctgccgcat acgcttcctc ctcctgagaa atatgctccg
     5941 tcacataccc ttccgtgtca taccggcagg caccggcttt catggcacac agatagcttt
     6001 ctgaacgggt gatggccttc agcgcccggc gcagtttttt atgcgagagc gggatattcc
     6061 gctgcgccac gtcttccagt aacacgtccc ggataccgca ggccagaagc cggggcgtgt
     6121 taccgtcaaa caatcccggc caccagggtt tcagagtgtt caccgcctca tccagcgagg
     6181 gcagcgtcag ataaatggac agcgcctgtc tggcctgtgc tttcttcgcc gccagctctg
     6241 cttcccgggc agccttctcc gccagtttct gctttttcac cttccatttt ggtggcgtgg
     6301 tgacattgat gatggttttc cggctccggg tcggtgtttc cccttccgtt ttccgtttca
     6361 gtgtcagtac cggtcgtttc tgctctgtca tgattgccct gacctttgtg aaaatatgtc
     6421 ctccaggtcg ttgaaactac tcactatgat attaagctga cctcaacgac ctctgacata
     6481 ttaataccga caattaaaga tgttttttaa accattgcgt ctgtactgcc gccacacgtt
     6541 caaaaaacgc gcctttataa atatcataat ggcatgcgtc agcttcctcg tataattcct
     6601 tagtaccaga agcaacagca tcatataagg ctcttccctg ttctggtgga ttaacactat
     6661 cctggccggc aataacaacc aaaactgggc actgtacttt ggctgcgctc tctgccggtt
     6721 tatattgcaa cgtctccata accgtaagga aaggtatttt tatatccatt tcaggatact
     6781 gaccttttac cttctcgaag aatacctttg attcattatc actcagtacc cgggtaaccc
     6841 ccacaaacat ctctttcccg gtatttttct tcttttctgc cattttattc agcgtggaca
     6901 gaaaagaagc tttctccagt tcattcattt ctccggtaac cagaacctca ccgtcagcaa
     6961 aagccagctg gctgacaata cattttacgc gttggtcctg cgcagctgca ctgaagacat
     7021 gcccccctcc cagcgatgtt ccccataacc caatacgctg attatcaata catacctgct
     7081 tttctgccca attgataact gaaataatat cttcggtctg cattgccgga accagacgac
     7141 ctcgttcccc ttcactctca ccgaatccac gataatcaaa agtaatcgtt gcaaacccgg
     7201 cttctgtaaa cgcattcgca aaggaaggca gcagaacatt ccggataccg caaaatccat
     7261 ggcataagag aatcaaagga tgttttatat ttccttccgg gacacgaaat gtcagggcaa
     7321 taccttcagg gagtttgtga tccgtaattt tcataaatat cctgtgttat gttgtttatt
     7381 aataagcaga atcagcgcca gcatcatacc agtcacacag ccagaacgcc cagcacagta
     7441 agatgacagg catagaacgt cgggaaaaaa tccccgtacc agaaacgggg tagtgatttc
     7501 cctgtacagg acaccagacc aacagtcagc accgtcatca caaggccggc caccgccgct
     7561 gcatcactgg tggcaaggtt aagcgccgga ataacagcca gcaggcaggc gaccagcgcc
     7621 agacgttcca ttctgtcttc cgcccggtac agcctgtggc tgactgccag catcagcagc
     7681 ccggcaatgc cgtaactggt gccggagaaa ggcaaccaca ttgccagaag caacatggtc
     7741 tctgcggaac gccaccaggt gcgcgtttca caccatgtca gcacctgtgc cgcaactgca
     7801 aaggcaaaca ggatattccc ctcataccag ggaaagccgg ccagataata tgcgaactgg
     7861 gcaatcaccg cccatcccca cagccggtta atggcctcct gccggatatg cgtatgacgg
     7921 gacagattca gcccccacac cagggcaaac agcggaaagg ccccacgccc gaccagaaac
     7981 atccacgact ggtccagatg cagaatgcgg tttgcatggt ccagaaccat cagcaccagt
     8041 gccaccgttt tgatgatatc ccgctgtccg ggtgaccaga ccagcaacga ctgcaaccag
     8101 gtatcggtcc gggcagccag tgaatcatta cgtgttgtgt tcgtgttatc cgttgtcatc
     8161 agcgtttatc ttcctgtgtc agtctccgcc cagggttttc tctttctgca ggtcacgaac
     8221 catatcccgc tccatctgct gcagtcgctc ctgcagcaga ttttcccggg caacctcccg
     8281 gaccgcttcc cgcacccgtt gtggttcacg cagtacactc tccggcagcg cggcttcccg
     8341 ttcagatatg gcagaccggt cacgctccag cccggcaata tccctgacag cctgctctgt
     8401 tttgccgtcc ggcaggtcag gtttgttctc tgccatttta cggacaattt catccgcacg
     8461 gcgttccgtt tcacggcgga tggcctcttc agcctgccgc tgtgccagta cctctgccgt
     8521 gaccggttcg ccatttccgg ctcccggctg aacgctgttg tccgggatat ccccccacac
     8581 gcgaccaccg gtgatggttc tgggattcca cggacgacct tcaccggcga ttcttaccac
     8641 cacaccgctg tcaggattat cacgggcaat ccggacacca tcctgcatat tatcagccag
     8701 caggctctct ccgttacggc tgccctgcag ggccacgaac tgcgcatccc cgctgccttt
     8761 cacccggcct tcaccactga agccccgcag cccgtttccg tcatccgtgg tcagcgggtt
     8821 cagccagata ccagctgact tgccgttacg gtcaaaagcc ggcagtgcca catacggctg
     8881 cggatatttg cgcccgggtg caataaaccg tgcaggactg tctcccccgg ccagccctgc
     8941 ctgacggaga acggcacggc cggccaccac gtcccgcagc tcccgcgccg tactgaacag
     9001 ccgctctgca ttcatgacct cccggtccga tttcggctct agcacatcgt gggcagttcc
     9061 tttctgtacg gcattgttga tggcatccgt ccagccctga cggttatcgg tgtacacctg
     9121 cacatgctgc ttcatacgcg acagggccac gtaggctgac tcaaagccgg ccatcagttt
     9181 ccggttacct tccgtgcctt caagcgcgat ggcaaaggtt tcacttgccc cctgcgcacc
     9241 gtgggcggtg atggcataag ccaggtcaat atgttgctct gcccgctcct gacctgggcg
     9301 aatcacccgg atctgctgtc cgtccgacag cgtgacactg tcaccggaaa ccgctgtcac
     9361 cgtccagacg ctgttggcca cataacctcg ctcccggtca ctcttcgtga agcgcatccg
     9421 gtcaccggtt cccacccgga tggtgtccgg ggtgtacagt gtgacgcctt cagccaccgc
     9481 ctcccggggc gaaatcagcc gcgtgttacc ttccgcatcc tccagggtta tcagcccgtc
     9541 atccttgctg ataccggcaa tccggtgata cacactatcc accagggcaa gggcatccgg
     9601 gttattctcc caggtggaga gacgacgcag ttctccgtca cgtatattcg ctgtgttcag
     9661 gacaggcacc atgacctgct ctttgcccag ctcaccggcc ttttcccgtg catcatgaat
     9721 catgctgttc agtacgcgcc ggtcctcatt caggtgtgtg acaatcagcg tctgctcccg
     9781 tgcttccggt gtcctgccgg tatagtcgcg gacaatggct tcatacagtg tcatggggat
     9841 atccgggaaa gcctcgcctt tcagcatcgc cttctgctgc gcttctgcca gtttcgcttc
     9901 ctggctgtga ctgaactccg tcacggagtg ctccggtgcc catgcgccct cctgacgggg
     9961 aacctgagac ggtttcacac tctcaagccc ggacagtgcc ctttccacat cccggttaat
    10021 caggctgtat accgcctccc gcagttccgg cgtctgacgc acaatctcct tcatgatgac
    10081 cacatcggca gcactgcgcg tctgctggag acggaaaggc tgaccgggcg cgatggcctg
    10141 caactggtcc gtgtcaccac tggccacggc acgaccgcca ccggccgcaa tcagggcgta
    10201 tgcccgtgcc atgtcggtat tgcccaccat agagctctca tcgagcagga acagcgtgtt
    10261 gctgaaattc ggcgtttctc cgctgcgctg cagcagctgc gtgtcatgca gaaaggacgc
    10321 cagcgtctgt gcatccacgc cggcgctgcg catctcaccg accgcacggt gcgtgggccc
    10381 cagccccacg acacggggac gttcactctc cggcagcatg ttcacggctg acatcaccgc
    10441 ccggaactgt gtggtcttac ccacaccggc atagccctgc accaccgtga aacggtcgga
    10501 cgtttccagt atcatgcggg tagcggcacg ctgtcctgat gtcagtttct ccatgagttc
    10561 gccaggtact ctctccatca gtggcgtgac cgcctcctta ccttcgagaa tatggcgaag
    10621 aatgctcttt tctgcctcat acgacgcacg ggaaaccagc aggcctgtgc catagccttt
    10681 tgccacatcc acatacagta aatcaccgcg ttttatctgc gcattgattt cccctcccag
    10741 ttcagtaaaa ctggttcctt ctgcagcaaa cgacttcgcc tctgtcagca ggtccaccat
    10801 gctgaaggcc aggtttttac tttccaccac aggaagcgcc agatgaatag cctgctgtgc
    10861 cggcgtgtga agcccggttt tctgcagact gatagcggtt tccagcaatg tctcaccggc
    10921 acgcgccttt atctgctcag aaaccaccgt aaaggaggga tggcgggcaa gtttttccgc
    10981 agtacgggtt tcatccagtg aggaatacag ccggacatca cgaccgctgc gggccagacc
    11041 gttcagggtg gcgttgtcca ttgccatctg tgtgacggag gcaaaaacct tcgcactgtc
    11101 gctgacggaa tgcccgggcg tttccaccca gccgttctcc agcttcagtg ccatgaatgg
    11161 tgaatcgctc acaggcaggg aggccggttc agcccgcccc ggcacaacaa ccgtcatcgc
    11221 atcttcactg acggatgcca cctgcaggcg gtcaccgccg gagacgcgga gtccgggaat
    11281 tttccctgtc accctcagtc gctcgccgtc tgccaccggc attttttccg gccggaacag
    11341 cgaccagctg ctgtccaggg aggaaatacg caccacctgc gtttcatcct gcgcatcccg
    11401 cagggtcagg ctgttactct gcgccgtcac ccggtcgata acatagcggt catgactgcg
    11461 tgtctccggg ttccactgct ccatcaccat ccccggacgg tacatatccc gcagataacg
    11521 gctccggctg tccagccaga ccggcgacag ggcggtcatg gttacctccg ggcgtccgag
    11581 cacgccctgt gttttcagct cactgcgaat ggcctgtgtc agtatggcct gttcccgtac
    11641 cccgctgacc tgtgccacgc tctcttctcc ggctttcacg ctggcagcaa aatctcctgc
    11701 cagccgggca tagcggacat tacggtccgg ttcactgatg atggtggccg gccgctgttc
    11761 tcccccctgc cagcgatatg tgttcacccc ggcatctttc atggccatca gtgcactgcc
    11821 tgtaccggtt cgctgcccgc tgtcggttat caggacctgt acgttatgac gtgcggcacc
    11881 gtccagcagg gttaacgtct ctttcaggga gagtttttcg ccctggtcaa cgataacagt
    11941 attgcccggc gtgaaggcca tgccctcctg cagctgacga cgtccggtta tcagctcacc
    12001 ggacagccgt tcatcctgct tcaggttcat ctgcgagcga cggtcagcgg cgataatctg
    12061 cacctcccgc ccctgctccc gggccatcat gaccagctcc gccacccgct cacgctgccc
    12121 ggctgcaccg ccctgcccgg acacaatagc cagcgacggg cgatcctgtg ccagaacact
    12181 gaccgcatcg ctgtaaccgg ccgtccgggg gacacttttc tccggatgta cggtcacccg
    12241 gttctgtttc atgatgtcac gactgagtgc ccggactgac agctcatcga gcacatgaat
    12301 cccggacgtg aacagcccct tctcacgatc gagggggata agctgctcac ggctgatggc
    12361 ctcatcgata ccggcgcgtg cccgttcaat cacaccattt tccggcggca gtatgccgac
    12421 cgttctggcc agcacgtccg tgtacgtaaa ctgcactttg cgttcactta atccggcaat
    12481 ggcctgtgtc accgcctgct gcacatccgg cccgtcctgt gaagcgggtc cgggcgtctg
    12541 cgtgcgggtc tccgcccgtt ggtccgccgc gtcacgatac gcccggatgt cgaacccggt
    12601 ttccttcagc gtctgcatcc attcagccat tctgacttca ggatcgacgt gctgtttgga
    12661 ttttcgcgta tccagtgccg ccacatcccg ggatttcagc gaggcgtctt ccccgacggc
    12721 ctcccggata gcctgtgagc gtccggagaa ggcctccacc ggtacgcccg gcatctccca
    12781 cataccgtgc ttaccaacca cttccgtttc atagcctagc gcctcaacct gctctttcag
    12841 tttttcccgg tagagcctgc cgaaggcaat ctgattagcg tacacattct caatgaaccc
    12901 cgttttcccc actttgtcac tgctcaatgt cttccactcg ccgttatgct gcgtgacatt
    12961 ggccactacc gcatgtgtgt gtaactgtgg ttcctgatcg cgactggtgt cgtggttaaa
    13021 cagtgccatc accagattac cggtcagcac cgtttctgac tgtccgtccg tcataacccg
    13081 tgtggaagcc agcgcctcca cctgacggac agcaaaatcc acggcctggt tatgtgcatc
    13141 aataagacgt ttatcaccgc ctaacatggc catcacggag acacttttgg gggcggagaa
    13201 ggtcaggtcg tagccgggac gatgcttatt actgccatcc tgcatgcggc ttaaatccgc
    13261 tccgtccggc agcttcccct ccagaagacg ggtaaaaaca tccttatcga cgctgccctg
    13321 cagccccagc tgttcagccc ccctgccggc ccagcgttct cccatgctgc ccagcacata
    13381 gtaattatcc ttgtcggtat aatagttacc ggcacttccg gccgatctga cctgcgcgat
    13441 actcatcata aacgtatatc cctttgacat atcccggtat caatcccaca atggatatac
    13501 acaagacata tccacataag gaggcaaata atggaaacca ccgtattttt cagcaaccgc
    13561 agccaggcgg tcagactgcc aaaagcggtt gcattgccgg aaaacgtaaa acgcgttgaa
    13621 gtgattgctg tcggacgaac cagaatcatt acgccagccg gagagacatg ggacgaatgg
    13681 ttcgacggac acaacgtcag cgccgatttt atggataaca gggaacagcc cggcatgcag
    13741 gaaagggagt cattctgatg ctgaagttta tgctcgatac caacatctgc attttcacga
    13801 taaagaacaa acccgccagc gtcagggagc gttttaacct gaaccagggg agaatgtgta
    13861 tcagttcggt caccctgatg gagctgatat atggtgcaga aaaaagccag atgcctgaac
    13921 gtaatctcgc tgtgatcgag ggatttgttt cccgcattga tgttctggat tacgacgctg
    13981 ccgcagccac acacagcggc cagataagag cagaacttgc ccgtcaggga cgccctgtcg
    14041 ggccatttga tcaaatgatc gcaggtcatg cccgcagtcg gggactgatt attgtgacta
    14101 ataacacccg ggaatttgaa cgtgtggacg gtctgagaat tgaagactgg agctgacctg
    14161 ttcatcagaa atcatctccc ggctcaacat cctccccgcg ctcccggtgc acattgatgt
    14221 tcacctcttc acgacgctgc atcttctgct ggatatccgg attctgctcc cgttgccatg
    14281 cctcataaac ggccatatcc accacttcac cggattcact gatcccgggt ggcagttgct
    14341 gttccatctc ttcttccggt ttcattttca gctcctgctc gatcccccct gccggaacat
    14401 tcacacctgc atctgacttc ttatcgttga tggcaggaga caccggctgt tgcggctgtt
    14461 cagcctgagt cacgtcttct ccggaaacaa cctccgggac atccggttcg aagaggctgg
    14521 ccatctgacg accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt
    14581 ccgggttgat gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca
    14641 gggagagttt tactgccgga tacggtccgg gcagggtgac ataacaggtc agatccggca
    14701 gagactgaat gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata
    14761 ccccgtcacg taccggatca gcaccgtagg aatactgctc gctggctttc aggtgctctt
    14821 tctcaccaat ttcaccggca gcgaactctg caatcttatg gctgggagaa cggaaaaagg
    14881 cacgggtgtt catgacgtca aacagcgtgg cagccgcttt ctcaccgtag atatcttcca
    14941 gctgggcata ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca
    15001 ggatctccac cagatccggc agtttgtgta acgtgggtaa ctcgtcacag aaaaaccaca
    15061 cacgacggtt acggttttct cccattgcca gcagaccacg aatagcaatg gacagccaca
    15121 tggagatcac cggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc
    15181 cgtttttctg atcttcccgg acaccacgca tccagtcacg gatggtgaag ggctcaccgt
    15241 tatgctcaat cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga
    15301 tggatatggc cgttttctcg attttctctt ccaccaggtt ggccgccggt gaattacgca
    15361 ggaaggtacg cagcttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc
    15421 tgcggttggg gtcattacgc atcaggtatg ccgcttccgc aaaaatggta cgtcctgaac
    15481 cctgccagaa cgggtcctct tttgtcccca tcgggatcag agtatttgcg gtattatcaa
    15541 aatctggctg tgtcaggcac tccttccaca aatcccaggc agcacagcgt gcatccagcg
    15601 gattcaggat tttatcgatg gagggatcat agtaactttt aacaaattcc cccgaacggt
    15661 cataaatcac caccatatca ccacgctgac gggcgtagtt ggccagacga cggataacct
    15721 ccgacttacc ggccccgaca gtgccgtgca gacagaagtt ctggatttca gaatcccgga
    15781 taatcggcag atcgccaatc cggatatcgg aatccttgcc atcttttttc agcatcctgg
    15841 ccacctcttt cggattgtca gtcagctgac gaccacctgt gatttcattt tcgctctgtt
    15901 gtttaccctg acgccccaga atccaggaga caacaaagaa ggtgatcagg cagataacca
    15961 gggccacgac agacgccaga acgaatgccg accataactg ctcaccgcac cagatcatat
    16021 atttatcatg cagtacctga gccgcgttca tccggaaggt tttgccgtaa tactggatct
    16081 catagaccgg ctgggacctg attaaatccc gcatgccttc cagcgtggtg caccaccagt
    16141 aaatgcagcc attcacaaac gtctgccagc ttatttttac ccataaaacc agaccaacta
    16201 ggatccagaa aaaaataaac aggcagtaaa gtatgatatt ggcgatctga ctgaacatgc
    16261 ggatacgcat ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga
    16321 tgatattctg aagaaagaaa gtcagtcgga ctgattatta atgatgaaaa gattaataac
    16381 cgttaaattc cggcttcgcc ggcaacactt cacggtcgca caaagaattt gcacaaccgc
    16441 tcagtgtttt catatttatt tcggaaacgc cggggagaaa taaaatttat tttatttctc
    16501 cccggggttg gacaacgagc aaagcgaggc gtcagtcagg aggccggtca gaccagcctc
    16561 cggaagataa tcagagaata tttgcgattg atttggccag ctggtcttcg agaacaggct
    16621 tcgcctcttc aaatttcagg ttaaccttgt tcgcatttga gaccacacgg gtctggtatt
    16681 tatgctggtt acctgtttca gtactggtct gaattttcgc acctgatgtg ccctgacgca
    16741 gggcggcaac attatccgtt gtcaccgttg ccttagtacg ctctgcaatc tgcacatccg
    16801 tgatcatggt atagttcaca tcttccacca tcgcatctgc agccataccc accagaccgg
    16861 cagcaaggcc cacaccgagt gtggcaccgg cagaattgga attatatccg gtgataccgg
    16921 cacctaacgc tgcaccaact gctgcgcctt cataaccacg gttcagccat ccctgagact
    16981 cccgcagatc catcttatcg gccttcagca cattcgcctg aatccagtag taggctttat
    17041 ccggagaagt caccacctga tacccttttg ctttcacagc atcagcaatt ttgccctgca
    17101 gcccactcat gtctttatca gacgtgtttt tgatctgcag aaataccgtg cgttcgctgg
    17161 cgggttcaag ccagatggtc tcactcatct gagtcttcac ctcaaggtta cgcttcttga
    17221 ttgctgtgct catcgcacca caccctgaaa gggccagagt ggaactgacc agtgtaacca
    17281 tcatcaattt ttttgttttc atcaaattac cttttttaca aactacagtt ttcttattgc
    17341 ctgtcataat agtcctgcca acgagtccca aaagtatttc ccgctgaatc aaagcctcca
    17401 tgcataggat atccggttga agggttatag tagatcgttg gggtctgttc aatgcccccg
    17461 aagtctaact tttcactccc atcatattgg tctttgagtt gattttgccc ccattttttt
    17521 atgcttatgc aaccactaac ttgccttaat tcgtatagtg taccaatgat aaaaaccatc
    17581 aggcttaata acggagagag taaggaagat actggcatcg ttatatacca aacgccttta
    17641 ctaaacttaa actcatccga cacatcccgt ttttttcgta tttgagagaa caaccagata
    17701 aacagtccct gccatagcac tatgaagaac agcataaaaa ctgtttcggc attcccccaa
    17761 tcagccagat aacaggcgat aaactgtatc actgttacca gagcaatatg ggctaaacat
    17821 ctcattatca tctcccaaac acagtattat tgcttatact tatcctgata ctcctttgcc
    17881 cttttcatca gttcctgtgg actgtcagcc ccgggcattc tttcctgagc cgacttttct
    17941 tcattatatt tattattctg agataatgcc tctgttttat ggtgattctg cagttcagaa
    18001 tactgtcctc ttacaatatt ttcctcgcca cgtatgctgt tctgagtatc acctatattc
    18061 cctttatatt ctgtgaccat attatcaacc tgatgtttta catcattacg gatattactg
    18121 tcctgcgttc tttgctcaat aatggcctga tgtccctgat ggtcagcaat aatatcctga
    18181 ctgcctccac ccgaaggtac gctctccatt cctttaccaa tatcgccacg ggattcccgc
    18241 caggcattat caacaccagg ctgtatctgt tcctgcacaa aagaccaggc cattgcccga
    18301 cggcgttctg caatctccgg cgaactggta tttgtcagaa tagcttcggc atcctgcggt
    18361 gcgtgtttca tcacatactg tgcaaactgt tgcgacaaat cttcgctcat ctgcccactc
    18421 atgctttcag tacgggatgc catttcagca tattcatggc tgcgggtcat attcgtcgtg
    18481 tactggtcgt aactctgttt cgctgaattc agggctgcag acagttgatc cacccgggaa
    18541 tcagcattat tgtcagtatg gctgccggat tcactgactt tgcgactggt aaagtaatcg
    18601 ctggcctctt taaagtcctt actggcttta gcgtcaatat catgacgagc atcatagctg
    18661 gcccgtgaac cactgctggc ctgatgcgca tcatcatcac tccagtcaat gccagcccga
    18721 ccaccacctc taacgcctaa tcctccacca acaccaagaa ttttaggttt aaccccccat
    18781 tcagcatgag catctccata catcccggca gaagctctcg tacttctgga tgccagttcc
    18841 tgagtcgcct gctcattact gatattatgc gccttcgcat agctttccac tgcactgcgc
    18901 atacggctgg ccatcatgga gtcctgtgca ctcatcgtac tgtcagcacc actggtgaca
    18961 gagtcactgc tcccccggtt agaaccaaac tgactgagcg tgttccaggc actggcaata
    19021 ctgctgctga aaccatgcag ggcactttct gctctgttcg acgcctcccg ggccatttcc
    19081 tgttgtgcca ctgcaatctg acgcgttgca ttgataccga ccggtaaacg ggacatcgct
    19141 ccgcttgcat ccatcaccat attaccgtca cgggtctgtg tggcggttgc gccactgccg
    19201 gtctggtaca tcatctgacc aaacgacgtg gtgctgttgg tgctccagct gaagccgttc
    19261 acgttttccg tctgcatgtt gccgtaggag taattaccgt caaccacact acctgccgca
    19321 ctggcagtcg ggctgatagc agaggaagcg aaatggctgt acacactgga aaatccggcc
    19381 ccaagccccc tgaccatcat ccaggataat ggcgggatca tcatggaaag atacccggca
    19441 gtggaagcca gatcggagta tttcagttgt atctgtgaaa tttcagagag caccaccggc
    19501 gcaccattct gtttcgcata aaatgtcatg gcactgttca gaatggcgta caagagtggc
    19561 catgactgca gccacatcag ggcaaacaca taacctttca ggacagacag cgtcagtttg
    19621 ttgaatacag ccgccagtac caggagcggg aaaataccga tggcgatccc cgtcaggatg
    19681 gtctgcgtca tgggcagtgt ccgcatcgcc acatgaccga tggagacatg tgccagacgt
    19741 tgtttctcca tcgatgacgt ggtggccaga ttcaccaggc tggccgaatc accattacgt
    19801 gcggcataac tggtgatccc ctccttcagc gcattgatgg tgacattctg gcggatgatc
    19861 tggctggctg atttacttga gccatagaag taactgtaac tgtcgccaat cagcgtactg
    19921 aacagcaggt taggatcagg gcgtccacca aatagctgct gggcataata atgccaggtt
    19981 tttccgccac tctgtgtgtc cagattcagc ttgtctttca gcgaaaccga cgcgtcctta
    20041 caggttacga aattattatt gctgtcataa acgccccgca gcgggctggg gcgggaaaaa
    20101 atcagcgtgt agggatcagc ggaggccatc agctcctcca gcgtgtattt gtggttcagg
    20161 taaatatccc ccataacaca gttctgcaca taatcctgga aaagattggc gatttccgga
    20221 ttgcgggaca aaaagtcggt gctttttgat accagttctg cgccaaacag catccccgtt
    20281 ttgctgtagg tgacactgtc cggttgcgtg aagatcatct cgtaactggc caccattgca
    20341 tgcccgatac gggtcgtcag tgaaagtggc atcgccagtc cgaccggcac attatccacc
    20401 cggtgaactt tgaccaggtc actgttatca ataatctgca ccgatgtgcg gacattaacc
    20461 agcaggctga taagcacaaa tacggccacc cagcccagca aatccatcac gttgtgccgc
    20521 tgtacccaca ttacggccac cgcgagaaca gacaatgtga gcgcaatttt ttcaatggaa
    20581 tcccacgtcc tggttcccat aaaggcggca atggcgttca ggttattccg caaccactca
    20641 ccaccggcaa tcacataaac ttcattcacc gcgtgctccc tccgaagtga tagttgtttt
    20701 ggtaacgact gagcatgcgg gcggaaagct gctgacgcat gtagctcatc tgacgatcga
    20761 caaccagcag cgcatcctgc tgtacctgca cctgtgactg aaacgccgca atctgccggg
    20821 tggcatcatt catgttgtcg ttaatatgtc cgataaccgc ttcgtcataa tttcccgtgg
    20881 ccaccatcgc ccgggcctgc tgtatcagct cctgaatgta ctgcagcagg atgtcgtaac
    20941 cgatatagtc cgtcagctgg taaatcatgc tgttggaaac tccgagcatc tgcgggtcaa
    21001 ccaggtattt gaagacgggg atggtggtac tggaaataaa gcctttttcc ttgtcatcca
    21061 gaggcgtgtc actgaccgcc ttgttctgaa tgctggccag aagtttagta atctgagact
    21121 tcagtgcatt atcccggctg atagtcaccg gtgtgtctgc caccactttc aggcatttat
    21181 cagaatcgtt gcagtgataa atttttgctg tgccgccttc catcatggcc cggataattg
    21241 agcggtcagt ggttctggct gacagcggtg tgatttcgcc gttgggacca aacaccagtg
    21301 agccggtcag cgtcatcaca aattctttca gctctttgtt gccgtcaaac attctgtttt
    21361 tggaaagcgc attccacatg atgttgatgt ttttggtcac ccgctcctta tccttgtcgc
    21421 tggctttatc cctgacactg tcagatttcc cgccaacggt acatccctgc cgggaagccg
    21481 cccagtcagc aaaaatattg ctctcaccgg caatgtcctg acagactttc tgctgggaca
    21541 cctgcgtccg ggggaaaagc ccgccgataa tcccctgtgc agcctggcag gaactgaggt
    21601 tcatactgtt aatgtcgctt gccattttct gcacgaagtc ttttgcggtt tttatttccg
    21661 gcaccgttgt ctgcagggca aggtcaaaaa agtaaccggc agcattgctc ataatctgct
    21721 taacaaaacg ctgcaactgc tcaccattaa taaaactgaa cgagccgaga taggcgtcga
    21781 tgccgccaca tccagcattg atatccggca gcgtcatgga aattagctga acatttttga
    21841 cctgagtacg ggcatacagg gagccaccgt aagcatagcc ggcggcctgc ccctgccaga
    21901 cgcccggctg tgtggtattg gaagcaaatc ccagcttatt aaagaactga ttcatgtcgc
    21961 tgttcacatc cgctgacgct gcaggcgtca ccgtcagtaa ggcggcacaa agaaccagaa
    22021 gaggtttaat gcgtggcatc attcttgccc tcccttaatg tgttctcccg gtgcatacga
    22081 catttcagca ggaatatctc gatatttccg tatattaatt cgtacattgg ggacagaaaa
    22141 aaatgtaaca agaaacccgg ctgcaagcgc atataacgca tacggaatgg catatatcag
    22201 gatattccac atataactcg aattatttaa ttccgggtcc gctttccacg aatcaataca
    22261 ggcatcaaaa ataatatcca taactgtcca taaaacaatt ccggcagtca ggaataccag
    22321 agacaaccca gcatacctgc cagtaattct ggaaatgtat ttataatctc gttctgtact
    22381 tctgattttg agctcaggat tacttttatt ttctctcatt ataagttccc tctgatattt
    22441 ccgatgattc attcagaccg gtcatgcggc tgtaatatat ttcagacacc gaaatccatc
    22501 ccacaattgc ccccacaaaa aacacaacag aaacagaaaa ggtaagactc agcgaaacga
    22561 caatttctac agggctggcg ggataaaaaa gcccctgacg gataaagggg atattcaggc
    22621 tgacgattat tcccgtcacg atgccggata ttccggcaat gagtaacacc acctgcttat
    22681 ttcgcacctt tttttcctcc gtacatctgc aaaacggtat ccacccgcgc cataaaaccg
    22741 gcggcatccg ttgccccctg taaaagcggt aatgcctcaa gcgtgttgac gttgaccagg
    22801 aaggtggtcg gtgtggccac cgggatattc gggaagaagg tctgcatcac ctccggtggc
    22861 accggtaatg cttcaggaaa ggccgtatcc ccctgaccat ccagggtgta ggaaaaaacg
    22921 gaaaagccgt actgctgtgc cagctgtttc agtaccgggt caaactggtg acagtaaggg
    22981 caatgcccct gcataaacag caccaccttc cagtcggcca ggttaacctg cctgccatta
    23041 ctgaggcgga accagcgggg agccggcttt gctgtcctgg ctgacgtgcc tgctgccggt
    23101 tgtgcaggct gcgtagccat accctgcgga ttccagagtc gctctatttc accccgggtg
    23161 gaggcctgca cagcagcggc gctcagcaac agggtgaaca gcagtgattt agtgagagac
    23221 atgtccgccc tcctgctgcc cttccatcat ggcctgttgc ctggcttcca gtgcctcacg
    23281 ctcttcccgg ctgacacggg caatccatgc atccagcacc tggtatgcgc cgaacagtac
    23341 gagaatggcc gcaatcagct ccgccagaaa aaaagccatc cagggtttgc tgtaaacaag
    23401 acgggcgacg atatgaaacc agacacccag ggaaaatacc cacattcccg taatatcaag
    23461 ccggggtaaa gagaatctgc gtttacttat catgttaaac tccggttatt atttacatga
    23521 gagtattcat gtctgtaata ttcgcgtttg tgtgatatta atcagaggta caaatggaaa
    23581 agcgatcttt taaagaaaaa ctggaaataa tcagaaacat aataagagag tcgttaccgg
    23641 gaaatgcggc aattattgcg ctgatatatg cagcatcaca ttcattaccg gtcaatgcct
    23701 tccctgacta tcttgtaata agtttattaa gtatcgcagc aggcatcgtt gtattatggt
    23761 tattttcaat tatctatatt tatttttgtg agctattcag aagccactgg attgcggtat
    23821 ggtttatcat atggtcttca gtaatcaacc tcattatttt gtatggtttt tatgaccgtt
    23881 ttatatgact tagcctgtca taagccccct tataaaaaac cacatcactc cggaccatac
    23941 cgatgacaac acaatacaga taaatattct tctgaacctg tcgaagctcg taacgtccgg
    24001 taatgcggcc gcaactattg aaacaaccag tgctgcgccc tgactcataa ttgcaccaca
    24061 cagaaaccag agactggcgg taagcacata taaacacgcg atattaataa caaggaaaac
    24121 cagtgagctc aggagaaaca ttttcctgtc attacgctca gaaaagaaat aaccggcaat
    24181 aatgacaaat attaacagaa caacacctgt aaacattttc aaataatctt cactcataaa
    24241 tcaaatccgc gattaaaaat tgggtttaaa atcttcagaa acgttcagga actgttttgc
    24301 caggtcgtcc tgcgaaataa agccgtatga taacgggcga atactgccct gtttcgggtc
    24361 aaccagcatc atggccggga aatatttcac gccgaggcgc tgcgcctgcc cctggtcagt
    24421 ccgggaatcc ggcaacagcg gattaatcac gccatccacg gaaacgggaa taacactcag
    24481 accatacgta tcccggaagc cattaatgac ctgcgccagt tgcccgtcga tggggtcctg
    24541 cccccggtaa aaaaacatga tgccgtagtg ttcagccagt ttcgcaatgg cctgtcgctg
    24601 ctgcgcctgg tctgctgcca gctggttccg taccgtgccg ttgtaatggc tgtactgcag
    24661 gttatagtcc agttcaggat gtgccagcat ggcctttctg gcgctcatgg tgaaaagccc
    24721 ggcctgctga gtccagtaat tttgcagccg gaaatatttc acaaaattat ccacgccggg
    24781 atacagaatg gcttcgtaca gcgcccgctt tgttgccgtc tgcagtgcgg ccagtttctg
    24841 cataatatcc ggttcctgac ggggggctgc agatacaggt ttattttctt tttccttcgg
    24901 atttattttc tcgttatacc actgccagcc tgcatctttt cctgacgccg gaaaaataaa
    24961 gcagcagagt aacagtggca gtaatgcttt attcatcttc attcctttca tcactttttc
    25021 tttcatcagg tttctgcagg tcagtcaggg cagaaaaaca aaaatacaga ataaacagga
    25081 gcacaccgga gtaaaggata acgaaagcag cggcaagcat cccgtctgcc gtggtttctt
    25141 ttaccgtatc ccacgaccag ataattaccg gagaaatgac aatcgctgta atcaaaagcc
    25201 ggataagaaa atcaataaaa cggttcgacg tgaatatgac tttcatcatt tcccccggtt
    25261 atttcccata ccttactgtc ctgcctgttt cagctggtca gcaatctgct ctttcacttt
    25321 ctgcgtcaga acgccactgt ccgggatttt ctggttattc atcaggtctt cgtagaagtt
    25381 agtgaaatcc agtctgttga actgaatttt ctgcagctca tcaaccgtaa tcccccgaca
    25441 gtcaggatgc ttcgcactac caaaactgat acgcaactga ccgttgcgcc cctgctgctg
    25501 gactatctgc gccagcttcg aatcaaactg gcagtagctg cgttttttct ccagacagac
    25561 acccagcact ttcttcgaac agaactcccc gacgctgacg gtcagtttgt ttgattttgc
    25621 tttagccagg gccttttcat cactgctgca tttggccagc ccgatatcct gtccccagcc
    25681 gctgtcctta cagcagttgc tgtatccggc tgcagccttc ttacaaaatt tcgcctgacc
    25741 ggtgaaagca cggacacccc ccccgttcag cgccgcaaca tccttacctg ctgcggccag
    25801 tgccgccagc tgagaaacgg cttcggcaaa atcattactc tgcccgctct gagccttatc
    25861 gcattcgcca tcgagacaga atacgtctcc cccacagacc atcactttcc cggatgtttt
    25921 tgactcacag gagtacgtgg catattcatg cagacaggca ccttcttcgg agtaaaaggc
    25981 acactggtgt gacaccaggg tacaggcagg attgtcggtg taggtctgac aggttccctt
    26041 gtctgctgac tgcgtgacat acgtgtcccg gtatgcccag cagctctggt acatgctgta
    26101 tgactgaccg tctttcacca gcgtcctgtt cccgccagcc tccgtacatt ctgtttttgt
    26161 cagtttcccc tgctccttgc tgaacgggca gctttcagac cagacaacat acggggtgta
    26221 cgaggctttt tttaccctga caaccagacg cagcttcatt gccgtatgac gtttgaattt
    26281 tgtattcccg acccggacgg ggtgagatga cgtaaaagcc acaccggcag tcagtgtttt
    26341 ctgtactggt gtaaatgagg catctgctga gtatttggca ttccagctca gtggggttcc
    26401 cagtactgta attgtcatag gtacactctt ctgcaagtaa gtacgactcc agctgtaatg
    26461 cgcatcgaca atttcaccgg taacgggaga ggtaatggag acaacatact gaccattcac
    26521 ttcacgtgca ggtagctgac tcatttcata ctccagcgtc cgggtttccc atgtggtact
    26581 cccctgcagc tccatacggg cagttcgtgt gcagtactgc tccacctgta aatcccgctc
    26641 acaggtgtaa ttcgtgtatt cactcctgct aatctcctgc gcactgcact gctgcccggt
    26701 gtttcccaca atgctgtcag cccggttcac cacatccctg ccggtctgga taaacggtgc
    26761 atccggtgaa agaatgtctt tcggcttgtt catgaatgac tctgtaatgg tttttccggt
    26821 ttcaccggtc gcccattccg tggtgccgtc atttttcagg ccgccatccc cgccggcagt
    26881 cacgccaccg taatattttg tctcgtccgg attcgcgtta tagccgggga tactctcctg
    26941 tggcttaaag ccctgaatac tgctgcttcc ctgccctttg atctggtgag caaaatcaga
    27001 accggcccgg taatcactgt tgctgtcagc ctgtgccatg ccggcaacca gagccagtat
    27061 cagaggtaaa atacgtttca tttcccggaa tctcctttcc ctgccagtaa atcatgagcc
    27121 acctgccggc agtcacccgt ggcggccact ttctccagcg cctggccgac ccgcaggttt
    27181 ccccggatga tgtcgtatcc ctgactgcag aacaccacca gtgccggtac agtgcgaatg
    27241 ccgtactgtg aaaacagcgt cgggtcgatc tgaacaccat cagtcgctcc gtctttcacc
    27301 agggacaaca cagcttcagc ggtggtcttc aggtcattgt tcaccatgcc ccgcagtgtg
    27361 gccggaatac cgtagtgccg ggtttcgccc agcattcgtt tcagcccctc ttcgggaatg
    27421 gaaaacgaca caaaatacag agcaccctgc cggggtttcc cgtcctgcga agcctgctgt
    27481 ttacgaacca gctcatccag gaaatggtta tctgaacgct gaaggggggt ttccagcacc
    27541 tgtttctccg cccaggcttt cagctgatgg tcaggttttt cacgcagttg tctgcttaaa
    27601 ttttcctgct gcttcaggaa ctggcggttt tcaggagtgt tcacgttttc tgacgccata
    27661 accgccccgt tcagcatcat cagcagtgct gccagagatt tcatactcag cttcatcatt
    27721 cacctcacag gaagacacag ttacgtttac gccacatcag gtagccgaag tttttctttg
    27781 tgttgggcgg atttttcccg gtttcccagc gggtcacgct gcgcccgaac gggtggcact
    27841 gcccgctgtc cggatacata ttcaccatct ggtaacgcca gcgttctttg ggcaggattg
    27901 gggacggata ttcattacag acggcgttat ttttcccgat ggtttccata atcatgccct
    27961 gacggtgcag cttgaacgcc atgcgttcac tgaccagcag ggaggactgc aacggactgg
    28021 actcattact cacccagcca ttgaacgggt acatacttcc ctgcgaaccg gcacaccaga
    28081 acagaacatc gagaggcata ttaaaggcgc tggcaatcgc atctgctgcg caggctccct
    28141 gtgctatcgg attggcaaag atgacagctt ccggattgag aatggtggtc aggctgctgt
    28201 ccgtccaggt ggggtcgatt tcagaaagat aagcgatatc caggtcacca ccttccagac
    28261 agcccagcga tgtgatgatg ttcagccagt acgtcagcgg gtatttgtac cagtgaacgt
    28321 gatagaacgc cccatttacc tgcttctcat cctttttcgc cgttccctgt gccgttttac
    28381 caaaagccgg caggctgaag cccaggttca ccatgcatcc cggtgaccgg gtgacgtccg
    28441 tcaacgccat cggctcccag taaccaatgg ccagcccgat acgcctgaac agcggcggcg
    28501 gtgccggaca aatctgaatg ggcatcgacg ggttcgccgt gtcggggacc ttaccctgac
    28561 tgactttgat actgcccagc gagagcggga aaatacagct ccagcagata tctgtgatcg
    28621 ggtttacaaa acgcccctca caggcagaat ccgcagacgc cgcagggaca tgaccggcga
    28681 aaaggaataa catcagcagc cacagccttc gcttcatttt ctgccctcct ctgccggaat
    28741 aaattccacc ttcaggaagc gatcgccggg aacggcgctc acccgtgccg ggacctgatc
    28801 aatgccgagg cgctggcaga gcacaccgtt ctggtcaaag taaacacggc tgtccagaga
    28861 cttctgcatc tccgggatac tgccctgcac gaggataatt ttgctctcca gtgtgggcgg
    28921 cgtctggcgt ttcatccagg ccacctgcgc cggatcatcg ccattgatga aatacagcgt
    28981 ctggttaaaa ggaacatact gcagcggatt catcacttca ccctgacggg caaacacccg
    29041 cccttcgtta tcccggatat ccgcagccag tctgacggac gggtcaaaca accggctgcc
    29101 gtatttctcc gtgcgtccga taccgggcac ggcagggggc cgcagactgt tgcggatcac
    29161 ccgctcctta aaggcatcca ttttccggcc catctcaccg gactgctcca gtgccgtcag
    29221 gcgctgcatg atcaccgtca gcatgtccgg ctcctttacc ggccacagat cgccccaggt
    29281 accaagatcg gcggcggcca cactctgccc ccatatcagc agggcaatca gcccccggca
    29341 tctcatggtt ccgccctcat tcgccgggca atatcctgct ggatttcccg ggtgatatcc
    29401 ggtgccccct gtaccacagc aggcgacacc agaatgaccg cgtggtgttt ctgctgccac
    29461 gcctgcaggc tggcttccag tgccgtgtta aaacgcgctg aaagggcttt tgactgcgct
    29521 tctgacagtt gtttctgact ggctctgtca aaaaacgcat ccacggtctg tttcatgttg
    29581 aaggcgacag tcaccggtgc gttcagtctg acaatgccgt aactgacggc tgcgttcagt
    29641 aacaccattg ccaggcatcc cggcaccgtc caccaccagt ggctgcgtcg ttctgccatg
    29701 acgtcggcgg gtttctgcgt tgaactcatg ccacactcct gtatttctca tgttcttcca
    29761 gccaggcttc cagcgaagcc atttccggcc ctgacttctt ccacgccagc tgccacactg
    29821 cctcatgaat actcagcccc tctttacgct tctgctgcac aaactcaaaa tccgggccgt
    29881 cagagctgta catggcgcgg cttaacgggt ccacaaacag acggtgccag gaggaatggt
    29941 tttccacctg cagcaggaag gaactgaacc actggtcttt ggccgcacca aacttaccaa
    30001 tcatgtcgcg ctgcagtggc aggaactggt ccggatacag ttggttgtat ttcgcgaact
    30061 ccttcgcact ctgtttgagg ataattttgt aggaggagtt accccatgcc gcgcgggcag
    30121 cactggaggc cttatcagag tcaaagtcga cgatgttctg tgtgatggtg atataggcac
    30181 cggtatgacg gcgggccgta cggtagcctt tctcaataaa ttcaccgacc ttgtgatttt
    30241 tgaagtccag cagacgccag ccttcatcaa taacgttcag tttcttgagg ttacgcggcg
    30301 tgcggtacat cctgttctcg atgtagataa tcagggagaa catcaccgca accagcagtg
    30361 acggacggtc ttccagtccg cccagctcca gcaccaccat ttttgcgtca tcccgcaggg
    30421 acggctcatc agagttaaaa tactggccgt aagtgccgtt ggcagtgtac tggtcaagca
    30481 gcacaatcat ttcgtccaga cggctgcgga tagtcggtga ctcggcatac tgctcgctgt
    30541 cactggcgtt tttcaggaaa tccaccacgt catcaatacg tgctctgttc tctttggcca
    30601 gccaggaggc tctgaccgcc tgcaacagca gaccttcatg cacttcatcc aggttaccgt
    30661 tggggctggc catcactgac aactggtcac ggacacgctc cgctgactgg tcaatatcgg
    30721 tgatgttcgc aaacgggtta aaacgcaggg tttcaccgtc cagatacacc ccgcccatgt
    30781 tctcacacag ggacttgtat ccatccccca tgtcgaacac cacggcaaag cctccggagt
    30841 ccagcacgct gcggataagt ggctgtatca gcccggtttt accggccccg gaggtgccac
    30901 agaccgccat gttgtagttg gtgttattca tcccccggaa gaaaatatcg ataaacgcca
    30961 gctggttacg gtaggtgggt gccagcagac ctgccggtgt cagggggtta tccgccacta
    31021 acggcatcag gttggcaaca ttaaagctct ctgcgcgctg aaccactccg gcctctttca
    31081 actgtttaaa cagccctttc ccggccataa agggcaggca ggtcaggaaa ttgcgcatgt
    31141 ggttaaagcg cggtgaaatc agctcaaaac cgtttttacg aaagctgttc aggatgtcct
    31201 gctccacttc cagcgccgtt tcattattgt ccttgcagaa tgctgtgatg ttgaggaagt
    31261 aggacaccac ggaggactga ccggagccca gccgctgacg cagttccccc cactccttcg
    31321 cctctttctc cacggacgga aaccatttgg cataggaggt cttcgacttt ttctccaggt
    31381 ccatgtactt caggttggct tcgctgtggg ttttcacctg gtcttccacc accagagtca
    31441 gcgtcaggat gaacggacag gagatggaca gttccgggtt cagcaggttg ctgtagttgt
    31501 cggccacgtt ccacaggaag gcgatttccg ggttacgggc cagatggaaa ttcaggatgc
    31561 gggccgtgct gttcctgccg ttctcacgca ggcccagcgt caggtaatca gcccggactt
    31621 tcagatcaaa actgtcctcc acacactgat aattcagatc agaatacggg tccagctgac
    31681 gtcttttcgg gtacagggag tccgggttat ggttaatcat ctccccgaca atatcgataa
    31741 aggcctgtgc atccaccgtc tgtgtggtga tactggcccc ctgtaacgac gcccggatga
    31801 ttttcaccag gttttccatt tccagaatgt cggcccggct ttttttcttc gaaggagaac
    31861 agtacgagat aaacacccgg taatggcgca gggtcagggg cagattcatc ccctccggca
    31921 gcggaaactg tgtcgctgcc gctttcatat aataggcccg ggtaatggcg ttaaaccgtt
    31981 cagcctgttc acctgaccag gagaactcac gcagcccgta ttcaatcctg tcaccaacca
    32041 gctgactgga catcagatgg atacacaacg gaataccgcg cggcagtttg gtgcgcagca
    32101 tgtgatcgag ggcctccaca atggactcat tcgccccatt gatggggatg gcttccagca
    32161 taaagcccat cgtggtgtca ttcatgaaca gaccggattc ctggttgtaa tcacggtacg
    32221 gcagcagacg gctgaactgc gggaagctca tttcgcccag aacttcattg gcctttgcgg
    32281 attcgtcagg cagtttcagt gctgtgacga gggagttaac cgcctgagtg acggcctcaa
    32341 gtgggttatt cacatgaatt ccttagttga aaaataaagc gagaaatgcg gtagccatga
    32401 agggaatacc tgtaattaac gcgccatttc ccggcgcata tacaaagatt tctgttttct
    32461 gaatatccac tcatgttcat ccggaattaa atcactatgt tctgcgactg acttatgcat
    32521 aatgttttct ctgtctttcc tgatacgcct gacattcaac gcacaacgtc acaccgggaa
    32581 atattttccg ccgggcttcc ggaataggat ttccgcatgc ttcacagaga taaacaggaa
    32641 taccatgagc atttattttc tggcgtatcc ggtttattcc tgtcatggtc agttgttctg
    32701 tcactgaata tgcttcatcg gcttcatcac tcacggaata ttcctcccga catgttctga
    32761 cacgctaccg cccatccctt ttcacgcaca gggcactcgt tttacaggca gattttgaat
    32821 acagaaagcc cgccggcatt gccggctgat atacaacttc agggccagtt aattaatacg
    32881 tggttttccc cacgcagacg gtttaataac aaaaaacacg ctggatggct gatgataaac
    32941 atcctggtta tcaatataag gcgctatcca taatgctgcc gtctgctcac ccgttcttag
    33001 cggacgagga ggtgttaccg gcgtaactga actgaccgga acaaccgttt tcacttcccg
    33061 ggcagcggta aacagcgggc gaggtgccag cagtttctgc tccggatgtg ctgtcaccgc
    33121 aggtctgctg ccggacggag tggtcgcggt gacggtctgc accggcattg tccggaagtt
    33181 cccttcagcc aggcgcggca gtgatgccgc aaccggcttt gcttctgagg actgctccag
    33241 ttttttggcc ttctcattgg cctgctccat cgtcatacag gtatcggatg tggtggcgtt
    33301 acactcaaat tccgtactgg ttccggcaca gccggaaagt aataaggtcc ccagcagagg
    33361 aataaagaaa gaaatctgtt tcattgtccc tcacctttct catcgcagtt cacaaaatcc
    33421 ttcttcaaat ccctgaccgt acaggttttt acaatgtcag gattttcaaa aaccgtactc
    33481 cgtgatgatt tataaacata tgccacaatg cgtttcccac ccgcatcagg ttctggtgca
    33541 ctgacttcac ataacggagg tgttattccg gactcataca gcaccggata atttattttt
    33601 ttgacgctgc catcagaaac cagcttattc atcgaagggt tccttatatt tctgaattct
    33661 tcatcacacc gacagcagtc atattaaaat tgtccggcaa tgactcagaa ccgaatttct
    33721 ccggtgcagg aagtacgatt cttgtggcgc agttactgag aacagcatgc ataaaatcat
    33781 ctgacacggt tttacccggc acagagaggg ctgtaataac attaatatta cgcatgttca
    33841 tttttatctc cgttgtttct gtttgttctg tcatcaggaa gataaacagg gcatttcctg
    33901 atttttcccg gcagggaagc gtcttccgta taaacttcct tcagatgttc atctgcggtt
    33961 accacccgga tacatttcat ttttccgggt cgcaccggat atttcccggt gatcgctgcc
    34021 agcgagacat acatcagtgc cagtgttgcc cataatgcgg tgaccagttg catcagtgac
    34081 cggtcagtct ccctgacata atctgcagca tcagccgcgc ggtaatggca gatatcctgt
    34141 atttctgatc tgcctgaaga atcagaggaa gaaaaccagc attcactgac cgggaactgt
    34201 ccggcaggtg ctgtttgtct tacgaagtta atctccttaa ccagtaattt ccccggcgtg
    34261 gtgttgtcct tccataaaac aaacagagcc atcagggcca ttgtggccag tggcagaatg
    34321 acggtgtact tcagacacca gaacagtcct cgcagaaccc gggcgacaac ataccgggcg
    34381 gcatggcccg cctgacggga cgacgtatta tttgccatca ttgccccctc cctgactgcc
    34441 ggacataaac tgctgcggat tcaggttgcc cagctgcttc agcatctggt cagtattaaa
    34501 gccgttaaga tgactttcag ctgatggcgg aacaggaacc ggactttccg gattgtcttc
    34561 ttccgctctg ctctgcgtgc gttccagcgc catctcttcc acggttttca gctggaagcc
    34621 gtcctggaac accacggtca cttcgttgcc cgcaccaatc ggtatcaccg ggtgatactg
    34681 ttcggcacgt ttgatgtagt agtcactgag cgtctgtgcg gctttcgatg cgccgccacc
    34741 gatacccatt ttaaggacat cgccggcccc gtaagcagca gtggcaccca gcccgacagc
    34801 cggctgggag gcgcgctcca ttccctgacc gataccgtca acaaatcccg cgccccatgc
    34861 ccagccgagg attttgccgt tacgcatcac cacttcgccc ttgataccgt ttttaccccg
    34921 gaagctgaca tgccccttaa tcggcatatc aatagttttg ccgtccttca ggcagctgat
    34981 attgcgggta cgtacaatgg cacgctcact ggacacatcc ccccaggctt ccagaccaac
    35041 aaaacatccc gttgcgtcat acgtcttgct gttcggcatt tccaccaggc cggtgatacg
    35101 cagctgcatc ggcaccgtgg attcattacc ggtgacagag gcattggcat ccgccccttc
    35161 aatcagcatg gctttcgcaa aacttcctga cggaatgtac ggcagtgatg gtccctgttt
    35221 tccctcatta cgggtaaaca ccttacgctg tatccggtta ggcaccggca cagactggta
    35281 cgtcacctgt ggtggtggcg tgacaccatt cccgggataa aacgccgtcg gcggtggaac
    35341 agcaacgctg ccctgcggcg ggaaggatac gggggtgttt cctggctgtg gttcgccttc
    35401 cgggccgggc ggtgaggcag gcatctgcgg tacaggctca ccggtcgccg tgacgggatt
    35461 agcacccagg gcttttacct gctctgccag ggcggcattg tcctgtccca acttttcaat
    35521 acgacgctgg tcatcaccgc gctgtttgtt cagaacatcc agctcacgac ggatttcctc
    35581 atactgcttc tgcatctgcg ctgccgtcac ctgcatctcg gtggtggcat gctgacgcac
    35641 cttgtcatca aaggtcgtat ccaccacacc ggtcatatcc ggcacaggct cctgttcagc
    35701 cacggtttca ccgttaccgg acatgtccac atcagacaga tacagtgccc caccaatcgc
    35761 ggaggctgta ccgacaacca caatccccag ccacaggtac tgcttgcgtt tcacaatggt
    35821 attgatactg gccatcagtt gccctccccg tcacggatga catacacatc catgcgcgca
    35881 cctgccagta actggctggc aggctggctg aacatcacgg cacgggttcc cggctgccag
    35941 aagtcacttt cccggatatt cagggcagac agcgttttat tttccacggc aaagcggacc
    36001 atcttcaggt gattccccgt ccatacggca tctgccactg aagacagccc cgccggggcc
    36061 tgcagggttt cctttgtcac tgggacctga taccagcctg cgggtaattt tccgccacgg
    36121 acggcctggc tgatggtcac aagcagggat tcgtaaggcg tggacgtttc ccacgcaccg
    36181 gcttcttctc cggttccgcg caggtcactg accagctgaa tggtacgccc cgcgccttca
    36241 cggggaacgg cctgaatgga aagattcaga ccacgttctg tttccagaat gaacgtaaag
    36301 ggctttttgt tgacggtggc aaccaccaca ccgccggagg cggtctgctc attattggtc
    36361 agggcaccat ccagactgtt cacggcgata atccggtcac cgggaacggt aaacagattc
    36421 gggctggtat tgctgatggc aatactggcc tgaccaccgt tcaccattgg caccacggtg
    36481 ggggccagcg taccgtttgc ggccatcacg ctgcaggaaa aaaacaggct gccgaatatt
    36541 attgccgtat tattttttct catcgtctgt ttccccgaaa ttatccagcc aggtcacccc
    36601 gttttcccgc ttcagaataa ggatgtaatg tttgatatca gtgaaaggtt ttgaatcacc
    36661 aatccaggtt ttaagcacac ctcgaatttc cacacggcca tactgaggcc agacacgaac
    36721 actggtctgg aaaaaggctg agttcacgtt atcgttttta atacgcttcg cttcttcagc
    36781 cagaataact ttcatctggt tctgtgcgcc cgggcggata tattgcagaa gcgcctgatg
    36841 tgaggcatcg acggtttctg atgaaacatt cagacggagg gcaataaatg acagcgccat
    36901 ctgctgtaaa taagaggcgt cggcactgtt ctgtgacacg gcaaaggggg cattaaatgc
    36961 cattggcgtc acggcagtgc gttgctcatt ctgaagacgg tagttattca ccccctgaat
    37021 gacgttaaca gagaggctga gaacaataag cactgacata aatataaagg cgatggccat
    37081 tacacgactg gtacttaaac gggcaccgtg ttccatataa caatcctggt atcagttcta
    37141 tttaatccac tgccggaaac acgaatcggg aacattatga aaaataccgc gcagcagggc
    37201 tgttggcata taccagtaaa tcaggtcacg taaccaggaa ctgccccgcc cttttttcag
    37261 ttttttaatc ccgaaataaa ccagaaccgc tgcaccaata ccgaacagat atttcgatgt
    37321 tgtgataccc cagccaatac agattgctgc ggggatcagt tcatccagtg gcaggccaaa
    37381 ccagcggctc tggttggtca gtgtttccgg gaaacgatat ttcttaagtt tattctcgtc
    37441 tcccgacatc gttttatttc ctgtcagagg ccaacgacgg ccatacccac agcaataaat
    37501 acagagatga tggcaaaacc ggccaggaac ttgacgtttt tggtcatcat gtacatgaca
    37561 gcaccgacca gaacttcagc cagaacaacc catttaacaa cactggagtc cttaccgaag
    37621 gtcgccttaa ccgtggtgtt accgcttgcc atcaggtcct gaccactgct gccggcggcc
    37681 atcgccagct gcgggaagaa catcatcaga acagcagccg ggatcactgc gcgagccagg
    37741 cgaagcatat tcagacgagt gaatttagaa aaaaacgact tctttttgac gggcgcagaa
    37801 gcaccctgaa cacttaaaac agcattcata agttacctca atttcggata aatgaataaa
    37861 actagagtgt attaaatgtt atatcttcct ctttatctgc ctccctgaat atctccgagt
    37921 tataaaaatc aggaaacttg atcaaatgat caataactct gtctgcagcc tcatctgttt
    37981 tacaccatcc actacgattt ttggctgcaa taagtagctg gttagtttca tcatccagac
    38041 gcattgtcag atacttaacc agcgacttac catagatatt ccctgccctg ttaaactttg
    38101 gatagcggtg aagatgatct ttaagtctta ttactgcctc aaacgatcga cttcttccgc
    38161 ttctgttact tgcctcaatt aatagacttt ccacatctac aggtaatttt agttttacca
    38221 tcttacctgt tgcagaacgt gtaccaaatc ttttcaataa cacctcccgc tgtttatctt
    38281 ctggttacca cttatgttta cagaataatc agaaaaggta aatagacgga aagtctatat
    38341 tttattaaca ccttattaac gcgtatttat gatacacata gccttctcgt ataaataata
    38401 aataaacttt ttattgtaga gtaagataat taactcatct ttattagaaa cagagaagta
    38461 gtcgcatatc agagatgcgt gtttctttga tgtggagatg gatatattta atattcttgc
    38521 tatagcatta tgactgattc cggtgctata taagtaaaag acatcattta catatgatga
    38581 tacagaagaa tcatcactca ttcttctgat tatatttctg atatcatcac tgacaatcgt
    38641 tttttgatta taactgtctt tatgacgaac atgagcagca tcataaaatt gccacagaat
    38701 aatatttctg tgtccattaa gaaatgactg gacagatatt gtccagaatc tgttctgaat
    38761 aaaaacatcc tctacaagat taagatgcgt atgctcattc ctgtatgctt ccatctctgt
    38821 gacagagatc aattcacaaa aatctctctg cgacagaagc catttttcag cactttgatc
    38881 ttgtgtaaga aatgactcat gaaacagcgt attgcaaaaa ataaattttc cagtttcgtc
    38941 cctgatacag gctggatttg gataatcctg aataactgcc gtcagatttt ccagcagatc
    39001 tatttgacga gcatgttttt gttgaatacg atccatcgga tacataggaa cctcctcaca
    39061 aaggattcta tggacagtcg atgcagggag gagagaactc cctgcatcgg cgattttcaa
    39121 attcaaattt taacgtggca ttaaccacgt aattaatata ctaattatct ttcgtactgt
    39181 caccttttta aatcataaaa actctgatat ttgaacgaag tcaaatttct tttattcatc
    39241 atcatttttt ggaaaaaatc gttccatctc agatgatacc ttctccctga tatcttcaac
    39301 catattggca tattcaaact ttggatttcc ggagacatga ggactgagag actcaatacc
    39361 caaaattttc gctactgatg attgtgtttt tacaacgcat tcaagaagca atttattaaa
    39421 ctcagtctga ttaaatgcag actctttacg ctccatctga gcctcatgta cacgaagccc
    39481 cagttcaaga agcattgaag ctgttgctga aaaactgaca tctttttccc ttgccccttc
    39541 ctgtcgacgc ttctcaataa tcgcatttat tttttcatag gcatcattgc tgatatacag
    39601 gttcacctta gccattagag attacctttt ttgaacataa aataaagtta atttcgaaac
    39661 actaaaattc ataatgcaaa cagggacgca ccgctagcag cgcccctagc ggtatcctat
    39721 aaaaaaacac accgcgccgc tagcagcacc cctaatataa aataatgttt tttataaaaa
    39781 tagtcagtac cacccctac
//
LOCUS       DACAPB010000017        39748 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.017, whole genome shotgun sequence.
ACCESSION   DACAPB010000017 DACAPB010000000
VERSION     DACAPB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.017"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(599..1795)
                     /gene="hemY"
                     /locus_tag="IT904_000823"
     CDS             complement(599..1795)
                     /gene="hemY"
                     /locus_tag="IT904_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAP3190650.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1798..2973)
                     /gene="hemX"
                     /locus_tag="IT904_000824"
     CDS             complement(1798..2973)
                     /gene="hemX"
                     /locus_tag="IT904_000824"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP3190651.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPQGDTPAAAPQGE"
     gene            complement(2995..3735)
                     /gene="hemD"
                     /locus_tag="IT904_000825"
     CDS             complement(2995..3735)
                     /gene="hemD"
                     /locus_tag="IT904_000825"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAP3190652.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3732..4673)
                     /gene="hemC"
                     /locus_tag="IT904_000826"
     CDS             complement(3732..4673)
                     /gene="hemC"
                     /locus_tag="IT904_000826"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAP3190653.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5060..7606
                     /gene="cyaA"
                     /locus_tag="IT904_000827"
     CDS             5060..7606
                     /gene="cyaA"
                     /locus_tag="IT904_000827"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAP3190654.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTPRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7646..7966)
                     /gene="cyaY"
                     /locus_tag="IT904_000828"
     CDS             complement(7646..7966)
                     /gene="cyaY"
                     /locus_tag="IT904_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAP3190655.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDYWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8014..9339)
                     /locus_tag="IT904_000829"
     CDS             complement(8014..9339)
                     /locus_tag="IT904_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAP3190656.1"
                     /translation="MNGQIITVALILLGHIFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLMDSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9336..10805)
                     /locus_tag="IT904_000830"
     CDS             complement(9336..10805)
                     /locus_tag="IT904_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAP3190657.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLKDWETVPPATDLTTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLPAPFGPWDTLARTRAKILINSEQLDADPNTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHVFES
                     DGPLILLDNHSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11171..11725)
                     /locus_tag="IT904_000831"
     CDS             complement(11171..11725)
                     /locus_tag="IT904_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HAP3190658.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYATGKMN"
     gene            12075..12278
                     /locus_tag="IT904_000832"
     CDS             12075..12278
                     /locus_tag="IT904_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP3190659.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12315..13139
                     /gene="dapF"
                     /locus_tag="IT904_000833"
     CDS             12315..13139
                     /gene="dapF"
                     /locus_tag="IT904_000833"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP3190660.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            13136..13843
                     /locus_tag="IT904_000834"
     CDS             13136..13843
                     /locus_tag="IT904_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAP3190661.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13840..14736
                     /gene="xerC"
                     /locus_tag="IT904_000835"
     CDS             13840..14736
                     /gene="xerC"
                     /locus_tag="IT904_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP3190662.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14736..15452
                     /gene="yigB"
                     /locus_tag="IT904_000836"
     CDS             14736..15452
                     /gene="yigB"
                     /locus_tag="IT904_000836"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAP3190663.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGVHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15536..17698
                     /gene="uvrD"
                     /locus_tag="IT904_000837"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15536..17698
                     /gene="uvrD"
                     /locus_tag="IT904_000837"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAP3190664.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17845..18609)
                     /locus_tag="IT904_000838"
     CDS             complement(17845..18609)
                     /locus_tag="IT904_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HAP3190665.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASSKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18979..19929
                     /gene="corA"
                     /locus_tag="IT904_000839"
     CDS             18979..19929
                     /gene="corA"
                     /locus_tag="IT904_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAP3190666.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19972..20352)
                     /locus_tag="IT904_000840"
     CDS             complement(19972..20352)
                     /locus_tag="IT904_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAP3190667.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(20366..20782)
                     /locus_tag="IT904_000841"
     CDS             complement(20366..20782)
                     /locus_tag="IT904_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190668.1"
                     /translation="MLTGDSYKDVKFMLRIFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(20841..21731)
                     /gene="rarD"
                     /locus_tag="IT904_000842"
     CDS             complement(20841..21731)
                     /gene="rarD"
                     /locus_tag="IT904_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAP3190669.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(21783..22250)
                     /locus_tag="IT904_000843"
     CDS             complement(21783..22250)
                     /locus_tag="IT904_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAP3190670.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRSGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            22415..23284
                     /gene="pldA"
                     /locus_tag="IT904_000844"
     CDS             22415..23284
                     /gene="pldA"
                     /locus_tag="IT904_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAP3190671.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            23417..25246
                     /gene="recQ"
                     /locus_tag="IT904_000845"
     CDS             23417..25246
                     /gene="recQ"
                     /locus_tag="IT904_000845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP3190672.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            25310..25930
                     /gene="rhtC"
                     /locus_tag="IT904_000846"
     CDS             25310..25930
                     /gene="rhtC"
                     /locus_tag="IT904_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAP3190673.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(25992..26612)
                     /gene="rhtB"
                     /locus_tag="IT904_000847"
     CDS             complement(25992..26612)
                     /gene="rhtB"
                     /locus_tag="IT904_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAP3190674.1"
                     /translation="MTLEWWFAYLLASIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            26723..27745
                     /gene="pldB"
                     /locus_tag="IT904_000848"
     CDS             26723..27745
                     /gene="pldB"
                     /locus_tag="IT904_000848"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAP3190675.1"
                     /translation="MFQQQKDWETRENAFAAFTLGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            27753..28553
                     /gene="yigL"
                     /locus_tag="IT904_000849"
     CDS             27753..28553
                     /gene="yigL"
                     /locus_tag="IT904_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAP3190676.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFQYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            28629..29528
                     /gene="bioP"
                     /locus_tag="IT904_000850"
     CDS             28629..29528
                     /gene="bioP"
                     /locus_tag="IT904_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAP3190677.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNTQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(29416..30369)
                     /gene="metR"
                     /locus_tag="IT904_000851"
     CDS             complement(29416..30369)
                     /gene="metR"
                     /locus_tag="IT904_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAP3190678.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            30487..32748
                     /gene="metE"
                     /locus_tag="IT904_000852"
     CDS             30487..32748
                     /gene="metE"
                     /locus_tag="IT904_000852"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP3190679.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(32788..33603)
                     /locus_tag="IT904_000853"
     CDS             complement(32788..33603)
                     /locus_tag="IT904_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP3190680.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            33865..34626
                     /gene="udp"
                     /locus_tag="IT904_000854"
     CDS             33865..34626
                     /gene="udp"
                     /locus_tag="IT904_000854"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAP3190681.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            34767..36194
                     /gene="rmuC"
                     /locus_tag="IT904_000855"
     CDS             34767..36194
                     /gene="rmuC"
                     /locus_tag="IT904_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAP3190682.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            36289..37044
                     /gene="ubiE"
                     /locus_tag="IT904_000856"
     CDS             36289..37044
                     /gene="ubiE"
                     /locus_tag="IT904_000856"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAP3190683.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            37058..37663
                     /gene="ubiJ"
                     /locus_tag="IT904_000857"
     CDS             37058..37663
                     /gene="ubiJ"
                     /locus_tag="IT904_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAP3190684.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            37660..39300
                     /gene="ubiB"
                     /locus_tag="IT904_000858"
     CDS             37660..39300
                     /gene="ubiB"
                     /locus_tag="IT904_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAP3190685.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            39379..39648
                     /gene="tatA"
                     /locus_tag="IT904_000859"
     CDS             39379..39648
                     /gene="tatA"
                     /locus_tag="IT904_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAP3190686.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            39652..>39748
                     /locus_tag="IT904_000860"
     CDS             39652..>39748
                     /locus_tag="IT904_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="mediates the export of protein precursors bearing
                     twin-arginine signal peptides; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase TatB"
                     /protein_id="HAP3190687.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTV"
BASE COUNT         9412 a        10071 c        10539 g         9726 t
ORIGIN      
        1 gattacataa ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa
       61 aatcaggaga gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt
      121 atatcgcgtt gattattgat gcttttttta gttttaacgg caattaatat atgtgttatt
      181 aattgaatga attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat
      241 tcactcagga agttattact caggaagtaa agaggattac agaattatct cataacaagt
      301 gttaagggat gttatttccc gattctctgt ggcataataa acgggtagat gctcattcca
      361 tctcttatgt tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg
      421 cttatcgtcc actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga
      481 gtgaagcacc cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca
      541 ggcgtttttt tatgcatcag aaaggtcccg gaggtgcttg cctccgggtg agaaggaact
      601 actgtggtgg gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt
      661 cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg
      721 gacgttgttt cagcgccgcg cggaaggcga gcgatgcttc ctgccattct ccgtgcttca
      781 tcagtgactg gcccagtgtg ctccacaaca gcgggcgatc gccgacgttt ttgatctgct
      841 ggcgtagcac tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca
      901 gtaggcgatc gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat
      961 gatcgtcaca ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg
     1021 ttttccggct ttggtttttc caccagttac gcaaaccttc gctaccgtta tcggcacgag
     1081 cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac
     1141 caacatgggc tttcgccatt gatgggataa tatccagcag cgaactccat gcacctgtgc
     1201 ggatatacgc ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca
     1261 gcttatccac gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac
     1321 gggtgatttc taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt
     1381 ggttggcgcg cgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca
     1441 ccaccggttg ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat
     1501 cgccttccgc cagtttcagc agcgcctgtt cggtctgctt acgtgcacgg cgacgcttac
     1561 gcccgacaaa ccacccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact
     1621 caatggcaaa cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg
     1681 tttcgatatt gtagttgtcg gtttggatca gcacataacc ctgatgaccg gcaatcatcg
     1741 ggccaaccac tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta
     1801 ttctccttgc ggcgcggctg ccggagtatc accttgcggt gcaggtttag cttccgttgt
     1861 ccctgctgcc ggttgtgcca gcaggttacg cacgcgagtc tgcatcagtt tttccagcat
     1921 cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc ttaactggtc
     1981 cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag cacgtaccca
     2041 ggtggagacg ttctccagcg cctggcgata agtctcttcc tggtgacgcg gtacagcttg
     2101 tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct gatttggcgc
     2161 taacagcggt acggcggtgt catcacgacg gcgaatcgta atgaagttgt ccataaagtt
     2221 ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa gctcttcacc
     2281 gtctgaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt tatctacctg
     2341 atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg atactgcaga
     2401 aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg ggtcattcat
     2461 atccgccagg ctggcgtctg cacttttcag caacgctgca gcggtcgtga cgtcctgatc
     2521 gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca gcagccaggt
     2581 tttagcatcg ctgccggaaa tggtggcgac cttttgttgg acttcatcca actgttttgc
     2641 cagcgtttct tgctgacgat tcgcctgctt aagttgtgca gcttgttgct taataatgcc
     2701 ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt ggttagccag
     2761 ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat acaaaccgac
     2821 gcccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcgg tattgttctt
     2881 actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc tggtctcttc
     2941 aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg agagttattg
     3001 taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt cttgccagcc
     3061 cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac agtgtagtaa
     3121 ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca tttcaccgct
     3181 ggtaacaacg accgtcgtca cctcgcggga ttgccagcgc atcgcttctt cggcaccatc
     3241 gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac cgcgcgccgt
     3301 cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg cacgtttgcc
     3361 cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc gatcctgcgg
     3421 gtagagaatc ttctgtccgc ttacggtatg tagtgccagt gcggtggtgc gtccaatggc
     3481 gaaataatta ggtagtcggg gccatttacg atcttgctga tgtagctgtg attgggcaaa
     3541 agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg ctgccagttg
     3601 atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa aatgccaggc
     3661 cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag acgggcgggt
     3721 gaccaggata ctcatgccgg ggcgtctccg ttatagactt cagcgaggat ctcgcgcgcg
     3781 ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatc ttgcggcgca
     3841 ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag cgcacgcagc
     3901 cagatttcgc catcaataag ctcggcgtag ctaccaattg gcacctgaca tccgccttcg
     3961 agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc
     4021 gcggcaagca gctcgcgagt gcgtgaatca tcaaggcggc attcaatacc caccgcacct
     4081 tgtcctaccg ccggaagaga aatctcgggt ggcaacgcgg cgcgaatacg tgactccaga
     4141 cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc gttatccagt
     4201 ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc cggacggcgt
     4261 tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc tgccggtaac
     4321 gcatccagac tgtcatagtt attggacaca aaggcatcgc gaggatcttc acgctcacaa
     4381 atagtgacca gtcccagacc ttgcgggaat tcaaccggca catctttcat tgaatgtacg
     4441 gcgatatcgg cgcgattttc gaggagcgcg acttccagct cttttacaaa taagcctttt
     4501 ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc
     4561 agttcaacga ccaggcccgg atggctcgcc atcaacttgt ctttgacata gtgtgcctgc
     4621 cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa catgcttgtt
     4681 accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt cagtgttgtg
     4741 gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat cctgacaggc
     4801 gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac ttgtatgtag
     4861 cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt agaccatttt
     4921 ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct ttgacatacg
     4981 aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc atgacgggta
     5041 gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga gactggatgc
     5101 cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat tccaacaggt
     5161 ctacagtcta ctgccgacat tgttgcacta tcaccatccg ctaatgccgg gttaccttga
     5221 tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac gccactacct
     5281 gaacgagctt gaactgtatc gtggaatgtc agtacaggac ccgccgaaag gtgagcttcc
     5341 aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt gttcctctga
     5401 cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc agttgctaca
     5461 acgtaaatgt agcctgctgg aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt
     5521 cctgattgat gaaaaccgtt tccgtcataa tgaaagtggc agtctggggg gcgaagattg
     5581 tggctccacc cagcatatac tgttgcttga cgaattttat cgtaccgccg tgcgtctcgc
     5641 cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt acgacgacta
     5701 tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg atctcggtgg
     5761 cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc tctacaagag
     5821 tatcgattcc ccatacaaag cggtactgaa aacactgctg ctggaagcct attcctggga
     5881 atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg acggcgagat
     5941 tgtatcgttt ggtctcgatc catactgcat gatgctggag cgtgttactg aatacctgac
     6001 ggcgattgaa gattttaccc gtctggattt agtacgtcgc tgcttctatt taaaagtgtg
     6061 cgaaaagctc agccgtgaac gcgcctgcgt aggctggcgt cgcgcagtgt tgagccagtt
     6121 agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc gcgctaactg
     6181 gaagattgat caggtgcgtg aggcgcacaa cgagttgctc gacgcgatga tgcagagcta
     6241 ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga
     6301 tatcggcgtg ctgacgcgta agctgtatgc cgcgtttgaa gcattaccag gtaaagtgac
     6361 gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct ttatttatgt
     6421 gccgccgggc cgggctaacc gttcaggttg gtatctgtat aaccgcgcgc caaatattga
     6481 gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaataaac tggtggcgtg
     6541 ggcatggttt aatggcctgc tgaccccgcg cacccgtttg tatattaaag gtaacggcat
     6601 tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg
     6661 cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagatccgcc atctggcgat
     6721 tatcgttaac ctggaatatg acccgacagc ggcgttccgc aatcaggtgg tgcattttga
     6781 tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg taggtagcgt
     6841 tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca acggcgagca
     6901 atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg ccgcaccgcc
     6961 agatagcgtg gaagtcttct gttatagcca gcatctgcgc ggcttaatcc gtactcgcgt
     7021 gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc aggaaaccgg
     7081 gcgtttcaag gcgctgcgcg tttctggtca aacctggggg ttgttcttcg aacgcctgaa
     7141 tgtatcggta cagaaactgg aaaacgccat cgagttttat ggcgcgattt cgcataacaa
     7201 actgcacggc ctgtcagtgc aggttgaaac caatcacgtc aaattaccgg cggtggtgga
     7261 cggctttgcc agcgaaggga tcatccagtt ctttttcgaa gaaacgcaag acgagaatgg
     7321 ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtatatcacc actgcgaagg
     7381 cagcaaagag gagctggtac gtgacgtcag tcgcttctac tcgtcatcgc atgaccgttt
     7441 tacctacggc tcaagcttca tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt
     7501 tgatggtcgt gaacaggtga ttccgttccg cacaaaatct atcggtaaca tgccgcctgc
     7561 caatcaggat cacgatacgc cgctattaca gcaatatttt tcgtgatgaa cgtgccggaa
     7621 agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc gcctgctgcg
     7681 tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa atccactcat
     7741 cgcctttcag atcaaaatgg tagccgcctt gtttggttgc cagccatacc tggtgcagcg
     7801 gttcctggcg gttgataatg attttgctgc cattttcaaa ggtaatggtc agtacgccgc
     7861 cgttgatttc gcagtcgata tcgctgtcgc catcccagta gtccaggcgt tcttcaatgg
     7921 tcagccagag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt gtatcctgtt
     7981 tttaagtgat ggcggcagta tagcggtatg gggtcagggc ttcaaagttt gcacctctgc
     8041 tgctgcgttc cggcatgatt catccgtcac cggaataatg atgtctctgt tttgaacttc
     8101 acgtacgttc tcctctctga cacgagtttt cgccgggcag ggggagggcg taatccttac
     8161 ctctgtatcg gtccatgtat aaacattgcg atcgtcataa gcgaagcgat cgtttttagt
     8221 tagcggaccg ttaactggaa taatttgcaa gctctttgac cgtaattggc ggataaagta
     8281 agcatgctgt ttgccgctat taaatgagcg atcaatgacc aggtctggcg tatccagcgg
     8341 gattatctca agctggtctt caccaaatgt cgataatttt gttacataca gccgatcttt
     8401 atattgcgca atattgccag taatcagatg aaattgttca ggttcaatac ctggtaggtc
     8461 tatccacttg ctccactgac ggtcgggcgt gagctgttta cgccagcgaa cgcgactttc
     8521 ccctacttca aaagagacgt catcatctac gggtatcgct ttgcctgata attgaccaaa
     8581 gggataacga tgcagaccct tattgtcgcg aaaaaccagc agtgaatgag gtatccagcg
     8641 aattatctca aatgagtctg cattcgccgt aaggtgaacg ccatttaaat aaatattctg
     8701 cccactgcgg ataagcagat cattggaata cgaatttctg tttacactca taagacgtgg
     8761 attaataccc caccatcttt tttctaatac agtaacatca ctgctgctcc cctgacgtcg
     8821 accggacaag tagagatttt tactatccat cagcgtggta gagttacctt cgatagcttt
     8881 tagtgtcgcc agatcaacac gtgacgcgcc gctattactt tcggtgcgct gtccgtcaaa
     8941 atagaggcta tatttatcga ccgcgaagcg gcccatagcc tgaaagctgg caatatcgac
     9001 agtgggggtt cccggtggat tgctaacgat tttgccacgc cagagaataa cgcggccgtc
     9061 tgtcacccag tggtattcct cattttttat gcttatttca tcggtgggta tatagccttc
     9121 caccaactcc cgctccccgg atatatttgt cacaaggcgc aaatcggctc tgttcatctt
     9181 cggaaattgg gcaatgattt tctcatcctc cccataacct ttaccgtaag acataacacc
     9241 gtgatcattg taatagtcga accctatggc ctgtacagca ggcgtcagaa atatgtgccc
     9301 caacaggatg agtgcaactg tgattatttg accattcatt ttgtcgcctc attgcgcggg
     9361 gcaggaataa attcgccgtc atcagtaatt tttccgtcca gatatcccgt tttgtaattt
     9421 acgccatagc catatcccga atgttgttcc ttccagatta agcgaacttg tttcggatca
     9481 gcggtgcggt atcgccagag ctgcgcgcca tcaaagctgt aaacgtagcg gttatcgcgg
     9541 gcataccatc tgcgcaaaaa gggatggtca ttaagatggg cttcgcgttc atccggagag
     9601 tggttatcca gtaagatcag cggtccgtca gattcaaaca cgtgtaaacc ggaatggtat
     9661 ggccaccctt cggcacgcgt aagtaaatag ccgtggcgtt tcccggcgtt gaaacgtttg
     9721 ttgataataa gattgggatc atcaagtttc acgatctcca gcttgcggtc accagactct
     9781 gtttcgatga tggtataaag cgagtcctga tactgggcga cagcatcact gatggggtga
     9841 aactgttccg gatcgagtcc gggtaattct tcctgtttac aagcaggccc tttacgccag
     9901 aacactttct tttccagcag agaaaaatcc agacagtgct ttgttaaatc ttcatcccac
     9961 gccagatttt ccttgtcgag gactttacgc tgtagcccgt ttttatcacg ccaggtcaag
    10021 agtgagccgg gcatccagcg tacgacggaa aaggtgttcg ggtcggcatc aagctgttcg
    10081 ctgttgatca ggattttggc ccgtgtacga gccagggtat cccaggggcc aaatggggca
    10141 ggaaggcagg gattgaatgc tgtagagaat ttaccgcgct gatcccatga tttttgtgcc
    10201 agtaccgtaa aggtgtcagg atcatcaagg cgctgaccat ttgcgtaaag ataatgcttg
    10261 tcacggagaa tcaaacctag ccagtctggt ttccattgcg ggttaaattc tacctgacgc
    10321 aacgttcggc ggtttagttg ctggttgcta tcggtgcgtt tgccttcgta atagaggctg
    10381 tacttatctg cggcaaaatc accccatgcc tgaaatgacg agatatcgac tggcggtgtg
    10441 cctggagggt tttgcacaat ctcgccagcg tagagaatag cgcgaccatc gctatagtaa
    10501 gagtaatggg gaagagttgc gtttgcgccg ctgtagacaa cgtctgtcgt gagatcagta
    10561 gccgggggca ccgtttccca atccttcagt tttgttgggt caattaacaa attcggtaac
    10621 agacgcagac gcttgtagtt aatctgcggt agcgaaagat tggtcttttc ataagggcgc
    10681 aggtacacca cgccttttcc ttgtttctgg taaatattat gttccggctc atcaagccga
    10741 catccccatg ccgatgataa gaacaatgaa caagacaaca aacaaaccac attgcgatag
    10801 tgcataaagc catcctggcg cgaggtgccg atcacgaaac taccagcaga acataattcc
    10861 ccgcacgtaa gcgtaacttt tgcggcaaat cgtcactttt cagaatccat aaaggtcatt
    10921 gctattttct gatagttgct gggtaaacgt gatgtttcca ttcgcgatat aaatacgagg
    10981 tactgcgcca tggtgtgact gatcacgtta aaatattccg gattgacgcc gggaagggtt
    11041 tcaacatgcg gctctgcccc cgttgcctac tttattgtct ttcgcgagtt gtacccaggt
    11101 cccaccatcg gtatctaaca ttccccacat tattacgctt tctgccgaac gtgattttta
    11161 tagaaatcta tcaattcatt tttcctgtgg cataaagttt atctttcaca tcctgccgaa
    11221 cgcgggtctt gtacccccca ttgtgaagat cttcatacgg agaaaaagaa tctttactcc
    11281 accccagcag cactttatcg ccaggcataa aatgcacatg cagaaaatct tctccccatt
    11341 ctcttttggg gagaggtaac gtcatattcc gtttctccag acgcattcca gcattatact
    11401 gtgtacgagt agtacttaat gtccagataa cctctgccgt atccccacta acatcaccac
    11461 aacaggttgt tgaaccgcca ccatttgcaa aagcattcgc gcccgccacg ccgttaacac
    11521 tgaatcccag tatcgggcgg tcaatttcac tgtggatttc cagaatcact ccacctcttg
    11581 gtcctcccca tactgcctgt cctatattcc acaacacgac cagtaccgga acgacaatta
    11641 acgtccgcca tacccagcgc ccccaccggg cataaccgcg cgtaagcttt tggtcaaatt
    11701 tttccagttg ggtaaatagc cccatcagat tttattccct cttatcacca ggttggcaaa
    11761 gttgtatgtc actggcgact aaggtcagac aacaaaattg catggtgcgc cactctttta
    11821 aaagctccat gccgacgata agaacaacga acaagccaac aaacaaacca cattgcaata
    11881 gtgcataaag ccatcctggc gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc
    11941 cccacgagta agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg
    12001 ctaaacgctt gcttttacgt cttctcctgc gatgatagaa agcagaaagc gatgaacttt
    12061 acaggcaatc cataatgaaa aacgtgttta aggcactcac tgtattactt actctcttca
    12121 gcctgacggg ctgcggtctg aaaggtccgc tctatttccc gcctgcagat aaaaacgcac
    12181 cgccgccgac caaaccggta gagacgcaaa cgcaatccac ggtgccggat aaaaacgatc
    12241 gcgccactgg cgatggtcca tcccaggtga attactaaaa gtcagtttct gtacccgcgt
    12301 gattggagta aatgatgcag ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg
    12361 tcgacgcggt aacgcagaat gtcttttttt caccggagct gattcgtcgc ctggctgatc
    12421 ggcacctggg ggtagggttt gaccaactgc tggtggttga gccgccgtat gatcctgaac
    12481 tggattttca ctatcgcatt ttcaatgctg atggcagtga agtggcgcag tgcggcaacg
    12541 gtgcgcgctg ctttgcccgt tttgtgcgtc tgaaaggact gaccaataag cgtgatatcc
    12601 gcgtcagcac cgccaacggg cggatggttc tgaccgtcac cgatgatgat ctggtccgcg
    12661 taaatatggg cgaacccaac ttcgaacctt ccgccgtgcc gtttcgcgct aacaaagcgg
    12721 aaaagaccta tattatgcgc gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg
    12781 gaaatccgca ttgcgtgatt caggtcgatg atgtcgatac cgcggcggta gaaacgcttg
    12841 gtcctgttct ggaaagccac gagcgttttc cggagcgcgc caatatcggt tttatgcaag
    12901 tggttaagcg cgagcatatt cgtttacgcg tttatgagcg tggggcagga gaaacccagg
    12961 cctgcggcag cggcgcgtgt gcggcggttg cagtagggat tcagcaaggt ttgctggccg
    13021 aagaagtacg cgtggaactc cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc
    13081 acccgttata tatgactggc ccggcggtac atgtctacga cggatttatt catctatgaa
    13141 gcaaccaggg gaagaactgc aggaaacact cacggagctt gatgaccggg cggttgtcga
    13201 ttatctgatt aaaaatcctg agttttttat ccgtaatgcg cgcgcagtag aagcgatacg
    13261 tgtgccgcat ccggtacgcg gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg
    13321 taatcatatt catgttctgg aagagaacat ggcgctgttg atggaacagg ctatcgccaa
    13381 cgaaggcctg ttttatcgcc tactctacct gcagcgcagt ctcaccgccg ccagcagtct
    13441 cgacgatatg ctgatgcgct ttcaccgctg ggcgcgcgat ctcggcctgg caggtgcgag
    13501 tctgcgcctg tttccggatc gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc
    13561 attaagccgt cagtctttcg aaccgctgcg tattcagcgt ttggggcagg aacagcacta
    13621 tcttgggccg cttaacggac cagagctgct ggtggtgcta ccggaagcga aagcggtggg
    13681 atcggtggcg atgtcgatgc tgggaagcga tgctgatttg ggtgtcgtgc tgtttaccag
    13741 tcgcgatgcc agtcactatc aacaagggca aggaacgcag ttacttcatg aaattgcgct
    13801 gatgttgccg gagcttctgg agcgttggat tgaacgcgta tgaccgattt acacaccgat
    13861 gtagaacgct acctacgtta tctgagcgtg gagcgccagc ttagcccgat aaccctgctt
    13921 aactaccagc gtcagcttga ggcgatcatc aattttgcca gcgaaaacgg cctgcaaagc
    13981 tggcagcaat gtgatgtgac gatggtgcgc aattttgctg tacgcagtcg ccgtaaaggg
    14041 ctgggagcag caagtctggc gttacggctt tctgcgctac gtagcttttt tgactggctg
    14101 gtcagccaga acgaactcaa agctaacccg gcgaaaggtg tttcggcacc gaaagcgccg
    14161 cgtcatctgc cgaaaaacat cgacgtcgac gatatgaatc ggctgctgga tattgatatc
    14221 aatgatcccc tcgctgtacg cgaccgtgca atgctggaag tgatgtacgg cgcgggtctg
    14281 cgtctttctg agctggtggg gctggatatt aaacacctcg acctggagtc tggtgaagtg
    14341 tgggttatgg ggaaaggcag caaagagcgc cgcctgccga ttggtcgcaa cgctgtggcg
    14401 tggattgagc actggcttga tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt
    14461 ctgtcgaaac tgggcaagcg tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg
    14521 ggcataaaac aagggctgaa taatcacgtt catccgcata aattacgtca ctcgttcgcc
    14581 acgcatatgc tggagtcgag cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc
    14641 aacctctcca ccacgcaaat ctatactcat cttgattttc aacaccttgc ctcggtgtac
    14701 gatgcggcgc atccacgcgc caaacggggg aaataatgcg tttttaccgg cctttggggc
    14761 gcatctcggc gctcaccttt gacctggatg atacccttta cgataaccgt ccggtgattt
    14821 tgcgcaccga gcgagaggcg cttacctttg tgcaaaatta tcatccggcg ctgcgcagct
    14881 tccagaatga agatctgcaa cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt
    14941 atcacgacgt gacgcgctgg cgttttcgtt cgattgaaca agcgatgctc gacgccgggc
    15001 tgagtgccga agaagccagt gcaggcgtac acgcagcaat gatcaacttt gccaaatggc
    15061 gcagccgaat cgacgtcccg cagcaaactc acgacacctt aaaacagctg gcgaagaaat
    15121 ggccgctggt ggcgatcacc aacggtaacg cccagccgga gctgtttggt ttgggggatt
    15181 attttgagtt tgtgctgcgc gctggcccgc acgggcgctc aaaaccgttc agcgatatgt
    15241 actttttggc tgcggaaaaa ctcaacgtgc cgatcggcga gatcttacat gttggggacg
    15301 atctcaccac tgacgtgggt ggggcaattc gcagcggaat gcaggcttgt tggatcagac
    15361 cggaaaatgg cgatctgatg caaacctggg acagccgttt actgccgcat ctggaaattt
    15421 cccggttggc atctctgacc tcgctgatat aatcagcaaa tctgtatata tacccagctt
    15481 tttggcggag ggcgttgcgc ttctccgccc aacctatttt tacgcggcgg tgccaatgga
    15541 cgtttcttac ctgctcgaca gccttaatga caaacagcgc gaagcggtgg ccgcgccacg
    15601 cagcaacctt ctggtgctgg cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg
    15661 tatcgcctgg ttgatgagcg tggaaaactg ctcgccatac tcgattatgg cggtgacgtt
    15721 taccaacaaa gcggcggcgg agatgcgtca tcgtatcggg caactgatgg gcacgagcca
    15781 gggcggtatg tgggtcggca ccttccacgg gctggcgcac cgtttgctgc gtgcgcacca
    15841 tatggacgcc aatctgccgc aggatttcca gatcctcgac agtgaagacc agctacgcct
    15901 gcttaagcgt ctgatcaaag ccatgaacct cgacgagaag cagtggccgc cgcggcaggc
    15961 aatgtggtac atcaacagcc agaaagatga aggcctgcgt ccgcatcata ttcaaagcta
    16021 cggtaatccg gtggagcaga cctggcagaa ggtgtatcag gcgtatcagg aagcgtgtga
    16081 ccgcgcgggc ctggtggact tcgccgagct gctgctgcgc gctcacgagt tgtggcttaa
    16141 caagccgcat atcctgcaac actaccgcga acgttttacc aatatcctgg tggacgaatt
    16201 ccaggatacc aacaacattc agtacgcgtg gatccgcctg ctggcgggcg acaccggcaa
    16261 agtgatgatc gtcggtgatg acgaccagtc aatctacggc tggcgcgggg cgcaggtgga
    16321 gaatattcag cgtttcctta atgatttccc cggtgccgaa actattcgtc tggagcaaaa
    16381 ctaccgctct accagcaata ttctgagcgc cgctaacgcc ctgattgaaa acaataacgg
    16441 gcgtctgggt aaaaaactgt ggaccgatgg cgcggacggt gagcctattt ccctctattg
    16501 cgcttttaac gaactcgatg aagcgcgttt tgtggttaac cgcatcaaaa cctggcagga
    16561 caacggcggg gcgcttgccg agtgcgccat tctctaccgc agtaacgccc agtcgcgtgt
    16621 gctggaagaa gcactgttgc aggcgagtat gccgtaccgt atttacggcg ggatgcgctt
    16681 cttcgaacgc caggaaatca aagatgcgct ctcgtatctg cgcctgattg ccaaccgtaa
    16741 cgacgacgcg gcctttgagc gtgtggtgaa tacgccaacg cggggtattg gtgaccggac
    16801 gctggacgtg gtacgtcaga catcgcgcga tcgccagtta acactctggc aggcatgtcg
    16861 tgaactgttg caggaaaaag ccctcgccgg acgtgctgcc agcgccttac agcggtttat
    16921 ggaactgatc gacgccttag cgcaggaaac tgccgatatg ccgctgcatg tacagactga
    16981 ccgggtaatt aaagactccg gcctgcgtac catgtacgag caggagaagg gcgaaaaagg
    17041 tcagacgcgt atcgaaaact tagaggaact ggtgacggca acgcgccagt tcagctacaa
    17101 cgaagaagac gaagatttaa tgccgctgca ggcgttccta tcccatgcgg cgctggaagc
    17161 aggcgaaggg caggcggata cctggcagga tgcggtacaa ctgatgacca tgcactcggc
    17221 gaaaggtctg gagttcccgc aggtgtttat cgtcggtatg gaagagggca tgttcccaag
    17281 ccagatgtcg ctggatgaag gcgggcgtct ggaagaagaa cgccgtctgg cctacgttgg
    17341 cgtaacccgc gcgatgcaga aactgacgct gacctacgcg gaaactcgcc gtctgtatgg
    17401 caaagaggtt taccatcgcc cgtcgcgctt tattggcgag ctgccggaag agtgtgtgga
    17461 agaggtgcgc ctgcgcgcca cggtaagccg cccggtcagc catcagcgga tgggtacgcc
    17521 gatggtcgag aacgacagcg gctacaagct cggccagcgt gtacgccacg ctaagtttgg
    17581 tgaaggcacc atcgtcaata tggaaggcag cggtgaacat agccgtttgc aggtggcatt
    17641 ccagggccag ggaatcaaat ggctggtggc agcttacgcc cggctggagt cggtgtaacg
    17701 ttgccggatg cggtgctgcg caccttattt ggcctaaaaa atcattcaga ttcaataaat
    17761 tgcaacgtca tgtaggccgg atagggcgtt tacgccgcat ccggcatctg cgccatcttc
    17821 agtatctgac acaaaactat cgttttaacc tttccgctcg acggaaatca tggtgacaaa
    17881 aggatagcgt tgccacggga ttgccccgcc tttcatatac atatgtgaaa tcgtgccatc
    17941 aagataaagc agctgctcaa cgttcagttt cgctttggca taacaggcaa aatcataaaa
    18001 atttgttgcc tgctggctca acaaaaacac ggcgttccca tgtttattaa tcccaacacc
    18061 gttacgaatt ttgcttgagg cgacgttggg atgaatacgc ggattaatta caccgttttc
    18121 catcaacatt ggccctgact gcaccgcaaa ctgaatctct ttactggttt tgaaggcatc
    18181 cagacgaacg atgccgactt tatctcccgc gacataaaac acgccgccag gacggataaa
    18241 gaaattccct tcacctgaag cgagatttaa cgccaccttc tgctgaccgt tttcgatgta
    18301 caaaccgagc ggcgcatagc tttcatcata gatgccgccg ttcatcgcca tctgcacctg
    18361 accctgacta ttcatatccg ccagcagagc atgtaacgtt ccccacgctt cgccattggc
    18421 tttttgccag tacattttca cccgctctgt ttgaggatta acggtatacg cctgtacggt
    18481 cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca gcaaacaagg gaagtaaggt
    18541 gagggcgaga aaaatccgtt tgagattcaa ggtgatcatt cctttaccaa tgagtagccg
    18601 atgcgccatt ataggtcctg gatgtgggat ttttttatcc tgttagcgac cttgacgagt
    18661 accaaaaagc gcgaagttca actattgttc tgtggtgttc tgttgcgtgt tgacggcaaa
    18721 attttgctgg cgtaacatgc gcgcacgatc actctaagag gacattcgcc ttggacacac
    18781 ccagtagata ctggctcact atcctgtcat ccaggatcaa ctcctaaggc tatccctttt
    18841 tgctgatagc cttagcggtt gtcagcgacc tcaatttttc ccgtcgcgct gagtcaggct
    18901 gtttaatggt ctgaaaccca atttgtttct gtgtgcccac cgaactgtcc gatattttaa
    18961 gcattgggag tcccggtcat gctgagcgca tttcaactgg aaaataaccg actgactcgg
    19021 ctggaagtcg aagagtcaca accccttgta aatgcagtat ggattgatct tgtcgaaccg
    19081 gacgacgacg agcgactgcg cgtacaatct gaacttggtc agagcctggc aacccgcccg
    19141 gaactggaag acatcgaagc atcggcacgt ttctttgaag acgacgacgg cctgcatatt
    19201 cactccttct tcttctttga agatgcggaa gatcacgccg gtaactccac tgtggcattt
    19261 accatccgtg atggtcgtct gtttactctg cgtgagcgtg aactgcccgc ttttcgtctg
    19321 tatcgtatgc gtgcccgtag ccagtcgatg gtggacggta acgcctacga gctgctgctg
    19381 gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa ttgaaaatat ctatagcgac
    19441 ctggagcagt tgagccgggt gattatggaa gggcatcagg gcgatgagta cgacgaggcg
    19501 ctctccactc tggcggaact ggaagatatc ggctggaaag tgcgcctgtg tctgatggat
    19561 acccagcgtg cgctcaactt cctggtgcgt aaagcgcgtt taccgggtgg gcaactggag
    19621 caggcgcgtg aaatcctgcg agatatcgaa tccctgctgc cgcataacga atccctgttc
    19681 cagaaggtga acttcctgat gcaggcggca atgggtttta tcaacatcga gcagaaccgc
    19741 atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc cgccaacgct ggttgcctcc
    19801 agctacggga tgaactttga gtttatgcca gaactgaagt ggagtttcgg ctacccaggc
    19861 gcgattatct ttatgatcct cgcgggcctg gcaccgtatc tgtactttaa gcggaagaac
    19921 tggttgtaaa aaacgagagc ggtggcttag tctggctaag ccacctgtta ttcaaaggct
    19981 ccaggtattt aaccctttta cctctttctc atagaaccat ttgttcgtgt taacagcaac
    20041 ataggctgct acggcaattc ccagaatgtt aacgccaatt agtgcaccaa caaatagaga
    20101 tagaatacca agtaatagaa ctataattgc ttttttccat aaccccagga caaataaata
    20161 tatccaggaa cagaagaaag caatgaaatt catttgaata gttaagcgtt gtcttacttt
    20221 taatgcttta aatgctgctt tatattctgg tgttgccccc cagaatccag gaaaaccatg
    20281 ttgatcataa aaattaaatc ggtatttcca tttttcactt aatgaaccat cgttcatata
    20341 ttccttactc ataaatactc cataactatt gttttgatga atcagtaggt gcaagcatta
    20401 gcatactgaa agtggaaaaa taacaaatca aaaaaatcat cgaaccattg cctgaacagg
    20461 caaaatcttc ggctatcatt gtgatgatag agatgatata tactgctaat gtaccaaaaa
    20521 cataagtttt tatatagatg aaaccactat cacggagtcg ctggcaattc atgttgatga
    20581 cgagataatg gagtacgatg gtagagacta taacaagaaa gcctgcttct ccatcgttaa
    20641 aaaagataat aagaaaggca aaaatgaaat ttattaaaat aaatgaaaat atataacgac
    20701 gtctggaaat cttaccgtta gatgttggga taaatatacg taacataaat tttacatcct
    20761 tgtatgagtc tccggtcagc atggcaatat gcccactctc atgcaagagt cggcatattt
    20821 tttcagaata tatttatttt ttatttggac gttctacgct gcgtataaat cgcatccatc
    20881 acaaaaattg ccagcgccac ccaaataaag gcgaaagtca ccatcttatc ggcacccggt
    20941 ttttcaccat aaaacgtcac agccagcagg aacatcagcg tcgggccaat gtactggaaa
    21001 aagcctaacg ttgagagacg caagcgcgtg gcagcggcgg taaaacacaa cagcggtacg
    21061 gtagtgacaa taccggcggc gatcagcagt aaattcagcg acatcgggtt ttgccccata
    21121 tggctggttg agctgtcggc aatagcaaac aggtaaattg ccgccacggg cagcagccac
    21181 atggtttcga ttaacatgcc ggtttgcgct tcaacggcaa tcttcttgcg taccagaccg
    21241 tagaaggcaa aactaaatgc cagtcccagc gcgataatag gtagcgaacc aaaagtccac
    21301 agctggacta acacgccaca tatcgccaga atcaccgcca gccattgcat ccggcggaat
    21361 cgctcgccga ggaaaatcat ccccagcaca atgttcacca gcgggttaat aaagtaacca
    21421 aggctcgctt ccagcatatg gtgattgttc accgcccaga taaacagtag ccagttgcca
    21481 ccaatcagca cggcagagac tgccagcata aaaattttct gtggcgtctg aatcagcgtt
    21541 tttaaatagg accactggcg gcaaatgctc atcagcacca ccataaagaa aaacgaccag
    21601 atcacgcgat gcgtcaggat ttcatcggcg ggcacgtagt aaatcaactt gaagtacgct
    21661 ggcgctatac cccaaataaa ataagcggca agagcgagta atacgccctg ccgcgtttgt
    21721 tttgcatcca tcgggaatac tcatttttaa ttggtaacag cagtttacct gcttttatgt
    21781 cttcaaccta ccatataggt ggcggtggca ctggcaatat aaagctgttc ttcattgtgt
    21841 aattcaacgc gggcgacggc gactttattg cctgaacgca acaggctact agtagcagta
    21901 aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc
    21961 gatagccgct ggcgtagttc atcttcactg atggtttcgt ggcgggttaa ggtacttccc
    22021 acgcacacca gaccggcggc gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg
    22081 ctttgcgccc agttgcccac catcattggc tgatttttaa aggccagctg tgcgaactct
    22141 ttttcgtaac gctccagttc catccccaat gcgcggttaa atggcatgtg ataaacaaac
    22201 atctcaccca ctaatttcag ggcttgttca gcggtcagta cggcagacat atcatcctta
    22261 cacttcattg gttaatgaaa tgttgatttt atgcttcttt gttgttggtt tctactttag
    22321 gaagggataa ctaacggcta tggagttaag tatgtaaaat agcccgcaga aaaatattca
    22381 ccttatcaat aattcgttac ggagaacacg accgatgcgg actctgcagg gctggttgtt
    22441 gccggtgttt atgttgccta tggcagtata tgcacaagag gcaacggtga aagaggtgca
    22501 tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg ctgcaggagc atgacaatcc
    22561 gttcacgctc tatccttatg acaccaacta cctcatttac acccaaacca gcgatctgaa
    22621 taaagaagcg attgccagtt acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt
    22681 tcagttgagc ctggcatttc cgctgtggcg tgggatttta ggcccgaact cggtgttggg
    22741 tgcgtcttat acgcaaaaat cctggtggca actgtccaat agcgaagagt cttcaccgtt
    22801 tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt gccaccgatt accgttttgc
    22861 aggttggacg ctgcgcgatg tggagatggg gtataaccac gactctaacg ggcgttccga
    22921 cccgacctcc cgcagctgga accgccttta tactcgcctg atggcagaaa acggtaactg
    22981 gctggtagaa gtgaagccgt ggtatgtggt gggtaatact gacgataacc cggatatcac
    23041 caaatatatg ggttactacc agcttaaaat cggctatcac ctcggtgatg cggtgctcag
    23101 tgcgaaagga cagtacaact ggaacactgg ctacggcggc gcggagttag gcttaagtta
    23161 cccgatcacc aaacatgtgc gcctttatac tcaggtttac agcggctatg gcgaatcgct
    23221 catcgactat aacttcaacc agacccgtgt cggtgtgggg gttatgctaa acgatttgtt
    23281 ttgatgaacg gttgagtggt tggcaaatct ggaacccagc atccaggatt accctctcag
    23341 agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca
    23401 ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat ctggagtccg gagctaaaca
    23461 ggttttacaa gaaacctttg gctaccaaca gtttcgcccc ggccaggaag aaattatcga
    23521 cactgtgctt tccggtcgcg attgcctggt cgtcatgccc accggtggcg gaaaatccct
    23581 ttgctatcaa attcctgcct tattgctaaa cggccttacc gtggttgttt caccgctgat
    23641 ttcgttgatg aaagatcagg tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct
    23701 taactcgacg caaacccgcg aacagcaact tgaagtgatg acaggctgcc gcaccgggca
    23761 aattcgctta ctgtatatcg ccccggaacg cctgatgctg gataactttc ttgagcatct
    23821 ggcgcactgg aatccggtgt tattagccgt cgatgaagcg cactgtatct cccaatgggg
    23881 ccacgatttc cgcccggaat atgccgcgct cggtcagttg cgccagcggt tcccgacgct
    23941 gccgtttatg gcgctgaccg ccacagccga cgacaccacg cgccaggata tcgtgcgcct
    24001 gctggggctg aacgatccgc tgattcaaat cagcagtttt gaccgtccga atattcgcta
    24061 catgctgatg gagaagttca aaccgctcga tcagttgatg cgctacgtgc aggaacagcg
    24121 cggtaagtca ggcattatct actgcaacag ccgcgcgaaa gtagaagaca ccgctgcgcg
    24181 cctgcaaagc aagggaatta gcgcggcggc ctatcatgcc gggctggaaa ataatgttcg
    24241 cgccgatgtg caggaaaaat tccagcgcga tgacctgcaa attgtggtgg cgacggtggc
    24301 gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg gtccactttg atattccgcg
    24361 caatatcgaa tcctattatc aggaaaccgg acgcgccggg cgtgatggcc tgcccgcgga
    24421 agcgatgctg ttttacgatc cggctgatat ggcgtggctg cgccgttgtc tggaagagaa
    24481 gccgcagggg cagttgcagg atatcgagcg ccacaaactc aatgcgatgg gcgcgtttgc
    24541 cgaagcgcaa acttgccgtc gtctggtatt gctgaactat tttggcgaag ggcgtcagga
    24601 gccgtgcggg aactgcgata tctgcctcga tccgccgaaa cagtacgacg gttcaaccga
    24661 tgctcagatt gccctttcca ccattggtcg tgtgaatcag cggtttggga tgggttatgt
    24721 ggtggaagtg attcgtggtg ctaataacca gcgtatccgc gactatggtc atgacaaact
    24781 gaaagtctat ggcatgggcc gtgataaaag ccatgaacat tgggtgagcg tgatccgcca
    24841 gctgattcac ctcggcctgg tgacgcaaaa tattgcccag cattctgccc tacaactgac
    24901 agaggccgcg cgcccggtgc tgcgcggcga atcctctttg caacttgccg tgccgcgtat
    24961 cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc ggcaactatg atcgcaaact
    25021 gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat gaaagcaatg tcccgccgta
    25081 cgtggtgttt aacgacgcaa ccttgattga gatggctgaa cagatgccga tcaccgccag
    25141 cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg gaacgctttg gtaaaccgtt
    25201 tatggcgctt atccgcgcgc atgttgacgg cgacgacgaa gagtagtcag cggcataaaa
    25261 aagtgccagg atgaagactc cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt
    25321 atttctcacc gtcgccatgg tgcatattgt ggcgcttatg agccccggtc ccgatttctt
    25381 ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctggg
    25441 cattacctgc ggtgtaatgg tttgggctgg gattgcgttg cttggcctgc atttgattat
    25501 cgaaaaaatg gcctggctgc atacgctgat tatggttggc ggtggcctgt atctctgctg
    25561 gatgggttac cagatgctac gcggtgcact gaaaaaagag gctgtatctg cgcctgcacc
    25621 acagattgag ctggcgaaaa gcgggcgcag ttttctgaaa ggtttactga ccaatctcgc
    25681 taatccgaaa gcgattatct actttggctc ggtgttctca ttgtttgtcg gtgataacgt
    25741 tggcactacc gcgcgctggg gcatttttgc gctgatcatt gtcgaaacac tggcgtggtt
    25801 taccgtcgtt gccagcctgt ttgccctgcc gcaaatgcgc cgtggttatc aacgtctggc
    25861 gaagtggatt gatggttttg ccggggcgtt atttgccgga tttggcattc atttgattat
    25921 ttcgcggtga tgccagacgc gtcttcagag taagtcggat aaggcgttta cgccgcatcc
    25981 gacattattt ttcacgcatg cctcgccgat gctaacagcg ctcccaccag cataaacaac
    26041 gagccgaaaa tcttattcag cgccttcatc tgctttggtc ctttaatcca tagagcaatc
    26101 cgttgagcaa gggtggcgta accgatcatc acaataatat cgactacaat agtggtgacg
    26161 ccgagcacga tatactgcat cagttgcggc tgctgtggca tgatgaattg cggaaatagc
    26221 gccgccagaa acacaatact tttgggattg gtgagattca caaaaactgc gcgctggaac
    26281 aaatgtcgac gcgattgagt agaggccagc gatttaaggt caattgcacc agcggcgcgc
    26341 cactgctgga ttcccagcca aatcaagtaa gccgcgcctg cccacttcaa cacttcaaac
    26401 gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc caaccagcac aatatgaatc
    26461 gccagtccgg tctgaagccc agcaatagac gccaccgcgc cgcgataacc gtggttgagc
    26521 gaggtggtca tagtgttgat tgcaccagag cctggcgaca ggcttaaaat gatcgatgcc
    26581 agcaggtagg caaaccacca ttctaaggtc atgatgaact cccggtgtgt ctatttttgt
    26641 gccacaatac gctactgtcg cagcgttgtg tcaggcacgc taaaaaaaac gattttacgt
    26701 ggtttaagag gcagattacc cgatgtttca gcagcaaaaa gactgggaaa caagagaaaa
    26761 cgcgtttgct gcttttaccc tgggaccgct gactgatttc tggcgtcagc gtgatgaagc
    26821 agagtttact ggtgtggatg acattccggt gcgctttgtc cgttttcgcg cacagcacca
    26881 tgaccgggtg gtagtcatct gcccggggcg tattgagagc tacgtaaaat atgcggaact
    26941 ggcctacgac ctgttccatt tggggtttga tgtcttaatc atcgaccatc gcgggcaggg
    27001 acgttccggt cgcctgttag ccgatccgca tctcgggcat gttaatcgct ttaatgatta
    27061 tgttgatgat ctggcggcat tctggcagca ggaggttcag cccggtccgt ggcgtaaacg
    27121 ctatatactg gcacattcga tgggcggtgc gatctccaca ttatttctgc aacgccatcc
    27181 aggtgtatgt gacgccattg cgctaactgc gccaatgttt gggatcgtga ttcgtatgcc
    27241 gtcatttatg gcacggcaga tcctcaactg ggccgaagcg catccacgtt tccgtgatgg
    27301 ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt gctatcaacg tactgaccca
    27361 cagcagacag cgatatcgac gtaacttacg cttctatgct gatgacccaa cgattcgcgt
    27421 cggtgggccg acctaccatt gggtacgcga aagtattctg gctggcgaac aggtgttagc
    27481 cggtgcgggt gatgacgcca cgccaacgct tctcttgcag gctgaagagg aacgcgtggt
    27541 ggataaccgc atgcatgacc gtttttgtga actccgcacc gccgcgggcc atcctgtcga
    27601 aggaggacgg ccgttggtaa ttaaaggtgc ttaccatgag atcctttttg aaaaggacgc
    27661 aatgcgctca gtcgcgctcc acgccatcgt tgattttttc aacaggcata actcacccag
    27721 cggaaaccgc tctacagagg tttaaatttc ttatgtacca ggttgttgcg tctgatttag
    27781 atggcacgtt actttctccc gaccatacgt tatcccctta cgccaaagaa actctgaagc
    27841 tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg tcgtcaccac gttgatgtgg
    27901 ggcaaattcg cgataatctg gagattaagt cttacatgat tacctccaat ggtgcgcgcg
    27961 ttcacgatct ggatggtaat ctgatttttg ctcataacct ggatcgcgac attgccagcg
    28021 atctgtttgg cgtagtcaac gacaatccgg acatcattac taacgtttat cgcgacgacg
    28081 aatggtttat gaatcgccat cgcccggaag agatgcgctt ttttaaagaa gcggtgttcc
    28141 aatatgcgct gtatgagcct ggattactgg agccggaagg cgtcagcaaa gtgttcttca
    28201 cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc gattaacgct cgttggggcg
    28261 atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga agtgatggcg ggcggcgttt
    28321 caaaaggcca tgcgctggaa gcggtggcga agaaactggg ctacagcctg aaggattgta
    28381 ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc gatggcgggg aaaggctgca
    28441 ttatgggcag tgcgcaccag cgtctgaaag accttcatcc cgagctggaa gtgattggta
    28501 ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact ctatttatcg taatcgttct
    28561 ttatttggtc agttgtcaac ctgatacttc gctacaatgg atacccgtta atcaaagagt
    28621 tttccattgt ggcgctactt atcatcacca cgattctgtg ggccttctcc tttagctttt
    28681 atggcgagta ccttgcgggg cacgtcgata gctattttgc ggtgctggtg cgcgttggcc
    28741 tggcggcact cgtttttctg ccgtttctgc gtacccgtgg caatagcctg aaaacagtcg
    28801 gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat gtatatgctg agtttccgcg
    28861 cttatctcta cctgacggtt tccgaactgc tgctgttcac cgtactgacg ccgctctaca
    28921 tcacgctgat ttatgacatc atgagtaagc gccgtctgcg ctggggatat gcttttagtg
    28981 ccttgttggc ggtgattggt gccgggatta ttcgctatga tcaggtcacc gaccatttct
    29041 ggactggctt gttgctggtg caactctcca atatcacttt tgccatcggc atggtgggtt
    29101 acaaacgcct gatggaaact cgcccgatgc cgcagcataa cgcctttgcg tggttctatc
    29161 ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct gggaaatacg cagaaaatgc
    29221 cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg cgtggtggct tccgggattg
    29281 gctactttat gtggaactac ggcgcgacgc aggtggacgc cggaacgctg ggcattatga
    29341 ataatatgca cgttccggca gggctgctgg taaacctggc tatctggcac caacagccgc
    29401 actggccaac gttcattaca ggcgcgctgg tgatcctggc ctcactgtgg gtgcatcgta
    29461 agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg caggcgtgat tgcgcgctga
    29521 gcgaataaac gcttccgtaa ctggctgacg ctgctctcca tcgcgcacgg cggcgtacag
    29581 tcggctccac aagccttcgc ccagggtttt ggtcaccacc agaccctggc gctcaaaact
    29641 ctctactacc caatgcggta gcgcggcaat acccatccgt gcggcaacca tctgaatcaa
    29701 caataaggtg ttatcgacgc ttttcagtga cgggctgacg cctgccggct gaagaaaatg
    29761 ccgccagaca tccagtcgac tacgctgtac cggataaatc aacagcgtct cgctggcgag
    29821 atcttccggt gtaattcgcg ttttcgccgc cagtggatgg tcaggtgcta acaccagacg
    29881 cacttcatag tcgaacatcg gcgaataatg caggccactg cgcggcagga tatcggaggt
    29941 catcaccaga tccagctcgc cctgttgcaa cgcgggctgc gggtcaaacg tcacgcccga
    30001 tttaaaatcc atctctacct gcggccagtt cttatggaaa ttttctaacg cgggcgtcag
    30061 ccactgaata cagctatggc actcaatggc aatgcgcaga cgcgtctgct gcggttcatt
    30121 gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg tttgccagtt gcaacaggat
    30181 ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc acaaatagcc ggaagccaag
    30241 gcgttgttcc agatcgctaa actggtgaga cagggcggat tgcgtctgat gcaacgtcgc
    30301 cgcagcggct gcgagagagc cgcagttccg caacgcttgt agcgttttca ggtgttttac
    30361 ttcgatcatg aaagtccttc acttcggcat gaataatttg cgcttgagga atatacagta
    30421 accgccaatt atggatgtgt aaacatctgg acggctaaat actaaaaatt ccataagggg
    30481 catataatga caattcttaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag
    30541 ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca cgcgtgaaga actgctgaca
    30601 gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg
    30661 ctgccggtgg gcgattttgc ctggtacgat catgtactga ccaccagttt gctgctgggt
    30721 aatgttccgc cacgtcatca gaacaaagac ggttcggtag atatcgacac cctgttccgt
    30781 attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg
    30841 tttaacacca actatcacta catggtgccg gagttcgtta aaggccaaca gttcaaactg
    30901 acctggacgc agctgctgga ggaagtggac gaggcgctgg cgctgggcca caacgttaag
    30961 cctgtgctgc tggggccggt tacttacctg tggctgggga aagtgaaagg tgaacagttt
    31021 gaccgcctta gcctgctgaa cgacattctg ccggtttatc agcaagtgct ggcagaactg
    31081 gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg cgctggtact ggaactacca
    31141 caggcgtggc tggacgcata caaacccgct tacgacgcgc tccagggaca ggtgaaactg
    31201 ctgctgacca cctattttga aggcgtaacg ccaaatctcg acacgattac tgcgctgcct
    31261 gttcagggtc tgcatgttga cctcgtacat ggtaaagatg acgttgctga actgcacaag
    31321 cgcctgcctt ctgactggtt gctgtctgcg ggtctgatca atggtcgtaa cgtctggcgc
    31381 gccgatctta ccgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg
    31441 gtggcatctt cctgctcgtt gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt
    31501 gatgcagaag tgaaaagctg gtttgccttc gccctacaaa aatgccatga actggcactg
    31561 ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg cagagtggag cgccccgatt
    31621 caggcacgtc gtcactctac ccgcgtacat aatccggcgg tagaaaagcg tctggcggcg
    31681 atcaccgccc aggacagcca gcgtgcgaat gtctatgaag tgcgtgctga agcccagcgt
    31741 gcgcgtttta aactgccagc gtggccgacc accacgattg gttccttccc gcaaaccacg
    31801 gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc tcgacgccaa caactaccgc
    31861 acgggcattg cggaacatat caagcaggcc attgttgagc aggaacgttt gggactggat
    31921 gtgctggtac atggcgaggc cgagcgtaat gacatggtgg aatactttgg cgagcacctc
    31981 gacggatttg tctttacgca aaacggttgg gtacagagct acggttcccg ctgcgtgaag
    32041 ccaccgattg tcattggtga cattagccgc ccggcaccga ttaccgtgga gtgggcgaag
    32101 tatgcgcaat cgctgaccga caaaccggtg aaagggatgc tgacggggcc ggtgaccata
    32161 ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa ccatcgccaa acagattgcg
    32221 ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg gaattggcat catccagatt
    32281 gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta gcgactggga tgcgtatctc
    32341 cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc
    32401 cacactcaca tgtgttattg cgagttcaac gacatcatgg attcgattgc ggcgctggac
    32461 gcagacgtca tcaccatcga aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa
    32521 gagtttgatt atccaaatga aatcggtcct ggcgtctatg acattcactc gccaaacgta
    32581 ccgagcgtgg aatggattga agccttgctg aagaaagcgg caaaacgcat tccggcagag
    32641 cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg
    32701 gcactggcga acatggtgca ggcggcgcag aacttgcgtc gggggtaaaa tccaaaccgg
    32761 gtggtaatac cacccggtct tttctcatta cagcgacttc ttcccaccat actgcttaaa
    32821 ccattccagc atacgctgcc agccatcttc tgcagatgcg gcatgatagc tcgggcgata
    32881 atcagcgttg aatgcatgcc cggcgtccgg gtacacgata atctctgctt tcgcattagc
    32941 agcccgcagc gcctggcgca tggtttcaac gctctcctgc ggaatgctgt tatcctgacc
    33001 accatataag ccgagaatcg gcgcgttaag atcggttgcg atatcaacag gttgtttcgg
    33061 tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac gccactgcgg cttttagctg
    33121 tggattatgc gcggcataca gccaggtgat acgtccaccc cagcagaatc cggtgatcat
    33181 taaacgatga acatcgccgc cgttgcggga agcccaactg gcgacatgat cgagatcggc
    33241 cagcacctgc gagtcaggca cttttgctac cagaccgcta agcaatgtgg ggatatcggc
    33301 aaaatcattc ggatcgcctt cgcggaagta aagttcaggt gcgatagcca gatacccctc
    33361 cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac
    33421 tacaatgacc actggcagtg ggccatcgct ttgctttggt ctggcatggt aagcaggcat
    33481 gttatcccct tgtgaaggga tagaggtgaa gcccgccaca atcgcgtcgt ccggggtctg
    33541 aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc
    33601 catggtattc tccgtaccct tataaaaatg ttgcgcaatg ttaactatag tcagcatgca
    33661 acaaatcaca ttgcctgaat cggctcatct tttatgcagt cctgcagaat gaagggtgat
    33721 ttatgtgatt tgcatcactt ttggtgggta aatttatgca acgcatttgc gtcatggtga
    33781 tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt ctttttgctt cttctgacta
    33841 aaccgattca cagaggagtt gtatatgtcc aagtctgatg tttttcatct cggcctcact
    33901 aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa
    33961 aagatcgccg cgctgatgga taagccggtt aagctggcat ctcaccgcga attcactacc
    34021 tggcgtgcag agctggatgg taaacctgtt atcgtctgct ctaccggtat cggcggcccg
    34081 tctacctcta ttgctgttga agagctggca cagctgggca ttcgcacctt cctgcgtatc
    34141 ggtacaacgg gcgctattca gccgcatatt aatgtgggtg atgtcctggt taccacggcg
    34201 tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct
    34261 gatttcgaat gtacgactgc gctggttgaa gctgcgaaat ccattggcgc gacaactcac
    34321 gttggcgtga cagcttcttc tgataccttc tacccaggtc aggaacgtta cgatacttac
    34381 tctggtcgcg tagttcgtca ctttaaaggt tctatggaag agtggcaggc gatgggcgta
    34441 atgaactatg aaatggaatc tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt
    34501 gccggtatgg tagcgggtgt tatcgttaac cgcacccagc aagagatccc gaatgctgag
    34561 acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg tggaagcggc gcgtcgtctg
    34621 ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag
    34681 cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca gcacaattct attttgtgcg
    34741 ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat catggtttac gcagttattg
    34801 cgttggtggg tgtggcaatt ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag
    34861 ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa
    34921 ttacccaaag cgagcactgg cgtgcagagt gcgagttact caataacgaa gtgcgcagcc
    34981 tgcaaagtat taacacctct ctggaggccg atctgcgtga agtaaccacg cggatggaag
    35041 ccgcacagca acatgctgac gataaaattc gccagatgat taatagcgag cagcgcctca
    35101 gtgagcagtt tgaaaacctc gccaaccgta tttttgagca cagcaatcgc cgggttgatg
    35161 agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt
    35221 tccgccgtca ggttcaggac agcttcggta aagaagcaca agaacgccat accctgaccc
    35281 acgaaattcg caatctccag caactcaacg cgcaaatggc ccaggaagcg atcaacctga
    35341 cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg gggcgaggta gtattgacgc
    35401 gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg
    35461 aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt gcgcctgccg cagggaaaag
    35521 atgtggtgat cgacgccaaa atgacgctgg tcgcctatga acgctatttt aacgccgaag
    35581 acgactacac ccgcgaaagc gcgctacagg aacatatcgc gtcggtgcgt aaccatatcc
    35641 gtttgctggg acgcaaagat tatcaacagc tgccggggct gcgaactctg gattacgtgc
    35701 tgatgtttat tcccgttgaa cccgcttttt tactggcgct tgaccgccag ccggagctga
    35761 tcaccgaagc gttgaaaaac aacatcatgc tggttagccc gactacgctg ctggtggcgc
    35821 tgcgcactat cgccaacctg tggcgttatg agcatcaaag ccgcaacgcc cagcaaatcg
    35881 ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt catcgatgac atgtccgcga
    35941 ttggtcaaag tctcgacaaa gcgcaggata attatcggca ggcaatgaaa aaactctctt
    36001 cagggcgcgg aaatgtgctg gcgcaggcag aagcgtttcg cggtttagga gtagaaatta
    36061 aacgcgagat taatccggat ttggctgaac aggcggtgag ccaggatgaa gagtatcgac
    36121 ttcggtcggt tccggagcag ccgaatgatg aagcttatca acgcgatgat gaatataatc
    36181 agcagtcgcg ctagcccatt gggagtagtt aagccgggta gaaatctagg gcatcgacgc
    36241 ccaatctgtt acacttctgg aacaattttt tgatgagcag gcattgagat ggtggataag
    36301 tcacaagaaa cgacgcactt tggttttcag accgtcgcga aggaacaaaa agcggatatg
    36361 gtcgcccacg ttttccattc cgtggcatca aaatacgatg tcatgaatga tttgatgtca
    36421 tttggtattc atcgtttgtg gaagcgattc acgattgatt gcagcggcgt acgccgtggg
    36481 cagaccgtgc tggatctggc tggtggcacc ggcgacctga cagcgaaatt ctcccgcctg
    36541 gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg aatccatgct caaaatgggc
    36601 cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg agtatgttca ggcgaacgct
    36661 gaggcgctgc cgttcccgga taacaccttt gattgcatca ccatttcgtt tggtctgcgt
    36721 aacgtcaccg acaaagataa agcactgcgt tcaatgtatc gcgtgctgaa acccggcggc
    36781 cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc cgctgagcaa agcctatgat
    36841 gcatactcct tccatgtgct gccgcgtatt ggctcactgg tcgcgaacga cgccgacagc
    36901 taccgttatc tggcagaatc catccgtatg catcccgatc aggataccct gaaagccatg
    36961 atgcaggatg ccggattcga aagtgtcgac tactacaatc tgacggcagg ggttgtggcg
    37021 ctgcatcgtg gttataagtt ctgacaggag accggaaatg ccttttaaac ctttagtgac
    37081 ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc tcacccgcgc tgaaaacggc
    37141 ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta aaaggctttt cgacgtcatt
    37201 gattctggtg ttcagcgaac gccaggttga tgtactgggc gaatgggcag gcgatgctga
    37261 ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt cgcgatcgcc agcagcttac
    37321 cgcactgatt cgcagtggtg agctggaagt gcagggcgat attcaggtgg tgcaaaactt
    37381 cgttgcgctg gcagatctgg cagagttcga ccctgcggaa ctgctggccc cttataccgg
    37441 tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga ggcgcaaagt tcctgcatca
    37501 cggcattaag cgccagcaac gttatgtggc ggaagccatt actgaagagt ggcgtatggc
    37561 acccggtccg cttgaagtgg cctggtttgc ggaagagacg gctgccgtcg agcgtgctgt
    37621 tgatgccctg accaaacggc tggaaaaact ggaggctaaa tgacgccagg tgaagtacgg
    37681 cgcctatatt tcatcattcg cactttttta agctacggac ttgatgaact gatccccaaa
    37741 atgcgtatca ccctgccgct acggctatgg cgatactcat tattctggat gccaaatcgg
    37801 cataaagaca aacttttagg tgagcgacta cgactggccc tgcaagaact ggggccggtt
    37861 tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc tttttccacc gcatattgcc
    37921 gatcagctgg cgttattgca ggacaaagtt gctccgtttg atggcaagct ggcgaagcag
    37981 cagattgaag ctgcaatggg cggcttgccg gtagaagcgt ggtttgacga ttttgaaatc
    38041 aagccgctgg cttctgcttc tatcgcccag gttcataccg cgcgattgaa atcgaatggt
    38101 aaagaggtgg tgattaaagt catccgcccg gatattttgc cggttattaa agcggatctg
    38161 aaacttatct accgtctggc tcgctgggtg ccgcgtttgc tgccggatgg tcgccgtctg
    38221 cgcccaaccg aagtggtgcg cgagtacgaa aagacattga ttgatgaact gaatttgctg
    38281 cgggaatctg ccaacgccat tcagcttcgg cgcaattttg aagacagccc gatgctctac
    38341 atcccggaag tttaccctga ctattgtagt gaagggatga tggtgatgga gcgcatttac
    38401 ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg gcactaacat gaaattgctg
    38461 gcggaacgcg gcgtgcaggt gttcttcact caggtctttc gcgacagctt tttccatgcc
    38521 gatatgcacc ctggcaacat cttcgtaagc tatgaacacc cggaaaaccc gaaatatatc
    38581 ggcattgatt gcgggattgt tggctcgcta aacaaagaag ataaacgcta tctggcagaa
    38641 aactttatcg ccttctttaa tcgcgactat cgcaaagtgg cagagctaca cgtcgattct
    38701 ggctgggtgc caccagatac caacgttgaa gagttcgaat ttgccattcg tacggtctgt
    38761 gaacctatct ttgagaaacc gctggccgaa atttcgtttg gacatgtact gttaaatctg
    38821 tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc aactggtgtt actccagaaa
    38881 accctgctct acgtcgaagg ggtaggacgc cagctttatc cgcaactcga tttatggaaa
    38941 acggcgaagc ctttcctgga gtcgtggatt aaagatcagg tcggtattcc tgcgctggtg
    39001 agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa tgccagaact gcctgaattg
    39061 gtttacgaca gtttgcgcca gggcaagtat ttacagcaca gtgttgataa gattgcccgc
    39121 gagcttcagt caaatcatgt acgtcaggga caatcgcgtt attttctcgg aattggcgct
    39181 acgttagtat taagtggcac attcttgttg gtcagccgac ctgaatgggg gctgatgccc
    39241 ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg gttggcgcaa aacacgctga
    39301 ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc ggctttgttt aatcatcatc
    39361 taccacagag gaacatgtat gggtggtatc agtatttggc agttattgat tattgccgtc
    39421 atcgttgtac tgctttttgg caccaaaaag ctcggctcca tcggttccga tcttggtgcg
    39481 tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac caaagcagga taaaaccagt
    39541 caggatgctg attttactgc gaaaactatc gccgataagc aggcggatac gaatcaggaa
    39601 caggctaaaa cagaagacgc gaagcgccac gataaagagc aggtgtaatc cgtgtttgat
    39661 atcggtttta gcgaactgct attggtgttc atcatcggcc tcgtcgttct ggggccgcaa
    39721 cgactgcctg tggcggtaaa aacggtag
//
LOCUS       DACAPB010000018        39151 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.018, whole genome shotgun sequence.
ACCESSION   DACAPB010000018 DACAPB010000000
VERSION     DACAPB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.018"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(103..456)
                     /gene="hlyE"
                     /locus_tag="IT904_000861"
                     /pseudo
     CDS             complement(103..456)
                     /gene="hlyE"
                     /locus_tag="IT904_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            829..1248
                     /gene="umuD"
                     /locus_tag="IT904_000862"
     CDS             829..1248
                     /gene="umuD"
                     /locus_tag="IT904_000862"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP3190688.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1248..2516
                     /gene="umuC"
                     /locus_tag="IT904_000863"
     CDS             1248..2516
                     /gene="umuC"
                     /locus_tag="IT904_000863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HAP3190689.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(2562..3092)
                     /gene="dsbB"
                     /locus_tag="IT904_000864"
     CDS             complement(2562..3092)
                     /gene="dsbB"
                     /locus_tag="IT904_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAP3190690.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(3238..4779)
                     /gene="nhaB"
                     /locus_tag="IT904_000865"
     CDS             complement(3238..4779)
                     /gene="nhaB"
                     /locus_tag="IT904_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAP3190691.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            5000..5719
                     /gene="fadR"
                     /locus_tag="IT904_000866"
     CDS             5000..5719
                     /gene="fadR"
                     /locus_tag="IT904_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAP3190692.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5771..7303)
                     /locus_tag="IT904_000867"
     CDS             complement(5771..7303)
                     /locus_tag="IT904_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAP3190693.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7633..8931
                     /locus_tag="IT904_000868"
     CDS             7633..8931
                     /locus_tag="IT904_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAP3190694.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8941..10011
                     /gene="dadX"
                     /locus_tag="IT904_000869"
     CDS             8941..10011
                     /gene="dadX"
                     /locus_tag="IT904_000869"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAP3190695.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(10397..12133)
                     /locus_tag="IT904_000870"
     CDS             complement(10397..12133)
                     /locus_tag="IT904_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAP3190696.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQHHESNISWMFIVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFSLTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12228..13142)
                     /gene="ldcA"
                     /locus_tag="IT904_000871"
     CDS             complement(12228..13142)
                     /gene="ldcA"
                     /locus_tag="IT904_000871"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAP3190697.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13242..13853
                     /gene="emtA"
                     /locus_tag="IT904_000872"
     CDS             13242..13853
                     /gene="emtA"
                     /locus_tag="IT904_000872"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAP3190698.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13855..14589)
                     /locus_tag="IT904_000873"
     CDS             complement(13855..14589)
                     /locus_tag="IT904_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAP3190699.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14790..15044
                     /locus_tag="IT904_000874"
     CDS             14790..15044
                     /locus_tag="IT904_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP3190700.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            15222..15662
                     /locus_tag="IT904_000875"
     CDS             15222..15662
                     /locus_tag="IT904_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190701.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15741..17438)
                     /locus_tag="IT904_000876"
     CDS             complement(15741..17438)
                     /locus_tag="IT904_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAP3190702.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTGIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17758..19176)
                     /gene="dhaM"
                     /locus_tag="IT904_000877"
     CDS             complement(17758..19176)
                     /gene="dhaM"
                     /locus_tag="IT904_000877"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAP3190703.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLATEIATKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(19187..19819)
                     /gene="dhaL"
                     /locus_tag="IT904_000878"
     CDS             complement(19187..19819)
                     /gene="dhaL"
                     /locus_tag="IT904_000878"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAP3190704.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19830..20900)
                     /gene="dhaK"
                     /locus_tag="IT904_000879"
     CDS             complement(19830..20900)
                     /gene="dhaK"
                     /locus_tag="IT904_000879"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP3190705.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVTG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            21119..23047
                     /gene="dhaR"
                     /locus_tag="IT904_000880"
     CDS             21119..23047
                     /gene="dhaR"
                     /locus_tag="IT904_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAP3190706.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            complement(23147..26014)
                     /locus_tag="IT904_000881"
     CDS             complement(23147..26014)
                     /locus_tag="IT904_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP3190707.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYSVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMTMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(26783..27874)
                     /gene="ychF"
                     /locus_tag="IT904_000882"
     CDS             complement(26783..27874)
                     /gene="ychF"
                     /locus_tag="IT904_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP3190708.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27991..28575)
                     /gene="pth"
                     /locus_tag="IT904_000883"
     CDS             complement(27991..28575)
                     /gene="pth"
                     /locus_tag="IT904_000883"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP3190709.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28853..29131
                     /gene="ychH"
                     /locus_tag="IT904_000884"
     CDS             28853..29131
                     /gene="ychH"
                     /locus_tag="IT904_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAP3190710.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(29186..30865)
                     /gene="dauA"
                     /locus_tag="IT904_000885"
     CDS             complement(29186..30865)
                     /gene="dauA"
                     /locus_tag="IT904_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAP3190711.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30990..31937)
                     /locus_tag="IT904_000886"
     CDS             complement(30990..31937)
                     /locus_tag="IT904_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAP3190712.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(32088..32939)
                     /gene="ispE"
                     /locus_tag="IT904_000887"
     CDS             complement(32088..32939)
                     /gene="ispE"
                     /locus_tag="IT904_000887"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP3190713.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32939..33562)
                     /gene="lolB"
                     /locus_tag="IT904_000888"
     CDS             complement(32939..33562)
                     /gene="lolB"
                     /locus_tag="IT904_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAP3190714.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33776..35032
                     /gene="hemA"
                     /locus_tag="IT904_000889"
     CDS             33776..35032
                     /gene="hemA"
                     /locus_tag="IT904_000889"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAP3190715.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            35074..36156
                     /gene="prfA"
                     /locus_tag="IT904_000890"
     CDS             35074..36156
                     /gene="prfA"
                     /locus_tag="IT904_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP3190716.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            36156..36989
                     /gene="prmC"
                     /locus_tag="IT904_000891"
     CDS             36156..36989
                     /gene="prmC"
                     /locus_tag="IT904_000891"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP3190717.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36986..37378
                     /locus_tag="IT904_000892"
     CDS             36986..37378
                     /locus_tag="IT904_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAP3190718.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            37382..38191
                     /locus_tag="IT904_000893"
     CDS             37382..38191
                     /locus_tag="IT904_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAP3190719.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            38227..39081
                     /gene="kdsA"
                     /locus_tag="IT904_000894"
     CDS             38227..39081
                     /gene="kdsA"
                     /locus_tag="IT904_000894"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAP3190720.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         9762 a         9724 c        10038 g         9627 t
ORIGIN      
        1 ccttcagtag cggagcagga aggatatctg atggaaatga aagccacgca agcaccttaa
       61 aatcaccatc atacactaaa tcagtaagtt ggcagcatta cccttaatga gaatgtcttt
      121 ctgggcggat gctttcttct cattgtactc atcaaatagc aaaatatacg ctgcgagcaa
      181 ttgcgtcgca acaccacacc attcatacac tgtttgggtt gcttcaaaat acttatcctg
      241 gctatccata agtaaggttt taatatcgcc gactaaaacg gaggctgcct gtgaatactc
      301 ctgtttaaag cgacttaact cttttatggt ttcatcaaag gtctgccagg ggatgacctg
      361 atcgagatat ttattataaa gatctaatgc tccatctgcg gtttcgattg cgtttttaac
      421 tacttctacc gttttatctg caacgatttc agtcataatc attcgcctct ttaaatatat
      481 aaattgtaat gaaactcctg ttttacaact attaataaat tttacttcat ctaattcata
      541 gttagccggg cgggatgcgt caatgtcttt atttctatta atatgataaa tatcaaacaa
      601 tgtttaatgt cattatggcg aatgcttcta ttctattttt ttgccgggtg atatttttca
      661 tttctgctgg atgagcgtcg tcgccagaag gccacgtgag cacaggataa gagaacgaaa
      721 aatcagcagt ctatgcagcg acaaatattg atagcctgaa tcagtattga tctgctggca
      781 agaacagact actgtatata aaaacagtat aacttcaggc agattattat gttgtttatc
      841 aagcctgcgg atctccgcga aattgtgact tttccgctat ttagcgatct tgttcagtgt
      901 ggctttcctt caccggcagc agattacgtt gaacagcgca tcgatctgaa tcaactgttg
      961 atccagcatc ccagcgcgac ttacttcgtc aaagcaagtg gtgattctat gattgatggt
     1021 ggaattagtg acggtgattt actgattgtc gatagcgcta ttaccgccag ccatggtgat
     1081 attgtcatcg ctgctgttga cggcgagttt acggtgaaaa aattgcaact acgcccgacg
     1141 gtacagctta ttcccatgaa cagtgcgtac tcgcccatta ccatcagtag cgaagatacg
     1201 ctggatgtct ttggtgtggt gatccacgtc gttaaggcga tgcgctgatg tttgctctct
     1261 gtgatgtaaa cgcgttttat gccagctgtg agacggtgtt tcgccctgat ttatggggta
     1321 aaccggtggt tgtgctatcg aataatgacg gttgcgttat cgcccgaaac gctgaggcaa
     1381 aggcgctggg tgttaaaatg ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct
     1441 gtggtgtggt ttgctttagc agcaattatg agctttacgc agacatgagc aatcgggtga
     1501 tgtcgacgct ggaagagcta tcgccccgcg tcgagattta cagtattgat gaggcattct
     1561 gcgatctgac aggtgtgcgt aattgtcgcg atctgactga ttttggcaga gaaattcgcg
     1621 caacggtgct acaacgtacc catcttactg ttggcgtggg gatcgcccag accaaaacgc
     1681 tggctaagct tgccaatcat gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg
     1741 atttatcaaa tctggaacgc cagcgtaaat taatgtctgc tctccccgtg gatgacgtct
     1801 gggggattgg acggcggatc agcaaaaaac tggacgcgat ggggatcaaa accgttctcg
     1861 atttggcgga tacagatatc cggtttatcc gtaaacattt taatgtcgtg ctcgaaagaa
     1921 cggtgcgtga actgcgcggc gaaccctgtt tgcaactgga agagtttgca ccgacgaagc
     1981 aggaaattat ctgttcccgc tcgtttggtg aacgcatcac ggattatccg tcgatgcggc
     2041 aggccatttg tagttacgct gcccgggcgg cggaaaaact tcgcagcgag catcaatatt
     2101 gtcggtttat ctccacgttt attaagacgt caccatttgc gctcaatgaa ccttattacg
     2161 gcaatagcgc gtcggtaaaa ctgctgacgc ccactcagga cagcagggat atcattaacg
     2221 ctgctacgcg atctctggat gccatctggc aagcgggcca tcgttaccaa aaagcgggcg
     2281 tgatgctggg ggatttcttc agtcagggag tcgcgcagct caatttattc gatgacaacg
     2341 caccgcgccc cgggagtgag caattgatga cggtaatgga tacactgaat gctaaagagg
     2401 gcagaggaac actctatttt gccgggcagg ggatccagca acaatggcag atgaagcgag
     2461 ccatgctttc accacgttat acaacgcgaa gttctgattt actgagggtc aaataaatat
     2521 agcggcagga aaaaagcgct cccgcaggag cgccgaatgg attagcgacc gaacagatca
     2581 cgttttttcg ctttaaacgg ctgggaaatc accaccagca ctgcgacaat caggtaagcg
     2641 ataaaaatac cgagcagcca ctgcggcatt tccagaccta aaaaatccca ctgacgctcg
     2701 gcgcaatcgc cagaggcgac aaacacttgc ggcacccact tatccagcgg cagccattcc
     2761 gggaaacgaa ccataaaatc acaggtggca aacggcgaag gatagagctg aagcatggtg
     2821 tgctcgtaag ttaactgcac accgcggaac gcactataca accagataac catcgctaca
     2881 taacgcagcg gagttttcgg ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat
     2941 aacgcgcagc gttcataaat acagagcacg caaggtttca gtaacatcac atgctggaac
     3001 cacagcgccg tcagttccag tgccagagca gtaaacgcca tcaacagcca cgcgccccgg
     3061 ccttgtgaac attggttcaa aaatcgcaac ataatcattt ccctgcaata tgcatagagt
     3121 gcgcagttta aaccaattca ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat
     3181 tggacaatta tcgggcgaaa aggcaaaccg ggcatttata tgcccggtaa gttgttatca
     3241 aagcgttgct atccagccca tttgcataaa ccattcggtt acaggggcaa gcgtaaactc
     3301 gacgcagagc aagccgacga gtgtcaggac gagggtgtaa ggcagggcca tccacaccat
     3361 gcggccataa gagaggcgaa tcaatggcgc gagtgcagag gtcagcagga acaggaacgc
     3421 agcctgaccg ttcggcgtag cgacggaggg cagattggta ccggtattaa tggcgaccgc
     3481 cagcagctcg tattgcttca acgtgatagc gccactttcc attgccgctt tcgcttcgtt
     3541 gatataaatc gtccccacga agacgttatc cgaaatggat gacagcagac cgttgaaaat
     3601 atagaacagc gacagctgag catgttccga tgcctgcaac acaaactgaa taattggcga
     3661 aaacagttgt tggtcgataa tcaccgcgac taccgagaaa aacaccgtca acagtgcggt
     3721 gaatggcaga gattcggtga aggctttacc gatagcatgc tcatcggtga caccggtcaa
     3781 tgatgttgcc agaataatga ctgacaaacc aatcaagcca acttccgcca gatgcaacgc
     3841 cagcgcagtc accagccaga cgccaataat cgcctggaca atcagacgga ttttatcctg
     3901 acgggtacgc tggtggcggc tttgatcgtc aaactgttgc aacacttcgc ggactttctc
     3961 cggcagcgtt tcaccgtagc caaaccaacg cagcttctct accagcaggc aggttaacag
     4021 gccacaaatc agaaccggaa cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg
     4081 ccagccagcc gctttagcga tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc
     4141 gcctaatgcg gtgccgacac cggcatgcat catcaggcta cgcagaaagc cacggaactg
     4201 ttccagaacc actttgtaat gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc
     4261 ggtacgggaa gaggctacgc ggtgataaat accataaaaa ccgactgcaa cgctgatcac
     4321 caccgccacg acggttaagg catcgaggaa cgcggagagg aacgcagccg ccacgcaaaa
     4381 agagagcgac agcagcattt tggagcgaat gcttaacagc aaacgggtaa atatgaacag
     4441 caacagctgt ttcataaaat agatacccgc caccataaac atcagtaaca gcaagacttc
     4501 aagatttgcc gccacctctt cacggacgtg ttccgcgctg gtcatgccga tgaataccgc
     4561 ttcgatagcc aacagaccac cggggagcag cgggtagcat ttcagggcca tcgccagagt
     4621 gaaaataaat tccgctacca gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa
     4681 cgggtttacg attaagaaaa tgatgagggc gagtttgtac cagtcggggg actggcccaa
     4741 aaaattgcgc catagcgcgc ggccccagga gatctccatg atggtttccc ttaccttaca
     4801 aataatcaat gatgttttta tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc
     4861 aagtcttgat agtcaagggg aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc
     4921 tttttgtctg ctatcagcgt agttagccct ctggtatgat gagtccaact ttgttttgct
     4981 gtgttatgga aatctcacta tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga
     5041 gtacattatt gaaagtatct ggaataaccg cttccctccc gggactattt tgcccgcaga
     5101 acgtgaactt tcagaattaa ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg
     5161 tctggcacga gatggctggt tgaccattca acatggcaag ccgacgaagg tgaataattt
     5221 ctgggaaact tccggtttaa atatccttga aacactggcg cgactggatc acgaaagtgt
     5281 gccgcagctt attgataatt tgctgtcggt gcgtaccaat atttccacta tttttattcg
     5341 caccgcgttt cgtcagcatc ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt
     5401 ggccgatcac gccgatgcct ttgccgagct ggattacaac atattccgcg gcctggcgtt
     5461 tgcttccggc aacccgattt acggtctgat tcttaacggg atgaaagggc tgtatacgcg
     5521 tattggtcgt cactatttcg ccaatccgga agcgcgcagt ctggcgctgg gcttctacca
     5581 caaactgtcg gcgttgtgca gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg
     5641 ctatgggcat gagagtggcg agatttggca ccggatgcag aaaaatctgc cgggtgattt
     5701 agccattcag gggcgataat cccttctgtt taaagagcaa acccctcaaa cgaggggttt
     5761 tttgttgttt ttacagattt cccattcttg gcgggcaacg ttccagcaac tcgatgctgc
     5821 cgtcttcgtt ttgctgttcg agcatcacat caaatcccca caggcgatgc acatgcttca
     5881 ggacttcttt gcgcccccga tccagcggtg cgcgattatg tggaatataa cgcagcgtca
     5941 gcgaacggtc gccgcgcaaa tccacgttcc agatctgaat attcggctcc agattactta
     6001 agttatattg cgacgataac cggttacgga tctcccgata accttcttca ttatgaatag
     6061 cggaaatctc cagataatta tgccgatcgt catccagcac ggtgaagaag cggaaatcac
     6121 gcatcacttt cggtgacagg aactggctga taaaactctc atctttgaaa tcacgcatcg
     6181 cgaaatgcag cgtttccagc cagtcggaac cggcgatatc cgggaaccag tatttgtctt
     6241 cttccgttgg cgactgacaa atccgtttaa tatcctggaa catggcgaac ccgagggcat
     6301 acgggttgat gccgctgtac cacgggctgt tatagggggg ctggaagacc acattggtgt
     6361 ggctgtgcaa aaactccagc ataaaacgtt ccgttacttt cccttcatca tacagatggt
     6421 taaggatggt gtagtgccag aaggtcgccc agccttcgtt catcacctga gtctgttttt
     6481 gcggataaaa atactggctc accttacgca caatacgcag gatttcacgc tgccatgatt
     6541 ccagcagtgg cgcatttttc tccataaaat agagcaggtt ttcttgtggt tcggacggat
     6601 agcggcgcgc ttcagcaacc gttttctctt cctcgcgctt cggcagggta cgccagagca
     6661 tattgacctg actttgcaga tactcttcgc gacttttctg ccgggctttc tcttcttgca
     6721 gcgagatttt ttgcgggcgt ttgtaccgat ccacgccgta gttcatcagc gcgtggcacg
     6781 agtccagaag ccgttctact tcatcaacgc cataacgctc ttcgcactcg gtaatatatt
     6841 tacgggcaaa aatcagataa tcgacaatcg aactggcgtc ggtccagcta cggaataagt
     6901 aattgttttt gaagaaagag ttatgcccat agcaggcatg agccatcacc agcgcttgca
     6961 tggtaatggt gttctcttcc atcaggtaag cgatacacgg gttagagtta atgacgattt
     7021 cataggccag tccttgctga ccgtgcttat acagccgttc agtctcgata aactttttac
     7081 cgaatgacca gtgcggatag ttaattggca tgccgacgct ggagtaggca tccatcatct
     7141 gttctgaggt tatcacttca atctggtgcg ggtaggtatc cagccggtag agtttcgcca
     7201 cccggtctat ctctgccaga taaacatcca gcagatcgaa cgtccagtcg ggtccatcgc
     7261 tcaaacgtgt ggtgtcctta ttcatagaat cgatcgtcgc catacgcgca cctcattgtt
     7321 gtcggcgctc tctgtgtgga gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg
     7381 tcgccggaga atttttttct ttgcgatttc ttattatcag agtgccacta atccgcttct
     7441 gaacggaatt ttatgctgga taaaaagggc gttcagcagg agatactaaa gacgccatat
     7501 tgccgcagag tcagggagat gtgagccagc tcaccataaa aaagccgtat gttgaataat
     7561 attttcaact gagttatcaa gatgtgatta gattattatt cttttactgt atctaccgtt
     7621 atcggagtgg ctatgcgagt tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc
     7681 tggtacttaa atcaggcagg acatgaggtc accgtcattg atcgggagcc gggggccgcc
     7741 ctggaaacca gtgccgcgaa tgccgggcaa atctcccccg gatatgctgc accgtgggcg
     7801 gcaccaggtg tgcctttaaa agcgattaaa tggatgttcc agcgccatgc gccgctggcg
     7861 gttcgtctcg acggtacgca gttccagttg aaatggatgt ggcaaatgtt acgtaactgc
     7921 gacaccagcc actacatgga aaacaaaggg cggatggtgc gtctggcgga atacagccgt
     7981 gattgcctga aagcattgcg cgccgaaacc aatattcagt atgaagggcg tcagggtggg
     8041 acgctgcaac tgttccgtac cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg
     8101 ctggaagatg ccggcgtacc gtatcagctg ctggaatcca gccgcctggc ggaagtggag
     8161 cccgcgctgg cagaagtggc gcacaaactg acgggcggcc tgcagttacc caatgatgaa
     8221 accggagact gtcagctatt tacccagaat ctggcgcgga tggcggagca ggcgggggtt
     8281 aaattccgct ttaatacgcc cgttgaccaa ctgctttgcg acggcgagca aatctacggc
     8341 gtgaagtgtg gcgatgaagt gattaaggcc gatgcgtatg tgatggcgtt tggttcttac
     8401 tcgacggcga tgctcaaagg cattgttgat attccggtgt atccgctgaa aggctactcg
     8461 ctgaccattc caattgcgca ggaagatggt gcgccggtat ccaccattct tgatgaaacc
     8521 tacaaaatcg ccattacccg tttcgataac cgcattcgtg ttggcggaat ggcggagatt
     8581 gttggtttta ataccgagct gttgcaaccg cgtcgtgaaa cgctggagat ggtggttcgc
     8641 gatctctatc cacgcggcgg tcatgtcgag caggcgactt tctggactgg tctgcgcccg
     8701 atgacgccag acggcacgcc ggttgtcggg cgtacacgct ttaaaaatct gtggctgaat
     8761 accggtcacg gcacgctcgg ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat
     8821 ctgctctctg gtcgcacgcc agcgatccca tatgaggatc taagcgtagc gcgctacagc
     8881 cgtggattta cgccatcacg tccgggccat ttacatggcg cacacagcta aggaaacgag
     8941 atgacccgtc cgatacaggc cagcctcgat ctgcaggcat taaaacagaa tctgtccatt
     9001 gtccgccagg ccgcgacgca cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg
     9061 catggtattg agcgtatctg gagcgcgctc ggggccaccg atggctttgc attgcttaac
     9121 ctggaagagg caataacgtt acgtgagcgc ggctggaaag gaccgatcct gatgctggaa
     9181 ggatttttcc atgctcagga tctggagatt tatgaccagc accgcctgac cacctgcgta
     9241 cacagcaact ggcagctcaa agcactgcaa aatgcgcggc taaaagcacc gttggatatt
     9301 tatcttaaag tgaacagtgg gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc
     9361 gtctggcagc agttgcgggc aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt
     9421 gccgaagcgg aacatcctga tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg
     9481 gaggggctgg agtgtcggcg ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa
     9541 gcgcattttg actgggttcg gcctggcatt attttgtatg gcgcttcgcc gtccggtcag
     9601 tggcgtgata tcgccaatac cggattacgt ccggtgatga cgctaagcag tgagattatt
     9661 ggtgtccaga cgctaaaagc gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc
     9721 gatgaacagc gaatcggcat tgtcgccgca gggtacgccg acggttatcc gcgccacgcg
     9781 cctaccggta cccctgtttt agtggacggc gtgcgcacca tgacggtggg gaccgtctcg
     9841 atggatatgc tagcggtcga tttaacgcct tgcccgcagg cgggtattgg tacgccggtt
     9901 gagctgtggg gcaaggagat caaaattgat gatgtcgccg ccgctgccgg aacggtgggc
     9961 tatgagttga tgtgcgcgct ggcgctacgc gtcccggttg tgacggtgta acttgttgta
    10021 agccggatcg gaggcaacgt cttctgggtg caaaaaaatc atccatccgg ctggtcagca
    10081 actgtagttg ttaatgtgac agagccattg cccatgatag tgtccattaa aaggatggac
    10141 actatttccc cggaacctga actcaccgca caggcgttct acataaaacg cttacgcttc
    10201 attgttgact cgaactcgac ttcagataaa tcacgcttta cccttgatgg agcctgtaca
    10261 tagatttgtg taattgcctg attttgatat gttcaattca acatcaaatg aaggttaaat
    10321 tatggacgac aaacaattgc aggctcaggc tgcgttcagc aaagcatcgc aaccggcgat
    10381 agatgcttca ttaaatttaa gattcagctt cctcttcagc cacccgtacg ccaatcttca
    10441 acacttcatt atcttctttc tcggccaccg tccagatcat cccggcaaac tctacctggt
    10501 caccgacaac cggtgccgcg cctaacaact gctggacaat ttcacccagc gtttgctgct
    10561 tatcacgata ttctcgcccg tcttccaggc catatatcag cgcaacatca gcatatttgg
    10621 cgctggcttc gagaatgaag tcaccaaaga agcgttgatc cagcgcgacc ggcggcgact
    10681 ggctgaacag tttaccgagc gccggaagat cgcgttcccg accaattaca cacaacacat
    10741 cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa ctggttatca cgaaacagtg
    10801 ccgcaatacg cgtctctttt ggcatatgca aatcgcgcag tgccgcgccc acgcaccatt
    10861 tatcggcact caattgataa acaaactgct cccacggatt ttccggatga atatccaggc
    10921 caacgcgtga caccggacgt cccactggcg gaaccaccac tttggctttt ttagccgccc
    10981 acgagagtga tgttccctgc aagagcagtg aaaccagaac cacaaagaag gcgacattaa
    11041 agaacagacg tgcattctcc agccccgcca tcatcgggaa cacggccagg atgattggca
    11101 ccgcgccgcg taatcctacc cagctgataa acacgcgctc acgcagattg aaaccgcgga
    11161 aggggagcaa tccagcaaat accgaaaggg gacgggcgaa gaatatcatc catgcggaca
    11221 aaatgagcgc cggaatggca ataggcagca gatcgcttgg gttaaccaac agccccagca
    11281 ccaggaacat ggcgatttgc gccagccagg cgaggccgtc gaaattttgc aggatgccgt
    11341 agcggttgcg aatagggcga ttacccagca gaaaaccgca cagatacacc gccagaatac
    11401 cgctaccttc cagcgcagta gttaatgaga aaatcagaat accgccactt aatgccagca
    11461 atggatataa tccggcgggc agggcgatgc ggttgatcat ttgcagtagt aaataaccgc
    11521 cgccaagacc tattacaatg cccagaccaa attgttgcag aatatcgaca atgaacatcc
    11581 agctaatatt gctctcatga tgctggatca tcgcaatcag ggtaatcgtc agaaagaccg
    11641 ccattggatc attactgccg gattcaattt ccagcgtcga gccaacacgt tcgttaagcc
    11701 ccttaccacc cagcagagaa aagaccgctg cagcatcggt agagccgacg atagcgccga
    11761 ttaataagcc ttcaatcaaa tcaagattaa acagccacgc cgccatcatg ccggttaaac
    11821 cagaggtgat aagcacgccc agcgtcgcca gcgacagtgc cggtcctaac gccacacgaa
    11881 aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat aatcgccagt gccaggttac
    11941 ttaccatgta ggcgaagggg taattatcaa acgggatacc gccgacgcca tcgactcctg
    12001 ccagcatgcc gatcgccaga aaaataacca gaataggaat gccaagacgg gaagaaaatg
    12061 aactaagtaa tatactgctg gtgacaagga tggatcctaa gatgaaaagg ctaattattg
    12121 ttgtggcatc caacggtcgg ttactcctga ttacgctgtc tcttatataa accctaccat
    12181 attagcggca gagacagcgt tttacttagc cctgaagcgt gattttttta cattttaaga
    12241 acaggatgac cgctaatcgt caactgagtg ccttcccggg tgttattcag aatggcatgt
    12301 gcgcccaacg gcagcgtaac cgtgcgttgt tcatgaccaa aatcgaggcc ggtaatgagt
    12361 ggaatcgaca ggcgggaacg caaaaacgcg tacactgact ccaggttgta acctgcgtca
    12421 taatcattgg gcgtgctgcc gctaaagcta ccgagaataa tcgccttctg acgtggcaaa
    12481 attcccgcat gatagagctg caacagcata cgttcgaccc ggaaagggtg ctcgttaata
    12541 tcttccagca ccagaatacc gttctcaatt tttggcatcc acggtgtacc gatcagtgaa
    12601 atcagcatcg caagattgcc tccccacaac gtgccttcgg cccgacatgt cgggccttcc
    12661 ccttgccatt caatggtgaa ggtttcattg cgtaacgcca gccagaaatg gtgctcggta
    12721 aaggcgttca gttcatccgc gccaaagttt gctaccagca tggggccgct aaaggtgatg
    12781 acattgccat gcgccagaag accgcactga atggcggtaa aatcgctatg tccgcaaatg
    12841 agcaacgggt catgttgttg gcgagccacc agtgcctgcc agtcaatatc tgccagtaaa
    12901 cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg tgttgggggt agtcagtctg
    12961 gcaagggaat tgagatcttc cagacgctct gtttccgtac ccgcaaaacg ctcacaacga
    13021 cgggcaataa cctcgacgtt attgacttga tgccccgcgt cggtaaggcg ctggatacca
    13081 cgcagcgcgg cgtgctgttt aatgcagtaa cccgatgggg caattaagtg aaacagagac
    13141 atggcaattc cttgctgaca acagaaacga aatgtatatc atgccgctta ggtgtgccgt
    13201 tgtcacctca acggcgattc caggctataa ggatagaaga agtgaaattg agatggtttg
    13261 cctttttgat tgtgttatta gcgggttgtt catcaaagca tgactatacg aacccgccgt
    13321 ggaacgcgaa agttccggtg caacgtgcga tgcagtggat gccaataagc cagaaagccg
    13381 gtgcagcctg gggcgtcgat ccacaattga tcacggcgat tatcgctatc gaatcgggtg
    13441 gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt gatgcagtta aaagcttcaa
    13501 cctccggacg tgatgtttat cgccgtatgg gctggagtgg tgagccgacg accagcgagc
    13561 tgaaaaatcc agagcgtaat atttcaatgg gggcggctta cctgaatatt ctggaaaccg
    13621 gcccgctggc aggcattgaa gatccgaagg tactgcaata tgcgctggtg gtgtcatacg
    13681 ctaacggggc aggtgcgctg ctacggactt tctcgtcaga tcggaaaaag gcgatcagca
    13741 aaatcaacga tttggatgct gacgagttcc tcgaacacgt agcgcgaaat caccctgcgc
    13801 cgcaggctcc gcgctatatc tacaaacttg agcaggcact ggacgcgatg taaatcagtc
    13861 gcgcactttg tccgcttttt cccgggcttc tcgctcgaga gagaaaataa tccgctgtaa
    13921 ttgccgctcc accgtcgggc tgacgttaag aaaacggaag ctcagacggg gagtggtgat
    13981 ggtttcattc ttgccatcaa tcactttgcg ctcgctgatg gagattaact gggcgtcaaa
    14041 gtgaaaaaca ccccattgcc tcatgttgac ttcaatctga gcgaagcgca tgccttcttg
    14101 taattcggca ggctttgctg tttccagtaa tgcgcccatg ccgcctaacg acaaatcata
    14161 caggcggaaa cgtaacgtac tgttatccgc cagtttggtc tggcaaaaat aaggcggatg
    14221 gagtggggcg gagatgcgga aatatcggcg tcgttgtaca aaccataagg tgggaggcgg
    14281 tacggtgata aatgccggaa gctgcaagta ttcactctgc tgtagttgtt caacagtaaa
    14341 ctcgactttc gcaccctgag tttcggcggt aatggtaatg tgctgtgcct ttagcacggc
    14401 gatgttgtct tcggcttgac tgccgaaatc cagcaccagt ttatccgggg ttattgccag
    14461 tattttgctg atcagctgcc cgccattcca actgagacgc aaaggaattg cggctttgtg
    14521 caaatcgcgt aacacgccca ggacggctaa cggattttgt ttcaggaact gctcatggta
    14581 atgactcacg cggaaaaact cctgactgat aaactgtctt tggtttatcg gtcacagtta
    14641 acaaaactta atacaaatgc gtgaatattt tttacatgtt gttcttaaat cagccgacat
    14701 acgcccagca tcgatcccgc ccttgcctat acttagagcg ttgacgtaag catttcttgc
    14761 gtccgattca tcgaaacgag ggcctgaaca tgggaattat tgcctggatt atttttggcc
    14821 tgatagccgg cattatcgcc aagttaatca tgccggggcg tgatggtggt ggatttttcc
    14881 tgacctgtat tctcgggata gtcggtgcgg tggtcggcgg ctggctggcg accatgtttg
    14941 gcattggcgg ctccatcagt ggttttaatc tgcacagctt cctggtggcg gtggtgggag
    15001 ctattctcgt tctgggcata ttccgcctcc tgcgaagaga ataagatttt cataaggcgg
    15061 atagcgatac agatgccgct atccgctttc acatcagaac gtatactcga cacctgcttt
    15121 acgggtgaaa aaaatcaata tagcacttcg atatccgata aagtcggttc ccggtaacaa
    15181 caacagaatc cttttttaat tattgtttcg ttgtttttgg tgtgattcag aaaataaata
    15241 ttttagacag tagccagtca ggcgttaaaa atggggcgga tagtgaggcg gttttggctg
    15301 cgcttattga acatttagaa ttgatcaatc cttctggcag attaagccaa aatacccgtt
    15361 cggcaatgct tcagttacgc gaagaggaat ggtctgaatt ctttttttgg ttgctaaatt
    15421 ctctggagtg tctggattat gtgattatta atttaactcc agaaagcaaa aaaacgttga
    15481 tgagtgaaca ccgtaacaat attcaggtag caattgatgc tttatatagc caacgtcggc
    15541 gtaaatcgcc gggtgatgaa agtgaaacac ttacccgtag aaatgacgct atcttcggca
    15601 accacgtatg gcaaacattt gcgcaatact tcccgccagg acttgaaaaa ccgtctgtgt
    15661 agaaaaaacc atagctatgg tgttatctgc cgacaacgcc atccggagaa gccgaatgac
    15721 gttgtcggag taagcgctgg ttaaggtgtg ggttgtgcct ctttggttga gggttgcgtc
    15781 gttgctgact taacggtcgg acgcgtcgcc ggaacattgt cacacggttg ctctttcggg
    15841 cagatcaaat ccagcatttt cagcgtcacg ccattggtcc agccaaagcc atcctgtaat
    15901 ggatattcgc caccgccgcc ccccgttccg gtggtgctga catcatattt ttccaccagc
    15961 tttttctccc ggtcataggt gtgctgaaca ttggtcagga agtgccagct aatgtccatc
    16021 gccacctctt tttgcccgta gttttgtaat ccttctgtcg cgacccactg taacggtgcc
    16081 cagccatttg gcgcatccca ttgttgccca cttttcaccg acgtggtgtt caggccgccg
    16141 ggttgcagca gatgtgtttt cgtcgccgtc gccattttgt tggcgcgatc tttcgctgcc
    16201 gcattgacgt acagcgggaa cagggcggcc gcggttaact gattgcgcac tttatgactt
    16261 ttcaggtcgt aatcggcata ccagccttgt tgatcgttcc acaggtattt ttcgatccct
    16321 ttttgacggg catttgccag cgtttcgtac tggtttgcca tcgcgttatc tccggcagct
    16381 ttgctggcgc gggcgaggat tttttccatt ttaaacatca ggctgttcag atcgaccggt
    16441 acgatgccgg tggtgcgtaa ggtatttaac tgctgcgggt tgtccatcca gcgcgagctg
    16501 aaatcccagc cagacgcagc ggcagagcgc aggtcgcggt aaatttcagt ggcaggtcga
    16561 ttcggattgc ttttggcggt ggcaatatct tccacccatg actctggtcg tggcgtatcg
    16621 cgatcgtccc agtagcggtt gagaagggta ccatcctgaa gttttacaac gcgtttttcc
    16681 tgttgtccgg cttgcaggtt ttcaacaccg tccatccagt aagcatattc tttttgcatt
    16741 tgcggcaggt attgcttcaa cgcggcatcg ccttcatgct gcgccagtaa ctctaccatc
    16801 agggcaaaga agggcggttg cgagcggctt aaatagtaac tgcggttgcc gttgggaata
    16861 tgaccgtaag tgtctatttc atgagcaaaa ttggccacca tatccgcgac tttatcccag
    16921 tgaccgcttt cggcaagtcc taacatggtg aagtaactgt cccagtaata tacctcgcga
    16981 aaacgtccgc ccggcacgac ataaggttcc ggcagcggta acagagaatc ccatttttcg
    17041 gtgttttcgg tagaacgcgt taataccggc caaagtccgt caatatgttc gcgcagtgac
    17101 tgtccctctg gcggaacata tttctcgccc tctttcggca gggtgaaatt gacgttaacg
    17161 aaatggcgca gatcaaatcc gctctggttt tgctgcatcc gataatcagc aaggatcatc
    17221 agcggatcgc tgttcggcac ggcatcggca aaggtttttt ggtccggaaa aagtttggcg
    17281 ttttgcacat cattaaacag cggccctaat aaaatatcag gcggctgtgg tgttaccggt
    17341 gtttcttctg cctgcaccga tagcgcagcg aaacacaaaa agatgcaggc tggaattaac
    17401 gccatttttt gcgggcgaga aggtgcgggg gatttcatca atcattctcc tttggcgaaa
    17461 ccgaataaaa cggtattcaa gtatcagaaa accttagttc aggatcgcac tagctgcatg
    17521 atcgaaatct cattctgcga actgtcaggc attttagaaa agggaattaa agggataaat
    17581 aacggcgaaa tagagtgatc aaataacgtc tgtttattag ccagccagcg gcgttgggtt
    17641 cgtgattagc gaagaaaaat catcataaat tggtgaaaaa tataacaggt atttaaaaat
    17701 cattagtgct gggtaaattg ccggatgaca tcagaacgat gccatccgaa cggtggctta
    17761 accctgacgg ttgaaacgtt gcgttttaac gtccagcgtt agcgtttctt ctggttgtat
    17821 cgcatacagt ttctcaccct gctggcaaat ccagccaatc cccagttcac gggcgattag
    17881 ggcgctgtgg gataccggac ttccggcgct aaggcagata ccttttacaa ccgccggatc
    17941 cagttgcagt actgtggaag gataaatgtt ctccgccagt agaatagttg gcgagttaaa
    18001 ctgcgggagt tcttctttcg tttgggtcag gtggaccagg gtgcgatgca gaagatcgtc
    18061 cacatcaata tagcgagctt gtagatattc atcatccagt tgctggtatt gctggctaag
    18121 ttctttaaga acttgctgcc aggcatattc tgccgtgcaa tgttcatgct gaaggagttc
    18181 gcttgccgcc gccagcagtt ccggatcatc taacagtgta tggtgaccag aaaagattgc
    18241 ggcaatatcg tcaagcccgc tggcttctgc tttcgctgtt aacgtcatca gatctaataa
    18301 cgtgaagtca atagcctggc gtaatcgttc ttgttcttct tccacggtca gggttgattt
    18361 tgcctgtacc gtacataaaa ctggttgata ataaaaggct ttacccgaaa caggcggaac
    18421 gggacgcaga gtaggtggag cgacttcctc cgtttcacca aagttatctt cagccagctg
    18481 acggaaagcg atcagtgcct cttcagcttc tggccctttc gcaatcaggc gcagcgtatc
    18541 gttatagcga acttgtagta acgcaatctg gttaatactc tctggtgtga cgcatttgcc
    18601 gtttttttcc agcaacatat cggcattaaa tgtcgataag gtataaacca gccgggaggc
    18661 cggacgtaca tgcaggccgt tacggttttt tatgaccacc gccagagaac gggcttcttc
    18721 atcgtacgca ggacatgtgt cagagatttc agtgtcggag gacggtaaac ccagttgttc
    18781 acgtttggct tccagcgcat gcatagcgtc aaagataact ttgtcgatat ccgctcccga
    18841 ggccgcgctg accgttgctg ccagtgtacc ttcgaccaac ggcgcagcac acaaacgtac
    18901 ttttgtggcg atctcggtag ccagcaattc cagcgcagtt tcagcactca ataatgcgct
    18961 acccatatcc atcatgacca gcacatggtc ggcatcagca acagattcga tggcctccat
    19021 cactttgacg gcatcggtac caatgggatt ttgtggatcg tcaattcccg cggcaatggc
    19081 gattttacaa ctatcactca ttaacatctg acgggctaat tcaccgacac cttctcccag
    19141 tcggctgcta tgtgaaacta tgaccaggtt taccatcacc aattccttac tcttttgcgg
    19201 ctaacgccaa catttgcatc ataaacatca ccgaggtcgc gccgggatcc tggtgaccaa
    19261 tactgcgttc accgagataa ctggcgcggc ctttgcgggc ttgcatcgta atcgtacttt
    19321 gtgcagcgga ttcggcgatg ctactggcag cttcgagcgc cgccggaaca gagagatttt
    19381 gctcgctgga ctgacgtaac gattccacca ccggcaccca cacatcacac atggttttat
    19441 cgccaggttc ggctttcccg cgactgatta cgccgtccgc gccatcgcgg aacatctgat
    19501 aaagctcttc cagcgtcagg ctttgccgtg cctgggtcgc ctgtgcggcg cggataaaga
    19561 aggtaccgaa cagcggacca ctggcaccac cgacgctgga aagcagcgtc ataccggtat
    19621 tcttgagaat gaaaccgata tctttatctg cgatagcagg gagtttttcc actactttgc
    19681 taaagcctcg gttcatattt agcccgtggt cagcatcgcc aatttcgcga tccagtccgg
    19741 taagatactc gctctcggtg ctgaaaatat cgccacaacg agtgagccag ttaacaattt
    19801 gagttctgct cagtgacatt gctttctcct tatttacccc agttaagggc cggggtgtgg
    19861 accggggcgt cccagagtgc cagcgtttcg tcatcaactt tcagtaaggt gattgagaaa
    19921 ccggtcatat ccagtgaggt gcagtacgcg ccaattaaat tacgttcgat agtcaatccc
    19981 gcttgctggc aacgtgtggt caggcggtta tagacgccgt acagctcaga aagcggagtt
    20041 gcgccaagat tgttaaccag cgcaatcacc cgatcgccag actggagcgg ttgtttggtt
    20101 tgttgttctt cctgccaact gccttgttga taatcccaga aacgcaaagt gcgatggtat
    20161 gagccattta ccagcagggt gtcgaacatt tcatcgacgg tttgatcaag ggaagagaag
    20221 gggcggcggt caatacccgg ctcaccatga atgccgacgc caaactccat ctcattatcc
    20281 gccagggtaa aagaaggttt gcccgcggca ggaacggtac aggcaccgag agcgatacca
    20341 attgagtggc cttgattatt caacttacgc cccagttccg cacaggcgtc cagtgagtcg
    20401 ccacgctccg ccgcagcgcc tacgagtttt tcaattaata cggtgttggc aacgccgcgc
    20461 cgcccggcgg tataaagact gtcttttact gcaacgtcgt catcaatgac cacagtggtc
    20521 acttttacgc cgctatcgtg cagtaactcg gtcgctgttt caaagttaag aatatcgccg
    20581 gtgtaatttt tgataatcaa cagtacacct tcgccgccat caacttgcat ggcgcattca
    20641 aagattttat cgggcgtcgg tgaggtgaaa atttcgcccg gacaggcccc cgaaagcatc
    20701 ccctgaccga tataaccaca gtgcatcggc tcgtgtccgc taccgccacc cgacagcagg
    20761 gcgacttttc ctgtaacggg ggcatcagct cgggtgacat acaccggatc ctgatgcagt
    20821 gtcagcgatg gatgcgcttt cgccagtcct gccagttgtt cgtccagtac gtcttgcaca
    20881 tcattgatca attttttcat tattttgctc cagcaattac ggtagggcat ggatgatgtt
    20941 caacgacacg gcgaccactg actgccgatg aatccattgt gcatcaggca agggaaaaga
    21001 aaattagccc aaattatgtt tcatagtgaa acatatgctt taatgaatgt tccatattga
    21061 aacttttacg tgtattaata cttaaaattg cgagccggaa caccttttgt cataagggat
    21121 gcgggatatg agtggcgctt ttaacaacga tggtcggggc atatctccct taattgcaac
    21181 ctcctgggag cgatgcaata agctgatgaa acgggagaca tggaacgtac cacatcaggc
    21241 ccagggcgtg acatttgctt ctatttatcg gcgtaagaaa gcgatgctga cgctcgggca
    21301 ggctgcgctg gaagatgcct gggaatatat ggcaccgcga gagtgtgcgc tgtttatcct
    21361 cgatgaaacc gcctgcattc tcagccgtaa tggcgatccg caaaccttgc agcagctaag
    21421 tgcactggga ttcaatgacg gcacgtattg cgccgaggga attattggta cttgtgcgct
    21481 atcgttagcg gctatctctg gtcaggccgt gaaaacgatg gccgatcaac atttcaaaca
    21541 ggcactctgg aactgggcct tttgtgcaac gccgttgttt gacagcaagg gccgattgac
    21601 gggaacaata gcgctggcgt gtccggttga gcaaactacc gcagctgatt tgccgttgac
    21661 gttggcaatc gcccgcgagg tcggaaattt actgctgacg gacagtttgc tcgctgaaac
    21721 taaccgtcat ttaaatcaac ttaatgccct gttagaaagt atggatgatg gcgtgattag
    21781 ctgggatgag cagggtaatt tgcaatttat taatgcccag gcggcgcggg tcttgcgcct
    21841 tgacgcgacg gcaagtcagg gacgggcaat cactgaactc ttaacgttac ccgccgtatt
    21901 gcaacaagca ataaaacagg cacatccgct caaacacgta gaagcaacct ttgaaagcca
    21961 gcaccagttt attgatgcgg tgataaccct taaaccgata atagaaacgc agggaaccag
    22021 ctttattttg ttgctccatc ctgtggaaca gatgcggcag ttaatgacca gtcaattagg
    22081 aaaagtcagc cataccttcg ctcatatgcc acaggacgat ccgcaaaccc gccgcttgat
    22141 tcattttggt cgccaggcgg cgcgcagtag ctttcctgtc ctgctttgtg gagaagaggg
    22201 cgtgggcaag gcactgctaa gtcaggcaat tcataatgaa agcgagcgtg ctgcaggtcc
    22261 ttatatcgcc gtcaattgtg agttatatgg tgatgctgcg ctggcggaag aatttattgg
    22321 tggcgatcgc acggacaatg aaaatggccg tctgagtcgg ctggaactgg cacacggcgg
    22381 cacgctgttt cttgaaaaga ttgaatatct ggcggtggag ttacagtctg ctttgcttca
    22441 ggttatcaag cagggggtta tcacgcgact ggatgcgcgg cgtttaatac caattgatgt
    22501 caaagtgatt gccacaacga ccgcggacct cgcaatgctg gtggaacaaa atcgttttag
    22561 tcgccagctg tattacgcgc tgcatgcatt tgaaattacc atcccgcctc tgcgtatgcg
    22621 gcgtggcagc attccggcgc tagtgaataa caaattacgc agtcttgaaa aacgcttctc
    22681 tacgcggctg aaaattgatg acgatgccct cgctcgcctg gtttcttgtg catggccagg
    22741 caacgatttt gaactttaca gcgtcatcga gaatcttgct ctgagtagtg ataacgggcg
    22801 cattcgcgtc agtgatttgc cggaacatct gtttaccgag caggcgacag atgatgtcag
    22861 cgccacccgc ctttccacca gtctgtcatt tgcggaagtt gaaaaagagg caattattaa
    22921 cgcagcccag gtcacaggcg gtcgcattca ggaaatgtcg gctttacttg ggatcggccg
    22981 cactacgctg tggcggaaaa tgaagcaaca tggcattgat gcagggcagt ttaagcgccg
    23041 ggtatgaaag acagaaacga tttctgatac atcagagtga tctgtatttc attccggcgc
    23101 acgctaacaa ttttcagcat cgtttaaggg cttgtctatc ccgcacttaa aagctgaagc
    23161 gatatcctcc gttgacttgc ttctgatcaa agaggttacc ctgcgtgtaa tccgcttcga
    23221 gatagaatgt gtgctgtttg ttatactgtg cactgactcc cacgccgtta ttccagccat
    23281 tacctttgaa actgtacttc tcccgggagt tgttcaacag atattcggta tcccctgaaa
    23341 actcgcggat agcgccagtc ttcacataga cattcagctg gctgttgcct gcggtgatgt
    23401 cataccccag tatcatgctg gcacgcccca gcagcgattc gtagtgattc agatgtatat
    23461 tgaggccatt actcgccttc atagtcatct cattctggtg gctgtatgta agctgggttt
    23521 gcggctctat atagaaccca taaccagtag gggacaggtt gaacctctgc ccggcttcca
    23581 gggagatgct cattccattc gcagtgccgt tggcgttaac gccgttgttc tgactgtcca
    23641 gtacgtggaa actatttttc tggcgcgatg cttttataac gagatcgctg taaaaaccgt
    23701 tttgtgccat gtaactggcg tacattccca tgtagtctga acgtgcggta ccgtcgcctc
    23761 cgctatagtc cggcgatgca tgtgttgagc caatatacag accgacatac aacggcatta
    23821 catcagagag acgtttatcc ccaccaaact ggataccgct gtaacccatg tcaaagccgc
    23881 tcagtttgcc actggcaaag gagtccaggc ttcccccata actgcgcaac cagatattac
    23941 cgtctttact ctgatttcgc aggtcaccca tccgttgcat cagcgtacgg ttttcaacat
    24001 agttcaataa gtaaccgaca ttgagataat tgccgccagc atctgcggtc gtggtgggtt
    24061 taggcgtggg agcgggttca ggcgtaggat cggggttgac tatgggaggc tgagcgggag
    24121 ctggtgtggg ttcaggatta ggagtcggtt ccggaactgt cccggaagcg taaagctccc
    24181 agttagtgcc atttttacgc acatcgtaca gatatccccc caactcaacc tgagaagacg
    24241 cgctgaacga ggccgcgccg tcagtggttt ttaccactgt cagaacttca tttcccgttg
    24301 tggcctcgct gccctggttg cggatagcca atacgtgatt accagcacta ctcccgctga
    24361 tattcaataa atccccttta ttattaacgc cattaccctc gccaacaaca tcagcacgca
    24421 taataaaggt actgttaccg ctcaggttct ctacgtttaa tgtggcaaat gtgccggcag
    24481 ttgacccgtg gctggcaaaa tcgacagttg aatggctcag cgccagcgtg tcgagattag
    24541 aattacttgt tacgttccag acgctgttat tcattgcaac gtccagtttt ccgccattga
    24601 cattatcgct gagggaggaa cctgtccaaa ccgacccagg gtgcatatcc agattgatta
    24661 gcccaccttt ggaaagaacg ctaccgttga taagcatacg acccgaggcg ttgatgcggc
    24721 tggcggcata accatcgtca tgttgcgttg cgatggccat ctggtcgggt gtactcatat
    24781 caatgaccag atcgctcgtg aggtcgatct ggctgttggt catggcataa attcccctgg
    24841 ctcctgccgc gccggtgata gccaaactgt ctccggttat tctaccgccg ctgagagccc
    24901 acaaacccag cgccagactg ccattacgat caactgtaat atcggtattt tgcatgttga
    24961 caacagccgt tgccgtctgg gccgaggcac cataagaacc gccggaaaag atgctgtttc
    25021 gttgcgctgc cgtgccagta aaattgatta tcgcgtctga accactggtg acgaggccac
    25081 cgccctgcgc ggaagaaata tggctatctg caccgatagt ggttgtacca ccacgaactt
    25141 cgacgccatt ggccgcggct ccagttacat caacagtgag tgcattcgcg ctaacctggc
    25201 caaagctcca gaggccgtga gcattatcgc cattggtttt aatggtactg tttgttccga
    25261 gatcgacaac agagtttttc tgtacgttta tccccatggc gctgtagccc tgaacatcga
    25321 ttgtcaggtc tgtagccgta aaacgcgcag caccattggc gttattgccg ttgagaccac
    25381 cgatataaac acctgtactt ccatcggtcg tgattttact tccgcttcca agatcgacac
    25441 tggtgccata gtcattgatg gttaggccta taccgttcga gttttcaatg gtgaattgag
    25501 tggctgttag tgttgagcta tgcccaataa tgatgccgtc atcgttactc ttaatggtgc
    25561 tgcctgtgcc taagtcagca tgggtatagt caccaattaa gttaatgccg atggcagagg
    25621 tttgaccaac aacatctact gtgagtcggt tagctgtcag ggagctgttt ttgccctgaa
    25681 taatgattcc ttttgcatat ccagaggcgt cgttgacatt gacggtgaca tcattaccca
    25741 ggttgatatt gccaggcgtg tttcctgctg gggtcattac actatagagg tttcctgatg
    25801 gatcggcggt atctcctgta attctgtcac catcattaat agtaatattg gtgccatatt
    25861 gacttcctgt atcagcagct atcagcaggg gggaaaatat aattgcattc aaaccaatag
    25921 cgccaaattt tatcagttga attgagggcg aacggatttt taattcagcg agacgatctg
    25981 ctttggtatt cccattgtgt tgtttgatgc ccataccact cctatatagt acccagacta
    26041 tgggtctatg gattttgtca ttttgtttaa atcaatgaat aatatcctct tatcatcatg
    26101 atatttgtat ggtgcaatca tgatgagctt ctcagagaat aattctctat taccgggcaa
    26161 aatcaggaac acaggaatta atatatattc aacatccccc ttacaaaggt gagtttatat
    26221 cgagttattc cctaagttgt cgttacttat tccaattcgt tactataaat atggcaaaaa
    26281 tattacaaca aacagcggtt aaaaaatttg gtgaagattt tttgtaataa tcctaattag
    26341 ttatgtgaat ttcgcttgat gtatctgcat aacaaaaaca atatgcgcca tttttgcatt
    26401 actaataaga aaaagcaaaa aacatcgata ttcatttttg gtcaatgggt tttctggcct
    26461 atcgttgtta ttgtgctact ggttatcatt ttcatcttgc cagcatattg gagcgtgatc
    26521 aattttgatc agctgtgaag ggggctgatg tgtagtgata caaaaactta cgttatatca
    26581 ctgctaatga gatatccgtc atctgaacac ttatgtcttt aattattctc gtggttcact
    26641 ataggcaata agcacaaaag tgtaggatgt tacaagaatg attaggactc ggtgaaatga
    26701 aaaatccacg caattgcgtg gattttatat acttttgcgc tcttcatgag atttagcgaa
    26761 acctcatgag acaataaatt aattagacgt tgaaaaggaa gttcatcaca tcgccatctt
    26821 tcacgatgta atctttacct tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac
    26881 ctttgtaagt gatgaaatct tcaaacgaga tggtttgtgc acggataaag cctttttcaa
    26941 aatcagtatg gattttgccc gctgcctgcg gcgcggttgc tccaaccgga atggtccatg
    27001 cacgcacttc tttcacccca gcggtgaagt aagtttgcag gttcagcagt ttataaccgg
    27061 cacggatcac acggttcagg cccggctctt ccagcccaag ctcctgcata aactcgtcac
    27121 gttcttcgtc gtccagttcg gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa
    27181 cagaaccttc tttcgccgcg atttcacgca cctggtcaag atatgggttg ttttcaaaac
    27241 cgtcttcgtt gacgttggcg atgtacattg ttggtttcag cgtcaggaag ctcaggtaac
    27301 gaatagccgc tttctcttca gcgcttaaat ccagcgcgcg cagcatacct gcattttcca
    27361 actggggcag gcatttttcc aggaccgcca gctcagcttt cgcgtcttta tcgccacctt
    27421 tggctttctt ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca
    27481 gttcggtgtt gataacttca atatcgtcag ccgggttaac tttgccggaa acgtgaatga
    27541 tgttgtcatt ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg
    27601 tcaggaactg gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat
    27661 cgacaaattc catggtcgtg ggaagcgtac gctgcggttt tacgatttca gccagttgat
    27721 ccaggcgagg atcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa
    27781 agttggccgc ttcaataccg gctttggtca gcgcgttgaa cagggtagat ttcccgacgt
    27841 tgggcaaacc gacgataccg catttgaatc ccatgattta aatcacctta atatcttaat
    27901 aatcaacctg ttattgctaa cagattgcag aaatggaaat aactttgcct attatacacg
    27961 gcactcggca aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt
    28021 cgttgctttg gtcaagccat ctgtaaacca catttcagta caacgcgccg cttcgtcaat
    28081 ggcttcatca attaacttct gttcactaac aggcggtttg cctaacacaa aaccgacaac
    28141 tttattttta tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt
    28201 acccaattta ctgatgatgt ctttcagtcc attgtgacca ccatggccac cgcccaattt
    28261 aaatttggcg acgccaggag gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg
    28321 attaatgcgg aaaaaactgg ccatcgccgc aacggctttg ccgctgagat tcataaatgt
    28381 agtcgggact aacaggcgga catcttcgcc tccaagagtg actcgcgaag tataaccaaa
    28441 gaatttagcc tcttcgcgca gcggagcgcg caaacgctct gccagtaagt caacgaacca
    28501 ggcaccagca ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat
    28561 caatttaatc gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg tagtttactg
    28621 gttgcggccc tgcttgacaa aaaactgcat atcaaatgca gataacgtaa taattgcctg
    28681 agtggactat tagaaagtca aggtgttcag gcgtttattt gtaaagtttt gttgaaataa
    28741 gggttgtaat tgtgatcacg cccgcacata acccactggg tgttgtctat actttacaca
    28801 taaggaagag gggtattccc tgttacaacc cagaaagttc cggaggtgac atatgaaacg
    28861 caaaaacgct tcgttactcg gtaacgtgct catggggttg ggtctggtgg taatggtggt
    28921 cggcgtgggg tattcaatcc tcaaccagtt accacagttt aatatgcccc agtatttcgc
    28981 acatggtgca gtgctaagta ttttcgtcgg tgccattctc tggctggcgg gtgcccgtgt
    29041 tggcgggcat gaacaggtgt gcgaccgtta ctggtgggtt cgtcactatg acaaacgttg
    29101 ccgccgtagc gataatcgcc gtcatagcta acaaataatg cagtttgctg accagtcagt
    29161 ttgcgctgac tggtcaatct cgtacttata aatccgccat cgccgcgcga cgattcggga
    29221 agaacgcgag gcgtcccggg atcggttgaa tgcctgcgcg agccatagtg cgcagtggct
    29281 ggaattccac gttgcacacg cgcagttcac atccttcggg cagacgcttc acaaaacgct
    29341 ggaacgcatc aagaccacca gcatcaagta ccggaacggc atcccacttc agaatcacaa
    29401 tccgtttgcc ttcaagacgt gactccaggt ccgtgaacaa gccttcagca gcagcaaaaa
    29461 acagcgggcc aataacgcgc agtaccagaa catcgtctgg aacatctacg actaccggtg
    29521 ccaggcgagt catacgtgcg atacgacgca taaacagcag cgatgccagc acgatcccca
    29581 cgctgatggc aataaccata tcaaacagca cggtcagcga catgcacagc agcatgacaa
    29641 tgatgtcatc tttcggtgca tgacgcagca agtcgaccac tttatgcgct tcactcatgt
    29701 tccacgccac catcaacagc agggctgcca tagcggaaag cggcagccag gagagcagcg
    29761 gtgccagtac cagcagggca agaataacca gaatagagtg gatcaccgcc gagatagggg
    29821 aagttgcccc ggcacggacg ttagcggcag aacgcgcgat ggcagctgta gcggtaatac
    29881 caccaaagaa cggagcgata atattcccca gtccctgtcc aaccagttcg ctgttcgcct
    29941 tgtgtttcgt cccggtcata ccatccagta ccacggcgca gagcagagat tcgattgcgc
    30001 cgagcattgc cattgagaat gccgcaggca gcagtgtgcg aatagaatcc caggttagcg
    30061 tgaattctga attaggcaga tcccacggca gcaccagttg cggcagcagt tgcggaatac
    30121 cgttaccctg agaaccatcg gccaggacgt agtggaattg cgaaccgatg gtagcaacat
    30181 gtccgccgag caggttaaca atccccatca ccgcgcaacc agccagcaag gccggaaggt
    30241 gaccgggtaa acgaatgccc agacgcggcc aaaaaacaag aatacctagc gtcacaatgc
    30301 caatggcagc atcacccaca ttaatggtcg gcagcgccat aaataatgcg ccgacttttt
    30361 gtagataatg ttccgggaca tgggccattt gcagaccgag aaaatcttta atctgcatgg
    30421 taccgatggt gatcccgata cccgaggtga aacctaaggt gacggaaacc ggaatatact
    30481 caatcaggcg accaaagcgt gccagaccca tcagaatcaa aaagatcccc gacagcaagg
    30541 tcgcaaccag cagtcctgcc agtccaaact gttgcgacac gggatagaga attaccacaa
    30601 atgccgcagt cggaccggaa acgctaaagc gtgacccacc cgtcagagca atgacaatcc
    30661 ccgcaacagc tgcggtatat aaaccgtact ggggtgccac accactacca atagccaacg
    30721 ccatcgccag cgggatagca ataatcccga cggttatccc ggcaatcagg tcacgggtaa
    30781 accgtgcggc agtatatttt tctttccagc aagcgtcgat cagagcgcgg aaaggcatca
    30841 catgtgagga aaatattttg ttcacaataa tgtttcatcc gtgagcgcat catctgtcaa
    30901 ctaaatggca ggtgaaggag gcataggtca tacaaatgga tattacagac aaaaaaaccc
    30961 gccgcagcgg gtctttgagc cgggttcgat tagtgttcga acatggcaga gatcgattct
    31021 tcgttgctga tacgacgaat cgcttcggcc agcatacctg acagggtcag agtacgcacg
    31081 ttcggcagtg atttgatttc atcgctcagc ggaatggtat cgcagacaac gacttcatca
    31141 attacagagt tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg agtcgcgtac
    31201 gcaaatacac gtttagcacc acgttctttc agagcttcag cagctttaca cagcgtaccg
    31261 ccagtgtcga tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc accgatgata
    31321 tgcatcacct gtgaaacgtt cgcacgcgga cgacgtttgt cgatgattgc catatcggta
    31381 tcgttcagca gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc
    31441 acaattgggt tatccagatt cagctgcagc atgtcttcca gcaggatcgg gctaccaaat
    31501 acgttatcaa ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc
    31561 agcacacggt caacaccgac gctggagagg aagtctgcaa ccactttcgc agtgattggt
    31621 acacgagcgg aacggacgcg acggtcctgg cgcgcatagc caaagtaggg gataacagcg
    31681 gtgatacgac ctgcggaagc acgacgcagg gcatcaacca taacgactaa ttccatcagg
    31741 ttgtcgttag taggggcaca agtggactgg atgatgaaaa tatcaccacc gcgtacattt
    31801 tcattaattt gtacgctgac ttcgccatcg ctaaagcgac ctacagcggc gtcgccgagt
    31861 gaagtgtaca ggcggttggc aatacgttgt gctagttccg gggtggcgtt accagcaaaa
    31921 agcttcatat caggcacgag aagaacctca ggcatgcgtc cattggtgga aagaatctgc
    31981 cgaaaactgt gcgggccagg catgatcctt tccaggcggt gtattaaaga gcgcgatgca
    32041 acgtctggaa caaggtgacg ttgtcaccga aactcagctt gcccggctta aagcatggct
    32101 ctgtgcaatg gggaaagatt aacgcctttc gccacaaagc cattgagcca ttccggggct
    32161 tgctctagca cctggcgggc ttcagactct gtatcaaatt cagcaaagac acaggcccct
    32221 gtcccagtca ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc atcaacctcg
    32281 cgaaaacgtt ttcttgcgat aacctcgcaa tcattgctga attcacattt tagcaacgtt
    32341 tctattgacc tttttggcgt attgcgcggg agttcaggat ctttaaaaat caccggagtc
    32401 ggaatactta caccagggtg cgccaccaga taccacttct ctggcggatc caccggcgtt
    32461 agtatttcac caacgccttc ggcaaacgcg gcatgccccc gaacaaagac aggaacatct
    32521 gcgcccagcg tcagccccat ttccgccagc tcatccatgc ttagcccgca ttgccagaga
    32581 tgatttaatg ccaccaggac cgtcgcggca ttggatgaac caccgccgag accgccgccc
    32641 atcggcaaac gcttatcaat gctgatatcc gcaccgcttc ccgtcggaag acgcccgctg
    32701 tctgccgcag ttttcatcaa caaccgcgct gcgcgaacga tcaggttatc ttcatgttcc
    32761 acgccttcaa cgggcgttaa cagacgaata tccccatcgt cacgaagctc aatgctgatg
    32821 gtgtcgccgt aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc
    32881 tgaccggtaa tgtataaaaa cagattaagt tttgccggag agggccactg tgtccgcatt
    32941 atttcactat ccagttatcc atttttaact tgatgcgttg accaccgtcg gtgagttcca
    33001 tattggctgg catcgcaggt tgcgttttgg tgtcataacc accataaaca accttccagt
    33061 ttttgccatt ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc agtttgtagt
    33121 cggttgcatc acccggtaaa cctaaaatcc actggcgcaa gctgttgagc ggaattggca
    33181 ttccggtcaa tttgccaatc atctcttcgg cgtcatcggc ggtataacgc tgacctttat
    33241 tgtcgactaa ctgcacgtta cccggttgag cattcagctc cagttccgtg ctgcccaatg
    33301 ggttagtgag cagcagacgg tagcgatcct ggccggtttg ctgccagaaa aagcgggcgt
    33361 acactttttg ttggtcagaa atataagcga acgcgccgcg agtctgatac tgattaagat
    33421 tgcgcacgtc ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg ccaggacctt
    33481 tgggcgtggt aacggaacag gcagtgagca caagagcagc cagcggtagc aggcggataa
    33541 gacgaaaatc gggcaggggc atagtgatga caagtccttg agatacgttg cagttataac
    33601 ccttaatgct agcgttaccg tccgctatcg tctatgttca agttgtctta attgccagaa
    33661 tctaacggct ttcggcaatt actccaaaag ggggcgctct cttttattga tcttacgcat
    33721 cctgtatgat gcaagcagac taaccctatc aacgttggta ttatttcccg cagacatgac
    33781 ccttttagca ctcggtatca accataaaac ggcacctgta tcgctgcgag aacgtgtatc
    33841 gttttcgccg gataagctcg atcaggcgct tgacagcctg cttgcgcagc cgatggtgca
    33901 gggcggcgtg gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg ttgaagagca
    33961 ggataacctg caagaggcgt taatccgctg gctttgcgat tatcacaatc ttaatgaaga
    34021 agatctgcgt aaaagcctct actggcatca ggataacgac gcggttagcc atttaatgcg
    34081 tgttgccagc ggcctggatt cattggttct tggggagccg cagatcctcg gtcaggttaa
    34141 aaaagcgttt gccgattcgc aaaaaggcca tatgaaggcc agcgaactgg aacgcatgtt
    34201 ccagaaatct ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg gtgccagcgc
    34261 tgtgtctgtc gcttttgcgg cttgtacgct ggcgcggcag atctttgaat cgctctctac
    34321 ggtcacagtg ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg
    34381 cgaacataaa gtacagaaga tgattatcgc caaccgcact cgcgaacgtg cccaaatact
    34441 ggcagatgaa gttggcgcgg aagtgattgc cctgagtgag atcgacgaac gtctgcgcga
    34501 agccgatatc atcatcagtt ccaccgccag cccgttaccg attatcggga aaggcatggt
    34561 ggagcgcgca ttaaaaagcc gtcgcaacca accaatgctg ttggtggata ttgccgttcc
    34621 gcgcgatgtt gagccggaag ttggcaaact ggcgaatgct tatctttata gcgtggacga
    34681 tctgcaaagc atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga
    34741 aactattgtc gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag
    34801 cgaaaccatt cgcgagtatc gcagccaggc agagcaagtt cgcgatgagt taaccgccaa
    34861 agcgttagcg gcccttgagc agggcggcga cgcgcaagcc attatgcagg atctggcatg
    34921 gaaactgact aaccgcttga tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga
    34981 cggggataac gaacgcctga atattctgcg cgacagcctc gggctggagt agcagtacat
    35041 cattttcttt ttttacaggg tgcatttacg cctatgaagc cttctatcgt tgccaaactg
    35101 gaagccctgc atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc
    35161 gccgaccagg aacgttttcg cgcattatca cgcgaatatg cgcagttaag tgatgtttcg
    35221 cgctgtttta ccgactggca acaggttcag gaagatatcg aaaccgcaca gatgatgctc
    35281 gatgatcctg aaatgcgtga gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc
    35341 gagcaactgg aacagcaatt acaggttctg ttactgccaa aagatcctga tgacgaacgt
    35401 aacgccttcc tcgaagtccg agccggaacc ggcggcgacg aagcggcgct gttcgcgggc
    35461 gatctgttcc gtatgtacag ccgttatgcc gaagcccgcc gctggcgggt agaaatcatg
    35521 agcgccagcg agggtgaaca tggtggttat aaagagatca tcgccaaaat tagcggtgat
    35581 ggtgtgtatg gtcgtctgaa atttgaatcc ggcggtcatc gcgtgcaacg tgttcctgct
    35641 acggaatcgc agggtcgtat tcatacttct gcttgtaccg ttgcggtaat gccagaactg
    35701 cctgacgcag aactgccgga catcaaccca gcagatttac gcattgatac tttccgctcg
    35761 tcaggggcgg gtggtcagca cgttaacacc accgattcgg caattcgtat tactcacttg
    35821 ccgaccggga ttgttgttga atgtcaggac gaacgttcac aacataaaaa caaagctaaa
    35881 gcactttctg ttctcggtgc tcgcatccac gctgctgaaa tggcaaaacg ccaacaggcc
    35941 gaagcgtcta cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg taaccgtact
    36001 tacaacttcc cgcaggggcg cgttaccgat caccgcatca acctgacgct ctaccgcctg
    36061 gatgaagtga tggaaggtaa gctggatatg ctgattgaac cgattatcca ggaacatcag
    36121 gccgaccaac tggcggcgtt gtccgagcag gaataatgga atatcaacac tggttacgtg
    36181 aagcaataag ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa atcctgctgg
    36241 agcatgttac cggcagaggg cgtactttta ttctcgcctt tggtgaaacg cagctgactg
    36301 acgaacaatg tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt gaacccattg
    36361 ctcatttaac cggggtgcga gaattctggt cgttgccgtt atttgtttcg ccagcgacct
    36421 taattccgcg cccggatacg gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac
    36481 aaccttgccg tattctcgat ctcgggacgg gtaccggggc gattgcgctt gcgctggcta
    36541 gcgagcgccc ggactgcgaa attatcgctg tagatcgtat gcctgatgct gtctccctgg
    36601 cacaacgtaa tgcccagcat ctggcgatca aaaatatcca cattctgcaa agcgactggt
    36661 ttagcgcgct agccgggcag cagtttgcga tgattgtcag caatccgccg tatattgacg
    36721 agcaggaccc tcatcttcaa caaggcgatg tccgctttga gccgctcact gcgctggttg
    36781 cggcagacag tggaatggca gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg
    36841 tatccggcgg ctttctgctt ctggaacatg gctggcagca gggcgaagcg gtgcgacaag
    36901 catttatcct cgcggggtat catgacgtcg aaacctgccg tgactatggt gataacgagc
    36961 gcgtaacgct cggccgctat tatcaatgac aagtttttct acactgctta gtgttcatct
    37021 tattagtatc gcgctttctg ttgggctatt aaccttacgt ttctggctac gttatcagaa
    37081 gcatccacag gcatttgctc gctggacgcg cattgtgccg ccggttgtcg atacgctgtt
    37141 actgttaagc ggcattgcgt tgatggctaa agcgcacatc ctgccatttt ccgggcaggc
    37201 acagtggctg actgaaaagc tgtttggagt tatcatttat atcgttttgg gttttattgc
    37261 actcgattat cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc cgctggcgtt
    37321 ggtggtgctg tacatcatca ttaaactcgc caccacaaaa gtaccgttac tggggtaagt
    37381 catgagatcg ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct
    37441 ggcttgcgaa gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg aactggagcg
    37501 tctcgttagt ctggcgaagg aagaaatcag ccagcttctg cctttagaag agcagttgga
    37561 aaaactgatc gcgctgtttt acggcgactg gggatttaaa gcctcacgcg gtgtttatcg
    37621 tctttccgat gcattatggc tggaccaggt gttaaagaat cgacagggca gtgcggtatc
    37681 attaggtgcg gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat
    37741 tttccctacg cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa
    37801 tccttttaac ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat
    37861 cagcccgtcg gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat
    37921 ccgcaaattg ctggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc
    37981 gttacgcacc agcgaagctt tattacaatt caaccctgaa gatccctatg aaattcgcga
    38041 tcgcgggttg atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta
    38101 tttcgttgaa cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa
    38161 catcgcgcat aaacatattg tgctgcatta attaatcgac attttactca agattaaggc
    38221 gatcctatga aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg
    38281 ttcgtactgt ttggcggtat gaacgtgttg gaatctcgcg atctggcgat gcgcatttgc
    38341 gagcactacg taactgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt
    38401 gacaaagcca accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg
    38461 aaaatcttcc aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa
    38521 ccaagtcagg cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt
    38581 gctcgccaga ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag
    38641 aaaccacagt ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc
    38701 ggcaacgaaa aagtgattct ttgcgatcgc ggtgctaact tcggctatga caacctggtt
    38761 gtcgatatgc tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc
    38821 gacgtgaccc acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt
    38881 gctcaggtgg ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt
    38941 gaagcgcatc cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct
    39001 aaactggaac cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa
    39061 gaactggata ccagcaagta atctttttgt ttgaaaaata aagtcttagc gttgtgcgtt
    39121 aagacttttt tatgggtgcc ggatacaaaa a
//
LOCUS       DACAPB010000019        38973 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.019, whole genome shotgun sequence.
ACCESSION   DACAPB010000019 DACAPB010000000
VERSION     DACAPB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.019"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="IT904_000895"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="IT904_000895"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAP3190721.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLHSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(1698..3323)
                     /locus_tag="IT904_000896"
     CDS             complement(1698..3323)
                     /locus_tag="IT904_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAP3190722.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3782..3868)
                     /locus_tag="IT904_000897"
     tRNA            complement(3782..3868)
                     /locus_tag="IT904_000897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Leu,seq:gag)
     gene            complement(3883..4215)
                     /gene="secG"
                     /locus_tag="IT904_000898"
     CDS             complement(3883..4215)
                     /gene="secG"
                     /locus_tag="IT904_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP3190723.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4443..5780)
                     /gene="glmM"
                     /locus_tag="IT904_000899"
     CDS             complement(4443..5780)
                     /gene="glmM"
                     /locus_tag="IT904_000899"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP3190724.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5773..6621)
                     /gene="folP"
                     /locus_tag="IT904_000900"
     CDS             complement(5773..6621)
                     /gene="folP"
                     /locus_tag="IT904_000900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAP3190725.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6711..8645)
                     /gene="ftsH"
                     /locus_tag="IT904_000901"
     CDS             complement(6711..8645)
                     /gene="ftsH"
                     /locus_tag="IT904_000901"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP3190726.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8745..9374)
                     /gene="rlmE"
                     /locus_tag="IT904_000902"
     CDS             complement(8745..9374)
                     /gene="rlmE"
                     /locus_tag="IT904_000902"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAP3190727.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9500..9793
                     /gene="yhbY"
                     /locus_tag="IT904_000903"
     CDS             9500..9793
                     /gene="yhbY"
                     /locus_tag="IT904_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP3190728.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9949..10425)
                     /gene="greA"
                     /locus_tag="IT904_000904"
     CDS             complement(9949..10425)
                     /gene="greA"
                     /locus_tag="IT904_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP3190729.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10673..12106
                     /gene="dacB"
                     /locus_tag="IT904_000905"
     CDS             10673..12106
                     /gene="dacB"
                     /locus_tag="IT904_000905"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP3190730.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12292..13464)
                     /gene="cgtA"
                     /locus_tag="IT904_000906"
     CDS             complement(12292..13464)
                     /gene="cgtA"
                     /locus_tag="IT904_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAP3190731.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13480..14445)
                     /locus_tag="IT904_000907"
     CDS             complement(13480..14445)
                     /locus_tag="IT904_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP3190732.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14572..14829)
                     /gene="rpmA"
                     /locus_tag="IT904_000908"
     CDS             complement(14572..14829)
                     /gene="rpmA"
                     /locus_tag="IT904_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP3190733.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14850..15161)
                     /gene="rplU"
                     /locus_tag="IT904_000909"
     CDS             complement(14850..15161)
                     /gene="rplU"
                     /locus_tag="IT904_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP3190734.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15420..16391
                     /gene="ispB"
                     /locus_tag="IT904_000910"
     CDS             15420..16391
                     /gene="ispB"
                     /locus_tag="IT904_000910"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAP3190735.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16620..16898
                     /gene="sfsB"
                     /locus_tag="IT904_000911"
     CDS             16620..16898
                     /gene="sfsB"
                     /locus_tag="IT904_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAP3190736.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16946..18205)
                     /gene="murA"
                     /locus_tag="IT904_000912"
     CDS             complement(16946..18205)
                     /gene="murA"
                     /locus_tag="IT904_000912"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP3190737.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18260..18514)
                     /gene="ibaG"
                     /locus_tag="IT904_000913"
     CDS             complement(18260..18514)
                     /gene="ibaG"
                     /locus_tag="IT904_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAP3190738.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18674..18967)
                     /gene="mlaB"
                     /locus_tag="IT904_000914"
     CDS             complement(18674..18967)
                     /gene="mlaB"
                     /locus_tag="IT904_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAP3190739.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLVKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18967..19602)
                     /gene="mlaC"
                     /locus_tag="IT904_000915"
     CDS             complement(18967..19602)
                     /gene="mlaC"
                     /locus_tag="IT904_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAP3190740.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19621..20172)
                     /gene="mlaD"
                     /locus_tag="IT904_000916"
     CDS             complement(19621..20172)
                     /gene="mlaD"
                     /locus_tag="IT904_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAP3190741.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20177..20959)
                     /gene="mlaE"
                     /locus_tag="IT904_000917"
     CDS             complement(20177..20959)
                     /gene="mlaE"
                     /locus_tag="IT904_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAP3190742.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20967..21776)
                     /gene="mlaF"
                     /locus_tag="IT904_000918"
     CDS             complement(20967..21776)
                     /gene="mlaF"
                     /locus_tag="IT904_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAP3190743.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21986..22963
                     /locus_tag="IT904_000919"
     CDS             21986..22963
                     /locus_tag="IT904_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAP3190744.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22977..23963
                     /gene="kdsD"
                     /locus_tag="IT904_000920"
     CDS             22977..23963
                     /gene="kdsD"
                     /locus_tag="IT904_000920"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAP3190745.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23984..24550
                     /gene="kdsC"
                     /locus_tag="IT904_000921"
     CDS             23984..24550
                     /gene="kdsC"
                     /locus_tag="IT904_000921"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAP3190746.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24547..25122
                     /gene="lptC"
                     /locus_tag="IT904_000922"
     CDS             24547..25122
                     /gene="lptC"
                     /locus_tag="IT904_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAP3190747.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            25091..25648
                     /gene="lptA"
                     /locus_tag="IT904_000923"
     CDS             25091..25648
                     /gene="lptA"
                     /locus_tag="IT904_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAP3190748.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25655..26380
                     /gene="lptB"
                     /locus_tag="IT904_000924"
     CDS             25655..26380
                     /gene="lptB"
                     /locus_tag="IT904_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAP3190749.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26428..27861
                     /gene="rpoN"
                     /locus_tag="IT904_000925"
     CDS             26428..27861
                     /gene="rpoN"
                     /locus_tag="IT904_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP3190750.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27884..28171
                     /gene="hpf"
                     /locus_tag="IT904_000926"
     CDS             27884..28171
                     /gene="hpf"
                     /locus_tag="IT904_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAP3190751.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28289..28780
                     /gene="ptsN"
                     /locus_tag="IT904_000927"
     CDS             28289..28780
                     /gene="ptsN"
                     /locus_tag="IT904_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAP3190752.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28826..29680
                     /gene="rapZ"
                     /locus_tag="IT904_000928"
     CDS             28826..29680
                     /gene="rapZ"
                     /locus_tag="IT904_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP3190753.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29677..29949
                     /gene="npr"
                     /locus_tag="IT904_000929"
     CDS             29677..29949
                     /gene="npr"
                     /locus_tag="IT904_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAP3190754.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30163..30795
                     /gene="yrbL"
                     /locus_tag="IT904_000930"
     CDS             30163..30795
                     /gene="yrbL"
                     /locus_tag="IT904_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAP3190755.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30792..31520)
                     /gene="mtgA"
                     /locus_tag="IT904_000931"
     CDS             complement(30792..31520)
                     /gene="mtgA"
                     /locus_tag="IT904_000931"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAP3190756.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31517..32170)
                     /gene="elbB"
                     /locus_tag="IT904_000932"
     CDS             complement(31517..32170)
                     /gene="elbB"
                     /locus_tag="IT904_000932"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAP3190757.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32400..34736)
                     /gene="arcB"
                     /locus_tag="IT904_000933"
     CDS             complement(32400..34736)
                     /gene="arcB"
                     /locus_tag="IT904_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAP3190758.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34832..35761)
                     /locus_tag="IT904_000934"
     CDS             complement(34832..35761)
                     /locus_tag="IT904_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP3190759.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36436..>38973
                     /gene="gltB"
                     /locus_tag="IT904_000935"
     CDS             36436..>38973
                     /gene="gltB"
                     /locus_tag="IT904_000935"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAP3190760.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIA"
BASE COUNT         9739 a        10467 c         9446 g         9321 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccacgcca cattttgtca
      121 acgtttattg ctaatcatgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttctcct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tctggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctccact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgaaaacc gtatcataga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgcctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aacctggata tcactgatac ccgcgagaaa cttttcggtt atgccaaaac
     1621 tggcctgctt tcctcctctg ccgcttcagg cgtgccgcag gtggagaatc tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata gttgagattg
     2761 aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa cgctttttta
     2821 ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga aatgacaatc
     2881 agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattact
     2941 gccaaaaaaa gaagggataa aaaagcaaag gcacatagat aagggatata catccccagc
     3001 attttgacaa cttcatccgg atcactctgg agcacactaa tcgcaaatcc atcattgaaa
     3061 gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc agtcagtaac
     3121 acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt tacaaccagg
     3181 atggtcagaa cgaagaacag caagcgctga accatatgcg cgccggaaga ggcgactaag
     3241 ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt aaaacttcta
     3301 gcaaatttgt tgaatactgt catccagaaa tccaagttca caaccaatat aaacaattat
     3361 caacaatgtt caattttata tcagacatgt ttttcaactc tgatccatat cagaccgaaa
     3421 cactgttagt tttttctttt taaatgatgc ggccattgcg ttctcagtaa ttaaataaca
     3481 ttatcaatgc gtctctaaac attacgcaga acgtagccat cagtagttat actgacagga
     3541 gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt accggaactg gagttgacac
     3601 aataaagtgc caattatgtc agcagaaggg aaaagtttat aactaaggcg taccggcacc
     3661 atcgtttcaa ggtaccagct acgagtaaag caactggacg agatacagat acctgacaac
     3721 cattcctcag accaggacca aaacgaaaaa agacgctttt cagcgtctct tttctggaat
     3781 ttggtaccga ggacgggact tgaacccgta agccctattg ggcactacca cctcaaggta
     3841 gcgtgtctac caattccacc acctcggcac ggatactact ttttagttcg ggatatcgct
     3901 ggtcggctta gccggagcag ctggttgagt ttgttcggtt ttcaccggtg cactcagatt
     3961 ttcccattcg ctacctttat tggttttgtt gctattgatg ttacccagca ccagactgat
     4021 gatgaagaat aacgttgcca gcagcgccgt catgcgggtc atgaagttac cagaaccact
     4081 tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag gctcccatat cagcgccttt
     4141 accttgctgc agcatgatca gaccaacaag gccaattgcc acaataagga aaactactaa
     4201 aagagcttcg tacataatca acctgttcct tgcggagttg ccgcgtacca atgcttcaac
     4261 caataaagcg ggagttttta atctttccca ctgaagcggg tgtgaatact aaccaaagcg
     4321 aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa
     4381 ccgatgtgtt cgctgagaaa aaaggcgaca atcgccgcct ttttagccag ttatctaacg
     4441 ctttaaacgg cttttactgc atcggcgatg cggtgtgcaa attcagtcac ctgcgcttcg
     4501 tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc cggatttacg cagcaacacg
     4561 cgtccacggt tgcccagcgc agcttcaacc tctgcggtca cggctttaac tgactcatgc
     4621 tcaagtggat cgccgctacc tgcggtgtaa cgtacgttaa ccagaatctg cgggaacatt
     4681 ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac gtgccatagc cgccagcacc
     4741 tgcaagccag caacgatgcc gtcaccggta gtagttttat ccagcaggat cacatgaccg
     4801 gaattctctg caccgatacg ccagcctttc tcctgcattt tttccagtac gtagcggtca
     4861 cccactttcg cgcgcgcaaa tggaattcct aactgtttca gcgccagttc aagccccatg
     4921 ttgctcatca atgtacccac agcgccacca cgcagctggc cctgacgaag accttcacgc
     4981 gcgatgatat acatgatctg atcgccatcg actttattgc cttcatggtc aaccataatc
     5041 acgcgatcgc catcgccgtc gaaggcaata ccgagatccg ctttttcagc cagcacacga
     5101 gcctggagcg cgcgaacgtc ggtagccccc acttcggcat tgatgtttac accgtttggc
     5161 tcacaaccga tagcgataac gttcgccccc agttcgcgca gcacgttcgg cgcgatgtga
     5221 taagtcgcac cgtttgcaca atccaccaca atcttcagtt cactgaggct aagttcgttc
     5281 gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcgg catcaacgat acggctggct
     5341 ttacccagtt ctgccgaatc aacgcagctg atctcctttt ccatttccgc ttcgatggcc
     5401 tcttctaccg catccggcag tttggtgccg tcgatagaga agaatttaat gccattatcg
     5461 tagaacgggt tatgcgatgc agatatcaca attccggcct ctgcgcggaa ggtacgcgtc
     5521 agataagcca cggccggtgt tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc
     5581 gccagacccg cttccagtgc tgactccagc atatagccag aaatacgcgt gtctttacca
     5641 ataatgatct tacgggagcc gtggcgcgcc agcactttac ccgcggccca acccagctta
     5701 agcacaaaat caggtgtgat cggcgcatcc cctacacgac cacgaatccc atcggtaccg
     5761 aaatatttac gattactcat agcgtttgtt ttcctttgca gacagagtgg cttccaccac
     5821 ccgcatcgct tctacggttt ctttgacgtc atgaacacga ataatgtgcg cgccttgcat
     5881 tgcggcaatg accgcacagg ccagactacc gctcaggcgc tcggacggcc ccacgttcag
     5941 cagctgccca atcatcgatt ttcgtgacat acctaccaac agcggcaggt tgaaatggtg
     6001 aaattcagcc aggcgcgcca gtaatgaata gttatgggag agatttttac cgaaaccgaa
     6061 tccggggtcg agcaacaatt tctcttttgc gatacccgcc tgctcgcaac gtgctatttg
     6121 ctcaataaag tagcgattca cttctgcaaa gacatcgtca tacttcggag cttcctgcat
     6181 ggtttttgga tttccctgca tatgcatcag acaaaccggt aaaccggttt ctgcagccgc
     6241 ctccagagcg ccaggttcgg aaagggagcg gatatcatta ataatgtgag cgccaacttt
     6301 cgctgactca cggatgactt ctggtttgga tgtatctacc gagatccaga cttcgaagcg
     6361 ttgagcaatt gcctcaacca caggaataac acgttgcaac tcttcttcaa cgctaacttc
     6421 cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat
     6481 catcagattc gcatgtttca ccgcatctat cagcgagtta tgcgtgccac catccgaaaa
     6541 ggaatcaggc gtgacgttga ggatccccat tacgtgagga tggctaaggt ccagtgaagt
     6601 accctgggca aagagtttca tgatgttatc cctagtatga attgataaga aaaaaccccg
     6661 gagcacgccc cggggttttc ggtacaaata cagtcatctg atgcgggaac ttacttgtcg
     6721 cctaactgct ctgacatggt gttacccggg ttcggcgtac gcggttcatc aaccggacga
     6781 ggagcctttg gactaccatt gtcgccagaa ttgttagaag cgcctggttc ttcccagccc
     6841 gctggcggac gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc
     6901 tcatatttca tgagagcatc tttcatcgca tgcagaatat ccatattgtc ggtcagaagc
     6961 tgacgcgcac gattatagtt acgctcaatc agtgctttca cttcctggtc gatgatacgt
     7021 gcagtttcat cggacatatg tttcgctttc gctacgctac ggccgaggaa cacttcacct
     7081 tcttcttccg cgtacagcag tggacccaat ttctcagaga agccccactg agtcaccatg
     7141 ttacgtgcca ggttggtcgc aactttaata tcgttggacg caccggtaga tacatgttcc
     7201 ggcccgtaga tgatctcttc tgccagacga ccaccgtaca gcgtagaaat ctggctttcc
     7261 agtttctgac ggctggcgct gattgcgtcg ccctcaggca agaagaaagt cacacccagc
     7321 gcacgaccgc gtgggataat cgtcactttg tgcaccggat cgtgttccgg caccaggcga
     7381 ccgataatcg catgacccgc ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc
     7441 accatggagc gacgttccgc acccatcatg attttgtctt tcgctttctc gaactcaacc
     7501 atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt caccaggttc
     7561 gccaggtcag caccggagaa accaggagta ccacgggcaa tgattgccgc gtcgatatcg
     7621 ggtgccaatg gtacgcgacg catgtgaact ttcaggatct gctcacgacc gcgaacatct
     7681 ggcaagccga ccacaacctg acggtcgaaa cggccaggac gcagcagggc cgggtcgaga
     7741 acgtccggac ggttagtcgc ggcgataacg atgatacctt cgttaccttc gaagccatcc
     7801 atctcaacca gcatctggtt cagagtctgt tcacgttcat cgtgaccacc gcccagacca
     7861 gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa agatgatgca cggtgccgct
     7921 ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac ccacaccgac gaacatttct
     7981 acgaagtcag aaccggagat agtaaagaac ggaactttcg cttcgcctgc aatcgctttc
     8041 gccagcagcg ttttaccggt acccggagga ccgaccatca agacgccttt cgggatctta
     8101 ccgccgagtt tctggaagcg gctcggctcg cgcagatact caaccagttc agcaacttct
     8161 tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc
     8221 agcatgcgcg ctttgctctt accaaacgac atggcacctt tgccaccgcc gccctgcatt
     8281 tgacgcatga agaagatcca gacaccaatc agcaacagca tcgggaacca ggagatgaag
     8341 atagaagcca gcaggcttgg ttcttcaggc ggttcaccga caaccttgac gttcttggtc
     8401 aacaggttat ccagtaattt cggatcctga accggaatgt aagtggtata acggttacta
     8461 tctttcttgg taacgttgat ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta
     8521 ttgacctctt gtaggaaggt agagtaatcc accttacggc cattagactc gctgggccca
     8581 aagctctgga atactgacat cagcacaacg gcaatgacca gccagagtat taggtttttc
     8641 gccatgtcac tcaagggatt aacctcttat tacaactgtg ttaaaaacag cgtcaggata
     8701 ctctatatcc agcatctttc aaactttcgt ctgaaatctc ccggttaggg tttacgcccg
     8761 gtcgctacaa tatacacttc ccgcgaacgt gcacgagaag agtccggctt acgaactttg
     8821 accttcgtaa acagggagcg aatttccctt agatactcat cgaaaccttc gccctggaac
     8881 accttcacta caaaactgcc acctggcgct aatacatcac gacacatttc tagcgccagt
     8941 tccaccagat acatggcacg ggggatatcc accgccggtg ttccgctcat gtttggtgcc
     9001 atatcggaca tgacaacctg gactttgctg tcgccaacgc gctccagcag tgctttcatc
     9061 accagttcat cacgaaaatc gccctgaaga aagtccacac caacgatagg atccataggt
     9121 aaaagatcgc aagcgatgat gcggcctttg ccgccaattt gggtgaccac atattgtgac
     9181 caaccacccg gagcagcacc aaggtcgaca accgtcattc ccggtttaaa gagtttgtca
     9241 ctttgctgta tttcatcaag tttaaaccag gcacgggaac gtaacccctt tttctgtgcc
     9301 tgttgaacat atttatcgct aaagtgttcc tgaagccagc ggctggagct ggcagaacgc
     9361 ttcttacctg tcatttaact ttcccatggg gataactcat cgtaaccaat tgcgtaaatt
     9421 tttacgcgcc tatttggtga tatatgggag atggcggtag aatgacccgt tttcaatccc
     9481 aacgtaagca aaaaatacga tgaatctgag tactaaacaa aaacagcacc tgaaaggtct
     9541 ggcacatccg ctcaagccag ttgttctgct tggcagtaat ggtttgaccg aaggggtgct
     9601 ggccgagatt gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa tcgccaccga
     9661 agatcgcgaa actaaaacct tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa
     9721 tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca actaaagaac gtaaaatctc
     9781 gctgccacgc taagattatc ctaaagttac acacatttgc tgtgtaaaac gaggggtttt
     9841 ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga taaaaggccg catagcggcc
     9901 tttttccttt ctttacaata catcaacatc ttgagtattg ggtaattctt acaggtattc
     9961 caccttaatt acttcaaatt ctacttcgcc gcccggcgtt ttgatgacca caacatcatc
    10021 ttcttctttg ccgatcaggc cacgagcaat aggcgagttt acagaaatca ggttttgttt
    10081 aaagtcagct tcgtcatcgc caacgatgcg ataagtctgt tcttcgtcag aatcgagatt
    10141 cagcaccgtt acggtagcac caaaaataac gcgcccattg ttgggcattt tggtgacatc
    10201 aatcacctgc gcgtttgaca gcttggcttc gatgtctttg atacggcctt cgcagaagcc
    10261 ctgctgttca cgagctgcgt ggtattcggc gttttctttc aggtcgccat gctcacgcgc
    10321 ttccgcgata gcagcaatga tttcaggacg gcgcacagat ttcagaaaat ccagctcttc
    10381 gcgtaatttt tcagcgccgc gtaaggtcat cggaatagct tgcatttgtt atacctcttg
    10441 aatattcctg atggggcaag tcttcaccca ccccggctgt tcggcctgcc cggcataact
    10501 cctcaccggg accagaagca aaaaaatacc gacccgggta caagtcccag gtcagctaca
    10561 attcacattt tgatagtcat tttaccctga agttcccgaa gggtcatcgt ttactttata
    10621 gggcgttgcg ccgtagtatg acggctcgat tccaggttgt tagcgcgaga ttatgcgatt
    10681 ttccagattt atcatcggat tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt
    10741 tgatgagtat attactcaac tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt
    10801 cggcgcgtcg gcccccgcta ttgattacca cagtcagcag atggcgctgc ccgccagtac
    10861 gcagaaagtg attactgcgc tggcggcgtt gattcaactc ggccccgatt ttcgttttac
    10921 cacgacgctt gaaaccaaag gcaatgtgga aaacggcgta cttaagggtg acttagtggc
    10981 gcgatttggt gccgatccga cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt
    11041 gaaaaaatct ggcgtcaacc aaatcgatgg caatgtgttg atagatacct ccattttcgc
    11101 cagccacgat aaagcccccg gctggccatg gaatgacatg acacaatgct ttagcgctcc
    11161 gcctgccgcc gccatagttg accgcaactg tttctccgtc tcgctctaca gtgcccaaaa
    11221 acccggtgat atggccttta tacgcgtggc atcttattac cccgttacga tgttcagcca
    11281 ggtacgcacc ctcccccgtg gttctgccga agcgcaatac tgcgaactgg atgtggtgcc
    11341 aggcgacctg aaccgcttta cgctgacggg atgcctgcca caacgttctg agccgctccc
    11401 gttggctttt gccgtacagg atggagccag ctatgccggg gcaattctga aagatgagtt
    11461 aaaacaggcg ggtatcacct ggagtggaac actgctgcgc cagactcagg ttaacgaacc
    11521 tggaacggta gttgccagta aacagtcggc cccgctgcac gatctgctta agattatgct
    11581 gaaaaagtca gacaacatga tcgccgatac ggttttccgc atgataggcc atgcgcgctt
    11641 caatgtgcct ggaacatggc gggccgggtc ggacgccgtg cgtcagatcc tgcgccagca
    11701 agccggtgtc gatattggaa acaccattat tgccgatggt tcagggcttt cgcggcataa
    11761 cctgattgcc cccgccacca tgatgcaggt gctgcaatac attgcccaac acgacaatga
    11821 acttaacttt atctccatgc tgccgctggc gggctatgac ggctctttgc agtaccgtgc
    11881 aggtctgcat caggcgggcg tggatggcaa agtctcagcg aaaaccggtt cgttgcaggg
    11941 ggtatataac ctggcgggat tcattaccac agcgagcggg caacgaatgg cgtttgtgca
    12001 atatctttct ggctatgcag tagaaccggc ggatcagcgt aatcgccgta ttccgttagt
    12061 gcgtttcgaa agccgtttgt ataaagatat ttaccagaac aattagtcaa aaagaaaccc
    12121 cggcatatgg ccggggtttc agattattga caaagtgcgc tttgtttatg ccggatgcgg
    12181 cgtgaacgcc ttatccggcc tacaaaatcg tgcaaattca atatattgca atcatcttgt
    12241 aggcctgata agtgtagcgc atcaggctta tttggcattg gtcattagtg attaacgctt
    12301 gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag tcgtcatccc agtcctcatc
    12361 atcctcttca gcaatctctt caagctgctg gcgatgataa tcatcccaca tgaattcgac
    12421 tttctctggc tgtttcgctt cttcagcctg cacgaccggg ttttcaatga taaaggtcat
    12481 cacatcccag cagagatctt tcacgcccag tccgctcgcc gcagagatca gataatattt
    12541 atcttcccag cccagcgcct cagcgatcgc tttcgctttc tcttcggctt ctgccttatc
    12601 cagcagatcg atcttgttga acactaacca acgcggtttc gccgccagat cctggctgta
    12661 tttttccagc tcgctgataa taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg
    12721 atcgatatcg atgaggtgca acaggacgcg gcaacgttcc aggtgcttca ggaagcggat
    12781 acccagacct gcgccttccg cagcgccttc aatcagtcct ggaatatcgg caacaacgaa
    12841 gctcttttcg ttgtccattc gtaccacacc cagacttggc accagagtgg taaacggata
    12901 atccgccact ttcggtttag ccgccgatac cgcgcgaata aaggtcgatt tccccgcgtt
    12961 tggcatcccc aacataccga cgtcagccag cagcatcagc tccagcagca gctcacgctt
    13021 atcacccggc gtaccgttgg ttttctgccg tggtgtacgg ttaacggacg atttgaaacg
    13081 ggtattgccc agaccgtgcc agccgccctt agcaaccagc agacgctgac cgtgtttagt
    13141 catatcgccc atggtttcac cagtaccctg gtcgattaca cgcgtaccta ccggcacttt
    13201 aatcgtcacg tctttaccgc gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg
    13261 ctctgcacgg aaagattttt caaaacgata atcgataagc gtgttcaggt tttcgtcggc
    13321 ttccatccat acgtcaccac catcaccacc gtcgccgcca tccgggccgc ctttcggaat
    13381 atacttttcg cggcggaagc tcacgcaacc attaccgcca tcacctgcaa cgaccagaat
    13441 cgatgcttca tcaacaaact tcattttatt ctccgtaaat cattcgcctg cgcggggttg
    13501 cgataccacc gttgtatgct tacgtaatcc gccccaaata cgatgaccaa tggcggaata
    13561 catcgcgccc gcaaccacga caaacgcacc gagataacct aaaaggttta acatcggtct
    13621 ggcgaagaaa tcgggccagg ccagtgataa aagatctgaa aaaaacagcg taaacagtgg
    13681 ggtgagcgtg atgatcgcgc tcacctgcgc tgcctgccag cgagccatcg cttccgccag
    13741 ggcgccatat cctaccaatg tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg
    13801 gctaagctgc gctatcactc caggctttgc cagagggaag agcgcgattg tacataaagt
    13861 gtacagcaaa aacaggatct gcggtgaggc cagccgacgc aataaaacct tttgcgccac
    13921 gccataactc acccaaaccg tcgccgcacc gaccccaaag atgactcccc aggtgtaatc
    13981 agtgagcttt gtaaatatct cgaccagact ggtgttaaaa aacatcacca ggccgctcag
    14041 gagcatcaat gcccctacaa cctgagtgct gcgcattttc tctttcagga taaatacgct
    14101 ggcaaccatc atgccaactg gcgagagttg cccaatcacc tgcgaagcgg tcggactcag
    14161 gtattgcaag gatgagctga acaggatgaa gttcccaaac agcccggcgg tcgccactgc
    14221 caacaaaatc aaccagcgtg gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac
    14281 cgcaagaatg gcacccaggc caatactcgc catcaagaaa cggtaaaaca cgattgtcgg
    14341 aggttccatc acctccagca cctgcttcat tgcgattggc aacgcccccc agcaaattgc
    14401 tgtggtgagc gccaaaagaa tgccaatgcc tgcctgctgc ttcataccgt attccctgca
    14461 aagcgcattt gcggattaac gtcgcaccat gctcattttt accggttatc gaatgtaaaa
    14521 agccccgcaa cgtgttgcgg ggctttcatc cgttaccggg acgcgaaaaa cttattcagc
    14581 ttcgatgctg ataaatttac ggtttttcgg gcctttaact tcgaatttca ctttaccgtc
    14641 tgctttagca aacagagtgt ggtcacgacc gcaacctacg ttagcgccag cgtggaattt
    14701 ggtaccacgt tgacgaacga tgatgctacc cgccagaacg gattcgccac cgaaacgctt
    14761 aacgcccagg cgtttagctt ctgaatcgcg accgttacgt gtggagccgc cagccttttt
    14821 atgtgccatt tgaaatctct cctcaggtct taggcgctga tgccagtaat tttcacatca
    14881 gtgaaccact gacgatggcc ctgctgctta cgatagtgtt tacgacgacg aaacttaacg
    14941 attttaactt tctcgccacg accgtgagca acaacttcag ctttgattac gccgccatca
    15001 acgaaaggaa cgccgatttt gacttcttca ccgtttgcga tcatcagcac ttcagcgaac
    15061 tcaacagttt cgccagttgc gatgtccagc ttttccaggc gaacggtctg accttcgctt
    15121 actcggtgtt gtttaccacc actttggaaa accgcgtaca tataaaactc cgcttccgcg
    15181 cacacctttt caatgattca gagtgcgcta taaatattca caatagggcg cgaatattac
    15241 gcaaaacgca cgcctttgac aagtgctaca gtcaatacac gaagaaaaaa aacacaactt
    15301 gtacggtaac gtttatctgt gccatttttt cagtacaatc accctatatt cctaaccata
    15361 aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg gcttttgcga
    15421 tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa
    15481 tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat tacatcgtca
    15541 gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga gctgttggct
    15601 atgagggaaa tgcgcatgtc accattgctg ccttgatcga gtttatccac acggcgactc
    15661 tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct accgccaacg
    15721 ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc cgcgctttcc
    15781 agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa gccgtaaacg
    15841 tcatcgcaga aggtgaagtt ctgcaactga tgaatgttaa cgatccggac atcactgaag
    15901 aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt
    15961 gttccggaat tctggctggc tgtacgccgg aggaggagaa aggcctgcag gattatgggc
    16021 gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat gccgatggcg
    16081 aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc
    16141 tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc gccatcgaac
    16201 agggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct tgtggatctc
    16261 ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca gcgttacagg
    16321 tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc gctgttcaac
    16381 gcgatcgtta atcccctccc ctcatcccgc gcagtgcgcg ggatgattcc agtaaactcc
    16441 ataagaatac ttattcagcg taataatgca ctgaaaaacc agtgtataca tctttatata
    16501 ttctgaatat tcacactctt tacaggaact ttttagagca atcggccatc aggagtatag
    16561 tgatgctcga cagaagaagt gttctgaatg aaagcgacta acttaaggag tgaggaaaga
    16621 tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcgggtttg cgcaagaagg
    16681 gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg ctggcgaatg
    16741 cattatcgcg cccatggccg aaaggagaga tgattattgc gaaggccctg ggaactgacc
    16801 cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt atcgacagaa
    16861 cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg tagccccgcg
    16921 tacggggctg ccagctttca gacgattact cgcctttcac acgctcaata tttgcaccta
    16981 aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa atacgatcaa
    17041 ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat gcacgcagat
    17101 cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata acggtattgc
    17161 tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg cacatgcata aagcggtttt
    17221 caaagaccgt ttcggtgatg aatccagtcc cttctgccac caggttcaac agcgtgaact
    17281 gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta acagccttcg
    17341 gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg tccgctccag
    17401 cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta cggcagataa
    17461 ttttgccgcg agaaatcgcc gccgccacca ggaatgtacc ggtttcgata cgatccggca
    17521 gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata cgatcggtgc
    17581 cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg
    17641 gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc cagggttgca gcacacatga
    17701 tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct ttcagacgac
    17761 catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct aattgttcga
    17821 ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca ggtagtgaaa
    17881 cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa gcacgcatgg
    17941 ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca atatgcacag
    18001 aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat gtatcgacgt
    18061 ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtaga gcggcaaaaa
    18121 ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg
    18181 gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc atatccgcta
    18241 ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag
    18301 gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat tccatcagcg
    18361 gaccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac tcacccacgg
    18421 caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg gagagagcgt
    18481 tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc agttaataaa
    18541 ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag cctcgctgat
    18601 aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa cgccatatcc
    18661 ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat ttcgccaggg
    18721 tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt ttcaccagat
    18781 cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggagaca cggctaagat
    18841 cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt agcaaaacgt
    18901 cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag ctcagtgact
    18961 cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt tcagttgcgc
    19021 agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt gtttggtggt
    19081 gatcatactg acgccttcag caatcatgtc gtaagcctgc caattgcccg tctgggagtt
    19141 tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa taatggtaac
    19201 gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct gataggtttg
    19261 accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac ggaaagcggc
    19321 aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca gcaccagcgc
    19381 accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg tacgcagata
    19441 atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg ttttctgcgc
    19501 cgcctcgtcc atcagcttat acggattggt ctggtctgcc gcggttgccg cactcagagg
    19561 tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa
    19621 ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag cagctggcgc
    19681 atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac caatgagatc
    19741 ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca ggatagcagt
    19801 ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc ccagcaggcc
    19861 ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt gttcaatttc
    19921 cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca cccgacccac
    19981 aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt
    20041 cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg ccttcaggca
    20101 aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa tttcattttt
    20161 tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca
    20221 gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc ccggcagacg
    20281 tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg gcgaacacca
    20341 cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt tgcattgccg
    20401 accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat ccgccccaga
    20461 tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca gcccagaaac
    20521 ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg gagagttgct
    20581 ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc ccggcaaaca
    20641 acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc agcataccca
    20701 gactggtttc cgcactataa gtggtcagaa ccagataacc ttgcagcccc aacaccattc
    20761 cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca ttatagagct
    20821 ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc gcattgaata
    20881 acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt ccgagcgacg
    20941 ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc gtgataatcg
    21001 ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg
    21061 cgcggatcag gattcgcctg taacgcctgg gcactgccat gagcgacaat ttttttgtcc
    21121 gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg agaaaccacc
    21181 acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag tacgcccatg
    21241 gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg ctccagcgca
    21301 atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga aggcattagt
    21361 ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact atgcaacaat
    21421 ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata
    21481 tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac tgtatacagg
    21541 cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc atgatctggt
    21601 gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga tggccccatg
    21661 atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat gcagcgattg
    21721 ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg ctccataatt
    21781 cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt gaacccaaca
    21841 tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt actttttagc
    21901 cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag cggaccggcg
    21961 agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa ttgttggttt
    22021 acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac
    22081 ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta catcgttacc
    22141 agaagtcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac
    22201 agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg cgctggttcg
    22261 tccttttacc gtccattctg atgttctacg ccgtgaatta cccttaatgt tgttggtcag
    22321 cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg gtatctttct
    22381 cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg
    22441 tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg acggcggatt
    22501 gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg ccacgcggat
    22561 ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt tgacgatggg
    22621 tctgacggca attgctatcg gaaccagcct gccggaactg gcaaccgcaa tagcgggggt
    22681 tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat
    22741 tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc cactggcgta
    22801 cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc tgtgctggcg
    22861 gcgctccccg caaccgggcc gtggtgtagg ggtattatta actggcggat ttatcgtatg
    22921 gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa cgcattatgt
    22981 cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa gtcctggcga
    23041 ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc acgcttgcct
    23101 gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatggga aaatcggggc
    23161 atattgggcg aaaaatggcg gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc
    23221 atcctggtga agccgcgcat ggtgatttag gcatggttac cccacaggat gtggtgattg
    23281 ctatctctaa ctctggtgaa tccagcgaaa tcacggcctt aattccagtg cttaagcgtc
    23341 ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg cgcgccgcag
    23401 atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg gcaccgacca
    23461 gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac
    23521 gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg ggtcgtaaac
    23581 ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat gttaagaaaa
    23641 cggccagtct gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt atgactgtca
    23701 tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta cgccgtgtct
    23761 tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg ccggggggaa
    23821 tacgtgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag tcccgccata
    23881 tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat atgcatgatt
    23941 tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca aagcaggtgc
    24001 gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atggcaaaag cagagaacat
    24061 tcgtctgctg atcctcgacg ttgatggcgt actgtcagat ggcctgattt atatgggcaa
    24121 taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct
    24181 cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg tagaagatcg
    24241 ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt
    24301 tagcgatctg ctggaaaaac tggcgattgc cccggaaaat gtggcttatg tcggcgatga
    24361 tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca
    24421 tccactgttg atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt
    24481 gcgcgaagtt tgcgacttat tactcctggc gcagggcaaa ctggatgaag ccaaagggca
    24541 atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggc ggttctggtg
    24601 atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt caacaacaat
    24661 gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga aggggcacta
    24721 agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt ttcgtggttt
    24781 acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc cgtaaaagca
    24841 gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt tgaagtcaac
    24901 gcacttgtgc cggactctca acttcgcaga atcacgacgg ataacgcgca gatcaatctg
    24961 gtgacgcagg atgttacctc tgaagacctc gtcacgttat acggaacaac atttaactcc
    25021 agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat tgaaaaggtt
    25081 agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc ttgccagctc
    25141 acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc agccgatcca
    25201 cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct ttaccggtaa
    25261 tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc
    25321 gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa cgttctacca
    25381 gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact acgaactggc
    25441 aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata gcaacattaa
    25501 gggcgataag atcacttacc tggtgaaaga gcagaaaatg caggctttca gcgacaaagg
    25561 caagcgcgta accaccgttc tggtgccgtc gcagctgcag gataaaaaca acaaaggcca
    25621 gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg caaagaacct
    25681 tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg
    25741 ggaaatcgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt tctacatggt
    25801 tgtaggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg atatcagtct
    25861 gctgccactg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg aagcctccat
    25921 tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc gtgacgactt
    25981 gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc acattgagca
    26041 cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc
    26101 ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg ccggggttga
    26161 cccgatctcg gttatcgaca ttaaacgcat cattgagcac ctgcgcgaca gcggcctggg
    26221 cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac gcgcttatat
    26281 cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac aagacgaaca
    26341 cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa gtttgcgacg
    26401 ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag gcttagccaa
    26461 caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg
    26521 gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac
    26581 actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacgctgga caccgccgac
    26641 gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg ggacaccatt
    26701 tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgatga gctgccggtc
    26761 tatcagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt cgagctgaca
    26821 ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt tgatgacacc
    26881 ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga agagatcgac
    26941 atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt cggtgtagcg
    27001 gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa aaccacgccg
    27061 tggctggaag aggccagatt gatcattagc gatcatctcg atctgttagc caatcacgac
    27121 ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat
    27181 ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga acctgagtat
    27241 gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacagtaga actcaacagt
    27301 gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa taacgctcgc
    27361 aatgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg gttgatcaag
    27421 agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag
    27481 caagccttct ttgagcaagg cgaagaatat atgaaaccga tggtactggc cgatatcgcc
    27541 caagctgtcg aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa atacctgcat
    27601 agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa taccgagggc
    27661 ggcggcgaag cttcctccac ggcgattcgt gcgctggtga agaaattaat cgcggcggaa
    27721 aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga acaaggtatc
    27781 atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc gccgtcaaac
    27841 cagcgtaaac aactcgtttg acccaaccga taaggaagac actatgcagc tcaacattac
    27901 cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcca aatttgccaa
    27961 acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag tggagaaagt
    28021 cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg ccagcgcgga
    28081 aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac gccagttgac
    28141 caaacataaa gataaactga aacaacacta attgtccggg caattagcat gtgcatggcg
    28201 gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct gttccactgt
    28261 ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc tacagcttag
    28321 cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc
    28381 cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctgccgcctc aggtggtttt
    28441 tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg gtattgcaat
    28501 tccgcatggc aaactggaag aagatactct gcgtgccgtt ggcgttttcg ttcagctcga
    28561 aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt ttgccctgct
    28621 ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg cgaaacgtct
    28681 ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag agctgtatca
    28741 aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta atgtctcttt
    28801 tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac gttcaggttc
    28861 aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg tggataacct
    28921 tcccgtagtg ttgttacccg atctggctcg aaccctggcc gatcgcgaga tttctgccgc
    28981 cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac aggcgatgag
    29041 taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc gtaatacctt
    29101 aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa acctgtcgct
    29161 ggaaagtgct atcgacaaag aaagcgattt gctggagcct ctgcgttcgc gagcggatct
    29221 gattgtcgat acctcagaaa tgtccgttca cgagctggct gaaatgctgc gtacccgtct
    29281 gctgggtaaa cgtgaacgcg aactgaccat ggtctttgag tctttcggct tcaaacacgg
    29341 tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc cgcactggga
    29401 tccgaaactg cgtccaatga caggtcttga taagcctgtc gccgcgttcc tcgaccgcca
    29461 cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat ggttacctat
    29521 gctggaaacc aacaaccgta gctacctgac ggtcgccatt ggttgtaccg gcgggaagca
    29581 ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg gtaaaaacgt
    29641 ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca aactgttgaa
    29701 atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga attaatgcag
    29761 ggttttgacg ctgaagtgct cttacgtaat gacgaaggca ccgaggctga agccaacagc
    29821 gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt tgaagcgacc
    29881 ggtccacagg aagaggaagc actggccgcc gttatcgccc tctttaattc tggttttgat
    29941 gaagattaat cttcatcact ttgacataca actcccttca aactcccccc gacaataaga
    30001 aaatcacgta ctgaaatcgt tctcaatcaa cgtcatttgt acattttgtg cgcttttcac
    30061 tttcagaaga accttaagaa aaccttaaga ggcattgttt aggttttgtt taagttaatc
    30121 gaccatactg gagatcgtca gaaaatattt ccaggagatg gcatgattcg cttatctgaa
    30181 caaagtcccc tgggcaccgg aagacatcgg aaatgttatg cgcatccgga agatgcccaa
    30241 cgctgtatca agattgtcta ccatcgtggc gatggtggcg ataaagagat ccgccgtgag
    30301 ttaaagtact acgcgcatct tggtcgccgg ttaaaagact ggagtggaat accgcgctat
    30361 cacggtaccg ttgagacgga ttgcggaacc ggttatgtct acgatgtgat tgcagatttt
    30421 gacggcaagc cttccattac gctgaccgaa tttgctgaac aatgccgtta cgaagaagac
    30481 attgcacaac tgcgccagct actgaaacag ctgaagcgtt atttgcagga taaccgtatc
    30541 gtgacgatgt cgctgaagcc gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt
    30601 atcccggtcg tctgcgacaa cattggtgaa agcacattga ttccactggc gacctggtcg
    30661 aagtggtgtt gtctgcgtaa gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg
    30721 ctggctattg ccctgcagaa agatctgcaa cctcgtgaga gcaaaacgct ggccctcact
    30781 tcgcgcgaag cttaatccag ctggtgttgt tgcataaacg gctcaccgcc taactgatac
    30841 atctgccgta aaatccacgc ctgacggcta cgcacgtagc ccgatggcga ggagactttg
    30901 aaacgaagtg gattaggtaa tacagctgcc agtaatgcag cttccgaccg ggtaagtttg
    30961 ctcgcgggtt tgtggaaata acgttgtgcc gcagcttcga cgccaaacac accgtcgcca
    31021 aattcggcga tattcaggta aaccgtcagg atacgctttt tgctccagac cgtttctatc
    31081 cccagcgtta atccggcttc cagccctttt cgaacccagc tacgcccatc ccataaaaag
    31141 agatttttgg ctgtctgttg agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc
    31201 tcgttgtgcg ccagggcttt ctcaatggaa gcgacatcaa agccccagtg ctcaggaaat
    31261 ttctgatctt ctgcggcaat caccgccagt cccatccacg gcgagatttg atccatactg
    31321 acccagtcag aatgtgccac gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc
    31381 tcgaccatta ccgctgagaa gggaacaggc gcaacgctaa acaacgcgat gcccccgccc
    31441 cagaaaacgg cgaggacaac cattaaccgc aaaagaaaac ggcgaacgaa actaaacacc
    31501 gttaagcggc ttttactcat tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc
    31561 cgctcgccgc ttctgcaatg ttctgcgcca gcatatatgc tggggtggtg acaatcttat
    31621 tgtcttcatc aaccacgata tcatcgacag gacacggcac atgctccgcg cccatctctt
    31681 ccagcacttc tgcggtatcg atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa
    31741 ttttcggcag catcgccggg gcaatacaca taaaaccaag cggttttccg gcctgatgca
    31801 tcgcttgtgc cagcgccttt aattcacggt caacggtgca ttcgctacca agactggcaa
    31861 aattgcttaa attcttcgcc gcgccaaacc cccccggcac aatcaacgca tccagttcag
    31921 cggcatcggc ctgggccaga ggacggattt caccgcgcgt tattcgtgcc gcttcaatca
    31981 gcacattgcg cgtttccgtc atcgcttcgc cagttaaatg gttgataaca tcaacctgct
    32041 gcttatccgg tgcaaagcag accgcctgcg caccgctgcg tgaaatagct aacaacgtca
    32101 acaccgcttc atgaatttca gaaccgtcat agacgccgca tccgctcaga attacgccaa
    32161 ttttcttcat tgtgatcatc ctttcgcaat tgactgaaac acattaattt ttttaataaa
    32221 aatggtacgc atcacacatt taactgattc atgtaacaaa tcatttaagt tttgctatct
    32281 taactgcgtg cggcctgaaa aacagtgctg tgcccttgta actcatcata ataatttacg
    32341 gcgcagccaa gatttccctg gtgttggcgc agtattcgcg caccccggtc tagccggggt
    32401 cattttttag tggcttttgc cacccacgct ttcagcactt ctacgtcgtg acgccactct
    32461 tctttcatct cttcaatcca ttcaccgacg ttatcttccc aggccggaag gtcaggagac
    32521 tgaatttgct gacccagctg ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta
    32581 attttatgtc cttcctcaac aatgcctttt ttatcctgcg ccgtcagatt cgactccagc
    32641 acgctgacat agcccggcat catcttctca aacaccgcta acccgtcggt gatcagcttc
    32701 ggtcctacaa gttcgagata ctgttccagc atgggaatat cgagcaatgc ttctgattta
    32761 ctgttctctt ctgtcgtcac cgtactctcc tcatcatcct gggtatccca gaatttcttg
    32821 atcatcgcgg ttagcgccgg aacagaaagc ggcttactca gcacatcatc cattccagca
    32881 ttgaggtact cttgtttgtc tttcagcacg ttagcggtta aggccaccag cggcggtaaa
    32941 tcctcgcgcg gataacgttt cgtcagttca cgagagatat ccagcccggt catatctggc
    33001 aactgaatat ccagcaacac caggtcgtat tcgcccggtt taaacatctc cagcgccgcc
    33061 ttgccggtca tggcgacatc aacgctgtta cctaattttt ccagcacaga acgcgcaaca
    33121 atcacgttca gttcaatgtc ttccaccagc agcacattca gcgccggtaa aggcatatcg
    33181 tcttcatcaa acgcatcatc tacctcttct gctaccgacg gtgcgtggat cgtcaacgta
    33241 aaggttgaac ctttgccctg ttcgctggta accgtaatat cgccgcccat atttttcgcc
    33301 agacgacgag aaacggccag accaataccg gtgccggtgg caggtttacc gccatgactg
    33361 tctttcacct ggtaatacat ggcgaaaatt ttatccagct catcctgcgg aatgccgata
    33421 ccagagtctt ccacttcaaa atgcagcata tcgccttcat cgtagcgcac gcgcacggta
    33481 acctggcctt gctgggtgaa tttgacggcg ttactgatga ggttccacag gatctgccgt
    33541 aaacgcgtcc cgtcggtaat gacctgatgc ggtaatggca gcgtcggctc caggttaaag
    33601 cgcaatcctt tttgttgcgc ctgcaatgcg gagagatttt ccagatcggc aaggaagctg
    33661 gtgaaatcaa ccggttgatt atcaagctgg accttgcgcc gttccatctt atccatgtcg
    33721 ataatatcgt taaagatatt ccccagcgtg acggccgaaa catggatggt cttgagatat
    33781 ttttcctgct cggcggtgag ttcggtatcc agcagaatgc ggctcagacc gacgataccg
    33841 ttcagcggtg tacgcaattc gtgactgatg gtggagataa acgtcgtttt gtcgcggctg
    33901 gcccgttcaa gcgcatcctg ataccgctta cgctcggtaa tgtcgcgacc aaagcccatc
    33961 aaaccgtgac gtttacccac gcggtcgtag tacggcactt tacggatttc aaagcaggct
    34021 ttgcgcccgt ccgggtaatc cagccactgt tcataggtca gtgacacatt atgacggaac
    34081 actttttcat cggtttcaat gacttttgcg gcggcttccg gtgagtaaac atcagcaggt
    34141 ttcaggtgaa ccagttgttt ttcgcttttt ccggtcagca gctccatcgc gcggttacag
    34201 ccggaaaact ctttatcttc gttacgataa aaaaccaggt cgggtgaagc atcaaggaag
    34261 gaacgtaaga atgaggattg ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt
    34321 tcaattttca gttggccgaa ggtttcctgc agttctgctt ccgctttttc acgaacggca
    34381 atttcctgat ttagctgggc aatattatct tttaactgaa cgttgaggct caaatcgcgc
    34441 tcgcgcatct cctccagttt ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt
    34501 tgctcgacga ccaccgatag aaagtagacc gcccacggcg taatcagcaa accaaagaag
    34561 atagaacgaa taacatcaat gctttcgacc tgaccatgca gcaccatggt taccgccatt
    34621 tgtaccacaa tggcaagaac gacgagggcc agcgccagca acattgagaa gcgcaccaga
    34681 cctaacttca tcatcaggtc aacataatac tgcgccagca gacgaatttg cttcattagg
    34741 gaattccttc acgacaacct gccccaataa tacccaattc tgacagttac gttataaatt
    34801 gtgtgagaaa tgcggaattc ccctcaacga cctactccgt tggaggtagc cacggacgtc
    34861 ccagcgccga tccctgtacg ccatgttcat tcagatagcg atccagctcg accattcccg
    34921 tccatcgatt ttcgcaccac agcggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa
    34981 tgcggtggta gatcacttcc ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt
    35041 aatcctccag ttcaataccg ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc
    35101 ctttcacaat atgcagcgga tgcagcttta tgccgtccac gccggtttca accactcgct
    35161 ccagcgtttg caaacattca gcctgccctt cgccaggcag gccgacaatt aagtgggaac
    35221 acaccttcag cccgcgctga cgtgccagct gggttgtacg ctgataacag gcaaaatcat
    35281 ggccgcggtt gatgcgatgc agtgttttgt cgtgggcggt ttgtagcccc agctccagcc
    35341 acacttcgta accctggtcc ttatattcgc aaagcagatc cagcaccgca tccggcacgc
    35401 agtccgggcg ggtacccaca cacaaaccga caatattggc ctggctcacc gcctgctgat
    35461 acatagaacg cagcacctga acttccgcaa aggtgctggt atacgcctga aagtaggcca
    35521 gatagcgttt agcgcggtta actaaattcg cctggtgcgc cagttgctcg gcaatggaac
    35581 gatgctgctg cgcttcatcg gcaaacgagg caacattaca gaatgtgcag ccaccacgcc
    35641 cgatggtgcc gtcacggtta gggcagctaa aaccgccgtg cagcgtcagc ttatgcacct
    35701 tttgcccata acgacgggtg agatcaccac caaacatatt gactaatttc tgtaactgca
    35761 taatctgata gaccgcgcct tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg
    35821 cgatgacctg gatcaatcgt cccggcctgc ttttatcaac tgcataatca atcaaaatta
    35881 ccgaaatttc atgcataatc acataaatca cttttgctta tcttgtgtca gattttttta
    35941 tctcctgatg gattttaggc aaaaacagta gcatgaaacg tcattaccaa ttaaggcagt
    36001 ataaaatgct ggttttgtcg tcagttcaag gcaggataag ggttaacaca cctttatgac
    36061 agtcaggaat tgactgtttc tctaacgact tcccttttag ccttaaagat aaaatccatt
    36121 ttaatttcag tcatttaata aagaattttg cgctaaagca catttctgta ccaataagct
    36181 tgccatttga cctgtatcag ctttcccgat aagttggaaa tccgctggaa gctttctgga
    36241 tgagcagcct gctcatcata tttatgcagt aattgagatc ccctcttcac cgtattaacc
    36301 gatgcgaaaa ggacaacaag ggggcgaatg cgaggcgcgc gtatgacacg caaaccccgt
    36361 cgccacgctc tttctgtgcc cgtgcgcagc ggttcggaag tggggttccc gcagagcctg
    36421 ggggaggttc acgatatgtt gtacgataaa tcccttgaga gggataactg tggtttcggc
    36481 ctgatcgccc acatagaagg cgaacctagc cacaaggtag tgcgtactgc aatacacgca
    36541 ctggcccgca tgcagcaccg tggcgcgatt ctcgccgatg gtaaaaccgg cgacggttgc
    36601 ggcttgctgt tacaaaaacc ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg
    36661 cgtttagcaa aaaactacgc tgtcgggatg ctcttcctga ataaagatcc tgaactcgcc
    36721 gctgccgcac gccgcatcgt tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc
    36781 tggcgtgatg tccccactaa cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgcca
    36841 cgcattgagc aaatttttgt gaacgccccg gcaggctggc gtccacgcga tatggagcgc
    36901 cgtctgttta tcgcccgccg ccgcattgaa aagcgtctcg aagccgacaa agacttctac
    36961 gtctgtagcc tgtcgaatct ggtgaacatc tataaaggtc tgtgtatgcc gacggatctg
    37021 ccgcgctttt atctggatct tgcggacctg cgtctggaat cggccatttg cctgttccac
    37081 cagcgcttct ccactaacac cgtaccgcgc tggccgctgg cgcaaccgtt ccgctatctg
    37141 gcgcataacg gtgaaatcaa caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtact
    37201 tataaattcc agacaccgct tatccctgac ctgcacgacg ccgcaccgtt cgtcaacgaa
    37261 accggctctg actccagttc gatggataac atgctggaac tgctgctggc aggcgggatg
    37321 gatatcatcc gcgccatgcg tctattagta ccacccgcct ggcagaacaa cccggatatg
    37381 gacccggaac tgcgtgcctt ctttgacttt aactccatgc atatggagcc gtgggatggc
    37441 ccggcgggca tcgtgatgtc cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt
    37501 ctgcgtccgg cgcgctacgt catcaccaaa gataagctca tcacctgcgc ctctgaagtc
    37561 ggtatctggg attaccagcc tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa
    37621 ctgatggtta tcgacacccg cagtgggcgt attctgcact cggcagaaac cgatgacgat
    37681 ctgaaaagcc gccatccata taaagagtgg atggagaaaa acgtccgccg actggtaccg
    37741 tttgaagatc tgcccgatga agaagtgggt agccgcgaac tggacgacga cacgcttgcc
    37801 agctaccaga aacagtttaa ctacagcgcg gaagagctgg actccgtaat tcgcgtactg
    37861 ggcgaaaacg gtcaggaagc ggtcggttcg atgggcgatg ataccccatt cgccgtgctc
    37921 tccagtcagc cgcgcattat ttacgactac ttccgccagc agtttgccca ggtgactaac
    37981 ccgccaatcg acccgctgcg tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt
    38041 gaaatgaacg tcttttgcga agcagagggc caggcgcacc gtttaagctt taaatcgccg
    38101 attctgctct actccgattt caaacagctc acgacgatga aagaggagca ctaccgcgca
    38161 gatacgctgg atatcacctt tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag
    38221 ctgtgcgaca aagccgaaaa aatggtacgt agcggcaccg tgctgctggt gctctccgac
    38281 cggaatatcg ctaaagatcg cctgccggtt ccagccccga tggcggttgg cgcgatccag
    38341 acccgtctgg tcgatcaaag cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc
    38401 gcccgcgatc cgcaccactt cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca
    38461 taccttgcct atgaaacgct gggccgcctg gtagacaccc atgcgattgc caaagattat
    38521 cgtaccgtga tgctcaacta ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc
    38581 aaaatgggca tctccaccat cgcctcttac cgctgctcga aactgtttga agcggtcggt
    38641 ctacacgatg atgtagtggg cctgtgcttc cagggggcgg tcagccgcat tggtggggca
    38701 agctttgaag acttccagca ggatctgctg aatctgtcga aacgtgcctg gctggcgcgt
    38761 aagcccatca gccagggcgg cctgctgaaa tacgtccacg gcggcgaata ccacgcctac
    38821 aacccggacg tggtgcgcac gctgcaacaa gcggtacaaa gcggcgagta cagcgactat
    38881 caggaatacg cgaagctggt taatgagcgt ccggcaacca cgctgcgcga tctgctggca
    38941 attacgccgg gtgaaaacgc ggtcaacatt gct
//
LOCUS       DACAPB010000020        38519 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.020, whole genome shotgun sequence.
ACCESSION   DACAPB010000020 DACAPB010000000
VERSION     DACAPB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.020"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..390
                     /gene="citX"
                     /locus_tag="IT904_000936"
     CDS             <1..390
                     /gene="citX"
                     /locus_tag="IT904_000936"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP3190761.1"
                     /translation="EVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIA
                     APARDLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCA
                     RGKTHQLTDLLNRMEALLNDVDACNVN"
     gene            365..1243
                     /gene="citG"
                     /locus_tag="IT904_000937"
     CDS             365..1243
                     /gene="citG"
                     /locus_tag="IT904_000937"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP3190762.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            1294..2757
                     /gene="citT"
                     /locus_tag="IT904_000938"
     CDS             1294..2757
                     /gene="citT"
                     /locus_tag="IT904_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAP3190763.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            2871..3677
                     /gene="rna"
                     /locus_tag="IT904_000939"
     CDS             2871..3677
                     /gene="rna"
                     /locus_tag="IT904_000939"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAP3190764.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAIALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            3906..4316
                     /gene="rnk"
                     /locus_tag="IT904_000940"
     CDS             3906..4316
                     /gene="rnk"
                     /locus_tag="IT904_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAP3190765.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(4547..5785)
                     /locus_tag="IT904_000941"
     CDS             complement(4547..5785)
                     /locus_tag="IT904_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAP3190766.1"
                     /translation="MKALTYHGPHHVQVENVPDPGVEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLHFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLSFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAGRGYEIFEKREEECRKVILVPGAQN
                     AEAAQKAVSGLVNAMPGGTI"
     gene            6006..6434
                     /gene="uspG"
                     /locus_tag="IT904_000942"
     CDS             6006..6434
                     /gene="uspG"
                     /locus_tag="IT904_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAP3190767.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(6555..8120)
                     /gene="ahpF"
                     /locus_tag="IT904_000943"
     CDS             complement(6555..8120)
                     /gene="ahpF"
                     /locus_tag="IT904_000943"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP3190768.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(8365..8928)
                     /gene="ahpC"
                     /locus_tag="IT904_000944"
     CDS             complement(8365..8928)
                     /gene="ahpC"
                     /locus_tag="IT904_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAP3190769.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            9300..10046
                     /gene="dsbG"
                     /locus_tag="IT904_000945"
     CDS             9300..10046
                     /gene="dsbG"
                     /locus_tag="IT904_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAP3190770.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKEIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            10255..11157
                     /locus_tag="IT904_000946"
     CDS             10255..11157
                     /locus_tag="IT904_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3190771.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            11304..12524
                     /locus_tag="IT904_000947"
     CDS             11304..12524
                     /locus_tag="IT904_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP3190772.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPLQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            12497..13126
                     /locus_tag="IT904_000948"
     CDS             12497..13126
                     /locus_tag="IT904_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP3190773.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(13127..14287)
                     /gene="ybdL"
                     /locus_tag="IT904_000949"
     CDS             complement(13127..14287)
                     /gene="ybdL"
                     /locus_tag="IT904_000949"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAP3190774.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAHHVAQGANQYAPMTGVQTLREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            14396..15484
                     /gene="hcxA"
                     /locus_tag="IT904_000950"
     CDS             14396..15484
                     /gene="hcxA"
                     /locus_tag="IT904_000950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAP3190775.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFKKVESFKA"
     gene            complement(15494..15691)
                     /locus_tag="IT904_000951"
     CDS             complement(15494..15691)
                     /locus_tag="IT904_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAP3190776.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(15874..17979)
                     /gene="cstA"
                     /locus_tag="IT904_000952"
     CDS             complement(15874..17979)
                     /gene="cstA"
                     /locus_tag="IT904_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAP3190777.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(18160..18573)
                     /gene="entH"
                     /locus_tag="IT904_000953"
     CDS             complement(18160..18573)
                     /gene="entH"
                     /locus_tag="IT904_000953"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAP3190778.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTVVLG"
     gene            complement(18576..19322)
                     /gene="dhbA"
                     /locus_tag="IT904_000954"
     CDS             complement(18576..19322)
                     /gene="dhbA"
                     /locus_tag="IT904_000954"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAP3190779.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(19322..20179)
                     /gene="entB"
                     /locus_tag="IT904_000955"
     CDS             complement(19322..20179)
                     /gene="entB"
                     /locus_tag="IT904_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAP3190780.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(20193..21803)
                     /gene="entE"
                     /locus_tag="IT904_000956"
     CDS             complement(20193..21803)
                     /gene="entE"
                     /locus_tag="IT904_000956"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAP3190781.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALIEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(21813..22988)
                     /gene="entC"
                     /locus_tag="IT904_000957"
     CDS             complement(21813..22988)
                     /gene="entC"
                     /locus_tag="IT904_000957"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAP3190782.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            23363..24319
                     /gene="fepB"
                     /locus_tag="IT904_000958"
     CDS             23363..24319
                     /gene="fepB"
                     /locus_tag="IT904_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP3190783.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTQAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(24323..25573)
                     /gene="entS"
                     /locus_tag="IT904_000959"
     CDS             complement(24323..25573)
                     /gene="entS"
                     /locus_tag="IT904_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAP3190784.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRHFRQTPPQVTASDS"
     gene            25684..26688
                     /gene="fepD"
                     /locus_tag="IT904_000960"
     CDS             25684..26688
                     /gene="fepD"
                     /locus_tag="IT904_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAP3190785.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            26685..27677
                     /gene="fepG"
                     /locus_tag="IT904_000961"
     CDS             26685..27677
                     /gene="fepG"
                     /locus_tag="IT904_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAP3190786.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQTALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            27674..28489
                     /gene="fepC"
                     /locus_tag="IT904_000962"
     CDS             27674..28489
                     /gene="fepC"
                     /locus_tag="IT904_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP3190787.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(28486..29619)
                     /gene="fepE"
                     /locus_tag="IT904_000963"
     CDS             complement(28486..29619)
                     /gene="fepE"
                     /locus_tag="IT904_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAP3190788.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKSFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(29835..33716)
                     /gene="entF"
                     /locus_tag="IT904_000964"
     CDS             complement(29835..33716)
                     /gene="entF"
                     /locus_tag="IT904_000964"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAP3190789.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAMQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQLNATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGTFEKIGPIIRATLNR"
     gene            complement(33713..33931)
                     /locus_tag="IT904_000965"
     CDS             complement(33713..33931)
                     /locus_tag="IT904_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAP3190790.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCVLPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(33934..35136)
                     /gene="fes"
                     /locus_tag="IT904_000966"
     CDS             complement(33934..35136)
                     /gene="fes"
                     /locus_tag="IT904_000966"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAP3190791.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            35379..37619
                     /gene="fepA"
                     /locus_tag="IT904_000967"
     CDS             35379..37619
                     /gene="fepA"
                     /locus_tag="IT904_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAP3190792.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            37794..38414
                     /gene="entD"
                     /locus_tag="IT904_000968"
     CDS             37794..38414
                     /gene="entD"
                     /locus_tag="IT904_000968"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAP3190793.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT         9255 a        10464 c         9832 g         8968 t
ORIGIN      
        1 gaggtcacac gccgaatttt taatcatggc gtgacagcct tgcgtgcctt agccgcaaaa
       61 cagggctggc aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg
      121 ttgagcattg ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt
      181 catcctctcg ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc
      241 cgccgcgact attcactgcc gcctcgccgc tgcctgttgt gcgagcaaag cgcagccgtc
      301 tgcgcgcgtg gaaaaaccca tcaactgacc gatttactca accgcatgga ggcactgctg
      361 aacgatgtcg atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt cattaatcga
      421 tgagtacgcc ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc
      481 aggcctcgtg gatcgcatta actgcggtgc gcacaaagat atggcgctgg aagatttcca
      541 ccgcagcgcg ctggcgattc agggctggct accccgtttc attgaatttg gtgcctgtag
      601 tgcggaaatg gcaccagaag cggtactcca cggattacgc ccaattggta tggcttgcga
      661 aggtgatatg ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt
      721 agggctgcta tgtgcggcaa ttggccgttt gcttcaactc aaccaaccgg taacgccaac
      781 aaccgtttgt tctacggcgg caagtttctg ccgtggcctg accgatcgcg aactgcgtac
      841 caataattca caactgacgg caggtcaacg gttgtaccaa cagcttggcc ttaccggcgc
      901 acgcggtgaa gccgaagcgg gttatccact ggtgatcaat cacgccttgc cgcattacct
      961 cactctgctg gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact
     1021 gatggcgatc aacggcgata ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg
     1081 gctacagcgc gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccgccgatct
     1141 cgattatctc cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag
     1201 tgccgacctg ctgatcctta cctggttttt agcacagatt taattattta agcacttgat
     1261 aaatttggaa atattaattt tcggagaacc cgtatgtctt tagcaaaaga taatatatgg
     1321 aaactattgg ccccactggt ggtgatgggt gtcatgtttc ttatccctgt ccccgacggt
     1381 atgccaccac aggcatggca ttacttcgct gtgtttgtgg caatgattgt cggcatgatc
     1441 ctcgagccaa ttccggcaac agcgatcagt tttattgcgg ttactatttg cgttattggc
     1501 agtaattacc tgctctttga tgccaaagaa ttagctgatc cagcgtttaa tgcgcaaaaa
     1561 caggcgctga aatggggtct ggctggtttt tccagcacca cggtatggct ggtatttggc
     1621 gcatttattt ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc
     1681 ctggtgaaat tcatgggcaa acgcacgctg acgttgggtt atgcaattgt cattatcgac
     1741 attctgctgg caccgtttac accgtccaac accgcgcgta ccgggggtac ggtttttccg
     1801 gtcattaaaa acctgccgcc gctgtttaaa tcattcccca acgatccgtc cgcgcgtcgt
     1861 attggcggct atttgatgtg gatgatggtc attagtacca gtctgagttc gtccatgttt
     1921 gtcaccggtg cggcaccaaa cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc
     1981 cagattagct ggttgcagtg gttcctctgc ttcctgccgg ttggggttat cttgcttatc
     2041 attgcgccgt ggctttccta cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg
     2101 gcaacctggg cgggtgatga actaaaaacc atgggtgcgc tgacacgcag agagtggacg
     2161 ctgattggcc ttgtattgct cagcttaggt ttgtgggtat ttggcagtga agtcattaat
     2221 gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg ctctgcacgt tgtgccgtgg
     2281 aaagacatta cccgctataa cagcgcatgg aacacgctgg tcaacctggc aactctggtt
     2341 gtgatggcta acggcctgac tcgttctggt tttattgact ggttcgccgg taccatgagt
     2401 acgcacctgg aaggattctc accaaacgca acggtgattg tactggttct ggtgttctac
     2461 tttgcacact acctgtttgc cagcctgtct gcgcacaccg caaccatgct gccggttatt
     2521 ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac aactgtgtat cctgctggtg
     2581 ctgtctatcg gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt
     2641 tacggctgtg gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat cttcggggtg
     2701 atttacatct ctatgttgct gttggttggc tggccgattc tcgccatgtg gaactaatac
     2761 gttcgccaaa cgctgaataa atcatcacgc ccgccaggta agcccaccta gcgggctttt
     2821 ttatgattta atagatagta ttcagtgtat gtatgagttc cacacccatt atgaaagcat
     2881 tctggcgtaa cgccgcgttg ctcgcggttt ctctgcttcc cttctcttct gccaacgcca
     2941 tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc
     3001 aaaccggatt ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc
     3061 tgcaaaccga aacgaccaac aaagctgatt ttctgaccgt acatggtctg tggccaggat
     3121 tgcctaaatc ggtcgctgcc cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg
     3181 ctactcgccc aatcccgaat ctaccagaag cgcgcgccag ccggatgtgt tcatcgccgg
     3241 aaaccggatt atcactggaa acggccgcta aactaagtga agtcatgcca ggagctggcg
     3301 gacgttcctg cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc
     3361 cggacgcata cttcggtact atggtacgcc tgaatcaaga aattaaagag agcgaagccg
     3421 gaaaattcct tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct
     3481 ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc cagggtaacc
     3541 ctgcgtattt gactgaaatt cagatctcga tcaaagctga cgccatcaac gctccgcttt
     3601 ctgcaaactc attcttgcca caacctcacc caggtaactg tggcaaaacc tttgtgattg
     3661 ataaagcggg ttattaatcc tctgatttac aaaacacgct aattcgtctt ccataaccat
     3721 gacgattaag aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat
     3781 cacttcaaag agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac
     3841 ggtgagggtt ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag
     3901 taaaaatgtc cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc
     3961 tgctggagca acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg
     4021 atcgcgccca aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc
     4081 gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg
     4141 caaaaatgac cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc
     4201 tggggctgcg cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc
     4261 ttgaagtgct ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc
     4321 atctgcacaa gcgataactc gctttgccca gaggatgcac tcgcatcctc tttacgcaaa
     4381 atcagcaatt tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt
     4441 tgtgggaata accatggcag aagcatttta catcctgata ggatttctga ttatggcggc
     4501 gattatcgtc atggctgtcc tttacctcga aaaccactcc tgacgatcat attgttcccc
     4561 ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ttttgtgcac
     4621 cgggtaccag aatcacctta cggcactcct cttcacgttt ttcgaaaatc tcatatcccc
     4681 ggccggcctc ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt
     4741 tctcaattaa cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa
     4801 acgacaaccc tttatcaaag gcatcgccaa acaggaaacc gtgaataaat ccagcgtaga
     4861 cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc aatacactga cgcaacgctt
     4921 taccgctgct gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg
     4981 cttcaaaacc gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa
     5041 taattgactg tgccggatcg ctgtcttcat caaaattaat cgggatcgcg ccgtagcggt
     5101 cggcggcgaa atgcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga
     5161 gcaaccgtgc acaggcgatt gtcaacaatc ccacaggacc agcaccatag actgcaacgc
     5221 ttgaaccttg ttggatctgc gcattttttg ctgcctgcca tgccgttggc agaatatcag
     5281 aaaggaaaag cgctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc
     5341 ctttagggac gcggacatat tccgcctgcc caccaggaac gccgccatac aggtgactat
     5401 aaccaaacaa tgccgctgga gctggtatct gttttttatt gagcgcagcg cctttacccg
     5461 cattggtatt ttcgcaggcg gcatattgtt gcaatcgaca gaaaaaacag tcgccacaag
     5521 caatgacgaa cggaattacc actcggtcgc ctttttgcaa attttttacg tcctttccgg
     5581 tttcaactac ttcccccata aattcatgac caaaaatatc gccatgttta acctgaggta
     5641 ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttgccgta atacgcagaa
     5701 taatatcatc tgcctgttca acgcccggat cgggaacatt ttctacctga acgtgatgtg
     5761 ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc
     5821 tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg gagaattcct
     5881 ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag aaccagacaa
     5941 atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa cagggagaaa
     6001 gggttatgta taagacaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca
     6061 aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg
     6121 tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg
     6181 aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc cacttcacca
     6241 tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg
     6301 agttggcaga agaactgggg gctgatgttg tagttattgg ttctcgcaac ccatcgattt
     6361 cgacccatct gttaggttct aacgcctcga gcgtgatccg ccatgccaat ctgccggtgc
     6421 tggttgtgcg ttaattgcca gaataaatat cccgccctgc ccggagccat cccggcaggg
     6481 ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg
     6541 aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag
     6601 gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt cgtacagtca
     6661 cccgctgcga atacgccttt gacgttggtt tcgcatttcg catcaatgat aatctcaccc
     6721 atgcggttac gttcgactgc gccttccagc cagttggtgt tcggcagcag accaatctgg
     6781 acgaaaatac cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc
     6841 agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg
     6901 tcgacgtttt tcaggctgcg cagtttgtcc tgcagcacct ggtcggcttt catttctggc
     6961 gcaaattcca gcagagttac gtgctcaacg atacccgcca ggtcgattgc cgcttccacg
     7021 ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg gccgtcgcag
     7081 tgcgggcagt aggtcacgcc tttggtgcga tactgatctt cgcccggaac gttcatgttg
     7141 cgccattttg cacctgtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct
     7201 gtttcaatct gatgcagacc accttcaact gctgctggga tcagtttgct ggcgctctgg
     7261 ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccagcttc
     7321 tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga
     7381 ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct
     7441 gccgctgcac ccgccgggcc ggaaccgacg attaatacgt cataagcatc acgcttgttc
     7501 agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat ttcagtcaac
     7561 gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac gcccatcacg
     7621 ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc
     7681 gggttcagta cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt gtggcaagag
     7741 agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga
     7801 gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac cagcgaggtg
     7861 aactcgtggc ccagcgggga gcctgcaaaa cgtggcccct ggttggaacc tgggttggtg
     7921 atcaggaaag acggcttacg caccggcaag ctgttatctt ctttaaaggt gactttgtct
     7981 gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc
     8041 agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt
     8101 ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc
     8161 tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag
     8221 cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc ggctagcctg
     8281 tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg ccaacgccgc
     8341 tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag acggagccag
     8401 agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag aagctacgta
     8461 ctgtgctgct ttgattttac gcagcaggtc agacgcgtca cggccaatgc cttcagcggt
     8521 aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac ggtcagccag
     8581 accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg ggtcgccgat
     8641 catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt tgtgggtgaa
     8701 gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt cttcgtagtg
     8761 gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg ggtagaagaa
     8821 gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt cgccgttttt
     8881 gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct atacttcctc
     8941 cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat gcgtttccat
     9001 tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga actttgcacc
     9061 gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa tctgaactac
     9121 cctttggtat cttcagtgcc tgcctgcagg ctgaggtgtt gcccttacaa atgcaacaac
     9181 gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag ctcttaccta
     9241 tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag gacaaattaa
     9301 tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca gaggaacttc
     9361 ctgctccagt aaaagagatt gaaaaacagg gcattacaat catcaaaaca ttcgatgccc
     9421 ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc atctacctga
     9481 ctccagatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt gaaaacctga
     9541 gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg tggcaacgga
     9601 tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt gtctacgtct
     9661 tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc ccgtgggtag
     9721 attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca gaaagcccgg
     9781 cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa caatatgaag
     9841 cctctggtgg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag caaatgaaag
     9901 tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg ccggctatct
     9961 attacatgag taaggaaaat acgctacaac aggccgtggg gttgcccgat cagaaaacgc
    10021 ttaatatcat tatggggaat aaataaaagg taattcatgg atggttgaac tatatccctg
    10081 cttttcaacc atcctaaaaa agcatgaatt gctcgctctg ccctaagtgg ctgtgctatt
    10141 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat atatacgcaa
    10201 aaaacattga ttaagtgaat atatcatgga agaaaaatat aaccggagta gtgtatggcc
    10261 aatctctacg acttgaaaaa gttcgactta aatcttcttg tcatatttga gtgtatttac
    10321 cagcatttaa gcattagcaa agctgctgaa tcactatata tcaccccatc agccgtgagc
    10381 cagtcgttac aacgcttacg tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa
    10441 ggtatcgcgc cgacgacaac agggttaaat ctgcatcacc atcttgagaa aaatctcaga
    10501 ggccttgaac aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc
    10561 tacggccctc agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa
    10621 gattcctctg tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa
    10681 ctgttggttc accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc
    10741 gtcatttgca tgccattgca tacgattcgc aatactctaa tttgtagtaa taggcatcca
    10801 cgaattactg acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata
    10861 tctaaatcag caggggttga tgatattcaa atggaaatag acgagagatt tatgaatcgc
    10921 aaaatctcat ttcgtggttc ctctttgctg acaatcatta atagcattgc agttacagat
    10981 ttactgggaa tagtccccta tgaactttat aattcctacc gtgactttct taacttaaaa
    11041 gagataaaac tagaacatcc gttaccttca ataaagcttt acatttccta taataaatct
    11101 tctttaaata atttggtgtt ctcccgattt attgaccgtc taaatgagag tttttgatat
    11161 ataactacag acagctacta taactttcat ctatttattc acagcgcgaa tgaaaaataa
    11221 aacactctgc tttacagtgt ttactttata attaacagac ttatctcact gatcaccctg
    11281 taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg ctcaatattc
    11341 tggaagctgc acgagagcgt attacctgga cactcaatac cctaccccgc gtttgtgtct
    11401 cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa cttgcccgac
    11461 aaatgggcaa aaaaatctgc gttttgttta tcgactggga ggcgcaattc tcttgcacca
    11521 ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag ttttactggg
    11581 ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa tggcagtgct
    11641 gggaacctga tgtcgaatgg gtacgtcaac ctctccaaga tgcgataacc gaccctgact
    11701 ttttctcctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag tttgccgaat
    11761 ggttttcaca aaaacgtccg gcggcgatga tgatcggcat ccgtgcggat gagtcctaca
    11821 accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat aaaccctgga
    11881 ctacggccgc accaggcggt catagctggt acatttaccc catttacgac tggaaagtgg
    11941 ctgatatctg gacctggtat gcaaatcatc aaagtctctg caacccactg tataacctaa
    12001 tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt ggcccggagc
    12061 aacgacaagg attatggctc tatcacgtta tcgaaccgga tcgctgggct gctatgtgcg
    12121 cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgacaat catttctatg
    12181 ggcatcggaa aatcctcaag ccagaacatt tagactggca agaatatgca ttattgctgc
    12241 tcaatagcat gccggaaaaa acagctgagc attaccgcaa taaaatcgcc atttatttgc
    12301 actggtatca gaaaaaaggc atcgaggttc cacaaaccca gcaaggggac attggcgcga
    12361 aagatatccc ctcctggcgg cggatatgca aagttttact caataacgat tactggtgtc
    12421 gggcattatc atttagcccc acaaaatcga agaactatca gcgttataac gaacggataa
    12481 aaggaaaacg tcaggaatgg gggatactat gcaacaacga ttaacgcagg atttaacgca
    12541 atttctcgcc agcttgccgg aagatgatcg catcaaagcg attaatgaaa tccgcatggc
    12601 gatccatcag gttagcccct ttcgtgaaga gccagtggat tgcgttctgt gggttaaaaa
    12661 cagccaactt atgcctaatg attacaaccc aaacaacgtg gcaccacccg aaaaaaaact
    12721 gctgcaaaaa tccattgaaa tagatggttt tacccaaccc attgtggtca cacatacgga
    12781 taaaaatgcc atggaaatcg tggatggttt tcaccgccat gaaattggta aaggctcatc
    12841 atcattaaaa ttacgcttaa aaggttattt gccggtgacc tgtctggaag gtacgcgtaa
    12901 tcaacgcatc gccgcgacta ttcgtcataa tcgcgctcgt ggacgccatc aaatcaccgc
    12961 catgtcggaa atcgtccgtg aacttagcca gttgggatgg gacgataata aaattggcaa
    13021 agagctggga atggacagcg acgaagtgct gcgcctgaag caaattaacg gcctgcaaga
    13081 gttgtttgcc gaccgtcaat attcccgcgc ctggacggtt aaatagctaa agctggcgca
    13141 ggcgttcagc tgctgccagc aacgtcgatt ccttcttggc aaaacagaga cgaatcagtt
    13201 tatgtgggaa gggatcggcg caaaacaccg acagcggaat cgccgctacg ccgtgctcct
    13261 gcgtcagcca ctggcaaaac tcaacatcat ccagggtaga aaccgcgctg taatccacca
    13321 gcaaaaagta tgtaccttca cacggtaaaa tctccagccg gctttcattt aaagcattca
    13381 ccagaatatc gcgcttctgg cgataaaagt ccggtaacgc aagataatgc tcaggttctg
    13441 cacgtagcat atcagcaagc gccagctgtg ccggggtatt caccgaaaag gtcagatact
    13501 gatgtacctt gcgaatttcg gcgctgatgg gcgctggcgc aacacaataa cccactttcc
    13561 agccggtcat atgataggtc ttgccaaatg aagaaaccgc cactgcccgc tcacgcagct
    13621 gcggatgcgc cagcacactg gcatggccct gttgtgaaaa gttgatgtgc tcgtagactt
    13681 catcgctaat gacaaaaatc tcgtgcccgg cgatcgcctg ccacaaagcg gcgaaatcag
    13741 cctgctgcca gacagttgca ctggggttat gcggagtgtt gaggatcacc agtctggtgc
    13801 gctcgcttaa taatgcggca aattcctgcc agtcaacgcg aaaatgcggt ggttgcagtg
    13861 ccatacgctt cactattccc ccagaaagcg cgatggcggg ggcgtaactg tcatagctgg
    13921 gatcaaaaca aatcacttca tcgccattgc gcaccagtgc ggtaatcgcc gcgtataacg
    13981 cttccgtcgc ccctgccgtt acggtgatat cgctatcggc atctggttga tagccataca
    14041 aacgttccgt tttctgagca atcgcctcgc gcaaggtctg cacgccggtc atgggcgcgt
    14101 attggtttgc cccctgtgca acgtggtgcg ccagccgctc ctgtaaatag cgcggaccat
    14161 caaaatcagg aaagccttgc gacaggttaa tcgcctggtg ttgctgcgcc agcgcgctca
    14221 tctgggtgaa aatagtggtg ccaagttgtg gaagtttgct ttgtggaatc agagggttat
    14281 ttgtcatttt tattgtacct gacgaagttg cgtgttgaag gcactataac acgatgctag
    14341 tatttggcaa tcaagacgtt tagatgtcta aataaaacaa taaggacaac acaacatgcc
    14401 tcacaatcct atccgcgtgg tcgtcggccc ggctaactac ttttcacatc caggaagttt
    14461 caatcacctg cacgattttt tcactgatga acaactttct cgcgcggtgt ggatctacgg
    14521 caaacgcgcc attgctgcgg cgcaaaccaa acttccgcca gcgtttggac tgccaggggc
    14581 aaagcatatt ttgtttcgcg gtcattgcag cgaaagcgat gtacaacaac tggcggctga
    14641 gtccggtgac gaccgcagcg tggtgattgg cgtcggtggc ggtgcactgc tcgacaccgc
    14701 gaaagccctc gcccgccgtc tcggtctgcc gtttgttgcc gttccgacga tcgccgccac
    14761 ctgcgccgcc tggacaccgc tctccgtctg gtataatgat gccggacagg cgctgcatta
    14821 tgagattttc gacgacgcca attttatggt gctggtggaa ccggagatta tcctcaatgc
    14881 accgcaacaa tatctgctgg cggggatcgg tgacacgctg gcgaaatggt atgaagcggt
    14941 ggtgctggct ccgcaaccag aaacgttgcc gctaaccgtg cgactgggga tcaataatgc
    15001 gcaagccatt cgcgacgtct tgttaaacag tagcgaacag gcgctgagcg atcagcaaaa
    15061 tcaacagtta acgcaatcat tttgcgatgt ggtggatgct attattgctg gtggtgggat
    15121 ggttggtggt ctgggcgatc gttttacgcg tgtggcggca gctcatgccg tgcataacgg
    15181 tctgaccgtg ctgccgcaaa ccgagaagtt tctccacggc accaaagtcg cctacggaat
    15241 tctggtgcaa agcgccttgc tgggtcagga tgatgtgctg gcgcaattaa ctggagcgta
    15301 tcagcgtttt catctgccga ctacactggc ggagctggaa gtggatatca ataatcaggc
    15361 ggagatcgac aaagtgattg cccacaccct gcgtccggtg gagtccattc attacctgcc
    15421 agtcacgctg acaccagata cgttgcgtgc agcgttcaaa aaagtggaat cgtttaaagc
    15481 ctgacgtaca aatttagcag cagcgcgcgc cgccttttcc accgtagcgc gcgtcctgcc
    15541 gctcacggaa aaactcttcg taggtcatcg gcgtttgatc gggatggtta acccgcatat
    15601 gttcgacata gttgtcgtaa tcaggcatac caatcatcag cttcgccgcc tgacctaaat
    15661 attttccggc ttttgccagt gaatcaaaca tcttggcctc cgctaaccat gttgctgaaa
    15721 gagtgccgga tgcggcgtga acgccttatc cggcctacaa atgctatcaa cgcgctcggt
    15781 cagtcccctc gccccaccgg ggagagggtt agggtgagag ggaaccctgg gcactaagcc
    15841 aacattcgcc aacatccccc cctcactctg actttagtgt gcgccttttg cctgcgccac
    15901 gatctcctcg acattttccg gcattggttc atacggcgtt tctttcgccg ttggcttcgg
    15961 atctttcaat gccgccagtg ccgtcttaat cgagaacagt gccagaacca ccacgaccac
    16021 cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt gcgccagctg
    16081 tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga acttattagc
    16141 aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag
    16201 ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac gttgacgttt
    16261 catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca tctggttggc
    16321 aataccaaac agcggccaca gagtgttaat gccgcccagc ggatcgacca cgccctgatg
    16381 gaggaagtag ccccacgcca gtacgcacag cgctgttgcc agcaggttag caggcagtga
    16441 atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca taaagcgcgc
    16501 agcacgcgta cctgcatcca ccgccgtcag aataaacagt gcttcaaaca aaatggcgaa
    16561 gtgataccag aacgccacat ccatcatgcc gcccagcgcg ccgtgcagaa tgtaggccat
    16621 ccccaccgcc agcgtcggcg caccgcccgc acgggaaatg atcgactgtt cacccacttc
    16681 gctggcaatc tggtttagcg tatctggtgt aatgctaaag ccccagctac tcaccacctg
    16741 cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt
    16801 catggcaaaa tacacgcccg gatcgatgat acaggcggaa accagcgcca taatcgccac
    16861 gaaggattcc attaacatcc caccgtagcc gataaagcac gcctgccctt cgttcgccag
    16921 catcttcggc gtggtcccag aagagatcag cgcatggaag ccagacaccg cgccacaggc
    16981 gatggtgata aacaggaacg ggaacaggtt accggtccat accgggccag tgccatcgac
    17041 aaatttggtc agcgcaggca tggtcagcgt cgggcgcata atcaaaatgc ctaccgccag
    17101 accgacgata gtcccgattt tcaggaaggt agagaggtag tcacgcgggg ccagcagtaa
    17161 ccacaccggc agcaccgccg ccacaaaacc gtaacccacc agcatccagg tcaactgcac
    17221 gccggtaaag tcaaagtacg gtgcccaggt cggactttct gccacccagc cgccagagat
    17281 aatggcgaaa atcaggaata ccagaccgat gaccgacact tcgccaatac gccccggacg
    17341 cagatagcgc aggtagatcc ccataaacag cgccagcgga atggtgaacg caacggtata
    17401 tgttccccac gggctatgag tcagggcttt caccacgatc atcgccagca ctgcaaggat
    17461 aatgaccatg atcataaagc aggccaccag cgcaatcacc ccggcggttg gccccatctc
    17521 ttctttgacc agctcaccca gcgagcgacc gtcacggcgc gtagaaacaa acagtaccat
    17581 gaaatcctgc accgcaccgg cgagaaccac cccagcgagc agccagatca tccccggcag
    17641 gtagcccatt tgcgccgcca gtaccggccc caccagcgga cctgctccgg caatggccgc
    17701 aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg
    17761 gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg cgatatacag
    17821 cccataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa tccacagcgc
    17881 gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc ccattacaga
    17941 gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc gtgtgcgtgt
    18001 ccatagagat gttacatttt gtttctataa catctttgta caatttaaca acacagcaac
    18061 catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga tcactccgtt
    18121 aaccgagttt tcctgcaatc tcatctaatt ctgtcgggtt catcccaaaa ctaccgtacc
    18181 cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgacgattt cccagctctg
    18241 attttgccga ccaagatgca gcggctggca gacgccgcgt acctttccct cagacaccgg
    18301 gcgatggtgt gttgcattaa gttctgtgcc taccacacac tgtccgtcgc gggtcatcat
    18361 aaatccggcc atcgatccca gcgtttccgc cagcgccgcc gacgcgccgc catgtagtaa
    18421 accgaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca catcatcgcc
    18481 cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg tggcgttcag
    18541 ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt tgagccgcca
    18601 tcgaccacaa tatcctgtag ggtaatatgg ctggcgaggt cagaggcgag gaacaaaatc
    18661 gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag tttaaactgc
    18721 tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc
    18781 atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct acccgccagt
    18841 tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc ataagcactc
    18901 atgccaatac gcggcgtgtg cgcggcgtcg gacgccacag tgacaatcgc cccgccccgc
    18961 tgacggcgaa actggttcat ggtttgctgg aacaggttaa acgcaccgcc gacgttaacc
    19021 gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat gcgtaaaatt
    19081 cccgccgcat tgaccagcgc gtccagtcgc tccgtttcag ctaacagtcg ctgacacact
    19141 tgcgcgacct gcgcagcgtc ggcaacatcc atcacttcgg tcgcaaaggg atattgctcc
    19201 tgagtgaacg cttgatcaaa acctgtaact ttcgctcccg cctcaacaaa cgccagcgcc
    19261 gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc gctgaaatcc
    19321 attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg tttttcgcca
    19381 gcatgacaaa gtcgatatca ccatgcactt tgcgccagcg cgccgccagc gccatcatgc
    19441 gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt
    19501 ccagcaacgg caggatcacc tcacgcagcg ccgctttgct ggcggggata ggtgctggca
    19561 gtaattcttc agtcatcacc acccggccag aacgtccggc cacatatttc agcgacatca
    19621 aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt ttaatatcgc
    19681 gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc ccggtaataa
    19741 tcagctggtt acgtccactc tctttcagca tttgctccag cggagaacga tgaaacgcgc
    19801 tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga tccaccacct
    19861 tttgctgttc cggcgagcgg gtcaggcccg gcccccacat atcattcaac agcgcccgat
    19921 cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg tgctgtttgc
    19981 agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg cagttctcgc
    20041 cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca cgttgcggtt
    20101 caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct
    20161 gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga cgccagccac
    20221 tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga atccacacac
    20281 tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa acgacgcacc
    20341 tgcaccgcgc gcagcggctc ttttaccacc agataagcgc agcttttttc gcccatcagc
    20401 tcatcttcca tgctcaccag tgcggcgtag atcaccgccg ggtggcgcag cagcaggttt
    20461 tcgatctctt cggcagcgat cttctcgccg ccacggttaa tctgatcttt ctcgcgcccc
    20521 tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca gtaaaaaccg
    20581 ttggcatcaa aggcgctggc attgtgctgt ggacttttgt aatagccgcg gaaggtgtac
    20641 ggcccgcgcg tcatcaggcg tccgacttcc ccttgcggca gtggatttcc ttcggcatcg
    20701 gcaacccata cttcgtcatc cggacacatt gggtaaccct gggtatggat aattttctcc
    20761 gcgctatcat caagtcgggt gtagttcacc agcccttccg ccatgccaaa cacctgctgc
    20821 aactgacagc caatctcagc gggaatacgc gccgcaaggg tggcagaaag acgtgcgccg
    20881 ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc gatcagcgcc
    20941 tgcaaccaca ggctgactgc gggtggcacc agcgcggtaa cgttaacctg atgtttttca
    21001 atcaatggga aacagagcgt ggcgctggga tcggccgcca gaacaaccgt tcctccggca
    21061 agaaagacgc ccagcgatcc tggcgaactc atggcgtagt tatgagccgc cgggatcgcg
    21121 cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg cacgctgtag
    21181 tagtagtcgt tatgagtgcg cgggatcagt ttcggtgtgc cggtggtgcc accggaaagc
    21241 tggaaatagg ccacttcatc agcaggtgat ggcgtggcag taaaatcctc agccggatgg
    21301 ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gcagttgcac cacgcgaatg
    21361 gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag cgcatgttgg
    21421 cgatcggcaa tcagcaatgc gggttcaatc tggctggcat aggcgttcag ttcactacgc
    21481 tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagcgc gaaaaaggta
    21541 atatacaatt cagcgacgtt acccagttgt accagcgcgg tttcaccagg tttaatgccc
    21601 tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg ataactcaac
    21661 tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag catgtcgcgt cagaatgtcg
    21721 gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa ctcttccggc
    21781 cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac gttcaacatg
    21841 gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc
    21901 cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg gatggtcacc
    21961 acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa cagttcgcgg
    22021 tcgaacggtt ccagttcagc aataacctgg gtcgcggcct gatgcgggaa accgctcagc
    22081 gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga attcgcttta
    22141 ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg tgatcagctg tggagaagaa
    22201 ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg agtcaccagt
    22261 tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacctgcttc gcgatcgagc
    22321 acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa acgctcgccg
    22381 tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc agccagcgga
    22441 acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa tacgccacta
    22501 tcaatggcgg cgtcagtggt gatatcaatc aaccgtgaca acaccacttt gtcgacctgc
    22561 ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt ttgctccgga
    22621 attgcctggc gttccaccac attcagcgac tggctgcggg tgaaacggcg tgcggaagct
    22681 tgtttttcct gacgggagaa cgactgccag gattcaggaa tatacagcga cgaaggctga
    22741 cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg cgctttggca
    22801 tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc cccgttcaca
    22861 gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta cggcgacata
    22921 aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc agccagtgac
    22981 gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc atttatattt
    23041 tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc
    23101 aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat tgatggattc
    23161 gcataagcgc aatgtgatgt cctgcgccgt tctgccccct ctcccttcca gggtgagggc
    23221 tggggtgagg gttaatgttc gcaccagtgc tggctgttcc cctcacccta accctctccc
    23281 caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc ccttaacctt
    23341 attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaacg cccttctatt
    23401 aacaggactt ttgctttcag gaatagccgc agttcaggcc gctgactggc cgcgtcagat
    23461 tactgacagc cgtggcacac atacactgga aagccagccg cagcgtattg tttccaccag
    23521 cgtcaccctg accggctcac tgctggcgat tgatgcacca gtgatcgcca gcggcgcgac
    23581 cacgccaaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga gcaaggtggc
    23641 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    23701 gcaaatgccg gatctgattt taattagcgc aactggcggg gattcggcgc tggcgctgta
    23761 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    23821 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    23881 tgcgcagttt gataagcaac tggcggcggc gaaagagcaa atcaaattac cgccgcagcc
    23941 ggtcactgcc attgtctata ctgccgctgc acacagtgcc aatctctgga cgccagaatc
    24001 agcacaaggg cagatgctgg aacaactcgg ctttacgcag gcgaagttac ccgcagggtt
    24061 aaatgccagc caaagtcagg gtaaacgcca tgacatcatt cagcttggtg gggaaaatct
    24121 ggctgcaggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga aagatgccga
    24181 tgctatttat gctaatccgc tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    24241 tgcgctggga accgagacgt tccgtctgga ttactacagc gccatgcaag tgctggatag
    24301 gcttaaggcg ctgttttaag cattaactgt cggacgctgt cacctgcggc ggcgtctggc
    24361 gaaaatgtcg caactccacc agcaccagca ataacaacac gccgataatc aacaaaccaa
    24421 aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc gcccaaacca cccagcagcg
    24481 ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca caaaccgtta atccgcccta
    24541 acatcgcttc cggggtttgc gtttgcagca ttgtgtattg cagcaacgag ctgaccgcac
    24601 tcaaccagcc gaacagcgcc agacaaacca cgcctaaaat ccacatcggc atcaggccaa
    24661 acagaccaat ggcgaggaac gatcccagcg tggagagcag catcaataac cctggtcgcg
    24721 cactatgtgc cagcttcccg ctggttaacg caccaatagc cgcgccgagc gggatcgccg
    24781 cgtagagaaa accaatctgt gccgctgaca tctgccagtt gtcagccagc gccggataca
    24841 gtacccgcac cgcgctcgcc atcgtcaata aaccacccag cagcgcaatc ccgcccacca
    24901 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcacgcg
    24961 gctgcggtgg cggtggcaac gccggaaggc ttaacaacgg tagcaaggta ataaacgtgc
    25021 ccgccgccgc cagcccgtag ttccaggcta cgccaccggt cgccagcaat aaaccgccaa
    25081 tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc ccggcctgca
    25141 ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgccgta acgccaagcg
    25201 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    25261 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    25321 tcactttttt gcgctcatag cgatccgcca gcacaccgcc gaccatcagg ccaacaaaca
    25381 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtggaatgt gtcatcatct
    25441 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag atgaaacgag
    25501 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    25561 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    25621 acatgctaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    25681 cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    25741 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    25801 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    25861 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    25921 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    25981 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcttc cgcgcaggaa
    26041 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    26101 agtcagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    26161 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcag
    26221 ttgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    26281 ctgatcccgg tgctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    26341 ttgagcctcg gcagcgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    26401 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcagtagt tggcccgatt
    26461 gcctttattg gcctgatgat gccgcatatg gcgcgttggc tggtgggtgc cgatcatcgc
    26521 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    26581 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    26641 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    26701 gccgattact catcacctgt ttgctgctgg tttccgcctg tgtggttgca ggtatctggg
    26761 gattacgcag cggtgccgtc acgctggaaa cctcgcaggt attcgccgcg ctgatgggcg
    26821 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    26881 cgctgttgat tggcgcagca ctgggcgtca gtggcgcgat ttttcagtcg ctgatgcgta
    26941 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    27001 tggcgatggt gctgtttggt caggacctga cggctatcgc gctgtcagca atggtgggcg
    27061 gcattgtcac ttcgctgctg gtctggctgc tcgcctggcg caacggcatc gacacctttc
    27121 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    27181 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    27241 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    27301 ccgccgcctt actggtacgc cggatgcgct tgctggaaat gggcgatgat accgcgtgtg
    27361 cgctgggcgt cagcgtcgaa cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    27421 ccgctgcggc aacagcgctt gccgggccga tttcctttat tgctttagtc gcaccgcaca
    27481 ttgcccgacg cattagcggc accgctcgct gggggctaac ccagacggcg ctatgcgggg
    27541 cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg tatcaacttc
    27601 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    27661 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    27721 atatggcaaa tataccgttg cggaaaatct gactgtagaa atacctgatg gtcacttcac
    27781 ggcaattatc gggccaaatg gctgcggtaa atccacgtta ctgcgtacct taagccgcct
    27841 gatgacgcct gctcatgggc atgtctggct ggatggcgag cacattcaac attacgccag
    27901 taaagaggtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    27961 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    28021 gcgcaaagag gatgaagaag cggtaacgaa agcaatgcag gccacgggaa taactcatct
    28081 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    28141 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    28201 cagtcatcag attgatttgc tggagttgtt aagcgaactg aaccgcgaaa aaggctatac
    28261 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    28321 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    28381 gattgagcgc atttatggtc tgcgctgcat gatcattgac gatccagttg ccggaacgcc
    28441 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    28501 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaacagca cgccaccaca
    28561 agccaccatc ccgccgatca acgcggaaag gatcacaata atcgccttac ccggaccgtc
    28621 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    28681 gacatgtgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    28741 ttccgcaacg tcagtgaccg ctttttctat ttccagtttg cgttcaatac cgtctgcacc
    28801 gagagaaata gaaaaatcgg ggtcatcttt aacggcctga ccattactgt aaacgggctt
    28861 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    28921 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    28981 gatctccagt ttattacgga cgttttctag cgactctttc accaccaacg tagagatata
    29041 atcgatatac ccgctcaaaa cggtctgcgc ctcttcactg gttggcgcgg taaaacttag
    29101 cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt catcaaccgc
    29161 tttcattttt tcgctcaatg cgacaattgc gcgatgcaaa tccagttcgt cgattttcgc
    29221 ctcttttaat tggtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    29281 cgactgaaac ttcttgataa acaggttaaa tgcttctgta cgatcaattt tgatatccag
    29341 atccagcaca cgaagcttag tgaatgattt ctccaactct tgccactgaa caggttctgg
    29401 aggcgtgaca accgccgcgc tggtccattt ttgcggcagg atgaaagaga tcagcaagcc
    29461 tgcgcaggca aacgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaactga
    29521 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat aatcgggaaa
    29581 atgagcgtca cttccctgtt taatattcag tgatgacatg gggtataacc tgaaaatgag
    29641 tccaataggg ggaaatctcc acggcgctca ctttaccagt tggtgataat tatccgaagc
    29701 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    29761 tcaaacctat aaatcaaaca aataaaaaac ccataattat tttctgtaat tatgggttta
    29821 taaataatat taatttacct gtttagcgtt gcgcgaataa tcggcccaat tttttcaaac
    29881 gtccctggag agataatatc cacatgcgca caatcctgac gatagatatc cagctccgct
    29941 atccacggcg accaggcgcg ttcgggactc ataccttcct gaagtgtgcg ttcagcaaca
    30001 aacagcgtcg ctttaccgtc aaacggtacg ctatgagcag tcgtcagcag gcgcacagca
    30061 tcagcgtagt tgccttcaat ggtggtaaac aactccgttg aagtacttcc ctgctgtgct
    30121 gccaggaagg cctcgcgttc gcggttaatc tccgccagca cttccgggtc cagaccatta
    30181 gcttcttttt cctgccagtt ttgcgtttct ggcggccagg tatccagcaa gccaagaaat
    30241 gccacctgtt cgccacgggc acgcagtcgc gccgcaatac cctgcgccag cgtaccgcca
    30301 agggaatacc ccagcaggta ataagggcca tgcggttgtt gttcaagtaa cgttgccaga
    30361 tgcgcttcgc agacttcatc caggtttgcc gccgtctgca tggggccatt ggggcgcggt
    30421 gactgaatgc cgataatcga ccattgtgga tcgagataac gcgagagcac gctgaactgc
    30481 caggcaaaac cggacgcagg atggaaacaa aacagcgtcg ggccattacc ttcacgcaac
    30541 ggcagaatgg tttcgaatcc catacgccgg gtgctgtctt cttcagcatc aataatcgtt
    30601 gccagtttgg cgacagttga cgcgaccatc acttgccccg gcgtcacctg gcgggcaacc
    30661 tgccgactta actgcgctgc cagtttcatt gccagtagcg aatgaccgcc aagcgcgaag
    30721 aaatcagcat cggcatcctg cacgtcacac cccagcaacg acgagaatgc cgcggcgata
    30781 atcgtttcac tgcccgcttt cggcgcacgc cctggcgctt gtgccttcag ttcaggcaac
    30841 ggtaaggctt tgcgatccag cttgccgttg gcgctaagtg gtaactgtgg aagttgcagc
    30901 agaaccaccg gtaccatatg tggtggcaat gtttcacgaa gctgcgcctg caatgcgctg
    30961 gtatccaacg gcaggcccga ttgcgacacc agatagccca ccaattgacg cgcatcacca
    31021 ccggtggcag ccgcctggtt aatcacacag gcgtgggtaa cggcttgttc gacatccggc
    31081 agcgcctgca tcacgcgatc gatttcgccc agttcgatac gctgcccgcg aatttttagc
    31141 tgatcatcac tgcgcccgag gtactccacc gcgccgttat ccagccagcg ggcaacgtct
    31201 ccggtacggt acatccgttc acctggggca aaaggatcgg caataaagcg gctggcggtc
    31261 agatcggggc gtccgagata gccctgcgcc agttgaatgc cagtgagata gagatcaccc
    31321 gccacacccg gcggcaccgg atgcatcatc gcatcaagaa tacgcagacc cgtattccat
    31381 accggataac caatcggcac actgctgccg cgcacctgtg ccagttcctc gccaaaagcc
    31441 ggataccagc tgacatctac cgccgcttcc gtcgggccat atagattatg caacggcgcg
    31501 ccagttaact gttgccattc gcggcataaa tcagccggta aggcctcacc actacagaaa
    31561 acctgtttca acgtcgcgca actctggcga gcggtttgcg gcgtcagcga ggcaacaaat
    31621 gccgccagca tcgacggcac aaagtgcgtg gtcgttacgc catattcggc aaagaattgc
    31681 tgcatagcga gcgggtcgcg gtgcgcttcc ggttcagcca tcaccagttt tgcccctgcg
    31741 ataaacggcc agaaaaactc ccacaccgag acatcaaaac tgcacggcgt tttttgggca
    31801 acgacatctt cgcctgtaag cggataatga ttttgcatcc aaagcaggcg gttgacgata
    31861 gccgtctgcc cgaccattac ccctttcggc ctgccggtgg agccagaggt aaagatgata
    31921 taagccgtgt gatgcggttg tgaaagttgc agcggcgcac tgccctgcgg tgtaagcggg
    31981 gcgttatagc aaaggcttgt taaattggga acatcgctaa agcgcggcag ttgatcgtcg
    32041 gtggtaatta acagcgacgg acgcgcatct tccagcatca ttttcaggcg atcgtccgga
    32101 tagccggtat ccagcggtag ccaggccgct ccagcttcaa ctatcgcatg gagtgccagg
    32161 gtcaaaaaga ccgagcgcgg tagtgccacc gccacgctgt cccctggttt aacgccgcgc
    32221 tcacgcagca gattcgccag cgccaccacc tgctcgcgca tttcccgata gctgaacagg
    32281 taacgcgcat ctgccagcgc cggagcatcc ggtgtttttg ccgcttgttc tgccaccagc
    32341 gcgctaagcg tggtttctgg aatctcaacc tgagtggcgt tgagctgcgc cagctgcgca
    32401 tactcacctg gcagcataat atcgacatcg ccgcacaaca gcgccggatc cgcggcgaac
    32461 tgggcaatca gcattttcag gcgttcagca tgctggatta acgttggctc atcgtaacgc
    32521 tgtttattgg cgaggatctc aatactcaaa tcaccgtgtt catccgggaa cagggccagt
    32581 tcaaggtcat taaccggacc ggttgccagg gtatgggttt gcgcctgaac accaggaata
    32641 tccagttggt aatcaaatac cttgatattg agtaccggac caaacagcgg ttcatcacct
    32701 gccgctcgcc cgctgtcacg gacaatttgt tcggcatcgt aacgttgatg acgacgcatt
    32761 tttttcagtt gagccgccag tcgggttgcc agttccggca gcgtttcttg cgccgcaata
    32821 tgaataccca acggcaaaac gttgagcacg ggtccggtag ccgtcaacgc cgccgagccc
    32881 agtcgacgca taaagataaa tccggcagcg tagtccatgc ggttgcacaa tcgccccagc
    32941 cacaaagccg ccagcgcaag ggctaaatcg gtacgctgca cacctgaaag ttgcatagcc
    33001 agctggcgga attccccgtc ggtaaattcc agtttcaggc gcagaatatc tgccgaggcg
    33061 ctgcgccccg gtaaaggtgc cggagaaagt gacgcgggcg gtggcagttg acgacgctgt
    33121 tccgcccaga atgccgcatc acgctgccag gcttcgcttt cgcggtattg ctggtactct
    33181 tccactacat cagcgaaagg cgtaaacggc gaagcaggcg ttggttcgcc acgcagccat
    33241 gtgcagtaaa tattggcgat ctggcgggta atggccggga aactaaagcc atcgaccagc
    33301 aaatggtgat aacgctgata ccagtaccag cggttatctg ccacctgaat gagctgatga
    33361 aagaccagtg gtttaccgct atcgacgcgc agatcttgct gcaaatcggc ctgcattaat
    33421 gcctgcgcag taccgtgcgg atcaatgttg gttcgcaggt cgataatttc tggcagttcg
    33481 aacgtcagcg catcatcgac ccactgccag acttcgccgt tatcttccgt aaaacgcatc
    33541 cgcagcgtat ctgcttgcgc tagtccggca accaccgcgc gggccagtaa tggcgaatca
    33601 acctctccgg ttaactcaac gtaatgcgcc acgctccagg cggagggtaa ttctgacagt
    33661 ttttctgcca tccagatgcc gggctgtgcg gcgaccaaag gtaaatgctg gctcattgtg
    33721 cctcctgcaa ctgggtaaaa ttcgtcggtg tcagagtacg ccagtgggct tccagccact
    33781 gctggcagga cgcctgtgac tgcggctgac acacaatgtc ccagcctgcc ggtaagacgc
    33841 attgttgcgg ccacagactg aattgcccct gcgcattgcg caatatgtaa aacgctccct
    33901 gcggatcatc gaagggatta ctgaatgcca tattcaactc ctgtcatgga aaagtggttg
    33961 ccagaggtcg attagcccct gcatcaagcc accgcgccaa caaagcgcat catgtccgcc
    34021 gtcaacctga cgccagaaaa tggattcttt tatggggtgt aattgcgcat acagcgcctg
    34081 attggcccgc atgatcatcg gctcgcgaat acccgcttcc agcacaatgc gcagaccttc
    34141 ggcgctaact tcaccagctt ttagcttttc aagtaacacg ccctcttgct gcccgccccg
    34201 atgcggccac cagtacgatc ctgactggct taatacacag ccaaagcgtt caggccagtg
    34261 cagtccggca tacagcgcgg aaagcccacc aaaactctgc ccggcaacca cggtgcgatc
    34321 ggcacgatcg ctaaaagggg caatagcttt caccaggggt aataactctt gctgtactgc
    34381 gagccagaaa tccgcattac acggcagttc gtgggcgcgg tgcgtggtgt cgatagcgtc
    34441 gatcaacaca tacacggcgg gaggaagttg ctgacgatgg gtcagcgaag tcagcactgg
    34501 ccagacgggc atactttgcg cccaaaattc gccatcgagc aaaactgcca gcgggcgttc
    34561 ttctgctgtt gcatcgccgg tggtaaaaat ccatacacgc cgtgactttt tcaaccgttc
    34621 acttttccag ataatttctt tggcaggtat ttctggcgct tgcggacaat cccatcccgg
    34681 ttgcagaggc gcttgcggca tttcgagtgc agaaacagcg tgccctcgcc cgcctttcca
    34741 gctttgtagg ttcagcggat cggctatcgc ctggggtaat agttttcgcc agccttcgcg
    34801 caattcgagg cgatcggggg atggtacaga aaaaatgtca tcgcgttcgg tgggaataaa
    34861 gcagtagctg ccgcgccagt tggcattgag ttgtgtcttc cactgccaga cgttagtgcc
    34921 tgcaattcgc tgcatcgact ggggctggct gttctgatgg tgatcggtca caccagtgat
    34981 gtagacccat acgcgcttta tcgtcgagta ttcttcagaa ccttggggat cacgccacca
    35041 gaaagtgacc tcaaacattt cgtcattcag acgctgccat tccgggccat gtttcgactg
    35101 ccaccagctc tcacttccta cttttaacgc cgtcacacca taaccccatg tttactgtgc
    35161 aatttttcat tgattgcaga aatatattga taatattatt gataactatt tgcatttgca
    35221 atagcgtaat ggcgcgccgt gggaagcgcg gacattaatt aaccaactgc actgcgtgtc
    35281 tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt gttgatgacg accatgcccg
    35341 acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat gaacaagaag attcattccc
    35401 tggccttgtt ggtcaatctg gggatttatg gggtagcgca ggcacaagag ccgaccgata
    35461 ctcctgtttc acatgacgat actattgtcg ttaccgccgc cgagcagaac ttacaggcgc
    35521 ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa cccggttgcc cgcgatgtgt
    35581 cggagatcat ccgtaccatg ccaggcgtta acctgaccgg taactccacc agtggtcagc
    35641 gtgggaataa ccgacagatt gatattcgcg gtatgggtcc ggaaaacacg ctgattttga
    35701 ttgacggcaa gccggtaagc agccgtaact cggtgcgtca gggctggcgt ggcgagcgcg
    35761 atacccgtgg tgatacttcc tgggtgccac ctgaaatgat tgaacgtatt gaagttctgc
    35821 gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg cggcgtggtt aacatcatta
    35881 ccaaaaaagg cagcggcgag tggcacggct cctgggacgc atatttcaat gcgccagaac
    35941 ataaagagga aggtgccacc aaacgcacta actttagcct gaccggtccg ctgggcgacg
    36001 aattcagctt ccgtttgtat ggcaacctcg acaaaaccca ggctgacgcg tgggatatca
    36061 accagggcca tcagtccgcg cgtgccggaa cgtatgccac gacgttacca gccgggcgcg
    36121 aaggggtaat caacaaagat attaatggcg tggtgcgctg ggatttcgcg ccattgcaat
    36181 cgctggaact ggaagcaggt tacagccgcc agggtaacct gtatgcgggc gacacccaga
    36241 ataccaactc cgattcctat acccgctcga aatatggcga tgaaaccaac cgtctgtatc
    36301 gccagaacta cgcgctgacc tggaacggtg gctgggataa cggcgtgacc accagcaact
    36361 gggtgcagta cgaacacacc cgtaactcgc gtattccgga aggtctggcg ggcggtaccg
    36421 aagggaaatt taacgaaaaa gcgacacagg atttcgtcga tatcgatctt gatgacgtga
    36481 tgctgcatag cgaagttaac ctgccgattg atttcctcgt taaccagacg ctgacgctgg
    36541 gtacggagtg gaatcagcaa cggatgaagg acttaagttc caacacccag gcactgaccg
    36601 gaacgaatac cggtggcgct attgatggcg tgagtgccac cgaccgtagc ccgtattcaa
    36661 aagcagaaat tttctcgctg tttgccgaaa acaacatgga gctgactgac agcaccatcg
    36721 taacgccggg gctgcgtttc gatcatcaca gtattgtcgg caataactgg agcccggcgc
    36781 tgaacatatc gcaaggttta ggcgatgact tcacgctgaa aatgggcatc gcccgtgctt
    36841 ataaagcgcc gagcctgtac cagactaacc cgaactacat tctctacagt aaaggtcagg
    36901 gttgctatgc cagcgcgggc ggctgctatc tgcaaggtaa cgatgacctg aaagcagaaa
    36961 ccagcatcaa caaagagatt ggtctggagt tcaaacgcga cggctggctg gcgggcgtca
    37021 cctggttccg taacgattat cgcaataaga ttgaagcggg ctatgtggct gtagggcaaa
    37081 acgcagtcgg caccgatctc tatcagtggg ataacgtgcc gaaagcggtg gttgaaggtc
    37141 tggaaggatc gttaaacgta ccggttagcg aaacggtgat gtggaccaat aacatcactt
    37201 atatgctgaa gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc ccggagtata
    37261 cgttgaactc aacgctgagc tggcaggcac gggaagattt gtcgatgcaa acgaccttca
    37321 cctggtacgg caagcagcag ccgaagaagt acaactataa aggtcagcca gcggttggac
    37381 cggaaaccaa agaaattagt ccttacagca ttgttggcct gagcgcgacc tgggatgtga
    37441 cgaagaatgt cagtctgacc ggcggcgtgg acaatctgtt cgacaaacgt ttgtggcgtg
    37501 cgggtaatgc ccagaccacg ggcgatttgg caggggccaa ctatatcgcc ggtgccgggg
    37561 cgtataccta taacgagccg ggacgtacgt ggtatatgag cgtaaacact cacttctgat
    37621 gctaacgtca gattgttgac aaagtgcgcg tcgttcatgc cggatgcggc gtgaacgcct
    37681 tatccggcct acaaaaatct tgccaattca atatattgca ggaccatgta ggcctgataa
    37741 gcgtagcgca tcaggcagtt ttgcgtttgt catcagtctc gaatatggtc gatatgaaaa
    37801 ctacgcatac ctccctcccc tttgccggac atacgctgca ttttgttgag ttcgatccgg
    37861 cgaatttttg tgagcaggat ttactctggc tgccgcacta cgcacaactg caacacgctg
    37921 gacgtaaacg taaaacagag catttagccg gacggatcgc tgctgtttat gctttgcggg
    37981 aatatggcta taaatgtgtg cccgcaatcg gcgagctacg ccaaccggtg tggcctgcgg
    38041 aggtatacgg cagtattagc cactgcgggg ctacggcatt ggccgtggtt tcccgtcaac
    38101 cgattggcgt tgatatcgaa gaaatttttt ctgcgcaaac agcgaccgaa ttgacagaca
    38161 acattattac accagcagaa cacgagcgac tcgcagactg cggtttagcc ttttctctgg
    38221 cgctgacact ggcattttcc gccaaagaga gcgcatttaa ggcaagtgag atccaaactg
    38281 atgcaggttt tctggactat cagataatta gctggaataa acagcaggtc atcattcatc
    38341 gtgagaatga gatgtttgct gtgcactggc agataaaaga aaagatagtc ataacgctgt
    38401 gccaacacga ttaattgaca acatctggta cgattcgccc gcagccatca ctgaccacgg
    38461 gcgaaagtgt aaagcaggtg ccttaccatc ctgacctgac aaccggatat gcggggatt
//
LOCUS       DACAPB010000021        37932 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.021, whole genome shotgun sequence.
ACCESSION   DACAPB010000021 DACAPB010000000
VERSION     DACAPB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.021"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(79..1596)
                     /gene="lysS"
                     /locus_tag="IT904_000969"
     CDS             complement(79..1596)
                     /gene="lysS"
                     /locus_tag="IT904_000969"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP3190794.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1833..3290)
                     /gene="dtpC"
                     /locus_tag="IT904_000970"
     CDS             complement(1833..3290)
                     /gene="dtpC"
                     /locus_tag="IT904_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HAP3190795.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3349..5496)
                     /gene="cadA"
                     /locus_tag="IT904_000971"
     CDS             complement(3349..5496)
                     /gene="cadA"
                     /locus_tag="IT904_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HAP3190796.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5576..6910)
                     /gene="cadB"
                     /locus_tag="IT904_000972"
     CDS             complement(5576..6910)
                     /gene="cadB"
                     /locus_tag="IT904_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAP3190797.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7275..8813)
                     /gene="cadC"
                     /locus_tag="IT904_000973"
                     /pseudo
     CDS             complement(7275..8813)
                     /gene="cadC"
                     /locus_tag="IT904_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
     gene            complement(9430..9505)
                     /locus_tag="IT904_000974"
     tRNA            complement(9430..9505)
                     /locus_tag="IT904_000974"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9470..9472),aa:Phe,seq:gaa)
     gene            complement(9612..10187)
                     /locus_tag="IT904_000975"
     CDS             complement(9612..10187)
                     /locus_tag="IT904_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP3190798.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(10224..11921)
                     /locus_tag="IT904_000976"
     CDS             complement(10224..11921)
                     /locus_tag="IT904_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAP3190799.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRIVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(11897..12235)
                     /gene="cutA"
                     /locus_tag="IT904_000977"
     CDS             complement(11897..12235)
                     /gene="cutA"
                     /locus_tag="IT904_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAP3190800.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(12351..13652)
                     /locus_tag="IT904_000978"
     CDS             complement(12351..13652)
                     /locus_tag="IT904_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP3190801.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(13770..15206)
                     /gene="aspA"
                     /locus_tag="IT904_000979"
     CDS             complement(13770..15206)
                     /gene="aspA"
                     /locus_tag="IT904_000979"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAP3190802.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            15543..16019
                     /gene="fxsA"
                     /locus_tag="IT904_000980"
     CDS             15543..16019
                     /gene="fxsA"
                     /locus_tag="IT904_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAP3190803.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(16035..17291)
                     /gene="yjeH"
                     /locus_tag="IT904_000981"
     CDS             complement(16035..17291)
                     /gene="yjeH"
                     /locus_tag="IT904_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAP3190804.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            17567..17860
                     /locus_tag="IT904_000982"
     CDS             17567..17860
                     /locus_tag="IT904_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP3190805.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            17904..19550
                     /gene="groL"
                     /locus_tag="IT904_000983"
     CDS             17904..19550
                     /gene="groL"
                     /locus_tag="IT904_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP3190806.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            19688..20041
                     /locus_tag="IT904_000984"
     CDS             19688..20041
                     /locus_tag="IT904_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAP3190807.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(20244..21113)
                     /locus_tag="IT904_000985"
     CDS             complement(20244..21113)
                     /locus_tag="IT904_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190808.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIETITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(21508..22536)
                     /gene="epmB"
                     /locus_tag="IT904_000986"
     CDS             complement(21508..22536)
                     /gene="epmB"
                     /locus_tag="IT904_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAP3190809.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            22578..23144
                     /gene="efp"
                     /locus_tag="IT904_000987"
     CDS             22578..23144
                     /gene="efp"
                     /locus_tag="IT904_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP3190810.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            23196..23321
                     /gene="ecnA"
                     /locus_tag="IT904_000988"
     CDS             23196..23321
                     /gene="ecnA"
                     /locus_tag="IT904_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAP3190811.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            23432..23578
                     /gene="ecnB"
                     /locus_tag="IT904_000989"
     CDS             23432..23578
                     /gene="ecnB"
                     /locus_tag="IT904_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAP3190812.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            23760..24077
                     /locus_tag="IT904_000990"
     CDS             23760..24077
                     /locus_tag="IT904_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP3190813.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(24074..24607)
                     /locus_tag="IT904_000991"
     CDS             complement(24074..24607)
                     /locus_tag="IT904_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAP3190814.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(24696..25829)
                     /gene="blaEC"
                     /locus_tag="IT904_000992"
     CDS             complement(24696..25829)
                     /gene="blaEC"
                     /locus_tag="IT904_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP3190815.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            complement(25892..26251)
                     /gene="frdD"
                     /locus_tag="IT904_000993"
     CDS             complement(25892..26251)
                     /gene="frdD"
                     /locus_tag="IT904_000993"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP3190816.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLWL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(26262..26657)
                     /gene="frdC"
                     /locus_tag="IT904_000994"
     CDS             complement(26262..26657)
                     /gene="frdC"
                     /locus_tag="IT904_000994"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP3190817.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(26668..27402)
                     /locus_tag="IT904_000995"
     CDS             complement(26668..27402)
                     /locus_tag="IT904_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP3190818.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(27395..29203)
                     /gene="frdA"
                     /locus_tag="IT904_000996"
     CDS             complement(27395..29203)
                     /gene="frdA"
                     /locus_tag="IT904_000996"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP3190819.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29528..30505
                     /gene="epmA"
                     /locus_tag="IT904_000997"
     CDS             29528..30505
                     /gene="epmA"
                     /locus_tag="IT904_000997"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP3190820.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            30724..32226
                     /gene="yjeM"
                     /locus_tag="IT904_000998"
     CDS             30724..32226
                     /gene="yjeM"
                     /locus_tag="IT904_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP3190821.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            32278..32592
                     /locus_tag="IT904_000999"
     CDS             32278..32592
                     /locus_tag="IT904_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190822.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            32589..32903
                     /locus_tag="IT904_001000"
     CDS             32589..32903
                     /locus_tag="IT904_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HAP3190823.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(32932..36255)
                     /gene="mscM"
                     /locus_tag="IT904_001001"
     CDS             complement(32932..36255)
                     /gene="mscM"
                     /locus_tag="IT904_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP3190824.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(36277..37245)
                     /gene="psd"
                     /locus_tag="IT904_001002"
     CDS             complement(36277..37245)
                     /gene="psd"
                     /locus_tag="IT904_001002"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAP3190825.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(37342..>37932)
                     /gene="rsgA"
                     /locus_tag="IT904_001003"
     CDS             complement(37342..>37932)
                     /gene="rsgA"
                     /locus_tag="IT904_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP3190826.1"
                     /translation="PIIVLNKIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGL
                     KPLEEALTGRISIFAGQSGVGKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLY
                     HFPHGGDVIDSPGVREFGLWHLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIRE
                     AVEEGKIAETRFENYHRILESMAQVKTRKNFSDTDD"
BASE COUNT         9484 a         9408 c         9568 g         9472 t
ORIGIN      
        1 agcgtctctc ctcaaaactg gatttgcgga acgatgaacg aagcagtcgc ctgactgctt
       61 cgttcattaa agtgaaattt atttctgtgg gcgcatcgcc gggaagagaa taacgtcacg
      121 aatagtatga ctgtttgtaa acagcataat cattcggtcg atgccaatac ccagaccagc
      181 ggttggcggc agaccatatt ccagcgcagt cacgtaatct tcgtcataga acatggcttc
      241 gtcgtcacct gcagctttag cgttaacctg ttcctggaaa cgttcagcct gatcttctgc
      301 gtcgtttaat tcgctaaaac cattaccgat ttcacgacca ccgatgaaga attcaaaacg
      361 gtcggtgatt tccgggttaa catcattacg gcgcgccagc ggggacactt ctgccggata
      421 ttccgtaata aaggttggct gaatcagatg tgcttctgcc acttcatcaa agatctccgt
      481 gacaatacgt cccagccccc agcttttctc taccgtaatt ccgatagatt cagctaatgc
      541 tttagcagca tcaaaattat ccaggtcggc catatcggtt tctggacgat attttttgat
      601 tgcttcgcgc atggtgagtt tttcaaacgg tttgccgaaa tcaaacacat gctcgccata
      661 agtgacttta gtggtaccca gaacctcttg tgccagggtg cggaacagtg actctgtcag
      721 ttcaatcaaa tcgtggtaat ccgcatacgc catgtagagt tccatcattg tgaactcagg
      781 attatggcga acagaaatac cttcattacg gaagttacgg ttgatttcga atacccgttc
      841 aaaaccgcct acaaccagac gtttcagata cagctccggc gcgatacgca gatacatatc
      901 taaatccaga gcattatgat gggtaataaa cgggcgagca gatgccccac ctggaattac
      961 ctgcatcatc ggggtttcta cttccataaa gccgcgcgcg accatgaact gacggatagc
     1021 ggccagaatt tttgaacgga caacaaacgt ttgacgggat ttatcgttag cgatgaggtc
     1081 cagataacgt tgacgataac ggacttcctg atcctgcaga ccatggaatt tatctggtaa
     1141 aggacgtagt gcttttgtca gcaggcgcaa ctcagtacag tgaatggaaa gctcacccgt
     1201 ttgcgtctta aacagcgtac cgcgggcacc gataatgtca cccagatccc attttttaaa
     1261 ctgatcgtta taaacacctt ctggcaggct atctcttgca acgtacagtt gaatacggcc
     1321 accgacatcc tgcaacgtta caaaggaggc tttccccatg atacgacggg tcatcattcg
     1381 gccagcaacc gagacttcaa tgtttaagga ttccagttcc tggttatcct tcgcatcaaa
     1441 ctcttcgtgc aactggtcag aggtatggtc gcggcgaaaa tcattgggaa acgccacacc
     1501 ttgctgacgt agtgccgcca gtttttcgcg gcgatttctc agttcatcgt taaaatcaat
     1561 agcctcattg gctccccgtg tttcttgttc agacattttg gttcctctaa atccagcttt
     1621 caatttagcg tagataaaga gacagatcgg tacactgtat tatctgcctc gactattagt
     1681 aactcagttt atcttatcga aactaacgct tacctcaagt tgatatcact aataaataac
     1741 catcaaaatg gtttaaatga cttattaatc tcgatttgtt agtgttataa actgaaagtt
     1801 aatttattca taaccaattg tttttacgac agttaatcgt tgctctcctg tatcatattc
     1861 gtcggcgtgc taaacagaaa acgggtagca aaggccaata cgacgatgat cgcgacacaa
     1921 gccaacgtcc actctcccat ctgagaaaag aatcgctggt aagctgcaat tgccataccg
     1981 ctaatttgcg actctgtcgt ctgctgtgcc acgacgcctg ccagccagtt ggcgaccgcg
     2041 cctgttgcca gcatataaat cccggttaat acgccagaca ttttcagacg cgtgatttgc
     2101 gcaatcgcca ccgggtcaat aaagagttcg gcaaagccca ttagcgccag cccggatatc
     2161 atcacgccca ttgacgcttg accgtcagcc gctgcatgtc gggcatcaaa tgccaacaac
     2221 ataaagccac aagccatcag cagtaagcca aaggcaaact tcagccagac gcgcaatgtt
     2281 gagttgccgc ggctttctgg cgacgccagc caggccagta caaccccagc gagcatcacc
     2341 gcaattgcat tcaccgactg gaatagtgct gtaggtactt caatattgaa tgcctgacga
     2401 ttcacaaagc gatcgataaa caagctgatg gtactgccgc cctgttgtgc cagtacccag
     2461 aacaatgtcc cgacaaacat caacaataca atttgccaaa gagcacggcg atgttcgggg
     2521 aatttgatca tcatgcgggc aatgatttgt gcggcaatga ggcaaacgat cgccagcaaa
     2581 tatccggacc agtcgttctc cagcagcaga gtaaaaaata ctggggctaa acagagcatc
     2641 accactaacc agctccatac tggtaaggca aatttgacac tagtgagcgc ttttttatcc
     2701 atactacgtg tggactggaa atgacgatga ccgcttaaga aaatcaacaa accgataaac
     2761 atgccgccac ccgcaagggc aaagccaaca tgccatccat accactgagc agccaggccg
     2821 caggcgattg gggctgcgat agaaccgata ttgcccgcag catacagcag cgaaaaaccg
     2881 ccatcacgtc gatgatcgtt ctcgtcgtag agctcgccaa gcaaacagct gatgtttgat
     2941 ttgaataaac cgtagccaca aataatgatt gccagcgcca gatacaggct aaaggttgaa
     3001 tttgtatcaa tacccagcac cacatggcca agggtcatta acagcgcgcc ggcaatcact
     3061 gcagtgcggt tgccgagcag gcggtcggca agccagccgc cgagaatagg ggtaacgtaa
     3121 accagagaag catatgcgct gaacaggctg atggcatggt tatcatcaaa accaagctga
     3181 tgggtgagat agagaatgag taaggcacgc atgccgtaaa aactgaagta ctcccagatt
     3241 tggatcgcca cgatatagta tatcgcgcgc ggctgtgagg gtgttttcat gtgttctcct
     3301 tatgagcaaa aaagggaagt ggcaagccac ttcccttgta cgagctaatt attttttgct
     3361 ttcttctttc aataccttaa cggtatagcg gccatcagcc tgacggtatg caccgtgaat
     3421 atcggtttca aagcccggat agtgagcgcc gatttcacac agcatctgta ggaactccag
     3481 aaccggacgg ctttcttcgg tgatcatttc acccggcatt accagaggaa ctcccggcgg
     3541 gtacggaagg atcatattgg cgttaatacg acctaccatt tcgtcgaggt aaacttcttc
     3601 ggtcataccg tgcagctctt tctggaatgc agcatacgga gtcattacca tcgtcggcag
     3661 cacttcaaat gcgcgataca tcagatccgg cagattgtgg tgaacaatca gtttgtggat
     3721 attctgagcc agttcctgaa tacgcatgtt ttcatagaat tcaggatctt cacgatacag
     3781 agacggcagc atgtttttca cacgcaggtt caggtcgaac gcacgtttaa agtcagtcag
     3841 agcacgcagc aggctcagtg ctttggtctt atcgataccg atgctgaaca ggaacagcag
     3901 gttatacgga ccggttttct caacaacgat gccatgttcg tcgaggtatt tcgccacgat
     3961 gctggccgga ataccaaagt cgctcatggt gccgtctttt tccatccccg gagtcagcag
     4021 ggtgactttg atcgggtcaa gatacatgtg ctcgttatcg atgtttttga agccgtgcca
     4081 ggtgctgtca gaacgcagcg gccagcattc agtcgtatcg atatgatccg gctgccatac
     4141 atcaaagaac cagccatcag attccgttct cagacgtttg atctctttac ggaatttgat
     4201 cgcacgttca atggaaccgt tgatcagacg cttacctgca ttgcctttca tcatcgccgc
     4261 agcggtttca gtggacgcca cgataccgta gtgcggagaa gtggtggtgt gcatcatgta
     4321 ggcttcgtta aaggtttctt cgtttacgtc acctttaacg tggatcatgg aagcctgaga
     4381 gaacgccgcc agcagtttgt gagtggactg ggtttcgtaa atcactttcc cttctacacg
     4441 gccaccgctc ataccgcatt taccttcgta aatcggtgag aagttggtgt aaggcaccca
     4501 cgcggagtca aagtggatgg atttcacatc cagtgttttc ttgatgaagt cggtgttgta
     4561 cagcagacca tcataggtag agttggtaat tacagcatgt accggccagg ttgcgtttgg
     4621 tgtttctttc acgcgcttag caatggtagc gtgctggaat tcactctgtg ggataccacc
     4681 aagaataccg taagcgttac gggtcgggcg gaaatagatt ggcgtaacat cgctcatcat
     4741 catcaggtgg gtcagcgatt tgtggcagtt acggtcaatc agaatggtgc tgcctgctgg
     4801 agcagagtac ataccaacaa ttttgttcgc agtggaagta ccgttggtca ccatgtagct
     4861 gcggtctgcg ttaaagacgc gagcgatata ctgttctgct tctttgtgtg gaccactgtg
     4921 atccagcaga gaacccagtt cagatactga aatggaaata tcagatttca tggtattcgg
     4981 accaaagaaa tcatagaaca ggctacctac cgggcttttc tggaatgcag taccgcccat
     5041 gtgaccagga gtacagaaag tatatttacc ttcacgaaca tatttaaaca gtgctttagt
     5101 cagcggaggc agaatagtgt tgatatattc gtcagtggtc tgcttgatct tattagcaat
     5161 atcttcagca gcacccagcg catattcaaa gaagctaatc tgtaaacgca ggtcattcag
     5221 gcttacatcg agagtggaat acgtattagc gaacgcgtac aacggcaggt tctcgttcat
     5281 tttgctaatt tcttcgcaca gctcgagatt atatttatcc cagtcaaaaa taacgccgca
     5341 cagacgcgca ttgttttcga tcagttttaa taagtcgtca cggtcgttcg ggtaaacaat
     5401 ctggaagttc agacgttcaa gcgcgcgatg aagttcacgg atgggttctt ctttaaaata
     5461 aacccccatg tgattcaata ttgcaataac gttcatagtc atatctccag gtaaaaaagg
     5521 cccctcccaa cacatgggac aaaatgaaag gaggagcctc ggaaaatact tttaattaat
     5581 gtgcgttaga cgcggtgtgg ttatccattg agtggctctg gcgctcgtgc attttgcgag
     5641 cgtagaacat caggataatc aggctgacga tgaaggtacc tgccagctcg aaggagcttg
     5701 cgcccatcag cgcgatgaag cagaacacgc aacccagtac agagcagatc aggctgacaa
     5761 agttgcggat gttaacgcct tcaaaacgaa tcaggtcaac gcaagagtag aaatacggca
     5821 gcatagtcag cagtactgcg ataccggtca gttcaccgaa caggtcagat gctttaccac
     5881 cggcagagtt catcagagtg ataaggatca tcagggcagt cattttcact gcagccagca
     5941 gcagaccttt tttcggaata ccgttgctgt cgacttcacc ataaactttc gggaagttac
     6001 cgtcgttagc ggcacgtaca cctgcctggc ctaccaacat catccaggag cccagagaag
     6061 tcaggcacgc aaaggcggtg aatgcagaaa ccagtggcgc agcccagtta ccgaggatag
     6121 ttgaagcact gattgcaaac ggagcaccgg aagccgccat tacagaagac ggatacatac
     6181 cggaaagcac ctgagtcgca gcgatgtaaa caatacctgc taaaccagta cccagcatgg
     6241 ttgccagcgg aacggtacgt ttcgggtttt taaccatacc agtacttaca gctgcggatt
     6301 caacacccac gaaggcccac aggcagagca gaatactttt aatgatcgca tgaccatcag
     6361 tggtatccgc agtattccag ttagctgcat aagttgccgc atcaaaccaa tgccagccaa
     6421 caatagcagt catcaccaca ggaataagaa ccagcaccag accaatagtg gttaaacggc
     6481 ttacccaagt accgccgagc atatttacaa aggtaaatac ccagacgata gcaatacagg
     6541 cgatacccgc cggaacagga tcatttaata ctgggaagaa ggtggaaaga taagatacag
     6601 cggtaatacc aatcgccagg ttaccaatcc agttagcatg gtaataaaga acacctgtct
     6661 gaaaaccaaa tgcaggggaa atttctccgg cataagcaat tgggccacct tgttgcgggt
     6721 tttttgttgc cagtcgggca tatacatacg ccagcgacat tgcaccaata atagagataa
     6781 tccaacccca gatagcaata ccaccgatac ttgctaggtt cgcaggtaat aatgcaatac
     6841 cgctccccat catattaccg gcaacaacac cggtacaggc aaatagcccg atcttcttgg
     6901 cagaactcat gctcttctcc taatttcatt tttgaatttg gagtccgggt catgatgtat
     6961 aactatttcc tgaccagacc aaactggcga taagattact cacgaaaaaa ggattaatcc
     7021 taaagattag gtgaaataac acaaaagttt ctgtaagtga gaacttgagg ttttttatta
     7081 acacatcagg atcgcaagtt gatatcatga aaagataaac atttaatgtt tacaatgaat
     7141 tgcgtgacat tctctggtta aatttatgta ataaaaatta tgcggcaaat aaattgccgc
     7201 aacatattat accaacagga acatacaaaa actcaacaac aaatatttcc gagcataaat
     7261 caacccggag ttacttattc tgaagcaaga aatttgtcga gataaggtac aacataagga
     7321 acagaagtct ggaatatacc attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa
     7381 ttaaaggcgg tgagatatgc atcagctgct tcccggttca tccccttcat ttaataaacc
     7441 ttgccaagca acacataatt tagccaggac atttcaagat caatgccagt atttatcgcc
     7501 tggtaagact catctgtttt accttttacc agagcactga ccgcttttat ttgatatata
     7561 atggacaggt tgttcaattc cggcagtgta acaatgttat ctatttctgt gttcagtgct
     7621 gctaattgtt tttcatctaa aggatgttga gaatggcgca cgatatcaac taatgctttt
     7681 tctgctctcg cgtaggtaaa ttctggggat gattgaacaa tctcacctaa taattcactg
     7741 gcacggttca atgatttatc atcgccatgc agtaaataat catgtgcctg ataaaaatta
     7801 gttaataacg caccacgatg cggcaaaatt ttctggagcg tctcctgcat tcgttgtggc
     7861 cacggttggt ttaacgcttt tgataaactc tccagtaaat cattttgaat cgccagctga
     7921 ttaccgttag tgatgacata acgtttatcc agcatggttg aaccatctgc attgtctacc
     7981 aattttatcg acataaagca ttgttgagca cggtattggc gctgattaac aaacgcaata
     8041 gataatgttt taccggaact gctcggttca tcaatgttgt agttgatttt gtcatgcacc
     8101 ataaaggtgg agaaggtgtt aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa
     8161 gagagctgat acggggaact ccagctgtta caacttttat ttaccatatt aatgtcaata
     8221 tcgcgtggat tgagcaaaat acgcgatttg ctcataggaa gacgtgtatc aagacttgaa
     8281 aacgctacca gtgctacaca gatacctaac gacaacagga aaaaaaacca tacccaaaag
     8341 gtagtgaatc gtttgctttt aactggggat tgttcaggtg gcgttgcggt gttttgaatg
     8401 ttaagactgt gggagggaga atctgtggca ggaaccgcct ctggtatagg gggaggcgaa
     8461 gatagcatta tttcctctcc ctcttcttcg ctgtaccaga taaccggcac cattaattta
     8521 tagccgcgct ttggtacagt agcgatatag acaggactat cttcatcatt atcttttaat
     8581 gacttacgta gttctgagat actctgcgtc acaacgtgat tggtgacaat acttctcttc
     8641 cagacattat cgataagttc atccctgcta agtacttcgc cactgtgttg agcaaagaaa
     8701 accagaagat cgattaatct cggctcaagg gtaagttgac gcccattgcg gctaatttgg
     8761 tttatggacg gagtaacaag ccattcgcca acgcgaacta caggttgttg cataatagaa
     8821 actcattcga aaagggaatg atgcaatgat aattgccaca acctattttt accatctata
     8881 gatgggttta tttacatatt attagtgaat gcaagacgtt atttttacca gccataaact
     8941 tcttgattac atagtattac gaaaggattt tactgagaac cagaagtaat attccttacc
     9001 atcaaaattc atcatctttg ccaaagaaaa atgttcagaa aataatccat ggaaaattgt
     9061 ccggagcact tactatttta atggattgtt agtctttgca tgagcaagcg aactgataca
     9121 tttctctttg ttctcattca gaaaatctca tcagtcgccg ttcagaccgg tgttgtgctt
     9181 tatggatgcg ttacgctcct gatgacgtca tttgacgttc aacagcatca cggggccgca
     9241 cgacatttca cgtcagttag tgctatagct caggaacaaa ttttcccgaa ttgggatatg
     9301 cccgcaaatt gctggtgatg tggggagaat ctggttgagt tcggtagaat tgatttggag
     9361 gcagaacgct taaatcgtgg cgtcctgaaa cgaaaaacgg acctccgtgg aggtccgttt
     9421 atatgaattt ggtgcccgga ctcggaatcg aaccaaggac acggggattt tcaatcccct
     9481 gctctaccga ctgagctatc cgggcaacgg ggcgcattaa accgtaatct gcacatctcg
     9541 tcaacctaat ttcagtaaaa gcgattcaac tgcttaagat tgcggcaaat cgcttctttt
     9601 ctgtgtttct gtcaggtcaa tgcaccaccc tggcggcagc gggcgaagcg caggatatct
     9661 tccgccagcc gatgtgcagt gtcgacatcc gcctggctac gattcaccag cattcggctt
     9721 aaacagcctt ccagcaccag ttccatctgc tttgctacca tcgccggatc gtcaacttcc
     9781 agtgtggtta acagttcgtg ggtgaaatcg taggccgcgc ttttttgctg atcggccagt
     9841 tgatgaatag ggtggccagg atcgggataa aacgtacagg cagcgataaa tagacagccc
     9901 ggatagcggt tgtttttaac gcactccgat aacgcctgat aacgtgccag cagcttttgt
     9961 tcggcggttt gcgtttcgtc cagcatcagc tgacgacgcc agacatctat ctgttggcta
    10021 agataacgca gcgcatcgta gaggattgcc tctttgtctg gccagaagcg gcgtagctcg
    10081 tccagtggat aatccacacg ttcagcaacc atctccagcg tggtgttggc aatcccttgt
    10141 aattctaata atttcagggc ttctcccagt acatcttcac gttgcacgct attttcctcc
    10201 gtctttccca ctgcaagtgt cgttcacggt tggcgatcgc gcaaatgtgc gctgaaggtt
    10261 tcagcatcca taaagcccgt gacgcgtgct tgtggatgct cctggccttg tccgtcaaaa
    10321 aagagaattg tcggtaggcc aaggacatta agatgcttta acagcgccac atcttgtgcg
    10381 tcgttggccg tgacgttggc ctgaagtaag accgtgtctg ctaacgcttt ttgcacctgc
    10441 gggtcgctga aggtgtattt ctcaaactct ttacaggcga cgcaccagtc ggcataaaga
    10501 tctaacatca ccggtttgcc tttggcttca acgagcgcct gatttaactc atctaccgtt
    10561 ttgatttgtg taaagttgag atgcgtctga gtttgcgcgg tatgcgtcgc accaaatgcc
    10621 caatcctgaa gtgggcgcac gctaaccaat gccgctgcca gcagaataat ttgcacaata
    10681 cgcatccagc cgcgtttagc ctgtaggctg gtgataaagg cccagccaaa gaatgcgaca
    10741 cccagcgccg accacaagcg taatccccat acatcaccaa tcactcgctc cagcaggaag
    10801 accggcagtg cgaggatcac aaaaccaaac gcggttttga cttgttccat ccacgggccg
    10861 cttttcggca gcaagcggtt accaaagacg gtaattagca tcagcggcag gcccatgccc
    10921 aacgcataga gataaagcgt gccgccgccc agccacatgt tcccgctttg ggcgatatac
    10981 agcagaatcg cgctaagcgg tgcggtggtg catggtgaac agatcagtcc ggcaatcgcc
    11041 cccataacaa acacaccgcc aggtgagccg ccctgttggc gattgctcat caacgtgaga
    11101 cgtgtttgca gcgaagaggg gagttgcagg gtaaacaagc caaacattga catcgccagc
    11161 aaggtaaaga cgatggcgag gccaatgagc acgtatgggt gctgtagcgc cgcctggaac
    11221 tgtaaccctg cggcggcaac caccagaccc agcgccgtgt aggtcagcgc catcccctgc
    11281 acataaataa aggtcagcaa caatgctctg gcagtggaga gccgctgttt accacccagc
    11341 acgatgccag aaatcagtgg gtacattggc agcacgcatg gcgtaaaggc gataccaata
    11401 ccgatcaaca acgcccagag cgcggaaaag ggcaattgcg cggtgggctg ctcttgctgc
    11461 ggaacagaca caggctgtgg cgctgcgttg ttggcgacca cttcgcttaa cggaacggtt
    11521 ttggtttctg gcggataaca gaaaccggca tcagcacagc cctggtaggt gacagttaac
    11581 gtcgctcccg cactcgcctg gttgatggtg acgggaagcg tcagccgatc gcggtaaatc
    11641 tcgcttttgc cgtaaaactc atcttcatgc cagacgcctt gcggcagctg cacgtcggca
    11701 attttcgcgt gttccggcgt aatgcggatc tgtttacggt agaggtagta accgtctttg
    11761 atctgccagg tcagattaag gtcatgttgg ttttgctgaa aatcaaaagc aaaggcttga
    11821 tccgcgggga caaattgtga acgtcccggc gcgtcgaata atccggcaaa aacggaagtg
    11881 ctgcaaagta gcaggatcag cgtaaagatg cgttgagcca tgagaggtaa tctgtgtctc
    11941 cgtgtgtaac aggtaaaacc agaagttccg gggtttgata tggatgatga gacttcaggc
    12001 attccagcag tgcctgctgg tgagatacgg tagtttttaa aatcatctgc acttcgtatt
    12061 cttgctccag cttaccttcc cagtaataga gagaggtagc gccggggatc aaggtcgcgc
    12121 aggccgccag tttttccgcc agcactttgg cggctaaatc ctgggctgtc gcttcatctg
    12181 gtgccgtaca tagcaccacg acagacgcgg tattcgaact tttttcatca agcataaaca
    12241 cctcgcaaga acagatggaa ccgcaagaga gaggtcacta tacaacggac gggggaagga
    12301 tgttagtcgg cgggaaagaa agcggggcgt gaacgccccg cgattgatca ttacagcatg
    12361 aagctaccca gcacgaagcc gaagcaaacg gccagggcaa cacccagagt acccgggatg
    12421 aagaacggat ggttgaagac gaatttaccg atacgggtag tacccgtgtc atccatctgt
    12481 accgcagcaa ccagcgtcgg gtaggtcggc agaatgaaca gaccagacac cgcagcgaaa
    12541 gaagcaacag cggtcagcgg tgaaacgttc agtgccagag ccatcggcat cagtgctttt
    12601 gcggttgcag cctgagagta cagcagagca gaagcaaaga agaagatgac ggccagcagc
    12661 cacggatgac cctgaatcac ttcaccagcg gtatctttga tccagtcgat gttgttggaa
    12721 acgaaagtat cgcccagcca cgcaacaccc aggatacaaa tacaggcgct catacctgct
    12781 ttgaaggtgc tggagttgag gatgttgtcg gtatcgactt tacagataac ggtggtcaga
    12841 gttgcaacgc tgagcatgat gatcaggatt gcgttggtgg tgttcatcag cggtttttca
    12901 accagaccca tgcttgggct gttgatgatt gcatagataa ccacgccaac tacgcccagc
    12961 aggaacagcc agacggacgt ttttgcaccg gatttgattt caatctgctt ttcaccgcgc
    13021 agttcaacca ggccctcttc cagacgcttg cgataaatcg gatcgtcaga gagtttggag
    13081 ttgaacagca tagtgaccag gaaggacatc accagaaccg ccagcagggt ggacgggatg
    13141 accacggaga gcagatggag gtagctgatg ccatgacctt ccatcacgga agacatgtaa
    13201 accactgccg ctgagattgg cgatgcggtg atcgcaatct gcgcggatac cactgcagta
    13261 gacagcggac ggcaaggttt aacgccttgt tccttcgcaa cttcagcgat aactggcagt
    13321 gtcgccagag agatgttgcc agtaccagca aagatagtca ggaaataggt cacgatcggt
    13381 gcgaggatcg tgatgtattt cgggttacgg cgcagcagct tttctgtctg atgaaccaga
    13441 tagtccagac cgccagcaac ctgcatggca gaaatagcgg cgataaccgc catgataatg
    13501 gagatgacat cgaacgggat gttaccgggt ttaacgccaa tagcggcaag aaccagcacc
    13561 cccaatccgc ctgcaaaacc aatacctatt ccccccaatc tggcgcccaa gaagatcgcc
    13621 agcaaaacta tgatgagttc tacaactagc atattagcct tccttgtttt ttaacaagtt
    13681 gatattagat tgttattttt aagttactgc tcacaagaaa aaaggcacgt catctgacgt
    13741 gcctttttta tttgtactac cctgtacgat tactgttcgc tttcatcagt atagcgtttt
    13801 gctttgtaag ccgggtgcat cagattctgt acggagaaaa tatcgtcaag ttccgcttca
    13861 gtcaacagac cgcgttccag aacgacttca cgtacactct taccggtttc ggcacagatt
    13921 ttacccacga tgtcaccgtt gtggtgaccg atgaacgggt tcaggtaagt aacgataccg
    13981 atagagttgt aaacgtaacc ttcgcacact tctttgttag cagtgatgcc gttaatgcat
    14041 ttttccagca ggttgtagca agcgttggtc agaatgtgaa cggattcgaa catggcctgg
    14101 ccaatgaccg gctccataac gttcaactgc agctgacctg cttctgctgc catggtaaca
    14161 gtggtgtcgt taccgatgac tttgaagcat acctggttaa ccacttccgg aacaaccggg
    14221 tttactttag ctggcatgat ggaagagccc gcctgcagtt ccggcaggtt gatctcgttc
    14281 aggccggcac gtgggcctga agagagcaag cgcaggtcgt tacagatttt ggacatcttc
    14341 acagccaggc gtttcagcgc gccgtgaacc ataacataag cgccgcagtc agaggtcgct
    14401 tcgatcaggt cttcagccgg tacgcatggg aagccagtaa cttcagccag ttttttcact
    14461 gccagcggag agtactcttt cggcgtgttc agaccagtac cgattgctgt tgcaccaagg
    14521 ttaacttcca gcagcagttc agcggtacgt tggatgtttt tcacttcttc tttcagcagg
    14581 atgctgaaag cgcggaattc ctgaccgagg gtcatcggta ctgcgtcctg cagctgggta
    14641 cgacccattt tcaggatgtc ctggaattcg acagctttac gttcaaagcc ttcacgcagt
    14701 tggttaatcg catctaccag cttaatcagg gaagagtaaa ctgcgatacg gaaaccggtc
    14761 gggtaggcgt cgttagtgga ctgacatttg ttaacatggt cgttcgggtt caggtactga
    14821 tattcacctt tttggtgacc catcagttcc agaccgatat tggccagcac ttcgttggtg
    14881 ttcatgttta cggaagtacc tgcgccgccc tggtagacgt ctaccgggaa ctgatccatg
    14941 cattttccgt tgttcaggac ttcatcacat gcggcaatga tggcattcgc tacactttta
    15001 ggaatggttt gcagctcttt gtttgccata gctgcggctt ttttaaccat taccataccg
    15061 cgaacaaatt caggaatatc actgattttg ttgttgctga tatagaagtt ttcaatcgct
    15121 ctcagagtgt gaacaccata gtaggcatca gctggaactt ccctggtacc caacagatct
    15181 tcttcgatac gaatgttgtt tgacatgtga accttctttt tcaagctgcc aatgatttgc
    15241 tttaaacaca cagaatatat gtggtttcga atgtttttcg accgacgatt atcccctgca
    15301 tcgaccgaat acccgagatc atatgctgct tgaggatttc taccgtaatc tggatcactt
    15361 taagtgtcgg tttttacccc ttaattatta atttgtgaaa tagatcaccg ctttgggatt
    15421 actaccaaaa atagttgcgc aaacatcttg aaattttgct aatgaccaca atataagctg
    15481 aacgcgattc gcaacccatt caggtagccg ggttttaccg gctgctatta caggagaaac
    15541 ctttgcgctg gttacctttt attgccattt tcctttatgt ctatattgag atttcaatct
    15601 ttattcaggt tgcccatgta ttgggggtat tgctgaccct cgtgctggtt atattcacgt
    15661 cagttatcgg gatgtcactg gtacgtaacc agggctttaa gaatttcgtg ctgatgcagc
    15721 aaaaaatggc ggcgggtgaa aacccagcgg cggagatgat taaaagtgtt tcgctgatca
    15781 ttgccggttt gctgctttta ttaccgggct ttttcaccga cttcctcggt cttctgcttt
    15841 tattaccgcc ggtgcaaaag catctgacag tgaagttgat gccgcatttg cgcttttctc
    15901 gtatgccagg cggcggtttt agcgccggga ccggtggcgg taatactttt gatggtgagt
    15961 accagcgaaa ggatgatgag cgcgaccgcc ttgatcataa agacgatcgc caggattaat
    16021 gtcgaaacgc cggattatgt ggttatgcca ttttccggcg tttttcgttt tggcagcaac
    16081 agccataacc ccgccagcat gatcagcgca tagagacttt tccagccgac cattgccagt
    16141 aacagaacgc ataacagccc gccaaccacc gccagtagtc gataacgtcc ttgcaataat
    16201 ttacagcctg ccagcatgca taacagataa atcataataa agatgccatt ggcataaata
    16261 ataagagcgt ccagattgat ctctaaagca tgaatcacca aagtgctcac cacgcagcag
    16321 ccgagcaccg cattgagggc attattcggg atatggcgag aagagaggcg tgccaggtag
    16381 tggtcaggat tatgttgcgc ctgcgaccag accaggcggg cgaagctctg tatataaatg
    16441 ttgagactgg caaagcaggc cagatagcca attacgcagg caatccataa cgctcctaca
    16501 ccgaacagtt gcactacaat ttttggaagc gatgctgccg cggccatttg ttcaccatag
    16561 gcgtcgaagt gtaagacgac taccgtacag ccccagtaga ctaatcctgc cagcagcaga
    16621 ccaatcatca aagcacgagg aaaatcacgt tctggatttt taaattccga ggcaagatgg
    16681 gcaaatgctt ccagaccgac aaaacaccag aacatcactg ataacgcagc aaataacccg
    16741 gtaagttcga tatttcctgg cgcggggaag gggatactcg caggtttgat atcgcccgcc
    16801 caccagatag cgacaatcag tgcgacgata agtccggcaa taacggtttg tagatttgca
    16861 ctggaactgg caccgcgagt accgatatac cacaccaacg ccagcgtacc gagttctgcc
    16921 aacaacagtt gccagctatg ccagccaaac atcgcctggc cgaacccggc ggcaatttgt
    16981 agcgcggcag gcaaacccac gggaatgacc gataaaaaca gccagccggt gactcgctca
    17041 agccgcgaac caaacgccat accgacgaag tgcgcgacgc cgcctgcact gggatagtgg
    17101 cgacccagaa tcgcaaacac aatcgcaatc gggaacacta agataatcaa aacgggccac
    17161 gcccacaggc tgttattgcc cgctaccagc gcagctaacg caggaacggc aaacacgcca
    17221 gtgcctaata atgacgtcga tagcagtcca atgccctggg ccagccccag ttcttgtttg
    17281 agtccactca tgggttgata tccgattgcg cccaaatttt gggcaactgc gtagattttc
    17341 gatggtagca caatcagatt cgcttatgac ggcgatgaag aaattgcgat gaaatgtgag
    17401 gtgaatcagg gttttcaccc gattttgtgc tgatcagaat tttttttctt tttccccctt
    17461 gaaggggcga atcctcatcc ccatttctct ggtcaccagc cgggaaacca cgtaagctcc
    17521 ggcgtcaccc ataacagata cggactttct caaaggagag ttatcaatga atattcgtcc
    17581 attgcatgat cgcgtgatcg tcaagcgtaa agaagttgaa actaaatctg ctggcggcat
    17641 cgttctgacc ggctctgcag cggctaaatc cacccgcggc gaagtgctgg ctgtcggcaa
    17701 tggccgtatc cttgaaaatg gcgaagtgaa gccgctggat gtgaaagttg gcgacatcgt
    17761 tattttcaac gatggctacg gtgtgaaatc tgagaagatc gacaatgaag aagtgttgat
    17821 catgtccgaa agcgacattc tggcaattgt tgaagcgtaa tcctcgcacg acactgaaca
    17881 tacgaattta aggaataaag ataatggcag ctaaagacgt aaaattcggt aacgacgctc
    17941 gtgtgaaaat gctgcgcggc gtaaacgtac tggcagatgc agtgaaagtt accctcggtc
    18001 cgaaaggccg taacgtagtt ctggataaat ctttcggtgc accgaccatc accaaagatg
    18061 gtgtttccgt tgctcgtgaa atcgaactgg aagacaagtt cgaaaacatg ggtgcgcaga
    18121 tggtgaaaga agttgcctct aaagcgaacg acgctgcagg cgacggtacc accactgcaa
    18181 ctgtactggc tcaggctatc atcactgagg gtctgaaagc tgttgctgcg ggcatgaacc
    18241 cgatggacct gaaacgtggt atcgacaaag ccgttaccgc tgcagttgaa gaactgaaag
    18301 cgctgtccgt accgtgctct gactctaaag cgattgctca ggttggtacc atctccgcta
    18361 actccgacga aaccgtaggt aaactgatcg ctgaagcgat ggacaaagtc ggtaaagaag
    18421 gcgttatcac cgttgaagac ggtaccggtc tgcaggacga actggacgtg gttgaaggta
    18481 tgcagttcga ccgtggctac ctgtctcctt acttcatcaa caagccggaa actggcgcag
    18541 tagaactgga aagcccgttc atcctgctgg ctgacaagaa aatctctaac atccgcgaaa
    18601 tgctgccggt tctggaagct gttgccaaag caggcaaacc gctgctgatc atcgctgaag
    18661 atgttgaagg cgaagcgctg gcaactctgg ttgttaacac catgcgtggc atcgtgaaag
    18721 ttgctgcggt taaagctccg ggcttcggcg atcgtcgtaa agctatgctg caggatatcg
    18781 caaccctgac tggcggtacc gtaatctctg aagagatcgg tatggagctg gaaaaagcaa
    18841 ccctggaaga cctgggtcag gctaaacgtg ttgtgatcaa caaagacacc accactatca
    18901 tcgatggcgt gggtgaagaa gctgcaatcc agggccgtgt tgctcagatc cgtcagcaga
    18961 ttgaagaagc aacttctgac tacgaccgtg aaaaactgca ggaacgcgta gcgaaactgg
    19021 caggcggcgt tgcagttatc aaagtaggtg ctgctaccga agttgaaatg aaagagaaaa
    19081 aagcacgcgt tgaagacgcc ctgcacgcga cccgtgctgc ggtagaagaa ggcgtggttg
    19141 ctggtggtgg tgttgcgctg atccgcgtag cgtctaaact ggctgacctg cgtggtcaga
    19201 acgaagacca gaacgtgggt atcaaagttg cactgcgtgc aatggaagct ccgctgcgtc
    19261 agatcgtcct gaactgcggc gaagaaccgt ctgttgttgc taacaccgtt aaaggcggcg
    19321 acggcaacta cggttacaac gcagcaaccg aagaatacgg caacatgatc gacatgggta
    19381 tcctggaccc aaccaaagta acccgttctg ctctgcagta cgcggcttct gtggctggcc
    19441 tgatgatcac caccgagtgc atggttaccg acctgccgaa aaatgatgca gctgacttag
    19501 gcgctgctgg cggtatgggc ggcatgggtg gcatgggcgg catgatgtaa ttgccctgca
    19561 cctcgcagaa ataaacaaac ccccgggcag aaatgtctgg gggtttttct tttggtcatc
    19621 tttctagtat aagattcaga cacggacgac gcgagtggcg tccagctcat tgattatggg
    19681 gaataacatg cacgtaaaat acttagcagg gattgtcggt gccgcgctac tgatggcggg
    19741 ttgtagctcc agcaacgaat tgagtgctgc cggtcagagt gtacgcattg tggacgagca
    19801 gccaggcgca gagtgccagc tgattggtac tgcgacaggt aagcaaagca actggctttc
    19861 cgggcaacac ggagaagagg gcggttctat gcgcggcgca gcaaacgatc tgcgcaacca
    19921 ggcggctgca atgggcggta acgtgattta tggcatcagc agcccgtcgc agggaatgtt
    19981 gtccagtttt gtcccgacgg atagccagat tatcggtcag gtttataagt gcccgaactg
    20041 atgtgatcca gaggctgggt gacaaacgta ttattgcctg atgcgcttcg cttatcaggc
    20101 ctacagcagg cattgcaact gattgaatgc ttgagaattt gtaggccgga taaggcgttt
    20161 acgccgcatc cggcatgacc aacgatcacg ttatcagtca tttcaggctg gatggctcca
    20221 gcctcgcatt agcaaagatg agattatttc gcctgtggtg cagttttgct ggtggattga
    20281 acacgtaagt gatgcagcgc gcgtaagcat tgatcctggg ttagcggttg ggcgttattc
    20341 gccgctacgg tgcgaacata gacctgacat ggcacacccg cgagtttttt cagtcgtggg
    20401 ctgaaatcaa gcattcggcg agtgatcgtc tctatttctt tacccggcgt tgcttcacgc
    20461 gttttcacca ctgcgccgct aaattgttct ttgccacttt catctttaaa gcggtaaagc
    20521 accgccagat aatggtcgaa caggttatgt ttccattccg gttgggaata ggtgtcatat
    20581 tccagattgc cattttcttg gcagtcaacg tcatataacg gcaggaaatc taacagtgag
    20641 gtaatacgta atgccagttc acgcatttcc gcattattaa ttgcatgaat gattgctcgc
    20701 gccagcattt caatctgcaa ctcattgacg actaactcac atttgctacc gtcatgtagc
    20761 gttaatacga aggtaagatt ttcgccttga ttatcggtaa gttccagcgt gttaacccga
    20821 cgattaatat cggcattttt aagctcatca accagaattt ctgggatgtt ttctgccatt
    20881 ttcttaatga ctttatcgcg agcttgctca tactgaaggc gtgcagcagc atccagttta
    20941 tgcttttgat gcagacgact ttccagcgca atcagcaggt cgcgcagttc catgacagac
    21001 ataaagaaca acgattcttt attgcgaggt tctttaattt tcagcgccag tgcaatgaaa
    21061 ttattactct tgcgaataac accggtatta acacccttga tgcttatggc catgtaggtt
    21121 ctccctaacc atttctcaat taaataatta attttaattt ataagccaga taaatgggct
    21181 tggtagtaat agttgttaaa ataacataaa tagccgtgct cactctatat aaacagaaaa
    21241 gaaaggttaa ttattggtgt tagctatata aaaagtaaca acagcaatgc atatgaataa
    21301 ttaaaggtta tgcaacgtgc aaagattaaa tttctcagtt taatgacagg ttgctgtact
    21361 tgcgtttgtt gctcttgccg gatgcggcgt aaacgcctta tccggcctac agttcattgc
    21421 aaatccattc aatttcaatg cacgatgtag gcctgataag cgtagcgcat caggcaattt
    21481 taatgtttaa cttccctgtt taatcagtta ctgctggcgt agctggagat ccagcggcgt
    21541 tttgctgggt tcgccgccaa tttctcgcgc cagtttcggc accagatatc ccgacaccaa
    21601 tgtcagcaac tcacgcataa tctgccgtgc ttcgtcatca ctcaccataa aatgcgccgc
    21661 gccctgtact ttatcgagca catgcaggta atacggcatc acgccggcat cgaacaacgc
    21721 attactcagg tttgccagcg tttgtgcgtt atcgttcaca tcacgtaaca gaacgctctg
    21781 gttcagcaaa gtaacgccta cccggcgcaa cttagccatc gcctgacgga atgtttcatc
    21841 tatctcattg gcatggttga tgtgattcac cagcaagatt tgcagcgtag aatgtgaaaa
    21901 gcgttcaacc agcgcatctg tgatgcgtgc cgggatcaca atcggcagac ggctgtgaat
    21961 ccgcagacgt ttgatatgcg ggatggcttc cagttgtgtg agcaaccagt ccagctcgtg
    22021 atctttcgcc atcagcggat cgccgccaga gaaaatcatc tcgtccagtt ccggatgcgc
    22081 agcaacatac tcaagtgcag tctgccagtt acgcttgttg ccctgatttt cggcataggg
    22141 gaagtggcga cggaagcaat agcggcaatt taccgcgcag ccgcctttga ccagcaaaag
    22201 cgcccggttg tggtatttat gcaacaaacc aggcactacg ctgtgctgtt cttccagtgg
    22261 gtcggtggag aatccggacg cgacgacaaa ctcatcttgc gaggtaagta cctgacgcaa
    22321 aagaggatcg tccggattgc ctttctccat gcgatcgata aatgagcggg gcacacgcag
    22381 ggcaaacagc tttttggcgc tgcgtccggc caacagtttt tcgtccgcgt ctatattcaa
    22441 aagacgcaga agttcatcag gatcggtcac aacatcggca agttgcgtta accaatcttc
    22501 tctggatggg gtatttaggg ttacaatatg cgccattttg tggcttagct accaattaac
    22561 aaatttcaga gggccttatg gcaacgtact atagcaacga ttttcgtgct ggtcttaaaa
    22621 tcatgttaga cggcgaacct tacgcggttg aagcgagtga attcgtaaaa ccgggtaaag
    22681 gccaggcatt tgctcgcgtt aaactgcgtc gtctgctgac cggtactcgc gtagaaaaaa
    22741 ccttcaaatc tactgattcc gctgaaggcg ctgatgttgt cgatatgaac ctgacttacc
    22801 tgtacaacga cggtgagttc tggcacttca tgaacaacga aactttcgag cagctgtctg
    22861 ctgatgcaaa agcaattggc gataacgcta aatggctgct ggatcaggca gagtgtatcg
    22921 taactctgtg gaatggtcag ccgatctccg ttactccgcc gaacttcgtt gaactggaaa
    22981 tcgttgatac cgatccgggc ctgaaaggtg ataccgcagg tactggcggc aaaccggcta
    23041 ccctgtctac tggcgctgtg gttaaggttc cgctgttcgt acaaatcggc gaagtcatca
    23101 aagtggatac ccgctctggt gaatacgtct ctcgcgtgaa gtaatgcggt tgtggtgcgg
    23161 cctgcaggct gcaccatcac ttattcaggt cagagatgat gaaacgcctt atcgttcttg
    23221 ttttgcttgc cagcacgctg ctcacgggct gtaacaccgc tcgcggtttc ggcgaagaca
    23281 tcaaacatct cggcaactcc atctctcgcg ctgccagcta atttttcttc tcttccgaaa
    23341 aatcatcaga tttccgtcat ttttggcgat gttgtctatt attaatttgc tataggcaaa
    23401 cataaataac attacctaaa aggaagacgt tatggtgaag aagacaattg cagcgatctt
    23461 ttctgttctg gtgctttcaa cagtattaac tgcctgcaac accacgcgtg gcgttggtga
    23521 agacatttct gatggcggta acgcgatttc tggcgcagca acgaaagcgc agcaatagca
    23581 ataagcaata acggtacgac agctgtgtcg tgccgtttgt tttttctgcg atagtcacta
    23641 aggtaatagt tgaaattccc ctgccacctg gcaaaatatc cgttcaacca tcagctttgc
    23701 aggacgacct gcaaacgcgt cttttcaccg gggacggccc caattctccg gagcctgata
    23761 tgtcctggat tatcttagtt attgctggtc tgctggaagt ggtatgggcc gttggcctga
    23821 aatataccca cggctttagt cgtttgacgc cgagtgttat taccgtgacg gcgatgattg
    23881 tcagtatggc gctacttgcc tgggcgatga aatcgttacc agtagggacg gcttatgccg
    23941 tgtggacggg tattggcgca gtcggcgcgg ctatcaccgg cattgtactg ctcggtgagt
    24001 ccgctaaccc gatgcgcctg gcgagtctgg cgttaatcgt attggggatt attggtctga
    24061 aactcagcac tcactaacta ccgggctgct gtacccaaat aaacttactg acatcaaacc
    24121 cttcccgggt cgcgactgcc agcatctcct gtttcacttc gtcagaaatg gttggcgtgc
    24181 gggagagtat ccacaggtag tcgcggtccg ggccgcaaac cagcgcatgg cggtattccc
    24241 gatcaagtgc aatgacgtta taaccgccat agaaaggacc aaagaatgac actttcagcg
    24301 cagcgcgtgt tggtgcgccg gtaaagtacg ctttcccttc actctgctgc cacatttctc
    24361 tgtcagggtt atagccttta ttgatgacat tcaggccgcc gtcatcacgc agactgtatg
    24421 ttgcggtgac tttttccagt ccacgttcaa aacggtgatc aaaacgggca atctcatacc
    24481 aggtaccgag atagcgttta gcgtcgaaat tattcactac tgtcacgcca cgcggcggcg
    24541 taggagaact gcaggcaacg accagaaatg cagctgtcgc tgcggcaacg agagggagca
    24601 ggcgcataaa tgtttcctta ctggtttttt tctaagtgta gatgacagca aggaaaagcg
    24661 cagaaaaggt ccgaaaattc ggacccgatg aaattttact gtagagcgtt gagaatctgc
    24721 caggcggcgg tgactctcgc tggattggga tagtttttgt tagccagcat cactataccc
    24781 agctcttttt ctggaataaa cgcgacatag ctaccaaatc cgccggttgc ccctgtttta
    24841 tgtacccatg atgcgcgtac tgcaggagtt gggggcgtaa tcgcttttac ggggcgtgct
    24901 gccagtgcaa ttttattgcc actgccgtta atgatgctgt caggatttac cggccagtcc
    24961 agcatttccc agcccaggcc ctgatacata tcgccggttt gccagtagcg agattgtgcc
    25021 agttgtatcc cttgctgaag tgttttgtcg ttgatatcac ggggattcat attgcttcgt
    25081 acccagcagg ccatatcttc aatggtcgac ttcacaccat aagtttcagc atctaacgcc
    25141 cctggcgaaa catgaactgc cttgccttcg cgatatcccc aggcgtaatt cttttcttct
    25201 gcgggcggta cattaatcca cgtatggttg agtttgagtg gctggaagac acgtgtttgc
    25261 atcgcctgct caaaactcaa accagacggc ttcacagcca gtgcgccgaa caaaccgata
    25321 ctggagttgg catacagacg ttgtgttcct ggagcccatg caggctgcca gttttgatag
    25381 aagcgcagca agtcgcttga ggatttcacc tcatccggca cctgcaatgg caggccgcca
    25441 gcggtgtagg ttgcgagatg taatagtgtg atcccgttcc actgtttagc ggtaagttca
    25501 ggccagtatt ttgttgcggg atcgcttaac ttgatttccc ctcgagcaat agcgtcgcca
    25561 ccaagcacgc ccgtaaatgt tttgctgacc gaacctaact caaacaacgt ttgctgtgtg
    25621 acgggctgct ttttggcgat gtccgcatag ccccaggtaa agtaataagg tttaccctga
    25681 taaattaccg ccaccgccat accggggatc ttttgttgct ctataagcgg ggtaattgtg
    25741 cgatgcacaa tatcgttgat tttttggggg gcagcaaatg tggagcaaga ggcggtaatt
    25801 aataaggcgc agagcgtcgt tttgaacata gggtctggtt tccatacaaa acggcccgcc
    25861 ataggcgggc cggatttaca ttggcgatgc gttagattgt aacgacacca atcagcgtga
    25921 caactgtcag gatagcagcc agaccgtaga aaacccattt gcccgcaggt acgtggattt
    25981 tcagatcgtg catcgcgtgg tgcatacggt gtaaaccaca ccacagcggc agaacgatca
    26041 tcaggaacag gaatacgcga ccaatgaagc tctgcgcgaa cgccagaacg cgctcgtagc
    26101 tcagtgcatc acccggaaac agccacagcg gcagcagaat acccaccagc aggatcatca
    26161 ccggcgcaat aattgcgctc cacataccac cggccccgaa gaggccccag aataccggtt
    26221 cgtcagaacg ctttggattt ggattgatca tctcaggctc cttaccagta cagggcaaca
    26281 aacaggatta cgatggtggc aaccacagtt accgcccaga gacttttgat aattggctct
    26341 ggacccattt tttcgtcttt tacaatgata ttggccgctt tcggtgccag ttcaaaccag
    26401 gttttggtgt gcagcagagc tgccgccaga gtgatcaggt taatgatcac aataaccggg
    26461 ttttgtaaaa agtcgacgaa tcccgcccag gcttccgggc catttttcag ggcaaacagc
    26521 ccgaaaatca gttcaatgct gaaccacaca gccggaaccg ccgtgccttc gcgcagcatg
    26581 taaaagcgat aaaacggcaa ttttttccac caggtggacg tcattggccg tacatacggt
    26641 ttacgtttag tcgtcatgtt gcactcctta gcgtggtttc agggtcgcga taagaaagtc
    26701 tttcgaactt tctactttgc cctgctgaat ggccgcagcc ggatcgacgt gtttcgggca
    26761 gacttcggag caatagccca cgaaagtaca gctccatacg ccgttctggc tgttcaactg
    26821 cgccatacgc tccttcttac cgtggtcgcg gctatcttcg ttataacgat gcgccagcgt
    26881 aatggcagcc ggaccgatga actctgggtt caggccaaac tgcgggcacg cggcgtagca
    26941 cagaccacag ttgatgcaac cggagaactg gtgatacttc gccatctgcg ccggggtctg
    27001 gatgttagta ccctgatccg cggtgcggga gttgccgatg atgtacggtt tgatcgcttc
    27061 cagactttcg atgaagtggg tcatatcgac caccagatcg cgttcaatcg ggaagttagc
    27121 taacgcttca accttcatac cgtcggtgta atcacgcagg aaggttttac atgccagttt
    27181 tggcacgttg ttaaccatca tgccgcagga accacaaatc gccatacggc aggaccagcg
    27241 gtagctcagg tccggtgcca ggttgtcttt gatgtagccc agcgcatcca gtaatgaggt
    27301 agttgcgtca taaggcactt catagaatgc gctatgcggt gcggtatcga cttccgggtt
    27361 atagcgcacc acctcaattt tcaggttttt catctcagcc attcgccttc tccttcttat
    27421 tggctgcttc cgccttatcg gctgcatccg cttcgccacc gtaaacgcgt ttagctggcg
    27481 gcagcgtagt aatcttcacg tcgctgtact ccaggcgagt cgtgccatca gcatcgcgga
    27541 aggcgagggt gtgtttgagg aagttgacgt cgtcacgctc ggtgcaacct tcgtccagac
    27601 gctggtgtgc gccgcgggac tctttacgtg ccattgcgga gtgcgccata cattcagcaa
    27661 cgttcagacc gtggcccagt tcaatggtgt agagcaggtc ggtgttgaac acgctggaag
    27721 tgtcggtgat gcgcacgcgc ttgaagcgtt cctgcagctc tgccagcttg tcgatggttt
    27781 tctgcatcag ttccggcgta cggtagatac cgcaaccttc ttccattgcc aggcccattt
    27841 cgtcgcggat cttcgcccag ttttcgccgc catcctggtt aaccagatct ttcagacgtt
    27901 gttcaacgcc agctgcctgc gcttcaattg ccgcttcgtt gccattaccg gcagttgctg
    27961 cacgctctgt cgcttgttca ccggccagac ggccgaagac caccagttcc gccagggagt
    28021 tggagcccag acggtttgca ccgtgcagac caacagagga acattcaccc acggcgaaca
    28081 gacctttaat gcgggtttca cagttctgat cggtttcgat accgcccatg gtgtagtgtg
    28141 cggtcggacg taccggaatc ggttctttaa ccggatcgac gccaacgtac gctttcgcca
    28201 gttcgcagat gaacggcaga cgttcatgca gttttttctc gccgaggtga cgcaggtcga
    28261 gataaaccac atcaccacgc ggcgtggaga tggtgttgcc tttacgccat tcgtgccaga
    28321 aggcctgaga aactttgtcg cgtggaccca gttccatata tttgtttttc ggctcgccca
    28381 gcggagtttc cgggcccatg ccgtaatctt gcagataacg gtagccattt ttgttgacca
    28441 gaataccgcc ttcaccacgg cagccttcgg tcatcaggat accggaacct ggcagaccgg
    28501 ttgggtgata ctgaacgaat tccatgtcac gcagcggaac gccgtggctt agcgccatac
    28561 ccataccgtc accggtaacg atgccgccgt tggtgttgta acgataaacg cgacccgcac
    28621 cgccggtagc cataacgacc gcgttagcac ggatctgcac cagcgtgcct tccatcatgt
    28681 tcattgctac caggccgcga acatgaccat catcaaccag aatatccagc acgaaatgtt
    28741 cgtcaaaacg ctggatctgc gggaattgca gagaggtctg gaacagcgtg tgcagcatat
    28801 ggaagccggt cttatcggcg gcgaaccagg tacgctcgat tttcatgccg ccgaagcgac
    28861 gtacgttgac gctaccatcc gggcgacggc tccatgggca cccccacagt tccagttggg
    28921 tcatttcggt tgggcagtgg tggacgaaat aatccacgac atcctgctca cacaaccagt
    28981 cgccacccgc tactgtatcg tgaaagtgat attcgaagct gtcatgatcc tgcgcgacag
    29041 cggcggagcc cccttctgca gcaacggtat ggctacgcat cgggtatact tttgagatta
    29101 gtgcgatttt tgcatttgga tttgcctgcg cggcagcaat tgcagcacgt aatcccgcgc
    29161 caccggcgcc tacaatggca agatcggctt gaaaggtttg cacgacattc ctccagattg
    29221 tttttatccc acagccccgt acttcagggt aagtgcctga aagttacggt ctgcgaacgc
    29281 tattccactg ctcctttata ggtacaacag tatagtctga tggataagtc tgaaatttga
    29341 cgagatcgat ttttttagtg cgcaagaggg ctaaattatc actgaagatg attaatttaa
    29401 ttactaaacc atcagatcgt gcttttttta gtcactgccc accgttgttt gattcctgcg
    29461 taaaatttgt ctcgccgtcg cgttgcgagt agacttcgtg cccttgtcaa aaactggaga
    29521 tttaactatg agcgaaacgg catcctggca gccgagcgca tccattccta acttattaaa
    29581 acgcgcggcg attatggcgg agatccgtcg tttctttgcc gatcgtggag tgctggaggt
    29641 ggagacgcct tgtatgagcc aggcgacggt aaccgatatt catttggtcc cgtttgagac
    29701 acgtttcgtt ggccccgggc attcgcaggg gatgaatctc tggttaatga ccagcccgga
    29761 ataccatatg aaacgcctgc tggttgccgg ttgtgggccg gtattccagc tgtgccgcag
    29821 tttccgtaat gaagagatgg ggcgttatca caaccctgag ttcactatgc tggagtggta
    29881 tcgaccgcac tatgatatgt accggttgat gaacgaggtg gacgatctct tacaacaggt
    29941 gctggactgc cctgcagcag aaagcctttc ttatcaacaa gctttcttgc gttatctgga
    30001 aattgaccca ctctctgccg acaaaacgca actgcgggaa gtggcagcga aactggattt
    30061 gagcaatgtt gctgataccg aagaagaccg cgacacgttg ctacaattgc tgtttacctt
    30121 tggcgtagag ccaaatattg gtaaagaaaa accgaccttt gtgtaccact ttccagccag
    30181 ccaggcatca ctggcgcaaa tcagtaccga agatcatcgg gtcgctgaac gctttgaggt
    30241 ttattataaa ggtattgagc tggcgaatgg tttccatgaa ttgacggatg cccgtgagca
    30301 gcaacaacgc tttgaacaag ataaccgtaa gcgcgcggcg cgcggtttgc cgcagcaccc
    30361 cattgaccag aatctgattg acgccttgaa agtcggtatg cctgactgtt ccggcgtggc
    30421 attaggtgtt gatcgtctgg tgatgttggc gctgggcgcg gagacactgg ctgaagtcat
    30481 cgcctttagc gttgaccggg cataattctg aaaaattact gaattaacag cgaagaatgg
    30541 cgtaataagc gccattctgt agcatatatt ttcatttttc cgctaaaggc cacaatttaa
    30601 gactggatat ttgatatcat ccaggtatca attctgtatt ggtttttacg ctgcctgctc
    30661 aatgttgcgc agtgcagttc agtgggcacg tgttatacac gcgctgaaat gaaggatggt
    30721 ttcatgcctc acacgataaa aaaaatgagt ctgataggac tcatattgat gatctttact
    30781 tccgtatttg gttttgccaa tagcccatcg gcttattact taatgggtta tagtgcgatt
    30841 cccttttata tattttctgc attgttattc tttattccat tcgccttaat gatggctgaa
    30901 atgggagctg cttatcgcaa agaagaaggt ggtatctatt cctggatgaa taatagtgtc
    30961 ggaccacgtt ttgccttcat tggtacgttt atgtggtttt cctcttatat catctggatg
    31021 gtgagtacct ccgcgaaagt ttgggtaccg ttctcaacat tcctctatgg tagcgacatg
    31081 acccagcact ggcgtattgc cggactggag cctacgcagg tggttggtct gctggcagtg
    31141 gcatggatga ttctggtcac cgtcgttgct tctaagggga ttaataaaat tgcccgcatt
    31201 actgcggtgg gcggtattgc agtaatgtgt ctgaatttag tattgctgtt agtaagcatt
    31261 actattttgt tattaaatgg tgggcatttc gcgcaggata ttaatttcct tgcatcaccg
    31321 aacccgggtt atcagtccgg tctggcaatg ctatcgtttg tggtatttgc catttttgcc
    31381 tatggcggaa ttgaagcggt tggtggtctg gtcgataaaa cggaaaatcc agaaaagaac
    31441 tttgccaaag gtattgtttt tgccgctatt gttatttcaa tcggttattc gctggcaata
    31501 tttttatggg gcgtcagcac aaactggcag caggtattaa gtaatggttc cgttaacctc
    31561 ggcaatatta cctatgtgct gatgaagagc cttgggatga cgctgggtaa tgcactgcat
    31621 ttgtcacctg aagcgtcatt gtcgctgggc gtatggtttg cgcgtattac tggactttcg
    31681 atgttcctcg cctataccgg tgcgttcttt acgctttgct attcaccgtt gaaagccatc
    31741 atccagggga cgccgaaagc attgtggccg gaaccgatga cgcgtctgaa tgcgatgggg
    31801 atgccgtcta tcgccatgtg gatgcagtgc gggttggtta ctatcttcat tctgctggtt
    31861 tcgtttggtg gcggtaccgc atcggcgttc tttaacaagc tgacgctgat ggcgaacgtg
    31921 tctatgacgc ttccttacct gttcctcgcg ctggctttcc cattctttaa agcacgtcag
    31981 gatctcgaca gaccatttgt gattttcaaa acgcgtatgt cggcaatgat cgcgacggtg
    32041 gttgtcgtac tggtggtgac atttgcgaac gtcttcacca tcattcaacc tgtggttgaa
    32101 gctggagact gggacagcac attgtggatg attggcggcc ctgtcttctt ctcgctgtta
    32161 gcgatggcga tttaccagaa ctattgcagc agagtggcaa aaaatccgca gtgggcggtg
    32221 gaataacccg atgccctatc gtctacctga cgatagggcg taaagacagg aaatgttatg
    32281 gatgacacca gccgggaccc ggcaataacc gaagatgaaa tcagggcgct gcaattttct
    32341 gccggggatg tagcggaaat agagcagacg attctctctt ttgtcgatgc ctgccacacc
    32401 cgtaaagtgg cgatggtggt gggtagcacc attaacaccc tcaaagatcg cgatggtaaa
    32461 cggtggggaa acctgccgga tatttattgc gcttatctta tccgatgtct ggtttttcgc
    32521 ggggaattag tgggatacgg cgatttgttt cgtatgcgct atagcgaaat taagcgaccg
    32581 gttaccttat gagtgcgcgc atgtttgtct tatgctgcat ctggtttatt gtggcgttcc
    32641 tctggataac catcacttcc gcgctggaca aagagtggat gattgatgga cgcgggatta
    32701 acaacgtctg cgatgtgttg atgtatctcg aagaagacga tacccgtgat gttggtgtga
    32761 taatgacgtt accgctcttt ttcccttttc tctggttcgc tttgtggcga aaaaaacgcg
    32821 gctggtttat gtacgccacc gcgctggcta ttttcggcta ctggctgtgg cagttttttc
    32881 tgcgctatca gttttgtttg tgagccggat tggttcatcc ggcacacaaa cttacaaact
    32941 tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag
    33001 gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg catggaagcc
    33061 agccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc
    33121 gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg
    33181 tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac
    33241 ttcttcgcta ttggcatcgg caggggccgg tatcgtcaac accacgcgcg tgaccgagtc
    33301 agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc
    33361 tttacggtcc cagtcgctga tggtggtggc gcgggtgtta attttcgtca cgctaccggt
    33421 gagatcgcga attgtcaccg tatcgccaat gcgaatcggt ttttcgaaca ggataatcag
    33481 accagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc
    33541 ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc
    33601 gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtaacccg tacccggcgt
    33661 taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggtaggttgc gcaccagctg
    33721 cgtggtgatg ataaatacca gaatggcaat tagcactgca ccgagggtaa tcggctccag
    33781 gctttctacg ccctgtacag tggaagtgac atcccacaga gaaatatttt cgaggaagcc
    33841 gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa
    33901 aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc
    33961 atcgacttca attgcccctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc
    34021 gcgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg
    34081 ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc
    34141 aaccgaggtt tcaagcctcg ctaacagcgc ctgtgccgtt gccagataac ccacagccga
    34201 cgccagaatg gcaaccaacg gcgcgccaat catcatgttc cacagcatat ggttggtaat
    34261 gttgtcgccg ctgccctctt tgttgagata cagtgggatc ccggcctttt tcaggctgag
    34321 ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga
    34381 gaattcacgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg gcacaataag
    34441 cccgatgctc atcaggtagt agcgcatccc acgggaaaca cgttcgcgcg gccagccaaa
    34501 atgagcgata aacaagccgt tcgggcgggc aaaggtggcg caaatcatca ctacccacag
    34561 cagcggcacg gtggccgtta caccatcgcc aatcgcgacc gccagcggat aaggccacgc
    34621 ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggt aacggtgacg cgacgagaat
    34681 cgaccagaaa agagtgcgca acgtcagcca gaagtgatcc tgagtcactt tgccaacttt
    34741 cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac
    34801 cagaatcaac gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca tcatcacact
    34861 ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat cctgggcgat
    34921 ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca gatagcggtg
    34981 cgttgcttcg ttcacctctt tcagcgcatc ctccagttgc ccgttggaga ctttcagctt
    35041 ggtcagttcc aatagtagcg tgtcgccacc ctgcaacaat gagttcagca actcacgctg
    35101 agtgcgcagt tgtgcttcca gaatacggtt ttgctcggca gtcagcggct gaccgtcggc
    35161 ctgatgaatt tgccgtagca gcggctgttt attaagcaga tcctcataac gtaaccgttg
    35221 cacacgcaac tgcgccattt cggtatcaag ctgttgtggt ttcggcattt ccggcagccg
    35281 tgccacctgc gcccgcagcg cttcgccgag cagattggac gatcccagcc attgcgactg
    35341 ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag cctgacgctg
    35401 ttgcgaggca acgagatcca tccgctgcgc ctgttgattc aaagccgccg atagttcgcg
    35461 gttaattttg aattgcgcga cgatatcttt cggcaaatcg gcgctgtttt ctgccagcaa
    35521 ttcggtactt tccagcgccc gctccgcctc aagctgacgt tggctgttta attgattacg
    35581 caaggcctgc aaatacgcat ccagttgctg gctctctttt tccgccagct ctgagcgtaa
    35641 gcgcgctaat tcctggcggt tattggcaga cagctgcgcc agctccagtt catcaacgag
    35701 cgccttaaga cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt
    35761 attgccggta agcgttccca ggcggcgctc gatctcattt aactgacggc gggcgtcggt
    35821 ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg
    35881 ctgggcctga cggcttttat ccagcaactg gctgctgacc tggagaattt cctgattcag
    35941 cgcgtcggta gacattcccg gcgacacgct gcgcggctcg tcacgcatgt tgtttaattg
    36001 tgcgcgcaga gtagcggaga gtttcggata attatcgata acttgctgat attgtttgat
    36061 gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgga gcgcctctac
    36121 gacttccggc tgtgcgggtt tcgccgcttt tgcctgctcc agttcctgag tgatttgttt
    36181 gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca tcagaaaagt
    36241 gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt ttttgtcgtc
    36301 aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg gggcaggttc
    36361 agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggccaa ttttcgtgac
    36421 agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca ggttgataac
    36481 ggtggaaccg agtttaaagc gacccatttc ctggcctttc agcagtgcca cagaaccgtc
    36541 gttttccccg gcaggccagg tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt
    36601 gcccgcccag accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat
    36661 tgggccaaat tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg
    36721 cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggcacgt agatcatctc
    36781 acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgtgggg agaggtaagt
    36841 ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg ccagcagggc
    36901 ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga ttttacccaa
    36961 ctggctgata acgccatcgg caggcatgac cagtacattc ggatcggtat cgattgggcg
    37021 tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc
    37081 cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat cgataaccag
    37141 ttttgtcagc catcctgccc gcttgcttgc gccccaaccc gccaggcgag taagccatag
    37201 tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag cctccaggcc
    37261 attgttttgt cgttcctgat ccggcctaca tgccggatcc tgaaaaaaag gggacgattc
    37321 taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac gcgtttttac
    37381 ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg cgattttccc
    37441 ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata
    37501 tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt gttccggctc
    37561 caggtgccag aggccgaact cacgcactcc tggggagtca atcacatcac cgccgtgcgg
    37621 gaagtgatac agccgagcgg cggtcgtggt gtgctgaccg agccccgagt tgtcagagac
    37681 atcgttggtc aggatctctt tttgcagccc cagcaacgca ttcagcaggc tggatttgcc
    37741 gacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt ccagcggttt
    37801 tagcccatcc tgagtatggc tggaaaccat caatacgcga taaccgatat tgcggtagat
    37861 atccatctgc tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag
    37921 cacaataatc gg
//
LOCUS       DACAPB010000022        37589 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.022, whole genome shotgun sequence.
ACCESSION   DACAPB010000022 DACAPB010000000
VERSION     DACAPB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.022"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..597
                     /gene="nrdB"
                     /locus_tag="IT904_001004"
     CDS             <1..597
                     /gene="nrdB"
                     /locus_tag="IT904_001004"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAP3190827.1"
                     /translation="NGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFAFAER
                     ELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDLFVQA
                     AQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIPWINT
                     WLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            597..851
                     /gene="yfaE"
                     /locus_tag="IT904_001005"
     CDS             597..851
                     /gene="yfaE"
                     /locus_tag="IT904_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAP3190828.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(905..1555)
                     /gene="inaA"
                     /locus_tag="IT904_001006"
     CDS             complement(905..1555)
                     /gene="inaA"
                     /locus_tag="IT904_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAP3190829.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            1734..1976
                     /locus_tag="IT904_001007"
                     /pseudo
     CDS             1734..1976
                     /locus_tag="IT904_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2018..3094)
                     /gene="glpQ"
                     /locus_tag="IT904_001008"
     CDS             complement(2018..3094)
                     /gene="glpQ"
                     /locus_tag="IT904_001008"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP3190830.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDNLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(3099..4457)
                     /gene="glpT"
                     /locus_tag="IT904_001009"
     CDS             complement(3099..4457)
                     /gene="glpT"
                     /locus_tag="IT904_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAP3190831.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            4730..6358
                     /gene="glpA"
                     /locus_tag="IT904_001010"
     CDS             4730..6358
                     /gene="glpA"
                     /locus_tag="IT904_001010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAP3190832.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            6348..7607
                     /gene="glpB"
                     /locus_tag="IT904_001011"
     CDS             6348..7607
                     /gene="glpB"
                     /locus_tag="IT904_001011"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAP3190833.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVHQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            7604..8794
                     /gene="glpC"
                     /locus_tag="IT904_001012"
     CDS             7604..8794
                     /gene="glpC"
                     /locus_tag="IT904_001012"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAP3190834.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            8987..9889
                     /locus_tag="IT904_001013"
     CDS             8987..9889
                     /locus_tag="IT904_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAP3190835.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLSNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFDRDQVLAATQLSEADLAANNH"
     gene            complement(9930..10733)
                     /gene="yfaU"
                     /locus_tag="IT904_001014"
     CDS             complement(9930..10733)
                     /gene="yfaU"
                     /locus_tag="IT904_001014"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAP3190836.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     EQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(10751..12041)
                     /locus_tag="IT904_001015"
                     /pseudo
     CDS             complement(10751..12041)
                     /locus_tag="IT904_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(12098..13303)
                     /gene="rhmD"
                     /locus_tag="IT904_001016"
     CDS             complement(12098..13303)
                     /gene="rhmD"
                     /locus_tag="IT904_001016"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAP3190837.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(13318..14100)
                     /locus_tag="IT904_001017"
     CDS             complement(13318..14100)
                     /locus_tag="IT904_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP3190838.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(14320..15522)
                     /locus_tag="IT904_001018"
     CDS             complement(14320..15522)
                     /locus_tag="IT904_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAP3190839.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15622..16164)
                     /locus_tag="IT904_001019"
                     /pseudo
     CDS             complement(15622..16164)
                     /locus_tag="IT904_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16443..16868
                     /gene="nudI"
                     /locus_tag="IT904_001020"
     CDS             16443..16868
                     /gene="nudI"
                     /locus_tag="IT904_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAP3190840.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16907..17509)
                     /locus_tag="IT904_001021"
     CDS             complement(16907..17509)
                     /locus_tag="IT904_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP3190841.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDITGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNDIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLNGLVMHVEKGKVYLDGEFVNH"
     gene            17817..18956
                     /gene="arnB"
                     /locus_tag="IT904_001022"
     CDS             17817..18956
                     /gene="arnB"
                     /locus_tag="IT904_001022"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAP3190842.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADHVITALQQLAGQ"
     gene            18960..19928
                     /gene="arnC"
                     /locus_tag="IT904_001023"
     CDS             18960..19928
                     /gene="arnC"
                     /locus_tag="IT904_001023"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAP3190843.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19928..21910
                     /gene="arnA"
                     /locus_tag="IT904_001024"
     CDS             19928..21910
                     /gene="arnA"
                     /locus_tag="IT904_001024"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAP3190844.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRILILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21907..22797
                     /locus_tag="IT904_001025"
     CDS             21907..22797
                     /locus_tag="IT904_001025"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAP3190845.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22797..24449
                     /gene="arnT"
                     /locus_tag="IT904_001026"
     CDS             22797..24449
                     /gene="arnT"
                     /locus_tag="IT904_001026"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAP3190846.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            24446..24781
                     /gene="arnE"
                     /locus_tag="IT904_001027"
     CDS             24446..24781
                     /gene="arnE"
                     /locus_tag="IT904_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAP3190847.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24781..25167
                     /gene="arnF"
                     /locus_tag="IT904_001028"
     CDS             24781..25167
                     /gene="arnF"
                     /locus_tag="IT904_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAP3190848.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(25161..25427)
                     /gene="pmrD"
                     /locus_tag="IT904_001029"
     CDS             complement(25161..25427)
                     /gene="pmrD"
                     /locus_tag="IT904_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAP3190849.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(25537..26892)
                     /gene="menE"
                     /locus_tag="IT904_001030"
     CDS             complement(25537..26892)
                     /gene="menE"
                     /locus_tag="IT904_001030"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAP3190850.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26889..27851)
                     /gene="menC"
                     /locus_tag="IT904_001031"
     CDS             complement(26889..27851)
                     /gene="menC"
                     /locus_tag="IT904_001031"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAP3190851.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(27851..28708)
                     /gene="menB"
                     /locus_tag="IT904_001032"
     CDS             complement(27851..28708)
                     /gene="menB"
                     /locus_tag="IT904_001032"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAP3190852.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28723..29481)
                     /gene="menH"
                     /locus_tag="IT904_001033"
     CDS             complement(28723..29481)
                     /gene="menH"
                     /locus_tag="IT904_001033"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAP3190853.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29478..31148)
                     /gene="menD"
                     /locus_tag="IT904_001034"
     CDS             complement(29478..31148)
                     /gene="menD"
                     /locus_tag="IT904_001034"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAP3190854.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31237..32532)
                     /gene="menF"
                     /locus_tag="IT904_001035"
     CDS             complement(31237..32532)
                     /gene="menF"
                     /locus_tag="IT904_001035"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAP3190855.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32611..32916)
                     /gene="elaB"
                     /locus_tag="IT904_001036"
     CDS             complement(32611..32916)
                     /gene="elaB"
                     /locus_tag="IT904_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAP3190856.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32971..33432)
                     /locus_tag="IT904_001037"
     CDS             complement(32971..33432)
                     /locus_tag="IT904_001037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP3190857.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33497..34414
                     /gene="rbn"
                     /locus_tag="IT904_001038"
     CDS             33497..34414
                     /gene="rbn"
                     /locus_tag="IT904_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAP3190858.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34602..35820
                     /gene="elaD"
                     /locus_tag="IT904_001039"
                     /pseudo
     CDS             34602..35820
                     /gene="elaD"
                     /locus_tag="IT904_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            complement(35891..>37589)
                     /locus_tag="IT904_001040"
     CDS             complement(35891..>37589)
                     /locus_tag="IT904_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP3190859.1"
                     /translation="MSSLILSGCGPQPENKESQQQQPSTPTEQQVLAAQQAAIKEAEQ
                     SAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGTARYQQFDDNP
                     VKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYFPSDWDIKDKQ
                     SIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLVFLIDTSGSMI
                     SDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDSLDA
                     EGSTNGGAGLELAYQQATKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQRESG
                     VTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQMLITVAKDVK
                     AQIEFNPAWVTEYRQIGYEKRQLRVEHFNNDNVDAGDIGAGKHITLLFELTLNGQKAS
                     IDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPSEDMRFRA
                     AVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADGVTDISQ"
BASE COUNT         9273 a        10024 c         9179 g         9113 t
ORIGIN      
        1 aacggtaaaa ctgtgaccgt tagcctgcgc gagctgaaga aaaaactgta tctctgcctg
       61 atgagcgtta acgcgctgga agcgattcgt ttctacgtca gctttgcttg ttccttcgca
      121 tttgcagaac gcgaattgat ggaaggcaac gccaaaatta ttcgcctgat tgcccgcgac
      181 gaagccctgc acctgaccgg cacccagcat atgctgaatc tgctgcgcag cggcgcggac
      241 gatcctgaga tggcggaaat tgccgaagag tgtaagcagg agtgctatga cctgtttgtt
      301 caggcagctc aacaggagaa agactgggcg gattatctgt tccgcgacgg ttcgatgatt
      361 ggtctgaata aagacattct ctgccagtac gttgaataca tcaccaatat ccgtatgcag
      421 gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg gatcaacact
      481 tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag ttcttatctg
      541 gtcgggcaga ttgactcgga agtggacacc gacgatttga gtaacttcca gctctgatgg
      601 cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa cacccttccc
      661 ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgtcgc gaaggttact
      721 gcggctcctg tcgcacacgc ctggttgcag gtcaagttga ctggattgcc gaaccgttag
      781 cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc gatattgaaa
      841 tcgagatgtg aattggttgt agtgccagat acaacgctta tgcgtcttat ctggcctaca
      901 acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgctttg gggattggct
      961 ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga cgcaagcggc
     1021 gacgactttt ctccaggtca agaaaaccag cttctgcatt accttctgtt ttcacataga
     1081 tatggcgaac ataacaacag ccatgctgac gattaatgct atgcattttc ttaaacgcca
     1141 gcgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat actgcatgct
     1201 gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc agcaacgctc
     1261 gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg atgacaccgg
     1321 cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg ccgaacggat
     1381 aacgtacgga atgaaacaga tgatgcgtca tgcgctttac atacagcttt ttgccgttgc
     1441 gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct tcaacccagt
     1501 cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact gccataccct
     1561 atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg aaaatcttaa
     1621 tgaaacgtgt cgtattaatg aatttctttt gccactacta gcttgacacc gcttttaccc
     1681 ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc cagcagataa
     1741 aatgtgaatt taatcaggcg gatacagcga tgaattttat tcgacaggga ttaggcatcg
     1801 ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc gttcctctcg
     1861 aaccaacttt ctatcgacag atttcgttgc tggctaaaga aaagccggta gaaggcagtc
     1921 cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa atttgatatg
     1981 agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta agaaatttta
     2041 ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta tacagagcat
     2101 catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc acggtataag
     2161 gatgcactac cagtttattc tgctgagcat cttgcaccat gccagtgagt ttgatattac
     2221 ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca tctgcatatt
     2281 ccgccacctg tttcatggca cccggcttaa acatccagtc gtagttgtaa ttaacccagc
     2341 ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc agctgtacca
     2401 gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc agctcatcag
     2461 catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat ttcttcagca
     2521 cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac ggcgctttga
     2581 tttctggata aataccgata tttttcccgg tagagtgatt taacccctga acaaattcaa
     2641 tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa cgccccggat
     2701 aagtctgcac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc aacgacttaa
     2761 tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc cgatccggga
     2821 aacgatcggc aacatcagta acacgatcga ggtaatggtc atgcagaaca accagattgt
     2881 cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca tacgccatcg
     2941 cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg gcgatgacta
     3001 ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg ctcatcatga
     3061 tcgccatgct aaggtttttc agcgtcaatt tcatgccatt agcctccgtt gcgtttttgc
     3121 agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa gataaccgcc
     3181 agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa gtccacggtg
     3241 tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag cccggtaaag
     3301 cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag accgatcagc
     3361 atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc gacggttggg
     3421 ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt cataaagaaa
     3481 acgccggttg ccccacggtt gccacggaag actttatccg acatccagcc gcacagcaga
     3541 gtgcccggaa tacctgcata ttcataaagg aagtaggccc aggaggattt atccagcgcg
     3601 aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta acgcagcaga
     3661 taaacgaaca cgttggcgat ggcgatatac cacagcagtt tgttcggcag tacgtactgc
     3721 atgaagattt gcttcgccgt cagctcctgt tccgcttttt cgttatagtc gtccggataa
     3781 tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc gcgcatcatc
     3841 gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag cgccgcatgc
     3901 cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc accaccgacg
     3961 ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga ccaccagtgc
     4021 accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca gaggaacaac
     4081 agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat aaacaacatc
     4141 actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga gcgatccgat
     4201 accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga aagggcaaaa
     4261 cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag cgcaaagttc
     4321 ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat ttgccagcgc
     4381 aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg tggcgctggt
     4441 ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg attaacagcc
     4501 tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa aacattcata
     4561 aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt tcaaaatgac
     4621 gcatgaaatc acgtttcact ttcgaattat gagcgaatat gcgcgaaatc aaacaattca
     4681 tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa tgaaaactcg
     4741 cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag ccgggattgc
     4801 ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg acatcgcaac
     4861 cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg cggtaaccga
     4921 tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca ttgcacgtca
     4981 ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc tctccttcca
     5041 ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta tagacccgca
     5101 gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg tgaaagttcc
     5161 ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg ccaaagaaca
     5221 cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag gcgcgacggt
     5281 gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc atgcacctgt
     5341 cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg atctgcgcat
     5401 tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta accagcatgt
     5461 gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg ataccatttc
     5521 gctgattggt accacctctt tacgtattga ttacaacgag attgacgata atcgagtgac
     5581 ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg tgatggcgaa
     5641 aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg atgacgaccc
     5701 gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac gcgatggtct
     5761 ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga tggctgaatg
     5821 ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga ctgccgatct
     5881 ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca tctccctgcc
     5941 tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg cctggctgag
     6001 cgaaggccgt ctgcaccgta gcctggtatg tgagtgcgaa gcggtaactg cgggtgaagt
     6061 gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc gtcgtacccg
     6121 tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg gactgctgca
     6181 acgttttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct tccttaacga
     6241 acgctggaaa ggcgtgcaac ccatcgcctg gggagatgca ctgcgcgaaa gcgaatttac
     6301 ccgctgggtt tatcagggat tgtgtggtct ggagaaggag cagaaagatg cgctttgata
     6361 ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg caaaaacacg
     6421 gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca tccggttcgc
     6481 tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac agtggactgg
     6541 aatctttgcg tcagcaggca cctgcacatc cttactccct tctcgggcca caacgcgtgc
     6601 tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa ttgcagggca
     6661 gcgtagaact ggctcaccag cggattacgc cgctcggcac tctgcgctct acctggctaa
     6721 gttcgccaga agtccccgtc tggccgttgc ccgcgaagaa aatatgtgtg gtcgggataa
     6781 gcggcctgat ggattttcag gcgcatcttg cggcggcttc gttgcgcgaa ctggatcttt
     6841 cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat aacgccaccg
     6901 aatttcgcgc tgtgaatatc gcccgtttcc ttgataatga agaaaactgg ccgctgttac
     6961 ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc gcctgcttcg
     7021 gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt tcactgatgc
     7081 ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag ttacagcgcc
     7141 agtttgtgca ccagggtggc gtgtggatgc cgggcgatga agtgaaaaaa gtgacctgta
     7201 aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg ctacgtccac
     7261 gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actggtagcg gaacgtaacg
     7321 gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg ggtgaatggt
     7381 ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc actgatgaga
     7441 cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc ggttcggtgc
     7501 tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc gtcagtgctt
     7561 tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg acaccagctt
     7621 cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc gggtgaatcc
     7681 cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt tgaaagatgg
     7741 cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg aagtcgcctg
     7801 cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcaaaat atgacaccac
     7861 gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta gcgtttccac
     7921 gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc agctgcttga
     7981 tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg gcacgttccg
     8041 tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg tcgctttctt
     8101 tcacggatgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa ttaaagtgct
     8161 caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg gcgtaccgct
     8221 aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg tagagtcgat
     8281 ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa cctgtacatt
     8341 tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct tgcgcgatca
     8401 tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa cgttaccgct
     8461 gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa aaatgggctg
     8521 gacgctctac accctggagc tgttgcgtaa aatcccgggg cttgagttaa cggtgctgga
     8581 ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact accccacctc
     8641 acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag atctggtggt
     8701 caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc gctgcgaaca
     8761 tccgattacg ctactggccc aggcgctggc ttaaactcct ttctgatgcc cggtaagcat
     8821 gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt tgcaacgcag
     8881 tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca aaggcctggg
     8941 ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga ccgaatcaac
     9001 aacctcctcc ccgcatgatg cggtatttaa aacctttatg ttcacacccg aaaccgcacg
     9061 ggattttctc gaaatacatt taccagaacc actgcgcaag cttagcaacc tgcaaacctt
     9121 acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact cggatgtttt
     9181 gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg aacatcaaag
     9241 ctctgcagaa aagaatatgg cttttcggct aatgcgctat gccactgccg ccatgcagcg
     9301 tcacctggat aaaggctatg acagagttcc gctggtggtg ccattgctgt tttatcatgg
     9361 cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg atccgcaact
     9421 tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg tacctgacga
     9481 tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc atattcgcga
     9541 ccgcgattta atcggcatgg tcgacaggat caccacgctt ttggttagag gcttcactaa
     9601 tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata cctcccgttt
     9661 cacccgtttt attgaggaga ttgccgaacg ttcaccacta caaaaggaga gattaatgac
     9721 tattgctgaa cggctacggc aggaagggca tcaaattggc tggcaggaag gtatgcatga
     9781 acaagccatt aaaattgctt tgcgcatgct ggaacagggc tttgatcgtg accaggtgct
     9841 cgcggccacc cagctaagcg aagccgatct ggcagcgaat aaccactaat taacacaggc
     9901 ccacagccga tccccatggg cctttgatat caataactac cttttatgcg tggcccattt
     9961 ttgcctgatt taaacatcgc cagtcgttga tccagggcat cgctgtagag catcgtgtca
    10021 acgccaacag cgacaaagtt cgctccccac gccaggcatt gctgcgccat atcaggagcc
    10081 acagccagaa aaccagccgc tttacccgca gcacggatcc gccgaatact ggtttcaata
    10141 attcgctgca cttccgggtg cccggcgtta tccgggtagc ccaacgacgc agaaagatcc
    10201 gcaggtccaa taaacacgcc atcaatccct tcgacgtcga ggatttcgtc caggttatcc
    10261 agtgccgttt tactttccac ctgcaccaac agacaaagcg aatcgttaac ttgcgccatg
    10321 taattctcaa tgcgtcccca gcgcgcagcc cgtgccacac tggccccgac accacgctca
    10381 ccgtagggag gatagcgcgt ggcagacacc acctgacgtg cctgttcggc agtatcgacc
    10441 atcgggatca gtagagtttg cgcgccaata tccaggactt gtttaatcag cggtttactg
    10501 ccttccaccg gacggatcac gggttggctg gcatagggcg ctaccgcctg tagctgatga
    10561 taaagatcct gaatggtgtt tggcgcgtgc tccccgtcaa tcagcaacca gtcataacca
    10621 gaagtggcgg caatttctgc catataggca gtcgttgagc ttaaccacag accaatttgc
    10681 acttcgccct tgcgtaaacg ttctttaaag ggattgctta ataatgcgtt catcgtgttc
    10741 cttaacctat ttaatgatgt gccacgtcgg tctgcgcaac agtgcgattt acacgcagag
    10801 tgaaaataat cagcgaaccg atgaccgcca ccgctgccag cgtcagtaat cccgccgcat
    10861 cgctggcaaa taacgtttct gctttcacgc gcaggatcgg ggcaataaac ccgcccactg
    10921 caccaaacag gtttacaaaa ccaatttccg cagccagcgc cgtaccggaa agcagttgtg
    10981 tcggcatcgt ccagaacact ggctgcacgg caataaaccc gatagctgca acacacagcg
    11041 ctacgatcgc cattactgga gaaagcagcc cggacagacc aataccaatg cccgccgcca
    11101 gtaatgtcag cgctgcgaca ttacgccgtt caccggtttt atcggaatag cgcggaataa
    11161 gccaggtccc aaacaaggcc gcaacccacg gaatggcggt gaccaccgac gctgtaaagc
    11221 ccacttttgt tcccagcaat gccgcaacct gggtcggcag gaagaaaatt aatccgtaaa
    11281 ccgccacctg aatggtcagg tagataatcg ctagttgcca gactcggcca ttacgcagcg
    11341 catcgctcag ccgagaagtc actttctgtt gttcttcact tgccagttga ttgataagca
    11401 acgttttttc ttgtttactc agaaaacgtg cctgctccgg tgtgtcatca agccaaaaga
    11461 atgtgaatac cccagcaccg actgccaaca atccttcaat cacaaacatc cagaaccagc
    11521 cgggatgccc cataaatcca tgcatctcca acagcgcgcc ggaaagcggt gatcccagtg
    11581 ttaacgccag cggtgcgccc atatagaaca gccccataat gctggcgcga ttacgctgcg
    11641 gaaaccattg cgaggtgaga taaatcatac cagggaaaaa tccggcctcc gcagcaccaa
    11701 gcagagtgcg aactatcaga aatttcgctt cagtatcggc ccatgccatg gctgccgaaa
    11761 gaaatcccca cagcagtgtt gtcgtaccaa tccaggttct ggcccccagt ttgcgcatca
    11821 aaagattcgc cggaacaccc agataacgca tataccacaa agaaaatgcc tgctcccagc
    11881 gcataagctt cattactcaa cccggtatca atctggtagg tctgtttggc aaaaccgata
    11941 ttcgaacggt cgagaaacgc cagcacatac agcgccaaca taaacggaat taaacgcaca
    12001 cggtttttct tcaccacggc gtcaagcaaa gcggtgcaca taaaaagctc cttagaatat
    12061 gtgggcgtta ccgttgtcac agcaacgccc ggagtgatta gtggctgtag gggcgtttca
    12121 aattgcagtc acgattgagt tcgacgccaa aaccgggttt atcaagcact gatttatgaa
    12181 tacgaccatt caccggaacc ggctcattga gcagaatcgg gtcaaactgc ggacgcatcg
    12241 ttgaacaatc cgggctggtc atcaggaatt cgctgaatgg cgtattggtg aaggtgatca
    12301 ccgcatggtg agagtaaaca gacgaaccgt gcggcaccac cagttgcccc ctggatttgg
    12361 cgattgcggc aatttccacc agcgtggtta aaccaccgca ccagccaaca tccggctgca
    12421 taatgtcgat accggtttct gaaagcgtac gaaaagattg cagtgtgccg tggtgctcac
    12481 cgctggtgac catcattccg actggcgcgt tgcgtttcag ttcgcgataa ccttcatact
    12541 gctgtggcgg caggcactct tcgatccatt tcaggttata gggcgcgcaa gcgtgggcca
    12601 gtttggtcgc atagttcacg tcctgactca tccagcagtc gagcattaac cagaaatcct
    12661 caccgcattt ttcacgcata tccgcgacca tagcggcatc tttgcggatc cccgcatcgc
    12721 catcatgtgg cccccagtgc gtcggcattt tgccaccgat aaagcccatc tcttttgcca
    12781 gatccggacg cgcacctgtg gcgtagaact gaatctcatc acgaacagcg ccgcctaaaa
    12841 gtttataaac cggaagcccg accactttgc cgaacagatc ccacagagcc agatcgacac
    12901 aggaaatcgt attcatcacc aggccaccag agccggagta atacagggtg gcactgagca
    12961 tttgatcgtg gatcagtttg atatcactga cacatttacc ctcaatgaaa cggttaagat
    13021 gtttttcgac aataaaacag cccatttcac cggctgtcga aacggcgaat ccggtctggc
    13081 cgttttctgc ttcgacttca acaaccaacg tgccaagaac gttaatgcca aatgactggc
    13141 gtgactgctc gtaatcgcgg tatttactca tcggggtggc aatatgatcg tctatccagt
    13201 gattcgcccc ctggtcgtga taatcaccgc cgccagcgcc tttttctgct gtcgcaccgc
    13261 cagtaaacca ggcgcgaacc tgtttaattt ttggtagggt catgatgttc tccattgtta
    13321 tgaggcttgt aagtcaaagg gacttttcca tcccaacaga cgtgaaatat ccctggcgca
    13381 ggcaatggcc ttgcccgcca gataatcacg gtattcttca ttgatttgta agcgggtacc
    13441 gaccaccgag atcgcagcgg taagctcgtt attggcgtta aacaccggcg cagcgacaca
    13501 acggacatcg gcgtaatctt cgccgttgtc atagctccag ccctgacggc gaatacgcgc
    13561 cagttcttcg tgaagttgct gtggatgagt aatcgttgtg ggtgtcgcct gctcccagac
    13621 cagcccttcg ataatacttt gctgtaccgc tgcaggttgc caggcaagca ggcatttacc
    13681 tattccggag cgataaagcg aaaggctttt tccttcatgg gaacgcacgc tgatagtggc
    13741 tgatgactcc actttcaaaa tgtaataagc gctgccgtta tcaataatcc ccaggtgaca
    13801 taacagcccg gtggtatcca tcagttgcgt caaacgcggt cgagccagtt cccggagatc
    13861 catcttgctt aacgcatgac cggaaagctc caccagcctg gtccacaaac aaaaattttc
    13921 ctggtgatca aggctaagaa aacgctgacg cctgagttca ttaagcagca aataggccgt
    13981 gcttttaggg attcccaatg tgtcaatgat cgtcgcagca ctacaggggc caatgcgggc
    14041 aatcaaattg agaatatcaa tagcccgtgt cagggcggga actttgcttg attccaacat
    14101 actggactcc agtcttagat actggaatca gtctcgttgc tcagggggag taaaattgtg
    14161 aagcgcatca aatcccgctc ctcgtccagc agactggaga aattacgcac aaatttgtga
    14221 atcagcgcac ggattttttc ttattgagta caacaggttg gacaaatttt gccggagggg
    14281 gaatgtgccg gtgcagtaaa ccggcacaga gtggcggaat cagacagata aggtcatgga
    14341 ctcaacaacc tcaatccagc catgctcact ggcgatatcc tggccgttta accaacggcg
    14401 cagcatattc agtgccatca ttgcgcacac ttcctgacgg atagccaggc tgtagcgcgt
    14461 agtgctgaaa cgcacacgca gagcgaaagt gccgtctggc gtggctagcg caaagttgag
    14521 atgctcgttc tcgaaacccg aaacagccag tgccagcccg gcaaaatggt tggcccgccg
    14581 ttctgtaatc cagtgcgcag tttgcgccag ggtttcctcc tgtgaaggaa ccacttcaca
    14641 cgccagcaat ggagcacctg cgcgagaaag ttgcaaagcc aataaaccac cggtgaattg
    14701 ctcgctcaac gtcaggctga actggcggtt ttgcaattcg cgactgatct gcgcgggcag
    14761 tccttcagtg ccttcgaaaa tcacgctctg tccggcaaca cgtttaacat ccagccacag
    14821 tttttccatc gcctgttgct cgcttgccgg tccggtgagt ttcagttcga tgataggcat
    14881 tgaggagcga tagcccattg ttacgcccgg cggcagttgt agagtgtcca ggctttgtgc
    14941 cagatcgctt tccgaacgac caaaagtagt caaacgcaga caaaccggcg gctgcggtaa
    15001 agaaaaacgc tcgcgcaggc gcggcaggat ttcgtgctcg accatcacct taaattctga
    15061 cggtacgccg ggagtaaaga acatcaggca acgattaagc tgcacggcaa aaccacaggc
    15121 ggtgcctacc gggttattga taaattcagc actggcaggc agctccgctt gtttacggtt
    15181 gctcggtgcc attactcgtc cacgttcgtg gaaatagcgt tccatctctt tgagccaggc
    15241 ttcatgcagc accaggcctt caccttttgc tgtcgcagcg gcgagtgcgc ttaaatcatc
    15301 gctggtcggt cccagcccgc cgttaacgat cagcacatcg gcgtgctgac tacgttcgcg
    15361 aagaatggtg actaagtcat caaggttatc ccccaccgta ttgcggcgag ataatggcaa
    15421 cccctgatga aagaaaaaat cggccagcca ggcagcgtta gtgtcaacga tttgcccgtg
    15481 taacacttca tccccggtgg ataacatttc cacttttaac atggttatct cctgcttttg
    15541 ttatcgaatc actataacgc aaagggagat aaggcgtagt aagaaactga atggcgcgac
    15601 agaatgccgc gccggaaggg atcagaaact ggcgttaacc ccaacatacg ggccgtcagt
    15661 cacggcgttg tcgcggttac cgtctttacc cgacagattc aggtagcgat aacccgcctc
    15721 aatactgact ggacgcataa tggtgtaacg cgcgccagca ttcgcttctt cataactttg
    15781 aataccgctg gagagcgaat ccggagagta gtaatactcg ccaaacaaac ggaagctgtt
    15841 gccaattttc cactacaaac cacctcctac cgctgcggca taaccttcat cgccgtaatt
    15901 cgggttggtg taaacgcctt ttccgccaac ggtcgccatt aacggcccga gaggcagatt
    15961 caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg ttatgtgtcc agttaccgct
    16021 taaagccagg cccgtcgatt cagttccaaa accgacacca atattggtgt attctttacc
    16081 cgcctggccg ctgatgctca ttgcattgac cgatgcagaa accaacagca ttccggccag
    16141 acctgctagc gcaatttttt tcattatccc ctttcccaca agcaattaat caaaattccc
    16201 aaagctgcgg gattgtactg cctggcacag aggattcaat gagcgaaaaa agggatctag
    16261 tcacttattg taactaaata cttcttaaat aattaaacgc cgaaaaaata aacacacttt
    16321 accgatacac acaaaaataa taataagtac agttatcatc gcaaaagaga ttactgtcac
    16381 tttcacatat ttgtgagtta ccgtattctc atggtttctt tttatttata aggaatatcc
    16441 ctgtgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct tatttgctgt
    16501 gtaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg ggtggcggcg
    16561 tagagcctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa gaactgggag
    16621 aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt cgcaccaaga
    16681 cgtatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt gactgcgttt
    16741 ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg gtaaaacctg
    16801 aagatctggt gcactatgat ttgaacgttg ccacccgaaa aacgttacgt ttgaaaggtc
    16861 ttctgtaaca atgatgacag ccattactgg ctgtcagatt gaaaatttag tggttaacga
    16921 attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca ttcagataat
    16981 caggtttaaa tgtcgcatca cgcttatctt tagcaatata tgtcaggcaa tgattatggg
    17041 taaaaataac gatattttta tcaggtgctt tgctttgtaa gtctttaatt gcactataaa
    17101 tatcattacc gcactgaaga aatcgtttat ctaccgtcag ctttttaccc gctgaaaacc
    17161 aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa tcagggatat
    17221 cagcactaaa agcgttgccc agttcacggg catcctgggt acctttaacc gtaatacctg
    17281 ttatatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg cgaaacaaaa
    17341 cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata cgtggcaaac
    17401 cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc gccagtaaaa
    17461 tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg taaactccac
    17521 ctatagacaa gcgcaaccag acaattaccg tgaaattgag cgacatttct ggcgataatt
    17581 agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa aattttgcca
    17641 aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct aatattagtt
    17701 tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac
    17761 aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt
    17821 cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag
    17881 aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct
    17941 tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc
    18001 atatcacgct aatggcgttg aaaattggca agggcgatga agtgattacg ccttccctga
    18061 cttgggtttc aaccctcaat atgatttcct tgttgggtgc aacgccggta atggtggatg
    18121 tcgaccgcga tacgctgatg gtcacgcctg aagctatcga gtcagccatt acgccacgca
    18181 ctaaagccat cattccggtg cattatgccg gtgcgccagc agacattgac gccattcgcg
    18241 ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt
    18301 attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcgttt catgccatca
    18361 aaaatattac ctgtgcggaa ggtggcctga ttgttactga taatgaaaat cttgcccgcc
    18421 agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg
    18481 gtcgtgcacc gcaggctgaa gtattaacgc cgggctataa gtacaatctg accgatatta
    18541 acgccgcgat tgccctgaca cagttagtca aattagagca cctcaacacc cgtcggcgcg
    18601 aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc
    18661 ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt
    18721 gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt
    18781 tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt
    18841 taccgaatac cgaatggaat agcgaacgca tctgttcttt gccgctgttc ccggatatga
    18901 ctaccgccga tgccgaccat gtcatcacag cccttcagca actcgcagga caataagcca
    18961 tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg
    19021 aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt
    19081 atgagatcct gctgattgat gacggcagta gcgataattc cgcgcatatg ctggtcgaag
    19141 cctcacaagc ggagaacagc catattgtgt ctattttgct taaccgcaat tacgggcaac
    19201 attcagcgat tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg
    19261 ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt
    19321 acgacgtggt tgggactgta cgccagaacc gccaggacag ctggtttcgt aaaaccgctt
    19381 cgaagatgat taaccggctt attcagcgca ccactggcaa agcgatgggt gactacggtt
    19441 gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgca
    19501 gcacctttat cccgattctg gcgaatatct tcgcccgccg tgcaattgaa attccagtac
    19561 atcatgccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga
    19621 tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggta
    19681 gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct
    19741 tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta
    19801 ttggcgctca gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg
    19861 atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa
    19921 atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc
    19981 tctgctggct gccggttacg aaattagcgc catttttacc cataccgata atcccggtga
    20041 aaaagccttt tatggttcgg tggctcatct ggcggcggaa agaggcattc cggtttatgc
    20101 gccggataac gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt
    20161 gattttctct ttttattatc gccatcttat ttgcgacgag attttgcagc tcgctcccgc
    20221 aggtgcattt aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa
    20281 ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaaacg
    20341 tgccgatgct ggggccattg tggcgcaact gcgcgttgcc attgcgccag acgatatcgc
    20401 tattacgctg catcataaat tgtgccatgc cgcgcgccag ctactggaac agacattacc
    20461 cgccattaaa cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt
    20521 tggtcgcaga acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca
    20581 caacatggta cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca
    20641 gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag
    20701 cgtgatttct attgcaccgc tgttgattgc ctgtggcgat ggcgcgctgg aaatcgtcac
    20761 cggacaggcg ggcgacggca ttactatgca gggctcgcaa ttagcgcaga cgctgggcct
    20821 ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccggat
    20881 actcatcctc ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga
    20941 agatcattat gaagtttacg gtctggatat tggcagcgat gcgataagcc gttttctgaa
    21001 tcatccgcat tttcactttg ttgaaggcga tatcagtatt cattccgaat ggattgagta
    21061 tcatgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata
    21121 tacccgcaac ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg
    21181 ctactgcgtg aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat
    21241 gtgtagcgat aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa
    21301 accacgctgg atttattcgg tatcaaaaca attacttgat cgggtgatct gggcctatgg
    21361 cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt aactggatgg gaccacgact
    21421 ggataacctt aatgcggcac gaatcggcag ctcccgcgct attacgcaac tcattctcaa
    21481 tctggtagaa ggttcaccga ttaagctgat tgatggcgga aaacaaaaac gctgctttac
    21541 tgatattcgc gatggtatcg aggcgttata ccgcattatc gaaaatgcgg gaaatcgctg
    21601 cgacggtgaa attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg
    21661 cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc
    21721 gggctttcgc gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg acgtagagca
    21781 tcgtaaaccg agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat
    21841 gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa
    21901 accatcatga ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa
    21961 ggcgtgccgc gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc
    22021 agcgtcggcc cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg
    22081 tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct gggatatttt actggcaggt
    22141 acggcctggc caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct
    22201 aaacatcacg aagtcggcct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt
    22261 aactgggatc ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag
    22321 attatcggtc aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg
    22381 atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg
    22441 ttccgtccat tgctcgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc
    22501 acctgggatg aagtgattgg tcgggatgtg aaagcagaag attttaacgg ttggttactc
    22561 aaccgcatcc tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc
    22621 tgcgcttatc agcataattt tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca
    22681 ttttgccctt taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga
    22741 aatattgccg gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga
    22801 aatcggtacg ttaccttatc ggcctcttcg cgtttattgc ctgctattac ctgttaccga
    22861 tcagcacgcg tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa
    22921 tgctggcatc cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac
    22981 ccattgccgg atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg
    23041 gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta
    23101 ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat
    23161 tgtttattgt ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc
    23221 tggtggcggg aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa
    23281 gcgcaggatt tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc
    23341 tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga
    23401 aagatctctt tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctccctt
    23461 ggggactggc gatagcgcag cgggagccta acttctggca ctattttttc tgggttgagc
    23521 atattcaacg ctttgcactg gatgatgccc aacatagagc tccgttctgg tactacgtgc
    23581 cggtcatcat tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag
    23641 gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc
    23701 tgctgttttt ctccgtcgct aaaggtaaat tgcccaccta tattctttcc tgctttgcat
    23761 ctctggcaat gctgatggcg cattacgctt tgctggcagc aaaaaataat cctctggcgc
    23821 tgcggattaa tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg
    23881 tggtctcccc gtggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag
    23941 tcttttgtgc ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa
    24001 acgtcgaaaa gacctggcct tttgccgcgc tttgcccgct ggggctggcg ttgctggtag
    24061 gattttcaat tcctgacaga gttatggaag gaaaacatcc gcaatttttt gtcgagatga
    24121 cacaagaatc acttcagcca agccgctata ttcttactga tagcgtcggt gttgccgcag
    24181 gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga
    24241 aatacggcct taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa
    24301 actggcttaa tcaacatcgt caggagggga ttattactct cgtgctttcg gttgaccgcg
    24361 atgaagatat caacagtctc gccattccgc ccgcagatgc catcgatcgt caggagcgtc
    24421 tggtgctgat tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct
    24481 tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg
    24541 gcgcaaacat atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatggt
    24601 gctctggctg ctggtcttgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct
    24661 gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg
    24721 tcactggtgt ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta
    24781 atgggcctga tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg
    24841 ggttttgcgg caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg
    24901 ttagcttttg gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc
    24961 gttttttgtt ggtataaaac gcttcataaa cttgcgctaa gcaaagccta tgccttgctc
    25021 agtatgagtt atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact
    25081 ttttcgctga aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc
    25141 ctgcccatga caaaacaacg ttactgagtt ttccctgcca ctttacactg ccgttcccac
    25201 tcatcggggc tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta
    25261 atacaataaa gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca
    25321 ctcttcactt cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt
    25381 ctgttatctt gtttattgca gcacgatttt ttgaccagcc attccattgc attatcctgt
    25441 ttgctaagag ttttcacatc aatttacaga ttaacagata aactctttat caacggcaat
    25501 agaatggcag aaatgagaag tgaaacggag ggttaattat tgctgacgtt gcacccactc
    25561 ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc aattcaggcg gcaacgttaa
    25621 ccaacgcacc ggttgctgaa aacgggccag cttatctttc acccattcac taagatcaac
    25681 gctctcgtgg tcatactcca tcaccgccac cggtcgatga ccaaactcct tgtcggcaac
    25741 ggggacgata aacacctgca aaaccgcagg atgtgcagca attacgcgct cgacttcttc
    25801 cggctgaata ccctctccgc cactgaagaa tagattgtct aaacgtccga caatggtcag
    25861 cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt cattaaccag
    25921 tgaaaccagt tgcccgttac gccagtaacc ttctgccata ctggcagccc gcagccacac
    25981 ttcattatta acgattttca cttcccgacc cggcagcggc gaaccaacgt ctgccaggcc
    26041 gtcggcttct ttcgcacaca ccgtggaggc aaactcggtc agaccatagc cgcaaaagca
    26101 acgaatcccc tgctcgcgcg cctgttccgt caactcgacc gggatagccg cgccgccaag
    26161 taacaccgct ttcagggaaa cggaactgcg gttaaccagc aaacgccaga gttgtgttgg
    26221 caccagtgaa gcatgagtac agcctgccag catttgctcc aatggctgtt tatcacgcac
    26281 cgtcatccgc gcaccagcgt ataaccagcg ccacataatt ccctgaccgg agacgtgaaa
    26341 cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac ggaatcaacg ataacacccc
    26401 ttcggcactg gcaagatggg cctgataagt atggacagcg gctttcggca acccggtaga
    26461 acccgaggtc aacgtcattg agcacagacg cgtcggctgc cacgtagcgg catgtgcgcc
    26521 ttcaacccgc tgaatgtgca gcgacgttaa cgccggaaac gtgttttccc catccggcac
    26581 cagcgcaaat tgcagcgtca gattgggcag caattcttca agcaacggtt gcggcagctg
    26641 agggttcacg ggcaacaccc gcgccccgca ttgcagtaac gccagccagg cgagcagcgt
    26701 ttgcggcgta ttccacgccc gcaacatcac gccgctgccc tcaacaacgc cctgcgccgc
    26761 aaagcccgta gctaactcat cgacgcgagc acaaagctcg cgccagttaa gttgctcgtc
    26821 attaagacgt aaggcgatgg cttctccccg cacttgccgc cagtgacgcc acggccagtc
    26881 agagaagatc ataacaaccg ctccagtgca tcaacttcca cgacaggcag cgtgctaccc
    26941 ggccagcgac gtacctgctg cgcctgcatc agatccagcg tgtccagccc tggaatggtg
    27001 tccggcgtta accaggcggc aatccgcgcc agttgcgtta agcctaagct cgattcaatg
    27061 gaagaactga tcaccgccgt cagccccagc gcgtgcgccg cctgtacctg ctcgcgtact
    27121 ttctccagac tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc ctcttcagcc
    27181 acaaaggcaa aatccggctc gcgcaagctt tcatcccagg caatggcaat gccggtttca
    27241 cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa cgcgatgcgg
    27301 tggcgataat ccgggttaac atatttggca aactgctggc ctttcagcgg tgtccaggcg
    27361 cgatttgcgt caagacgcaa atgcagatcc ggaattgcct ccagcaacag attaaccacc
    27421 atgccgtcgc gcacggcttc gtacaatccc actttgacct tcgccacttt ctcgcctggc
    27481 atatcagcaa gtttgaggat cagatcgtca ggatcgccat tacacagtgg tgccgcacgg
    27541 taattggccg cctgcgggag cgtatctgcc agctccgcca atgcacagct tacgccaaag
    27601 gcaacggaag tcatctgcgg tagctcgcaa tcgcctgcca gccagttatt tacccaggca
    27661 agcagcacgc tttgcgcctc ttcccaggtt tcctggctga agcccggcag tggggagatc
    27721 tccccccacc cttcgcgctc gccctcacgc aggcaaacgt acagcccgtc gcgggttttt
    27781 aaccgcctgt cgcgcagaac cacccccgcg tccatgggga tctgccagcg gtatacctgc
    27841 gcactacgca ttacggattc cgtttaaatt tgctgaaatc aggctgacgt ttctggttga
    27901 aagcgttgcg accttcctga ccttcttccg tcatgtagaa cagcatggtg gcgttgcccg
    27961 ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt cagcgcagct ttcaggcagc
    28021 gcagcgccat cgggctgttt tgcagcattt cgcggcacca acggacggtt tctttttcca
    28081 gatccgccag cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt tttgcgtcgt
    28141 actgacggca caggaaccag atttctcgcg cttttttctg cccgacgata cgagccatgt
    28201 aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg gccagtctga ccgaagatgg
    28261 cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag aacgtgacca ccgccgatgg
    28321 agtagccagc caccatcgcg acaaccggtt tcggacaggt acggatctga cgctggaagt
    28381 ccagcacgtt caagtgatgt acgccggaat catctttata gccgccgtaa tcaccacgca
    28441 ctttctggtc accaccggag cagaacgctt tatcgcctgc gccagtcaga atgatcacgc
    28501 cgatattgtc gtcataacgc gcatctgcca gtgcctggat catctctttt accgtcagag
    28561 gacggaaggc attgcgcacc tgcggacgat taatggtgat ttttgcgata ccgtcggtgg
    28621 atttttcata acgaatgtcc tcgaaacctt cggagcagtc gtgccattca accggtgcgt
    28681 aaagcattgc ttcatcagga taaatcatat tgtgtccttt agtcagaaac gcaagatctg
    28741 cgccagactt gcgattacgc cagcgggatt ttcccgatgc gcgttatgtc cggcgcgagg
    28801 aatgacatgg cagtcggcag ccagttccgc cgccagggcg cggaatttgc tgtcacgttc
    28861 accacataaa taataaaacg caaatgtgcg ggcgctaagg ttagcacgta aatcaggctg
    28921 gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt gcgccattat tgttgctgcg
    28981 cagcaccacc agctcccggc gttgatcgtc attgagcgag gcaaaaacag gctgttgata
    29041 ccagtcggca aataccgccg ttaacggttc tgtgcaaaaa cgctgcgccc attggcgatc
    29101 ggaacgctga cgttccgcac gttgttcagc attttgcagc cccggatgcc cgccttcgac
    29161 aataacccca caaagccccg tcagcccctg gcaagccgcc atcatcgcca cccgtccgcc
    29221 aagcgagtac cccaccagcc agaagtcaag gatgttgtaa ctaaccaagg ttttacgcag
    29281 taagtcggtg acatcatcaa atccatcgac gctaatcgcc gccgaaccac catgacctgg
    29341 gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg cccacttctt gccattcgtg
    29401 gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt ttccgtgttt
    29461 tgcctgcgcg tgcaggatca taaatggctt acctgcgcca gaagttgctg gagcgtttgc
    29521 gcaccatcgg tgtcgttaac caccatttca atcaccgtgg tggttggcgt gcgccaggcg
    29581 tcggcaaatg ccgtttcaag ttcctgccag ttttgcggac gatgatattt cagctcgaac
    29641 atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca gatagaaacg ctcacgctcg
    29701 ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac cacaattaat
    29761 accagcggcg cagaaacctg acgcaataac gccagcgcgt tgagatcgta aagtgcggag
    29821 agatcgccca caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc tgcggcggtc
    29881 gaaagcagtc cgtcgatacc gctggcccct cggttgctgt acaccgggta acctgccgga
    29941 agttgcgaaa gcgcatcaat cagacgtacc accaggctgt taccaacaaa caattgcccc
    30001 tgttcaggca gatagtcgca gatgcgatgc gccagttgcg cttcgccaaa cgcatcacgg
    30061 cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc
    30121 tggcgttttt ctgccggatg cagctccagc cagtcggcaa tattggcaat taagcgacgt
    30181 ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat caacaatcca gtactcttct
    30241 ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg tcaggctgct tcccagttgc
    30301 accacaattt gcgcctgctg cagctcgctg gtcgctttgg cattgcctaa ccaaagatcg
    30361 gcacacggca gcggctgccc ggtttgtgac agcacatcgc caatcagcgg ccagccaaga
    30421 gtttgcgccc acagggcaac ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc
    30481 acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc cagacgaggc
    30541 gcttcacgca gccacggttt gtcgtcctgc caccagtcac ccagacgctg ttgccagcta
    30601 agcccggtat cgtccatttc gccatacagc ggttcagcaa acgggcagtt gatatggact
    30661 ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag aaaccagcca acgtgcgggg
    30721 atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc gaacattccc
    30781 ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct ccggcgggcg atcggcggtt
    30841 aagagaatca gtttttctcc ggttaacccg gcttcaatca gtgccggata gagatttgcc
    30901 accgccgtgc cggaggtcac aatcaccgcc accggctgct tgctgacttt cgccagcccc
    30961 agcgccagat gccccaaccc acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc
    31021 tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg gggcgataca gatgtgtctg
    31081 acgccgtgac gcgttaatgc ttccagaatg accgccgccc agcgtcggtt aaatgcgctt
    31141 actgacatga gtttgtccgg tatcaatatt gcggctaagt ataaggagct acaaaaatag
    31201 attattgata tgaatcggta atgatgcgac tcattactat tccatttgta ataaagtacg
    31261 cagccctgcc gctttgttgt cgatttcctg ccactcttgc tcggggtcgg aaccacggac
    31321 aatgcccgcg ccagcatata atcgcacgac attgccgcta atttttgctg agcgcaggga
    31381 aacgcagaat tcgctttgtt gtaatgagag atagcccgca gaaccggcgt accattctcg
    31441 ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta agccagcaac
    31501 tgctgccgtt ggctgcaact gatgtaaaca gatcacatca tccgctttgt tgagtgaagt
    31561 ccagatacag cggcgaagat gctgcacttt acgcagacgc agtacctgcg gcggtaaaac
    31621 atccagcgtc tgggtatcgg cctgtaatcg ttggcagata tcttccacca ccagcatgtt
    31681 ctcgcgctgg tttttatcat ccgccatcag ccactctcct aactgctgcg cctgcttatc
    31741 atcaggatta tttgctactg ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg
    31801 ccgccataac cgttccggtg aagagccaag aaaagcattt tcgccatcaa aggccatgta
    31861 aaaatggtag caattcagat tcagtcgacg actggcagcc atcatcgccg ccgcgttgac
    31921 cggacttgcg aaatgcaggt cagttgcccg agcgagcacc actttgtcga gctcaccttc
    31981 ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt ccggccagtg
    32041 ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc ttgatactca ccagtgtggc
    32101 gataaattct tttgcctgaa tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag
    32161 ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg cggggtaaaa gtaaattgcc
    32221 ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag tcggcgtgtt ccgggtgctg
    32281 gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacgac
    32341 agcttcttca tcaccattac gttgttgcca gtagaattgc gggtatgttt gctgactggc
    32401 cagccagctc aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga taacccgaat
    32461 gccgggtgtc gccggaatct cttgcgacaa atggcgcagt agattttcca gcgccgtagt
    32521 aagtgattgc acgcggacct ctccctgtta aaagcctcac attatacggg gtactacaaa
    32581 aaaaatgcag taccccggtg tagggaggtt ttaacggcgt gccagcaaca gtcctagtac
    32641 cagcccaacg gccgcgccca caccaattcc ttgccagggt ttttcgtgga cgtagtcatc
    32701 agcacgataa actgcctgct tcgcccgata gtaataactg tctgaagcct ggctaacccg
    32761 ttttttcaca tcatccagcg ctttttctgc acgcgctttc agctcaacat atttctgatc
    32821 ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc
    32881 gtcatcgata cgtgtatcac caaactgatt agacattctc gttctccatt tgcgtaaaac
    32941 ctgtcactaa atatagacaa gggttgttga ttacgcctga attacctcgc gtgccatccc
    33001 aatgtgtggg ataccatctt cttcatagac ctccgtcaca gggataaaac cgaaactctg
    33061 gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag gccagtgatg
    33121 cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc actttttcgc cacgcaacgc
    33181 ttcactgaca atcacccgac ctataacgac cggctcaaga tcatcatcac ttttcagaat
    33241 cctcgcatat gccaccagtt catcattttt ccaaccgaga atatggcgat tatcccccgt
    33301 caaatcatca ccatcgatat cctgataagg gcaattctgc tcgacaacaa acaccgcgca
    33361 acgcagttgt aataaggcat aaagctggga aaccgataat tcagagtgat gcagatcttg
    33421 ccattcaatc ataatgctct ccttttagat gtatgcctta tactagccgg aatatttttt
    33481 gaaacgtgat gaactcatgg aattaatttt tttaggtact tcagccggtg tgccaacccg
    33541 cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat ccgacccaga gcggactttg
    33601 gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct ttaaccctgg
    33661 aaaactggac aagattttta tcagtcacct tcatggcgat catctttttg gtttacccgg
    33721 cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt atggtccgca
    33781 aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga ccgattatcc
    33841 gctggaaatt gtcgaaattg gcgctggcga aattctcgat gatggcctgc gcaaagtaac
    33901 cgcttatccg ctggaacacc cactggaatg ttatggctat cgtattgaag aacatgataa
    33961 accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg gcccactgtt
    34021 tcaggaatta aaagcgggca aaacaatcac gctggaagat ggaaggcaga ttaacggcgc
    34081 agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg ataccggccc
    34141 ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct
    34201 ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac gccaggctgc
    34261 gacactagcc cgtgaggctg gagtcggcaa gctaatcatt acccacgtca gctcgcgcta
    34321 tgatgacaaa ggttgtcagc acctgttacg tgaatgcagg tcaattttcc cggcgactga
    34381 actggcgaat gatttcaccg tgtttaacgt ttaactctgc ttattattaa aaacagggtg
    34441 aaacttgccc tgttatagca acccgcgcta catatatcca cccacacagg catattgagc
    34501 gatttttgat atttattcat ctccttacgc actatattca gagcaataag ttgcctcttt
    34561 tcatcttgat tcagcacaac ctttgcaact aaaaggagcc catgatggtt acagttgtca
    34621 gcaattattg tcaattatct caaacccaac tcagtcagac atttgcagaa aaatttactg
    34681 tgaccgagga attactgcag tctttaaaaa aacagcgtta tccggagatg aagaaagtat
    34741 tgagttactg cataatattg cgttaggtta tgatgaattt ggaaaaaaag ctgaagatat
    34801 tctttaccat attgttagaa acccaacaaa tgagacccta tcgattatcc gacttattaa
    34861 aaatgcctgt ttaaaattat ataatttggc acataccgca accaactccc accttaaact
    34921 aactggtccg gataattcag atgttctcct gttcaaaaag ctattctccc cttcgaaatt
    34981 aatgacaatt atcggtgatg aaattcctct tatatctgaa aaacagtcgc tttcaaaggt
    35041 gcttttaaat gatgagaata atgaactgag tgatggtaca aacttctggg ataaaaatcg
    35101 tcaattaacc acagatgaaa tagcttgcta tcttcagaag atcgccgcta atgcaaaaaa
    35161 tactcaagtc aattatccta ctggtctcta cgtcccctac tccaccagaa ctcacctgga
    35221 agacgctctc aatgaaaata ttaagagcga tccatcatgg ccgaatgaag tccagttatt
    35281 ccccataaat actggcggac actggatatt agtttcgcta cagaaaatag taaataaaaa
    35341 aataataaac tacaaataaa atgcgtcata ttcaactcat tgcgtgcact aggccatgat
    35401 aaagaaaatt cacttaagcg tgtcattaat agttttaatt ctgaactcat gggagaaatg
    35461 tcgaataata atataaaagt tcatttaaat gaaccagaga taatattttt acatgccgat
    35521 cttcagcaat acttaagcca aagttgcggt gcgtttgtgt gcatggcagc ccaggaagtg
    35581 attgaacaaa gggaaagcaa ttctgacagc gccccctata cgttattaaa aaactatgct
    35641 gacagattta aaaaatgttc agcagaagag cagtacgaaa ttgattttca acatcgactg
    35701 gtaaacagaa gttgttattt agataaatat ggcgatgcac gaatcaatgc ttcttataca
    35761 caattagaaa taaaacactc acaacccaaa aatagagcat ccggcaaaag agtgagttaa
    35821 catcattgtt aattaattcg accagtcagc aaatatgctg actggtcgaa catagtttgc
    35881 taaacagtca tcactgactg atgtcagtca caccatccgc cagttcaatc aggcgaataa
    35941 attccgccct gtaaccctgt ggatcttccc cttttgcctg ctgagcccac tgtttgatct
    36001 gctgccagga ggtattgttc aggtattcag aaccgcgtaa cttttgccca tatgcagcta
    36061 ctgctgcgcg aaaacgcata tcttcagagg gcgcgtttat tgttggcccc agcgggaatt
    36121 caactaactg actttctttt ccctgcgggt atttccagcg aatttttaac caggccagtt
    36181 ctttcgtttt gtccgatttc gctaatttgt tatccggggc atagcgtaac ttatcaattg
    36241 atgctttttg cccgttcagc gttaattcga ataacaacgt tatatgtttg cctgcgccta
    36301 tatcccctgc atcaacgttg tcgttattaa aatgttccac ccgaagttgg cgcttttcat
    36361 aaccaatctg acggtattcc gttacccacg cggggttaaa ctcaatttgc gctttgacat
    36421 cttttgctac ggtaatcaac atctgccgca tttcactatt caatactttc tgcgcttcag
    36481 agagggtatc aatgtagctg tagttgccgt taccaacatc ggcaattcgc accatcattg
    36541 cctcgttgta attgctattc cccacgccaa acgtcgacag agtaacacca gactcccgct
    36601 gttttttgac cattgattca atcgattttg gatcgtcaat gccaacgtta aagtcaccgt
    36661 cagtggctaa taaaatgcga ttgatgccgc ccttaataaa ccccttcgtc gcctgctgat
    36721 aagccagttc cagcccggca ccgccattgg tactgccttc ggcatccagc gaatcaattg
    36781 cggcattaat ttccgcctta tgactcccgg agatagaagg caatgcaata cgggagtcgc
    36841 cagcgtaggt cacgatggca atgttatcct gctcacgaag ttctttgacc aataatttca
    36901 acgaagactg gataagtggc aaacgttcat cagaaatcat tgaaccagaa gtgtcgataa
    36961 gaaagaccag attagaagct ggtaactctt cacttttgcg atctttcgcc aggatatcaa
    37021 ctttcagcaa tgttcgctgt tcattccatg gtgcaggtgc caattcgtag cgcatagcga
    37081 aaggtattgg cttagaggcc ggaatagatt gtttgtcttt gatatcccaa tcagacggga
    37141 aataattgac tatctcctcc acccgcacag cgtctggcgg aggtaacagc ccttgattga
    37201 ggaaacgcct tacattcgca taactgccag tgtcaacgtc aagactaaac gtcgccaacg
    37261 gattttgcgc tacctgctta accggattat catcgaactg ctggtagcga gcggttcctg
    37321 gatttgcgat atgtgtagct tttgctttag ccgctcttgc aaatgttggc gcttcctgca
    37381 atcgcccctg taaagcctgt ttgtctgaat attgttgcac ttcttgctgg gccaaggctt
    37441 tcgcggcggc ggcgctttgc tcagcctctt ttattgcagc ttgctgcgcg gcaagcactt
    37501 gctgctctgt gggagtactg ggttgttgtt gctgactttc cttattctca ggttgcggcc
    37561 cacatcctga caaaatcaaa ctactcata
//
LOCUS       DACAPB010000023        36213 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.023, whole genome shotgun sequence.
ACCESSION   DACAPB010000023 DACAPB010000000
VERSION     DACAPB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.023"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1833)
                     /gene="malQ"
                     /locus_tag="IT904_001041"
     CDS             complement(<1..1833)
                     /gene="malQ"
                     /locus_tag="IT904_001041"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAP3190860.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLM"
     gene            complement(1843..4236)
                     /gene="malP"
                     /locus_tag="IT904_001042"
     CDS             complement(1843..4236)
                     /gene="malP"
                     /locus_tag="IT904_001042"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAP3190861.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            4848..7553
                     /gene="malT"
                     /locus_tag="IT904_001043"
     CDS             4848..7553
                     /gene="malT"
                     /locus_tag="IT904_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAP3190862.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(7596..8612)
                     /gene="rtcA"
                     /locus_tag="IT904_001044"
     CDS             complement(7596..8612)
                     /gene="rtcA"
                     /locus_tag="IT904_001044"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAP3190863.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     TAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(8616..9842)
                     /locus_tag="IT904_001045"
     CDS             complement(8616..9842)
                     /locus_tag="IT904_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAP3190864.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            10031..11629
                     /locus_tag="IT904_001046"
     CDS             10031..11629
                     /locus_tag="IT904_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAP3190865.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(11611..12369)
                     /locus_tag="IT904_001047"
     CDS             complement(11611..12369)
                     /locus_tag="IT904_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAP3190866.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAQPPVSVMQVLTDHHIQLELC"
     gene            complement(12386..13216)
                     /gene="glpG"
                     /locus_tag="IT904_001048"
     CDS             complement(12386..13216)
                     /gene="glpG"
                     /locus_tag="IT904_001048"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAP3190867.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(13261..13587)
                     /gene="glpE"
                     /locus_tag="IT904_001049"
     CDS             complement(13261..13587)
                     /gene="glpE"
                     /locus_tag="IT904_001049"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAP3190868.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            13777..15282
                     /gene="glpD"
                     /locus_tag="IT904_001050"
     CDS             13777..15282
                     /gene="glpD"
                     /locus_tag="IT904_001050"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAP3190869.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(15498..16100)
                     /locus_tag="IT904_001051"
     CDS             complement(15498..16100)
                     /locus_tag="IT904_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190870.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPMVVALAACIITLSFLGEML
                     FSIPLMIRRISLLQLQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPV
                     YLWLILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLG
                     FALGYYCQWCSKNRCQIRKLSEWGTCLSFVTLGMMAWALR"
     gene            complement(16100..17088)
                     /locus_tag="IT904_001052"
                     /pseudo
     CDS             complement(16100..17088)
                     /locus_tag="IT904_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
     gene            complement(17114..18622)
                     /locus_tag="IT904_001053"
     CDS             complement(17114..18622)
                     /locus_tag="IT904_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP3190871.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIWAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETKVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTME"
     gene            complement(18747..21194)
                     /gene="glgP"
                     /locus_tag="IT904_001054"
     CDS             complement(18747..21194)
                     /gene="glgP"
                     /locus_tag="IT904_001054"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAP3190872.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(21213..22646)
                     /gene="glgA"
                     /locus_tag="IT904_001055"
     CDS             complement(21213..22646)
                     /gene="glgA"
                     /locus_tag="IT904_001055"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAP3190873.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(22646..23941)
                     /gene="glgC"
                     /locus_tag="IT904_001056"
     CDS             complement(22646..23941)
                     /gene="glgC"
                     /locus_tag="IT904_001056"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAP3190874.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(23959..25932)
                     /gene="glgX"
                     /locus_tag="IT904_001057"
     CDS             complement(23959..25932)
                     /gene="glgX"
                     /locus_tag="IT904_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAP3190875.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(25929..28115)
                     /gene="glgB"
                     /locus_tag="IT904_001058"
     CDS             complement(25929..28115)
                     /gene="glgB"
                     /locus_tag="IT904_001058"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAP3190876.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(28388..29491)
                     /gene="asd"
                     /locus_tag="IT904_001059"
     CDS             complement(28388..29491)
                     /gene="asd"
                     /locus_tag="IT904_001059"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP3190877.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            29683..30276
                     /gene="yhgN"
                     /locus_tag="IT904_001060"
     CDS             29683..30276
                     /gene="yhgN"
                     /locus_tag="IT904_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAP3190878.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(30333..31673)
                     /gene="gntU"
                     /locus_tag="IT904_001061"
     CDS             complement(30333..31673)
                     /gene="gntU"
                     /locus_tag="IT904_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP3190879.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(31677..32204)
                     /gene="gntK"
                     /locus_tag="IT904_001062"
     CDS             complement(31677..32204)
                     /gene="gntK"
                     /locus_tag="IT904_001062"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP3190880.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(32343..33338)
                     /locus_tag="IT904_001063"
     CDS             complement(32343..33338)
                     /locus_tag="IT904_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HAP3190881.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(33562..34257)
                     /locus_tag="IT904_001064"
     CDS             complement(33562..34257)
                     /locus_tag="IT904_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP3190882.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(34380..35417)
                     /locus_tag="IT904_001065"
     CDS             complement(34380..35417)
                     /locus_tag="IT904_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP3190883.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFSADDSVGV
                     LEYVNDEGVTVREEIKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            35750..>36213
                     /gene="yhhY"
                     /locus_tag="IT904_001066"
     CDS             35750..>36213
                     /gene="yhhY"
                     /locus_tag="IT904_001066"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP3190884.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDYMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSHWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDA"
BASE COUNT         8837 a         9892 c         8962 g         8522 t
ORIGIN      
        1 catcaacgat gccttatgcc cggcacgttt tggcagacaa ccatatttat gcagtgcatc
       61 cagcagccct tgcttcgcca gttcgcgatc ctgatacaga ccgcgcagta ccacttcatc
      121 cggatacagc cccagggttt tgcccagcgt tagatccccg ctctcccagt agccgcgtag
      181 cgtaggcagg tcatgtgtcg ccgcaaccgc catcgactgc tccggatacg ctttcggtgc
      241 acggaacgtc ttctcgtggt cgttttcgaa atagagcact ttgtaggagt acacaccgct
      301 gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg agatcttcac caatcaccat
      361 acagcgatga cgtttacttt cgagtgccag aatcgaaagc agatcatcca ccggatagtg
      421 aacatacgcg ccctgatctg ccgtctcgcc atacggtatc caccacaaac gcagcatcga
      481 catcacatgg tcaattcgta atgcgccgca gttttgcata ttggcacgca acagctcgat
      541 aaacggttca taggcacgcg cggtaataat atgcgggtcc attggcggta atccccagtt
      601 ctgccccaac gggccgagga tatccggcgg cgcgccaacc gacgctttca ggcaatacag
      661 ttcacggtca caccaggttt ccgccccacc ttccgctacg ccaaccgcca gatcacgata
      721 caagccaatc ggcatttcat agccctggct tatctcccag caggcggcaa actggctgta
      781 agccagccac tgcaaccaga gataaaaatc aacgtcatca cgatgttctt cgcagaactg
      841 acgcacttct ggtgaatcca cgttctgata catctctggc catgcaggcc agccccagcg
      901 catttcgtcc tctttcactt gctgggcatg tagcgcatca aaggctgcct gccagaacag
      961 gctgtcgcct tgatctgcaa caaactggcg aaacgcggcc atctgctcat catcacgttg
     1021 cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt agggcggtaa ccgtggagta
     1081 atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt tgcgtggtcg gcaactgcca
     1141 ccaggcctga gcctcttcgc taagatggaa atcttcaacg gcgttaacgt cgatataaat
     1201 cacattcagc caacggcgag aagacgggct gtatgggctg gcactctccg gatttgccgg
     1261 atagagcgca tgaatcgggt tcaggccgat gaacgacccg ccacgttttg ccacatccac
     1321 cagcatcgct ttgagatcgc caaaatcccc aataccccag tttttttccg atcgcagcgt
     1381 ataaagctgg acgcaggcac cccacagctt ttgtttattc agcaacgcct gcggttcgta
     1441 acagcgcttc ggggcgacaa tcacccggca atgcgcgcgc tggtcgtcct gggtgagtgt
     1501 cagcgtgtga taaccttccg gcagcttcgt cggtagattg aacgctttgc cccccgttac
     1561 atggcctttg tactgtgttc cttcttcggt ggtcagcagc cagctatatt cgccgctgcc
     1621 ctccaccacc atcggcattt ttttgccgct ggtataaacc atgacattcg ggactggcgt
     1681 taccgccact ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc gtttggtttc
     1741 ggcgctaatc gactgcggtt taccgtgggc attgatgtaa ttggggctaa tccccgccgc
     1801 cagcgcggca ttatccagac gtttgctttc catcgagctt ccttagcgtt ttgcctgcca
     1861 gatacgagcc tgataatcgc gaatagagcg atccgagcta aacataccgc agcgggcggt
     1921 attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg tacagcacat ccacctgctt
     1981 ttgtgcctca acataggctg cgaaatccgc catcaccaga tacggatcgc cgccttgttt
     2041 gccgatactg tgcagcatct ggtcgaaggc atgcttatcg ccgtcgctgt atttaccgct
     2101 ttccagctct ttcaatactg cgtccagcac cttatctttc ttccgccatt tcaccgggtc
     2161 gtagcctttg gccagaattg ccttcacttg ttccacggta tgaccaaaaa taaagatatt
     2221 ttcttcaccg actttctcgg cgatttcaac gttcgcacca tccagcgtac cgacagtaag
     2281 cgcaccattg agcgccagtt tcatattgcc ggtaccggaa gcttctttac ctgcagtcga
     2341 aatttgttcg gagatatccg ccgccgggat cagtttttcc gccgccgaaa cgcaataatc
     2401 cggcaggaac accaccttca acttatcgcc aaccagcgga tcgttgttga tcacgtcagc
     2461 cactttgttg atcgcaaaga taatattctt cgccaggtag tagcccggtg ctgctttcgc
     2521 gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg ttttcacgaa tttctttgta
     2581 caacgccaga atatgcagca gattcaggtg ctggcgtttg tactcatgca gacgtttgat
     2641 ctgaatatcg aaaatcgcct gtggattgat ctcaataccg gtacgaactt tcacaaactc
     2701 cgccagacgg actttattcg cctgcttgat ctcgcgatat tgctgacgga atttcgcatc
     2761 atcagcgaat ttttccagat tgatcagctg atcgagatcg ttagcccact cttttttcag
     2821 tgatttatcc aacagagccg ccagtgccgg gttgcactgt ttgatccagc gacgtggggt
     2881 aataccgttg gtgacgttat ggaatttgtt cggccatagc tggtgatatt ccgggaacag
     2941 atctttcacc accagatccg agtgcagcgc cgcaacaccg ttcaccgcga aaccgccaac
     3001 cacacacagg ttcgccatat gcacttgttt gtcgtgcacc accgccagtt tggcccacac
     3061 tttttcatcg cccggccagg ttttctccac cagcgtttta aagcgagtat taatttcgtt
     3121 aataatctgc atgtggcgcg gcagtaagcc tttcaccagt ttcacatccc agcgttccag
     3181 cgcttctggc atcagggtat ggttggtgta agcgaaagtt ttgctggtaa tggcccaggc
     3241 gtcatcccag ctcatctggt gctcatcgat cagcacgcgc agcagttctg gaatcgcgat
     3301 agttgggtgg gtatcgttca gctgaataac ttcgtaatcc gccagttcgt gcagtttacg
     3361 ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa caggcacact ggaagtactg
     3421 ctgcatcaag cgcagctttt taccggcagt atggttgtcg tttggataga gaactttggt
     3481 cagtttttcc gcattgatgc cctgctgttc ggcacgcagg aaatcaccgt cgttaaattt
     3541 agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga cgcagcggct gcgccacgcc
     3601 attacgatag ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa actccggctc
     3661 ccagcgtccg tctttcgtca ctttaccgcc aatccctacc tgcacatcca gtgcttcgtt
     3721 gtggcggaac cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa cctgtttgcc
     3781 atcgacaaaa gactggcgga acaaaccata ttgatagttc agaccgtaac ccgtcgcaga
     3841 ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt
     3901 acccagcgcc gggtcgatct cttcttccag caggtcagtc agattgatgt cataagcctt
     3961 caacgaatcc tgtacatcct gataccagcc gagattcaac aggttgttgc ccgtcagacg
     4021 accaatcaaa aactccattg agatgtagtt aacatgtcgc tgattcgcca ccggtttggc
     4081 gaatggctga gcacgcagca tttcggccag tgcttcactc actgccagcc accactggcg
     4141 aggagtcatt tcagccgcag aatttaagcc ataacgctgc cactgacgtg aaagcgcttc
     4201 ctgaaattgc ttatcgttaa aaataggttg tgacatagga gttccacttt tcttagattt
     4261 tcaacacaac gttatcgcta gtttgccagg ctcgatgttg accttcctca tcctgcgggg
     4321 gattaggcag ggaggagttg cggggatgag caaggaaatg tgatctcaac cacttaaagc
     4381 tagtgcaaac cacaggatta gcagcaaatc aatgcaatac agcgcagaaa atctgtatct
     4441 aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga aggtaactct ataaattaag
     4501 taaaggagtg aaacagtttc ataagtaaaa tatccagtgt gctccatctc attcttaata
     4561 gatttattaa gatcatcttt ttagatggca ctttcatcag gaatgaagaa gaaacccttg
     4621 cttaaatgaa tctgatgaac ataagggaaa ccagtattca cgctggatca gcgtcgtttt
     4681 aggtgagttg ttaataaaga tttggaattg tgacacagtg caaattcaga cacataaaaa
     4741 aacgtcatcg cttgcattag aaaggtttct ggccgacctt ataaccatta attacgaagc
     4801 gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg attaactatg ctgattccgt
     4861 caaaactcag tcgtccggtt cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta
     4921 aactttccgg cgcgaacaac ttccggctgg cgctgatcac gagtcctgcg ggctacggaa
     4981 agaccaccct catttcccag tgggcggcag gcaaaaacga tatcggctgg tactcgctgg
     5041 atgaaggtga taaccagcaa gagcgtttcg ccagctatct cattgccgcc gtgcagcagg
     5101 caaccaacgg tcactgtgcg atatgtgaga cgatggcgca aaaacggcaa tatgccagcc
     5161 tgacgtcact cttcgcccag cttttcattg agctggcgga atggcatagc ccactttatc
     5221 tggtcatcga tgactatcat ctgatcacta atccagtgat ccacgagtca atgcgcttct
     5281 ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt gtcacgcaac cttccgcaac
     5341 tgggcattgc caatctgcgt gttcgtgatc aactgctgga aattggcagt cagcaactgg
     5401 catttaccca tcaggaagcg aagcagtttt ttgattgccg tctgtcatcg ccgattgaag
     5461 ccgcagaaag cagtcggatt tgcgatgacg tttccggttg ggcgacggca ctacagctaa
     5521 tcgccctctc cgcccggcag aatacccact cagcccataa gtcggcacgc cgcctggcgg
     5581 gaatcaatgc cagccatctt tcggattatc tggtcgatga ggttttggat aacgtcgatc
     5641 tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg ctcaatgaac gatgcactca
     5701 tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgcct cgaagagatt gagcgtcagg
     5761 ggctgttttt acaacggatg gatgataccg gcgagtggtt ctgctatcac ccgctgtttg
     5821 gtaacttcct gcgccagcgc tgccagtggg aactggcggc ggagctgccg gaaatccacc
     5881 gtgccgccgc agaaagctgg atggcccagg gatttcccag cgaagcgatt catcatgcgc
     5941 tggcggcagg cgatgcgctg atgctgcgcg atattctgct taatcacgcc tggagtctgt
     6001 tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa ggccctgccg tgggacagct
     6061 tgctggaaaa tccgcagttg gtgttattgc aggcgtggct gatgcaaagc caacatcgct
     6121 acggcgaagt taacaccctg ctagcccgtg ctgaacatga aatcaaggac atcagagaag
     6181 acaccatgca cgcagaattt aacgctctgc gcgcccaggt ggcgattaac gatggtaatc
     6241 cggatgaagc ggaacggctg gcaaaactgg cactggaaga gctgccgccg ggctggttct
     6301 atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct gcactgcaaa ggcgaattga
     6361 cccgctcact ggcgctaatg cagcaaaccg aacagatggc acgccagcac gatgtctggc
     6421 actacgcttt gtggagttta atccagcaaa gtgaaattct gtttgcccaa gggttcctgc
     6481 aaaccgcgtg ggaaacgcag gaaaaagcat tccagctgat caacgagcag catctggaac
     6541 agctgccaat gcatgagttt ctggtgcgca tccgagcaca actgttatgg gcctgggcgc
     6601 ggctggatga agccgaagcg tcggcgcgta gcgggattga agtcttgtcg tcttatcagc
     6661 cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg ctcgctggcc cgtggtgatt
     6721 tagataacgc ccgtagccag ctgaaccgtc tggaaaatct gctggggaat ggcaaatatc
     6781 acagcgactg gatctctaac gccaacaaag tccgggtgat ttactggcaa atgaccggtg
     6841 ataaagccgc cgctgccaac tggttgcgtc atacggctaa accggagttt gcgaacaacc
     6901 acttcctgca aggtcaatgg cgcaacattg cccgtgcaca aatcttgctg ggcgagtttg
     6961 aacctgcgga aattgttctc gaagaactca atgaaaatgc ccggagtctg cggttgatga
     7021 gcgatctcaa ccgtaacctg ttgctgctta atcaactgta ctggcaggcc ggacgtaaaa
     7081 gtgacgccca gcgcgtgttg ctggacgcat taaaactggc gaatcgcacc ggatttatca
     7141 gccattttgt catcgaaggc gaagcgatgg cgcaacaact gcgtcagctg attcagctta
     7201 atacgctgcc ggaactggaa cagcatcgcg cgcagcgtat tctgcgagaa atcaatcaac
     7261 atcatcggca taaattcgcc catttcgatg agaatttcgt tgaacgtctg ctaaatcatc
     7321 ctgaagtacc tgaactgatc cgcaccagcc cgctgacgca acgtgaatgg caggtactgg
     7381 ggctgatcta ctctggttac agcaatgagc aaattgccgg agaactggaa gtcgcggcaa
     7441 ccaccatcaa aacgcatatc cgcaatctgt atcagaaact cggcgtggcc catcgccagg
     7501 atgcggtaca acacgcccag caattgctga agatgatggg gtacggcgtg taagtttagc
     7561 cggataacgc gccagatccg gcttacatct ctgcatcatt caatgctcac ccgcgttacg
     7621 ccatctgttt ctatcaaact aaaccgcacc ggcaagaaac gctccaccac cgcgatattg
     7681 gtcagcagat ggcatgaggg atgggcgacc gtaaattccc ccgcgcccgc cagcgccatc
     7741 ggtagcacca gttggtcagc aagatattcc cccaccgccg ccgtgcttgc caggtagcgt
     7801 ttcacctctt tcaccaactg tgccgcaacc acctcggcac tgacgcgctt ttcaccgacg
     7861 acaaaaaagc gttcggtgat attttcactt tcgacttcaa gcgagacggt attacccggc
     7921 ccctggtcgc gcggcaggtt atgaatattc tgttcatgca gggaaaaact ccccgccagt
     7981 gtagcgattt cacgctcagc aacatggcgc ggcacaccag ctaatagaac ttctccacgc
     8041 atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt taaacgatgc cacgggcgag
     8101 acttccgttg ccaccacacc gcctccggca gggtaaaaac cgtggcgtaa cagcgtggtt
     8161 tgctgatgaa ttcccatttt cgccagcagc ggctccagca cccggcggat aaaatccgca
     8221 ggtggcgccg acgggttatc ggtaccgccg ctcacttcaa cacgcgaagg tccatcggca
     8281 aaccacagcg cgggcagcac agtttgcagc accagcgtac aacttccggc gctaccgata
     8341 gcaaagcggt aatcgccgcc ccgcacggtg ccaggccgga agagcagacg ctgcgatccc
     8401 agctccgcac cttccaccgt tgccctacaa atttccgcag ccgcttttac cgcggtcaga
     8461 tgctggcgca gcagccccgg tttcgcccgt ccggctcgaa tgccagtgat ggtaaatggc
     8521 tggccggtta tcatcgacag gctcagcgcc gagcgcagga tctgtccgcc gccttcgccc
     8581 tgtgcgccat ccagcgcaat catccttttc atcatttatc cttttacgca caccacctga
     8641 cgcagggtgt agataacttc caccagatcg ctttgtgccg ccatcaccgc atcaatatct
     8701 ttatacgcca tcgggatttc gtcgatcact tcggcatctt tacggcattc cacatgcgcg
     8761 gtggcacgaa tttgatcttc cacgctgaac agttttttcg ctttagtacg gctcattacc
     8821 cgcccggcac cgtggctgca cgaacagaac gactcttcat ttcccaaccc acggacgata
     8881 aagcttttcg ctcccatcga accgggaata attccatatt gaccagcacg cgcagacacc
     8941 gcgcctttac gcgtcacgta gatctcttca ccaaagtgct gttctttttg cacatagttg
     9001 tggtgacagt tgatctcttc catcgccagc gtttgtggct gtctgaccgt tttctgcgta
     9061 atgctctgca atgccgttac cacgttttcc atcatcgcat cgcggttaag gctggcaaaa
     9121 agctgcgccc aggccacggc tttcaggtaa tcatcaaagt attccgtacc ttccataaag
     9181 tacgccagat cacgcgacgg caacgtctca agcgtttcct gcatctcttt ttgtgccaga
     9241 tcgataaagt aagtcccgat ggcgttacca attccgcgtg aaccggagtg cagcataatc
     9301 cacacctgct ccgactcatc aaggcagatt tcaataaagt ggttaccggt tcccagcgtt
     9361 cccaggtgtt tatagttatt ggtattcagg aaacggggat atttttgcgt taaccactga
     9421 taaccggctt caagctcagc ccatttcgca tcgacgttaa caggcgggtt ttcccatgcg
     9481 cctttatcac gtttacaacg gccagtggta cgcccgtgcg gcacggccgt ttcaatcgcc
     9541 tgacgcagct ctgccaggtt ttcaggcagg tctgccgccg ttaacgcggt acgcagcgcg
     9601 ttcattccac agccaatatc cacgcccacc gccgccggaa taatcgcccc tttggtcggg
     9661 atcacgctac caatggtgga acctttaccc aggtgtacat caggcattac cgcaatgtgt
     9721 ttgaaaataa acggcatctt cgccgtatta ataagttgct gacgcgcatc ggcttctacc
     9781 ggcacgcctt tggtccacat ttttaccggg gcattttcag tggtcagtaa ttcgtaattc
     9841 attttgtttt tctcttttcg ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa
     9901 attaaataag cagattaatt ttttaattca ttgtttttaa ataggttaaa tctttacgtc
     9961 cgtaaacgga gattttccac aaaaccaatt caccgataat gaaatatcgt cttttataag
    10021 gatatctaag atgcgtaaaa cagtggcttt tggctttgtc ggtaccgtgc tggattatgc
    10081 cgggcgcggc agtcagcgct ggtcgaaatg gcttccgaca ctctgtttat gccagcaaga
    10141 atcgttggtc atcgatcgac tggaattatt gcatgacgcc cgctcgcgct cgctatttga
    10201 aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca gaagtggtga gcgttgagat
    10261 tgaactgcat aacccgtggg atttcgaaga ggtttacgct tgcctgcatg atttcgcccg
    10321 tggttacgag tttcagccag aaaaagaaga ctatttaatt cacattacca ccggcaccca
    10381 cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt tacctgcccg cccggctgat
    10441 acaatcttca cctccgcgca aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat
    10501 cgatctcgat ttaagccgtt ataacgccat cgccagccgc tttgccgagg aacgccagca
    10561 aacgcttgat tttcttaagt ccggcattgc cacgcgtaac ccccacttca accgcatgat
    10621 tgagcagatc gaaaaagtgg cgatcaaatc ccgcgcgccg attctgctta acggtccaac
    10681 cggcgcgggc aagtcatttc tggcgcgacg catcttcgag ttaaaacagg cgcggcatca
    10741 gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg cgcggcgata ccgccatgtc
    10801 gacgctgttt ggtcatgtaa aaggcgcgtt taccggggcg cgggaatctc gtgaaggttt
    10861 attacgcagc gccaacggcg gaatgttgtt tcttgatgag attggcgaac tgggcgcaga
    10921 cgaacaagca atgctgctaa aagccattga agagaaaacc ttttacccgt ttggcagcga
    10981 tcgccaggtg agcagtgatt ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct
    11041 ggttgccgaa ggcaaattcc gcgaagatct gtacgcgcgg atcaatctct ggaccttcac
    11101 cctgccgggg ctacgccagc gccaggaaga tattgaacct aacctggatt atgaagtgga
    11161 gcgccacgcc tcactcaccg gcgacagcgt gcgttttaac accgaagcgc ggcgtgcctg
    11221 gctggccttt gcaacatctc ctcaggcgac atggcgcggt aactttcgcg agctttctgc
    11281 cagcgtcacg cggatggcaa cctttgccac tagcggacgc atcactctgg acgtggttga
    11341 agatgagata aaccgtctgc gctataactg gcaggagagc cgtccctccg cgcttacgca
    11401 actgctgggc gcagaggcag aaaacatcga tctcttcgac cgtatgcaac tggaacacgt
    11461 tatcgctatc tgccgccagg caaagtcgct ttctgctgcc ggacgacagc tttttgatgt
    11521 ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg
    11581 ttttggtctg acctgggaag cattgcagga tcagcacagc tccagttgaa tatggtggtc
    11641 cgtcagcacc tgcatcacgc ttactggcgg ctgggcgtcg gtgtagacgg catctaccat
    11701 gctgatgctg cccatattga ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag
    11761 cataacgtgg cgcgagttct caataatggc gcgtttggtg cgaacttcgt ggtaatcgaa
    11821 ctccagcagc gagccgtcgc tatcgatgcc gcttatcccc agaatgccga aatcaaggcg
    11881 gaactgggag ataaaatcga gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa
    11941 ttcgccaccg gcgagaatga tgcgaaaatc ttcttttacc atcaacgtgt tagcaacgtt
    12001 gagattgttg gtgacaatgc gcaaattgct gtgattgagc agtgcgtgcg ctaccgcttc
    12061 cggcgtggtg ccgatatcga taaacagcgt cgagccattg gggatttgct ccgccacttt
    12121 gcgggcgatg cgctcttttt cttcggtctg ggtggccttg cgatcgtgcc acggcgtgtt
    12181 aaccgaactg gaaggcagcg ccgcaccgcc atgatggcgc aggatcaggt tttgctccgc
    12241 cagctcattg aggtcgcggc gaatagtctg cgggctgacg gagaaatgct ctaccagctc
    12301 ttcggtactg acataaccct gctgtttaac cagttcgata ataccgttgt gacgttgtgt
    12361 ttgtttcatt tataaatccc tggaattatt ttcgttttcg cgcattgagc gaatcaacaa
    12421 aagccatcgc taaacccacg gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc
    12481 caaacaaatc aaaccatccg gcgacaatcc agatcagcgc aaagataatt aacccacgtt
    12541 gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag ccagacgtag cccatcagcg
    12601 catacaccac gccagaaagc ccgccaaacc acggcccgct gaatttttgc tgcacatagc
    12661 cgcttaacag ggcgctgata agcgtaatga caattagctt accgctaccg aggcgttttt
    12721 ccaccgcacc gccgagatac caccaccaga gcaggttaaa gaggatatgc atcagcgaga
    12781 agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa tttcagtgtt ggatcgaatg
    12841 gccaggccag ccataacatc acttcctgat cgccgagaat ttgcatggca ataaacacca
    12901 ccacgcaggc gatcatcatc acccaggtta ccggacctgc gcgttcacgc aaggcggcaa
    12961 agaaaggata acggcgataa tgcaggccac tgccggtatg gcctgcctgc cagctcgccg
    13021 ccagataacg cggatctgcc gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct
    13081 cggcctggga ctcatccgcc agccagacat cgctttggtt atgttgttga atcgtgagga
    13141 taacaccctg cgtcgccatg taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag
    13201 aggtaatcat caacatcgtt gctgtcgctt attccacaca aaaggggaca gtataaagcg
    13261 ttacgcgccg tacgccacct ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc
    13321 aatgctatag accacatcgt agccctgttg cagcagatac tgcgccgcgc ctttgctgct
    13381 attgccgtga taacacatca ccatcaccgg agtgtcaaag tcgttatcac gcataaaagc
    13441 gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca tgtcccattg cgaaactctg
    13501 tggatcgcga atatcgacca gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc
    13561 aacgttaata cattcgaact gatccatgcg tctctctttc tttacaaaca agtgggcaaa
    13621 tttaccgcac agtttacgtc gaagcggcag ataaacgcca taatgttata catatcactc
    13681 taaaatgttt tttcaatgtt acctaaagcg cgattctttg ctaatatgtt cgataacgaa
    13741 catttatgag ctttaacgaa agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg
    13801 atagggggcg gcatcaatgg tgctggtatc gcggcagacg ccgctggacg cggtttatcc
    13861 gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct cttccgccag ttcaaaactc
    13921 attcacggtg gcctgcgcta ccttgagcac tatgaattcc gcctggtcag cgaggcgctg
    13981 gctgaacgtg aagtgctgct gaaaatggcc ccgcatatcg ccttcccgat gcgttttcgc
    14041 ctgccacatc gtccgcatct gcgcccggcg tggatgattc gcattggtct gtttatgtac
    14101 gatcatctgg gtaaacgcac cagcttgccg ggatcaactg gtttgcgttt tggcgcaaat
    14161 tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt ctgactgttg ggtagacgac
    14221 gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta aaggcggcga agtgcttact
    14281 cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt ggattgtgga agcggaagat
    14341 atcgataccg gcaaaaaata tagctggcaa gcgcgcggct tggttaacgc caccggcccg
    14401 tgggtgaaac agttcttcga cgacgggatg catctgcctt cgccttatgg cattcgcctg
    14461 atcaaaggca gccatattgt ggtgccgcgc gtgcataccc agaagcaagc ctacattctg
    14521 caaaacgaag ataaacgtat tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc
    14581 ggcactaccg atgtcgagta caaaggcgat ccgaaagcgg tgaagattga agagagtgaa
    14641 atcaattacc tgctgaatgt gtataacacg cactttaaaa agcagttaag ccgtgacgat
    14701 atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag
    14761 gctattaccc gtgattacac ccttgatatt catgatgaaa atggcaaagc accgctgctg
    14821 tcggtattcg gcggtaagct gaccacctac cgaaaactgg cggaacatgc gctggaaaaa
    14881 ctaacgccgt attatcaggg tattggcccg gcatggacga aagagagtgt gctaccgggt
    14941 ggcgccattg aaggcgaccg cgacgattat gccgctcgcc tgcgccgccg ctatccgttc
    15001 ctgactgaat cgctggcgcg tcattacgct cgcacttacg gcagcaacag cgagctgctg
    15061 ctcggcaatg cgggaacggt aagcgatctc ggggaagatt tcggtcatga gttctacgaa
    15121 gcggagctga aatacctggt ggatcacgaa tgggtccgcc gcgccgacga cgccctgtgg
    15181 cgtcgcacaa aacaaggcat gtggctaaat gcggatcaac aatctcgtgt gagtcagtgg
    15241 ctggtggagt atacgcagca gaagctgtcg ctggcgtcgt gatttaatgt aaggtggcca
    15301 ggtcagattt caatctgacc tgagactgat gacaaaccaa aaaactgcca gatgcgctac
    15361 gcttatctgg cctacgttgt ttatacaata tattgaattt gcatgatttt gtaggcctga
    15421 taaggcgttc gcgccgcatc aggcatgaac aaagcgccac gttgtcaaca atctgaccta
    15481 ctttttgatt cagggaatta ccgcaaagcc cacgccatca tacccaacgt aacaaaactt
    15541 aagcacgttc cccactcact caacttccgt atctggcatc tatttttgct gcaccactgg
    15601 caataatatc ccaacgcaaa accaagctgt gcgcttattg gaccgataaa atccgtccac
    15661 ggctgaggcc aaaatggcga aaaattcagg ggtagccaga caacaagcag aaagaaattg
    15721 cggatacttt ccagcaacgc aattttcgct ggcgcgatgt aaaaaatgat attccagagg
    15781 atcaaccata aatagaccgg tgcgaatacc aaccaacaat ggagcctgct tttcaattca
    15841 gattgagttc tacgagtaaa ttgccaacca acgcaacaca ttacacagaa gaaaagcgca
    15901 tattcagccg ataaccaaag ttgtaattgc agaagtgaaa tacgcctgat catgagcggg
    15961 attgagaaca gcatttcccc cagaaaactc agcgtgataa tacaagcagc cagcgcaacc
    16021 accatcggcc acaccaacaa cggcacaata acaaagccga gaaataacgg ataaatataa
    16081 agtctgatta tattcatcat tattgtcctg atgattcggg ggatgcttca tacagcggca
    16141 cataccccac atcctctacc aactgttgcc cctgctgact tataaaccag tcaacaaatt
    16201 tctgcgtttc cggcgtggga ttttcaagcg tcaccatata ggcatccaca atgtatggat
    16261 aagtgccgtt gcggatattt tcctcggtag gcgcccattt atagccaaaa gtttaatctg
    16321 cttgcgcatt ttgagcatat tgtcattcat acgagtgacg taatagtgga aggtgtaccc
    16381 aatggaagat ggcgtgtttt gatagtcggc aacttcagta attaaaccgc ccataagatc
    16441 aatcacagtg gattttttag ctggcagcat tggggtgtct tgcataacca accccttcat
    16501 aatcgtttgg cttccagaat cttcagggcg ctgccagact tttatatgtt cgtcactacc
    16561 acctacttta ttccagcggc tagttttgcc gctaaaaata tctttaacct ggtgctcaga
    16621 gagagaattt accgggttat taatatcaac gataaagaca aaggcttcgc gggcgaaagc
    16681 ggtatatttc agtttaacac ccgcttcttc tgcacgtttt ttctgtcctt ccgacggttg
    16741 tgcaacaaaa ataaccgtcg cggaaccttt aatcagacta tcgtaggctt cagctgttcg
    16801 ggtttgctgc aaaccatacg cctgccaggc cagcgaggtt cgatccttat cttttggctc
    16861 gggtactaat ttataatacg cggaggcata cagcgggtat aaggctgtag cgccgtacac
    16921 tctcggccat cttgaccaat catcgctgta acgtaatgca ggttgccccc gcagagacgt
    16981 cagtccatgg ctctcttctt ccctaacgct gttatagtac ccttcccggt cgacacatcc
    17041 cgccagtaac gccgtgccta atagtaaaac accggtgaat ttattcatct cgctgattcc
    17101 tttctctcga gcgttattcc attgttggat acagcggcac atatcccaca tcttctacca
    17161 gactctgccc ctgcggcgtt aaaaaccatt cgaccagttt ttgtgtttct gacgttgtat
    17221 tttctctcgt caccataaat gcatcgacga tgtacgcata tttgccgttg cgaatgtttt
    17281 ccgccgtcgg tgtaataccg ttaatcgcta gcaattttat atttttatca gcattcattt
    17341 gcgtcgcgta atagcggaag gtatagccta ttgcgttgtt tgtattacgg tattcggcaa
    17401 cgactttaat cattccctcc atcacgcttg ccactttcgt ttcctgcggc gagatcatgc
    17461 ggacattttt catgacctgt gattgcatca ctgtctggct gccagagtct tccgggcgct
    17521 gccaggtctg gatctcctga tcgttaccgc caacagcacg ccaattggta attgcaccac
    17581 tgaaaatgtc acgcacctgt tgttcagtca gggaattaac cggattatcc gcattgacga
    17641 tgaaaacgaa tgcttcacgg gcaaaggggg tgtacagcaa agtgacacca gattcctttg
    17701 cacgtttttt ttgcccaccg gaaggttgcg ccacgaaaat aatatcagca tcgcccttaa
    17761 cgattttgtt atatgcttct ggcgtacgag agttttccag ataatcccaa acgtgaaaat
    17821 cctctggtat tacacttaat gcataaaatg cagaagcata aattgggtac gcagccgtgg
    17881 cgccatcgat tcgcggccag ttttgcctaa actgaatttg cggtttgcca cgcagcggcg
    17941 tcagtcgatt attgagttta tcgggtcgcc aggcccagat atcaatattt tcgcgtacac
    18001 cgacaccagg attttgctgc ttatataaat gggcctgata cccggcgaca ataccaagta
    18061 agagaagtag aaacaggagt aagttacggc aacgtagtcc ggacgtgccg gataaaggat
    18121 atctggtcgg ccaggcgtat cccagcgaaa aactgatatg cgccccaacg ggaataatga
    18181 ccatccacca gtactcacca cccactaatg agactaaaaa aacgcccaac cagggaagca
    18241 taaggtttgc caaccaacca gctcccatct ggcgaaattg ataatctgcc agatgtagcg
    18301 tgattatcca gccaaccagg gttaatagcg cgggtaagag aaaaaagcat tgccgccaga
    18361 cggctatttc tctgttttct ggcgcgctat caccggccca cacgcgacca gagaaaaata
    18421 ttattatcgc ccccagcgtc agcataaaga cgttgagcag aaggtaaatt gttggcgtga
    18481 taaagatatt acaaacttca ataataccgg caagtccgac acccagcatg gcaataaccg
    18541 ccgccaaaaa ctgcgccaga atggggatgc cgaaaaaagt cattaccagc gaggtcaaaa
    18601 tccattttct gttttgcatt attctttcca ttctttctga atggcgaaat tatactcccc
    18661 gagtcccctt gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt
    18721 tggccccgtt ctatttatta gtgaaattac aatctcaccg gatcaatatg ccagatatga
    18781 tcggcgtact ctttgatagt acggtcagaa gagaagtagc ccatattggc aatgttcagc
    18841 atcgcttttg cggtccactc ttcctgaagc tcgtagagtt catcgacttt atcctgacaa
    18901 tcgacatagc tgcgataatc cgccagtacc tggtagtgat cgccgaagtt gatcagcgaa
    18961 tcaaccagat cgcgatagcg acccggatct tccggactga atacaccgct gccgatttgc
    19021 gtcagcacct gatgcagctc ctcatctttc tcgtagtatt cacgcggttt gtagccctga
    19081 cgacgcagtt cttccacttc ttccgctgtg ttaccaaaaa taaagatatt gtcagcaccg
    19141 acatgatcca gcatctcgac attcgcaccg tccaacgtac cgatagtcag cgcaccgtta
    19201 agcgcaaact tcatgttact ggtgccggaa gcttccgtcc ccgccagcga aatctgttca
    19261 gacagatcgg ccgccggaat gatcaactgc gccaggctaa cgctgtagtt cgggatgaac
    19321 acgactttca gcttgtcgcc aatctgcgga tcgttgttga tcactttcgc tacgtcattg
    19381 atcaaatgaa taatgtgctt cgccatgtaa taggccgaag ccgccttacc gccaaaaata
    19441 ttcacgcgcg gtacccactt cgcatccggg tcggccttga tgcggttata gcgggtaatc
    19501 acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa tacgtttgat ttgtacatcg
    19561 aacaacgcct ttggattcac caccacattc agctgctggg cgatatactc tgccagacgc
    19621 tttttgttct ccagcttcgc ctgatgcaca gcgtgattaa ccattgggaa atcacagtgt
    19681 tgttgcagct cattaagcag gctaaggtcg gtgcgccagt tacggcccag gtgttcgtcc
    19741 agcacggctg aaagcgatgg gttcgctacc gctagccagc gacgcggcgt cacaccgttg
    19801 gtgacgttgg tgaaacgacc cgggaagatt ttcgcaaagt cggcaaacaa cgattgcacc
    19861 atcagattag agtgcagttc cgatacaccg ttaactttgt ggctcacaac aaccgccagc
    19921 caggccatac gcacacgacg accgttggat tcatcaatga tcgacgcccg tcccagcaga
    19981 tcggtatcgt tcggatactg ttcctgcaag gttttcagga aatagtcgtt gatttcaaag
    20041 atgatctgca ggtgacgcgg cagaatttta cccagcatat caaccggcca ggtttccagc
    20101 gcctcgctca tcagcgtgtg gttagtgtag gagaagacct gacaacacac ctcaaacgcg
    20161 tcgtcccagc taaattggtg ctcatcgatc agcagacgca tcatctcagg aatcgacagt
    20221 accggatggg tatcattgag atgaatcgcg attttatccg ccaggttatc gtaggtttta
    20281 tgcaactgat aatggcggct taaaatgtcc tgaatggtcg aggaaaccag gaagtattcc
    20341 tgacgcaggc gcagctcacg cccggagtag gtggagtcat ccggatacag tacgcgagat
    20401 acgttctcgg agtggttttt atcttccact gccgcgaagt agtcaccctg gttgaattta
    20461 ccgaggttaa tttcgctact ggcttgcgca ctccacaaac gcaacgtgtt ggtcgcgtcg
    20521 gtatcgtaac cagggattat ctgatcgtaa gcgactccca gaatctcttc ggtttcaatc
    20581 cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa aacggacttt atagcgcgtg
    20641 ttgtggcgtt tgaattccca cgggttaccg tattccagcc agtagtctgg cgactctttc
    20701 tggctaccgt taacgatgtt ctgcttgaac ataccgtagt catagcggat gccgtaaccg
    20761 cgccccggca accctaacgt cgccagagaa tcaaggaagc aagccgccag acgtcccagg
    20821 ccaccgttac cgaggcccgg atcattttct tcatcaatca gctcttcgag atttaacccc
    20881 atcgcttcca gtgcgccctg tacatcttcg taaattccta gcgacaacat ggcgttggag
    20941 agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa cctgacgagt ttcttgcgac
    21001 aactgggcac ggtttgaacg taaccagcgc tccacgagac gatcgcgcac agcaaataac
    21061 gttgcgttca gccattcatg tttattggcg acgaccgggt cctttccaat cgtaaacatc
    21121 agcttgtaag cgatagagtg cttaagagct tctacgctaa gcgtgggcga tgaatatgta
    21181 aacggagcat tcatataggc gtttcctgaa aactatttca agcgatagta aagctcacgg
    21241 tacgacttcg ccgcgacctg ccagctaaaa tccattgcca tagcctgacg ttgcacaaac
    21301 cgccacagtg aaggacggga ccacagtaca aaagcacgcc gaatagcccg taacagcgac
    21361 caggcattac tatcttcaaa gacaaaccca ctggcgacgc catctgcaag gttctcaaga
    21421 gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc gcactaacgg cagcgtaccg
    21481 tacttcaatc cataaagttg cgttaagccg cacggttcaa aacggctggg caccagaatg
    21541 acgtccgcgc cgcccataat gcgatgcgaa aatgcttcgt gatagccaat ctgaacgccc
    21601 acctgaccgg ggtattccgc tgccgccgca aggaaacctt cctgcagcac cggatcgccc
    21661 gcgccgagta gcgccagctg cccgccctgc tccagaaggc ccggtaaggc ttccagcacc
    21721 aggtcgagac ctttctggct ggtcagacgg ctcaccactg caaaaagcgg cactttatcg
    21781 tcaaccttaa gccccattgc gatttgtaac tggcgcttat tttccgcttt atcttccaac
    21841 gtatcgcggg tgtaacgcga ggccaacagt aagtccgtct caggactcca gattttctcg
    21901 tccacgccgt tcagtacgcc ggaaagacgc ccttcacggt gacgctgttg caacagacct
    21961 tccataccgt aggcaaactg cggttcggtg atctcgcgag cgtaggttgg actgaccgcc
    22021 gtaatgtgat cggcatagta cagaccggcc ttcaggaaag agatttgtcc gttgaattcc
    22081 agcccatgaa tattaaagaa tgaccatggc aattggatgt cattcatgtg atgtgcataa
    22141 aacatgcctt gataggccaa gttgtgcaca gtaaacaccg acttcgccgg acgcccgcgc
    22201 gccgccagat acgcaggcgc aaggcctgca tgccagtcgt gcgcatgcac cacatcagga
    22261 cgccagaatg ggtcaagccc gctggccatt tctgccccaa cccaccccag cagcgcaaaa
    22321 cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt gatacgggct tcccggacga
    22381 tcatagagat gcggcgcgtc aatcaggtat atgccaaccc cgttgtaatg accgaacaac
    22441 agcgtgatat gtccggcgaa ggtatcacga cgggatacta cttgcgcatc ggtcacgcca
    22501 cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt caacgccgtc tgcgatttgt
    22561 gctgcgggta atgccccaat aacatcagcc agaccgccgg ttttaagcag cgggaacatc
    22621 tctgaacata catgtaaaac ctgcattatc gctcctgttt atgccctaac ttccgtagca
    22681 tttcgcgcgt taccagcacg atgccttctt ctgaacgata gaaacgacgt gcatcttcct
    22741 ctgcgttttc accaatcacc atgccttccg gaataacaca agcacgatcg atgacgcagc
    22801 ggcgcagacg gcacgagcga cctacccata cttccggtaa caatacggcg gaatcaatgt
    22861 tgcagaatga attcacgcga acgcgcgaga acagaacgga ctgcaccacc accgaaccgg
    22921 agatcacaca gccgccggat accagtgagt taagggtcat cccgtggcta ccggagcgat
    22981 cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt gcgaattggc caattgcgat
    23041 cgtacatatc cagttccggc accacagagg ccagatcgag gttcgctttc cagtaagctt
    23101 ccagcgtacc cacatcgcgc cagtacggct cggcatccgg gtcggattgt acgcaagaga
    23161 gcgggaacgg gtgcgcatag gccagaccgg cttcggtgat cttgggaatc aaatctttgc
    23221 caaagtcgtg gctggagttc tcatcacgat cgtcttcttc cagcagttca tacagatagt
    23281 cggcgtcaaa gacgtagata cccatactcg ccagagattt gctcggatcg ttcggcattg
    23341 acggtgggtt agcaggtttt tcaacgaatt cgataatttt atcgttctca tcaaccgcca
    23401 taacgccaaa tgcggaggct tcttcaatcg gtactggcat acaagcaacg gtgcaacgtg
    23461 cgcctttttc gacgtgatcg ataagcatac gcgagtaatc ttgcttgtag atatggtcgc
    23521 ccgccaggat caccacgtat tccgctttat aacggcggat aatatcgagg ttttgggtga
    23581 ccgcatctgc ggtgccgcga taccagtttt cccctttcat tctctgctgc gctggcagca
    23641 gatcgacaaa ctcgttcatt tcttcattaa agaatgacca gccgcgctga atgtgctgca
    23701 ccagagtgtg ggactggtac tgggtgatca cgcccatacg acggatcccg gagttgatgc
    23761 agttagacag cgcaaagtcg ataatgcgga acttaccgcc gaagtgtacg gccggttttg
    23821 ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc tcccgccagt atcagggcaa
    23881 cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc gttcttctct aaactaacca
    23941 tgactaactc cttttttatc atctctggaa cacacacaat ccgtgtgcag gtccctgcca
    24001 gacagccgta atcactgggt tatcctctcc agcgaatggg ggaatggcgt gccactcccc
    24061 agcaggtaaa acaatctctg ttacctcaag cgtggcgtta attgcgatca aaaagcgatc
    24121 cgagagcaga atttgcagct gtttcggccc gttttgccac tcatccgtgc ttaaaggttg
    24181 agcatatcga tttagccaac ggacattgcc gtcgccttct tcccaccagc gattctccac
    24241 caaagcggga atacgcttgc gcagatggat taacgcggcg gtaaatgcgg ttaaaccact
    24301 gcttgcctgc gaccagtcca accaggttaa ttggttatcc tgacagtagg cattgttatt
    24361 gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac atcggcgtgc cctgggagag
    24421 caacaacgtt gttaacaggg catgaatgct gtcgcgccgc cgttcaacca gatcaagagt
    24481 accacctaac ccttctttac catgattgtt actgtaattg ttgttggtcc cgtcgcgatt
    24541 ttcttctccg tttgcttcat tgtgtttatg gttgaagcaa acgcagtcgc gaagcgtaaa
    24601 accgtcatgt gcggtgacga gattaatcgc ggcactcggc agacgaccat tacgtttaaa
    24661 aacatcgctg gaggcagcaa aacgcccggc aaacgccccc agaggcaaat cataatgcag
    24721 ccagaaacga cgggcagcat cgcggaaatg atcgttccac tcggcaaaca gcggcgggaa
    24781 atttcccacc tgataaccac caggagcgat atcccacggt tcagcaatta acttcacctg
    24841 cgagagcacc gggcagttct ggatagcggt aaacaacggc gcatcctgac ggaactctgg
    24901 cgtacggccc atgactgccg ccagatcaaa gcggaaacca tcgacgtggc aggtttctac
    24961 ccaataacgc aggcaggcgc tggcataatc caccaccgcc ggatgactca aattgagcgt
    25021 gttgccgcaa ccggtccagt tgtgataatc gccgtcttct cttatccaat aatagctacg
    25081 gttatcgatc ccacgcagcg agaataacgg gccgtcgagg tccagttccg cactatggtt
    25141 gagcacgata tcaagaatga cttcgatacc cgctttatgc agtgctttga ttgcatcgcg
    25201 aaactcatcc agcgccgttt ctggcgagca ggcatacgcc ggatgcagcg caaacatcgc
    25261 caccgggttg taaccccagt agttacttag ccccatgcgt tgcagacgtg gttcactggc
    25321 aaactgcgcc actggcagca gttccagcgc ggtaatgccc aattgtttca aatagttgat
    25381 catcaccgga tgcccgaggg ctttataagt gccacggatc tcgaccggga tctccgggtg
    25441 caagtacgtt aatcctttga catgggcttc ataaatgatg gtgctgcccc acggcgtgcg
    25501 cggcggggca tcatcttccc agtcatagtg atcaaccacc actacgcatt tcggcgcaat
    25561 ggcggcgttg tcgcgatagt caggttcatt atgaccggcg tgcagcagcg ggttatcttt
    25621 aaactccccg tcaatttgcc gcgcgcaagg atcaatcaac aacttcgccg ggttaaagcg
    25681 atgcccctcg gcgggttgcc aggggccatg aacgcgataa ccataacgca aacccgggcg
    25741 cgcatccggc agataaccgt gccaaatgtc gccactgtgc cctggcaagt catagcgatg
    25801 ttcctggcca ttggcgtcaa agacacacag ttctacccgc tcggcatgaa cggagaaaag
    25861 tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg ggagcgggtt tgccaatggc
    25921 gagttgtgtc attctgcctc ccgaaccagc cagatagtgg ccagcggtgg tagcgtcagg
    25981 cttagtgaat gctgacgacc gtggctggca atctcatcgc tgtgtaccgt gccgccattg
    26041 cctgcattac tgccgtgata gtgcatggaa tcggtattga ggatttcacg ccatttgccc
    26101 ggctgattaa tgccgaagcg ataatcatga cgcggtaccg gcgtaaagtt actggcaacg
    26161 atgatttcgt taccctcttt atcgcgacgc acaaagatca gcaccgagcg ttctttgtca
    26221 tccaccacca gccattcaaa gccgtacggg tcaaaatcca gttcatgcat tgctttatgg
    26281 tggcggtagg tgaggttcag atcgcgcacc agacgctgga caccgtggtg ccagttatcg
    26341 ccgccttcca acagatgcca gtcgaggctg gcgtcatggt tccactcgcg gccctgggca
    26401 aattcgttac ccatgaacag tagtttcttg cccgggaatg cccacatcca gccatagtag
    26461 gcgcgcaggt tcgcgaattt ctgccatgcg tcgcccggca tgcggtcgag aatcgatttt
    26521 ttaccgtgga ccacttcatc atgcgacaac ggcaggacga agttttcagt gtagttgtag
    26581 agaatcccga aggtcagttt atcgtgatga tactgacgat aaaccgggtc gagcttcatg
    26641 tagtccaggg tgtcatgcat ccagccgagg ttccacttgt accagaagcc cagaccgccc
    26701 atatcctgcg gacgagaaac gccagggaaa tcggtagact cctcagccat tgtcaccgca
    26761 ccggaaacct gctcaccaag aatacggttg gtattacgca agaattcaat cgcttcaaga
    26821 ttctcgcgcc cgccaaattc gttcggaatc cactccccct ctttacggct gtagtcgcga
    26881 taaatcattg acgccaccgc atcgacgcgc agcgcatcaa taccaaaacg ttcaatccag
    26941 taaagcgcgt taccgacgag gaagttactg acttcacggc gaccatagtt gtagatcagc
    27001 gtgttccagt cctgatgata gccttcacgc ggatcgctgt gttcatacaa gttcgtgcca
    27061 tcaaattcgg caagcgcaaa gtcatcggtc gggaagtggc ctggcaccca gtcgagaatc
    27121 acgttcagac cagctgcgtg tgcggcatca atgaaataac ggaagtcgtc gcgagtacca
    27181 aaacggcggg ttggtgcata caggccggtt ggctgataac cccaactgcc atcgaaggga
    27241 tgctcgttaa tgggcagtag ttcgaggtgg gtaaagccca tccatttagc ataaggcacc
    27301 agttgatcgg ccagctcgcg gtagctcaac cagaaattgt tgtcggtgtg gcgacgccag
    27361 gaacccaggt gaacttcata aatagagatt ggcgcatcaa actgattcgc ttttttgcgc
    27421 tcttcagtct gtacaacctt ttccggcagc ccgcaaataa gagacgcggt ttccgggcgc
    27481 atttgcgctt cgaaggcata agggtcggac ttcagacgca agttgccatt ggcatcaatc
    27541 atctcgtatt tatagagctg accgttatgc gccccaggga taaacagttc ccagatgccg
    27601 ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc agtagttgaa ttgcccaacc
    27661 accgagaccc gacgggcgtt tggagcccag acagagaaac gcgtacctgt gacgccatcc
    27721 atagtatctg catgcgcgcc taaggtttca tacgggcgca ggtgagtacc ttcagataat
    27781 agccaggcat ccatttcctg gattagcgga ccaaaacggt aaggatcatc aatcaggttt
    27841 tgctgaccat gccagacaac agccaactga tagcggaaaa aattcttacg tcgcggaatg
    27901 acgccgctaa agaatccccg tgagtcgaga cactccagtt ttgcgagttt gcgcccggtt
    27961 ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa gggcacggac ttccagtccc
    28021 gcggtggttt tatgcattcc cagtacggaa aaaggatccg caaaatggcc tgcaattagc
    28081 gcgttaatca cgtctctatc gatacgatcg gacatgcttg tcttcctgtt ttattgtgtc
    28141 accccatcca attggatttt tgacttctgg ttgtgacatt tttttgacct gaacggcgca
    28201 gcactctgtg catcctctct gcgtcgtcct cacttcaggt aaggctgtga atactcatgt
    28261 attcagccac ccttaaagaa tagccaatgc tctatttaac tcccggtaaa tcatgaaaca
    28321 tctgcgctta ctcctgtatt acgcactaac aggggcggca tcgcgcccca gatttaatga
    28381 ataaagatta cgccagttga cgaagcatcc gacgcagcgg ctccgcggcc ccccacagca
    28441 gctggtcgcc cactgtaaag gctgacagga actctggtcc catattcagc ttacgcaggc
    28501 ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg ggttaactca cgcatagtga
    28561 tttcccgatc gttcggaacg actttcgccc acggattgtg cgcagccagc agttcttcca
    28621 cggtcggaat agacacatct tttttcaatt taatagtgaa tgcctggctg tggcagcgca
    28681 atgccccgac acgcacacat aaaccatcta ccggaattac ggaagatgtg ttgaggatct
    28741 tgttggtttc cgcctgccct ttccactctt cgcggctctg accgttatcg agctgtttgt
    28801 cgatccacgg aatcaggcta cccgccagcg gcacgccaaa gttatccacc ggcagctcac
    28861 cgctacgggt taaggttgtg actttgcgtt cgatatcgag aatagcagag gacggggtcg
    28921 cgagttcatc tgccacatgg ccatacagat ggcccatctg ggttaataac tcacgcatat
    28981 gtcgcgcacc accgccggaa gcggcctggt aggttgcaac ggacacccaa tcaacaagat
    29041 cattggcgaa taaaccaccc aacgacatca acatcaggct tacggtacag ttaccgccaa
    29101 caaaagtcct gatgccatta tttaatccgt cggtaatgac gtcctgattg acggggtcaa
    29161 gaatgatgat ggcgtcatct ttcatgcgca gagacgatgc tgcgtcaatc cagtaacctt
    29221 gccatccgct ttcacgaagc tttggataga tttcgttggt ataatcgccg ccctgacagg
    29281 tcacaatgat atcgagggcc tttagcgcct ccagatcaaa ggcatcctga agtgtgccag
    29341 tggttccgcc aaaagacggc gcagcctggc caagctgaga agtagaaaag aagacagggc
    29401 gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat gagaacggag ccgaccatac
    29461 cgcgccagcc gataaaacca acatttttca taagcgtttt ttcctgcaaa gatgtgtgct
    29521 gtataaatgt gccggtctcc tcttggcaca tctttcacca tacaaaaagc agccaaagtc
    29581 gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat gcgacttatc ctgctttgtt
    29641 agcacgcaga aagtccgcgg caattatcag ggaatttgag ttatgaatga aatcatttct
    29701 gcagcagttt tattgatcct gattatggat ccgctcggaa acctacctat tttcatgtcc
    29761 gtactgaaac atactgaacc gaaaagacgg cgggcaatca tggtgcgaga gttgcttatt
    29821 gctctcctgg tgatgctggt gttcctgttt gcgggtgaga aaattctggc atttctcagc
    29881 ctgcgagcag aaaccgtctc catttctggc ggcatcattc tgtttctgat cgccattaag
    29941 atgattttcc ccagcgcttc aggaaatagc agcgggcttc ctgcaggtga agagccattt
    30001 atcgtgccgt tggcaattcc gttagtcgcc gggccgacta ttctcgccac gctgatgttg
    30061 ttgtctcatc agtacccgaa tcagatgggg catctggtga ttgctctgct gctggcctgg
    30121 ggcggcacct ttgtcatcct gctacagtct tcgctatttt tacgtctgct gggcgagaaa
    30181 ggggtgaacg cacttgaacg cctgatggga ttgattctgg tgatgatggc aacccagatg
    30241 ttcctcgacg gcattcgaat gtggatgaag gggtaacgag gagagttttt cgaaaagaac
    30301 ccggtagcgt cacaactacc gggcgaacaa acttaactca acagctggaa cgcaatcatc
    30361 ccaacgatgg caccgacagt gccgagaatg gtttccatca tggtccaggt tttcagcgtt
    30421 tcggcttcgg tcgcgccggt aaatttaccg aacaaccaga aaccagcgtc gttaacgtgg
    30481 ctgacaacaa tcgaaccacc agcgatacaa atcgacagtg ccgccatttg cgcaccagag
    30541 tagttcagtt gttcaatgac tggcatcacc agtcccaccg ccgttaaaca ggctacggtg
    30601 gcagacccct gaatgatgcg cactgcagcg gccagcacga agcaggtgat agcaatcggc
    30661 aggcccatgc cggttaacgc ttcgcccagt gccggaccta cgccagagtc aaccagcacc
    30721 tgtttgaaca cgccgcccgc accaatcacc agcagaatga tccccgccgg ttgcagcgcg
    30781 tgaccgcaaa tctccatcac tttgtctttt ggcatgccct gacgcatcgc caggccataa
    30841 atcgccacca gacaagcaac cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat
    30901 tcgtaagcgg tagagccttc tggcacaaaa cgcgcggcaa tggttttcag ccccaccagc
    30961 accagcggca acaggatcag cgacaggctg aatccgaagg acggcatttt gccttcgccg
    31021 agatgcggtt cgctgatgtc gtcaggaata tgcaactcca cgtaacggct gatgaaatta
    31081 ccccacagcg gcccggcaat aatcattccc ggaattgccg cacacaggcc aatcaggatc
    31141 atccagccaa aatcggcgtt catctgcgat gccagcagca ttggcgctgg ccctggcacc
    31201 aggaacgccg ccgctgccgc cacgcctgcg aataatggga ttaccagctt caccaggttc
    31261 gtaccggtgt ggcgcgccat tgagaaagca acgctaatca gcagaacaat cgccacttca
    31321 aagaacagcg gtagcgcaca gaccagcccc gcaaggccga tggcataatg cgcgcggctg
    31381 tgaccgaagg atttgagcat tttgacggca atctgatcga ctgcgccggt ttcatgtaag
    31441 atcttgccaa acatagctcc cagggcgaca accaccgcca ggaagccgag ggtgcctccc
    31501 atcccttttt ccatcgtcgc tgcgatttta tcgagcggca taccggaaaa aaggccagcc
    31561 cccatggaca ccaccattaa agccaggaaa gcgtgcatac gcgccttcat gactaaaaac
    31621 agcagcagta aaacagaccc tactgctgtt aaaacaagcg ttaatgtagt cactacttat
    31681 ttgccttttt taataacctc aatggtgctt gccacaacac cttccagcgg ttgatcgata
    31741 tccaccacca gtacatcggt ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc
    31801 accaacattt gggttttaaa gaaatggcct ttgcgcgctt tcaggcggct ttcaatcaca
    31861 tcaaaatcgc ctttcaaata gatgaaagag agattcggat taccttcacg cagcaagtcg
    31921 cgatagtgtt ttttcaatgc agaacagacg atcagcgaca ctttattagt gcgctgcata
    31981 gcaaacgcgg cgtcgttcag cgcctgcaac cacggtttgc gatcgtcgtc attcagtggt
    32041 tcgccagacg ccattttttc gatattgcgc cgtggatgga ggaaatcgcc atcaagaaac
    32101 gcggcatgaa gttgatgcgc cacttcactg gcgaccgcag atttgccgct gcccgatacg
    32161 cccatcaaga cgtaaatgtg gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa
    32221 tacaagaatt aaaaatgtta cgggtaactg ttatcggtaa cattgtccag ccggacaata
    32281 tcagaagcaa tatccatcca tgccttaagt gtataagtgt gagctacttc aaatttgtgg
    32341 gcttaaatag atccgcccgg tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca
    32401 gattcgccac gaatacgcgc cagcaggcgt tcagcgccaa tactgcccat ccgctcacgc
    32461 ggcgtcagca cgctcgcaag tcgtggctcc atcacctgac caatgtcatg accgtggaaa
    32521 ccggcaatcg ccatatcgtc aggaactttt aaccccagac gctgacattc aaacgccgcg
    32581 ccgaccgcca ggtcatcatt cgtacagaac acgccatcca gctgcggata ttcccgccgc
    32641 gcctggcgaa tcagttcaat accggaagag taagaagaag attgctcaac catcacgcta
    32701 tatggcacca ggcctgcatc cagcatcgcc tgttcgtatc ccttctgttt gatgatagta
    32761 cgttcgtcga gacgtgcgcc gagataggca atgtggcgat gcccgcgagc aataatggca
    32821 gtggtcatct ggcgtgctgc ttcaaagtta tcaaaaccga cggcgatatc aaggcatggc
    32881 gacttgctgt ccatcagttc caccacggga atacccgcca cttcaatcat ctttaaggtg
    32941 cgcggcgtgt gggtacgttc ggtgaggatc aggccgtcga tattccagga gagcatggat
    33001 tcgaggcgtt cttgctccat ttccggttta taaccgtagt gcgccagcat ggtctgataa
    33061 ccgtgcgcgt cggtgacgct ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg
    33121 agagaaggta acaggacgcc aatcgcccgg ctggtggcgt tagagaggat atcgggcgca
    33181 cgattgggaa tatagcccag ttcatcaaga gccgcggcaa tcttgccgcg tagagcgacg
    33241 gaaacctgct ccgggttgcg taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg
    33301 tcagccacat cctgaagtac gggtcttttc tttttcatcg tcctgaaggt acaaaagaga
    33361 tagattctct tagtttaaca cggacacatc agaaccttcc cggggaaaac agtgcttgtt
    33421 tacaattatt agatatacac taaatcaatt gcatcgcatt gtgctaacga cgcggcaaaa
    33481 ccccggaagc gtacatcagc acgcgaccgg agcgagcgag gaagcctgca aaaaggcagg
    33541 cttcaaagat gccgttgagt tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc
    33601 gctatccgca tggatggaga ttgcctgctc atcccagatt gccagaccat cgctggtcga
    33661 ggctttcacg ccgttaatgg tgacattgcc tttcaccacc tggatccaga cgcggcgttc
    33721 agcggcaatc tgatgcaccg actgctcatc tttcagcaac gcccagcggt acagttccat
    33781 atcctgatgc actttcaatg aaccatctcg cgcatccggc gagagcacca gctgtttgcc
    33841 ctgtacggca tcgaagcgac gctgttcata acgcggcgta ataccgtttt cttcaggcat
    33901 gatccagatc tgatacagat gcagacgctc ggtgctgctt gggttgtact ctgagtgacg
    33961 aatacccgta ccagcactca taatctggaa ctcacccgcc ggaacctgct ctttattgcc
    34021 catgctgtcc tggtgctcaa cagtaccttc cagcacgtag gtcaaaattt ccatatcttt
    34081 atgtgggtga gtgccgaagc cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag
    34141 cgcggagaag cccataaagt tcggatcgta atagttggca aaagagaaag tatgccagga
    34201 gtccagccag ccatgatttg catgaccacg ttcatttgct ttgcgtaagt agatcatttg
    34261 tattcgcccc ctgaatgatt tcgatgggct tagtgtggac ccgatctgcc tgggataata
    34321 gagggtgaaa attgacccct ctgttcaaaa aaattgaact attcgagggg ccatcaaact
    34381 tacttcgcga gagtcactgt ggagggagag gcttgctcga atccgcgttc aaggatttcc
    34441 agattcgtaa gaacttcaga ttccttgacg taatttggcg caccgtgggt gatggtttga
    34501 tacaacgcat cataaacgcg cccgtaatcg cccatctccg gcttaatctc ttctctgacc
    34561 gtcacgccct cgtcattgac atactccagc acaccgaccg aatcatccgc tgagaatccc
    34621 ggttcgcccg gcataatatt agccttcagg ctggtttcct gctggtcgat accatattta
    34681 ataaacgaac ctttcttacc gtgaacgata aatttcggat aatcgatttt caccagatgg
    34741 ctggttttga cgatggcttt tagatcgcca taaaacagtt gcgcttcgaa agtatcgtca
    34801 ggattggctt tattgcgcag gctgcggatg tcataagcga cgtgatccgg gcgaccgaac
    34861 agagaaataa tctggtccat cgtatgcaca ccaaggccat agaacgcgcc atcctgcggc
    34921 aacccaggtt tggtttctgc gaccgggcgg taatagtcaa aatggctttc cacttcaaca
    34981 atctctccca acttgccact ttcaatcgct tttttcgctg tcaggaagca ggagtcaaag
    35041 cgacgattct gatacggcgt gacggtcagc cctttgcttt tcgccaacgc aaacagctcc
    35101 ttcgcctggg caagcgtcgg ggtgaacggt ttttcgacca gcacattttt cccggcttcc
    35161 agcgcgcgtt tcgcgtactc gaaatggctg tccgcgtggg tgcagacaac aaccagctta
    35221 acatcgggat cgtttagtac ttcgtcgaga tcgctggtga aatggatatg ggaataaatg
    35281 ggagcctgtt cttccggctt cgcatggcga cgaaaaatat gcgcgacatg ccagctatcc
    35341 ttgcggttaa gtacatacgg cagatggtaa cgggtggtgc ttttgccgaa gccaataaag
    35401 gcgcagttga tgaccatgat ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa
    35461 gcggacgtgg ttcctactgg agtgcgcata acgaacacaa gcactcccgt ggataaattg
    35521 agaacgaaag atcaaaaaaa aagccagcac ccggctggct aagtaatact ggaagcaatg
    35581 tgagcaatgt cgtgctttca ggttctccgc gagggtcttc ctgatcgcga gacaataata
    35641 atcattctca ttcgcacttg tccaacactt tttgcaaaaa aatgcatttg actcgcattc
    35701 gaaagtcaat gatgttgaaa gggacattta ccccaaagag gacaaaggaa tgagtgagat
    35761 agtgatacgc catgctgaaa cgcgcgatta cgaggccatc aggcagattc acgcccagcc
    35821 ggaggtgtat tacaacacgc tacaagtgcc tcatccttcc gattatatgt ggcaggagcg
    35881 actcgccgat cgtcccggca tcaagcaact cgtcgcctgt attgatggag acgtcgtggg
    35941 ccatctcacc attgacgtgc aacagcgccc acgccgcagt catgttgccg attttggtat
    36001 ctgtgtcgac tcccattgga agaatcgcgg cgtcgccagc gcccttatgc gagagatgat
    36061 tgaaatgtgc gacaactggt tgcgggtaga tcgcattgaa ctaaccgtgt ttgtcgataa
    36121 cgccccggca attaaggtct ataaaaaata cggctttgaa attgaaggga ctggtaagaa
    36181 gtacgcattg cgtaatggtg aatatgtcga tgc
//
LOCUS       DACAPB010000024        35654 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.024, whole genome shotgun sequence.
ACCESSION   DACAPB010000024 DACAPB010000000
VERSION     DACAPB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.024"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(18..93)
                     /locus_tag="IT904_001067"
     tRNA            complement(18..93)
                     /locus_tag="IT904_001067"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="IT904_001068"
     tRNA            complement(152..228)
                     /locus_tag="IT904_001068"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="IT904_001069"
     CDS             complement(331..1716)
                     /locus_tag="IT904_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3190885.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="IT904_001070"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="IT904_001070"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAP3190886.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGHLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="IT904_001071"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="IT904_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAP3190887.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="IT904_001072"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="IT904_001072"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAP3190888.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="IT904_001073"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="IT904_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAP3190889.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="IT904_001074"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="IT904_001074"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAP3190890.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="IT904_001075"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="IT904_001075"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAP3190891.1"
                     /translation="MPVRASIEPLTWENALFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="IT904_001076"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="IT904_001076"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP3190892.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="IT904_001077"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="IT904_001077"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP3190893.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="IT904_001078"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="IT904_001078"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAP3190894.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="IT904_001079"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="IT904_001079"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP3190895.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="IT904_001080"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="IT904_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAP3190896.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="IT904_001081"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="IT904_001081"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAP3190897.1"
                     /translation="MNLLTVSIDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHVSLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="IT904_001082"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="IT904_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP3190898.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="IT904_001083"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="IT904_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAP3190899.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="IT904_001084"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="IT904_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAP3190900.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="IT904_001085"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="IT904_001085"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAP3190901.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18374..19858
                     /gene="gppA"
                     /locus_tag="IT904_001086"
     CDS             18374..19858
                     /gene="gppA"
                     /locus_tag="IT904_001086"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAP3190902.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19905..21926)
                     /gene="rep"
                     /locus_tag="IT904_001087"
     CDS             complement(19905..21926)
                     /gene="rep"
                     /locus_tag="IT904_001087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAP3190903.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGCGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22143..22591
                     /locus_tag="IT904_001088"
                     /pseudo
     CDS             22143..22591
                     /locus_tag="IT904_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22790..23071
                     /gene="ppiC"
                     /locus_tag="IT904_001089"
     CDS             22790..23071
                     /gene="ppiC"
                     /locus_tag="IT904_001089"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAP3190904.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23158..24633)
                     /gene="ilvC"
                     /locus_tag="IT904_001090"
     CDS             complement(23158..24633)
                     /gene="ilvC"
                     /locus_tag="IT904_001090"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAP3190905.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQSKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     INLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNGQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24783..25676
                     /gene="ilvY"
                     /locus_tag="IT904_001091"
     CDS             24783..25676
                     /gene="ilvY"
                     /locus_tag="IT904_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAP3190906.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNHK"
     gene            complement(25728..27272)
                     /gene="ilvA"
                     /locus_tag="IT904_001092"
     CDS             complement(25728..27272)
                     /gene="ilvA"
                     /locus_tag="IT904_001092"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAP3190907.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESSGALLRF
                     LNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27275..29125)
                     /gene="ilvD"
                     /locus_tag="IT904_001093"
     CDS             complement(27275..29125)
                     /gene="ilvD"
                     /locus_tag="IT904_001093"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAP3190908.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29190..30119)
                     /gene="ilvE"
                     /locus_tag="IT904_001094"
     CDS             complement(29190..30119)
                     /gene="ilvE"
                     /locus_tag="IT904_001094"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAP3190909.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30139..30402)
                     /gene="ilvM"
                     /locus_tag="IT904_001095"
     CDS             complement(30139..30402)
                     /gene="ilvM"
                     /locus_tag="IT904_001095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAP3190910.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30399..32045)
                     /locus_tag="IT904_001096"
     CDS             complement(30399..32045)
                     /locus_tag="IT904_001096"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAP3190911.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     NTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32048..32098)
                     /locus_tag="IT904_001097"
     CDS             complement(32048..32098)
                     /locus_tag="IT904_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3190912.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32185..32283)
                     /gene="ilvL"
                     /locus_tag="IT904_001098"
     CDS             complement(32185..32283)
                     /gene="ilvL"
                     /locus_tag="IT904_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAP3190913.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32636..34156
                     /locus_tag="IT904_001099"
     CDS             32636..34156
                     /locus_tag="IT904_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAP3190914.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEHGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34181..34519)
                     /gene="maoP"
                     /locus_tag="IT904_001100"
     CDS             complement(34181..34519)
                     /gene="maoP"
                     /locus_tag="IT904_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAP3190915.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34638..35477
                     /gene="hdfR"
                     /locus_tag="IT904_001101"
     CDS             34638..35477
                     /gene="hdfR"
                     /locus_tag="IT904_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAP3190916.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35573..35648)
                     /locus_tag="IT904_001102"
     tRNA            complement(35573..35648)
                     /locus_tag="IT904_001102"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35613..35615),aa:Trp,seq:cca)
BASE COUNT         8708 a         9709 c         9006 g         8231 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 tgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagataa ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtaatgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattagctc cctgacctca gcattatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agatgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggcggagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgcca ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcgattca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc gagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcagaac ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaggcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttcgccccg cacctttgga
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacctcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacacag
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgctaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaaaaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atctgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgactg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccgct atcactggca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga gggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcacca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accattacct gtcctggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgcgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgccc ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacactcg ccaaatcagg ttcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcatgttggt tgatatcgac accaattacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgcc gttggcagcc cgatataacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca caggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacggc
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 aagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggtacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggta cgcgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaacg tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagc
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagata catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcaatactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacacgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttccgtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcataac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctactggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactatgcca agcagaacca tattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg
    18301 atgagcttac tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg
    18361 aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa
    18421 tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat
    18481 aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctct ccaatgaagc
    18541 aatggagcgc ggttggcaat gtttgcgcct gtttgctgaa cgtctgcaag atattcctcc
    18601 ctcgcaaatt cgcgttgtcg ctacggcgac gttacgcctt gccgtcaatg cgggtgattt
    18661 tattgccaaa gcgcaggaaa ttctcggttg tccggtacag gtgatcagcg gtgaagagga
    18721 agcacgtctg atttatcagg gcgttgctca caccactggt ggtgccgatc agcgcctggt
    18781 ggtggatata ggcggagcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc
    18841 gttgttcagc ctgtcgatgg gctgcgtcac ttggctggaa cgctattttg ccgatcgtaa
    18901 tctggggcag gaaaattttg atgctgcaga aaaagcagca cgcgaagtgt tacgtccggt
    18961 tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca
    19021 ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt
    19081 gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg
    19141 gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt
    19201 taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt
    19261 ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat
    19321 tcaacgccgc tttatgatcg atattgatca ggcacagcgc gtagccaaag ttgcggctaa
    19381 cttcttcgat caggtggaaa acgaatggca tcttgaagca ataagccgcg atttgctcat
    19441 cagcgcctgt cagcttcatg aaatcggcct gagcgttgac ttcaaacaag cgccgcaaca
    19501 cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt
    19561 gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca
    19621 aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt ttactacgcc tggccatcat
    19681 ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga
    19741 actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat
    19801 tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg
    19861 agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt
    19921 gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg
    19981 gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa
    20041 aagcggctcg gctccgggcg caccagttcg ccgtactgac ggcgttcttt acacagcgta
    20101 aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg
    20161 atattatctt catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg
    20221 tagacataag gaaactccag ccctttcgac gcgtggagag tcatcagttg cacctgatcc
    20281 agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc
    20341 tgggtgagcg tcatcggctc atccagttca ctgccttcca gcatctccgt catccagcta
    20401 aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg
    20461 tacagccagg attcataatc catgccatgg atcagatcac gcaccgcggc aatcggctcc
    20521 cgctccgcca gacgctggat ttctgccaac cagtgagtga agcgggtcaa tgcttcataa
    20581 ccacatccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta
    20641 ttgcgcgtca tcgcccactc acccagcttt ttcagcgtag ccgggccaat ctctcgcttc
    20701 ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga
    20761 taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc agatattttg
    20821 tacgggatgc ggttttgcat caggaatttt tcaaacaccc gcgactgatg gttaccgcga
    20881 taaagaatgg cgtaatcttt gtactgcgtt ttattgacga agtgatgggc gatcagctcg
    20941 ccagtaacgc gctcagcctc atgttcttcg ttattcgcgc ttaatacttt tagctccgcg
    21001 ccataaccca gttcggagaa cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg
    21061 atgttcgccg ctttcagaat acgcccggaa gagcgatagt tctgctcaag cttaatcacc
    21121 ttcagcgccg gaaaatcctg actcagcagc accaggtttt gcggacgtgc accgcgccag
    21181 gagtagatcg actggtcatc gtcacccacc acggtaaagc gcgcgcggct gcccaccagc
    21241 agtttcacca gctcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag
    21301 cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagcag caacgtcggc
    21361 aataaaatca gatcatcgaa gtcgagaacg ttacaggctt tcaggtgtgc atcatacagc
    21421 ccataacaat gggcaaaaat acggtcccgc tcgccaatcg cacttgctgc cgcctgggac
    21481 ggtgttttga gatcattctt ccagttagag atggtcgaaa tcagttgttg caggagaact
    21541 ttgtcatctt caatcagccc ctcggtcaac tctttaagca aagcaagctg atcggtatcg
    21601 tcaaacaacg agaagttcgc tttcatccca agcgccgcat actcgcgttt gatgatatcc
    21661 agccccaacg tatggaaagt ggagatcatc agcccacgcg cctctttgcg ccccagcgtc
    21721 tgccctacac gctctttcat ctcgcgcgct gctttattag taaaggtcac cgccgcaatg
    21781 tgccgcgcct gataaccgca accgcggatc agatgggcga ttttattggt gataacacga
    21841 gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct
    21901 tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga
    21961 ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt tggctgcgga
    22021 tgtttaaatc agagagagaa gaccgaaatc ggagttcatc gtgaatccat tagacttaga
    22081 aaatatcggg tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtttac
    22141 tcgtggcgat taatttcagc cctaaggttg gtgaaatact ggaatgcaac tttggtaact
    22201 atcctgttag ccaaaatgga ccgttcagca cgacgtatta cgatggccgc attcctcctg
    22261 aaatgataaa aaacaggtta gttgtcgtgc tcaacggaaa aattaatggc aacgcatgca
    22321 tcgtcgttcc attatcaacc actcgcgacc agacaagtta aaacgtggaa tgcatgttga
    22381 aatagccagt aatgttatca atgacctgca attctttgat cagcagattc gttgggccaa
    22441 agccgacctg gtacaacaag tcagtcgaaa cagattaaac agagcaagga cttacagagg
    22501 gtatcttaat caatgtttgc cacatgagtt ggttgcagac atacagaggg cggtcatcaa
    22561 gtcaatcaat gccatatcgc tcataaattg acaataaaat aaccatcccc gataatgact
    22621 ttgtagccca tagaggccct aagttttctc cccttgaggg acctgcgaag cccggatttt
    22681 ccgggctttt gccatatatg taccagccac tccctgactg acaattacgt tagcatctgc
    22741 ggatcatgct aaaatcgccg cctgacaatt attcactcaa ggcacgatca tggcaaagac
    22801 agcagcagca ctgcatatcc ttgtaaaaga agagaaactg gctctggatc ttctcgagca
    22861 gattaagaac ggggccgatt tcggcaagct ggcgaagaaa cactccattt gcccatcagg
    22921 caaacgcggc ggtgatttag gtgaattccg ccagggtcag atggttccgg cgttcgataa
    22981 agtggttttc tcttgtccgg tactggagcc gaccggcccg ctgcacaccc agttcggata
    23041 tcacatcatt aaggtgctgt accgcaacta atagcaaggc cttctccagg agaaggcctt
    23101 gagtgttttc tccctctccc tgtgggagag ggtcggggtg agggcatcag cgcgcactta
    23161 acccgcaaca gcaatacgtt tcatatctgt catatagccg cgcagtttct tacctacctg
    23221 ctcaatcgca tggctgcgaa tcgcttcgtt cacatcacgc agttgcccgt tatctaccgc
    23281 gccttccgga atagctttac ccaggtcgcc cggttgcagc tctgccataa acggtttcag
    23341 caacggcaca caagcgtaag agaatagata gttaccgtac tcagcggtat cagagataac
    23401 cacgttcatt tcgtacagac gcttacgggc gatggtgttg gcaatcagcg gcagctcgtg
    23461 cagtgattca taatatgcag actcttcaat gatgccggaa tcgaccatgg tttcgaacgc
    23521 cagttcaacg cccgctttca ccatcgcgat catcagtacg cctttatcga agtactcctg
    23581 ctcgccgatt ttgccttcat actgcggcgc ggtttcaaac gcggttttgc cggtctcttc
    23641 acgccaggtc agcagtttct tatcatcgtt ggcccagtcc gccatcatac cggaagagaa
    23701 ttcgccggag atgatgtcgt ccatatgttt ctggaacagg ggtgccatga tctctttcag
    23761 ctgttcagaa agcgcataag cacgcagttt cgccgggtta gagagacggt ccatcatcag
    23821 ggtgatgccg ccctgtttca gtgcttcggt gatggtttcc caaccgaact gaatcagttt
    23881 ttctgcgtat gctggatcgg taccttcttc caccagcttg tcgaagcaca gcagagagcc
    23941 agcctgcaac ataccgcaca ggatggtttg ctcgcccatc aggtcagatt tcacttccgc
    24001 aacgaaggac gattccagca cacccgcacg gtgaccaccg gttgcagccg cccaggcttt
    24061 ggcaatcgcc atgccttcgc ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg
    24121 tacgccgaac ccacgtttgt actcttcacg cacttcggtg cctgggcatt tcggcgcaac
    24181 catcactacg gtgatatctt tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg
    24241 cgagtagccc agcgccgcgc cgtctttcat cagtggctgt acggtgcgca ctacatcaga
    24301 gtgctgcttg tccggcgtca ggttaatcac cagatccgcc tgtgggatca gttcttcgta
    24361 agtacccact ttaaaaccat tttcggtcgc tttacgccag gacgcgcgct tctcggcaat
    24421 cgcttcttta cgcagagcgt aggagatatc gagaccagaa tcacgcatgt tcaggccctg
    24481 gttcagaccc tgtgcgccac agccgacgat gactactttt ttactctgaa ggtagctcgc
    24541 gccatcggcg aattcatcgc ggcccataaa gcgacatttg cccagctgtg ccagctgctg
    24601 gcgcagattc agtgtattga agtagttagc catggtgatt cctcgtgatg ttgtgcttct
    24661 tattgttcgg ttcgcgtttg cgagattgaa ttcactatat gacaggaaat ttattgcgga
    24721 aattgatata ttcacaacgt cacattgcaa tttttgcaac gtcaacatcg agggctgtcc
    24781 ctgtggattt acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc cattttggcc
    24841 gcagcgcgcg ggcgatgcac gttagcccat ccacgctctc acggcagatt cagcgcctgg
    24901 aagaagatct cggtcagccg ctgtttgtgc gcgataaccg cacggtgacg ctgactgaag
    24961 cgggcgaaga gctgcgcgtt ttcgcccagc aaacgctgtt gcagtatcag cagttgcgcc
    25021 acaccatcga tcagcaaggg ccgtcgctct ctggcgaatt acatatcttc tgctcggtga
    25081 ccgctgccta cagccatctg ccgccgattc tggatcgctt ccgcgcggaa cacccgtcgg
    25141 tggagattaa acttactact ggtgatgcgg cagatgcgat ggaaaaggtg gtcactggtg
    25201 aagcggatct ggcgattgcg ggtaaaccgg aaaccttgcc cggcgcagtg gcgttttcga
    25261 tgttggagaa tctggcagta gtgctgattg cccccgcgct gccctgcccg gtgcgtaatc
    25321 aggtgtcggt agagaagccg gactggtcaa cggtgccgtt tattatggcc gatcaggggc
    25381 cggtacgccg ccgcattgaa ctgtggtttc gacgcaataa aatcagtaac ccgatgattt
    25441 acgccacggt tggcgggcat gaagcgatgg tatcgatggt ggcactcggc tgtggcgtgg
    25501 cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc ggtgcgtaac cgcgtgatga
    25561 ttttagagcg cagcgatgag aaaacgccgt ttgagctggg cgtgtgtgcg cagaaaaagc
    25621 ggctacatga gccgctaatt gaggcattct ggaagatttt gccgaaccac aaatgacgtt
    25681 gtcgcgcggg taggcctgat aagcgaagcg ctatcaggca tttttcccta acccgccaaa
    25741 aagaacctga acgccgggtt attggtttcg tcgtggcaat cgtagcccag ctcattcagc
    25801 cgggtttcga aatccggttc atggtcgcca agttcgaacg ccgccagtac gcgcccgtag
    25861 tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtacgtacc cagcgtgttg
    25921 aggaagcgca gcagcgcgcc cgatgattcc gggaattcga agctgtagag gcgttcctgc
    25981 aacggatgcg atggacgtcc gccgaccata tagcgcacgt gtagcttcgc catttcgtcg
    26041 tcggagagat caaccacgct gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc
    26101 tcttcgaggc cgcggctcag gcgcacaccg acaaagatgc aggcgttttt ggcatcggca
    26161 aaacggtagt tgaactcggt gaccgaacgc ccgccaagca gttggcagaa tttgaggaag
    26221 ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg
    26281 cagcgttctg agacgtagcg caggccgtgg aagttcacgt tggcaccgga aagaatatgc
    26341 gccagccgtt cgccgcgaat gttgtgcagg gcgatatatt ttttcattcc cgccagcgcc
    26401 agcgcgccag agggttccgc caccgcgcgc acatcttcga ataaatcctt catcgccgca
    26461 cagatcgcat cgctatcgac ggtgatgatg tcgtcgagat actcctggca taaacggaag
    26521 gtttcgtcac cgatgcgttt taccgctacg ccttcagcaa atagccctac gcgcggcaga
    26581 tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc cgcttctacg
    26641 gcgatcactt tgatttgcgg catcagttgt ttgatcagca ccgccacgcc agcagccaga
    26701 ccgccgccgc cgactggcac aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc
    26761 agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg cacccaggtg
    26821 aacccctgct gctgtgacag ttcgatcgct ttggctttcg cttcatcaaa gttcgcaccg
    26881 tggagcagca cttcgccgcc gaagccgcgc accgcatcaa ctttgatatc ggcggtggcg
    26941 gttggcatga cgatcagtgc cttcacgcct aaccgtgcgg aagaaaacgc gacgccctgc
    27001 gcgtggttac ccgcagaagc ggtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc
    27061 gccatcatgg cgtatgcacc gcgcagctta aagctgtgca ccggctggcg atcttcgcgc
    27121 ttcaccagaa tcacgttatc aagacgcgac gacagttttt ccattttctg tagcggcgta
    27181 acctgcgccg cttcatagac cggcgcgcgt agcaccgctc ttaaatattc ggcaccttcc
    27241 ggggtgccgg acaggggttg cgagtcagcc atcattaacc ccccagtttc gatttatcgc
    27301 gcaccgcacc tttgtcggcg ctggtcgcca ggctggcgta ggcacgcagc gcaaaggaaa
    27361 cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgggcttcc tgcgcttcac
    27421 gacgcgccgc cagttcggca tcgcttacct gtaactgaat accacggttc ggaatgtcga
    27481 tagcgataag atcgccgtct tcaatcaggc caatgctgcc gccgcttgcc gcttccggtg
    27541 agacgtgccc gatagaaagg ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg
    27601 cacaggcttt accgagcccc attgatttca ggaagctggt tgggtagagc atttcctgca
    27661 tccccggacc gcctttcggg ccttcatagc gaattactac cacatctccg gcgacaactt
    27721 taccgccgag aatcgcttct accgcgtcgt cctggctttc gtacactttc gccgggccgg
    27781 tgaatttgag gatgctgtca tcgacgcccg cggttttaac gatgcagccg ttttctgcga
    27841 aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga
    27901 tacagccgtt tgctcgatcg tcatcgagag aatcccaacg gcaatcctgc gagaatgcct
    27961 gtgtagtccg aatgcccgcc gggcctgcgc ggaacatatt ttttaccgcg tcatcctggg
    28021 tcagcataac gtcgtattgt tccagcgttt gcggcaacgt caggccaagt acgtttttca
    28081 catcacggtt cagtaacccc gcacgatcca gttcgccgag aataccgata acaccaccag
    28141 cacgatgaac atcttccata tggtatttct gggtgctcgg cgcaacttta cacagctgcg
    28201 gaaccttgcg ggagagctta tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg
    28261 ccgccgccag caggtgaagt acggtgttag tcgatccacc catcgcgata tccagcgtca
    28321 tggcgttttc aaacgccgcc ttactggcga tattacgcgg cagtgcactt tcgtcgtttt
    28381 gctcgtaata acgtttggtc aattcaacaa tgcgtttacc agcattaagg aacagctgct
    28441 tacggtccgc gtgggttgcc agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt
    28501 cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggaaccg caggttgggc
    28561 aggcggaacg ttcaacctga tcgctctggg agtcagagac tttcgggtct gcgccctgga
    28621 tcatcgcatc aaccagatcg agcttgatta tccgatcgga cagcttggtt ttcccggctt
    28681 ccatcgggcc gccggaaaca aagatcaccg gaatatttaa tcgcagggaa gccatcaaca
    28741 tcccaggggt gattttgtcg cagttggaga tacagaccat ggcatcggcg cagtgggcgt
    28801 tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc
    28861 ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct ttggcaacgc
    28921 cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga tggacgtgac
    28981 ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttaccg aaatcggcgt
    29041 cggtcatccc ggtggcgcgc cacagtgcgc gggcccccgc catattacgg ccatgggtgg
    29101 tggtagcgga acgatactta ggcatgcttt atttactccc agtgtctgtc tcgtaaatgg
    29161 gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa ccagccccat
    29221 ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctggatgcg tttggtaacc
    29281 gggccgcaac ggccttcacc aacctgaata ccatctacgc tgcgcactgg cgtgatttct
    29341 gccgcagtac cggacataaa cacttcatcc gccaggtaca gggattcgcg cgacagcacc
    29401 tgctcacgga cttcaattcc cagctctttt gccagtttga tgatggcatc acgggtaata
    29461 cccggcagcg cggaggaggt gaacggtggg gtgaacagca cgccgtcttt cacttcaaac
    29521 aggttttcgc ctgcaccttc agagatgtaa ccattcacat ccagcgcgat accttcctga
    29581 taaccgtggc ggcgtgcttc actacccacc agcagggaag agaggtagtt accaccggct
    29641 tttgccgcgg ttgggatggt gtttggtgct gcgcggttcc aggaggaaac catcgcatcg
    29701 atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc ggcgataatc
    29761 acatcggttg agtatcccgc tggcgggtta acgcccatgc caacatcacc gacgaagatc
    29821 agcggacgga tataggcgct ggtgagattg tttttgcgga tcacgtcacg acaagcttcc
    29881 atcagctcat caatgctctg cgaaaccgga aagcgataga ttttggcgga gtcatgcaga
    29941 cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgcgagtc gtagcaacgg
    30001 atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg caccttcgcg
    30061 tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt cgtggtcatt
    30121 tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc tctggcagat
    30181 ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt cgaccgaccg
    30241 tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg cggccatatt
    30301 cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc
    30361 tggattgaag cgagccgata cattgacctg atgttgcatc atgataattt ctccaacatt
    30421 tctgaattac tggcacctgg cggcaccagc ggccagacgt tctcaagttc gtcgattgag
    30481 acatgaagca ggtatggccc atcactgttc agcatggtgt tgagtgccgc ttcaacctgg
    30541 tctttacggg tgatgtgttg gccagggatg ccgaaggcgc tggctaacat gaggaaatcg
    30601 gggttatcag taagggtggt ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt
    30661 cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt
    30721 tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga gatacagaca
    30781 acggtatcgt tcggtcgcgc gacttgtgcg ccaaccgccg ccggtaaacc aaaacccatg
    30841 gtacctaagc cgctggaggt gatgaaattt tccgggcgag tgtgggcgat gtgctgcgca
    30901 gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg tttacgatcc
    30961 gacagttgtt ttaacaacaa cggcgcgtag atagcgtcac cgggatggtc gtaacgccag
    31021 gcatgttcat cacgcagctg cgcgcagtgt tgctgccagt cattgatatt taacggctgc
    31081 tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc
    31141 ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg tgcgaaggtg
    31201 ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac
    31261 tcctgcaccg cgaagttcgc cgctttggtg ccatgcattc ccagcatgcc cagatagtac
    31321 ggataatctg cttcaactgc gcccagccct ttcagcgtgc aggtggcagg catttttgtg
    31381 gtagcgagaa attctcgtaa agcaggaact gcctgcgcca tacccacgcc accaccaacg
    31441 tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcgac ttcggcatgt
    31501 gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccaggtcgcc gctggctaat
    31561 tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct ggcaacgtcg
    31621 aatgcttcag ccatgatgcg cggcaactct tccagcgact gcaccaggaa gctgtgcttg
    31681 gtacaggcta gcgacaatcc caggacatcc acttcctgaa atgcgtccgt gccgataaac
    31741 ggtgcggaca cttgaccggt gatggcaaca acggggatgg aatctaacag tgcgtccgca
    31801 agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac gccagttttg
    31861 ccagtagcac gggcataacc gatagccgcc attgccgcac cctgttcatg tcggcacagc
    31921 aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc gccacccgga
    31981 taaccgaaaa cggtattcac accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca
    32041 ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg ttattcattg
    32101 tctgctcctc ggttatgttt ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg
    32161 tcttagttcg ttaaggcttg atttctaagc ctttcctcgt ccaagtgcag ccccgcacgg
    32221 tgggataata atcaccacca cgctaatcac gaccaggcta atcactcgta gaagggctgt
    32281 cattttgtct tttcttgcat cttgttcgaa ggaatgctta aagagttacc ataggttttg
    32341 taaatagtac aagatatttt ttggccgatt tttatgatgc gtttaataat ttcaatagaa
    32401 attgttgttt tttaaaaaga aaatagtgag tgaaaaaaat tcaatttcgg tctgaatgtt
    32461 gttcccttcc tcttagttca tcctttgtcc gctgtatttt tgcgagcatc attccaccgc
    32521 cagaacaact caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat
    32581 tagcgggcta cagcagcgat atcttatgca cataatggtg actcaaggag ggcttatgtc
    32641 actgtcaatt gttcataccc gcgcagccct gggagtaaat gcgcccccaa tcactgttga
    32701 ggtacatatc agtaaaggtc tacccggctt aacgatggtg ggcttaccag aaacaacggt
    32761 gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg aatatccggc
    32821 gaaaaaaatc accatcaacc ttgctccagc cgatctacca aaagaagggg gacgatatga
    32881 tttacctatc gccattgcgt tgctggcggc ctcagaacag cttacagcca ataagttaga
    32941 tgaatatgaa ttagtcggag aactggcgct tacaggcgct ctgcgtggcg ttcccggcgc
    33001 aatctccagt gcaactgaag ctattaagtc gggcagaaaa attatcgtcg cgaaagataa
    33061 cgaagatgaa gtggggctaa ttaacggtga aggatgcctg atagccgatc atctgcaagc
    33121 tgtctgtgcg tttctggaag gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt
    33181 atcccgggcg ctacaacatg atctcagtga tgttgtcggt caggaacatg gaaagcgagg
    33241 actggaaatt accgccgctg gcgggcacaa ccttttactg attgggcctc cgggaacagg
    33301 taaaacaatg ctcgccagcc gtattaatgg cctgttgcca gatttaagca atgaagaggc
    33361 actggagagt gctgcgatat taagtctggt aaatgctgaa tcagtacaaa aacaatggcg
    33421 gcagcgcccg ttccgctcac ctcatcacag tgcatcgtta actgcgatgg taggcggtgg
    33481 cgcaattcca gggcccggtg aaatttcgct ggcgcataac ggcgtgcttt ttcttgatga
    33541 gttacctgaa tttgaaaggc gtacactgga tgccttgcga gagccgattg aatccgggca
    33601 gatccatctt tcacgcacgc gagcaaaaat aacctatcca gcccgtttcc agcttgtcgc
    33661 ggcgatgaat cccagcccta ccggacatta tcagggaaac cataaccgct gcacaccaga
    33721 gcaaacgctg cgttatctca accgactctc tggtcccttt ctcgaccgct tcgatctctc
    33781 actggagatc ccattaccgc ctcccggtat tttgagtaaa acggtagtgc cgggagaaag
    33841 cagcgccacc gttaaacaac gcgtaatggc cgccagagag cgccaattta agcggcagaa
    33901 taaactgaac gcctggctgg atagtccgga aatacgccaa ttctgcaaac ttgagagcga
    33961 agatgcgatg tggctggaag aaacgctgat ccatctgggg ttatcgattc gtgcctggca
    34021 gcggttattg aaagttgcac gaaccattgc tgatattgat cagtctgaca ttatcacacg
    34081 tcaacatttg caggaggcag ttagctatcg agcgattgac cgtttgctca tccatctgca
    34141 gaaactactg acataaaaaa agggcatttc gcccttttta ttaatcgtca gaatcggtgt
    34201 agtcttcagc accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac
    34261 gcttaatacg cgtcatatac ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt
    34321 cgccacggca tactgctacg aagagtttct cttcctcggt aaccggctcg cgtttgccaa
    34381 gatccaactc attgaaggca taaccatgac gctcaagcag ttgtgcctct ttgatggtga
    34441 aatcaccatg acgagagaat ccacgtggat aatgtttatt gtcgaaatat cgattagtcg
    34501 tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt gaccgagcta gttatggcgc
    34561 ggagtattag ttacgcttga cagagtgtaa aacaaaacat ttaaatcata acgacaaata
    34621 attttgcgga gagcactgtg gatacggaat tgttaaaaac tttcctggaa gttagccgaa
    34681 cgcgtcactt tggtcgagcg gctgaatcgc tctatctgac ccagtcagca gtgagctttc
    34741 gaatcagaca actggaaaat caactgggtg tgaacctttt cacccgccac agaaacaata
    34801 tccgtttaac cgctgccggt gaaaaactac tgccttatgc agaaacgctc atgagcacct
    34861 ggcaggccgc ccgcaaggag gtggcgcata cctcacgaca taacgagttt tctatcggtg
    34921 ccagcgcctc attgtgggaa tgtatgctta atcagtggct gggacgtttg tatcaaaatc
    34981 aggatgccca tacaggctta cagttcgaag cgcgaattgc ccaacggcag tctctggtaa
    35041 aacagctgca tgaacgccag cttgatcttc ttattaccac tgaagcgccc aaaatggacg
    35101 aatttagtag tcagttgctg ggatatttca ctttagcgct ttataccagt gccccttcaa
    35161 aactaaaggg agatcttaat tatctgcgac ttgagtgggg gccagatttt caacagcatg
    35221 aggcaggttt gatcggtgct gacgaagtgc ccattctgac aaccagttct gctgaactgg
    35281 cacagcaaca gattgcgatg cttaatggtt gcacctggct acccgtcagc tgggcgcgta
    35341 aaaaaggcgg cctgcatacc gttgtcgata gcacaacact ttcacggccg ctttatgcca
    35401 tatggctgca aaatagcgat aaaaatgcgt tgattcgcga tcttttaaaa attaacgtgc
    35461 tggatgaagt gtattaatat gaatggctgg caaggatgcc ggtagaagga tttacttcgg
    35521 agagggttat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggcaggg
    35581 gcggagagac tcgaactccc aacacccggt tttggagacc ggtgctctac caattgaact
    35641 acgcccctaa ttag
//
LOCUS       DACAPB010000025        35527 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.025, whole genome shotgun sequence.
ACCESSION   DACAPB010000025 DACAPB010000000
VERSION     DACAPB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.025"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            133..741
                     /locus_tag="IT904_001103"
     CDS             133..741
                     /locus_tag="IT904_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAP3190917.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            748..1371
                     /locus_tag="IT904_001104"
     CDS             748..1371
                     /locus_tag="IT904_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HAP3190918.1"
                     /translation="MKKIIPAVVCLLLCSCSTFPQAVAPVNNDFAGQHNIYIVSHGWH
                     TGIVVPAAIVNRVLPQLDARFAQPKWYEIGWGDKGFYQAQEITSRLTLQAMFWSTGAV
                     MHVVAFSAPPERYFPGSEVKPLTINNGQLATLMLFIGRSFSRSEAGNVIPLKKGIYGD
                     SQFYAANGRYGILNTCNKWTAKGLQSAGIEIEPALKLTAGSVISAMP"
     gene            complement(1426..1638)
                     /gene="rpmE"
                     /locus_tag="IT904_001105"
     CDS             complement(1426..1638)
                     /gene="rpmE"
                     /locus_tag="IT904_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAP3190919.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1841..4039
                     /gene="priA"
                     /locus_tag="IT904_001106"
     CDS             1841..4039
                     /gene="priA"
                     /locus_tag="IT904_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP3190920.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            4195..5220
                     /gene="cytR"
                     /locus_tag="IT904_001107"
     CDS             4195..5220
                     /gene="cytR"
                     /locus_tag="IT904_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAP3190921.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            5312..6271
                     /gene="ftsN"
                     /locus_tag="IT904_001108"
     CDS             5312..6271
                     /gene="ftsN"
                     /locus_tag="IT904_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAP3190922.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6364..6894
                     /gene="hslV"
                     /locus_tag="IT904_001109"
     CDS             6364..6894
                     /gene="hslV"
                     /locus_tag="IT904_001109"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAP3190923.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6904..8235
                     /gene="hslU"
                     /locus_tag="IT904_001110"
     CDS             6904..8235
                     /gene="hslU"
                     /locus_tag="IT904_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAP3190924.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            8302..9228
                     /gene="menA"
                     /locus_tag="IT904_001111"
     CDS             8302..9228
                     /gene="menA"
                     /locus_tag="IT904_001111"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP3190925.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            9321..9806
                     /gene="rraA"
                     /locus_tag="IT904_001112"
     CDS             9321..9806
                     /gene="rraA"
                     /locus_tag="IT904_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAP3190926.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9891..10136)
                     /gene="zapB"
                     /locus_tag="IT904_001113"
     CDS             complement(9891..10136)
                     /gene="zapB"
                     /locus_tag="IT904_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAP3190927.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10561..11406
                     /gene="glpF"
                     /locus_tag="IT904_001114"
     CDS             10561..11406
                     /gene="glpF"
                     /locus_tag="IT904_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAP3190928.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFDQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11429..12937
                     /gene="glpK"
                     /locus_tag="IT904_001115"
     CDS             11429..12937
                     /gene="glpK"
                     /locus_tag="IT904_001115"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP3190929.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13072..14082
                     /gene="glpX"
                     /locus_tag="IT904_001116"
     CDS             13072..14082
                     /gene="glpX"
                     /locus_tag="IT904_001116"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP3190930.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14179..14925
                     /gene="fpr"
                     /locus_tag="IT904_001117"
     CDS             14179..14925
                     /gene="fpr"
                     /locus_tag="IT904_001117"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAP3190931.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14930..15358)
                     /gene="uspD"
                     /locus_tag="IT904_001118"
     CDS             complement(14930..15358)
                     /gene="uspD"
                     /locus_tag="IT904_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAP3190932.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(15385..15684)
                     /locus_tag="IT904_001119"
     CDS             complement(15385..15684)
                     /locus_tag="IT904_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAP3190933.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAATSSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15896..16336)
                     /locus_tag="IT904_001120"
     CDS             complement(15896..16336)
                     /locus_tag="IT904_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP3190934.1"
                     /translation="MTIQQWLFSFKGRIGHRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            16437..17036
                     /locus_tag="IT904_001121"
     CDS             16437..17036
                     /locus_tag="IT904_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAP3190935.1"
                     /translation="MKPGCTLFFLLCSALTVTTAAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            17144..17911
                     /gene="tpiA"
                     /locus_tag="IT904_001122"
     CDS             17144..17911
                     /gene="tpiA"
                     /locus_tag="IT904_001122"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP3190936.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17966..18721)
                     /gene="cdh"
                     /locus_tag="IT904_001123"
     CDS             complement(17966..18721)
                     /gene="cdh"
                     /locus_tag="IT904_001123"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAP3190937.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFIMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18828..19817)
                     /locus_tag="IT904_001124"
     CDS             complement(18828..19817)
                     /locus_tag="IT904_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3190938.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(20137..21099)
                     /gene="pfkA"
                     /locus_tag="IT904_001125"
     CDS             complement(20137..21099)
                     /gene="pfkA"
                     /locus_tag="IT904_001125"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP3190939.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(21280..22182)
                     /gene="fieF"
                     /locus_tag="IT904_001126"
     CDS             complement(21280..22182)
                     /gene="fieF"
                     /locus_tag="IT904_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAP3190940.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(22331..22831)
                     /gene="cpxP"
                     /locus_tag="IT904_001127"
     CDS             complement(22331..22831)
                     /gene="cpxP"
                     /locus_tag="IT904_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAP3190941.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22981..23679
                     /gene="cpxR"
                     /locus_tag="IT904_001128"
     CDS             22981..23679
                     /gene="cpxR"
                     /locus_tag="IT904_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAP3190942.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23676..25049
                     /gene="cpxA"
                     /locus_tag="IT904_001129"
     CDS             23676..25049
                     /gene="cpxA"
                     /locus_tag="IT904_001129"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAP3190943.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(25155..25829)
                     /gene="yiiM"
                     /locus_tag="IT904_001130"
     CDS             complement(25155..25829)
                     /gene="yiiM"
                     /locus_tag="IT904_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAP3190944.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25978..26961)
                     /gene="kdgT"
                     /locus_tag="IT904_001131"
     CDS             complement(25978..26961)
                     /gene="kdgT"
                     /locus_tag="IT904_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAP3190945.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(27221..27841)
                     /gene="sodA"
                     /locus_tag="IT904_001132"
     CDS             complement(27221..27841)
                     /gene="sodA"
                     /locus_tag="IT904_001132"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAP3190946.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            28126..29160
                     /gene="rhaT"
                     /locus_tag="IT904_001133"
     CDS             28126..29160
                     /gene="rhaT"
                     /locus_tag="IT904_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAP3190947.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKSLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(29157..30095)
                     /gene="rhaR"
                     /locus_tag="IT904_001134"
     CDS             complement(29157..30095)
                     /gene="rhaR"
                     /locus_tag="IT904_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAP3190948.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(30079..30915)
                     /gene="rhaS"
                     /locus_tag="IT904_001135"
     CDS             complement(30079..30915)
                     /gene="rhaS"
                     /locus_tag="IT904_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAP3190949.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            31203..32672
                     /gene="rhaB"
                     /locus_tag="IT904_001136"
     CDS             31203..32672
                     /gene="rhaB"
                     /locus_tag="IT904_001136"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP3190950.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQEQQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLYIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            32669..33928
                     /gene="rhaA"
                     /locus_tag="IT904_001137"
     CDS             32669..33928
                     /gene="rhaA"
                     /locus_tag="IT904_001137"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP3190951.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
     gene            34011..34835
                     /gene="rhaD"
                     /locus_tag="IT904_001138"
     CDS             34011..34835
                     /gene="rhaD"
                     /locus_tag="IT904_001138"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP3190952.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34845..35159
                     /gene="rhaM"
                     /locus_tag="IT904_001139"
     CDS             34845..35159
                     /gene="rhaM"
                     /locus_tag="IT904_001139"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP3190953.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35460..>35527
                     /locus_tag="IT904_001140"
     CDS             35460..>35527
                     /locus_tag="IT904_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP3190954.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT         8713 a         9214 c         9175 g         8425 t
ORIGIN      
        1 ggcctacgtc atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt
       61 cacgccgcat ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat
      121 ggacgtttaa ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc
      181 tttgcctggc agccacaaac cggcgacatc atctttcaga tctctcgctc atcgcaaagt
      241 aaagcgatcc aactggcgac ccattccgat tatagccaca ccggtatgct ggtgatacgc
      301 aacaaaaagc cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag
      361 tggatcgccc atggtgaaaa gggcaaatac gttgttcgcc gcgttgaagg cggactgagt
      421 gttgaacaac agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat
      481 ttcagcttct catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac
      541 cagaacgcgc tgggaatgcg cgtgggcgag cagcagaagt tgaaagagtt cgatctcagc
      601 aacccgctgg tccaggctaa gctcaaagaa cgctacggca aaaatattcc gcttgaggaa
      661 acggtagttt caccacaagc cgttttcgac gcgccacaac tcaccacagt cgccaaagag
      721 tggccgctgt tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg
      781 ttgctttgta gttgctcgac ctttccgcaa gctgtcgctc cggtaaacaa tgactttgca
      841 ggtcagcata atatttatat tgtcagtcac ggctggcata ccggaattgt ggtgcccgcc
      901 gcaatcgtta atcgtgtttt accgcaactc gacgcgagat ttgcgcaacc aaaatggtat
      961 gaaatcggct ggggcgataa aggcttttat caggcacagg aaattaccag tcgcctgaca
     1021 ttgcaggcga tgttctggtc tactggcgca gtaatgcatg tggtggcgtt ctctgcccca
     1081 cctgaacgct attttcctgg cagtgaagtt aagccactga caattaacaa cggtcaactt
     1141 gcgaccttaa tgctatttat tggccgcagt ttttcccgta gcgaggcggg aaatgttatt
     1201 ccactcaaaa aaggcattta tggtgacagc cagttttatg ccgccaatgg tcgctatggg
     1261 attttaaata cctgtaataa atggacggca aaagggttac aaagcgcggg gatagagatt
     1321 gaaccggctt taaaattgac ggcggggagt gtgataagcg caatgcctta acgattgtca
     1381 ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc cggtcttatt tgctgcccgg
     1441 gatgttgaaa cgcttgttga agcggtcaac acggccaccg gtagcaacat cacgctgttt
     1501 gccagtgaag aatgggtggc acttgctgca cacgtcgagg ttcaggtcat gaccaacggt
     1561 ggagcggatt ttcattacgt taccgcaaga gcagctagca gtaatttctt cgtatttcgg
     1621 gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc gcgtcgctct tccagcccta
     1681 acgccagaca ccacgcgatg ttaaaagtat agcttcaata cgatcatttc gtacgaaggc
     1741 gcgaaatcat acagaaatta accagcgtat gcaaactgat ccgcactctt ctacggcaat
     1801 gtgtatacta acccaccgaa tttcaagtca ggatgatgct atgcccgttg cccacgttgc
     1861 cttgcccgtt ccgcttcctc gtacctttga ctatctgctg ccagaaggca tgacggttaa
     1921 agccgggtgt cgcgtgcgcg tgccgtttgg caaacagcag gagcgcatcg gggttgtggt
     1981 gtcagttagc gatgtcagtg agctgccgct taacgagctg aaagcggtag tggaagtgct
     2041 ggacgttgag ccggtattta ctcactccgt ctggcggtta ctgctctggg cggcagatta
     2101 ctatcatcat ccgattggcg atgtgctgtt tcatgccttg ccgattttac tgcgccaggg
     2161 gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc actgaacaag gccaggcggt
     2221 ggatctgaac agcctgaaac gctcccccaa gcaacaacag gcgctggcgg cgttacggca
     2281 aggcaaaatc tggcgcgacc aggtcgccac gctcgaattt aatgatgccg cgttgcaggc
     2341 gctacgcaaa aaaggtctgt gtgatttagc aagtgaaaca ccagagttta gcgactggcg
     2401 aacgaactat gccgtttctg gtgagcggtt gcgattgaat accgaacagg ccaccgccgt
     2461 tggcgcaatt catagcgcgg cagatacttt ttctgcctgg ctgctggcgg gcgttaccgg
     2521 ttccggtaaa acggaggttt atctcagcgt actggaaaac gtgctcgctc agggcaaaca
     2581 ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa actatcgccc gttttcgtga
     2641 acgttttaat gcccccgtgg aagttctgca ttccggcctg aacgacagcg agcgtctttc
     2701 ggcgtggctg aaagcgaaaa atggtgaggc ggcgattgtg atcggcaccc gctccgcgct
     2761 gtttacgccg tttaaaaatc tcggcgtgat tgtcattgat gaagagcacg acagctccta
     2821 caagcagcag gaaggctggc gctatcatgc ccgcgacctg gcggtgtatc gtgcgcacag
     2881 cgagcaaatc ccgattattc ttggctccgc aacgcccgcg ctggaaacgt tatgcaacgt
     2941 ccagcagaaa aaataccgcc tgctgcgcct gacccgtcgg gcagggaatg cgcgtccggc
     3001 aattcaacat gtgctggatt taaaaggtca gaaggtgcag gcaggtctgg ctccggcgtt
     3061 aatcactcgt atgcgccagc atttacaggc tgataaccag gtcattctct ttcttaaccg
     3121 ccgtggcttt gcgcctgcac tgctgtgcca cgactgtggc tggattgccg aatgcccacg
     3181 ttgcgatcac tactacacgc tgcatcaggc gcagcaccat ctgcgctgcc accactgtga
     3241 cagtcagcgt ccggtgccgc gccagtgccc ttcctgcggt tccacgcacc tggtccccgt
     3301 ggggctgggc accgaacagc ttgaacagac gctcgcgccg ttgttccccg gcgtgcccat
     3361 ttctcgtatc gaccgcgata ccaccagccg caaaggggcg ctggaacagc aactggcaga
     3421 agtacatcgc ggcggcgcgc ggattttgat tggtacacaa atgctggcga aaggtcacca
     3481 tttcccggat gtgacgctgg ttgcattact ggacgtggac ggcgcgctgt tttctgccga
     3541 ttttcgctcg gcagagcgtt tcgctcagct ttacacccag gtcgccggtc gtgccgggcg
     3601 tgcgggtaaa cagggcgaag tggtgctgca aacgcaccat ccggaacatc ctctgttgca
     3661 aacgttgctc tataaaggct acgacgcctt tgccgaacag gcgctggctg agcggcgaat
     3721 gatgcagcta ccgccgtgga ccagccatgt gattgtgcgt gcggaagatc ataacaatca
     3781 gcacgcgcca ttgttcctgc aacaactgcg taatctgatc ctctccagcc cactggcaga
     3841 cgagaaactg tgggttctcg gtccggttcc ggctctggca cctaaacgtg gcggtcgctg
     3901 gcgctggcag atattgttgc agcacccttc ccgcgtgcgc ttgcaacaca tcattaacgg
     3961 tacgctggcg ctcatcaata caataccgga ttcccgtaag gtgaaatggg tgctggatgt
     4021 tgatccgatt gagggttaaa ccgctcacga tgcgaggcgg atcgaaaaat tcaatattca
     4081 tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc tatctgctaa aaatgttgcc
     4141 gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca ggcgaggagt gagtgtgaaa
     4201 gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg ccctcaaggc aaaagtctct
     4261 acagcgaccg tctcccgagc attaatgaat cccgataaag tctcccaggc cacccgtaat
     4321 cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc agcctatggg gcgcaacgtc
     4381 aagcgtaatg aatcccgcac cattctggtg attgtcccgg atatctgcga tcccttcttt
     4441 agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc acggatatct ggtgctgatt
     4501 ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta tcgatttgat catcaccaag
     4561 caaattgatg gcatgttgtt gctgggttca aggctgccgt ttgatgccag cattgaggaa
     4621 cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg caccggagct ggagctgcct
     4681 acagttcata tcgacaatct gaccgccgca tttgatgcag taaattattt atatgagcaa
     4741 gggcataaac ggattggctg tatagccggt cccgaagaga tgccgctgtg tcactaccgc
     4801 ctgcaaggct atgttcaggc gctgcgtcgc tgcggcatta tggttgatcc gcaatacatc
     4861 gcccgtggcg acttcacctt cgaagccgga agcaaagcga tgcagcagct gcttgatctt
     4921 ccacaaccgc ctactgctgt cttctgccat agcgatgtga tggcgctcgg cgcactttct
     4981 caggcaaaac gccaggggct gaaagtcccg gaagaccttt ccataatcgg ttttgataac
     5041 atcgacctga cgcaattttg tgatccgccg ctgacaacca tcgcgcagcc gcgttacgaa
     5101 atcggtcggg aagctatgct gttattgctt gatcaaatgc aggggcaaca cgttggcagt
     5161 ggctctcgtt taatggactg cgaacttatc atccggggat caacacgcgc gttaccttaa
     5221 agtaaacctt taagactgcc gtgactggtc aaaggcccgc cgcttaagta acatggcggg
     5281 ctgacgaacg aataaataca gcgaaacgat agtggcacaa cgagattatg tacgccgcag
     5341 ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg aaaaagcaac gaaatctgcc
     5401 tgcggtttct cccgctatgg tcgctattgc tgccgccgtt cttgtgacct ttatcggtgg
     5461 tctgtacttc attacgcatc acaagaaaga agagtccgag acgctgcaaa gccagaaagt
     5521 gaccggaaac ggactaccac caaaaccaga agaacgctgg cgctacatta aagagctgga
     5581 aagtcgccag ccgggagtgc gtgcgcccac agaaccttct gccggtggtg aagtgaaaac
     5641 gccggagcaa ctgacaccag aacaacgtca gcttcttgaa caaatgcagg ctgatatgcg
     5701 ccagcagcca acgcagctgg ttgaagttcc gtggaatgaa cagacgccgg aacagcgtca
     5761 gcaaacgcta cagcgccaac gtcaggcgca gcaattagcg gaacagcaac gcctggctca
     5821 gcagtcgcgt acgactgaac aaagctggca gcagcagacg cgtacgtcgc aagccgcacc
     5881 ggtacaggcg caaccgcgcc agtctaaacc ggcttcttct cagcagccgt accaggatct
     5941 gctgcaaact cctgcgcaca cgactgcgca gtcgaagccg cagcaagctg cgccagttgc
     6001 tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa gacgaacgcc gctggatggt
     6061 gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg gtacgtgctc agctggcgtt
     6121 cgaaggcttt gactcgaaaa tcaccaccaa caatggctgg aatcgtgtgg tcattggccc
     6181 ggtgaaaggc aaagagaacg cagacagcac cctcaatcgg ttgaagatgg cgggtcatac
     6241 aaactgcatt cggctcgccg ccgggggttg aaaccctcaa aatccccccc atctataatt
     6301 gcattatgcc ccgtactttt gtacggggtt tgtactctgt attcgtaacc aaggggtcag
     6361 ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg tggtcatcgc tggtgatggt
     6421 caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga aaaaggtccg ccgtctgtac
     6481 aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg atgcttttac gctgttcgaa
     6541 ctgtttgaac gtaaactgga aatgcatcag ggccatctgg tcaaagccgc cgttgagctg
     6601 gcaaaagact ggcgtaccga tcgcatgctg cgcaaacttg aagcactgct ggcagtcgcg
     6661 gatgaaactg catcgcttat catcaccggt aacggtgacg tggtgcagcc agaaaacgat
     6721 cttattgcta tcggctccgg cggcccttac gcccaggctg cggcgcgcgc gctgttagaa
     6781 aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt tggatattgc aggcgacatt
     6841 tgcatctata ccaaccattt ccacaccatc gaagaattaa gctacaaagc gtaaggatct
     6901 cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac tggataagca catcatcggc
     6961 caggacaacg ccaagcgttc tgtggcgatt gctctgcgta accgctggcg tcgcatgcag
     7021 ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata tcctgatgat cggcccgacc
     7081 ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc tggcgaatgc gccgttcatc
     7141 aaagttgaag cgaccaaatt caccgaagtg ggctacgtcg gtaaggaagt ggattctatt
     7201 attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg tccaggctat cgagaaaaac
     7261 cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg acgtgctgat cccacctgct
     7321 aaaaacaact ggggacagac cgaacagcag caggaaccgt ccgctgctcg tcaggcattc
     7381 cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa tcgagatcga tcttgccgca
     7441 gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg aagagatgac cagccagctg
     7501 cagtccatgt tccagaacct gggcggccag aagcaaaaag cgcgtaagct gaaaatcaaa
     7561 gacgccatga agctgctgat tgaagaagaa gcggcgaaac tggtgaaccc ggaagagctg
     7621 aagcaagacg ctatcgacgc tgttgagcag cacgggatcg tgtttatcga cgaaatcgac
     7681 aaaatctgta agcgcggcga gtcttccggt ccggatgttt ctcgtgaagg cgttcagcgt
     7741 gacctgctgc cgctggtaga aggttgcacc gtttccacca aacacgggat ggtcaaaact
     7801 gaccacattc tgtttatcgc ttctggcgcg ttccagattg cgaaaccgtc tgacctgatc
     7861 ccggaactgc aaggtcgtct gccaatccgc gttgaactgc aggcgctgac caccagcgac
     7921 ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg tgcagtacaa agcactgatg
     7981 gcgactgaag gcgtaaatat cgagtttacc gactccggta ttaaacgcat cgcggaagcg
     8041 gcatggcagg tgaacgaatc taccgaaaac atcggtgctc gtcgtttaca cactgttctg
     8101 gagcgtttaa tggaagagat ttcctacgac gccagcgatt taagcggtca aaatatcact
     8161 attgacgcag attatgtgag caaacatctg gatgcgttgg tggcagatga agatctgagc
     8221 cgttttatcc tataatcgcg ttcaatcatt ttcatcattg tttgatgggg ctgaaaggcc
     8281 ccatttttat tggcgcgtat tatgactgaa caacaaatta gccgaactca ggcgtggctg
     8341 gaaagtttac gacctaaaac cctccccctc gcctttgctg caattatcgt cgggacagcg
     8401 ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc tgctggcact aattaccgcc
     8461 gggctattac agatcctttc taacctcgcc aatgattacg gcgatgcggt aaaaggcagc
     8521 gataaacctg accgcattgg gccgctacgc ggcatgcaaa aaggggtcat tacccagcaa
     8581 gagatgaaac gggcgctcat tattaccgtc gtgctcatct gtctctccgg gctggcactg
     8641 gttgcagtgg catgccatac gctggccgat tttgtcggtt tcctgattct tggcgggttg
     8701 tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc cttatggtta tatcggtctg
     8761 ggtgatattt ccgtactggt tttctttggc tggttgagtg tcatggggag ctggtattta
     8821 caggctcata cattgattcc ggcactgatc cttccggcga ccgcatgcgg cctgctggca
     8881 acggcagtac tgaatattaa taacctgcgt gatatcaata gcgaccgcga aaatggcaaa
     8941 aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc gttatcatgc ctgcctgctg
     9001 atgggctcgc tggtgtgtct ggcgctgttt aatctctttt cgctgcatag cctgtggggc
     9061 tggctgttcc tgctggcggc accattactg gtgaagcaag cccgttatgt gatgcgggaa
     9121 atggacccgg tggcgatgcg accaatgctg gaacgtactg tcaagggagc gttactgact
     9181 aacctgctgt ttgttttagg gatattccta agccagtggg cagcataact gacaaatatc
     9241 aattaacaat tgatgatttt gccaacagcc cacatagcgc gatatactga aaattctcgc
     9301 agcaactgaa tgttaagcct atgaaatacg atacttccga gctttgtgac atctatcaag
     9361 aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg cggacgggcg tcgtttggcg
     9421 gacaaataat cacggtaaaa tgtttcgagg acaacgggtt gctgtacgat ctgctcgaac
     9481 agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg ttctgttcgt cgcgcactgg
     9541 tcgatgctga actggcgcgt ctggcagtac aaaatgaatg ggaaggtctg gtcatttacg
     9601 gcgcggtgcg tcaggtagat gacctggaag agttggatat cggcatccag gcgatggcgg
     9661 caattccggt tggtgccgct ggcgaaggca ttggcgaaag cgatgtccgc gtcaattttg
     9721 gcggtgtcac cttcttctcc ggcgaccatc tttatgccga caataccggg attattcttt
     9781 cagaagatcc gctggatatt gaatgataag aaaggcaccg caaggtgcct tttttctgcg
     9841 ttacctgttg gcctacacag taaagaaatt acgcggaaga tgaagcgtaa tcagacctct
     9901 tccatgcgac ccagcagggc ctgcagacgt tcctgccagc cgttctgctg ttctttcaga
     9961 tggttgttct cacgctccag ctcttcgcgc tgatgctggg cattttgaac ttcctgcgac
    10021 agtgagttgt ttttttcttt cagctcttcg atttccatct gcaacagagt gatggtatca
    10081 atcgcctgct gtacttttgc ttccagtttc tcaaacactt ctaatgacat tgtcatacct
    10141 ctcctgaatt gcaaggcgtt gatggataaa aatcctcgtc ccgattaccg gtgacgcctt
    10201 aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca
    10261 gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat
    10321 ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca
    10381 tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat
    10441 tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag
    10501 catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt
    10561 atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg
    10621 ttgattttct tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttgat
    10681 cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta cctgaccgca
    10741 ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct gtttgcctgt
    10801 ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgtgct
    10861 gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca gactcatcac
    10921 attgttcgcg gcagcgttga aagtgttgat ctggctggca ctttctctac ttaccctaat
    10981 cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg
    11041 gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc tttggctccc
    11101 ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacaggtttt
    11161 gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc
    11221 aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct tttcggccct
    11281 atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg ccatttgcct
    11341 tgcgatatct gtgttgtgga agaaaaggaa accacaactc cttcagaaca aaaagcttcg
    11401 ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga
    11461 ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt
    11521 gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt tgggtagaac acgacccaat
    11581 ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag
    11641 ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga
    11701 aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga
    11761 aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct
    11821 ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg
    11881 ctctcgcgag cgtgcacgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat
    11941 ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct ctcgtaccat
    12001 gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc
    12061 gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta tacggtcaga ctaacattgg
    12121 cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct
    12181 gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt
    12241 tatgctgatg aacactggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat
    12301 cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa ggtgcggtgt ttatggcagg
    12361 cgcatccatt cagtggctgc gcgatgaaat gaagttgatt aacgacgcct acgattccga
    12421 atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg
    12481 gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc tgactcgtgg
    12541 ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc agacgcgtga
    12601 cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc gcgtggatgg
    12661 tggcgcagta gcaaacaatt tcctgatgca gttccagtcc gatattctcg gcacccgcgt
    12721 tgagcgcccg gaagtgcgcg aagtcaccgc attgggtgcg gcctatctcg caggcctggc
    12781 ggttggcttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt
    12841 ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt
    12901 taaacgcgcg atggcgtggg aagaacacga cgaataatgt aaatgccgaa tgaagcgttt
    12961 atgccgcatc cggtagtccc gaaacgtgcg ggggcaaccc cgcacacatc aataatccct
    13021 cccttcccct gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga
    13081 gaacttgcca tcgaattttc ccgcgtcacc gaatcagcgg cgctggctgg ctacaaatgg
    13141 ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg
    13201 ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca
    13261 ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct
    13321 gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg
    13381 ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat ggagaagctg
    13441 attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg
    13501 cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct
    13561 aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct
    13621 attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac
    13681 gtgctgtacg gtattggtgg cgcgccggaa ggcgtagttt ctgcggcggt gatccgcgca
    13741 ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa
    13801 gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc
    13861 ggtaaagtat tgcgcctggg cgatatggcg cgcagcgata acgtcatctt ctctgccacc
    13921 ggtattacca aaggcgatct gctggaaggc attagccgca aaggcaatat cgcgactacc
    13981 gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc catccactat
    14041 ctggatcgca aagacccgga aatgcaggtg cacatcctct gattgatttg atcgattgag
    14101 ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa
    14161 tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac
    14221 tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt taccgccggg
    14281 caatttacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc ctactcctat
    14341 gtaaactcgc ccgataatcc cgatctggag ttttacctgg tcaccgtccc cgatggcaaa
    14401 ttaagcccac gactggcggc actgaaacca ggcgatgaag tgcaggtggt tagcgaagcg
    14461 gcaggattct ttgtgctcga tgaagtgccg cactgcgaaa cgctatggat gctggcaacc
    14521 ggtacagcga ttggccctta tttatcgatt ctgcaactag gtaaagattt agatcgcttc
    14581 aaaaatctgg tcctggtgca cgccgcacgt tatgccgccg acttaagcta tttgccactg
    14641 atgcaggaac tggaaaaacg ctacgaagga aaactgcgca ttcagacggt ggtcagtcgg
    14701 gaaacggcag cggggtcgct caccggacgg ataccggcat taattgaaag tggggaactg
    14761 gaaagcacga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat
    14821 ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat gacgaaacat
    14881 ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga
    14941 taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga tatgccggca
    15001 tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac tgctcttttt
    15061 gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg
    15121 gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt
    15181 gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat
    15241 caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca
    15301 ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt
    15361 ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat
    15421 catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc cctccagatc
    15481 cttaatctta taggccacgc tggccccttc atctgcagag gatgtagcgg cggcaagctc
    15541 tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt
    15601 actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact
    15661 gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac
    15721 gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc
    15781 attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggc attgtcattt
    15841 tcatcgtgga aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat
    15901 tacttgattt attgtcggct ttatacttca catcctgggt atctttacca tatttatttt
    15961 cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga atcaacgtcg
    16021 ggacaaaacg ccccaccgcc cattgccaga cacccggtaa aatcgcccag ttacccgcca
    16081 gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca
    16141 agcgcttaac ggttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcgg
    16201 tctgaatatc gagtaaattt ttacccgcca gtgagaaaag caccagcatg cctgcgaacc
    16261 acaggcctat ccaaatccag aaatcacggt gtccaatacg ccctttaaat gagaataacc
    16321 attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tccaggattc tacccttttg
    16381 acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga
    16441 agccagggtg tacgctgttt tttctcttat gttctgcatt aaccgttaca acagcggcgc
    16501 atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt
    16561 tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac
    16621 tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcaa
    16681 gtaaaattaa tgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca
    16741 aaagcattca aatgacctgg ctacccatcc aggggccaga gcaaaaagcc gcgaaagcga
    16801 aagctcagga atacatggca gcggtgatcc gcacactcac cccattaatg accaaaacac
    16861 aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg
    16921 agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg
    16981 ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa
    17041 aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg
    17101 gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt
    17161 gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct
    17221 gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta
    17281 catcgatatg gcgaagcgcg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt
    17341 ggacctgaac ctgtccggcg cattcaccgg tgaaacttct gccgctatgc tgaaagacat
    17401 cggcgctcag tacatcatca tcggccactc tgaacgtcgt acttaccaca aagagtctga
    17461 cgaactgatc gcgaaaaaat tcgcggtgct gaaagagcag ggcctgactc cggttctgtg
    17521 catcggtgaa accgaagctg aaaacgaagc gggcaaaact gaagaagttt gcgcacgtca
    17581 gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta
    17641 cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt
    17701 tcacaaattc atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat
    17761 cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga
    17821 tatcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg cagtgatcgt
    17881 taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg
    17941 cacttacttt ccttaactct tcgccttaac gcaaaatctc acactgatga tcctgaattt
    18001 cctcggctga agcacggtta agcgtcagta gatttcgttg tgtcgccagc aatacaaatg
    18061 agttatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag
    18121 gtacttcttc tgccagcatt ataaatgggc tgcgttgtac cagttcgctt tccgttaccc
    18181 gacgcgccag gtattcatgc ccgcgcaaac cacctggcag tggcaaccag cggctgctga
    18241 tgttcgccag attgttatcc agttgttcgc gcacatcagg acgaatacaa gagatatgaa
    18301 tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccaaagaa accgcgcgat
    18361 cgggaaccgg ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa
    18421 agaagttcgg cgttgaagga tcggtcaaca aaggactttc agtaccgtta atacgatacg
    18481 ttggcatcaa cagatattgc agtggaccat taagatcttt taaaaccacg tatccggcat
    18541 tgggtttgac ttccgcacat ggcgaaggat tttgattttg ctgctgattg ggcaaacatt
    18601 cctcaaggac aatcttacgt aatgtatccg actcttcacc ggttaatttc cagtaaccaa
    18661 taccggcagc cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca
    18721 tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    18781 aacccggtcg cgagaaacca ccgggtttga taattatcct ggggaaatca gcgtttgctg
    18841 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    18901 aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgcc
    18961 acctcagcgt cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag
    19021 tagagatatt tcaggtaggc ttccgccacc tctttagtac cttttttctc gaccacttta
    19081 tcgaccaccg acacggttgg ttccgcgagg atagactcac tcggcgtgac gatttcgaat
    19141 ttatctttcc ccagttcatt cgctgccaac agagcttcgt tttcccaggc aatcagtaca
    19201 tcgccaattc cgcgctcgac aaaagtgtta gtggagccgc gcgcgccaga atccagaact
    19261 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    19321 tgatgcagcg cgtagcccca ggctgccagg taattccagc gcgcgccacc agagcttttc
    19381 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    19441 ttcggatttc ccttacgtac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    19501 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaattgc gtccacgtca
    19561 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    19621 ttacctgagc caccgtgtga ctgacgaatc accacgttat caccagtttg ctgtttccag
    19681 tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    19741 ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct
    19801 acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    19861 agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt
    19921 tgggggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    19981 atgccggatg cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat tcaataaatt
    20041 gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg
    20101 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    20161 cagccagtcg cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    20221 aaccaactgt tcgttctgga tacctacgca acgaccgccg taacctgcca gcagcagatc
    20281 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    20341 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    20401 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt
    20461 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    20521 cggaaccaca acgaattcac agccaccggc aatggccgca gccaacgtca gatcgccaca
    20581 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    20641 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    20701 gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt
    20761 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc
    20821 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    20881 acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagacacgc tgtaacggtc
    20941 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatgc ccattacttc
    21001 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    21061 atcaccgccg cttgtcaaca caccgatttt cttaatcatg actacctctg aactttggaa
    21121 tgcaaaataa aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    21181 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    21241 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    21301 accctccctg ggtacgacgg aacaggggtc ctgatggata attacatccg atcccggaaa
    21361 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    21421 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    21481 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    21541 ttcctcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    21601 cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca
    21661 gccgtaccag gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg
    21721 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cgccgcacca cccaacgctg
    21781 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    21841 tggatctgtc atcggtgttg gagatatcag atgttgaata cccgtcaaaa acaggaatag
    21901 tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactctg ctttaccgtg
    21961 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    22021 caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    22081 ggtataccac catgcaaaaa ttttaatcag caatagcaac gaagccatcg ccgtcgcagc
    22141 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    22201 ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    22261 gataaaaaaa acccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    22321 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    22381 tttgccattg cgtcacgtcg cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa
    22441 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    22501 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    22561 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    22621 gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    22681 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    22741 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    22801 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    22861 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    22921 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    22981 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    23041 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    23101 ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa aaatggtatc
    23161 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    23221 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    23281 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg
    23341 agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttagtg
    23401 ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggtact
    23461 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat
    23521 ttaagccagg aagtgctggg caaacgcctg acgcctttcg accgcgctat cgatatgcac
    23581 atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc
    23641 ttgcgtggtc gcggctacct gatggtttct gcttcatgat aggcagctta accgcgcgca
    23701 tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca
    23761 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag gggctgatga
    23821 ttgagcagca tgttgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    23881 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga
    23941 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    24001 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    24061 tcggtccgtt ctccgtacgt gatggcgaag ataattacca actttatctg attcgtccgg
    24121 ccagcagttc tcaatccgat ttcattaact tactgtttga ccgcccgcta ttactgctga
    24181 ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    24241 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc
    24301 cggaactgga agcggggcca caggaattcc ttgccgcagg tgccagtttt aaccagatgg
    24361 tcaccgcgct ggagcgcatg atgacctctc agcagcgtct gctttctgat atctctcacg
    24421 agctgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    24481 gtgaaagcaa ggaactggag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    24541 acgatctgtt ggtgatgtcc cgtaatcagc aaaaaaacgc gctggttagc gaaaccatca
    24601 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcagatgg
    24661 gcaagtcgtt gacggttaac ttcccgcctg ggccgtggcc gctgtacggc aatccgaacg
    24721 ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga
    24781 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    24841 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg accgatgaag
    24901 cacgcgatcg tgaatctggc ggtacaggat tggggctggc gattgttgaa accgccattc
    24961 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    25021 tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt
    25081 acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc
    25141 ctacactccg ggggttattt tccccacaac cggcgggaaa aatcttcgat cttgccgctc
    25201 agtcggcgct tttgcatcgt ccgcgtccag cttttcgata acccggcggc ggagagtaaa
    25261 cggtgatatt gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg
    25321 gtcacatcgc tgacacggga aaccaactcc agcggcgcgt ccgcagacac tttccctggc
    25381 gctatcacgc tatacaacca ccccacttta cccgtgtttt gcatcaactg cgctatatcg
    25441 ctgatatcaa aatggtaatt gagtttatag caaggcgagc gcggctggct gacctgaatt
    25501 aatgcctctc cccagcggaa aatatcgccc atataaacat tactttccgt gagaccgtcg
    25561 gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc
    25621 cgcgcccagt agagatagtg ctcacgagga taatgacaca gcgctctgtc tggcccgccg
    25681 tgaacctttt tctccgcctg ctcgtcacct tccagcccca gctccgtcag catcaactca
    25741 ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg
    25801 cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt
    25861 agaccatatt tattttatgt caggcgtgct gttttcgcgg taagtatcca ggcaggaact
    25921 caatagcaaa ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta
    25981 tttcaccgta cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat
    26041 agaggtaaga attggcacca gaatcgaagt cacaatgacc gccgtcgcta ccagcgaagt
    26101 tgctgccgga gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc
    26161 taccgcggcc cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat
    26221 caatttatcg gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag
    26281 gatccccagc aaaccagtct gggcaatcac agtcaaatca atggtattgc ccagcgcgaa
    26341 ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa aattctcgta attcagggtc
    26401 aaggttcccc agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg
    26461 ttcaaacgag gcaatccctg cagtgcccag aataatcatc gtcatgagcg gcccggactc
    26521 caacgacatc aacacaaatg ccccagcttc ttcttttgtg ccgtactgct gcatgatgga
    26581 agcgtaaagt ccgccattgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc
    26641 ggcaaagaat ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac
    26701 cacccacgcg acggcaattt ttgttaccac cagcgtaccg gatttacgca ataccgttcc
    26761 cgtcgcgctt aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac
    26821 cgtaccggta atcatcccgt tggtgaatga tccaaaatat ttccccgccc ccggcgagaa
    26881 ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc atcccccccg ggattttctc
    26941 aatcgagcgt tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt
    27001 cattcctcaa tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata
    27061 aaagtgatca aaaccacatc aattgaaacg ctgttttatt tttatcggat cattgttttt
    27121 attttgaaat tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga
    27181 tatgcggcct atacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcaaaac
    27241 gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc tttaatgtag tccggacggc
    27301 ggttctggaa tttcaggtag taagcatgtt cccacacatc caggcccata atcgggaagc
    27361 cggaagcgcc agaaatagct tcacccatca gcggagaatc ctggttagca gtagaaacca
    27421 ccgccagttt atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg
    27481 ccgctttttc aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg
    27541 ctttcaggtc accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt
    27601 tagcgtgacc gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca
    27661 gtttggtgat cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg
    27721 cgttggcgtt gttgacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct
    27781 tatcgaagtg cggttccagg gcatcgtaag catacggcag ggatggcagg gtatagctca
    27841 tattcatctc cagtattgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat
    27901 tatagttaat taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg
    27961 gttttgcagg aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca
    28021 gtaaatcgac aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca
    28081 tctcgtcgga gatgtgacgc gacgaaaaat gatgaggata agaagatgag taacgcgatt
    28141 acgatgggga tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg
    28201 ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt cagtcggtgg gattgtttcg
    28261 tggattattc tgccgtgggc catcagcgcc ctgttactac cgaatttctg ggcgtattac
    28321 agctcgttta gtctctctac gctactgcct gtttttctgt tcggcgctat gtgggggatc
    28381 ggtaatatca actacggcct gaccatgcgt tatctcggca tgtcgatggg aattggcatc
    28441 gccattggca ttacgttgat tgtcggtacg ctgatgacgc caattatcaa cggcaatttc
    28501 gatgtgttga ttagcaccga aggcggacgc atgacgttgc tcggcgttct ggtggcgctg
    28561 attggcgtag ggattgtaac tcgcgccggg cagttgaaag agcgcaagat gggcattaaa
    28621 gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct
    28681 gccgggatgt cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgccgcactt
    28741 ggcgtcgatc cactgtatgt cgctctgcca agctatgttg tcatcatggg cggcggcgcg
    28801 atcattaacc tcggcttctg ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa
    28861 gccgacttct cgctggcaaa atcgctgatc attcacaatg tgttactctc gacactgggc
    28921 gggttgatgt ggtatctgca attctttttc tatgcctggg gccacgcccg cattccggcg
    28981 cagtatgact acatcagttg gatgctgcat atgagtttct atgtattgtg cggcggtatc
    29041 gtcgggctgg tgctgaaaga gtggaacaat gcaggacgcc gtccggtaac ggtgttgagc
    29101 ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg gcatcggcat ggcgaattaa
    29161 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
    29221 accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac
    29281 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgtcg cagatattga
    29341 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
    29401 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta
    29461 atcagcttat ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga
    29521 tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac
    29581 ggcacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc
    29641 cccatgctac ctaagcgcca gtgtggttgc cctgcgctgg cgttaaatcc cggaatcgcc
    29701 ccctgccagt caagattcag cttcagacgc tccgggcaat aaataatatt ctgcaaaacc
    29761 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca
    29821 cgggtaatgc gataagggcg atcgttgagt acatgcaggc cattaccgcg ccagacaatc
    29881 accagttcac aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc
    29941 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
    30001 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
    30061 acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg
    30121 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca
    30181 gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc gggctttcat
    30241 cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg
    30301 atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
    30361 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgcgatgcgc tgttttccag
    30421 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg
    30481 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
    30541 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
    30601 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    30661 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    30721 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    30781 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    30841 ctgcgggagc cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact
    30901 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    30961 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
    31021 atacagcgtg aattttcagg aaatgcggtg agcatcacat caccacaatt cagcaaattg
    31081 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
    31141 agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca
    31201 ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc
    31261 tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca
    31321 atgggctgca tagtcagaac ggctatgtca cctgggatgt ggatagcctt gaaagtgcca
    31381 ttcgccttgg attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg
    31441 atacctgggg cgtggacttt gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg
    31501 ttgcttatcg cgatagccgc accaatggcc taatggcgca ggcacaacaa caactcggca
    31561 aacgcgatat ttatcaacgt agcggcatcc agtttctgcc cttcaatacg ctttatcagt
    31621 tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga
    31681 tgccggatta cttcagttat cgcctgaccg gcaagatgaa ctgggaatat accaacgcca
    31741 cgaccacgca actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga
    31801 gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc ataggtcact
    31861 ggatttgccc gcagggtaat gagattccag tggtcgccgt tgccagccat gataccgcca
    31921 gcgcggttat cgcctcgccg ttaaacggct cacgtgctgc ttatctctct tctggcacct
    31981 ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggca ctggcagcca
    32041 acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct
    32101 tatggctgct tcagcgagtg cttcaggagc agcaaatcaa cgatcttccg gcgcttatct
    32161 ccgcgacaca ggcacttccg gcttgccgct tcattatcaa tcccaatgac gatcgcttta
    32221 ttaatcctga gacgatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga
    32281 tcccggaaag tgatgctgaa ctggcgcgct gcattttcga cagtctggcg ctgctgtatg
    32341 ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga tttctcgcaa ctgtatattg
    32401 tcggcggagg ctgccagaac acgctgctca accagctatg cgccgatgcc tgcggtattc
    32461 gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa tatcggcatc cagttaatga
    32521 cgctggatga actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc
    32581 tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg cggattcact
    32641 ctacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct
    32701 ggcgaaacag cgtttcgcgg cagtagggat tgatgtcgag gaggcgctgc gccaacttga
    32761 tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc
    32821 ggaaggttcg ctgaccggtg ggattcaggc tactggtaat tatccgggca aagcgcgtaa
    32881 tgccagtgag ctacgagccg atctggaaca agctatgcgg ctgattccgg ggccgaaacg
    32941 gcttaattta catgccatct atctggaatc agacacgcct gtctcgcgcg atcagatcaa
    33001 accagagcac ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg gtctggattt
    33061 taacccctcc tgtttttcgc atccgctaag cgccgatggc tttacgcttt cccatgccga
    33121 cgacagcatt cgccagttct ggattgatca ctgcaaagct agccgtcgcg tttcggccta
    33181 ttttggcgag caactcggca caccatcggt gatgaacatc tggatcccgg atggtatgaa
    33241 agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt
    33301 gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc gttgagagca aattgtttgg
    33361 cattggcgcg gagagctaca cggttggctc caatgagttt tacctggggt atgccaccag
    33421 ccgccagacg gcgctgtgcc tggacgccgg gcatttccac ccgactgaag tgatttccga
    33481 caagatttcc gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt
    33541 tcgctgggac agcgatcacg tagtgctgct ggatgatgaa acccaggcaa ttgccagtga
    33601 gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc cttgacttct tcgatgcctc
    33661 tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg
    33721 tgcgttgctg gaacctaccg ctgagctgcg caagctggaa gcggcgggcg attacactgc
    33781 gcgtctggca ctgctggaag agcagaaatc gttgccgtgg caggcggtct gggaaatgta
    33841 ttgccaacgt cacgatacgc cagcaggtag cgaatggctg gagagcgtgc gggcttatga
    33901 gaaagaaatt ttgagtcgcc gcgggtaaac actgccggat gcggcgcgag cgccttatcc
    33961 ggcctacggg tcgtgcatcc gacaacaccg aatttacagg aacacagaac atgcaaaaca
    34021 ttactcagtc ctggtttgtc cagggaatga tcaaagccac caccgacgcc tggctgaaag
    34081 gctgggatga gcgcaacggc ggcaacctga cgctacgcct ggatgacgcc gatatcgcac
    34141 catatcacga caatttccac caacaaccgc gctatatccc gctcagccag cccatgcctt
    34201 tactggcaaa tacaccgttt attgtcaccg gctcgggcaa attcttccgt aacgtccagc
    34261 ttgatcctgc ggctaactta ggcatcgtaa aagtcgacag cgacggcgcg ggctaccaca
    34321 ttctttgggg gttaaccaac gaagccgtcc ccacttccga acttccggct cacttccttt
    34381 cccactgcga gcgcattaaa gccaccaacg gcaaagatcg ggtgatcatg cactgccacg
    34441 ccaccaacct gatcgccctc acctatgtac ttgaaaacga caccgcggtc ttcactcgcc
    34501 aactgtggga aggcagcacc gagtgtctgg tggtattccc ggatggcgtt ggcattttgc
    34561 cgtggatggt gcccggcacg gacgaaatcg gccaggcgac cgcacaagag atgcaaaaac
    34621 attcgctggt gttgtggccc ttccacggcg tcttcggcag cggaccgacg ctggatgaaa
    34681 ccttcggttt aatcgacacc gcagaaaaat cagcacaagt attagtgaag gtttattcga
    34741 tgggcggcat gaaacagacc atcagccgtg aagagttgat agcgctcggc aagcgtttcg
    34801 gcgttacgcc actcgccagt gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct
    34861 ttgtcatgca ggtaaacccc gacgcccacg aagagtatca gcgtcggcat aatcccatct
    34921 ggccagaact ggaagcagtg ctgaaatctc acggtgcgca taactacgcc atctatctcg
    34981 acaaagcgcg taatctgctg tttgccatgg tagagattga atctgaagaa cgctggaatg
    35041 cggttgccag cactgatgtt tgccaacgtt ggtggaaata tatgaccgat gttatgcccg
    35101 ctaacccgga taacagcccg gtgagtagcg agctgcaaga agtgttttac ctgccgtaat
    35161 tgcccaatgt gccagagatg cctccggcac aatattttaa atttcgattt gaaaaattaa
    35221 acatattaca tctcccggta agttatattt ccctgataca ttgtgagtaa atcacaaaaa
    35281 taatgaataa cccattaatg attcatgtgg tttatttaaa taaccattat gtgcattact
    35341 ccgcaaatct gacctttcac tctgtctcaa attgtcattg tttaccgaca tatcgcctct
    35401 catcacttaa tcgctccacc attacaattg attaaaatat attttatgga gtccgattaa
    35461 tggcagctct tactgcaagc tgtattgacc tgaatattca gggcaatggc gcttattccg
    35521 ttctgaa
//
LOCUS       DACAPB010000026        34301 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.026, whole genome shotgun sequence.
ACCESSION   DACAPB010000026 DACAPB010000000
VERSION     DACAPB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.026"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..83)
                     /locus_tag="IT904_001141"
     CDS             complement(<1..83)
                     /locus_tag="IT904_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAP3190955.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVR"
     gene            complement(286..687)
                     /gene="nikR"
                     /locus_tag="IT904_001142"
     CDS             complement(286..687)
                     /gene="nikR"
                     /locus_tag="IT904_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAP3190956.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(693..1499)
                     /gene="nikE"
                     /locus_tag="IT904_001143"
     CDS             complement(693..1499)
                     /gene="nikE"
                     /locus_tag="IT904_001143"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAP3190957.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(1496..2260)
                     /gene="nikD"
                     /locus_tag="IT904_001144"
     CDS             complement(1496..2260)
                     /gene="nikD"
                     /locus_tag="IT904_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAP3190958.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAVALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2260..3093)
                     /gene="nikC"
                     /locus_tag="IT904_001145"
     CDS             complement(2260..3093)
                     /gene="nikC"
                     /locus_tag="IT904_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAP3190959.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3090..4034)
                     /gene="nikB"
                     /locus_tag="IT904_001146"
     CDS             complement(3090..4034)
                     /gene="nikB"
                     /locus_tag="IT904_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAP3190960.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4034..5608)
                     /gene="nikA"
                     /locus_tag="IT904_001147"
     CDS             complement(4034..5608)
                     /gene="nikA"
                     /locus_tag="IT904_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAP3190961.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5719..6306)
                     /gene="acpT"
                     /locus_tag="IT904_001148"
     CDS             complement(5719..6306)
                     /gene="acpT"
                     /locus_tag="IT904_001148"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAP3190962.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHS
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6361..7410)
                     /locus_tag="IT904_001149"
     CDS             complement(6361..7410)
                     /locus_tag="IT904_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP3190963.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7542..8759
                     /locus_tag="IT904_001150"
                     /pseudo
     CDS             7542..8759
                     /locus_tag="IT904_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(8763..9320)
                     /gene="dcrB"
                     /locus_tag="IT904_001151"
     CDS             complement(8763..9320)
                     /gene="dcrB"
                     /locus_tag="IT904_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAP3190964.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9393..10058)
                     /locus_tag="IT904_001152"
     CDS             complement(9393..10058)
                     /locus_tag="IT904_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAP3190965.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10279..10524
                     /gene="tusA"
                     /locus_tag="IT904_001153"
     CDS             10279..10524
                     /gene="tusA"
                     /locus_tag="IT904_001153"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAP3190966.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLSYRYLIRKGG"
     gene            complement(10626..12824)
                     /gene="zntA"
                     /locus_tag="IT904_001154"
     CDS             complement(10626..12824)
                     /gene="zntA"
                     /locus_tag="IT904_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAP3190967.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVERLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12898..13524)
                     /locus_tag="IT904_001155"
     CDS             complement(12898..13524)
                     /locus_tag="IT904_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAP3190968.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLVGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13665..14024
                     /locus_tag="IT904_001156"
     CDS             13665..14024
                     /locus_tag="IT904_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAP3190969.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14027..14296)
                     /locus_tag="IT904_001157"
     CDS             complement(14027..14296)
                     /locus_tag="IT904_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAP3190970.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14286..14882)
                     /gene="rsmD"
                     /locus_tag="IT904_001158"
     CDS             complement(14286..14882)
                     /gene="rsmD"
                     /locus_tag="IT904_001158"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAP3190971.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAEATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15032..16528
                     /gene="ftsY"
                     /locus_tag="IT904_001159"
     CDS             15032..16528
                     /gene="ftsY"
                     /locus_tag="IT904_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP3190972.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAGVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16531..17199
                     /gene="ftsE"
                     /locus_tag="IT904_001160"
     CDS             16531..17199
                     /gene="ftsE"
                     /locus_tag="IT904_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP3190973.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDLNLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17192..18250
                     /gene="ftsX"
                     /locus_tag="IT904_001161"
     CDS             17192..18250
                     /gene="ftsX"
                     /locus_tag="IT904_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAP3190974.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18495..19349
                     /gene="rpoH"
                     /locus_tag="IT904_001162"
     CDS             18495..19349
                     /gene="rpoH"
                     /locus_tag="IT904_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAP3190975.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19620..20723
                     /locus_tag="IT904_001163"
     CDS             19620..20723
                     /locus_tag="IT904_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP3190976.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARSYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20922..21305)
                     /gene="panM"
                     /locus_tag="IT904_001164"
     CDS             complement(20922..21305)
                     /gene="panM"
                     /locus_tag="IT904_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAP3190977.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            21729..22838
                     /gene="livk"
                     /locus_tag="IT904_001165"
     CDS             21729..22838
                     /gene="livk"
                     /locus_tag="IT904_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAP3190978.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            22886..23812
                     /gene="livH"
                     /locus_tag="IT904_001166"
     CDS             22886..23812
                     /gene="livH"
                     /locus_tag="IT904_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAP3190979.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23809..25086
                     /locus_tag="IT904_001167"
     CDS             23809..25086
                     /locus_tag="IT904_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAP3190980.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25083..25850
                     /gene="livG"
                     /locus_tag="IT904_001168"
     CDS             25083..25850
                     /gene="livG"
                     /locus_tag="IT904_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAP3190981.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25852..26565
                     /gene="livF"
                     /locus_tag="IT904_001169"
     CDS             25852..26565
                     /gene="livF"
                     /locus_tag="IT904_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAP3190982.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQLQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDVLLANEAVRSAYLGG"
     gene            26962..28278
                     /gene="ugpB"
                     /locus_tag="IT904_001170"
     CDS             26962..28278
                     /gene="ugpB"
                     /locus_tag="IT904_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAP3190983.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSLPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28376..29263
                     /gene="ugpA"
                     /locus_tag="IT904_001171"
     CDS             28376..29263
                     /gene="ugpA"
                     /locus_tag="IT904_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAP3190984.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29260..30105
                     /gene="ugpE"
                     /locus_tag="IT904_001172"
     CDS             29260..30105
                     /gene="ugpE"
                     /locus_tag="IT904_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAP3190985.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30107..31177
                     /locus_tag="IT904_001173"
     CDS             30107..31177
                     /locus_tag="IT904_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HAP3190986.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31174..31917
                     /gene="ugpQ"
                     /locus_tag="IT904_001174"
     CDS             31174..31917
                     /gene="ugpQ"
                     /locus_tag="IT904_001174"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP3190987.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(31904..32344)
                     /locus_tag="IT904_001175"
     CDS             complement(31904..32344)
                     /locus_tag="IT904_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAP3190988.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32464..34206
                     /gene="ggt"
                     /locus_tag="IT904_001176"
     CDS             32464..34206
                     /gene="ggt"
                     /locus_tag="IT904_001176"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAP3190989.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
BASE COUNT         7931 a         8939 c         9626 g         7805 t
ORIGIN      
        1 atgcgcaccc cggctgaacc ctgaacaatg ctaccgccat actgcgtcat gtcaccctga
       61 cgcgccgccg gttttccgct catcgtaaaa tccttttcta ttattttaat gacagtatct
      121 tcatacgaaa aagcgaagaa aaacttgtgg tcacaatgaa ttgaagattt ttaggaatat
      181 atttagaacg ttacaaaaga gaaggttcgg atagggtatt tagagaggaa tcaggtgtgc
      241 tagtggccct ttgttgatcg ttcaccaaag gacccaataa tagactcaat cttccttcgg
      301 caagcactgc aaatgcccgt gccgcacgcc gcgctgggcg ataacgtcat cggcaaaatg
      361 ctgcacgtca cccatgtcac ctttcaacac ggcgatttcc agacagtcgt cgtggttgat
      421 gtgcacatgc agcgtggcga cggagaggtc gtggtgatga tgctgggtgg aaacaatacg
      481 gctggctaag tcgcgttttt cgtgttcata cacatacgac agcaccgcga aaccttgtgt
      541 gccgtgctgc tgggtagctt cctgcgccag ggcgctacgc agaatatcgc ggatagcttc
      601 ggaacggttg ttataaccac gacgctggct caggctgtcc agcgtctcca gtaaatcgtc
      661 atcaagcgtg atggtgactc gttgcatttg cgttaaacct tttctgaggt gcgacggcgc
      721 acggggaatg cgggtaatac cgcgttttgt agcacacgtc cggcgtcaga ggaaaaggtt
      781 aatttatctc ccaccgcctg ggtttcgacg atttgtccgt tgtccatcac catcactcgc
      841 tggcaaaagc gttccaccag gcgtaagtcg tgggtgatga acaggcaggc agtgccaaac
      901 tgttgttgta gctttttcag caggcgaatg acgcccgcct gtaacacgag atcaaggtta
      961 gaaacggctt catccagaat cagtagtttc ggttcgaccg ccagtgcgcg agccaggcag
     1021 acgcgctgga gctggccgcc gcttaactgc ggtgggcgtt tgtcgagaac gctgtcatcg
     1081 agatcgaccg ccttcagcat ttcgctggcg cgcgccagtt gttcggattt tttcagtgat
     1141 agcaggtggc gcatcggttc acgcaggatc tcgcgcacgg ttttgcgcgg attcacggcg
     1201 ctgatggagt cctgaaatac catctgaata tcgcggcgga acgctttacg ctgggcgcga
     1261 ttgagtttcg ccagcggttc gccgcgccag ctaatattcc cttgtgaggg agactctaag
     1321 cctaccagca gccgcgcgag ggtgcttttt ccgcagccgc tgcgtcccag cagggcgacg
     1381 gtttcgccgc ttttcagggt cagggaaacg ttattcagca ccgcctgatg ttgatgtttt
     1441 ccgctaaatc caccgtgggc atagtgatgg gaaaggccgc agacgttaag taaggtcatg
     1501 atgccagctc cataccgtag agggcgagat gagcggaaac caggctgcgc gtcaccgtat
     1561 gtttgggggc gttaaacagc gtttctacat cgccctgttc gacaatttta ccttgtgaca
     1621 tcaccgccac gtcatccgcc agacgcgcca caacgcccat atcatgggtg accagcagca
     1681 ttcccggcgc ttgtttttgc ataatgcttt ccagcagatc gaggatgcgc gcctgtgcta
     1741 ccacgtcgag gtcggtggtc ggttcatcgg cgatgataaa cggtgattca cacagcaccg
     1801 ccatcgcaat catcatgcgc tgcaacatgc cgccgctcat ctcgaacggg tacagcttca
     1861 gcacgcgcgc ggcgttttcc agccccaccg cttctatggc agcggtaagc gtagcgtcat
     1921 cggcgggttt ccctaacgcc aggcaggttt cccgcgcgtg ggtgtgcatg gtgtgcagtg
     1981 gattaaaggc gctgcgcggg ttctgcatga tggtggcaat tttgatgccg cgcagggcgc
     2041 aaggcgaaac cggtttgcca tcggctaaaa tttcccccgc cgtctggcga acgcctgcgg
     2101 gcagaattcc cagcgccacg gcgcaggtta atgatttccc gctgccgcta ccgccgacta
     2161 acgccagcac gcgcccgcgt tgcagggtta acgatacacc gtgtaccagc ggctgcgcgg
     2221 cctgtagcgc gatattacgt agttcaatct gttgcggcat tagtgtgcgt gctccgtcac
     2281 cagatgaggg tccagatgat cgcgcagtgc gtcacccacc aggttaaagg ccatcacgct
     2341 gataaacagc gccagccccg gccagaacat ttgcagcggc tgggtccaga tatactggcg
     2401 cgcgtcgtta atcatcacgc cccattcggc ggtcggcgcg gtcacaccga ggccaaggaa
     2461 agacatcccc gcgacgtgca gcatcatatg gccgatatcc agcgttgcca gcaccagcag
     2521 cgaagggatc accgcgcctg ccagatgatc gacaaacacc cgcacatggc ccgcaccgga
     2581 aagccgtgac gccagcacaa actcgcgttg gcgtagtgaa atcaccaggc tgcgcaccat
     2641 gcgtgcatac cacgcccagt gcgacagggc gatggcgata attacgttgg tcagcccggt
     2701 gccgagcacg ccaaccataa agaacgacag aatcgaggtc gggaaggtca taaacatatc
     2761 ggcgacgcgc atggtggcct gatcaacgcg cccgccaatc aacccggcgc tgccgccaat
     2821 aaccagccct aatgtcagca ccagcagcag gcaggccatt accgaaccga gcgacacgcg
     2881 ggtcgctgcc atcagccgcg agaaaatatc gcgacctaag tgatcggtgc ccagccagtg
     2941 ctgcgcatcc ggcgaaagca ggcgcgacgg caaatcaatc gcctgggggt catacggcag
     3001 ccaccactgg ctggtgagcg caatcagcgc cagcagggcg ataatgatca gcgccaggcg
     3061 taccgaccag cgggaagaga ggaaaaagtt cacgcgtgcg ctccttcatg acgacgaatg
     3121 cgcgggtcca gcgcggcgtt gagcaaatcg acaatcaaat tacagaccac aaaaaccacc
     3181 accatcatca gcgtaaagca ctggatcacc ggatagtcac ggttaaaaat cgccgacacc
     3241 gcatagcgcc cgacgcccgg ccaggcaaag atgttttcga taatcatcgt cccgccaatc
     3301 agttcgccga tatgcatccc cacggcggtg atcatcggca gcgaggcatt gcgcaggatg
     3361 tggcgacgtt cggtctgttt gtcgttcagg ccgcgcagac gcgcccaggt gacgtgacgc
     3421 tgaccggcga cgtccagcat actggcgcgc agtaaacgcg cgttaatcgc cagcgacata
     3481 aaggcaatgg aaaccgcagg caaaatgatg tgctgccagc cgccgtaacc catcgcgggt
     3541 agccattgca gatacaccga aaacgccatc accagtaaaa acgccagcca gaagttaggc
     3601 atcgacacgc caagaaacgc gatgaaacgt acggcgaaat ccggcagacg gtcgcgatgg
     3661 cgcgccgccc agataccgag cggtacggaa gtgagcagaa ttaataccag cgccgcacct
     3721 gcaagttcca gcgtggcggg caggaagttc agcatatcgt ccagtaccgg gcgttggctg
     3781 gcgaatgaga taccaaagtc aagatgcagc gccttccaca accaggtgcc gtactggacg
     3841 tacagcggct gatccagtcc cagcatggtg cgggtagagg ccagcatctc cggcgtcggc
     3901 ggcaggttag acagacgcaa ataatcgagc gccgggtcgc cggtaccgag gcgcagcatc
     3961 agaaaaatga tcaccgaggc ggcgagcacc atcgggatca gcagcagaaa gcggcgtaat
     4021 acgtaacgca acattaaggt ttcaccggtt taatctgttc gaacggaatt tcggtggcga
     4081 tcggcgcgta ggggatgtta cccagctccg gttttgatac caccatcatt gagatgtaac
     4141 tgataggcag ataaaccgcc tcgtcatgca gacgggtcag aatgtcgcga tacagcgcct
     4201 gacgttgcgt ttcgtcatgg gtcgccagca cttcgccgat ctctttatca atcagcggtt
     4261 tgtcggctaa tccttgctgt gcctggaagt cagcgtgtga cggtacgcgc attgaactga
     4321 ggaaggcgtg tggatcatat ggcgcgcccc aggtgcggtg gaaaatcatg ccaaaacgac
     4381 cgtcgcgctg acgagcatag atactgctct cttcttcgcc aatcagcgag acatctgcgc
     4441 caatctggcg catatcagcc tgaatgattt ccgccatcga tttgcttaac gcatcggtgc
     4501 cgatgaacga aagttcaatg cgcagcggct gaccattttt ctcgcggatg tctttgcccg
     4561 caggcagcgt ccaaccggct ttttccagca acgctttcgc tttttgcgga tcgtactggc
     4621 gcggtttcag gccgaggttg gcgtagggca cagaaggggc aaacagggtg tcggcgacct
     4681 gctgggtgcc atacaacgcg ttatcaatca gcgatttttt gtttaccgcg taattaagag
     4741 cttcacgtac tgccagctcg ttggtggggg ctttggcggt attgagcgcc agcatcacgg
     4801 tttcgatcgg ctgtgacagt tgggtgtggt aagccggatt ctggctaaag cgggcgaagg
     4861 tatcgagcgg taataaccct tcgtttccgt acagcaggtc gatatcgcca gtttcaaacg
     4921 ccaccgcgcg ggtagtcggg tccgggatga cgttaaaggt gatcttttta atcgctggct
     4981 tttcgcccca gtagttttcg ttacggacga agacatcgta ctgattcagt ttcgattcct
     5041 gcaaaatcca cggtccggtg ccaatcggcg ctttaattcc gttcatggtt tcatggtttt
     5101 taaactgcga gggagcgata aaacggaaag gacggggcag ggccagttct tgcaggaaag
     5161 gatagtaggc gcttttcagg gtaatttgca gctctgtttt actgagtgct ttaacatcaa
     5221 caatctggtt tgccagctcc agccaggcgt gacgttgacg gttatcgagc actgcgcgga
     5281 agttttctgc cgccgcctcg gcatcgaacg gttcaccgtt ggagaatttc acgtcatcac
     5341 gcagggtgaa ggtccaggtt ttaccatctt ctgaatgagt ccagcttttt gccagccacg
     5401 ggatcaccga accgtctgcc tgatatttca ccaatggttc ataaaccatg ctctgggcga
     5461 acatctggtt aggcgtgtaa aggtgcgggt ttagtggccc gacattcacc ggccaggcgg
     5521 tggtgatttc atctggtgca gcggcatgga cgataaaaga cgcacaagcc agcagcgcaa
     5581 atagagtgcg gcggagtgtg gagagcatgg taaccccaat ggattaaaat agatggcgga
     5641 aataagtatg acgattttaa gtattcgtca tactgattac ctgttctgga tcaatagatg
     5701 ggcagtcggg tgggcggatc agttaactga atcgatccat tgcacactgt cggcggtgag
     5761 cgtaaaggga gtaggggtgc agatcgcaag gcttaaattt tcaagctgac aatggctgac
     5821 tgacagcgag gagtgatagg tgctgtctac gctgacgatt tgccaggcgc tgccgccacg
     5881 ctgtttaacg atggcttctt tgcgcgtcca gatgcgccag aacatttcca gttgctgatc
     5941 aggatgcacg gcgtccatct cagcgtgttc cccgaggctg aatacggcgt tcgccagcca
     6001 gcgccagttg gcgcgcgggc gaatcacttc gatatcgcag ccgacttcgc cttcatcact
     6061 caacagcagg gcgatatcgt caccgctatg gcttaagttg aaccatagcg gcatttccgg
     6121 cgcaaatgca ggtttgcctt gttcgccata gatgatctcc ggtagcgggg aaagcgagtg
     6181 cgaaagcaat gcacgccccg ccagccagcg ttcgcgtcgt ggaccttgcg gtgcttgctc
     6241 gcgtaaaccc ggtggcagtg gagctgcgct taaggtcgaa actttcccca gaactatccg
     6301 atacatatca gggccaacgt ttaatggaaa atgaaagtgc gtatcgtatc acttgtcgcc
     6361 tcatcccggt aaccgacttt tcggtctgcc cggccccagt aaaatcgcca gtttgctacc
     6421 gcctttggtg gtttccatcc agattttaca cacgctggtt aatggcaccg aaagtagcat
     6481 ccctaccggg ccgagcagcc atccccaaat taacaatgaa agaaatacca ccatggtgga
     6541 catccccagg cgatggccca tcatccgtgg ttctaaaata ttgccgatga ccatatggac
     6601 cactaaaaac aatgcgccga ccagaataca ttcgtaaaca ccattaaaca gcagcacctg
     6661 aatcattggc ggtacggcgg aaattaccgc gccgatattg ggcacgtagt tgagcaaaaa
     6721 cgccagtact gcccacatca gcgcaaactg cacccccatc agctccagcc ccagccagac
     6781 gatgacacct gtccataaac tgagtagcgt cttcaatgca agatagtgcg aaacgccttt
     6841 aagtgcgcgg tgtaatcccg cgatgtgaat ctgtggatta ttcagcgcaa aacgcatttt
     6901 gtaagggacg tggcgcactt caaacagcat aaaaactacg gtcatcacca gcaaaagcac
     6961 gctcgccatt gccccggaaa gcccggtcat tagcgctgtg gtgaaggtaa ccactttttc
     7021 cgagtccatc cgctgcagca ttcgctccgg cgacatatgc aaattaagaa aaggcaacat
     7081 ctcctgcaat ttaaaaagtt tgcgcgtcag ctccttatta aacttcggca gcatagagat
     7141 aaattcgtta aacgatgccg ccagtacgcc gaccagcgcg gttagtgcga tcagcatcac
     7201 caccactaca atcgtaatgg caacggggcg ttgtactccc cgacgaataa accaggtgac
     7261 gagcgggttg aggacgatgg caaaaaacag cgccagtaac agctgcacaa tgatatctgc
     7321 cgctgcgtga atgcccgcga ggatcactac cagcgaggcc agcttgagca gaatgtgcat
     7381 gcccgtttta tcgggttgag gggtttccat tggggcttcc ttgtgacttt ttgtattaag
     7441 tgtagcggga gtcacgccag caatattctg attcttactg ctgattttca cgtcagcacg
     7501 tggtaaaaat gaaacactgt tgtaaaaatg tggtgatcct catgcccgaa cccgtagccg
     7561 aacccgcgct aaacggattg cgcctgaatt tgcgcattgt ctctatagtc atgtttaact
     7621 tcgccagcta cctcaccatc gggttgccgc tcgctgtatt accgggctat gtccatgatg
     7681 tgatgggctt tagcgccttc tgggcaggat tggttatcag cctgcaatat ttcgccacct
     7741 tgctgagccg ccctcatgcc ggacgttacg ccgattcgct gggacccaaa aagattgtca
     7801 tcctcggttt atgcggctgc tttttgagcg gtctggggta tctgacggca ggattaaccg
     7861 ccagtctgcc tgtcatcagc ctgttattac tttgcctggg gcgcgtcatc cttgggattg
     7921 ggcaaagttt tgccggaacg ggatcgaccc tatggggcgt tggcgtggtt ggctcgctgc
     7981 atatcgggcg ggtgatttcg tagaacggca ttgtcactta cggggcgatg gcgatgggtg
     8041 cgccgttagg cgtcgtgttt tatcactggg gcggcttgca ggcgttagcg ttaatcatta
     8101 tgggcgtggc gctggtggcc attttgttgg cgatcccgcg tccgacggta aaagccagta
     8161 aaggcaaacc gctgccgttt cgcgcggtgc ttgggcgcgt ctggctgtac ggtatggcgc
     8221 tggcactggc ttccgccgga tttggcgtca tcgccacctt tatcacgctg ttttatgacg
     8281 ctaaaggttg ggacggtgcg gctttcgcgc tgacgctgtt tagctgtgcg tttgtcggta
     8341 cgcgtttgtt attccctaac ggcattaacc gtatcggtgg cttaaacgta gcgatgattt
     8401 gctttagcgt tgagataatc ggcctgctac tggttggcgt ggcgactatg ccgtggatgg
     8461 cgaaaatcgg cgtcttactg gcgggggccg ggttttcgct ggtgttcccg gcattgggtg
     8521 tagtggcggt aaaagcggtt ccgcagcaaa atcagggggc ggcgctggca acttacaccg
     8581 tatttatgga tttatcgctt ggcgtgactg gaccactggc tgggctggtg atgagctggg
     8641 cgggcgtacc ggtgatttat ctggcggcgg cgggactggt cgcaatcgcg ttattactga
     8701 cgtggcgatt aaaaaaacgg cctccggaac acgtccctga ggccgcctca tcatcttaaa
     8761 acttactgaa taaccagcgt attaatgatg ttttctgcgg tggtctgcgc tttttgctga
     8821 tcgtcagcgg gcagcgtaat ttgcatggtc agcagttgat tacccacgtt acccagaata
     8881 acggaagagt acgccgtctg gcctttcgcg gagataatac tgtctaactg ctgcattttg
     8941 tgacctttca gctcaatggc tttattggtt accacttgca gctgcggatc gcggctacgt
     9001 tgctgatctt ccagacgctt cgccagcacc gccagatctt ctttcggatc atcgcccatg
     9061 atgacgatga ctgctttctg cccggtggcg tcggaccaga catgcatgtt attggcctgc
     9121 gttcccagct taccgctctg gtcggtcata tccgctggca gcgagaaact taacttgcca
     9181 tcaagcaggt tgacgggatt cccggtagcg ttactttccg cgaccgaacc ctgcgccgta
     9241 gcgttagtgt ctttatcatc acaggccgca agccccataa ccagcaggcc aattccgaca
     9301 tatttaacca gattgcgcat tgacttcttc ctttcgataa acggccataa cggctcattc
     9361 atccatctta tcacaactct gataacgaac ctttaactcg cctgcaaagc gttgatttcg
     9421 gatttatctg ccagtctttt caacagcata ttgagtaata cgccatacat tggcaggaag
     9481 aaaacgatac tgattaacac tttgaaacag taatcgacca gcgcgatttc catccagtgt
     9541 tcagccataa aggcatccgg gctacgccag aaggcaatga agaaaaaggc cagcgtgtcg
     9601 ctgacgttac cgaacagtgt ggacgctgtc ggtgccagcc accagcggcg actctgacgc
     9661 aggcggttaa aaacgtgcac gtcgaggatt tgccccagcg cgtaggccat gaaactggcg
     9721 gtggcgatac gggcgacaaa caggttgaag tgggcgagtg cgccgaatcc ctgccaggaa
     9781 cccatataga atagcgacga gatgacgtag gagattaata acgcagggat cattaccgcg
     9841 aagataatgc gtcgggccag cggtgcgcca aaaatacgca cggtcaggtc ggtagcaaga
     9901 aaaataaacg gaaagctaaa cgcgccccag gtggtatgga aacccaaaat ggagacggga
     9961 agctgaacca gatagttact ggaggtgatc accagcagat gaaataacga taaccagaac
    10021 aacgccttat agcgttgagt ttgcgagaaa acgttcatat tgtacctttt tgattaacca
    10081 ttggggtgag ggaacccaat acgtacgaca cgtctgttac cttaacataa taacgtgctt
    10141 accctttttt cgagccgccg catgatactg ctttacgttg acaatgcaat ggttaatttt
    10201 cacgcaatcg ttaacctggt ttgcttacgg actcacaggg cgtaaactag cgccgttttt
    10261 ttatgtgatg agaagaaaat gaccgatctc ttttccagcc ctgaccacac actcgacgca
    10321 cttggcctgc gctgcccgga acccgtaatg atggtgcgca aaactgtgcg caatatgcag
    10381 cccggcgaaa cgttgctgat tatcgccgac gatccggcca ctacccgcga tattcctggg
    10441 ttttgtacct ttatggaaca cgaactggtt gcgaaagaga cggatggact gtcttatcgt
    10501 tatttgattc gtaaaggcgg ttgatggggg ctgattggct tcgatgccgc cttttcccct
    10561 caccctaacc ctctccccag aggggcgagg ggaccgatcg cgctcaatgt tgcgatcggt
    10621 ttgccttatc tcctgcgcaa caatcttaac gcattcgctg tcaccagcac cgtcgcaccc
    10681 gtatctgcca gcaccgccag ccatagtccg gtcatcccta aaagcgtggt gacgaggaag
    10741 atccctttca gtcccagcgc aatggtgatg ttctggcgga tattggcgtg agtggcgcgt
    10801 gccagttcaa tcatttgcac caggccgcgc aggtggttat gggttaatgc tgcatcagcg
    10861 gtttccagcg ccacgtctgt gccactgccc attgcaatcc caatggcggc agctttcatc
    10921 gctggcgcgt cgttaatacc gtcaccgacc atcgccagcg gcgcatgttg attcagctcg
    10981 gtcaccgctt tgactttatc ttccggcaac aggcccgctt taaactccag ccccagctcc
    11041 ccggcaattg ccgccgctgc gcgtggatta tcgccggtga ggatcacccc tttgacgccc
    11101 agcgcgttca gttcactgat ggcagttgca gcatcggcgc gcagggtatc ctgtaacgca
    11161 atgacaccaa gcacgtcatc gttacgtact accagcacta ccgtttgccc ggcgctttcc
    11221 agttcgttaa ttaaaccagc aaatgcatca gcgggatgtt tcccggcagc gcaaatcaat
    11281 acgcgctcac cgttaacctg cgcttcaatg ccagacccga ccagcgcccg ctgtgattcg
    11341 gcggcgggaa tggcgagtgc agcaacctgt gcttcgcgca cgatcgcctg cgccagtgga
    11401 tgcgtcgcgc cttgctcgac cgccgccgcc agtgtcagca gttcagattc actaataccc
    11461 gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg tcagcgtacc ggttttatca
    11521 aacgccacct gggtaacacg acctagctgc tccagcgccg caccgccttt aattaacgcc
    11581 ccacgacgtg ccgctgctgc cagcccggag gtaatcgccg cgggcgtgga gataactaac
    11641 gcacacgggc agccaatcag cagcagcgtc agccctttat aaatccactc ttgccagctg
    11701 gcggcaaaca gcagcggcgg caccagcgtt accagcaggg cgacggccat aattgctggc
    11761 gtataaatac ggctgaaacg gtcgataaac cgctcaatgg gggcgcggcg ctcttcggct
    11821 tcttcgatca gtttcagaat ccggtcaatg gcgctggcac ccggttctga cagcacttcc
    11881 agcgttacca gacgttctac gctggtggct cctgcaggaa ctttatcgcc cgttgcgcgc
    11941 tccaccggaa tggattcgcc ggtcagggcg ctttcatcaa aactggcaaa gggcgagagc
    12001 agtttaccgt cggcaggcaa acgcccgccc gccgcaactt caatcacatc gccagggcgc
    12061 aggctgttaa tcgccacctc ttcccgctca ccgttacgca ggcgcgtggc ggtttctggt
    12121 ttcagcgcca ttaacgcgct aacgccctga cgcgcgcggc tggcggccca gccttccagt
    12181 cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt cagccgttgc gccaataaac
    12241 agcgcaccaa tagcagctac gctcattaac gtttcaatgg cgaagtaact gcctgatttg
    12301 atcagccgta atgcctgacg agcaatcggg tacagcccaa ccagcgtggt cgcgataaac
    12361 gccagttgcc cgaacggatg attgaactgc tccagacccc agctgattgc catcatcacg
    12421 attagcgtaa tcagcggcag attctctttc aggcgcgatg cttgtggctc gtcggcggcc
    12481 tgctcatcac gcagggaata gcccgctttt tgcaccgcag attcaacttg tgcacggatg
    12541 tcattgtcgg catcaaccac cagtttttcg gtggcgaaca gtacctgcac ctgattcacg
    12601 cctgcaagct ggcgcacggc attttctacc ttgcgcgcac aggcggcgca gtccatgccg
    12661 ctgactttcc agctatagcg ggtgccggag acgttttcag agagcgttgg cgtgctggaa
    12721 catgcgccgt cgcagcaaca gtcgttggcg ttctgtaccg tggttagcgg tttgaacgcg
    12781 gcaaattgag gggctttctt gccgtgattg tcaggagtcg acatggcatc ctccggttaa
    12841 gttttttctc attaaccgaa ggatacactc tggagtcgac tccagagtca agttttatca
    12901 gagatacagc gagcggacga tgaggaagtg accggcaaag tagcaggccg cagcaatcgc
    12961 gttatccgca cggaagcgtc ggcgatagtg gctacccagc cagacaaagt tactgataaa
    13021 cagcaacgaa gcgccgacaa acgcagagag cgccggagcg gtcggacgga agaaccacag
    13081 ttcacctacc agccacacca tcaccagcgt catgccgata aacgtgcaga taggccaacg
    13141 atactcttcc agccgcgtcc agataatcgc cagtaacagc gcgcccagaa ccagcagtac
    13201 caacggcaga ggccagaaga aagagagcgt catctgactg gcgaaataga tggtgtacag
    13261 caggtgcgag aggaaaaacg cgccgatggc gtacatcaga cgttgacgtg gcaacagggt
    13321 tagcgcatcg cccagcagtg aggcgcacag ccccgccagc accagatagc taatggcgtc
    13381 gaacatcggc gcttgccagg ccagtaacaa caggagaaga agggttaacg gtttaaacac
    13441 ccagcgttgc caggttggcc cacgatacga tgcatccaca gatagccatg cggaaagaca
    13501 gacagcgata aacgaccaaa gcatcttagt tccttgaatt cgttattttt tcgtaagcgg
    13561 aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg acggatgaca acaccataac
    13621 tggcagggta tgcttatttc cgcattttcc gatgagggat aaagatgagc aaaccacctc
    13681 ttttctttat tgttatcatt ggcttaattg tcgtcgccgc atcgtttcgt tttatgcaac
    13741 agcgacggga aaaagctgat aatgatatgg ctccgctcca gcaaaagctg gtggtggtga
    13801 gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg ccagcaggaa gtgactccgg
    13861 caggcaccag tatgcgctat gaggcaagct tcaaaccgca aagcggagga atggagcaaa
    13921 cgtttcgcct cgacgcccag cagtaccacg ccctgacagt gggcgataaa ggtacgctga
    13981 gctataaagg aacgcgcttt gttagctttg ttggcgaaca ataaggttat tttttaactt
    14041 taaatttctt ctgccagacc agcagttcaa acacgccgaa aaggaaaatc cgcacctttt
    14101 cggcggtggt catctgcggg ccatctttcg gtaaagtgga tttcaatagc gccagctgca
    14161 taccatgcat cagcacggta aaaatcagcg ccacgttaac gaaaatattc agcgggcgcg
    14221 ggaagggatg caccaggttg aggattaaaa atccccaaac gcagagcatt aacaaacgtc
    14281 caatattaat cagcatcact ttctccttgt gcttcgcgtt gatacagccg ataagccacc
    14341 tgacccgcca ctttttcccg atgcaatgac cagtttgccg gaacagtggg cagaccgttt
    14401 tcgacttcgc tttcgacata aatcagggct tcgtcagcca gccagccgtt atcttccagt
    14461 aaatttatcg tctcttctaa caagccacgg cggaacggtg gatcgacaaa cactatatta
    14521 tgcggtgtgc ctttttgcgc caggaatgac atcgcgttgc tgttcactac gcgtgcattg
    14581 cctgctttta gtgtcgccag attcttaatt aactgctgag aaactgcacg atccatctca
    14641 atcaacgtgg cctccgcagc gtagcgtgat aacgcttcca gccccagcgc gccgcttccg
    14701 gcgaagcaat ccagacattg ggcgtcaaca atgaccggag ccagccagtt aaataacgtt
    14761 tcgcgtacgc ggtcggtggt ggggcgcaga cctgggctat caggaaccgg tagtttacgg
    14821 cctcgccact gcccgccaat aatgcggatt tggccgctgc cggaatgatt cggttttttc
    14881 atgataaatt gctcaatccg ccagataaca catacttgca ggcggtgatg tgaattaagt
    14941 taagtgatag actatttcat cattttttta gctgctatgt acatagcgtt aacgctgtgc
    15001 catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa gaaaaaaaac gtggcttttt
    15061 ttcctggctg ggctttggtc aaaaagagca gaccccggaa aaagagacag aagttcagaa
    15121 tgaacaatcg gttgtagaag aaatcgttca ggtgcaagag cctgcgaagg cttctgaaca
    15181 agccgttgaa gagcagccgc aggcgcatac tgaagccgag gcggaaactt ttgctgccga
    15241 cgttgtggaa gtcactgaac aggttgctga aagtgaaaaa gcgcagcctg aagcgggagt
    15301 cgttgcacag ccggaaccgg tcgtagaaga aacgccggag ccagtggcta tcgaacgtga
    15361 agagctgccg ttgccggaag acgtcaacgc cgaagaggtt tcgccagaag agtggcaggc
    15421 tgaagcggaa accgtagaga ttgtcgaagc ggcggaagaa gaagcggcta aagaagaaat
    15481 caccgacgaa gagctggaag cacaggcgct ggctgccgaa gcggcagaag aggcggtgat
    15541 ggtggttcct ccggcagaag aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa
    15601 accgaccaaa gaaggtttct tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa
    15661 tctcggttcc ggatttatca gcctgttccg cggtaaaaaa atcgacgatg atctgtttga
    15721 agagctggaa gaacagcttt tgatcgccga tgtgggcgtg gaaaccacac gtaaaattat
    15781 caccaatctg acggaaggcg catcccgcaa gcagcttcgt gatgccgagg cgctctatgg
    15841 cctgctgaaa gaagagatgg gcgagattct ggcgaaagtc gatgagccgc tgaatgttga
    15901 aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac ggtgtgggta aaaccacgac
    15961 gattggtaag ctggcacgtc agtttgagca gcagggtaaa tcggtgatgc tggcggcggg
    16021 cgatactttc cgtgcagcag cggttgaaca gcttcaggtc tggggtcagc gcaacaatat
    16081 tccggtgatt gcccagcata ccggtgcgga ttccgcctct gttatcttcg acgccattca
    16141 ggcagctaaa gcgcgtaata tcgacgtcct gattgccgat accgccggac gcctgcagaa
    16201 caaatcgcac ctgatggaag agttgaagaa aatcgtccgc gtgatgaaga aactcgacgt
    16261 tgaagcgccg catgaagtta tgctgactat cgatgccagc accgggcaga acgcggtaag
    16321 ccaggccaaa ctgttccatg aagccgttgg tttaaccggc atcacgctaa cgaaactgga
    16381 cggcacggcg aaaggcgggg taattttctc ggtggctgac cagtttggta tccctatccg
    16441 ctacattggt gtcggcgaac gtattgagga tttgcgtccg tttaaggcgg acgactttat
    16501 agaggcactt tttgcccgag aggattaaca atgattcgct ttgaacatgt cagcaaggct
    16561 tatctcggtg ggagacaggc gctgcagggc gttacgttcc atatgcagcc gggtgagatg
    16621 gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc tcctgaagct gatctgtggg
    16681 attgagcggc ccagcgccgg gaaaatctgg tttagcggcc atgacatcac gcgtctgaaa
    16741 aaccgtgaag ttccgtttct gcgccgccag attggcatga ttttccagga tcaccatcta
    16801 ctgatggacc gtactgtcta cgataacgtg gcgatcccgc tgattatcgc tggtgccagc
    16861 ggtgacgata ttcgtcgccg ggtgtcggcg gcactggata aagtcgggct actggacaaa
    16921 gcgaagaact tccctattca gctttcgggc ggtgaacaac agcgtgttgg cattgcccgc
    16981 gcggtggtga ataagcccgc ggtactgctg gcggacgaac cgactggtaa cctggacgac
    17041 gcgttgtcgg aagggattct acgtctgttt gaagagttta accgcgttgg ggtaaccgta
    17101 ttgatggcaa cgcacgacct caacctgatt tcacggcgtt cctatcgcat gctcaccctg
    17161 agcgatggtc acttgcatgg aggcgtgggc catgaataag cgcgatgcaa tcaatcatat
    17221 tcggcagttt ggcgggcgtc tggatcgctt ccgtaaatcg gtcggcggct caggcgatgg
    17281 cggtcgtaac gcaccgaaac gcgcgaaatc ctcgccaaaa ccggtaaatc gcaaaaccaa
    17341 cgttttcaac gaacaggtgc gctatgcctt ccacggcgca ttgcaggatc tgaaaagcaa
    17401 accgttcgcc acatttttaa cggtgatggt tatcgccatc tccctgacgc tgcccagcgt
    17461 ctgttatatg gtgtacaaaa acgttaacca ggcggcgacg cagtattatc cgtcaccgca
    17521 aatcactgtt tatctgcaaa aaacgctgga cgatgacgct gctgcgggcg tggtggcaca
    17581 gttgcaggcc gagcaaggcg tggagaaagt gaattatctt tctcgtgaag acgcgctggg
    17641 cgagttccgt aactggtctg gctttggcgg cgcgctggat atgctggaag aaaacccgct
    17701 tccggcagtg gcggtggtga tcccgaaact cgattttcag gggacggaat cactcaatac
    17761 gctgcgtgat cgtatcacgc agattaacgg cattgacgaa gtgcggatgg atgacagctg
    17821 gtttgcccgt ctggcggcgt tgactgggct ggtcgggcgc gtttcggcga tgatcggcgt
    17881 attgatggtg gcggccgtgt tcctcgtcat cggcaacagc gtacgtctga gcatctttgc
    17941 tcgccgtgac tccattaacg tgcagaaact gattggtgcg acggatggtt tcatcctgcg
    18001 cccgttcctg tacggtggcg cactgctggg attttctggc gcattgttgt cactaatttt
    18061 gtcagaaatt ctggtactgc gattgtcatc ggcggttgcg gaagtggcac aggttttcgg
    18121 aacgaagttt gatatcaatg gtttatcgtt cgatgaatgc ctgctgttgc tgctggtatg
    18181 ctcgatgatt ggctgggtgg cagcgtggct tgccacggta caacatttac gccactttac
    18241 gcctgaataa taaaagcgtg ttatactctt tccctgcaat gggttccgta gcagggaaag
    18301 agaccccgtt gtctcttccc ggtatttcat ctctatgtca cattttgtgc gtaatttatt
    18361 cacaagcttg cattgaactt gtggataaaa tcacggtctg ataaaacagt gaatgataac
    18421 ctcgttgctc ttaagctctg gcacagttgt tgctaccact gaagcgccag aagatatcga
    18481 ttgagaggat ttgaatgact gacaaaatgc aaagtttagc tttagcccca gttggcaacc
    18541 tggattccta catccgggca gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg
    18601 cgctggctga aaagctgcat taccatggcg atctggaagc agctaaaacg ctgatcctgt
    18661 ctcacctgcg gtttgttgtt catattgctc gtaattatgc gggctatggc ctgccacagg
    18721 cggatttgat tcaggaaggt aacatcggcc tgatgaaagc agtgcgccgt tttaacccgg
    18781 aagtgggtgt gcgcctggtc tccttcgccg ttcactggat caaagcagag atccacgaat
    18841 acgtcctgcg taactggcgt atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt
    18901 tcttcaacct gcgtaaaacc aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa
    18961 tggtggcccg tgaactgggc gtaaccagca aagacgtacg tgagatggaa tcacgtatgg
    19021 cggcacagga catgaccttt gacctgtctt ccgacgacga ttccgacagc caaccgatgg
    19081 caccggtgct ctatctgcag gataaatcat ctaactttgc cgacggcatc gaagatgata
    19141 actgggaaga gcaggcggca aaccgtctga ccgacgcgat gcagggtctg gacgaacgca
    19201 gccaggacat catccgcgcg cgctggctgg acgaagacaa caagtccacg ttgcaggaac
    19261 tggctgaccg ttacggcgtt tccgctgaac gtgtacgcca gctggaaaag aacgcgatga
    19321 aaaaactgcg cgctgctata gaagcgtaat ttccgctatt aagcagagaa ccctggatgc
    19381 gagtccgggg tttttgtttt ttgagcctct acaataatca atttcccctc cggcaaaacg
    19441 ccaatcccca cgcagattgt taataaactg tcaaaatagc tattccaata tcataaaaat
    19501 cgggtatgtt ttagcagagt atgctgctaa agcacgggta gtcatgcata aaacgaaata
    19561 aagtgctgaa caacaacatc acaacacacg taataaccag aagaatgggg attctcagga
    19621 tgaacataaa gggtaaagcg ttactggcag gatgtatcgc gctggcattc agcaatatgg
    19681 ctctggcaga agatattaaa gtcgcggtcg tgggcgcaat gtccggtccg gtggcccagt
    19741 acggtgacca ggagtttacc ggcgcagagc aggcggttgc ggatatcaac gctaaaggcg
    19801 gcattaaagg caacaaactg caaatcgtaa aatatgacga tgcctgtgac ccgaaacagg
    19861 cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa atacgtgatt ggtcacctct
    19921 gttcttcatc aacgcagcct gcgtctgata tctacgaaga cgaaggcatt ttaatgatca
    19981 ccccagcggc aaccgcgccg gagctgaccg cccgtagcta tcagctgatc ctgcgcacca
    20041 ccggcctgga ctccgaccag gggccgacgg cggcgaaata tattcttgag aaagtgaaac
    20101 cgcagcgtat tgctatcgtt cacgacaaac agcaatacgg cgaaggtctg gcgcgagcgg
    20161 tgcaggacgg cctgaagaaa ggcaatgcta acgtggtgtt ctttgatggt atcaccgccg
    20221 gggaaaaaga tttctcaacg ctggtggcgc gtctgaaaaa agagaatatc gacttcgttt
    20281 actacggcgg ttatcacccg gaaatggggc aaatcctgcg tcaggcacgc gcggcagggc
    20341 tgaaaactca gtttatgggg ccggaaggtg tggctaacgt ttcgctgtct aacattgcgg
    20401 gcgaatcagc ggaagggctg ctggtgacca agccgaagaa ctacgatcag gttccggcga
    20461 acaaacccat tgttgacgcg atcaaagcga aaaaacagga cccaagcggc gcattcgtct
    20521 ggaccaccta cgccgcgctg caatctttgc aggcgggcct gaatcagtct gacgatccgg
    20581 ctgaaatcgc caaatacctg aaagcgaact ccgtggatac cgtaatggga ccgctgacgt
    20641 gggatgagaa aggcgatctg aaaggctttg agttcggcgt atttgactgg cacgccaacg
    20701 gcacggcgac cgatgcgaag taatcattaa tcggcaactt tgggttgccg ccaaattgct
    20761 aatatcgagt acgttgcttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    20821 tccaaagaaa tcagtaaatt gcaacacaca ttgtaggcct gataagcgta gcgcatcagg
    20881 caatttctcg ttggtcatca cttttgaaat caacttgacg attaacgctt ctcccagccg
    20941 ccctgttgcg ccgtaaatcc cagtgcctgc ataaacgccg tcatcacacc gcgatcttcc
    21001 acgcctgcat ccgccatcca ccagcatgaa acgccaggat tgttacgcaa aacctcttcc
    21061 agcagatatt gccccacacc gcgacgtcgg gtgacttccc gcacacgcag ggaatccagt
    21121 gctccctcgg tgccgcttaa ggttacccgt acggcagcga gcaggcgctc gttaaaacgc
    21181 gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg aaggggaata ctccggccag
    21241 atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc
    21301 ttcatgggta acccgtgtaa atcacaaaag tggaaccagt gtagcgaaat aatttaatcg
    21361 gaggctttct cttttttatt tcttttggca ggtgattaat tttttaacag caataattac
    21421 aaagttaaaa cattagagaa tgaaaaatac ccagcataat cccctgaatg gtagtgaatt
    21481 attccgccct ttgtgccgtt attttatgct gacaaaggca cttttttctg tttatctatc
    21541 aataaattca gaatattatc tgttcttaat cgactgaaaa atagggattt taatcgctag
    21601 tatcacaaaa tactgcgcta aacccttaat cagacaggca aaaacagtgc agtataaaaa
    21661 aagaacagtc tgatttgtta acacataaaa acaaagcaac acaacatcac gaatggggat
    21721 ttttgactat gaaacggaat gcgaaaacta tcatcgcagg gatgattgca ctggcaattt
    21781 cacacaccgc tatggctgac gatattaaag tcgccgttgt cggcgcgatg tccggcccga
    21841 ttgcccagtg gggcgatatg gaatttaacg gcgcgcgtca ggcgattaaa gacattaatg
    21901 ccaaaggggg aattaagggc gacaagctgg ttggcgtgga atatgacgac gcctgcgacc
    21961 cgaaacaagc cgttgcggtc gccaacaaaa tcgttaatga cggcattaaa tacgttattg
    22021 gtcatctgtg ttcttcttct acccaacctg catcagatat ctacgaagac gaaggtattc
    22081 tgatgatctc gccgggagcg accaacccgg agctgaccca acgcggttat caacacatta
    22141 tgcgtactgc cgggctggac tcttcccagg ggccaacggc ggcaaaatac attcttgaga
    22201 cggtgaagcc ccagcgcatc gccatcattc acgacaaaca acagtatggc gaagggctgg
    22261 cgcgttcggt gcaggacggg ctgaaagcgg ctaacgccaa cgtcgtcttc ttcgacggta
    22321 ttaccgccgg ggagaaagat ttctccgcgc tgatcgcccg cctgaaaaaa gaaaacatcg
    22381 acttcgttta ctacggcggt tactacccgg aaatggggca gatgctgcgc caggcccgtt
    22441 ccgttggcct gaaaactcag tttatggggc cggaaggtgt gggtaacgca tcattgtcga
    22501 atattgccgg tgatgctgcc gaaggcatgt tggtcactat gccaaaacgc tatgaccagg
    22561 atccggcaaa ccagggcatc gttgatgcgc tgaaagcaga caagaaagat ccgtccgggc
    22621 cttatgtctg gatcacctac gcggcggtgc aatctctggc gactgccctt gagcgtactg
    22681 gcagcgatga gccgctggcg ctggtgaaag atttaaaagc taacggtgca aacaccgtga
    22741 ttgggccgct gaactgggat gaaaaaggcg atcttaaggg atttgatttt ggtgtcttcc
    22801 agtggcacgc cgacggttca tccacggcag ccaagtgatc atcccaccgc ccgtaaaatg
    22861 cgggcgggtt tagaaaggtt accttatgtc tgagcagttt ttgtatttct tgcagcagat
    22921 gtttaacggc gtcacgctgg gcagtaccta cgcgctgata gccatcggct acaccatggt
    22981 ttacggcatt atcggcatga tcaacttcgc ccacggcgag gtttatatga ttggcagcta
    23041 cgtctcattt atgatcatcg ccgcgctgat gatgatgggc attgataccg gctggctgct
    23101 ggtggcggcg ggattcgtcg gcgcaatcgt cattgccagc gcctacggct ggagtatcga
    23161 acgggtggct taccgcccgg tgcgtaattc taagcgcctg attgcgctta tttccgcaat
    23221 cggtatgtcc atcttcctgc aaaactacgt cagcctgacc gaaggttcgc gcgatgtggc
    23281 gctgcccagc ctgtttaacg gtcagtgggt ggtgggccat agcgaaaact tctctgcctc
    23341 tattaccacc atgcaggcgg tgatctggat tgtcaccttc ctcgccatgc tggcgctgac
    23401 gattttcatt cgctattccc gtatggggcg cgcctgtcgt gcctgcgcgg aagatctgaa
    23461 aatggcgagc ctgcttggca tcaacaccga ccgggtgatt gctttgacct ttgttattgg
    23521 cgcggcgatg gcggcagtgg ctggcgtact gctcggtcag ttctatggcg tcattaaccc
    23581 ctatatcggc tttatggccg ggatgaaagc ctttaccgct gcggtgctcg gtgggattgg
    23641 cagcattcca ggggcgatga ttggcgggct gatcttgggg attgcggagg cgctctcttc
    23701 tgcctatctg agtacggaat ataaggatgt ggtttcattt gccctgctga ttctggtgct
    23761 gctggtgatg ccgaccggta ttctgggtcg cccggaggta gagaaagtat gaaaccgatg
    23821 catattgcaa tggcgctgct ctctgccgcg atgttctttg tcctcgctgg cgtctttatg
    23881 ggcgtgcaac tggagctgga tggcaccaaa ctggtggtcg acagcgcttc ggatgtccgt
    23941 tggcagtggg tgtttatcgg cacggcggtg gtctttttct ttcagctttt gcgacctgct
    24001 ttccagaaag ggttgaaaag tgtttccgga ccgaagttta ttctgcccgc cattgatggc
    24061 tccacggtga agcagaaact gttcctcgtg gcgctgctgg tgcttgcggt ggcgtggccg
    24121 tttatggtgt cacgtgggac ggtggatatc gccactctga ccatgatcta cattatcctc
    24181 ggtctcgggc tgaatgtggt tgttggtctt tctggtctgc tggtgctggg gtacggcggt
    24241 ttttacgcca tcggcgctta cacctttgcg ctgctcaatc actattacgg cttgggcttc
    24301 tggacttgtc tgccgattgc cgggttaatg gcagcggcgg cgggcttcct gctcggcttt
    24361 ccggtgctgc gtctgcgtgg tgattatctg gcgatcgtta cccttggctt cggcgaaatt
    24421 gtgcgcatat tgctgctcaa taacaccgaa attaccggcg gcccgaacgg aatcagtcag
    24481 atcccgaaac cgactttctt cggccttgag ttcagccgta ccgctcgtga aggcggctgg
    24541 gacacgttca gtaatttctt tggcctgaaa tacgatccct ccgatcgcgt catcttcctg
    24601 tatctggtgg cgttgctgct ggtggtgcta agcctgtttg tcattaaccg cctgctgcgg
    24661 atgccgctgg ggcgtgcgtg ggaagcattg cgtgaagatg aaattgcctg ccgttcgctg
    24721 ggcttaagcc cgcgtcgtat caagctgacc gcctttacca tcagcgccgc gtttgccggt
    24781 tttgccggaa cgctgtttgc ggcgcgtcag ggctttgtca gcccggaatc cttcaccttt
    24841 gccgaatcgg cgtttgtgct ggcgatagtg gtgctcggcg gtatgggctc gcaatttgcg
    24901 gtgattctgg cggcaatttt gctggtggtg tcgcgcgaat tgatgcgtga tttcaacgaa
    24961 tacagcatgt taatgctcgg tggtttgatg gtgctgatga tgatctggcg tccgcagggc
    25021 ttgctgccca tgacgcgccc gcaactgaag ctgaaaaacg gcgcagcgaa aggagagcag
    25081 gcatgagtca gccattatta tctgttaacg gtctgatgat gcgcttcggc ggcctgctgg
    25141 cggtgaacaa cgtcaatctt gaactgtacc cgcaggaaat cgtctcgtta atcggcccta
    25201 acggtgccgg aaaaaccacg gtttttaact gcctgaccgg tttctacaaa cccaccggcg
    25261 gcaccatttt actgcgcgat cagcatcttg aaggtttacc ggggcagcaa attgcccgca
    25321 tgggcgtggt gcgcaccttc cagcatgtgc gtctgttccg tgaaatgacg gtaattgaaa
    25381 acctgctggt ggcgcagcat cagcaactga aaaccgggct gttctctggc ctgttgaaaa
    25441 cgccttcttt ccgtcgcgcc cagagcgaag cgctcgaccg cgccgccacc tggcttgagc
    25501 gcattggttt gctggaacac gccaaccgtc aggcgagtaa cctggcctat ggtgaccaac
    25561 gccgtctgga gattgcccgt tgcatggtga cgcagccgga gattttaatg ctcgacgaac
    25621 cggcggcagg tcttaacccg aaagagacga aagagctgga tgagctgatt gccgaactgc
    25681 gcaatcatca caacaccact atcttgttga ttgaacacga tatgaagctg gtgatgggga
    25741 tttcagaccg aatttacgtg gtcaatcagg ggacgccact ggcaaacggt acgccggagc
    25801 aaatccgtaa taacccggac gtgatccgtg cctatttagg tgaggcataa gatggaaaaa
    25861 gtcatgttgt cctttgacaa agtcagcgcc cactacggca aaatccaggc gctgcatgag
    25921 gtgagcttgc atatcaatca gggcgagatt gtcacgctga ttggcgcgaa cggggcgggg
    25981 aaaaccacct tgcttggtac gttatgcggc gatccgcgcg ccaccagcgg gcgaattgtg
    26041 tttgatgata aagacattac cgactggcag acggcgaaaa tcatgcgcga agcggtagcg
    26101 attgtcccgg aagggcgtcg cgtcttctcg cggatgacgg tggaagagaa cctggcgatg
    26161 ggcggcttct ttgccgaacg tgaccagttg caggagcgca taaagtgggt gtatgaactg
    26221 tttccacgtc tgcatgagcg ccgtgttcag cgggcgggca ccatgtccgg cggtgaacag
    26281 cagatgctgg cgattggtcg cgcgctgatg agcaatccgc gtttgctgct acttgatgag
    26341 ccatcgctcg gtcttgcgcc gattatcatc cagcaaattt tcgacaccat cgagcagctg
    26401 cgcgagcagg ggatgactat cttcctcgtc gagcagaacg ccaaccaggc gctaaagctg
    26461 gcggatcgcg gctacgtgct ggaaaacggc catgtggtgc tctccgatac cggcgacgtg
    26521 ctgctggcga acgaagcagt gagaagtgcg tatttaggcg ggtaataaca cgttgattga
    26581 taaggagtca aaagactcct ttgagacagg tgacaaatgt aaaattgcct gatgcgctgc
    26641 gcttatcagg cctactgggt gagttgcaat atattgaatt tgcacgatct tgtaggccgg
    26701 ataaggcgtt tacgccgcat ctggcatgaa acgatgagta atctgtagag tttgattcag
    26761 accttctatt tttccgctta tccgtgcccc atctcccatt ttccctcacc cacgccgtca
    26821 ccgccttgtc atctttctga caccttacta tcttacaaat gtaacaaaaa agttattttt
    26881 ctgtaattcg agcatgtcat gttaccccgc gagcataaaa cgcgtgaatt cgcgcattcg
    26941 gtacaacaag agagataaac gatgaaaccg ttacattata cagcttcagc actggcgctc
    27001 ggactggcgt taatggggaa tgcacaggca gtgacgacca ttccgttctg gcattctatg
    27061 gaaggggaac tgggtaaaga ggtggattct ctggcccaac gttttaacgc cgaaaatccg
    27121 gattacaaaa ttgtaccgac ctataaaggc aactacgaac agaatttaag cgcggggatt
    27181 gccgcatttc gtaccggcaa cgctccggct attttgcagg tttatgaagt tggcaccgcc
    27241 accatgatgg catcgaaagc cattaaaccg gtatatgacg tgtttaaaga ggcggggatt
    27301 caattcgatg agtcgcagtt tgtgccgacg gtttccggtt actactccga cagcaaaacg
    27361 ggccacttac tctccctgcc attcaacagc tcgacccccg ttctctatta caacaaagac
    27421 gccttcaaga aagcaggatt agacccggaa caaccgccga aaacctggca ggatctggcg
    27481 gactatgccg cgaaactgaa agcctccggt atgaagtgcg gctacgccag cggctggcag
    27541 ggctggatcc aactggaaaa ctttagcgcc tggaacggtc tgccgtttgc cagcaaaaac
    27601 aacggctttg acggcacgga cgcggtgctg gagttcaaca agccggagca ggtgaaacac
    27661 atcgccatgc tcgaggagat gaacaagaag ggcgacttca gctacgtcgg tcgtaaggat
    27721 gaatccaccg agaagttcta taacggtgat tgcgcgatga ccaccgcctc ttccggttct
    27781 cttgccaaca ttcgcgagta cgccaaattt aactacggcg taggcatgat gccttacgac
    27841 gccgatgcga aagatgcgcc acaaaacgcc attatcggcg gagccagcct gtgggtgatg
    27901 cagggtaaag ataaagaaac gtataccggt gtggcgaagt tcctcgattt cctcgcgaag
    27961 ccagaaaacg ctgccgagtg gcatcagaaa accggttatc tgccaatcac caaagcagcg
    28021 tatgacctga cccgtgagca gggcttttat gagaaaaacc caggggcgga taccgcgacg
    28081 cgtcagatgc tgaataagcc gccgttgccg ttcaccaaag ggctgcgtct gggcaacatg
    28141 ccgcagatcc gcgtgattgt ggatgaagag ctggagagcg tgtggaccgg taagaagaca
    28201 ccacagcagg cactggatac cgccgttgag cgtggaaatc agttgctgcg ccgctttgag
    28261 aaatcgacga agtcttaatc agtgtaatgt cggatgcgtt tcgcttatct gacctggcat
    28321 cgcgtgtagg ccggataagc gaagcgcatc cggcacagtt caggaattaa ccgtaatgtc
    28381 atcatcccgt ccggtgttcc gctcgcgctg gctgccttat ctgctggtcg cgccgcagct
    28441 catcatcacc gttatctttt ttatctggcc tgcgggcgaa gcgttgtggt actcgctaca
    28501 aagcgtcgat ccgtttggtt tctccagcca gtttgtcggc ctggataact tcgtcacgct
    28561 gttccatgac agctactatc tcgactcctt ctggacgacg ataaaattca gcacctttgt
    28621 caccgtcagc ggtttgctgg tatcgctgtt ctttgcggcg ctggtggagt acatcgtgcg
    28681 cggcagccgt ttctatcaaa ccttaatgtt gctgccttac gccgtggctc ccgccgttgc
    28741 cgccgtattg tggatcttcc tgtttaaccc cggtcgcggg ctgatcaccc attttctcgc
    28801 ggagttcggc tacgactgga accacgcgca aaacagcggt caggcaatgt ttctggtggt
    28861 gtttgcctca gtatggaagc aaatcagcta caacttcctg ttcttctatg ccgcgctgca
    28921 atccattccc cgttcgttga tcgaagccgc agccatcgac ggtgcaggcc caattcgccg
    28981 cttctttaag attgcgctgc cgcttatcgc tccggtgagt ttcttcctgc tggtggtgaa
    29041 cctggtgtat gccttcttcg acaccttccc ggtgatcgac gccgccacgt ccggcgggcc
    29101 ggttcaggcc accacgacgc tgatttataa gatctaccgc gaaggtttta ccggactgga
    29161 tctggcttcg tctgccgcac agtcggtggt gttgatgttc ctcgtcatcg tgctgacggt
    29221 ggtgcagttc cgctatgttg aaagcaaggt gcgttaccaa tgattgagaa ccgtccgtgg
    29281 ctgacgatat tcagccatac catgctgatc ctcgggatcg cggtgatcct cttcccgctg
    29341 tacgtggcgt ttgtcgcggc gacgctggat aaacaggccg tctatgccgc gccgatgacg
    29401 ctcatccccg gcacacatct gctggaaaac atccacaaca tctgggtgaa cggggtaggc
    29461 acgaatagcg cgccgttctg gcggatgttg cttaacagct ttgtgatggc gttcagcatt
    29521 acgctcggca aaattaccgt ctcgatgctc tcggcatttg ccattgtctg gtttcgtttt
    29581 ccgctacgta acctcttctt ctggatgatt tttatcaccc tgatgctgcc ggttgaagta
    29641 cgtatcttcc cgacggtgga agtcatcgcc aacctgaaga tgctcgacag ctacgccggt
    29701 ttaacgctgc cgctgatggc ctcggcgacc gccacgtttc tgtttcgcca gttctttatg
    29761 acgctgccgg atgaactggt ggaagcggcg cggatcgacg gcgcgtcacc aatgcgtttc
    29821 ttttgcgaca tcgtttttcc gctctccaaa accaatctgg cggcgctgtt tgtgatcacc
    29881 tttatttacg gctggaacca gtatttgtgg ccgttgttga ttattaccga tgtggatctc
    29941 ggcaccaccg tggcagggat caaagggatg atcgctacag gcgaaggcac cacggaatgg
    30001 aactcagtga tggcggcgat gttgttaacg cttatccctc cggtggtgat tgttttagtg
    30061 atgcagcgtg ccttcgtgcg cggcctggtc gatagtgaga aataagatgg caggactgaa
    30121 attacaggca gtaaccaaaa gctgggatgg taaaacccag gtcattaaac cgctgaccct
    30181 tgatgtggcg gatggcgaat ttatcgtgat ggtcgggccg tctggctgcg ggaaatcgac
    30241 gctgctgcgc atggttgccg ggctggagcg ggtgacagaa ggcgatatct ggatcaacga
    30301 ccagcgcgtg actgaaatgg agccaaaaga tcgcgggatt gcgatggtgt tccagaacta
    30361 cgcgctttat ccgcatatga gtgtcgaaga aaacatggcg tgggggctga aaattcgcgg
    30421 catgggcaag cagcaaattg ccgagcgcgt taaagaagcg gcgcgcattc tggagctgga
    30481 cggtctgctc aaacgtcgcc cgcgcgagct ttccggcggt cagcgccagc gtgtggcgat
    30541 gggccgcgcg attgtgcgcg atccggcggt gttcctgttt gatgagccgc tctctaacct
    30601 cgatgccaag ctgcgcgtgc agatgcgtct tgaactgcaa cagttgcacc gtcgcctgaa
    30661 aacgacttca ctctacgtta ctcacgatca ggttgaagcg atgacgctcg cccagcgagt
    30721 aatggtgatg aacggcggcg ttgccgaaca gattggcaca ccagttgaag tctacgaaaa
    30781 gcccgccagc ctgtttgtag cgagttttat cggcagtccg gcgatgaatc tgctgacagg
    30841 ccgcgtgaat aacgaaggca cgcatttcga actggacggc ggtattgagc tgccgctaaa
    30901 cggtggctac cgtcagtatg ccgggcgtaa aatgactctc ggcattcgcc cggaacatat
    30961 tgcgctaagc tcgcaggcag aaggcggcgt accgctggtg atggacacgc tggagatcct
    31021 cggcgcagat aacctggcgc acggacgctg gggcgagcag aagctggtgg tgcgactggc
    31081 gcatcaggag cgcccgacgg caggcagcac gctgtggctg catctggcgg aaaatcagct
    31141 gcatcttttt gatggtgaaa caggacaacg agtatgagta actggcctta tccccgcatc
    31201 gtcgctcatc gtggcggcgg taagctggcc ccggaaaaca ccctggcggc aatcgacgtc
    31261 ggggcaaaat acggtcataa gatgatcgaa tttgacgcga agttatcgaa agatggcgag
    31321 atcttcctgc tccatgacga caatctcgaa cgcaccagca acggctgggg cgtcgcgggt
    31381 gaactgaact ggcaggattt actgcgcgtg gatgcgggca gttggtacag caaagcgttt
    31441 aaaggtgagc cgctgccgtt gctttcgcag gtggcggaac gctgtcgtga acacgggatg
    31501 atggcgaata tcgaaatcaa acccaccact ggcactggac cattaacggg caaaatggtg
    31561 gcgctggcgg cacgccaact gtgggccggt atgacgccgc cgctgctgtc atcgtttgag
    31621 attgatgctt tagaagccgc acaacaggca gtaccggaac tgccgcgcgg tttgttgctg
    31681 gatgagtggc gcgacgactg gcgcgaactg accgcgcggc tgggctgcgt ctctattcat
    31741 ctcaatcata agttactcga taaagtgcga gtgatgcagt tgaaagacgc cggactgcgg
    31801 attctggttt ataccgtcaa taaaccccag cgcgcggcag agttgctgcg ctggggcgtg
    31861 gattgcatct gtaccgatgc gattgacgtg attggtccga actttacggc ccaatagttt
    31921 tcaacggaat gtcaggctgc ggcgtgctgg gctgattcag catgtcgccg tttctcctct
    31981 ccggcagcat atgctgctga ttcaacgaac tatccggatt acggttgctg tttaacatcc
    32041 cgccgttggt gttgggcagc atttgctgcc gggcgggatt tcgctccccc ggctgtgact
    32101 gcaacacccg ctgagaatta ttgtttatct ggttttctaa atgctgctgt tgcaactgag
    32161 tttgcgtttt cagttgctga ttcagcatcc ctttttgctg gatttgctgc gtttgcatct
    32221 gggtttgcat ccgctgctgg ctgggtatct gataccccgg ctggttaggg ttgttcagag
    32281 tattaatggg ctgtgcaaag ccgacaaacg gcaggagtgc cgtaagaatc agaagtcgtt
    32341 tcatcgcgta tcctcctctg aagatatcct ttaagtttac tcgcttcccg acaaaacgat
    32401 gattaattca gagttatata ccaggcttag ctggggttgc cccttaatct ctggagaata
    32461 acgatgataa aaccgacgtt tttacgccgg gtggccattg ctgctctgct ctcaggaagt
    32521 tgttttagcg ccgccgccgc gcctcctgcg ccgcccgtct cgtatggtgt ggaggaagat
    32581 gtcttccacc cggtacgcgc gaaacaggga atggtagcgt ctgtggacgc cactgccact
    32641 caggtggggg tggatattct caaggagggc gggaatgccg ttgatgccgc cgtggcggtg
    32701 ggctacgcgc tggcggtaac gcatccgcag gcagggaatc tgggcggtgg tggttttatg
    32761 ttaatccgct cgaaaaatgg caataccacg gctatcgatt tccgcgaaat ggcacccgcc
    32821 aaagcgaccc gcgatatgtt cctcgatgat cagggcaacc cggacagcaa aaaatcactc
    32881 acttcgcatc tggcttccgg cacaccgggt acggtagcag gtttctcgct ggcgctggat
    32941 aaatacggca ccatgccgct gaacaaagtc gtgcagcccg cgtttaaact ggcacgcgat
    33001 ggttttatcg ttaacgacgc gctggctgac gatctcaaaa cctacggtag cgaagtgttg
    33061 ccgaatcacg aaaacagtaa agctatcttc tggaaagagg gcgagccgct gaaaaagggc
    33121 gacacgctgg tgcaggcgaa cctggcaaag agcctggaga tgattgctga aaacggcccg
    33181 gacgaattct ataaaggcac gattgcggaa cagatcgccc aggagatgca gaaaaacggt
    33241 ggcttgatca ctaaagaaga tttagcggcc tataaagcgg tcgaacgcac tccgataagc
    33301 ggcgattatc gcgggtatca ggtttactcc atgccaccgc catcctccgg cgggatccat
    33361 atcgtacaaa tcctcaatat tctggaaaac ttcgatatga agaaatacgg ctttggcagc
    33421 gccgatgcga tgcaaatcat ggcagaagcg gagaaatacg cctacgccga ccgctcggaa
    33481 tatcttggcg acccggattt tgtcaaagtc ccgtggcagg cactgaccaa taaagcctat
    33541 gccaaatcca ttgccgatca aattgatatc aacaaagcga agccgtccag tgagattcgc
    33601 cctggcaagc ttgcgcctta tgagagtaat caaactaccc attactctgt agtggataaa
    33661 gacggtaacg cggtggcggt gacctatacg ctgaacacca ccttcggtac gggtattgtc
    33721 gcgggcgaga gcggtattct gcttaataac cagatggatg atttctccgc caaaccgggc
    33781 gtaccgaacg tttacgggct ggtgggcggt gatgccaacg ccgtcgggcc gaacaaacgc
    33841 ccgctgtcgt cgatgtcgcc gaccattgtg gtgaaagacg gtaaaacctg gctggttacc
    33901 ggtagcccag gcggtagccg gatcatcact acagtgctgc aaatggtggt gaatagcatc
    33961 gattatggca tgaacgtcgc cgaagcgacc aatgcgccgc gtttccacca tcagtggttg
    34021 ccggacgagc tgcgtgtcga aaaagggttt agcccggata cgctcaagct gctggaagca
    34081 aaaggtcaga aagtggcgct gaaagaggcg atgggcagta cacaaagcat tatggttggg
    34141 ccggacggtg agttgtacgg cgcatccgac ccgcgctcgg tggatgattt aacggcgggg
    34201 tactaaggtt agcggccctc ttcgtgggaa gagggctatt ttgtcagggc aagccgaagg
    34261 tagccttttt tatttcgtaa tcctgtagat attcttccag c
//
LOCUS       DACAPB010000027        33940 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.027, whole genome shotgun sequence.
ACCESSION   DACAPB010000027 DACAPB010000000
VERSION     DACAPB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.027"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..717
                     /gene="ltaE"
                     /locus_tag="IT904_001177"
     CDS             <1..717
                     /gene="ltaE"
                     /locus_tag="IT904_001177"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAP3190990.1"
                     /translation="GSIQPQPIDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTH
                     NGKVLPREYLKEAWEFTRERNLALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSK
                     GLGTPVGSLLVGNRDYIKRAIRWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDN
                     AAWMAEQLREAGADVMRQDTNMLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHL
                     DVSREQLAEVAAHWRAFLAR"
     gene            728..2158
                     /locus_tag="IT904_001178"
     CDS             728..2158
                     /locus_tag="IT904_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3190991.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            2257..3270
                     /locus_tag="IT904_001179"
     CDS             2257..3270
                     /locus_tag="IT904_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP3190992.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVSADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(3267..4097)
                     /locus_tag="IT904_001180"
     CDS             complement(3267..4097)
                     /locus_tag="IT904_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP3190993.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4094..4417)
                     /locus_tag="IT904_001181"
     CDS             complement(4094..4417)
                     /locus_tag="IT904_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP3190994.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4543..5058
                     /locus_tag="IT904_001182"
     CDS             4543..5058
                     /locus_tag="IT904_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP3190995.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAM
                     AGTLRQSIENR"
     gene            5276..6004
                     /gene="artP"
                     /locus_tag="IT904_001183"
     CDS             5276..6004
                     /gene="artP"
                     /locus_tag="IT904_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAP3190996.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            6022..6753
                     /gene="artJ"
                     /locus_tag="IT904_001184"
     CDS             6022..6753
                     /gene="artJ"
                     /locus_tag="IT904_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3190997.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            6760..7476
                     /gene="artQ"
                     /locus_tag="IT904_001185"
     CDS             6760..7476
                     /gene="artQ"
                     /locus_tag="IT904_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAP3190998.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            7476..8144
                     /gene="artM"
                     /locus_tag="IT904_001186"
     CDS             7476..8144
                     /gene="artM"
                     /locus_tag="IT904_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAP3190999.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            8370..9101
                     /gene="artJ"
                     /locus_tag="IT904_001187"
     CDS             8370..9101
                     /gene="artJ"
                     /locus_tag="IT904_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3191000.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(9300..10427)
                     /gene="rlmC"
                     /locus_tag="IT904_001188"
     CDS             complement(9300..10427)
                     /gene="rlmC"
                     /locus_tag="IT904_001188"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAP3191001.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(10468..10956)
                     /locus_tag="IT904_001189"
     CDS             complement(10468..10956)
                     /locus_tag="IT904_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAP3191002.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(11016..11861)
                     /gene="potI"
                     /locus_tag="IT904_001190"
     CDS             complement(11016..11861)
                     /gene="potI"
                     /locus_tag="IT904_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAP3191003.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(11858..12811)
                     /gene="potH"
                     /locus_tag="IT904_001191"
     CDS             complement(11858..12811)
                     /gene="potH"
                     /locus_tag="IT904_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAP3191004.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(12821..13954)
                     /gene="potG"
                     /locus_tag="IT904_001192"
     CDS             complement(12821..13954)
                     /gene="potG"
                     /locus_tag="IT904_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAP3191005.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(14049..15161)
                     /gene="potF"
                     /locus_tag="IT904_001193"
     CDS             complement(14049..15161)
                     /gene="potF"
                     /locus_tag="IT904_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAP3191006.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(15512..15988)
                     /locus_tag="IT904_001194"
     CDS             complement(15512..15988)
                     /locus_tag="IT904_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAP3191007.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(16076..16978)
                     /gene="rimK"
                     /locus_tag="IT904_001195"
     CDS             complement(16076..16978)
                     /gene="rimK"
                     /locus_tag="IT904_001195"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAP3191008.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(17039..17761)
                     /gene="nfsA"
                     /locus_tag="IT904_001196"
     CDS             complement(17039..17761)
                     /gene="nfsA"
                     /locus_tag="IT904_001196"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAP3191009.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(17745..18032)
                     /locus_tag="IT904_001197"
     CDS             complement(17745..18032)
                     /locus_tag="IT904_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAP3191010.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18192..18449
                     /gene="grxA"
                     /locus_tag="IT904_001198"
     CDS             18192..18449
                     /gene="grxA"
                     /locus_tag="IT904_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAP3191011.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNEHDDFQYHYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(18479..18856)
                     /locus_tag="IT904_001199"
     CDS             complement(18479..18856)
                     /locus_tag="IT904_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191012.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            19126..20811
                     /locus_tag="IT904_001200"
     CDS             19126..20811
                     /locus_tag="IT904_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAP3191013.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(20986..21522)
                     /locus_tag="IT904_001201"
     CDS             complement(20986..21522)
                     /locus_tag="IT904_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3191014.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVAKPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            21606..22814
                     /locus_tag="IT904_001202"
     CDS             21606..22814
                     /locus_tag="IT904_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191015.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGMGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            22814..23629
                     /gene="ybjI"
                     /locus_tag="IT904_001203"
     CDS             22814..23629
                     /gene="ybjI"
                     /locus_tag="IT904_001203"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAP3191016.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            23715..23999
                     /locus_tag="IT904_001204"
     CDS             23715..23999
                     /locus_tag="IT904_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191017.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(24040..25272)
                     /gene="mdfA"
                     /locus_tag="IT904_001205"
     CDS             complement(24040..25272)
                     /gene="mdfA"
                     /locus_tag="IT904_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAP3191018.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            25557..26153
                     /gene="ybjG"
                     /locus_tag="IT904_001206"
     CDS             25557..26153
                     /gene="ybjG"
                     /locus_tag="IT904_001206"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP3191019.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAVV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            26211..26969
                     /gene="deoR"
                     /locus_tag="IT904_001207"
     CDS             26211..26969
                     /gene="deoR"
                     /locus_tag="IT904_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAP3191020.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(27016..28218)
                     /gene="dacC"
                     /locus_tag="IT904_001208"
     CDS             complement(27016..28218)
                     /gene="dacC"
                     /locus_tag="IT904_001208"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP3191021.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRVWDFVMMKFH
                     QWFGSWFS"
     gene            28465..29091
                     /locus_tag="IT904_001209"
     CDS             28465..29091
                     /locus_tag="IT904_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP3191022.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(29088..30203)
                     /locus_tag="IT904_001210"
                     /pseudo
     CDS             complement(29088..30203)
                     /locus_tag="IT904_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
     gene            complement(30314..30697)
                     /gene="bssR"
                     /locus_tag="IT904_001211"
     CDS             complement(30314..30697)
                     /gene="bssR"
                     /locus_tag="IT904_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAP3191023.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            30910..32235
                     /gene="rimO"
                     /locus_tag="IT904_001212"
     CDS             30910..32235
                     /gene="rimO"
                     /locus_tag="IT904_001212"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAP3191024.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            32461..32847
                     /locus_tag="IT904_001213"
     CDS             32461..32847
                     /locus_tag="IT904_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP3191025.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            32837..33166
                     /locus_tag="IT904_001214"
     CDS             32837..33166
                     /locus_tag="IT904_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP3191026.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(33236..>33940)
                     /locus_tag="IT904_001215"
     CDS             complement(33236..>33940)
                     /locus_tag="IT904_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP3191027.1"
                     /translation="AYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVR
                     RVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFD
                     EDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRF
                     GGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADE
                     RLYKSKKNGRAQISWQ"
BASE COUNT         8408 a         8851 c         8451 g         8230 t
ORIGIN      
        1 ggcagtattc agccgcaacc catcgacgcg gctgccgacg gcacgctacc gctggataaa
       61 gtggcgatga aaatcaaacc cgacgatatc catttcgccc gcaccaaatt actcagtctg
      121 gaaaacaccc acaacggcaa agtgctgccg cgtgaatacc tgaaagaagc atgggaattt
      181 acccgcgagc gcaatctggc gctgcatgtt gacggtgcgc gcatctttaa tgccgtggtg
      241 gcttacggct gcgaactgaa agagatcacg caatattgtg attcgttcac catttgcctg
      301 tcgaaaggtc ttgggacgcc agtcggttca ttactcgtcg gtaatcgtga ttacattaaa
      361 cgtgccatcc gctggcggaa aatggccggt ggcgggatgc gtcagtccgg gattctggct
      421 gccgccggga tgtatgccct gaaaaataac gtcgcacggt tgcaggaaga tcacgacaac
      481 gccgcctgga tggcggagca actgcgtgaa gcaggcgcgg atgtgatgcg tcaggacact
      541 aatatgctgt ttgttcgcgt cggggaagaa aatgctgccg cgttaggcga atacatgaaa
      601 gcgagaaacg tgctgattaa cgcctcgccg attgtccgcc tggtgacgca tcttgacgtc
      661 tcgcgcgaac aactggcgga agtcgccgcc cactggcgcg cattcctggc gcgttaagga
      721 gagagacgtg ccgcaacgca ttttagttct cggtgccagt ggctacattg gtcagcatct
      781 ggtgcgcaca ctcagccagc aagggcatca gatcctggcg gcggcacgtc atgtcgacag
      841 gcttgcaaag ctgcaactgg caaacgtcag ttgccataaa gtcgatctca gctggccgga
      901 taaccttccg gccctgttgc aggatatcga taccgtctat tttctggtgc acagcatggg
      961 cgaaggcggc gattttatcg ctcaggagcg ccaggtggct ctcaacgtcc gcgatgcgct
     1021 acgtgaagta ccagttaagc aattaatctt tctcagttcg ttgcaggccc cgccacatga
     1081 gcagtcggat catctgcgtg ctcgtcaggc tacggcggac attcttcgtg aagcgaatgt
     1141 acctgtgacc gaacttcggg ccggaattat cgttggcgca ggttcagcgg cgttcgaagt
     1201 catgcgcgat atggtctaca acctgccagt gttaacgccg ccacgctggg tacgttcacg
     1261 caccacgccc atcgcgctgg aaaacttgct gcactatctg gtggcgttgt tagatcatcc
     1321 ggccagcgaa caccgcatct tcgaagccgc cggaccagag gtgctcagtt atcagcaaca
     1381 gtttgaacat tttatggcgg tgagcggtaa gcgccgctgg ttgatcccca tccccctccc
     1441 cacccgctgg atttcggtgt ggtttctcaa tgtgattact tccgtaccgc ccaccaccgc
     1501 cagggcgttg attcaggggc tgaaacacga tctgctggcg gatgataccg cgctacgtgc
     1561 actcatccca caacggctga tcgctttcga tgacgcggta cgtagcacgt tgaaagagga
     1621 agaaaaactg gtcaactcca gcgactgggg ctacgacgct caggcctttg cccgctggcg
     1681 accggagtac ggttatttcg ccaaacaggc ggggtttacc gttaaaacgt ccgccagcct
     1741 tgcggcttta tggcaggtgg tgaaccaaat cggcggtaaa gagcgttatt tctttggcaa
     1801 tattttgtgg cagacacggg cgttgatgga ccgcgcgatc ggtcataagc tggcgaaagg
     1861 ccgcccggag cgcgaatatt tacagaccgg cgatgcggtg gatagctgga aagtgattgt
     1921 cgttgaaccg aaaaagcaac ttacgttgtt atttggcatg aaagcgccgg ggctgggacg
     1981 gctgtgtttt accctggaag ataaaggtga ctatcgtact attgatgtcc gcgctttctg
     2041 gcatccgcac ggtatgccgg ggctgtttta ctggttattg atgatccccg cgcatctgtt
     2101 tatttttcgc ggaatggcaa aacaaatcgc cagactggca gaacaaagca cagattaatc
     2161 gacaaaacct gcgttattct ttcatgtttc ccattgcatc atgggggaaa tcacggaaga
     2221 atgcgcacgt cattttctca acaacagtgg attgatatga aggtactggt taccggcgcc
     2281 accagcggct taggtcgaaa cgcggtagag tttttatgcc agaaaggcat cagcgtgcga
     2341 gcgaccggtc gcaacgaggc aatgggcaaa ttgctggaaa aaatgggcgc agagtttgtt
     2401 tcggcggatc tgaccgagct ggtttcatca caagctaaag tgatgctcgc gggcattgat
     2461 acgctgtggc actgctccag ctttacctca ccctggggga cacaacaggc tttcgatctg
     2521 gctaacgttc gcgccactcg ccgcctgggt gaatgggctg tcgcctgggg tgtgcgtaac
     2581 tttattcata tctcttcccc ctccctgtac ttcgattatc accaccatcg cgatattaaa
     2641 gaagattttc gccctcaccg cttcgccaac gagtttgccc gcagcaaagc agccagcgaa
     2701 gaagtgatca atatgctttc gcaggcgaat ccacaaacgc gctttactat tctgcgccca
     2761 caaagtctgt tcggaccgca cgataaagtc tttattcccc gtctggcgca tatgatgcac
     2821 cactacggca gtattctgtt accgcatggc ggcagtgcgc tggtcgatat gacctactat
     2881 gaaaatgccg tgcacgcaat gtggctggca agccaggaag cctgcgataa gctaccttcc
     2941 gggcgtgtgt acaacatcac caacggcgag catcgcacac tgcgcagcat cgtgcagaag
     3001 ctgatcgacg agttgaatat tgactgtcgt attcgttccg tcccctaccc gatgctggat
     3061 atgatcgccc gcagcatgga gcgtttaggc cgcaagtcag caaaagagcc gccgctgacc
     3121 cactacggcg tctccaagtt gaattttgac tttacgctgg atattacgcg tgcgcaggaa
     3181 gagttaggtt atcagccggt catcaccctg gatgaaggta tcgagaaaac tgccgcctgg
     3241 ctacgcgacc acggaaaact gccgcgctaa tcctgcccgt atttctccag caatgcttcg
     3301 gcaatcgcct gagtttctgc atccgcttcg ccgttatata acgtcgggcg gaaatgcatc
     3361 tggaatgcca taatcacgcg ccgctgctcg cgcggtgtca tatcaggttt aacgtcataa
     3421 ccgtagcgcg ccaaaagctc cagcaatgac gcggtatcta ccggtgtgtg cggcgcgcgc
     3481 ccggcaaggt aaaagttaac ccgctgcgca tccggccagg caccaatccc ctgctgcgcc
     3541 agttgctgcc agggaaataa tggccccgga tcgtctttgc gctgcggtgc gatatccgca
     3601 tgtgccacta cgttttccgg cttgatgtga taacgggcaa taatatcttt cgccagtgga
     3661 ataagcgcct gaatctgtgc cggttcaaac ggggcaaaat atttcactcc ggccgatttt
     3721 tgccagccac ggttttccag ctcaatgcca atagaggtgt cgttaaggcg cgttgccccg
     3781 cgccaggcgc taatccccgc atgccaggcc agttcttgtt ccggcaccag ttgccagatg
     3841 cgcggtttac cgttgtatcg cggtggtacc gcagggacca gataatgcga gctgacctgc
     3901 ttatcggtca gtgtcgccag cgagctatca aaatcatctg cggtgtagtg gatcaccagc
     3961 actttaatgc gcggatacgc cgcctgcgcc tggcgtcggg tatcaagctg atatccctct
     4021 ttctcgacaa tgcctttttc gcctgcacac cctgccaata acagagcggc agcgaccagc
     4081 cagaagactc ttctcatcga cgcgttttca ccgccgtacc gctgacgcta accatcagca
     4141 tactgccgtt ttgcccgacc gtttcgtagt cgatatcaat accgacgacg gcatcggccc
     4201 ccagcgcccg cgcctgggag cctaattcct caaaggcgat ctcccgtgct ttacgcagtt
     4261 ctttttcata cgcaccggaa cgtccgccaa cgatatcgcg gataccggca aagaaatcac
     4321 ggaaaatatt ggcacctaaa atcgcttcgc cggtcaccac accgcaatat tcaacgatgg
     4381 tctggccttc cagagttggg gttgtagaaa attgcatgat cgatctcctt gtttgctaag
     4441 ccttcgatct caaaagcatt atcagactga tacgctatta ttgaaaggat atcattatta
     4501 tcgtagaacg ctttcagagc gatcgcttat aaggaaatca ttatgcgcta cagcaaattg
     4561 acaatgctta tcccctgcgc actgctcctc agtgcctgca ccacagtcac tccagcttat
     4621 aaagataacg gcacacgcag tggtccttgc gttgaaggag gcccggataa cgtggcccag
     4681 caattttatg actaccgcat tttgcaccgc agcaacgaca tcaccgcctt gcgcccttat
     4741 ctgagcgaca aactggcgac actgcttagc gatgcctccc gggataataa ccatcgggaa
     4801 ctactgacca acgatccatt ctccagtcga accaccttgc cagacagcgc ccacgttgcc
     4861 agtgcatcga ctatccctaa tcgtgatgcc cgtaacattc ctttgcgcgt ggatcttaaa
     4921 cagggtgacc agggctggca ggatgaagtg ttaatgattc aggaaggcca gtgttgggtc
     4981 atcgacgatg tgcgttactt aggcggtagc gttcatgcca tggcgggtac gctccgtcag
     5041 tccatcgaga atcgctaacg ccttgatcaa ccaataaacc ccgtaaattg tccggcattt
     5101 cgtgaggaat gccgacattt atgctcgccg accaccgccc ccgttatttt gtgctatgtt
     5161 tattgaataa tgcgctttgc ttttaacttt taaagcagaa atattgcata attattctgt
     5221 caaaggtact atcttcgacc tcaatagcta tcagactgcc agtatacgag tgtcaatgag
     5281 tattcaatta aacggcatta attgcttcta cggcgcgcat caggcgctgt tcgatatcac
     5341 gctggattgc ccacagggcg aaacgctggt gttgcttggc cccagcggcg cgggtaaaag
     5401 ctcgctgctg cgtgtactca atctgcttga gatgccgcgc tccggtacgc tcaacattgc
     5461 aggcaaccat ttcgatttca ccaaaacacc ctctgacaaa gcgattcgcg atttgcgtcg
     5521 taacgttggc atggtgtttc agcaatacaa cctgtggccg catctgaccg tgcagcaaaa
     5581 cctgattgaa gcgccctgcc gtgtactggg gttgagtaaa gatcaggcgc tggcccgtgc
     5641 agaaaaactg ctggaacgtc tgcgtctcaa accttatagc gatcgttacc cgctgcatct
     5701 ttctggtggt cagcagcagc gtgttgctat tgcccgtgcg ttgatgatgg aaccgcaggt
     5761 actgctgttc gatgaaccga ccgccgcact ggacccggaa attacggcac aaatcgtcag
     5821 catcattcgt gagctggcag aaacgaatat tacccaggtg atcgtcaccc acgaagttga
     5881 agtggcgcgt aaaaccgcca gccgagtggt gtatatggaa aatggtcata tcgtagaaca
     5941 aggcgacgcg agctgcttta ccgagccgca aaccgaagca tttaaaaact atctctctca
     6001 ctaagattcg ggataacaac aatgaaaaaa gttctgattg ccgcgttaat tgcaggtttt
     6061 agtctttccg ccacagctgc cgaaaccatt cgttttgcta ccgaagcctc ctatcctccg
     6121 tttgaatcga ttgatgcaaa caaccagatc gttggttttg acgtcgacct ggcacaagcg
     6181 ctgtgtaaag agattgatgc aacctgcact ttctctaacc aggcgtttga cagcctgatc
     6241 ccaagcctga aattccgtcg cgtagaagcc gtgatggcgg gcatggatat cactccggag
     6301 cgtgaaaagc aggtgctgtt taccaccccg tactatgaca actctgccct gtttgtgggt
     6361 cagcaaggca aatacaccag tgttgatcag ctgaaaggca aaaaagtcgg cgtacagaac
     6421 gggacgacac accagaaatt cattatggat aagcacccgg aaatcactac cgttccgtat
     6481 gacagctacc agaacgcaaa actggatctg caaaacgggc gtatcgacgg cgtcttcggt
     6541 gacaccgcag tggtcactga gtggctgaaa gataacccga aactggcggc ggtgggcgac
     6601 aaagtgaccg ataaagatta cttcggcact ggcctcggca tcgcggtacg tcagggcaac
     6661 actgagctgc agcagaaact caacactgcg ctggaaaaag tgaagaaaga tggcacttac
     6721 gaaaccatct acaacaaatg gttccagaag taattcctga tgaatgaatt ttttccttta
     6781 gcaagcgccg ccgggatgac cgtcggcctt gccgtttgtg cattgattgt cgggctggcg
     6841 ctggcgatgt tctttgcggt atgggagtcg gcaaaatggc gtcctgtcgc gtgggcaggt
     6901 tcagcgctgg taaccattct gcgtggcctg ccagaaattc tggtggtgct gtttatctat
     6961 tttggctcct cgcagctgct gctgacgctt tcggatggct tcactatcaa tcttgggttc
     7021 gtgcagatcc cagtgcagat ggacattgag aacttcgacg ttagcccgtt cctttgtggt
     7081 gtcatcgctc tgtcactgct gtatgccgcc tatgcctcgc aaacgcttcg gggcgcgttg
     7141 aaagcggtgc cggtgggtca gtgggagtct ggtcaggcgc tggggctgtc gaaatcggct
     7201 atctttttcc gtctggtgat gccgcagatg tggcgtcatg cgctgcctgg cctcggtaac
     7261 cagtggctgg tgctgctgaa agataccgcg ctggtcagtt tgattagtgt gaatgattta
     7321 atgctgcaaa caaaaagcat cgctactcgt acccaggaac catttacctg gtacattgtg
     7381 gcggcggcga tttacctggt gatcaccctg ctcagtcagt acattctcaa acgcattgac
     7441 ctgcgcgcga cacgttttga gcggaggccc agctaatgtt tgagtattta cccgaactga
     7501 tgaaagggct acacaccagc ctgacgctaa ccgttgcctc gctgattgtg gcactgattc
     7561 tggcattgat ttttaccatc atcctgacgc tgaaaacgcc ggtgctggtg tggctggtgc
     7621 ggggttatat cacgctgttt accggtacgc cgctgctggt gcagatcttc ctgatttatt
     7681 acgggccggg ccagtttccg actttgcagg agtatccggc actgtggcat ttgttgtcag
     7741 aaccgtggtt atgtgcgctg attgcgttat cgctgaacag tgcggcgtat accacgcagc
     7801 tgttttacgg tgcaattcgt gcgatcccag aaggtcagtg gcagtcctgt agcgccctgg
     7861 gaatgagcaa aaaagatacg ctggcgatcc tgctgccgta tgcctttaaa cgctcgctct
     7921 cttcttattc caacgaagtg gtgctggtgt tcaaaagtac ctctctggct tacaccatta
     7981 cgctgatgga agtgatggga tacagccagt tgttgtacgg acgcacctac gatgtaatgg
     8041 tgttcggtgc ggcagggatt atttacctgg tcgtcaacgg cctgctgacg ctgatgatgc
     8101 gtctgatcga gcgcaaagca ctggcattcg aacggcggaa ttaatgtagc tgcatgctga
     8161 tgccggatgc ggcgtgaacg ccttatccgg cctacacagt gcataaattc ttataatgaa
     8221 gacggacaac tcactaagtt atccgtcttt ttttatttca tttaaattat ttaatcatat
     8281 ttattgcata taaattcact tgatggcatt gttatcccat gccgcagaca cggccaaaaa
     8341 tcataagatt gacagacggg agttccatca tgaaaaagtt agttcttgcc gctttacttg
     8401 cttcctttac tttcggtgct tctgccgcag agaaaatcaa ttttggcgtt tcagccacct
     8461 atcccccctt tgaatctata ggtgctaata atgagattgt cggctttgat atcgatctgg
     8521 caaaagcctt gtgcaaacaa atgcaggcag aatgtacttt tactaatcac gcgttcgaca
     8581 gcctgatccc gtcactgaaa ttcagaaaat atgacgccgt aatctccggt atggatatca
     8641 cgcccgagcg tagcaagcag gtatcgttta ccacgcccta ctatgaaaac tcagccgtcg
     8701 tgattgccaa aaaagatacc tacaaaacgt ttgccgatct gaaaggcaaa cgtattggga
     8761 tggaaaacgg caccacgcac cagaaatata ttcaggatca gcaccccgaa gtgaaaactg
     8821 tctcctatga cagttatcag aatgccttta tcgatctgaa aaatggtcgt attgatgggg
     8881 tatttggtga cacagcggtg gtaaacgaat ggctgaaaac caatccgcaa ctgggcgttg
     8941 ctactgaaaa agtgaccgat ccgcaatact tcggcaccgg tctgggcatc gctgtacgtc
     9001 cggataacaa agctctgctg gaaaaactga ataacgcgct ggcagcaatt aaagctgacg
     9061 gtacatatca gaaaatcaat gaccagtggt tcccgcagta agattcagaa agccgcaacc
     9121 gcggcttgag actgatgaca aaggtaaaat tgcctgatgc gctacgctta tcaggcctac
     9181 atggcccatg caatgtattg aatttgcacg cttttgtagg ccggataagg cgtttacgcc
     9241 gcatccggca taaacaacgc gcactttgtc agcaatctga agccgcacct gcggcttttt
     9301 tattgcttca ccagcagcgt cagcacttca tagtgcgcgg tgtgcgggaa catatcaaaa
     9361 agctgtaccc gttcgatgcg gtaaccaggc agttctcgga catctttcgc catggtttgg
     9421 gcgttacagc tggagtagat gataaaacgc ggtgccatcg ttgagagata atcacacagc
     9481 ggtttaccaa tgccgcggcg cggcgggtta accagcacca gctccggcac ctccccctga
     9541 gcagtggcaa actgagtgga gtccagcgcc tgaaattgca aacgcgttaa gcccagttca
     9601 gcggctgact gctttgcaca ggcaatggcc tctggcgcaa tttcgatccc ggttaactgc
     9661 atgtcaggcg tcgcgcagtg taaaccaaag ccccccacgc cacagaacag atcccacata
     9721 tgtttaaccg gcagctgtcg tacccagtcg cgcgcggtag cgtacagctg gctggcgacc
     9781 gccggattag tctggaagaa actttgcgga cggacccaca gcggcacatc gttaaaacgc
     9841 tccgccagtg cctgttgttc ggtcaggtag atctccgtct ccccttccat aatcgccata
     9901 tgtaccggct gaatattgac ggtaataact tttagctgcg gtagttgttc ctgtaaccac
     9961 ggtagcgcct tacgcagttg cgccagtttg gtttcggaac gcaacacaaa acgcagcatc
    10021 atgccgccat cgctctggct ttcagtcagc aaaatgtatt tcagttcgcc acgcttacgc
    10081 gccacgttgt agggcgttaa ccccgctcgg gcgataaacg gttttagcgc cgcaaaaacg
    10141 ggcgcaaatg aggcaggata aagcgggcag tcacaaaggt cttccggtgt gccgtctcga
    10201 tgcagcatac cgagcagtgg tttttcaacg ctaccactca ccaccatttt ggctttatta
    10261 cgaaaccctt gttccgggcc tgacaccggc gcgcaccatt cctcaaccgg aaagtcggcg
    10321 agcagatttt taagatcggc ggttttagcg gagagttgct ctggaatcgg ctgcgttatc
    10381 cactgacagg aacgacagcg acccgcgtcg taaagtgcgc actgcataca ttgaccttca
    10441 catcatcagg ggcgacgatt atacacatta ttgcaactgg aagaaccgcc gactggtcga
    10501 gggaacgaac agcagcatca ggatgagcat atccggcagc ttctgcagca tcaggctatg
    10561 gaagatttca cgttttgatt caccgggaat gctgaacagc tccggataac cataccccag
    10621 cgaagccgcc cacaagtaac ttgcggcggt gatttgtgtc agcagatata gccagcgcgc
    10681 ccagcgacgc cctttcacca gtgaaaacgc acaccagatc tcgataaaaa ccagcaccag
    10741 actgcttaaa aagaccagcg ttaagctcca ggtttgtacg ctgcgatgaa tgaactcgcc
    10801 aataccgcgc acgcccagcg tattgaaaat catcagcacg tcgaggccac ggatcataat
    10861 aatggcgagc gccgctacct gcaccagcgc aggcacattc aggcgagcat gagatgaaga
    10921 tgttttctta aaaaatccca acgtttcgtc ttccatgaaa acaatgccgc ggcatgcgcg
    10981 gcattatgta gccaggttgg caaattttag tgtcttcagc cacgtcttgc acgctggata
    11041 tcgcgtatcc gctgtttttc tgcgcgagcc atcagatacc aggcgataaa tccgacaatt
    11101 ccgaccgcac cgagaattaa cgttgcaagg gcgttgattt ccggattcac ccccatccgc
    11161 acgctggaaa agaccagcat cggtaacgtg gtggctcccg gcccagaaac aaagctggcg
    11221 atcaccagat catcaagcga caaagtaaaa gccagtaacc agccagaaat gatcgcgggc
    11281 atgatcatcg gtagcgtaat gacgaaaaat actttcagcg gcgtcgcacc gagatccatc
    11341 gctgcttctt ctatcgagct atccagttcc cgcagacgcg acgaaataac gactgccaca
    11401 taagccgtac agaacgtgac atgcgccagc cagatggtaa gcatcccgcg gtccgcaggc
    11461 cagccaatgg catgagcaag cgcgacgaat aacaacaaca gcgacaagcc cgtgatgaca
    11521 tctggcatca ccagcggcgc ggtgatcata aaggcaaagc catttgatcc gcgaaacctg
    11581 ccaaagcgca ccagcaccac cgccgcaata gtcccgagga tcgccgccgc cgttgccgca
    11641 caggcagcaa ttgtcaggct taaaccaacc gcactcatca tcgcgtcatc gcgcaataac
    11701 tcaccatacc agcgcgttga ccagccggcc cacaccgtca ccagtttcga gctgttgaat
    11761 gaatagatca ccagcatcag cattggcgcg tagagaaagg tgaagcccaa cagcaaaatc
    11821 acaatccgcc agggcgaacg aactaccggt aaattattca tccgtgttct cccacgcttt
    11881 tttgctggtg tttgtgaaac cacattatcg gcacaattag cagcagcaac atgatgatcg
    11941 ctaccgccga ggccaccggc cagtcgcggt tgttaaagaa ctcctgccac agcacgcgcc
    12001 cgatcatgat gctgtccggg ccaccgagca gttccgggat cacaaactcg cccacagccg
    12061 ggataaacac cagcatcgat ccggcaataa tcccaccttt agtcagcggc acgatcacag
    12121 taaagaacgt tttcagcggt cgtgcaccga gatccagcgc tgcttccacc agcgaataat
    12181 caatacgaat caacgcggta taaatcggca gtaccataaa cggcacgtaa gcgtaaacaa
    12241 tgccgatata aacggccaga ttggtatgca gaatggtcag cggttgatcg ataaccccca
    12301 gccacagcag aaaattattc agcacaccgt tgttttttaa tattcccatc caggcataaa
    12361 cgcggatcag aaacgaggtc cacgacggca ggatcaccag tagtaataaa atattacggg
    12421 tcgaaggctt actgtgcgcc accgcccacg ccagcggata gccgatcagt aaacagcaaa
    12481 tagtcgaaat cgccgccacc tgtaacgact ggagataagc atcaaaatag agcggatcgt
    12541 cggtcagttg cagaaaatta ccgagattaa gagtgatgga aagttgcccg tcagcccact
    12601 ccatcagctc ggtataaggt ggaatagcgc gcgccatctc tcccaggctt attttaaaga
    12661 caatcagaaa tggcagcaga aacagcaaga tcaaccagat atatggcaac gcaatgacca
    12721 gtttgcgccc atgcttcatt tgcagctgcg acaaccacag cttaaatccg cccggcggtt
    12781 tcgactgggc agcaggttca agtgtactca tctttgctcc ttaaaccgtc agcaccacac
    12841 agctgtccac ttcccagcac aaacgcactt cgtcgcccca ggtcggtaac cctttacgat
    12901 gacgatgggc gttttgtagc tgggcgctga tcatctgccc acttttcagt cgaacgtgat
    12961 acaccgaaag atcgccgaga taggcaatgt gtatcacctc ccccaccgcg aagttacaac
    13021 cattggcggg cggctcttcg caaagcatga ttttttccgg gcgcagcgct acgtgtaccg
    13081 gcacgttatc gaccactgaa gcatccgcat cgactttcag tggatgcacc agccccggcg
    13141 aatcaagcac caagccatct tcctgacgct ctttgagtac accttcaaag acattaaccg
    13201 aaccgataaa ttcagcgctg tagcgggtag ttggatgctc gtagatctct tccggctcgc
    13261 caatctggac aaatttcccg cgattcataa tggcgatacg ccccgccatg gtcatcgcct
    13321 cttcctgatc gtgggtgacc atcacacaag tcacaccgac gcgctccaga atatccacca
    13381 cttcaagctg catccggtca cgcagctttt tatccagcgc gcccatcggc tcatcgagca
    13441 gtaatagttt cgggcgtttg gcaaggcttc gggccagggc cacacgctgt cgctgaccac
    13501 cggaaagctg atgcggtttg cgtttggcga actcctgcat gtgcaccagg ccgagcatct
    13561 cattgacccg actggcaatt tccgctttcg gtagtttgtc ctgtttcagg ccaaaagcga
    13621 tgttctgttc cacggtcata tgcggaaaca gcgcgtaaga ctgaaacatc atattgatgg
    13681 ggcgcaggta aggcggaacc tgtgacaaat cgacgccatc aagcattatc tgtccggcag
    13741 aaggttgttc gaaacctgcc agcatacgca gcagcgtgga cttgccacag ccggatgcgc
    13801 ccagcagcgc gaagatttca cctttgtaga tagtcaggct gacatcatcc accgcatgtt
    13861 gaccatcgta ggatttggtg aggttgcgga tttctaatag cggcgtcagc gccttacggg
    13921 ttttcgcctg cgggcgaggg atagcgtcat tcacggctgc tctccggcat tgaatgtgtg
    13981 caagcgcacc atgatggtgc gaattgccgg cgtgtgtggt gcacccgtcc ggcatctacg
    14041 actgcggatt attttccgct cttcactttg gtccacgcgc gggtgcgcac acggtcgatt
    14101 ttcgggtcct gcactttcag agtgaacagc ttcgcacgga catccgcagg cggataaata
    14161 cccgggttat cacgaacttc cgcactcacc agcggcgtgg ctgctttatt ggcgttagca
    14221 tagaacacat ggtcagagat atgtgccacc acatccggac gcagcaggta attcaggaac
    14281 tgataggctt cgtctttatt tttggcatcg gcaggcatgg cgaatacatc aaagaacgcc
    14341 atcgcccctt cttttggaat cgagaacgag acattcacgc cattcttcgc ttccttcgcg
    14401 cggtttgacg cctgccagac atcacctgcc caaccaatcg ccacgcagat gtcaccattc
    14461 gccaggtcat taatatattg agatgaatgg aaataacgaa tgttcgggcg cagctttaac
    14521 agcagatcgg tagcaggtcc ggtgtaatca tccgctttag tgctgttggg atctttgccg
    14581 agataattca gcacggtagc aaaaacttct tctggcgcat cgaggaaaga gacaccgcag
    14641 cttttcagtt tttccagatt ttcaggcttg aggatcaagt cccagctatc gacgggcgcg
    14701 ttttcgccca gcaccgcttt gactttatcg acgttatagc caatcccggt cgtcgcccac
    14761 atatagggca tagcaaattt attgtcggga tcgtgtttgg cgaccaactt cagcagttcc
    14821 gggtcgagat tcttccactc cggcaatttg cttttatcca gcggctggaa aactcccgct
    14881 gtcagctggc gttcaagaaa gcttgctgac ggcaccacca ggtcaaagcc ggtgctcccg
    14941 gccattaatt tgccttccag tacttcgtta gagtcgaaaa catcgtagac gactttaata
    15001 ccggtttctt tttcaaagtt agccaccgtg tcgggggcga tataatcaga ccagttataa
    15061 atgtggagtg ttttttgttc agccgcgagc gtgccgacag agacggccat cagagcaccc
    15121 gcaaccagac ccgaaagcca ttttttattt aaggcggtca tatctctttc cttctgaaag
    15181 ttcgttaaca aacgaacaaa agttgtcaca ctgaaagata tgcaataaac gtgcatattt
    15241 tttatcggcg cggtgtaaga aaagagaact ctctcccggc cagcactgct cgctatataa
    15301 aagcatcgca agaataactt tagccagggt ttgagactat agctcggatt aaaaaacggt
    15361 atttaacaaa tttttatgcg tatttgctct atgtgataaa gccaaatcga cacaagagaa
    15421 gtgaaataat ctttaagaaa aggttaaaaa cagcagataa ttttgcagcg cgcagccact
    15481 gcaccagcga ctgcgtgctc gaaaggagtt atcagtggag gaaatagttt tcggtaacgt
    15541 tgttttgccc ttcatcgtca gtcggtaaca gcagttgatg ggcattagct tcaagaatga
    15601 ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc aaactgctca taggttacgc
    15661 cctgcataac ggataaagac tggcaaacca ccagctttgg cagattatca tcctgcatat
    15721 caagaaatgc tttcacggtc agcgaactgg cattgatggc agataaatcc gccgccagcg
    15781 gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa caggatcgtg ttatcgatca
    15841 gatcgatttt ggcatcaaag acaccgtcga aattctgcat atggggcaga tgcagagcct
    15901 gacagttatc acattcaaaa aaactcatcc ccaggtcatc gagccattga cgcagcgtat
    15961 ccagaccagg aacgaccagc gatgtcataa ttttgtacaa cctctttcga taaaaagacc
    16021 ggcacagctt acgcaaaaag cgcaggcaaa accatgatca gtaatgtgat tgcgactaac
    16081 cacccgtttt caggcaatat tctgtcgtag cgtggcgttc gatccagcgg atcattttac
    16141 ccgcgatgtc gataccagtg gttttttcta ttccttccag ccccggcgac gcattcactt
    16201 ccatcaccaa cggcccgcga ttagcacgca gaatatccac accagcaacg tccagcgcca
    16261 tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg tgtgatactg gcgacgcttg
    16321 ccgcgccgcc acgatgcaaa ttggaacgaa aatcgccctc tttcgcccgc cgttcaatcg
    16381 cagcgaccac ttcatcgcca acaaccagac agcggatatc gcacccttgc gcctctttga
    16441 tatattcctg caccagaata tgcgcgttca gaccgcggaa agcgtcaatc acgctttccg
    16501 ccgcctgacg cgtctccgcc agcacgacgc caattccctg cgtgccttca accaacttga
    16561 ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct ggtatcatcc ggcgaatgcg
    16621 caatgcccgt gacaggcagg tcgatgccct gacgcgccag cagttgcatg gaacgcaatt
    16681 tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg atagctcccc agcatctcga
    16741 actggcgcag tgccgccgtc ccataaaagg taatggcggt gccaatacgc gggatcactg
    16801 cgtcaaaatg gggtaacttg cggcctttgt agtgaataga agacgccgca ggatttatgt
    16861 tcatgtagca agaaagcgga tcaagaattt caaccaggtg accgcgctgt atagcggctt
    16921 cacgcagccg cttacacgaa tagagcgttc catcccggga caatatggca attttcaccc
    16981 tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca tacatcgacg tggcggtttt
    17041 agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat aaatgggcgg ctttctttaa
    17101 tgattgttcg gcggatatga tcgctccagg tatcccggcg attattgctg ccacgggtga
    17161 ggtaatattc agccagttgt tcgtcatact gcgccagtgc gtctttatcc agcggttgat
    17221 agctgttttc atgcaccaaa atggaggacg gtaaacgcgg cttaagatcc ggattatccg
    17281 caggccagcc aaggcacagc ccaaacagcg gcagaacatg ctgcggtaat ttcagcagtt
    17341 tcgtcaccgc ttcaatatta ttgcgcaggc cgccgatata tacgccgccc aatcccagcg
    17401 attccgctgc gattaatgca ttctgcgcca tcattgccgt atcaacgaca ccgagcaaca
    17461 gttgttccgc caggccgagc tgagcatccg gacagatctg taaatggcgg ttaaagtcgg
    17521 cacagaacac ccagaactcc gccgcttgcg ctacgtgttt ttgcccgccg gtcagcgtca
    17581 ccagttcttc acgtaacgct ttgtcggtaa tgcgaataat gctactgcac tgcaaaaaac
    17641 tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc acgctgcgct tcggaaatgg
    17701 gttcatcagt gaaatggcga atggagcgat ggccacaaat aagttcaatg gttggcgtca
    17761 ttatcttttt ctctttctga acgtgaatat tgcggtggac ggttcatcag ctgtggggca
    17821 agacgttttg ccacctgaag aataaccacc accgcagcgg gaagcatgag caaaacaccg
    17881 agaaaaatca tcagaatctg cacttctggc cgagaaaatg gctcaggcag cgacagggag
    17941 tcacttaccg acagcagcgc caccgccagt agcatcattc cgataaattc cagtatcaac
    18001 acgcctttag gcaatttacc gatcgcgcgc atacgcttcc ctctgcaaag tgagccttca
    18061 gtctaaaact tttcactgta ttgtgtttaa cagttatagc ttttagcaat taatgcaaca
    18121 ggttaaacct actttcagcg aatacatttt agcgtgatca ttacaggcat aaatctatga
    18181 ggagagaaat aatgcaaacc gttatttttg gtcgtccggg ttgcccttac tgtgtgcgtg
    18241 caaaagatct ggctgagaaa ttgagcaatg aacacgatga ttttcagtat cactatgtag
    18301 atattcgtgc ggaagggatc actaaagaag atctacaaca aaaggcaggt aaacccgtag
    18361 aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg cggctatacc gattttgctg
    18421 catgggtgaa agaaaatctg gacgcctgat cgtctgacaa gccctcgcgt tgagggcttt
    18481 actgattttt tctgtgctgt ggtttaaaca aactactgat aaataagaaa cacagtgccc
    18541 ccagcgcaca ccagaacacc gcacttaata accatgccag ctcttgccag aatgagcgcg
    18601 tcggtgaaaa aaacagccgc ataatgagca tcgaacaggg tgccgccagc attgcgccaa
    18661 acagaggttt caggacttct ctacgctgtg aaaagaagct ggcgactgct ccaggaagaa
    18721 tgaaaaatag caagccgatt tcaggatgcc cggcagcccg aaaagcgcct ttcatgtgcg
    18781 tcgccagaaa aaggcacacc acaatgaaga ggacaaaaca gcagattgcc cccgcccaac
    18841 gttgtttatg tttcactcgt tcctcctgac actgcgtcta tcgaacacat ttttcgccag
    18901 tgtggcgttc agtaagataa agccgcttcg cattccatgc taatataggc caacgcaatt
    18961 catatagccg ttgataccta atgtgattac actagtaaaa tatattgtta ctttactatc
    19021 gtttaggtgc gctgaatgaa tctgcgccct gaattctggt aaaaaacatt atcgtaaatt
    19081 accatttctt tcaacagctt actagtaaac aagaagttag cctccgtgaa tataaacgtc
    19141 gccgaattgt taaatgggaa ttacattctg ttattatttg tggtcctcgc gcttgggcta
    19201 tgtctcggaa agttacgact tggttcgatc caactgggta attccattgg cgttttagtc
    19261 gtatcgctgt tattaggcca acaacatttc agcattaaca ccgatgcgct taatcttggc
    19321 tttatgctgt ttattttctg tgttggggtt gaagccggac cgaacttttt ttccattttt
    19381 tttcgcgatg ggaaaaatta cctaatgtta gcactggtga tggttggcag tgcgctggtg
    19441 atcgccttag ggttaggtaa gctgtttggc tgggatattg gcctgacggc cggtatgtta
    19501 gcaggctcta tgacgtcgac accggttctg gtcggtgctg gcgatacact gcgtcattcc
    19561 ggcatggaaa gcaggcagct ctcactggca ctggataatc tgagcctcgg gtatgcctta
    19621 acctatttaa tcggtctggt gagtttgatt gttggtgcgc gttacttgcc gaaattgcag
    19681 catcaggact tacagaccag cgcccagcaa atcgcccgcg aacgtggcct ggacactgat
    19741 gccaaccgta aggtttattt accggtgatc cgcgcctatc gcgtcggccc ggagctggtg
    19801 gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac cggctgctac
    19861 attgaacgta ttcgacgtaa cgggattctg gcaaatccag acggtgatgc cgtgctacaa
    19921 atgggcgatg aaatagcgtt ggtaggctat cccgacgccc atgcccgact cgatcccagc
    19981 ttccgtaacg gtaaagaagt tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa
    20041 gaagtggtcg ttaaaaacca taacgctgta ggtaaacgtc tcgcacaact gaagttgacc
    20101 gatcacggtt gcttccttaa ccgcgtcatt cgtagccaga ttgagatgcc gatagatgac
    20161 aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg gtgatgcccg tcgcgtaaaa
    20221 accatcgccg atcgcatcgg ctttatctcg attcacagcc aggtcactga cctgctggca
    20281 ttttgcgcct tctttgttat tgggctgatg atcgggatga tcaccttcca gttcagcaca
    20341 ttcagtttcg gcatggggaa cgctgccggg ttgttattcg ccggaattat gctgggcttt
    20401 atgcgtgcta accacccgac cttcggttac attccgcagg gtgcattaag catggtgaaa
    20461 gagttcggct tgatggtgtt tatggcaggc gttggtctga gcgccggtag cggtattaat
    20521 aacggcctgg gcgcgattgg cggtcagatg ttgattgccg gattgattgt cagtctggtg
    20581 cccgtggtta tctgtttctt gttcggtgct tatgtattgc gaatgaaccg cgcgctgttg
    20641 ttcggcgcaa tgatgggcgc acgtacctgc gcgccggcaa tggagatcat cagtgataca
    20701 gctcgcagta acatcccggc gctgggctat gcgggcacct atgcaatcgc caacgtcctg
    20761 ctgacgctgg cagggacaat catcgtcatg gtatggccag gattaggata aaactgaagt
    20821 tgccctgaaa atgaaatttt tttgcacaac cgcagaactt ttccgcaggg catcagtctt
    20881 aattagtgcc actgcttttc tttgatgtcc ccattttgtg gagcccatca accccgccat
    20941 ttcggttcaa ggttgatggg ttttttatta tctaaaattt atctactacc ctgcaaccct
    21001 ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag
    21061 cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc
    21121 gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg
    21181 gctagccagc gcgtagagct ggtacatcag ctccatatta tccggctttg caacctgtga
    21241 gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa catcgctaaa
    21301 aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct ccagcaacaa
    21361 ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg caagggtagc
    21421 aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca gcgtggcctg
    21481 gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctgtctgc
    21541 aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt
    21601 gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta
    21661 tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc
    21721 gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt ctgtcgatcg
    21781 gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg acacgtaatg
    21841 tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt ctggcactct
    21901 ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca agttttggtt
    21961 ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg aataaaacgg
    22021 ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga
    22081 tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg gcgctggtag
    22141 gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc aaaaatgctg
    22201 ccgatggcac ccagcatggc gaaaaaggcg taccttttta tcgcgatatc cagttgctgc
    22261 tgattggtgt tgtggtgctg gcgatggcct ttgccgaagg ttctgccaac gactggttac
    22321 ccttattaat ggttgacggt cacggtttta gccccacttc cggctcgctg atttatgccg
    22381 gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc gaccgttaca
    22441 gtcgcgttgc cgtggttcgg gccagtgcgc taatgggggc gttgggtatt gggctgatta
    22501 tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga atgggtgcct
    22561 cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat gcaccaaccc
    22621 gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg ccgctgctgg
    22681 gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta
    22741 ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga
    22801 tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta ctttcttaag
    22861 cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca
    22921 aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttcttccc
    22981 tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag tgagtgaagg
    23041 caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg tggaacattt
    23101 gctgacgcgc ccggaagtgg aaattattgc ctgcggcaaa aatagcgcct atacgctcaa
    23161 aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt
    23221 cgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc tttccgatga
    23281 actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccagt
    23341 ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag ccaatggcct
    23401 tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct ttggcgatgg
    23461 cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg
    23521 cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg aaggcgtact
    23581 ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa aacggcagca
    23641 cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta acccgctgca
    23701 ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt ttaatgggct
    23761 ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga tacaaagtgg
    23821 ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact gtgccgcaaa
    23881 ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat gctcgccatc
    23941 ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat caccattaat
    24001 tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt gagaatttcc
    24061 catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc cgttgacaag
    24121 gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc tgatttcaat
    24181 accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg tacctttact
    24241 catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac caataccgaa
    24301 agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga taaccgttgc
    24361 cgcagcagcg accaacaggc caatcataat cggccagccg cccataatta tcagcgaacg
    24421 tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta acgccccgaa
    24481 aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg taatgatgat
    24541 aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca gcgccagcgc
    24601 ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag
    24661 tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg gctcgttgca gaccgaaaaa
    24721 ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca gcacatggat
    24781 ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt tcgccatcag
    24841 cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc tgaattgcgg cgtatcccac
    24901 agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagggtga attgttcaat
    24961 gttttgcgcc agcaatattg ccagacaggt gacgataaac cacaccactc ccgccagcat
    25021 caccggacgg cgaccaatac gatccgacag cggccccagc agccattgta aaaacatccc
    25081 gcccgccaga tacgcggtca tcgaagtagg aacccaatca atgcccgcct gatattgttc
    25141 caccacggcc aacataccgg gttgaatcat atcgttgccg atataggttg aaaattcgta
    25201 aagcaccaga cagagaggga aaagtaacgc ctgacgtcca agcctggcac cggaagctaa
    25261 tttattttgc atgcaatttc ttcgccaata ataatcgcgc agagtttaat aaaagcgcag
    25321 ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa gcacattctt acattattgc
    25381 gagtatagct acgctttctt taagttttat ttaacctatg cccgttacaa tcacccaccg
    25441 taaacaggcc gcttgaggga aataagacga tgccgcttta cccagtttaa cctgcacttt
    25501 attctcaacg acttgcctgt attggctccc ttttaatcac tttgcatcgg gaagttatgc
    25561 tggaaaattt gaatctctct ctattctctc ttattaacgc gacgccagac tcggctccgt
    25621 ggatgatctc gttggcgatt tttattgcta aagatttgat taccgtggtg ccgttgctgg
    25681 ccgtggtact ttggttgtgg gggcttacag cacaacggca actggtgata aaaatcgcta
    25741 tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg acatcttttt ccgcacgacc
    25801 gaccctttgt cgaaaatatc ggctataact tcctgcatca tgcggcggat gactcattcc
    25861 caagcgatca cggtacggtg attttcacct ttgcactggc atttttatgc tggcatcgcc
    25921 tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat tgcctggtcg cgcgtttatc
    25981 ttggcgtcca ctggccgctg gatatgctcg gtggattgct ggcaggtatg attggctgcc
    26041 ttagtgccca gattatctgg caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt
    26101 ggtatcgcgt ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc
    26161 taaaaagtgt agtattgagc ggctcgcttc aataactatt cagagggatt atggaaacac
    26221 gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa acgcagcgat aagttacatc
    26281 ttaaagacgc cgccgccctg cttggggttt cggagatgac gattcgtcgc gatctgaaca
    26341 accacagtgc gcccgtcgtt ttgctcggcg gctatattgt tctggaaccg cgcagtgcca
    26401 gccattacct gttaagcgat caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa
    26461 aactggctgc gacgctggta gaacccgatc agaccctctt ttttgactgt ggcaccacca
    26521 cgccgtggat tattgaagcg attgataatg aaatcccttt taccgccgtt tgttattcgc
    26581 taaatacctt tctggcgctg aaagagaaac cccattgccg cgcgtttctt tgcggtggtg
    26641 aatttcacgc cagcaacgcc attttcaaac ccatcgattt tcagcaaacg ctgaataatt
    26701 tttgcccgga tatcgctttt tattctgcgg cgggcgtgca tgtcagtaaa ggcgctacct
    26761 gttttaatct tgaagagttg ccggtaaaac actgggccat gtcgatggcg caaaagcatg
    26821 tgctggttgt cgaccacagt aaatttggca aggtgcgtcc ggcgcgcatg ggtgacctga
    26881 aacgctttga tattgtggtg agcgattgtt gcccggaaga tgagtatgtg aagtacgcgc
    26941 agacgcagcg cattaagttg atgtattaat gacgtataac cggatgacgt ttcgcgccat
    27001 ccggttatca gaagattaag agaaccagct gccgaaccac tgatggaatt tcatcatcac
    27061 gaaatcccac acccgaccaa agaatccgcc ctcttccaca ttttccatca cgatcagcgg
    27121 acgctgctca atggatttac cgttaagctg gaaatcaatg gtcccgacaa cctgaccttt
    27181 tttcagcggt gcggtaagct gcggttccgt taacgtataa ctcgctttca ggtttttcag
    27241 ctgcccacgc ggtatggtca cggagcccgc ttcgcctgcc ccgagattca cttcgctctt
    27301 atcaccaaac cagacgcgct gagtcacaaa ggtggcatca ggtttaattg gcgtcacagt
    27361 ttcaaagaag cggaaacccc aggtcaataa tttctcagac tcattaaaac ggatacggtc
    27421 ggttttcgcc cccagcacta cggagattaa acgcatatcg ccctgggtag ccgaagcaac
    27481 cagattatat cccgcgcctg ccgtggttcc tgtcttcatg ccatcaacat tcagattgct
    27541 gctccacagc agacggttac ggttaggctg acgaattttg ttgaaggtga actctttctc
    27601 tttatgaatg gcgtactctt ccggcacatc gtggatcaat gctttgccca gcaatgccat
    27661 atcgcgcgcg gtactgaact gccccggcgc atccagaccg tgcaccgtct ggaaggtagt
    27721 gttggtcaga cccagttttt tggcataacc attcatcaaa ccaataaatg attcctggct
    27781 cccggcaacg taatcggcca gcgcaataca ggcgtcatta ccggactgga taatcacacc
    27841 tttgttcaag tctgccaccg aaacctgatc gcccggtttg aggaacatta ccgatgaacc
    27901 acgcagtgcc ggatttcccg tcgcccaggc atctttaccg accgtcacca tatcggtgag
    27961 tttaatctta tcggccttaa gcgcctgccc aaccacatag ctggtcatga ttttagtcag
    28021 gctcgcggga tccagtttct catccgcgtt gccttctgcc agcactttac cgctggcgta
    28081 atccattaaa atccatgcac gcgcatccac gctcggcgct tcaacggttt gttccgccgc
    28141 gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag
    28201 agaggagtat tgcgtcataa atgccacccg agtatccatt caaaaaaaat acgttatatc
    28261 gccgtatgat aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg
    28321 gtaaagtttt taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt
    28381 tgccagctgt tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagcctgtgg
    28441 ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac
    28501 gtaaaaaaag tattgctgac gctcgaagaa ctggaactac cttatgagca aattctcgcg
    28561 ggccgtgagt ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg
    28621 gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc
    28681 cgctatctgg cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt
    28741 gcggaagcgg aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg
    28801 atcctgatgg gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc
    28861 agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa
    28921 tggttctctg gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat
    28981 ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa
    29041 ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg
    29101 ctaactttaa gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt
    29161 ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc
    29221 actttgtcgc cgttgacgct catcacaatg acatctttat ctttcagcgc gccaataaat
    29281 aatttttgct gctactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg
    29341 ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct
    29401 tccggtatct taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt
    29461 ttgccttttt gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac
    29521 agtgcattac tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag
    29581 atctcggcgc gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg
    29641 tcggtcagac gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga
    29701 ttgttttcgc ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca
    29761 aaatggttgc cggtagacag ttttggcatc tggcgaaaga cggtgcggaa gtcggtcact
    29821 tttgagagat catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg
    29881 ccaacttcgg aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc
    29941 acgtccagca ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca
    30001 gataatcctt ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata
    30061 ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact
    30121 tcgacattta ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca
    30181 aggaaaaagg attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca
    30241 gtggaggaga taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc
    30301 tcccccttag ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca
    30361 gcgttaagct catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc
    30421 aatcatggtg cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat
    30481 gaagagcact ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca
    30541 gttcgcgatt cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt
    30601 atccttttgc cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc
    30661 ggttcagcag atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc
    30721 cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa tgctgtccag gatcaattct
    30781 tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat
    30841 acaaatttta tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt
    30901 ttttaagcta tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt
    30961 ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac
    31021 gtggtaccga gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac
    31081 agcgcggtac aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta
    31141 attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg
    31201 ctggaaatca ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg
    31261 ccaaaaccga aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg
    31321 ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc
    31381 attattccgt ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa
    31441 gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc
    31501 gcctatggcg ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc
    31561 agcatggtca gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac
    31621 gtttaccctt atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg
    31681 ccgtatctgg acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt
    31741 ccgggttctg tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa
    31801 ctgaccctac gctcaacctt tattgtcggc ttccctggcg agacagaaga agatttccag
    31861 atgctactcg acttcctgaa agaagcgcgt ctggatcgcg ttggctgctt taaatacagc
    31921 ccggttgaag gtgcagacgc caatgccctg cctgaccagg ttccggaaga agttaaagaa
    31981 gaacgctgga accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag
    32041 aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt
    32101 cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac
    32161 gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg
    32221 ggtagccggg tttaattaat ctaaaggcag atctaacaag gatattgttc ttcgatctgc
    32281 ctctactacc cttcccgatg tcctaccctt acgcacaaaa tcccctgacg cataaatcga
    32341 gaaaaatgaa tcgtactcga ggcaactctc actttttcac accaaattaa agttttgatc
    32401 gcaattactt gaaataagcg taatggttac tatagtaaac attaccacaa ggatggatgt
    32461 atgggtgtgt ataaggcaag gcgattcagc caaagcacga aaaaacttgg tattcacgat
    32521 aaagttctca tggctgccgc tgaagaagtg atgcaaggca tatgggaggc tgatttaggt
    32581 agcggtgtca tcaaaaaacg gttacccctc caacaaggaa agagtggtgg agccagaaca
    32641 attatctttt ttaaatcagc aaatcatgtg tttttttatg atggttggtc caagagtggt
    32701 ctaagcagta aaggaaccaa agaaatcgaa gatgatgaac tggcagccta caaaaaaatg
    32761 gcaaatgcat ttctggcctt tagcaataag caaatagagg atttgatcga aactggcttc
    32821 ttgattgagg tgaaaaatga gcgataaatt acaaatgatg ctggaagatg caaaggaact
    32881 gaatgcattg ggcctgatgt ctgatagtga ggttgatgcc attgccgtca tggtcaaagc
    32941 ccgtgaagtc agcgcccgta tagccaaagt caaatcaatg acgggtgaag agataaaagc
    33001 catccgcacc cgatacggca tgtcacaatc cgtgctggca catacaatgg gaatgtcaaa
    33061 agaaagtgtc tcgaaatggg aacgtaatga aataaaaccg agcggcccgg cactacgtat
    33121 tttgaataca ttggcaataa aagggccgga agtttttgca aagtaaaatg tttgcaaatg
    33181 atttaccaca ttcattagta ccccttgatt caggggtact aacactattc taattctact
    33241 gccagctaat ctgagccctg ccgtttttct tactcttata cagtcgctca tccgccagca
    33301 ttttcaagcg ttcgacggta gtacattcat ttgaatgcgc tacaccgata ctggtgctta
    33361 aattaatgct tcccacctgg aaatgaacga tttcttttag tatcgatagc aactcttcca
    33421 gcgtgccgcc agtataaatc accgcaaatt cgtcaccgcc gaagcggtag actttacctt
    33481 tatctaccgg gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca
    33541 gcaaatgacc gaaggtgtcg ttaatatttt tgaatttatc gacgtcaaac aggcacaaat
    33601 aaccgccttt ttccggcagc gcttttaaat cttcgtcaaa ggattttctg cccattgctt
    33661 tggtcaggtc gtccaggaac gccgagttat acatactgta gagttttttg acatcggcag
    33721 ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg gttattaata atggcttctt
    33781 tctccacaat accacgatta atcactctgc gtaccagata gaggcaactg gcacccgata
    33841 tcactgccac caatgcggca aagaaaatga tcaatgtaca aacccgcaaa taaacatctt
    33901 ttttatacgc aaatacgtac aatccggtcg gcaaataggc
//
LOCUS       DACAPB010000028        33745 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.028, whole genome shotgun sequence.
ACCESSION   DACAPB010000028 DACAPB010000000
VERSION     DACAPB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.028"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..273)
                     /locus_tag="IT904_001216"
     CDS             complement(<1..273)
                     /locus_tag="IT904_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAP3191028.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGS"
     gene            complement(273..923)
                     /gene="fabR"
                     /locus_tag="IT904_001217"
     CDS             complement(273..923)
                     /gene="fabR"
                     /locus_tag="IT904_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAP3191029.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            1254..2654
                     /gene="sthA"
                     /locus_tag="IT904_001218"
     CDS             1254..2654
                     /gene="sthA"
                     /locus_tag="IT904_001218"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAP3191030.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(2637..3554)
                     /gene="oxyR"
                     /locus_tag="IT904_001219"
     CDS             complement(2637..3554)
                     /gene="oxyR"
                     /locus_tag="IT904_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAP3191031.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3821..5194)
                     /gene="argH"
                     /locus_tag="IT904_001220"
     CDS             complement(3821..5194)
                     /gene="argH"
                     /locus_tag="IT904_001220"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAP3191032.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSKVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(5255..6031)
                     /gene="argB"
                     /locus_tag="IT904_001221"
     CDS             complement(5255..6031)
                     /gene="argB"
                     /locus_tag="IT904_001221"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAP3191033.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(6039..7043)
                     /gene="argC"
                     /locus_tag="IT904_001222"
     CDS             complement(6039..7043)
                     /gene="argC"
                     /locus_tag="IT904_001222"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAP3191034.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            7197..8348
                     /gene="argE"
                     /locus_tag="IT904_001223"
     CDS             7197..8348
                     /gene="argE"
                     /locus_tag="IT904_001223"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAP3191035.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            8700..11351
                     /gene="ppc"
                     /locus_tag="IT904_001224"
     CDS             8700..11351
                     /gene="ppc"
                     /locus_tag="IT904_001224"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAP3191036.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            11533..13266
                     /gene="cptA"
                     /locus_tag="IT904_001225"
     CDS             11533..13266
                     /gene="cptA"
                     /locus_tag="IT904_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAP3191037.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            13481..14332
                     /locus_tag="IT904_001226"
     CDS             13481..14332
                     /locus_tag="IT904_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3191038.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14319..14660)
                     /locus_tag="IT904_001227"
     CDS             complement(14319..14660)
                     /locus_tag="IT904_001227"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP3191039.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(14662..15540)
                     /locus_tag="IT904_001228"
     CDS             complement(14662..15540)
                     /locus_tag="IT904_001228"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAP3191040.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(15506..17803)
                     /locus_tag="IT904_001229"
     CDS             complement(15506..17803)
                     /locus_tag="IT904_001229"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP3191041.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTVSHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(17854..18174)
                     /locus_tag="IT904_001230"
     CDS             complement(17854..18174)
                     /locus_tag="IT904_001230"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP3191042.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(18189..19269)
                     /locus_tag="IT904_001231"
                     /pseudo
     CDS             complement(18189..19269)
                     /locus_tag="IT904_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
     gene            19578..22079
                     /gene="ptsP"
                     /locus_tag="IT904_001232"
     CDS             19578..22079
                     /gene="ptsP"
                     /locus_tag="IT904_001232"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP3191043.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            22091..22753
                     /gene="fsa"
                     /locus_tag="IT904_001233"
     CDS             22091..22753
                     /gene="fsa"
                     /locus_tag="IT904_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP3191044.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKLLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            22764..23867
                     /gene="gldA"
                     /locus_tag="IT904_001234"
     CDS             22764..23867
                     /gene="gldA"
                     /locus_tag="IT904_001234"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAP3191045.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            24142..24759
                     /locus_tag="IT904_001235"
     CDS             24142..24759
                     /locus_tag="IT904_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAP3191046.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFNWRMVGHYRYFVK"
     gene            complement(24786..25691)
                     /gene="yijE"
                     /locus_tag="IT904_001236"
     CDS             complement(24786..25691)
                     /gene="yijE"
                     /locus_tag="IT904_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAP3191047.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(25784..27964)
                     /gene="katG"
                     /locus_tag="IT904_001237"
     CDS             complement(25784..27964)
                     /gene="katG"
                     /locus_tag="IT904_001237"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAP3191048.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(28293..29183)
                     /gene="metF"
                     /locus_tag="IT904_001238"
     CDS             complement(28293..29183)
                     /gene="metF"
                     /locus_tag="IT904_001238"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAP3191049.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(29532..31964)
                     /locus_tag="IT904_001239"
     CDS             complement(29532..31964)
                     /locus_tag="IT904_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAP3191050.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(31967..33127)
                     /gene="metB"
                     /locus_tag="IT904_001240"
     CDS             complement(31967..33127)
                     /gene="metB"
                     /locus_tag="IT904_001240"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP3191051.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            33404..33721
                     /gene="metJ"
                     /locus_tag="IT904_001241"
     CDS             33404..33721
                     /gene="metJ"
                     /locus_tag="IT904_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAP3191052.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
BASE COUNT         8159 a         9429 c         8532 g         7625 t
ORIGIN      
        1 agagccgata tccggatatt ccgcacgaac taccgtgctg tacagcagta cgccgagaat
       61 aaaacaggca gctgccagac ccggcaagcc taccggcata gtgcgattaa gataacgttg
      121 atgcaggcag taaaccgtca gcaccaggga aataatcggg aatacagaaa atggcacaat
      181 ggagctaaac agcgctgcga aagtaccatt aatcgataag ccagcaacta acgccagcag
      241 cagcgtacct ctatcttgat ttgcttgttt cattactcgt ccttcacatt tcccggaata
      301 attgcggttt tctcttgttc acggcgatac cagtaataag cccctttcga aatcattcgc
      361 agttgcagta ccagtcgctc ttctaattgc cgacgttgtt cgacgccgac gtccaacgcc
      421 tcggcacccg cactgaagac aattgtcacc attgcttcgg cttgcgcttc agtaaacgca
      481 cgcggcatat ggttttcgag ttccagatag tccgcaagtt ccgcaatgaa gtgctgaatt
      541 tcacgcgcaa cggcggcacg aaacgcagcg gaggtgccgg agcgttcccg caataataac
      601 cggaaggcgt taggattatt accgatgaac tccataaatg tggagaccga ggtgcggatc
      661 acactcccgc ctttggcgat acgctgacgc gcctggcgca tgagttggcg tagcattaaa
      721 ccgctctcat caaccatggt cagacccagt tcgtctacgt cgcggaaatg ccgataaaaa
      781 gaggtgggag caatgcccgc ttcacgcgcc acttcacgca aactcaggct ggcgaagctg
      841 cgttcagcac ttaattggct aaatgcggct tccaccagcg aacggcgggt tttttctttt
      901 tgttgcgctc ttacgcccat cacgatgtct gaatccttgc caaaagtact gctggcacta
      961 taccagagaa tgaacatact ggattgctcc agtattgtga atgaacggta acgcaataaa
     1021 acatatttac tgcaattctt tggccggttc ttttacgtac agcggaaacc tgccgcttaa
     1081 acggagagta tcgtcgataa aaatccaata aaacgtcagg gcaaaagtaa gaaacagaca
     1141 aagcaaaggc cgctcaggat atagccagat aaatgacggg gatcaattgg cttacccgcg
     1201 ataaaatgtt accattctgt tgcttttatg tataagaaca ggtaagccct accatgccac
     1261 attcctacga ttacgatgcc atagtaatag gttccggccc cggcggcgaa ggcgctgcaa
     1321 tgggcctggt taagcaaggt gcgcgcgtcg cagttatcga gcgttatcaa aatgttggcg
     1381 gcggttgcac ccactggggc accatcccgt cgaaagctct ccgtcacgcc gtcagccgca
     1441 ttatagaatt caatcaaaac ccactataca gcgaccattc ccgactgctc cgctcttctt
     1501 ttgccgatat ccttaaccat gccgataacg tgattaatca acaaacgcgc atgcgtcagg
     1561 gattttacga acgtaatcac tgtgaaatat tgcaggggaa cgctcgcttt gttgacgagc
     1621 atacgttggc gctggattgc ccggacggca gcgttgaaac actaaccgct gaaaaattta
     1681 ttattgcctg cggctctcgt ccatatcatc caacagatgt tgatttcacc catccacgca
     1741 tttacgacag cgactcaatt cttagcatgc accacgaacc gcgccatgta cttatctatg
     1801 gtgctggagt gatcggctgt gaatatgcgt cgatcttccg cggtatggat gtaaaagtgg
     1861 atctgatcaa cacccgcgat cggctgctgg catttctcga tcaagagatg tcagattctc
     1921 tctcctatca cttctggaac agtggcgtag tgattcgcca caacgaagag tacgagaaga
     1981 tcgaaggctg tgacgacggt gtgatcatgc atctgaagtc gggtaaaaaa ctgaaagctg
     2041 actgcctgct ctatgccaac ggtcgcaccg gtaatactga ttcgctggcg ttacagaaca
     2101 ttgggctgga aactgacagt cgcggacagc tgaaggtcaa cagcatgtat cagaccgcac
     2161 agccgcacgt ttacgcggtg ggcgacgtga ttggttatcc gagcctggcg tcggcggcct
     2221 atgaccaggg gcgcattgcc gcgcaggcgc tggtgaaagg tgaagccacc gcacatctga
     2281 ttgaagatat ccctaccggc atttacacca tcccggaaat cagctctgtg ggcaaaaccg
     2341 aacagcagct gaccgcgatg aaagtgccat atgaagtggg ccgcgcccag ttcaaacatc
     2401 tggcacgtgc acaaatcgtc ggcatgaacg tgggcacgct gaaaattttg ttccatcggg
     2461 aaacaaaaga gattctgggc attcactgct ttggcgagcg cgctgccgaa attattcata
     2521 tcggtcaggc gattatggaa cagaaaggtg gcggcaacac tattgagtac ttcgtcaaca
     2581 ccacctttaa ctacccgacg atggcggaag cctatcgggt agctgcgcta aatggcttaa
     2641 accgcctgtt ttaacacttt atcgaaatgg ccatccattc ttgcgcggat ggcctctgcc
     2701 agctgctcat agcggctgcg cagcggtgag ccaggacgat aaaccaggcc aatcgtgcgg
     2761 cgaggttccg gcttaataca cggcaaataa acaaccccat cgcgtttgcg ctccggcggc
     2821 acagccagcg caggcagtaa agtgatcccg ctacctgccg ccaccatgtt acgcagtgtc
     2881 tccaggctgg tcgcgcggaa gtgtgtatct tcatccgccc cggcttcaaa gcagaaaccc
     2941 attgcctgat cgcgcaaaca gtgaccatct tccagcatca gcagtttttc ccctgccaga
     3001 tcggccatcg gtacgcattc gcggttcgcc cacgggtgat cttcatagat agccagcaac
     3061 attggctcat caaacaacgg cacttcaatg aatgcttcgc tctctttcac cagcgcgagg
     3121 atcacgcaat cgagtttgcc actgtccagt tgcgccagta actggtgggt ctgtgcttca
     3181 tgcagataca tttccagctt tggaaaggtc tggtgcagca tcgggataat atgcggtagc
     3241 aggtacggtc caaccgtggg aatcaaacca atatgcagcg gtccggacat cgtctcgccc
     3301 tgctggcttg ccatctcttt aaggactttc acctcacgca gcacggtacg cgcctgatcc
     3361 accagcagca ttcccgcctg ggtgaacaac actttacggc tggtccgctc cagcaacatc
     3421 acgcccaact catcttccag cttacgaatt tgcccgctaa gcgtcggctg gctaacgtgg
     3481 caggaatctg ccgcacgccg aaaatggcgg tgttcagcca atgccaccag gtactcaaga
     3541 tcacgaatat tcattatcca tcctccatcg ccacgatagt tcatggcgat aggtagaata
     3601 gcaatgaacg attatcccta tcaagcattc tgactgataa ttgctcacag aaacggagcg
     3661 gcacctcttt taacccttga agtcactgcc cgtttcgaga gtttctcaac tcgaataact
     3721 aaagccaacg tgaacttttg cggatctcca ggatccgctt ttttttgcca taaaaaagcc
     3781 cggcgataag ccaggctcaa atttgtacat ataaatgttc ttaccccaac cgagcctgcg
     3841 caaaagcaat cgcctgcgcc acctgctgcg gtgagacgcc gccttttgcc gcacgcttgt
     3901 cgaggcacga ttgcagcgac agaatcggat agacatcttc gtcaatcacc ttactgaatt
     3961 tctgcaactc actgagcggc agatcttcca gcggtttgcc ctgacgaatg gcttccacca
     4021 ccgcttcgcc aacaatatgg tgcgcctcgc ggaacggtac gcctttcgcc accagataat
     4081 ccgccagttc ggtggcgttg gcgtaaccct gctgagccgc ttcctggcaa cgtggacgtt
     4141 tcacctgaat gccgtccagc accagcgccg ccatatgcag gcagtccagc caggtatcga
     4201 gcgcgtcgaa cagaccttct ttgtcttcct gcatatcttt gttgtaagcc agcggcaaac
     4261 ctttcagcgt catcatcatg ccggttaacg ccccctgcac ccgaccgcat ttaccgcgaa
     4321 tcagctccag cgcatccggg tttttcttct gcggcattaa tgatgaaccg gaagtcacgc
     4381 ggtcagaaag ctccacaaac cccgcttcgc cggtgttaaa gaaaatcaga tcttcagcaa
     4441 aacgcgacag atgcaccatg ccgatagcgg cagcagaaag cagttccaac acatggtcac
     4501 ggtcagaaac gctgtcgaga ctgttacggg tcgccgaagc aaagcccagc cagcctgcta
     4561 actgttcacg gtcgatttca taggccgttc ccgccagcgc gccacagcct agcgggctga
     4621 catccagacg cttaagcgca tcctgcaaac ggctttcatc acgcgccagc atctcaacat
     4681 aggccaggca ccagtgcgcg aacgtcaccg gctgggcgcg ttgcaggtga gtgtaacctg
     4741 gcattaccgc gtcctgattg ttttgtgcgg tttccaccag cgccgattgc agctgccggt
     4801 tagccgtcag taactcgcta acggtatctt tgcaccacag tttcaggtca gtcgctacct
     4861 gatcattacg gctacgcccg gtatgcagct ttttgcctaa ctggcccact ttgtcgatca
     4921 gtttgccttc cacccagcta tggatatctt cggcgtcgct ttcaaggatt tgttgtggcc
     4981 tggcgcgaac atcttccagc aacacgttca gcgcctcttc cagttgcgcc tgctcttctg
     5041 cggttaacac gcctaccgtg accagggctt tggaccaggc cacagagcca acaatatcct
     5101 gctccgccag acggtaatca aagcgcagtg agtcgttgaa ttgtttgaac cgttgatctg
     5161 ctgcctgggt aaaacgcccg ccccaaagtg ccataactct gtttccttat tttgaaattc
     5221 aatgccggaa agctgcgcct ccggccaaca aaacttaagc taaaatccgc gtacccatcg
     5281 gcataccgtt aaacagtgcc ggaagctgct ccgcatgacg ccaggaggcg atatctaccg
     5341 gacggcccag cgtgcgggcc gcatccagcg ccgcgttcac tttcactatc atgccgtcag
     5401 taataatgcc ctgctcaatc agttgttctg ctttcgcggc ggtcatttcg gcaatgcgtt
     5461 gccctttgcc gtcgagaatg ccgctgacgt cggagagcaa aatcagatcc gcgcccagcg
     5521 ttgccgccag cgccgttgcc gcctggtcgg cattgacgtt catcagttgc ccttcgtctg
     5581 ttacgccaat ggagctgacc accggcagat aaccgttctc cagcaaggag ttgataagct
     5641 taggcgaacc tggctgcgcc agtccaacat gacctaactc ttcatcaagc tgggtcactt
     5701 tgacgctgtc gccgtcaccg agaaacaaac ctacggccgc aatctgatgt ttcttcgccc
     5761 atgccaacag ggttttattt gccgttcccg ccagtgctcc ggtgataatg tctatctgat
     5821 cagcaggcgt cacccgcagg ccgtttttct ttttcaccgg cagattcagc cctttcatca
     5881 gctcatccac cacgcaaccg ccgccgtgca caatcaccag cggacgctga tgtgactcac
     5941 gataattcac cagtgcgcta aacagacgtt ccagcgcctc ttcactatcc agcagtacgc
     6001 cgcccagttt gataattaat ggattcatca ttgcaccctt aaataagaga ctgcgtttcc
     6061 gcatagccga aacgaatatt ggcgcactgt accgcttgtg ccgccgcgcc tttcagtaag
     6121 ttgtcttcgg tcgccacaat aatcaaatgc tcgccctgaa cggcaaaccc gatatcgcaa
     6181 aatggcagcc caacgacatt tttcagcgcc ggaacgcctt tgtcatacag ccgcaccagc
     6241 ggtttatggg catacgcctg ctgtaacact tgcgcgactt gcgcctgggt cacacccgat
     6301 ttcaggcggc aggtaatggt ttcgagaatg ccgcgcggga aattgcccag atgtggggtg
     6361 aagataacgt cagcaccgag gtgtgtggcg atctctggtt gatggcgatg agtaaagacg
     6421 ccatacggtt gcaggctaac ttcacaaaag ctgtttgaaa tggccgcttt acgccctgca
     6481 ccgctcacgc cgctggtggc gttgatcacc ggccactgat tgaggtcaag aagatcggca
     6541 tcaatcaacg gtttcagcgc cagctgtgcc gccgtcggat aacagcccgg caccgcaatc
     6601 aaattcgctt cttttaattt attaccgcac cactccgcca gaccgtaggc tgcctgttcc
     6661 aacagttccg ggtattgatg ggtaaagccg taatattttt catagaaggt ggcgtcgtta
     6721 acacgaaacg cgccggaaag gtcgaacacc acgcagcccg cttcaagaaa ctgcggcgct
     6781 aaatcgtggc taacttcatg ggcggtggcg agaaacacta cgtccacccc tgggctaaac
     6841 tcgctgatat ccgacatcgg ctgcaacggc agatcaacga tgccttttag ctgcggatgc
     6901 aaatcggaga ttaactttcc cgcatcattg ctttgcgctg aaacagtcaa agcggttatg
     6961 ttcatatgcg gatggcgatt tacataggtc actagctctg cgccagcgta gccgctggca
     7021 cccacaatca gcgtattcaa catcggggct attcaccttc ttatgtctgg ttgccaggtt
     7081 aaacgtaaaa cattcacctt acggctggtg ggttttatta cgctcaacgt tagtgtattt
     7141 ttattcataa atactgcatg aatattgata ctatcatgac cagaggtgtg tcaacaatga
     7201 aaaacaaatt accgccattt atcgagattt accgcgctct gattgccaca ccttcaataa
     7261 gcgccacgga agaggcactc gatcaaagca atgcagattt aatcactctg ctggcggact
     7321 ggtttaaaga tttgggcttc aatgtggaag tgcagcctgt tccaggaact cgcaacaaat
     7381 tcaatatgct ggcaagtatc ggacaggggg ctggcggctt gttgctggcg gggcataccg
     7441 atacggtgcc atttgatgac ggtcgctgga cgcgcgatcc gtttacactg acggagcatg
     7501 acggcaagct ttacggctta ggcaccgccg acatgaaagg cttttttgcg tttatccttg
     7561 atgcgctacg cgatgtcgac gtcacgaaac tgaaaaaacc gctctacatt ctggcgactg
     7621 ctgatgaaga aaccagtatg gccggagcgc gttattttgc cgaaactacc gccctgcgcc
     7681 cggattgcgc catcattggc gaaccgacgt cactacaacc ggtacgcgca cataaaggtc
     7741 atatctctaa cgccatccgt attcagggcc agtcggggca ctccagcgat ccagcacgcg
     7801 gagttaacgc tatcgaacta atgcacgacg ccatcgggca tattttgcaa ttgcgcgata
     7861 acctgaaaga acgttatcac tacgaagcgt ttaccgtgcc ataccctacg ctcaacctcg
     7921 ggcatattca cggtggcgac gcttctaacc gtatttgcgc ttgctgtgag ttgcatatgg
     7981 atattcgtcc gctgcctggc atgacactca atgaacttaa tggtttgctc aacgatgcat
     8041 tggctccggt gagcgaacgc tggccgggtc gtctgacggt cgacgagctg catccgccga
     8101 tccctggcta tgaatgccca ccgaatcatc aactggttga agtggttgag aaattgctcg
     8161 gagcaaaaac cgaagtggtg aattactgta ccgaagcgcc gtttattcaa acgttatgcc
     8221 ctacgctggt gctcggtccg ggttccatca atcaagctca tcagcctgat gaatatctgg
     8281 agacgcggtt tattaaaccc acccgtgagt tgattatcca ggtaattcac catttttgct
     8341 ggcattaaaa cgtaggccgg ataaggcgct cgcgccgcat ccggcaccgt tgccaaactc
     8401 ctgtgccgca ataatgtcgg atgcgatgct tgcgcatctt atccgaccta cacctttggt
     8461 gttatgtatg gcgatttttt aacatttcca taagttacgc ttatttaaag cgtcgtgaat
     8521 ttaatggcgt aaattcctgc tatttattcg tttgctgaag cgatttcgca gcatttgacg
     8581 tcaccgcttt tacgtggctt tataaaagac gacgaaaagc aaagcccgag catattcgcg
     8641 ccaatgcgac gtgaaggata cagggctatc aaacgataag atggggtgtc tggggtaata
     8701 tgaacgaaca atattccgca ttgcgtagta atgtcagtat gctcggcaaa gtgctgggag
     8761 aaaccatcaa ggatgcgttg ggagaacaca ttcttgaacg cgtagaaact atccgtaagt
     8821 tgtcgaaatc ttcacgcgct ggcaatgatg ctaaccgcca ggagttgctc accaccttac
     8881 aaaatttgtc gaacgacgag ctgctgcccg ttgcgcgtgc gtttagtcag ttcctgaacc
     8941 tggccaacac cgccgagcaa taccacagca tttcgccgaa aggcgaagct gccagcaacc
     9001 cggaagtgat cgcccgcacc ctgcgtaaac tgaaaaacca gccggaactg agcgaagaca
     9061 ccatcaaaaa agcagtggaa tcgctgtcgc tggaactggt cctcacggct cacccaaccg
     9121 aaattacccg tcgtacactg atccacaaaa tggtggaagt gaacgcctgt ttaaaacagc
     9181 tcgataacaa agatatcgct gactacgaac acaaccagct gatgcgtcgc ctgcgccagt
     9241 tgatcgccca gtcatggcat accgatgaaa tccgtaagct gcgtccaagc ccggtagatg
     9301 aagccaaatg gggctttgcc gtagtggaaa acagcctgtg gcaaggcgta ccaaattacc
     9361 tgcgcgaact gaacgaacaa ctggaagaga acctcggcta caaactgccc gtcgaatttg
     9421 ttccggtccg ttttacttcg tggatgggcg gcgaccgcga cggcaacccg aacgtcactg
     9481 ccgatatcac ccgccacgtc ctgctactca gccgctggaa agccaccgat ttgttcctga
     9541 aagatattca ggtgctggtt tctgaactgt cgatggttga agcgacccct gaactgctgg
     9601 cgctggttgg cgaagaaggt gccgcagaac cgtatcgcta tctgatgaaa aacctgcgtt
     9661 ctcgcctgat ggcgacacag gcatggctgg aagcgcgcct gaaaggcgaa gaactgccaa
     9721 aaccagaagg cctgctgaca caaaacgaag aactgtggga accgctctac gcttgctacc
     9781 agtcacttca ggcgtgtggc atgggtatta tcgccaacgg cgatctgctc gacaccctgc
     9841 gccgcgtgaa atgtttcggc gtaccgctgg tccgtattga tatccgtcag gagagcacgc
     9901 gtcataccga agcgctgggc gagctgaccc gctacctcgg tatcggcgac tacgaaagct
     9961 ggtcagaggc cgacaaacag gcgttcctga tccgcgaact gaactccaaa cgtccgcttc
    10021 tgccgcgcaa ctggcaacca agcgccgaaa cgcgcgaagt gctcgatacc tgccaggtga
    10081 ttgccgaagc accgcaaggc tccattgccg cctacgtgat ctcgatggcg aaaacgccgt
    10141 ccgacgtact ggctgtccac ctgctgctga aagaagcggg tatcgggttt gcgatgccgg
    10201 ttgctccgct gtttgaaacc ctcgatgatc tgaacaacgc caacgatgtc atgacccagc
    10261 tgctcaatat tgactggtat cgtggcctga ttcagggcaa acagatggtg atgattggct
    10321 attccgactc agcaaaagat gcgggagtga tggcagcttc ctgggcgcaa tatcaggcac
    10381 aggatgcatt aatcaaaacc tgcgaaaaag cgggtattga gctgacgttg ttccacggtc
    10441 gcggcggttc cattggtcgc ggcggcgcac ctgctcatgc ggcgctgctg tcacaaccgc
    10501 caggaagcct gaaaggtggc ctgcgcgtga ctgaacaggg cgagatgatc cgctttaaat
    10561 atggtctgcc agaaatcacc gtcagcagcc tgtcgctgta caccggggcg attctggaag
    10621 ccaacctgct gccaccacct gagccgaaag agagctggcg tcgcattatg gatgaactgt
    10681 cagtcatctc ctgcgatctc taccgtggct acgtacgtga aaacaaagat tttgtgcctt
    10741 acttccgctc cgctacgccg gaacaagaat tgggcaaact gccgttgggt tcacgtccgg
    10801 cgaaacgtcg cccaaccggc ggcgtcgagt cactacgcgc cattccgtgg atcttcgcct
    10861 ggacgcaaaa ccgtctgatg ctccccgcct ggctgggtgc aggtacggcg ctgcaaaaag
    10921 tggtcgaaga cggcaaacag agcgagctgg aggctatgtg ccgcgattgg ccattcttct
    10981 cgacgcgtct cggcatgctg gagatggtct tcgccaaagc agacctgtgg ctggcggaat
    11041 actatgacca acgcctggta gacaaagcac tgtggccgtt aggtaaagag ttacgcaacc
    11101 tgcaagaaga agacatcaaa gtggtgctgg cgattgccaa cgattcccat ctgatggccg
    11161 atctgccgtg gattgcagag tctattcagc tacggaatat ttacaccgac ccgctgaacg
    11221 tattgcaggc cgagttgctg caccgctccc gccaggcaga aaaagaaggc caggaaccgg
    11281 atcctcgcgt cgaacaagcg ttaatggtca ctattgccgg gattgcggca ggtatgcgta
    11341 ataccggcta atcttcctct tctgcaaacc ctcgtgcttt tgcgcgaggg ttttctgaaa
    11401 tacttctgtt ctaacaccct cgttttcaat atatttctgt ctgcatttta ttcaaattct
    11461 gaatatacct tcagatatcc ttaaggaatt gtcgttacat tcggcgatat tttttcaaga
    11521 caggttctta ctatgcattc cacagaagtc caggctaaac ctctttttag ctggaaagcc
    11581 ctgggttggg cactgctcta cttttggttt ttctctactc tgctacaggc cattatttac
    11641 atcagtggtt atagtggcac taacggcatt cgcgactcgc tgttattcag ttcgctgtgg
    11701 ttgatcccgg tattcctctt tccgaagcgg attaaaatta ttgccgcagt aatcggcgtg
    11761 gtgctatggg cggcctctct ggcggcgctg tgctactacg tcatctacgg tcaggagttc
    11821 tcgcagagcg ttctgtttgt gatgttcgaa accaacacca acgaagccag cgagtattta
    11881 agccagtatt tcagcctgaa aattgtgctt atcgcgctgg cctatacggc ggtggcagtt
    11941 ctgctgtgga cacgcctgcg cccggtctat attccaaagc cgtggcgtta tgttgtctct
    12001 tttgccctgc tttatggctt gattctgcat ccgatcgcca tgaatacgtt tatcaaaaac
    12061 aagccgtttg agaaaacgtt ggataacctg gcctcgcgta tggagcctgc cgcaccgtgg
    12121 caattcctga ccggctatta tcagtatcgt cagcaactaa actcgctaac aaagctactg
    12181 aatgaaaata atgccttgcc gccgctggct aatttcaaag atgaatcggg taacgaaccg
    12241 cgcaccttag tgctggtgat tggcgagtca acccagcgtg gacgcatgag tctgtacggt
    12301 tatccgcgtg aaaccacgcc ggagctggat gcgctgcata aaaccgatcc gaatctgacc
    12361 gtgtttaata acgtggttac gtctcgtccg tacaccattg aaatcctgca acaggcgctg
    12421 acctttgcca atgaaaagaa cccggacctg tatctgacgc agccgtcgct gatgaacatg
    12481 atgaaacagg cgggttataa aaccttctgg atcaccaacc agcagacgat gaccgcccgc
    12541 aataccatgc tgacggtatt ttcgcgccag accgacaagc agtactacat gaaccagcaa
    12601 cgtacgcaga gtgcgcgtga atacgacacc aacgtgctga agccgttcca ggatgtgctg
    12661 aatgaccctg cgccgaagaa actgatcatc gttcatctgc tgggtacgca tatcaaatac
    12721 aaataccgct acccggaaaa tcagggcaag tttgatggca ataccgatca tgttccgcca
    12781 ggattaagcg cagaagagct ggaatcatat aacgattatg acaacgctaa cctgtataac
    12841 gatcatgtgg ttgccagcct gattaaagac tttaaagcgg cagacccgaa cggattcctg
    12901 gtttacttct ctgaccacgg tgaagaggtt tacgacacgc cgcctcataa aacccagggg
    12961 cgtaatgagg acaacccgac gcgtcatatg tacaccattc cgttcctgct gtggacgtcg
    13021 gaaaaatggc aagcgactca tccccgtgat ttctcacagg atgtcgatcg taaatacagc
    13081 ctggcggaac tgatccacac ctggtcagat ttggcgggct tatcttacga cggttacgat
    13141 ccaacccgtt cagtggtgaa tccgcagttc aaagaaacta cccgctggat tggtaacccg
    13201 tacaagaaaa acgcgctgat cgattacgac acactgccgt atggcgatca ggtgggtaat
    13261 cagtaattat cagccaggcg ctatttgccg gatgcgacgc ttgacgcgtc ttatccggcc
    13321 tacagatcgc ggcggtaggc tggataaggc gtttacgcca catccggcaa tacaactgcc
    13381 gcaatgttta catcgaactc ccctcaggcc acacttttca ggtggccttt tttaataatg
    13441 ccagttactg ttttcccgtc tacataccta agggaataac atgtatcacg acgtcagcta
    13501 tctgctctcc cgcctgatca acggcccgct gtccctgcgc cagatttact ttgccagcag
    13561 taacggcccg gtgcccgatc ttgcgtatca ggtcgatttt ccacggctgg aaattgtgct
    13621 ggaaggtgag tttgttgata ccggcgctgg agcaacgtta gttcccggcg atgtgctgta
    13681 cgttgccgct ggtggctgga atttcccaca atggaaaaca cccgctacca cctttagcgt
    13741 gctgtttggc aaacagcaac tcggcttcag cgtcgtgcaa tgggatggca aacaatatca
    13801 aaatctggcg aagcaacacg tcgcccggcg cggcccacgc ataggttctt ttctgctaca
    13861 aacgctcaat gaaatgcaaa tgcagccgca ggagcagcaa acggcaaggc ttatcgtcgc
    13921 cagcctgctt agccactgcc gcgatttgct tggcagccaa atccagaccg cctcacgcag
    13981 ccaggcacta tttgaagcta ttcgcgatta tatcgacgaa cgctatgcct ccgcgcttac
    14041 ccgcgaatct gttgcgcagg cgttttatat ttcgccaaat tacctctcgc acctgtttca
    14101 aaaaacgggg gccattggtt ttaacgaata cctgaatcac acgcgactgg agcacgctaa
    14161 gacgttattg aaaggctatg acctgaaggt aaaagaagtg gcgcacgcct gcggctttgt
    14221 cgacagcaac tacttctgcc gtctgtttcg taaaaacacc gaacgctcgc cgtcggaata
    14281 tcgtcggcag taccacagcc aactgacaga aaaaccgact actccagaat aagatgggtt
    14341 tgctgcggcg cagaaagcac tttgcgcacc gcgctcatta cccgctgcgg ctcacgcagg
    14401 aatgcgtaga tgctgcattg cacatagcgg caatgttcaa atcgctcggc accggcaagc
    14461 tcgatatccg taatcaacag agcaacatcc gcccgacgga tatcctcgtc cgctaaacga
    14521 ttctccgttc ccagcgcgcc ctgagtttca atgctgactc cccacttctc taacaggcac
    14581 aacttttcca gccgttccgc cgccatataa gtatgcgcca cgccactgac gcaggcggtt
    14641 actgccacca ggtatgccat tttaacctcc cacggtaacc tgaaatccgg cccgttctgc
    14701 catttcgcgc atcgttgtca catcggctga cgacggcgca ggcacctctt tcatcgacca
    14761 tgttttcccc agcaggcggt atttcggttc gccgtactga tgaaacggta acagatggat
    14821 ctgcctgatt ttcagcggga tcagtacatc cagcgcctgc tgcatattct cccggctgag
    14881 cgtgaaacca gggatcaacg gtaaacgcgg gatcacgttg acaccctcac tcaccagcaa
    14941 acgcagattc tccagcacgc gtggcaggtt catcttcacc acatcccgcg cctgagtcgc
    15001 gtccataatt tttaaatcga acagcacttc atcgcacaat ttcgccagcg gtaacagctt
    15061 gcttgctggc gcgtctccgg cagtttcaat ggcgcatgac acaccccaca gccgcagtcg
    15121 ctgtaaaaaa cgggtagcaa actccgcctg cattaacact tcgccgccag aaagcgtgac
    15181 gccgccgccg gacgtgcgaa aaaaaatgtc atctttcatc acttcccgtt ccagagcgtc
    15241 aaggctgata tcgcgaccaa tccgttcaaa cgccccggag gggcattcat ccgcatcacg
    15301 caaacatttc gcacagtgca gacatttcgc ctctctgcgt accgtctgga ttttgccgga
    15361 gatcgactcc ggattagcac accacgggca aagatgcgga cagcctttaa aaaagaccac
    15421 cgtacgaatg ccctcaccgt cattcagtga ataacgctga atgttgaaaa tgcgcgccac
    15481 atcattgcgg cgcgtttcca caacgttaca gctgatgcgc tgtccggcgg atgatgtcat
    15541 cctggatctc cttcgacagt tcgacaaaga aggcgctgta tccggcaacg cgcaccacca
    15601 gcccggcata atcttgtggg cgctgttgcg cttcccgcaa cgtgtcggcg ttcaccacgt
    15661 taaactgaat atgttgtaac ttaagctggg taaacgcccg taagaagtcg gccagtttgc
    15721 gtaatcccgc ttcaccttcc agggtcgccg gagtgaattt cacgttcagc aatgtaccgt
    15781 tagacagcag tgtgttatcg agcttactga ctgacttcag taccgccgtt ggcccttgtg
    15841 cgtcctgacc cagcataggt gacaagccgc cgtctgccag ctgttctccg gcaaaacgac
    15901 cgtctggcgt cgcgccaacc accgatccca acggaacgtg agcagaaacg gtatacgatc
    15961 ccggcgtgaa gtagccgccg cgcgggttct ggtatttttc cacttctttg cagtagtggc
    16021 gcaacagttc ggcgctaatg ttatccacct cgtcgatatc gttaccgtat ttctcaaagc
    16081 ggttaattaa gcgagcgcgg actttttcgc cttctggcgt tgcaaagttg gcttttaata
    16141 ccgacagcaa ttcgtcaaaa cttaaacgct gttgatcaaa aaccatccct ttcaacgcat
    16201 ggagagaatc gctcaggttg gcgataccga tcccctgtac gccggagaag ttataacgcg
    16261 cgccgccatc ggtaatatcg cggccttttt ccagacaatc gctgataaaa gacgagagca
    16321 gcggtacagg tgcccagtcg cgatggccga tatcgcaaat attactgcct tcaaccatca
    16381 gggtgatgta gtggctgatc ttggcacgga tctgttccag taaaccttca taagtcagcg
    16441 cggcattgcc ttcattttca tgcaggcaga tttccatcac tttcagcagg ttaaacatcg
    16501 cgatgtcatg caagccgtag gttctgccgg gaatagataa ttccacacag cccactacgg
    16561 aatagtcgcg cgcatcttcc agcgaaacgc cacggttgag gaacgctggc accaccactt
    16621 catcgttaaa gatttgcgga ataccggtgc cgaggcgaat ggtttcggcg gttttcatca
    16681 ggaacggcgt gtcgatcaag gcgttagtgc gcacgccgag gttcggttgc ggtaattgca
    16741 cgctttgata ggcgtcaagg caaaggaacg aaagcacgtt caccgcgcta cgtccgttct
    16801 cggttaaccc gccgagcagt gcggtatagc cggtcgggaa accggcgaaa taacgcgcgc
    16861 tgctggtgga gcgcaacagc acgatgtcgt tgcatttcac ccataaagat tcgagcagtt
    16921 ctttcaggaa cgccgcatct tcgccctggg ttaatgatgt ctgatagaac ggcaacatat
    16981 actggtcgaa gcgccccaac gatagcgaac tggcgttgga ttcgtattgc agaatgatgt
    17041 tcatgtacca gaataactgg cacgcctgcc agaacgtctg cggcttatgt tgcgcgttat
    17101 ggcgggagat ctccgcaata gtcagcagct cttcgcgacg ctgggcatct gtgcagtttg
    17161 ccgccatcgt ttccgccagt tcggcgtaac gcaaaatgtg tttctgcgag gcttccagca
    17221 gtaacagcgc tgcctgataa aagtgattct ccggctgttg ctgacaatgt tgctgcatct
    17281 gtgctaccag ctcccccagc ccgtgattca gcaggcgtgg gtaatcaata ataatgtgcc
    17341 cctggccttt atctgtctgg ttgatgctga aaatctgcgt gctggtcgcg gcttttactt
    17401 catctgtcat ctgcccgttg atgaaatctt tcatcgaacg tttttcccag tacgggaaca
    17461 actcttcgcg gtagatacgt ttatcttctt cgctgatagc aaagcggtcc tgcggacgcg
    17521 tcgggaattg atccagctct ttcagcagcc agtaagggtc catttccggc gacataatcc
    17581 cggcgcgcgg ttttacggtg cggttaccgg caatcagttc ttcatcacga atcgaaattt
    17641 caacatgttc aaggatatac gctgttgctt tcgcccggcg caatatcacc ggttcgcctt
    17701 cggtttgccg atggctgaca gtataaagca gcgcccgctc cagcgagatt tcacgggtat
    17761 tggcaaacag tgcagttttg aggcgagaga tacgattcgt catgaggaac tcctgtggga
    17821 aagcgaaccc gcccttacgg gcgggataag aacttaagcg gtttgcgcca ggtgagcttc
    17881 aattttgctc ataatggcat cagcacgttt taccgcatcg ctgatgttga cgcgcacgat
    17941 ggtcttgcca gcaaaacgct cttcaaactt gatgccgata tctttagtca gaatcaccat
    18001 atcggcgctc gccacgtctt ccgcagtcag ttcattttcc agaccaattg agccctgggt
    18061 ttctactttc acttcccagc ctttcgcttt cgcggcactt tccagcgctt ctgctgccat
    18121 ataggtatgg gcaacacctg aaggacatgc ggttactgca ataattttcg tcatggcttg
    18181 ttcctcaatt aattaatttc aaaatccaaa tccaggtcgt cttctttttc atcagtcgaa
    18241 gacccatttt tacgcgccag acttttcagc acgttcacac aaacagccgt caccactgct
    18301 ccgacagcca ctgcggcgat ataacccagc ttgccttcaa ccaccggcag tacaatcaga
    18361 ccaccccagc ctgcatagca ctgcgcaccg accagcgccg cagttaccgc accacaaaca
    18421 gaaccaacca tgatggaagg aatgacacgc agcggatcgg cagcggcgaa aggaatcgcc
    18481 ccttcagtaa ccccaacgca ccccatcacc agcgccgctt taccagtttc gcgctcttct
    18541 gcggagaaat ttttacgacc aatcagcgtc gccagcccca tccccagcgg tggaacacaa
    18601 atcccaacag cggcaatagc caccacggta taaacaccct gagcaacgca aatcagcatg
    18661 aaggcatagg ccactttgtt aaccggaccg cccatatcga acgccagcat cagacccatg
    18721 atcaccgcca gcataacaat gctgccctgc tgcatcccct gaagccactg agtcagactg
    18781 ttggtcaacg cccctacagg ctcgcccaag ccccacatca tgatgcctgc ggtaatcagt
    18841 gtgccgacga taggaatgat gaagataggc atcaccgatg cgcagaactt tatgcaccgg
    18901 aattttcttc aggtaatgca ccacgatgcc gccgataatc ccggcgatca gcgcaccaaa
    18961 gaagcccgca ccaaagctgt taccaaccca ggccccgata gcgcacggag ccagcgcaga
    19021 acgctctgca atggagtaac caatgtaagc ggcgaggaaa ggcaccatca gcgtcaaacc
    19081 cgcaacgccg atatcaaaca gttttttcag attcggatcg gctacggcat ccggcactgc
    19141 gcctttgcca tacaacatga cggaaaccgc cagcaaaata ccgcccgata ccacgaaggg
    19201 aatcatgtgt gaaacgcccg tcattaaatg ctgacgggta tttttcagga tctgcaccaa
    19261 ctcattcata agctgctccc gggcttgcaa aagcccatgt ccatattttt gatggaggaa
    19321 acaataggca atcgcaggcg gagcaaacag tggataaaaa ggccatttac tggacttttg
    19381 taatatcagt acaaaaatac gatccgcctc ataacttgcg ataatccgga agcatgccgc
    19441 aaaacggcgt cttgtgagac gcgtcgcaaa ttgacggtta cattacattt gtccagcttt
    19501 tggcagattt gtcacatggc aggccgttgc cgtgcgctct tatcctgtaa acagaaaacc
    19561 gtaccgggag agaggcaatg gccctgattg tggaatttat ttgtgagcta cctaacggcg
    19621 tacatgcacg tccggcaagc cacgttgaaa cgctgtgtaa tactttttca tcacaaattg
    19681 agtggcataa cctgcgcact gaccgtaaag gcaacgccaa aagcgccctg gcgctgattg
    19741 gcaccgacac gctggtgggc gataattgcc agttactgat ttccggggcc gacgaacagg
    19801 aagcgcacca gcgtttaagc caatggctgc gcgatgaatt cccccactgc gatgcgccgc
    19861 tggcggaagt taaatctgac gaactggaac cactgccggt ttcattgacc aatctgaatc
    19921 cgcaaattat ccgcgcccgc accgtgtgca gcggtagcgc aggcggcatt ctgacgccga
    19981 tctcttcttt agatctcaat gcgctgagta atcttcccgc tgccaaaggc gttgacgccg
    20041 aacaatccgc actggaaaac ggcctgacgc tggtactgaa aaacattgag tttcgtctgc
    20101 tggatagcga cggtgctacc agcgcgattc tggaagcgca ccgatccctg gctggcgata
    20161 cttccctgcg cgaacattta ctggcaggcg tcagcgccgg attaagctgc gccgaagcga
    20221 ttgtcgccag tgcgaatcac ttttgcgaag agttcgcccg ttccagcagc agctacctgc
    20281 aagaacgtgc cctggacgta cgcgacgtct gcttccagtt actccagcaa atctacggtg
    20341 agcaacgctt cccggcaccg ggcaaactga cgcagcccgc catttgtatg gctgatgaac
    20401 tgactcccag ccagttcctc gaactggata aaaatcacct caaaggtctg ttactgaaaa
    20461 gtggcggcac cacctcacat accgtaattc ttgcccgttc gttcaacatt ccgacactgg
    20521 ttggtgtgga tattgatgcc ctcactccgt ggcagcatca aacgatttat atcgacggca
    20581 acgctggggc gattgtggtt gagccagggg aagccgtagc tcgttattat cagcaagaag
    20641 cccgcgtaca ggacgccctt cgtgagcaac agcgtgtctg gctgacccaa caagcccgta
    20701 ccgctgacgg tatccgtatt gaaattgccg ctaacatcgc tcactccgtg gaagcgcagg
    20761 ccgcattcgg caatggtgcg gaaggcgttg gtttgttccg cactgaaatg ctctatatgg
    20821 atcgcaccag tgcaccgggt gaaagcgagc tgtacaacat tttttgtcag gcgctggagt
    20881 ctgccaacgg acgcagcatt attgtgcgca ctatggacat tggcggtgat aaacccgttg
    20941 attatctgaa cattccggca gaagctaacc cgttcctcgg ttatcgcgcg gtgcgtattt
    21001 atgaagaata cgcgtcgttg tttaccacac aactacggtc aatcctgcgc gcctctgctc
    21061 acggcagcct gaaaatcatg atcccgatga tctcctcaat ggaagagatc ttatgggtga
    21121 aagaaaaact ggcggaagcc aaacagcaac tgcgtaacga acacattccg tttgatgaga
    21181 agatccagct cggcattatg ctggaagtgc cgtcggtgat gttcatcatc gatcaatgct
    21241 gcgaagagat tgatttcttt agtattggta gtaatgacct gacgcaatat ctgctggcgg
    21301 tggatcgcga taacgctaag gttactcgtc actacaacag cctgaatccg gcattcttgc
    21361 gggcgctcga ttacgccgtg caggcggtgc atcgccaggg caaatggatt ggtctgtgcg
    21421 gtgagctggg agcgaaaggt tccgtgctgc cgttgctggt cggtttaggg ctggatgaac
    21481 tcagcatgag cgcaccgtca attccggcgg cgaaagcgcg aatggcgcaa cttgatagcc
    21541 gtgagtgccg ccagttgctc aaccaggcaa tggcctgccg tacttcgctg gaagtggaac
    21601 atctgctggc gcaattccgc atgacccaac aggacgcacc gctggtcacc gccgagtgca
    21661 tcacgctgga aagtgactgg cgcagcaaag aagaagtgct caaaggcatg accgataacc
    21721 tgctgctggc gggccgctgc cgctatccgc gtaaactgga agccgactta tgggcgcgcg
    21781 aggccgtttt ctctaccggt ctgggcttta gttttgccat tccccacagc aaatcagaac
    21841 acattgagca atccaccatc agcgtagcgc gtctgcaagc gccggtgcgc tggggcgatg
    21901 atgaagcgca attcatcatt atgttaaccc tgaacaaaca cgctgcgggc gatcagcata
    21961 tgcgcatttt ctcgcgcctc gctcgccgca tcatgcacga agaattccgt aacgcgctgg
    22021 ttaacgccgc ctctgccgac gctatcgcca gcctgctgca acatgaactg gaactgtaaa
    22081 aggaaacatc atggaactgt atctggacac cgctaacgtc gcagaagtcg aacgtctggc
    22141 acgcatattc cccattgccg gggtgacaac taacccgagc attatcgctg ccagcaagga
    22201 gtccatctgg gaagtgctgc cgcgtctgca aaaagcgatt ggtgatgagg gcattctgtt
    22261 tgctcagact atgagtcgtg acgcgcaggg tatggtggaa gaagcgaagc gcctgcgcga
    22321 cgccattccg ggcattgtgg tgaaaatccc ggtgacttcc gaaggtctgg ccgccattaa
    22381 gttgctgaaa aaagagggta ttactacact tggcaccgct gtatatagcg ccgcacaagg
    22441 gttattagcc gcactggcag gggcaaaata cgttgctccg tatgttaacc gcgtagatgc
    22501 ccagggcggg gacggcattc gtacggttca ggagctgcaa acgctgttag aaatgcacgc
    22561 gccagaaagc atggtgctgg cggccagctt taaaacgccg cgtcaggcgc tggactgttt
    22621 actggcagga tgtgaatcca tcaccctgcc cttagatgta gcgcaacaaa tgctcaacac
    22681 ccctgcggta gagtcagcta tagagaagtt cgaacacgac tggaatgccg catttggcac
    22741 tactcatctc taaaggagca attatggacc gcattattca atcaccgggt aaatacatcc
    22801 agggcgctga tgtgattaat cgtctgggcg aatacctgaa gccgctggca gaacgctggt
    22861 tagtggtggg tgacaaattt gttttaggtt ttgctcaatc cactgtcgag aaaagcttta
    22921 aagatgctgg actggtagta gaaattgcgc cgtttggcgg tgaatgttcg caaaatgaga
    22981 tcgaccgtct gcgtggcatc gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg
    23041 gcggaaaaac cctcgatact gccaaagcac tggcacattt catgggtgtt ccggtagcga
    23101 tcgcaccgac tatcgcctct accgatgcac cgtgcagcgc attgtctgtt atctacaccg
    23161 atgagggtga gtttgaccgc tatctgctgt tgccaaataa cccgaatatg gtcattgtcg
    23221 acaccaaaat cgtcgctggc gcacctgcac gtctgttagc ggcgggtatc ggcgatgcgc
    23281 tggcaacctg gtttgaagca cgcgcctgtt cccgtagcgg cgctaccact atggcgggcg
    23341 gcaagtgtac ccaggcggcg ctggcactgg cagaactgtg ctacaacact ctgctggaag
    23401 aaggcgaaaa agcgatgctt gctgccgaac agcatgtagt gactccggcg ctggagcgcg
    23461 tgattgaagc gaacacctat ttaagcggtg taggctttga aagtggtggt ctggctgcgg
    23521 cgcacgcagt gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg
    23581 aaaaagtggc atttggtacg ctgacgcagc tggttctgga aaacgcaccg gttgaggaaa
    23641 tcgaaaccgt agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg
    23701 atattaaaga agatgtcccg gcgaaaatgc gaattgtggc agaagcggca tgtgcagaag
    23761 gtgaaaccat ccacaacatg cctggcggcg cgacgccaga tcaggtttac gccgctctgc
    23821 tggtagccga ccagtacggt cagcgtttcc tgcaagagtg ggaataacct actccaaact
    23881 cccggcttgt ccgggagttt gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta
    23941 cgcaatctct gcaatatatt gaatttgcgt gcttttgtag gccggataag gcgttcactc
    24001 cgcatccggc actctgtgcc agtaatctga aactcccacg ttgttcagga attttttatc
    24061 cgcttctgga cattttctct acagaacaat cgttagcccg gaaggtcgaa tctgtgactc
    24121 ttattttcac ggacgaacca gatgaaagct tcactggcgc tgctcagcct gctaacggca
    24181 tttaccagcc actccttaaa atctcccgcc gttccaccaa ccgtggtaca aatccaggcc
    24241 aataccaacc ttgccatcgc agacggtgcc agacagcaaa ttggcagcac gctattttac
    24301 gatccggcgt atgtgcagct tacttatcct ggcggtgatg ttccgcaaga acgcggtgta
    24361 tgttccgatg tggtgatccg cgcattgcgc agccagaagg tcgatttgca gaaactggtt
    24421 cacgaagata tggcgaagaa ttttgctgag tacccgcaaa aatggaagtt aaagcgcccg
    24481 gacagcaaca tcgatcaccg tcgggtgcct aatctggaaa cctggtttag ccgccatgat
    24541 aaaacacgcc ccaccagcaa gaaccccagt gactatcaag cgggcgatat tgtctcctgg
    24601 cgactggata acgggctggc acatattggg gtggtttcag atggcttcgc ccgcgacggt
    24661 acgccgctgg tgatacataa cattggcgca ggcgcgcagg aagaagatgt gctgtttaac
    24721 tggcggatgg tggggcatta tcgttatttt gttaaataag agatcgcccc acatgaagaa
    24781 aaaattcaga tcctttttac actgacggct tcttttttct tacggctcac cagcgccagt
    24841 gccagcacaa tcagcacaat accgctacct tcaacggccc ccggattctc gccgagcagc
    24901 caccaggaaa agagtacgcc gcaaacggga acggccagtg tgcttaagct ggcaatactg
    24961 gcaggcaagt ttttcaatac aaacaaccat aagctccacg ccagtgccgt cgccagaatc
    25021 gcactgtagg ccagcgccca gaacacggtg ggctgccagt caatttcacg ttgcggcacc
    25081 agtaaagcga ccacactcat caccagcgcc gcatacagca tctgccagga tgttaacgac
    25141 aataaatcca cgcgcggatg acgggcatac agacgtttag caacaatcgc gctcgccccc
    25201 caactgacgc cggagaggat tgccagcatg gcacttttca tcgaagagaa atcgagttgc
    25261 cacggctgca acaccaaaaa taagccgaaa gcggcaatca gaatcgcgaa atattgccca
    25321 cgtcgcaggc gttcaccgag aaacaacgcg gcgaaaatca ccacccagaa cggcatggta
    25381 tagctcagga tcgccacctt ccccgcacct ccgctgacca acgcccactg cgccagacca
    25441 accatcccgc aggtttgtaa cagggcaatg gctaaggtgt atttaaacgg tgtcgggcgc
    25501 attccgcgac cacgtaataa aaggacgatg aataaaacga gagcgccgaa aatgcagcgt
    25561 aaggcggtaa agtcgaaggc accgatgtaa ctggtgactt gcttcatgaa aatccagcta
    25621 taactccaga taagtgtgag cacaaccagg ccactgattg ccagtgggtt gctctttcct
    25681 gcggcagaca tgagagatcc agtgtgtagt tttcgttcgc ccactatacg ctggtaaaga
    25741 acgttcagcg actgccagca agcagccgct gaacggggtc agattacagc aggtcgaaac
    25801 ggtcgaggtt catcactttc acccatgccg ccacgaagtc tttaacaaac ttctcgtggg
    25861 catcgctact ggcgtaaact tccgccaccg cacgcaggac ggagttagaa ccaaacacca
    25921 gatccgcacg gctggccgta aatttcactt cgccggtttc acggtcacgg ccttcgaaca
    25981 gctctttcga ttcgtcggtc gctttccact cgtaacgcat atccagcaag ttcacgaaga
    26041 agtcattgct caatacgcca acgcggtcag tgaagacgcc gtttttgctg ccatcgaagt
    26101 tggcacccag tacacgcatg ccgcccacca gcgcagtcat ttccggcgcg gtcagcgtca
    26161 gttgctgtgc tttgtcgatc agcagtgact cggtggtgga aacgtccaga cgagcgcgat
    26221 agttacggaa accgtcagca attggctcca gcagctcaaa catctcaatg tcagtctgat
    26281 cctgacgcgc atcaacgcga cccggcgcaa acggtacatg aatgctcaaa cctgcggcgc
    26341 ttgcggcttt ctcaacacca accacaccag ccagcactat gatatccgcc agcgaggctt
    26401 taccagactc tttctggatt ttctccagaa caggcagagc acgaacggct gcggcgttca
    26461 catcccagtc gcgctgcggc attaatgcca gacgcgcacc gttggcacca ccgcgtttgt
    26521 cgccaccacg gaaggtagaa gcagatgccc aggccaccga taccagctca ctaacagaca
    26581 gaccagaatc cgcaatcgcg aatttcagat cgataatgtc ctgctcggtc gggttgtaga
    26641 tcggctgcgg cagcggatct tgccagatca gatcttcttt cggcacttcc ggcccgatgt
    26701 agcgagattt cggccccata tccctgtgcg tcagtttgaa ccaggcacgg gcaaaggctt
    26761 cgttgaacgc ctgcggatcg ttgaggaaac gacgagagat cttctcgaac tcaggatcaa
    26821 aacgcagcgt caggtcggtc accagcattg tcggtttacg tttcttcgac ggatcaaacg
    26881 gatccgggat aatttccggt gcgtctaccg cttcgaactg gattgcgcca gccgggctgc
    26941 gggtctgtac ccactcatac ttgaacaggt tctcgaagaa atagttgctc cactgggtcg
    27001 gcgtctgggt ccagactact tccagaccag aggtaatggc atctgcgcca acgccgctgc
    27061 cgtaagtgct cgcccaacct aaaccttgtt cttcaatcgg tgcagcttct ggatcaggac
    27121 ctacatttga tgtcggaccg gcaccgtggg ttttacccag cgtatgacca cccgcaatca
    27181 gcgccacggt ttcttcgtcg ttcatgccca tgttgccgaa ggtcgcgcgg atagctgctg
    27241 ccgcagaaag cggttcgccg ctgtgatccg ggccttccgg gttaacgtaa atcagaccca
    27301 tctcggttgc acccagcggt gctttcgcca gcgcttccgg atgacggtga gtcagccagg
    27361 ctttttcatc accccagtta acatccagat ccggttccca gacgtcttca cgaccggcac
    27421 caaaaccgaa ggtacggaag ccggagtttt ctagcgccac gttacccgcg aggataaaca
    27481 ggtcggccca ggagattttc tgaccatatt tctgtttgat tggccacaac aggcgacgcg
    27541 ctttatcgag gcttacgtta tccggccagg agttcagcgg tgcaaaacgt tgctgaccac
    27601 gacccgcgcc accgcgtcca tcgattgaac ggtaagtccc cgcgccgtgc caggccatac
    27661 gaataaacag accggcgtaa ctgccccagt cggctggcca ccacggttga gattctgtca
    27721 acagggcttt cagatctttt ttcaggccgt agtaatctaa tttgctgaat tctttgcggt
    27781 agtcaaagtc ctcacccagt gggttagaac gattagaatg ttggtttaac aggtcaacac
    27841 gaagttgatt tggccaccag tcgcgagtgg ttgtgcccgc ccccgcactc tggtcgtgac
    27901 cgccctgatg gaacgggcat ttgccagtgg ctgtggtgtt atggatatcg tctgacgtgc
    27961 tcatcaatgt gctcccctct acagtgttac cgttacgata cacagcgtta gagagaagtt
    28021 ataattgaat taatccacgg atgcgatagt tgagatctta catattgttg gttaaagaga
    28081 tgtagatcaa attgatctta attaagcttt tttgattatg tccgaattcg gacaaatgct
    28141 ttataaaaaa gggttttagc ttcatatcct tcaagtaatg agggagaaaa caaggctgat
    28201 aaccttattt tcccgccctc atttcgaggc agcattttgt gctctgttta aaatttgtga
    28261 tcactgtgtg attttcacaa aagccacact atttataaac caggtcgaac ccccagcgta
    28321 tggcaaatcg cgtaactcat ttcagcacgg ttaagcgtat agaagtggaa atctttcact
    28381 ccttcacggc ttaaaatctt caccatatcc atggcaatat tcgcgccaac cagtttgcgg
    28441 gtttcggcat catcatccag accgtcgaac atttgcgcca tccacgccgg aatacgcacg
    28501 ttggtcatat cggcaaattt cttcgcctgt ttaaagttag ataccggcaa aattcccgga
    28561 ataatttcca catcaatgcc cgccgataca cagcggtcac gaaaacgcag gtagctttcg
    28621 acatcgaaga agaactgagt aatcgcgcgg ttggctccgg catccacttt gcgtttcaga
    28681 ttaagcaaat ccgcctgagc gctttttgct tccgggtgaa cttccggata cgccgccacg
    28741 gagatatcga aatctgccac ttcttttaat agcgtcacca ggtcagaagc atacatttct
    28801 ggcttaccac ttcccggcgg cagatcgcca cgcagcgcca cgatatgacg aataccgtta
    28861 ttccagtagt cgcgtgcaat ggtgcgcagc tcgtcgggcg tcgcatcaat gcaagtaaga
    28921 tgcggtgccg cttccagacc agtgcgatct ttaatgcctt taataatgct gtgcgtacgg
    28981 tcgcgctcgc cggagttcgc gccataggtc accgatacaa acttcggttt caggctgcta
    29041 aggcgatcga tggagttcca cagggtctgc tccatttcac tggtacgcgg cgggaaaaac
    29101 tcgaacgaaa cgttaatctg cccctggact tctgccaggc tctgattcag ggcatcccgc
    29161 tggctggcgt gaaaaaagct cataccttac ctcatcaatc gcttgtagtt atatgttgtg
    29221 ttgtgcacat ctatccgttt agacgtccag atgtaaagat gaaggaaaag ccgaagggcg
    29281 tcaaccgaaa aatgaccaat gatggtgagg aaaaatcagg gaagatgaaa aaacttcagg
    29341 atggtaagaa aaagaaaatc cggtatgcaa agcaaaccgg actgcaaata taaactaacc
    29401 atgtacttat tgcttcttgc cggattcgtc gtaaacgctt aatccggcct aatcctatga
    29461 taattcaata tatttcatta gaaaccataa acccgaaaac atgagtaccg ggcattatta
    29521 aatttctgaa attacaacaa ctgtgccagc cggttgatat ccgactgaat cgccccggcg
    29581 gtgacgtcgc gcccagcgcc aggtccgcgg atcaccagag ggttatcgcg ataccagcgg
    29641 ctttcgatgg caaagacgtt atcgcacggc agcagtgatg ccaacggatg atcttcacgc
    29701 accgcttcca cgcctacacg cgctttaccg ttggcatcga aacgcgccac gtagcgcagc
    29761 accagcccca tttcgcgggc cgcttccagc cgttgcacca tctgctcgtt cagttcatcg
    29821 ccattttcaa agaaatggtc gatgctgccg ccttcgcaat gagcaggcac cagcgattcc
    29881 acacgtacct gatccggttc gatgttgtaa cctgcttcac gcgccagaat caccagcttg
    29941 cgcatcacgt ctttgccaga gagatcgtca cgcgggtcag gttcggttaa gccctgctgc
    30001 cacgcctgat ccaccagctc ggtaaacggc acgctaccgt cgaattgcag gaacagccag
    30061 gagagcgtgc cggagaagat cccgctgatc gacaaaatag tatcgccgct gtcgatcaga
    30121 tcgcgcacgg tgtggttgat cggcaagccc gcaccgacgg tggcattgta cagccagtga
    30181 cgcccggttt tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc gctggctccc
    30241 gccagtttgt tggcgctgat aacgtggaaa ccgtggctgg cgaaatcaag atactgatca
    30301 gcaagctgct ggctggcggt aacgtccagc accactaaat catcatacgg atgggcgcgc
    30361 atccacagga acaacgactc ttcatcctgc tcaaccgctt catcgttgaa gaaggctaac
    30421 gcgcggctgg cgtccagccc gtcatagctc aacaggctgc ggcggctgtc caccacacct
    30481 gccagcacaa actcaaagcc ggtacgtgcc gaaagcgtgc tctgctcacg ggcgaacagt
    30541 tccagccaac gggaaccgat attgccctta ccgaacaata ccaggccgat gcgtttttct
    30601 gcgcggaaga cggactgatg cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt
    30661 actgccacca ggctgatgcc gtcatcggac tgccaggtaa attcgaccgg ctggcctttc
    30721 agttgctgcc agaagcggtg gcaatgcagc gggttacggg tgacgcctgc accgaccatc
    30781 gccaccagcg ccagcccctg acgcaggcgc agttcgccag gtaatcccgc ttcgtcgagg
    30841 attttcagcg cactgtcggc cacttctgag gtgtagcaaa attgcagcaa ctggcgatcg
    30901 ttatgtacgc caaccgccag cgggcgtacc tgcgcgcgtt tcaggatttg gtcgatctct
    30961 ttatgcgcca gtttgaaatc ctgactggcg ggcacctgaa actcaatcaa acagacatca
    31021 tcgtggctgg tgacaatacg cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg
    31081 gaaccttgat ccggcgtgta gctacagcgc agttgcaggt cgatttcgct gccagaaacc
    31141 ggctgtaaag tacgggcgtg aagaacggga gccgccaggc gcgccagttc gctggcctca
    31201 tccagacgca gcaacggcag caggcaggca tctttcactt tacgcgggtc ggcactgtat
    31261 accccggcga cgtcgctcca gatggttacg cgagaaacac ccgccagcgc accgatttgt
    31321 gtcgcggaat agtcggaacc gttacgcccc agcagcaccg tttcaccggc gttgttgcgg
    31381 ctgataaatc cggtcaccac cagacgtttg cccggatgtt gcaccagcag ctgttgcagc
    31441 aacgggtaag aaagcccttc atcaacctgc ggttgtgcgg cgcgttcagc gcgtaaaaac
    31501 tcgcgggcat caagccaggc cgctggcagc ccttgttgat taagtaccgc agacatcagg
    31561 cgcgccgacc acacttcacc gtggcccacc acttccgcat acaccgcatc gttaatacca
    31621 ctgtcgagca acgccgccag gcgttccaga tcgctgacaa aagcgctaat gaggctatcg
    31681 gcttcttcag cgggtagcag accgctaatc agatcgcact gatagcgacg cagcgtttgt
    31741 tgaacctgat gcgcagagag acgatcggtc tggcttagtt tcaaccagtt aatcaactgg
    31801 ttagtggtgc taccggcggc ggaaaccacc atcatatcgt caggctgaga gtactccgcc
    31861 ataatgcccg cgacacgcaa ataacacttc acatcagcca gactactgcc accaaattta
    31921 tgcagctgac gacctttcgc ccctgcctgc gcaatcacac tcatttttac cccttgtttg
    31981 cagcccggaa gccattttcc aggtcggcaa ttaaatcttc gccatcttca ataccggtgg
    32041 agatacgcag cagcgtctcg gagatcccgg cggcagcacg cgcttctggt gccatgcctg
    32101 catgtgtcat ggttgcggcg tgagagatta aactttccac tccccctaat gattccgcca
    32161 gcgtaaacaa cgacagcccg cccaggaaac gacgcagcgt ctgctcatcg ccatccagtt
    32221 caaaactcaa cattgcgcca aagccttttt gctggcgcgc ggcaatttca tgcccctgat
    32281 tttccggcag cgacgggtga tacagttttt tcaccaacgg ctgggtttgc agatatttca
    32341 caatcgcctg cgcgttacgc tgcgccagct ccatacgcgg caccagcgtt cgcaacccac
    32401 gtagcagcag atagctgtca aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg
    32461 ccagttcagt gacaacgtcc gggtctttag caatcaccac gccggccact acgtctgagt
    32521 gaccgttcag atatttcgtg catgaatgca acaccagatc ggcacctaat gccagcggat
    32581 tttgtaatgc cgggcttaag aaggtgttat ccaccacgct caccgccccg acttcccttg
    32641 ccagatggca gattttcgca atatccacga cgcgtaacaa tggattactt gggctttcta
    32701 ccagtaccag tttgggtttt tctgccagcg ctgcccgtaa tgcctgttca tcgccttgat
    32761 caacaaacaa cacgcgatag caaccacgtt tcgccaggct gtcgaacagg cgatagctac
    32821 cgccgtagca gtcgtgcggc gcaaccagca aatcgccagg tttcaaaaag acggtggtta
    32881 ccagatggat ggctgacatg ccggtattgg tgagcactgc accggcacca ccttccagtt
    32941 ctgccagcgc acgctgaacc acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg
    33001 cgcgcggttc attaaatccg gtaaagttat aggtgctgga aagatggatc ggtgggacaa
    33061 cgcaaccata ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg gcctgtttac
    33121 gcgtcatgtg atgaagttcc ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca
    33181 ttgtgttaat ggacgtcaat acatctggac atctaaactt ctttgcgtat agattgagca
    33241 aatcccaaat agccgttaaa attatatgca ttatcacgcc gacaggtgca ttacacgatg
    33301 tcacggtaac gcctgtacgg taaactatgc gggtttacgg tcagtaccca catcaactgt
    33361 gtggtctggt ctcaatttat tgacgaagag gattaagtat ctcatggctg aatggagcgg
    33421 cgaatatatc agcccatacg ctgagcacgg caagaagagt gaacaagtca aaaagattac
    33481 ggtttccatt cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt
    33541 gaacaacctg cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt
    33601 taccgggcaa cctttgccgg atgatgccga tctgcgtaaa gagcgcagcg acgaaatccc
    33661 ggaagcggca aaagagatca tgcgtgagat ggggattaac ccggagacgt gggaatacta
    33721 aagcaaaaaa gagcggcgcg gagtg
//
LOCUS       DACAPB010000029        32995 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.029, whole genome shotgun sequence.
ACCESSION   DACAPB010000029 DACAPB010000000
VERSION     DACAPB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.029"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..911)
                     /locus_tag="IT904_001242"
     CDS             complement(<1..911)
                     /locus_tag="IT904_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP3191053.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVVIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLA"
     gene            complement(1134..1706)
                     /gene="yeiP"
                     /locus_tag="IT904_001243"
     CDS             complement(1134..1706)
                     /gene="yeiP"
                     /locus_tag="IT904_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAP3191054.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1861..2115
                     /locus_tag="IT904_001244"
     CDS             1861..2115
                     /locus_tag="IT904_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP3191055.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEILTAP"
     gene            complement(2112..3293)
                     /gene="setB"
                     /locus_tag="IT904_001245"
     CDS             complement(2112..3293)
                     /gene="setB"
                     /locus_tag="IT904_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAP3191056.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            3661..4791
                     /locus_tag="IT904_001246"
     CDS             3661..4791
                     /locus_tag="IT904_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAP3191057.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVHAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPLAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4791..5729
                     /gene="fruK"
                     /locus_tag="IT904_001247"
     CDS             4791..5729
                     /gene="fruK"
                     /locus_tag="IT904_001247"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP3191058.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            5746..7437
                     /gene="fruA"
                     /locus_tag="IT904_001248"
     CDS             5746..7437
                     /gene="fruA"
                     /locus_tag="IT904_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAP3191059.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            7861..8802
                     /locus_tag="IT904_001249"
     CDS             7861..8802
                     /locus_tag="IT904_001249"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAP3191060.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNVANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDIRGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            8790..9728
                     /locus_tag="IT904_001250"
     CDS             8790..9728
                     /locus_tag="IT904_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAP3191061.1"
                     /translation="MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            9822..11072
                     /locus_tag="IT904_001251"
     CDS             9822..11072
                     /locus_tag="IT904_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP3191062.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11197..11841)
                     /gene="yeiL"
                     /locus_tag="IT904_001252"
     CDS             complement(11197..11841)
                     /gene="yeiL"
                     /locus_tag="IT904_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAP3191063.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALSMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQL"
     gene            11971..12912
                     /gene="rihB"
                     /locus_tag="IT904_001253"
     CDS             11971..12912
                     /gene="rihB"
                     /locus_tag="IT904_001253"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAP3191064.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            13012..14262
                     /locus_tag="IT904_001254"
     CDS             13012..14262
                     /locus_tag="IT904_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP3191065.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(14318..15406)
                     /locus_tag="IT904_001255"
     CDS             complement(14318..15406)
                     /locus_tag="IT904_001255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP3191066.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMILEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(15409..16266)
                     /gene="nfo"
                     /locus_tag="IT904_001256"
     CDS             complement(15409..16266)
                     /gene="nfo"
                     /locus_tag="IT904_001256"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP3191067.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(16340..17389)
                     /locus_tag="IT904_001257"
     CDS             complement(16340..17389)
                     /locus_tag="IT904_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAP3191068.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            17488..18369
                     /locus_tag="IT904_001258"
     CDS             17488..18369
                     /locus_tag="IT904_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191069.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            18574..20043
                     /gene="lysP"
                     /locus_tag="IT904_001259"
     CDS             18574..20043
                     /gene="lysP"
                     /locus_tag="IT904_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAP3191070.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            20337..22328
                     /gene="cirA"
                     /locus_tag="IT904_001260"
     CDS             20337..22328
                     /gene="cirA"
                     /locus_tag="IT904_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAP3191071.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(22360..23196)
                     /gene="yeiG"
                     /locus_tag="IT904_001261"
     CDS             complement(22360..23196)
                     /gene="yeiG"
                     /locus_tag="IT904_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAP3191072.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            23454..24122
                     /gene="folE"
                     /locus_tag="IT904_001262"
     CDS             23454..24122
                     /gene="folE"
                     /locus_tag="IT904_001262"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAP3191073.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            24139..25296
                     /locus_tag="IT904_001263"
     CDS             24139..25296
                     /locus_tag="IT904_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAP3191074.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRFFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            25438..26478
                     /gene="galS"
                     /locus_tag="IT904_001264"
     CDS             25438..26478
                     /gene="galS"
                     /locus_tag="IT904_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAP3191075.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            26758..27756
                     /gene="mglB"
                     /locus_tag="IT904_001265"
     CDS             26758..27756
                     /gene="mglB"
                     /locus_tag="IT904_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAP3191076.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            27817..29337
                     /gene="mglA"
                     /locus_tag="IT904_001266"
     CDS             27817..29337
                     /gene="mglA"
                     /locus_tag="IT904_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAP3191077.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGILSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            29353..30363
                     /gene="mglC"
                     /locus_tag="IT904_001267"
     CDS             29353..30363
                     /gene="mglC"
                     /locus_tag="IT904_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAP3191078.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(30434..31669)
                     /gene="preA"
                     /locus_tag="IT904_001268"
     CDS             complement(30434..31669)
                     /gene="preA"
                     /locus_tag="IT904_001268"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAP3191079.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(31663..32901)
                     /locus_tag="IT904_001269"
     CDS             complement(31663..32901)
                     /locus_tag="IT904_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP3191080.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         7970 a         8070 c         8738 g         8217 t
ORIGIN      
        1 taagcgagaa aagagtggct gccattaagc atccgcagtt tcatctcttc ccatggcagg
       61 acatcattca ccatttgtac acctgcgact tcccaggcag gacgcccagc gacgaagtta
      121 tcttccacca cccactggat aaacggttcg cagctaatcg cgcagggatc attcaccccc
      181 agatgctggc tgatttccac cagtgattcg tcggttgcag ccggaacaat gcggtcgacc
      241 atggttcccg gaaaactgac gtgctctttt atccacccgg cgagttctgg cgaacgtttt
      301 tctgccattc ccagcaccgc gtttttcacc acatgaccat tgtcgggaat attgtcgcag
      361 gagagcacgg taaacggtgt aaggccgcgc tcacggcggc gtttcagtgc ttcgacgaga
      421 atacccggtg cggagtgagg ttcttcaggg gtttgtagat cgtgaataat ccgcggatta
      481 ctggtgtcga gtgcaccggt ggccgggtca atacaatagc ctttttcggt aatcgtcagg
      541 gaaacaatta ccacctgtgg ctcgcaaaat ttctcaataa ttgccgctaa ggaatccagt
      601 tttgcattaa ggcattcgtg aacggcaccg acaattatca cctgattgcc gtccgcacct
      661 ttctccagca cggtatataa atggttctgt gcgcggagct ggctcatcag ttgatcaccg
      721 ctgaacaagc tgatttcaca gatcccccag tcgccgccct gggcattcag cacacgatcg
      781 gtcagtaacg cctgatgagc gcggtgaaag gcgccaaagc caaaatgaac gatacgtgat
      841 tgcaactgct ggcgatcgta gcgtggagcg tgtacatgat gcgggagcgt aacggaggca
      901 attgtcttca taggagtcac gtcttagtaa cgataaatta acaatgacag tgtaaagtta
      961 tatgacagca attgaaattg gtctggattg ttaatgcgat gaagtgtgag ggcgatcaat
     1021 caacgagtga aaattgaggg cgtaaggtgt tcaggaagcg caaaatagaa gattggtata
     1081 ataaattaaa aaagggacag cctgagctat cccttttctg catgcggctg aagttagtca
     1141 gcacgcccca tataacggcg ttcttcgata tggatacgaa ttttttcgcc cgggcttaag
     1201 tattctggta cctgaatcac cagaccagtg ctcaatgtcg ccggtttgtt acgggcgctg
     1261 gcagatgccc ctttgatgcc tggtgcggtt tcaacgattt ccagatcaac ggtctgcggc
     1321 agctcaagcg ccagcagttg accgtcccag gtcagcacct gcatgtccgg catgccgccc
     1381 tctggcataa acagcaactc ttcttcaatc tgatctttgg tgaaggtata cggggtatag
     1441 tcttctttat ccataaagac atattcgttg ccatcgacat aggagaagtc aacgtaacgg
     1501 cgggtcagcg tcacggtgtc aacgatatca tcacctttga aacgctcttc tactttcagc
     1561 ccggtacgga catcagaaaa acgcattttg tacagcgtag cggcaccgcg ggcagtgggc
     1621 gactgaatat caatatcctt aaccagcagc agtttgccgt tgtaattcag taccatacct
     1681 tttttaattt cgttcgctct tggcatcgaa aaattcctgt agtcgaaaat gtcaaaaata
     1741 tcgcgacaaa gtactcgcga cgggcctttc gggcaagtgg tattcgcact tttgctggtg
     1801 caaaaaaggt ggtactgtgc gcgctcatca atccggtggt taaccttaag agaacaacgt
     1861 atggaatgcc gtccgggttg cggggcgtgt tgcactgccc cttcaatttc cagccctatt
     1921 cccggtatgc cagatggcaa gcccgccaat acgccctgca ttcagcttga tgaacagcaa
     1981 cgctgtaaaa ttttcacctc gccactgcga ccgaaagtct gtgcaggctt acaagccagt
     2041 gctgaaatgt gcggtaactc gcgtcagcag gccatgacct ggcttatcga tctggaaatc
     2101 ctgaccgcgc cttaaacatc tttaatccgc agtaagcaaa acagagtggc gataatcatc
     2161 accatcgcaa accagaacac agcgtgataa ttccagatct cggcgacgat gcccgccact
     2221 gatcctgcga tgatccagcc cacgcgcgac gtgttggtat agagcgtggt ggctgaacct
     2281 gcctgaccgg gcatcaaatc ctgaaaatag agcatcccga tgccacccag aatgccaata
     2341 aaaatagcat ttagcagctg caagcccaac agaatgacag gtgaatgcgc catcagcatt
     2401 cctgcgtaaa aacagacgcc acccacggca gcaacgcgca ttaagaaacg cttacccaga
     2461 cgtttggcga aatatccggc aatcaacatg gtcgggattt ccagcccggc ggcggtcccc
     2521 atcatcacac cggccagttt ctcgggaaga tgcagttcgt tgataataaa tagcggcatg
     2581 ttgatgatgt acaggctgtt cgagccccac atcaatgtac aaatgacaaa cagcagcagc
     2641 gtatcgcgac ggttacggcg cggcgcttca atcgtgccgg tcgccagcgg aagctctttt
     2701 tgcatcgacg gtaaaaacag ccacaccatc acgccgcaaa caataaacgc taccgctgcg
     2761 ctcagataca ttaccgtaaa gctgaaaccc atcgctaagg cataagccag cggtgggcca
     2821 atgacccatg ccagtgaaac ctgagcgcgt aaaaaagaac tgaacatcac cgcctcacgt
     2881 ccggttttgt cggcatgttc acgggcaagg gcaaacattt gcgggttagc ggtcgagcca
     2941 aagctgctaa gaaagacgcc aacgaatagc aaaacaaagt agttgcgatt ccaggcaaaa
     3001 agggtgcagg ccagcacgcc taacaggcag caaaagacaa tcagcgattt gcgatcgccg
     3061 cgcttatcag agcgcccggc gagaaactga cttaccagaa tcccaatgac agcgctgccg
     3121 gtaaagaaga atcccaccat cgccggacgg gcatgtactt catcggtaag aaaaatactg
     3181 agtgtcgggg tttgcagagc gcccgcaata ccggtgagaa aggcaacgat taaaaacgcc
     3241 gtcgaggtca ggtcaaacga tttcgcgctg gagactgcgg gggagttatg catgctggtc
     3301 gatcactgga agaggaagac gcggagttta cgctgcttgt cagaaaataa acagacagcg
     3361 aaatcaaaag cgccacgaat aatcaaaatg ccatttcact gttgctgatt ggctgctgac
     3421 gctgctgaaa tgaaattgct gataagggat ttttccatca gcaattagga aaaatggcaa
     3481 aaaattgtgc agcacatcaa acttttgctc ataactttac ggctttcctt gcgtgctgaa
     3541 acagaagtat tatgctttct tgaaacgttt cagcgcgatc ttgtctttaa ccctaagcca
     3601 ggttggcgct ttttttctca tagaggctga atcgtttcaa ttcagcaaga gaggagaact
     3661 atgttccagt tatccgtaca ggacatccat ccgggcgaaa aggccggaga caaagaagag
     3721 gcgattcgcc aggtcgctgc ggcgctggtg catgccggta atgtagcaga aggctacgtc
     3781 aatggcatgc tggcgcgcga gcagcaaacc tcaacgttcc tcggcaatgg tattgctatt
     3841 ccacacggca ccaccgacac ccgcgatcag gtgctgaaaa cgggcgttca ggtatttcag
     3901 ttcccggaag gcgtcacctg gggtgacggt caggtagcgt acgtggcgat cggtattgct
     3961 gccagctcgg atgaacatct gggcctgcta cgccagctga cccacgtact gagcgatgat
     4021 tccgttgctg aacaactgaa gtcagcaaca acagcagaag aacttcgcgc attgctgatg
     4081 ggcgaaaagc agagtgagca gctgaagctc gacaacgaaa tgctgacgct ggatatcgtc
     4141 gccagcgatc tgctgactct tcaggcgctg aacgctgcgc gtctgaaaga ggcgggggca
     4201 gttgacgcca ctttcgtcac caaagccatc aatgaacaac cgctgaacct cggacagggt
     4261 atctggctga gcgatagcgc cgaaggcaat ctgcgtagcg cgattgcggt aagccgtgcg
     4321 gcaaatgctt ttgatgtgga cggcgaaacg gcagccatgc tggtgagtgt ggcgatgaat
     4381 gacgatcagc ccctcgcggt tcttaagcgt ctcgctgatt tgttgctcga caataaagct
     4441 gaccgcttgc tgaaagcgga tgcggcaacg ttgctggcgc tgctgaccag cgatgatgcg
     4501 ccgaccgacg acgtgttaag cgcggagttt gtggtgcgca atgaacacgg cctgcatgct
     4561 cgtccaggta ccatgctggt caataccatt aaacaattta acagtgatat taccgtgaca
     4621 aaccttgatg gcaccggcaa accggcaaac ggacgtagtc tgatgaaagt tgtggcactt
     4681 ggcgttaaga aaggtcatcg cctacgcttt accgcccagg gtgcagatgc tgaacaggcg
     4741 ctgaaagcaa tcggcgacgc tatcgctgct ggtcttgggg agggcgcata atgagcagac
     4801 gtgttgctac tatcaccctt aatccggctt atgaccttgt tggtttctgc ccggaaattg
     4861 aacgcggcga agtgaacctg gtgaaaacca ccggtctgca tgcggcgggt aaaggcatca
     4921 acgtggccaa agtattaaaa gacctgggaa ttgatgtcac cgttggcggc ttcctcggta
     4981 aagacaatca ggatggtttt cagcaactgt tcagcgagct gggcattgcc aaccgtttcc
     5041 aggttgtaca ggggcgcact cgaattaacg ttaagctgac ggaaaaagac ggcgaagtga
     5101 ccgacttcaa cttctcgggt tttgaagtca cccccgccga ctgggaacgc tttgtgactg
     5161 attctctgag ctggctcggt cagttcgata tggtctgtgt cagcggaagc ttaccgtcag
     5221 gcgtcagccc ggaagcgttc accgactgga tgactcgcct gcgtagtcag tgtccttgca
     5281 ttatctttga tagtagccgt gaagcgttag tagcaggttt gaaagcggca ccgtggctgg
     5341 tgaaacctaa ccgccgcgag ctggaaatct gggcaggccg taaactgcct gaaatgaaag
     5401 atgtgattga agctgcgcat gcgctgcgtg aacaaggtat cgcgcatgtt gttatttcac
     5461 tgggtgccga aggcgcgctt tgggttaatg cctccggcga atggatcgcc aaaccaccgt
     5521 cagtcgatgt cgtaagcacc gttggcgcag gggattctat ggttggtggc ctgatttatg
     5581 gcttgctgat gcgtgaatcc agtgaacaca cactgcgtct ggcgacagct gttgcagccc
     5641 tggcggtaag tcaaagcaat gtgggtatta ccgatcgtcc gcagttggcc gcaatgatgg
     5701 cgcgcgtcga cttacaacct tttaactgac agcaggagag gcataatgaa aacgctgctg
     5761 attattgacg ctaatctcgg tcaggcacgc gcctatatgg cgaagaccct gctgggcgcg
     5821 gcggcgcgaa aagcaaaact ggaaatcatc gacaatccga acgacgctga aatggcgatt
     5881 gttctcggtg attccatccc gaatgacagc gcgctgaacg gtaaaaatgt ctggctgggt
     5941 gatatttccc gggcagttgc gcaccctgag ctgttcctga gtgaagccaa aggccatgcg
     6001 aaaccttaca ctgcgccggt cactgcgaca gcaccggttg ccgccagcgg tccgaaacgc
     6061 gtagttgcgg tgactgcttg cccgactggc gtagcacaca cctttatggc ggctgaagcc
     6121 attgaaaccg aagcgaaaaa acgtggctgg tgggtgaaag ttgaaacccg tggttctgtt
     6181 ggcgcgggta atgcaatcac tcccgaggaa gtagccgcag cggatctggt gattgtggcg
     6241 gcagatatcg aagtggatct ggcgaaattt gctggtaaac cgatgtatcg tacctctacc
     6301 ggtctggcgc tgaagaaaac cgctcaggaa ctggataaag cggttgctga agcaacgccg
     6361 tatgaaccgg cgggcaaagc tcaaacggcg accactgaag gtaagaaaga gagtgcaggc
     6421 gcttatcgtc acttgctaac gggcgtctct tacatgctgc cgatggtcgt tgctggtggt
     6481 ctgtgtatcg cgctttcttt tgcttttggt atcgaagcgt ttaaagagcc gggtacgttg
     6541 gcagcggcgc tgatgcagat tggtggtggt tcagcctttg cgctgatggt gccggtactg
     6601 gcaggttata ttgccttttc cattgccgat cgtccgggcc tcacgccggg tctgattggc
     6661 ggtatgctgg cggtcagcac cggttctggc ttcattggcg gtattattgc gggcttcctg
     6721 gctggttaca ttgcgaagtt aatcagtacg caactgaaac tgccacagag tatggaggcg
     6781 ctgaaaccaa tcctgatcat tccgctaatt tccagtctgg tggtcggtct ggcgatgatc
     6841 tacctgatcg gtaaaccggt tgctggcatt ctcgaagggc tgactcactg gctgcagacc
     6901 atggggactg cgaatgcggt tctgctgggg gcgatcctcg gtggcatgat gtgtactgac
     6961 atgggcggtc cggtaaacaa agcagcgtac gcattcggtg tgggtctgct gagtactcaa
     7021 acctatggcc cgatggcggc gattatggcg gcaggtatgg tgccaccgct ggcaatgggt
     7081 ctggcaacaa tggtggcgcg tcgcaaattc gacaaagcgc agcaggaagg tggcaaagcc
     7141 gctctggtat tgggactgtg cttcatttcg gaaggtgcaa ttccgtttgc tgctcgtgat
     7201 ccgatgcgtg tgctgccgtg ctgtatcgtg ggtggtgcgc tgactggcgc aatctcaatg
     7261 gcgattggtg cgaaactgat ggcaccacac ggtggtctgt ttgttctgct gatccctggc
     7321 gctattacgc cggtactggg ttacctggta gcaattattg ccggtacgct ggtggcgggt
     7381 ttggcctatg ccttcctgaa acgtccggaa gtggacgcag tagcgaaagc agcgtaataa
     7441 aaggtgtgtt acagggcaga aatttcctgc cctgtatcat tggatgagcg caaagggaat
     7501 gaccagcttg tgaatgtaat tttatgaatt taaatggttt tatctttcag tagaaacttg
     7561 aaacgttcat caaacactaa ggttatttcc tcaagggcag attattttct gccctttttt
     7621 attgttcctg ttctgtgcat cccgtcacaa attcctcgct tttgtaattc aggcatttaa
     7681 tcgcattttg tgagcctgct caaatctgcc catttagcct ctctggcggt aatttacttt
     7741 acgtaaaaca tgcatactat gagcacatgt ttaaatatga acacatgttt aaaccgtctt
     7801 tgcagtatct atattcttgt ggattgacag ggttggtaat tttttaccag aggacgtgct
     7861 atgcgcgaaa aggattatgt cgtaattata ggttcggcga atattgatgt cgccggatat
     7921 tcacatgaat cattaaatta tgcggattca aatccaggta aaataaaatt tacgcctggt
     7981 ggagtagggc gcaatattgc acaaaacctg gcgttgctgg gtaacaaagc ctggctactg
     8041 agcgcagtag gcagtgattt ttatggtcaa tcgctgctaa cgcaaaccaa tcaatctggc
     8101 gtttatgtcg ataaatgcct gattgtgccg ggagaaaata cgtctagtta tttatcatta
     8161 ctcgataata ccggtgaaat gctggttgct ataaatgaca tgaatattag caacgctatt
     8221 acagctgaat atctcgcaca gcaccgtgaa tttattcaga gggcaaaggt cattgttgcg
     8281 gactgtaata tcagtgaaga ggcactggca tggattctgg ataatgtcgc caacgtaccc
     8341 gtatttgtcg atccggtttc cgcatggaaa tgtgtcaaag tgcgcgaccg tctaaatcag
     8401 atccacactc tgaagccaaa ccgccttgaa gcggaaaccc tgagtgggat tgcgctgtca
     8461 gggcgtgacg atgtggcaaa agttgctgcc tggttccatc aacatggcct gaaccgactg
     8521 gtattgagca tgggcggcga cggcgtttat tacagcgata tcagaggtga aaatggctgg
     8581 tctgcgccga tcaaaaccaa tgttattaat gttaccggag cgggcgatgc catgatggcg
     8641 ggacttgctt cgtgttgggt agacggaatg ccgtttgccg aatctgttcg tttcgcacag
     8701 ggatgttcgt caatggcgct ctcctgtgaa tacaccaata accccgattt atcgattgcc
     8761 aacgttatat cgttagtgga gaacgcagaa tgtctaaatt aaaaatttcc cctgaattat
     8821 tacaaatttc cccggaagtg caggaagctt taaaaaacaa aaaaccggtt gtggcgctgg
     8881 aatcgaccat tatttctcac gggatgccgt tcccacaaaa tgcccagacc gcaattgaag
     8941 tagaagaaac tattcgtaaa cagggcgcag tacctgccac tatcgccatt attggcggcg
     9001 tgatgaaagt gggtttaagt aaagaagaaa ttgaattact gggtcgtgaa gggcataacg
     9061 tgaccaaagt tagtcgtcgc gatttacctt ttgttgttgc cgccggaaaa aatggcgcaa
     9121 ccactgtggc ttcaacgatg attattgcgg cgcttgccgg aattaaagta tttgccaccg
     9181 ggggaattgg tggtgtgcat cgcggggcgg aacatacctt cgatatttct gccgatttgc
     9241 aagaactggc aaatactaat gtcaccgttg tttgtgccgg ggcgaaatct attctcgatt
     9301 taggattaac cactgagtat ttagaaacct tcggtgtgcc gttaattggc tatcagacta
     9361 aagcgctgcc tgcgtttttc tgccgtacca gctcgtttga cgtcagcatt cgtctcgaca
     9421 gcgccagtga aattgcccgt gcaatggcgg tgaaatggca aagcgggctg aacggtggcc
     9481 tcgtggtagc gaacccgatc ccggaacagt ttgcgatgcc ggaggaatct atcaatgcag
     9541 ccatagatca agccgtcgcc gaagccgaag agcagggcgt tattggtaaa gaaagtacac
     9601 cgttcctgct ggcgcgcgtt gctgaactga ccggcggtga cagcctgaaa tccaatatcc
     9661 agctggtgtt caacaacgcc attctggcga gtgaaattgc caaagaatac cagcgtctcg
     9721 cgggttaatt tctgacgggc agaaagccgt cgcccgtact catcaaacat accaaaacct
     9781 ggcgctgacg ccgggtttac tggcatgaag ggaaaatcat tatggacata atgagaagtg
     9841 ttgtggggat ggtggtgtta ctggcaatag catttctgtt gtcagtgaat aaaaagagca
     9901 tcagtttgcg tacggttgga gccgcactgc tgctgcaaat cgctattggt ggcatcatgc
     9961 tctacttccc accgggaaaa tgggcagtag aacaggcggc attaggcgtt cataaagtga
    10021 tgtcttacag tgatgccggt agcgccttca tttttggttc gctggttggg ccgaaaatgg
    10081 atgtcctgtt tgacggtgcg ggttttatct tcgcctttcg cgtacttccg gcgattattt
    10141 tcgttactgc gctcatcagt ctgctgtact acattggcgt gatggggctg ctgattcgca
    10201 tccttggcag cattttccag aaagctctta acatcagcaa aatcgaatct tttgtcgcag
    10261 tcacaactat tttcctcggg caaaatgaga tcccggcgat cgttaaaccg tttatcgatc
    10321 gcatgaatcg caacgagttg tttaccgcaa tttgtagcgg gatggcgtcc attgctggtt
    10381 cgatgatgat tggttatgcc ggaatgggcg taccaattga ctacctgtta gcggcatcgc
    10441 tgatggcgat cccaggtggt attttgtttg cacgtattct tagcccggcc accgagcctt
    10501 cgcaggtcac atttgaaaat ctgtcgttca gcgaaacgcc gccaaaaagc tttatcgaag
    10561 cggcggcgag cggtgcgatg actgggctga aaatcgccgc tggtgtagcg acggtggtaa
    10621 tggcgtttgt cgcaattatt gcgctgatca acggcattat cggcggaatt ggtggctggt
    10681 ttggtttcgc caatgcctct ctggaaagta tttttggcta tgtgctggca ccgctggcgt
    10741 ggatcatggg tgtggactgg agtgatgcca atcttgcggg tagcctgatt gggcagaaac
    10801 tggcgattaa cgaattcgtc gcttacctga gtttctcccc atacctgcaa acgggcggca
    10861 cgctggaagt gaaaaccatt gcgattatct cctttgcgct ttgtggtttt gctaactttg
    10921 gttctatcgg tgttgtcgtt ggcgcatttt cggctatttc gccaaaacgt gctccagaaa
    10981 tcgcccagct tggtttacgg gcgctggcag cagcaacgct ttccaacctg atgagtgcga
    11041 ctattgccgg gttctttatt ggtctggcgt aatgctcgat ttgtagaccg gataaaacgt
    11101 attaagcgtt gcatccggca atcgcgctat tgcaaataat ttttactgca tcatcccgga
    11161 gaatttattc tccgggtcca tctccagcgc cagtccttac aactgctttc tgtttttaat
    11221 gagataccct ttcttgcttt tagttaataa accgtcgtga atgaattgcg cgagaacata
    11281 taaaagatgt cgataagaaa cgcctaaata ctctgccgct tgcgtatgct tttcgtgata
    11341 aagatcacct tcctgcgaga gtaatataaa agctgccagg cgattaacta acggaaatga
    11401 ttgattctga gttaaagaaa caatattacg ataattttta tgacttaagg tgacgcagag
    11461 ttttcgtaaa aatagcgtgt cgtttaataa cagcgggcgg taatgtttca tagagagcgc
    11521 aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt tcatggtctt tgtcgattaa
    11581 ctcaatctca ccaataaaac agggggcggc aaagaaatcg atcagcgaca cgcgaccatt
    11641 agcaagcgtg gcgtaaagcc tggcgcggcc tcgcgtcaga taaaacagcc aggacggctg
    11701 ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga aacagccgcg tatcggctga
    11761 aacatccgtt aaaaagcaat ccttaaacgc ggattcactc atcaattgct gcttaagatc
    11821 attattgtgg atttctttca cgatgtttat ccgctatgag atttctcata tttagcatac
    11881 gtacgcggtt ggtaagattc aatcatcggc ccggtcgaat tttctttgct gtagaaaaaa
    11941 taacgcaact ggaaacagag gaaataaaca atggaaaaga gaaaaattat tctggattgt
    12001 gatccgggtc atgatgatgc tattgcaatg atgatggcag cgaaacatcc ggcaatagat
    12061 ttattaggca tcactattgt agcgggtaat cagacgcttg ataaaacatt aattaatggc
    12121 ctgaatgttt gccagaaact ggagattaat gttccggttt atgcggggat gccgcaaccc
    12181 attatgcgta aacaaatcgt tgccgataat attcacggtg aaaccggact ggatggcccg
    12241 gtattcgagc cgctgacccg ccaggcagaa agtacccacg cggtgaaata catcattgat
    12301 accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct ttcaaatatc
    12361 gcggtggcaa tgcgtatgca acccgcgatc ctgcccaaaa tccgtgaaat tgtgctaatg
    12421 ggcggcgctt acggtacagg caacttcacg ccatctgccg agttcaacat ctttgctgat
    12481 ccggaagcag cccgcgtggt gttcacctcc ggcgttccat tagtgatgat gggcctcgat
    12541 ctcaccaacc agaccgtttg caccccggac gtgattgcac ggatggaaag ggcaggcggc
    12601 cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaaaac
    12661 tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct gattaaccct
    12721 gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg cccttgctat
    12781 gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc ccgctaatac caaagtcggc
    12841 atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc
    12901 aaaacccatt aagtgttgat aacaagccgg gtcagtatcg caagatccaa gcccggctat
    12961 ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataataac tatggatgtc
    13021 atgagaagtg ttctgggaat ggtggtattg ctgacgattg cgtttttgct gtcagtaaac
    13081 aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc
    13141 gggattatgc tttggttacc gccagggcgt tgggtcgctg aaaaagtcgc ttttggcgtg
    13201 cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc gctggtcggg
    13261 ccgaaaatgg acacgctgtt tgatggcgca ggatttatct ttggtttcag ggtattaccg
    13321 gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatt
    13381 ttaattcgca ttctcggcgg tatattccag aaagcattaa atatcagcaa gattgagtca
    13441 ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc
    13501 tttatcgatc gtctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg
    13561 attgctggtt cgacaatgat tggttacgcc gccctgggcg tacctgtgga atatttgctg
    13621 gcggcatcgt taatggcgat cccaggcggg atcttgtttg cccgcctgtt aagcccggct
    13681 acggaatctt cgcaggtttc ctttaataac ctctctttca ccgaaacacc gccaaaaagc
    13741 attattgaag ccgctgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg
    13801 acagttgtta tggcatttgt cgccatcatt gcgttaatta atggtattat cggcggcgtt
    13861 ggcggctggt ttggttttgc acatgcctcg ctggagtcca ttttaggtta cctgttggcc
    13921 ccattggcgt gggtgatggg ggttgactgg agtgatgcaa atcttgccgg gagtttgatt
    13981 gggcagaagc tggcgatcaa tgaatttgtc gcttatctca atttctcgcc atatctgcaa
    14041 acgggtggca ctctggatgc taaaaccgtg gcgattattt ctttcgcgtt gtgcggtttc
    14101 gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt
    14161 gcgccggaaa tcgcccaact tggtttacgc gcgctggcgg cggcgacact ttctaacctg
    14221 atgagtgcta ctattgcagg attctttatt ggtttagcgt agtgcgcaat ttatgagccg
    14281 gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaaa
    14341 gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgc gaaattgccg
    14401 cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta tccttcaaga aagctatata
    14461 ccaggcccgc cataaaacca tcgtcagcac caaaactgtc tactgtcgta tgcgctggcg
    14521 cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca tcgggcaaat
    14581 aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag
    14641 catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg
    14701 tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga
    14761 caaacaccgg gatttcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt
    14821 tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt
    14881 gcggtgtcaa ctgttccagc agatgggtat cgttaatcgc cagcacggtt tgatcgtctc
    14941 gattggcaat tgccagatac gtcgatgtgc tttgaccatg caaacgaacg cagccggaga
    15001 cattcacgcc ggcacggcgc gtttcttcca ggatcatttc gccataaaag tcatcgccaa
    15061 tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg atgttgcgtc
    15121 ccacgccgcc cgccgagcaa tgaattgtac cgggatgaga agccgcttgc gggtaacgga
    15181 tatccgccat cccgcgaata tccatattga ttgtccccac cactacgcag tattcctgct
    15241 cggtgagaat gtaacctttg cctttaatcc gccctttgcg cattaaatcc ataatatgcg
    15301 ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat ttcgttctgc tgaatcagcg
    15361 ggttacgccg taaaattgca aggatctcct tttcccggtt attcatcttc aggctaccgc
    15421 tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat
    15481 ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc agcggaacgc
    15541 atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt
    15601 gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccaacaa tacgggcaaa
    15661 atccgcgaac gttttctcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc
    15721 atggcaggta tcaatgcaga cgccgacacg ggatttatct tccacgccgt cgataatcgc
    15781 cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt tttctatcac
    15841 cgctgtcaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag
    15901 gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag
    15961 ccccagctgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc
    16021 ttcagtgacc ggatgtccga ggttaatcag ataactgtcg tggggaagaa tttgcgccga
    16081 tgtgtagtga tatttttcac aggcggcttt gaattcatcg atggtttgcg tcgtgagcgg
    16141 tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc
    16201 ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta
    16261 tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac
    16321 aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac
    16381 caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc gccccggctt
    16441 ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga
    16501 aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa
    16561 agatggcaac tacgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc
    16621 cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca
    16681 gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc gggctgatgg
    16741 catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga
    16801 aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatcg
    16861 cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt
    16921 tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga
    16981 tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca
    17041 ggaaggtact ggacagcgtc aacacgtcaa tgatgatccc actgataccg acatcggcaa
    17101 tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct
    17161 tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta
    17221 aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg
    17281 gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa
    17341 taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct
    17401 ttaaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta tatatttata
    17461 attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt ggaagttttt
    17521 gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa
    17581 tcagcagtga gcgcagcctt gaccgacctg gaagggcagc ttggcgtgca actgtttgat
    17641 cgcgtgggga aaagactggt tgttaatgaa cacgggcggc tgctctatcc gcgtgcgttg
    17701 gcattgcttg aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt
    17761 cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat cgcccgttat
    17821 cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaatagcca ggacgtgatg
    17881 caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg ccacagcact
    17941 gaaatcattt ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg
    18001 ccgttggccc gtggtccggt caccttagaa cagctggccg ctgcgccgtg gatcctgcgt
    18061 gaacgcggtt ccggcacgcg ggagattgtc gattatctgt tgctgtcaca tttaccgaag
    18121 tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg
    18181 ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc aattgcaggc aggcacatta
    18241 agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg
    18301 caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc cgcaaatgtg
    18361 ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc
    18421 ttcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc tgggcgatca
    18481 tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc
    18541 gcgcctcatt tttaagatgg atagcatttt tgtatggttt ccgaaactaa aaccacagaa
    18601 gcgccgggct tacgccgtga attaaaggcg cgtcacctga cgatgattgc cattggcggt
    18661 tccatcggta caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc
    18721 ggggcattgc tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc
    18781 ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat
    18841 gttgaagaag gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact
    18901 atcgccgttg acctggttgc agctcagctg gtcatgagct ggtggttccc ggatacaccg
    18961 ggctggatct ggagtgcgtt gttcctcggc gttatcttcc tgctgaacta catctcagtt
    19021 cgtggctttg gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc
    19081 tttatcatcg ttggcgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc
    19141 tggagcaact ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc
    19201 gtagctatga ttgtcggctt ctctttccag ggaaccgagc tgatcggtat tgctgcaggc
    19261 gagtccgaag atccggcgaa aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc
    19321 ctgttgttct atgtgttcgc gatcctgatt atcagcctga ttattccgta caccgatccg
    19381 agcctgctgc gtaacgatgt taaagacatc agcgttagtc cgttcaccct ggtgttccag
    19441 cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag ttattctgac ggcggtgctg
    19501 tcagcgggta actccggtat gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac
    19561 ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg
    19621 tatgcgacga cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg caaccagacg
    19681 gtatacctgt ggctgctgaa cacctccggg atgacgggtt ttatcgcctg gctggggatt
    19741 gccattagcc actatcgctt ccgtcgcggt tacgtattgc agggacacga cattaacgat
    19801 ctgccgtacc gttcaggttt cttcccactg gggccgatct tcgcattcat tctgtgtctg
    19861 attatcactt tgggccagaa ctacgaagcg ttcctgaaag atactattga ctggggcggc
    19921 gtagcggcaa cgtatattgg tatcccgctg ttcctgatta tttggttcgg ctacaagctg
    19981 attaaaggaa ctcacttcgt acgctacagc gaaatgaagt tcccgcagaa cgataagaaa
    20041 taagtttcct cccttccttg ctaagccctc tcaaccgaga gggctttttc aattccattt
    20101 ccctgacaaa tcatgcggat ataaaattta acatttggat tgataattgt tatcgtttgc
    20161 attatcgtta cgccgcaatc aaaaaaggct gacaaatcag aggctgttcc ggctttctgg
    20221 gatgatcacc tgcataaaaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtga
    20281 agaagatgtg agcgataacc cattttattt tcgtagttac ctcatggaga tatggaatgt
    20341 ttaggttgaa ccctttcgta cgggtcgggc tgtgtttgtc cgctatttct tgtgcatggc
    20401 ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct tccgtggaac
    20461 aaaatcttaa agatgcacct gccagtatca gcgtcattac ccaggaagac ctgcagcgaa
    20521 aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg
    20581 aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctat accctgattc
    20641 tcgtcgacgg taaacgcgtg aactcccgca atgccgtctt ccgccacaat gatttcgatc
    20701 tgaactggat cccggtcgat tccatcgaac gtattgaagt ggtccgtggc ccgatgtcgt
    20761 cgctgtacgg ttccgatgcg ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc
    20821 agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc gatcgcggtg
    20881 acacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg ctgggaatga
    20941 aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg
    21001 ataccggaga aacgccgcgt attgaaggat tctccagccg cgacggcaat gtcgaatttg
    21061 cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac cgtcaggatc
    21121 gtgattccga ctcgctggac aaaaaccgcc tggaacgcca gaactactcc gtcagccata
    21181 atgggcgttg ggattacggc accagcgaac tgaaatacta cggtgagaaa gtcgagaaca
    21241 aaaaccctgg caacagcagc ccgataactt ccgaaagcaa tacggtcgac ggcaaataca
    21301 cgttgccgct gacggcgatt aatcagtttc tcacggttgg cggtgaatgg cgtcacgaca
    21361 aacttagcga tgcggtgaac ctgaccgggg gaaccagctc caaaacgtct gccagccagt
    21421 acgcgctgtt tgtggaagat gaatggcgga tcttcgagcc gctggcgctg acgaccggcg
    21481 tgcgtatgga cgatcacgaa acctacggtg aacactggag tccgcgtgcc tacctggttt
    21541 ataacgccac cgacaccgta acggtgaaag ggggctgggc gacggcattt aaagcacctt
    21601 ctctgttgca acttagccct gactggacga gcaattcctg ccgtggcgca tgtaagattg
    21661 tgggtagccc ggatctgaaa ccagaaacca gcgaaagttg ggagctgggg ctttactaca
    21721 tgggtgaaga aggctggctg gaaggggttg aatccagcgt taccgttttc cgtaacgatg
    21781 tgaaagatcg tatcagcatc agccgtacgt ctgacgtcaa cgctgcaccg ggctaccaaa
    21841 actttgttgg ttttgagacg ggcgctaacg gacggcgcat accggtattt agctactaca
    21901 acgttaacaa agctcgtatt cagggcgtgg aaaccgaact gaaaattccg ttcaacgatg
    21961 aatggaaact gtcgatcaac tacacctaca acgatggtcg tgatgtcagc aacggcgaaa
    22021 acaaaccgct atccgatctg ccgttccata ctgctaacgg tacgctggac tggaaaccgc
    22081 tggcgctgga agactggtca ttctatgttt ctgggcacta taccgggcag aaacgcgccg
    22141 acagcgcgac ggctaaaaca ccgggcggtt ataccatctg gaataccggc gcggcctggc
    22201 aggtgactaa agacgtcaaa ctgcgcgcag gcgtgctgaa ccttggcgac aaggatctca
    22261 gtcgtgacga ctacagctat aacgaagacg gacgtcgtta ctttatggca gtggattatc
    22321 gcttctgatg agaagatgcc cggcgaaccg ggcggacttt cacttcagta aatactgcgc
    22381 atggaagcgc aggtgatcct ctataaaaga ggcgatgaag tagtaactgt gatcatatcc
    22441 cggctgaata cgcagcgtca tcggccacgc tttctggcgc gcggcttctg ccagtaccgc
    22501 aggttgcaac tggtcggcaa gaaactgatc attatcgccc tgatcgataa gcgtcgggat
    22561 cgcatcctgc gcgttactgg catacatcag tgcgcaactg tcccattcca gccatgcatt
    22621 tttgtcctca cctaaatagc tgctaaacgc tttgattccc cacgggacgc tgcacggatt
    22681 cacaattggc gcaaaggccg aaacgctggt gtatttaccc ggatttttca gcgccataat
    22741 cagcgcaccg tgaccaccca ttgagtgacc gctaatggcg cagcggtcgc tgacattaaa
    22801 ttgcgactga accagcgccg gtaattcatc gcgcagataa tcatacatcc ggtaatgcgt
    22861 cgcccacggc ggttgcgtgg cattaagata aaagcctgcg ccctggccta aatcgtagcc
    22921 atcgtcgttg gcaacctttt cgccgcgcgg gctggtgtct ggcatcacca gtacaatccc
    22981 cagttccgcc gctacccgct gggcacccgc cttggtggtg aagttctcgt cattgcaggt
    23041 taatccggaa agccagtaca gcactggtgg cggagtgtga tcacgaggtg gagggagaaa
    23101 gatactgaac gtcatcgggc agtttaaggt actggagtcg tgtcgccagc gttgctgcca
    23161 gccttcaaaa cagcggtgct cttcgagcat ttccatgcgt ggctccttgt tgtgttgttt
    23221 gcaatttctt catcataccg tttattcaat gtgctgtgag taactttcac ttccgtattt
    23281 gcataacgat gttttaacat ctgctgatga aaggcagcgg caattacaat aattatcgct
    23341 gtgaatactg gattatgtgc gccgcctcac gcacaataat caggctgtaa atcagcttaa
    23401 taactttgcc cccacgcagg gcggaggcgt cacacctgca ggagaaatca taaatgccat
    23461 cactcagtaa agaagcggcc ctggttcatg aagcgttagt tgcgcgagga ctggaaacac
    23521 cgctgcgccc gcccgtgcat gaaatggata acgaaacgcg caaaagcctt attgctggtc
    23581 atatgaccga aatcatgcag ctgctgaatc tcgacctggc tgatgacagt ttgatggaaa
    23641 cgccgcatcg catcgctaaa atgtatgtcg atgaaatttt ctccggtctg gattacgcca
    23701 acttcccgaa aatcaccctc attgaaaaca aaatgaaggt cgatgaaatg gtcaccgtgc
    23761 gcgatatcac tctgaccagt acctgtgaac accattttgt taccatcgat ggcaaagcga
    23821 cggtggctta tatcccgaaa gattcggtga tcggtctgtc aaaaattaac cgtatcgtgc
    23881 agttctttgc ccagcgtccg caggtgcagg aacgtctgac gcagcaaatt cttattgcgc
    23941 tacaaacgct gctgggcacc aataacgtgg ctgtctcgat cgacgcggtg cattactgcg
    24001 tgaaggcgcg tggcatccgc gatgcaacca gtgccacaac aacgacctct cttggtggat
    24061 tgttcaaatc cagtcagaat acgcgccacg agtttctgcg cgctgtgcgt catcacaact
    24121 gattaaaagg caggaaccat ggagcgcaac gtcacgctcg attttgttcg cggcgtcgcc
    24181 attctgggga tcctgctatt aaacatcagc gcctttgggc taccaaaggc ggcttatctc
    24241 aatcccgcct ggtacggcgc tattacgccg caggatgcat ggacctgggc atttctcgat
    24301 ctcatcggcc aggtgaaatt cctcacgctt tttgcgctgc tgtttggtgc gggcctgcaa
    24361 atgttgctgc cccgtggcag acgctggatc cagtcgcggt taacgctgtt agtcttgctg
    24421 ggttttattc acggtttatt gttctgggac ggcgatattc tgctggctta cgggctggtg
    24481 ggcttaatct gctggcggct ggtgcgcgat gcgccatcgg taaaaagcct gtttaataca
    24541 ggcgtcatgc tttatctggt ggggcttggc gttttgctgt tattggggct gatttccgac
    24601 agccagacca gccgcgcctg gacgccggat gcatcggcta ttttgtatga aaaatactgg
    24661 aagcttcacg gcggcgttga tgcgatcagt aatcgtgccg atggtgttgg caacagttta
    24721 ctggcactgg gcgcacagta tggctggcaa ctggctggga tgatgctcat tggtgccgca
    24781 ttgatgcgca gtggctggct gaaagggcag ttcagcttac gtcactatcg tcgtactggt
    24841 tttgtgctgg tggcgattgg ggtgatcatt aaccttcctg ccatcgccct gcaatggcgg
    24901 ctggactggg catatcgctg gtgcgccttc ttacttcaga tgccgcggga actgagtgcg
    24961 ccgtttcagg cgataggcta tgcgtcgctg ttttatggtt tctggccgca attgagccgc
    25021 tttaagctgg tgcttgcgat cgcctgcgtc ggacggatgg cgctgaccaa ctatctattg
    25081 caaacgctga tttgtaccac gcttttttac cacctcggtt tgtttatgca ttttgaccgc
    25141 ctggagctgc tggcgtttgt tattccggta tggctggcga atatcctctt ctctgttatc
    25201 tggctgcgtt tcttccgcca ggggccggtg gaatggctct ggcgtcagtt aactttgcgt
    25261 gctgccggac cggcaatatc taaaacatca agataacgat ctggatcaca ttcgttaaca
    25321 aaacggctgt aaccgtttcc attgctgtga ctcgattcac gaagtcctgt attcagtgct
    25381 gacaaaatag ccgccagcaa gcagtcattt actgcaatct cataacaggt agtgaatatg
    25441 atcaccattc gtgatgtagc gcgtcaggct ggcgtctctg tggcaacggt ttcccgggtg
    25501 ctcaataaca gcacgctggt cagtgccgac acgcgtgaag cagtaatgaa agccgtgagt
    25561 gagctggatt atcggccaaa cgccaatgcc caggcgctgg caactcaggt tagcgacacc
    25621 attggcgtgg tggtgatgga cgtttctgat gcgtttttcg gcgcgctggt aaaagcggtg
    25681 gatctagtcg ctcagcagca tcagaaatac gtgctaatcg gcaatagcta tcatgaagcg
    25741 gaaaaagagc gtcacgccat tgaggtgtta attcgccagc gttgtaatgc gttgattgtt
    25801 cactcaaaag cattgagtga cgatgaactg gcgcaattta tggataacat tcccggtatg
    25861 gtgttaatca accgcgttgt gccggggtac gcccatcgtt gcgtttgcct ggataatctc
    25921 agcggtgccc gaatggcgac gcgcatgttg ctgaataacg gtcatcaacg tattggttat
    25981 ctttcttcca gtcacggcat tgaagatgac gccatgcgta aagcaggctg gatgagtgcg
    26041 ttgaaagagc aggatattat tccgccggaa agctggattg gcactggtac gccggacatg
    26101 ccgggcggtg aggcggcgat ggttgaactg ctggggcgca atctacaact taccgctgta
    26161 tttgcttata acgacaatat ggccgctggc gcactgacag cattaaaaga taatggcatt
    26221 gcgattccgt tacatctctc aatcatcggt ttcgatgata ttcccatcgc ccgttacacc
    26281 gacccgcaat taacgaccgt gcgttatccc attgcttcaa tggctaaatt agccaccgaa
    26341 ctggccttgc agggggcagc aggcaatatt gatcctcgtg ccagccactg ttttatgccg
    26401 acgttagtgc gtcgccattc tgtcgcaacg cgccagaatg cggcggcgat cactaactca
    26461 acaaatcagg cgatgtaacc gctttcaatc tgtgagtgat ttcacagtat cttaacaatg
    26521 tgatggctat gattgcaccg ttttaacgtt gtaacccgta tgtaacagtg aataatcact
    26581 ttcaccgagg taatagcgtc ataacaacaa ttaaagccgt tttctggagc gttaccgggc
    26641 atggaagaac gaattttaaa aagtgagctt cggcgttcag taacacttca ttaactctac
    26701 tgccccgccg agcatttatc tcaagcacta ccctgcataa gaaaaaccgg agataccatg
    26761 aataagaagg tgttaaccct gtctgctgtg atggccagca tgttattcgg tgccgctgca
    26821 cacgctgctg atactcgcat tggtgtaaca atctataagt acgacgataa ctttatgtct
    26881 gtagtgcgca aggctattga gcaagatgcg aaagccgcgc cagatgttca gctgctgatg
    26941 aatgattctc agaatgacca gtccaagcag aacgatcaga tcgacgtatt gctggcgaaa
    27001 ggggtgaagg cactggcaat caacctggtt gacccggcag ctgcgggtac ggtgattgag
    27061 aaagcgcgtg ggcaaaacgt gccggtggtt ttcttcaaca aagaaccgtc tcgtaaggcg
    27121 ctggatagct acgacaaagc ctactacgtt ggcactgatt ccaaagagtc cggcattatt
    27181 cagggcgatt tgattgctaa acactgggca gcgaatcagg gttgggatct gaacaaagac
    27241 ggtcagattc aattcgtatt gctgaaaggt gaaccgggcc atccggatgc agaagcacgt
    27301 accacttacg tgattaaaga gctgaacgac aaaggcatta aaactgaaca gttacagtta
    27361 gataccgcta tgtgggatac cgctcaggcg aaagataaga tggacgcctg gctgtctggc
    27421 ccgaacgcca acaaaatcga agtggttatc gccaacaacg atgcgatggc aatgggcgcg
    27481 gtagaagcac tgaaagcaca caacaagtcc agcattccgg tgtttggcgt cgatgcgctg
    27541 ccagaagcgc tggcgctggt gaaatccggt gcactggcgg gcacagtact gaacgatgct
    27601 aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg caaaggtgcg
    27661 gctgatggca ccaactggaa aatcgacaac aaagtggtcc gcgtacctta tgttggcgta
    27721 gataaagaca acctggctga attcagcaag aaataagact gattgggcgc agtcgtttac
    27781 tgcgccctta ttgtatggcc aacaaggtaa aattatatgg tcagctcaac gactccgtct
    27841 tccggggaat acttgttgga aatgagcggt atcaacaagt cttttcctgg tgttaaggca
    27901 cttgataacg ttaatttaaa agtccggcca cattctatcc atgcattaat gggggaaaac
    27961 ggcgcaggaa aatcaacatt attaaaatgc ctgtttggta tttatcaaaa agactccggc
    28021 accattttat tccagggtaa agagatcgat ttccattctg ccaaagaagc actggaaaat
    28081 ggtatttcga tggtacacca ggagttaaac ctggtattac aacgttcggt gatggataac
    28141 atgtggctgg ggcgatatcc caccaaaggc atgtttgtcg atcaggacaa aatgtaccgc
    28201 gaaaccaaag cgatttttga tgaactggat attgatatcg atccgcgtgc gcgcgtcggc
    28261 atattatccg tttcgcaaat gcagatgatc gaaatcgcca aagcgttttc ctataacgcg
    28321 aaaattgtga ttatggatga accgacttct tcgttaacag aaaaagaggt caatcatctg
    28381 ttcactatta ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat ctcgcataaa
    28441 atggaagaaa tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg tcagtggatc
    28501 gccaccgaac cgctggcagg actgacgatg gacaagatca tcgccatgat ggttgggcgt
    28561 tctcttaatc agcgttttcc tgacaaagaa aacaagccgg gcgaagtcat cctcgaggtg
    28621 cgtaacctga cgtcactgcg ccagccgtcg attcgcgatg tctcgtttga tctgcataaa
    28681 ggggagatcc tcggtattgc cgggctggtg ggggcgaaac gtaccgatat tgttgagacg
    28741 ttatttggta ttcgcgagaa atcggctggc accataacgt tgcacggcaa acagatcaat
    28801 aaccataatg ccaacgaagc cataaaccac ggatttgcac tggtaactga ggagcgccgc
    28861 tcaacgggaa tttatgccta tctggatatt ggttttaact cgttaatttc caatattcgc
    28921 aactacaaaa ataaagttgg tttactggat aactcgcgga tgaaaagcga tacccagtgg
    28981 gtgattgatt cgatgcgggt aaaaacgccg ggtcatcgga cgcaaattgg ttcgctctcc
    29041 ggtggtaatc agcaaaaggt gattattggt cgctggctac taacgcaacc agaaatatta
    29101 atgctcgatg aaccgacgcg cggtattgat gtcggggcga agtttgaaat ttatcagtta
    29161 attgccgaac tggcgaagaa aggcaagggg attattatta tctcctctga aatgcctgag
    29221 ttgttaggga taacagaccg tattctggtc atgagcaatg gtctcgtttc cggaattgtc
    29281 gatacaaaaa caacaacgca aaacgaaatt ctgcgtcttg cgtctttgca cctttaagat
    29341 taggggcttc ccatgagtgc gttaaataag aaaagttttc ttacttacct gaaagagggc
    29401 ggtatttacg tcgttctttt agttttgctg gcgattatta ttttccagga cccaacattt
    29461 ttaagtctgt tgaacttaag taatattctc acccagtcat cggtgcgtat tattatcgcg
    29521 ctcggtgtgg cagggttaat tgtcacccag gggaccgatc tttctgctgg tcgtcaggta
    29581 gggctggcgg cagtggtggc tgcgacatta ttgcagtcca tggataacgc caacaaagtg
    29641 ttcccggaaa tggcgacgat gccgattgcg ctggttattc tgattgtctg tgccattggt
    29701 gcggtgatcg gtttgatcaa cggtctgatt atcgcttatc tcaacgtgac gccgttcatt
    29761 accacgctcg gcacgatgat catcgtctat ggcatcaact cgctctatta cgactttgtc
    29821 ggggcgtcgc caatttctgg ttttgacagt ggcttctcta cctttgctca gggctttgtc
    29881 gcgctgggga gtttccgtct ctcttacatc accttctacg cgttgattgc ggtggcgttc
    29941 gtctgggtgt tgtggaacaa aacccgcttc ggtaagaaca tttttgccat tggcggtaac
    30001 ccggaagcgg caaaagtatc tggtgtcaac gtcggcctga acctgctgat gatctacgcg
    30061 ttgtctggcg tgttctatgc ctttggcggg atgttagaag ccggacgtat cggctctgcc
    30121 accaacaacc tcggctttat gtatgagctg gatgctatcg cggcgtgcgt ggtaggcggt
    30181 gtatcgttca gcggcggtgt ggggacggtg attggcgtgg tgaccggggt aattattttt
    30241 accgtcatca actatggcct gacgtatatc ggcgtaaacc catactggca gtacatcatc
    30301 aaaggggcga ttattatctt cgccgtagcg ctggattcac tgaaatacgc gcgtaagaag
    30361 tgatgtgatt gtggatattg ccggatgcgc tgcatccggc aatcggtgcc aaatgccgga
    30421 tgcgtctctg gttttacaac gttaccgggt gttctttctc gcctttctta aacttcactt
    30481 ccccgagctc aatacaaccc accgggcaga cgtgaccaca aagcagacaa cccacacatt
    30541 tctcggtatt acaatgcggt gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc
    30601 cgtcgtaaca ggaaatataa cagcgtccac agccaacaca tttatcaaga ttgatacggg
    30661 gatagacaat ataactgcgg tctaaatctt ccgccgggac aatattgtta ttcgccagac
    30721 ctaccatctc ctgcagcgaa tcaaatccct gatcggcgag ataatgcgac aacccgcttg
    30781 ccatatcttc cactatccga tacccgtact gcatgatgcc ggtggtcacc tgtaacgttg
    30841 ctgcgccgag caataaaaac tcagccgcat cctcccaggt ttcaatgccg ccgataccgc
    30901 tgattgggaa atcgcgcagt tctggatggg tgcgcatttg ctgaatgaag cgcagggcga
    30961 tcggttttac cgctttaccg gaatatccgg aaatactcga ttttccgtta acgatcggca
    31021 taccgatttt ctgattaaga tcgatattgg tgatggattt aacggtgtta atcgccgcaa
    31081 tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc gcacatatca ccgatattcg
    31141 gcgtcatctt cgccagcatt ggcagcgtgg aaccccgttt cactgcccga caatattttt
    31201 ctaccagctc cgggctttgc ccgacatcgc tacccatcgc atgagaagtc atttgcggac
    31261 aggagaagtt acactcgatc atatccgcgc cagcttcttg caccaggcgc gccagctcct
    31321 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    31381 ccttcagccg acgcagggcg gccagattct cttccaacgg atgttcagca atctgctcca
    31441 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    31501 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    31561 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    31621 tgggaaactt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    31681 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    31741 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcgggtcgc gggtctggta
    31801 attttgtgtt ttgatggtgt tacgctgcgg ctctaactcc gcaaaggcat ctagtctggc
    31861 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ccgataaccg
    31921 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    31981 cgaaacgccc agctcccggg cgctggtaaa ctctttttcg cttgccggaa actcatctaa
    32041 ctcttcacgc gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    32101 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    32161 ttgcgcctgt cgtgcacgtt gcaagaagtc gacggcaatc tcaacgtcac tatgctcaaa
    32221 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttctgc
    32281 tttcagctgc tcaagggtga gtgtgttacc gacttcgttg ttgcacttaa tggtcacgcc
    32341 cattttttca atacgggcga tctctgcatc cagtactgat tgcggtaacc ggaattgcgg
    32401 aatgccgtta cgcaaccagc caccgggttg tgcttctttc tcatagatcg tgacgtcata
    32461 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    32521 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    32581 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    32641 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    32701 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    32761 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    32821 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    32881 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta aggcgaacgc
    32941 taaatatact cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct caccc
//
LOCUS       DACAPB010000030        32976 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.030, whole genome shotgun sequence.
ACCESSION   DACAPB010000030 DACAPB010000000
VERSION     DACAPB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.030"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(18..779)
                     /gene="ydeO"
                     /locus_tag="IT904_001270"
     CDS             complement(18..779)
                     /gene="ydeO"
                     /locus_tag="IT904_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAP3191081.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            complement(854..1051)
                     /gene="safA"
                     /locus_tag="IT904_001271"
     CDS             complement(854..1051)
                     /gene="safA"
                     /locus_tag="IT904_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAP3191082.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQG"
     gene            complement(1299..3578)
                     /gene="ydeP"
                     /locus_tag="IT904_001272"
     CDS             complement(1299..3578)
                     /gene="ydeP"
                     /locus_tag="IT904_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAP3191083.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(3912..4826)
                     /locus_tag="IT904_001273"
     CDS             complement(3912..4826)
                     /locus_tag="IT904_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3191084.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDAIKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(4885..5388)
                     /locus_tag="IT904_001274"
     CDS             complement(4885..5388)
                     /locus_tag="IT904_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3191085.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(5401..5931)
                     /locus_tag="IT904_001275"
     CDS             complement(5401..5931)
                     /locus_tag="IT904_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3191086.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(5945..7171)
                     /locus_tag="IT904_001276"
                     /pseudo
     CDS             complement(5945..7171)
                     /locus_tag="IT904_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            complement(7426..7634)
                     /locus_tag="IT904_001277"
                     /pseudo
     CDS             complement(7426..7634)
                     /locus_tag="IT904_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7946..9268)
                     /gene="hipA"
                     /locus_tag="IT904_001278"
     CDS             complement(7946..9268)
                     /gene="hipA"
                     /locus_tag="IT904_001278"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HAP3191087.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            complement(9268..9534)
                     /gene="hipB"
                     /locus_tag="IT904_001279"
     CDS             complement(9268..9534)
                     /gene="hipB"
                     /locus_tag="IT904_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HAP3191088.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            complement(9757..11157)
                     /locus_tag="IT904_001280"
     CDS             complement(9757..11157)
                     /locus_tag="IT904_001280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP3191089.1"
                     /translation="MNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVK
                     GDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGK
                     GNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFS
                     HRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDL
                     AQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKT
                     GAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWY
                     VQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQ
                     PYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGD
                     KGYSDTQGMLGVKYSW"
     gene            complement(11154..15177)
                     /locus_tag="IT904_001281"
                     /pseudo
     CDS             complement(11154..15177)
                     /locus_tag="IT904_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(15708..17300)
                     /gene="lsrK"
                     /locus_tag="IT904_001282"
     CDS             complement(15708..17300)
                     /gene="lsrK"
                     /locus_tag="IT904_001282"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HAP3191090.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLCADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNVP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPAPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(17379..18332)
                     /gene="lsrR"
                     /locus_tag="IT904_001283"
     CDS             complement(17379..18332)
                     /gene="lsrR"
                     /locus_tag="IT904_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAP3191091.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            18581..20116
                     /gene="lsrA"
                     /locus_tag="IT904_001284"
     CDS             18581..20116
                     /gene="lsrA"
                     /locus_tag="IT904_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAP3191092.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRTMLNGNEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            20110..21138
                     /gene="lsrC"
                     /locus_tag="IT904_001285"
     CDS             20110..21138
                     /gene="lsrC"
                     /locus_tag="IT904_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAP3191093.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVTTLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIERLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            21138..22130
                     /gene="lsrD"
                     /locus_tag="IT904_001286"
     CDS             21138..22130
                     /gene="lsrD"
                     /locus_tag="IT904_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAP3191094.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            22142..23164
                     /gene="lsrB"
                     /locus_tag="IT904_001287"
     CDS             22142..23164
                     /gene="lsrB"
                     /locus_tag="IT904_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HAP3191095.1"
                     /translation="MTLHRFKKIALLSVLGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            23191..24066
                     /gene="lsrF"
                     /locus_tag="IT904_001288"
     CDS             23191..24066
                     /gene="lsrF"
                     /locus_tag="IT904_001288"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAP3191096.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            24090..24380
                     /gene="lsrG"
                     /locus_tag="IT904_001289"
     CDS             24090..24380
                     /gene="lsrG"
                     /locus_tag="IT904_001289"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAP3191097.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            24437..25195
                     /gene="tam"
                     /locus_tag="IT904_001290"
     CDS             24437..25195
                     /gene="tam"
                     /locus_tag="IT904_001290"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAP3191098.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(25199..26113)
                     /locus_tag="IT904_001291"
     CDS             complement(25199..26113)
                     /locus_tag="IT904_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191099.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(26320..27771)
                     /locus_tag="IT904_001292"
     CDS             complement(26320..27771)
                     /locus_tag="IT904_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP3191100.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(27998..28945)
                     /locus_tag="IT904_001293"
     CDS             complement(27998..28945)
                     /locus_tag="IT904_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP3191101.1"
                     /translation="MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALI
                     VSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISV
                     GSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGL
                     YEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLV
                     ARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASY
                     RTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(29057..29416)
                     /locus_tag="IT904_001294"
     CDS             complement(29057..29416)
                     /locus_tag="IT904_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAP3191102.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAAALPVNDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(29416..30342)
                     /gene="glsB"
                     /locus_tag="IT904_001295"
     CDS             complement(29416..30342)
                     /gene="glsB"
                     /locus_tag="IT904_001295"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAP3191103.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(30406..31794)
                     /gene="sad"
                     /locus_tag="IT904_001296"
     CDS             complement(30406..31794)
                     /gene="sad"
                     /locus_tag="IT904_001296"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAP3191104.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARHMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            31895..32776
                     /locus_tag="IT904_001297"
     CDS             31895..32776
                     /locus_tag="IT904_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191105.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVIAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLEVPDSARQGYQ"
     gene            32854..>32976
                     /gene="yneK"
                     /locus_tag="IT904_001298"
     CDS             32854..>32976
                     /gene="yneK"
                     /locus_tag="IT904_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAP3191106.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTII"
BASE COUNT         8296 a         8359 c         7685 g         8636 t
ORIGIN      
        1 tactcgttag caaataatca aatagctaaa gcactcatcg tgttgcccgt attcataccc
       61 gtgtgacttt gacaacggta ctccttagaa actctcttcg gactgttgcc gaaatgtttg
      121 cggaacgcat aaataaaata agatgtactg gcataaccac attgttcggc aattttattg
      181 actgaacctt ctacgcgtat caaatttttt gcgtgctgca ttctggcatc taatagaatc
      241 tgtgagaatg tcgtttgctc ttgctttagt tttttcttca acaggctttc actgatgtac
      301 aggcagtcac aaatatcttt cagcttccat gggtgcgcca gcttcatgtt gacaatattt
      361 ctcacttttc cggaaacgga tagcacaccg ttagttaata gtgtaatgaa acctttgcat
      421 gcggcaaaaa tagacaggca agaaaaaagc aaaagttctg aaaaattatg atgattgcgt
      481 tcttccgagt tgagataagc aatcatttca ttaagcaatc cagtcggtac atttgaagca
      541 cgtaaaaact tcgcaggaac aggcaccgtc actgtctgga tgttagtgca ttgtagatat
      601 tttttaatgg tattttcagt aaatccgatc tctttaaaat gccgctcata aggttcaaaa
      661 tgcgtacgaa cagcgttatc taccatcagg atctcgccgt cagagaaggc gtaatcttta
      721 tctaaaatgt tagcgttgaa ggcatgatga ataaatataa cagaacaaac gagcgacatt
      781 ttatctcctt aaaacaataa agtttttatc gatacttttt taacatttca tatttataat
      841 ttgctgtttg ttttcagcct tgcaaactat tgataatgaa atgtgtaaga tctcttgcaa
      901 tgcgacccat ttctgaaata ctcagctgat caattgccga aaaaatggct atcaacgtaa
      961 gtgtcagtaa taagacaacc acaattgcag acatgatttc tttatagttg tctctttgcg
     1021 tgattttgtt tttcactgtg gtcgcatgca tttcaaatat gtttatttag cggataacgt
     1081 taaaaatcgt taatcagtat gcttaatata acaagctgga gaaaagagga aataggactg
     1141 gtgcttcagt actgagcgga gtttcttaca gctgtaggca gaagtttata tcttatagta
     1201 tgtagtttaa tttaatccat gtgagcaaat tgcgaaataa tagtcaatga ggaattcttc
     1261 tgctgtgcgg atgacagcag aagaaatgag aagaggcatt aatttgatgg ttctaattca
     1321 accggaatac ttttatagcc aggaatgcca cttaatggat cgtggttatc aagtgttagc
     1381 atgtgattcg attctggaaa ataggtcacc agtgagcggt cagccatagg gtaaatgacc
     1441 acttttaatc tatccatgcg gcgtgagctg cgcttaccgt ctggcgtaag cgcaataaga
     1501 ttaactcttt cgccgttttt tacacggcaa attttagctt gtttagcact cataaagacc
     1561 acatctcgtt gaccgaatac ccctcgatag cgatcatcca taccataaat cgtcgtgttg
     1621 tactgatcgt ggctgcgtac tgtcgccatg accagcttac tgttaaacgc tgaagatgga
     1681 tcttctaaca gccctttgct ggtaatgaaa ttagccttac ctgacggcgt catccagcgc
     1741 ctttcagcag ctgcatttat caggtgaaaa ccaccgggat gacggatgcg ctggttatag
     1801 tcggcgaact ctggcagcac agcttcaatg tcattgcgaa tgcgatcata atcttccacc
     1861 agatactccc aggctaccac gctctggggt agtgctgcct gcgcgattcc cgcgaccact
     1921 gcacactctg atttcagcat tacaccggcg ggttttaaca cgccacgcga ggcatgaatc
     1981 atcgacattg aatcctcaac ggttaccgcc tgcgcaccgt ttttttgcat gtcaatctcg
     2041 ctacgtccca ggaccggcag aatatagcta tgccgtgcgg tcaacagatg agagcggtta
     2101 agcttagtgg ctacgtgtac cgccaaatct aattgcgtta acggtacagc gctcgcttcc
     2161 cgatctggca ttgccagcgc aaaattgccc cccatgcaga tcaatgctcg agcctgcccc
     2221 gtacatattg cttgcatgct ggcaattgca gcatgtccag gtgcatgagg tggggtgaag
     2281 ccatagcgct cacccagacg agccagaaac tctgcagacg gtttctcggt gataccgacg
     2341 gttcggtcgc cctgtacatt agagtgtcca cgtagtgggc agatacccgc accaggcttg
     2401 ccaatgttac ctttcatcaa cagcagattg accagttgct gtacgttctg ggtaccatgt
     2461 tcgtgctgag tgatccccat tccgtaacag ataatggtgc gttcggcagc ggcatatgcg
     2521 tcagccagtt cggcgatttg tgtctgactt agtccagaaa tacgttcgat atctttccac
     2581 tcggaattga gaacgtcacg gcgtagctcg tcaaagccga cggtatgcgt ttgaataaac
     2641 tcgtcatcaa gcaatgaagg ccgacctgcg gcgcttgcag catcatcgcg ctcaattaac
     2701 aggcgcatca tccccttgag caacgccata tcgccaccaa tgcgcacgtt atagtaggca
     2761 ctggccaact gagtctcaga gttcgtcagc atttcaaacg ggttttgcgg tgcggtaaat
     2821 cgctccaggc cacgttcctg tagaggattg atggcgatca ttttcgctcc ccgtttcact
     2881 aaagcgcgca acgaagtcag catgcgaggg tggtttgtac cagggttatg cccaatgcaa
     2941 atgactaaat cgcacttctc aaagtcttcc agcaacacgg tccctttacc tacaccgata
     3001 ctcgctgcca aacccacgct tgtcggttca tggcacatgt tggagcagtc gggaaagtta
     3061 ttgctcccgt attcacgggc aaaaagctga taaagaaagg cagcttcatt ggaagtgcgg
     3121 cccgaagtat agaattcaac ctgattggga tcactatagc tttgaaggcg tgcgccaatt
     3181 tcgtcgaaag cttgttgcca gcttaatggc ttgtaacagt cgctgacggc atcatatttc
     3241 aaaggctgag tgagtcgccc cgcagcctca agctcgtggt ctccccaggt aagtaatgat
     3301 tgaaccgtat tctcagcaaa gaaagaggcg tttacctgct tatccgtgac ttcccaggcg
     3361 attgcttttg cgccgttttc acaaatgtcg aatgacgcac tgtgcttagg atctggccat
     3421 gcacaacccg gacagtcaaa gccctctggc ttattcatgt caaacatggc aataacatcc
     3481 tggcgtatat ccatctgctt acgtactgca ttcgctacgg atttaacagc accccaacca
     3541 cctgcagcac cctggtagga ttcaattttt ttcttcatct acttatcctg tgtggaaatg
     3601 aattattagg atttgtaata gcgatgatgc acagtacata caaagggatt atcgccaaca
     3661 tggatatata agagtagggg actactctct ttctattttt aatctattat cgttaaacaa
     3721 tagattaaat acgataagtt aagcatcatc tctcatagtg tttaaattaa catatttttc
     3781 aatataaaaa ataagactct ggcttcaatt gtgcgcggat tttcttacag gtgtaggcta
     3841 atagccttca caaaataatt atccgcgcaa ttttcgtgaa tggagcgcat agactaaggt
     3901 caaaatcaca attattcata gataaaagtg acaccaatga ctgactggac ggttccggct
     3961 gaaaccttgt ttcctgtttg tccataggtt gcgcttaaac ccagaggtac tttgctttta
     4021 tttactgtcc ccagggagac attttctccc gtagccagta tttttccatt acgcattaga
     4081 gtgacaccca ctcccgatgc ttttattgca tcaggtgctg tgttagcaaa cacttgtcgg
     4141 ctactgtcag tggtggcacc cgaaagataa aatgagagct tttgttcgct tgagcaatat
     4201 acgccaaggg gtatttctgc gcttccagga aagtccggga gatccacggt gacattacga
     4261 ctatcaacgg tgcagccgcc tgttggcata acaacattgt tatttgaaat aatattccac
     4321 gtaaaattac gcggatttcc gcttcctaat gttgcaattt tatacatatg aatacgggca
     4381 atgacttccc ccgcttttat gacaactcca ccggccgccc caactggggt aatatagagt
     4441 tttaacggta atggcatcgg tgtcttatca ccaatatcca atacgttggt atttgtcgtc
     4501 aacgggaagg ggtaggtcac attattccag tacagcgatc ctttatatga ctgtagggag
     4561 cctgcgaaag ctgagccttg taccaggttt atatgatcag tgtcgtacca gccgccgtaa
     4621 tcattccagc aacttatatg ctgagacaag tctacaacca gattctggcc cggctgtatt
     4681 acagggtcga gattaacata aacagaggtt gtacctgctc caatacttga cccgccatcg
     4741 acattacaac tgaaggcaaa aactttgcct gccattaata gaagataaat accgaacagg
     4801 acctttatac tgattgtttt acccatgata tatcctaagg ttaaaaattg atttaaaaag
     4861 aaggctaaga aaatcgaatc gacgttattg ccaggtgtag atcacattga ttagcgcctc
     4921 gatcgttccc tggcttgcgt ttccattcac cgtgatagct cttgccttaa gtggaaactg
     4981 tgcattacga gtgatctcat caacaataac cgttttgcta tcgccatttt ttaacgcagc
     5041 atcctggtca tccctcagct ctatctgaat attttcggca gtaccttcat ttttgtaata
     5101 acccgtattg tcagttgaac ctgtcacgat tgccgtcact gcacttgttt caaccggaca
     5161 atcggttaat gacaaagtaa tattgtgcca gccagatgca gaaccaggtt gttgcagatt
     5221 gcgcgtataa agatccccga gattaacgtt agcttctttg gtttgaatag tgcagggttt
     5281 agcgactacc cgaccattaa cagtgatagt gacatcggcg gcctggagag ttgctgtgaa
     5341 taacgcgcaa aacgtagcta acaggaacct cttgtgaagt cttttcatat ctgactctga
     5401 ttattgaaat tcaagggtaa aggttgccga agccctcacc agtcccggat tgacggactt
     5461 ctgagttgac ttcagacgag cggagtaagg caaaatattg ttctgttctg gtaccagtgg
     5521 gatccactgc atcccggcat gaagatcatt cagtttcacc ggacgcatat ttgcgtccaa
     5581 tatttctata cccaaaccgg aggccgtatt gcttccttca tccagtttca acaaagtatt
     5641 attttctgca tcctcaatac cgttaaatgc aacccgaacc cccgttgtcc ctttgctgca
     5701 ttcacttaac gtaatctgaa aagggacggc tggacttgtg ctaccggtcg ttggaaattg
     5761 tctggcactg tttttttgga gatctacggt aaaattaagc gaatccgatg agactgtgca
     5821 gccataatcg aggacgcgcc cgctaatttt aataacgcta tctgcggata aagcagaata
     5881 ggtggttaac cccagacata aaccgaggaa aataatgtta ttgtatttca taatctattg
     5941 ttccttagcg acagattgct gtctgctggt tcagtaaggt accaggagaa acttcaggaa
     6001 gcttgtactc gacaatacag tttgagtttt tatctttgcc ccatgaaacc tgtaattgcc
     6061 ctgactgtgg aagtccagtc agataaacct gaccattttc cgcgacaatg ctgccatttt
     6121 tattctctcc gtgtgtgaca attgcaccga atggaacgct cttattaccg tacttcaacg
     6181 tcattaatac tttcccgccg atttgtgcat taaatgttgc tctggcaata gcaccgtgag
     6241 ttgggatgac agtgaccacg gtttcatcca gttcaacatt atctgcaagg gaattcgcgt
     6301 taagagcaac acggttttct ctatattctg tcgcaaatgg taatatggca tagccacgcc
     6361 agtcggtatg aattccggtc tggttctcta ttttgacatt atcagcacca ggagccttaa
     6421 ccagaaccat tgtgtcgccc agcggctgtc caaaggtgat gccatcagca tgagcaataa
     6481 tcccaccact cattccgtaa taaatctggc tgctgtcacc actccgactg taaccgacat
     6541 tagtattacc ataagctcca cgataattaa gagaactgta accactggtg ccagacgatg
     6601 tattacctcc gtgggtgtta ccgacctgaa cgctataatt caggttatta tccggcagca
     6661 gagtgccata aacccccgat agattggtca tgccgccttt caaatcgttt gacatactgt
     6721 aactggcgtt tgaattacga aataccgact gactgtctgt acgcatccaa tgactgaagg
     6781 gaacattaag cgtaaaagcg agtaaatgat cccgatcgtt ttgccatata ttattggaat
     6841 agctgtaatt cagcgaagtc gtaatatcac caaacggcac atttaatcca aatgatattt
     6901 gctggtcgct gcgtgacgtg ttccagtaac tttggcgact ggcactgaaa aatgtcgtac
     6961 cgtaatttcc aagctgctga gagatgctta tttgttcctg accacgctta ctgtagaaca
     7021 gattaaaata atcaataaat tgtgtctgct cattggtgtc tccggtagga ggcttgacgg
     7081 tgtaaccact cattcgactg taggcactgt cggataagtt ataaaaacct tgcgtagaat
     7141 agcgatatcc tgcgacctgg atattggttc cacaacaagt ttattgctgc acttgttgaa
     7201 gagagtattt gcaaaaacat tttatgtcgc taaataacat gacgatttca ttatgcatgt
     7261 tatttataaa ccgtgactaa acagaatatt attgcgaaca agggaagtgt caggaaaggt
     7321 caagagctac caatggttaa tatggctata aatcattagc tatggttaat gtttgcttgc
     7381 tgaaactaac accgaattgc tctaaacaga taagtgcagg gtttattaaa gctgtccgac
     7441 agactttctt aacctggtaa tatctctctt gggtgactct ccaaacatcc gcgaatattc
     7501 ccgactgaaa tgggatagct ttcgtaaccg acagcatagg ctgctgtggt gacatcgtaa
     7561 tgttcattca gcatcggtcg cctgacttca ttgagacgta gccatttctg ataccggagt
     7621 ggactcattc ccgcttgatc cgtccttaaa taagtaacag caccctctgg tgttttcgta
     7681 tgcagtgtct atgcgacaga cactgcgctc ttagcatggg gcagggaata accaggctat
     7741 ttctccatgc acaggccaat tatgaaagag gagaggctta agttcttgaa ccctgaacga
     7801 ataacgacta ccagaaaagc acttcgcatc actcagacat gatttaacat aatatacatt
     7861 atgcgcacca atataaacca aggaagaatc cagtcgttgg cggtcatgat tgtcatgctc
     7921 attaacaatg accaaacccc atatctcact tactaccgta ttctcggctt aaccgtccat
     7981 gcaacctcaa cacattgctt tcaactgccg tcaccacgtt ctccggaaaa tctgtcggta
     8041 atgaagtctt cacgttatcc agtgctgctg gaatcattct ggcaaagtca ctcaggattt
     8101 catgcatctg cacttccggg aatctcagca cctttgctgt cgccaaaaaa tgtcgcggat
     8161 aaattttatc gattgccgtt tttttgcctt tggatgcgtt aagccccatt gccagtttga
     8221 gatcgctgat gtgtattccc gtaccgccaa ggaccggaaa tgctgaaatg atgtcgtaaa
     8281 atggcgtgag tcgataactg ccgccagcct gaataaatac ggagaagttt tttgcatgac
     8341 cgtccgttgc gccaatcaac cactggaaga cctggaattt cataaaatca tagcgatctt
     8401 tcagcgcctc gctggacccc atcaaaaaag ccatgatccg cgcgatgcct gggcctccat
     8461 ctgattcgta tttcaccgat gaaggtaaac cgaatgtctg acacatatcc tcctgtggca
     8521 agcgaagtaa aaccgttcgc tcagcattcc aacgcctgtc aaaacgttcg accgctaacg
     8581 cgcgcacatt tcccgcttta atgatttctg cgtccggaac attcaaccca agttctttcg
     8641 ccagcagcag acagtaatac tcattatcaa cgctttggct gagatcgagc gtcgcattgg
     8701 gctgcctgat ttcgccaatc ggtaatttaa tgatgtgcgt cgtcggcgtt attcctttcg
     8761 gaatgcacca gtcattgcct attctgagca gtgctgtctt ctcctgtgcg ccagcaaccg
     8821 agatgcgaaa gtcattttct tctctaatca tgcctagcgg gatatctgct ttataagccg
     8881 ttaatacttc ttcaagtctg gcttcagtaa gcttttccca tgccattatc ggatgcgtta
     8941 cggtttcgtc ttcgggtatt aacgtcacgg caccaacgct gtctcgccct atttctgaca
     9001 ataaatcaaa cggttgtctg gatttggcat gataacgttt aacgatccgg tcacgtacaa
     9061 tcgggctatc gggtaacagg ttatcgaaga agttaaatac ggcatcagag gtgatattcc
     9121 ccctctgcaa tggcagcgaa agtgacaacg gtctggcata acggcttgct aaccactccg
     9181 gtgcatactt aaaggtgtgc gcgccgttgg ctaacttcgt taactcgcct acccgctggt
     9241 tgttcatcca agtgacaagt ttaggcatta ccactccaga ttttgctgtt ctgttgattc
     9301 tggcgaggca tttttcgcgt cgcatagcgt cattgagagt tcaagcgact gtaaaatctt
     9361 aaaaaatgtc gtgagcgtgg tattgtcagg gttgttttcg aaattggaaa tcgtcgcctg
     9421 cttaatacca atttttttcg ccagctcgct ctgcgtccag ccattttgct ggcgaaccag
     9481 tttcattgca ttcgccaatt gcgttgggct atagatcttc tgaaagctca tcatgtcata
     9541 cgtccacagc aagtttatcc gcttaagggg atattataag ttttatcctt tagtgaggat
     9601 aagtcaatct ggaaacacat cttatccgct ctacgggata aacgctgcga tatccgcgat
     9661 cgcggataaa aaggaggatt taaataaggc gttgatgcgc cgtttgaagt cggcaatatt
     9721 aagccgcatg ccatctcgac atgcggctta tacggtttac cagctatatt tcactcccag
     9781 catgccctga gtatcgctat agcctttatc acctagttgc acaccgacat tcccccacag
     9841 gctaaggttg ttatttactt tcgcctcaac cccggtacgt acttcaccga gattacgcgc
     9901 accttcacgg cctacggttt gaccattcat cttcacggcg tagactttgc tattgttgat
     9961 ccagttcgct tcaatgtaag gctggaactc acgctgttta ccatcgtcac gctggtgatg
    10021 gctattcagg taggttttca ccccaagtcg cgtttgcaca tttccgtcgc cttccgtttc
    10081 aatgcgcgtt ccgtctttcc gggtatggtc ggaatctttc acacccatcc aggtgatttg
    10141 cgcctgtggc tggacgtacc aggtattcag cgtcccttcg ctgccgctaa atgttcccgc
    10201 ttcaaaggta tacccaccct caacagaggc cgtcacaccg cgagaatcat agtcgtcagc
    10261 agaacggtta tcggaactga cgctgttatc aaaccagtta tacagcgccc agctgtcaac
    10321 ataagcgccg gtcttattcg catcgttctg ataccaggtc gcgtacagcc cagcgctgta
    10381 accgctgatg cgcccatccg atttataacc cacacgatta ctctgagtat tactgtgctg
    10441 attggcgtag cctgccatca cgccaagatg ccagcgatcc tgcgcgttgc tactccactg
    10501 cgccaaatcg ccgcctagct gcaatacata gcggttagcc tgagtattta gctgaccgtc
    10561 accggccctt gaacgttcgt gccctccgac atgacgcatc cacatactgc ttgccgaccc
    10621 ctgagaatgc agtgaatctg tatactgcgg ctcacccaga cggtcgtgta aacgatggct
    10681 aaacagcgag ttggctgcgg caatgttgct gatatagctt ccggcctccg ggcgataaac
    10741 tgatgggcct tccggatcca caacaggggg attattgatg ggatcgggtg tatccgctgg
    10801 cgttacgccg tcccatttac tggtcagata ccagtttttc tcgtcattcc ccttcccttt
    10861 agccagcgtg tagacgtaag ccccagcttc gacagttccg gtggtcagcg cgaagttacc
    10921 tgcagaatta ccgccaacct caatgagttc aataccgttg accgtttgtg caccgacgcc
    10981 gccaatgtta tcaacccgaa cgcgagtgtt cccttgggta tcgcctttca cgttcatctt
    11041 atcggtgggc gaattatcgc cgcccagcgt cgcgttgaac gtcatcaggc cattgtttcc
    11101 ggtatagttg ccgttaacgg tcagtacatt ccccggtttc cccccttcgc tattcatttg
    11161 aaccgtgccg ccattctcca ggttgccgct gatagtggtt ttagcgacac gcagtgcccc
    11221 cccaggcata acgttcatat cacctgcagt ggtgacattt cccgacatca caccgctgtt
    11281 atggatatta atgtgttgac ttgccatatt aatggcagag tcggaacgga aagcaatttc
    11341 cccccccacg atatccgtcg taccctgata cgccgcggaa tcgttgttaa gagtcaggat
    11401 cccggtaccc agttttgtca catcaccgct accgctgata ctgtgcgtca tcgcccagct
    11461 acgctcagtg gagatggtga gcaggccatt attgataacg gaagcatcac ccaggttttt
    11521 ctgctcgttg acgctgagcg cgctgcctgt atcaatgttg aattgcccca caaagcggct
    11581 attatcgcca tccagctcta catctgtcct ggcggttgcg ctcacgatac ccttcccgct
    11641 tatgctattg cgcagttcgc cagtcacgtt ttttagcgtt aacagaccgt cattggcaat
    11701 atttcctctg cctaacccct gcgtattatc gaggctgact tccgcatttg ggctaatgct
    11761 ggttaacgca ttgtagcgtg cattaaaccc ctcgatatcc agcgttcccc ctgtaacatt
    11821 caggtttccg ccacctgtca gttcaccctg actgatccca cctttattta ccgtcagcgc
    11881 cccctctttg aacaaaacag tcgaacccat ctgcccggtg aatatctcta ccgtctgcgt
    11941 cgatccatta agatcgacga tggccgcgtt gctgatgttc agttcccggg tgttgccaag
    12001 tgcaccatcc gcatcggtac gtaatgtccc catctgaacg taggttgccc cctgatagtc
    12061 gttctgaccg ttggaaagcg aaacctgtcg caccgtattg attgccagat ccccttcacc
    12121 accgattttt gccgacatat cggccgttgc gccataggct ccgccatgtt cggctaacgt
    12181 cagcttttgc ccaccatgga tgttcagcgc tttcagccca tagttaacgt acaaaccatc
    12241 acctggtgcg gtcgtgaggc ggaatccata atcaccctcg gcgaccaccg ttccgttttg
    12301 cgcaatgtgt aacgttttgt ccgcttccac ctcgtcgccc tgtaaatcac gtaacgttaa
    12361 tgagccaccc gacccaataa ccgtttgcgc cttcaccagt tgaacgccgt catggctatc
    12421 atcgtgttcc agcaaattga gcgtcgtcag agggttattc gccatgggat cattccacgg
    12481 tttaggcacg tcgataagca cgtcgcccgt cccgtttacc tgatagttac ggcctttcca
    12541 ggtgtagtca cccgcgccga caaccagcgt atcgacgctg atatatccct ctgcaagcgt
    12601 cgcagcagga atatccgtat cgaaaatgag cgtaccgcca ttcatggcca gtccaccaat
    12661 ggattgctct cccacgttta ccgatgtggt attttcactg tcagactgca acatcgcgtg
    12721 agtaagcgca gcggtgttgt cgcgttccag agtgaaggta ctgtctttca gttgggcaac
    12781 accggcgaat tcagtccctg ttgcatgggt aaagccaaat atcttgtcgg aggatgacag
    12841 ctgcactcgc atcaaaccat cgcctttgag cgcattggtc agcttgtaat ctcctgcact
    12901 gttcgttgat gcgagaatgt cgagcgtacc ggggcttgca atggtgactt cactcgaacc
    12961 catgcccgtt accccatctg ccgtatgaga cgcaatggtc aggaggccgt cattgaccag
    13021 cgttttaccg ctataagtat tccctgcatt gaacaccgta tggcctgcat catggatcac
    13081 ttttccgtct ttatcgtcac ccgtaatcag catatcgacc tggtagccgg catcactgtt
    13141 attagtatga ttaaagacaa aaacaccttc gccagaacca aactctactt tcgtcgcgtt
    13201 ggttatatac ccggcatctg ccgccgcctc accgtgagct gcaccaatgt ttagcgttcc
    13261 tacagcaggt tcaaaaacac ctaagtaaat ttctccacct tcgacattca gcgtgccgct
    13321 attcgtcagg gttaacgtac ccgtaccaga tgtccctacg tacatattga atgtttcaag
    13381 aagagagttc tgcccatcca ccctcacgtc gcccttactt ttgtcattga gagctatctg
    13441 tgtatcacgc gctttaacta tacctccggt ggtaatattc agttcacccg tgcctaatac
    13501 accgacttgt agcaattggg cgttagtaga agacgtcttt aaattccaga gactatccgt
    13561 tgaaatatta actattccct taccggaggc atttcctccg ataaatccat agcccgttga
    13621 attaagggtg gcgccattac tgacatttac gatgccgcta ccaaaattac cgacattgat
    13681 acccacagta tcgaacgatg atgtcgcatc catcaggttc aatgtgccga taccgcttgc
    13741 tttatcaccc caaaaagtaa tattttgcga tacgaactta ccgccatcgc taatattgag
    13801 gtttccgacg cccgcaacac caatggtggt atagacattc ttattgacct cccacattcc
    13861 ccccgtggtg atgctgacat tcccgacgcc ggtttctcca taaccgagtg aacttcctcc
    13921 gttgctgaca acaagcccct gattactgat attcatttcg ccgtggccgt cataaccaag
    13981 attagttccg agattagtga taacggagtt cttatcctta acagtaatgt tgcctgtgcc
    14041 tgtttctttc atccccgcag tgataattcc cgaatctact ttgccttcac tcgagacatt
    14101 aagttcaccg tcgccagcat caccgatata gatatagcgg aaagcttcgc ccgttccgag
    14161 gaaattccaa tgccctttat cggtcacgtt gacgacaccg tgggaaccgt cctgaacgcc
    14221 caccaatgaa tattctttgt tattaatcag tccattattg gaaatattga ctgtgccatt
    14281 accgaaatat ccattataga gtctgcgtac cgtgatgaca gagtcttgat cctgcacatt
    14341 cagggttccg ataccggtgg cattgccacc ggtaatcgta ttttcagccg taaccagccc
    14401 cccctcgcga atagtcgcct ccccagttcc ttgattacca atttgaaatt caattgagga
    14461 atcattattt tttattagcc actcgccacc cttttcaacg acaacctggc cattactgcc
    14521 cgcctgatag cctaaaatgg cgacgattga actcgtgacg taacccttat ccgtaatatt
    14581 taatgaacct gtgccatagc tacctatttc gaataattcg gtcgtcaaaa ccgagtcctc
    14641 tccctcaaca ttaaccgtcc cgacgcctgc agtttgagta cctaatctta aatagcctcc
    14701 atcgacgtga cccttctgtt taatattcag cgtccccgtt ccggattgac ccacatttaa
    14761 aggccttgca ttatttccgc tatcatacaa tcgccaggtg ccacccaaaa cattaacggt
    14821 accctccgaa tcctcattac cgccaatgac gctggtagta atagtggtta aggcggcatt
    14881 accgccagcc aaaatattaa gcactccagt gccataccag ccaaccaggt aggcatcata
    14941 agcaacatca gtatcaatat tggtaatctg accattatca acttccagac tcgcaccaga
    15001 cactgaaccg cttagtgcca gcaaaacacc cagcgtcaat ctactgaatt tcgttgtcag
    15061 tccagaggat ttacgcaaat taaccgttga tgttttacct accctgcgag ttaattcaga
    15121 gcaggcctga aatacctgta gagtgcaatt ccatatcacg cgatagattc tattcatata
    15181 aacgctccat atacaaacaa tacagccacg aaagagatga gtcgtcagac attaaaaatg
    15241 aaacttatta aattgtcaga ggtctgtatt gagtgttagt tggaggtgcg aaggtgtcaa
    15301 tttggctaat ttgctaatgc tttcgaattg atagattaaa aattgcatag ttcttatttt
    15361 atttaaaaat gaatcatgag ttattgctgc ttaataagaa aactgtttcc ttcaataaga
    15421 aaattatcat catcatattt aacaaagaat agcactaatt gctaaaaacc gaggtttatt
    15481 aaaccccttc ttcatccatt aatgccttta ggaattttcg ttataaaata agggttatgc
    15541 ttaatgcttt aagaaaaata gcaatttccc cttgaatata ttactggtga tggaaggatg
    15601 aatctggaag ctggcattta acaagccagt tctaaaagaa gggaaataag aataactata
    15661 ctcaaaaact aacagccacg gtcatcatga tgtggctgtc aatgaaacta taacccaggc
    15721 gctttccata acgacgtcgt cagtccatga tcaaccagcc ccagctgatc ctgataaact
    15781 gcctgccact tatcgcgtga atcctgataa agttcatgct tttccggggc tggtgtgtgc
    15841 gtccgttccc agcgaaccag ccgttctccg gtttctgcca ttgatgaaaa aattccggca
    15901 ccgacgccag ctgcaatggc acatcctaat gcagtggctt ctttgaccac cggaacattg
    15961 acgggtaatc ccgagacatc agcgagaatt tgactccata atttcccttt tgaacctccg
    16021 cctgcaaaga ctaacgatga aggatgaata ttcgaaaaat cagcaatttg ctgcaagtta
    16081 cacgctgaaa caatcgccgc attttcttcc agcgcacgga acaatgtcgc tttgttacat
    16141 ttatccgggt caatggacaa attaataaag gaaggcgcag cgtgatacca ggttttaaag
    16201 cgcattctgt cggagaagat cggcattacg ccccacgacc caggcggcac ccgactggcc
    16261 atctcttcca gcagcgtata ggtgtcgatg cctaaacgtt cggcaatcaa tttttcttcg
    16321 gcacagaaag catcgcggaa ccagcgcatg gtgagtccgg taaaaaagct tatagattca
    16381 gcttgtacca tgccaggaat tacatgggga ttaacgcgca cgttcatttc tgggtctgtc
    16441 accggcgcgg ctaagtttac aacttgctgc cagaatgtgc cgccaagaac cgcggtttgt
    16501 gccggacgca caacgcctaa ccccaggcaa ccaagctgca cgtcgcctcc tccaacgacc
    16561 acaggagtgc ccgccttcag accgcagagt tccgccgctt gtgaacttac cacgcccagc
    16621 aatgtgccgg tttctttgac aggagaaaga atatcggcac acaggccagc catatccagc
    16681 aatgcaggtt tccagtcacg ggtggttaga tcaagaagtc ccgtggtgcc agcgttagag
    16741 ggatccaccg caagttcgcc gctgagcata taggccagcc agtcgctgat catggtgatg
    16801 gttgatgcct gacggtaaat atcggaacga tggtgcgcca gccaaagtaa tctggggatg
    16861 gcacttaaag ccagtgtttg tccggtcgcg cgataaactt cgttttcaaa ggtattgttg
    16921 tgcagttctt taagttcgct aacttcgcgc gccgctctgg catccacatt ggcgcaggcc
    16981 cagatcggtg ttccttcatt gttatataaa acaatgcctt cacgcatcga acatgccgaa
    17041 acggcagcga tatactccgg ggctatgccg gcgttgtgca acgcctggcg catacactca
    17101 cacgccagtt gccagttttt gttgagatca aattccatag aaccaggaac gtccggtact
    17161 gccagatgcc gccactccgc ctgtcccact gctatttgat tgccttccag gtcgaatatc
    17221 acagcccgaa tacttccggt gcctgcatcc agcgccatca ggtagtactt tgattctgaa
    17281 ggggtaaaga gtcgagccat tagattatcc tcggcttatg cgttctgtct gttcctctat
    17341 acgttctcca tcattgccgg taataaggtc atgcaaattt aactacgtaa aatcgccgct
    17401 gctgtgtcct gatcggtaac cagtgcgttg atataaccgc ctttcattgc agcggcaatt
    17461 gcttcggctt tattttctcc ccctgccacg ccaacccgga cgggtatggt cttcagcgcg
    17521 cttaaaggta agccaatcag ttcgttatgt attttgatat ccgtgacaac gtcacctttt
    17581 gcatcaaaaa agtagcctaa aatgtcgcca accgcccctt ttcggccaat cattaactgt
    17641 tcgccctggc tgatataacc ggagcgaatg atcgtcgcgt cgtcttgttg actcacggcc
    17701 ccaatgccga caatcgccac atccgctgct tgcgcggcta gcagaacatc tttgacgcaa
    17761 ttttcatttt ttagcgtacg ggcaatgtca gcggaggatg cccgcaacgg agccggaata
    17821 atattcacac tgcacgccgc gttaagctgc ccgatacccg tcatataaga accgacgcca
    17881 ccggagagcg tgaccaggcg aatttgctgt gacgaaataa aaccacttaa gcgttgcagc
    17941 gtattcatgg ttgcctcgcc aaaaccaatc gccagcatct gttgtggttg aagtaaactc
    18001 atcaacatat gcgccgcgcc tatccctagt cgcccgccga catcagcatc cgcaagccca
    18061 gggatcaccc ggacatgttg cagcgaaaac tgacgacgta attgagtttc atattccaga
    18121 cagccttcaa agcgagaatt aatctgtacg cgaataatgc cggactgatg ccctttctcc
    18181 agcaatcgcg atactttcaa acgtgtcagg ccgagacgat cgctgatctc actctgggtc
    18241 agcccgtcgt ggtagtaaaa ccacgcgatc cgcgcgacct gttcttcttc acacattccc
    18301 tgttctgaaa ttaccgaatc gttgattgtc ataattcatt cttcactttg aacatattta
    18361 aatctttaat gcaattgttc agttcttgcg catttatatc tgtgatggca accacagttt
    18421 gactctacga gtatgaacaa acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta
    18481 ttcgtcggaa atatgtgcaa tgtccaccta aggttatgaa caaattaaaa gcagaaatac
    18541 atttgttcaa aactcacctg caaaactgaa cgggggaaat atgcaaacga gtgatacccg
    18601 cgcgttaccg ctactttgcg cccgctcggt ttataaacag tattcagggg tcaatgtcct
    18661 gaaaggcatc gattttacgt tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg
    18721 tgccggtaaa tcgacgttaa tgaagattat tgccggtatt acccctgctg atagcggtac
    18781 gctggagatt gggggcaaca actacgccag attaacgcca gttcatgctc atcagctggg
    18841 tatttatctc gttccccagg aaccgctgct tttcccaagc ctgtcgataa aagaaaacat
    18901 cctgtttggg ctggcaaaaa aacagctctc catgcagaaa atgaagaact tgctggcggc
    18961 gctgggctgc cagtttgatc tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca
    19021 aatggtggaa atcctccgcg ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc
    19081 taccgcctcg cttacccctg cggaaaccga acgcttgttt actcgcttgc aagagctgct
    19141 tgctactggc gtgggtattg tttttatctc gcataagctg ccggaaattc gccagattgc
    19201 cgatcgaatt agcgtgatgc gcgacggaac catcgcctta agcggcaaaa ccagcgaact
    19261 gtctaccgac gacattattc aggccatcac gccagtggta cgggaaaaat cgctctctgc
    19321 cagccaaaaa ttatggctgg agttacctgg taaccgccca caacatgccg ccggaacgcc
    19381 ggtgctgaca ctggaaaatc tgactggcga aggcttcagg aatgtcagcc tgacgctcaa
    19441 tgccggagaa attctgggcc tggctgggct ggtgggagcc ggacgcacag aactggccga
    19501 gacgctctat ggtctgcgta ctttgcgtgg cggacgcact atgctgaatg gtaacgagat
    19561 caataaatta tccactggag aacgtttact gcgcggtctg gtttatctgc cggaagatcg
    19621 ccagtcatca ggactgaatc ttgatgcttc gctggcctgg aatgtctgcg cccttactca
    19681 taaccttcgt ggattctggg cgaaaaccgc gaaagataat gccaccctgg aacgatatcg
    19741 tcgggcgctg aatattaaat ttaaccaacc ggaacaagct gcacggacat tatccggtgg
    19801 caaccagcaa aaaatcctca ttgccaaatg cctggaagcc tcgccgcaag tattgattgt
    19861 cgatgagccg acgcgcggcg tggatgtctc ggcgcgtaat gatatctacc aactgttgcg
    19921 cagcatcgcc gcacagaatg tggctgtgct gcttatctcc tccgacctgg aagagatcga
    19981 actgatggca gatcgcgtgt atgtgatgca tcagggcgaa attgcccact ctgcactgac
    20041 cgggcgcgat attaatgtcg agactattat gcgcgttgcc ttcggcgata gtcagcgtca
    20101 ggaggcgtca tgctgaagtt tattcagaac aaccgtgaaa tcacggcact gctggcggtg
    20161 gtgctgctgt ttgtattacc cggttttctc gaccgccagt atttaagtgt gcaaacgctg
    20221 accatggttt atagcagcgc gcaaatcctg atcctgctgg caatgggcgc gacgctggta
    20281 atgcttacgc gcaatattga tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg
    20341 ttggggatgt tactgaacgc aggatattca ctacctgttg cctgtgtcac gactttactg
    20401 cttggtttgc tcgcgggatt tttcaacggt gtcctggtcg cgtggctaaa gattcctgcc
    20461 attgttgcca cccttggcac gttagggttg tacagaggca tcatgttgct gtggactggc
    20521 ggtaaatgga ttgaaaggtt acccgccgaa ctgaaacagc tctccgcacc gctgctgttt
    20581 ggcgtttccg cgattggttg gttgacgata attctggtgg catttatggc ctggctgctg
    20641 gcgaagacgg cgtttggacg cagtttttat gccacgggcg ataatttaca gggcgctcgt
    20701 caactgggcg ttcgtacgga agccattcgc attgtggcat tttcgttgaa cggctgcatg
    20761 gcggcactgg cgggaattgt gtttgcttcg cagattggtt ttatccccaa ccagaccggt
    20821 accgggctgg agatgaaagc aattgcagcc tgcgtgctgg gcggcattag tttgctcggc
    20881 ggttccggtg cgatcatcgg tgcggtactc ggcgcatggt tcctgacgca gatcgatagc
    20941 gtactggtgc tgttgcgcat tccggcatgg tggaacgatt ttatcgcggg cctcgtgctg
    21001 ctggcggtgc tggtgtttga tggacgcctg cgttgtgcgc tggaacgtaa tctacggcgg
    21061 caaaaatatg cccgctttat gacgccaccg ccatccgtta aacccgcttc gtcaggtaaa
    21121 aaacgggagg ccgcataatg cgtattcgct acggttggga actggctctt gccgcactgc
    21181 tcgttattga gattgtcgca tttggtgcaa ttaacccgcg gatgttagat ctcaatatgt
    21241 tgctgttcag caccagtgac tttatctgca ttggcattgt cgccctaccg ctgacgatgg
    21301 tgattgtcag tggcgggatc gatatttcgt ttggttcgac catcggcctc tgcgccattg
    21361 cattgggcgt actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac
    21421 tgctcggcgc attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc
    21481 cgctggtgat tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg
    21541 gtatggccgg agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt
    21601 tcgctaacct ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc
    21661 tcgttttctg gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc
    21721 aaagcccgcg cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct
    21781 atgccatgac ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt
    21841 cagcacgttc cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg
    21901 gcggtgccaa tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat
    21961 tagtgggata tttgcaacaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg
    22021 ccctttccgg tgcgctactt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc
    22081 agcaaattaa agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt
    22141 catgacactt catcgcttta agaaaatcgc cttacttagc gttcttggca ttgccgcaat
    22201 ctctatgaat gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg
    22261 attttttacc agcggtggca acggcgcaca acaagcgggt aaagagctgg gcgttgatgt
    22321 gacctacgac gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt
    22381 cgtcaatcaa ggttataacg ccattatcgt ttctgcggtt tcgcctgatg gcttgtgtcc
    22441 ggcactgaaa cgcgccatgc aacgtggtgt gagagtgctg acctgggact ctgatactaa
    22501 accggagtgc cgctcttact acattaatca gggaacgccc gcccagttgg gaggtatgtt
    22561 ggtggatatg gcggcgcgtc aggtgaataa agacaaagcc aaagtcgcgt ttttctactc
    22621 aagccccacc gttacggacc aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa
    22681 agagcatcct ggctgggaaa ttgtcactac gcagtttggc tataacgatg ccactaaatc
    22741 attacaaacc gcagaaggaa tattaaaagc gtatagcgat ctcgacgcca ttatcgcccc
    22801 cgatgccaac gccctgcccg ctgccgcaca agccgcagaa aacttgaaaa atgacaaagt
    22861 agcgattgtc ggattcagta cgccaaacgt gatgcgtcca tatgtggaac gcggcacggt
    22921 gaaagaattt ggcctgtggg atgtggttca gcaaggcaaa atttcagtgt atgtcgcgga
    22981 tgcattattg aaaaaaggat caatgaaaac gggcgacaag ctggatatcc agggcgtagg
    23041 tcaggttgaa gtctcgccaa atagcgttca gggctatgac tacgaagcgg atggtaatgg
    23101 catcgtactg ttaccggagc gcgtgatatt caacaaagag aatatcggca aatacgattt
    23161 ctgatgtgca ttacttaacc ggagtaagtt atggcagatt tagacgatat taaagatggt
    23221 aaagattttc gtaccgatca accgcaacaa aatatccctt ttaccctgaa aggttgcggt
    23281 gcgctggatt ggggaatgca gtcacgctta tcgcggatat ttaatccgaa aacgggtaat
    23341 accgtgatgc tggcctttga tcatggttat tttcaggggc cgactaccgg acttgaacgc
    23401 attgatataa atatcgcccc gctgtttgaa catgccgatg tattaatgtg tacgcgcggc
    23461 attttgcgca gcgtagttcc cccagcgacc aataagccgg tggtactgag ggcgtcaggt
    23521 gcgaactcta ttctggcgga attaagtaat gaagccgtgg cgttatcgat ggatgatgcc
    23581 gtgcgcctga acagttgcgc ggtggcggcg caggtttata tcggcagcga atatgaacat
    23641 cagtcgatca aaaatattat tcagctggtt gatgccggaa tgaaagtggg aatgccgacc
    23701 atggccgtga ctggcgtggg caaagatatg gtgcgcgatc agcgttattt ctcgctcgcg
    23761 actcgaatcg ccgctgaaat gggggcgcaa attatcaaaa cctattatgt cgaaaaaggt
    23821 tttgaacgga ttgttgccgg atgtccggta cccattgtta ttgctggcgg taaaaaatta
    23881 ccggagcgcg aggcgctgga aatgtgctgg caggctatcg atcagggcgc ttctggtgtg
    23941 gatatggggc gtaatatttt ccagtctgac catccggtgg cgatgatgaa agccgtacag
    24001 gcggtggttc accataacga aacggctgat cgagcatatg aactctatct gagtgaaaaa
    24061 cagtaactgc ggatctaagg agaagaatta tgcacgtcac actggttgaa attaacgttc
    24121 atgaagacaa ggttgacgag tttatcgaag tttttcgcca gaaccacctg ggctctgtac
    24181 aggaagaagg caatttgcgc ttcgatgtct tacaggaccc ggaagtgaat tcgcgctttt
    24241 atatctacga agcctataaa gatgaagacg cagtggcgtt ccataaaacc acgccccact
    24301 acaaaacctg tgtcgcgaaa ctggaatctt taatgaccgg gccgcgtaaa aaacgtctgt
    24361 tcaatggttt gatgccgtga ggcgaattta tcaattttat ctacaattgg ggtaacgcgc
    24421 tgacgggagt aaaaaaatgt ctgactggaa cccctcttta tatttgcact tttccgctga
    24481 acgatcgcgt ccggcggtgg agctgcttgc cagagtgccg ctggaaaatg tcgaatatgt
    24541 tgccgatctt ggctgtggcc caggtaacag caccgccctt ctacaacaac gttggcctgc
    24601 ggccaggata acaggcatcg actcgtctcc ggcgatgatt gctgaagcgc gcagtgcttt
    24661 gccagactgc cagttcgtgg aagcagacat ccgcaactgg caaccggtac aggcactcga
    24721 tctgattttt gctaatgcct cactgcaatg gctgcccgac cactacgaat tgtttcctca
    24781 tctggtttct ttacttaatc cgcagggcgt gctggcagta cagatgccag ataactggct
    24841 ggagccgacc catgtgctca tgcgcgaagt tgcctgggaa caaaactacc cggatcgcgg
    24901 gcgtgagccg ttggctggcg ttcatgctta ctacgatatt ttgagcgaag ccggatgtga
    24961 ggtcgatatc tggcgaacca cctactatca ccagatgccg tcacaccagg cgattatcga
    25021 ttgggtgact gccactggat tacgtccgtg gttacaggat ctgaccgaga gcgaacagca
    25081 gctttttctt aagcgctacc atcagatgct ggaagagcag tatccactgc aagagaacgg
    25141 acagatactg ctggcatttc cgcgtctgtt tattgttgct cggcgtatgg agtaaatatc
    25201 acgtcagctg gtaatgacga tcgggaagaa tttttgctgg aatttcggct tcatcgttca
    25261 tctgtaacag gtcaatttca atagcgttgc agatggcatc cagtggtaaa tcattgtttt
    25321 cagtaccgaa cggatcttcc agttcttccg ccagacaatc cagcgaaata aaagtgtagg
    25381 aaatcagcac agagataaaa ggcgtcatgt aatgcaggtc cacgaccagt gcgaacggca
    25441 gcatgataca aaacagataa acggtacgat gcaaaatcag ggtgtaggca aagggaattg
    25501 gcgtgtaggc aatgcgctca cagcctgcca gaaccgctga aatatcatta agccgatcgt
    25561 tgaggctaat aaacaggata tctgaaagct gcccattgcg gcgctgaacc gccaaccact
    25621 cccccattat taacaagata cggttagctg gagagttcga agccagtaca ggctgaagat
    25681 cttcagtctt gagataatga gccagcactt ccgcctgtgg ctgtttgcgt aatgtcatgc
    25741 gtaaacagtg ggcgaaggcg atttgcaacc gggcaaactc ccttacactt gccgaatccg
    25801 gcaatgtcgt ttttacctcg cgcagtaacg accgtgaggc aatcatcaac tgcccccaaa
    25861 gttttcgcgc ttcaacgtaa cgggcgtacc cggcattatt acgaaaacca agaaaaatgg
    25921 cgatggcgac accgagaatg ctgaacggtg cgagggtgaa tttgatgccc agatgcgtgt
    25981 accagggcag catgaaaata acagcgatag aaaagagaaa attgagtagt aagcgcgatg
    26041 atatcttgga taatactgag ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg
    26101 gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc cctattaaac gtgattacga
    26161 tcacattctc aagacgctac ttacaaatta cctacaagct tacaacatta acaggcgctg
    26221 catgtggcat ctgttaatga taaaggcaca taggatgttg taactaatat agtgaaataa
    26281 gaaacccggt cgaaaccggg ttcagaagta acggtgttat tagcacaacg gacgtacagc
    26341 ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta gcaacctgct cgaccagacc
    26401 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaaaacaa tcgctaccag
    26461 ttcctgggtg ccgataacac ggtcacgatg ctggctccag agttgctgat aacgttccag
    26521 ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta cgctcaccgc gatagaaggc
    26581 aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt gtgccttttg ccttctgccc
    26641 tgccagcagc tgcggcagga tgcgagtgcg gaacttggtc ataccgttga gcgcgataga
    26701 cagcagctga tgcttaatgt acgggttacg gaaacgcccg gtgactgcac tggcgaaaga
    26761 ttccagttca tcacgaggca aatccagtac cggaataatt tcttcgtaga tagctttttc
    26821 aacgaatgcg caaatttcag catcgttcat cgcttcacct acggtatcca gccctgcctg
    26881 gaacgccacc ggcaccagcg cggtgtgcgc accgttgagg attgccactt tgcgttcttt
    26941 gtacggttta atatcgtcaa caatcagcac gttgagcgga tatttgtcca gacgcagttc
    27001 agtcgctaag gatttcggtc cctgaatcac aaacaggtaa aagtgttcag cggtgtcgag
    27061 aaaaccatcg tgataaccca gttcttcttc cagtttagcc acttcatcgc gcggataacc
    27121 ggtaacgata cggtccacca gcgtagaaca gaagctgtta gcctgatcca gccattgaat
    27181 aaatgcttct ggcagtgccc actcttgcgc atagcgcagc accagttcac gcaacgcgtc
    27241 gccattgtag tcaatcaact cacacggaat gatgatccaa cctttatcca gcgcaccgtt
    27301 gaaatggcta aagcgttcga acagcagacg ggtcagtttt gccggatagc ttaccgctgg
    27361 cgcgtcatcg aatttatcgc ccgcatggta gctgataccc gcttcggtgg tgttagagaa
    27421 aacaaagcgc atttccgggt tgtgcgccag tttcaggaat tcatcgtatt cactgtagac
    27481 gctgatttca cgattaaccg agcgaatcag acgcgcgtcg ctgaccgctt cccccttctc
    27541 attcaggcca cggataatgg tggtgtacag accatcctgc gtgctcagtg acggcgggaa
    27601 tgaagtttca atcggacgaa caacgaccac gccagaattc agatcggtgt gctcattcag
    27661 gagatcgatt tgccagtcaa caaaggcgcg caggaagtta ccttcaccaa actgaatgat
    27721 acgttctgga tactgtgcac cgggaaaatc gcgacgattt agtgttttca caatgggttc
    27781 ccttctgatt agtcatacaa cctgtttgaa ttggtacgac aggttagcaa actttaatac
    27841 gcagaacccc tgttttgatc aactcctgat gattaatgag cagttttatg agaaaagtgt
    27901 ggcgcggatc atggtttaat cgaggaaaaa cgccttttcc tggatcataa agtggtagaa
    27961 cacattgcat tcaaatcgcg cgtaatgaat aaagatgtca gacaacttcc tcaccgtaac
    28021 gcatagtgct ggtacggttg cgcccatctt tcttcgagcg atacagacac gtatcggctt
    28081 cgaccatcaa tttattaaag tcatcggtca gcgttcggta cgatgcgcga ccactaccga
    28141 cgccaatact taccgtgaga taaagcgttt tttgttgcca ggtgaatggt tgcagttcaa
    28201 cgcctttacg gattttttcc gccattagca gaccatcgac aggattcacc gacggcactg
    28261 caacagcaaa ttcttcgccg cccattcgcg ccaccagccc cttatcaccg acaatcttct
    28321 gaatatgccg ggcaaacacg cttaacactt tatcgccaca ttcatgtcca tagttatcgt
    28381 taatgctttt gaaatagtcg atatcaagca gcatgacagt cagatgttgt gtctgtttca
    28441 gcgatggact tttcagcgcc tcataaagac cggaccgtga gtaaacctga gtcagaaaat
    28501 caaagtcggc tcgcagcgca acttgcttca ttagcgaatt gatcgctgcc acgctaaaag
    28561 aaaccataat tgggcatatc gccatcgtgg caataccgag acgtgcggag aacatttgcg
    28621 gaatggagaa cggcgaaccg accgagatat caatcaccga atttgccacc agtacgattt
    28681 ccaccgcacc ggtgacaaag gtcaacagac atgttacctg cggcgtatag cgcactgcac
    28741 accagattaa tgcaggcagc gggaacgcca gactccccgc tccgccaatg actaccgagg
    28801 caataaccga cacaataagc gcgatggcgg gcatcatctg ttctgcttta aagcgcggca
    28861 gcactccagg aatagccaac gtcagcatac aaggcacgat caacacgccc gttgagaatt
    28921 gctcactgaa ccagtccgca agcaaaggcc agaaatccag actatcaata ctgaccgaac
    28981 caagatagca tgttcgctga cagcggcggc aaataaaggt accctgggaa tgctattttg
    29041 tcgttttccg caattctcat ggctgattca tctgtacaac cagccagtga tagattaccg
    29101 ccacgatata acgttgttgt tcttcgctta acgaaacacc ctgcggaatg ttatgccact
    29161 tagccagaca accgcgacag caggtcgcgg tggcgtgctg agcgataaac accggatgcc
    29221 cgcgcatggg ggtttgctta ccgtcattaa ccggtaatgc cgcagcaaga cgtttagcga
    29281 caaaatccgc cgcatgttgt tcgatgactg gcgcgccttt ctccagacaa tactgacgct
    29341 ctttcatgcc caaacgaaag cgagagcgaa attttgaacg ggataaacgc gcgaagagtg
    29401 gatcgagaga ctgcattaat aaaccgaacg ccctaactgt ttcgttaatt gttcaagaac
    29461 ggcgataccc gcaagcgagt tacctgcatc atccagttcc ggactccaga ctgcgatggc
    29521 catttcatgc ggaacaatcg ccacaatacc gcccccaacg ccagatttcg ccggtagtcc
    29581 cacccgccag gcaaactccc ccgcgttctg gtacatacca ctggtcgcca tcagcgcgtt
    29641 aatttgccgc gcctgcattg gcgtcaccac tggttcatca atatgaatag ctttcccctg
    29701 attagccaga aagacaaacg tccgggccag ctctacacag ctcattttca gagcgcagta
    29761 atgaaagtag ttttgcagaa cggttgtcac gtcatgatgg aaattgccaa acgacttcat
    29821 cagccaggcg atagccgcat ttcgcgcgga atgttcaaat tcggaacgcg ctaccaccgt
    29881 atcgtaggaa atatcagaca caccgcttaa gccgcgcacg acttccagca tacgttgccg
    29941 tggtgcgctt aatcgccctt gcaacatatc gcagaccacc agcgcaccgg cattaatgaa
    30001 cggattacgc ggtataccct gctccatttc cagttgcact aaggaattga acggtgatcc
    30061 agacggatct ttgccgacgc gttgccagat ttcctcttcg gagtaatgac gcatggcgac
    30121 aacgagactc agcactttgg aaatagactg aatggaaaaa cgttcttgcg cgtctccggc
    30181 ctgaaaaagc tgtccgtcaa cggtacagat agcaatcccc aatcgggaac cgtctactgt
    30241 agccagcgcc ggaatataat ccgcgacttt accctgacca atgagcggcc gcacttgccg
    30301 caagatgttt tctaaaattg cattatccat ggcgactgcc actttctact cctggaccgc
    30361 aggtctgaaa agacctgcga gtatatcaga gctgaatatg tcgcgtcaga tccggtcttt
    30421 ccacaccgtc tggatattac agaattcgtg taagccgaaa tgggaaagct cacgaccaaa
    30481 gccactcttt ttcacgccac caaaggccac tcgcgcgtcg ctggcacaat aaccattgat
    30541 aaacacccca ccgcattcca gacgtgccgc catatgtctg gcctgtgttt cgtcagtggt
    30601 aaaaatggtc gctgaaaggc cgaactcact atcattagcc agttccagtg catgttctgc
    30661 atctttcgca atggtgattg ccgcaacggg gccaaacatt tcttcccgaa acgcggtcat
    30721 ttctggggta acattcgcca gaaccgttgg cggatagtag ttacctgccc cagccatctt
    30781 ttccccgccc agtaacaaac gcgcaccctg cgccagggtt ttctccacct gatgatgcag
    30841 ctcatcacgt aaatcaaaac gagccattgg tccgagagcg ttctcttcgt cacggggatc
    30901 gcccattttc aaggctgccg cagctgccac aaaacgttcg gtaaatgccg aagcaattcc
    30961 ctcttcgata ataaagcgtt ttgccgctgc acatacctgt ccggtattct gataacgtcc
    31021 ggctaccgcc gcttttaccg ccagttccag atcggcatcg ttaagcacaa taaacggatc
    31081 cgaaccgccc agttccagta cgcatttttt cagtgccgct ccagcctgtg cgccaatagc
    31141 cgctcccgca cgaacacttc cggtcaccgt gacagcagca atgcgcgagt ctttaatcat
    31201 ctgactgaca ccgtcgttgt cggcattcag ccagccatat acgccttgtg ggatacccgc
    31261 atctttaaac acctgggcaa tgagctgtgc acagcccatc acattcggcg catgtttaag
    31321 taagtagccg ttacctgcaa gaatgatggg aacagcgcca cgcatcacct gccataacgg
    31381 aaaattccac ggcataatcg ccagaatcgt ccccaacggt cgatactcaa taaccgcctg
    31441 ctgattttcc accagcgtag gttccgcctt cagcattgcc ggaccatgtt ctgcatacca
    31501 gtcacacaaa ttcgccgatt tcgccacttc agcgcgcgcc tggttgattg gtttgcccat
    31561 ttcgcgggtg atcatttgcg ccatttcttc gctacgagcg cgcagagcct taccgatatc
    31621 acgcagtttt tcagcacgat aatctatatt tgtctcgcgc cagtcgcgaa agcctgctgc
    31681 cgccagctga agtgcgtttt cgatatcgtc agcgccagcc cacggcagca cagaaagttg
    31741 ttcacccgtg gcaggattta tcgaaattgc atgagttgcc ggagtaatgg tcatcggggt
    31801 atctccttta tgagtcatgg tatgaagata cgcagattta ctcttgcttt aaaatgaata
    31861 atattaagcc acttattcac gaatcgagaa tgctatggat ctgacccaac tggagatgtt
    31921 caacgcggtt gccgaagctg gcagcataac ccaggctgca gcaaaagtgc atcgcgtgcc
    31981 gtcgaatctc actacccgtc tgcgccagct ggaaacagaa ctgggggttg atctgtttat
    32041 tcgcgagaat cagcgtttac gtctctctcc tgccgggcat aactttttac gctatagcca
    32101 acaaattctc gcgttagtgg atgaagcgcg gagcgttgtc gctggcgatg agccgcaagg
    32161 tttattttct cttggttcgc tggaaagcac cgctgcagtg cgcattccag ccacgctggc
    32221 ggaattcaac cgtcgttatc ccaaaattca gttttcactt tccaccggcc cttccggcac
    32281 tatgctggat ggtgtactgg agggaaaact gaatgcggcg tttattgatg gacccattaa
    32341 ccatactgcc atcgacggga taccggtata ccgcgaggaa ctgatgatcg tcacgccaca
    32401 aggatatgcg ccagtaaccc gtgccagtca ggttaatggc agtaacattt atgccttccg
    32461 cgccaattgt tcgtatcgtc gccacttcga gagctggttt catgctgacg gtgccgctcc
    32521 gggaactatc catgagatgg agtcttatca cggaatgttg gcctgtgtga tcgcaggagc
    32581 aggcattgcg cttattccgc gctctatgct ggaaagtatg ccggggcatc accaggttga
    32641 agcgtggccg ttagctgagc aatggcgttg gttaacaacc tggctggtct ggcgtcgtgg
    32701 tgcgaaaacg cgcccgctcg aggcatttat tcaactgctg gaggtgcctg actcggcaag
    32761 acagggatat caatgagcta tttttgatag ttcttgcggc taatatgctc tatatagtga
    32821 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
    32881 tacatatctc ttcctgatga ttttcccgta cgtaatattg cacctcaggt aaaagaagtt
    32941 ttaaaggatt ttattgatgc actcagtacc ataata
//
LOCUS       DACAPB010000031        32696 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.031, whole genome shotgun sequence.
ACCESSION   DACAPB010000031 DACAPB010000000
VERSION     DACAPB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.031"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="IT904_001299"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="IT904_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP3191107.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="IT904_001300"
     CDS             complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="IT904_001300"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAP3191108.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="IT904_001301"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="IT904_001301"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAP3191109.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="IT904_001302"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="IT904_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAP3191110.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="IT904_001303"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="IT904_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP3191111.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            6113..6160
                     /locus_tag="IT904_001304"
     CDS             6113..6160
                     /locus_tag="IT904_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191112.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="IT904_001305"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="IT904_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAP3191113.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="IT904_001306"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="IT904_001306"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAP3191114.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="IT904_001307"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="IT904_001307"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAP3191115.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..10238
                     /locus_tag="IT904_001308"
     CDS             9873..10238
                     /locus_tag="IT904_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAP3191116.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10388..10906
                     /locus_tag="IT904_001309"
     CDS             10388..10906
                     /locus_tag="IT904_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAP3191117.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10896..12122
                     /gene="dgcN"
                     /locus_tag="IT904_001310"
     CDS             10896..12122
                     /gene="dgcN"
                     /locus_tag="IT904_001310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAP3191118.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12138..12620
                     /locus_tag="IT904_001311"
     CDS             12138..12620
                     /locus_tag="IT904_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP3191119.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12696..13043)
                     /gene="rplS"
                     /locus_tag="IT904_001312"
     CDS             complement(12696..13043)
                     /gene="rplS"
                     /locus_tag="IT904_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP3191120.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13085..13852)
                     /gene="trmD"
                     /locus_tag="IT904_001313"
     CDS             complement(13085..13852)
                     /gene="trmD"
                     /locus_tag="IT904_001313"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP3191121.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPDVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13883..14431)
                     /gene="rimM"
                     /locus_tag="IT904_001314"
     CDS             complement(13883..14431)
                     /gene="rimM"
                     /locus_tag="IT904_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP3191122.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14450..14698)
                     /gene="rpsP"
                     /locus_tag="IT904_001315"
     CDS             complement(14450..14698)
                     /gene="rpsP"
                     /locus_tag="IT904_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP3191123.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14835..16196)
                     /gene="ffh"
                     /locus_tag="IT904_001316"
     CDS             complement(14835..16196)
                     /gene="ffh"
                     /locus_tag="IT904_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP3191124.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16288..17154
                     /locus_tag="IT904_001317"
     CDS             16288..17154
                     /locus_tag="IT904_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAP3191125.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPEMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            17175..18461
                     /locus_tag="IT904_001318"
     CDS             17175..18461
                     /locus_tag="IT904_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP3191126.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18516..19109)
                     /gene="grpE"
                     /locus_tag="IT904_001319"
     CDS             complement(18516..19109)
                     /gene="grpE"
                     /locus_tag="IT904_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP3191127.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19232..20110
                     /gene="nadK"
                     /locus_tag="IT904_001320"
     CDS             19232..20110
                     /gene="nadK"
                     /locus_tag="IT904_001320"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAP3191128.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20196..21857
                     /gene="recN"
                     /locus_tag="IT904_001321"
     CDS             20196..21857
                     /gene="recN"
                     /locus_tag="IT904_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP3191129.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22006..22347
                     /gene="bamE"
                     /locus_tag="IT904_001322"
     CDS             22006..22347
                     /gene="bamE"
                     /locus_tag="IT904_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAP3191130.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22409..22699)
                     /locus_tag="IT904_001323"
     CDS             complement(22409..22699)
                     /locus_tag="IT904_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAP3191131.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22689..23165)
                     /gene="ratA"
                     /locus_tag="IT904_001324"
     CDS             complement(22689..23165)
                     /gene="ratA"
                     /locus_tag="IT904_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAP3191132.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23297..23779
                     /gene="smpB"
                     /locus_tag="IT904_001325"
     CDS             23297..23779
                     /gene="smpB"
                     /locus_tag="IT904_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP3191133.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            23994..24356
                     /gene="ssrA"
                     /locus_tag="IT904_001326"
     tmRNA           23994..24356
                     /gene="ssrA"
                     /locus_tag="IT904_001326"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24530..25243
                     /locus_tag="IT904_001327"
     CDS             24530..25243
                     /locus_tag="IT904_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191134.1"
                     /translation="MDFNLNKKMPVNRYLVIIVFVLFILACMQFIYLCLRSLSIAPNY
                     TGTVSDIIIATTNIILATYAVRAFKNLFKDKLSDNAISKIDTALMSLDDCIDSFSSLF
                     LNMTLIKIYKNTNNPKDPQLVKQLDEAAKNIEKATSSAYKARSIIDSLTRWNISLNTE
                     KGIRLNQLTADVFELGMQATNMYIALINEINPNSPYNSLTHRPFDELFNEFNEERIRV
                     EDESNELKEFSIHEIFKIK"
     gene            complement(25346..25561)
                     /locus_tag="IT904_001328"
     CDS             complement(25346..25561)
                     /locus_tag="IT904_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein B"
                     /protein_id="HAP3191135.1"
                     /translation="MMNCPLCGQAAHTRSSFQVSSQTKERYNQCTNIECGHTFVTHET
                     FVRSVCRPEKISAAPPHPKGMQEQLVY"
     gene            complement(25630..26730)
                     /locus_tag="IT904_001329"
     CDS             complement(25630..26730)
                     /locus_tag="IT904_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP3191136.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSATGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIGDLEME"
     gene            complement(26727..27212)
                     /locus_tag="IT904_001330"
     CDS             complement(26727..27212)
                     /locus_tag="IT904_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3191137.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSTQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            complement(27209..30286)
                     /locus_tag="IT904_001331"
     CDS             complement(27209..30286)
                     /locus_tag="IT904_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP3191138.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRAAQASSKELAGAIRNSRDALK
                     QLNQVGNSLEKFRKLQADNKKLGDRLNYARQKANLLSSELEAMEQPSQRHLVALGRQT
                     LAVQRLEEQQKYLQKQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSITGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTALLTGNVLKTVAGAPIALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGTVLRVLASGPLAMLRVALYAVSGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSEAFEPLKPVFQWIGDKVQVLWGWFTDLLTPV
                     KSTSVELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEVAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVTGMMGVAAAPAELPPLHPLALPAKGGEAIVSRAATVPPV
                     YRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            complement(30279..30398)
                     /locus_tag="IT904_001332"
     CDS             complement(30279..30398)
                     /locus_tag="IT904_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP3191139.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRCGANDE"
     gene            complement(30413..30715)
                     /locus_tag="IT904_001333"
     CDS             complement(30413..30715)
                     /locus_tag="IT904_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP3191140.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            complement(30770..31285)
                     /locus_tag="IT904_001334"
     CDS             complement(30770..31285)
                     /locus_tag="IT904_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP3191141.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKSTVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            complement(31295..32467)
                     /locus_tag="IT904_001335"
     CDS             complement(31295..32467)
                     /locus_tag="IT904_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAP3191142.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKTGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDNKAVATELLGVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNADATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFVFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRNLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            complement(32574..>32696)
                     /locus_tag="IT904_001336"
     CDS             complement(32574..>32696)
                     /locus_tag="IT904_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP3191143.1"
                     /translation="KLTQEETTRLNVVLDYIEAVTATDTSTAPDIIWPVFPASR"
BASE COUNT         8071 a         8845 c         7692 g         8088 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc aaacgagaca ccgagtgttt ctccataaac
      781 tcggacatat ctatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct ctgttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacc
     1981 cggcggcctt tcaacccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag ctgatgttaa caaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gatgaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgcccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgttcaac accaacgctc
     2941 gccagacgct gccgggcaag cagataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgtac tcgctttggc gtctgctgcc ataaacgcct cgcgaacctc cgcccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaactgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc aggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagtag agctacaagc cgcaacaccc tttggcagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gttcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgta gcgcctggcg gtcaaactta cgcagcgtgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgctggttt gcccatcgga tgcaccgcca tatgggtacg tttggtcggg tggcgactga
     3961 ttggctcgtc caccgtgcca cctgcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgcggta
     4141 catcggcaat gggtggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gataataatg tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgttaaccag cactcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcacaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgtcg atcgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccgaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggca tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa cacaaaacgg cagcccttga gctgccgttt ttttattctg tcagttgtga
     5461 aactgaagcg atttagtcgc tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattttca tcacacattt tgacatcagg aacggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga aagggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cgggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tcgcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggctcct
     6601 attcacatct tgccgctcgt cagtacgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaacga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttggtct
     6841 ccggcactac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttgggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg tattgaagca aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac cacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttctatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa ttacccgttc aatgaaggta ttgggctgtt
     7381 acccaagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcgg ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcaaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccagatgcag cccataagca
     7801 aaagtagcaa agtggcgcag agcctgaata aacgccatat tctgatcgtg ctcgacagcg
     7861 ctaatacgat gcagccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggcttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccggg
     7981 ccgaacatcg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccgataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gatagtgtca gcatcttctc gaacaggcgt cccatctgac cgccaccgcc gacgataacc
     8281 accggacgca gtgacggaca aagtgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcgc gccgcgaggc caacatagat gcctcgcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatcac atggccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaaac caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 aggaccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggctcaat ggaaaagcgg ccttcagttg
     9601 ttccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttat ttacacaggc
     9781 aatttagtcg cgatttcaac ccttacttct gtatagataa atttacactc cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaatgccagc acaatacgat taaattaatt aaaaccccac aaataaatta
    10261 aacataagat tttactcatt gtgttttatt tctcacattg atgacgattg catgaaaaaa
    10321 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
    10381 tccctgaatg cgtttttctc accgactgtt cttacttctg attctcctgc tgacgggagc
    10441 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
    10501 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
    10561 atcttcacgt tttagtaccg ccctacaaga gaatgccgcg acatctttgc cttatctgcc
    10621 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
    10681 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
    10741 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
    10801 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
    10861 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
    10921 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatcaccagc atatttatca
    10981 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg aaacagtacg
    11041 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
    11101 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
    11161 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcgtcctgg cattacaccc
    11221 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cccgccccca
    11281 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
    11341 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
    11401 tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg gtggaagcac
    11461 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
    11521 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
    11581 atgaaatgga agagtggcag cttcgtttac aggctaaaaa tgcgcagctt ctacgtaccg
    11641 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaaca
    11701 cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
    11761 atttcaaata catcaatgat acctggggtc atgcgacggg cgatagagtc ttgattgaaa
    11821 tcgcaaaacg gttagctgaa gttggcgggc tgcgacataa agcataccgc ctgggcggcg
    11881 atgaattcgc tatggtgctc tatgatgtac agtcagaatc tgaagtgcag cagatatgct
    11941 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggtcat cagaccacca
    12001 tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa aaattacaag
    12061 agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
    12121 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
    12181 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
    12241 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
    12301 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
    12361 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
    12421 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
    12481 catgggctat gggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
    12541 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
    12601 tggtgattac taccccttaa aacaccagca aacagataaa aaagggccag ccaattggcc
    12661 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
    12721 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
    12781 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
    12841 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
    12901 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
    12961 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
    13021 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
    13081 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
    13141 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
    13201 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
    13261 acagtaacac tggcggaact tccatccctt ctaacacatc aggccgcgta tagtgcgggc
    13321 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtactc
    13381 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
    13441 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
    13501 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagt tcgctgacgc
    13561 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
    13621 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
    13681 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
    13741 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
    13801 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
    13861 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
    13921 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
    13981 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
    14041 tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
    14101 ggtctttcca gtagtagtcc ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa
    14161 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
    14221 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
    14281 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
    14341 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
    14401 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
    14461 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
    14521 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
    14581 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
    14641 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
    14701 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
    14761 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
    14821 tcgcataagg tcatttagcg accaggaaat cccggcggca tcatcccctt catgcttctc
    14881 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
    14941 tgcttcagaa ggcggttaac gtcctgcacc tgcataccgc aaccggcagc gatacgacgc
    15001 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat cgagttgatg
    15061 atggcttcca tacgcaccag cactttatcg tccatctgcg acttaacatt atccgggatc
    15121 tgccccatgc ccggcagctt gcccatcagg ctagccatgc cgcccatatt tttcatctgg
    15181 cgcagttgct caagaaaatc gttgagatcg aagccgtcgc cttttttcag cttgctggct
    15241 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
    15301 tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
    15361 gttttctcgc caacaccgag aaacttgatc ggtttgccag tgatgtgacg aatagagagc
    15421 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
    15481 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
    15541 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
    15601 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
    15661 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
    15721 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa
    15781 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
    15841 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgcaggc
    15901 ggttgcgccg ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
    15961 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
    16021 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc
    16081 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca
    16141 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
    16201 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
    16261 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
    16321 atactgcttc tctttcttat tgctcaaact gtcgacatca ctatgcccgt ttttgctctg
    16381 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
    16441 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
    16501 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
    16561 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
    16621 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
    16681 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctga gatgctggtg
    16741 cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct gtacgcgctg
    16801 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcatttaa ccaggaaatg
    16861 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
    16921 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acctatttag catggaaaat
    16981 atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
    17041 cattaccatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta
    17101 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
    17161 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
    17221 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
    17281 atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
    17341 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
    17401 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
    17461 ttgcgactgg tgtgctgact tttgtcgtac tggtatttgc tgaggtattg ccgaaaacca
    17521 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
    17581 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
    17641 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
    17701 ctatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
    17761 tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
    17821 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
    17881 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
    17941 aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
    18001 cggacgagat ctattttgtg ccggaaggta cgccgctcag cacgcagttg gtaaagtttc
    18061 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
    18121 tgacggttga agatattctg gaagagattg tcggcgattt cactacgtcg atgtcgccaa
    18181 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
    18241 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
    18301 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
    18361 ttggcgagta cgatatagat attctcgacg tacaggacaa tatgattaag caggtaaaag
    18421 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
    18481 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
    18541 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
    18601 acgttacctg gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg
    18661 tccagcggca cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc
    18721 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
    18781 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
    18841 ttctccagcg cgaatttgtg ggctttttca atatccagtt cagtacgtcg acgcaggttt
    18901 tccatttcgg cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccagc
    18961 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
    19021 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
    19081 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
    19141 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
    19201 catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat
    19261 tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct accgctggct
    19321 gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa
    19381 gaatgtgaaa actggcacgc tcgcggagat tgggcaacta gctgatctcg cggtagtcgt
    19441 tggtggcgac ggtaatatgc tgggcgcggc acgcacactc gcccgttacg atattaaagt
    19501 tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca
    19561 gcaacagtta gccgatgtgc tggaaggcca ctacatcagc gagaaacgtt ttttgctgga
    19621 agcgcaagtc tgtcagcaag attgccagaa acgcatcagc accgcgataa atgaagtggt
    19681 gcttcatcca ggcaaagtgg cgcatatgat tgagttcgaa gtgtatatcg acgagatctt
    19741 tgcgttttct cagcgatctg atggactaat tatttcgacg ccaacaggct ccaccgccta
    19801 ttccctctct gcaggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc
    19861 catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg
    19921 tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact
    19981 gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat
    20041 tcatccgaaa gattacagtt atttcaacac attaagcacc aagctcggct ggtcaaaaaa
    20101 attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat actgtacaca
    20161 ataacagtaa tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca
    20221 actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg
    20281 gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc
    20341 gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt
    20401 ctctgaaaga tacgccagcg gctctgcgct ggctggaaga aaaccagctt gaagacgggc
    20461 atgaatgttt gcttcgtcgc gtgatcagca gcgatggtcg ctcccgtggt ttcatcaacg
    20521 gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg
    20581 gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct
    20641 atgccaatga aacctctcta ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa
    20701 gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac
    20761 tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgaac
    20821 aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga
    20881 atgcattggc attaatggcc gacggtgaag acgcaaacct gcaaagtcag ctttacacgg
    20941 ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata
    21001 tgctggaaga agctaccatc cagattgctg aagccagcga tgaactgcgc cactactgcg
    21061 atcgtctgga tctcgatccc aaccgactat ttgaacttga acagcgcatc tcaaaacaga
    21121 tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat taccagtcgc
    21181 tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg cttgcgctgg
    21241 cggtaacgaa acatcatcag caggcactgg aaatcgcgcg cgcattacac caacaacgcc
    21301 agcaatatgc agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc
    21361 cgcatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc gctgacggtg
    21421 ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca
    21481 aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc aatccaggtc atcacggcgc
    21541 gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa
    21601 cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg
    21661 ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa
    21721 ccgatggtgc gatgacagaa acgcatatgc aatccctgaa taaaaaagcg cggttacaag
    21781 agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag
    21841 aactgcttgc agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg
    21901 taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac
    21961 agatgagcca cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg
    22021 ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac tctggagcga
    22081 gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa
    22141 atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc
    22201 gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt
    22261 gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat
    22321 aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg
    22381 tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc
    22441 tgcgaagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat
    22501 gcacactatc gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat
    22561 cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc
    22621 cctcctgcag cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa gccacctcaa
    22681 cggcaatttt acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata
    22741 ttagccgcca gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac
    22801 tcaaagtcga gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat
    22861 ccaccaatca atttcttgaa cggcccatcc accagattca taagaatact ttggttactg
    22921 gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc
    22981 gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc cggcaaaaac
    23041 tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc
    23101 agtgcggtcc ggctaatctg aggcataaca atttccatca acaaaaatcc aacaaataat
    23161 atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc
    23221 taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt cagattaccg
    23281 atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc
    23341 gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc
    23401 cctgcaaggc tgggaagtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta
    23461 tgtccttctg cgtgacggag aagcgttcct gtttggcgca aacatcacgc caatggcagt
    23521 ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg
    23581 cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc
    23641 cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaagaaaca
    23701 gcacgataaa cgttcagata tcaaagagcg cgaatggcag gtggataaag cgcgtatcat
    23761 gaaaaacgcc caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc
    23821 ctctccggcg gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat
    23881 cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc
    23941 cattgaggct ggtcatggcg ctcataaatc tggtatactt acctttacac attggggctg
    24001 attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg
    24061 taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc
    24121 tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac
    24181 ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta
    24241 gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg
    24301 tagtaccgag gatgtaggaa tttcggacgc gggttcaact cccgccagct ccaccaaata
    24361 aaacaagggg ttacgtgaaa acgtagcccc tttttctttg gtagtggcgg caaaatggcg
    24421 atagctatgg actagggttg ggtacaccca cattgccctc tatccttatg agtgatcgca
    24481 cacacgcctc tactttcaag ctatattcta atctcaatat gaggctacta tggacttcaa
    24541 cctgaacaaa aaaatgccag ttaatagata tcttgtgata atagtttttg tccttttcat
    24601 cttagcctgt atgcagttta tttatctttg ccttcgttca ttaagcattg ccccaaacta
    24661 tacgggaact gtaagtgaca ttataatagc tacaacaaat attattttag ctacctacgc
    24721 cgttcgtgca ttcaaaaact tatttaaaga taaactatcc gataatgcca ttagtaaaat
    24781 agatacagca ttgatgtctc tcgatgattg catagatagt ttttcaagct tatttttaaa
    24841 tatgacactt attaagattt ataaaaacac taataacccc aaggaccctc agttagttaa
    24901 acagctcgat gaagcagcaa aaaacattga aaaagcaaca agttcagcct acaaggcaag
    24961 atctataatt gactcattaa ctcgttggaa tataagctta aacacagaaa aaggaataag
    25021 actaaaccaa cttaccgctg atgtttttga gttaggcatg caggcaacaa atatgtatat
    25081 cgcactaata aatgaaataa accctaactc cccatacaac tctttaactc atagaccatt
    25141 tgatgagtta tttaacgaat ttaatgaaga aaggatcaga gttgaagacg aaagcaatga
    25201 attaaaagag ttctctattc atgaaatatt taaaatcaaa taagttatta tagaataatt
    25261 ttaaaaaatc atttgcattc ttcttgtgtc aaaaatcaaa taactgtctc gatattattc
    25321 aaaacccgcc agcggcgggt tagtatcagt aaactaattg ctcctgcatc cctttgggat
    25381 gcggtggggc ggcactgatt ttctcgggac gacaaacaga acgaacaaag gtttcatgcg
    25441 ttacaaatgt atgcccacac tcaatattag tgcactgatt gtaacgctct ttggtttgag
    25501 aggagacttg aaaactactg cgagtatgtg cggcctgacc acacaacgga caattcatca
    25561 tttatcaact ctcctcatct ttgctatatt tacaataatg atacaccatt attcaatatt
    25621 gagaaccatt tattccatct caagatcacc aatcctcact tcaagctcaa gactggtcgt
    25681 aaaaccgtta tccgggctga cggtatgtgt cagagtcgta atggtccatt ccgcatcatc
    25741 tatcggctgt ttaaagccac tgactttcac tggcatttcc gtgtagagat ctgcccgccc
    25801 ttctgccagt tgtagcgaga atgacgcaac gccgcgttgc aggcgttccc actgcatttt
    25861 cgccgctcgt tcggcgttgc tccggttggc ataagtgcga ttaagtacca gaacgttttc
    25921 atccgtaccc accaggtaat cgccctgctt cgcttctggc tctttcttct gcttcttagt
    25981 tctgcgctta cgcttcaccg tggtgctttc tttcttcgca ggttcgcggg tatgcaacca
    26041 gctggcaatt acgcccgtgt aggctccgcg atctgccagg gtaaagcggt gactgtcgcc
    26101 gtccttgcgt gtgatagtga tcactggcag tggtttacca gtggcgcttt tgccctgccc
    26161 ctgccggatg aatagcagat tgccattttt caccgacgcg atggcaccgt actgtcgcgc
    26221 cagccgcatc agaaaactgc cgtcactctc attagtctgg tctatatgtt ccacgggttt
    26281 atccgacagg tctttaccca gcgccatctt cagcttgtga cgcgcggcta tttccttcac
    26341 cacttcccca actgtggtct tgtgccacga tttttcacgg cgggtattca gggtttcccg
    26401 aaaatcagca cttcgcgccc ggatagtcag gcggtccggt gcgccagtgt gttcaatctc
    26461 gtccaccgtg aatgcccctt tcgggaaaag cggctgcccc ttccagccca gcgccagcgt
    26521 aatgaccgca ccacggcgcg gcagcacgat ttttccgtca gcgtcgtcca gctccagatc
    26581 aagctggtcc gcttcaaagc cccggttatc cgtcagcgta agccccatca ggcggttgtc
    26641 cagcacagtg gttatatccc tgccttcaat actgatgctg aatgccggag ttttgttgcc
    26701 tttgttaagc agttcagagc tgaaattcac gacagcagcc ctcccaccgt tttactgata
    26761 tcgcttaagg cagacgttgc cgtgtcctgc agattattca gttgagtact gagatcaccg
    26821 aacatatcgg acagggattc atccacccgt tttagcgaca gggtgaactc aatcctgcgc
    26881 ggcataccgt cgcggaaaaa ctccgtttta gtctgattca gtccctcaat cacatacatg
    26941 ccgtaaatcg tgccgctacc ttcaatcagg ggccatgctt tcccctgttc tgccatctgc
    27001 tccagtgcca gcaacgacag cctgccgcct gttatctccg gcataagaac accggaaagc
    27061 gtcagcatgt cgttgtccgg tcccagaaac tgcgtggacg gacgacggtt tacccggctg
    27121 tttgccgcat gtcgccagct gcgttgatac tgcagttcct gatacggcac agtgcgaagc
    27181 ataaacacgt acaatcccag caccatcatc atgcgtcgta tcccccctga tcgctgtagt
    27241 tactcctggc ctttgccttc agcctgcgtt cacgttcatc aagctggcgg gccacctccc
    27301 gcgcaatatc ctgcgcactt tgtcctggct gcgtctggat gatgatctgc gtcggtgcct
    27361 caatcctgta aacgggtggc acagtggctg cgcgactcac aattgcttct ccacctttcg
    27421 cgggaagtgc caaagggtgc aacggtggaa gctctgctgg cgcggcagca actcccatca
    27481 ttccggtaac aacggcagcc agtgcagctg tatttctccg gctggtcaca tttgccgggc
    27541 cgttaacaat ttccggcccg ttttcaccga cgatgccaaa ctgcccgcgc gggatatagc
    27601 cgccgctgtc atacatcccc gcaaagccat atccccatga cggaaaacca cccgatggca
    27661 tcatcacttt accgtcagca ttcaccgtcg caggttgctg gcgcgtcacg ctttccggca
    27721 gttttgcctt tgcgacctct ttactgacaa tgccgagctt ctccagcaac caggaaacgc
    27781 cggatttcag ggagtccagc ggatgcatga ccatattcag cccttccgcc agtgcctccc
    27841 cgaatcgtcg ccccattgcc gctgcactct gcagttcgac agaggtcgac ttaacgggcg
    27901 tcagcagatc agtaaaccag ccccacagca cctgtacttt gtcgccaatc cactggaaca
    27961 cgggcttaag cggttcgaat gcttcactga cgggacctgc cgccgctttg aatccctcca
    28021 ccacgccacc aagaaatgcg gtgatgggtt gccagtattt ccagacaacc agcgccacgc
    28081 ccgccagtgc agtaaccaca agacctatcg gactgagcag agcacctaac agaccagata
    28141 cggcatacag ggcaacgcgc agcatcgcca gtggaccaga tgccagtact cgcagcaccg
    28201 tgcctgcggc ggccagtcca ccgcgcagta ccgccagagg attcataaac atcacagcaa
    28261 cagcacgtaa accggataat ccagaccgca aaagtgcaat cggcgcacct gctacagttt
    28321 tcaggacatt tcccgtcagt aatgccgtgc ggcgcaaaga cgacaacggc gcagtaagta
    28381 aacctgcggc gttgcccgat gaagcaagcc cgcgtcgcag cagtgccagt ggtgcgccag
    28441 ccagccagga caacgcgctg ctggttcggg ttactgctgc cgtaacggaa ggtaacgttt
    28501 tgatacccag cacagagaat cccagacgaa tcactgccag cggccccagc actgcagcca
    28561 gcgccaccgc taaggttccg aggcctacgg taaccgcagc caccacagcg gctactttca
    28621 tcagtgtgcc tgtcagttcc gggttagctt ccacccagcg gcgcaacgcc cccgtgatgc
    28681 ttttcaccgt gtacagaata tccatcagcg gctggcgcag cgtttcgccc aggctgctga
    28741 aggtgttctg cgctccggtt ttgaccagca accactgcgc agaaagtgag tccttgttaa
    28801 tgtcggattc tttctgcatg gagccgagcg catcattgcc cgctgtcagt tttagctggc
    28861 gctgcagttc cggcaggttg tttgccagtt tcgccgcgtc atcgccaaac tctttaccaa
    28921 acaacatggt catggcagac agacgtttat cctgcggcag tgcgttcacc ttctccagta
    28981 cgcgctggat agttcccatc gcatccttcg tcatctgctt ttcaatcact tcaggattga
    29041 gtttcagcag attcatccct tcaaagaaac tcttgctttg catggtggca atggacaatt
    29101 cacgcaccat cgcgtttgct gcactggctg caacctctgg cgcagcgccc agtgtcagaa
    29161 aggtggaacc cagcgccgcc gctttacgat aatccagacg atcagccaca ccgcccaggc
    29221 gttgcatgac atcaatgatg tctgcccctt tcgacatggc gttatcatcc agatagttca
    29281 gcgcatcacc gagctgttca atattgcggg taggaatttt gtagagctgg gcgattttcc
    29341 ccagactttc tgacagttca tccgctggca gctcaaaggc tgttgccgcc tttgctgccg
    29401 tactggcgaa ggccagcagg tcacgtttct ggtcctccca gctgtcgtca gggtttgcga
    29461 cgttcatgcg cgccccacct tcaaccagtg cagcgaaatc caccgcaccg ttttccatcg
    29521 gcaactgttc gctggcagcc ttgatggcat cctgcatttc ataaaaacgt gcagtgcggt
    29581 tgccattatc gtcacgcaga ccattgacct gctttgccac acctttcatg gcatcttcca
    29641 tgctggtata gctttttact gccgccatca ctggtgcgcc cattgccagc cctgcagccg
    29701 tggtggtggc tccggcacct gcaatacgat cgcgcacctc cagcgaacgg gcataactgg
    29761 cacgcgctgc attcatcctg cgctgagctt cccccagtcg cttcagccgc gcctcctgtt
    29821 tcgaaagttc ctggttataa cgtgatgttt cacgggctaa acgggcagtt gctcccgcat
    29881 catctttcgc agaaattccc gcccggtaca gttcagcacg cacaagcgcc gtttgcttct
    29941 gcaaatattt ttgttgttct tccaggcgtt ggactgccag cgtttgccga cctaaagcca
    30001 caagatgcct ttgtgatggt tgctccatcg cctccagctc agagctaagc aaattagcct
    30061 tctgtctggc atagttcagc ctgtcgccta acttcttgtt atcagcctgc agcttgcgaa
    30121 atttttccag gctgttaccc acctgattga gttgctttaa tgcgtcacgg gagtttctga
    30181 ttgcgccagc cagctctttc gaactggcct gtgcagcacg gaatgggcgg gtgagtttgt
    30241 caaccgcatt aagaatgacc tgcaggcgca ggttgttatc actcatcgtt ggccccgcat
    30301 ctctgaatcg ctttataccg ccattccagc acttcggtca gcggcataac gtcagtaacg
    30361 gatggcggcc agtgaaaaat ggtggcgata tctgccacca gatcgtcaac cgtcaggctg
    30421 tcggtaaacc ggcaagcacc gacttcttca acaaaaaagt gacaacctca accgacatgg
    30481 ctgtgagatc tgccgggtcc atctctgcga tttcctgtgc agtcagtgcc ggactggaga
    30541 tgcgggggat cacggtcatc atcgcgttca catccatatc cataatggcc tgcaggcgtg
    30601 tgccgcgcag cgcaccagac tgcggtttac gcaacacaat ttcggtaatt tctgttttac
    30661 cgcgcatgat gggggtatcc agttgaatgg tcttttcagt ctgtttatcg ctcattttgt
    30721 tgtcctgtaa attaggttct ggcgcggcat tccgcgccgt tcagatacat caaaggccga
    30781 gggcgttgcg gtgcgcttcc atcaggtcca caccgtccac aatttccacc atgttgataa
    30841 ggtccacttc atagagcacc tcaccattga tggtcagctt cgcgtagctg ttggtactgg
    30901 tcactttggt ggtgttgctt tcgcccgtct tccactcgcc ggaatccact tctttgtgac
    30961 gtccacgcac gacaagctcc acggcctgca cttccccggt atcgtcacgc tggatagagc
    31021 cggtaaagcg cagctggatg ccatccaccg tggatttgcc catctgctta aacagcagca
    31081 gttcagtacc accaatggaa aattctgtat ccagtgcact gtcatccagt cccagatcca
    31141 catccaccgc ccccggcata ccgccgccgc gatacttctc atatttgcgg gtgaatttcg
    31201 gcagcgtcag cgactcaacg atcccctgcc agttgttccc gtcgttaaac aagttcagat
    31261 gttttaattt acgtggtaaa gccatgttgt ccccttacgc gctgacctgg ctggcgaaat
    31321 tcaccaggta ctgatcggtg atgcgctggc gcagcatcag attttcaagt ggcggcactg
    31381 gcgtgtagtc gtagtcgatg gtgagttttc cggctttaag cgtgtctttg tcgttcaccg
    31441 actcgtccag ccagcaatca ccaccaatga gatagccctg actgaccagg ttgcgcattt
    31501 tggcgcggat accttcgata atgtcgcggg ccagcgacgg gttaagcggt ttatccaccg
    31561 cccacatgtg tgcttctgcc atcgtgtcca tcagcacctg cgccgtgcgg gtgtagtttt
    31621 cgaagacaaa gagcgggtca tcactcaggc agcgggaacc ccagaagcgg aaaccgtctt
    31681 tacgcacaag cgtggtgacg tcgttctggt taagcagtcc cgcatcagtt gccgggtcct
    31741 gcagatccca gaacacatca gcagaaattc cggtgacacc gttcacgccc acgttggaca
    31801 ggcttttgtg ccaccctgtc tgctcatcaa ttttggcacg cagaccaagc gcacgggcgg
    31861 tggcatatgc cgttgcgtcg gcattcagca ccgtgtccca gccagtaaag tcaggccaaa
    31921 tcagcatccc ttcacgctga ctgaagtttt cacggtaagt gatcgcctcc tgcaccgtct
    31981 tgcagccata cgctgacagg taagcaaacc cacgcaggct ttgcgccacg cccagcaact
    32041 cagtagcaac ggctttatta tcgtgccccg gcacgccgag aatgcgcggt ttaacaccga
    32101 gctgtgactg ggcagataac agggctttca taccagtttt tttaccttca gcagtcactg
    32161 cgccgatgat attggtcgtg gtttcttctt ccgtttcacc ctgcggcaca cgcacaacaa
    32221 cggtcacggg ttttgcctgg tcagcgatgg catccagcga gcgggccagc gtaccggatt
    32281 cacccgtctt accgctggca gtaagcacat cagtgattag cacgggttta ttaagaggga
    32341 acatttttgc atcggcatca tcgcccgtgc agaccatgcc cacgatggcg gtgctcaccg
    32401 tggtaatgga tcgagtgcct tcgttgactt caacaacgcg cactccgtgg tggtaatcct
    32461 gagccatagt ggcgaacctc ctgattggat taggcttcgc cctatgttga agtgattgtg
    32521 tctgacaagc agctaagcgc agttgtaccg ttattcacac aaaatgacgg tatttatctg
    32581 cttgcaggga aaacaggcca aataatatca ggtgcggtgc tggtatctgt tgccgtcaca
    32641 gcctcgatat aatccagcac aacgttaagt cgggtagttt cctcttgcgt cagttt
//
LOCUS       DACAPB010000032        32005 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.032, whole genome shotgun sequence.
ACCESSION   DACAPB010000032 DACAPB010000000
VERSION     DACAPB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.032"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(192..683)
                     /gene="yajQ"
                     /locus_tag="IT904_001337"
     CDS             complement(192..683)
                     /gene="yajQ"
                     /locus_tag="IT904_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAP3191144.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            851..1762
                     /gene="panE"
                     /locus_tag="IT904_001338"
     CDS             851..1762
                     /gene="panE"
                     /locus_tag="IT904_001338"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP3191145.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            1725..2315
                     /gene="yajL"
                     /locus_tag="IT904_001339"
     CDS             1725..2315
                     /gene="yajL"
                     /locus_tag="IT904_001339"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAP3191146.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWLDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2369..3817)
                     /gene="thiI"
                     /locus_tag="IT904_001340"
     CDS             complement(2369..3817)
                     /gene="thiI"
                     /locus_tag="IT904_001340"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP3191147.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4023..4265
                     /gene="xseB"
                     /locus_tag="IT904_001341"
     CDS             4023..4265
                     /gene="xseB"
                     /locus_tag="IT904_001341"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP3191148.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4265..5164
                     /gene="ispA"
                     /locus_tag="IT904_001342"
     CDS             4265..5164
                     /gene="ispA"
                     /locus_tag="IT904_001342"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAP3191149.1"
                     /translation="MDFPQQLEACVKQANQALSHFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5189..7051
                     /gene="dxs"
                     /locus_tag="IT904_001343"
     CDS             5189..7051
                     /gene="dxs"
                     /locus_tag="IT904_001343"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP3191150.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7231..8205
                     /gene="yajO"
                     /locus_tag="IT904_001344"
     CDS             7231..8205
                     /gene="yajO"
                     /locus_tag="IT904_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAP3191151.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(8259..8777)
                     /gene="pgpA"
                     /locus_tag="IT904_001345"
     CDS             complement(8259..8777)
                     /gene="pgpA"
                     /locus_tag="IT904_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP3191152.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(8755..9732)
                     /gene="thiL"
                     /locus_tag="IT904_001346"
     CDS             complement(8755..9732)
                     /gene="thiL"
                     /locus_tag="IT904_001346"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAP3191153.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(9810..10229)
                     /gene="nusB"
                     /locus_tag="IT904_001347"
     CDS             complement(9810..10229)
                     /gene="nusB"
                     /locus_tag="IT904_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP3191154.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10249..10719)
                     /gene="ribE"
                     /locus_tag="IT904_001348"
     CDS             complement(10249..10719)
                     /gene="ribE"
                     /locus_tag="IT904_001348"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP3191155.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(10808..11911)
                     /gene="ribD"
                     /locus_tag="IT904_001349"
     CDS             complement(10808..11911)
                     /gene="ribD"
                     /locus_tag="IT904_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAP3191156.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPETVRTLLIPAHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(11915..12364)
                     /gene="nrdR"
                     /locus_tag="IT904_001350"
     CDS             complement(11915..12364)
                     /gene="nrdR"
                     /locus_tag="IT904_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAP3191157.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12515..13054
                     /locus_tag="IT904_001351"
     CDS             12515..13054
                     /locus_tag="IT904_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP3191158.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMH"
     gene            13353..14237
                     /locus_tag="IT904_001352"
     CDS             13353..14237
                     /locus_tag="IT904_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAP3191159.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14414..14761)
                     /locus_tag="IT904_001353"
     CDS             complement(14414..14761)
                     /locus_tag="IT904_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAP3191160.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(14889..15860)
                     /gene="secF"
                     /locus_tag="IT904_001354"
     CDS             complement(14889..15860)
                     /gene="secF"
                     /locus_tag="IT904_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAP3191161.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(15871..17718)
                     /gene="secD"
                     /locus_tag="IT904_001355"
     CDS             complement(15871..17718)
                     /gene="secD"
                     /locus_tag="IT904_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAP3191162.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(17746..18078)
                     /gene="yajC"
                     /locus_tag="IT904_001356"
     CDS             complement(17746..18078)
                     /gene="yajC"
                     /locus_tag="IT904_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP3191163.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18101..19228)
                     /gene="tgt"
                     /locus_tag="IT904_001357"
     CDS             complement(18101..19228)
                     /gene="tgt"
                     /locus_tag="IT904_001357"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP3191164.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19284..20354)
                     /gene="queA"
                     /locus_tag="IT904_001358"
     CDS             complement(19284..20354)
                     /gene="queA"
                     /locus_tag="IT904_001358"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP3191165.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20447..21028
                     /gene="acpH"
                     /locus_tag="IT904_001359"
     CDS             20447..21028
                     /gene="acpH"
                     /locus_tag="IT904_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAP3191166.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(21033..22850)
                     /gene="malZ"
                     /locus_tag="IT904_001360"
     CDS             complement(21033..22850)
                     /gene="malZ"
                     /locus_tag="IT904_001360"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAP3191167.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23006..24379)
                     /gene="proY"
                     /locus_tag="IT904_001361"
     CDS             complement(23006..24379)
                     /gene="proY"
                     /locus_tag="IT904_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAP3191168.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24455..25774)
                     /gene="brnQ"
                     /locus_tag="IT904_001362"
     CDS             complement(24455..25774)
                     /gene="brnQ"
                     /locus_tag="IT904_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAP3191169.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26181..27476)
                     /gene="phoR"
                     /locus_tag="IT904_001363"
     CDS             complement(26181..27476)
                     /gene="phoR"
                     /locus_tag="IT904_001363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAP3191170.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27534..28223)
                     /gene="phoB"
                     /locus_tag="IT904_001364"
     CDS             complement(27534..28223)
                     /gene="phoB"
                     /locus_tag="IT904_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAP3191171.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28413..29615
                     /gene="sbcD"
                     /locus_tag="IT904_001365"
     CDS             28413..29615
                     /gene="sbcD"
                     /locus_tag="IT904_001365"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAP3191172.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            29612..>32005
                     /gene="sbcC"
                     /locus_tag="IT904_001366"
     CDS             29612..>32005
                     /gene="sbcC"
                     /locus_tag="IT904_001366"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAP3191173.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     "
BASE COUNT         8040 a         8720 c         8089 g         7156 t
ORIGIN      
        1 tgtgaaaatc gacagcaaag tgacgaatcg ctttgagata gaacaggcaa ttcgtcaggc
       61 ataaaaaacg cggcgaattg cctgctgcgc tacgcttatc aggcctacaa agtttctgaa
      121 ttacaaacct ttgtaggccg aatcaggcgt tcatgccgca tctgacatga acaaaacgca
      181 catagtcgcg attaatcgcg gaagtttttg aactggaacg gctgaccgag atcgccacca
      241 cgtaccatcg ccatgacagc ctgcaaatca tcacgagatt tgcccgttac gcggatttca
      301 tcgccctgaa tttgcgcctg cactttcagt ttgctgtctt tgatcatctt gacgattttc
      361 ttctgggtcg cgctctcaat gccctgtttc agtttcgctt ccacaaacca ggttttaccg
      421 ctatgaacga tattctccgg tacatccagc gaactgcctt caatgccgcg cttcagcagc
      481 ttggcacgca gaatatccag caactgattg acctggaaat cggactcgct caacactttg
      541 atggttttgc tggcgtcgtt cagctcaaat gaggcttcaa cgttacggaa gtcaaaacgg
      601 gactccactt cgcggctcgc gttatcgacc gcgttacgtg cttcctgaag atcaacttca
      661 gagacaatat cgaaagatgg catcttttcc tctcccttca tttttgatgc gaagcataat
      721 acccgcaaag ttaaataacc gacagtcctg caccaggacg tatcccttaa ttgtgcatag
      781 tcgcaacagc atgcacaatt aagggatagc cttattgaac acccggagtg gttgcgggtg
      841 aggaggaaca atgaaaatta ccgtattggg atgcggtgcc ttagggcaat tatggcttac
      901 agcactttgc aaacagggtc atgaagttca gggctggctg cgcgtaccgc aaccttattg
      961 tagcgtgaat ctggttgaga cagatggttc gatatttaac gaatcgctga ccgccaacga
     1021 tcccgatttt ctcgccacca gcgatctgct cctggtgacg ctgaaagcat ggcaggtttc
     1081 cgatgccgtc aaaagcctcg cgtccacact gcctgtaact acgccaatac tgttaattca
     1141 caacggcatg ggcaccatcg aagagttgca aaacattcag cagccattac tgatgggcac
     1201 caccacccat gcagcccgcc gcgacggcaa tgtcattatt catgtggcaa acggtatcac
     1261 gcatattggc ccggcacggc aacaggacgg ggattacagt tatctggcgg atattttgca
     1321 aaccgtgttg cctgacgttg cctggcataa caatattcgc gccgagctgt ggcgcaagct
     1381 ggcagtcaac tgcgtgatta atccactgac tgccatctgg aattgcccga acggtgaatt
     1441 acgtcatcat ccgcaagaaa ttatgcagat atgcgaagaa gtcgcggcgg tgatcgaacg
     1501 cgaagggcat catacttcag cagaagattt gcgtgattac gtgatgcagg tgattgatgc
     1561 cacagcggaa aatatctcgt cgatgttgca ggatatccgc gcgctgcgcc acactgaaat
     1621 cgactatatc aatggttttc tcttacgccg cgcccgcgcg catgggattg ccgtaccgga
     1681 aaacacccgc ctgtttgaaa tggtaaaaag aaaggagagt gaatatgagc gcatcggcac
     1741 tggtttgcct cgcccctggt agtgaagaga ctgaagccgt caccactatc gatctgctgg
     1801 ttcgcggcgg tatcaaagtc accactgcca gcgtcgccag cgatggtaac ctggcgatta
     1861 cctgctcgcg cggcgtgaag ctgctggcgg atgcgccgct ggtcgaagtg gctgatggcg
     1921 aatatgacgt gatcgtgctg cctggtggca ttaaaggcgc ggagtgtttt cgcgatagca
     1981 ctctgctggt tgaaaccgtt aaacagttcc accgttccgg gcgtatcgtc gcggctattt
     2041 gcgccgcgcc agccaccgtg ctggtgccgc acgatatctt cccgattggt aatatgaccg
     2101 gcttcccgac gctgaaagac aaaattcccg ccgaacaatg gctggacaag cgcgtcgtct
     2161 gggatgcacg ggtaaaattg ctgaccagcc aggggccggg tacagctatc gactttggtc
     2221 tgaaaattat cgacctgttg gttgggcgtg aaaaagccca tgaagtggca tcacaactgg
     2281 tgatggcggc agggatttat aattattacg agtagtgtcg gatgcggcaa acgtcgcatc
     2341 tgaccagatg cgacgtaaaa accacaaatt acgggcgata taccttcaca ttgttaaagc
     2401 cctgctcgcg cagatagagc gcctgcagac ggctcatcac cccgcgctca caccacagca
     2461 gccaagtttt gttctggtcg agatcgccaa atttggtgct cagtttatag aacggcagag
     2521 aaaccacatc aatcccttcg actttcagtg gcttatcttc ctgttcatcg atagaacgga
     2581 tatcgaggat cacgtcgttc gggccgaagc cattgacggt ttccacttcc accacttcct
     2641 gctcggtctg ctgggcgatt tcgcggatat caacgttatt cgcctcctca accactttat
     2701 cgagaatgct gaagtcgaac ttctcttctt ccgcttcaat cttcgattta accgctttca
     2761 ccgtcgggct tttggagatc acgccacaat attccggcat cgtgcgagca aagtcttcag
     2821 tgccaatctg gcgggccagg ttgatgatgt gctctttgtc gtaagaaatc agcggacgca
     2881 ggatcagcgt atcagagacg ttatcaatca ggcgcaggtt ggtcagcgtc tgactggaca
     2941 cctggccgag cgcttcgccg gtgaccagcg cctgtacgcc gtaacgttca gccactttag
     3001 acgcggcacg caccatcata cgtttgagga taacgcccat ctgaccgtcg tcgattttct
     3061 cgagaatttc cccgacgacc ggttcgaaat taatagcgac aaaacgcacg cggtgggagc
     3121 tgccaaaacg attccacaga taatgcgcca cctgacgcac gccaatttca tgcgccgcgc
     3181 cgccgaggtt aaagaagcag taatgcacgc ggcagccgcg acgcatcaac atataactgg
     3241 aaacaccgga gtcgaaacca ccggaaatga gcgacagcac atcttcctgg gtgccgatcg
     3301 ggaaaccgcc aataccttcg tagcggcctt taatcagcag gagacgatcg tcttccactt
     3361 ccagatggac agtcacttcc ggattggtca gcttcacgcg cgcggactca atatgctgat
     3421 ttaaaccgcc gccgacgtaa cgttccacat caatcgagct aaaatcatgt ttgccacggc
     3481 gcttcacgcg tacgcagaag gttttgcctt ccagctgatc gcgatactga accaacgctt
     3541 tctcgaaaat atcgtgcatg tcggtaaacg gcacgtcttc gacttcgaga atatggtgga
     3601 tacccggaat acgggtcagg gcgtcgcgaa tagccagacg ctggttttca tcttttgcgc
     3661 gaacttcgat gttatcccag tggcggacga cagcgagcgt ctcatcatag tgctttaaaa
     3721 cgttacgaat gttcccggta aggattttta taaagcgcaa gcgcacagat tggcttttga
     3781 tggtgatttc cgggaacaat ttaatgataa acttcatggc ggcaatggtt cgttggcaag
     3841 ccttaagcga cttgtatagg gaaaaataca gcagcccaca cctgcggctg catccaggcg
     3901 cggaagtata ccactaacat cgctttgctg cgcacatcac cttaccattg cgcgttattt
     3961 gctatttgtc ctgagtccgt taccatgacg gggcgaaaaa tattgagagt cagacattca
     4021 ttatgccgaa gaaaaatgag gctcccgcca gctttgaaaa ggcgctgagc gagctggaac
     4081 agattgtaac ccgtctggaa agtggcgacc tgccgctgga agaggcgctg aacgagttcg
     4141 aacgcggcgt gcagctggca cgtcaggggc aggccaaatt acaacaagct gaacagcgcg
     4201 tacaaattct gctgtctgac aatgaagacg cctctctaac cccttttaca ccggacaatg
     4261 agtaatggac tttccgcagc aactcgaagc ctgcgttaag caggccaacc aggcgctgag
     4321 ccattttatc gccccactgc cctttcagaa cactcccgtg gtcgaaacca tgcagtatgg
     4381 cgcattatta ggtggtaagc gcctgcgacc ttttctggtt tatgccaccg gtcatatgtt
     4441 cggcgttagc acaaacacgc tggacgcacc cgctgctgcc gtagagtgta tccacgctta
     4501 ctcattaatt catgatgatt taccggcgat ggatgatgac gatctgcgtc gcggtttgcc
     4561 gacctgccat gtgaagtttg gcgaagcgaa cgcgattctc gctggcgacg ctttacaaac
     4621 gctggcgttc tcgattttaa gcgatgctga tatgccggaa gtgtcggatc gcgacagaat
     4681 ttcgatgatt tctgaactgg cgagcgccag cggtattgcc ggaatgtgtg gtggtcaggc
     4741 gttggattta gacgcggaag gcaaacacgt acctctggac gcgcttgagc gtattcatcg
     4801 tcataaaacc ggcgcattga ttcgcgccgc cgttcgcctt ggcgcattaa gcgccggaga
     4861 taaagggcgt cgtgctctgc cagtactcga caagtacgca gagagcatcg gccttgcctt
     4921 ccaggttcag gatgacatcc tggatgtggt aggagatact gcaacgttgg gaaaacgcca
     4981 gggtgccgac cagcaacttg gtaaaagtac ctaccctgca cttctgggtc ttgagcaagc
     5041 ccggaagaaa gcccgggatc tgatcgacga tgcccgtcag tcgctgaaac aactggctga
     5101 acaatcactc gatacctcgg cactggaagc gctagcggac tacatcatcc agcgtaataa
     5161 ataaacaata agtattaata ggcccctgat gagttttgat attgccaaat acccgaccct
     5221 ggcactggtc gactccaccc aggagttacg actgttgccg aaagagagtt taccgaaact
     5281 ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc cgttccagcg ggcacttcgc
     5341 ctccgggctg ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca acaccccgtt
     5401 tgaccaattg atttgggatg tggggcatca ggcttatccg cataaaattt tgaccggacg
     5461 ccgcgacaaa atcggcacca tccgtcagaa aggcggcctg cacccgttcc cgtggcgcgg
     5521 cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca acctccatca gtgccggaat
     5581 tggtattgcg gttgctgccg agaaagaagg caaaaatcgc cgtaccgtct gtgtaatcgg
     5641 cgatggcgcg attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg
     5701 tcctgatatg ctggtggtcc tcaacgacaa cgaaatgtcg atttccgaaa acgtcggcgc
     5761 gctcaacaac catctggcgc agctgctttc cggtaagctt tactcttcac tgcgcgaagg
     5821 cgggaaaaaa gttttctctg gcgtgccacc cattaaagag ctactcaaac gtaccgaaga
     5881 acatattaaa ggcatggtag tccctggcac gttgtttgaa gagctgggct ttaactacat
     5941 cggcccggtt gacggtcacg atgtgctggg gcttatcacc acgctgaaga acatgcgtga
     6001 cctgaaaggc ccgcagttcc tgcatatcat gactaaaaaa ggtcgtggtt atgaaccggc
     6061 agaaaaagac ccaatcactt tccacgccgt gcctaaattt gatccctcca gcggttgttt
     6121 gccgaaaagt agcggcggtt tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga
     6181 aacggcagcg aaagacaaca agctgatggc gattactccg gcgatgcgtg aaggttccgg
     6241 tatggtcgag ttttctcgta aattcccgga tcgctatttt gatgtagcga ttgccgagca
     6301 acacgcagtg acctttgctg cgggactggc cattggtgga tacaaaccca ttgtcgcgat
     6361 ctactccacc ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg caattcaaaa
     6421 actcccggtc ctgttcgcca tcgaccgcgc gggcattgtt ggtgctgacg gtcaaaccca
     6481 tcagggtgct tttgatctct cttacctgcg ctgcataccg gaaatggtca ttatgacccc
     6541 gagcgatgaa aacgaatgtc gccagatgct ctataccggc tatcactata acgatggccc
     6601 gtcagcggtg cgctacccgc gtggcaacgc ggtaggcgtg gagctgacgc cgctggaaaa
     6661 actgccaatt ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga tccttaactt
     6721 tggtacgctg atgccagacg cggcgaaagt cgctgaatcg ctgaacgcca cgctggtcga
     6781 tatgcgtttt gtgaaaccgc ttgatgaagc gttaattctg gaaatggccg ccagccatga
     6841 agcgctggtc accgtagaag aaaacgccat tatgggcggc gcaggtagcg gagtgaacga
     6901 agtgctgatg gcccatcgta aaccagtacc cgtgctgaac attggtctgc cggacttctt
     6961 tattccgcaa ggaactcagg aagaaatgcg cgccgaactc ggcctcgatg ccgccggtat
     7021 ggaagccaaa atcaaggcct ggctggcata atccctactc cactcctgct atgcttaaca
     7081 aattattcat agactctaaa taattcgagt tgcaggaagg cggcaaacga gtgaagcccc
     7141 aggagcttac ataagtaagt gactggggtg aacgaatgca gccgcagcac atgcaacttg
     7201 aagtatgacg agtatggcag gagtggcagc atgcaataca accccttagg aaaaaccgac
     7261 cttcgcgttt cccgactttg cctcggctgt atgacctttg gcgagccaga tcgtggtaat
     7321 cacgcatgga cactgccgga agaaagcagc cgtcccataa ttaaacgcgc gctggaaggc
     7381 ggcataaatt tctttgacac cgccaatagc tattccgatg gcagcagcga agagatcgtc
     7441 ggtcgcgcac tgcgggattt cgcccgtcgt gaagacgtgg tcgttgccac caaagtattc
     7501 catcgcgttg gtgatttgcc ggaaggatta tcccgtgcgc aaatattgcg ctctatcgac
     7561 gacagcctgc gccgtctcgg catggattat gtcgatatcc tgcaaattca tcgctgggat
     7621 tacaacacgc cgatcgaaga gacgctggaa gccctgaacg acgtggtaaa agccgggaaa
     7681 gcgcgttata tcggcgcgtc atcaatgcac gcttcgcagt ttgctcaggc actggaactc
     7741 caaaaacagc atggctgggc gcagtttgtc agtatgcagg atcactacaa tctgatttat
     7801 cgcgaagaag agcgcgagat gctgccactg tgttatcagg agggcgtggc ggtgattccg
     7861 tggagcccgc tggcgcgggg gcgactgacg cgtccgtggg gagaaactac cgcacgactg
     7921 gtgtctgatg aggtggggaa aaatctctat aaagaaagcg atgaaaatga cgcgcagatc
     7981 gcagagcggt taacgggcgt cagtgaagaa ctcggtgcaa cacgagcaca agttgcgctg
     8041 gcctggttgt tgagtaaacc gggcattgcc gcaccgatta tcggtacatc gcgggaagaa
     8101 cagcttgatg agctattgaa cgcggtggat atcactttga agccggaaca gattgccgaa
     8161 ctggaaacgc cgtataaacc gcatccggta gtaggattta aataagagtc caggcctgat
     8221 gagacgcaac aagcgtcaca tcaggcatcg gtgcacaact acgacagaat gcccagcggc
     8281 cagtgatgac caataaagta caggatgcca gcggagatca ccccggcgac aatatcatct
     8341 atcatgatcc ccatgcctcc atgcacattg cgatcaaacc agcggatcgg ccacggtttc
     8401 cacatatcca gaatacggaa aatcacaaac ccggcggcaa cccactgcca gtcattggtc
     8461 ggcagcgcca tgagcgtgat ccacatacca ataaattcgt cccagacaat gctgccgtga
     8521 tcgtgcacgc ccatatcttt tgcggtctga tgacaaagat agacgccgat acagatcccc
     8581 agcatcacca ccagcgagta gagctgccag ggcaaaaagg tcatcagata ccagaacgga
     8641 atcgctgcca gcgagcccat cgtcccagga acgatcgggc ttaatccact tccgaatccg
     8701 acagcaagca gatgccacgg attactcatc ttcaggcgac ttttcgcgac atctttatgg
     8761 cgtggcaaaa tggtcatatc ctttccagtc aaacgtgaca ggttcgccgt cacgaataaa
     8821 acaaagccct tcgatatcgg cggtcatttg cccgatacag gtaaacggta cgccaaggtg
     8881 tccgagagca acatccagcg cgccacggtt cagttccggc accgtgaaac acaactcgta
     8941 atcttcaccg ccagagagcg cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag
     9001 cgcatcagaa aacggcagca atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac
     9061 gatatgcccg agatcggaaa tcagaccgtc agagagatcg atagctgaat ttgccagatc
     9121 gcgcagtgcc tgcccctgta aaatacgcgg cgatggacgg agatgacgtt tgatcaagta
     9181 gtcagcatct ttagcatcgg caacctgcaa acggttttgc aaaatcgcca gcccggcggc
     9241 gctatcgccc ggtgtaccgg tcacatagat ccagtcaccc ggtttcgccc cagagcgcgt
     9301 taaggctcgt cccatcggaa caaagccgtg gatacccaac gtcattgata atggcccacg
     9361 cgtggtatcg ccgccaatga gttgcatatc gtaataattg agaagatcaa acaaactgtc
     9421 gctgaaggac tcaagccacg cttcgtctac gtccggtaag gttaatgcca gcgtcagcca
     9481 ggccggatcg gcccccatcg ctgccagatc gcttaggttc accgccagtg ctttataagc
     9541 cagatcagca ggatcgatat cagggaggaa atggttaccc gccaccagcg tatcagtgct
     9601 gatcgccagg gtctgtttct cggggatatt tagaagtgcg caatcgtcgc cgatgcccag
     9661 ttcgacatca agacgagaac ttcttacacg gtcaaaataa cgggcaatca gggagaactc
     9721 gccacatgcc atacgttatg cctcagcagg taaaaaagaa aaggccggcg atcatggaac
     9781 ggtcttccgt gaatctaccg gcctggatat cactttttgt tagggcgaat cacaggtgct
     9841 gctttatcga gtacgccgtt gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc
     9901 agttcgatcg cttcgttaat ggccactttg tatggcacat cgctacgttt agacagttcg
     9961 tacagcgcaa tgcgcagtac tgctttttct acctgtccca gttcttccag caggcgggac
    10021 aggtatggct tcatcagtcc gtcgaggtat gcggtattag tcgccacccc ggccagcagc
    10081 tcacggaagt acaggacgtc aacgtctttt acatcctgtt cagccaggaa ctggtattca
    10141 acatcagcga tgtcgttctg ggacaactgc caggagtaga gcgcctggac ggcacactca
    10201 cgagcgcggc gacgagcagc aggtttcacg gatttcccct tactaatttc aggccttgat
    10261 ggctttcaat acattaatca tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc
    10321 agctttggtg ccagcacgtt cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa
    10381 agcaaccgga atttcgctgt cctgggcaac atgcgccagg ccgttgcttg caccaccagc
    10441 gacatattca aagtgggcag tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc
    10501 gtatttaccg gttttagcca gtgcacccgc cgccagcggc agctcatagg caccaggcac
    10561 ccaaacaacg gtaatgtttt catcttttac ctgaccgata cgtttcagtg cgtcaattgc
    10621 accttccagc aggctgtcat tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg
    10681 agcgtccggg gtagcaacgt tagcttcaat aatgttcatt ttcttccttc gggttcgagt
    10741 atggccccgc aggggggcgg attttagcat aatatttcgt gcgctgcttc cctttcgagc
    10801 cgggagatca tgcacctact aaatgcaggc aaacatccgg gcctacatga cgtatctctt
    10861 tgaatttaaa ttggggggcg tcggctaatt tctcaagccc tggcagcgag cataatccac
    10921 gggcgtcgct gcctaatagt ttaggtgcga tatagacaat cagctcatcg actaaacccg
    10981 cctgcagcaa tgcgccagcg agcgttggcc ccgcttccac ccagatgctg ttaatttgct
    11041 gtttacccag ttgcatcatc agtacaacca gatccagatg acctttatgc gctggaatca
    11101 gcaaggtacg caccgtttcc ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg
    11161 tttcgccggg ctgctgcaca atgcgatgta ccggcgtcac gcgattttgg ctatcaatca
    11221 caatacgtat cggctgacgg agattttgtt gcggatagag cgcctgagtt tgttcatcca
    11281 gttcagacca acgcaccgtt aaggcaggat catccgccag caccgtggcg ctgctggtta
    11341 aaatggcatg actttgcgcg cgcagtagtt gtacatcacg ccgcgcctgg ggcgaagtga
    11401 tccactggct ttcgccgctc gccatcgccg tgcgaccatc aagcgatgcg ccaagtttta
    11461 actgaatata aggaaagccg gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg
    11521 cttcactcat catcaggccg tggctgacgt caatgccagc ctgttgcaga cggtaaagtc
    11581 cacgcccagc gacctgcggg ttaggatctt gcatcgaggc aaccacgcgc gctacgccag
    11641 cggcgatgag tgcgtcacag cacggtggcg tacgaccatg atggctacag ggttcgagtg
    11701 tgacataggc ggtcgcacct ttggcttttt cacccgccat acgcaacgcg tgtacttcgg
    11761 catgtggttc acccgcacgt tggtggtaac cttcaccgac aatttcgcca tctttgacaa
    11821 tgacgcaccc gacattcggg ttgggatgcg tggtaaaacg tcctcgttgc gccagcttta
    11881 gcgcccgcgc catgtaatac tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt
    11941 cgccaaattc tttgatatct tcgaaactgc ggtagacaga ggcaaaacgg atataggcga
    12001 ctttatcgag ctttttcaat tgctccatca ccagattgcc aatcatcttg ctcggcactt
    12061 cgcgctcacc ggtggcgcgc agctgcgatt taatatgatt gattgccatt tcgacgtcat
    12121 cggaactcac cggacgtttt tccagcgccc gcagcattcc gctacgcaat ttctcttcat
    12181 taaacggttc acgcacgtcg ttgcttttta caacacgcgg cataaccagc tccgccactt
    12241 caaaggtggt gaaacgttca ttacacacca gacactgccg acggcggcgt acggatgaac
    12301 cctcgcccac gagacgagag tcaattacct tagtgtccac ggcgaaacag aatgggcaat
    12361 gcatacggcg tcctgaccag gtggttaacg gaaatctatt ttaccctgaa ctgacgatac
    12421 aacaaaggcg caacgctttt gagataaagg atcgatgctt tcgctgcttt taccctctac
    12481 cattaggcta attgcgacga gaaagggacg acctatgaat actaacgttt ttcgactgct
    12541 cctgctggga agcctgttca gccttagcgc ctgtgtgcag caaagtgaag tgcgacagat
    12601 gaaacacagc gtcagcacgc tgaaccagga gatgacgcaa ctcaatcaag aaacagtcaa
    12661 aatcactcag caaaacaggc tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg
    12721 agcgaaaaca ccggcaagac tggaaagcca gatcggtact ttacgtatgt cgctggtgaa
    12781 tattacgcct gatacagatg gcaccaccct gacgctacgt attcagggcg agtcgaatga
    12841 tcccttgccc gcattcagcg gaactgttga atatgggcaa attcagggaa caatagacaa
    12901 ctttcaggaa atcaatgtgc agaatcaatt gattaatgcc cctgccagcg tccttgcccc
    12961 cagcgatgtt gatattccgt tacagttaaa ggggatttct gtggatcaat taggctttgt
    13021 acgtatccac gacattcaac ctgttatgca ttaaacgtat tgcggggcaa ttttgtgcgt
    13081 cccgcaacat ctttccccgt cattttgtta ctccgcttac atcacccaga ttgatagtaa
    13141 aagtttgcaa caagggtgaa agtcagtaca atccctgccc gaatgtgtgt aaacgtgaac
    13201 gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg agcaatgatt
    13261 tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc ctttttcact
    13321 cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg gcagccggtg
    13381 cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt
    13441 atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac acccgtttcg
    13501 gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa aaagactggt
    13561 tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc gatgcaaaag
    13621 gtatctggaa ccacggttct ccgctgttta tggaaatcga accacgtttc tccatcgaca
    13681 agctgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc gcgaacaact
    13741 acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac atgggtctgg
    13801 gtaccgatat cgacactggc ctgccgatga gcctgtccat gaacgtctat gcgaaatacc
    13861 agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta
    13921 aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc ggcttcacca
    13981 acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac ggtattaaga
    14041 cccgtactaa taactctatc gcttccagcc atattctggc tctgaactac gatcactggc
    14101 actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac gatgcagaac
    14161 tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt tacctggtag
    14221 taggttacaa cttctgatta tgaaaatgcc gggatttatt cccggcattt ctgattgtta
    14281 acaacgtgtg ctttgttcat gccggatgcg gcgtaaacgc tttgtccggc caacaaaagc
    14341 acgtaaattc aatatattgc tgaaataacg taggcctgat aagcgtagcg catcaggcaa
    14401 ttttacgttt taatcacttc ttcttgttca tcatcgcttt caggtcagcg aatgggttgt
    14461 acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc gtaccatact
    14521 gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacac aacaattccc
    14581 agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga acggtaagtt
    14641 cacgcaggtt ggaataaaca aactcgcggg aacagcgacc gcagacccac ggatagattt
    14701 ttaatgcttt ttcgcgatag ccgctttcta accgcgcata gttttttggg atgatagcca
    14761 tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg cggggatttt
    14821 cgcaatctcc atacatcaaa aaaatcccga tcttctgacc gggattttca acatcaacgg
    14881 gaacttgatt acggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc
    14941 aacatgtgtt cgcgcttcat acccagtttc agagccaacg cagatgccac atagatggaa
    15001 gatgcagtac cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga
    15061 ccaccgaaga ggtacagcat caggataacc atcaaggtag taccggatgt gatcaaggta
    15121 cggtgcagcg tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc
    15181 ttgcggaagt tttcacgaat acggtccgat accacgatac tgtcgttaag cgagtaaccg
    15241 ataaccgaca tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa
    15301 atacccagcg taatgataac gtcgtgcgcc agcgcaataa ccacccctgc cgccagtcgc
    15361 cactcaaagc ggaaacctac gtacacgagg atagacagca gcgctgccat caacgccatc
    15421 gcaccggttt gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact
    15481 gctgcattct gattggtgga ttcgttaatc accttcagaa cctggctgcc caacacctga
    15541 ccgccggttt cgccttcagc aggcggcata cggaccatga tgtcatggct gctaccaaag
    15601 ttttgcagca tcggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt
    15661 tcagccggtt tttcgagcgt aatttcaata accgtaccac cggtgaaatc cagcccccag
    15721 ttaaagccgc gcacgcccat aataacgata gcggcgatta acagcagacc agagatgccg
    15781 aaagcccagt agtcccagcg cataaagtca tagactttac ggccatggtt tagttgttca
    15841 acagtatatt cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc
    15901 gccatatagc aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc
    15961 cacaccgata ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag
    16021 gatgatgact ttaatcagcg tggtgatgtt cgcatcgaag atagaactga atgcgccacg
    16081 ataaccttca tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg
    16141 ttcgttgata agtacgttcg catcgaccgc taccgcaagt gttaagacga tacccgcgat
    16201 acctggcata ctcagcgttg cgcctggcaa cagcgacata atgccgacga tcaagatcaa
    16261 gttggcaatc agagcactgg tcgcaatcag accaaatttc ttatagaaga tgatcatgaa
    16321 cagaatagaa accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat
    16381 acccagggtc gggccaatgg tacgttcttc aacaatctga atcggcgcga tcaacgcacc
    16441 ggcacgcagc agcagtgaca gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg
    16501 gaagctgtta cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac
    16561 cagaaccgca cgaccatttg catctttctt accgctgtct ttgtactcca caaacagggt
    16621 tgccatcggt ttgccgatgt tgtccttagt gaagttagac atgatgttac caccagcgct
    16681 atcgagcgag atgttaacct gcggttggtt gtattcgtcc tggctggaag tggagtcggt
    16741 gatatggtca ccggtcagaa ttacgcgttt gtacagcaca actggctgac cttcgcgggt
    16801 ctgtttcact tcagagtcgc ccggtacgcg accggatgcc gcagcggcct ggtcaacgtt
    16861 ggtgtttacc agacggaatt ccagcgttgc cgtcgcaccc agaatctctt tcgcacgcgc
    16921 agtgtcctga atacctggca gttcaacaac gatacggtca gcaccctgac gctgaaccac
    16981 cggctccgcc acgccaagtt ggtttacacg gttacgcagg atattgatgt tctgctgcac
    17041 cgcatattca cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct
    17101 gccctggcta ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc
    17161 acgagcttta gcatcgcgga aagtaatgct caggccgtag ttgttttctt tacgaacggt
    17221 ggtatacggg atgccctttt cacgcagatc gctgcgcagg ctatcgatat tttgttcctg
    17281 gagtttgcca agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag
    17341 gtcaaggccg agcttcatcg gctcagcgtg aatagctgcc agccagcgcg gcgttgccgg
    17401 ggcaaggtta agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc
    17461 gcgcagctgg gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac
    17521 agacttagca gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc
    17581 actggcggcg actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt
    17641 gggaagcgca tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa
    17701 aggataacgg tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat
    17761 ggtgcctttc ggcaggacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc
    17821 attcagcgcg atagcaatgt agccgttttc cgctactttg gttacgcgac caaccaggcc
    17881 accgttcgtc agaacttcat cacctttggc aatggagtcc atcagctttt tgtgttcttt
    17941 ggtgcgcttc tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag
    18001 catcaaaatc aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc
    18061 agaaataaaa aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg
    18121 gtacttctcg cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt
    18181 caatagcctt gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt
    18241 tgagtcgcgc gcctaatatt tcgttgcaac ggtcaagatg atgcaagtaa gcgcgtgaat
    18301 aattgcgaca ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatact
    18361 tcgcattgcg gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc
    18421 gggttggcat tacgcagtca aacatatcga taccacgacg tacgccttca accaggtctt
    18481 ctggtttacc aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc gggcatacat
    18541 gctccagaat gcggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt
    18601 agccatcaaa accgatatct accagacctt taacagaaat atcacgtaaa tcttcgtaaa
    18661 cgctgccctg gatgatacca aacagcgcat ttttgtttcc gagactgtca aaacgctcac
    18721 ggctacgctt cgcccaacgc agagacatct ccatggagcg ttttgcgtaa tcccagtcag
    18781 caggatacgg cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa
    18841 tctccattga tttttcagga tcgaggaaaa tcggatcgcc gttgatcggg ttacggaagt
    18901 gcacgccctg ttcggtgatt ttacgaatat cgccaaggct gaagacctgg aagccgccgg
    18961 agtcggtgag gatcggcccc ttccactgca taaaatcgtg cagatcgccg tgcagtttca
    19021 tgatttcctg gcccgggcgc agccacaggt ggaaggtgtt gccgaggata atttgcgcgc
    19081 cagtggcttc aacttcttcc ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca
    19141 taaaacaagg cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac
    19201 cgtcggtggt gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa
    19261 cgttttaaac cagcgccgcg gaattactcc ccgacgcgct cattaattgc ctgcggattg
    19321 tacgtgataa acatcgcatc accgtaacta aaaaagcgat atttctcttc taccgctgct
    19381 ttataggcgt tcatggtgtg ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc
    19441 gactctggca agtggaagtt cgtcaccagc gcatcgacca ctttgtactg gaagcccgga
    19501 tagataaaga tttgggtatc gtcgaagaac ggttcaatga gatcgttttt cgctgcctga
    19561 gccgcgcttt ccagcgaacg tactgaagtg gtgccaaccg caatcacccg gttaccgcgc
    19621 gctttcgccg ccagtaccgc gtctaccaca tcctgcggta cttcagcgta ttccgagtgc
    19681 atgatgtgat cttcaatggt gtcgacgcgc accggctgga aggtgcccgc accaacgtgc
    19741 aacgtcacaa acgccatctc cacgcctttg gcgcgcaatt tttccagcaa aggctcgtca
    19801 aaatgcagac ctgcggtcgg ggctgcaacc gcgcccggtt tttcgctata aacggtttga
    19861 taaagttcgc ggtcagcgtc ttcgtccgga cggtcgatat acggcggcag cggcatatgg
    19921 ccgatgctgt tgagaatatc cagcaccgag cgttcatcat taaattcgac ttcaaacagt
    19981 gcgccgtggc gcgcggtcat tgttgcgtta atactttcgt catcgcccag cagcagttct
    20041 gcgccaggtt ttggcgcttt cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc
    20101 atccgttcaa ccagcacttc aatcttgccg ccgctggctt tacgcccaaa caggcgcgcc
    20161 gggatcacgc gggtattatt aaaaaccaga agatcgccgg ggttgagctt atcaagtaaa
    20221 tcggtgaaag taccgtgcgt cagcgcgccc gtcggcccgt ccagcgacag taaacgacag
    20281 ctactgcgtt caggcatggg atagtgggca atcagggatt cgggcaattc aaaggagaaa
    20341 tcggtaacgc gcatgacact gactcagact aaaataagag gcgggtagtc tagtgccggg
    20401 gcgctctccc tgcaaccttt accccctttt ggatagaata taatcgatga attttttagc
    20461 tcacctgcat ttagcccatc tcgcggaaag ctcgctttcc ggcaatttac tggctgattt
    20521 cgtacgcgga aatcctgaag aaagttttcc gcccgacgtc gtggctggca ttcatatgca
    20581 tcgacgtatc gacgtattga ctgacaatct gccggaagtc cgcgaagcac gggagtggtt
    20641 tcgtagtgaa acgcgccgcg ttgcgcctat tacgctggat gtcatgtggg atcactttct
    20701 ttcccgccac tggtcgcagc tgtcgccgga ctttccgcta caggaatttg tctgttatgc
    20761 ccgcgagcaa gtgatgacga ttttgccgga ctcaccgcca cgttttatca atctgaacaa
    20821 ttacttgtgg tcagagcagt ggctggtgcg ctatcgcgat atggatttca tccagaacgt
    20881 gttaaacggc atggcaagcc gccgcccacg tctggatgcc ctgcgtgact cctggtacga
    20941 tttagacgct cattatgacg ccctcgaaac ccgcttctgg cagttttatc cgcggatgat
    21001 ggcgcaggcg tcacgcaagg cgttataaaa cgttagttca tccataccgt agccgaaatg
    21061 gcaggcaaag ccagaatccc gtcagtcagt tgcccatgcc cttctttgca ttgccattgc
    21121 acggcattga gaaacggtga cgcgggtagc accacttcac aggcctcgcc acggttgatt
    21181 gccaccagta cacgttgctg attcagcacg cggacaaata ccaccacgtt atcttccgca
    21241 tacagcacct gacagccgcc atgacgtagc gcctgacttt tcttacgcag cgcaatcatt
    21301 cgctggtaca gcgcgaataa cgccgtatcc tgcttttcca cctgccaggg gaacggttta
    21361 cggcaaaacg gatcgttttt gccatccagt cctacttcat caccgtaata aatgcacggt
    21421 acaccaggcc aggtgaacag ccagaccacc gccagcggca ggcgcgcaat atcccgaccg
    21481 agcagcgttt taaatcgcgc agtatcgtgg ctgtcgagct gattaaacat acgtaattgt
    21541 tgttgatgag aaagccctgc gcggtaatta tccatccagg ccatacaggt ttgggcatca
    21601 atttgctgcg gatcgtaaga gatatcggta ttggcaagaa atccccacaa cgggaatgtg
    21661 aagccacgat agttcatggc ggcatcttcc acatcggcct gtaaccattg ccgtgcatcg
    21721 ccaaaatgtt cgccgacaat atacgcttcc ggctgggttt ctttcgccgc ttcggtgatc
    21781 ccagcaacgt gctgcatatt attgcgcgcc ccacccgcct cccccagcat atgcaccaca
    21841 tccagccgcc agccgtccat actccacggc gctttcagcc aatggcggac aatactgtct
    21901 tccccgcgat aaatttcatt caccagactt tccgactgat aatccagctt cggcaagctg
    21961 gcatagccaa gccagtcgag cgccgtgcca tcatcactaa acgagtacca gtcgcgccag
    22021 ggcgattcgg ggttgtgaca agctcccccc gtgccacgat tgtgcctgtc aaaccaggca
    22081 tgggaatcgc cactgtggtt aaacacgccg tccagcacca gccgcattcc cagctgctgc
    22141 gtattgtgtc gcaaacgcag caacgcccca tcaccgccaa actgcggatc gacatggcga
    22201 taatcctcgg tatcgtattt atgtacgctg ggagctttaa acaccggatt gagatacagc
    22261 gctgtcacgc caagcttttt cagatacggc agtttttcgc ttatcccgtc cagatcgccg
    22321 ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt catcccagtc acgcaagatg
    22381 atctcttgtc cggctgcatg atggtaatag acatgatcct gttcagcttc acgaggaaga
    22441 ctacgcgcaa aacgatcagg gaagatctga taaaaaatct gatccgcagc ccattgtggg
    22501 ccgatatccg gtacatcgac ggcaaactgc tccagtcgtg ccggcggcat tcggctgaag
    22561 ccctgcggtg taaaccaacg ctggcgatcg tgccacagca gtttgaaact gtaacgccgc
    22621 cggggttgtc cgctggagag atcaatcgcc gcacgccatg cggtgacgcc tggctgcggc
    22681 tgactgcgct gcttatgcat tggtactgac atttcttcgt tatcgtgttc tgtacgcagc
    22741 ataatgcgct gcggtgggtc ttcgcccgtc agccacagtg ttatgagcag ttgatctttg
    22801 ctttgtttaa caaatggggg caccggcagg tgccatgcat ttaacatcat aattcccctg
    22861 tgatgaaaat ctgatcacct tgccatagcc taatgcagga tcactcatca tctgaaggat
    22921 atttggggga ggaggacggg ggcggataac tttcggaata gccggatcag gatttcctcc
    22981 tgaaccggct tacagaacgc aagtattatt ggttttcagc cagctgacga tcgtggcgac
    23041 gcttaaacat ccagccaatc aacagcacaa caatccacgc gaaaccgaca tacagcgaga
    23101 tacgcgtatc cgggtgataa ccaatcaacc cgataataaa gagcaggaaa atcagcccgc
    23161 cgatggtcgt tgctacccca cccggcactt taaatttcag cgccttaact tcttctggcg
    23221 gcaaacggcg acggaaggca atttgcgaca gcaggatcat aatccacacc cacaccgtgg
    23281 cgaaggttgc cagcgaagcg atcaccagga agacgttttc cggcatgatg tagttcagat
    23341 acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt aacccacgga ataccgcgac
    23401 gcgacgtttt gctgaaaatt ttcggcgcgc tgccctgctc tgccataccg tggagcatac
    23461 ggcctacgcc aaatacatca ctgttaattg ccgacagcga agcagtcagc acaacaaagt
    23521 taagaatgct ggcggcaaag gtaatgccca tatgctggaa cgtcagcacg aacgggctac
    23581 cggcagtgcc aacctgattc cacgggtaga tagacataat gacgaacagc gtaccgacgt
    23641 agaacaccag aatacgcatc ggcacggagt taatcgcacg cggtatcgat ttctcaggat
    23701 ctttcgcttc accggcggta atcccgataa tttcgatccc accgtaagca aacatcacca
    23761 tttgcaacga cattaccatg ccaagccagc cgttactgaa gaagccgccg ttgctccaca
    23821 ggttatgaat accggtcggt tgcccgccgt tgccaatccc ccagatgatg atgccgaaac
    23881 cggcgacaat catgatgatg atggtggcga ctttaaagaa cgagaaccag aattccagct
    23941 caccgaatac cttcacgctc atcaggttta cggcgcaaat gatcagcacc acgctcagta
    24001 cccaaatcca gtgcggcacc gtcgggaacc agacacccat atagatacca aaagcggtca
    24061 catcggcgat ggcgacaata aggatttcaa agcagtaggt ccagccggta atgtaacctg
    24121 ccagcgggcc gaggttttcc tgcgcataac gcgagaaaga gctggcggcc gggttatgta
    24181 ccgacatttc ccccagcgca cgcataatga tatacgccgc gataccaccg ataatatagg
    24241 ccaacaacac gctcggaccg gccattttga tggcgtctgc cgaaccgtaa aacagcccgg
    24301 tgccaattgc tgaacccagt gccataaagc gtatgtggcg ggtacttagc ccacgcttta
    24361 gcttgttctt actttccatc aattcctaac ccatcaacac aataaaaaac cacggggcat
    24421 cgcagccccg tggttaaaac aaatgttcag tgatttagtg agcgctggag gtcacctgac
    24481 gacctgccgc gcgatcccag ataatggcca gaaccaccat caccactgtt ggcattaacc
    24541 acgccagacc ttgttcggcc agcggtaaac gctgcgccca ggacggtaag atatcgctga
    24601 atgcagatgc tttgatcccg tcgagaatac caaaaagcag gctgataaac atcggcggag
    24661 caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa acttaataca accagtgcga
    24721 tacacggcgg ataaatagcg gtcagcaccg gtacggagat ctggatcagc tggcttaagc
    24781 cgaggttaga aaccaccatc gagaagccgc cgaggataaa caccagcgta cgataagaga
    24841 gcggcacgta ctgggcaaag aattctgcac aagcacaggt caggccaact gccgttacca
    24901 ggcaggcgat gaagattaac gccgccagca ggaagctacc gccgccgcca aaggtgtgct
    24961 gaacgtaagc atgcagaata gcagcgccgt ttgcagactg atcgaccagc gacgcgctgt
    25021 ctgaacccag acggaacagc gccaggtaca gcagagtcag accaacaccc gccatcaggc
    25081 cagcccagac ggtataacgg gtcagtaggc gcgcttcggt aacgccacga gaacgcgccg
    25141 cgttaacaat aacgatacca aacaccattg cgcccagcgt atccatggtc agatagccgt
    25201 taacgaaacc gttagaaaac gcagcgtttt gataagcctc agtcgccgtg ctgatagaac
    25261 ccgccggcca gacaatcgcg gcaacagaca ggatgaccag cgcgataatt ttcagcggcg
    25321 caaggaagtt gcccacggta tccagcagct tgcccggata gagcgaaacc agaataacga
    25381 tagcgaaata gaccaggctg tagataaaca gcggcagcgc ggaatcaccc gtcagcggcg
    25441 caatccccac ttcaaaagaa acggtagctg tacgcggcgt agcgaaaagc ggccccaccg
    25501 ccaggtaaca aaccgttgcc agcagtacgc cagcgacttt accgattggc gtgctgaggc
    25561 tgtcaacacc gccgccaact tttgccagcg ccactactgt taataccggc aggccaacgg
    25621 cagtaatgag gaagccgaat gccgcagtcc agacgtgttc gcctgcctgt aagccgacca
    25681 ttggagggaa aataatgtta cctgcgccga cgaacaacgc aaatgtcata aagcccagag
    25741 cgatgatatc gcgcgatctt aattgatggg tcataaatca tactgcctgt ggatgtggtg
    25801 ttgtaacgtt aaatttttgt gccaatccct ggggatgata cagcggtata tttaaacaat
    25861 tttagcggga aatactccca accaggcgcg gcgaagaata gtttttcgat ttaccgcgtt
    25921 aatgcagtca gtatgacagc acctgaagcg caatttaaac gcttataacg ttttaaagca
    25981 aggagggatc taaaaaccag aggtatatag cgataagaaa tcaccaacca gtataaaaca
    26041 ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa
    26101 atctaacgcc cgtattttga ctgcaaagaa aataagccag catcgactgg cttatggcaa
    26161 taaaagatga caaaggcgga ttaatcgctg tttttggcaa ttaaacgttc cgggataaca
    26221 aaactgaaac gtgttccttt tcctactgta ctctcaatat tcaggcgact ttcgtgatga
    26281 ttcaccgcat gtttcacgat cgctaaccct aatccgctac cgccggtttg ccgggaacgc
    26341 gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc
    26401 ggtccgttat cttcaacgct aaattcggca ccgtgcggca ctcgctgcca gcgtacggtg
    26461 atatgcgtgc cttccggcgt atgattcacg gcgttataga ccaggttcga aatcgcactg
    26521 cgtagctgat cttcattgcc agacaccttg aggccgttat ctatctcaaa ggtaaatgtc
    26581 tgttttttct gactcagagt ctgagcctcg cgctcaacaa cgcgcagcat catcggcaca
    26641 tcaacctttt cattgagcaa atgcgtcggc gcggcttcaa ttttcgacag cgtcagcaat
    26701 tgcttcacca gcccttccat ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttcg
    26761 cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca ggtaaccctg taacacggtc
    26821 aatggcgtgc gtaactcatg gcttacgttg gcgaaaaagt tgcgccgcgc cccttccagt
    26881 tgatgcattt gcgtgacatc acgcgccacc atcagcaact gtttgtgggt ataaggcatg
    26941 acgcgaattt ccagatgccg cccggtgttg agcaccagat tgagcgggcg agaaaaatca
    27001 cgcgttttca gatattgcgt aaactccggg taacgcagta ggttaaggat gttctgcccg
    27061 ttatcttccg gccagcgcaa accaagaatt tgttgcgcca gaccgttaca ccagaaaata
    27121 ccgccctctt ccgtggtcag caccaccgcg tcgggcagcg actccgcgcc gctacgaaag
    27181 cgtttaatca gattgcccag ttcacggcgg cgttttttat ttcgcagctg catctggtgt
    27241 aagccgtata gtagcggttc ccagctacca cgccccggtg gcggggtcat actgcgatcc
    27301 acccacagcc accatgaaag gcgcaataaa ttccagaaat gccagataag cagtcctgtt
    27361 accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg
    27421 aggcagcaaa gtagcagctc cagcaccagc cttttccacg acagccgttc cagcacgtaa
    27481 gatactccag ttaagaaatc ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag
    27541 cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca ccatgcggtc atgcccgccg
    27601 ggctccagtg ctttacgcag gcgacgaatg tggacatcga ccgtgcggtc ttccacatac
    27661 acgttagttc cccagacgtg gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc
    27721 gtcataaaaa agtgcagcag tttaaattct gtcggcccca tctccagcgg ctcttcgccc
    27781 gccatcactc ggtgagatgt cgggtcgaga cttaatccct gcatctcaat cacctcttcc
    27841 accgccattg gcgaaatacg gcgcattacc gctttgattc gcgccaccag ctccttcggc
    27901 gaaaacggct tggtgatata gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct
    27961 tcttcccctc tggcggtcaa catcaccact ggaatatccc gggtcatcga ctcgcgcttg
    28021 aggtgtttga tgaactggat accggagccg ccaggtaaca tccagtcaag gagaattaaa
    28081 tccggccagg gttcattcag ttgattcaca gcactgtcat aatcttccgc ttcgaccggc
    28141 tgaaagccat tttgttcgag cacgaagcag accatttcgc gaattggagc ttcatcttct
    28201 acgaccagaa tacgtctcgc catgatttgc cctgttgtaa taaataggtt gcgatcatta
    28261 atgcgacgtc attatgcgtc agatttatga cagatttatg aaaagctcgt cgcacatatc
    28321 ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa
    28381 tcgcgcccat gctttttcgc cagggaaccg ttatgcgcat ccttcacacc tcagactggc
    28441 atctcggcca gaacttctac agtaaaagcc gcgaagctga acatcaggct tttcttgact
    28501 ggctgctgga gacagcacaa acccatcagg tggatgcgat tattgttgcc ggtgatgttt
    28561 tcgataccgg ctcgccgccc agttacgccc gcacgttata caaccgtttt gttgtcaatt
    28621 tacagcaaac tggctgtcat ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc
    28681 tgaatgaatc gcgcgatatc atggcgttcc tcaatactac cgtggtcgcc agcgccggac
    28741 atgcgccgca aatcttgcct cgtcgcgacg ggacgccagg cgcagtgctg tgccccattc
    28801 cgtttttacg tccgcgtgac attattacca gccaggcggg gcttaacggt attgaaaaac
    28861 agcagcattt actggcagcg attaccgatt attaccaaca acactatgcc gatgcctgca
    28921 aactgcgcgg cgatcagcct ctgcccatca tcgccacggg acatttaacg accgtggggg
    28981 ccagtaaaag tgacgccgtg cgtgacattt atattggcac gctggacgcg tttccggcac
    29041 aaaactttcc accagccgac tacatcgcgc tcgggcatat tcaccgcgca cagattattg
    29101 gcggcatgga acatgttcgc tattgcggct cccccattcc gctgagtttt gatgaatgcg
    29161 gtaagagtaa atatgtccat ctggtgacat tttcaaacgg caaattagag agcgtagaaa
    29221 acctgaacgt accggtaacg caacccatgg cagtgctgaa aggcgatctg gcgtcgatta
    29281 ccgcacagct ggaacagtgg cgcgatgtat cgcaggagcc acctgtctgg ctggatatcg
    29341 aaatcactac tgatgagtat ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat
    29401 cattgcctgt cgaagtattg ctggtacgtc ggagtcgtga acagcgcgag cgtgtgttag
    29461 ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc
    29521 tggcactgga agaactggat gaatcgcagc agcaacgtct gcagcatctt ttcaccacga
    29581 cgttgcatac cctcgccgga gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa
    29641 cctgaactca ttaaaaggcg aatggaagat tgatttcacc cgcgagccgt tcgccagcaa
    29701 cgggctgttt gctattaccg gcccaacagg tgcggggaaa accaccctgc tggacgccat
    29761 ttgtctggcg ctgtatcacg aaactccgcg tctctctaac gtttcacaat cgcaaaatga
    29821 tctcatgacc cgcgataccg ccgaatgtct ggcggaggtg gagtttgaag tgaaaggtga
    29881 agcgtaccgt gcattctgga gccagaatcg ggcgcgtaac caacccgacg gtaatttgca
    29941 ggtgccacgc gtagagctgg cgcgctgcgc cgacggcaaa attctcgccg acaaagtgaa
    30001 agataagctg gaactgacag cgacgttaac cgggctggat tacgggcgct tcacccgttc
    30061 gatgctgctt tcgcaggggc aatttgctgc cttcctgaat gccaaaccca aagaacgcgc
    30121 ggaattgctc gaggagttaa caggcactga aatctacggg caaatctcgg cgatggtttt
    30181 tgagcagcac aaatcggccc gcacagagct ggagaagctg caagcgcagg ccagcggcgt
    30241 cgcgttgctc acgccggaac aagtgcaatc gctgacagcg agtttgcagg tacttactga
    30301 cgaagaaaaa cagttactta ccgcgcagca gcaagaacaa caatcactaa actggttaac
    30361 gcgtctggac gaattgcagc aagaagccag ccgccgtcag caggccttgc aacaggcgtt
    30421 agccgaagaa gaaaaagcgc aacctcaact ggcggcgctc agcctggcac aaccggcacg
    30481 aaatcttcgt ccgcactggg aacgcatcgc agaacacagc gcggcgctgg cgcatactcg
    30541 ccagcagatt gaagaagtaa atactcgctt acagagcaca atggcgcttc gcgcgagcat
    30601 tcgccaccac gcggcgaagc agtcagcaga attacagcag cagcaacaaa gcctgaatac
    30661 ctggttacag gaacacgacc gcttccgtca gtggaacaac gaactggcgg gttggcgtgc
    30721 gcagttctcc caacaaacca gcgatcgcga gcatctgcgg caatggcagc aacagttaac
    30781 ccatgctgag caaaaactta atgcgcttgc ggcgatcacg ttgacgttaa ccgccgatga
    30841 agttgctacc gccctggcgc aacatgctga gcaacgccca ctgcgtcagc acctggtcgc
    30901 gctacatgga cagattgttc cccaacaaaa acgtctggcg cagttacagg tcgctatcca
    30961 gaatgtcacg caagaacaga cgcaacgtaa cgccgcactt aacgaaatgc gccagcgtta
    31021 taaagaaaag acgcagcaac ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat
    31081 caaaacgctg gaagctcaac gtgcacagtt acaggcgggt cagccttgtc cactttgtgg
    31141 ttccaccagc cacccggcgg tcgaggcgta tcaggcgctg gagcctggtg ttaatcagtc
    31201 tcgattactg gcgctggaaa acgaagttaa aaagctcggc gaagaaggtg cggcgctgcg
    31261 cgggcaactg gacgccttaa caaagcagct tcagcgcgat gaaaacgaag cgcaaagcct
    31321 ccgacaagat gagcaagcac ttactcaaca atggcaagcc gtcacggcca gcctcaatat
    31381 caccttgcag ccacaggacg atattcaacc gtggctggat gcacaagatg agcacgaacg
    31441 ccagctgcgg ttactcagcc aacgacatga attacaaggg cagattgccg cgcataatca
    31501 gcaaattatc cagtatcaac agcaaattga acaacgccag cagcagctct taacggcatt
    31561 ggcgggttat gcactgacat tgccacagga agatgaagaa gagagctggt tggcgacacg
    31621 tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg
    31681 tattcagcag ctgacgccga ttctggaaac gttgccgcaa agtgatgatc tcccgcacag
    31741 cgaagaaact gtggcgctgg ataactggcg gcaggttcat gaacaatgtc tcgcattaca
    31801 cagccagcag cagacgttac agcaacagga tgttctggcg gcgcaaagtc tgcaaaaagc
    31861 ccaggcgcag tttgacaccg cgctacaggc cagcgtcttt gacgatcagc aggcgttcct
    31921 tgcggcgcta atggatgaac aaacactaac gcagctggaa cagctcaagc agaatctgga
    31981 aaaccagcgc cgtcaggcgc aaacg
//
LOCUS       DACAPB010000033        31642 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.033, whole genome shotgun sequence.
ACCESSION   DACAPB010000033 DACAPB010000000
VERSION     DACAPB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.033"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..112
                     /locus_tag="IT904_001367"
     ncRNA           <1..112
                     /ncRNA_class="other"
                     /locus_tag="IT904_001367"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            232..573
                     /locus_tag="IT904_001368"
     CDS             232..573
                     /locus_tag="IT904_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAP3191174.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGGDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(564..1490)
                     /locus_tag="IT904_001369"
     CDS             complement(564..1490)
                     /locus_tag="IT904_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191175.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVQRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1580..2578
                     /locus_tag="IT904_001370"
     CDS             1580..2578
                     /locus_tag="IT904_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAP3191176.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2575..2793)
                     /locus_tag="IT904_001371"
     CDS             complement(2575..2793)
                     /locus_tag="IT904_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191177.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2795..4810)
                     /gene="ligA"
                     /locus_tag="IT904_001372"
     CDS             complement(2795..4810)
                     /gene="ligA"
                     /locus_tag="IT904_001372"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP3191178.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4881..5867)
                     /gene="zipA"
                     /locus_tag="IT904_001373"
     CDS             complement(4881..5867)
                     /gene="zipA"
                     /locus_tag="IT904_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAP3191179.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6097..6858
                     /gene="cysZ"
                     /locus_tag="IT904_001374"
     CDS             6097..6858
                     /gene="cysZ"
                     /locus_tag="IT904_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAP3191180.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7043..8014
                     /gene="cysK"
                     /locus_tag="IT904_001375"
     CDS             7043..8014
                     /gene="cysK"
                     /locus_tag="IT904_001375"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP3191181.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8398..8655
                     /gene="ptsH"
                     /locus_tag="IT904_001376"
     CDS             8398..8655
                     /gene="ptsH"
                     /locus_tag="IT904_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAP3191182.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8700..10427
                     /gene="ptsI"
                     /locus_tag="IT904_001377"
     CDS             8700..10427
                     /gene="ptsI"
                     /locus_tag="IT904_001377"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAP3191183.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10468..10977
                     /gene="crr"
                     /locus_tag="IT904_001378"
     CDS             10468..10977
                     /gene="crr"
                     /locus_tag="IT904_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP3191184.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11020..11871)
                     /gene="pdxK"
                     /locus_tag="IT904_001379"
                     /gene_synonym="thiJ"
     CDS             complement(11020..11871)
                     /gene="pdxK"
                     /locus_tag="IT904_001379"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAP3191185.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11976..12350
                     /locus_tag="IT904_001380"
     CDS             11976..12350
                     /locus_tag="IT904_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAP3191186.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCYEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12383..13117
                     /locus_tag="IT904_001381"
     CDS             12383..13117
                     /locus_tag="IT904_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAP3191187.1"
                     /translation="MKKRFIYHDEKSNKFWWIDYEGDSLAVNYGKVGSIGKFQTKEFD
                     NEEQCLKEASKLIAAKMKKGYQEDPKFNFMDRYYFDDEEIGLHVKTSHPNFQCHFTDP
                     LYMCCWDEESPFGSDEGADALNVLENSLRKEPDLDCADFPQMLIETMWGMKYIAMDSI
                     LEEDVRAQLLVDEMSTIQSNMITYATAFGQIKVMGKISHKLKKMGLNALARHQLTAKI
                     LQWGDGQDSPILQKMIDDLTAFPHEN"
     gene            complement(13306..14217)
                     /gene="cysM"
                     /locus_tag="IT904_001382"
     CDS             complement(13306..14217)
                     /gene="cysM"
                     /locus_tag="IT904_001382"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAP3191188.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14351..15448)
                     /gene="cysA"
                     /locus_tag="IT904_001383"
     CDS             complement(14351..15448)
                     /gene="cysA"
                     /locus_tag="IT904_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAP3191189.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15438..16313)
                     /gene="cysW"
                     /locus_tag="IT904_001384"
     CDS             complement(15438..16313)
                     /gene="cysW"
                     /locus_tag="IT904_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAP3191190.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16313..17146)
                     /gene="cysT"
                     /locus_tag="IT904_001385"
     CDS             complement(16313..17146)
                     /gene="cysT"
                     /locus_tag="IT904_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAP3191191.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17146..18162)
                     /locus_tag="IT904_001386"
     CDS             complement(17146..18162)
                     /locus_tag="IT904_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3191192.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(18320..19111)
                     /gene="ucpA"
                     /locus_tag="IT904_001387"
     CDS             complement(18320..19111)
                     /gene="ucpA"
                     /locus_tag="IT904_001387"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAP3191193.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19240..20097)
                     /gene="murR"
                     /locus_tag="IT904_001388"
     CDS             complement(19240..20097)
                     /gene="murR"
                     /locus_tag="IT904_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAP3191194.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            20261..21157
                     /gene="murQ"
                     /locus_tag="IT904_001389"
     CDS             20261..21157
                     /gene="murQ"
                     /locus_tag="IT904_001389"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP3191195.1"
                     /translation="MQFEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21161..22585
                     /gene="murP"
                     /locus_tag="IT904_001390"
     CDS             21161..22585
                     /gene="murP"
                     /locus_tag="IT904_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAP3191196.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            22590..23894
                     /gene="pbp4b"
                     /locus_tag="IT904_001391"
     CDS             22590..23894
                     /gene="pbp4b"
                     /locus_tag="IT904_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HAP3191197.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYYQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(24134..25033)
                     /gene="yfeX"
                     /locus_tag="IT904_001392"
     CDS             complement(24134..25033)
                     /gene="yfeX"
                     /locus_tag="IT904_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAP3191198.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEDNEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(25129..25704)
                     /locus_tag="IT904_001393"
     CDS             complement(25129..25704)
                     /locus_tag="IT904_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAP3191199.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKSANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(25765..26214)
                     /locus_tag="IT904_001394"
     CDS             complement(25765..26214)
                     /locus_tag="IT904_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAP3191200.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(26201..26626)
                     /locus_tag="IT904_001395"
     CDS             complement(26201..26626)
                     /locus_tag="IT904_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAP3191201.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            26840..27709
                     /gene="amiA"
                     /locus_tag="IT904_001396"
     CDS             26840..27709
                     /gene="amiA"
                     /locus_tag="IT904_001396"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAP3191202.1"
                     /translation="MSTFKSLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKASGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            27713..28612
                     /gene="hemF"
                     /locus_tag="IT904_001397"
     CDS             27713..28612
                     /gene="hemF"
                     /locus_tag="IT904_001397"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAP3191203.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPDADPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(28618..29610)
                     /gene="eutR"
                     /locus_tag="IT904_001398"
     CDS             complement(28618..29610)
                     /gene="eutR"
                     /locus_tag="IT904_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAP3191204.1"
                     /translation="MKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHG
                     EFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEIAT
                     RPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPDRVLHMLRNQSALEVKEQHKA
                     ALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTAESISHQSY
                     RRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNA
                     VRRELISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLFSEKPSLTLHQRMREWG"
     gene            29621..29734
                     /locus_tag="IT904_001399"
                     /pseudo
     CDS             29621..29734
                     /locus_tag="IT904_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            30228..30920
                     /locus_tag="IT904_001400"
     CDS             30228..30920
                     /locus_tag="IT904_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP3191205.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQNKAAAIAEFKELQRQQI
                     TAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSKEEQLPEEREQVETEDQ
                     QNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCRYRGNDKYEIWYSRTKM
                     AKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLRIPSKGTAEEASMTAQQ
                     HEREAKVSYLVH"
BASE COUNT         7785 a         8440 c         7891 g         7526 t
ORIGIN      
        1 ctcaaagatt cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc
       61 gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgtaaaa
      121 aagcgcccta aaggcgcttt tttgctatct gcgatttgta agattgattt ttcttctcaa
      181 gttccttgtt cttctaccga tattacctcg tacagtgacg aggactgaac aatggccact
      241 atctcttcaa cttcaattcc ttccatacaa acacagagta gtaacagggc atcacaaggt
      301 ggcgatgttg cttcacaaat cgcgcggata agtcagcaaa tcatcaaact cactcaacaa
      361 attaaagaga ttgtagacac tagcggcagt gctgaagaca agcaaaaaca ggccgaattg
      421 attcaacagc aaatcaccct actggaaaca caactggcgc agttgcagag acagcaggca
      481 gaaaaggcgc aggagaaaga gcagcgtttg tctctgaatg tatcccttct aaatcctgtt
      541 gaaaaaacga ctcatatcga tatctatatc tgatacaacg gatccccttc ccgaccagcc
      601 attagcgcat tgccctggtc tcgtaccacg tcccataacg cttcagcggc ggtggaaagt
      661 gaacggttct tcctgcgcac cagcatcaac tggcgttcaa caaccggcgt aatgcgctgt
      721 accaccagtg ggctaccttc aggcaatggt agcgccagtg cgggaagaat actgatgccg
      781 atgcctgcgg ctaccatcgg gaacagcgtc gccggatgtc caatctcctg cacaatattc
      841 gcctgaatac cattgcgcgc cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa
      901 tcctgcaaca ccaatttcgc tccctgtaat gcctgccagg gtacataatc ttccacggcc
      961 aaagcgctat cgcgatggca aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc
     1021 agatcgccca ctgggcctgg atcgatgacg atgccaaaat cgacatctcc ctggcgaata
     1081 ctttccatta cccactgctg cggacgatcg tgcaggacaa actgaatatc tggatagcgg
     1141 cgatggcttt cagcaatgca ttgcggaata agatgcgcgg aaatggtctg gctggcagcg
     1201 acccgaactt ttccgctcag ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgttc
     1261 agttcatcca gcagtcgctc aagacgcaaa gccagctgct gccctgcatc tgtaagcacc
     1321 acttcacgcg tggttctgtc cagcaggcga acaccagtat gattttccag ttccttcaca
     1381 ctgtgactca ctgccgactg gctcaggccg atacgctctc ctgcacgact aaaacttttc
     1441 tcctgcgcta ctgtgacgaa aacctttaat tgttttaaag aataattcat ctgttttctt
     1501 catgaatgga tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca
     1561 tcgatcatct ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct
     1621 gatcacggtg gtgttgctgg cctctttctt tccggccaga ggcgatttcg tccccttctt
     1681 tgaaaatctg accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc
     1741 gcgtgaggcg attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag
     1801 caccttcgtg ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt
     1861 cgacccgatg ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc
     1921 tgcaatcgcc ttcacgtcaa tggcgggcgg taacgtcgcg gcagcggttt gttctgcgtc
     1981 ggcatccagc ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaatgt
     2041 tcacggtgca gggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc
     2101 gtttgtgttg gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa
     2161 atggattgcg aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga
     2221 agccgtcgtt aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt
     2281 ggtcagctgc gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact
     2341 gagcttcaat aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc
     2401 aaatggcatc ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct
     2461 gcccctgatg attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata
     2521 caaacgccag accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag
     2581 cggacgcttc aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat
     2641 ttgcattagc tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag
     2701 atactcctcc ggcaggtcga ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt
     2761 attggctatt tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt
     2821 ccgcttcgtc gatgacttca atgcccagtt cctgcgcctt cgccagttta gatcctgcag
     2881 cttcacccgc tatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca
     2941 gttcgaccag tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca
     3001 ccacggtttt accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag
     3061 gccagtgaac accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa
     3121 agaagttgtg aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt
     3181 caatcgaagc ggcttccagc gcttccagcg tgccgaaata tgccgccaga cctgctgcgg
     3241 tggcctcgcc gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt
     3301 tcgctttttc cagcgcgtta accacgtttt gtgccgattt tggccccata cgctccagtc
     3361 cggtcagttt gcctgcggtg agtttgaaca gatctgccgg agtatggaca tattcttttt
     3421 caaccagctg atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa
     3481 caaagtgttt cagcgactct ttacgctgcg caccgcaaat caggccaccg gtacagcggg
     3541 caaccgcttc accttccaca cgctcaacgt cagaaccaca taccggacaa tgcgtcggga
     3601 atacaacctc acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga
     3661 tcacgtcgcc agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga
     3721 tttcatccgc attgtgtaag gttgcgttac tcaccagcac gcctgcaaca tggacaggtt
     3781 ccagacgcgc aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca
     3841 caaaggtcat ctgctcctgc gccgggaatt taaacgctac cgcccagcgc ggggcacgcg
     3901 cgacaaagcc aagctgctcc tgctgtgcca gtgagttgac cttaatcacc acgccgtcga
     3961 tatcaaagcc cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt
     4021 ccgccgattc acaaagcgtt acccgatcgc tgaccggcaa cccccactgt ttaaattgca
     4081 gtaaacggcc aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc
     4141 agaaaaaagt gagcggtcgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag
     4201 ctgccgcatt acgtgggtta gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa
     4261 tcttttcgaa ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga
     4321 tattctctcc gtgtagcttc agtggaatgg cgcgaatggt acgcacgtta gacgtgatat
     4381 cttccccggt ggtgccatcg ccacgggtcg cggcactgac taaaacgcca ttttcataca
     4441 gaatactgac agcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgc
     4501 ttttcagacg gtcctgcact cgtttgttga aggcaagaaa gctttcttca tcaaaaacgt
     4561 tatccagcga cagcattggc acttcatggc gaatctggct gaaagccgcc agcggcgcag
     4621 cacctacacg ttgggtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct
     4681 cgcgcagttc gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca
     4741 catgataaag atattcatga tggcgaagcg tcgttcgcag ttctgtcagt tgttgttcga
     4801 ttgattccat atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga
     4861 gttaatttgc cttaagtgta tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg
     4921 tactcgcgca atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc
     4981 acttcatcgg caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca
     5041 ccgtaagacg gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca
     5101 ggatcaaagg ttcccggttt caccatattc gccaggctga ataacgccgg gccgctgcca
     5161 tccgggctaa gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga
     5221 atgctgttaa gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg
     5281 ataatcaccg cttctttgcg cttcggttta tccataactg gagccggttc cgctacaggc
     5341 tcaggctgtg gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt
     5401 gctgaatgca caggctgcgg cgctggcgcg acctgtggcg aaactggctg ctgcaacggc
     5461 tgttcaggct gcggctgata ggcaggctgc tgcaccggct gcgctggacg cggagcatgt
     5521 tgcggcggta cctgcgcttc aggcggctgc tggaccggtt gacgcggctg cgcagacgca
     5581 taaggcggtt ggtactggtg ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta
     5641 gccggggcat gattcacgcg gtgaacacga acctcaccaa cgccctcatc atcttcgaca
     5701 tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg
     5761 aacatagaag atcgttcttt acggctggtc cagaaaccat gtaccagtaa agcgattatg
     5821 gcgatcgcgc caacaatgat taatatcaga cgcaaatcct gcatcattat attctctgtt
     5881 gttctaacac cttgccacca cggcaaacat ttactcacta agagtatttg ccgattacct
     5941 caagtgcaag tgcactatta actttcacag cacaaagata gatgaaatcg tgctttttgc
     6001 tgttttttcg aacatatcct aactgtccat tgcgcaatta cccggtaaaa tacgcagaat
     6061 tttcctggga ttggtcaaaa ggagctcatc ctgattatgg tttcatcatt cacatctgcc
     6121 ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt
     6181 cggcgtttcg ttattttacc gctgctggtc aatattttgt tgatgggggg cgcattctgg
     6241 tggctcttta cgcaactcga tgtctggatc ccgactctca tgagttatgt tccggactgg
     6301 ttacaatggc tgagctatct gttgtggccc ctggctgtca tctctgtgct gttagtgttt
     6361 ggctatttct tctcaacgat tgctaactgg attgccgctc cgttcaacgg tttattggct
     6421 gaacaactgg aagcacgatt gactggcgct acaccgccag ataccgggat tttcggtatc
     6481 atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa aatttgcctg gtatctgcca
     6541 cgcgcaattg tattgctaat tctttacttc atccctggta ttgggcaaac cgtcgcgccg
     6601 gtactgtggt tcctgtttag cgcctggatg ttagccatcc agtattgcga ttaccccttc
     6661 gataaccaca aagtgccgtt taaagagatg cgcaccgccc tgcgcacacg caaaatcacc
     6721 aatatgcagt ttggtgcttt aaccagtctg tttacgatga tcccgctgct taatctgttc
     6781 attatgcccg ttgccgtttg tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa
     6841 cacgcgatgt ggcggtaaca atctaacggt tattttgtaa accgtttgtg tgaaacaggg
     6901 gtggcttatg ccgcccctta ttccatcttg catgtcatta tttcccttct gcatatagat
     6961 atgctaaatc cttacttccg catattctct gagcgggtat gctacctgtt gtatcccaat
     7021 ttcatacagt taaggacagg ccatgagtaa gatttttgaa gataactcgc tgactatcgg
     7081 tcacacgccg ctggttcgcc tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga
     7141 atctcgtaac cccagcttca gcgttaagtg ccgtatcggt gccaacatga tttgggatgc
     7201 cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt gaaccgacca gcggtaatac
     7261 cgggattgca ctggcctatg tagctgccgc tcgcggttac aaactcaccc tgaccatgcc
     7321 agaaaccatg agtattgaac gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct
     7381 gacggaaggt gctaaaggca tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag
     7441 caatccagag aaatacctgc tgctgcaaca attcagcaat ccggcaaacc ctgaaattca
     7501 cgaaaaaacc accggtccgg agatctggga agataccgac ggtcaggttg atgtatttat
     7561 tgctggcgtt gggactggcg gtacgctgac tggcgtcagc cgctacatta aaggcaccaa
     7621 aggcaagacc gatcttatat ctgtcgccgt tgagccaacc gattctccag ttatcgccca
     7681 ggcgctggca ggtgaagaga ttaaacctgg cccgcataaa attcagggta ttggcgctgg
     7741 ttttatcccg gctaacctcg atctcaagct ggtcgataaa gtcattggca tcaccaatga
     7801 agaagccatt tctaccgccc gtcgtctaat ggaagaagaa ggtattcttg caggtatctc
     7861 ttctggagca gctgttgccg cggcgttgaa actacaagaa gatgaaagct ttaccaacaa
     7921 gaatattgtg gttattctac catcatcggg tgagcgttat ttaagcaccg cattgtttgc
     7981 cgatctcttc actgagaaag aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa
     8041 aagcaccttt ttaggtgctt ttttgtggcc tgcttcaaac tttctcccct cctggcattg
     8101 attcagcctg tcggaactgg tatttaacca gactaattat tttgatgcgc gaaattaatc
     8161 gttacaggaa aagccaaagc tgaatcgatt ttatgatttg gttcaattct tcctttagcg
     8221 gcataatgtt taatgacgta cgaaacgtca gcggtcaaca cccgccagca atggactgta
     8281 ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct aacaatacag gctaaagtcg
     8341 aaccgccagg ctagacttta gttccacaac actaaaccta taagttgggg aaatacaatg
     8401 ttccagcaag aagttaccat taccgctccg aacggtctgc acacccgccc tgctgcccag
     8461 tttgtaaaag aagctaaggg cttcacttct gaaattactg tgacttccaa cggcaaaagc
     8521 gccagcgcga aaagcctgtt taaactgcag actctgggcc tgactcaagg taccgttgtg
     8581 actatctccg cagaaggcga agacgagcag aaagcggttg aacatctggt taaactgatg
     8641 gcggaactcg agtaatttcc cgggttcttt taaaaatcag tcacaagtaa ggtagggtta
     8701 tgatttcagg cattttagca tccccgggta tcgctttcgg taaagctctg cttctgaaag
     8761 aagacgaaat tgtcattgac cggaaaaaaa tttctgccga ccaggttgat caggaagttg
     8821 aacgttttct gagcggtcgt gccaaggcat cagcccagct ggaaacgatc aaaacgaaag
     8881 ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga agggcatatt atgctgctcg
     8941 aagatgagga gctggagcag gaaatcatag ccctgattaa agataagcac atgacagctg
     9001 acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc cctggaagag ctggatgatg
     9061 aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca
     9121 tcctgggcct gaagattatc gacctgagcg ccattcagga tgaagtcatt ctggttgccg
     9181 ctgacctgac gccgtccgaa accgcacagc tgaacctgaa gaaggtgctg ggtttcatca
     9241 ccgacgcggg tggccgtact tcccacacct ctatcatggc gcgttctctg gaactacctg
     9301 ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa tgacgactat ctgattctgg
     9361 atgccgtaaa taatcaggtt tacgtcaatc caaccaacga agttattgat aaaatgcgcg
     9421 ctgttcagga gcaagtggct tctgaaaaag cagagcttgc taaactgaaa gatctgccag
     9481 ctattacgct ggacggtcac caggtagaag tatgcgctaa cattggtacg gttcgtgacg
     9541 ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct gtatcgtact gagttcctgt
     9601 tcatggaccg cgacgcactg cccactgaag aagaacagtt tgctgcttac aaagcagtgg
     9661 ctgaagcgtg tggctcgcaa gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag
     9721 agctgccata catgaacttc ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc
     9781 gtatcgcgat ggatcgtaaa gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct
     9841 cggctttcgg taaattgcgc attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg
     9901 cactgcgcaa agagatcgaa atctacaaac aggaactgcg cgacgaaggt aaagcgtttg
     9961 acgagtcaat tgaaatcggc gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc
    10021 atttagccaa agaagttgat ttctttagta tcggcaccaa tgatttaacg cagtacactc
    10081 tggcagttga ccgtggtaat gatatgattt cacaccttta ccagccaatg tcaccgtccg
    10141 tgctgaactt gatcaagcaa gttattgatg cttctcatgc tgaaggcaaa tggactggca
    10201 tgtgtggtga gcttgctggc gatgaacgtg ctacacttct gttgctgggg atgggtctgg
    10261 acgaattctc tatgagcgcc atttctatcc cgcgcattaa gaagattatc cgtaacacga
    10321 acttcgaaga tgcgaaggtg ttagcagagc aggctcttgc tcaaccgaca acggacgagt
    10381 taatgacgct ggttaacaag ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg
    10441 gcccaattta ctgcttagga gaagatcatg ggtttgttcg ataaactgaa atctctggtt
    10501 tccgacgaca agaaggatac cggaactatt gagatcattg ctccgctctc tggcgagatc
    10561 gtcaatatcg aagacgtgcc ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt
    10621 gctatcaaac caacgggtaa caaaatggtc gcgccagtag acggcaccat tggtaaaatc
    10681 tttgaaacca accacgcatt ctctatcgaa tctgatagcg gcgttgaact gttcgtccac
    10741 ttcggtatcg acaccgttga actgaaaggc gaaggcttca agcgtattgc tgaagaaggt
    10801 cagcgcgtga aagttggcga tactgtcatt gaatttgatc tgccgctgct ggaagagaaa
    10861 gccaagtcta ccctgactcc ggttgttatc tccaacatgg acgaaatcaa agaactgatc
    10921 aaactgtccg gtagcgtaac cgtgggtgaa accccggtta tccgcatcaa gaagtaattc
    10981 ttgccgcagt gaaaaatggc gcccatcggc gccatttttt tatgcttccg ccagcggcgg
    11041 caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa
    11101 ccccgctcgg tgcactgcat cggttaacgc cttccctttc agcaagccac tgatgagctg
    11161 agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat gggaaatgac
    11221 attcacgctg tcggcagtga ccaccacaac ctgcatctcc tgattttctt cattaccgga
    11281 ggcgctggta accaccaccc attttaatgt gtctgaaagc agactttttg cggcagcaat
    11341 ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccaactcaa agatattggg
    11401 ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcttcgggaa ggtcaggttt
    11461 gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca ataggtcagg
    11521 atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg atgccgttcc
    11581 catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct gaagcgcacg
    11641 caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt cataatgcgg
    11701 cgtattgctc agcaataccg tcggcacggc aaagacattc aggccgttct gtttgatagc
    11761 aggcacggca atgctgttgc ccacgctgcc gtaaaccacc tgcgactgca cggcgacgat
    11821 atccgcctgc agtgccctac tcttatcgtt aaacaacaac aaactactca tttaattttt
    11881 tctccttgcc gatgatcctc atcgtaatcc aaccgaaact ttacctgatt ctggcagtca
    11941 aatcggctat cacaaaacaa ggataaggta attcaatgaa gaaaatcatt tgtctggtca
    12001 ttacactact aatgacactc cccgtttacg cgaagttaac tgcccatgaa gaagcccgca
    12061 tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg cgcaacggtg
    12121 atgaacatac ctgctatgaa gcggtttctc atctgcgtct gaagctcggc aatacccgta
    12181 accgcattga cactgccgag cagtttattg ataaggttgc ttcgtcgtca tcgattactg
    12241 ggaagccgta tattgtgaag atccccggta agagcgatga gaacgcacag ccttttttac
    12301 atgcgttaat tgcgcagacg gataaaacgg tgcctgcgga aggaaattaa tccgctttgg
    12361 gaaggcattt acaggaggta acatgaaaaa acgctttatt tatcacgatg aaaaatcgaa
    12421 taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg gcaaggtagg
    12481 tagtattggt aaattccaga caaaagagtt cgataatgaa gaacagtgtc tgaaagaagc
    12541 cagtaaattg attgccgcaa aaatgaagaa aggctatcaa gaagatccaa agtttaactt
    12601 catggatcgc tactattttg atgatgaaga aattgggtta catgttaaaa cgtcacaccc
    12661 aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg aagaatctcc
    12721 ttttggcagc gatgaaggtg ctgatgctct aaacgttctt gaaaatagcc tccgtaaaga
    12781 gccggatctg gactgtgctg atttccctca aatgttaatt gaaactatgt ggggtatgaa
    12841 atacatcgct atggacagta ttcttgaaga ggatgttcgt gcgcaattac tagtcgatga
    12901 aatgagcact atccagagca atatgattac ctacgcaact gcattcggtc agattaaagt
    12961 catgggtaaa atctcccata aacttaaaaa gatgggactc aatgcactag cgcgtcatca
    13021 gcttaccgca aaaattcttc aatggggtga cggtcaggac tcaccaatac ttcaaaaaat
    13081 gattgatgac cttacggcgt ttcctcacga aaattaaata ctgcatttgt cggcagcaac
    13141 aactgttaaa aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    13201 acaaaatcgt gctaattcaa tatattgtag aaaccttgta ggcctgataa gcgtagcgca
    13261 tcaggcagtt ttgcgtttgt catcagtctc cgatgctatt aatccttaaa tccccgcccc
    13321 ctggctaaag tgctcttccc caaacacccc ggtggaaagg tagcgatcgc cacgatcgca
    13381 gatgatcgcc accaccaccg caccagggtt agcttttgcc acccgcagtg ctccggcaac
    13441 cgcgccgccg gagctgacgc cacagaatat tccttcccgc accgccagtt cgcgcatggt
    13501 gttttccgca tcgcgctgat gaatatccag cacctcatcc accagagaag cgttgaaaat
    13561 ccccggcaga tattccgcag gccagcggcg aatgccggga atgctactgc cctcttccgg
    13621 ctgcaggcca acaatggtca ccggtttgga ttgttcgcgc ataaagcgtg aaacgccggt
    13681 gatagtgccg gttgttccca tgctggagac aaaatgggta atgcgcccgc cagtttgctg
    13741 ccagatttcc ggcccggtag tggtgtaatg ggcgtaaggg ttatcgggat tattgaactg
    13801 atcgagcagc tttccttcac cacgattagc catctccagc gccagatcgc gcgcaccttc
    13861 catgccctgc tctttggtga caagaatcag ttccgcacca taagcacgca tcgccgcacg
    13921 gcgttcctgg ctcatgttgt cgggcatcag caatttcatg cgatagcctt taagcgcggc
    13981 aatcattgcc agcgcaatgc cggtgttacc actggtggct tcgattaaca catcacccgg
    14041 tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa agcgccgcac gatcttttac
    14101 cgaacctgcc gggttattgc cttccagttt taaccacact tcactgccgt tattcggccc
    14161 cattcgctgc aacttcacca gaggcgtatt gcctattgtt tgttctaatg tactcacgat
    14221 ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc gtcttatcag gcctacaggt
    14281 tacaaacctt gccattaaaa agcccggatc gcgggaagcg cctccgggcg tttaacattc
    14341 actcaaccta tcaggcgctt tgtgcgagag caagttcctc atcgcgggtt tcgatacgct
    14401 cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa taaacgctcg ccacgctgcg
    14461 gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt gtaccacccc agcggctgca
    14521 ccactaattg ggtgtagtga cctttcgggc tggcttccag tacctgtacc ggcagcggcg
    14581 aatcgaggct ggtacggcgg ctgatatcca cttcccaagg gcgcaggaag agatccaccg
    14641 gcccctgata cgcaggtgtg tagcccagcg gccagcgatg cgcgccaaca tggaactgcc
    14701 cgccgcgaat ggttccctgc aggcggttca cttcgcccat aaattcgagc acaaaacggg
    14761 tcgccggttc gcgccatacc tgatccggcg cgtcagcctg ttcaatattg ccctggctca
    14821 tcacaactac acgatcagct acttcggtcg cttcttcctg atcgtgagtc acaaaaacgc
    14881 tggtgaattt tagttcttca tggagttgac gcagccagcg acgcagctct ttacgcacct
    14941 gcgcatccag cgcgccaaac ggttcatcaa gcagcagaat ttgcggttcc acagccagcg
    15001 cgcgcgccag cgccacgcgc tgtttctggc cgccggaaag ctgcgccgga taacgatccg
    15061 ccagatgggc aagctggacc atttccagca attttgtcac tttcgctttg atggctgcgg
    15121 cattcgggcg ctcgcgacgc ggcagcaccg tcaggccaaa agcgatattg tcgaacaccg
    15181 tcatatggcg gaacagcgcg taatgctgga acacgaaacc gactttacga tcacgtgcgt
    15241 gcaggcggct cacgtcggtg ccgtggaagc gaatatgccc gctggtttga tgctccagcc
    15301 cggcgataat gcgcagcagc gtggttttcc cggaaccgga cggccccagc aacgcgacca
    15361 tctgacctga aggaatatcc agtgagatat cgttcagcac ctgggtgcga ccaaacgact
    15421 tcttaatatt ggcaatctca atgctcatga tgttcctcct gctgtgcgcg tttttcctga
    15481 ttctccaggc gccactgcaa catacttttt aaaaacaggg tgataatcgc catcagcgtt
    15541 aacagcgccg cagcggtaaa ggagccgacg gtgttgtagt cctgctccag caattcaatc
    15601 tgtaacggca gcgacagggt ttcgccgcga atcgagccgg aaaccaccga cactgcgcca
    15661 aactcgccaa tcgcgcgggc gttggtcagc accacgccat aaagcagcgc ccagcgaatg
    15721 ttcggcaatg tgacgcggcg aaacatctgc cagccagacg cgccaagcaa aattgccgct
    15781 tcgtcttcct ggctgccctg gcttaacatc accggcacca gctcgcgcac cacaaacgga
    15841 cacgtcacga agatcgtgac cagcaccatt ccgggccaag agaacataat ttgcagatta
    15901 tgttcatcca gccagccgcc gagcgggccg ttggagccgt agaacagcag atacaccaga
    15961 ccggcaacca ctggcgatac ggcaaaagga atgtccagta gcgtcagcag taactggcgt
    16021 ccggggaagt taaagcgcgt caccagccag gccagcagaa tgccgaacac caggttcacc
    16081 ggtacggcaa tcagcgcaat catcaccgtc agccaaatcg cgtgcaacat gtccggatcg
    16141 gccagattct gtaaaaccgg catcagcccc ttgctgaatg cctgcacgaa gatgtaaatc
    16201 atcggcacca gcaggatgaa cgccgaaacc agcatcccga tgccaatcag aaaccatttg
    16261 ccccagttaa tcgggcgcgc gtcataacgc ttcaattgtg tcacttccgc cattaatgac
    16321 ctaccacacg ccgaccaaag cgactttgca gagtgttaat tgagaacagc agtatcaggg
    16381 atgccgcgag gatcaccgaa gcaatcgcgc tcgccgccgg gtaatcaaac tcctgcaagc
    16441 gcacaaaaat catcagcgac gtcacttcag tcttccacgc gatatttccg gcgataaaaa
    16501 tcaccgcgcc aaattcacca agactacggg taaacgacag cgccacgccc gccaccagcg
    16561 ccggagaaag ctccggcagc accactttgc agaaactctg ccagcgcgtt gcaccaagtg
    16621 tttccgccgc ttcttcatat tccgggccta actcttccag caccggctgc acggtacgta
    16681 ccacaaacgg aatgctggta aaggccatcg ccaccgcaat ccccagccag gtataggtga
    16741 ctttgatatc aaacttcgcc agccattcac cgtaaaaacc gtttacagaa aagagcgagg
    16801 ccagcgttaa accggcgact gccgttggca gcgcaaaggg taaatccatc agcgcatcaa
    16861 gcagcgtgcg gccagggaag cgataacgag ttaagatcca cgccatcagc agaccgaaaa
    16921 cgccgttaaa aatcgatgcc acaaacgccg acagcagcgt cactttgtag gctgcgacca
    16981 cctgcgggtt ggtgatcacc tcccagtact gcgcccagct catctgggcc agctgcatca
    17041 ccagcgcgga gagtggcagc agcaaaatca ggcaaacaaa cagcagactg gtgccgaggc
    17101 ttaaggtaaa gcccggcagc acgcgtcttg aagaaaccgc aaacattaat tacgccccgc
    17161 cgctaacagc ttgtctaact cgccgccgct ggtgaagtgg gttttcatca cttccggcca
    17221 ggagccaaat ttgtcttcca cgcggaacag ctcggtctgc gggaatttat ctttcagttt
    17281 gtccatgact tccgggttat tcacgcggta gtaatagtcg gtgatgatgg tttgcgcctg
    17341 cgggctgtag agccagttca ggtaggcttt ggcggctttt tccgtaccgt tggcctgcac
    17401 gtttttatcg acccacgcca ccgggaattc cgccagaatg ttggttttcg gaatcaccac
    17461 ttcaaagccc tgcgcttcat actgtttgcg gatgttgttc acttccgact cgaagctgat
    17521 cagcacatcg cccaggccgc gctcggcgaa ggtggtggtc gcgccacgac cgccggtatc
    17581 gaacacttca acgtttttca ggaactgagt cataaactgt tcggttttgg ctttgtcgcc
    17641 accgtcagct ttgtccgctg cgccccatgc cgccagatag gtataacgcg cgttacccga
    17701 cgttttcggg tttgggaaaa tcagcttcac gtcggagcgc accaggtcgt tccagtcgtg
    17761 gatattcttc gggttgccct tacgcaccag gaagcccatg gtggagtaga acggcgagct
    17821 gttattcggc aggcgcgact gccagtcggc cgggatcagc ttgcctttgt catgcaggat
    17881 ttgtacgtcg gtcacctggt tataagtgac aacgtctgct tttaagccct gcaaaattgc
    17941 cagcgcctgt tttgatgacc cggcatgaga ttgttttatc gtcagtttgt caccgccgtt
    18001 atctttggcc cattgttgct caaacggcgg gttcagggcg gcaaacagct cgcgggagac
    18061 gtcataagaa ctgttcagca gttccgttgc ctgtacatgg cccgccagca gcagagaagc
    18121 gaccagcgcg agtgagttct ttttcagtaa gttaacggcc attgcgcacc cttataaatt
    18181 taatgacttt ctaatagcca tcatatttat aacggacgtg aaaggagtaa cggttttata
    18241 taccgtttgg tgatttggaa gttgaaaagg gaataagacg aaatcctccc cacaaatgca
    18301 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    18361 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    18421 ccagttcgcc gacttccagc ggatcggcga ggcgacgcat cgggattgct ttcgccattt
    18481 cagtcagcac cgactctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    18541 gtgtgcgcac gtatcccggg caaatggcgt taacgcgaat accagactgc gcgtactcca
    18601 ccgccagcga ttttgtcagg ccaacaatcg ccgctttcgt taaggcgtac gccgtttcgc
    18661 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    18721 gggcaatcat ctccggcagc accgccttcg tgacgttcca tacaccttta atattgatgt
    18781 caatatggaa atcgcgatcg tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    18841 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    18901 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    18961 cacacagttc gtccgccagc ttttcgatct caggggagat atccagcaag attaggtttg
    19021 cgccatgacg cgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    19081 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    19141 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    19201 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    19261 ctcactgctg cgctgaatca ttttcaatga ctccacatca ttgagttgca caagaccaac
    19321 aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta gacatcgacg agctacgcca
    19381 ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca gcggagagtc
    19441 agccagagaa gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt ctgcgcacaa
    19501 gacaatctct tttttactgc cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa
    19561 cgcctgggaa acggtagctt gtacgtgcgt gtcggcttcg caggccacgc gatagccaat
    19621 tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc gatccgccca ggcccgttat
    19681 ctggataaac ggtgctttac tgatgacctc aatgattttt tgcagccgcg cgtagtcgaa
    19741 caacgcacag gtttgctcca gcgccagctc tttttcgcga ttcaatttgc gggcaatcac
    19801 ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg cattcgtttt
    19861 ctcccggctg gcactgtatt cgccaattaa cgccatacgc aattcagtaa atccttgcgc
    19921 gccgagcttc tgggcaaatt tcacaatact cgactggctg atgccgagct gtttcgccat
    19981 cttgcgcgaa gagacggatt tcagctcact cacgttggcc cgcagaaagt cagcaatttt
    20041 ttgctcgttc ccggtaaatt cactttccgc attgcgtatt ttcgtcaggt acaacatctt
    20101 ccactctcct gggctggcag caaaaattcc gcttaagttc ttatgacgct cttcacactc
    20161 tgcgagtgta gactcaatga ttcctttaag tctgtcaatt cgggatatat gattccatac
    20221 atctgcaatt atttgatcgt aaatagtaag gtcaccaccg atgcaatttg aaaagatgat
    20281 tactgaaggc tcgaacaccg cctcggctga aattgaccgc gtatcgacgc tggaaatgtg
    20341 ccggattatc aacgatgaag ataaaaccgt accgcttgcc gttgagcgcg tactgccgga
    20401 tatcgccgcg gcgatcgatg ttatccacgc ccaggtaagc ggcggcgggc gtctgattta
    20461 cctcggtgcg ggaacatccg gtcgtctggg gattctggat gccagcgaat gtccgcccac
    20521 ctacggcgtg aaaccgggtc tggtggttgg tttgattgct ggcggcgaat atgccattca
    20581 gcacgcggtg gaaggcgcgg aagatagccg ggaaggcggt gttaatgatc tgaaaaatat
    20641 taatttaacg gcacaggatg tggtggttgg cattgctgcc agcggtcgca cgccgtatgt
    20701 gattgccgga ctggaatacg cacgccagct cggctgccgc acagtgggaa tttcctgtaa
    20761 tccggggagc gccgtttcaa ccaccgctga gtttgccatt acaccgattg taggtgccga
    20821 agttgttacc ggttcttcgc ggatgaaagc aggtacagcg cagaaactgg tgctcaatat
    20881 gctttccacc gggctgatga ttaaatccgg caaagtgttc ggcaacctga tggtcgatgt
    20941 ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat attgttaaaa acgccaccgg
    21001 atgtagcgca gagcaagcgg aagcggcgtt aattgcttgc gagcgcaact gtaaaacggc
    21061 cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca
    21121 cggcggcttt attcgtcagg ttttagacaa ggaataaccc atggccaaag agatcagcag
    21181 tgaacttctg aacaccattc ttacccgtgt cggcggaccg ggaaatatcg ccagttgtgg
    21241 taactgtatg acgcgcctgc gtctgggtgt acatgacagt tcactggttg atcccaatat
    21301 caaaacgctg gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc aggtcgtttt
    21361 tgggcctggt aaagcacatc gtgccgcaaa agcgatgagc gagctgctgg gcgaggcacc
    21421 ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac
    21481 ctccggcgtg caacaatttc tcgccaaatt cgccaccatc ttcacgccgc tgatccccgg
    21541 ttttattgcc gccggtctgc tgctggggat agcgacgtta attgccacgg tgatgcacgt
    21601 tccggcagat gctcagggaa cactacccga tgcgctgaat tttatgaagg tgttcagcaa
    21661 aggtttgttc actttcctgg tgattctggt gggctataac gccgcccagg catttggcgg
    21721 cacgggcgta aatggcgcaa ttatcgccgc gctctttttg ctcggttata accccgcagc
    21781 gaccaccggt tactacgccg gttttcacga tttctttggt ctgcccatcg atccgcgcgg
    21841 caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag gcatggtgcg
    21901 ccgctttatg ccggacgatc tcgacatgct gctgacctcg ttaatcaccc tgctgatcac
    21961 cgctacgctt gcgtacctga tcattatgcc gctgggtggc tggttattcg aaggtatgtc
    22021 atggctgttt atgcacctga acagtaatcc gttcggttgt gcggttttag ccgggctgtt
    22081 cctgatcgcc gtggtatttg gcgtgcatca gggctttatt cctgtttacc tcgcgttaat
    22141 ggacagccag ggattcaaca gcttatttcc catcctttca atggcaggcg cgggccaggt
    22201 gggcgcggcg ctggcactct actggcgggc gcaaccgcac agtgcgctac gcagtcaggt
    22261 acgcggggcg attattcccg gcctgctggg cgttggtgaa ccgctgattt acggtgtcac
    22321 cctgccccgc atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg gcggtttgtt
    22381 tatcggcctg atagcctggt ggggtctgcc gatgggctta aacagcgcct ttgggccgtc
    22441 tggtctggta gcgctgccgc tgatgacttc cgcacaaggc atccttccgg ccatggcggt
    22501 ttatgctggc gggattctgg tggcatgggt ttgcgggttt attttcacca cgctctttgg
    22561 ctgccgtaac gtcaatctgg actgatatca tgaaacggac aatgctctat ctttctctgc
    22621 tggctgttag ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa agctcgccag
    22681 agaaagcagg gtttaacgtc gaacggctta accagatgga tcgctggatt agccagcaag
    22741 ttgatgtcgg ttatcccggc gtaaacctgc tgatcattaa agataatcag attgtgtatc
    22801 gcaaagcctg gggagcggcg aaaaagtacg atggcagcgt gttgatggaa cagcctgtca
    22861 aagccaccac cgggacgctg tatgacctgg cctcaaacac caaaatgtac gccacgaact
    22921 tcgccctgca aaagctgatg agcgaaggca aactgcatcc tgacgatcgg attgcgaaat
    22981 atattccggg gtttgccgat agtccaaatg acaccattaa aggcaaaaac accctgcgga
    23041 tttctgacct gctgcatcac agcggcggtt tcccggcaga tccgcaatac ccgaataaag
    23101 cggtcgcggg cgcgttatat taccaggata aaggccagac gctggagatg atcaagcgca
    23161 cgccgctaga gtatcagccc ggcagcaagc atatttacag tgatgtcgat tatatgctgc
    23221 ttggatttat cgtcgagtcg gtcaccggtc agccgcttga ccgctatgtt gaagagtcga
    23281 tttatcgccc gctcggcctg acacatacgg tatttaaccc gctgctgaaa ggctttaaac
    23341 cgcaacaaat tgccgccacg gaattgaacg gcaatacccg cgatggcgtg atccatttcc
    23401 cgaatatccg cacctccact ctctggggtc aggtgcacga tgaaaaagcc ttttattcga
    23461 tgggcggcgt ttccgggcac gcaggtttgt tttccaatac cggcgatatt gcggtgttaa
    23521 tgcaaacgat gctgaacggc gggggctatg gtgatgtgca gctgttcaat gcggaaacgg
    23581 tgaagatgtt caccaccagc tctaaggaag atgccacttt tggcctcggc tggcgcgtga
    23641 atggtaatgc caccatgacg ccgacgtttg gcacgctggc aagcccgcaa acctacggtc
    23701 acactggctg gaccggaacg gtgaccgtta tcgatccggt gaatcatatg gcgattgtga
    23761 tgttaagcaa caagccacat tctccagttg ccgatccgca aaagaatccc aatatgttcg
    23821 aaagcggtca gttgccgatt gcaacttatg gttgggtagt tgatcaggtg tatgcggcgt
    23881 taaagcagaa gtaataaaaa ggccagtcag taatgttact ggctggtctg agagtgctga
    23941 caaacgcaaa actgcctgat gcgctacgct tatcaggcct acgtggagcc cacaatatat
    24001 tgaattagca cgatttcgta ggccggataa ggcgttcacg ccgcatccgg cgtgtacaac
    24061 gcgcacttgg tcaacaatct gaggccagtc agtaatgtta ctgcctggcc tttaatcaat
    24121 gaatcagaaa cgcttacagc gccatcaact tgtccagcga cggtgcgaaa taatagccgc
    24181 cggttaccgg tttggtgaaa cgcaacatcg catcacgctt accatcggta tcgccaaaca
    24241 tgctcagcag ttgctgctca atgttatgca gacgcgcgca gtaggcgcag aagtacagac
    24301 cgtgagtgcc actggcggtg ccgtacggca ggctctggcg aacaatcttc agccctttgc
    24361 catcttcttt cagatcaacg cgggtgaggt gagaggtttc cggacgttcg tcgccgtcga
    24421 tctcttcgtt atcttctttg gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc
    24481 ggttgagctg cttcagattg tgctcccaac gctgcacaaa cacatagctg ccgcccgcat
    24541 ccacgccgtc tttgataacc gccacttcgc ggcgcgtctc ttcacccgcc gggttttcag
    24601 taccgtcaac gaagccgctc aggtcacgct cttcaaccca acggaagccg tggatctctt
    24661 ctttcacttc aatgcagtca ccaaaggctt ccatcgccgc ctgggcgaca gagaagttta
    24721 cgtcgtgacg cagagaaaga atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc
    24781 ctttaccgta gcccggaaaa tctttcagct cttctgcccc aacgccgccg ctcagagcgc
    24841 gccaggtgtt gttaccaaag gcaaccaccg caccaagatg cgcgtccggg aatttcgctt
    24901 caaaagttgc cagtttgtcg gcaaatgttt tactggccgc acgcagggcg tcaacttccc
    24961 ctttcacgtt ggcttcgatc caaatcgccg cgcggcaatg ttctggcaaa atgccactct
    25021 gaacctgaga cattgttcct cctgaaaata ataatgccac gcaatccgtg gcattgttga
    25081 gcgctatttt acgtgttttt tcgcggcggc ggtttgttca gacgcaaatt aacgccgcca
    25141 gataatttta ctgactttcc agtttttcag ggtatcgtcc gaaggcatta acccttcagg
    25201 accacgccat tccccgctga actggtagct aatatgttga ctgccttcgg ctttacattc
    25261 gacggcacga ttatcatcac catcagcttt ttggcaattg ccaaatgctt tgctgtaaag
    25321 gtcgctaaac ggtgtaccga ttttaacacc ggtgtcagca ggaatatcgc tatccagcac
    25381 gtcaatgcga ctgatcgtgc cttgatcgcc gttaatcacc atcgccacgt tgtcgccctt
    25441 catcacttca aaaaagcgca ccacgttgcc gttcgcggat ttcattccgc tgcgcaggcg
    25501 ataatcgcca tcaagcgcat cggcaatggc ttgttcttgc agtggtgtgg acgccgttaa
    25561 ttcacccacg ccctgctcgc tcactttggt ggacgatcca aaccagttcc acgggttagc
    25621 ggcagaccag ttaactgacg acatcgtgga acagccggtc agcatcaatg gcatagcgca
    25681 taacattaaa cgcagcgatt tcatgtcact tcctttggtt attcaataac gttgcttgga
    25741 gtgcaaattc accaaaaagt gccgttattc tttttcttca ttaaaacaag cgcgtaaacg
    25801 tcgattggtc agcagccaga ttaacgccac aatatccgcc actaccagcg ccagaccgat
    25861 accgctaacg gattcaccgt tcagccacag ccacggttgc cagcaaagta aaaccacctg
    25921 cgccaacagc aacagaaaat agagtacacg ccaggtgcga ggaaacgtag cccgccgacc
    25981 gctcaacaaa aacgccagca ccgccggaat gccaggaatc agccccagcc agaaattatc
    26041 gtgatcggga taaaacagat ttaacagcgc agtaccctgc tcgcgcgacg caccggcaat
    26101 gacaaacagc acccaggttc gcgcctgaag caatagcaca agccagaaga gcaagggtaa
    26161 acgcaggcga ccgtgcgcat cataatggac aggatgaaac tcagtactct tcatcttcaa
    26221 tcaaacgctt acccagactc agcacgtcgg cgtgttcata tcccaggcgt tcatacattc
    26281 cgagcaccat gtcgttatct tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa
    26341 tcagcttttt ctccagccgg ttaagcaacg cattggcaat tccacgccca cgaaactctg
    26401 gatgcacgcc aagataataa gcagacccgc gatgcccgtc ataaccgccc atcaccgttc
    26461 cgaccacttc accgtttacc tcagcgacca gaaacaaact gacgtcatgg ttcatcttac
    26521 gctcgatgtc catttccgga tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg
    26581 tgatgacctc ttcgaaatct tcctggcgaa atacgcgtat ctccatggta ttcgttacct
    26641 ttttgcgggt taaaaggctg attatggcgt gaacggtcga attagccaat atctgacgaa
    26701 aatcggttga aaaagtggca taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca
    26761 attctcaaca tcaaaccgtc gtaacggatt acgcgatacg atataacatc tggaacttta
    26821 ttattacaac tcaggccgta tgagcacttt taaatcacta aaaacactca cttcgcgccg
    26881 ccaggtgctg aaagccggtt tggctgccct gacgttgtca ggaatgtcgc aagccatcgc
    26941 caaagacgaa cctttaaaaa ccagcaacgg acacagcaag ccaaaagcca aaaaatctgg
    27001 cggcaaacgt gtcgttgttc tcgatccagg ccacggcgga attgataccg gagcgatcgg
    27061 acgcaacggt tcgaaagaaa aacatgtggt gctggcgatt gctaaaaacg tccgttccat
    27121 tttgcgtaat catgggattg atgcgcgttt aacgcgttct ggcgatacgt ttatcccact
    27181 ttacgatcgc gttgaaatcg cccataaaca tggcgcagat ctgtttatgt caattcatgc
    27241 cgatggcttt accaacccga aagcttccgg tgcttcggta tttgccctct ctaaccgtgg
    27301 ggcaagtagc gcaatggcga aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc
    27361 cggtaaaaag gcgactgaca aggatcacct attgcagcaa gtgctgtttg atctggtgca
    27421 aaccgatact attaaaaaca gcctgacgct cggctcgcat attctgaaaa agattaagcc
    27481 ggtgcataaa ctgcatagcc gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc
    27541 gtcggttcct tcggtgctgg tggaaacctc gtttatcacc aacccggaag aagagcggct
    27601 gttaggcacg gcggcgtttc gtcagaaaat cgccacagcg attgctgaag gcgtgatcag
    27661 ttatttccac tggttcgaca accagaaagc acattcgaaa aagcgataag ttatgaaacc
    27721 cgacgcacac caggttaaac agtttctgct caaccttcag gatacgattt gtcagcagct
    27781 gaccgccgtc gatggcgcag aatttgtcga agatagttgg cagcgcgaag ctggcggcgg
    27841 cgggcgtagt cgggtgttgc gtaatggtgg tgttttcgaa caggcaggcg tcaacttttc
    27901 gcatgtccac ggtgaggcga tgcctgcttc cgccaccgct catcgcccgg aacttgccgg
    27961 gcgcagtttc gaggcgatgg gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc
    28021 caccagccac gcgaatgtgc ggttttttat tgccgaaaaa ccggatgccg atcccgtctg
    28081 gtggtttggc ggtggcttcg acttaacccc attctatggt tttgaagaag atgctattca
    28141 ctggcatcgc accgcccgtg acctgtgcct gccatttggc gaagacgttt atccccgtta
    28201 caaaaagtgg tgcgacgaat acttctacct caaacatcgc aacgaacagc gcggtattgg
    28261 cgggctgttc tttgatgacc tgaacacgcc agatttcgac cgctgttttg cctttatgca
    28321 ggcggtaggc aaaggctaca ccgacgctta tttaccaatt gtcgagcgac ggaaagcgat
    28381 ggcctacggc gagcgcgagc gcaatttcca gttatatcgt cgcggtcgtt atgtcgagtt
    28441 caatctggtc tgggatcgcg gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc
    28501 tatcctgatg tcaatgccgc cactggtacg ctgggaatat gattatcagc caaaagatgg
    28561 cagcccagaa gcggcgttaa gtgagtttat taaggtcagg gattgggtgt aactccctca
    28621 cccccactcc cgcatccgct gatgcagcgt cagtgacggc ttctcggaaa acagctgctg
    28681 gtaatccgtg gcaaattgcc ccagatgcca gaatccccac tgcatggcgg cgtcttttac
    28741 cgtcatactt tgcgaccacg gacttatcag ttcgcggcgt acggcgttca ggcgaatgcg
    28801 tttcagccac gcgttcgggc caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg
    28861 gcggctgaca tgcagttgat tacacaaatc cagcaccgtc accggttcgg acatgttttc
    28921 cagcacatat tcacgggcgc gggaaagcaa tcgacggtaa ctctgatgac tgatgctttc
    28981 cgccgtcacc attggttgcg cttcttccag catggccccc atcgccatta gcaaattatc
    29041 ccccagcact tttcgcactg caggctgatg gagattttcc ggattctcgc aaaacgtcgc
    29101 cagcgcctgt tggacaaagc cccacagcgc ggctttatgc tgctctttca cttccagcgc
    29161 cgactggtta cgcaacatat gtaatacccg atccgggtta tgcaaaaagt tagcctgccg
    29221 ggtgatgaca tcttcagaaa gcaccacgcc caggatcgtg taatcatccg gcgtgctcag
    29281 ttcaaattca gtgccaccag ggcgggtggc gatttccgcg cttcccagac attgcgaacc
    29341 gataaatccc tgctcaccgc gcgtcgccgg aatgccaaac cagaacgagt tcggccagac
    29401 caggcacgac tgacgcagcg ccagaccggt gtattcacga aaaacctgaa tatcatcgag
    29461 tagaatttcc gtaaattcac catgaaactt gcccggatgc agctgatcgt aaatctgctg
    29521 ccaggcggta atcgttaaag catgttcata gacatccgtt gtctgtcgtt gatgaacatt
    29581 atccacttcg accttcggcg tgagcttcag gttttcgggt aggggagttt acaaccaagc
    29641 ggcctatctc aaaatgcgcc gtcaaatgct tcaggactac gccgacgcga ttgattcgat
    29701 actggctgat ggtatcaatc cgcttgaacc gtaatgaatg attaatcacg ttttccgacg
    29761 actccaccgg agtaggccgg ataaacactg gacaccttca aaataactat tcacttttgc
    29821 cggattcgtc cggctttttt tgtctttgcc gatgggatca attattccaa ttattccaat
    29881 tattccagta ttgttggaat aaatgaataa atatcaatga cttaaagccg tgtttgcaac
    29941 agggtggatc ggggatcgaa aataggatca acacaaaaac accctggagg ctacagcaca
    30001 ccagggggac acaaaaaaca acacaggaaa aaaataccat gtaccaatca gcaaccgcct
    30061 acgtggcgaa accaggcgaa acactggtca aacacaaaat tgcaacactc caagcacttc
    30121 aaacactcca agcccttccg gaacaggttc aggccgcctt tcgtgatggc atgaacatca
    30181 tccgcttcga tgggtatcgc tgggaataca agccagcaac tggattcgtg cgcatagatc
    30241 aaatcttgtc gccagccaat gaagagccag cgctagccaa tgcagaacca gcgcaggagc
    30301 aggaacagaa taaagcggcg gctattgccg agttcaagga gctgcaaaga caacaaatca
    30361 cagcgcagca agaagaatgc cgccacccgc tggcaatgcc caagccagta gcgaaagcca
    30421 acacagacaa gccgacttca tggtttaacg aagaagcgga agaagatttg cgttttgata
    30481 gtaaagagga gcagttgcct gaagagcggg aacaggtgga aacggaagac cagcaaaacg
    30541 attatcgcaa ccttccagcc gtcaataacg ctatggctta cctcttcaaa agcgggctgt
    30601 ttcacaagct gaaaggcgat gagttaaagc tatatacgtg gatggtatcc aagtgcaggt
    30661 atcgcggtaa cgacaaatat gagatttggt attcccgcac caaaatggca aaagcgttag
    30721 gtaagagcgt tgacacgata gatcgccacc tggaagcgtt gagaaagctg ggacttatcc
    30781 aggaaatgcg ccaggcactg aggggcaaat gtggaaaaac ctacagactg cgaatcccgt
    30841 caaaaggcac agcagaagag gcctcaatga cggcacagca gcacgaaaga gaagcaaaag
    30901 tcagctacct ggtacactag gcagcgggtc agcaaagggg ggcaaatgcc ccttttttca
    30961 tgtcagataa tcagatcatt acctcaataa actaataatc acatcgcggt tacaaccagt
    31021 ttgatagtga tagctcaaac ccagacgtgg cgcgggctgc aagcaaaata agccgcaaaa
    31081 atgcgacccc atgtttacta gccgcaaaaa tgcggtccta tttgccagaa ccttttatta
    31141 ttcaattggt tagtacaaga aaaagcgtga ttgctgttca agaagcatcc agaaaggtca
    31201 tgattttgcc atgattttga cgctgaaaaa aataggtggg catttttgcc cactcactag
    31261 ccgcaaattt gcccactcac tagccgcaaa tttgcccact cactagccgc aaatttgccc
    31321 actcactagc cgcaaatttg cccactcact agccgcattt ttgcggccca aagtaataaa
    31381 tacagagaag taataaatac agagaaataa taaatacaga tcagcgccaa gatttttcgt
    31441 cttttcggcg gaatcatgag actagtgctt acgtcctgat aacctccaga gcagacctta
    31501 acttctgtgc agcaggacta cgctcattgc cgctcggcta ggtcaaatcc tcacgataaa
    31561 gctgttcgga ttgtgaccat aatgttaaag gactctttaa atcattcctt gacgaattaa
    31621 ctagctggct taagcggaat ag
//
LOCUS       DACAPB010000034        31420 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.034, whole genome shotgun sequence.
ACCESSION   DACAPB010000034 DACAPB010000000
VERSION     DACAPB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.034"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            2521..3075
                     /locus_tag="IT904_001401"
     CDS             2521..3075
                     /locus_tag="IT904_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191206.1"
                     /translation="MNDREFRAMLQASRERNKHNSYAYTNNPTSYELPAFSKSERKSI
                     EAVIRSITPRGRYMPTRKTTKNNLKTFLMSFDSYEQLPSRIEDVIIGTCRSFGRDNYH
                     RKVFYLLRSLDEINSSTVTSHLQRQATRLSHELPSDKYCANLTTICNKVIETINHHVG
                     VGNISLTTSEPDFEFDVYAQAEEF"
     gene            3077..3358
                     /locus_tag="IT904_001402"
     CDS             3077..3358
                     /locus_tag="IT904_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191207.1"
                     /translation="MGNKHTLTRVTKTAFEELNRIKNITGLPHSAVMKLAINRDINEQ
                     DLLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLAMEV"
     gene            3358..3744
                     /locus_tag="IT904_001403"
     CDS             3358..3744
                     /locus_tag="IT904_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191208.1"
                     /translation="MYDFYKHRDHLVRFDGKVWKDVTQTNKPKFTHQQLASRFDIWAE
                     AQKDNGDFEFIRGFDYAYESDLKDAIELMENFNSIKNSDKHKANGNRYDKDLIIDAIT
                     KGQSQSYISEKYGLSRSQYYKIKASV"
     gene            3883..4206
                     /locus_tag="IT904_001404"
     CDS             3883..4206
                     /locus_tag="IT904_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191209.1"
                     /translation="MNNFPLQIFIDTDTAMMIQAFTDVGVSIDFDKLLELMADNAESI
                     SDFIHSVEFNEPRMMLPITDSNMKRLVIEQTNKYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4704..5414
                     /locus_tag="IT904_001405"
     CDS             4704..5414
                     /locus_tag="IT904_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3191210.1"
                     /translation="MTNDLYINGRKIDSFGDWSPQGEHPLHTIQRKEHDERIKLEQEL
                     KMSPKVISFVSPEPQEMPDVCKTEALFNLEREYYPLLKAQRIKLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYKYEDNDGFGTFPENIPDVINNLPDGYRIVKMVKASRGAG
                     QFIFMTDRSDEELCEIAKQNILASRNKQLENVKLYLSRELQAMKELVSEYESQKKIAM
                     QADIEQLVKISQKYAKAL"
     gene            5439..5642
                     /locus_tag="IT904_001406"
     CDS             5439..5642
                     /locus_tag="IT904_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191211.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSSTSKQKNRIRRIAK
                     WCLDKLPSDCQIVGEDGNYYLVR"
     gene            5849..7978
                     /locus_tag="IT904_001407"
     CDS             5849..7978
                     /locus_tag="IT904_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP3191212.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQRVEKAALQSAQRIEKNLNKG
                     FRINTKQLDSNLTASLGQLERKFNKAFDKIEQRARNTKAFQFTTRFNDTVNPPKQPRQ
                     PRISGNRAITAAYSANMSKLKGLNPIVEKYIKSQFYGLSAKAGSMDSSKFNEKLAQLN
                     ASVREAIAKARGHTSTSINGDRAANSLDNLSSAAVKTAGAFISMQAVLASYQKIMEIG
                     LQRASAERSIDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQG
                     SEELFKNMIGYARLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVF
                     AKATGKSEQELFDAMKKGALKSSDTLQKVTQELNKQITAKGGWKAISESTQAQLGNLK
                     NSWNTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKSLGESLGSLMTSMSHGVDSLT
                     DISYKVRAFFDEVTLAYRSLNDTQRKIVDGLSDGLIDGLKMLAVVVTAQKAANVAGGL
                     LNLANAVTSFGDAVNKAKAGKAGSSGGALSKVGRVVLYAELVNFALDNLNPEAAKNPN
                     LHQSGIGTETNFNPEYKGDSKPNQINEGWAKIGSSIADWFNGAANKIANLQEPMSNLV
                     NNTQSTPPAITGDMVNSNAQLAMAVSELGRKVDALTAEAKKPLSVNIKGEAIVRPDEP
                     SFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            8143..8913
                     /locus_tag="IT904_001408"
     CDS             8143..8913
                     /locus_tag="IT904_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191213.1"
                     /translation="MIFSNQIRYSGKDGIYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLENWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTSTTNDGGATSKKNTGNTTTNLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSV
                     KNGQSTFTDSANSGAAKVLKAGNALRKFSVNPNKKGTY"
     gene            complement(8946..10466)
                     /locus_tag="IT904_001409"
     CDS             complement(8946..10466)
                     /locus_tag="IT904_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3191214.1"
                     /translation="MRSVITYLRYSSAIQGAEGADSTRRQNDLFKQWLKKNSDAQVVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIESGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGLEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTKMVPGWIDPDTFKLNEHAETVRLIFKLLLSGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDASTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVQPLKRKPFDRWMIDNFLGMIDVGNDGEAEGKIASLQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTMIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEIIVPTDPLPLNDGTSIFEIAEKELLEIDAYQL"
     gene            10557..11276
                     /gene="tonB"
                     /locus_tag="IT904_001410"
     CDS             10557..11276
                     /gene="tonB"
                     /locus_tag="IT904_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAP3191215.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11316..11714)
                     /gene="yciA"
                     /locus_tag="IT904_001411"
     CDS             complement(11316..11714)
                     /gene="yciA"
                     /locus_tag="IT904_001411"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAP3191216.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11819..12358)
                     /locus_tag="IT904_001412"
     CDS             complement(11819..12358)
                     /locus_tag="IT904_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAP3191217.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12388..13131)
                     /locus_tag="IT904_001413"
     CDS             complement(12388..13131)
                     /locus_tag="IT904_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAP3191218.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFNLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(13159..13248)
                     /locus_tag="IT904_001414"
                     /pseudo
     CDS             complement(13159..13248)
                     /locus_tag="IT904_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13488..14126
                     /gene="ompW"
                     /locus_tag="IT904_001415"
     CDS             13488..14126
                     /gene="ompW"
                     /locus_tag="IT904_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAP3191219.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(14186..14692)
                     /locus_tag="IT904_001416"
     CDS             complement(14186..14692)
                     /locus_tag="IT904_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAP3191220.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14738..15238)
                     /locus_tag="IT904_001417"
     CDS             complement(14738..15238)
                     /locus_tag="IT904_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAP3191221.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(15324..15503)
                     /locus_tag="IT904_001418"
     CDS             complement(15324..15503)
                     /locus_tag="IT904_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAP3191222.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(15884..16690)
                     /gene="trpA"
                     /locus_tag="IT904_001419"
     CDS             complement(15884..16690)
                     /gene="trpA"
                     /locus_tag="IT904_001419"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAP3191223.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(16690..17883)
                     /gene="trpB"
                     /locus_tag="IT904_001420"
     CDS             complement(16690..17883)
                     /gene="trpB"
                     /locus_tag="IT904_001420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAP3191224.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(17895..19253)
                     /gene="trpCF"
                     /locus_tag="IT904_001421"
     CDS             complement(17895..19253)
                     /gene="trpCF"
                     /locus_tag="IT904_001421"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAP3191225.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEEQERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLMRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(19257..20852)
                     /gene="trpD"
                     /locus_tag="IT904_001422"
     CDS             complement(19257..20852)
                     /gene="trpD"
                     /locus_tag="IT904_001422"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAP3191226.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(20852..22414)
                     /gene="trpE"
                     /locus_tag="IT904_001423"
     CDS             complement(20852..22414)
                     /gene="trpE"
                     /locus_tag="IT904_001423"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAP3191227.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENSIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(22506..22550)
                     /gene="trpL"
                     /locus_tag="IT904_001424"
     CDS             complement(22506..22550)
                     /gene="trpL"
                     /locus_tag="IT904_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAP3191228.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            22688..23569
                     /gene="yciV"
                     /locus_tag="IT904_001425"
     CDS             22688..23569
                     /gene="yciV"
                     /locus_tag="IT904_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAP3191229.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            23566..24186
                     /locus_tag="IT904_001426"
     CDS             23566..24186
                     /locus_tag="IT904_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP3191230.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            24214..26109
                     /locus_tag="IT904_001427"
     CDS             24214..26109
                     /locus_tag="IT904_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HAP3191231.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMIIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRIFEDGRIESSDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHILLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRLLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLYLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            26347..28023
                     /locus_tag="IT904_001428"
     CDS             26347..28023
                     /locus_tag="IT904_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3258 domain-containing protein"
                     /protein_id="HAP3191232.1"
                     /translation="MYNKRVNAMGNRVDNKAPKFTTKRGNTYYINFRLPDGTFFRRSL
                     GTDSLRAVEVTMSRLSPYIPLVQNGSLSVEQFKMHIDGFREATQRDFDTYLLDWLRLG
                     VDEVKRMPDLGHFQRKLDSDTAISPAATVTEAKGRADIHLNRVYSGDDSTARMLLATL
                     HQKKVSFGQRDLDQVYEVAGQIDMHQAMLHQAYEAFYAGDIVRYRALVDSMQSQLVET
                     ERRKKPATKQAVATVDAPEADNTPLLSEAWKLFVAEKGKNWAITIARENNRFYEVLMH
                     VLGDVPVGSITKQHIRQTLAVIENLPRRNLKPYSEMSLSECIDCDVPEDELISSANVK
                     KHLKIYSSFFKVFLKDEKDILEKSPTEGIKYEVQENKGGHYSKPEMKRLIERLSTYTD
                     WRKDYFLTLIYTGARRGELAAIRKEHIRKDEETGRYYIFIEGGKTEHAQRQIPLSKEI
                     ETLLLARIKPLKSDDLVFGDLPTYEHIALVWRSLMDDCNVPKYNEFGQKRVIHALRHT
                     FISEAIAKTGNAALVQFVVGHSRTQSLGITARYTHRPALSQLLTVVDCIS"
     gene            28081..28371
                     /locus_tag="IT904_001429"
     CDS             28081..28371
                     /locus_tag="IT904_001429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191233.1"
                     /translation="MTASKELKVRYTRGGKPLEKSITWDGCDPVGSTLGCIVEEHNLA
                     NLILGEERAEFGGIRNLRKPINLLEHGIDDVFFFDDEINGWQEISPDWLTDA"
     gene            28533..29234
                     /locus_tag="IT904_001430"
     CDS             28533..29234
                     /locus_tag="IT904_001430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191234.1"
                     /translation="MSISDGVKVLIITVCGYTFTYAYYLGYYAAKEIPVEFIDVSLLS
                     MMKIGFGTCAILSFALYFLDFFKEEIEDPVSVSVNTKRFLIRHSIGIITMLLFTVNMT
                     LSGKWLSLIPPFMIWYVCLKQDITISFKDSETKRFKFKVSINPLKKLGSTIVAEKLNV
                     RSYYACFSWYLALLFLLFNVGGVFSHMIKDTLVCNKDIHVVSVGDSDVLVTRDHKNFE
                     FMDKKDCKFSIPEIK"
     gene            30516..30950
                     /locus_tag="IT904_001431"
     CDS             30516..30950
                     /locus_tag="IT904_001431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191235.1"
                     /translation="MEIDDFRRSEGRMRDLVDDCDYIETRKKYYYQEAKKMLDNFRVD
                     INIIHADDEGCGKLTFEVYQDIKFQLTIKNRIKFIAVDLQLFETDNYSREYLSDEHRY
                     SFSIGLGDKFSDVDTPLESHNYKQHFRHILITLMKNIARIED"
BASE COUNT         8837 a         6807 c         7257 g         8519 t
ORIGIN      
        1 tatttttcca agtatctatg caatcctaca cagattctat acagtatcga catagtttcc
       61 ttttggtata acttattgaa atatataaag aatctgtgaa gccccttttc agggtaaact
      121 gtatcgtgtt agtcgtccct ctctcgaccc ctagaaaagt gctatataca gggctatata
      181 acgctatata gatagttggt cacatggtgt aactatcaaa ctacttaaca gtttcctgcg
      241 tgaaagttgg aaagtgatag ttatagttac cttgtcaaat cttcgtagtc cattcttaaa
      301 aaatcactta cagacacctt aatatctata cagatttcat gtagaacacg attagtgcgt
      361 agaagagtca ttatgttaaa aacgaactgt atacgtatca gtactgattt ataagggttt
      421 tgtgtatttg aagataagag gctatacagc accttgagag catgtcttct aagtcattga
      481 tacataaggt cacagaagtt tgtgtctgtt acagagaaaa ctggatagga cttcacagaa
      541 ctacaaaatg ttacagctat tgataggtga aatcttcttg atagatgcct tgaagtgtgg
      601 tgaaggtttc ttgaggcgga gctaaacaga tgctatgaag aaagtcaata gttggttata
      661 gatattttaa ctgcttttgc aaagggggtg ctatatcgtg aatctggggt acttaacagc
      721 catcaattga gtgtagatat tttttaagat tatcggtgaa gggggaatgc agagggggta
      781 ctacgatttt tataaaaatt ttcagaagcc aaataactac tgtgcatatt ttgcacaatc
      841 ctgccaaacc tcatagaaag ctctgtagcg agttttaacc ccatttttag gggtaacctg
      901 tagggatgta ccacccacct taaaataaag gggtaaaatc gcttagaatg cgttacagag
      961 cgtctgagaa gctatattca cagattttac taaagtctca gacttctaag tagtcttttt
     1021 aacgggaggg ttctttctca tgtatcaccc tctcttcccg ctaagtaagc tacacagacc
     1081 tagagaacat gccttcacag catctatatc aggtcagatt tagagggttt agagggtttt
     1141 gatagaatct tcaaaggtac ttgacagata actatgaagt tgttaggaag aattgtttaa
     1201 cgggaggaaa gatacgatat aacccgaagg gaaagtatca tcttaacagt gattgtaaga
     1261 gatacctgaa aagttcctct taacagaact gccaagaaga gagggaacga taagaccaga
     1321 ggtagtgacc tgttaaacag ttcgtttcaa agctcttaag agtctgttaa gtaagggcta
     1381 tgtgaacaat cttaccattt tgtcaatgct cttgtctttg acctaaatca ataaaatgta
     1441 tcgtttgata tggttttaag caagatggct aaaaatcttt ttagaggggc gcttcgcttg
     1501 tgtcactatg ttctttctct ccctgtctaa gaggggagtt acagaggtct ttcaagtctc
     1561 aatagacaaa ccgaggaaca caggaaaccg agggaagaaa aactattgtg attaccttga
     1621 acaaggtctg taaaggtcgt ttaagaggtc ggttaagaaa ggagtttccc tctctggggt
     1681 tgtcgctctt ctctcgctta gtggattgat taggggttgt gttatacaag cccaactaag
     1741 aagacctctg aagcttccct gtgaagaagc tgaacagcta ttatattggg tgattacaac
     1801 tcattgaaaa ataagaactt ttagaattta ctgttaaggc ttcagagtta actgtgaaga
     1861 gacttgtcag ttctctatat atggctaatt acaacttatt gaaaaataag aacttttaga
     1921 aattactgtc agagaggtga atgcttgata gatacagctt tccggttctg tcaagctatt
     1981 ttttaataga tagcacaaaa tgctaaagat acccctatac catcacgtac agggggtttt
     2041 gaagggagtt taacggggtt ctgtgtggtc attatcctta aggtcaagcg tccatgtacg
     2101 accgaacagt ttaaagacaa caagcactct gacgaggacg tagcgaagta gcagacctgc
     2161 aataagtcca atacctccac cagcccatac atcaggtgac cccaattctg gagggagatt
     2221 taccattaaa tttccttgta agacgctctc aatcgcattc tgacacaaaa aagcacctga
     2281 ccttatagtt gacctgaaga ggccatttaa ctcgttacag agcgattgaa gaggcctgtg
     2341 tgcaatgcaa taaccatgtg accactctca tagccacgcc tcatatttta ctctccccat
     2401 agaaccatta taccacagat gttaatgaaa tgttaaaaaa tgcttgacat tagtctttga
     2461 aaatgctagt attgtatata agttaacaga gaattaacaa ttataaggac tagcttatga
     2521 atgaacgaca gagaatttag agcgatgctt caggcatcaa gagaacgcaa caagcacaac
     2581 tcctacgctt acacaaacaa ccccaccagc tacgaactac cagccttttc caaatccgaa
     2641 cgtaaaagca ttgaagcggt cattcgtagc attacgccac gaggcagata tatgccaaca
     2701 cgcaaaacaa caaagaataa cttaaaaact ttcttgatga gttttgacag ctacgaacaa
     2761 ttaccgtcac gaattgaaga tgtaattatc ggcacttgtc gcagctttgg aagggataac
     2821 tatcaccgga aagttttcta tttgcttcga agccttgatg agattaactc aagcacagtg
     2881 acgagccatc ttcaaagaca agcaaccaga cttagccacg agctaccatc tgataagtat
     2941 tgcgctaatt taaccaccat ttgcaataaa gttatcgaga caatcaatca tcatgttggg
     3001 gttggtaata tcagccttac gaccagtgag ccagactttg aatttgacgt atacgcacaa
     3061 gcagaggaat tttaaaatgg gaaataaaca cacactaacc agagttacaa agacagcctt
     3121 tgaagaactt aaccgaatca aaaacattac aggtctgcca cactcagcag tgatgaagct
     3181 tgcaattaac cgtgatatta acgagcagga tttgcttaat agtcgtttag aggcagcaag
     3241 acaccatatc aggattaagc aagatgacct tgatagtctg aaatctttaa atgcttttgg
     3301 actggattta gcaaggttac taagtatcaa aattcacaaa ttagcgatgg aggtttaatg
     3361 tatgactttt ataaacatcg tgaccatttg gtaagatttg acggtaaagt ctggaaagac
     3421 gtaacacaaa ccaataagcc caagtttacc caccagcaat tagcctcaag atttgatatc
     3481 tgggctgaag cacaaaaaga taatggtgat ttcgagttta tcagaggttt tgattatgct
     3541 tacgaaagcg accttaaaga cgccattgaa ttaatggaga acttcaactc tattaaaaat
     3601 agtgacaagc acaaagccaa cggtaacaga tacgataaag atttaatcat tgatgcaatc
     3661 accaaaggac aatcacaaag ttatatttct gagaaatacg gcttatcacg tagtcaatac
     3721 tacaagatta aagcgtctgt gtaagtctgt gagaatgagt gagactcaaa gatgagctac
     3781 cctattacta aggtgtctca ctccctctgt aacccgcatg gttactggcc tcagaatggc
     3841 tattttggca gatttaagat gtgtttttat aggattttaa aaatgaataa tttcccacta
     3901 cagattttta ttgataccga caccgccatg atgattcagg ctttcactga tgtaggtgtt
     3961 agcattgact ttgataagtt gttagagtta atggctgata atgcggaaag catctctgac
     4021 tttattcact cggtggagtt taacgagcct cgaatgatgt taccaattac agacagtaat
     4081 atgaaaagat tggttattga gcaaacgaac aaatacagta tttccccaga gcaatttttg
     4141 aaaggtgctg tgacaatcct gtacagcgat aatattttgg ttacggattc tgtgagggtt
     4201 cactaatggg cttaataatt gcttggacgt tgcttggctt atttgttggt tgctgggtgg
     4261 gtaacacagt aaataagaat tgatgttatc aatctgttaa aatctgttga caaataacct
     4321 taagcatgag atgatggtaa taggggggag caactcttcc tgaattaaac tattaatcat
     4381 tctacatcaa tgatagttat tctgagttgg aaaggtgcaa tgcttttcgt catggcctat
     4441 ttagcccaca ctagataggc tatctcaaaa agtctttgtt tattcattga atcctttaca
     4501 gattgcccta atcatgagtt agggcgtttt ttaagagttt ttaaagaaat ttcttcacca
     4561 gccctttata gcgttttagt tatctagggc tttttcagct tttaaaaagc atacttggca
     4621 aacgctttta agtctcttct aagttcgtag gctgtcaggt gtgcttttta aaaactgaat
     4681 attacttaga agaggaaaaa acaatgacaa acgatttata tattaacggc agaaagattg
     4741 atagcttcgg ggactggtca ccacagggtg aacatccgct acatactatt cagcgtaaag
     4801 aacacgatga aagaattaag cttgagcaag agttaaagat gtcaccaaag gtaatttcct
     4861 ttgtgtctcc tgagcctcaa gaaatgcctg atgtatgcaa aactgaagca ctatttaatc
     4921 ttgagcgtga atattaccca ctgctaaaag cacagcgtat taaactagat gacgcttaca
     4981 gcaaagttac cctgatgcaa tcagctattg agccatcaga atttgagatt caggatgaat
     5041 tgtcacagaa accatttgtc tattacaaat atgaagacaa tgatggtttt ggtacttttc
     5101 ctgagaatat cccagacgtg attaataatc ttcctgatgg atatcgaatt gtgaagatgg
     5161 ttaaagcttc aagaggtgct ggtcagttta ttttcatgac cgataggagc gatgaagaac
     5221 tctgtgaaat tgctaaacag aacatcttag catcacgtaa caagcaactt gaaaatgtta
     5281 agttgtatct ctcaagagag cttcaggcta tgaaagagct tgttagtgaa tatgaatcac
     5341 agaagaagat tgctatgcag gctgatattg agcagttagt gaagatttct cagaaatatg
     5401 caaaggcact ttaactatga attaaaagga ggtggtgaat gattgattta acagaacgct
     5461 atgagcttat taaagcaatt agtgaacgat tgaaagaaca acccaacaaa ccaaaacgaa
     5521 tcaaatcatc cacctctaag caaaagaata gaatcagaag aatcgctaaa tggtgtcttg
     5581 ataaattacc aagtgattgt cagattgtcg gtgaagatgg taattattat ctggttaggt
     5641 aaaaagattg gctgggtaaa aatcaacggg agagattatc gagtgacatc ggtagctctc
     5701 ccttttttgt atatgagata gtcttctatc gaatcctgtg tgctacgtga tgattcggat
     5761 aagaattatc ctcatgaaat ctgtaaaact aatacccaca ctgaaaaata gaatttacgt
     5821 acaacacgct taaagaggtg aaacatgaat gagcaaattc acagttgata gttttatcgt
     5881 tgagctaggc ttcaatgaaa acgttattaa gggactgcaa agagttgaaa aggcagcttt
     5941 gcagtcagca caaagaattg aaaagaacct aaataaaggt ttcaggatta atacaaagca
     6001 gcttgatagt aatttaacgg cttctttagg gcagcttgaa aggaagttta ataaggcctt
     6061 tgacaagatt gaacagagag caagaaatac taaagctttc cagtttacca ccagatttaa
     6121 tgacactgtt aatcctccaa aacaaccaag acagccacga attagcggaa acagagcaat
     6181 cacagcagct tactcagcga atatgagcaa gctaaaaggc cttaacccaa ttgtcgagaa
     6241 atacattaag agtcagtttt acgggctatc tgctaaagcc gggtcaatgg attcttcaaa
     6301 attcaatgag aagttagctc agttaaacgc ttcagtcaga gaagcaattg ctaaagccag
     6361 aggtcataca tccaccagca ttaatggtga cagagcagct aatagtcttg ataacctgtc
     6421 ttcagcagca gttaaaacag caggtgcatt tatctcaatg caggctgtat tagccagtta
     6481 tcagaagatt atggaaattg gtttacagcg tgcaagtgct gaacgttcaa ttgatttcgt
     6541 atttgataaa gatgcaggtc aggtaaaaga gttcacaaag tcattagctc aaacaacggg
     6601 tttagatatc gctgaactgc aatcacagtt tgctggtttt ggtgcttcag ctaaagagtc
     6661 tatgggtatt caaggttctg aagaactttt taaaaatatg attggctatg ctcgtctgat
     6721 ggggagaagt gaagaagaga ttaagcgagc actgacagca ttatcacaga tggctggtaa
     6781 gggtcagatt atggctgaag agcttaaggg gcaactggct gaagctgttc ctggtatggt
     6841 gcaggtgttc gctaaagcaa ccggaaagag tgaacaggaa ttatttgatg ccatgaagaa
     6901 gggggcttta aagtcttccg atactcttca gaaggtcact caggaactga ataaacaaat
     6961 caccgctaaa ggtggctgga aagccatttc agaaagtact caggcacaac tagggaactt
     7021 gaaaaactct tggaatacca cgcttgatag tatcttcaga ggttctgaaa atggtctgca
     7081 agattttaca cgaagcctaa caacgctttt aaacgctctt ggtggaagtg gtaaaagtct
     7141 tggtgaatct cttggtagtc tcatgacttc tatgtcacat ggtgtcgata gtctgaccga
     7201 tattagttac aaggtgcgag cgttctttga tgaggtgaca ttagcttacc gttcactaaa
     7261 tgacactcag aggaaaattg ttgatggttt atctgatggg ttaattgatg gtttgaagat
     7321 gcttgctgtg gttgttactg ctcaaaaggc tgcaaatgta gctggaggct tgttaaacct
     7381 tgcaaatgct gttacttcat tcggtgatgc tgttaataaa gctaaagctg gcaaggctgg
     7441 ttcttcaggt ggtgcgctaa gtaaagtcgg tagagttgtc ctttatgctg agttggtaaa
     7501 ctttgctctt gataatttaa accctgaagc agctaagaat ccgaacttac accaatcagg
     7561 tattggtaca gaaacaaact tcaatcctga atacaaaggc gattctaaac caaaccaaat
     7621 taatgagggg tgggctaaga ttggtagttc tatcgctgac tggtttaatg gtgcagctaa
     7681 taaaattgct aacttacaag agccaatgag taaccttgtt aataacacac aatcaactcc
     7741 accagctatt acaggtgaca tggttaatag caatgctcag ttagctatgg ctgtgtcaga
     7801 gttaggaaga aaggttgatg ctttaacagc agaagctaag aagcccttgt cagttaatat
     7861 caaaggtgaa gcaattgtta ggccggatga gccatctttc tttactttca atcaatcagc
     7921 taatgaccag ttcacgcagg atatgctctt aagttcaagc tatccagagg aagaatgatt
     7981 tcttatatat ccgtgtgcta cgtgaaatct tctgaacaga tatacccacc aaaaattatt
     8041 ttttacgtag ctcacgctta acagagaaaa cacttatata tcaaaggctt ggttaaaaat
     8101 gattaaaaat acactaagcc tttgaaatca aaggagaatt taatgatttt tagcaatcaa
     8161 atccgctaca gtggtaaaga tggcatctat ttccacttaa gagataatgt agatgccttc
     8221 ttaaccttat cagcaactga aaacatggaa tttgatagtc ctatgcaggt gactacacag
     8281 aacatgcaat cagggcaaac cgtcacagat aatgtgcaaa gagcacccag aacaatcact
     8341 attagtggtg tcgttgtagt tggctatgaa ggaagcttat tattaactcg tcagggtcaa
     8401 ttagtagaga acttcattga cactctggaa aactggcgtg accagaagca gattatttct
     8461 gtcatctgta aagatggaat taaaattgat gattccatta ttacaagctt taaagcctct
     8521 aaagatgttg gtatttcaaa cggcctaaga atccagttaa cttttcagga aattaacttt
     8581 aaagctattg ttggtcagac tgatatttct gctgctactg gaaagacttc taccacgaat
     8641 gatggtggtg caacttctaa gaagaataca gggaacacta caacgaatct tggaagccct
     8701 atgctgacct gtaaagagct ttttagctat tcggcaagtg agttatcaga tgaagcgtta
     8761 aaagcccgtg taacgtgctc taagagcgtt agcgttaaaa atggtcaaag caccttcaca
     8821 gactcagcta attcaggtgc tgcaaaggta ctcaaggcgg gtaatgcttt acggaagttt
     8881 tctgtgaatc cgaacaagaa gggcacttat tgagacataa aaaggggctt catagcccct
     8941 gattgctaca gttgataagc gtctatttct agtagctctt tttcagcaat ttcaaagata
     9001 gatgttccat catttagagg tagcgggtct gtaggaacaa ttatttcagt gtcgttctgt
     9061 agcgttacag ttaccgaatc cttacccaat gtaagccctt ttagatgctt ggacaaaatc
     9121 aactgacact cgacacgacc cgctttagtc ataaggtcaa tatctttaag ctggggtagt
     9181 tccttatcag tgagtgaagc cttacgcctc atgttatcaa tcatggtctg tagttctgtg
     9241 cgcttctggt tcagttcctt aagctgaatt tttagttcat caatatcatc catctcaaga
     9301 agtagggcgg tagctttctt gattctggct gtgacaattt caacctcatg ctgtagagat
     9361 gcaatcttcc cctctgcttc tccatcattc cccacgtcaa tcatccccag aaaattatca
     9421 atcatccatc ggtcaaacgg tttacgcttc aacggttgaa catcacagcg accgtctagg
     9481 tggttattgc acctgtagac ccccgcatac ttattcttgg ttccggtagg atggacacta
     9541 gccccgcact gacacctaaa cagacctttg aagatgttaa tcgttagtgg gttatcactt
     9601 gcaggtgtac ggcctttacg atttttatcg aggatttctt gagctttatt gaacgttgaa
     9661 gcatctatag cggcttcgta gtagttcggt atagcggggc ggtcattgcg ttgtgatgct
     9721 ggtaacgttc ctattgttgt ctctgacctt aagatggtgc gtacagagtg aacactaaac
     9781 ccattagcat ctttacggcg actaaacgac tttataccgt tgctttgaag gtgacgggcg
     9841 atgttatgca gactttcacc acttagcagc agcttgaaaa tcagtcttac agtctcagcg
     9901 tgttcattga gtttaaaagt atcggggtca atccatccgg gaaccatctt tgttaaacgt
     9961 tgttcaccag caagggcaag ttttgcacgt tgctcccatt tctgagaaac acggaaagac
    10021 ttctgcttag attcctgatg cgctatgtag gctttgacac gctggatgat tgagattcct
    10081 aaatcatcgg ctaaagcatc ctttgtgtag ataagaccat cttgtagcgt gtgaatctct
    10141 aacccgttgc ctaagatgcg attcatcaag gcttctgttt caaggtgttc taagcggcct
    10201 atacggtcga tactctcaac aagcaaaatc gtaccttctg gaaactcccc agactcaata
    10261 cgggctaaca tgtcaccaaa ctgaccagta agatgcttac ctttgtaacc ggatagacct
    10321 tcatcactga aagacgctac tacttgagca tcgctattct tcttcagcca ctgcttgaac
    10381 aggtcgtttt gtcgtctggt tgaatctgca ccctcagcgc cttgtatggc tgaactgtac
    10441 cgaaggtatg taattacgct cctcatggtg ataaactccc atgcaattga ttctcattta
    10501 aagataagct tataaccgtt atggctaatg ctacaatgcc gaatagctac aatacaatga
    10561 cccttgattt acctcgccgc ttcccctggc cgacgttact ttcggtctgc attcatggtg
    10621 ctgttgtggc gggtctgctc tatacctcgg tacatcaggt tattgaacta cctgcgcctg
    10681 cgcagccgat ttctgtcacg atggttacgc ctgctgatct cgaaccgcca caagccgttc
    10741 agccgccacc ggagccggtg gtagagccag aaccggaacc tgagccgatc cccgaaccgc
    10801 caaaagaagc accggtggtc attgaaaaac cgaagccgaa acctaagcca aaaccgaagc
    10861 cggtgaaaaa ggtacaggag cagccaaaac gcgatgtcaa acccgtagag tcgcgtccgg
    10921 catcaccgtt tgaaaatacg gcaccggcac gcccgacatc aagtacagca acggctgcaa
    10981 ccagcaagcc ggttaccagt gtggcttcag gaccacgcgc attaagccgt aatcagccgc
    11041 agtatccggc acgagcacag gcattgcgca ttgaagggca ggttaaagtt aaatttgacg
    11101 tcacgccgga tggtcgcgtg gataacgtac aaatcctctc agccaagcct gcgaacatgt
    11161 ttgagcgtga ggtgaaaaat gcgatgcgca gatggcgtta tgagccgggt aagccaggca
    11221 gtgggattgt ggtgaatatc ctgtttaaaa ttaacggcac caccgaaatt cagtaagcat
    11281 aaagtcaaaa gcctccgacc ggaggctttt gactattact caacaggtaa ggcgcgaggt
    11341 tttccttcag gatcaaccgc gacatactta aataatgctt ctgtcgcttt ataccgttgc
    11401 ccgattggtt cagacgctac ttttttcacc cacacttcaa tattaatgct gaccgatgtc
    11461 gtccctttct ggacacagcg tgcatagcag cacaccacat cgccgaccgc aaccggccgt
    11521 aagaaagtca ttccttcaac ccgcacagtc actacgcgac cgtgggcaat ttcttttgcc
    11581 agaatagcgc cgccaatatc catttgtgac attaaccaac caccaaagat gtcaccattg
    11641 gcattggtat cggcgggcat ggctaaagta cgtaaaacaa gatcgccctg agggacgtta
    11701 tgtgttgtag acatggtaaa accgacttaa aaggaaaatc acaggcgcga tgctactatg
    11761 attttcctga ggagaacaga ggagatacag cgccagcccc gaaggactgg cagtctggtt
    11821 aggatttatc ttcctgcggc atgtggcggt agatatagat accgcttaac aatgtgaaga
    11881 ttaaggtaag ggcggtcagg ccaaagactt taaagttgac ccaaatattt tgcggcagcc
    11941 agaatgcgat gtagatgttt gccagaccgc aaaggataaa gaaaacagcc caggccagat
    12001 tcagcttcga ccataccggt tgcggcagcg tgagttcttt acccagcatc cgctgaatta
    12061 gcggcttttt catcacccat tggctgacta acagggctcc cgcaaataga gcataaatga
    12121 ctgtaacctt ccatttaata aactcatcat tgtggaagaa cagcgtcaag ccaccgaaga
    12181 cgaccaccag aacaaaagtg atcagggcca tcttctcaac cttacgaaag cgaacccagc
    12241 tatagataag cacaatcgcc gtggcgacga tcagcgccgc agtagctgca tagatgtcat
    12301 aaatcttgta aaacgcgaaa aagacaacca gcggtaaaaa atcaagaaac tgcttcattt
    12361 tacgattccg taatcaaatg cttaaggtta ttggcgaatc aacatataca ggcggaacag
    12421 atagatgagc aatatggctg aaatcaggtt gctcaaggtg ttcgccagta cggcaccaat
    12481 ttccggggtt aatgcggcaa aagaagaggc aaaaagcagc aacagtgttt ttgccagcaa
    12541 ccagctcagt actgcgggtg ccaccagacg catattcgcc caagtcagcc gcatactgct
    12601 acgcatcgag gcaaaaatgc ccattttgtc ctgtaccagc atcaccggtg ccagggccag
    12661 caaaatggcc atgataattc ccggaacgac caccagcatg atgccaatct gtactaaaag
    12721 ggtagtcaga aaaatcagaa taaataactt gggcaatatc ggcgcactgg caccaatagc
    12781 tcgcagcgca ctgactctct gacccgcaga caccagctgg ataattaata ttacgcctcc
    12841 ggcgagaatg gcgttaccga tcaatcctga aaaagtggaa gccgctgaag cttgcagcaa
    12901 aatttgttgc tgttccggtg acatattctg aaccaggttg aacaacccac tactgccgct
    12961 aacgggtacg ccgtcattga gctgcgcaag ctgtgcatca ctgggtgaga aaacatgccc
    13021 taacaccact gtgataaacg cacatagcaa cgataccaac agaatggtca taaattgatt
    13081 acggaagaaa tttccggtgt cacggtatac ggactgcgcc gtgatagaca tgcactctcc
    13141 ttgagtttta tgcaggtgtt aattagcggg caattgtacc ctggttaagc ctcgggtggc
    13201 agcatcaggt cttgtatgga aaagcatatc tttgtaaagc ggaggtaatt gctggccttc
    13261 aggcatgggc aaatcgcggc aagtggcgac cgagttaata tttgcgtagc gaaaatattt
    13321 aaaaattgat ttaaatcaca ttaaccagga ttctcaatgc aacttctaaa ttaatccaga
    13381 tcaataaagg gtgaattatc atatgtaatg tgatctatgt aggatcattt gttactccaa
    13441 tgtaggtata ttcgtcacgt ttttataacc ataacgacgg agcggatatg aaaaagttaa
    13501 cagtggcggc tttggcagta acaactcttc tctctggcag tgcctttgcg catgaagcag
    13561 gcgaattttt tatgcgtgca ggttctgcaa ccgtacgtcc aacagaaggt gctggtggta
    13621 cgttaggaag tctgggtgga ttcagcgtga ccaataacac gcaactgggc cttacgttta
    13681 cttatatggc gaccgacaac attggtgtgg aattactggc agcgacgccg ttccgccata
    13741 aaatcggcac ccgggcgacc ggcgatattg caaccgttca tcatctgcca ccaacactga
    13801 tggcgcagtg gtattttggt gatgccagca gcaaattccg tccttacgtt ggggcaggta
    13861 ttaactacac caccttcttt gataatggat ttaacgatca tggcaaagag gcagggcttt
    13921 ccgatctcag tctgaaagat tcctggggag ctgccgggca ggtgggggtt gattatctga
    13981 ttaaccgtga ctggttggtt aacatgtcag tgtggtacat ggatatcgat accaccgcca
    14041 attataagct gggcggtgca cagcaacacg atagcgtacg cctcgatccg tgggtgttta
    14101 tgttctcagc aggatatcgt ttttaattcc gcacaaaaac gaccccgtaa tatacggggt
    14161 caataaggac atggtataaa gggtattatt tcttcgcttc tacgccatca gtttcagagc
    14221 gaattaaaaa tttctcagtt gtttgcggaa tattctgaat cagccagtcg gccatttgct
    14281 tttcctcatt taaaatcgct tcgatggttg gaattgaagc tgtatcaccg gcattttttg
    14341 ctgctgctaa tagtgaggtg taacaggcga tttcaaattg ctcgaagaca tatccgctaa
    14401 tagagccttt gactatttca tcagaaggga atataccacc gattgactgc ccaagcgcag
    14461 ccattttact catggaatct ttaatgactg aacgtgaaat gtcattacga tcaagaatag
    14521 tttccagttg aacaatctgg tttttggttt cactaagatg ttgttcaata cgagcgcgta
    14581 gttcaggata attatctata cggctggcca tggattcaag catagattcg gcttgctttt
    14641 ccattgcgtg ggcgtcacgt aaccagtcat gataatgttc aatacgattc atgaaaatgt
    14701 cctctttata aaatgactaa aagttaaaat tcatatttca ggctttattt tcggctttct
    14761 tatttacgtt attaatggcc agatcagtca gtttgatgtc ggtggccttt tcttcttcca
    14821 gggtttcttt cagaagcttc gctgctttac ggtaacctaa ttgttcagcc agcgtcgcta
    14881 atgtcccgta actggcaatc tcataatgct cgactttctg tgctgcggca atcagtgcgg
    14941 catcacgcac ttcgtttttc tcggtacttt cgatgacctc attagcttct tcaataagac
    15001 cttccattgc cacacatttc atgcgcttaa ttttcaggtt cgattcggac tccacaactt
    15061 gatcaatacg ttcaatctgt ccatgagttt cctcgaggtg cgcatgaaaa gcctgactta
    15121 atttttcatt tgatgttgct cttgcgagtt ttgccagtgc ccgggttaat tgtttttctg
    15181 cgctgtaggt atctgaaagc aggtgaataa atacatcttc aatggtcttc atattcatat
    15241 ttttctccag tgaaatcact gcgagccata cggcccgcag atgcaattat ctttatcagc
    15301 tgatattaat aattaacggt gaatcaggat ttgcctgatt tattaccacc gctttgttga
    15361 ccgccttttt tacccgcttc agatgcgcgt tgcggatcat ttttaaaatt accgccgcta
    15421 tgctgaccgc ctttacggcc tgcgtcggat gccttctcac ggtcttcggc gaaatttcct
    15481 gaaccaccac gatgttcggc catgttattt ctcccgttgc gttgcattgt ttcattaata
    15541 tgagtgttgt gtgtcgacac tcattaaaat tagtcgctaa tgagaattag tcaaattaag
    15601 cgcaacgaga agatagaggg aaaatatatt ttgaggaaca ttctggatat attaacaatt
    15661 acctgaggaa taagtgactt agagaaaatt attgatttta ctggtgttat gttgcgggat
    15721 taatttgttt gattatatta ctgttgggcg gaaaaatgac gtaagttgac gttcgaccgg
    15781 ggtaagcgaa acggtaaaaa gataaatatt aaatgaattt aggatttttc cggcttcatt
    15841 aaagaaagtt aaaatgccgc cagcggaact ggcggctgtg ggattaactg cgcgtcgccg
    15901 ctttcatcgg ttgtacaaaa actttcagtg ccgccagcat tttctctggc tcattaatat
    15961 gttgctcgat gattttaaca atggccgaac cagaaatcgc gcccgcagct cctgcatcaa
    16021 tcgctgcttt tacctgatcc ggggcggaaa taccaaatcc ctgcaatgga ggtgcagcgt
    16081 tgtactcttt cagcttcgca accagatgat tgaggggtaa cgcggcgcgg ttttctgcgc
    16141 cggtcacgcc tgctcgtgac agcaaatagg tgtaaccacg accgtaagag gctatctggc
    16201 gcagcaggtc gtcatcggca tttggcgggc agatgaagat aggtgcgaca ttatgacgca
    16261 acgcggcctg gcggaagggc gcggactctt caactggcac atcggcaacc agcaccgaat
    16321 cgacgccgac tttttcgcac tgggcataaa actcatcaat gcctttgtta aacaccagat
    16381 tggcatacat caacaggcca atgggaatgg tcgggtgttt ctggcgaatc agtgccagca
    16441 tttcaaaaca ttgtgccgga gtcacacctg ccgcaaaggc gcgcagagtg gcgttttgaa
    16501 tcgtcgggcc atccgccagt gggtcggaga aggggatacc caactccagc gcgtcagcac
    16561 cggcttcaat tagcgtatcg ataattttca atgactgctc aatgcccgga tcaccgagcg
    16621 tgacgaaagg aacgaatgcg ccttctttgc gctccttcaa ctgggcaaac agagattcgt
    16681 agcgttccat cagatttccc ctcgtgcttt caaaatatcg tgaacggtga agatgtcttt
    16741 atcgccgcga ccggaaaggt taaccaccag tagctgctct ttatccgggt tttcgcgcat
    16801 cattttcaac gcatgggcca gggcgtggga ggattccagc gccgggatga tcccttcgtg
    16861 caggcacagc gttttgaagg cttcaagggc ttcatcatcg gtaatagaca cgtaatcagc
    16921 gcgtccagtg ctgttaagat acgcgtgttg tgggccgaca gacgggaaat ccagtccggc
    16981 ggagatggag taagattctt caatctgccc gtcttcggtt tgcatcatcg gcgctttcat
    17041 accgaaatag atacccacgc gaccatgttt tagcggtgcg ccgtgctcgc cagtttcgat
    17101 accgtgacca cctggctcca caccaatcag gccgacgttg gtttcattga tgaaatcagc
    17161 aaacatgccg atggcattcg aaccgccgcc aacacaggcg ataacggcat ccggcaggcg
    17221 accttctctt tccagaatct gcgctttggt ttcttcgcca atcatccgct gaaactcacg
    17281 cacaatggtc ggataaggat gcgggccagc tgcggtgccc agcatatagt gcgcggtttc
    17341 gtaactaccg gaccagtcgc gcagcgcctc gttacaggca tctttcagcg tcgcggaacc
    17401 gctatgcacc gggatcactt ccgcacccat taagcgcata cgaaaaacgt taggcgactg
    17461 gcgttcaacg tctttggcac ccatataaat acggcatttc aggccgagca gggcgctggc
    17521 aagggccgac gccacgccat gctgaccggc accggtttcg gcgatgattt cggttttacc
    17581 catccgcttc gccagcaacg cctgccccag cacctggtta gttttatgcg cgccgccgtg
    17641 cagcaaatct tcacgcttga gatacagcgt ggtgttcgtc ccggctgtaa tgttctggca
    17701 tttggtcagc gcggttggac gcccggcata gtttttcagc aggtcgttga actgagcctg
    17761 aaattcagga tctttttgcg cactgacaaa agcttcttcc agctggcgca gagcaggcat
    17821 caggatttgt ggcacgtaca tgccgccaaa ctcaccaaaa taggggttaa gtaatgttgt
    17881 cattgttcct ttccttaata tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg
    17941 tctttgatgc ccggttgcga ctctacagca gaattaaaat caagtccggc gcagccggtt
    18001 tgtgccgctt ccacgcagtt atctgcgcct aagccccccg ccagcagaac gttgccaagc
    18061 gattgaccat ttaatagtga ccagtcaaaa cgttgcccgc ttccaccctg gccgttgtct
    18121 aaaacatatt tatcaacgtg ctgaaactcg cgggcgggca gggtttcacc gacgcttaat
    18181 gctttccaga tggcaacatg tgctggcaga gcttcacgca gcgtatcgat atacagctgt
    18241 tcttcattac catgcagttg cactgccgcc agcgataaca ccttagcttt gtccaccaca
    18301 tcggcaatat cgtgattgcg gaacacgcca acatactgca acggtgccgc agccatcact
    18361 tcctgcgcct gttcaacgtt gacgcaacgc ggtgatgtcg caacaaaaat caacccaccg
    18421 taaatcgcgc ccgcgtcata agctgcttta gcatcttgcc cacgcatcag gccgcatact
    18481 ttattctcac ccagcaacac ccggcgcacg gcggcgtgca aatcgtcatg ggccatcaac
    18541 gccgaaccaa tcagaaaacc gttagcgaag tggcttaact cgcgcacctg agcgtaagta
    18601 ttgatgccgg attcgctgat tactgtcacg ttgtgcccca gtttcggcgc aagctcgcgg
    18661 gtacggttga gatcaatcga caaatcacgc agatcgcggt tgttgatgcc aacgaccttt
    18721 gctcccaatg caatggcgcg ctcctgttcc tcttcattac tgacttcggt cagcaccccc
    18781 atctccagac tgtgagcgac ggcggcaagc tggcgatatt ggtcgtcatc cagtactgaa
    18841 agcattaata agcaggcatc ggcctggtaa tagcgcgcca gatagatctg gtaagggtcg
    18901 ataatgaagt ctttacataa aatcggctgc ggggcgattt ggctgacgat ggggaggaaa
    18961 tcaaagctcc cctgaaaata tttctcatca gtcagcaccg aaattgccga agcgtaatgt
    19021 ttataaatgg cggcaatgcg tgctggatcg aaatcatcac ggatcacgcc ttttgacggc
    19081 gacgctttct tgcactccag aataaacgcc gtgcgcgcac cctgtagcgc atcataaaaa
    19141 tgtcgcgtgc tcggctgaac ctcattctga aaactggcca gcggttgctg ctgtttgcgg
    19201 gcttctaccc aaatcgcctt gtctgcgacg attttcgcta aaacggtttg catcatttac
    19261 cctcgtgccg ccagtgcggt gactctgtcg taagcggaac cactgcgcag tacctcaaga
    19321 acggtttgcg cattggcttg cagatcttca tggccatgca ggcgcattaa catggcgacg
    19381 ttcgcagcga cggctgcttc atgggcggcg tcgcctttac cttgtaacaa acgtgttaaa
    19441 atgtcacggt tttcttccgg tgttccgcct gccagttgct cctggtggta gggtgtcagg
    19501 ccaaagtctt ctgcggtgag ctgatagctt ttaatttcgc cgtcatgcag ttcggcaacg
    19561 attgtcggcg cgtgtaatga aacttcatcc atcccgccgc tgtgcaccac cgccgcgcgt
    19621 tgatacccca gcacgcgcaa ggtttcggca atcggcagca ccagttccgg actataaaca
    19681 ccaattaacg ccagcggcgg atgcgccggg ttaatcaatg gccccagcac attgaacagg
    19741 gtgcgggttt tcagttgctg gcgaaccggc atcgcgtggc ggaatccggt gtgatacttc
    19801 ggcgcaaaga ggaaacatac acctaactca tccagcgcct ggcgcgattt atcggcgttc
    19861 atatcaagat taataccgaa cgccgccagc agatcggacg aaccagattt actggagacg
    19921 ctacggttgc cgtgtttcgc cactttcagc ccacaggccg cggcgacaaa cgcactggcg
    19981 gtagaaatat tgatactgtt gctgccgtca ccgccagtac cgacgatatc agcaaacaga
    20041 taatccgggc gcgggaacgg cgctgcgttt tccagtagcg cggttgctgc cccggcgatc
    20101 tcgttcgggt gctcaccgcg aattttcatg ctcaccagcg ccgccgccag ttgttccggc
    20161 ttcagctcgc cacgcaccac cgctgaaaac agctggtggc tttcttgttg gctaagcgtc
    20221 tgcgcctgat acagtttttc cagaatcggt tgcagcgtgt tggctggctc tagtttctgc
    20281 tgcgcccagg ccagcgtttg ttccagcagg cgagcgccct gggtggtgag aatggattcc
    20341 ggatggaact ggaatccaca aacgcgatcc gcatcgtgac gtactgccat caccatgcca
    20401 ttaaaatggg cgttgatggt taaaccggcc ggaatgttac tgccaaccag cgagtgataa
    20461 cgcgccaccg gcagcgggtt tgttaatccg gcaaacatcg cctgaccgtc atgttcaatg
    20521 ctggaggctt taccgtggag aatttcgccc gcctgaccga catagccccc gtaagcttcg
    20581 acaatcgcct gatgtccgag gcaaatgcca ataatgggca gcttgccacg caagcgggtg
    20641 aggagttccg gcatacaacc ggcttcgctc ggcacaccgg ggccaggaga aagcatcagc
    20701 accggattgc tcatggtcgc caggcgttca attaaggttt gcgccggaat atggttgcgg
    20761 taaatcacca cgttatgccc attgctgcgc aactgatctg ccaggttgta cgtaaaagag
    20821 tcgatattat cgagcagcag aatgtcagcc atcagaaagt ctcctgtgca tgatgcgcgg
    20881 tggcaatagc gcgcagtaca gcgcgggctt tattacgggt ttcgtcggct tccgactgcg
    20941 gaacagaatc aaggactacg ccagcaccgg cttgcacggt ggcgatactg ttttccacca
    21001 gcgccgagcg gatcacaatg caggtgtcga gatcgccatg cgcggtgaaa taacctaccg
    21061 cgccgccgta gctgccgcgg cgacgacctt cagcctcggc aattaactgc atagcgcgta
    21121 ctttcggcgc accgcttaac gttcccatat tcatacaggc gcggtaagcg tgcagggcgt
    21181 cgagatcgtg gcgcagctca ccgaccacgc gggagaccag gtgcatcacg taagagtaac
    21241 ggtcaacttt ggtaagatcg gcgacgtagc ggctgccggg ggtgcaaatg cgtgccagat
    21301 cattacgggc gagatccacc agcatcagat gttcagaaag ctctttatga tcggtacgca
    21361 tttccagttc gatgcggctg tcgaggtctc tgtccagtga accatcggca cgacgaccgc
    21421 gtgggcgtgt cccggcaatc gggtagatct caatctggcg gctggtggcg tcatatttga
    21481 gcgaactttc cggcgacgcg ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt
    21541 acgggctggg attactcttt ttcagcacgt aataggccgc cagcggtgac gggcagggca
    21601 gagagaaacg gcgagatggc accacctgga aaatttctcc agcgcgaatc gctttttgca
    21661 acaaacgcac tacgccaccg aactcttcat cgctctggtt acattcacaa cgcatatgcg
    21721 gcacggaaac caccggcagc ggtggcgcgg tttcggtcag ttgctggcga agatcgttca
    21781 ggcgagcagt gagacgttgt ttttcttctt cattcggagc aaacaggctg gcctgaatgc
    21841 gggtgctttt tttctgatgg tcaatcacca tcagcgtttc agcgagataa aaacagaaat
    21901 cagggcagtt attttccgct gacagttgcg gtaaatcttc aaaccccgcc acaaggtcat
    21961 aagagaacag gccgccgaag aacatggctt ctcgttcttc cttcggtaca ttcaacagat
    22021 tctgcaataa gcggaaagcg tcaaaaaccg aaagggagca taagcgagcg tcttcatcca
    22081 gcagtggact aacagggggg aagcgcagca cacggcagtt tggtaattgt tcattttcca
    22141 cacccgcagg cagggcgtta tccagtagtg tcagcagggc ttcgccgttg ccggaaaggg
    22201 actggattgt gacagtgtca cctaaagctg taatgcgtag cgcactgtct accagcagca
    22261 ggctttttaa atcatctttg ctgtcgatat ctgcggattc cagcagcagc gttgccggac
    22321 gatcctcaca caattggtga aaaagcgcgg tgggattgtc gcgataagcg ccttcgcagg
    22381 ttagcagttc gagagtcggt ttttgtgttt gcattgttat tctctaattt tgttcaaaaa
    22441 aaagcccgct cattaggcgg gctgggtatc tgattgcttt acgcatggtg aatacactgc
    22501 ccgtttcagg aagtgcgcca ccaagctttc agtacgaaaa ttgctttcat tgtcgatacc
    22561 ctttttacgt gaacttgcgt actagttaac tagttcaatg attaattgtc aacagctcat
    22621 ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga
    22681 gtgcgccttg agcgacacga attacgcagt gatttacgac ctgcacagcc ataccacagc
    22741 ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgaga tgcgcgtcgg
    22801 caccctggcg atcaccgacc atgacaccac agctgcaatc gcgccagcaa gagaggaaat
    22861 ttcacgttcg ggactggcgc tgaatcttat tcccggcgtg gaaatttcca cggtctggga
    22921 aaatcatgaa attcatattg tcgggctgaa tattgatatt actcatccgc taatgtgtga
    22981 gttcctcgcg cagcagacag aacggcgcaa tcagcgggcg cagctgattg ccgaaagact
    23041 tgaaaaagcg caaatacctg gggcgctgga aggcgcacaa cgactggcgc agggcggcgc
    23101 agtgacgcgc ggtcattttg cgcgtttcct tgttgagtgc ggcaaagcca gttcaatggc
    23161 ggatgtcttt aaaaagtatc tggcgcgcgg gaaaaccgga tacgttccgc cacagtggtg
    23221 tacaatagaa caagctattg atgtcattca tcattctggc ggtaaggcgg tgctagctca
    23281 tcctgggcgg tacaatctta gtgctaaatg gctgaaaaga ttggttgcgc attttgccga
    23341 acaccacggt gacgcgatgg aagtcgcgca gtgccagcaa tcgcccaatg aacgtaccca
    23401 gctggcggcc cttgcgcgtc agcatcattt atgggcatca caaggatctg attttcatca
    23461 gccatgcccg tggatcgaac tgggtcgtaa actctggctg cccgcaggcg ttgaaggcgt
    23521 ctggcagcta tgggaacagc cgcagaacac cacagagagg gaattatgag ccagtttttt
    23581 tatattcatc ctgataaccc acagcaacgt ctgatcaacc aggcggtgga gatcgtgcgt
    23641 aaaggcgggg tgattgttta tccaactgat tccggctatg cgctcggctg taaaattgaa
    23701 gataaaaacg cgatggagcg tatttgtcgt attcgccagc tgccggacgg tcacaacttt
    23761 accctgatgt gtcgcgatct ttctgaactc tcgacctatt catttgtgga taacgttgcg
    23821 tttcgtttaa tgaaaaacaa cacgccgggc aactatacct tcatcctgaa ggggacgaag
    23881 gaagtgccac gccgtctgtt gcaggaaaaa cgcaaaacca tcggtatgcg tgtgccttct
    23941 aacccgattg cgcaggcgtt gcttgaagca ctgggcgaac cgatgctttc cacttcgcta
    24001 atgctgccag gcagcgaatt taccgaatcc gatcccgaag aaattaaaga ccgtctggaa
    24061 aaacaggtag atttgattat tcacggcggc tatctcgggc agaaaccgac aacggttatt
    24121 gatcttaccg atgatacgcc ggtcgtggtg cgtgaaggcg taggtgatgt gaagcctttc
    24181 ttataacaaa ttgcgttcca cggatggaag actatggcag ggaagtttcg ctgcattttg
    24241 ctgttgatag cagggctgtt tgtatcatct ctaagttatg cagaaaacac ggagatccct
    24301 tcttatgaag aagggatttc gctctttgat gttgaagcca ctctgcaacc ggatggggtg
    24361 ctcgacatca aagaaaatat tcattttcag gcgcgaaatc agcagattaa acacggattt
    24421 tatcgtgatt taccacgact ctggatgcag cctgatgggg acgctgcact gctgaactat
    24481 catattgttg gcgtcacccg tgatggtatt cctgaaccct ggcatcttga ctggcatatc
    24541 gggttaatga ttattgtcgt gggcgataaa caacgtttct tgcctcaagg cgactatcat
    24601 tatcaaattc attatcaggt taaaaatgct ttcctgcgtg agggagattc agatctgtta
    24661 atctggaacg tgactggtaa ccactggccg tttgaaatct ataagacccg attttcactc
    24721 aagttccctg atatcgcggg taatccattt agcgaaatcg atctctttac tggagaagag
    24781 ggcgacacat atcgaaatgg ccgcatcttt gaggacggaa gaattgaatc cagcgatccg
    24841 ttttatcgtg aagatttcac ggtactctac cgctggcctc acgctttact cagcaatgcc
    24901 ccggctccac aaacgacgaa tattttcagc catattcttt taccctccac gtcatcgttg
    24961 ttaatttggt ttccgtgtct attcctggtt tgtggatggt tatatctctg gaagcgcagg
    25021 ccgcaattta cgccggtaga tgtgattgaa accgatgtca ttccgccaga ttacacgccc
    25081 ggcatgttac gtctcgatgc gaagctggtt tacgacgata aaggtttctg tgccgatatc
    25141 gtaaatctga tcgtgaaggg aaaaattcat ctggaagatc agtatgataa gaaccagcaa
    25201 atcctgattt gtgttaatga aggcgcgacc agaaataatg aggtattact gcccgcagag
    25261 cagttattac tggaagcgtt atttcgtaaa ggcgataagg tcgttcttac ggggagacgc
    25321 aacagactct tacgcagggc atttttacgg atgcagaaat tttatctgcc gcgtaaaaag
    25381 tcttcgtttt accggcctga tacgtttttg caatggggtg gactggcaat attggcggtc
    25441 attctctacg gtaacctgag tcccgtaggt tgggcaggaa tgagtctggt aggcgatatg
    25501 tttattatga tctgctggat tattcctttt ttattttgtt cccttgagct tttatttgcc
    25561 cgcgatgatg acaagccttg cgttaatcgt gcaatcatca ctttgtattt accactgatt
    25621 tgttcaggcg tggccttcta ttctctctat atcaatgtcg gagatgtatt cttttactgg
    25681 tatatgccag cgggttattt tagcgctgtt tgcctgaccg gttatctcac tggcatgggg
    25741 tatatttttc tgccaaagtt tacccaaact gggcagcaac gttatgcccg cggtgaagct
    25801 atcgttaact atcttgcgcg taaagaggca gcaacacaca gtggacgtcg gcggaaaggg
    25861 gaaacacgga aactggatta cgcgttgcta ggttgggcta tatcggcaaa tttggggagg
    25921 gaatgggcgt tacgcattgc cccttcgctt tcttcggcga ttcgcgctcc agagattgcc
    25981 cgtaacggcg ttttattctc attacagacg cacctaagtt gcggggccaa taccagtttg
    26041 ttggggcgaa gttattccgg tggtggtgct ggcggcggcg cgggtggcgg aggcggtggt
    26101 ggctggtaat taagctgatg ttaatcgaaa caagctttaa ccataaagat agtgtagtat
    26161 gttgcgcctc aaagccaggc cgataaacgt cgagtcgttt acttaaggcc tgaagagttc
    26221 aaacagactt tactatcagt gagtcagatg taagttctga ctcactgttg cacaaaagca
    26281 tatcagacgc ctgggaaggc cataaatcta cataataaat caagtcgtta gcgttaactc
    26341 tatgaaatgt ataacaaaag ggtcaacgca atgggtaaca gagtggacaa caaagccccg
    26401 aaattcacta ccaaacgcgg caacacttac tacatcaatt ttcgtctccc tgacgggact
    26461 ttctttcgcc gtagtttagg cactgattca ctaagggcgg ttgaagtgac tatgagccgc
    26521 ctaagccctt acattcccct tgttcaaaat ggctcgctgt ctgtcgagca attcaaaatg
    26581 catatcgacg gtttcagaga agccacccag cgcgattttg atacctatct gttggattgg
    26641 ttgaggctag gcgttgatga ggttaagcgg atgcctgacc tagggcattt ccagcgtaaa
    26701 ttagattctg acactgctat ctcacctgct gctacagtca cagaggcaaa aggccgtgcc
    26761 gatatacatt tgaatcgggt ttatagcggg gacgattcta ccgctcgaat gttacttgct
    26821 actttgcacc agaagaaagt ttctttcggt caacgagatt tagaccaggt ttatgaggtg
    26881 gcggggcaga ttgatatgca tcaggccatg ctacaccaag cttatgaggc gttttatgct
    26941 ggtgacattg tccgttatcg tgcccttgtt gactccatgc aatcacaatt ggttgaaact
    27001 gagagacgta aaaaaccagc tactaaacag gctgtagcaa ctgttgacgc tcctgaagct
    27061 gacaatacgc cgttactatc ggaagcctgg aaactgtttg tagcggagaa ggggaaaaac
    27121 tgggctatca ccatagctcg ggagaataat cgcttctacg aggtgctgat gcatgttctg
    27181 ggggatgttc ccgtaggtag cattaccaaa cagcatatac gccaaacatt ggcagtgatt
    27241 gagaaccttc cacggcgtaa tctcaaacca tattcagaga tgagcctctc ggagtgcata
    27301 gattgtgatg tgccagagga cgagctgatc agtagtgcca atgttaaaaa gcatctcaag
    27361 atttattcct catttttcaa agtgttcctt aaagatgaga aggatatctt agaaaaatcc
    27421 cctactgagg ggatcaagta tgaggtgcag gaaaacaagg gcggtcatta ctcaaaacca
    27481 gaaatgaaac gcttaataga acggttatca acttatactg attggcgtaa agattacttc
    27541 ctgaccttga tctacacagg tgctcgccgg ggtgaactgg cggctatccg taaggagcat
    27601 atacgcaagg atgaggaaac agggcgttat tacatattta ttgagggagg taaaactgag
    27661 catgcgcagc ggcagattcc ccttagtaaa gagatagaaa cgctgctgtt agcgcgtatc
    27721 aagcccctta agtcagatga tcttgtgttt ggagacctgc caacttacga gcatattgct
    27781 ctcgtgtggc gtagcctaat ggatgattgc aacgttccga aatataacga atttggacag
    27841 aaacgtgtga ttcatgcctt gcgccacact ttcatatctg aggctatagc taaaactggc
    27901 aatgcagcac tggtgcagtt tgtagtaggg catagtcgta ctcagtcttt ggggatcacg
    27961 gcaagataca cgcatagacc agcgctgagc caactgctaa cagtggtgga ttgcattagc
    28021 tagtgaaagg gttactctga tatttgatta tggacatttg atgagtagag gaaggtgaaa
    28081 atgacagcta gtaaagaatt aaaagttcgt tacacccgag gcggtaaacc gctcgaaaaa
    28141 tcaattacat gggatggctg tgatcctgtt ggaagtacat taggttgtat agttgaagaa
    28201 cataatctgg cgaatttaat cttaggtgag gaaagggctg aattcggtgg gattagaaac
    28261 cttcgtaaac caataaatct tctcgaacac ggtattgatg atgtgttctt ctttgacgat
    28321 gaaataaacg gctggcaaga aatctcccct gattggctta cggatgctta attaggtggc
    28381 tgctgagaga aaagaataat aaagaacaaa accagcaact gaatctggag agtaagttgg
    28441 gttctccaga ttcatttgtt agttgattat atcagatgtt gttttttata aaattttcat
    28501 ggtaaatgta acgtacaaag gaagggagtc aaatgagtat atctgatgga gtaaaggttc
    28561 ttattataac agtgtgtggc tataccttca catatgctta ttatttggga tattatgccg
    28621 ctaaagaaat tcctgtcgag tttattgatg tttctttatt aagcatgatg aaaattggtt
    28681 ttggtacatg tgctatatta tcttttgcac tatattttct tgattttttt aaagaggaaa
    28741 tagaagatcc tgttagtgtc agtgtaaata caaaaagatt tctaattcga cactcgatag
    28801 gcattattac aatgttattg tttactgtta atatgacatt gtcaggaaag tggctttctc
    28861 ttatcccacc atttatgatc tggtatgttt gtttaaagca ggatattacc atttcgttta
    28921 aagattcaga gacgaaacgc tttaaattta aagtttccat aaatccattg aaaaagttag
    28981 gtagtactat agttgcagaa aaacttaatg tccgttcata ttatgcatgc tttagttggt
    29041 atttggcttt gttattttta ttgtttaatg ttggcggtgt tttttctcat atgataaaag
    29101 atacacttgt ttgcaataag gatattcatg ttgtcagtgt aggtgattct gatgtactcg
    29161 taacaagaga tcataaaaat tttgaattca tggataagaa agattgtaag ttttcaattc
    29221 cagaaattaa ataaaaacag cgaagattat ttctcattct cgtctgtatt aaagatttgg
    29281 catagagaca agagccttta gtgatgtacg tgaatgtgta attactgttc tcatagatgc
    29341 ataacgtgtt cctcctgtgg attggtcatc gtaatctgtg cgccatttgc tagggttggc
    29401 agcagtgcca gcattaccca tggtaaggcc aataaatttt ctattcatcc ttgaacctta
    29461 gcatttttcc aatgatacgt tggctgaaga aaaacattat tcctccgccg atgacggcga
    29521 gaataaaccg ttaatccttg tccccctcaa aataatccag cccacgatct aacaaaaatg
    29581 tagtgcccca aaatgtaata agtggaatca caaacaacaa gatcattttc accgcaacta
    29641 gcggcttttt gatagccttt atgattaaac cgataccaag caccatgatt atgattgaaa
    29701 ggaatgcata tccgaagctg tttaatggat acattctaac cccgcccctg tgggtgtccc
    29761 gtctctgttc gttccagttt acatctttat gctgaaaata tccctagctc tacattctca
    29821 cgctcagtaa aaattacatg attttataaa gaattaacca ccaattaacc cctatttaaa
    29881 tcgattaaaa ttggctgttt ttgctctaca tccgagcttc taagcaagct aactcgatga
    29941 ttgattacca cgctaataat ttcaaaatat catgatgccg catgataact acatgcataa
    30001 gaactgaata caacatgcat ataacaggtc aaacgggtgt ttggtaactg ttatatataa
    30061 acaaacaatg taacctattg tatttattat gtttttgttc ttgatgtgaa ctacacgcca
    30121 aggtaggcga ctaagaaact cacctgatca ccacgcaata actctctcat ttcacgcaaa
    30181 tctaccgaca tcgcgtcaag gcaattcctt cattgatcac cactcaatca agaataattc
    30241 tcatcaaaag tgctgatcat ttatacagta aaaaatttat tcaaaaatga atatttttgg
    30301 aaatgttata ttataacaat gagagaagag ataatgttgt ttagcaatcc tgaccgttaa
    30361 ctaaagcaag cgttatttgg tttacggtaa tgtacatgtc ataagttgtt ttgctccacc
    30421 tgtacttatg atcacatttc aataaagttt cattttactt ttcatagtta aaagttaact
    30481 ttcattgtga actacacacg acacaagagg ctataatgga aattgatgat tttagacgta
    30541 gcgaaggccg catgcgtgat cttgtcgatg actgtgacta catcgaaact cgtaaaaagt
    30601 attattatca agaagctaag aaaatgcttg ataattttag ggttgatata aatatcatac
    30661 atgcagatga tgaaggctgc ggcaaactaa cattcgaggt ataccaagat ataaaatttc
    30721 aattgaccat taaaaataga attaaattta ttgcagttga tttgcaattg tttgagacag
    30781 acaattactc tcgagaatat ttaagtgatg aacatcgtta ttccttctca attggccttg
    30841 gtgataaatt tagtgatgtt gatacgcctc tggagagtca caattacaaa caacatttta
    30901 ggcatatact catcacgtta atgaagaata tcgccagaat agaagattag gattgaaatt
    30961 gtataaatag ggtaatagat ttttattacc ctgttcgtat aacctgctta tcacaccata
    31021 gggagttatt ccccgttcca tagggtgtat taacactgat acaggtacct gtatcaccat
    31081 cgatacaggg ggtgtattaa cagtaataca gggggtgtat taacgctgat acacaataag
    31141 aataattata agattaacta taagaatatt tataagatca tatcttttta tctttaatta
    31201 tagttaataa gttagtatct tcccgctatc gcgtggatag cattagtccc cgaggaaata
    31261 cttctgactg atgtcgcggg gactatgatt tgagtcttca cagtattcca ttacattcca
    31321 taccgtgatg cagcctcagg tgtaagatgc ttaaccacat taacgataaa cctgttaaca
    31381 tggtaaacca tctaacactc atatacagaa actaatcaat
//
LOCUS       DACAPB010000035        30577 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.035, whole genome shotgun sequence.
ACCESSION   DACAPB010000035 DACAPB010000000
VERSION     DACAPB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.035"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..376
                     /locus_tag="IT904_001432"
     CDS             <1..376
                     /locus_tag="IT904_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3191236.1"
                     /translation="FTIPLVNCPMTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAK
                     VGSFAGIAATGVGIRIDDAESGNIMPLNAMGNDNTVYQIPAESNGIVNVDLIAYYVST
                     VVPSEITPGEADAIVNVTLDYR"
     gene            456..1151
                     /locus_tag="IT904_001433"
     CDS             456..1151
                     /locus_tag="IT904_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3191237.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1180..3696
                     /locus_tag="IT904_001434"
     CDS             1180..3696
                     /locus_tag="IT904_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP3191238.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQQLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVSAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGVGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3707..4798
                     /locus_tag="IT904_001435"
     CDS             3707..4798
                     /locus_tag="IT904_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3191239.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(4841..5701)
                     /gene="rsmI"
                     /locus_tag="IT904_001436"
     CDS             complement(4841..5701)
                     /gene="rsmI"
                     /locus_tag="IT904_001436"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP3191240.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5766..7802
                     /locus_tag="IT904_001437"
     CDS             5766..7802
                     /locus_tag="IT904_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAP3191241.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRNLSWLQYQQGQVVPVS"
     gene            7760..8155
                     /locus_tag="IT904_001438"
     CDS             7760..8155
                     /locus_tag="IT904_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAP3191242.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8175..8765
                     /gene="diaA"
                     /locus_tag="IT904_001439"
     CDS             8175..8765
                     /gene="diaA"
                     /locus_tag="IT904_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAP3191243.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8775..9350
                     /gene="yraP"
                     /locus_tag="IT904_001440"
     CDS             8775..9350
                     /gene="yraP"
                     /locus_tag="IT904_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAP3191244.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9464..10504)
                     /locus_tag="IT904_001441"
     CDS             complement(9464..10504)
                     /locus_tag="IT904_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP3191245.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10577..11212)
                     /locus_tag="IT904_001442"
     CDS             complement(10577..11212)
                     /locus_tag="IT904_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP3191246.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11340..11858
                     /gene="yhbO"
                     /locus_tag="IT904_001443"
     CDS             11340..11858
                     /gene="yhbO"
                     /locus_tag="IT904_001443"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAP3191247.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11838..12281)
                     /locus_tag="IT904_001444"
     CDS             complement(11838..12281)
                     /locus_tag="IT904_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAP3191248.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12332..12634
                     /gene="yhbQ"
                     /locus_tag="IT904_001445"
     CDS             12332..12634
                     /gene="yhbQ"
                     /locus_tag="IT904_001445"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAP3191249.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12621..13124)
                     /locus_tag="IT904_001446"
     CDS             complement(12621..13124)
                     /locus_tag="IT904_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP3191250.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13118..13642)
                     /locus_tag="IT904_001447"
     CDS             complement(13118..13642)
                     /locus_tag="IT904_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAP3191251.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13851..14846
                     /locus_tag="IT904_001448"
     CDS             13851..14846
                     /locus_tag="IT904_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP3191252.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14855..15733
                     /locus_tag="IT904_001449"
     CDS             14855..15733
                     /locus_tag="IT904_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP3191253.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15814..16821
                     /locus_tag="IT904_001450"
     CDS             15814..16821
                     /locus_tag="IT904_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAP3191254.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16939..18183)
                     /gene="mtr"
                     /locus_tag="IT904_001451"
     CDS             complement(16939..18183)
                     /gene="mtr"
                     /locus_tag="IT904_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAP3191255.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18337..20226)
                     /locus_tag="IT904_001452"
     CDS             complement(18337..20226)
                     /locus_tag="IT904_001452"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAP3191256.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20219..20299)
                     /gene="yrbN"
                     /locus_tag="IT904_001453"
     CDS             complement(20219..20299)
                     /gene="yrbN"
                     /locus_tag="IT904_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAP3191257.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20406..21290)
                     /gene="nlpI"
                     /locus_tag="IT904_001454"
     CDS             complement(20406..21290)
                     /gene="nlpI"
                     /locus_tag="IT904_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAP3191258.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21399..23534)
                     /gene="pnp"
                     /locus_tag="IT904_001455"
     CDS             complement(21399..23534)
                     /gene="pnp"
                     /locus_tag="IT904_001455"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP3191259.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23781..24050)
                     /gene="rpsO"
                     /locus_tag="IT904_001456"
     CDS             complement(23781..24050)
                     /gene="rpsO"
                     /locus_tag="IT904_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP3191260.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24199..25143)
                     /gene="truB"
                     /locus_tag="IT904_001457"
     CDS             complement(24199..25143)
                     /gene="truB"
                     /locus_tag="IT904_001457"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP3191261.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25143..25544)
                     /gene="rbfA"
                     /locus_tag="IT904_001458"
     CDS             complement(25143..25544)
                     /gene="rbfA"
                     /locus_tag="IT904_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP3191262.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25708..28380)
                     /gene="infB"
                     /locus_tag="IT904_001459"
     CDS             complement(25708..28380)
                     /gene="infB"
                     /locus_tag="IT904_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP3191263.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28405..29892)
                     /gene="nusA"
                     /locus_tag="IT904_001460"
     CDS             complement(28405..29892)
                     /gene="nusA"
                     /locus_tag="IT904_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP3191264.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29920..30372)
                     /gene="rimP"
                     /locus_tag="IT904_001461"
     CDS             complement(29920..30372)
                     /gene="rimP"
                     /locus_tag="IT904_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP3191265.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         7450 a         8343 c         7608 g         7176 t
ORIGIN      
        1 attcactatt cctttagtta actgcccaat gactgggtgg gagcacgata acggcaacgt
       61 tgaagcgtct ttccgtctat ggctggaaac gcgagataat ggcaccgtcc ctaacttccc
      121 taatctggca aaagtaggct cttttgctgg catagcggca acaggtgtgg gtatccgtat
      181 tgacgatgcg gaaagcggaa acattatgcc actgaatgct atgggcaatg ataacacggt
      241 ttatcagatc ccggcggaat ccaatggtat tgtcaatgtt gacctcatcg cttactacgt
      301 atcaaccgta gttccatcag aaatcacccc aggggaagca gacgctatcg ttaacgtaac
      361 gctggattac cgttaatacg ttagggcgtt atctgacctg tcagataacg cccttttcct
      421 tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga
      481 tattaacctt gttgtgtagc ttctgtattg gccaggcgct tgcaggagga atcgttttac
      541 agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca
      601 aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat ggtaaaagcc
      661 gtgccccatt tattataacc ccaccgctat ttcgtcttga ggctggcgat gactcatcac
      721 tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg ttctacatta
      781 atgttcgtgc cattccagca aagaaaaaat cagatgatgt taatgctaac gagttgacgc
      841 tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacctgaag ggacgggtaa
      901 acgatgcgtg gaaatcactg gaatttaaac gtagtgacca ttcactcaat atatataacc
      961 caactgaata ttacgtcgta tttgccggac tggcagtcga taaaaccgat ctcacaagca
     1021 aaattgaata tatcgcgccc ggagaacata aacagttacc acttcctgca tctggcggaa
     1081 agaacgtgaa atgggctgcg atcaatgatt atggcggcag ttccgggaca gaaactcgtc
     1141 cactgcaata aaaaatataa aaacacaggt catcagggaa tgccacaacg acaccaccag
     1201 ggacataaac gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg
     1261 ctcactggca gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc
     1321 attatgctgg aaaccacaaa aagtggtatg caaacaaccg atctgtcgcg tttttcaaaa
     1381 aaatatgcac aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt
     1441 tcacagaaaa aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg
     1501 gtagaacaac tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa
     1561 gatgacgata gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc tgctgaattt
     1621 gatttcaatc atcagcaact taatttgagc attccccaaa ttgcactgta ccgtgatgca
     1681 agaggttacg tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac
     1741 tcgtttacag gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg
     1801 aatatgcaaa atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca
     1861 cgcaacgatc agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag
     1921 gcgttgaagt ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc
     1981 tacaccttta ctggcgtgca actcgcttcc gacgataata tgttgccaaa cagccagcgc
     2041 ggatttgccc caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa
     2101 aatggttatg tgatctatca aagcaacgtg tcagcgggtg cctttgaaat taacgatctc
     2161 tacccctctt ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacacaa
     2221 cgtcgcttta tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaag
     2281 tatagcgcga ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa ggaacccgaa
     2341 tttgctgaag ccacggcaat atatggtttg aataatactt ttacgctgta tggcggcctg
     2401 ctcggttctg aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg
     2461 ggcgcgttgt cgatggatat caacagagct gacacccaat tcgataacca gcactctttt
     2521 catggctatc aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc
     2581 gctgtcagct actatcgcta taccaacgat ggctatttta gttttaatga agccaatacc
     2641 cgcaattggg actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca
     2701 atatttgatg gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac
     2761 gataaaaaca ggaatatctc tgttggggtt tccggccagc aatggggagt tggttacagc
     2821 ctgaattatc aatacagccg ctacactgat caaaataatg accgcgcact ctctttgaat
     2881 ctcagtattc cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc
     2941 cagaaagatc gcccaaccca acatgaaatg cgtcttgatg gctcactgct ggatgatggt
     3001 cgcctgagct atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg
     3061 aacgccagtt accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac
     3121 agcagccaat acaattacgg cgttaccggc ggcgtggtta tccatcctca tggcgtgacg
     3181 ctctcgcaat atctgggcaa cgcttttgcg cttatcgatg ctaacggagc atctggcgtg
     3241 aggatacaaa actatccggg gattgctacc gatccttttg gctatgcagt ggttccttat
     3301 ctcacaactt atcaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc
     3361 gatcttgaac aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt
     3421 ttcaacgcca atatcggtta tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg
     3481 ttgccctttg gcgctcttgc cagcaacgat gatacggggc aacaaagtat cgtcgatgag
     3541 ggcggcatac tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg
     3601 ggaaatcagg cagatcaaca atgtcagttt gctttcagta caccggattc agaacctaca
     3661 acctctgtat tacaaggcac agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc
     3721 tcttataaca ggtcttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg
     3781 tggagccgac agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt
     3841 gaaccagaca gataacgtga caggacggga gtttacctct gcaacgctaa gtagcactaa
     3901 ctggcaatac gcctgttcct gctctgcggg taaggcagtt aaacttgtct atatggtcag
     3961 ccccgtactt accaccactg gacatcagac aggatattac aaactcaatg acagcctgga
     4021 tattaaaacc acattacagg caaacgacat tccaggactc acaaccgacc aggttgtctc
     4081 tgttaacacc cgattcacac agataaaaaa caacacggta tattctgctg caacccaaac
     4141 gggtgtttgc cagggtgata cgtctcgtta tggacccgtt aatattggtg cgaacaccac
     4201 ctttaccctg tatgtcacca agccatttct cggctcgatg accattccga aaacggatat
     4261 tgccgtcatt aaaggcgcgt gggtcgatgg aatgggaagc ccgtctacag gtgacttcca
     4321 tgatttagtc aagttatcga ttcagggaaa tctcaccgcc ccacagtcgt gcaaaattaa
     4381 tcagggcgat gttattaagg ttaattttgg attcatcaat ggtcagaagt ttaccacccg
     4441 caatgccatg ccagacggtt ttactccagt agactttgat atcacttatg actgtggtga
     4501 tacttcaaag attaaaaact cgttgcaaat gcgcatcgac ggtacaactg gggtagtaga
     4561 ccagtacaac ctggtcgcca ggcgaagaag ttcagacaat gtgcccgatg tcggtattcg
     4621 tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta tccttcccgt
     4681 tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg
     4741 tgagctggaa acaggaaaat ttcagggcac agcaaccatt accgtcatcg tgcggtaaaa
     4801 gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc tgctccagcg
     4861 catacttata cagcgcattt ttcttcacgc cgtgaatttc tgcggccagc gccgccgctt
     4921 ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt
     4981 cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt
     5041 catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg gtttcccagg
     5101 ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc gcaacgatat
     5161 cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg cgcggctccg
     5221 cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa aagccttcgt
     5281 aacagaaacg gtcagagggt aaacccgctg cgcttaacgc agtgatagca gcacacggcc
     5341 cgggtagcgg caccacgcgg atccccgctt cacggcaggt acgcaccaga tggtagccag
     5401 gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca
     5461 gcttcgccag cagcgtttca gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc
     5521 gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa
     5581 tcagatcaac ggcctgtaat acctctaacg cacgctgggt gatatccgcc agattgccga
     5641 ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg tgttgtttca
     5701 ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat atcactggat
     5761 acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc
     5821 tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata
     5881 tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg
     5941 atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa
     6001 ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat gctcagcgtc
     6061 gcgagaaaac actgctggcg gtagagatta aactggcgca gaaagatttt gctggcgcgc
     6121 aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa gcgcgttact
     6181 ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa
     6241 ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat gccacctggc
     6301 aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa
     6361 atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt aacgatcccg
     6421 acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat ccgggcgcga
     6481 aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa
     6541 tcgccctgct gttgccactg aatggccagg cagcggtatt tggtcgcact attcagcaag
     6601 gctttgaagc ggcgaaaaat atcggcactc agccagtggc agctcaggta gctgccgcac
     6661 ctgccgcaga cgtagctgaa caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg
     6721 cacaagcctc ggttagcgat ctgaccggtg aacagcctgc agcccagccg gtgcctgtaa
     6781 gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc gcagagctga
     6841 aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg
     6901 gcgcgagtat tgtggtcggt ccgttgctga aaaataacgt tgaagagttg ctgaagagca
     6961 acactccgct gaacgtactg gcactgaacc agccggagaa tatcgaaaat cgcgtcaata
     7021 tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg
     7081 accagggtaa acaagcgccg ctggtgctga tcccacgcag ttcattgggc gatcgcgtag
     7141 ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg caacaaaaat
     7201 ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg
     7261 gtagcccgat tactctcaga gcgacaaccg actccggcat gacgaccaac aatccaacgc
     7321 tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt
     7381 acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg
     7441 gtagccagag cggtgcaacg ctgtacgcca gctcccgcag tgcgcaaggg accgctggcc
     7501 cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg
     7561 gtaatctacc gttaatgcag caggcactca gcgcggtgaa taacgattat tcactggctc
     7621 gcatgtatgc gatgggcgtc gatgcctggt cgctggcaaa tcatttctca caaatgcgcc
     7681 aggttcaggg ttttgaaatc aacggtaata ccggaagcct gacggctaac ccggattgcg
     7741 tgattaacag gaacttatca tggctacagt accaacaagg tcaggtagtc cccgtcagtt
     7801 aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa
     7861 aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat
     7921 gcgtgaaggc cggaccacca tttttgtcga ggtacgctat cgccgctctg cgctttatgg
     7981 cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt
     8041 gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc
     8101 cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag
     8161 gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc
     8221 aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg acgctggttc
     8281 agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg
     8341 cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg
     8401 ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg
     8461 atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca
     8521 tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc gttacgcgtg
     8581 atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt ttgttagggc
     8641 cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag gaaatgcata
     8701 tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg
     8761 attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc gcgctgctgt
     8821 tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa
     8881 ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtgaaca
     8941 gcgcattgtc gaaagacgaa cagattaaga aagaagcgcg cattaatgta acggcctatc
     9001 agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct cgcgccaaac
     9061 agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc
     9121 cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg cgttcgcagc
     9181 tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag
     9241 tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat attgccagcc
     9301 gggtgagcgg cgtgaagcgg gtaactacgg cgtttacgtt tattaaatag cagccccttg
     9361 taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgataca aaccgtaggt
     9421 cagataaggc gtttacgccg catccgacat gttttccctc aaatcagaac aacagcgcca
     9481 gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat aaggctttcg
     9541 ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg agcgtcatca
     9601 acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt acaatcggaa
     9661 tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc accacccaca
     9721 tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca
     9781 acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc
     9841 tgccccagcg gctaaaaaac ccgccctgtg cttccggtat gtcaatttcg accggtgcct
     9901 gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca
     9961 ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaag cccataaaca
    10021 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
    10081 acacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc
    10141 ccggcaacga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
    10201 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
    10261 ccgccgcttt ccataccgcg aggaaataga tcatcgcgta atccaacgcc gcctgccatg
    10321 ggttagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa
    10381 acgctttgcc gtagtaaggt tcccatttca cataccagag accggcaatg acaacgagaa
    10441 agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag
    10501 tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
    10561 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
    10621 cgccttcatg ctcaggccgc atcgactctg ccagcatgac ccgcgccacg tctctcgcat
    10681 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
    10741 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
    10801 agttctgggc gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
    10861 agtgggcatt ggcacccatc gcactgacta ccaacatatg ctgcgcgccc agtcgccgcc
    10921 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
    10981 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct
    11041 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
    11101 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcgttaatc aacatccgca
    11161 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
    11221 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccaggcttaa tagaccatga
    11281 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
    11341 tgagtaagaa aattgccgtt ttaatcactg atgaatttga ggattcagaa tttacttcac
    11401 ccgcagacga gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta
    11461 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
    11521 tgacgcctgc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
    11581 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagtggc aaaccggtgt
    11641 ttgccatctg tcacggcccg cagttgctga tcagcgccga tgtgattcgg gggcgcaaac
    11701 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
    11761 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
    11821 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
    11881 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
    11941 ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg
    12001 ctttcttcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
    12061 accgttttcg gctgaccgtt taccgttccg gcaactgccg cctgcggccc gctcatctgc
    12121 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga
    12181 taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt
    12241 tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct cttttatact
    12301 gtgggcctga ccttaacatg cgatgcagac gatgacaccc tggtttcttt acttgatccg
    12361 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
    12421 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
    12481 ttctgctcca gtgggcgatc gttcgctggc gttacgggcg gaatatcgcg ttaagcaact
    12541 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag agctgttaag
    12601 cagtctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccaggccg
    12661 gtaacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
    12721 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
    12781 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
    12841 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
    12901 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
    12961 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
    13021 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
    13081 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
    13141 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
    13201 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
    13261 catacagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
    13321 ccggatcctg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca
    13381 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
    13441 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
    13501 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
    13561 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
    13621 gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
    13681 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
    13741 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
    13801 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
    13861 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
    13921 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
    13981 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
    14041 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
    14101 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg
    14161 ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag
    14221 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
    14281 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtctttg
    14341 ctttcggcag cctgtgcatt atgtcggaag gtcgttgcta tctgtcgtcg tatctgacgg
    14401 gtgagtcgcc caacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcagcaaa
    14461 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    14521 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    14581 acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag ttaatggcgg
    14641 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    14701 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcatac
    14761 cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa accactcttg
    14821 gcgcatatca ccgtaaatgg cagtgagaaa agcaatgaaa tattccttag ggccggtgct
    14881 gtggtattgg ccaaaagaga cgctggaaga tttttatcag caggccgccg ccagcagcgc
    14941 cgacgtgatt tatctcggtg aagcggtctg tagcaaacgc cgggcaacca aagttggcga
    15001 ctggctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtcc tctccacgct
    15061 ggcgctggtg caggcatcct cggaactggg cgaactgaaa cgctatgttg agaacggtga
    15121 gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgagc gcaaactgcc
    15181 gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa tattgctcaa
    15241 acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct
    15301 gcttaatcag tgcgatgagt tggggattcg caaccagttt gaagtggaag tcctgagcta
    15361 cggtcatctg ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgtcc
    15421 gaaagatgag tgcgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca
    15481 ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa
    15541 cctcggtaac gagctggcat ccatgcaggg cctggttgat gtggtccgcc tgtcaccgca
    15601 gggtactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgcgcc
    15661 actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg ctgggctgga
    15721 actgcaagcc tgaacaaata gctcactttg ttaacaactt taactactct ttaatgcagt
    15781 attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg
    15841 ctactcgatc tggcccctat ccccgaaggt tcttcagcgc gagaagcgtt ctcccactct
    15901 ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcggaacac
    15961 cacaatatga ccggcattgc cagtgctgca acatcggtat taattggtta tctggcagcg
    16021 aataccacca cgctgcatct ggggtccggt ggcgtgatgt tgcctaacca ctcaccattg
    16081 gtcattgccg aacagttcgg cacgcttaat acactctatc cggggagaat cgatttgggg
    16141 ctgggccgcg cgccgggtag cgatcagcga accatgatgg cactgcgtcg tcatatgagt
    16201 ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc
    16261 gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg
    16321 ctaggctcca gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt
    16381 gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc
    16441 aaaccgtcgg cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc
    16501 gacagcaacc gcgacgctga atttctgttt acctcgatgc agcaagcttt tgtgaagctg
    16561 cgccgcggcg aaaccgggca actaccgcca ccgattcaga atatggatca gttctggtca
    16621 ccgtctgagc agtatggcgt gcaacaggcg ctgagtatgt cgctggtggg cgataaagcg
    16681 aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtt
    16741 aacgggcaga ttttcgatca ccaggcgcgc ctgcattcgt ttgaactggc gatggacgtg
    16801 aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg
    16861 ctgtcctacg tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt
    16921 cggataaggc accgctgatt actgatacac cggcagcaaa ttaaagctcg ataagatatg
    16981 gaccagtgcg ttgccaacgc caaacaccag aatcagcgta atcatcggct tgccgcccca
    17041 gacacggaat ttcgggctgc caaagcgttt acgcgatgca cgggctaaca gcgccggaac
    17101 aattgccgcc cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccgtt
    17161 cgggaacagc agccccccca caactggcgg ggcaaaggtc agcaatgccg ttttcaagcg
    17221 gcccacagct gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt
    17281 tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag
    17341 gctacggctg ttcagcacgc cacttaatgc ctgtaccagc acatcaatgt taccgccctt
    17401 ctcggcgatg ccaataaact ccggacgagg aatgttgccc atcgtcgcca gcaaccagat
    17461 ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg
    17521 atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag
    17581 gcagaacggc aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt
    17641 gaacaatgtc gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat
    17701 gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac
    17761 cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga
    17821 catctctgcg aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag
    17881 gatatagagc acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa
    17941 atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat
    18001 catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa
    18061 aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc
    18121 gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc
    18181 catgagggct tctctccagt gaaaaatagt gcgactacgt tgttatgtat tgcactgtac
    18241 cagtacacga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat
    18301 attattttac agattgtttt cgctgttcag cgatgattac gcatcgccac cgaaacgacg
    18361 acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg
    18421 acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg
    18481 acgttcgcca ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc
    18541 atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg
    18601 cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt
    18661 gatgttacca atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg
    18721 acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca tatcgccaac
    18781 atcacgacgt tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg
    18841 atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc
    18901 tggcggtacg atcagagtac gttcaccctg tgccattttc agcagtgcgg cggccagagt
    18961 ttcgaggtcc agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta
    19021 ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg
    19081 gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt
    19141 acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacaaca gcgcgcggcc
    19201 agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatagg
    19261 gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc
    19321 ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct gctcacgcag
    19381 cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct caagagcttc
    19441 agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc
    19501 cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag tccagtagct
    19561 ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct gcggctcttt
    19621 cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt
    19681 ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg
    19741 cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc
    19801 acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg
    19861 cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca catttacgcc
    19921 gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt
    19981 cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg
    20041 taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc
    20101 attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc
    20161 gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc
    20221 agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa
    20281 attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac
    20341 aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac
    20401 gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat
    20461 tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg
    20521 aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag
    20581 ttggtttcac tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt
    20641 tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac
    20701 tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg
    20761 agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga
    20821 taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc
    20881 ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct
    20941 tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact
    21001 tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc
    21061 agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg
    21121 gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa
    21181 gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct
    21241 gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc
    21301 cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt
    21361 taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc
    21421 ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg
    21481 atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg
    21541 taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac cagaccttct
    21601 ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg
    21661 tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc
    21721 gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat ttcgatggtg
    21781 gtgccagttt cttcggtcag agcacggatt acagaaccgc ctttaccgat aacatctttg
    21841 atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca
    21901 cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc
    21961 tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc
    22021 agtgcagaga taccgtcgcg ggaacctgca actttgaagt ccatatcgcc caggtgatct
    22081 tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt
    22141 gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg
    22201 ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt
    22261 acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg cttcgccaga
    22321 cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag
    22381 tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca
    22441 agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca
    22501 cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca
    22561 cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga
    22621 acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg
    22681 tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc
    22741 tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg
    22801 cgcgcgttta gcgcttcgtt taccggctcc ggctgccagt cccaacgcgg tttaccggct
    22861 tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact
    22921 actgcgccca gcatctggtc ttcgctcagc agttgagctt cagattcaac catcagtaca
    22981 gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc
    23041 gggttcagta cgtactggtc attgatgtaa cctacgcggg cagcaccaat cgggccattg
    23101 aacggaatac cagacagaga cagcgctgcg gaagcaccaa tcatcgcgac gatatccggg
    23161 ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct
    23221 tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg
    23281 cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc
    23341 tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca
    23401 acaacggtaa cgaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga
    23461 cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg
    23521 atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg
    23581 ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt
    23641 gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa
    23701 tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc
    23761 ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa
    23821 cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc
    23881 agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg
    23941 ttgatctgtg cagtcagcag tgctacctga acttcggtag aaccggtgtc gtttgcgtca
    24001 cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc
    24061 caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg
    24121 caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacctgtt
    24181 atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg
    24241 gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt ccgtgacgcg
    24301 aaccagtcct tccagtggcg caccagatgt acgaaccggg ttaccatttt tgaagtaaac
    24361 agaagacgtt aacggaagat tcaccaccgg atagtccgaa gctggactgt ccattggcat
    24421 cagtaatgga tcaagtaact ccgcggctgg aatatcctgc tgttcagctt gctcaacaag
    24481 ttcacgcagg tgctccaggg tcaccatccg ttcaaccgga tatttactta ccgccagacg
    24541 gcgcaggtaa ataacatgcg cgccacagcc gagtttttca cccaggtcat caatgatggt
    24601 gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcatggcg
    24661 aataaacagc aattcataaa cggtaatcgg acgcgcttca cgcggaactt caatgccctg
    24721 acgcgcatat tcgtacagtt ttttgccctg atatttcagt gctgaataca tcgaagggat
    24781 ctgttcgata tcgccacgga aagtatccag tgccgctgcc agctgctctg cgctaaaggt
    24841 taccggacgt tcttcaacga tctgtccgtc ggcatcagaa gtatcggtac gctgtccaag
    24901 acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc
    24961 ttccccgagg caaatcggca acatgccggt cgccagcggg tccagcgcac cggtatgccc
    25021 ggcacggttg gcgttatata tacgtttcac tttttgcagc gcatcgttgc tggacatacc
    25081 ctgaggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact
    25141 cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg
    25201 ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc
    25261 agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa
    25321 gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac
    25381 gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc
    25441 atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc
    25501 atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac
    25561 aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt
    25621 gacctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgca gcgtaaacgc
    25681 cttatcccgc atggaaccct aaaaacctta agcaatggta cgttggatct cgatgatttc
    25741 gaatacttcg atcacatcgc cagtgcgaac gtcgttgtag ttcttaacgc cgataccaca
    25801 ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg actccagctc
    25861 gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac gtttaaccac
    25921 accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga acacgtcacg
    25981 aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac cgctcatcgc
    26041 cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat ccaggctttc
    26101 cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttaaagccaa ccaggatggc
    26161 gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc cagaaccgat
    26221 gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga tcgcttcgac
    26281 agaaccctgt acgtctgcct tcaggacgat attcacttcg tgaacttcgc cttcggtcat
    26341 gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa
    26401 tttaccctga cgatagagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac
    26461 ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa tggacggacc
    26521 cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa
    26581 gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac
    26641 cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg
    26701 taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca gttcatcgat
    26761 accggtaccc gctttcgcag atacgtgtac gaactggctt tcaccgcccc actcttccgg
    26821 caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcagctt ctggtttatc
    26881 gatcttgttc actgcaacca ccaccggtac ctgcgccgct ttcgcgtgct ggattgcttc
    26941 gatggtctgc ggcatcacac cgtcgtcggc agcaacaacc aggactacga tgtccgttgc
    27001 ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt
    27061 gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc
    27121 ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc
    27181 aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg
    27241 gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt tatggcccat
    27301 ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc
    27361 gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag
    27421 ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct gagcaggctt
    27481 ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc cacgtactgc
    27541 tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg
    27601 cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc
    27661 ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat cttcagtcgg
    27721 ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg
    27781 acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct tgagctctgc
    27841 agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg
    27901 attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag cttcacgctt
    27961 agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc
    28021 tgcctcacga cgggcttgct cttccgcttc acgctgcgct tgctcttccg ctgcaaggcg
    28081 ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac
    28141 cgatttgctt tttccaccgg taccaggaat gttaagggtg ctgcgtgttt tacgttgcag
    28201 cgtcaatttg tccgggcctg aatttttctg attcaggtgg tcaatcaaag tctgtttctc
    28261 ttgtgcagac acagagtcgt cagcagactt ccggatacct gcatcagcaa attgctgtac
    28321 caggcgttcc acggaggtct gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat
    28381 gctgttcctt cctgctacag tttattacgc ttcgtcaccg aaccagcaaa tattacgggc
    28441 agccataatc agtgctccgg ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc
    28501 aatgccctgt tcggcgagat cttccagcgt acaaacgcca cgggcggcca gtttgaatgc
    28561 caaatcacga tctacccctt caaggttcag cagatcgtca gccggtttgt tatcaccgag
    28621 gctttcttcc tgggcctgtg caatggtggc cagtgcattt ttagcacgct cgcgcagtgc
    28681 ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc tctttcatcg gcacataggc
    28741 cagttcttcc agcgtcgaga agccttcttc taccagaaca gtcgcgaagt cttcgtcgat
    28801 gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg
    28861 caggtcgtca acggtcatca cgttgagttc ccaaccgctc agctgcgaag ccagacgcac
    28921 gttctgaccg ttacggccaa tcgcctgcgc caggttaccg gcttcaacgg cgatatccat
    28981 ggtgtgttta tcttcatcca ccacgataga agcaacgtct gccggtgcca ttgcgttaat
    29041 cacgaactgc gccgggttat catcccacag gacgatatcg atacgctcgc cacccagttc
    29101 agtagacacc gcctgaacac gcgcgccacg catacctacg caagcaccta ccggatcgat
    29161 acgtttatcg ttggttttca ccgcgatttt cgcacgagaa cccggatcgc gagccgctgc
    29221 tttaatttca atcacttctt cgccgatttc tggcacttca atacggaaca gttcgatcag
    29281 catttccggc ttggaacgag tgacgaacag ttgcgcgcca cgcgcttccg ggcgaacgga
    29341 ataaagcacg ccacgaacgc ggtcgccagg gcggaagttt tcacgcggca gcatatcttc
    29401 acgcaggatc acggcttcag cgttgttgcc cagatccaga gagatgttgt cgcggtttac
    29461 ttttttcacc acgccggtga tgatttcacc ttcgtgttca cggaactgat caaccaccat
    29521 cgcacgttcg gcttcacgca ctttctgcac gataacctgt tttgccgtct gggtagtgat
    29581 acggtcaaag gtaacagact caatctgatc ttcaacgtaa tcgcccaggt tcaggctttc
    29641 atcttcataa cgtgcggctt caagggtgat ttccttggtc ggctgggtga cttcatcaac
    29701 aactaaccag cgacggaagg tgtcaaaatc accgcttttg cgatcgatct gtacgcggac
    29761 gtcgatctct tgttcatatt ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc
    29821 gaaaatcttc tcgcgaggta gcgccttttc attggatacg gcttcaacta cagccaaaat
    29881 ttctttgttc atcgcgggct tttcacctca tccagactat taaaagtggg gaaccaggtt
    29941 cgccttctgg atattactca gcgcgaacac ttcatcttta ccttcgacgg taactgtgat
    30001 catttcaccg tctaccgctt tgataacgcc ctgccattta cgacggtttt gtaccgccat
    30061 acggagaacc agagtcacct cttctccgac aaaacgggcg tagtgttcag ccgtgaacag
    30121 tgggcgatcg agacccggtg aggagacttc caggttataa gcaacggtga tgggatcttc
    30181 aacatccagc acagcactta cctggtggct cacatcagca caatcatcaa cattgatgcc
    30241 atcttcacta tcaatataga tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc
    30301 aaccagttca aaacccaggg cctcaactgg cgcagtaatc atctctgtta atttttgctc
    30361 taatgtggac aagcccaccc ccaagacata aaaaaagggc ctaaagccca gttattctgt
    30421 agtcagataa caaaaaaccc cgataaatcg gggctttata taactgaacc ctataaccgc
    30481 aactgcggtc tggagcactt tccagaagga ttttttcaaa tcccactacg aaggccgaag
    30541 tcttcacagt atatttgaaa aagaactcta agggaaa
//
LOCUS       DACAPB010000036        30034 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.036, whole genome shotgun sequence.
ACCESSION   DACAPB010000036 DACAPB010000000
VERSION     DACAPB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.036"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(222..698)
                     /locus_tag="IT904_001462"
     CDS             complement(222..698)
                     /locus_tag="IT904_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191266.1"
                     /translation="MLSGKELGRAIEQAIDKKLSIGSAKSKAEIARHFKIKPPSIHDW
                     INKGSISKEKLPELWNYFSDVVGPEHWGLKGYPLTDTCEPATDPIVKNGSIDELYNKA
                     SREKKAIIDFVLLEQGQRIPGWVDSDAKAYLDSLEMKIRRWAEQEEDGKKQTKARA"
     gene            800..1063
                     /locus_tag="IT904_001463"
     CDS             800..1063
                     /locus_tag="IT904_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3191267.1"
                     /translation="MRSSLEAISEACRIVGGQAALSRNLGISSPTVNQWTTGIRQIPA
                     ERCPAIEKATGGAVTCEELRPDIDWAYLRGAAMRKLNVTASNL"
     gene            1092..1544
                     /locus_tag="IT904_001464"
     CDS             1092..1544
                     /locus_tag="IT904_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191268.1"
                     /translation="MNLKEVVKSMCKAYPGGREAMAGALGMTVTQFNNNLYEKNGCRF
                     FEVSELEAMEDISNTSHLAEYFARRRGALLVDVPHLEELDRVDLFSRAMRTSAARGQV
                     DQIIEQALEDGVIERHEAEEIMVHHRRHLAAREEEIAAIITLFSRKKK"
     gene            1689..2411
                     /locus_tag="IT904_001465"
                     /pseudo
     CDS             1689..2411
                     /locus_tag="IT904_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     gene            2421..3263
                     /locus_tag="IT904_001466"
     CDS             2421..3263
                     /locus_tag="IT904_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3191269.1"
                     /translation="MKLTLPFPPSVNSYWRAPSKGPLKGRHLVSETGRKFQQAARAAI
                     IEQLRAVPRPSSDLAEVHINVVEGAGLELDPDDDSQVLKAIGGGRLLNIVTFTKSGTY
                     TPTKGTKRVRVKVWGAGGGGQNAPVSVGASGGCAGGFSEGLFNISDNETISVTIGTGG
                     SAVAAGVSSKGGNGGDTRFGSLISATGGSGGGATVPAGGIGSGGNILNITGGISQGGL
                     YYGSDAFIGGAGGSAFSSTGGNGHFGSSGDDGGFPGSGGAGGNGNYSSGKGANGLVYL
                     EEFS"
     gene            complement(3242..4333)
                     /locus_tag="IT904_001467"
     CDS             complement(3242..4333)
                     /locus_tag="IT904_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP3191270.1"
                     /translation="MLGLIRFFLASCVIAFHLTARIPALGNFAVNCFYVISGFLITYI
                     LHETYKFNFSMFWKNRILRLFPAYIFFLVMGFLIIKLIPSAKEFHSNWTGNFLPGDLL
                     GNLLIFPWAFLSDNAVANPFGAFSSIYHFAIDGNRFRIVTSSWSVGVEITCYFLLWLF
                     IARNKFTAITSILLSLLYHAYVYVVHHSFDMAYFPFLAATLPFSMGSLGYFAHRKFKA
                     MYLSPHKAFLITFICIGIFITNWHLYTINPLGQYNIILYYTNNVIALFTTLVLLKIKT
                     NIHLEKILKWFGDLAYPIFLCQYFGGFLAWLAIGGENRGLSIFLLGYPISIALGIVCV
                     ILIDKPLIKIRAKIRADAQSKNNQENSSR"
     gene            4698..6692
                     /locus_tag="IT904_001468"
     CDS             4698..6692
                     /locus_tag="IT904_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191271.1"
                     /translation="MDKRYNTGNPRPSNSMKDLNDNALAYDDFLNSESDTFIDRFGNA
                     QDTIIGATKKMAAATDAVIDEARQNLIPLSRQYMTLAAAQADIANIPAGSTTYVRSQD
                     GSSLADEYINLAGTLQPTGRRMVRDDYAYQVSPDSVTLAAYDPETSRVAPFLNTSGRL
                     IQIGPDGKYYELLTQQESELYALGREGSIPQFIGGEKVWRMTVDSTTNQIVEAYTVGG
                     KHWIYSDGGLVAVNNGNGGGGGDDDANQLPEYGLHLSGSTVYPYSETVPVCFIFVTAG
                     QSNARGYCPDADQTIVAATPIYPDNAFMLSGGVRRTGTRSTTLVPLVEAVSGTDKETA
                     ASGLANTFIRDMAAATGIMPRTLSIVCAQSGQAYEYQKRGNQVYQYLLDSIEDCVTAC
                     KARGWLPIVLCVDWMQGESDEDWSGLREGMYESRMRQYQRQITSDIIARTGQNEPPII
                     AITQLGYVNDGHGAFTGQYARLASTRLHGKEQFRLVNSLYQYDFISDGLHLTCAGQNR
                     RGAAVARALLQEWFTSGWSGMVPTSFVWNSPTQIQINVPAYTNLVLDTTTINTSGLAN
                     YGFSYTDETGAPPAISSIAISSDGKGVLINLATAPSGRFGRVSYATAENPLQSGASVK
                     PSGRTLGARGCVRSSAGIIWVYDTSVTLYDWLPAFRINVF"
     gene            6702..7502
                     /locus_tag="IT904_001469"
     CDS             6702..7502
                     /locus_tag="IT904_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191272.1"
                     /translation="MRLIYTLTGLNNPLLPVYSQTAAEKAISELSPSVWTPVKTEFLK
                     MGSGAKVVAISNRLDGGLFKSLETLEPSTKLNGSVLQGLNFSGASGMAGDTAVVMDAN
                     INTFAFIYQLPGGALPATPQDRAVIATQESTPHGVGIRTTSAGSFPIFLNGGIEADLA
                     FTPNNIGQSLFCAVVMCSNKAAGSYAIAYQRSDQSAVTSRQVTGYTIPAYSPSQKMSI
                     GGAGNGSVSPLTSVLSECLVFPGKYAYGTSTLDVILAYLMERIGKITA"
     gene            7513..7695
                     /locus_tag="IT904_001470"
     CDS             7513..7695
                     /locus_tag="IT904_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004892503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hin recombinase"
                     /protein_id="HAP3191273.1"
                     /translation="MHPSRSAAMEQERVGGRRRIMTEEVVERCRRMLENGATRQQVAD
                     VTGVDVKTIYKYLPAT"
     gene            8854..9086
                     /locus_tag="IT904_001471"
                     /pseudo
     CDS             8854..9086
                     /locus_tag="IT904_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V"
     gene            9043..9414
                     /locus_tag="IT904_001472"
     CDS             9043..9414
                     /locus_tag="IT904_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191274.1"
                     /translation="MSSRSSYTTQTEGYNVGFPSPAADYVERRLTVDSLCGTGPNTRI
                     VQTETGYAVVDCFVKPKQGDTVLIQYGGGTDFAKIMGRAFITRDGEALEGEALDDVTV
                     VGVVTFVINRIGKDDDDYPVI"
     gene            9610..9746
                     /locus_tag="IT904_001473"
     tRNA            9610..9746
                     /locus_tag="IT904_001473"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(10316..10846)
                     /locus_tag="IT904_001474"
     CDS             complement(10316..10846)
                     /locus_tag="IT904_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAP3191275.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(11189..11839)
                     /gene="zinT"
                     /locus_tag="IT904_001475"
     CDS             complement(11189..11839)
                     /gene="zinT"
                     /locus_tag="IT904_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAP3191276.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDSESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(12096..12731)
                     /gene="msrQ"
                     /locus_tag="IT904_001476"
     CDS             complement(12096..12731)
                     /gene="msrQ"
                     /locus_tag="IT904_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAP3191277.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(12732..13736)
                     /gene="msrP"
                     /locus_tag="IT904_001477"
     CDS             complement(12732..13736)
                     /gene="msrP"
                     /locus_tag="IT904_001477"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAP3191278.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPLAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(13845..14258)
                     /gene="hiuH"
                     /locus_tag="IT904_001478"
     CDS             complement(13845..14258)
                     /gene="hiuH"
                     /locus_tag="IT904_001478"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAP3191279.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            14391..15062
                     /gene="hprR"
                     /locus_tag="IT904_001479"
     CDS             14391..15062
                     /gene="hprR"
                     /locus_tag="IT904_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAP3191280.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            15062..16420
                     /locus_tag="IT904_001480"
     CDS             15062..16420
                     /locus_tag="IT904_001480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAP3191281.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(16528..17379)
                     /gene="hchA"
                     /locus_tag="IT904_001481"
     CDS             complement(16528..17379)
                     /gene="hchA"
                     /locus_tag="IT904_001481"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAP3191282.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(17970..19184)
                     /locus_tag="IT904_001482"
     CDS             complement(17970..19184)
                     /locus_tag="IT904_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP3191283.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDKSYARIGFKGETQIADQFTGYGQWEYNLQANGTEGDGDNSATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFMNGRTNGVATYRNNGFF
                     GQVDGLNFALQYQSNNESGSDGLFGQEGSGSGDGRSLAKENGDGFGMSTSYDFDFGLS
                     LGAAYSNSDRSNNQAARGYGDGNHFYGNSYAGGGTAEAWTVGAKYDANNVYLAAMYAE
                     TRNMTAYGSSDSHSADGKLGGGGIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLG
                     GQEMYNNGNYHYTNKDLVKYVEVGATYYFNKNMSTYVDYKINLLDNDDDFYANNGIAT
                     DDIVAVGLVYQF"
     gene            19750..20115
                     /locus_tag="IT904_001483"
     CDS             19750..20115
                     /locus_tag="IT904_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP3191284.1"
                     /translation="MEKCDFYHIIVLSLSFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            20155..20850
                     /locus_tag="IT904_001484"
     CDS             20155..20850
                     /locus_tag="IT904_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP3191285.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            20917..22335
                     /locus_tag="IT904_001485"
     CDS             20917..22335
                     /locus_tag="IT904_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP3191286.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSL"
     gene            22316..22786
                     /locus_tag="IT904_001486"
     CDS             22316..22786
                     /locus_tag="IT904_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAP3191287.1"
                     /translation="MADVHYKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKISKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22775..23695)
                     /gene="yedA"
                     /locus_tag="IT904_001487"
     CDS             complement(22775..23695)
                     /gene="yedA"
                     /locus_tag="IT904_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAP3191288.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            23868..24785
                     /locus_tag="IT904_001488"
     CDS             23868..24785
                     /locus_tag="IT904_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAP3191289.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            24864..25046
                     /locus_tag="IT904_001489"
     CDS             24864..25046
                     /locus_tag="IT904_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAP3191290.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            25284..26978
                     /gene="yedQ"
                     /locus_tag="IT904_001490"
                     /pseudo
     CDS             25284..26978
                     /gene="yedQ"
                     /locus_tag="IT904_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(26975..27790)
                     /locus_tag="IT904_001491"
     CDS             complement(26975..27790)
                     /locus_tag="IT904_001491"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAP3191291.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(28088..28315)
                     /gene="yodD"
                     /locus_tag="IT904_001492"
     CDS             complement(28088..28315)
                     /gene="yodD"
                     /locus_tag="IT904_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAP3191292.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            28424..28666
                     /gene="dsrB"
                     /locus_tag="IT904_001493"
     CDS             28424..28666
                     /gene="dsrB"
                     /locus_tag="IT904_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAP3191293.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            complement(28710..29324)
                     /gene="rcsA"
                     /locus_tag="IT904_001494"
     CDS             complement(28710..29324)
                     /gene="rcsA"
                     /locus_tag="IT904_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAP3191294.1"
                     /translation="MSMIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLLISS
                     KSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQMN
                     IKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(29614..>30034)
                     /locus_tag="IT904_001495"
     CDS             complement(29614..>30034)
                     /locus_tag="IT904_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAP3191295.1"
                     /translation="PVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNS
                     NAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTV
                     GISLMAALMPLIASFCEHLFSEIFNLLADIISELPLI"
BASE COUNT         8059 a         6671 c         7431 g         7873 t
ORIGIN      
        1 atttttaaca tgttcaatgt gatttttaag acgtactttg aagttttcca tgcatcctcc
       61 atgcaaagtg aaaacctgct gttaaatcaa agcaagtcac aatcccggat aggcttcata
      121 caagccaatc ccccacaggg attgagttat gcaagcgaat caatctcaga gcttttaaca
      181 tgtatggagc ttagagatat tgtccgttag accatataag cttaagctct ggctttcgtt
      241 tgtttttttc catcttcctc ctgctctgcc catctcctta tcttcatctc taatgagtct
      301 aaatatgctt tagcatcgct atctacccag ccaggtatac gctgtccttg ctctaagagg
      361 acaaaatcaa tgatagcctt tttttctctc gaagccttat tatagagctc gtcaatagaa
      421 ccatttttaa ctatgggatc tgttgcgggc tcacatgtat cagttagcgg gtatcccttt
      481 agtccccagt gctcggggcc cacaacatca gaaaagtagt tccaaagctc tggtagcttc
      541 tctttcgata tggagccttt attgatccag tcatggattg atgggggttt tattttgaaa
      601 tgacgtgcga tttccgcctt actcttggca gaacctattg aaagcttctt gtctatggcc
      661 tgctcgatcg ctcggcccaa ttctttacca ctaagcattg cctaataatc ctcataacct
      721 atagcttagg caattcctat tggttgttta ttaggcttag cctaatatct gcttgtgtgg
      781 aaatcatagg aatccgttta tgagaagtag ccttgaagca atcagtgaag cctgccgcat
      841 tgttggggga caagccgctt tgtcaaggaa tctaggcatc tcatcaccaa cagtgaatca
      901 atggacaacg ggcattaggc aaatacctgc ggaacgatgt cctgcgattg agaaagctac
      961 tggtggtgct gtcacctgcg aagagcttcg tcctgacatt gactgggcct atttaagagg
     1021 tgcagcaatg cgaaagctta atgtcactgc atcaaatttg taactaccac ccgagtttga
     1081 aaggagtagg tatgaacctc aaagaagtcg tgaaatctat gtgcaaagca tatccaggtg
     1141 ggcgcgaagc aatggctggc gcactgggaa tgacggtgac gcagtttaac aacaaccttt
     1201 acgagaaaaa cggctgtcgt ttcttcgaag tcagcgaact ggaagcgatg gaggacatct
     1261 cgaatacatc tcacctggct gaatattttg cccgccgtcg tggtgctctg ctggtggatg
     1321 ttccgcacct ggaagaactg gaccgcgtgg acttgtttag ccgggcaatg cgtacctctg
     1381 ccgccagggg gcaggttgat cagattatcg aacaggcgct tgaagatggc gttattgaaa
     1441 ggcacgaggc cgaagaaatc atggtgcatc accgccgtca cctggcagct cgtgaagaag
     1501 agattgccgc aatcatcacg cttttttcac gcaaaaagaa gtgacgccag cgagttgcag
     1561 ctcctggcgt cgtggcgtgt cgttatcagt ggagattact aacgcatgaa cagtttatca
     1621 acacaatacc gcaggtcgca acttgtagcg cggccagttc ctggtggagc agggccggtg
     1681 cagttcgtgt agagccattc gttggtggtg ggtcggtatt cctgaacagc gaaaagcacg
     1741 cagattacct gctggcggac gttaacccgg acctgattaa tctgtatcag atgttagcgg
     1801 tcgtgccgga tgaagtggaa ttgaaggccc gctggatgtt cgagcacatg cggtcaccag
     1861 agcgctatga gctgatccgt tccgagttca actctcagac gctggatgct actgaacgcg
     1921 cagctgcatt cctgtatctc aaccggcatt gcttcaatgg tctgatgcgc tacaaccagg
     1981 cgaacaagtt caatgtgggc tggggaggct acaaggctcc gtattacccg atggatgaga
     2041 tgaaagcctt cgcggctatg gcgcataact gcgtattcat gacggctgat taccgccgga
     2101 caatcagcct ggccgggaaa ggggatgtgg tttactgcga tccgccttac gaaccgatgc
     2161 cgggaacaac cggattcacc gcctacgccg ctggtggttt taactgggag aaccaggtag
     2221 acctggcgaa gcaatgcgta tcagcctttc accgtggggc tcgggtagtg atttctaact
     2281 catctgcacc gaaggttctc gacctgtacc gggagcatgg ttttaacctg caattcatca
     2341 acgcgcgccg ttcgatctcc tgcaaaagca gtacgcggga agtcgcaaaa gacgttgtag
     2401 cgatccttta agggggctaa atgaaactga ctttaccatt tccaccgagc gtaaatagtt
     2461 actggcgcgc cccgagcaag ggaccgctga aaggcaggca tctggtaagc gagacagggc
     2521 gcaagttcca gcaggcagcg agagcggcga ttattgagca actgcgggcc gttccccggc
     2581 catcctcaga tctggccgag gttcacataa acgttgttga gggcgcgggg cttgagcttg
     2641 atcctgatga tgattcacag gttcttaaag cgattggtgg cggtcgcctg cttaatattg
     2701 tcacctttac taagtcaggg acttacactc caactaaagg gacgaaaaga gtgcgggtga
     2761 aagtttgggg agcgggggga ggtggccaga atgctccagt tagtgtgggc gcatctggag
     2821 gttgtgctgg tggattttcg gaagggttat ttaatatttc tgataacgaa actatatctg
     2881 tgacgatagg tactggtggc agtgctgttg ctgctggtgt ctcgtctaaa ggcgggaatg
     2941 gtggtgatac aaggttcggg agtttaatct cagctactgg agggagtggt ggcggggcta
     3001 cagttcctgc tggaggtatc ggaagtggcg ggaacatttt aaatattact ggcggaatat
     3061 ctcaaggtgg tctgtattat ggttctgatg cttttattgg cggggctgga ggatctgcat
     3121 tttcatcaac gggtggcaat ggccacttcg gtagttctgg cgatgatggc ggtttccccg
     3181 gctcaggtgg tgccgggggg aatggtaact attcatcagg aaaaggagcg aatggtttag
     3241 tttaccttga agagttttcc tgattatttt tgctttgagc gtccgccctt atttttgctc
     3301 taattttaat tagtggcttg tctattaata ttacacaaac gatcccaagt gcaattgata
     3361 ttgggtagcc taataaaaag atggataagc cacgattttc accaccaata gcaagccatg
     3421 ctagaaatcc accaaaatat tgacaaagaa aaattggata ggccaggtca ccaaaccact
     3481 tgagtatttt ctcaagatgt atgtttgtct ttatttttaa taataccagg gttgtaaaca
     3541 atgctattac attattggtg tagtaaagta ttatgttgta ctgacctaat ggattaattg
     3601 tatataaatg ccaattggta ataaaaatcc cgatgcaaat aaatgttatt aaaaaagctt
     3661 tgtgtggaga aagatacata gctttgaact ttctatgggc aaaataaccc agagaaccca
     3721 tgctaaaagg aagtgttgca gcaaggaatg ggaaatacgc catatcaaaa gaatggtgta
     3781 ctacatatac gtaagcatga taaagcagtg aaagcagaat cgacgtaatg gctgtaaatt
     3841 tatttcgggc tatgaaaagc cacaataaaa aataacatgt tatctcaaca cccactgacc
     3901 atgatgatgt gactattctg aatctattac catcaatagc aaagtgataa atggatgaaa
     3961 aggcaccgaa tggatttgct acagcattat cagaaaggaa agcccagggg aagattaaga
     4021 ggtttcctaa taaatctcca ggtaagaaat tgcctgtcca gtttgagtgg aattcctttg
     4081 cggatggaat taattttata ataagaaagc ccatgacgag aaaaaatata tatgccggga
     4141 ataacctcag tattctattt ttccaaaaca tagagaagtt gaacttgtat gtttcatgca
     4201 aaatgtatgt tattaaaaaa ccactaataa catagaagca attcactgca aagttgccaa
     4261 gtgcaggtat gcgagccgtt aagtgaaaag caatgacgca cgatgccaaa aaaaacctta
     4321 ttaggccaag cattatttta tctccgcgcc aatatcgcgc attttattta ttacattgtt
     4381 aagttgctca tcagacagaa caagctgagc tgcaatataa aacagtatgt gtcttgacat
     4441 taccctgggg ctctctccgc cagtgtattt ccgccattgg ctatcgctgg ctacacctgc
     4501 gagccctgcc atttggtttc cggagtaatt tagtgcggat ttcagtctgg ctaaatcttc
     4561 ctgcgttgga ggggtgtatt tatttattaa aatcattctc ttatttaacc gtttaaacac
     4621 gtaaggcgat gttagccctg aaggggcggg gcgtcaatct taattgcttc attgaaaaga
     4681 tgaagacagg aacaggtatg gacaaaagat ataataccgg caatccaaga ccttcaaata
     4741 gcatgaagga tctgaatgat aacgccctgg cgtacgatga tttcctgaac agcgaaagcg
     4801 atacttttat agatcgtttt ggtaacgccc aggatacgat aattggggct actaaaaaaa
     4861 tggcagctgc taccgacgct gttattgatg aagcccgcca aaacctgatc cctctcagcc
     4921 ggcagtacat gacgctggcg gcggcgcagg cggatattgc gaatattccg gcaggttcaa
     4981 caacctatgt ccgcagtcag gacggaagct ctctggccga tgagtatatc aacctcgctg
     5041 gaacgctgca gccaaccgga cggcggatgg ttcgtgacga ctacgcatac caggtatcgc
     5101 cagacagcgt gaccctggca gcatatgatc cggagacttc ccgcgtggct ccatttttaa
     5161 atacaagcgg cagattaatt caaatcggtc ctgacggaaa atattacgaa cttttaaccc
     5221 aacaagaatc cgaactctat gcgctgggcc gggagggttc tataccgcag tttattggcg
     5281 gtgaaaaagt gtggcggatg acggttgatt caaccacaaa ccagatcgtt gaagcttata
     5341 cggttggtgg gaagcactgg atttactcag acggtggcct ggtagctgtt aataacggaa
     5401 atggcggtgg tggtggcgac gatgatgcca accagctccc tgagtatgga cttcatttgt
     5461 cagggtctac tgtgtacccc tactcagaga cagtgcctgt atgttttatc tttgtgactg
     5521 ctgggcaatc caacgctcga ggatattgtc ctgacgccga tcaaaccatt gtcgcagcaa
     5581 cgccgatata tcctgataac gctttcatgc tcagcggcgg ggttaggcgt acagggacac
     5641 gcagcactac tctggtgcca ctggttgagg cagtaagtgg gacagataaa gaaacggccg
     5701 caagcggcct cgcgaacacc ttcattcgcg atatggctgc agctaccgga atcatgccgc
     5761 gcacgctatc aatcgtatgt gcgcagtctg gtcaggctta cgagtaccag aaacggggta
     5821 accaggtata tcagtatctg ctcgattcaa tcgaagactg cgtaacggcc tgtaaagcac
     5881 gcggctggct gccgattgtt ctctgcgttg actggatgca gggagagtcc gacgaggact
     5941 ggtcaggatt acgagaagga atgtatgaat cacggatgag gcagtaccag agacaaatca
     6001 ccagcgacat catcgcaaga acgggtcaaa acgaaccgcc gattatcgcc attacccagc
     6061 tggggtatgt caatgacggg catggtgcat ttacaggcca gtacgcgcga ctggcgtcga
     6121 cgcgattgca cggaaaagag caattcaggc tggtcaatag tttgtaccag tacgatttta
     6181 tttcagacgg tctgcacttg acgtgtgcgg gccagaaccg gcgcggagca gctgtggcga
     6241 gagcgcttct ccaggagtgg tttacgagcg gctggtcagg gatggttccg accagtttcg
     6301 tgtggaactc acccacgcag atacaaatca atgtcccagc gtatacgaac ctggtgctgg
     6361 acacgactac gatcaacacc tccggtctgg ccaattacgg ctttagctac acggatgaga
     6421 ctggtgctcc acctgctata tcgagcatcg cgatcagctc ggacggcaag ggcgtgctga
     6481 ttaacctggc gaccgccccc tctggacgtt ttgggcgcgt ttcctatgcg acagcagaaa
     6541 acccacttca gagcggcgca tctgtaaaac cttccgggcg gactcttggt gcaagagggt
     6601 gtgttcgatc ttccgctgga atcatatggg tgtatgacac atccgtgact ctttacgact
     6661 ggctccccgc ttttcgtatt aacgttttct gaggataaaa aatgagactg atttacacac
     6721 taactggact gaataacccc ttactccctg tgtactcaca aacagcggca gagaaagcga
     6781 tctcagaact atccccatca gtatggacac cagtgaagac agagtttcta aaaatggggt
     6841 ctggtgcaaa agtagtagct atctctaacc gattagatgg cggacttttc aagtcactgg
     6901 agaccctcga gccatccaca aagctgaatg gttctgtttt gcaggggctt aatttctccg
     6961 gtgcatccgg catggctggt gatacggcgg tggttatgga cgcaaacatc aatacttttg
     7021 cattcatcta tcagctgcca ggtggggcgc ttccggctac gccacaggac agagcggtta
     7081 tcgcaaccca ggaaagcaca cctcatggcg tcggcattcg tacaacgtca gcaggttcat
     7141 tcccgatttt tctgaatgga gggatcgagg ccgatctggc atttactcct aacaatatag
     7201 ggcaatcttt attctgcgct gtagttatgt gctctaacaa ggctgctgga tcctatgcaa
     7261 ttgcatacca gcggtcagat cagtctgcag tgaccagccg acaagtgacg ggctatacaa
     7321 tcccagcgta tagcccgtct caaaaaatga gtattggcgg agcaggcaat ggttcagtgt
     7381 ctccgttaac atccgttttg tcagaatgtt tggttttccc cggcaaatat gcatatggga
     7441 ccagcacact ggatgtcatc ctggcatatc tgatggagag gattggaaaa atcacggcct
     7501 agaatttgcc gaatgcatcc gagtcggtcc gcagcgatgg agcaggagag agtcggcggc
     7561 cgccgccgga taatgactga agaagtggtg gagcggtgcc gcagaatgct ggagaacggc
     7621 gctacccggc agcaggtggc tgatgtgaca ggcgtggacg tgaaaacaat ctacaagtac
     7681 ctcccggcga cttgaaggca aagatttcac tacttttcct gatatgttac gtttggctta
     7741 atcaattcat tcagctttga aaacagtttg gtttgttcgt gaacggtaag aaaacaataa
     7801 gttttgagca atttttaact attaacagca atcttgtttc catctcagat acatgggctg
     7861 acttgtgggc gttaattttt cacacgggtt taagcgctgg aaggctgctg agtattcgat
     7921 atgatgatat tgatggtgac ttgatactga tacgaaaaca gggtcacctg aaggagctac
     7981 gtgttaaatc aacccctcca gtggagggga tcattgctcg tagaagagaa cgctatccag
     8041 aagatgtttt tttatttcag agccattcta accgtgtgaa gtaccaatgc cggccggtca
     8101 ctataattgc tttcaacgcc gctttacgtc gcgccgctag atcattacca gacgttaacg
     8161 taagcagtag tagcgcgaga aacataccgg actaaccgcc tgtccagtcg cgtgtggccg
     8221 atgtgacagg cgtgggggtg aagactattt acaaatattt gccagtacaa tacggcgata
     8281 aaaaccccct tgagcaggca cactcaaggg gaaaatacta cataacatca ttgctgtgtg
     8341 cgtctttgcg ctcgtctatc ttccaagaat atgcctaaag cttccagata tttctggtct
     8401 gagctgttac atcatggagt aggtgccgat gtgataggtt aagagcgaag atgatctgta
     8461 agtaccttcc gacgtcgagg ggcaatgacc atgaatttgg gtctatacca tcccaattca
     8521 tatattcttt gtaagtctat gaaatataga gcaaagtatt ctgttcgaaa tgaaccatat
     8581 ggaatagcca aaggctaaaa tgcccagagt aaaaacaaca atcagcaagt ccgtctgtga
     8641 catcttatat ccattttgca gtagcaggtt ttgagaaaag atagttcata gttggcacat
     8701 agacaacata atcactaagt gaaaccaata tcagaggcta aaaggtgact ggtttcctcc
     8761 tcagtgttcc tgattgatag ctggaacctg tattgatcag atctcttaat gaatctactg
     8821 tatataaaaa cagtattttc gggaggtgaa gttatgccgc gaaactcaga tatcgaaata
     8881 gcctggcgtc aggcaattgt cattgagcct aatggccgtc gcaccgtgac aacgtccggt
     8941 tttatccggg aactcgcaaa agttaactgg atatggtcac cgcgccaggc taaccagtgg
     9001 atagagcact gacattccgg gatgtctcaa cgcaggaagg cgatgagctc acgttccagt
     9061 tatacaaccc aaacggaggg ctataacgtg ggatttccgt cgccagctgc tgactatgtt
     9121 gaacggcgtc tgaccgttga ttcactctgc ggtaccggcc ccaatactcg gatagtacaa
     9181 acagaaaccg gctatgccgt agtggattgc ttcgtaaaac caaagcaagg agataccgtt
     9241 ttaattcaat acggcggcgg cactgatttt gcaaaaatta tgggccgggc atttattaca
     9301 cgagacggtg aagcgttgga aggtgaggcc ctggatgatg ttacagttgt cggggtagtg
     9361 acattcgtta tcaatcggat agggaaggat gatgatgatt atccagtaat atgaaaagtg
     9421 gtgcgcacct aaatttcatc cttacccctg gatgaagagc ctataaaaac atgctggtaa
     9481 agtagcgcca ttcgatactg gaaaggcact cgcaactcct ttcagaggcg aaagcattcc
     9541 cagccaatca ttaagggcta atgtcaaaat ttcatgagaa aatgaagttt tgaagatggc
     9601 taacttattg aaaaggttgt gtgtgaaatt cgaggtattt tctcagaaat acaggtaact
     9661 atctgttttt aaataaaagc agttcggtct cgaaaaccgg agtaggggca actctaccgg
     9721 gggttcaaat ccccctctct ccgccacttt atcaatgact tatctcccga cttcccgcct
     9781 tgcttttcct aaacagaaca atcgtagaat attcttgaag ggttagatcg tcactgtttt
     9841 ctgttcgata ctgtgacatt cagcacttga ttcgctatgg atctgacagg aaagtttcga
     9901 gcgaatatgt gcagttattc agtcgttttc ttatcggtca ccattcttct tttagacatt
     9961 catcctatga aactgctgta aagttggtgg tgggaactga agttgcgtag agaaggggtc
    10021 aatatttgag ccaaaaaagg aacgcggccg ttagattaaa gccgcgggac aaagtccata
    10081 atttccaaga gttgttgaat ttcccagctt ttttgaaagc ctttgtatca tactaacaag
    10141 taaaaaaggt ttaaatacca aaattaacct gtatttttga taagtcttac cattgtacga
    10201 tttacacagt ttctgctcct ccttgttgat agtggaaagg gtgcattgtg cacttgcgta
    10261 aacatttatg acgcgttgat gtccatggtt cacatttcct tgtcagggga tcgacttatt
    10321 tgcggcgagg catcattcgt aacagagtat tatctttctg aatgtagtga tgaaagagtg
    10381 ctgcgccagc gtgagctgcg attaaaaagt aacctatatt tgcccaggtt tcgtgaatat
    10441 tttttatcag tgctttaatt tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa
    10501 ggaaactcca cgattttcca ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa
    10561 ggaaggttat ataaagcatg atgtgcatta gcgaagccgc tttcatttgc caggcaggtg
    10621 gcggtggcac aatagaagga tcactataac ggtgttttat aatcaggcgt gaaaacatta
    10681 acacccaaac gaatataccc gcattgtaat gtgtttctcg catcagcaga taagtactac
    10741 tacctttagg aaaccagcca cggagttcca tcgcggcata ggttattgca acgaaaagca
    10801 gcgttatcca atgtaaataa atttgagttt ttgagaatcg gttcattttt gtagctcctg
    10861 taatttttac agtcaagcta tcccacgaac cttaaaaatt ccttatacag atgtggtttt
    10921 aagaatttgt tatttaaaaa attgatagtg cacattgtaa ccagccaata taagcaatgg
    10981 tgtgagcaca ggtatctttc tctgttggcc gtattgtgta tggaatccgt tattggttaa
    11041 ctcaatgcgg gttagcttat taatatacag acacgtcata gtaatgagcg tgtctgtagc
    11101 tagcttgcgt tcagtggcgt ttgtggatgg accagtggca gttatgatga cttactaaag
    11161 cggcatcgag gcgttatcat gagaatactc aatgagacat catttcctct accacttctt
    11221 cgctactcaa ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt
    11281 gctgtgaatc attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat
    11341 gatcgctaaa ttgtatatat ttaggggctt tgctttcaga atctttacat tcgaataagt
    11401 aacgaacgcc tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt
    11461 tacaggatgt tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca
    11521 tctcgatatc tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag
    11581 ttttatctgc atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag
    11641 gataaacgga ttgccagact ccatcccagt cactgagcgt tcggttttgt acattggcat
    11701 catcaaaaac accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt
    11761 gatgaccatg cgaaaaggca ggagcgctaa caataaagac acctaaagca acagccagtt
    11821 tgtgaagacg aatcgccaaa acagtttcct ccagagtcat tgtatatgat gtgtaatgtt
    11881 atattgtaac atatggcaaa atgcaacaac tcttaggaaa cgagagcatg gtattctcaa
    11941 gtgtattcag ctatgttatt gaaatagaat aaattattct ggagatgaga ggttgatgac
    12001 aaacgaaaca taacctgatg tgatctgcgc aggccggaca gggcgctcgc tccacatccg
    12061 gcagggataa cgcgtacttt gtcggcaata tgaagttata cagataattt gttgtgaacc
    12121 tgcttgcgta accggttaaa aagggaacgc aacttcttat accgtaaggc taaaagcagt
    12181 acagccagcc cagcgtagat gagcggctgc ggtgaaataa tcttcactga ccacagataa
    12241 tgtattgggg ccaggatcgc gacaagatag acgaaattat gcaactgttg ccagtgcttg
    12301 ccgagttttc gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa aataacccag
    12361 ctgataattc ctaacgttaa ataaggccgg gtaattaact ccttgcctaa taacgccaga
    12421 ttattcacgc ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag
    12481 caccataagc ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc
    12541 agaggtgtga ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc
    12601 tgaatatctt tcaccggatc ggcacccagt ccaccgtgat tgatcgccca gaccagccag
    12661 agaaacggca acaatccggc aagatgcagg ctaactttca gccatgtcac ctgttttgct
    12721 gtcagacgca ttcagaaatt ctcccgcaaa tccaggccac gatacagcga tgccacctgg
    12781 tcggcgtaac cattaaacag tagcgttggc tggcgctgaa catcgaggat gccgcctgaa
    12841 ccaataaatc gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg attaacgttg
    12901 gcgtaaaaac cgtattcgtc aggcgctgcc agattccagg tggttggcgg acgctcgcgg
    12961 gtcagcttaa tactaactat cgatttaatc cctttaaagc catatttcca cggcacaatc
    13021 agtcgtaccg gcgcgccatt ttgtggcggt aacgccttgc cataaacacc cacggtcatc
    13081 agtgtgagcg gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg atatttcagc
    13141 ccgccgccga taaagcggtc ctgctggcct ggcatttgtt ccggtgcata aattgtttcg
    13201 aaagcgacat acttcgcatt gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga
    13261 aaaccaatcc acggcaccac catcgaccat gcctccacgc agcgcatacg ataaatacgc
    13321 tcttccagcg ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg ttttgccact
    13381 tcgccgctga ttttcagtgt ccatggatcg gttttcaggc taccagcatt agcggcggga
    13441 tcggctttat ccagcccgaa ttcatagaag ttgttataac cggagacttt atctactggc
    13501 gtcagtggca ggttattttg ccaggcggca ggcttgctga actccagcgg ttttccggcg
    13561 ggggcgagcg ggcgatcgtt ccctttaaac cagctaagca gatcggcatg cgcagcgtga
    13621 ggcaaagaaa gtgcagctgc actgatgccc agtgctttta acacctgccg acgcttcata
    13681 aagaataccg actcggccgt aacatctgat tcttttaaaa attgattctt tttcataagc
    13741 catcacactt ttttgtcgtt agccagcaag agccgtttac tgttaatctg tcagggagtg
    13801 tcctccctaa cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag
    13861 gttgaatacc catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta
    13921 atatgaaatt caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag
    13981 tccccggttt taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt
    14041 gccttaattc gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttatcc
    14101 gccttctttt caagagtgac tgtcacgtca gcagcgggtt ttccggtttg ctggttcaaa
    14161 atgtgcacgc taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct
    14221 gccgttgcta ccgagagtac taaataacgc tttaacatgt ttatatcctt gtcatgtgaa
    14281 tgagttttga ggatagcgaa aaagcggcca gggtaacctt acagcagcat tacaattttg
    14341 taatgaacca ggctgtttct ataacatatg atttatggca tattattttc atgaagattt
    14401 tacttattga agataatcaa aggacccagg agtgggtaac gcaggggctt tccgaagcgg
    14461 gttatgtcat tgatgccgtt tctgatggca gagatgggct ttatcttgcg ctgaaggatg
    14521 attatgcatt gataattctg gatattatgc ttccgggtat ggatggctgg cagatcttac
    14581 aaacgttaag aacagcaaag caaacccctg ttatttgcct tactgcaagg gattctgtcg
    14641 atgacagagt cagagggctg gacagtggag caaatgatta tctggtaaaa cctttttcat
    14701 tttctgagtt gctggcaagg gttcgggcac aattaaggca acatcacgct ttgaattcaa
    14761 cattagaaat cagtggctta agaatggact ctgtaagtca aagtgtgagc agggacaata
    14821 tcagtattac actgacgcgc aaggagtttc agttactttg gctactggcc tccagagctg
    14881 gcgaaattat acccagaacg gttattgcga gtgaaatttg gggaatcaac tttgatagtg
    14941 ataccaatac ggtggatgtc gccattcgca ggctccgcgc aaaagttgat gatccttttc
    15001 ctgaaaagct aatcgccaca atccggggga tgggctattc attcgtagcg gtaaaaaaat
    15061 aatgaaaaga ttgtctataa ccgtccgttt aaccttgctt tttatattgc tgctgtctgt
    15121 tgctggcgct ggaattgtat ggactctcta taatggcctg gcaagtgagt tgaaatggcg
    15181 cgatgataca acactcatta accggacagc gcagatcaag cagttgttaa ttgatggggt
    15241 aaatccagat acgttacctg tgtactttaa ccggatgatg gatgttagtc aggatatctt
    15301 gatcattcat ggtgatggca tcaataaaat tgttaaccgg acgaatgtca gtgatgacat
    15361 gttaaataac atacctgcta gtgagacaat cagcgcagct ggcatttaca gaagcattat
    15421 taatgataca gagatagatg ctttacgaat taatattgat gaagtttcgc catcattaac
    15481 ggttactgtg gctaaattgg cttcagccag acataacatg cttgaacagt ataaaattaa
    15541 tagcattata atttgcattg tcgccattgt actttgctca gtattaagtc cgctgttaat
    15601 cagaacggga ttacgagaga tcaaaaagtt gagtggtgta acggaagcgc tgaattataa
    15661 cgatagccgg gagcctgttg aggttagcgc attaccgaga gaactaaaac ctcttgggca
    15721 ggcgttgaat aaaatgcatc aagccttagt caaagatttt gaacgcctaa gtcaatttgc
    15781 tgacgatctc gctcatgaac ttagaacgcc cattaatgca ttactgggtc agaatcaggt
    15841 tacgctcagt caaaccagaa gtatcgctga atatcaaaaa acaattgccg gtaacattga
    15901 agagctggaa aatatttcgc ggttaacaga gaacatactg tttcttgccc gggcagataa
    15961 aaacaatgtt ttggtgaaac tggactcgct ttctctcaat aaggaagtcg aaaatttgtt
    16021 ggattatctt gaataccttt cagacgagaa agagatttgc tttaaggtcg agtgcaatca
    16081 gcaaatcttt gcggataaaa ttttactgca acgaatgtta tcgaatctta ttgttaatgc
    16141 cattagatat tcaccagaaa aatcgcgtat tcatataacc agttttcttg ataccaacgg
    16201 ctatcttaat attgatgtcg ccagtcctgg aacgaaaatt catgagcctg aaaaactctt
    16261 ccgtagattt tggcggggag ataattcgcg tcattccgta ggtcagggac ttggtctttc
    16321 tttagtcaaa gcgattgccg aattacatgg gggaagtgct acgtatcact atctcaataa
    16381 gcataatgtg ttccggatta tgttaccgca aagaaattaa catagggctt cagtacgcca
    16441 taaatcaacc atgaaaaaac gcagccacgt cgtgagtatt aacgtggctg cgattgatta
    16501 tgcgcttaca ttcatacgta acagggatta acccgcataa gctgccagca tttcctgcgc
    16561 cgccagttta cccaacgcat tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc
    16621 cttatgtact cgcccggtga tgtcgtcatt aataatgttc atgcccattt tcttcagctc
    16681 ttcgccgaag taccaggtga gatgacccgg catatagcct atctctggcg tttgtttgtc
    16741 tgcggcgtct gggaatgcgc aaatggaata accattcagt gggttatcgc cgtggcgaag
    16801 cgccagaaaa gccgccgggc cgtggcaaag ggagataaca aaacggtcat ttttgattgc
    16861 ccactgcaaa gcagcagcta cgtcctggct ttctggtaaa ccaataagtg cgccatgacc
    16921 accaggaaca aagattgctg catattcgct gtcagcgttg aggctggcga caacatccgc
    16981 gagtttcttc ggattgcgga acaacgattt atgttgctca aagaatggca ttactttttc
    17041 atctttgtgc ggcatagccc agtattcaaa cttggtcatc agaccggaaa tggtcgccac
    17101 ttcgaattcg aaacctgcag catggagatg atacaacggc agcaacgttt caatcggatg
    17161 gttaccggtc gagaacagtt ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat
    17221 caccagaatt ttatgcttac cgcgatacgg ttttggatag tccacgccat caagatcaga
    17281 gacgggactg gtatattggc taagcgaata ttctgaaggg aagaatgcat tatcttcagc
    17341 aatatcgacc tgcggatttt tacttgtttg aacagtcata gtgtattcct tattgttgct
    17401 tagttagggt agtcactata tttgcgactg agctggtcgc tatttttaac cagtctggaa
    17461 aattgagtca atatatcgaa agcggtagtc ggcgggtgta agtatgtaat gatgatgctg
    17521 cactggtgtt gaatattatt cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg
    17581 gcctcaatga ttttagacca ttatcaaagg tacggggcgg cggggagaga tttagtgctg
    17641 tgaaatattg tggtactgat gaatatttat atcgcggatt ggtagagaaa agattatttc
    17701 atgcactatt tttgcaaata tttctatatg ggaagcgtgt ttgttacgga ttaattcata
    17761 aagaaatgca tttgggtgtt ggtttgaaga gtcgctggtt gatgttattg tgccagtagg
    17821 ggcagggtaa ggtgctgtat cgatatgcat tggcaattgc gtgtgccgca gatggagatg
    17881 aaatgccaat aaaaaagcct gaattcgatg agggacgaac tcaggctagt gtttagtttt
    17941 acagtaacac gccgcagcac gttggctgat tagaactggt agactaagcc cacagcaacg
    18001 atatcatcgg tagcgatgcc gttgtttgcg tagaagtcat cgtcgttgtc cagcaggttg
    18061 attttgtaat caacataggt ggacatgttt ttgttaaagt agtaggttgc accaacctca
    18121 acgtatttaa ccagatcttt attggtataa tgataattac cattgttgta catttcttga
    18181 ccacctaaat ccttaccttt agattggagg taagcaattg aaggacgcag gccgaaatca
    18241 aattggtact gagcaacaac ttcaaagttc tgagttttgt tagcgatacc accgccgcca
    18301 agtttcccat cggctgaatg agagtcgctg ctaccataag cggtcatatt gcgagtctct
    18361 gcatacattg ctgctaaata tacattgttg gcatcgtatt tagcaccaac agtccacgct
    18421 tctgcagtac caccacctgc atagctatta ccatagaaat gattgccatc tccataccca
    18481 cgagcagctt ggttattgga acggtcagaa ttagagtaag cagcacccag acttaaaccg
    18541 aagtcgaagt cgtaggaagt agacataccg aaaccatcgc cattttcttt agcaagacta
    18601 cggccatctc cgctgccaga tccttcttgg ccaaagagac cgtcactgcc actttcatta
    18661 ttactctgat attgcagcgc aaagttcaag ccatcaacct gaccaaagaa gccattgtta
    18721 cggtaggttg caacaccgtt ggtacgaccg ttcatgaaat tgtcggcacc agcataggta
    18781 tcgccaccaa attctggcag catatcggtc catgcttcga tgtcatatac gacaccgtag
    18841 ttacgaccat aatcgaaagt accattctga ccaaaaccta aacctgcgaa agccagacgg
    18901 gtagcagagt tatctccatc gccttctgtg ccatttgctt gcaggttgta ttcccactga
    18961 ccataaccag tgaattggtc agcgatctga gtttcacctt tgaaaccgat acgggcatat
    19021 gacttgtcgc catcgctgcc agcatcatca gagaagtagt gcaggcccgc tacttttccg
    19081 tacaaatcca gtttattacc atctttatta tagacttcag ccgcatttgc tgcgccagca
    19141 actaataacg ccgggaccag cattgccaga acttttcttt tcattatgta ttcccttgtg
    19201 attataatct tcatgaatat atcaataagt gccgttatcc aaaaaaagca catttggata
    19261 ctattctatg aagttcattt tattttaagg attacatgta acagatgtat ttaaaagatt
    19321 tcaaaatgtt tctaatctat ttaatataat tcaggattat gaattctgaa tataaacatt
    19381 catgaatatt ataaatgcat atcaataagt tatgagttga gcagaaagat agggggtatc
    19441 aatgattgtt gcgctaaaca cctggtgtga tcacgcgcac attaatgaag gcataaaaaa
    19501 aagccggcat catgccggcc caaaaaatat taacaagacg ataaaaggct aataacgtaa
    19561 gcatcataac acagaataat aaaaaggtat aattgtaatc agtgctaata tattgattaa
    19621 atgaattaat aaagccgata tcaaagataa ataaaatcgc tcaaaacgat ttttcctgta
    19681 ttcactgccg ttgcgcaaaa attatctatt tgttcaaaaa atgagtgagt cttgactggc
    19741 tcatccaatg tggaaaaatg tgacttttat cacataatag tactaagtct gagttttccg
    19801 ggttatctca aaatggaata cggttcgaca aagatggaag agagactctc tcgcagccct
    19861 ggcggcaaac tggctttgtg ggctttctat acatggtgtg gctatttcgt ctgggcaatg
    19921 gcgcgttaca tctgggtgat gagccggatc cccgatgccc ccgtgtcggg atttgaaagc
    19981 gatttaggct ctaccgcggg aaagtggtta ggggcactgg tcggattttt atttatggct
    20041 ctggtcgggg cattgttagg cagcattgcc tggtacaccc gacctcgtcc cgcacgtagc
    20101 agacgctatg aataaaaact gctaaggtgg cgactctttg gcaacggagc gcgtatggac
    20161 ttacaacact ggcaggcaca gttcgaaaac tggttgaaaa accatcatca gcaccaggac
    20221 gcggcccatg acgtgtgtca ttttcgccgc gtctgggcta cggcacagaa gctggcggca
    20281 gatgacgatg tcgatatgct ggtgatttta accgcctgtt attttcacga tattgtgagc
    20341 ctggcgaaaa atcatccgca aaggcagcgt tcttcaatcc tggcggcaga agagacgcgt
    20401 cgcctgctcc gtgaagagtt cgagcaattt ccggcggaga aaatcgaggc cgtttgtcat
    20461 gccattgccg ctcacagttt cagcgcgcaa atcgccccct taacaacgga agctaaaatt
    20521 gtccaggatg cggatcggct ggaagccctg ggggcgattg gcctggcgcg cgtgtttgcc
    20581 gtttcagggg cattgggcgt ggcgctgttt gatggtgaag atccgttcgc acagcatcgc
    20641 ccgcttgatg ataaacgcta cgcgctggat catttccaga ctaagttgct gaagctaccg
    20701 caaaccatgc aaaccgcaag aggcaagcag ctggcgcagc acaacgcgca ttttttagtc
    20761 gagtttatgg cgaagctcag tgccgaactg gcgggggaga atgaaggtgt cgatcacaag
    20821 gtgatagatg cgttttcatc cgctggctga gcgcgtggcc ctaaatggct gtaattatgt
    20881 taacctgtcg gccatctcag atggccggtg aaatctatgc aggaaaatat atcagtaacc
    20941 gattcataca gcaccgggaa tgccgcacag gcaatgctgg agaaactgct gcaaatttat
    21001 gatgttaaaa cgttggtggc gcagcttaat ggtgtaggtg agaatcactg gagcgcggca
    21061 attttaaaac gtgcgctggc gaatgactcg gcatggcacc gtttaagtga gaaagagttc
    21121 gcccatctgc aaacgttatt acccaaacca ccggcacatc atccgcatta tgcgtttcgc
    21181 tttatcgatc tattcgccgg aattggcggc atccgtcgcg gttttgaatc gattggcgga
    21241 cagtgcgtgt ttaccagcga atggaacaaa catgcggtac gcacttataa agccaaccat
    21301 tattgcgatc cggcgacgca tcattttaat gaagatatcc gcgacatcac cctcagccat
    21361 aaagaaggcg tgagtgatga ggcggcggcg gaacatattc gtcaacacat tcctgaacac
    21421 gatgttttac tggccggttt cccttgtcag ccattttcgc tggctggcgt atcgaaaaag
    21481 aactcgctcg ggcgggcgca cggttttgcc tgcgataccc agggcacgct gttttttgat
    21541 gtggtacgca ttatcgacgc gcgtcgtccg gcgatgtttg tgctcgaaaa cgtcaaaaac
    21601 ctgaaaagtc acgaccaggg taaaacgttc cgcatcatca tgcagacgct ggacgaactg
    21661 ggctatgacg tggctgatgc agaagataat gggccagacg atccgaaaat catcgacggc
    21721 aaacattttc tgccgcagca ccgtgaacgc atcgtgctgg tgggttttcg tcgcgatctg
    21781 aatctgaaag ccgattttac cctgcgtgat atcagcgaat gtttccctgc gcagcgagtg
    21841 acgctggcgc agctgttgga cccgatggtc gaggcgaaat atatcctgac gccggtgctg
    21901 tggaagtacc tctatcgata tgcgaaaaaa catcaggcgc gcggtaacgg cttcggttat
    21961 ggaatggttt atccgaacaa tccgcaaagc gtcacgcgta cgctgtctgc gcgttattac
    22021 aaagatggcg cggaaatttt aatcgatcgc ggctgggata tggccaaagg tgagaaagac
    22081 tttgacgatc cgcagaatca gcaacatcgt ccacgtcggt taacgcctcg ggaatgcgcg
    22141 cgcttaatgg gttttgaagc gccgggagaa gcgaaattcc gcattccggt ttcggacact
    22201 caggcctatc gccagttcgg taactcggtg gtcgtgccgg tctttgccgc ggtggcaaaa
    22261 ctgcttgagc caaaaatcaa acaggcggtg gcgttgcgtc agcaagaggc acaacatggc
    22321 cgacgttcac tataaggcca ctcgcagcaa aaatatgcgc gcgattgcca cgcgtgatac
    22381 ggcgatagag aagcgcctcg ccagtctgtt aaccgggcag ggcctggcat ttcgcgttca
    22441 ggacgccagt ctgcccggac gtccggattt tgtcgttgat gaatatcgtt gcgtgatatt
    22501 tacccacggc tgcttctggc accatcatca ctgctatctg tttaaagtgc ctgcgactcg
    22561 aaccgagttc tggctggaga agataagtaa aaatgttgaa cgcgatcgcc gcgatatcag
    22621 tcgtttgcag gaactcggct ggcgcgtatt gattgtctgg gagtgtgcat tacgtgggcg
    22681 cgagaagctg acggatgaag cgcttaccga gcgtctggaa gagtggatct gcggcgaagg
    22741 tgccagcgcg cagatcgaca cgcaggggat tcatttactc gcttgatgcg tcctgaataa
    22801 ctggcgcaac tacgggtttt gccgggaaga gatattttcc caacgtgacc agtaccaccg
    22861 cgaagacaat tacgccgagc gccagccatt caatcttcga cagcgtttct ccgcccagtc
    22921 ccgtacccag caagaccgcg accaccgggt taacgtaggc gtagctggtg gcgagagccg
    22981 gactgacgtt gcggattaaa tacatataag cgttaatggc gataatcgaa ccaaacagcg
    23041 ccagatagcc gaccgcaagg aagcctgaaa gggaagggag cgccgtcagt ttttcacctg
    23101 caatcatcga cgcgatcatt aacaccacgc ctgccgccag catctcaatc gcacccgcca
    23161 tcatccctac aggtaaggta atgcgcgagc cataaactga gccaaacgcc cagctaatcg
    23221 agccgattaa aatcagaatc gcgccccacg gattgccgct taaatttcca ccgctattga
    23281 gcatgatgat tccggcaagc ccaatggcaa tacccaccca ttccagtttg cgcgttttaa
    23341 tgccaaacag gcggctgaag cacagggtaa agaggggtac ggttgcaacc actacggcgg
    23401 cgatgccgga aggaacattt tgatgttcgg caaccgtcac catgccatta ccgacagcca
    23461 gcaataacag gccaatcagc gcggcattga gcagcggacg tagcgggggg agtttgtgtc
    23521 cgcgcagtag caaaaatgcc agcaataaaa taccggctgc caggaatcga acgcccgcca
    23581 tcattaacgg aggccagctt tccacgccaa tccgaatgac aaaataggtt gagccccaaa
    23641 tgatatacaa cgcaaacagc gcgccaaaaa gcggtaacaa ctggcggaaa cgcataatcc
    23701 ctcacggtgg aaataaaaag gtggttcata gtaaacgtga aaatcattct gctggcgaga
    23761 gatataattg cacttgattg ttaaaaaaat gttgacctgt gaagcaagta tcagagctgc
    23821 cgtttttgct tcatacttac acccttcaac aataaaaatg agagggaatg cttttggccg
    23881 ggagtagttt actgacgttg ctcgatgata tcgccacact gctggacgat atctccgtga
    23941 tgggcaaact ggcggcgaag aaaaccgccg gtgtattagg ggatgactta tcgctcaatg
    24001 cgcaacaagt ttcaggcgtg cgggccaacc gggaacttcc cgtggtctgg ggcgtggcga
    24061 aaggatcgct gattaataaa gtgattctgg tgccgctggc gctgatcatc agtgcgttta
    24121 tcccgtgggc gattacgcct ctgttgatga ttggtggcgc gtttctctgc tttgaaggag
    24181 tagagaaagt gctgcatatg ctggaggcgc gtaaacataa agaagatccg gcgcagagcc
    24241 agcagcgtct ggagaagctg gcggcgcagg atccgctgaa gtttgaaaag gacaaaataa
    24301 aaggggcgat tcgtaccgat tttatattgt ctgccgaaat cgtcgccatc acgctgggga
    24361 ttgtggccga agcgccgttg cttaatcagg tgctggtgct ttcaggcatc gcgctggtag
    24421 tgaccgtggg cgtctacggt ctggtagggg ttatcgttaa gattgatgac ctggggtatt
    24481 ggctggcgga aaaatccagc gcgctgatgc aggcattagg taaagggtta ttgatcattg
    24541 cgccctggtt gatgaaggcg ttatcgattg tcggcacgct ggcgatgttc ctcgtcggcg
    24601 gcgggattgt ggtacatggt attgcgccgc tgcatcacgc cattgaacat ttcgccgggc
    24661 agcaaagtgc agtggtggcg atgatattac cgactgtttt aaatctgatt cttggattta
    24721 tcatcggcgg catcgtggtg ctgggagtga aagccgtagc gaaaatacgc ggtcaggcac
    24781 attaatactc gcacaggcta ttatgcaaaa ttcgccatct tcaactaagg tgaacaagtt
    24841 tcactcgaaa aaaggaggcg agtatgagct ttatggttag tgaggaagtt acggttaaag
    24901 agggcggccc gcggatgatt gtcaccggat actccagcgg tatggttgag tgtcgttggt
    24961 atgacggtta cggggtcaag cgggaagctt ttcatgaaac cgagcttgtt ccgggggagg
    25021 ggagtcgttc tgcggaagaa gtttgaaagg gtatcattaa cacccgaccg gatgtggcgc
    25081 tcacgtcgca tccggtcggg tgtgccagtg tctgcactga attgcctgat gcgacgctat
    25141 cgcgtcttat catgcctacg ttataaaact atcgatgtct gtcgcgtctt atcatgccac
    25201 cattataaaa ttatcgctgt tttgcggtac gctatcgcca ggttatgcca gaatcataaa
    25261 aaagcaggtt gggagtcgtc agggtgcagc acgagacaaa aatggaaaac cagagctggt
    25321 tgaaaaaact cgcacgccgc ctggggcctg gtcatgtcgt taatctctgc tttatcgtgg
    25381 tattgctttt ttccaccttg ctcacctggc gtgaagtagt ggtgctggaa gatgcctata
    25441 tctccagcca gcgtaatcat ctggaaaacg tagccaacgc gctcgataag catttgcagt
    25501 ataacgtcga caaactgatc tttttgcgta atggcatgcg cgaagctctc gtagcgccac
    25561 tggatttcac ttcactgcgt aatgctgtaa ccgagttcga acagcatcgc gacgagcacg
    25621 cctagcaaat tgaactcaac cgacgacgca ccctgtcagt caatggcgta tcggatgcat
    25681 tagtcagcga ggggaatctc ctgtctcgcg aaaatgaaag cctcgacaat gaaattaccg
    25741 ctgcactgga agttggttac ttgctgcgac tggcgcacaa cacctcgtcg atggttgaac
    25801 aggcgatgta tgtctcgcgt gccggatttt acgtttcgac gcagccgacc ttgtttacgc
    25861 gcaatgtacc aacgcgttat tacggctatg tcacccaacc ctggtttatc ggccattcgc
    25921 aacgagaaaa tcgtcaccgc gcggtacgct ggtttacttc gcaaccggaa cacgccagca
    25981 atactgaacc gcaggttacc gtcagtgttc cagtagacag taataactac tggtatggcg
    26041 tgctggggat gagtattccc gtgcgtacca tgcagcaatt tttaagaaac gccatcgata
    26101 aaaacctcga tggtgagtat cagctctatg acagtaagct gagatttttg acctcttcca
    26161 atcctgacca tccaacaggg aatatttttg atcctcgtga actggccttg ctggcgcagg
    26221 cgatggaaca tgacacgcgg ggcggcattc gtatggacag tcgctatgtt agctgggaac
    26281 gtctggacca tttcgacggt gtgctggtgc gcgtccatac gctaagcgaa ggcgtgcgcg
    26341 gcgatttcgg cagtatcagc attgcattaa ccctgctgtg ggcgctcttt accaccatgt
    26401 tactcatctc ctggtatgtg attcgccgga tggttagcaa catgtatgtt ctgcaaagct
    26461 cgttgcagtg gcaggcgtgg cacgacacct taacccgttt atataatcgt ggcgcactgt
    26521 ttgaaaaagc ccgtccgctc gcgaaattgt gtcagacgca ccaacatcct ttttctgtca
    26581 ttcaggtcga ccttgaccat tttaaagcga ttaatgaccg ctttggtcat caggcgggcg
    26641 accgtgttct ttctcatgct gccggattaa ttagcagttc cttgcgtgcg caggacgttg
    26701 ccgggcgggt cggtggtgag gagttttgtg tgattctgcc aggcgcgagt ctgacggagg
    26761 ctgcggcagt cgcagaacgt attcgcctga agttgaatga aaaagagatg ttgatcgcta
    26821 agagtacgac gatacgcatc agtgcctcgc tgggggtaag cagcagcgag gaaaccggtg
    26881 attatgattt tgaacaactc cagtcactgg ctgaccgtcg gctttatctc gctaaacagg
    26941 ccgggcgtaa tcgggtatgc gcgagcgata acgcttaacg ggcggagaaa aaatggtcca
    27001 gcccttcacg ccagccttcc ggtccttcac gctgcgttcg ccagacgcgg gccggatcct
    27061 catcatgcag atgcacccct tcacggttta gccctttcac aatcaccgcg taatccatta
    27121 cctccagtaa gggcgcatcg tttggcccat cgcccaggcc aagtgtggtt gggcgtttgc
    27181 ctgacaattg ttgataagtc gcgataatcc agttggcagc ctgatctttt ccggcagagg
    27241 catccagtac atgccagaag cgcgcaccct gcataaactg taagcccagt tcgttcagac
    27301 gcgcggtaaa ttgtgccata cgctcgtcac tgtcgcgcca gattagcgtt accgacgcct
    27361 catgaagctg cgtcagcgcc gcctggctac ggcttaatcc cgtccattcg gcgatggttg
    27421 catcgtcgac atcatcaaaa gttgtgaatt taaaatgttc tttctcgcgt agcgtattta
    27481 aaacctggct gatttcgcca tggctaatac ctgagatgat gcgtggaaaa ccgtctatct
    27541 cctgccattg ttcagcaagc tggatcactg cgccattctc tgcaatcagc ggtaagcctt
    27601 gtagccccaa cattttttgc aagtacagca tttctgctga agttttacta ctacacagaa
    27661 tgacgggtac atttgcttcg cgtaaacggg tgagccaggg ggctgccggt tgccagtcat
    27721 aactatgact atccagcagg gtgccatcaa gatcgctaaa aaccagtagt ggttgttgaa
    27781 ttgaaaacat agtcgcgcag tactcccctt accaggatga ttctcaaaac agcacaaatt
    27841 atcaaagatg attttttcgg gagcgatccc gctgacgcca gagccaccaa ggggtgacgt
    27901 gcgtcacatt tctattcata agtagcgtta atcattcata tggcgaatat tttcttgtca
    27961 gcgaaaaaaa ttgcggataa ggtgatgaac acatcagatt tcctggtgta acgaattttt
    28021 taagtgcttc ttgcttaagc aagtttcatc ccgaccccct cagggtcggg atttttttat
    28081 tgtgcattca acgattcact tcagagacgc tgaagtcatg aatatccagt tcgaaggcat
    28141 ccgccaattc acgccatgta tgatattcac gtccatcgac gctaacgtgt tcagaatcgt
    28201 tctggctgcg atgaacttcg ctttcgctaa tttcattgat cgccgccagc agggcatcga
    28261 catcgacgct gacctcacgt tttgcggtat cgctgtactc ttttgcggtt ttcatcatct
    28321 ttcctcgcaa ccgttactct gttgttgccg ccagtcgtta agtccgcctg gcatctctta
    28381 agtatagacc gtcgagaaaa tcagcactcg cgcgcggctg gcgctgccag aaaaacgaaa
    28441 ttgttctaca ctggcacaaa gccacaggag gaaaacgatg aaggtgaatg atcgggtaac
    28501 agtcaaaacg gatggcggtc cgcgtcgtcc tggcgtggta ctggcagttg aggagtttag
    28561 tgaaggcaca atgtacctgg tttcgctgga agactacccg ctcggcatct ggttctttaa
    28621 tgaagcaggg catcaggacg gtatctttgt ggagaaagca gagtaatctt attcagcctg
    28681 actggtggga aaccaccagt cagaatgtgt tagcgcatgt tgacaaaaat accattagtc
    28741 acattatccg tcagtcggac gacatggtag ataacctgtt tattatgcgt tttgatctta
    28801 cgtttaatat tacctttatg cgatgaaacg gtcttggctt tgatattcat ttggtcagag
    28861 atttgaatgg ttccctgacc tgccatccac attcgcaaca tactcgattc ggttcggctc
    28921 aatgataacg tcggcatatt taaaaacgag gttatcgttg tctctttttt cagaatatcg
    28981 ccaaggatat cgtcgagaga ttccggttta atcgatttag aactgatcaa taaatttttt
    29041 ctgaccaata gatattcatc aaaatgaaca ttggcaattg ccataaaaac gataaataac
    29101 gtattgggat gttgattaat gatgtgctga ctgttagaag catcatggat gaaacagtcc
    29161 tcattaataa acaccactga agggcgctgt gaatcacaag ctatggcaag gtcatcaacg
    29221 gtttcaatgt cgttgatttc tcttttttta acccctctac tcaacagata cccggttaaa
    29281 cctagtcggg tgtaactaca taaatccata ataatcattg acatggcata ccctcactca
    29341 atgcgtaacg ataattcccc ttacctgaat atttcatcat gactaaacgg aacaacatgg
    29401 gtcacctaat gcgccactct cgcgattttt caggcggact tactatcccg taaagtgttg
    29461 tataatttgc ctggaattgt cttaaagtaa agtaaatgtt gcgatatgtg agtgagctta
    29521 aaacaaatat ttcgccgcag gagtatcctg gaagatgttc gtaggtatta tttttcggat
    29581 aatccttagg ataacatgat aaacgttacg gaattatatt aatggcaatt cactaataat
    29641 atcagccagc aaattaaaaa tttcactgaa taaatgttcg caaaaagacg caattaacgg
    29701 cattaatgcc gccattaaag agataccgac agtcagagtt aatggaaatc caataacaaa
    29761 aatggataat tgcggggcca tacgattcag taaacctaat gccagattca gggtcagcag
    29821 cagagtaatg agcggtaacg ccagcatcag cccgttaagg aaaatcaaac tccctgcttt
    29881 ggtgagtgcc agaaacgcat tgctgttcaa cggttcgcca ccaattggca gggtgtgaaa
    29941 ggtatcgacc agcagtgaaa ttaaccataa atgaccgtta aatgtcagga acagcaataa
    30001 cgccagcata tccatgatac gcgctaaaac gggc
//
LOCUS       DACAPB010000037        29697 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.037, whole genome shotgun sequence.
ACCESSION   DACAPB010000037 DACAPB010000000
VERSION     DACAPB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.037"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..99)
                     /locus_tag="IT904_001496"
     CDS             complement(<1..99)
                     /locus_tag="IT904_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP3191296.1"
                     /translation="MAELQKVDDWLSALLANLEPVARSRMMRQLAQE"
     gene            complement(92..523)
                     /locus_tag="IT904_001497"
     CDS             complement(92..523)
                     /locus_tag="IT904_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3191297.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAVAEPDEPEEMWTVKRG"
     gene            complement(619..1047)
                     /locus_tag="IT904_001498"
     CDS             complement(619..1047)
                     /locus_tag="IT904_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HAP3191298.1"
                     /translation="MNRLLLVVLVLLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSALLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGTAYRQWLSASDAVSAGSGNAAH"
     gene            complement(1044..1421)
                     /locus_tag="IT904_001499"
     CDS             complement(1044..1421)
                     /locus_tag="IT904_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191299.1"
                     /translation="MKKKLISGLFLMLWMALLIEAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALAALIKNGPLWQELRASLLKTITRKENVFISWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(1423..1935)
                     /locus_tag="IT904_001500"
     CDS             complement(1423..1935)
                     /locus_tag="IT904_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAP3191300.1"
                     /translation="MNPSIVKRCLVGTVLAIAALLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDAVVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(1916..2131)
                     /locus_tag="IT904_001501"
     CDS             complement(1916..2131)
                     /locus_tag="IT904_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191301.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2135..2338)
                     /locus_tag="IT904_001502"
     CDS             complement(2135..2338)
                     /locus_tag="IT904_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3191302.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2338..2802)
                     /locus_tag="IT904_001503"
     CDS             complement(2338..2802)
                     /locus_tag="IT904_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAP3191303.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLEGKSERIHHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(2898..3548)
                     /locus_tag="IT904_001504"
     CDS             complement(2898..3548)
                     /locus_tag="IT904_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HAP3191304.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3552..4610)
                     /locus_tag="IT904_001505"
     CDS             complement(3552..4610)
                     /locus_tag="IT904_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HAP3191305.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAEAGA"
     gene            complement(4627..5460)
                     /locus_tag="IT904_001506"
     CDS             complement(4627..5460)
                     /locus_tag="IT904_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAP3191306.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETVLQAQQAAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5603..7369
                     /locus_tag="IT904_001507"
     CDS             5603..7369
                     /locus_tag="IT904_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HAP3191307.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHNNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7369..8418
                     /locus_tag="IT904_001508"
     CDS             7369..8418
                     /locus_tag="IT904_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP3191308.1"
                     /translation="MGKSKKNRAAATNQLKHKSQTSAEAFSFGDPIPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASRVFVRNELMPLQKRLQEFNEWLGINII
                     HFEKYNLINDDPQST"
     gene            complement(8457..10649)
                     /locus_tag="IT904_001509"
     CDS             complement(8457..10649)
                     /locus_tag="IT904_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAP3191309.1"
                     /translation="MNDKNMLLGYGETLTNPVKLNRGGGEKNKPYSYSENKPVISAQL
                     EELIEEINTLPLLAMPEGKAVAKFVLHPAFLAKSYFPVSLFERFSLESIGSKAVKVKP
                     RKDIKKRGRKEEYTTACIYVSGKKESFQSFLDSVNQDTLTKGQQNDFVTLENISILEV
                     SDKVKTINSNEVMSIEVALHTPDTSSSIVDSFEVFASQNGAVIDKARSIKVKGLTFMP
                     IKASKDVALKVAEFSFLRTLRELPELRLSEPVISRSVIQTSNLSLPSEGAVNPHIKVA
                     IFDGGLGIDDFNPWVTEYTFNGNASTNAKLLSHGQDVTSTVLFGVLGSETEKLSVPYC
                     NIDHYRVLDSNVNNSDVDLFDVLIRIKSVLEQKKYDYINLSLGPRLPVDDDDVHVWTS
                     TLEEILASGETLCTVAVGNDGQLPAKLNRIQPPADLVNGLSVGAATSLSDSWERCSYS
                     CIGPGRSPGFVKPDGVAFGGHSDEPFQVYSPMVNGLARTAGTSFSAPLVLRQAIALSA
                     SLNYNITPLTAKALLIHHAESNNINRAEVGWGRFPHDLSEVIFCDDDEVKVIYQGTLK
                     PSQHMRAPIPFPDVPMRGCVNLRATFCFSSPVDAEHPLNYTRSGLEVTMRKGVSDSSG
                     LTFPLFNLKNVYADENEQRVDAHKWETTLRSEHKFKPNELTNPCFDIIYYGRDCGMPI
                     DVDELEELPYVLVVTLSAEEMPDLYNLIRQKYQTLQPIQVQQQIMLRT"
     gene            complement(10642..11724)
                     /locus_tag="IT904_001510"
     CDS             complement(10642..11724)
                     /locus_tag="IT904_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP3191310.1"
                     /translation="MINVTNEMLIDVVSDALTGKRESLLMRLRIMAKKLKKESPELAG
                     KLEDLLINTGGAIAVERANPVVPKITPVDADTRQKLLVETYPVVIDVEPVWPTKTDVQ
                     LNRFVAEWEMKERLYKEGLHPSKSLLMGGPPGVGKTLAAKWLAFKLDMPLLTLDLASV
                     MSSYLGKTGNNIKAVLNYASSFPCVLLLDEFDAIAKKRDDATDVGELKRLVTVLLQAI
                     DEWPNTSVLIAATNHAELLDPAAWRRFDRVIDFEYPTQDLMRRYLCSKNIGDSLSYYI
                     ATKLEKVSYAVLERAINQAKRNAIIEDIPLSSALIDELLDGVSLDDVIKNMSNNNVSQ
                     RKIALDLGISRSQVRKVLTVEDEDNE"
     gene            complement(12140..12373)
                     /locus_tag="IT904_001511"
     CDS             complement(12140..12373)
                     /locus_tag="IT904_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAP3191311.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLHPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(12384..12572)
                     /locus_tag="IT904_001512"
     CDS             complement(12384..12572)
                     /locus_tag="IT904_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191312.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(12725..15139)
                     /locus_tag="IT904_001513"
     CDS             complement(12725..15139)
                     /locus_tag="IT904_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HAP3191313.1"
                     /translation="MSHADMNNCSGVNEVAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYATDNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRKTIKPCLERLEHVRD
                     SQLSTSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAKMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTEPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLCDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQVAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQQGKDKSADGDTRTDITRMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     KWRDLY"
     gene            complement(15136..15993)
                     /locus_tag="IT904_001514"
     CDS             complement(15136..15993)
                     /locus_tag="IT904_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HAP3191314.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETEY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVD
                     SSAVYGVRA"
     gene            complement(15990..16217)
                     /locus_tag="IT904_001515"
     CDS             complement(15990..16217)
                     /locus_tag="IT904_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HAP3191315.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEVPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(16217..16450)
                     /locus_tag="IT904_001516"
     CDS             complement(16217..16450)
                     /locus_tag="IT904_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HAP3191316.1"
                     /translation="MRNIETLTTKTGPDDVGLNILLTEARLEERRARAEAMAARLDSL
                     ACHITSHQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(16518..16859)
                     /locus_tag="IT904_001517"
     CDS             complement(16518..16859)
                     /locus_tag="IT904_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HAP3191317.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINSEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            complement(16823..17023)
                     /locus_tag="IT904_001518"
     CDS             complement(16823..17023)
                     /locus_tag="IT904_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HAP3191318.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            complement(17031..17540)
                     /locus_tag="IT904_001519"
     CDS             complement(17031..17540)
                     /locus_tag="IT904_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HAP3191319.1"
                     /translation="MFDYKISKHPHFDEACRAFALRHNMAKLAERAGMNVQTLRNKLN
                     PDQPHQLNAPEIWLLTDLTEDSTLIDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            complement(17573..17815)
                     /locus_tag="IT904_001520"
     CDS             complement(17573..17815)
                     /locus_tag="IT904_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP3191320.1"
                     /translation="MSTDISIRVPKEMATPAEFAEWEGISRGSVYQKIHHGQLAKYMV
                     KKEKNKGRVSLRYLMYKTDQVRESLGHSNFRVIVGK"
     gene            17935..18567
                     /locus_tag="IT904_001521"
     CDS             17935..18567
                     /locus_tag="IT904_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HAP3191321.1"
                     /translation="MIQVKAGENTGGREAIHRLMAAYDFKSRQQLCDHLGASKSTMAN
                     RYLRDSFPAEWVIQCALETGVSLLWLTTGQGEPGPNIEPKKNINSVNSSKVVPLSELV
                     SPEIDKATLNGGLLVDAGKAIIDSSILPSDSSNLLLVTTSGDSYLIDRNQTPPVNGMW
                     LVDIDGIKSIVKLTRLPGNKLVVHQDDSSFECGLDDIEVVGRALKIIKSL"
     gene            18569..19582
                     /locus_tag="IT904_001522"
     CDS             18569..19582
                     /locus_tag="IT904_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP3191322.1"
                     /translation="MTIRKQPNGKWLCECYPNGRNGKRVRKQFATKGEAIAFESFTME
                     EVNKKPWLGEKDDRRRLSELIELWYSLYGQTLADPKRLMAKLRIICNGLGDPIASELT
                     AGDFTKYREARLKGEIRNEDGTLMSPVKPRTVNLEQRNLSSVFGTLKKLGHWSAPNPL
                     AGLPTFKIAEGELAFLSTDEIKRLLAACAESQNPSLLMIAKICLATGARWSEAENLQG
                     HQISKYRITYTKTKGKKNRTVPISQELYDELPKNRGKLFSPCRKAFERAVKRAGIDLP
                     DGQCTHVLRHTFASHFMMNGGNILVLRDILGHSDIKMTMVYSHFSPDHLEDAVTKNPL
                     NLI"
     gene            complement(19596..21353)
                     /locus_tag="IT904_001523"
     CDS             complement(19596..21353)
                     /locus_tag="IT904_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HAP3191323.1"
                     /translation="MREHKFISELFLENSQFILIGLTGRTGSGCTTAANILEQKEISF
                     PDSNVLAEYYKGLDLRRYEIVKNFAETHWKNFYSIKVSDLISIYLLLLSPSALAKFIQ
                     FSASKIPDKKENSISFKAAKKLVVNGVFAKSLLKIKYLEVIKNLLNHDDEIDFKGRGK
                     DTFIRILTLTRKFTRDFKRELNELEPGLYIELYQSAGKSIRRIGKVDVDYENQDFIPK
                     AVFHLPETINRVIKIIRKVKNNKAYIVIDAIRNPYEAKFFKDRYAAFHLVSINAPDEH
                     RTRYLKKLHKFSEEQIKKINDEESGKGDGEYNHLTNPNVKKCIEISDIHFFNPKKEFD
                     NNNFLKAQIAWYVSLMQHPGLISPTAMERVMQVAYTVKLNSGCISRQVGAVVTDNDNS
                     IKSVGWNDVAKGQVPCSMRSFDGLLHDFDEGTYSLYERSNTKFRSKVKENLIKIRASD
                     SSSTVFKGLNLPYCFKDIHNSLDDEKKGNQVHTRALHAEENAFLQLAKYGGVGINGGK
                     LYTTASPCELCAKKAYQLGMTEIIFIDPYPGIAQEHIINIGSFSPKLIQFRGAIGKSY
                     HRLYEQVIPMKDELDYLMK"
     gene            complement(21781..26359)
                     /gene="ypjA"
                     /locus_tag="IT904_001524"
                     /pseudo
     CDS             complement(21781..26359)
                     /gene="ypjA"
                     /locus_tag="IT904_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
     gene            complement(26424..26543)
                     /locus_tag="IT904_001525"
     CDS             complement(26424..26543)
                     /locus_tag="IT904_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP3191324.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(26698..26916)
                     /locus_tag="IT904_001526"
     CDS             complement(26698..26916)
                     /locus_tag="IT904_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3191325.1"
                     /translation="MKKLVRTSSAGNALLVCKLNRLGRSMWHLVVLLEELCERGINFR
                     ALAQSIFAQQWGDECCKSKTICDLKVIV"
     gene            complement(27271..28644)
                     /locus_tag="IT904_001527"
     CDS             complement(27271..28644)
                     /locus_tag="IT904_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191326.1"
                     /translation="MLVSKSNGFNASAVLGSGSYNENKSSKHMELLAHSILKLICKEA
                     ASETYRGALETLQKMMSECIYQEGNAFVIMGAGEQLKRIKYEVGENNLKVFNVHFNNN
                     HELVSSGEPDVICLSKQVWENLLIKLKLENNENVFSETKKLSNKNNADQFFECAKRNE
                     WNLFDNIRKSDFHVGLLKPSSTRSVILETPPNVCMESRNSYENKIDEISSLSESKEHP
                     IDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECCYKFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTPCIFNAPAYNKSNEKSLKP
                     VSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVS
                     SSKSSFQ"
     gene            complement(29395..29470)
                     /locus_tag="IT904_001528"
     tRNA            complement(29395..29470)
                     /locus_tag="IT904_001528"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29435..29437),aa:Ile,seq:cat)
BASE COUNT         7630 a         7553 c         6434 g         8080 t
ORIGIN      
        1 ttcctgcgcc agctggcgca tcatgcggct tcttgcgact ggttccagat tcgccagtaa
       61 ggcactaagc cagtcgtcca ccttctgcag ttcagccacg tttcaccgtc cacatttctt
      121 caggttcatc gggttctgct acagcttcaa cgctcgacac actgccgtca gtgctgacca
      181 gcacacgttc cgtcagttgc aggttaaggc tgatatcaca gacatcgttg cgcagaatat
      241 ccacctcaaa ggtaaatagt ttttcccgta acgccgggtt attgatggca tcgggctggt
      301 tatcccgcag ccacagcaaa accggggcca tcagcagatt ctggtcgccg ctgaaatcct
      361 caatcaccgc gttcagggtg taacggtact cccatgacat ggagctggcc cccgtggcaa
      421 ccagcgaacc gttatccaca aacagatgca gtttgtccgg gttattgcgg acataaggca
      481 ccgctttatt gagggcgtgg cgcagggatt gtggtttgtt cactgtttcg ctcctgacac
      541 gcaataatca tgtccacttt gtctgcacag accgcccagg cggcctccgt ttcatccagc
      601 aacgcgttca gatcaccgtt agtgcgcggc gttgcctgat ccagccgaca cggcgtcact
      661 cgcggacaac cactgacggt aagctgtacc tccggtgagt gccggacgtt cccgcagccg
      721 gataatgtca gcaggcaaag gagtatcagc ccagcggcgt aaatcctcgt tctcacgttt
      781 cagttcctcg atccggtgtt gtcgttgtct cagcagtgcg ctggtctgtt ctgcttcggc
      841 atagagccgc gcctgctccc ggttattggt ttcagtcaga atggacaggc tgataagctg
      901 gctgttgctc tttgccagtg cctggctttt gctctgcagc tcgtctgcct gcgtgctgat
      961 ggtctggctg gcatcagcca gccgccacgt ctgccagccc agcgccgcca gtaataacac
     1021 cagcacaacc agcagcaacc tgttcatgct gctacctgtt gcgccatctg attacgggtg
     1081 atccagaagg caataacggt cagtagataa aagaccaggg taatagccca ccccgtccag
     1141 gcgagactta cgacaatcag caatcgcatc acccagctga taaatacgtt ttcttttcgg
     1201 gtaatggtct tcagcaaaga tgccctcaac tcctgccaga gcgggccatt cttaattaac
     1261 gcagccagtg ctaccggaat taccgcccat gtcagcagac aggctaccca aacgccggac
     1321 gctgccagta ccggaaaaat cccctgcgga tacaccattg cttcgattaa cagcgccatc
     1381 cataacatca gaaacagccc gctgattaat ttctttttca tttcagtttg ctccctgtaa
     1441 gcaccaggcc atctcccgcg cacggcggtt atccagcccc tgattaaaaa cacctttcac
     1501 ataaacccag cgcggcaact gtcggcacgc atccgcccag cgccgctgat tgagcaattt
     1561 caccagcgtg gaactgcagg cattgcccgt tcccacgttg aaggcaaacg acaccaccgc
     1621 gtcatacacc ttctgcggcg gctgttgctt cacacacctt tccagcgccc gctccacacg
     1681 cagcacgttg gagatcagcc cttccgctgc ctgtcgttcc gtaatggttt tgccgggaat
     1741 gacgcccgac gtattaccaa tgccgtcagt ccatacaccc gcactacact gatacggctg
     1801 tagacgacag ccttcgtaat cggcaatcag tttcagcccc tccacggagg tgtgaagctg
     1861 ctgaaaaccc ggcagcaagg cagcaatagc cagcacggtc ccgacaaggc agcgtttaac
     1921 gattgatgga ttcatagtcc tcccgcgaga tctgcccgtc gcgcagaagc tggtaggctt
     1981 tgtgtttgta gtaccagttg atagccagca tcagcacacc aatcatcagg ccgcccagcg
     2041 ttgaggcatc cttgatggac aaatcgccca gccaggccag cacgacggcg atgcaatacg
     2101 tgataaaagc gctgattcgc tcaagcgtca taattcagtc ccatagctgg acggtctgca
     2161 cggtggtggt tgtcggtatg tccggcagct ccacctgcag cccgtgaggt aaaaaggggc
     2221 cgtattcggc aagccccgga tttgccttca gtacctgctc cgtgacaccc tgcgtgcgcc
     2281 cgtaatgacg ccagcaaagt gcgtccaccg tgtcatactg atgcgcacgc actttcatca
     2341 gataagctcc actgtgcagt gcggcgcatc ctgtacccgg ctgatggccc agcgggcatc
     2401 acgccacaaa tcaccgcttg cttccgccag ttcctcgccc cgcttcacac cggatgccgt
     2461 ggcgtcatag tcctgataac gctcgttgag catggcgcgt gcccagcagt aaaccgcgtt
     2521 gaaatagtga tgaatgcgct cacttttgcc ctccagctgt tccgccggaa cctctgccag
     2581 cgaggcatat cccagcatct gctgacgtct gcgaaactca tacagctctg cgttgacctc
     2641 cgaaattgcc gacagcgcaa cctgctttaa acgcggctgc gtcactgtgc cgtcagtgcg
     2701 catgacactg cgaaactccg acaggtccac atcaggccag aacggcgtat ttctgatgat
     2761 ttccgcctgt tccggtgcct gttctggcgc aacaaacttc atgctgcttt ctcctgaaat
     2821 aaagggcggt ggacggggtt ttgatgtggc agtgcctttc gccaccccgt gccgcccgtg
     2881 cgcgggggca cgttctgtca gcggctgtca ttgcgcagtc tgcgctccag ctgctgtttg
     2941 tcttttttca cgccacagcg gggatcgagc tgtaacgcat ggttgagatg attaagggcg
     3001 gaagccggat tgctttcact caggacagcg ccaatcgctt tatgcagacg cgcccgtgac
     3061 tggtccggca tatccagacc gtctgtcagc tccagcgtct gcagcaacag atcggcatca
     3121 aagccggtgg cggcaagcat tgcgctctgc gctgcatctg ccatttcctc tgccagcacg
     3181 gtctgcacgt tgcggttacc cagcggcatc acccagccat gacgcagggc atgacgccct
     3241 atctccagcg ccccggcata atctccggca tcaatgcgcc acagcatcac gtacatcagc
     3301 acgtcatcct gttgagcgcc tccggcagcc aggacgccct ccgcccaggc ggcatatttc
     3361 ggcagcagct ccacctttat ttccgctttt ttcaccgtgg actgaacgcc cttgagacgg
     3421 cggcggtctt ccgccagctg cagcagcatc aggtcatagc ccgacgcgtg gcgaacactg
     3481 ccgccctcac gggcggcctg ttcagcctga acgcgcaggc gatgctgccg tgcgggactc
     3541 aggctcatgg tttacgctcc ggcttctgct gcggcggcgc tgaaatcacc aatctggatg
     3601 ttttccacca gtgcggcgca gcggtagtcc tcaaccacat aggcttcatt aacggattca
     3661 aagttttcaa tccggtcacg tttcgggttg tcgataactg aacggcggcg ggtgtcttcc
     3721 tgccagtaga tggacaagtt atccagacgg gtgatcagca gtgcattcgg cgggaagaac
     3781 ggcgcacgca cggcctgcag gccacccatg cgtttctgac tgatgatcat atcggcagcg
     3841 agtttttcac tgttttcctg ctctttgttg accagtggga aatacttgtc agacaacagt
     3901 tcacgaccgc aaatcaccac cagatcgtca tcgtcctggt agaccacgtc gataagctca
     3961 ttgacggcat ccatcaccac ggcgtccagg ttggcatatt cgccaccttt accgactttc
     4021 accgcgcccg gtgtggtttc accgcctgtg gtggtgctgc ccatgacgtg atccggtgca
     4081 tcctcacgga ttttctgcag ccagcctttg ttcacatcct gcagcagcgg gttttcacta
     4141 cggttggagg ttttcgcacg cttcacgccg ttaaagccga tcatgatgcg gtccagtgcc
     4201 tgacgtttca cgatggcgtc acggatacgc acctggaaat cctgaaattt cgcccacagg
     4261 tccagcttcg cgtaggtcag caccgtgtca aagttggtct gctcgcattt gtattccaca
     4321 tcgaccatca gcgtcggatc gacaggttca cgctctttcg cggtggtatc agtggttccg
     4381 gcaatggtgc tgccaacacc caaccccagc agctgaccgg actgctcagt cactggcgtg
     4441 acgttaatca gcgtcaggaa agcggcggac tgctggatct ggtcttccag cgtctgctgt
     4501 acagacggct ccacggtgaa cttgctggac agttcttcaa ctgccacacc gttcagacgc
     4561 gccagctgct gcaggtaagc gttaaaagca aagcgggtat tcttcttcat caggttttgt
     4621 gctccatcag caattggtca gagtgtcagc gggggcgtta ccgcccgttg cacgctggcg
     4681 gtagtcctgg cggctgtctt catggctcag cttgtccacc agttcgttaa aggcggcctg
     4741 ttgtgcctgc agaactgtct ccagctcaga cagacgttct tcctgctcag aaagggattt
     4801 ttcggtgcgc gcactcaggt tttgctgctc agtggcgacc agttccacgg ccttatgcac
     4861 atcagaaaac cgggcatcgt cggactgctc tttttttgta aacagcgccg tgacacgggc
     4921 aaacagggac ggtttgtcat cctggatttc ttccagttcg atcaccgttt cctctgcggc
     4981 ggtaaagaga ttggcgggat tctgcttgcg gtttgccagc gggttatggg ctgcgctggc
     5041 gctgaatgtc agcatttccg tacccagact ggctggatca tcagtggcag ccaggccgac
     5101 caggtaggct ttgcccgtat cagcaaactt cgggctgact tccatagagg tgaataattt
     5161 ctggcctttt ttcaccagtt ccaccaggga ctccgttggc tcaacgtcgg catacagtgc
     5221 catcttgccc gccagcggac cctccgtgat ttcttcagca aacagcgccg tcaccttgcc
     5281 gtagcggtta aaggtgctgt ccggcagata agacttgatg tgctcaaggt taatcagcgc
     5341 ggtatacacc gccgggttgt agctggctgc catctgttcc agccattcac gctggatttc
     5401 gcgtccgtcg gtggtggcac cttccacccc gatgcgaaaa cgctttgctt tcactgtcat
     5461 gagccgtgct ccgttagaaa taacttactg gagccttatg gttgcggtga tgggggcagt
     5521 gaaacaatgc gcggtatttg taccgacaac cacacaaacc gcaggcgggg aaagccttca
     5581 ttcaaggctg taggtttgtg ccatgaacac cacactgaca cccgcagatc tcgatccccg
     5641 tcggcaggcc atgctgctgt actttcaggg ataccgcgta gcccgcattg ctgaaatgct
     5701 gggcgagaaa gttgcaaccg ttcacagctg gaaaaaacgc gacaagtggg gtgactatgg
     5761 gccgctggat cagatgcagc tcaccaccgc cgcacgctac tgccagctca tcatgaagga
     5821 gcacaaagaa gggaaagatt tcaaagagat tgacctgctg gcgcgccagt ctgagcgcca
     5881 cgcgcggatc ggcaagttta acaatggcgg caacgaagcc gacttaaacc ctaacgtcgc
     5941 caaccgcaac aaaggcccgc gccgtcagcc ggaaaagaat gtcttcaccg atgaacagat
     6001 tgagaagctg gaagaaatct tccattcctc catgttcaac taccagcgcc actggtggga
     6061 agccggaaaa accaaccgca tccgcaacct gctgaagtca cgccagatcg gcgcgacctt
     6121 ttactttgcc cgtgaagccc tgattgacgc cctgcttacc ggacgtaacc agattttcct
     6181 ttccgccagt aaagcacagg cccacgtctt taagcagtac atcatcgact tcgccaaaga
     6241 agtcgaggtg gagctgaaag gcgatccgat ggtgcttcct aacggggcca cgctttactt
     6301 cctcggcacc aatgcccgca cggcccagag ttaccacggc aacctgtatc tggatgaata
     6361 tttctggata ccgaaattcc aggagctgcg caaagtggct tccggtatgg ctattcacaa
     6421 aaaatggcga caaacctatt tttccacgcc atccagcctg acacacagtg cttatccgtt
     6481 ctggtccggt gcgctgttta accgtgggcg caacaaagcc gataaggtgg acatcgacct
     6541 gtcccacaac aatctggccc ccggcttgct ttgcgcagac gggcaatacc gccagatagt
     6601 caccgtggaa gatgcggtgc gcggcggctg taacctgttc gacctcgacc agctacgcat
     6661 ggagtacagc ccggacgaat atcagaacct gctgatgtgc gagttcgtgg acgatctcgc
     6721 gtccgtgttt ccgctcagcg agctgcaggc gtgcatggtg gacagttggg aagtctggac
     6781 cgactttcat gcactggccc tgcgcccgtt tggctggcgc gaagtgtgga tcggttatga
     6841 cccggcaaaa ggtacacaga acggcgacag tgccggatgc gtggtggtgg ctccgccagc
     6901 cgtgccgggc ggcaagttcc gcattcttga gcgtcaccag tggcgcggaa tggactttcg
     6961 cgcccaggct gacgccatca aaaaactgac cgaacagtat aacgtgacct atatcggcat
     7021 cgactcgacc ggcgtcggtc acggggttta cgagaacgtg aaagcgtttt ttcctgccgt
     7081 ccgggagttt gtctacaacc ccaacgttaa aaacgccctg gtactcaagg cctacgacat
     7141 tatcagccac cgccgtctgg agtttgacgc cgggcacacc gacattgcgc agtcattcat
     7201 ggcaatccgt cgcgccacaa ccgccagcgg caaccgcccg acctatgaag ccagccgcag
     7261 cgaagaagcc agccacgccg atctggcctg ggcaacgatg cacgcactgt ttaacgaacc
     7321 gctgcagggc gaatccgcca ataccagcaa tattgtggag attttttgat gggaaagagt
     7381 aagaaaaacc gcgctgcggc gacgaatcag ctcaagcata aaagccaaac ttcagccgaa
     7441 gcattcagct tcggtgatcc cattccagta cttgaccgcc gcgaactgct ggactatgtg
     7501 gaatgcgtac agacagatcg ctggtatgag ccgccagtaa gctttgacgg actggcgcgc
     7561 accttccgcg ctgccgtgca tcacagttca ccaattgcgg tgaaatgcaa cattctgacc
     7621 agtacctaca tccctcaccc gctgctcagc cagcaggctt tttcacgttt tgtgcaggac
     7681 tatctggtat ttggtaacgc ctacctggag aaacgcacga accgcttcgg tgaagttatc
     7741 gcccttgagc ctgctctggc aaaatacacc cgacgcgggt tagacctgga tacctactgg
     7801 tttgtgcaat acggtatgac aacccagccg tatcagttca cgaaaggcag catttttcat
     7861 ctgatggaac cggatattaa tcaggagatc tacggcctgc ccggctatct ttctgccatt
     7921 ccatccgccc tgctcaacga atccgccacg ctgttccgcc gtaagtatta catcaacggt
     7981 agtcatgcag gcttcatcat gtacatgacc gacgccgcgc agaatcagga ggatgtgaac
     8041 aacctccgca acgcgatgaa aagcgccaaa ggtccaggca acttccgcaa tctgtttatg
     8101 tactcgccta acggcaaaaa ggacggactt cagattatcc cattgtcaga agtcgcggcg
     8161 aaggatgaat ttcttaatat caagaacgtg agtcgggatg acatgatggc agcgcaccgc
     8221 gtgccgcctc agatgatggg gattatgcct aataatgttg gggggtttgg ggatgtggag
     8281 aaggcgagtc gagtgtttgt tcgaaatgaa ttaatgcctt tacaaaaacg attacaggaa
     8341 tttaatgaat ggttaggaat taatattatc cattttgaaa aatataactt aataaacgat
     8401 gacccacaat caacatagat aaagcttaat aaaggagggg gaacccctcc acattgttaa
     8461 gtacgtaaca taatttgctg ttgaacttgg ataggctgta aggtttgata tttctgccta
     8521 attaggttat ataaatccgg catttcttca gcagataaag tcacgacaag aacatatggt
     8581 agttcttcta actcgtcaac atcaataggc ataccacagt ctctaccata gtagataata
     8641 tcaaagcatg ggtttgtcag ctcattcggt ttaaatttgt gttcacttct taacgtagtc
     8701 tcccatttat gtgcatcaac acgttgctca ttttcatctg cataaacatt ttttagatta
     8761 aaaagaggaa acgttaaccc ggaactatct gaaacacctt ttctcatggt tacttctaga
     8821 ccgcttcttg tataattcaa aggatgttcc gcatctacag gactggaaaa gcagaatgtc
     8881 gctcttaaat ttacacatcc acgcataggt acatctggaa acggaatagg tgctcgcatg
     8941 tgttgtgatg gttttaaagt accttgatat ataactttaa cttcatcatc atcacaaaat
     9001 attacttcac taagatcatg aggaaatcgt ccccaaccaa cttcagcacg attgatatta
     9061 ttgctctccg catgatgtat aagcagagct ttagccgtaa gtggtgtaat gttgtaattt
     9121 agagaagcac ttaacgcgat tgcttgcctc aggactaatg gagctgaaaa gctagtcccc
     9181 gcagtgcggg caagaccatt aaccataggg ctgtatacct ggaatggctc atcagaatgt
     9241 cctccaaatg caactccatc aggcttcaca aagccagggc ttcgtccagg accaatacaa
     9301 ctgtaagaac atctttccca actatcagat aaagaggttg ctgcacctac tgacagacca
     9361 ttaactaaat cagcaggtgg ttgaattcga tttaacttgg caggcagctg accatcattt
     9421 ccaaccgcga cagtacaaag tgtttctcct gatgcaagta tctcctcaag agttgaggtc
     9481 cacacatgca cgtcatcgtc atcaacaggt aatctaggtc ctaagctcag attgatataa
     9541 tcatattttt tttgttctaa tacactttta atacggatca gaacgtcaaa aagatcgaca
     9601 tctgagttat taacattgga atccagtaca cgataatggt caatattaca ataaggaaca
     9661 cttaattttt ccgtttcaga gccaagaaca ccaaataaca cagtagaggt gacatcctgt
     9721 ccatgagaaa gaagttttgc attagtacta gcattaccgt tgaaagtgta ctctgttacc
     9781 caaggattga aatcatcgat ccccaatcca ccatcgaata tggcaacctt tatatgtggg
     9841 tttaccgctc cttcagatgg taaactaagg ttagatgttt gaatcacaga acgtgatata
     9901 acaggctcac ttagccttaa ttcaggaagt tctctcaaag tcctgagaaa tgaaaactct
     9961 gcaactttta aagctacatc tttactagcc ttaataggca taaaggttag ccccttaacc
    10021 tttatacttc ttgccttatc tataacagca ccattttgcg aagcaaaaac ttcaaaacta
    10081 tcgacaatag aggaacttgt atcaggagta tgtaaggcga cctcaatact cataacttca
    10141 ttgctattaa tcgtttttac tttatctgaa acttccagta ttgaaatatt ttctaaggtg
    10201 acaaaatcat tttgctgccc tttcgttagg gtatcttgat ttacagagtc taagaatgat
    10261 tgaaaacttt cttttttgcc agaaacataa atacacgctg tagtgtattc ctcttttcgc
    10321 cctctttttt taatatcttt tctaggtttt acttttacag ccttactgcc tatgctttct
    10381 agggaaaatc gctcaaaaag acttactgga aaataacttt ttgctaagaa tgccggatgc
    10441 aaaacaaatt ttgctacagc cttaccttca ggcattgcta acaatggaag ggtattaatt
    10501 tcctcaatca actcttccag ttgagcagat atcactggtt tattttctga ataagagtaa
    10561 ggtttattct tttcacctcc acctctgtta agtttaacag ggttagttag tgtttcacca
    10621 tagccaagaa gcatattttt atcattcatt gtcttcatcc tcaaccgtca aaaccttacg
    10681 tacttgtgac cttgaaatcc ccaaatctaa agcgattttt ctttgtgata cattgttgtt
    10741 tgacatgttt tttataacat catcaagtga cacgccatct aacaactcat caattaaagc
    10801 tgaacttaaa ggaatatctt ctataatggc atttctttta gcttgattta tagctctctc
    10861 aagaactgca tatgaaactt tttcgagttt tgtagcaata tagtacgata agctatctcc
    10921 aatattttta gaacagagat atcttctcat taggtcttgt gttggatatt caaaatcaat
    10981 aactcgatcg aatcttctcc atgcagcagg atcaagcagc tccgcatggt tagttgctgc
    11041 tataagaaca gacgtatttg gccattcatc aattgcttgc aataatactg taactaatct
    11101 ttttagttct ccaacatctg ttgcatcatc ccttttttta gcaatcgcat caaattcgtc
    11161 aagaagcagt acacaaggga atgacgaagc ataattgagg actgccttaa tattattacc
    11221 cgttttcccc aagtagctac tcattacact tgcgagatct aatgttaaca atggcatatc
    11281 gagtttaaaa gcgagccact ttgcagccaa agtcttcccc acaccgggtg gtcctcccat
    11341 aagaagagat ttggacggat gcaacccttc tttataaagg cgttctttca tttcccactc
    11401 agccacaaat cgattcagtt gaacatcagt ttttgttggc caaacaggtt caacatcaat
    11461 gaccacagga tatgtttcaa ctagcagctt ttgtcttgta tccgcatcaa caggtgttat
    11521 tttaggaaca actggattag ccctttcgac tgcaattgcg ccacctgtat taataagtaa
    11581 atcttctaat ttacccgcta attcaggaga ttcttttttt agtttttttg ccataatccg
    11641 caaccgcatg aggagacttt ccctcttccc ggtaagagca tcactgacaa catcaatgag
    11701 catttcgttt gtaacgttta tcatctaacc acccttatgg caaaaccgaa ccacttttca
    11761 aacaatcata caccaaaaaa acctttttaa ggtaattttg aaccaaaaaa aaatattttt
    11821 agcaccacaa atgctcaaaa caaaaccagt gacacttttg tataacaact aaaactacct
    11881 atcgacaaaa atttagctaa accttttcag cgcgcgctcg tatccccgcc acgcctaccc
    11941 gctttatgta gtggttttca tgcacctgca tgatctacgc aaaagcccac cagttctggc
    12001 gggccttagc aaaaacgatc ctcaaacgat catgcgatct catgcggcat agacatgcac
    12061 tacagagcta acgcctcgca agggctcgtt gttcaacctt gctgacgcca gaagcaagtt
    12121 cagacgccag caacgtttct taatgcaacc agctgtcgtc ttcccacact ttctgcataa
    12181 ttttcatcac ttgttttctt tcttcgtcca gttgcagtcc ggtaagttcc acaccgttag
    12241 agctaccttt gcggatacga attaccgttt ttggatatag ggggtgcagg ttgcggtaaa
    12301 gctcggactc cagagcgtcc agggtagact ggctaatctt ctgctcttta tcgatcatta
    12361 tttcaatgcg cataaaagtc acctcagctg atgacatcca ttgagcggtt gtattcgtgg
    12421 gttctgattt ttgccatgag ttcatctgtt agttcagaaa cccactgcaa agccagcccc
    12481 ttctcttcat cactacactc actagccgct acaagcttaa gaaaaaaatc aatgcgctgg
    12541 agcttcaaag actccaaaaa atagtcctgc atctttcctc ctatgacacc aaagcaatac
    12601 tgtatacata accactgttt atatttacag tatataataa tcttactgat gtaaaacgtt
    12661 tttttacgtt catcagcctg atatgcctgg tattattaag agcacgaatt gttaacccgc
    12721 gtaattaata cagatcccgc cacttatcat cttcctgcaa acgctggttc cgatagaaga
    12781 tacgcaggcc tgctcctgac ggaatactgc cgccgcgaag gagtaaatcg acctctttct
    12841 cgctaccatc aaatcctctg gacttcagct catagacgag ctgctgtcgc tgatggtctg
    12901 taattcgctg tttgtagtct ttacgacgtt tcggtttcac caggcgtaac cttgcagcca
    12961 gttcccggcg ctcttttttg ctcatactgt gcaggtaatc gtgcaactcc ttgtcatcca
    13021 tgcgggtaat gtccgttctg gtatccccat cagctgattt gtctttccct tgttggttca
    13081 aattttcagc aaggggacag ttattgccac gagtccaagg ggcgcaagcg ccctggtcgg
    13141 ctgccgcctc ctgaacgtca acggctttac gaaccatttt ccacttcact gcatgagtgc
    13201 agatcttgcc ctctgcaatg ggtgaccaga tgccataaat acgaataccg tgatcgccat
    13261 aggcggtcgg ctcttcgttg atttcataag cggttctgat gaggtgatat ttacggggaa
    13321 ccagtacgcc gccctgcttc atgatgtagg tagcaaaaca accagcatca gcagcagcca
    13381 gaatggcatc aagacgcggg ttatccagta ccggcgcacc tgcttttttg tcaccctgtt
    13441 gccttgccac ctgaccagcc agcaagcgaa gttcacggta agcctgacgt cccggaatac
    13501 caaagaagcg gaattgctgt acacgatgca gagacgccca ggcattaacg tattcagcgt
    13561 tatcacacaa ggatttaccc gtttccttac tgatctcgcc agccagacca cgcccgtcaa
    13621 tgttcttact gatgtatttc gcgatgtagc ttgtcggcgt tcctttgcgc gggtttatca
    13681 gctcagactt aaagcgtggc tccgtgttat tgcccagctc ctcgcggtct tcacggatgg
    13741 caaacttacg cagtaatgca gtgatggcgc ggcggtcttt tttgcgcatg aaacacaata
    13801 ggtgccagtg aacggtgccg tcatgatgag gctcagccac ccgcacgcca taccagcgca
    13861 acccggcttt atgcatagcc ttacgaaatg cagcaaacat gccgaccaga taatcgctgc
    13921 tttgtcttac cgtcgcattt gtccaggtcg ggttgggcct gccgttattt agcgtggaat
    13981 ggaaacgtga cggacaggtg atggtgtaga aaacggcgca gtcaccgcgc attttcgcga
    14041 taagctccag acctttaaca caggccatca tctcattgcg gcgatgcgca ggattgctgc
    14101 tgctggcgtt taccacatcc tccatgtcca gcgtgtcgcc gtcttcgttc accagttcat
    14161 gagaacggaa aaactctagt gacttgcggc gctgctcacg tttatgcatc acggcttcat
    14221 agctaacata gggagatgct tttttgctga ccaggcagac agcacgcaac tgctcttccc
    14281 gccattcgca acgcatcttc cataatttcc gataccacca gtcggcgcac aacatacgcg
    14341 ccagcgaacc cggaatgagt tcatagggca cgggtttgcg gcggtttctt ttccgacgaa
    14401 gttgctcaaa cgcaggcggt atgacatcca gtcgcagggt ttccgctgct accttttccc
    14461 atgtcttgcg gatttcttct ggcttaacat catcggtggc atacaaatcg ccacaagctg
    14521 catcaaggca catgctcata tgcgcagcta ccagggtgga caggcgtttt acctgatcct
    14581 gactcatttc aggcagaatc agcaggccgt ccagcccttc atggcttgcc ataaagcgaa
    14641 aagaagtgga tagctgactg tcgcgtacat gctccagccg ttccagacat ggcttaatcg
    14701 tcttacgcaa atagcgggaa taagcctttg gcctgcccag gctgctgaag tattcaatac
    14761 gttgcatcag cggcttgctg atatgggaag gctgggcgtt aacgtccgcc agaatgacca
    14821 tgtctggatt aaaacgctgc tgctcatgcg ccagctttgc ccggctaatg agcttatcct
    14881 gctccatttc gcgttggaca ggatcacggg attcattaaa gaaataacgc tcccagacct
    14941 gatcactcag tgcctcgcgg cgcagctgtt cctgctcgtt atcggtagcg tacagagtga
    15001 tcaggtttga aagcgcagaa accggcgcaa cttccgccgg gtccagataa gggttaatag
    15061 cctttttcgg gctgttccat gagaatgctg cggcgacctc gttaacgccg ctgcaattgt
    15121 tcatatcggc atgactcatg cacgtactcc gtacacggca gaactatcca cgccacgcga
    15181 ataatcaaat cccacccagc agcgcggccc ggaaacagca atgatttctg ttgctgattt
    15241 accctcgcca gctgccacac cgatgctgcg ttttaccttg atatagtggt gagtaaaatt
    15301 gcgatacagc gaacggatca gggatgtgtc actgttagaa acaatgaccg gatgtccttc
    15361 tgatgaccga tgttcaagaa cggatgcaag gtgatactgg tcatcttcag tgaaaccgtc
    15421 agtgtgataa ccggaaaacg taccgtcata cggcggatcg caatacacca catcccccgc
    15481 cttcaacatc gccagcgttt catcaaagct ggcgcagata aacgttgccc gctgggcttt
    15541 ttctgcaaat gcgcgaattt ctttttcagg gaaatacgga tttttataat taccgtaggg
    15601 aatgttgaaa tgcccgctct tgttatagcg acataaacca cggtaaccgt gacgattgag
    15661 atacaggaaa tataccgctt tcatgaaatc agtaatttca gttgagtaat taaactcctg
    15721 ccttatgttg taataagcca cctccctgtt tgcgatctca aataaaactc tggcgcgaga
    15781 tataaacgat tcacaatcag cagcaacctt tttatagagg ttgattaaat caggattaat
    15841 atccgcaaca agatagctgg ggtactccgt ctccatcatc actgcacagg aacccgcgaa
    15901 aggttcaacc agtcgcgggc cagcaggaag gtgttttttc agttcggaca taattgcggt
    15961 tttatttccc gcccatttca ggatggtgct catacagcac ctccgttgta atgtttgcct
    16021 ttcagctctg cgatttcctg acaggtaatg caaagctgca ctccaggaat ggcgcggcgg
    16081 cgtgctggcg gaattggcac ttcacattca atgcaaagca cgcgagacac gcccggtgtt
    16141 ttggcacggg cagcacgaat atggcgctgg cgttcttctt caacgcgctg ctgtacgaga
    16201 tccattgcat cagccattag tggatctcct gcgcttcgtt ctggattgct tcagcggtca
    16261 cacgcagcag ttctgccgct tcgacgtggt ttagctggtg ggatgtgata tgacacgcaa
    16321 gactatcaag gcgggcagcc attgcttcag cccttgcccg gcgttcttcc agacgagcct
    16381 ctgtcagtaa aatatttagc cctacgtcat ccggtccggt tttagtcgtg agggtttcaa
    16441 tattacgcat aatcaattct cctgaattta gataaaggga tgcccggcgg gtttacgcca
    16501 ttaatttcat tagttggtta attcggcatg gttagccgtc tgggaaataa gctcaccact
    16561 gcacgaaaat gattcattgc tttaatcaac tcccgctttt cgtcagtggt cagctcatta
    16621 atgctgatgc tatgacgttc agctggaatt tttgccataa agaatatggc agccagtgcc
    16681 cgtttatttt gttcgctatt aatatcccgt ggatcacgca tatctttaat aaaccgttca
    16741 agctctgact caatattaag accaaaaact ttcgccctta actccgcaat atgattaagt
    16801 ccattcaggc gttcaccggg gcttaatgga acagtcgccg cagcgccttc aatagccatt
    16861 tgttcccccg ttttttcgta gatagttctg ccagcaattc atcttgtgaa cggcacggat
    16921 gccagcgttt accatcctca cccatgatcc agccgtgacc gtagtgcatt gccgggcttt
    16981 gtttaaccag cagcgatgca aatgatggtt ctttcgtaag cataagcacc tcacagcaaa
    17041 ccgaatgaag caccgaggcc agtcacggta tcaactgcac tcgccatcgc agggttagcc
    17101 tgtaaacggg cctgcagtga aacagcagcc agcgccatca gtcgtgttac agagttaatg
    17161 ctgctgatag catcacgacg acctgcactg gtttttacat cgccagatac cgcacctgca
    17221 gcaacacgcc cgatctctgc ggttgcactc atgacgtaat gcggcagttt ctcttttgct
    17281 acctcattaa tcggtacaca tggcaggcag tgaatctgtg ccagaaaacc atctatcagc
    17341 gttgaatctt cagtcagatc ggtaagcagc cagatttctg gcgcattgag ctgatgcggt
    17401 tgatctgggt tgagtttgtt tcgcagagtc tggacattca ttcctgcacg ttctgccaac
    17461 ttcgccatat tgtgacgaag tgcaaaagct ctacaggctt catcaaaatg cgggtgtttg
    17521 gaaatcttat aatcaaacat gctaccccct tagaaagttc tcataattga actcacttac
    17581 caacaatgac gcggaagttg gaatgaccga gggattcacg gacctgatcg gttttgtaca
    17641 ttaaataacg cagacttacg cggcctttgt ttttttcttt cttgaccatg tatttagcaa
    17701 gctgaccatg gtgaattttt tgatacacgg agccgcggga gataccttcc cattccgcga
    17761 actctgcagg cgtagccatc tcttttggta cacgaattga aatatcagtg ctcatagtgc
    17821 agtatctccc gattaaggtt tggtttacgt cgttttatct cgttttactt gattcaatat
    17881 ttgatacatc gagatactac gatccaatat ttgatacgtc aacaggatta aaaaatgata
    17941 caggtaaagg ctggagagaa tacaggggga agagaggcta tccatagact aatggcagcc
    18001 tatgatttca agtccagaca gcaactttgc gatcacttag gcgcatcaaa aagcaccatg
    18061 gcaaacagat acttaagaga tagttttcct gcagagtggg tgattcagtg cgccttggaa
    18121 acaggagttt ctttactgtg gctaaccacc ggacagggag agccaggtcc aaacattgaa
    18181 cctaaaaaaa atatcaattc cgtgaactcc agcaaggttg tacctctttc tgaactagta
    18241 tctcctgaaa ttgacaaggc gactctcaac ggtggtttat tggtcgatgc tggaaaagca
    18301 atcattgata gcagcatact cccctcagac tcaagcaacc tactgctggt gactacttct
    18361 ggtgattctt atttaataga tcgcaaccaa acaccaccag taaatggtat gtggttagtc
    18421 gatatcgacg ggataaaaag cattgttaaa ttgactcggc tcccgggaaa caaattagta
    18481 gtgcatcagg atgattcatc gtttgagtgc ggtctggatg acattgaggt agtaggccgc
    18541 gcactgaaaa tcattaagag cctttgatat gaccatcaga aaacagccga acggaaaatg
    18601 gttgtgtgag tgctatccca atggacgcaa tggcaagcgc gtgcgtaagc aatttgctac
    18661 gaaaggcgaa gccattgcgt ttgaaagctt cacaatggaa gaagtgaata aaaaaccgtg
    18721 gttgggtgaa aaggatgatc ggcgacgcct atcagaatta attgagcttt ggtattccct
    18781 gtatggtcaa acactcgcag accccaagcg cctcatggcg aaacttagaa ttatctgtaa
    18841 tggtctaggc gatcccatcg cctcagaact gacagctggt gactttacaa aataccgcga
    18901 agcacggtta aaaggtgaaa tacgaaatga agatggcacg cttatgtcgc ccgtaaagcc
    18961 ccgcacggta aaccttgaac agcgcaatct atcatctgtg ttcggtacat taaaaaaact
    19021 aggacactgg tcagcaccaa acccgctggc aggacttccg accttcaaaa ttgccgaagg
    19081 tgagctggct tttctttcca cggacgaaat caagcgcctg ttggcggcat gtgctgaatc
    19141 tcaaaaccct agcttactaa tgattgcaaa aatatgccta gctactggcg cacggtggag
    19201 tgaagccgaa aatctgcagg gccaccaaat atcgaaatac cgaattactt atacaaaaac
    19261 aaaaggcaag aaaaacagaa cagtacccat atcacaggag ctgtatgacg aactcccaaa
    19321 gaacagaggg aagttattct caccatgtag aaaagccttt gaacgagcag taaaacgggc
    19381 aggtatagat ctaccggatg gccaatgcac ccacgtgctg cgacatacgt ttgctagcca
    19441 ctttatgatg aatggcggga acatacttgt gctaagggat attttgggac attcggatat
    19501 caagatgacc atggtttact cacatttctc accagatcat cttgaagatg ccgttactaa
    19561 gaacccatta aacttgatat aaaacagcca aaattctatt tcatcaagta atccaattca
    19621 tccttcatag gtataacttg ctcataaagt ctatggtagg attttccaat tgcaccccta
    19681 aattgaataa gtttagggga aaagctaccg atattaataa tatgttcttg cgctatacca
    19741 ggatatggat cgataaaaat aatctctgtc atacctaact gataagcttt tttagcacaa
    19801 agttcacaag ggctagctgt agtatatagt ttccctccat taataccgac cccaccatat
    19861 ttggccaatt gcaaaaaagc attctcctct gcgtgcaatg cacgggtatg cacttgattc
    19921 cctttctttt catcatcaag gctgttgtga atatctttaa aacaatatgg caaattaagt
    19981 cctttaaaaa cagtagaact tgaatcactc gcccttattt ttattaaatt ttctttaact
    20041 ttactcctaa attttgtatt acttcgttcg tacaggctat atgttccctc atcaaaatca
    20101 tgcaataacc catcaaatga cctcatagaa caaggaactt gaccttttgc tacatcgttc
    20161 caaccaactg acttgataga gttatcatta tctgtcacaa ccgcccctac ttgcctagat
    20221 atacatccag aatttagttt tacagtatat gcaacttgca taaccctttc catggcggta
    20281 ggagatatta atccagggtg ttgcattaat gatacatacc acgcaatttg tgctttgaga
    20341 aaattattat tatcaaattc ttttttaggg ttaaaaaaat gtatatctga aatttcaatg
    20401 cattttttta cattaggatt agtaagatga ttatactcgc catctccttt accagattct
    20461 tcatcattaa ttttttttat ctgttcctct gagaacttat gtaatttctt taaatatctg
    20521 gttcgatgtt catctggtgc atttatagaa accagatgga atgcagcata tctatctttg
    20581 aagaatttag cttcatacgg gttacgtatc gcatcaatga ctatatacgc tttattattc
    20641 tttacttttc taattatttt aataacacga ttaattgttt caggtaaatg aaaaacagcc
    20701 tttggaataa agtcttgatt ttcataatct acatcaacct tccctattct tctaattgac
    20761 tttcctgctg actgatatag ctcaatatat aaacctggct ctagttcatt taattccctt
    20821 ttaaaatccc gtgtaaattt tcgagtaaga gttaatatcc taataaaggt atctttcccc
    20881 ctgcctttaa agtcaatctc atcatcatga ttaagaagat tttttatcac ctcaagatat
    20941 tttatcttca acaaactttt agcgaacaca ccattaacaa ccagtttctt ggctgcctta
    21001 aaggaaatcg aattttcttt tttatcgggg attttacttg cagaaaactg aatgaattta
    21061 gctaaggccg atggagataa aaggagcaaa tatattgaaa tgagatcact gacctttatc
    21121 gaatagaagt tcttccaatg agtctctgca aaatttttta ctatttcata acgccgcaaa
    21181 tccaatcctt tataatactc agccaagacg ttactatctg gaaaagatat ttctttttgt
    21241 tctaaaatat ttgctgcagt agtacatcct gagccagttc tcccagttaa accaataaga
    21301 ataaattgac tattttctaa aaaaagctca ctaataaatt tgtgttctct catttcgcac
    21361 ctgttacgtt ataacacaag tctcttccat gttgtattta ttagtattta ttaaaaaaca
    21421 agaaattgca acatagcggc aacaatacac acaccaagaa aatggcggca acttggcggc
    21481 agagctttaa aaacgtataa aacggataaa cacaaaacaa taccaaccaa ctgtttttga
    21541 aaacaaatta ttgttttcat tataatataa atggtatgta ggaatttcgg acgcgggttc
    21601 aactcccgcc agctccacca atcatgattg gacggtgtaa ggacaacacc aacaaaaaca
    21661 agaagttaga agtctcagca aaacaccgac cagatggtga ggagacataa aaggatacgc
    21721 aaaggagccg cggctcctgg taacatgaaa gcccacagat gtgggctttt tcgttgatgg
    21781 tcagaacgac cagttcacac cagctaccgc gttccacggg gattccacac cggcactatg
    21841 gctatacccc accccaagat gcccgcttaa cgtactgctg aatgaggctt taatacctgc
    21901 ctggtatatt ccacgtctgc ccgacaaata attgacgaaa ttaccgtcac tattcacttt
    21961 cacccggtta tcatcgacaa attctttgcg cacagccgcc ttcagccacg gctcaacttc
    22021 cataccgttc cccagacgca tgttgtaact cagcgttgcg cccagttcac gatatatact
    22081 gcgggtatcg actgatttcg atttcatgcc attggataaa tgatattcgg ggttatcagc
    22141 ggtgaacccc gttaacgatg catacggcgt caggttccag ttaccatcgg taaatcgcat
    22201 cccggtttca atgtgaccgc ccagcccgtt gctgtggtaa ctgccattgg cggctccacc
    22261 gctgctcatt ttacctgcta cgttactttt aaaacggttc agcttcacga caccgtccag
    22321 atagaaacca ctttcatgtt cccaactggc atagccgccc agagaataac tgcccacact
    22381 gccatgtcct ccgcgatcaa aaccgatatg tgaatgggaa tagcccataa aagcgcctag
    22441 cgtggtaatt ccttcaggaa tatcattacg gctgtcgatc cccactgtca ttccggtcag
    22501 cgtctgctca aacccggcac ccgcatcggt ggtgacatta ttacgggtgt tatacgtcgc
    22561 cccccagaca ttattgttgt gtggactcgc tttcattatg ttcaaccgct cgcgaatact
    22621 gtttagctca gcatcaaata ccaacggtaa tgttgctgcc atattgagta cggctgccgt
    22681 agaaggcgta atgcgtttct ccggaacggg tgtcggcgtt ggatcgggag tagggtctgg
    22741 ttttggattt gggtctggct ttggatcggg ttttgggtct ggctttggat ttgggatggg
    22801 gtccgggttg ggtttgacat cattggtcag gttccagttg ctgttgccgt cacttttcag
    22861 gacatactca taggtcccaa gatcaacgaa accgccggta ttgcccagcg taaacgaagc
    22921 atcccctccc cctgttttca ccagcgtcat cgcgtcgtca gactgtgggc tgacgccggt
    22981 atcctgaaca aagattttaa aattaccagt ggcgttgttg ttgacgacca gttgatcgcc
    23041 ccgggagcct gcaacgttgg tatgcaggta gaaattaccg ctaccggaaa gttcattggt
    23101 tgtcagcgta ttgtagatac cgttagttgt ggcaggcgct gctgtttgtg ctgacaaata
    23161 aacatcaccg tcgttgagta gcaaactgtt tactctatat tcgcagttgc tggtacctgc
    23221 acaggaatta ttgctgttaa gccagacact gccccggctg gcagccgtca ggtcggcaag
    23281 cgtggtatcg acgccatttc ccagagtcaa tgtcgcgcta tcggtaatcc ggaccgcccc
    23341 ttcgagttta actggcgtaa cattatcccg tggcgtcatt aacgacaggc ttcctgtcgc
    23401 gccactgacc gatgcatccg ccagcgtacc tgcgtagacg attgccgtac cgccagcgac
    23461 ctggagatct tctgcccggg ccagagcctg aaactcatct tttgaccgcc caagggtata
    23521 ttgcccgcca gagttaacct ttgtggcgga gtcctgaccc aggttttgca ctgccccacc
    23581 cttgccaacc gtggagtcgc gagcttcggt acctgctaat accaataaat taccgccatt
    23641 ttccagcaac atattggtcg ctaaattgcc ggaaatggaa aaagtaccgt actggtgagt
    23701 accgctgatt tcaataccgt tagccgtgct cgtctggaga gcggcaccgc tgttctggac
    23761 gatatctgtc gctttgccat tatcgttgac tgtcagcgta ccgccttcat tgatctttgt
    23821 ttttattgcc tctccgttag ctgaaactgt ttgtattccg ccgtcgttaa ttgttgtctc
    23881 attcgccaca ccctcgacaa tttgttcacc gccggtgagc gtcgtgcctg tcgcagtggc
    23941 ttttgttttg acgatctccc gtccgcccat attgacctgt gttttgtcag aagaggtgtc
    24001 tgactccacg gttaacacgc cgccatttgc cagcaggata ttgttcgccg caccctgctc
    24061 gatgctgaac gcgacgccat ccgcgcgtgt tcctgtgacc cgcgtcgccc tggtggttgc
    24121 aaccaaagcg ccctggctac tctgctgtat ccccgttgcg ctgcctttct cccgcacatc
    24181 gagtgtgccg ccgtcattaa gcaccgagtt ttcagccaga ccgccctcat taactacctg
    24241 tgaaccccca ttaataatgg aaccttccgc tgtcccgttt gccataattt gttgtaggcc
    24301 agagacgata tcggtattga ttgccttacc ataattctga acggtttgcg tgccaccatt
    24361 gatgtgtgtt ttctctgttg acccaccatc aacaatttgt tcaccacttt tgatatttgc
    24421 ttcagtggct aaaccatata ccgtttgctt gccacctttg atatttgctt tatcagaagt
    24481 ggcactggca tatattgttt gggtgccagc actattgagt acagtgccaa catcttttcc
    24541 ataaacatcc attttgccgt tggcattaat aatcgtgtca actgcgcggg aaccagtgac
    24601 gactgttaat gagccagcgt tttccagcac tacattttta gcttctgaat tcctgatgta
    24661 gaaagcatca ccataactgt tggttccttc gataagtgtt ccggaggtcg tggaagcaat
    24721 taatgcgccg ccggattgtt gctcaacatg cttagcctca ccaccgtcct gaacctccag
    24781 aacgccgcca ttattaagtg gtgctatctg ttttagcctc cttctggaca atcagcttac
    24841 cgccagtatc aatggtagta tttttcgccg aggttttagc cactaccgtc agttcgccgg
    24901 tattttccag cacaacataa ttagcctccc ctccggtaat agtgaagtga gagagcttgt
    24961 tgtatccttc gatatcagtc cctgcacccg tgttggcaac taaagcactg cccgtctcct
    25021 ggttaacccc atgtgcaata ccgccggtat agacaatcag cgagcctccg gcgctaatat
    25081 tgctgccaat tgccgtacca tctttctcaa caacctgccg gctcccggag gatataattg
    25141 ttgtgtcagc tttcccgccg cttttgatat tttgcgttcc gctgttgata ttggtgcctg
    25201 tggctatgcc ataattatta atattctgtg tgccaccatt aattatggta tttgtcgcgt
    25261 ttcctgcaac atccataacc ccgccattat ctattcgggt cgcatcagct ttagcattgg
    25321 ttaaaactga cattgttcct ttatctttaa taatcgtctt gtttgccgaa ccatatgcgt
    25381 ttatgtctaa atgaccaccg ttttccagca acacattgtc tgccacgtga ttgtggatgg
    25441 agaatgcacc ttcactattc gtaccgctca ccgtcgtacc gttagtgtta gtttttaaaa
    25501 ttgcaccatc gtgctgggta acattttttg ccgtaccacc actaacatca agcacgccac
    25561 cagaataaac ttcaataaca tccgaggtgc tggtgttatc aacaatttgc gtgccaccag
    25621 aatagatatg cgtatttttt gccgttgact tactattcag agactgagtt ccgccttcaa
    25681 tcgtcgtgtc cagcgcacgg ctctcatata ctctttgctc accgccattt ttaatggttg
    25741 ttgtttctac tgtgctctgt tcaacatact gtcgaccacc atttatggtt gtgttcgttg
    25801 ccagacttcc ttgtactacg tcctgagagc cagacttatt tatcgttgta ccatcagcat
    25861 gcccttgaac ttttactatc tggctaccac catcaatgag tattccattc gcactccctc
    25921 cctctacgcg tgaagcaccg cccttaattg tcgttccatt gctgataccc cctttataaa
    25981 cgtcctgatt gccactctcg attgtcgtac ctgtggaaat acccccgtca tgaattgact
    26041 gtctgccacc gttaatggtt gtattattag cctgccccac aaaattgtta tgacttccta
    26101 tatcttgata tccaccagat tcaataagac ttccattaga taccccgcca tgaacattct
    26161 gctggccatg gttgataata tgagtgttat ttgttgtacc tcgttcatcc actttttggt
    26221 tgccatctac agtctcatcg tttaccacac caataacatt agtagtgaag gcagccatcc
    26281 cgggcggggc atatatcaag gcagatatca ataaggaaag tactgagcgg cgacaataat
    26341 agggactggt cctgttcata aatttcatcc tctgaaaagt gaatactgag tagcgtttaa
    26401 gcgaccttag ctttgctgca acatcagccc acaggcacca gaccagggga ttcatcctga
    26461 agagacagcg caagtgtatt gtgttcaccg ctcatcaaag acatcatgat gaaatgatga
    26521 tattccgcat aagaatgagg cattttttaa acgcagtgcg ctgaagtgtg gttggataaa
    26581 aaagtcaatc cattcaggaa atacgggcgt attcttttct ttcgacaatg aggccgttgg
    26641 caaaataaaa tgatttacat aatcgtttct gatgaatatc ttctgctcac ataaaaatca
    26701 cacaataact ttgagatcgc agattgtttt acttttacag cattcgtccc cccattgttg
    26761 ggcaaatata gattgggcca gagcacgaaa gttaatacca cgttcgcaca gctcctccaa
    26821 cagcacgaca agatgccaca tactgcgccc cagtcggttc agtttacaga ctagcagtgc
    26881 gttccctgcc gatgatgtcc tgaccagttt tttcagtccg gacctcctgc cacggtttta
    26941 cgttgaagtg gtaaccctga gcacgcagtt cttcagtcag gcgtggtgca ccgtaacgct
    27001 gttattgctg ggtaagatca aaaaactttc aggcagctaa ggaaagttga accagacatt
    27061 agatgaaata tttcaaccaa ttacagcacc aattcagtca ctgccagccc accaaataaa
    27121 tcaaggggtt acatgaaaac gtagcccctt tttctttggt agtgacacta aaatggatgt
    27181 agtgtgaaga ataatcccgt ttactcaatc aataatacat attgtttcaa tctacgttat
    27241 tatctctttg taaaaattgc catttattaa tcattgaaaa ctgcttttag aacttgatac
    27301 aacgggacta gtcacaacag gactattctc aacgggatca tcctcagagg aactatcatc
    27361 aaagtcatca tccataaata aaatatcatc gaatggtgcc acgcccgtga tgagttttat
    27421 tttattatta cgatcagtca atactccact taaaccgttt tcgctcacag gttttaatga
    27481 tttttcatta ctcttgttgt aagcaggcgc attaaaaata cacggagtat caacatcaaa
    27541 caatgacgtt ccccagttca catattgaat atcatagtta ctgaagttct gtccagaaaa
    27601 gaagcacccc ttaaaatcca atccacgtaa attatataaa ccaccctctt ctttttggag
    27661 agtaatgtta attttggcta tctcccggac atcatcgcca tttttgtatt tgaataccgt
    27721 ttcaagatgt tctccagaca gcttgacttc aggaaataat ttgaaatcaa agcctatatt
    27781 attatgtgtc aacgtagatg aacaaaaaat ggaaaaagct tgcagtgctg aattatagct
    27841 atcgatttta tcttgaggct gagctcttgg taaaaactta tagcaacact cataaaaatt
    27901 aaacagaagc tctgaagacc ttgtattttt atcaaataat acccccttga actcattcac
    27961 aaacgcatct tttttttctt gaatatctat ggggtgttcc tttgactctg acaaagatga
    28021 aatctcatct attttgtttt catatgaatt acgtgattcc atacagacat ttggcggcgt
    28081 ttctaaaata acactacgcg tactacttgg cttaagtaaa ccaacatgaa aatcactttt
    28141 tcttatatta tcgaaaaggt tccattcatt tcttttagcg cattcaaaaa actgatcggc
    28201 attattttta ttcgataatt ttttagtttc agaaaacaca ttttcattat tttccagctt
    28261 tagtttaatg agaagatttt cccagacctg cttgcttaaa catattacgt caggctcacc
    28321 agaactaact aactcgtgat tattattaaa gtgtacgttg aataccttta agttattttc
    28381 accaacttca tatttaatac gttttaattg ttctccagct cccataatga caaaggcgtt
    28441 gccttcttga tatatacatt cagacatcat tttttgtaaa gtttcaagag caccgcgata
    28501 cgtctctgat gcagcttcct tacaaattaa ttttaaaata ctatgagcta gtagctccat
    28561 gtgtttagaa gatttatttt cattataact tccactaccc aaaactgcgc tagcgttaaa
    28621 tccgttgctt ttactaacta acattgtcta ttcctcaatt aatgtctaca tggctatttt
    28681 taatgttatt actgtttgtc actataaaaa aacgctcatt tgagacaatt actgacatta
    28741 actgcttcac ttgctacgca tggaactttt aattaaatta gcacaggaat gttaaattta
    28801 ataaacaaaa ggttatttcg ctgtatgata aaaaaaaccg ttataattta ttagtgaaaa
    28861 tcgtttttca agtgttagaa atttatatct caatagcgtt ggttaatgag catagccaag
    28921 ctcctgtaac gctcacaaaa ctcatctgcc tgcggcgggt gttctggtca gtagtagatg
    28981 tttaaggcgt ggcagagaca tttcatcctt actctacggc attgttctac atacattggt
    29041 tgtggtactc acttatcatc agtgagcgaa cagagaatag ttcagtgatt tgagtaatta
    29101 acctgattaa atgaaggggt ataataaatg ataatactct ggctttatcg ttaattactt
    29161 aattccacat gtaagcaatt tgcccgcttg gcatagcagg cattttttcc aggtactttt
    29221 gaatgagtac tgatggataa atacattgca gtggcgtgcc acgtaccaaa acaccagccc
    29281 tcattcgaaa ccacccaccg cacttcttcc ttgaaacggc gttagtcatg aaatatagac
    29341 cgccatcgag taccccttgt acccttaact cttcctgata cgtaaataat gatttggtgg
    29401 cccttgctgg acttgaacca gcgaccaagc gattatgagt cgcctgctct aaccactgag
    29461 ctaaagggcc ttgagtgtgc aataacaata cttataaacc acgcaataaa catgatgatc
    29521 atatgatgta aataacagat ttttatgcgt tcccattcac cttgggtcgt gattacacgc
    29581 gacataaaac ccgacaccgc ctccattcgc aaagtcgata ctcgcagtca acaagcaaat
    29641 gttaataatt agcactatct atagttatca tcgattcaat gatactttgt aatgatt
//
LOCUS       DACAPB010000038        28928 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.038, whole genome shotgun sequence.
ACCESSION   DACAPB010000038 DACAPB010000000
VERSION     DACAPB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.038"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            313..1728
                     /gene="gltX"
                     /locus_tag="IT904_001529"
     CDS             313..1728
                     /gene="gltX"
                     /locus_tag="IT904_001529"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP3191327.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1780..2151)
                     /locus_tag="IT904_001530"
     CDS             complement(1780..2151)
                     /locus_tag="IT904_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP3191328.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2174..2518)
                     /locus_tag="IT904_001531"
     CDS             complement(2174..2518)
                     /locus_tag="IT904_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP3191329.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            2754..2829
                     /locus_tag="IT904_001532"
     tRNA            2754..2829
                     /locus_tag="IT904_001532"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2787..2789,aa:Ala,seq:ggc)
     gene            3038..5227
                     /locus_tag="IT904_001533"
     CDS             3038..5227
                     /locus_tag="IT904_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAP3191330.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5277..6479)
                     /gene="nupC"
                     /locus_tag="IT904_001534"
     CDS             complement(5277..6479)
                     /gene="nupC"
                     /locus_tag="IT904_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAP3191331.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            6815..8053
                     /gene="mntH"
                     /locus_tag="IT904_001535"
     CDS             6815..8053
                     /gene="mntH"
                     /locus_tag="IT904_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAP3191332.1"
                     /translation="MTNYRVESSSGRAARKTRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8194..8520)
                     /locus_tag="IT904_001536"
     CDS             complement(8194..8520)
                     /locus_tag="IT904_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP3191333.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8635..9891)
                     /locus_tag="IT904_001537"
     CDS             complement(8635..9891)
                     /locus_tag="IT904_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAP3191334.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10095..11060
                     /gene="glk"
                     /locus_tag="IT904_001538"
     CDS             10095..11060
                     /gene="glk"
                     /locus_tag="IT904_001538"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAP3191335.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11279..11605
                     /locus_tag="IT904_001539"
     CDS             11279..11605
                     /locus_tag="IT904_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAP3191336.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            11627..12874
                     /locus_tag="IT904_001540"
     CDS             11627..12874
                     /locus_tag="IT904_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP3191337.1"
                     /translation="MAIKKRSATVVPGASDAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFSIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            12889..13974
                     /gene="ypdF"
                     /locus_tag="IT904_001541"
     CDS             12889..13974
                     /gene="ypdF"
                     /locus_tag="IT904_001541"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP3191338.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            13974..15011
                     /gene="ypdE"
                     /locus_tag="IT904_001542"
     CDS             13974..15011
                     /gene="ypdE"
                     /locus_tag="IT904_001542"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP3191339.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15036..17531
                     /gene="ptsP"
                     /locus_tag="IT904_001543"
     CDS             15036..17531
                     /gene="ptsP"
                     /locus_tag="IT904_001543"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP3191340.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNKLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIGWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(17534..18391)
                     /locus_tag="IT904_001544"
     CDS             complement(17534..18391)
                     /locus_tag="IT904_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3191341.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRSVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18404..19138)
                     /locus_tag="IT904_001545"
     CDS             complement(18404..19138)
                     /locus_tag="IT904_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP3191342.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19153..20850)
                     /locus_tag="IT904_001546"
     CDS             complement(19153..20850)
                     /locus_tag="IT904_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP3191343.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21226..22464
                     /gene="alaC"
                     /locus_tag="IT904_001547"
     CDS             21226..22464
                     /gene="alaC"
                     /locus_tag="IT904_001547"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAP3191344.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(22529..22600)
                     /gene="ypdK"
                     /locus_tag="IT904_001548"
     CDS             complement(22529..22600)
                     /gene="ypdK"
                     /locus_tag="IT904_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAP3191345.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(22956..23876)
                     /gene="lpxP"
                     /locus_tag="IT904_001549"
     CDS             complement(22956..23876)
                     /gene="lpxP"
                     /locus_tag="IT904_001549"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAP3191346.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            24229..24471
                     /locus_tag="IT904_001550"
     CDS             24229..24471
                     /locus_tag="IT904_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAP3191347.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(24548..24823)
                     /gene="ypdI"
                     /locus_tag="IT904_001551"
     CDS             complement(24548..24823)
                     /gene="ypdI"
                     /locus_tag="IT904_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HAP3191348.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            25119..25751
                     /locus_tag="IT904_001552"
     CDS             25119..25751
                     /locus_tag="IT904_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAP3191349.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            26264..27514
                     /gene="frc"
                     /locus_tag="IT904_001553"
     CDS             26264..27514
                     /gene="frc"
                     /locus_tag="IT904_001553"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAP3191350.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            27568..>28928
                     /gene="oxc"
                     /locus_tag="IT904_001554"
     CDS             27568..>28928
                     /gene="oxc"
                     /locus_tag="IT904_001554"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAP3191351.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSG"
BASE COUNT         7140 a         7008 c         7511 g         7269 t
ORIGIN      
        1 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccccgctg
       61 tgtggagtcg cattataggg agagttcaaa atgagtcaac gcattttcta aagaaattgt
      121 tcgttcgtcg taaatttaag caagatgatc gcaaaacaga ccgtgttgcg caatttgtca
      181 acgaaaacaa taatgcgtaa ggtagaaacc cgaactacat tgaggaatca ggcgggagtg
      241 atagaatatc gcccactcaa tttttccagg atttgccggt tgtcggcatc tttctaaacg
      301 taaggccatt tcatgaaaat caaaactcgc ttcgcgccaa gcccaacagg ctatctgcac
      361 gttggcggcg cgcgtactgc tctttactcc tggctttttg cacgtaacca cggcggtgag
      421 ttcgtgctgc gtattgaaga caccgatctt gagcgttcca cgccggaagc tatcgaagcc
      481 attatggatg gcatgaactg gctgagcctg gagtgggatg aaggtccgta ctaccagacc
      541 aaacgttttg atcgctacaa cgcggtgatc gatcagatgc tggaagaggg cactgcttat
      601 aaatgctatt gctctaaaga gcgcctggaa gcgctgcgcg aagagcaaat ggcgaaaggt
      661 gagaagccgc gttatgacgg tcgctgccgc cacagccatg agcatcatgc tgatgatgaa
      721 ccgtgtgttg tacgttttgc taacccgcag gaaggttctg ttgtttttga cgatcagatc
      781 cgtggtccga tcgagttcag caaccaggaa ctggacgatc ttattatccg ccgtaccgat
      841 ggttccccaa cctataactt ctgtgtggtt gtcgatgact gggatatgga aatcacccac
      901 gttatccgtg gcgaagacca tatcaacaac acgccacgcc agatcaacat tcttaaggcc
      961 ctgaaagcgc cggtgccggt ttacgcgcac gtttctatga tcaatggcga tgacggtaaa
     1021 aaactgtcca aacgtcacgg ggcagtcagc gtaatgcagt atcgtgatga cggttatttg
     1081 ccagaagcac tgctgaacta tctggtgcgt ctgggctggt cccacggcga tcaggaaatc
     1141 ttcactcgtg aagagatgat caaatacttc actttgaatg ccgtcagcaa atctgccagt
     1201 gcgttcaaca ccgacaagct gctgtggctg aaccatcact acattaacgc gctgccgccg
     1261 gagtatgttg ctactcactt acagtggcac attgagcagg aaaatatcga tacccgtaac
     1321 ggcccgcagc tggctgatct ggtgaaactg ctgggcgaac gctgcaagac gctgaaagag
     1381 atggcacaga gctgccgtta tttctacgaa gattttgctg agttcgatgc cgacgccgcg
     1441 aaaaaacatc tgcgtccggt agcgcgtcag ccgctggaag tggttcgtga caaactggcc
     1501 gcgattactg actggaccgc tgaaaacgtt catcacgcta ttcaggcgac ggcggatgag
     1561 ctggaagtgg gtatgggtaa agttggtatg ccgctgcgtg tcgccgtaac cggtgcgggg
     1621 cagtctccag cactggatgt taccgttcac gcaattggta agacccgcag tatcgagcgt
     1681 atcaacaaag cgctggattt tattgctgaa cgcgaaaatc agcagtaatt agcgtgtaaa
     1741 aagataaacg gcaggagata atatcctgcc gttttttatt tatgctgatt cgtcaatatc
     1801 gagacgggcg aggaaattgc gaattccttc acgtgacaaa aattgcgcga ctttttcctg
     1861 ctcaatgtct gtcatattat ctatcgcgtt gatgatttgt cgatacgccg gggcgcgtgc
     1921 tccaggagcg tattcagcaa ttctctcttc agcttccatc aacattggtg tgttatggaa
     1981 ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg accagaataa gtctggcacg
     2041 accgcctttc acgccgggaa atttttccgt tttccagcct ttttctctga tccaacggtt
     2101 aacagtttgt ttagccacac cgaggcattc agccagttct tcggtggtca ttttattgcg
     2161 taatcttttc atattatttg ctatcgcgaa tccctaagcg ttgtaacaat ccgataatcc
     2221 cttcccgcag aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc tctttcgcca
     2281 gtgtcaccag caatacttca agcggcgcat caccagaaag ggcaggcgct tctccctgac
     2341 cttctggacg ttcagcattg cgaatatatt cacgaacctg ttcattgacg tgaaccagtc
     2401 gggctttgcc accctggacg ccaggttttg gtgacgttgt ccagccttcc ttgcgtaccc
     2461 atttattaat ggtctggcgg ctatagccgg tcagtctggc aagttcatct ggtgtcatcc
     2521 tctccttgat cataacaatt ccctgaatgt atgtgggtct attagtagtt cattttataa
     2581 caccacttta caggaaaccg tgacatgaat aactactgaa tgcgagcggt tttccaaaat
     2641 tgttcatttg cctgattttt cagcgattga aactacacgt tgcaatttgc cgttgacaca
     2701 ttcgggcgga attcatatga tgccgcccgt caactcgaca agctttacgt gacggggcta
     2761 tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg atcccgctta
     2821 gctccaccaa atttccaacc ctcgctgcaa agcgggggtt ttttgtctct gctttttgcc
     2881 gcttttgtaa tacagtctac gtccgggtta gtgccgcctg gtgaaagcat cattggatga
     2941 aaaatcggca acaggctggc cccctgtttg cttcgcgatg cgaataaact tattatttgt
     3001 gtgcctgaaa accccgatca gtgagagtag tgtactcatg tttgtggagc ataacctgat
     3061 aaaaaatatc aagatattca cactagcgtt tacgctcacc gtggtactta ttcagctatc
     3121 ccgttttatt tcgccacttg ccattatcca ttccagttat atctttctgg cgtggatgcc
     3181 actgtgcgta atgctgtcaa tcttgtttat ctttggctgg cgcggtgtcg ttcccgtttt
     3241 atgcgggatg ttttgcacca atctgtggaa ctttcatctc tcttttttac agactgcggt
     3301 catgctcggt agccagacgt ttgtcgtgtt gtgtgcctgc gcaatattac gctggcagct
     3361 ggggacgcgt tggcgttatg gattgaccag ccgatatgtc tggcaacgtc tgttctggct
     3421 tggtttggtg acgccgatcg gcatcaaatg cagcatgtat cttgtgggga gcttctttga
     3481 tttcccgcta aagatatcca cctttttcgg cgatgcggat gccattttca cggtcgttga
     3541 tttgctaagc cttttcaccg ctgtgctgat ttacaacatg cttttctact atctcactcg
     3601 catgattgta agtccccact ttgcgcagat attgtggcgc agggatatcg ctccgtcgtt
     3661 gggcaaagag aaacgcgcat ttaccttaag ctggctggca gcgttaagcg tgctgctact
     3721 tctgttgtgc acaccttatg aaaacgactt tattgccggt tacctggtac ccgttttctt
     3781 catcatcttt accctcgggg tcggtaagct tcgctatccg tttttaaatc tcacctgggc
     3841 tgtttcaacg ctttgccttc tgaattacaa ccagaacttt ttgcaagggg tggaaaccga
     3901 atattcgctg gcatttattc ttgcggtgct gatttccttt agcgtttgcc tgctctatat
     3961 ggtgcgcatt tatcatcgca gtgaatggct taatcgccgc tggcatttgc aggcgctgac
     4021 agatccgtta acgctcctac ccaactttcg tgcgttggaa caagcgccgg agcaagaggc
     4081 gggcaagagt ttttgctgcc tgcgcattga taatcttgag tttatgagtc gtcattacgg
     4141 cttaatgatg cgcgttcact gtatccgctc aatttgccgt acgctgctgc cgttgatgca
     4201 ggaaaacgaa aagttgtatc aattgccggg tagtgaactg ctgttagtgc tgagcgggcc
     4261 ggaaacggaa gggcgactcc agcatatggt taacatcctg aatagtcggc aaattcactg
     4321 gaacaatacc gggctggata tgggctatgg tgctgcctgg gggcgttttg atggaaatca
     4381 ggaaaccctg caacccttgt tggggcagtt aagctggctg gcggagcaat cctgcgcaca
     4441 tcatcatgtg ctggcgctgg atagcagaga ggagatggtt tccgggcaga ccactaaaca
     4501 ggtgctattg ctgaatacca ttcgcacggc gttagatcag ggtgatttgc tgctctacgc
     4561 ccagccaatt cgcaacaaag agggtgaagg ttatgatgag atcctcgcgc gactgaaata
     4621 tgacggcggc attatgaccc cggataagtt tctgcccctt attgctcagt ttaaccttag
     4681 cgcgcgtttt gatttgcaag tgctggaatc cttgttgaag tggctggcaa cacacccttg
     4741 cgacaaaaaa ggaccgcgct tttcagtcaa tttaatgccg ctcacgctgc tgcaaaagaa
     4801 tattgccggg cggattattc gtctgtttaa gcgttatcac atctccccgc aggcggtcat
     4861 tcttgagatc accgaggagc aggcgttttc taacgcagaa agcagcatgt acaacatcga
     4921 gcagctgcat aagtttggtt tccggattgc gattgatgac tttggcaccg gatatgccaa
     4981 ctacgaacgg ttaaagcgtt tgcaggctga tatcatcaaa attgatggcg tctttgtgaa
     5041 agatattgtc acgaacacgc tggatgcgat gattgtgaga tcaattaccg atctggcgaa
     5101 agcgaagtca ttgagtgtgg tcgcggagtt tgtcgagacg caacagcagc aggcgctatt
     5161 gcataagctc ggggtgcaat atctgcaagg gtatttgatt ggtcgcccgc agccattagc
     5221 tgattaacag gcgtaaaaaa accggggaat tatccccggc ttttttatgt atggtcttac
     5281 agcaccagtg ctgcgattga cgcagacagc acactcacca gggtagagcc gtaaaccagc
     5341 ttcagaccga agcgagaaac cacgttacct tgctcttcat tcaggccttt aactgcacct
     5401 gcgataatcc cgattgaaga gaagttagcg aaggaaacca ggaacacaga gatgatgcct
     5461 tcagcacgcg gagagagcgt ggaagcaatt ttctgcagat ccatcatcgc aacgaactcg
     5521 ttggaaacca gtttggtcgc catgatactg cccacttgca gtgcttcact ggaaggaaca
     5581 cccatcaccc atgcaatcgg atagaagatg tagcccagga tgccctggaa ggagatgctg
     5641 tagccaaacc agccagtaac ggtggcaaac agtgcgttca gcgcggcgat cagggcgata
     5701 aagccgatca gcatcgcggc aacgataatg gcaactttga aacctgccag aatgtattca
     5761 cccagcattt cgaagaagct ctgaccttcg tgcaggttgg acatctgaat gttttcttca
     5821 ctggcatcaa cacggtaagg gttgatcagc gacagcacga taaaggtgct gaacatgttc
     5881 agtaccagcg ccgcaacgac atatttcggc tccagcatgg tcatgtatgc accaacgatg
     5941 gacatagata cggtggacat cgccgttgct gccatggtgt acatacggtt acgggagatt
     6001 ttgccgagga tatctttata ggcaataaag ttttcagact gacccagaat cagggagctg
     6061 acggcgttaa aggattccag tttgcccata ccgttgactt tggagagcag gaaaccaatt
     6121 gcgcggataa taaccggcaa cacgcgaatg tgctggagaa taccgatcag tgcagagata
     6181 aagacgattg ggcacagcac tttcaggaag aagaatgcca ggccttgatc attcatgcta
     6241 ccaaagacga agttagtccc ttcgttggca aatccgagca gtttttcgaa catttcggag
     6301 aagcctttca cgaagcctaa accaacgtcg gagttcagga agaaccacgc cagtaacact
     6361 tcgataacaa gcagttgaat aacataacgg atacgaattt ttttgcggtc gctgcttacc
     6421 agcagtgcga gaatcgcaac aacggcaagt gccagtacaa aatgaaggac gcggtccata
     6481 tttgctccaa atatgaggca ggttaaattt ccgtgcacat tctatgtaac atggttaagg
     6541 aaaacgagat ctgacacaca ctataatgac gtcctgtgac aagcttcaaa aatagtgatc
     6601 tgtcatacat ttctgctatg ttgtgtatgg aagctgaaag ttatgtaaat gtgctaacat
     6661 taaacataac cataacattc aagtcatcaa tcttttcgta gtcataatcc tggtctatca
     6721 aagaaatcac cacaacccat ttaaatgaat ttgataatca ttctcgtttg acatagcatg
     6781 aaacatagca aaggctatgt tttagaggca caagatgacg aactatcgcg ttgagagtag
     6841 cagcggacgg gcggcgcgca agacgaggct cgcattaatg ggacctgcgt tcattgcggc
     6901 gattggttat atcgatcccg gtaactttgc gaccaatatt caggcgggtg ccagcttcgg
     6961 ctatcagctc ctgtgggttg tcgtttgggc caacctgatg gcgatgttga ttcagatcct
     7021 ctctgccaaa ctagggattg ccaccggtaa aaatctggcg gagcaaattc gcgatcacta
     7081 tccgcgtccc gtagtgtggt tctattgggt tcaggcagaa attattgcga tggcaaccga
     7141 cctggcggaa tttattggtg cggcgatcgg ttttaaactc attcttggtg tttcgttgtt
     7201 gcaaggtgcg gtgctgacgg ggatcgcgac tttcctgatt ttaatgctgc aacgtcgcgg
     7261 gcaaaaaccg ctggagaaag tgattggcgg gttactgttg tttgttgccg cggcttacat
     7321 tgtcgagttg attttctccc agcctaacct ggcgcagctg ggtaaaggaa tggtgatccc
     7381 gagtttacct acttcggaag cggtcttcct tgcagcaggc gtgttagggg cgacgattat
     7441 gccgcatgtg atttatttgc actcttcgct cactcagcat ttacatggcg gttcgcgtca
     7501 acaacgttat tccgccacca aatgggatgt ggctatcgcc atgactattg ccggttttgt
     7561 caatctggcg atgatggcta cagctgcggc ggcgttccac ttttctggtc atactggtgt
     7621 tgccgatctt gatgaggcat atctgacgct gcaaccgttg ttaagccatg ctgcggcaac
     7681 ggtctttgga ttaagcctgg ttgctgccgg actgtcctca acggtggtgg ggacactggc
     7741 ggggcaggtg gtgatgcagg ggttcattcg cttccatatc ccgctgtggg tgcgtcgtac
     7801 agtcaccatg ttgccgtcat ttattgtcat tctgatggga ttagatccga cacggattct
     7861 ggttatgagt caggtgctgt taagttttgg tatcgccctg gcgctggttc cactgctgat
     7921 tttcaccagt gacagcaagt tgatgggcga tctggtgaac agcaaacgcg taaaacagac
     7981 aggctgggtg attgtagtgc tggtagtggc gctgaatatc tggttgttgg tgggtacggc
     8041 actgggattg tagttgaatg agcgtcgcat ctggcactat tggcggatgc ggcgtaaacg
     8101 ccttacccgc cctacgcgtt aaagagccgg tttgtaggcc tgataagacg cgctagcgtc
     8161 gcatcaggca ttgtgctcca ttggcgaatg tgtttaatgg cggtgtccat gtccgcggcc
     8221 ttttccgcga tgatcgtcac gatcgcgcca gccttcacga tagccacgct catacgcttt
     8281 acgcttatcc cagccacgat gatagccatt atcatgacgc caccagcggt ttttgcgcca
     8341 ctcataattg cgatgccagt aatcacgatc tcgccagtga ccaccgtccc agtaattacc
     8401 gtaatgatcg cgatcgccaa tttgtaattt gattgatggc agtagggtga tttcacctgc
     8461 gtttgctgca agcggggtaa atgccatcag ggcggccgcc agaaacagtg acctgaacat
     8521 tgttattctc cttcacgatc gaagccgtca gcggcctgtt aacgcaatat tacggggagg
     8581 taaagccccg tatcatcgcc ataactctta aatcataaag gagagcattt tttgctaaac
     8641 gttatcgtgg gggggctgtt gcttcggacg attgaccatc atcatcggct tacctgctaa
     8701 taacagccat gccggaagca tgacgataca gagcagcggt agcaatgtgg tattgggtac
     8761 aacgactgcc gccataaaaa gacttaacca gccatcgcgt gttaccacca gcacgatgcc
     8821 gagaatagcg caagaaacgg ttattgccgc tggtacggcg ggaacgtgct catgcagcat
     8881 caaccctaat gccacgccga caaacactgc cgggaaaatt cgcccaccgc gaaagccact
     8941 ggcggcagca acgaccaggg cggcaagttt aattaccgcc agcaaaaagt aatcgctggt
     9001 gctgaaagcc tgatttgcca ccatctgctg catctcatcc agccctttaa acagcgaaac
     9061 tggtccacca ataaccccca gaataccgag aataaatccg ccaataccca gcacgagcac
     9121 cggatttttc atttgatgca tcatcgcgtg caaccgtggt aagcaccata cggcaaccat
     9181 ccctgcggcg atggcaatcg ccgcgacaat tgcgccgctg agaatatcgg tcatttccat
     9241 ctgtccgtaa tgagcaatgg gcagtgaaaa atgaggatgg aaaaataatc cggtagtaag
     9301 tgcaccagct gctgccgcca ttaacggcgc aaaaagacga tcccatagcg gaacttcact
     9361 actgccattt aaggtttgcg aaaatatcaa cgccgccgca acaggtgtac caaacagcgc
     9421 gccgatggtt ccggcagagg ctaaaatagt ccactccatt cggttgacgc gcggtaacag
     9481 acgagcgcca atggcaaccg caagggcgat attgacggtc atgatcggat gttccggccc
     9541 caggctgacg ccgccagcaa gaccgagaat taatgctacg ataagtccag gtagcgcaga
     9601 gggcggaacc ggtgcgccga tcagcggttc acaggcgggg tctggtccgg catgaccctg
     9661 gctgaaacgg ataaccaacc ccaccgcaat acccgttagc gttaatacac cgatgatcca
     9721 gaggggtgaa tcctgggcta tccccagagt tcccggcagt cgttgccaga gcaaattctg
     9781 taataccgag gcgattttca tcaccacaat cagaataaga ctggacgcaa tcccaattgc
     9841 cacggcgggg agcgataata acaacatggt tctggctcgc ggatggagca tgttctattc
     9901 cttatgcggg gtcagatact tagtttgccc agcttgcaaa aaggcatcgc tgcaattggt
     9961 gctgaaacga taaagtaatt gtgtgaccca gatcgatatt tacagggagc ctgcctttcc
    10021 ggcgttgttg ttatgccccc aggtatttac agtgtgagaa agaattattt tgactttagc
    10081 ggagcagttg aagaatgaca aagtatgcat tagtcggtga tgtgggcggc accaacgcac
    10141 gtcttgctct gtgtgatatt gccagtggtg aaatctcgca ggctaagacc tattcagggc
    10201 ttgattaccc cagcctcgaa gcggtcattc gcgtttatct tgaagaacat aaggtcgagg
    10261 tgaaagacgg ctgtattgcc atcgcttgcc caattaccgg tgactgggtg gcgatgacca
    10321 accatacctg ggcgttctca attgccgaaa tgaaaaagaa tctcggtttt agccatctgg
    10381 aaattattaa cgattttacc gctgtatcga tggcgatccc gatgctgaaa aaagagcatc
    10441 tgattcagtt tggtggcgca gaaccggtcg aaggtaagcc tattgcggtt tacggtgccg
    10501 gaacggggct tggggttgcg catctggtcc atgtcgataa gcgttgggta agcttgccag
    10561 gcgaaggcgg tcacgttgat tttgcgccga atagtgaaga agaggccatt atcctcgaaa
    10621 tattgcgtgc ggaaattggt catgtttcgg cggagcgcgt gctttctggc cctgggctgg
    10681 tgaatttgta tcgcgcaatt gtgaaagctg acaaccgcct gccagaaaat ctcaagccaa
    10741 aagatattac cgaacgcgcg ctggctgaca gctgcaccga ttgccgccgc gcattgtcgc
    10801 tgttttgcgt cattatgggc cgttttggcg gcaatctggc gctcaatctc gggacatttg
    10861 gcggcgtgtt tattgcgggc ggtatcgtgc cgcgcttcct tgagttcttc aaagcctccg
    10921 gtttccgtgc cgcatttgaa gataaagggc gctttaaaga atatgtccat gatattccgg
    10981 tgtatctcat cgtccatgac aatccgggcc ttctcggttc cggtgcacat ttacgccaga
    11041 ccttaggtca cattctgtaa atccttcctt ttatatcggg aggtaactct cccgataatc
    11101 ttttaaatca tacagtttat tcaatttttc tttgtgtccc ctcacaaggc cgacctgcgt
    11161 cacacttccg tacagcggga ttaattctcc agtaaatgca ttatttgtct ggtaacggcg
    11221 atttgttttg cacgttcata atttcactcg tcaactatca ctgaacgagg aattaacgat
    11281 gagtaagaaa ctgattgcct tatgtgcctg cccgatgggc ctggctcaca cctttatggc
    11341 cgctcaggcg ctggaagaag cggcggtaga agccggttat gaagtgaaaa ttgaaaccca
    11401 gggcgcggac ggtatccaga atcgcctgac ggcgcaggat atcgccgaag cgaccatcat
    11461 catccactcc gtggcagtta ccccggaaga taacgaacgt ttcgaatcac gcgacgttta
    11521 tgaaatcact ttgcaggacg caattaaaaa cgctgcgggc atcatcaaag aaatcgaaga
    11581 gatgattgcc tctgaacaac agtaatattc gacagggata attgttatgg ccattaaaaa
    11641 acgcagtgca accgttgtgc ctggcgcatc cgatgcggca gcggcagtta agaatccgca
    11701 ggcctctaaa accagcttct ggggtgaact cccgcagcat gtgatgtcag ggatttcacg
    11761 catggtgccg accttaatta tgggcggtgt gatcctcgct ttcagccagc tgattgctta
    11821 tagctggctt aaaattcctg ctgagatcgg catcatggat gctcttaata gcgggaaatt
    11881 ctccggtttc gacctctcct tacttaaatt tgcctggctg tcgcagtcct tcggcggcgt
    11941 gttgtttggt ttttccattc cgatgtttgc tgcttttgtg gcgaactcta tcggtggcaa
    12001 actggcgttc ccggctggtt ttatcggcgg gttgatgtct acccagccga cgcaactgct
    12061 gaacttcgat cccagcacta tgcaatgggc gacctcttcg ccggtgccgt ccaccttcat
    12121 tggtgcgctg attatttcta tcgtcgcggg ttaccttgtg aagtggatga accagaaaat
    12181 ccagttaccg gatttcctgt tagcgttcaa aaccacattt ttgctcccga ttctttccgc
    12241 catttttgtc atgctggcga tgtactacgt catcaccccc tttggtggct ggatcaacgg
    12301 cggtatccgt actgtgctga ctgccgcagg tgagaagggc gcgctgatgt acgcaatggg
    12361 gatcgccgcg gcgacagcaa tcgaccttgg tggcccgatc aacaaagccg caggttttgt
    12421 tgccttcagc tttaccactg accacgtatt accggtcacc gctcgttcta tcgctatcgt
    12481 tattccgccg attggtctgg ggctggcgac cattatcgac cgtcgtttaa ccggcaaacg
    12541 cctgttcaac gctcaacttt atccgcaggg taagaccgcc atgttcctgg cgtttatggg
    12601 gatcagtgag ggtgcgattc cgtttgcgct ggaaagcccc atcaccgcca ttccgtcgta
    12661 tatggtcggc gctattgtcg gctcaaccgc cgctgtctgg ctgggcgcag tgcaatggtt
    12721 cccggagtcc gctatctggg catggccgct ggtcactaac ctgggcgtct atatggcggg
    12781 gatcgcgctg ggggcagtca taactgcgct gatggtggtg ttcctgcgtc tgatgatgtt
    12841 ccgtaaaggc aaattgttaa tcgatagcct gtaagaagga aaatccagat gacattactc
    12901 gcttcgctgc gcgactggct taaggcgcaa caactggatg cagtgcttct ctcctcacgg
    12961 cagaacaaac agccgcatct ggggatctcc accggatcag gttatgtggt gattagccgt
    13021 gaaagtgcgc acattctggt ggattcgcgc tattacgttg aggtggaagc ccgtgcgcaa
    13081 ggctaccagc tgcatttgct tgacgcgacg aacacgctta ccactatcgt caatcaaatc
    13141 attgccgatg aacagttgca aacgctcggt tttgagggcc agcaggtgag ttgggaaacc
    13201 gcgcaccgct ggcagtctga actcaatgcg aaactggtta gcgccacgcc ggatgtgctg
    13261 cggcaaatca aaacgccaga ggaggtggag aaaatccgcc tcgcctgtgg gattgctgat
    13321 cgcggtgcag agcatattcg ccgctttatt caggcgggga tgagcgagcg cgagatagcc
    13381 gctgaactgg agtggtttat gcgccagcag ggcgcagaaa aagcctcttt tgacaccatt
    13441 gtcgccagtg gctggcgtgg ggcgctgccg cacggcaaag ccagcgacaa gattgttgca
    13501 gcgggcgagt ttgtcactct cgatttcggt gcgctgtatc agggctactg ctctgatatg
    13561 acgcgcacct tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc gctgtttaac
    13621 gtctatcaaa ttgtcctgca ggcacagctc gcagcaatct ccgcgattcg ccccggcgtg
    13681 cgctgccagc aggttgacga tgccgcgcgc cgggtcatta cagaagcagg ttatggcgac
    13741 tatttcggtc ataacaccgg tcacgctatc ggcattgaag ttcatgaaga tccgcgtttt
    13801 tcaccgcggg acaccacgac gctacagcca ggcatgttac tgaccgtgga gccggggatt
    13861 tatttgccag ggcaaggggg cgtgcgcatc gaagatgttg tgctggttac cccgcaaggc
    13921 gcagaagtgc tctacgccat gccgaaaaca gtgttgctca cgggagaggc ataatggatt
    13981 tatcgctatt aaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa caggaagtgc
    14041 ggcagatcct gctggaagaa gcggatcgcc tgcaaaaaga agtgcgattt gatggtctgg
    14101 gatcggtgct gatccgcctg aatgaatcga caggtccgaa ggtgatgatc tgtgcgcata
    14161 tggacgaagt gggatttatg gtgcgcagca tctcccgcga aggggcgatt gatgtgctgc
    14221 cggttggcaa cgtacgcatg gctgcccgcc agctgcagcc ggtgcgcatc accacccgtg
    14281 aagagtgcaa aattccaggc ctgcttgacg gcgaccggca ggggaatgac gtcagcgcca
    14341 tgcgcgtgga cattggtgcg cgctcctatg acgaagtgat gcaggcggga attcgtcccg
    14401 gcgatcgcgt cacgtttgat accacttttc aggttctccc tcaccagcga gtgatgggga
    14461 aagcctttga tgaccgcctc ggttgctatc tgctggtgac gttactgcgc gaactgcacg
    14521 acgccgaact acctgcggaa gtgtggctgg tggcaagttc cagcgaagag gtgggattac
    14581 gcggcgggca aactgccacc cgcgcggtgt cgccggacgt cgccattgtg cttgataccg
    14641 cctgctgggc gaaaaacttt gattatggcg cggctaacca tcgccagatt ggtaacgggc
    14701 cgatgctggt gttaagcgac aagtcgctga ttgcgccgcc aaaacttacc gcctgggtcg
    14761 aaaccgtggc ggcagaaatt ggcgtgccgt tgcaggcaga tatgttcagc aacggcggca
    14821 cggacggcgg ggcggtgcac ttaaccggca ccggcgtgcc cacagtggtg atggggccag
    14881 caacccgcca tggacattgc gccgcatcga ttgccgattg ccgcgacatt ttgcagatgc
    14941 agcaactttt atctgccctt attcaacgtc ttacgcgtga gacggttgtt caactgacgg
    15001 atttcagatg atctcctgat taacccggag cggttatgtt aacgattcaa tttctctgtc
    15061 ctttgcctaa cggtctacat gctcgtccgg cgtgggaact taaagaacag tgcagccagt
    15121 ggcaaagcga aatcactttt attaaccatc gccagaacgc aaaggcagat gcgaaaagct
    15181 cgctggcgct gattggcacc ggcaccctat ttaatgacag ttgcagcctg aacattagcg
    15241 gcagcgatga agagcaggcg cggcgcgtgc tggaagagta catccaggtg cgctttatcg
    15301 acagcgacag cgttcagcct acgcaggcag aactgacggc gcatccgctg ccgcgttcat
    15361 taagccgcct taacccggat ttactgtacg gcaatgtgct ggcaagcggc gtcggcgtgg
    15421 gtacgctgac cctgttacaa agcgacagcc tcgacagtta tcgggcaatc cccgccagtg
    15481 cgcaagattc cacccggctg gagcacagcc tggcaacgct tgccgagcaa ctgaaccagc
    15541 aattgcgtga gcgtgacggc gaaagcaaaa ctatcctcag cgcccatttg tcgctgattc
    15601 aggatgatga atttgcaggc aatatccgtc gcctgatgac agaacagcat caggggctgg
    15661 gggcggcgat catcagcaat atggagcagg tttgcgccaa actttctgcc tctgccagcg
    15721 attatctgcg cgaacgtgtt agcgacattc gcgatatcag cgaacagttg ctgcatatca
    15781 cctggccgga actgaagccg cgcaacaagc tggtgcttga aaaaccgacc attctggtgg
    15841 ctgaagattt aaccccaagc cagtttttga gccttgattt gaaaaatctt gcgggcatga
    15901 ttctggagaa aaccgggcgc acctcgcata cactgattct ggcccgtgcc tcggcgatcc
    15961 cggtactgag tggcttgccg ctggatgcga ttgcccgtta tgccgggcaa cctgcagtgc
    16021 ttgacgccca gtgcggcgtg ctggcgatta acccgaatga cgcggtgagc ggttattatc
    16081 aggtcgcgca gacgctggcg gataaacgcc aaaaacaaca ggcgcaggct gccgcgcagc
    16141 tggcctattc ccgtgataac aagcgtattg atattgcggc gaatatcggc accgctctgg
    16201 aagcgccagg cgcgtttgcc aacggcgcgg aaggtgtcgg gctgttccgt accgaaatgc
    16261 tctatatgga tcgcgacagc gcgccggacg agcaggagca atttgaagcc taccagcagg
    16321 tgctactggc ggcgggcgac aagccgatta tcttccgcac gatggacatc ggcggcgata
    16381 aaagcattcc ttatctgaat attccccagg aagagaaccc gttcctcggc tatcgcgcgg
    16441 tacgtattta cccggaattt gctggcctgt tccgcactca actgcgggcc attttgcgcg
    16501 ccgccagttt cggcaacgcc cagttgatga tcccgatggt tcacagcctc gatcagatct
    16561 tatgggtgaa aggcgagatc caaaaagcga tcgttgagct taagcgcgat ggcctgcgtc
    16621 atgcagagac gattacgctt gggatcatgg tggaagttcc gtcggtgtgc tacatcatcg
    16681 accacttctg cgatgaggtc gatttcttca gtatcggctc caacgatatg acccagtatc
    16741 tgtatgcggt cgatcgtaat aacccgcgcg tatcgccgct atataacccg attacgccat
    16801 cgttcctgcg catgttgcag caaatagtta ccactgcgca tcagcggggc aaatgggtag
    16861 gcatttgcgg tgaactgggc ggtgaaagcc gttatctgcc gctactgctt gggctgggcc
    16921 tggatgagct gagtatgagt agcccgcgta ttccggcggt gaaaagccag cttcgtcaac
    16981 tggatagcga ggcgtgtcgg gaactggcgc gtcaggcatg tgaatgccgc agtgcgcagg
    17041 aaattgaagc gttactcacc gcctttacgc cggaagaaga cgttcgccca ctgctggcgc
    17101 tggagaatat ctttgttgat caggatttta gcaataaaga gcaggcgatc cagttcctgt
    17161 gcggcaacct cggcgttaac gggcgcactg aacatccgtt tgagctggaa gaagatgtct
    17221 ggcagcggga agagattgtt accaccggcg ttggttttgg cgtagcgatc ccgcacacca
    17281 aatctcagtg gatccgtcat tccagtatca gcattgcccg gctggcgaaa ccgattggct
    17341 ggcagtcaga aatgggcgaa gtcgaactgg tgatcatgct gacgctgggt gctaacgaag
    17401 ggatgaatca tgtgaaagtc ttctcgcagc tggcgcgtaa gctggtgaat aaaaacttcc
    17461 gccagtcgtt gtttgccgcg caagatgcac aaagtatcct gacgctgctg gaaacagaat
    17521 taaccttctg acgttagccc tgaaaacggg cgctgtactc tcccggcgtc agaccaaact
    17581 gacgccggaa aacgcgacaa aaatagtcgc tatccggaaa accgcaacgc tgtgccactt
    17641 catgaattga cagatgatat ttctgcaaaa tcatcctcgc cttcgccatt cgcacccaac
    17701 gcacatactc gataaaacgc atcgttccat gctgagcaaa cagttttgac agatgattgg
    17761 gcgtgatatt aaaaaactgc gccacgctct cgcgggtgag cggctgggca taattatcct
    17821 gaacccagtt acaaatgctg tgatagagaa aatcgccgcg cggctgcgaa ttgcccggcg
    17881 gcatattcac cacgctacgg caaagatgca gcaaacttaa caccagcggc tgaatgatat
    17941 tttgctccag cggcgaacgg ctaagatggg tgagggcggt aagcatcgct tcaccttcac
    18001 cgcgttgcag gctgggaagc tggatctggc gagcaggatg caacaacgac gtggtgcggc
    18061 tatcgtaaaa cgataaccca agccaggtcg gagcgaacac caggcttaac agcatcaccg
    18121 gtttgttgtt gaccggtaaa ttagccgcgc gcgccggaat aaacagcatt tctccttcca
    18181 ccaaacgttg ctgctttgct tccagcagat tgccgtattc gccgcgcagc acgatatcca
    18241 gacggggaaa atcaatacat aaactgccca ccggcaacga ggcaggctgg ctggcaaacc
    18301 agacgcgccc cagtagttgc gggttaagca ccaggccgct gaacagatcg gcgaaaaatt
    18361 gttgatcggc gggcaagcct ggtgctttca tgaaagttcc cgattaaaga tgcattaact
    18421 ggcgaaattc tttcactttg ctgcggctga ccggcacttc aaaatccaga tctttcagtc
    18481 gcagaatgta ggtgttatta aaccacggtt cgatttcgcg tattttgttc agattgacac
    18541 aaaatgagcg atggcagcgg aaaaaatgcg acggcggcag tttgctgcaa aattcggtaa
    18601 tgttcatcgg cattacgtag gattcacgcc gcgtatagac aaaggtcatt ttctcgtgcg
    18661 cttcggcgta atagatatcg ttaattggcg tgacgattat tcgctcatct ttcaccagat
    18721 taatggtgtc attctcacgc gttaccgtcg cggcaggcgt actgctggtc tgctgttgtt
    18781 gccaggccgc ttccagtttt tgcagcatcc cggtaatacg tgactcctga tacggtttga
    18841 gaatgtagtc aaacgcctcc agttcaaacg cttctaccgc atgttctttc cacgcggtga
    18901 tgaacacaat aaacggttta tgggcgaact ggctgatgtt ttgcgccagc aacacgccat
    18961 ccagcgacgg aatattgata tccagaaaaa tggcgtcgac gcggttatgc tgcaaaaact
    19021 tcaacacgtc cagaccgtcg tcaaaggtgc cgacaatctc catctggctg tgctctttaa
    19081 ttagccagct cagttcctgt tgtgccagga attcgtcttc aacaatgatg actttcacaa
    19141 tatcactccg gctcaaagca ataacgtagc ctgtgaggcg actggggtgc gttggttagg
    19201 aatgtaaaac gcaatttccg tccccggctc caggcggcgg atatgcagcc cctcgccata
    19261 caataacttc acgcgatgat ggacattcag caggccaatt ttattgcccg gcatttcatt
    19321 cgcttcgacc cgctcaatca cctttggatc gatgccgtgc ccggtatctc gcaccgcaat
    19381 gcgtacccga tttccgcact ctgcaacgct gatggtgaca acgcctttac ctttgcaagg
    19441 ctgaataccg tggacaatgg cgttctccac caacggctgg atcagcaggc tgggaatgca
    19501 gcaattcacc tcttcatcaa tatcatagat aaccgtcagc ttgtcaccaa agcgggcctg
    19561 ctcaatggca atatagtctt taatttgata cagctctttt ttgatatcga tttgctcatc
    19621 gtcttttaat tcaatgttat agcgcagata acgcgacaga ttaaagatca actggcgagc
    19681 ggtatccgga ttcagacgga ttgacgatga aatagcgttc agagcgttaa acagaaaatg
    19741 gggattaatt ttgctttgca gggcgcgaag ctctgcctta tttgccattt cacgtagctg
    19801 ctcggcgcgt gaaacctcca gttgcgtgga gatgatttgc gacagaccga ccgccatttc
    19861 ctgtaatgac gaggtgatct gatgcgcgtg gcagtagtaa attttcagcg ttccggtcac
    19921 gacccctttc tcccacaatg ggatcaccag catggaatga atctctggtg tgcggtgggc
    19981 ttcatcattg tttttaatga tgatttttcc gtaattcatc gcctgacggg tagtcgggct
    20041 aatgaagtca tcgccattct gatagttatg ttcacccacg ccaacatagg ccagcacatg
    20101 atcggtatta gtaatcgcca ccgcatcggc gtgaatgtca tcgcggataa tttcgcagac
    20161 cttgcgtaat gactcgctat tgacatggcg aaacagcggt agcgttttgt tggcgatatc
    20221 cagcgccagc ttggcctgcc gcgcggcgct ggcctctttt tcgccctcaa cgctttgcac
    20281 cagaagcaca ataaagccga tacagacgct accgaggatc attggaatgc cgattttaga
    20341 gacgatatcg atccccaacg cggtagttgg tgcccagaca attaccagaa tcatggtcaa
    20401 cgtctcacac aacatgccgc cgagaatacc gacgcgccag cgctgtgctt tggggatttt
    20461 caggttgatc cagcccgata tacaacccgc cagaatgctg gtgataaagc aggggatcgc
    20521 cgtcacgccg ccgatatcaa ttaaataccg gtgaataccc gcaatcacgc cggtaatgat
    20581 gcctacccac gggccaaaca gaatcccgcc ggacatcacc gcgataatac gtacgttcac
    20641 cagcgagcct tctacgggaa cgccggacca ggtgctgaac agggcgaaca gcgagaaaat
    20701 ggcggtaacg gcgagcaatt cttttgggga gtgcgccgac ttgtgcaaca gttcgcgaaa
    20761 caggcggata cggatgagaa agaacaggca gataagcatt aacgctgccc gatcgaagac
    20821 cgccagcagc atgttgaata tttcgtgcac gggtgactct gaataaggat taaacctcta
    20881 tgataggtaa cctgaaggct gatgaccagc aggccgtttt tgaggaaaaa ccacaatttt
    20941 aaaaggggat tgatagataa atggtgtatt agcaatgcat ctcttgataa tgcacttctg
    21001 gttagtaaat ttattgaaat tattatgtta ataaaatgag tggtaatttt cttgttagcg
    21061 cagataaatt tcctgttttt aaagcagagt tcctcattta acgtcattaa taaaatgttc
    21121 taaaacgctg gacagacaga aattaatcag gctattttca aaacagccac catcgtggca
    21181 gcgtcgctcg gacggtccgg gcgctaacgt taatctgagg aaattatggc tgacactcgc
    21241 cctgaacgtc gctttacgcg cattgatcgt ctcccgccct acgtttttaa catcaccgct
    21301 gaactgaaaa tggctgcgcg tcggcgcggc gaagatatta tcgatttcag catgggtaac
    21361 ccggacggtg cgactccgcc gcatatcgtc gaaaaattat gtactgtggc ccagcgcccg
    21421 gacacgcatg gttactccac ttcacgcggc attccgcggt tacgtcgcgc catttcccgc
    21481 tggtatcagg atcgctacga cgttgaaatc gacccggaat cagaagccat cgtcactatt
    21541 ggttcgaaag agggcctggc gcatctgatg ctggcgacgc tggatcatgg tgacacggtg
    21601 ctggtgccga atccaagtta cccgatccat atttatggcg cggtgattgc cggggcgcag
    21661 gtacgctcag tgccgctggt ggaaggtgtc gatttcttca acgaactgga gcgagccatt
    21721 cgtgaaagtt atccgaaacc gaagatgatg atcctcggct tcccgtcaaa cccaaccgcg
    21781 caatgcgtgg aactggagtt cttcgaaaaa gtagtggcgc tggcgaaacg ctacgatgtg
    21841 ctggtggtcc atgacctggc ctatgccgat atcgtctacg atggctggaa agcgccgtca
    21901 attatgcagg taccgggtgc acgcgatgtg gcagtcgagt tctttacgct gtcgaaaagc
    21961 tacaacatgg cgggctggcg tatcggcttt atggttggca acaaaacgct ggtcagcgcc
    22021 ctggctcgta ttaaaagcta tcacgattac ggcaccttta cgccgttgca ggtggcggcg
    22081 attgcggcgc tggagggcga tcaacagtgc gtgcgcgaca ttgccgaaca gtacaaacgc
    22141 cgccgtgatg tattggttaa agggctgcat gaagcgggct ggatggttga aatgccgaag
    22201 gcttcgatgt atgtctgggc gaaaatcccg gaaccatatg cggccatggg atcgctggaa
    22261 tttgccaaga agctccttaa cgaagcgaag gtttgtgtct cgccggggat tggctttggc
    22321 gactatggtg atacgcatgt tcgctttgca ctgattgaaa accgcgaccg tattcgtcag
    22381 gccatcagag ggattaaagc gatgttccgt gccgacggtt tactacccgc cagcagcaaa
    22441 catattcacg aaaacgcgga ataaacaccg tcgtcagaaa tgcaaacagg agcctgatgg
    22501 ctcctgtttt tcattgcaca gtttgctttt agatcatcag ggcaaaagta ccggcccaaa
    22561 cgatgaccat aaaagaaatg cccataaaga aatatttcac tttacatctc tccgcgtaat
    22621 tactgttacg cctgaatgac ttcattacac ctgatacagt gagttgacac cgggcaaagc
    22681 gaccttaaaa gccctgtatt ttaccgtggg catcacccca gaattctgag ctaaactgct
    22741 cgagacggcg ctaatgtacg cctaaaactg aaacgagaca agaggcattt tcttatttac
    22801 ttttagtaac ttacaagtgt ctcatatcgg cgacagttta atttctgacg gtaataatta
    22861 gatattcagc gaaggaaaaa accttaagaa aaaagccata aaaaccatga ggttattatg
    22921 gccgatttga ggagggaaag agtaagagca gtttgttaaa tgtacaacga cgattctccc
    22981 accgggcgcg ttttaaagcg acggtggatc cagaggtact gctccggtgc gcgcatgatc
    23041 tctttctcga taatcttgtt catataggca gcggcttgat tttcatctgt cgggtagcct
    23101 tccatctctg gggtgatgaa caaacgatat ccgctgtaat ccgcttttct taccatcgtt
    23161 acggtcaaca tggctgcgcc agagagacgg gagagaacat aggtgccatt ggttgtggcg
    23221 acattttcca ccgcaaagaa cggcgcgaag gagctgcctt tacgaccata atcctgatcg
    23281 ggagcaaacc ataccgcttc acctttcttc agtgcaccga caatgccgcg cagattattt
    23341 ctgccgatca tcgctttgtt agagcgcatg cgcccacggg tctgcaccca ttccatcagc
    23401 tgattattat gtggacgata ggtagccatc attggttggc acagtcccat cacgcggccg
    23461 cccagttcca gcgacataaa atggacgccg acaaccatta cgccgcgatt ttgcatttgt
    23521 gcgcgtttaa ggttatccaa cccttcaaca tcaaaccatt tacgtacgcg actgtcgggc
    23581 cagaaccatg ccatgccggt ttctaccagc gccatgccga gtgaacgaaa gttttcggca
    23641 atcatcttct cgcgttcttc cgcagaatgc tgcgggaaac aaagttccag gtttttacgg
    23701 gcgatagatt cacgacgttt caggaatggt cgcgccattg cgccaatacg cgtgccgaga
    23761 aagcagagaa caggataagg aagctgtacc caaagccaga gtacaccaag cccaaaccat
    23821 gtgagccagt agcgaggatg tagaaactcg cgggaaaatt tgcattgtgg aaacatagtt
    23881 accttctgta gatacgaatt gttgtatcgc agaagattcc atgtcgacag cggtacactc
    23941 aattggttgg gccatcatgt acgacaatgg ttcctgtaaa gagtgtaaag actagtcagg
    24001 acataaccac gaggagcatg aatgcggtat ctgtacttta tcactcgctg actgctttca
    24061 gctactgatt attaatcgtg caaagtaaat atttttccgt acacaacagt cttgcattta
    24121 acatgctgtt ttcattgatg cttagcaaaa attgccagag gttaaggatt gtcttactat
    24181 taaagtgact gaagttacag gttttttagc gcggcaggag gttgagctat gatttattta
    24241 tggatgtttc tcgccctgtg tattgtctgc gtaagcggct acatcggtca ggtactgaac
    24301 gtggtatcgg cggtctcttc tttcttcggc atggtgatcc tcgctgcact catttattac
    24361 ttcaccatgt ggttaactgg cggtaatgaa ctggtgacgg ggatatttat gtttcttgcc
    24421 ccggcttgtg gcttgatgat tcgctttatg gtggggtatg gcaggcgata gtgggtcagg
    24481 atggctcatt tgggcaggtg ttcacatcag catcagacat gattgttcac ctgccatgtg
    24541 acagacgcta tcgcacagga tgaataagat aattcgcctt tgtcggataa gtaggcgtga
    24601 tttcttctgt gaggtttaat ggtggcgcat cttctctcat cttaaacaaa atcgcctgtg
    24661 atggctgaag ttttgtagag tgtgtggtcg tcatcgctga tgttttttca gtctctttat
    24721 agctcctctc acttacacca ccagaaatgg aaaatgcaaa aacattgcat gccataatag
    24781 agaccaataa aaataagctg aaaagtatta agtttacttt catagaaagt tatcctgaaa
    24841 atggttcact gagaacctac gatgttatgg ctgtatttac atataattaa tcaatattta
    24901 catcgatata ataaatgaca tctctttgtg gtacataaga atagttctct gcgacaggaa
    24961 gcagattcct acaattgtaa gactaaaata cttcttgcga taataactac aactgtaaga
    25021 taaccctttc aaaatgaccg ttgctctctg atttctcatt tcatgctcac ccaatatgat
    25081 ggcggcgttt tctaaaactg ttaaagaatg aggtaagtat gaaacgttta attatggcta
    25141 cgatggtcac agcaattctg gcatcttcaa ctgtatgggc tgctgataat gcaccggtag
    25201 cggcacaaca gcaaactcag caagttcagc aaacccagaa aacggcagca gcagagcgta
    25261 tctctgaaca aggtttatat gcgatgcgtg acgtgcaggt agcgcgcctg gctctgtttc
    25321 atggcgatcc agaaaaggca aaagaactga ccaatgaagc ttccgctttg ctgtcagatg
    25381 acagtaccga atgggcaaag ttcgctaagc caggtaaaaa gaccaatctc aatgatgacc
    25441 aatatattgt cattaatgca tctgtcggaa tttctgagag ttatgtagca actcccgaaa
    25501 aagaagccgc gataaaaatt gccaatgaaa aaatggcgaa aggggataaa aagggcgcaa
    25561 tggaagagct tcgcctggcc ggtgttggcg taatggaaaa ccaatatctg atgccactta
    25621 aacagactcg taatgcactt gctgatgcgc agaaactgct tgataaaaag caatactacg
    25681 aggcaaacct ggcgcttaaa ggtgcggaag atggaatcat cgtcgatagc gaagcactgt
    25741 ttgtgaacta atgctgacag gcagtatatt gaggtaaagg atttaactcc ctccagccga
    25801 caatttttta tgaccacaat acgacaggtt gtggtcataa aattatttgg cgctcgcggt
    25861 gctataaact cgcattttat attcctcaaa gtaaaccgca ataatcccgt gtcatcacaa
    25921 atcatttatt atgttatgtt ttatggtacg ctttaaaaag cgtaatcata cccttgcttg
    25981 taaatcaccg gcgcttctta cagttgtaag aataacatca caaatctcgc ggctctctct
    26041 gattaccgcg aagcaatatt tatcatattc ttcttttcgt cctcactact taccatgcag
    26101 aaaggaattg gaagtggcga tgaaatgatt ttcagcactt gatacaggaa tgtgaaaata
    26161 tcgagtgccg ctacgacgat attttgcggg ttatttatag aagatattac gccttaaata
    26221 aacgctaaat taacacatat ttaattaact aataggtatt gctatgtcaa ctccacttca
    26281 aggaattaaa gttctcgatt tcaccggtgt gcaatctggc ccatcttgta ctcaaatgct
    26341 ggcctggttt ggcgctgacg ttattaaaat tgaacgtccc ggcgttggtg acgtaacgcg
    26401 tcaccagctg cgagatattc ctgatatcga tgcgctttac ttcaccatgc ttaacagtaa
    26461 caaacgttct attgagttaa ataccaaaac agcggaaggc aaagaggtaa tggaaaagct
    26521 gatccgcgaa gctgatatct tagtcgagaa ctttcatcca ggggccattg atcacatggg
    26581 cttcacctgg gagcatattc aagaaatcaa tccacgtctg atttttggtt cgatcaaagg
    26641 gtttgatgag tgttcgcctt atgtgaatgt aaaagcctat gaaaacgttg ctcaggcagc
    26701 gggtggcgcg gcatccacta cgggtttttg ggatggtccg ccgctggtaa gcgctgcagc
    26761 gttgggtgac agcaacaccg gaatgcattt gctgatcggt ttacttgctg ctttgctgca
    26821 tcgcgaaaaa acggggcgtg ggcaacgagt caccatgtca atgcaggatg ccgtattgaa
    26881 cctttgccgc gtgaaattac gtgaccagca gcgtctcgat aaattgggtt atctggaaga
    26941 atacccgcag tatccgaatg gtacatttgg tgatgcagtt ccccgcggtg gtaatgcagg
    27001 tggtggcggt cagcctggct ggatcctgaa atgtaaaggc tgggaaaccg atcctaacgc
    27061 ctatatttat ttcactattc aggagcaaaa ctgggaaaac acctgtaaag ccatcggcaa
    27121 accagaatgg attaccgatc cggcatacag tacagcccat gcacgacagc cacatatttt
    27181 cgatattttt gctgaaatcg aaaaatacac tgtcactatt gataaacatg aagcggtggc
    27241 ctatttgact cagtttgata ttccttgtgc accggtttta agtatgaaag aaatttcact
    27301 tgatccctct ttgcgccaaa gtggcagtgt tgttgaagtg gaacaaccgt tgcgtggaaa
    27361 atatctgacc gttggttgtc caatgaaatt ctctgccttt acgccggata ttaaagctgc
    27421 gccgctatta ggtgaacata ccgctgctgt attgcaggag ctgggttata gcgacgatga
    27481 aattgctgca atgaagcaaa accacgccat ctgatattaa cgggggcttc tggctcccaa
    27541 tttataaaaa tttcgaggtt attaatcatg tcagatcaac ttcaaatgac agatggtatg
    27601 catatcatcg ttgaagcatt aaaacagaat aatattgaca ctatttatgg tgttgtaggt
    27661 attcctgtga cggatatggc acgccatgcc caggcggaag gcattcgtta tattggtttt
    27721 cgtcatgagc agtcggcagg ctatgccgct gcggcaagcg gttttcttac ccaaaaaccg
    27781 gggatctgcc tgacagtttc tgcgccagga ttcctcaatg gtttgaccgc attggccaac
    27841 gcaacggtaa atggttttcc gatgatcatg attagcggct ccagcgaccg cgcgatcgtc
    27901 gacctacagc aaggtgatta tgaagagctg gaccaaatga atgcggcaaa accgtatgcc
    27961 aaagcagcat ttcgcgttaa tcagccgcag gatcttggca ttgcattggc acgcgctatc
    28021 cgggtctctg tatcgggtcg ccctggcgga gtttatcttg atttgccagc aaatgtcctg
    28081 gccgcgacga tggaaaaaga cgaagcgtta accacgattg ttaaagttga aaatccgtcg
    28141 ccagcattat tgccatgccc gaagtcagtc actagcgcaa tttcgctttt agcaaaagct
    28201 gaacggccat taattatcct tggcaaaggc gcggcgtatt cacaagctga tgaacagctt
    28261 cgtgaattta ttgaaagtgc tcagattcca ttcctgccaa tgtctatggc gaaagggatc
    28321 cttgaagata cgcatccact ttctgcggca gctgcgcgtt cgtttgccct ggcaaatgct
    28381 gacgttgtca tgcttgttgg tgcacgactg aattggttat tggcacacgg taaaaaagga
    28441 tgggcggcag atacacagtt tattcaactg gatattgaac cgcaggaaat tgacagcaac
    28501 cgccccattg ctgtgccagt cgttggtgat attgcatcca gtatgcaagg tatgctggca
    28561 gaactgaaac aaaacacatt tacgactcca ctggtatggc gcgatatttt aaatatccac
    28621 aagcagcaaa atgcacaaaa aatgcatgaa aaattaagta cagatactca accattaaat
    28681 tactttaatg cattaagtgc tgtgcgcgac gtattgcgcg agaaccagga tatttattta
    28741 gttaatgaag gtgcaaatac cctggataat gcacgaaata ttattgatat gtataaacca
    28801 cgtcgtcgtc tggattgtgg tacctggggt gtcatgggca tcggtatggg ctatgccatc
    28861 ggtgctagcg tgacttctgg ttctccggtt gtcgccattg aaggtgatag tgcttttggt
    28921 ttcagtgg
//
LOCUS       DACAPB010000039        28918 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.039, whole genome shotgun sequence.
ACCESSION   DACAPB010000039 DACAPB010000000
VERSION     DACAPB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.039"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..374)
                     /gene="flgC"
                     /locus_tag="IT904_001555"
     CDS             complement(<1..374)
                     /gene="flgC"
                     /locus_tag="IT904_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAP3191352.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKS"
     gene            complement(378..794)
                     /gene="flgB"
                     /locus_tag="IT904_001556"
     CDS             complement(378..794)
                     /gene="flgB"
                     /locus_tag="IT904_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAP3191353.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMPRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            950..1609
                     /gene="flgA"
                     /locus_tag="IT904_001557"
     CDS             950..1609
                     /gene="flgA"
                     /locus_tag="IT904_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAP3191354.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            1685..1978
                     /gene="flgM"
                     /locus_tag="IT904_001558"
     CDS             1685..1978
                     /gene="flgM"
                     /locus_tag="IT904_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAP3191355.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            1983..2399
                     /gene="flgN"
                     /locus_tag="IT904_001559"
     CDS             1983..2399
                     /gene="flgN"
                     /locus_tag="IT904_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAP3191356.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(2439..3974)
                     /gene="murJ"
                     /locus_tag="IT904_001560"
     CDS             complement(2439..3974)
                     /gene="murJ"
                     /locus_tag="IT904_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAP3191357.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(4084..5007)
                     /locus_tag="IT904_001561"
     CDS             complement(4084..5007)
                     /locus_tag="IT904_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP3191358.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASMDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(5009..5656)
                     /locus_tag="IT904_001562"
     CDS             complement(5009..5656)
                     /locus_tag="IT904_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAP3191359.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(5667..6251)
                     /gene="rimJ"
                     /locus_tag="IT904_001563"
     CDS             complement(5667..6251)
                     /gene="rimJ"
                     /locus_tag="IT904_001563"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAP3191360.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            6487..7695
                     /gene="mdtH"
                     /locus_tag="IT904_001564"
     CDS             6487..7695
                     /gene="mdtH"
                     /locus_tag="IT904_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAP3191361.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFNLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            7759..8406
                     /gene="grxB"
                     /locus_tag="IT904_001565"
     CDS             7759..8406
                     /gene="grxB"
                     /locus_tag="IT904_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAP3191362.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDYAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            8540..9100
                     /locus_tag="IT904_001566"
     CDS             8540..9100
                     /locus_tag="IT904_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP3191363.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            9206..10252
                     /gene="pyrC"
                     /locus_tag="IT904_001567"
     CDS             9206..10252
                     /gene="pyrC"
                     /locus_tag="IT904_001567"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP3191364.1"
                     /translation="MTAPYQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            10326..10571
                     /gene="dinI"
                     /locus_tag="IT904_001568"
     CDS             10326..10571
                     /gene="dinI"
                     /locus_tag="IT904_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAP3191365.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            10861..11115
                     /gene="bssS"
                     /locus_tag="IT904_001569"
     CDS             10861..11115
                     /gene="bssS"
                     /locus_tag="IT904_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAP3191366.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            11230..12348
                     /gene="solA"
                     /locus_tag="IT904_001570"
     CDS             11230..12348
                     /gene="solA"
                     /locus_tag="IT904_001570"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAP3191367.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            12396..12509
                     /locus_tag="IT904_001571"
     CDS             12396..12509
                     /locus_tag="IT904_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAP3191368.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            12770..13336
                     /locus_tag="IT904_001572"
     CDS             12770..13336
                     /locus_tag="IT904_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAP3191369.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            13340..13915
                     /locus_tag="IT904_001573"
     CDS             13340..13915
                     /locus_tag="IT904_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAP3191370.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAVDYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQDVDLIISVEGVQQK"
     gene            complement(13957..15009)
                     /locus_tag="IT904_001574"
     CDS             complement(13957..15009)
                     /locus_tag="IT904_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP3191371.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDSALDGLRLNIALDDD
                     RKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            15234..16154
                     /locus_tag="IT904_001575"
     CDS             15234..16154
                     /locus_tag="IT904_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAP3191372.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            16326..17552
                     /gene="mdtG"
                     /locus_tag="IT904_001576"
     CDS             16326..17552
                     /gene="mdtG"
                     /locus_tag="IT904_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HAP3191373.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            17635..18009
                     /locus_tag="IT904_001577"
     CDS             17635..18009
                     /locus_tag="IT904_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAP3191374.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(18010..18237)
                     /locus_tag="IT904_001578"
     CDS             complement(18010..18237)
                     /locus_tag="IT904_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP3191375.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(18410..20953)
                     /gene="mdoH"
                     /locus_tag="IT904_001579"
     CDS             complement(18410..20953)
                     /gene="mdoH"
                     /locus_tag="IT904_001579"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAP3191376.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(20946..22481)
                     /gene="mdoG"
                     /locus_tag="IT904_001580"
     CDS             complement(20946..22481)
                     /gene="mdoG"
                     /locus_tag="IT904_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAP3191377.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            22875..24032
                     /gene="mdoC"
                     /locus_tag="IT904_001581"
     CDS             22875..24032
                     /gene="mdoC"
                     /locus_tag="IT904_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAP3191378.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(24040..25521)
                     /locus_tag="IT904_001582"
     CDS             complement(24040..25521)
                     /locus_tag="IT904_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAP3191379.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(25463..25996)
                     /gene="ymdB"
                     /locus_tag="IT904_001583"
     CDS             complement(25463..25996)
                     /gene="ymdB"
                     /locus_tag="IT904_001583"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAP3191380.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSFTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(26091..26402)
                     /locus_tag="IT904_001584"
     CDS             complement(26091..26402)
                     /locus_tag="IT904_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3191381.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTVQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(26523..26855)
                     /gene="csgC"
                     /locus_tag="IT904_001585"
     CDS             complement(26523..26855)
                     /gene="csgC"
                     /locus_tag="IT904_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAP3191382.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTRLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(26914..27369)
                     /gene="csgA"
                     /locus_tag="IT904_001586"
     CDS             complement(26914..27369)
                     /gene="csgA"
                     /locus_tag="IT904_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAP3191383.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNFE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(27410..27865)
                     /gene="csgB"
                     /locus_tag="IT904_001587"
     CDS             complement(27410..27865)
                     /gene="csgB"
                     /locus_tag="IT904_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAP3191384.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            28620..>28918
                     /locus_tag="IT904_001588"
     CDS             28620..>28918
                     /locus_tag="IT904_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAP3191385.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIE"
BASE COUNT         7319 a         7402 c         6938 g         7259 t
ORIGIN      
        1 atgcttttca ccgtgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg
       61 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatct
      121 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
      181 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
      241 tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg
      301 gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
      361 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
      421 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
      481 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
      541 agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga ggtcatcgtc
      601 agtgcaacca cactggttgc atcccgtcca cgtggcatga cttttttaag ttcactggca
      661 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
      721 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
      781 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
      841 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatgac
      901 cattgcggac aggtacaatt cacgttgtag aaatggctgg ggggcgaaaa tgctggcaat
      961 aaaacgtagc gtggcgatca ttgcgatact gttcagtccg ttaagtgcgg cgagcaatct
     1021 cacatcgcaa ttgcaaacct tttttagcgc ccagctcgcg ggggtaagtg atgaggttcg
     1081 tgtttctatt cgtacagcgc ccaatctact accgccgtgc gagcagccat tgctttcgat
     1141 gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa
     1201 acgatattta caggttaatg tacaggccac aggaaattat gtggttgccg cgatgcccat
     1261 tgcgcgggga ggaaagctgg aagctggcaa tgtcaaactg aaacgcggac ggctggatac
     1321 cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt gatgccatta gcctgcgcga
     1381 tctatcaccc gatcaaccta tccagttaac ccagtttcgc caggcatggc gggtaaaagc
     1441 gggacaacgc gtcaacgtga tcgccagcgg tgatgggttt agcgccaacg cagaaggtca
     1501 ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg cgcatggtat cgggacaggt
     1561 agtcagcggc gttgttgatg cagatgggaa tattcttata aacctgtaag ctgttaaaga
     1621 ttacccgtcc cttgccgata aataagcaac acatgataaa agcgccctca atgaggaata
     1681 aaccatgagt attgatcgca cttcgcctct gaagcctgta agcaccgttc aaccgcgcga
     1741 aaccactgac gcgccggtaa cgaacagccg ggcggcaaaa acaaccgcct ccaccagcac
     1801 cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa
     1861 tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac ggtgaactaa aaatggacac
     1921 cggcaaaatt gccgatgcgc tgatcaacga agctcagcaa gatttgcaga gtaactgatc
     1981 gtatgacacg tcttgcagag atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa
     2041 cggtaatgga tcaagagcag caacatctct ctatggggca gatcaacggc agccagttgc
     2101 aatggattac agaacaaaaa agctcactgc tggcgacgct ggattacctc gaacagttac
     2161 gcaagaaaga acccaataca gcaaatagcg ttgatattag tcaacgctgg caggaaatta
     2221 ctgtgaaaac gcagcaacta cgccaaatga atcaacataa cggctggtta ctggaaggac
     2281 agattgagcg caatcaacag gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat
     2341 atggggcgaa cggtcagacc tcaacaaccc atcgcggcgg taaaaagatt tcgatctgaa
     2401 tattatctcc ggcctgcact gcaggccgga atgcattgtt acaccgtccg gcgggcaaat
     2461 tctttaactt tgaagcccag taccgccagt gcagcgaagt acgcggcaat ccccgccagc
     2521 acgaccgcca ttaaacgcag taagcgccag ggcatggtac ccaatgacca ctccggcatg
     2581 atatgtaaca tacctaaaag cacgccagac atcaccagta ccgccaccac cagacgcaac
     2641 agaaacgcca tccagccggg ttgcggagta aagattttct gcttacgcaa ctgccagtaa
     2701 agcagcgaag cattcagaca cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc
     2761 aacggaccaa taaacgccag gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt
     2821 ttcactggcg ttttaatgtc ctggcgggaa taaaagccag gagccaacac tttcactaca
     2881 atcaggccaa tcaaacccac cgagtaggca attaacgccc gctgggtcat cagcgcatca
     2941 aacgcggtaa atttaccgta ctggaacagc gaaacggtta acggacctga aagaatgccc
     3001 aacgcaaccg cactcggcag cgccaacagg aaacaaagac gcaaccccca gtccatcaaa
     3061 cggttgtatt catcatgatt gccactggca aaacttttcg acagcgatgg cagcaaaatg
     3121 gtgccaagcg ccacgcccag cacaccggag ggaaactcca ttaagcggtc ggcgtaatac
     3181 atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa tggtgttgat gattaaggag
     3241 atctggctca cagagacgcc aaggatcgcc ggtcccatct gtttcaccac gcgcattgct
     3301 ccggcatcgt ggaagttaat gcgcggcagg accagcatgc cgatcttctt caggtgcggt
     3361 agctgataca ccaactgcag gatgccgccg accgtaacag cccacgccag cgccagcacc
     3421 ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt
     3481 gttggcgcaa acgccggaat cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc
     3541 gacgccaggg agataagcaa gatataggga aaggtaatct ttagtagctg gctggtcagg
     3601 gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg
     3661 gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa
     3721 gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt tatattccgc cagaatcggt
     3781 acaaatgcct gggaaaatgc cccttcggca aagatacggc gtaacaagtt aggaagttta
     3841 aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa agattctggc gacaattgcg
     3901 tctcgtgcga agccaagcac acgcgaaaac atggtcatcg agctgacggc ggccagcgat
     3961 tttaataaat tcatcggtgt tctaatccag acccacgggc aaaacgcctg caaggcaggc
     4021 gctgaaagag gcgaatagtc tacctggatt atggtgaatt gctaccgcca gatgttacag
     4081 ggtttattca ctcatcgcat cgcgccagat cttgtcaacg atacgttgcg cccgcactgc
     4141 ctgttcgccg gcggtttgcg gaactgtctg attttgcaca cattcaatga agtgccgcgc
     4201 acagccgaca aatccacgtt gctcaagagt gctctgccaa ccaggaatcg gtttattcac
     4261 tacgccctgc ccacgctcct cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc
     4321 gtcagtcacg gcctgcacgg tttcacgctg gcttccggca cggcgatgca tacaggtggt
     4381 gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt
     4441 agtcagtagc gtaccgccat ccatagaggc tttgccgccc gacaaccaca gcgcggtatc
     4501 caccacatgc agataatcat ccagcaacgt gaaataaaga tcgtgtggcc cgacgctatt
     4561 gctacgatgt ttatccattc ttagcgaggc tgcggtggcg agttgcgttt ttaactcacc
     4621 gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac gtcaattttt tccgcgccgc
     4681 cagttccacc agccgttcag catcgcgcag attttctgcc agcggtttat cgacacagac
     4741 atgtaccccc gcattgagta acgtactaac cacgtcaaag tggctggcgg tgctggaatg
     4801 cacaaaaacc gcatcgcaac tggcggcaag gctggataac gaatcggcat aaggaatgcg
     4861 ccagctttca caaattggca gggctttcgc gcgcgtaggc gaccaggctc cttgtaacat
     4921 ccagtcagac gctgccgcca gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc
     4981 cactacgccg atacgtaatt ttttcacttt aatctcccag atgggccagc aacgaatcaa
     5041 gacgctgttt cagttctgcc acttcgattt ccagagcttc gacgcgggcc tgtaaatcac
     5101 cgtcaaccgc gttcgacata tccgttaccg ccggctgatc ttcaacctca ccactgaaaa
     5161 gatgcatgta gcggctttca cgtttacccg gttcgcgggc cagacgcacc acaaaaggac
     5221 catcttcgcg atttgccagt tgttccagcg tcgactcaac ttccgccata tcgctgaatt
     5281 catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca
     5341 acgtggtgat taacgccact tctgcggcgc tcagtttcag atcgccaaat tctgaattac
     5401 aaaaacgttg ttcatatttg gtgacccgat taccaaagcc actcaccgtg cgcagataat
     5461 ggcgtttgac cagattatcc agttgctcct gcacctcgga ttcactcaga ttcatgaccg
     5521 gttcacggtt cgttttctga ttacaggccg tgactacacc attgactgag agcgggtatt
     5581 gctccggcgt ggtcacctgc ttttccagca agcaaccaat cacacgagct tccagggcag
     5641 taagttgata tttcatgaga ttctccttag cggccgggcg tccagtctgg ggtagttaat
     5701 gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca gatagtcttt cgcatagcct
     5761 tctttttcaa aacccagtcg tgccagtaaa tcaccgctgc gtttattgtg cggcatataa
     5821 ttagccataa tgcgatgaat atgttgggtg cgctgcatat aacgaatggc tgcggtcagg
     5881 gcttcaaaca tgagtccttt gccctgccat ttttgcccaa tcgaataacc gagatagcag
     5941 gcatgaaaag agccgcgaac aacattggaa aaattggcaa cgccaataat ctctttttcg
     6001 tccgggtcga ataagccaaa gtagaacgct gaaccttgtt tatgaaattc gttaatcatc
     6061 cccagcctgg cctgccagcc tgatggataa cagtggcttt cgtcgcgcac tggctcccag
     6121 ggcttgagga aatggcgatt ctctgcgtaa taatccgcaa gacgccaggc atcacgatca
     6181 tgcaccagac gcacgaccag tcggtctgtg gttaagcgca cttttggcac gttactgcga
     6241 tagccaaaca ttctatacct actccttccc gtaacgtctt taatacgctg taagtattac
     6301 tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc cattgtctgc attattcaca
     6361 tatttgataa aaactctcta tcaaagcgac gttttgataa aaaaatattg tccccgaagt
     6421 gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc tcatctttta tttcccggga
     6481 ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta aatatttcct gctcatcgat
     6541 aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt
     6601 gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc tcggtctacg ccagtttatt
     6661 cagcaaggtc tgggtatttt cggcggcgca attgccgacc gctttggtgc caaaccgatg
     6721 attgttaccg gtatgctgat gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa
     6781 ccgtggctat tgtggttttc atgcctgctc tcgggactcg gcggcacgtt gtttgatccg
     6841 ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac aacgtggtcg ttttttctcg
     6901 ctgttgatga tgcaggacag tgccggtgcg gtcattggcg cattgttggg gagctggctg
     6961 ttgcaatacg actttcgcct ggtctgcgcc acaggggccg ttttgtttgt gctgtgtgcg
     7021 gcgttcaatg cgtggttgtt accggcatgg aaactctcca ccgtacgcac gcccgttcgc
     7081 gaaggcatga cccgcgtgat gcgcgacaag cgttttgtca cctatgttct gacgctggcg
     7141 ggttactaca tgctggctgt acaagtgatg ctgatgctgc caattatggt caacgacgtg
     7201 gctggcgcgc cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg
     7261 ttgctctacc ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttgatg
     7321 gctgggctgt tgataatgtc attaagcatg atgccggtgg gcatggtcag cggcctgcaa
     7381 caacttttca acctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt
     7441 gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg gcagctatat ggggtttagc
     7501 cgtctgggtc tggcgattgg tggcgctatt ggttatatcg gtggcggctg gctgtttgac
     7561 ctgggcaaat cggcgcacca gccagagctt ccgtggatga tgctgggaat tattggcatc
     7621 ttcactttcc ttgcgctggg ttggcagttt agtcaaaaac gcgccgcgcg tcgtttgctg
     7681 gaacgcgacg cctgatttga tactcccccc tgactcctcc atactgaaag tgaactgaca
     7741 gccaaatgga ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa
     7801 gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac
     7861 tacgcagaaa cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat
     7921 gacagccgct atatgccaga aagcatggat atcgttcact atgtcgataa actcgacggc
     7981 aaaccgttac tgaccggcaa acgttcccct gccattgaag agtggctgcg caaagtcaat
     8041 ggctacgcca acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct
     8101 actcccgccg cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc
     8161 gacctgctgg cccactctga cggtctgatt aagaatatca gcgatgattt gcgtgcgctg
     8221 gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac tttcggaaga tgatattcag
     8281 ctatttccgc tactgcgtaa tctgacgctg gtagccggaa tcaactggcc aagccgcgtt
     8341 gctgattacc gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg
     8401 atttaatcct gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt
     8461 tctgctggcg agtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga
     8521 tttcatgtcg aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg
     8581 cctgctcgtt ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca
     8641 gtcgcttgcg aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc
     8701 ccatattgtt ctgacaaact tgaccagcca aattggtcgc gaagagccga ataaggttac
     8761 cctaaccgga gacgccaatc tggacatgaa ctccctgttc ggtagtcaga aagcgaccat
     8821 gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa
     8881 agagatggaa gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac
     8941 gttgcttccc tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt
     9001 cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa aaactggcga aaggcattga
     9061 agtgaagcca ggcgaaattg tcattccgtt tacggattaa tgacgagggc gcattcgcgc
     9121 cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat
     9181 cccttcagcc ggagcataga gattaatgac tgcaccatac caggtattaa agatccgccg
     9241 cccagacgac tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcgtgccgta
     9301 taccagcgaa atttatggac gggctatcgt aatgcccaat ctggctccgc ccgtgaccac
     9361 cgttgaggct gccgtggcgt atcgccagcg tattcttgat gccgtacctg ccgggcacga
     9421 tttcacccca ttgatgacct gttatttaac agattcgctg gatcctaatg agctggagcg
     9481 cggatttaac gaaggcgtgt tcaccgctgc aaaactttac ccggcaaacg caaccactaa
     9541 ctccagccac ggcgttacgt cagttgacgc aatcatgccg gtacttgagc gcatggaaaa
     9601 aatcggtatg ccgctactgg tgcatggtga agtgacacat gcagatatcg acatttttga
     9661 tcgtgaagcg cgctttatag aaagcgtgat ggaaccgctg cgccagcgcc tgactgcact
     9721 gaaagtcgtt tttgaacaca tcaccaccaa agacgcagcc gactatgtcc gtgacggaaa
     9781 tgaacggctg gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct
     9841 ggttggtggc gtgcgtccgc acctgtattg tttacccatc ctcaaacgca atattcacca
     9901 acaggcattg cgtgaactgg tcgccagcgg ttttaatcga gtattcctcg gtacggattc
     9961 tgcgccacat gcacgtcatc gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc
    10021 cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag atgaatgctc tgcagcactt
    10081 tgaagcattc tgttctgtaa acggcccgca gttctatggc ttgccggtca acgacacatt
    10141 catcgaactg gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac
    10201 cctggtgcca ttcctcgccg gggaaacggt acgctggtcc gttaaacaat aaaaatcgta
    10261 gcttcctgtt gtcattaggt tattttacct gtataaataa ccagtatatt caacaggggg
    10321 ctattatgcg aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg
    10381 acgccctggc tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc
    10441 acgtatcggt acgttatgcc gcagcgaata atttatcggt tattggcgca acaaaagaag
    10501 ataaacagcg cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg
    10561 tcagcgaata atatgcagtg atttttgttt ttgccgggtc gccccggctt ttttatttta
    10621 cttacgaatt ttgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa
    10681 aaaaatatga atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc
    10741 tgttcccccc tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc
    10801 agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg
    10861 atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc
    10921 gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag
    10981 caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt
    11041 atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc ggtaaacgag
    11101 tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta
    11161 ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga
    11221 gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc
    11281 gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg
    11341 catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc
    11401 gaaaagtatg tcccactggt actccgcgcg caaacactgt gggatgagct ctcccgccac
    11461 aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc ggctgactcc
    11521 gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt cgaaaagctc
    11581 gacgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggc
    11641 ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa
    11701 ctggcgaagg aagcgggctg tgcgcagctg ttcaactgcc cggtcaccgc aattcgtcat
    11761 gacgatgatg gcgtaactat tgaaacggct gacggagagt atcaggcgaa aaaagcgatt
    11821 gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca gcccgtacgc
    11881 aaagtatttg cctggtatca ggccgatggt cgctatagcg tgaagaataa attcccggcg
    11941 tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg
    12001 ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg
    12061 tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa tgtcttgccg
    12121 ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat
    12181 tttattatcg ataccctacc cggtcacgat aatacactgc tcattaccgg cctgagcggg
    12241 cacggtttta aatttgcgtc tgttttaggg gaaatagctg ccgattttgc gcaagacaaa
    12301 aaaagcgatt ttgatttaac gccattcagg ctttcccgct tccaataatc atcattacgg
    12361 cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg cactatctca
    12421 tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta
    12481 tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt
    12541 taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt ctacagcact
    12601 caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca ttattaattc
    12661 aaaattattg aaataaaaat aaatatcatt caatttgtta tttttgtgtt gcaattcatt
    12721 ctccgacacg ccattttctt tctctcgcaa attcgttttg gatgcactta tgtcattcac
    12781 aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat
    12841 tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt acgatggctg
    12901 gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt
    12961 tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc
    13021 catgactcgt cttgccgcaa aagctggaca tctcgcattg tacctattgc tgttcgcaat
    13081 cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg
    13141 gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag gcagactttg ccggtgccct
    13201 gcatttttgg ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat ttatggccct
    13261 gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc
    13321 tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat
    13381 gttctctgcc ggttcagcgg ttgccgtcga ttacaaaatt gacaaagaag gtcagcacgc
    13441 ctttgttaat ttccgcatcc agcaccttgg ctatagctgg ttatacggca cctttaaaga
    13501 tttcgacggt acttttacct ttgacgaaaa aaatccggct gccgataaag tgaatgtgac
    13561 aattaacacc accagcgtcg atactaatca cgccgaacgc gataaacatc ttcgcagtgc
    13621 agatttcctc aataccgcaa aatatccaca ggcaacattc acctccacca gcgtgaagaa
    13681 agacggtgac gaactggata ttaccggcga tctgacactg aatggcgtaa ccaaacccgt
    13741 cacgctggaa gcgaaattaa ttggtcaggg cgacgacccg tggggcggta aacgtgcagg
    13801 ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat atcaagacag atttaggtcc
    13861 agcttctcag gatgtggatc tgattatttc agtggaaggc gtacagcaga agtaagcgtg
    13921 aaatgttgcc ggatggcacg catccggcaa tgatatttat tctgttggat caggaatgcc
    13981 cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc
    14041 tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact
    14101 acaacaacct ttgtattttt ccgcgcatac tggacactga ataaacagca ggtggcagcc
    14161 atcattttta cagttggtat ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat
    14221 aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa
    14281 acgcaccggc aagccctgct cgcgcgcctt acgggcgtat tcaataattc cgccctcgat
    14341 atgccagact ttgttgaatc cgttatgttt catccaggca ctggcctttt cacaacgaat
    14401 gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc
    14461 ttttggcagc tgctcacgga aggtatctgc cggaatttcg agcgcgtttt caaagtgccc
    14521 cacttcatac tcatagtggt tacgcatgtc gataaacagt gcatcgggat cgtcaagcat
    14581 ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa agtgaggatc
    14641 gtcaataccg tcggcaacga tacgatcgcg gaccttcatg cgcagtaccc agaaggattt
    14701 cctgtcatca tccaacgcga tattcaggcg taagccatct aaagccgagt cgaaggcata
    14761 gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg cgttaatgcc
    14821 ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagct gatataaagc
    14881 gtcacgggtc gccttaggat cggcgatgtg gaaatacttg taaaacgaaa tggtggttcg
    14941 cggttcgctc tcagccaaca ttttggcttt tagcgcgtcg ttggaaatgc ggttgtgtaa
    15001 cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg catgatatag
    15061 caattatcga taattaacat ccacacattt tacgctacat ttgcgcatta aaaattattt
    15121 gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg ttgcgggcga
    15181 aaaaatgcga caatacatac aattgcccga ataggttgaa aaacaggatt gatatgacga
    15241 atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc tggttgggta
    15301 ttggcgtact ttggttagtc gtgcaattgc cctacccggt tatctaccgc ctcggttgtg
    15361 gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg catcgcaacc
    15421 tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg gtgaagaatt
    15481 tcgaatccgt tggcatgggc ctgatggaaa ccggcatggc gtggttctgg ccggaccgcc
    15541 gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg caggcgcaaa
    15601 aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagctgggt gcgcggcagt
    15661 ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca ctgattgact
    15721 ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc aaagatttaa
    15781 aaggcatgat taaagccctg aaaaaaggcg aagtggtctg gtacgcaccg gatcatgatt
    15841 acggcccgcg ctcaagcgtt ttcgtcccgt tgtttgccgt tgagcaggct gcgaccacga
    15901 ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc gttccacgcc
    15961 gtaagccaga tggcaaaggg tatcaattga ttatgctgcc gccagagtgt tctccgccac
    16021 tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa tgcatcatga
    16081 tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgcccg gaaggcgttc
    16141 cttcacgcta ttaaatctcc catgccggat gcttcagaat ggcatccggc attaccacag
    16201 caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga agtcgggcgc
    16261 ataattagtg tgcttatctt ttcttcttat gttcaccgcg cctggcgcac caacagcgga
    16321 ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac ctgatcgtcg
    16381 cctggctagg ctgttttctt accggggccg ccttcagtct ggtaatgccc ttcttacccc
    16441 tctacgttga gcagcttggc gttaccggtc actccgccct gaatatgtgg tccggtattg
    16501 tcttcagcat tacattttta ttttcggcca tcgcctcacc gttttggggt ggactcgccg
    16561 accgtaaagg ccgaaaactc atgctattac gctctgctct cggcatgggc atcgtaatgg
    16621 tgttgatggg actggcacaa aatatctggc agtttttgat cctacgggcg cttcttgggt
    16681 tacttggcgg atttgtcccc aacgctaatg ctcttatcgc cacacaagta ccgcgtaata
    16741 aaagcggctg ggcgctgggt acgctctcca caggcggcgt tagtggtgcg ttgctcggcc
    16801 caatggctgg cggcctgctc gccgatagct acggcttacg tccggtattc tttattaccg
    16861 ccagtgtgct catactctgc tttttcgtca ccctgttttg catcagagaa aaattccagc
    16921 cggtcagcaa aaaagagatg ctgcacatgc gggaagtggt gacatcactt aaaaacccga
    16981 aactggtact cagcctgttt gtcactacgt taatcatcca ggtggcgacg ggctcaattg
    17041 cccccattct gacgctgtat gtccgcgaac tggcgggtaa cgtcagtaac gtcgccttta
    17101 tcagtggcat gatcgcctcg gtgccaggcg tggcggctct gctaagtgca ccacgactcg
    17161 gcaaacttgg cgatcgaatc ggacccgaaa agatcctgat tacagcgctg atcttttctg
    17221 tactgctgtt gatcccaatg tcttacgttc agacgccatt gcaacttggg attttacgtt
    17281 ttttgctcgg tgccgccgat ggtgcactac tccccgccgt acagacactg ttggtttaca
    17341 actcgagcaa ccagatcgcc gggcgtatct tcagctataa ccaatcgttt cgtgatattg
    17401 gcaacgttac cggaccattg atgggagcag cgatttcagc gaactacggt ttcagagcgg
    17461 tatttctcgt caccgctggc gtagtgttat tcaacgcagt ctattcatgg aacagtctac
    17521 gtcgtcgtcg aataccccag gtatcgaact gatttttcgc ctttcatact tgcaaaagcg
    17581 gagaatcagc tatccttttc cctgaaacct catcaactca aagggagaat cgtgatgacc
    17641 atgtacgcaa cgcttgaaga agccattgac gctgcacgcg aagaatttct tgcagacaac
    17701 cccggcatcg acgccgaaga tgcgaatgtg caacagttca atgcccaaaa atacgttttg
    17761 caggacggcg acatcatgtg gcaagttgag ttttttgccg acgaagggga agaaggtgaa
    17821 tgtttaccta tgcttagcgg tgaagccgcg caaagtgttt ttgatggcga ctatgatgag
    17881 atagagatac gccaggagtg gcaggaagag aatacattac atgaatggga cgagggggaa
    17941 tttcagcttg agccaccgct ggataccgag gaaggacgcg cagcagctga tgagtgggat
    18001 gaacgttaat cactcatacg ggccatgatg aatgtcgatc ggcagcagta aagtatcaaa
    18061 aaccagggag aatggcagat caaggattgt gacataacgc caggcggagt cacgcacatc
    18121 ccattgcaca ccgggataat attggttgcc atgcccctgc cccggtatag tgcgactaat
    18181 aatgctgcca cagccactca gcaaggtcac catgatgctc accacaatta aacgcatttg
    18241 atacctcttc cgtaaaaaaa taccggcata acgccggtat ttttattggc tcgttgtttt
    18301 tgccggatgc ggcgtgaacg ccttacccaa cctacaaaat cttgcaaaat caataaattg
    18361 caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt tattgcgaag
    18421 ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa taactcaccc
    18481 atgaagaata tctctccggg gaattccaga cgcggaaatg cagacgggcc atcgtcaccg
    18541 gatcgcttag cagcaccagg cgacgatcgc gattcagctt ctctggcgtc tcgttaagcg
    18601 cctgttcaac gtggcggtca cgggcgattt ccagcacctt actggcgcgg tgacgcgcgg
    18661 tcgccattgc ggttgccaga gcgttaaatg acgggttaaa caccgcgtgc ataaaaccat
    18721 catcaaggga gcgttgacga ttcatctcaa ggaagcgatc ggtatcaacc agcacctgcg
    18781 gcggtgaata ctcttccggg atcaggaaca gtttccagcg tttggttcgc agaccaacgg
    18841 tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaag acaatcggtg
    18901 ccagccagaa caggaaacgc agatccaacc acgccatacc aacagcccac actaacccta
    18961 acagcagctg tgagccgtgg cgtttgaacg cttcacccca ggaagtggag tcatcatcac
    19021 gctgcggtga attccacaca acttcccagc caaggaacgc gctgacgacg aagaccgtat
    19081 ggaacagcat gcgtaccgga gccagcagca cggaaaacag cacttccagc agcaacgata
    19141 atgtaacgcg ccagaatccg ccgtattctt tcgttccttt gcaccagata agcaaaatgc
    19201 tcaataactt cggcaggaac aacagcacca tagtcgaagc aaaaagtgca atcgccagct
    19261 caggacgcca ctgcggccac accgggaaca actgccgtgg ttgcaggaag tattgcggtt
    19321 cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata aaccacagcg
    19381 gagcggagag ataagacatc acgcccgtca ggaacaccgc acggtgaacc gggtgcatac
    19441 ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg cggtcacgtt
    19501 ttagctcatc aagcaagtta ggcggcaatt cttcataaga acccgggaga tcgtaagcaa
    19561 tccagacccc ccaacctgca cggcgcatca acgccgcttc cacgaagtca tgtgacagga
    19621 ttgaaccggc aaaggaacct tcgcccggca gcggagccag tgcgcagtgc tcgataaacg
    19681 gtttcacgcg gataatcgcg ttatgtcccc agtagtgcga ctcgccaagt tgccagaagt
    19741 gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc gaactgctga cagcgcgcat
    19801 acagcgtatc cataccggac gctttcggcg acgactgaat gatcccggcg ttcgggttgg
    19861 cttccatcag gcgcaccagc ccgcacaaac aatcaccggt cattaccgag tcagcatcca
    19921 gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg atattaccgc
    19981 ttttacgctt cacgcggcga cggcggcggc gatagaaaat ctgaccttcg ccaccgactt
    20041 cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg ttataactgt
    20101 cactaagaat gtagacatca aagtgtttgg cattcccggt ggcttttact gattcccacg
    20161 ttgcacgcag gccagcaaaa acacggttca cgtcttcgtt acagataggc atgatcaacg
    20221 ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcagat atactgtatt
    20281 tatcgcgacc aataagcagt tgcaggaagc ccattaacgc cgtccagaat ccggcggaca
    20341 cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata taaggcagaa
    20401 gctgcataaa ggaaacccac aaatcctgac caaccatatc cataggatta atcagcgccc
    20461 aaccctgata aggaagaatg gtcttcatat accaggtcgc gacgacagtt tgcgcgagcg
    20521 tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt tgctcgctct
    20581 cctgctcttc tttggtcaaa cgagccagat agcgcggcgt gacatcgcgt ccacgcaggc
    20641 gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc gaggagcgtt
    20701 ttgcttctgg catcgccttc agctgatcgc gcccttcgtc gtctttaatt aactgtccat
    20761 cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct tgcggggaat
    20821 catcctcccg cgcccaggtg cggtgttcgg catccagcgc ctgatgaacg gcgcggatat
    20881 cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca atgtactcag
    20941 ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt ctgatcggca
    21001 ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat ctttcacttt cacacgcatc
    21061 accagacgcc agcctttggt aaccgggtta tagcgcaccg tgctttcaac tatctcacca
    21121 ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt tttcatctct
    21181 gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gctggcgaat caggttcgac
    21241 tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc tggcgcatgc
    21301 agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc tttacccggc
    21361 tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttggtttc atcgttggtt
    21421 ggaatttcca ccagctcaac gctgccttta ccccactccc ctttcggagt cacccatgcg
    21481 cttggacgaa gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc acgaccgcgc
    21541 tgcaacagac cgaagccttg cgggttctcc atcgagaagc tgctgaccgc taaatgtttc
    21601 gggttattca acggacgcca gatccattcg ccattaccgg catggataga cagaccgtta
    21661 gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg cccaaacagg
    21721 aacatactgg ttaacggtgc aacccccagt ttgccgactt tatcgcgcag atagattttc
    21781 gactgcacat ccacaaccgt gtcacgccct ggcataacca cgaatttgta agcacctgtc
    21841 gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg ttttggacgc
    21901 tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc ggtatcaatt
    21961 gccaggccgc gggcagaaag gccataaacc tgacctgcac caatcacgcg gaaatagctg
    22021 gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg gtaaagcact
    22081 ttaaaaccgg caaaaccaag gtcttttacc gtgtctttgt catgctgaac atcgccgaaa
    22141 gtgaaataat ccgggctgta tttgattcgt ttgactgcgg tggcagtcac ttcatttatt
    22201 ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa tggggtcttc
    22261 agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc cgcgtatttc
    22321 atatcgcgga aaacggaggg caagttgctt ttgggcgcct catagccttt cccggctaag
    22381 gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga tgtatacagg
    22441 gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt atgtttcata
    22501 attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt aatggataac
    22561 cagccattcc gacaacagaa tcccggtatc gttcagcacg ctggctcatg gattaagcac
    22621 ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat cgacttcgtg
    22681 tagggtttgc cagcaatttt atttatcaac cgtcttacag acgcttccat tctggttagt
    22741 ggttgcgatg atgctatgca taaaaacctt gaagcctgcg cctggcgcgg tcaggtcggt
    22801 gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa aaatccgaga
    22861 ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctt gactccatcc
    22921 gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat tcgagccata
    22981 catggcatgt gaatagcgcc gaaccgtcat tgtggctgac cctttttaat gacttcatcc
    23041 actcgttccg catgcaggta tttttcgtta tatccggcta cttttcctac atgctttttt
    23101 tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt atcccgatgt
    23161 taacagccat ccccttactg acattgccgc aatttattat gctgcaatac gtcaaaggga
    23221 aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgttg gcctgggaat
    23281 taatatcaca cctgtggttt ttactggtgt tagtggtcat gacgacgctg tgcgtatgga
    23341 tatttaaacg catcagaaat aatttagaaa attctgataa aacgaataaa aaattctcga
    23401 tggtaaaact atcggtgatt tttttgtgcc tcggcatcgg ttatgcggta ataagaagaa
    23461 cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt attgtcatgc
    23521 aaacgctatt ttatttaccg ttctttatcc tcggcgcact ggctttcatt ttccctcatc
    23581 ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct cgctgcagca ttggcgtttg
    23641 tcgcttattt actcaaccag cgctatggca gtggcgatgc ctggatgtac gaaaccgagt
    23701 cagtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc tttggccacc
    23761 gtttgcttaa cttccagtca gcgcgggtga cttactttgt taacgcatcg ctatttatct
    23821 atctggttca ccacccgtta acgctgtttt tcggcgcata cattacaccg cacatcacct
    23881 ccaactggct tggttttctc tgtggcctga tattcgtagt agggattgcg ataattctgt
    23941 atgaaattca tttacgcatc ccgttactga agtttttgtt ttctggtaaa ccggttgtta
    24001 agcgtgagaa cgataaagca ccagcccgtt aagccacatt tacaataacc attccacggg
    24061 caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg gttctttttt
    24121 gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc acctgtccag
    24181 acggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa ttaactgtgc
    24241 cagcgtttcg ctctctatca cgaagcccat ttcagtattg agcaatgttg aacgcggatc
    24301 gaaattgaaa gagccgataa acaccgtttt accatcgatg ctaaaagttt tagcatgcag
    24361 gctagctccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt gttcacgcgt
    24421 cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc agcgcgcgta
    24481 tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga tcgcaatctt
    24541 tacccctttt ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg gtacaaaata
    24601 ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca ggcgctgcgg
    24661 taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac ttaataaacg
    24721 tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatttataa acggactgga
    24781 ttccatttgg cgtaagtaac gatgcgtcat ggcatcgtta tgccaggagg cgggtaactc
    24841 gatgcgatcc gccatttcac cttccgggac atccagcacc tgctgtaagg gtgaaaccga
    24901 tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctaccacgg gtcctattgc
    24961 cataacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg catcaccaat
    25021 atttcgtcct cccaccaggg taaccacgcc atcgacagtg aaacttttat tgtgcatacg
    25081 gcgattgaga cgggaaaagt cggtgatata accaagcgga cgcagcaagc gaaacgagaa
    25141 aggattaaaa agccggactt caatgcgggg atggctgtca agcaagcgta aaatatcgtc
    25201 aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac gctttgctgc
    25261 ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga tgtaatactg
    25321 aacatccagc gtatgctctg ccatttcggc cagacgatac cgggctgcaa acgcatccag
    25381 acttttctcc agaggaaaaa ggccacactg accgggatgt tgcgaacaca gtggcagcac
    25441 cgcgctcgcc agccggggca aatcattcat ctccttgttg agtaaggagt ctttcgtaga
    25501 ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa gcgtgacggg
    25561 taataaattc tgaaacggtt tttactgcga tttcagccgc tgccgcacga gggtaaccat
    25621 aaaccccagt actgattgca ggaaaagcca ctgacgtaaa gctgtttgcc gccaccagtc
    25681 gtaggctatt gagataggca tcctgcaaaa gctggtcttc attttgttca ccaccacgcc
    25741 agactggccc gacggtgtgc actacggctt tagcaggaag atcgcctgca agcgtaataa
    25801 cggcatgccc cgtagggcaa tcgccctgct gttgcctgac ttttaaacaa gcatccagca
    25861 gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccgcctccc attaatgacg
    25921 gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc tgcacaacat
    25981 gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcttaagtgt acagcaggct
    26041 ttttggttaa atcgcatttt tctgctcagc aacgtgagtt gttcagaaac ttagttatat
    26101 tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt tactgaggct
    26161 atttgcgcgt ttttgacatt gccgctcatt aacgctttac tggagtaggt tttgcccgga
    26221 atagaaccat tctgtgggca gttcatttct acgggtgatg actgggtgct gacgtcacat
    26281 gccggttcca caatttggcc ataaaaatgg atcacgcccc cttgcacggt gcttccagca
    26341 atggcaataa aaggaaaaaa caaactgccg agcataagag agtttaaaaa aggtctgaac
    26401 atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc atactttcag
    26461 gttggctgat tcgcagcaga ccattctctc cagattcatc ttatgctcga tatttcaaca
    26521 aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtga ctgtccatca
    26581 gaaacagtaa caactatttt cacccgatcg tccggggaaa tatttaaact caacctcgtc
    26641 aaagcaatgg gttgattagc aggcaatgaa agggtctttt cttgcttcgt ctgactttgc
    26701 cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga ttgagtaaga
    26761 gtgacttcag gaataatggt atacacatcc ccttgctggg tcgtattaaa ggttatctga
    26821 ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa aaaaacaggg
    26881 cttgcgccct gtttctgtaa tacaaatgat gtattagtac tgatgagcgg tcgcgttgtt
    26941 accaaagcca acctgagtca cgttgacgga ggagttagat gcagtctggt caactgcagc
    27001 accgttgcca ccaccgaact gtttaaccgt catttcagaa tttttgccgt tccactgatc
    27061 aagagtagcg ctgttaccga agccacgttg ggtcagatcg attgagctgt catctgagcc
    27121 ctgaccaaca tctgcaccat taccgccgcc atgctgggta atagtcaagt cagagttacg
    27181 ggcatcagtt tgcagagcaa gtgcagagtt accgccaccg tactggtaaa tgttcagctc
    27241 aaaatttggg ccgctattat taccgccacc accgtggtta ccgccgccgc cgtactgagg
    27301 aacaacacct gccagagcgc taccggagaa tacgattgct gcaattgctg ctacttttaa
    27361 aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat taacgttgtg
    27421 tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt tgagtaccat
    27481 actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca gtattaccat
    27541 aagcaccttg cgaaatactg gcatcgttgg cactgcccgc ctgatcaata tatgcaaggt
    27601 tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc gcaacaaccg
    27661 ccaaaagttt tgagcctccc tgccgtaact gagcactatt attagtccca gcttgaccaa
    27721 ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag ttatattctg
    27781 aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt attgttaaca
    27841 tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac atttaagaaa
    27901 ttaaatcatt tcaacttggt tgttaacgca acctgtattt tgttaacgct gcgttacgat
    27961 ggaaagtatg tctgcggaaa tatttttaat aactcacccg cgcgttgtat ttttcttttt
    28021 tagttcatac caaagtatta aaacccacgt aaatacgctg ttatctacgc aaaaaaatat
    28081 tttgttttct tttaaatctc cgttttccgc tatcaaaaag caccagacag tcattcttct
    28141 tgcccgtcgc tgattgctgc gatatgtctt gcgcacaagc cgtgacaaac ctcgtctttt
    28201 tgcagtaaaa aattgtccac ggaggtgtgg agaaaaaaca agaacgtttt acatgacgaa
    28261 aggactacac cgaaatattt tttatatgca ttattagtaa gttatcacca tttgtatgat
    28321 tttttaaaat tgtgcaataa aaaccaaatg tacaactttt ctatcatttc taaacttaat
    28381 aaaaccttaa ggttaacatt ttaatataac gagttacatt tagttacatg tttaacactt
    28441 gatttaagat ttgtaatggc tagattgaaa tcagatgtaa tccattagtt ttatatttta
    28501 cccatttagg gctgatttat tactacacac agcagtgcga catctgtcag tacttctggt
    28561 gcttctattt tataggcagc tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca
    28621 tgtttaatga agtccatagt attcatggtc atacattatt gttgatcact aaaccttctt
    28681 tgcaggcgac agctctcttg cagcacctta aacaatcgct ggcaattaca ggaaaattac
    28741 ataatattca acgttctctg gacgatatct cttcaggctc tattattctt ctggatatga
    28801 tggaagcgga taaaaagctt atccattatt ggcaggatac tttgagcagg aaaaacaaca
    28861 atatcaaaat attgttgcta aatacgcctg aagattaccc gtaccgcgac attgaaaa
//
LOCUS       DACAPB010000040        28689 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.040, whole genome shotgun sequence.
ACCESSION   DACAPB010000040 DACAPB010000000
VERSION     DACAPB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.040"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(159..246)
                     /locus_tag="IT904_001589"
     tRNA            complement(159..246)
                     /locus_tag="IT904_001589"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Ser,seq:gga)
     gene            complement(500..718)
                     /gene="infA"
                     /locus_tag="IT904_001590"
     CDS             complement(500..718)
                     /gene="infA"
                     /locus_tag="IT904_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP3191386.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(1003..1707)
                     /gene="aat"
                     /locus_tag="IT904_001591"
     CDS             complement(1003..1707)
                     /gene="aat"
                     /locus_tag="IT904_001591"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAP3191387.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1749..3470)
                     /gene="cydC"
                     /locus_tag="IT904_001592"
     CDS             complement(1749..3470)
                     /gene="cydC"
                     /locus_tag="IT904_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAP3191388.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTHAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3471..5237)
                     /gene="cydD"
                     /locus_tag="IT904_001593"
     CDS             complement(3471..5237)
                     /gene="cydD"
                     /locus_tag="IT904_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAP3191389.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5360..6325)
                     /gene="trxB"
                     /locus_tag="IT904_001594"
     CDS             complement(5360..6325)
                     /gene="trxB"
                     /locus_tag="IT904_001594"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP3191390.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTKRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6870..7364
                     /gene="lrp"
                     /locus_tag="IT904_001595"
     CDS             6870..7364
                     /gene="lrp"
                     /locus_tag="IT904_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAP3191391.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7499..11488
                     /gene="ftsK"
                     /locus_tag="IT904_001596"
     CDS             7499..11488
                     /gene="ftsK"
                     /locus_tag="IT904_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP3191392.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDAEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            11643..12254
                     /gene="lolA"
                     /locus_tag="IT904_001597"
     CDS             11643..12254
                     /gene="lolA"
                     /locus_tag="IT904_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAP3191393.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12265..13608
                     /locus_tag="IT904_001598"
     CDS             12265..13608
                     /locus_tag="IT904_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP3191394.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13699..14991
                     /gene="serS"
                     /locus_tag="IT904_001599"
     CDS             13699..14991
                     /gene="serS"
                     /locus_tag="IT904_001599"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP3191395.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15230..17674
                     /gene="dmsA"
                     /locus_tag="IT904_001600"
     CDS             15230..17674
                     /gene="dmsA"
                     /locus_tag="IT904_001600"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAP3191396.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17685..18302
                     /gene="dmsB"
                     /locus_tag="IT904_001601"
     CDS             17685..18302
                     /gene="dmsB"
                     /locus_tag="IT904_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP3191397.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18304..19167
                     /locus_tag="IT904_001602"
     CDS             18304..19167
                     /locus_tag="IT904_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP3191398.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19202..19828)
                     /locus_tag="IT904_001603"
     CDS             complement(19202..19828)
                     /locus_tag="IT904_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP3191399.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRLSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20142..21290
                     /locus_tag="IT904_001604"
     CDS             20142..21290
                     /locus_tag="IT904_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191400.1"
                     /translation="MSTYTQPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21500..22930
                     /locus_tag="IT904_001605"
     CDS             21500..22930
                     /locus_tag="IT904_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3191401.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22931..23839)
                     /locus_tag="IT904_001606"
     CDS             complement(22931..23839)
                     /locus_tag="IT904_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191402.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            23939..24528
                     /locus_tag="IT904_001607"
                     /pseudo
     CDS             23939..24528
                     /locus_tag="IT904_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
     gene            complement(24610..25407)
                     /gene="pflA"
                     /locus_tag="IT904_001608"
     CDS             complement(24610..25407)
                     /gene="pflA"
                     /locus_tag="IT904_001608"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAP3191403.1"
                     /translation="MSNLTNCNTTENTAAAGATKSVIGRIHSFESCGTVDGPGIRFIT
                     FFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAIL
                     QAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQN
                     LVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIEL
                     LPYHELGKHKWVAMGEEYKLDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(25439..26434)
                     /locus_tag="IT904_001609"
     CDS             complement(25439..26434)
                     /locus_tag="IT904_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3191404.1"
                     /translation="MSIKKLDDGRYEVDVRPQGADGKRIRRKFKTKGEAQAFERHVLV
                     NYHNKEWLEKPADRRTLTELLGRWWIYHGKSHERGDIERGRLTTIIAKFAEMGVSRAD
                     QLTKKTITDYRVVMMNDGLKPASVNRHLAIMSGMFTKLIDAGEYHSHNPFREVKRLRE
                     AVTEMAFLSSEEITRLLSMLDGDELNATLVCLSTGGRWSEVSNLKAEHIINQMVTFMK
                     TKNGKRRTIPVSQDLIKRIKTKNSGRLFNASYYKVRNALREVKPDLPDGQAVHVLRHT
                     FATHFIMNGGNIITLQRILGHSNIQQTMTYAHFAPDFLQDAVTLNPVSGMSIMRP"
     gene            complement(26528..26839)
                     /locus_tag="IT904_001610"
     CDS             complement(26528..26839)
                     /locus_tag="IT904_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3191405.1"
                     /translation="MSNTQITSQAEKLALIRESERMTRKQVAELTGINYNTYAGYEQG
                     KVKMSFDAGMKFFKPERFRKYRDWFMFDETDPAGGQIAPALAHIGQDSTTLHHSDQKT
                     G"
     gene            26944..27300
                     /locus_tag="IT904_001611"
     CDS             26944..27300
                     /locus_tag="IT904_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191406.1"
                     /translation="MSIGSEMNNDVGEKVSDLTKSKKCDIKLAAAPSDLLSKEGFALY
                     IGKTPRAVAEMAKAGKLPAFYMTDPLKPGGHAELWINRREWDKYAAQLVDEAPTEWHD
                     WKNRICYSKSRHGRAA"
     gene            27478..27978
                     /locus_tag="IT904_001612"
     CDS             27478..27978
                     /locus_tag="IT904_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HAP3191407.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            28042..28266
                     /locus_tag="IT904_001613"
     CDS             28042..28266
                     /locus_tag="IT904_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HAP3191408.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            28266..28568
                     /locus_tag="IT904_001614"
     CDS             28266..28568
                     /locus_tag="IT904_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191409.1"
                     /translation="MSCSRSVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKNLNDKRNW"
     gene            28568..>28689
                     /locus_tag="IT904_001615"
     CDS             28568..>28689
                     /locus_tag="IT904_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HAP3191410.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAP"
BASE COUNT         7169 a         6990 c         7409 g         7121 t
ORIGIN      
        1 gaacaatctt gcaaaggctg tgaaagttgg cgttcactgc cgtacaggca gcttagaaat
       61 tcacaggtaa catactccac ccaccatgtt cactgccgta cagacagata aaatgcgaaa
      121 aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg ggggattcga
      181 acccccgata cgttgccgta tacacacttt ccaggcgtgc tccttcagcc actcggacac
      241 ctcaccaaat tgttttgctg ccaaacctca tgggtggcaa cggggcgcta ctatagggag
      301 ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca tactgtaaac
      361 aacttgtgca gtatatctac atcgagacaa gttatggact tatacttcca aagtacttca
      421 tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct ttctcttcgc
      481 ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg ctcaggtcgt
      541 acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag tttttgcgca
      601 ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc taactctacg cggaacatgg
      661 tattaggcaa cgtttcaaga acggtacctt gcatttcaat attgtcttct ttggccatct
      721 aatcctctgg ggtatcacta ccgtaatttg aaccggcaag ataatgccga agttctgtaa
      781 ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt cggcacatta ctccgaaaac
      841 acacggctaa gccgcaccaa aagcgcaacg tataagggag cggtgagata aacgatgggc
      901 gttacctgac gcgaaaaatt ccttatcggc agcggggtaa tgagcgtaac caactctgcg
      961 accgcaatta taacactctg gggagaaatg tgccgaaaac attcattctt gtggtgaaaa
     1021 caagcaccgt ggtacccaga aattattcgg caatcgtccg aggcgcattt gattgagata
     1081 attaaggtaa tcccggcggg gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag
     1141 gacctggcag tcgataagct taccgccatg accgataaat tcctcacaga ataccagaag
     1201 cgccgtttta gacgcatttt ccatccggct gaacatggac tcgccacaaa atagtgttcc
     1261 ctgggccacg ccgtacatac cgccgacaag ctcatcttca cgccagactt caatggagtg
     1321 ggcatgaccg agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg tgatccaggt
     1381 tccttcttcg cgatcgctgg cacagccttc aatgacctga ccaaaagcgt aattcatcgt
     1441 gacacgatag ggcgagcgtt tatgaaatcg cttcatacta cggctgatat gcagtgattc
     1501 tggccatagc accgcgcggg gatcgggcga ccaccagagg atggggtcgc ctggagaaaa
     1561 ccacggaaaa ataccacgct ggtaagccat taacaggcgc gcagggctaa gatcgccccc
     1621 aagtgccagc aggccgttag gctcacgtaa tgcgccttcc ggggaaggga aggctattga
     1681 atggcgagaa agctgaacca ggcgcatgac cgcaaaactc cacgcaagtc ggatcgttca
     1741 ataatagctt acaaaccctg cttgaactgg taataacgcc cctgtctggc aagcagttct
     1801 gcgtgagtac cttgctcaat aatttgcccg ttgtccatca ctattatttg ttggaaacga
     1861 gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt tctcacgcat catttctgca
     1921 agcaattcaa ggatctggct ttcggttgtg gcatctaagc cttcggtagg ttcatccagc
     1981 aacaccagtg gcgcatcatg taacagcgca cgggcgatag ccagacggcg cagttcacca
     2041 ccggagagct ggcgtccgcc ttcacctaac caactgttga gacctgcatc ctcgagcagc
     2101 ttctccagac caacgcgacg caagatctcc gacagagcct catcactact gccaggcgag
     2161 gcgagtaaca gattatcacg cagcgtggcg ctaaacagat gcactcgctg aggaacaacg
     2221 ctgatggtct gtcgtagagc cgcttcattc aggctggcta tggggctatc gttaagcaaa
     2281 atctcgccct gttgcgggtc ccaagcgtgg gtcagcagtt gtaacagtgt tgatttgccg
     2341 catccggttc gcccgagaat cgcaatatgt tccccggcgt ttacctgaag agaaatccct
     2401 ttaagtgcct gttgggattg ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa
     2461 acgcgatcgg caacacgagt ttgggtatca gggaaggtga cttccggttt ttgatccgtt
     2521 aaatcagtga tccgaacggc agaggcaatg acttgcccca gatgctgaaa tgcacccgtt
     2581 actggtgcca gtgcttcaaa cgcggctaac gcgcagaaga caaacagggc aattaacgcg
     2641 ccgggttgag cattgccgcc aacgccgcca gacgccatcc acagcatcag gatcaccgct
     2701 aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt
     2761 tgcgcttcca gccattgaat ttctgtattc tctagttgcg tgcgataacg atcgctggca
     2821 ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg tcagttgttg gcgatactgt
     2881 ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg cacgataaaa cagcggtggc
     2941 atcaggaaaa gcgtcagtaa cataatgccg cccagcgtaa aggcgagggt gaaatcaagg
     3001 aaacttaacc cgattgtcac caccataatc accacaaaag cgcccaccag cggcgagata
     3061 acgcgcaggt aaagatgatc gagcgtatca acatccgcca ccacgcgatt gagcaattcg
     3121 ccctgacgat agcgcgccag tccggcaggg gagaggggca gcaatttgct gaaggtgtaa
     3181 atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaatag
     3241 cgcccggcag tacgggtgat tgctgcgcca cgcacgcccg cagcgggtag catatagttg
     3301 aagctgtaca gtccggcaac ccccgcaacc gctgaggccg agaggaacca gccggaaagt
     3361 gtcaacagac cgatactggc gagcagcgtc acaattgcca gcacaatacc aagacttaac
     3421 atccatttat gacgtttata cagtgccaga tagggtagca aagcgcgcat ttaaatctcc
     3481 tcctgacgat gggccagtaa tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa
     3541 cgtccttgct caataatccg gccatcctgc ataacccaaa tgacatccca gtcagcaaga
     3601 tcttctaact ggtgggtgac cattaacgtt gtctggcgca gagaggcggc attcagcgcc
     3661 tccattacgc gctgttcact gtgagcatca aggctggcag cgggttcatc caacagtaat
     3721 agcgaacagg gatttagtaa cgcacgggcc accgccacgc gctgcgcctg ccccacggaa
     3781 aggcgggcag cctggtcgcc aacaggcgta tcaacgcctt gtggcaggag cggtagaaac
     3841 tcgctgaccc aggcgttatc cagcgctgct tgtaattctt gttcgctggc atcaggtcgc
     3901 gccagtagta cgttatcccg taatgttgct gccggtaatt gtgggttttg cccaacccag
     3961 gagagatgtt tacgccatga ttctggtgat aaatcgcgta attctatccc gttgattcgt
     4021 aatgatccct gatatgagag aaaaccagaa agcgcgttca gcagtgagct tttacctgaa
     4081 ccgctgcgac caaccaacac tgcacgttgg cctgctggca aagtaaagtt cagcggtccg
     4141 gccagcgttt taccttccgg cgacgtgata aacagatcct cggcctcaat ggtcagcgga
     4201 tcggtcaatg ctaattccgc ctcaccgcgc tgcggatggg cgagcggggt ttccataaac
     4261 gttttcagac tgtcagctgc accaacagcc tgggctttag catgataaaa cgtaccgaga
     4321 tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc cgcagccagc
     4381 gtcacgccag tatcgtagtg accaaaatcc agctcgccga gataggaaaa accaaagtag
     4441 accgccacca gagcaattga cagcgaggta aaaaattcga gaatgccgga ggataaaaac
     4501 gccagtcgta gcacttccat tgtccgttgg cggaaatctt ccgaagcaga acgaatactt
     4561 tcaatttcag cttcaccacg accaaaaata cgcaatgttt ccatgccgcg caggcgatcg
     4621 aggaaatgcc cacttaagcg agcaagagcg agaaagttac gtcggttagc atcggcagcc
     4681 cccattccaa ccagcgccat aaacaacgga attagcggtg ctgtgcccag cagaatgagc
     4741 gccgcagccc agttagaggg gaagatcgcc accacaatca gcaacggcac cgacactgcc
     4801 agcgccattt gcggcagata gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt
     4861 accagcgtcg cccagctccc cgcaggttta ccctgaatcc acgctggccc tgcttgttgc
     4921 agacggtcga gaacctgacg gcggatggca aagcggatat gctgcccggc gtgataaccc
     4981 acccgttcgc gtaaccagac cacccatgcg cgcagtacaa aggtcagaac cagtaacgta
     5041 aagggaagca gcagggcttc acggggaata ttctccataa tcatatgttg cagaattcgc
     5101 gccatgaacc aggcctgggc aatgatcaat atgccgctca caaagcccag cagacgagaa
     5161 atattcagcc aacgttggga gatgacgctt tgctgtttta accagcgggt taactctttt
     5221 tgacgagatt tattcattgc acgcttagca ggtgagttat cagaattatt tgcagagcaa
     5281 tgttacaacg gggaaaaaat aaaggcgacc catagtcgca tggtgtcgcc ttctttactt
     5341 ttgttactga tttgtaaaat tattttgcgt cagctaaacc atcgaggtag cgttccgcat
     5401 caagtgctgc catgcagcct gtaccggccg aagtaatggc ctggcgataa atgtgatcca
     5461 tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg ggtggcatta ccatgaatac
     5521 ccgactgtac tttgatgtag ccgttttcca gttccagctg cccttcgaaa atcgcagtat
     5581 tcgggctgtg accgatagca acaaacagac cggcaacgtc gagtgactcg atgttatcgc
     5641 tgttttgcgt atcgcgcaga cgaacgccag tgacacccat ttgatcgccg gtcacttctt
     5701 ccagcgtacg tttggtgtgc agaatgatgt tgccgttctc cactttatcc atcaggcgct
     5761 taatgaggat tttttccgcg cggaaaccgt cacggcggtg aatcagatgc acttccgaag
     5821 cgatgttaga cagatacagc gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa
     5881 ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt
     5941 taaaggcttc ttcagagggc aggccgagat agcgtgcaga agctccggtg gcaataatca
     6001 gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg gaacggacgg ttttgcagat
     6061 ccaccttgtt gatatgatca aaaatgatct cagtttcaaa cttggtggca tgttcgtgca
     6121 tgcgctccat taataacgga ccggtcagat cgtttggatc gccaggccag ttttccactt
     6181 ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt aatcagcaca ggttgcaggt
     6241 tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc aggataagca
     6301 gtttactgtg tttggtcgtg cccatgagat ccccatagtt gttggcagac aatgggcagg
     6361 attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca
     6421 atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg ctacttaaat
     6481 ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga atatctggca
     6541 tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc gaaaacattc
     6601 gaggaagaaa aaaaacagta ttcttatatg cgcataacca tgcatgtaaa taccatgttt
     6661 accgtgctag tgaaatctac gtatggcgtg gacagacgcc attcgtgatg tcgatagctg
     6721 ccacaaggca acggtcttct caccgtagac ccaggcattg cgcgccgtga atcttcatga
     6781 tttcggtcta tcgtgactgg tagcgactct gaacagtgat gtttcagggt cagacaggag
     6841 tagggaagga atacagagag acaataataa tggtagatag caagaagcgc cctggcaaag
     6901 atctcgaccg tatcgatcgt aacattctta atgagttgca aaaggatggg cgtatttcta
     6961 acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc
     7021 ggctggaaag acaagggttt attcagggct atacggcgct gcttaacccc cattatctgg
     7081 atgcatcact tctggtattc gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg
     7141 aacaattcaa taccgctgta caaaaacttg aagaaattca ggagtgtcat ttagtatccg
     7201 gtgatttcga ctacctgttg aaaacacgcg tgccggatat gtcagcctac cgtaagttgc
     7261 tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac acggacatac gttgttatgg
     7321 aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa
     7381 aatcggcgta ttttgattac actcctgtta atccatacag caacagtact ggggtaacct
     7441 ggtactgttg tccgttttag catcgggcag gaaaagcctg taacctggag agcctttctt
     7501 gagccaggaa tacactgaag acaaagaagt cacattgaca aagttaagta gcggccgccg
     7561 ccttctggag gcgttgctga tccttattgt cctgtttgcc gtctggttga tggctgcctt
     7621 actaagcttt aacccttcgg accccagctg gtcgcaaacg gcctggcatg aacctatcca
     7681 taatttaggt gggatgcccg gtgcgtggtt ggcagatacg ctgttcttta tttttggcgt
     7741 gatggcttac accattcccg tcattattgt cggcggttgt tggtttgcct ggcgtcatca
     7801 gtccagcgac gaatacattg attattttgc cgtttcgcta cgcatcattg gcgttttggc
     7861 gctcatcctt acctcctgtg gtctggcggc aatcaacgct gacgatatct ggtattttgc
     7921 ctccggtggc gtcattggca gcttactaag cactacgcta caaccactgc tacacagtag
     7981 cgggggaact attgcgctgc tctgcgtttg ggcagcgggc ctgacgttgt tcaccggttg
     8041 gtcatgggtg accattgctg aaaaactcgg cggctggatt ttaaacattc tcaccttcgc
     8101 cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg aagacgacgc
     8161 agagtatgaa gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc gtattcttcg
     8221 cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc attaatccga tggggcggca
     8281 aacagacgct gcgttgttct ccggtaagcg gatggatgat gacgaagaga ttacctacac
     8341 tgcacgcggt gtggctgccg acccggacga cgtcctattt tcgggcaatc gtgcaacgca
     8401 gccagaatat gacgaatacg atccattatt aaacggtgcg ccaattaccg aacctgtcgc
     8461 tgtagcagct gctgctacca cggcgacaca aagctgggct gcgccggttg aacctgtgac
     8521 tcagacgccg cctgttgcct ctgttgatgt tccacctgcg caacctacag tagcctggca
     8581 gcctgtaccg ggtccacaaa cgggagagcc ggttattgct cctgcaccgg aaggttaccc
     8641 acagcagtca caatatgcgc agcctgcagt gcaatataat gagccgctgc aacaaccagt
     8701 acagccgcag cagccgtatt atgcacctgc agctgaacaa cctgcgcaac agccgtatta
     8761 tgcccctgcg ccagaacaac cggtggcagg taacgcctgg caagccgaag agcagcaatc
     8821 cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc cagccgctca
     8881 ggagccgttg taccaacagc cgcaacccgt tgaacagcag cctgttgtgg agcctgaacc
     8941 cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac tactttgaag aagttgaaga
     9001 gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc cagaaccggt
     9061 taaagaacca gaaccgatca aatcttcgct gaaagcacct tctgttgcag cagtacctcc
     9121 agtagaagcc gctgccgctg tttccccgct ggcatctggc gtgaaaaaag cgacactggc
     9181 gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata gcggtggacc
     9241 gcgtcctcag gtcaaagagg ggattggtcc gcagttgcca cgaccgaaac gtatccgcgt
     9301 gccaactcgt cgtgaactgg cgtcttacgg tattaagctg ccctcacagc gtgcggcgga
     9361 agaaaaagcc cgtgaagccc agcgcaatca gtacgattct ggcgatcagt acaacgatga
     9421 tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga cacagcagca
     9481 acgctatggc gaacagtatc aacatgatgt gcccgtaaac gcagaagatg cagatgctgc
     9541 ggcagaggct gaactggctc gtcagtttgc gcaaactcaa caacaacgtt attccggcga
     9601 acaaccggct ggggcgaatc cgttctcgct ggatgatttt gaattttcgc caatgaaagc
     9661 gttgctggat gatggtccac acgaaccgtt gtttacgcca attgttgaac ctgtacagca
     9721 gccgcaacaa ccggttgcac cgcagcagca atatcagcag ccgcaacaac cagttccgcc
     9781 gcagccgcag tatcagcagc cacaacagcc ggttgcgccg cagccacaat atcagcagcc
     9841 gcaacaaccg gttgcgccac agcagcaata tcagcagccg caacaaccgg ttgcgccgca
     9901 gcagcagtat cagcagccac aacagccagt tgcgccacaa ccgcaggata ccctgcttca
     9961 tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga cgccgctgcc
    10021 ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata cctttgcgct
    10081 tgaacaaatg gctcgcctgg tggaagcgcg tctggctgat ttccgtatta aagccgatgt
    10141 cgtcaattac tctccggggc cggttatcac tcgctttgaa ttgaacctgg caccgggcgt
    10201 aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt cgacggtggc
    10261 ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt taccgaataa
    10321 aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc gcgataatcc
    10381 gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg ttgccgatct
    10441 ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat ctgtcggtgt
    10501 gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc gtttcatcat
    10561 gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc tgttaacgga
    10621 agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga
    10681 gcgtcggtat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa
    10741 aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga agccgggtga
    10801 cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtgg tgttggttga
    10861 cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga tagcacgtct
    10921 ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc gtccatcggt
    10981 tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc
    11041 cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac tgctgggtat
    11101 gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc atggtgcttt
    11161 tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg gtcgcccaca
    11221 gtatgttgat ggcatcacct ccgacagcga aagcgaaggt ggtgcgggtg gtttcgatgg
    11281 cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa
    11341 agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat
    11401 tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg gtaatcgtga
    11461 agtgctggcc ccaccgccgt ttgactaatt aatgcattgc cggataaggc gcggtagcgt
    11521 cgcatccggc actctatcaa ctgaaaattc agtattttct tctttcctca agctgattat
    11581 tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt tgaggaataa
    11641 tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca agcagcgttt
    11701 gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc ttccacgcca
    11761 gcttcacaca aaaagtgact gacggtagcg gcgcggcggt gcaggaaggt cagggcgatc
    11821 tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat gaaagcattc
    11881 tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa gctacggcaa
    11941 cctggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc aaccagtcca
    12001 gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg acgccgaaag
    12061 ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc acaatccatc
    12121 agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcaactgaaa tcccagcaaa
    12181 atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc acggtagatg
    12241 atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc ggataatact
    12301 tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat cggccagcaa
    12361 catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca tttacattct
    12421 atgatcctct gggggccgcc gggtaccggc aaaacaactc tcgctgaagt gattgcccgc
    12481 tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa agagattcgc
    12541 gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat tctttttgtt
    12601 gacgaagttc accgtttcaa caaaagccag caggatgcat ttctgccaca tattgaagac
    12661 ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct taattcggca
    12721 ctgctttccc gtgcccgtgt ctatctgttg aaatccctga gtacagagga tattgagcaa
    12781 gtactaactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga tattgttctg
    12841 ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg ccgggcgtta
    12901 aatacgctgg aaatgatggc ggatatggcc gaagtcgatg atagcggtaa gcgggtcctg
    12961 aagcctgaat tactgaccga aatcgccggt gaacgtagcg cccgctttga taacaaaggc
    13021 gatcgctttt acgatctgat ttccgcactg cataagtcgg tacgtggtag cgcacccgat
    13081 gcggcgctgt actggtatgc gcgaattatt accgctggtg gcgatccgtt atatgtcgcg
    13141 cgtcgctgtt tggcgattgc gtctgaagac gtcggtaatg ccgatccacg ggcgatgcag
    13201 gtggcaattg cggcctggga ttgctttact cgcgttggcc cggcggaagg tgaacgcgcc
    13261 attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt ctacactgcg
    13321 tttaaagccg cgctggccga tgctcgcgaa cgcccggatt atgacgtgcc ggttcatttg
    13381 cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata tcgttacgct
    13441 catgatgaag caaacgctta tgctgccggt gaggtttact tcccgccgga aatagcacaa
    13501 acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga aaagctcgcc
    13561 tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg ttatcgttgc
    13621 ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc catttaattc
    13681 gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc agacgcagtc
    13741 gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg cgctcttgaa
    13801 gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg taactcccga
    13861 tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt acgtctggaa
    13921 gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc tttacaggct
    13981 gaaattcgcg atatcgcgct gaccatccct aacctgcctg cagatgaagt gccggtaggt
    14041 aaagacgaaa atgacaacgt tgaagtcagc cgctggggta ccccgcgtga gtttgacttt
    14101 gaagttcgtg accacgtgac gctgggtgaa atgcactctg gcctcgactt tgcagctgca
    14161 gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg catgcaccgc
    14221 gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag tgagaactat
    14281 gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcaactgcc gaaatttgct
    14341 ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa ctatgcgctg
    14401 atcccaacgg cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat cgatgaagat
    14461 gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc cggttcatat
    14521 ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga aatggtgcag
    14581 atcgtgcgcc cagaagactc aatggcggcg ctggaagaga tgactggtca tgcagaaaaa
    14641 gtcctgcagt tgctgggcct gccgtaccgt aaaatcatcc tttgcactgg cgacatgggc
    14701 tttggcgctt gcaaaactta cgacctggaa gtatggatcc cggcacagaa cacctaccgt
    14761 gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca ggcacgttgc
    14821 cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc tggtctggct
    14881 gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg tcgtattgaa
    14941 gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta atacccaatt
    15001 tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat accgaacaat
    15061 aattactcct cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat
    15121 tttcaaatta ttcgatgtat acaagcctat atagcgaact gctatagaaa taattacaca
    15181 atacggtttg ttactggaat caatcgtgag caagcttgag tgagccatta tgaaaacgaa
    15241 aatccctgat gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa aaacgacagc
    15301 gatcggcggc ctggcaatgg ccagcagcgc attaacatta ccttttagtc ggattgcgca
    15361 cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga gcgcctgtac
    15421 agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg aaatcaaata
    15481 tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc ctgcaccagg ttcgcgcctg
    15541 cctgcgtggg cgttccatgc gtcgccgtgt ctacaatccg gaccgcctga aatatccgat
    15601 gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta
    15661 cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg agtctatcta
    15721 tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc cgccgggaaa
    15781 taccctggtc gcgcggctga tgaactgctg cggcggctat ctgaaccatt acggcgacta
    15841 ctcctccgcg caaattgcgg aaggtttgaa ctatacctac ggcggctggg cagatggcaa
    15901 cagcccgtcg gatatcgaaa acagtaagct ggtagtgctg tttggtaata accctggcga
    15961 aacgcgaatg agtggcggtg gggtgactta ctatcttgaa caggcacgcc agaaatctaa
    16021 tgcccgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc gcgaagatga
    16081 gtggatccct attcgtccgg gaacagatgc cgcactggtt aacggtctgg cgtacgtcat
    16141 gatcactgaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctatgatga
    16201 gaaaaccctg ccagccagtg cgccgaaaaa tggccactat aaagcttata ttctgggtga
    16261 agggccagat ggcgtggcta aaacgccgga atgggcctcg caaatcactg gtgttccggc
    16321 agacaaaatc atcaaattgg ctcgtgaaat cggtagtacc aaaccggcgt ttatcagcca
    16381 gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct
    16441 ggcgattctg accggtaacg ttggtattaa cggaggcaac agcggcgcgc gtgaaggttc
    16501 atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccgatccaga ccagcatttc
    16561 gatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc tgcgtgatgg
    16621 tgtacgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa
    16681 ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatccttc aggatgataa
    16741 gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga aatatgctga
    16801 catcctgctg cctgactgca ccgcttccga acagatggac tttgcgctgg atgcatcctg
    16861 cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa
    16921 gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac
    16981 tgaaggccgt acccaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat
    17041 tcctgaactg ccaacgtttg aagagttccg caagcagggg atctttaaaa agcgcgaccc
    17101 acaagggcat cacgttgctt ataaagcctt ccgtgaagat ccgcaggcaa acccactgac
    17161 tacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg ccgctacctg
    17221 ggaattgcct gaaggcgatg tgatcgatcc actgccgatc tacacgccgg gctttgaaag
    17281 ttatcaggat ccgctgaaca aacagtatcc gctgcagctt acaggtttcc actataaatc
    17341 tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa gcggcttgcc gtcaggaaat
    17401 gtggatcaac ccgcttgatg cccaaaaacg cggtatccac aacggcgata aagtcaggat
    17461 ctttaacgat cgtggtgagg ttcatattga ggcgaaagtg acgccacgaa tgatgccggg
    17521 tgtggtcgca ctgggtgaag gtgcctggta tgacccggat gcaaaacgtg tcgataaggg
    17581 tggttgtatt aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc
    17641 acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg
    17701 gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcctgtaaag
    17761 actacaaaga tttgacgcca gaagtcagct tccgccgcat ttatgaatat gctggcggcg
    17821 actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt
    17881 catgtaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt gcgatgcata
    17941 aacgtgaaga tggttttgtt gtggtcgatg aagatgtgtg cattggctgc cgctactgcc
    18001 atatggcttg cccgtatggc gcaccgcaat ataacgaaac gaaaggccat atgaccaaat
    18061 gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc gatctgtgtt gaatcttgtc
    18121 cgctgcgggc gctggatttc ggccctatcg acgagctgcg taaaaaacat ggcgatctgg
    18181 cggccgttgc gccgttgccg cgagctcact ttaccaaacc gaatattgtg atcaaaccca
    18241 atgccaatag ccgcccgacc ggggatacca ctggctatct ggcaaacccg aaggaggtgt
    18301 gagatgggaa gtggatggca tgaatggccg ctgatgatct tcacggtctt cgggcaatgt
    18361 gtagcaggtg gttttatcgt tctggctttg gcgctgctca aaggcgacct gcgagcagaa
    18421 gcccagcagc gtgttatcgc ctgcatgttt ggtttatggg tgctgatggg cattggcttt
    18481 atcgcctcta tgctccatct tggttcacca atgcgcgctt ttaactcgct caaccgggta
    18541 ggggcttcag cactcagtaa cgaaatcgcc agcggttcga tcttttttgc cgtaggcggc
    18601 atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac gctgtggctg
    18661 atagtgacga tggttcttgg cgtcatcttt gtctggatga tggtgcgtgt gtataacagc
    18721 attgataccg ttccgacctg gtacagcatc tggacgccga tgggcttctt cctgacgatg
    18781 tttatgggcg gcccgttact gggttacctg ttgttgagtc tggctggcgt cgatggctgg
    18841 gcgatgcgtc tgctgccagc aatttctgta ctggcactgg tagtgagtgg cgtggtgtca
    18901 gtgatgcagg gcgcagagct ggcgactatt catagttctg tgcagcaggc cgcagcgctg
    18961 gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt tgccctgtgc
    19021 ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact ttctgtatca
    19081 ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg tttgcatatg
    19141 accgtgggga tggccgtcgc aagctaatca taacaaccgg ggtttcggcc ccggttctct
    19201 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatgt
    19261 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcgcga tgcagctcgc
    19321 acgccacgcc gaaccatgtc atcaattgcg caccagcttg cgacaagcga tcccatgccg
    19381 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacgacaaag acgtcaaacc
    19441 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    19501 taattaactg ttttttacct gtcgctttga cagcttttac aaaatcttcg ttatcccagg
    19561 cgttaatatt tcccgggcga gcaatgtaag gtgtatcggg gaattgtgct ttcagttctg
    19621 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    19681 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    19741 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacggca gcatcatttt
    19801 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgtc
    19861 tgaaatcgat cagacgcgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    19921 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttagt tcaataaaaa
    19981 caatcagatg agactaatca tctcttcagc attagcgtta tgaatgagta atataaatcg
    20041 ataataccgc cgctgggata tcgcgtattt tcacccattg acaatgtttt tggcggtggc
    20101 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccagc
    20161 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgattgcg gtgttaaata
    20221 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
    20281 gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaagc
    20341 gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
    20401 gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc gcgggcgtcg
    20461 gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta
    20521 accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc
    20581 agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg gttacaggtt
    20641 tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag caggctgaaa
    20701 accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga
    20761 atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg ccgctgtacc
    20821 tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg
    20881 cgggtatcct tggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg
    20941 tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt agccaggcgg
    21001 cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
    21061 tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac
    21121 tgttgagcta tactgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga
    21181 atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga
    21241 tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa
    21301 gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt
    21361 tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt ttgcatttcg
    21421 ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg cccctttaat
    21481 tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagttgc gatggtacaa
    21541 cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta ataactatgc
    21601 caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcactct atttcacacc
    21661 ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag
    21721 tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt ggacctactg
    21781 ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg cgctcggttg
    21841 ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt tacaggggtt
    21901 aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa
    21961 aatcgtcggt tctgtggcag ggattgctat gtttgttatg tcgctcctgt atgtggcgat
    22021 ggcggtaacc gcgcctgcaa ttactgaagt gcatattgcg accacaaaca ttacctggga
    22081 aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc
    22141 ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga
    22201 atttccaaaa gggatgttat gcctggcggt gatggttgcg gtttgtgcca ttctgggctc
    22261 gctggcgatg gggatgatgt ttgattcgcg taatatcccg gatgacttaa tgaccaacgg
    22321 tcagtattac gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt
    22381 gatttacgcc attgcgaata ccctgggaca agtagcggcg ctggtattct cgattgatgc
    22441 cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca gtttatgtcg
    22501 taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtac tggtggcgat
    22561 tctgattatg ctgccgactc tcggcattgg tgatatgaac aatctctata aatggctgtt
    22621 gaaccttaat tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc
    22681 agtcgttcgc ttggcgcaga aatataaacc agaatatgtc tttattcgta ataagcctct
    22741 ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgtt tgacggggat
    22801 cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc
    22861 aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc gtaaagcgaa
    22921 tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
    22981 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttggataa tagatctgga
    23041 agcctggacg ctctgtgctc cagtcttcca gtacacattc taaacgacca gtatcaaggt
    23101 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcctcca
    23161 gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc agctcctttg
    23221 caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat tgatgattaa
    23281 gcagatcatg aggatggcgg ggtttgccat atcttgcaaa gtactctggc gttgctgcta
    23341 cacataattt cactggcggg ccgatcgcca cgctgatcat atctttttcg acgatgcaac
    23401 taagtcgaac gccagcgtca aagccctgtt ggacgatatc gacaagagag tcgtcagttg
    23461 tgagctcgac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa gacattaaaa
    23521 atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt gtcagacgaa
    23581 aatcattcat ctcatctaac atgatctgaa tttcgtcgaa agctgggcgc aatcgttcat
    23641 agagattaga acccgcttcc gttaacgata cgcttcgggt tgtccgattg aataagcgaa
    23701 ttttaagacg ttgttccagt gttttaatac tatggctaat ggcggacgag gataagccta
    23761 actcatcgcc cgctgcacga aagctttgat tacgggccac ggcaaagaaa gtggcaaagt
    23821 cagacatatt catccgcatt agtgaaaatc cttcattaac tcatcctgaa tcatcacgtt
    23881 tacaggatta tgccgcctgc gtaaagtgcg ctccagaact taacgtggag gtaaaattat
    23941 gcagtctgaa cgtatttatt tggtatgggc ccatcctcgt catgattcat tgaccgcaca
    24001 tattgctgat gcgatccatc agcgggcaat ggagcggaaa atacaggtga cggaactcga
    24061 tttatatcgg cgtaatttca acccagtgat gacgccggaa gatgaaccag actggaagaa
    24121 tatggataaa cgttattctc cagaggttca tcagctttat tcagagctgc ttgaacatga
    24181 cacgttagtg gtggtttttc ctctctggtg gtacagcttc ccggcaatgc taaaaggata
    24241 tattgacaga gtatggaata atgggctggc ttatggagat gggcacaaat taccattcaa
    24301 taaagttcgt tgggtggcgc tggttggagg agacaaagaa tcatttgtcc agatgggctg
    24361 ggaaaaaata taagcgatta tttaaaaaat atgtgcagtt atcttggtat tgaagatgcc
    24421 gatgtcactt tcttgtgtaa tacagtggta ttcgatgggg aagaacttca cgcgagctat
    24481 tatcagtcgt tattatctca ggtacgggat atggtagatg cactataaga tgtgttaaaa
    24541 acgctgtagc agaatgaagc gcggaataaa aaagcggcaa ctcaataaag ttgccgcttt
    24601 acggggaaat tagaacatta ccttatgacc gtactgctca agaatgcctt tcacgcgttc
    24661 catggtctct ttcttcggtg gtttaacacc gtcgagtttg tactcttcac ccattgccac
    24721 ccatttgtgt ttgcccagct cgtggtaggg gagaagctcg attttctcaa cgttgcccat
    24781 atcacgggta aattcaccga ggcgatgcgc tgaatcgtca tcgtcagacc agcctgggac
    24841 aacaacgtag cggatccaca ccttcacatt tttgttcgcc agatatttag cgaactccag
    24901 cgtgcggtgg ttggaaactc caaccagatt ttggtggatc tcgtcgttca tctgtttgag
    24961 atcgagcatt accaggtcgg ttacttccag cagttcatca atcaccggat cgtaacgacg
    25021 aacaaaaccg ttggtgtcca gacaggtatg aatgccttct tttttgcagg cgcggaacca
    25081 gtcacgaaca aactcagctt gcaggattgc ttcaccgccg gatgcggtaa cgccgccgcc
    25141 ggaagcgttc ataaagtggc gataggtcac cacttccttc atcaaatctt caacggtaac
    25201 ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga caatacaggc agcgcatcag
    25261 gcagccctgg aaaaaggtga taaagcgaat acccgggccg tctacggttc cacaggattc
    25321 aaaggagtga atgcgaccaa taactgactt ggtagccccc gccgcagcgg tattttctgt
    25381 tgtattacaa ttagttaaat tactcatatc gcttcaattg gctctagtta actctggttt
    25441 atggacgcat tatggacatt cctgacaccg ggttaagagt cacagcatct tgtaagaaat
    25501 ccggtgcaaa gtgtgcgtag gtcatagttt gctgaatgtt agaatgaccc aggatgcgct
    25561 gcaatgtgat tatgttacct ccattcatta taaaatgtgt ggcaaatgta tgcctcaaaa
    25621 catgcactgc ctgtccgtca ggtaaatcgg gttttacttc cctgagagcg ttgcgcactt
    25681 tgtagtaact ggcattaaaa agcctgcctg aatttttggt cttgatccgt ttaatcaggt
    25741 cctgcgaaac gggaattgtc ctgcgctttc cgtttttagt tttcataaac gtaaccatct
    25801 ggttaatgat gtgttcagct ttcaaattag acacttcact ccagcgtcca ccagtagaaa
    25861 ggcagaccag agttgcattt aactcatcgc catcaagcat ggataacagc cgcgtaatct
    25921 cttcactgga caaaaaagcc atttccgtaa cagcttcacg taaccgctta acctcacgga
    25981 acgggttgtg agagtgatat tcaccggcgt caattaactt ggtgaacatc ccgctcatta
    26041 ttgccagatg ccgatttacg ctggctggtt ttaggccatc gttcatcatt acaacgcgat
    26101 aatcagttat cgttttcttt gttagctggt ccgccctgga cactcccatc tcggcaaatt
    26161 tggcgattat tgtcgtcaaa cgccctcgtt caatatctcc acgctcatgt gattttccgt
    26221 gatatatcca ccatctgcct aacaactctg taagagttcg gcggtcggcc ggcttctcca
    26281 accactcttt gttgtggtag ttaaccagga catgacgttc gaatgcttga gcttcacctt
    26341 tagttttaaa tttccgcctg atacgttttc catctgcacc ctgcggtctg acgtccactt
    26401 cataacgacc atcatcgagc tttttaatag acataaagcc ctccgatgac gctgtttact
    26461 tctactactt gaaaattaat gcaattttct ttcgtacatt tactgcacac atatgctgaa
    26521 taaatcgtca gccagtcttt tggtctgagt ggtgcaaggt tgttgagtct tgcccaatgt
    26581 gcgcgagcgc cggggctatt tgtccgccag cgggatcagt ttcatcaaac atgaaccagt
    26641 cacggtactt gcgaaatctt tctggcttga aaaatttcat acctgcgtca aaagacatct
    26701 ttacttttcc ctgctcatat ccagcatagg tgttgtagtt aattccagtt aattcagcaa
    26761 cttgcttcct tgtcattctt tctgattccc gaataagtgc gagtttctct gcttgagatg
    26821 tgatttgtgt atttgacatg aattgtcgta tctcgtaatt tatgttgtat gcgacacgcc
    26881 agaacaacgc agagcggctt caaatagctc tgattgaatg gcaccaaagt tgaggatatc
    26941 aaaatgagta ttggatcaga aatgaataac gatgttggag aaaaagtatc tgatctcaca
    27001 aaaagtaaaa aatgtgacat caaacttgca gccgcaccgt cggatttgct ctcgaaagag
    27061 ggttttgctc tttacatcgg taagacgcct cgtgctgttg ctgaaatggc gaaagcaggc
    27121 aagttaccag ccttttatat gacggaccca ttaaagccgg gcggtcatgc tgagttatgg
    27181 attaatcgtc gtgagtggga caagtacgca gcccagctag ttgatgaagc tccgacagaa
    27241 tggcatgact ggaaaaatcg catttgttac agcaaatcaa gacatggccg tgcggcttaa
    27301 ggtggaaagg atgaacgagc ctcgttgtat tgctcagtta ttgcgtaacg aaagccccag
    27361 ggcgattgac ttcaccatca cccacgggaa ggggcgcaag ggaatcatta tccgtaccaa
    27421 aaaacagagt ccgttaaaaa aggctctgac ctttctgaaa agccggaggg tctggaaatg
    27481 acagtgatga cgcttaatct cgtcgaaaaa cagccagcag ctatgcgccg gataattggt
    27541 aagcatcttg ccgtccctcg ctggcaggag acatgcgatt attataatca gatgatggaa
    27601 cgcgaacggc taacggtttg ctttcatgcg cagttaaaac agcgtcacgc aacgatgcgt
    27661 tttgaagaaa tgaacgacgt cgaacgtgaa cgactggtat gtgcaattga tgaattgcgc
    27721 ggggcattct caaaacgccg tcaggttggc gcaagtgagt atgcatatat tagtttttta
    27781 acagtcagtc agcgtcgcac tttatttatg cacgcacgac tgacagaaaa agaatttaac
    27841 cagccatact ggcgaattaa tgaagagtca tgttactggc gtgatgcttt attccgtgca
    27901 ttacgtgaac tattcagtct gtttgagtat gcaccgacaa ttctgacgtc ggtaaaacca
    27961 gagcaatatc tgcattaaat aattaaccag agtttttaac gcacttaatc gtgcggggct
    28021 tctttttgcc tggagaaagt catgcataca gtttctgaaa atcagtgcgg taaatacgca
    28081 ttactgctgc aacaggccag aaccgaagca caggccgacg cggcgacgcg cttttcttct
    28141 catcttgatg ccatgattcg ccacatcaca aaggcggagt tatcccgcgt ggagatagtc
    28201 gagctgctca gtcaggagtc ggaaaaattt cacaatatcg gattgtctcg cggggaggtg
    28261 ctttgatgtc ctgttctcgt tcagttgtat tactgaataa cgccttaaaa atcgccgtta
    28321 tgaaaaatgg cgatttgtct cttattcaac ttggtcttga taaagaaaaa cgcgaaataa
    28381 ctgagtctgt tatcgcgatt tatcagaacg aattaaacct cctgtctgat gtggtcaatt
    28441 tacttgttaa acgcgctgtg tttcacaagc aaatctcctc cgtggatgaa ctgacgaaat
    28501 taacgacaga aatcgccagc tattgcgctg atgaatttaa aaaccttaac gacaaaagga
    28561 actggtaatg ccggacaacg tagattttat tcaggaacaa caggctgaat tactggagcg
    28621 ccagattaac gcggcaaggg taaaacattg cggtgcttct gcgctggttt gcgaagagtg
    28681 tgacgcgcc
//
LOCUS       DACAPB010000041        28027 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.041, whole genome shotgun sequence.
ACCESSION   DACAPB010000041 DACAPB010000000
VERSION     DACAPB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.041"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..662)
                     /gene="fkpA"
                     /locus_tag="IT904_001616"
     CDS             complement(<1..662)
                     /gene="fkpA"
                     /locus_tag="IT904_001616"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP3191411.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPP"
     gene            883..1101
                     /locus_tag="IT904_001617"
     CDS             883..1101
                     /locus_tag="IT904_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAP3191412.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(1150..1740)
                     /gene="slyD"
                     /locus_tag="IT904_001618"
     CDS             complement(1150..1740)
                     /gene="slyD"
                     /locus_tag="IT904_001618"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP3191413.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(1835..2035)
                     /locus_tag="IT904_001619"
     CDS             complement(1835..2035)
                     /locus_tag="IT904_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAP3191414.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(2045..3850)
                     /gene="kefB"
                     /locus_tag="IT904_001620"
     CDS             complement(2045..3850)
                     /gene="kefB"
                     /locus_tag="IT904_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAP3191415.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(3850..4401)
                     /gene="kefG"
                     /locus_tag="IT904_001621"
     CDS             complement(3850..4401)
                     /gene="kefG"
                     /locus_tag="IT904_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAP3191416.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            4532..6445
                     /locus_tag="IT904_001622"
     CDS             4532..6445
                     /locus_tag="IT904_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3191417.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            6445..7467
                     /locus_tag="IT904_001623"
     CDS             6445..7467
                     /locus_tag="IT904_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP3191418.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            7461..7679
                     /locus_tag="IT904_001624"
     CDS             7461..7679
                     /locus_tag="IT904_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAP3191419.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            7733..8602
                     /locus_tag="IT904_001625"
     CDS             7733..8602
                     /locus_tag="IT904_001625"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAP3191420.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(8657..9061)
                     /locus_tag="IT904_001626"
     CDS             complement(8657..9061)
                     /locus_tag="IT904_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAP3191421.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVGA"
     gene            9363..9995
                     /gene="crp"
                     /locus_tag="IT904_001627"
     CDS             9363..9995
                     /gene="crp"
                     /locus_tag="IT904_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAP3191422.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            10046..12136
                     /locus_tag="IT904_001628"
     CDS             10046..12136
                     /locus_tag="IT904_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191423.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12203..13423)
                     /gene="argD/dapC"
                     /locus_tag="IT904_001629"
     CDS             complement(12203..13423)
                     /gene="argD/dapC"
                     /locus_tag="IT904_001629"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAP3191424.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGHKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGSKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(13509..14072)
                     /gene="pabA"
                     /locus_tag="IT904_001630"
     CDS             complement(13509..14072)
                     /gene="pabA"
                     /locus_tag="IT904_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAP3191425.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSEIREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(14104..14706)
                     /locus_tag="IT904_001631"
     CDS             complement(14104..14706)
                     /locus_tag="IT904_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAP3191426.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(14696..14863)
                     /locus_tag="IT904_001632"
     CDS             complement(14696..14863)
                     /locus_tag="IT904_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAP3191427.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(14968..15540)
                     /gene="ppiA"
                     /locus_tag="IT904_001633"
                     /gene_synonym="rotA"
     CDS             complement(14968..15540)
                     /gene="ppiA"
                     /locus_tag="IT904_001633"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAP3191428.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            15811..16992
                     /gene="tsgA"
                     /locus_tag="IT904_001634"
     CDS             15811..16992
                     /gene="tsgA"
                     /locus_tag="IT904_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAP3191429.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            17255..19798
                     /locus_tag="IT904_001635"
     CDS             17255..19798
                     /locus_tag="IT904_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAP3191430.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILSSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            19795..20121
                     /gene="nirD"
                     /locus_tag="IT904_001636"
     CDS             19795..20121
                     /gene="nirD"
                     /locus_tag="IT904_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAP3191431.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            20248..21054
                     /gene="nirC"
                     /locus_tag="IT904_001637"
     CDS             20248..21054
                     /gene="nirC"
                     /locus_tag="IT904_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAP3191432.1"
                     /translation="MFTDTINKSAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            21073..22446
                     /gene="cobA"
                     /locus_tag="IT904_001638"
     CDS             21073..22446
                     /gene="cobA"
                     /locus_tag="IT904_001638"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP3191433.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            22693..22860
                     /locus_tag="IT904_001639"
     CDS             22693..22860
                     /locus_tag="IT904_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAP3191434.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            23104..24492
                     /gene="frlA"
                     /locus_tag="IT904_001640"
     CDS             23104..24492
                     /gene="frlA"
                     /locus_tag="IT904_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HAP3191435.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            24513..25535
                     /gene="frlB"
                     /locus_tag="IT904_001641"
     CDS             24513..25535
                     /gene="frlB"
                     /locus_tag="IT904_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAP3191436.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            25585..26415
                     /gene="frlC"
                     /locus_tag="IT904_001642"
     CDS             25585..26415
                     /gene="frlC"
                     /locus_tag="IT904_001642"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAP3191437.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            26412..27197
                     /gene="frlD"
                     /locus_tag="IT904_001643"
     CDS             26412..27197
                     /gene="frlD"
                     /locus_tag="IT904_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP3191438.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAIAQGTACAAKTIQYHGAW"
     gene            27297..>28027
                     /gene="phoB"
                     /locus_tag="IT904_001644"
     CDS             27297..>28027
                     /gene="phoB"
                     /locus_tag="IT904_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAP3191439.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         6685 a         7561 c         7197 g         6584 t
ORIGIN      
        1 ggtggaataa ccagtttgat cttaccgcct ttcttgatgt tcttcagacc ttctgtccaa
       61 cccgggataa caccgtccag acggaaagaa agcggttcac cacgggtgta agagttgtcg
      121 aactctttac cgtcgatcag cgtacctttg tagttcacta caacagtatc gctgtctttc
      181 ggtgcttcgc ctttaccggc ttctactacc tgataaacca gaccagttga agaggttttc
      241 acacctttct ctttggcaaa tttctcgcgg tactctttac cttttgcttc gttatcagcc
      301 gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga
      361 gtctgttcga tctcttggtc ggagagtttg ctcttatcag caaatgcatc ctgaacacca
      421 gcgatcagct gatctttatc cagtttgatg cccagttttt cttgttcttt tagagagttt
      481 tccatgtaac gacccagcga ggcacccagt gcataagctg atttctgatc gtcatttttg
      541 aacgctgctt tgctgtcagc agttgtagca ggttttgcag cttcagcagc aaaagtgatt
      601 ggtgcatgca gggcaacggc cattgtggtc gccagcagcg ttactttaaa cagtgatttc
      661 atccatatct ccaggatcgg ggcatctcac cccagggtta actattatca gaagggtact
      721 ataaagcgtt gtcgaacaaa tctacataca gacacgccct attatcatct attttcagac
      781 tctttttgtt taaattagtt tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt
      841 taagtagaat ccgcggcgac cattcgacaa aagaggtgaa tcatgcagga tttatcattg
      901 gaagcacgcc tggcagagct ggagagccga ctggcttttc aggaaatcac cattgaagaa
      961 ctgaacgtca cggtgaccgc tcatgaaatg gagatggcga aactgcgcga tcatctgcgt
     1021 ctgctgaccg agaagttaaa agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa
     1081 acgccaccgc cacattattg aggcgtaaaa aaagcgggga ttccccgctt ttttgtcact
     1141 ttttcggtat tagtggcaac cgcaaccgcc gttgccttta ccgccacagc agccttcgcc
     1201 accgtgttca tgaccgtgat cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc
     1261 gtggtgatga tcgtgcgcgc cgtgaacgtg accatgagcc agttcttctt cggtcgcttc
     1321 acgaatcgcc acaacctcaa cgttgaattt caggttctga ccggccagca tgtggttacc
     1381 atcaaccact acgtgatcgt cttcaaccgc agtgatttca accggtaccg gaccctggtc
     1441 ggtttcagcc aggaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt
     1501 aggaacacgt tgcaccaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc
     1561 gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag
     1621 ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac
     1681 accgtcttct gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat
     1741 gatatctcct gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg
     1801 tactcactag cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca
     1861 cttgctcatc tttgcgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc
     1921 cgcacttaac gcattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct
     1981 gtgcctgaca ggccgggcat tttgcgcccg caataaaacg ttttcggatt gccatcttta
     2041 ccctctactc aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct
     2101 ggaaaatctc ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acagtatcgg
     2161 catgcattgg gatgagctct cgcagcatcc gcatatccag gcggcgaaaa tgcagttgcg
     2221 cccgctgcgc ctgatgcgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta
     2281 acgcactgga gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg
     2341 cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata
     2401 tttccaccag cttcatggtg tcttccggtt cgttacaggt gatcacgata gactcagcgg
     2461 cctctgcacc cgcagaacgt aaaagatcga cctgcgtggc gtcgccgtaa taaactttgt
     2521 agccgtattt acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca
     2581 ttttatttgc catcagcaaa cgaccaatca cctgaccaaa acgcccgaag cccacgacaa
     2641 tgacctgggg tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa
     2701 actggcggga tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa
     2761 gcgtcaccgt caccagcaac agcgccatct ggtcgccctg gaataagcgt tgcgaagaag
     2821 cggtagaaaa gaggacaaag gcaaactcgc cgccctgact caacacgcca gcaaactgca
     2881 tccgctctga gctacgcacg ccatacaatc gcgccagcag atacaacacg agaattttca
     2941 ccgccaccag cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga
     3001 gattgagcga catgccgaca gagataaaga acaaaccgag cagcaagcct ttaaagggat
     3061 cgatagccgt ttccagttca tggcgatatt cactttccgc cagcagcacg cccgcaataa
     3121 atgtaccgag cgccatcgac agccccagcg catccataaa caatgcggaa cccaacacca
     3181 gcagcagcgt cgcggcggtg aacacttccc gcacgccaga agctgcaata aagcggaata
     3241 ccggacgcag taaatagcgc ccaccaatca gcatgccgac aaacgccagc accttcatgc
     3301 cgatcttcat ccagtcgaaa tgttcgtctg ccgaccccgc caacaacggc actaacgcca
     3361 gtgccgggat caccgccaga tcctgaaaca gcagtaccga gaaacctaac tggccggatt
     3421 cgctgcgatt catccctttc tcacgcatca attgcaacgc cattgcagtt gaagacatcg
     3481 caaggccaat gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc
     3541 ccgccagcaa cgccgcgctt aacagcacct gtgccgcgcc tacgccaaaa atcgaacgcc
     3601 gcagttgcca aagtttggag ggattcaact caaggccgat gataaacatc aggaatacca
     3661 cgccgagttc cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agcccccacg
     3721 ggccaattgc aatccctgcc agcaaatatc ccaacacagc gccaataccc agccgcgatg
     3781 ccagcggcac cgcagccacc gccgcgaaga gaaacagcac tcctgcgagt aaaaaatcgg
     3841 aaccttccat cagcggcctc ctggagacag cggatttgcc agccagtcac cgtaggctct
     3901 ggcgtggctc gccagctcct gtgcgctttg ccgtctcgcc cagtaaataa tgatgggact
     3961 taaccaatgc atccggcaca tgcccgccgc cagttcaaag gggcgcagca catcgctcat
     4021 cgggtagcga ttcagcgcgt cataacggta agcactttcc ggctcgccgg tggtaatcac
     4081 gctacgccag tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacgact
     4141 taatacccgg tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg
     4201 ctgaaagaca atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa
     4261 aaaatcggga tagtgcgcgt aaaggtcgtg cacggtaaca ttgctgagct gcgtggccgg
     4321 tttaagcagt acccggtttg ccaccgagtc ctgagattcc ggatgggcat acagcagcaa
     4381 aactttcgct ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt
     4441 tttgggctac cattgcgccc ggtgcggcag ctcgcccata cattacatta tcataatgat
     4501 aagttaacat agtctgaaca tacggcacct tatgattgtt ttctcctcgt tacaaattcg
     4561 tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc atcaaccctg ggcagaaagt
     4621 cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga
     4681 aatcagcgcc gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa
     4741 tcaggaaacg ccggcgttac cgcaagcggc gctggaatat gtcattgacg gcgaccgtga
     4801 atatcgtcaa ctagaagcgc agctacacga cgccaacgaa cgtaacgacg ggcacgccat
     4861 tgcgaccatt catggcaagc tggatgctat tgacgcatgg agtattcgct cccgtgctgc
     4921 cagcctgctg cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt
     4981 ctccggtggc tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt
     5041 gctgctgctc gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa
     5101 atggttgaag agctatcagg gcacgctgat cctgatctct cacgaccgcg acttcctcga
     5161 tccgatcgtt gataaaatta ttcatatcga acaacaaagc atgttcgagt acaccggcaa
     5221 ctacagttcg tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtatga
     5281 aagccagcag gaacgcgtag cgcatctgca aagttatatc gaccgtttcc gtgccaaagc
     5341 caccaaagcg aagcaggccc agagccgcat taagatgctc gagcgtatgg agctgattgc
     5401 ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc gcgccggaaa gcctgccaaa
     5461 tccgttactg aagatggaaa aagtcagcgc gggctatggc gatcgcatta ttctcgactc
     5521 gattaaactg aacctggtgc ccggctcgcg cattggtctg ttaggccgca acggcgcggg
     5581 taaatcgaca ttaatcaaac tgttagccgg tgaacttgcg ccagtcagcg gtgaaattgg
     5641 tctggcgaaa gggatcaagc tcggctactt cgcccagcat caacttgaat acctgcgcgc
     5701 cgacgaatca cctattcaac atctggcacg tttagcgccg caggagctgg aacaaaaact
     5761 gcgtgactac ctcggcggct ttggtttcca gggcgataaa gtaaccgaag aaacgcgccg
     5821 cttctcaggt ggggaaaaag cccgcctggt gctggcatta atcgtctggc agcgtccgaa
     5881 tctgctgctg ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcactcac
     5941 cgaagcatta atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct
     6001 gcgttccacc actgacgatc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg
     6061 cgatctggaa gattatcaac agtggttgag cgacgtacaa aagcaggaaa accaggccga
     6121 cgaagcgcca aaagagaacg cgaacagcgc ccaggcacgt aaagatcaga agcgccggga
     6181 agcggagctg cgtgcgcaaa cccagccact gcgtaaagag attgcccgtc tggaaaaaga
     6241 gatggagaag ctgaacgcgc aactggcgca ggcggaagag aaactcggcg acagcgaact
     6301 gtatgaccag agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa
     6361 atccggcctg gaagagtgcg aaatggcgtg gctggaagcc caggagcagc ttgagcagat
     6421 gttgctggaa ggccaaagca actgatggcg cagataacga cgaccgatgc caatgaattc
     6481 agcagcagtg ctgaattcac ccctatgcgc ggctttagca attgtcatct gcaaaccatg
     6541 ctgccgcgtc tgtttcgtcg caaggtgaaa ttcaccccgt actggcagcg gctggagttg
     6601 cccgacggcg attttgtcga tctcgcgtgg agtgaagccc cagcacaggc gcggcataaa
     6661 ccgcgtttag tggtatttca cgggctggaa ggcagtctca atagccctta cgcccacggt
     6721 ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc
     6781 agcggtgaac caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt
     6841 tggtttttac gctggctgca acgcgaattt ggtcatgcgc caacggctgc cgtcggctat
     6901 tcgctcggcg gtaatatgct ggcctgtttg ctggcaaagg aaggcaatga tctcccggtt
     6961 gatgcggcgg tgattgtctc tgcgccgttt atgctggaag cctgtagcta tcatatggaa
     7021 aagggctttt cccgcgttta tcagcgttac ttgctgaacc tgttaaaagc caatgccgcg
     7081 cgcaagctgg cagcctaccc cggaacgctg ccgattaatc tcgcgcagtt aaaatcggta
     7141 cgtcgcatcc gtgaatttga cgatctcatc accgccagaa ttcacggcta cgccgacgct
     7201 atcgactatt atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaaccgacg
     7261 ctgattattc acgccaaaga cgatccgttt atggatcatc aggtgatccc gaaaccggaa
     7321 agtctccccc cgcaggtgga gtatcaactg actgaacatg gcggtcatgt tggctttatt
     7381 ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca
     7441 acgtatctgg aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg
     7501 aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta
     7561 cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtgctgg
     7621 tatggtcgga gctgcacgaa acggtcaata ttatgccgcg cagtcagttt cgcgaataac
     7681 ctgcaaccgt cggcggacga gataataatg atagctaagt catggagtta ctatgtctgc
     7741 caaacatccg gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct
     7801 cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca gctgaggtgg aaggcgacag
     7861 ttttcaccgt tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg
     7921 gcggcatatc agctacttcg gccccgaagc gaacgacttc ggcctgctgg aacaaacctt
     7981 cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgatga
     8041 agccgtaccg tggaatcagg taccgggaac attcaccccc tggcaacctt taccagaacc
     8101 cacagatgta ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt
     8161 tgcgcagcat gtggacttac tggtcggcgt ggtgcctatc gttaaccttg agtggattca
     8221 aaaactgatc cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcagt
     8281 agtgcgttca atggaagact atatcaacta catcacaccg cagttttccc gcacccatct
     8341 taacttccag cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc
     8401 gtcgctggat gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc
     8461 ctggctgctg gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtgcc
     8521 cggcggcaaa atgggtctgg caatggaatt aattatgctg ccgctggtgc aacgattgat
     8581 ggaaggaaag aaaatcgagt aactctgcta ttacgccgga taaaatacta tccggcttca
     8641 caacgggata gttaaatcac gcgccaacca cttcatacga gtgagtaata ttcaccgctt
     8701 tttccagcat caacgccact gaacaatatt tctcggcaga gagatcaacc gcacgcgcaa
     8761 ccgctgcgtc tttcaggtcg cgaccggtga cgataaaatg cagattaatg tgcgtaaaca
     8821 ggcgtggtgc ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct
     8881 gacgcccttt ttgcaggatc gaaaccacat cgatggcact gcaaccaccc gccgccatca
     8941 acaccatttc catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctggt
     9001 gaccagaggc agattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca
     9061 tttttgctac tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc
     9121 aacggaaggc gacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta
     9181 tgctaaaaca gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg
     9241 tcacattacc gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg
     9301 caatccagag acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc
     9361 gcatggtgct tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc
     9421 acattcataa gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc
     9481 tgtactacat cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa
     9541 tgatcctctc ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg
     9601 gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt
     9661 acaaaaaatt tcgccaattg attcaggtaa acccggacat tctgatgcgt ctgtctgcac
     9721 agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg
     9781 tgacgggccg cattgcacag actctgctga atctggcaaa acaaccagac gctatgactc
     9841 acccggacgg tatgcaaatc aaaattaccc gtcaggaaat cggtcagatt gtcggctgtt
     9901 ctcgtgaaac cgtgggacgc attctgaaga tgcttgaaga tcagaacctg atctccgcac
     9961 acggtaaaac catcgtcgtt tacggcactc gttaatcccg tcggagaggc gcgttacctg
    10021 gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa
    10081 ctatgcactt cgacaaacgc tggtgctatg tttgcccgtg gccgttgggt taatgcttgg
    10141 cgaattacga ttcggtctgc tcttctccct cgttcctgcc tgttgcaata ttgcgggcct
    10201 tgatacgcct cataaacgtt ttttcaaacg cttaatcatt ggtgcgtcgc tgtttgccac
    10261 ctgtagctta ctgacacagc tgctactggc aaaagatgtt cccctgccct ttttgctgac
    10321 cggattaacg ctggtacttg gcgtcactgc tgagctgggg ccattgcacg caaaattgct
    10381 tcctgcatcg ctgctcgccg ccatttttac cctcagtttg gcgggataca tgccggtctg
    10441 ggaaccgttg ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg
    10501 gttctggatc tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgtgaact
    10561 ggcagattat tgtgaagcca aatacagcct gcttacccag cacaccgacc ctgaaaaagc
    10621 gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat ctaattaccc agtgctatca
    10681 gcaaatgcat atgctttccg cgcaaaataa tactgactac aagcggatgc tgcgtatttt
    10741 ccaggaggcg ctggatttac aggaacatat ttcggtcagt ttgcatcagc cggaagaggt
    10801 gcaaaagctg gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt
    10861 cgccgctcgc ctgcgcgtgc tggctgatga cattctttac catcgcctgc cgacgcgttt
    10921 tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc
    10981 ggttgggcaa ttctgctatt ggcacttcag ccgcatcgcc cgcgtgctgc gcacccaaaa
    11041 accgctctat gcgcgtgact tactggccga taaacagcgg cgaatgccgt tacttccggc
    11101 gctgaaaagt tatctgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt
    11161 aatgttaagc gttgccagcc tgatgggcac cgcgctgcat ctgccgaagt cgtactggat
    11221 cctgatgacg gtattgctgg tgacgcaaaa tggctatggt gcaacccgtc tgaggattgt
    11281 gaatcgctcc gtgggaaccg tggtcgggtt aatcattgcg ggcgtggcgc tgcactttaa
    11341 aattcccgaa ggttacaccc tgaccttgat gctgattacc acccttgcca gctacctgat
    11401 attgcgcaaa aactacggct gggcgacggt cggttttact attaccgcag tgtataccct
    11461 gcaactgttg tggctgaacg gtgagcagta catccttccg cgtcttatcg ataccattat
    11521 tggttgttta attgctttcg gtggtaccgt ctggctgtgg ccgcagtggc agagcgggtt
    11581 attgcgtaaa aacgcccatg acgctttaga agcctatcag gaagcgattc gcttgattct
    11641 gagcgaggat ccgcaaccta cgccactggc ctggcagcga atgcgggtaa atcaggcaca
    11701 taacactctg tataactcat tgaatcaggc gatgcaggaa ccagcgttca acagccatta
    11761 tctggcagat atgaaactgt gggtaacgca cagtcagttt attgttgaac atattaatgc
    11821 catgaccacg ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt
    11881 acagtcttgt gaaatcgcca ttcagcgttg tcagcagcga ctggagtatg acgaaccggg
    11941 tagttctggc gatgccaata tcatggatgc gccggagatg cagccgcacg aaggcgcggc
    12001 aggtacgctg gagcagcatt tacagcgggt tattggtcat ctgaacacca tgcacaccat
    12061 ttcgtcgatg gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg
    12121 ggattcgaag gcgtaatgca ggctaaacca tcggataagg cattcacgcc gcatccgaca
    12181 tcttttgcct gatgcgtctt atttacgccc caaccacctt cgccaccgcg tgggcgaaac
    12241 gttgcatccc ttcatcgata tccgcatctt ccaccaccag cgacggtgca aaacgcatca
    12301 catccggtcc ggcattcagc accattacgc cagcctctgc gcccgcatac aggaaatcac
    12361 gcgcctgacc tttgtactgt ggtttcagct ccgcgccaat taacagcccc ataccgcgaa
    12421 tatcgctaaa cacatcgtac tgctgatcga tcttctgcaa atggtcaaca aaacgctggc
    12481 gtttcgcctg aatgccttcc agcacttcag gggtattaat gatatcaaac gccgcgcccg
    12541 ctactgcaca ggccagagga ttaccgccgt aggtggaacc gtgagaacca ggatgaaacg
    12601 cagaagcaat ttccgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt
    12661 tcgcagaagt cagaatatcc ggcgtcacgc cgtagtgcat gtaagcaaac aaatcgccag
    12721 tccgccccat cccgcactgt acttcatcaa acaccaataa tgcctgatgt tgatcgcaca
    12781 actcacgcaa gccctgcaaa aactctggcg tcgctgccgt cacaccgccc tcgccctgga
    12841 tcggctcaac caccactgca caggtgtgat catccatcac cgctttcact gcgtggagat
    12901 cgttaaaggg aacgtggatg atgtctgccg gttttgaccc aaagccgtcg gaatattttg
    12961 gctgcccacc caccgaaacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa
    13021 taattttggt tttgaacggg ctatgacgca cacaggcgta atggcgtgcc agtttaaagg
    13081 cggtttcgtt agcttccgtg ccggagttca taaacaccac gcgttcggca aacgtcgcct
    13141 caatcagttt atgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc
    13201 acagagtttc gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa tggcccaacg
    13261 ccgtaactgc aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc
    13321 ggctgccctg accttttacc ggaataaact ctgccggtgc ataaatcggc aggatcactt
    13381 catcgaaagt cgcgcgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt
    13441 acgcataaat aaatttgaaa ttataatcac aaaatatgca taaaaaatca ctgaatggca
    13501 atcagaaatc agcgatgcag gaaattagcc agcagttgat gtccttgttc gctaagaata
    13561 ctttctggat ggaactgcac accttccaga tcccactggc gatggcgaat ccccataatc
    13621 tcgcggattt cgctccaggc cgtcacgtca aagcacgctg gtaatgagtc aggttccacc
    13681 accagcgaat ggtagcgtgt cacggtaagt ggatttgcca gcccccgaaa tacgccctca
    13741 ccgttatgtg taatcggcga ggttttgccg tgcatgacct ttgcggcgcg cacaacttta
    13801 ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc
    13861 ccggcatagt ggcgaataac gtcaagggag atcccggctt catctggcgt acaggggcca
    13921 ggtgagatga caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg
    13981 ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta
    14041 aaagaatcgt agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccacgct
    14101 attttactca gattccccgg cttcgcttac cactttgcta aatatcatct gcagcgcggt
    14161 gagatccccc attgccccac tctgatttgc ctgattccag gcttcttttt cgataccctg
    14221 ccagctcagt tgatatccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag
    14281 tccgcttccc acacggaagg gatgcagcac gttgatttca caatagtaat gcgccagccg
    14341 ctcgacgaac ttcgctttct ccaggccaac cagataacct tcttcctcca gatcctgcat
    14401 cagggcattg ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata
    14461 aatatcaact tcacgcagtt gccccgccca gtcgaaaata tcctgataca gctggcgatg
    14521 gatagtgcgc aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg
    14581 cagcgccgtc atttcgtaag cggcctgttc cagccgctgc tgctggtgga tgttcagccg
    14641 gttacgcatg atatcaaggc caggataaag atacggatcg cgcccttcgc cgaatttatc
    14701 gctcatagtg cctcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga
    14761 ctaaaggcat ctcgacgcct tcaagacggc gactggcctg gaaattacga ttacgctgaa
    14821 gctcccagag acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac
    14881 gtgtccattt gccacaagta taagcggcaa atccgggtta cgccgggaga gcagaagcgc
    14941 gggcaagatt gcccgcgcga aaaatcatta cggcaggact ttagcggaaa ggataactac
    15001 cggttttgac ggcacattct ggtacggacc aacgtcatga gtcggcacct gggaaatctt
    15061 atcggcaacg tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg
    15121 accatggtca aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc
    15181 tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcggc
    15241 ttcatttttg attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc
    15301 ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt
    15361 cacataatcg acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc
    15421 gatgttacca gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc
    15481 gggagaaaga gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat
    15541 gagatttcct taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc
    15601 aagaacaagg ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat
    15661 cactttttat tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat
    15721 aaaacattta aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta
    15781 tgccatttta tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca
    15841 tggattagct ttctctccta cgcactgact ggtgcgttgg ttattgtcac cgggatggtg
    15901 atgggaaata tcgccgatta tttcaatctg cctgtttcca gtatgagtaa caccttcacc
    15961 ttcctcaacg ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc
    16021 ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg
    16081 atgttcagcc acagcctggc gctgttctcg acggcgatgt tcattctcgg ggtggtcagc
    16141 ggcattacta tgtcgattgg tacattcctg ataacacaaa tgtatgaagg gcgtcagcgc
    16201 ggttcccgcc tgttatttac tgactccttc ttcagtatgg ctgggatgat tttcccaatg
    16261 atcgccgcgt ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg
    16321 ctggtgtatg tcgctatttt tattctgacc ttcggctgtg agttcccggc actgggcaaa
    16381 catgcgccaa aaacggatgc tccggtagag aaagaaaagt gggggatcgg cgtactgttt
    16441 ctctctgttg cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgcct
    16501 gagtatgcca aaggcctggg catgagcctg aacgacgcgg gcacgctggt gagtaacttc
    16561 tggatgtcat acatggtcgg catgtgggcg ttcagcttta ttcttcgctt ctttgatttg
    16621 caacgcattc tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc
    16681 ggaacaccag cacatatggc gtggtcaatt ctcgctctgg gcttcttctc cagcgcgatc
    16741 tataccacca tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt
    16801 aactttgtcc tgacctgcgg aaccatcggt actatgttga cctttgtggt taccggcccg
    16861 attgttgaac atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc
    16921 gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg
    16981 acctctcatt aattgctcat gccggacggc actatcgtcg tccggccttt tcctctctta
    17041 cccccgctac gtgcatctat ttctataaac ccgttcattt tgtctatttt ttgcacaaac
    17101 atgaaatgtc agacaattcc gtgacttaag aaaatttata caaatcagca atatacccct
    17161 taaggagtat ataaaggtga atttgattta catcaataag cggggttgct gaatcgttaa
    17221 ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc aaagtcagac tcgcaattat
    17281 cggtaacggt atggtcggcc atcgctttat cgaagatctt cttgataaat ctgatgcggc
    17341 caactttgat attaccgttt tctgtgaaga accgcgcatc gcttatgacc gcgtacacct
    17401 ctcgtcttac ttctctcacc acaccgccga agagctgtcg ctggtgcgcg aaggcttcta
    17461 cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct atcaccatca accgtcagga
    17521 gaaggtgatt cactccagcg ccgggcgcac cgttttttat gacaagctga tcatggcgac
    17581 cggttcctac ccgtggatcc cgccaatcaa aggttctgat actcaggact gctttgtcta
    17641 tcgcactatt gaagacctca acgccattga atcctgcgcc cgtcgcagca aacgcggtgc
    17701 cgttgttggt ggcggcctgt taggtctgga agccgctggc gcgctgaaaa acttaggtat
    17761 tgaaacccac gttatcgaat ttgcccctat gctgatggcg gaacagcttg atcagatggg
    17821 tggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg cgcgttcaca ccagcaaaaa
    17881 cacccttgag attgtgcagg aaggtgtcga agcgcgtaaa accatgcgtt ttgccgacgg
    17941 cagcgaattg gaagtcgact ttatcgtctt ctctaccggt atccgtccgc gcgataagct
    18001 ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt attgtcatta atgattcctg
    18061 ccagacttcc gatccggata tctacgccat cggtgaatgc gcaagctgga acaaccgtgt
    18121 atttggtctg gtagcaccag gctacaaaat ggcgcaggtc gccgttgacc atattctcag
    18181 tagcgaaaac gcctttgaag gtgctgacct tagcgccaag ctgaaactgc tgggcgtaga
    18241 cgtaggcggt attggtgatg cgcacggtcg cacgcctggc gcacgtagct atgtttacct
    18301 cgacgaaagc aaagagatct acaaacgcct gattgtcagc gaagacaaca aaaccctgct
    18361 cggtgcggta ctggtgggcg ataccagcga ctacggcaac ctgctgcaac tggtgctgaa
    18421 cgctatcgag ctgccggaaa acccggattc tctgattctg ccggcgcact cgggtagcgg
    18481 taagccgtct atcggtgttg ataaactgcc ggacagcgcg caaatctgct cctgcttcga
    18541 cgtcaccaaa ggtgatctga ttgctgccat caacaaaggc tgccacaccg ttgcggcgct
    18601 gaaagccgaa accaaagcgg gtactggctg cggtggctgt atcccgctgg tcactcaggt
    18661 actgaacgcg gaactggcga aacagggcat cgaagttaac aacaacctgt gcgaacactt
    18721 tgcttattcg cgtcaggaac tgttccattt gatccgcgtt gaaggcatta aaaccttcga
    18781 agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa gtttgtaaac cgaccgtcgg
    18841 ttcgctgctg gcctcctgct ggaacgaata cattctgaag ccggaacata ctccgctgca
    18901 ggattctaac gacaacttcc tcgctaacat ccagaaagac ggcacctact cggtgatccc
    18961 gcgctctccg ggcggtgaaa tcaccccgga agggctgatg gcagtgggtc gtatcgcgcg
    19021 tgaatttaat ctctacacca aaatcaccgg ttcccagcgc ctggcgatgt ttggcgcaca
    19081 gaaagacgat ctgccggaga tctggcgtca gctgattgaa gccggcttcg aaaccggtca
    19141 tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg ggtagcacct ggtgccgcta
    19201 cggcgttggc gacagcgtcg gcctcggcgt ggaactggaa aaccgctaca aaggcatccg
    19261 tacgccgcac aaaatgaagt tcggtgtctc cggctgtacc cgtgaatgtt cagaagctca
    19321 gggtaaagac gtgggtatta tcgccactga aaaaggctgg aacttgtatg tttgcggtaa
    19381 cggcggcatg aaaccgcgtc atgcggatct gctggcagcg gatatcgatc gcgaaacgct
    19441 gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt accgccgaca aactgacgcg
    19501 taccgcgccg tggttagaaa acctcgaagg cggcatcgat tatctgaaag cggtgatcat
    19561 tgacgacaaa ctggggctga acgctcatct ggaagaagag atggcgcgcc tgcgtgaagc
    19621 ggtagtgtgt gagtggactg aaacggtcaa tacgccgtct gcgcagactc gcttcaaaca
    19681 cttcatcaac agcgacaagc gtgacccgaa cgtgcagatg gtgccagagc gcgaacagca
    19741 ccgtccggca acgccgtatg aacgtatccc ggtaactctg gtggaggaca acgcatgagc
    19801 cagtggaaag acatctgcaa aatcgatgac atcctgcctg aaaccggcgt ctgcgcgctg
    19861 ttaggtgacg agcaggtagc gattttccgc ccgtatcaca gcgaccaggt gtttgcgatc
    19921 agcaacatcg atccgttctt cgagtccagc gtgctatcac gcgggctgat tgcggaacac
    19981 cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc gttttcgctt aagtgatggc
    20041 ttgtgcatgg aagacgaaca gttttccgtc aaacattacg aagcacgagt gaaagacggc
    20101 gtggtgcagc tgcgcggtta atgttttaac gggaggcgca atgcctcccc tttttgcatg
    20161 gtcctgtaat gaccttcggt atattgcagg acatttttta aactttttgt tttatttttt
    20221 tgtttttatt ttttaaagga taatcaaatg tttacagaca ctattaataa gtctgcggct
    20281 aacgctgcgc gcattgcacg cctgtcggca aataacccgc tcggattttg ggtcagctcc
    20341 gccatggcgg gcgcgtatgt gggtcttggg atcatcttga ttttcacgct cggtaatttg
    20401 ctcgacccgt ccgtacgtcc gctggtgatg ggcgcgacct ttggtatcgc cttaacgctg
    20461 gtgattatcg ccggttctga actgttcacc ggacacacca tgttcctcac ctttggggta
    20521 aaagcgggca gcatcagcca cgggcaaatg tgggcaatcc tgccgcaaac ctggctgggt
    20581 aatctggtcg gttccgtctt cgttgccatg ctctatagct ggggtggcgg tagcctgctg
    20641 ccggtagata ccagcatcgt tcactccgtc gcgctggcta aaaccactgc accggcaatg
    20701 gtactgttct tcaaaggtgc attgtgtaac tggctggttt gcctggctat ctggatggcg
    20761 ctgcgcactg aaggggcggc gaaatttatc gctatctggt ggtgtctgct ggcgtttatt
    20821 gcatccggtt atgagcactc catcgctaac atgacgctgt tcgcgctctc ctggttcggc
    20881 aaccacagcg aagcctacac gctggcgggt attggtcata acctgctgtg ggtgacgctg
    20941 ggtaatactt tatcaggtgc cgtattcatg ggattgggtt attggtatgc tacgccgaaa
    21001 gcgaatcgtc cggttgcgga caaatttaat caaactgaaa cggctgccgg ttaattacta
    21061 aggggttttt acgtggatca tttgcctata ttttgccaat tacgcgatcg cgactgtctg
    21121 attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt tgctgttaga cgcaggcgct
    21181 cgcttaacgg tgaatgcatt agcgtttatt ccacagttca ccgcatgggc agatgcaggc
    21241 atgttaaccc tcgtcgaagg gccatttgat gaaagccttc tcgacacctg ctggctggcg
    21301 attgcagcga cggatgatga cgcgcttaac cagcgcgtca gcgaagccgc tgaagctcgt
    21361 cgcatcttct gtaacgtggt cgatgcgccg aaagccgcca gctttattat gccgtcgatt
    21421 attgaccgct caccgctcat ggtagcggtc tcctctggcg gcacctctcc ggttctggca
    21481 cgcctgttgc gcgaaaaact tgaatcactg ctgccgttac atctgggcca ggtagcgaaa
    21541 tacgccgggc aattacgcgg gcgagtgaaa caacagttcg ccacgatggg tgagcgtcgc
    21601 cgtttctggg agaaattgtt cgttaacgac cgcctggcgc agtcgctggc aaacaacgat
    21661 cagaaagcca ttactgaaac caccgaacag ttaatcaacg aaccgctcga ccatcgcggt
    21721 gaagtggtgc tggttggtgc aggtccgggc gatgccgggc tgctgacact gaaaggactg
    21781 caacaaattc agcaggcaga tgtggtggtc tacgaccgtc tggtttctga cgatattatg
    21841 aatctggtac gccgcgatgc ggaccgtgtt ttcgtcggca aacgcgcggg ataccactgc
    21901 gtaccccagg aagagattaa ccagatcctg ctgcgggaag cgcaaaaagg caaacgcgtg
    21961 gtgcggctga aaggtggcga tccgtttatt tttggccgtg gtggcgaaga gctggaaaca
    22021 ctgtgcaacg cgggtattcc gttctcggtg gttccgggta ttaccgcagc ttctggttgc
    22081 tctgcctatt cgggtattcc actcacgcat cgcgattatg cccagagcgt acgcttaatt
    22141 accggacact taaaaaccgg tggcgagctg gactgggaaa acctggcggc agaaaaacag
    22201 acgctggtgt tctatatggg gttgaatcag gccgcgacta ttcagcaaaa gctgattgaa
    22261 cacggaatgc caggcgaaat gccggtggca attgtcgaaa acggtacggc agtcacgcag
    22321 cgcgtgattg acggtacgct cacacagctg ggagaactgg cgcagcaaat gaacagtcca
    22381 tcgctaatta ttattggtcg ggttgttggc ctgcgcgata aactgaactg gttctccaac
    22441 cattaattta acccgggcca gagaattctg gccttcttaa tgactctttt tatatattca
    22501 gcaaataaaa cataacccct taataaatat tatctgctaa tgggttatat tcaccgttac
    22561 agcgacttac aaaattaagt cattcgctca ctgacgccaa ttaaatattc ctgtatcgtt
    22621 cttaacgagc agtctgctta tattcatata atcaatgaat attaattaat atataataca
    22681 tagggaatgt aaatgaacaa atttattaaa gttgcactgg taggtgcagt actggctacg
    22741 ttaactgcat gtactggtca tattgaaaac cgtgataaga actgctctta cgactacctg
    22801 ctgcacccgg caatttctat ttctaaaatc attggcggtt gcggtcctac tgcacagtaa
    22861 gtctctgaca gaaatcggct aacaccggtt taattgcccg acgccctctg gattttccga
    22921 gggcgtattt gtttctatgt ccttcttgcc cccgcccgta aaaatatttt cactttaaat
    22981 tcaatttgat aactacatcc attagccaca gcgcgatcct gtgcgaaatt ttgtgatctt
    23041 cctccacatt acataacatc atatgttgtt atattcatca tgcattgtca tgttaccttt
    23101 taaatgactg caaactctcc cctacaacgt attggacaag aaaaaggtat cgctatggga
    23161 agccaggaac tccaacgcaa gctcggattt tgggccgttc ttgcaatcgc cgtcgggaca
    23221 accgtcggct ccggtatttt tgtatctgtg ggtgaagtgg caaaagcagc gggcacgccg
    23281 tggcttacgg tgctcgcgtt tgtcattggc gggttaattg tgatcccgca aatgtgcgtc
    23341 tatgcggaac tatccaccgc ttatccggaa aatggcgcag attatgttta tctgaaaaat
    23401 gccggaagcc gaccgctggc tttcctctcc ggctgggcca gcttctgggc caacgatgcg
    23461 ccgtcattgt cgattatggc gctggcgatt gtcagcaatc ttggcttttt aacgcctatc
    23521 gatccgttgc tcggtaaatt tatcgccgcc ggattaatta tcgcctttat gttgctacac
    23581 ctgcgctccg ttgaaggcgg cgcagcgttt cagacgctaa ttaccatcgc caaaattatc
    23641 ccgttcacta tcgtcattgg ccttgggatc ttctggttta aagcggagaa ttttgccgcc
    23701 cctaccacca ctgcgattgg cgcaacgggc agctttatgg cgctgctggc ggggatctct
    23761 gccaccagtt ggtcgtatac cggcatggcc tctatctgtt atatgaccgg cgaaattaaa
    23821 aaccccggaa aaaccatgcc acgagcgctg attggttcct gtctgctggt tctggtgctc
    23881 tacaccctgc tggcgctggt gatttccggc ctgatgccct tcgacaaact cgccaattct
    23941 gaaacgccga tttccgacgc cctgacctgg atccccgcac tcggcagcac cgctgggatc
    24001 tttgttgcca tcacggcgat gatcgtcatt cttggttcgc tttccagctg cgtgatgtac
    24061 cagccgcggc tggaatacgc gatggcgaaa gacaacctgt tctttaaatg cttcggccat
    24121 gtgcatccga aatacaacac gccggatgtc tccatcatcc tgcaaggggc gctggggatc
    24181 ttcttcatct tcgtttccga tctcaccagc ctgctgggtt atttcaccct ggtgatgtgt
    24241 ttcaaaaata ccctcacctt cggctccatc atctggtgtc gtaaacgcga cgattacaaa
    24301 ccgctgtggc gtactccggc tttcgggctg atgaccaccc tcgccattgc gtcaagcctc
    24361 attctggtcg cctcaacctt tgtctgggca ccgattcccg gccttatctg cgccgtcatc
    24421 gttattgcta ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg ccagctcaac
    24481 gctttgtcgt aatgttatct ggagaaaata aaatgttgga tattgataaa agcaccgtgg
    24541 actttctggt caccgaaaat atggttcagg aagtggaaaa agttctcagc catgacgttc
    24601 cgctggtgca cgccatcgtg gaagagatgg tgaagcgcga cattgatcgt atttatttcg
    24661 ttgcctgcgg ctcgccactc aacgcggcgc aaacggcgaa acatctggcg gatcgctttt
    24721 ccgatcttca ggtctacgcc atttccggct gggagttctg cgataacacc ccgtatcgcc
    24781 tcgacgatcg ttgcgcggtg attggcgttt ctgactacgg taaaacggaa gaggtaatca
    24841 aagcgctgga gctgggccgg gcctgcggcg cactcactgc ggcgttcacc aaacgcgcgg
    24901 acagcccgat tacctcggcg gcggaattta gcattgatta tcaggccgac tgtatctggg
    24961 aaattcacct gctgctctgc tacagcgtgg tgctggagat gatcacccgc ctcgcgccga
    25021 acgcggaaat cggcaagatc aaaaacgatc tcaagcagtt gccgaatgcg ctcggtcatc
    25081 tggtacgcac ctgggaagaa aaaggccgcc agcttggtga actggccagc cagtggccga
    25141 tgatttatac cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa ggcattgtaa
    25201 cgctgatgga atttacctgg acgcacggct gcgtgattga gagcggagag ttccgccatg
    25261 gcccgctgga gattgtcgaa ccgggcgttc cgttcctgtt cctgctcggc aatgatgaaa
    25321 gtcgccacac caccgaacgc gccattaact ttgttaaaca gcgtactgac aacgtgatcg
    25381 tcatcgatta cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg ttcctgatgt
    25441 tcgtgccaat ggagtggctc tgctactacc tgtctattta caaagatcac aacccggatg
    25501 aacgccgcta ttacggtggt ctggtggaat attaatccct ctcccggccc ggcaacgggc
    25561 cggatttacg caaggagtta cccgatgaaa acaggtatgt ttacctgcgg ccaccagcgg
    25621 ctgcctattg aacacgcatt tcgtgatgca agcgagctgg gttacgacgg catcgaaatt
    25681 tggggcggtc gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat caaacaaatc
    25741 aaggcgctgg cgcagacgta tcagatgccg attatcggct atacgccaga aaccaacggc
    25801 tatccgtata acatgatgct gggcgatgaa catatgcgtc gcgaaagcct cgacatgatc
    25861 aagctggcga tggatatggc aaaagagatg aacgcgggtt atacgctgat ttccgcggcc
    25921 catgcgggct atctcacgcc acctaatgtt atctggggac ggctggcaga gaacctaagc
    25981 gaactgtgtg agtacgcgga aaacatcggc atggatctaa tcctcgaacc gttaacgccg
    26041 tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc gctggtgcct
    26101 tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc ggaaccggtg
    26161 atgagttatt tcgacaaact gggcgataaa ttacgtcatc tgcatattgt cgacagcgac
    26221 ggggccagcg acacgcatta cattcctgga gaaggcaaaa tgccgctgcg ggaactgatg
    26281 cgcgatatta ttgagcgggg ctatgagggt tactgtacgg tggagctggt gacgatgtat
    26341 atgaacgagc ccagactcta tgcccgccag gcgctggaac gctttcgcgc gctgctgccg
    26401 gaggatgaga gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat atctacccgc
    26461 aactgaataa agcgttttct ggcggtaatg cggtcaatgt ggcggtgtac tgcactcgct
    26521 acggcataca gccgggatgc attacctggg tgggtgacga tgactacggc acaaagctga
    26581 agcaggatct cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa cacggcgtta
    26641 ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac tacaccgaag
    26701 gcgtgatggc cgactttgcc ctgagtgaag aggattacgc ctggctggcg cagtatgaca
    26761 ttgtgcacgc cgcaatctgg ggacatgcgg aagacgcatt cccacagctg cacgctgcgg
    26821 gcaaacttac cgctttcgac ttctccgaca agtgggacag cccgctctgg cagacactgg
    26881 tgccgcatct cgattttgcc tttgcctccg caccgcaaga agacgaaacg ctgcgtctga
    26941 agatgaaagc gattgttgcc cgtggcgcag gaacagtgat tgtcacgctg ggtgaaaacg
    27001 gcagcattgc ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg gtgacggtta
    27061 tcgacaccat gggtgccgga gattcgttca ttgccggatt cctttgcggc tggtctgcgg
    27121 ggatgacatt accgcaggcg atagcgcagg gaacggcgtg cgcggcgaaa accattcagt
    27181 accacggtgc ctggtaggta taacgttggc gtgagcatct tcacgccaac gtgctgttac
    27241 ttgccggaaa acgaccctat aatccgagta attcattctt tatttcaggg tcgattatgt
    27301 cagctacgga ccgctactct catcaactcc tctacgctac cgtccgccag cgactgctgg
    27361 atgatatcgc gcagggggtt taccaggccg ggcaacagat ccctaccgaa aacgagcttt
    27421 gtacacaata taacgtcagc cgcattacca ttcgcaaagc catcagcgac ttagtggcag
    27481 acggcgtact gatccgctgg cagggaaaag gcacctttgt acaaagccag aaagttgaaa
    27541 acgccctgct tactgtcagt ggttttaccg attttggcgt ctcacaaggc aaggcgacga
    27601 aagagaaagt gatcgaacag gaacgggtca gcgccgcgcc gttttgcgaa aagctgaaca
    27661 tccccggaaa cagcgaagtg ttccatctct gccgggtgat gtatctcgat aaagagccgc
    27721 tgtttattga tagttcatgg atcccgctgt cgcgttatcc tgactttgat gagatttacg
    27781 tcgaaggaag ctccacctat cagttatttc aggagcgttt tgacacgcga gtggtcagcg
    27841 acaaaaagac catcgatatc tttgccgcca cccgcccaca ggcaaaatgg ctgaaatgcg
    27901 aactgggcga accgttgttt cgcatcagca aaatcgcctt tgatcagaat gacaaaccgg
    27961 tgcacgtctc cgaactcttc tgccgcgcca atcgcatcac cttaactatt gataataaaa
    28021 gacatta
//
LOCUS       DACAPB010000042        28014 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.042, whole genome shotgun sequence.
ACCESSION   DACAPB010000042 DACAPB010000000
VERSION     DACAPB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.042"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..205
                     /locus_tag="IT904_001645"
     CDS             <1..205
                     /locus_tag="IT904_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191440.1"
                     /translation="SIASKVFHAFIDLVNKIRGKKDFMYICTLCCFAEEVKGDYSHYR
                     TFLFDIGNQYKVKLTQSGKKSSL"
     gene            complement(544..1737)
                     /gene="tyrB"
                     /locus_tag="IT904_001646"
     CDS             complement(544..1737)
                     /gene="tyrB"
                     /locus_tag="IT904_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAP3191441.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     DAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1990..3069)
                     /gene="alr"
                     /locus_tag="IT904_001647"
     CDS             complement(1990..3069)
                     /gene="alr"
                     /locus_tag="IT904_001647"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP3191442.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(3122..4537)
                     /locus_tag="IT904_001648"
     CDS             complement(3122..4537)
                     /locus_tag="IT904_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAP3191443.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4620..5603
                     /locus_tag="IT904_001649"
     CDS             4620..5603
                     /locus_tag="IT904_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAP3191444.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGTVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5769..6011)
                     /gene="pspG"
                     /locus_tag="IT904_001650"
     CDS             complement(5769..6011)
                     /gene="pspG"
                     /locus_tag="IT904_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAP3191445.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(6145..7182)
                     /gene="dusA"
                     /locus_tag="IT904_001651"
     CDS             complement(6145..7182)
                     /gene="dusA"
                     /locus_tag="IT904_001651"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAP3191446.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDIDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7545..8252)
                     /locus_tag="IT904_001652"
     CDS             complement(7545..8252)
                     /locus_tag="IT904_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAP3191447.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVETKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(8276..8530)
                     /locus_tag="IT904_001653"
     CDS             complement(8276..8530)
                     /locus_tag="IT904_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191448.1"
                     /translation="MLKIIPGATRYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8848..9363
                     /gene="zur"
                     /locus_tag="IT904_001654"
     CDS             8848..9363
                     /gene="zur"
                     /locus_tag="IT904_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAP3191449.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(9405..9614)
                     /locus_tag="IT904_001655"
     CDS             complement(9405..9614)
                     /locus_tag="IT904_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP3191450.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(9730..11109)
                     /gene="dinF"
                     /locus_tag="IT904_001656"
     CDS             complement(9730..11109)
                     /gene="dinF"
                     /locus_tag="IT904_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAP3191451.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(11128..11736)
                     /gene="lexA"
                     /locus_tag="IT904_001657"
     CDS             complement(11128..11736)
                     /gene="lexA"
                     /locus_tag="IT904_001657"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAP3191452.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(11846..12214)
                     /locus_tag="IT904_001658"
     CDS             complement(11846..12214)
                     /locus_tag="IT904_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP3191453.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            12385..14808
                     /gene="plsB"
                     /locus_tag="IT904_001659"
     CDS             12385..14808
                     /gene="plsB"
                     /locus_tag="IT904_001659"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAP3191454.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(14963..15835)
                     /gene="ubiA"
                     /locus_tag="IT904_001660"
     CDS             complement(14963..15835)
                     /gene="ubiA"
                     /locus_tag="IT904_001660"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAP3191455.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(15848..16345)
                     /gene="ubiC"
                     /locus_tag="IT904_001661"
     CDS             complement(15848..16345)
                     /gene="ubiC"
                     /locus_tag="IT904_001661"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAP3191456.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(16568..18148)
                     /locus_tag="IT904_001662"
                     /pseudo
     CDS             complement(16568..18148)
                     /locus_tag="IT904_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(18377..19297)
                     /gene="malM"
                     /locus_tag="IT904_001663"
     CDS             complement(18377..19297)
                     /gene="malM"
                     /locus_tag="IT904_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAP3191457.1"
                     /translation="MKMNKSLIVLCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(19540..20880)
                     /gene="lamB"
                     /locus_tag="IT904_001664"
     CDS             complement(19540..20880)
                     /gene="lamB"
                     /locus_tag="IT904_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAP3191458.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADNNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(20952..22067)
                     /gene="malK"
                     /locus_tag="IT904_001665"
     CDS             complement(20952..22067)
                     /gene="malK"
                     /locus_tag="IT904_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAP3191459.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            22432..23622
                     /gene="malE"
                     /locus_tag="IT904_001666"
     CDS             22432..23622
                     /gene="malE"
                     /locus_tag="IT904_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAP3191460.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            23776..25320
                     /gene="malF"
                     /locus_tag="IT904_001667"
     CDS             23776..25320
                     /gene="malF"
                     /locus_tag="IT904_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAP3191461.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            25335..26225
                     /gene="malG"
                     /locus_tag="IT904_001668"
     CDS             25335..26225
                     /gene="malG"
                     /locus_tag="IT904_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAP3191462.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            26597..>28014
                     /gene="xylE"
                     /locus_tag="IT904_001669"
     CDS             26597..>28014
                     /gene="xylE"
                     /locus_tag="IT904_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAP3191463.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLE"
BASE COUNT         7106 a         7170 c         6726 g         7012 t
ORIGIN      
        1 ttctattgca agtaaagttt ttcacgcatt tattgattta gttaacaaaa tcaggggtaa
       61 gaaagacttt atgtatattt gcacgctttg ctgttttgcc gaggaggtta aaggtgatta
      121 ttctcattac aggacctttt tatttgatat tggtaatcaa tataaggtta aacttacaca
      181 aagcggaaaa aagagttctc tttaacatta gaatttaacg atactataat tgaatctcag
      241 aaagtcacag gcaataaggc aaagcatatt cttgaggata tagaaatatt ctatcgtaac
      301 aaacccgata cttattatta aatttaataa tggttagatg tgcagaaatt attactccac
      361 ccaaaaaaaa caggataagg aattccagat aattgggata aacttaacaa taaaagaact
      421 aacaacgttt attatctctc acgtagaacg atggcatcaa aaaaatcatc tataaagaaa
      481 gagtgaaagc gacgacagtt taatttcact gcaggctggg tagctccagc ctgctttcct
      541 gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg tatttaaccc
      601 ggcgacacac atgcgaccgc tggcgatgag atagacacca aattcttcac gtagtcggtc
      661 aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa gcagataatc
      721 gaaattgcgt tctggcatct ctgtgcttaa taccttcacc aattcctgac gcattgccag
      781 aatgcgagta cgcatctctt ctacttccgc cagccagctg gctttcaatg cctcgtcatt
      841 cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac
      901 tgttgctttc aattgcccca gtacgcggcc agcggcttcg gcatcttcac acataacaga
      961 aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat tgctcaccag
     1021 agcgggtaat ccagcgctgg caatggcgcg aatagcgtag gcatcctctt ccataccggc
     1081 atcaaatcct tgataggcaa tatcgaggaa tggaataagc tcgcgggctt tgagaatttc
     1141 aatcaccgca tcccactgat cattagtgag atcggcaccc gttgggttgt ggcaacatgg
     1201 atgcagcaac acaatactgc gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa
     1261 gcgcacgccg ttagtcgctt cgtcatacca ggggtaagta ctcacttcga atccagcccc
     1321 ggcgaatatt gctacgtggt tttcccaggt aggatcgctg acccagacgc ctgattccgg
     1381 gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc ccggagccgc caagggtttg
     1441 aatggttgct acgcgctgtt gtttcagtac cggatggtcc gcaccaaaca gcagcggcgc
     1501 aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag cgccatgagg
     1561 ctgcgcattc aggcgcgctt ccgcctccgc cacggcttgc agttgtggaa taattccgtc
     1621 ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt cttctttaaa
     1681 acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg
     1741 atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga
     1801 tcgagtggat gttcaggagc gaacggcaat tagcaacaga gtgagactca tgacaaacgt
     1861 acatccgcca gaggcacgac ctttataaga gcatgaaaga acatcaactt attgaatttt
     1921 taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca atcaaatatt
     1981 acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg taataagttc
     2041 gtaagcgctt actttcgtca tttcagcgat acgttctacg ggcaaacctt cgccccataa
     2101 aatgaccgga tccccggctt tgtcctgcgc ctgtggacct aagtctacgc agatcatatc
     2161 catcgccacg cgcccgacaa tcggtacttc gcgaccgttc accagcactg gcgtaccgga
     2221 cggcgcggcg cgcggataac catcgccata gcccatcgcg actacgccaa gacgggtatc
     2281 acgttcgctt acccaggttc caccataacc aacaggctct ccggctttat gctcacgcac
     2341 ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg caccggtgga
     2401 gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc agtcaaaatg
     2461 cgactgtggc cacagcagaa tgccacccga cgcggcaatg gaacgttgac caggtttgcc
     2521 ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt ttggttcatc
     2581 cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc actgggtcag
     2641 gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca taccggtatc
     2701 gagtttcatc cagacggtaa ccggctcgtc caggctagcc tcttccagcg cagccagctg
     2761 ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc
     2821 atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac gcagtcgcag
     2881 agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc
     2941 aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac tggcaggggc
     3001 cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca caacagttgc
     3061 cgcttgcatt tgtgttcctt gataagtgtt tgctttaatt acctaattca taaaataatt
     3121 attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa
     3181 aggtcaggcg taccgtcccg attgggccgt tacgttgttt accgataata atttccgcga
     3241 tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac atgatcaagt
     3301 ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccggg cgtttgtcgg
     3361 cacgttgttc cagagaacgg ttcaactggg acagcgccac caccggcacg ttcagttctt
     3421 tcgccagtgc tttcagcgag cgagagattt ctgcaatttc cagcgtacgg ttatcggaaa
     3481 gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt
     3541 cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg gaggagtcat
     3601 cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt
     3661 cttcgtcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac agcgacgcca
     3721 gagaacgcat catgatctgt tctgatggca tctccagcga gaagataagt accggtttat
     3781 cctgcaacat cgccgcgttt tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg
     3841 gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt ttgttgagat
     3901 cgtcataacc ggtgtttacc ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa
     3961 tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg tctttgttcg
     4021 cacgactttc ggcaatttta aagacgcggg attcagccag atccagcaga tcttcgctgg
     4081 tacgcccctg cggatcaaaa ccagcttcgg caatctcatt cgcaaccgag atcatctcac
     4141 ggacaacggc acgttcacgc acgatgtccg cataggcact gatgttagcc gcacttggcg
     4201 tattttttga cagctctgcc agataagcaa aaccaccgac gctatcgagt tgcccctggc
     4261 gttccagcga ttccgcaaga gtaatcagat cgatagggct accgctttcc tgcaaacgcg
     4321 ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa atcgtctgct accacacgct
     4381 cggctacatc atcccagcgt tcgttatcta gcattaaacc gcccaacacc gactgctccg
     4441 cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt tcgcggggtt
     4501 cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt accgagataa
     4561 agaatgggtc gaaactttac catatgaagc agaccctgac gatacgttct ggaggacaca
     4621 tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa gccgtagagt
     4681 tcactcctgc cgatccggcg gagaatgaaa tccaggtcga aaataaagcc atcggcatca
     4741 attttatcga cacatatatc cgcagcggcc tttacccgcc gccatcgcta cccagcggat
     4801 taggcaccga agcagcaggc atcgtgagta aagtcggcag tggtgtaaag catattaagg
     4861 caggcgatcg tgtagtctat gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca
     4921 ttattgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa gctgcggcat
     4981 ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa aacctatgaa attaaacccg
     5041 atgagcagtt cctgttccac gcagcagctg gcggcgttgg cttgattgcc tgccagtggg
     5101 caaaagccct gggcgcgaaa cttatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg
     5161 cgctaaaagc gggcgcgtgg caggttatta actatcgtga agagaatctg gtcgagcggt
     5221 taaaagagat caccggcggt aagaaagtgc gcgtggtgta cgattccgtg ggcagagaca
     5281 cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtgagt tttggcaact
     5341 catcaggtac ggttaccggt gtgaacttag gcattctcaa tcagaaaggc tcgttgtatg
     5401 tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc gaggccagta
     5461 atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga
     5521 aatatccgct gaaggatgcg cagcgtgcgc atgaaattct ggaaagccgg gcaacgcaag
     5581 gttccagcct gttgattcca taaaagaaat agggcttcca cctgggaagc cctttctttt
     5641 tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc aatagtgaca
     5701 gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt gatcatccgc
     5761 acaatccctt agtaacgcca gcggtcataa cgctgatatt tcggcacttt tggtgcttta
     5821 atcgccttaa taacccacac caccgcaatc gccagtagta accacggcag caacttaatc
     5881 atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag cgcggcgata
     5941 atgcccagca acgaaacgcc ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt
     6001 agttccagca tgatgcactc ccaaatatga aatctcttgc tggcattaca agaatcatgc
     6061 caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga
     6121 atttgactat tttttggtga aaagttaacg cttatccgcc accagtttga gcgcgtgttc
     6181 cagcacatta atgtctgcac ccgctttatg ggcattttca cttaaataac gccgccactg
     6241 ccgcgcgcca ggaataccct ggaacaagcc caacatatgc cgggtaatat gaccgagata
     6301 cgtcccctgg ctgagttcac gctcaatgta cggatacatg gcgcgcacta ccgccaccgg
     6361 atcggcatcg atatccgagg aaccaaagat ctcccggtct accgccgcca gaatacccgg
     6421 attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaggt gtgctttggc
     6481 ctcttccagc gacttgatac caccgttaat cgacattgtc agatgcggaa agtcacgctt
     6541 cagttgatac acacgcggat aatcgagcgg cgggatctca cggttttctt tcggacttaa
     6601 ccccgaaagc caggctttac gtgcatggat gatgaacatc tcacactcgc ctttgccgga
     6661 aacggtgttg atgaaatcgc agagaaattc atagctgtcc tggtcgtcga tgccaatacg
     6721 cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc gctttcacgc agtcggcaac
     6781 cagctgcgca ttacccatca gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg
     6841 gcagccgaca ttcaggttga tctcatcata tccacgcgct tctgccagct tcgcacactg
     6901 cgccagcgcc gccggatcgc taccgccgag ttgcaacgct accggatgtt cttcttcact
     6961 atacgccagg taatcacctt taccgtgaat aatcgcccct gtagttacca tttcagtata
     7021 cagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc
     7081 gagcattggt gcaacgctaa accgaccact ccagtgaacg tcagtttttt caggcattgc
     7141 gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat gtagagatta
     7201 ttttatcgat atatcatggg gttatgttat tcagcatcac tgttcaggag gctcaatagc
     7261 ggggtactat accataacaa caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc
     7321 tctcaaggca tgaagctctc acgaagtgat ggaaataatc ttattagccg ttaactttgt
     7381 taaggccaat gataaacaat ccaggttcga cgataaataa aaaatcacac attaaactct
     7441 ggtgatatat ctccctgcta atagcattga tagagaaaaa agaacccaat aaatattggg
     7501 tccttttata taatgccttc catactatcg aagaacttca catattattt ctccgattta
     7561 accccgaaca aatcataaat taatttagaa gtgtctgtaa gtattttaat ctcttccttt
     7621 gaggttgggt caaacgactt cgcgaagcta attaattgtg gtgcagcatc tcgcattttg
     7681 cttaaaatat caggatcgag cgttccttca ttcaccagat gtgacatctt atccagatag
     7741 ccatcaaaat tcatttctcc cccatcagcc agacgcttca tctctcccag gtacttcttc
     7801 atatcacgtt gagataattt ttcaaactga gctttcaggt agctctcatt attttcatta
     7861 atatacactg tttctgataa atctgcgtag gcactagaat atgaaagtga aattaacagc
     7921 gaagcaaata attttaagct gttgtcatca tgttcctggc aggcattaac aaacgttaaa
     7981 aaaccagaac ctattgcttg aaaatgtata ttcgctaaag gttcattatc cttagattcc
     8041 tcataaaata gctgaaccgt ggagggaagg tcattcttag tttctacatc aaaagtacat
     8101 tgttcaatgg gcattgcatc tttattatcc ccaagattac atatactatt aatcgcgaaa
     8161 tgaaaaatag cccggtcaac aactgatgca ataaagtcac gatcgtttaa atcctcatca
     8221 gtgcaatcgt caatatactt attgacccac attttgagat catttatttg ctcaattaaa
     8281 aacacatttt tttacgtgca tgccataaat tatgcgtgat tcgttgaata aacaaatcta
     8341 tttttgcaat aattatatcc cgatgacgca gcgctgcata atcaatagat ttaccatata
     8401 tattatttaa tttacctttg attgaacctc tattatctaa tttatctttg attgcatctt
     8461 cattatcgaa ttgatttgaa ttgagtgttt tattaaagta tctagttgca ccgggtatta
     8521 tcttcaacat gattcatcta ccttttttgt cgtaccgtgt cctccttata attcttttgc
     8581 catctggtca ctatcaaaaa tcgctcataa tttctgaact acaggtatgc gtttaaaccc
     8641 agttaaataa atagcgagtt aacttgaagc aatttattta aaaatgaatt attaattatt
     8701 atcgtgttga taatggtacg acaaggcaac acaacacctt acctacaaaa cagaaaatca
     8761 acattacggc aacaataagg gttctcggtg tttttcctgg aacatggtaa agtaaggaca
     8821 ttcttaaccc ccactttgag gtgcccgatg gaaaagacca caacgcagga gttattagcg
     8881 caggctgaaa aaatctgcgc gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg
     8941 ttgcgcctga tgagtctgca ggatggcgct atcagcgctt atgatctgct tgatttactg
     9001 cgcgaagctg aaccgcaagc caagccgcca acggtttatc gcgcgctgga ttttctgctt
     9061 gagcaaggtt ttgtgcataa ggtggaatcc accaacagtt atgtgctctg tcatctgttc
     9121 gaccagccca cccatacgtc agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa
     9181 gagtgtgctg aaggcgtgga agacatcatg catacgctgg cggcaaaaat gggttttgcc
     9241 ctgcggcata atgtgattga agcacatggg ctctgtgcgg catgcgtgga agtggaagcg
     9301 tgtcgtcatc ctgaacagtg ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt
     9361 taagagggcg tacatccttg tacacgtcgg gcaggaggga ttaattacca gcgatattca
     9421 ttgcgggttt cccaatccac gacctctttt tctgcctgat ctttctgata accataacgt
     9481 tcctggattt taccgaccag ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg
     9541 gtcagtttgc cccattgctc tttcacttta cctttaaact gtttccagtt accgccggct
     9601 tcatctttat tcataatcaa gacctcatcg ttaggttgtg aatgagagta cgttcacttt
     9661 tcttctgaac gtgagattaa gtgtagtcag caatttggct taggattatt tattcagaat
     9721 ttttaaccgt cacgttgcgg caaaccaggt accgttgcgc cagtgacgcc gccagatagc
     9781 cgccagagaa agcccgcgca acgccagaaa gacggttaat gccagccaca aaccatgatt
     9841 acccagccac ggcagcgtaa ggagcgtcag cgcaaaacct gcggcggcca ccgccatact
     9901 gttacgcatt tcggcggcac gcgttgcgcc tataaacatg ccgtccagca gataacacca
     9961 gactccaacc aacggcaaaa tcacctgcca gataagatag cggtcggcca gctcctgaat
    10021 ctgggttaac gacgtcagta acgcaatgat gtgttcccct gccagcaaat aaaccaccga
    10081 aaacagtagc gctacgatcc ccgactggcg gcacgctgcc cgccagacat ccagcaactg
    10141 gctaccgtcg cgcgcaccgt atgcctgacc ggaatgtgct tcaaccgcgt aggcaaaacc
    10201 atccagcgca taggcggtaa aggtgagtag cgtcatcaga accgcgttaa cagcgataat
    10261 gtcactcccc agtcgcgcac caagtacggt gatcgcgccg aaacagagtt gcaacaacag
    10321 cgaacgcagc atgatatcgc ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc
    10381 agttttcagc atttcgccgg aaattccgcg tagtttgagg attttacgca ccattagcag
    10441 accaatcagc aatgttgcat attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt
    10501 catatgcagc cccatcacca gccagacatc cagcacaatg ttgaggatat tgccgaccac
    10561 taacaaaatt actggcgcac gggcatattg cacgccgagt aaccaaccga gtaataccag
    10621 attcgccagc gacgccggag cgcttaacca gcggatttca agaaagcgcc gcgcctgttc
    10681 caggaccgct tcactaccgc caacaatatg cagcgccaga tcgataatcg gtgtacgcag
    10741 cagcgcaatt aacgccccag cccccaacgc caacagcaac ggttgcacca gcgcacgggc
    10801 taatgcctga ggatttttgg caccataagc ctgcgcagtc agcccggtgg tgctcatgcg
    10861 taaaaacagc aacagcataa agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc
    10921 caaataaacc gggctatcaa gatgaccaat taccgccgta tcgaccagtc ccagcaacgg
    10981 aacggtgata ttggagaaaa tcatgggtaa ggcgagatgc cagagtgctt tatcagatga
    11041 agtgaggaat gccatgcaga caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc
    11101 tggcggcatc gcggtctcag agatatgtta cagccagtcg ccgttgcgaa taaccccaac
    11161 cgccagccct tcaatggtga agctctgctg acgaaggtca acgacaattg gtttaaactc
    11221 gctattttct ggcaacagtt cgactttatt gccctgtttt ttcaggcgct taacggtaac
    11281 ttcgtcatca atacgtgcga caacgacctg accgttacgt acatcctgag ttttatgcac
    11341 tgccagcaag tcgccatcca taatgccgat atctttcatc gacatcccgc tgacgcgcag
    11401 caggaaatca gcattcggct tgaacaagga aggatcgacc tgataatgac cttcaatatg
    11461 ctgttgcgcc agaagcggtt caccggcagc cacacgacct accagcggca acccttcttc
    11521 ctcttcctgc aacagacgaa tcccgcgtga tgcgccggaa acaatttcaa taacgccttt
    11581 gcgtgccagc gccttcagat gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg
    11641 cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg atgtgatcac ggatgagatc
    11701 aaacacctct tgttgcctgg ccgttaacgc tttcattccg ccccctgggt gtatatacag
    11761 ttatgctgtg agtatataca gcaaaaggcg attttggaac cataaactgc acaataaacc
    11821 agagatttat cgaaatctgg aagggttatc caaaatgcga ccataacagg atgcaccagg
    11881 ttatcacggc gacgataatc gcaatcagca ccgcggcgga tcccatatct ttagcgcgtc
    11941 cggaaagctc atggtattca gagccaattc ggtcaaccac tgcttcgatg gcgctattga
    12001 ggatttccac aatcatcacc agcatcacgg agctgataag cagcacgcgg gtaattgcgt
    12061 ccacatccag ccagcaggcg atgaccaccg ccaacaatac cgctacgcct tcctgacgga
    12121 atgccgcttc gttgatccat gcagcgcgta aacctttcca ggaatagcca gcagctttga
    12181 taattcgggt gaatccagtg gtattattgg ccattgaaag aaccttttta cattatgagc
    12241 gtcaatatca gtgtaccgtt aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca
    12301 tgagcggata ccacagaatt tcccatgact ttctgctatc cttgccgcgc atttgcatta
    12361 ttaaccagag gctttacatc gtttatgtcc ggctggccac gaatttacta caaattactg
    12421 aatttaccat taagcatcct ggtaaaaagc aagtctattc cggcagatcc tgccccggaa
    12481 ctggggctgg atacctctcg tccaattatg tacgttttac cgtacaactc gaaagcagat
    12541 ttgctgacgt tacgcgccca gtgtctggct catgacttgc ctgacccgtt agagccgctg
    12601 gaaatcgacg gcacgctact gccgcgctat gtgttcattc acggcgggcc gcgtgtattc
    12661 acctattaca cgccgaaaga agagtctatt aagctgttcc acgactatct cgatttgcac
    12721 cgtagcaacc caaatctgga tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg
    12781 ccggggcgtg aaaaaggcga agtaaacccg ccgctgcgta tgcttaacgg cgtacagaaa
    12841 tttttcgccg tactgtggct cggtcgcgac agttttgtgc gtttctcgcc gtcagtttcg
    12901 ctgcgccgta tggcggatga acacggcacg gataaaacta tcgcacagaa actggcgcgc
    12961 gtggcgcgta tgcactttgc ccgtcaacgt ctggctgctg tgggcccacg tctgcctgct
    13021 cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat
    13081 gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg
    13141 gaagagattg cggcgaattt ctcttacgag atgattcgcc tgactgaccg tattctgggc
    13201 ttcacctgga accgccttta ccagggcatc aacgtccata acgccgagcg cgttcgccag
    13261 ctggcccacg acggtcatga gctggtatat gtgccttgcc accgcagcca catggactac
    13321 ctgctgcttt cttacgtgct gtatcaccag gggctggtgc cgccgcatat cgccgctggg
    13381 atcaacctga acttctggcc ggccgggccg attttccgcc gtctgggggc gttctttatt
    13441 cgccgtactt ttaaaggcaa taaactttat tccaccgttt tccgtgagta tctcggcgaa
    13501 ctgttcagcc gtggttattc cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg
    13561 cgtttgctgg atccgaaaac cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc
    13621 ggcacgcgtc cgattacgct gattccgatc tacatcggtt atgagcacgt catggaagtg
    13681 ggtacttacg ccaaagaact gcgcggcgcg acgaaagaga aagagagcct gccgcagatg
    13741 ctgcgcggtt taagcaagct gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca
    13801 atgccgttga tgacctacct taaccagcac gtaccagact ggcgtgaatc tatcgatccc
    13861 atcgaagcgg tgcgtccggc atggttaacg ccgacggtca ataatattgc tgccgatctg
    13921 atggtacgca ttaacaacgc aggcgcggca aacgccatga acctgtgctg tactgcgctt
    13981 ctggcgtcac gtcagcgctc actcacccgc gagcagttaa ccgagcaact caactgctac
    14041 ctggatctga tgcgcaacgt accttactcc acggactcta ccgttccttc agccagcgcc
    14101 agcgagctta tcgatcacgc gctgcaaatg aacaagtttg aagtcgagaa agacacaatc
    14161 ggcgacatca tcattctgcc gcgcgagcaa gcggtgctga tgacctacta tcgcaacaac
    14221 attgcgcata tgttggtgct gccttcgctg atggcggcaa tcgtcaccca gcatcgccac
    14281 atctcccgcg acgtattgat ggagcacgtc aatgtgcttt acccaatgct gaaagcggag
    14341 ctgttcctgc gctgggatcg cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag
    14401 atgcaacgtc aggggctgat taccctgcaa gatgatgagt tgcatatcaa cccggcgcat
    14461 tctcgcacgc tacagctgct ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc
    14521 accttctggt tgttgagtgc caacccgtcg atcaaccgcg gtacgctgga gaaagagagc
    14581 cgcaccgtcg cgcaacgtct ctccgtgctg cacggcatca acgcgccgga gttcttcgac
    14641 aaggcggtgt tcagttctct ggtgctgaca ctgcgtgatg aagggtatat cagcgatagc
    14701 ggcgatgccg aaccggcaga aacgatgaag gtttatcagt tgctggcgga gttgattaca
    14761 tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg aagggtaatc agagagaatt
    14821 gccggatgcg gcgaaaacgc cttatccggc cttccataat ctgcaaattc aataaattgc
    14881 gatgtgttgt gtaggcctga gaagcgccgc gcatcaggca acccagaaga aagccggatg
    14941 atcatccggc ttttttacat catcagaaat gccagtaact cattgccagc cctaaaaata
    15001 gtaccagacc aacatagtta ttattcataa atgctttaaa gcaggcttca cgctcgcggt
    15061 tggcaatcag tttttgttga taaacaaaca gcgcgccagc caccagaatt gaccagtaat
    15121 atccccagcc taagccattt aactcaccga tgatcgccat cagtgccagt acgccaatct
    15181 gcaaaatacc aataatcaat ttatcgtatt ggccgaacag gattgccgtg gatttaatgc
    15241 caatcttcac atcatcatcg cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc
    15301 agagaatatt ggcgaggaac attaaccagc aactcaatgg caccgactca ctcacagcgg
    15361 caaaagccat tggaatcgac cagccaaacg ccgcgcccag caccacttgc ggtagatggg
    15421 tataccgctt cataaacggg tacacccacg ccagcgctag cgcggcaatc gacaacagaa
    15481 tggtcatcgt attcagcgtc agcaccagta aaaacgaaat cagtaccagc acgacaaaca
    15541 gcgcgcgcgc ctctttctct gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct
    15601 taacatgacc atcaaacttg cggtcagcat aatcattcac cacacatccg gcagcgcgca
    15661 tcagccagac acccgcgaca aacaccgcca ggatccagag ctggggaacg cccggtgtcg
    15721 ccacccacaa cgcccataat gttggccaga gcagcagtaa cgcgccaatt ggcttatccg
    15781 tacgcattaa gcgatgaaac gccagcagct tattctgcgt cagactccac tccatatttt
    15841 tttcctctta gtacaacggt gacgccggta aaaacagttc tgttagcaac agcggtttac
    15901 cgcttaatcg caggcgggaa cgtcgccccc acagcccggc atcacggcct atctcaataa
    15961 agtcccgggt taatgtcgat gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt
    16021 tttgtaacgc cagctccggc ccgcttaacg ttgacacagg aacgacggta cgaccggcaa
    16081 gccacggttc accatcggca cataacaaaa tttcacgtaa ccagtaacga gactctttcg
    16141 gcagcagcgg cagttcttcg gggatttcat tctgctcgac aaacccttcg cggatcatcg
    16201 tcacgcttac cgtttttccc tgctgttcaa aacgttttgt catggaatcc tccagcaaca
    16261 gccagtcgag cagttgcgga tccagggcag ggatctcttt aaaatagcgc agcgcacgca
    16321 gttgcgttaa cgcggggtgt gacatgccga actctccgtt acataacgta aaggcattgt
    16381 atcgcagaaa agtgtttacg ggagatgtaa agcaaattaa gtgatctaat acgcaacaaa
    16441 aacgcaacaa tcgcatgaag tggtaaaacg atgttgaacc ttacgcttta ccgatgcccg
    16501 tgctggcgag ggcaaccgta ggggcatcac cgtccaggat gccaggaacg gtgatgtttt
    16561 cctttactta aacaccctta ttcattatac gatgagctcg ccaactcgca gtaaaaattg
    16621 ggaaaagcgc atatttacca tgagatttgg agctgaaaag tgtttcctga gtaagattaa
    16681 caggcaacat ctcatgtctg gatttgtaga tatacgtgga attccaattg gtatcttttg
    16741 taggattcaa catatcatgt aagcgctggg agcttaccac cataactggc ccatcatcga
    16801 ctgaggagaa caggataaag atattatcgt ttcgtgttaa ttcattcgtg ttaaaggttc
    16861 ttaaattatc ttcagtttct ataaatatag gtgaaacatc atcactacga atccactctt
    16921 tataaagatg ccaaacattt gctttgatca tatcatccgt tgatcgtaag gcctgatcga
    16981 taagttggat aaaaaaattg ttatttttat caattagcca tttattcgta ttaaatctgt
    17041 taataaaatc gatcatctgt tgggtatcga aattcacctc actgaatcga tgttgacgca
    17101 taaaatcatc gaacttgtta tcttcatcag aatggatata actgcgtata atgggatctt
    17161 ttatataagg acaatggcta aatattttct tcatattctc aataaagttt ttattaacaa
    17221 tattaagtat ttttaattgc atcaccagtt ttacggccag aacggatttc aatatcattg
    17281 cattatctgg caatgtatca atcattttta taatgttatt gatataacca tcgagatctt
    17341 cattccaaaa tatatctgaa taatcaatag ttaattccaa ttgttctgta atgcaatctt
    17401 taaaattccc tgtcgacatt cgcacataat caaacataac agctgtcaaa ttcgatttaa
    17461 taaatgatat ttctttggca gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat
    17521 aacaattatc gaagtttgaa tttctaatct gacattcgcg aaaattacta tgaccaagtt
    17581 gactctgcga gaagtcaact gtatcaagaa tatttttgct gaaatcgaca cattcaccac
    17641 tcgctttgat aaaaatcaca ttgtgtaaac gtcgacatct aaatatgaca ttatcaacta
    17701 ttgcagtagt aaaattgcaa tccccaattt ctttacattc gaatgttaca tgttgtagat
    17761 ttgatgataa aaaagacacc ttcgatataa cgtcaccttt aaaatgacaa cttgttaaat
    17821 ccaaatcaga caggtcgata tacccctgcg catctttatc catcagaaaa ttgcaagcgc
    17881 attcaatttt tttcatttct gtagaatttt acacttatag tatcattact gattgagact
    17941 tcacgaaaca tatttattgc atgatttttt cgttccgttg ttttatattt gaaaaaatat
    18001 tgctgatata aataaagtta tctctgttta aacctggcac cccatttaaa ttacgtagta
    18061 aatcaagatc tactatatta tttccttgca ccacatcatg tttcatcaaa gagttattgc
    18121 cgccagaatt taggatatga cttatcataa aacctctgca taacagaaat aaaataacct
    18181 ggaaaagtag cctgaatgtc cagacactac cggcgaaagg aaaagaatct ctgataaggc
    18241 attgagataa tggatattct tagtgaagaa gaagtgagtt actataaaaa atcgctcata
    18301 tcccaggaag gaagcataaa aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca
    18361 gcactgtggg gcgtaattac cccttgcctt ttacactgct gataaaggtg gaacgggcag
    18421 atgtcgatcc caggcgttca gcttcatcaa gcagttttaa cgccttatca acatcacctt
    18481 tcgcgacagc gtttttgatc gcggtattaa aataactttc cgtgtcgttg agcatcggct
    18541 cgcttttctt caccggtgcc ggagcgggtg cagccacagc tggtgccgcc gtgttaccta
    18601 ccgtaaccgg agctggagcg gaagaaccaa ataaaggtcc taccaacacg ctggagctgg
    18661 agttcgtttt cactttcagt ttcagtaagc catcggtggt atgacgagca accggatcgg
    18721 ggatatccgg gatcgagtta ccgacgccct tggcataggc tttagccggg tcgagcagtt
    18781 gggtcgtctg ctggagatct ttttccgtgg taaagaccag aacataaagt ttttgctgcc
    18841 ccaacgccgg tgtcaggcgc ataacgcctt ccagccgatc tgcactcatc acgcctggtt
    18901 cctggtaggt gaaataactg ctggggaaga aggctgatgg ggtcatgttc tgatcaagaa
    18961 tcagcacgtt cggcgcaaaa acgctggttt gtttgttcac ttcgctggtc agcgtcaggg
    19021 tcagttcgcc aatgtttgcc gggacgctgt acgcagcaac cggaccactg atgccgggaa
    19081 cgttcagttg ttggccgccg gtcgccagtt gggtggtctg ggttttagat tgatcgaccg
    19141 gtgtccaggt gagttgttgc agcgcagcag atggaatggc tggcgcgtcg ctggtgtttt
    19201 gcggtacgta gttaacatcg gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg
    19261 ataaacagag gacgatgaga cttttattca ttttcattgt tttcacctca aaatctggag
    19321 ctcagcggta gccaggcaat agcgcgctaa acccgataat cagaggggct ttcgcccctt
    19381 tcagataatg acaacctgtt tttatgccgg atgcggcgta aacgccttat ccggcctaca
    19441 tttgacagcc gttgtaggcc tgataagacg cgcaagcgtc gcatcaggcg ttggttgccg
    19501 aatgcggcgt aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg
    19561 ggcaccgaag gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc
    19621 aggaacggct ttgccgaagt tcgcgttgtt atcagcgtta ccggtgtagt cgtaacccca
    19681 tttctcatcc cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct
    19741 gtcgccagcc tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt
    19801 gcgctgggat tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca
    19861 cttgtacatc gggcgaatac cgacggtcca ccacttggtg ccgttgtcgt tatcccagtt
    19921 gatatcctgg tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc
    19981 gtggtcgagg atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt
    20041 atcaaatgca acgccagaac cctgcgacag ccctttaccc tgcgaggtca tcgagtcagt
    20101 agcgtactga acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt
    20161 gaataaccag ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaagttggc
    20221 acgaccgtag tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa
    20281 acgcacatcg aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc
    20341 gaaagaggaa gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa
    20401 gccaacatcg atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc
    20461 gatcatatga acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggagcctgg
    20521 cagccattcg atcaggtttt taccctgcac gtttgcttca cggaaggccg gatcggtagc
    20581 ttcccagtca ttctgttgtg cgacggaata ggccacgtta gtgtcgaaat agaagctctt
    20641 atcgccctct ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt
    20701 gccaagacgg tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct
    20761 acctgtccaa ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc
    20821 agacattacg cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat
    20881 tctatctcct gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt
    20941 ttgtggggtg cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca
    21001 cggaacagat ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc
    21061 aacaccacgt cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatgg
    21121 atttgagttt cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca
    21181 gcgatatcac tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc
    21241 tggacatcac ggctttcaac tggcagccag acttgctgac gatttggcat cggcagctcc
    21301 acctgcactt gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc
    21361 gaaccgataa atccggcgac aaaacggtct gccggatagt ggtacagctc cagcggtttc
    21421 ccaacctgcg ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct
    21481 tcgacctgat cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag
    21541 atttcgatac gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc
    21601 aaaaatacgc ttggctcggc caccagcgta cggccaatcg ccacacgctg acgctgacca
    21661 ccggagagcg ctttcggttt gcgatccagc aaatgcgcca gttgtagcac ttccgccacc
    21721 tggttaacgc gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac
    21781 atgttttctg ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg
    21841 ccgcgttctg ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg
    21901 gtgatcgtct caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt
    21961 ccgacaaaca ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg
    22021 acctcgcccc aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt
    22081 tgtaacaacc tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga
    22141 tggcttaaat cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaaca
    22201 cggcttctgt gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat
    22261 cgtggcgatt ttatgtgcgc atctccacat taccgccaat tctgtaacag agatcacaca
    22321 aagcgacggt ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta
    22381 gagtccgttt aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata
    22441 aaaacaggtg cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg
    22501 gctctcgcca aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat
    22561 aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt
    22621 gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct
    22681 gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct
    22741 gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta
    22801 cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat
    22861 aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa
    22921 gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc
    22981 tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac
    23041 attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac
    23101 ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt
    23161 aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc
    23221 agcaaagtga attatggtgt aacggtactg ccgaccttca agggtcaacc atccaaaccg
    23281 ttcgttggcg tgctgagcgc aggtattaac gccgccagtc cgaacaaaga gctggcgaaa
    23341 gagttcctcg aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa
    23401 ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt
    23461 gccgccacca tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc
    23521 gctttctggt atgccgtgcg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc
    23581 gatgaagccc tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat
    23641 gcggcgtgaa cgccttgtcc ggcctacaaa accgaaacgt atgtaggcct gataagacgc
    23701 gtcagcgtcg catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga
    23761 atgaggaaga accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg
    23821 aaatggtcag tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac
    23881 gcacaagggg aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat
    23941 attttcgcca atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg
    24001 ggattattcg tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc
    24061 agcactaacc agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa
    24121 gcaggcaaaa cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc
    24181 agcgacggcg aaaccggcaa aaattacctc tccgacgctt ttaaatttgg cggcgagcaa
    24241 aaactgcaac tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg
    24301 attacccaga atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa
    24361 gtgatgatga gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt
    24421 gacggcacgt tgacgaataa tcagagcggc gtgaaatatc gtccgaataa ccaaattggc
    24481 ttttaccagt ccattaccgc cgacggcaac tggggtgatg aaaagctaag ccccggttac
    24541 accgtgacca ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa
    24601 ccgttcctcg ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg
    24661 gtggcggtcg gcatggttct ggcgtgtctg gtgcagtggg aagcgttgcg cggcaaagcg
    24721 gtctatcgcg tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt
    24781 ttcaaagggt tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt
    24841 ggcgtgaagc ccgcctggtt cagcgatccg accaccgccc gcacgatgct aattatcgtc
    24901 aatacctggc tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt
    24961 ccggacgatt tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt
    25021 aagattacgc tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc
    25081 tttaacttta acaacttcgt gctgattcaa ctgttaacca acggcggccc ggatcgtctt
    25141 ggcacgacca cgccagccgg ttataccgac ctgcttgtta actacaccta ccgcatcgct
    25201 tttgaaggcg gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc
    25261 ctgctggtgg gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa
    25321 gggagataac aaaaatggca atggtccaac cgaaatcgca aaaagctcgt ttatttatta
    25381 ctcacctgct actgctactt tttatcgcag cgattatgtt cccgctgctg atggtcgtcg
    25441 ctatctcgct gcgtcaggga aactttgcga ccggcagcct gatcccggag caaatctcct
    25501 gggatcactg gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc
    25561 caccgccatt cccggtactg ctgtggctgt ggaactcggt aaaggtcgcc gggatttccg
    25621 cgattggcat tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc
    25681 caggcaaagc gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt
    25741 cactggtcgc gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc
    25801 tgaatactca cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga
    25861 ccatcaaagg ctatttcgaa accatcgaca gttcgctgga agaagctgct gcgctggatg
    25921 gtgcgacacc gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg
    25981 cggtggtatt catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt
    26041 tactgcgtga cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc
    26101 aaaactacct gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca
    26161 tcgtcttctt gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag
    26221 gttaaagatg ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac
    26281 ttgtgactgt tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat
    26341 cgttcgccgg ttaatatttc catcatagag cttattattt ttacgttatt tgttttccca
    26401 cttacgataa ttctctttcg tgctctgagt cacggcaata gtattgtttt tatcaatttt
    26461 ggataattat cacaattaag atcacagaaa agacattacg taaacgcatt gtaaaaaatg
    26521 ataattgcct taactgcctg acaattccaa catcaatgca ctgataaaag atcagaatgg
    26581 tctaaggcag gtctgaatga atacccagta taattccagt tatatatttt cgattacctt
    26641 agtcgctaca ttaggtggtt tattatttgg ctacgacacc gccgttattt ccggtactgt
    26701 tgagtcactc aataccgtct ttgttgctcc acaaaactta agtgaatccg ctgccaactc
    26761 cctgttaggg ttttgcgtgg ccagcgctct gattggttgc atcatcggcg gtgccctcgg
    26821 tggttattgc agtaaccgct tcggtcgtcg tgattcactt aagattgctg ctgtcctgtt
    26881 ttttatttct ggtgtaggtt ctgcctggcc agaacttggt tttacctcta taaacccgga
    26941 caacacagtg cctgtttatc tggcaggtta tgtcccggaa tttgttattt atcgcattat
    27001 tggcggtatt ggcgttggtt tagcctcaat gctctcgcca atgtatattg cggaactggc
    27061 tccagctcat attcgcggga aactggtctc ttttaaccag tttgcgatta ttttcgggca
    27121 acttttagtt tactgcgtaa actattttat tgcccgttcc ggtgatgcca gctggctgaa
    27181 tactgacggc tggcgttata tgtttgcctc ggaatgtatc cctgcactgc tgttcttaat
    27241 gctgctgtat accgtgccag aaagtcctcg ctggctgatg tcgcgcggca agcaagaaca
    27301 ggcggaaggt atcctgcgca aaattatggg caacacgctt gcaactcagg cagtacagga
    27361 aattaaacac tccctggatc atggccgcaa aaccggtggt cgtctgctga tgtttggcgt
    27421 gggcgtgatt gtaatcggcg taatgctctc catcttccag caatttgtcg gcatcaatgt
    27481 ggtgctgtac tacgcgccgg aagtgttcaa aacgctgggg gccagcacgg atatcgcgct
    27541 gttgcagacc attattgtcg gagttatcaa cctcaccttc accgttctgg caattatgac
    27601 ggtggataaa tttggtcgta agccactgca aattatcggc gcactcggaa tggcaatcgg
    27661 tatgtttagc ctcggtaccg cgttttacac tcaggcaccg ggtattgtgg cgctactgtc
    27721 gatgctgttc tatgttgccg cctttgccat gtcctggggt ccggtatgct gggtactgct
    27781 gtcggaaatc ttcccgaatg ctattcgtgg taaagcgctg gcaatcgcgg tggcggccca
    27841 gtggctggcg aactacttcg tctcctggac cttcccgatg atggacaaaa actcctggct
    27901 ggtggcccat ttccacaacg gtttctccta ctggatttac ggttgtatgg gcgttctggc
    27961 agcactgttt atgtggaaat ttgtcccgga aaccaaaggt aaaacccttg agga
//
LOCUS       DACAPB010000043        27747 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.043, whole genome shotgun sequence.
ACCESSION   DACAPB010000043 DACAPB010000000
VERSION     DACAPB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.043"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..554)
                     /gene="fbaA"
                     /locus_tag="IT904_001670"
     CDS             complement(<1..554)
                     /gene="fbaA"
                     /locus_tag="IT904_001670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP3191464.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDG"
     gene            complement(769..1932)
                     /gene="pgk"
                     /locus_tag="IT904_001671"
     CDS             complement(769..1932)
                     /gene="pgk"
                     /locus_tag="IT904_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP3191465.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(1982..3001)
                     /gene="epd"
                     /locus_tag="IT904_001672"
     CDS             complement(1982..3001)
                     /gene="epd"
                     /locus_tag="IT904_001672"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAP3191466.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            complement(3286..3999)
                     /locus_tag="IT904_001673"
     CDS             complement(3286..3999)
                     /locus_tag="IT904_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAP3191467.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQAVSPQRRT
                     VVFLCAPPGTGKSTLTTLWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCAWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQAAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            complement(3996..4505)
                     /gene="fumE"
                     /locus_tag="IT904_001674"
     CDS             complement(3996..4505)
                     /gene="fumE"
                     /locus_tag="IT904_001674"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAP3191468.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNATLYDSLKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(4527..5492)
                     /gene="yggF"
                     /locus_tag="IT904_001675"
     CDS             complement(4527..5492)
                     /gene="yggF"
                     /locus_tag="IT904_001675"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP3191469.1"
                     /translation="MMSPAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(5489..6766)
                     /locus_tag="IT904_001676"
     CDS             complement(5489..6766)
                     /locus_tag="IT904_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP3191470.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHCSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(6781..8169)
                     /locus_tag="IT904_001677"
     CDS             complement(6781..8169)
                     /locus_tag="IT904_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAP3191471.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVEAVSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(8197..8640)
                     /gene="cmtB"
                     /locus_tag="IT904_001678"
     CDS             complement(8197..8640)
                     /gene="cmtB"
                     /locus_tag="IT904_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAP3191472.1"
                     /translation="MRVSDYFPESSISVIHSAKDWREAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGAIKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(8954..10945)
                     /gene="tkt"
                     /locus_tag="IT904_001679"
     CDS             complement(8954..10945)
                     /gene="tkt"
                     /locus_tag="IT904_001679"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP3191473.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            11223..11981
                     /locus_tag="IT904_001680"
     CDS             11223..11981
                     /locus_tag="IT904_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP3191474.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDEQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(12187..13107)
                     /gene="speB"
                     /locus_tag="IT904_001681"
     CDS             complement(12187..13107)
                     /gene="speB"
                     /locus_tag="IT904_001681"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAP3191475.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(13245..15221)
                     /gene="speA"
                     /locus_tag="IT904_001682"
     CDS             complement(13245..15221)
                     /gene="speA"
                     /locus_tag="IT904_001682"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAP3191476.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(15230..15361)
                     /gene="yqgB"
                     /locus_tag="IT904_001683"
     CDS             complement(15230..15361)
                     /gene="yqgB"
                     /locus_tag="IT904_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAP3191477.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            15497..15712
                     /locus_tag="IT904_001684"
     CDS             15497..15712
                     /locus_tag="IT904_001684"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191478.1"
                     /translation="MDITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            16016..17170
                     /gene="metK"
                     /locus_tag="IT904_001685"
     CDS             16016..17170
                     /gene="metK"
                     /locus_tag="IT904_001685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP3191479.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            17606..19000
                     /locus_tag="IT904_001686"
     CDS             17606..19000
                     /locus_tag="IT904_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP3191480.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            19077..19574
                     /locus_tag="IT904_001687"
     CDS             19077..19574
                     /locus_tag="IT904_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAP3191481.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            19669..20376
                     /gene="endA"
                     /locus_tag="IT904_001688"
     CDS             19669..20376
                     /gene="endA"
                     /locus_tag="IT904_001688"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAP3191482.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            20456..21187
                     /gene="rsmE"
                     /locus_tag="IT904_001689"
     CDS             20456..21187
                     /gene="rsmE"
                     /locus_tag="IT904_001689"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP3191483.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            21200..22150
                     /gene="gshB"
                     /locus_tag="IT904_001690"
     CDS             21200..22150
                     /gene="gshB"
                     /locus_tag="IT904_001690"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAP3191484.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRMKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            22187..22822
                     /locus_tag="IT904_001691"
     CDS             22187..22822
                     /locus_tag="IT904_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAP3191485.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            22822..23238
                     /gene="ruvX"
                     /locus_tag="IT904_001692"
     CDS             22822..23238
                     /gene="ruvX"
                     /locus_tag="IT904_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP3191486.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(23414..24394)
                     /locus_tag="IT904_001693"
     CDS             complement(23414..24394)
                     /locus_tag="IT904_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAP3191487.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLTAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            24412..25116
                     /gene="yggS"
                     /locus_tag="IT904_001694"
     CDS             24412..25116
                     /gene="yggS"
                     /locus_tag="IT904_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAP3191488.1"
                     /translation="MNDITHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            25134..25700
                     /gene="yggT"
                     /locus_tag="IT904_001695"
     CDS             25134..25700
                     /gene="yggT"
                     /locus_tag="IT904_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAP3191489.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            25697..25987
                     /locus_tag="IT904_001696"
     CDS             25697..25987
                     /locus_tag="IT904_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAP3191490.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            25995..26588
                     /locus_tag="IT904_001697"
     CDS             25995..26588
                     /locus_tag="IT904_001697"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAP3191491.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            26581..27717
                     /gene="hemW"
                     /locus_tag="IT904_001698"
     CDS             26581..27717
                     /gene="hemW"
                     /locus_tag="IT904_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAP3191492.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         6717 a         7420 c         6996 g         6614 t
ORIGIN      
        1 ccgtcttctt caccaccggt gcaacccagt tcgatttcca gagtcatgcc gattttggac
       61 atacgctcca ggtatttaga gcagatttcg atgttctctt gcagagattc ttcagacagg
      121 tcgatcatgt gagaagagaa cagcggctta ccggtagctg cgaagtgttt ttcacccgcg
      181 tccagcagac cgtcgatcca cggcagcagt ttcttcgcgc agtggtcagt gtgcaggata
      241 accggaacac cataatgttc agccatctgg tgaacgtgat gcgcaccaga gatcgcgccc
      301 aggatagcag caccctgcgg aacgtcagat ttcacgcctt taccagcgat aaaggaagca
      361 ccaccgttgg agaactgaac gataaccggc gctttaactt tagcagcagt ttccagtacg
      421 gcgttgatgg agtcagtacc gacgcagttt actgctggca gtgcgaagtt gttttctttt
      481 gctacctgga aaactttctg tacgtcatca ccagtgatta cgccaggttt tacgaaatca
      541 aaaatcttag acatgtctct tgtcctgtat cgtcgggcct tatactcatc atacttcaag
      601 ttgcatgtgc tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg
      661 attcactctc ttgtcgcctt cctgcaactc gaattattta gagtatgaaa aaatgcgtgc
      721 tctaaaagcg cgctgaaaca agggcaggtt tccctgccct gtgatttttt acttcttagc
      781 gcgctcttcg agcatcgcta ctgcaggcag tactttacct tccacgaatt cgaggaatgc
      841 gccgccgcca gtggagatgt aggagatttt gtcagcaatg ccgaacaggt cgattgctgc
      901 cagagtgtcg ccgccgccag cgatggagaa cgcttcgctg tctgcgatag cgttagccac
      961 gatttcagta cctttgcgga agttcgggaa ttcgaacacg ccaaccggac cgttccacag
     1021 aatggttttc gcattcttca ggatttcagc cagttcctga gcggaagcat caccgatatc
     1081 caggatctgc tcgtcagctt tcacatcgtt aacagatttc agggtagccg gtgcagtttc
     1141 agagaactcg gttgctacgc gaacatcaga cggaaccggg atgttgcagg tggtcagcag
     1201 acgtttagct tcgtcaacca ggtcagcttc gtacagggat ttacccacat cgtggccttg
     1261 tgccgcgata aaggtgttag cgataccacc accaacaatc agctggtcag cgattttaga
     1321 cagggagtcc aaaacggtca gtttggtaga tactttagaa ccaccaacga tagccaccat
     1381 cgggcgagca ggttctttca gtgctttacc cagcgcgtcc agttcagctg ccagcagcgg
     1441 gcctgcgcac gcaacgtcag cgaatttacc gataccgtga gtagaagcct gcgcgcggtg
     1501 agcagtaccg aatgcgtcca ttacgaacac gtcacacagt gcagcgtatt ttttggacag
     1561 ggtttcgtcg tctttcttct cgcctttgtt gaagcgaacg ttttccagaa caaccagttc
     1621 accttcagca acgtcaacgc cgtcgaggta atctttaacc agacgaaccg ggttagacag
     1681 tttgtctttc aggtagttaa caaccggcag cagagagaat tcttcgttgt actcgccttc
     1741 ggtaggacga cccaggtggg aagttaccat cacttttgcg ccttgtttca gggccagttc
     1801 gatggtcggc agagaagcac ggatacgcgc gtcgctggtt actttcccgt cttttactgg
     1861 tacgttcaga tccgcacgga taaatacacg tttcccagca agatccagat cggtcatctt
     1921 aattacagac atggtgaatc ctctcgttga ttctaaaagt tttgcagacg ctgcttgcgt
     1981 cttacctgaa agcaacagta gccatagcta acgtcgtgtc gagcattcgg ttagcaaagc
     2041 cccattcgtt atcgcaccag accaacgttt tgatcaggtg tgcgccacta acccgggttt
     2101 gagtgccatc gacaatggca ctgtgcggat cgtggttaaa atctacagag accaacggca
     2161 attccgtata gtcaactata ccatgaaatg caccttgtgc tgctttttgc agcaacaggt
     2221 tgacttcatt ggcttttaca ggtttcttta ccgtcacgct taaatcgatt gccgtcacat
     2281 ttatggttgg cacacgtacc gcaatcgcct caaagcgatc gttaaattgc ggaaaaaatc
     2341 gtgtgatacc ggcggccagt ttagtatcga ccggaatgat cgactggctg gctgcccggg
     2401 tgcgacgcag gtcaggatgg tatgcatcaa taacctgttg atcgtgcatg gcggagtgaa
     2461 ttgtggtcac agtgccggac tcaataccgt acgcatcatc taacaatttg atgacgggaa
     2521 ttatgcaatt cgtggtacag gaagcgttag aaacgatgcg gtgttccgca cgaagttgat
     2581 cctgattgac gccgtaaaca acggtcgcgt cgagatcgtt actgccagga tgtgaaaaga
     2641 gcactttttt ggccccggcg gcaatatgcg cttcgccatg ctcgcgggag ccatatacgc
     2701 cggtgcagtc gaggactaca tcaacgccaa gttcacgcca ggggagcgat tgcagtgaac
     2761 gttcatgcaa tacgcggatg gcgtcatcac caacaaaaag ttgatcgcgt tcctgtcgta
     2821 cttcccatgc aaaacggcca tggcttgtgt catatttcaa caaatgcgcc atgcccgcag
     2881 catccgccag ttcgttgatt gccaccacgg taatttccgc ccggcgtccg gattcataca
     2941 aagcacgaac cacattacgc ccgatgcgac cgaagccatt tatcgctacg cgtacggtca
     3001 tagatctcct gcaaggtttt ccctgagcaa atttgccaga cagagtaatc cagcaaatcg
     3061 tccggggaaa ccttacctgt cgcaaactgc gactgattgg ttaattgtcg aacatttaat
     3121 cgactgaaac gcttcagcta ggataagcga aacgtggaat aaaaggaatg tttgtccagc
     3181 cgaagaagac atttatctga ctcacatcac acttttatcc ccttttgtgg gaagctttat
     3241 tccaggctgg cgtaataata accctgcaat aactggaata aattgtcagc ttgtgaagtg
     3301 ataccggcct tcttcagtca tctctactat cagattggcc tgacggctat tcatcagcac
     3361 acgctcgaca ttggggccat cggtgcgggc ataaaatgct tcggctgctt gccgcgataa
     3421 tcctcccgca attttacgac taatcaagcg ctcccgcaga atttgagcgg gtgcatgaat
     3481 aaaaatagaa aaatcgcaga aagatgccaa ctccagccac ttttcatcat ccagtaatag
     3541 ccagtttcct tcgacgatga cgagtggtgc ggtaacgtgc aacgcatctt caacaggatc
     3601 atgcttttgt cgatcatact gcggccacgc acaatcccct tccactacct ggcgcagatt
     3661 ttccgccagt ttcgctacat cgaatgtctc tggtgcgcct ttgaaggggc gcaattgatg
     3721 cgcatccagc cagctattgt aatggtgaaa accatccatc gggagtgtct gaatagcggg
     3781 cagttcgggg tcttgttgcg cgagatattc ccagagggtg gttagggttg atttacccgt
     3841 ccccggcgga gcgcaaagaa aaaccaccgt acgtcgctgc ggacttaccg cctgtaaggc
     3901 cgccagcata cgcagtagcg gtttatgaac attctcaatt tcatcatcag agtactgcgc
     3961 ctgtaccttt agtccattca ccgtcagttc gattttcacg gcgttaattc ctttaaagtt
     4021 gtcgttactg ccagcgtcag cgccgttttg accatcccgc taaaacgcgc ctctgaccag
     4081 acggcgaaat cggcgaactt catcgctttc aacgagtcat agagcgtcgc attgcgggtt
     4141 atactattga cgttgctgat aaaatcccag gtcatgtcat cggcaaaccc gggcgtttca
     4201 tcctgttcgt tcaggtaatg ccagaactgg gaaaaatgcg tgatatcggc gtacaaccat
     4261 ttatccattt tccccaacgc ataaatcaaa cgcagtgcaa catcaatatc gtcaagcggg
     4321 ccgctttggg cgagtaacgg cttcactgca tattccacga tctcttcatc gttatcgaca
     4381 aacagcgacg gcagaaactg gcgtatcccc tggagcaaaa tggtatgcgc agttttcata
     4441 aatgaaaata aattgtcctg tgcatccagt tgctcaagca catcatcttc tgtcagcgtc
     4501 gccattggtc ttccgataga tcaccatcaa tgcagggagt ctattatatt acacgtttgg
     4561 tccgcgccgc cgatcagtaa tgtctgcgtc cgcaccccat tcgccgtctg ttggataccg
     4621 ttcaccagct caccgcccgt cacgcccgtt gcgctaaaga ggatatcgtt ccccctcacc
     4681 agttcatcga gcgagtacac acggttaacg tcaacgccca ttgccttgca acgcttgcgc
     4741 tcctgctctg caatctgccg attttccgtg taatcccctt tagcctgaca aaagtcaatc
     4801 agctcggcct gcatatcccc gcctaatgct ttaacggcac aggcagaaat cacgccttct
     4861 ggcgcaccgc cgatggtgta catcacatcg taaggattat cctgccagca ggtcagcacg
     4921 ctggcggcga catcgccatc cggcagggca aaaaccttca cgcccagctg tgttgcttcc
     4981 tcaatcgcgg cactcaggcg cggtttatcc agcgtaacca tgcgcagctt atccagcggt
     5041 ttgcctaacg ctttcgccac gttgcgcagg ttgtccgtca gaggcaaaga cagatcaatc
     5101 gcgcccgccg ccagccgatt aacaaccagc tttttcatat acatatcggg cgcatgtaac
     5161 aggctatctc gaggggcgaa agccatgacc gccagcgcat tgctctgccc catcgccacc
     5221 atccgcgtgc cttcaatggg atcaaccgcg atatcgactt ctggcccatc gcctttgccc
     5281 acctcttcgc caatccacag catcggcgca tggtcaattt ctccttcgcc gataaccact
     5341 cgtccacgaa aagcgacgtc gtttaatgcc tggcgcatcg cggtgaccgc caggccatca
     5401 attttatttt tgtcgccaca tccggtttgc ggccaggcag ccaacgctgc ctgttccgta
     5461 acgcgaaata atggccacgc cggggacatc attgcgcggc ctccccgata tcaacgccaa
     5521 attgtgccag cagatatttc tcggcctgtt cattccagat gccgtgcgtt tcctcaacca
     5581 gccgcgccaa ctctttaaac agcggatcgg ttttgccttt ttcggcaaaa tcggcaatgg
     5641 cagtgagcgg catggtcacg ccgttataaa tgagtttttt accgccaggg atatccggca
     5701 gattgagcac ggtttctggc accgcatcca ggccaccgat atgggtcacc ataaacgacg
     5761 gctgtaactg cccagtggcg ctaagggcaa tcgcctcttt catgtcgtcc gttgaaccgc
     5821 cagatgtacc gacgacgtgc gtgctgttgt aatggacgtt gtagaaatta aacggcactt
     5881 tgaagttttt atccgtcggc ccggcaaaga agttcagaca gccatcttcc gccagtaatt
     5941 catcagccat ctcaacgaca gcaggcaccg ccgcataaac aaaaatgtca tcgaacccgg
     6001 catctcctgt cagcgcccgc agcatctgga caggatcgct catccctttg gtattcacat
     6061 acaccagctc aatgccttta ctggccgcca gttccaccgg cagcagcttc tgtacctgcg
     6121 ccagacgttt gtcgtcgata tcgaccacca ccacccgcga cggttgtatg ccgccgttaa
     6181 tggcgtaatc gatagcgcca atgcccatcg gacctgcaca cgccagcagt gcaatattgc
     6241 cgccaggttt gacgcccatg cgatgctcat aaacatattg cgtggtgtga taattggcat
     6301 gataagcacc aataatgcag cacataggct ctgccagcga cgccgcagca aaataagagc
     6361 agtgatacgg taatacgcag cccaaattaa tggcgatttc gggaataatc atataagtgg
     6421 cattgccgcc aaaatattcg tagctgtagc ccgctgaata tccgcttggt aaccccatcg
     6481 ccggttgcaa tacaaaacgc tggccttttt tatatttgcc agtgagattt ttacccactt
     6541 cgacaataac cccggcacat tcatgcccgg taatgaccgg atgattttct aaatcgtcgg
     6601 gtacgcgttt atgttcacta ccgagtaacg ccgctttcca ggtcgataaa cagacgctgt
     6661 cagaaattac actcaccagt aattcattat cggtaatttc tggcagttca aatacgcgca
     6721 gacggacatc ccgcttgcca taaatagcag caactttggt tttcattttt acctctgtaa
     6781 tcagtgttta tgttcggcgg ttaattgatt aaataaagtg tcgagttttg gatcgccaat
     6841 atagttatta atcaatatca gtggtttatc cgtcacacgc ttcacgcgcc cttccagact
     6901 ggcatgagta acgacgatat ccgcatccgc aggcacgttt tctatggcgt aatgttttac
     6961 ttcaattgcc agccccgctt tttccaggcg tttacggaag gtggtcgcgc ccatcgcact
     7021 ggagcccatc cccgcatcgc aaacaaaggc gatacgctta accctgctta acgagaatgc
     7081 accctcttgt ttcatcgcct taaccgcatt ggctgactga gcaaactcat cttcgctcac
     7141 cgcttccacc gttttttcca tcttcagtat cagcgaagtg atagcaaagg acaccagggt
     7201 acctaccgta acaccggcaa ttgtcgccag gaacgaacct ttcggcgtca gcgccaggta
     7261 agcaaagata gaccccggac ttgggccagc caccagacca ccgtccagta agttaaacat
     7321 ccaggtgcca gacataccgc ccgcaatcat ggcaataata gtcagcggct tcatcagcac
     7381 atatgggaaa tacagttcgt ggatcccacc gaggaagtga ataatcatcg ccccgggcgc
     7441 agaacgttta ctcatccctt taccaaacaa ggtaaacgcc agcagcagcc ccaggcccgg
     7501 acctgggtta gaggccacca taaagaagat ggatttaccg ttaaccgaag cctgttgcat
     7561 tcccagcgga taatagacgc cctgatcgat cgcattatta aggaaaagca ctttcgccgg
     7621 ttcattgatg actgacagca gaggcagata acccgcatgt accagcgcct caatacactc
     7681 tttgacgaaa gtattggcaa ttaacaccgc cgggccgatc acttcaaaac ccagcagaca
     7741 aaggagcatc cccgcgatac ctaatgagaa gttattgata accatctcaa aaccagcagg
     7801 tatgcgtttt tccagtgcct tatcaacata ttttatgacc agcccaccga gcggccccat
     7861 aatcattgag ccaaggaaca tcgggatctc tgcgccgacg atcacaccaa tagtacctat
     7921 tccgcccatg accgcgccgc gtttaccgcc gaccagatga ccaccggtag aaccaatcat
     7981 cacgggcaat aaataggtaa tcatcgggcc gacaattttg gcgaaatgtt cgttaggcag
     8041 ccaaccggtg ggaataaata acgcagtaat aaaaccccag gcaataaaag caccaatatt
     8101 ggggatgacc attgccgtca aaaatccccc aaaagcctgg acctttgcac gagcagactt
     8161 gttttccata atattatcct gtagaggaga gagtaattat ccgcggctga taatgtccgc
     8221 taattgtttt tctgatgatg ctgttaagag ttgttcgagt atttcttctt cgcacagtaa
     8281 ttcacttaac gcctgaatag cgccaatgtg cgaatcggca tcggcagcag agagtccgat
     8341 gagtaattta attggttcgt cattccccgg aaaataaaca ccttgttcaa gtaatgtcaa
     8401 agacatcccg gttttaatcg ccccacattc cggtcgcgca tgaggcattg ccacgcctgg
     8461 tgcgagaata taataagggc cattgttaat ggtggaatct ttaattgcct gaatgtaatt
     8521 ctcgctgata tagtttttat caagcaatga taccatcgag aaatcgatag cttcccgcca
     8581 atcttttgct gaatgtataa ctgagattga tgactctgga aaataatcac taacccgcat
     8641 aaattatcct tattttattt ttagggtacc gtccggcggt aaaaataaaa tactcgtgag
     8701 atttaatgaa agcaattgag cagggaaacc ggtgttttga aaacagtgaa ttattaattt
     8761 cttatataac attgagttat agatataaca acaaatgtca acacgcatat cgtgatgcat
     8821 attacataat tgatatttat tgaatataaa tatttgtgat ctacaacacg ctttatctat
     8881 tgcttgtccg caaacggaca tatcaaggta ataaaaaagg tcgccgaagc gaccttttta
     8941 cccgaaatgc taattacagc agttcttttg ctttcgcaac gacgttgtcg acggtgaagc
     9001 cgaactcttc gaacagcaac tctgccggag ctgactcacc gaaggtggtc ataccgacga
     9061 tagcaccgtt caggccaaca tacttgtacc agtagtcagc aatgccagct tctactgcaa
     9121 cacgagcagt aaccgctttc ggcaggacag actcacggta agccgcgtcc tgcttgtcga
     9181 atgcgtcggt agacggcatg gaaaccacgc gcgctttcac gccttcggca gtcagttttt
     9241 cgtaggcagc aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct
     9301 gaccagcaca gtctttcagc acataaccac cgcgcgcgat gtttgccagt tgctcttcag
     9361 tacgttcctg ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac
     9421 gctcaacgcc gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag
     9481 acatgttcgg ggtcacgcgc agagaagcga cctgctcaac cggctggtga gtcgggccat
     9541 cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt ttcatcagcg
     9601 cagccatacg tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg
     9661 gcaggaaacc accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa
     9721 caccgtagtg gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca
     9781 gggtcaggtt agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc
     9841 cgaacgcttc gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct
     9901 gcagtttagc gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca
     9961 tacggcgggt aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct
    10021 cattccatgc agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt
    10081 cagacgggat ttcgaacggc gcgtatttcc agcccagctg ttcgcgggtc agggcaattt
    10141 cagcgtcgcc cagcggcgca ccgtgggagt cgtgggtacc ggctttgttc ggggaaccga
    10201 aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt
    10261 ctactgcgcg tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc
    10321 cgtaagcttc gaaacgcatt gcggtgtcgt cagtgaacca gccttcaacg tgaccatcga
    10381 ttgagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca
    10441 gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg
    10501 tgtagtggtc gacaatgtcg tgacctggac ggttaaactg cgccgccagc gttttttctg
    10561 caatcgccat accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca
    10621 caccagcggt ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt
    10681 ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca
    10741 tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg
    10801 ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg
    10861 cacccgggtg accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat
    10921 tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct
    10981 taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc
    11041 aagccgcgcg tcatgtacat ggaacatcct tttgctgctt cagaaatctc tggatcatgc
    11101 tcgcatgttg cgcaatctac tcgcccgtcc gttgcgcttt tccttatact gagactgagc
    11161 gtcgattcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa
    11221 acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc
    11281 agaatatgga ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt
    11341 tgagtgatga gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg
    11401 cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc
    11461 taggcaacaa tatcaacggt cagccggtaa attacaaagt gtatatggcg aaggatgtga
    11521 acgcctttgc aatggctaac ggctgtatcc gtgtctatag cgggctgatg gatatgatga
    11581 cggataacga agtcgaagcg gtgatcggtc acgaaatggg gcacgtggca ttaggccatg
    11641 tgaaaaaagg aatgcaggtg gcacttggta caaatgccgt gcgagtagct gcggcctctg
    11701 cgggcgggat tgtcggcagt ttatctcaat cacaactcgg tgatctgggc gagaaattag
    11761 tcaattcgca attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc
    11821 tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcaaaac
    11881 tggaagaagg tcgccaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgcgccc
    11941 agcatattcg cgatcgtatg agcgcagatg ggattaagta aagcctggtg gtgtcgaacg
    12001 taggtcagat aaggcgttca cgccccttct gacatgaatg gcacatttgt taccctgtgc
    12061 gcattgccgg atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg
    12121 gataaggcgt tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc
    12181 tggtgcttac tcgccctttt tcgccgcctg aatatagagc atctccagcg ccagcgtcgc
    12241 cgcagccaga gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat
    12301 cccaacgatg ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag
    12361 gccgccaatc actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc
    12421 aaaggtcagg taaaccggca tatcacccac aatctgtttc acttgggcga taacgtcatc
    12481 cacgctgcga tcgttcacct ggcaggcgtc cagcacggta aaaccgttgt ctttatcaaa
    12541 ctcggtacga ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc
    12601 ggtatagaac atggtgccgt ggtcaaattc acaaccgttc gcgtaggtat cggtgtgggc
    12661 gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag
    12721 cgtaacaaag tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc
    12781 ggcgtgcgcc tgcagcttct cgctcatctc acgggcatcg ccaaaggcat ataccagatc
    12841 gccgcagtcc acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg
    12901 ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc
    12961 acggccagag gtggccatgt cgaacggcac gccggtgatc acccagtcgg catcgctgtc
    13021 atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag gcgttggaaa ccagtgagtt
    13081 atcgtattga tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa
    13141 aacccttccg cgtcgttagg cccgacgagg aagggttgga tttgtcacaa taaattgtgg
    13201 cggattatca ccgctaaatt aagcggattc aagtaacaca ggacttactc atcttcaaga
    13261 taagtataac cgtacaaacc tgcctcgaac tcttcaagga actgttgttg cagttcagca
    13321 tcaagatcgg ttttcttcac ttgatcgcgg aactgggtta acagcgtttt cggatcgagc
    13381 tgtacatatt gcagcatgtc cgccacggta tcgccttcgt cagacagttc tacttctacg
    13441 ctaccgtcag ggaacacgaa cacgtcaacc gcttcggtat caccgaacag gttgtgcata
    13501 ttgccaagga tctcctgata tgcgccgacc ataaagaaac cgagcatcgg cggattctct
    13561 ggatcgtact ccggcattgg cattgtcgtg gcaataccat caccatcaat atagtggtcg
    13621 atagcaccgt cagagtcaca ggtaatatcc agcagcacag cgcgacgttc cggcacttga
    13681 tccagccctt ccagcggcag aaccgggaac aactggtcga tcccccatgc gtccggcatt
    13741 gactggaaca gcgagaaatt gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg
    13801 attatcggac gatgcgcacg gttttgcgga tccagttgct tctgcacttc atggcacatg
    13861 ctcagataga gttgttctgc ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag
    13921 ccgatatgaa tgtcgtgcaa atccatctga ctgtcgtgta accattcacg cagagaacgg
    13981 cgagttcccg gttcgtgcat ctcctgccag gtttcccaca tgctttgcag cgcgcgcggc
    14041 gcatcttctg caggcgcggt cggcaccgtg tattcgttac gttccacgcc gatgatatta
    14101 gacaccagca cggtgtgatg cgcagtcacc gcacgacccg attcggtgat taccgtcgga
    14161 tgcggcagac cgttttcttc acacgcatca ccaatcgccc agatgatgtt gttggcatat
    14221 tcattgaggc cgtagtttac ggagcagtca gactgcgaac gggtgccttc atagtccacg
    14281 cccagaccgc cgccgacgtc gaagcactga atattgacgc ccagcttgtg cagttccaca
    14341 tagaaacgcg ccgattcacg aacgcctgtg gcgatatcgc gaatattcgc catctgcgaa
    14401 ccgaggtgga agtgcagtag ttgcaggctg tcgagacgcc cggcttcacg cagggtttca
    14461 accagttgca gtacctgagt cgcagccagg ccgaacttcg atttttcccc gccggaggac
    14521 tgccatttac ccgagccctg cgaagccaga cgtgcacgca cgcccagacg aggaacgaca
    14581 ttcagacgtt ctgcttcatc cagcacaatg gcgatttctg acatcttctc aatgaccagg
    14641 tagaccttgt ggcccatctt ctcgccaatt aatgccaggc ggatatattc gcggtcttta
    14701 taaccgttgc agacgatgac gctacgggtc atgccagcat gtgccagcac cgccatcaac
    14761 tctgctttcg agcctgcttc cagtcccagc ggttcgcccg aatgaatcag ggactcaatc
    14821 acgcggcggt gctggttaac tttgatcgga taaacaagga agtaatcgcc gttatagccg
    14881 taggattccc tcgcacgttt gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc
    14941 tgtgggaaac agaacagtgc aggcagacgc tgaccctgtg cttcacgagt tttcactaac
    15001 tgcgcgagat cgacgcgagc ttccgggacg tccgggtccg ggcacacgct gatgtggccc
    15061 agctcgttaa cgtcatagta gttattgccc caccaggcaa tattgtaagt acgcagcatc
    15121 ttgctggctt cctgggagct cattgcaacc tcctgcatgg agcgtagtac accgtgttcg
    15181 cccgctgacg aaggcaaacc catagacatg tcgtcagaca tagcgaacct caaattattt
    15241 tattaagtgt aaaacagtta acgactatcg cagcctggaa ctgcgataac aacccataag
    15301 cacatggatt ttccagcagt gaatgctgac gctccaactg cgcaaccggt ttctttttca
    15361 taacattatt aagcacataa ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa
    15421 ctcttgtttt tacaagagcg cccttgttca gtcctcagta actgtaacca gctcttgaat
    15481 cctgagaagc gccgagatgg atataacatc ggcaggtatg caaagcagag atgcagagtg
    15541 cggggaacga atcttcacca gaacggtgag acaggttaag cagcagacaa cggttcatta
    15601 tttcgtatca cctccacggc cgcctgttaa gacgaaccca caagccaaaa ctctgatttc
    15661 aacccggctg gaagtggcaa cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg
    15721 ccgcgtttta taccccacaa tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc
    15781 attgtcagga aaaatttcca gccacgtttt taacagaatg agacacgatt caaaaaaaag
    15841 tggaaatcgg gtgaagaatt gacctaaaat agccatccag atgttaatcc atccataccg
    15901 attaacactc agactgccag tgtttttaac ctgcagagtc gtggtaggat ccgctaccac
    15961 agaaaatcca cacaacagtt tgagctaacc aaattctctt taggtgatat taaatatggc
    16021 aaaacacctt tttacgtccg agtccgtctc tgaagggcat cctgacaaaa ttgctgacca
    16081 aatctctgat gccgttttag acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg
    16141 cgaaacctac gtaaaaaccg gcatggtttt agttggcggc gaaatcacca ccagcgcctg
    16201 ggtagacatc gaagagatca cccgtaacac cgttcgcgaa attggctatg tgcattccga
    16261 catgggcttt gacgctaact cctgtgcggt tctgagcgct atcggcaaac agtctcctga
    16321 catcaaccag ggcgttgacc gtgccgatcc gctggaacag ggcgcgggtg accagggtct
    16381 gatgtttggc tacgcaacta atgaaaccga cgtgctgatg ccagcaccta tcacctatgc
    16441 acaccgtctg gtacagcgtc aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg
    16501 cccggacgcg aaaagccagg tgactttcca gtatgacgac ggcaaaatcg ttggtatcga
    16561 tgctgtcgtg ctttccactc agcactctga agagatcgac cagaaatcgc tgcaagaagc
    16621 ggtaatggaa gagatcatca agccgattct gcccgctgaa tggctgactt ctgccaccaa
    16681 attcttcatc aacccgaccg gtcgttttgt tatcggtggc ccgatgggtg actgcggtct
    16741 gactggtcgt aaaattatcg ttgataccta cggcggcatg gcacgtcacg gtggcggagc
    16801 attctctggt aaagatccat caaaagtgga ccgttccgca gcctacgcag cacgttatgt
    16861 cgcgaaaaac atcgttgctg ctggcctggc cgatcgttgt gaaattcagg tttcctacgc
    16921 aatcggcgtg gctgaaccga cctccatcat ggtagaaact ttcggtactg agaaagtgcc
    16981 ttctgaacaa ctgactctgc tggtacgtga gttcttcgac ctgcgcccat acggtctgat
    17041 tcagatgctg gatctgctgc acccgatcta caaagaaacc gcagcatacg gtcactttgg
    17101 tcgtgaacat ttcccgtggg aaaaaaccga caaagcgcag ctgctgcgcg atgctgcagg
    17161 tctgaagtaa tctttcttca cctgctttca aaggccagcc tcgcgctggc ctttttcttt
    17221 tacctcaccg cggtggatag gcattcacgc cgcatccggc aaaaagctgc ccgcacaata
    17281 acatcattct tcctcaacac gttttatcgc aaattatcat cactactgaa accgattaca
    17341 ccaataacac cagccaaata tttgtaatat tttcatatta ttattcagtt ttcacagttg
    17401 ttacatttct tttcagtaaa gtcttaattg cagataacag cgtttaatct atgatgatat
    17461 aactcaatta ttttcatgca cttaaaccat aactaagata aatgttagtg taagcgatta
    17521 cactgatgtg atttgcttca catcttttta cgtcgtactc acctatctta attcacaata
    17581 aaaaataacc atattggagg gcatcatgcc tgacgctaaa aaacaggggc ggtcaaacaa
    17641 ggcaatgacg tttttcgtct gcttccttgc cgctctggcg ggattactct ttggcctgga
    17701 tatcggtgta attgctggcg cactgccgtt tattgcagat gaattccaga ttacttcgca
    17761 cacgcaagaa tgggtcgtaa gctccatgat gttcggtgcg gcagtcggtg cggtgggcag
    17821 cggctggctc tcctttaaac tcgggcgcaa aaagagcctg atgatcggcg caattttgtt
    17881 tgttgccggt tcgctgttct ctgcggctgc gccaaacgtt gaagtactga ttctttcccg
    17941 cgttctactg gggctggcgg tgggtgtggc ctcttatacc gcaccgctgt acctctctga
    18001 aattgcgccg gaaaaaattc gtggcagtat gatctcgatg tatcagttga tgatcactat
    18061 cgggatcctc ggtgcttatc tttctgatac agccttcagc tacaccggtg catggcgctg
    18121 gatgctgggt gtgattatca tcccggcaat tttgctgctg attggtgtct tcttcctgcc
    18181 agacagccca cgttggtttg ccgccaaacg ccgttttgtt gatgccgaac gcgtgctgct
    18241 acgcctgcgt gacaccagcg cggaagcgaa acgcgaactg gatgaaatcc gtgaaagttt
    18301 gcaggttaaa cagagtggct gggcgctgtt taaagagaac agcaacttcc gccgcgcggt
    18361 gttccttggc gtactgttgc aggtaatgca gcaattcacc gggatgaacg tcatcatgta
    18421 ttacgcgccg aaaatcttcg aactggcggg ttataccaac actaccgagc aaatgtgggg
    18481 gaccgtgatt gtcggcctga ccaacgtact tgccaccttt atcgcaatcg gccttgttga
    18541 ccgctgggga cgtaaaccaa cgctaacgct gggcttcctg gtgatggctg ctggcatggg
    18601 cgtactcggt acaatgatgc atatcggtat tcactctccg tcggcgcagt atttcgccat
    18661 cgccatgctg ctgatgttta ttgtcggttt tgccatgagt gccggtccgc tgatttgggt
    18721 actgtgctcc gaaattcagc cgctgaaagg ccgcgatttt ggcatcacct gctccactgc
    18781 caccaactgg attgccaaca tgatcgttgg cgcaacgttc ctgaccatgc tcaacacgct
    18841 gggtaacgcc aacaccttct gggtgtatgc ggctctgaac gtactgttta tcctgctgac
    18901 attgtggctg gtaccggaaa ccaaacacgt ttcgctggaa catattgaac gtaatctgat
    18961 gaaaggtcgt aaactgcgcg aaatcggcgc tcacgattaa tctcctcagg cttcctccca
    19021 tcgcggagga agccacctct tgcagtcatc ttttcttcgc tctatcctct gccgctatga
    19081 aaacatcccg tctccctatc gccatccagc aggccgttat gcgccgcctg cgggaaaaac
    19141 tcgcccaggc caacctgaag ctagggcgta actacccgga gccaaaactc tcttacaccc
    19201 agcgcggaac ctccgccgga acagcctggc tggaaagcta tgaaatccgc ctcaatcccg
    19261 ttttgctgtt ggaaaacagt gaagctttta ttgaagaagt ggtaccgcac gaactggcac
    19321 atttgctggt atggaaacat ttcggccgcg tagcgccaca tggcaaagag tggaagtgga
    19381 tgatggaaag cgtgctgggc gttcccgccc gtcgtacgca tcagttcgaa ctgcaatccg
    19441 tgcgtcgcaa caccttcccc taccgctgca agtgccagga gcatcagctt accgtacgcc
    19501 gccataatcg cgtagttcgt ggcgaggccg tctatcgctg tgttcactgc ggtgaacagc
    19561 tggttgcgaa ataaccatct gaactatcag gaactttcct gatctggctg attgcatacc
    19621 aaaacaactt tcgctacgtt gctggctcgt tttaacacgg agtaagtgat gtaccgttat
    19681 ttgtctattg ctgcggtggt actgagcgca gcattttccg gcccggcttt ggccgaaggt
    19741 atcaatagtt tttctcaggc gaaagccgcg gcggtaaaag tccacgctga cgcgcccggt
    19801 acgttttatt gcggatgtaa aattaactgg cagggcaaaa aaggcgttgt tgatctgcaa
    19861 tcgtgcggct atcaggtgcg caaaaatgaa aaccgagcca gccgcgtaga gtgggaacac
    19921 gtcgttcccg cctggcagtt cggtcaccag cgccagtgct ggcaggacgg tggacgtaaa
    19981 aactgcgcta aagatccggt ctatcgcaag atggaaagcg atatgcataa cctgcagccg
    20041 tcagtcggtg aggtgaatgg cgatcgcggc aactttatgt acagccagtg gaatggcggc
    20101 gaaggccagt acggtcaatg cgccatgaag gtcgatttca aagaaaaagc tgccgaacca
    20161 ccagcgcgtg cacgcggtgc cattgcgcgc acctacttct atatgcgcga ccaatacaac
    20221 ctgacactct ctcgccagca aacgcagctg ttcaacgcat ggaacaagat gtatccggtt
    20281 accgactggg agtgcgagcg cgatgaacgc atcgcgaagg tgcaaggcaa tcataacccg
    20341 tatgtgcaac gcgcttgcca ggcgcgaaag agctaaccta cactagcggg actctttttg
    20401 ttaaccccta ccccacgcgt acaaccgcgt ggggagacga cgcggatttt taactatgcg
    20461 tatcccccgc atttatcatc ctgaaccact gaccagccat tctcacatcg cgctttgcga
    20521 agatgccgcc aaccatatcg ggcgcgtact gcgcatgggg ccggggcagg cgttgcaatt
    20581 gtttgacggt agcaaccagg tctttgacgc cgaaattacc agcgccagca aaaaaagcgt
    20641 ggaagtgaag gtgctggaag gcaagatcga cgatcgcgaa tctccgctgc atattcacct
    20701 cggtcaggtg atgtcgcgtg gtgaaaaaat ggaatttacg atccagaaat cgatcgaact
    20761 cggtgtaagc ctcattacgc cacttttttc tgagcgctgc ggcgttaaac tggatagtga
    20821 acgtctgaac aagaagcttc agcagtggca gaagattgca attgctgcct gtgagcagtg
    20881 tggtcgtaac cgggtgccgg aaatccgtcc agcgatggat ctggaagcct ggtgtgcaga
    20941 gcaggatgaa ggactgaaac tgaatcttca cccgcgcgcc agtaacagca tcaatacgtt
    21001 gcctttaccg gttgaacgcg tccgcctgct gattggcccg gaaggcggtt tatcggcaga
    21061 tgaaattgcc atgactgccc gctatcaatt tactgatatc ctgttgggac ctcgcgtttt
    21121 gcgtacagag acaactgcgc tcaccgccat taccgcgcta caagtacgat ttggcgattt
    21181 gggctaacgg agaagaataa tgatcaagct cggcatcgtg atggacccca tcgcaaacat
    21241 caacatcaag aaagattcca gtttcgctat gttgctggaa gcacagcgtc gtggttacga
    21301 acttcactat atggagatgg gcgatctgta tctgatcaat ggcgaagccc gcgcccatac
    21361 ccgcacgctg aacgtgaagc agaactacga agagtggttt tcattcgtcg gtgaacagga
    21421 tctgccgctg gccgatctcg atgtgatcct gatgcgtaaa gacccgccgt ttgataccga
    21481 gtttatctac gcgacctata ttctggaacg tgccgaagag aaagggacgc tgatcgttaa
    21541 caagccgcag agcctgcgcg actgtaacga gaaactgttt accgcctggt tctctgactt
    21601 aacgccagaa acgctggtta cgcgcaataa agcgcagcta aaagcgttct gggagaaaca
    21661 cagcgacatc attcttaagc cgctggacgg tatgggcggc gcgtcgattt tccgcatgaa
    21721 agaaggcgat ccaaacctcg gcgtgattgc cgaaaccctg actgagcatg gcactcgcta
    21781 ctgcatggcg caaaattacc tgccagccat taaagatggc gacaaacgcg tgctggtggt
    21841 ggatggcgag ccggtaccgt actgcctggc gcgtattccg caggggggcg aaacccgtgg
    21901 caatctggct gccggtggtc gcggtgaacc tcgtccgctg acggaaagtg actggaaaat
    21961 cgcccgtcag atcgggccga cgctgaaaga aaaagggctg atttttgttg gtctggatat
    22021 catcggcgac cgtctgactg aaattaacgt caccagccca acctgtattc gtgagattga
    22081 agcagagttt ccggtgtcga tcaccggaat gttaatggat gccatcgaag cacgtttaca
    22141 gcagcagtaa cccaccttag cgagaaggat ctcgttgaga ctctgagtga cagcgccctt
    22201 ctttccacgc atactgggcg ctgttgcttt tttgaaccag gaaacagaac ctctgacaat
    22261 gaatttacag catcactttc ttattgccat gcctgctctc caggatccga ttttccgtcg
    22321 ttccgtggtc tacatttgcg aacataatac caatggtgca atggggatca tcgtcaacaa
    22381 gccgctggaa aatctcaaaa ttgaagggat tctggaaaag ctgaagatca cgccggagcc
    22441 gcgtgatgaa tcaatccgtc tggataaacc ggttatgctc ggcggtccgc tggctgaaga
    22501 tcgcgggttt attttgcata ctccgccctc caattttgct tccagcattc gcatttcaga
    22561 caacacagtg atgaccactt cccgcgatgt gctggaaacg ctcggcaccg ataaacaacc
    22621 gtctgacgta ttggtggctc tgggttatgc ctcctgggaa aaaggtcaac tggaacaaga
    22681 aattctcgat aacgcgtggc taaccgcccc ggcggatctg aatattctgt tcaaaacgcc
    22741 gattgccgac cgctggcgcg aggcggcaaa actgattggt gtggatattc tcaccatgcc
    22801 tggtgtggca gggcacgcct gatgagtgga accttactcg ccttcgactt cggcaccaaa
    22861 agcattggtg tagcggtcgg ccaacgcatt accggcactg ctcgcccatt gcctgcaatt
    22921 aaagcacagg acggtacgcc ggactggaac ctcatcgaac gtttactgaa agagtggcag
    22981 ccggacgaaa tcatcgtcgg tttgccgctg aatatggacg gcaccgagca accattgact
    23041 gccagagcgc gtaaattcgc caaccgtatt catggtcgtt tcggtgttga agtaaagctc
    23101 catgacgagc gtcttagcac cgtggaagcc cgttccggtc tgtttgaaca gggcggctat
    23161 cgggcgctca acaaaggcaa agtcgactct gcctctgcgg ttattattct cgaaagctat
    23221 ttcgagcagg gatattaagg cgatttaaaa gcctggcgga tagtcgttac cgccatcttg
    23281 ccggatgcgg cgtgaacgcc ttatccggcc tacatgtggc agcggttgta ggtctgataa
    23341 gacgcgcaag cgtcgcatca gacgtttatt gcctttgtag gcccgataag cttgcacatc
    23401 gggcatggca acgtcacaaa cgcccttccc ccacccgctg ctgataactc tgctgaaacg
    23461 ttatcatccc cacctgctgc ccggtttgaa taacatgcgg taactggtgg gttttccctt
    23521 cgcgaatcaa attccccacc gcgggtgtgt taatcagcaa ttcaaatagc gccacgcgtc
    23581 cttcctgttt atccacttcc agcttttgtg acagcactgc ccgtaaacta cctgccagtt
    23641 gattacgcac ggggtctttt tcctgcgccg gaaatgaatc caccagtcgc tcaactgcct
    23701 gcgcggcacc acgcgtatgt aatgttgcca gcaccaaatg cccggtttct gccgccgtca
    23761 gtgccagacg gattgtctcg ctgtcacgca gctctccgag caaaatcaca tcaggatctt
    23821 cccgcaatgc ggcccgcaat cccgatgcga acgtcataca atgcaaaccg atttcccgct
    23881 gctggatcaa acatcgctgg ctggcataga gatattccac aggatcttcc agcgtcagaa
    23941 tatgcgcatc agcatgttga ttgagataac caaccatcgc cgtcagcgtg gtagatttgc
    24001 cactccccgt cgcccccgtc accagaatca agccattctc gctcttgagt aactccggca
    24061 ataccgttgg tgcgccaagc tgttcgagct gcgggcagtg cgaaggtaac agccgtaacg
    24121 ccagcgaaat gccatgccgt tgcgcgaatg cgctgccacg caatcgctgg ttttccgcca
    24181 gcgacacggc aaaatccagc tggccatttt ccagcaatat tgcccgctga tcgtcatcca
    24241 gccactcccg cagtagctct tcgacgtccg gcgcatcaaa cggcgcagct tccattagcc
    24301 cgcgaatgcg ccatcgtgcg ggccaggcgc tgcacaggtg tagatccgag acgttatgct
    24361 ttacactaag ggccacaatt tcttccatat tcatactaag atcctcggaa aatgaacgat
    24421 attacgcata acctggcaca ggtccgggac aaaatctcag cggctgcaac gcgttgcggc
    24481 cgttctccag aagaaattac gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc
    24541 gcagaagcca ttgatgccgg gcagcgtcaa tttggtgaaa actacgttca ggaaggggta
    24601 gataaaattc gccactttca ggaactgggc gtaacaggat tagaatggca ttttattggc
    24661 ccgttgcagt ctaataaaag ccgcctggtg gcagagcatt tcgactggtg tcataccatc
    24721 gaccgtttgc gcattgctac ccgcctcaac gatcagcgcc cggcagaact tccccctctt
    24781 aacgtactga ttcaaattaa tattagtgat gaaaacagta agtccgggat tcaactggca
    24841 gaactggacg agctggcagc ggcggtcgct gaactaccgc gtttacgtct gcgcgggctg
    24901 atggcaatcc ctgcgcctga gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg
    24961 gctgtagcat ttgccggact gaaaacgcgc tacccgcata tcgacacgct ctctctggga
    25021 atgtcggacg atatggaagc cgccattgcg gcaggtagca cgatggttcg tatcggcact
    25081 gcaatttttg gtgcgcgtga ttactctaaa aaataaggaa ttaaaggaac gccatgaata
    25141 cgttgacttt cctgctttca acggtcattg agctgtatac catggtgctg ttattacgca
    25201 tctggatgca gtgggctcat tgtgattttt acaacccctt ctcacagttt gtagtgaagg
    25261 taacacagcc aattatcggg ccactgcgcc gcgttattcc ggcaatgggt cccattgata
    25321 gcgcctcgct gctggttgcg tacattctca gttttatcaa agccatcgtg ctgtttaaag
    25381 tggtgacctt cctgccaatc atctggattg ccggtttact gattctgctg aaaaccatcg
    25441 gcctgctgat tttctgggtc ctgctggtga tggcgattat gagctgggta agccagggtc
    25501 gtagtccgat tgaatacgtg ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc
    25561 gcctgctacc ggcaatgggt gggattgatt tctcgccgat gatcctcgtt ctgctgctgt
    25621 atgtcatcaa tatgggtgtc gcagaagtat tacaggcaac cggaaacatg ctgctgccgg
    25681 ggctgtggat ggcgttatga gtgccgtaac agttaatgat gacggtctgg ttttacggct
    25741 ctatattcag ccgaaagcca gccgtgattc tattgtcggt ttacatggcg acgaagttaa
    25801 agtcgccatt accgcgccgc cggttgacgg ccaggccaac agccatctgg tgaagtttct
    25861 cggtaagcaa ttccgggtag ccaaaagcca ggtggtaatt gaaaaaggcg aacttggccg
    25921 ccacaaacaa attaaaatca ttaatccgca acaaatcccg ccagaaatcg cggcgttaat
    25981 taattaggta tcctatgcaa aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg
    26041 agctggcgtc gctgcttagc gacttcggtc ttgatatcgt ggcccaaaca gacctcggcg
    26101 tagattccgc tgaagaaacc ggcctgacct ttatcgaaaa cgcgattctg aaagcgcgcc
    26161 atgcggcaaa agtgaccggt ttaccggcaa ttgccgacga ctctggtctg gcggtagatg
    26221 cgctgggtgg cgcgccgggt atttactccg cgcgttattc cggtgaagac gcgaccgatc
    26281 aaaagaatct gcaaaaactg ttggaaacac tgaaagacgt accggacgac caacgtcagg
    26341 cgcgtttcca ctgcgtgctg gtatatctgc gtcacgcgga agatccgact ccgctggtgt
    26401 gccacggtag ctggccgggc gtgattactc gtgaaccagc gggcactggt ggctttggtt
    26461 atgatccaat cttcttcgta ccttccgaag ggaaaaccgc tgccgaactg acccgcgaag
    26521 aaaagagcgc catttcccac cgtggtcagg cattgaaact gctgttggac gctttacgta
    26581 atggttaaat taccgccgct gagtctctac attcacatcc cgtggtgcgt gcagaaatgc
    26641 ccgtactgcg atttcaactc tcacgcgttg aaaggagaag tgccgcacga cgattacgtt
    26701 cagcatctgc tttgcgatct ggacaacgat gtggcttacg ctcagggccg tgaagtaaag
    26761 acaatcttta ttggcggtgg tacgccgagc ctgctttccg gcccggcgat gcaaacgctg
    26821 ctggacggcg tgcgtgcgcg tttgccgctg gcagcggatg cagaaattac tatggaagcg
    26881 aaccctggta cggtagaagc cgatcgcttt gtcgattatc agcgtgctgg tgtgaaccgc
    26941 atctctattg gtgtgcagag ttttagcgaa gacaagctga aacgacttgg gcgtattcat
    27001 ggcccgcaag aagcgaaacg cgcggcaaag ctggcgagcg ggctggggct gcgtagcttt
    27061 aacctcgatt tgatgcatgg actaccggat caatcactgg aagaggcgct tggcgatcta
    27121 cgccaggcca ttgaactgaa tccgccgcat ctttcctggt atcaactgac catcgaacct
    27181 aatacgctgt ttggttcacg cccgccggta ctgccggacg acgacgcgct gtgggatata
    27241 ttcgaacagg ggcatcagtt attaaccgca gcgggttatc agcaatatga aacttccgct
    27301 tacgccaaac ccagttatca gtgccagcac aatctcaact actggcgctt tggcgactac
    27361 atcggtattg gctgcggcgc gcacggcaaa gtgaccttcc cggatgggcg cattctgcgt
    27421 accactaaaa cgcgtcatcc gcgtggtttt atgcaaggaa ggtatctgga aagccagcgt
    27481 gatgtcgaag ccgcagataa gccgtttgag ttctttatga accgctttcg cttgctggag
    27541 gccgcgccac gcgcagagtt tagtgcgtat accgggcttt gcgaagatgt gattcgccca
    27601 cagttagacg aggcgattgc ccagggttat ctcaccgaat gtgcggatta ctggcagata
    27661 acggaacatg ggaagctgtt tttaaattcg ctgctggagc tttttctggc tgagtaaact
    27721 tgtattgccg gatgcggcgt gaacgcc
//
LOCUS       DACAPB010000044        27551 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.044, whole genome shotgun sequence.
ACCESSION   DACAPB010000044 DACAPB010000000
VERSION     DACAPB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.044"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            175..1620
                     /gene="tldD"
                     /locus_tag="IT904_001699"
     CDS             175..1620
                     /gene="tldD"
                     /locus_tag="IT904_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAP3191493.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(1754..2683)
                     /gene="aaeR"
                     /locus_tag="IT904_001700"
     CDS             complement(1754..2683)
                     /gene="aaeR"
                     /locus_tag="IT904_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAP3191494.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            2866..3069
                     /locus_tag="IT904_001701"
     CDS             2866..3069
                     /locus_tag="IT904_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAP3191495.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            3077..4009
                     /gene="aaeA"
                     /locus_tag="IT904_001702"
     CDS             3077..4009
                     /gene="aaeA"
                     /locus_tag="IT904_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAP3191496.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            4015..5982
                     /gene="aaeB"
                     /locus_tag="IT904_001703"
     CDS             4015..5982
                     /gene="aaeB"
                     /locus_tag="IT904_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAP3191497.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVEPAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            6074..6346
                     /locus_tag="IT904_001704"
     CDS             6074..6346
                     /locus_tag="IT904_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191498.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(6402..6665)
                     /gene="yhcN"
                     /locus_tag="IT904_001705"
     CDS             complement(6402..6665)
                     /gene="yhcN"
                     /locus_tag="IT904_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAP3191499.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(7030..7500)
                     /gene="argR"
                     /locus_tag="IT904_001706"
     CDS             complement(7030..7500)
                     /gene="argR"
                     /locus_tag="IT904_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAP3191500.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            7935..8873
                     /gene="mdh"
                     /locus_tag="IT904_001707"
     CDS             7935..8873
                     /gene="mdh"
                     /locus_tag="IT904_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAP3191501.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(8936..10003)
                     /gene="degS"
                     /locus_tag="IT904_001708"
     CDS             complement(8936..10003)
                     /gene="degS"
                     /locus_tag="IT904_001708"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAP3191502.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(10093..11460)
                     /gene="degQ"
                     /locus_tag="IT904_001709"
     CDS             complement(10093..11460)
                     /gene="degQ"
                     /locus_tag="IT904_001709"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAP3191503.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(11614..12012)
                     /locus_tag="IT904_001710"
     CDS             complement(11614..12012)
                     /locus_tag="IT904_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAP3191504.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            12206..13333
                     /locus_tag="IT904_001711"
     CDS             12206..13333
                     /locus_tag="IT904_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAP3191505.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYAIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            13552..13980
                     /gene="rplM"
                     /locus_tag="IT904_001712"
     CDS             13552..13980
                     /gene="rplM"
                     /locus_tag="IT904_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP3191506.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            13996..14388
                     /gene="rpsI"
                     /locus_tag="IT904_001713"
     CDS             13996..14388
                     /gene="rpsI"
                     /locus_tag="IT904_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP3191507.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            14783..15421
                     /gene="sspA"
                     /locus_tag="IT904_001714"
     CDS             14783..15421
                     /gene="sspA"
                     /locus_tag="IT904_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAP3191508.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            15427..15924
                     /gene="sspB"
                     /locus_tag="IT904_001715"
     CDS             15427..15924
                     /gene="sspB"
                     /locus_tag="IT904_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAP3191509.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(15967..17334)
                     /gene="dcuC"
                     /locus_tag="IT904_001716"
     CDS             complement(15967..17334)
                     /gene="dcuC"
                     /locus_tag="IT904_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAP3191510.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            17714..18505
                     /gene="nanR"
                     /locus_tag="IT904_001717"
     CDS             17714..18505
                     /gene="nanR"
                     /locus_tag="IT904_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAP3191511.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            18627..19520
                     /gene="nanA"
                     /locus_tag="IT904_001718"
     CDS             18627..19520
                     /gene="nanA"
                     /locus_tag="IT904_001718"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAP3191512.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            19629..21119
                     /gene="nanT"
                     /locus_tag="IT904_001719"
     CDS             19629..21119
                     /gene="nanT"
                     /locus_tag="IT904_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAP3191513.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIVLVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAVLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            21167..21856
                     /locus_tag="IT904_001720"
     CDS             21167..21856
                     /locus_tag="IT904_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP3191514.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            21853..22728
                     /locus_tag="IT904_001721"
     CDS             21853..22728
                     /locus_tag="IT904_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAP3191515.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            22725..23189
                     /locus_tag="IT904_001722"
     CDS             22725..23189
                     /locus_tag="IT904_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP3191516.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(23265..23570)
                     /locus_tag="IT904_001723"
     CDS             complement(23265..23570)
                     /locus_tag="IT904_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3191517.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(23587..24066)
                     /locus_tag="IT904_001724"
     CDS             complement(23587..24066)
                     /locus_tag="IT904_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAP3191518.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVSSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            complement(24250..25668)
                     /locus_tag="IT904_001725"
     CDS             complement(24250..25668)
                     /locus_tag="IT904_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP3191519.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMHGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(25681..>27551)
                     /gene="gltB"
                     /locus_tag="IT904_001726"
     CDS             complement(25681..>27551)
                     /gene="gltB"
                     /locus_tag="IT904_001726"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAP3191520.1"
                     /translation="IGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIK
                     QVASGRFGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLI
                     SPPPHHDIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIA
                     GYDGGTGASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKA
                     AILGAESFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYF
                     EFIARETRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKA
                     LYCTENNPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTH
                     GDQGLAADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVG
                     SAFRSHEASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTG
                     GIVCILGKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGL
                     ITEHVQHTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
BASE COUNT         6500 a         7013 c         7234 g         6804 t
ORIGIN      
        1 cgtaggtcgg ataaggcgtt tacgccgcat ccgacagccg ttgcctgatg cgacgctgtg
       61 cgtcttatca ggcctacaaa ccggaacata accgtaggct ggatagggcg ttcacgccgc
      121 atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt
      181 cttaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc
      241 gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg
      301 agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc
      361 gatcagggcg ttggtgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac
      421 caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat
      481 agtggtgatg gtaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg
      541 gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat
      601 aaggttgccc gcgaagcgga caagcgcgta caggaagtga ctgccagcct cagtggtgtc
      661 tatgaattaa ttttggttgc ggccaccgac ggcacgctag cggcggatgt ccgtccgctg
      721 gtgcgtcttt ccgtgagcgt tctcgtcgaa gaagatggca aacgcgaacg cggtgccagt
      781 ggcggcggcg gtcgttttgg ttatgaattc ttccttgccg atctcgacgg cgaagtccgt
      841 gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct
      901 gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat
      961 gaagcggttg gtcacggtct ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt
     1021 ggacaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga tggcacgatg
     1081 gtcgatcgcc gaggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg
     1141 ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg
     1201 gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt
     1261 atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt
     1321 gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac ctccggcaaa
     1381 ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac gaagccggtg
     1441 aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc
     1501 aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg
     1561 gttggcgtgg gccagccaac gttgaaagtc gataacctga ctgttggcgg tactgcgtaa
     1621 taatttcact attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc
     1681 ccctttgggg agagggttag ggtgagggga acccgttggc acaggtttga acaacgtaac
     1741 agtacaatat gaattacttc tctttcccgc gcccatgcat ctcctgaaac aatttaccga
     1801 cctcaacaaa ataatccgtc agcgagttga tcacgacctg taccttcagc ggcagtttat
     1861 ctttttcggt atacaacgca taaaccgggc gtggatctga ctggtaacgc ggcagcagga
     1921 tctccagctc cccacgattg atctcgttga tcacccacat cagcggcacg taggcgatcc
     1981 cggcacccgc cgtcagccag cgcaccagcg tcatcggatc attagtcaca aatcttcctt
     2041 gtgggatcag gcgagtcgag atcccttccg gtgcgatcag ttcaaattca ttgtcgggcc
     2101 gcacgctgta ttcaagccat gaatgactac tcaaatcggc gggtttttcc ggtatgccgt
     2161 attgtgtgag atagcttttc gcggcgcaca ccaccatcgg catcgcgccc agacggcggg
     2221 aaaacaggct ggaatcctgc aacgcgccga cgcggattac cacatccaga ccatcggcaa
     2281 tcaggtcggg ggcaggaatt ccggtaacca gattgacgct caaacctggg tattctttca
     2341 gcatttttgc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc
     2401 gcagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt
     2461 catggagcat ccgacggcag ccctggtagt aaattctgcc ggcttcagtc agaccaatgc
     2521 tgcgtgtact acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct
     2581 gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt
     2641 caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa
     2701 aagtgattta gatcacataa tatagataac agcataacag ttacgctaat atattaaata
     2761 tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat
     2821 gctgaaaata ataacgcctg ctctctctat acaaccaagg tcgacatgag tctgtttccc
     2881 gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct tttatcactg
     2941 gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga ctttgtctgg
     3001 catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg
     3061 ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt
     3121 cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc
     3181 cccgtggacg cgtgacgcgc gctttagcgc tgatgtcgtt gcgatcgcgc cggacgtttc
     3241 tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag gacaggtact
     3301 gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc
     3361 ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta atcgtctcgg
     3421 tgtgcaggcg atgtctcgcg aagagatcga ccaggccaac aacgtactac aaacggttct
     3481 gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgcac
     3541 ggtgatccgc gcgccagcgg atggctgggt gaccaacctc aacgtctata ccggtgagtt
     3601 tattactcgt ggatcaacgg cggttgcgct ggtgaaacag aactctttct atgtactggc
     3661 ctatatggaa gaaactaagc tggaaggggt gcgccctggg tatcgtgcag agatcacgcc
     3721 gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgctg gggtcaccaa
     3781 cgccagcagc acgcgtgacg acaaagggat ggcgaccatc gactctaacc ttgaatgggt
     3841 gcgtttggcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag agaacatctg
     3901 gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg acgaaagcca
     3961 ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat cacgatgggt
     4021 attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac cgccattgta
     4081 ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca
     4141 gcggcgattg ttgctgccgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct
     4201 attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg
     4261 gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg
     4321 gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta tgcgtggggg
     4381 ctggccggtt ataccgcgct gatcattgtg atcaccattc agcctgaacc attgcttacg
     4441 ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcaattatg
     4501 gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tggatcgaga gctggaaagt
     4561 ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt
     4621 gtcgataaag cctggggcga cctggttcga cgcaccacgg cgctacaagg tatgcgcagc
     4681 aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat
     4741 acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc
     4801 ccggaattga tcactgatac tttccgcgaa ttttttgaca caccggtaga aaccgcgcag
     4861 gacgtccaca agcagctcaa acgcctgcga agagttatcg cctggaccgg ggaacgggaa
     4921 acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc
     4981 ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg
     5041 gaagtaaaag tagagccagc cgaacgtcat catgcaatgg ttaacttctg gcgaaccaca
     5101 ctttcctgta ttctgggaac gcttttctgg ctgtggacgg gctggacttc cggtagtggt
     5161 gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa tccacgcatg
     5221 gtggcgatcg actttatcta cgggacgctg gccgcgctgc cgttagggct gctctacttt
     5281 ttggtgatta tccctaatac ccaacagagt atgttgctgc tgtgtattag cctggcagtg
     5341 ctgggattct tcctcggtat agaagtacag aaacggcgac tgggctcgat gggggcactg
     5401 gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt cagtcagttt
     5461 ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg
     5521 gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttctgcc
     5581 gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct cccggcactt
     5641 tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa atttcgcctg
     5701 gcgctgacga tgattatcgc ccaccagcgc ctgcgtgatg cgccgatccc ggttaacgag
     5761 gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat atctgcccgt
     5821 agcgatgata aacgtcgtcg gtactttggt cagttgctgg aagaactgga aatctaccag
     5881 gaaaagctac gcatctggca agctccaccg caggtgacgg aaccggttca tcggctggcg
     5941 gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac cgacgccaaa
     6001 agcgtcggtt ttttcatggc tatacttagc gatgcacggc agaactcgcc gcgaaacgtg
     6061 acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga
     6121 tttttatcgt gactttagcc aaacctttgg tctggcgaaa gataaggtac gcgatctcga
     6181 ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt
     6241 tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc
     6301 agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaaagcc
     6361 cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta cagttcagcc
     6421 gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt tgcgcctttc
     6481 tcttccgctt ttttgttcag catttcacgc atatccattg gcgaagacgc cacaccactt
     6541 acggatacgg tcccgattgc ttcacgattt tgtgcttgtg cagcatcaat ggagtcggca
     6601 gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagcaacagt ggttttgatt
     6661 ttcatgattt ttacctcgac ataatctttt agctgggcct ttgtttcgtg acccatatca
     6721 caaaatcaag tatacactaa ttattgagct aattaatacc gcgctaactg tttcagttga
     6781 tactaagtgt taaaaatgtg aattttaata acaaaaaaga attaaaaata cggttattta
     6841 tcgttaattc tagttttttc aattagataa tgaattttgc ataatgtgct ttttttacct
     6901 tatgtattca ttgtgtgaat gatatgtcgc agtaaaacgc actattcgtt aaattaatcg
     6961 caggggaaag cagggggttg agagggataa gcaacatttt ccccgccgtc agaaacgacg
     7021 gggcagagat taaagctcct ggtcgaacag ctctaaaatc gcttcgtaca ggtctttgac
     7081 tgtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg ccagcgatgg tgcccagaat
     7141 accttctgct ttgcccagtg agtccagcag gcgagcaatt aactgcgccg cgccagggct
     7201 ggtatgaatc acgacaactg catcgttgta gtcgatatcc agcaccagat tcttcaatgg
     7261 actggaggtg gttggtacac ccagttcagc tggcaggcag taaaccattt ccattttggc
     7321 attgcgtgta cgtacagcac caaacttggt caacatccgc gagactttag actgattaat
     7381 attgtcaaag ccttgctcct gcaacgcggc gacgatttcg ccctgggagc taaatttctc
     7441 ttctttaagt aatgctttaa atgctttaac tagttcttct tgcttagccg agcttcgcat
     7501 aagtcacccg atatggtggt tgatacaaca ttattgtgca tacagatgaa tttttatgca
     7561 aacagtcagc cctgaagaag gctgaaataa tgttatgaaa gagcgggatt ttatcaaatt
     7621 tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt ctgcttaaaa gtaaattaat
     7681 tgttatcaaa ttgatgttgt tttggctgaa cggtagggta tattgtcacc acctgttgga
     7741 atgttgcgct aatgcataag cgactgttaa ttacgtaagt taggttcctg attacggcaa
     7801 ttaaatgcat aaacgctaaa cttgcgtgac tacacattct tgagatgtgg tcattgtaaa
     7861 cggcaatttt gtggattaag gtcgcggcag cggagcaaca tatcttagtt tatcaatata
     7921 ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc tggcggtatt ggccaggcgc
     7981 ttgcactact gttaaaaacc caactgcctt caggttcaga actctctctg tatgatatcg
     8041 ctccagtgac tcccggtgtg gctgtcgatc tgagccatat ccctactgct gtgaaaatca
     8101 aaggtttttc tggtgaagat gcgactccgg cgctggaagg cgcagatgtc gttcttatct
     8161 ctgcaggcgt agcgcgtaaa ccgggtatgg atcgttccga cctgtttaac gttaacgccg
     8221 gcatcgtgaa aaacctggta cagcaagttg cgaaaacctg cccgaaagcg tgcattggta
     8281 ttatcactaa cccggttaac accacagttg caattgctgc tgaagtgctg aaaaaagccg
     8341 gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct ggatatcatt cgttccaaca
     8401 cctttgttgc ggaactgaaa ggcaaacagc caggcgaagt tgaagtgccg gttattggcg
     8461 gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt tcctggcgtt agttttaccg
     8521 agcaggaagt ggctgatctg accaaacgca tccagaacgc gggtactgaa gtggttgaag
     8581 cgaaggccgg tggcgggtct gcaaccctgt ctatgggcca ggcagctgca cgttttggtc
     8641 tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt cgaatgtgcc tacgttgaag
     8701 gcgacggtca gtacgcccgt ttcttctctc aaccgctgct gctgggtaaa aacggcgtgg
     8761 aagagcgtaa atctatcggt accctgagcg catttgaaca gaacgcgctg gaaggtatgc
     8821 tggatacgct gaagaaagat atcgccctgg gcgaagagtt cgttaataag taattgatta
     8881 gcggataata aaaaaccgga gcacagactc cggttttttg ttttgagcgc acgacttaat
     8941 tggttgccgg atattcctga atggtgacct gcagcgttaa ctgcttatca tcacgcatca
     9001 ctactacagg gatcaccgaa ccagggcgaa tttccgccac ctgatccatc gtctccagag
     9061 cagagatggc cggtttgtta tccaccgaaa taatcagatc gttgacctga atacccgcat
     9121 tcgccgccgg gccgtcaggt gacacttcat taaccacgat cccttgcagt tgatctatac
     9181 caccgccctg cgcgtgcagt ggtgcgatct cacgtccgcc gataccaatg tagccgcgga
     9241 tcacgcgacc atcgcggatc agcttatcca taattttggt tgctaactgg aaaggaatcg
     9301 caaagccgat accttccggc gtttcgccat cgttactctt atcaaacgac agcgtattaa
     9361 tgcccatcag ttcgcccagc gagttcacca gcgcgccgcc agagttaccg tggttaatgg
     9421 aagcatcggt ttggaggaag ttttgccgcc cggtcgggtt cagaccgatt cgacccgtgg
     9481 cactaataat cccctgggta atggtctgcc cgaggttgta cgggttaccg atcgccagta
     9541 ctacgtcgcc aatgtgcggt acgcgacgtg cattaattgg aatggtaggt aaaccgccag
     9601 tggcattaat tttaagtacc gccagatcgg ttagagagtc agatcccacc agcaatgctt
     9661 caaatacacg tccatcctgt aaggcgacga tgatctgatc ggcgtcgttg atgacgtgtt
     9721 tattggtgat gatataaccg cgttgatcca tgattacacc ggatcccagg gtgcggatct
     9781 caagctggtt gtgagagttg gtgttcaaac cacggttgta aacgttaacc accgctggcg
     9841 cggcgcggcg aaccgccaga ttatagctgg caggcgtctc atcggtactg tcaaattgcg
     9901 gagtggaaag cgggttaagg ctgcgcagcg aaggcatggc aaccagcaga atagcgccga
     9961 caattaatcc aatcgcaacg gaacgtaaga gcttcacaaa catgatggag gcgtcattaa
    10021 aaaagggaac ggcagcagca taccacgagt taaccggaca tcacacgtaa gcctgatgcc
    10081 cggtttacga cattaacgca tcagcagata gatgctttca ttgccgcgta caatttgcag
    10141 ggcgatgatg gccggttttg ccgccagcac tttacgcatt tcagcaatcg agttcacccg
    10201 atcgcggttg acgccaatga tcacatcgtc tttttgcaag ccagcctgag cagctgggct
    10261 tcctttgaca acttcatcga ttttaatacc tttgccgcca tcttttagct gaccatcgct
    10321 caacgttgca ccttccagcg ctggcgtgat catttcagcg ctggccgacg aagaggtgct
    10381 ggtatcgagc gtcacttcta cttccagtgg tttgccgtta cgcagcaggc caagcttcac
    10441 tttcgtgccc ggctcggtgg tcgcgatacg agagcgcaac tcagcaaagc tattcagcgg
    10501 tttgccgttg aggctggtaa taatatcgcc cgctttgacg cccgcttttg ccgagccaga
    10561 acctggcaac acttcgctga caaacgcgcc acgctgcacg tcaaggttga aggctttggc
    10621 gatatcggca ctcatctcgg tgcctttgat gcctaacaaa ccgcgtttga tttcaccaaa
    10681 gtcgataagc tgctgcgcca gtgttcgcgc catattactg gggatggcaa atccaatccc
    10741 gacgctcccg ccgccaggcg caaggattgc agtgttgatg ccaattaact caccgttaag
    10801 gtttaatagt gcaccgccgg agttaccgcg gttaatggaa gcatctgtct ggataaagtt
    10861 ttccagacct tcaagattca acccgctgcg gcctaatgcg gaaacaatgc cagaggtggc
    10921 ggtttgccca aggccaaatg ggttaccgac cgctacggca aaatcaccga cgcgcaattt
    10981 atcggagtcg gcaatagcga tttgcgttaa tttgctcggg ttttgaattt gtaacagggc
    11041 gatatcgctc tggtcatcgc taccaatcag ttttgcatca aactcgcgcc catcattgag
    11101 ctgaatactg attttctgtg cctgattaat cacatggttg ttggtcagca catagccttt
    11161 actggcgttg atgatgacac cggagcctaa accttcgaag ggttgtgcag gttgatccgg
    11221 taaatcatca ccaaaaaact ttttgaattc ttccgggatt ttctgtccct gactggccgt
    11281 tccttccacc cgtacgctca ccactgccgg aagcactttt tccagcattg gagccagact
    11341 ggggagaggg gcctgatcgg caacctggcc tggaatcgac gcgacggcct gaaatgacgc
    11401 cgagagagtt aacccgacac ttaacgctaa tgcactcaac agctgggttt gttttttcat
    11461 tattcctgct ctcgtacctg aatgataaga aaagagattc aaaacagttt gatgttattg
    11521 aattttcaga cggctaacaa tgagacacca gattaaatga tagtcgggga gggagagaag
    11581 aggagggcgc aatggctgcg cccgaaaaat aaattagtcg cgcttcgcgc cagtacgcag
    11641 caggccggat gcgccttcag aatagtcgcg aggcatctgc accggtgcct gatcgttgct
    11701 ggcttcagac tctgccagac gattacggaa cgggtttgct tcagcagaca gttccggcag
    11761 caggctgctg gagctttttg ccatgtgctg atacagctgg cgatagtcgt gcgccatggt
    11821 atccagtaat tccgcgctgc gggcaaagtg gctaaccagc tcttcgcgat actcgtccag
    11881 ttcagcttta ttcttttcca gttcgtactg caacgcctgt tgctggcgta gtttacgatt
    11941 accaaaacgc atggccacag caccaataat gatgccgacg actaacccaa ttagcgcata
    12001 ttcccaggtc atgaacatct cccgttgtct tttgtttccg tagggtgttg gcttcaggct
    12061 ccatgcctgc ggctgattat gccactataa ccgttaattc cacagaagtg gaatcccgac
    12121 tgcatatcgc gtagtgtaga acggcctttt tttcgtcaac cgtgaacaac ggcgcaccga
    12181 ttattcaagg aataacaata agatcatgca aagcgttacc ccaacatcgc aatacctgaa
    12241 ggcgctcaat gaaggcagcc atcaacccga cgacgttcaa aaagaggccg tcagccgcct
    12301 ggaaattatt tatcaagaac tcatcaatag caggccacca gcccccagga cgagtgggct
    12361 aatggcgcgg gtcggtaagc tgtggggtaa acgcgaagac acaaagcata cgccagtgcg
    12421 tggcttatat atgtggggcg gtgtaggacg cgggaaaacc tggctgatgg accttttcta
    12481 tcaaagcctg ccgggagagc ggaaacagcg cctgcacttt caccgtttta tgctgcgggt
    12541 gcacgaagag ctaactgcct tacaggggca gaccgatccg ctggaaatta ttgccgatcg
    12601 ctttaaagcc gaaactgacg tgctctgttt tgacgaattt tttgtttctg atattaccga
    12661 cgccatgcta cttggcggtc tgatgaaagc cctgttcacc cgcggtatta ccctggtagc
    12721 gacgtcaaat attccgccgg acgaacttta tcgaaatggc ctgcaacgtg cgcgttttct
    12781 gcctgcaatc gatgccatta aacagcattg tgatgtaatg aacgtggacg ctggtgttga
    12841 ttatcgactg cgtacactca ctcaggcgca tctgtggctt tcgccactca acgatgaaac
    12901 ccgggcgcag atggataaac tatggttggc gctggcgggg gcgaaacgag aaaattcacc
    12961 gacgttagaa atcaaccatc ggccattggc gacaatgggc gtcgagaacc agacgctggc
    13021 ggtctctttt actacgctgt gcgtcgacgc ccgcagtcag catgactata ttgcgctctc
    13081 acgtctcttt catacggtca tgctgtttga tgtaccagtt atgacgcggt tgatggagag
    13141 cgaagcgcgg cgctttattg cgctggtgga tgagttttac gagcgccatg tcaaattagt
    13201 ggtgagtgca gaagtgccgc tgtatgcaat ttatcagggc gagcggctga aatttgagtt
    13261 ccagcgttgc ctgtcacgtc tgcaagagat gcaaagcgaa gagtatctga agcgcgagca
    13321 tttagcaggt taaaacctgc cacaaatcac aaaaaggggt cgatctttga ccccgacttc
    13381 tctataatcc tgcgacccca cgttacaaga aagttttttt cccaaaactt tttgtgtgct
    13441 ggcataggct attcgaaggg gtaggtttgc cggactttgt cgtgtgaacc tcaacaattg
    13501 aagacgtttg ggtgttcacc aacgtgtaac tatttattgg gtaagctttt aatgaaaact
    13561 tttacagcta aaccagaaac cgtaaaacgc gactggtatg ttgttgacgc gaccggtaaa
    13621 actctgggcc gtctggctac tgaactggct cgtcgcctgc gcggtaagca caaagcggaa
    13681 tacactccgc acgtagatac cggtgattac atcatcgttc tgaacgctga caaagttgct
    13741 gtaaccggca acaagcgtac tgacaaagtg tactatcacc acaccggcca catcggtggt
    13801 atcaaacaag cgacctttga agagatgatt gctcgccgtc ctgagcgtgt gattgaaatc
    13861 gcggttaaag gcatgttgcc aaaaggcccg ctgggtcgtg ctatgttccg taaactgaaa
    13921 gtttacgcgg gtaacgagca caaccacgcg gcacagcaac cgcaagttct tgacatctaa
    13981 tcgggattat aggcaatggc tgaaaatcaa tactacggca ctggtcgccg caaaagttcc
    14041 gcagctcgcg ttttcatcaa accgggcaac ggtaaaatcg taatcaacca acgttctctg
    14101 gaacagtact tcggtcgtga aactgcccgc atggtagttc gtcagccgct ggaactggtc
    14161 gacatggttg agaaactgga cctgtacatc accgttaaag gtggtggtat ctctggtcag
    14221 gctggtgcga tccgtcacgg tatcacccgc gctctgatgg aatacgacga gtccctgcgt
    14281 tctgaactgc gtaaagctgg cttcgttact cgtgacgctc gtcaggttga acgtaagaaa
    14341 gtcggtctgc gtaaagcacg tcgtcgtccg cagttctcca aacgttaatt ggcttctgct
    14401 ccggcagaaa acaattttcg aaaaaacccg cttcggcggg tttttttata gctaaaatct
    14461 gaatcagcgt aaaaactgga aagttgcttt ttgctgccac ctgacagaca ggtaaaacaa
    14521 aaaccacatc gccaataagg gactaagtca actatttcag actaaagctc atctcttttt
    14581 ccccatttcc ggcatcgact caccacaatg gtcgcaaaat ctggtaaact atcatccaat
    14641 tttctgccca aatgtcgggt attgctcatt ttttgtttga ttttcgaaca aagagagtcg
    14701 ttccttattg ggtaacacaa cttctgactg gccacctggt ggctggtagc agtaaaaatt
    14761 ctgactatac ctggaggttt tcatggctgt cgctgccaac aaacgttcgg taatgacgct
    14821 gttttccggt cctactgaca tctatagcca tcaggtccgc attgtgctgg ctgagaaagg
    14881 tgtaagtttc gagatcgaac acgtggaaaa ggacaatccg cctcaggatc tgattgacct
    14941 caacccgaat cagagcgttc cgaccctggt ggatcgtgag ctgaccctgt gggaatctcg
    15001 catcattatg gaatatctgg atgagcgttt cccgcatccg ccactgatgc ctgtttaccc
    15061 ggtagctcgc ggtgaaagcc gtctgtacat gcatcgcatc gaaaaagact ggtacacgct
    15121 gatgaacacc atcatcaacg gttcagcttc tgaagcagat gccgcacgta agcaactgcg
    15181 cgaagaactg ctggcgattg cgccggtctt cggtcagaag ccgtacttcc tgagcgatga
    15241 gttcagcctg gtcgattgct atcttgctcc gctgctgtgg cgtctgccgc aactgggcat
    15301 cgagttcagc ggcccgggtg cgaaagagct gaaaggctat atgacccgcg tctttgagcg
    15361 tgactctttc cttgcttctt taactgaagc agaacgtgaa atgcgtctgg gccggagtta
    15421 atctgtatgg atttgtcaca gctaacacca cgtcgtccct atctgctgcg tgcattctat
    15481 gagtggttgc tggataacca gctcacgccg cacctggtgg tggatgtgac gctccctggc
    15541 gtgcaggttc ctatggaata tgcgcgtgac gggcaaatcg tactcaacat tgcgccgcgt
    15601 gctgtcggca atctggaact ggcgaatgat gaggtacgct ttaacgcgcg ctttggtggc
    15661 attccgcgtc aggtttctgt gccgctggct gccgtgctgg ctatctacgc ccgtgaaaat
    15721 ggcgcaggca cgatgtttga gcctgaagct gcctacgatg aagataccag catcatgaat
    15781 gatgaagagg catcggcaga caacgaaacc gttatgtcgg ttattgatgg cgacaagcca
    15841 gatcacgatg atgacactca tcctgacgat gaacctccgc agccaccacg cggtggtcga
    15901 ccggcattac gcgttgtgaa gtaatacaaa aacaggccct ggtggcctgt tttgtctttt
    15961 aatggcttaa taaaatagcg tgattgtggc aatcatattc accacgaaac cgactgccat
    16021 ggggatcgct gttcgcttca ccaccgcaaa aggcgaaacg cctgcaattc ctgacgtaac
    16081 gaccaccacc gcagtaatcg gcgaaaccgc gcgcgctagc gtcgtggcaa aatgcatcgg
    16141 cataatcatt acaaccgctg gtacatgtag tccggctgcg atattcggaa taagactggc
    16201 aaatgacata aacggcgcat tgccagagcc catcacaatg gcacaaatgg caatgaccag
    16261 cgccataata atcatcacgc caataccgcc cagaccagaa tgctccgcac ccctgataac
    16321 cgcatcgaca gtgccaatcg tcgttaagcc tttcgcaaat atttcacccg cgaccaccag
    16381 cgttaccacg ttggcaaact gcgtacccat gccgtcaaaa aacgcctgca catcgtccat
    16441 tgtttcgcgc aagttatgct tgcggaagaa ctcaacaaac atcgtcaccg tcaaactcag
    16501 taacatcacc accaccagat gcagttctga ctgcatcagc ccgacgtggg cgaggaacag
    16561 cgagccgagc atcaggatta acggcatcac aggtaaaatg gcgtaataga gcggcgggac
    16621 attatcgaga gctttttgct ctgcctgttc gtgattgata tttttatctt ttttgtcaaa
    16681 agcgcgttgc acgaaaaagt gggagatcgc caccgagata atgacgcaag aggcgaccgg
    16741 aagctggtag tggaagaagt atgtggcaat tttcattccc gctacctggg cagcaaaaat
    16801 ggagttcgtt tccagaattc cccactcaat ggacattgtg gttgcgataa ccgccaccgc
    16861 agagaggcga cttactccca gactcaccag cgtcggaaat aaggtgacca tcagcaacat
    16921 acccagaccg gaggcgctgg taataaactg cgccatgatt tggccgatga cgtaagttgc
    16981 cgacagaata atatacggcg agcgaatgag ttttaacggg cggcttaaca agctcaccat
    17041 cgcgcgactg gcccctatgc gctccatgta gcgggcataa ccgcccaccg ccataatcga
    17101 cagccccaat ccggcaatgc ggttggacag catgcgcagg atttcattat aaatatcgac
    17161 gatcaagtag ccgctgcttt tggtgggatc gagtacacca ccgaacccta accagacacc
    17221 gcacatcatc aggaagatac ctgcggcagc cagcaccacc tgaggtttgt agtttttcag
    17281 gatcaaatag cccatcgtaa ttaatacgat gacagatata attatgccga acattgttga
    17341 actccgtgtc aaaagaaaac ggtcaatccc ataaacggca gattgaaaac aacgatgtta
    17401 tattttttgc aaggctattt atggtgcgga tgtcgtgttt ttaattgtag gtgaggtgat
    17461 ttttcattaa aaaatatgcg cttatgatta ttttgtaaga acacattcat aatattcata
    17521 atgctcgtga atagtcttat gaataattca aacgggatgt ttttatctgc gttacattaa
    17581 tttttcgcaa tagttaatta ttccgttaat tatggtaatg atgaggcaca aagagaaaac
    17641 cctgccattt tcccctactt tcaatcctgt gataggatgt cactgatgat gttaatcaca
    17701 ctgaccttac agaatgggcc ttatgaacgc atttgattcg caaaccgaag attcttcacc
    17761 tgcaattggt cgcaacttgc gtagccgccc gctggcgcgt aaaaaactct ccgaaatggt
    17821 ggaagaagag ctggaacaga tgatccgccg tcgtgaattt ggcgaaggtg aacaattacc
    17881 gtctgaacgc gaactgatgg cgttctttaa cgtcgggcgt ccttcggtgc gtgaagcgct
    17941 ggcagcgtta aaacgcaaag gtctggtgca aataaacaac ggcgaacgcg ctcgcgtctc
    18001 gcgtccttct gcggacacta tcatcggtga gctttccggc atggcgaaag atttcctttc
    18061 tcatcccggt gggattgccc atttcgaaca attacgtctg ttctttgaat ccagtctggt
    18121 gcgctatgcg gctgaacatg ccaccgatga gcaaatcgat ttgctggcaa aagcactgga
    18181 aatcaacagt cagtcgctgg ataacaacgc ggcattcatt cgttcagacg ttgatttcca
    18241 ccgcgtgctg gcggagatcc ccggtaaccc aatcttcatg gcgatccacg ttgccctgct
    18301 cgactggctt attgccgcac gcccaacggt taccgatcag gcactgcacg aacataacaa
    18361 cgttagttat caacagcata ttgcgatcgt tgatgcgatc cgccgtcatg atcctgacga
    18421 agccgatcgt gcgttgcaat cgcatctcaa cagcgtctct gctacctggc acgctttcgg
    18481 tcagaccacc aacaaaaaga aataatgcca ctttagtgaa gcagatcgca ttatgagctt
    18541 tctgtatggg gtgttgctta attgatctgg tataacaggt ataaaggtat atcgtttatc
    18601 agacaagcat cacttcagag gtatttatgg caacgaattt acgtggcgta atggctgcac
    18661 tcctgactcc ttttgaccaa caacaagcac tggataaagc gagtctgcgt cgcctggttc
    18721 agttcaatat tcagcagggc atcgacggtt tatacgtggg tggttcgacc ggcgaggcct
    18781 ttgtacaaag cctttccgag cgtgaacagg tactggaaat cgtcgccgaa gaggcgaaag
    18841 gtaagattaa actcatcgcc cacgtcggtt gcgtcagcac cgccgaaagc caacaacttg
    18901 cggcatcggc taaacgttat ggcttcgatg ccgtctccgc cgtcacgccg ttctactatc
    18961 ctttcagctt tgaagaacac tgcgatcact atcgggcaat tattgattcg gcggatggtt
    19021 tgccgatggt ggtgtacaac attccagccc tgagtggggt aaaactgacc ctggatcaga
    19081 tcaacacact tgttacattg cctggcgtag gtgcgctgaa acagacctct ggcgatctct
    19141 atcagatgga gcagatccgt cgtgaacatc ctgatcttgt gctctataac ggttacgacg
    19201 aaatcttcgc ctctggtctg ctggcgggcg ctgatggtgg tatcggcagt acctacaaca
    19261 tcatgggctg gcgctatcag gggatcgtta aggcgctgaa agaaggcgat atccagaccg
    19321 cgcagaaact gcaaactgaa tgcaataaag tcattgattt actgatcaaa acgggcgtat
    19381 tccgcggcct gaaaactgtc ctccattata tggatgtcgt ttctgtgccg ctgtgccgca
    19441 aaccgtttgg accggtagat gaaaaatatc tgccagaact gaaggcgctg gcccagcagt
    19501 tgatgcaaga gcgcgggtga gttgtttccc ctcgctcgcc cctaccgggt gaggggaaat
    19561 aaacgcatct gtaccctaca attttcatac caaagcgtgt gggcatcgcc caccgcggga
    19621 gactcacaat gagtactaca acccagaata tcccgtggta tcgccatctc aaccgtgcac
    19681 aatggcgcgc attttccgct gcctggttgg gatatctgct tgacggtttt gatttcgttt
    19741 taatcgtcct ggtactcacc gaagtacaag gtgaattcgg gctgacgacg gtgcaggcgg
    19801 caagtctgat ctctgcagcc tttatctctc gctggttcgg cggcctgatg ctcggcgcta
    19861 tgggtgaccg ctacgggcgt cgtctggcaa tggtcaccag catcgttctc ttctcggccg
    19921 ggacgctggc ctgcggcttt gcgccaggct acatcaccat gtttatcgct cgtctggtca
    19981 tcggcatggg gatggcgggt gaatacggtt ccagcgccac ctatgtcatt gaaagctggc
    20041 caaaacatct gcgtaacaaa gccagtggtt ttttgatttc aggcttctct gtgggggccg
    20101 tcgttgccgc tcaggtctat agcctggtgg ttccggtctg gggctggcgt gcgctgttct
    20161 ttatcggcat tttgccaatc atctttgctc tctggctgcg taaaaacatc ccggaagcgg
    20221 aagactggaa agagaaacac gcaggtaaag caccagtacg cacaatggtg gatattctct
    20281 accgtggtga acatcgcatt gccaatatcg taatgacact ggcggcggct actgcgctgt
    20341 ggttctgctt cgccggtaac ctgcaaaatg ccgcgatcgt cgctgttctt gggctgttat
    20401 gcgccgcaat ctttatcagc tttatggtgc agagtacagg caaacgctgg ccaacgggcg
    20461 taatgctgat ggtggtcgtg ttgtttgctt tcctctactc atggccgatt caggcgctgc
    20521 tgccaacgta tctgaaaacc gatctggctt ataacccgca tactgtagcc aatgtgctgt
    20581 tctttagtgg ctttggcgcg gcggtgggat gctgcgtagg tggcttcctc ggtgactggc
    20641 tgggaacccg caaagcgtac gtttgtagcc tgctggcctc gcagctgctg attattccgg
    20701 tatttgcgat tggcggcgca aacgtctggg tgctcggtct gttactgttc ttccagcaaa
    20761 tgcttggaca agggatcgcc gggatcttac caaaactgat tggcggttat ttcgataccg
    20821 accagcgtgc agcggtcctg ggctttacct acaacgttgg cgcattgggc ggtgcactgg
    20881 ccccaatcat cggcgcgttg atcgctcaac gtctggatct gggtactgcg ctggcatcgc
    20941 tctcgttcag tctgacgttc gtggtgatcc tgctgattgg gctggatatg ccttctcgcg
    21001 ttcagcgttg gttgcgcccg gaagcgttgc gtactcatga cgctatcgac ggtaaaccat
    21061 tcagcggtgc cgtgccgttt ggcagcgcca aaaacgattt agtcaaaacc aaaagttaat
    21121 cctgttgccc ggtctatgta ccgggccttt cgctaaggga agatgtatgt cgttacttgc
    21181 acaactggat caaaaaatcg ctgctaacgg tggcctgatt gtctcctgcc agccggttcc
    21241 ggacagcccg ctcgataaac ccgaaatcgt cgccgccatg gcattagcgg cagaacaggc
    21301 gggcgcggtt gccattcgca ttgaaggtgt ggcaaatctg caagccacgc gtgcggtggt
    21361 gagcgtgcca attatcggga ttgtgaaacg cgatctggag gattctccgg tacgcatcac
    21421 ggcctatatt gaagatgttg atgcgctggc gcaggcgggc gcggacatta tcgccattga
    21481 cggcaccgac cgcccgcgtc cggtgcctgt tgaaacgctg ctggcacgta ttcaccatca
    21541 cggtttactg gcgatgaccg actgctcaac gccggaagac ggcctggcat gccaaaagct
    21601 gggagccgaa attattggca ctacgctttc tggctatacc acgcctgaaa cgccagaaga
    21661 gccggatctg gcgctggtga aaacgttgag cgaagccgga tgtcgggtga ttgccgaagg
    21721 gcgttacaac acgcctgctc aggcggcaga tgcgatgcgc cacggcgcgt gggcggtgac
    21781 ggtcggttct gccattacgc gtcttgaaca catttgtcag tggtacaaca cagcgatgaa
    21841 aaaggcggtg ctatgaccac actggcgatt gatatcggcg gtactaaact tgccgccgcg
    21901 ctgattggcg ctgacgggca gatccgcgat cgtcgtgaac ttcctacgcc agccagccag
    21961 acaccagaag ccttgcgtga tgccttatcc gcattagtct ctccgttgca agctcatgcg
    22021 cagcgggttg ccatcgcttc aaccggaatt attcgcgacg gcagtttgct ggcgctgaat
    22081 ccgcataatc tcggtggatt gctacacttt ccgttagtca aaacgctgga acaacttacc
    22141 aatttgccga ccattgccat taacgacgcg caggccgcag catgggcaga gtatcaggcg
    22201 ctggaaggcg atataaccga tatggtcttt atcaccgttt ccactggcgt cggcggcggt
    22261 gtagtgagcg gcggcaaact gcttaccggc cctggcggtc tggcggggca catcgggcat
    22321 acgcttgccg atccacacgg cccagtctgc ggctgtggac gcacaggttg cgtggaagca
    22381 attgcttctg gtcgcggcat tgcggcggca gcgcagggcg agctggctgg cgcggatgcg
    22441 agaactattt tcacacgcgc cgggcagggc gatgagcagg cgcagcaact gattcatcgc
    22501 tccgcacgta cgcttgcaag gctgatcgct gatattaaag ccacaactga ttgccagtgc
    22561 gtggtggttg gtggcagcgt tggtctggca gaagggtatc tggcgttggt ggaaatgtat
    22621 ctggcgcagg agccagcggc atttcatgtt gatttactgg cggcgcatta ccgccatgat
    22681 gcaggtttac ttggggctgc gctgttggcc cagggagaaa aattatgatg atgggtgaag
    22741 tacagtcatt accgtctgct gggttacatc ctgcgttaca ggacgcgtta acgctggcct
    22801 tagctgccag accgcaagaa aaagcgccgg gtcgttacga attacagggc gacaatattt
    22861 ttatgaatgt catgacgttt aacactcaat cgcccgtcga gaaaaaagcg gaattgcacg
    22921 agcaatacat tgatatccag ctgttattaa acggtgagga acggattctg tttggcatgg
    22981 caggcactgc gcgccaatgt gaagagttcc accatgaaga tgattaccag ctttgcagcg
    23041 ccattgaaaa cgagcaaacc atcaacttaa aaccgggaat gttcgccgtg tttatgccgg
    23101 gagaaccgca taaaccagga tgcgttgtcg gcgagccggg tgagattaaa aaggtggtgg
    23161 tgaaggttaa ggctgattta atggcttaaa tttattagat caggtgtgac ctgacccatc
    23221 tttggcacag agccaaacct gaactgacgg accgctctgt gccattacct gacgttgtat
    23281 tcttttatgt taacaccctt gatactgtat atacctgtcc gcttaattcg cttgcccttt
    23341 ttattcgtaa tagttgctgt aagtgtactg cagcgaatgg aatgacgttc atcaatcacc
    23401 agccgaattg cttctgaagc atcttccacc tctagaaggt cgtgagtctt atttaagccg
    23461 aatggaacaa ggtggaaggc agtttttcct gatgattttc tggttatttt tactggatac
    23521 aacacatcac cacttttatg gattaattca agctgttcaa tgtaaatcat ggcaactttc
    23581 cttcgtttat ttgcatgaat atacaaaccg aaaataggag atgcaataca aaagctgtgg
    23641 aaatatcaaa cagcctctca atttggcaga cagcgtctgc caaatctttg ccgatgggaa
    23701 ttctgcagat ggtgtctgcg gaattcccaa tggtgttaat tcgccagaca ctgtctggcg
    23761 aatttcaaca tgttgaaaaa agaggtaaaa aagcctcatt tggcgtaagt tttttgcaga
    23821 gaacccacgc ttatacctta gacttaaatc ctttgatagt cgcttgatta gctgcgcacc
    23881 atacgcagcc ctggcctcgc caccttgttc aaattcgaca attcgtcggc caatttccca
    23941 gtatgtcgcg gtcatcagcg cgttaacgct acttaccgtt tccgtccggg cgctatcgac
    24001 caggtgaata ataccgtcat ggatttgctg gtagcctgtt gaggttctct ctgagagcga
    24061 ttccattatc tgtcccttta tatggcgctg cgttgaataa tggaatcagc tatacgccgg
    24121 gcatctaccg gtaacaatcc agatgagcgt attacattgg ttttcttcag attatgcgag
    24181 gcgctttcca gaaagaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg
    24241 ggacagagct taaacttcca gccagttcat aataccgtct gccgccttac gaccttcggc
    24301 aatagcggtt accaccagat cggaaccacg gacgatatcg ccgccagcaa agattttcgg
    24361 gttgctggtc tggaaggcgt tgtcgctgcc ttccggggcg atgatgcggc cttgtgaatc
    24421 cagctcgacg ctgtgttttg ccagccattc catgttgtgt ggacggaaac caaacgccat
    24481 gatcaccgca tctgccggaa cgatatgttc ggaacctgca acgatctccg cgcggcgacg
    24541 gcctttggca tccggttcgc ccatttcggt acgtaccatt tttacgccgc tgactttgcc
    24601 gttaccgttc acttcaatcc ccagtggctg gacgttgaat ttgaactcta cgccttcttc
    24661 ccgcgcgttt ttcacttcgc ggcgggaacc cggcatgttc tcttcatcac gacgataggc
    24721 acaggtaacg tgcttcgctc cctggcgcac ggacgtacgc acgcagtcca tcgcagtgtc
    24781 gccaccgcca aggaccacca cgcgtttgcc ttccatgctg acgaacggtt cgtcgcgggt
    24841 ttcaccaaag cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac
    24901 gccatcggcg tcttcgtttt ccagcccgcc gtgcattgac tgataagtcc cgacgccaag
    24961 gaagacggca tcgtaatcac tcagcagatc gtccagctgc acgtcgcggc ccacttcggt
    25021 attgagtttg aattcaatac ccatgccggt gaagatttca cggcgacgcg tcattacctc
    25081 tttttccagc ttgaaggccg gaataccgaa ggtcagcagc ccgccaattt ctggatgacg
    25141 gtcgaagaca acggctttta cgccgttacg cgtcaggaca tccgcacacg ccagacctgc
    25201 cgggcctgcg ccgataatcg ccactttttt accggtctgt ttcacaccag acatatccgg
    25261 acgccagccc atctcgaacg ctttatcgtt gatatagcgc tcaatgttgc cgatggtcac
    25321 cgcgccaaac tcatcgttca gagtgcagga accttcgcac agacggtctt gcgggcagac
    25381 tcgtccgcaa acttccggca gggtgttggt ctggtgcgac agttccgccg cttcaaaaat
    25441 acgcccctcg ttggcgagct tcagccagtt cgggatgtag ttgtgtaccg ggcatttcca
    25501 ctcgcagtat gggttgccgc acgacaggca gcggtcagcc tgcgctttgg cctggccttc
    25561 ggaaaacggc tcgtaaattt caacaaactc aattttgcgg atcttcagcg gtttctttgg
    25621 cggatcaacg cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc
    25681 ttactgcgcc tgcacgcgca actcagctgc gctacgacta cggtgaccca gcagtgcttt
    25741 tacatcactg gacttcggtt taaccagcgc aaatttagtg gcgaaggttg accagttcgc
    25801 cagaatctct tcaccgcgct gagagccggt atgctgcaca tgctcggtga taagaccacg
    25861 cagatgctct tcatggatcg ccagagcgtc aacgcttaag acctcgacca gttccgggtt
    25921 aacgcgtttg cggaaatcgc cgctttcatc gagaacgtaa gcgaaaccgc cggtcatgcc
    25981 cgcaccgaag ttaacgccgg ttttacccag aatgcagacg ataccacccg tcatatattc
    26041 acaaccgttg tcgccaatgc cttctaccac ggtgatagca ccggagttac gcacgccgaa
    26101 acgttcaccc gcgcggcctg cggcatacag acgaccaccg gtcgcgccat acaggcaggt
    26161 gttgccgata atgcttgctt catggctgcg gaaggcggaa ccaaccggag gacgaatggc
    26221 gattaagccg cccgccatgc ctttaccgac atagtcgttg gcatcaccgg tcaggtacag
    26281 ttccacgccg cccgcgttcc acacgccgaa gctctggcct gcggtgccgt tgaagtacgc
    26341 tttgatagga tcggctgcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag
    26401 cgacgcgccg acagaacggt cggtgttgcg aatatcgaac cagaaggttt tgctctggcg
    26461 ctcatcgaca aacggtttcg cctgttgcag caactgcgcg ttcagcaggc cgttatcaaa
    26521 cggcgggttg ttttcggtgc agtagagtgc cttacctgga tgcggttcgg cagtctccag
    26581 cagcttcgac agcgccagct tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag
    26641 gtcggtgcga ccaatcagat ccaccagacg tgttacgcca agctgtgcca tcagctcgcg
    26701 ggtttcacgg gcgataaact caaagtaatt cgtcaccttg aatggcaggc cgtgatagtg
    26761 gttcttacgc agtttgtcat cctgagttgc tacacccgtt gcgcagttgt tcagatggca
    26821 aatacgtaga tatttacagc cgagcgccac catcgggcca gtgccgaagc cgaagctttc
    26881 tgcgccgaga atcgccgcct tgatgatatc gacacccgtt ttcaggccgc catcgacctg
    26941 caaacggatc ttgtgacgca agccgttagc aaccagcgcc tgctgggttt caacaagccc
    27001 cagctcccac ggacagcctg cgtatttcac cgatgaaagc ggactcgcgc cggtgccgcc
    27061 gtcatagcct gcgatggtga tcaagtccgc ataagctttt gccacgccag tcgcgatggt
    27121 gcctactccc ggttcggaaa ccagcttcac ggagatcatc gctttcgggt taacctgctt
    27181 gaggtcgaaa atgagctgcg ctaagtcctc gatagagtag atatcgtggt gcggcggcgg
    27241 ggagatcagc gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gcgtgacttt
    27301 atcacccggc aactgaccgc cttcgcctgg cttcgcgccc tgggcgactt taatctgaat
    27361 gacgtcggca ttgaccagat acgccggagt aaccccaaag cgaccggaag ccacctgctt
    27421 gatgcgcgac actttgttgg tgccgtagcg cgccgggtct tcgccgcctt caccggagtt
    27481 cgaattaccg ccgatgctgt tcatcgcttc cgccagcgcc tcgtgggctt ccgggcttaa
    27541 cgcgccgata g
//
LOCUS       DACAPB010000045        27484 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.045, whole genome shotgun sequence.
ACCESSION   DACAPB010000045 DACAPB010000000
VERSION     DACAPB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.045"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1313
                     /locus_tag="IT904_001727"
     CDS             <1..1313
                     /locus_tag="IT904_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAP3191521.1"
                     /translation="LLLTSYFSKASSESAKPLTNAEWGRFALWLKEKSTTPADLLVSD
                     PKALLQGWHDARISAERIIELLSRGHSLALAMEKWQRAGLWVVTRSDPEYPRRLKQQL
                     KTDSPPVLFGCGNKALLSAGGLAVIGSRNASESDLAFTDQVGAKAAAEGIAIVSGGAR
                     GVDETAMLGAMKQGGVVIGVMADSLLKAATSSKWRKGLMNGHAVLVSPFYPEAGFSAG
                     NAMARNKYIYCLADSSLVIHSGKKGGTLNGAEENLKKGWVPLWVKHTTDKDAANADLV
                     TKGGRWLEADNTQAFKIAELMCNEGGRVNQVKEQMGDLFSMPAQPELFFEPESNPEPD
                     SGPEEVIESEREIVVSESQTKIQAIPEQTAESEAAPSGPVDFYQMFISELQHLAEKPV
                     TVDALIESTQLHKSQLTEWLKRAVDDGVVKKLNRPARFQVKNKK"
     gene            1326..4568
                     /locus_tag="IT904_001728"
     CDS             1326..4568
                     /locus_tag="IT904_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP3191522.1"
                     /translation="MAMTEETLVQEVTADYLLNQLQWDESVLGMYEKLGKEGDLGRLS
                     EQEVVLTRYLGEKLIELNPGLPDVAYQEALRVVCEMPASTNIVAVNKEKYSLHKNGVE
                     VSFHNEKGERVKKRLRLFDFDNYENNHFLLVREFWIKGDIYRRRADLVGFVNGIPLLF
                     MEVKNVHKDIRAAYEQNLADYKDTVPHLFYHNAFIILGNGIDAKIGSVSSKFEHFNDW
                     KRLNESEPGVVDMETLLKGTCSKANLMNIFENFTLFDQSSGRLVKIIARNHQYLGVSR
                     AIDAVINRGERMGKLGVFWHTQGSGKSYSIVFFAQMVHRKLGGNYTFVVLTDREDLDT
                     QIYKTFAGCGLVDNDKDPCRADSGKDLKELIGQQKAFVFTLIQKFNEKVDPKKPYSER
                     DDIIVITDEAHRTQYGTLSLNMRNALPNASFIGFTGTPLFKDDEITKKVFGDYISTYD
                     FQRAVEDKATVPLYYDARGEELVFTDEDGNEHTVADPKGINERIAEKLDELEIEDVDV
                     QQRLERELKRDYHIITATSRLDQIARDFVAHYANAWETGKAMFVCLDKITCVRMHKLI
                     DFYWQQEIKTKEKALLSATDEQDLAWRERQLAWMKETLMAVVVSEEQGEVAKFEKWGL
                     DIKPHRQLLKAGLELADGSRLDMEEAFKSSEHPFRVAVVCAMWLTGFDVPTLSTLYLD
                     KPLKAHTLMQAIARANRVAEGKNNGLIVDYCGILKNLRKALATFAGAGDEGHGGGEGE
                     NEPAKPNEELLESLNESISFVAEFLRKHDFELSRIITETGFAKNAAIAQAKEIINQND
                     ETRKRFEVMAREVFNKFKACINVPGVNGYRDMRDAINVLYKSLQADKEKADISDIMKD
                     LYEIVDGSIDTTHKVKEPKPDGDRIYDISKIDFERLRQEFSRSERKNTTVQSLKHAVE
                     KKLARLMMQNPLRTDYQEHYEKLVKEYNQEKDRVVIEKTFEALLKLNEELSHEEKRAI
                     RECLDEESLVLFDLLSKPNLQPKEIAKIKQVATTLLATLKAERLKVANWQQKESTRDA
                     VKQQIFDFLYDERTGLPVEQYEENEISDITERVFLHIYRAYPELPSPIYG"
     gene            4969..6089
                     /locus_tag="IT904_001729"
     CDS             join(4969..5227,5227..6089)
                     /locus_tag="IT904_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="HAP3191523.1"
                     /translation="MKKRFSDEQIISILREAEAGVPAGELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCD
                     ATGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRR
                     EGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALST
                     GRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTC
                     RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDW
                     RQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
     gene            complement(6399..6680)
                     /locus_tag="IT904_001730"
     CDS             complement(6399..6680)
                     /locus_tag="IT904_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191524.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            7110..8696
                     /gene="pdeC"
                     /locus_tag="IT904_001731"
     CDS             7110..8696
                     /gene="pdeC"
                     /locus_tag="IT904_001731"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAP3191525.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8699..9022)
                     /gene="soxS"
                     /locus_tag="IT904_001732"
     CDS             complement(8699..9022)
                     /gene="soxS"
                     /locus_tag="IT904_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAP3191526.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            9108..9572
                     /gene="soxR"
                     /locus_tag="IT904_001733"
     CDS             9108..9572
                     /gene="soxR"
                     /locus_tag="IT904_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAP3191527.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            10118..11467
                     /locus_tag="IT904_001734"
     CDS             10118..11467
                     /locus_tag="IT904_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP3191528.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGAFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11618..13267
                     /locus_tag="IT904_001735"
     CDS             11618..13267
                     /locus_tag="IT904_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAP3191529.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(13421..14713)
                     /locus_tag="IT904_001736"
     CDS             complement(13421..14713)
                     /locus_tag="IT904_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP3191530.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKGIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSKEKEVSIKVVYFINNVVAHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(14891..16540)
                     /gene="actP"
                     /locus_tag="IT904_001737"
     CDS             complement(14891..16540)
                     /gene="actP"
                     /locus_tag="IT904_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAP3191531.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(16537..16851)
                     /locus_tag="IT904_001738"
     CDS             complement(16537..16851)
                     /locus_tag="IT904_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAP3191532.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(17051..19009)
                     /gene="acs"
                     /locus_tag="IT904_001739"
     CDS             complement(17051..19009)
                     /gene="acs"
                     /locus_tag="IT904_001739"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAP3191533.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCCFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            19402..20838
                     /gene="nrfA"
                     /locus_tag="IT904_001740"
     CDS             19402..20838
                     /gene="nrfA"
                     /locus_tag="IT904_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAP3191534.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            20883..21449
                     /gene="nrfB"
                     /locus_tag="IT904_001741"
     CDS             20883..21449
                     /gene="nrfB"
                     /locus_tag="IT904_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAP3191535.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            21446..22117
                     /gene="nrfC"
                     /locus_tag="IT904_001742"
     CDS             21446..22117
                     /gene="nrfC"
                     /locus_tag="IT904_001742"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAP3191536.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVQACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            22114..23070
                     /gene="nrfD"
                     /locus_tag="IT904_001743"
     CDS             22114..23070
                     /gene="nrfD"
                     /locus_tag="IT904_001743"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAP3191537.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            23090..24808
                     /locus_tag="IT904_001744"
     CDS             23090..24808
                     /locus_tag="IT904_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP3191538.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SAFTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            24801..25184
                     /gene="nrfF"
                     /locus_tag="IT904_001745"
     CDS             24801..25184
                     /gene="nrfF"
                     /locus_tag="IT904_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAP3191539.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            25181..25777
                     /gene="nrfG"
                     /locus_tag="IT904_001746"
     CDS             25181..25777
                     /gene="nrfG"
                     /locus_tag="IT904_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAP3191540.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVSSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     HGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            26119..27432
                     /gene="gltP"
                     /locus_tag="IT904_001747"
     CDS             26119..27432
                     /gene="gltP"
                     /locus_tag="IT904_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAP3191541.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         6541 a         6555 c         7386 g         7002 t
ORIGIN      
        1 ccttgctgtt gaccagctat ttctcaaaag ccagtagcga aagcgcaaag ccgctgacca
       61 atgccgaatg gggtcgcttt gccttgtggt taaaagaaaa gtccaccaca ccagctgatc
      121 tgttggtttc cgacccgaaa gctttgctgc aaggctggca cgatgcgcgt atcagtgcgg
      181 agcgcatcat tgaattgctg agccgtggtc atagcctcgc actggccatg gaaaaatggc
      241 aacgggcagg cctgtgggtg gtcacccgtt ccgacccgga atatccaagg cgtttaaagc
      301 agcagctaaa aacagattcc ccgccagtgc tgtttggttg tggtaacaaa gccttgttga
      361 gtgccggtgg cttggcggtt attggctcgc gcaatgccag tgagtctgat ttggctttta
      421 ccgaccaagt gggggctaaa gcagcggcag agggtattgc cattgtctct ggtggtgctc
      481 ggggtgtcga tgaaacggca atgctggggg ctatgaaaca aggcggtgtg gtgatcggcg
      541 tgatggccga tagcctgcta aaagcagcga ccagcagtaa gtggcgtaaa ggtctgatga
      601 atggccatgc agtgctggta tcgcctttct accccgaggc ggggtttagt gcgggtaatg
      661 ccatggctcg taataaatat atctactgtc tggcagacag ctctctggtg attcactccg
      721 gtaaaaaagg tggcacgctc aacggagcag aagaaaatct gaaaaaggga tgggtgccgc
      781 tgtgggttaa acacacgacg gacaaggatg ccgccaatgc cgatttggtc accaaaggtg
      841 gccgttggct ggaggctgat aatactcaag ccttcaagat tgcggaatta atgtgcaatg
      901 aaggtgggcg tgtgaatcag gtgaaggaac aaatgggcga tctcttttcc atgcctgcac
      961 agcccgaatt gttttttgag cctgaatcta atcctgagcc agattctggg cctgaagaag
     1021 ttatagagtc agagcgcgag atagtcgtta gcgaaagcca gacaaagatt caggctattc
     1081 cagagcagac tgccgaaagc gaagctgccc catcaggccc tgtcgatttc tatcagatgt
     1141 ttatctccga acttcaacac ttggctgaaa agccagtaac cgttgatgcg ctgattgaaa
     1201 gcacacagct tcataagtct cagttaactg agtggctgaa acgtgctgtt gatgacgggg
     1261 tggtgaaaaa gctcaatcgc cctgcacgtt ttcaagtcaa gaacaagaaa tagaaggatt
     1321 tgctcatggc catgacagaa gaaaccctgg ttcaggaagt cactgcagac tacttgctga
     1381 atcagttgca gtgggatgaa tctgtcctgg gtatgtatga aaagctgggc aaggagggtg
     1441 atttaggtcg gctttcagag caagaggtgg tgttaacccg ctatttgggc gagaaactga
     1501 tagagctcaa ccctggcttg ccggatgtcg cctaccaaga ggcgctgcgc gtggtttgtg
     1561 agatgccagc atccaccaat attgttgctg taaataagga gaaatactcc cttcataaga
     1621 atggcgtaga ggtgagcttc cataacgaaa aaggtgaacg ggtaaagaaa cggcttcgcc
     1681 tgtttgattt tgacaactac gaaaacaatc acttcttgct ggtcagagag ttttggatca
     1741 aaggcgatat ttatcgtcgc agagcagacc ttgtgggctt tgtgaatggt attccgctgc
     1801 tgtttatgga ggtgaaaaac gttcacaagg atatccgtgc tgcctatgag caaaatcttg
     1861 cggactacaa agacaccgtc ccgcacctgt tttatcacaa cgcctttatt atcttgggca
     1921 atggtattga cgccaagatt ggctccgttt ccagtaaatt tgagcatttc aatgactgga
     1981 agcgtttgaa tgaaagtgag cctggtgtgg tggatatgga aaccttgttg aaaggtacct
     2041 gctccaaagc caatctcatg aacatttttg agaacttcac tctgtttgac cagagctcgg
     2101 gccgcctggt aaagattatt gcccgcaatc accaatatct tggtgtcagc cgtgccattg
     2161 atgccgtgat taatcgtggc gagcggatgg gtaagttggg agttttctgg catacccagg
     2221 gctctggaaa gtcctattcg attgtctttt tcgctcagat ggtgcatcga aaactgggtg
     2281 ggaactatac ctttgtggtg ctgactgacc gagaagatct cgatacacag atttataaaa
     2341 cctttgcagg ctgtggcttg gtcgacaacg ataaagaccc gtgccgcgcc gatagtggta
     2401 aagacctgaa agagctgatc ggccagcaaa aggcgtttgt atttaccttg attcaaaaat
     2461 ttaacgaaaa agtcgaccct aaaaagcctt attccgagcg cgacgacatt atcgtcatca
     2521 ccgacgaagc tcaccgcacg caatatggca cgttgtcgtt aaacatgcgc aacgccttgc
     2581 ccaatgccag ttttatcggc ttcaccggaa ctccattatt taaagatgat gagataacca
     2641 agaaggtttt tggtgattac atctctacct atgactttca gcgggctgtc gaggataaag
     2701 cgacagtgcc cttgtactac gatgcccgtg gtgaagaatt ggtatttacc gatgaagatg
     2761 gcaacgagca tacggttgcg gaccccaaag gcatcaatga gcgcatagcg gaaaagctgg
     2821 atgagttgga aattgaggat gtagacgttc agcaaaggtt ggagcgagag ctgaagcgtg
     2881 attatcacat tatcactgca accagccgac tggatcagat agcacgtgac tttgtggctc
     2941 attatgccaa cgcttgggaa accggcaagg cgatgtttgt gtgcctcgac aagatcactt
     3001 gtgtacggat gcacaaattg atcgattttt actggcaaca ggaaattaaa accaaagaaa
     3061 aagcactttt gtcggctacg gatgagcagg atctagcttg gcgagagcgg caattggcgt
     3121 ggatgaaaga aacgctgatg gcggttgtgg tcagtgaaga gcaaggtgaa gtcgccaagt
     3181 ttgaaaaatg ggggctggat atcaagcctc accgccagtt gcttaaagca ggacttgaac
     3241 tggctgatgg ctctcgactg gacatggaag aagcctttaa atccagcgaa caccctttta
     3301 gagtcgccgt tgtctgtgcc atgtggctga ccggatttga tgtgcccaca ctttctaccc
     3361 tctatttgga caagcctttg aaggcgcata ccttgatgca agctatcgcc agggctaacc
     3421 gagtagcgga aggcaagaat aacggtttga tcgtggatta ctgcgggata ctgaaaaacc
     3481 tgcgtaaagc attggcaacc ttcgccggtg ctggtgacga gggtcatggc ggaggtgagg
     3541 gcgaaaatga acccgccaag cccaatgaag agttgttaga aagtctgaac gagtcgatca
     3601 gttttgttgc tgagttttta cgaaagcatg attttgaact aagccggatt attacagaaa
     3661 caggttttgc taaaaatgca gcgattgctc aagccaagga aatcatcaat caaaatgacg
     3721 aaacacggaa acgtttcgag gtaatggcca gagaggtgtt caacaagttt aaagcctgca
     3781 ttaacgtgcc gggcgttaat ggctatcgtg atatgcgcga tgccatcaac gtactctata
     3841 agagtttgca ggcagataaa gagaaagccg acatctccga tattatgaag gatctgtacg
     3901 agattgttga cggtagcatc gataccaccc ataaagtcaa agaaccgaaa ccggatggtg
     3961 atcgaattta cgatatcagc aagatcgatt ttgaacggct ccgccaggag ttttctagat
     4021 cagagcgaaa gaatactaca gtgcaatcgc tcaagcatgc ggttgagaag aagctagcca
     4081 ggctcatgat gcagaacccg ttacgtaccg attatcaaga gcactacgaa aaattggtta
     4141 aggaatataa ccaagaaaag gatcgcgttg ttattgaaaa aacctttgag gcactgttaa
     4201 aactgaatga agagctttct catgaagaga agcgcgcaat cagggaatgc ttggacgagg
     4261 agtctttggt attgtttgat ctccttagta agccgaattt gcagccaaaa gagattgcca
     4321 agatcaaaca agtcgccact acgttgttgg caacgctaaa ggcagagcgg ctgaaagttg
     4381 caaactggca gcagaaagaa tcgacgcgcg acgcggttaa gcagcagata tttgattttc
     4441 tttatgatga aagaacaggg ttacccgttg agcaatatga agaaaatgag atttcagata
     4501 tcactgaaag ggtgtttctt cacatatatc gggcgtaccc cgaactgcca tcgcccattt
     4561 atgggtgaag aattacgcgg agttacgcat ggtttgcgta attacgcata tgatgcataa
     4621 ttgtgcgtaa tttgaggcaa ctctatccca aacctcaaat aggccggtta cctgccggat
     4681 ggtgccttgg tctaatgagc tcttagccgg tgacggcttg aaagcgggtg taaataccaa
     4741 ggtttgacat agagttgatg gcaagagcgg gaaatggatt ttatttcgcc gagatcttgc
     4801 taaaatgcga ttcaatgttc ggtagaaaca accagccttc caatacccgc ctttttgatt
     4861 ttgggtgttg gagtcgcctg agttgatcag gtgctgttga cctgctcccc gttgattagt
     4921 acaccccgat gttagtaatg tcttcataag ccacatgagg acatccccat gaagaagcgt
     4981 ttttccgacg aacagatcat cagtattctc cgcgaagccg aagctggggt acccgccggt
     5041 gaactctgcc gcaagcatgc catttccgat gccacgtttt acacctggcg taagaagtat
     5101 ggcggtatgg aggtgcctga agttaagcgc ctgaagtcgc ttgaggaaga gaacaccaga
     5161 ctcaagaagc tgcttgccga agccatgctg gataaagagg cgcttcaggt ggctcttggg
     5221 cgaaagtact gacgacagac cagaagcggg aagccgtgat gttgatgtgt gatgcgaccg
     5281 gtctgtcgca acgtcgtgcc tgcaggctta caggtttatc cctgtcgacc tgccgctatg
     5341 aggctcaccg tccggctgct gatgcgcatt tatcagggcg catcactgag ctggcactgg
     5401 agcgcaggcg ttttggctac cgtcgtattt ggcagttgct gcgccgtgaa gggcttcatg
     5461 ttaatcataa gcgcgtgtac cggctttatc acctcagtgg tctgggcgta aaacgcagaa
     5521 gacgtcgtaa agggctggca acagaacgtc tgccgctgct ccgtccggcg gcgcccaatc
     5581 tgacctggtc gatggatttc gtcatggacg cactttccac cggtcgcagg atcaagtgtc
     5641 ttacctgcgt cgatgatttc acaaaggaat gcctgacggt cactgttgcc tttgggattt
     5701 caggcgttca ggtcacgcgt attctggaca gcattgcact gtttcgaggc tatccggcga
     5761 cgataagaac tgaccagggg ccggagttca cttgccgtgc actggatcaa tgggcctttg
     5821 agcatggtgt tgagttgcgc ttaatccagc cgggcaagcc aacgcagaac ggatttattg
     5881 agagctttaa cggacgattt cgcgatgaat gtttgaatga gcactggttc agcgatatcg
     5941 ttcatgccag gaaaattatt aatgactggc ggcaggatta taacgaatgc cgcccgcact
     6001 ccacgctgaa ttatcagaca ccgtctgaat ttgcagcggg ctggagaaag ggtcattctg
     6061 agaatgaaga ttccgacgtt actaactgag tgttgtatct aatcgtgggg gcaggtcact
     6121 gtcgctgaaa gtgtcaagat tctatcttta cgcttggcgg ctaatacttc cacagtacct
     6181 gcccaggaag ttgacaatat tgggcttaac ttaacagctt tggctttctt tatgataaaa
     6241 tccaatattt tcaatgggtt gaatggtttg tttgctgctt tatgtgaact accgttctga
     6301 tttgtcatta aaacaatagg ttatattgtt ttaaggtgga tgattaaagc atctgccagc
     6361 cataaaaaag aagcctccgt tatggaggct tctacgtatc aggtcaaaat cattggccat
     6421 tgtggcggtg tctgattcat ctcttctatc atcaccgact cgatgttatg ccagatagcg
     6481 gtgatgtccg ccattgtgat gccaagcaac cccattgcga accaacaggc ggaaacaacg
     6541 cccagaccgc tgctgatcac cgcaagcccg acataatcag accgacgaaa acggatgttg
     6601 agtgtgctgg ccagaaacat cattacggca ctaagaagtt gccagcgaag aagaaccacg
     6661 ccagtggtga gggtagccat caaacaattc ctctgaaaag agccgatgcc ctggacagcg
     6721 cggcgttgtt cacgggaggc gtggtacact ctggctatcg cggggcttgc agaacacaaa
     6781 aatgaaacac tctgtttgtt tcattaattt tgtgaactat atcacaattg attgtttgtt
     6841 agccatatta ggccgtgact tttattgctg tacagattat gtggtttttc agtggcatta
     6901 agggcatatc ttcccgccgc ctctgcattc ctgtaggaaa ttaattttga atatcaatga
     6961 attattttca tccaggtgac gattagaaag gtatcaattt caaatcaggc aaaagtgcta
     7021 tttataccgt aagatttatc taaagacgtc ggtacccagg gttttcacct tacaatggcc
     7081 gggtataaac aggcaggaaa ttgatagcaa tgagtcatcg tgcacgacac caattactgg
     7141 cgttgccggg cattatcttt ttagttctct ttcccatcat tctttcgcta tggattgcct
     7201 tcctttgggc aaaatcagaa gtgaataatc agctccgaac ctttgctcaa ctggcactgg
     7261 ataaatccga gctggtcatt cgccaggcag atttagtgag cgatgcagct gaacgctatc
     7321 aggggcaagt ttgcactcca gcccatcaaa agcgaatgtt gaatattatt cgtggctatc
     7381 tttatattaa tgaattgatc tatgcccgtg ataaccattt tttatgctca tcgctgatag
     7441 cgcctgtaaa cggctatacg attgcaccgg ccgattataa gcgtgaacct aacgtttcta
     7501 tctattatta ccgcgatacg ccttttttct ctggctataa aatgacctat atgcagcggg
     7561 gaaattatgt ggcagttatc aaccctctct tctggagtga agtgatgtct gatgatccga
     7621 cattgcaatg gggtgtgtat gatacggtga cgaaaacctt tttctcgtta agcaaagagg
     7681 cctcggcagc aacgttttct ccgctgattc atttgaagga tttaaccgta caaagaaatg
     7741 gctatttata tgcgacagtt tattcgacaa aacgcccaat tgcagccatt gttgcgactt
     7801 catatcaacg tcttataacc catttttata atcatcttat ttttgcgttg cccgccggta
     7861 ttttggggag tcttgttctg ctattactct ggctacgtat tcgacaaaac tatttatctc
     7921 ccaaacgtaa attgcaacgc gccctcgaaa aacatcaact ttgtctttat taccagccaa
     7981 taatcgatat caaaacagaa aaatgtatcg gcgctgaagc gttgttacgt tggcctggtg
     8041 agcaggggca aataatgaat ccggcagagt ttattccgct ggcagaaaag gaggggatga
     8101 tagaacagat aactgattat gttattgata atgtcttccg cgatctgggc gcgtacttgg
     8161 caacacatgc cgatcgctat gtttctatta acctgtcggc ctccgatttt catacgtcac
     8221 ggttgatagc gcgaatcaat cagaaaacag agcaatacgc ggtgcgtccg cagcaaatta
     8281 aatttgaagt gactgagcat gcatttcttg atgtcgaaaa aatgacgccg attattctgg
     8341 ctttccgcca ggctggttac gaagtggcaa ttgatgattt tggtattggc tactctaact
     8401 tgcataacct taaatcattg aatgtcgata ttttgaaaat cgataaatcg tttgttgaaa
     8461 cgctgaccac ccacaaaacc agtcatttga ttgcggaaca catcatcgag ctggcgcaca
     8521 gcctggggtt aaaaacgatc gctgaaggcg tcgaaactga ggagcaggtt aactggctgc
     8581 gcaaacgcgg cgtgcgctat tgccagggat ggttttttgc gaaggcgatg ccgccgcagg
     8641 tgtttatgca atggatggag caattacccg cgcgggagtt aacgcgcggg caataaaact
     8701 acaggcggtg gcgataatcg ctgggagtgc gatcaaactg ccgacggaaa acgcgggaga
     8761 aggtctgctg cgagacataa cccaggtcca ttgcgatatc aaaaatcgga cgctcggtgg
     8821 tgcgcaactc aacggcggcc agtaacaggc gacgttggcg aatgtaatcg ccaagcgtct
     8881 gatgcgtcac cgtgcggaac attcgttgca agtaccactt tgaatagcct gatttttttg
     8941 cgactacatc aatgttaagc ggctggtcaa tatgctcgtc aatccatgcg ataagatcct
     9001 gaataatttt ctgatgggac ataaatctgc ctcttttcag tgttcagttc gttaattcat
     9061 ctgttgggga gtataattcc tcaagttaac ttgaggtaaa gcgatttatg gaaaagaaat
     9121 taccccgcat taaagcgctg ctaacccccg gcgaagtggc gaaacgcagc ggtgtggcgg
     9181 tatcggcgct gcatttctat gaaagtaaag ggttgattac cagtatccgt aacagcggca
     9241 atcagcggcg atataaacgt gatgtgttgc gatatgttgc aattatcaaa attgctcagc
     9301 gtattggcat tccgctggcg accattggtg aagcgtttgg cgtgttgccc gaagggcata
     9361 cgttaagtgc gaaagagtgg aaacagcttt cgtcccaatg gcgagaagag ttggatcggc
     9421 gcattcatac cttagtggcg ctgcgtgacg aactggacgg atgtattggt tgtggctgcc
     9481 tttcgcgcag tgattgcccg ttgcgtaacc cgggcgaccg cttaggagaa gaaggtaccg
     9541 gcgcacgctt gctggaagat gaacaaaact aaagcgccac aagggcgctt tagtttgttt
     9601 tccggtcttt gtctttctct ctatcccgct ggtacacagg agggtttccc ccgacgtcaa
     9661 cacaccccat tcgagcacgt ggtggaggtt ccggttggtg ttgatgcttt aattgtatgt
     9721 caccggcatt tcttcgccag tgtaaaagta tactttttat ccgcaatatt tttgtcatct
     9781 cagacgattt tttatcgcaa tcctgaacgg tatacggctc gataacgctg caatcttgcg
     9841 caccgacgat aacgtttgcg catcaattgc ctggtttttc atcgtcaaga caataaaaga
     9901 gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat cgccggataa tcgtttgctt
     9961 tttttaccac ccgttttgta tgcgcggagc taaacgtttg cttttttgcg acgcagtaaa
    10021 ttgtcgcaaa cctggagcag gaagataacg tttcgctggc aggggattgt ccgccacgca
    10081 tcttgacgaa aattaaactc tcaggggatg ttttcttatg tctacgccat cagcgcgtac
    10141 cggcggttca ctcgacgcct ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga
    10201 agtggttgcc gggttaacaa cgtttctggc gatggtctac tcggtcatcg tcgttccagg
    10261 tatgttgggt aaagcaggct tcccgcctgc ggcagttttc gttgcaacct gtctggttgc
    10321 cggactcggt tctatcgtga tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc
    10381 catctccctg acggcattta ccgcattcag cctggtgctg gggcaacata ttagcgtacc
    10441 tgtcgcgctg ggtgccgtgt tcctgatggg tgtgctgttt acggtaattt ctgccacggg
    10501 tatccgtagc tggattttgc gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg
    10561 tatcggtctg ttcttgctgc tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc
    10621 gcttgatggt ctgcccgttg cgctgggtga tttcgcgacc ttcccggtga ttatgtcact
    10681 ggtaggtctg gcggtgatca tcggcctgga aaaactgaaa gtccctggtg gcattctgct
    10741 gaccattatc ggtatctcaa ttgtcggttt gatcttcgat cctaacgtcc atttctccgg
    10801 cgctttcgcc atgccttcat tgagcgatga aaacggcaat tcactgattg gcagcctgga
    10861 cattatgggc gcgctgaatc ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc
    10921 agtatttgat gccaccggaa ctatccgtgc cgtcgccggc caggcgaacc tgctggataa
    10981 agatgggcag atcatcgacg gtgggaaagc actgaccact gactccatga gcagcgtttt
    11041 ctctggcctg gtgggtgcgg ctccggcagc ggtatacatc gagtctgcgg cgggtacggc
    11101 ggcgggcggt aaaaccggtt tgacggctat caccgttggc gtgctgttcc tcctgattct
    11161 gttcctctct ccgctctctt acctcgttcc ggggtatgca acggctccgg cgctgatgta
    11221 cgttggcctg ctgatgctga gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc
    11281 gatggcgggt ctggttacgg ctgtattcat cgtgctgacc tgtaacatcg taacaggcat
    11341 catgatcggc ttcgcgactc tggtgattgg tcgtctggtt tccggcgaat ggcgcaagtt
    11401 gaacatcggt acggtcgtta tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc
    11461 tatctaatct ttcatcggat tctgaaaacg ggtggcaatg gctgcccgtt tttattttct
    11521 ccgcacattg tgtgagcttt tgcgatattc tgaaaaaatg agaattcagg cataacgtct
    11581 ttctcagatc ataaaaaaca tcgcaaacag ggaacgcatg gaaatcttct tcaccatact
    11641 gataatgacc ctcgtggtct cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat
    11701 cccgcttccg cttatgcaaa tcgccatcgg tgcgctactg gcgtggccga cgtttggttt
    11761 gcatgtggag tttgatcctg aactcttttt agtcttgttt atcccgccgt tgctgttcgc
    11821 tgatggctgg aaaacgccga cccgtgaatt tctcgaacat ggtcgagaga tttttggcct
    11881 cgcgctggcg ctggtggtgg tcaccgtggt cggcattggc ttccttattt actgggtggt
    11941 gccgggcatt ccgctgatcc ccgcctttgc gctggcggcg gtactttctc cgaccgatgc
    12001 tgtggcgctc tccgggattg ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt
    12061 gcagggcgaa gcgttgatga atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc
    12121 agtggcgatg gggacgatga tcttcaccgt cggcggtgcg acggtcgaat ttatgaaagt
    12181 agccattggc ggtattcttg ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg
    12241 attcctcagc cgctggggcg gtgatgaacc cgcgacgcag attgttctgc tgttcttgct
    12301 gccattcgct tcttatctga ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt
    12361 tgccgccggg atgaccatca cccgctccgg tgtgatgcgc cgtgcgccgc tggcaatgcg
    12421 cctgcgcgca aacagcacct gggcgatgct ggaatttgtc tttaacggca tggtgttcct
    12481 gctgttaggt ctgcagctgc cgggtattct ggagacgtcg ctgatggcgg cagaaatcga
    12541 ccctaacgtc gaaatctgga tgctgtttac cgatattatt ctgatatatg cggcgctgat
    12601 gctggtccgt ttcggctggc tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa
    12661 gaagccgatg gagtttggtt cgtggaccac acgagaaatc ctgatcgcgt ctttcgccgg
    12721 ggtgcgtggg gcgatcactc tggccggtgt gctctctatc ccgctgctct tgccggatgg
    12781 taacgtcttc ccggcgcgct atgagctggt gttcctggcg gctggcgtca ttctcttctc
    12841 gctgtttgtc ggcgtggtga tgttgcctat tctgctacaa cacattgaag tcgcggacca
    12901 ttcgcaacaa ttgaaagagg aacgtattgc gcgagcggca acggcagaag tggcgattgt
    12961 ggcgatccag aaaatggagg agcgtctggc ggcggatacc gaagagaata tcgataacca
    13021 gctgcttacg gaggtcagtt ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg
    13081 taacgacgtt gaaagttccg tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc
    13141 attgcgttct gaacgtgctg aactttacca cctgcgcgcc acgcgggaga tcagcaacga
    13201 aacgctgcaa aaattactgc acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa
    13261 tcagtaagcc gaaagcgacg taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt
    13321 gcacctttaa caggttagct cgggctaaca ttcatgattc taaaacaaat aagttatctc
    13381 tcttgtaaag tcaattcatt ggctgttctt cgacttaacc tcaatctgcg tgttgcataa
    13441 tatctgcagt aatcttcata gcgactgcat taatattatc ctcagtttgt gacatgccag
    13501 tatgagaggt acaatcttct tcaaagaatt tctgtagtgt taccagggta tcactatcaa
    13561 tggggatatt ttcctcccca cggagaatgg aaatgcccat tccctggacg ctagcctcat
    13621 caaaattaac ccgatagaga cgggtattaa acatacaggc ttttgttaaa atagaacagt
    13681 tgaaatggca gttgcttaaa ttggcattat taaaattaac attagccata ttagagccgg
    13741 tacatataac catttcgagg aatgtatttg tcagccacgt acaaaagagt ttgctatcaa
    13801 ttaagtttat attctggagg attgctttgt tcagattgac gcgagacaga tcagcatgtt
    13861 caaagttaat taaatcgagt ttagagaaac taaggtcggc tttaaataaa ttaacatatg
    13921 gcgctattac tttataaaga tttacctcag ccatgtacgc catataaaaa tttgaatagt
    13981 caaggaaggt atttttaata ttggctctat tcattgctgc attgtagaat tgaacatgca
    14041 tagcggagca tgcatttaga gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat
    14101 cgagaataca attatcaaaa agtatttttt ccattatggc gtaataaaaa ttcgttttat
    14161 acatattaca gtcctggaaa atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg
    14221 ataaatcttc atctttaatc acgatacctt tcaggctaag gtgtgaaaaa tcgtaaccac
    14281 catttactgt ctgtggaatt tcgatagtat tattatgggc gacgacatta tttataaaat
    14341 ataccacctt aatggatact tccttttctt tactaaggta aactcttctc gtaattctat
    14401 cgtcacattt attatttgca tataaagcta tatctataaa atgtttatgt attttcctgt
    14461 ccgtttcctt cgtgattttt cctgatggta aactatcata aattcctttc attttctgaa
    14521 tcaaacctat cactttttca tgatcatcca ttttgaaaag agagagggct ttattgtcaa
    14581 ttaacgtgtt tttatgatgt ttgctgtaat tatatgattt tgttttcttt gtatgtgatg
    14641 aacttgtgaa agagtggtta tcgttaatct ggcaaagaga gaaaaacata ttatttaaac
    14701 cattatagcg cataaaatat ccttataatt aacaagaaag aaaaggcata ttctctgcat
    14761 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
    14821 tatgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
    14881 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
    14941 actgtgcacg gaacagttca cgctcgcgcg caccttccgc tgagttatcg gttgccgaga
    15001 agaaccagat gccgaggaat gccacgctga tagagaacag cgccgggtat tcatacggga
    15061 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
    15121 ccactgcggt aatcagcccc agccagccac ccatcatcgc gccacgagtg gtcagtttcg
    15181 accagtacat agaaagcaga atgatcggga agttacagct cgctgcgatg gcaaacgcca
    15241 ggcctaccat gaaggcgata ttctgattct caaacagcac gccgaggata atggcaatca
    15301 cacctaaaat cagcacggtg attttcgata cccgcagttc ttcacgttcg gtcgcgcctt
    15361 ttttgaagac gttagcgtac aggtcatgcg aaaccgctga tgcgcccgcc agcgtcagac
    15421 cagcaaccac cgcaagaatg gtggcaaaag caaccgctga aataaaaccg aggaacaggt
    15481 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
    15541 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
    15601 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
    15661 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
    15721 caaacatcag tcccagaccc agagagagcg cggagatcgg atctttcacc agcccgcccg
    15781 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
    15841 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
    15901 gcacggcttt gataatttgc acccaggtgg tcgccagcat gccgccgaac aggacgtaca
    15961 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
    16021 cgatcagttt acctgcgcct accatctggg cgataaggta aagcgccacc accaccagag
    16081 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag gcggtaagag gccacatcgg
    16141 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
    16201 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
    16261 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
    16321 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgcgttttg
    16381 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
    16441 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
    16501 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
    16561 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
    16621 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
    16681 cggattcagc ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat
    16741 ataaactgcc agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa
    16801 ctccctgaaa tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc
    16861 tccagaggta atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct
    16921 cgggtaaccc ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat
    16981 gcctgatgcg acgctgtcgc gtcttatcag gcctacaaac cgttaccgac tcgcatcggg
    17041 caattgtggg ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacta
    17101 cgccaggatc ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt
    17161 tgcgcagaat acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt
    17221 gcagcacgtc tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg
    17281 cgtacagttc tggtgacggt tcctccccgt gattaagcgt gacgtaggcg tagatcgcct
    17341 gacctttaat attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca
    17401 ccagcgccga ctcaatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt
    17461 ccacacgccc ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga
    17521 aatacatatt tttgaaggtg gagaagtagg tctgttcaaa acgttcgtga tcgccaaaca
    17581 gcgtacgcgc ctgacccggc caggagtcgg tgattaccag gctaccttcg gtggccccct
    17641 ccagcgggtt accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg
    17701 ccgaaccggc tttcagctcg gtagcgccag gcagcggggt gatcatgaaa ccgccggttt
    17761 cggtctgcca ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc
    17821 actcccacgc ttccgggtta attggctcgc ccacggaacc gagaatgcgc agcgacgaac
    17881 ggtcggtgcc ttcgatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg
    17941 cggtatagag aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg
    18001 gccagttcgg tacgccttca aacatcagcg tggtcgcacc gcaggccagc gggccgtaca
    18061 gcaagtaact gtgtccggtc acccagccca catcggcggt gcaccagtag atatcacccg
    18121 gatgataatc aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag
    18181 tatgcagcac accttttggc ttaccggtag aaccggaggt gtagagaata aacagcggat
    18241 cttcggcgtt catctcttcc gcctggtgct gatcgctcgc ttgctcaacc aggtcgtgcc
    18301 accacaggtc gcgcccttcc tgccagtcaa ttttcccgcc agtacgcttc agtaccacca
    18361 catgctctac gctggtgacg ttcgggtttt tcagcgcgtc atcaacgttt ttcttcagcg
    18421 gaatactgcg cccggcacgc acaccttcgt cggaagtgat caccagtcgt gagttggaat
    18481 caataatgcg cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg
    18541 cgccaatgcg ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa
    18601 tcgccaccac atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcagcaga
    18661 cgtcgcggtg cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc
    18721 agatgatggc ggtacgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca
    18781 gattcagcgt gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtgt
    18841 ttttcacctt ctggtaaggt ttgatccagt cgagaatttt tccctgttcg ccccagaagg
    18901 tatcaggtac gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc
    18961 aacggtctgc gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct
    19021 ccttgtagga tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt
    19081 gtttagtatt tgggcgacag atcacgcaaa agtagaattg tgcaaataaa cggcagggta
    19141 atttttgaag gtcagcaaca aaagttgatt aattctttga ggaacatgca gttatgcatg
    19201 ctgttgaaaa agaggaagat actgactaac tctaaagtgg tattttacat gcacttacaa
    19261 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcgatgtca
    19321 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg
    19381 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
    19441 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
    19501 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
    19561 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
    19621 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
    19681 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
    19741 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
    19801 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
    19861 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
    19921 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
    19981 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
    20041 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
    20101 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
    20161 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
    20221 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
    20281 gaaggcaaac tctacaccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
    20341 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
    20401 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
    20461 gcgaaagcgg cgctggatgc aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat
    20521 atccgtcatg cccagtggcg ttgggatctg gcgatcgctt cccacggcat tcatatgcac
    20581 gcaccggaag aaggtctgag gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc
    20641 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
    20701 atctcgacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
    20761 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaat
    20821 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
    20881 atatgagcgt attacgttcg ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga
    20941 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tggtgatcgc tacgaagtta
    21001 cccagcagcg taacccggat gccgcctgtc tggactgtca taagccagat accgaaggta
    21061 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
    21121 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
    21181 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgc catctgccgg
    21241 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
    21301 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
    21361 ttaagatttg cgtcgattgc catagcgatc agcgcaccaa tccgaacttt aacccggcgt
    21421 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
    21481 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
    21541 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
    21601 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
    21661 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
    21721 ccagcactgc gatcatgcgc cgtgcgttga tgtctgcccg accggtgcgt cttttcgcga
    21781 tgctgccagt gggattgtcg atgttaaccc ggatctctgc gtcggttgtc aatactgcat
    21841 cgccgcttgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
    21901 cgatttctgc cgtaagacca atttgcaggc cggtaaactg cccgcgtgcg ttcaggcttg
    21961 cccgaccaag gcgctgacgt ttggcaatct ggacgatccc aacagtgaga tttcgcaact
    22021 gctgcgccag aagcccactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
    22081 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
    22141 gaatcactgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
    22201 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
    22261 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
    22321 atcctcgtct ttcacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
    22381 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tttacatggt ggtgctggtg
    22441 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
    22501 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
    22561 acattgatgc tggtgctggc ggtgttgttg ggggcttata ccggttttct gctgtcggcg
    22621 ctgaaatcgt atccgttcct taataacccg atcctgccgg tgctgttcct cttctccggc
    22681 atctcgtccg gtgcggcggt ggcactgatc gccatggcga tacgccaacg cagtaacccg
    22741 cattccacgg aagcgcagtt tgtacaccgt atggaaatcc ccgtggtatg gggtgaaatc
    22801 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agtgcgtgcg
    22861 ctggtggcgg cattgggcgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
    22921 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
    22981 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgttgcg atttttcatt
    23041 ctctacgccg ggcagttaac ggtggcgtaa gccagaacag aggtggtttc tggacgtatt
    23101 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
    23161 gttgacggcc tttgcgggag tgcggttacg ctggcctgcc atgatgcgac tcacttgcat
    23221 cggcattctg gcacagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
    23281 tctcatcagc gatttctcgg tcatttatgt cgcccaacat agctacagcc tgctgtcgtg
    23341 ggaactcaaa ctggcggcgg tatggggtgg tcatgaaggt tcgctgctgc tttgggttct
    23401 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
    23461 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtagt
    23521 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
    23581 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
    23641 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgg
    23701 tgcctgcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat tcacggcggg
    23761 gatcatcctc ggttcctggt gggcctattg cgaactgggc tggggcggct ggtggttctg
    23821 ggatccggtg gaaaacgcct ctttactacc ctggctttct gccactgcgt tgctgcacag
    23881 tttatccctg acacgccagc gggggatttt tcgccactgg tcgctgttac tggcgatagt
    23941 tactctgatg ctgtcattgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
    24001 tcatgcgttc gcgctggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
    24061 tagccttgcg tctctggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
    24121 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
    24181 gctactgatc gtattggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
    24241 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
    24301 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct
    24361 ggtagctcat gcgggcgtgc tgttatttgc cgctgggatc gtggtttcca gcgtcagccg
    24421 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
    24481 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
    24541 ggcactgttt gaccatcagc aacgcattgg tgaactgatg ccggagcggc gtttttacga
    24601 agcacgtcgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
    24661 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgcttgt atgtacaaag
    24721 cggtgtgcgc tggatctggg ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    24781 atggcggggg aggaagcgcg atgaataaag gacttctcac gctactgctg ttatttacct
    24841 gttttgcgcg cgctcaggtt gtagacacct ggcaattcgc taatccgcaa caacaggaac
    24901 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactgg
    24961 aatccaatgc gccggtggca gtcagtatgc gccatcaggt ttacagcatg gtggcggagg
    25021 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    25081 ataacccacc gttaacgggt cagacgctgg tgttatgggc gctgccagtg gtgttgttac
    25141 tactgatggc actgatcctc tggcgagtga gggcgaagcg atgaaacagc ccaaaatacc
    25201 ggtgaaaatg ctgacaacac tcacaatatt gatggtattt ctctgtgtca gcagttatct
    25261 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    25321 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattacagg ataaaatccg
    25381 cgctaatcca caaaacagcg aacagtgggc gttactgggc gagtactatc tgtggcaaaa
    25441 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacatg gtgagaacgc
    25501 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    25561 ccagactcgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    25621 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    25681 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    25741 taatatggcg aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    25801 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    25861 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    25921 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    25981 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    26041 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    26101 attgaggaag ttattctgat gaaaagtata aaattcagcc tggcctggca gattctgttt
    26161 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    26221 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    26281 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    26341 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    26401 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    26461 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    26521 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    26581 atggcgaaag gcgaaatgct gccgatcatc ttcttctcgg tgctgtttgg tctggggctt
    26641 tcctccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa
    26701 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    26761 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    26821 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    26881 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    26941 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    27001 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    27061 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    27121 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    27181 gctggcgtgc cgggagtgtc gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt
    27241 ccgctggaag gtctggcgtt tattgccggt gttgaccgta tcctcgacat ggcgcgtact
    27301 gcgctgaacg ttgtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    27361 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggtaaatt tgataaaact
    27421 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtgg atgcctgatg
    27481 cgac
//
LOCUS       DACAPB010000046        27194 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.046, whole genome shotgun sequence.
ACCESSION   DACAPB010000046 DACAPB010000000
VERSION     DACAPB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.046"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..85
                     /locus_tag="IT904_001748"
     CDS             <1..85
                     /locus_tag="IT904_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191542.1"
                     /translation="TVRGKSADIHYQVSVDCKAGMAEYQRR"
     gene            412..1071
                     /locus_tag="IT904_001749"
     CDS             412..1071
                     /locus_tag="IT904_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAP3191543.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            1280..3340
                     /gene="ptrB"
                     /locus_tag="IT904_001750"
     CDS             1280..3340
                     /gene="ptrB"
                     /locus_tag="IT904_001750"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAP3191544.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINARPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQEPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(3337..3999)
                     /gene="exoX"
                     /locus_tag="IT904_001751"
     CDS             complement(3337..3999)
                     /gene="exoX"
                     /locus_tag="IT904_001751"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAP3191545.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4023..4679)
                     /locus_tag="IT904_001752"
     CDS             complement(4023..4679)
                     /locus_tag="IT904_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAP3191546.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(4781..5011)
                     /locus_tag="IT904_001753"
     CDS             complement(4781..5011)
                     /locus_tag="IT904_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP3191547.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            5150..5524
                     /gene="yobA"
                     /locus_tag="IT904_001754"
     CDS             5150..5524
                     /gene="yobA"
                     /locus_tag="IT904_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAP3191548.1"
                     /translation="MVSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            5528..6400
                     /gene="copD"
                     /locus_tag="IT904_001755"
     CDS             5528..6400
                     /gene="copD"
                     /locus_tag="IT904_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAP3191549.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            6413..6754
                     /locus_tag="IT904_001756"
     CDS             6413..6754
                     /locus_tag="IT904_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAP3191550.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            7150..7806
                     /gene="pphA"
                     /locus_tag="IT904_001757"
     CDS             7150..7806
                     /gene="pphA"
                     /locus_tag="IT904_001757"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP3191551.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(7807..8082)
                     /locus_tag="IT904_001758"
     CDS             complement(7807..8082)
                     /locus_tag="IT904_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAP3191552.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLHSMSDQRIRQGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(8103..8339)
                     /locus_tag="IT904_001759"
     CDS             complement(8103..8339)
                     /locus_tag="IT904_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAP3191553.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSILYRTRYDQQSDAWIMRLA"
     gene            complement(8457..9896)
                     /gene="rsmF"
                     /locus_tag="IT904_001760"
     CDS             complement(8457..9896)
                     /gene="rsmF"
                     /locus_tag="IT904_001760"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAP3191554.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPAANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(9976..12609)
                     /gene="yebT"
                     /locus_tag="IT904_001761"
     CDS             complement(9976..12609)
                     /gene="yebT"
                     /locus_tag="IT904_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAP3191555.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(12578..13861)
                     /gene="yebS"
                     /locus_tag="IT904_001762"
     CDS             complement(12578..13861)
                     /gene="yebS"
                     /locus_tag="IT904_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAP3191556.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRVRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            13991..14488
                     /locus_tag="IT904_001763"
     CDS             13991..14488
                     /locus_tag="IT904_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP3191557.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            14585..15283
                     /gene="proQ"
                     /locus_tag="IT904_001764"
     CDS             14585..15283
                     /gene="proQ"
                     /locus_tag="IT904_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAP3191558.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            15303..17351
                     /gene="prc"
                     /locus_tag="IT904_001765"
     CDS             15303..17351
                     /gene="prc"
                     /locus_tag="IT904_001765"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAP3191559.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ALLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            17543..18424
                     /gene="htpX"
                     /locus_tag="IT904_001766"
     CDS             17543..18424
                     /gene="htpX"
                     /locus_tag="IT904_001766"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAP3191560.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(18470..19843)
                     /locus_tag="IT904_001767"
     CDS             complement(18470..19843)
                     /locus_tag="IT904_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191561.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            20020..20811
                     /gene="kdgR"
                     /locus_tag="IT904_001768"
     CDS             20020..20811
                     /gene="kdgR"
                     /locus_tag="IT904_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAP3191562.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(20954..21193)
                     /locus_tag="IT904_001769"
     CDS             complement(20954..21193)
                     /locus_tag="IT904_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191563.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            21352..21495
                     /gene="mgrB"
                     /locus_tag="IT904_001770"
     CDS             21352..21495
                     /gene="mgrB"
                     /locus_tag="IT904_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAP3191564.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            21570..21857
                     /locus_tag="IT904_001771"
     CDS             21570..21857
                     /locus_tag="IT904_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191565.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            22526..22669
                     /locus_tag="IT904_001772"
     CDS             22526..22669
                     /locus_tag="IT904_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAP3191566.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            22682..22891
                     /gene="cspE"
                     /locus_tag="IT904_001773"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             22682..22891
                     /gene="cspE"
                     /locus_tag="IT904_001773"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP3191567.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            23057..23866
                     /gene="rlmA"
                     /locus_tag="IT904_001774"
     CDS             23057..23866
                     /gene="rlmA"
                     /locus_tag="IT904_001774"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAP3191568.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(23863..24429)
                     /gene="mntP"
                     /locus_tag="IT904_001775"
     CDS             complement(23863..24429)
                     /gene="mntP"
                     /locus_tag="IT904_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAP3191569.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(24858..25316)
                     /locus_tag="IT904_001776"
     CDS             complement(24858..25316)
                     /locus_tag="IT904_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAP3191570.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(25371..26222)
                     /locus_tag="IT904_001777"
     CDS             complement(25371..26222)
                     /locus_tag="IT904_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAP3191571.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(26235..27035)
                     /locus_tag="IT904_001778"
     CDS             complement(26235..27035)
                     /locus_tag="IT904_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP3191572.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(27098..>27194)
                     /locus_tag="IT904_001779"
     CDS             complement(27098..>27194)
                     /locus_tag="IT904_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; subunit IIA transfers a phosphoryl group to
                     subunit IIB; subunit IIB transfers the phosphoryl group to
                     the substrate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP3191573.1"
                     /translation="KLNAHGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT         7112 a         6610 c         6864 g         6608 t
ORIGIN      
        1 aaccgtgcgt ggtaaaagtg ccgatattca ttaccaggtc agcgtggact gcaaagcggg
       61 aatggcggaa tatcagcggc gttaatggct tctgataatt cgattgaccg ttggcggatg
      121 cggcgcgagt gccttgtccg ccccagaacg caccacaatc gtaggcctga gaagacgcaa
      181 cagcgtcgca tcgggcaatc agcatcaaac aatgccactc aaaacgacaa actactttca
      241 cctaaaatac ccttatcacc tgatatacag taaatacctt acctggcgcg aatttctttg
      301 aataacggcc taccgccccg cttatttgcg catcttttgt ctcgatatac attttctgac
      361 atcctcatcc gctatgctac aggcactaaa aatggttatt gaggagcaaa tatggctaac
      421 tggttaaatc aactgcaatc cctgcttggg caaagcagtt cttctacctc ctcgtctgcg
      481 gatcagggat tgggcaaact gttagtgcca ggcgcattag gcggactggc tgggctgctg
      541 gtcgcaaata aatcagcacg taaacttctt acgaaatatg gcaccaacgc gttactggtt
      601 ggcggcggag cggtagcggg tacggtgctg tggaataaat acaaagataa aattcgcgcg
      661 gcgcatcagg acgaaccgca gtttggcgcg caaagtacgc cgctggatga gcgtacagaa
      721 cgtttgatcc ttgcgctggt ctttgccgct aaaagtgatg gtcatattga tgccaaagaa
      781 cgtgcggcaa tcgaccagca attgcgtgaa gccggcgtgg aagagcaggg gcgtgtactc
      841 attgagcagg caatcgaaca accgctggat ccacaacgcc tggctaccgg ggtccgcaat
      901 gaagaagagg cgctggaaat ctatttcctg agttgcgcgg ctattgatat tgaccatttt
      961 atggaacgca gttacctgaa tgcactgggt gacgcgctga aaatccccca ggatgttcgt
     1021 gaaggcattg aacgcgatct cgaacagcaa aaacgcactt tggcggaata atcccgctct
     1081 ctggatagcc tgaagttgac tatttcgggc tatccagaac cgcacagcca tttgaacagg
     1141 ctgattttct ctgtctgcat aacattcgat attcgcgcca ttacctgtca tcatctaagc
     1201 aatgactgcc ctgtttcgct tgcatccccg gtgagttttg ccacccttat aagatgtttc
     1261 aaccagaaag aacaataaca tgctaccaaa agccgcccgc attccccacg ccatgacgct
     1321 tcatggcgat acgcgcatcg ataattacta ctggctgcgg gacgatacgc gttctcagcc
     1381 ggaagtcctg gactacctgc aacaagaaaa tagttacggt catcgggtga tggcctcaca
     1441 acaagccttg caggatcgca tcttaaagga aatcatcgac cgcattccgc aaagagaagt
     1501 ttctgcgccc tacatcaaaa atggctaccg ctatcggcat atttatgaac caggctgtga
     1561 atatgctatc taccagcgtc aatcggcgtt cagtgaagag tgggacgagt gggaaatatt
     1621 gctcgatgcc aacaagcgcg cggctcatag tgagttttat tcgatgggcg gaatggcgat
     1681 tacgcccgat aacaccatta tggcgctggc agaagatttt ctttcccgac gccagtacgg
     1741 cattcgtttt cgtaatctgg aaacaggtaa ctggtacccg gaactgctgg ataacgttga
     1801 acccagcttt gtctgggcaa atgactcctg gactttctac tatgttcgca agcatccagt
     1861 gacgctgctg ccttatcagg tctggcgtca cgctatcggt actccagcat cgcaagataa
     1921 actgatctac gaagaaaaag acgataccta ttacgtcagc ctgcataaaa cgacgtcgaa
     1981 gcactatgta gtcattcatt tggccagcgc caccaccagt gaagttcgcc tgctggacgc
     2041 ggaaatggcc gatgccgagc cgtttgtttt tctgccgcgc cgcaaagatc acgaatacag
     2101 ccttgatcac taccagcatc ggttttatct gcgttccaac cgcaacggca aaaactttgg
     2161 cttataccgt acccgtatgc gtgatgagca acagtgggaa gagttaattc cgccacgcga
     2221 aaacatcatg ctggaagggt ttacgctgtt taccgactgg ctggtggttg aagagcgtca
     2281 gcgcgggtta accagtttgc gccaaattaa ccgcaagacc cgggaagtca ttggtattgc
     2341 ctttgatgat ccggcctatg tgacctggat tgcctacaat ccagaatctg aaaccgcgcg
     2401 attgcgttat ggttattctt ccatgaccac accagacact ttgtttgaac tggatatgga
     2461 taccggtgag cgtcgtgtat taaaacaaac ggaagttcct ggttttgatg cggcgaatta
     2521 ccgcagtgaa cacctgtgga tagtcgcccg tgatggcgtc gaagttccgg tttcgctggt
     2581 ctatcatcgc aaacattttc gcaaaggaca caacccgctg ctggtgtatg gctatggttc
     2641 ttacggcgca agtattgatg ccgatttcag ttttagccgc ttgagtttgt tagatcgtgg
     2701 ctttgtctac gccattgtcc atgttcgcgg cggtggtgag ctggggcaac aatggtacga
     2761 agacggaaaa tttctgaaga agaaaaatac gtttaatgat tatcttgatg cctgcgatgc
     2821 attgttaaaa ctgggctatg gctctccttc gctttgttat gcgatgggcg ggagtgcggg
     2881 gggcatgttg atgggcgttg cgattaatgc acgccctgaa ttattccacg gcgttatcgc
     2941 ccaggtaccg tttgttgatg ttgtaacaac aatgcttgat gaatcaattc ctcttaccac
     3001 tggtgagttt gaagagtggg ggaatccgca ggaaccgcaa tattacgagt atatgaaaag
     3061 ctacagccca tatgacaacg tcaccgcaca ggcttatccg catttactgg taacgaccgg
     3121 tttgcacgat tctcaggtgc aatattggga accggcaaaa tgggtcgcta aattgcgcga
     3181 gctgaaaacc gatgaccatc ttttattgct ctgtaccgac atggactcag gccatggcgg
     3241 taaatctggt cgctttaaat cgtacgaagg cgtagcgatg gaatatgctt ttctggtcgc
     3301 gctggcgcag ggaacattac ccgctacgcc tgcggattaa gtattttcca gataatgttt
     3361 cagtgttaaa cgcagctccg ggctcatgct gtccaggtta ttaaataacc agcgcagata
     3421 gcccggatcg cgttcggcaa cgtcggaaac cgctttgcca cggtatttgc caaaggtgaa
     3481 ggtcgtcatc aacgacggac gtccggtgat atcggccatc tgttctgccg tccagccgga
     3541 ggtgttcata atatcgataa gcaacgcggc ggtgatataa cagtcataca acgcgcggtg
     3601 atgatgcagg cccggcggcg tctgtacatt gagcttgcgt gttttatata acgccatatt
     3661 gctgtacttg atccctggcc acaaacgacg ggccagtttc atagtgcaaa tccactcacc
     3721 gggcatctca ggcagtacgc ggcggtcaaa gctggcgtta tgcgcgacat accattcact
     3781 accgtaatag tgtgggatca catcttcaat ccacggttta tcggcgacca tggcttcggt
     3841 gatgcgatga atcgccatcg cttgtggact aataggacga tcggggcgca ccaggtggct
     3901 catggggttg acgatttttc cgtcaatgac atcaacagag gcaatctcaa cgatccctcc
     3961 ctgcaaaccg caggtttctg tatcgataat gcgcaacatg gcatgctcca ggccgaaaaa
     4021 gcctagcgta atggaatgat atcgccttgc caaccctgtg atgaacgctg accgactaac
     4081 aataatgaac cacgatcggc gcgaacgatg agacgaccat gttcatccca tagtgcactg
     4141 ttaccacggg catttgccat cagcacggca atggagtact gatgtgaaaa aaattgtaag
     4201 cggcgggtag atgccagtag gtcgggttcc cccagacatt gccctgtcgt aaacagtgaa
     4261 caggtcgggt ccatatccat accttgcggt tgttcatcta ccaccgtgat ggttctggaa
     4321 cgacggccca gacatgcacc atgactctga tggtagatcc ccggcgtttt tcgccacggt
     4381 gcgaacaccg caattccacg aataaagcga tcgttatatt caacgggaag gccggctatg
     4441 atggtcattc gccaggtcgt tgcggcataa caaagcgggt ctaacagtga gagatcgggt
     4501 ggggcaggaa gggcacgtcg cgaataatca caccccagta aagaaagcga tggaaaaact
     4561 aacagctgac attgttgcct ggcggcggcc ctcacaaact ccagatgatg agctacctgt
     4621 tcggtgagtg aggttttacg aggctcatat tgcgctgctg caactttcca gaacgacata
     4681 atgacttcct tttcaaaatg actacaaatt atgggtgcgg tttagaaaat actcataatt
     4741 tagcaggcgt tatgtaagaa agtgtaactc tgtataagtt ttatttaagt ttgggctcgt
     4801 aaggtaaacg tgacagattg accgaagcca aacggtgggc aataagccgc tcgcgaaacc
     4861 agctgcgcaa atgttcaggc tgttcacgtt caaccgcttc agcgatcacc ggcatattgt
     4921 agcgttcttt aaatgccacc ccggccgccg ccaaatcgac attcacttta tccatttctg
     4981 tttgatccag tttagccaga ttcttcagca ttcttaatct cctcatcatt cgcgggcaaa
     5041 agttactaag agtgagcctg gatggcaagc cggaattgat gatcctcgac tcagacatta
     5101 gacaaggtta tgctctgtaa aaaagttatt acaaaaagga atagataata tggtttcaac
     5161 tgcacgctcc cttcgctacg cgctggcaat cctgaccact tcgttggtga caccttccgt
     5221 ctgggcacat gcgcatttaa cgcatcagta tcctgcggca aacgcgcaag tgacagctgc
     5281 cccgcaggca atcactttaa acttctcgga aggtgttgaa acaggattca gcggtgcaaa
     5341 aatcacgggg ccaaaaaacg aaaatattaa aacattaccg gcgaagcgaa atgagcagga
     5401 ccaaaagcaa ctgattgttc cactggccga ttcgctgaaa cccggaacct acaccgttga
     5461 ctggcatgtt gtttcggtgg acgggcataa aacgaaagga cactacacct ttagcgtgaa
     5521 ataaatcatg ctggcgttta cctggatcgc gctgcgattt atccatttca cctcgctgat
     5581 gctggttttt ggcttcgcaa tgtacggtgc ctggctggcc cccttaacga ttcgtcgctt
     5641 gctggcgaag cgttttctgc gtttacaaca gcacgctgcc gtctggagtt tgatcagtgc
     5701 cactgcaatg ctcgccgttc agggcgggtt gatgggaacg ggatggacag atgtattttc
     5761 gccaaacatt tggcaggctg ttttgcaaac gcagtttggt ggcgtctggt tatggcaaat
     5821 tgttctcgcc ctcgtaacgt tgatcgtcgc ccttatgcaa ccgcggaata tgccacgttt
     5881 gctgtttatg ctcactaccg cgcaatttat tctgctggcg ggagtggggc atgcgacgct
     5941 gaatgaaggg gtaacggcga aaatccatca gactaatcac gcgattcacc tgatttgtgc
     6001 ggcggcgtgg tttgggggat tactgccggt gctctggtgt atgcaactca tcaaaggccg
     6061 ttggcgacac caggctattc aggcgctgat gcgtttttcc tggtgcgggc attttgcggt
     6121 gatcggcgta ctggcaagcg gcgtgcttaa tgcattgttg attaccggat ttccccccac
     6181 acttacgact tactggggcc aactcttatt gctcaaagct atcctggtga tgattatggt
     6241 ggtcatcgcg ctggctaatc gttatgttct tgtaccgcgc atgaggcagg atgaagatcg
     6301 tgcggcaccc tggtttgtgt ggatgactaa actcgaatgg gcgataggtg cggtagtgct
     6361 ggtgatcatc agcctgcttg cgactctcga acctttttga tggattacga aaatgatgaa
     6421 aaaaagtatt ctggcgtttc tgttactcac cagttctgca gcggcgctgg cggcaccgca
     6481 ggtgattacc gtcagccgtt ttgaagtggg taaagacaaa tgggcgttta atcgcgaaga
     6541 ggtgatgctg acttgccgac cgggtaatgc tttgtatgtc atcaacccaa gtaccctcgt
     6601 gcagtatcct ttaaacgata tcgcacaaaa ggaagttgcc agtgggaaga ctaaagccca
     6661 acccatttcg gtgattcaga ttgatgatcc taacaatccc ggcgaaaaaa tgagtctggc
     6721 accgtttata gaacgagctg aaaaactctg ttaattacct aaaatagcct tttgatttcc
     6781 aataaaaaaa ccgcctcagt tctttcacca gaacgggcgg tttttaacat ttaagctgat
     6841 gaccaccgcg ctttttattg accattttgc acgcaaactg gaaaacctgg cgtcgtcatc
     6901 tattcttaaa gggcaaggca actaagcctg cattaatgcc aacttttagc gcacggctct
     6961 ctcccaagag ccatttccct ggaccgaata caggaatcgt gttcggtctc tttttatctg
     7021 ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac acactctact
     7081 gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta gcgcgggagg
     7141 aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga
     7201 catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg
     7261 cattgtcgtt ttgatccgtg gcgagattta cttatctcag tgggagacgt tatcgatcgt
     7321 gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggttcg tgcggtaaga
     7381 ggcaatcatg aacagatggc gatggatgcg ctggcatccc agcagatgtc tttgtggttg
     7441 atgaatggcg gcgactggtt tattgcgctg gcagataatc aacagaaaca agcgaaaacg
     7501 gcgctggaaa aatgtcagca tttgcccttt attcttgaag tacacagccg caccggcaaa
     7561 catgttattg ctcatgccga ttatccagat gatgtttatg aatggcaaaa ggacgttgat
     7621 ttgcatcagg tcttgtggag ccgctcacga ttaggtgaac gcccaaaagg gcagggaatt
     7681 acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt ggatattggc
     7741 aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct tgtgcaattg
     7801 caataattaa aaatcaccgt actcctgcgc aggtcgccag aaaccatcta taaaatcctc
     7861 aatcggaaaa caaccgccct ggcggatccg ttgatcgctc atagaatgaa gacactgctg
     7921 ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt agcaaacaaa
     7981 aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa gtttaggaga
     8041 gattttacaa cgggggaata accaggacaa ataacccgcc atgccgacgg gttctttttg
     8101 gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt acggtaaagg
     8161 atagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca ggcatccagt
     8221 tgcatacata aatcggggtc agatttacaa ggaatggtta acgttcgatc acccggcgat
     8281 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
     8341 ttcgtgctct ccatttagct gccgattgtg gtacgaccag tccgatcgca ccatttttca
     8401 gcctagttga ggccgccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcat
     8461 gcgttactgg taaaaagttt cccgtcccgc accagttcac gcgggtagct gttttttagt
     8521 cgcgaaccaa tccgtttggc taaaccaatc ggctgatgct ggaaagtaac caacacgtca
     8581 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
     8641 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
     8701 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
     8761 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
     8821 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
     8881 tgtccagctt cgcgatcttt caccgggctg aacggaaaat taccgacttt gtatttgggg
     8941 gcgggtaagg cgggaatggc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
     9001 tcgtaaattt gtgggaaaac atgcaaaaag ccttcttcgg tcagcgcttt gtttgcagca
     9061 gggaagagct cgccaagtgg taaaaactct actgcgtcag ggtaagtctc tttcagccac
     9121 atgcaaacgg cttcgttttc ttccctgttt aaggtacagg tcgagtaaac cagcgtacca
     9181 ccaggacgta atgcatgaaa ggcgctgtcg ataagctccc gttgtgtagc tgcgatttcc
     9241 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     9301 ccagagcagg gagcgtccag caaaatggca tcgaacattt ctggcactgc cacaccaaac
     9361 acgcggccat caaaatgtgt gagcgcaaca ttactgatgc cacagcggct gatattggca
     9421 tgtaacactt ttacccgact ggcggaaaac tcattggcaa ggattgcccc ttcgttattc
     9481 atccgtgcgg caatttgcgt cgttttggag cctggcgcag cagcgacatc catcacccgc
     9541 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     9601 atataaaaca ggccgcttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     9661 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     9721 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     9781 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     9841 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     9901 atataccact ggcaaaaaaa caggcgcgca gtttagcata aacgctccgg cgcaggcacg
     9961 ccggagcagt gggcattatt tgggaagcgc agtaccccat tcacgccact ctttcggttc
    10021 actttcctgc aacaggaagt gcttgccttc ctgggctttc ggtgccagtg gcgtccccgg
    10081 aggcgtggcg aaggcgatgc cgccacggat aaactggtta aaggtgccgg ttttcactac
    10141 gccgcccgtc agaccaaagt ccagactgta acccgatgcc aaccagaaga cggaattgtt
    10201 acgcaccagg tgttgatagc gtttactgat gcgcatcgca atcatcacgc gatctgacaa
    10261 tgtccccagc gtcattcctg taaccgtacc gacttccaga ccacggaaca gcacaggcgt
    10321 accgatgcct aacgaaccgg cttccggcgc ttcaacaata atgcttaagc catccaggta
    10381 acgcgaatca gtaatggtgg cctcttgtaa ttcaaagtcg cggcgaggat tgccccggcc
    10441 tggttcgacg ttgatatacg gctggaggat agtatcaaga tgctcaacgc cagctgccga
    10501 aatttgcggt gtgaccactg agaagcgcgt accaccgcga gcaaaggtct ggacatattc
    10561 cggatagagc accgcctttg cctgtacttc attgcgcgcg gtaatcagat ccagcgtctg
    10621 gatttgcccg atatcaatac cgagatagcg aattggcatc ccgaccgcca gttttccggc
    10681 atcgaaagcg tgaagcgtaa tctgcccacc aaccgcacgg gccgctgttt cggaagcata
    10741 cagaattcgt ttgtcgcctt tacgctgact ggcgctggca ccgctgaggt tatcgaagct
    10801 aatggctccc tttaatgctc tggagagcgg ggatgcctgt acggtcaggc cactaccatt
    10861 cagctgaact ttcgccccgc cttctgccca gaacacgcta ttgctggtca gaaggttgcg
    10921 atactccggc ttaatatgca gatcgatatc aaacgcgtta gctcgcggac ggacggtaat
    10981 aacttcacca acttcaaatt tacggtagag cactaccgat cctgcctgca catccggcag
    11041 cgtctctgca ctcaaactca cggttgtggt gggtaaatcg ctaaggctgt tctccagcgc
    11101 tttttccaga ttggcataca gtggatagct ggctttcatc tcgcctttat cgcccggcag
    11161 aatacgtatc ccgccgttaa tccattctga ggcgctggca ccgagaaact caacgccatc
    11221 cagccccacc ttcacgtcga cacggctgtt gacgacaaat ttgctatcgc cttttaccag
    11281 ttctcgatgc tgaggctcga tggcgacggt aaaggtgacg cctttgctgg tgagtttacg
    11341 atcgataacc tggcctacct gcacgccgtg aagaatgagc ggctgacccg catcaatacc
    11401 gtaactttcc ggtgcggtca gggtcagcgt cagaacatca ggttcatgca gcagtgcttt
    11461 ttcgcctggc acaacaacga actctttgcg tggctcgcca tcgccgggta ccaactcgaa
    11521 ggttttgcca gtcagcaggg cgctgagatt ggcatcgcta agggataatt tcgggttgcg
    11581 taattcgatg cgggtatttt cccgtaacag cgtaacgacg ctgggatcaa cggtcatttc
    11641 tccggtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga cttccagccc
    11701 ctgatacatt aacggcgtcg agtcggcggt taatcctgcc ccactcggca gttccagttt
    11761 tattattacg ccacgctggc tgtgggccag atcttcatac agaccaaagg tatcttccgc
    11821 ctcggcaggt ttcgactctt ctggtgaatc gaaggcaatc gcaccgttaa ccagtgccgc
    11881 cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa cgccggaaac
    11941 gttccagaaa cggctacctt ttttcaccag gtcggtaaaa cgccgctcga tcaggacatc
    12001 aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc ccaccgggat
    12061 cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agatcggggg cttgcaggtg
    12121 gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagtg cgacaaagtg
    12181 atcctgctct ttacctttac ccggcatcat gccgatatag ttaccaccga cgagggcgtc
    12241 cagcccggag acacctgcca acgatgcttt tggcgtcacc agccagaact gagtctcttc
    12301 gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc
    12361 gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt
    12421 acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat taccccggtc
    12481 ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag gcagcagcca
    12541 gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc
    12601 ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca
    12661 aaattaccgc tgcgccgaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat
    12721 cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgac
    12781 caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa
    12841 aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac
    12901 tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac ataattccgg
    12961 aaagaattgt atcttcctgc cgtcctccgt tcagataaat gatagaaata ggcaacaggt
    13021 tggcaggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcaaactat
    13081 gacggcgacg cacgcgtagc gggatatggc aacgcgggca gcgaccacgc tgatcaggat
    13141 agccggtaaa atggcaccca agacagacac ggagtttctc gtccctacgc gtagcggggc
    13201 gctgcggata aaatcgctcc cacagctcct cgacattaag atgtgacaac gtcaccgtcg
    13261 ttaaaatcac caacgccaca aaagagaaca agcccacacc cgcctggata tgggcataat
    13321 cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc attacccact
    13381 ctttaagtcg ctcaagcatc agcagtaccg ggcgtagatt cattcccagt cggttaccaa
    13441 accataaata agctatggag gtcaccagaa tgaggggggc accgataacg cagaaaaaga
    13501 ccatcgaccc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct tgcataacgt
    13561 tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc caggcaaacg
    13621 gcatcagcaa cagcatggtg aaagccattg ccgccaggcg cgttagcgac cagtcgcgcc
    13681 cgtcgcgaat ttttgcctga cagcgcggac aataggcact ctgatgagaa tttatctcgg
    13741 gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct
    13801 cgccgattga cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca
    13861 tgacgtgcca aatgtaataa gaattaacat taatattaac ccatgattgg aatgatcttg
    13921 agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaccaag
    13981 tgattatata atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct
    14041 gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatacga
    14101 gcgtctcact gacgtaaact gggcaggttt ttatttgctt gaggacgata cactggtact
    14161 cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gggtgtgcgg
    14221 cactgcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt ttgacgggca
    14281 tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca
    14341 gattattggt gttcttgaca tcgatagtac cgtcttcggt cgctttacag acgaggacga
    14401 gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa
    14461 aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta ctggtagcgt
    14521 cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa
    14581 tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctggccga
    14641 acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa tcggtatttt
    14701 tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc
    14761 cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac
    14821 gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc
    14881 tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaagc
    14941 gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc gccgcgaacg
    15001 caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca
    15061 aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac agcacacccc
    15121 ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag cgggtcaaaa
    15181 cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc
    15241 gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag
    15301 gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga
    15361 ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc
    15421 agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt
    15481 tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt
    15541 acagccacaa cgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt
    15601 taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc
    15661 aaaagcgccg ttttgagcgt taccagtacg ctttgtcggt actggaaaag ccgatggatt
    15721 tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg
    15781 ctgagttgaa cgcgctgtgg gacagtaaag tcaaattcga cgagttaagc ctgaagctgg
    15841 caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc
    15901 gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg gcgtttgcgc
    15961 gtgaaatcga cccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg
    16021 aaatgagttt gtcgctggaa ggtattggcg cagtgctgca aatggatgat gactacaccg
    16081 ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg
    16141 acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt ggctggcgtc
    16201 ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt
    16261 tacctgctgg taaagggacc aagacccgca ctgtaacatt gacccgtgaa cgtattcgtc
    16321 tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa gtcggcgtgc
    16381 tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa ctgcagaaac
    16441 tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt
    16501 tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt gttcaggtcc
    16561 gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg ttctataaag
    16621 gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc tttgccgcgg
    16681 caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg
    16741 ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag
    16801 cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc ggcagtacgc
    16861 aacgtaaagg cgtaacgcca gacatcatca tgccgacggg taatgaagaa acggaaacgg
    16921 gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg acttatgtga
    16981 aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat gcgcgtatcg
    17041 cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac gctatgaagg
    17101 acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat aatgaagatg
    17161 atgcgacgcg tctggcgctt ttgaacgaac gctttaaacg cgaaggtaaa ccggagttga
    17221 agaagctgga tgatctaccg aaagattacc aggagccgga tccttatctg gatgagacgg
    17281 tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa caacccgctc
    17341 ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac agcggcaaga
    17401 tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact tacgcactat
    17461 ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt gcgttttgtt
    17521 aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa cgaacctggc
    17581 cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga gcagcgttca
    17641 ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt cgcttctgat
    17701 gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac cgcgtaacga
    17761 aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg ggatcgctat
    17821 gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg gtgcgcgccg
    17881 tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc cggatgaagc
    17941 cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg ttaccatgac
    18001 gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc tggcgcagct
    18061 tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg gcaacccgct
    18121 gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg cgagcattat
    18181 caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg caaaactggt
    18241 tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg aaccgcaaga
    18301 agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca gtgagttgtt
    18361 catgactcac ccgccgctgg ataaacgaat tgaagctctg cgtacgggtg aatacctgaa
    18421 gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt tatgccctgg
    18481 atcgtggctg agtgatacgt aaaccactca cacaggcagc aatcactgcc agaatagccg
    18541 cagccatcag cgaaacgtgc gtaccattat caccgaactg atttagcatc agcgccacca
    18601 gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca ctggccccac
    18661 cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac tggaataagc
    18721 caaatccagc gccacataag atcatcggcc agataatatt gatatccgca ggtgacgcgg
    18781 gcagcagaac cagggaaaaa agccccgcag ccatgatgaa caaccctaaa gcacccagca
    18841 atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc atcgttgcta
    18901 acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc acggtttgca
    18961 ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa acagatgtgc
    19021 aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc ggtacgggaa
    19081 gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc agttccgcag
    19141 caattaacgt tagcgattgc ccctgagcga aaccactcaa cgcagtgata agcaggccga
    19201 aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg gcgcgagaac
    19261 cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac ggtacgttga
    19321 ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt gtcggcccgg
    19381 cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc agaaaacgtt
    19441 gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca ccgccgaaac
    19501 cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg cagaacagtg
    19561 aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca aacatatcgc
    19621 ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc
    19681 agatggaact ggctggcgtg gcatgaaggt ccgtggcgat tgttggcagg gcgacgttgg
    19741 cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat atcgcaccgt
    19801 atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt ttatatcttt
    19861 ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat
    19921 tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat aataaaaaac
    19981 agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat
    20041 aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt
    20101 gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc
    20161 gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg
    20221 gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc
    20281 gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact
    20341 atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg
    20401 tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt
    20461 ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct tgagggcgtg
    20521 gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg
    20581 gaccaggtgc gcgagcaggg gtatggcgaa gataacgaag agcaggaaga agggctgcga
    20641 tgcattgcgg taccggtatt tgatcgcttt ggtgtggtca ttgccggttt gagcatctcc
    20701 ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat gttgcatacc
    20761 gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg atgagagtaa
    20821 gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa ataaagttga
    20881 aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt gagaatctgg
    20941 ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac agtgggcaat
    21001 ctgttatccc caagaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg
    21061 cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt ccaatcaaaa
    21121 cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa
    21181 tgatgaatcg cattacaacc tcttctcttt ttatgttcgc ttaatcgtag cggcaatatg
    21241 cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta ggcgctgttt
    21301 aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg agtgaaaaag
    21361 tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg ggcgcaggta
    21421 ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc cattaaccag
    21481 tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc agcagtggta
    21541 gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca ggcgtgatga
    21601 acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc ttgtggtttt
    21661 ttatcaatcg tgccagttct cggactaacg aacagattga actgcttgag gcgttgctgg
    21721 atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac gaacctgaga
    21781 aagcagataa aaagaccgtt gagagtcaaa aatcagttga agacgaagat attattcgtc
    21841 tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga agggttactc
    21901 aattcatcct gattatacgc actgccatgg tgcaactgct tcaaattaaa actctggtct
    21961 gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa aagatatttt
    22021 cctcattaat ttattaggat gtttacatcg gatttgtgat taagcgtggt attatttatt
    22081 agcgaaacgt ttctctcttg attttttgct cattcatcaa tttttcttat tttaaatttt
    22141 caatcccttt ggggattgac ttctctttag ggtaattgat agccgttaac tgactgtttt
    22201 atgagaaaaa gtgatataac tttttattca ttgcatagca aaaaatgtga tattgcacgc
    22261 actatgtaat aacttctccc actggcctgg aacaactgaa cttattgaac tatgttagaa
    22321 actacgccag tttaagtatc tgcctgaact ggcaaggtta agcacaatga tatatcggcg
    22381 cgtattccgt tgcataagtg tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg
    22441 atcgagacat gtttaaaaat ggcttgccat aattaacgtt gtatgtgata acagatttcg
    22501 ggttaaacga ggtacagttc tgtttatgtg tggcattttc agtaaagaag tcctgagtaa
    22561 acacgttgac gttgaatacc gcttctctgc cgagccttat attggtgcct catgcagtaa
    22621 tgtgtcagtt ttatctatgt tatgcctgcg ggcgaagaaa acaatctaag gaatttttca
    22681 aatggcaaag attaaaggtc aggttaagtg gttcaacgag tctaaaggtt ttggcttcat
    22741 tactccggct gatggcagca aagacgtgtt cgtacacttc tccgctatcc agggtaatgg
    22801 cttcaaaact ctggctgaag gtcagaacgt tgagttcgaa attcaggacg gccagaaagg
    22861 tccggcagct gttaacgtaa cagctatctg atcgaatcca ctgatctgaa gtgtgaatac
    22921 gcttcaatct cgctataaag cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg
    22981 ctcctggcgt tcggatattt gcccgccgcg tgattcgcgt tacacttgcg gcctttagta
    23041 tcctgccgga gttgtcatgt ctttttcctg tccactttgc catcagcctc tttcgcgtga
    23101 aaaaaacagc tatatctgtc cccagcgaca tcagtttgat atggcgaaag aagggtatgt
    23161 caatctgctg cccgttcagc ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat
    23221 gcaagcacgc cgcgcattct tagatgccgg acattatcag ccgctgcgtg atgcaattgt
    23281 cgcccaactg agggaacggc ttgatgataa ggccacggcg gtgctggata ttggctgtgg
    23341 tgaagggtat tacacacacg catttgccga tgcgttgccc gaaatcacca cgtttggtct
    23401 ggatgtttcg aaggtagcga taaaagcggc ggcgaaacgc tatccgcagg tcactttttg
    23461 tgtcgcttcc agccaccgtt tgccgttttc cgataccagt atggacgcca taatacgtat
    23521 ttacgcgccg tgtaaagcag aagaattagc acgagtagtg aagcccggcg gctgggtcat
    23581 tactgccacg ccgggaccgc gacatttgat ggagctgaag gggctgattt acaatgaagt
    23641 acatcttcat gcacctcatg cagaacaact ggaaggtttt acattacagc agagtgcgga
    23701 gttgtgttat ccgatgcgtc ttcgcggtga tgaagccgtc gcattattgc agatgacgcc
    23761 gtttgcctgg cgtgcgaagc cagaagtctg gcaaacactg gcagcaaaag aagtgttcga
    23821 ctgccagacg gactttaata ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt
    23881 ccagaggatc tggacgccga tgccgatcag taccagcccg ccgagaattt ccgctttttt
    23941 cccaataatt gagccgataa agcgaccaac catcatccct aatgttgaca taatcaaggt
    24001 tgcacaacca atggccaatg cggtcgcgat aatgttgacc tgcaggaaag caagaccaac
    24061 acccacagcc atggcatcca ggctggtggc aatcgcggtg gttaccagta gccagaaacc
    24121 gtgtcgacgg cgcggctctt catcttcatc atctgcgcca cgaaaaccct caataatcat
    24181 tcgcccgccg aggaatatca gcagcacaaa cgcaatccag tggttccatt caaggacaaa
    24241 ccggctggct aacatgccca ttccccagcc gatcagcggc gtcagggttt cgacggcacc
    24301 aaaaataagg ccggttcgca atgcttcaga aaatttcggt ttatggaggg tggcaccttt
    24361 accgattgat gcagcaaatg catccatcga cataccaaac gcaagaagaa cagtagcagt
    24421 gatattcatg acaatgtcct gaccggggta tccataaaac acatcatcgc ccccagcaaa
    24481 catgcataca tgaacaacaa gccgcgatgg ttttcgcgtt catgcaggca tagcgattga
    24541 tgtgcatatg gtctcgcctg actgaaagta ttcagtccgt acgtgccacg ttttgcaacg
    24601 agtatgttga cacgtacatt tccggaatct ggaaatcggc tactccccaa atgagggcgc
    24661 aaccttaaca tattttgaga atataaaaca actgttggaa tatattaatt cattgcttta
    24721 ttgataacga tttacattta gatttatata tcaatgaaac gttaaaataa ataacaaata
    24781 agggaagagg taatatagcc taagctatgt ctgattatta aaaatacact aaacatagct
    24841 ttggctaaat tcataactta ttgagttgaa acgagaagct tataaatttt ttccagatcg
    24901 tcgatatttc gaacgcggat taacagccga cgctgttcta attgcatcac caacacgcca
    24961 tcttccgaca agttcatcgc tttgattcgg ctatattcaa tccagacatt ggcgaagaaa
    25021 aaaccttttt gtttaaagat gatcttcgga acgcggatcc agaatatata aaaacccatc
    25081 agagccagtg cgcttaataa ccatgtcgtt attaacgcac catgattcgt gacgttgtta
    25141 tagataagaa tgacaatcaa tccgacgaag ataacgctat cgatgcgacc acgccggagc
    25201 aaaggaattg ccagcagggt ggggccatta cggcggggca tgatgaactg atcgtagatc
    25261 gcgaaggcca ggagtgcggc gatgaaaaga atcagtacca ggtccgtgat tgtcattaat
    25321 cctccagata aaaaaacggg gccaaaaggc cccggtagtg tacaacagtc ttacagtccc
    25381 agcaggccgc aagcgtaacc agcgataccg atgacgaaga agccaacgat gatccacagc
    25441 gggttaactt ttttgcgcag tagccacata caagcaaagg tcagcagcag tggtaccagg
    25501 cctggcatta actggtccag aatagtctgg acagtagtaa cgtgttcttt gcccgtctgg
    25561 tcagtaatgc gagagacaac cagcgggatg ttgacatgtg tccacttgtt aaccaatgcc
    25621 cccatgacaa acaggccgag gatagacgcc ccttccgtca gtttttgcag gaagccacca
    25681 cccatatctt taacgatatc gatacctttg gagtaaccat acgctacgcc gtagtaacgg
    25741 gttgccagac gcaccaggtt aaacaggatg aagaacagca gcggacctaa caggctgccg
    25801 ctcatcgcga taccggcacc cagtgctgca aataccggac gtacggttcc ccagaagatc
    25861 gggtcgccta caccagccag tggccccatc aaaccgactt tgataccgtt gatagcaccg
    25921 tcgtcgatct ctgcgccatt agcacgctgt tcttccagcg ccagggttac gccgagaatc
    25981 ggcgcagcca cgaacggctg ggtgttaaag aactccaggt gacggcgaat agcttgttta
    26041 cgagcttcgt tgttctcagg gtagaggcga cgaattgccg gtaccataga gaagcagaaa
    26101 cccagtgcct gcatacgttc gaagttccat gaaccctgga agaggttaga acgcaggaag
    26161 acgccacgaa tatcactttg agtgagtttt ttctcggtgg tagtttgagt tgtatcaacc
    26221 atttcgctca cctgttagtc cagttcgtta tcgagatcgt tgttaccagc tgcctgagca
    26281 ggcgcaccgg ctacgcggtt gtatttcggg ctaagttgga tgtagagcac tgccataaca
    26341 gtaccaatca cacccagagc aaccaggtta aagttggtga atgctgcggt tacgaagccg
    26401 aggtagaaga acggcatcag gtagccagca cgcatcatgt tgataaccat cgcataacca
    26461 accaccacga tcatgccacc ggcgatattc agaccattgg tcaccacttc cggaatcgca
    26521 ttcagcatgt tctgtacttc gctggtacca acagacagcg caacgatgac ggccggaata
    26581 gccacacgca ttgcttgcag gaacagagaa gaaacgtgga tccaggaaat cgctgtcagg
    26641 ttgccgttat cagcagcctt atccgcagcg tgctggaaag caacggtaat agtacgaacg
    26701 atgatggtca gtacctggcc cgcagcggcc agagggattg ccagtgcgat acctgcacca
    26761 atgctctgat gacctgcgat aaccagaatg gtagaaatga tagaagccag agcggcgtca
    26821 ggcgcaactg cagcaccgat gttcatccag cccagcgcga tcatttccag cgtaccaccg
    26881 ataataatac cggttttcat atccccaaga acgataccca ccagggtaca cgcgattagc
    26941 ggacggtgaa actgaaattc atcgaggatt gatcccatac ctgcgataca ggctacgata
    27001 aataccagca caatttgaag agtggtaatc tccattgtac ttctcctgtt tacgtaagac
    27061 ttaagtgtga aaactgagtg ataatcaaca caatacgtta cttatcgatt ttgctgatca
    27121 gatccatcat tttcagtttc ggatcggtgg aaaccttacg gacttccagc tcaataccgt
    27181 gcgcattcag tttc
//
LOCUS       DACAPB010000047        26642 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.047, whole genome shotgun sequence.
ACCESSION   DACAPB010000047 DACAPB010000000
VERSION     DACAPB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.047"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(44..892)
                     /locus_tag="IT904_001780"
     CDS             complement(44..892)
                     /locus_tag="IT904_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAP3191574.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVISF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(914..2926)
                     /locus_tag="IT904_001781"
     CDS             complement(914..2926)
                     /locus_tag="IT904_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAP3191575.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PLNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQLDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LKNVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(2956..3369)
                     /gene="hyfJ"
                     /locus_tag="IT904_001782"
     CDS             complement(2956..3369)
                     /gene="hyfJ"
                     /locus_tag="IT904_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAP3191576.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIT"
     gene            complement(3362..4120)
                     /gene="hyfI"
                     /locus_tag="IT904_001783"
     CDS             complement(3362..4120)
                     /gene="hyfI"
                     /locus_tag="IT904_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAP3191577.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4117..4656)
                     /gene="hyfH"
                     /locus_tag="IT904_001784"
     CDS             complement(4117..4656)
                     /gene="hyfH"
                     /locus_tag="IT904_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAP3191578.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAQQKTVALATELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4666..6333)
                     /gene="hyfG"
                     /locus_tag="IT904_001785"
     CDS             complement(4666..6333)
                     /gene="hyfG"
                     /locus_tag="IT904_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HAP3191579.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(6323..7903)
                     /gene="hyfF"
                     /locus_tag="IT904_001786"
     CDS             complement(6323..7903)
                     /gene="hyfF"
                     /locus_tag="IT904_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAP3191580.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGIYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFLLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7908..8558)
                     /gene="hyfE"
                     /locus_tag="IT904_001787"
     CDS             complement(7908..8558)
                     /gene="hyfE"
                     /locus_tag="IT904_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAP3191581.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8570..9922)
                     /locus_tag="IT904_001788"
     CDS             complement(8570..9922)
                     /locus_tag="IT904_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAP3191582.1"
                     /translation="MGVLFAALTTLCMLSLISTFYQADKVAVTLTLVNVGDVALFGLV
                     IDRVSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQRSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFAAGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9898..10008)
                     /locus_tag="IT904_001789"
     CDS             complement(9898..10008)
                     /locus_tag="IT904_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HAP3191583.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(10025..10972)
                     /gene="hyfC"
                     /locus_tag="IT904_001790"
     CDS             complement(10025..10972)
                     /gene="hyfC"
                     /locus_tag="IT904_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAP3191584.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10983..13000)
                     /gene="hyfB"
                     /locus_tag="IT904_001791"
                     /pseudo
     CDS             complement(10983..13000)
                     /gene="hyfB"
                     /locus_tag="IT904_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13000..13617)
                     /gene="hyfA"
                     /locus_tag="IT904_001792"
     CDS             complement(13000..13617)
                     /gene="hyfA"
                     /locus_tag="IT904_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAP3191585.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVYHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(13870..14340)
                     /gene="bcp"
                     /locus_tag="IT904_001793"
     CDS             complement(13870..14340)
                     /gene="bcp"
                     /locus_tag="IT904_001793"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAP3191586.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14340..14912)
                     /locus_tag="IT904_001794"
     CDS             complement(14340..14912)
                     /locus_tag="IT904_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAP3191587.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15058..15936
                     /gene="dapA"
                     /locus_tag="IT904_001795"
     CDS             15058..15936
                     /gene="dapA"
                     /locus_tag="IT904_001795"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP3191588.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQIC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15953..16987
                     /gene="bamC"
                     /locus_tag="IT904_001796"
     CDS             15953..16987
                     /gene="bamC"
                     /locus_tag="IT904_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAP3191589.1"
                     /translation="MTYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSMEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17200..17913
                     /gene="purC"
                     /locus_tag="IT904_001797"
     CDS             17200..17913
                     /gene="purC"
                     /locus_tag="IT904_001797"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP3191590.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18081..18944
                     /locus_tag="IT904_001798"
     CDS             18081..18944
                     /locus_tag="IT904_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAP3191591.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            18959..20974
                     /locus_tag="IT904_001799"
     CDS             18959..20974
                     /locus_tag="IT904_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAP3191592.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNNVRTERLRDRITQWQFFH"
     gene            21048..21746
                     /gene="ypfH"
                     /locus_tag="IT904_001800"
     CDS             21048..21746
                     /gene="ypfH"
                     /locus_tag="IT904_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP3191593.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21856..22056)
                     /locus_tag="IT904_001801"
     CDS             complement(21856..22056)
                     /locus_tag="IT904_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAP3191594.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22084..23211)
                     /gene="dapE"
                     /locus_tag="IT904_001802"
     CDS             complement(22084..23211)
                     /gene="dapE"
                     /locus_tag="IT904_001802"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAP3191595.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23215..23571)
                     /locus_tag="IT904_001803"
     CDS             complement(23215..23571)
                     /locus_tag="IT904_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAP3191596.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23952..24011
                     /gene="ypfM"
                     /locus_tag="IT904_001804"
     CDS             23952..24011
                     /gene="ypfM"
                     /locus_tag="IT904_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAP3191597.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(24110..>26642)
                     /locus_tag="IT904_001805"
     CDS             complement(24110..>26642)
                     /locus_tag="IT904_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAP3191598.1"
                     /translation="SFQMTAKDVTDAIESQNAQIAVGQLGGTPSVDKQALNATINAQS
                     LLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYDYLSRFNGKPASGLGVKLASG
                     ANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVKASIEDVVKTLLEAIALVFLV
                     MYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNTLTMFAMVLAIGLLVDDAIVV
                     VENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGTTGAIYRQF
                     SITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQKGFFAWFNQMFNRNAERYEK
                     GVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLEDRGMFTTSVQLPSGSTQQQT
                     LKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVARMFIRLKDWSERDSKTGTSF
                     AIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDMELQDHAGAGHDALMAARNQLL
                     ALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVAIDDINDTLQTAWGSSYVNDF
                     MDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVPFSAFATSRWETGSPRLERYN
                     GYSAVEIVGEAAPGVSTGTTMDIMESLVKQLPNGFGLEWTAMSYQERLSGAQAPALYA
                     ISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLATWMRRLENDVYFQVGLLTVIG
                     LSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPILMTSLAFIFGVLPMATSTGA
                     GSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRFPLKPRPE"
BASE COUNT         6690 a         7073 c         7067 g         5812 t
ORIGIN      
        1 gaataatctt cagcccgacg aactgtttcg ccgggctgat ttttcaggat tcctgacgta
       61 aatagatagc ccgataacac atactcacca gcactgcacc gccgataata ttacccagca
      121 tcaccgggag cagattggcg gtaataaaat ggcttaccgt cagtgccgga aaattgtctg
      181 cgctactgtg cgccagttgc cagaaggggg gaggggcgaa atggcgaatg gcaatggcga
      241 acgaaatcac aaacaaattg gcgatacagt gctcaaagcc actggcgaca aacagggtga
      301 tgggcaaaat catggcgacg attttgtcgc ataacgaacg cccgcaataa ctcatccaaa
      361 gcgccaggca aaccatcaga ttgcacataa tgccgaggct gacagattca gtaaatgtat
      421 gatgcatttt gccctcggcg cagtgtaaaa ccgcgactcc ccacatcgcg ttttcactca
      481 tcaccagccc ggaaaaccag attaacaaac tgaaacaggc aatacctgcc agattaccgc
      541 aggccaccag aagtgcgtta atcagccaag ttcgccaact aataacgccc cgactttttg
      601 ccatcaccgt cattaccgac gaggtgaaca ggctggtgcc gcaaaccgcc agcaaaataa
      661 agccgagtgt aaagcaaagg ccgcccacca gatgggttaa tgcctgtgaa gacggggcat
      721 cggcgataac ggaaaggtaa aaagtaaatc cgatcgccat aaacacgcca gcggacatcg
      781 ccattaaaaa gaccgacact ttactgcggg caattttatg ggcggcaatc cgttcagcga
      841 ttgccgcttt tctggcgctc aactgcaagt cgaaagagag tttgtttctc atgcatactc
      901 ctgcaaaagc agattacaac acctcgcgaa ccgagatccc cagacgctgc attcgtgaca
      961 gcagcgtggt gcgcttcatc cctaatcgtg tcgccgcgcc acggggcccg gcaacaatgc
     1021 cattggtttc tcgcaatacc tgaacaatgc gctgacgttc ttcttcgtca ttctctggcg
     1081 tcgtcgggtg cagcaactgc gccattgaac tgcgaagcgc tgaatcttca tttagcgtcg
     1141 gcagtaaacg gctttgtcgg acatttagat gtaaattcag actgttacca cgagtcaaca
     1201 gtaccgcccg ctcaatcacg tttttcagct cgcgcacgtt gcccggccaa tcccacgaca
     1261 tcaactggcg tagtgcctcg gtcgggatgg cgtcaattgc gcgattcata tggcgcgcca
     1321 ttttttgcgt gaaatgcttt gctaaaagag ggatatcttc cggacggtcg cgcagcggcg
     1381 gcaattccag tgggaagaca ttcaggcgat aaaagagatc gctgcgaaac tggcgatctt
     1441 caaccatttg ccacaaatca cggttggtgg cggcaatgac tctgacattt accgggatcg
     1501 ttctactccc gccgagacgc tcaatctccc gttcctgcaa tacgcgcagc agtttaggct
     1561 gaagttctaa cggcagatcg ccaatttcat cgagaaacaa cgtgccgcca tcggcaattt
     1621 caaaacgacc acgatgggta ttaatcgcac cagtaaacgc ccctttgtca tgaccgaata
     1681 actcactttc cagaagactg gcggggatgg cagcgcagtt gattttgacc agcggcttgt
     1741 cgcgtcgcgg gctaagttga tggatcgctc tggcgatcac ctctttgccg gttccggttt
     1801 caccgcaaat caacaccgta ctgtcgctct tcgccacaat atctaactgc tggagtaggt
     1861 cttccattgc ctggctttga tagataatgt caccgatgcc cagattcgaa aggagctgct
     1921 cgctaagctg gtggttttct tgctgcaaac tttcctgcaa atcggtcatg ctacgccagg
     1981 catcggcatt gccaacggca atagcgatgc gatcggctat gtgttgtagt agctggcagt
     2041 tttcctcact aaagagagtg gaagaggtat gcgccagcaa caatgcaccc ggtgtatggt
     2101 tgccaaaggt aagcggtata aggagcgcag attcgcagcc gttagatgca agtaagagta
     2161 ataacgggtc gcgttgccag agaaacagat cgtctgcacg gtgcgtcaag gtcggttgtc
     2221 gggtttgtag cgtttgtgtc agcaatacac tgtcgccagg catattgagt ggcagacacg
     2281 cacaatgtga ggcagaaaga tcgctgcacc acaggctaaa cttctcgttc tttcgatgat
     2341 cgcccagtac catactgact gaagccagac cgaaaaaatg atggatctca cgagcgacgt
     2401 cagcgatcag atcgtcgaga tcaagatgtg acagcactgc attagtaata tcaaccagta
     2461 cccgatgatg atcgcgatct ttctccagca aacgcagcgc cacctgctgt tcttcatcat
     2521 tgagccatga ttgcagaacc ggggtgatta tccgcgccag ccagttaaac agtgtgcaat
     2581 cctggggagt aaaacgactg ccgtgggtgc gaataaagcg tagctccgcc attccactac
     2641 tgccgagaag gacggcgtga cgcgaataat cgaccatctc gccctgatac tctgttgttg
     2701 ccgtcacatc gctgccatca tcatggtagc ggtaatagaa gttgcttttg agaacatgca
     2761 gttcaataag ttgtaccggg ctgaacggcg gcggcagcgg gatgaaggcg cgtaataaag
     2821 acgccttacc gtgtttctgc gctaaccgct ccgcgagcat tccgtttacc gcttcaattg
     2881 ttattccttg cggcggggca aacatcgcct cgtctgacat agccatcaac agtccttatt
     2941 aatgagaaat ccttctcacg taatttttct ggccatcaga tagatggccg gttcctgcac
     3001 aatttcatcc aacagatgaa tgaattgaat gctacgcgcc tgccacacag acgtcgcgtc
     3061 atccgccagt ggcgcgaatg cccgggtcaa cagggcggtg tggctggcgt caataacaat
     3121 ctcaccaaag gtcatcaccc ccagcatctt acgtcgggct tgctcctctt cgaccagtgc
     3181 aagccaatct tcgtattccg tcagtgggca gcggaaggcg acattcagac aatcaatcac
     3241 gccaacgtga tgaccgatag ccagcgagta atacattacc tgagagctgt gttccggcat
     3301 ctcgtcgcta ctggcgacaa acttttttcg cagcgtccag aaaacaattt ccccgcactc
     3361 ttcagtcatg taacccccgg agtacgcgaa agagttgctg aacgatacta ttgagacgtg
     3421 gatcgtcggc atcgcgcaac caggtgttaa cccgatttcc tgtaggatcg tcgcttaaat
     3481 ggcgcaggag ccgatcgcaa atttctcgcc cctgacgata gcccgccagc cgccgcgctt
     3541 ctcgctcaat ggcgatacgc tgtgatggcg ggatctgcgg ccacaacggt tgcatagtca
     3601 ccccggtggg atcgcgataa tccaccgcgt gaatcttctg ttgcagcaaa ccgagcgcca
     3661 cggcgaaacc gtgaatggtg gcggccggtg ttggcgggca gccggggatc caaacatcaa
     3721 tggggacaat ggtgtcgctg ccgccccaga cgctgtagag atcgtggaaa ataccgccgc
     3781 cgacaccgca cgcgccgtag gaaacacaga ttttatgatc gggggcagac tcatacgccc
     3841 gaagtgcagg catacgcatc gcccgggtga ctgcgcctgt aaataacaaa atatcggcgt
     3901 gacgcggtga tgaaataacc ttaatgccaa aacgttctgc gtcgaatact ggtgtgatgg
     3961 cggcaaaaat ttcgatttca caggcgttgc agccgccgca atcgacgcga taaacgtaag
     4021 ccgagcgacg gatatcctgt agcaaatgct gcttcatctt ttgcgtttgc tcgtccagcg
     4081 tgatgggctg gctgacatgt tgtgtaagca ctgggctcat agctgctcct tagccaccag
     4141 cggtacatct gtatcgtcgt tgagcagcgt cgcgcgttgt ttgcactccg ggcagacgct
     4201 cgcttgcgcc cataacattt cgcggttttg tggcgcattt tgttgctgtg ccaacaattc
     4261 agtagccagt gcgacggttt tttgctgggc aaacgggcgt tcgcaacggc tgcaacgttg
     4321 tagatggaac gtcgcgcggg tatagagatc ggctttattg gtgacggtca gttcaaagtt
     4381 attggtaagc tggatggctc tggtcgggca cacttcttca caacgtccgc agtaaataca
     4441 acgcctcaga tagagctgcc aggtgcgcga attttgctgg tcgtcggtct ggatagtcag
     4501 cgcatttgcc ggacaagcac aggcgccgca ggcaatacat tggctgggca tcaggtccgg
     4561 tttcccgcga aagcctgggc tgacctccag tggcgcgaag ggatatttca ccgtcgcggt
     4621 tccggcgcgc ataatagttt tcagtaactt cagcatcttc tgtccttact tcagcggcga
     4681 acggttacga tcaatgccgt agcgttcgat ctctttatac ggcacggttt ttgactggcg
     4741 cttgcgcaca tctaccagcg tcacacggtc ggtacaggag tagcagggat caaggctacc
     4801 gataatcagc ggtgcgtcag aaacggtatt gccgcgcagc atgtaacgca acaccggcca
     4861 gttggcgtag gtggcggcac ggcagcgcca gcggaacaat ttttggttat caccgagcat
     4921 gctccagtgc acgtcttcac cgcgtggcgc ttcaacaaag cccagcgcga atgcgtgagg
     4981 tttatagcta aagccttcgg tcagcagtgg ggtatccggc atgttgtcga gggcaaattc
     5041 cagcattgcc agcgaatcaa acgtctcttt gacacggacc atcacgcggg agaaaacatc
     5101 gccgccggta aaggtaaaca gtgtttttgg aatattgccg tagtcggcgt aggggtgatc
     5161 aaagcgcaaa tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc
     5221 catattcggc gtagcaagca gcatctctac cagctccgac acgtcggcgc gcatctcgcg
     5281 caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat
     5341 cagattcaga ccgtaggttt tacgcgaccc gatcagcaat tccgccatcg tcatcgactt
     5401 ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaaa ccaacgaaat ggcaggagag
     5461 gccaaggtta agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg agcgaatagt
     5521 atgtgctcgt tgcggcacct caatccccag tgcattttca accgaattgg tataggccac
     5581 actgtgggca aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta
     5641 gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca ggcggtaatc
     5701 agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt catcggaggt
     5761 gatatgcagc gggccgacag ggatcacccg cgcatcgctg ttgccctcat tgataaacgg
     5821 atacgtttcg gaatcagtcg tcggttcagg gcgcagtcga taatccatcg catctttgcg
     5881 cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag
     5941 gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga ccgcggcagg
     6001 gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagcg ccttcaccac
     6061 aatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt aatgaccgtt
     6121 aagtgtccgc tcgtcgttgc caaacaggac cggaagccag ccatcatgtt gataataaag
     6181 atactgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaggcgt
     6241 ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc
     6301 ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc
     6361 ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg cagatcgtgc gtccctgaga
     6421 ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata tgcgttccca
     6481 tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccgagatcac
     6541 cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc
     6601 ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg tgattacgtg
     6661 ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg
     6721 ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc
     6781 cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga
     6841 acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca aaaattccca
     6901 gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg ctggagtacg
     6961 ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca
     7021 acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct tggcaaataa
     7081 tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagcaggg
     7141 cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata
     7201 gcccggtttt ggtaccaaag ccaattagca aaaacacaaa ggccaacagc attaatgttg
     7261 ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg
     7321 gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac
     7381 caacagtaca aataatgatg tacttccatg cagcttccag cgatgaacgc tgaccgtaaa
     7441 tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca
     7501 ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt
     7561 aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc atgtagccaa
     7621 tcgagtaaat accggtgaga aagccaatca caccaagaat ggcgaggaac aaaccgccca
     7681 gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca gcggcagttt
     7741 ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt aatacggtca
     7801 ccgtgcgagt cgcagaaagt ctccgtttcc ggcaggcaaa acagagcagc gaaaaaagca
     7861 gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc
     7921 gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac
     7981 accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac cagttccggt
     8041 gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca
     8101 aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag cagaaaatga
     8161 cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt cacgggctga
     8221 acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg
     8281 aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac
     8341 ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg
     8401 gcgaacaggc acgagagggt ggcgaaaata gacaccagca ccagtgactg gcaggcgtaa
     8461 aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagtgatgt cagcatcatc
     8521 agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc
     8581 aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc aacaccaggc
     8641 gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg ccaggcacga
     8701 cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc atcagcagaa
     8761 tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccggca gcaaacagcg
     8821 ggaatttact gaagaagccg ttgaacggcg gcacgccggt aattgccagc gctgccacgc
     8881 agaaacccac gccaggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc
     8941 gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg
     9001 cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg aagatggaga
     9061 gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct
     9121 tcatatcctg ctgtggcaaa tacatcagaa atccataaag aatggtgacc agggccatga
     9181 ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa
     9241 aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg
     9301 gcgcttccat tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg
     9361 cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac tcgctcattg
     9421 cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca
     9481 acgagccgat atgagtgata agcaacgctt ttagtgccga acgctgcgct ttatcgctct
     9541 ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac
     9601 cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc agcaaaaatg
     9661 cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg
     9721 tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtactca
     9781 cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga
     9841 cggcaacttt atcggcctga taaaacgtgg agatcagcga caacatgcac agcgtggtca
     9901 gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg aaacgaccag
     9961 tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat tccgtcagtg
    10021 ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg aagccaagcc
    10081 aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct gcgattgagg
    10141 ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc agcaggcagg
    10201 cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac agtgctgcca
    10261 tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca ccggaatatt
    10321 cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag ggaatttttc
    10381 ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg gttagcggcg
    10441 agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa cctgctatca
    10501 gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaataccg agcgtcaact
    10561 cgcgactggc accgactccc gcaaacgggc ttccggtatc cagcccggaa agagcaaaga
    10621 aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg cccgcaaaag
    10681 gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg ctgattaata
    10741 cccacggcat caggcggaac atcagacctg aagatgtcgg cgcaacttcc tggcgtttaa
    10801 acagtttgtg gatatcgcga taatcctgcc agatccccgg tccgcggcgg gagtgcattc
    10861 gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc agaatcacgg
    10921 cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc atggtgattt
    10981 ccttagacgg caatagcgat tagcagcaca accagtgcgg caaccacata caggcaatag
    11041 agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg ggtggcgctc
    11101 acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc cctggcggtg
    11161 acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg tagacgaaat
    11221 agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg ggccatcgca
    11281 ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc tgcgcggcgc
    11341 gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag taacagcaac
    11401 agtaatgagg gagtgacccg tgtatgaaac gtgccgggta caagtgctat tccgctggcg
    11461 acagtgacgg gaggggtatc ggtaaaggca tgggcaatat gcattatctt cggtgccagc
    11521 caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat ggcgacgatc
    11581 atcgtatttg gcacttcctg tgcctcttca gcgtgtgtac tgcgcggcgc accacagaaa
    11641 gtaataccgt acattttgac gaagcacatt actgccagcc caccagtgac tgccagcatc
    11701 accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact tagtgagaac
    11761 agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaag ggctgatatc
    11821 gcgaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc cagtgccccc
    11881 attttttcca tgtcgtgggt atgcaaaagc gaaataatcg cgcccgcgcc gagaaatagc
    11941 agccctttga acagcgcatg gttgagcaga tgaaacagtg cgccgagcag tccaaccacg
    12001 gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgactgc cagcaaaata
    12061 atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg ttccgccagc
    12121 gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag aatgccccac
    12181 cacagcggca aacccgtttg cgccagcaga tccatcgcta ctttcaggat gccgaaaata
    12241 ccgattttga ccattacgcc agacatcaac gccgaagcgt gcgatggtgc ggcagggtga
    12301 gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa accgaaaaag
    12361 gccagcagaa acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg aaactggcaa
    12421 aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcataatc agcaccgatc
    12481 cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta tcctgcctgg
    12541 caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg aaccaaaaag
    12601 cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aagattcata aagaagccca
    12661 tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag agcgaacaaa
    12721 ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc attcgcaggg
    12781 ttaaatcggc aaagtcaaaa ggggtggcaa aatgggcgac taaagttaca ccagcatgta
    12841 attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg atgccggaaa
    12901 gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg gcaaacagat
    12961 agaggatcag cgaccaggtt aataattgca gggcatccat tagtgttgct cctgagtgag
    13021 ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct gtttttcttt
    13081 catctgacgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg ggcaagcgcg
    13141 aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg cgacggtctg
    13201 gacacccggt tcccagcgca gcaacgcatg ttggggcagc acgttttctt cgccgtcttt
    13261 gagtgagcct tcagcctgaa aaacatattg cgcatgggca ttcaccggac ggcttcctga
    13321 agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa tacagaggct
    13381 ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc agacctgcag
    13441 gcaaggggct tcctcacagt gatgatacac gacaggggca gtgattgttg acgtcttcgc
    13501 cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg aacaggcagc
    13561 gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc ggttcatgtc
    13621 ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat gccattctga
    13681 ttaactcaat atattcaata agttatgtat tgactggatt ctgaacggta caaaaatgat
    13741 ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga aagcgagaat
    13801 gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc acggaatgga
    13861 gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt gattgctggt
    13921 tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga agctgatgcg
    13981 atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc cgaattgttc
    14041 gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt tttccgcaaa
    14101 acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc ccgctttttt
    14161 caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg gggtcatggc
    14221 tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat taacttgttc
    14281 tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca gtggattcat
    14341 tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat actgccttgt
    14401 gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc gtccgcagat
    14461 gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt ttcagcaggt
    14521 tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag
    14581 cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga tgctggcatt
    14641 ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag ttcggcacct
    14701 ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga aagcagcata
    14761 ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt acagccgcaa
    14821 ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc caacgcagtg
    14881 atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag gggtgagttg
    14941 ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc tgtctgcttg
    15001 cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg atggcccatg
    15061 ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg
    15121 gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc gatcgtttct
    15181 gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga tgtggtgatg
    15241 atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg cgctaacgct
    15301 actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt cggctgcctg
    15361 acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc
    15421 atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg tactggctgc
    15481 gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa
    15541 gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc agatgatttt
    15601 gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattggg cggtcatggg
    15661 gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatatg caaactggca
    15721 gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc attacacaac
    15781 aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga actgggtctt
    15841 gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg tcgtgagacg
    15901 gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt tgatgactta
    15961 ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc
    16021 tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag cctacctgga
    16081 agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg tgacctccgg
    16141 tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg
    16201 tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca cgggcgatac
    16261 cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg tggttagcgt
    16321 gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga cactgaccac
    16381 cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc gttatcaaat
    16441 ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgctga acctggaaca
    16501 ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcatgg agatgatgaa
    16561 cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg
    16621 tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac caatgctggt
    16681 cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg aaaaagtggg
    16741 catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt ataagccgct
    16801 gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat ccggtgacta
    16861 taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg atccgaaagg
    16921 tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg ctgcgtttag
    16981 caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat acgcaaacgt
    17041 gtgcgtctgc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt ttgtcggacg
    17101 tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc agacagcaaa
    17161 agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca agctgagttg
    17221 tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa
    17281 ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga tcgcaaaggt
    17341 atggtgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc gggtatcccg
    17401 actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg
    17461 ccggttgagt gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg tcttggaatc
    17521 gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg
    17581 cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag caaagagaac
    17641 ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat
    17701 gatgctggtc tgattctggt cgacttcaag ctggaatttg gtctgtacaa aggcgaagtg
    17761 gtactgggtg atgagttctc cccggacggc agccgcctgt gggacaaaga aacgctggag
    17821 aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaggc ctatgaagcc
    17881 gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc ttgccgtgct
    17941 gctggcacgg caagacaacc attctcgtaa gatgtgcatt gaacgctcat tcctccgcca
    18001 tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat ttctataatg
    18061 aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac aatgttgaag
    18121 acaggcgcaa cagctctggt ggtccgtcta tgggcggtcc cggttttcgc ctgccgagcg
    18181 gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat ggcgttgatt
    18241 taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacgcgg tcaattagcc
    18301 caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaactacg gaagacacct
    18361 ggggacagca gttcgagaag atgggtaaaa cctatcagca accgaagctg gtcatgtacc
    18421 gtggaatgac gcgtaccggc tgcggggcgg gccagtccat aatggggcca ttctattgcc
    18481 cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa gacaaacttg
    18541 gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt catcatgtgc
    18601 agaaactgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg acgcaggcgg
    18661 aagtgaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc ggtgtctggg
    18721 ggcatagtat gcagcagcaa ggcgttctgg aaaccggcga tctggaagag gcgctgaacg
    18781 cggcgcaggc catcggcgat gaccgtttac aacagcaaag tcaggggcga gtagtaccag
    18841 acagtttcac tcatggcact tctcagcaac gctacagctg gtttaaacgt ggtttcgaca
    18901 gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat ttaacttccg gggcagggat
    18961 ggctgaactg actgcgcttc acacattaac agcgcaaatg aaacgtgaag ggatccgccg
    19021 cttgctggtg ttgagcgggg aagagggttg gtgttttgat catgcgctta agttacgtga
    19081 tgccttacct ggcgactggc tgtggatttc gccgcagcca gatgctgaaa accactgttc
    19141 tccctcggca ctacaaactt tacttgggcg cgagttccgg catgcggtat tcgacgcccg
    19201 ccacggcttt gatgccgctg cctttgcagc acttagcgga acgttgaaag cgggaagctg
    19261 gctggttttg ttactccctg tatgggaaga gtgggaaaac caacctgatg ccgactcgct
    19321 gcgctggagt gattgccctg accctattgc gacgccgcat tttgtccagc atctcaaacg
    19381 cgtacttacg gcggataacg acgctatcct ctggcggcaa aaccagccgt tctcgttggc
    19441 gcattttact ccccgtactg actggcaccc cgcgaccggc gcaccacaac cagaacaaca
    19501 gcaactctta cagcagctac tgaccatgcc gccgggcgtg gcagcggtaa cggctgcgcg
    19561 tgggcgcggt aagtcggcgc tggcagggca actcatttct cgtattgcgg gcagtgcgat
    19621 tgtcaccgcg cccgcaaaag cggcaacgga tgtactggca caatttgcgg gcgagaagtt
    19681 tcgctttatt gcgccggatg ccttgttagc cagcgatgag caagccgact ggctggtggt
    19741 cgacgaagcc gcagccatac ctgcgccgtt gttgcatcaa ctggtatcgc gttttcctcg
    19801 aacgttgtta accactacgg tgcagggcta cgaaggcacc ggacgtggtt ttttgctgaa
    19861 attttgcgct cgctttccgc atttacaccg ttttgaactg caacagccga tccgctgggc
    19921 acagggatgc ccgctggaaa aaatggttag tgaggcactg gtttttgacg atgaaaactt
    19981 cacccataca ccacaaggca atatcgtcat ttccgcattt gaacaaacgt tatggcgaag
    20041 cgagccagaa acgccgttaa aggtttatca gttattgtct ggtgcgcact accggacttc
    20101 gccgctggat ttacgccgga tgatggatgc accagggcaa tattttttac aggcggctgg
    20161 cgaaaacgag attgccggag cgctgtggct ggtggatgag gggggattat ctcaacaact
    20221 cagtcaggcg gtatgggcag gttttcgtcg cccgcggggt aatctggtgg cccagtcgct
    20281 ggcggcgcac ggtagcaatc cactggcagc gacattgcgt ggacggcggg tcagccggat
    20341 agcagttcat ccggcgcgtc agcgggaagg cacagggcag caacttattg ctggtgcttt
    20401 gcaatatacg cgtgacctcg actatctttc ggtgagtttt ggttacaccg gggagttatg
    20461 gcgtttctgg caccgctgcg gttttgtgct ggtgcggatg ggtaatcatc gggaagccag
    20521 cagcggttgc tatacggcga tggcgctgtt accgatgagt gatgcgggta aacagctggc
    20581 tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct ctcgcgcagt ggaatggcga
    20641 aacgcttcct gttgatccac taaacgatgc catcctttct gacgacgact ggcttgaact
    20701 ggccggtttt gctttcgctc atcgtccgct attaacatcg ttaggttgct tattgcgtct
    20761 gctacaaacc agtgaactgg cattaccggc gctgcgtggg cgtttacaga aaaacgccag
    20821 tgacgcgcag ttatgtacca cacttaaact ttcaggccgt aagatgttac tggttcgcca
    20881 gcgggaagag gccgcgcagg cgctgttcgc acttaataat gttcgcactg agcgtttgcg
    20941 cgatcgcata acgcaatggc aattttttca ctgactcctt caattttctc ccatggtcat
    21001 tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga gttcgccatg aaacatgacc
    21061 attttgttgt tcaaagcccg gataaacctg cgcaacagtt gttactgctt ttccacggtg
    21121 tcggggataa cccggtggca atgggggaaa taggcagctg gtttgcgccg ctatttcctg
    21181 atgcgctggt ggtgagtgtc ggaggcgcgg agccgagcgg taatccggcg gggcgtcagt
    21241 ggttttcggt gcagggtatt acggaagata atcgccaggc gcgtgtggat gccatcatgc
    21301 cgacgtttat tgaaacggtg cgctactggc agaaacagag cggagtaggg gctaacgcca
    21361 cggcgctaat cggtttctct caaggcgcga ttatggcgct ggagagcatt aaagccgaac
    21421 cgggccttgc ttcacgcgtc attgctttta acggacgcta tgcgagcctg ccggaaaccg
    21481 cgtctacagc cacgacaatt cacttgattc atggtggtga agatccggtt atcgatctgg
    21541 cgcatgcagt tgcggcgcag gaagcgttaa tcagtgccgg tggtgacgtg acgctggata
    21601 tcgtggagga tttaggccat gcgatcgaca atcgcagtat gcaatttgcc ctggatcact
    21661 tgcgttatac cattccgaaa cattatttcg acgaagcgct aagtggcggt aagcctggcg
    21721 atgatgatgt gattgaaatg atgtaaaaga cagattacct ggcctgataa gacgcgtgag
    21781 cgtcgcatca ggcatcgttc accattgccg gatgcggcgt taacgcctta tccggcctac
    21841 ggttgagcac tcagcttact tcttcggttg atcctttttc ggccagtcgt cgtcatcgtc
    21901 ccacttatcg ttgaaatcac gatgcggggg aagctccggc ttgttggcga gaaatttctt
    21961 atggtcgacg cgcttgagat ctttaatcac gttcagcagg acgcctacca aaaagacaat
    22021 caccagaatc caccaatatt tagccagcca gtccatgctt atttcctctt gcagaaccac
    22081 tcatcaggcg acgagctgtt ccatgatacg ttgatacata cgggcaagta gctgcaggtc
    22141 ggcagcgttc acacattcat taattttatg aatagtggca ttgaccggcc cgagttccac
    22201 cacctgcgcc cccatgcgag caataaagcg cccgtcggac gttccgcctg tggtcagtag
    22261 ctgcggttta atttcattat agtgctcaac cgcgttaacg accgcatcca ccagtttacc
    22321 gcgcgcggtc aaaaatggct gcccggaaag ccaccaatcc accgtatagc gcagttgatg
    22381 tttttcaagc agggcaagca cctgcgcttt gatcatctca tcagtcagtt cggtgctgaa
    22441 gcggaagtta aactgcacaa acagttcacc cggaataaca ttgttactgc ccgttcccgc
    22501 ctgaatatta gcaatctgca tactggtcgc cgggaaaaat tcattgccct gatcccactc
    22561 aatagccact aattcattaa ggaaaggtgc tgcgcgatgt actggattgt cagccagatg
    22621 cgggtaggcc acatgcccct gaacgccatg aatggtaagg ttgcaggtta acgatccgcg
    22681 acgaccattt ttcaccacat caccaaccac ttcgatactc gacggttcgc cgaccaggca
    22741 gtaatcgaga cgctcattac gtgccatcaa cgcttcgacg acttttaccg taccgttgtg
    22801 ggcactggct tcttcatcag aggtgatcag aaatgccagt cgccccgtat ggttgggatg
    22861 ttgtgcgaca aagcgttctg ccgctaccac catcgccgcc agcgagcctt tcatatctgc
    22921 cgcaccgcgc ccgaataaca tgccatcacg aatggtgggt tcaaacggcg gattgatcca
    22981 acgatcggcg tcgccaggcg gcaccacgtc ggtatgcccg gcaaaggcta acgtttcacc
    23041 ttgcccacgc catgcccaaa aattctgcgt atcggcaaaa tccatgcgtt caacggtaaa
    23101 accgatcgcc tgcaaacgtt caatcaacaa agcctgacat cctgcgtcat caggactcag
    23161 ggaagggcgg cgaataagct gttgtgtcag ctcaataacc gggcacgaca tagactacac
    23221 ctcatggaaa aattgctgat aactggaatc actgaaaccc agcagcatag gcttaccagg
    23281 cgcgcagagc aatggacgtt tgataattgc aggcatttca gtcattaatg ccgccgcaga
    23341 ggccgcatcg gtgattttat tgcgggtggt ttcgtccagt ttacgccagg ttgtaccacg
    23401 ggtgttgagt aacgcttccc agcctaattc gttgataaaa ccgttcaata attcgctgtc
    23461 cagcccatcg acgcggtaat catgaaaacg atagtcgatg ttattggctt ccagccaacg
    23521 gcgagccttt ttaatggtgt cacaattttt gatgccgtaa agtgtaacca taatgaatcc
    23581 tttttcgatt tatgagaata aatactcatt taagagtgaa aacattttgc ctgcaaatga
    23641 ttatcaggaa atataacatt atcgaagtat tcaaattaac aactattaaa taatattaaa
    23701 catttttaac gtaaacatta agaatattca tcttgttatc tgtctgtatt ttaaccagaa
    23761 ttaaatcagt attagatgcc gtatgaatat ctcgaaatgt tgctgtgtat cacatacaaa
    23821 tcatcaccgc aggaccatag tatttacata aggtttgtcc cccggaggta gttatcgcag
    23881 caatcgagta aattagcttc accacggtat attgtttccg tagaataccc ataattataa
    23941 gagaggttgt tatgattgaa cgtgaactgg ggaactggaa agactttatt gaagttatgc
    24001 ttcgtaagta attcctgaga caaagtacaa acagcaagaa cctgctgagc aagttcttaa
    24061 tcggaaagtt gaaaaaggcg acacattggc atgtcgcctt ttttattgct tattccgggc
    24121 gcggcttcag cgggaagcgg cggcgcacca gcacaaagaa cagcggcacg aagtaaatag
    24181 ccagaatagt agccgaaatc atcccgccca ttacgccagt acccaccgca tgctgaccac
    24241 cggaaccggc acccgtgctg gttgccattg gcaatacgcc gaagataaat gccagcgagg
    24301 tcatcagaat cgggcgtaaa cgctgacggc aggcgtggag cgtcgcttca aacaggtcgt
    24361 ggcctttttg gttcatctcg ttagcaaact cgacgatcag gatggcgttt ttcgccgata
    24421 aaccaatgac cgttaacagg cccacctgga agtaaacgtc gttttccagc ctccgcatcc
    24481 aggttgccag cagcgcgccg ataaccccca gcggcacgac cagcattacc gagaacggta
    24541 ccgaccagct ttcatacaat gcagccagac acaggaatac caccagcaag gaaatagcgt
    24601 acagcgccgg agcctgcgcg ccggaaagcc gctcctgata cgacatcgcc gtccactcca
    24661 gaccaaagcc gtttggcaac tgcttcacta acgattccat aatatccatc gtcgtaccgg
    24721 tactgacccc cggtgcggct tccccaacaa tctcaaccgc agaataaccg ttatagcgtt
    24781 ccagacgcgg tgagcctgtc tcccagcgtg aggtcgcgaa agcagagaag ggcaccatgc
    24841 cgccatcttt atttcggaca taccagagat tgatgtcatc tggcagcatg cgatacggcg
    24901 cagctgcctg cacatagact ttcttcacgc gaccgcgatc cataaagtca ttcacatagc
    24961 tcgaacccca ggcggtttgc agtgtgtcgt taatatcgtc gatagcaacg cccagcgcct
    25021 gagctttacg ctggtcgata tcaatctgca actgcggact gtcatcgagg ccgttatggc
    25081 gcacacgggt tagctccggg ttttccgccg ccagcgccag caactggtta cgtgctgcca
    25141 tcagcgcatc gtgacctgct ccagcgtggt cctgcaactc catatcaaaa cctgcagaac
    25201 taccaagacc gctaattgct ggcgggctgc tggcgataac gcgagcttct ttaatttggt
    25261 taaacgcctt tgttgcacgc tcgataatgg caaacgaggt gccggtctta ctgtcgcgtt
    25321 cgctccagtc tttcaggcgg ataaacattc gcgccacgtt ttgcccgtta cccccagggc
    25381 cagaaccaac agtggcaaac accgacatga tgttgtcttt ttcatgggtg aagtaatatt
    25441 tctcgatttg ctcaacgact ttcagggtct gttgttgggt tgaaccgctg ggcaattgta
    25501 ccgaggtagt aaacatgcca cggtcttcca gcggtaagaa cgacgtcggc aaacgcagga
    25561 acaggaacac catgccgcca agcagcagga cataaatcac aatccagcgc aggctgcggt
    25621 ggagaatttt cgccacccct ttttcgtagc gttcggcgtt gcggttaaac atctggttaa
    25681 accaggcaaa aaagcctttt tgcccgtgat gctcaccttt tttcagtggc ttaagtagtg
    25741 tggcacacag agccggagtg aggatcatcg ctaccagtac tgacagcacc atcgccgcaa
    25801 caatggtaat agagaactgg cgatagatgg caccggtggt gccgccgaag aaggccattg
    25861 gtacaaatac cgccgaaagc accatcgcaa tcccgaccag tgccccctgg atctgcccca
    25921 tcgatttacg tgtggcttcg cgaggagtga gtccttcctc actcataata cgttcgacgt
    25981 tttccaccac cacgatggcg tcatccacca gcagaccgat cgccagcacc atcgcgaaca
    26041 tggttaaggt gttgacgctg taaccgaagg cgtaaagtac ggagaaggtt cccatcaaca
    26101 ccaccggcac ggcgatagtg ggtatcagcg tggcgcggaa gttttgcagg aacagataca
    26161 taacgaggaa aaccagagcg atagcttcca gcagcgtttt caccacgtct tcaatggagg
    26221 ctttaacaaa cgaggtggtt tcatacgcca ccttgtattc cagtccatgc gggaaatact
    26281 gcgccagctc gtcgagacga ttgagcacca gctccgctgt cgccatttcg ttagcgccgg
    26341 aggccagttt tacccccagc ccggaggctg gcttaccatt gaagcggcta agataatcgt
    26401 atttctccgc ccccatttcg acggtggcga catcgcccag ccttacctct gagccgtcct
    26461 gattgacccg caaggtgata tcgcggaact gttctggtgt ttgcagcagt gactgggcgt
    26521 taatggtggc gttgagcgcc tgcttatcga cggaaggtgt accaccaagt tgcccaaccg
    26581 caatctgcgc gttctgtgac tcaatggcat cagtgacatc tttagccgtc atctggaaac
    26641 tg
//
LOCUS       DACAPB010000048        26455 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.048, whole genome shotgun sequence.
ACCESSION   DACAPB010000048 DACAPB010000000
VERSION     DACAPB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.048"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..687)
                     /locus_tag="IT904_001806"
     CDS             complement(<1..687)
                     /locus_tag="IT904_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP3191599.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDK"
     gene            complement(898..2295)
                     /gene="ccp"
                     /locus_tag="IT904_001807"
     CDS             complement(898..2295)
                     /gene="ccp"
                     /locus_tag="IT904_001807"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAP3191600.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQEQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2700..4349
                     /gene="treF"
                     /locus_tag="IT904_001808"
     CDS             2700..4349
                     /gene="treF"
                     /locus_tag="IT904_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAP3191601.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4400..5002)
                     /locus_tag="IT904_001809"
     CDS             complement(4400..5002)
                     /locus_tag="IT904_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP3191602.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5450..6421
                     /locus_tag="IT904_001810"
     CDS             5450..6421
                     /locus_tag="IT904_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191603.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEYLD"
     gene            6470..7483
                     /gene="yhjD"
                     /locus_tag="IT904_001811"
     CDS             6470..7483
                     /gene="yhjD"
                     /locus_tag="IT904_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAP3191604.1"
                     /translation="MTQENEIKRPIQDLEHEPIKPLDNSEKGSKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7894..9216
                     /locus_tag="IT904_001812"
     CDS             7894..9216
                     /locus_tag="IT904_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP3191605.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9398..11458)
                     /locus_tag="IT904_001813"
     CDS             complement(9398..11458)
                     /locus_tag="IT904_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP3191606.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11528..12295)
                     /gene="pdeH"
                     /locus_tag="IT904_001814"
     CDS             complement(11528..12295)
                     /gene="pdeH"
                     /locus_tag="IT904_001814"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAP3191607.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLCFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12527..13456
                     /locus_tag="IT904_001815"
     CDS             12527..13456
                     /locus_tag="IT904_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HAP3191608.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13552..15048)
                     /locus_tag="IT904_001816"
     CDS             complement(13552..15048)
                     /locus_tag="IT904_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP3191609.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAVWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15269..16555)
                     /locus_tag="IT904_001817"
     CDS             complement(15269..16555)
                     /locus_tag="IT904_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAP3191610.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16738..18726)
                     /gene="hmsP"
                     /locus_tag="IT904_001818"
     CDS             complement(16738..18726)
                     /gene="hmsP"
                     /locus_tag="IT904_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAP3191611.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRAYVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAKELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPGDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18808..22281)
                     /gene="bcsC"
                     /locus_tag="IT904_001819"
     CDS             complement(18808..22281)
                     /gene="bcsC"
                     /locus_tag="IT904_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HAP3191612.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPRAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22263..23369)
                     /gene="bcsZ"
                     /locus_tag="IT904_001820"
     CDS             complement(22263..23369)
                     /gene="bcsZ"
                     /locus_tag="IT904_001820"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAP3191613.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKEGLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23376..25715)
                     /gene="bcsB"
                     /locus_tag="IT904_001821"
     CDS             complement(23376..25715)
                     /gene="bcsB"
                     /locus_tag="IT904_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAP3191614.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25726..>26455)
                     /locus_tag="IT904_001822"
     CDS             complement(25726..>26455)
                     /locus_tag="IT904_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191615.1"
                     /translation="VDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVW
                     VFYNLIVLGGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIK
                     INGQAQILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTF
                     ARADTWALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWV
                     VSFIPRRPERSETAQPSDQALAQQ"
BASE COUNT         6223 a         7272 c         6624 g         6336 t
ORIGIN      
        1 tttatccagc gcatcgtgca gcggttgtgg gaactcatag ttaccggtgt aggtcacgcc
       61 gaaagtcggc accacgccga tggtgttttc gtcacaggct tcaatcatgc gtttcgggtc
      121 cataaacaac tgaccggggc gcatagggat ctcacgcagc tccacatccc agtagcgggc
      181 gaatttatgc cagcagattt gtaccggacc gcacaccagg tttggtttat ccgttggttt
      241 gcctgcagct tccatacgct tgcgccaacg ccatttcatc gccatcccgc cgagcataca
      301 ggcctcggaa gaaccaatgg tgttggtgcc aacggcctga ccatttttcg gcgcaggcgc
      361 atgccacaga tcggcaacca tatttacgca acgcaggtcg atggctgcgg attgcggata
      421 ttcttctttg tcgatccagt ttttattgat cgacaaatcc atcaatttat ggacgttttc
      481 gtcgtcccag gtctggcaga aagtggccag gttctgacga gcgttgccat caagatataa
      541 ttcatcattg ataatctgga atgcgacatc atcgcgcatt tcgtgcagcg gaaatcgttt
      601 tgactccgcg atagtagaaa tggcctttgc gccaaaacgt gaatcgagta gttctgagcg
      661 gaaatccgtt aacagcttct ggtccatttc gaactcctta aatttatttg aaggcaataa
      721 aaaagtagga tttatccgca atggaagcaa ggcattacag gcttaattta aataacaaaa
      781 tcctaagcag aaaaacgact ttttataata taaacattta acatgataat attaaaaaca
      841 gactttatta tcgtaataaa aatcgcccaa acattcctgt tcgggcgatc aaattaatta
      901 ttgtttatcc tgcatatacg gcgtgtacac cccgttcaga ctgtgcagga aagctacgat
      961 atcatccaca tcctcctgcg gcagctcttt gcctacctga tagcgcagca tcagtttcac
     1021 cgccccgtcc agcgtcggca cgtcaccgcg atggaagtac ggtgccgtta aagcaacgtt
     1081 acgtaaaccg ggtactttct gacgcaattt atcacgctct tctttagtca cattcatacg
     1141 accaatatcc gccgccgtaa tttccccaaa gttaaagtct tttttcagcc ccaacggttc
     1201 aaaggaacgt ccaccgagaa taataccacc atgacaagtt gcacatttat tatctttaaa
     1261 taattgatag ccttttttct gttgcgccgt cagcgcattt tcatctccac gcaaccattt
     1321 atcaaatggg gaatccggcg taattaatgt tttctcaaat tcagcaatgg catcagtaat
     1381 attttcgcca ctgaaacctt gcggatagac gccgaggaac tgcgctttaa gctgcggatc
     1441 tttttccagc ttagcaataa tttcgtccca ggatttcgac gccatttcaa tcgggttcaa
     1501 cggcggtcca ccagcctgct cctgcaatgt tgccgcacga ccatcccaga actgctcaac
     1561 gttaaatact gagttaaata ccgtcggcgc gttaatcggc ccaactgcgc caccaacacc
     1621 aatcgatgtt tttctgccat cgacgccccc cgcattcaac gcatggcaat gagcgcatga
     1681 aatggtgcta tcagccgata aacggggatc gtgatacagc gcaaagccca acgccacttt
     1741 ttgcgcatcg gtaggcagtt tttgcgggat gggctgcacc ggttcattgc gatgctccgg
     1801 agcagtatca ttgctggcgt aatattccgc gcgctgtttt gcaatccagg ccagtatttc
     1861 cgcccgctct tcatcactca ccttacccgc ccagtgtagc gcggtataac gcgttggtgg
     1921 catagtttca tactgcatca cccattcaat cttattcaga tcgctttgcg aaacgggttt
     1981 atcagccagc agtgccgcac gcacggcctc gaggttaaaa gatttatatc caagcttaat
     2041 gtcgtaatcc atcaactgtt tcgcgccagg aatataataa taggcgggta attctgccga
     2101 aggcgtgtgg caatagtcgc atcctttttc gcggagaaag cctaaaacct tattattttc
     2161 actgacagca gatgcctgaa catcggcctg tttactgcgt ttattatcgt ggtaccacac
     2221 ataaccagat aaccctaaat agcaaatcgc gacgccagcc aggccgatcg cggtaatacg
     2281 tgagaccatt ttcattattc ttccctcacg gtggtgagtt attgtgaccc agaaaatttt
     2341 ccagaatgct gcaaacgtct ctgcgagaca aagccatcat aaaaaacagt gagatacgct
     2401 ttttgatagg aatcaatcaa ttacatgaat ataaactatc aataagatag cctgtgccta
     2461 ttacccagat caggaatggc atatcatgat tgcggcgaga gcaggattga atgttctgaa
     2521 aatgaaaaac aacacgaaat ccgtattttt ataaattcca tggatattag tgcgttatat
     2581 tttttgacag gttaataaac gtgatccacc gcacgctttg tcgcccacca ggcggagcga
     2641 atgactaccc ttaaagaaaa gcccgataat tagcgacgaa tttcggaggt tggatcctta
     2701 tgctcaatca gaaaattcaa aaccctaatc cagacgaact gatgatcgaa gtcgatctct
     2761 gctatgagct ggacccgtat gaattaaaac tggatgagat gatcgaggca gaaccggaac
     2821 ccgagatgat tgaagggctg cccgcctctg atgcgctgac gcctgccgat cgctatctcg
     2881 aactgttcga gcatgttcag tcggcgaaaa ttttccccga cagtaaaacc tttcccgact
     2941 gcgcacccaa aatggacccg ctggatattt taatccgcta ccgtaaagtg cgccgtcatc
     3001 gtgattttga cttgcgcaag tttgttgaaa atcacttctg gctgccggag gtctactcca
     3061 gcgagtatgt atcggacccg caaaattccc tgaaagagca tatcgaccag ctgtggccgg
     3121 tgctaacccg cgaaccacag gatcacattc cgtggtcttc tctactggcg ctgccgcagt
     3181 catatattgt cccgggcggc cgttttagcg aaacctacta ttgggactcc tatttcacca
     3241 tgctggggct ggcggaaagt ggtcgggaag atttgctgaa atgcatggcc gataacttcg
     3301 cctggatgat cgaaaactat ggtcacatcc ccaacggcaa ccgcacctat tatttgagcc
     3361 gatcgcaacc accggttttt gcgctgatgg tggagttgtt tgaagaagat ggtgtacgcg
     3421 gtgcgcgccg ctatctcgac caccttaaaa tggaatatgc cttctggatg gacggtgcag
     3481 aatcgttgat ccctaatcag gcctatcgcc atgttgtgcg gatgccggac ggatcgctgc
     3541 tcaaccgtta ttgggacgat cgcgacacgc cgcgtgacga atcctggctt gaggacgttg
     3601 aaaccgcgaa acattctggt cgcccgccca acgaggtgta ccgcgattta cgcgcgggag
     3661 cggcctcagg ttgggattac tcttcccgtt ggctgcgtga tactggtcgt ctggcgagca
     3721 ttcgtaccac ccagttcatc cccatcgatc tgaatgcctt cctgtttaaa ctggagagcg
     3781 ccatcgccaa catctcggcg ctgaaaggcg agaaagagac agaagcgctg ttccgccaga
     3841 aggccagtgc ccgtcgcgat gcggtaaacc gttacctctg ggatgatgaa aacggcatct
     3901 accgcgatta cgactggcga cgcgaacaac tggcgctgtt ttccgctgcc gccattgtgc
     3961 cgctctatgt cggcatggcg aaccatgaac aggccgatcg tctggcaaac gccgtacgca
     4021 gccggttact gacacctggc gggattctgg caagcgagta cgaaaccggt gaacagtggg
     4081 ataaacccaa tggctgggca ccgttacaat ggatggcaat tcagggattt aaaatgtatg
     4141 gcgatgacct tctgggtgat gaaatcgcgc gcagctggct gaaaacggtg aatcagttct
     4201 atctggaaca gcacaaaatg atcgagaaat accatattgc cgatggtgtt ccccgcgaag
     4261 gcggcggtgg cgagtatccg ttgcaggatg ggtttggctg gactaacggt gtggtacgcc
     4321 gtttaattgg tttgtacggc gaaccataat atttttacag ccagccgcta acttcctgct
     4381 ggctgtaaaa ttatcctctt caggaggaga tatttaacat cattgccgcc tgggtgcgat
     4441 ttttcacttc cagacggcga tacagggatt ccagatgcgc ttttaccgtt ccggtactga
     4501 tattcagcgc tctgccgatc tccttatttg attcgcccgc cgctaacatg gttaaaatct
     4561 cccgctggcg ggcgcttaac gatttgagat ctttaatgtc cttttccggc gtcgtccgcc
     4621 agtctccagg cagaaacatc atccccatcg ccgcactatt taccgccaac gcaaatgtct
     4681 cgacggttga atcacgaggc acaatggcca gcacattaaa atggataact tcctgtaacc
     4741 accgtttatt gcaatccgtc gccgtgatta acaccttaac ctcaggaaac tgcaccacgg
     4801 ttttttgcag caaccagtag caaaactcac catcctgatc gccatcgagc ataactaagg
     4861 cttcagggta actttccagc ttttgccata actcgtctgc ctgactggcc ccctgaatac
     4921 tcactcctgg aatacgctgc tgtaaactga ttttcattcc atgaataaat attgactgcc
     4981 tgtcaaacat gactatttgc ataactgaat ctccacctga atacgttaaa aagacttaag
     5041 tagtggaagg gtattacccg cgagaaaaaa taagaattcg ccatttggcg gtggccattc
     5101 tacagagatg acgcgtagaa aatagttacc gatataaata gttacagcta aacgcctgaa
     5161 attacatgtc gagggcacta tttaaaacaa ttttgaggat ttccttatat tggtggttag
     5221 tacgcatgca attaaaaatg aaactccgcg actacaagcc aaaataacaa atggcaatga
     5281 gacaaaaata agcacaaata gccaatacgt actctattca ctttaaaggg aatcactgaa
     5341 aaatacgctc tgtttaaggg gattcacctt tctcagaaag ctattccgcc cttttcctgc
     5401 tgagaaatcg ccacattcgt catgacaaca ttgtgaaacc cggcattaga tgttagaaaa
     5461 aacaataaac aatgcgatat gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga
     5521 aatggataaa attcacgcaa tgcagttgtt catcaaagtc gcggagctgg aaagtttttc
     5581 ccgcgcggcg gatttctttg ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact
     5641 ggaacatcaa cttggcaccc agcttctcca gcgcactacg cgacgggtca aactcacgcc
     5701 agaaggcatg acctattatc aacgggcaaa agatgtgttg agtaatctca gcgaactgga
     5761 cggtctgttt caacaggatg ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc
     5821 aggaatcgcg aaaaacctgt tactgccgcg tctgtcggaa tttctctatc agcatccggg
     5881 aattgagctg gaactgagta gccataaccg tccggtagat attcttcatg atggttttga
     5941 ttgcgtgata cgcactggcg cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa
     6001 actgaccatg gtcaactgtg ccagtccgca ctatctgacg cgctttggtt atccgcaaag
     6061 ccccgatgat ctgacttcac acgcaatagt gcgttataca ccgcatctgg gtgtacatcc
     6121 gttaggtttt gaggttgcca gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt
     6181 gacggtaaac agtagcgaaa actatctcgc cgccggtctt gccggtctgg ggattattca
     6241 gatcccgcgc attgccgtgc gcgaagccct gcgtgccggg cggcttattg aagtattacc
     6301 tggctaccgt gccgagccgc tctccctttc gctggtttat ccgcagcgtc gggagctttc
     6361 ccggcgtgta aacctgttta tgcagtggct ggctggcgta atgaaagagt acctggactg
     6421 accgactata ctttttaaga atgaccacga cagaaggaca aaagagcgga tgacgcagga
     6481 aaacgagatc aaacgtccca tccaggatct ggagcacgag ccgattaagc cgttggataa
     6541 tagcgagaaa ggcagcaaag ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa
     6601 agtccaacgc caaccggtta ttgcgcacct gattcgcgcg acagaacgct ttaacgatcg
     6661 gctgggtaac cagtttggtg cggctatcac ctatttctca tttttgtcga tgataccaat
     6721 tttgatggtg tcgtttgccg cagggggctt tgtgctggcc tcccatccga tgttgctaca
     6781 ggatatcttc gacaaaattc tgcaaaacat cagcgatccg acgctagccg ccacgttgaa
     6841 aaacaccatc aacaccgccg ttcagcagcg tacgactgta gggcttgtcg gcctggcggt
     6901 ggcactttat tccggcatca actggatggg taacctgcgt gaagcgattc gtgcccagtc
     6961 gcgcgatgtc tgggaacgct cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg
     7021 agattttatt tcgctgattg gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc
     7081 ggttgccggt tcggcgcagc aaatgattat tagcgccctg cacctgaaca gcattgagtg
     7141 gctgaaaccg acgtggcgat tgattggcct ggcgatttcc atcttcgcca actatctgct
     7201 tttcttctgg atcttctggc ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg
     7261 cggaacattt ctggctgcta ttggttttga agtgattaaa atcgtgatga cctacaccct
     7321 gccatcgttg atgaaatccc cctctggcgc agcatttggt tccgtgctgg ggctgatggc
     7381 atttttctac ttcttcgccc gtttgacgct gttttgcgcg gcgtggattg ccaccgccga
     7441 atataaagac gacccgcgaa tgccggggaa aacgcagcct taaaataggc cggatgcggc
     7501 gtaaacgcct tatccggcat acgattttat tcggcctggc tccccgtagg ccggataaga
     7561 tgcgccagca tcgcatccgg ctataatgcg cacataacct cttgaaactc gtccccagag
     7621 cctcttcagc catctatttg ggagcaaaca atttcattcc aactcataac cccagcatat
     7681 aaatccagtt ggtaactttt atttaacctg aaaccagttt tatccactat ttataaaatt
     7741 atgtgaagca tttcatagaa gaaaaatcac tggcctaaac attatcccct ttttgcctga
     7801 tttttgacca tttccgcgat ttgttacaca ttgaaatatc acttttgctg tgcgtaatat
     7861 ggctattcgt tagccaaaaa ataagaaaag attatgcaag caacagccac aacactcgac
     7921 cacgagcaag aatacacgcc gatcaactcg cgtaataaag tccttgtcgc ctctctcatt
     7981 ggcacagcca ttgagttctt cgacttttac atttacgcca ctgcggccgt tattgtgttt
     8041 ccgcatatct tcttcccgca gggcgatcct acggcagcaa cgctacagtc gctcgccacc
     8101 ttcgccatcg ccttcgtcgc gcgccccatt ggctctgccg tttttggtca ttttggcgat
     8161 cgcgttgggc gtaaagcgac gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg
     8221 gtgattggtc tgctgccggg ctatgccacg attggtattt tcgccccgct gctgctggcg
     8281 ctggctcgat ttggtcaggg tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg
     8341 gcgactgaaa acgccccacc gcgcaaacgt gcactgtatg gctcctttcc gcagctgggc
     8401 gcaccgattg gcttcttctt tgccaatggc actttcttgc tgctttcctg gctactgacc
     8461 gacgagcagt ttatgagctg gggctggcgt gtgccattta tcttctcggc ggtgctggtc
     8521 attatcggcc tgtatgttcg cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct
     8581 aaagcgaaaa aacaggtgaa gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta
     8641 accgtactgg gtacgttcat tatgctggca acctatacgc tgttttacat catgacggtc
     8701 tactctatga cctttagtac cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa
     8761 gtgttgtgga tgttgatgat ggcagttatt ggttttggcg tgatggtgcc agtcgctgga
     8821 ttactggctg atgcctttgg tcgccgtaaa agcatggtaa tcatcaccac gctgatcatc
     8881 ctgttcgcgc tgttcgcctt taacccactg ctcggttctg gcaacccgat tctggttttt
     8941 gccttcctgc tgctggggtt aagtctgatg ggtctgacct tcgggccaat gggtgcgctg
     9001 ttaccagagc tgtttccgac agaagtgcgt tacaccggag catcgttctc ttacaacgta
     9061 gcgtcgattc tcggggcttc cgttgcgcca tatatcgcag cctggttgca gactaactac
     9121 gggctaggtg cggtggggtt atatctggcg gcgatggctg gcttgacgtt aatcgccctg
     9181 ctgctgaccc atgagacgcg acatcagtcg ttgtaataag agtgaaaagc ctgatgcgct
     9241 acgcttatca ggcctgaatt tccatacaat atattgaatt cgcaaggatt tgtaggccgg
     9301 ataaggcgtt cacgccgcat ccggcatgaa caaagcacac tttgccaaca atctaaatct
     9361 gtttaattgc cggatgtcag acatccggca attatcatca cttcttcatc tgcgacaaaa
     9421 tcgtccgaca ctgattagcc tccccttcgg aaggtgagat cagtgccagt aacgccgccg
     9481 ccggtgttac cagcgttgcc agtgccgcag caacagcacc acgggcaatc agcggtccgg
     9541 ctttcacccc agcctgcgga tttttaaacg tcccccgcac atacagcggc gaacgcagtg
     9601 tgataatccg aattccttta ctctccggat caatagtcaa atccagctgt tccgaagcaa
     9661 aacttgccgt gccggtaaca ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg
     9721 ggcgcgccac gccgttggca atattcagat tcgccgccgc gcagttcacc cgcacctcat
     9781 catcaccaaa tatcgcaccg acaatgtagt tgccgacatt cagcccaaca atctccatca
     9841 ggttgcggct caccagcccg tcattcatca acagtttcag gttgccgtta ctgttgccta
     9901 aaagtgccgc caccgagtta ccgctaccgc gtagttccgc gtcaccgttc atttccccca
     9961 gcgtcttctg catcagttcc acatcgggca tcagttcttt cagtttcagt cgacgagcct
    10021 gaatatctgc ccgcccctgc atcggctttt tatcgccttc cagatgaata ttcgccgcaa
    10081 tgctgccgcc cgccatgcca aatttcagcg gttgcaggcg caggtcagca tttttgagga
    10141 tgatatgagt agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt
    10201 tgaagcgaac atcggcatcc ataacgtccc atttatcggt ttcgaagcgg tcataaggca
    10261 gcactttgcc cgcaggctga acgctttttt cgcccttctt ctgttcagac cgtttcgact
    10321 tttctgcccc tttcccggaa tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt
    10381 gccgcgactc gacatcacct tccagttttg gtcgtggctt tccggtggtg tagaccagag
    10441 aaccgtggat atcgctatca ccaattcgcc cattaaaacc gcgataatca aagaccgacg
    10501 atttttcagt gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg
    10561 gcagcagaac gcccgtcagt tcatagagat cacccagtga atcgccagaa aatttaagcc
    10621 gtaaatcgac accgcccatc ttcattgggt cattcacgac gccatcaaaa gcaacacggg
    10681 tgttaccaga gcggaaatca gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca
    10741 gcataccgcc tatttttccc gtacccgtga gcggttcacc gttatatcgt ccctgcgcct
    10801 tcaggccaaa aacgtaatcg cccacctttt ctttatccgc tttacctttc gatccagtaa
    10861 cttcgctgaa cggcagcggc ttgcctaagg gatcaacaaa aatctccaga tccgctttgc
    10921 ttactttgtc atcaatggcg atccgccctt gatcgaaaag aatattatcc agccgaaacg
    10981 accatgccga cggctttgca ttcgcgtctt tgttatcatc gttggcaaga ttaaacgtcc
    11041 agttattgtt cttttcagag aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc
    11101 acggcagcca gaccgttttg gtcagcagcg ccagcggggc cagcgttgcc tctacgcgtg
    11161 gcaaatgcac catcgtgact tcgggaatat ccggtggatt gccaagaatg atgtcttccg
    11221 cgtgtacatg gggccacggc acccagctgc gccagccagt ttcttgtttt tgccgctccc
    11281 acaccacgcc cagatcgcca cggatagcga acggacgatt caactccgca gagactttct
    11341 ggttgatagt cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga
    11401 caatcaacaa caagaaagcc cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg
    11461 ttcgcttcct catattcttc ctgtgccagt cctaaagata gtccagccag gcggaaaatg
    11521 aggcagctta tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa
    11581 gaaaccagcc ttgtgcggcg aatgcgggcg agttctgaac atcacgccac tcttccggcg
    11641 tttctacgcc ctcgacaatc accccgcgac aatagcgatt cattagatgt aaaagctgag
    11701 aaaagagtgt gcgtccttcc ggcgactgac gcagcatcac aaacagttct cgcgcgattt
    11761 tgatgtagtc ataacgcact tcacttagcg cagagaaatt tgccatcccg gtaccaaaat
    11821 catccagcca cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac
    11881 ggatatgctc caccagttcg aaacacagcc agggaagacg ctcaatctgg cgcaggattt
    11941 ttggttgctg acgcagggcg atgagcgtag ggccatcaat attgaccgat gccagcaggc
    12001 cgtgctctat aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct
    12061 ccatccgatg gctgacggtg atttcagtaa aatagcgatc cggcggcagg cgttgcgaag
    12121 ggttcaaggg atgcgtgacc accgttaata gctccacggc cattaaccgc ccgcatgttt
    12181 gatagatcgg ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca
    12241 agctctcgat gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag
    12301 atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa agattatcgg cgcgtaaggg
    12361 cagaacttta ccgcctggcc ggggtaaatt tcctcactcc acaacggaat ctttcaggaa
    12421 tacgttctgg ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac
    12481 cacagcgcaa actaacgcta attttttaca gatcaggttc acgactatgt ccaaaaagat
    12541 tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg
    12601 tttcggcggc gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc
    12661 attaaccgtt cattacgtaa cggcgctggg aacggacagt tttagccagc agatgctgga
    12721 cgcctggcac ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc
    12781 gggcctttac tacattgaaa ccgacagcac cggcgagcgt acgttctact actggcggaa
    12841 cgaagccgcc gccaaattct ggctggagag tgagcagtct gcggcgattt gcgaagagct
    12901 ggcgaatttc gattatctct acctgagcgg gattagcctg gcgatcttaa gcccgaccag
    12961 ccgcgaaaag ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa aagtgatttt
    13021 cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca
    13081 acaaatgctg gaatgcacgg atatcgcctt cctgacgctg gacgacgaag acgcgctgtg
    13141 gggtcaacag ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt
    13201 ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga
    13261 tgttccggcg gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc
    13321 tttcagtgcc ggttatctgg cggtacgtct gacaggcggc agcgcggaag acgcggcgaa
    13381 acgtgggcac ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga
    13441 ggcgatgcca gcgtaaacgc agcatatgca aaaggctgga taaagtgtta cccctctatc
    13501 cagctcatcc ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtgc
    13561 aggtggaata tccgtcactt caggatgtac gtcatccgtg gcgacagagg tggtcgcagc
    13621 ggcggtagat ggggccatga tttgatccca gaccgcctgc aacgctttca tgttaaattc
    13681 cggctcgcct ttcggctgca gcagtattaa cgccatatca ttcgacaact gttgacgcag
    13741 atcctgattt aacatctcta ccgtcaggct attaaggaaa tcctgccgca gtttctgata
    13801 ttgctccggc gcgatatcga caacctgatt ttgcaacgaa cgcatccgct gacccatcag
    13861 aatatcggta tcagctcgtg cataggcggc aaacagtttc tgcagctcca gtttcttttg
    13921 cgccactaac gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc
    13981 acgcgccacc agattaaggt tgctgttcag cttgtcgttt ggtgattcga tgttgatggc
    14041 acactgcgca cgcagataca gcacacggca gtcaaatcca agaccgatgt ctttgctgtt
    14101 actggcactt aacgcttgct gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg
    14161 ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat
    14221 agataaccgg tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg
    14281 agaaagcgtc ggcaccggag ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt
    14341 gtttatttgg tcgacaaccg agcgcgcatc cacgtttccc accaccagta gcgtcattgc
    14401 atccggggtg taccatttct gatagaaatc tttaattttt tccgcttcaa cgggttgttt
    14461 cagcggatcg gcaggatcgt gacctaacaa ggttgaccct ttcagacgat agcgccacca
    14521 gccctcttta gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgtggtt
    14581 gatggtttct ggtgtgatgg tcaatttgcc agtggcattt gccagataag agagcgcttc
    14641 tttcagcaaa tcgttacggt tattgggcaa actcagatta aacagcgtgg tgtcataaga
    14701 gacaattacc ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc
    14761 ctgtgctgcg tcaaggccac cgctttgcgt tagcgcaata cgagggatgg cgtgactgta
    14821 accgctctgt tgtgtacttt cggcgagcga accggtatta accagcaggc gaatttcaac
    14881 acgatcgctg ggacgctggg gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt
    14941 cccctgttgc catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc
    15001 catcatcagc aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga
    15061 tcaactatcc tggtaataaa aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa
    15121 cacttcgtgt tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa
    15181 ctcacccagg tggtaagttc aggcacaggg gtcaattatg cgcaaacacc cgcactcggg
    15241 gaagggagtg cgggcataag tgatgagatt aagaggataa ttcgtgcgtt ttgccatccg
    15301 gcgcacgatt attcagcaca tcgtccagtt ttttgtggtc cagttctttc acccacttag
    15361 caacgacaat ggtcgctacg ccgttaccga ccaggttagt cagcgcacga gcttctgaca
    15421 taaagcggtc gataccgagg atcagcgcca gacccgctac cggcaaatgg cccaccgcag
    15481 agagcgtcgc cgccagcacg ataaagccac tacccgttac ccctgccgcc cctttagaag
    15541 aaagcagcaa cacgattaac agcgtgattt ggtggacgat atccatctga ctgttagtgg
    15601 cctgggcgat aaacaccgcc gccattgtca ggtatatcga tgtgccatca aggttaaacg
    15661 agtagcctgt cgggatgacc agccccacca ccgatttacg gcagccgagt ttctccatct
    15721 tgtcgagcat acgcggcagc gccgactcgg aagatgaagt ccccagtaca atcagcagtt
    15781 cttcacggat gtagcggata aatttgaaga tactgaaacc agtcgcttta gcgattgaac
    15841 ccaataccag caccacaaac aggatacagg taatgtagaa acagataatc agctgcccca
    15901 gttgcaccag tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac
    15961 caataggtgc cagacgcatg atcatattga tgatgccgaa gatgacctgc gagaaacttt
    16021 cgatgacgtt aaaaatcagt tggcctttgc tgcccagacg gtggagcgca aaaccaaaca
    16081 gtacggcaaa cagcagcacc tgcagaatgt taccgctggc aaatgcgcca atgacgctcg
    16141 ccgggatgac atccataatg aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa
    16201 ccgctaccgc tttcgcatca agcgttgccg gatcgacgtt cattccggca ccaggctgca
    16261 cgacgttaac gatgataaga ccaataatca gcgcgatggt actgacaatt tcaaagtaaa
    16321 gcagtgcgac tgcgccggta cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg
    16381 ttacgacggt acaaaagatg acaggagcga tgatcatctt aatgagctta acgaagccgt
    16441 cgccaagcgg tttcatttgc tcgcctattt caggatagaa atggccaagg agaataccaa
    16501 tggctatcgc tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt
    16561 gtcctttagt aaaaccacag gtcttgtaag gttatggggt acctgcggcc ttaaaataac
    16621 acccagacaa catcacagaa atgtacctgg atcataattg aaacaaaaag gttaaaaagt
    16681 ttgagctggc tcgcacaaac cagcactttt taaagttttg taatcagttt ggggtagcta
    16741 cttttcttcc aggtaactct cttcgaagat ttcaataggg agtgggcgag caaaaaggaa
    16801 gccctgggca ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt cagtctccac
    16861 gccttcggca atcatttgta agttcaggct ctgcgccagc atgataattg cagcaatcat
    16921 gctgctatct cccggcaagc cttcaacaaa cattttgtcg attttcagta cgtcgattgg
    16981 caacgatttc atatgctgca gctgacgcag ccctgcgtag cccatgccga aatcatccag
    17041 cgccacccga actccggcat tgcgcagcgg acggaggatt gccaccgcag catgagggtc
    17101 gtcaatacgt cggctttctg tcacttccag aatcagtgtt cccggctgaa tgcgatagcg
    17161 ggttaacagt tccagcatat ccgccaccat attcgggtgc atcagttgca gcgcagagag
    17221 gtttaccgac aagggcagca taatgccgcg ctcttgccag gctgcaagca atcgacagga
    17281 ctcttccagc acccagtgac cgacggtaac catcagccca cagcactcaa tgcgatcgat
    17341 taagccatcc ggcaggtccc aactgccatc cggttgctgg atacgcagta acacttccgc
    17401 actgaccagt ttaccgctgg tcatctcgac ctgtggctgt aaccaaatag caaactgatg
    17461 attttccagt gcattaagga tatcgctctc ttccgtcaac cgcttctggg cggcttccat
    17521 ctgctgcgga tcaaagaact gaatctgatt cttgccttta tggcgagcgg taaatgccgc
    17581 agaaatagcg cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca tcgccacgcc
    17641 aatgctacag tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt
    17701 gagcacttgc tgacctaagg tgattgcgtg ccacggttcc tgtacaccgt tggcaatgac
    17761 agcaaagtca taaccgctaa tctgcgcgag gatcatacgt ggcgacagta ccgatttgag
    17821 tttttccacc agcgtcagca gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt
    17881 atcacgcagg gtttcacagg tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac
    17941 ctgctccagc atctccatca gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat
    18001 cgcattttcg ttctgttctt cataatggcg ctgcagcaat tgctggttga ggttgtaact
    18061 gcgcaccaac ataccgattt catcgtcctg atgcagacgc ggtaatgcca gttggtgacc
    18121 aacaagctcc ttggctggga tggcgttaag ttcgcgagca atattgcgta acggatgcaa
    18181 aatcaggcgg ttaatgcacc agctgatggc gacggtcagg ataagcgaca aaagtaagta
    18241 aatggtcact aacgttgaga gggtgctcat cacgaactta tacatacgga aggaatccgc
    18301 ctgtagtacc agataggcaa ttggctgcgg gtttgccgga cgttcgagcg agtaaacgcc
    18361 cagcgagatt tgaaccggta gctcaaacag gcgagtaacc attaccggca ccgggcgctc
    18421 tggaataaaa cttttacgca gcgcctggaa ctggttaggc agcactacat aggcacggct
    18481 gaccacgcct gccggtttaa tgctggcaag aatagcttcc gcttcgggaa tatcgccttt
    18541 caaaatagct gaagataagg gttcgcggac agagcgagca atgctttcca gttgcgtagc
    18601 cgtgttatag cgattctgct ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac
    18661 gaacaccagg acaacggctg ccaccattgc catctgcttg attgttaacg agcgacttac
    18721 gcgcaaattg tctccccccg gaaatcccag attgccgccc gagtataccc gatcgcagcg
    18781 gcattaagag aggcgctatc tgaaaactta ccagtcggcg taaggtatca gcggctgcgg
    18841 cggtaaatcc atgtcaccct gccatccggc ggcggaataa cgtacgtaga gcaggaaatg
    18901 gctgggtgcg taatctttcg cctgctggat atcaattgcc gtgccaacaa accagttgga
    18961 agtaacacgt cgttcaagta atgcccgcgc cgtgtagccg aagccctgac tgctgccgcc
    19021 atcgttggat tgccgcgcag cttcttcctg ccagtcggtc gggatcagat tcatcagcgg
    19081 ataacgcggc atggttttgg tgcgtgaatg cgaccacgag ccagacgcac ccagctccca
    19141 cgaccagttt tccgtgcgct cccgccacat caccggtatg gcaaacgaca ggtattcctg
    19201 cggactgtag taaccgccct gaccgagtga gtagccactc agatctttgt cgtaatgcca
    19261 gatcatgttg ttcaggccga ttgtgacgcg gcgattgttc tggttaatga ccttatagta
    19321 atagcccgtc atccagcgca cgcgccagtt atcttcgaca tttttaccgg ttaactggtc
    19381 gccactaagc gatgcccaga cgccgtttgc ttcaccttta tcgtagctca gacttagccc
    19441 cacaccgtcg gcacgtacgc caccccattt tttcccggta ttgctcgggg agtctttttg
    19501 cccaccaaag gccagcaaag aactggagat gggccgacgg tgggcgttaa cggtgtaacc
    19561 cagcggcccg atatcatcgc tgtaactgat gccgccgacc acatccacca cgttgaagcc
    19621 catcggcgtg gtaccgatat cccagctcca gacgtcattt cgccagccga ccgccacgct
    19681 ggcaccggag tccgactggc tgcggttgcc gctacagtcc tgtaatgtac aggtgcccca
    19741 gttgtcatcc catttgccat cggcattagt ggagaaactg ccgacgttca tattgacgaa
    19801 atcactgcga aagaacatcc gcccgtcaga atacggcgca tccacctgca acatggtagt
    19861 gtgcgctttc agatcggagt aaccaccggt gccgctcgaa ccccagtaat cgtgctcaag
    19921 ggtgacgtta agatcctgct ggcgatagag gtccgccgca tcgctgcgca cgccacgttt
    19981 cagccagtca tctttctcgt cgttacgggt cagtcgggta aaggtgtcgt tatcctgcgg
    20041 acgcgtcgta gtcacaccgg atgcgaccat ggcgtctttg taggtttcca gcgcctgcgt
    20101 tggatcgccc gcctgcgctt caaacttcgc accatcacgc agcaccatcg cgctttccat
    20161 cgacggtggc tgagattttg cccgcgggat caacttatta aacgtccgct gcgctgctgc
    20221 ggtatcgcca agctgcgcct gtgccagcgc cacgcgccgc tgtgtgttca gcgaggcgtt
    20281 atcggtagcg ggcagtttcg ccagctggct acgtgccgcc gctttgtcac cggcagcaat
    20341 atccacttcc gtcagaccaa gaatggcgtc ggcgttagct ggctcccgcg tcaggacatt
    20401 ctgatatgca gcgcgggcgg cggtgtaatc acgtcgttgt tgcgcccagt cagccagcgt
    20461 gaggtcaata cgcgtggaag gtggttgctg gcgcagcatc gcttccgctt ctgcctcttt
    20521 gccgctttct cgcaggcggt tagcggtttc cagcacctga tcgctttgca gtcgattaac
    20581 cagctcctga atattgctgt tccactgcgc acgcggcagg ctgttgatat gcgccagcgc
    20641 cgctctgtcc tggtcatgac cagagagata cagcccgtaa gcgtaaacct gctccgggtc
    20701 attcggcttc tgctgcgcca gattgcgcat taacgtatcg gcctggctgc gttgtccggc
    20761 ctgccagaga tcctgcgaaa gtcggtaagt aatccataca ctgccggggt ccagcgccag
    20821 tcgttgccgc tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc
    20881 ctgctgtgcc agacggtcgt tttgcaggct gcgttcgata tcatcaatgc tacgccgctg
    20941 actggcagag agcgaggcga taaacgcttc agctttttct ggcgattgct ggcggtaaat
    21001 atttgccagc ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg tctgctgata
    21061 ataacgttcg gcggcgggat aatcttttcg cgccatcgcc acatcgccca gccccagcac
    21121 tgcataactg tcggtgttat cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc
    21181 aggattattg gctttcagcg cagcatcgcc ctgctggatc gccagccagt agcggttcac
    21241 tttcagcaga ctattccatt tgtcgttgtt gctgctgtgc gggtccagtg cgagggcttt
    21301 ttccagattc gccactgcat tggcgcgatc gcctttctgt gaatatgcct ggcccagcgc
    21361 ccccagagcc tcactgtctt tcgggttcgc ccgcactgcc tgttgtagtt cgggaatggc
    21421 tttacccgcc ataccagagt ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg
    21481 atcggccagc tgtttttgct gttctgccag ttgcgattgc gcagccgcca cgctatcgcc
    21541 atcactaaag atcgaaagat attttttcag cgccgacaca ctggcatcgc tgacgggcat
    21601 gtctttaatc tgcccgtacc agattttaga ggccccttcg cgcccggcgt tcgatttcgc
    21661 catctgttcc aggacggcaa aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc
    21721 cagattgttt tgcaggcccg tattgcccgg cgtatccgca ttaatgcgtt ttagctgatt
    21781 aatcgcttcg ccacggcgag ccggaatttt cgccaccgta ctccagtact cgacagcaat
    21841 gtcaccttcc ggcggcgcac cgttgaacag tttgttgtaa ctcgccacag cttcttccgc
    21901 atgaccggta gtcgcctgca atcttgcctg ttgcagtgcc tgacgaccat ccggcgtgga
    21961 aagtagcatc gtagtccgcg acgatttata cgcatttgaa ctcggcgcta actgcgacag
    22021 ccgatcgagc tgtttttgcg cgccatcaat atcgccctga cgtaacaaag aacggaaacg
    22081 ggcggcaacg acgtccgggt tattcggatc aataagttcc agccgatata acgactgttg
    22141 caccagatct tcacgatggg tcgcttcgcc taaccgaact tgctccagca actgttgctg
    22201 agcggttggt gctgcctcga ccatcggcat gacggccaga ccgagggaaa gcgtgaatat
    22261 gtttagtgtg aatttgcgca ttcctggccc cagtcaggta ataactcacc ttttgtcgag
    22321 aagcggaaac ggtgttgatc ccagccttgt ccaaacaggg tcagcacata gttgtaatag
    22381 gcatcgctgc cgggaaagtt atcggccacg cgctggcgct gaacggcctg cgcatcgcgg
    22441 ttttgtaaaa aaggcagcat ggcggcagaa aaaccgaccg gtcctttacc ctgcgctttc
    22501 cccgtagcca catccacttt ttccggcgga taaccgtttt tctcagtgaa tgtcgccatc
    22561 ggtttaaacc ggttgagcat ccgcgctttt tgcggatcgc tgtcaggcat catgcctacc
    22621 cacatgtaaa cgcggatagc gtcgtagctg ctgatcaatg ttttttcggc ttttagctgc
    22681 cagcctttgt ctttctcata gcgcacccag tctggcgaaa agcctttcgg ggcggtttcc
    22741 agcaataaac gttgattggt ttcgcgcagc gtagtccacg gcgcgccaaa gcgggtgaaa
    22801 tactgcgcca gcgtcggcgg caggtagctg gggttaaaac gccagctgtt atcctcagca
    22861 aaacccactt tgcccggtaa caacatggaa cccagcccag gcaccgtcac cacttcctcc
    22921 cgcgcgatac gttttagcaa cgcgctgccg atgtcggtat aacgctgctc tttccacaaa
    22981 cgccccgcct ccagcaatga ccaggccatc cagacatcac catcggaggc cgaattgctg
    23041 tccagcactt cccacttact gttctctttc ttgccccaca gccaggcggg caaaccttct
    23101 tttaaagaac cctgagcgag attgttctgc gtccagtcga gaatattatc gaaagctgca
    23161 cggtcgttag ccgccagggc aaagaacata ccgtaacttt gcccttcgga ggtggtgatt
    23221 ttgcgcgcgt cgctggggtc gatgacgcgc ccttcctgac tgatgtaatc ctttttaaac
    23281 tgctcccagg caggccaggt acaggctgcc tgaacactaa aggcagccag cagcagcatc
    23341 gtcacgagtc cactacgcaa cacattcatc ttcaattact cgttatccgg gttaagacga
    23401 cgacgactaa taattcgcag cagacgccac agtacccatg ccagcaatat cacactgatt
    23461 gccgccagca ccgccagcag aatcggatgg tttgccagcg cataccacaa gcgctcgaac
    23521 cacggcagat gacctacgta ataaacgtcg ccaacacgta ggctgttgat accggactcg
    23581 cggatcaccg cgaccgaacc gaacatggtg gcgcgtttgc cgctatcgtt caccgcatcg
    23641 ttaagcattt cataaccgcg tgggctatct gccagcagcg caatcacgct gcgctggtcg
    23701 ttatacggcg actggaagcc aatcaccgcc gccatcgcac cggaagaggt cagcgttgac
    23761 cgggtttctg ccgcgcgatc gctctcgtcc ggtacaatgc cggggaacgg ggtctggcgc
    23821 atcggtgttt tcacccagct ttcggtcgcc tgcaccaata ggtcgatctg cttatcgtct
    23881 ttcagtttgt ccgggatacc accgatgatc atgatgtcgg catctttgcc ctgaatggtg
    23941 ctgccatcat cggtcaccgt cagattaatc gccgggaagc ccgtctgtgc gccgataaaa
    24001 ccaacagtat tcagcaacgt ttccatctgt gcttcgttag gcgctttcgg catcacggtg
    24061 atggtttgcg acagatccgc catccggctg aatgggaagc ccgcgttagc aaaggcgcgt
    24121 agatccggca tcgggatgaa gtggtaatac ttcgagaagt cgatggtgga atcgtcacca
    24181 atcaccacat gattctgcac cggctggaag gtaatacagt tatctaccga accgcctggc
    24241 atcgggttca tatactcaaa gtcgaagcgc agctggttgg tcgcgcccag tttcagcgcc
    24301 ggaatagaga catctgtttt gccatccagc aaaccttgta ataccggaat ccgcagcagc
    24361 aggcggttcg cctcctgttt gctgctcagg ttgaaggatt gcaggaactg gttattcagg
    24421 ctgatatcca tccgcgaact gtctttcacc ggcggcatgg tgtagcgata gttgatatcc
    24481 atatcaatgc cggtactgcg catcaggtag agatccggtg gcaggtttag cgaaacgtta
    24541 atcgctgctg gctcaagacc gctggattgt aactgttctt cataggtttt cagttcacca
    24601 aaggtgaccg gacgatcggt acgtacccag ttcggcgcat cgtaaggctt acgcggtagc
    24661 agcggtttca cttcattcac taccacgctt tcaccacgga acagaatgtt accctgagcg
    24721 atacctttcg ctgcctgcaa caggtctttg tcgtcacgac caaacaccac cagcagtttg
    24781 acgtaaggat tctgcggatg gttaatcatc tcaatcaccg gggcttttac cgccggatga
    24841 tcgcgcagga agtccggccg tttgtcgttg gttgcaaaga caatggcatt acgatccggc
    24901 agttggttat agagcaccgg gaagttctgc ccacgccagc cagaacgcga accaaaccac
    24961 gaggcgacaa tggcagaggc ttgttgcagc ccaacatccg gcgcacccgc aaagaccatc
    25021 ggcaaggtgt tagtacggtt atcgcgcggg tcaaagaatg gcaccgggaa gtgtgacagg
    25081 tcattcttca cattcagggt ctgataggtc agatccagtc cactgctccg cccaacatcc
    25141 agccaaagcg tggtgctggc cgggttttcg cacacgtcct gataatggcc gacaaactcc
    25201 agccgtacac ggttgaagtc ggtaataaac agtgggttaa tgggcatttg cgccagcgtt
    25261 tttttaccca actgttcttt ggtcactggc agcacgccca tcagttcatc attgagataa
    25321 acctttaact gcgactggac aggcagtaac gatggcgatg gggtgtattc gaggttgagc
    25381 atcgccttcg tcaccacttc atcgctgcgc ataccaaact caatgctgcc gttcgggtta
    25441 atgccacgta gcaccatgct gcccggcggc ggtgcaattt gcgcaaaggt cagcttcaca
    25501 tcacgcgaag gaccgttctg cgccacgact ggcgcatcag cgccctgcac gcccggcatc
    25561 acttgcccca cctgcggatt ttgttccgtc tgggcggcta cagctggctc agcattgatc
    25621 agtggttgcg ttgctggcgt cgcctgcgtc atgaaagagg ggaacgcact catccccata
    25681 gccactgcac aaatccagaa tagttttctt ttcatcgcgt tatcatcatt gttgagccaa
    25741 agcctgatcc gatggttgtg ccgtttcgct ccgctccggg cggcgcggaa taaacgatac
    25801 aacccaggaa accagagaag tcagcacacg gaatatgccc ttcaccgaag aaggcgcaaa
    25861 ctccgccaga tggcggtagc cacggaagcc gagcttcaga atatccagca gactttccag
    25921 cggcttatct tccggatagc tgtcctgcca gagcgcccat gtatccgcac gggcaaacgt
    25981 acactgcaca aaatcgatat gttgctgggt ggtgagcggc attaatttca gcccaacttc
    26041 attacccatc acgcgcgcca cctgggtcgg gaagacgtat tcctgctgac cgcgtttaag
    26101 caacagattc actttctgcc cttccagaat ctgcgcctga ccgttgatct tgatccccaa
    26161 accaccgtcg gagaaatcct gaacggtaca cgagaagagg tgaccatctt cgcgggcaat
    26221 tgccgcgggc atcgtcatct ccacgcggtg cgatcggcgt acctgtttgc tttctaccga
    26281 taccgcaact gcgccgccaa gaacaatcag gttgtagaat acccacacca tactgacgac
    26341 cacggtgagc atctcggttg gcgggccata gaagtagcgc cagatgccta ccgcaacgcc
    26401 caccaggttg agcaggacaa ggaagatgta gggccgcgag atcacccagt cgacg
//
LOCUS       DACAPB010000049        26243 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.049, whole genome shotgun sequence.
ACCESSION   DACAPB010000049 DACAPB010000000
VERSION     DACAPB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.049"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..251
                     /locus_tag="IT904_001823"
     CDS             <1..251
                     /locus_tag="IT904_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3191616.1"
                     /translation="EQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEA
                     ISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            405..2402
                     /locus_tag="IT904_001824"
     CDS             405..2402
                     /locus_tag="IT904_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAP3191617.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2465..3742
                     /locus_tag="IT904_001825"
     CDS             2465..3742
                     /locus_tag="IT904_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAP3191618.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSISTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPGIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            3990..4646
                     /locus_tag="IT904_001826"
     CDS             3990..4646
                     /locus_tag="IT904_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP3191619.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYFKSKEDYAQTVLNAYDSFFEHKLKKHLHESSCTPMVRLENFI
                     NDACEGIKKYNFTRGCLVGNMMQESPGLPQSFIKVLQNILESWQALVAACLSDALSSG
                     EISSNMNNTQLAAIFWSGWEGAVMRSKLYCSTEPVYDFWSYFKTSVRYQSSQEATTGQ
                     "
     gene            complement(4827..4934)
                     /locus_tag="IT904_001827"
     CDS             complement(4827..4934)
                     /locus_tag="IT904_001827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP3191620.1"
                     /translation="MGNMAPFILRGVTLKGIDSVMFAKEKRAAIWQQAV"
     gene            6054..6152
                     /locus_tag="IT904_001828"
     CDS             6054..6152
                     /locus_tag="IT904_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAP3191621.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
     gene            complement(6154..6936)
                     /locus_tag="IT904_001829"
     CDS             complement(6154..6936)
                     /locus_tag="IT904_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP3191622.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            7242..8162
                     /gene="rbsK"
                     /locus_tag="IT904_001830"
     CDS             7242..8162
                     /gene="rbsK"
                     /locus_tag="IT904_001830"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP3191623.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKINGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            8190..9506
                     /gene="fucP"
                     /locus_tag="IT904_001831"
     CDS             8190..9506
                     /gene="fucP"
                     /locus_tag="IT904_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAP3191624.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            9518..10531
                     /locus_tag="IT904_001832"
     CDS             9518..10531
                     /locus_tag="IT904_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HAP3191625.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRCEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            11016..11207
                     /locus_tag="IT904_001833"
     CDS             11016..11207
                     /locus_tag="IT904_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3191626.1"
                     /translation="MVTLALQPRGSVARIAREHDINDNLLFKWLRLSLIKGRISRRLP
                     LTNSSGIGVELLPVEMTLG"
     gene            complement(11454..12710)
                     /locus_tag="IT904_001834"
     CDS             complement(11454..12710)
                     /locus_tag="IT904_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP3191627.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(12723..13010)
                     /locus_tag="IT904_001835"
     CDS             complement(12723..13010)
                     /locus_tag="IT904_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP3191628.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TSSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVVLNELGAL"
     gene            complement(13026..13469)
                     /locus_tag="IT904_001836"
     CDS             complement(13026..13469)
                     /locus_tag="IT904_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP3191629.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY"
     gene            13740..14771
                     /locus_tag="IT904_001837"
     CDS             13740..14771
                     /locus_tag="IT904_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP3191630.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NSDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPE
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            14899..15423
                     /locus_tag="IT904_001838"
                     /pseudo
     CDS             14899..15423
                     /locus_tag="IT904_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(15461..15649)
                     /locus_tag="IT904_001839"
                     /pseudo
     CDS             complement(15461..15649)
                     /locus_tag="IT904_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            15710..16843
                     /locus_tag="IT904_001840"
     CDS             15710..16843
                     /locus_tag="IT904_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP3191631.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKFQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLIAITLVAELGDFSRFSNPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRTLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            complement(17096..18445)
                     /locus_tag="IT904_001841"
                     /pseudo
     CDS             complement(17096..18445)
                     /locus_tag="IT904_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
     gene            complement(18494..18841)
                     /gene="tnpB"
                     /locus_tag="IT904_001842"
     CDS             complement(18494..18841)
                     /gene="tnpB"
                     /locus_tag="IT904_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP3191632.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18838..19242)
                     /locus_tag="IT904_001843"
     CDS             complement(18838..19242)
                     /locus_tag="IT904_001843"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP3191633.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            complement(19288..19767)
                     /locus_tag="IT904_001844"
     CDS             complement(19288..19767)
                     /locus_tag="IT904_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP3191634.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRTGIKVRFTTAADLLLQLSTAVSVVSAPSGRS"
     gene            complement(19779..19958)
                     /locus_tag="IT904_001845"
                     /pseudo
     CDS             complement(19779..19958)
                     /locus_tag="IT904_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(19959..20324)
                     /locus_tag="IT904_001846"
                     /pseudo
     CDS             complement(19959..20324)
                     /locus_tag="IT904_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(20488..20572)
                     /locus_tag="IT904_001847"
     tRNA            complement(20488..20572)
                     /locus_tag="IT904_001847"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20536..20538),aa:Leu,seq:caa)
     gene            20768..21787
                     /gene="ahr"
                     /locus_tag="IT904_001848"
     CDS             20768..21787
                     /gene="ahr"
                     /locus_tag="IT904_001848"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAP3191635.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            21917..23419
                     /locus_tag="IT904_001849"
     CDS             21917..23419
                     /locus_tag="IT904_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAP3191636.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLDIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRPTPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(23580..24662)
                     /gene="lptG"
                     /locus_tag="IT904_001850"
     CDS             complement(23580..24662)
                     /gene="lptG"
                     /locus_tag="IT904_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP3191637.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(24662..25762)
                     /gene="lptF"
                     /locus_tag="IT904_001851"
     CDS             complement(24662..25762)
                     /gene="lptF"
                     /locus_tag="IT904_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP3191638.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            26029..>26243
                     /locus_tag="IT904_001852"
     CDS             26029..>26243
                     /locus_tag="IT904_001852"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767120.1"
                     /note="catalyzes the removal of N-terminal amino acids
                     preferably from various peptides; a cyteinylglycinase,
                     transcription regulator and site-specific recombination
                     factor; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAP3191639.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSER"
BASE COUNT         6651 a         6285 c         6508 g         6799 t
ORIGIN      
        1 aggagcagtt tggtctgaaa acatcaatgt cgcgtaaagg taactgttac gacaacgctc
       61 cgatggaaag cttctgggga acgctgaaaa atgagagcct gagccactat cgttttaata
      121 accgggatga agccatctca gtaatacggg aatacattga gattttctac aatcgtcagc
      181 gtcgtcactc tcgtctgggg aatatctccc cggcagcctt cagggaaaaa tatcatcaga
      241 tggctgctta aaaaaagaac aaatggtagt gtccgctatt gccagtacac ctcaacattc
      301 caccatgcat tccgattaac gccgcatagc cagttgaact ttgctacttt gtgagaggta
      361 gtaccttcta tccagtgcga atttaattaa tggaataaat gattatgagt gaaaatgata
      421 caatcccaaa gaagtctaca agtcagatta acaaagcggt attctttaca tctgctttgc
      481 taattttcct tcttgtcgcc tttgccgccg tattcccgga tgtcgccgac aaaaatttta
      541 aactacttca gcaacaaatc ttcacgaatg ccagctggtt ctacatcctt gctgtggccc
      601 tgattttact gagtgtcacg ttccttggac tctcacgcta cggtgatatc aagctgggcc
      661 cggaccatgc gcagcctgat ttcagctacc actcctggtt tgcgatgctt ttttcggcag
      721 ggatggggat cggcctgatg ttctttggcg ttgccgaacc tgtaatgcat tatctttcgc
      781 cacccgttgg cactccagaa accgttgcgg cagctaagga agcaatgcgt ctgacctttt
      841 tccactgggg actgcacgca tgggcaattt atgccattgt ggcgctgatt ctggcgttct
      901 tcagttaccg tcacggtctg cctttaactc tgcgctccgc actctatccc attattggcg
      961 atcgcatata cggacctgta ggacatgcgg ttgatatttt cgctgttata ggcacggtct
     1021 ttggcgttgc gacatcactg ggttacggtg ttttgcaggt gaatgccggt ttgaaccatc
     1081 ttttcggggt gcccatcaat gaaacggtgc aggtaattct gatcgtggtc atcacggggt
     1141 tagcgacgat ttcagtggtg tccggtctgg ataagggaat acgtatcctg tctgaactca
     1201 atctgggtct ggctttgttg ctcctggcgc tggtcctgtg tctgggacca accgtgcttc
     1261 tgctgaagtc atttgtggaa aatacgggcg gttatctttc ggaactggtg agtaaaacgt
     1321 tcaaccttta cgcgtatgag cccaagtcga gcaactggct ggggggctgg acattactgt
     1381 actggggatg gtggctttca tggtcgccgt ttgtggggat gttcatcgca cgggtctccc
     1441 gcgggcgaac cattcgcgag tttgtcaccg gcgtgctgtt tgttcccgcg ggttttacgc
     1501 taatgtggat gacggtgttt ggtaacagcg cgatctatct cattatgaac cagggggcca
     1561 cagacctcgc caatactgtt cagcaggatg tggcgctggc cctgtttaat ttcctggagc
     1621 atttcccgtt ctcttctgtg ctgtcattca ttgcaatggc gatggtcatc gtcttctttg
     1681 taacgtctgc tgattcgggg gcaatggttg tggatactct ggcatcaggt ggagtggcaa
     1741 acacacccgt ctggcagcga atattctggg cctcgctcat gggcattgtt gcaattgcgc
     1801 ttctccttgc cggagggcta agtgcgctgc aaacggtgac aatagcgagt gcattgccct
     1861 tctcagtgat cttactaata tctatatacg gacttttaaa agctttgcgc cgggatttga
     1921 ccaagcgtga aagcctgagc atggcgacaa ttgctcctac ggctgcacgt aacccaattc
     1981 cttggcagag aaggttacgc aatatcgcgt atctgccgaa gcgatctctt gtgaaacgtt
     2041 ttatggacga cgttatccag cccgccatga cgctggttca ggaggaactg aacaagcagg
     2101 ggacgataag ccacattagt gatgcagtcg acgatcgtat tcgtcttgaa gtcgatttgg
     2161 gcaacgagct gaattttata tatgaagtga ggcttcgcgg gtatatctca ccgaccttcg
     2221 cgctcgccgc aatggataat gatgagcagc agactgaaca acatcgatat tatcgcgctg
     2281 aggtttatct caaagaaggc ggtcaaaatt atgatgtgat gggctggaac caggaacagc
     2341 tgattaatga catactggac cagtacgaaa aacacctgca cttcctgcac ctggttcgtt
     2401 aatagcaaca tgccgtcctg ggggcggcaa ttattatccc ggccgcaata tgagggaatg
     2461 cagaatgatt tcacgctgga aatggatgct gaagcagaca attaaaaaac tatggttcag
     2521 ggcaacgtta ttcgcaattg tcgcgataat aacggccctt ttatcaattc tttttaaatc
     2581 aatgatacct gagtcggttt ccgtgaaggt tggtgcggaa gcagtcgata acattctgaa
     2641 catactggca tcgagtatgc tggcagtgac cacattttcg ctgagtatca tggtcacagc
     2701 ctacggttca gccactacta atgtgactcc cagagctacg cgtttagttg ttgaagacgt
     2761 caccacacaa aatgtactgg ccaccttcat cggttctttt ctcttcagtc tggtagggat
     2821 tattgccctc aatatgggag cttatggaga aagggggaga gtcattttat tcattgtcac
     2881 actggttgtc attgccttaa tcctcatcac attgcttcgc tggatacagc atttgacctc
     2941 tctggggagg gttggtgaga caacggcaca agtagaacag gcggccatcg aaacatttat
     3001 tgcgagagca agaaatccct gtctcggcgg atatccatgg cttgagaaca atgaacagcc
     3061 gaaaggaacg gttgcagttt atccgaagaa gattggctat gttgaatata ttgatatggt
     3121 gaaacttagc aagctgctga ccaatgatcc ccgtcatgta tacctcgtgg cgcagccagg
     3181 cagtttcata catccgtcca tgccagtttt gtacctgagt cagggccagg agtcgtcaat
     3241 cagcaccgat ttacttgaga cgattattgt ctcggatgca cgttcatttg ctcaggatcc
     3301 tcgattttgt cttagcgtca tggccgaaat agcctgccgg gccctttccc ctgcagtgaa
     3361 cgatcctgga accgccattg atgtcattgg cagaggtgtt cgtatacttt ccacttacgc
     3421 gcagaataaa tctgatgaaa tagaagtgaa atatccttca gtacatgttg caccacttca
     3481 gaataacgat ctactggaag actttttctc acctgtcgcg cgcgatggtg ccagtatgag
     3541 ggagattcag ataagagtcc ttaaaggact gtcgatgctg agtaaaggtt ggccagggat
     3601 atttgctgaa gccgcacata ccctggcatt tgaaacatta gagcatgcaa ctcgtgctga
     3661 ccatatagat tctgatagat atctaataaa atcaacttat tataatttat ttagtggcga
     3721 atattctaat aaaaaaacat agctgcagag caagcgattc ggttgttggc aagtcaaggg
     3781 caaagcattc gcgtgagaat gggcgtatgc tttgtgccaa aagcaaacgt tgctaattct
     3841 gcactgttca gcgatggcgc agtagcgaca ggcactgcca ctgtccagtc ggcaaaagcg
     3901 aacactttgc cactgcgggg ataaactgat tgaagctctc gcgcaaaatc acttattctt
     3961 ttgcgttaat aaaatgtagg agatgggtca tgacaggcga aaatcgtggc cggggaaggc
     4021 cgcgaaagac agaaagtacc tatgcagata ctcgtaatga tctgatccgc agtggccttg
     4081 aactgctgac gcagaatggt tttcttgcga cgggagtcga tgccattgtt aaaaatgcca
     4141 atgtccctaa aggctcattt tattactact ttaagagtaa agaggattat gctcagaccg
     4201 ttctgaacgc ctatgacagt ttcttcgagc ataaactcaa aaaacatcta catgaatcat
     4261 cctgcacacc aatggtacgt ctggaaaatt tcatcaatga tgcctgtgag gggattaaaa
     4321 aatacaattt tacccgcggc tgcctggttg gaaatatgat gcaggagagt ccggggcttc
     4381 cccaatcctt cattaaagta ctacagaata ttcttgagag ctggcaggca cttgttgcgg
     4441 cctgcctgtc cgacgcgtta tcttcaggcg aaataagttc aaatatgaat aatacacaac
     4501 tggcggctat tttctggagt ggctgggaag gtgcggtaat gcgttccaaa ctatattgtt
     4561 ctactgagcc agtgtacgac ttctggagct atttcaaaac ctcggtacgc tatcagtctt
     4621 ctcaggaagc aacaactggc cagtaaaagg ggccgcagcc cctaaaactt attatactaa
     4681 gcctgagctc ggcatccatc taattagcta tattgaatga ctgcacgacc gcgaattccg
     4741 ccactcagaa gtttttcagc cacagtgatg gcgtcactga gcggataaac ggtagtgaga
     4801 ctatccattc tggactctgg caaatactat accgcctgtt gccagattgc tgctcttttc
     4861 tctttcgcga acatgacgct atcaatccct ttcagcgtga caccacgcag aataaacgga
     4921 gccatattgc ccatcaaatc catcccctga gccatgccgc aggctgcaac aatactgcca
     4981 tatcggaccc cggcgcaggc atttctccag taacaggggg ttgtattgta ttttattgtg
     5041 ccggttcagg tgtgcgtttc ccggcgcgtc tgcaccggct taaccaaatt caacagggat
     5101 aaaatagtgc aaataaccga agtattactt gcagaatcgg gggaagcatc cggggtttta
     5161 ttcaacaggc tgtagatcac tgactgcgtc tgttggccgt ccacttcacg cgtcattcga
     5221 ccagagtaaa tattgatgat tactagattc gtgtatttta tcaggcattt tacttgcgaa
     5281 taaatcccct tgctatccag cgaaaccaca caggtagcta ttaccatatg tggtaataca
     5341 ctagttacag gaaaaatatg caagttccgc aatacgttgt gtgttgctcc ttagccagga
     5401 tcttttcttc catgcgagat atgacaacaa ttttgaggaa ggatgttcgt aactggtaga
     5461 gttactacaa caagcatggg aggctctgac tctggcagac attgtactgt ggaatgttgt
     5521 tacaggttgg ggcggcaacc gtgccaggag accatgagcc gttcacggca ttaaaggcca
     5581 cacttcagtc gtttacattc cctatggtgg caggcatctt ccattaaggc gtcagagcgt
     5641 taccaacgtt atgttgtcat ggctcatctt tcatagtcga tgcttatctg gtggctgaag
     5701 ctagccgcag ataaagagtg tgaagttgat accatggagt ggagtgacgt gcaggcagga
     5761 gattcgctct atgcagggtt gtgagatgaa ttcaaaccgg ctggcagcgc cagtgatatt
     5821 cgaagattcg tccggttgtt acccagtatg cattaaaaac ccagacatta tgggcaatat
     5881 aacggcttct acaacaagga agtgcctgtt taccatccgg gtgtaatgac tcacctttgt
     5941 gggcttttgc ccgtcgcgct cttaacgttg aagacattct ctgcctgccg attcaggact
     6001 gcgacaaaag ccatatctgg ctactggtca aggatgacca gcgtctggag taggtgataa
     6061 gccaaataga taagctggaa tatgtggtga aagtgcaacg taatcagtcc aatccgacga
     6121 tgtttaacaa gattgtggtg ttttttcagt aagtcattcg tcttctgaat caggtgtaat
     6181 gatctttaca gaaccattct ggcagtgcgt aacataatct gccggtaacg gacgatcgct
     6241 aatcagaacg tcaaatgcag agatcggtgc aaggctggca gaggccactt catcgaacaa
     6301 cgcgtggcgg gcgagcaaaa ttttccgtag tccacggttc atcgctttac gcttagtggc
     6361 aagatcttca gggttaaacc agctgacgcc aaagtgatta tgcacaccgc tggcggaaat
     6421 aaatattttt cgcggattga gagagtctaa tggcgaagag ttactggcat cgtaaaaagc
     6481 atcacttctg gcacgatacg taccaccaca aagtattgct gttacattag gcttttcatt
     6541 caacgcaaca aagacgcgat gtgagtaaca gatgccggtg aaggtgattg catccgggat
     6601 catgcttata acgagtggta tctcctggcc attatcaaag aagatcagat cattttcatt
     6661 aaccattccg gcagccagaa ttgcaatagg taagtcatca cgatgatttt ttggaacgtc
     6721 atggattact ggcatggatg gcgcgggttt attcaccatt acaatatagc cacccagtag
     6781 ggtcagtggc agaggttcat cttcctgatg gagatcgcga cgaatagtca ttacagaaac
     6841 ttccagcatt cgcgctgcgt ctttcaaatg gattctgtcg gttttcttaa gcagttccat
     6901 caaacgtcgg atacgctctt tttgcttcgt ttccatcaac tctctctccg taagaatgtt
     6961 ataaaaataa cattttggcg ttagtaaaca ttgttttcat taccatacta tacgaccgac
     7021 cctttgaaat gaaaagggta taggctgctg agttttgggc actaatcttg ttaggttgct
     7081 tatgattaga tagtgttggc ctgagtgatg agactggaag tctattaaat aaacatcggg
     7141 ttgatgtgct aatcgcgatc tcagtcacaa atgatgcaaa agtaacatgc taatgttatt
     7201 taaataccat tgttatgcga aagtttataa gaggtagtga aatggacatt gcagttattg
     7261 gttccaatat ggtggatctc attacttaca ctaatcagat gcctaaagaa ggagaaactc
     7321 tggaagcacc tgccttcaaa atcggatgtg gtgggaaagg tgctaatcaa gcggtggcgg
     7381 cggctaaatt gaattcaaaa gtcctgatgc tgactaaagt aggtgatgat atttttgcgg
     7441 ataatactat tcgtaatctt gaatcatggg gaattaatac cacttatgtt gagaaggtcc
     7501 cctgtaccag cagtggtgtt gcaccgatct ttgttaatgc caactcgagc aatagtatat
     7561 taataattaa gggagcgaat aaatttcttt ccccggaaga tatcgatcga gctgcagaag
     7621 atctgaaaaa atgcaaattg atcgttttac aactggaagt tcaactggaa acagtttacc
     7681 acgcgattga gtttggtaag ataaatggta ttgaagtgtt attaaatccg gctccggcat
     7741 tgcgtgagtt agatatgtct tatgcctgca aatgtgattt ctttatcccg aatgaaacag
     7801 aactggaaat attaaccggt atgtcggttg atacttatga tcatattcgt ttggccgcac
     7861 gttcgttagt tgataaaggg ctgaacaata ttattgttac catgagcgaa aaaggcgcat
     7921 tatggatgac ccgcgatcag gaagtccatg ttcctgcatt caaagtaaat gctgttgata
     7981 ctagtggcgc tggtgacgca tttattggtt gcttttccca ttattatgtt caaagcggtg
     8041 atgttgaagc cgctttgaaa aaagccgctc tctttgccgc tttcagcgtc acagggaaag
     8101 gtacgcaatc atcttatccg agtattgaac aatttaatga atttttaacc ttaaacgaat
     8161 aatcatcctg cacagttaaa ggtagcacta tgaacgataa aaacatcatt cagatgcctg
     8221 atggatatct gaataagact cccttgttcc agtttatttt gttatcgtgt ttattcccgc
     8281 tatggggatg cgcagctgca ttaaatgaca tattaatcac tcaattcaaa agtgtatttt
     8341 cgctaagtaa ctttgcttca gcattggtgc agagcgcatt ttatggaggt tattttttaa
     8401 ttgcaatacc agcatccctt gtgattaaaa agaccagtta taaggtggca attttaattg
     8461 gcctgacgct gtatattggt ggttgtacac tttttttccc tgcctcgcat atggcaacct
     8521 acactatgtt ccttgcagct atttttgcga ttgcgattgg cctgagtttc cttgaaactg
     8581 cagcgaacac atacagttca atgatcggtc caaaagccta tgccacattg cggctcaata
     8641 tcagccagac cttttatccg attggtgctg cgtctggtat tttattgggc aaatatctgg
     8701 ttttctccga gggggaaagc cttgagaagc agatgtccgg aatgaacgcc gagcaaattc
     8761 acaactttaa ggtgctgatg ttagagaaca cccttgaacc gtataagtat atgattatga
     8821 ttctggttgt ggtcatggtg ttgttccttt taacccgctt cccaacctgt aaagtggcac
     8881 agacaagtca ccataaacgc ccgtcagcaa tggatacgct gcgctatctg gctaaaaatt
     8941 cgcgctttcg tcgtggtatt gtggctcaat tcctttatgt gggtatgcag gtcgcagtat
     9001 ggtcatttac catccgtctg gcactggagt tgggcgatat caacgaacgt gatgcttcaa
     9061 acttcatggt ctatagcttt gcctgcttct ttattggcaa gtttatagcc aacatcctta
     9121 tgacgcgttt taatccggaa aaagtattga ttctctattc cgttataggg gcgctgttcc
     9181 tggcatatgt tgcactggct ccaagtttca gcgcggtcta tgtcgccgtc ctggtaagtg
     9241 ttttgtttgg cccttgctgg gcgacgatct atgcaggaac actggataca gttgataacg
     9301 aacatacaga aatggcagga gcagtcattg ttatggctat tgtcggcgcc gcggtggttc
     9361 cggcaattca gggatatatt gcagatatgt tccactcact tcagctttca ttcctggttt
     9421 ctatgctctg ctttgtctat gtcggagtct acttctggcg tgaaagcaaa gtacgtaatg
     9481 cactggctga agtcacagag tcctgaggag aatcgttatg agtacgcgta tttatttgtg
     9541 gcgggcgctt tttggcgaaa aacctcgtat cttgctggaa aatagtgatt ttactgtcac
     9601 gtcttttcgc tatgacagtg gtgtcgaggg gcttaaaatt gcaaacagtc gtggacattt
     9661 aattattctt ccctggatgg ggcagatgat ttgggatgcg caatttgatg gacacagtct
     9721 caccatgtgc aatatgtttc gccagcctaa accggcgaca gaggtgatag aaacctatgg
     9781 ctgttttgcc tttcattcag gtttgctggc taatggctgc ccatctgcgg aagatactca
     9841 tctgctacac ggtgaaatgg cctgtgccgc gatggatgaa gcatggatgg aattagaggg
     9901 agatatgtta cgccttaccg ggcgttgcga gtatgtaatg gggttcgggc atcattatct
     9961 ggctcagcca acagtggtcc ttcacaaatc ctctacattg ttcgatatta agatggccgt
    10021 aaccaatctg gcttctgtag atatgccact acaatatatg tgccacatga attatgcata
    10081 tataccaaat gctacgttca gccagaatat accggacgaa atattaaggt tgcgtgaatc
    10141 agtaccttcg catgttaatc ccaccgctca gtggctggcg tttaatcaac ggattatgca
    10201 gggagaagcc tctctgtcga cgctgaatca accggaattt tatgatccag aaatagtctt
    10261 ctttgcagac aaactggacg cttacacaga tcagccagag tttcgcatga tcgctcccga
    10321 tggcactacg tttgttactc gtttctccag tgccgaacta aattatgtga cacgctggat
    10381 cctttataac ggcgagcaac aggtagccgc ctttgcatta cctgcaacct gtcggcctga
    10441 ggggtatctt gccgcgcaac gtaatggcac gctgattcag gttgcaccgc agcaaacacg
    10501 cacttttaca gtcaccacag gaattgaata accacttaag gcctgaacag cggcgcgctt
    10561 atcgttaatg taagcgcgtt tttttacccg ccaggacaat actatgatag cgttgccctt
    10621 atagacgatc acattatcgt ccactccaaa tttgcgcaac tactgtagag tggtgtactc
    10681 cacagcttct tttaggatct caacttccat tgttttctta ccgagcaagc actgcaactc
    10741 ttttatttat ttgtagtggt caacaaaaac tggtcacaat ttcagatttt ttccagtatc
    10801 tattttccga ttcgttcggc ggtaatccac cgttatattc atgaggccgg agtgcactat
    10861 aaaagtgtat gcgtaaaccg actgctgtat gtagccatta agcctgtatt ggtaacatag
    10921 gtacccacct tttcaacaag tggacacctg ttatagaaca aaaaacatta tcagctgaac
    10981 cccgcaggtc attttcaaat gagtttaaac ttcaaatggt tacactggct ttacaaccaa
    11041 ggggttctgt tgcccgtatt gcccgggagc atgatatcaa tgataatctt ctgttcaaat
    11101 ggctcaggct ctcgctgatc aaagggcgta tatcacggcg tcttccgtta acgaactctt
    11161 ctggcattgg cgttgaatta ttacctgtgg agatgacgct gggatgagcg gccttacccc
    11221 gttttttacg cgaggtaatt atgaagatca atgactagcc gtcgaaccgt gtgcatgaac
    11281 tgctgcactg aaacttctca gctgtaaaat aatccttacg ctacgtactt ctcggagcgc
    11341 ttacgattaa tcggatgcag tcctgactgg aagaaagaaa aatggagaaa ataagaggaa
    11401 ttaatatcct ccacgttcct gaaatcctgg aggatattaa tgtcatcaaa atattagcga
    11461 acgataatcc ctaacaaaat acccagtgcg ccaaagtcag catcactgaa ggtggtgtta
    11521 gcaaaaccaa tatcacccag caccggcaat aagaatactg gcaggaaggt aataagcaag
    11581 ccttgagcga aggcacccaa aatcgcacca cgacgtccac ccgttgcatt accgaatacc
    11641 cctgcagctg cacctacgaa gaagtgcggc accacaccag gaataatcac gatcatgttc
    11701 agcagataca acgtgaacat accgattaaa cctgcagcaa agctactcag gaaaccaacc
    11761 agtacagcgt taggtgcgta agggaagacg accgggcaat cgagagcagg tctggcattc
    11821 ggaaccagct tgtctgaaat acctttgaat gccgggacaa tctccgcaat caccatgcgc
    11881 acaccctgca gaatgatgta cacgccagct gcgaaggtaa tggattgcat gatagagaac
    11941 atgaaccagt ttttaccacc gctaagctct ttcaccgcat ctgcacccgc aaacaggcac
    12001 gtcaccagga aaattatgct catggtaaaa gagatagcaa ccggcgtgtc gcgcaagaac
    12061 agcagacttt tcggcacatt catgtcttca gtcgagtgct ctttattacc aaacttgctg
    12121 ccgatgaaac cggccaatac ataagataat gttgagaagt ggccgatggc aacgtcatca
    12181 gaaccggtca ctttcttcat gtacggatgc gcaatagccg ggaaaaagac cattgccacg
    12241 ccaacaacca aagagccaac ggctataagc gtaatgccgg tcatgcctgc tgttgccaga
    12301 attaccgcta ccatcatcga cataaacaac gtgtgatgac cggtcaggaa gataaatttc
    12361 cacggtgtga aacgggcaat cataatgttg ataaccatcg caaagaacat aatcatcgcc
    12421 atttctttcc cgaagctttt ctgcgctacg gaaacaatgg cttcattgtt cggcactaca
    12481 ccctgaatac caaaggcatg ctggaaaatg tttgcgaagt cacccagtga cgacacaact
    12541 aaacccgccc ctgcgcctaa aatcacaaac cccataatgg tcttgacggt acctttaatg
    12601 cattcggtta ccggtttttt ctgtgcaatc aggccaatca gggcgattaa accgaccagc
    12661 actgcgggtt cggaaagtac atcgctcatc aaaaaacgaa agaaatccat atcgccccca
    12721 gcttacagtg cgcctaattc gtttaatacc acggataaac gttctctcat ggctttctta
    12781 tcaatcatat tttccagtgc gacgattttg cctgcgacag actgcgcgac taactgctcg
    12841 gcaatgtctt tggtgccgac atagatatca ctcgacgtcc ctttcgcgga gcccaggtcg
    12901 acgtgatcaa catcagcgct gacggacaaa tccttcaaaa tgcttttaat gctcatttcc
    12961 atcatcaggc tggtgcctaa gccgttaccg caaaccactg taattttcat aaatgttcct
    13021 ctggattaat agcgcgcaat aacagacaaa atgtcctgcg tcgttttggc catcaaaatg
    13081 gcctgtatgt ctttttcgtt atcgaataac ttggcgagtt gagaaatcgc ttcaatgtgg
    13141 ctggtactgt cggttgcagc cagcacaatc agtaatttca ctggatcgtt ttcatcggca
    13201 tcaaaattca cacctgaagg gatcagggtc agtgctaatg acagcttgtt agctccctct
    13261 tccgggcgcg catgaggcat cgcaatacca gggccaacca catagtacgg accgatagtg
    13321 tcgtgcgagc gatagatcgc atcaacataa cgcggttcga tagaaccgtt ttcaatcaac
    13381 ggccggcatg aaatcgccac ggcttcccgc cagtctttgg cctgttccac gacctgaacg
    13441 acctctgtat ttaatagatt ttttaacatg ttactgataa ctcccgatga taattaacag
    13501 gagagtagat attttacgca ttaattaatg tgatgattgt cgcatttgat agcgctatct
    13561 gataacgcta tcaatctttg tgatagcgct atcatttctc tgccgccatg ctttggctat
    13621 attcggttcc tgcatataca atgcaacagg atatttgcta ccgtgggcaa aaactggaaa
    13681 gagtaatggt gaaataaaag taatgaaaac agctggttgt aattatccat ggagtcgaaa
    13741 tggaaaaaac acgtaagcgc cgtggtaccg gacgggtgac gctgcaggag gtcgctaact
    13801 ttgcaggtgt cggaaccatg accgtttccc gtgcgctgag aacaccggaa caggtatctg
    13861 acaagctgag ggaaaagata gaacaggccg tagaagagtt gggttatatt cccaacagaa
    13921 ctgctggcgc ccttgcatct ggtcacagtg atacagtcgc cgtactggtt ccttccctga
    13981 cagataaagc cagctcccgc tttatgcagt cgttgcagca ggtgcttaat aaaaatgaat
    14041 tccagctact gcttgggtgt catgagtata atcagctcaa agaagctgaa attctgatga
    14101 cgcttcttca aggcaacccg gcggcgttag ttatttttgg ttctcagttg gctgataaaa
    14161 ctcaccagat actcgagaaa acaaatatac ctaccattaa cgttgtcggt tcccctttca
    14221 gtccggctca aattaccatc gaaacagcat tttttgaagc atcacataaa ctgaccgaac
    14281 atctgctgga acagggttat aagaatatcg gatttatcgg tgcgcatatg gacaaccgcc
    14341 tgcaacgtca gcaactgaac ggctggcata aagcaatgtt gagccattac cagaactctg
    14401 atcttgttat caccatgccg gatgccgcaa gtctgcaatt ggggcgttac gcattaaacg
    14461 agatattact gcgacaacca gagctggatg ctgtgatttg cagccacgaa gagattgcgc
    14521 tgggtatcat gtttgagtgc cagcggcgtt tgttaaaaat tcctgaaaat atcgctgtag
    14581 cttgtcttga tggttcagac aattgcgatc aaacacaccc aaccttgaca tccatccgta
    14641 ttgactacaa aaaaatgggg atagagacgg gtaagttact gattgggcta ttgaataata
    14701 atcatgatga atcagaagag tcgcgtatcg tgcaattcaa ctaccagata gaattacgtc
    14761 agagtacatg agcctaaaag ggcactatta caaagttagc tattgtggta gggatagccg
    14821 tttccggcta ttccccgcgc agatccggat gtatgctgct aacgcatcca ttcctacttc
    14881 aggtatttgg cgtagaagcg gatgccaaaa acttgccgca aaaccgcaat atcagcctgc
    14941 agagtcgctc tccagattat ccattcctat attatctctg caccggatga attacgcgaa
    15001 ccactcagaa atatgacacg tatgcaactt atcaggactc cgggatcctg gaagcctagt
    15061 gccagtgaat atcgtaatgt taccaacgtt tatcgcattg cattgaaatc acttgcctga
    15121 cgctatttcg agttacatga tgaaatcgcc gaccttgatg tcatgattgc ggcaattgtc
    15181 gatgaactgg cacctgaact gattaaacgt aatgctgttg gatacgaaag cgcttcgcag
    15241 ttgctcatca ctgccggaga taatccccaa cggctaagaa tagaatcagg ttttgcggtg
    15301 ctgtgtggtg tcaactcggt taccgtatct tctaaaaaaa tgaaccgtta ccgacttaat
    15361 cggggtgggg agcgtgctgc aaatagtgca cttcagatca tcgctatcgg acgtttacga
    15421 actgtaagcg tgcagcaaga accgtattga cggggatgtg ttattcagtc ggcagtgcta
    15481 cgcgccaggg gagcagttcg ccgacccggt ttatcggcca gtcggctatg acgtcaagga
    15541 catagcggag gtagctttct ggctccactc cgttcagttt gcacgtcccg atcaggctgt
    15601 acagcagcgc tccccgctct cctccatgat ccgaaccgaa gaacaggtag atggtatgac
    15661 ctttatatac tcactatagc attggtggga tattccagag gaggtcatta tgcattcgga
    15721 aaatattgca gcttacatcg ggctggatgt acataaagaa acgttggcgg ttgctattgc
    15781 tgcacctgaa cggcttggcg aagttcgtta ttatggtacg attaacaacg aagcccaggc
    15841 tgtacgtcga ttatttcaga aattgcaggg actttacgga aacattcttt cctgctatga
    15901 ggctggcccc tgtggttttg ggctatatca tcaactcaca gcgatgaata taaaattcca
    15961 ggttattgcc ccttcccgga taccaaaatc accgacagac agaatcaaaa atgatcatcg
    16021 ggacgcaatt tctcttgcca ggttactgcg ggctggtgaa cttacccctg tctggatccc
    16081 tgacctgact catgaagcta tgcgggatct aatcagagcc cgtgcagcag ctaaacgaga
    16141 cagcagagtt gcccgacagc gaattctgag catgctattg cgaacagaca aacgttatgc
    16201 agggaagcac tggaccggca aacatcgaac ctggttagct aatcaatcat ttagtcagcc
    16261 atctcagcag attgcttttc aacactattg tcaaagtctg gagcagattg aagatcgcat
    16321 acttcaactc gaccaggaaa tatctcgcct gttacctgag tggtctctgt gcaatctggt
    16381 ctgccagttg caggctctaa aaggtgttgg tcagctaatt gcaataaccc tggttgctga
    16441 actgggtgat ttttcgcgat tttccaatcc caaacagctc atggcttttc tgggactggt
    16501 gccaggtgaa tattccagtg gaaacagtat tcgtcccaga ggaataacaa aagttggaaa
    16561 cagcgaactg agacgtctgc tttacgaagc cgcctggtct tatcgtacac ctgcaaaagt
    16621 tggtgcatgg cttatatatt accgaccgga ctctgtaaca caatattcca aagatattgc
    16681 atggaaagct caacaacgat tgtgttctcg ttaccgaact ctgacagcaa aagggaaaaa
    16741 atcacaagta gccattacgg cggtggctcg tgagttgact ggatttatgt gggatattgc
    16801 acttgctgcc caatcatcat tcagtcagca gaagcaaaat taaaccctga agcagtcaga
    16861 cacggaatga gtgatcctcg aaaaagaatt cagggaagcc agaatgcgct aatacctgca
    16921 cgtagaaaga ggccagctca ctgcgtaaat cgtcatgggg ctaaaatccc cgaataagag
    16981 tttgtaaaat ccaagataca gtctgactgt ttcaggttct ttaatccgcg atataacgaa
    17041 gctcccggag ctgcgtaggt ctttttgggc ttaatatttg acaaaggtca taagagtttt
    17101 tgcggcccag actgaccatc cgcaacgcat tttcagcgat gttattgtcc gcctcagccc
    17161 agccatcatc tgcatagtac gtcagcgccg gccactggtt cagggcgtat gcgaacgctt
    17221 tcgccagttc tgagtgtcgc gacagggttt tcatcttttc acgcagccag ctttccaggg
    17281 atttcagcag cggtttcgtt ttcaactgac gttcggcaag gcgctgctcc gccgtcattc
    17341 cccttatctc tgcctcgatg gcgtacagtt cgccgatccg tttcagcgct tcctccgtca
    17401 gggctgacgg ggtgcgaacg tgcacatcgt ggattttacg gcgggcgtga gcccaacagg
    17461 cggcttccgt tatccggcca tcccggtaca gctcgttgaa cccggcgtat gcatccgcct
    17521 gcagtacacc actgaacccc gcaagatggg tctgcggatg gatgcctttt ctgtccgggc
    17581 tgtaagcgaa ccacaccgcc ggcgccagcg ttgacccggc gttacggtcg tcacgaacgt
    17641 aggtccataa ccgcccggtc ttcgttttct tattgcctgg caacagcacc gggacaggcg
    17701 tgtcatcagc atggagctta ccgtcagtca gcacatagtc ctgaagcgct tcttccagcg
    17761 gtgacagtag ccggcagcat gcatccaccc agcccgacag cagtgaacga ctcagctcca
    17821 cgccctggcg gccgtacatt tcagactggc ggtacagcgg ggtgtgctct gcatactttg
    17881 agatcagcac gcgggccagc agccccggtc ctgcgatacc ccgctcgatg ggccgtgaag
    17941 gcgcgggggc ctgcacgatg gcatcgcact gagtacaggc atgcttttca cgtacagtcc
    18001 ggataacccg gaaggcgctg cgcatcaact ccagctgttc ggcggcatcc tcacccagat
    18061 agctcagcga gcctccacat tccgggcagc atgacgctgc cggcagcagc cgtttttcat
    18121 cgcgggggag tgattcgggg aacggtttgc gggtgcgggt ttgacgcagc gggcgctgca
    18181 cggccgggtc gtcaacccga ccggtaaggg tatcactttc tttctgaagt gccttcaggt
    18241 cagcttccat ctgtgcgata cgacgggaga ctttttccga gcggctgccg aagttcatcc
    18301 ggcgcagctt atccagctgt gcctgcagat ggtctatttc gcgttcacgc tcgttcagct
    18361 tttccagcag ggcacggttc agcgcctcct gttcggcaag gagacgtttc agtgcattga
    18421 tatcgtcagg aagtgagctg ctcataccgg gtatattacc aggctcattc agcgtcgacc
    18481 aggataaaga ggcttacaac atagtcaggg acgtaagtag tcttttaggc tgccgccagt
    18541 cgataccttc cagcagcatc gccagctgtg ccggtgtgag gaacactttg ccatcccggg
    18601 ctgacggcca ggcgaagcgg ccgcgctcca gccgtttggt cagcagacac agtccatcgc
    18661 cggtagacca gaggagcttt acctgactgc cattacgccc acggaagata aaaacgtgac
    18721 ctgacatcgg atcgtctttc agcgtcgtct gcacctttgc cgccaggccg ttgaagccgt
    18781 ttctcatatc ggtgatgcca gcgaccagcc agatctttgt ccctgatggt aatgggatca
    18841 acgtttgagc tcccgtatca gcaggttcag gatattttcg ctgatggcac cattcagacg
    18901 gagtgatccg tgccggaacg tcacttcaca gctgatattg agtgattcag gtgtcgcagc
    18961 agctacaggt tgtgaccggg gggaggttgt agggacaaca tcttcggcat caagtgttat
    19021 cgggagcagc tcaggcacgt tgttttctgt tgttgaagga ggacgtagtt ttccttcgcg
    19081 ccagtactgg cgccacttga acagcaaatt atcgttgatc ccatgctcac gagcgagttg
    19141 cgctacggag atctctggtc gatgcgagag ttcaaccatt ttgattttga actcaacggg
    19201 ataattaggg ctttttttac gcactgcggt taatgatttc atggatagcg tccaccatat
    19261 ttggtgtcca ctattctctc aggaatttca ggatctgcca gacggtgctg agacgacgct
    19321 tactgccgta gataactgaa gtaacagatc tgctgctgtt gtgaagcgaa ctttgatacc
    19381 tgtacggact gcttcatagc ccatcgctat tgcaagatgg gttttcccca cacctgatgg
    19441 ccccagtaat acgatatttt cattacgttc tatgaagctg agtgagcgta acgactggag
    19501 ttgcttctgc ggtgctccgg tggcgaatgt gaagtcatac tcttcgaacg ttttcaccgc
    19561 cgggaaggct gccattcggg tatacatcgc ctgtttacgt tgatgacgtg ccagtttttc
    19621 ttcatgaagc agatgctcca ggaagtccat ataactccat tcctggtcta ctgcctgttg
    19681 tgacagcgca ggcgctgcgc ttataaggct ttccagttgc aactgcccgg cgagcgccat
    19741 cagtcgttga tgttgcagtt ccatcatcac gccactcctc agcagaatga gtcgtagatg
    19801 gagagtggat gatgcagggg gtgtttgtcg aagttcacca gattttcatc aagatgcacg
    19861 tcatactctt ttttctccgg aggcagtgcc agcatggact gctgctcttc gagccagcga
    19921 tcgcagggac gtgcctggat tgtttcatgc tttcgttgaa cgcgcgatcg atggtcttca
    19981 ctccatcgct tgttactggc atgccattca cgagcaatgg tttcgaaagt atatgcccct
    20041 gaattctcgg cctgagcttc tttctgttcg gcttttggat caatgccctg cactaaaagc
    20101 tttttagctt cgtcgcgctt tgctcttacc tgagcaagcg tcacagtagg ccaaacacca
    20161 aaagcgaggc gatcctcttt tttgtctgag gggcgtctgt atttcatgcg ccagtattta
    20221 gaacccttgg ccgaaacctc aagatacaga cctccgccat cggccatttt ataggttttg
    20281 tcttttggct ttgcggtctc gatctgtctg gcgttgagct tcatttgggg gcacattcct
    20341 aatcgaagtt aagatgcccc caattatgcc cccaatgaca tccggatttc aacggactat
    20401 ctcggacgac tcaggacgta aaaatcgctg caagcattga ttttaaaagg ataattggac
    20461 tttttcggat ggtcttggaa gtactaatgg tgccgaaggc cggactcgaa ccggcacgta
    20521 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
    20581 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
    20641 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
    20701 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
    20761 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcaggcggcg aactggaagt
    20821 ttatgagtac gatcccggtg aactgaagcc acaagatgtt gaagtgcagg tggattactg
    20881 cgggatctgc cattccgatc tgtcgatgat cgataacgaa tggggatttt cacaatatcc
    20941 gctggttgcc gggcatgagg tgattggtcg cgtggtggcg ctcgggagcg ccgcgcagga
    21001 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
    21061 ttgcgatgcc tgtattagcg gtaatcagat caactgtgag caaggtgcgg tgccgacgat
    21121 tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg tgattccact
    21181 gccagaaaat attgatatcg agtccgccgg gccgctgttg tgcggtggta tcacggtctt
    21241 caaaccactg ttaatgcacc atatcactgc caccagccgc gttggggtaa ttggtattgg
    21301 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
    21361 tagttcgaat ccggcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
    21421 tagccgcgat ccgcaggcac tgaaagcact ggcggggcag tttgatctca ttatcaacac
    21481 cgtcaacgtc agcctcgact ggcagcctta ttttgaagcg ctgacgtacg gcggtaattt
    21541 ccatacggtc ggtgcggttc tcacgccgct gtctgttccg gcctttacgt taattgcggg
    21601 cgaccgcagc gtctctggtt ctgctaccgg cacgccttat gagttgcgta agctgatgcg
    21661 ctttgccgcc cgcagcaagg ttgcgccgac taccgaattg ttcccgatgt cgaaaattaa
    21721 cgacgccatt cagcatgtac gcgatggtaa agcgcgctac cgagtggtcc tgaaagccga
    21781 cttctgatcc ctttgccggg tgcctgtcgc ccggcactta aatctctttg gaaggcgtct
    21841 cgcaatctgc tgaatttagc ggtgaagagt gcgcagccgt tgcctatact ccaggcagga
    21901 aactggagga aatctcatga gcgaacccct gttaattgcc cgcacgccgg acacagaact
    21961 gtttttactg ccgggaatgg cgaaccgtca cgggctgatt accggcgcaa cggggacggg
    22021 taaaaccgtt acgctgcaaa aactggcaga gtcattgtcg gaaatcggcg tgccggtgtt
    22081 tatggctgat gtgaaaggcg atctgaccgg tatcgcgcag gcaggaacgg cgtcggaaaa
    22141 actgctcgca aggcttaaaa atatcggcgt caatgattgg caaccgcatg ccaatccggt
    22201 ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg cgggcgacgg tttcggatct
    22261 gggaccgctg ttgctggcgc gactgttgaa tctcaacgat gtgcaatctg gcgtgctgga
    22321 tatcattttc cgcattgctg acgatcaggg gctgttactg ctcgacttta aagatctgcg
    22381 ggcaattacc cagtacatcg gcgataacgc caaatccttc cagaatcagt acggaaatat
    22441 cagtagcgca tcggttggtg ccatccagcg cgggctgttg tcgctggaac agcaaggcgc
    22501 agcacacttc tttggtgagc cgatgctgga tatcaaagac tggatgcgca ccgatgccaa
    22561 cggtaaaggc gttatcaata tcctcagcgc cgagaaactt tatcagatgc cgaaactgta
    22621 cgccgccagc ctgctgtgga tgctttcaga gttgtatgaa caattgccgg aagcgggcga
    22681 tctggagaaa ccaaaactgg tgtttttctt cgacgaggca catctgctat ttaacgatgc
    22741 cccgcaggta ctgctggata agattgagca ggtgataagg cttattcgct caaaaggcgt
    22801 gggcgtctgg ttcgtttcgc aaaacccgtc tgatattcca gataatgtgc tcggccagct
    22861 cggtaatcgc gttcaacacg ctttgcgtgc ttttacgccc aaagatcaga aagcggtaaa
    22921 agctgcggcg caaaccatgc gccccacccc tgcatttgat acggaaaagg caatccagga
    22981 actggggacc ggcgaggcgt taatctcgtt tctcgatgca aaaggaagtc cttctgtggt
    23041 ggagcgggcg atggtgattg cgccttgttc gcggatggga ccggtgacgg aagatgagcg
    23101 taatggcttg attaatcact ctccggtgta tggcaagtac gaggatgacg tggaccggga
    23161 atccgcctat gagatgttgc aaaaaggcgt acaggcaagc accgagcagc aaaataatcc
    23221 ccccgcgaaa gggaaagagg tagcggtaga tgacggcatt cttggtggat tgaaggatat
    23281 tttgttcggc actaccggac cacgcggcgg gaagaaagat ggtgttgtgc aaacaatggc
    23341 gaaaagcgcc gcccgccagg tgacgaatca gattgtgcgt ggaatgttgg ggagtttgct
    23401 gggggggaga aaaaggtaag ccaggtgcga tttgtctttg cctgatgcga cgctggcgcg
    23461 tcttattatg cccacaccac tgcaatatat tgaattttaa tgatttttca taaatgaacg
    23521 aagcacacta tgctaacaat aataaaggga gctttcgctc cctttattcg ttcattcggt
    23581 tacgattttc tcattaacag ccacaggctg attaagaaga agctagcgct tggcaacagt
    23641 gcgccgatga tcggcgggat gccataaacc aacgtcagcg ggccgaagat ctggtccagt
    23701 acgtagaaga caaaaccgaa gctaataccg gtgaccacac gcacacccat cggtacgcta
    23761 cgcagtgggc caaagatgaa cgacagcgcc atcagcatca tcaccgccac ggatagcggc
    23821 tggaagattt tgctccacat gttgagctga taacgcccgg catcctgacc gctcgacttc
    23881 agatacttca cgtagttgtg caaaccactg atggagagtg catccgggtc cagcgccacc
    23941 acgcccagtt tgtccggcgt gaggttggtt ttccaggtgc cgctcaccgt ctgcgaaccg
    24001 gtgatctgtt tcggattggt cagatcagat tcatcaacct gcgacagacg ccagacttta
    24061 tgttccgggt caaacttcgc ggtagcggca tagcgtacgg attgcagacg acgattctcg
    24121 ttaaaggcat aaatactgat gccacctaac tcttcgtcac ctttaacccg ctcaatgtag
    24181 acgaagttgt tgccatcttt cgcccataaa ccttgctggg tagagagcaa cgagccgccg
    24241 tacatcgcct gcgcacggta gttacgcgcc atctgctcgc cctgcggcgc gacccattca
    24301 ccaatcgcca tcgtcagtaa gaccagcgga atggcggttt tcatcaccga cagcgccacc
    24361 tgcatacggg taaaaccaga agcctgcatc accaccagtt cgctgcgctg cgccagcatc
    24421 ccaagaccaa gcaacgcccc aagcagagcc gccatcggga agaagatctg cacatctttc
    24481 ggcacgctca gcaaggtata cattcctgcg cctaacgcgt cgtaactccc ctgcccggct
    24541 tttttcaact gatcgacaaa tttgataatg cccgacagcg acaccagcat gaacagtgtc
    24601 atcatgatgg tggtgaaaat agttttaccg atatagcggt caagtacgcc aaaaggttgc
    24661 atcacaccgc tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc
    24721 aaaggttgag aacaatcgcc aaagccagat aaatcaggtt aacggtccac atccacagcg
    24781 tcgggtccag cttaccttta ccgccgttcg atttcaggga ggtctggatc aggaagaaca
    24841 acagatacag cagcatggct ggcagcatcg acagtacgcg tccctgacgt gggttaacca
    24901 cgctcagcgg tacgaccata agtgccatca taaacacggt gaataccaac gtgatacgcc
    24961 agttcagttc tgcgcgagca cgatcggtgt cagtgttcca caatgtgcgc atgtccatct
    25021 ggtcggtatc gttcgggtcg agcaccaccg cctggtgacc aatgatcgcc tgataatcct
    25081 ggaaatccgt aatgcggaaa tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt
    25141 tgagagtgac gacctgggag ccgtcgcgca gctgggttaa atgtccggaa tcggccacca
    25201 ccacagaagg acgtgcatta ccttttggtc gaatttgcgc gaggaacaca tctttgaaat
    25261 cgctgccgtc aacgctttcg atgaacagca ccgagctgcc attagtcgct tgctggaatt
    25321 gcccttgcgc cagcgccgcc atgccagggt tcgctttcgc ttctgctaac acttcatcct
    25381 gatgacgcga tgaccacggt cccgcccaca tcacgttaac cgccgcgacg attgccgtga
    25441 ataccgcaag gatcattgcc gctttcacca gaaccgcttt gctcaggccg caggcatgca
    25501 ttaccgtaat ttcactttcg gtatacagtt tgcccagcgt catcagcagc ccgaggaaca
    25561 ggcttaatgg caggataagc tgcgccattt ccggcacgcc caacccgaga agggagagca
    25621 ccagattcgc cggaatatcg ccgtcaaccg ctgcgccgag gatcctcact aacttttgac
    25681 agaagaagat caaaagcaag atgaagagta tcgccagctg gcttttgagc gtctcccgca
    25741 ccagatatct tatgattatc actttaaata cgcccgtaaa aactcgtctt ttgcaggatt
    25801 ttagcttgtt tcatggctta aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa
    25861 gataattata ctcaacggat tcacctctca gattttgttc tgacgtgcca atgccgtaat
    25921 aacgttaaga ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa agcttgcaat
    25981 tctatctgta gccaccgccg ttgtctttaa gattcaggag cgtagtgcat ggagtttagt
    26041 gtaaaaagcg gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa
    26101 ccacgtcgcc tttctccgat tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc
    26161 gccctgctac gtcggggcga actggaagga aaaccggggc agacattgtt gctgcaccat
    26221 gttccgaatg tactttccga gcg
//
LOCUS       DACAPB010000050        25977 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.050, whole genome shotgun sequence.
ACCESSION   DACAPB010000050 DACAPB010000000
VERSION     DACAPB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.050"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..924
                     /locus_tag="IT904_001853"
     CDS             <1..924
                     /locus_tag="IT904_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAP3191640.1"
                     /translation="TMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGNE
                     LTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEGA
                     LSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDEK
                     AVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLAP
                     LFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVAP
                     FGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            938..2218
                     /gene="gabT"
                     /locus_tag="IT904_001854"
     CDS             938..2218
                     /gene="gabT"
                     /locus_tag="IT904_001854"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAP3191641.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            2456..3856
                     /gene="gabP"
                     /locus_tag="IT904_001855"
     CDS             2456..3856
                     /gene="gabP"
                     /locus_tag="IT904_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAP3191642.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            3877..4539
                     /gene="csiR"
                     /locus_tag="IT904_001856"
     CDS             3877..4539
                     /gene="csiR"
                     /locus_tag="IT904_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAP3191643.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSQQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(4540..4989)
                     /gene="kbp"
                     /locus_tag="IT904_001857"
     CDS             complement(4540..4989)
                     /gene="kbp"
                     /locus_tag="IT904_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAP3191644.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(5073..5231)
                     /locus_tag="IT904_001858"
     CDS             complement(5073..5231)
                     /locus_tag="IT904_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAP3191645.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            5414..5713
                     /locus_tag="IT904_001859"
     CDS             5414..5713
                     /locus_tag="IT904_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3191646.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            5723..6247
                     /gene="ygaP"
                     /locus_tag="IT904_001860"
     CDS             5723..6247
                     /gene="ygaP"
                     /locus_tag="IT904_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAP3191647.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(6294..6698)
                     /gene="stpA"
                     /locus_tag="IT904_001861"
     CDS             complement(6294..6698)
                     /gene="stpA"
                     /locus_tag="IT904_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAP3191648.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLL"
     gene            7366..7815
                     /gene="alaE"
                     /locus_tag="IT904_001862"
     CDS             7366..7815
                     /gene="alaE"
                     /locus_tag="IT904_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAP3191649.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(7852..8196)
                     /locus_tag="IT904_001863"
     CDS             complement(7852..8196)
                     /locus_tag="IT904_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAP3191650.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            8348..8677
                     /locus_tag="IT904_001864"
     CDS             8348..8677
                     /locus_tag="IT904_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAP3191651.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            8925..9170
                     /gene="nrdH"
                     /locus_tag="IT904_001865"
     CDS             8925..9170
                     /gene="nrdH"
                     /locus_tag="IT904_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP3191652.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            9167..9577
                     /gene="nrdI"
                     /locus_tag="IT904_001866"
     CDS             9167..9577
                     /gene="nrdI"
                     /locus_tag="IT904_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP3191653.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            9550..11694
                     /gene="nrdE"
                     /locus_tag="IT904_001867"
     CDS             9550..11694
                     /gene="nrdE"
                     /locus_tag="IT904_001867"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP3191654.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHANTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTRTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYNNHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            11704..12663
                     /locus_tag="IT904_001868"
     CDS             11704..12663
                     /locus_tag="IT904_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 2 subunit
                     beta"
                     /protein_id="HAP3191655.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            13018..14220
                     /gene="proV"
                     /locus_tag="IT904_001869"
     CDS             13018..14220
                     /gene="proV"
                     /locus_tag="IT904_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAP3191656.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKAIDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            14213..15277
                     /gene="proW"
                     /locus_tag="IT904_001870"
     CDS             14213..15277
                     /gene="proW"
                     /locus_tag="IT904_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAP3191657.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            15334..16326
                     /gene="proX"
                     /locus_tag="IT904_001871"
     CDS             15334..16326
                     /gene="proX"
                     /locus_tag="IT904_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAP3191658.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            16518..17702
                     /locus_tag="IT904_001872"
     CDS             16518..17702
                     /locus_tag="IT904_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191659.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            17826..18563
                     /locus_tag="IT904_001873"
     CDS             17826..18563
                     /locus_tag="IT904_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP3191660.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            18553..18888
                     /gene="ygaH"
                     /locus_tag="IT904_001874"
     CDS             18553..18888
                     /gene="ygaH"
                     /locus_tag="IT904_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAP3191661.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(18907..19008)
                     /locus_tag="IT904_001875"
     CDS             complement(18907..19008)
                     /locus_tag="IT904_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191662.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            18979..19509
                     /gene="emrR"
                     /locus_tag="IT904_001876"
     CDS             18979..19509
                     /gene="emrR"
                     /locus_tag="IT904_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAP3191663.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENNSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            19636..20808
                     /gene="emrA"
                     /locus_tag="IT904_001877"
     CDS             19636..20808
                     /gene="emrA"
                     /locus_tag="IT904_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAP3191664.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            20825..22363
                     /gene="emrB"
                     /locus_tag="IT904_001878"
     CDS             20825..22363
                     /gene="emrB"
                     /locus_tag="IT904_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAP3191665.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(22427..22942)
                     /gene="luxS"
                     /locus_tag="IT904_001879"
     CDS             complement(22427..22942)
                     /gene="luxS"
                     /locus_tag="IT904_001879"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP3191666.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(23092..24648)
                     /gene="gshA"
                     /locus_tag="IT904_001880"
     CDS             complement(23092..24648)
                     /gene="gshA"
                     /locus_tag="IT904_001880"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP3191667.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(24721..25149)
                     /locus_tag="IT904_001881"
     CDS             complement(24721..25149)
                     /locus_tag="IT904_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP3191668.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(25146..25712)
                     /gene="yqaB"
                     /locus_tag="IT904_001882"
     CDS             complement(25146..25712)
                     /gene="yqaB"
                     /locus_tag="IT904_001882"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAP3191669.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         6136 a         6634 c         6700 g         6507 t
ORIGIN      
        1 accatggtgc tgaagcctgc cagtcagacg ccgttctctg cgctggcgct ggcggagctg
       61 gcgattcgcg cgggcattcc ggctggggta tttaacgtgg tcaccggttc ggcgggcgcg
      121 gtgggtaacg aactgaccag caacccgctg gtgcgcaagc tgtcgtttac cggttcgacc
      181 gaaattggcc gccagttaat ggaacagtgc gcgaaagaca tcaaaaaagt gtcgctggag
      241 ctgggcggca acgcgccgtt tatcgtcttt gacgatgccg acctcgacaa agccgtggaa
      301 ggtgcgctgt cctcgaaatt ccgcaacgcc gggcaaacct gcgtctgcgc caaccgttta
      361 tacgtgcagg acggcgtgta tgaccgcttt gccgaaaaat tgcagcaggc ggtgagcaaa
      421 ttgcacatcg gcgacgggct ggagaaaggc gtcaccatcg ggccgctgat cgatgaaaaa
      481 gcggtagcaa aagtggaaga gcatattgcc gatgcgctgg agaaaggtgc gcgtgtggtt
      541 tgcggcggta aagcggatga acgcggcggc aacttcttcc agccgaccat tctggtggac
      601 gttccagcca acgccaaagt gtcgaaagaa gagacgttcg gccccctcgc cccactgttc
      661 cgttttaaag atgaagccga tgtgattgcg caagccaatg acaccgagtt tggccttgcc
      721 gcctatttct acgcccgtga tttaagccgc gtcttccgcg tgggcgaagc gctggagtac
      781 ggcatcgtcg gcatcaatac cggcatcatt tccaatgaag tggccccgtt cggcggcatt
      841 aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca tcgaagatta cttagaaatc
      901 aaatatatgt gcatcggtct ttaactggag aacgcgaatg agcagcaata aagagttaat
      961 gcagcgccgc agtcaggcga ttccccgtgg tgttgggcaa attcacccga ttttcgctga
     1021 ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt gagtatcttg atttcgcggg
     1081 cgggattgcg gtgctcaata ccgggcactt gcatccgaaa gtggtggctg cggtggaagc
     1141 gcagttgaaa aaactgtcgc acacctgctt ccaggtgctg gcctacgagc cgtatctgga
     1201 gctgtgcgag attatgaatc agaaggtgcc aggcaatttt gccaagaaaa cgctgctggt
     1261 caccacaggt tctgaagcgg tggaaaacgc ggtgaaaatc gcccgcgccg ccaccaaacg
     1321 tagcggcacc atcgctttta gcggcgcgta tcacgggcgc acgcattaca cgctggcgct
     1381 gaccggcaag gtgaatccgt actctgcggg catggggctg atgccgggtc atgtttatcg
     1441 cgcgctttat ccttgcccgc tgcacggcat aagcgaggat gacgctatcg ccagcatcca
     1501 ccggatcttc aaaaatgatg ccgcgccgga agatatcgcc gccatcgtga ttgagccggt
     1561 tcagggcgaa ggcggtttct acgcctcgtc gccagccttt atgcagcgtt tacgcgctct
     1621 gtgtgacgag cacgggatca tgctgattgc cgatgaagtg cagagcggcg cggggcgtac
     1681 cggcacgctg tttgcgatgg agcagatggg cgttgcgccg gatcttacca cctttgcgaa
     1741 atcgatcgcg ggcggcttcc cgctggcggg cgtcaccggg cgcgcggaag taatggatgc
     1801 cgtcgctcca ggcggtctgg gcggcaccta tgcgggtaac ccgattgcct gcgtggctgc
     1861 gctggaagtg ttgaaggtgt ttgagcagga aaatctgctg caaaaagcca acgatctggg
     1921 gcagaagttg aaagacggat tgctggcgat agccgaaaaa cacccggaga tcggcgacgt
     1981 acgcgggctg ggggcgatga tcgccattga gctgtttgaa gacggcgatc acaacaagcc
     2041 ggacgccaaa ctcaccgccg agatcgtggc tcgcgcccgc gataaaggcc tgattcttct
     2101 ctcctgcggc ccgtattaca acgtgctgcg catccttgta ccgctcacca ttgaagacgc
     2161 tcagatccgt cagggtctgg agatcatcag ccagtgtttt gatgaggcga agcagtagcg
     2221 ccgctcctat gccggaggcg acgctgcgcg tcttgtccgg cctacgggga tccaggtcgg
     2281 ataaggcgtt tacgccgcat ccgacaatct gtacgtgaac aggaagaaat ctatgttggc
     2341 cgggtaaggc ggagccgctc tccggcaaaa agaatcaata acaattatac gcgtgacccg
     2401 gcgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat tcaggatggg
     2461 gcaatcatcg caaccacatg agttaggcgg cgggctgaag tcacgccacg tcaccatgtt
     2521 gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga
     2581 agcgggcccg gcggtattac tggcctatct gttcgccggg ctactggtgg ttatgattat
     2641 gcggatgttg gcggaaatgg cggttgccac gcccgatacc ggttcgtttt ccacctatgc
     2701 cgataaagcc attggtcgct gggcgggcta taccatcggc tggttgtact ggtggttttg
     2761 ggtactggtt atcccgctgg aagccaacat cgccgccatg atcctgcact catgggttcc
     2821 aggcattccc atctggttat tttccctcgt cattaccctc gccttaactg gcagtaactt
     2881 attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca aagtcatcgc
     2941 tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc cgtatgccga
     3001 agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg gtttcggtgc
     3061 ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat
     3121 tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgccacca actcggttat
     3181 ctggcgtatt tctatcttct atttatgttc tatttttgtc gtcgtggcat taattccgtg
     3241 gaatatgccc ggactaaaag ccgtcggttc ttatcgctcg gtactggaat tgctcaatat
     3301 tccccatgcg aaattaatca tggactgcgt gatattactt tccgtaacca gttgcctgaa
     3361 ctcggcgctg tataccgcgt caaggatgct ctactcctta agtcgtcgcg gtgatgctcc
     3421 cgcggtaatg ggtaaaatca accgcagtaa aaccccgtac gtggcggtgt tactctccac
     3481 cggcgcggca ttcttaacgg tggtggtgaa ctattacgcg cctgcgaagg tatttaaatt
     3541 tctgatcgac agctccggcg ctatcgccct gctggtttat ttagtcatcg ccgtttcaca
     3601 gttgcggatg cgcaaaattc tgcgagcaga aggaagcgaa attcgcttgc gaatgtggct
     3661 ttatccgtgg ctcacctggc tagtcatcgg ctttattacc tttgtgttgg tagtgatgct
     3721 attccgtccg gcgcaacagt tagaagtgat ctccaccggc ttattagcga taggaattat
     3781 ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta ttgtggcaaa aaacacccgt
     3841 tcataatacg cgctgatcat gatcaggagt cacaccatga ccattacgtc tctggatggc
     3901 tatcgctggc tgaagaacga tattattcgc ggtaattttc aaccggatga aaaattacga
     3961 atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc cgttacggga agctctttcg
     4021 caactggtgg cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct
     4081 atgtcacagc aggagctgct cgatattttc gacgcccgcg ccaatatgga agcgatgctg
     4141 gtcagtctgg cgattgcccg cggtggcgat gaatgggagg cagacgttct cgcaaaagcg
     4201 catctgctga gtaagctgga ggcctgtgac gccagcgaga aaatgcttga tgagtgggat
     4261 ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg
     4321 caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc gatttatctg gctgcggcga
     4381 acggtgcttt cggtagaaat gctggaggat aaacacgatc agcaccagac cctgactgcg
     4441 gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca tttactgacg
     4501 ccaattccca ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa
     4561 cacttgcccc ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat
     4621 tttattgtac agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc
     4681 agacttaacg gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc
     4741 aacactggca ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg
     4801 actcaggccg tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc
     4861 ggcatcaggt ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt
     4921 atcgtgctga cctgtaaccg cgtcccagag tttttctccg gcatctttca caaaattgaa
     4981 cagacccata gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc
     5041 ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg
     5101 tgaatcaaac cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa
     5161 ccgaaccctt taccgagcag cacgccgagc ggcggcagaa tgatggtgat gacgattctc
     5221 cagaaaccca tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc
     5281 tcttaagcgt agtaatttta gcggagcctg ccagtttcag cttacgcttt tactctcatt
     5341 tacgctgctg aggctggtat atgttgcatt tttagtttag agtttactta atttataaaa
     5401 tacttaaata aatatgactg aactcgcgca attacaggcc agtgccgaac aggcagcggc
     5461 cttattgaaa gcaatgagcc atcctaaacg attgctgatt ctgtgcatgc ttagcggttc
     5521 ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagtgcct ctgcgacatc
     5581 acagcatctc gctcgtatgc gggacgaagg gcttatcgac agccaacggg atgcccaacg
     5641 cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt
     5701 ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga
     5761 attaatcgca cgcggcgcaa agttaatcga tattcgtgat gctgatgaat atcttcgtga
     5821 acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccggc
     5881 taaattacgt cacgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa
     5941 tgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat
     6001 tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt
     6061 aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta
     6121 taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc
     6181 aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg
     6241 agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagagc
     6301 aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg
     6361 ccctgaccgg tccaggtttt agtttcaccg ttaacatcgg tgaatttata tttcgccgga
     6421 cgcggctggc gttttttacc agcgcgtggt gcagcagcag agctattacc taataactct
     6481 tccgggttaa ttccgtcagc cttcatcagc tccagccaag tgctaatttt ttcctgacgc
     6541 tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg
     6601 aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggaga
     6661 gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc
     6721 caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa
     6781 tttaatctat ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctcag
     6841 aataatttca agattatata ttagccattt tgatgagtaa tttagattat tggtgaacag
     6901 aacggcgctc tctgctcgct aattccgcaa gcctgacaaa accgaagtga ataaaaatgc
     6961 aatataacag aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt
     7021 tctggcataa aaaatactgt aaatgacacg gtcacgacat acaccgaatt ctctacgctt
     7081 atagcgttac ctcaccccca aactttcatt agtgaaatta aaggaaaggt catatctaat
     7141 tgatttaatt gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaacgag
     7201 ccgttacgga atattttatc tacaaaaact gactaaataa aaatttttca ctaattgatt
     7261 agtcatagcc agcgatatac gctatgcgaa aatgcagatg gcaatgagat ccactgtttt
     7321 catctccatt aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag
     7381 tcacgcttgc gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg
     7441 aacatgtgta ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga
     7501 ttggtagcga ttccggtgaa catcttaatt gcatggccat acggtatgta ccgtgatctg
     7561 tttatgcgcg cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggcggatatc
     7621 ctggcttatg tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca
     7681 gactggcatc agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg
     7741 gcggtttatg gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag
     7801 caggtaaaag cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc
     7861 ttcgccaaat aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg
     7921 gataccgtaa tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc
     7981 gcaagtttta atatcggaag cgggttcagc cagtgtcgaa ctgcgttctt ttaatgttgg
     8041 atgaattacc agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc
     8101 cccacggcgg taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc
     8161 aagtacgcgc gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attatcaacc
     8221 gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc
     8281 gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg
     8341 ggatcatatg tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca
     8401 gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga
     8461 cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcattgctga aagaaaccag
     8521 ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgcgc
     8581 ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat
     8641 ttttatcggc gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata ataaatacgc
     8701 gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc
     8761 ccctaccccg tcacgctcat atccagggta atttcgacca ctatttgcta tatattgtgt
     8821 ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg
     8881 cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc attactattt
     8941 acactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct
     9001 ttgattttga aatgattaat gtcgatcgcg ttcctgaagc ggcagaagcg ttgcgtgctc
     9061 agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc
     9121 cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt
     9181 ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc
     9241 ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat
     9301 cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt
     9361 tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa
     9421 ctttggtgag gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg
     9481 gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt
     9541 aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac
     9601 gcgctgaatg cgatgcttaa cctctacgat agcgcaggtc gcattcagtt cgataaagac
     9661 cgccaggccg ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt
     9721 agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt
     9781 aatcgctact ctcgcgactt tgtcattacg ctgtttgccc acgcaaacac cagcggtttt
     9841 cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc
     9901 gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg
     9961 gcacaaggcg atgagacgct ggcgttgcaa ctgacagatg aaatgctgtc aggacgcttt
    10021 cagccagcca cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc
    10081 tgttttttgc tgcgtattga agacaatatg gagtcgattg gtcgggcggt aaattccgca
    10141 ctgcaactgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgagaagcg
    10201 ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg
    10261 ctggaagacg cattttccta tgccaaccaa ctcggcgctc gtcagggggc tggtgcagtc
    10321 tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc
    10381 gacgaaaaaa tccgcattaa aacactgtcg cttggcgtgg tgatcccgga tatcactttc
    10441 catctggcaa aagagaatgc gcagatggcg ctgttttcgc cttatgacgt agagcgagtt
    10501 tatggcaagc cgtttgccga tgtggccatc agccaacact atgacgaact ggttgccgat
    10561 gaacgcattc gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc
    10621 cagtttgagt ccggctatcc ctacatcatg tatgaagaca cggtaaaccg tgctaaccct
    10681 atcgccgggc gcataaatat gagtaatctc tgctcagaaa ttttgcaggt taacagcgcc
    10741 tcagagtatg acgagaatct cgactatacc cgcacaggcc atgatatttc ctgcaattta
    10801 ggttcgttga atattgcgca caccatggat tcccccgatt ttgcccgcac ggtagagact
    10861 gccgtgcgcg gtttaacggc agtatcagat atgagtcata tccgcagcgt gccgtccatc
    10921 gaagccggaa atgccgcctc gcacgccatc ggactggggc agatgaattt acacggctat
    10981 ctggcgcgag aaggcatcgc ttatggttcg ccggaagcac tggatttcac caatctctat
    11041 ttctatgcca tcacctggca tgcactgcgt acctcgatgt tgctggcacg cgaacgcggt
    11101 gaaaccttcg ccgggttcaa acagtcacgc tatgccagtg gtgaatattt tagccaatat
    11161 ctgcaaggga actggcagcc gaaaacggcg aaagttggcg aactgtttac ccgtagcggt
    11221 attacgttac ctacccgtga gatgtgggcg cagctgcgcg acgacgtgat gcgctacggc
    11281 atatacaacc agaatcttca ggcggtgccg ccaaccggtt ctatctctta taacaaccat
    11341 gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca
    11401 ggacgcgttt actaccctgc cccgtttatg actaacgaga atctggcgct gtatcaggac
    11461 gcttacgaaa ttggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc
    11521 gatcaggggc tgtcgctgac gctttttttc cccgataccg ccaccactcg cgatatcaac
    11581 aaagcgcaga tttacgcctg gcgcaagggt atcaaaacgc tctattacat ccgcctgcgt
    11641 cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata
    11701 tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat
    11761 ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg
    11821 aacgatattc ctgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt
    11881 tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttctctgatg
    11941 cccgatgcac tcacgcctca tgaagaagcg gtattatcga atatcagctt tatggaagcg
    12001 gttcatgccc gctcttacag ttcgattttc tcgacgctat gccagaccaa agatgtcgat
    12061 gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag
    12121 caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct
    12181 tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc
    12241 aataccgcgg acctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata
    12301 ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag
    12361 agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag
    12421 ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacgccaat
    12481 aaggctttga tgaatctggg ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat
    12541 ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt
    12601 tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc
    12661 tgagggtgtt attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata
    12721 gctttttatc acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc
    12781 ctgaaactac aatattcagg cgttttttcg ctatctatga caaaaaatat caacttttct
    12841 cgatttgctc tcagccctta tatcacggga aattccggcg atttgctcgc atcaatattc
    12901 atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt
    12961 gactatttcc attgggtaat atatcgacat aaacaaataa aggaatcttt ctattgcatg
    13021 gcaattaaat tagaaattaa aaatctttat aaaatatttg gtgagcatcc acagcgagcg
    13081 ttcaaatata tcgaacaagg actttcgaaa gaacaaattc tggaaaaaac tgggctatcg
    13141 cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga
    13201 ttatccggct cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc
    13261 cgcgggcaag tactgattga tggtgtggat attgccaaaa tatccgacgc cgaactccgt
    13321 gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg
    13381 accgtgctgg acaatactgc gtttggtatg gaattggccg gaattaatgc cgaagaacgc
    13441 cgggaaaaag ccattgatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac
    13501 ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt
    13561 aatccggata ttttattaat ggatgaagcc ttctcggcgc tcgatccatt aattcgcacc
    13621 gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt
    13681 tcccacgatc tcgatgaagc catgcgtatt ggcgaccgaa ttgccattat gcaaaatggt
    13741 gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc
    13801 cgtaccttct tccgtggcgt tgatattagt caggtattca gtgcgaaaga tattgcccgc
    13861 cggacaccga atggcttaat tcgtaaaacc cctggcttcg gcccacgttc ggcactgaaa
    13921 ttattgcagg atgaagatcg cgaatatggc tacgttatcg aacgcggtaa taagtttgtc
    13981 ggcgcagtct ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg tcttgatgcg
    14041 gcgctgattg atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga gttgctctct
    14101 catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc
    14161 atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga
    14221 tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcgcaat ccgcagacgc
    14281 ctggggtaca ccgacgactg caccgactga cggcggtggt gctgactggc tgaccagtac
    14341 gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc
    14401 gctcgacagt tgggtcactg aagggatcga ctgggtcgtt acccatttcc gtcccgtctt
    14461 ccagggcgtg cgcgttccgg ttgattatat cctcaacggt ttccagcaat tgctgctggg
    14521 tatgcccgca ccggtggcga ttatcgtttt cgctctcatc gcctggcaga tttccggggt
    14581 cggaatgggt gtggcgacgc tggtttcgct gattgccatc ggcgcaatcg gtgcctggtc
    14641 gcaggcaatg gtgactctgg cgctggtgtt aaccgccctg ctgttctgta tcgtcatcgg
    14701 tttgccgttg gggatatggc tggcgagaag tccgcgagcg gcgaaaatta ttcgtccact
    14761 gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtgccaatcg tcatgctatt
    14821 tggtatcggt aacgtgccgg gcgtggtggt gacgatcatc tttgctctgc cgccgattat
    14881 ccgtctgacc attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc
    14941 attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac
    15001 cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg tcatcgcctc
    15061 gatgattgcc gtcggcgggt tgggtcagat ggtacttcgc ggtatcggtc gtctggatat
    15121 ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgatcgtct
    15181 gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg
    15241 ccctgtcggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc cggtgacgcc
    15301 gggcatatca ccctgccaaa aaaaggaata acaatgcgac atagcgtact ttttgcgaca
    15361 gcgtttgcca cgcttatctc tacacaaact tttgctgccg atctgccggg caaaggtatt
    15421 actgttaatc cagttcaaag caccatcacc gaagaaacct tccagacgct gctggtcagc
    15481 cgtgcgctgg agaaattagg ctataccgtc aacaaaccca gcgaagtaga ttacaacgtt
    15541 ggctacacct cgcttgcttc cggcgatgca accttcaccg ccgtgaactg gacgccactg
    15601 catgacaaca tgtacgaagc tgccggtggc gataagaaat tttatcgtga aggggtattt
    15661 gttaacggcg cggcacaggg ttacctgatc gataagaaaa ccgccgacca gtacaaaatc
    15721 accaacatcg cacaactgaa agatccgaag atcgccaaac tgttcgatac caacggcgac
    15781 ggaaaagcgg atttaaccgg ttgtaaccct ggctggggct gcgaaggtgc gatcaaccac
    15841 cagcttgccg cgtatgaact gacccatacc gtgacgcata atcaggggaa ctacgcggcg
    15901 atgatggccg acaccatcag tcgctacaaa gagggcaaac cggtgtttta ctacacctgg
    15961 acgccgtact gggtgagtaa cgaactgaag ccgggcaaag atgtcgtctg gttgcaggtg
    16021 ccgttctccg cactgccggg cgataaaaac gccgatacca aactgccgaa tggtgcgaat
    16081 tatggcttcc cggtcagcac catgcatatc gttgccaaca aagcctgggc cgagaaaaac
    16141 ccggcagcag cgaaactgtt tgccattatg cagttgccag tggcagatat taacgcccag
    16201 aacgccatta tgcatgacgg caaagcctca gaaggcgata ttcagggcca tgttgatggc
    16261 tggatcaaag cccaccagca gcagttcgat ggctgggtga atgaggcgct ggcagcgcag
    16321 aagtaatttt tattcgggcg gataaggcgt ttttacctca tccgccgctc tgtacacaat
    16381 gcctgatgcg acgctgacgc gtcctatcat gcctacaaac cacatcgcac atttcaacaa
    16441 gctatctttc atcccatatt catcaacatc cgctattatt gatttccagc ttaatcatca
    16501 cctgatgaac aaaaataatg actaaaccta atcatgagct tagcccggcg ctgatcgtgc
    16561 tgatgtctat cgccaccggt ctggcggtcg ccagtaacta ttacgctcag ccattgctcg
    16621 acaccatcgc gcgtaacttt tccctttccg ccagttcggc aggctttatt gttaccgccg
    16681 cgcaattggg ctatgcagca ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac
    16741 gccgccgcct gattgtctcg atgaccttac tggcggcagg cggcatgtta atcactgcca
    16801 gcagtcagtc gctggcgatg atgatcctcg gtacggcatt aaccggttta ttctctgtcg
    16861 tggcacaaat tctggttccg ctggcagcga cgctggcttc accggacaaa cgcggcaaag
    16921 tggttggcac tattatgagc ggtctgctgt tggggatctt gctggcacgg acagttgccg
    16981 gattgctggc gaatctcggc ggctggcgca ccgtcttttg ggttgcttcg gtgttaatgg
    17041 cactgatggc gctggcatta tggcgtggtc tgccacaaat gaaatcagaa acccacctca
    17101 actacccaca gttgttgggt tccgttttca gtatgtttat cagcgataaa attctgcgta
    17161 cccgcgcgtt gctgggctgc ctgacctttg ccaatttcag cattctctgg acctcaatgg
    17221 cctttttgct tgccgctcca ccttttaact acagcgatgg tgtcattggt ctgtttggac
    17281 ttgcgggagc tgccggagcg ttgggcgctc gtccggcggg cggttttgcc gataagggca
    17341 aatcgcacca caccacaact ttcggtctgc tgctgctatt actttcatgg ctggcgatct
    17401 ggtttggtca cacttccgta ctggcgttga ttatcggaat cctggtgctg gatctcaccg
    17461 tgcagggcgt gcatatcact aaccagacgg taatttatcg gatacatcct gatgcgcgca
    17521 atcgcctgac cgcaggttac atgactagct actttattgg cggtgccgcc ggttcgctaa
    17581 tttcagcctc agcctggcaa catggcggtt gggctggcgt ttgtctggct ggcgcgacaa
    17641 ttgccctggt taacttactg gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt
    17701 aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga agcccatcag
    17761 tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg ttaagcgtac
    17821 actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc atggaaggat
    17881 gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg ttcggtctga
    17941 atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc atcatttatg
    18001 caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt ttgtgggttg
    18061 ctgcactgac cgtcatggca atggatgttc gccatgtgtt gtatggcccg tcactgcgta
    18121 gccgtattat tcagcgtctg caaaaatcga aaaccgccct gtgggcattt ggcctgacgg
    18181 atgaggtttt tgccgccgca accgcaaaac tggtacgcaa taatcgccgc tggagcgaga
    18241 actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt acggtaatag
    18301 gggcattctc cggcagcggc ttgctgcaag gttatcccgc cgttgaagct gcattaggtt
    18361 ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc aaacaatctc
    18421 tttgcgttac cgcagcgtta gttggtgccc ttgcaggcgt aacgctattt tctattcccg
    18481 tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag gcattctggc
    18541 aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg cgtggcgaat
    18601 tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt
    18661 ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt
    18721 gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac gctggtcggc
    18781 ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc aacactgctt
    18841 agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc cacaagaatc
    18901 atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat cggctgaaat
    18961 taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat gctaaaattt
    19021 cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgactcg tctttgcatg
    19081 cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca ggggattaac
    19141 gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaacaacag tattcagcct
    19201 tctgaattaa gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa
    19261 ctggaaaaac gcggttggat cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat
    19321 ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc gcagcataac
    19381 tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagatcagct cgagcaaatc
    19441 acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt tctcgaagcg
    19501 atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa atgaccggcc
    19561 agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg agttaagaag
    19621 atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca accggtaaag
    19681 aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat aattattgcc
    19741 gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca
    19801 tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc
    19861 tgggccgata acaccgattt tgtaaaagaa ggcgatgttc tggtcactct cgacccgaca
    19921 gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt tcgccaaacc
    19981 caccagctga tgattaacag caagcagttg caggcgaata ttgaggtgca gaaaatcgcc
    20041 ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt
    20101 ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca gtgcccaggc gcaactggac
    20161 gtcgcgattc aacaatacaa tgccaatcag gcgatgattc tggggactaa actggaagat
    20221 cagccagccg tgcaacaggc tgccaccgaa gtacgtaacg cctggctggc gctggagcgt
    20281 actcgtattg tcagtccgat gaccggttat gtctcccgcc gcgcggtaca gcctggggcg
    20341 caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat gtgggtggat
    20401 gccaacttta aagagacgca gattgccaat atgcgtatcg gtcagccggt cactatcacc
    20461 acggatattt acggcgatga tgtgaaatac accggtaaag tggttggtct ggatatgggc
    20521 acaggtagcg cgttctcact gcttccagcg caaaatgcga ccggtaactg gatcaaagtc
    20581 gttcagcgtc tgcctgtgcg tatcgaactg gaccagaaac agctggagca atatccgctg
    20641 cgtatcggtt tgtccacgct ggtgagcgtc aataccacta accgtgacgg tcaggtactg
    20701 gcaaataaag tacgttccac tccggtagcg gtaagcaccg cgcgtgaaat cagcctggca
    20761 cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc cagaggtgcg
    20821 tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga cgattgcgct
    20881 gtcactggcg acattcatgc aggtgctgga ctccaccatt gctaacgtgg cgatccccac
    20941 tatcgccggg aatctgggct catcgctcag ccagggaacg tgggtaatca cttctttcgg
    21001 ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg tcggggaagt
    21061 gaaactgttc ctttggtcca ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc
    21121 cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg ccgggccgtt
    21181 gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac gctcgatcgc
    21241 gctggcgttg tggtcgatga cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg
    21301 ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc cgattggcgt
    21361 ggcggtggtg ttgatgacac tgcaaactct gcgcggacgt gaaacccgca ccgaacggcg
    21421 gcggattgat gccgtggggc tggcactgct ggttattggt atcggcagcc tgcagattat
    21481 gctcgaccgc ggtaaagagc tggactggtt ttcatcacag gaaattatca tccttaccgt
    21541 ggtggcggtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg ataacccgat
    21601 agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct
    21661 cgcgtatatg ctctacttcg gcgctattgt tctgctgccg cagttgttgc aggaggtcta
    21721 cggttacacg gcgacctggg caggtttggc ctctgcgccg gtagggatta ttccggtgat
    21781 cctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc tggtaacctt
    21841 cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg aaccaggtat
    21901 ggattttggc gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg cctgcttctt
    21961 tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg cggcggcatc
    22021 gagcctctct aactttacgc gaacgctggc ggggtctatc ggcacgtcga taaccacgac
    22081 catgtggacc aaccgcgagt cgatgcacca tgcgcagttg actgagtcgg taaacccgtt
    22141 caacccgaat gcccaggcga tgtacagtca actggaaggg cttgggatga cgcaacagca
    22201 ggcatcaggc tggattgccc agcagatcac caatcagggg ctgattattt ccgccaatga
    22261 gatcttctgg atgtcagccg ggatattcct cgtcctgctg gggctggtgt ggtttgctaa
    22321 accgccattt ggcgcaggtg gcggcggagg cggtgcgcac taagtacaac taagccagtt
    22381 catttgaact ggcttttttc aattaattgt gaagatagtt tactgactag atgtgcagtt
    22441 cctgcaactt ctctttcggc agtgccagtt cttcgttgct gttgatgcgt acgtcacgtt
    22501 ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg taagtgccac
    22561 actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc acgtcttcca
    22621 ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc agactcatat
    22681 aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca ttaccgttaa
    22741 gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct ctttctggca
    22801 tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg
    22861 gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta tgatcgactg
    22921 tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa attttctgaa
    22981 ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat catcatccct
    23041 gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttct gtcaggcgtg
    23101 tttttccagc cacaccgcaa acggttcggt atcagcggct tccatttcct gctgacggcg
    23161 ttcagacgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc
    23221 acgcagcaga ttacggtagg cttctgcaaa tgctttgcct gttccgccaa taccagtatc
    23281 aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga agcaggcaac
    23341 cagttcatca cacactttct gatacgcttc gccgccgtta atactatcca gcgtttgcgc
    23401 gacgcgtttc agatcgcgga acagatcttt acccacctgc ggtaacggga actgtgcggt
    23461 ttcgcagccg atacccagcg tcagacccgg tttgcgacct tcgaggatca cccggttcca
    23521 gttaacgcgt gtacaggcaa gttcgctact gctcatttcc ggtgcatcag ccagcgcaca
    23581 ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa
    23641 cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta acagcgcatc
    23701 agaaggcgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcgt acagttcgtt
    23761 ttcaatctgc aacacgttgc tgttgatttg cagcctctta ccgtctttct caataccaat
    23821 cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta cgtactcgta
    23881 aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc cgagatcgct
    23941 caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt tctcaaacgg
    24001 cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc gccggagatg caccaaacag
    24061 ataaggaatg acccaaccga aacgatagta attgcggata acgcggaaat agcccgcaga
    24121 aattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc agaatgccat
    24181 tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt
    24241 tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc
    24301 cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc acatccgctc
    24361 atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca gcatatgttc
    24421 aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat cggtagtaat
    24481 ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc
    24541 atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg
    24601 aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg
    24661 cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc aggcctacaa
    24721 ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat agcgtaacgc
    24781 tttaccaagg cacaaaaaaa agattaccgg tccccacgag atgcgcatcc atcccgctaa
    24841 cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga ctgcaccata
    24901 gcgtttcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca atggaaagaa
    24961 acgtccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg ctgttaaaac
    25021 taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga cttcggagtt
    25081 gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa gcgataacgc
    25141 ttcactcaca gcaagcgaac atccacggcg tccatgcctg ctgcacgggc cgcctgaata
    25201 ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg
    25261 cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcggc agcgacgacg
    25321 gcgtcaaaat aatggcgtaa tcccaggtgc gccagcaatg cctcagcgat ggcgctttca
    25381 ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac atcaacaaga
    25441 ggaagcggtt cgacgctatc cagcagcata cttcttactg cttctgtttt ttcacgcgct
    25501 aacgcatgcg ggtcgagatc ggcctgattc agctcaataa ttgcctgagc aatacgccag
    25561 gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt actgaagacc gtagtgccct
    25621 aatacttcgc gccacgcttt acggtgcgta ggctccgtat ccaggattgt gccatccata
    25681 tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga aaaatatgac
    25741 gctactttag cgtaattgct tgattttgtc gctgatggcg aaggagaaga aaattcagga
    25801 aatggataaa ggagttattt agaaatgaga tatttttgaa ggaaattttt tggagaagat
    25861 ggtgcatccg ggaggatgac tcactgacgt tcgcccttcg ggccgttgct ggcgcaacgt
    25921 tatccttcct ggtgcctgcg gttaataccg ctatctcttc gataccttca ttgctga
//
LOCUS       DACAPB010000051        24733 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.051, whole genome shotgun sequence.
ACCESSION   DACAPB010000051 DACAPB010000000
VERSION     DACAPB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.051"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(97..3897)
                     /locus_tag="IT904_001883"
     CDS             complement(97..3897)
                     /locus_tag="IT904_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAP3191670.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNNKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLASTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFDLTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNLGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(3965..5434)
                     /gene="rng"
                     /locus_tag="IT904_001884"
     CDS             complement(3965..5434)
                     /gene="rng"
                     /locus_tag="IT904_001884"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAP3191671.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(5424..6017)
                     /gene="yhdE"
                     /locus_tag="IT904_001885"
     CDS             complement(5424..6017)
                     /gene="yhdE"
                     /locus_tag="IT904_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAP3191672.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASDEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(6026..6514)
                     /gene="mreD"
                     /locus_tag="IT904_001886"
     CDS             complement(6026..6514)
                     /gene="mreD"
                     /locus_tag="IT904_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP3191673.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIFFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(6514..7617)
                     /gene="mreC"
                     /locus_tag="IT904_001887"
     CDS             complement(6514..7617)
                     /gene="mreC"
                     /locus_tag="IT904_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP3191674.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(7683..8726)
                     /locus_tag="IT904_001888"
     CDS             complement(7683..8726)
                     /locus_tag="IT904_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAP3191675.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(9031..10971)
                     /gene="csrD"
                     /locus_tag="IT904_001889"
     CDS             complement(9031..10971)
                     /gene="csrD"
                     /locus_tag="IT904_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAP3191676.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQITVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11123..12097
                     /locus_tag="IT904_001890"
     CDS             11123..12097
                     /locus_tag="IT904_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP3191677.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            12134..12964
                     /locus_tag="IT904_001891"
     CDS             12134..12964
                     /locus_tag="IT904_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191678.1"
                     /translation="MKSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREK
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            13830..14300
                     /gene="accB"
                     /locus_tag="IT904_001892"
                     /gene_synonym="fabE"
     CDS             13830..14300
                     /gene="accB"
                     /locus_tag="IT904_001892"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP3191679.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            14311..15660
                     /gene="accC"
                     /locus_tag="IT904_001893"
     CDS             14311..15660
                     /gene="accC"
                     /locus_tag="IT904_001893"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP3191680.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            15769..16011
                     /locus_tag="IT904_001894"
     CDS             15769..16011
                     /locus_tag="IT904_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAP3191681.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLSGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            16001..17452
                     /gene="panF"
                     /locus_tag="IT904_001895"
     CDS             16001..17452
                     /gene="panF"
                     /locus_tag="IT904_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAP3191682.1"
                     /translation="MQLEVILPLVAYLMVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            17464..18345
                     /gene="prmA"
                     /locus_tag="IT904_001896"
     CDS             17464..18345
                     /gene="prmA"
                     /locus_tag="IT904_001896"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP3191683.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            18674..19639
                     /gene="dusB"
                     /locus_tag="IT904_001897"
     CDS             18674..19639
                     /gene="dusB"
                     /locus_tag="IT904_001897"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP3191684.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            19665..19961
                     /gene="fis"
                     /locus_tag="IT904_001898"
     CDS             19665..19961
                     /gene="fis"
                     /locus_tag="IT904_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAP3191685.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(20039..22327)
                     /locus_tag="IT904_001899"
     CDS             complement(20039..22327)
                     /locus_tag="IT904_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP3191686.1"
                     /translation="MANKKLNATITIGGAVSGSLRGAFGTVEKSTIKIGAAIKHMSRE
                     QRQLNDAMKRYAQDGAMVGRMKERYQAVVGQVERLRAAQERLNRVQRASAENLAKQAQ
                     LRGQIIDTVMAGAAVAAPIKIAADREQHAIGIAKQLDGARDAAGNLTAKFWEMRKAVA
                     DLGHEIPLATNDLFDMATAGLRMGVAGDQILGFTRNVAKLASALELNPEEVADNMGKI
                     QNVYRLTQAELVRLGDAINYLDDQNTVKGGELIDFLQRVGGSASLAKLTANDMAAIGT
                     TLISMGESADTAATSVKALVSKMTLGSKSTKSAREAFKELGYSAQEVAKSMQLDSVKT
                     IENFMKTVNKLPDYKKSGILVSIFGQEHVGSISKLAANMDKFGEALHQANGEMSKGSV
                     QKEFQNTVNTTNAQMVILRNRTADVADNIGTVLLPTVNDAAKGIGRITTVVANFAAEH
                     PGLTKAVVGTAMALTSLRVATLAAQFGFTFLKGGVLQMSSLFARMNASAVLASTRGLP
                     AVATGIRAVGAAFISTGVGAAVAGLALAGYQIYRHWAGVKAFFGGVGEGIKSGLEPLS
                     DALSRLYERMGPLKPVIDGVGAAVKTVFNWFTSLTEPVKYSGEELDKAGKMGMTFGKT
                     LAAGIELVTAPITFLIDKILWVSDNIGNLTNKALEFKNAVSDVAGGAWQKTKDFFTAP
                     FRDDVQQPAATTASGAALPSPALANRSGGTTVHSNDQYHISVKAEPGMNEDALARKVI
                     QQLRQQQAVRQRSMMIDGAQTP"
     gene            complement(22349..22555)
                     /locus_tag="IT904_001900"
     CDS             complement(22349..22555)
                     /locus_tag="IT904_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191687.1"
                     /translation="MLDRIHTKPEGTNPWPELVDMVLPKVMVCETAEGMQAQRKHRTE
                     CALQLAAAALRAAGEHTLAENLLN"
     gene            complement(22565..22774)
                     /locus_tag="IT904_001901"
     CDS             complement(22565..22774)
                     /locus_tag="IT904_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191688.1"
                     /translation="MATNNNIYEAVLNVIGVADKMTKNNASQLTDAQKLALKNLSGAM
                     KSWKGITFGMRDITANDKPTESKTA"
     gene            complement(22777..23094)
                     /locus_tag="IT904_001902"
     CDS             complement(22777..23094)
                     /locus_tag="IT904_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191689.1"
                     /translation="MKNHDLYESMKEEARLILSETKRFQTISEKNPISYEAADLLASL
                     RNRLNAFEHMTDEAGRIMRDEAESHASTLERKKWQPSSRISSGERAKRQAVKRGAILV
                     RSA"
     gene            complement(23477..23791)
                     /locus_tag="IT904_001903"
     CDS             complement(23477..23791)
                     /locus_tag="IT904_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191690.1"
                     /translation="MWWGSKKAAAERAALTGEILAAIEGVAMYVRHGDENTPRIVVQP
                     VGWSGFIYSDDAAEKWIRKAYPELKQYQIERAVNYLASLVRSHYREQRPKQKRESWMN
                     RY"
     gene            complement(23794..24678)
                     /locus_tag="IT904_001904"
     CDS             complement(23794..24678)
                     /locus_tag="IT904_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191691.1"
                     /translation="MNVLSQLINDALNHQGGHIRTDANGEITQFIPVEPAAVRYLSMN
                     DVLSPKGGKQTTINAVLAERSLVAQAGAEIIRIPGPMTTQPTGKTGATTGREVADRFV
                     VIRPGKFAKVADGEEITMSGLPYLVSAFNHNNAPAYGVGYTLSRKQMKHDFADDTTLE
                     VVNTAIERGIADLSDFVLLNHLESVAENLTSPSFTAVAQKIAAKNLRFDEVKAIVGGD
                     CTGLELQDGVLRAYGVRAEISGQTSSTIIGAFGNAAVALDDEIRVTARRVLNGAVEVV
                     VWVNASALVPDSTMFWKA"
BASE COUNT         5730 a         6651 c         6361 g         5991 t
ORIGIN      
        1 gtgaacgccc tatccgacct acggttatgt tctggctcgt tggcctttgg caacgattat
       61 cctatgagat tggggcaatt acgcgccctc gtcaaatcat tgcgcttttt ctttacgcgg
      121 ttggcgcaac acttcgttga tttgcggatc gtccagcgga cccgaaatgt gatagcgcaa
      181 aatggagact ttgctccaca gcggccccag cactttactg gcggcaaaca ctgccgcgcc
      241 aacaatgggg ttaaccgcaa aagccgcagc cacgcccacc gtcgcagaaa tctctggtgc
      301 gacaaccgct tccatattca ggtcgcgacg taccagattt accgaccctt tcatggcgat
      361 atccgcctcc aggccatcca ccagcgtgtc gtcggtgtgc ataacgccgt ctttaatcca
      421 cgcggtgctg cgaatggagt caaaatagaa cccttcgcca aaagtgtctc tgaaatcaaa
      481 acgcagctta cgcatcaggg catctacgct caataagcgc agcaattgcc cggcatgtcc
      541 ggtattgatt tcggtaattt cgcctttacc cagttgagta tgaatgatgc cattcaacgt
      601 cgcctcatct ggctgccacg gtgctttacg ccagtgtaaa tcgtaatcca cattaaatga
      661 cgactgccgt atgggcgtcg tgacaccaaa aaattctgcg gcggcatcaa ttttctgccc
      721 gcgcagtttt cctttcagcg aggtacgttc attccccaga ttattaaccc attcaccatc
      781 ggcagtaagc cgcgagaaac cagtatcaat cagtccattg gtcagcgtta atgtatcgcc
      841 agaaatggtg agatcactgt caatacgacc gaatttttgc ccccagaacc agcactctgc
      901 gcatcgtatt tgtgcgtccg gccagccacg gaagttaatg cgctccgttg tcgggaaagg
      961 tgatgacggc gttgaatcac cacgagtttt cgccacgctc gggttgtaat aaagatattt
     1021 gatattcgcc agccacggcg cgttattacg catcgccagc gtggcgttga tttcacgccc
     1081 ttgcgcctca acctgggtgc catttgccgt cggttgcgaa acaatactca ggttattcca
     1141 ttgctgattt cccagcgaca acataggcgt acgtaacgtt atgtgttgtg ggaaactcgc
     1201 tgcaccaccg acactctccg ccgcaccttt ctgaaacagg gccagccact cggcaccatt
     1261 catcggcggc atattgagtt caacaccact ttgttccggc aacggcggga gcgttttact
     1321 gtctgccgcc caaatagcac ggtcgagcgt cagcttttga ccgagcaacc agcggctatt
     1381 gaaatggtta tccgcaccag cctgtccggt taaatcaaag ctgttgagat tgccatcaac
     1441 cttaacgttt accggcagtg gttcacccgc aggtttggct aacggtgaag gtaagtgact
     1501 gctcacattc ttcaaatcgc cgtttagctc tacgttatac gtcgcgccag catgataagg
     1561 cagaacaatg cccactttac catcccacgc cacgctgcca ctcaatgctt cgttcaccgc
     1621 tgcaggcaga acgccggttt tcgccggttg ccagttaccg ttgaggttta ccgctacctg
     1681 gtaggctttt gccccttctt tggtggaaaa atccacgttc aacggctgat taaaccagct
     1741 tgctgtcagt ggttcacttt gcagatcgcc attgataaag ctgaatttac cgctcaaatt
     1801 tttcagggtg ctggcgagtg gtttgataaa cagactgtta ttacgcagcg tcacttcacc
     1861 tttcgcggtt accagctcgc cgttcagcgg gatatcaaga tgtaagcgag cattcacatc
     1921 gccgtcgagc tggagttctt gcagggtcgc acccagagaa tctttcagcg gtgtctcatc
     1981 aaagtaaggg ccaacggctt tacccggacc tttaatgtca gcgtcaatca gcagtttttc
     2041 ttttgagtag tcagggatca ctgcggtaag attactcgcg cgcacgccgc ccagattaac
     2101 gccatcggtt ttcatccata aaccgtcgtt aataaagtcc agttcaatat caaggttagt
     2161 taatgcaggc cagtccggct ggaaggcaaa cttcgcgttg cgcagcggca ccagcacttc
     2221 aaactgacct tcgttgtgtt tataggggaa gagttgcgga ttgccaccat aaaccagcgt
     2281 cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag taatcaacca ggtctttacc
     2341 catcaagttt tccgggaaat agcgccaggc ttgtgaacca tcatcggtac tgatgccagc
     2401 cagaataccc agccagggtt catcgttagc aggttgcagg taacgaaaac cgccgcgcgc
     2461 atggacggct ttggctttaa cgtcaatatt acgcccatcc agctggaaac ctttgttatt
     2521 gttcagccag cttatagttg cctggccgtc ggcgatttct agtggcgcac ggaataccgt
     2581 ttcgtaaggc atctttgcct gcttcatcga cgcggtaagc aaaccatttt caacgctgcc
     2641 ggaaagcgtc ccggagaagt gttccgcacc cggtaataat ttccattgct tccaggccag
     2701 atcgctccac gatgcctgaa aacgggtctt gtctgccgcc tgaagcggga tatccagcgc
     2761 cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa acatcaccca gtgcaggtga
     2821 aagtttcgcg gccagcgggc gtatgccctc caggcctgcc agctccagat tactggcgcg
     2881 aatccggagt tcgtcactgc gtttattgtc tttgccgcca acgtcctgtt ccggtatcca
     2941 ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc atcgtgatcc gtgtatcggg
     3001 aatagagaac tgccagcccg gattttcacg cgtaatatgc gcggtcagat tatccaccga
     3061 cagcgtatgc gtttgcttct cgcccaacca gctggcaccg ccctgtttca gccagacgtc
     3121 accgccggtt acatcgcctt tgtcgatcgt catccagcct tcaagggaga actgtgccgt
     3181 ttccagcgca atattgtcct gcatccattt accgagccac ggcttcaggt cgatgtcatc
     3241 cgcctggagc cagacgcgac cattgcttaa caacccctca tcatcgcgca aatccatgcg
     3301 cacctgcatc acgccgtgct gtccggtaag gctggagagg cttaccaggc cttccgcacg
     3361 gtgtcgacgt ggatcgttca gccaggtgag ttgtgggatc gccagctcgg cgcgctgacc
     3421 ggatggcgtc aggaaactga cttcactgtc gcgaagatcg aaatggtcaa attgacgaag
     3481 aaacagatcg ctgatgtgac tggcttccag actgtcatca ctaccaccgc tggtgatagg
     3541 agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg cgaaactgcc agcgcatatg
     3601 taacaggctc tgccagacat ccagcgccag agtaacgcgt ttaaccgaaa attcgccgcc
     3661 atcttttagt tctgcacgga tgtcgtgtgc ttcaagcgtc gggccaaaat tctgccagct
     3721 ggctgagagc tgactggctt ctaccggcat gccagtcgcg gattctattt tgttgaggat
     3781 ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc aggccgctaa ccagcagggc
     3841 agcgatcaca acgagcgcgg ctccagtaag cagtaaaatc cccggcaatc gcctcacccg
     3901 tcactccttg tctgctaaaa atgtgactca aaaacccttt gccggatggc ggcccagcat
     3961 ctgtttacat cattacgacg tcaaactgct cctggttata gagcggttca atttgtactt
     4021 taacctgttt gccaacgaaa atttccactt ccgccagcga gtgtgactct tcgcctttca
     4081 aggcttcagc tactgccgga gaagcataga ccaggaaacg gtcggagtcg taagcatggt
     4141 ggacacgaac aatctcgcgc atgatttcat agcataccgt ttccacggtt ttcaccgttc
     4201 cgcgaccgtg gcaggttggg cattcgttac acagtacgtg ctcaatgctt tcgcgggtgc
     4261 gtttacgcgt catctccacc agccccagcg ccgaaaaacc attaacgctg gttttcaccc
     4321 ggtctttgct caacgcctgc tccagcgagt gcagcactcg gcggcggtga tcttcattat
     4381 tcatatcgat gaaatcaata atgataatcc cgcccagatt acgcaaccgt aactggcgag
     4441 cgatagcctg cgtcgcttca atattggtat tgaaaatggt gtcgtccaga ttgcgatgac
     4501 cgacaaacgc tccggtattg atgtccacgg tggtcatcgc ttcggtctgg tcgataatga
     4561 gataaccacc ggatttcagt tctactttgc gttccagcgc tcgctggatt tcgttttcga
     4621 catcaaagag atcgaaaatc ggctggcgtc ctgtgtaatg ctccagcttg cttgtcatct
     4681 cgggaatgta ctccgaggtg aactcaagta acgcttcgta agtcaggcgt gagtcaacgc
     4741 gaatgcggtc cagttcggca tcggcgaaat cacgcagaac acgctgcgcc agcgccagtt
     4801 cgccgtacag ctgataacgg gtctgcgggc gttttttacg ctccataact ttggtccaga
     4861 cgcgtttcag ataagcggca tcggaggcca gttcagcctc gccaacccct tccgctgcgg
     4921 tacggatgat aaacccgccc tgctcgtcgc aatactctgc gaccactttt ttcaggcgtt
     4981 cacgttctga ttcgctttca atacgttggg aaaccccaac gtgagaagcc cctggcataa
     5041 acaccagata gcgagaaggg agcgtgatat cggtggtcag gcgcgcacct ttagtgccaa
     5101 gcggatcttt caccacctgc accatcagat cttgcccctg acgaaccagt tccgagatgt
     5161 cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca ttcggtgtgc ggcatgatgt
     5221 cggatgcatg aagaaacgcg gctttatcca gcccaatatc tacaaaagcc gcctgcatac
     5281 ccggaagtac acgacttaca cgacccttgt agatattgcc tactattccg cgtcgcgcct
     5341 cacgttcaat atgaatttcc tgcagaatac cgccatcaat atacgccact cgcgtttccg
     5401 aaggcgttac gtttactaac aattcagccg tcatgtttat cccttttctc acgcagtgcg
     5461 ttaaaattac ttaataattc atacgtttca accagcggta agccgactac ggcgtgatag
     5521 ctgccattta tcttcctgac aaaacagcca cccagcccct gaataccgta tgcacctgct
     5581 ttatctaacg gttcatcgct ggcgacatag cccgcgatgt cttcgtctgt taacgttctg
     5641 aaagtcacat cggtgaccac caggcaatcg agaatgtgct ggctgtcggc caacgccact
     5701 gctgtcatca cctgatgggt ctgacccgat aatttgcgca acatctgcgc cgcatgctct
     5761 gcgtcgcgcg gtttctccag cacttctccg ttcaggataa cgatagtatc cgcacccagc
     5821 accgggagat ccttcgccgt ttgcgcgaca cctgcccgtg ctttctcgcg cgccagacgc
     5881 acaacatact gctgcgcgct ctcctgcggc tgacgctgct cctcaatgcc cgtaacaata
     5941 cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac gacgcggaga accggaagct
     6001 aaatacagag aagtcataga aacctttatt gcactgcaaa ctgctgacgg actttgcgca
     6061 tcagcaagaa aatccacggc cagagcaccc cattgactac actactccag aacacttccg
     6121 gtctgaaaga gacgttaatc actaaaaact ctgcccagaa aacaataata tccaccacca
     6181 gcgaaagcaa catgacgacc agcgcctgct gccataatgc gaggttgcgg aaaagctggt
     6241 atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa tacgcgtacg ccaagcgtcg
     6301 agccgctgat cagatccagt atggcaccca tcacaaaacc tgtgcccaca tttacgcgat
     6361 gaggcaaggc caggatccaa tacaacaaga tgagtaacac ccagtttggc cggaaaaaaa
     6421 tcaggttatc cggccagggc atgatttgca gcaacagcgc aatgaggaaa gagagccaga
     6481 ttacccagcg tccctggcta cgatagctcg ccactattgc cctcccggcg cacgcgcagg
     6541 cggttgagca ccactttgcg gcggcgtagc cctttgtgga gagcgattag cagcaggctg
     6601 tgtcggcgca gcaggcgcag tagctgcatt tcctgtcgcc ggttgctgcg gagtcggctg
     6661 agcgatcccc gttgccggtt caggtaactt tggccccatc gcgtctggcg aaggcaatac
     6721 ctgcggcatc atctgcatca gacgttcatt agcaacacga tgcacctctt ccggcgtcat
     6781 cgggttagcg ccgttacgat ctgcccccca cagcagcagc agataacgca aacgttgcag
     6841 ccctgcagtc ggacgcgcct gaatcacagt ataagcgcgc tgggtatcga gttttacgga
     6901 agagacaacc gcgaccggat agccttccgg gaaacgaccg cccagaccgg aagtcaccag
     6961 cacatcacca acacgaatat ccgtattcgc cggcagatgc tcaagctgca aatcatccgt
     7021 acaaccgtta ccggctgcaa ttacgcggat atcgttgcgc agcacctgga ttggcagcgc
     7081 gtgggtcgca tcacaaatca gcagcacgcg actggtcagt ttagcgacgg ccaccacctg
     7141 accaacaaca cctttgtcgc tgatgaccgg ctggccttca taaacgccat taacgctacc
     7201 tttatcgata acaacttgat cgctataagg atcgttaacc gtggagataa cctgagtcac
     7261 cattttctgc tcatcctgac gcagcgggga acccagcagc tcgcgcagac gcgcgttctc
     7321 ctgtttgtat tgtccaagca tcagcagttc actgtttttc agcaacagtt cctgacgtaa
     7381 cgcccggttt tcaagttcta attggtcacg cgaggccagc gtctgcgata cgccatccag
     7441 caattcacga ggagcattgg aaacaaagta gaaaggactg acggcggtat ccatataagt
     7501 acggatttga ctgaacgtcc ccaggcggct gtcggcaata ataatgccga gcgccaccag
     7561 caccgccaga ataaggcgaa tctgtagcga cgggccacgg ctaaaaattg gcttcataag
     7621 ttatgcgtat tctcgtatca gaccaggcag ggtaaacaga cacttcccct gcctgcatcc
     7681 gattactctt cgctgaacag gtcgccgccg tgcatgtcga tcatttccag cgctttgccg
     7741 ccaccgcgcg ccacacaggt cagcgggtct tcagcaacaa cgactggaat gccggtttct
     7801 tccattaaca aacggtcaag gttacgcagc agtgcgccac caccggtgag caccatgccg
     7861 cgctcggaga tgtcggaagc cagttccggc gggcactgtt ccagtgcaac cattaccgcg
     7921 ctcacaatac cggtcagcgg ttcctgcagt gcttcgagga tttcattgga gttcagggta
     7981 aaaccgcgtg gaacaccttc tgccaggtta cggccacgaa cttcgatttc acggacttca
     8041 tcgcccggat aagccgaacc gatttcgtgc ttgatacgtt ctgcggtggc ttcaccgatc
     8101 agagaaccgt aattacgacg cacatagttg atgatagctt cgtcgaaacg gtcaccacca
     8161 atgcgcacag aagaggagta aaccacaccg ttcaaggaga taacagcaac ttcagtggta
     8221 ccaccaccga tatcaaccac catagaaccg gtcgcttcag aaaccggcag gccagcacca
     8281 attgcggcag ccatcggttc ttcaatcagg aagacttcac gggcaccagc gccctgcgcg
     8341 gattcacgaa ttgcgcggcg ttcaacctgg gtcgcgccaa ccggcacaca aaccagaacg
     8401 cgcgggcttg gacgcataaa gctgttgctg tgcacttgtt tgatgaagtg ctggagcatt
     8461 ttttcagtca cgaagaagtc ggcgataacg ccgtctttca ttgggcgaat ggcagcaata
     8521 ttgcccggcg tacggcccag catctgcttc gcgtcatgac ctactgcagc tacgcttttc
     8581 ggtgaaccgg cacgatcctg acgaatggcc accacggaag gctcattcaa tacgatgcct
     8641 tgtcctttta cataaatgag ggtattcgca gtacccaggt caatggacaa gtcattggaa
     8701 aacatgccac gaaatttttt caacatacta agggataatc ctgaaagctg gggcggaaaa
     8761 gaaaatccgc ttactttacc aaccacacgc agcagcgaca aggcgcaaaa atcatctgct
     8821 acggtgaaaa ttagtgcagt tcgtttcctt tgttacaaat ctctgcctga gtccagaaag
     8881 gcttaatgca tcagcagcat tcctcgcctg tttgcaaccg cgtaaggtca ttcatctgca
     8941 tatgtgctgc aacaatctgg cgagcagaca agcacactcc catgagacgc agcgcgcgtt
     9001 attctacgtg aaaacggatt aaacggcagg ttaaaccgag tatctttgtg aatatttttt
     9061 cacgttagta tcaagtggct gtgaggacgc gaaaaaatcc ccttgcccgc ctgtaacacc
     9121 gcgctgaatc agggtctgcc actcgcttcg cgaacgcacg ccggtggcgt aaacctgggt
     9181 gctggtcccg gagcaggctt ccaccaggct ttgaaccagc agctggttct ccgttcgctt
     9241 ctcaatgttt ctgaccagcc ccggatggag cttgagtaac tcaacattaa gttctttgat
     9301 ccaactggta cttaccagcg tcaaaccagc ctggttgacg gctacccgta cccctaaagc
     9361 attcactaaa cgaataacag gttgtaaacg actgatatgt tgacctacat cggcctctgc
     9421 aagttcaata attatgcgtt ttcgttgtga tttttcacat tgcattaacg tatcgcgcag
     9481 ccaacgctga aaacgcgggc gaatcagcga ctcaacggta atctgaatcg ccagattttc
     9541 ctctggccag taacgcaata gtggaataag acggctgatt tgcagacggt catactcttc
     9601 cgataagcca aactgcaaga ccatcggcat atactccgcc gagctaacct cttcattacc
     9661 atcgaagatg cggcacatga gttcgcgatg atgaacctga ccttcgcgag taaccgccgg
     9721 tttttgataa aggcgcgggc cgccgcgact gagcatttgc tcgataagcg tacgccagcg
     9781 aacattaccg cgtccttttt caggcaacga gtcatcgtaa atagcccagc tattgccgcc
     9841 ctgcaatccc gcattacgcg tggcagactc tgcatgttcc attacctgct cggtatcctg
     9901 accactacgc caggcgcaga taccaatgtg gatcatatcg tcgcgatcga gcattttatt
     9961 gttcggcaag gtatcaacgg ctttgattaa ctgaccggcg atgctctctg cctcttttaa
    10021 cgtccggtgc ggtaacagcg cagcaaaatc actgcggtgg taacgcgcca gcagtgcgcc
    10081 agggtagcgc atcataaatg tcgacagcag attcgtcaga gtgaagaact gttcttcaac
    10141 ctggctgtgc ccccaggtat cgctcaacat attgaaatcc ggcagacgaa tcatcatcac
    10201 gatcccgtgg gtacctactt tctcctgatc ttccagtaac gttgctaact gattatcgaa
    10261 aaagagtcgg ttattgaggc cggttttcac gtcctgggcg gcataagagc ggatcagcgt
    10321 atcaagacgg ctgtgttgtt cgcgtgcgtt ctgaatttca cgaagcagcg tatccagcgc
    10381 actgctggtt ctgggcggcc attcatagat ggttcccaac acattagagc cacgctcacc
    10441 gtttaagata cgagtagccc gggtttccag caattcttgc ccggcaagtt gccgttgtaa
    10501 ccagcgtacc gccaggaaga gcataacaat gataaagcca atcgcccccg tgagcggcgc
    10561 ggtggtcatc aacgaatgga aatagttgcc catcggatcc tgataaacca gacgcaacga
    10621 catccccgga tgctttatca acggaacgct cagttcgcga aacagatcgc tggagccaac
    10681 tggacgataa ctaccatttc tggccagggt ataaacctgt ttatcaccat ggagcaggtc
    10741 tacacgaacg atatctgccg acatcattaa ttcggtaatt tgtggcctta atacgctgaa
    10801 gtcattcgac acaaggtggg tatcaatcgc cgtcgccacc gcctgaacgc gatgactaaa
    10861 cttatactga atggcgttgt agaaacttag cgaacagccc agcaaagtca caaaaattgt
    10921 taacccggtg agcagcgtaa caaaggccga aaatttcgtc gttaatctca tccttgtgtt
    10981 aactccgata gtgaggaagc gggcatacta gcaaatcaga tttatctcgc aatttattgc
    11041 gcttcatcgg ctttgctttt ccattagcga gtatagtctt cagaaattat tttccaatcc
    11101 atcatgcaca tgaggaccac ttatgcaggc gttactttta gaacagcagg acggcaaaac
    11161 tctcgcatca gtacagactc tggacgaaag tcgcctgccg gagggcgatg tcacggtcga
    11221 tgttcactgg tcgagcctga actataaaga tgcgctggcg attaccggta agggaaaaat
    11281 catccgtaat tttccgatga ttcctgggat cgattttgcc ggaactgtac gcaccagcga
    11341 agatccgcgt tttcatgccg gtcaggaggt gttactcact ggctggggcg ttggtgaaaa
    11401 ccactggggt gggctggcgg agcaggcgcg agtgaaaggt gactggctgg ttgccatgcc
    11461 gcaagggctg gacgcgcgta aagcaatgat tatcggtact gccggtttta ccgccatgct
    11521 gtgtgtgatg gcgctggaag atgccggtgt tcgcccgcag gacggggaga ttgtcgtgac
    11581 gggtgccagt ggtggcgtcg gcagtaccgc cgtggcgctg ctgcataagt tgggttatca
    11641 ggtcgttgcc gtttccggtc gcgaaagtac ccatgaatat ctgaaaagtt taggtgctag
    11701 ccgtgttctc cctcgtgatg agtttgccga atcccgtcct ctggaaaaac aagtctgggc
    11761 tggggcaatt gacaccgttg gcgacaaagt gctggcaaaa gtgctggcgc aaatgaatta
    11821 cggcggctgc gtggcggcct gtggtctggc gggtggtttt actctgccaa ccacggtcat
    11881 gccatttatt ctgcgtaatg tccgtttgca aggggtggat tcagtaatga cgccaccaga
    11941 acgccgcgca caagcctggc agcgactggt cgccgattta ccggaatcat tctataccca
    12001 ggcggcaaaa gagatatctc tggcagaggc accgaagttt gccgaggcca tcattaataa
    12061 ccagatccag ggtcgcacgc tggtgaaggt taactaacca tttagcaagg gataataaga
    12121 gagggaactc attttgaaat cattattcaa tcggttaacg ggaaaagcgg ttagccggac
    12181 agctttcgtc gaacatcttg gtcaggaagt tgtacaacat catccaaact ggaaagtcat
    12241 gatttcgact gaccataaat tgatgcgcat tgatacgggt caccaaacgg tggaaatcac
    12301 ccttgatgat gcctacaaac gttatcaagc cgcaccagac ccgctgcaag aagtggctgc
    12361 agatatgctg aaacagcttg cagaaatgct gccgaccggg cgctgtattg ttccggtgat
    12421 taaagaaaaa gcctggctgg ataccgttcg ccaacagctt agccagtatg agtctgatcc
    12481 agaggtcctc aacgcctcgc tggattttgc ctgggaagac tttaacgatg agttaattat
    12541 cttgtttgct gaagatggta gcgaaaccat gcgctatctg atgcgctacg aagtgaccat
    12601 tgaccgcgaa aaagccatca gtaatttgaa gcaaatattg ccggaagtta cggctgaaat
    12661 cgttgataca ggcgcaaacg atggaaaagt tgccgttatc tccgcagggg gtacttatga
    12721 gcccagtctg atgctcattg acgatctttg ggtgcaatca cccgatcctg aatgcggcga
    12781 cctgttagtg accgttccct ccagaggaaa actgtttttc acccacaatg atcagccagc
    12841 aaatctcagt gcattacgac agttaacaga acaatgcttc cgtgaggatg accatcctct
    12901 gacaacacag atattcatcc gccgtaacgg tcacttttat ccctgggtag aaaccttgca
    12961 ttaacgcaac ctccgtcatg cttgaaaaag cttgtcggag tatgtgatga aaactgcaaa
    13021 ctcaccgaag ccgattttgc taccaaatcg gcttttttat tgtcaggtgg caaattctga
    13081 aagcatcaga tagccgcact gccgcctgac aaaacagttt accttctgca aaatgggggg
    13141 aataatctga ttttgtttga ctaaaaatta atcactcgaa cctatttaat gctgagcatt
    13201 gtcaatcggt taattttgcg tgctttagca ttcacatcta tccagacgat gcagtgaaaa
    13261 ttgggtaatc cccagcaacc gctgcgtaat gtcgtctatc ttgtcgcgat cctggcatcc
    13321 ctacattatt tgtggtctgt gaagattatc tcattgcagc ccctcatctt cgcagggctg
    13381 gctttacagc ttttcacctt acgttataag aagttccgtc gatgatggcg ctaatttcgt
    13441 gaattgtgcg gcttgttgca aattacacgg tgttgaaggt tatttacatg ttagctgttg
    13501 attatcttcc ctgataagac cagtatttag ctgccaattg ctacgaaatc gttataatgt
    13561 gcgacctcgt cctccctgac gcagtttttg cgctgcggaa aaggtgacat tggcgcaacg
    13621 aaggtatatt ttgttttttg ccggaggata gcagcagatc gctgcacaat gtccgtcaag
    13681 tctaacattg acactctggg gcaaaataga ccggcgtccc ggcctgctgg aatttatcgc
    13741 tatgcataca gctgtcgggg catacgcttt acagacggcg gtgaaacgcc tgtcacaatc
    13801 acactaaaca aagagtacgg aacccactca tggatattcg taagattaaa aaactgatcg
    13861 agctggttga agaatcaggc atctccgaac tggaaatttc tgaaggcgaa gagtcagtac
    13921 gcattagccg tgcagctcct gccgcaagtt tccctgtgat gcaacaagct tacgctgcac
    13981 caatgatgca gcagccagct caatctaacg cagccgctcc ggcgaccgtt ccttccatgg
    14041 aagcgccagc agcagcggaa atcagtggtc acatcgtacg ttccccgatg gttggtactt
    14101 tctaccgcac cccaagcccg gacgcaaaag cgttcatcga agtgggtcag aaagtcaacg
    14161 tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat gaaccagatc gaagcggaca
    14221 aatccggtac cgtgaaagca attctggtcg aaagtggaca accggtagaa tttgacgagc
    14281 cgctggtcgt catcgagtaa cgaggcgaac atgctggata aaattgttat tgccaaccgc
    14341 ggcgagattg cattgcgtat tcttcgtgcc tgtaaagaac tgggcatcaa gactgtcgct
    14401 gtgcactcca gcgcggatcg cgatctaaaa cacgtattac tggcagatga aacggtctgt
    14461 attggccctg ctccgtcagt aaaaagttat ctgaacatcc cggcaatcat cagcgccgct
    14521 gaaatcaccg gcgcagtagc aatccatccg ggttacggct tcctctccga gaacgccaac
    14581 tttgccgagc aggttgaacg ctccggcttt atcttcattg gcccgaaagc agaaaccatt
    14641 cgcctgatgg gcgacaaagt atccgcaatc gcggcgatga aaaaagcggg cgtcccttgc
    14701 gtaccgggtt ctgacggccc gctgggcgac gatatggata aaaaccgtgc cattgctaaa
    14761 cgcattggtt atccggtgat tatcaaagcc tccggcggcg gcggcggtcg cggtatgcgc
    14821 gtagtgcgcg gcgacgctga actggcacaa tccatctcca tgacccgtgc ggaagcgaaa
    14881 gctgctttca gcaacgatat ggtttacatg gagaaatacc tggaaaatcc tcgccacgtc
    14941 gagattcagg tactggctga cggtcagggc aacgctatct atctggcgga acgtgactgc
    15001 tccatgcaac gccgccacca gaaagtggtc gaagaagcgc cagcaccggg cattaccccg
    15061 gaactgcgtc gctacatcgg cgaacgttgc gctaaagcgt gtgttgatat cggctatcgc
    15121 ggtgcaggta ctttcgagtt cctgttcgaa aacggcgagt tctatttcat cgaaatgaac
    15181 acccgtattc aggtagaaca cccggttaca gaaatgatca ccggcgttga cctgatcaaa
    15241 gaacagctgc gtatcgctgc cggtcaaccg ctgtcgatca agcaagaaga agttcacgtt
    15301 cgcggccatg cggtggaatg tcgtatcaac gccgaagatc cgaacacctt cctgccaagt
    15361 ccgggcaaaa tcacccgttt ccacgcacct ggcggttttg gcgtacgttg ggagtctcat
    15421 atctacgcgg gctacaccgt accgccgtac tatgactcaa tgatcggtaa gctgatttgc
    15481 tacggtgaaa accgtgacgt ggcgattgcc cgcatgaaga atgcgctgca ggagctgatc
    15541 atcgacggta tcaaaaccaa cgttgatctg cagatccgca tcatgaatga cgagaacttc
    15601 cagcatggtg gcactaacat ccactatctg gagaaaaaac tcggtcttca ggaaaaataa
    15661 gactgctaaa gcgtcaaaag gccggatttt ccggcctttt ttattactgg ggatcgacaa
    15721 cccccataag gtacaatccc cgctttcttc acccatcagg gacaaaaaat ggacactcgt
    15781 tttgttcagg cccataaaga ggcgcgctgg gcgctggggc tgaccctttt gtatctggca
    15841 gtttggttag tagccgctta cttatctggc gttgcccccg gttttaccgg ctttccgcgc
    15901 tggtttgaga tggcctgcat cctgacgccg ctgctgttta ttggactgtg ctgggcgatg
    15961 gtgaaattta tctatcgcga tatcccactg gaggatgacg atgcagcttg aagtaattct
    16021 accgctggtc gcctatctga tggtggtgtt cggtatctcg gtttatgcga tgcgtaaacg
    16081 gagcaccggc accttcctta atgagtattt cctcggcagc cgctctatgg gcggtattgt
    16141 gctggcgatg acgctcaccg cgacctatat cagtgccagt tcgtttatcg gcgggccagg
    16201 agctgcttat aaatacgggc tgggctgggt attgctggcg atgattcagc ttcctgcagt
    16261 ctggctttca ctcggtattc tcggcaagaa gtttgcgatt cttgcgcgcc gctacaatgc
    16321 agtgacgctg aacgatatgc tgtttgcccg ctaccagagt cgtcttctgg tgtggctggc
    16381 gagtttgagt ttgctggttg cgttcgttgg tgcgatgacc gtgcagttta tcggcggtgc
    16441 gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc gggctgctga tttttggtat
    16501 cagcattgcg ttatataccg cctttggtgg ctttcgcgcc agcgtgctga acgacaccat
    16561 gcaagggctt gtgatgctga ttggcaccgt tgtgctgctt attggcgtag tacatgccgc
    16621 tggcggctta agtaacgcag tacagacctt gcaaaccatc gatccgcaac tggttacgcc
    16681 acaaggcgct gacgatattc tgtcgcctgc ctttatgacg tcgttctggg tactggtgtg
    16741 ttttggcgtg attggcctgc cgcatactgc ggtgcgctgt atctcttata aagacagcaa
    16801 agccgtacat cgggggatca tcatcggtac gattgtggtc gcaattctga tgttcggtat
    16861 gcacctggcc ggagcgttag gtcgggcggt gatccccgat ctcaccgtac cggacctggt
    16921 gatcccaacg ttaatggtaa aagtgctgcc accgtttgct gccgggatct tcctggctgc
    16981 accgatggct gcgatcatgt cgacaattaa cgcccaactg ctgcaaagtt ccgctacgat
    17041 cattaaagat ctctatctga atatccgtcc ggatcaaatg caaaacgaga cgcgtctgaa
    17101 gcggatgtcg gcggtaatta cgttagttct cggcgcgttg ctgctgcttg ccgcctggaa
    17161 gccgccagaa atgatcatct ggctgaattt gttggccttc ggtgggctgg aagccgtttt
    17221 cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc aacgccaaag gcgcgctaag
    17281 tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg acgctgaata ttcagtacct
    17341 gggcttccac cctatcgtgc cctcgttact actaagtttg ctggctttcc tggtcggaaa
    17401 ccgtttcggt acatccgtcc cgcaagctac cgttttgact actgataaat aaagagtttt
    17461 gccatgcctt ggatccaact gaaactgaac accaccggcg cgaacgcgga agatcttagc
    17521 gatgcgctga tggaagcggg tgccgtttct atcacttttc aggataccca cgatacgcca
    17581 gtatttgaac cgctgccggg cgaaacgcgc ctgtggggcg acaccgatgt gattggtctg
    17641 ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc tggaaaacca tccgctgctc
    17701 ggcgcaggct tcgcgcataa aatcgaacaa ctagaagata aagactggga gcgcgaatgg
    17761 atggataatt tccacccgat gcgctttggt gaacgactgt ggatctgccc tagctggcgt
    17821 gatgtgccgg acgaaaacgc cgtcaacgtg atgttagatc cagggctggc gtttggtacg
    17881 ggtacccatc caaccacctc tctgtgcctg caatggctcg acagcctcga tttaaccggt
    17941 aaaacagtca tcgactttgg ctgtggttcc ggcattctgg cgatcgcggc gctgaaactg
    18001 ggtgcagcaa aagccattgg tattgatatc gatccgcagg cgattcaggc cagccgcgat
    18061 aacgccgaac gtaatggcgt ttctgaccgt ctggaactct acttaccgaa agatcagcca
    18121 gaagaaatga aagccgacgt ggtggtcgct aacatccttg caggcccatt acgtgaactg
    18181 gcaccgttaa tcagcgtcct gccggtttca ggcggtttgc tgggcctttc cggtattctg
    18241 gcaagccagg cagagagcgt ttgtgaagct tatgccgata gcttcgcact ggacccggtc
    18301 gtggaaaaag aagagtggtg ccgtattacc ggtcgtaaga attaaccttc gcatcgccgt
    18361 agggtgacgc ggggcgaagt gcgagcaagc tcacaaaagg cacgtaaatt tgccgattat
    18421 ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa aaattgagaa cttactcaaa
    18481 tttctttgag tgtaaatttt agtcactatt ttctaatatg atgattttta tgagtaatta
    18541 tcgcaccacg ctcattttaa atgcaattct ttgatccatc tcagaggatt ggtcaaagtt
    18601 tggcctttca tctcgtgcaa aaaatgcgta atatacgccg ccttgcagtc acagtatggt
    18661 catttcttaa ctcatgcgca tcggacaata tcagctcaga aatcgcctga tcgcagcgcc
    18721 catggctggc attacagaca gaccttttcg gacgttgtgc tacgagatgg gagccggatt
    18781 gacagtatcc gagatgatgt cttctaaccc acaggtttgg gaaagcgaca aatctcgttt
    18841 acggatggtg cacattgatg aacccggtat tcgcaccgtg caaattgctg gtagcgatcc
    18901 gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc ggtgcccaga ttattgatat
    18961 caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc gcaggttcag ccctcttgca
    19021 gtacccggat gtcgttaaat cgatccttac cgaggtcgtc aatgcagtgg acgttcctgt
    19081 taccctgaag attcgcaccg gctgggcacc ggaacaccgt aactgcgaag agattgccca
    19141 actggctgaa gactgtggca ttcaggctct gaccattcat ggccgtacac gcgcctgttt
    19201 gttcaatgga gaagctgagt acgacagtat tcgggcagtt aagcagaaag tttccattcc
    19261 ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc agagctgtgc tcgactatac
    19321 aggggcggat gccctgatga taggccgcgc agctcaggga agaccctgga tctttcggga
    19381 aatccagcat tatctggaca ctggggagtt gctgcccccg ctgcctttgg cagaggttaa
    19441 gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt tatggtccgg caaaagggta
    19501 ccgaattgca cgtaaacacg tttcctggta tctccaggaa cacgctccaa atgaccagtt
    19561 tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag ctggaggcgt tggaggcata
    19621 cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag aactatgttc gaacaacgcg
    19681 taaattctga cgtactgacc gtttctaccg ttaactctca ggatcaggta acccaaaaac
    19741 ccctgcgtga ctcggttaaa caggcactga agaactattt tgctcaactg aatggtcagg
    19801 atgtgaatga cctctatgag ctggtactgg ctgaagtaga acagcccctg ttggacatgg
    19861 tgatgcaata cacccgtggt aaccagaccc gtgctgcgct gatgatgggc atcaaccgtg
    19921 gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta aagtcaagct gtaacggcat
    19981 gaaattaaag gggctatttc agtcccttta ctttttgttg caaccgtact gacaaccctt
    20041 atggtgtttg cgcgccatct atcatcatgc ttcgctgcct tactgcttgt tgctgtcgta
    20101 gttgctgtat caccttccgg gctaatgcat cttcattcat accaggttct gccttcacgc
    20161 tgatgtggta ctggtcgttg ctatgcaccg tagtgccacc agagcggttg gccagtgcag
    20221 gtgacggcag ggcagcacca gaggcagtgg ttgctgctgg ctgctgtaca tcgtcgcgga
    20281 atggtgcggt aaagaaatct ttggttttct gccacgcacc accagcaaca tcagacacgg
    20341 cgtttttaaa ctccagtgcc ttgttggtca ggttgccgat gttgtcagac acccacagaa
    20401 tcttatcaat caggaacgtg atcggtgcgg tcacaagctc gatgccagcc gccagggtct
    20461 taccgaacgt catccccatt ttaccggcct tatccagttc ctcgccactg tacttgaccg
    20521 gctcggtcaa tgacgtgaac cagttgaaaa ccgtcttaac cgcagcaccc acaccatcaa
    20581 tgacaggttt cagcggcccc atgcgttcat aaaggcggct cagagcgtcg ctaagcggtt
    20641 ccagcccgga tttgatgcct tcacccactc cgccaaagaa agccttcaca ccagcccaat
    20701 ggcggtaaat ctgataacct gccagcgcca ggccagctac ggcagcaccc acgccagtgg
    20761 atatgaacgc cgcaccaacg gcacgaatgc cggtagccac cgcaggcagg ccacgagtgc
    20821 tggccagaac ggcagatgcg ttcatgcggg caaacaggct ggacatctgc aacactccac
    20881 ctttcaggaa agtgaagccg aactgcgccg ccagggtggc cacacgcagg gacgtgagcg
    20941 ccatcgctgt gcccaccacg gctttggtca ggccagggtg ttccgccgcg aagttcgcca
    21001 caacagttgt gatgcggcca atgcctttgg ctgcgtcatt gacggtcggt agcaggacgg
    21061 tgccgatatt gtcagccacg tcagctgtgc ggttacgaag gataaccatc tgcgcgttgg
    21121 tggtgttgac ggtgttctgg aattccttct gcacactgcc tttggacatt tcgccattag
    21181 cctggtgcaa cgcctcgcca aacttgtcca tgttggctgc cagtttggag atagaaccaa
    21241 cgtgttcctg cccgaagatg gacaccagga tgccggattt cttgtaatct ggcagcttgt
    21301 tcaccgtctt catgaagttt tcgatggtct tgacgctatc cagttgcatg gatttcgcca
    21361 cttcctgcgc ggaataaccc aattccttga acgcttcgcg cgcagatttc gtggatttgc
    21421 tgcccagcgt catcttggaa acaagcgcct tcactgacgt tgcagcagtg tctgcgcttt
    21481 cacccataga aatcagcgtg gtgccgattg ctgccatatc gttggcagtg agtttggcga
    21541 gggaggcgga accaccaaca cgctgtagga agtcgataag ctcaccgcct ttaacggtgt
    21601 tttggtcgtc caggtaatta atagcatcac caaggcgcac aagctcagct tgagtaagtc
    21661 gataaacatt ctggattttg cccatattgt ctgcgacttc ttccgggttc agttccaagg
    21721 cgctggccag tttggccacg ttccgggtga accccaggat ttggtcgcca gctacgccca
    21781 tgcgcagccc tgctgtggcc atgtcaaaca ggtcgtttgt ggccagtgga atttcgtgcc
    21841 caaggtcagc aacggccttg cgcatttccc aaaacttcgc cgtcaggttg cccgctgcat
    21901 cgcgtgcgcc gtccagttgt ttggcaatgc caatggcgtg ctgttcacga tcggcagcaa
    21961 tcttgatggg tgcggcaacg gcagcgccag ccataacggt atcgataatc tgcccgcgta
    22021 gctgtgcctg cttcgccagg ttttccgcac tggcgcgttg cactctgttt aaacgctctt
    22081 gcgcagcgcg aaggcgttcc acctggccga caacagcctg gtaacgctct ttcatgcgcc
    22141 cgaccattgc accatcctgc gcataacgct tcatagcgtc gttaagctgg cgctgttcgc
    22201 gcgacatgtg cttaatcgct gcgccaatct ttatggtgga tttctccacc gtaccaaacg
    22261 caccacggag gctgccggaa acggcacccc cgatggtgat tgtggcgttc aacttcttat
    22321 tggccataat aatttgtctc tgaaagaatt aattaagtag attttctgcc agggtgtgtt
    22381 caccagcagc ccgcagggcg gcagcagcca gttgcagggc gcattcggtg cgatgtttac
    22441 gctgtgcctg cattccctcc gctgtttcgc aaaccataac tttgggcagg accatatcca
    22501 ccagttcagg ccaggggttt gttccttccg gttttgtgtg aatacggtcc agcatggtgc
    22561 ccccttatgc ggttttgctt tctgttggtt tgtcgttggc ggtgatgtcg cgcatcccaa
    22621 acgtgatacc tttccaggac ttcatcgcgc cgctaaggtt cttgagcgcc aacttttgtg
    22681 cgtcggtcag ttggcttgcg ttgttcttcg tcatcttgtc ggctacgccg atgacgttca
    22741 gaacggcttc ataaatgttg ttgttggttg ccattcttat gcgctccgca ccaggatagc
    22801 gccgcgcttc actgcctggc gcttggcccg ttcaccggaa ctgatgcggc tggatggttg
    22861 ccacttcttg cgttccaggg tggatgcgtg gctttccgct tcgtcacgca tgatgcggcc
    22921 cgcttcatcg gtcatgtgtt cgaatgcgtt taaacggtta cgaagacttg ccagcaggtc
    22981 ggctgcttcg tagctgatag ggtttttctc tgaaatggtt tggaaacgct ttgtttcgct
    23041 aaggatcaaa cgcgcttctt ctttcattga ttcgtacagg tcatggtttt tcatagtatt
    23101 ggccgctaac aatagttgtt gatgattaag tgagtgcgcg cttttaggtg accggtgcgg
    23161 cgcaaccggt gtggaattag tggtggtttt gaccgtccag gatgaccacc aacctggttc
    23221 cagcatccat cgtctgagga acgatgaagg ttaaatttgg ccggttattt ggggcacagg
    23281 gaaccggtga aacctgtatt cttggccatt atttgccgtt cagcccgcta ctcagaaact
    23341 tttcaatcac ctggctgcgt gaagggcgga tgcctgttct gcgttccagt ttggcttgct
    23401 ctctttcgac ctggcgcacc aggtcagggg cgaggcgtac cgttacgtgc tggcgtgttt
    23461 cttgtcgttt catggcttag tacctgttca tccagctttc gcgcttctgt ttcgggcgct
    23521 gctcgcggta gtgactgcgc accagggaag cgaggtaatt cacggcgcgt tcaatctggt
    23581 actgcttaag ctccgggtaa gccttgcgaa tccatttttc agcagcgtca tcgctgtaga
    23641 tgaatcctga ccagccaacc ggctgcacta cgatgcgcgg tgtgttttca tcaccgtggc
    23701 gcacatacat ggccacacct tcgatagctg ccaggatttc acccgtcagg gcagcgcgct
    23761 ccgctgccgc ttttttgctg ccccaccaca tgatcaagcc ttccagaaca tagttgagtc
    23821 aggcaccagt gcggatgcgt tcacccaaac caccacttca accgcaccat tcagcacgcg
    23881 tctggcggtg acgcgaattt catcatccag ggcaacggct gcattgccga aagcgccaat
    23941 gattgtgctg gaagtctggc cgctgatttc agcgcgtaca ccgtaggcac gcagaacgcc
    24001 gtcttgcagt tccaggccgg tgcaatcacc gccaacaatg gctttaactt catcaaagcg
    24061 caggtttttg gctgcaatct tctgtgccac tgctgtgaat gacgggctgg taaggttttc
    24121 cgctacgctt tccaggtggt tcagcagtac gaagtcagaa agatcagcta tgccgcgctc
    24181 aatggcggtg ttgaccactt ccagcgtggt atcgtctgcg aagtcgtgtt tcatctgctt
    24241 gcggctaagg gtgtagccaa cgccgtaagc tggtgcgttg ttgtggttga acgctgatac
    24301 caggtaaggc aggccgctca tggtgatttc ttcgccgtca gccactttgg cgaacttgcc
    24361 agggcggatg acaacgaagc ggtcagccac ttcgcggcca gtcgttgctc cggttttgcc
    24421 ggtcggttgc gtggtcatcg ggccaggaat gcggatgatt tcagcgcctg cctgtgccac
    24481 cagtgaacgt tcagccagca cagcgttgat ggttgtctgt ttgccgcctt tcggggataa
    24541 aacgtcattc atggacaggt agcgaactgc tgcaggttcc acagggatga attgagtaat
    24601 ttcaccgttg gcgtcagtgc gaatgtggcc gccctggtgg ttcaacgcgt cgttgataag
    24661 ttgagataaa acgttcatta aaaatgctcc ggttgttgtg caataaatcg tctggaatga
    24721 cttaaagata tac
//
LOCUS       DACAPB010000052        24455 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.052, whole genome shotgun sequence.
ACCESSION   DACAPB010000052 DACAPB010000000
VERSION     DACAPB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.052"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..258)
                     /locus_tag="IT904_001905"
     CDS             complement(<1..258)
                     /locus_tag="IT904_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191692.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTG"
     gene            complement(281..1474)
                     /gene="acrA"
                     /locus_tag="IT904_001906"
     CDS             complement(281..1474)
                     /gene="acrA"
                     /locus_tag="IT904_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAP3191693.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            1616..2263
                     /gene="acrR"
                     /locus_tag="IT904_001907"
     CDS             1616..2263
                     /gene="acrR"
                     /locus_tag="IT904_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAP3191694.1"
                     /translation="MARKTKQEAQETHQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            2391..5753
                     /gene="mscK"
                     /locus_tag="IT904_001908"
     CDS             2391..5753
                     /gene="mscK"
                     /locus_tag="IT904_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAP3191695.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAAG"
     gene            complement(5965..6126)
                     /gene="rsmS"
                     /locus_tag="IT904_001909"
     CDS             complement(5965..6126)
                     /gene="rsmS"
                     /locus_tag="IT904_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAP3191696.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(6140..6667)
                     /gene="priC"
                     /locus_tag="IT904_001910"
     CDS             complement(6140..6667)
                     /gene="priC"
                     /locus_tag="IT904_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAP3191697.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLVRVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            6737..7114
                     /locus_tag="IT904_001911"
     CDS             6737..7114
                     /locus_tag="IT904_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAP3191698.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            7267..7818
                     /gene="apt"
                     /locus_tag="IT904_001912"
     CDS             7267..7818
                     /gene="apt"
                     /locus_tag="IT904_001912"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP3191699.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            7947..9878
                     /gene="dnaX"
                     /locus_tag="IT904_001913"
     CDS             7947..9878
                     /gene="dnaX"
                     /locus_tag="IT904_001913"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP3191700.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRTSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     gene            9931..10260
                     /locus_tag="IT904_001914"
     CDS             9931..10260
                     /locus_tag="IT904_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP3191701.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            10260..10865
                     /gene="recR"
                     /locus_tag="IT904_001915"
     CDS             10260..10865
                     /gene="recR"
                     /locus_tag="IT904_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP3191702.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            10975..12849
                     /gene="htpG"
                     /locus_tag="IT904_001916"
     CDS             10975..12849
                     /gene="htpG"
                     /locus_tag="IT904_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAP3191703.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            13030..13674
                     /gene="adk"
                     /locus_tag="IT904_001917"
     CDS             13030..13674
                     /gene="adk"
                     /locus_tag="IT904_001917"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP3191704.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            13910..14872
                     /gene="hemH"
                     /locus_tag="IT904_001918"
     CDS             13910..14872
                     /gene="hemH"
                     /locus_tag="IT904_001918"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAP3191705.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(14869..15828)
                     /gene="aes"
                     /locus_tag="IT904_001919"
     CDS             complement(14869..15828)
                     /gene="aes"
                     /locus_tag="IT904_001919"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAP3191706.1"
                     /translation="MKPENKSPVLDLISAGMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            15980..17284
                     /locus_tag="IT904_001920"
     CDS             15980..17284
                     /locus_tag="IT904_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAP3191707.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(17417..19093)
                     /locus_tag="IT904_001921"
     CDS             complement(17417..19093)
                     /locus_tag="IT904_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAP3191708.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGVAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(19331..20551)
                     /locus_tag="IT904_001922"
     CDS             complement(19331..20551)
                     /locus_tag="IT904_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191709.1"
                     /translation="MAMSEQPQPAAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            20769..22421
                     /locus_tag="IT904_001923"
     CDS             20769..22421
                     /locus_tag="IT904_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAP3191710.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVCFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(22458..22937)
                     /gene="ybaK"
                     /locus_tag="IT904_001924"
     CDS             complement(22458..22937)
                     /gene="ybaK"
                     /locus_tag="IT904_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAP3191711.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(23141..23935)
                     /locus_tag="IT904_001925"
     CDS             complement(23141..23935)
                     /locus_tag="IT904_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191712.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            24073..24414
                     /locus_tag="IT904_001926"
     CDS             24073..24414
                     /locus_tag="IT904_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP3191713.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERTKKVA"
BASE COUNT         6082 a         6297 c         6454 g         5622 t
ORIGIN      
        1 acccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg
       61 ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc
      121 ggagatcgtt actgccggcg gcgcaatcgt aggatattgc gccaccggca gtttgaggat
      181 cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc
      241 gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag
      301 gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct
      361 ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac
      421 cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca
      481 ctttgtcatc cgcgccaact accagtacgg tggcatcgcc acgcggcgta cgggttacgc
      541 cctgttgcgg gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga
      601 acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc
      661 cagtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa
      721 tgccgtcact ggtgatcagt gacactttgg ctttgccgtt ctcttgtttc agcgtgccat
      781 tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga
      841 tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc
      901 cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agaggtgact ttggtgtaag
      961 ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct
     1021 gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca
     1081 gtttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca
     1141 gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag
     1201 agacacctgc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa
     1261 cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga
     1321 tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt
     1381 gttgggcctg tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga
     1441 ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca
     1501 aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta
     1561 tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc
     1621 acgaaaaacc aaacaagaag cgcaagaaac gcaccaacac atcctcgatg tggctctacg
     1681 tcttttctca cagcaggggg tatcatccac ctcgctgggc gagattgcaa aagcagctgg
     1741 cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat
     1801 ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc
     1861 tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac
     1921 agaagaacgg cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga
     1981 aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga
     2041 acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg
     2101 cgcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc
     2161 gcaatctttt gatcttaaaa aagaagcccg cgattacgtt gccatcttac tggagatgta
     2221 tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca
     2281 ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg
     2341 cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt
     2401 tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg
     2461 tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa
     2521 aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg
     2581 acaaactggt gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgca
     2641 taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc
     2701 aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa
     2761 ttctgagcac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt
     2821 tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc
     2881 agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca
     2941 gtcgtctgga tgggactgat gtcggcgaga cagccttacg tcccagccag aaagtgttaa
     3001 tgcaggccca gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag
     3061 ggaacaccgt cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg
     3121 ctcgtctgga gcaccagtta caactgttgc aagaagcggt aaacagcaag cgcctgactt
     3181 taaccgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta
     3241 atccgctggt gaagcaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg
     3301 cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc
     3361 gggcgctaca atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc
     3421 tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa
     3481 acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg
     3541 atgcactctt ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag
     3601 tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg
     3661 atcaactcaa caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc
     3721 agcagcagtt aatgagtgtg tcgaaaaacc tgaaatccat cctgactcag caaatctttt
     3781 gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga
     3841 aagatgaatt taagtcgatg aaaatcacgg tgaactggca aaaagcctgg cccgccgttt
     3901 ttatcgcttt cctcgctggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc
     3961 tgggctggct gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc ctgcgtaacg
     4021 acagccagct caacacacca aaagcgatcc ttatcgacct gatccgtgcg ctgccggtgt
     4081 gcctgattat tctcgcggtt ggcctgattc tgttgaccat gcagctcaac atcagcgaac
     4141 tgctatggtc gttcagcaaa aaactggcga tattctggct ggtgtttggc ctgtgctgga
     4201 aggtactgga gaaaaacggc gttgccgtac gtcacttcgg catgccggaa cagcagacca
     4261 gccactggcg tcggcaaatt gtccgcatca gtctcgcatt gctgcctatc catttctggt
     4321 ctgtggtggc agaactttcc ccgctgcatc tgatggatga tgtgctgggg caagcgatga
     4381 ttttcttcaa cctgctgctg attgccttcc tggtatggcc gatgtgccgc gaaagctggc
     4441 gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg ataatcccga
     4501 ttgcgctgat ggtgctgact gctacaggct acttctacac tacgctgcgt ctggcaggac
     4561 gctggattga aaccgtttat ctggtgatca tctggaacct gctgtaccag acggtactgc
     4621 gtggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga
     4681 atctggtgaa agagggcgca gaaggtgctg aaccgccgga agaacccacc attgcactgg
     4741 agcaagttaa ccagcaaacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg
     4801 tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatctc gacagcatca
     4861 cgctctggca ttacaacggc actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg
     4921 gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc
     4981 ctggtttact ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg
     5041 ccattactac catccttaac tacatcatta ttgctgttgg tgcgatgacg gtgttcggat
     5101 cgctgggcgt ctcttgggat aaactccagt ggctggccgc agcattatcc gtaggtcttg
     5161 gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctattcgaac
     5221 gtccggtgcg tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga
     5281 tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca
     5341 aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg
     5401 tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga
     5461 aggcggcgac tgagcaccca agagtgatgc acgaaccaat gccggaagtc ttctttacgg
     5521 cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc
     5581 gtagtcgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca
     5641 tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga
     5701 cggaagtaaa acgcgactac aaaggcgacg atccgacacc agcggctggg taataaccaa
     5761 agggggcgac atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat
     5821 gcggcgtgac cgccttatcc ggcctacgaa accgcaagaa ttcaatatat tgcaggagcg
     5881 gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg
     5941 acatttagcg gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt
     6001 ttcataatcg cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt
     6061 ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttcaag
     6121 tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca
     6181 gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt
     6241 gctgttccac gagatcggtc acccgcacca ggcgggctct gcgttcggca accatctcac
     6301 gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg
     6361 gtggcgcact gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca
     6421 gttgtgccgc cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg
     6481 catgacgaag cgcagccaga ttatcgcccg cctcgtcgag acaagcctgt agtgttgtcg
     6541 cacgagtctg aaaaagatgc ctgtcgaaac gagcacttag cgtggcgaac tgtgacaccg
     6601 gggcacaacg ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg
     6661 ttttcacgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa
     6721 gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt
     6781 gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc
     6841 ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg
     6901 gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc
     6961 gcgggcgatt ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc
     7021 atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat
     7081 tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat
     7141 tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa
     7201 caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat
     7261 acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt
     7321 caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg
     7381 aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc
     7441 aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg
     7501 ggcgttggct ttgtgccggt ccgtaaaccg ggcaaactgc cgcgtgaaac tatcagtgaa
     7561 acttacgatc tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg
     7621 ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc
     7681 gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg
     7741 ttcgatctcg gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc
     7801 ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag
     7861 atgtgcattc agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc
     7921 accttccagc gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc
     7981 cacaaacctt tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct
     8041 tgtcgttagg gcgtattcat catgcttatc ttttttccgg cacccgtggc gtcggaaaaa
     8101 cctctatcgc ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc
     8161 cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg
     8221 aaatcgacgc cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc
     8281 agtacgctcc ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt
     8341 cgcgccacag ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt
     8401 ttctgctggc gacgaccgat ccacagaaat tgccggtgac gattttgtca cgctgtctgc
     8461 aatttcatct caaggcgctg gatgtcgagc aaattcgcca tcagcttgag cacatcctca
     8521 acgaagaaca tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag
     8581 gcagcctgcg agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg
     8641 tttcaaccca ggcggtcagt gcgatgctgg gtacgcttga cgacgatcag gcgctgtcgc
     8701 tggttgaagc gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg
     8761 ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat gctcggtctg ttgcatcgta
     8821 ttgcgatggt acaactttcg cctgctgcac ttggcaacga catggccgcc atcgagctgc
     8881 ggatgcgtga actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc
     8941 tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga
     9001 cgctgctgcg cgcgctggca ttccatccgc gtatgccgct gcctgagcca gaagtgccac
     9061 gccagtcctt tgcacctgtc gcaccaacgg cagtaatgac gccaacccag gtgccgccgc
     9121 aaccgcaatc agcgccgcag caggctccga ctgtaccgct cccggaaacc accagccagg
     9181 tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg
     9241 aaccggcagc cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa agactggctt
     9301 cggtcaccga tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcta
     9361 aaaaagaagc gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa gaagtggtcg
     9421 ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga
     9481 agctggcggc agaagccatt gagcgcgacg cgtgggcggc acaggttagc caactttcgc
     9541 taccaaaact ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag
     9601 tatgtctgca tttgcgcacc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac
     9661 tggctgaagc gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg
     9721 ataatcccgc ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa gaaaaacttg
     9781 cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt cgattcttcg
     9841 atgcggagct ggatgaagac agtatccgcc ccatttgatc gtaagcacag cttacgttcg
     9901 tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg
     9961 atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag
    10021 ctggaagtta ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac
    10081 aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa
    10141 gacctggtgg ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa
    10201 aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga
    10261 tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgtctg ccgggcgttg
    10321 gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga
    10381 tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc
    10441 gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg
    10501 gtcaaatctg cgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt
    10561 tttcaggtcg ttattttgtg ttgatggggc atctgtcacc gctggacggc atcggtccgg
    10621 atgatatcgg gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga
    10681 tcctcgccac caaccccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt
    10741 gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg cgttccggtt ggcggcgagc
    10801 tggaaatggt cgacggcacc acgttgtcac actcccttgc cgggcgtcat aagattcgtt
    10861 tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca
    10921 tctctcccac atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa
    10981 ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat
    11041 tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg
    11101 gcggacaagc tgcgtttccg tgcgctctct aacccggacc tgtacgaagg tgatggcgaa
    11161 ctacgcgttc gtgtctcttt cgataaagac aagcgtacgc tgaccatctc cgataacggc
    11221 gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc
    11281 aaatcattcc tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag
    11341 tttggtgttg gtttctactc tgcgtttatc gtggccgaca aagtgaccgt gcgtactcgc
    11401 gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat
    11461 accgttgccg acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa
    11521 ggcgaagacg agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac
    11581 catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc
    11641 tcctgggaga aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc
    11701 gatgaagagt acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc
    11761 tggagccaca accgtgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc
    11821 caggctccgt gggatatgtg gaaccgcgat cataaacacg gcctgaaact gtatgttcag
    11881 cgtgtgttca tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt
    11941 ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc
    12001 acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa
    12061 ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg
    12121 aaagaaggtc cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt
    12181 gcttctaccc ataccgattc ttctgcgcag accgtatctc tggaagacta cgtttcccgc
    12241 atgaaagaag ggcaggagaa aatctactac atcaccgcag acagctatgc ggcagcgaag
    12301 agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac
    12361 cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg
    12421 gtgtctaaag ttgacgagtc gcttgaaaaa ctggctgacg aagttgatga gagcgcgaaa
    12481 gaagcggaga aagcactgac tccgttcatc gaccgtgtga aagccctgct cggcgagcgc
    12541 gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac
    12601 gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca
    12661 gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat
    12721 actgaagatg aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg
    12781 gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg
    12841 gtttcctgat gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct
    12901 gcactttccg caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc
    12961 aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg
    13021 attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct
    13081 cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct
    13141 gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa
    13201 ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc
    13261 cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa
    13321 gaagcgggca tcaatgttga ttacgttctg gaatttgatg taccggacga actgatcgtt
    13381 gaccgtatcg tgggtcgccg tgttcacgcg ccgtctggcc gtgtttatca cgttaaattc
    13441 aatccgccga aagtagaagg taaagacgac gttaccggtg aagagctgac cacccgtaaa
    13501 gatgatcagg aagaaaccgt acgtaaacgt ctggttgaat atcatcagat gactgcgccg
    13561 ctgattggct actattcaaa agaagctgaa gcgggtaata ccaaatacgc gaaagttgac
    13621 ggcaccaagc cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaattcgaa
    13681 agcgcgcacg gacagtcccc tcgccccctc ggggagaggg ttagggtgag gggaacaggc
    13741 ccgcacaagc aaacttatca gcaatctcag gccggatatt cattcggcct tttacaaaaa
    13801 cctcaatctg cgctattcct ttttctgatt tgacctctca cagcaattag ttcttcttcc
    13861 tcacttttcc gctacaatta tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac
    13921 taaaaccggt atcctgctgg caaacctggg tacgcccgat gcccccacac ctgaagcggt
    13981 aaaacgctat ctgaaacaat ttttaagcga cagacgcgtg gttgatacct cacggttgtt
    14041 atggtggcca ttgctgcgcg gcgtgatttt gccgctgcgc tcgccgcgtg tggcgaagct
    14101 gtatgcctct gtctggatgg aaggtggctc gccgctgatg gtttacagcc gtcagcaaca
    14161 gcaggcgctg gcacaacgtt taccggagac gcccgtagcg ctgggaatga gctacggctc
    14221 gccatcactg gaaagcgccg tagatgaact cctggcagag catgtagatc atattgtggt
    14281 gctgccgctt tatccgcaat actcctgttc aacggtcggt gcggtatggg atgaactggc
    14341 acgcattctg gcgcgcaaac gtagcattcc ggggatatcg tttattcgtg attacgctga
    14401 taaccacgat tacattaatg cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg
    14461 cgaaccggat ctgctgctgc tctcttatca tggcattccc cagcgttatg cagatgaagg
    14521 cgatgattac ccgcaacgtt gccgcacaac gactcgcgaa ctggcttccg cactggggat
    14581 ggcaccggaa aaagtgatga tgacctttca gtcgcgcttt ggtcgggaac cctggctgat
    14641 gccttatacc gacgaaacgc tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt
    14701 gatgtgcccg ggctttgctg cggattgtct ggagacgctg gaagagattg ccgagcaaaa
    14761 ccgtgaggtc ttcctcggtg ccggcgggaa aaaatatgaa tatattccag cgcttaatgc
    14821 cacgccggaa catattgaaa tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc
    14881 ggtaaagaac tgagcgccgt cgcgaagagc ctcgtcggcg gttttcatca tccgtgaata
    14941 atgcaaaaag gcgtgcagcg tgcctgggta gagtttgaac tcacagggct gctgatgcgc
    15001 cgctaacgtc tggtaaagca gacggctgtc atccagcagc ggatcgaact ccgccccggc
    15061 aataaaacag ggcggaactt cgcgagtgag atcattatta aacagacagt aatacggcga
    15121 ctcgcggtcc gcgtcgttgc ttaaatacgc ctcttcatac atctgcaaat cctgttgcgt
    15181 taagccatcc cagacaccgc ccaacagacg acgagtcacg gaatcccgta atccgtaaag
    15241 cccataccac agcaaaacac ccgcaatttt accgcaatcg atctgtttat cacgcaacca
    15301 caacgcactg gcgagcgcca gcatggcacc tgcggaatca ccggcaaagc caatgcggga
    15361 catattgatt tgataatcct ccgcctgctg gtggaaataa caacaagcag ccacaatttc
    15421 ctctatcgct tgcggaaaac gcgcttcagg tgaaagggtg taatcaatac caatcaccgt
    15481 acattggctg tagcttgcca gcaggcgcat gatgcgatcg tgggtatcga gattgccgag
    15541 aataaaaccg cctccatgca aataaaatag cgtcgccggg ctatctggct gcggacaaaa
    15601 gagacgtgtt tccacctgcc catattttgt tggaaccatg taagctctgg ttgccatttc
    15661 tggagcgccc gcattccaga atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat
    15721 cgttcccgtt gcgggccagg gcggtaaatc cggctgaaga gtattcacaa cggtcttcat
    15781 tccagcagaa ataaggtcca gaacaggtga tttgttttcc ggcttcatac aaaaactcct
    15841 ttcaaattac gtcattgtaa ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat
    15901 tgaaactggc tgcgtctcgc gcgctcccgt cagattgtgt taacattcgc cgctcagtta
    15961 accacccgta aaaacaacca tgaaatttcc cggtaaacgt aaatccaaac attacttccc
    16021 tgtaaatgca cgcgatccgc tgcttcagca gttccagcca gaaaacgaaa ccagcgccgc
    16081 ctgggtagtg ggtatcgatc aaacgctggt cgatattgaa gcgaaagtgg atgacgaatt
    16141 cattgagcgt tatggattaa gcgccgggca ttcactggtg attgaggatg acgtagccga
    16201 agcgctttat caggaactaa aacagaaaaa cctgattacc catcagtttg cgggtggcac
    16261 tattggtaac accatgcaca actactcggt gctcgcggac gaccgttcgg tgctgctggg
    16321 cgtcatgtgc agcaatattg aaattggcag ctatgcctat cgttacctgt gtaacacctc
    16381 cagccgtacc gatcttaact atctacaagg cgtggatggt ccgattggtc gttgctttac
    16441 gctgattggc gagtccgggg aacgtacctt tgctatcagc cctggccaca tgaaccagct
    16501 gcgggctgaa agtattccgg aagatgtgat tgccggagcc tcggcactgg ttctcacctc
    16561 ttatctggtg cgttgcaagc cgggtgaacc catgccggaa gcaaccatga aagccattga
    16621 gtacgcgaag aaatataacg taccggtggt gctgacgctg ggaactaagt ttgtcattgc
    16681 cgagaatccg cagtggtggc agcaattcct caaagaccac gtctctatcc ttgcgatgaa
    16741 cgaagatgaa gccgaagcgt tgaccggaga aagcgatccg ttgttggcat ctgacaaggc
    16801 gctggactgg gtagatctgg tgctgtgcac cgccgggcca atcggcttgt atatggcggg
    16861 ctttaccgaa gacgaagcga aacgtaaaac ccagcatccg ctgctgccgg gcgctatagc
    16921 ggaattcaac cagtatgagt ttagccgcgc catgcgccac aaggattgcc agaatccgct
    16981 gcgtgtatat tcgcacattg cgccgtacat gggcgggccg gaaaaaatca tgaacactaa
    17041 tggagcgggg gatggcgcat tggcagcgtt gctgcatgac attaccgcca acagctacca
    17101 tcgtagcaac gtaccaaact ccagcaaaca taaattcacc tggttaactt attcatcgtt
    17161 agcgcaggtg tgtaaatatg ctaaccgtgt gagctatcag gtactgaacc agcattcacc
    17221 tcgtttaacg cgcggcttgc cggagcgtga agacagcctg gaagagtctt actgggatcg
    17281 ttaagttatc gtcggttcgt aggccagata aggcgttcac gccgcatctg gcatttggct
    17341 ctcgatgcct gatgcgacgc tggcgcgtct tatcatgcct acatattttt catattttac
    17401 atccggcaac caccgtttac cccgtcacca cctcacccgc cggtggcgtt tccagcagtt
    17461 ccagcatggt acgggcgatt tcacgctcgc ccatcactac ctgattcgca ccacgttcgg
    17521 tgatatacgc cacttcatcg tcataatggg cgcgggcaat aatctcaata tccggatttt
    17581 tcgcgcgggc agatgccaca atctcacccg cttcataacc gttgggaatc gtcaggatca
    17641 gccattttgc acattccaga tgcgccagtt gcataatttc ttcgttcgcc gcattgccca
    17701 atactgcgcg gaccccgcgc tctcgcagct catcaacacg ggttcgtgac gtctcaatca
    17761 ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct acacgaccgt
    17821 aacccaccag tagcgcatgg ttgcaaatat ccactgggat ctgcttctct tcttcgattg
    17881 cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttc tccagtagtg
    17941 cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc aggttttgtc
    18001 cggcctgcgg cagtaaattc aatgccattc ccagtcccgc caggataaac gcgaactcac
    18061 caatctgcgc caggctggcg gcgatggtta atgccgtacg ttgggagtga ccaaacagtc
    18121 gcaccaggaa aaatgcggct aacgacttac caaacagaat aatcgccagc gtcgccagca
    18181 ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgacg gagacaaaaa
    18241 acagcaccgc aaacgcgtcg cgcaatggca gcgtatcgtg ggcggcacgg tgactcagtt
    18301 cagactcgtt cagtaccatc ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca
    18361 gctctaccgc accaaaggca acccctaacg ccagcgccag caccgacagg gtaaacagct
    18421 cgcgagaacc ggttgccgcg ctgcgtgcca taatccacgg caccagacgg cgacctacca
    18481 gcatcataat ggcgataaat gcgatcactt tgccgatggt gatccccata tcgactgcaa
    18541 gagtggcaaa gcccacatcg ccctgttcca tcattcctgc cactgcgggc agcaacacca
    18601 gcgtcagaac cattaccagg tcttccacaa tcaaccaacc gatggcgatt tgcccacgct
    18661 gactgtcaat taattgccgt tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg
    18721 aaagacataa accgaacacg ataccggtca ttaacgacca gcccagcacg gcagagagcg
    18781 ccatacccag cagcgtcgcc acggctatct gggcgatcgc accgggaatg gcgatggcct
    18841 ttaccgccat caaatccttc agcgaaaagt gcaaaccgac gccaaacatc aacagaatga
    18901 cgcccagttc agccagttcc ggcgcaagct tggtatcggc aacaaagccc ggagtgaatg
    18961 gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt agtttattgg
    19021 ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg ataagcgggg
    19081 tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg gccgggaaaa
    19141 ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgttcatga ataattattg
    19201 aattttgcag aaaaatggaa ttagctgcaa aaaaagcacg gatcggaaaa cagaaggcgg
    19261 tttaacagag gaaaggctta tggcgactgt aaaacgatgc agccaaagtt tgcctttggc
    19321 tgcaatgaaa tcagtcttta tgccggttat caggcaggaa tatggtcaac atccccaata
    19381 gtggcaggaa agcacagatt ttatagacta actcgatgct ggtgtgatcg gcgataagcc
    19441 ccagaactgc cgctcccaga cctcccatgc caaaagcaaa accgaaaaag agtccagaaa
    19501 ccataccgat acgtcccgga agtagctcct gagcgtagac cagaatggca gagaatgccg
    19561 aagcgaggat aaatccaata atcaccgtta aaacccccgt ccagtgcagg ctggcgtagg
    19621 gtaaaatcag cgtaaacggc gcaacgccga ggatagagcc ccaaatcaca tatttccgtc
    19681 caattttatc ccctacaggc ccgccgatca ccgtacctgc cgcaacggca aacaggaagg
    19741 caaacagatg aagctgggca ttctggatag ataatccgaa tttttgcatc agataaaagg
    19801 tgtaatagct gctgatgctc gccatataga aatatttcga gaaaatgagg attaacagaa
    19861 tgctgactgc cagtaccact ttgttgcgcg gcagtggatt gattatcgtc gctttgggtt
    19921 ttcctttatt cattcggtgc tgtgccgagt accaacggct gatttgtgcc aacaccacga
    19981 tcgccagcag tgccgcaagc acaaaccagg caacgttgcc tttgccataa ggcgcgataa
    20041 tcaccgccgc cagcaagggt cccagggaac tgccaaagtt gccgccgacc tgaaagatag
    20101 attgtgccag gccatgccgc ccgccggaag ccatacgggc cacgcgagaa gattccggat
    20161 gaaagaccga tgaaccggta ccgaccagcg ccgccgccag cagaactgcg ccaaaactgc
    20221 ccgccagcgc aagcagcacc agaccactta aggtaaagca catgccaatt ggcaacgacc
    20281 acggcatcgg atatttatcg gtccagtagc cgaccactgg ttgcagtagc gaagaggcga
    20341 gctggaaggt gagggttatc atgccaatct gcataaatgt cagagaaaat tctgactgaa
    20401 gcagcggata aatcgccaga atcagcgatt ggatcatgtc gttcagcaga tgtgagaggc
    20461 tgatagcacc taaaatacca aacgatgttc gggccttggt cgttgacgca gccgcgcccg
    20521 ccgcaggctg gggttgttca ctcattgcca taggaaagtc actttttcag ggttgcgatg
    20581 taaagaatga tcttatttgt gattattacc cgactaacat acctgtatgc gtcgtctgaa
    20641 ggaagtctca acgccgaata cagaatttct aatctggatg cagatttatc ttcaccggac
    20701 gcagacttgt ctatgatgtc gcgtcatact atttttcaac acgttgaaat caggtcaggg
    20761 agagaagtat gaaattattg cagcggggcg tggcgttagc gctgttaacc acatttacac
    20821 tggcgagtga aactgctctg gcgtatgagc aggataaaac ctacaaaatt acagttctgc
    20881 ataccaatga tcatcatggg catttttggc gcaatgaata tggcgaatat ggtctggcgg
    20941 cgcaaaaaac gctggtggat ggtatccgca aagaggttgc ggctgaaggc ggtagcgtgc
    21001 tgctactttc cggtggcgac attaacactg gcgtgcccga gtctgactta caggatgccg
    21061 aacctgattt tcgcggtatg aatctggtgg gctatgacgc gatggcgatc ggtaatcatg
    21121 aatttgataa tccgctcacc gtattacgcc agcaggaaaa gtgggccaag ttcccgttgc
    21181 tttccgcgaa tatctaccag aaaagtactg gcgagcgcct gtttaaaccg tgggcgctgt
    21241 ttaagcgtca ggatctgaaa attgccgtta ttgggctgac aaccgatgac acagcaaaaa
    21301 ttggtaaccc ggaatacttc actgatatcg aatttcgtaa gcccgccgat gaagcgaagc
    21361 tggtgattca ggagctgcaa cagacagaaa agccagacat tattatcgcg gcgacccata
    21421 tggggcatta cgataatggt gagcacggct ctaacgcacc gggcgatgtg gagatggcac
    21481 gcgcgctgcc tgccggatcg ctggcgatga tcgtcggtgg tcactcgcaa gatccggtct
    21541 gcatggcggc agaaaacaaa aaacaggtcg attacgtgcc gggtacgcca tgcaaaccag
    21601 atcaacaaaa cggcatctgg attgtgcagg cgcatgagtg gggcaaatac gtgggacggg
    21661 ctgattttga gtttcgtaat ggcgaaatga aaatggttaa ctaccagctg attccggtga
    21721 acctgaagaa gaaagtgacc tgggaagacg ggaaaagcga gcgcgtgctt tacactcctg
    21781 aaatcgctga aaaccagcaa atgatctcgc tgttatcacc gttccagaac aaaggcaaag
    21841 cgcagctgga agtgaaaata ggcgaaacca atggtcgtct ggaaggcgat cgtgacaaag
    21901 tgtgttttgt acagaccaat atggggcggt tgattctggc agcccaaatg gatcgcactg
    21961 gtgccgactt tgcggtgatg agcggaggcg gaattcgtga ttctatcgaa gcaggcgata
    22021 tcagctataa aaacgtgctg aaagtgcagc cattcggcaa tgtggtggtg tatgccgaca
    22081 tgaccggtaa agaggtgatt gattacctga ccgccgtcgc gcagatgaag ccagattcag
    22141 gtgcctaccc gcaatttgcc aacgttagct ttgtggcgaa agacggcaaa ctgaacgacc
    22201 ttaaaatcaa aggcgaaccg gtcgatccgg cgaaaactta ccgtatggcg acattaaact
    22261 tcaatgccac cggcggtgat ggatatccgc gccttgataa caaaccgggc tatgtgaata
    22321 ccggctttat tgatgccgaa gtgctgaaag cgtatatcca gaaaagctcg ccgctggatg
    22381 tgagtgttta tgaaccgaaa ggtgaggtga gctggcagta atccgaaagt gccggatgtt
    22441 tgcatccggc acaatgctta atcgcggcgg gcgatatcag caaatttggc atcgaggatc
    22501 tttgccagat cgcctgccgc cagttcgata tccagtccgc gcttgccgcc ggaaacataa
    22561 atagtggcaa attcttgtgc gggggcgtcg ataatcgttg gcagacgttt tttctgcccc
    22621 agtgggctaa tccccccaac caggtatccc gtcgaacgct gcgcgaccat cggatcggcc
    22681 atctcaactt tcttggcacc cagcgctttt gctacttttt taagatccag ttgacctgcg
    22741 accggcgtaa cggccacggc aaggtgtttc atatcaccgt tcactgccac cagcagcgtt
    22801 ttgtagacct gatccggatt caaacctaat tttttgacga cttcatcgcc aaaattggtt
    22861 tcagccggat cgtgctcgta ggtatggatt tgaaacgaaa tcttgttttt ttcgagtaat
    22921 ttaactgcgg gtgtcatggt aattttcctg ccttaaacaa aaaaacgaag caagcatacg
    22981 cctgatggcg gtccaaaaaa aagagtcatc ttgcctaaga gtattggcag gatggtgaga
    23041 ttgagcgaca atcgagttac accgtcgctt aaagtgacgg cataataata aaaaaatgaa
    23101 attcctcttt gacgggccaa tagcgatatt ggccattttt ttagcgcaac atttgcggca
    23161 aattcccttc tccatacagg tgtagtgcac cgaccgcgac cacatatcgc cccggcggca
    23221 tggcgcgtaa tttatcccgc caggcgagat ttcgctgatg catcagcaca tcgtacagcg
    23281 actgactgaa cgtattgggc agcgttatat cattattttg cggaggtgca ttcagccacc
    23341 agctcatcat ttgttgcagc aaccgtgcgt tggtatgcca gtgggtcagc gtatcgtcca
    23401 gcagcgccag tcctttgtca gggagctgga gcaacatggt aatctggttt tcagcccctt
    23461 ccagttcaat cacgggttta tgttgttgct tcgccgcctg caataactgg taatcgatac
    23521 cgtattctgc ccgcagcccc agtttttgtg cctgcgtcgc ctgaagaacc atcgcgattt
    23581 gccacagcgg ttgagtagaa aagagcgacg gagaaatgcc catctcctgg ctaatgtgct
    23641 gcagattttg gagttgctcc tcgttaatgc gctcttccag cgcctcgcaa gtaggcaaat
    23701 tagcaaaagg cgtatcgctg gtggaaacat ccgcctcgac gatcagcgca tcggcgtttt
    23761 tgagcttttt gagcaaacgg gtgggcaggg gagccatatc gtggctaccc atatgaatac
    23821 tgccaatcag atgaaaatgg cgattgccgg gaagggtgat atcgatggca ggccaggtgt
    23881 aatgattacc gcgcagcgcg gcccaaagcg tttttacccg gtacaacaga tccatacgac
    23941 ctccctttgt gaaatatcat gctagcgcgc ggggagggat ggcgcaaatg gagttgggga
    24001 cttgtttaat gtgtttgtat gattcagtat gttcttgtat cgctattcac agggaagcaa
    24061 cagttaaaaa ctatgaaaca ggcaacaaga aaaccgacga cacccggaga tattcttctc
    24121 tatgaatatc tggaaccgct cgatttgaaa atcaatgagt tagcagagtt gctgcatgtt
    24181 catcgtaata gcgtcagtgc actgatcaat aacaatcgta aactcactac tgagatggca
    24241 tttcgtctgg cgaaagtttt tgataccaca gtcgattttt ggctaaacct ccaggcggcg
    24301 gttgatcttt gggaagttga aaacaacatg cgcacccagg aagaattggg acggattgaa
    24361 acagtggctg aatatttagc acgccgtgaa gagcgtacaa aaaaggtcgc gtaaggccaa
    24421 aacatcgctt catctcggat gccggataag gcgtt
//
LOCUS       DACAPB010000053        24310 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.053, whole genome shotgun sequence.
ACCESSION   DACAPB010000053 DACAPB010000000
VERSION     DACAPB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.053"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..328)
                     /locus_tag="IT904_001927"
     CDS             complement(<1..328)
                     /locus_tag="IT904_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="with EfeUB forms an iron transport system which is
                     silent in E. coli K-12 due to a mutation in EfeU; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter"
                     /protein_id="HAP3191714.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNP"
     gene            complement(386..1216)
                     /locus_tag="IT904_001928"
     CDS             complement(386..1216)
                     /locus_tag="IT904_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HAP3191715.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(1758..3266)
                     /gene="putP"
                     /locus_tag="IT904_001929"
     CDS             complement(1758..3266)
                     /gene="putP"
                     /locus_tag="IT904_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAP3191716.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFSSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(3425..3634)
                     /locus_tag="IT904_001930"
     CDS             complement(3425..3634)
                     /locus_tag="IT904_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191717.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3689..7651
                     /gene="putA"
                     /locus_tag="IT904_001931"
     CDS             3689..7651
                     /gene="putA"
                     /locus_tag="IT904_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP3191718.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKVNIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7691..8329)
                     /gene="rutR"
                     /locus_tag="IT904_001932"
     CDS             complement(7691..8329)
                     /gene="rutR"
                     /locus_tag="IT904_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAP3191719.1"
                     /translation="MTQGAVKTTGKRSRVVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8618..9709
                     /gene="rutA"
                     /locus_tag="IT904_001933"
                     /pseudo
     CDS             8618..9709
                     /gene="rutA"
                     /locus_tag="IT904_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
     gene            9709..10401
                     /gene="rutB"
                     /locus_tag="IT904_001934"
     CDS             9709..10401
                     /gene="rutB"
                     /locus_tag="IT904_001934"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAP3191720.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10413..10799
                     /gene="rutC"
                     /locus_tag="IT904_001935"
     CDS             10413..10799
                     /gene="rutC"
                     /locus_tag="IT904_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAP3191721.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10807..11607
                     /gene="rutD"
                     /locus_tag="IT904_001936"
     CDS             10807..11607
                     /gene="rutD"
                     /locus_tag="IT904_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAP3191722.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQVLVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11617..12207
                     /locus_tag="IT904_001937"
     CDS             11617..12207
                     /locus_tag="IT904_001937"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAP3191723.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12218..12712
                     /gene="rutF"
                     /locus_tag="IT904_001938"
     CDS             12218..12712
                     /gene="rutF"
                     /locus_tag="IT904_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAP3191724.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12733..14061
                     /gene="rutG"
                     /locus_tag="IT904_001939"
     CDS             12733..14061
                     /gene="rutG"
                     /locus_tag="IT904_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAP3191725.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMTVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(14319..14492)
                     /locus_tag="IT904_001940"
     CDS             complement(14319..14492)
                     /locus_tag="IT904_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAP3191726.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14865..15461
                     /gene="wrbA"
                     /locus_tag="IT904_001941"
     CDS             14865..15461
                     /gene="wrbA"
                     /locus_tag="IT904_001941"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP3191727.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15482..15709
                     /locus_tag="IT904_001942"
     CDS             15482..15709
                     /locus_tag="IT904_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191728.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15929..17170)
                     /gene="agp"
                     /locus_tag="IT904_001943"
     CDS             complement(15929..17170)
                     /gene="agp"
                     /locus_tag="IT904_001943"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAP3191729.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(17463..18720)
                     /locus_tag="IT904_001944"
                     /pseudo
     CDS             complement(17463..18720)
                     /locus_tag="IT904_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            18981..19901
                     /gene="cbpA"
                     /locus_tag="IT904_001945"
     CDS             18981..19901
                     /gene="cbpA"
                     /locus_tag="IT904_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAP3191730.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            19901..20206
                     /gene="cbpM"
                     /locus_tag="IT904_001946"
     CDS             19901..20206
                     /gene="cbpM"
                     /locus_tag="IT904_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAP3191731.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(20358..20957)
                     /gene="torD"
                     /locus_tag="IT904_001947"
     CDS             complement(20358..20957)
                     /gene="torD"
                     /locus_tag="IT904_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAP3191732.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(20954..23500)
                     /gene="torA"
                     /locus_tag="IT904_001948"
     CDS             complement(20954..23500)
                     /gene="torA"
                     /locus_tag="IT904_001948"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAP3191733.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGLRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(23500..>24310)
                     /gene="torC"
                     /locus_tag="IT904_001949"
     CDS             complement(23500..>24310)
                     /gene="torC"
                     /locus_tag="IT904_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAP3191734.1"
                     /translation="LAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQMKVAAK
                     DNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAKGDKEA
                     SGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGDVQQQV
                     KTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHGAPEIA
                     HFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
BASE COUNT         5771 a         6502 c         6198 g         5839 t
ORIGIN      
        1 tcgggttagt cagcagaccg caggtcatat cgtattcgcc aggctgtaaa ttcgccgtca
       61 ttttctggct aaagccaggg gcgatatttt cccgctcttc caccaccatc acgcctttga
      121 ggatctccca ctccagcgcc ttctggctgt ggttctgaat aatgaactgt gttttcccgg
      181 cgttaaccgt aatggtcatc ggttcgcact gcttatccgt cacggtcact ttgacctgcg
      241 gcacatcagc ggcgttagcc ataaaagcag aagaaaacag cgcagccacg ctcaactgca
      301 atgcgttacg gcggaagtta atggtcatga cgagactatc cctttaaagt atgttgtaac
      361 taagcaagag tttgcgtcgt atttgttacg cggagcgaga cgctgtcgcc cctgcgcgtg
      421 gtggcagagc aaatgccacc agcgccggga tgagataaat aaaccagacg gcgacttcgc
      481 tgacgctcgg cgcttcctga tagccaaaaa tcccttccat cagcgtgcca aacagcgagt
      541 gagttgagag caccgcactc atatcgaagg cgatttcctg aaagtggttc cacaatccgg
      601 cttcatgaaa tgcgcgaatg gcaccagctg ccagccctgc ggcgacgaag agaataaaca
      661 ggctggtcca tttaaaaaat gcaccaagat tgaggcgaat accgccccag tagagcagga
      721 agcctagcac cacggcagta gcaagaccga gcattgcacc cagcggcggc cagatcccga
      781 catcttgttg aaatgccgcc agcaggaaaa agaccgactc cagcccttcc cttgcaacgg
      841 caaaaaagac catcatcacc agcgcccagc catgatgatt tccacgctgc aatgcgctat
      901 cgactgcctg ttccagttgc actttgacgt tgcgcgacac tttgcgcatc cagaaaacca
      961 tccaggtaag gatcaccacg gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt
     1021 tttgcggaaa ttcgccggtg gtttcgttaa tgaagatacc caagcccagg cacaacgcag
     1081 cggcaagcaa cacgccaatc cacatcacac caatccatcg gcctcgctgg gtacgcttaa
     1141 gatagctggc aatcaaactg acaatcagcg cggcttcaag tccttcgcgc aacataatga
     1201 gaaacggaac aaacatgcca cccaccctta aacgtttttc tgagtcaatc tatgcaaaga
     1261 aaagtaaatc gcagtgatag cgattatcat tacggtaaag aaaaatacaa gcggaatgtt
     1321 tataaaatga tgttgaaatg taaacaaaga gaggtaatgc caggggatat aaacgattat
     1381 tccaggtaca aagatgggat tgtttacaac gagccatgtg ttcttgccgg atgccgcgta
     1441 aacgccgtat ccagccgaca aaactgtcca gactcaatgc atcagagctt ccatgtaaaa
     1501 ctgataagcg ggtgtatcaa gcaatttcgt ccatccgcga ttatcaaatc taactttttg
     1561 cccggaacat aataacagct cgggtgttgg tggcgtcaaa atcgctgaag cgttccccga
     1621 aggccgggac agcagggggc ggcggcgatt ggccgccccc tgcgcgctcc ctgcaccatt
     1681 caatttatat ggcgaggcac attaagggag cgcaacccgc tgctgaagcc aaacaaaacc
     1741 gccgcaggct aagtccctta gctttcctgc aaccgtgacg gcggagccga atgatagtgc
     1801 gcatcggcct cggcaaagcg tttttgcatc gccgctgacg gcgctttacc cagcaaacta
     1861 aacactacaa tcccaatact gctgaagata aagcccggaa taatttcgta cagtcccagc
     1921 cagccgaact gtttccagac gataaccgtc agcgccccga tgatcatccc cgccagcgca
     1981 ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca ccaccggacc aaacgccgcg
     2041 ccaaagcctg cccacgcata gctcactagc cccagcacac ggttttccgg gtttgccgcc
     2101 agcgcaatgg ccaccagcgc caccaccagc accatcacac gacctaccca caccagctct
     2161 ttctggctgg catgtttacg cagaaacgct ttgtataaat cttcggtaat cgcactggag
     2221 cacaccagca gctggcaact taaggttgac attaccgccg ccagaattgc cgacagcaga
     2281 atcccggcaa tccacgggtt aaacagaatt tgcgccagtt cgataaacac gcgctcagca
     2341 ttctggttta ccgcacctgc taccgccggg tgctcattaa agtaagcaat accaaagaag
     2401 ccgacagcta ccgcccctgc caggcagagg atcatccagg tcatactgat acgacgcgca
     2461 tggacaatgc tgtggtgaga gtctgccgcc ataaaacgcg ccaggatgtg cggttgcccg
     2521 aagtaaccca gcccccaacc catcagtgag ataatggcga caaagttcag ccctttgagc
     2581 atatcaacgt tttcgatgct cttttgtttg atcacttcca gcgagtcacc aaagccaccg
     2641 acactgataa tgacgataac cggcgtcagg atcagggcaa aaatcatcag gctggcctgt
     2701 acagtgtcag tccagctcac cgcgaggaaa ccgccaataa aggtgtaaag gatcgtcgcc
     2761 gcagctcccg cccacagagc cgtttcgtag ctcatgccaa aggtactttc aaacagacgc
     2821 gcgcctgcca caatgcccga agcgcaataa atggtgaaga acagcaaaat caccagcgcg
     2881 gagataatgc gcaaaatgcg gcttttatct tcaaagcgcc cggtgaaata atccggcaat
     2941 gttaaggcgt tattgttgta ttcggtatgc acacgcaacc gccccgccac cagcttccag
     3001 ttgatccacg cgcctaatgt caggccaatg gcgatccaac tttcggaaat cccggaaaga
     3061 aaaaccgcgc ccggcaaccc cattaacagc cagccgctca tatccgacgc acccgccgat
     3121 aatgccgtca cgaatggccc aaggctacga ccgcccagaa tatagtcgtc aaagtttttc
     3181 gttgatcgcc aggcgataaa cccaatcaat atcatgccaa agatatagac acaaaatgtc
     3241 accaacatcg gtgtgctaat agccatctaa agtctccaaa aaattattat cggcaatgtc
     3301 gaaacttgcc gttatatctg ccaccggaac ggggtaacag agtttatgtt ttaccagggc
     3361 gaccgtatcc tgccggaagc gctggttatt cacaatcgat ttaacacacc atttacatta
     3421 aattttagtg ctcagcgaca ctatttttca tcaggttgca ctctctcaca ttttttgcgg
     3481 ttgcaccttt caaaaatgtt aactgccgca gagaaaaagt ctgagttatt tttttaatcc
     3541 ctgtcatatc gatttctttt attaacattt cattcatttt taagcttgct acgcatgtca
     3601 catttaacat ggttgcacaa agttgcaaca tcatggatat ttcacgataa cgttaagttg
     3661 cacctttcag aacaacagga gtaatggcat gggaaccacc accatggggg ttaagctgga
     3721 cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt atcgatcgca cgccacactg
     3781 gttaattaag caggcgattt tttcttatct cgaacaactg gaaaacagcg atactctgcc
     3841 ggagctacct gcgctgcttt ctggcgctgc caatgagagc gatgaagcac cgactccggc
     3901 agaggaacca caccaaccat tcctcgactt tgccgagcaa attttgcccc agtcggtttc
     3961 ccgcgccgcg atcacagcgg cctatcgccg cccggaaacc gaagcggttt ctatgctgct
     4021 ggaacaagcc cgcctgccgc agccagttgc tgaacaggcg cacaaactgg cgtatcaact
     4081 ggccgataaa ctgcgtaatc aaaaaaacgc cagtggtcgc gcaggtatgg ttcaggggtt
     4141 attgcaggag ttttcgttgt catcgcagga aggcgtggcg ctgatgtgtc tggcggaagc
     4201 gttgttgcgt attcccgaca aagccacccg tgatgcgtta attcgcgaca aaatcagcaa
     4261 cggtaactgg caatcacaca ttggtcgtag cccgtctttg tttgttaatg ccgccacctg
     4321 ggggctgctg tttactggca aactggtttc cacccataac gaagccagcc tctcccgctc
     4381 gctgaaccgc attatcggta aaagcggtga accgctgatc cgcaaaggtg tggatatggc
     4441 gatgcgcctg atgggcgagc agttcgtcac tggcgaaacc atcgcggaag cgttagccaa
     4501 tgcccgcaag ctggaagaga aaggtttccg ttactcttac gatatgcttg gcgaagctgc
     4561 actgactgcc gcagatgcac aagcgtatat ggtttcctat cagcaggcga ttcacgccat
     4621 tggtaaagcg tccaatggtc gtggcatcta tgaaggtccg ggcatttcaa tcaagctgtc
     4681 ggcgctgcat ccgcgttaca gccgcgccca gtatgaccgg gtaatggaag agctttaccc
     4741 acgtctgaaa tcactcaccc tgctggcgcg tcagtacgat attggtatca acattgacgc
     4801 cgaagaggcc gatcgtctgg agatctccct cgatctgctg gaaaaactct gtttcgagcc
     4861 tgagctggca ggctggaacg gtatcggttt tgttattcag gcttatcaaa aacgctgccc
     4921 actggtgatt gattacctga ttgatctcgc cacccgcagc cgtcgccgtc tgatgattcg
     4981 cctggtgaaa ggcgcgtact gggatagtga aatcaagcgt gcgcagatgg acggccttga
     5041 aggctatccg gtttataccc gcaaggtgta taccgacgtt tcatatctcg cctgtgcgaa
     5101 aaagctgctg gcggtgccga atctaatcta cccgcagttc gcaacgcaca acgcccatac
     5161 gctggcagcg atttatcaac tggctggtca aaactactat ccgggtcagt acgagttcca
     5221 gtgcctgcat ggtatgggcg agccactgta tgagcaggtc accgggaaag ttgccgacgg
     5281 caaacttaac cgtccgtgtc gtatttatgc cccggttggc acacatgaaa cgctgctggc
     5341 gtatctggtg cgccgcctgc tggaaaacgg tgctaacacc tcgtttgtta accgtattgc
     5401 cgacacctct ttgccactgg atgaactggt cgccgatccg gtcactgctg tagaaaaact
     5461 ggcgcaacag gaagggcaaa ctggattacc gcatccgaaa attcccctgc cgcgcgatct
     5521 ttacggtcac gggcgcgaca actcggcagg gctggatctc gctaacgaac accgtctggc
     5581 ctcgctctct tctgccctgc tcaatagtgc actgcaaaaa tggcaggcct tgccaatgct
     5641 ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt aaccctgcgg aaccgaaaga
     5701 tattgtgggc tttgtgcgtg aagccacgcc gcgtgaggta gaacaggcgc tggaaagtgc
     5761 ggttaataac gcgccaatct ggtttgccac tcctccggtt gaacgcgcgg cgattttgca
     5821 tcgcgctgcc gtgctaatgg aaagccagat gcagcaactg attggtattc tggtgcgtga
     5881 agccggaaaa accttcagta acgccattgc cgaagtgcgc gaagcggtcg attttctcca
     5941 ctactatgcc ggacaggtgc gggatgattt cgctaacgaa acccaccgtc cattagggcc
     6001 tgtggtgtgt atcagtccgt ggaacttccc gctggctatt ttcaccgggc agattgctgc
     6061 cgcactggcg gcaggtaaca gcgtgctggc aaaaccggca gaacaaacgc cgctgattgc
     6121 cgcgcaaggg atcgccattt tgctggaagc gggtgtaccg ccaggcgtgg tgcagttgct
     6181 gccaggtcag ggggaaaccg tgggcgcgca actgacgggt gatgatcgcg tacgcggggt
     6241 gatgtttacc ggttcaaccg aagtcgctac gttactgcag cgcaatatcg ccagccgcct
     6301 ggacgctcag ggtcgcccta ttccgctcat cgctgaaacc ggcggcatga acgcgatgat
     6361 tgtcgattct tcagcactga ccgaacaagt agtaatagat gtgctggcct cggcgttcga
     6421 cagtgcgggt cagcgttgtt cggcgctgcg cgtgctgtgc ctgcaagatg agattgccga
     6481 ccacacgttg aaaatgctgc gcggcgcaat ggccgaatgc cggatgggta atccgggtcg
     6541 cctgaccacc gatatcggtc cggtgattga tagcgaagcg aaagtcaata ttgagcgcca
     6601 tattcagacc atgcgtagca aaggccgtcc ggtgttccag gcggtgcggg aaaacagtga
     6661 agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg acgctgatcg aactggatga
     6721 ctttgccgaa ttgcaaaaag aggtctttgg tccggtgctg catgtggtgc gttacaaccg
     6781 taaccagcta ccagagctga tcgagcagat taacgcttcc ggttatggtc tgacgcttgg
     6841 cgtccatacg cgtattgatg aaactatcgc ccaggtcact ggctcggccc atgttggtaa
     6901 cctgtatgtt aaccgtaata tggtgggcgc agtggttggt gtgcagccgt tcggcggcga
     6961 agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc tatctctacc gtctgctggc
     7021 gaatcgcccg gaaagtgcgc tggcagtaac gctcgcgcgt caggatgcag agtatccggt
     7081 cgatgcgcag ttgaaagccg cattgactca gccgctaaat gcactgcggg aatgggcagc
     7141 aaatcgtcca gaattgcagg cgttatgtac gcaatatggc gagctggcgc aggcaggaac
     7201 acaacgattg ctgccggggc cgacgggtga acgcaacacc tggacgctgc tgccgcgtga
     7261 gcgcgtgttg tgtattgccg atgatgagca ggatgcgctg actcagctcg ccgccgtgct
     7321 ggcggtgggc agccaggtac tgtggccgga tgacgcgctg catcgtcagt tagtgaaggc
     7381 attgccatcg gcagtcagcg aacgtattca actggcgaaa gcggaaaata taaccgctca
     7441 accgtttgat gcggtgatct tccacggtga ttcggatcag cttcgcgcat tgtgtgaagc
     7501 cgttgctgcg cgggatggcg caattgtttc ggtgcagggt tttgcccgtg gcgaaagcaa
     7561 tatccttctg gaacggctgt atatcgagcg ttcgctgagt gtgaataccg ctgccgctgg
     7621 cggtaacgcc agcttaatga ctataggtta aacaggtagc cggaggatgt tacaacctcc
     7681 tccggcatct ttaacgtggt cgaatcccct caataataat ccgctgcacg ttttcaaccg
     7741 tttgattgaa aaatacctca tcgcgcaacg tcgcgcctgt caccgcctcc acctgagggg
     7801 cgaaatcggc gtaatgttga gtggaagccc aaatcataaa aatcaaatgc tgcggatcaa
     7861 tcggcgcgag tttgccgctt ttgacccaac cggcaatcag cgccgatttc tcatcaatta
     7921 atgccttcaa atcgcccgtc agttcatcca ttaacagcgg cgcgcctgcc agcatctcca
     7981 tacagaacag gcgcgaagcc tgcggataat cgcgtgagac ttccagcttc agacggatgt
     8041 actctttgat cgccgccagc ggggcgaaat cttctcgaaa cgcttttaac ggtgccagcc
     8101 agatatcgag aatctgccgc agcacggcaa tatacagcgc ctcttttgac ggaaagtaat
     8161 acagcagatt ggtttttgaa acacccgcca actctgcgat ctgctccagc cttgtgccgt
     8221 gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag aatcgctttt ttcttcgcgc
     8281 ttactacgcg cgaacgttta cccgttgttt tcactgcgcc ttgcgtcatg cgctctcccc
     8341 tcttgtagat tggcaacagg atagcaaaac ccccgcccgg cctcgacctt ctgcactctc
     8401 atcgcgcacg gatgcatgtt ttatgtgcaa ctgttttgac cgtttagtcc gctttttacc
     8461 agatatttta gataacttaa cattaacaca ttaataacat tcactttttt aaaaactggc
     8521 atccactttg caaacaagcc attacgcggc tgaaaaggat atgcaggacg cagtgccccg
     8581 tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg tattcgtacc
     8641 tattggcaac aacggctgac tcatttcgac ccacgcgccg cagtacatgc cgacctttga
     8701 actgaataaa gccatcgtgc aaaaagcgga gcactaccat ttcgatttcg ccctgtcgat
     8761 gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc ttgagtcgtt
     8821 caccttgatg gcggggctgg cggccgtgac ctcgcgcatt cagatttacg ccacagccgc
     8881 caccttaacg ttacctccag caatcgtcgc ccgtatggcc gcaaccatcg actcaatctc
     8941 tggcgggcgt tttggcgtca acctcgtgac tggctggcaa aagcccgagt atgagcagat
     9001 gggtatctgg cctggcgatg actatttctc ccgtcgttac gactatctca ccgagtatgt
     9061 tcaggtgctg cgcgacctgt ggggcacggg aaaaagcgat tttaaaggcg attttttcac
     9121 catgaatgat tgtcgcgtca gtccgcaacc gagtgtccct atgaaagtga tctgcgccgg
     9181 gcaaagcgac gctggcatgg cgttctccgc ccagtatgcc gatttcaact tctgtttcgg
     9241 caaaggcgta aatacaccca cggctttcgc cccgaccgct gcgcggatga aacaggccgc
     9301 agagcaaacc gggcgcgacg ttggctctta tgtattgttt atggtgattg ccgatgaaac
     9361 cgacgatgcc gctcgcgcca aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt
     9421 aagctggcta accgaacaaa gtcagaaaga tacccgctca ggtactgaca ccaacgtccg
     9481 tcagatggcc gatcccactt cggcggtaaa catcaatatg gggacgttag tcggttctta
     9541 cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg aaggcgtgct
     9601 gttaaccttc gacgattttc tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact
     9661 gatgcagtgc cgcgcccatc tccctgtgct gactcaggag gtggcatgat gacgacatta
     9721 accgctcgac cggaagccat taccttcgat ccgcagcaaa gtgcgctgat cgtggtggat
     9781 atgcaaaacg cctatgccac gccaggcggc tacttagatc tcgccgggtt tgatgtctca
     9841 accactcgcc cggtcattgc caacattcaa accgccgtga ctgcagcgcg agcggcaggg
     9901 atgttgatca tctggtttca aaatggctgg gatgaacagt atgtcgaggc tggcggccct
     9961 ggctcaccga attttcataa atcgaacgcc ctgaaaacca tgcgtaaaca gccgcagctg
    10021 caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga actggtgccg
    10081 cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa tacgccgctg
    10141 gacagcattt tgcgcagccg cggaatacgc catctggttt tcaccggcat cgctaccaac
    10201 gtctgcgtcg aatcgacgct acgcgacggc ttttttctgg agtatttcgg cgtggtgctg
    10261 gaagacgcaa ctcaccaggc ggggccggaa tttgcgcaga aagctgcgtt gttcaatatc
    10321 gaaacctttt ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct ttctcccacg
    10381 tcctttgctc gtatcgctta aggagtttaa cgatgccaaa atccgtaatt attcccgctg
    10441 gcagcagcgc accgctggcc cccttcgttc ccggcacgct ggctgatgga gtggtgtatg
    10501 tctccggtac gctggctttt gatcaacata ataacgtgct gtttgccgat gacccaaagg
    10561 cgcaaacccg ccacgttctg gaaaccatcc gcaaggtgat cgagacggcg ggtggcacga
    10621 tggcggatgt gaccttcaac agcatcttta ttaccgactg gaaaaattac gccgcgatta
    10681 acgaaatcta cgccgagttt tttccgggtg ataaaccggc gcgattctgc attcagtgcg
    10741 gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag
    10801 gccgcgatga aactttcact ctcacctccc ccttatgctg atgcacccgt agtggtgttg
    10861 atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt gctggagcag
    10921 gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga cacactggca
    10981 gaagattaca gtatcgccca gatggcagcg gaactgcatc aggtgctggt agccgcaggg
    11041 attgagcatt acgcggtggt cggccatgcg ctcggtgcgc tggtgggaat gcagctggcg
    11101 ctggattatc ccgcgtcggt aactgtgctg gtcagcgtta acggctggct acgaataaac
    11161 gcccatacgc gccgctgttt tcaggttcgc gaacgattac tgtatagcgg tggcgcgcag
    11221 gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc ggcccgcgca
    11281 cctcgccttg aggcagaaga cgcgctggca ctggcgcatt ttcagggcaa aaataattta
    11341 ctgcgtcgac ttaacgccct caaacgcgct gactttagtc accatgcgga tcgcatccgc
    11401 tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccatcagc atgttccagt
    11461 gaacttcatg ccgccctgcc cgatagccag aaaatggtga tgcgctatgg cggacacgcc
    11521 tgcaacgtga ccgatcccga aacgtttaat gctctgttac tcaacgggct tgccagcctg
    11581 ttacatcacc gtgaagccgc cctgtaagga attgctatga acgaagccgt tagcccaggt
    11641 gcgcttagca ccctgttcac cgatgcccgc actcacaatg gctggcggga gacacccgtc
    11701 agcgatgaga cgttacggga gctttatgcc ctgatgaaat gggggccgac atcagctaac
    11761 tgttctccgg cacgtatcgt gtttatccgc actgcagagg gaaaagaacg tctgcgcccg
    11821 gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc tatcgtcgca
    11881 tgggacagtg aattttatga acggttaccg caactgtttc ctcacggcga tgcccgcagt
    11941 tggtttacct ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag ttccatgcag
    12001 gcggcctatc ttatcatcgc ttgccgtgcg ctgggactgg ataccggccc gatgtcgggt
    12061 tttgaccgtc aacacgttga cgacgccttt tttgcgggca gcacgctgaa gagcaatctg
    12121 ctgattaata tcggctatgg cgatagcagc aaactttttg cgcgcctgcc acgtctgtcc
    12181 tttgaagaag cctgcgggct gttgtaagga gcaaactatg aacattgtcg atcaacaaac
    12241 ttttcgcgat gcgatgtcct gcatgggcgc ggcggtcaat atcatcacca cggacggtcc
    12301 agccgggcgc gccgggttca ccgccagcgc cgtctgcagc gtgaccgaca cgccgcccac
    12361 attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg aaaatcgaac
    12421 gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt ttggcggcaa
    12481 aacgcccatg gaacaccgct ttgccgccgc ccgctggcag actggtgtga ccggatgccc
    12541 gcaactggaa gaggcgctgg tttcgtttga ctgccgtatc agccaggtgg tgagcgtcgg
    12601 cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacgcca caccctacgg
    12661 gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt aacctcctta
    12721 aggagacagc tgatggcaat gttcggtctt cctcactggc agttaaaatc gacctctaca
    12781 gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcagacggc aataatgggc
    12841 gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccgattct gatggggctg
    12901 gatcccaatc tttccatttt aatgtcgggg atcggcacat tactgttttt ctttattacc
    12961 ggcgggcgcg tccccagtta tctcggttcc agcgcggctt ttgtcggcgt ggtgattgcc
    13021 gcgacgggct ttaacggtca gggcattaac ccgaatatca gcattgcgct gggcgggatt
    13081 atcgcctgtg ggctggttta taccgtcatt ggcctggtgg tgatgaaaat cggtacacgc
    13141 tggattgaac ggctaatgcc gccggtggtg acgggtgcgg tggtgatggc gattggcctg
    13201 aacttagcgc ctatcgcagt gaaaagtgta tcggcttcgg cctttgatag ctggatgacg
    13261 gtaatgacgg tgctgtgtat tgggctggtg gcggtgttta cccgtggcat gatccagcga
    13321 ttattgattc tcgtaggatt aattgtcgcc tgtctgctgt atggcgtgat gaccaacgtt
    13381 ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg gttcggtttg
    13441 ccacattttt ccactcctgc ttttaatggt caggcaatga tgctgattgc gcccgtggcg
    13501 gtgattctgg tggcggaaaa cttaggccat ctcaaggcgg tcgccgggat gaccggacgc
    13561 aatatggatc catatatggg acgggcattc gtcggtgatg ggctggcgac gatgctttct
    13621 ggttctgtcg gtggcagcgg cgtaaccacc tacgccgaga atatcggtgt aatggccgtg
    13681 acaaaagtct actcgacgct ggtgtttgtc gctgctgccg tcatcgccat gctgttgggc
    13741 ttttcaccga aatttggcgc gctgattcat accattccgg ccgcggtgat tggtggtgca
    13801 tcaattgttg tgttcgggct gattgccgtc gcaggcgcaa ggatctgggt acaaaaccgt
    13861 gtcgatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt gctgggcgcg
    13921 ggtgattttg ctctcacgct gggcggtttt acgttgggag ggattggtac agcaaccttt
    13981 ggcgcgattt tactcaatgc gttgttaagc agaaagttgg ttgacgttcc accgccagaa
    14041 gttgttcatc aggagccata aacagaaaac cgccgggatt acccggcggt gcagattgtt
    14101 gacaacgtac gcattgttca tgccggatgc ggcgtaaacg ccttatccgg cctacaaaac
    14161 agtgcaaatt caatatattg caatcttctg gtaggcctga taagcatcgc gcatcaggca
    14221 agcttacatt tgtcatcagt atcaaccacc gggtaaccgg cggtgatggc tttttgacga
    14281 gctacagaca aaaatgcatt cgtcattgat tagcccggct agttgtcgct tttgccgtga
    14341 ctgctcttgc cacctttttt acctgcttca gatgcgcgct gcgggtcatt cttgaaatta
    14401 cccccgctgt gctgtccacc ttttttacct gcttctgatg ctctttcgcg gtcttctgca
    14461 aaattgccgg aaccgcctcg atggtttgcc attactgacc tccgtctggg tatagacatc
    14521 atattgcata gaattaacgc ggcgtatggc ggctgccata ttttcgctac gtgggactaa
    14581 gcttagtgaa ctgacgcggg cagacccgcg tttggtaata tcctgcaaca gcttcttagc
    14641 gagaatagat atcatctcca ataattatcc ataagccgct cttatgatta aaaaaacgcg
    14701 atgagataaa aatgtgtcat tttgcgacaa aattacgtgc ttgtgaaagt tgttataaat
    14761 caaataagtg gttgtgaaat ttgcactctg aaaaggacgt cttatcttta aataagtggt
    14821 agcgaatcgc tacggaatag agataacacg aggagtggtt agaaatggct aaagttctgg
    14881 tgctttatta ttccatgtac ggacatattg aaacgatggc acgcgcagtc gctgagggtg
    14941 caagcaaagt ggatggcgca gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc
    15001 aattatttga aaaagcaggc ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac
    15061 tggccgatta cgacgccatt atttttggta cacctacccg ctttggcaac atgtccggtc
    15121 aaatgcgtac cttcctcgac cagacgggcg gcctgtgggc ttccggcgca ctatacggaa
    15181 aactggcgag cgtctttagt tccaccggta ctggcggcgg tcaggaacaa actattactt
    15241 caacctggac gacccttgcg catcacggca tggtaattgt ccccattggc tacgcagcgc
    15301 aggaattatt tgacgtttca caggttcgcg gcggtacgcc gtacggcgca acaaccatcg
    15361 caggcggtga cggctcacgc cagccaagcc aggaagaact gtctattgct cgttatcaag
    15421 gggaatatgt cgcaggtctg gcagttaaac ttaacggcta atcttcaaca ggaggatacg
    15481 catgccaact caagaagcga aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac
    15541 gtcgccggaa atagccgagg ccattttcga agtcgccggg tacgacgaaa aaatggcgga
    15601 aaagatttgg gaagaaggta gcgatgaagt gttagtcaaa gcctttgcta agaccgataa
    15661 agattcgctt ttttggggcg aacagaccat cgaacgtaaa aacgtttaac cagcggctcc
    15721 cccgcttcac gcgggggggc tttcaaattg ttgacaaagt gcgctttgtt catgccggat
    15781 gcggcgtgaa cgccttatcc ggcctacatg atcgtgcaaa ttcgatatag tgcaattctc
    15841 gggtaggcct gataagcgta gcgcatcagg caatctcacg tttgtcatca atctcatact
    15901 cccccgcttc tcgcggggga gttttctgtt atttcaccgc ttcattcaac acgctatcaa
    15961 atttatccat cgggcagaaa ccgttggcat ctatcgggca accgcttaat tccagcgtca
    16021 cacgctgagc aggtgcctgc agggttaacg catcggcatt acgtaactgt tccgcactct
    16081 gatacacata ttcaattttc atcaaatcgc gattggcttt gctgtcatgc caacgctgga
    16141 aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc atgcaactga tacggtttga
    16201 aatccagcgc cgttaacaga gaggcaatgt tggagtcgtg cccaaccaac actgtaattt
    16261 tcggtgcgct ggtgcgatcg gtgaccagag ctttgtcgat ataactgacc agcggtttcg
    16321 caacattgcg cgccacttcc ggtgaggtaa acaggctgtc ctggtagccg tttttaagct
    16381 tcgacaacac cttccactgc tggtcagatt tgatttctcc ccaggccacc tgatccatcg
    16441 gaaaaccttc gtagtattgc aaagtaaacg catctaccag cgagttgccg actttcagcg
    16501 gcccggaaac acctggttct tgttgatact tcgcgctaaa ggtatttttg ccatccacca
    16561 gcgaacactg ttgtttctct ttacaggcag gggaatcttt atagttaacg attttttcca
    16621 gtagctggta gctgtcggta agctggagtt tgctgagctc tttctccatt gccgccaccg
    16681 cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg gttaaaggtt gggtccatag
    16741 tgcccatttt ttcctggtga tgcacaggaa tatcacaccc cgggaatgcg ccggtaataa
    16801 agaactgtgc ggtcgcaacg gtacgttgca gactattggc ataggcataa acggtgtacg
    16861 gcggcgggca ttcccccgat ttcaccattc cctgctctgc cagccattca cgcatgtaat
    16921 ggcccatata cacttcgagc acgccacctt tggtggtgag ttgcccaccg gggacgtccc
    16981 attctggcca tttattcggc gtcgactgct ccagcacact gccattgttc gccagcggcg
    17041 cacgtaagtt atggcggctc atcatgagca cttgctgtag ctgatagcct tccggtacgg
    17101 tttgtgcctg agcgtttgaa gcgagtaaaa ctatccctgc cacagctgcg gcgattagcg
    17161 ttttgttcat tcctggcacc tcttttgtta tcaatcacta aagagtgtga cagaaatatg
    17221 acatttttcc gggaacaggt tcgcaaaatg gcttgttgaa cgcctgtttt tctatataca
    17281 tcttcaaaaa caggcaaggt ttgacgcaaa ctatgcctga ttagcccctg cctcgcgtac
    17341 gccgctgata attgcacctg tggctaaaca gaatatctgc ggcgttacca atacagagac
    17401 aatatttacc aataaaaaag tgtgagacta ataacaatgg tttttagcat atgcctttgc
    17461 cattaaattg cataacgatt taaatagtcg agttgcgcat tcatttcctt attgtttaac
    17521 ttgtaagcct gagcaaaaag ttcaggaggg aggtaaacgt cataatgatt gccagtgttc
    17581 attaaataac catactcaag atcttcatct gttgaaaagg attcattccc caaggagatt
    17641 gatgccacat tactattaac aataaataat gagcaaatgc tatctgaaag aacagcaaga
    17701 aaattcttca ccgctttaat cctatccgac gtgtcggtat ctaaagcaag caagttcttg
    17761 ttatcattaa atataaactc gggtaaatgc aggatacatt tttcgttatt caagcaacta
    17821 tcaatgaatt gttttttaaa aaacagaaag ctccatgaca taagactact ctgcccctga
    17881 atttggttgt cagattgtag atcaacgcca aataacgtct ttacatcttc cctgagatta
    17941 tagaatgccg tatcaacaag agcctcgatc gtttttttat caaccatttt aatgaattcg
    18001 ttgtaaactt ccttttgcat cttataactg accagcgccc atgaataatc atgttgtttt
    18061 aaaaccagta tagctcttaa cagacaatat ccatcgccat ctacaggagc atattttaat
    18121 cctaatgcct ggttagtttc acgctgaata aaagatatac cagacaaatc aatgtcagaa
    18181 tccaaatcag atattcccgg cgtatttatc cgctctggct ggacacttat attttcatca
    18241 tcaaaacaat actcagaaaa aaacatcatc acacgcatta atgttttcaa tatattccat
    18301 aaagccatac caataataac ctttaaaatc tgtgatagac tctgaagctc tgcccggcaa
    18361 ggacactcta acaatatcaa tatctcgaac atattgtata gtatatgtaa tacctcctga
    18421 aacccctgaa taaatttgtg tatcattagt atgcaatgtt ctcataaata atgatagatc
    18481 attatcattc tgcatttcct caaattcttt ttctagtgat tcacattcca gacatggagt
    18541 atcctcttca tttactttac gtctgagttc attgtataac cgcataaaac acaccagtgt
    18601 gttttcaaaa agcgtgattg aatatgtttg atttttttcg tactcatttt ttatatcgtt
    18661 ccaggcctgt ttcaaataat tatttgcagt gcaactaatt ccatgtatat tactacccat
    18721 atatagcgtc tataaaattt aataaataat gacgccctag ttaaacttaa agtgcctggt
    18781 tcaactatca aaaatcgctc accctttttc acctgtttaa aatatgttca gcaacccatc
    18841 ttgatggcga cctcctctcc gcgatgattt caataacata ttctgtgttg gcatatgaaa
    18901 ttttgaggat taccctacac ttataggagt taccttacag gggttccttc aatttgtgtt
    18961 gatttacgcg agataacgct atggaattaa aggattatta cgccatcatg ggcgtgaaac
    19021 cgacggacga tctcaagaca atcaagaccg cctatcgtcg acttgcccgc aaataccatc
    19081 ctgatgtcag caaagaaccg gatgccgaag cccgcttcaa agaggtcgct gaagcctggg
    19141 aagtgttaag tgatgaacaa cgtcgcgctg agtatgatca gatgtggcaa catcgcaacg
    19201 atccgcaatt taaccgtcag ttccaccatg gcgacggtca gagttttaac gccgaagatt
    19261 ttgacgatat cttctcgtca attttcggtc agcatgcccg ccagagccgt caacgccccg
    19321 ccacacgcgg ccacgatatt gaaatcgaag tggcggtatt cctcgaagaa acgcttactg
    19381 agcataagcg taccatcagc tataacctgc cggtttataa cgcctttggc atgatcgaac
    19441 aggaaattcc gaaaacgctg aatgtgaaga tcccggcggg cgtcggtaat ggtcaacgca
    19501 tccgtctgaa aggccagggg acgccgggcg aaaacggcgg tccaaatggc gatttgtggc
    19561 tggtgattca tattgcgcca catccgctgt ttgatattgt cggccaggat ctggaaattg
    19621 tggtgccggt tagcccgtgg gaagcggcgc tgggtgctaa agtcaccgtt ccaacactga
    19681 aagaaagcat tttgctgact atcccgccag gcagccaggc cgggcaacga ttgcgcgtta
    19741 aaggcaaagg tctggtgagc aaaaaacaga ccggcgatct gtatgcggta ctgaaaatcg
    19801 tgatgccgcc gaaaccggat gaaaacactg ccgcgctgtg gcagcaactg gcagacgccc
    19861 agtcgtcttt tgatccacgt aaagattggg ggaaagcata atggctaatg ttacggtgac
    19921 ttttactatt accgaatttt gcctgcatac cggcatctct gaagaggagt tgaatgaaat
    19981 tgtcggtttg ggggtggttg aaccgcgtga gatccaggaa acaacctggg tatttgacga
    20041 ccatgccgcc attgtggtgc aacgcgcggt acgcctgcgt catgaactgg ctctggactg
    20101 gccggggatc gcggtggcgc tgacgttaat ggatgatatt gcgcacctga agcaggaaaa
    20161 ccgcctgctg cgccagcggc tttcccggtt tgtagctcat ccgtgatgtg tgtttgccgg
    20221 atgcgatgac gcatcttaac cggtctacaa agattacagt tcctgtgggc cggataagac
    20281 gttcacgccg catccggcaa ctgcgccata atgcctgatg cgacgctaaa cgcgtctttt
    20341 caggcgcaca aacgacgtta tctgttttgg tggtcgcact ccactaacac cagcaataac
    20401 tggcttagtg ccgcgtaaaa accaaagctg tcatactgac gacaacgcgc aacaaactct
    20461 ggtaaccatt gccacagcgc cgtcagtgtt ttttgccgca aactgtcgat tcttcgcgca
    20521 ggaacggtcc cctctcccag cgaaaaatgc agatggctga gcaattcgag atagatcgcc
    20581 agatgatctg ccggttcgtt gaaattgccg ctggtttcca tccctgcctc aactaacaag
    20641 cgtttaatct cttgctcgtc ctgtttgtag gccgatgcat acggcagcgc cgcttgtttg
    20701 tcggtcatca gaaacaggcc gcaaaagtca gcggccagtt ccagacgggc atcgtcacgt
    20761 actgtcagcg tggcaataca gttttccagc tcattcaccg ccgcagccag cggcggttcg
    20821 cttttcagca acgaaaacca ttcagccatc tgcgcactgg cgatttgcgt cagttgttca
    20881 tcgtccagct cacgggagaa caactgcgct agccaggcgt aaacacaggc aatctgttgt
    20941 gctgtcagcg tggtcatgat ttcacctgcg acgcgggaac atattcgcac tgcgccacca
    21001 tctccacggg gccgttaaac gccgtcacct gctccactgt tccgttgtac ttctcaattt
    21061 ccaccagcgt agtgtgcgca ctggtcgcct gcgccagctg cgatgtaccg atgtcgatgg
    21121 tcaacacgtt ggggttaccg tatttgcaca gcgcaccagg ctcgccgcct ttatctggat
    21181 cgtaccatgc cccttcgtga attcgtgcca cgccgggtgc atagcggtca gaaaccactg
    21241 cccctgccaa cacctgaccg cgagcgttaa agacgcgtac cacatcaccg ttacgaatac
    21301 cgcgcgcgct ggcatcctgc gggttaatga atactggctc tttacccgct accgtatatt
    21361 gctgacgcag cgtttccgac tcacataact gcgagtgaag tcggaaatcc ggatgcacag
    21421 attgcagatg caacggatac ttttgcgagc caggcccacc gtgggagcgt tcgattttct
    21481 caaaccacat cggatgcccc tgacaatcgt cgtagttcat atcggcgata gttttcgagt
    21541 agatctcaat caggccactc ggcgtgccca gcggttcgag atccggatct tcgcggaatg
    21601 cctggtggcg aacaaacatc tgcggatggt caaactcgac gtactctttg ttattccaga
    21661 agtcatcaaa cgctggcaga tgaacgccgc gtcctttgcc ttgctgtaca ccttcctgcc
    21721 agatgcgttt cagccagccc atttcgtcca gcccttcggt aaaggcttct tcgcgattaa
    21781 agcgacggca cagctcgcgg aaaatatcga agtcgttgcg cgcctcgaac tgcggcggca
    21841 ccacctgttt catggcgata atgccacggt tggagtgatt gccgtactgg tcgagatcgt
    21901 tacgctcaaa ctgcgtggtc gcaggcagta cgatatcggc aaagcggcag gttgaggtcc
    21961 actggttatc tatggcgata accgtttcca gcttgcgcaa gccttcaata atgcggttga
    22021 tctgctgatg gcgatggaat gggttagttc cggcaaaaat acacattttc agcggcggca
    22081 gttttaccga tttaccgttc cagttgatca ctttccccgg ttcgaggatc gcatcgataa
    22141 aacgggcaat cggaatagtg ctgctgtagc ctttgtagtc actgttgtcg tgaacaggcg
    22201 gaatcgacgt agagccggag aaaccactca gaataacgcc tttacgcccc ggcgtgcctg
    22261 cgccgttgta gtgccagcca aaaccaaaac caccacctgg caggccaatt tgccccagca
    22321 tcgccgccag aaccacaatc atccacgccc actgttcacc gtgctgcata cgctgcacgc
    22381 accagccagc aataatttgc gttctgttcg ccgccatctg ccgcgccagc ccacgaatgg
    22441 tttcggcatc aatgccggtc agtttttcag cccatgcggc atctttcggc tgaccgtctt
    22501 tctcacccag cagatacggc aggaactgct caaaacccac acagtagtta gcaaggaagt
    22561 ttttgtcgta caggttttca ctgtacagcg tatgtgccag cgccagttgc agcggcacgt
    22621 cagtttgcgg gttaaccgca atgtgcttca catgctcgcg ccccagatac tcatgggtgg
    22681 atgtgacaac cggatcgatg ctgatgacct caatttcacc ggcggcgact ttcgctttta
    22741 gctgcgcgta atattcataa acatcgtgat ccgggcacca ccagttcgct tgctggtttt
    22801 tcagcaaatc ggagccccac agcacaatgg ttttgctgtt ctgcaatacc agcggccagg
    22861 aggtttgctg ttcatacact tccatcgaac cgactacgcg cggcaggatc acctgcgcag
    22921 caccggtaga gtaatctccg cccgtaccaa cgctattacc atgcaaggca atagctttcg
    22981 ccagcatccc cgaagcgtta tggaacatcc ccgtcgattg ccaaccactg gcggtcagca
    23041 aggcactcgg cccgtgagtt ttctgcacgc gttccagttc ttcatagaac atgtcgaggg
    23101 cttcatccca gctcacgcgc acaaaacggt tatcaccgcg ctgggaggta tcgctgagat
    23161 ggcgcttacg cagccagtcc acgcgtacca tcggataacg aatacgcgcc gcgttgtgta
    23221 cgtgatccgg caatccggca atcattttcg acggatattt atccagttcg aacggttttg
    23281 ccgccacaaa gcgaccatcc ttcaccgtcg cgcggatagc cccccagtgc gacccggtaa
    23341 gaatgccctc tttcgagatg acagcgtcag tcgccgcttg cgccgcagtc gcacgtcgcg
    23401 gcgttaacaa tgacggcccc agcatcccgg cgacggttaa gccgccgagt tgtgccagaa
    23461 aacgccgacg tgatgcctga aagagatcgt tattgttcat tatttttctt ccttcttatc
    23521 gccgtgagcc ttacctgcgg tgtcagacgc attcatttgc agatatttca acaaggtgcg
    23581 ttcttcacgt ttatcgagac tggtaaagcc aatcatgccg ttgagcgtgc cgatccaacc
    23641 gttagcgtca aagtgggcga tttccggtgc gccgtggcac tggttacagg tgccgttgta
    23701 caacgaatcc gcataagccc agatcggttt gatatcgttc accatgtcgc ctttcttcat
    23761 ccacgcagtg gcctgcaact tgctccactc ggtattggtg tccgcaacgg tggttttctc
    23821 cagcgttttt acctgctgct gcacatcacc acgaatcgag gcaacaaaga tgcgtttacc
    23881 tgggaattgg gtgagtacac gctgacgtcc ggcgctttcc gtccagccgg taatttcaat
    23941 ttgcagccag tcgccgtcac gtttaaggac tttcacttcc gaagcaggca gcagagaacc
    24001 agaggcttct ttatcgcctt tcgccgcata aattggctta atatcaatag agtacagcgt
    24061 gtcaccactg tcattagcac tggcgcgcag ctcatcgaac tgcttacgga agccgctact
    24121 catatccggt aactggtggg caataccttt atgacagtcg atgcaggatt gattatcttt
    24181 cgctgccacc ttcatctgac gtgctgcttc aggatgctgc ttcgcatgat ccatcgcatc
    24241 gtagttatgg caggagcggc aggttgccga gttgttttct ttcattcgcg cccattcacg
    24301 ctcggcaagt
//
LOCUS       DACAPB010000054        23497 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.054, whole genome shotgun sequence.
ACCESSION   DACAPB010000054 DACAPB010000000
VERSION     DACAPB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.054"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1394)
                     /gene="prfC"
                     /locus_tag="IT904_001950"
     CDS             complement(<1..1394)
                     /gene="prfC"
                     /locus_tag="IT904_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP3191735.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYES"
     gene            complement(1485..2162)
                     /gene="yjjG"
                     /locus_tag="IT904_001951"
     CDS             complement(1485..2162)
                     /gene="yjjG"
                     /locus_tag="IT904_001951"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAP3191736.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(2177..2623)
                     /gene="rimI"
                     /locus_tag="IT904_001952"
     CDS             complement(2177..2623)
                     /gene="rimI"
                     /locus_tag="IT904_001952"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP3191737.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(2592..3005)
                     /gene="holD"
                     /locus_tag="IT904_001953"
     CDS             complement(2592..3005)
                     /gene="holD"
                     /locus_tag="IT904_001953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAP3191738.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            3108..4139
                     /gene="rsmC"
                     /locus_tag="IT904_001954"
     CDS             3108..4139
                     /gene="rsmC"
                     /locus_tag="IT904_001954"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAP3191739.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4440..4526
                     /locus_tag="IT904_001955"
     tRNA            4440..4526
                     /locus_tag="IT904_001955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4474..4476,aa:Leu,seq:cag)
     gene            complement(4565..4801)
                     /locus_tag="IT904_001956"
     CDS             complement(4565..4801)
                     /locus_tag="IT904_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAP3191740.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYILLPSCLALSAGIMLMLMNLNQG"
     gene            4942..5730
                     /gene="fhuF"
                     /locus_tag="IT904_001957"
     CDS             4942..5730
                     /gene="fhuF"
                     /locus_tag="IT904_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAP3191741.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHAIFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(5768..6445)
                     /gene="bglJ"
                     /locus_tag="IT904_001958"
     CDS             complement(5768..6445)
                     /gene="bglJ"
                     /locus_tag="IT904_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAP3191742.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGTPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(6403..7128)
                     /locus_tag="IT904_001959"
     CDS             complement(6403..7128)
                     /locus_tag="IT904_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP3191743.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            7747..8517
                     /locus_tag="IT904_001960"
     CDS             7747..8517
                     /locus_tag="IT904_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAP3191744.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            8508..8981
                     /locus_tag="IT904_001961"
     CDS             8508..8981
                     /locus_tag="IT904_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP3191745.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            9088..9627
                     /gene="dnaT"
                     /locus_tag="IT904_001962"
     CDS             9088..9627
                     /gene="dnaT"
                     /locus_tag="IT904_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAP3191746.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            9630..10367
                     /gene="dnaC"
                     /locus_tag="IT904_001963"
     CDS             9630..10367
                     /gene="dnaC"
                     /locus_tag="IT904_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAP3191747.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            10416..10910
                     /locus_tag="IT904_001964"
     CDS             10416..10910
                     /locus_tag="IT904_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAP3191748.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            11164..13455
                     /gene="opgB"
                     /locus_tag="IT904_001965"
     CDS             11164..13455
                     /gene="opgB"
                     /locus_tag="IT904_001965"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAP3191749.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(13595..14617)
                     /locus_tag="IT904_001966"
     CDS             complement(13595..14617)
                     /locus_tag="IT904_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate-5-dehydrogenase"
                     /protein_id="HAP3191750.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            14756..15670
                     /locus_tag="IT904_001967"
     CDS             14756..15670
                     /locus_tag="IT904_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3191751.1"
                     /translation="MSRSQNLRHNVINQVIDDMVRGHIPSLLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            15884..17245
                     /locus_tag="IT904_001968"
     CDS             15884..17245
                     /locus_tag="IT904_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191752.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(17294..18958)
                     /gene="tsr"
                     /locus_tag="IT904_001969"
     CDS             complement(17294..18958)
                     /gene="tsr"
                     /locus_tag="IT904_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP3191753.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMESASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            19335..21485
                     /gene="btsT"
                     /locus_tag="IT904_001970"
     CDS             19335..21485
                     /gene="btsT"
                     /locus_tag="IT904_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAP3191754.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            21535..21738
                     /locus_tag="IT904_001971"
     CDS             21535..21738
                     /locus_tag="IT904_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAP3191755.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            21749..22705
                     /gene="yjiA"
                     /locus_tag="IT904_001972"
     CDS             21749..22705
                     /gene="yjiA"
                     /locus_tag="IT904_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAP3191756.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(22739..23041)
                     /locus_tag="IT904_001973"
     CDS             complement(22739..23041)
                     /locus_tag="IT904_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HAP3191757.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(23028..23315)
                     /locus_tag="IT904_001974"
     CDS             complement(23028..23315)
                     /locus_tag="IT904_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HAP3191758.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
BASE COUNT         5623 a         6201 c         5792 g         5881 t
ORIGIN      
        1 gattcatata ctgcttcaac gttgtattcg ctcttcaggc gcgataccac cacatcaaac
       61 tgcagcacac caacagcacc aacgatcaaa tcgttgttgg agatcggacg gaacacctgc
      121 accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg cttcagcgga
      181 tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc ggtgaacttc
      241 atcatctcac cctgggtaaa ggtgtcgccg atctgaatag tgccgtggtt gtgcaggccg
      301 aggatatcgc ccggatacgc ttcttcaacg tgcgaacggt cgcccgccat aaaggtcagc
      361 gcgtcggaga taaccacatc tttcgcagta cgcacctggc gcagcttcat gcctttttca
      421 tatttaccgg atactacgcg cataaacgcc acgcggtcgc ggtgtttcgg gtccatgttg
      481 gcctgaattt taaatacgaa gccggtaaat ttgtcttcgc tcgcctctac ggtacgggta
      541 tcagtctgac gcggcatcgg cgcaggggcc cactccacca ggccatccag catatgatca
      601 acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc cgcaaggaac
      661 aattctttgt cgaactcgtt agacgcgcct ttcaccagtt ccagttcgtc acgtagctgc
      721 tgtgccagat cttcaccaac cgcagcgtcg agatccgggt tattcagccc tttaacaata
      781 cggacttcct gaatcgtgtg gcctttaccg ctctgataga gataggtttc gtctttataa
      841 aggtggtaaa cgcctttaaa cagcttgccg cagccaatcg gccaggtgat cggtgcgcag
      901 ccgattttca gctcgttctc aacttcatcg agcagttcca tcgggtcgcg gatatcacgg
      961 tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac ttccatcagc
     1021 ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca gcagtccacc
     1081 gccgtcaggg tacgataggt atcttccgag aagtcttcgt gccccggggt gtcgagcagg
     1141 ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga gatcccacgc
     1201 tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg gccttttact
     1261 gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat ggtagtctta
     1321 ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc ttgcaaataa
     1381 ggagacaacg tcataattct ttcttcttga gtaagcggca tcgcgccgcg cttgttgaaa
     1441 acgaaaaatt gcggctattt tacccatcaa cgggggggag gcaatcagtg tttacacagg
     1501 agctgctcca gttcgtgcaa cgatgaaacg gtccaggtgg gcgcgatgcc ttctggttgc
     1561 tcgcgatggt gcgcattcag ccagcaggtc gcaagcccgg cgttgatgcc accgagaata
     1621 tcggactcag cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt gcccgcctgt
     1681 tccagcgcat aatcgaaaat tttcttattc ggtttagcaa cgccaacttc ttcggaaatc
     1741 accagcaaat cgaagtaatc acgcaggccc gtacgttcca gacgcacttg ctgcaaggcg
     1801 ctaaagccgt tggtgatgat gccgattttg gcgttgccac gaatggcgtt aagcagagaa
     1861 accgcgcccg gcagcggcgt acagatttcc gccatcgcat taataaaggc ttcgttgagt
     1921 ttacccggct cgacgttcag ccgttcggcc cagctctcaa accgcccgtg ctgaagctgt
     1981 aatgaagtga tcgcgccgtt ttgataatcc acccacagtg gcttgttaac ggcctgatag
     2041 tcctgaaaat cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg ctgcaggccg
     2101 gtgaatgagt caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca gtcccacttc
     2161 attattccac cttgtattac atactgattg gcaacgccat gatgatggcg tcttcgcgac
     2221 cgtccgtggt ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct aaactttcgt
     2281 acagggcaat ggcggcagcg tttgaggcac ggacttccag ccatagcgtc gccacaccgc
     2341 gtttttccag ttcgtcgatc agatgttcca gcagcgccct tcccaatccc tgacgctgat
     2401 agtcgggatc gaccgcaata ttgaacaatg tagcttcatc cagcaccact tgcgtaatcg
     2461 caaacgccgc cattttgccg ttttgcgtta actgaaagtt gagataacgc tcgccctggt
     2521 tgctggcaaa ggttttttca ctccacggaa aggcgtgggc gcgttgttca atgtggtaag
     2581 ccgccggtaa atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca aatctgttgc
     2641 cataacgcgg cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg tgatgccacc
     2701 tgagcgcctt ccagtgatag cggttcgtca gtacccaacc gccagctgtt gcagcgactg
     2761 ccttgcggca gcatcgcgat tttttctggc gtcagttgca gcacctggtc ggggctgacg
     2821 gttaatgcgc gcagaacatc gctcactaaa ggatcagtca gggcgggaag atcgtttgcc
     2881 accatcacca gacggacgtg tgccgggatc gcaatggcaa tctccccctg caacgcgcca
     2941 gggcgacgca gcgaccactg ggtaatgccc agttgctgta actgccagtc tcgtcgggat
     3001 gtcatagcga atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt cgataccgcg
     3061 ccaacatatg gctataatcg ccgccagtat caattgagga gcattccatg tctgcattta
     3121 ccccggcaag tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc cgtattctgt
     3181 ttgccggaga cttacaggat gacctgcccg cgcgtttaga taccgcggcc agccgtgctc
     3241 atacccagca attccaccac tggcaggtgt taagccgcca gatgggggat aacgcccgtt
     3301 ttagtctggt cgccacggcg gatgacgtcg cagattgcga tacgctgatt tactactggc
     3361 cgaagaacaa accggaagcc cagttccagt tgatgaattt actttctctg ctgccagtgg
     3421 ggacagatat ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgcc gagcagatgc
     3481 tggcagatta tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc ctctattttg
     3541 gtcgtctgga aaaacagccg gtatttgatg ccgataaatt ctggggcgaa tacagcgtcg
     3601 atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg gatgtcggta
     3661 gccagttgct gctctcgacg ttaactccgc acacgaaagg taaagtgctg gatgtcggct
     3721 gtggcgcggg tgtgctttca gttgcctttg cgcgccattc gccgaaaatc cgtctcacct
     3781 tgtgcgatgt ctctgccccg gcggtagaag ccagccgcgc gacgcttgca gccaacggtg
     3841 ttgaaggtga agtctttgcc agcaacgtct tttccgaggt gaaaggtcgt tttgatatga
     3901 tcatctccaa cccgccgttc cacgatggga tgcaaaccag cctggatgcg gcgcaaacgc
     3961 tgattcgcgg tgcggtgcgt catcttaata gcggcggcga gctgcgaatt gtagcgaacg
     4021 ccttcctgcc ttatccggac gtgctggatg agacatttgg cttccatgaa gttatcgcgc
     4081 aaaccgggcg cttcaaggtg tatcgcgcca ttatgacccg ccaggcgaag aaaggttaag
     4141 tatcccgccg caagccttgc cggatgcggc gtaaacgcct tatccgacat accaaacctg
     4201 cacgttagcc cttgtaggcc agataagacg cgccagcgtc gcatctggca tctgcccccg
     4261 aatgccgtgt cggatgcggc gtgaacgcct tatccgacct acaaacgaat tatccacaat
     4321 ccgctgatgg ccatttttcc agcaattaca cctccggcga taattaacta ttgacgaaaa
     4381 gctgaaaacc actagaatgc gcctccgtgg tagcaattct ttttaagaat tgatggtatg
     4441 cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgtcctt acggacgtgg
     4501 gggttcaagt cccccccctc gcaccaatta tctttacttc ctttcttgtt tcttccttga
     4561 tttttcatcc ctgattcaaa ttcattagca tcagcatgat gccagcacta agcgccaggc
     4621 acgatggtag caaaatgtaa tgccgtaact gtttgtgata cagcatcacc ggcgtcaata
     4681 tcaatcccat cagaatgaca atccgccact gatccggcga taaatccgca tacatcagcc
     4741 ctgcaatcag caatcccggc agcaacactc cccagccact gcccagcgta cgttgcaaca
     4801 tggtttcatc tctttcattg ataatgataa ccgatatcat atgataattt ttatcatttg
     4861 caagccagag aaatcccttg ctatcgggta aacctatcgc taggattagc aatcattatc
     4921 atttagatta ctatcacgat tatggcctat cgttccgcac cgctctatga agatatcatc
     4981 tggcgaacgc atctccagcc acaggatgct gggcttgcac aagccgtacg tgcgacgatc
     5041 gccgaacatc gtgaacattt gctggagttt atccgcctgg atgaacccgc tccgcttaac
     5101 gccatgacgc tggcgcaatg gtcatcaccg aatgcgctaa gttctctact cgcggtctat
     5161 tccgatcata tctatcgcaa ccaaccaacg atgatccgcg agaacaaacc gctgatctca
     5221 ctatgggcac aatggtatat cggcctgatg gtgccaccat taatgctggc gctactgacg
     5281 caggaaaaag cattagatgt gtcgccggaa catttccatg ctgagtttca cgaaaccggc
     5341 cgtgccgcct gtttctgggt cgatgtgtgt gaagataaaa acgcaacacc acattcgccg
     5401 cagcagcgaa tggaaacgtt aatcagccag gcgctggttc cggttgtgca ggcactagaa
     5461 gcgaccggag agatcaacgg caaacttatc tggagtaata ccggttattt gatcaactgg
     5521 tatctcactg agatgaagca actgctcggc gaggcgacgg ttgaatcgct gcgccatgcc
     5581 atcttttttg agaaaacgct cacgaacggt gaagataatc cactctggcg taccgtggta
     5641 ctgcgcgacg gcctgctggt gcgccgcact tgctgccagc gttatcgctt accggatgtg
     5701 cagcaatgtg gcgattgtac gctgaaatga aagcactgcc ggggaagtaa acccggcatc
     5761 atgcggatta atagggatgc aacacattac ttgcttcgca atgccgcata cataacagaa
     5821 tatctgcggc ctccaacaat cctgcatcag aactgacgcc cagtttcgac atcacattaa
     5881 atttatgcgc gcggatcgtt ttgatattgc gtttaagctg ctcggcaatt tgtgtcattg
     5941 agtagccacg cgacataaag cgcaatattt ctctctccgt cgggcttagc gtccggcttt
     6001 ggttaatgta ccactgattg ttcagtcggt cggtcgcctg acgtacacca tttaatgaca
     6061 aaaagagttc ctgatgaaaa atctccagcg ttgacgcttt acttaataca ccgtccagcg
     6121 gtgatggcga caatgaacca atcagccgag cttcaatatc atcatccgca ataactaaac
     6181 gccgggtacg cggaaactta atcgccagtt cagtcaggca agataatccc tctctgcgct
     6241 cacttctcat ggcagaaaaa gaaaaaatga ccgccgcaaa cgagatccgc gacatggcat
     6301 cctgaaatga ctcctgactg gtataggtat ggagcttata aggggtgccc gcaaactttc
     6361 tgaataaact ctcaataccg atactactca tgacgcattt ttctatgagt gcgacatttc
     6421 tcttcttaat tcggctgtgt tccattctgc ggcatctcca taaacagaag gcaagttaag
     6481 ttctcttgca ccctgcgcgc tatttatcca tgcatacatt tcagcgttgc tgtgaattgc
     6541 cagtcgacgc atcgcactgt ttttttgggc gctgatagtt ttattacttt tcttaagcaa
     6601 tgaagctatc tggttgatgc cccatccttt acctaatagc cgcagtacct tgcgctctga
     6661 aagagtgaga atgacggagc gatagctaaa atcatgagtt tcagtcattg ccggggaggt
     6721 cagcatggcg ctgatacgct ctgcgtgcgt attgccggaa cgtacggtct tgaccagatt
     6781 ctcaatgggt tcaacatcag acaataacgt cgccgtaggg cacatgagca gttctactgc
     6841 ctgggaatac caggagcgtg acaccatgaa aacccagtga atttcccgat attgtgagat
     6901 taaagaataa taatgttcac aaacagaacg tggatcttca ctttcaccgg ctaaatcagc
     6961 aatgactaat ccggaacgac gtaattgtaa taaggtaagg tcctccgcag aggcgctgga
     7021 tattatttca tattcaggga agtgagtcct catgacctct tttaaccctg cttgcataac
     7081 aggtatttta ctgataacaa ttccattttt gcagcatcct ggcaacatat ttcctccaca
     7141 tccactgtct aaaaattatg atggtttatt caggatattc attccttaaa tgtttccagc
     7201 aaattaatag attaagcgaa taaattatcc ttagcattca ctttcatatc agcatactcc
     7261 tgctgcacaa caactccact gcatgtgtca gaaggttagc atcaaatgat aacctggtga
     7321 aatagcctgg agacatttaa aatatctaca ttaagttgtt aggaatactt aatgaaaaac
     7381 agaaatcaca ttcatttaat ttataattaa atgatatccc ttcccgcaga atgaaaaatc
     7441 acacaaccaa tatttattca atgaaatctg atggttttaa cttgttaaca ttaaaaaacg
     7501 tgcccggtta tctattattc tggttcgtaa aggacaaaca ataaattcat tatggtgata
     7561 atattcatgt gatagtttgt ttgcgacgag agataattcg ctttttatca ccgagcttgt
     7621 cccgataacc aggattaatc agcgcaattt tgatcttaac ctaccgcaaa acaaccgtgc
     7681 ttcatgatac aaatggattc tcattatctg aacaatacgc agcacgtcta tgacaaaggg
     7741 cgagttatgc aaactgagca acagcgagcc gtaacacggc tttgtatcca gtgtggatta
     7801 tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg agctttcctc acgactgggt
     7861 cgggcgttgg ggatggacag cgtcgaaagt tctatctctt cgaacgccat agtgctgaca
     7921 accattaaag atgggcaatg cctgacatcg acacgtaaaa atcacgatcg cggcattaat
     7981 atgcatgtgg tgactgaagt ccagcacatt gtgattctgg cggagcacca tctgctggat
     8041 tacaaaggcg tagagaaacg atttagccaa attcagccat tacgttaccc aagatggctg
     8101 gtagccttaa tggttggcct ttcttgcgcc tgtttctgca aactcaataa aggtggctgg
     8161 gatggtgccg tcatcacctt ctttgccagt acggccgcga tgtatatccg ccagctgctg
     8221 gcacaacgtc atcttcatcc gcagatcaac ttttgcctca ccgctttcgc cgccaccacc
     8281 atttccggat tgcttttgca acttcccact ttcagcaata cacccactat cgcgatggcc
     8341 gccagcgttc tgctgctggt gccgggattt ccgttgatta atgccgtcgc cgatatgttt
     8401 aaaggccaca tcaataccgg actggcacgc tgggcgatcg ccagtctgct gacactggct
     8461 acctgcgtcg gcgtagtgat ggcactgacg atttgggggc tacgcggatg ggtgtgatcg
     8521 aatttctgtt agcgttggcg caggatatga tcctcgccgc cattcctgcg gtcggctttg
     8581 cgatggtgtt caacgttccc gttcgggcgt tacgctggtg tgcgctgctt ggcgcgattg
     8641 gtcatggttc acgaatgatc ttaatgacca gcgggctgaa tattgagtgg tcaactttta
     8701 tggcttctat gctggtcggc accattggta ttcaatggtc gcgctggtat ctggcgcatc
     8761 cgaaagtgtt taccgtggcg gccgttatcc ctatgttccc gggcatatcg gcttataccg
     8821 caatgatttc ggcggtaaaa atcagccagt taggttacag cgaaccgttg atgattaccc
     8881 tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc gttatccatc ggtctttcca
     8941 ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata aaattgcctc gcttaagcac
     9001 acggatgaga gacagcctcc tctcctccgt gtgttactat aaaagttatc tcccttctcg
     9061 ttcatcgttc catatttgag aaacagtatg tcttccagag ttttgacccc ggacgtcgtt
     9121 ggtattgacg ccctggtaca cgatcaccaa accgttctgg caaaagctga aggcggtgtg
     9181 gttgccgtat ttgctaacaa tgccccggcg ttttatgccg tcacgcctgc acgcctggct
     9241 gaactgctgg cgctggaaga aaagctggcg cgtccgggaa gcgatgtcgc tctggacgat
     9301 caactctatc aggaaccgca agccgctccc gttgctgtcc ccatggggaa attcgccatg
     9361 tatccggact ggcaacccga tgccgacttt atccgcctgg cggcgctatg gggcgtggcg
     9421 ctaagagagc cggtgaccac cgaagaactg gcctcattca ttgcctactg gcaggcggaa
     9481 ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt
     9541 cgtgccagca acggcggact gccgaaacga gatgtgaata cggtcagcga acctgacagc
     9601 caaattccac caggattcag agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc
     9661 tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa aacgggtgaa gagctactgg
     9721 cctggcagaa agaacaaggg gcgatccgct ccgccgctct cgaacgtgaa aatcgggcga
     9781 tgaaaatgca gcgcaccttt aaccgctccg gtattcgtcc actgcatcag aactgctcct
     9841 ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt
     9901 atgtcgaaga gtttgacggc aacatcgcca gcttcatctt ttctggtaag ccaggaaccg
     9961 gcaaaaacca tctggcggcg gcaatctgca acgagctgct gctacgcggt aaatccgtat
    10021 tgatcatcac cgtggccgat attatgtcgg cgatgaaaga taccttcagg aatagcggta
    10081 ccagtgaaga acaactgctt aacgatctga gcaacgttga tctgctggtg atcgatgaga
    10141 tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc
    10201 gctcttcttc caaacgccca accgggatgc tgaccaacag taatatggaa gagatgacca
    10261 agttgctggg cgaacgcgtg atggaccgta tgcgcctggg taacagtttg tgggtgatct
    10321 tcaactggga tagctaccgt agccgggtaa caggtaaaga gtattaatcc ccgtttctgg
    10381 cggtatactg cgacacaatt ccaccttgag taatgatgat gaaaactgtt aaacatcttc
    10441 tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga
    10501 aagatgcgct ctccagcgcc gccagcgaac ttggcaacca aaacagcacg acacaggaag
    10561 gcggttggtc gctcgcgtca ttaactaact tgcttagcag cggaaaccag gccttaagcg
    10621 cagataacat gaacaatgcc gcaggcattc tgcaatactg cgcgaagcaa aagctggcgt
    10681 cggtaaccga tgccgaaaac atcaagaatc aggtactgga aaaactgggc ctgaacagtg
    10741 aagagcaaaa agaagacacc aactatctgg atggcattca gggtttgctg aaaactaaag
    10801 atggtcagca actcaatctg gataatatcg gaacgactcc gctggcagaa aaggtgaaaa
    10861 ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt catttcctga accgcgaaac
    10921 gccgcgtttt agctgttacc cggctgacgc ggctctctcc tgcccttttc cctgctcctg
    10981 gacggttttc cccctgttac acgggaagcg accgtaatca aaaaagtctg tttataaact
    11041 aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg cgacgccatt
    11101 acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc
    11161 ccgttgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg
    11221 aaagcgggac gtaacacctg gtggtttgca gccacgttaa cggtgctggg gctatttgtc
    11281 gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat taacgacgcg
    11341 gttctctata ccttaaccaa cagcctgacc ggtgctggtg tcagcaaata cattcttccg
    11401 ggtatcggca ttgtgctggg gctgacggcg gtgttcggtg cgctgggctg gatcctgcgc
    11461 cgtcgtcgcc atcatccgca ccattttggt tacagcctgc tggcactctt actggcgctg
    11521 ggttcagtgg atgccagccc ggcatttcgt cagataacgg aactggtgaa atcccagtca
    11581 cgcgatggcg acccggactt tgcggcttat tataaagagc cgtcgaaaac tatccctgac
    11641 ccgaaactca acctggttta tatctacggc gaaagtctcg agcggaccta ttttgataac
    11701 gaagctttcc cggatctcac gcctgaactg ggcgcgttga aaaatgaagg cctggatttc
    11761 agccacacgc agcagctgcc aggaacggat tacacgattg cgggcatggt ggcttctcag
    11821 tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct ccgcctctgt ctccagcttc
    11881 ttcccgcaga acatctgtct gggcgatatc ctgaaaaact cgggttatca gaactatttc
    11941 gtgcagggcg cgaatctgcg ttttgccggt aaagatgtgt tcctgaagtc gcatggcttc
    12001 gaccacttat acggctcaga agagctgaaa agcgtggtgg ccgacccgca ctatcgcaac
    12061 gactggggat tctacgacga taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt
    12121 tcccgctcag gtcagcgatt ctcactgttt accctgacag tcgataccca tcacccggat
    12181 ggttttatct cgcgtacctg taaccgcaaa aaatatgatt ttgacggtaa gccgaaccag
    12241 tcattcagcg cggtaagttg cagccaggag aatatcgcgg cgtttatcaa caaaatcaaa
    12301 gcgtcaccgt ggtttaaaga taccgtcatc gtcgtctctt ctgaccattt agcgatgaac
    12361 aacacggcgt ggaaatacct taataagcag gatcgcaata acctgttttt tgtcattcgt
    12421 ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta acacgatgga taacggcgcg
    12481 acggtgctgg acattctcgg tggcgataac tatctcggac ttggtcgtag cagtttatcc
    12541 gggcagtcaa tgtcggaaat cttcctcaat atcaaagaga aaacattggc gtggaagccg
    12601 gatatcatcc gcctgtggaa attccctaaa gagatgaaag agttcaccat cgaccagcag
    12661 aaaaacatga ttgccttctc gggtagccat ttccgtttgc cactgctgtt gcgggtttca
    12721 gacaaacgcg tggaaccgct gccggaaagc gaatactcag caccgctgcg tttccagctg
    12781 gccgatttcg ctccacgcga caatttcgtc tgggttgacc gttgctataa gatggcacaa
    12841 ctctgggctc cggaactggc actctccacc gactggtgtg tctcgcaagg gcagcttggc
    12901 ggtcagcaaa ttgttcagca tgttgacaaa acaacatggc agggcaaaac ggcatttaaa
    12961 gatacggtca tcgacatggc gcgttataaa ggcaatgtcg atacgctgaa aattgttgat
    13021 aacgatattc gctacaaagc cgacagtttc atcttcaacg tcgccggtgc gccagaagag
    13081 gtgaaacagt ttagtgggat ttcccgtcca gagtcgtggg gccgctggtc caacgcgcag
    13141 ctgggcgatg aagtaaaaat cgagtacaag catccgctgc cgaagaaatt tgacctggtg
    13201 attaccgcca aagcatacgg caataacgcc agccgtccta ttccggtacg cgtaggcaat
    13261 gaagaacaaa ctcttgtgct gggcaatgaa gtgaccacca ccacactgca tttcgataac
    13321 ccaaccgatg ccgacacact ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg
    13381 aatatcctcg gacactcgcc gcgtaagctc gggatcggca tggtggaaat taaagttgtg
    13441 gaacgtgaag ggtaatttct gactgatgtg taggtcgcat aaggtgcgac ctacacatct
    13501 tctccggcct acaaaacatc attgagaaac agaaaaaaaa cagcgcccaa cacttccccg
    13561 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
    13621 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
    13681 gatgggttaa catcatgtcg gcagtgattt ttccttccgc catcagtcga ccgactttag
    13741 caaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
    13801 ccggatcgga gaactgcaac tcacctttaa acaggccgac aaataccacc gtgccgccgt
    13861 gacgaatcaa attcacggtg ttattcatcg catgttgatt acctgtcgcg tcgatcactt
    13921 tctgcgccag cgaaccacca aactgcgccc gtagctgcgc gtcaaaatct tcggctgacg
    13981 ggtccagtaa aggtaattcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
    14041 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
    14101 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
    14161 gagcgctaat agcgaaaggt tcaatcaatg ctgccgcctg cgggtcaata ccgtctgcgg
    14221 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
    14281 caatgactga aattttttca cagcaattgg tacgcccgct tttacacgcc gggcattgct
    14341 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
    14401 tacccagccc aacaatctcc ccacatattt catggcctaa aacacgtgga taactaaaaa
    14461 atggttgatt tccaccccag gcatgaatat cggtaccaca aatcccgaca gacttaattt
    14521 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
    14581 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctctcc attttttgtt
    14641 gccttagata tcaataaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
    14701 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
    14761 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggtgcgggg
    14821 tcatatccct tccctacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
    14881 cactacagtg cgtcatattc tcagccactt acgcgaatgc ggcgtcttaa cgcaggttgg
    14941 aaacgactat gtgattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
    15001 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
    15061 ccagttacgc cctggggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
    15121 cccggttgta gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
    15181 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
    15241 actacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
    15301 tccccgctgg ctgcaggcga aaaccatgct cgaacgtcat cggctactgc gtgacaacat
    15361 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
    15421 agctgccgac aatatctttt ttgatcaatc tcttgagatc atctcagtca tctttcattt
    15481 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgagca
    15541 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
    15601 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
    15661 tgctcattaa gtacggatta aaaacagcaa actattagcg gtgcacggtt ttatcaaaag
    15721 ataaataccg ggaaattccc gcttacctat gctcacaatc agtcgactaa aaatgttttt
    15781 gtgagcggta gtaaagtcct aaaactttaa cctgccgtta atgtcaacct tgcgtaatgc
    15841 acaattacgt aaacaattaa aattataaat acaggagtcc ggcgtggaaa aagaaaatat
    15901 caccctcgat ccgcgttctt catttactcc atcttcgtcg gcagatattc ccgtgccacc
    15961 agatggatta gttcaacgca gtacccgaat taaacgcatt caaaccaccg ccatgttgtt
    16021 attatttttt gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt
    16081 aacgattcgt gaagaattgg gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt
    16141 ttcactcgct tacgggattg cgcaacttcc ttgcggccca ctattggatc gtaaaggccc
    16201 acgcctgatg ctgggactgg ggatgttctt ctggtcactg ttccaggcca tgtctggcat
    16261 ggtgcacagc tttacgcagt tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc
    16321 gatgaaccca tgtggcgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg
    16381 cccgatgggc ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct
    16441 ggcggcgatg atgctggtga tgggctggcg tgggatgttt attaccattg gtgtgctggg
    16501 gatttttctc gccattggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac
    16561 tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag
    16621 ttttgccgaa tggcgcagcc tgttccgtaa ccgcacaatg tggggaatga tgctcggatt
    16681 cagtggcatc aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac
    16741 agcctataac ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg
    16801 ggctgccggg atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc
    16861 tccgattaaa agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac
    16921 gctggtcgta ccgcaagcga caacatccat gacggcggtt ctgctgattg gcatggcact
    16981 gttctgtatt cactttgccg gaacatcctg ctggggcttg atccacgtcg cagttgcttc
    17041 tcgcatgacg gcgtcggtgg gcagtatcca gaactttgcc agcttcatct gcgcctcttt
    17101 tgcgccgatc attactggtt ttattgttga taccacccac tcattccgtc tggcactaat
    17161 catctgcggt tgcgtcaccg cagcgggagc actggcgtac atcttcctgg ttcgtcagcc
    17221 gatcaacgac ccgcgtaaag attaaactcc ctatgcccga taacattttg cttatcgggc
    17281 atttttatgg cgattaaaac gtttcccagt tctccccgct atctgccact gccattttac
    17341 gcggcgtagc tggcgtcacg ttttttacca cagccgatgt ttcacgctgc tgttgctgct
    17401 gttgctgaat ccggaacact gccacagctt cggtcaggcg actggcctgc tcttccagcg
    17461 cggcggcggc agcggcagat tcttccacca gcgcagcgtt ctgttgagtt acccggtcca
    17521 tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca gaagcagaag
    17581 caatttcgcc cataatgtcc gtcacgcggg tcacggcact aacaatctcc gccattgttt
    17641 cccctgcgct ttcgaccagc gtagagccaa catctacttt ccccaccgag tcttcaatca
    17701 ggcttttaat ttcacgagcc gcctgagcgc tgcgctgggc cagattacgc acttcacccg
    17761 cgaccaccgc aaaaccgcgc ccttgctcac ccgcgcgcgc tgcttcaacc gccgcgttca
    17821 aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata tcggcgattt
    17881 tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatctacc actttgccgc
    17941 cgcgttgcgc cgtttcagaa gcacttaacg ccaggtggct ggcctggcgc gcattttcgg
    18001 cgttctgttt caccgttgcg gtcagttgct ccatgctggc tgccgtctct tccagcgaag
    18061 cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt tcgctggcac
    18121 cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc agctctccct
    18181 gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc acctcaatcg
    18241 gtttcaccag atcgccgcct gcaatatgac gaatgctgtc aatcaggcga ttcattggcg
    18301 ctaccaacga ggctttaata ccgaaccaga cggcgaagat gactgccagt acgacgatca
    18361 tcacgcccac cagaatccac atcgcctggc tgtaggaggc attattatcg ctgacggcga
    18421 tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg aaaccgtcct
    18481 gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct aacagttgga
    18541 tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct gccgctgcgg
    18601 cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc cgcccagttt ttttccgcct
    18661 gtttcagcga aatactggca ctctccatca gctcagcaac ggttgaaccg ctaccaatat
    18721 tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc gtctgcaaca
    18781 acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt aaaacagtga
    18841 aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc agttgtaaaa
    18901 ggccaaaaac ggccaaaacc agcagtaagc tggtcacaat tttgatacgt tttaacatgg
    18961 tttctctttc ctgtggaccg atgcaagatt ttcggccagg aaaggaaaaa ctttatgaat
    19021 atcccgcgga gattacgccg agtaatttta ttcacactct gaatttaaaa agccattatt
    19081 tacataaatt actcacaata taaatatgtg atatgaatca catatttatc gtcacttaaa
    19141 cgacgccttt gccgctcaac cgcaaaactg accgcttaca tccctaaaat aaccactcag
    19201 ttatttacct tactttacgc gcgcgtaact ctggcaacat cactacagga tagcggtcaa
    19261 tttacctcct caaacgcaac gcaaacctag aacggcttcg gccaactatt aatcaataca
    19321 tgccaggttt tactatggat actaaaaaga tattcaagca cataccctgg gtgattctcg
    19381 gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg
    19441 ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc
    19501 tgtacatcgc ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta
    19561 acaacgacgg tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg
    19621 ccgctatcgc cggtgcaggt ccgctggtgg gtccggttct cgccgcgcag atgggctacc
    19681 tgcctggcac gctgtggctg ctggcggggg tcgtgctggc cggtgcggtt caggacttta
    19741 tggtgctgtt tatctcctct cgccgtaatg gcgcatctct tggtgagatg atcaaagaag
    19801 agatgggacc agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca
    19861 tcctcgccgt cctggcgctg attgtggtta aagccctggc cgaaagtccg tggggtgtct
    19921 tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg tatctacatg cgctttatcc
    19981 gtccggggcg tgtgggtgaa gtctctgtca ttggtatcgt gctgctggtt gcctctatct
    20041 acttcggtgg cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca
    20101 ccaccattac cttcgcgctg attggctatg cgtttgtttc cgcactgctg ccggtgtggc
    20161 tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc
    20221 tggcgctggg tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca
    20281 ttgacggtac tggcccgctg tggaaaggcg ctctgttccc gttcctgttc atcaccatcg
    20341 cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc
    20401 tggctaacga aaccgacgcg cgtttcattg gctacggcgc aatgctgatg gagtccttcg
    20461 tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc gggtctttac ttcgcgatga
    20521 ataccccgcc tgcgggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga
    20581 acgcgccgat catcatggcg cagctgaaag acgttaccgc acacgcggcg gcgaccgtca
    20641 gctcctgggg cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg
    20701 agccttctgt cctgaaccgt gcaggtggtg cgccgacgct ggcggtaggt atcgctcatg
    20761 tgttccacaa agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt
    20821 tcgaagccct gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgctttatgc
    20881 tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg
    20941 gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg
    21001 tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc
    21061 tggcagccgt agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat
    21121 acatctgggt aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg
    21181 gtctgaaact gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt
    21241 acaaagagaa gattgctaac ggtactgacc tgacggcgca gcagattgcc aacatgaacc
    21301 acatcgttgt gaacaactac accaacgcgg gcctgagtat tctgttcctg attgtggtgt
    21361 acagcatcat cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta
    21421 ctgacaaaga aacaccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc
    21481 actaaccgtg tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt
    21541 ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg caaagatgtt gattggcatt
    21601 ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg
    21661 agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc
    21721 ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg
    21781 ttttttaggc gcgggaaaaa ccaccctgct gcgccatatt cttaacgaac aacacggcta
    21841 caagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc aactaattgg
    21901 cgaccgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa
    21961 cgagctggaa gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt
    22021 tgaccgtctg gtcattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt
    22081 tttctcccat gagattttat gccagcgtta cctgctggac ggtgtgattg cgctggtgga
    22141 tgcggtacat gccgacgagc agatgaacca gttcaccatc gcccagtccc aggttggcta
    22201 cgccgaccgt attctgctga ccaaaaccga cgtcgcaggc gaagcagaaa aactgcgtga
    22261 acgcctggcg cgcatcaacg cccgtgcacc ggtctacacc gtcacccacg gcgacatcga
    22321 cctgggcctg ctgttcaaca ccaacggttt tatgctggaa gagaacgtcg tcagcaccaa
    22381 accgcgtttc cactttatcg cggataaaca aaacgatatt tcgtcgattg tggtggaact
    22441 ggattacccg gtagatatca gcgaagtttc ccgggtgatg gaaaacctgc ttctggaatc
    22501 ggcagataaa ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct
    22561 gctgttccag ggcgtccagc gcctctacag cgccgactgg gaccgcccgt ggggcgatga
    22621 aaaaccgcat agcacaatgg tgtttatcgg gattcagttg ccggaggaag agattcgggc
    22681 agcgtttgcg gggttgagga agtaattttg gatgatggcc gccatgtggc ggcctgaatt
    22741 attttttgtt ctgatcgcgc agcatcgctt cacgtaaaat tgcgttcagg cgagtttgat
    22801 agcctttacc tggtcgcttc aaccattcca tcacatcagc atcaatacgc accgaagcct
    22861 gagttttcaa tggccgataa aacttaccgc gttcggcgtt agaccattgc tcatcgctgg
    22921 aaggtgggat atcactgtaa tcaatatctt cgtcggattt attcgtcagc gccttcagtt
    22981 cggcctcatg ctgcacatcg agtgtagagg aggtccctcg tttatgttta accatgctca
    23041 tagcgattcc tctctttacg atctgctttt cgtgcactga tgattcgaat aatttcattt
    23101 ccgctttcga aacggatggt atgcgcaacc agaataacca cgatgccgta aaccaggccg
    23161 attgtctgcc agcgatactc accgttttct atgcgttctt gctgtgacaa atgttgtgga
    23221 tcatcaaaca ataacaccgc atcttcaaag cggatgccat gtttcacccg attacttttg
    23281 gccttgtttt catcccattc aaactccatt ggcataattg ccatccctaa ttgtatatac
    23341 aaaaatgtat ctacaattat tctgcaatta tagcgttgat gttggaaata gcatcacgtt
    23401 tcgaataatc aaccatgaaa ttgacaatga gtaagttgga cttgattcat ccaatagaat
    23461 ttctcgcctg acatggcaca ctaaacacct cgctgtc
//
LOCUS       DACAPB010000055        23352 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.055, whole genome shotgun sequence.
ACCESSION   DACAPB010000055 DACAPB010000000
VERSION     DACAPB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.055"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            223..666
                     /gene="yjdN"
                     /locus_tag="IT904_001975"
     CDS             223..666
                     /gene="yjdN"
                     /locus_tag="IT904_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAP3191759.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            799..1587
                     /gene="phnC"
                     /locus_tag="IT904_001976"
     CDS             799..1587
                     /gene="phnC"
                     /locus_tag="IT904_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP3191760.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            1612..2628
                     /gene="phnD"
                     /locus_tag="IT904_001977"
     CDS             1612..2628
                     /gene="phnD"
                     /locus_tag="IT904_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3191761.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            2734..3513
                     /gene="phnE"
                     /locus_tag="IT904_001978"
     CDS             2734..3513
                     /gene="phnE"
                     /locus_tag="IT904_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP3191762.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYFTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3534..4259
                     /gene="phnF"
                     /locus_tag="IT904_001979"
     CDS             3534..4259
                     /gene="phnF"
                     /locus_tag="IT904_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAP3191763.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4260..4712
                     /gene="phnG"
                     /locus_tag="IT904_001980"
     CDS             4260..4712
                     /gene="phnG"
                     /locus_tag="IT904_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAP3191764.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVQGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            4709..5293
                     /gene="phnH"
                     /locus_tag="IT904_001981"
     CDS             4709..5293
                     /gene="phnH"
                     /locus_tag="IT904_001981"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAP3191765.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVSSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5293..6357
                     /gene="phnI"
                     /locus_tag="IT904_001982"
     CDS             5293..6357
                     /gene="phnI"
                     /locus_tag="IT904_001982"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAP3191766.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPGEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6350..7195
                     /gene="phnJ"
                     /locus_tag="IT904_001983"
     CDS             6350..7195
                     /gene="phnJ"
                     /locus_tag="IT904_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAP3191767.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGTDDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7192..7950
                     /gene="phnK"
                     /locus_tag="IT904_001984"
     CDS             7192..7950
                     /gene="phnK"
                     /locus_tag="IT904_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAP3191768.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8061..8741
                     /gene="phnL"
                     /locus_tag="IT904_001985"
     CDS             8061..8741
                     /gene="phnL"
                     /locus_tag="IT904_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAP3191769.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            8738..9874
                     /gene="phnM"
                     /locus_tag="IT904_001986"
     CDS             8738..9874
                     /gene="phnM"
                     /locus_tag="IT904_001986"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAP3191770.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQNGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            9874..10431
                     /gene="phnN"
                     /locus_tag="IT904_001987"
     CDS             9874..10431
                     /gene="phnN"
                     /locus_tag="IT904_001987"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAP3191771.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10418..10852
                     /gene="phnO"
                     /locus_tag="IT904_001988"
     CDS             10418..10852
                     /gene="phnO"
                     /locus_tag="IT904_001988"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAP3191772.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            10854..11612
                     /gene="phnP"
                     /locus_tag="IT904_001989"
     CDS             10854..11612
                     /gene="phnP"
                     /locus_tag="IT904_001989"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAP3191773.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(11759..12088)
                     /gene="yjdP"
                     /locus_tag="IT904_001990"
     CDS             complement(11759..12088)
                     /gene="yjdP"
                     /locus_tag="IT904_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAP3191774.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12157..12606)
                     /gene="rpiB"
                     /locus_tag="IT904_001991"
     CDS             complement(12157..12606)
                     /gene="rpiB"
                     /locus_tag="IT904_001991"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HAP3191775.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            12965..13855
                     /gene="alsR"
                     /locus_tag="IT904_001992"
                     /gene_synonym="rpiR"
     CDS             12965..13855
                     /gene="alsR"
                     /locus_tag="IT904_001992"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HAP3191776.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            13914..14849
                     /locus_tag="IT904_001993"
     CDS             13914..14849
                     /locus_tag="IT904_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose-binding periplasmic protein"
                     /protein_id="HAP3191777.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            14976..16508
                     /gene="alsA"
                     /locus_tag="IT904_001994"
     CDS             14976..16508
                     /gene="alsA"
                     /locus_tag="IT904_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HAP3191778.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IDRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGIVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDQRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16487..17467
                     /gene="alsC"
                     /locus_tag="IT904_001995"
     CDS             16487..17467
                     /gene="alsC"
                     /locus_tag="IT904_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HAP3191779.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17478..18173
                     /locus_tag="IT904_001996"
     CDS             17478..18173
                     /locus_tag="IT904_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HAP3191780.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18157..19086
                     /gene="alsK"
                     /locus_tag="IT904_001997"
     CDS             18157..19086
                     /gene="alsK"
                     /locus_tag="IT904_001997"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HAP3191781.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLRDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLVAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQFCAKAP"
     gene            19359..21344
                     /gene="yjcS"
                     /locus_tag="IT904_001998"
     CDS             19359..21344
                     /gene="yjcS"
                     /locus_tag="IT904_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAP3191782.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMGNGDNLNLTLNDSVLNYRKTLQPQANASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            21553..21828
                     /locus_tag="IT904_001999"
     CDS             21553..21828
                     /locus_tag="IT904_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191783.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCTIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            21847..22878
                     /gene="mdtN"
                     /locus_tag="IT904_002000"
     CDS             21847..22878
                     /gene="mdtN"
                     /locus_tag="IT904_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAP3191784.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWHVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            22878..>23352
                     /locus_tag="IT904_002001"
     CDS             22878..>23352
                     /locus_tag="IT904_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191785.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLW"
BASE COUNT         5440 a         6520 c         6274 g         5118 t
ORIGIN      
        1 ccgcatccgg caacggtgcc gactgcctga tgcgacgctc gcgcgtctta tcaggcctac
       61 aaattcccgc accctccgta ggccggataa ggcgtttacg ccgcatccgg caacagtgcc
      121 gactgcctga tgcgacgctg accgcgtctt atcaggcttc cctctcatct gtataaattt
      181 cgaactacac ttaactggct tctcttaact gaggtcacca tcatgccgtt aagtccctac
      241 ctctcttttg ccggtaactg tgccgacgca attgcttatt atcaacgtac gttgggcgct
      301 gaactgctct ataaaatcag cttcggcgaa atgccgaaat cagcgcagga cagcgccgag
      361 aactgccctt ccggaatgca atttcccgat accgccatcg cccacgccaa tgtgcgcatt
      421 gccggaagcg atatcatgat gagcgatgct ataccgtcag gaaaagccag ctactccggc
      481 tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa aacgctggtt tgataacctt
      541 gccgctaacg gaaaaatcga aatggcctgg caggaaactt tctgggcgca tggcttcggc
      601 aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg
      661 caataatccg ccgggaggcc cgccctcccg cactgtcatc gaattcccgt taactcttca
      721 tctgttagtc acttttaatt aaccaaatcg tcacaataat ccgccacgat ggagccactt
      781 ttttagggag gctgcatcat gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc
      841 aatcagcatc aggcgctgca tgcggttgat ctgaacattc atcacggtga aatggtggct
      901 ctgcttgggc cgtcgggttc cggcaaatcc acccttttac gtcacttaag cggtttgatt
      961 accggcgata aatccgccgg cagccatatc gagctgctgg gccgcacagt ccagcgcgaa
     1021 ggccgtctgg cgcgcgatat ccgcaaaagc cgcgccaaca ccggctacat cttccaacaa
     1081 ttcaacctgg tgaaccgcct gagcgtactg gagaacgtgc tgattggcgc gctcggcagc
     1141 acgccgttct ggcgcacctg ttttagctgg tttacccgcg agcagaaaca acgcgcgtta
     1201 caggcgctga cccgcgttgg catggtgcat tttgcccatc aacgcgtttc caccctctcc
     1261 ggcggacagc agcagcgtgt ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt
     1321 ctggccgatg aacccatcgc ctcgctggac ccggaatccg cccgcatcgt gatggacacc
     1381 ctgcgcgaca tcaatcagaa cgacggcatc accgtggtcg tcacgctgca tcaggtggat
     1441 tacgccctgc gctactgcga acgcatcgtc gccctgcgcc aggggcacgt tttctacgac
     1501 ggcagcagcc aacagtttga taacgaacgt tttgaccatc tctaccgcag cattaatcgc
     1561 atcgaagaga acgcgaaagc tgcctgacat ccccatcatt gaggaaaacg aatgaacgct
     1621 aagataattg cctcgctggc cttcaccagc atgttcagcc tcagcaccct gttaagcccg
     1681 gcacacgccg aagagcagga aaaggcgctg aatttcggca ttatttcaac ggaatcacag
     1741 caaaacctga aaccgcaatg gacgccattc ttacaggata tggagaagaa gctgggcgtg
     1801 aaggtgaacg ccttctttgc cccagactac gcaggcatta tccagggaat gcgcttcaat
     1861 aaagtggata tcgcctggta cggcaacctg tcggcaatgg aagcggtgga tcgcgccaac
     1921 ggccaggtct tcgctcagac cgtcgcggcg gacggatcgc cgggttactg gagcgtgttg
     1981 atcgtcaaca aagatagtcc gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat
     2041 ctcaccttcg gcaatggcga tcctaactcc acctctggct tcctcgtccc cggttactac
     2101 gtctttgcca aaaacaatat ctccgccagc gacttcaagc gcaccgtcaa cgccgggcat
     2161 gaaaccaacg cgctggccgt cgccaacaag caggtggatg ttgccaccaa caacaccgaa
     2221 aacctcgaca agctgaaaac ctccgcgcca gagaagctga aagaactgaa ggtgatctgg
     2281 aagtcgccgc tgatcccagg cgatccgatc gtctggcgca agaatctttc cgaaaccacc
     2341 aaagacaaga tctacgactt ctttatgaac tacggcaaaa cgccggaaga aaaagcggtg
     2401 ctggaacgcc tgggctgggc gccattccgc gcttccagcg acctgcaact ggtgccgatt
     2461 cgccagctcg cgctgtttaa agagatgcag ggcgtgaaaa gcaataaagg actgaatgag
     2521 caggacaagc tggcaaaaac caccgagatt caggcgcagc tggatgacct ggaccgcctg
     2581 aacaacgcgc taagcgcgat gagttcggtg agtaaagcgg tgcagtaaat cgtaggtcgg
     2641 ataagacgcc ccggcgtcgc atccgacaat gtgcaggcgt tgatgccgga tgcggtgcaa
     2701 gcaccttatc cggcctacag accggagcca aacatgcaaa ccatcaccat cgccccaccc
     2761 aagcgcagct ggttctcgct tctgagctgg gccgttgttc tcgccgtgct ggtcgtctcg
     2821 tggcagggcg cggaaatggc cccgctcacg ctgattaaag acggcggcaa catggcaacc
     2881 ttcgctgccg acttcttccc gcccgatttc agccagtggc aggattactt caccgaaatg
     2941 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cggtggttct ctccatcccc
     3001 tttggcctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc
     3061 ctgatggacg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3121 gtcggtctcg gaccgttcgc tggcgtgctg gcgctattta tccacaccac cggcgtgctc
     3181 tccaagctgc tttccgaagc ggtagaagca attgaacctg gcccggtgga aggcattcgc
     3241 gccaccggtg ccaacaagct cgaagagatc ctctacggcg tgctgccaca ggtgatgcca
     3301 ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3361 ggcatggtcg gcgcaggcgg gatcggcgtc accctgtggg aagcgattcg cggtttccag
     3421 ttccaacaaa cctgcgccct gatggtgctt atcatcgtca cggtcagcct gctggatttc
     3481 ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgata tctatgcact
     3541 tgtctacaca tccgaccagc tacccaacac gctatcaaga gatagccgca aaacttgagc
     3601 aggagcttcg tcaacactac cgctgcggcg actatcttcc cgccgagcag caactggcag
     3661 cgcgctttga ggtgaatcgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     3721 gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc
     3781 cgctcaacgc ccaggcgcgt tttagccaga atctgctgga tcagggcagc catcccacca
     3841 gcgaaaaact gctttcggta ttgcgccccg cgtccggcca cgtcgctgac gcactgggga
     3901 ttaccgaggg ggagaacgtc atccacctgc gcaccctgcg tcgtgtcaac ggcgtcgcgc
     3961 tctgtttaat cgaccactac ttcgcggacc tcaccctctg gccgacgctg caacgcttcg
     4021 acagcggctc gctgcacgat tttctgcgcg agcaaaccgg aattgcgctg cgccgcagcc
     4081 agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcgtctt gaaatcccga
     4141 atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg
     4201 cggagtactc cgtcagcctg acgcgcgccg acatgattga attcactatg gagcactgaa
     4261 tgcacgcaga taccgcgacc cgccagcact ggatgtccgt gctggcgcac agccaaccgg
     4321 ctgaactggc agcacgcctg aacgcgctaa acatcaccgc cgactatgag gtgatccgcg
     4381 ccgctgaaac tggcctggta cagattcagg cgcggatggg cggcaccggc gaacgttttt
     4441 ttgccggcga cgccacgctg acccgcgccg ccgtgcgcct gactgacggc acgctcggct
     4501 acagctgggt gcaggggcgt gataaacagc acgccgaacg ctgcgcgctg attgacgcgc
     4561 tgatgcagca atctcgccac tttcaaaact tatcagaaac ccttattgcc ccgctggacg
     4621 ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt gaacgccagc cgggtcgact
     4681 tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta tgcttcccgt
     4741 gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat
     4801 tgtcgccctg catcagctca aacgcggctg gcaaccgctg aatatcgcca ccaccagcgt
     4861 gctgctgacg ctggccgata acgacacgcc ggtgtggctt tctaccccat taaataacga
     4921 tatcgtcaac cagagcctgc gttttcatac caacgcgccg ctggtcagcc agccggaaca
     4981 ggcgaccttc gcggtgacgg atgaggcgat ttccagcgaa cagctcaacg ccctttccac
     5041 cggcaccgcc gttgcgccgg aagcgggtgc gacgctgatt ttacaggtct ccagcctgag
     5101 cggcggacgc atgttgcgcc ttactggtgc gggtattgcc gaagaacgaa tgatcgctcc
     5161 gcagctgccg gagtgcattc tgcacgaact caccgagcgc ccgcatccgt tcccgctcgg
     5221 catcgacctg atcctgacct gtggcgagcg cctgctggct attccgcgaa ccactcatgt
     5281 ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaga aggcgatcga cgccgcccac
     5341 gccctgcaag agagccgacg ccgaggcgat accgatttgc ccgaactgag cgtcgcccag
     5401 attgaacagc agcttaacct cgcggtagat cgcgtgatga ccgaaggcgg cattgccgac
     5461 cgcgaactgg cggcgctggc gctgaaacag gccagcggcg ataacgttga agcgattttc
     5521 ctgctgcgcg cctaccgcac cacgttggcg aagctggcgg taagcgagcc gctcgacacc
     5581 accgggatgc gtctcgaacg ccgtatctcc gccgtttata aagacattcc cggcggccag
     5641 ctgcttggcc caacctacga ctacacccat cgcctgctcg attttaccct gctggcaaac
     5701 ggcgaagcgc cgacgctgac caccgccgac agcgaacagc agccgtcgcc gcacgttttc
     5761 agcctgctgg cgcgtcaggg gctggcgaag tttgaagagg atagcggcgc acagccggat
     5821 gacatcaccc gcacgccgcc ggtttacccc tgctcacgct cctcccgttt gcagcagttg
     5881 atgcgcggcg acgaaggcta tttgctggcg ctggcctact ccacccaacg cggttacggg
     5941 cgcaatcacc cgttcgcagg cgagatccgc agcggctata tcgacgtgtc gattgtgccg
     6001 gaagagctgg gatttgcggt gaacgtcggc gaactgctga tgactgagtg tgaaatggtt
     6061 aacggtttta tcgacccgcc gggtgagccg ccgcacttca cgcgcggcta cgggctggtg
     6121 ttcggcatga gcgagcgcaa agcgatggcg atggcgctgg tcgaccgcgc tctgcaagcc
     6181 ccggagtacg gcgagcacgc gacaggcccg gcgcaggatg aagagttcgt gctggcacat
     6241 gccgacaacg tcgaagccgc aggctttgtc tcacacctca aactccccca ctacgtcgat
     6301 ttccaggccg aactggagct actcaaacgt ctgcaacagg agcagaacca tggctaatct
     6361 gagcggctac aactttgcct acctcgacga gcagaccaaa cgcatgatcc gccgcgccat
     6421 cttaaaagcg gtggcgatcc ccggttatca ggtgccgttt ggcgggcgcg agatgccgat
     6481 gccgtacggc tggggaaccg gcggcattca gcttaccgcc agcgtgattg gcgaaagcga
     6541 cgtgctgaag gtgattgacc agggcacgga tgacaccacc aacgccgtgt cgattcgcaa
     6601 cttcttcaag cgcgtgaccg gggtaaacac cacggaacgt acggacgatg cgacggttat
     6661 ccagacgcgt caccgcatcc ccgaaacgcc gctgaccgaa gatcagataa ttatcttcca
     6721 ggtgccaatc cccgagccgc tgcgctttat cgagccgcgc gaaacggaaa cccgcaccat
     6781 gcacgcgctg gaagagtacg gcgtgatgca ggtgaaactg tatgaagata tcgcccgctt
     6841 cggtcatatc gccaccacct acgcctatcc ggtgaaggta aatgggcgct acgtgatgga
     6901 cccgtcgccg atcccgaaat tcgataaccc aaaaatggac atgatgcccg ccctgcaact
     6961 gttcggcgcg gggcgcgaga agcgcatcta tgcggtgccg ccgtttaccc gcgtggaaag
     7021 tctcgatttc gacgatcacc cgttcaccgt tcagcagtgg gatgagccat gcgccatctg
     7081 cggatcgacc cacagctatc ttgatgaagt ggtgctggat gacgccggaa accgcatgtt
     7141 tgtctgctcc gataccgatt attgccgcca acagagcgag gcaaaaaacc aatgaatcaa
     7201 ccgttacttt cggtcaataa cctgacccac ctttacgcgc cgggcaaagg ctttagcgat
     7261 gtctcttttg atttatggcc gggggaagtg ctgggcattg tcggggaatc cggctccggg
     7321 aagaccacgc tgctgaagtc gatctccgcg cgcctgacgc cgcagcaggg ggaaattcac
     7381 tacgagaacc gttcgctgta tgcaatgagc gaggccgacc gccgtcgcct gctgcgtacc
     7441 gaatggggcg tggtgcatca gcatccactc gacggcctgc gccgccaggt gtcggcaggc
     7501 ggcaatatcg gcgagcggct gatggcgacc ggggcacgtc attacggcga tattcgtgcc
     7561 accgcgcaga agtggctgga agaggtggag attcccgcca accggatcga cgacctgccg
     7621 accacctttt ccggcggtat gcagcagcgt ttgcagattg cccgcaacct ggtgacgcat
     7681 ccgaagctgg tgtttatgga tgaaccgacc ggcgggctgg atgtgtcggt gcaggcccgc
     7741 ctgctcgacc tgctgcgcgg cctggtggtg gagctgaacc tcgcggtggt gattgtcacc
     7801 catgatttag gcgtcgcccg cctgctggcg gaccgtttgc tggtgatgaa gcaggggcaa
     7861 gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc cgcatcatcc gtatacacag
     7921 ctgctggtgt catcggtttt gcagaattga gccggtgccg gatgcggcgt aaacgcctta
     7981 tccggcctac aaatgcgctc cccgtaggtc ggataagacg cgtcagcgtc gcatccgaca
     8041 cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc
     8101 accagcaaaa cggcgtgcgc ctgcccgtcc tcaatcgcgc ctcgctcacc gtcaacgcgg
     8161 gcgaatgcgt ggtgctccac ggccattccg gcagcggcaa atcaactctg ctacgctcgc
     8221 tgtacgccaa ctatctaccc gacgaaggtc aaatccagat caaacacggt gacgagtggg
     8281 tagacctggt caccgcgcca gcgcgcaaag tggtggaaat ccgcaaaacc accgtcggct
     8341 gggtgagcca gtttctgcgc gtcatcccgc gtatctcagc actggaagtg gtgatgcagc
     8401 cgctgctcga taccggcgtt ccgcgtgaag cctgcgccgc taaagccgcg cgtcttctca
     8461 cccgcctgaa cgtgccggaa cgcctgtggc acctggcacc atcgacattt tccggtggcg
     8521 aacagcagcg cgtcaacatc gcccgcggct ttatcgtcga ctaccccatt ctgctgcttg
     8581 acgaacctac cgcctcgctg gacgccaaaa acagcgccgc ggtggtggaa ctgattcgcg
     8641 aagccaaaac ccgtggcgca gccatcgtag gcatcttcca tgacgaagct gtacgtaatg
     8701 acgtcgccga ccgcctgcac ccaatgggag cctcttcatg attatcaata acgttaagct
     8761 ggtgctggaa aacgaggtgg taagcggttc gctggaggtg cagaacggcg aaatccgcgc
     8821 ctttgccgaa agccagagcc gcctgccgga ggcgatggac ggcgaaggcg gctggctgct
     8881 gccggggctg attgagctgc ataccgataa tctggataaa ttcttcaccc cgcgcccgaa
     8941 agttgactgg cctgcccact cggcgatgag cagccacgac gcgctgatgg tggcgagcgg
     9001 catcaccacc gtactggatg ccgtggcaat tggcgacgtg cgcgacggcg gcgatcggct
     9061 ggagaatctg gagaagatga tcaacgccat cgaagagacg cagaaacgcg gcgtcaaccg
     9121 cgccgagcac cgtctgcatc tgcgctgcga actgccgcat cacaccacgc tgccgctgtt
     9181 tgaaaaactg gtgcagcgcg agccggtgac gctggtgtcg ctgatggacc actcgccggg
     9241 ccagcgccag ttcgccaacc gcgagaagta tcgcgaatat tatcagggca aatactccct
     9301 cactgatgcg cagatgcagc agtacgaaga agagcaactg gcgctcgccg cacgctggtc
     9361 gcagccgaat cgcgaatcca tcgccgccct gtgccgcgcg cgaaaaattg cgcttgccag
     9421 ccacgatgac gccacccacg cccacgttgc tgaatctcac cagcttggca gcgtgatcgc
     9481 cgaatttccc accacgttcg aagcggcgga agcctcgcgc aagcatggca tgaacgtgct
     9541 gatgggcgcg ccgaatattg tgcgcggcgg ctcgcactcc ggcaacgtgg cggccagtga
     9601 actggcgcag cttggcctgc tggatatcct ctcttccgac tactaccccg ccagcctgct
     9661 cgatgcggca tttcgcgtcg ccgatgacga gagcaaccgc tttacgctgc cgcaggcggt
     9721 gaagctggtg actaaaaatc cagcgcaggc gcttaatctc caggatcgcg gggtgattgg
     9781 cgagggcaaa cgcgccgacc tggtgctggc gcatcgcaag gacaatcata ttcatatcga
     9841 ccacgtctgg cgtcagggta aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg
     9901 gggccgtccg gctccgggaa agacagcctg ctggcggaac tccgcctgcg ggaacaaact
     9961 cagttactgg tggcgcatcg ctacatcacg cgcgatgcca gcgccggaag tgaaaaccat
    10021 atcgccctga gcgagcagga gttttttacc cgcgcggggc aaaatctgtt ggccttaagc
    10081 tggcacgcta acggtctgta ttatggcgtc ggcgtcgaga ttgatctctg gctgcacgcc
    10141 ggattcgacg tgctggtcaa cggctcacgc gcccatctgc cgcaggcgcg ggcgcgctat
    10201 caatcggcgc tgctgcccgt ctgtttacag gtttcgccgg agatcctccg ccagcgcctg
    10261 gaaaaccgtg gccgtgaaaa cgccagtgaa attaacgccc gcctggcgcg cgccgcccgc
    10321 tatactccac aggattgcca tacgctcaac aatgacggca gcctgcgcca gtcggtcgac
    10381 acgctgctga cgctgatcca tcagaaggag aaacaccatg cctgcttgtg agcttcgccc
    10441 ggccacgcag tacgacaccg acgcggttta cgcgctgatt tgtgagctaa aacaggcgga
    10501 gtttgaccac cacgcgtttc gcgtgggttt taacgccaat ctgcgcgacc caaacatgcg
    10561 ctaccatctg gcgctgcttg atggcgaagt tgtcggcatg atcggcctgc atttgcagtt
    10621 tcatctgcat catgtcaact ggatcggcga aattcaggag ttggtggtaa tgccgcaggc
    10681 gcgcggtctg aacgtcggca gtaagttact ggcgtgggca gaagaagaag cccgccaggc
    10741 cggggccgaa atgaccgaac tttcgaccaa cgtgaagcgc cacgacgcgc accgtttcta
    10801 tctgcgcgaa ggctacgagc agagccactt ccgcttcacc aaggcgctgt aacatgagcc
    10861 tgaccctcac gctcaccggc accggcggcg cacagggcgt tccggcatgg ggctgcgagt
    10921 gtgcggcctg cgccagagcg cggcgctcgc cgcagtatcg ccgccaaccg tgcagcggcg
    10981 tagtgaagtt taacgacgca atcaccctga tcgacgccgg gctgcacgat ctcgccgatc
    11041 gctggtcgcc cggatcgttc cagcagtttt tgctgacgca ttatcatatg gatcacgtcc
    11101 aggggctgtt tccgctgcgc tggggcgttg gcgatccgat cccggtttac ggcccgccgg
    11161 atgaacaggg ctgcgacgat ctgtttaaac atccgggcct gcttgatttc agccacacgg
    11221 tggaaccgtt tgtggtgttt gatttgcagg ggttacaggt cacgcccctg ccgctcaacc
    11281 actcaaaact gaccttcggt tatctgctgg aaacggcaca cagccgggtg gcgtggctgt
    11341 ctgacaccgc aggcttgccg gaaaaaacgc tgaaattttt acgcaataat cagccgcagg
    11401 taatggtgat ggattgcagt cacccgccgc gcgcggatgc accgcgtaat cactgtgatt
    11461 taaataccgt gcttgcgctg aatcaggtta tccgctcgcc acgggtgatt ctgacccata
    11521 tcagccacca gtttgatgcg tggctgatgg aaaacgcact accgtcaggg tttgaggtgg
    11581 ggtttgatgg gatggagatt ggggtggcgt gatgagaggg aatgtgcgcg ctggccccct
    11641 caccctaacc ctctccccag aggggcgagg ggaccgattg tgctcgatat tgaatattgc
    11701 gctcgttttc tccctctccc cattggggtg aggggcgatg cctgctccat acccaacctc
    11761 atcgcccata ctcatcttcc attctccgct cttcatcctc cagttgccga cgctcctgat
    11821 caagctggcg ctggcgatcg tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt
    11881 cgtcatattg tctgcgccga tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat
    11941 cttcatagtc tcgcccacgg tcagggttat aagcgtcatt aatcgcctgc tgaatattgc
    12001 caatggtgtc gtcgataata tcggcctggg ccggaacggt ggacagcgtg agcagggtga
    12061 ataaaagaaa tagcggaaag cgtttcatta gccaacctca aaaagaaact ctatccacat
    12121 taatcattac tcatccatgc aagtagtgga tgaatctcaa tttctccgct gctctattgc
    12181 cgtaatcgcc tccacgcgtt gttgatgacg accgccttcg tactgtgcgc ccagccacgc
    12241 atccacaatc atttttgcca gttcgaggcc aaccactcgt gaaccaaaag ccagcacgtt
    12301 ggtgtcgtta tgctgccgcg aaagttgcgc ggaataaggt tcgctacaga cgaccgcgcg
    12361 aattccggca aacttgttcg ccgctatcga aataccgacg ccagtaccac aaatcaaaat
    12421 cccgccatca acctctccgc cagcaacagc cagtgcgact tgactggcgt aatgtggata
    12481 atcagtacgc tctgacgacc aggttccttt atcaatcact tcaacgccac gctcaactaa
    12541 atgtgccact atttcatgtt ttaaaatgaa accgacatga tcacagccaa atgcaatctt
    12601 tttcattcat cttccccgtc cgtgcaaaac ttcacaatca aaaaacattt gtgatgttaa
    12661 tgaattaaaa acacccaaaa tcgatgaatt acgccttcct gatctgtttt acagagttac
    12721 atctgacagc gcaatagcgt taaagacact caccatccag caacatctat catctaaaaa
    12781 accagaaaaa caaataacat catgttttta aactaattaa atgaaataaa attttaagcc
    12841 actcgccatt gttcacaata aaataaactt tataaatttt atttttttgt gaagtcgcca
    12901 gcatcttttc tgttcttgct gtggtgatat agtggcgtct tcaattcaag gacaagagaa
    12961 cgtgatgagc cagtcagagt ttgattcagc gcttccgaac ggtatagggt tagcgcctta
    13021 cctgcgaatg aagcaggaag gaatgacaga aaatgaaagc cgcatcgtgg agtggttact
    13081 caaacccggt aacctgagtt gtgcacccgc aattaaagat gtcgcagaag ctctggcggt
    13141 atctgaagcg atgatagtta aggtatcaaa gctgctgggg tttagcggct ttcgtaactt
    13201 acgcagtgcg ctggaagatt atttttctca gtcagaacag gtattgcctt ccgagttggc
    13261 ttttgatgaa gcgccgcagg atgtggtgaa taaggtattt aacatcactt tacgcaccat
    13321 tatggaaggt cagtcgatcg tcaacgttga tgagatccac cgtgccgccc gctttttcta
    13381 tcaggccaga cagcgggatt tgtacggtgc cggaggatca aatgctatct gtgctgatgt
    13441 acagcacaag ttcttgcgca ttggcgtacg ctgtcaggcc tatcctgatg ctcacatcat
    13501 gatgatgtcc gcttcgttgt tacaggaagg agatgttgtg ctggtagtga cccattccgg
    13561 gcgaaccagt gatgtaaaag cggccgtaga actggcaaaa aagaacgggg caaagattat
    13621 ttgtataacc catagctacc attcaccgat agcgaaactg gccgattata ttatttgctc
    13681 accagccccg gaaacgccgt tattaggtcg taatgcctcg gcaagaatat tacaactaac
    13741 tttgctggac gctttttttg tctctgtcgc ccagctcaac attgaacaag ctaatattaa
    13801 tatgcaaaaa accggcgcaa ttgttgattt cttctcacca ggcgcgctga aataagaaaa
    13861 aggtaattcc gccatcgcgg agttacctgt ccccttaccg tgagaacatt attatgaata
    13921 aatatctgaa atatttcagc ggcacactcg tgggcttaat gttgtcaacc agcgcttttg
    13981 ctgccgccga atatgctgtc gtattgaaaa ccctctccaa cccattttgg gtagatatga
    14041 aaaaaggcat tgaagatgaa gcaaaaacac tgggcgtcag cgttgatatt tttgcctctc
    14101 cttcagaagg cgattttcaa tctcaattgc agttatttga agatctcagt aataaaaatt
    14161 acaaaggtat cgccttcgct ccattatcct cagtgaatct ggtcatgcct gtcgcccgcg
    14221 catggaaaaa aggcatttat ctggttaatc tcgatgaaaa aatcgacatg gataatctga
    14281 aaaaagctgg cggcaatgtg gaagcttttg tcaccaccga taacgttgct gtcggggcga
    14341 aaggcgcgtc gttcattatt gacaaattgg gcgctgaagg tggtgaagtc gcaatcattg
    14401 agggtaaagc cggtaacgcc tccggtgaag cgcgtcgtaa tggtgccacc gaagccttca
    14461 aaaaagcaag ccagatcaag cttgtcgcca gccagcctgc cgactgggac cgcattaaag
    14521 cactggatgt cgccactaac gtgttgcaac gtaatccgaa tattaaagcg atctattgcg
    14581 cgaatgacac gatggcaatg ggtgttgctc aggcagtcgc aaacgccgga aaaacgggaa
    14641 aagtgctggt cgtcggtaca gatggcattc cggaagcccg caaaatggtg gaagccggac
    14701 aaatgaccgc gacggttgcc cagaacccgg cggatatcgg cgcaacgggt ctgaagctga
    14761 tggttgacgc tgagaaatcc ggcaaggtta tcccgttgga taaagcaccg gaatttaaac
    14821 tggtcgattc aatcctggtc actcaataaa actgaatgat gccggatgat gatgctaatg
    14881 aagtgtctta tccggccaac aaaataacct gaacgtagac ctgataaatg tagccatcag
    14941 gcaataatac gatatttcgt gcagagaggt tatttatggc cacgccatat atatcgatgg
    15001 cggggatcgg caagtccttt ggtccggttc acgcattaaa gtcggttaat ttaacggttt
    15061 atcctggtga aatacatgca ttactaggag aaaatggcgc gggtaaatcc acgctaatga
    15121 aagttttatc cggaatacat gagccgacca aaggcaccat taccattaat aacattagct
    15181 ataacaagct ggatcataaa ttagcggcac aactcggtat cgggattatt tatcaggaac
    15241 tcagcgttat tgatgaatta accgtactgg aaaatttata tattgatcgt catctgacga
    15301 aaaaaatctg tggcgtcaat attatcgact ggcgagaaat gcgtgtccgc gccgccatga
    15361 tgttattacg cgtgggcttg aaagttgatc tagatgagaa agtggcgaat ttatctatca
    15421 gccacaagca gatgctagaa attgccaaaa cgctgatgct cgatgccaaa gtcatcatca
    15481 tggatgaacc cacctcctca ctcaccaata aagaggtgga ctatctgttt ctgatcatga
    15541 atcagttgcg taaagagggt acggccatcg tctatatctc gcataagttg gcggaaattc
    15601 gccgtatttg cgaccgctat acggtgatga aagacggcag cagcgtttgc agcggcatag
    15661 taagcgatgt gtcaaatgac gatatcgtcc gtctgatggt aggccgcgaa ctgcaaaacc
    15721 gttttaacgc gatgaaggag aatgtcagca accttgcgca cgaaacggtt tttgaggtgc
    15781 ggaacgtcac cagtcgtgac agaaaaaagg tccgggatat ctcatttagc gtctgccggg
    15841 gagaaatatt aggctttgcc ggactggtcg gttccggacg tactgaactg atgaattgtc
    15901 tgtttggcgt ggatcaacgc gctggcggag aaatccgtct taatggcaaa gatatctctc
    15961 cacgttcacc cctggatgcc gtgaaaaaag ggatggctta catcactgaa agccgccggg
    16021 ataacggttt tttccccaac ttttccatcg ctcagaacat ggcgatcagc cgcagtctga
    16081 aagacggcgg ctataaaggc gcgatgggct tgtttcatga agttgacgag caacgtaccg
    16141 ctgaaaatca acgcgaactg ctggcgctga aatgtcattc ggtaaaccag aatatcaccg
    16201 aactctccgg gggaaatcag cagaaagtcc tgatctccaa atggctgtgc tgttgcccgg
    16261 aagtgattat tttcgatgaa cctacccgcg gcatcgacgt tggcgcgaaa gccgaaattt
    16321 acaaagtgat gcgccaactg gcggacgacg gaaaagtcat cctgatggtg tcatctgaac
    16381 tacctgaaat tatcaccgtc tgcgaccgca tcgccgtgtt ctgcgaagga cgactgacgc
    16441 aaatcctgac gaatcgcgat gacatgagcg aagaggagat tatggcatgg gctttaccac
    16501 aagagtaaaa agcgaagcga gcgagaagaa accgttcaac tttgcgctgt tctgggataa
    16561 atacggcacc ttttttatcc tggcgatcat cgtcgccatc tttggttcgc tgtcaccaga
    16621 atattttctg accaccaata atattaccca gatttttgtt caaagctccg tgacggtatt
    16681 gatcggcatg ggcgagtttt tcgctatcct ggtcgctggt atcgacctct cggttggcgc
    16741 gattctggcg ctttccggta tggtgaccgc caaactgatg ttggcaggtg ttgacccgtt
    16801 tctcgcagcg atgattggcg gtgtactggt tggcggcgca ctgggggcga tcaacggctg
    16861 cctggtcaac tggacggggc tacacccgtt catcatcacc cttggcacca acgcgatttt
    16921 ccgtgggatc acgctggtga tctccgatgc caactcggta tacggcttct catttgactt
    16981 cgtgaacttc tttgccgcca gcgtaattgg gatacctgtc cccgttatct tctcactaat
    17041 tgtcgcgctc atcctttggt ttctgacaac gcgtatgcgg ctcgggcgca acatctacgc
    17101 actgggcggc aacaaaaatt cggcgttcta ttccgggatt gacgtgaaat tccacatcct
    17161 ggtggtgttt atcatctccg gtgtttgtgc aggtctggca ggcgtcgtct caactgcacg
    17221 actcggtgcc gcagaaccgc ttgccggtat gggttttgaa acctatgcca ttgccagcgc
    17281 catcattggc ggcaccagtt tcttcggcgg caaggggcgc attttctctg tggtgattgg
    17341 cgggttgatc atcggcacca tcaacaacgg tctgaatatt ttgcaggtac aaacctatta
    17401 ccaactggtg gtgatgggcg gattaattat cgcggctgtc gcccttgacc gtcttatcag
    17461 taagtaagga attgaacatg aaaatctccc cctcgttaat gtgtatggat ctgctgaaat
    17521 ttaaagaaca gatcgaattt atcgacagcc atgccgatta cttccacatc gatatcatgg
    17581 acggtcactt tgtccccaat ctgacactct caccgttctt cgtaagtcag gttaaaaaac
    17641 tggcaactaa accgctcgac tgtcatctga tggtgacgcg gccgcaggat tacattgctc
    17701 aactggcgcg tgcgggagca gatttcatca ctctgcatcc ggaaaccatc aacggccagg
    17761 cgttccgcct gattgatgaa atccgccgtc atgacatgaa agtggggctg atccttaacc
    17821 cggagacgcc agttgaggcc atgaaatact atatccataa ggccgataaa attacggtca
    17881 tgactgtcga tcccggcttt gccggacaac cgttcattcc tgaaatgctg gataaacttg
    17941 ccgaactgaa ggcatggcgt gaacgagaag gtctggagta cgaaattgag gtggacggtt
    18001 cctgcaacca ggcaacttac gaaaaactga tggcggcagg ggcggatgtc tttatcgtcg
    18061 gcacttccgg cctgtttaat catgcggaaa atatcgacga agcatggaga attatgaccg
    18121 cgcagattct ggctgcaaaa agcgaggtac agcctcatgc aaaaacagca taacgtcgta
    18181 gcgggcgtgg atatgggggc aacgcatatc cgcttttgtc tgcggacagc agaaggtgaa
    18241 acgctacact gcgaaaaaaa gcggaccgca gaagtcattg ctcccggcct ggtgtcgggt
    18301 atcggcgaaa tgattgacga gcaactcagg cgctttaacg ctcgctgtca tggtctggtg
    18361 atgggatttc cggcgctggt cagtaaagat aaacgcacca ttatttctac gcctaacctg
    18421 ccgttaacag cggcggattt atatgatctc gccgataagc tcgaaaatac gctgaattgt
    18481 ccggttgagt tttcccgcga cgttaacctg caactctcct gggacgtagt agaaaaccgc
    18541 cttacgcaac aactggttct ggcggcctat ctcggtacgg ggatggggtt cgctgtgtgg
    18601 atgaacggtg cgccgtggac gggggcacac ggtgtggcag gcgaactggg tcatatccct
    18661 ctgggagata tgacccaaca ctgcgcgtgt ggcaatcctg ggtgcctgga aaccaattgc
    18721 tctggaatgg cgctaagacg ctggtacgaa caacagcccc gaaattaccc attgcgcgat
    18781 cttttcgtcc atgcggaaaa cgcccctttc gtccagagtc tgcttgaaaa cgcggcacgg
    18841 gccattgcca ccagcattaa tctgttcgat cccgatgcgg tgatcctggg cggtggcgtg
    18901 atggatatgc ccgccttccc acgcgagact ctcgttgcca tgacccaaaa gtacctgcgc
    18961 cgtccactgc cgcatcaggt cgtgcgcttt attgccgcct catcttctga ctttaatggc
    19021 gctcagggtg cagcaatatt ggcgcatcaa cgttttttgc cacagttctg tgctaaagcc
    19081 ccatgagtaa aacgttggca cagtagcgca atgtcccgct gtgccaacga caggtacaat
    19141 atatataata aacacataaa acgactcata ttattttctg caatgatagt tttactgtaa
    19201 ttttccctct tcagcacaaa tgcctaataa aatggcgcgt tccgctcaca cttccactta
    19261 ttaaagtcgc aatttcccaa ttatgataac gccagcttta aatgacgtat tttgcatgga
    19321 ttacatattt acgacataaa tgataaatgg agatttatat gaataactct cggttattcc
    19381 gtttgagcag gattgttatt gcgttaactg ccgccagcgg catgatggta aataccgcta
    19441 acgcgaaaga ggaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg
    19501 ccaaatcatt accgtttagc gatcgtcagg attttgacga tgcccagcgt ggatttatcg
    19561 ccccgctgct ggatgaaggt attctgcgtg atgcgaacgg taaagtttac taccgcgcgg
    19621 acgattacaa atttgatatt aatgccgcag cgccggaaac cgtaaacccc agcctgtggc
    19681 gtcagtcgca aatcaacggt atttctggcc tgttcaaagt caccgataaa atgtatcagg
    19741 tgcgcggcca ggatatctct aacattacgt tcgttgaggg cgagaaaggc attattgtta
    19801 tcgacccgct ggtgacgccg cctgccgcaa aagccgcact tgacctttac ttccagcatc
    19861 gtccgcaaaa accgattgtt gccgttatct acactcacag ccacaccgac cactatggtg
    19921 gcgtgaaagg cattatctct gaagccgatg ttaaatccgg caaagttcag gtgattgccc
    19981 ctgcaggctt tatggacgaa gccatcagcg aaaacgtgct ggcgggtaac atcatgagcc
    20041 gccgtgcgct ctactcttac ggtctgttac tgccgcacaa cgcgcaaggc aacgtgggta
    20101 atggccttgg cgtgacgctg gcaacgggcg acccgagcat tattgcaccg actaaaacta
    20161 tcgtccgaac tggcgagaag atgattatcg acggcctgga gtttgacttc ctgatgaccc
    20221 caggtagcga agcgccagcc gaaatgcact tctatattcc ggccctgaaa gccctgtgta
    20281 ccgccgagaa cgccacgcat accctgcaca acttctacac tctacgcggc gcgaaaaccc
    20341 gcgacaccag caagtggacc gagtatctga acgaaacgct ggatatgtgg ggtaacgacg
    20401 cggaagtcct gtttatgccg cacacctggc cggtctgggg caataagcat atcaatgatt
    20461 atattggtaa atatcgcgat actatcaagt acattcacga ccagaccctg cacctggcga
    20521 accagggcta caccatgaat gaaatcggcg acatgattaa gctgccgcct gcacttgcca
    20581 ataactgggc cagccgtggc tattacggtt ctgtcagcca caacgcccgc gcggtgtata
    20641 acttctatct tggctattac gacggtaacc cggctaacct gcatccgtat ggtcaggtgg
    20701 agatgggtaa acgttacgtg caggcgctgg gcggttctgc ccgtgtcatc aacctggcgc
    20761 aagaagcgaa caagcaaggt gattaccgct ggtcggcaga actgctgaaa caggtgattg
    20821 ccgccaaccc gggtgaccag gtcgcgaaga atctgcaagc gaataacttt gaacagctgg
    20881 gctatcaggc cgagtccgcc acctggcgcg gtttctacct gaccggcgcg aaagagctgc
    20941 gcgaaggggt gcataagttc agccacggca ccaccggttc cccggacacc attcgcggga
    21001 tgtcggtcga aatgctgttc gactttatgt ccgttcgcct cgatagcgcg aaagccgcgg
    21061 gtaaaaatat cagcctgaac ttcaatatgg gcaacggcga taacctcaac ctgacgctga
    21121 acgatagtgt gcttaactac cggaaaacgc tgcaaccgca agccaacgcc tctttctaca
    21181 tcagccgtga agatctgcac gccgtgctga ccggacaggc aaaaatggcg gatctggtaa
    21241 aagcgaagaa agccaaaatt attggcaatg gcgcgaaact ggaagaaatt attgcctgtc
    21301 tggataattt cgatttgtgg gtgaatatcg taaccccaaa ttaatgccac tcgcttttgg
    21361 gggcgctgaa gcccccaaaa gcatttattt taaaaatata tacaaccaat taatacataa
    21421 aatatataag ttatgaatgc ggtggctgaa taaagctatt tttattcatc aagaaaatag
    21481 ccatactgac gccttcaatg gatattgata ttcactacag atattgttcg cagcagacag
    21541 gatgtattta atatgccaac agttctctct cgcatggcga tgcaactcaa aaaaacagcc
    21601 tggataattc ccgtcttcat ggtttcggga tgctcattat ctccggcaat cccggtgatc
    21661 ggcgcttatt atcccagctg gtttttctgc accattgcca gccttatttt gacgctcatc
    21721 acgaggcgag ttattcagcg ggcaaatatc aatctggcat ttgtcggaat tatttatacc
    21781 gccctttttg ctctctacgc catgctgttc tggctggcat ttttctaatc tatttgagac
    21841 gcgctcatgg aaagtacgcc gaaaaaagct cctcgcagta aattccctgc tctgttagtg
    21901 gttgcgttgg cgctggttgc ccttgttttc gttatctggc acgtagacag tgcgccatca
    21961 actaatgacg cttacgcgtc agcagatacc attgatgtgg taccggaagt cagcggccgc
    22021 attgtagaac tggcggtcac cgacaaccag gcagtcaaac agggcgattt gctgttccgc
    22081 atcgacccgc gcccgtacga agccaatctg gcgaaagctg aagcctccct cgcggcgctg
    22141 gataagcaaa ttatgctcac ccagcgtagc gttgacgcgc aacagtttgg tgccgactcg
    22201 gttaatgcca cggtagaaaa agcccgtgcc gccgcgaaac aggccacaga tacattacgc
    22261 cgcaccgagc cattactgaa agaaggtttt gtctcagcgg aagatgttga ccgtgcaaga
    22321 acggcgcagc gcgccgcaga agcggatctt aatgccgtat tgttacaggc gcagtcagcc
    22381 gccagcgccg tcagcggcgt ggatgcatta gttgcccagc gtgcggcggt cgaagcggat
    22441 attgccctga ccaaactgca tctggaaatg gcgaccgttc gcgcgccgtt tgatggccgg
    22501 gtcatttccc tcaaaacctc cgtcgggcaa tttgcttctg ccatgcgccc tatttttacc
    22561 ctaatcgaca ctcgtcactg gtatgtgatc gccaacttcc gcgaaaccga tctgaaaaat
    22621 attcgctcag gtacacccgc aacgattcgc ctgatgagtg acagcggcaa aaccttcgag
    22681 ggtaaagtgg attcgattgg ctacggcgtg ctaccggatg acggcggcct ggtgctgggc
    22741 ggcctgccga aagtgtctcg ttctattaac tgggtccgcg ttgcccagcg ttttccggtc
    22801 aaaatcatgg tcgataaacc tgacccggaa atgttccgca tcggcgcttc ggcagtcgct
    22861 aatcttgagc cgcaataatg agcgcgctca actccctgcc attaccggtg gtcaggctgc
    22921 tggcgttctt tcatgaagag ttaagcgagc ggcgaccagg tcgcgtgccg cagaccgtgc
    22981 aactctgggt aggctgcctg ctggtgattc tgatctcgat gacctttgag atcccttttg
    23041 tggcgttatc gctggcagtg ctgttttacg gtattcagtc gaacgcgttt tacaccaaat
    23101 ttgtcgcgat cttgtttgtg gttgccacgg tgctggagat cggcagcctg tttttgatct
    23161 acaaatggtc atacggcgaa ccgttgatcc gattgatcat cgccggaccg atcctgatgg
    23221 gctgcatgtt tttgatgcgc acccatcgct tggggctggt ctttttcgcc gtcgccattg
    23281 tcgctattta cgggcaaacc ttccccgcca tgctcgacta tccggaagtg gtcgtgcgct
    23341 taacgctgtg gt
//
LOCUS       DACAPB010000056        23134 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.056, whole genome shotgun sequence.
ACCESSION   DACAPB010000056 DACAPB010000000
VERSION     DACAPB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.056"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(164..802)
                     /gene="leuE"
                     /locus_tag="IT904_002002"
     CDS             complement(164..802)
                     /gene="leuE"
                     /locus_tag="IT904_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAP3191786.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILAATL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(929..1852)
                     /locus_tag="IT904_002003"
     CDS             complement(929..1852)
                     /locus_tag="IT904_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191787.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1955..3040
                     /gene="dmlA"
                     /locus_tag="IT904_002004"
     CDS             1955..3040
                     /gene="dmlA"
                     /locus_tag="IT904_002004"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HAP3191788.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3291..4901
                     /gene="yeaV"
                     /locus_tag="IT904_002005"
     CDS             3291..4901
                     /gene="yeaV"
                     /locus_tag="IT904_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HAP3191789.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            4933..6057
                     /gene="yeaW"
                     /locus_tag="IT904_002006"
     CDS             4933..6057
                     /gene="yeaW"
                     /locus_tag="IT904_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HAP3191790.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6113..7078
                     /gene="yeaX"
                     /locus_tag="IT904_002007"
     CDS             6113..7078
                     /gene="yeaX"
                     /locus_tag="IT904_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAP3191791.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLVCCSRAKGKRLVLDL"
     gene            complement(7132..8247)
                     /gene="rnd"
                     /locus_tag="IT904_002008"
     CDS             complement(7132..8247)
                     /gene="rnd"
                     /locus_tag="IT904_002008"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAP3191792.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDMTNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8329..10014)
                     /gene="fadD"
                     /locus_tag="IT904_002009"
     CDS             complement(8329..10014)
                     /gene="fadD"
                     /locus_tag="IT904_002009"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAP3191793.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10219..10800)
                     /gene="yeaY"
                     /locus_tag="IT904_002010"
     CDS             complement(10219..10800)
                     /gene="yeaY"
                     /locus_tag="IT904_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAP3191794.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10840..11535)
                     /gene="tsaB"
                     /locus_tag="IT904_002011"
     CDS             complement(10840..11535)
                     /gene="tsaB"
                     /locus_tag="IT904_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP3191795.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(11593..13503)
                     /locus_tag="IT904_002012"
     CDS             complement(11593..13503)
                     /locus_tag="IT904_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP3191796.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVVVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13635..13979
                     /locus_tag="IT904_002013"
     CDS             13635..13979
                     /locus_tag="IT904_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP3191797.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            14341..14700
                     /locus_tag="IT904_002014"
     CDS             14341..14700
                     /locus_tag="IT904_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAP3191798.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(14820..14999)
                     /locus_tag="IT904_002015"
     CDS             complement(14820..14999)
                     /locus_tag="IT904_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAP3191799.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            15073..16434
                     /gene="pabB"
                     /locus_tag="IT904_002016"
     CDS             15073..16434
                     /gene="pabB"
                     /locus_tag="IT904_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAP3191800.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQGYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            16438..17016
                     /locus_tag="IT904_002017"
     CDS             16438..17016
                     /locus_tag="IT904_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAP3191801.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            17200..18564
                     /locus_tag="IT904_002018"
     CDS             17200..18564
                     /locus_tag="IT904_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HAP3191802.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            18695..20293
                     /locus_tag="IT904_002019"
     CDS             18695..20293
                     /locus_tag="IT904_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP3191803.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(20297..21853)
                     /gene="yoaE"
                     /locus_tag="IT904_002020"
     CDS             complement(20297..21853)
                     /gene="yoaE"
                     /locus_tag="IT904_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAP3191804.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            22316..>23134
                     /gene="manX"
                     /locus_tag="IT904_002021"
     CDS             22316..>23134
                     /gene="manX"
                     /locus_tag="IT904_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP3191805.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQG"
BASE COUNT         5694 a         6033 c         5755 g         5652 t
ORIGIN      
        1 tccttcgggg aatgcgctga aaacggttta ttccagccgt ttcagggtac gcctgataat
       61 ttgcatttta aataccattt attggttact ttttagcacc atatcagcga agaatcaggg
      121 aggattatag atgggaagcc cgcgacaacg cgggctgaaa gcatcaggat tgcagcgtcg
      181 ccagtcgggc agcgaaaccc acgaacatca aaccaatcag tgagttgcca actttagcca
      241 gtttcttttt ggtacgtatg tactgcgtga caaaagcacc agatataatc aggaagctca
      301 aatagcagaa actcaccagt tccagcgtcg ccgccagaat aaagaatgaa attcccgtat
      361 gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa atggctttcg
      421 gattagtcag gctcaaaatt aacgcgcgtt taaaaatagc accgtattgg ggctcatcgg
      481 atttggcctc gctattttta cccttcaggg tcgcgtaaag aattttactc cccagataga
      541 gcaaataaaa cgcaccaaga taacgtacaa tgttgaataa tatcggggtg gtcttaatta
      601 atgtcgccac tccagcccat gccagaaaca tcaataccgc atcgccaata aatacaccgc
      661 aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt acaaacaggg
      721 tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc cagtaattca
      781 gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca tgcgcgatta
      841 aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag gttttaacct
      901 gatatcaacc cgataattga atcattaatc aggcatgctc cagtgaaaaa ttcgggtagt
      961 gctcggcaaa atactggcgt aaaaattcta ccgttatccg cactttcgct gacgtcgcca
     1021 gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt agcacttgca
     1081 ctaaatgacc actggcaatg ttttcgctaa catcccacca ggaacgcaga gcaatcccct
     1141 gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt gggccggtga
     1201 ctttaatcgc atgggggcct tctttattgc gtaattgcca gacaccaaaa ggatgatcgc
     1261 gctctttaat gaccaggcac ggcaatgcag aaagatcggt taaatgtttc ggcgcgccat
     1321 gttgcgcaat aaattccggc gacgcgcaga gaatacggta attggtcgcc agtttgcggg
     1381 cgattaaatt aggggcgatg tcatcgccaa tgcgaatatc gagatcgaca ccttcattga
     1441 ccaaatcgac cagtcggtct tccacatcaa aacgtaattc cagttgcgga tacgccttcg
     1501 ccagcgctga taacgccgga gccaccacct gtcgtccaaa accaaagctg ctgataatac
     1561 gcagcatccc ctgcggcacc tgacgcacgt cagaaagttc gtccatcatc tgaccgacat
     1621 cctgcaaaat ccgctgcgcc cattcataaa tccgctctcc ttcttcggta atggtgacgc
     1681 ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag gcgatgcgct
     1741 tgctgacgaa cgccggtgaa acgcccagtt cttccgccac ggcggcaaaa ccagcccggc
     1801 gagccaccag cataaagacg cgcaaatcat tcagcagcgg taaattattc atgattcgtg
     1861 ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag gatggtgatg
     1921 ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc gtattgctgc
     1981 gatcccggga gacgggattg gcaaagaagt ccttcctgaa gggattcgcg tgttacaggc
     2041 tgccgctgag cgctggggct tcgccttgag ttttgagcaa atggagtggg cgagctgcga
     2101 gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac ttagccgttt
     2161 cgacgccatc tattttggtg ccgtcggctg gccggatacc gttccggacc atatttcgtt
     2221 gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc tgcgcccggt
     2281 tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg acatcgattt
     2341 ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggta gagtgaatga
     2401 aggtacagag catgaagtcg tcattcagga atcggtattt acgcgtcgtg gtgtcgatcg
     2461 cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc taacttctgc
     2521 cactaaatcg aacggtttag ccatcagcat gccgtactgg gatgagcgag tggaagcaat
     2581 ggccgagaat tacccggaga tccgctggga caagcagcat attgatattc tctgcgcgcg
     2641 ttttgtgatg cagccggaac gcttcgatgt ggtggtggcg tccaatttgt ttggcgatat
     2701 cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat ccgccaacct
     2761 gaatccggaa cgcactttcc cgtcgctctt cgagcctgtc cacggttccg cgccggatat
     2821 ctacgggaaa aatattgcta accctatcgc cacgatttgg gccggggcaa tgatgctcga
     2881 ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc tggcagcaat
     2941 tgaagaagtg attgctcacg ggccgaaaac acctgatatg aaaggcaatg ccaccacgcc
     3001 acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca gtttatttga
     3061 accgcgtcac tgacgcggtt tttttattcg ttctttgcag taaataacct gcgtcatttc
     3121 accttttatt gtttccgttt cgtgttttat ggctttccgt attcttaatt gcttaattta
     3181 tgtaacatac aaataatcaa tttgttaaac acaacataca actcattacc ctgcaaataa
     3241 ttacaactgg caaaagccag agacgggctt ttatgcccag gagatgactc atgatgagca
     3301 atgttaagaa aaaagatgtg ccgctgataa gcatcagcct ggtggccatt cttttcatcg
     3361 cagctgcatt aagccttttc ccacaacaat cggccgacgc ggccaacgca atatacactt
     3421 ttgttactcg tacgttaggt tccgccgtac aggtattggt tttgctggca atgggactgg
     3481 tgatttattt agccaccagt aaatacggca atattcgtct tggcgaagga aaaccggaat
     3541 acagcacgct ctcctggctg tttatgttta tttgtgccgg tttaggttct tctacgcttt
     3601 attggggggt tgctgaatgg gcctattatt atcaaacacc tggattaaat atcgcaccgc
     3661 gttcacaaca ggcactcgaa tttagcgttc cctactcttt cttccactgg ggcatcagcg
     3721 cctgggcaac ttatacgctg gcctcattaa tcatggctta tcactttcat gtgcggaaaa
     3781 acaaaggtct gagcctttcc ggcattattg ctgctattac cggcgttcgc ccgcaaggcc
     3841 catggggaaa actggtcgat ttgatgttcc tgatcgccac tgtcggcgca ctgaccattt
     3901 cccttgttgt taccgcagca acctttaccc gtgggctttc cgcgctgacc ggtttacccg
     3961 ataacttcac cgtgcaggca tttgtgatcc tgctttccgg cggcattttt tgcctaagct
     4021 cgtggattgg tatcaacaac ggtttgcaac gtctgagcaa aatggttggc tggggcgcgt
     4081 tcctgctgcc attactggtg ctgattgtcg gcccaaccga atttattacc aacagcatca
     4141 tcaatgccat cggcctgacc acgcaaaact tcctgcaaat gagcttattc accgatccgc
     4201 ttggcgatgg ttcatttacc cgcaactgga ccgttttcta ctggctgtgg tggatctcat
     4261 acacccctgg cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag attaaagaag
     4321 ttatctgggg actgatcctc ggcagcaccg tcggttgctg gttcttcttt ggcgtaatgg
     4381 aaagctatgc cattcatcag tttatcaatg gcgtaatcaa cgtcccacag gtgctggaaa
     4441 cactgggcgg cgaaacagct gtacagcaag ttctgatgtc gttgccagcc ggtaaattgt
     4501 tcctcgccgc atacctgggc gtgatgatta ttttccttgc ctcgcatatg gatgcagtgg
     4561 cctacaccat ggcggcgacc agtacgcgta atctccagga aggtgacgat cctgaccgtg
     4621 ggctgcgtct tttctggtgc gtggtgatca ctctgatccc gctttccatc ttgtttaccg
     4681 gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc ttcctcgtca
     4741 ttttactggt gaaagtcggc gggtttattc gctggctgaa acaggattac gccgacattc
     4801 cggctcatca agttgaacat tatctcccgc agacaccggt tgaagccctg gaaaaaacgc
     4861 cagtgctccc tgcgggaacc gtattcaaag gcgacaactg agcgccatca tcctaacgat
     4921 aaaggtatcc ctatgagcaa tctgagccct gactttgtac tacccgaaaa tttttgcgct
     4981 aacccgcaag aggcgtggac cattcctgcc cgtttttata ccgatcagaa cgcgtttgaa
     5041 cacgaaaaag agaacgtctt cgccaaaagt tggatttgcg tcgctcacag cagcgaactg
     5101 gcgaatgcca atgattatgt gacgcgtgag atcattggcg aaagcatcgt gctggtacgc
     5161 ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg tcatcagttg
     5221 ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc cgtatcacgc atgggcattc
     5281 aaactcgacg gcaacctggc ccatgcacgt aactgcgaaa acgtcgccaa tttcgatagc
     5341 gacaaagcgc aactggttcc ggtgcgtctg gaagaatatg ccggattcgt cttcatcaac
     5401 atggacccca acgccaccag cgtagaagat caattacccg gcctgggcgc gaaagtgctg
     5461 gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc gctttaccac ccgcacgcct
     5521 gccaactgga agaacattgt cgataactat ctcgagtgct atcactgtgg tccggcgcat
     5581 ccaggtttct ccgactccgt acaggttgat cgttactggc acaccatgca cggtaactgg
     5641 acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga agagggtacg
     5701 gatgcggcat tccacggttt ctggctgtgg ccgtgcacga tgctgaacgt caccccgatc
     5761 aaagggatga tgacggtcat ttatgaattc ccggtggatt ctgaaactac cctgcaaaac
     5821 tacgatattt acttcaccaa tgaagagtta accgacgagc aaaaatcgct gattgagtgg
     5881 tatcgcgatg tgttccgtcc ggaagattta cgtctggttg aaagcgtaca gaaagggctg
     5941 aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg acagtagcgg tagtggcatt
     6001 tccgaacatg gtatcgccca tttccataat ctgctggcgc aggtgtttaa ggactaatga
     6061 catcggcggc ggtattttcc gccgctgggc tgatttttga tggagtacag caatgtcaga
     6121 ctatcaaatg tttgaagtac aggtgagcca ggttgaaccc cttaccgaac aggtgaaacg
     6181 cttcacgctg gtggcaaccg atggcaaacc attacctgcg tttaccggag gaagtcacgt
     6241 cattgtgcag atgagcgatg gtgataacca gtacagcaat gcgtattcac tactgagttc
     6301 gccgcatgac acctcttgtt atcagattgc cgttcggctg gaggaaaact cgcgcggcgg
     6361 ttcccgcttt ttgcatcagc aggtaaaagt gggcaatcgg ttaacgattt caacgcctaa
     6421 taacctgttt gcgctaattc cctcagccag aaagcatctg tttatcgcgg gcggtattgg
     6481 tatcacccct ttcctgtcgc acatggcaga gctgcaacac agcgacgtcg actggcagct
     6541 acattactgc tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag tccagcatcc
     6601 gcaggctgag aaagttcact tgcatcactc atcaaccgga acacgactgg aattagcgcg
     6661 attattggcg gatatcgaac ctggcacaca cgtttatacc tgtggtcccg aggcgctaaa
     6721 tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc gccgatacgc tgcactttga
     6781 gcaatttgct atcgaagaca aaaccggcga tgcctttacc ctggtgcttg cccgttccgg
     6841 aaaagagttt gtggtgccgg aagagatgac tattttgcag gttattgaaa ataataaagc
     6901 cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa cagcaatact
     6961 ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg ccagccagca
     7021 aagtatgttg gtctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg atttgtagtt
     7081 tgccaaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat tttactgcgg
     7141 atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc agccggaaat
     7201 cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca gttggttgat
     7261 ttgccgacgc gatgccagca attcggcgct gatcttatgc gtttcgctca cgtcagtaat
     7321 cagcgactta atcgctttaa acgctttacg ataacccggc atgtccatca ggttaagcat
     7381 cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg ccagcagcgt
     7441 tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt cgcctaaact
     7501 tcccggcata taacgcgcga ccgaccacaa atgctcttca cgcacgacaa agttcaccgc
     7561 cagatcacgc tcacgagcct tgcgcagtcg ccagtcggct aacagttgca gacaggccag
     7621 ttggcgtgtg cgtaattgcc aggcattggt catatcacgc caggcatctt ccggcgcaac
     7681 gacttcctga cgacgcattt gcatcaggcg gcattcatcc agcgccgcag gtagccagcc
     7741 ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc agacatcagc
     7801 cgctgcgtat tcacactgac gttcggtcag cggtctggcc agccagtcgg tgcgcgattc
     7861 actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaacc cccatgacat
     7921 cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc aggggttgtg gtaattcgcc
     7981 aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt ttgtgatgga
     8041 cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag gatcaattaa
     8101 cgccagatgc tcaccatcaa aaagttgaat taaacccagc tgcgggtaat aagtgcgcgt
     8161 acgaacaaat tcagtatcca gggctatcgc cggaaaggca cggacggctt cacacaaaga
     8221 agccagcgca tcgtccgtgg taatcatttg gtaattcaaa ttgtattctc tttagtgggc
     8281 gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc aggctttatt
     8341 gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc cgacgttaga
     8401 tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg tgagctgacg
     8461 gcggcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca ctacgaagat
     8521 tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt cctgtacgcc
     8581 aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac cggaaaccag
     8641 aatcatgtct tttttacgat cgacaatgcg caggaaccct tcttcatcca tcaccgcgat
     8701 gtcgccggtg tgtaaccagc catttttgat gatttcatcg gtagcatcgg gacgctgcca
     8761 gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac ctggtggtac
     8821 ttcattatca tcatcatcca ccagtttggc ttccgtcgac ggcaccggca aaccgatgct
     8881 accactatga taatcaatat catatgggtt aacgctgacc agcggcgcac actcggtaag
     8941 gccatagcct tccagcagat actgtccggt cagtttcacc caacgctctg ccaccacttg
     9001 ctgcactggc atcccaccgc ctgcggaaag atgcagactg gagaaatcca gctgctggaa
     9061 ctctttattg ttcagcaacg cattgaacaa ggtgttaacg ccagtgatag cggtaaacgg
     9121 atatttcgct aactctttta ccaaccctgg aatatcacgc gggttagtga taagcaggtt
     9181 ctgcccaccc agttcgataa acagcaggca gttaatggtc agggcaaaaa tgtgatacag
     9241 cggcagtgcc gttactacca gttctttacc tggatgcagc aacggcccat aggtggcgtt
     9301 gacctgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt tcgccacacc
     9361 tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc ggcaccagtt cgggtttgac
     9421 gtactgcatc cggtagccgt tatgcagtgc gctacgaaat gaaatggcat ctggcagatg
     9481 gtatttcggc accaaacgct tgatgtattt aacaacgaaa ttgactaccg tgccttttgc
     9541 cgtagatagc tgatcgccca tacgggtcag aattacgtgc tgaacggcgg ttttatcaac
     9601 cactttttcc agtgtgtgag caaagttaga cacgataaca atcgccgatg cgccgctatc
     9661 gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta cgacgatcat
     9721 cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag gcatcatcaa
     9781 cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg caaacgcgcg
     9841 actgcgttct tccagcttgc ggaaggtcat cacctccccc atattcacaa acgcaggctg
     9901 atcggcgtag cgcgcgaccg actgctcaaa catatctacc agagattgat aacggtcagg
     9961 gttgatctcc gtcggaacgt ccgcgggata acggttaagc caaaccttct tcaattcttc
    10021 acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat gatatacatg ctgttaacat
    10081 aatattaact catcatacca gcttgataat tacccaacga aaaggttgcg aagcgcgtca
    10141 ctatttattt ttatctttac cgtaagaatg cagaaacagc ggaccagccg ctgtttcttt
    10201 ttctttaaaa acaagcaatt attcagttac aactgtttgt actctcgcgg gaccgggatt
    10261 ataccagccc catccgccgt agccataggg ccagccacga ccgccataaa accatggatc
    10321 aatcggctga ggcggcataa tcacctgctg ggttaaatgc caacgtttgt aacccgttac
    10381 ttgcatcacc ataaatttat agggcgtatt gccgattttg ccgtcaaccg caccagtgat
    10441 tggcccgact accgtaacca gttgtccacg gaaatccacc gggtccagaa aaccgttcac
    10501 atcggcataa atgcgaccgc gagaaggttc tcccagcgtc ggtctggctc cgctgtccag
    10561 cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtaccg caaccacttt
    10621 gccaccaaag cgtgcctcct gaccaacgta cagctgcggc gcactcatca cccgaactaa
    10681 atcttgttgc ggcgtgggac tgctgccttt aatggcgtcc ggcacagtga cacaaccgct
    10741 cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt gaaccgccat
    10801 ggtgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc ccggaagttt
    10861 cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg ccaccgtttt
    10921 accctcggca aacatctgac acgcaatcgg cagcatatct tcagcagcag gcagtaacac
    10981 ttcgccatcg cgcaaaacca gcccgctctc tttaccgaga tccggccaag cttgccagcc
    11041 cgtccctacc gtcacccatt cgccggaaag ctgttgcatt cgttcatgga cgatttcggg
    11101 tttgagtacg gcttcggttt cttcaccgtg ccagataccg ttttcatcac gctgatattc
    11161 ggcccagtaa acttcgccca ttcgcgcgtc aatggctgcc agcacgcggg ttgcgccgtt
    11221 tttgcgccac gccccttgcg ccatcgtcat tagcgtggag acgccaatca tcggtaattc
    11281 cgcgccaagc gccagccctt gcgcgatgcc aataccaatg cgcacgccag taaagctacc
    11341 ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg gaagttccgc tggtggtcag
    11401 gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa gctcaaaatg
    11461 agcgttgaca gtaccgtcgt tccacagggc gacagagcag gcctctgtcg cggtatcgat
    11521 agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca tcataccata
    11581 ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac gggcaatgtc
    11641 acgggtgcgt ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag gacgcatcac
    11701 cagccgattg tcacaaatca ccagcacgcc acgatcgtcg gcgtcgcgaa tcagtcgccc
    11761 taccccctgt ttgagagtaa tgacggcatc tggtagttgc acttcatcga acgggtcgcc
    11821 accgcgcaaa cgacaatctt ccatgcgcgc ttttaacagt ggatcatccg gcgaggtaaa
    11881 cggcaatttg tcgataatta ccaatgacaa tgtatcgcca cgcacgtcca ccccttccca
    11941 gaaactactg gttgccacaa gaagcgcatt accggcactg acaaactgct gcaacagttg
    12001 gcctttactg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc ggaactgctc
    12061 ggccagatcg cgcatcatgg cgtgcgaggt acaaagcata aaacaacgac cgttgttagc
    12121 ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca gaacctggct ggttggtttg
    12181 cggcagattg cgcggcacac agagtaacgc ctggcggctg taatcaaacg ggcttggcaa
    12241 tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca gatcgtcgtt
    12301 caccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca ttaactcttt
    12361 gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg aagtgcattc
    12421 gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc gtgtgcgata
    12481 caacgtggcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg acagtttcgc
    12541 cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct gaatttgcgg
    12601 attagctaac agctcacgca ggttaccgcg ataacctggc tcaccgagtt gcagacgaaa
    12661 atcctgcgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg tgtcttttaa
    12721 ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc gactggagag
    12781 tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt cgaagatcat
    12841 gacgtccgct tccgggatca gttcgccaaa tccactctct ttaaccacca tatccgccag
    12901 aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac gtgctttgac
    12961 cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt tggtgctggt
    13021 gaccagcggc cacgcctgtg aatcttccgc cacgctgacg caggtgctga tatcaccatc
    13081 gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaagattt gtaccggcag
    13141 atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt ttgagcgccc
    13201 tttcagcagc gccacgttgc ccgtatattt caatgccttt gagactgttg gcaaatcgcg
    13261 gctgtagagc tgatcctgca acgcttttga gccggtcgag ataatgactt tctttttcgc
    13321 ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta ccggttcctg cttccaccac
    13381 cagcggctgg cctttttcta tcgcctgggt gacgactacc gccatctgtc gctgtggttc
    13441 tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa aatcgtccgt
    13501 cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc tttcgccagt
    13561 tgaagtggtg acggcgacct cacattgtgg cagtctttgc agcacgaaaa tggaagtata
    13621 atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg tctgatgtag
    13681 taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac gccgatgcct
    13741 ttgagcaaac cgccaacacg ctggcacaga ttgacgccgt gctggaaaaa cagggcagca
    13801 ataaatcgag cattctggat gccaccattt tcctggccga taaaaacgac ttcgcggcga
    13861 tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc gccggtgcgc tgcacggtgc
    13921 aagcggggct gatgaacccg aagtataaag ttgaaattaa gattgtggct gcggtgtaag
    13981 ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc ggttttacat
    14041 aaatcgacca gagattaaga tattactgtt gatgctcctg ctaattcatt atttattttt
    14101 ataatttcaa ttttagctat aaagctaact atataaataa acataaccat gccgttgtgt
    14161 tcgcccttcg attagcacct ctggttttct ttcttagctg aacaatctgg ttatagcgtg
    14221 cggactgacc gttaattttc tccatcttac tggataacac accatgagct gctaccttta
    14281 aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag
    14341 atgcaaatta aggtcatata tagccttatc gataacatgg ttaattttaa ggataagaat
    14401 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
    14461 acaccaagtt cgatgaatga aagcacagcg aagggaatct ttaaatattt aaaagaactg
    14521 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
    14581 gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agtctggtga acgcactgta
    14641 attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga
    14701 gtttaattta tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
    14761 tttttctcac aatcaaataa tcatcggatt ctataacgaa atcgatacgg attagtgtgt
    14821 tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc
    14881 tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg
    14941 atccgctcga cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt
    15001 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
    15061 gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac
    15121 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
    15181 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact
    15241 ttaaccactt tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
    15301 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat
    15361 aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc
    15421 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
    15481 ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt
    15541 cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa
    15601 gatttcacgc tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa
    15661 tttcgccagg tacagggata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
    15721 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
    15781 aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg
    15841 ccagagcggt ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg
    15901 ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca
    15961 gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
    16021 gttgccgtag ccggttcggt aaaagtacca gagctgttcg tggtggaacc cttccctgcc
    16081 gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat
    16141 ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
    16201 gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat
    16261 ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac
    16321 ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa
    16381 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
    16441 gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa
    16501 attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga
    16561 ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa
    16621 gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg
    16681 cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg
    16741 ccgcccgtcg atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc
    16801 gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc
    16861 caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat
    16921 cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
    16981 cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa
    17041 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
    17101 tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt
    17161 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
    17221 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
    17281 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
    17341 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
    17401 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
    17461 cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
    17521 ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg
    17581 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
    17641 ggtggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg
    17701 ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg
    17761 ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
    17821 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa
    17881 ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgtta
    17941 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
    18001 gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac
    18061 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc
    18121 agcccggaca tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa
    18181 atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt
    18241 tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg
    18301 gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag
    18361 gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca
    18421 cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
    18481 atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg
    18541 gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa
    18601 tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag
    18661 gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa
    18721 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc
    18781 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
    18841 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga
    18901 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
    18961 ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg
    19021 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
    19081 accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt
    19141 gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat
    19201 cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct
    19261 gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc
    19321 tgaagacaat gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa
    19381 tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
    19441 gctgctaagt ttactggtgg gctacatcgc ctggctggcg accgcttacc ggatgagctt
    19501 ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt
    19561 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc
    19621 gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
    19681 tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat
    19741 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
    19801 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
    19861 aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg
    19921 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct
    19981 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
    20041 tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat
    20101 tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg
    20161 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
    20221 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat
    20281 gccgttacgt taatgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
    20341 ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa
    20401 tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg
    20461 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
    20521 ctgtaccgcc ttttaccagc cagccgtcac catcagtaat gatttccggc gtttcgtcag
    20581 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta
    20641 ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca
    20701 acaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg
    20761 ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt acaataccga
    20821 tgatttcatc cagttcaccg cgacataccg ggaacagact gtgcggtgaa gagagcagtt
    20881 gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg
    20941 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt
    21001 cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct
    21061 gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc
    21121 gcagcggcaa agtcgactgg tggcgaataa agttgcgacg cgcaatctgg ttaaacactt
    21181 cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga
    21241 aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca
    21301 ccgtggggtg ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca
    21361 tcgcaatcac taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa
    21421 ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga
    21481 aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca
    21541 gttcggttgt tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc
    21601 cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa
    21661 tcagcgacag cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt
    21721 tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga
    21781 tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca
    21841 ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc
    21901 aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gtgccagaaa ggcaggcgtt
    21961 aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata
    22021 ctccatggtt gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt
    22081 taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc
    22141 gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc
    22201 gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg
    22261 attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac
    22321 cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga
    22381 aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc
    22441 cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt
    22501 gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt
    22561 cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt
    22621 aatggctcgt gatgatgacc caagctttga tgaactggtt gcgctggcag tagaaacagg
    22681 ccgtgaaggc gtgaaagcac tgaaagccaa accggttgaa aaagccgcgc cagcacccgc
    22741 tgccgcagca ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat
    22801 ggttattggc cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg
    22861 gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac
    22921 cgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga
    22981 tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac gcgtaatgct
    23041 gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc
    23101 tgttaacgtc ggtggtatgg cattccgtca gggt
//
LOCUS       DACAPB010000057        23041 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.057, whole genome shotgun sequence.
ACCESSION   DACAPB010000057 DACAPB010000000
VERSION     DACAPB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.057"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(158..1000)
                     /gene="purU"
                     /locus_tag="IT904_002022"
     CDS             complement(158..1000)
                     /gene="purU"
                     /locus_tag="IT904_002022"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAP3191806.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1050..1508)
                     /locus_tag="IT904_002023"
     CDS             complement(1050..1508)
                     /locus_tag="IT904_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAP3191807.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1621..2526
                     /gene="rssA"
                     /locus_tag="IT904_002024"
     CDS             1621..2526
                     /gene="rssA"
                     /locus_tag="IT904_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAP3191808.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2618..3631
                     /gene="rssB"
                     /locus_tag="IT904_002025"
     CDS             2618..3631
                     /gene="rssB"
                     /locus_tag="IT904_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAP3191809.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3833..4741
                     /gene="galU"
                     /locus_tag="IT904_002026"
     CDS             3833..4741
                     /gene="galU"
                     /locus_tag="IT904_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP3191810.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4885..5298)
                     /gene="hns"
                     /locus_tag="IT904_002027"
     CDS             complement(4885..5298)
                     /gene="hns"
                     /locus_tag="IT904_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAP3191811.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5902..6519
                     /gene="tdk"
                     /locus_tag="IT904_002028"
     CDS             5902..6519
                     /gene="tdk"
                     /locus_tag="IT904_002028"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP3191812.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRSEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(6800..7696)
                     /locus_tag="IT904_002029"
                     /pseudo
     CDS             complement(6800..7696)
                     /locus_tag="IT904_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(7820..10495)
                     /gene="adhE"
                     /locus_tag="IT904_002030"
                     /gene_synonym="adhC"
     CDS             complement(7820..10495)
                     /gene="adhE"
                     /locus_tag="IT904_002030"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP3191813.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            10972..11619
                     /gene="ychE"
                     /locus_tag="IT904_002031"
     CDS             10972..11619
                     /gene="ychE"
                     /locus_tag="IT904_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAP3191814.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(11777..12073)
                     /locus_tag="IT904_002032"
     CDS             complement(11777..12073)
                     /locus_tag="IT904_002032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191815.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            12357..13988
                     /gene="oppA"
                     /locus_tag="IT904_002033"
     CDS             12357..13988
                     /gene="oppA"
                     /locus_tag="IT904_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAP3191816.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKTWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            14074..14994
                     /gene="oppB"
                     /locus_tag="IT904_002034"
     CDS             14074..14994
                     /gene="oppB"
                     /locus_tag="IT904_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAP3191817.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            15009..15917
                     /gene="oppC"
                     /locus_tag="IT904_002035"
     CDS             15009..15917
                     /gene="oppC"
                     /locus_tag="IT904_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAP3191818.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            15929..16942
                     /gene="oppD"
                     /locus_tag="IT904_002036"
     CDS             15929..16942
                     /gene="oppD"
                     /locus_tag="IT904_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAP3191819.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            16939..17943
                     /gene="oppF"
                     /locus_tag="IT904_002037"
     CDS             16939..17943
                     /gene="oppF"
                     /locus_tag="IT904_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAP3191820.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(17996..18325)
                     /locus_tag="IT904_002038"
     CDS             complement(17996..18325)
                     /locus_tag="IT904_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAP3191821.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(18360..19820)
                     /gene="cls"
                     /locus_tag="IT904_002039"
     CDS             complement(18360..19820)
                     /gene="cls"
                     /locus_tag="IT904_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP3191822.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            19963..20136
                     /locus_tag="IT904_002040"
     CDS             19963..20136
                     /locus_tag="IT904_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAP3191823.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(20191..21444)
                     /gene="kch"
                     /locus_tag="IT904_002041"
     CDS             complement(20191..21444)
                     /gene="kch"
                     /locus_tag="IT904_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAP3191824.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(21744..22040)
                     /locus_tag="IT904_002042"
     CDS             complement(21744..22040)
                     /locus_tag="IT904_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAP3191825.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            22392..>23041
                     /locus_tag="IT904_002043"
     CDS             22392..>23041
                     /locus_tag="IT904_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191826.1"
                     /translation="MSNTTDWGSMIANIVMATTAVWAAFKANDWFKTKRLESAENFYK
                     EVHNLHKRYYSAIQYVEDSRRIFDSQYGLHSPISEPKDKSLQWEVKSNSYKIIAELDY
                     LASSLQMMDAMGNKLSPKSASLVSDMMEAHGRFCNDVMNFNDSVWKVIRAGITEENIP
                     HTRGYVNGDYMAILTSMDEPVRISFKLKEISFKDFYLHETTMQSVITRLKSLIARTG"
BASE COUNT         6016 a         5240 c         5662 g         6123 t
ORIGIN      
        1 ggggtaatgc tttttactgg cctgctccct tatcgggaag cggggcgcat catatcaaat
       61 gacgcgccgc tgtaaagtgt tacgttgaga aaaatgaact ggttgcgtaa ttttcatccg
      121 taacggatta aaggtaacca gttatttttg ctggcgatta aagaataatc gtccgattac
      181 cgtaaacaaa gacgcgctgt gccagtactt tgtatagtgc acgacttaag acgtttttct
      241 cgacgtcacg acctgcgcgc atcatatctt cagctgtgta ggtatgatcg acatgaataa
      301 cgtcctgcat gatgattggg ccttcgtcca gattgtcatt cacatagtga gcggttgcgc
      361 caataatctt cacaccacgt tcataggcct ggtgataagg acgtgcgcca ataaacgctg
      421 gcaggaatga atggtgaata ttgatgatct tattcgggaa gcgtgccaca aattccggcg
      481 ttaatacccg catatacttc gccagcacca cgtagtcagg ttgataagca tcaatggcat
      541 ccgccatctt ttgatcgtgc tcgttgcggc ttaacccttc atggcttacc agctcaaacg
      601 gaatatcaaa acgctcaacc agagaacgta gagtatcgtg gttaccaata actgccgcga
      661 tttcgacatc caggccgccg taattggctt tcatcaacaa atcgccaagg caatgcgctt
      721 ctttagtgac cagaatcact atccggcgac gaccggcagg attcagctca cgcacggagc
      781 cttctggcaa tgcgctatcg agatccgcca gcagggtgga atcattaaaa atcccttcca
      841 gttccgtgcg cataaaaaag cgcccggtac ggtgatcaac aaattcattg ttctgtacga
      901 tatttaactc gtgcttgtag caaatattgg taatacgtgc gatcagacct ttttggtccg
      961 gacaaatagt acgcagaact ttacgttgga gtgaatgcat tgctggaaaa accttgttga
     1021 gagtgtttgc taaaccgtac cgtcaaccat tattggccgc agcacttttt aaatttttta
     1081 cctgaaccac aagggcaggg atcgttgcgg ccaaactgcg gacgtgtacc gtcaatatag
     1141 taccattgac cgttttcttt caaaaatcgc gaacgttcaa ttattgcacc tgttttgcca
     1201 ccttcggtaa atcgagcaac aaaactgaca aatccgatat tgtcggcgtc ctgccaacaa
     1261 tgttcaaata cggtcaatcc caaccattca gtatgcgcaa atccggcaat caattcggca
     1321 cgtaacgccg ctgctccaca agatggatgc caggtcttta ttaaataatc tgcgtcttgc
     1381 atcacaaaag cgcagtaacg cgaacgcatg agatgttctg gatcaggtgc aaccttttca
     1441 ccagacacat aagggtggca acataggcta tactcgacag cactaccaca gggacaaagc
     1501 tgagacacaa ataatctccc tggaaacaat aacggcgtat taaccgcctg agtagcacta
     1561 tgttaaccga gcagtagcga tgtggctacg attgcattcc aggggaatct tgcgggaata
     1621 atgagaaaga taaaaatagg gctggcgctg ggatctggcg cggcgagagg ttggtcgcat
     1681 attggcgtta ttaatgcgct aaaaaaagtg ggtattgaaa ttgatatcgt tgcaggatgt
     1741 tcaattggtt cgctggtggg cgctgcctat gcatgcgatc gattatctgc gctggaagat
     1801 tgggtgacct ctttcagtta ttgggatgtt ttacgcctga tggatctctc ctggcagcgc
     1861 ggtgggttac tgcgcggcga gcgtgtcttc aatcaatacc gcgaaataat gccggaaaca
     1921 gagatcgaaa attgttcccg tcgctttgcg gctgttgcca ccaatttaag tacgggacgt
     1981 gaattatggt ttactgaagg cgatctccat cttgctattc gcgcatcatg cagtattcca
     2041 ggactcatgg cacctgttgc acataacggc tactggctgg ttgatggagc agtcgttaac
     2101 ccaattccta tttccctcac gcgtgcattg ggggctgata ttgtgatagc ggttgacctg
     2161 cagcacgatg ctcatttgat gcaacaagat ttgctctcct ttaatgtcag tgaagaaaat
     2221 agcgagaatg gtgattctct gccgtggcat gcgcgtctga aagaaaggtt aggcagcata
     2281 acgacacgtc gggcggtgac agcgccaacg gcaacagaga ttatgaccac ttctatccag
     2341 gtgctggaga accgccttaa aaggaaccgc atggcaggtg atccgcccga tattctgatt
     2401 caacctgttt gcccgcaaat atctacgctt gatttccatc gcgcgcacgc tgccattgcg
     2461 gccggacagc tggcagtgga aaggaaaatg gacgaacttt tgccgttggt acgcaccaac
     2521 atttgaccag aatttttatc tacacttaag ttaattctga caggcgcagg tggcaatagc
     2581 atgccactat tgagtaaagc cagtcagggg agagaacatg acgcagccat tggtcggaaa
     2641 acagattctc attgttgaag atgagcaggt atttcgctcg cttctggatt catggttttc
     2701 ctcattggga gcgacaacgg tactggcggc tgatggggtg gatgcccttg agttgctggg
     2761 aggtttcact ccagacctga tgatatgtga tatcgcgatg ccacgaatga acgggcttaa
     2821 actgctggag catatacgta acagaggcga ccagacccca gttctggtga tatctgccac
     2881 tgaaaatatg gcagatattg ccaaagcgtt acgtctgggc gttgaagatg ttttgctgaa
     2941 accagttaaa gatctgaatc gcttgcgcga gatggttttt gcctgtctct atcccagcat
     3001 gtttaattcg cgcgttgagg aagaggaaag gctttttcgc gactgggatg caatggttga
     3061 taaccctgcc gcagcggcga aattattaca ggaactacaa ccgccggttc agcaggtgat
     3121 ttcccattgc cgggttaatt atcgtcaatt ggttgccgcg gacaaacccg gcctggtgct
     3181 tgatattgcc gcactttcgg aaaacgatct ggcattttat tgccttgatg tcacccgagc
     3241 tggacataat ggcgtacttg ctgccttgtt attacgcgca ttgtttaacg gattattaca
     3301 ggaacagctt gcacaccaaa atcaacggtt gccagagttg ggcgcgttat tgaagcaggt
     3361 aaaccattta cttcgtcagg ccaatctgcc ggggcagttt ccgctattag ttggctatta
     3421 tcatcgcgaa ctgaaaaatc tcattctggt ttctgcgggt ctgaatgcga cgttaaatac
     3481 cggcgaacac caggtgcaaa tcagtaatgg tgttccgtta ggcactttag gtaacgctta
     3541 tttgaatcaa ttgagccagc gatgcgatgc ctggcaatgc caaatatggg gaaccggtgg
     3601 tcgactgcgc ttgatgttgt ctgcagaatg agcaaacgat aacgcgggct aaatttgcat
     3661 tacctgctaa tgtcggctgg tggtactatc gtcgccattc gtataagtaa ttgtcttaat
     3721 tatgctaact cgcctccttt tcagaactta gccccttcgg ggtgctgata tactgggatg
     3781 cgatacagaa atatgaacac gttcaaaaca cgaacagtcc aggagaattt aaatggctgc
     3841 cattaatacg aaagtcaaaa aagccgttat ccccgttgcg ggattaggaa ccaggatgtt
     3901 gccggcgacg aaagccatcc cgaaagagat gctgccactt gtcgataagc cattaattca
     3961 atacgtcgtg aatgaatgta ttgcggctgg cattactgaa attgtgctgg ttacacactc
     4021 atctaaaaac tctattgaaa accactttga taccagtttt gaactggaag caatgctgga
     4081 aaaacgtgta aaacgtcaac tgcttgatga agtgcagtct atttgtccac cgcacgtgac
     4141 tattatgcaa gttcgtcagg gtctggcgaa aggcctggga cacgcggtat tgtgtgctca
     4201 cccggtagtg ggtgatgaac cggtagctgt tattttgcct gatgttattc tggatgaata
     4261 tgaatccgat ttgtcacagg ataacctggc agagatgatc cgccgctttg atgaaacggg
     4321 tcatagccag atcatggttg aaccggttgc tgatgtgacc gcatatggcg ttgtggattg
     4381 caaaggcgtt gaattagcgc cgggtgaaag cgtaccgatg gttggtgtgg tagaaaaacc
     4441 gaaagcggat gttgcgccgt ctaatctcgc tattgtgggt cgttacgtac ttagcgcgga
     4501 tatttggccg ttgctggcaa aaacccctcc gggagctggt gatgaaattc agctcaccga
     4561 cgcaattgat atgctgatcg aaaaagaaac ggtggaagcc tatcatatga aagggaagag
     4621 ccatgactgc ggtaataaat taggttacat gcaggccttc gttgaatacg gtattcgtca
     4681 taacaccctt ggcacggaat ttaaagcctg gcttgaagaa gagatgggca ttaagaagta
     4741 acatccgtat cggtgttatc cacgaaacgg cgttgagcaa tcgacgccgt ttttttatag
     4801 cttattctta ttaaattgtc ttaaaccgga caataaaaaa tcccgccgct ggcgggattt
     4861 taagcaagtg caatctacaa aagattattg cttgatcagg aaatcgtcga gggatttacc
     4921 ttgctcatcc attgcctttt tgattacagc tggagtacgg ccttggccag tccaggtttt
     4981 agtttcgccg ttttcgtcaa cgtagctata ttttgccgga cgctgagcac gtttagcttt
     5041 ggtgccagat ttaacggcag caaggctatt cagcagttcg ttcgggtcaa taccgtcagc
     5101 gatcagcatt tcgcgatatt gctgcagttt acgagtgcgc tcttcaactt cagcagcagc
     5161 cgcgctttct tcttcgcgac gttcgttaac aacaacttct aatttttcca gcatttcttc
     5221 cagcgtttca agtgtacatt ctcttgcctg cgcacgaaga gtacggatgt tgttcagaat
     5281 tttaagtgct tcgctcattg tagtaatctc aaacttatat tggggtggtt tgttgaggta
     5341 ataatagagc cttaaattca gttgtgcaat agccaggaat gtaaggaatt caaaattgtt
     5401 ctttattttg tgccgccaat aaatatcttt tcataaaatt agccagaaaa gacgcggcat
     5461 atagccctat ttgtaccgat gatttcgcag ctcgtgaggt taaaacttcc tgattcatgt
     5521 cacattttat ggggagatta tcgtaggctg acgacctttc agtcttctgt attagtcgtg
     5581 tttacgagaa ttccctatta agcgaatgat gaaaagtaga acagtcgcaa taagagcagg
     5641 gacttagtat tgcactatct cctggaggtc aacagagggc tattacttgc gcaacagatt
     5701 aaagattgtg aatagttacc agcagtcatt tacccgctta taacaagcga ggcagttgta
     5761 atgatagctc agaaggatta tgcaaggctt cgtaagggag aacgcatata ccacttctgt
     5821 gcatactgtt gagctgaaaa actgacgaat tatgataaac tccagccaac tttatttcat
     5881 atcattgagg gcctgtggct gatggcacag ctatatttct actattccgc aatgaatgcg
     5941 ggtaagtcta cagcattgtt gcaatcttca tacaattacc aggaacgcgg catgcgcact
     6001 gtcgtatata cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag ttcgcgtata
     6061 ggtttgtcat cgcctgcaaa attatttaac caaaattcat cattatttga tgagattcgt
     6121 tcggaacatg aacagcaggc aattcattgc gtactggttg atgaatgcca gtttttaacc
     6181 agacaacaag tatatgaatt atcggaggtt gtcgatcaac tcgatatacc cgtactttgt
     6241 tatggtttac gtaccgattt tcgaggtgaa ttatttattg gcagccaata cttactggca
     6301 tggtccgaca aactggttga attaaaaacc atctgttttt gtggccgtaa agcaagcatg
     6361 gtgctgcgtc ttgatcaagc aggcagacct tataacgaag gtgagcaggt ggttattggt
     6421 ggtaatgaac gatacgtttc tgtatgccgt aaacactata aagaggcgtt acaagtcggc
     6481 tcattaacgg ctattcagga aaggcatcgc cacgattaat aagaatttct ttactgacag
     6541 ggtgagcagg gcgcttttat cctgtcagtt cgttttactc acttcttccg ggctatacac
     6601 ccttctcggc agttttttaa cgccgctata cgcctcacag gacgcttaag caccgacgtt
     6661 gacttgtgac ctgtaaagta caatatccct gtgtttaggc gttatacatc gtcgcaaata
     6721 tgatgaaggc taatgctgtc ggtttatgga aaagttgctt tgggtaaaca aaaaaacggc
     6781 cccagaaggg caatgccgtt cacttaagag gagcggcact atgtttcaca ggataacggg
     6841 tttttgatat cttaaccgac ctcggccttg atggtcgggg gcgttttgtt atgaacacca
     6901 cttccagagc accccgaaga tgctccagtc gtttcgggat ggtccctggt gacgccgtgt
     6961 ttcccagctc tatcatttct gatgcgatat tcttccacgc aggcagtagc cagtggcggt
     7021 tacaaccctt ctggttcagc gtcagcagca ggtcttcgct gtaaaaaagt ttatcaaaca
     7081 acgtaataga gttatccggg atggtggcga gcatggagtg ggccagcaca gtttcgctct
     7141 gccggtaagg tgcggtcacg gcattcagca gaatgtgact tcccaggttc attaaggcca
     7201 ccagacgcat aaccgggtag gcgttctgcc gcttagtgga tgtgttggca gacccataat
     7261 attcacgcag ctcgggttta tcaggtgtcc tgaactgtgc gccatcaatg gcaaaaagtt
     7321 gcaggccgtg ccagtcatcc ttcaggtaac gttccgcgcc cctgtctgtg cggtctggcg
     7381 gaagagccat tccactgggg cggcccccac gcgctgacgc gcctgggtga cagcgctgcg
     7441 ggccagcagg ttcatccccg cttcgccatc cgcgctcagg ttcagacggc gaacaacatc
     7501 ggtaattggc tcattgcacc accatccaga taaccatgtc ccccggtaaa cgacggcggc
     7561 gaacggtcgc atgagcagaa agcgtcaggc agtgttgtat ccactcggtg ggaaggtgtt
     7621 ctgcaaatag ttgtgcagag ggcggaggca taagcggatg gtcactgaaa tcgagcagat
     7681 cattgagaag tggcataaga aaacggctcc ctgttgtgga agccgttata gtgcctcagt
     7741 ttaaggatcg gtcaactaat ccttaactga tcggcattgc ccagaagggg ccgtttatgt
     7801 tgccagacag cgctactgat taagcggatt ttttcgcttt tttctcagct ttagccggag
     7861 cagcttcttt cttcgctgca gtttcacctt ctacataatc acgaccgtag taggtatcca
     7921 gcagaatctg tttcagctcg gagatcagcg ggtaacgcgg gttagcgccg gtgcactggt
     7981 catcgaatgc atcttcagac agtttatcca cgttcgccag gaagtctgct tcctgaacgc
     8041 cagcttcacg gatagatttc ggaataccca gttcagcttt cagcgtttcc agccatgcca
     8101 gcagtttctc gatcttagca gcagtacggt cgcccggtgc gctcagaccc aagtggtcgg
     8161 caatttcagc ataacgacgg cgagcctgcg gacggtcata ctggctgaat gcagtctgct
     8221 tggtcgggtt gtcgttcgca ttgtagcgaa taacgttaca aatcagcagg gcgtttgcca
     8281 gaccgtgcgg aatatggaac tgggaaccca gtttgtgcgc cattgagtga catacaccca
     8341 ggaaggcgtt cgcaaacgcg atacccgcga tagtcgctgc actgtgaaca cgttcacgcg
     8401 ctaccggatt tttagaccct tcgtggtagg acgctggcag atattctttc agcagtttca
     8461 gtgcctgcag agcctgacca tcagagaact cagatgccag tacagaaaca taagcttcca
     8521 tggcgtgagt tactgcgtcc agaccaccga aagcacacag ggacttcggc atgtccataa
     8581 ccaggttggc gtcgacaatc gccatatccg gagtcagcgc atagtctgcc agcggatatt
     8641 tctgaccagt agcgtcgtca gttacaaccg caaacggagt gacttcagaa cctgtaccag
     8701 aagtggtggt gacagcgatc attttcgctt tcacgcccat tttcgggaac ttgtagatac
     8761 gtttacggat atccataaag cgcagcgcca gctcttcgaa gtgagtttcc ggatgttcgt
     8821 acataaccca catgatcttc gcggcgtcca tcggggaacc accacccagc gcgataatca
     8881 cgtctggttt gaaggagttt gccagttctg cacctttacg aacgatgctc agggtcgggt
     8941 ccgcttctac ttcgaagaag acttcagttt caacgcctgc tgctttcagt acggaagtga
     9001 tctgatcagc ataaccattg ttgaacagga agcggtcagt cacgatgagc gcacgtttgt
     9061 ggccatcagt aatcacttca tccagcgcga ttggcaggga gccacggcgg aagtagatag
     9121 atttcggaag tttgtgccac aacatgtttt cagctcgctt agcaacggtt ttcttgttga
     9181 tcaggtgttt cggaccaacg ttttcagaga tggagttacc accccaagaa ccacaaccca
     9241 gagtcaggga aggtgcgagt ttgaagttat acaggtcacc gataccaccc tgagacgctg
     9301 gggtgttaat caggatacgc gccgttttca ttttctgacc gaagtaagaa acgcgagccg
     9361 gttggttatc ctggtcagtg tacaggcaag aggtatgacc gataccgccc atagcaacca
     9421 gtttctctgc tttttctacc gcgtcttcga aatctttagc gcggtacatt gccagagtcg
     9481 gggacagttt ttcatgtgcg aacggttcgc tttcatcaac aacggtcact tcaccgatca
     9541 gaatcttggt gttttctggt acagagaagc ctgccagttc agcaatttta taggctggct
     9601 gaccaacgat agccgcgttc agcgcaccgt ttttcaggat aacatcctga acagctttca
     9661 gctctttacc ctgcaacaga tagccgccgt gggttgcaaa acgttcacgt acagcgtcat
     9721 aaacagagtc aacaacaaca acagactgtt cagaagcaca gattacgccg ttgtcgaagg
     9781 ttttggacat cagtacagat gcaactgcac gtttgatatc agcagtttca tcgataacaa
     9841 ctggagtgtt gcccgcgcct acaccgatag ctggtttacc ggagctgtat gcggctttaa
     9901 ccatgcccgg accaccagtc gcgaggatca ggttgatgtc tgggtggtgc atcagtgcgt
     9961 tagacagttc aacagaaggt tgatcgatcc agccgatcag atctttcgga gcaccggcag
    10021 cgatagcagc ctgcagaacg atatcagccg ctttgttggt ggcatctttt gcacgcgggt
    10081 gcggggagaa gataatggcg ttacgggtct tcagactgat cagcgatttg aagatagcag
    10141 ttgaagtcgg gttagtggtc ggaacgatac cgcaaataat accgattggt tcagcgatag
    10201 tgatggtacc aaaagtgtcg tcttcagaca gaacaccaca ggttttttca tctttatagg
    10261 cgttgtagat atattcagaa gcaaagtggt ttttgatcac tttatcttcg acgataccca
    10321 tgccggattc ggcaacggcc attttcgcga gtgggattcg agcatctgca gcagccagag
    10381 cggcggcgcg gaagattttg tctacttgct cttgagtgaa actggcatat tcacgctggg
    10441 ctttttttac acgctctacg agtgcgttaa gttcagcgac attagtaaca gccataatgc
    10501 tctcctgata atgttaaact tttttagtaa atcatctgct cgaatacgag agtatagtca
    10561 gtgcggtgat gatttgctta acctatgaaa atcaaaagct tactcgcgct cacactcact
    10621 gtgatttact aaaagagttt aaacattaga gttattatct ctaatgcgtc acttccaggt
    10681 ggcgtaagca agattactca cttctgggta ctgattacgt gatccaaatc aaatttttgc
    10741 aaagctgaca cctttcagca tcgcttttcg ccattatagc taacagttaa taaattgtag
    10801 tatgatttgg tggctacatt agcatgtttt gcacaactag ataacaataa cgaatgatag
    10861 caattttaag tagttaggag gtgaaaaatg ctgtcaaaag gcgtattgtc agcgcgtctt
    10921 ttcaacctta tttatggcta acattatccg gcttttgctt cggagctaac cgtgattcag
    10981 accttttttg attttcccgt ttacttcaaa tttttcatcg ggttatttgc gctggtcaac
    11041 ccggtaggga ttattcccgt ctttatcagc atgaccagtt atcagacagc ggcagcgcga
    11101 aacaaaacta accttacagc caacctgtct gtggccatta tcttgtggat ctcgcttttt
    11161 ctcggcgaca cgattctgca actttttggt atatcaattg attcgttccg tatcgccggg
    11221 ggcatcctgg tggtgacgat agcgatgtcg atgatcagcg gcaagcttgg cgaggataaa
    11281 cagaacaagc aagaaaaatc agaaaccgcg gtacgtgaaa gcattggtgt ggtgccattg
    11341 gcgttgccgt tgatggcggg gccaggggcg atcagttcta ccatcgtctg gggtacgcgt
    11401 tatcacagca ttagctatct gtttggtttc tttgtggcta ttgcattgtt cgctttatgt
    11461 tgctggggat tgttccgcat ggcaccgtgg ctggtacggg ttttacgcca gaccggcatc
    11521 aacgtgatta cgcgtattat ggggctattg ctgatggcat tggggattga atttatcgtt
    11581 actggtatta aggggatttt ccccggcctg cttaattaat tcctttcaaa tgaaacggag
    11641 ctgccatgct ccgtttactt cgtcattatt tttactttgt tcccgcgcag ttatcaaaag
    11701 caaaaggaat aggtaaaaat attcttctca aattacagtt agttataagg atttccttaa
    11761 ctgcttctcc tcaccatcat gttattttcg ccacatcata atcctgggct tgctgaagaa
    11821 taattgaaat gatattagta attccactgc ctttggtaga ggaaagtgct aaataataat
    11881 caattgttaa attattgtgc atttcactac tggaactgta atcagaaaag atagacatgc
    11941 ttagccaatc tctatttgat tgaattgaaa gatgtttgtt aaggcatgga tgcaagctat
    12001 agattctgat acggtcaata aaagagaatt gcttaacaat tttgcaaaat gtattggcga
    12061 gtaagaaccg catttggtac tttccgggca accgccagac gattctttat tggtaatgag
    12121 aataattaac aattaaagag cgtcgcgaaa gaataatgtg tctcgacagg ggagacacag
    12181 tacgaatcga cataaggtga tcgtctgaat caccagaata aataaagtcg gtgatagtaa
    12241 tacgtaacga taaagtaacc tgacagcaga aagtctccga gcctgtgcag ggtcccaatc
    12301 cgggattaca catgctggtt aataccagta attataatga gggagtccaa aaaacaatga
    12361 ccaacatcac caagagaagt ttagtagcag ctggcgttct ggctgcgcta atggcaggga
    12421 atgtcgcgct ggcagctgat gtacccgcag gcgtcacact ggcggaaaaa caaacactgg
    12481 tacgtaacaa tggttcagaa gttcagtcat tagatccgca caaaattgaa ggtgttccgg
    12541 agtctaatat cagccgagac ctgtttgaag gcttactggt cagcgatctt gacggtcatc
    12601 cagcacctgg cgtcgctgaa tcctgggata ataaagacgc gaaagtctgg accttccatt
    12661 tgcgtaaaga tgcgaaatgg tctgatggca cgccagtcac agcacaagac tttgtgtata
    12721 gctggcaacg ttctgttgat ccgaacactg cttctccgta tgccagttat ctgcaatatg
    12781 ggcatatcgc cggtattgat gaaattcttg aagggaaaaa accgattacc gatctcggcg
    12841 tgaaagctat tgatgatcac acattagaag tcaccttaag tgaacccgtt ccgtacttct
    12901 ataaattact tgttcaccca tcaacttcac cggtgccaaa agccgctatc gagaaattcg
    12961 gcgaaaaatg gacccagcct ggtaatatcg tcaccaacgg tgcctatacc ttaaaagatt
    13021 gggtcgtaaa cgaacgaatc gttcttgaac gcagcccgac ctactggaac aacgcgaaaa
    13081 ccgttattaa ccaggtaacc tatttgccta ttgcttctga agttaccgat gtcaaccgct
    13141 accgtagtgg tgaaatcgac atgacttata acaacatgcc gatcgaattg ttccagaagc
    13201 tgaaaaaaga gatcccggac gaagttcacg ttgatccata cctgtgcact tactattacg
    13261 aaattaacaa ccagaaaccg ccattcaacg atgtgcgtgt gcgtaccgca ctgaaactag
    13321 gtatggaccg cgatatcatt gttaataaag tgaaagcgca gggcgacatg cccgcctatg
    13381 gttacactcc accgtatact gatggcgcaa aattgactca gccggaatgg tttggctgga
    13441 gccaggaaaa acgtaacgaa gaagcgaaaa aactgctggc tgaagcgggt tataccgcag
    13501 acaaaccgtt gaccatcaac ctgttgtata acacctccga tctgcataaa aagctggcga
    13561 ttgctgcctc ttcattgtgg aagaaaaaca ttggtgtaaa cgtcaaactg gttaaccagg
    13621 agtggaaaac gttcctcgac acccgtcacc agggtacttt tgatgtggcc cgtgcaggct
    13681 ggtgtgctga ctacaacgaa ccaacttcct tcctgaacac catgctttcg aacagctcga
    13741 tgaataccgc gcattataag agcccggcct ttgacagcat tatggcggaa acgctgaaag
    13801 tgactgacga ggcgcagcgc acagctctgt acactaaagc agaacaacag ctggataagg
    13861 attcggccat tgttcctgtt tattactacg tgaatgcgcg tctggtgaaa acgtgggttg
    13921 gtggctatac cggcaaagat ccgctggata atacctatac ccggaatatg tacattgtga
    13981 agcactaatg gcaatacgtg gggcaggagt gtcctgctcc acggtgtctg atttttatcg
    14041 cattacagaa ggcacaggcc agaaggtagg gcaatgttaa aatttattct acgtcgctgt
    14101 ctggaagcga ttccgacgct atttattctt attactattt cgttctttat gatgcgcctc
    14161 gcgccgggaa gcccttttac cggcgaacgt actttaccgc cagaagtgat ggccaatatc
    14221 gaagcgaaat atcatcttaa tgatccgatc atgacacagt atttcagcta cctgaaacaa
    14281 ctggcgcacg gtgatttcgg tccatcgttt aaatataaag attattcggt caatgacttg
    14341 gttgcatcca gttttcccgt ttctgccaaa ctgggagccg cagcattttt ccttgcggta
    14401 atactgggtg ttagtgctgg cgttattgcc gcattaaaac aaaacaccaa atgggactat
    14461 accgtgatgg ggctggcaat gaccggggtt gttatcccca gttttgtggt tgcaccatta
    14521 ttagtcatga tatttgcgat cattttgcat tggctgccgg gcggtggctg gaatggtggg
    14581 gcgcttaaat tcatgatatt gccgatggtg gcgttgtcac tcgcttatat cgccagtatt
    14641 gcgcgtatta cccgtggctc tatgattgaa gtattacact ccaattttat tcgtactgcc
    14701 cgggcgaaag ggttacctat gcggcggatc attttacgtc acgcattaaa acctgctctg
    14761 ttacccgtgc tctcttatat gggccccgca tttgtcggca ttattaccgg ttctatggtt
    14821 atcgaaacca tttatggttt gccggggatt ggacaactgt tcgttaatgg cgcattgaac
    14881 cgtgactatt ccttagtgtt aagcctgacc atcctggttg gtgctttaac cattttgttt
    14941 aatgccattg tcgatgtgct atatgcggtt atcgacccga aaatccgtta ctgatactgg
    15001 agctcgcgat gatgttaagt aagaaaaaca gcgagacgct ggaaaatttc agtgaaaagc
    15061 tggaggtcga agggcgcagc ttgtggcagg acgcacgtcg acgttttatg cataaccgtg
    15121 cggcggttgc cagtctgata gtgctggtgc tgatcgcgtt atttgtaatc ctggcaccga
    15181 tgctttccca gtttgcctat gacgatactg actgggcgat gatgtccagc gccccggata
    15241 tggagtccgg tcactacttt ggtactgact catccggtcg cgacctgctt gtgcgcgttg
    15301 cgattggcgg gcgtatctca ctcatggtcg gtgttgctgc ggcactggtg gctgtggtcg
    15361 tcgggacact ttatggttcg ctttccggtt atctgggcgg taaagtggat tcggtaatga
    15421 tgcgtctgct ggaaatcctc aactccttcc cattcatgtt cttcgtcatt ttgctggtga
    15481 cctttttcgg tcaaaacatc ctgctgattt tcgtggcgat aggcatggtt tcctggctgg
    15541 atatggctcg tattgtgcgt gggcaaaccc tgagtctgaa gcgcaaagag tttattgagg
    15601 cggcacaagt tggcggtgta tcgacatcgg gcattgttat tcgccacatt gtgccgaatg
    15661 tactcggtgt ggtggtggtc tacgcatcac ttctggtgcc cagcatgatc ctctttgaat
    15721 ctttccttag cttcctgggg ttgggtacgc aagagccgtt aagcagctgg ggtgcattgc
    15781 tgagtgatgg cgcgaactcg atggaagtct ctccatggtt attgttgttc ccagcgggat
    15841 tcctcgtggt gacgctgttt tgtttcaact ttatcggcga tggcttgcgt gatgccctcg
    15901 acccgaaaga tcgttaagga gtgcagccat gagcgtaatt gaaactgcaa ctgtgccgct
    15961 cgcacaacaa caggctgacg cactgctgaa cgtgaaagat ttgcgtgtca cctttagtac
    16021 cccggacggc gacgtcacgg cggtcaatga tttgaatttt tccctacgtg ccggagaaac
    16081 gctgggtatt gtaggtgagt ctggttcggg taaatcgcaa actgcatttg cgttgatggg
    16141 cctgttggct gccaacggac gtattggcgg atcggcaacc ttcaatgggc gtgaaatcct
    16201 caatttgcca gagcatgaac tcaataaact gcgcgctgaa caaatctcaa tgatttttca
    16261 ggacccaatg acttcgttga atccctatat gcgcgtcggt gagcagttaa tggaagtgct
    16321 gatgctgcat aagaacatga gcaaagctga ggcgtttgaa gagtcggtgc ggatgctcga
    16381 tgcggtaaaa atgccggaag cgcgtaaacg catgaaaatg tacccgcacg aattttctgg
    16441 cggcatgcgt cagcgagtca tgattgcgat ggcattgcta tgtcgaccta agctactgat
    16501 tgcggatgaa cccactactg cgctggacgt caccgtacag gcgcagatca tgacgctatt
    16561 aaatgaactg aagcgggaat ttaataccgc catcattatg atcacccacg atcttggcgt
    16621 ggtggcgggg atctgcgaca aagtgctggt aatgtacgcc gggcgcacga tggaatatgg
    16681 caatgcgcgc gatgtctttt atcaacccgt tcatccttat tctatcggtt tgctcaacgc
    16741 ggtgccgcgt cttgatgcgg aaggtgaaac aatgttgacc atccctggta atccgccaaa
    16801 cttgctgcga ttaccgaaag gttgcccgtt ccagccacgt tgtccacatg cgatggaaat
    16861 ttgtagtagc gcaccgccgc tggaagagtt tacgcctggc cgtctgcgtg cttgctttaa
    16921 accggtggag gaactgttat gaatgctgta actgaaggaa gaaaagtcct ccttgaaatc
    16981 gccgatctta aagtgcactt tgaaatcaaa gatggcaaac agtggttctg gcaaccgccg
    17041 aaaacgctca aagccgtcga tggtgtcact cttcgcctgt atgaagggga aacattaggt
    17101 gtggtagggg aatcgggatg cggtaagtcc acctttgctc gcgccatcat cggtttggtc
    17161 aaggcgaccg acggtcatgt tgcctggtta ggtaaagagt tgctgggcat gaagcccgat
    17221 gaatggcgtg ccgttcgcag tgatattcag atgattttcc aggatccgtt ggcatcgcta
    17281 aacccgcgta tgaccatcgg cgagatcatc gctgaaccac tgcgtactta tcatccgaaa
    17341 atgtcacgcc aggaagttcg cgagcgcgtg aaggcgatga tgctgaaagt cgggttattg
    17401 cctaacctga ttaaccgcta tccgcatgag ttctccggtg ggcagtgcca gcgtatcggg
    17461 attgctcgtg ctcttattct tgaaccgaag ctgattatct gcgatgagcc ggtgtcggcg
    17521 ctggacgtgt caattcaggc gcaggtggtc aacctgctcc agcagctgca acgtgagatg
    17581 ggattgtcat taatttttat cgctcatgac ctggccgtgg taaaacacat ttccgatcgt
    17641 gtgttggtga tgtatctcgg ccatgcggta gaactgggga cctatgatga ggtctaccac
    17701 aatccactac atccttacac cagggcattg atgtcggcag tccccatacc tgatccggat
    17761 ctggagaaga acaaaaccat ccagttactg gaaggggaat taccgtcgcc gatcaacccg
    17821 ccttccggtt gtgttttccg tacccgttgc ccgattgccg gtccggagtg cgccaaaaca
    17881 cgtcctgttc tggaggggag tttcagacac gccgtttctt gcctgaaagt cgatccgctt
    17941 taaacacaat aagggctgac agttgtcagc ccttattcac gccgaaagcg attatttatt
    18001 cccgccagat gatatggcaa agtttgtgat ctttttcgcg gcataacaga atgcgggcga
    18061 aaacatcgtt gatttcacca tcttcactgt ccgccagacc aatcaccact tcggcaaaaa
    18121 agtccgggtt caaatcgaaa tcaacatgtt cctgccagtc ttccgccgga tcgaataact
    18181 ccgcgccgcc gcgctcttca aactgaagat tgaacagcag aacatcagct ggatcgagat
    18241 tgtcggcagc cagttcgaga aaaatatcgt aggcctgctc aagcgtttca tcttcagtca
    18301 ggcgattgtt tagatccata tccatagtca ctacctgttt aacctctgtt ggcgacgttt
    18361 tacagcaacg ggctgaagaa gtaaaacagt cgctcggcga cacgttgcca taatggacgt
    18421 tttagccata aacgggcatc gagcagacgt gaacgcgaaa tataatcgtc ctgaacggcg
    18481 gcaaggtctg caccaaaacc tttatcgtcg attgccagag taatctcgaa atttagccac
    18541 agactccgca tatcaaggtt aacggtgcca accagactta gttcaccatc gaccagcacg
    18601 ctcttggtat gcagtaaccc gccttcaaac tgataaattt taaccccagc agccagcagt
    18661 tccgtaaaga atgcgcgact ggcccagccg accagcatcg agtcattttt tcgcggaagg
    18721 ataatactga catccacccc gcgctgtgcc gccgtgcaaa tcgcatgaag taaatcatcg
    18781 cttggcacaa agtagggcgt tgtcatgatc aaatattcac gcgccgaata agccgcagtc
    18841 aataatgcct ggtgaatgag atcttccgga aagcctgggc cagaagcaat tgtgtgaatg
    18901 gtgtgaccgc tggcctgttc aaacggcata atattgacat ctggtggtgg cggcagaata
    18961 cgttttccgg tttcaatctc ccagtcgcag gaataaataa tccccatcgc ggtggcgatg
    19021 gggccttcca tacgcgccat cagatcaatc cattgcccta cgcccgcatc ttgtttgaag
    19081 tagcgagggt cgaccatatt catgctgccg gtgtacgcga tgtaattatc gatcatgatc
    19141 atcttgcgat gttggcgcag gtccatacgg cgtaaaaaca cacgcatcag attgaccttt
    19201 aaggcttcga ccacttcaat accggcatta cgcattagct cgggccacgg gctgcggaaa
    19261 aaagccacac tcccggcgga gtcgagcatc aatcggcaat gaatgccgcg tcgcgcagca
    19321 gccattaatg attcagccac ttggtccgcc atgccgccgg gctgccagat ataaaacacc
    19381 atctcaatat tatggcgcgc gagctggatg tcgcggatta acgcctgcat cacatcatct
    19441 gactcggtca tcagttgtag ctgattccct ttgaccccag cgatcccctg acgacgctcg
    19501 caaagcttga ataatggcgc agcgacactg ctattttctt cggcgaagat atgcttacag
    19561 gctttaaggt cgttaagcca ttttgcggtg gaaggccaca tcgctctggc gcgctcagcg
    19621 cggcgtttgc ctaaatggag ctcgccaacg gcaagatagg caataattcc gactaacggc
    19681 agaatgtaaa taatcaacag ccaggccatc gcggagggaa ctgcgcgtcg tttcattaga
    19741 atgcgtaaag ttacgcctgc aatgagcaac cagtatccca gaatggccaa ccaactcacc
    19801 aacgtataaa cggttgtcat agattagaaa tccttttgaa agcgcattgt tatgagttta
    19861 cgcatatgga ttcatctggc aaataaaaac gcgggaaaag cactggtcag cgtcgggggc
    19921 gggggtataa tgaccattct gttattgcat agagtagtta acatgaagcg gagtagaacg
    19981 gaagtggggc gctggcgcat gcagcgtcag gctagccgac gtaaatcgcg ttggcttgag
    20041 gggcaatcgc gccgaaatat gcgtatccac agcatcagga agtgcattct aaacaaacag
    20101 cgtaactcgt tattgtttgc gatctacaat atctaaatgt actgggcacc attgcggtgc
    20161 ccaataagtt tactcctccg tctttaaact ctatttttgc gccgattctt tactgtcagc
    20221 tttcgtttct tgttcatcgt tatcgctgaa aataccatga cctgagttca acaacattga
    20281 aacgagcata tcattattaa tctcttcacc atttaaaact cgcgccagaa tttcgctgcc
    20341 aaacagttgc ggtgagagaa tgatatccgg atgtaccatc ttaatcttat tcaggttttt
    20401 gctatcactg acggcgagaa ctgttttgac atcactgctc atatcttttg ccgagagtac
    20461 aacaaacgcg ttatctgcat cgttatcact cagcgccaga atggcccggc aacgatcgat
    20521 tcccgctttc tttaatacag aactgtcatt actgtcaccg gggataacat cagcattatc
    20581 gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc aagttgctga taaccgttac
    20641 gttttgtccg cgttgattca gttgcaggat cgtattgatg gcgagaatcg aatgtccgca
    20701 aacaataaaa tgatctttac gatgcattgt atgattgttt ccttttacaa gtttgttgaa
    20761 tcccccgcgg ataagcggac caaaaattga ggtcatagat gtggcaaaaa cggtaatgcc
    20821 ggaaataatg accgaaatag tgaacaatcg tgctgattca gaaacaggga caatatcgcc
    20881 atagccgacg gttgacatgg tttctatcga aaaatagaac gcggtcatca aactttctat
    20941 tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag gttgagtaaa acagtaatgt
    21001 cgtgaaacta ataaatgcaa aaattgtccc ggctgcggcg ctactgtggg agaagtcttt
    21061 gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat ccaatactaa atttcagcca
    21121 gggataaaaa tgcagggtgt aaattagcgc tatcaacaac aaaatgatac tgattgccca
    21181 tgcgagcttt gcccggaaca gtaagccaat ggagttcagg acaagaaaga cgccgagcat
    21241 aaagagtggg gcgtttgcca gcaaggagag atccaactct gaaaatgctt tgatatggaa
    21301 aatatcaagc aaattgaccg acataccata gattgtttta aaaataagca atccatttaa
    21361 aaagacggcc agcgcgagaa tgtcgtgccg aagggtcacc cataaatttg ttgctgtttg
    21421 tttgaatgta gcccagtgac tcactgatac ctctgtaacc acaattttgt ttaattatct
    21481 tagcgtgcac attttctgaa catacatgca gcgcgtgaat gtgttaaaca atagtagatg
    21541 actttatccg cagtaatcaa aggatttgag cgagcaactg tacctcagga aaggtcattt
    21601 ttcctgaata tgctcacatc atataaagaa atacagataa agttattatc tgcttgtggt
    21661 ggtgaatgca ctgaccggct ataaggaaag gccaaacaag aacacggttg caaaaaccgt
    21721 gcccttaaat attgaatctc tattcagaac actttcttaa atggtttcac tgaaacgtgt
    21781 tcatagactc ctgccgctac gtacgggtca gcatcggccc aggcctgagc tgcttccagc
    21841 gattcaaatt cagcaataac ggttgagcca gtaaatcccg cagcccctgg atcgttactg
    21901 tctaccgctg gcattggacc agctgtcaac aaacgacctt catcatgcag taactgtaaa
    21961 cgtgctaaat gtgccggacg aacggaaagg cgtttttcga ggctatcagc tttgtcttga
    22021 gcgtaaataa catacaacac gggcacaact ccttgttcgg gaaagttgta aagtacgtta
    22081 tttgaaaggg cgaagatctg caacggaaag atgatgtctt tgttaaggcc atgcataaag
    22141 taagggtaat tacgccaaaa atgacatttt cactgatcct gatcgtcttg ccttattgaa
    22201 tatgattgct atttgcattt aaaatcgaga cctggttttt ctactgaaat gattatgact
    22261 tcaatgaccc ttgaataaag tctattcggt tttctttaga tgttcgcctc acataatctt
    22321 caagtgatgt tccttacatc tccctagagc gttaagatag ttaactgatt gagttttcag
    22381 ggtatgaaag gatgagcaac actactgatt ggggaagcat gatagccaac atagttatgg
    22441 ctactaccgc tgtctgggct gcattcaaag ctaatgactg gtttaaaacc aagcgtcttg
    22501 agagtgctga aaacttctat aaagaagtac acaatctcca taaacgttac tattcagcta
    22561 tccaatacgt tgaggattca agaagaatat ttgatagtca gtatgggttg cattcgccaa
    22621 tatctgaacc taaagacaag tcattgcaat gggaggttaa atcgaactct tacaaaatca
    22681 tcgctgaact agattatctg gcatcgtcac tacaaatgat ggatgcaatg ggtaacaaac
    22741 ttagccctaa atcggcttca ctggttagtg atatgatgga agcacatgga cgattctgta
    22801 atgatgtgat gaacttcaat gactcagttt ggaaggttat aagggcaggt ataactgaag
    22861 agaacatccc acatacgcga ggctatgtga acggcgatta catggctatc cttaccagta
    22921 tggatgaacc tgtaagaata tcttttaagt taaaagaaat cagttttaaa gatttttact
    22981 tacatgaaac tactatgcag tcagtaataa cgaggctgaa atcgcttatc gccagaacag
    23041 g
//
LOCUS       DACAPB010000058        22871 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.058, whole genome shotgun sequence.
ACCESSION   DACAPB010000058 DACAPB010000000
VERSION     DACAPB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.058"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            93..290
                     /locus_tag="IT904_002044"
     CDS             93..290
                     /locus_tag="IT904_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191827.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            1706..2038
                     /locus_tag="IT904_002045"
     CDS             1706..2038
                     /locus_tag="IT904_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191828.1"
                     /translation="MKGKNIALEKLASFPDSADIYHAIHLSFDIWVTCPLTSPDDIKN
                     FRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLKELSRVRRLSTGEMLHDLIVEE
                     YKRYRHSR"
     gene            2123..2995
                     /locus_tag="IT904_002046"
     CDS             2123..2995
                     /locus_tag="IT904_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP3191829.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            3366..6212
                     /gene="ag43"
                     /locus_tag="IT904_002047"
     CDS             3366..6212
                     /gene="ag43"
                     /locus_tag="IT904_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP3191830.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAVALSLAA
                     VTSVPVLAADTVVQAGETVSGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGHLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNDATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSTQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            6283..6441
                     /locus_tag="IT904_002048"
     CDS             6283..6441
                     /locus_tag="IT904_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP3191831.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            6596..7414
                     /locus_tag="IT904_002049"
     CDS             6596..7414
                     /locus_tag="IT904_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP3191832.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEYRPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            7756..8229
                     /locus_tag="IT904_002050"
     CDS             7756..8229
                     /locus_tag="IT904_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP3191833.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            8245..8721
                     /locus_tag="IT904_002051"
     CDS             8245..8721
                     /locus_tag="IT904_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP3191834.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            8784..9005
                     /locus_tag="IT904_002052"
     CDS             8784..9005
                     /locus_tag="IT904_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP3191835.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            9168..9536
                     /locus_tag="IT904_002053"
     CDS             9168..9536
                     /locus_tag="IT904_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP3191836.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            9626..10003
                     /locus_tag="IT904_002054"
     CDS             9626..10003
                     /locus_tag="IT904_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP3191837.1"
                     /translation="MKTLPDTHVRELSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            10000..10203
                     /locus_tag="IT904_002055"
     CDS             10000..10203
                     /locus_tag="IT904_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191838.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLAELINKVAENGDS
                     LRVVDERDSTETPATYASPHQLL"
     gene            10216..10329
                     /locus_tag="IT904_002056"
     CDS             10216..10329
                     /locus_tag="IT904_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP3191839.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNQ"
     gene            10488..11516
                     /locus_tag="IT904_002057"
                     /pseudo
     CDS             10488..11516
                     /locus_tag="IT904_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216294.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            11787..11957
                     /locus_tag="IT904_002058"
                     /pseudo
     CDS             11787..11957
                     /locus_tag="IT904_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
     gene            11970..12152
                     /locus_tag="IT904_002059"
     CDS             11970..12152
                     /locus_tag="IT904_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAP3191840.1"
                     /translation="MNHPVTGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALQHTEEGCC"
     gene            complement(12253..12582)
                     /locus_tag="IT904_002060"
     CDS             complement(12253..12582)
                     /locus_tag="IT904_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAP3191841.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(12754..13812)
                     /locus_tag="IT904_002061"
     CDS             complement(12754..13812)
                     /locus_tag="IT904_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP3191842.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFVNTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(14010..14483)
                     /gene="sbmC"
                     /locus_tag="IT904_002062"
     CDS             complement(14010..14483)
                     /gene="sbmC"
                     /locus_tag="IT904_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HAP3191843.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVQKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGDWDIEMYVAVQPKHH"
     gene            complement(14602..15768)
                     /gene="dacD"
                     /locus_tag="IT904_002063"
     CDS             complement(14602..15768)
                     /gene="dacD"
                     /locus_tag="IT904_002063"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HAP3191844.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIQAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDALGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            15977..17404
                     /gene="sbcB"
                     /locus_tag="IT904_002064"
     CDS             15977..17404
                     /gene="sbcB"
                     /locus_tag="IT904_002064"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAP3191845.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDANRLGINRQHCLDNL
                     KILRENPQVREEVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(17447..17674)
                     /locus_tag="IT904_002065"
     CDS             complement(17447..17674)
                     /locus_tag="IT904_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAP3191846.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(17688..18746)
                     /gene="yeeE"
                     /locus_tag="IT904_002066"
     CDS             complement(17688..18746)
                     /gene="yeeE"
                     /locus_tag="IT904_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAP3191847.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQYYSTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(18925..20283)
                     /gene="plaP"
                     /locus_tag="IT904_002067"
     CDS             complement(18925..20283)
                     /gene="plaP"
                     /locus_tag="IT904_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAP3191848.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(20273..20335)
                     /gene="yoeI"
                     /locus_tag="IT904_002068"
     CDS             complement(20273..20335)
                     /gene="yoeI"
                     /locus_tag="IT904_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAP3191849.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(20550..21479)
                     /locus_tag="IT904_002069"
     CDS             complement(20550..21479)
                     /locus_tag="IT904_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3191850.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(21525..22349)
                     /locus_tag="IT904_002070"
     CDS             complement(21525..22349)
                     /locus_tag="IT904_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3191851.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(22432..22686)
                     /gene="yoeB"
                     /locus_tag="IT904_002071"
     CDS             complement(22432..22686)
                     /gene="yoeB"
                     /locus_tag="IT904_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAP3191852.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(22683..>22871)
                     /gene="yefM"
                     /locus_tag="IT904_002072"
     CDS             complement(22683..>22871)
                     /gene="yefM"
                     /locus_tag="IT904_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAP3191853.1"
                     /translation="EDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLM
                     DSIDSLKSGKGTEKDIIE"
BASE COUNT         5902 a         5929 c         5634 g         5406 t
ORIGIN      
        1 ttcgtcgtca tcatactgag ccatgacggg cgtcttaagg gcatcctgtc attgctgagc
       61 aggcaacacc accagaggag ccacggacag ggatgttctt tacagcccga tacacgccag
      121 gttctcagca gactgaatgt gcaggtgcgg ttattcacca gaaatggcat gctgcggtac
      181 atacccatcc tggtgacaat atgtacgtgg cgacagcgat cccggcactg tggaagagac
      241 tcactcagcc tgcaggatat tctttccggc atccgggaat gtccgcctga atgcaaacag
      301 gatgatattt gaatccgtaa tactgttctt tcatcataaa taatttatgc agatacagtt
      361 aaccgttaca gtagccggtt tgttttaccg ggaaataata tccgtcagac tgatgccgga
      421 acatatctga aacacgttat gatgttttgt tatcagaaat gttgtatatt cttatccgtg
      481 gcgacagttt aatgccctct ggtatcacct gataatgcat atcctgcgat gattcgttat
      541 atttacggga cacgcctctt ttcattgtgc tccttttcag acttcatttt ccgtaagtgt
      601 tccccggcat aaaggaggta cctttctggc gtttataact ctctttatcg gtgacgccgg
      661 taatacaggg aataccacac cgacaaacga ggtattaaca gacgatattt cagtgtccat
      721 aatttttctg tccggttaaa taacgtcagt ctgctgccct ctgactgtaa tcatcaaagc
      781 cggaatgcat accaggttac aacgtaaaaa aagtgatgct ggcccccggt gcagctggcg
      841 ggcgaaacat gtttaccttt caatacccct tgtctgcagt ctccactctt ccatcctctt
      901 aagctacgtc ggccacatga gtgcacttcc atgataacag gtattcctgt cagcataacg
      961 aagccggtta ccttcccttc ctcctgcatt tcctgtcaga atgactatac agcgcgcaga
     1021 ttgccttttt attgaccatc ttaatatacc attataaact tctttttatc atgttattat
     1081 caccttatta tgtttaattt ttaagcagcc ggagaaagag gatggttcag aaaattttgt
     1141 cggataaggt tatgaatgaa agaacaaatg cgtattactc ttattatctt ggagagcgta
     1201 atatctccgt actgcccctt aatgtttatg atcccccgga acgctttatc gcttacataa
     1261 aaaaaacaga gaaaatctga acatcacact ttcagatttt gaacttgaac aaatcatttc
     1321 tggtatgcgt ctgaaggcac tggcctccct ggtgcccctg gaaaaaatat cctggatcgc
     1381 gggcagtgaa cgtgcatgct tattttcctg gtatctgttg atgcagttta tccagaataa
     1441 caggacaaaa ataagcgcag acttgcttca aaagaataaa ctgtatctta aagaagagta
     1501 tttagaaggg aatgccttcc cgtcggactc atcgacacag ttcagacaaa tactcagagt
     1561 tctcgatatt ctttctgata agaatttgcg ggatgagtgg ataatccaga ccaaagacag
     1621 atggatacgg gcatttaaat caaagagtcc tttcagttac ctcctcccgg agaacgaaca
     1681 tgaatgcata tggacatgga attatttgaa gggaaaaaat attgcactgg aaaaactggc
     1741 cagttttcct gattctgctg atatttacca cgcaattcac ctttcatttg acatatgggt
     1801 tacctgtcca ctcacctcac ctgacgatat aaaaaatttc agaaacagtt tcaataaggc
     1861 caaggcgcag cgcaagtaca aaaaaatgca ggaagataaa gtcaatgtac agttttttct
     1921 tgacgcagaa accagggcgc agctcaaaga attatcgagg gtcagaagac tcagtaccgg
     1981 cgaaatgctg catgacctga ttgttgaaga gtataaaagg taccgacatt ccagataatt
     2041 tcagacatat attatctccg tgagtccccc gccacctttc cggtgtgcgg ggtttttgtc
     2101 ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa ttgaaaaacc
     2161 gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc gcaaactcac
     2221 cagtcacgaa cccgtgattg gcattatggg taaaagcggg gccggtaaat cctcactctg
     2281 taatgcactg tttcaggggg aggtcacccc ggtcagtgat gttcacgccg gcacccggga
     2341 agtgcggcgc ttccgtctga gtggccacgg tcacagcatg attatcactg acctgcccgg
     2401 ggtgggcgag agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg acattctgcc
     2461 tgaacttgac ctggtactgt ggctgattaa agccgatgac cgtgccctgt ctgtggatga
     2521 gtatttctgg cgacacatcc tgcaccgggg acatcagcag gtgctgtttg tggtgacgca
     2581 ggccgacaaa acggagccct gccatgaatg ggatatggcc ggcattcagc cttctcccgc
     2641 acaggcacag aacattcggg agaaaacgga ggcggtattc cgtctgttcc ggcccgtaca
     2701 tccggttgtt gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg tcagtgcgct
     2761 aatgacagca cttcccgacc atgccgccag tcccctgatg acccgcctgc aggacgagct
     2821 gcgcacggaa tctgtccgcg ctcaggcccg tgaacagttt accggtgcgg tggaccggat
     2881 atttgacacg gcggagagcg tctgtgttgc ctctgttgcc cgtacggttc tgcgtgccgt
     2941 ccgtgacacg gtggtctctg ttgcccgcgc tgtatggaac tggattttct tctgaacagt
     3001 ctgccaggga aatgcctgat aacattacgt tatccaactg aaaacatgac acaaacccct
     3061 catactcctc ctgtttctct tgcgtgaccg ccttgtaatc aatagaataa aacgatcgat
     3121 aaaacaggta tcgatcgttg atatcgatcg tttatagtgg ttaatggcct gcgaaaaggc
     3181 agttgaatct cttcatcatg cagaacggaa ttgcacacaa cggactgata cttctctggc
     3241 ggatgagaga ggggaagcat ttatgtccgg gaaaaccact ttatctgacc atgctgtttt
     3301 gtacctgccg gtatccacat ttgtgggtac cggctttttt attcaccctc tgtaaggaaa
     3361 agctgatgaa acgacatctg aacaccagct acaggctggt atggaatcac attacgggca
     3421 ccctggtggt ggcttccgaa ctggcgcgct cacggggaaa acgcaccggt gtggcggttg
     3481 cgctgtctct tgctgctgtc acgtcagtcc cggtactggc tgctgacacg gtcgtacagg
     3541 cgggagaaac cgtgagcggc ggaacactga caaatcatga caaccagatt gtcttcggta
     3601 cggccaacgg aatgaccatc agtaccggtc tggagtatgg gccggataac gaggccaata
     3661 ccggcggaca atggatacaa aatggcggta tcgccaacaa cactactgtc accggtggtg
     3721 gtcttcagag agtgaatgcc ggaggaagcg tttcagacac ggttatcagt gccggaggcg
     3781 gacagagcct tcaggggcag gcagtgaaca ccactctgaa cggcggtgag cagtgggtac
     3841 atgaaggcgg gattgcaacg gttaccgtca ttaatgagaa gggctggcag gccgtcaaat
     3901 ccggcgcaat ggcaaccgac acggttgtga ataccggcgc ggaaggggga ccggatgcag
     3961 aaaatggtga taccgggcag accgtctacg gagatgccgt acgcaccacc atcaataaaa
     4021 atggtcgtca gattgtggct gctgaaggaa cggcaaatac cactgtggtt tatgccggcg
     4081 gcgaccagac tgtacatggt cacgcactgg ataccacgct gaatgggggg taccagtatg
     4141 tgcacaacgg cggtacggcg tctggcactg ttgtaaacag tgacggctgg cagattatca
     4201 aggaaggtgg tctggcggat ttcaccaccg ttaaccagaa aggcaaactg caggtgaacg
     4261 ccggtggtac cgccacacat gtcaccctga aacagggtgg agcactggtc accagtactg
     4321 cggcaacggt tcttggcagc aaccgtctgg gcaatttcac ggtggaaaac ggtaaggctg
     4381 acggcgttgt tctggagtcc ggcggtcacc tggatgtact ggaaggccat tcagcgcaga
     4441 aaacacgggt ggatgacggc ggaaccctgg cagtgtctgc cggtggtaag gcaacaggtg
     4501 ttaccatgac atccggtggt gccctgattg cagacagtgg tgccactgtt gaggggacca
     4561 atgccagcgg taagttcagt attgatggca tatccggtca ggccagcggc ctgctgctgg
     4621 aaaatggcgg cagctttacg gttaatgccg ggggacaggc cagcaacacc actgtcggac
     4681 atcgtggaac actgatgctg gctgccgggg gaagtctgag tggcagaaca cagctcagta
     4741 aaggcgccag catggtactg aatggtgatg tggtcagtac cggcgatatt gttaacgcag
     4801 gggagattta ctttgacaat cagacaacac cggatgccgt actgagccgt gctgttgcaa
     4861 aaggcaacgc cccggtaacg ttccataaac tgacaaccag taacctcacc ggtcagggtg
     4921 gcaccatcaa tatgcgtgtt cgccttgatg gcagcaatgc ctctgaccag ctggtgatta
     4981 atggtggtca ggcaaccggc aaaacctggc ttgcgtttac aaatgtcgga aacagcaacc
     5041 tcggggtggc aaccaccgga cagggtatcc gggttgtgga tgcacagaat gacgctacca
     5101 cagaagaagg tgcgtttgcc ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc
     5161 tgaaccgtga cagcgatgaa gactggtacc tgcgcagtga aaatgcttat cgtgctgaag
     5221 tccccctgta tacatccatg ttgacacagg caatggacta tgaccggatt ctggcaggct
     5281 cccgcagcca tcagaccggt gtaaacggtg aaaataacag cgtccgtctc agcattcagg
     5341 gcggtcatct cggtcacgat aacaacggcg gtattgcccg tggagccacg ccggaaagca
     5401 gcggcagcta tggcttcgtc cgtctggagg gtgacctgct cagaacagag gttgccggta
     5461 tgtctctgac gacaggggtg tatggtgctg caggccattc ttccgttgat gttaaggatg
     5521 atgacggttc ccgcgccggc acggtccggg atgatgccgg cagcctgggc ggatacctga
     5581 atctgacaca cacgtcctcc ggcctgtggg ctgacattgt ggcacaggga acccgccaca
     5641 gcatgaaagc gtcatcggac aataacgact tccgcgcccg gggctggggc tggctgggct
     5701 cactggaaac cggtctgccc ttcagtatca ctgacaacct gatgctggag ccacaactgc
     5761 agtacacctg gcaggggctc tccctggatg acggccagga taacgccggt tatgtgaagt
     5821 tcgggcatgg cagtacacaa catgtgcgtg ccggcttccg tctgggcagc cacaacgata
     5881 tgacctttgg cgaaggcacc tcatcccgtg acactctgcg cgacagtaca aaacaccgtg
     5941 tgagtgaact gccggtgaac tggtgggtac agccttctgt tatccgcact ttcagctccc
     6001 ggggtgacat gagcatgggt acagccgccg ccggcagtaa catgacgttc tcaccgtccc
     6061 ggaatggcac gtcactggac ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca
     6121 ccctgggcgt tcaggccggt tatgcccaca gcgtcagcgg cagcagcgct gaaggttata
     6181 acggtcaggc cacgctgaat gtgacttttt gataatcagg tcactctcct tcaggccccg
     6241 gtcatcatga ccggggcttt ttactttcag ggagcaacag atatgccggg ttgtacatca
     6301 cgtttattgc ccgaaggccc tttctcacgc gaacaggctg ttgccgtcaa aacagcttac
     6361 cgcaatgtgt ttactgaaga tgaccaggga acatattccc ggctggttat ccgtaatgct
     6421 gaaggtcagt tgcgctggtg atgccggaac acagcttaat actcagctcg ccagttaagg
     6481 gcttctcaga caataaacgt cattcatttc atctgtcagg ccgcgtcttc tccgggagac
     6541 gtggcttttt catttattac accagtaaat cgtaaccacc gataaggagc aaattatgcg
     6601 actagccagt cgttttggtt atgcgaacca gatacgccgt gaccgtccgc tgacacacga
     6661 agaactgatg cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa
     6721 acgatatgcg tacattccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca
     6781 gccattcttc gcctgccaga cccgtgtgcg cgacccgggc cgccggggat acacaaaaca
     6841 catgctgcgt ctgcggcggg ccggagagat aaacggagaa catgtccctg aaatcattct
     6901 gctcaactcc catgacggta cctccagcta ccagatgctg ccgggttact tcaggttcgt
     6961 ctgtcagaac ggatgcgtct gtggtcagtc tctgggggaa gtgcgtgttc cgcatcgggg
     7021 aaatgtggtg gacagagtca ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat
     7081 agaggagaag cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct
     7141 ggcacaggcg gcactgactt accgttatgg tgacgaatat cggcccgtca ccaccgccga
     7201 cattctgacg ccacgacgcc gggaggatta cggtaaggac ctgtggagta cttatcagac
     7261 catccaggag aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat
     7321 ccacacccgt gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt
     7381 aatggcagaa acgctgctgg agagcctgcg ctgatgcagc atgtccctga aggcgtgatt
     7441 caggcaccag aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat
     7501 gatgacctgt gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc
     7561 agactgagta tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt
     7621 acggctgtcc ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg
     7681 tcagtgatga acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt
     7741 acaaggattt catacatgaa aacattgtct cagaacacca cctcttcggc ctgtgcaccg
     7801 gagactgacc tgcaacaact ggttgccacc atagtccctg atgaacagcg catcagcttc
     7861 tggccgcaac actttggcct cattccacag tgggtggccc tggagccccg tgtcttcggc
     7921 tggatggacc gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac
     7981 aacggcgggg catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg
     8041 ttcaatgcca tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc
     8101 tgtctgatga cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat
     8161 tattaccggt tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc
     8221 atcgactgaa tggagagaag tacaatgcaa cagctttcct ttctgcccgg agagatgacg
     8281 cccggcgagc gcagcctcat tcaacgggcc ctgaaaaccc tggaccgcca tcttcatgaa
     8341 cccggcgtgg ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg
     8401 ggactggagc gtgaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc
     8461 ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt
     8521 aaacgcgccc tgtatcacaa tgccgctgct gtggtgctgg cacacaatca cccgtccggt
     8581 gaagtcacac ccagcaaggc agaccgcctt atcacggaac gtctggtaca ggcactgggc
     8641 ctggtggata tccgggtgcc ggaccatctg atagtcggtg gcagccaggt tttctccttt
     8701 gccgaatatg gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct
     8761 tctgtcttca gaggtatccc actatgaaaa tcatcacccg tggcgaagcc atgcgtattc
     8821 accgtcagca tccggcatcc cgtctttttc cgttctgtac cggcaaatac cgctggcacg
     8881 gcagtgctga agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt
     8941 ttgcagaacg ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga
     9001 actgatgttc cgtcacccag aaaacgagta cgactggctg agctcagacg ccgctgtgga
     9061 tgaggtcctg ctcatcaacg aatacacctt taccgaagcc ggtctgcgcg ccggctgacc
     9121 cgtcatcgtt aaactatcat tcaccgattt tctgtgagga ttttatcgtg tcagacacac
     9181 tctccgggac aacgcatccc gatgacaatc acgaccatcc ctggtgggga ctaccctgca
     9241 cagtgacgcc ctgtttcggc gcccgtctgg tgcaggaggg taaccggttg cattaccttg
     9301 ccgaccgtgc cggtatcaga ggccggttca gagatgcgga tgcgtaccct ctggaccagg
     9361 cctttccgct gctgatgaaa caactgaaat taatgctcac cagtggtgaa ctgaatcccc
     9421 gccatcagca taccgtgaca ctgtatgcga aggggctgac ctgtgaagcc gacaccctcg
     9481 gctcctgtgg ttacgtttat ctggctgttt atccgacacc tgaaacgaaa aagtaaacca
     9541 ccagaataac cttctgctcc ggcctggtgt tttcaccacg ccactgtccc attttttatc
     9601 tctgcatatc aggaaaatct tcagtatgaa aacattaccc gacacgcatg tacgggagtt
     9661 atcgcgctgc ccatctcccg tcaccatctg gcagacactg ctcacccgac tgctggacca
     9721 gcactacggc cttacgctga atgacacacc gttcgtggat gaacgtgtga ttgagcagca
     9781 tattgaggca ggcatttcac tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct
     9841 ggtacgtacc gaccagtcgg gattcagcgc ctgtacccac tctcagttaa taaacagcat
     9901 cgatatcctc cgggctcgcc gggcaacagg cctgatgacc cgcgacaatt acagaacggt
     9961 aaataacatt accctgggga agcatccgga ggcgaaacga tgaaactggc cctgacgctg
    10021 gaagccgaca gcgttaacgt gcaggcgctg aacatggggc gcattgtcgt tgacgtcgat
    10081 ggtgttaatc tcgctgaact gattaacaag gtcgctgaaa acggtgattc actccgcgtg
    10141 gtggatgaac gtgactctac cgaaacaccg gcaacttatg ccagccctca ccagttgctg
    10201 taagacaatg cagtgataca gggtatagtg ccattaacca cagaaacagc actgttgtcc
    10261 tgtattaagc aggcaagaga agatgtcctt accctgcgcc atctgcagct tctgcaccag
    10321 aaccagtgaa tctcactcaa caggcttcat ttcccctgac gccagcctga atacagctgg
    10381 cgttttcatt tataaacaga aaggaaaacc gataataatg gaatggacgc ccctaccatg
    10441 aagcaacggt aggatattac tgataaacct tccagtggag gtgcgttatg gccgttttaa
    10501 cgattagcat tgacctgaca aaaaatgaat tccaaatcca cggtctgggt aggaacagaa
    10561 aaatctaagc tcagaaaacg gattaaacgt tccctgatgc ccgtattctt cgcgaatatg
    10621 tcttcctgtc ggattgggat ggaggtctgc gccggagcgc gtcattagtg agaatgctgt
    10681 ctacttaggg gcataatgtc agactaatgc ccgcgatttg taaagccata tgtgaaaaca
    10741 aacaagaatg atatggctgc tgccgaggcc atctgtgaag ccgttacccg acctaatatg
    10801 cggtttgtca gcatcaagac gcctgagcag tagtcattac tgtcttttca tcgtgcccag
    10861 acggggtttg ttaaatcaag gactgcgcag ataaaccaga tcagaggatt actggctgaa
    10921 tttggcatcg ttctcccgcc cgacactgta gcgatcagtc gtcatgtccc tgccctgctt
    10981 gaagacgcgg aaaagtgttt aacaatgccg ttcagacagc tgtcgtccta tctctaatga
    11041 aaatgtgaaa caactgagcg tacacattga aaccatagaa gccgtgagca acgaacacgt
    11101 cgggaacgac gaactgtgca ggaaaattgc catcatttca gggattgcta ttcttaccac
    11161 cactgcaatt gtcagcaccg ggtagtatcg gcttcaaaaa ccggcgtcag cttgctgcat
    11221 ggcttggact ggttccccga cagtgcttca gcgtaggtaa aaacacgctg gggtctatca
    11281 gtaaacggga cgatgtctat atcagaacgc tactgttcca tggtgcgaag acagtgtcaa
    11341 acgccagaaa gggcagacag gtccaggaaa gctggctggg cagaccagtc tcgcggcgga
    11401 ataaaaatgt taccaccgta gcgctgaaca acaaaaatgc cagagtgctc tgggtattgc
    11461 taaccaacga taaagagatt tcaccagaag aagtgatggt agctgcatat atgtaaatga
    11521 aactacttct gtctgttgaa aaataaaacc aacctactga ttgctaagga taaacgagta
    11581 ttaaaggcca gactgataag accgcgacta agtgaacctg tatctgacat ggcatagctt
    11641 cgctaatgtg tcatcatgat taggactaaa tctgtgaatt cctttaggaa gcacggacac
    11701 aggccaatct gagtccatat gtatgattgg aatcttacac agatgacttt ttgacacggg
    11761 gacttggcca acaggagcga ccatgtatgt cagatattac aatctcactt ccgaaagaag
    11821 tcaacgggca cgttatctgt tcaaccagaa tccgacacat tctggctata cgaaagagca
    11881 tacttatgca aatctatatg cttttccggc aacaagcagg aatctcagcg ctgaggcagg
    11941 aattagaggt aaaaacgccc taccgggcca tgaatcaccc ggtcactgga gtagtaacca
    12001 aagcagattt agccagcatg gaacacatat ctttggtgaa atgttggtta cgggaagctg
    12061 gagcgcacaa cgtgttagta accagtgcgg ttaataacaa tggagttaca gaactttttg
    12121 ccttgcagca tacagaagaa ggctgttgct aatttcgata ggttctcaga tgagaatgat
    12181 gacggtgata cacagataag aatcaatgag caatgagggt tgccgggcaa ccctcattga
    12241 ataaaacagg aattacttcg cttcgccgtt tttcatttcg cccatagctt tcagcttttt
    12301 ggagatatcg cggcgttctt tggagagctc ggcatttttg atgatgtaat cgtcaacgcg
    12361 atcttcatag tcacctttca tgctggcgat gatgccctgg attgcttcaa cgctcatccc
    12421 tggcttgatg taatcgctca ggttgtccag caacaggacg cgcttctggt tgtcacggat
    12481 ctttttctca acgtcctgaa tttcacgttg cagtttgttc ttacgacgga acagacgaac
    12541 aaattccagt acgtcctgga atgaaggctt ggtagtttcc atttttatac ccctgataat
    12601 gtgagagtcg gattcgttta agcaaccgct attcgttagg gccaacatta ctcatagctg
    12661 ccgcagatga caatgctttt atcccttttc actatcatac cctttatcct tgctgaatcg
    12721 aagcagcagc aagatgattc tgaagttcag gaattatttg cgcaaggccc ggcaaatcag
    12781 attcgatagc aactcaagct gatgtgccgt ttccatgttc agccacacat aaccatagat
    12841 tggtgtttcc acgactttca ggtcatggtg gttatcgagc aactgacgca actcttccac
    12901 agcatcgttc aatttttccg tattgacaaa aaccggctgc ggattacctt cgtacagcgc
    12961 atgaacaagg ctcagcagta tttgctgcat catgtgctgg gtatcacgaa gtttttgcgc
    13021 attcaataac acgaaatggc tggggcgcgt ggcccagtat gcattgattt gcaactccag
    13081 catacaaacc agattgcgat taatggtctg gataccttca tatatcgatt tgggaatacg
    13141 ggtttctttg ctggcgggcg caatcagccc acgcattttc acggcatcgg tcagcagttt
    13201 ttgtagatgg ctttccagac gtgggcgttc gagtaagttc ggtgagaatg cagattgata
    13261 gacccgatta tactcggtca gacttttcgc cagttgaatg cgccagtgga tgaacgcccg
    13321 ttgtggccag ataccggtaa acaacattgc cagtaaagag ccgaggatca catcgccgct
    13381 tcgccataac gccgtatcaa tttcacctgt cggggaaccc acaacaattg ccagcgtcac
    13441 cccaatcaat aatccttgat acggtttctt gcccagcgcc agccaaccgc aaagaaacat
    13501 cgccgccgcg caccagacta acatcagcgg taacgaaatt aactccagtt gcagagcgat
    13561 aagacctaaa atcgacccca acaccgtacc gccaatacgt tcgaaggcgc gagggacaac
    13621 gttcccccag aacgagattg gccccataat caccaccatg gtgaccagcg gccaggtgct
    13681 ttccgggata gtaaacaggc ggataatgag aaaagtgagc aggaatgcca gcgcgacccg
    13741 agtaccatgc acaatgcggt aatggcggta tacccggatt tcaaacgggc ttaatgactt
    13801 atcggcacgc acgcagaaac tccgtaatga agattaaaag cgctcataac acatctgtcg
    13861 gccagaaggc gcgcctctga gttatccgac gaacataccg ggaacagtgt aatttcccgg
    13921 caggagcgaa aaatttgaaa ggcgcatctt actcttttcg ctttcaaaaa aagagtggtc
    13981 ttcgcgttaa cacaccgccc tgagatgaat tagtgatgtt ttggctgcac cgcaacatac
    14041 atttcgatat cccagtcccc atcttccgcg ccattgttca agtaaacctc gaagcagggc
    14101 tttggtaaca tttcataagc actgtcctgc aaaaggctat taaagaactg ataccagggt
    14161 ttagcaaaat catcaccgac tacacgagcc accgccaccg catactgacc acctgaaatt
    14221 tctgtcagaa tgacgccctc actgttttcg gggagcgtaa agttattcgg taccgtcacg
    14281 acggtgtcgc agcgtaattt ttcggcgggt gtttcatctg gattgtcgta atagacagca
    14341 acccactcct tcggcacaat atttttgcta tctacccaca tcatcaactg ctcaaaacct
    14401 ttttgtaccg tctgctccca cgggccaacg agatggaaac ctgcaacggt acgtttatct
    14461 tcctgcttaa tctcgtagtt catgacgcct ccattaacac tgtttttata tacagtataa
    14521 ttgcaatatt aaaaccacaa ggaatgagtg ttgattatgc gagcagactc gcactcctgc
    14581 cagtctgctg caaaagaaag gtcaggcctt atggtggaaa taatcactca ggcgggaaaa
    14641 catgctgcct tccccgacag attccagggt aaccagcggc cagtgcgcca cttgtttatc
    14701 acggtcgtaa agttcaattt cccctacccg ctgatgggcg ctaattggcg cggtgagttc
    14761 tttaccatca agggtatatt tagctttgat atgtggaatt tcggctttcg gtagcaccat
    14821 ccagaactct tgttccgttc ccagggcgat attttcttta tcgccatacc agatgcgttc
    14881 cgtaccaacc tttttcccac ggtgcaaaat ttgcaccgta gtaaagtttt gttgacccca
    14941 acgcagtaat tttcttgcct cttcctcacg accttttgcg ctgtcagcac ccattatcac
    15001 tgcaatgaga cgacgctgcc catctacagc cgaagcaatg aggttaaaac cggcaccaga
    15061 agtatggccc gttttcaggc cgtcaacatt catggtctta tcccacaata acccgttacg
    15121 gttttgctgg gtgataccgt tccaggtgag gcttttctca ctgtacatat gataaaactc
    15181 gggctcgccg tggatgatag cgcgagaaag cacggctaaa tcataagccg agctatgctg
    15241 gccaagtgca tccagaccat gcactgtttc aaaatgcgta tccttgagat gcagcttctc
    15301 ggcatagttg ttcatcattt caacaaactg ccgttgccca ccggcaatat agtcagccag
    15361 agcaacacaa gcgtcatttc cggaatccac aattaaacca cggcttaaat cacgtaccga
    15421 tacgcgatcg ccctctttca aaaacatcag tgaagaaccg acaaacaccg gattatcttt
    15481 cgcccacgca tcacgcccca cggtgacaat atcgtctggc gtaatgcgat gactatcgat
    15541 agcgcgatcc acgacataac ccgtcatcag ctttgtcagg ctggcgggat tgcgctgttg
    15601 atgctcatta cccgcggtga gaatctgacc ggtggtgtaa tccatcagta cccaggaccc
    15661 ggcttgaatc tgtggaggct gaggtgaaaa aggaatgttt tccgccgcaa aaccagacga
    15721 taagttaaaa acgaacaaag aagcagcaat aataagacgg cgtttcaaca gcaaaccctc
    15781 aggagtttca aatagctgtt ctttttacgg aaatacttat gaactggctg gaataaagtg
    15841 caagaaaatg tgactaccct cccattttta tctgacatga tctgttgcca ctcgctgtca
    15901 aattatcgcg ctaaagctga ttagcacggt gatatttgat actctggcag acagcagaaa
    15961 taacggattt aacctaatga tgaatgacgg taagcaacaa tctacctttt tgtttcacga
    16021 ttacgaaacc tttggcacgc accccgcgtt agatcgccct gcacagttcg cagccattcg
    16081 caccgatagc gaattcaatg tcatcggcga acccgaagtc ttttactgca agcccgcgga
    16141 tgactattta ccccagcctg gagcagtatt aattaccggt attaccccgc aggaagctcg
    16201 ggcgaaagga gaaaacgaag ccgcatttgc cgcccgtatt cactcgcttt ttaccgtacc
    16261 gaagacctgt attctgggct acaacaatgt gcgtttcgac gatgaagtca cacgcaacgt
    16321 tttttatcgt aatttctacg atccttacgc ctggagctgg cagcatgata actcgcgctg
    16381 ggatttactg gatgttatgc gtgcctgtta tgccctgcgc ccggaaggaa taaactggcc
    16441 tgaaaatgat gacggtctac cgagctttcg ccttgagcat ttaaccaaag cgaatggtat
    16501 tgaacatagc aacgcccacg atgcgatggc tgatgtgtac gccactattg cgatggcaaa
    16561 gctggtaaaa acgcgtcagc cacgcctgtt tgattatctc tttacccatc gcaataaaca
    16621 caaactgatg gcgttgattg atgttccgca gatgaaaccc ctggttcacg tttccggaat
    16681 gtttggggca tggcgcggca ataccagctg ggtagcaccg ctggcgtggc atcctgaaaa
    16741 tcgcaatgcc gtaattatgg tggatttggc aggagacatt tcgccattac tggaactgga
    16801 tagcgacaca ttgcgcgagc gtctatatac cgcaaaagcc gatcttggcg ataacgccgc
    16861 cgttccggtt aaactggtgc atatcaataa atgtccggtg ctggcccagg cgaatacgct
    16921 acgcccggaa gatgccaacc gactgggaat taatcgtcag cattgcctcg ataacctgaa
    16981 aattctgcgt gaaaatccgc aagtgcgtga agaagtggtg gcgatattcg cggaagccga
    17041 accgtttacg ccttcagata acgtggatgc acagctttat aatggctttt tcagtgacgc
    17101 cgatcgtgca gcaatgaaaa ttgtgcttga aaccgagccg cgtaatttac cggcactgga
    17161 tatcactttt gttgataaac ggattgaaaa gctgttgttc aattatcggg cacgcaactt
    17221 cccggggacg ctggattatg ccgagcagca acgctggctg gagcaccgcc gccaggtctt
    17281 cacgccagag tttttacaag gctatgcaga agagattcag atgctggcgc agcagtatgc
    17341 cgacgataaa gaaaaagtgg cgctgttaaa agcgctttgg cagtacgcgg aagagattgt
    17401 ctaatgtgaa gcctccgccg ctggtacggc ggaggacaat gttgagtcag gctttttgaa
    17461 cggtgatgct ccacgctgca tcgccaatct gctgataatc ggtgatggca tgtccttctt
    17521 ctgccgccca ctgtgggatt gcttcagttg cctgggtgca atcaaactca attaccaact
    17581 catcgccgct ggccatttct gccagtgctg cttttgcttc tatgagtgga aacggacaga
    17641 cctgcgtcac cacatccagc tttttaatcg ccatctttac accttactta atttgccgca
    17701 gcagttgcca gtcgcgcctt acgctgcggt cgaacataca caagccagga cgcagtccag
    17761 actccgagaa tcataaatac aaggccaatc cagccctgcc aggtcatcat tgcagtcata
    17821 accagtccat taccgataga gcaaccacct gcaatactgg caccgaagcc catcagtaca
    17881 ccacctaacc cgctacgtaa tgttgtttgt gcatcagctg cgcgaacgcg gaactcacgg
    17941 ctcgctttgg ctgcaataaa tgaccccacg aagatcccta acaccagaaa aacgccccag
    18001 ttaatgtatt tcacgtcacc cgcgaccaga aattgcagaa tattggccgt tggggaagtg
    18061 attcctaacc caaacatgcg tccggttgct tcactcaggg gccaggctaa aagcgcaatc
    18121 aaaccgatga gtacagcagt gacaaaggga tgccagcgtt tttcaaacag aatatgagcg
    18181 atcccggttc ggcgcggcgg taaggtcgcg actttgagtt ttggcttctt caactctttc
    18241 atcaccaccc agagcgttat caccagcaaa acggtaacca acggccacac agacaaatta
    18301 aaagtctcag caatagagtt atgttcagta ctgtagtact gcaaggtttg atttaaacca
    18361 ctggcgtgtg gagaacgcat caccgcactc atcaccatat aagtgaaaag cgcgatccaa
    18421 ctgccgatca atccctcacc cgcgcgatac caggtcccgg tggcacatcc acccgccaga
    18481 acaattccca gcccgaaaag atacccacct ataacagtac caagccacgg gaacgccccg
    18541 gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa cccccacgct ttgtaccgaa
    18601 attgcaatca gcaaggcgta aaacatgcga ttatttttcg cgatatacat atcgcgaaaa
    18661 ccacctgtca ggcaaaaacg cccacgctgc atgacaaatc ccagcagagc accacaaatt
    18721 agcccgctta atatcattga aaacataaat taattaacca tatgaatgtt aatggggaaa
    18781 ttattcatga ctggtggaat gcagaccaat aaccaaattc tctaaattag aacaaatggt
    18841 tattaatgag gatttcgata aaaaagaata aaaaaaccgg agccaaatgt tcattcgact
    18901 ccggtttatt attagaaaag atgattatgc tacgtcttcg tactgcggaa ccggattacg
    18961 gaaactcttg gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag
    19021 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
    19081 cggcaggaac agatactgga agtgatcttt cagcgtctta ttacgcttct cacggatcca
    19141 gaactgcgag atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat
    19201 cagcgccgta gccattacca ggtcgaagtt gattgccagc aaggcaatcg cgccaaccag
    19261 gatgatgttc atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
    19321 gaacacgccg tcacgaccca ttacgtacat cagacgcgcc acgcctgcgt gcgccgccat
    19381 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
    19441 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc
    19501 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc cgatcagcgc
    19561 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
    19621 gctgatgccg tcaaaaccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg
    19681 gataacatgc gcatcaccag accagaacgg acgagtgctc gccagcgtac cagcgccttc
    19741 accttcaaat acaccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa
    19801 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
    19861 cataaaggcc accagcgcca ccacaaacat ccacgatgga atggaaggca ccagagcttc
    19921 aaaatagatt ttcgccaaca gaatgttgat catcggcgcg aacagatagt cgagcagaga
    19981 agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc
    20041 agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat
    20101 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
    20161 accaaatgta tcaaacagcg tcatcggctg catataggct agacccatca ttacaaccgg
    20221 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
    20281 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
    20341 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
    20401 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctaa ttaaacgtcg
    20461 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
    20521 aatgagaggc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
    20581 gcataaacac cggcgatcgc actatttgct aaaatttcat ctcgccacca gccgctggca
    20641 agccctgcgg tctgttcatt ccagccaatc cagacgggtt caaagagagt ttgggcgacc
    20701 actttcttct cgattaacgc gccactatcg agaaaacgtt gcgccagata ccgtggtaaa
    20761 tagccgcagc caaggccgct aatttgcagc tccagtttgg ttttaaaatc gaaaacggta
    20821 atggcttctt gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtgtcc
    20881 cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttcctgg
    20941 gctaacggat gatgaggcgc gaccgcaaag acttgttcaa gatcgcccaa ccgcgagaaa
    21001 ccaaactcac tggaagatgg tggctcatgc atcgcgccaa cgataatatc cgctcgcccc
    21061 tgagtcaggg catcccagga accaccgagt acaccattga taaacttcag gcgcgtcacg
    21121 ctatgatgtt gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg
    21181 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
    21241 agttctcgca cggtatgcag aacttcccgc cccttctcta ataacatttt tccggtacgg
    21301 gtgaatttag cgcggtgacc gctacgatca agcaattgaa tattaaggtc gctttccagc
    21361 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga cgccgccgca
    21421 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata
    21481 cacgccccct tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
    21541 accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca
    21601 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccagt
    21661 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
    21721 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
    21781 aaatggacta aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt
    21841 ccgggatgac gtcccggtcc gaccagtccc gcaagacgca ggatatcgac cgaagtacca
    21901 ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt ggttaccgga
    21961 ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgttgagctg
    22021 gtaaaaataa tgcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct
    22081 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
    22141 tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
    22201 agcagatagc tatcaatgcc actcattcgg gctgcttcga caccatcttg tgtggtttta
    22261 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
    22321 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata
    22381 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg
    22441 ataacgacat gctgcaatga gcagtgaatc gtcggtaacc gcgtatacca gacggtgctc
    22501 ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggcttccc
    22561 cttaccttca aatggcgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat
    22621 tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag accagattag
    22681 tttcactcaa taatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc
    22741 atcaatctcc gggcgttagc gggggaacgc agtagataag ccgtctcttc cagcgagttg
    22801 tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat gaggatcggg
    22861 gcatgatctt c
//
LOCUS       DACAPB010000059        22775 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.059, whole genome shotgun sequence.
ACCESSION   DACAPB010000059 DACAPB010000000
VERSION     DACAPB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.059"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(127..1530)
                     /gene="eutA"
                     /locus_tag="IT904_002073"
     CDS             complement(127..1530)
                     /gene="eutA"
                     /locus_tag="IT904_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAP3191854.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(1527..2753)
                     /gene="eutH"
                     /locus_tag="IT904_002074"
     CDS             complement(1527..2753)
                     /gene="eutH"
                     /locus_tag="IT904_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAP3191855.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(2970..4157)
                     /gene="eutG"
                     /locus_tag="IT904_002075"
     CDS             complement(2970..4157)
                     /gene="eutG"
                     /locus_tag="IT904_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAP3191856.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(4147..4983)
                     /gene="eutJ"
                     /locus_tag="IT904_002076"
     CDS             complement(4147..4983)
                     /gene="eutJ"
                     /locus_tag="IT904_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAP3191857.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCLQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(4994..6397)
                     /locus_tag="IT904_002077"
     CDS             complement(4994..6397)
                     /locus_tag="IT904_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAP3191858.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLHRCVLVDAFRIV"
     gene            complement(6409..6696)
                     /gene="eutN"
                     /locus_tag="IT904_002078"
     CDS             complement(6409..6696)
                     /gene="eutN"
                     /locus_tag="IT904_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAP3191859.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(6803..7096)
                     /gene="eutM"
                     /locus_tag="IT904_002079"
     CDS             complement(6803..7096)
                     /gene="eutM"
                     /locus_tag="IT904_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAP3191860.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(7135..8151)
                     /gene="pta"
                     /locus_tag="IT904_002080"
     CDS             complement(7135..8151)
                     /gene="pta"
                     /locus_tag="IT904_002080"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP3191861.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(8148..8951)
                     /gene="eutT"
                     /locus_tag="IT904_002081"
     CDS             complement(8148..8951)
                     /gene="eutT"
                     /locus_tag="IT904_002081"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAP3191862.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(8948..9649)
                     /gene="eutQ"
                     /locus_tag="IT904_002082"
     CDS             complement(8948..9649)
                     /gene="eutQ"
                     /locus_tag="IT904_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAP3191863.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(9624..10103)
                     /gene="eutP"
                     /locus_tag="IT904_002083"
     CDS             complement(9624..10103)
                     /gene="eutP"
                     /locus_tag="IT904_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAP3191864.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFNHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(10116..10451)
                     /gene="eutS"
                     /locus_tag="IT904_002084"
     CDS             complement(10116..10451)
                     /gene="eutS"
                     /locus_tag="IT904_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAP3191865.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(10743..13022)
                     /gene="maeB"
                     /locus_tag="IT904_002085"
     CDS             complement(10743..13022)
                     /gene="maeB"
                     /locus_tag="IT904_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAP3191866.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            13311..14261
                     /locus_tag="IT904_002086"
     CDS             13311..14261
                     /locus_tag="IT904_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase A"
                     /protein_id="HAP3191867.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            14281..16284
                     /gene="tkt"
                     /locus_tag="IT904_002087"
     CDS             14281..16284
                     /gene="tkt"
                     /locus_tag="IT904_002087"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP3191868.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIEHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(16379..17422)
                     /locus_tag="IT904_002088"
     CDS             complement(16379..17422)
                     /locus_tag="IT904_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAP3191869.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(17548..18123)
                     /gene="nudK"
                     /locus_tag="IT904_002089"
     CDS             complement(17548..18123)
                     /gene="nudK"
                     /locus_tag="IT904_002089"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAP3191870.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(18191..20170)
                     /gene="aegA"
                     /locus_tag="IT904_002090"
     CDS             complement(18191..20170)
                     /gene="aegA"
                     /locus_tag="IT904_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAP3191871.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            20376..22076
                     /gene="narQ"
                     /locus_tag="IT904_002091"
     CDS             20376..22076
                     /gene="narQ"
                     /locus_tag="IT904_002091"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAP3191872.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            22240..>22775
                     /locus_tag="IT904_002092"
     CDS             22240..>22775
                     /locus_tag="IT904_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP3191873.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYG"
BASE COUNT         5214 a         6472 c         5917 g         5172 t
ORIGIN      
        1 caaaacattt ttcaccacaa aagcatccaa tcatcttgcc agaccaatca ccttaaccgg
       61 atacactgcc ggatataatc ccactaacac ccagccacta agcaccatac ctgataacta
      121 ccgtattcag gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg atccgccaaa
      181 aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt caatgactgc
      241 caacgggagt tgttgtagct gtgggcgcaa caacatgccc agagctttac caaagtcctg
      301 cccggccacc accagcaggg gatgcggatt cggaaaacgc gcgacgaaat cgaccagcgc
      361 gttgatgacc gtcagtaccg cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac
      421 gtacgcgtca gttttgggat caagatccag ctgaagcagc gcctgttgcc aggcactcac
      481 cagatccgtt tcatcaatcg ggatcgccac cggcaaattg cgcagcggca gttgtacgcc
      541 ctccagccag attgtgctgc cagagagcga aagggtatgt gcacccgcgc caatcaccgt
      601 ggcgcgtacg gtttgcgccg gaaactgcac attcatctca cgcaggcgcg gatggtcatg
      661 cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag aacgggtcgg cgggctggtg
      721 gcgataacat tcgcccacgc ctccagaaag cgtaatgatt tcgggcgtaa cacctgcggg
      781 cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa agcgttccgt caatcacttc
      841 gacaatcagc gccgccatcc gccgggtaac ctgcaccagc tgcgcgccgg tcagcgaacg
      901 ggcgtcagtg cctgcaccga agcactcatc cacaatcatc tgccccggtt tatgagcgta
      961 aaccacgcgc ccctggctgt cggtttccag caggcgacca ccgacgttga gacaggcagt
     1021 gccgctgatt tttccggcat cgaacagggc gtagttcgcg gtgccaccgc cgatgtcgat
     1081 attcagtacc cgacacagcc gttgttcaga aagggtttgc gccccggctc cgtgaccggc
     1141 gatcacggat tcgaggtgcg gcccggcgct ggcgacgaca aaatcgccca gcgactgaga
     1201 gagcgccatc accgccgggc gagcattgcg ggttttcgcg ctttcaccgg tgatgatgat
     1261 ggcaccagaa tcaacgcttt ccggcgcaat gcccgcagcc tgatattgct ctaaaatcag
     1321 cgatttcagt tccgcttctt ttaaaccacc ctgtttatcg acaggggtaa agaacaccgg
     1381 gctttgccag ctaatttcgc gtttgatgaa ttcgtagcgc ggcacctgcg acaccgccgc
     1441 acggttaacc agctccaggt gggagaaaat cacctgggtg gtggtggtgc cgatatcgat
     1501 accgacgctc aatagctggc gagtgttcac gattgtgcct ccgcttcggt tttagtcgcg
     1561 gtcgcgtctt cttttggcac cagcatcatc gccacgccaa tcgccgttac gccgccgatc
     1621 aacttgccga caatcatcgg gaagatcatg gcgttcatgt tggcagcggc gaagcctaag
     1681 tggtcgccca gggcgaaagc agcggaaacg gcgaaggcgc agttgatgac tttgccgcgg
     1741 gtatccatct gcttcatcat gccgaacatc gggatgttgt tggcaagcgt tgccaccatg
     1801 ccggctgccg cgatgttgtt catattcagt actttaccga cgctcatcag cggtttttca
     1861 aaccagcgag tcagcagcag caccatcgga tacgccccta acagaacgca ggagatagaa
     1921 ccgataactt caatggcgcg catcacctca ccgggtttat cgccaggggc cataaagata
     1981 ggatccagac cggggatcag ttcccagcca agcaggaatt tcactaccgc agcggcaaga
     2041 ccgagggtga tcaatgcaac gaggaatttg gcgaagatct ggaagccgtt gatcattttt
     2101 tccgggatga atttcagccc cagcgccacc agaatcgcaa caatgatcac cgggatcatg
     2161 ttcatcagga tcagggcgaa agtgaattcc accggctggc cgttgatctg cacaccggag
     2221 tacatagcaa ccagaccacc agcgatacaa ccaatcggaa tggtcacaat gcccgctagc
     2281 acgccgagcg ccagataacg acggtcagaa ggttcgataa tgccgagcgc caccggaatg
     2341 gaaaacacaa tcgttggccc catcatcgac ccgagaatta acccagagta tagccacgcg
     2401 gctacgtcgc cgcccgccag ctctttggcg aggaagaagc cgcccatatc gcacgccagc
     2461 agtgttccgg cgaacatcga tgggttagcg ccgagcattt cgtaaaccgg aataattacc
     2521 ggcccgagaa cgtgagccag taccggtgcc agcgcggtca taccgaccat cgccaggccc
     2581 agtgcgccca ttgccataaa gccttcttcg aactgaccgc ctgatccttc gatactttta
     2641 ccgaacttac cgaggaaacg agcagaaccg ccgaactgcg acaggatcct gtctacggca
     2701 gctatcagca taaagaacat catgatgtac atgatgattt cgttaattcc catcgccttt
     2761 actccctgtt agttgttatt tattggcgga tgcggcgtaa acgccttatc cgccctacat
     2821 gtgcaatccc gtaggctgga taagacgcgg caagcgtcgc atccggcaat tgcaccgcgc
     2881 cactggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc gtaggctgga
     2941 taagatgcgg caagcgtcgc atccggcatt tattgcgccg ctgcgtacag gccgacaatc
     3001 tgctccaggc tggcggtacg cgggttactg cgcagacaaa tatcttccag cgcggcctgc
     3061 gcccatgcgc cgtaatgcgc agatgtcgca ccaacatcgc ccagtcgttt accaatccca
     3121 acttccgcaa tcagctcact taccgcgtta atagcgtcac gatcgtcgga ttttttagtt
     3181 cgcagtgccc gaccaatctg actaaagcgt tcacgacaaa ccatccggtt aaattccatc
     3241 accgttggca gcaacatggc gttcgcgaga ccgtgcggaa tatgcagcgc cgcgcccggc
     3301 tgatgcgcca tcgcgtggca caacccaaga cccgcactgg aaaacgccat tcccgccata
     3361 catgaagcca gtaacatgct ctcgcgcgcg gcaaggtcgt gaccgtagcc caccgctttc
     3421 ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca ggctgtcggt aaacggtgta
     3481 gcgttcaggg cgctgtatgc ttcaatggca tgggttaacg catcaatgcc ggtcatcgcc
     3541 gtgacatgcg acggcacacc ttcggtcaat gcggcgtcga ggatcgccac atccggcatc
     3601 agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg cgtcgataat caccgttaca
     3661 ttggtggttt cagagccggt tccggcggtc gttggaatgg caatcagcgg caagcgcggt
     3721 tgcagaacgc tggtttctga catctctgcc agcgtgctat cggggttcgt caccagcaac
     3781 gccacggctt tcgccgcatc cagcaccgag ccgccgccaa atgcgatcac cccatcacag
     3841 cctgactcac gcaactgcgc cacggctgca cacacgtcgg taatgcacgg ttcgcccacc
     3901 ggacatggcc agagcgtcat ggcgatgcct ttaacagcca ggctgcgcgt cagcccggcg
     3961 gtcatccccg cctgatgcaa aaagctgtct gccatcacga acagatgttt cagcccacgc
     4021 gtttgcgctt gctgcccgca actgctcacc gcgcccggac cgcaaagcgt caccggtgga
     4081 acgctaaatg tttttacccg ttgcagattc agggtatcga acgcctgaaa gagcgcggtc
     4141 tgcaattcat tttgcatata gcccctccgc tttctctcgc ccactactgg cgatcgccag
     4201 cggggtcata aacaaactgt gctgcggtaa atgcacctgt aacgccggga attgtttgcg
     4261 aaacagttcc gccacgcccg gttgcaaaca agagccgccc gccagccata aatcagtaat
     4321 gccctgccct tcaatatgcc gggcgacaat gtccgccatt ttttcgtaca ccggtttcac
     4381 cgcaggccaa atctcttcac cgtgaccgcg cttgtactgc tccgcctctt ccagcgaaat
     4441 acggcgattt ccggcgagag tcagagagat gtgatgtccg ccggtggctt catccgccga
     4501 gtacgtcacc ttgccctttt tcacgatggc aatgccggta gtgccgccgc cgatatccac
     4561 tacaccggcg ttgtccagtt gcagcagatc cgcaactgcg gttggctcat ccagcacatg
     4621 gctcacttcc agcccggcag attccagcac gttgatggaa atacgcgggt ccgtacctgg
     4681 aggaaatgaa gtcgccgcat ggctaaaacg aagaccgaat tgctgctcaa gagtgtcgag
     4741 atggcgacga acaatggtga ccgcaccgaa gaaatcccag acgatgccgt cgcggaccac
     4801 atcggcccag tcgaggcata ccgccaccgg ctgaccgtcg cggtcaacaa ccatcgacac
     4861 cacatcacag gtgcccagat ccacccccag ccacagcgga gattccgtcg cggcgggcgt
     4921 ctggttacac agcgttgccg ccgtttgcag acgtggggtg agccattgtt cgtcgtgcgc
     4981 catctgttac tccttaaaca atgcgaaacg catcgactaa tacacagcga tgcagacgga
     5041 caaacgtacg cgcgctggtt accccttcac cggttggcgt ggtgatggtc atggtggtcc
     5101 agccttcccc gcccagcccc agcccggcaa tgcacggtcc gttcttaacg aaaatgctgg
     5161 tatcaatagc attcgccatc tggttcatgt tttcgatgtt gcgcgagtgc attgccgccg
     5221 tgtggtggca accgccttcc agtttcaccg ccagcgcaat ggcatccgcc acgttggcga
     5281 cgcgcacgac gggcagcacc ggcatcatca gttcggtcac ggcaaacgga tgttctgcag
     5341 tggtttccac aaacagcaaa cgcgtttctt gcggcacgtt caggccgatt gccgccgcga
     5401 ttttcgccgc atcgcgacca acccagtcac ggctgacggt gccttttccg cgctcgtcga
     5461 tatttttcag caacaccggt tgcagctgct gcgcctgttc tgcggtcagt ttcaccgcgt
     5521 gctggccttc catcagacgc atcagttcat cggctacgct atcaacaaca atcagtacct
     5581 tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc tttgacgatg gactgagcgg
     5641 cacgggcgag gtcggcggtt tcatccacca ctaccggcgg gttgccagcg cctgcggcaa
     5701 tcagacgttt attggtgtgt ttacgcgccg cttctactac cgcttcgccg ccggtcacca
     5761 ccagaaggcc gatgcccgga aacttgaaca aacgctgcgc ggtttcgata tccggatttg
     5821 ccacagtaac cagtaagttt tccggcccgc ctgcggcaac aatcgcctgg ttgagcagcg
     5881 tgatcgcccg ctgggagact tttttcgccg ccggatgcgg ggcaaaaatg acgctgttgc
     5941 ccgcggcaat caggctgatg gcgttattaa ttacggttgc cgccgggtta gtggacggag
     6001 ttaccgaagc caccacgccc cagggcgcgt tttcaattaa agtcaggccg ttgtcgccgg
     6061 tcagcacctg cggagagaga cactcaacgc ctggcgtgcc gcgcgcctga gcgacgtttt
     6121 tggcaaattt atcttcaacg cggcccatgc cggtttcact gacggcaagt tccgctaaat
     6181 ctctggcgtg tttttcgcct gcctcacgaa tggcagcaat ggctaactgg cgcattgcca
     6241 cgctttttaa cccttgctgg gcgactttgg ctgccgcaac ggcgtcatcc agggacgcga
     6301 aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact gctttgcatt ttcagcagta
     6361 ccgctttcac cacctgttca atatcctgtt gattcatgat gttctgcctt atttgtggaa
     6421 aattacctga ccgccagaca ccacctcatc gacaatgcca atcacgcaca gatcgaccgg
     6481 tgacgtttcg cttttatgcg cctggcgggc ggaactgcca ctcaccagta acacccactc
     6541 cccggttccc gcgccaatat tgtcgatggc gacggcgcat tgcccgtcag gattaccttg
     6601 tggatcaatc atttccacca tcagcaattt gtcatgcgcc agtccgtgat ggcgtacggt
     6661 acaaacaatt tgtccagtga cgactgccag tttcataccc gcctccgtgg cgtatttcag
     6721 gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa aaggagagag cgtgacgccc
     6781 gaatcgacgt cacacagggt gattacaggt tgctgctatc gcctttcagg ccgatcggga
     6841 agacttcttc caggtcgccg tgcggacgcg ggataacgtg tacagatacc agctcgccga
     6901 tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt acaggctgca acatcaccac
     6961 gaaccatggc agtacacagg ccgccgccaa tctgcttaac accaaccagc ttgacgcgcg
     7021 cagctttcac catcgcgtca gaagcctcaa tcagtgcaac caggccccgg gtttcgatca
     7081 ttcctaatgc ttccattgtg ctttcctctt tatcagggtc cagaacggga ccgttcattc
     7141 aaccagtgtt tgtaaactgc tttcacggtt cacttctgtc tgacgcggca cagctgccac
     7201 cagcgccagc tcgataattt cctgcacgct acaaccacga gagagatcgt gcatcggcgc
     7261 ggcaagtcct tgtatcagtg gcccgacggc acgatatccg ccgagtcgtt gtgcgatttt
     7321 gtaaccaata tttccggctt ccagcgacgg aaaaaccatc acattggcct tgccctgtag
     7381 cgggctggca ggcgcttttt gcgccgccac ttccggcacg aaggcggcgt caaactgtaa
     7441 ctcgccatcc accaccagct ttggtgcgcg ctcacggacg atttctgtcg cctgctggac
     7501 gttagcaaca caggggtgac gggcgctacc attgctggaa aacgacagca tcgccacgcg
     7561 cggctcttct ccggtgatgg cgcgccaggt ttcggcactg gcaagcgcga tatccgccag
     7621 ctgcgccgcc gtcggctgtg gcaccacgct gcaatcggca aagcccaacg ccgggccgct
     7681 gtactgtggc aacatcagga aaatggaaga gagcgtttta cagcctggct gcaagccgat
     7741 aatgcgtaat ccggcacgca gcacatttgc cgtggaagag aggttgcccg cgatacagac
     7801 atccgcttta ccggcgctga ccattgctgc ggcgaacatc agagggtcgg taagtttttc
     7861 cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc cagcgatgag caaattcttc
     7921 ccgcattgcg aggttgccat gcggatcaat cacctgtagc ccgtccatcg ccacgccgtg
     7981 actgagcgca aactgacgaa gttcaaacgg attggcgacc agaatgggcg ttgccagacc
     8041 ttgttgatgt aaatattgcg cggctttcag cacacgttgg tctaacgcat ccggaaaaac
     8101 cactctggcg ggcgctcgca acgccagttc acgacaacgt tcaataatca ttgcgtttcc
     8161 cccagtcgtt gttggatctg cttcaccgtt agcggctgct tcgttacgct caggatcatc
     8221 atcacgtaga ccgtgctcga aaggcggttc agcgcctgca aaatgtccgg gcgtaatact
     8281 tcaaagctgc gggtgataaa gacctgcgcc gctacggttt cggtttcccg cactttggtg
     8341 cgtagcagat tgagcaacgc agcatcgcgc ccgtggctgg cttccggcac cagatgatcg
     8401 tggtcgagat agcgcagcgg ctgatgagaa agccgatgca gatcttcgtc agaaagcccg
     8461 acaatcgcct ggcagcccag cggttccccc agcgcatcgg cgcgcataat gttgccaaga
     8521 cgcgaacgga tatccgccag ccacggctgc cacggttccg ccagttcaat ttgcagccac
     8581 actgccagcg caatggtgct gtcgagaacc gcgcgaaaac ccagacgcgg atcgcttttg
     8641 gcgaccattt tttccgccga caggtgagtc agcgtatccg gcttttttgc caccggctgg
     8701 cgacacagtt cgcagcacgc ctgcggatgt tcatcgctac tggtcagccc atgaacgggc
     8761 tgcggctgct gctgttcatc gtcaacaaac aggcggccct gctcgtcaat aaacttgatg
     8821 cgcagatggc ggctttccag caactcccgg gcagagggcg tcaggcgact gtccgcaggg
     8881 agatggatct ctgcgccttc gctgagcgta tggttcgctc ttagccatgc ttcggtgatg
     8941 aaatctttca tagggattgc cagttagccg gccaggcgac atacaggaat ttcacgctgg
     9001 atgtcgtacc aaattcgatg ctggagcctt tcgggataaa catcacgtcg cctgctttgg
     9061 cgatcatggt ttggccttcg tggcgaacat gcagctcccc ttccagcacc atgtcgattt
     9121 cgtcgtagtt cagggtccac gggaagaatg cgttctccca ctgcataaaa ccggcggcca
     9181 tgctgctgcc gtcgtctccg gtcacaagat cagtcaagcc cacgcaatgc ggctctgcgc
     9241 cgtcgaaacg accaaacttg acgctactgc catcaatcac tttgatgccg cctttgccgg
     9301 tcaccgattt gaagctcggc tgcattgcgc cctgctccag cgactgtttt tccttcatca
     9361 ctttttccat cagctgtgcg accaggcttt cggtaaactg gccttccggc agctgggcaa
     9421 tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc tggcacgctg gcgggcacgg
     9481 acgccgttac cgggatcgat tcatcacatt cggtgatggt aaaacccagt agatccgcca
     9541 cttcacgggc ttccggggta ataatgctgg cgcgcagaac cacggacatt gcctgttcgc
     9601 cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag ttttttcacc tgcttcctcc
     9661 tgtttggtga gagatgccag ataatccacc agttgctgca cgctttgcgg gtcgtggctg
     9721 tttagctcga aaatcggctc ttcgaaaccc gtctcaagca ataactttcg cgttgcggcg
     9781 acgtcagcat ctggcatgtc cgttttactg atgacggcga tttgccgttt gctgacgcca
     9841 atatccagca acccggcagg caaccgactt tccgggtcat ttgcgccatg gacatagatc
     9901 agcatgtcta catcctgtag cgtggtaatt aaggcgtgat accagcgggg atggttaaaa
     9961 tattcacccg gcgtatcaat atcgccctta tcattaaatt ccacggcctg tgtttttctg
    10021 gcgagggtat aattcccctg caacgcatta aataacgttg tttttcctgc cccgacgcta
    10081 cccacaaacg caattcgttt catgaatgcc tcgtattaac ttttggtcat ttcacaaagc
    10141 gtataattta ataagcgacc taaaccgctg acggtttgtg ataacgcttc ttctactgcg
    10201 cccaccgagc cataaatcac caatgcaccg ctgaatcggt cgagaaaacc gatatgcaca
    10261 tcagcggctt tcagcgctaa atcaccggcg atcatcgccg tttcgccggg agttagcgtc
    10321 ataatgccaa ttgcgcccgc atccggaacg ccgattttct tcgccagttc ctcaccggga
    10381 tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca caaattcctg aatgatgcgt
    10441 tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt atcgtgacaa tttttcgtca
    10501 gagtaaataa caaaattcgg caagcgcgtt taaaaggtga ggtagatcac taagaatgga
    10561 gagatattcg ctgtggtgca taaactttca tatgcaacgc atgaatataa ataaaataat
    10621 atcgagatat tatatagact gcttttttta ttattcgcaa actcagtaat aagctttact
    10681 aatatattgc agctaaactg cttaccctga atattcaggg taagcgtgag agttaaaaaa
    10741 aattacagcg gttgggtttg cgcttctacc acggccagcg ccaccatgtt gacgatacga
    10801 cgcaccgatg cgatcggcgt taacacgtga accggtttcg ccacacccat cagcaccggg
    10861 ccgacagtca caccttccga gctggaaaca cgcagtaagt tgtaactaat gcgggcagct
    10921 tccatgttcg gcatcaccag aatattggcg gaacctttca aagagctgtc cggcatacgg
    10981 tcgttgcgaa tcgcttccac cagcgctgca tcgccgtgca tttcaccatc aatcatcagt
    11041 tctggtgcac gttccctgac cagttccagc gcctgacgca ttttgctcga cgacgggcag
    11101 tcagaagaac caaagttgga gtgcgacaac aaagcaacgc gcggctcaat accaaaacga
    11161 cggacagttt ctgccgccat caaggtgatc tccgccagct cttctgcatc cggttcatca
    11221 ttaacatatg tatcggcaat aaaggtgtta ccactcggca gcagcagcgc gttcatggca
    11281 cctgcggtgt gaacgccatc gcgataacca aagacatttt tcaccacgct aaaatgttca
    11341 tgataatcac ccaccgtacc gcaaatcatt gcatcggctt ccccacgctg aaccatgatc
    11401 gcgccgatca ctgtcgggtt actgatcagc gcccgctgcg cctgttcctg agtgacgccg
    11461 cgacgcttca tgatctggaa gtattcggtc cagtactctt taaagcgcgg atcggattcg
    11521 ttattgacga tctcaaaatc aacgcccgct ttgatctgca agcccagttt ctgaatgcgc
    11581 atttcgatca cgttcggacg accgataagg atcggtttcg ccagtcccag cgttaccagt
    11641 tcctgagtgg catgcagaac gcgcgcctct tccccttccg gcagaacaac gcgcttcggc
    11701 gctttgcgag cctgggagaa aatcggcttc ataaacaggt tggttttgta aacgaactca
    11761 gtcagcttgt cgatgtagac gtcgaaatca gcaatcggac gagtcgccac gcccgactcc
    11821 atcgcggctt tagcgaccgc aggagcgatc ttaacgatca agcgtggatc aaacggtttt
    11881 ggaatgatgt attccggacc aaagctcaga tcctgatcgc catacgctga agccaccact
    11941 tcgctctgtt ccgcatgggc gagttctgca atcgcacgta ccgccgccag tttcatctct
    12001 tcgttgatgg cggttgcgcc aacgtccagc gcgccacgga agatgaacgg gaagcacagg
    12061 acgttgttca cctggttcgg atagtcagaa cgaccggtgc aaatgatggc atccggacgc
    12121 acttctttcg ccagcggcgg cagaatttcc ggttccgggt tcgccagcgc caggatcatt
    12181 ggcgcacgag ccattttctt caccatttcc tgggtcagca ctttcgggcc ggaacagccc
    12241 aggaaaatat ccgcgccttc aatcacatca tcgagggtac gtttgccgtc atccaccacc
    12301 gcatatgcgg ctttggtttc cgccatgttt ggctcacggc cctgatagat aacgcctttt
    12361 gaatcgcaaa ccacgatgtt atgtttttgc agacccagcg ctaccagcag gttcatacag
    12421 gcgattgctg cggcacccgc gccggaaacc accatccgca cgtcggagat gtttttctcc
    12481 accacgcgca agccgttgag gatggcggca gtgctgataa ttgccgtgcc gtgctgatcg
    12541 tcgtggaata ccggaatatt catccgctcg cgcagtttct gttcaatata gaaacattct
    12601 ggcgctttaa tgtcttcgag gttgatgccg ccgaaggttg gttcgagcgc ggcgacaact
    12661 tcaataaatt tgtccgggtc gagttcgtca acttcaatgt caaatacatc aatcccggcg
    12721 aatttcttaa acagaacgcc cttgccttcc atcaccggtt tgcctgccag cgcgccaatg
    12781 ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg ccaccaggtt accgcgggcg
    12841 gtatatttgt aggcttttaa cgggtctttt tcgatttcaa gacaaggtgc ggcaacgcct
    12901 ggtgagtagg ccagcgccag atcgcgctgt gttgccagag gcttggttgg agaaacctgg
    12961 attttccctg gaactggaaa ttcatggaaa tcaagtgcac tttgttttaa ctggtcatcc
    13021 atttggttgt tcctttcacg taacgttcac aaataaagtg tgtgggcaac agcccctgcc
    13081 cacaacgtgg cgcacattat taccctgccg gagtctacag actttgagca agtccaaacc
    13141 ctcaccatta atataatgtt ttggtaataa tcctataaca ctgatgttac ctgcttaatc
    13201 cagcaatacc atgcctgtct gctatgcttt tttgatgcgt ttagcgaaat ttctcagaag
    13261 tgtgaattaa cgcactcatc taacacttta cttttcaagg agtatttcct atgaacgagt
    13321 tagacggcat caaacagttc accactgtcg tggcagacag cggcgatatt gagtccattc
    13381 gccattatca tccccaggat gccaccacca atccttcgct gttactcaaa gctgccggat
    13441 tatcacaata tgagcattta atagacgatg ctatcgcctg gggtaaaaaa aatggcaaga
    13501 cccaggaaca acaggtggtc gcagcgtgtg acaaactggc ggtcaatttc ggtgctgaaa
    13561 tcctcaaaat cgtacccggt cgcgtgtcaa cagaagttga tgcacgcctc tcttttgata
    13621 aagaaaagag tattgagaag gcgcgccatc tggtggactt gtatcagcaa caaggcgttg
    13681 agaaatcacg cattctgatc aagctggctt cgacctggga aggaattcgc gcggcagaag
    13741 agctggaaaa agaaggtatt aactgcaacc tgacgctgct gttttctttt gcacaggcac
    13801 gggcctgtgc ggaagcaggc gtttttctga tttcgccgtt tgtcgggcgt atttatgact
    13861 ggtatcaggc acgcaagccg atggacccgt atgtggtgga agaagatccg ggcgttaaat
    13921 cggtgcgcaa tatctacgac tactataagc aacaccacta tgaaaccata gtgatgggcg
    13981 cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg ctgcgatcga ctgactatcg
    14041 caccgaattt actgaaggag ctgcaggaaa aagtttcgcc agtggtacgt aaattaatcc
    14101 caccttctca gacgttccca cgcccagctc ccatgagcga agcggagttc cgttgggagc
    14161 acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg cattcgtctg ttcgccgttg
    14221 atcaacgcaa actggaagat cttcttgccg ccaaactata aaccagccac ggagtgttat
    14281 atgtcccgaa aagaccttgc caatgcgatt cgcgcactca gtatggatgc ggtacaaaaa
    14341 gccaactctg gtcatcccgg cgcgccgatg ggcatggctg atattgccga agtgctgtgg
    14401 aacgattttc ttaaacataa ccctaccgac ccaacctggt atgatcgcga ccgctttatt
    14461 ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc tacatctgac cggttacgac
    14521 ctgccgctgg aagaactgaa gaacttccgt cagttgcatt cgaaaacccc aggccacccg
    14581 gagattggct atacgccagg cgttgaaacc accaccggcc cgcttggaca aggtttggcg
    14641 aacgccgtcg ggctggcgat agcagagcgt acactggcgg cgcagtttaa ccagccagac
    14701 catgagatcg tcgatcactt cacctatgtg tttatgggcg acggctgcct gatggaaggt
    14761 atttcccacg aagtctgttc gctggcaggc acgctgggac tgggcaagct gattggtttt
    14821 tacgatcaca acggtatttc catcgacggt gaaacagaag gctggtttac cgacgatacg
    14881 gcaaaacgtt ttgaagccta tcactggcat gtgatccatg aaatcgacgg tcacgatccg
    14941 caggcggtga aggaagcgat ccttgaagcg caaagcgtga aagataagcc gtcgctgatt
    15001 atctgccgta cggtgattgg ctttggttcg ccgaataaag caggtaagga agaggcgcac
    15061 ggcgcaccac tgggggaaga agaagtggcg ctggcacggc aaaaactggg ctggcaccat
    15121 ccgccatttg agatccctaa agagatttat cacgcctggg atgcccgtga aaaaggcgaa
    15181 aaagcgcagc agagctggaa tgagaagttt gccgcctata aaaaggctca tccgcaactg
    15241 gcagaagagt ttacccgacg gatgagcggt ggtttaccga aggactggga gaaaacgact
    15301 cagaaatata tcaatgagtt acaggcaaat ccggcgaaaa tcgctacccg taaggcttcg
    15361 caaaatacgc ttaacgctta cgggccgatg ctgcctgagt tgctcggcgg ttcggcggat
    15421 ctggctccca gcaacctgac catctggaaa ggttctgttt cgctgaagga agatccagcg
    15481 ggcaactaca ttcactacgg ggtgcgtgaa tttggcatga ccgctatcgc caacggcatc
    15541 gagcaccacg gcggctttgt gccgtatacc gcgacgttcc tgatgtttgt tgaatacgcc
    15601 cgtaacgccg cgcggatggc ggcactgatg aaagcgcggc agattatggt ttatacccac
    15661 gactcaattg gcctgggcga agatggtccg acgcaccagg ctgttgagca actggccagc
    15721 ctgcgcttaa cgccaaattt cagcacctgg cgaccgtgcg atcaggtgga agcggcggtg
    15781 ggctggaagc tggcggttga gcgccacaac ggaccgacgg cactgatcct ctcaaggcag
    15841 aatctggccc aggtggaacg tacgccggat caggttaaag agattgctcg tggcggttat
    15901 gtgctgaaag acagcggcgg taagccagat attattctga ttgccaccgg ttcagagatg
    15961 gaaattaccc tgcaagcggc agagaaatta gcaggagaag gtcgcaatgt acgcgtagtt
    16021 tccctgccct cgaccgatat tttcgacgcc caggatgagg aatatcggga gtcggtgttg
    16081 ccttctaacg ttgcggctcg cgtggcggtg gaagcaggta ttgccgatta ctggtacaag
    16141 tatgttggtc tgaaaggggc aattgtcggg atgacgggtt acggggaatc tgctccggcg
    16201 gataagctgt tcccgttctt tggctttacc gccgagaata ttgtggcaaa agcgcataag
    16261 gtgctgggag tgaaaggtgc ctgatggtga ttgccggatg ctgattgccg gatgcgacgc
    16321 tgacgcgtct tatccggcct acagcgtctt atccggccta catgtcccgc cattttgttt
    16381 aacgggtgat ccacaacgtg ggccaggcgt ctggcccatg ccagttatcg caggtgggtt
    16441 ctgcggcgta acgcaccagg cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc
    16501 cacaatcgct taatccgcgc cctttcgcta aggtcaccag ttcacgcgat ttctcatcaa
    16561 atgttgcgtt catcagttcc agttcattcg tctcctgacc gttgttgaac ggcaaacgca
    16621 accgaaccgg gcgcgaagct agtggctttt tacgcgacac aatccatgcc aaatcaatgg
    16681 tgttataggc ccctgcctca cagctaatca tcatcagcgc tttatcatca gtcagcgcag
    16741 tgacattcac ctcacgacgc aatggatcaa gcgagcagcg cagaccattc atccgccagt
    16801 ttccataatc cagcaaatcg ttgcgttctt cgagtgagag tggcgtcggc gttgggttaa
    16861 ccaccgcgac ctctttcagc gcaggcgcgg gcggtacgct gagcggcggt tcgtcccctt
    16921 tcttgatcca cgcggtttca ctgccaacgc gcttttgctg agcatcaata aacaacaacg
    16981 ctgcttttaa gccactcaga gaaatggtct gatcgccatc gcgtaaggtg attgccttcc
    17041 cttcctgaat catctgcaaa aacgcggtga tggttgccgt atcatcggtt actaataacc
    17101 atggtgaaat ccgccacttg tcaccactta acgccaacgg ctcgccatct aacagcagcc
    17161 gtggcgctat ctccccttct gacgcctccg gcgacttcaa tccgccgcgc tcaatacgta
    17221 aaacggcatc ggtatgcgcc ccggcgctgc ggctcagggt catcaccagt ccattatgat
    17281 cgcccgtatt acgcgccacg cagaaatttt ggttattaca ggtgacctgc cagtcggaaa
    17341 acgcccgttg cgctggtgcc gcccacacca aagacgtcgg caacaaagcg aaaaaaaaga
    17401 gaaggaaaat gcgatagcgc atggacggta cgaccccaga agttcaaaca aacagcacaa
    17461 gtcgtatctt cgttgcgcag gtagcgcggc tcaatcggat ttgtcggatt gagccaataa
    17521 taaacaacga aatattattg ttatttttca gtccattaaa tgtgacgttt gcaaatagtt
    17581 aagcaataac accgtcttac cgtcacgtat ctcgccggtt ttgatcatct ccagcgcctg
    17641 gctgaacggc agctcgagca cttcaatatc ttcatcttcg acaccgccac cggcgttagc
    17701 gcgctgattg tcactgtatt cggcgataaa aaagtggatt agctcagtca caccgcctgg
    17761 cgacatatac agttcaaata atttgcgcac ttcacccact tcatagcccg tctcttcaat
    17821 cgcttctttg cgaatacaca cttccggttc gtcgttatcc agcagcccgg cgcagctttc
    17881 aatcagctgc ccgctttcat tgccattaac ccaggtagcg acacggaact gacgaatcag
    17941 aaccacggtc tttttcttcg tgttgtacag gaggatcgtc gcaccattgc cgcgatcgta
    18001 cacttcacgt ttatggcgga taacttcgcc gtctttgcgc gtgagatcgt aagtaatgtt
    18061 gtgcagggtg aaatagttat cggagagaat tttgtcttta atgagggtga tttgttgcgt
    18121 cataccgact ccacagcgcg aaatgaacaa ttatcttacg ctgtgaagtc gggtttgtct
    18181 gcgcaggcta tcagtgagat ttgactgatt ttacccccag ccagtcaata atcccctgtg
    18241 ccgcatgacg tccttctgcc attgcggtaa ccaccagatc cgcaccacgc acggcgtcac
    18301 caccagcgaa gattttcgga ttggtggtct ggtaacggta ctggctttcc acatccgcga
    18361 tgatgcggcc ccatttgtct accgttacac cgtgcgactc cagccacggc atcccgtgcg
    18421 gattgaagcc aaacgccata atcaccgcgt cggctggcat gacaaattca ctgccttcca
    18481 ccggcactgg acgccgacgc ccctgggcat ccggctcgcc aagacgcgtg cgcaggaaac
    18541 gaatcccgca gacgtgacct tgttcattca gctcaagcgc caccggctgg acgttaaatt
    18601 cgaagttggc cccctcttcg cgggcgttct tcacttcttt cttcgagcct ggcatgttag
    18661 cttcatcacg acgataagcg caggtgacgt tactcgcgcc gtggcgcagt gcggtacgca
    18721 cacagtccat cgcggtgtcg ccgccgccca gtaccacgac gttaagtccg gcggtattga
    18781 taaacggctc ttccggtagc tcttcgagcc ccatcacctg tttagtgttg gcaatgagga
    18841 acggcagcgc gtcataaacg cccggcgcat cttcattggg taaacccgct ttcatggaac
    18901 ggtaagtgcc tacgccaacg aagaccgcgt cgtattgttc caaaagcgaa tccaaagaga
    18961 catctttacc cacttcacaa ttgagttcga agtgaatccc catcgcgctg aagatttccc
    19021 gacggcgtgc cagcagggat ttatccagtt tgaaagaagg aatgccgaaa gtgagcaagc
    19081 caccgatttc tggatggcga tcgtacaccg tcacccccac gccattgcgg gtcagaacat
    19141 ccgcacaggc cagccctgcc ggacctgcac cgataatcgc cacccgcttg tccactttgg
    19201 tgacatggct taagtcagga cgccaacctt tcgccaacgc ctgatctgaa atgtagcgtt
    19261 caatgttgcc gatagttacc gcgccgtgct catcgcgaat agtacaggca ccttcacaca
    19321 aacggtcttg cggacaaacg cgtccggtaa tttccggcag ggtgttggtc tggtgagaaa
    19381 gctcgacggc ggcgtcgatg tttccggctt tcaccagttc aatccactgc ggtatatggt
    19441 tatgcagcgg gcaggtccat tcacaaacgc tgtgctcgcc gcacttaagg cagcgcgagg
    19501 cttcccgttg tgcctggtcg gcgcgaaatg gcagataaat ttcatcaaaa ccggttttgc
    19561 gcgcttcaat cgccagttta tccggctcgc cacgcgcggg cgttgcctgc atttgttcga
    19621 ctttactcat taccggcatt tcttgcgccg cggtactggc atgccacggt tgatgttcct
    19681 gacgcgcggt gcgcaagcgg cgggatttcg ccatgccgga gagtgcgacg tcagtgacca
    19741 gttgcagcgc gtccgccggg caattctcaa cacaggcagg accgttttcg cgccccgcac
    19801 aaaggtcgca tttatgcgcc gtggctttta cttttcctgc cgcgacgggt gtcaggacga
    19861 tttgcatcgt accaaaagga caggccacca cgcaggattt acagccaata cacttttgct
    19921 gattgacctg aatgctgtca tcaacgtggc tgattgcgcc attagggcag ctacgggcgc
    19981 agggcgcatc ttcacaatgg tgacaggtca ctgcactacg ttgctgttga tgtttgataa
    20041 ccgtaattcg gggatgaaaa tggtgttggc tcaggacatg ttgctcatca ttgtgagcca
    20101 tgacacaggc gatttcacaa gcatgacaac ccagacattg ctgactgttg gccataataa
    20161 aacgattcat aacgaccttc ttttttggtt gtaaaaacct tattctttat atgagtgttg
    20221 ttattacccg acttacaggg gaatcggcaa tgttcatgtg cccagaataa gtaactattt
    20281 cgctataaac tgtggcagat caaataatcc catcactgac taaattgcgt ttcagcgaac
    20341 tggaacatta atgatttttt gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg
    20401 ccagtctggc ccgggccttt ttttacattg tgctgctgtc gattctttcc acgggtatcg
    20461 ctctgctaac tctggcgagc agtttgcgcg acgctgaggc tatcaatatt gccggatcgc
    20521 tgcgtatgca gagttaccgc ctgggctacg acttgcaaag tggcagtcca caactcaatg
    20581 cacatcgcca gttatttcag caggcactgc attcaccggt attaaccaac ctcaacgtct
    20641 ggtatgtgcc agaagcagta aaaactcgct atgcgcatct gaatgccaac tggctggaga
    20701 tgaataatcg gctcagcaag ggcgatttgc cgtggtatca ggccaatatt aataattatg
    20761 ttaatcagat agacctgttc gtactggctt tacagcacta cgctgaacgc aaaatgctgc
    20821 tggtggtggc gatttccctg gctggcggca tcggtatttt cacgctggtc ttttttactt
    20881 tgcgccgcat acgccatcag gtggttgccc cgctgaatca gctggttacc gccagtcagc
    20941 gtattgaaca cgggcagttc gactcgccgc cgctggatac caacctgccg aatgagcttg
    21001 gtctccttgc aaaaaccttt aaccagatgt cgagcgagct gcataaattg taccgttcgc
    21061 tggaagcgtc agtagaagaa aagacccgcg atctccacga ggccaagcgt cgtctggagg
    21121 tgttgtatca gtgttcgcag gcgctgaaca ctagccagat tgatgtgcat tgtttccgcc
    21181 atattttgca gattgttcgc gacaatgaag cggctgaata tctggagtta aatgtcggtg
    21241 aaaactggcg gattagcgaa gggcaaccaa acccggaatt gccgatgcag attttaccgg
    21301 tgacaatgca agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat
    21361 cagaaccgct gcttaacagc gtttcgtcga tgctgggacg cggtttgtac tttaatcagg
    21421 cgcagaagca ttttcagcaa ttattgttga tggaagaacg tgcgaccatc gcccgcgaat
    21481 tgcacgactc gctggctcag gtactttctt acttacgtat ccagttgacg ttactgaagc
    21541 gttcgatacc ggaagataac gccaccgcac aaagtatcat ggccgatttt tcccaggcgt
    21601 tgaatgatgc ttatcggcag ttacgcgagc tgttgactac tttccgcctg acgctgcagc
    21661 aggcggatct cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg
    21721 ccaaactgac cctcgactgc cgtctgccaa ccctggcgct ggatgcgcaa atgcaggtgc
    21781 atttgttgca aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg
    21841 aaatcgccgt cagttgcgtc accgcgccgg acggcaatca cacggtttat atccgtgata
    21901 acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca ttatggtctg aatatcatgc
    21961 gcgaacgcgc ggaacggctg ggtgggacgc tgactttttc acaaccttcc ggcggcggca
    22021 cgttagtgag tattagcttt cgctctgcgg agggtgagga aagtcagttg atgtaaagcc
    22081 tcctactgac caaagaatac ttgcacttaa ggttcagtat aaaagggcat gataatttac
    22141 attaactcct ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca
    22201 agcctacaac gatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat
    22261 cgccccattt ttgcctgggt gctggcaatc ctgttgtgtc tgacaggtac cctggcgatt
    22321 ttctcattgc ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct
    22381 aactatcccg gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa
    22441 aatatgaccg gcctcgataa tctcatgtat atgtcatctc agagcagtgg caccggtcag
    22501 gcatctgtca ctttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta
    22561 caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg
    22621 acggtgcgta aaaccggcga taccaacatt ctgaccattg ccttcgtctc taccgatggt
    22681 tcgatggata aacaggatat tgctgattat gttgccagta atattcagga cccgttaagc
    22741 cgcgtgaatg gcgtcgggga tatcgatgcc tatgg
//
LOCUS       DACAPB010000060        22751 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.060, whole genome shotgun sequence.
ACCESSION   DACAPB010000060 DACAPB010000000
VERSION     DACAPB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.060"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..455
                     /locus_tag="IT904_002093"
     CDS             <1..455
                     /locus_tag="IT904_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP3191874.1"
                     /translation="LQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRML
                     AGIRIHQGRETDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQ
                     RVTALLSLREVFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            573..1559
                     /locus_tag="IT904_002094"
     CDS             573..1559
                     /locus_tag="IT904_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP3191875.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            1598..2311
                     /gene="lpxT"
                     /locus_tag="IT904_002095"
     CDS             1598..2311
                     /gene="lpxT"
                     /locus_tag="IT904_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAP3191876.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLGFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            2723..3289
                     /gene="mepS"
                     /locus_tag="IT904_002096"
     CDS             2723..3289
                     /gene="mepS"
                     /locus_tag="IT904_002096"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAP3191877.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            3470..5025
                     /locus_tag="IT904_002097"
                     /pseudo
     CDS             3470..5025
                     /locus_tag="IT904_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            5107..6921
                     /locus_tag="IT904_002098"
     CDS             5107..6921
                     /locus_tag="IT904_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP3191878.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVESTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            6922..8016
                     /locus_tag="IT904_002099"
     CDS             6922..8016
                     /locus_tag="IT904_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAP3191879.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            8016..9041
                     /locus_tag="IT904_002100"
     CDS             8016..9041
                     /locus_tag="IT904_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3191880.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            9043..10632
                     /gene="yejF"
                     /locus_tag="IT904_002101"
     CDS             9043..10632
                     /gene="yejF"
                     /locus_tag="IT904_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAP3191881.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILHLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASATHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIILRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(10636..10980)
                     /locus_tag="IT904_002102"
     CDS             complement(10636..10980)
                     /locus_tag="IT904_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191882.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(11313..12503)
                     /gene="bcr"
                     /locus_tag="IT904_002103"
     CDS             complement(11313..12503)
                     /gene="bcr"
                     /locus_tag="IT904_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAP3191883.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(12531..13226)
                     /gene="rsuA"
                     /locus_tag="IT904_002104"
     CDS             complement(12531..13226)
                     /gene="rsuA"
                     /locus_tag="IT904_002104"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAP3191884.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            13375..15135
                     /locus_tag="IT904_002105"
     CDS             13375..15135
                     /locus_tag="IT904_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP3191885.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHVKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRLAHELRG"
     gene            15260..15544
                     /gene="rplY"
                     /locus_tag="IT904_002106"
     CDS             15260..15544
                     /gene="rplY"
                     /locus_tag="IT904_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAP3191886.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(15683..16690)
                     /gene="yejK"
                     /locus_tag="IT904_002107"
     CDS             complement(15683..16690)
                     /gene="yejK"
                     /locus_tag="IT904_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAP3191887.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELKSLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            16872..17099
                     /locus_tag="IT904_002108"
     CDS             16872..17099
                     /locus_tag="IT904_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAP3191888.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            17119..18879
                     /gene="pbgA"
                     /locus_tag="IT904_002109"
     CDS             17119..18879
                     /gene="pbgA"
                     /locus_tag="IT904_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAP3191889.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            18954..19030
                     /locus_tag="IT904_002110"
     tRNA            18954..19030
                     /locus_tag="IT904_002110"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18988..18990,aa:Pro,seq:ggg)
     gene            complement(19133..21724)
                     /gene="yejO"
                     /locus_tag="IT904_002111"
     CDS             complement(19133..21724)
                     /gene="yejO"
                     /locus_tag="IT904_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAP3191890.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSNNTEINGGYQYIEMNGAAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDTTIKGGRLIVEKDGGAVFVAIEKGGLLEVKEGG
                     FAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTVTGVDKKAGGKLIVSTNALEVSGPNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDKHATMQSLGKDTGTKVQANAVYDLGRSYQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLIFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            22044..22691
                     /gene="narP"
                     /locus_tag="IT904_002112"
     CDS             22044..22691
                     /gene="narP"
                     /locus_tag="IT904_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAP3191891.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         5607 a         5831 c         5546 g         5767 t
ORIGIN      
        1 cactgcaaat taaagatgtc gatttaacac aatatgcgga taagttaatt gcacgttttg
       61 ctaatccggc gctgaaacat aagacctggc aaatcgcgat ggatggcagc cagaaattac
      121 cgcaacgcat gctggcaggt attcgcatac atcaggggcg cgaaacggac tggtcgttgc
      181 tggcattagg cgttgcaggc tggatgcgtt acgtcagcgg cgttgatgat gccggaaatg
      241 ccattgatgt tcgcgatccg cttagcgata aaattcgcga acttgttgcg ggcagcagca
      301 gtgaacaacg cgtaaccgcc ctgctttccc tgcgtgaagt tttcggtgat gatctgccag
      361 ataacccgca ttttgtgcag gccatcgaac aagcctggca acaaatcgta caattcggcg
      421 cacatcaggc gctattaaac accctcaaaa tttaacgatt tctgcggtta aagcggatga
      481 agctcacctt cgtccgctct ccccttctct tttctgcctt ttttagccag gattaacgct
      541 cagttaactt accagagtat tgcggagccg ttgtgaccag gaccaacctc atcaccggtt
      601 ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt agcccataaa gatcccaacg
      661 aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg aattgatggt gctttgctcg
      721 ccgatagcgg cgcattgctg aaagagatcc ccggcggctg catgtgctgc gttaatggtt
      781 tacccatgca ggtagggttg aataccttac tgcgtcaggg aaaaccagac cgcttgttga
      841 tagagccgac cgggctgggc catccgaaac agatcctcga tctgttaacc gcaccagtct
      901 atgaaccgtg gatagatctg cgcgccacct tgtgcattct cgatccacgc ctgctgctgg
      961 acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct ggctgccgca gacatcattg
     1021 tcgccaataa atccgaccgt gcgacgcccg aaagtgagca agcgctacag cgttggtggc
     1081 agcaaaatgg tggcgatcga caattaattc acagtgagca tgggaaagtt gacggtcatc
     1141 ttctggattt gccgcgtcgc aatttagccg agttgcccgc cagcgccgcg cattctcatc
     1201 agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa cgctggcgtc
     1261 gcagtctgaa cagcgggcaa ggatatcagg cctgcggctg gatattcgac gctgatacgg
     1321 tattcgacac cattggcatt ctggaatggg cgcgacttgc accggtggaa cgcgtcaaag
     1381 gcgtgctgcg tattcccgaa gggctggtac gaatcaaccg tcagggcgat gacctgcaca
     1441 ttgaaacgca aaacgttgcg ccaccggaca gccgtattga gctgatttcc agcagcgaag
     1501 ctgactggaa tgccttacag agcgcgctgt tgaagcttcg tttagcgact accgcgtaag
     1561 gttgcctgcg tttttcagta agataattag agaaaatatg attaaaaatt tgccgcaaat
     1621 agtgttgttg aatattgtcg gcctcgcgct gtttctttcc tggtatattc ccgttaatca
     1681 tggattctgg ttgccgattg atgcggatat tttttatttc tttaatcaga aactggtcga
     1741 aagtaaggcc tttttgtggc tggttgcatt gaccaacaat cgcgctttcg acggttgttc
     1801 tctgctggcg atgggaatgt tgatgctggg tttctggctg aaagaaaacg cccctggcag
     1861 acgacgtatc gtgattatgg gtctggtcat gctattaact gcagtggtat taaaccagct
     1921 gggtcaggca ttaattcctg taaaacgggc cagcccaaca ttgactttta ccgatattaa
     1981 ccgcgtcagc gaactgctct ctgttcccac gaaagatgcc tcacgagata gctttcccgg
     2041 cgatcacggc atgatgctgc ttattttttc ggcattcatg tggcgttatt tcggcaaagt
     2101 tgcaggcctt atcgccctta ttatttttgt ggtttttgca tttcccagag taatgattgg
     2161 cgcacactgg tttactgaca tcattgtcgg ttcgatgacc gtgatattga tcggtttgcc
     2221 ctgggtgttg ctgacgccat taagtgatcg attaatcacc ttttttgaca aatcactacc
     2281 gggaaaaaac aaacatttcc aaaacaaata actcacagta attaacatca tcaggcttat
     2341 ttttatagtg aggataatcc tgatgatgcg caccgtgctt tcatccatcg cgcgcaaaaa
     2401 tcattctcta agtaaatgaa tggattgcat gcgtttcact caatcgtact ttaattgacc
     2461 aatcccgctt attaactttc tgtatcactt tttcttataa aaaatcatgc aaaaccgctc
     2521 gccaagaccg catcaatcgg gtaatctcga actcgttttg cctcggcggt agattatcct
     2581 cacagcatat aattttgtgc gttagtccac agatttggcc ttaaggaatt gtttcaacat
     2641 gcccaggtaa ttagtctcgt gtcgcttggc atttttttat aacgatattt gtcgttaagg
     2701 acttcaaggg aaaacaaaca acatggtcaa atctcaaccg attttgagat atatcttgcg
     2761 cgggattccc gcgattgcag tagcggttct gctttctgca tgtagtgcaa ataacaccgc
     2821 aaagaatatg catcctgaga cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc
     2881 tcaggatgaa tttgaaaacc tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca
     2941 gtatgctgac tggaaaggcg tacgttatcg tctgggcggc agcactaaaa aaggtatcga
     3001 ttgttctggt ttcgtacagc gtacattccg tgagcaattt ggcttagaac ttccgcgttc
     3061 gacttacgaa cagcaggaaa tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga
     3121 tttagttctg ttccgtgccg gttcaacggg acgccatgtc ggtatttata tcggcaacaa
     3181 tcagtttgtc catgcttcca ccagcagtgg tgttattatt tccagcatga atgaaccgta
     3241 ctggaagaag cgttacaacg aagcacgccg ggttctcagc cgcagctaat aaaccgtttg
     3301 gatgcaatcc cttggctatc ctgacgagtt aactgaaagc actgcttagg cagtgctttt
     3361 ttgttttcat tcatcagaga aaatgatgtt tccgcgtctt gatccaggct atagtccggt
     3421 cattgttatc ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg
     3481 cgctcccaat tttggacgta agctcctgct tacctgcatt gttgcaggcg taatgattgc
     3541 gatactggtg agttgccttc agtttttagt ggcctggcat aagcacgaag tcaaatacga
     3601 cacactgatt accgacgtac aaaagtatct cgatacctat tttgccgacc tgaaatccac
     3661 tactgaccgg ctccagccgc tgaccttaga tacctgccag caagctaacc ccgaactgac
     3721 cgcccgcgca gcgtttagca tgaatgtccg aacgtttgtg ctggtgaaag ataaaaaaac
     3781 attctgttca tctgcgaccg gtgagatgga cattccactc aatgaattga ttccggcgct
     3841 cgacattaat aaaaacgtcg atatggcgat cttacccggc acgccgatgg tgccgaacaa
     3901 acccgcaatc gtcatctggt atcgcaaccc tttgctgaaa aatagcggcg tctttgccgc
     3961 tctgaatctc aacctgacgc cttcactctt ttatagttca cggcaggaag attacgatgg
     4021 cgtcgccctc attattggca atactgcgct atctaccttt tcttcacgtt tgatgaacgt
     4081 taacgaatta accgacatgc cagtccgtga aactaaaatt gcgggcattc ctctgaccgt
     4141 tcggctttat gcagatgact ggacatggaa cgatgtgtgg tacgcatttt tactgggcgg
     4201 catgagtgga actgtcgttg gcctgctctg ctattacctg atgagcgtac gtatgcgccc
     4261 cggcagagaa atcatgacca ccatcaagcg cgaacaattt tacgtggcgt atcaaccggt
     4321 ggtggataca caagctttgc gagtaacggg cctggaagta ctgctacgct ggcggcatcc
     4381 tgtcgcggga gaaattcccc cggatgcctt cattaacttt gccgaatcgc aaaagatgat
     4441 tgtgccgctg actcagcacc tgtttgagtt aattgcccgc gatgccgcag aattagaaaa
     4501 agtgctgccg gtaggcgtca aatttggtat taacattgcg ccggaccatc tgcacagcga
     4561 aagctttaaa gcagatatcc agaaactgct cacttccctg cccgcacacc atttccagat
     4621 tgtgctggaa attaccgagc gcgatatgtt gaaagagcaa gaagccacac aactcttcgc
     4681 ctggctgcac tcggtcggcg tagaaattgc tattgatgac ttggcaccgg gcacagcgcg
     4741 cttatctatc ttgagcgttt tacgctcgat tatctgaaaa ttgaccgtgg atttatcaac
     4801 gccatcggta cggaaacgat cacttcaccc gtacttgacg cggtgctgac gctggcgaaa
     4861 cgcctcaata tgctgacggt tgctgaaggg gtcgaaacgc cggaacaggc gcgatggcta
     4921 agcgaacgcg gcgttaattt catgcaaggc tactggatta gtcgcccgtt accgctggac
     4981 gtttttgttc gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc
     5041 cttattattc atagtgaaag catgccggat tgcggctaat gatgagtaaa aggaaatccg
     5101 ttgcagatga ttgtgcgcat actgctgctg tttatcgccc tgttcacctt cggtgcgcag
     5161 gcgcaggcta tcaaggaaag ctatgccttt gccgtgctgg gcgaaccccg gtacgcattt
     5221 aatttcaacc attttgatta tgtgaacccc gccgcgccaa aaggtgggca gataacgttg
     5281 tcagccctcg gcaccttcga taatttcaac cgctatgcac tgcgcggcaa cccgggcgca
     5341 cgcaccgagc agttgtacga cacgctattt acgacttccg atgacgaacc aggcagttat
     5401 tacccgctga ttgctgaaag tgcacgctat gctgacgatt attcctgggt ggaggtcgct
     5461 attaatccgc gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga tgtagagtct
     5521 acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta caaaggcacc
     5581 accgtcaaag ccattgcacc gttaaccgtg cgcattgagt tagctaaacc cggcaaagaa
     5641 gatatgctga gtctgttttc gctgccggta tttccagaaa agtactggaa ggatcacaaa
     5701 cttagcgacc cgctcgccac gcctccgctt gccagtggtc cgtaccgcgt tacgtcctgg
     5761 aaaatggggc aaaatattgt ctattcccgt gtgaaagatt actgggcagc aaacttaccg
     5821 gtaaaccgtg gacgctggaa tttcgacacc attcgctacg attattacct cgatgataat
     5881 gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc gtatggaaaa cgacgccaaa
     5941 aactgggcca cgcgttatac cggtaaaaat ttcgataaaa aatacatcat caaagatgag
     6001 caaaagaacg aatcagccca ggatacgcgt tggctggcgt ttaatatcca acgtccggta
     6061 ttcagcgatc gccgggtccg ggaagctatc actctcgcct ttgactttga atggatgaac
     6121 aaggcgttgt tttacaatgc ctggagtcgc acgaacagtt attttcagaa taccgaatac
     6181 gcggccagaa attaccccga cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat
     6241 ctaccgtcag aagtcttcac acaaatctac cagccgccgg tatccaaagg cgatggctac
     6301 gatcgtgaca acctgttaaa agccgacaaa cttctcaacg aagcgggctg ggtgctgaag
     6361 ggtcagcaac gcgttaatgc cacaacgggt cagccactca gctttgaatt attgcttccc
     6421 gcaagcagca atagtcagtg ggtattgccg ttccagcaca gcctgcaacg gctgggtatc
     6481 aacatggaca ttcgcaaggt ggataactcg caaatcacta accgcatgcg cagtcgcgac
     6541 tatgacatga tgccgcgcgt atggcgggcg atgccgtggc ccagttccga tttacagatt
     6601 tcctggtcat cggaatatat caattccact tataatgccc ccggcgtgca aagcccggtt
     6661 atcgactcgc tgatcaacca aattattgcc gcgcagggaa ataaagaaaa attactgccg
     6721 ttggggcgag cactggatcg cgtattaacg tggaattatt acatgctgcc aatgtggtac
     6781 atggcggaag accgtctcgc ctggtgggat aaattctccc agccggccgt gcgccccatc
     6841 tatagcctcg gtatcgatac ctggtggtat gacgtcaata aagcggccaa actgccgtcc
     6901 gccagcaaac agggagagta gatgggcgct tacctgattc gccgtctgtt gctggtgatc
     6961 ccaacattat gggcgattat caccatcaac tttttcatcg tgcaaattgc gcctggcggt
     7021 ccggtcgacc aggccatcgc cgccattgag tttggtaatg ccggagtatt acccggcgca
     7081 ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg tcggcaatat cagcgacagt
     7141 aattaccgtg gcggacgcgg attagatcca gaagtgatcg ctgagatcac tcatcgctac
     7201 ggttttgata agccgatcca cgaacgttac ttcaaaatgc tctgggacta catccgcttt
     7261 gattttggcg atagcctgtt tcgcagcgcc tcggtgctga cgctgattaa agacagtctg
     7321 ccggtttcca tcaccctcgg attgtggagc acgctgatta tctatctggt gtcgattccg
     7381 ttaggcattc gcaaagctgt ttataatggg agccgctttg acgtctggag tagcgcattt
     7441 atcatcatcg gctacgccat tccggccttt ttgtttgcca tcctgctgat tgtcttcttc
     7501 gcgggcggca gctatttcga cctgttccct ctacgcggcc tggtttccgc taactttgat
     7561 tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc atatcacgct gccggtgctg
     7621 gcgacagtga ttggtggctt tgcggcgctg accatgctga caaaaaactc attccttgat
     7681 gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag gggtaagtga aaaaaatatt
     7741 ctctggaaac atgtgttccg caacgccatg ctgctggtga ttgccggttt tccggcgacg
     7801 tttatcagca tgttttttac cggctcgctg ctgattgagg tgatgttttc actcaatggt
     7861 ctgggcttac tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc
     7921 ttgtatattt tcaccctgat tggcctgctg ctgaatattg tcagtgatat cagctatacg
     7981 ctggttgatc cgcgtattga ttttgaggga cgttaatgtc gcgactcagc cccgtcaatc
     8041 aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta ctggtcgtta tggattttcc
     8101 tcgtcttgtt tggtttgagt ttgtgttctg aacttatcgc caacgataaa ccgttgctgg
     8161 tgcgttatga cggcagttgg tatttcccgt tattgaaaaa ctacagcgaa agcgattttg
     8221 gcggcccgct ggcaagtcag gctgattatc aggacccgtg gctgaaacaa cggctggaaa
     8281 ataacggctg ggtactgtgg gcaccgattc gctttggtgc taccagtatc aactttgcta
     8341 ccgataagcc cttcccttct ccaccctccc ggcaaaactg gctgggaacg gatgccaacg
     8401 gcggcgatgt gctggcacgc attctctatg gcacgcggat ctcggttctg tttggcctga
     8461 tgctgactct ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa ggttattacg
     8521 gcggtaaagt cgatctctgg ggacaacgct ttattgaagt atggtcgggg atgccgacgc
     8581 tgtttttgat tattttactt tccagcgtcg tacagcctaa cttctggtgg ctgctggcaa
     8641 ttactgtctt gtttggctgg atgagtctgg tcggcgtggt gcgggcggag tttttacgta
     8701 ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg cgtcagcgat cgcagtatca
     8761 tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct caccttttta ccgtttattt
     8821 tatgtagttc gataaccacc ctgacctcgc tcgatttcct cggcttcggt ctaccgctcg
     8881 gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa taaccttcag gccccgtggc
     8941 ttgggatcac cgccttcttg tcggtggcga tattattgtc tttgctgatc tttattggtg
     9001 aagccgtccg cgacgcattt gatcctaata aggcggtgta gcatgacgca aactctgtta
     9061 gcgattgaaa atttgtcggt gggttttcgc catcagcaaa ccgtacgtac agtagtcaat
     9121 gatgtttcac tacagattga ggctggcgaa acgctggcgc tggtgggtga gtcaggttca
     9181 ggcaaaagcg ttaccgcgct gtcaatttta cacctgctcc cttccccgcc ggttgaatat
     9241 ctctccggcg atattcgttt tcatggcgaa tcgctgcttc acgccagcga tcaaacgttg
     9301 cgcggtgtac gcggtaataa gatcgccatg atttttcagg aaccgatggt gtcgttaaat
     9361 ccattgcata ccctggaaaa acagctttat gaagtgcttt cactccaccg cgggatgcgt
     9421 cgggaagcgg ctcgtggcga aattcttaac tgccttgatc gcgttggtat ccgccaggcg
     9481 gcaaaacggc tgacagatta tccgcatcag ctctccggcg gcgaacggca gcgggtgatg
     9541 attgcgatgg cgctgttaac gcgaccggaa ttattaattg ccgatgaacc gaccaccgca
     9601 ctggacgtct ctgtccaggc gcagatttta cagctgttgc gcgaactgca aggcgagctg
     9661 aatatgggca tgctgtttat tactcataac ctcagcattg tcagaaaact ggcccaccgc
     9721 gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaatt acgccgctac gctatttgca
     9781 tcagccactc atccttacac acaaaagcta ctcaacagtg aaccgtcagg cgatccagtg
     9841 ccgttgccag aacctgcctc aacgttgctg gatgttgaac agcttcaggt tgccttcccc
     9901 attcgcaaag ggattttgaa gcgcattgtg gatcataatg tggtggtgaa aaacatcagt
     9961 tttacgctac gagcgggtga aacactgggt ttagtgggcg agtccggttc cgggaaaagt
    10021 acgacgggac tggcgctgct gcgactgatt aattctcagg gcagcatcat ctttgacggt
    10081 cagccactgc aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg
    10141 gtatttcagg atccaaactc ctcgctcaac ccacgactca acgttttgca gattattgag
    10201 gaaggcttac gggttcacca gccgacgctt tctgccgcac aacgcgaaca acaagtgata
    10261 gccgtgatgc atgaagtggg attagatcct gaaacacgcc accgttatcc ggcggagttc
    10321 tctggtggtc agcgacaacg tattgcgatt gccagggcat taattcttaa gccctcgctg
    10381 atcatacttg atgaaccgac atcatcactc gacaaaacgg tacaggcgca aatattgacg
    10441 ctattgaaat cattgcaaca aaagcatcaa ctggcctatt tgtttatcag ccacgatttg
    10501 cacgttgtcc gcgcgttatg tcatcaggtt atcatactgc gacaagggga agtagtggaa
    10561 caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg agtatacgcg tcagctactg
    10621 gcgttgagct gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa ttgccacacc
    10681 aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg cgatttacaa acaggcacgg
    10741 ctcaccttca cactccagca ccgaacatgg aacttcgatg tcgcttaacg cctggcttag
    10801 tttatacatc accgaccatg cattatcacc atccgggcta agcaatttaa gcgccaccag
    10861 gctgttgtca ccgcacggtc cattttgcgg aatcggttca accttcgaac ctgcgaaacc
    10921 agcagaccag cgatagttct gcggcagtcg atggacgatt gagagttgta atgaagtcac
    10981 ttattttccc cggaagcaca ttcacttcac aatttgttta catcaatttt aacacatcat
    11041 caacaatccg tctttataca gatcgtaaag attgatgcct cgcctgcgtg gcctctatgg
    11101 ctctgattta agtaatatct gcgggcgtgg tcacgtttgc ggggctatat gtacccgttt
    11161 ctgcgatcta actcaacctt tttaactaca atgacgtgac tttttacata aattgatttt
    11221 acataaaata aacatatatc gggggaatga tagatttgtg gttgacatgc atcaacaaag
    11281 gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt ttttcggccg actggcgtac
    11341 agacagaaga gaatggagct ggttgcgcag aatgcaattg accaaatcat cggccatgca
    11401 gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg cccctatgcc aaaacggaag
    11461 gttcctgcca gcgaagatgc cgttcccgcc atatggggaa actcatcaag aatgaccgcc
    11521 atcgcattgg atgacaccat cgacacgcag cccacaaacg ccgcaacgcc aaccaccagc
    11581 gaccaaaatc ccagccccag cagcgcactg atgaccatcc acgctgccat aataaattgt
    11641 atccacaacc ccgagcggaa catatttaac gcgccaatgc ggcggacgaa gcggctgtta
    11701 aagatggtca tcacgaacag aaaaacaatg tttagcgcaa agtaataacc aaagttttcc
    11761 ggcgcgacgt ggttaatttc aatataaaca aacggtccgg cgcttaagaa tgagaacatc
    11821 ccggcaaagc tgaaaccact ggcaagcatg tagctcagga cacgtttatg gcggaacagc
    11881 gccgcaaagt taccaatagt ggtacgaatg tgaaatggct gacgacgctc cggtggtaag
    11941 gtttctttaa tcaggaagaa aatcattgcc gaagccagaa tcgccgctaa tgccaggatc
    12001 cagaagatgt aatgccagct cagccacacc agcacccagc cgccaactat cggtgccatc
    12061 agcggtgcaa tggttgtcac cagcatgaca aacgacatca tccgcgagaa ctcttctttc
    12121 gggtaaatat cgcgcatcag ggcgttaatg accacgctgg ccgcagccgc agccagcccg
    12181 tggaagaaac gcatcacaat cagctgatcg atggtgtttg ccaacgcaca cgccaccgcg
    12241 gcggcggcaa acaccagcgt accgccgagc accaccggct tacgcccgaa gctgtctgcc
    12301 atcggcccgt agattaactg ccccaacgca aagcccagaa tataagtact gagggtcatc
    12361 tgcgtactgc ccgccggtac gccaaactgc gctgaaatta ccggtagcgc gggcagatac
    12421 atatcaatcg acagcggcat caacatggcc agcaggccaa ggataaaaac aatagcaaac
    12481 gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt cattgaaaag ttagacgacg
    12541 ctggcaattt cttcttcagt taacggacga tattcaccgg gggctaaatc agcatccagc
    12601 gtaataccgc caatacgttc acgatgcagc tcaaccacgt ggttacccac ggcggcgaac
    12661 atgcgtttca cctgatgata acgcccttcg ctgatggtca gacgaacctg cgttggggta
    12721 atcacttcca gcaccgcagg cttagtgaga tctttttcgt tatgcagctg cacgccttta
    12781 gcaaattgct ctgccgtatc gtcagctaca ggtgattcca gtgtcaccag ataggtcttc
    12841 tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact gaccatcatc agtcatcagc
    12901 accagaccgg tggtatcaat atccaaccgc cccgccgcat gcagtttcca cgctaccggt
    12961 tcatcaagaa aatagagcac cgttgggtga tcagggtcgt ccgtggagca aacatagccc
    13021 tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct gcgccagcgg gttgccatcg
    13081 taagcgacat catgttcagg aagcagtttg aacgctgcat tacggacgat ttcgccatcg
    13141 acggtgacac gattgccgcg gatttcacgc ccggcaatag cacggctaac gccgagttgc
    13201 tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc ctgtaaaaac ggaggtcggg
    13261 cattgacccg aaaatctgaa ctgttgtctg cccagtatag cggtctaata acatccctca
    13321 agggaaaaag atccatggca tactattagc agaataatct acctacgcga gaccatgatt
    13381 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    13441 cataaaaccc ctgccgttat cgtgctgccc accggcgcag gtaaaagcct ggtgatagcg
    13501 gaactggcac ggctggctcg tggtcgcgtg ctggtgctgg cacacgttaa agaactggtg
    13561 gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgccggg
    13621 ctaaagcgca aagagagcca cgttaaagtg gtatttggca gcgtgcagtc tgtcgcccgt
    13681 aatcttgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt
    13741 ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgacaaa agtgaatccc
    13801 cacttacgcc tgctggggct gactgccacg ccttttcgat tgggcaaagg ctggatctac
    13861 cagtttcatt atcacggcat ggtacgcggc gatgagaaag cccttttccg tgactgcatt
    13921 tatgagctgc cgctgcgtta tatgattaaa cacggctatc tgacgccgcc agaacgactg
    13981 gatatgccag tagtgcaata cgatttcagc cgcttgcagg cacagagtaa cgggctgttc
    14041 agcgaagccg atctcaaccg tgagctgaaa aaacaacaac gtattacccc gcacatcatc
    14101 agccagatta tggagtttgc tgcaacgcgc aaaggggtga tgatttttgc cgcgacggtt
    14161 gaacacgcaa aagagattgt gggattactg cctgccgaag atgcagcact gattactggc
    14221 gacacccccg gcgctgagcg cgatgtgtta attgaaaatt ttaaagccca gcgttttcgc
    14281 tatctggtca acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    14341 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    14401 cgtctcgctc cgggcaagac tgattgctta attcttgatt atgcgggtaa tcctcacgat
    14461 ctctacgcgc cggaagttgg tacaccaaaa ggcaaaagtg acaacgttcc ggtacaggtt
    14521 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    14581 ttgattgaac actttggtcg tcgctgtcag ggatggtttg aagatgacga cggtcatcgc
    14641 gaacaatgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc ggaaaacgat
    14701 attgccgccc gccgctgccg cgaatgtgac accgtactgg ttgatccgga cgatatgtta
    14761 aaagcggcgc tacgactgaa agacgcgctg gtattacgct gtagcggcat gtctttgcaa
    14821 catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggcgcg
    14881 gatgtgagtg agcgtttccg tctgcaaaca cctgcccagc gtaccgcctt cgagcagctt
    14941 tttatccgcc cgcatacgcg cacaccgggc atcccgctgc gctggatcac cgccgccgat
    15001 atcctcgccc agcaagcctt attgcgacac ccggattttg tcgtcgcccg catgaaaggc
    15061 cagtactggc aggtgcgtga aaaagtgttc gattacgaag gtcgttttcg tctggcgcac
    15121 gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta tagaatctcg
    15181 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    15241 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    15301 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    15361 agaagcgccg ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc
    15421 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    15481 tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc
    15541 ttaattgctg aataagttgt aaaaaacccc gctccggcgg ggttttttgt atctgcagat
    15601 tatgcctgat tacggtattg ctattttttg caggccagat aaggcgttca cgccgcatct
    15661 ggcattcaga acacaaaacc gattaattgc cgccagacgt ccggcgctgc aattggtcgc
    15721 gcaaattcgg cggcgtgcct ttaatggtca aggtatcggt tgctggatcc cagaagatgc
    15781 gttcacctaa cagcatcgca tcaaagttaa tagtcaagcc accgccgcta ccggcaaact
    15841 tcgtcaactg acgcagtgtg ctacggtctg ccgggaagct ctcttccagt tcgtagcctt
    15901 tttcagccgc aaactccgtg aagctcactt cgcttacgcc tgccagctct ttcgacagcg
    15961 atttcagttc gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct
    16021 ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg
    16081 cttgcaacag cccacggttt tgcgctttcg cgtttaaccc ttcgctggcc ccgaggaaat
    16141 ccataaagaa atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg
    16201 tggactctgg attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat
    16261 tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacgcgc atactgctca
    16321 ggttgctcag aactgccacc agcaaatact ccaccgccag atagcgatag tggcaaaaca
    16381 gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg cggccggttg
    16441 ccgcgcggct aaatgccagg aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg
    16501 ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat
    16561 gcagttctgc caccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca
    16621 ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat
    16681 ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc
    16741 gacgcaataa aagatggtgg tcattttaca gaaggcagcg taacgagaat gatttaacgc
    16801 ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact
    16861 gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg
    16921 cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt
    16981 taggcaacat ggtcactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa
    17041 ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag
    17101 ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag
    17161 atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc
    17221 agccgttacc tgtttatcgc cgactggccg acaacgcttg ctggtcgcat ttattcctac
    17281 gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc
    17341 ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca
    17401 acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat
    17461 cttaatccca tcgtctggca actggttatc aacccagacg aaaatgagat ggcgcgcgac
    17521 tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg
    17581 tggagctggc aaaagctgcg cagcctgacg cgtcgtcgac gcttcgcgcg cccgctggcc
    17641 gcattcttat ttatcgcctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac
    17701 ttctatcgcc cgatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg
    17761 cgacgttttc ttgagaagca tggtctgctt gatgcgcagg agtatcaacg ccgtcttatt
    17821 gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc
    17881 gatatgggca ccgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc
    17941 ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc
    18001 catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta tggcatctcg
    18061 ccgagctata tggacggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt
    18121 aatcagcaag gctatcagct ggggttattc tcatcagatg gctttaccag cccgctgtat
    18181 cgccaggcat tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag
    18241 accgccacgc agtggatcaa ctggctggga cgctacgcac aagaagataa ccgctggttc
    18301 tcgtgggttt ctttcaatgg tactaacatt gacgacagca atcagcaggc atttgcacgg
    18361 aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg
    18421 cgtgattctg gcaaactgga caatacggta gtgattatca ctgccggtcg gggtattcca
    18481 ctgagcgaag aggaagaaac ctttgactgg tcccacggtc atctgcaggt accattagtg
    18541 attcactggc caggcacgcc ggcgcagcgt attaatgctc tgacggatca taccgatctg
    18601 atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa
    18661 ggtcaggatt tgttcaaccc tcaacgccgt cattactggg ttaccgcagc ggataacgat
    18721 acgctggcaa ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc
    18781 acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg
    18841 caagtgctga cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg
    18901 aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt agcagccacg agtcggcacg
    18961 tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc
    19021 gtgccgacca aaaatcccaa gaaaaaacca acccttacgg ttggtttttt tatatctgca
    19081 attaattcga taaacagacc gtgacacatc acagcctgtt tattttctgt tatcagaacg
    19141 tccagaccac acccgcctga gtattccacg gcgattctac ccctgcgcca ttgccatagc
    19201 tgactgacaa atgaccgctt aacgtcgggg taaacgatga ccttatccca gcctgataaa
    19261 cgccactggt tccagccaca tcattattaa atttgccatc gtcattaact ttcacctggt
    19321 tagaatcggc gtattcctga cgcacggccg ctttcagcca gggttccagc gtcgtaccgt
    19381 tttgcaggtc catgtgatag cttaccgccg ttcccgcttc agcgcgtaat atccgggtat
    19441 ttcccacatc cgcgcgcatg ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc
    19501 cggtaaaggc cagatagggt ctaacactcc acaatccgtc aacccaacgg aacccgctct
    19561 caacatgagc acccgcgccg ttactattgt aatcgccaaa cgctgttgcc ccattactca
    19621 tcttgccatg gatggtgttg gcaaaacggt caactttcac caccccatca acataggcac
    19681 cgttctgatg ctcccaaccg gcataagccc ccagggtata gctatcgata ttacctttgc
    19741 cgccgcgatc aaaaccaata tcagaatgag agtaaccaaa gatcaagccg cgaattgtac
    19801 tgctttcttc acgggagaaa cggctatcga taccgagcgt caggcccgtc aatgtttgct
    19861 caaaaccagc tcccgcatca gtggtcacgt tgttgcgggt gttaattgcc gaactccaca
    19921 tcgccgtatc gtaactaacg ccttttacgc taccaagacg ctcacgcacg gtgtccagtt
    19981 ctgcatcaaa taccagcggt tgtgcggccg ccatattcag cacatcagtg gttgaagggg
    20041 taatttgcgc gcgattctct gccagactcc agctatggtt gccattatcc agcaaggtat
    20101 attcatacgt accgatatca acaacgcctc cggcattgcc caacgtaaat gcagcatcac
    20161 cgccgcccgt tgttaccagt gtaaggctat ctcctgctgc cgggctggca ccggtgtccg
    20221 tcacgaatat tttgaaatca cctgttgcct gaccggtgac gttgagctga tcgctctgat
    20281 gattagccat atcggtacgc atataaaaat tgccgtttcc cgacagggta ttggtggtca
    20341 acgtaatgaa attttccgca cttgccgtca ctgatgaacg agtcactggc tcatccatca
    20401 taatcacatt tgcgtcagac atcgcaaggt tggctaagtt gaggagggtg ttttggttcg
    20461 tcgtagtgat atcggcaatg atggtccata cgctattttc gagcgaaacg tccgctttgc
    20521 tggtatccac ggcaccatgc gttctaaaag aagcgccctc agaaaccact accttaccca
    20581 ccaacattgc gggtgcataa tttatttgcg gcttcatgac ctcaagaatg ccatcattcc
    20641 ctctgactga aacgttaacc attgtgccag cccagacgtt agcgcggcca ttgttgataa
    20701 ccatattttc agagatggct tttgaggaat acgtgatact tccattctga tatgatcgac
    20761 cgagatcata taccgcattt gcctgcactt tcgtaccagt atcctttccc agcgattgca
    20821 tagtggcatg cttatcaagg atagtatcaa tggcctgcgt gtcctccata acaatgagcc
    20881 cggaaccatc atccagttca taattttttg acacaccatc ttttatacta aattggcctt
    20941 tactgtttgg accactcact tccagcgcat tcgttgagac aattaatttt ccgcctgctt
    21001 ttttatcaac gccagttaca gtcccgccat ccataacctc cagtaagccg ccactatcta
    21061 cagtggtatt ataagcgaag tcattttctt caactcgcaa acttccgccg ttttccaaca
    21121 acatattatt agcaatacca ttcttgatat cgaactgacc gagacggttt gttccgaata
    21181 cctccatggc ccgcgtggtt gttttaatag caccgcctgc tttctgatct accgcaaatg
    21241 caaatccccc ctctttaacc tccagtagtc ctcccttttc gatagcgaca aagacggccc
    21301 ccccatcttt ttcaacgatt aagcgcccgc ctttaatcgt ggtatcattc gctgcgccat
    21361 aaacctgtag cacaccatta ttgagcgtag tctggtttgc cgcgccaccc atttgaacaa
    21421 tttgataacc gtcatttaat actgagtatt ctgcggcgcc attcatttca atgtactgat
    21481 accctccgtt aatttcagta ttattactta caccaaattc ttttatatgc tgttctccgc
    21541 caggattgat tttcgtatta ttggcggtcc cataaaccag ctggatatct ttttccagga
    21601 caacaccatc aactgtctca ccatactcaa ctgttgatgc caatgctatg ggtgaatata
    21661 acgctgtagc caccagaaca gatattgcgg aacgacaaag agaaacagaa ccagattgat
    21721 gcattgagct ttcatcctat gaaattaatt gcagttaaaa gcattgggta cagaaaatac
    21781 ccatagctcc atacccggag tcagttttta aaaactgttt aaagaaatgc acaagtattg
    21841 tgattgattt tttagttgtt tttcttgatg agaagctgat gcaaaattcc gtctttataa
    21901 tgaaaatgat gccaaagcga acgacaaggt tgtagttttc actacatgtc catacataaa
    21961 atggggtaac attcacgcgc ctggtagcgt taccaacgct acgctcaaac ataatgattc
    22021 taataaaacc tcaggagact actatgcctg aagcaacgcc ttttcaggtg atgattgtgg
    22081 atgatcatcc acttatgcga cgcggtgttc gtcagttact ggagcttgat cctggctttg
    22141 aagtggtcgc cgaagcgggc gacggcgcga gcgcgatcga tctggcgaat agactggata
    22201 tcgacgtgat cttgctggat ctcaatatga aaggtatgag tggcctggat accctcaatg
    22261 ccttgcgcag ggatggcgtt accgcgcaaa ttattatcct gaccgtatca gatgcctcca
    22321 gcgatgtctt tgcgctgata gacgcaggcg cagacggtta tctgttgaaa gacagcgacc
    22381 cggaagtatt gctggaagca attcgtgctg gagcgaaagg cagcaaagtc tttagcgaac
    22441 gcgtcaatca gtacttacgt gaacgtgaaa tgtttggcgc ggaagaagat cccttcagcg
    22501 tgctgacgga gcgcgagctg gatgttctgc acgagctggc acaggggctg tcaaataaac
    22561 agattgcctc ggtgttgaat atttcagagc agacagtaaa agtacatatt cgcaatctgc
    22621 tgcgcaaact caatgtccgc tcacgcgtgg cggccaccat tctgttcctg caacaacgcg
    22681 gggcacaata aaaatagccc gatggattta tcatcgggct gagatttatg acaaacgcaa
    22741 aactgcctga t
//
LOCUS       DACAPB010000061        22568 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.061, whole genome shotgun sequence.
ACCESSION   DACAPB010000061 DACAPB010000000
VERSION     DACAPB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.061"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..550
                     /locus_tag="IT904_002113"
     CDS             <1..550
                     /locus_tag="IT904_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191892.1"
                     /translation="PDTNSFLLTNKEYLYGKFGINGWQKYVRFLRAFVDAYKNSDMIY
                     TYLPGGDNFTLSADNRIFIPILGLGLINAVNERDVIIVKQWRKHEGYRYLPCFDILKI
                     QNKFYVKIKYKEDVFFLRKNKDLLQELSGTIDVIVGSRFIKELKESRSFSRVEISESE
                     LWGISNNPVNHAHQRNPNKKWS"
     gene            738..1106
                     /locus_tag="IT904_002114"
     CDS             738..1106
                     /locus_tag="IT904_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HAP3191893.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDLMTEEKLHKINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPTMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            1179..1343
                     /locus_tag="IT904_002115"
     CDS             1179..1343
                     /locus_tag="IT904_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAP3191894.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            1312..1455
                     /locus_tag="IT904_002116"
     CDS             1312..1455
                     /locus_tag="IT904_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAP3191895.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKCILMLKLRSSK
                     SIH"
     gene            1531..1827
                     /locus_tag="IT904_002117"
     CDS             1531..1827
                     /locus_tag="IT904_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAP3191896.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1833..2618
                     /gene="bet"
                     /locus_tag="IT904_002118"
     CDS             1833..2618
                     /gene="bet"
                     /locus_tag="IT904_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HAP3191897.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2615..3295
                     /locus_tag="IT904_002119"
     CDS             2615..3295
                     /locus_tag="IT904_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HAP3191898.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDENMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3321..3725
                     /locus_tag="IT904_002120"
                     /pseudo
     CDS             3321..3725
                     /locus_tag="IT904_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3741..3817)
                     /locus_tag="IT904_002121"
     tRNA            complement(3741..3817)
                     /locus_tag="IT904_002121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3781..3783),aa:Arg,seq:tct)
     gene            4060..4692
                     /gene="fimZ"
                     /locus_tag="IT904_002122"
     CDS             4060..4692
                     /gene="fimZ"
                     /locus_tag="IT904_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAP3191899.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(4695..5210)
                     /gene="sfmF"
                     /locus_tag="IT904_002123"
     CDS             complement(4695..5210)
                     /gene="sfmF"
                     /locus_tag="IT904_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAP3191900.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(5221..6228)
                     /gene="fimH"
                     /locus_tag="IT904_002124"
     CDS             complement(5221..6228)
                     /gene="fimH"
                     /locus_tag="IT904_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HAP3191901.1"
                     /translation="MKIICRLLLAMACLWLTNISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHD
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINIV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(6241..8850)
                     /locus_tag="IT904_002125"
     CDS             complement(6241..8850)
                     /locus_tag="IT904_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAP3191902.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(8881..9573)
                     /gene="fimC"
                     /locus_tag="IT904_002126"
     CDS             complement(8881..9573)
                     /gene="fimC"
                     /locus_tag="IT904_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAP3191903.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGNQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(9793..10335)
                     /gene="fimA"
                     /locus_tag="IT904_002127"
     CDS             complement(9793..10335)
                     /gene="fimA"
                     /locus_tag="IT904_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAP3191904.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            10816..11682
                     /gene="folD"
                     /locus_tag="IT904_002128"
     CDS             10816..11682
                     /gene="folD"
                     /locus_tag="IT904_002128"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAP3191905.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            11684..11896
                     /gene="ybcJ"
                     /locus_tag="IT904_002129"
     CDS             11684..11896
                     /gene="ybcJ"
                     /locus_tag="IT904_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAP3191906.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAISEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            12004..12525
                     /locus_tag="IT904_002130"
     CDS             12004..12525
                     /locus_tag="IT904_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP3191907.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVFIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(12561..13946)
                     /gene="cysS"
                     /locus_tag="IT904_002131"
     CDS             complement(12561..13946)
                     /gene="cysS"
                     /locus_tag="IT904_002131"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP3191908.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTIWRRK"
     gene            14120..14614
                     /gene="ppiB"
                     /locus_tag="IT904_002132"
     CDS             14120..14614
                     /gene="ppiB"
                     /locus_tag="IT904_002132"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAP3191909.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            14617..15339
                     /gene="lpxH"
                     /locus_tag="IT904_002133"
     CDS             14617..15339
                     /gene="lpxH"
                     /locus_tag="IT904_002133"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAP3191910.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRKMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            15457..15966
                     /gene="purE"
                     /locus_tag="IT904_002134"
     CDS             15457..15966
                     /gene="purE"
                     /locus_tag="IT904_002134"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP3191911.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            15963..17030
                     /gene="purK"
                     /locus_tag="IT904_002135"
     CDS             15963..17030
                     /gene="purK"
                     /locus_tag="IT904_002135"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP3191912.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGAHGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(17169..18062)
                     /locus_tag="IT904_002136"
     CDS             complement(17169..18062)
                     /locus_tag="IT904_002136"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP3191913.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSCYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(18059..18874)
                     /locus_tag="IT904_002137"
     CDS             complement(18059..18874)
                     /locus_tag="IT904_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP3191914.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(18885..20144)
                     /locus_tag="IT904_002138"
     CDS             complement(18885..20144)
                     /locus_tag="IT904_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP3191915.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWVKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(20154..21821)
                     /locus_tag="IT904_002139"
     CDS             complement(20154..21821)
                     /locus_tag="IT904_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP3191916.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            22138..>22568
                     /locus_tag="IT904_002140"
     CDS             22138..>22568
                     /locus_tag="IT904_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="catalyzes the formation of oxalurate from
                     ureidoglycolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAP3191917.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMV"
BASE COUNT         5762 a         5793 c         5506 g         5507 t
ORIGIN      
        1 tcctgataca aatagttttc ttctaacaaa taaagaatac ttatatggta aatttggtat
       61 aaacggatgg caaaaatatg tacgattctt acgtgcattc gttgatgcgt ataaaaattc
      121 cgacatgatt tatacatatt tgcctggtgg tgataatttt acgctttcgg cagacaatag
      181 aatattcata cctatacttg gtcttgggct gattaatgca gtaaatgaaa gagatgttat
      241 aattgttaag cagtggcgaa agcatgaggg atatagatat ctgccatgct ttgatatatt
      301 aaaaatacag aataaattct atgtaaaaat taaatataaa gaagatgttt tcttcctcag
      361 aaaaaacaaa gatcttttac aagaactttc tgggacaata gatgtcatcg taggttctag
      421 gtttataaaa gaactgaaag aaagcaggtc tttctctcgt gtagagataa gcgagtcaga
      481 gttatggggt atatcaaata atcctgtaaa ccacgctcac caacgaaatc caaacaaaaa
      541 gtggtcataa cccgctcaag cgggtttcat tttcacgcaa acaaaagaat aaacactgca
      601 ctgtgtattc attccaacga gtgaatacac ggagcaatgt cgctcgtaac taaacaggag
      661 ccgacttgtt ctgattattg gaaatcttct ttgacctcta atgtgagggc gatttttttc
      721 tgtgaggata tgaacagatg tcaaacatca aaaaatacat cattgattac gactggaaag
      781 catcaataga aattgaaatc gaccatgact taatgacaga ggaaaaactt cacaagatta
      841 ataatttctg gtcagactct gaataccgac tcaataaaca cggctctgta ttaaatgctg
      901 tattaatcat gctggcgcaa catgctctgc ttatagcaat ttcaagcgac ttaaatgcat
      961 atggtgttgt gtgtgagttc gactggaatg atggaaatgg tcaggaagga tggcctacaa
     1021 tggatggtag cgaaggaata agaattaccg atatcgatac atcaggaata tttgattcag
     1081 atgatatgac tatcaaagcc gcctgagcgc ggcgttaccg cataccaata acgcttcact
     1141 cgaggcgttt ttcgttatgt ataaataagg agcacaccat gcaatatgcc attgcagggt
     1201 ggcctgttgc tggctgccct tccgaatctt tacttgaacg aatcacccgt aaattacgtg
     1261 acggatggaa acgccttatc gacatactta atcagccagg agtcccaaaa aatggatcaa
     1321 acacttatgg ctatccagac taaattcact atcgccactt ttattggcga tgaaaagatg
     1381 tttcgtgaag ccgtcgacgc ttataaaaaa tgtatattaa tgctgaaact gagatcaagc
     1441 aaaagcattc actaaccccc tttcctgttt tcctaatcag cctggcattt cgcgggcgct
     1501 attttcacag ccattttcag gagttcagcc atgaacgctt attacattca ggatcgtctt
     1561 gaggctcaga gctgggcgcg tcactaccag cagatcgccc gtgaagagaa agaggcagaa
     1621 ctggcagacg acatggaaaa aggcctgccc cagcacctgt ttgaatcgct atgcatcgat
     1681 catttgcaac gccacggggc cagcaaaaaa gccattaccc gtgcgtttga tgacgatgtt
     1741 gagtttcagg agcgcatggc agaacacatc cggtacatgg ttgaaaccat tgctcaccac
     1801 caagttgata ttgattcaga ggtataaaac ggatgagtac agcactcgca acgctggcag
     1861 ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg atcaccactc
     1921 ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca gttcatcgca ttgttgatcg
     1981 tcgccaacca gtacggcctt aatccgtgga cgaaagaaat ttacgccttc cctgataagc
     2041 agaacggtat cgttccggtg gtgggcgttg atggctggtc ccgcatcatc aatgaaaacc
     2101 agcagtttga tggcatggac tttgagcagg acaatgaatc ctgtacatgc cggatttacc
     2161 gcaaggaccg taatcatccg atctgcgtta ccgaatggat ggatgaatgc cgccgcgaac
     2221 cattcaaaac tcgcgaaggc agagaaatca cggggccgtg gcagtcgcat cccaaacgga
     2281 tgttacggca taaagccatg attcagtgtg cccgtctggc cttcggattt gctggtatct
     2341 atgacaagga tgaagccgag cgcattgtcg aaaatactgc atacactgca gaacgtcagc
     2401 cagaacgcga catcactccg gttaacgatg aaaccatgca ggagattaac actctgctga
     2461 tcgccctgga taaaacatgg gatgacgact tattgccgct ctgttcccag atatttcgcc
     2521 gcgacattcg cgcatcgtca gaactgacac aggccgaagc agtgaaagct cttggattcc
     2581 tgaaacagaa agccactgag cagaaggtgg cagcatgata ccggacatta tcctgcagcg
     2641 taccgggatc gacgtgagag ctgtcgaaca gggggatgat gcatggcaca aattacggct
     2701 cggcgtcatc accgcttcag aagttcacaa cgtgatagca aagccccgct caggaaagaa
     2761 gtggcctgac atgaaaatgt cctacttcca taccctgctg gctgaggttt gcaccggtgt
     2821 ggctccggaa gttaatgcta aggcgctggc ctggggaaaa cagtacgaga acgacgccag
     2881 aaccctgttt gaattcactt ccggcgtgaa tgttactgaa tccccgatca tctatcgcga
     2941 cgaaaatatg cgcactgcct gctctcccga tggtttatgc agtgacggca acggccttga
     3001 actgaaatgc ccgtttacct cccgggattt catgaaattc cggctcggtg gtttcgaggc
     3061 aataaaatcg gcttacatgg cccaggtgca gtacagcatg tgggtgacgc gaaaagatgc
     3121 ctggtacttt gccaactatg acccgcgcat gaagcgtgaa ggcctgcatt atgtcgtgat
     3181 tgagcggaat gaaaagtaca tggcgagttt tgacgagatg gtgccggagt tcatcgaaaa
     3241 aatggacgag gcactggctg aaattggttt tgtatttggg gagcaatggc gatgacgcat
     3301 cctcacgata atatccgggt accgcctgta aagtgttgcg tgatcaaata ggcaagcatc
     3361 acaaatgggt gtttgtacat accaaggcgg ctaagcgagc agatggaaca tcaacgcctg
     3421 cggtcaggaa gatgcgcatc gacagcaaga catcatggct atcagcttgt cgtcgtgcag
     3481 gaattgaaga tttccgtttc catgacctca gacacacctg ggcaagctgg ctgattcagt
     3541 caggcgtccc attatcagtg cttcaggaaa tgggcggatg ggagtccata gaaatggttc
     3601 gtaggtatgc tcaccttgcg cctaatcatt tgacagagca tgcgaggaaa atagacgaca
     3661 tttttggtga taatgtccca aatatgtccc actctggaat tatggaggat ataaagaagg
     3721 cgtaactgat tgaattgtaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt
     3781 agaaggtcgt tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa
     3841 tcgcgtgaat tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac
     3901 aagtgaacga ccgcgtctga ttttctgatt tatttcgcta tagtggcaaa caaacgcaca
     3961 ccgctgcgcg tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc
     4021 cctaacggaa aattgtccgc tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc
     4081 attatggata ctcatcctat catcagaatg tctattgaag ttctgttgca aaaaaacagt
     4141 gaattgcaga ttgtcctgaa aacggatgat taccgcataa ccatcgatta tctccgaacc
     4201 cgtcctgttg atttaatcat tatggatata gacttgcccg gaacagacgg ttttaccttc
     4261 ctgaaaagga tcaaacaaat ccagagcaca gtgaaagtgt tatttttatc atcgaaatca
     4321 gaatgctttt atgctggcag agcgatacaa gctggtgcta acggttttgt cagtaaatgc
     4381 aatgatcaga atgatatttt tcatgccgtt cagatgatcc tctccggata cacgtttttt
     4441 cctagcgaaa cgcttaacta tataaaaagc aataaatgta gtacgaatag ttcaacgatc
     4501 actgtgctat ctaatcgtga agtgaccata ttacgttatc tggttagcgg attatctaat
     4561 aaagaaattg ccgataagtt attacttagc aataaaacag ttagtgcgca taaatctaat
     4621 atttatggca agctaggttt gcattcaatt gtagagctta tcgactacgc caaattatac
     4681 gaattaatat aatattaatt ataattgatc ataaatatcg catccgcttt cgccacacct
     4741 ggccgaacac cgctggctaa cgctcgataa ttggcaaaaa agtttagtgt gacattgcca
     4801 tttgcatcta cttcctcagt cgggctcgcc tcccccagtg cgagccggga gcgatcgcta
     4861 ttacgtaatt cgatggcgac ggtttgtgcc attgcgggat catccagagc cagcaggttg
     4921 gtatcggatg ccggcgttcc cgtaaataaa atcgcaactg aacccggagg acatccctcc
     4981 agccgcaggc taaaagggac gagtgccgtg gtatcgccag cgttcagtaa ttgtgtcgta
     5041 ggccatctgc ctaaatctac cgtcttatca atatccgctg tgtttacggt acaggagaaa
     5101 tcaacaacgt taccgtgcaa attgatatta atcgttccta aagggtcagc tgcccatcca
     5161 ctggaactcc acagtaaccc gcagaaacag ctaaagagta ctcttctcat tctccgtacc
     5221 tcattgataa tcgacgcgta aataccccag cgcgctaaac ggcccctcgg tcggtttttg
     5281 accggtaata ctgataggcc aggcgcgaag tgtgacattg gctgccgcag ctgcatccag
     5341 acggaaagga ataacgctat tgagatcgtt aggcgtaatc ggcgtatcgt tctgatcggc
     5401 gacaataaaa cctaaatcct gattgtccga caccatcgcc tgaccagaaa cggcactggc
     5461 ttccagacgc attgttaaat aagcctgcgc agcaacattc gtacatttga ccgcaatgct
     5521 cttggtttgc ggcatgacac cagcaggtcg attacccggc cctgccgcac taaataacga
     5581 tgcgccgata tcaccaaaat caaattcaac aatctgcccg gcatttaatt cgcagttttg
     5641 cggtacttca acccggccac caaaactaat ggtataaaca ggtgtaacca acggatcgta
     5701 aggcgttgat gtcacataca cggtaaacat cacctgtctg gggatctcca ctatgttaat
     5761 aaagggacga ataaccttta aacgaaatat taaacgagaa tccatcaccc cgaatggaag
     5821 gttttgcgaa acgttaggat ctgttcccat ccgaatataa ttttgcgggg ggaactgaat
     5881 atccataaca ctatcaacca gactcatcgc acctaataga taatcatgta attgcatata
     5941 tttataatta tcgatagttt cctgaacaat aaagttggtg acataactac ggtatgtata
     6001 attcaccagc gtgccgggtg gacaaattgc tgacacacct acccagccgg atttttccgg
     6061 taaaacaaca atctgccctg gctggttatt ggtgctatta aagacatttg agagatcata
     6121 gtgttcatct tctgctacgc cagtactatt tgcacaaaca gtagcccagg atatgtttgt
     6181 taaccacaaa catgccatcg ccaataataa tctgcagatt atcttcattg ataatttcct
     6241 ttatgagcca ggatgtgtgc aaactgccga aataactgtg acggcttgct gtaagctttg
     6301 cttcggcaat acatagtgcg caatgcagcg tgagttcgcg ccgtctcccc actgaaccag
     6361 taattcacca gacaatggcg caccacttaa ataaacttgc ccgtcatcac ccaccatact
     6421 ggttattccg gtactgtttt cccgtaccag tgatccaaac ggcaccggtt ttctgccctg
     6481 ggtaacggta atgagcgccc gcacgcctat gcgggtatca aaattggcac gaaccaacgc
     6541 gccttgcgtc ggcacaacgc tgctaatatt tttttcaaca tcgatggaat tccccatcgt
     6601 attggtatca agcgcgatac ggttataccg ataaaccgtg gcatacggca tcaccgcata
     6661 gccgcgccag tcggttaaaa tgccagtttg attttcaatg cgtacaccgc ctgcgccagg
     6721 cgctttaatc aaaacattgg tatcccctaa aggctggctc agcgttatgc cattttcatg
     6781 cccgaccaca ccgccagaca gctgccagtt aacgtcatgt tgatcgcggt cgtagttata
     6841 cccgaccccc agcgtaccgt aagcggcctg ccagtttgcc gtggcgctgc ccgtataccc
     6901 attattcgag gtatcaccct ggcttacgtg ataactcagg ttgtggcctt ccagtaaggt
     6961 cccacctaca cctgccagcc agctattttg cccgttgctg ttacggttgg cgttaaagga
     7021 ggcataagcg cggtcgagcg cattttcccg ggtgtagcga cgtttggtca gaacattgaa
     7081 aggcactgaa acattaagtc cgacaatacg ttcgttatcg gggatcccta cagattcatt
     7141 ccacgaaaat gagagtgaat aactgatgcc aacccagctg ctggtatacc ccacctgata
     7201 ccaggtatct gaatccgaag tattccagta tttttgatgg gtaccagaga tataaagcga
     7261 gccaaagtca ttaagtgatt gtgaaatatt taactgcaaa cggtctttac ggctaaagcg
     7321 taaattgtgg taattcacga ttatcggttc atcgcgatgc tcaccgtcgt aatcgtaatc
     7381 atattcgtac ccctccattc gacgatacgc aacatcatca agggtataaa aaccttgtgt
     7441 cgaatagcgg taacccatta actgaaaatt ggtgccgaag gtgttcatcg atttcgcata
     7501 gaggaagcga atggaatccc cctcatgacg actgtcgtcg gctaactgac tgcgcgcatg
     7561 ggtcacatcc agcgacactg tgccccagtt cccgaggttg cgccccagcc ctaataaaaa
     7621 ggcggtgtaa ttggcagata attgcgtccc gccgtaggca gtaaattcct gtggtaaacc
     7681 gccgagtgcc gtaccctgaa aaaagaaagg cgatgattgc tgactattac cgctacgaaa
     7741 atcgcccgcc gtcaggtcaa atttgaaacg cccttcgcgt tgtaaaattg gcactgttga
     7801 atacggaatt gtgtaattct gctgattacc atcgcgctcg tcgatggtga cgtccagatc
     7861 gccatttgaa gatgtcgggt gcaaatctgt aatttcaaaa gcgccggggg aaacatagct
     7921 ttgatagata ataaaaccat tttgccgaat cgttagctgg gccgccgtac gggcaatccc
     7981 acgtactgtt ggggcaaacc cttgctggct atcaggatac atattatcgg aagagtaaag
     8041 ccgtatacca cgaaatccaa caccatcgaa aatatcactt cctgtattgc cgtctcccat
     8101 taccagttca cttttcagcg gaataatggc gcgctgtacc caggtgccaa tattattcca
     8161 ctgttctgaa tgatatccat ttccgcgaaa atagttccag gaaccattgt tgcgtaaacg
     8221 ccacgggcca atattaatcc cgctgagctc actaaaaaaa tagctatcgt taccattacc
     8281 tctgttaccg gtgaaattat aattcaggag taaagcagga ataccttcat cccactcttc
     8341 aggcggaatg taaccgtgag cgctactcaa caacgctatc tgaggaatag tgatgttcag
     8401 gcgcatcgct gcaaaatcaa agttaattgt ggcatcaggt acagctttca gtaaattgat
     8461 gcatttattg ttttgctgct gggctaactc gggaaatgca ctgctattaa ggctaattcg
     8521 ttcaaggagt acctggttaa aacagggcat taacccacct gatttatcac ctgtattttc
     8581 tgttgtcgat tcaaatacga tatcctgtga accaatgaac tcatcattac gccagagatc
     8641 tacccgatac acgcccgcag gttgatgatt acctctctca aagcgcgata aatcagcaac
     8701 gaatgtgcca ttctctaata aaaatgccgg gttgaaataa ctttccgcat agcttaaaaa
     8761 ggagggtaaa atggcaagag atgaataaca aattccggcc agacaccagg tatacctctg
     8821 cggaatatcc gtagtagtgg gtattttcat aattagctct gatattgtca gatgattcgg
     8881 ttagtttaag ttcacttctc tgaccggggt tactgaacca taatcattaa cggtctgaaa
     8941 tttcagcttt ccctgcactc ctgagggtaa gggaatttgt gaattaactc tgggtgcagc
     9001 catagcattc cccaactttt ggttgcccac ttgtaagtta accagagtga cataaaaagg
     9061 tgagggatta tgaacattga tatagttacc ggatcgcgaa aacttgagtg tgtccggtgc
     9121 ttctgcgggt aattcttgta attgaattag acggagaaat aatttcatgc gcgataaaat
     9181 cgccagttgc aaaacattcc tgccgttcaa tgcattttta tctaccgaag ggatcgtctt
     9241 aacattcatc cagaacagag actcacgatc tgctgccagc ggtggaccgg tgtaaataat
     9301 acgcaaggta ttttcgcttt tgggttcact aacaaacagt ggcggtgtaa tgatgaatga
     9361 cttttctttt acaccgctgc tgttttcaat ccacgaattg accagaaagc gctcattggt
     9421 atggctattt ctaatccata ccgcagtctg tttagcatca gcgggataaa taatacgcgt
     9481 ggcacctaat gcgatccctc cggcagcatg ggcgctggcc gaaatgatta aataaaatat
     9541 aatgagcatc aataacttta ttttagtcat catagttaat acgccttgtg tattcctgac
     9601 ggcaaaaata agaaatataa agaaatgatt gctttggttt cttacaacac atcattccct
     9661 tttcatttaa taaagcggca tgatacaaag tgaacatgat ttcgatttac attgccacca
     9721 taccgcttca gacaaccggt acattacttt gttataagac gtgatatata attcaaaaca
     9781 acgtggtttt gattattcgt atctcataat aaaagtcgcg tcagcatttg cttgccctgc
     9841 tgatgcactt gtaccggtgc ctttataacg tgcagaaaaa tgaagaacgt tggtcccggg
     9901 gatcaagttc tggttggttg agaagctatt cccatcaggt ttgagaattg cggatgtatt
     9961 atcaagtatt tcaataccga cacccgttgc tgttgttgta ttggtactgg atgcaatggc
    10021 caataaatta tcattgattg catcagcctg accagaaaat gcaacggcag cattagcggc
    10081 aacaacagga tcgcagtcgt tcaactgaat ggtgaatgga attaatgcag aggtattacc
    10141 aacagcattg aaaatatcgg tacgatattg accgagtgtg acaacctggt ctgccgagtc
    10201 agtattcact gaacaggcag cattcaccag ttcgccttca aagtgaattg taccgccatc
    10261 tacaacggca gcataactac ccgtagcggc gaatagtgcg gcagccagcg cagacgaaat
    10321 aaatcttaat ttcatatata ttccttcaat ctcatttatc gactccacat ccgtatataa
    10381 ccgattactt tatttaagac actgatagta gtaaaattcc tttttatcct ctaagaatgt
    10441 cttaattgaa aatatgcact ctattctaaa aaatagagag ccccgttaga tgaatacttt
    10501 cgcgcaaaat atattcaaca caaatataga cctgaagcgg taaattacca ggctgaaaat
    10561 tctttttata ttgtcaggta tttcttaaat tatcttaatc cttaaacaag gaaataaatc
    10621 agttccagat ttacaacgcc atcatggacg aaaaatgaag ctttcaatct cagcgacctc
    10681 agcgacggtg cgcctcacct tcgcaagagg tcgcttcacg cgataaatct gaaacgaaac
    10741 ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta cgtaacagat
    10801 ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc gcagcaggtg
    10861 cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg ggcaccagga
    10921 ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc aagcaaacgc
    10981 aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga aaccaccagc
    11041 gaagcggagc tgttggagct tatcgatgcg ctaaatgccg acaacaccat cgatggcatt
    11101 ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga acgtattcat
    11161 ccggacaaag acgtggacgg tttccatcct tataacgtcg gtcgtctgtg ccagcgcgcg
    11221 ccgcgtctgc gtccctgcac cccgcgcggt atcgtcacgc tgcttgagcg ttacaacatt
    11281 gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg tcgtccaatg
    11341 agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgttt cactaaaaat
    11401 ctgcgtcacc acgtagaaaa tgccgatctg ttgatcgttg ccgtcggcaa gccgggcttt
    11461 attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat caaccgtctg
    11521 gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg cgcctcatac
    11581 attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat tgaaaacacg
    11641 cttcaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga cattttcttt
    11701 aggtaaacat ccgcacgttg agctgtgcga tttgctgaaa ctggaaggct ggagcgaaag
    11761 cggcgcgcag gcgaaaatcg cgatttccga aggccaggtg aaagtcgacg gtgcggttga
    11821 aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag gtcacagcgt
    11881 acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct cttttcagac
    11941 ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc cgaagcgacc
    12001 atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt agggttgggt
    12061 tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat gctgcccgac
    12121 gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg tcatcgcggg
    12181 tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgtt tattggacgc
    12241 cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc gttgctatcg
    12301 cacagcttgc tggattcggt aaccactggc ggtaaaggcg ttggctggct gtggccgtgg
    12361 tcagatgaac gctttttcgc tccctggcag gtgataaaag tcgcgccgtt tgcattgtct
    12421 cgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg ggtgtggttg
    12481 ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga tgcgaaaact
    12541 cgcatccggc aatagcgcaa ttacttacga cgccagatgg tcccttgcgg gccatcttcc
    12601 agcacgatcc ccatctcgtt aagacgatca cgcgccgcat ccgccgccgc ccagtctttc
    12661 gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg
    12721 tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag gcccaataca
    12781 gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc ttctgctttc
    12841 agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg ggtgttgaaa
    12901 tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg
    12961 gttttatctg tgccgcgcag cgcagtgtag agacgctcca gcgccgcacg cgcctgcttc
    13021 aggttctctt cgctgtagtt caactggctg cgatagtggc ccgacatcag gaagtaacgc
    13081 acggtttccg cgtcgtagta tttcagcaca tcgcgcacgg taaagaagtt acccagcgat
    13141 ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta gttcacatac
    13201 tgaccatcat gggcacaggt ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg
    13261 tctgaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt catggccgaa
    13321 cattcaatgt gccagccagg acgacccgcg ccccacggag acggccagct cggttcgccc
    13381 tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcatc aaccacgtca
    13441 acgcgcgcgc cagcctgcag ctggtcgaga tcctgacgcg acagcacgcc ataagttgga
    13501 tcggtcggga cgtcaaacat cacgtcgccg ttgtccgcca cataagcgtg acctttggcg
    13561 atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg cggctccata
    13621 tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat catgcgatcc
    13681 accagcgcca caaagctttc gccattttca ttggcgcgtt tgatgatttt gtcgtcgata
    13741 tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata gcgcgcaacc
    13801 acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta aacggtgatt
    13861 ccacacacgt acatgccgac ttccccggcg tgaataggct taaattcctc tttttggcgt
    13921 gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt gggtaattga
    13981 gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga tgatccgatc
    14041 gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa gcaccaatca
    14101 aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata ttgtcatcaa
    14161 aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact gccgcgaagg
    14221 tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc agggcggcgg
    14281 ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg aagccaacaa
    14341 cggcctgaaa aatacccgtg gtacgctggc aatggcacgt actcaggctc cgcactctgc
    14401 aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct ctggcgaaag
    14461 cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gacggcatgg acgtggtaga
    14521 caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc caaaagaaga
    14581 cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt tattgcagat
    14641 cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt tttagcgggg
    14701 gaagcccgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc atggattggc
    14761 gacgacgatc ccaacccact ccatcgcaag atggcggcgg cgatcaaagc ggtgtccgat
    14821 tccggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg caaacgcttt
    14881 gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact ttatggtcgc
    14941 cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta tcaggctttt
    15001 cgcgccaagg tccacaaacc ctggctgcag acgctattcc tcgccctgcc gttgtttgtg
    15061 cgcaaacgca ttgccgcgcg aatgcgcgcg aacagcaaag aagccaacag cagtaaatcg
    15121 ctggcgatca tggacgttaa ccaaaacgcg gtggtcagtg cgatggaaaa acatcaggtg
    15181 caatggctga tccacgggca tacccatcgc ccggcggtgc atgaacttat cgccaatcag
    15241 caacctgctt ttcgcgtggt actgggtgcc tggcatacgg aaggttcaat ggtgaaagtc
    15301 acggcggatg acgttgagct gattcatttt ccgttttaaa aaacccgcaa ctttgctgat
    15361 ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg tgccctctaa
    15421 agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa taatccggcg
    15481 cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt cgccgccgaa
    15541 atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca ccgcaccccc
    15601 gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt gattattgcg
    15661 ggcgcaggcg gcgcagcgca tctgccaggc atgattgccg ccaaaacgct ggtgccggtg
    15721 ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta ctccatcgta
    15781 caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac
    15841 gcggcgttac tggcagcaca aattcttgcg actcatgata aagaactgca ccagcgtctg
    15901 aatgactggc gcaaagccca gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg
    15961 gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag
    16021 gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg gcggcggtgc
    16081 cttttcaaca aagcgtgatt accgctgaga tagaacgctg gccggaaacc gcattaaccc
    16141 gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt attgctgacc
    16201 gtctgactca gaagcagctt ttcgataagc tccacctgcc gactgcaccg tggcagttac
    16261 ttgccgaacg cagcgagtgg cctgcggtgt ttgatcgttt aggtgagctg gcgattgtta
    16321 agcgtcgcac tggtggttat gacggtcgcg gtcaatggcg tttacgcgca aatgaaaccg
    16381 aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt aacttctctg
    16441 gtgaagtgtc gctggttggc gcgcacggct ttgatggcag caccgtgttt tatccgctga
    16501 cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg caggccaacg
    16561 cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag ctgggctatg
    16621 tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc aacgaactgg
    16681 ctccgcgtgt gcataacagc ggtcactgga cacaaaacgg tgccagcatc agccagtttg
    16741 agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtggtg aataatccgt
    16801 cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa ctgccgctgg
    16861 tgcatctgca ctggtacgac aaagaagtcc gtccgggccg taaagtgggg catctgaatt
    16921 tgaccgacag cgacacatcg cgtctgtccg cgacgctgga agccttaatc ccgctgctgc
    16981 cgccggaata tgccagcggc gtgatgtggg cacagagtaa gttcagttaa cctggtgctc
    17041 atttgattgc cggatgcggc gtgaacgcct tatccggcct acaccgatcc ggtacccatt
    17101 gtaggcctga taagacgcgt caagcgtcgc atcaggcatg gtgcaccgat tgctggatgc
    17161 tctcatcact acagcgaaat acaggtcccc gcttcgcccg ccagcgtctc ttcaattcgc
    17221 gataacgccc caatccacgc gggtttacca cggcttctga cataacaact caccgccgtt
    17281 accttcggcc ccatcgaacc atcggctttg gcaaatggcg ctaactcatc cggtgtggca
    17341 tggcgaatgg cgcgttgctg cggcgttccc cagttttcat ataccgcatc agcatcagtg
    17401 aggatcacca gcccatcggc gttaatctgc tcagcgagca acgcagcagc gagatcttta
    17461 tcaatcaccg cttcactccc tgccccatct tccgtcacag gcacaccgcc gccgccactg
    17521 caaatcacca catgcccctc tttgagcaac aactcgatgg cttcgctgtc gagaatttta
    17581 cgcggttgcg gagacgccac cacccggcgc aaatatttac catcacgttt catctgccag
    17641 ccgtaagctg cttccagtgc ctcttgttct tctggctgat agaccggacc gataaacttc
    17701 tctggctgca aaaacgccgg atcatcaggc gaaacttcaa tgcgcgtcag caccgtcgtg
    17761 acaggcggca tctgcggctg tgcgctcaaa ctctgcgcca gcatatagcc aatcatcccc
    17821 tgactttctg ccaccagcac atccagcgga tacggttcca cttctttcca cgccagattc
    17881 tgtaacgcca gcagccccac ctgcggcccg ttgccgtgaa caatcgccag ccgataagaa
    17941 cgggccaggc gcgccagcgc aggtacagca ctggcgatat tgcgatattg attttctgcc
    18001 gtcagcgcct caccgcgctg aagcaaggca ttgcccccaa gagctacaac cagtgttttc
    18061 atggttttcc ttgtaataat tgttgaccaa gccagaaccc cagcagtgta tctgcccctg
    18121 acgtatgccc cagcgccagc agcgaatcaa tcgcaacagc ggtacgtttc gggcaactca
    18181 gagcatggac aaagtgcagg agtggcgagg cgaaatatcc ttgcgcggca taacgtaaat
    18241 aactgacgct caccgctgtg gtaacgagtt gaagattgtc ggaacaggca aaaaactggc
    18301 gaccggagcg cgcatctaaa gcaccataat accaggccgc cagcagcatt ccgctcagcg
    18361 tgtcatcatg acttggcgtt aatccggggc ctttacccag ccagtgccgc cagtcggtct
    18421 taacgccatt gagcgcggcc tgaaaacagt gacgaaactg gcgtaactca gcaggcagcg
    18481 gatcgcctgc cgccaacgcc agtggcccga aaagcccggt ttcctccgcg cgttgcatcc
    18541 atgcagctgc aagcggttga ggatgcgcag gcggcgtaat acgcagcaaa caataacgct
    18601 gtggctgttt aaccgtgaaa cgcccgaggc gaatcccttg agccacaacc tgtggtcgtt
    18661 cattgccgca taatccaccg cataacgcat cgaattgcgc gcggcgaagc acccatcctc
    18721 cggggccgaa accactaccc tgacgatgca gcgttaacag ttcgccgctt tccgtcatca
    18781 ggttaatcgc ccgccgccac actcccgcta accgccagga ctggcgataa ttcggtgcgc
    18841 tactactggc aagcagagga tggatgatcg tcattgcgcg ttcctcaacc aatccccatg
    18901 ctttcagcca gtgcttccag cgcctgttca aaacacgcca gcggtgcccg cacagtgcct
    18961 gcgccaatct gcccgatccc cgcctcttta tgggcgatac cggtattgat gagtggcgta
    19021 atgccggttt ctaccacgcg acgaatgtcc agtcccaggc acgcgccctg aaaatcccag
    19081 cctgggatct gcaactgcat attgcgctca aggtaaattt ccgccatctc ttcagatacc
    19141 gctctggccg cttccatgcc acccgcaccg acaaagcgcg ttacgccagg cgctgcgatc
    19201 attgccgcac ctccgatacc aaaggtttcg gtaatcgcgc tatcgcccat atccgggttc
    19261 gcctgctcct gcgagaagcc ggtgaaaaac agaccttgcg gagtgtttac aggcgcagta
    19321 aaccagcgtt cgcccagccc gcttacccga atcccgaaca tattgccgtt gcgggtcatt
    19381 gccgtgacga tgctgcctgc gcggatcatc gcgccagcat ccatcgccgc cttgcagtaa
    19441 gccatcgcga ggttgaggaa gaactgatcg gtcacgctga ggaaatccat cacttcggcg
    19501 atgtgctgtt tatcatgatc gaggcgagca atttgtgggg ccagcgcacg cattaacagt
    19561 gcagaggaag caatattgcg ttgatggaac tcatcgccca tcgtaatgcc ctgcgccatc
    19621 atcgccgtga gatcgatacc gcgctccatg cgccccagcg ccgcgcttaa tactggcatt
    19681 aacacatcgc gcatccagcg atggcgagtc aggacatctt cgccgtaagc gccaaaacgc
    19741 atcactttgc cgataccttc gttgaggttg cagtacgccc ggttgccgtc ggtcacgttc
    19801 tcgaccacca gcatcggcat actggcagaa gtaataccgc ccattggacc gacggcattc
    19861 acatggtgac aaggaatgaa gttcacttcc ccctgctcca gtattgccag cgcctgcgct
    19921 tcatctttca cccagccttc gaacagacat gcgcccacgc acgccccttt catgggtccg
    19981 gtcatctcct gccagcgcat tggcggcccg gcgtgaagca gtgttttgcc ctcgtttagt
    20041 tcgctgataa gtgaagaagc cggttgcaca tccagccagt gtggacgagc gcgacgaatt
    20101 tgttcgataa ccgcagcatt ggcttgcgct actgatgtaa acatgggaac cccttattgc
    20161 aaacgttcta ataaacgagc cagtttttta ttgccgccgg cgactggcga ccattggtaa
    20221 tgcacaaccg gtttgctggc gctttgtagc tccagcgcaa agctgcgtaa tccgatgtta
    20281 atcacggcga cgttttccag taatgacggt gtgtgttgct gagtggcagg cgaaagcgga
    20341 cgaatcagtg ccgccgccag caaggtggct tccggcagcg aactcactac cgcgatccct
    20401 gcatcttcca gcgtggcgat ttgctgcgag cggcattgcg ggtcacgttc agtgcctgtc
    20461 accgtggcaa tggcatacag tggttgattg tctgaacgcg cggcacatgc tttttgccag
    20521 gcgcttacca gcgaggcggc aggatcggcg gtcgcaccga agccaatcac gacatcaagc
    20581 agcaatacgc gcacttgcgg tttagcgccg agatcggcaa ttaactggtt gcgtaaggct
    20641 gggtcgatca tcggatgggg acgcccgacg gtgtagaaat catcgccgag gtcaataatc
    20701 tggtgaccat cggcgtcgag catcatgccg tgatgatggg tatcgtcggc ttccacgcca
    20761 aggtgtccag caagtaatcc cgccgcttcg gcagccagcg taccgccggt atacaaaccg
    20821 caaataaatc cgctgctgac aggcgctatt gcgttacgtc gcgccgtgac acgtgaaagc
    20881 aggcaagcca ggcgtgcggc ctcatccagc gaggaggcaa accagacatt ctcgtcgcgg
    20941 gccaccgccg gggtataacc taaaaacagc gccaccgtcg gtttgccggt tgctttcatg
    21001 gcattaacaa ttttcagacg cacagcttcg gcaggcggtt ttgaaacaaa tgccagcact
    21061 tcgcttttct cgtctgcact gagcatttcc agcgccgtca gcgcactgat gccgcctact
    21121 tcacggctga ggtcgcgccc gccaaggcca atcgcgtgag taattccctc ccctgccagc
    21181 gcaatctgcg agcacagctc ctgaatcccg gtaccggaag cgccaatgac gccaatgttg
    21241 ccttccggca tcacgttagc aaaagccagc ggcgtgccgg caatcatcga tgtaccgcag
    21301 tccggcccca tcaccagcaa gcctttttcc cgcgcgcggg ttttaagttg gatttcatct
    21361 tccagcgtga cgttatcgga gaacatcatc acgttgaggt tgcgatcaag cgcctggttc
    21421 gccagctccg ccgcatactc accagccact gaaatcagcg ccagactggc gtcgggtaat
    21481 ttctgacagg cactgtccca gcgacgcacc tgcgtcaacg cctggctgct gcctgccccc
    21541 tgcgccagtt gttttagcgc ctcttcaagc tgctgcataa tcgcctgcgc gatccccgca
    21601 tccgccgctt cgctacgaat tgccacgcaa atatcgttcg gcgtggcgtt attaaaatcg
    21661 tcatgccaga aacctgtggt atctaataac gctttattgg cgggcgtacc catcattacg
    21721 gaaacatcat caacattttc tgattcgctg agttttcgtg aaataatcat taaactgacc
    21781 gaatcctgaa aacacccttt tttaataaag gcgtggatca taacaactcc ttaaaaattg
    21841 cacccatggc aattacacga gcagtgttaa tgtggcgatt acggtatcga tgacaacggg
    21901 taaataaggt gatgaatatc acacagggat tattaacaaa agaattcaaa taaccgccat
    21961 gctattacgt gttatttttt aataagcgtg gttgttttaa aaaccaggtt aaaagcgagc
    22021 ggattatcaa ataaattcta actatatttt tttgcctgtc tggatcacat aatccagata
    22081 ttttccctgt atgttaattg cagtcatgct tcacaccgtc gttaaaaagg aagacagatg
    22141 aaaatcagtc gggaaacact ccaccagcta attgagaata aactctgcca ggctgggtta
    22201 aaacgtgagc acgctgcaac cgtggctgaa gtattggttt acgccgatgc cagagggatc
    22261 cactctcatg gcgcggtgcg cgtggaatac tacgcggaac gcatttcaaa aggcggcacc
    22321 aaccgcgaac cggagtttcg tcttgaggaa accgggccgt gctcggcaat tttacatgcc
    22381 gacaatgccg ccggacaggt cgcggcgaaa atgggtatgg aacatgccat caaaaccgcc
    22441 cagcaaaatg gcgttgcggt ggtcggtatc agccggatgg gtcacagcgg cgcaatctct
    22501 tattttgtgc agcaggcagc ccgcgccgga ttaattggca tttcgatgtg ccagtccgat
    22561 ccaatggt
//
LOCUS       DACAPB010000062        22392 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.062, whole genome shotgun sequence.
ACCESSION   DACAPB010000062 DACAPB010000000
VERSION     DACAPB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.062"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1186
                     /locus_tag="IT904_002141"
     CDS             <1..1186
                     /locus_tag="IT904_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191918.1"
                     /translation="APSTPQPPLPKDVTCELERTAISLPAELTLNRFNPNGLAQSQVL
                     HRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAALIEAACFGATLQEAARHK
                     LEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLIAQENQFAEMGQALEVLY
                     ALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEKEFHAHLHSWQALCHILR
                     DLHSGVNLPGVFLSAAVALLERRSQAIHVPALDRGAALGALMRLEHPNASAEAALTML
                     AQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLSDDDFINALPDLRAAMAW
                     LPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAYHQQLEQQALASLQHWGVF
                     HV"
     gene            1179..2315
                     /locus_tag="IT904_002142"
                     /pseudo
     CDS             1179..2315
                     /locus_tag="IT904_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            2312..4312
                     /locus_tag="IT904_002143"
     CDS             2312..4312
                     /locus_tag="IT904_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAP3191919.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPAEKEE
                     EWDLAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHIVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGAPAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTEDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLIRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLGQFRNTESEARVEAMNNVVLLCEQLFPMLQQQG"
     gene            4437..4898
                     /locus_tag="IT904_002144"
     CDS             4437..4898
                     /locus_tag="IT904_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAP3191920.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIPGVEEKLTYTDTYA
                     QENVTIDMEKVDFKSLQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(4939..5409)
                     /locus_tag="IT904_002145"
     CDS             complement(4939..5409)
                     /locus_tag="IT904_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP3191921.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(5456..6175)
                     /gene="yehT"
                     /locus_tag="IT904_002146"
     CDS             complement(5456..6175)
                     /gene="yehT"
                     /locus_tag="IT904_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAP3191922.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(6172..7857)
                     /gene="btsS"
                     /locus_tag="IT904_002147"
     CDS             complement(6172..7857)
                     /gene="btsS"
                     /locus_tag="IT904_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAP3191923.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            8079..8810
                     /gene="mlrA"
                     /locus_tag="IT904_002148"
     CDS             8079..8810
                     /gene="mlrA"
                     /locus_tag="IT904_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAP3191924.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            8870..8977
                     /locus_tag="IT904_002149"
     CDS             8870..8977
                     /locus_tag="IT904_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAP3191925.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(8958..9689)
                     /gene="yehW"
                     /locus_tag="IT904_002150"
     CDS             complement(8958..9689)
                     /gene="yehW"
                     /locus_tag="IT904_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAP3191926.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLP
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(9694..10620)
                     /gene="yehX"
                     /locus_tag="IT904_002151"
     CDS             complement(9694..10620)
                     /gene="yehX"
                     /locus_tag="IT904_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAP3191927.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNMQGQPCGTLHFQDLLVEA"
     gene            complement(10613..11770)
                     /gene="yehY"
                     /locus_tag="IT904_002152"
     CDS             complement(10613..11770)
                     /gene="yehY"
                     /locus_tag="IT904_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAP3191928.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGARRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(11777..12694)
                     /gene="osmF"
                     /locus_tag="IT904_002153"
     CDS             complement(11777..12694)
                     /gene="osmF"
                     /locus_tag="IT904_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAP3191929.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(12946..15243)
                     /gene="bglX"
                     /locus_tag="IT904_002154"
     CDS             complement(12946..15243)
                     /gene="bglX"
                     /locus_tag="IT904_002154"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAP3191930.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVNELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     PEPGKFNVFIGTDSARVKKGEFELL"
     gene            15439..17154
                     /gene="dld"
                     /locus_tag="IT904_002155"
     CDS             15439..17154
                     /gene="dld"
                     /locus_tag="IT904_002155"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP3191931.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFNQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(17193..18125)
                     /gene="pbpG"
                     /locus_tag="IT904_002156"
     CDS             complement(17193..18125)
                     /gene="pbpG"
                     /locus_tag="IT904_002156"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAP3191932.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(18299..18886)
                     /locus_tag="IT904_002157"
     CDS             complement(18299..18886)
                     /locus_tag="IT904_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAP3191933.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFVGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            19056..19634
                     /locus_tag="IT904_002158"
     CDS             19056..19634
                     /locus_tag="IT904_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP3191934.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(19764..20525)
                     /locus_tag="IT904_002159"
     CDS             complement(19764..20525)
                     /locus_tag="IT904_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3191935.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIATRQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(20578..22104)
                     /gene="mdtQ"
                     /locus_tag="IT904_002160"
     CDS             complement(20578..22104)
                     /gene="mdtQ"
                     /locus_tag="IT904_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAP3191936.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLSALTNHQTKALKLNPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            22238..22321
                     /gene="yohP"
                     /locus_tag="IT904_002161"
     CDS             22238..22321
                     /gene="yohP"
                     /locus_tag="IT904_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAP3191937.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         5384 a         6115 c         5682 g         5211 t
ORIGIN      
        1 tgccccttct acaccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac
       61 cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg gactagcgca
      121 aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac gccagcaggg
      181 aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc gcccgcttag
      241 ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactccagg aagccgcacg
      301 ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagtatca ccacatgtct
      361 tagccaggcg gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac
      421 attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata
      481 tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac
      541 gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tctaacggca gaccagatga
      601 aaaggagttt cacgctcacc tgcatagctg gcaagcgctt tgccatatcc tgcgcgatct
      661 acatagcggc gttaatttac ccggcgtttt tctttctgcg gcggtagcct tactggagcg
      721 ccgcagtcag gcgattcatg tcccggcgct ggatcgcggc gcggctcttg gcgcactgat
      781 gcgtctggaa catcccaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc
      841 cccggcacaa tccggcgagg cgctgcacgg tttgctggcg ctcgcccgtc atcaactggc
      901 ctgccaacca gcatttatcg ccggtttcag tagtcattta aatcaactga gtgacgacga
      961 ttttattaac gccctgcccg atttgcgcgc ggcgatggcc tggctaccgc cacgagaacg
     1021 cgggacactg gcgcatcagg tgcttgaaca ttatcaactg gcgcaacttc ccgtttcggc
     1081 actgcaaatg ccgttgcatt gtccaccaca agccattgca tatcatcaac aactcgaaca
     1141 gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg aacgatcttc
     1201 tgaccacccg tgagctacaa cgctggtgat taattcttgg cgaagcggca gaaacgacac
     1261 tttgtgggct ggatggcaat gcccggcaga tagatcacgc gttggagtgg ctgtatgggc
     1321 gcgatcctga acggctccag cgtggtgaac gctccggtgg attaggtggc tcaaatctca
     1381 ccacccctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg attgagcggc
     1441 tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacgaat ctcgacgtgc
     1501 tggaacgtat gcagccttct gaaagcctgc tacgcgccgt tttgcacacc aaacatctga
     1561 tgaatcccga agtactggct gccgcccgcc agatagtgcg ccaggttgtt gaagaaatta
     1621 tggctcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc
     1681 gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc
     1741 aacactggca cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca
     1801 ttaaacgcca aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggctcgatgg
     1861 tcgattcggt gatccactct gcggtgatgg cggcctgttt gtggcagtta cccggcattc
     1921 gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac gttgccgatc
     1981 cggtagaact attaatgaaa gtacaactgg gcggcggaac caatatcgcc agtgccgtgg
     2041 agtatggtcg gcaacttatt gaacaaccag caaaaagcgt cattatcctc gtgagcgatt
     2101 tttatgaagg gggttcatca tcattgctga cgcatcaggt gaaaaagtgt gtccagagcg
     2161 gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacgccttgc tatgaccgcg
     2221 atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgacg ccgggcgagc
     2281 tggcatcatg gcttgcagag aaccttcagt catgaattca ctacgtccgg aattattaga
     2341 actgacacca caggcactga cggcgttaag caatgccggt tttgttaagc gcagccttaa
     2401 ggaactggaa aatggcaacg tcccggaaat cagccatgag aacggcgctt taatcgccac
     2461 cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag aggctcagtg
     2521 cacctgtggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg
     2581 actttgtgct accgcccagc ctgcggaaaa agaagaagag tgggatctgg caatctggct
     2641 ggaagaactg gctaccctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa
     2701 aggcatcacc attgagttgt tctgtacgcc gggcgaaatt ccctctgccc gcttaccgat
     2761 gagcgatgtg cgtttttatt cccgcagcag cattcgtttc gcccgttgtg attgtattga
     2821 aggcactctt tgcgaacata tcgtactggc ggtacaggcc ttcgtcgagg ccaaagcgca
     2881 gcaagcagaa tttaaccatt taatctggca gatgcgcagc gaacacgtca catcatctga
     2941 cgatccgttt gccagcgaag aaggccaaac gtgtcgtcaa tatgttcagc aattaagcca
     3001 ggcattatgg cttggcggca tcagccagcc gcttattcac tacgaggccg ctttcagccg
     3061 cgcgcagcaa gcggcggaac gctgcaactg gcgatgggtg agtgaatcgc tacggcaact
     3121 gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg gagaatgctt
     3181 acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg cccggcgcga
     3241 cagtattggt gaagttcctc ccatgccgtg gcgcacggtc gttggcgcag gcattgccgg
     3301 agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat
     3361 tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcacct
     3421 ttcgcgcagt tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt
     3481 tagttttcag gctggcgcac cggcgggtgg gcaaattgtt tcacaagcag caaaacgcag
     3541 tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc
     3601 gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg
     3661 ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg tcgataactt
     3721 atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc gccagacgct
     3781 ggatgcgcag gtcattagcg gcgaaggtga agataatctg ctgacgttat cactgccagc
     3841 ctcagccagc gcgccttatg ccgttgaacg catggcggcg cttttgcaac aaacagagga
     3901 ccccgtgtgt ctggtttctg gctttgtcag ttttgtagag gggcaattga cactggaacc
     3961 acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gctgaaactg cgcctgtggc
     4021 accgctacct tctgccagcg ttttgcctgt gccgtctact gctcatcagt tgctgatacg
     4081 ctgccaggcg ttacttattc aactgctcca taacggctgg cgctatcagg aacaaagtgc
     4141 tattggtcag gcagagttgc tggcgaatga cctcaccgcc gtgggttttt atcggctggc
     4201 acatgtgttg ggacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa
     4261 cgttgttttg ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc
     4321 tttttactaa gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc
     4381 tatatagccc ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga
     4441 aggctttcaa taagctgttt tcccttgttg ttgcatctgt tctggttttc tctcttgctg
     4501 gctgcggtga caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg
     4561 ccattaccta tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc
     4621 aatttgcctc cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa
     4681 gcgccaaata caaaaacatc ccgggtgttg aagaaaaatt aacctatacc gatacctacg
     4741 cgcaggaaaa cgtgactatc gatatggaaa aagtggattt taaatccctg cagggtattt
     4801 caggaatcaa cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac
     4861 tggtgatgaa agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc
     4921 atggccgcca tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag
     4981 aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcacgcat
     5041 catcgctgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat
     5101 gtcatcggtt ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat
     5161 gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag
     5221 gccattgagg aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc
     5281 tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat
     5341 acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt
     5401 acttagcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca
     5461 ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat
     5521 tacgcaaaat caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga
     5581 ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta
     5641 atgtcaattc ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc
     5701 gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta tgcccagtac
     5761 aagggataaa tttcagcgcc tgttgatttt ctggtaacag cgaaacatcc tgcttgctgc
     5821 gttcctgccg caaacgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca
     5881 gataatcaaa ggcgtgttct tcaaaggctt taatggcgta ttcgtcaaac gcggtgagaa
     5941 aaacaatata cggacgatgt tccgggtcga gcatccccac catttccaga ccactgatgc
     6001 gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc
     6061 cttccacggc gtttgaacac tcaccaacga tttcaatatc gctctgctcc tgcaaaaata
     6121 cgcgcaggtt ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc
     6181 gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct
     6241 tatcccatag tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag
     6301 cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcattaa
     6361 atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt
     6421 cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg
     6481 ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc
     6541 aatttgcaga taagcattca catgttcaat ttcgtcggcg agagtaacaa actccgaagg
     6601 ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc
     6661 gctgtcgcga cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatggggatt
     6721 cacctgggcg tgaagcagtt tgatctctga ctgggtgagc atcgcttttt gccgctcata
     6781 ttgcccggca aggatctgcg ccgaaagcag ttgcgcaatc ccctcaccca acgtgcggtt
     6841 gattgaactg aataaacggt ttttggcttc atacaatttg atggtgccca tcacccgctg
     6901 attttcacca cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga
     6961 gcaacggtaa ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt
     7021 taaggtataa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt
     7081 aaaggctagc aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata
     7141 cagcacctgt gccactttca tgctgttcac ttcgttaaac ccctggcgca aaatgccttc
     7201 cgtcgaggcc gccactttca gcgcagtggc agaaaaagct gaagtgtatt tttcaaacat
     7261 cgcgcgttta tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat
     7321 cattggtgca gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc
     7381 caggatgatc aacatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg
     7441 gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag
     7501 caatccttca acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata
     7561 tcgatgtaag ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc
     7621 gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa
     7681 ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag
     7741 acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat
     7801 gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc
     7861 cgcaaaccac acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg
     7921 aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat
     7981 aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc
     8041 taaagttaaa ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg
     8101 aagtggcgtt gctttgtgat attaatcctg tcacgttacg cgcgtggcag aggcgttacg
     8161 gattgctgaa accgcaacgg acagacggcg gtcatcggct gttcaacgat gccgatattg
     8221 accggatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc aaagttaaaa
     8281 tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag caagaaacat
     8341 tactgactta cctgcaaagc ggcaatctac atagcctgcg aacgtggatc aaagagcgcg
     8401 gtcaggatta ccccgcccag acactcacca cacatctgtt tattcctctg cgccgacggc
     8461 ttcagtgcca acaaccgact ctccaggcgc tgctggcgat cctcgacggc gtactgatca
     8521 actacatcgc catttgtctg gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg
     8581 ttggctggaa tattcaggat accacccgtc tgtggctgga gggctggatt gccagtcaac
     8641 aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct gaactattcg
     8701 aaggccgtac attgctggtg tggtgcggtg aaaatcgaac ctccgcccaa cagcaacaac
     8761 tcaccagttg gcaggaacaa ggccatgata ttttcccact cggcatttaa tgattcgtta
     8821 acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta tgcgcatagc
     8881 taaaattggg gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat
     8941 gctcgcaggt tataccttta ttttgcgtgc tggctaagcg cctgcaccag cctttcaaac
     9001 aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ccccctggat cacatacgcg
     9061 gtattaaatc cgctaagccc gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt
     9121 gaggcgatcg tcgccgtacc aatgttgata atcaccgaag ttcgcacgcc cgccagaatc
     9181 accggagccg ccagcggtag ctcgacctta ggcagtcgct gaccacgact cattcccata
     9241 cctttcgcaa cttctgtcac gctggcatca atcgctccca gcccggcaag tgtcgcctgc
     9301 aggacgggca gcacaccgta aaggatcaag gcgataatcg ctggttgcag accaaagccg
     9361 atcaccggaa cggcaatcgc cagcactgcg acaggcggaa aagtctgccc aacggcggca
     9421 atagtttcca ccagtgggcg aaattccgcg ccccacgggc gagtgactgc aattccggca
     9481 ccagtgccaa tgatcactgc aaacaaactc gaaattccca ccagccagaa atgagccagt
     9541 gccagagctg caaaactttc ttgctgataa acgggtcgtg gcagttgtgg gaacaaggca
     9601 gcaaacagcg gctggctgta aggcagccag aaaatcagtg ccacaaacaa agcaatgagc
     9661 cagaacagcg gatcgcgcaa catcttcata cgcttacgcc tccaccagca gatcctgaaa
     9721 atgcagcgtg ccgcaaggct ggccctgcat gttcaccacc ggcagcacct cgcatccccg
     9781 cgcaacaaac agagagagcg catcgcgtag cgtcatctct tctgccagtg cctcaccatc
     9841 tgctcgttct tcgcgacgca cgtaatccgc cacactacgt aacgaaagca ggcgcacacc
     9901 cagttcacta cgtccaaaaa actggcggac aaaatcattc gccggacgag tcaacatcgt
     9961 cagcggattg ccctgctgca ctacttcacc gtgatccatc aataccagat gttctgccag
    10021 ccgtagcgcc tcatcaatat catgagtgac cagcacaatg gtacgcccca gcaaacggtg
    10081 aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc gggtccagtg cgccaaaagg
    10141 ttcatccatc agtaagactt gcggatcggc agccagtgca cgcgccactc ccacacgttg
    10201 ctgctgacca ccggaaagct gatgcggata acgctcacgc aaatttgact ccagccccag
    10261 tagcgccatt aattcgtcga tacgatcgtc aatccgcgcc cgcgaccatt tttgtaattg
    10321 cggcacggta gcgatgtttt gcgccacgct ccagtggggg aacaggccaa tagattgaat
    10381 ggcatagccc atccggcggc gcaactccag tactggcagc gagcgaattt cttctccggc
    10441 aaagcggatc actccgctgt catgctccac caggcggtta atcattttca gggtggtgga
    10501 tttgccggag ccagatgtgc caatcagcac cgaaaaactc ccttcctgaa aattgagatt
    10561 gagatcgtta acggcttttt gtgcgccgaa cagtttgctg acatggctaa attcaatcat
    10621 tacgtttcac cttcagcagt gcgataagca aatcgaacag cgcgtcggta agcaccgcca
    10681 gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcgctgctt agcagcccct
    10741 ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc gccataccta
    10801 cagtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc agtggtaact
    10861 gaacatgcag gaatcgccgc gccccgctca tgcccatcgc tctggcgctc tccagcacat
    10921 cgcgcgggat ctggttcaag ccgactacca cgccgcgcac cagcggcagc aaggcataga
    10981 gcaccagcgc aatcagtgcg ggtgtcattc cggttcctgc tatgccgagc ttccccagcc
    11041 acggaaaggc cgtcaccagc gcggcaagcg gcgcaatcaa caggccaaag agcgccaccg
    11101 aaggcacggt ctgaatgaca ttgagcagag aaaaaattgc cccctgccga gcagtggaaa
    11161 agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca
    11221 gcaacgtcag atgttgtgcc agcgcgtcgt caaacacatc ctgacggttg gcgtattctt
    11281 tcattagtga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga ataatggcaa
    11341 tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg atggcatcgc
    11401 tacaggccag caatgccagc gccgcagcca gccagaaacc actgccgagg ctggtacgcg
    11461 ccagcgcact gccattttgc gccagttggg tcgccgcctt tccagctccc cacaccagca
    11521 atacgaagac gaattgcgcc agaatgagtg cacaaatgct gcctttttta ccgggaataa
    11581 aacaggccgt cagccaggcg caaccaacgc ccaccagcat ccagatcgtt tgcggccaca
    11641 gctgccagag atgacgcccc tcacccgaaa ctaaacgatt aggcgcgtaa ctgataaacg
    11701 gcagcgccgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga ttaatacgga
    11761 aataagtcac gggaaattac ttcgtccacc ctttttgttt caggtagtcg gcagccactt
    11821 ttttggcatc cagtccttcc acagcaatgc tggcattcag ttgctgcaat gtttttgcat
    11881 cgaggctggc gaagactggc tgtagccact gtgccatttg cggatactcc ttcaacaccg
    11941 actcacgcac cactggtgca ggcgcgtaga taggttgcac accttgcgga tcgcttaagg
    12001 tttgcagccc cagcgccgct accgggccgt cagtgccgta agccattgca gcattaacgc
    12061 cagaggtttg ctgggcagcg gctttgatcg tcaccgccgt gtcgccacca gccagtgaca
    12121 gcaactgatc ctgaccgagc ttaaagccat aggctttttc aaacgcgggt aacgcatcgg
    12181 cgcgttcgat aaactctgcc gaggctgcca gtttgaaggt gccgccctct tgcagataac
    12241 gactcaggtc ggcaagcgaa gtgagtttat ttttctctgc cacatcctga cgcacggcga
    12301 tagtccaggt gttatttgca ggcgcgggcg tcagccagat taacttgttg tgctccgaat
    12361 cgagcttttt gactttctcg taaccttgct gcgcgttttt ccacgctgca tcgttttcat
    12421 ctttaaagaa gaaagcgcca ttgccggtat attccggata gatatccagt tcaccggaag
    12481 taatcgcccc gcgcaccaca ggagtcgttc cgagttgcac tttattcacc gtcggtacgc
    12541 cgtggctttc cagcacctgc aaaatgatat tgccgagtag cgcaccttcg gtatcgattt
    12601 ttgaaccgac tttaacgggg gaagccgcct gtagcggcag gctcacggct gccaacataa
    12661 ccagtgaacc tgcccagagc tttaagagtg gcatgatgct ttcctcattc ttttactgtt
    12721 gttttcagcg aattaagaga aaagcatagt tgataatggc ggggttagct tgagcggaag
    12781 gattcagttg cagaatcaga taaatactta agaggcatat tcggtccggc gtttcctgcc
    12841 ggatgcggcg taaacgcctt atccggccta caaagggcga aaattcagca agttaagata
    12901 tgaacatcag gcaaagggcg caaacgtcgc gcccttacta aagcattaca gcaactcaaa
    12961 ctcgcctttc ttaacgcgtg cggaatcagt gccgataaag acattgaact tgccaggctc
    13021 ggggtcatat ttcatctgtt gattccagaa cttcagcgcc tcaatatcaa tcgggaagct
    13081 gacggtctga gtttcgcccg gcttcagggt gattttctca aagcctttca gctgcttcac
    13141 cgggcggctc atagaagccg tcacatcctg caagtacatc tgcactaccg tggccccttc
    13201 gcgcttaccg gtgttcgtca cctgcacgct ggccgtcact ttgccgtcac gcttcatggt
    13261 cggcgcagaa agtttcacat cagagacggt gaaagtggta tagctcagac catagccgaa
    13321 cggataaagc gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt tcggcttgtc
    13381 ggcattatac ggacgaccgg tgttcagatg gctgtagtac accgggatct gcccgacaga
    13441 acgcgggaag gacattggca gcttgccgga cgggttgtaa tcgccaaaca atacatcggc
    13501 aattgcatta ccgccttcag tccccgcaaa ccaggtttcc agaatcgcat cagcctgctg
    13561 atcttctttc accagcgcca gcggacgccc gttcatcagc accagcacca gcggtttacc
    13621 ggtggctttc agcgccgcaa tcaagtcacg ttggctttgc ggaatagtga tatcggtccg
    13681 gctggaggct tcgtgcgcca tcccctgcgc ttcaccgact acagccacca ccacatcaga
    13741 ttgtttcgcc gtctgcaccg cttcatcaat catctcttgc ggcgaacgcg gatcgacttt
    13801 gaccgcttct tcatactgat tcaggaaatc gataatgcct ttgtcactgg taacgttcgc
    13861 ccctttggca tacagcactt taccgttttc accgacggaa tttttaatcc cggtcagtac
    13921 ggtcacggat tgatcggcaa caccggctgc ggaccagctg cccatcacgt cacgtttact
    13981 gtccgccagt ggcccaacca ccgcaatggt ggccgatttt ttcagcggta acgtttcgag
    14041 acggtttttc agcaacacca ggctttcgcg cgccacttca cgcgcttctt tacggtgcag
    14101 gcggctttcg gcattggtat ccaccgggtc agactctttc ggccccaaat ggctgtatgg
    14161 gtcgttaaac aaccccatat catatttaac gttcagtaca tggcgggcag catcgtccag
    14221 ctctgccatg gtcactttgc cggatttaat caacccaggc agatacttcg agtagtactc
    14281 gtcgctcata ctcatgttga ttccggattt cagcgccacg cgcaccgcat cttccgggtc
    14341 tgccgccgtg ccatgtttaa tcagctcttt gattgcaccg tgatcggaaa cggtgatgcc
    14401 tttaaagccc cactggtcgc gcagaacatc tttcagcagc caggaatcgg aggtggctgg
    14461 cgtgccgttc agcgagttca gcgccaccat cactgcgccg ctgcctgcgt ccagccccgc
    14521 tttgtacggc ggcatataat cattaaacag gcgctgcgga ctcatatcga cggtgttgta
    14581 ctctttaccg ccttctaccg cgccgtatgc ggcaaagtgt ttgacgctgg tcatcaccga
    14641 gtagcgatct gccgggcttt taccctgcat cgcttccacc atggttttac ccattgttga
    14701 ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg gatcgcgcga
    14761 gacatcgacc atcggtgccc aggtcatatt caggccatca tctgccgctt cataagcaga
    14821 gacacgtccg accgttttca ctgcatcgag gttaaaagac gaggccagac cgaggctaat
    14881 cgggaacacc gtgcgctgac cgtgcagcac gtcgtaagca aagaaaagag gaattttcag
    14941 gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg ttacggtgtt
    15001 gaaaatcgcc ccaacctgac cgtctttgat catctcgcgg atcgcctctt tcgggttatc
    15061 cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca ttttcttaag
    15121 cagttcgttg acgaacgcat cccgcgcttc gggcgttaat ggatggttgc cgaataaatc
    15181 atccgccagt gctggctgca gggccagact caccgcgatt cctactgaac atagccattt
    15241 catatggatt tttcttcctc tgttgcccga cgaggcagcg aaaatggtgc aaaaaccgta
    15301 gtttgccata agcatgatgg acagagaaaa agaatgctca gtttattgtc tgaattttca
    15361 aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat attttttcgc
    15421 caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc ctttttgaat
    15481 gaacttgccc gtctggtcgg tcattcacac ctgctcaccg atcccgcaaa aacggcccgc
    15541 tatcgcaagg gcttccgttc tggtcagggc gacgcgctgg ctgtcgtttt ccctggctca
    15601 ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ccgacaaaat tattctgatg
    15661 caggctgcca atacaggcct gaccgaagga tcgacgccaa acggtaacga ctatgatcgc
    15721 gatatcgtga tcatcagcac cctgcgtctc gacaagctgc acgttcttgg caagggcgaa
    15781 caggtgctgg cctatccggg caccacgctc tattcgctgg aaaaagccct caaaccgctg
    15841 ggacgcgaac cgcactcagt gattggatca tcgtgtatag gcgcatcggt catcggcggt
    15901 atttgtaaca actccggcgg ctcgctggtg caacgtggcc cggcgtatac cgaaatgtcg
    15961 ttattcgcgc gtataaatga agacggcaaa ctgacgctgg tgaaccatct ggggattgat
    16021 ctgggcgaaa cgccggagca gatccttagc aagctggatg atgatcgcat caaagatgac
    16081 gatgtgcgtc acgatggtcg tcacgcccac gattatgact atgtccaccg cgttcgtgat
    16141 attgaagccg acacgcccgc acgttataac gccgatcctg atcggttatt tgaatcttct
    16201 ggttgcgccg ggaagctggc ggtctttgct gtacgtctag ataccttcga agcggaaaaa
    16261 aatcagcagg tgttttatat cggcaccaac cagccggaag tgctgaccga aatccgccgt
    16321 catattctgg ctaacttcga aaatctgccg gttgccgggg aatatatgca ccgggatatc
    16381 tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa gttaggcacc
    16441 gacaagatgc cgttcttctt taatctcaag ggacgcaccg atgcgatgct ggagaaagtg
    16501 aaattcttcc gtccgcattt tactgaccgt gcgatgcaaa aattcggtca cctgttcccc
    16561 agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca tctgctgtta
    16621 aaaatggcgg gcgatggcgt gggcgaagcc aaatcgtggc tggtggatta tttcaatcag
    16681 gccgaaggcg atttctttgt ctgtacgccg gaggaaggca gcaaagcgtt tttacaccgt
    16741 ttcgccgctg cgggcgcagc aattcgttat caggcggtgc attccgatga agtcgaagac
    16801 attctggcgt tggatatcgc tctgcggcgt aacgacaccg agtggtatga gcatttaccg
    16861 ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat gtgctatgtc
    16921 ttccatcagg attacatagt gaaaaaaggc gtggatgtgc atgcgttgaa agaacagatg
    16981 ctggaactgc tacagcagcg tggcgcgcaa taccctgccg agcataacgt cggtcatttg
    17041 tataaagcac cggagacgtt gcagaagttt tatcgcgaga acgatccgac caacagtatg
    17101 aatccgggga tcggtaaaac cagtaagcgg aaaaactggc aggaagtgga gtaaaaatta
    17161 cggatggcat agtatcgcca tccgtcattc acttaatcgt tctgtgccgt ctgccccgcc
    17221 gccgccattt gggcggcttt ttgtttttta tagctcaacg ctgctgctgg aacaggcatc
    17281 actttaccgg tttcaatcca ggtacgcagg cggctggcat cggcaaaatg ggtatatttg
    17341 ccaaacgcat ccatcactac cagcgccacc ggtttattat tgataaccgt acgcatcacc
    17401 agacaatggc ccgccgcatt ggtaaaaccg gttttggtta actgaatatt ccagttatcg
    17461 cgatacacca gatggttagt attgcggaac ggcagcgtat acgtcgggtt agagaaggtt
    17521 gccatatctt cccgggtagt acttaactgc ccgatcaacg gatattgttt gctggcaatg
    17581 agcagtttgg ttaagtcacg ggcagttgaa acgttatgca ccgacaatcc ggtaggttca
    17641 acaaagcgcg tgttgttcat tccgagcgat ttcgctttcg cattcattgc cttaataaag
    17701 gctttgtaac caccgggata atggtgtgca aggcttgccg ccgcgcggtt ttctgaagac
    17761 atcagcgcca gcaacagcat atctttacgg ctgatttcgc tattcagtcg tacgcgcgaa
    17821 tagacccctt tcatctccgg cgtctggctg atatccactt ttagtttttc atccagcggc
    17881 agtcgtgcat ccagcacaac catcgcggtc attaatttgc tgatagacgc aatcggacgc
    17941 accagatccg ggtggttcga atagatcact ttgttggtat tcagatcaac aatcatcgcg
    18001 ctaccggagg caatttccgg ttgtgaagcg gtggtagcgg ctgccgtttt cgcaacggcc
    18061 tgcggtgcaa aaggcacagc cagcatcagg gccaggctaa ataaagaaac tcgaaatttc
    18121 ggcatgatga gcattcagat agtggttcac gcgcacgggt tgcgcaccgc cggagtaagg
    18181 atttactgag gctagcgacg ccatcataac gagcaaaaag tgcgatcgtc aaaggagaat
    18241 cgtgaggaaa tgctgcattg cagacattta cgccagcaat gcaacgtcaa agaactttct
    18301 agaacaaacg ataaccgtag ccccatagta taacggtaag ggcgagcagc acttccagta
    18361 ccagcacgcc aatcgccagc gtcgaactgg agaagctaag gccttcctct ttgttgatat
    18421 tcaggaagct cggaatacca aggtaaagca ggtagccggt gtaaaacagc gccaccgtgc
    18481 cgaccagcgc acacaaccag accagcggat aaagcgccac cagaccgctt aaaaacagcg
    18541 gagttgcaac gtagcccacg aagaccatgc agtgcgcaag agacggacgc tgcgggtaat
    18601 tgcgcgccat ccaccagatg acccgcccca tcaccgcaac cccagccagc ataacgccgt
    18661 aaaacaagac agccagcgcc agtccggtaa accaggataa cttcaggata gtgccatcgc
    18721 caaaattcca gccaatctgt gtagtgccaa tgaaggcgca aatcaccgga atcgccgcca
    18781 tcagcaaaac gtggtgggtg taatggtgag aaatcgtttc gttttcgcga ttaatcacct
    18841 gcatttcacg atcgggatgg gaaaacagtc cccagacatg gctcataccg cccccttgtt
    18901 gtgagttcat gaacctgaca gttcaagtat aagccagctt gtgattattt tttgttcatc
    18961 catgtaattt gcaccgtctg atgtatgtcc tttttcagcg tgaatgattc acagggtgta
    19021 tgcttacagg caaccaaagg gagacagact ggcctatgga tctcaataca ctcatctcac
    19081 aatatggtta tgccgcgctg gttatcggta gcctggcgga aggtgaaacc gtgactttgc
    19141 tgggaggcgt tgcggcacat cagggactat taaagttccc gctggtggta ctttctgtgg
    19201 cgcttggcgg catgattggc gaccaggtgc tctatctgtg cgggcggcgg tttggcggca
    19261 agctgttacg ccgtttctcg aaacatcagg ataaaattga gcgggcgcag aaacttatcc
    19321 agcgccatcc gtatctgttt gtcattggta cgcgttttat gtatggcttt cgggtcattg
    19381 gtccgacgct gattggtgcc agccagctgc cgccgaaaat ctttctgccg cttaatattc
    19441 tcggcgcatt tgcctgggcg ttgattttta ccactatcgg ttacgctggt ggtcaggtga
    19501 ttgcgccgtg gttgcacact ctcgaccagc atttgaagca ctgggtctgg ttgattctgg
    19561 tcgtggttct ggtggtaggc gtgcgctggt ggctgaaacg gcgcgggaag aaaaagccgg
    19621 atcatcaggc gtaaaaccat tgccctgggt aaggcgttcg cgccgcatcc gacatccggt
    19681 gcaccatgcc tgatgcgacg cttgtgcgtc ttatcatgcc taccaaaatt ttacccattg
    19741 aaataaccac gcacctcact gcgctattct gggttgaact gcggattcgc caacataaag
    19801 ccgccatcca ctatcaacga ctgcccggtg gtgtaatttg cgccctctga gcacagccac
    19861 gctaccaggc tggcaatctc atgcgttgcg ccaaaacgcc gcagaggaat cgaaggctcc
    19921 gcgtcgggct tcacgtcgct gtcatccatg ccattcattg gcgtggcgat cgccccaggc
    19981 gcgactgcgt tcaccaaaat cttatgcctg accagctcca gcgccatcgt tttggttaac
    20041 ccgccgagcg catgtttagc ggctgtgtaa gcgctggcat ccggcagcgg cgtatgttca
    20101 tgtaccgacg taatgttgat gatgcgaccg ccctgccctt gtttcaccat ctggcgggtc
    20161 gcaatttgtg agcataaaaa tgcaccatcg acatcaacgg taaaaatctt gcgccactca
    20221 tcgaaagcca tatcaagaaa cggcgctttg gtcattgcgc ccgcattatt caccagcaca
    20281 tcaatacgcc ccagccgttg aatgagtttc tccagcgcct gcgcaccttc tggcagttta
    20341 ccgagatcca gctgcacgat ctccgcacgt acgccgtggc taactacctc acgcgcggta
    20401 tcttttgccc cttcttcatc tgagtgccag gtaatgccaa tatcaaaccc ctgctgcgcc
    20461 agtaataacg cgcactcttt gccgatcccc gaatcggagg cggtaataat cgcaacctgt
    20521 gccatcgagt tctccactta acgctgaata aacgttaagt atagaaggcg catatcatca
    20581 gcgtttgtaa cccccgccca acgcaccagt gagttgaatg gaagcatcca gccactgccc
    20641 ttgcaataac aggccattgg cccgctcacg cagcgcgggg attctggctt cgctgacgcg
    20701 ggaaccagca atgatgcccg cgttaaagcg cgcctgcgca agacctacca cacgcaaggc
    20761 atcgcgctca atttgcgcct gatgctggtt tttctccgcc agtgtctgaa cctgactggc
    20821 tgcccgcgcc acgtcattca ccgcttcaac caccgctttg ttgtagctgg cgatagacaa
    20881 gttgctttcg gcttttgcga tatcgagatt ggcgttaaga cgaccactat cgaaaatggg
    20941 tagcgtcagg cctgccgtaa cgcccatttg ctgcgcggaa tgacggaaca gatcgcttaa
    21001 gtgcaacgca tcctgttgca ggaaggccat caggttgatg tcaggataaa atgccgcttt
    21061 tgccgcatca atggtgctta gcgatgactc aacgtaccag tgcgccgcct gcaaatctgc
    21121 ccgccgggcc agtaaggagt accccagttc atcaggaagc tggcttgcca ctttcggcaa
    21181 cgcgactgga ttaagcttca atgcctttgt ctggtgattt gtaagtgcgc ttaaccgtgc
    21241 ctcaataatt ttcattttcc ccgcgacatc gttgagctgc tgccgggttt tgctggcatt
    21301 aatatcggtt tccacacctt caactgaaga agtaatcccg ttctgatata gctggcgatc
    21361 ggtcgcgata atggtgttct gctctttttc tatttgctgc aagaccgtgt ttaacgccgc
    21421 ctgggtttgc cactcccagt acaggcgggc tacgctgcca gccagcaatt ggcgggtttg
    21481 ctcgcgttcc gccgcccgtg ctttaaccgt acccaggcgg gcagtaacct ccgcccgatt
    21541 ctttccccag atatcgagat gccagcccgc cgttaagcca aaagtaccgt tggtgtacca
    21601 cgggccggtc gtacctgcgg ccggatcgtt cagagcaaac ggccccatta agccttctgc
    21661 cgacattttt tgccgctcca tatccgccga aaagtcgatc tgcggaccat cctgagtggc
    21721 aactgccttc gcctgggctt cagctagctg aatgcgctgt tcagccacct gcatatccgg
    21781 tgcgttctgt agtgcattgt taattaatga agtgagttga ttatcgtgat actccagcca
    21841 ccattggctg tctggccaac catttttcag cgccgtgggt aatgcggtgt caacttgtgc
    21901 agcgggcgtt tgctggctta acgcctggcg ggtttcatgc ataggcgcac acccggccag
    21961 catcagtaac agcggaaaac aggcgatggc tggataaaag gaatcacgat tcatggggga
    22021 ataatcaggt aagaaacggt gcgcggagat taccgtgtgt tgcgatatat tttttagttt
    22081 cgcgtggcaa tacatcagta gcaataaaac gacataccca gaaaaatata gactaagtaa
    22141 atgatatctt ccgatttatc ttaatcgttt atggataacg gcaaagggct tcgttttttc
    22201 ctatacttat tcagcactca caaataaagg aacgccaatg aaaattatac tctgggctgt
    22261 attgattatt ttcctgattg ggctactggt ggtgactggc gtatttaaga tgatatttta
    22321 aaaatcagat atggcatata agttgtcagg tcaggaacta gatattttgt gtacttgatt
    22381 tgcaaacagc tc
//
LOCUS       DACAPB010000063        22247 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.063, whole genome shotgun sequence.
ACCESSION   DACAPB010000063 DACAPB010000000
VERSION     DACAPB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.063"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(43..1263)
                     /gene="astC"
                     /locus_tag="IT904_002162"
     CDS             complement(43..1263)
                     /gene="astC"
                     /locus_tag="IT904_002162"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAP3191938.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTLNHRYGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            1709..2515
                     /gene="xthA"
                     /locus_tag="IT904_002163"
     CDS             1709..2515
                     /gene="xthA"
                     /locus_tag="IT904_002163"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP3191939.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            2682..3392
                     /locus_tag="IT904_002164"
     CDS             2682..3392
                     /locus_tag="IT904_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family membrane protein YdjX"
                     /protein_id="HAP3191940.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     FFGYSLYILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            3397..4074
                     /gene="ydjY"
                     /locus_tag="IT904_002165"
     CDS             3397..4074
                     /gene="ydjY"
                     /locus_tag="IT904_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAP3191941.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQQENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKIAE"
     gene            4089..4796
                     /locus_tag="IT904_002166"
     CDS             4089..4796
                     /locus_tag="IT904_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP3191942.1"
                     /translation="MMMMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            4796..5344
                     /locus_tag="IT904_002167"
     CDS             4796..5344
                     /locus_tag="IT904_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP3191943.1"
                     /translation="MGLPPLSKIPLILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            5354..6520
                     /locus_tag="IT904_002168"
     CDS             5354..6520
                     /locus_tag="IT904_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP3191944.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            6493..8028
                     /locus_tag="IT904_002169"
     CDS             6493..8028
                     /locus_tag="IT904_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3191945.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKWL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDIDQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPTWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFAVGIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            8028..8681
                     /locus_tag="IT904_002170"
     CDS             8028..8681
                     /locus_tag="IT904_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP3191946.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAEQFSCTGELWLNEQRIDILPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLEGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            8748..10055
                     /locus_tag="IT904_002171"
     CDS             8748..10055
                     /locus_tag="IT904_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAP3191947.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNSVATGERGPDSSK"
     gene            complement(10064..10684)
                     /locus_tag="IT904_002172"
     CDS             complement(10064..10684)
                     /locus_tag="IT904_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP3191948.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            10771..11178
                     /gene="nudG"
                     /locus_tag="IT904_002173"
     CDS             10771..11178
                     /gene="nudG"
                     /locus_tag="IT904_002173"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAP3191949.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQRDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(11144..11416)
                     /locus_tag="IT904_002174"
     CDS             complement(11144..11416)
                     /locus_tag="IT904_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAP3191950.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            11652..12995
                     /gene="gdhA"
                     /locus_tag="IT904_002175"
     CDS             11652..12995
                     /gene="gdhA"
                     /locus_tag="IT904_002175"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP3191951.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSLPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(13112..14152)
                     /locus_tag="IT904_002176"
     CDS             complement(13112..14152)
                     /locus_tag="IT904_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAP3191952.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSQMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(14280..16241)
                     /locus_tag="IT904_002177"
     CDS             complement(14280..16241)
                     /locus_tag="IT904_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP3191953.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(16246..17289)
                     /gene="selD"
                     /locus_tag="IT904_002178"
     CDS             complement(16246..17289)
                     /gene="selD"
                     /locus_tag="IT904_002178"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAP3191954.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(17406..17957)
                     /locus_tag="IT904_002179"
     CDS             complement(17406..17957)
                     /locus_tag="IT904_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAP3191955.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            18118..19974
                     /gene="sppA"
                     /locus_tag="IT904_002180"
     CDS             18118..19974
                     /gene="sppA"
                     /locus_tag="IT904_002180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP3191956.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            20141..21157
                     /gene="ansA"
                     /locus_tag="IT904_002181"
     CDS             20141..21157
                     /gene="ansA"
                     /locus_tag="IT904_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP3191957.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            21168..21809
                     /locus_tag="IT904_002182"
     CDS             21168..21809
                     /locus_tag="IT904_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyrazinamidase/nicotinamidase"
                     /protein_id="HAP3191958.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(21902..>22247)
                     /locus_tag="IT904_002183"
     CDS             complement(21902..>22247)
                     /locus_tag="IT904_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3191959.1"
                     /translation="LGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTH
                     LRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLLLCALVLSIFGIET
                     RKVSLEEISEVN"
BASE COUNT         5291 a         5860 c         5695 g         5401 t
ORIGIN      
        1 tcagcgccga gacatctgat cgctcaacgg gacggatgac catcatgatg aacctcggct
       61 aacaaagtgt tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct
      121 gacattgagc gcaggcgcaa aacgcaccac gttgccacct gcaatcagta ccatcacgcc
      181 tgctttcgcc gcttcctgag agatctgttt cgcttgcccg gcgtaatcgg cattcagcac
      241 acagccaatc aacaaaccta agccgcgaat ttcgctgaac aaaccgtagc ggtgattaag
      301 ggtattaaga cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg
      361 tgtgttgatg agctccagca ctttgcctgc caccgccgag gccagcggat taccgccata
      421 ggtggtgcca tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc
      481 accgaccggg aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag gcgtcacgcc
      541 gtagtgcata taggcataca gttccccggt gcgcccgacg ccggtttgta cttcatcaaa
      601 aatcaacagc gcattgtggc ggttacacaa ttcacgcaga ccttgtaaaa acgcgttgct
      661 ggctggcacc acaccgcctt ccccctggat gggttcgaca atcaccgcac aggtagagtc
      721 gtcaatcagc gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg
      781 cagtggcgca aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt
      841 gcgaccatga aacgcatttt tgaacgccac gatgccgctc ttatggctgc cgtagcggtc
      901 gtgagcgaat ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca
      961 aaagaagacg cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg
     1021 ctcgttggtg taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc
     1081 acgcagttcc ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat
     1141 atactctttc ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg
     1201 tgccggagcg taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga
     1261 catagcgacc tctacagtaa ataattcgtt atttatatgt taataataag taatgtttgc
     1321 gctgtaaatg tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga
     1381 ggctaactgg aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt
     1441 gaatacgttt gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta
     1501 aaatataatt ctggaattgt gatcattgac gaaatttact ggaaattact gcgccattct
     1561 gacgcagcgc gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca
     1621 tcgatcaaat cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca
     1681 ctctttatct gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct
     1741 gcgcgccaga cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg
     1801 cctgcaggag acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg
     1861 ctacaacgtg ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga
     1921 gacgccgatt gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat
     1981 tattatggcg gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc
     2041 gcagggtgaa agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa
     2101 tctgcaaaac tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga
     2161 tatgaatatc agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg
     2221 gctgcgtacc ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat
     2281 gagctggggg ttggtcgata ccttccgcca tgcgaatccg caaacagcag atcgtttctc
     2341 atggtttgat taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct
     2401 gctcgccagc caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaatccg
     2461 cagcatggaa aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag
     2521 cagctctcct ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt
     2581 cagtaatgat ttgttgcctg ccgtcctttg ttataccgtc tctgcgtttt tagttgtctg
     2641 accacttctc tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaaat
     2701 ttctttttgc ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat
     2761 tcgatctgct caccgattta ccccacttac agacactcat ccgccagagc ggatttttcg
     2821 gctatagcct ctatattctg ttattcatca ttgccaccct cttgctgtta ccaggaagca
     2881 tactggtgat cgcgggagga atagtttttg gcccgctctt agggacacta ctctcattaa
     2941 ttgccgccac gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt
     3001 tactgctgaa atacgttggc catagcaata ccttccaggc cattgaaaaa ggcattgcgc
     3061 gtaacggtat tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc
     3121 aaaattacgc ttacggatta accacaatcg ccttctggcc ttataccctt atttcggcac
     3181 tcacgaccct acccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag
     3241 gcattacgct gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctcg
     3301 tccagctcgc aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca
     3361 gcccacttac tcacccaaaa aatgaaggat agaacgatgt tgcaacatta ttcagtgtca
     3421 tggaaaaaag gactggctgc actctgttta ctggctgttg ctgggcttag cggctgcgat
     3481 caacaagaga atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg
     3541 cgtgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc
     3601 accgacgaca ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg
     3661 tttatgggtt atgcgacccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg
     3721 ccgggcgaaa acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg
     3781 gatatttcgg tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt
     3841 gacagtaatg gtaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag
     3901 aagaaaacgg gttgcctagt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca
     3961 acatacactt atggtgcggt tgaaaaacgt ggtgaagtta aattcaaagg caatgcctca
     4021 gttctcccgg cggataacac gctggcaacg gttaccttta aaatcgccga ataaagccag
     4081 gataaaggat gatgatgatg caatcgcgaa aaatctggta ctaccgtata accctcatca
     4141 tcctgttgtt cgctatgctg ctggcatggg cgctgcttcc cggcgtccat gagtttatca
     4201 atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt
     4261 cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttgcag gccattgctg
     4321 caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg
     4381 gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca
     4441 gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg
     4501 acggcttttt cactcgctac ggcaaacaca ccattctggt ctgtcggtta ttgccttttg
     4561 tccctttcga tccaatcagc tatgctgccg gtttgacttc aatacgtttt cgctcgtttt
     4621 ttatcgccac cgggcttggt cagttaccgg caactattgt ttattcctgg gcgggcagca
     4681 tgttaacagg cggtactttc tggtttgtca ccggactgtt tattctgttt gccctgaccg
     4741 tggtgatttt tatggcgaag aaaatatggc ttgaacgcca gaagaggaat gcctgatggg
     4801 tttaccgccg cttagcaaaa ttcctttaat tttacgtcca caggcgtggc tgcatcgtcg
     4861 ccattacggc gaggtgctaa gccccattcg ctggtggggg cggatcccgt ttatctttta
     4921 tctggtgtcg atgtttgttg gctggctgga gcgcaaacgc tcaccgctcg atccggtagt
     4981 acgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gtgtggatat
     5041 caccagtatg aaagtcgccg agcgcaccgg cagcagcgat aaactgctgg cagtggctga
     5101 ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc
     5161 cgcaagcgta acgccgccaa cggtcgatga tgccctgcgt acccgactgg ctgcgcattt
     5221 tgacgctcag gcgctcaccg aactgacggc attgatcggc ctgcaaaatc tgtcagcccg
     5281 ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattcctg aaaaacgttc
     5341 ttaaggagag atgatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttctct
     5401 ggcaacacat gccagtgact ggcaagaaat taaaaatgag gccaaagggc aaaccgtctg
     5461 gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg tgagcggcga
     5521 gatgaaaacc cattacgcta taaacctgaa gattgtccgt ctggcggatg ccgcagacgc
     5581 ggtgaagcgc attcagaccg aagccgcagc cggacgtaaa acgggcggct cggtggatct
     5641 gctctgggtg aacggcgaaa acttccgcac cttaaaagag gccaatttat tacaaacggg
     5701 ctgggcggag actctgccca actggcgcta tgtcgacaca cagctgccgg tgcgggaaga
     5761 tttttctgtg ccgacacaag gtgcggaatc gccctggggc ggcgcacaac tgacgtttat
     5821 cgcccgccgc gatgttacgc cacagccacc acaaacgccg caagccttac tggagtttgc
     5881 taaagccaat cccggcacgg ttacctatcc gcgcccaccg gactttaccg gcacggcgtt
     5941 tcttgaacag ttgctgatta tgctgacgcc cgatcccgcc gcattaaaag aagcgccgga
     6001 cgatgcgact ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg tgctgcatcc
     6061 gtatttgtgg cgcgaaggaa aggatttccc gccttcaccc gcgcggatgg atgctctgct
     6121 gaaagccggc acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat
     6181 cgccagcggc gatttgcctg caagcagtta cagttttggc tttcgcgagg ggatgattgg
     6241 caacgtgcat ttcgtcacca ttcctgccaa cgcgaatgcc agtgctgcgg cgaaggtagt
     6301 tgccaatttc ctgctctcac ccgatgcgca actgcgtaaa gcagatcccg ctgtctgggg
     6361 cgatccttct gttctcgatc cgcaaaaact gcctgacggg cagcgcgaat cattgcaatc
     6421 aagaatgccg caggatctgc cgccggtact ggctgaaccg cacgcaggtt gggtaaatgc
     6481 gctggaacaa gaatggctac accgttacgg tacgcattaa tctttttgct gtgggcgatg
     6541 gtggcggtga tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgctttg
     6601 tcgttgacac actggcaggc gttatttgcc gatccacagt taccgcaggc attactggca
     6661 acgctggtgt cgacaaccat cgcggcggtc ggggcattgt tgattgccct gctggtgatt
     6721 gtggcgctgt ggcctgggcc gaaatggcag cgtatgtgcg cccgtctgcc gtggctgctc
     6781 gccattcccc atgtggcttt tgccaccagc gcccttctgc tctttgctga cggagggctg
     6841 ctttatgact atttcccgta tttcactccg ccaatggacc gatttggcat cggtctgggc
     6901 ctcacccttg cagtgaaaga aagcgcattt ctgctgtgga tcttagcagc agtattgagc
     6961 gaaaaatggc tgttgcagca ggtcattgtg ctggattcgc tgggctacag ccgctggcaa
     7021 tgcctgaact ggctgctgtt gccctccgtc gcgcctgcgc tggcaatggc gatgctggcg
     7081 attgttgcct ggtcgctgtc ggtcgtggat gtggcaatta ttctcgggcc aggtaatccc
     7141 ccgacgctgg cggtaattag ctggcagtgg ttaacccagg gcgacattga ccaacaaaca
     7201 aaaggcgcgc ttgccagcct gctgttgatg ctgttactcg ccgcctacgt tttgctgagc
     7261 tatctgctat ggcgcagctg gcggcgcact attccccgcg tagatggcgt tcgcaagcct
     7321 gccacgcctt tattgccggg caatacgctg gcgatttttt tacccttaac cggtgtgctg
     7381 tgtgtggttc tgctggcgat cctcgcggat cagtcgacga tcaatagtga agcgctcatc
     7441 aacagcctga caatggggct ggtggcgaca ttcatcgctt tgctcctgtt actgctgtgg
     7501 ctggaatggg ggccacagcg tcgccagttg tggctatggt tgcccatttt attacccgct
     7561 ctgccactgg tggcgggcca gtacacgctg gcgctatggc tgaacctgga tggaagctgg
     7621 acggcggtgg tctgggggca tctgctgtgg gtaatgccat ggatgctgtt tatcctgcaa
     7681 cccacctggc agcgcattga ttcacggtta attttgattg cgcaaacact gggctggtcg
     7741 cgggccaaaa tcttctttta cgtgaaatgt ccgcttatgc tacgccctac gctgattgcc
     7801 ttcgcggtgg gatttgcagt cggtattgcg cagtatatgc caacgctgtg gctgggcgcg
     7861 gggcgttttc cgacgctcac cactgaggcg gtggcattaa gcagcggcgg cagcaacggt
     7921 attctcgccg cccaggcttt atggcaactg ctattaccgc ttattatttt tgccctgacc
     7981 gcgttagtcg caaaatgggt aggttatgtc agacaaggac tccgctaatg ctctgcgtga
     8041 aaaatgtttc gctacgttta ccagaaagcc gcttgctgac aaacgttaac tttacggtgg
     8101 ataaaggtga cattgtcacg ttaatggggc cgtctggctg tggaaaatcc actctgtttt
     8161 catggatgat tggtgcactg gccgaacagt tttcttgtac aggtgagcta tggctcaatg
     8221 agcaacggat tgacatccta cccaccgcac agcgtcagat tggcattctt tttcaggatg
     8281 cactgttatt tgaccagttc agtgtcgggc aaaatttact gctggcgcta ccggcgacac
     8341 ttaaagggaa tgcccgacgt aatgccgtga atgatgcact tgagcgttca ggccttgagg
     8401 gagctttcca tcaggatcct gccactttgt ctggcggtca gcgagcgcgc gttgctctac
     8461 tacgcgccct tctcgcccaa ccaaaagcgt tactcctgga tgagccattc agccgtcttg
     8521 atgtggctct gcgcgataat tttcgccagt gggtgttcag cgaagttcgc gccctggcga
     8581 tccccgtcgt tcaggtaacg cacgatctcc aggatgttcc tgctgatagt tctgttctgg
     8641 atatggcgca gtggtcagaa aattacaaca aactgcgata acgcaaagtt tttctcaatg
     8701 cgtcagttca gaatggcgca ctcaaaacta caatgtcggg attttcgatg aaacgtgttt
     8761 ctcaaatgac cgcgctggca atggctttag ggctggcttg cgcttcttcg tgggccgctg
     8821 aactggcgaa gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa gcgatagata
     8881 ctcgccccag cgcgttttat aacggctggc cacaaacctt aaatggccct tctggtcatg
     8941 aacctgccgc cttaaacctc tctgccagct ggcttgacaa aatgagcacc gaacagctca
     9001 acgcgtggat caagcaacat aacctgaaaa ccgatgctcc ggtggcgctg tacggtaatg
     9061 acaaagatgt cgacgccgtc aaaacgcgac tgcaaaaagc aggtttaacg catatctcca
     9121 tcctgagtga cgcgctaagc gaaccttccc gtctgcaaaa actgccgcat tttgagcagc
     9181 tggtttatcc gcaatggctg cacgacctgc aacaaggtaa agaggttacg gcgaaacctg
     9241 ccggtgactg gaaagtcatt gaagcggcct ggggcgctcc taagctttac cttatcagcc
     9301 atattcccgg cgctgactac atcgatacca acgaagtgga aagtgaaccg ctgtggaaca
     9361 aagtttctga tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc catgacacca
     9421 cggtcattct gtatgggcgt gacgtatacg ctgcagcgcg tgtggcgcag attatgcttt
     9481 atgctggcgt gaaagatgtg cgcctgctgg atggcggctg gcaaacctgg tccgacgcgg
     9541 gactgcctgt tgagcgcgga acgccaccga aagtgaaagc ggaaccggat ttcggcgtga
     9601 agatcccggc acaaccgcaa ctgatgcttg atatggaaca ggcgcgtgga ctgctgcatc
     9661 gccaggatgc atcgctggtg agcattcgtt cgtggccaga atttatcggt acgaccagcg
     9721 gttacagcta tattaaacca aaaggtgaaa tagccggagc acgttgggga cacgctggta
     9781 gcgactcgac gcatatggaa gatttccata acccggatgg caccatgcgt agcgccgatg
     9841 atattaccgc tatgtggaaa gcatggaata tcaaaccaga tcagcaagtt tcattctact
     9901 gcggcaccgg ctggcgcgcg tccgaaacct ttatgtacgc acgagcaatg ggctggaata
     9961 acgtctccgt ttacgacggc ggctggtacg aatggagcag cgatccaaaa aattcggtag
    10021 caaccggtga acgcggcccg gacagcagca aataacattg caattactga cgctggagcg
    10081 acttcagcgt cagataccca ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac
    10141 caaataccca cgcaaaccac ggaaaacagg tcggaaagag gcaactcaga acaaacagca
    10201 aaatcgtctc gctgccttcg gttaacccgc ccagataata aaacgattta tgcgcataac
    10261 cgggattatc aatctgatgt ttcgccgcca gcgcagcaaa agcgagaaaa ctgctacccg
    10321 tgccgataaa cgcaaacagc agccagccgc ctgccagggc gttttgctgc ggtgcggcga
    10381 ggataaaccc aaaaggcacc agcgcgtaaa aaagaaaatc gagagaaata tcaagaaagc
    10441 cgcccgcgtc ggtaagccct ctgcgccgcg ccagcgcacc gtccagacca tcaagcagcc
    10501 tgttcaacaa aatgacgact agcgccgcca gataccagcc cagcgccaga aacggcagag
    10561 ccagcacgcc gatggcaaat cccactaatg ttaaaccatc tggcgtaatg cccggtttat
    10621 caagaacccg cacgcactga tgcaacaacg gtttaatccg gggatgaaga tggcggtcta
    10681 gcacaggcac tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc
    10741 atttttcgct tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc
    10801 attgaacgtg atggcaaaat tttactcgcg caacgccccg cccagcgcga tcaggcggga
    10861 ttatgggagt ttgccggtgg taaagtcgag ctggatgaaa gccagcagca ggcgctggtg
    10921 cgtgagttaa acgaagaatt gggcatcgaa gcaactgtgg gtgagtatgt tgccagccat
    10981 cagcgagaag tttcggggcg gattatccat cttcatgcct ggcacgtacc cgacttccac
    11041 gggacgttac aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg
    11101 caatatccgc tggcccctgc tgacattcca ttattagagg cgtttatggc tttacgcgcc
    11161 gccagaccag cggattagtg ctaagggttt tgtcatcacg ctggcattgc agcagtattc
    11221 cttcggcttt aattaccgcc ccttcagaat aattttgatc ctgataaacg cagcactgag
    11281 tacagggctg cgctgactgc ccgcctgaac tgaacacttc tggcggtacg tttacctcca
    11341 cgtccggacg ataatgcggg ttagccagtg caattaatgg aaatgctaat actacggcga
    11401 acaatgctcg actcacaggg aactccttaa cgttattgtc tctgctactg ataacggtag
    11461 ccgggtggca aaactttagc gtctgagtta tcgcatttgg ttatgagatt actctcgtta
    11521 ttaatttgct ttcctgggtc atttttttct tgcttaccgt cacattcttg atggtatagt
    11581 cgaaaactgc aaaagcacat gacataaaca acataagcac aatcgtatta atatataagg
    11641 gttttatatc tatggatcag acatattctc tggagtcatt cctcaaccat gtccaaaagc
    11701 gcgacccgaa tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc acactctggc
    11761 cttttcttga acaaaatcca aaatatcgcc agatgtcatt actggagcgt ctggttgaac
    11821 cggagcgcgt gatccagttt cgcgtggtat gggttgatga tcgcaaccag atacaggtca
    11881 accgtgcatg gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc ggtatgcgct
    11941 tccatccgtc agttaacctt tccattctca aattcctcgg ctttgaacaa accttcaaaa
    12001 atgccctgac taccctgccg atgggcggtg gtaaaggcgg cagcgatttc gatccgaaag
    12061 gaaaaagcga aggtgaagtg atgcgttttt gccaggcgct gatgaccgag ctttatcgtc
    12121 atctgggcgc ggataccgac gttccggcag gtgatatcgg ggttggtggt cgtgaagtcg
    12181 gctttatggc ggggatgatg aaaaagctct ccaacaatac cgcctgcgtc ttcaccggta
    12241 agggcctttc atttggcggc agtcttattc gcccggaagc taccggctac ggtctggttt
    12301 atttcacaga agcaatgtta aaacgccacg gtatgggttt tgaagggatg cgcgtttccg
    12361 tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa tttggtgctc
    12421 gtgtgatcac tgcgtcagac tccagcggca ctgtagttga tgaaagcgga ttcacgaaag
    12481 agaaactggc acgtcttatc gaaatcaaag ccagccgcga tggtcgagtg gcagattacg
    12541 ccaaagaatt tggtctggtc tatctcgaag gccaacagcc gtggtctcta ccggttgata
    12601 tcgccctgcc ttgcgccacc cagaatgaac tggatgttga cgccgcgcat cagcttatcg
    12661 ctaatggcgt taaagccgtc gccgaagggg caaatatgcc gaccaccatc gaagcgactg
    12721 aactgttcca gcaggcaggc gtactatttg caccgggtaa agcggctaat gctggtggcg
    12781 tcgctacatc gggcctggaa atggcacaaa acgctgcgcg cctgggctgg aaagccgaga
    12841 aagttgacgc acgtttgcat cacatcatgc tggatatcca ccatgcctgt gttgagcatg
    12901 gtggtgaagg tgagcaaacc aactacgtgc agggcgcgaa cattgccggt tttgtgaagg
    12961 ttgccgatgc gatgctggcg cagggtgtga tttaagttgt aaatgcctga tggcgctacg
    13021 cttatcaggc ctacaaatgg gcacaattca ttgcagttac gctctaatgt aggccgggca
    13081 agcgcagcgc ccccggcaaa atttcaggcg tttatgagta tttaacggat gatgctcccc
    13141 acggaacatt tcttatgggc caacggcatt tcttactgta gtgctcccaa aactgcttgt
    13201 cgtaacgata acacgcttca agttcagcat ccgttaactt tctgcgatag cagcagatat
    13261 gccagtaaag aaatcccatt tgactatttt tttgataatc ttcttcgctt tcgaacaact
    13321 cgtgcgcctt tcgagaagca agcattatat aatgccaggc cagttcttct tcaattgtcc
    13381 cgttttgaaa agctgtgctt gatatcgaga tcatccatga taattccgcc gcccatatta
    13441 gcttcgccga ggatttaccg gagctatgat tagcgcaatc agagatatag tctgagggaa
    13501 aaacagcaaa tttattcaac aaggcgataa cctgctctgg ggcttcctcc atgtttgctt
    13561 taaaggtatt ggctccatgg tcgccagaaa gaaaatgctc cattaaggca caataacttt
    13621 cgctatcttc gataccccat tgatcctcta aagactcgcg tcttttactt atgatatcga
    13681 tcgagtcaaa aggaagcaca tgatattgga aggtatcttt gccaggttca ggctttcgcg
    13741 gccagaactc cagcgtttca gaccattgct tatgatagaa tcgataaggt gcgatcaatt
    13801 gtagcgcctg taacttctcg atactgagcg gctcaatacc tttagcctga taataatgca
    13861 gttgttcttt ttttgcttta aaaccggccc gaacaataag ccccatcata attaatagat
    13921 aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt caaaccgttg gatgttatcg
    13981 ctgcgaacac aaacattaca gcgacaacac atgttaaata aaacccccac ttacaaagca
    14041 gcatggcctt attttcttta atcatccgtt caaaattact attaaatatt tcccagccat
    14101 taaaagaata cttctcgctc ccaggatggt tttgtaataa aacttttttc atcacattcc
    14161 ctgttattac atactttaga ggcgataatt ttatccatgc aaaaaaaata tccaacgaat
    14221 tttcttgatc cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcactcgct
    14281 tacgctatcg ccccgcttcc gacttcatct gctggcggac tttttttcgc actacgttta
    14341 cgcggtgcag cctttttctt atcagcactg ccaccactgc ccggagccac aatgccgcga
    14401 aactgccgca ccggcgtacg tttggcttga tcaataagct gatatagcgt ccccaccagc
    14461 ggctgcataa agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc
    14521 cagtgcgcgg tcatgtccgg tcgcgtcgcc atctccggca gcgaatggaa tagcgctttt
    14581 ccggcgtcgg tggagtggat atagcgccct tttttggtca ggaaaccacg cttgaacaac
    14641 agttcaataa tcccggcacg cgttgcctct gtccccagac catcggtcgc acggaggatc
    14701 tttttcagat ctttatcctg cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt
    14761 gcatcggtaa aatggcgcgg cggctgggtt tgccgctcta ccacttcacc tttttcacac
    14821 agcaactcat cgcctttcgc caccacaggc agtggcgtgc cgtcgttttc ttcatcgcgc
    14881 tctttgctgc ctaacagcgt gcgccagcct gcttcagcaa gaaaacgcgc tttagcgaca
    14941 aatttgcctt tggcaatgtc cagttcgata acacacttgc ggaacaccgc atccgggcag
    15001 aattgcatca gatactgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg
    15061 ttgatcgcag aactccgtgc ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca
    15121 tcccaacagc ggttgcgtat atctggatct accactggct gcggcaacag atccggtgca
    15181 tgaacactga tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag
    15241 cgacaatcag aacgcggata agtgattagc ttgtgcgttt cgtacagttt ctggcagata
    15301 tcaagcacgt tctgcgcgct cagaccaaag cgttttgccg cttcaatctg caacgccgaa
    15361 agcgaaaacg gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaata
    15421 gccggttgac cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc
    15481 ccttcttcat cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc
    15541 tcatcggcag gtgtcacgat atgcgctttg acttcaaaga agtctttcgc cacgaagttt
    15601 tcaatctctt catcgcggcg caccaccagc ccgagcacgg gtgtctgcac gcgccccacg
    15661 gaaagcacgc cctgataacc agcattgcga ccgagaatgg tgtaagcacg ggtcatattg
    15721 atgccgtaca gccagtcggc acgcgctcgc gccagcgcag aaacgcacag cggtacaaac
    15781 tcactgttgg aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt
    15841 atcaagcaac gctgcacctg ctggcgcttt tccggtgcca gttgcagata gtccagcact
    15901 tcatccacca gcaattgccc ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct
    15961 tcatgcagga agcgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat
    16021 tgccactttt ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg
    16081 taggcgtctg gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca
    16141 ttaccgcact cgataaagcc atcgcctttc cggtgcggtt tgggcaggac atcagcaatg
    16201 gcgcgcgcca gactcggttt ttcggcaata aacaaccgca ttgaattaac gaatctcaac
    16261 catggcacga ccgccgcgcg ctggcaccag ttcgccaatt gccgtcagtt caatgccaaa
    16321 ctcggcggct gtagctttga cctcattttc tgcttccggc atgaccgcca gcagcaaacc
    16381 gccagaagtt tgcggatcgc acagcagatc gcgcacttca cgcggcattt cacccatcag
    16441 atgaccgtag ctggcaaagt tacgttcagt gccgccaggt actgcgccca acttaatgta
    16501 ctcttcaaca ccggggagtt tcgggatcgc ttcatagtcg acgcgtgcct gcacaccagc
    16561 cccctgacac atttcgctca agtggcccag cagaccaaag cccgtaacgt cggtcatcgc
    16621 ttttacgcct tcgatgttgg caaaggacgc gcctgcgatg ttcatccggc acatcacttc
    16681 cgtcgccagt ccctgatgtt ctggtttcaa cagtgatttt ttctcagccg tggtaagaac
    16741 gccgatcccc agcggtttcg tcaggaacag tttgcatccg gcttgtgcag tactgttttt
    16801 cttcacccgc tcggtcggta cgatccccgt taccgccaga ccaaaaatcg gctccggcgc
    16861 atcgatggag tgaccgccag ccagcgcaat acccgcctga cgacatgcat agcgtccacc
    16921 ttcggtcact tcgcgggcaa tttccgggga aagtttgtta atcggccagc cgaggatcgc
    16981 aatcgccata atcggtttgc cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc
    17041 aatgcggcca aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat
    17101 aacgctggtg ccattgccca gatcgtacac cgccgcatcg tcgcgggttt cattacccac
    17161 aagcaaattc ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt
    17221 tggggaaatt ttacagccgc aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt
    17281 ctcgctcatg gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag
    17341 tgataagtga gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac
    17401 aattatcaga aataagttac aaacggcgtc gggtccggga cgttaatcga cgtagatgct
    17461 ttcagctgcg gcgtaccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg
    17521 aatgcttcac gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaac
    17581 ccctgggcaa ctgctgccat ttgcatcgcc atgaccgcgc atccggcaga catttcctgt
    17641 tcccagcgcg ggactttatg attctcttcg catttcgcca ccaccgtgat gatgagcggt
    17701 gcgcggaacg gcgcattacg ggctttgtcg atagctttgt catcactacc ggcagcaatc
    17761 gccccctgtt ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa
    17821 tgccacggtt gcatggactt atggtccggc gcacgcatac ccgcacgcag gatgttttgc
    17881 agttgttcac ccgttggcgc gggttcagcc aagcgggagg cgctacggcg attgatcaat
    17941 agttcgagtg catccatttg attaactcct gtcgtgatat ttattcacaa aattaacacg
    18001 agagtggatt ttgttacagc acagtccgca attcctgctg acaagtaccg gttgggtcat
    18061 tacgataacc acatctattg cgcctgtgac aggtgtgacc ttaagttggg agaatacatg
    18121 cgaacccttt ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc
    18181 gtccgtgaaa tggtacttaa cctgttcttt attttcctcg tactggttgg tgtggggatt
    18241 tggatgcagg tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg
    18301 gacatttctg gtgtgatcgt cgataaaccc gacagttctc agcggtttag taaattaagc
    18361 cgccagctgc ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc
    18421 aacactattc gccaggcgaa ggacgaccgc aatatcaccg gtattgtgat ggatctgaaa
    18481 aacttcgcag gcggcgacca accgtctatg cagtacatcg gcaaagctct gaaagagttt
    18541 cgtgacagcg ggaaaccggt ttatgccgtt ggcgagaact acagccaggg gcaatattat
    18601 ctcgccagtt tcgccaataa aatttggctg tctccgcaag gcgtggttga tctgcacggc
    18661 tttgccacca acggtctgta ctacaaatcg ttgctggata agctgaaagt ttccacccat
    18721 gtgttccgcg tgggtacgta taaatctgcc gttgaaccgt ttattcgtga tgatatgtca
    18781 ccggcagccc gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat
    18841 actgttgccg ctaaccggca gatccctgct gagcaggtat tccctggcgc gcaagggttg
    18901 cttgagggtt taaccaaaac cggtggcgat accgcgaaat atgcactgga aaacaagctg
    18961 gtcgatgcac tggcatcgag tgcggaaatc gaaaaagcac tgaccaaaga attcggctgg
    19021 agtaagactg ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg
    19081 gcagataccg gtgacagcat cggtgtcgtc tttgctaatg gcgcaattat ggatggcgag
    19141 gaaactcagg ggaatgttgg cggtgatacc actgcggcac aaatccgcga cgctcgcctt
    19201 gacccgaaag tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg
    19261 tctgaagtga ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta
    19321 tcgatgggcg gcatggcggc atctggtggt tactggattt ccacgccagc taattacatt
    19381 gtggctaacc ccagcaccct gaccggttct atcggtatct tcggcgtgat caccaccgta
    19441 gaaaatagtc tggattcgat tggtgttcat accgatggtg tctcaacttc accgctggca
    19501 gatgtttcta tcaccagggc actgccgccg gaagcacagc agatgatgca gttaagcatt
    19561 gagaatggct ataaacgctt tatcacgctg gttgctgatg cgcgtcattc gacgccggag
    19621 cagattgata aaatcgccca gggccacgtc tggaccggtc aggatgcaaa agctaacggg
    19681 ctggtcgata gcctcgggga tttcgatgat gcagttgcca aagcagcaga gctggcaaaa
    19741 gtgaaacagt ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg
    19801 gacaacatgt ctggttctgt ccgggcaatg ttgccagatg cgttccaggc catgttacct
    19861 gcaccgctgg cctcggtagc ctctactgtt aaaagtgaaa gcgacaagct ggccgcgttt
    19921 aacgacccac aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg
    19981 tactgagtgg ccgacagatc gtcggccaca ttatttttta cgtcgacgaa tcctcttccc
    20041 gctgtctcgc cccatatttc cttatccgca tagtatcagg tgcgctcctc ctgcctcacg
    20101 tatatacttt tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta
    20161 cgtcgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt atattccggt
    20221 gtcaggtcat ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc
    20281 agatttcacc attcatgaat atacgccgtt gatggattct tctgatatga ctccagaaga
    20341 ctggcagcat attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat
    20401 tctgcacggc accgatacga tggcatatac cgcctctgcg ctgtcgttca tgctcgagaa
    20461 tctcggtaaa ccagtcattg tgacagggtc acaaatcccg ctagctgagt tacgctctga
    20521 cggacaaatt aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt
    20581 aacgctcttt ttcaataacc gattgtatcg cggcaaccgc actaccaaag cccatgccga
    20641 tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagcag gtatccatat
    20701 tcgtcgttta aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat
    20761 caccccacaa ccaattggcg tagtgacgat ttatccaggg atttctgctg acgtcgtgcg
    20821 caattttctg cgccaaccgg tgaaagcatt gattttgcgc tcctatggcg tgggtaatgc
    20881 gccacaaaac aaagccttcc tgcaggaatt acatgaagcc agcgatcgcg gtattgtggt
    20941 ggtcaacctg acacaatgta tgtccggtaa agtgaacatg ggtggttatg ccaccggtaa
    21001 cgccctcgcc catgccggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac
    21061 caaactgcat tacctgctga gccaggaact ggatactgaa accattcgca aggccatgag
    21121 ccaaaacctg cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccctcgcg
    21181 ccctgttact ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg
    21241 aaggtgacag tacggtggat gtcgctaacc gcctgattga ctggtgccag tcgcgcggtg
    21301 aagcggttat cgccagtcag gactggcacc cggcgaatca cggcagtttt gccagtcagc
    21361 acggtgtaga gccttatacg ccaggccaac tcgacggttt gccacaaacc ttctggccag
    21421 atcactgtgt gcagaacagt gaaggcgcac aattacatcc gttactgcac caaaaagcga
    21481 tcgcagcggt gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg
    21541 ataacggccg tcggcagaaa acctctctcg atgactggtt acgcgatcat gaaatcgatg
    21601 aattgatcgt tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggacgcgt
    21661 tacagttagg ttataaggta aacgtgatta ccgatggttg tcgtggcgtg aatatccagc
    21721 cacaggacag tgcgcacgcg tttatggaga tgtcagctgc tggggcaacg ctatatacgc
    21781 tggcagactg ggaagagaca caggggtaat tttacgctgg cctacaattc tgtactggca
    21841 ttgtaggcca aataaaacac gtcagtggca catctggcaa ttgatgccat caacgaaaga
    21901 tttaattcac ctcagaaatc tcttccaacg acaccttccg cgtttcgatg ccaaaaatgg
    21961 agagaaccag cgcacagagc aataacatga caccaagtac tataaacacc gtgatcgacc
    22021 cataatgtgt taataatgcc gcaacgccat agggcgtgaa gactgcgacg atccgtccga
    22081 cggcattaac gaaacccgaa ccgcgcaggc gtaaatgcgt tggccaaagc tccgggatat
    22141 aaaccgccga cgcgaagcaa acatacatgt ataaaaagaa gatcatcacc aggccataga
    22201 ttaaaatcgc ccactctgta gtctgaattg aatagatata gcctaac
//
LOCUS       DACAPB010000064        22243 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.064, whole genome shotgun sequence.
ACCESSION   DACAPB010000064 DACAPB010000000
VERSION     DACAPB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.064"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(77..151)
                     /locus_tag="IT904_002184"
     CDS             complement(77..151)
                     /locus_tag="IT904_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191960.1"
                     /translation="MRDILLNVLNIVFIGIAIILVIIC"
     gene            complement(457..2490)
                     /gene="betT"
                     /locus_tag="IT904_002185"
     CDS             complement(457..2490)
                     /gene="betT"
                     /locus_tag="IT904_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HAP3191961.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2619..3206
                     /gene="betI"
                     /locus_tag="IT904_002186"
     CDS             2619..3206
                     /gene="betI"
                     /locus_tag="IT904_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HAP3191962.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKTRANSLTRHFITQHLPTD"
     gene            3220..4692
                     /gene="betB"
                     /locus_tag="IT904_002187"
     CDS             3220..4692
                     /gene="betB"
                     /locus_tag="IT904_002187"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HAP3191963.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPNGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4706..6376
                     /gene="betA"
                     /locus_tag="IT904_002188"
     CDS             4706..6376
                     /gene="betA"
                     /locus_tag="IT904_002188"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HAP3191964.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     IGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6589..7257
                     /locus_tag="IT904_002189"
     CDS             6589..7257
                     /locus_tag="IT904_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191965.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7500..8195)
                     /locus_tag="IT904_002190"
     CDS             complement(7500..8195)
                     /locus_tag="IT904_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAP3191966.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(8188..9615)
                     /locus_tag="IT904_002191"
     CDS             complement(8188..9615)
                     /locus_tag="IT904_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAP3191967.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9626..10345)
                     /locus_tag="IT904_002192"
     CDS             complement(9626..10345)
                     /locus_tag="IT904_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAP3191968.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10872..11726)
                     /locus_tag="IT904_002193"
     CDS             complement(10872..11726)
                     /locus_tag="IT904_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP3191969.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11952..13277
                     /locus_tag="IT904_002194"
     CDS             11952..13277
                     /locus_tag="IT904_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAP3191970.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRMLGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13386..13622
                     /locus_tag="IT904_002195"
     CDS             13386..13622
                     /locus_tag="IT904_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP3191971.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13634..14227
                     /gene="rclC"
                     /locus_tag="IT904_002196"
     CDS             13634..14227
                     /gene="rclC"
                     /locus_tag="IT904_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAP3191972.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14387..14515)
                     /locus_tag="IT904_002197"
                     /pseudo
     CDS             complement(14387..14515)
                     /locus_tag="IT904_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            14818..15669
                     /locus_tag="IT904_002198"
     CDS             14818..15669
                     /locus_tag="IT904_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3191973.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(15809..20065)
                     /gene="fdeC"
                     /locus_tag="IT904_002199"
     CDS             complement(15809..20065)
                     /gene="fdeC"
                     /locus_tag="IT904_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HAP3191974.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTMKF
                     KADFSTGQATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATMTVTLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSK
                     PGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP"
     gene            20619..20708
                     /locus_tag="IT904_002200"
                     /pseudo
     CDS             20619..20708
                     /locus_tag="IT904_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            21182..21283
                     /locus_tag="IT904_002201"
     CDS             21182..21283
                     /locus_tag="IT904_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191975.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFH"
     gene            21647..21910
                     /locus_tag="IT904_002202"
     CDS             21647..21910
                     /locus_tag="IT904_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP3191976.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            21910..22050
                     /gene="rpmJ"
                     /locus_tag="IT904_002203"
     CDS             21910..22050
                     /gene="rpmJ"
                     /locus_tag="IT904_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP3191977.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(22085..>22243)
                     /locus_tag="IT904_002204"
     CDS             complement(22085..>22243)
                     /locus_tag="IT904_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3191978.1"
                     /translation="YRGEAGQQVNIKIMEYSERNVRQLASNEQEEYIPRKINVGVINT
                     PTLIRSDY"
BASE COUNT         5812 a         5625 c         5156 g         5650 t
ORIGIN      
        1 aaagatgttg gttatgcata cgtatcatta ctctatgcct gtcccttaaa aacccactca
       61 ttaagactga atccattcag caaatgataa ccaatattat agcaatgcca ataaaaacaa
      121 tatttaaaac attcaataaa atatcacgca cagataatta aaccttaaat aacaaatggt
      181 tgcgttaatt tcagttgatt tacgatacac atttctcatg aataatgcgt catttcaaat
      241 gatcagcaat atttacatta aataagacta aacttattga acattcgcaa cagcccaaat
      301 gtaggttttg ggctgcaata gttcttttct tttttattat ctgttgtctg gtaatcatca
      361 atagatacta agggcttaac cgtatagata taacgtatca agaggtagga gaaacagcgc
      421 catatgcaga catggcgcgg ttttatgcaa taacaatcac gcgtccggga acatcacgct
      481 atggcccggc gcttcacgat ggagatgaat aaagttaagg tgccgctcgt actggtcaag
      541 aatatcggtg atcacctgct ctttgctgta gtccatcagg tcgttgccct ggctgccttc
      601 taacaggaag gtttccagcc ggtagtaggt cgatttaccg ctgcgtgcgc ggtaggtaaa
      661 gcccggcacc gaatattgct gcggccaaat ctgatagaca aagttttgct cttcgcccat
      721 atgcaccaac aaatccagat gacccaactg ctgtccctct tccggtggca ggctttttag
      781 ctccacgtac gcgccgcgca accgcaactc ctgcgccact tcttccattg ccgggtaaca
      841 gaccgtctcc atcatctgtt tagtgtaacg cgtgcccgga taattcatca ggcgcgagag
      901 acgttttttc cagctcaggc gatcctgaag ccccagcggt cgcggtgcgg tatcgcggtt
      961 ggcactttca cggcggtaat cttctacctt cagagattta tacaaccccg ccatcacgaa
     1021 gaagatcaca aagctgaacg gcagccccat aatcaccgtg gtgttttgca gcgcggatat
     1081 cccgttagtc atcagcatgc cgagcgtcag caggccaatc gccaccgacc agaagacgcg
     1141 cagccagccg ggggcgtcgc tgttgatatc tttaagctgc gaggtgaaat tccccagcac
     1201 cagcgccccg gagtccgccg aggtcacata aaacagcagg ccagtaatgg tggcgacgga
     1261 ggcgctaaag gtaaacgccg gatactgcgc cagcaggctg tagaagccgc gctccggatg
     1321 gaccatcgct tcctcggcaa atgccgcgcc gccgtggatg atttcataca gcgcgctatt
     1381 gccgaacacc gagagccata acagcgtgaa ggtaaacgga ataatcaacg tgcccagcac
     1441 gaactggcga atggtacgcc cacgcgagat acgcgccagg aacaagccga caaacggcga
     1501 ccatgccacc caccatgccc agaagaagag cgtccagtta ttcatccact caaccggacg
     1561 gtcgaaggca aaactgttga gcgtcatgcc cataaagcga ttcacatagt cgccaacatt
     1621 cagcaccagt gcattaagca ggaacgaagt gtcgcccata aacaatacga acaggatcaa
     1681 tcccagcgcc agcgcgacat taagctccga taacacgcga atgcccttat cgacaccgga
     1741 ggtgacagag atcgtggcga ttatcaccga caaggcgatc agtgccgctt tcgccgccat
     1801 cgaatcggga atatcaaaca gtacgctcaa gccatagtta agctgcacca caccgatacc
     1861 gagcgtagtg gcaataccga agatagtgcc gatcaccgct gcaatatcca ctgagtgacc
     1921 tatcggcccg ttaatccgtt taccgaagat cgggtacagc gccgagcgga tggtgagcgg
     1981 caaattataa cgatagctaa agtatccgag cgccatgccc atcagcgcat acatcgacca
     2041 gccggttaag ccgtagtgaa acagcgtcca gaccatcgcc tgacgcgcgg cctcaatcgt
     2101 ctgtcccgcg ccttccggcg gctgcatata ctgcgttacc ggttcggcta cggagaagaa
     2161 catcaggtcg ataccgatcc cggcagcaaa cagcatcgcc gcccaactca gcaggctgaa
     2221 ttccggtttg gattgttctg gcccgagctt caccgaacca aaacgcgaac aagcgataca
     2281 gaccacaaag acaatataga gcgttgccgc cagcagatag taccaaccga aggttttaga
     2341 aacccagtcc agcgtgcggc caatccacag ggccgagaag tcgcgaaaca ggatcgttgt
     2401 cagggaaaac aacaaaatca gtccggcgga ggtgtaaaac accaccggat tgattttgtc
     2461 cttttccctg ctgtgtgaaa ggtctgtcat ccagtatccc cactgttatt gttactattt
     2521 aaaatcaaat tcgtaacaat taagacacat tttatattga acgtccaatc aataaccgct
     2581 ttaatagata aacaccgctg atgaatggag tggcgaaaat gcccaaattg ggaatgcagt
     2641 cgatccggcg cagacaactg atcgacgcca cactggaagc aataaatgaa gtgggcatgc
     2701 acgatgcaac gatcgcgcag atcgcccgcc gtgcaggcgt ttctacgggg atcatcagcc
     2761 actatttcag ggacaaaaat ggtctgctgg aagcaaccat gcgcgatatc accagtcagc
     2821 tgcgtgacgc ggttttgaat cgattacatg cacttccgca gggcagtgca gagcagcgat
     2881 tacaggcgat tgttggcgga aacttcgatg aaacgcaggt gagcagtgcg gcgatgaaag
     2941 cctggctggc gttctgggcc agcagtatgc atcagccgat gctctatcgt ttacagcagg
     3001 tcagcagccg ccgcttgctg tcgaatctgg tgagcgagtt tcgtcgcgaa ttgccgcgcg
     3061 aacaggcaca ggaagcgggc tacggcctgg ccgcgctgat tgatggatta tggctgcgcg
     3121 cggctctgag cggcaaaccg ctggataaaa cccgcgctaa ttccctgacc cgccacttta
     3181 tcactcagca tctacccacc gattaaccga ggagacgtga tgtcccgaat ggcagaacag
     3241 cagctttata tacatggtgg ttatacctcc gccaccagcg gtcgcacctt cgagaccatt
     3301 aacccggcca acggtaacgt gctggcgacc gtgcaggccg ccgggcgcga ggatgtcgat
     3361 cgcgccgtga aaagcgccca gcaggggcaa aaaatctggg cgtcgatgac cgccatggag
     3421 cgctcgcgta ttctgcgtcg ggccgttgat attctgcgtg aacgcaatga cgaactcgca
     3481 aaactggaaa ccctcgacac cggaaaagca tattcggaaa cctcaaccgt cgatatcgtt
     3541 accggtgcgg acgtgctgga gtactacgcc gggctgatcc cggcgctgga aggcagccag
     3601 atcccgttgc gtgaaacgtc ctttgtgtat acccgccgcg aaccgctggg cgtagtggca
     3661 gggattggcg catggaacta cccgatccag attgccctgt ggaaatccgc cccggcgctg
     3721 gcggcaggca acgcaatgat tttcaaaccg agcgaagtta ccccgcttac cgcgttaaag
     3781 ctggctgaaa tttacagcga agcgggcctg ccgaacggcg tatttaacgt gttgccgggc
     3841 gtgggcgcgg agaccgggca atatctgacc gagcatccgg gcattgccaa agtgtcattt
     3901 accggcggtg tcgccagcgg caaaaaagtg atggctaact cggcggcctc ttccctgaaa
     3961 gaagtgacca tggaactggg cggtaaatca ccgctgatcg ttttcgatga tgcggatctc
     4021 gatctcgccg ccgatatcgc catgatggca aacttcttca gctccggtca ggtgtgtacc
     4081 aatggcaccc gcgtcttcgt tccggcgaaa tgcaaagccg catttgagca gaaaattctg
     4141 gcgcgcgttg agcgcattcg cgcgggcgac gttttcgatc cgcaaactaa cttcggcccg
     4201 ctggtcagct tcccgcatcg cgataacgtg ctgcgctata tcgccaaagg caaagaggaa
     4261 ggcgcgcgcg tactgtgcgg cggcgatgta ctgaaaggcg atggcttcga taacggcgca
     4321 tgggttgcac cgacagtgtt caccgattgc agcgacgata tgaccatcgt gcgtgaagag
     4381 atcttcgggc cagtgatgtc cattctgacc tacgagtcgg aagacgaagt cattcgccgc
     4441 gctaacgata ccgactacgg cctggcggcg ggcatcgtga cagcggacct gaaccgcgcg
     4501 catcgcgtca ttcatcagct ggaagcgggt atttgctgga tcaacacctg gggcgaatcc
     4561 ccggcagaga tgcccgttgg cggctacaaa cactccggca ttggtcgcga gaacggcgtg
     4621 atgacgctcc agagttacac ccaggtgaag tccatccagg ttgagatggc taaattccag
     4681 tccatattct aaccaggagg tttatttgca atttgactac atcattattg gtgccggctc
     4741 agccggcaac gttctcgcta cccgtctgac tgaagatccg aatacctccg tgctgctgct
     4801 tgaagcgggc ggcccggact atcgctttga cttccgcacc cagatgcccg ctgccctggc
     4861 attcccgcta cagggtaaac gctacaactg ggcctatgaa acggaacctg aaccgtttat
     4921 gaataaccgc cgcatggagt gcggacgcgg taaaggtctg ggtggatcgt cgctgatcaa
     4981 cggcatgtgc tacatccgtg gcaatgcgct ggatctcgat aactgggcgc aagaacccgg
     5041 tctggagaac tggagctacc tcgactgcct gccctactac cgcaaggccg agactcgcga
     5101 tatgggtgaa aacgactatc acggcggtga tggcccggtg agcgtcacta cctccaaacc
     5161 cggcgtcaat ccgctgtttg aagcgatgat tgaagcgggc gtgcaggcgg gctacccgcg
     5221 cacggacgat ctcaacggtt atcagcagga aggttttggt ccgatggatc gcaccgtcac
     5281 gccgcagggc cgtcgcgcca gcaccgcgcg tggctatctc gatcaggcca aatcgcgtcc
     5341 taacctgacc attcgtactc acgctatgac cgatcacatc atttttgacg gcaaacgcgc
     5401 ggtgggcgtc gaatggctgg aaggcgacag caccatccca acccgcgcaa cggccaacaa
     5461 agaagtgctg ttatgtgcag gcgcgattgc ctcaccgcag atcctgcaac gctccggcgt
     5521 cggcaacgct gaactgctgg cggagtttga tattccgctg gtgcatgaat tacccggcgt
     5581 cggcgaaaat cttcaggatc atctggagat gtatctgcaa tatgagtgca aagaaccggt
     5641 ttccctctac cctgccctgc agtggtggaa ccagccgaaa atcggtgcgg agtggctgtt
     5701 tggcggcatt ggcgttggtg ccagcaacca ctttgaagca ggtggattta ttcgcagccg
     5761 tgaggaattt gcgtggccga atattcagta ccatttcctg ccagtagcga ttaactataa
     5821 cggctcgaat gcagtgaaag agcacggttt ccagtgccac gtcggctcaa tgcgctcgcc
     5881 aagccgtggg catgtgcgga ttaaatcccg cgacccgcac cagcatccgg cgattctgtt
     5941 taactacatg tcgcacgagc aggactggca ggagttccgc gacgcaattc gcatcacccg
     6001 cgagatcatg catcaacccg cgctggatca gtatcgtggc cgcgaaatca gccccggtgt
     6061 cgaatgccag acggatgaac agctcgatga gttcgtgcgt aaccacgccg aaaccgcctt
     6121 ccatccgtgc ggtacctgca aaatgggtta cgacgagatg tccgtggttg acggcgaagg
     6181 ccgcgtacac gggttagaag gcctgcgtgt ggtggatgcg tcgattatgc cgcagattat
     6241 caccgggaat ttgaacgcca cgacaattat gattggcgag aaaatagcgg atatgattcg
     6301 tggacaggaa gcgctgccga ggagcacggc gggatatttt gtggcaaatg ggatgccggt
     6361 gagagcgaaa aaatgagtcg tgatgtgaac taacgcagga accgatcaga ttgagagtta
     6421 ccgttccaga gagggggacc gaatccttat ataaacactg aggtaactct catgcttcat
     6481 atccagtatt catgatgcgg gctttttgct gatatttaac ccgaacattc ctgatacaga
     6541 ctattaatac aaccttatct gcgacagatt ttattctctg gatatattat gcgtgaacaa
     6601 atcaaacagg atatcgatct gattgagatt ttattttatc tgaagaaaaa gattcgtgtt
     6661 atccttttta ttatggctat atgtatggct atggtgctgt tgtttctgta tatcaataaa
     6721 gacaatataa aagtgattta cagcctaaaa ataaaccaga caacgccagg tatacttgtt
     6781 agctgtgata gcaataataa ttttgcctgt cagactacaa tgactgaaga tgttattcag
     6841 cgaattacta cattttttca caccagccca gatgtcaaga acagagaaat aaggctggaa
     6901 tggtcaggag ataagagagc tttaccaact gctgaagagg aaatatctcg cgtgcaggcc
     6961 tctattatca aatggtatgc gtcagaatat cataatggca ggcaagttct cgatgagata
     7021 caaacgcctt cagcaattaa cagtgagctt tatacaaaaa tgatatacct gaccaggaac
     7081 tggtcattgt atccgaacgg tgatggctgt gtaactataa gctcaccaga aataaaaaat
     7141 aaataccctg ctgccatttg cctggctctg ggattttttc taagcattgt aatttctgta
     7201 atgttttgcc ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa
     7261 acaacattct gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac
     7321 ccgttttcag gaatggatgc ctgtcttttt cactgtaaat atcctggcat tggtaatacc
     7381 aggatattta cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtccta
     7441 aacgctattt tcaacctcgt atgccttctt caggtttatg tccagacttc atatctctct
     7501 caacaatcct caataatcag atacaccgct ttcaccgggc catgaactcc gacgactttg
     7561 ataagctcaa tatccgccgt tgaactgggg ccgctaatga tgttaatgca ggaaggcatt
     7621 cgttcaccgg cctgcgcttt ctgatgcaat ttttctgcga gttgcgctac acgcggcagg
     7681 atagtgcttt tacgcaggat aaaaagagaa tattccggga gcaggctcaa tgaacgcccg
     7741 cgctcggcgg cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac
     7801 acaacaccca ctttagcctg ctctgcctgc gagatattct cggcaccttt cgccggatcc
     7861 caaacaacgg cattgcattc ctgctgcaaa cgttcgctaa tccccaattc ctccagcctc
     7921 gtgtcaccgc taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct
     7981 tctgccgcct tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac
     8041 tgaataaacg cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag
     8101 ttgttaagcg gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga
     8161 gcaacgttat tcaaaaattc gcctcgatta tccattcttt ttctcctgcg cctgatgttt
     8221 cttaaaccaa ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca
     8281 gtcgctaatc gcgccaaatt tgagtggtgt tttgccgcca ttgataaacc agcttgccgc
     8341 atgagcaccg gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa
     8401 catttttatc gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg
     8461 atgacgcaaa atcagttttg acagcggaat acgcaccgga cacacgttgt cacaagctgt
     8521 gcataaagag caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga
     8581 aatcaccgca ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg
     8641 atatgccgga caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa
     8701 ttcagaggcc agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg
     8761 accatcaacg tgcccagctt cgcgcggtcc tgtcagccag gtgttgtatc ccgtcaaacg
     8821 tgcaccaacg gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg
     8881 ggcaatacgc tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg
     8941 cgcattacct tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca
     9001 gccggtaata cctatttcag cactgaggaa atcttcgcgg attttttgcc ggatgaataa
     9061 ggtcatcgct tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg
     9121 gatctgatgg cgatctttat gaattgccgg gaccacaaca tgagatggcg gatcttgatc
     9181 cagctggaga atatattcac ccagatcggt ttcaatcacc tgaatgccag catcctgcaa
     9241 cacatgattg acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc
     9301 atttttgcgt tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg ttcttgcaaa
     9361 atagacgtga ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag
     9421 attactcaga acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc
     9481 caattcatcg accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc
     9541 tttgcgcatg atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact
     9601 ggttttgatc gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca
     9661 ctttgacttt ctgcccttcc cgttgtaatc gcccactgat gtttagcagg caactcacgt
     9721 cagcaccaat taaatactca gggcggactt ccatcaggtg cgcaaccttt tctttcacca
     9781 tctcgccgga tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat
     9841 cctgttcagc aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct
     9901 cgtccttcac tcccagctta cgggccaggc tacaagatgg gtgatacacc gctctccctt
     9961 gcaaactggc acctacatcg actaccccta atttattaac aataaaagag gtgagatcct
    10021 gcatacgcgc ggcaaccttt gcggcacgtg atgcccattc aggttcatcc gccagataca
    10081 tcgggtaact ttttacggca taggtgcagg agccagccgg tgaaataatg ggatcatcgt
    10141 tatcctccag cgcggcgatc agatttttca tccctggaat cgcttctttg atataaccgc
    10201 tattgatcgc aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga
    10261 gtttttccag tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac
    10321 aggtgacaaa gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc
    10381 cgatgtttcc cccccaccgg gggatcaatc atcaataaat atggcgttct tttttaattg
    10441 gctagcaaca taccacccct catttgtatg acaaatgata atctttcatc caaacgtcaa
    10501 atgcaataac taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgat
    10561 catttttggc ataagaaatc catttaaaaa tggctacatc ccttatacct attcagagct
    10621 acttattcag gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa
    10681 tcacgtatcg agtaatagtc ggcattttat ctaattgaag caatttagtt tgcattttta
    10741 tttaaaaact gagctagagt ccatcagaaa aaatatataa agcccaaacc accgttggaa
    10801 atagaaatag ttgaaaggct cacaactccc ggaatgcgga ttatctttgc cctgatttct
    10861 ggcggtttta ttcagggtgc aagctgtctg accctttccc gatattctcc cggggtacaa
    10921 ccaaactcgc ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc tactgactca
    10981 gcgatcacca caacagggag cgtttcccgg gaaaacatct gggccgctat ttgtagacgc
    11041 aactttgtta atacagccag cggcgtggtt ccggaaacat cacggaaaag ctgggcaaaa
    11101 cttgcccggg acatgtgagc gatgctggcc agcgattcga cggtccaggc gtgtcctggc
    11161 atttccagca tttgctgtat taccgcacca agacgtggat gcagaagcaa actgagaatg
    11221 tttttctcag tattaacctg tgcaatccac tcacgcaccg cgagggtaaa gaatgtagcg
    11281 cagatctggc tacacagtgc atccactccc ggcattgccg atctggattc ctgttgtaaa
    11341 aacgggatcg cctccctcag ccagttatat tccacgctgt ggttaaccgg tgctaaaaac
    11401 agcgtttccg gcaaagacgt taaaaaataa cgcgccgaat gttgcaaccg aagagtgccg
    11461 cagacaatac aggtcgattc attatcgaca tgactcaggc gatgagctga attttgtggt
    11521 agcaggacca catttcccgg gcgtaatgta aaaatctccc ccgtcggcat ttccagcttc
    11581 gccgctcctt gcgttaacgc atgccaacga ataaccgata attccccggc accatgtgga
    11641 agctgccagt cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg agcgttaagc
    11701 atcaacaaac ggctaagggc atccatatca gactcctgag tggtttttga ttacaaacac
    11761 caacaatctt agtaaataac taatctaata ccatcaaata gttaatcatg atttttgttt
    11821 tgtgattctg attaagaaaa agaaaatatc agacgataag acaaaatatc aagcgttgtg
    11881 aagaaatgtt atttgctctt ttgcgtctat agtcatgatg tcaaatgaac gcgtttcgac
    11941 aggaaatcat catgaataaa tatcaggcag tgattattgg ttttggcaag gctggaaaaa
    12001 cattagccgt cacgctggca aaagcaggtt ggcgagtggc tctcatcgaa caatcaaatg
    12061 caatgtatgg cgggacctgt attaatatcg gctgcatccc aaccaaaaca ttggttcatg
    12121 acgcacagca gcacacagat tttgtccgtg ccatacagcg taaaaatgaa gtggttaatt
    12181 ttttacgtaa taagaatttt cataatcttg cggatatgcc caatatcgac gtgatcgacg
    12241 gccaggcgga gtttatcaat aatcatagcc tgcgtgttca tcggcctgag ggaaatctgg
    12301 aaattcatgg cgagaaaatt tttattaata ccggtgcaca aaccgtggtt ccgccaattc
    12361 ctggaattac caccacgcca ggagtatatg acagcaccgg attacttaat ctaaaagaat
    12421 tgcctgggca tttaggtatt ttgggcggcg gatatattgg cgttgagttc gcctctatgt
    12481 tcgctaattt tggcagcaag gtaaccattt tagaagcagc ttcgctgttt ttgcctcggg
    12541 aagatcggga tattgctgat aatatcgcga cgattttacg cgatcagggc gtcgatatta
    12601 tcctcaatgc ccatgtggag cgaatcagtc accatgaaaa tcaagtgcaa gtgcatagcg
    12661 agcacgccca actggcggtg gatgcactgt taatagcttc cggtcgtcaa ccggctaccg
    12721 cttcgttaca tccagaaaat gccggtatcg cagtaaacga gcgcggggca attgtcgttg
    12781 acaagcgatt acataccacc gcagacaata tttgggcgat gggagatgtt accggcgggc
    12841 tgcaatttac ttacatatca ctggatgatt accgcattgt acgtgatgag ttactgggtg
    12901 aaggcaaacg tagtactgat gatcggaaaa atgtgcctta ttccgtattt atgacaccgc
    12961 ccctgtccag ggttggtatg acagaagaac aagccagaga gagtggtgct gatattcagg
    13021 tggtgacatt gcctgtagct gcaattccgc gtgccagagt gatgaatgat actcgtgggg
    13081 tattaaaagc gattgttgat aataaaaccc aacgtatgtt aggggcatca ctgctgtgtg
    13141 ttgactccca cgagatgatc aatatagtga aaatggtgat ggatgccggg ctgccttata
    13201 gcatattacg cgatcagata tttactcatc cgtcgatgag cgaatcactc aatgatctat
    13261 tttcattagt caaataaact caaaatcaga cgccagaaca aatattctgg cgtctcagag
    13321 aaaagaatct tattaatcct ctgttactct ataacttctt aatcacttca ttgatggtat
    13381 tttatatgtt taaaaaatct gttttatttg caacactatt atctggcgtt atggcatttt
    13441 ccaccaatgc agatgataaa ataattctga aacatatcag cgtctcgtca gtatcagcat
    13501 caccgacagt tctggaggat accattgctg atatagccag aaaatataat gcttcatcct
    13561 ggaaagtcac atcgatgcga attgataata attcaaccgc aacagcagta ttgtataaat
    13621 aaggatgttc acaatggaaa aatacctgca cctgttaagt cggggagata aaattggcct
    13681 gacattgatt cgtctgagta ttgcaattgt ttttatgtgg attgggttat taaagtttgt
    13741 cccttacgag gcagacagca ttacaccatt cgtcgcaaac agtccactaa tgtcgttctt
    13801 ttatgaacac ccggaagact ataaacagta tctgactcac gaaggcgaat acaaaccaga
    13861 agcaagggca tggcaaacgg ccaataatac ctatggtttt tccaacggtc ttggcgtcgt
    13921 ggaggtgatt attgctctgc tggttttggc taatcctgtc aatcgctggt taggtttatt
    13981 gggaggactg atggcattta ccacgccgct ggtaacactc tcatttttaa tcaccacccc
    14041 ggaggcatgg gtacccgcat tgggtgacgc tcatcatggt ttcccttatt tatccggtgc
    14101 tggtcgcctg gtattgaaag atactctgat gctggcaggt gctgtcatga taatggcaga
    14161 ttcggcgcgg gaaattctta aacaacgcag taatgaatcc agttcaacgt taaaaactga
    14221 atattgataa tccgaacact gtctgttgtt cactttttca ggcgtcaggc cccctttctg
    14281 atgatgaagc ctgacgccat attaccggag atatgacaga gtgaagatca atatcactcc
    14341 cctgttaaaa ggttattgtc gtcttaggta aaatacatga ttaatatcaa tcatgtattt
    14401 ttacagcaag acaaccacga acaaattcag gattaaaatg ttttaccgac tccccaacat
    14461 aacccaaatc aagcgaacta atttgtgcca tttctttatc tgtcagtgag aaatcaattt
    14521 ttgatgggca tctgcaatac tctgaagcag actatacgga aaattccact gtcagtccct
    14581 ccattaggca tgaacaatga gtctacgtta aaacgtaacc tcaaagtagt atgtggattt
    14641 tgatatcact tatgcaaaaa attcattaat aatgtaggac tgaaacctct ctattttcgg
    14701 ggacaacgaa gcagacgcta ccagtgcttt tgccttcgcc cttgctattt ttgatacact
    14761 tagggcccag ggtataacga aaatgtgcga tatgacaaag aattaacgga gaatgagatg
    14821 atcaggcaga agattctaca gcagctcctg gagtggattg agtgcaatct tgagcaccct
    14881 atttcaatcg aagatatcgc acagaaatct ggctacagca gacgcaacat ccagcttctg
    14941 ttccgaaatt tcatgcatgt gcctttggga gaatacattc gcaaacgaag gctttgtcgt
    15001 gccgccattc ttgtccggct caccgcgaaa tctatgcttg atattgcact ctctttgcat
    15061 tttgattcac agcagtcatt cagccgcgaa tttaaaaagt tattcggctg ctctccccgt
    15121 gaataccgcc accgtgatta ttgggatctc gcaaatatct tcccttcttt tttaatacgt
    15181 caacagcaaa agacggagtg tagattaatc aactttcctg agacacctat ttttggcaac
    15241 tcatttaaat atgacattga agtgtcgaat aaatcaccgg atgaagaagt caaactacga
    15301 cgtcatcatt tagccagatg tatgaagaat tttaagacgg atatctattt cgtttccacg
    15361 tttgaaccgt caacaaaatc ggtcgatttg ctcacggttg aaacttttgc tggtacggta
    15421 tgtgaatatg ctgacatgcc aaaagagtgg acaacgaccc gaggacttta tgcctctttc
    15481 cgttatgaag gaaactggga aaattatcct gactgggtgc gtaacatcta tctgatagag
    15541 ttacctgcca gggggttagc cagagtgaac ggcagcgata ttgagcgctt ttattacaat
    15601 gaagatttcg tagaaaagga tggcaatgat gttgtttgcg aaatttttat tcccgttcgt
    15661 ccggtttagt tggtcactat ctcttattga gttttatcct tgcctgatac tatttaatca
    15721 gtatcaggca aggtattcag tgaataatgg cgttgaatat ttcaacacca ttattcttta
    15781 ttagatcgta actttcatac tattcaaatt atggcatctc ctcctcgcca ttttcagtct
    15841 ctgcagcaac ggcgttaatt gtcaccttca caggcgtcat ttcgtacccg ccatacgtca
    15901 gtgcgttgaa tgtgaacgta tagacacctg gcttgctggt actaaatgta cgcgtgattt
    15961 tcccacctga gaaatggtca gccttcgacg gcaggaattg ataatccttc tccgtcactc
    16021 cttccggagc ctcaatgtcg acccacatac ttcctcctac gggattgact cccgccatca
    16081 gggtaaccgt cattgttgca gtagtcttcc cgtccgccac cagagaatct acacgttctg
    16141 gagatgcggc gagcaccgcg tttttatcat ccgcgacgaa tttagattca gcagttttcg
    16201 tcgatacctg accgttggcc tgttccactt tcgccgtgag tgtatatgtc gctgcgatag
    16261 tggtagagcc ggtgaaaaca gcctgtcctt gctcattagt tttcgccgtc cctttaggat
    16321 ccagaactaa attttccgag ctggcagtca gcgtcacgtc gctatccttc aacaggttgt
    16381 tattggcatc agtcaccgta actttatacg tctgtttggt tttgccatcc gccactgcac
    16441 ggttgccaat cacctcaata ctggagatgg tcgccgtagt cttatcggcc acaaacgtta
    16501 cagactgcgc attcgaaggc tggtcatttc ctgccgacgc cgtgatctca tacgttccgg
    16561 cagtcacgga gaccactttc agttccgctt taccctcctt gtcggcgttc accatgatat
    16621 taccgtctgc aagcggtttg acgccccgag gcagattaaa gacgaccaca gcgccaggca
    16681 gaaggttgcc gtattgatcc ttaaccgttg ccgtcagcgt aaaggcatca ttgtcgtttg
    16741 ccacttttgg cgtgctgcca tcaacctcca gggtcgcctg accggtactg aaatccgcct
    16801 tgaatttcat cgtaaccgtc ttctgagcat tattcactga ggccgtgatg ctgtgttccc
    16861 ccgcaaccgt tgacataagc tcaatgtcca ctttccctgc cgcattggtt gttactgtat
    16921 tccccgtctt gctggtcaca ccctgcggta aagtcagcgt aacttcttgc ccctgcaacg
    16981 ggttaccata gctgtccacg acggtcaggg ttatctggtt agcagactgt ccattagcca
    17041 gttggttatt aaccttcact gtcatatcac gaatctcagc cttagatgcg tcacctgcaa
    17101 cgttcagcac cagtggctga gcaacggcat tttggccgtt cactcgcggc atcacagaca
    17161 actgacccgc ggcagatccc agcgttaagg tcgccacgta gaccccatta cctttctctg
    17221 tccagtttcc tgctgaagga cgctcactcc ccgtgctggc ggcaccgcta aacactggtg
    17281 catctggctt cagcccggtg accgggttcc cgtacgcatc cttcacggtg aaggtgagtt
    17341 ccgtcgtcat tttgacggtc ggagccttat tgtccgcaac aagcgtagag ttcgctgatg
    17401 acatctctcc ggcgatgacc gtcaactgcg cggcttcggt ggctgctggc tggccgttga
    17461 agagcggcat gacacgaagc tcgcccgtct ttccgcctgt agttaacgta gcaacatagg
    17521 aaccgtcacc tttttcggtc caactggaaa cggtcgcccc ttcagaggcg gtccccgtca
    17581 aacttgccga caacgaaaga ccactgatag tgttgccatg tgcatctttt gcaataagcg
    17641 tcacggttgt gctttcgccg gcttttacgt gatcttctgc gacagagact ttcgactggt
    17701 ttgaagataa cgcatcagcc accacggtta cttttgccgc atttgctgcc gcatcctgtc
    17761 catttagctt cggcataact tctaattcac ccgccgtaga gccgagagta atctgcgcag
    17821 tccacgtccc atcaccatta tttgtccagc cagatgccgt agaacctaca gcagcggcac
    17881 ccgctaatga cggcgcttcc ggcgtgaggc tggtcacagg gttgtcatag gcatcttttg
    17941 ccgtccagat tgccgtaact gtaccgccaa ccaccggttt atcaggattc agggtgatgg
    18001 acgaatgtgc tgcatcaagc ggaccggcaa caaacttcag cgtttgttgt aatgtgccca
    18061 gctgatactg ctcgctgaaa gcacgcacaa caacgtttcc ggcacgggtc gaagaaaccg
    18121 tggcgctgta aacgcctggt tttatttccg aaatggcacc aacggttaca ccattagtgt
    18181 cttgcggaac aaatcgcaag cggctggctt ctcccgtcac cggattaccc tcggagtcca
    18241 ccgcagtcaa cgtcaacgta taggcttgct gaccatcagc aaccacatca cctgacggct
    18301 cgttagcgct cagggtggag tttgccacat ccatcatcgt ggcccgcagt tctgcagtga
    18361 cggttttgct catgccatca acgctaacag taatcgttgc ctcacctgac tgcgttccgg
    18421 tagtaaagac agactgatac acccctgctt cagtttcggt gaactcaccc agtgttggct
    18481 ttgcctgtga tttagtggcc ttcagggaac gagtcacaat atttccagcc ggtttgaagg
    18541 ctagttcagt cttgatctga tctttcatgc ccgtgactgg ctgcccctcg gcgtcgcgca
    18601 gagacagcac cagcggtctt tgctcattac cgttagcaag catttgaata cggctctgac
    18661 cgtcaagcgt taacgccgtg cgatcggcgc tcatacctgc tccggtaatg accacctctg
    18721 tctgcacgcg ttttgaggca ttgcctttgt tatcgtaggc aaccgcagaa atcgcataat
    18781 aattgtcttt gcctggacga taagccggga gcgttacttg ccactgacta ccctgaccgg
    18841 taattttgcc accctcagcc agtaatgacg gcgcttccca ctgcacattt ttcagtccgt
    18901 gagttgcttt gctgaccaca agccccaggg aaagtgtctg accaccctta ccttcaatac
    18961 gttcaggcag agcaatacgg atcacttcag atttgcggta ctcaagaacg atgttgttat
    19021 tacgctcaac caggtcatag cggctgcctg ccagtacccg acgctcgcga atgctgtctg
    19081 tatcgagttg tttcgccaga ggttcgccaa tccgataatt aacttccagg ccaaagcgag
    19141 tgtcattctc accactcttg ccctgcttat gcccggcgct cagtgtcaga agaggcactg
    19201 gcgtataggt cacctcggca gaaatagcat gcgggtcttt ctggcgctta tctttaccaa
    19261 acagcccgac ttcatcgcca taatactgtt catacatcag gcttgcgcca agctgcggcc
    19321 aggcgggtaa atagccctca gcacgaatat cccagccatt cgccgggcgt tcctgataat
    19381 cctcaacatc cggcgatttt ttccagccag aagcccggat ataaccattg gcgctcagtt
    19441 tcagataatc gcgccagtat tccgcaccaa caccaatgcg ggtatgacta cgggataaat
    19501 catggtcgat aaaggtgttc acccccgcca tccagtcatt tcctgaaaaa tgacgccagc
    19561 caaaaccaat atttgactga gtacgatcgt cggtacgatg tattgctccc tgagtgaaca
    19621 acatatttgt tggcgtatca taaatcggat aaagcatttc cagcgaagaa tccttcagcg
    19681 agaaatcttt atcgacattc agtttgacgc gcgcagtacc atatttcccg agccactcct
    19741 gtatttcctg gttagcttta gctgtggcca ttccggtaat aaagttacgt gtcgcatcgc
    19801 tatctggctg actgcttaaa aatgtcccgg catttgcggc aaacgacgcg acatttttct
    19861 ccacgttatt atcagcagtt accgtagtat ttcccatgct caaccgtggc tgaaccgcat
    19921 gctgcgcacg tgccgccatt actggggtaa aggtgacagc gagtggaaaa agaacctgaa
    19981 cagagatatt tgcccacgcc acgcagcggg ccagaactga ataacgaaat cgtggttgtt
    20041 tatgacctgt tttataatgt gacattgaga taatttcctg atgaacgaat taacgaacat
    20101 aggcagacac aaataatggc gtctgcttgt cagaaaaacc ggggatttcc cgatttcctt
    20161 ttccgcagaa aaggaaatac ctttatgtaa gaaagtgcac cgccacctaa tatatccagt
    20221 gcctgtagcc actcatcagg tggttgggcg cgggattata tttttattta tgggggggga
    20281 aatttagctt tttgtgcaag tcattaaata gatacctaag ttatatttct tactctgcgg
    20341 acagtgaata attcactatt cagagtgaat aacaatgacc tgcctttaat taaaactatt
    20401 ggaaatacgt cgaaataggt atatatacca ggataaacca atatattttt tatggatatt
    20461 cattttcctt catcggactg taaattgcgg agatatcgaa cttgcactac ccatcgcaac
    20521 actcaaacag atttatgaat ttcgttcata acccctgaca aattctgccg tctaatcgag
    20581 cttttccctt tacgttatgc ttctcatgaa cgataacaca acttgttcat gaattaacca
    20641 ttccggataa actatgggat caaattcagg catcgaaagg aactggctgg tttccgttaa
    20701 tggattaaat atctattatt ctcccgagag taaaactatc aaatcaatga gtaaccgata
    20761 cagcctcaca aattctttca gataattaac ccgtcactgc ggcacgttct ctcataaggc
    20821 gtgcctgcag tgtaatccag tatgtatccg gcatagttga cggtttaata gaactcaatt
    20881 cctctcccag gtcaccaaca acgatatctt gtataacacc ccaggagata cgacattatg
    20941 atgagatgcg atatgctcct cagcaccaat caacgcccgg actcggccag tcttgagatc
    21001 agactcattc ccggaactga cttcaggcga catatatcaa tgacgttggt gcaataaaag
    21061 atagttgatg taatacatct attcaaattt gtgatacatg tcaaaaaatt ggttgaccaa
    21121 actcgttatt tatataaggg cacttacgaa gtgcactctt ttttaaagcg aggaagtacc
    21181 aatgaaagag aataaagtac agcaaatcag tcataaactg attaatatcg ttgtttttgt
    21241 cgcaattgta gaatacgcct atttatttct ccatttccat taataacgga aataaactgt
    21301 tcacttcagt gatatttaaa atatgcatcc tctccctttt ttgtaagtaa ttattatatc
    21361 cgtgggagag gaatacacgc tgtcaggtaa tcaatcatac tgcgataaat catcggccag
    21421 taaagtggag ataacctcca ttctcgaaaa atccatactc tcagcaaaac catcatcaat
    21481 cactcatcca ggcgtttatg ggagcgtcac caatggctgc taacaatgcc agagttcccc
    21541 gttgcgaaaa ttccacatcc acaaagagtc acagggattg agtgttgaaa tgatccggat
    21601 gagcatgtat ctttatggtt atgttataac ataacaggta aaaatgatga agcccaatat
    21661 ccatcctgag tatcgtactg tggtgttcca cgacaccagt gttgatgagt actttaaaat
    21721 cggctcgact atcaaaacag accgtgagat tgagctggat ggcgtaacgt atccatacgt
    21781 gacaattgat gtctcttcta aatcgcaccc gttctataca gggaagctga gaacagtggc
    21841 atcagaagga aatgttgcac gattcaccca acgttttggt cgttttgtta gcacgaaaaa
    21901 gggggcgtga tgaaagttct taactctctg cgtaccgcaa aagaacgcca tccagactgt
    21961 cagattgtga agcgaaaagg acggctatat gtgatttgta aatctaatcc acgttttaag
    22021 gccgttcagg gtcgtaagaa aaaacgttga ttcaaaattc gacggattaa cgatatttgt
    22081 ctgattaata atcagatcgg attaatgttg gtgtgtttat aacaccaaca ttaattttcc
    22141 tggggatata ttcttcctgt tcatttgagg ccaactgcct gacgtttctc tccgaatatt
    22201 ccattatctt aatgttgact tgttgaccag cttcgcccct gta
//
LOCUS       DACAPB010000065        22171 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.065, whole genome shotgun sequence.
ACCESSION   DACAPB010000065 DACAPB010000000
VERSION     DACAPB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.065"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1772)
                     /gene="alaS"
                     /locus_tag="IT904_002205"
     CDS             complement(<1..1772)
                     /gene="alaS"
                     /locus_tag="IT904_002205"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP3191979.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVN"
     gene            complement(1900..2400)
                     /gene="recX"
                     /locus_tag="IT904_002206"
     CDS             complement(1900..2400)
                     /gene="recX"
                     /locus_tag="IT904_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP3191980.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEI
                     WRNFAD"
     gene            complement(2469..3530)
                     /gene="recA"
                     /locus_tag="IT904_002207"
     CDS             complement(2469..3530)
                     /gene="recA"
                     /locus_tag="IT904_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP3191981.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(3610..4107)
                     /gene="pncC"
                     /locus_tag="IT904_002208"
     CDS             complement(3610..4107)
                     /gene="pncC"
                     /locus_tag="IT904_002208"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAP3191982.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFSTARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(4252..5337)
                     /gene="mltB"
                     /locus_tag="IT904_002209"
     CDS             complement(4252..5337)
                     /gene="mltB"
                     /locus_tag="IT904_002209"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAP3191983.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYS
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            5593..6156
                     /gene="srlA"
                     /locus_tag="IT904_002210"
                     /gene_synonym="gutA"
     CDS             5593..6156
                     /gene="srlA"
                     /locus_tag="IT904_002210"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP3191984.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            6153..7112
                     /gene="srlE"
                     /locus_tag="IT904_002211"
                     /gene_synonym="gutE"
     CDS             6153..7112
                     /gene="srlE"
                     /locus_tag="IT904_002211"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP3191985.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGIAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPHPSSVGRDYDTSKKITEQSDGLLAKVGMSMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            7123..7494
                     /gene="srlB"
                     /locus_tag="IT904_002212"
                     /gene_synonym="gutB"
     CDS             7123..7494
                     /gene="srlB"
                     /locus_tag="IT904_002212"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP3191986.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            7498..8277
                     /gene="srlD"
                     /locus_tag="IT904_002213"
     CDS             7498..8277
                     /gene="srlD"
                     /locus_tag="IT904_002213"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAP3191987.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            8383..8742
                     /gene="gutM"
                     /locus_tag="IT904_002214"
     CDS             8383..8742
                     /gene="gutM"
                     /locus_tag="IT904_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAP3191988.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTIFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            8809..9582
                     /gene="srlR"
                     /locus_tag="IT904_002215"
                     /gene_synonym="gutR"
     CDS             8809..9582
                     /gene="srlR"
                     /locus_tag="IT904_002215"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAP3191989.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            9575..10540
                     /gene="gutQ"
                     /locus_tag="IT904_002216"
     CDS             9575..10540
                     /gene="gutQ"
                     /locus_tag="IT904_002216"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAP3191990.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(10537..12051)
                     /gene="norR"
                     /locus_tag="IT904_002217"
     CDS             complement(10537..12051)
                     /gene="norR"
                     /locus_tag="IT904_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAP3191991.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRCLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            12238..13677
                     /gene="norV"
                     /locus_tag="IT904_002218"
     CDS             12238..13677
                     /gene="norV"
                     /locus_tag="IT904_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAP3191992.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNSVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            13674..14807
                     /gene="norW"
                     /locus_tag="IT904_002219"
     CDS             13674..14807
                     /gene="norW"
                     /locus_tag="IT904_002219"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAP3191993.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DISHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(14935..17187)
                     /gene="hypF"
                     /locus_tag="IT904_002220"
     CDS             complement(14935..17187)
                     /gene="hypF"
                     /locus_tag="IT904_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAP3191994.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLDLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAT
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(17340..17867)
                     /gene="hydN"
                     /locus_tag="IT904_002221"
     CDS             complement(17340..17867)
                     /gene="hydN"
                     /locus_tag="IT904_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAP3191995.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(18016..19029)
                     /locus_tag="IT904_002222"
     CDS             complement(18016..19029)
                     /locus_tag="IT904_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP3191996.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     GYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSV
                     WCDHKQTSFNAVAELINARHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIA
                     PSR"
     gene            19286..20743
                     /gene="ascF"
                     /locus_tag="IT904_002223"
     CDS             19286..20743
                     /gene="ascF"
                     /locus_tag="IT904_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAP3191997.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDVLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            20752..>22171
                     /locus_tag="IT904_002224"
     CDS             20752..>22171
                     /locus_tag="IT904_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP3191998.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPV
                     YSAHVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     INYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDL"
BASE COUNT         5163 a         6103 c         5772 g         5133 t
ORIGIN      
        1 tcgttaacca gtgaaccttt ctgcgataca tgagtaccca gaacctggcg cagcgcagcg
       61 tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc ctcatcaaca
      121 tcagcctgca ccgcgtcgcc cactttcaga gaacccgcag caagtttacc gatgtgacca
      181 atcgcctggc cgtatttctg cgtatcttcc accgcaaagg agaagttagc gcctttcagt
      241 tcgcctttat cgccaacctg accgccggat tccgcataga atggcgtttg atccagcacg
      301 accacagctt cctggcctgc attgatggca tcaaccgctt taccatcaac aaacagcgca
      361 gtcactttgc cgttcagttc cagatggtca tagcctttaa attcagatgc actgtcaaca
      421 cggatcattg cgttgtaatc ggcaccaaag ccgctggctt cgcgcgcgcg acgacgctgc
      481 tcttccattg cagcttcaaa accagcttcg tcaactttga tgttgcgctc acgacaaaca
      541 tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca gacggaaagc agtttcacca
      601 tccagcgtat caccagaaag ttttgccagc tcttcatcca gcaacgccag accgcgctcc
      661 agagtacgag caaactgctc ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg
      721 cgtttcaggt cttcacccgc agagcccata acgtcgatca gcggaccaac cagtttgtag
      781 aagaaggttt ctttcgcgcc gagcatatta ccgtgacgca ctgcgcgacg aatgatacga
      841 cgcagtacat aaccacggtt ttcattggac ggcattacgc catccgcgat caggaacgca
      901 caagaacgaa tgtggtcagc gattacgcgc agcgatttat tgctcagatc ggttgcgcca
      961 gtgactttcg ctaccgcctg gatcagcgtg cggaacaggt cgatgtcata gttagagtta
     1021 acgtgttgca gcaccgcagc aatacgctcc agacccatac cggtatctac agacggcttc
     1081 ggcagcggtt ccatcgtgcc atcggcctgg cggttgaact gcatgaagac gatgttccag
     1141 atctcaatgt agcggtcgcc gtcttcttcc gggcttcccg gagggccccc ccaaatgtgg
     1201 tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc acccatctgc
     1261 cagaagttgt cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg
     1321 cgcgggatcc ctacttcttt ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg
     1381 gtaacccaca gacgctcttt cggcagggca aaccattttt cgctggtcag cagttcccat
     1441 gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg
     1501 aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca ggtcgttgtg tttaccaccc
     1561 gcacgcacgc agcgttggga agtggtagcg cgggaataat tacgcttgtc gagcccaagg
     1621 aacacatcct tgaactggtt catcccggcg ttggtaaaca acaaagttgg gtcgttatgg
     1681 ggtaccaggg agctgctggc aactacctga tgtcccttac tatggaaaaa gtcgagaaac
     1741 gcctgacgga tctcagcggt gctcttgctc ataattatcc tgaaatcaag ctaacgaaat
     1801 atcgccacca gctccagcgt gtcttaaccg ccgggctggt aactgaaaag tgggaataag
     1861 ataagttttc ttgactggga agtaaaatac cgtatgcgtt cagtcggcaa aatttcgcca
     1921 aatctcctgg atatcttcca tcagatagcc acgatagagc agaaaacgct ggatcttaac
     1981 tttttctgaa aagacagttg gcaaaggttc gccatatttt cgcgtcgcct gatcgcgcgc
     2041 cagtgcgcac cagtcgatgt cacattcacg catcgctttt tctgtcgctt cgcgggaaat
     2101 acctttctga ttcagttcct ggcgaatacg cgcaggtcca taacctttgc ggctacggct
     2161 ggcgataaag cgcgcaacaa atcggctgtc atcgagatag ccatgttcat ggcaccaggc
     2221 aataacgcgc tcgtaatctt ctgccgtagc atcaatctct tctgggccat ttttgcccat
     2281 aatcggtgcc gcgagtttac gtcgcagttc ttgctcactg tgatcgcgca ccgccagaat
     2341 gcgtaccgca cgatccaaca ggcgagcata tgccgggcga cgggatgttg attctgtcat
     2401 ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga tgcgaccctt gtgtatcaaa
     2461 caagacgatt aaaaatcttc gttagtttct gctacgcctt cgctatcatc tacagagaaa
     2521 tccggcgttg agttcgggtt gctcagcagc aactcacgta ctttcttctc gatctctttc
     2581 gcggtttccg ggttatcttt cagccaggca gtcgcattcg ctttaccctg accgatcttc
     2641 tcacctttgt agctgtacca cgcgcctgct ttctcgatca gcttctcttt tacgcccagg
     2701 tcaaccagtt cgccgtagaa gttgatacct tcgccgtaga ggatctggaa ttcagcctgt
     2761 ttaaacggcg cagcgatttt gttcttcacc actttcacgc gggtttcgct acccaccacg
     2821 ttttcgccct ctttcaccgc gccgatacga cggatgtcga gacgaacaga ggcgtagaat
     2881 ttcagcgcgt taccaccggt agtggtttcc gggttaccga acatcacacc aattttcata
     2941 cggatctggt tgatgaagat cagcagcgtg ttggactgct tcaggttacc cgccagctta
     3001 cgcatcgcct ggctcatcat acgtgccgca aggcccatgt gagagtcgcc gatttcgcct
     3061 tcgatttccg ctttcggcgt cagtgccgcc acggagtcaa cgacgataac gtctactgcg
     3121 ccagaacgcg ccagggcgtc acagatttcc agtgcctgct cgccggtgtc cggctgggag
     3181 cacagcaggt tatcgatatc gacgcccagt ttacgtgcgt agattgggtc cagcgcgtgt
     3241 tcagcatcga taaacgcaca ggttttacct tcacgctgcg ctgcggcgat cacctgcagc
     3301 gtcagcgtgg ttttaccgga agattccggt ccgtagattt cgacgatacg gcccatcggc
     3361 agaccacctg ccccaagcgc gatatccagt gaaagcgaac cggtagagat ggtttccaca
     3421 tccatggaac ggtcttcacc caggcgcatg atggagcctt taccaaattg tttctcaatc
     3481 tggcccagtg ctgccgccaa cgctttctgt ttgttttcgt cgatagccat ttttactcct
     3541 gtcatgccgg gtaataccgg atagtcaata tgttctgttg aagcaattat actgtatgct
     3601 catacagtat caagtgtttt gtagaaattg ttgccacaag gtctgcaatg catacgcagt
     3661 agcctgacga cgcaccgcat cacggtcgcc gctgaagcat tcccgccggg taatgccttc
     3721 accgcgggca gtggaaaaag caaaccagac ggtgccgaca ggcttctctt cactgccgcc
     3781 atccggcccg gcgataccac taatagacac ggcataatca gcacgagccg ctttcagtgc
     3841 gcctatcgcc atttccacca cgacgggttc actcaccgcg ccatgctgcg ccagcgtctc
     3901 ttcgcgtacg ccgatcatct gcgctttggc ttcgttactg taggtgacaa atccgcgttc
     3961 aaaccaggcg gagctaccgg caatatcggt aatcactttc gctacccaac caccggtaca
     4021 agactcggca gttgttacgg ttgcgccacg ggctttcagc gcctgcccaa cctgttcact
     4081 taactgcatc agttcactgt cagtcatcac aacctctgtc agtcaaaaat ttatgccgga
     4141 caagatagca ctttcgtcag gaagatggtg ttggcgttgc aattttacga ggaggattca
     4201 gaaaaaagct gattagccag agggaagctc acgcccccct cttgtaaata gctactgtac
     4261 tcgcgccagc gccacggctt gtcctaactg ccagaccgcc attgcgtaat gggtgctgtg
     4321 gttgtaacgg gtgatgctgt agaagttcgg cagaccgtac cagtactggt agccggtgcc
     4381 aacatccaga cgcagcaggc tggcttgttg atggttgccc agcggctgct gtggcgttaa
     4441 acctgcggcg gcaagctgcg aaatgctgta cttagttttg aagccatttg gcaagcctgg
     4501 agcctgaccg tttgccatta ccgcgacctg atcgcctttc acccagccgt gcgctttgaa
     4561 atagttcgcc acgctaccga tcgcatcaac cggatcccac aggttgatat gcccgtcgcc
     4621 gctgaaatct accgcatatt gtttgtaaga cgacggcata aactgtccgt agcccatcgc
     4681 cccggcaaag gaacctttca gattgagcgg atcgtcctgc tcgtcgcgcg ccatcaacag
     4741 gaaggtttcc agctcgccag agaaatactc cgcgcggcgt gggtagttaa atgacagcgt
     4801 tgccagcgca tcgaggatgc gagttttccc catcacgcgc ccccagcggg tttcaacgcc
     4861 gataatcccg acgataattt ccggcggtac tccatacacc tgccacgcgc gattcaacgc
     4921 atcttcatac tgattccaga aaaccacacc gttctgcacg ttgtccggcg taataaattt
     4981 tttgcgataa cggagccatg cgccgttcgg acctgatggc ggtttcaccg atgtggttgg
     5041 tgcctggtta tccatcagcc gcagtaccga atccagacgc ttcgcctggg agagaatttc
     5101 ctgcaactgc tgacgatcga aaccgtgttt gttcaccatt ctgtcgatga actgctgggc
     5161 attcgggtta ttagcgaaat cgccgcccat ctgcatcaca ttgtgctgcg gctcaagcag
     5221 gaagccgcca gacggcgttc cggtggtcgt atcagtctca gtaggttttg gcttgctgct
     5281 acaggcggca agcaacacaa aaaggggaag caatgttaca taacgacgct tgaacatgag
     5341 gggtccattt aacagattca accaggggca agtatggtaa agcatcacgc cccgcacaag
     5401 gaagcggtag tcactgcccg atacggactt tacataactc aactcattcc cctcgctatc
     5461 cttttattca aactttcaaa ttaaaatatt tatctttcat tttgcgatca aaataacact
     5521 tttaaatctt tcaatctgat tagattaggt tgccgtttgg taataaaaca ataaatcctg
     5581 aaggagagaa caatgataga aaccattact catggtgctg agtggtttat cgggctgttc
     5641 caaaagggcg gagaggtgtt taccgggatg gtgaccggca ttcttccgct gttgattagc
     5701 ctgctggtta tcatgaacgc actgattaat tttatcggtc agcatcgtat tgaacgtttt
     5761 gctcaacgtt gcgccggtaa ccctgtttcc cgttacctgc tgttaccgtg cattggcacg
     5821 tttgtctttt gcaatccgat gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa
     5881 cccagctact acgcggcggc ctcttatagc tgccactcaa tgaatggcct cttcccccat
     5941 atcaaccctg gcgaactgtt tgtttatctt ggcattgcca gcggtctgac aacgctgaac
     6001 ctgccacttg gcccactggc ggtgagttat ctgctggttg gtctggtcac caacttcttc
     6061 cgcggctggg tgaccgatct gaccaccgcc atttttgaga aaaagatggg cattcaactt
     6121 gaacaaaaag ttcacctggc aggagcaaca tcatgacgca tattcggatc gaaaaaggaa
     6181 cgggtggctg gggcggcccg cttgagctga aagccacgcc gggcaaaaaa atcgtctata
     6241 tcaccgccgg tacccggcct gcgattgttg acaaactggc acagcttact ggctggcagg
     6301 ctattgacgg atttaaagaa ggtgaacccg cggaggcgga aattggcatt gcggtaatcg
     6361 actgtggcgg cacattacgc tgcggcatct atccgaaacg gcgtattccc accattaata
     6421 tccactcgac gggcaagtcc ggtccgctgg cgcagtacat tgtggaagat atttatgtct
     6481 ctggcgtaaa agaagaaaac atcactgtag tgggtgatgc gacaccacac ccctcttccg
     6541 tgggccgtga ctatgacacc agcaagaaaa tcaccgaaca aagcgatggt ttactggcga
     6601 aggtgggaat gagtatgggt tctgccgttg ccgtgttgtt tcaatctggt cgtgacacca
     6661 tcgacactgt attaaaaacc attctgccgt ttatggcatt cgtttcggcg ctcattggca
     6721 tcattatggc ttctggcctt ggtgactgga ttgcccacgg tcttgctccg ctggcgagcc
     6781 atccactggg tctggtcatg ctggcgctca tctgctcctt cccgctgctt tcacctttcc
     6841 tcggcccagg cgcagttatc gcacaggtta tcggcgtatt gattggcgtg cagattggtc
     6901 tcggcaatat tccgccgcat ctggctttac cggcactgtt tgccatcaac gcgcaggcgg
     6961 cctgcgactt catcccggtc ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg
     7021 tcggtgtccc ttctgtactg gtgagccgct ttttaaccgg cgcaccgact gtactgatcg
     7081 cctggtttgt ctccggtttt atctatcaat agaggctgaa acatgaccgt tatttatcag
     7141 accaccatca cccgtattgg cgcgagtgcc actgacgccc tcagcgacca gatgctcatc
     7201 acctttcgtg aaggcgcgcc agcggacctc gaagagtatt gcttcattca ttgccacggc
     7261 gagttgaaag gtgcactcca tcccggtttg caatttacgc tcgggcagca tcgctatccg
     7321 gtgaccgctg ttggcagcgt ggcggaagac aaccttcgcg aactgggtca tgtcaccctg
     7381 cgcttcgatg gtttaaacga agcggaattt ccgggcactg tccatgtggc aggccctgta
     7441 cccgacgata tcgcgccggg atcggttttg aagtttgaat ctgttaagga gtaaaaaatg
     7501 aatcaggttg ccgttgtcat cggtggtggg caaaccttag gcgcgttcct gtgccacggt
     7561 ctggctgccg aggggtatcg cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat
     7621 gtggcacaag aaattaacgc cgaatatggt gaaggaacag cgtatggttt tggtgctgac
     7681 gccaccagcg agcaaagcgt tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc
     7741 gtggatttgc tggtctacag cgccggaatt gcaaaagcag cctttatcag cgacttccag
     7801 ctcggcgatt ttgaccgttc gctacaggtg aatctggtgg gttatttcct gtgtgcccgt
     7861 gaattttcgc gtttgatgat ccgcgacggg attcagggtc gcattattca gatcaactcg
     7921 aaatccggca aagtgggcag caaacacaac tctggctaca gcgcagcgaa atttggtggc
     7981 gtcgggctga ctcaatcact ggcgctggat ctggcagagt acggcattac ggtgcattca
     8041 ctgatgctgg gtaacctgct gaaatcgccg atgtttcagt cgctgttgcc acaatacgcg
     8101 accaagctgg gtatcaaacc ggatcaagtc gagcagtatt acatcgataa agtaccgctc
     8161 aaacgcggct gcgattatca ggatgtgctg aatatgctgc tgttctacgc cagtcctaag
     8221 gcgtcgtact gcactggtca gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca
     8281 acagcggaga tccgttaagg atctccgtga gactaataga atgcctgatg cgctacgctc
     8341 atcaggccta caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
     8401 actgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
     8461 tttaaccgtg ccttcgacac actatgccag caaggtcggg ttggcgtggg ccgttccagc
     8521 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gcgcatcgtc
     8581 gacaccttgt ttatgaaagg actgaccatc ttcgcccgac cgcaaaaaat tcccgcaatt
     8641 accggcatgc atgcgggtga tttacaaccc gatgtgatct ttccccatga tccactatca
     8701 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
     8761 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
     8821 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
     8881 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
     8941 catgcaggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
     9001 ccgcctatcg atcataaaac actcatcaac acccacaaga aagagctgat tgcagaagct
     9061 gccgttagtt ttattcatga tggcgactca ataattctgg atgctggcag taccgttttg
     9121 cagatggtgc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
     9181 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
     9241 tttcgtaaaa aatcggcctc atttcacggg cagctggcag agaatgcctt cgagcatttc
     9301 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacc
     9361 tttaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
     9421 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
     9481 gtcgataagc tgattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
     9541 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacgcggg
     9601 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
     9661 tgattttgtt cgcgccgcca atatcatcct gcactgtgaa ggcaaagtgg tggtttcggg
     9721 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acgcttgcca gtaccggcac
     9781 tccggctttt tttgtccatc cggcagaagc gctgcacggc gatctgggga tgatcgaaag
     9841 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
     9901 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
     9961 gctgggcctg gcggcaaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    10021 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    10081 ggcggtcatg caggcgcgcg gatttaatga agaagatttt gcccgctccc acccagccgg
    10141 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgccgtg acgatgccat
    10201 cccacaggtg gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    10261 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    10321 cgatttacgt cgctggctgg ttggcggcgg cgcactcacc acgccagtca atgaagcgat
    10381 gacggtcggc ggcaccacgt tgcaatcgca aagtcgcgcc atcgacgcca aagagatcct
    10441 gatgaagcgc aaaatcactg ccgcaccggt ggtggatgaa aacggcaaac tcaccggcgc
    10501 aataaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    10561 cgccagccga tgcaggttgg cgacgtcggt ttccagcatc cgcgcgcagg cagcccagtt
    10621 gtgatgattt tgtgccagtg cctgacgaat agtttcacgc tggaacgctt ctgtcgcttc
    10681 acgcaggttt tgcttaacaa cgggcaccgc cgccacttct ggcgtcggca acgtcacctc
    10741 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgctgcggg tggctctcgc
    10801 cagaactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    10861 gtgttgcagt aaatttcgcg ctccggcact taataccacg cgggagagcc cctgccgcaa
    10921 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    10981 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    11041 tgccagcacc tcttcgcgta aatcgcggtt agtcgccgcc agcacgcgca catcgacccg
    11101 caaacaacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    11161 ggcctgcaat gccaacgaca actcgccgat ctcatccaga aacagcgtgc cgttatccgc
    11221 catttcaaac ttcccgctgc gattactgat agcgccagta aacgctcctt tcacatgccc
    11281 gaacaactca ctttccgcca cactttccgg cagtgcagca cagttgagat agaccagcgg
    11341 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    11401 agtctcaccg ctgatcagga cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    11461 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    11521 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    11581 taacgctccc gccgccagcg cagcaatcag ccgtagctct tcgtcgctga aaacatcgaa
    11641 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgcc cggcaaacaa
    11701 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    11761 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    11821 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    11881 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    11941 ctccagcacc tgacgtagcg tggtaatcag gcgctgaaaa cgatcctggt gaccaatccc
    12001 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    12061 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    12121 actcattaat gggcataatt ttatttatag agtaaaaaca atcagataaa aaactggcac
    12181 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    12241 tctattgtgg tgaaaaataa cattcattgg gttggtcaac gtgactggga agtgcgtgat
    12301 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaatagcta cctcatccgc
    12361 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    12421 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    12481 gaggaccacg ctggggcgct gaccgaactg atggcacaaa ttcccgatac gccgatctac
    12541 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    12601 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    12661 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    12721 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    12781 gtggatcaga cggagctttt cgagcagtgc caacgttact acgccaatat cctgacgccg
    12841 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    12901 atgatagcca cttcccacgg cgtggtatgg cgcgataacc cgacgcaaat tgtcgagctg
    12961 tacctgaaat gggcggctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    13021 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    13081 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    13141 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    13201 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    13261 gctttcggat ctcacggctg gagcggcggt gcggtggatc gtctttccac gcgcctgcag
    13321 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    13381 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    13441 ccgcagagca cggtgaattc ggtggttaaa gaagaaacct ctgccaccac gacggctgac
    13501 ctcggcccac ggatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    13561 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag ttccggataa cttcctctgc
    13621 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    13681 acggcattgt gatcattggt tcgggcttcg ccgcccgaca actggtgaaa aatattcgca
    13741 aacaggacgc cactattcca ttaaccttga ttgccgccga cagcatggat gagtacaaca
    13801 aacctgacat cagccatgtt atcagtcagg ggcaacgtgc cgatgacctt acccgccaga
    13861 cggcgggtga atttgccgag cagtttaatc tgcacctgtt tccacaaacc tgggtgacgg
    13921 atatcgatgc cgaagcccgt gtggtgaaaa gccagaataa tcagtggcaa tacgacaagc
    13981 tagtactggc aaccggtgcc agtgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    14041 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    14101 gcgtgttgat tgttggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    14161 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtcg ttaatgccac
    14221 cggaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    14281 aatctcagtt acaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccgcc
    14341 agcgtagcat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaaccgccc
    14401 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    14461 ccagtaatac cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    14521 cgttcctcca gccgattcaa cttagcgcga tggtgctggc aaaaaatctt ctcggcaata
    14581 acacgccgct gaaactcccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    14641 atctggcagg cgaaacccag cgtcaggatt tacgctggca aattaatacc gaacgccagg
    14701 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    14761 atcggatgaa agaggcattt ggattgttga aaacattgcc gatgtaggtg ggctactgtg
    14821 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    14881 gccggataag gcgtttacgc cgcatccggc aatggtgtcc aaatgcaaca cgttttatcc
    14941 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    15001 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    15061 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    15121 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    15181 atgaaacgcc cacgcgcgtt gattaaccgg tgcctgccag ttcagccact gctgccagaa
    15241 agtggcgaga tccagttgat tgtccacccg cggcattgtc accggatgcg tcactccgtg
    15301 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    15361 tggcgcacag cccagtgccg ccgccactgc atcgaaaaaa cgcccacacg atgacgccag
    15421 cggcgcgtta attccacgct caatggcccg cgccagcacg ctccagtttt gctgttgcac
    15481 acttgctgtt tcgggataat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    15541 gtttcgccac ggctgcttcg ctgccaaatc gccacccgga agcgccactg caggcaagcc
    15601 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    15661 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    15721 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    15781 cggcagattc atttcacgcg cccactggct ggagacatag cccggatgcg cgtcatgcac
    15841 aacgtattgc ggggtaaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    15901 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    15961 gcgcaccagg cagaaggtat ttttcagatc cgcgccgaga cacagcacag gcggaacatt
    16021 tttaaagccc ggaggcaaag ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    16081 ttcgccgctt tcgcgcacca ccgaatcatc catccgctgc acgatgtcgc ggttatgtat
    16141 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    16201 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    16261 caaatgctgg agcgggttcg caggcaacat taccccgact tcgttaaggt caggggcgat
    16321 atcatcacaa agctcaggaa cgtatttttt atccaccagc acaatcggcg cggcgggcgt
    16381 ggtaagcaac tggcgcgcag cgtctggtaa accttctgcc actggcaaca taaccgccag
    16441 cggtttcgcc gggcgatgtt tgcgtgcccg cagtgtcgcc accgcgttac tgttacgtgc
    16501 atcgcaggca agatgaaatc cgccaatccc tttggtggcg acaatgttgc ccatttttaa
    16561 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    16621 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    16681 cgggtcacgg tactctttat cacaggccgg acacagcgga aacgccgcca tcacggtaaa
    16741 cgggcggtcg taaggcatgg cgcgaataat ggtgaagcgc gggccacagt gagtacagtt
    16801 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa gacaggcagg
    16861 acaagtagcg gcatcgggaa caatttgcgt attcatggtg ccgcctgtgc tctggcgtat
    16921 agtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    16981 cgccagcggc gggcagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    17041 ccggacttct acgccatcgc cgtcattaca gacatcgccg tgaagatcta attgctgtgc
    17101 caactgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    17161 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    17221 cgcccagccg gacacgacag cgtcgcatcc ggcagtcaca ggtcggcgat accgcccgct
    17281 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    17341 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattgcga
    17401 tcgacacaaa ttagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    17461 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagccccgcg
    17521 ccgctgttgc gaatcaccgg acgtaccacc acttccatcg cgccatacgg gcaagccaca
    17581 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg
    17641 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    17701 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    17761 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    17821 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    17881 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tactgttatt
    17941 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    18001 atgtgatgac aatgtttatc gcgaaggagc aatgagtgag tcgcggcgga tcagttttcc
    18061 gctgaaggtt ttcggcggtg tgtaagcacc cccatcgagc ataaaaatca gccgtccaat
    18121 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    18181 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    18241 gcgctcgtgc aacgctttca tcgcacctat cgccatatcg tcgttactgg caactaacgc
    18301 gctaaattta gccccacgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    18361 ccatttaccg ttagcgataa gttgttcatt gagcgcaata ccatgctgcg ccagcgcatc
    18421 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    18481 aatctcctga tgccgggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    18541 atcgcaccag acgctatggc tgctattttt gcgcaggcgg cgattaagca ccattatcgg
    18601 ctgactgtgc gcgtcaatga tgtcatcgat ctcatccacg cttaaaaaac gcgggtaaat
    18661 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    18721 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    18781 atgaaacagt aattcactaa aataaatgcc gtggtaaagc gtgttggtca ctaccagccc
    18841 cagcgtctga gtactcttcg ccgacagatt gcgcgccagc aagtttgggc ggtaaccgct
    18901 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    18961 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    19021 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    19081 gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg
    19141 aacaattcac tctcttgctt atttagtgac aactattcat gattttgtga aaccggtttc
    19201 ttaattccgt ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa ctacatctac
    19261 cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt
    19321 gatagcggca ctgggcggcg ttgataacat ctcggcggtc acgcactgta tgacgcggtt
    19381 gcgctttgtt atcaaagatg atgtgcttat cgacagcccg acgttaaaaa ccatccccgg
    19441 cgtgctcggc gtggtacgta gtgacaacca gtgtcaggtg attatcggca ataccgtttc
    19501 acaagccttt caggaagtcg tcagcctgct gccgggagat atgcagcccg cacagcccgt
    19561 gggtaaaccc aaactcacgc tacgtcgcat tggtgcgggg atcctcgatg cgctgatcgg
    19621 caccatgtca ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat
    19681 gatcctcgag atgagcggcg tgctgacaaa aggatcgccg accttaacca ttctgaatgt
    19741 gattggtgac ggtgctttct tcttcctgcc gctgatggtc gcggcatctg ccgccatcaa
    19801 atttaaaacc aatatgtcgc tggcgattgc catcgcgggt gtgctggtac atccgagttt
    19861 tattgaactg atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgattccggt
    19921 caccgcggtg aaatacacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc
    19981 atatatcgaa cgctgggtgg acagcattac accggcggtg accaaaaact tcctcaaacc
    20041 aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc cgattggtat
    20101 ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct
    20161 ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgcgt
    20221 ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggga tggtcatgcc
    20281 gtcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac
    20341 gaaaaacccg gaactgcgcc agacggcgct ggctgcggcg gcatcagcca ttatggcggg
    20401 gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc ttatcgccag
    20461 tcttatcagc ggttttattt gcggcgcggt tgccggtatg gcggggcttg ccagccactc
    20521 aatggcagcg ccggggctat ttaccagcgt gcagttcttc gatccggcga atccaatgag
    20581 catcgtctgg gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta tcctcacact
    20641 attgctcggc tttgaggata ttcctgttga ggaagcggct gccgaggcgc gaaagcatca
    20701 gagcgtacaa ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta
    20761 tttccagaag gttttttatg gggcggcgcg cttgccgcca accagtctga aggtgcgttc
    20821 cgtgaaggtg gcaaaggact gacaacggtc gatatgatcc cacatggcga gcatcgaatg
    20881 gcggtgaaac tggggctgga aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc
    20941 catgaggcga cggattttta tcatcgttat aaagaagata tcgccctgat ggcagagatg
    21001 ggattcaagg ttttccgtac ctcaattgcc tggagccgcc tcttcccgca gggcgatgaa
    21061 ctgacgccca accagcaggg cattgctttt taccgttctg tctttgaaga gtgcaaaaag
    21121 tacggcatcg aaccgctggt cacgttgtgc cacttcgatg tgccaatgca tctggtcacc
    21181 gaatatggct cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta cgcccggacc
    21241 tgctttgaag catttgatgg tctggtgaaa tactggctta ccttcaatga aatcaacatt
    21301 atgttgcata gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga aaatcaggat
    21361 caggtgaaat atcaggccgc gcatcaccag ctggttgcca gtgcgctagc caccaaaatc
    21421 gcccatgagg ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg taacttctac
    21481 ccttacagtt gcaagccgga agatgtctgg gcggcgctgg agaaagaccg ggaaaacctg
    21541 ttttttatcg atgtgcaggc gcggggcgcg tatccggttt actctgccca cgtattccgc
    21601 gaaaaagggg taaccatcga caaagcaccg ggcgatgatg aaatcctgaa aaacaccgtc
    21661 gattttgtct ctttcagcta ttacgcctcg cgatgcgcct cggcggagat gaacgccaac
    21721 aacagcagtg cggcgaacgt ggtgaaatcg ctgcgtaacc catatttaca ggtaagcgac
    21781 tggggctggg gaattgatcc actcggtctg cgtatcacca tgaacatgat gtacgaccgc
    21841 tatcagaagc cgctgtttct ggtggaaaac ggcctcggcg caaaagatga acttgctgcc
    21901 aatggcgaga ttaacgatga ctatcgcatc aactatttac gcgaacatat ccgcgcaatg
    21961 ggcgaagcga ttgcagatgg cattccgctg atgggctaca ccacctgggg ctgtattgat
    22021 ttagtttccg cctctacggg tgaaatgagc aaacgctacg gttttgtcta cgtagaccgt
    22081 gacgacgcag gcaacggcac gctgacgcgc acgcgtaaga aatcgttctg gtggtataaa
    22141 aaagtgattg ccagtaatgg tgaggattta g
//
LOCUS       DACAPB010000066        22014 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.066, whole genome shotgun sequence.
ACCESSION   DACAPB010000066 DACAPB010000000
VERSION     DACAPB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.066"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            18..377
                     /gene="yeaR"
                     /locus_tag="IT904_002225"
     CDS             18..377
                     /gene="yeaR"
                     /locus_tag="IT904_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HAP3191999.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            381..563
                     /locus_tag="IT904_002226"
     CDS             381..563
                     /locus_tag="IT904_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAP3192000.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            711..959
                     /locus_tag="IT904_002227"
     CDS             711..959
                     /locus_tag="IT904_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP3192001.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1018..1092)
                     /gene="yoaJ"
                     /locus_tag="IT904_002228"
     CDS             complement(1018..1092)
                     /gene="yoaJ"
                     /locus_tag="IT904_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAP3192002.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(1095..1193)
                     /gene="yoaK"
                     /locus_tag="IT904_002229"
     CDS             complement(1095..1193)
                     /gene="yoaK"
                     /locus_tag="IT904_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAP3192003.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(1226..2251)
                     /gene="dgcP"
                     /locus_tag="IT904_002230"
     CDS             complement(1226..2251)
                     /gene="dgcP"
                     /locus_tag="IT904_002230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAP3192004.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            2434..2688
                     /gene="yoaF"
                     /locus_tag="IT904_002231"
     CDS             2434..2688
                     /gene="yoaF"
                     /locus_tag="IT904_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAP3192005.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(2710..3057)
                     /locus_tag="IT904_002232"
     CDS             complement(2710..3057)
                     /locus_tag="IT904_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAP3192006.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3112..4292)
                     /gene="nimT"
                     /locus_tag="IT904_002233"
                     /pseudo
     CDS             complement(3112..4292)
                     /gene="nimT"
                     /locus_tag="IT904_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4389..5210
                     /locus_tag="IT904_002234"
     CDS             4389..5210
                     /locus_tag="IT904_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3192007.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(5167..5613)
                     /locus_tag="IT904_002235"
     CDS             complement(5167..5613)
                     /locus_tag="IT904_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP3192008.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            5783..5887
                     /locus_tag="IT904_002236"
     CDS             5783..5887
                     /locus_tag="IT904_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192009.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(5888..6391)
                     /gene="yeaK"
                     /locus_tag="IT904_002237"
     CDS             complement(5888..6391)
                     /gene="yeaK"
                     /locus_tag="IT904_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAP3192010.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(6434..7924)
                     /locus_tag="IT904_002238"
     CDS             complement(6434..7924)
                     /locus_tag="IT904_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP3192011.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQAGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(8105..9580)
                     /locus_tag="IT904_002239"
     CDS             complement(8105..9580)
                     /locus_tag="IT904_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP3192012.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFIYLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(9727..11010)
                     /locus_tag="IT904_002240"
     CDS             complement(9727..11010)
                     /locus_tag="IT904_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAP3192013.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYVLL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(11123..13057)
                     /gene="yeaG"
                     /locus_tag="IT904_002241"
     CDS             complement(11123..13057)
                     /gene="yeaG"
                     /locus_tag="IT904_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAP3192014.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            13493..14239
                     /locus_tag="IT904_002242"
     CDS             13493..14239
                     /locus_tag="IT904_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAP3192015.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            14329..15183
                     /gene="yeaE"
                     /locus_tag="IT904_002243"
     CDS             14329..15183
                     /gene="yeaE"
                     /locus_tag="IT904_002243"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAP3192016.1"
                     /translation="MQQKMIQFSGDVSLPAVGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(15231..16115)
                     /locus_tag="IT904_002244"
     CDS             complement(15231..16115)
                     /locus_tag="IT904_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAP3192017.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKENVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(16199..17194)
                     /gene="gapA"
                     /locus_tag="IT904_002245"
     CDS             complement(16199..17194)
                     /gene="gapA"
                     /locus_tag="IT904_002245"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP3192018.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            17536..17949
                     /gene="msrB"
                     /locus_tag="IT904_002246"
     CDS             17536..17949
                     /gene="msrB"
                     /locus_tag="IT904_002246"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP3192019.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            17991..18263
                     /locus_tag="IT904_002247"
     CDS             17991..18263
                     /locus_tag="IT904_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAP3192020.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGILAKPIAMFK"
     gene            18633..19709
                     /locus_tag="IT904_002248"
     CDS             18633..19709
                     /locus_tag="IT904_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP3192021.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            19736..21115
                     /locus_tag="IT904_002249"
     CDS             19736..21115
                     /locus_tag="IT904_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192022.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSEITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIVMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            21132..>22014
                     /locus_tag="IT904_002250"
     CDS             21132..>22014
                     /locus_tag="IT904_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAP3192023.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYA"
BASE COUNT         5790 a         5112 c         5427 g         5685 t
ORIGIN      
        1 ccgaaggagg gaaaaggatg cttcaaatcc cacagaatta tattcatacg cgctcaacgc
       61 ctttctggaa taaacaaact gcacctgccg gaatattcga acgtcatctt gataaaggaa
      121 cgcgcccggg ggtttaccca cgcctttccg ttatgcatgg ggcggtcaaa tatctcggct
      181 acgctgatga acacagtgca gagcctgatc aggtgatcct tatcgaagcg gggcagtttg
      241 cggtgttccc tccagaaaag tggcacaaca ttgaagccat gactgacgat acttatttca
      301 acattgactt cttcgtggct cctgaagtcc tgatggaagg tgcgcaacaa cggaaagtca
      361 ttcataacgg gaaatgaatc atgggcaaag caacgtatac cgtgaccgtc accaataaca
      421 gcaatggcgt ttctgtcgat tatgaaacag agacgccgat gactttgctg gtgccagaag
      481 tggcggctga agtgataaaa gatctggtga ataccgtacg ttcttatgac acggaaaacg
      541 aacatgatgt ttgtggttgg taattatcac tgatcgttga tggcgcaatt tctgcgccat
      601 cgcaaaaaaa acctccgtga tgctcgtcac aaatcttgcc ctcccgatgt tgtgctatgc
      661 tttttatcag cgactaaccc gctaatgcaa aacagataag tgaggaaaca atgggcattc
      721 tgtcatggat tatttttggg cttattgccg gtattctggc gaagtggatc atgccaggta
      781 aagatggagg tggattcttt atgactatcc tgctggggat agtcggtgcc gtagtcggcg
      841 gatggatcag cacgctgttt ggctttggta aagtcgatgg cttcaatttt ggcagcttcg
      901 tggttgccgt tattggtgcg attgtcgtgc tatttatcta caggaagatt aaaagttaac
      961 gcttaaattg cacaaaggct gcacacaggc agcctttgct attttttaga ggtgacgtta
     1021 ccaccatccc agctcagtgc cgagtacgac aataatcgcc acacccatca taataatcgt
     1081 tgtttttttc atctttatgc tcccggggca gcatagccgc caataaaaaa ccatgctaaa
     1141 aatacgaccg ctgtaatgaa gattacaacc ggaaaaataa taccgattcg catgttatca
     1201 ccaatcaaaa ggatgtgaat acgcctcagg aatgtagcgc tggatgcgcg acaaaaggtg
     1261 ttttctgttt gtgttttttc tcctgataca tcgcaatatc ggcagcatgc agggcactgt
     1321 ctgcatcggt tgtttcagga tcgacttcta ctatgccaag actggcaccg ggataataca
     1381 aatcaacatc acctaagtga tattctccac gtatttgctg ctgaatgcgt tctcgcagcg
     1441 acgaaatatc cgcattctcg ttgtttagtg aaacaactaa aaactcatca ccgccgagac
     1501 gaccaataac ttcgccattt tgctggagcg tattaaggcg ctcgccaacc tgaatgagaa
     1561 acagatcgcc actattatga ccaaaacgat cattgattaa tttgaagtta tccagatcga
     1621 taaacgcgat cattatctta tggttaagat gccgggcgag ggaaaacagt gtcgtcagat
     1681 tttcaaaaat cgcccgccga ttcggtagcc cggttaacga gtcggtatac gattgcgcaa
     1741 tcagcgcagc attggcttcg cgcagctgtt caaccagtgc ctctttttga atatattgtg
     1801 caatcagtcc ggcgaataac tgtaaaacct gttccgcgcg ttcactccac tggcgcttct
     1861 cactgctggc ggcgcaaagc gtgccataga atgatccatc cggtaagtga attggcgtgc
     1921 tcagaaatgt ggtgatgcca agattgcgtg ccgcaataca gtcaccccaa cggtcgggga
     1981 cttcatcgct gaaaaagcag ttttcatcaa tggcgcgttt gcataacgag taatcccacg
     2041 agacggtaaa attctccggg atgtgcattt tctggctgtt acgggcaaac ataatatgct
     2101 gcaggcgcgc ttcgacatcc actttggtca ggtaggttga ttccatatca gtgaccattt
     2161 ccagcatctc cagaagctgt cggaccagac tttccagtga ctgttctttg gcaagggatt
     2221 gcgagacgcg ggcgataatc tgatctgaca cggaacaact ccaggatacg gcgggtaaat
     2281 gctgattatt taatctttgc aggcatgata aaacatcatc aacaaagatg aaacaagacg
     2341 taatatgttt tgagtgaatg taataaaaat agtgctgcaa aacaaaacga taccgcgata
     2401 ctctaatctt ggataaatat gtgagatcaa taaatgaaaa ttatcagttt tgttctgcct
     2461 tgcttgctgg tcctggctgg ttgttcaacc ccttctcagc cagaagcacc taaaccgccg
     2521 cagattggta tggcaaatcc ggcgtcggtc tattgccagc agaagggcgg gacgctcatt
     2581 ccagtgcaga cagcgcaagg ggtcagcaac aattgcaaat taccgggcgg tgaaacgatt
     2641 gatgaatggg cattgtggcg gcgtgaccat ccggctggtg aaaaataacc gccggatgct
     2701 gtactaaaat cacaagctac gtagccagtc ggccagcacc agcgcatggt tctgcgtggt
     2761 gttttttgct gagtagagca gggtcagcgg ctgttttttg gcgatgtccg ccagccgctt
     2821 tccttcttgc tcgtgttgcg ccagttctgc aagatattgc tcgcgaaagg ttgcaaaatc
     2881 gacgacttcg ccgtgaaagg ctttgcgcag ttccgttgac ggcgtgattt ctttatccca
     2941 ctcatcaagg gctaaatcgg tttttttgat accgcgcggc cagaggcggt cgaccagtat
     3001 gcgataacca tcgctctgtt cagccggatc ataaacgcgt ttgcactgaa tattcatcgt
     3061 gtttgttctc cctttcacgc atgatttgtt acgtcactat agcaccggat attagcgaat
     3121 ttctttgtct ctcccggcgc aaagtccgaa aatcgccatc agtagtgaaa gtatggcaac
     3181 acccataagt ggtacagacc agttaccgtt agcatcgtgt attttaccca tcagcggcgg
     3241 cccacaggct gccaacaaat acccgacgga ttgtgccatc cccgagagtg ccgccgcctg
     3301 atgcgcagaa cttgcccgaa gaccaatgaa cgtcaacccc agtatcattg ttgcgccgga
     3361 accaaaaccg aaaagcagag tccaggtgat cgcgtgcgct ggcataaagc agagcccaac
     3421 agcgcccact gcgcacatca aggcaacgaa cgctgcaata ccacgctgat ctttcacatg
     3481 atgtaagaaa agagggatca gcaaaccggg agctgctgtg gctagttgca gcaaaccatg
     3541 cagtgaaccc gcctgtgctt cgctatagcc gtgactgatg aggatcgccg gaagccagcc
     3601 aataatcacg taatagacca gtgagttgat cccaagaaac aatgtgacct gccaggcaag
     3661 cggcgaacgc cagatacccc gagtatgtaa ggcgcgcgag gtactcaaat ttgcatgttg
     3721 ttggcttcgc cactgtggca gccataaaaa agagccagca gaggaaaaca catcagcatg
     3781 agcaacgcgc cttgccagcc aaaaccgttc aaagccagcg gcacgaccat agccgatcca
     3841 agtgccgccg cagctcccat tgtcagggaa tatgcgccgg taagtctggc gacggaatga
     3901 gggaaatcgc gtttaattaa tcctggcagt aagacattgc ctaatgcaat cccaccgcca
     3961 atgaccgctg taccgccaaa taataagtaa ggcgaaggga gagagcgaat tgcgatacca
     4021 gcacagatca aaagtaacgc ggcaaacagg ctacgttcca taccaaaacg tcgcgctacc
     4081 ggggcggcca aaggtgagat tagcgcaaag gccaataatg gcagggtggt caataagccg
     4141 gtttgcgctg tcgtcagcga gtaagcggaa cgaatcgtat cgagtaacgg tgctgcgccg
     4201 gtgaaggtga cgcgtaatgt tgtggcaatc atcagaatgc cagcgataag gacaatcctg
     4261 tttttgccgc ttaatgaagt tgaacaggtc atatgcttct cattgttaca aacgaggctg
     4321 taccttaccg tttttattgt tatgttgaat caagctaaaa tgacaaatta tcgctaaatt
     4381 cggacaaaat gatgcacagg ttgaatctca atggctatga acccgaccga caccatgaag
     4441 ccgcggtcgc attttgtatt cacgccggaa cggatgagtt aaccagtcct gtacatcagc
     4501 accgcaaagg acagctgata ctggcgctac atggtgcaat tacctgtacg gtggaaaatg
     4561 ctttgtggat ggtgccgcca caatatgcgg tttggatacc gggcggggtg aagcatagca
     4621 atcaagtgac agctaacgct gaactctgtt ttctgtttat cgaacccagc gcagtgacaa
     4681 tgccgacaac atgttgcaca ttgaaaatat cgcccttatg ccgggaattg attttaaccc
     4741 tggcaaacag gacgacgact cagcgtgctg agccgatgac ccgcagatta attcaggtcc
     4801 tgtttgatga actccctcag cagccacaac aacagctgca cctgcccgtt tcttctcatc
     4861 ctaaaatccg cacgatggtg gagatgatgg cgaaagggcc cgtcgagtgg gggtcattgg
     4921 ggcaatgggc tggctttttt gcgatgagtg aacgcaatct tgcccgactg atagtaaaag
     4981 agacggggtt gagttttcgc cagtggcgtc agcagttaca gctgattatg gcgcttcagg
     5041 gattagtgaa aggcgatact gtacagaaag ttgctcatac gctggggtat gattcaacaa
     5101 cggcctttat tactatgttt aagaaaggcc tggggcaaac gccaggccga tatattgcgg
     5161 gattaactac tgtttcccca caatcagcga aaccagaccc gcggcaataa gcgggccaac
     5221 cggtacgccg cgaaacagcg ccacgcctaa aacagtgcct accagcagtc cggcgaccag
     5281 ttgcggctgg ctgcccatta acgtcacgcc gcgcccaccc agccaggaga caataacgcc
     5341 tactgcaatt gccaccagcg atttccagtt cagaaacgag tgaatcaatg ttgagggcgg
     5401 tagcgtcccg ctggcgatgg gggccataac gccaatcgtc agaatgatta tcccgatact
     5461 tagcccctgt ttttcaatcc acgggaaaaa ggtgcttaac ggtgtgacgc ggacaatgat
     5521 caacaccaga attgaaacgg cgacagtggt gttatgactg ataaagccca gcgcagctaa
     5581 cccgagcagg atcagcaaag tgacatcaaa catcgtggtt tccttgccaa aaaataataa
     5641 gcctgcttac tttacgcttg ctgcaggaag gaagtagaaa atctttacgt tttttgcata
     5701 aaaaatcagg cgaactgtca tcaaactgcc atttgtgcac gctaacaata acgtgaaaac
     5761 agctatctcc cgggggcatg ctatgaacga tcaaatgttc gtcgagacac tgattatcac
     5821 gtcatcgttt tttgccattg ctgttgtgct ggttttgtcc gttctgctta ttgaacggac
     5881 aggctagtta cgcagtgcgg cggaaattga ccagttctgg ttgcgcaatg cgcaaataat
     5941 ccgcggtttt caaaataacg gatttatcca gcatacctgc attgaaggcg atttcatcaa
     6001 atcgttcgaa gagtagaggg tcggcaacca gtttgagttt tggatggaag ctgaaagggg
     6061 ggatcgcgcc gaagacacag cctgtgagtt catcgacttc cgccgggctg gccaacgaag
     6121 cgcgtaatcc gcctatatgg ctggcgagtt ggctcagatc ggcctgttga tcggctgcga
     6181 ggatcgccag aacatgttga tttacgccat tgcctttgac tttacatacc agtgccttag
     6241 ctccctggcc taaagcggtg ccacgaattt ctgacaccgc ttcacattta cctaccgctt
     6301 catgggtaac gacgcggaag ttagcacctt cttgtgataa taaagcgatt aaccgctgat
     6361 gcgtcacgct tcctttagcc atttcagtca tttcacgctc ctgagattac aagcaaacaa
     6421 ccacagaagg ttatcatgaa cggctgtttt tgttctgctt attgacatat aaacgctcat
     6481 cggaagcttt atacgcatca tgtaacgtat cgttttcttt catcgcgtaa ataccggaag
     6541 agaagccaat ctctttctgc ggcgcgatat gttgcagacg tttttcgata cgttcaggca
     6601 gttgtgctgc aatttgcggc gtcgaatcga caagaatgat gcagaattca tcgccaccga
     6661 gtcggatggc ataatcactt ttacgaatcg attgtttaat cgcctgagct aataacgtaa
     6721 tcgctaaatc cccctcctga tgaccgaggg tgtcatttat ttgctttaac ttgtccatgt
     6781 caattgcaat aaacatcacc gaagaaccgg cttgcaccag tttctgcaac cgctgctcca
     6841 gttcaggggt taaaattttg cgattataca gtccagtcat cgcatcacta atattttctc
     6901 gcgacacatt ttggtataaa cggaagtgca tccgcaccat attcagcaaa attaacgccg
     6961 tcaaaatcca gaacagaacg cttttccacg aagacgtgat aaagtaaaga atatcaatgg
     7021 ataacgagac acgaatgccg cccggtaagt catggacgta actgacatac tggaacagat
     7081 tatcttcgct ctggttgatg ataatgtcgc gccccgaatc ggtatcggtt agcgtgacat
     7141 tgagaaaacg ccagaggaga gggcggtcat gagtgtaaaa gatattccgc aaattgtttt
     7201 tgttaatatc cagcagcaca atccctttta gctgccctgc gacatatacc ggcgtcagca
     7261 aactcatcac gttctgttta gtacgctgat cctggtaaat gctcgacaat acggtacgcc
     7321 cggtaaatac cttttcaata tctttgcgat cgatgccaat acttcctgac tgcaggaagg
     7381 accagttatt catcgcaaag tattcaacat taaccggctc ataaaaatag acatagcggt
     7441 cattgagatc gagataatag tgaaatttct cttcggccat cccaagacct gaaccgtaat
     7501 catcctggtg gcggttgcct tccactgcct gatcaaaagc gggtaatagt gctgcagctt
     7561 ccacaattgt gtcgcagtca gtggatgcgg tttgtagtgt gccgcgtaag cccgggtatt
     7621 tatgggcagt caggtttaag ccatgggtat tattggcaga ctcaaaagcc tcgcagatgg
     7681 cgcgccgctg ttctggcgac acttccgact gctcagcagc gagagcgcgc atcacatgcg
     7741 cggcaatact ctgattctga tacttatcat aaagaaagga ggagtctgct ttctgcacga
     7801 tatagcgcag ataggcactc attgctctgt cgctggcgac aagttcaaaa ataaggaagg
     7861 aagatgtcag cacaatgaca cttgcggcga taaaatgccg gagcattcta tggtgcaatt
     7921 tcatgataaa tggcactcct tcggttagtg aaacgagaaa accagataaa gttcgattgc
     7981 ggcctggcac aggggcgcta attatactca tctctgcgcc gctggggagg ggaaatttag
     8041 gggtaatagt aataaattgt atgcaaaagc catgcaaaaa gcatggctct gaatattttt
     8101 gcacttaaat atgatgaata atcactttgt tgcccccctc gcttttcgct tcgcgtaagg
     8161 ctttatctgc ttccgtcatg acaagcgaga tattacgcgt gtcacccgtt gaaaaaaccg
     8221 tgccaatact gatggtcatt tgttcaggaa catcatatct gttactaaag ccggtcagga
     8281 gttcgacatt tttacgcata cgttccgcaa tgatttttgc ctgggcacta ttgagttcgg
     8341 taaatagcaa gccaaacacc tcaccctcca gtcgcgctaa aatatcatcg gggcggatgc
     8401 tttgctggat aatatcgaca attgaaacca gcactttatc acccacacga tgtccccatt
     8461 gggcattgat gcgtttgaac tgattaatat caaccagcat gacacagaag tccttaacaa
     8521 caggtcgcga caataatgac tctacgctgt tgaaaaagta gcgccgatta taaatattgg
     8581 tcagcacatc atgaactgcc agtttgctgg agagttgcag ttcctgaaaa atgttataaa
     8641 tcagaaaaga cacaacaaac agtttactgg agacttcaat cccgcgactg atgtaccaga
     8701 tggtgtaatt atactcatcc agagtcagta gaagtaagtt gcagcatagc gatgccagac
     8761 ataataatgc tattaatggc catagttcgc tggctaatcg tgtacgcatg ataataaaga
     8821 acagtaaaaa tgcccacaga caaaccaata tttttgtata gttgattccc caggtcgcag
     8881 tatggttgtc aggacagtag tcgacgacat acaaagaata gtcagcgtta taactgctca
     8941 gattgtgtgc cagaagggga aaaactaaaa aaggaatcag cgccagcaac aggattcccg
     9001 tttttttctt attgttgtca aggatgttgt cttttccata acaaaagagt gccagcgagg
     9061 ttaaataaat aaaactgagt tggcggaaaa tatagaaaat agaaacatca ttcgactttg
     9121 tctggatcag tgtactgccg ttaattggtt tatggataat tacaatggtt tcaactaaat
     9181 agataaggcc acttaaaaat gcaaggctta aaatgagtgt tggtaaaatt tgtttgttag
     9241 tgaaatattt cattaacatg aaaagaaaaa tgacggcgtt aaggaaaaac aaagccacaa
     9301 caaccatcag gtaattcatc tgtgggatga gttttacatt tgtgttttcg ttatataagg
     9361 tagtaatgca aaataaaatt aacgtcagca ataaactgaa aataaaaaag ctttgagctt
     9421 ttaatttttg tgtacccata gaaaccataa aaaggagagc aatgtgagtg ctggatttta
     9481 ttgatggcag agatatatag ttttgaccaa gatcaatttt cgttaacgat aaaaaatatt
     9541 taaagaataa ccccatggaa atacgtgttg actgaatcat ttttccgaat gattgttttc
     9601 tggaactaaa ttatgtaaag gttatgctgt catatttaaa gcgattgtaa gctaatgtat
     9661 gtaataaatg agataattta cagtaagcta aaaatcagcc aggcgataat gacctggctg
     9721 atagttttag tctttagctg ttgcattttg tttatgaaac agttcacgga acaccggata
     9781 aatatcatcc tggtcgcgga tgtgctgcat cgcaaagttg tcgaaagtag attgcagatg
     9841 ctcatattct cgccacaatg tctgatgtgc acgacgggta atttcgatat agctgtaata
     9901 acgaacaaca ggtaataatt ttttcgccag gatttcatgg caaagcggag agtcatcggc
     9961 ccagttatcg ccgtccgatg cttgtgcagc gtaaatattc cactgtgccg ggttataacg
    10021 ctctttcact acctcatcca tcagtttcag ggcgctggaa acaatggtgc cgcctgtttc
    10081 ctgcgagtag aaaaactcat gttcatcgac ttctttcgcc tgggtatgat ggcggatgta
    10141 tacgacttcc acgttcttat acgttctgct gaggaacaga tacagcagaa cataaaaacg
    10201 cttagccata tctttagtgg attgatccat tgaaccggaa acgtccatca ggcaaaacat
    10261 cactgcctgg ctggagggat cgggccgctt ctcgtagttc ttgtaacgta aatcgaaggt
    10321 gtcaataaaa gggacgcgtt caattttggc acgtaattct gcaatttctt tgcgcagacg
    10381 ttcctcttcc agcagttgcg caggttcact gttgctgatg atggccaaat tctcttccag
    10441 tgcatgaagt tcccgccgct tgcctgccgt catggctgtg cgtcgcgcca gtgagttctg
    10501 caatgaacgc acaacgctga tattggccgg aacgccgtta gcggtataac ccgcccgatg
    10561 cgttttatat tcggtcagct ggcgttgttg gttttgtttc agattcggta aggccaaatc
    10621 ttcaaagagc agatcaagat actcatcttt cgaaatctga aagacaaatt catcctgacc
    10681 ttcaccatcc tggctggcct ggccctgacc actgccggaa cctccgccgc caccctgggg
    10741 acgttcaatt cggtcgttct ggacgaaatg gtcattgccc ggatgcacgc ggtggcgcag
    10801 accgccacgc ccctgatgaa acatcggttc gctaatatct tccgtgggaa tggatacgga
    10861 ttcgccgctg tcgacgtcag tcaccgaacg cttattaatg gcctcggaga tcgactgttt
    10921 aatttgcgct ttataacggc gtaaaaaacg ctggcgattc accatgcttt tgtttttgcc
    10981 gttcagacgc cggtcaataa accaggtcat atgccccccg tactacattt gccaactgta
    11041 tgctacgcct gtgttgccgc ttctcaggcc cacggttcgc tgtcgttgta ggcccagtaa
    11101 catgtgtacc gggccagggt tgttaagacg atttacgtac gcgcaaatac cattcgcaca
    11161 gtaaacgcac ctgtttacgg gtgtagcctt tttccatcat acggtcgaca aagtcgtcgt
    11221 gtttcttctg ctcgtcggtt gacgttttgg cgttaaacga gataaccggc aacagctcct
    11281 cggtattgga gaacattttc ttctcgatga ccgtgcgcag tttttcatag ctggtccagt
    11341 tcggattgcg tccgctgtta ttcgctctgg cgcgcagtac gaagttaaca atctcgttgc
    11401 ggaaatcttt tggattactg atccccgccg gtttctcgat tttctccagc tcggcgttaa
    11461 gagactcgcg gtcaaacagc tgcccggtat ccggatcgcg atactcctga tcctgaatcc
    11521 agaaatccgc gtaggtaaca taacggtcga aaatgttttg cccatattcg gaataggatt
    11581 caaggtaggc cgtctggatc tctttgccga taaactcggc atatttcggg atcagataac
    11641 ctttcaggaa ctccagatag cgttctgcct gctcttgtgg gaactgctcg cgctcaatct
    11701 gctgttccag gacgtagaac agatggaccg ggtttgctgc cacttctaca tgatcgaagt
    11761 tgaacacgcg ggagaggatc ttaaacgcaa aacgcgtcga cagaccgttc atcccttcat
    11821 cgacaccggc gtagtcacga tattcctgat acgacttggc tttgggatcg gtgtctttca
    11881 gactttcgcc atcataaacc cgcatctttg aataaatgct ggagttttct ggctctttca
    11941 ggcgcgaaag aatggaaaaa cgtgacagtg tttcgagcgt gccaggggcg catggggcgt
    12001 gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg gaaatgcgca
    12061 agcaatacgg caccttcacg atgtaaacac gatcgaggaa ggcttcgttg tttttgttat
    12121 tacggaaagt gacccattcg gactcgttcg agtgtgcgag aataatcccg ttgaacggca
    12181 gggcggagat accttccgtc ccgttgtagt taccttcctg agtggcggtt aacaagggat
    12241 gcagcacttt aatcggtgct ttaaacatct caacgaattc catgatcccc tgattggcgc
    12301 ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag tgttcgagtt
    12361 tacgaatatc gactttccca accagcgcgg agatgtcctg gttgttctca tcgccgggtt
    12421 ccgttttggc gatagcaatt tgttgcagaa ttgacggcca gaccttcact acccggaact
    12481 tagtgatatc gccaccaaat tcatgcaggc gttttgccgc ccacggcgac atgatggtgc
    12541 cgagataacg gcgaggaatg ccatactctt tttccagaat ctgcgcatct tcctgcggat
    12601 tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttcgcg ctcaatacat
    12661 aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat ttacccccac
    12721 ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga gccgcgtgtt
    12781 tcagataaga gacaatctgt tcaatcgcgt cttccatgcc gtaaaactct tcaaacgccg
    12841 gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctgggttcc tgggctgtat
    12901 cgaccatgac aggctcaccg atagccatca atagccgctc agccgcgttg gcataagcac
    12961 tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg tccttggcag
    13021 cttcatatcg ctggcgatag tgatcgaata tattcatggt atgccgtcct ttcgtttttt
    13081 agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg taaactaaac
    13141 gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt
    13201 gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg
    13261 ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa
    13321 agaccaggct cgccagtaac gctaaattca tttggctgta agcgcggtgt catccgcgtc
    13381 aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat
    13441 tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa
    13501 actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga
    13561 aggtaaattt tccctgggcg caggcgtagg tgtcgttgaa cacccatata aagattacga
    13621 taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg
    13681 cttaggtggt ggttactacc tgtggaatga cgcaacggat aaactttcaa ttaccgctta
    13741 ctggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga
    13801 tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta
    13861 cctgcgtacc accctggctg gcgataccct ggataacagc aacggcatcg tctgggatat
    13921 ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta ttggtgtgca
    13981 gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg
    14041 cagcggtctg cgtggctata acccgaacga cagctggagc ccttacctgg agctgagcgc
    14101 cagctacaac ttcctcggcg actggagtgt ttacggtacc gcacgctaca cccgtctgtc
    14161 tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg
    14221 gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc
    14281 gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa
    14341 atgattcaat ttagtggcga tgtctcactg ccagccgtag ggcagggaac atggtatatg
    14401 ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcggg cattgaactc
    14461 ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt
    14521 ggggaagcat taaccggtct gcgagagaag gtctttctcg tctctaaagt ctatccgtgg
    14581 aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact
    14641 gattatctcg atctttactt attacactgg tctggcagtt tcgcttttga agagactgtc
    14701 gcagcgatgg aaaaattgat cgcccaggga aaaatccgcc gctggggcgt ttctaacctt
    14761 gattatgctg atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat
    14821 caggtgcttt accatctcgg ttcacgagga attgagtacg atctactccc ctggtgccag
    14881 caacagcaga tgccggtgat ggcttacagt ccgttagccc aggccgggcg gttgcgcaat
    14941 ggactgttaa aaaacgcggt agtcaacgaa attgcacatg ctcacaatat cagcgcggca
    15001 caagtattgt tggcgtgggt gatcagtcat cagggtgtga tggcgattcc aaaagcggcc
    15061 acgattgccc atgtccaaca aaatgcggct gtgcttgagg tcgaactttc ttcagcggaa
    15121 ttagctatgc tggataaggc atatccggca ccaaaaggaa aaactgcgct ggatatggtg
    15181 tgatgttgtg tgccggatgc atcgcacccg gcacacaaca ttaacgtaaa ttaacgtttc
    15241 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtcacttt ttgcgtttct
    15301 gaagcgtaag ccgtttctac acaaacaaat gttttgtagc catcatccgg catatcgccc
    15361 atgctaattg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
    15421 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgtggattc
    15481 agatacacgc gatcggtacg gtcagggaag gtctgaatac catcggtcag tacattttct
    15541 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
    15601 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
    15661 tcaagatcga tttcacaagt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
    15721 ggccagaatt ttttcgtctc ttcgctttgc gtcaattcaa aagtcagcgc tacgccatca
    15781 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
    15841 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgcc accgcgaata
    15901 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
    15961 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
    16021 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
    16081 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
    16141 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
    16201 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
    16261 accaggatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
    16321 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
    16381 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
    16441 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
    16501 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
    16561 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
    16621 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
    16681 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg
    16741 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
    16801 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
    16861 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
    16921 cgtcaacacc aacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
    16981 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
    17041 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
    17101 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
    17161 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
    17221 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
    17281 tcattgcgac aaatcaatcc tgtgcctaag cattacgcga ctgactcgcc tcactcttcc
    17341 tttgggcttg agaccacatg gggtcggtgc cccgaatttt aaagggcaat tacgataaaa
    17401 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
    17461 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
    17521 gtgagatgtg agcaaatggc taataaacct tcggcagaag aactgaaaaa aaatttgtcc
    17581 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
    17641 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
    17701 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
    17761 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
    17821 ggtaactgtg atgctcatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
    17881 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
    17941 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
    18001 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
    18061 tggggaaatg gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac
    18121 tggtgatgct gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa
    18181 atggtcagat ggtcatgaag agcaaacagc agttaaaaga agattttggt atcttagcaa
    18241 agcctattgc tatgtttaag taagtggttt atcccctttt tgccgcggct atgaagggga
    18301 tagccaacaa tgaccccacg actcgtctca attatctcgc cccgacctgg ctaagtccca
    18361 gtccgatctc ttgccgtcgc gttcacgcct gaatgacttc tcctgaactt aagtctcaga
    18421 cctatttggc cggtaatccc tctcgaattt gctctttttg cgtcccgcct tccatcaacc
    18481 tgtaacagac cgagccattt tctgcgtaat tgtgatctaa gttgtaaaac gcagatcatt
    18541 atctgtgttt tacagattta atgttgacct caaaaatcat gtggataagt atgatggcca
    18601 tctaagcaaa acaaaacaca gaggatgacg caatgaaagc actggctcgg tttggcaagg
    18661 cctttggcgg ctacaagatg attgatgtcc cacaacccat ttgtggcccg gaagacgtag
    18721 tgattgaaat taaagccgcg gcaatctgcg gcgcagacat gaagcactac aatgtcgata
    18781 gcggttctga tgagtttaac tctatccgcg gccatgagtt cgcaggttgt attgcgcagg
    18841 ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc
    18901 acgtttgcgg cgtttgtccg gcctgtgaac agggtgattt tctgtgttgt acagaaaagg
    18961 taaaccttgg tctggataac aatacctggg gcggtggttt ttccaaatat tgtctggttc
    19021 ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga aatccctgat ggtgttgatt
    19081 atgaggacgc agccgtactt gaccctatct gtaatgccta taaatccatc gcgcagcaat
    19141 cgaaattcct tcctggtcag gatgttgtcg tcatcggcac tggcccactc gggctgtttt
    19201 ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag
    19261 atgtggcggt ccgcttcccg gttgcaaaag aactgggggc gacggcagta gtaaatggtt
    19321 ctaccgaaga tgtggtggcg cgctgccagc aaatttgtgg caaagataat ctgggactgg
    19381 tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc catcgaaatg ctccgcccga
    19441 atggggaagt ggtacgcgtt ggaatgggct tcaaacctct tgatttctcg attaatgaca
    19501 ttaccgcctg gaataaaagc atcattgggc atatggccta tgactccacc tcatggcgta
    19561 acgctatcag gctattagcc agcggcgcta tcaaagtcaa accgatgatc acgcatcgta
    19621 tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt cgataaaacc gcaatcaaag
    19681 tgatcatgac ttacgacttt gatgaataac cactctcctg aaggaacgtt atcgcatgga
    19741 acagataaca aaaccgcatt gtggtgcacg gctggatcgc ttacctgatt gccgctggca
    19801 ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg
    19861 tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca gataattcca ccacagccac
    19921 attctcagaa atcacgaccg ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg
    19981 cgacaaaacc ggtcgtagaa atgcgttcat cctctatgag gccattcata ttgcctcgat
    20041 ggttgtcggt gctttctcac cgaatatgga ttttcttatt gcctgccgtt ttgtgatggg
    20101 cgttgggctg ggagctttac tggttacgct gtttgctggt ttcaccgaat atatgcccgg
    20161 tagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt ggcaactggt catatccgct
    20221 ctgttcattg attgtgatgg gactcacgcc gctgattagt gctgagtgga actggcgggt
    20281 acaactgctt atccctgcaa tattgtcgct tatcgctacg gcgctggcct ggcgctactt
    20341 tcctgaatcc ccgcgctggc tggaatcgcg cggacggtat caggaagccg agaaagtcat
    20401 gcggagtata gaagaaggcg tcatacgcca gacgggtaaa cctttgccgc ctgtggttat
    20461 tgctgatgac ggtaaagcgc cacaagcggt gccgtattca gccttactga caggagtatt
    20521 actgaaacgc gtgatattag gctcttgtgt gctgattgcc atgaacgttg tgcagtacac
    20581 actaattaac tggttgccaa caatattcat gacccagggg attaatttaa aagactcgat
    20641 tgttttaaat accatgagta tgtttggcgc gccatttggt atttttattg ccatgctggt
    20701 gatggataaa attccgcgta aaacaatggg tgtggggcta ttaatcctga ttgcggtgct
    20761 cggatatatc tattcactgc aaaccagtat gttgctcata acgttgattg gtttcttcct
    20821 gattactttc gtctatatgt acgtttgcta tgcctcggca gtgtatgtcc ctgaaatctg
    20881 gccgacagag gccaaactcc gtggctccgg tctggcgaat gcggtagggc gaatcagtgg
    20941 tattgccgca ccttatgccg ttgcagtgct gctcagtagt tatggcgtaa cgggagtctt
    21001 tattcttctg ggggctgttt caattgttgt cgcaattgct atcgccacca ttggaattga
    21061 aaccaaaggt gtctccgttg aaagtttaag tattgatgca gtagccaata aatgatacgg
    21121 gtggattcaa aatgaaaaat tcaaaagcaa tattgcaggt gccgggcaca atgaaaatta
    21181 tttcagcaga aataccagtg cctaaagaag atgaagtttt gattaaagta gaatatgtcg
    21241 gtatttgtgg ttcagatgta catggttttg aatcaggccc gtttattccg cctaaagacc
    21301 caaatcaaga aattggcctg ggtcatgaat gcgccgggac ggttgtggct gtgggaagcc
    21361 gcgtgcgcaa atttaaaccg ggggatcggg taaatatcga acctggcgtt ccttgcggtc
    21421 actgtcgtta ctgtctggaa ggcaaatata acatctgccc ggacgttgat tttatggcga
    21481 cacaacccaa ctaccgcggc gcattaacgc actatctgtg tcatccggag agctttactt
    21541 acaaactgcc agacaatatg gacacgatgg aaggggcgct ggtggagcct gccgcagtcg
    21601 ggatgcatgc cgcgatgctg gcagatgtta aaccgggtaa gaagataatt attctgggag
    21661 caggttgtat tggtttgatg acgttgcaag cgtgcaaatg cctgggagca acggaaattg
    21721 ccgtcgttga tgtgctggaa aaacgtctga taatggcgga acaacttggt gcgacagtgg
    21781 ttattaacgg cgcaaaagaa gacactattg cacgctgtca gcaatttacc gaagacatgg
    21841 gcgcagatat tgttttcgaa acagcgggtt ctgcggtcac cgttaaacag gcaccttatc
    21901 tggtaatgcg cggcggtaaa attatgattg ttggtactgt accgggcgat tcggcaatca
    21961 atttcctcaa aatcaatcgc gaagtcacta tccagacggt attccgctat gcca
//
LOCUS       DACAPB010000067        21144 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.067, whole genome shotgun sequence.
ACCESSION   DACAPB010000067 DACAPB010000000
VERSION     DACAPB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.067"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            288..1331
                     /gene="nadA"
                     /locus_tag="IT904_002251"
     CDS             288..1331
                     /gene="nadA"
                     /locus_tag="IT904_002251"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAP3192024.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVEERLRERALVP
                     LNRMLDFAATLRG"
     gene            1369..2088
                     /locus_tag="IT904_002252"
     CDS             1369..2088
                     /locus_tag="IT904_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAP3192025.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2085..3026)
                     /gene="zitB"
                     /locus_tag="IT904_002253"
     CDS             complement(2085..3026)
                     /gene="zitB"
                     /locus_tag="IT904_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAP3192026.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3140..3520)
                     /locus_tag="IT904_002254"
     CDS             complement(3140..3520)
                     /locus_tag="IT904_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192027.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3836..4888
                     /gene="aroG"
                     /locus_tag="IT904_002255"
     CDS             3836..4888
                     /gene="aroG"
                     /locus_tag="IT904_002255"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAP3192028.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(5046..5798)
                     /gene="gpmA"
                     /locus_tag="IT904_002256"
     CDS             complement(5046..5798)
                     /gene="gpmA"
                     /locus_tag="IT904_002256"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP3192029.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6001..7041)
                     /gene="galM"
                     /locus_tag="IT904_002257"
     CDS             complement(6001..7041)
                     /gene="galM"
                     /locus_tag="IT904_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAP3192030.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(7035..8183)
                     /gene="galK"
                     /locus_tag="IT904_002258"
     CDS             complement(7035..8183)
                     /gene="galK"
                     /locus_tag="IT904_002258"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP3192031.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8187..9233)
                     /gene="galT"
                     /locus_tag="IT904_002259"
     CDS             complement(8187..9233)
                     /gene="galT"
                     /locus_tag="IT904_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAP3192032.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9243..10259)
                     /gene="galE"
                     /locus_tag="IT904_002260"
     CDS             complement(9243..10259)
                     /gene="galE"
                     /locus_tag="IT904_002260"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP3192033.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10521..11993)
                     /gene="modF"
                     /locus_tag="IT904_002261"
     CDS             complement(10521..11993)
                     /gene="modF"
                     /locus_tag="IT904_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAP3192034.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(12061..12849)
                     /gene="modE"
                     /locus_tag="IT904_002262"
     CDS             complement(12061..12849)
                     /gene="modE"
                     /locus_tag="IT904_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAP3192035.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSCARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12978..13127
                     /locus_tag="IT904_002263"
     CDS             12978..13127
                     /locus_tag="IT904_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAP3192036.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13294..14067
                     /gene="modA"
                     /locus_tag="IT904_002264"
     CDS             13294..14067
                     /gene="modA"
                     /locus_tag="IT904_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3192037.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQFKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASVQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTIK"
     gene            14067..14756
                     /gene="modB"
                     /locus_tag="IT904_002265"
     CDS             14067..14756
                     /gene="modB"
                     /locus_tag="IT904_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAP3192038.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14759..15817
                     /gene="modC"
                     /locus_tag="IT904_002266"
     CDS             14759..15817
                     /gene="modC"
                     /locus_tag="IT904_002266"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAP3192039.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15818..16636)
                     /locus_tag="IT904_002267"
     CDS             complement(15818..16636)
                     /locus_tag="IT904_002267"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAP3192040.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVIKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16791..17786
                     /gene="pgl"
                     /locus_tag="IT904_002268"
     CDS             16791..17786
                     /gene="pgl"
                     /locus_tag="IT904_002268"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAP3192041.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17827..18780)
                     /locus_tag="IT904_002269"
     CDS             complement(17827..18780)
                     /locus_tag="IT904_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3192042.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEAPELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLMEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18964..20016
                     /locus_tag="IT904_002270"
     CDS             18964..20016
                     /locus_tag="IT904_002270"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAP3192043.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20092..>21144
                     /locus_tag="IT904_002271"
     CDS             20092..>21144
                     /locus_tag="IT904_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAP3192044.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAE"
BASE COUNT         5042 a         5219 c         5527 g         5356 t
ORIGIN      
        1 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctattcag gcatctctca
       61 atttcaattc ttgaccctga tgacatactc cgcgcttgct gcgtaaacaa ctttacgtcg
      121 gaggaataag acgcaaagtg tggcattcgg catctcatac gtaattcgag tgacttttcc
      181 ccaccattcg actatcttgt ttagcatata aaacaaatta caccgataac agcgaatatt
      241 acgctaatgt cggttttaac gttaagcctg taaaacgaga tggtaagatg agcgtaatgt
      301 ttgatccaga cacggcgatt tatcctttcc ccccgaagcc gacgccgtta agcattgatg
      361 aaaaagcgta ttaccgcgag aagataaaac gtctgctaaa agaacgtaat gcggtgatgg
      421 ttgcccacta ctataccgat cccgaaattc aacaactggc agaagaaacc ggtggctgta
      481 tttctgattc tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct actttgttag
      541 tcgctggggt gagatttatg ggagaaaccg ccaaaattct cagtccggaa aaaacaattc
      601 tgatgccgac acttcaggct gaatgttcac tggatctcgg ctgccctgtt gaagaattta
      661 acgcattttg cgatgcccat cccgatcgta ctgtcgtcgt ctacgccaac acttctgctg
      721 cggtaaaagc gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa cttattgatc
      781 atcttgatag tttgggtgaa aaaatcatct gggcacccga caaacatctg gggcgttacg
      841 tgcaaaaaca gacgggtgga gacattctat gctggcaggg tgcctgtatt gtgcatgatg
      901 aatttaagac tcaggcgtta acccgcttgc aagaagaata cccggatgct gccatactgg
      961 tgcatccaga atcaccacaa gctattgtcg atatggcgga tgcggtcggt tccaccagtc
     1021 aactgatcgc tgctgcgaaa acattgccac atcagaggct tattgtggca accgatcggg
     1081 gtattttcta caaaatgcag caggcggtgc cagataaaga gttactggaa gcaccaaccg
     1141 caggtgaggg tgcaacctgc cgcagctgcg cgcattgtcc gtggatggcc atgaatggcc
     1201 ttcaggccat cgcagaggca ttagaacagg aaggaagcaa tcacgaggtt catgttgaag
     1261 aaaggctgcg agagagggcg ctggtgccgc tcaatcgtat gctggatttt gcggctacac
     1321 tacgtggata acgaataata aggcgtaacg ttacgctttg ggggaaagat ggattttttt
     1381 agtgtgcaga atatcctggt acatatacca ataggggcag gcggttatga tctctcatgg
     1441 atcgaagcgg taggcacgat cgccgggttg ctgtgtattg gccttgccag tctggagaag
     1501 atcagcaact acttctttgg cctgatcaac gtcaccttgt ttggcattat tttctttcag
     1561 attcagctgt atgccagcct gctattacag gtgtttttct ttgccgcgaa tatttacggt
     1621 tggtatgcgt ggtcgcgaca aaccagtcag aacgaggcgg agttgaaaat tcgctggttg
     1681 ccattgccga aggcactcag ctggttggcg gtttgcgttg tttcgattgg tctgatgacg
     1741 gtatttatta atccggtgtt tgcatttttg acccgcgtgg cagtcatgat catgcaagca
     1801 ttaggattac aggttgtgat gcctgaactg caaccggacg ctttcccgtt ctgggattca
     1861 tgcatgatgg tgttatctat cgtggcaatg attctgatga cgcgtaagta tgtggaaaac
     1921 tggctgttgt gggtgattat taacgtgatt agcgtcgtta tttttgcact tcagggcgtt
     1981 tacgccatgt ctctggagta catcatcctg acctttattg cgctcaacgg cagccggatg
     2041 tggatcaaca gcgcacgtga aagaggctca cgcgcgctgt cccattaatg gtgatgatgt
     2101 gaatggccag acaccccttc attcaggtgg cagtctggcc catgacaagg ttgatattcc
     2161 atctgaatgg tggcgtgctc aatctgatag tgatccatca ggtagtgttg gatctgatcc
     2221 aacaaggcat cgtgatcgtg tggcgggatc acctgcacat gcaacgtcat caccggcttc
     2281 tcgcctacca tccatacatg tacatggtgc acattgcgga cttccgggat ttcccggcac
     2341 atacgacgct tcagttcagc gatatccagc gataccggtg caccttcaag taattcattc
     2401 acgctatctt tcaacagtcg ccacgcgcta cgcagaacca gaagcgacac cagtatcgag
     2461 agaatggggt cagcaggtgt ccagccagtc cagataataa tcaacgcggc aataatagcc
     2521 ccaaccgaac ccagcaggtc cccgagcaca tgcagtgccg ctgctcgtac gttgaggttt
     2581 ttctcttcgc tgccgtgatg aagtaaccag aaagaaagta tatttgccag caacccagcc
     2641 acggcaattg ccatcatcat gccgccctcg accggacgcg gcgtgcggaa gcgttctatc
     2701 gcctcccaga caatcaaaat ggtaattacc acgagggcta tggcattcac aaacgccgcg
     2761 agcgtggtca gtcttagcca accgaaagtg tggcgaatgg tgggaggacg acgtgaaaat
     2821 tgcacggcga gtagggcgaa aagcagggcg gcagtatcgg tcaacatatg acccgcatcg
     2881 gccagcaatg ccagagaacc agaaagaaaa ccaccaacga cttctaccag cataaacccg
     2941 gcagtcacgc cgaaagcata caacaagcga cgagcattat tatcttctgg caggtgtgag
     3001 gacgtgtgtg agtgtgagtg cgccatgacg agtcatccct taattattct cacttaactt
     3061 tatgacatca tactgctttt agaagtgaaa aattaaaagg gagagactcc gctctcccat
     3121 tattggctat tttgcagggt tactgcgtgg taccgtcggt tttggtatcg acatcattat
     3181 tgatgccatc accggtttgt acctttttat tgatatccgg acagcgacca tctttgcaca
     3241 tggtgttctt gtgctcttca tctttggtca ttccgtcatt gttcattgaa gaaccatccg
     3301 aatgcagcat tgtgccgcca gaaccggtat ttaccccgtt attgtcgacg ttatttggcg
     3361 cgacattttc acgggcgtca ggggctacct ggcccgcatc agctgcggcg tttgcctggc
     3421 cgttattagt ttgcgctccg ctatcggcag ccagtgcggc accgctggca aggcttagag
     3481 tggcagtcag aaataatgtg gccagttttg tcattttcat aggatgctcc tgttatggtc
     3541 gttatgtcgg ataacctctt ccaacagtgc atttgcaggt gaatataagg cattggttta
     3601 agatttcagc caggttatga aacgcagcag agaatcttga aataattaac aaacaaagga
     3661 gttacagtta gaaattgtag gagagatctc gtttttcgcg acaatctggc gtttttcttg
     3721 ctaattccag gattaatccg ttcatagtgt aaaaccccgt ttacacattc tgacggaaga
     3781 tatagattgg aagtattgca ttcactaaga taagtatggc aacactggaa cagacatgaa
     3841 ttatcagaac gacgatttac gcatcaaaga aatcaaagag ttacttcctc ctgtcgcatt
     3901 gctggaaaaa ttccccgcta ctgaaaatgc cgcgaatacg gttgcccatg cccgaaaagc
     3961 gatccataag atcctgaaag gtaatgatga tcgcctgttg gttgtgattg gcccatgctc
     4021 aattcatgat cctgtcgcgg caaaagagta tgccactcgc ttgctggcgc tgcgtgaaga
     4081 gctgaaagat gagctggaaa tcgtaatgcg cgtctatttt gaaaagccgc gtaccacggt
     4141 gggctggaaa gggctgatta acgatccgca tatggataat agcttccaga tcaacgacgg
     4201 tctgcgtata gcccgtaaat tgctgcttga tattaacgac agcggtctgc cagcggcagg
     4261 tgagtttctc gatatgatca ccccacaata tctcgctgac ctgatgagct ggggcgcaat
     4321 tggcgcacgt accaccgaat cgcaggtgca ccgcgaactg gcatcagggc tttcttgtcc
     4381 ggtcggcttc aaaaatggca ccgacggtac gattaaagtg gctatcgatg ccattaatgc
     4441 cgccggtgcg ccgcactgct tcctgtccgt aacgaaatgg gggcattcgg cgattgtgaa
     4501 taccagcggt aacggcgatt gccatatcat tctgcgcggc ggtaaagagc ctaactacag
     4561 cgcgaagcac gttgctgaag tgaaagaagg gctgaacaaa gcaggcctgc cagcacaggt
     4621 gatgatcgat ttcagccatg ctaactcgtc caaacaattc aaaaagcaga tggatgtttg
     4681 tgctgacgtt tgccagcaga ttgccggtgg cgaaaaggcc attattggcg tgatggtgga
     4741 aagccatctg gtggaaggca atcagagcct cgagagcggg gagccgctgg cctacggtaa
     4801 gagcatcacc gatgcctgca tcggctggga agataccgat gctctgttac gtcaactggc
     4861 gaatgcagta aaagcgcgtc gcgggtaagg tttaattgtc ggatgcgccg tcagagtggc
     4921 gtatccgatg aatcaccaca ggcctgataa gtcgcgcagc gtcgcatcag gcaatgtgct
     4981 ccattgttag caacaaaaaa gccgactcac ttgcagtcgg ctttctcatt ttaaacgaat
     5041 gacgtttact tcgctttacc ctggtttgca accgccgctg ctttcgctgc gatctcgtca
     5101 gcattaccca gataatagcg tttcagcggt ttgaaattct cgtcgaactc atacaccagc
     5161 ggcacgccag tcgggatatt aagctcaaga atctcttctt cgctcatgtt atcaagatat
     5221 ttcaccagcg cacgtaaaga gttaccgtgt gcagcgatga tcacgcgctc accgctcttc
     5281 atacgcggca gaatagtttc attccagtaa gggatcacgc ggtcaatggt cagcgccagg
     5341 ctttccgtca gcggcagttc tttctcgctc agtttcgcgt aacgcggatc gtgacccgga
     5401 taacgctcat catctttagt cagttccggc ggagtcactg caaaaccacg acgccactgt
     5461 ttcacctgct cgtcgccata cttttcagca gtttccgctt tgttcagacc ctgcaacgca
     5521 ccgtagtgac gttcgttcag tttccaggat ttctcaacgg gcagccatgc ctgatccagt
     5581 tcgtccagca cattccacag ggtatggata gcgcgtttca gcacagaagt gtaagcaaag
     5641 tcaaagctgt aaccttcctc tttcagcagc ttacctgctg cttttgcttc gcttacgcct
     5701 ttctcagaca gatccacgtc gtaccaaccg gtgaaacggt tttctttgtt ccactgactt
     5761 tcgccatgac gaaccagaac cagcttagtt acagccatat acttactcct caaatcatct
     5821 tttaatgata ataattctca ttatattgcc gcgacgaagc aacagcaatg cttacgcata
     5881 accatagcga aaatagtggc gcagtgtaag gttgttgtga atattgagtt gcaaatatgt
     5941 cggtgtttgc tggtgatttg aacaatatga agataaagcc ctcatgacga gggcttaaca
     6001 ttactcagca ataaactgat attccgtcag gctggaatac tcttcgccag gacgcaggaa
     6061 gcagtccggt tgcggccatt cagggtggtt cgggctgtcc ggcagaaact cgctttccaa
     6121 cgccagccct tgccagtcgg cgtaaggttc ggttccccgc gacggtgtgc cgccgaggaa
     6181 gttgccggag tagaattgaa gagccggagc ggtggtgtag accttcagct gcaatttttc
     6241 atctgctgac cagacatgcg ccgccacttt cttgccatcg cctttggcct gtaacaagaa
     6301 tgcgtgatcg taacctttca ctttgcgctg atcgtcgtcg gcaagaaact cactggcgat
     6361 gattttggcg ctgcggaaat caaaagacgt tccggcgaca gatttcaggc cgtcgtgcgg
     6421 aatgccgcct tcatcaaccg gcagatattc gtccgccaga atctgcaact tgtgattgcg
     6481 cacgtcagac tgctcgccgt caagattgaa atagacgtga ttagtcatat tcaccgggca
     6541 aggtttatca actgtggcgc gataagtaat ggagatacgg ttatcgtcgg tcagacgata
     6601 ttgcaccgtt gcgccgagat tacccgggaa gccctgatcg ccatcatctg aactcagggc
     6661 aaacagcacc tgacgatcgt tctggttcac aatctgccag cgacgtttgt cgaacccttc
     6721 cgggccgccg tgcagctggt taacgccctg acttggcgaa agggtcacgg tttcaccgtc
     6781 aaaggtataa cggctattgg cgatgcggtt ggcataacga ccaatagagg cccccagaaa
     6841 cgcggcctga tcctgatagc attccgggct ggcacagccg agcagcgcct cgcggacgct
     6901 gccatcggaa agcggaatac gggctgaaag taaagtcgca ccccagtcca tcagcgtgac
     6961 taccatccct gcgttgttac gcaaagttaa cagtcggtac ggctgaccat cgggtgccag
     7021 tgcgggagtt tcgttcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaaaagtc
     7081 tctttaatac ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc
     7141 agctcttccg ggatcaacgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg
     7201 ccacctttgt cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg
     7261 gtgatttcga aatcatcgcg catagaggca tgagactccg ccatcaactc gcccatacgt
     7321 ttcaggtcgc cctgctccag cgcgctggca gcttcaacgg tgcgggcgtt ttcagtcagg
     7381 atatgccgca cgcgtttcgc cacgatcggg tccagttcat gcgcaacagc gttgaactct
     7441 tcaatggtga catcacgcag ggctggctgc tggaagaaac gcgcaccggt ttcgcactgt
     7501 tcacgacggg tgttgtattc gctgccaacc agggtacgtt tgaagttact gttgatgatg
     7561 acgacagcca cacctttggg catggaaact gctttggtcc ccagtgagcg gcaatcgatc
     7621 agcaaggcat gatctttctt gccgagcgcg gaaattagct gatccatgat cccgcagtta
     7681 cagcctacaa actggttttc tgcttcctga ccgttaagcg cgatttgtgc gccgtccagc
     7741 ggcagatgat aaagctgctg caatacggtt ccgaccgcga cttccagtga agcggaagaa
     7801 cttaacccgg caccctgcgg cacattgccg ctgatcacca tgtccacgcc gccgaagctg
     7861 ttgttacgca gttgcagatg tttcaccacg ccacgaacgt agttagccca ttgatagttt
     7921 tcatgtgcga caatgggcgc atcgagggaa aactcgtcga gctgattttc ataatcggct
     7981 gccatcacgc gaactttacg gtcatcgcgt ggtgcacaac tgatcacggt ttgataatca
     8041 atcgcgcagg gcagaacgaa accgtcgttg tagtcggtgt gttcaccaat caaattcacg
     8101 cggccaggcg cctgaatggt gtgagtggca gggtagccaa atgcgttggc aaacagagat
     8161 tgtgtttttt ctttcagact catttcttac actccggatt cgcgaaaatg gatatcgctg
     8221 actgcgcgca aacgctctgc tgcctgttct gcggtcaggt ctcgctgggt ctctgccagc
     8281 atttcataac caaccataaa tttacgtacg gtggcggagc gcagcagagg cggataaaag
     8341 tgcgcgtgca gctgccagtg ttgattctct tcgccattaa atggcgcgcc gtgccagccc
     8401 atagagtagg ggaaggagca ctggaagagg ttgtcataac gactggtcag ctttttcaac
     8461 gccagcgcca gatcgctgcg ctgggcgtcg gtcaaatcgg tgatccgtaa aacgtgggct
     8521 ttgggcagca gtagcgtttc gaacggccag gcagcccagt aaggcacgac ggctaaccag
     8581 tgttcggttt cgacaacggt acggctaccg tctgccagct cgcgctgaac ataatccacc
     8641 agcattggtg atttctgttc ggcaaaatat tctttttgca ggcggtcttc gcgctcagct
     8701 tcgttaggca ggaagctatt tgcccaaatc tgaccgtgcg gatgcgggtt agagcagccc
     8761 atcgccgcgc ctttgttttc aaaaacctgc acccatgggt acgttttccc cagttctgcg
     8821 gtttgctcct gccaggtttt gacgatttcc gtcaatgctg caacgctgag ctctggcagc
     8881 gttttactgt gatccggtga aaagcagatc acccggctgg tgccgcgcgc gctctggcaa
     8941 cgcatcagcg gatcgtgact ttctggcgca tctggcgtgt cagacatcaa agccgcaaag
     9001 tcattagtga aaacgtaagt cccggtgtaa tcggggtttt tatcgcctgt cacccgcaca
     9061 ttacctgcgc agaggaagca atctggatcg tgcgcaggta acacctgttt ggctggcgtt
     9121 tcctgcgccc cctgccaggg gcgcttagcg cggtgcggtg aaaccagaat ccattgcccg
     9181 gtgagcgggt tgtagcggcg atgtggatga tcaacgggat taaattgcgt catggtcgtt
     9241 ccttaatcgg gatatccctg tggatggcgt gactgccagt gccaggtgtc ctgcgccatt
     9301 tcatcgagtg tgcgcgttac gcgccagttc agttcacggt cggctttgct ggcgtccgcc
     9361 cagtaggccg gaaggtcgcc ctcgcgacgc ggtgcaaaat gataattaac cggtttgccg
     9421 caggctttgc tgaaggcatt aaccacgtcc agcacgctgt tgcctacgcc agcgccgagg
     9481 ttgtagatgt gtacgcctgg cttgttcgcc agtttttcca tcgccacgac gtgaccgtcc
     9541 gccagatcca ttacgtggat gtaatcgcgt acgccagtac catcttcggt cggataatcg
     9601 ttaccaaaaa tcgccagcga gtcgcgacgg cctacagcaa cctgggcgat gtatggcatc
     9661 aggttattcg gaatgccttg cggatcttcg cccatatcgc ccgacggatg cgcgccaacc
     9721 gggttgaagt agcgcagcag ggcaatgctc cagtccggct gggctttttg cagatcggtg
     9781 aggatctgtt ccaccatcag cttgcttttg ccgtaagggc tttgcggtgt gccggtcggg
     9841 aagctttcaa cgtatggaat tttgggctga tcgccataaa cggtggcgga ggagctaaaa
     9901 ataaagtttt tgacgttagc ggcgcgcatg gcgctaatca ggcgcagagt gccgttgaca
     9961 ttgttgtcgt aatattccag cggtttttgt accgattcgc ccacggcttt cagcccggcg
    10021 aagtggatca cggtgtcgat agcgtgatcg tgcaggatct cggtcatcaa cgcttcgtta
    10081 cgaatatcgc cttcaacaaa cgttggatgt ttgccgccta aacgctcgat aacaggcagt
    10141 acgctgcgct tactgttaca gaggttatca agaatgatga catcatgacc gttttgcagt
    10201 aattgcacac aggtatgact tccaatgtaa ccgctaccac cggtaaccag aactctcata
    10261 attcgctcca ttaggcttat ggtatgaaat aaccatagca taacaaagat gcgaaaagtg
    10321 tgacatggaa taaattagtg gaatcgttta cacaagaatt tagccgtttt ttgtgcgcga
    10381 ttaagtgatt ataaaacaga gggtttatga atgattgcgc tttttatctg aaaaaaagac
    10441 gcggtttaat gcctgcatgc gtcgaaccgt tggccggaga gggcgcttag gccgcctccg
    10501 gcaaggtcag cactaccgac tcaatatatt tttgtcagca catagcgata gagtccaccg
    10561 tccggcacga actcaaggcg atgggtaata caggcaggcg catcttcagc gtggtgcgaa
    10621 acaaacaaca attgcgtttc accttcgcta atcagcacat caacaaaacg gcggataagc
    10681 tggcgattga gcggatcaag cccctgtagt ggttcatcga gaataagcaa cgtcggatgt
    10741 ttcaccagtg cgcggacgat cagcgccaga cgctgctgtc cccaggaaag actatggaac
    10801 ggagcgtcag ccgtgcgttt atcaatgccg agaatatcca gccactgctg caccagtttt
    10861 tgctggcgat ccgaaacggc ttgataaatg ccaatcgaat cgaaatagcc agaaagaatc
    10921 acattacgca cggtagtgct gacccggtaa tccagatgca aactactgct gacgtaaccg
    10981 atatgctttt tgatatccca gatggtttcg ccgctgccac gacgtcggcc gaaaagcgtc
    11041 aaatcgttgc tgtaaccttg cggatgatcg ccagtaatca ggcttaataa cgtcgatttt
    11101 cccgcaccat ttggcccgac aatttgccag tgttcgcctg gattcacctg ccagctaagg
    11161 ttattaagaa tggggcgatc gttataagaa accacgccat tgttcagcac aatgcgcggt
    11221 tcgttggcgg gtaaggcgtg acgtgctgaa ggttcatccg gttccggcag ttgcacacct
    11281 tcaagctgtt cgctatgcgc cagttgcgcg acgagtgctt gctggagcag ttcctcttta
    11341 gcgcccgttt ccgctaacgt acaatccgcc agcacgccag caaactggac aaattccggg
    11401 atctcatcga agcgattgag caccagtacc agagtaatac cggactgatg taacgaggcg
    11461 agtcgctcag ccagctgctg acgtgaagca acatccaggc catcgaacgg ctcatcaaga
    11521 atcaacaagt caggctccga catcagcgcc tgacacagca gggtttttcg cgtctcgcca
    11581 gtggaaaggt atttaaagcg tcggtcgagg agggcggtaa taccgaactg ctgcgccagt
    11641 tgcatgcaac gcggtgcatc ctttacttca tcctgaataa tctcagccgt agtgcgtccg
    11701 gtgtcatctt cgccagggcc gagcatatcg gtgttattcc gttgccattc gtcgctgacg
    11761 agcttttgca attgctcgaa ggagagacga gtgatgtggg aaaactggct ttgccgttca
    11821 cctttcaaaa gcggaagttc ccccgccagc gcgcgggcca gggccgattt cccgcttcca
    11881 ttcgaaccga caaacgccca actatcaccc gcgtttaacg ttagctgagg caattgcagc
    11941 gtttttgtgt cgctaagacg aaacgtgcct tgcaaaattt gcaacgatga cattttatat
    12001 cccattttgt gcagcgatta ctgacaggga tacgtgtttc ataacaaatt gtcaacacgc
    12061 ttagcacagc gtggcgataa tcacgctgtc ggcattaaag taggccgtga cattctgccc
    12121 ttgctgaaga gaagtcgctt catttaccgg cactgtggcg cacagtgttt gcccgtcggg
    12181 tagcgccatt aatacttcgc actgctctgc gccgcgctca atatgactaa taatacccgg
    12241 taattggttg tcggcgtttt gcgcgaccgc ctcgtcctga gtaataccta cccacggcgc
    12301 ttttagcaat atcaacactt ctttgccttc atccagcccc agacgcgcgc agctttgtgc
    12361 ggtaattgcg actttcaggc gtgtttttcc gtcagccagt aagacatcaa catgctgttg
    12421 aacgtcatca tgatcgcggg cggtgatggt accgaaccac tggttacggg cgctggtttg
    12481 cagtgaaaaa cgtgagatcg cggccagcag gctgttcagc ggcagggcgt catcgtcact
    12541 taacacatca aaggcttttt gctggatttg cgccagtaag tcatagagct gaatcagtcg
    12601 ctgaccatag cgggtcagta ctgcgccgcc gccaccttta ccgcctgttg cgcgctcgac
    12661 cagaatatgc tcacttaact gattcatctc gttaatggca tcccaggcgc ttttatagct
    12721 aataccggca tctttcgctc cctggctaat ggaaccggaa agcgcaatgt gttttagtag
    12781 cgaaatgcgg cgcgggtcgg cgaataattt ttgttggagc ttaagggtga gaaggatttc
    12841 ggcctgcata acaatgtcct ggcaaaagtc ttattgtgac ggaaaacgaa cgccacgcaa
    12901 agctgaccgc acaaaagggg agtgcttttc tgtgcttagc ggttagaata gtctcatgac
    12961 tatatctgga gttgaccatg ttagagttat taaaaagtct ggtattcgcc gtaatcatgg
    13021 tacctgtcgt gatggccatc atcctgggtc tgatttacgg tcttggtgaa gtattcaaca
    13081 tcttttctgg tgttggtaaa aaagaccagc ccggacaaaa tcattgattc cctgaatgcc
    13141 cgcttagtcg ggcattttct ttttctcaac ttcctgcttt tcctgccgat attttttctt
    13201 atctacctca caaaggttag caataactgc tgggaaaatt ccgagttagt cgttatattg
    13261 tcgcctacat aacgttacat taaggggtta ccaatggctc gtaaatggtt gaacttgttt
    13321 gccggggcgg cactctcttt cgctgttgct ggcaatgcac tggcagatga agggaaaatc
    13381 acggtgttcg ccgccgcatc actgactaac gcaatgcagg acattgctac gcagtttaaa
    13441 aaagagaaag gcgtggatgt ggtttcttct ttcgcttcgt catctactct cgcccgtcag
    13501 attgaagcgg gtgcgcctgc ggatctgttt atttctgccg atcagaaatg gatggattat
    13561 gcggttgata aaaaagcgat cgatacagct acgcgtcaga cactgctcgg caatagcctg
    13621 gtcgttgtag caccgaaagc cagcgtgcag aaagatttca ccatcgacag caaaaccaac
    13681 tggacttcac tgctgaatgg cggtcgcctg gcggttggcg atccggaaca tgttcccgct
    13741 ggcatttatg caaaagaagc actgcaaaaa ctgggcgcat gggatacgct ctctccgaaa
    13801 ctggccccag cggaagatgt tcgtggggcg ctggcgctgg tcgaacgtaa cgaagcgcct
    13861 ctgggcattg tctacggttc tgacgcagtt gccagcaaag gggtaaaagt ggttgccacc
    13921 ttcccggaag attcacataa aaaagtggaa tatccggttg ctgttgtgga agggcataac
    13981 aatgcgacag tgaaagcatt ttatgattat ctgaagggac cgcaggcagc ggaaatcttt
    14041 aaacgttacg gatttacaat caagtaatga tactgaccga tccagaatgg caggcagttt
    14101 tattaagcct gaaagtttct tccctggctg tgctgtttag cctgccgttt gggatctttt
    14161 ttgcctggtt actggtgcgt tgcacgtttc cgggcaaagc tctgctcgac agcgtactgc
    14221 atctaccgct ggtgttaccg cccgtggtcg tcggttactt attattagtt tcgatgggac
    14281 ggcgcggatt tatcggtgaa cgtctgtatg actggtttgg tattaccttc gcctttagct
    14341 ggcgcggcgc ggttctcgct gccgccgtca tgtcgtttcc gctgatggtg cgggcaattc
    14401 gtctggcgct ggaaggggtt gatgtcaaac tggaacaggc cgcaagaaca ctgggggccg
    14461 ggcgctggcg cgttttcttt actatcacgt taccgctgac cttaccggga attattgttg
    14521 gtacggtact ggcttttgct cgttctctcg gtgagtttgg tgcaaccatc acctttgtgt
    14581 cgaacattcc tggtgaaacg cgaaccattc cttctgccat gtataccctg atccagaccc
    14641 ccggcgggga aagtggagcg gcgagactgt gcattatttc tattgcgctg gcgatgatct
    14701 ccctgttgat ttcagaatgg ctggccagaa tcagccgtga acgggcgggg cgctaatcat
    14761 gctggaactg aatttttccc agacgttggg caaccattgc ctgactatta atgaaacgct
    14821 gcccgccaat ggcatcactg ctatctttgg cgtctccggt gccggaaaaa cttcgctgat
    14881 taacgccatc agtggactga cgcgcccgca aaaagggcgg attgtcctca atgggcgggt
    14941 actaaatgat gccgaaaaag gtatctgcct gacgccggaa aagcgtcgcg ttggctatgt
    15001 ttttcaggat gcgcggctgt tcccgcatta caaagtgcgt ggcaatctgc gctacggcat
    15061 gtcgaaaagt atggtcgatc agttcgataa gctggtggcg cttttaggca ttgaaccgtt
    15121 gcttgaccgt ttaccaggca gcctgtccgg aggcgaaaaa cagcgcgtgg cgattggtcg
    15181 ggctttgctg acagcaccgg aattgctgtt gctggatgaa ccgctggcgt cactggatat
    15241 tccgcgtaaa cgcgaactgt tgccttatct gcaacggctg acacgggaaa tcaacattcc
    15301 gatgttgtat gtcagccatt cgctggatga gatcctccat ctggcagaca gagtgatggt
    15361 actggaaaac ggtcaggtga aagcctttgg cgcgctggag gaagtgtggg gcagtagcgt
    15421 gatgaatccg tggctgccga aagagcaaca aagtagcatt ctgaaagtga cggtgcttga
    15481 gcatcatccg cattacgcga tgaccgcgct ggcgctgggc gatcagcatt tgtgggtcaa
    15541 taagctggac gaaccgctgc aagctgcgct acgtatccgc attcaggctt ccgatgtttc
    15601 tctggtttta caaccgccgc agcaaaccag cattcgtaac gtattgcggg caaaagttgt
    15661 taatagttat gacgacaacg gccaggtgga agtggaactg gaagtcggcg gtaaaacgct
    15721 gtgggcgcgt atcagcccgt gggccaggga tgaactggcg atcaaacctg gcctgtggct
    15781 gtacgcgcaa attaaaagtg tgtcgataac cgcctgatta aatcaggtgg ctataaatga
    15841 attgggcaat gctgtcggtg gtgttatcac caatcacaat gttggcgcgc gcttttaccg
    15901 cgtcatcggc gttgcccatc gccacgcctg taccagcggc ttccagcata ctgatatcat
    15961 taaagttatc gccgaatgcc acgacgtttt ccatcgacca accttgcgcc tcaacccatt
    16021 tcgtcaaacg tttacctttg ctgttgccgc cgcgtgcaat atcaacctga tcgtgccagg
    16081 accattcaca ctccagtccc agttcatgtt cgacatgctt accaaaatgc tgcaattgcg
    16141 gcaggtcatc gtgcgtcagg gcgaacttcc atacggcgtt aacttgttgc gccgtttcag
    16201 ccagagaagc gacttgtgtg aaagtcggac gctgttccgg cggcagggtt tgcgcccagt
    16261 tagatgtgcg aatgacatgc ccggtcgggt gctcatagac cattgcatca tcgacataca
    16321 tcagaccgtg aatgtggtgt tcattcagca tctcaatgag ttgcagggct ttaataacgg
    16381 gcattgggtc cgcttccagc acggtttttg catgataatc atacaaatag gtgccattac
    16441 agcaaatagc aggtgtatcc agcgccagcg cctgataaaa aggatgaata gcgacgtgat
    16501 ggcgacctgt gacgatgatt aattgatagc ctgcttcgcg agcgcgggcc agggcttcta
    16561 tcgatgaagg aagcagggtc tttttcgggg tcaataaggt gccgtctaag tcgagagcaa
    16621 tcacgcgtgt ggtcatggcg tattccagat taaggttaag aattttcgtc tgcgcgaatg
    16681 gtacaccgat accactatcg gacaaaattc tgcgttttaa ttcagcattc accgccaaaa
    16741 gcgactaatt ttagctgtta cagtcagttg ctaaatgcaa aggagcattc atgaagcaaa
    16801 cagtttatat cgccagccct gagagccagc aaattcacgt ctggaacctg aatcatgaag
    16861 gcgcactgac gctgacacag gttgtcgatg tgccggggca ggtgcagccg atggtggtca
    16921 gcccggacaa acgttatctc tatgttggtg ttcgccctga gtttcgcgtc ctggcgtatc
    16981 gtatcgcccc ggacgatggc gcactgacct ttgccgcaga gtctgcgctg ccgggtagtc
    17041 cgacgcatat ttccaccgat caccaggggc agtttgtctt tgtaggttct tacaatgcgg
    17101 gtaacgtgag cgtaacgcgt ctggaagatg gcctgccagt gggcgtcgtc gatgtggtcg
    17161 aggggctgga cggttgccat tccgccaata tctcaccgga caaccgtacg ctgtgggttc
    17221 cggcattaaa gcaggatcgc atttgcctgt ttacggtcag cgatgatggt catctcgtgg
    17281 cgcaggaccc tgcggaagtg accaccgttg aaggggccgg cccgcgtcat atggtattcc
    17341 atccaaacga acaatatgcg tattgcgtca atgagttaaa cagctcagtg gatgtctggg
    17401 aactgaaaga tccgcacggt aatatcgaat gtgtccagac gctggatatg atgccggaaa
    17461 acttctccga tacccgttgg gcggctgata ttcatatcac cccggatggg cgtcacttat
    17521 atgcctgtga ccgtactgcc agcctgatta ctgtcttcag cgtttcggaa gatggcagcg
    17581 tgttgagtaa agaaggcttc cagccaacgg aaacccagcc gcgcggcttc aatgttgatc
    17641 acagcggcaa gtatctgatt gccgctgggc aaaaatctca ccacatctcg gtatacgaaa
    17701 ttgttggcga gcaggggcta ctgcatgaaa aaggccgcta tgcggtcggg cagggaccaa
    17761 tgtgggtggt ggttaacgca cactaaccgc tgatttaccc ggcgcagtct ctcctgcgcc
    17821 ggtgtattaa cctatctcct gtaacgcgtg tctctggcgt tcgacgatat tggtccacaa
    17881 attgtcttta tcgtcagtcc ataaattaat cagcaaggca aaaaagcgtt ctgctgctgg
    17941 agaaagtacg gcatctttac ggcgaattat tcccaatgtt cgacgtataa cgggttccat
    18001 caatgggata ccaataatcg aagaataggg cgcatgaggc attgccaggc caggaagcgc
    18061 cgaaatcccc agtcccgcct ccaccagtcc taatgacgtc gaaagatgac gcacttcgta
    18121 aaaccagtcc agcttccagg gcttgtcggc cagctgttgt tctatcaaca gtcggttgcc
    18181 gctggaggag cgtacgccaa tcattttgta acccactaac tcctgccatt ctacaagctg
    18241 ctttttggcc aatggatggt cacgccgaca ggccagcacg aacggctcgt tgaccagtgg
    18301 ggtaaaatca atggatgaat ttgtgacgtt gttcatgttt atgccaaagt ctgattcgtt
    18361 acacagcacc gattccatgc aattattcgt gccttgttcc agaatccgca ctttaatatt
    18421 gggatacagc tcattaaatt taccgattgc cagcggtaaa aaataaaata ctgcggtcgg
    18481 aatacacgct aacgtcacca taccacgatg ataagcattc atatcacgaa tattaaaaag
    18541 cgtttcatcg aatttcttta ttaattccgg cgcctccggg agcaaccttt ttcctgcttt
    18601 tgttaacgta accttgcgag ttgtacgctc aaatagttga atatgtaaat cttcctccat
    18661 cttttttatt cttcgcgtta atgcaggttg cgtaatattg agtaatttag cggcattgtt
    18721 aaacgaactg gactctgcca gtatgacaaa tgccttcata cttgataatt catgcttcat
    18781 tattactccg gaaaatggaa gcgacgattt tgggtggctg gccgttaaaa attttaactg
    18841 catttagcca acttaaatta atgaaaaaat gttattaatc gttgagctaa agtcattaga
    18901 gatgctttgc ccttaatgta accatatcgc aataagttat gtttttaaat tgagggcatt
    18961 attatgaaaa aaataccctg cgtgatgatg cgaggtggaa cctcgagggg cgcgttcctg
    19021 ttagcggaac atttacccga agatcaaacg cagcgcgata aaatattgat ggcaattatg
    19081 ggttccggta acgatctgga aattgacggt attggcggcg gtaatccact gaccagtaaa
    19141 gtcgccatta ttagccgttc cagcgatccg cgtgctgatg tcgattatct gtttgcgcag
    19201 gtaatcgttc atgagcaacg tgtcgatacc acgcctaact gcggcaatat gctgtctggt
    19261 gttggggcat tcgccattga aaatggtttg attgcagcga cttcgccagt tactcgcgta
    19321 cgtatccgca acgtcaatac gggtacgttc atcgaagctg atgtgcaaac gccaaatggt
    19381 gttgtcgagt acgagggtag cgccagaatt gacggcgtac cgggtactgc cgcaccggtt
    19441 gcgctcactt tcctgaatgc cgctggaacc aaaaccggaa aagttttccc gactgataat
    19501 cagattgatt attttgacga tgtcccggtg acctgtatcg atatggcgat gccagtcgtc
    19561 attattccgg ctgaatatct gggaaaaaca ggttatgaat taccggcgga actggatgcc
    19621 gacaaagcat tattagcccg cattgaatct atccgtctac aagcgggtaa agcaatgggc
    19681 ttaggtgatg tcagtaatat ggttatccct aaacctgtgc ttatttctcc agcgcagaaa
    19741 ggcggggcaa ttaatgtgcg ttattttatg ccgcattctt gccatcgcgc gctggcgata
    19801 accggtgcta ttgctatttc cagtagttgt gcattggaag gcaccgtcac ccgacaaatc
    19861 gtcccttctg taggatacgg caatatcaat attgaacacc ccagtggtgc gctcgacgtt
    19921 catttaagta atgaaggtca ggatgccacg acgttacgcg catctgttat tcggacgacc
    19981 agaaaaatat tttccggtga agtttatctt ccctgaaaaa attcgttgtc aggataagga
    20041 caatcaataa aggacttctg tatgagtcat acagaaagaa caggatttta aatgaataag
    20101 aaatcgttat ggaagctaat tctgatatta gcgatcccat gtattattgg ctttatgcca
    20161 gctccggcag gattaagcga actggcgtgg gtgctttttg gtatttacct ggcggccatt
    20221 gtggggctgg ttatcaagcc tttcccggaa cctgtcgtac tgttaattgc cgttgctgcc
    20281 tcaatggtgg tggtcggtaa cttatccgac ggtgcgttta aaaccaccgc cgtattaagc
    20341 ggttactctt caggtaccac ctggctggtg ttctcggcgt ttaccttaag cgccgcattt
    20401 gtgaccaccg gtttaggtaa acgtattgcc tatctgctga ttggtaaaat cggtaacacc
    20461 acgctgggtc tgggttacgt tacggtattc ctcgatctgg tactggctcc ggcaacaccg
    20521 tctaacaccg cgcgtgcggg cggcattgtg ttaccgatca tcaacagcgt ggcggtggct
    20581 ttggggtccg aaccggaaaa aagtccgcgt cgtgtcggac attacctgat gatgtccatt
    20641 tacatggtca ccaaaaccac cagctatatg ttctttaccg caatggcggg gaacattctg
    20701 gcgctgaaaa tgatcaacga cattctgcac ctgcaaatta gctggggtgg atgggcgctg
    20761 gcagccggat tgccgggcat cattatgctg ctggtcaccc cgctggtgat ttacaccatg
    20821 tatccaccag aaattaagaa ggtggataac aaaaccatcg ctaaagcggg ccttgccgaa
    20881 ctaggaccga tgaaaatccg cgaaaaaatg ctgctcggtg tctttgtgct ggcgctgctg
    20941 ggctggattt tcagtaagtc tctgggggtt gatgaatcca ccgtggcaat cgttgttatg
    21001 gcaaccatgc tgctgctggg tatcgttacc tgggaagacg tggttaaaaa taaaggcggc
    21061 tggaatacct taatctggta cggcggtatt atcggcttaa gctccttatt atcgaaagtt
    21121 aaattcttcg aatggttagc tgaa
//
LOCUS       DACAPB010000068        20967 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.068, whole genome shotgun sequence.
ACCESSION   DACAPB010000068 DACAPB010000000
VERSION     DACAPB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.068"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..980)
                     /locus_tag="IT904_002272"
     CDS             complement(<1..980)
                     /locus_tag="IT904_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP3192045.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWG"
     gene            1193..2656
                     /gene="bepA"
                     /locus_tag="IT904_002273"
     CDS             1193..2656
                     /gene="bepA"
                     /locus_tag="IT904_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAP3192046.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            2677..3036
                     /gene="arsC"
                     /locus_tag="IT904_002274"
     CDS             2677..3036
                     /gene="arsC"
                     /locus_tag="IT904_002274"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAP3192047.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENSVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3174..3920)
                     /gene="hda"
                     /locus_tag="IT904_002275"
     CDS             complement(3174..3920)
                     /gene="hda"
                     /locus_tag="IT904_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAP3192048.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3970..5259)
                     /gene="uraA"
                     /locus_tag="IT904_002276"
     CDS             complement(3970..5259)
                     /gene="uraA"
                     /locus_tag="IT904_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP3192049.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5345..5971)
                     /gene="upp"
                     /locus_tag="IT904_002277"
     CDS             complement(5345..5971)
                     /gene="upp"
                     /locus_tag="IT904_002277"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP3192050.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6296..7333
                     /gene="purM"
                     /locus_tag="IT904_002278"
     CDS             6296..7333
                     /gene="purM"
                     /locus_tag="IT904_002278"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP3192051.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7333..7971
                     /gene="purN"
                     /locus_tag="IT904_002279"
     CDS             7333..7971
                     /gene="purN"
                     /locus_tag="IT904_002279"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP3192052.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKVKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8141..10207
                     /gene="ppk1"
                     /locus_tag="IT904_002280"
     CDS             8141..10207
                     /gene="ppk1"
                     /locus_tag="IT904_002280"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAP3192053.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10212..11753
                     /gene="ppx"
                     /locus_tag="IT904_002281"
     CDS             10212..11753
                     /gene="ppx"
                     /locus_tag="IT904_002281"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAP3192054.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGKNFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11792..14035)
                     /gene="pdeF"
                     /locus_tag="IT904_002282"
     CDS             complement(11792..14035)
                     /gene="pdeF"
                     /locus_tag="IT904_002282"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAP3192055.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14217..14369)
                     /locus_tag="IT904_002283"
     CDS             complement(14217..14369)
                     /locus_tag="IT904_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192056.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14387..14578
                     /locus_tag="IT904_002284"
     CDS             14387..14578
                     /locus_tag="IT904_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAP3192057.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            14889..15407
                     /locus_tag="IT904_002285"
     CDS             14889..15407
                     /locus_tag="IT904_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP3192058.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15423..15962
                     /locus_tag="IT904_002286"
     CDS             15423..15962
                     /locus_tag="IT904_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192059.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16260..18212)
                     /locus_tag="IT904_002287"
     CDS             complement(16260..18212)
                     /locus_tag="IT904_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP3192060.1"
                     /translation="MSKKELKPELLTVGKLFTDNYLIPIYQRNYAWRAEQIEQLISDI
                     QDSVVGGQDNYFLGNLVVIKRGREDEFEVIDGQQRLTTLYLLLTFLEQDGEGEKPSVG
                     HAGHLQYESRARATEALLRVAQEAAKEHVRPQGSTSNADAGIHEGYSIINQFFKQNEN
                     LNRSREKFSDFLLTKVTVVRASLPPNTDLNRYFEIMNTRGQQLKQVDIVKARLMSKLP
                     NQYERECFAWVWDSCADMDSYVQMSLTRGDTSLRNKVFGDEWSWLEVTSFSSLMESRP
                     QSGINSSRQSSEGVSLSLDEALSKYAKELESNSTEDEGNERFRSTIEFPAFLLHVLRI
                     MKGDEVEDEGLLDDKRLIKSFDDAVNNVPDAKADWVRSFAFMLLKCRNLFDGFILKRQ
                     FTANIGDDGDWSLQRLKKGGGDKKPTPTYIHVFSASNGSLEEDGSADPHTRDVLLLQS
                     MLRITYTSPRTMHWITKVLRWLSVKAPRDVKHADLADLLKGYARSKVKETFSFEKDQQ
                     PQGFGISRIVFSYLDYLLLSDSSKRDFKFQFRTSIEHFYPQHPDKEQSGAVVSGSSLN
                     LLGNLALVSVSANSKFSNSLPRAKAENFKDTIELQSPKLKRMAEITRNTNWDDQQITA
                     HHEAMVTLLRDDVSLVEASGRSNPGR"
     gene            complement(18209..19489)
                     /locus_tag="IT904_002288"
     CDS             complement(18209..19489)
                     /locus_tag="IT904_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP3192061.1"
                     /translation="MAINGENGVSAVTVDELLKQGLRIPNYQRPYSWDVSTALQLVDD
                     ISEALRDTERKDIPYVLGAIILHDDGEYLNVVDGQQRLLTLRMILAALDPINHQISMS
                     GNSETPVSLVWIELQRRLSQLEDKKEFLDFICHKCQLVRIVTDDIDEAFRVFDSQNYR
                     GKPLAPHDLLKAHHLREMHDESAAMKVAVVEAWEAVNDEDLDRLFSTFLYRISKWSRG
                     ESSLEFTIRDIGMFKGISSRSHRSFSPNLRYHLAAQAAMPLLSAWSVSSTHDARNAGR
                     SRFQLDAPIIAGRSFFEMVTFMLDELKILEQEVIDRGFKNFGPSQSRYRYVYELFIAA
                     LLCYTNKFGDEDVDEVRNRLFAWAYALRVELLRVQFVSADNRARGKNDANKSPFVLLR
                     NAMTGSVVRKLPITSKPYSDNHEKELVAFIKGLQ"
     gene            complement(19872..>20967)
                     /gene="guaA"
                     /locus_tag="IT904_002289"
     CDS             complement(19872..>20967)
                     /gene="guaA"
                     /locus_tag="IT904_002289"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP3192062.1"
                     /translation="SCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCE
                     ALWTPAKIIDDAVARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNG
                     LLRLNEAEQVLDMFGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDE
                     EALKLEDVKWLAQGTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKEL
                     FKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRK
                     ADLYDKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGR
                     VSNRIINEVNGISRVVYDISGKPPATIEWE"
BASE COUNT         5576 a         5285 c         5073 g         5033 t
ORIGIN      
        1 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
       61 aggttaaccg cttcggagaa caacaccggt actaacaggt tgccgtccag cgcctgaata
      121 atcagataca ctgcgaagca gctccagaat tccgtgcctg caccaaactg gaatagcgcc
      181 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag taccgagaag
      241 ccgaccagca ccgccagcag cagcgaatag ttcagcccaa agagcaagaa ccccagccag
      301 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
      361 tgattcatct ccttccacac ctgtcctgcc agtccacggt tgcgcggcag cacccgacga
      421 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
      481 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
      541 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
      601 gcatccatta acgccggata gcggcgaggc aacgtggcgg caaagtcaga aagcttattg
      661 agcatccccg gcatatcgcg aattaagtag atgccctgtt gccaggcgat aggcagtacc
      721 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
      781 cggcgggagc agccaataga ttgcaggcgc acagttggcc attccagcaa ataggccagc
      841 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
      901 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
      961 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
     1021 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactga
     1081 aaagtcattt gagtgggcta atcttcgccg ttacactcaa atgcggcgcg gtgggaacga
     1141 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
     1201 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
     1261 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
     1321 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
     1381 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
     1441 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
     1501 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
     1561 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
     1621 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
     1681 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
     1741 cggtacactg gcgggaacac gtcaggggat gatcagtttc acccagcaaa atgaacagga
     1801 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccgc aggcgatgcc
     1861 aaccttcctc gaaaaattac tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
     1921 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
     1981 gcgaccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
     2041 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
     2101 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
     2161 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
     2221 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
     2281 caatcgcctg aaaaatgccc gtgatttgcg caccaatccg gtgttgcagc tcaacctggc
     2341 aaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctca atcgctacac
     2401 ctttaataat aaagatgaca gcaacggctg ggatttactg gcacaggcgg aagccgcgct
     2461 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
     2521 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtgaaattag gcagcctgca
     2581 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
     2641 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
     2701 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacagcgtg
     2761 gagccagaag tggtactcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
     2821 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
     2881 aaagaactga acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
     2941 gataatccga agctaatgga gcgcccgatt gtggtcgcga atggcaaagc gcggattggt
     3001 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
     3061 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
     3121 ttcgcgtagt tcggataagg cgttcgcgcc gcatccgaca ataaacacct tatctacaac
     3181 ttcagaattt ctttcacaaa cggaatggtc agcttacgtt gcgcggtaat cgacgcacga
     3241 tccaactgat ccaacgtcat aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag
     3301 aaacgcccca catcttccgg cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc
     3361 tgcaacttat cttcatcaga aagtggctgc aatttgtaga tctgccccca gtcgagtcgc
     3421 gacgcgagat ccggtaatcc cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac
     3481 aatcgtgttt tgcccgattc cagaattcga ttgtagagat cgaaaatcgc catctcccac
     3541 aactcatcgc ctgcaataca ctcaatgttg tcgatacaga ccagcgacaa atgctccata
     3601 ccgtcgagca cttccggaac aaaccaggtg cgtttatcca gcgggacata gcccacagca
     3661 tcgccacgct gcgacaattc cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct
     3721 tcgcgtgccc agagatagat gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgcg
     3781 gccagtaaag aggagttatc ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga
     3841 taaagtggca aagagagctg tgccggtgtg ttcagagata cctcaaccag gatttcacaa
     3901 aatcgcgaga agtttaccac agaatcccat gatgtttgaa ccgggcagca acactgcccg
     3961 gtcggtacat tatttgtctg ttatatccgc gtcttctgcg tccagcacca cttcttcagg
     4021 gcgcagcaca ctgatcagtt tgaagatcaa acttaagccg ataccgacga tggtcgccag
     4081 cgccatacct ttcaactccg ccgcgccgat gtttaccttc gcgccactga cgccgatgat
     4141 caaaatcacg gaagtcagga tcaggttctg tgctttgttg taatccactt tcgattcgat
     4201 caaaacacga ataccggaag caccgatgac accataaagc agcagcgaaa cgccgcccat
     4261 caccggcaat gggatcatct ggatagcggc agccagttta ccgacgcagg aaagcaggat
     4321 agcgaaaatc gccgccccgc cgataaccca agtactgtaa acacgggtga tcgccatcac
     4381 gccaatgttt tctccgtaag tagtatttgg cgtagagcca aagaagccgg agatcacagt
     4441 cgacaggccg ttagcaaaca tcgaacggtg cagacctgga tcgcgcagca gatctttttt
     4501 gacgatatta gccgttacta ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc
     4561 tggcagaata gtcagaatgg caaaccactc gaagcgcggc gtatagaggg ttggcagcgc
     4621 aaaccagtga gcattaataa tcggcgtggt atcgacaatt cccattgcga aagagagcgc
     4681 gtaccccacc agcacgccaa ttaaaatcgg gataattgcc aggaaaccac ggaacagcac
     4741 ggaacctaaa accgtgaccg ccagggtggt aatagagatg atgatggttt tggagtctgg
     4801 cgtttgccct tcagccggga gtaaacccgc cataccggca gctacgcccg ccagctccag
     4861 accgatgacg gcaacgattg cgcccattgc cgcaggtgga aacagcacgt ccagccagcc
     4921 ggtccccgct ttcttcacga taaaagaaac caggcagaac agcacgccgc acataataaa
     4981 gccgcccagc gcgacttcat accctaacgg caacagtaac aataccggtg aaataaaggc
     5041 aaagctggaa ccaagataag ccggaatttt ccctttacag atgaagagat acagcagcgt
     5101 tccaataccg ttaaataaca gtacagtcgc cgggttaata tgaaataaga cgggcaccag
     5161 gacggttgca ccaaacatgg cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag
     5221 tggcggtctt tcactcaccc cgatagcacg gcgcgtcata gtattatcct ctgtattatg
     5281 tgttataggc gctttactca aaaaaaagcc gactcttaaa gtcggcttta attattttta
     5341 ttctttattt cgtaccaaag attttgtcac cggcatcgcc gaggcccgga ataatgtatc
     5401 cgtgctcgtt cagtccctga tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt
     5461 tttccagcgc agcgatacct tctggcgcag cgaccagcac cagaactttg atgctgctgc
     5521 agcccgcttt tttcagcagg tcgatggtcg cgataacgga accaccggtt gccagcattg
     5581 ggtcaacgat cagcgccata cgctcatcga tgttagaaac cagtttctgg aagtacggta
     5641 ccggctccag cgtttcttca ttacggtaca taccgacaac gctgatgcgc gcgctcggaa
     5701 cgttttccag cacaccgtcc atcataccaa gacccgcacg cagaattggc acaacggtaa
     5761 ttttcttacc tttgatctgg tcgatttcta ccgggccgtt ccagccttcg atagttactt
     5821 tttccgtttc gaggtcggcg gtcgcttcgt aagtcagcag gctacccact tcggaagcga
     5881 gttcgcgaaa gcgcttggtg ctgatatctt gctcacgcat cagtcccagc ttgtgtttga
     5941 cgagtgggtg tttgacttcc acgatcttca tactctttct cctttgaggg gcagccacaa
     6001 aaaaaatcga cggattatac ctcctttctt caaggcggca atattctttt cgttgacttt
     6061 agtcaaaatg ataacggttt gagataaagt tattttatat tcaaatggtt atgaaagaag
     6121 attattccat ccgaaaacta acctttaccc tggtacaagt cttctttcgc cgcgcgcctg
     6181 ggcaaaagac gtgcaaaaag gttgtgtaaa gcagtctcgc aaacgtttgc tttccctgtt
     6241 agaattgcgc cgaattttat ttttctaccg caagtaacgc gtggggaccc aagcagtgac
     6301 cgataaaacc tctcttagct acaaagatgc cggtgttgat attgacgcgg gtaatgctct
     6361 ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct
     6421 gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc
     6481 cggcactgac ggcgtaggta ccaagctgcg tctggcaatg gacttaaaac gtcacgacac
     6541 cattggtatt gatctggtcg ccatgtgcgt taatgacctg gtggtgcaag gtgcagagcc
     6601 gctgtttttc ctcgactatt acgcaaccgg aaaactggat gttgataccg cttcagcggt
     6661 gatcagcggt atcgcggaag gatgtctgca atcgggctgt tcactggtgg gtggcgaaac
     6721 ggcagaaatg ccggggatgt atcacggtga ggattacgat gtcgcgggtt tctgcgttgg
     6781 cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat
     6841 tgcactcggt tccagcggtc cgcactcgaa cggctattcg ctggtgcgca aaattcttga
     6901 agtcagcggt tgtgatccgc aaaccaccga acttgatggt aagccattag ccgatcatct
     6961 gctggcaccg acccgcattt acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt
     7021 gcatgccatt gcgcacctga ccggcggcgg cttctgggaa aacattccac gcgtattgcc
     7081 agataatact caggcagtga ttgatgaatc ttcctggcag tggccggaag tgttcaactg
     7141 gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt
     7201 cgggatgatt atcgccctgc ctgctccgga agtggacaaa gccctcgccc tgctaaacgc
     7261 caacggtgaa aacgcgtgga aaatcggtat catcaaagcc tctgattccg aacaacgcgt
     7321 ggttatcgaa taatgaatat tgtggtgctt atttccggca acggaagtaa tttacaggca
     7381 attattgacg cctgtaaaac taacaaagtt aaaggcaccg tacgggcagt tttcagcaat
     7441 aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc
     7501 atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg agttgattca tgaaatcgac
     7561 atgtacgcac ccgatgtggt cgtgctggct ggttttatgc gcattctcag cccggcgttt
     7621 gtctcccact atgccgggcg tttgctgaac attcaccctt ctctgctgcc gaaatatccc
     7681 ggattacaca cccatcgtca ggcgctggaa aatggtgatg aagagcacgg tacatcggtg
     7741 catttcgtca ccgatgaact ggacggtggc ccggttattt tacaggcgaa agtcccggta
     7801 tttgctggtg atacggaaga tgacatcacc gcccgcgtgc aaacccagga acacgctatt
     7861 tatccactgg tgattagctg gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg
     7921 tggctggatg gtcaacgtct gccgccgcag ggctacgctg ccgacgagta atgccccgta
     7981 gttaaagcgc cagctctgcc gctggcgttt ttcaattcac ctgtaaatcg caagctccag
     8041 cagttttttt ccccttttct ggcatagttg gacatctgcc aatattgctc gccataatat
     8101 ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta atgggtcagg aaaagctata
     8161 catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga
     8221 caaatctaac ccgctgattg aaaggatgcg tttcctgggg atctattcca ataaccttga
     8281 tgagttctat aaagtccgct tcgctgaact gaagcgacgc atcattatta gcgaagaaca
     8341 aggctccaac tctcattccc gccatttact gggcaaaatt cagtcccggg tgctgaaagc
     8401 cgatcaggaa ttcgacggcc tctacaacga gctgctgctg gagatggcgc gtaaccagat
     8461 cttcctgata aatgaacgcc agctctccgt caatcaacaa aactggcttc gtcattattt
     8521 taagcagtat ctgcgtcagc acattacgcc gattttaatc aatcctgaca ctgacttagt
     8581 gcagttcctg aaggatgatt acacctatct ggcggtggaa attatccgtg gtgataccat
     8641 ccgttacgcg ctgctggaga tcccatcaga taaagtgccg cgctttgtga atttaccgcc
     8701 agaagcgccg cgtcgacgca aaccgatgat tcttctggat aacattctgc gttactgcct
     8761 tgatgatatt ttcaaaggct tctttgatta tgacgcgctg aatgcctatt caatgaagat
     8821 gacccgcgat gccgaatacg atttagtgca tgagatggaa gccagcctga tggagttgat
     8881 gtcttccagt ctcaagcagc gtttaactgc tgagccggtg cgttttgttt atcagcgcga
     8941 tatgcccaat gcgctggttg aagtgttacg cgaaaaactg actatttccc gctacgactc
     9001 catcgtcccc ggcggtcgtt atcataattt taaagacttt attaatttcc ccaatgtcgg
     9061 caaagccaat ctggtgaaca aaccactgcc gcgtttacgc cacatttggt ttgataaagc
     9121 ccagttccgc aatggttttg atgccattcg cgaacgcgat gtgttgctct attatcctta
     9181 tcacaccttt gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc cgagcgtact
     9241 ggcgattaaa attaacattt accgcgtggc gaaagattca cgcatcatcg actcgatgat
     9301 ccacgccgca cataacggta agaaagtcac tgtggtggtt gagttacagg cgcgtttcga
     9361 cgaagaagcc aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt
     9421 ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg atttcacgta aagaaaacgg
     9481 tgaagtggtc cgttacgcac acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct
     9541 ttatactgac tattcgttgc tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt
     9601 atttaacttt attgaaaacc cataccgccc agtgacattt gattatttaa tggtgtcacc
     9661 gcaaaactct cgccgtctgt tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca
     9721 agggctgccc agtggtatca ccctgaagct aaataacctt gtcgataaag gcctggttga
     9781 tcgtctgtat gcggcctcca gctccggcgt accggttaat ctgctggttc gcggaatgtg
     9841 ttcgctgatc cccaatctgg aaggcattag cgacaacatt cgtgccatca gtattgttga
     9901 ccgttacctt gaacatgacc gggtttatat ttttgaaaat ggcggcgata aaaaggtcta
     9961 cctttcttcc gccgactgga tgacgcgcaa tattgattat cgtattgaag tggcgacacc
    10021 gctgctcgat ccgcgcctga agcagcgggt gctggacatc atcgacatat tgttcagcga
    10081 tacggtcaaa gcacgttata tcgataaaga actcagtaat cgctacgttc cccgcggcaa
    10141 tcgccgcaaa gtacgggcgc agttggcgat ttacgactac atcaaatcac tcgaacaacc
    10201 tgaataaccc tatgccaata cacgataaat cccctcgtcc gcaggagttt gctgcggtcg
    10261 atcttggttc aaacagtttt cacatggtca tagcccgtgt ggtagatggt gccatgcaga
    10321 ttattggccg cctgaaacag cgggtgcatc tggcggacgg cctggggcca gataatatgt
    10381 tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc gaacggctac
    10441 aagggttttc tcctgccagc gtctgtatag tcggcaccca tacgctgcgt caggcgctga
    10501 acgccactga ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt gaaattattt
    10561 ccggtaatga agaagcccgt ctgattttta tgggcgtgga acatacccaa ccggagaaag
    10621 gtcgcaaact ggttattgat attggcggcg gatctacgga actggtgatt ggtaaaaatt
    10681 tcgaaccgat tctcgttgaa agccgccgga tgggttgtgt cagctttgcc cagctttact
    10741 tccctggcgg ggtcatcaat aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa
    10801 aactggaaac tttaacctgg caattccgta ttcagggctg gaacgtggca atgggcgctt
    10861 ccggtaccat aaaagccgcc catgaagtgt taatggaaat gggcgagaaa gacgggataa
    10921 ttaccccgga acgtctggaa aaactggtaa aagaagtttt acgtcaccgt aatttcgcat
    10981 cgctgagttt accgggtctt tccgaagagc ggaaaacagt cttcgttcct ggactggcga
    11041 ttttatgcgg tgtgtttgat gctttagcca tccgtgaact gcgcctttct gacggggcgt
    11101 tacgcgaagg cgtactgtat gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc
    11161 gcaccgccag cagcctcgcc aaccagtatc acatcgacag cgagcaggcc cgacgggtgc
    11221 tggataccac tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc
    11281 cgcaactgga ggcgctactg cgatgggccg ccatgctgca tgaggtcggg ttgaatatca
    11341 accacagcgg tttgcatcgc cactccgctt atattctgca aaacagtgac ttgccgggtt
    11401 ttaatcagga acagcagctg atgatggcga cactggtgcg ctatcaccgt aaagcgatta
    11461 agctcgacga tctgccgcgc tttaccttgt ttaagaagaa acagttcctg ccactgatac
    11521 agctattgcg ccttggcgta ttactaaaca atcaacgtca ggcaaccacc acaccgccaa
    11581 cattgacact gattactgat gacagtcact ggacactgcg tttcccgcat gactggttta
    11641 gtcagaatgc gctggtactg cttgatctgg aaaaggagca agaatactgg gaaggcgtgg
    11701 ctggctggcg gttgaaaatt gaagaagaaa gtacacctga aatcgccgct taatgctttg
    11761 cgggccgacg agaaatgtcg gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt
    11821 cattcagcgt atcaattaac ggctgcggct taccaataag ataaccctgc atataatcga
    11881 tccccaaaga gagcaccgcc tcgcggatct cttcgttttc aacgtactct gccactaccc
    11941 gcattttctt cattcgcgcc aggtggcaaa tcgacgccac tatctgataa tccagactat
    12001 ttgacacaat attgcggata aagctgccgt caattttaag cagatcggca ttcacatttt
    12061 taagccgtgc atagctggcg tagccggtgc cgaaatcatc aatcgcaatc tggcagccta
    12121 attcctgaag atgctgcaag gtgatttgcg cctgcttaac attggtcaga gcattacttt
    12181 cggtgacttc aaaaataagt tgccacgctt caatctgata tttagccagc aactgactga
    12241 cttcaacagg aaaacgagcc tgacataccg aggttggaga cagattaata gcaaaacggt
    12301 gagcgggcat cttcgctctg ttttcagcca taaattgcag cgtatgctca atgacccaca
    12361 tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg caagaagcta tcggggctga
    12421 tcagttcatc attctcacct ttcatgcgca gaagaatttc atggtaaaca tcaccacgca
    12481 taccggtaat cggctgggcc atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc
    12541 gattcatcat cgcgacttta tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct
    12601 gcatattttc cggggcgttg gtcacgatgg aaagttcggc gaccgtattt agctctccca
    12661 gcagcaggta gatatgattc actggcgagc gcacatagca gtaactgacg ccaatctgcg
    12721 gttgcatcgg catgccatcc caaaagaaac gaaattgctt gagatggcta tccagtgcgg
    12781 taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta cccgaaagct
    12841 gataaacatc ttcacccggt tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt
    12901 attgaatccg cagcatgatg ccatagttct taaccagcat ttccatgcca gggatgcgta
    12961 aataacaaag cgcagaccag ggggcatcac gcaacgcgcg attcagggcg cgaacatttg
    13021 gcagatgaac caccgggtcc acatacgcaa gccgctgaat gcgtctgact accgctctct
    13081 gacgggttgc cagtacagcc atataattga caataaaaga gaataccaga taactggagg
    13141 aggttatggt cagctgcgtg gtatagccag gataaatggg aatgtaattt tgatagctgt
    13201 ggatgctgat catcaacacg accgcccaga gcagcgaaat cagcttataa ccatagcgca
    13261 tcgctcccca catcatcagg ggcagcaata atgacaaggt ataattggtg ctaaaaattg
    13321 tgcttttttc atttaacggc atgcataaca gcaacaataa agcacctaat gccagtagcc
    13381 agagcgcgaa ctcttttttg gtgactttgg catcaacctg ctgttttaat tgcgaataat
    13441 agctacgtaa ataaaatgga tttcgcacta cccgaatgat gaagtagcac agcgggacac
    13501 cgatcagatt acccaccagt aaggcctgat aattgattaa agtcccgagg ttaaaaggca
    13561 tgacaccgac cagattttct ctgctcgcca gtaatcctac aaatgcagca aactggaaaa
    13621 gtatcagaaa cagcgttgca ggaaacacaa tctgccagaa tatacgctgg gaaattaaac
    13681 gggtatcacc atgtgaaacg ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac
    13741 agagaatgat aatgataata aaattagccg ttaatgatag cgtttcgtaa aaacccagct
    13801 ctgcatattt gcggacaaaa atccctaagg cgatccctgg caatgccgcc cagctgaaaa
    13861 acatcatcat gctgatcatc agtgccagag gcagataaaa gagaaaaacc tcaccggaag
    13921 aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca
    13981 gcggaagccc ccaccattta tcacgtattt ttatataagt tgcattcagt ttcatagatg
    14041 ctcagcagaa tcccccacat cctgaaggag gtgtattcag acaggcatcc cacctgactt
    14101 cgaatgatga ttattcatca ctatagagag cattgattct aagtgtcata tgaaagtacc
    14161 aattgatata tatcaaacaa aataaccctg attaatgaat tattacgttt atcatgttaa
    14221 ttcatcatta ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat
    14281 gtatccactg acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta
    14341 atgtaagtac cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac
    14401 cagtatgcga aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag
    14461 ctttatcttc ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca
    14521 gagcaaaaaa gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg
    14581 gttaccttct ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctgtt
    14641 tgggattatc aaagcggcag atattctttc atcttaaatt ttacgtcttt atcctgactg
    14701 atgtttatcc tgtttggctg cgaaataaat ataaaattaa tatatatgtt gtaatgatat
    14761 atttttataa attattccct gcgtgaattt taataaattt aatctatccc tttatacgca
    14821 atacatttac tttcctcttt tgatgatctt aaatgtctta tttttcgtaa tgtgtataac
    14881 aaggaatagt gatgaaattt aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca
    14941 ctctggcagg atgccagtca aatgctgacg atcatgctgc cgatgtttat caaaccgatc
    15001 aactgaatac caaacaagaa actaaaaccg ttaatattat ttccattctt cccgcaaaag
    15061 ttgccgtaga caactcccaa aataaacgga acgcacaagc cttcggcgcg cttattggcg
    15121 cagtcgctgg cggtgttatc ggccacaacg tcgggtctgg cagcaattcc ggaacgacgg
    15181 caggtgcagt tggcggcgga gctgtaggcg cggcagcggg ttctatggtg aatgataaaa
    15241 ccttagtgga aggtgtttct ttaacctata aggaaggcac caaagtgtat acctctaccc
    15301 aggtgggtaa agagtgccag tttacgacag gtttagccgt tgttattacc acgacgtata
    15361 acgaaacgcg tattcagcca aataccaaat gtcctgaaaa gagctaataa tcaggaggag
    15421 tcatgaagaa agtttttctt tgcgccatct tagcctcctt aagctatccg gctatcgcct
    15481 catcattgca ggatcaactc tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag
    15541 agcaaaggca gcatgacgaa tgggtcgcgg agcgcaacag ggaaatccag caagagaagc
    15601 aacgtcgcgc aaatgcccag gccgccgcta acaaaagagc ggcaacggca gcggcaaata
    15661 agaaagctcg tcaggataaa ctggacgccg aagcctctgc ggacaaaaaa cgcgatcaaa
    15721 gttatgaaga tgagctacgc agcttagaga ttcagaaaca aaaactggcg ctggcgaaag
    15781 aagaagcccg cgttaagcga gaaaacgaat ttatcgatca ggaactgaag cacaaagctg
    15841 cgcaaaccga tgtggtgcaa tctgaagctg acgccaacag aaatatgact gaaggcggtc
    15901 gcgatctgat gaaaagcgtg ggcaaagcag aagagaacaa atcggacagc tggtttaatt
    15961 aatcgatgtt agtaacttca atcctataat tcttgaagat aaaaaaccct ctgtagtaac
    16021 agagggtttt gttcattcat agtgcagggt caaatcattc ccactcgatg atcaatgctg
    16081 tcttaaaaca ctataaaatc aataaaatag cattatatct gcatgtcgat accatacaaa
    16141 ataccatggt aagaaaactt ggagactgta ctgatcgtgt cgcccgacgc aaaacgcttt
    16201 tagactgaca acttgcacac cacaaaggat attcgtccaa aagcagttat tcaaagaagt
    16261 caacgccctg ggttggatct tccgcttgcc tccaccagac ttacatcatc acgaagtaat
    16321 gtcaccatcg cttcatgatg tgctgttatc tgctgatcat cccagtttgt atttcgggtg
    16381 atttccgcca ttctcttgag cttgggactt tgaagttcaa ttgtatcctt gaagttttcg
    16441 gcctttgctc tggggaggct attgctgaat tttgagttcg cgctgacgct tacgagcgcg
    16501 aggttgccaa gcaggttaag gctagaacct gaaacaaccg cgcccgactg ttctttgtca
    16561 ggatgctgtg gatagaagtg ctcaatggaa gttctgaact gaaatttgaa atctcgtttt
    16621 gaggagtcac tcaacagaag atagtcgaga tacgaaaaga cgatacggct gataccaaac
    16681 ccttgtggtt gctgatcttt ctcaaaggag aatgtctctt taaccttgct gcgggcatat
    16741 cccttcaaca aatcggcaag atccgcatgt ttcacgtctc gaggggcttt aacagagagc
    16801 cagcgaagaa ctttggtaat ccagtgcatc gttcgcggag aagtgtaggt aatccgaagc
    16861 atggactgaa gcagcaatac atctcgggta tgtgggtcag cactcccgtc ctcctctaag
    16921 cttccattgc tggccgagaa aacatggatg tacgtcgggg ttggcttctt gtccccgcct
    16981 ccttttttca gtcgctggag agaccaatcg ccatcgtcac cgatgtttgc cgtgaattga
    17041 cgcttcaaga tgaagccatc gaagaggttg cggcacttga gaagcataaa tgcaaaacta
    17101 cgcacccaat cggctttagc atcgggtaca ttatttacgg cgtcgtcaaa tgacttgatg
    17161 aggcgcttgt cgtcaagcag gccttcgtct tcgacctcat cgcctttcat aattcttaga
    17221 acgtgtagca gaaaggccgg aaactcaata gttgaacgaa accgctcgtt gccctcgtct
    17281 tcagtggaat ttgactcgag ctctttagcg tattttgaca acgcctcatc caaggacaac
    17341 gatacccctt cggaggattg ccttgatgaa ttgatgccgg attgcggacg actttccatt
    17401 agcgaagaga agctggtcac ctccagccag gaccattcat caccaaatac tttgtttcgc
    17461 aaacttgtgt caccacgggt cagagacatc tggacataag aatccatgtc agcacaggaa
    17521 tcccaaaccc acgcaaagca ttcgcgttca tattgattgg gcagtttgct catcaggcga
    17581 gccttaacaa tatcaacctg cttgagttgc tgcccacgag tgttcatgat ctcgaagtag
    17641 cgattgaggt cggtattggg tggcagcgag gcacggacga cagtcacctt cgttagcaag
    17701 aaatcggaga atttttcgcg tgagcgatta aggttttcat tttgtttgaa gaactgattg
    17761 atgatgctgt agccttcatg aatgccagca tctgcattac tcgtggagcc ctgcggtcga
    17821 acgtgctcct tagccgcctc ctgtgctact cgcagcaggg cctcagttgc ccgtgcgcgc
    17881 gactcatatt gcaagtggcc tgcatggcca accgacggct tctcaccctc accgtcttgc
    17941 tccaagaagg taagaagcag atagagggtt gtaagtcgtt gttgtccatc tatgacctcg
    18001 aactcatcct ctctaccccg cttgattaca acaaggttcc caagaaaata gttgtcctgg
    18061 ccccctacca ccgagtcttg aatatcgcta ataagttgct caatctgttc ggcccgccaa
    18121 gcatagttcc gttgataaat aggaatcaga taattgtcag tgaataactt gcctactgtt
    18181 aacagttcag gtttcaactc cttcttgctc attgcagccc cttgataaaa gcaacgagtt
    18241 ccttttcgtg gttatcgctg tatggcttac ttgttattgg aagtttgcgg acaacacttc
    18301 cagtcatcgc attacgtagc aaaacaaaag gcgatttatt tgcatcgttt ttacctcgcg
    18361 ctcggttgtc agcagataca aattgcacac gaagtaactc gacccgcaag gcatatgccc
    18421 atgcgaataa cctatttcgc acttcatcta catcttcatc gccaaacttg tttgtatagc
    18481 aaagcagcgc ggcaatgaac aattcgtaca cgtagcggta gcgactttga gatgggccaa
    18541 agtttttgaa acctctgtca ataacctctt gttccagtat cttcaattcg tcaagcatga
    18601 atgtcaccat ctcgaaaaat gagcgtcctg cgattatcgg ggcatcaagc tgaaagcggc
    18661 tgcgcccggc atttcgtgca tcatgtgtag aggataccga ccatgcactt agaagtggca
    18721 tagcggcttg tgctgcaagg tgatatcgca gatttggcga aaatgacctg tgggatcgtg
    18781 atgaaatgcc cttgaacatg ccgatgtccc gaatagtaaa ttcgagcgag ctttcgcctc
    18841 gtgaccactt ggatatccgg tataaaaaag tagaaaaaag cctgtccaga tcctcatcgt
    18901 ttacggcttc ccaagcctca acgacagcca ctttcatagc agcggactca tcgtgcattt
    18961 cgcgcaggtg gtgtgctttg agcaagtcgt gaggggcaag cggtttgccg cgatagttct
    19021 gggaatcaaa gacacgaaat gcctcatcaa tatcgtccgt cacaatacgc accaactgac
    19081 atttgtgaca gataaaatcc aaaaactctt ttttatcttc caactgcgac aggcgccttt
    19141 gaagctctat ccaaactagg gagacaggag tttcgctgtt accagacatc gaaatttgat
    19201 gatttatcgg atctaaggcc gcaagaatca ttcgcaaagt cagcaggcgc tgctgcccgt
    19261 cgactacatt gagatactca ccatcatcgt gaagaatgat tgcacccaaa acatagggaa
    19321 tatcttttcg ttcggtatct cgaagagctt cgctgatatc atccaccagt tgtagagcag
    19381 tcgatacatc ccaactgtat gggcgttgat agttcggtat ccgaaggcct tgcttgagta
    19441 gttcatccac ggtgactgca ctaacaccgt tctctccgtt aatcgccatt cctatgtccc
    19501 ttcattgacg cttttctatt ttctcagcgt attttccgca aactttattc acctatatgg
    19561 atacttcgat tccgagtggc ggctgtcttg ttcacaattt ctatcacgag gtgctcctct
    19621 ggcgcatatt taacgatctg gggcatgggt cgagagagcg gttctgatca aagcgagcat
    19681 cgatggcatc agcacagcgc tgcaatgcac ccggttgtcg ataccatggc atttgctgat
    19741 acggtcacat ggtatttgct atatcttcga ttcagcgtcg gccttaaacc ataaaaaatg
    19801 ccattgataa tgcagtcata tcatcaatgg cactcactaa atcaaataaa acaataggct
    19861 aaataatagc ctcactccca ctcaatggta gctggcggct tgccgctgat gtcatacacc
    19921 acgcgggaaa taccgttcac ttcattgata atgcggttgg aaacgcgacc gaggaaatcg
    19981 tacggcagat gcgcccagtg tgcggtcata aagtcgatgg tttcgacagc acgcagagag
    20041 acaacccagt catacttacg accatcgccc attacgccaa cggaacgtac cggcaggaac
    20101 acagtgaacg cctggctgac tttgtcgtac aggtccgctt tacgcagttc ttcaatgaag
    20161 atggcgtcag cacggcgcag caggtcacag tactctttct tcacttcacc cagcacacga
    20221 acgccaaggc ctggtcccgg gaatgggtga cggtacaaca tgtcgtacgg caggcccagc
    20281 tccagaccaa tcttacgcac ttcgtctttg aacagctctt tcagcggttc aaccaggccc
    20341 atcttcatct ctttcggcag gccgcccacg ttgtggtgag atttgatgac gtgtgcttta
    20401 ccggttgcag acgccgcaga ttcgataacg tcagggtaga tggtgccctg cgccagccac
    20461 ttcacgtctt ccagtttcag cgcttcttca tcgaatactt caacgaaaac gcgaccgatg
    20521 attttacgtt ttgcttccgg atcgttttcg ccagccagcg ctgacaggaa gcgatcttct
    20581 gccggaacgt gaacaatgtt cagaccaaag tgatcgccaa acatatccag aacctgctct
    20641 gcttcgttga gacgcagcag gccgttgtcg acgaatacgc aagtcaggtt tttaccgata
    20701 gcgcggtgca gtagcattgc ggttacggag gaatccacgc caccagagag gccgaggatg
    20761 actttatcgt cgcctacctg ctcacggatg cgggctacag catcgtcgat aattttcgct
    20821 ggcgtccaca gggcttcaca ctggcagata tcacgcacaa aacgctccag catgcgcata
    20881 ccctggcggg tgtgagtcac ttccgggtgg aactgtacgc catagaagcg tttttcttcg
    20941 ttagccataa tggcaaacgg gcagctt
//
LOCUS       DACAPB010000069        20832 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.069, whole genome shotgun sequence.
ACCESSION   DACAPB010000069 DACAPB010000000
VERSION     DACAPB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.069"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            272..1132
                     /locus_tag="IT904_002290"
     CDS             272..1132
                     /locus_tag="IT904_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAP3192063.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            1271..1906
                     /gene="argO"
                     /locus_tag="IT904_002291"
     CDS             1271..1906
                     /gene="argO"
                     /locus_tag="IT904_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAP3192064.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            1999..2739
                     /locus_tag="IT904_002292"
     CDS             1999..2739
                     /locus_tag="IT904_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAP3192065.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            2907..3461
                     /locus_tag="IT904_002293"
     CDS             2907..3461
                     /locus_tag="IT904_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3192066.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAPPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQE"
     gene            3492..3803
                     /locus_tag="IT904_002294"
     CDS             3492..3803
                     /locus_tag="IT904_002294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192067.1"
                     /translation="MRFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGH
                     FLTVIPHFAWRLVNPATLKYFDAPHKPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTS
                     H"
     gene            complement(3800..5278)
                     /gene="scpC"
                     /locus_tag="IT904_002295"
     CDS             complement(3800..5278)
                     /gene="scpC"
                     /locus_tag="IT904_002295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAP3192068.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5302..6087)
                     /gene="scpB"
                     /locus_tag="IT904_002296"
     CDS             complement(5302..6087)
                     /gene="scpB"
                     /locus_tag="IT904_002296"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAP3192069.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(6098..7093)
                     /gene="meaB"
                     /locus_tag="IT904_002297"
     CDS             complement(6098..7093)
                     /gene="meaB"
                     /locus_tag="IT904_002297"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAP3192070.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIHPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(7086..9230)
                     /gene="scpA"
                     /locus_tag="IT904_002298"
     CDS             complement(7086..9230)
                     /gene="scpA"
                     /locus_tag="IT904_002298"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAP3192071.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            complement(9434..10327)
                     /locus_tag="IT904_002299"
     CDS             complement(9434..10327)
                     /locus_tag="IT904_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAP3192072.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10469..10699
                     /locus_tag="IT904_002300"
     CDS             10469..10699
                     /locus_tag="IT904_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192073.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLENGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            10755..11414
                     /gene="rpiA"
                     /locus_tag="IT904_002301"
     CDS             10755..11414
                     /gene="rpiA"
                     /locus_tag="IT904_002301"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP3192074.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            11670..12902
                     /locus_tag="IT904_002302"
     CDS             11670..12902
                     /locus_tag="IT904_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP3192075.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            13131..13190
                     /gene="ibsC"
                     /locus_tag="IT904_002303"
     CDS             13131..13190
                     /gene="ibsC"
                     /locus_tag="IT904_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAP3192076.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(13291..13893)
                     /locus_tag="IT904_002304"
     CDS             complement(13291..13893)
                     /locus_tag="IT904_002304"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP3192077.1"
                     /translation="MTQLPELPLILSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(13914..14097)
                     /gene="ssrS"
                     /locus_tag="IT904_002305"
     ncRNA           complement(13914..14097)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IT904_002305"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(14139..14468)
                     /gene="zapA"
                     /locus_tag="IT904_002306"
     CDS             complement(14139..14468)
                     /gene="zapA"
                     /locus_tag="IT904_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP3192078.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            14636..15214
                     /gene="ygfB"
                     /locus_tag="IT904_002307"
     CDS             14636..15214
                     /gene="ygfB"
                     /locus_tag="IT904_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAP3192079.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            15240..16565
                     /gene="pepP"
                     /locus_tag="IT904_002308"
     CDS             15240..16565
                     /gene="pepP"
                     /locus_tag="IT904_002308"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP3192080.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            16562..17740
                     /gene="ubiH"
                     /locus_tag="IT904_002309"
                     /gene_synonym="visB"
     CDS             16562..17740
                     /gene="ubiH"
                     /locus_tag="IT904_002309"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAP3192081.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATTCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            17763..18965
                     /gene="ubiI"
                     /locus_tag="IT904_002310"
                     /gene_synonym="visC"
     CDS             17763..18965
                     /gene="ubiI"
                     /locus_tag="IT904_002310"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAP3192082.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFASHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(19039..19122)
                     /locus_tag="IT904_002311"
     CDS             complement(19039..19122)
                     /locus_tag="IT904_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192083.1"
                     /translation="MSKNTKSKNNGIRKYNAKMEVKLVYFK"
     gene            19413..20507
                     /gene="gcvT"
                     /locus_tag="IT904_002312"
     CDS             19413..20507
                     /gene="gcvT"
                     /locus_tag="IT904_002312"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAP3192084.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            20531..>20832
                     /gene="gcvH"
                     /locus_tag="IT904_002313"
     CDS             20531..>20832
                     /gene="gcvH"
                     /locus_tag="IT904_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAP3192085.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGG"
BASE COUNT         4872 a         5197 c         5605 g         5158 t
ORIGIN      
        1 ggcctacacg tatcctgcaa tatattgaat ttgcaggttt ttgtaggccg gataaggcgt
       61 tcacgccgca tccggcatga caaaacgcac attgatatct gtctgagccc cgctctgcgg
      121 ggattttttc gccaaaagca aggtgattca gatgagaaat ctgtgatcta tttggcaaaa
      181 ttatgcttta ttgtttaccc ttgtcagact gcccgtcata aggcggcgga gtgtatttct
      241 ccattttgag tcagttgaaa aggaatattg aatggaagat ttgaatgttg tcgatagcat
      301 aaacggcgcg ggaagctggc tggtagctaa ccaggcgctg ctgctaagtt atgcagtaaa
      361 catcgtggcg gcactcgcga tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa
      421 cgcggtgaat cgcctgatga tctcccgcaa aatcgatgcc actgttgctg attttctttc
      481 tgcattagtc cgttacggta ttatcgcctt tacgctaatc gcagccctgg gacgcgtggg
      541 tgtacaaaca gcgtcagtca ttgctgtact cggtgccgca ggcttagctg ttggtctggc
      601 tttgcagggg tcactttcta acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt
      661 ccgtgccgga gaatatgttg acctcggcgg cgtagccggt actgtgctga gtgtgcagat
      721 tttctccacc accatgcgta ctgcagacgg taaaattatc gttattccga acggtaaaat
      781 tattgccgga aatattatta acttctcccg cgagccagtt cgccgtaacg aatttattat
      841 tggcgtggcg tatgattccg atatcgatca ggttaagcag atcctgacca atattatcca
      901 gtctgaagat cgcattttga aagatcgcga aatgactgtg cgcctgaacg aacttggtgc
      961 atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta
     1021 ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc gccggtatca gcttcccgta
     1081 cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct
     1141 gaggccagat aatactctct ggcctctctc ttattagtta ttctgattgc caattaatat
     1201 tatcaatttc cgctaataac aatcccgcga tatagtctct gcatcagata cttaattcgg
     1261 aatatccaac gtgttttctt attactttca aggtcttgct cttggggcag ctatgattct
     1321 gccgctcggc ccacaaaatg cttttgtgat gaatcagggc attcgtcgtc agtaccacat
     1381 tatgattgcc ttactttgcg ctatcagcga tttggtcctg atttgcgcgg ggatttttgg
     1441 tggtagcgcg ttattgatgc agtcgccgtg gttgctggcg ctggtcacct ggggcggcgt
     1501 agtcttcttg ctgtggtatg gttttggcgc ttttaaaaca gcgatgagta gtaatattga
     1561 gttagctagt gccgaagtcc tgaagcaagg cagatggaaa attatcgcca ccatgttggc
     1621 agtgacctgg ctgaatccgc atgtttacct ggatactttt gttgtactgg gcagccttgg
     1681 cgggcagctt gacgtcgaac caaaacgttg gtttgcgctc gggacaatta gcgcctcttt
     1741 cctgtggttc tttggtctgg ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc
     1801 aaaatcacag cgcattatca atctggtagt gggatgtgtc atgtggttta tagccttgca
     1861 gctggcgaga gacggtattg ctcatgcaca agccttgttc agttaggcgc tatctgatgg
     1921 aaaaataaaa cagaggcgct aagcttgcct ccagaggtcc tgaattttgg gcaagtcaat
     1981 taacacggag agactaacgt gaagttcaaa gttatcgccc tggcggcatt aatgggtatt
     2041 agcgggatgg cagcgcaggc taacgaattg ccggatggac cgcatattgt cacctccggt
     2101 acggcaagtg tggatgcggt gccagacatt gccactcttg cgattgaagt taacgtggcc
     2161 gcgaaggatg ccgctacagc caagaaacag gcagatgagc gcgtcgcaca atacatttcc
     2221 ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct cagcaaattt acgcacccag
     2281 ccagattatg attatcagga tggtaaaagt atcctgaaag gctaccgcgc tgtgagaacg
     2341 gtggaagtca cgctccgtca gttagacaaa ctgaattcct tgctggatgg cgcgctgaag
     2401 gcgggtctta acgaaattcg ttctgtgtcg ctgggcgtgg cgcagccgga tgcctataaa
     2461 gacaaagcgc gtaaggcagc gattgataac gcgattcatc aggcgcagga actggcgaac
     2521 ggctttcatc gtaaactggg gccggtatat agcgtgcgct accatgtttc caactatcag
     2581 cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc cggtgtccgc ccaggaaact
     2641 tacgagcagg ccgctattca gtttgatgat caggtcgatg tggtcttcca gttagaacct
     2701 gtggatcaac aacccgctaa aacacctgca gcacaataat acttgtagcc cgacgtattc
     2761 gtcgggctaa tgcctttacc cgatattgcc tcctttttct gtaattttct gtaaaaccat
     2821 gacgtaatca cctatctttg ctcgcggttt catcaaggat aggctgactg aaacggttaa
     2881 gcggacaagg atgcggggga ggggggatgg acatttttat ttcgaaaaag atgcgcaatt
     2941 tcattttatt agcgcaaacc aataatattg cccgggcggc agaaaaaatc catatgacgg
     3001 cttcgccatt tggtaaaagc attgccgcgc tggaagaaca aattggctat acgctattta
     3061 cccgcaaaga taataatatc agcctcaata aagccggaca ggaactgtat caaaaactgt
     3121 tcccggttta tcaacggctt tctgcgattg ataatgaaat ccataactca gggcgtcgtt
     3181 cacgagaaat tgtgattggc atagataata cttatccgac aattattttc gatcagttga
     3241 ttagtctcgg agataagtat gaaggcgtga ccgccccgcc agttgagttc agcgaaaacg
     3301 gcgttatcga taatcttttt gaccgccagt tagattttat tatctcccca cagcatgtat
     3361 ctgcacgagt tcaggaactg gaaaacctga ctatcagtga actaccaccg ctgcgattag
     3421 gctttctcgt ttctcgccgt tatgaagagc gtcaagagta agagctgctt caggagctgc
     3481 cctggctaca aatgcgcttt cagaaccggg ctaacttcga agcaatgatt gatgcgaata
     3541 tgcgtccttg tgggatcaat cccaccatta tttatcgtcc ctacagtttt atggccaaaa
     3601 tcagcgccgt ggagcgcgga cattttctga cggtgatccc gcattttgcc tggcgactgg
     3661 tgaatccggc aacgctgaaa tattttgatg ctccgcacaa gccgatgtat atgcaggaat
     3721 atctttattc aatcagaaat catcgttata ccgccacgat gcttcagcat attgctgaag
     3781 atcgtgacgg gacgagtcat taacccagca tcgagccggt tgcaattaaa ttacggtgta
     3841 agtcgaagac gtggctaaga tcgtggtgaa tatgtccgcc aggcgcattt tccagatagc
     3901 gatgcagata atcccgatac ataggatgtg cacaattatc aatgatagtg cgggcgcgtt
     3961 gaagcgggga aagaccgcgc agatcggcga tcccttgttc ggtgatgatc actttgacgc
     4021 tgtgttcgct gtgatcaaca tggctgcaca ttggcacgac ggttgagatc ttgccttctt
     4081 tagcaatcga cggagccata aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc
     4141 cgccgatgcc gttcatcaga tcgaccccgg ctacgtgcgt tgaatttgca tgcccgtaaa
     4201 tatcaaactc caggccgacg ttcagagcga tgacgcccag acgacggatg atttccgggt
     4261 tattggaaat ctcctgcgga cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt
     4321 aaatcttgcg cagggaatcg gccgagattg tcaggctgga ggcgctggcc ccgctgattt
     4381 tgccggtttc cagtaaatgc accaccgatt cctgtagcac ttccgaatac atcataaacg
     4441 gaggaatttc cgggttttcc cccagacgcg ccattaccgc attattgata ttgcccacgc
     4501 cactttgcag cggcagaaat tccggcggaa tacgcccatg cgccatttcc tgcaacaaga
     4561 acgtgaccac gttatcggca atctgctggc acatgggatt ttgcttatcc agcatattac
     4621 cggcgtcggg caagttggtt tccacgacgg cgacaatctt tttcggatcg atttgcacat
     4681 agcgggtacc gacgcgatcc attgcatgga agatcgacac gctattgcgc cgcggtggcg
     4741 cgccaggaat cacaatatcc gccagttctg caacgcgcgg atcgtgatag tgattgagtt
     4801 caatgatcac tttcttcgcc cgcagcagcc aggtcggcgc attaccgatc ccgctggtta
     4861 accagactcg accatccggt gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc
     4921 cgaagaaacc gtaattgacc atttgcgcca cttcgctcaa atgcaggtca acgaaactca
     4981 ccgcgccctg attgatcttt ttacgtaaac cggacgatgt ttgatatggc gcacgccagg
     5041 aaacagcatc ggcgtcagaa agtacatcgt cagcggcggc gctgattgac gcacccgtca
     5101 gaaggcgaat ttgatacggc tttttggcct catgctgttc gttagctctg cgggcaatcg
     5161 cggtgggtag ggctttcggc gaacccgccg gggtaaagcc gctaaatgcc accatgtcgt
     5221 tatgctggat aatttctgcc gcttcatcgg cggtcatcct tgtccactga gtttccattt
     5281 ttactccttc gttcgcaggg attaatgacc aacgaaatta ggtttacgtt tttcgaggaa
     5341 agcgttcatc ccttcctggt aatcttcgct gtcatacacc gcgcggcgca tcccctgaat
     5401 acgttcaaat tcatcggagt tcatggtgtg tgcttcgccc agtacacgca gctcttcttt
     5461 gataacggca atggctaacg gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa
     5521 atcttccagt tcttccactt ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg
     5581 ggcggtgatt ggcgaagcgg taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg
     5641 ggtcaggttg tgaatgccga ccaggttata cgggacgccg aggtttacag gcgtcattga
     5701 gaaggttgag gtactggcgg cgatgatcag atcggaactc atgatcattt caaatgcgcc
     5761 accccaaaca ctaccttcca ccatcgaaat gatcggtttc gggaattttt ggatcatgcg
     5821 ggtgatttga cgcaatggat catcatagga gagcggatcg cgaccgccag acggcagttc
     5881 gtgaatatcg tgacctgctg agaagacttt ggatccactc ggcgcgcgca aaatgataca
     5941 gcgaatttcc ggccggttga gatcgcttaa cgcctgcata agatcatcaa taaagacttt
     6001 acttaaggca ttaagttttc ggccatagtt aaactcaatg accgccactt tgttgatagt
     6061 ggcaacgttc acatactgat aagacataaa aattccttta atcaaaatat tgcgtctgga
     6121 taaattcact gagctgccgc aggccggtgc gcggtgagag cgtattgttt ttgaccgcta
     6181 aaagcgtctg gcggtaatag cgatcgaaat cttcattcgc gaacaggtga ttcagtactt
     6241 cttcttcggt ctgcttacgc agccattcca ccgattgttg ttgccgcact tgttgtaaac
     6301 gaccactggc agttagcgcg gttttgaagt cgatgatggc gtgccagatc tcatcgattc
     6361 cacgtttttc cagtgcgcta caagtcagaa cccgtggctg ccattcgtcg tatttacgtc
     6421 gcagaatatg cagggcactc tcgtacatat gccgggcaat ggcgacattg gtatggttat
     6481 cgccatcgtc tttgttgata acgatcagat cagccacttc catcagccct tttttaatgc
     6541 cctgcagatc atcgccgcca ccggcaattt gcaacgagat aaaacagtcc accatgcggg
     6601 cgacttccgt ttccgactgc ccgacgccaa ccgtttcgac aatcactacg tcataacccg
     6661 ccgcttcgca taacagcatt aattcccgcg ctcgctgact ggcaccgccc agatgaccgg
     6721 aggatggtac cgggtgaata aacgccgtct cggcacgcgc caggtcattc atgcgggttt
     6781 tatccccgag aatgctaccg ccagtgaccg gactgctggg atcgaccgca ataaccgcaa
     6841 cctttaatcc atcacgaatc aacaacatgc caaaggcttc aagaaaggta cttttccccg
     6901 cgccgggtgt gccggtaacg cccagtcgca gggcgttacc gcagtacggc ataatggcat
     6961 caagcagctg cgtacttagt gcctgatgac gcgggtgacg gctttccacc agcgtcatgg
     7021 cctgggcgag tgtggcacgc tcaccctgac gtaagcggcg aatactttct gccagcgtgg
     7081 cttcattaat catgatgctg gcttatcaga ttcagtacgc cgcgcacgct gtcgagcata
     7141 ggtgtacctg gaccataaat cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc
     7201 ggtggaatga cgccaccggc gactacgcag atatcttcgc gtccccattt tttcagcgct
     7261 tcgaccagtt ccgggatcag cgttttatga ccggcagcca gtgaggatgc gcccactacg
     7321 tgaacgtcat tttctactgc caggcgggcg atctcttcag gtgtagagaa catcgggctt
     7381 aaatctacgt cgaaaccgag atcggaatag gcgctggcga tcactttcgc gccgcgatcg
     7441 tgcccatcct ggcccatttt ggcgatcaga atgcgcgggc gacgaccatt gtcggcaagg
     7501 aactgctccg tttgcgcaac aatggcatcg aactcggagg ctgatttctc agattgatga
     7561 tagctttgcg caatcacgcc ggtaacacac tggcttggca ccagataacg gtcgaaagcg
     7621 gcttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg
     7681 gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt
     7741 acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc
     7801 accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg
     7861 ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc
     7921 tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg
     7981 tcgatctgtt ggataatggc tctggcttgt ttgacgattt gatcggtcag cgactcaatg
     8041 taataggatc cggccagtgg atcgacggtg cggcagagtt ctgattcttc ctggatgatg
     8101 atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca
     8161 aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagtgc ttcaatggtg
     8221 gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag
     8281 tgggtacgca gcgccagtga tttcgggtcc tgtgcgccaa atccactgac cgcttcgctc
     8341 cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat gccgatgccg
     8401 aagaagaacg acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct
     8461 ttgatgtact caatcccatc agcgagcgta aatgctacct gctgcacgca gttggcaccc
     8521 gcttcaccca tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa
     8581 caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggcgggta aatataggtg
     8641 ttgcggcaga ggtactcttt gaggatatcg ttctgaatgg tgccggtcag tttatcaggt
     8701 gtaacacctt gctcttccgc ggcgacgata taaaacgcca gtactggtag cactgcgcca
     8761 ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata
     8821 tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cgcccgccac gcgcgggtta
     8881 tcggagtcgt agcctcggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg
     8941 gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac
     9001 tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc
     9061 ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc
     9121 ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga
     9181 cggctcaatt ccttgttggc aagctgttgc cactcctgca cgttagacat tatttgctcc
     9241 gtaaaacgca atcaaaatga gggtatttgc gcctacggtg agctttttac gtgggtttgt
     9301 cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg
     9361 tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt gctatcaggc
     9421 attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt aacgcatcag
     9481 tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccgtcgtt
     9541 gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc tcgatttgca
     9601 ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag
     9661 aattaacgat atggcagggc acgctgcctg gaggcagatc gaagttttgc tgcaaaaagg
     9721 cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcagt aatgccgaac
     9781 gcgttacgcc gttagggaaa tatttttcgg caaagggttt tgagctgacg aacagatagt
     9841 cgagcgcacc gagtttatcg acaagacaac tcggcagcgc ctgatgttga atactcaccg
     9901 cgccgaccac ttcgccgcgg cgcagacgtt cctgagtgcg ggtttcatct tctacctgca
     9961 agttgaggcg gataggcgaa tcagccaaca caggagccag tgccggaagc aaccacgtcg
    10021 ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat
    10081 cgcccagcca ctcttcttcc agcaactcca cctggcgcag cagtgccagc agtttttgcc
    10141 cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt
    10201 ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc
    10261 gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc
    10321 gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cacaattttg
    10381 tgtcaggttg catataccga agccgtcggc gggtaacatt tgtattcggg gttaaacgcg
    10441 cagcttaaaa gatcagggat ttgcgctaat gcatttcgct cagcgagttc gcgccctggt
    10501 tgtgttgaat ggcgtggcgt tattgcctca atttgcctgt aaacaggggc ttgagaacgg
    10561 tgaactggtg cgcctgtttg caccgtggag cggcataccc agaccattgt atgctttatt
    10621 tgcggggcga aaggggatgc ccgccattgc gcgatatttt atggatgagt taaccacccg
    10681 gcttgccaac ggggtctgaa tcgctttttt tgtatataat gcgtgtgaaa tttcatacca
    10741 caggcgaaac gatcatgacg caggatgaat tgaaaaaagc agtaggatgg gcggcacttc
    10801 agtatgttca gcccggcacc attgttggtg taggtacagg ttccaccgcc gcacacttta
    10861 ttgacgcgct cggtacaatg aaaggccaga ttgaaggggc cgtttccagt tcagatgctt
    10921 ccactgaaaa actgaaaagc ctcggcattc acgtttttga tctcaacgaa gtcgacagcc
    10981 ttggcatcta cgttgatggc gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg
    11041 gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt tgcagaaaaa tttatctgta
    11101 ttgcagacgc ttccaagcag gttgatattc tgggtaaatt cccgctgcca gtagaagtta
    11161 tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa actgggcggt cgtccggaat
    11221 accgtcaggg cgtggtgacc gataatggca acgtgatcct cgacgtccac ggcatggaaa
    11281 tccttgaccc gatagcgatg gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg
    11341 gcttgtttgc taaccgtggc gcggatgttg cgctgattgg cacacctgac ggtgtcaaaa
    11401 ccattgtgaa atgatctgac gggggaacct cccccgttaa aaaaattctc ttcattaaat
    11461 ttggtgacat gtgtcacgct tttaccaggc aattgtcgat tgctctaaat aaatcctcta
    11521 aaccagcata ttcatccaag aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa
    11581 acgttcatat tgccgcaata ttattttttg atatgttgaa aggcggatgc aaatccgcac
    11641 acaacatttc aaaagacagg attgggtaaa tggcaaaggt atcgctggag aaagacaaga
    11701 ttaagtttct gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagcgg
    11761 gttacaccaa catcgaattt cacaaaggcg cgctggatga tgaacaatta aaagaatcca
    11821 tccgcgatgc ccacttcatc ggcctgcgat cccgtaccca tctgactgaa gacgtgatca
    11881 acgccgcaga aaaactggtc gctattggct gtttctgtat cggaacaaac caggttgatc
    11941 tggatgcggc ggcaaagcgc gggatcccgg tatttaacgc accgttctca aatacgcgct
    12001 ctgttgcgga gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca
    12061 atgctaaagc gcaccgtggc gtgtggaaca aactggcggc gggttctttt gaagcgcgcg
    12121 gcaaaaagct gggtatcatc ggctacggtc atattggtac gcaattgggc attctggctg
    12181 aatcgctggg aatgtatgtt tacttttatg atattgaaaa caaactgccg ctgggcaacg
    12241 ccactcaggt acagcatctt tctgacctgc tgaatatgag cgatgtggtg agtctgcatg
    12301 taccagagaa tccgtccacc aaaaatatga tgggcgcgaa agagatttcg ctaatgaagc
    12361 ccggctcgct gctgattaat gcctcgcgcg gtactgtggt ggatattccg gcgctgtgtg
    12421 acgcgctggc gagcaaacat ctggcggggg cggcaatcga cgtattcccg acggaaccgg
    12481 cgaccaacag cgatccattt acctctccgc tgtgtgaatt tgacaatgtc cttctgacgc
    12541 cacacattgg cggttcgact caggaagcgc aggagaatat cggcctggaa gttgcgggta
    12601 aattgatcaa gtattctgac aatggctcaa cgctctctgc ggtgaacttc ccggaagtct
    12661 cgctgccact gcacggtggg cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc
    12721 taactgcgct caacaaaatt tttgccgagc agggcgtcaa catcgccgcg caatatctgc
    12781 aaacttccgc ccagatgggt tatgtagtta ttgatattga agccgacgaa gacgttgccg
    12841 aaaaagcgct gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt ctgctgtact
    12901 aattcccctt ctctgaaaat caacgggcag gtcactgact tgcccgtttt tttatccctt
    12961 ctccacaccg ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga aaataaatca
    13021 aagtcagatc aatgcgttgt gtttatttaa tggggattta cgatggcagg gcagcatggg
    13081 gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt atgatgcgac
    13141 ttgtcatcat actgattgta ctgttactca taagtttcag cgcttattaa cagtcagtct
    13201 caggggagga gcaatcctcc cttaccctta ctcactaaat taggtcaaag aatcaacgat
    13261 gtcaatcagg gcgatgcagg tgtgtcgccc ttaccactcc cagactttcg acggtgtaac
    13321 caccgcaggc agtgggatat cccactcttc aacggggagt ttttccacca actgacaatc
    13381 atgcgcataa cccaccggtt gcattttata gtgctgccag ttttgtaagg tccgatcata
    13441 aaaacctccg cccattccca ggcgctgacc gtactcatca aaggcgacca ggggggtgat
    13501 caggacgtct aatcgggaaa gggggagcac gtcacgcaca tccaattttg gctcatggat
    13561 cttcaacctg ttcatcacca gttcgctttg cggatggtaa ttcaggaaca gcaaattacc
    13621 ggcactaaag ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc agagttgttc
    13681 tatgagtggc tgggtgtcga gttcgccatc aaaagagagg aatacagcga ccgtatgtgc
    13741 catcaccacc gggggataag tcatcatccg ggtagcggct tgttgaccca tttcctgctg
    13801 ttgttccggc gttaacgcac gacgacgttg ccgaatcatt ttgcggattt cttgtcggga
    13861 taatatcagt gggagttctg gtagttgcgt catggctggt gccagataag aagggaatct
    13921 ccgagatgcc gccgcaggct gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg
    13981 cagttttaag gcttctcgga cgaaccgagc atgctcacca accgcggagc gccacattct
    14041 tgtggtatga aatatcggct caggggactg gcccgcttgc gaacatctca gagaaatttt
    14101 gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt
    14161 ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta tggtctgctg cagcatccga
    14221 atacgctgtt ccatacttgc cgcgtagtca cgagtcttcg ctttttcttg cgctaactca
    14281 tagctgatat tcaatgcggc aatgaagacc aactgttcag tatttgtgac tctagtgcgt
    14341 tctttcagat cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa cgcatccctt
    14401 tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa tttggatatc gacgggttgt
    14461 gcagacatgc caccttcctg ctgattgact gcgctgcctt cgtctttaga cccatggtct
    14521 gcgaaggggc gacactatag ctaccctgat gagaagagac aagccctttt ctggtccacc
    14581 agggcccaaa gtggtagcat atcatgaata ttcctccctt tgacgacgaa tgcttatgtc
    14641 tatacagaac gaaatgcctg gttacaacga aatgaaccag tatctgaacc aacaagggac
    14701 gggtctgacc ccagctgaga tgcatggttt aatcagcggg atgatatgtg gcggtaacga
    14761 tgacagctca tggctaccgc tacttcacga cctgacgaac gaaggcatgg ctttcggtca
    14821 tgagctggca caggcactgc gtaaaatgca ctctgccacc agcgatgccc tgcaggatga
    14881 cggcttcctt tttcagcttt atctgcctga tggcgatgat gtcagcgttt tcgatcgggc
    14941 tgatgcattg gcaggttggg tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa
    15001 gctggataaa gtgaccggcg aaaccggtga agctatcgac gatctgcgta acattgcgca
    15061 actgggttac gacgaagacg aagatcagga agagcttgaa atgtcgcttg aagagatcat
    15121 cgaatacgtt cgtgttgccg cgctgttatg ccacgacacc tttactcatc cgcaaccgac
    15181 cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta
    15241 tgagtgagat atcccggcaa gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc
    15301 aacccggcag cgccgcgctg atttttgctg caccagaagt aacacgtagc gccgacagcg
    15361 aataccccta tcgtcagaac agtgacttct ggtacttcac cggctttaac gaaccggaag
    15421 cggtgctggt gctgattaaa agcgatgaca ctcataacca cagcgttctg tttaaccgcg
    15481 ttcgcgacct gacggcggag atctggtttg gccgtcgctt aggccaggat gccgcgccag
    15541 agaaactggg cgttgaccgc gcactggcat tcagcgaaat caatcagcaa ctttatcaac
    15601 tacttaacgg cctggatgtg gtttaccatg cccagggcga atatgcatat gctgatgaaa
    15661 tcgtgaacag tgcgctggaa aaactgcgta aaggttcgcg gcaaaatctc accgcaccgg
    15721 caacgatgat cgactggcgt cctgttgttc atgaaatgcg cctgttcaaa tcgccagaag
    15781 agattgccgt actccgccgc gcgggagaaa tcaccgccat ggcacataca cgggcgatgg
    15841 aaaaatgccg tccgggaatg ttcgagtacc atctggaagg cgaaattcac cacgaattta
    15901 accgccacgg tgcgcgctat ccgtcctata acaccattgt cggcagcggt gaaaacggct
    15961 gcattctgca ctacaccgaa aacgagtgtg aaatgcgcga cggcgacctg gtgttgattg
    16021 acgcgggttg tgaatacaaa ggttacgctg gcgatattac ccgcaccttc ccggtcaacg
    16081 gcaaattcac tcaggcccag cgtgaaatct acgacattgt gctggagtct cttgaaacca
    16141 gcctgcgcct gtatcgtcca ggaacttcca tgcaggaagt cactggtgaa gtggtgcgca
    16201 tcatggttag cggcctggta aaactcggca tcctgaaagg tgatgttgat gaactgatcg
    16261 ctcagaacgc ccatcgtcct ttctttatgc atggccttag ccactggtta ggactggatg
    16321 tccatgacgt gggtgtttat ggtcaggatc gctcgcgcat tctggaaccg ggcatggtac
    16381 tgaccgtaga gccagggctg tatattgcgc cggatgcaga agtgccagaa caatatcgcg
    16441 gtatcggcat tcgtattgaa gacgacattg tgattaccga aaccggtaac gaaaacctca
    16501 ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt gatggcagct gcgagaaagc
    16561 aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc tggctatttc
    16621 ccggttaagt cacggggcgc tgccggtaca tttgattgaa gcgactgcgc cagagtcaca
    16681 tgctcatccg ggctttgatg gacgagcgat agcgctggcg gcgggtacct gtcagcaact
    16741 ggcgcgcatc ggcgtctggc aatctctagc ggattgcgca actgccatca ccaccgtgca
    16801 tgtcagcgat cgtggtcacg ctggatttgt caccctcgcc gcagaagatt accaactggc
    16861 ggcgctggga caggttgtcg aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg
    16921 taaagcacct ggcgtaacgc tgcattgccc tgatcgcgtg gctaacgttg cccgtactca
    16981 gagtcacgtt gaagtgacgc tggagagtgg cgagacgctg acgggccgcg tgctggtagc
    17041 agctgatggc acccattcag cgttagccac cacgtgcggc gttgactggc agcaggagcc
    17101 ttacgaacaa ctggccgtga ttgccaacgt tgcgacttcc gttgcgcacg aaggccgcgc
    17161 ttttgaacgc ttcacgcaac atggcccgct ggcgatgttg ccgatgtctg acggacgctg
    17221 ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag gtgctgtcgt ggagcgacga
    17281 gaagttttgc cgtgaactcc agtcggcctt tggctggcga cttggaaaaa ttacccacgc
    17341 cggtaaacgc agtgcttatc cgctggcgtt aacccgcgcc gccagagcta ttacccatcg
    17401 taccgtgctg gtgggcaatg cggcgcaaac tctgcacccg attgccgggc aagggtttaa
    17461 cctcggtatg cgagatgtca tgagccttgc ggaaaccctg actcaggcgc atgagcgcgg
    17521 agaagacatc ggtgattacg gcatattgtg ccgctatcag cagcgtcgac agagcgatcg
    17581 cgaagcaacc atcggcgtca cggacagcct ggtacatctt tttgccaacc gttgggcacc
    17641 gctggttgtc gggcgcaaca tcggactgat gacgatggaa ttattcaccc cggcacgcga
    17701 tgtgctggcg cagcgcaccc tcggttgggt ggcgcgttga aggcggtttt aaggaataaa
    17761 caatgcaaag tgttgatgta gccattgttg gtggcggcat ggtggggctg gcggttgcct
    17821 gcggcttaca ggggagcggc ttacgcgttg ccgtactgga gcagcgcgta ccggaacctc
    17881 tggcggcgga tgcaccacca caactgcgcg tttcggctat caatgccgcc agcgaaaaat
    17941 tactcacccg tcttggcgtc tggcaggaca ttctctctcg tagggccagt tgttatcacg
    18001 gtatggaagt gtgggacaaa gacagctttg gtcacatttc gtttgacgat caaagcatgg
    18061 gctatagcca tcttgggcat atcgttgaaa attcagtgat tcactacgcg ctgtggaaca
    18121 aagcgcatca gtcgtcagat atcactctgt tagcccccgc agaattacag caggtcgcct
    18181 ggggagaaaa tgaaaccttc ctgacgctga aagatggcag catgttaacg gcgcgtctgg
    18241 tgattggcgc ggacggcgct aattcctggt tgcgcaacaa agccgatatt ccgctgactt
    18301 tctgggatta tcagcatcac gcgctggtag cgaccattcg cacggaagaa ccgcatgatg
    18361 cggtggcgcg gcaggttttc catggcgaag gcattctggc ctttttaccg cttagcgatc
    18421 cgcatctttg ctcgattgtc tggtcactgt cgccagagga agcgcagcgg atgcagcagg
    18481 caagtgaaga cgaatttaat cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat
    18541 gcaaggttga gagcgcgcgt caggtgttcc cactgacggg gcgttatgcg cgccagtttg
    18601 cctcgcaccg tctggcgctg gtgggcgacg ccgcacatac cattcacccg ctggcggggc
    18661 agggggtaaa tctcggcttt atggatgctg cagagctgat tgccgaactg aaacggttgc
    18721 atcgtcaggg gaaagacatc gggcagtaca tttatctgcg tcgctatgag cgtagccgca
    18781 agcacagtgc ggcgttgatg ctggctggta tgcagggatt ccgcgatctg ttttccggta
    18841 ccaatccggc gaaaaaactg ctgcgtgata ttggtttgaa actggccgac acgcttcctg
    18901 gcgttaagcc gcaacttatc cgccaggcaa tgggattaaa cgatttgcct gaatggctgc
    18961 gttaaaaatt tctcctctgt tgtttatttg atacccatca cacttccatc tcccggtttt
    19021 ttcgccggga gattttcctc atttgaaata aactaatttc acctccattt tcgcattata
    19081 ttttctaatg ccattatttt ttgatttagt gttttttgac atttttttag ctcttaatat
    19141 tgtcttattc aaattgactt tctcatcaca tcatctttgt atagaaactg gtgtattttt
    19201 tggtttttta ttctgtcgcg atttttgcat tttttaacca taagctaatg tgatgatcaa
    19261 ttttacctta tggttaacag tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc
    19321 gttggggacc gggagtggct ccgatgctgg gtttcgtggt gataatttca ccatgaaaaa
    19381 gttgtcagcc ccgcttattc aatgaggaca agatggcaca acagactcct ttgtacgaac
    19441 aacacacgct ttgcggcgct cgcatggtgg atttccacgg ctggatgatg ccgttgcatt
    19501 acggttcgca aatcgacgaa catcatgcgg tacgtaccga tgccggaatg tttgatgtgt
    19561 cacatatgac catcgtcgat ctccgcggca gccgcacccg ggagtttctg cgttatctgc
    19621 tggcgaacga tgtggcgaag ctcaccaaaa gcggcaaagc cctttactcg gggatgttga
    19681 atgcctctgg cggtgtgata gatgatctca tcgtctacta ctttactgaa gatttcttcc
    19741 gcctcgttgt taactccgcc acccgcgaaa aagacctctc ctggattacc caacacgctg
    19801 aacctttcgg catcgaaatt accgttcgtg atgacctttc catgattgcc gtgcaagggc
    19861 cgaatgcgca ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag gcggtggaag
    19921 ggatgaaacc gttctttggc gtgcaggcgg gcgatctgtt tattgccacc actggttata
    19981 ccggtgaagc gggctatgaa attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg
    20041 cgctggtgga agcgggtgtt aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg
    20101 aagcgggcat gaatctttat ggtcaggaga tggacgaaac tatttctcct ttagccgcca
    20161 acatgggctg gactatcgcc tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc
    20221 tggaagtgca gcgtgagcat ggtacagaaa aactggttgg tctggtgatg accgaaaaag
    20281 gcgtgctgcg taatgaactg ccggtacgct ttaccgatgc gcagggcaac cagcatgaag
    20341 gcattatcac cagcggtact ttctccccga cgctgggtta cagcattgcg ctggcgcgcg
    20401 tgccggaagg tattggcgaa acggcgattg tgcaaattcg caaccgtgaa atgccggtta
    20461 aagtgacaaa acctgttttt gtgcgtaacg gcaaagccgt cgcgtgattt acttttttgg
    20521 agattgattg atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct
    20581 gcgtaaagaa gccgacggca cttacaccgt tggtattacc gaacatgctc aggagctgtt
    20641 aggcgatatg gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga
    20701 ctgcgcggtt gccgaatcag taaaagcggc gtcagacatt tatgcgccag taagcggtga
    20761 aatcgtggcg gtaaacgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta
    20821 tgcaggcggc tg
//
LOCUS       DACAPB010000070        20632 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.070, whole genome shotgun sequence.
ACCESSION   DACAPB010000070 DACAPB010000000
VERSION     DACAPB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.070"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..474
                     /locus_tag="IT904_002314"
     CDS             <1..474
                     /locus_tag="IT904_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP3192086.1"
                     /translation="IGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYL
                     ADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTL
                     GGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            844..1668
                     /gene="gadX"
                     /locus_tag="IT904_002315"
     CDS             844..1668
                     /gene="gadX"
                     /locus_tag="IT904_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAP3192087.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLSNRDSAANIVAQGNFYGTDRSAEGIRL"
     gene            2036..2764
                     /gene="gadW"
                     /locus_tag="IT904_002316"
     CDS             2036..2764
                     /gene="gadW"
                     /locus_tag="IT904_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAP3192088.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3127..6240)
                     /locus_tag="IT904_002317"
     CDS             complement(3127..6240)
                     /locus_tag="IT904_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP3192089.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6265..7422)
                     /gene="mdtE"
                     /locus_tag="IT904_002318"
     CDS             complement(6265..7422)
                     /gene="mdtE"
                     /locus_tag="IT904_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAP3192090.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7482..7760
                     /locus_tag="IT904_002319"
     CDS             7482..7760
                     /locus_tag="IT904_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192091.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7761..8288)
                     /gene="gadE"
                     /locus_tag="IT904_002320"
     CDS             complement(7761..8288)
                     /gene="gadE"
                     /locus_tag="IT904_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAP3192092.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9087..9659)
                     /gene="hdeD"
                     /locus_tag="IT904_002321"
     CDS             complement(9087..9659)
                     /gene="hdeD"
                     /locus_tag="IT904_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAP3192093.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9914..10246
                     /gene="hdeA"
                     /locus_tag="IT904_002322"
     CDS             9914..10246
                     /gene="hdeA"
                     /locus_tag="IT904_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAP3192094.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10362..10688
                     /gene="hdeB"
                     /locus_tag="IT904_002323"
     CDS             10362..10688
                     /gene="hdeB"
                     /locus_tag="IT904_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAP3192095.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10752..11399
                     /locus_tag="IT904_002324"
     CDS             10752..11399
                     /locus_tag="IT904_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP3192096.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11441..11971)
                     /locus_tag="IT904_002325"
     CDS             complement(11441..11971)
                     /locus_tag="IT904_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3192097.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12127..12693)
                     /gene="slp"
                     /locus_tag="IT904_002326"
     CDS             complement(12127..12693)
                     /gene="slp"
                     /locus_tag="IT904_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAP3192098.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12941..14164)
                     /locus_tag="IT904_002327"
     CDS             complement(12941..14164)
                     /locus_tag="IT904_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192099.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFLHKEHPTPKLYKDGQYLDKQIEG
                     LFSTLLTINDNLLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKQCYKQVKAFT
                     TLSRYNKIQTWAEMSEYQFEVFQYETLNPKKMSHTPYLKRPLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(14774..15199)
                     /gene="arsC"
                     /locus_tag="IT904_002328"
     CDS             complement(14774..15199)
                     /gene="arsC"
                     /locus_tag="IT904_002328"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAP3192100.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15212..16501)
                     /gene="arsB"
                     /locus_tag="IT904_002329"
     CDS             complement(15212..16501)
                     /gene="arsB"
                     /locus_tag="IT904_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAP3192101.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(16555..16908)
                     /gene="arsR"
                     /locus_tag="IT904_002330"
     CDS             complement(16555..16908)
                     /gene="arsR"
                     /locus_tag="IT904_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAP3192102.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            17648..17731
                     /locus_tag="IT904_002331"
     CDS             17648..17731
                     /locus_tag="IT904_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAP3192103.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(17785..19137)
                     /gene="gorA"
                     /locus_tag="IT904_002332"
     CDS             complement(17785..19137)
                     /gene="gorA"
                     /locus_tag="IT904_002332"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP3192104.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKVVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(19209..20051)
                     /locus_tag="IT904_002333"
     CDS             complement(19209..20051)
                     /locus_tag="IT904_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAP3192105.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARLLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGIYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            20254..>20632
                     /locus_tag="IT904_002334"
     CDS             20254..>20632
                     /locus_tag="IT904_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAP3192106.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQ"
BASE COUNT         5512 a         4712 c         4796 g         5612 t
ORIGIN      
        1 attggtactt ttgccatcaa cttctcccgc ccggcgggtc aggtaattgc acagtactat
       61 gaattcctgc gcctcggtcg tgaaggctat accaaagtac agaacgcctc ttaccaggtt
      121 gccgcttatc tggcggatga aatcgccaaa ctggggccgt atgagttcat ctgtacgggt
      181 cgcccggacg aaggcatccc ggcggtttgc ttcaaactga aagatggtga agatccggga
      241 tacaccctgt acgacctctc tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc
      301 actctcggcg gtgaagccac cgacatcgtg gtgatgcgca ttatgtgtcg tcgcggcttc
      361 gaaatggact ttgctgaact gttgctggaa gactacaaag cctccctgaa atatctcagc
      421 gatcacccga aactgcaggg tattgcccag cagaacagct ttaaacacac ctgataacat
      481 aacgttgtaa aaaccgaatg cccagccttt aaaaaaacag ctgggcattc ggttgcttat
      541 taatcgcaat aatatattgg ctgtctattc atcgtgttga taagatatat gttatatgaa
      601 tgtttattaa atgtagcgat tacctttaat gtcacgaaaa gggattattt gcttactatt
      661 aatttccctg cgttcttaaa cacgtttttg tattataaat ccagtcatcc tgcccgtaga
      721 acatcacaca ttatcatcct gttctcccgc ttcgtttaaa tttatttatc aatcaatttg
      781 acttaagagg gcggcgtgct acattaataa acagtaatat gtttatgtaa tattaagtca
      841 actatgcaat cactacatgg gaattgtcta attgcgtatg caagacataa atatattctc
      901 accatggtta atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca
      961 agccaaattc gagtagataa gtgtgttgaa aattttgtat tcgtgtcaag ggacacgctt
     1021 tcattatttc tcccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaagtt
     1081 tcttcattac tcgttcatca ctgtagtaga gatattcctg tttttcagga agttgcgcaa
     1141 ctatcgcaga ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt
     1201 gcgttgttat ctgtttttct tgaggatgag cactttatac cgctgcttct gaacgtttta
     1261 caaccgaaca tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg
     1321 acactagccc gaatcgccag cgagctgttg atgagtccaa gtctgttaaa gaaaaaattg
     1381 cgcgaagaag agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg
     1441 caacttattg ttatacatgg tttttcaatt aagcgagttg cagtatcctg tggatatcac
     1501 agcgtgtcgt atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat
     1561 caggagcgat cggcgcagag attgtcgaac cgtgactcgg cggcaaatat tgttgcgcaa
     1621 gggaattttt acggcactga ccgttctgcg gaaggaataa gattatagag ttttactcag
     1681 acataaaaaa aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa
     1741 taaaccgtta taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata
     1801 taacttttac tggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg
     1861 aaaatatatt cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt
     1921 ggataaattt tggtcctacc aaatctggca gtttttgcgc taagaaacag tctggcatca
     1981 tttcattagt atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac
     2041 tcatgtctgc tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcaaaa
     2101 aatatcgctg cataacgaga gtattctgct gctggagaaa aatttggcag acgattttgc
     2161 gttttgttca ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt
     2221 acaaaatatt cgtcagctac cacgcaattt agggttacac agcaaagacc gtttgttaat
     2281 taaccagtca ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg
     2341 ggtaaattca ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca
     2401 taagaaagaa ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga
     2461 acgccttatt agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat
     2521 gtataccagc gagagtctaa tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa
     2581 aatattactc gcctccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc
     2641 tctgcatact attgcggaaa aatgtggcta tagcagtaca tcgtacttta taaacacatt
     2701 tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc
     2761 ctgacatatt tcgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct
     2821 tccctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat
     2881 cggtattttc aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac
     2941 gattacggga atgtgggtca gtattgtgtt gttcaaattg atgacgaatg gctggagtgg
     3001 tttctacttt cagtgctgtg tactaagtct ggtattcctg acggttagct ggttgttgag
     3061 tggtgaatgg ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt
     3121 tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg
     3181 aacgaagtaa attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac
     3241 cgcgttttgc gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat
     3301 aaaggccaga gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc
     3361 ctctatcggc gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat
     3421 ggcgtttttg gcggaaagcc cgatggtggt cagcaaacca acctggaagt agacgtcatt
     3481 acttaagccg cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa
     3541 catcaccgag aacggaattg accagctctc atagagtgcg gcgagggcga ggaacaccac
     3601 gaccagtgaa atcgcataca gcgcaggagc ctgatttgag gataacgctt cctgatacga
     3661 tagtccggtc catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa
     3721 tttcatggcg tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg
     3781 gatgccgttg tagcgttcca gtcgcggtga accataggtc cattcggtag acgagtaggc
     3841 agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg
     3901 caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag
     3961 gaagtcgttc acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag
     4021 cgccacgccc atcgcttcag ctttcgcagc gttgacgttc actttgaaca tcggcgtatc
     4081 ttccaggccg ttcgggcgta ccccggtgac ctgattcggt gattgcgctg ccagtgataa
     4141 cagctcgttt cgcgcctggg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc
     4201 catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac
     4261 gacggcttta ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga
     4321 gttttcctca ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt
     4381 ttgcccctga ccgctgaagc caaagccgcc aacggtaaac accgactgga cattatcttt
     4441 ctctttagtc agataataat ccgtcacctg ttgcagcact ttcgtggtgt taaccatggt
     4501 ggcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa
     4561 agaggtcggc gtgcgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac
     4621 catgtagcga ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gttgagttga
     4681 tttttcaaac agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg
     4741 cgcggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga
     4801 aagcagcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcacc
     4861 gctcataaag gccatcggca taaacactgc ggaaagaaca acggcaatac cgaccagcgc
     4921 acgttggatc tgccccatcg atttatgcgt cgcttccttc ggcggtagct tatcttccgc
     4981 aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc
     5041 cagcaccatc ccgaacatag tcaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc
     5101 aaacgtcccg agaataacca ccggtacggc aatcgtcggg atgattgtgg cacggaaatt
     5161 ctgcaaaaac agatacatga ccaggaagac taggatgata gcctcaacca gtgttttgaa
     5221 aacttcctga atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag
     5281 acttgccggg aaataggctg ataagcggtt cagttcctct ttgactgccc gcgaggtatc
     5341 cagggcgttt gctccggcag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata
     5401 gcgcgccacg gtggaataat cttccgcccc aagttcgacg cgagcgacat cacgcagcag
     5461 cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg
     5521 cagacgcgtc tgcacaatga tcgaggcgtt tagctgctgg tctgccgcct gtggcatgcc
     5581 acccagttga ccaccggaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga
     5641 aggtaccagg ttatatttat tgagtttttg cgggtccagc cagatacgca tggcatactc
     5701 ggaaccaaag agttgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt
     5761 agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc
     5821 cgctaccatc aggatattac tgctcgactt atcgacgcta atcccctgct gctgcactgc
     5881 ttcaggtaat gaaggcatag cgagttgcag tttattttgc acttgaacct gtgcgatatc
     5941 aggagatgtc ccagtctcga aggtcagagt gatagaggca ttgcccgccg catcactggt
     6001 tgaagacatg tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac
     6061 cgagtcttct accgtttgcg catcggcacc tggataggta gcgctgacgg taatggtcgg
     6121 tggcgcaatc tgcggatact gcgcaaccgg taagttcatg atcgccagac cacctgcaag
     6181 catcataata atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat
     6241 gaaagtcccc ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct
     6301 ggaggaaatt gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac
     6361 ccgatcgcca gcctgcaagc cagaggtgac gacccactgg tcgccgatgg ctttgctggc
     6421 ttcaatttcg cgtagctgca cgacatcgtc tttatccaga atgagcgccg ttgctttacc
     6481 ctgggcgttg tgggtgacgc cttcctgcgg caccagtaat acattctggc ggctaccttc
     6541 atccactaat gccgtgacgt acatgccagg cagcaagtca ccatttgggt tggggaaaat
     6601 cgcccgtaac gtcacggagc ccgtggtttc atccactgtc gggtcggaga atttcagcgt
     6661 gccggtctgg ctgtagcgtt taccattttc cagattgagc tgtactggcg tactgccctg
     6721 aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat cttgcaccga
     6781 ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg aatctgcctg
     6841 attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg
     6901 cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg ctttggcgac
     6961 ggtgacattg gcttctgctt cattcaactg ggtgcgcgcg gtgtcgtaat cctgacggct
     7021 aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg cattgctggc
     7081 ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct gtaaaggtgc
     7141 aggatcaatc tgatacagcg aatcgccctg gttcacttta tcgccttcga taaagttgcg
     7201 tttaatgata ataccgccca cctggggacg tatctcggca acttcataag gaacggttct
     7261 accgggcaat tcgctcaaca cattgaccga accgggggag agtgtgacga caccgacctc
     7321 aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga gcatcgcgcc
     7381 gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc ctgaaaattc
     7441 ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat ttacagacca
     7501 gcggcatagt ggggttgtca gccagtaagg tgggatacag gcacagtgat cgacatggtg
     7561 aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc attcgggcaa
     7621 cgaagggttc tactggtgga tacacatacc aggggaataa agtcgtttta gcctctcctt
     7681 tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc agccatcaat
     7741 ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc
     7801 atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa tgtttgttgc
     7861 gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat cagttgcata
     7921 attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa gtgtttacca
     7981 taagtaatct caagaagatt tttgatactt tctttgcggc taacaaaata aataggcgct
     8041 ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc ggcatctaat
     8101 ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt atcaatgata
     8161 accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat ttttatcatt
     8221 tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga
     8281 aaaatcataa cttgctcctt agccgttatc gtttcgaata tgtcatcctt ttcattattt
     8341 acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt tacccttcgt
     8401 gaactaatac gtctattaac actaatcaat acttcagatt tatccatcac gcactccata
     8461 tattgatcct aagtaagatt cctggttgtt atcagcttgt aaatagcaaa caaaaagtta
     8521 acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga tctctgaaga
     8581 atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt ttcgacttaa
     8641 gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag taaataacgc
     8701 attcatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg caatgtaatc
     8761 aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata gcatcaacgc
     8821 ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc tgactacgga
     8881 aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa aaaaattaga
     8941 tgaaattcag taggttgaaa taatcactag caggttatta tttcaatgat agtgcgcaat
     9001 tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac ataaagtgac
     9061 tatgatccgg gtgacaaccg gggtaattat tgctgcttaa cgaacaaact ggcgaagctg
     9121 aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac cagcgttaca
     9181 gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag cacaccaata
     9241 acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat caggcgaata
     9301 acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat gcccagctcc
     9361 ggtgcgcgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag gaaaccggat
     9421 aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag cgcaataccc
     9481 gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac gaacgggaaa
     9541 ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat tgctctgcga
     9601 tgttttttaa gcatctccag atcaaacttc aaaattgttg ccttatctat atataacata
     9661 gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc aaaaacgcgt
     9721 ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg catctgtaac
     9781 tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg acatatacag
     9841 aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt
     9901 tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc
     9961 agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc
    10021 ctggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt
    10081 tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat
    10141 tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa
    10201 agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt
    10261 tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc
    10321 ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc
    10381 gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg
    10441 cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc
    10501 caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg
    10561 gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact
    10621 gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc
    10681 cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt
    10741 tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg
    10801 gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat
    10861 taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgatgtgc
    10921 tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg
    10981 tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga
    11041 cggcagcgga tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg
    11101 aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa
    11161 ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg
    11221 tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg
    11281 aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag
    11341 aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat
    11401 ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat
    11461 gctgatggcg taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt
    11521 tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca
    11581 tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acagccgctt
    11641 caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc
    11701 gcggaacaaa ggtcaccggc actttcccca ggcagcgttt aatattaaag ggggagaaaa
    11761 taatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat
    11821 tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt
    11881 cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga
    11941 acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt
    12001 ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat agttagctta
    12061 ctgtgtatta atttgatgta atgcatgatg cagatgctga gaaagcatct gcatctttcg
    12121 gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc
    12181 accccagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt
    12241 attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt
    12301 atacgggact ttattgataa agccaggttg ttcaccctga atggtgccga ggatggtaac
    12361 aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc
    12421 ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc
    12481 taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg
    12541 ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg
    12601 ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag
    12661 gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa
    12721 aaccataata gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt
    12781 aacgtaaacc tgtctgatgt ttatatcctt tctgtttagg tgaactcagt taattttggg
    12841 gcgtgacatg aatatccatc accccataat atagtgcgtt gttttaaaag tacataaaat
    12901 ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt atattctggt
    12961 aatgatttga taagcgaatt cttttaccag atggtatttc tatttccacc ccataattta
    13021 tatctttttc attaggcagt ggcctcttta aataaggcgt gtgtgacatt ttttttgggt
    13081 taagcgtctc gtattgaaat acttcgaatt gatattcaga catttctgcc catgtttgaa
    13141 ttttattgta tcgggagagt gtcgtaaagg ctttaacttg cttataacat tgtttttgga
    13201 attcttgctg atattttaat atgtcattgt atgaaagatt ggttatcctg gcctctaaaa
    13261 atttaattat tgtcgaagca atttccgctt ttgctcgtaa aaggttgtca ttaatggtta
    13321 ataatgttga aaataaacct tcgatttgtt tgtcaagata ttgtccatct ttatataatt
    13381 tgggggtagg gtgttcttta tgtaaaaaaa tagtgtcacc taaatttgcg tcagtgaaat
    13441 ttatattaag tagatcttcg aaataaaacc ttgacaaggt tagaacgaac ccggacaggt
    13501 tctgtcctgt catattgaaa tcaataaagt tggttccggc aaagactttc atcctgtttt
    13561 tattttcact gtgctcaata tataacttcg tcattttttg atacaggctt tgaatatctt
    13621 ttatattagt tggtaagtca gctcgttgtt cagcaataaa attagtatct gaaaacatgg
    13681 tttggacact gaatgttatg ggtattccct cttctacgct aattttagtt atgattgcct
    13741 tgtcgggttt aggattagcg acaacaatga ttcgacattc agcacttttg tttttttcat
    13801 atctaatgtt attaatctga ttaagtattg attgtgtctg ttgcgtaagt tgtgtcgaac
    13861 ttatcgtttc aatgttagta ataagactta caagttgagc cacaactttt gagtagtctg
    13921 catttgtata tgtagtgttc gtgtggcacg cattaatttg ttgaagttgg tgttgcgcta
    13981 aatcatattg atcagagata tatccttctc cagagaatgt ggaattaatg ggttgttgtt
    14041 cttcccaatt atcgcaaggc cacttcggtg tgtctggcgt tttagcatta tcggtatttg
    14101 ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta aagtcaattg
    14161 acataaaatt ccttattcat catttgtttt atgctaagat gtttaaattg gataattaga
    14221 acaggaatgg attttcttct acacacaata aatgtaatgc gttatatctt cctgatataa
    14281 ttacctttga tttccctttt atctccaccc ggtaatattt gtgtgtaaaa aaagggtagt
    14341 gatagtatcg taaaggaaat cgttaacaac tatcaaaatg cgctcatttt aaaaagttat
    14401 tatgttctct gatagtgtgt gaagtgattt tttttgtgat gttttagatg tgtaataata
    14461 aaatgggtta aggtatctta taattattta ataatttgtt gtgtaaacca ctgataaatc
    14521 ataatccagt acgtctttaa actactgcgc tgtatcgctg atacaccatg ctggacgcga
    14581 taattggaat ctcacgcagg gagtactgtg taaatagcaa aatcgcctga tgcgctacgc
    14641 ctgtcaggcc tacaaataac atactattca tcgaattttc ataactttgt aggtcggata
    14701 agacgcgcca gcgtcgcatc cgacataaat aaagcgcact tttctaacaa cctgtggggg
    14761 ggatatcgcc gctttatttc aggcgcttac ccgcttcatc aaccactttc tcgccatctt
    14821 ccttggagaa tgcgcctttt tgcgcatctg gcagaatttc cagcaccact tctgaagggc
    14881 ggcacaggcg agttcccagc ggcgtcacca caatcgggcg attaatcaga atcgggtgct
    14941 gaagcataaa gtcgattaac cgatcgtcag taaatttatc ttccgcaagg cccagctcct
    15001 catacggttc gacgttttta cgcagcagcg cgcgtacaga aatccccata tcggcaatga
    15061 gtttgaccag ttcatcgcgc gttggcggag tttccagata atggataatg gtcggttctg
    15121 tgccgctgtt gcggatcatc tccagcgtat tacgcgacgt gccgcaggcc gggttgtgat
    15181 aaatggtaat gttgctcata tcagtatctc attacaaagt gaaagagaga cgtagcgcca
    15241 gcgcagccag cgtcacaaac agcacaggca gggtcatgat aatccctgta cggaagtaat
    15301 atccccagct gatagtcata ttcttctgcg aaagtacgtg cagccagagt agcgtagcca
    15361 ggctaccaat tggggtaatt ttcggtccca aatcgcagcc aatcacattg gcataaacca
    15421 tcgcttcttt gataacgcca gatgccgtgc tgccatcaat ggacaacgcg ccaaccagta
    15481 ccgtcggcat attgttcata atagaagaga ggaaggcggt gaggaatccg gtgccgagcg
    15541 tcgcggccca caggccgtta tccgccagca cgttgagtac gccagaaaga tattccgtta
    15601 atccggcatt gcgcaggcca taaaccacca gatacatgcc gagcgagaag atgacaatct
    15661 gccagggggc accgcgcagg actttacccg tattaatcgc atgaccgcgt ttagcgacga
    15721 caaataatat cagcgcgccc acagctgcaa tggcgctcac cggaatgccg agcggttcca
    15781 ggacgaaaaa tcccaccagc agaagcagta aaacaaccca gccagttttg aacgtagcag
    15841 gatctttgat cgcttctgcg ggagatttca gcagcgccat atcgtagttc tgcggaatat
    15901 ctttgcgaaa atagagatgt agcatcacca gcgtggcaac aatcgcggcg atatccaccg
    15961 gcaccatcac cgaggcgtat tcgcgaaagc cgaggccaaa gaaatcagcg gaaacgatat
    16021 tcaccaggtt ggagacaata agtggcaggc tggcggtatc ggcaatgaat ccggccgcca
    16081 tcacgaacgc cagcgtagtg cctttactga accctaaagc cagcagcatg gcgatgacaa
    16141 tcggtgtcaa aataagcgcc gcgccatcat tggcaaacag ggcggcaacg gcagcaccga
    16201 gcaggacaat ccaggtaaac agcaagcgac cacgaccatt accccagcgt gagacgtgca
    16261 gcgccgccca ttcaaaaaag ccggactcat ccagcagcag gctgatgata atgacggcga
    16321 taaacgcagc cgtcgcgttc cagacgatat tccacaccac cggaatatca cccggatgga
    16381 ccacgcccgt aactaacgcc agtactgcgc cgagcgttgc actccagccg atgcctaaac
    16441 ctttcggctg ccagataacc aatacgatgg tcaggacaaa gatagcgcct gccagtaaca
    16501 taatgcctcc cggataaaac acatctgaaa attcatatgt gtttagctaa atttttaact
    16561 gcaaatgttc ttactgtccc cggaacagtt ttgtcgagcc aggttgcgga caattgcctg
    16621 aatcttttcc tgttcacatc gccaggcctc atcaataatt ttcgccgccc atgctggaat
    16681 atgcggtgat aagcggtaat gaacccactt accttgcttg cggtccagca atagcccgct
    16741 ttcacgcagc aatgccaggt ggcgggagat cttgggctgc gactggtcga gagcagtgca
    16801 gagatcgcag acgcataact ctcccagttc gctgagcagt aaaacgatgc ccagacgggt
    16861 ttcatcagca agaattttga acaattggat gggtaacaga aatgacatat tgcgctcctg
    16921 attgttgcag gtagtgtctc tcttcgaagc ggataagtca aaaacatata tgacttaacg
    16981 aatgtgtaag tgcagaggaa ggtaataggt gtgaattttg agttggctat tcatttgaaa
    17041 ggaggtctga atcaggacgt aaaaaaaccg actttgcgtc ggttttttac tttccagccc
    17101 tgagttggtg gctctggctg gagtgagaga gttcttatct aacagctcaa tactattagc
    17161 cagtggctaa cattgcaaga attagcgatt tcttcagctg gcggagggag cacttcggta
    17221 tatatcgcag tgacgattta tatactttca ctgggtcatc gtcatattaa gccttgtccg
    17281 aatccggatg taaaaaaacc gactttgcgt cggtttttta ctttccagcc ctgagttggt
    17341 ggctctgact ggagtgagat agccatcatc taacgcatca atgctaatac catactgaaa
    17401 ctattgcaag gacgtgctgg ttttataacc tgcatgtact gtatgattat ccagttagct
    17461 ctgaggcatt ttcactctgg caatgcgcat aaacgctttc aaagtcctgg tcagaagtac
    17521 gggtggtgcc gttaactgat gctctggccg gagtgagaga gttcttatct aacaatgaga
    17581 catgcgccgt gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag
    17641 aaaacgggtg attgataaag caatcatcgt tctaggggcg ttaattgcgc tgctggaact
    17701 gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc
    17761 acactactct tagcccttta acatttaacg cattgtcacg aactcttctg ccgccgttgg
    17821 gtgaatggcg acggtattgt cgaagtcttt tttggttgcc cccatcttca gcgccaccgc
    17881 gaagccctgc aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc
    17941 ttccgatcca acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt
    18001 atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccat actgctcgcg
    18061 cgcctgcggt tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg
    18121 aatgttgctg taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg
    18181 gcgacccgct gcaactgcta ccggtgtcag ttctactgca cccgtgttat cgccgaccgc
    18241 gtaaatacct tcaacgttgg tgttttgata tttatcgacg acgatatagc ctttttcgtt
    18301 ggtttttaca ccagcggctt ccaggttgat gttgtcattg gcaggctcgc gaccaatcgc
    18361 ccaaatcagg caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc
    18421 atcggcattt ttcactacca ctttcgggat ggcgttggta tgcaactgtg ggccttcggc
    18481 gttcatcact tcgaccagcg tttcagagat catcgggtcg aagctgcgca gcggcgcatg
    18541 tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta atcacgccag ccagctcaac
    18601 ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa
    18661 gccatcggaa tcaataccgt attccacgcc cggaatatcc gggtggctcg gacgaccgcc
    18721 ggtggcgatc agaatatgat cggccgtgat ggtttcaccg ttaacttcca gcgttttggc
    18781 atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc
    18841 ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt
    18901 gaatttattg atagtggtat caaaaccata atccgggccg tacatatgga tcgcttcacg
    18961 gatttgcgcc gcgtgccaca tcactttttt cggcacacag ccaacattta cgcaggtgcc
    19021 gcccagctct ttggcttcaa tcagcgcaca tttctggccg tacatagccg cgcggttgat
    19081 ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac
    19141 aaagtgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat
    19201 ggctgcaatt actccggcac gatccagctt acggtggcgt gcccggtgcc tgccggaacc
    19261 agtttgctgt gcagccacgg cagcacgtta ttcatctgtt gttccagttt ccacggcggg
    19321 ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagtaccgcc
    19381 agttcaattt gcagaatttt gcgaataccg gtcgcttcca gatcgtggat catgcgctta
    19441 atttgctgac gcagcaccac cggataccac agtgcgtaaa taccagtggc gaaacgtttg
    19501 taaccttctg ctatcccgct gaccaccgct tgatagtcag ttttcatttc atacggcggg
    19561 tcgataagga ttaaaccacg gcgggaaacc ggcggcagtt tggccttaag ctgctggaaa
    19621 ccgtcggctt tttcgacacg cgcacggcta tctttctgaa attcagaacg caacaacggg
    19681 taatcgctcg ggtgcagttc ggtcagttgc aggctgtcct gttcacgcag tagcaggcga
    19741 gcaatcaacg gcgaaccggg gtagtaacgc aactgaccgc tacggttgaa gtgttttacc
    19801 acattgatgt acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgatg
    19861 ccttcgagat attcgccggt acgctcggca tgttcgctgc ctaactgata acgcccggcc
    19921 cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc
    19981 aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga
    20041 taactgagca tgggtaaagg tgttccggta agtaaaaatc ggcccgtatt cggggcgcac
    20101 aaaagcgtat caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct
    20161 cattgaaatt cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc
    20221 gcttccttaa cctctttaac caggactgcg cgaatgacga atccgttact gactcccttt
    20281 gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggca
    20341 ttgaacgact gccgcgaaaa cgtggagcgc gtagtagcgc aaggggcacc gtacacctgg
    20401 gaaaatctct gccagccgct ggcggaagtg gacgatgtgt tggggcgtat cttctccccg
    20461 gtcagccacc tgaactcggt gaaaaatagc ccggaactgc gtgaagccta cgaacaaacc
    20521 ctgccgctgc tgtcggaata cagcacttgg gtagggcaac atgaagggct gtataaagcg
    20581 tatcgcgacc tgcgcgatgg cgatcattac gccacgctga acacggcgca ga
//
LOCUS       DACAPB010000071        20276 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.071, whole genome shotgun sequence.
ACCESSION   DACAPB010000071 DACAPB010000000
VERSION     DACAPB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.071"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1710)
                     /gene="feoB"
                     /locus_tag="IT904_002335"
     CDS             complement(<1..1710)
                     /gene="feoB"
                     /locus_tag="IT904_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAP3192107.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQA"
     gene            complement(1727..1954)
                     /gene="feoA"
                     /locus_tag="IT904_002336"
     CDS             complement(1727..1954)
                     /gene="feoA"
                     /locus_tag="IT904_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAP3192108.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(2392..4713)
                     /locus_tag="IT904_002337"
     CDS             complement(2392..4713)
                     /locus_tag="IT904_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP3192109.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(4810..5322)
                     /gene="greB"
                     /locus_tag="IT904_002338"
     CDS             complement(4810..5322)
                     /gene="greB"
                     /locus_tag="IT904_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAP3192110.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            5514..6233
                     /gene="ompR"
                     /locus_tag="IT904_002339"
     CDS             5514..6233
                     /gene="ompR"
                     /locus_tag="IT904_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAP3192111.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            6230..7582
                     /gene="envZ"
                     /locus_tag="IT904_002340"
     CDS             6230..7582
                     /gene="envZ"
                     /locus_tag="IT904_002340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAP3192112.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(7658..9280)
                     /gene="pckA"
                     /locus_tag="IT904_002341"
     CDS             complement(7658..9280)
                     /gene="pckA"
                     /locus_tag="IT904_002341"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP3192113.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            9659..11383
                     /locus_tag="IT904_002342"
     CDS             9659..11383
                     /locus_tag="IT904_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAP3192114.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLNVNLNE"
     gene            complement(11446..12324)
                     /gene="hslO"
                     /locus_tag="IT904_002343"
     CDS             complement(11446..12324)
                     /gene="hslO"
                     /locus_tag="IT904_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP3192115.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(12349..12750)
                     /gene="hslR"
                     /locus_tag="IT904_002344"
     CDS             complement(12349..12750)
                     /gene="hslR"
                     /locus_tag="IT904_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAP3192116.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(12761..13429)
                     /gene="yrfG"
                     /locus_tag="IT904_002345"
     CDS             complement(12761..13429)
                     /gene="yrfG"
                     /locus_tag="IT904_002345"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAP3192117.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLSVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(13494..15629)
                     /gene="igaA"
                     /locus_tag="IT904_002346"
     CDS             complement(13494..15629)
                     /gene="igaA"
                     /locus_tag="IT904_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAP3192118.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVTSFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDINIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANTGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            15949..16509
                     /gene="nudE"
                     /locus_tag="IT904_002347"
     CDS             15949..16509
                     /gene="nudE"
                     /locus_tag="IT904_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAP3192119.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(16677..19229)
                     /gene="mrcA"
                     /locus_tag="IT904_002348"
     CDS             complement(16677..19229)
                     /gene="mrcA"
                     /locus_tag="IT904_002348"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAP3192120.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            19349..20128
                     /gene="hofM"
                     /locus_tag="IT904_002349"
     CDS             19349..20128
                     /gene="hofM"
                     /locus_tag="IT904_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAP3192121.1"
                     /translation="MAFKIWQIGLHLQQQEALAVAIVRDAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     PGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
BASE COUNT         4598 a         5265 c         5457 g         4956 t
ORIGIN      
        1 tgcctgccag ttatcttcat gcacgccaat cggcttgaag actggggtga tcacccggct
       61 gacggaagcc agcgccgagt cgttgatgtt atcgacgatt tttccgctca gcgagaagct
      121 gttgaaggcg ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa
      181 gcctttcaga cgctgccagg tctggataat caggctttta acgtgtggta catggtagac
      241 cggcagctcc atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag
      301 gccagtcagc accgccatca cgatacccag catatacagc gagaaaaccg ccagggcacc
      361 gttctgcccg aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga
      421 cataaacggt gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc
      481 gcccattacc gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc
      541 aggcaagccc agcgcctgca tcaggcggtc catcacaaac gccgcacgcg ccatatagcc
      601 ggagtcctca aggaaggaga ggaacaggta catcatgcca atctgcggca ccagtggcag
      661 cacggtgtta ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa
      721 gtggagcgtg tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacatc
      781 aaacagcggc tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac
      841 aaagaggaaa atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt
      901 gaaacggctg ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagcaatgca
      961 ctggtaacgc gcatcggcaa tgtgcagcgc cggatcgtcc atctcattac gcagacgggc
     1021 gagggcggca tcaagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc
     1081 ttccagcatt tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac
     1141 ttttgccagt gaatctgctt cgttgagtag cggctgtgcg taatgtacca gttccacatt
     1201 ctcgttagct ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt
     1261 tgaaaccagc gggatcaccg gacagcccag acgcgccgac agagcatcaa tttcaatacg
     1321 aatattttgc ttctcggcaa tgtcgagcat gttcagtgcc acaatgcagg gaatgccgag
     1381 ttccagcagt tgtagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt
     1441 aatcagcagg tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga
     1501 ggtctgcgat gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg
     1561 atgatcggtg gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc
     1621 tacacgctga cgtgcgccag tgagctggtt aaataaggtt gtcttgccag aatttggatt
     1681 accaattaag cctatggtta attttttcat tgttgttatc accgtattaa caggaaaccg
     1741 cttccacttc taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt
     1801 cgatatgaat ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta
     1861 acatgccaag agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag
     1921 tgattttcca cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact
     1981 ggataactac ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacaaag
     2041 gagagattaa ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta
     2101 aaatgtgtgc gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt
     2161 tatatcgcag cgaaaatata attcgaatag tgaattaccc ggactggcta atgtttaatt
     2221 aaggtttcag ttgttaatga agtgataaat attgccggaa tatattatga gtttggttgt
     2281 ggaataaaaa ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc
     2341 agatgcggcg tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt
     2401 tttgcccatt gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc
     2461 acgaccgcgt ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg
     2521 acgggcgttg gtttcgccag gctgctcgtc cagacgcata gtcagggcga tacgtttacg
     2581 ctgaagatcc acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg
     2641 cggatcttcc acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac
     2701 gccgatatcg acaaacgcgc caaagttggt gacgttggtc accgcacctt cgaggatcat
     2761 acccggttgc aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc
     2821 cggacgcgga tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac
     2881 accgaatttt tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc
     2941 catcagatct ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc
     3001 ttccgggtgg acggtagaag catccagcgg gttatcaccg tggttaatgc gcaagaagcc
     3061 cgcgcactgc tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt
     3121 ctggaactgg ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag
     3181 gcccgccacg cgggttaaca gcggaacaga agcagtgttg agatcgacgc caacggcgtt
     3241 tacgcagtct tctactactg cgtccagttt gcgggccagt tgcgtctggc tgacgtcatg
     3301 ctgatactga cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc
     3361 ctgcaaacgg cgggcgatag acaccgcgcc acgcagcgaa acgtcgagat ccgggaactc
     3421 ctgcgctgcc agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt
     3481 ctgtgcggtc actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg
     3541 ggaagctgta ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc
     3601 aacggtcatc gctgcttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgctac
     3661 cagtttgcca gtggcatcga ccaccgccac ttttacccca gtacgcagac ccggatcgag
     3721 gcccatcgtt gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc
     3781 aaagacgttg attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc
     3841 cagatgcatc agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc
     3901 cgccggggca ttgttcaggc gtaggccaag gtgatccatg atgatttgct cgcaatagct
     3961 ctctttgggc ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc
     4021 gttacgccca cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg
     4081 atgatcgaaa tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga
     4141 aaccaaatgc gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc
     4201 ggcaaaccgt tccatcagga tatagcgcgc gccatccagc gcggctttgg tatctgccac
     4261 gcctttatcg gcatcaacat attgtgcagc ggcgacttct ggcgtgtgtg acggatcgct
     4321 ccacagcagg tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg
     4381 gcgtttaggt ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt
     4441 gatggccttc gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc
     4501 ctgacgtctc tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg
     4561 cgtgtcatcc agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt
     4621 cccttcgtca agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc
     4681 cgcaataatg cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa
     4741 aatcagggga tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa
     4801 gactcttcct tacggtttca cgtactcgat agcattaaca taccagctcg cttccccggc
     4861 aggggtattc accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg
     4921 ggaatcgata gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa
     4981 acggtgagtc acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc
     5041 ttcctgctga ggggagtaat cgacgatttt gagattttcc aggcatttag tgagatagcg
     5101 cacgcgacgg tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct
     5161 gcggtcgccc agacttgcgg cccaagtcac cttttttgtg acctccgggc gttcttcacg
     5221 ccagagataa ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt
     5281 tttcatctcg ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc
     5341 atatcaacag aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat
     5401 atgctttgta acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc
     5461 ttttttaaga atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag
     5521 agaactacaa gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt
     5581 atctcaccga acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc
     5641 tgctgactcg tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg
     5701 gcttgtcgat ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga
     5761 cggcgaaagg ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca
     5821 ttccaaaacc gtttaacccg cgtgaactgc tggcccgtat ccgtgcggtg ctgcgtcgtc
     5881 aggcgaacga actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt
     5941 tcaaacttaa cctcggtacg cgcgaaatgt tccgcgaaga cgagccgatg ccgctcacca
     6001 gcggtgagtt tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg
     6061 ataagctgat gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg
     6121 tgcagatttc gcgtctgcgc cgcatggtgg aagaagatcc agcgcatccg cgttacattc
     6181 agaccgtctg gggtctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt
     6241 gcgcttctcg ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt
     6301 cgccagcctg gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca
     6361 gcagtttaat aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact
     6421 ggaggacggc acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct
     6481 ggggatctct ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca
     6541 ctatgaattc ttaagccatc agatggcgca gcaactgggc ggcccgacgg aagtgcgcgt
     6601 tgaggtcaac aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg
     6661 ggtacgcgtg ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac
     6721 gctggcgatt atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg
     6781 accgttggtc gatctcgaac acgcagcctt gcaggttggt aaagggatta ttccgccgcc
     6841 gctgcgtgag tatggcgcgt ccgaggtgcg ttccgttacc cgtgccttta accatatggc
     6901 ggctggtgtt aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga
     6961 cttgcgcacg ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg
     7021 ctatctggca gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt
     7081 tatcgactac ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt
     7141 actcggtgag gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta
     7201 ccccggcagc attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat
     7261 ggtggtcaac gccgcccgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc
     7321 gaatcgcgcc tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa
     7381 gcacctgttc cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt
     7441 agggctggca attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac
     7501 cagcgagcgg ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca
     7561 gggcacgaca aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct
     7621 ataagatact ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta
     7681 ccagcgcggc acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg
     7741 ccagggtttc ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga
     7801 gaatcttcgt gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag
     7861 tgaaggtttc tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat
     7921 ctttaatcga gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg
     7981 cgcccgccgc ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca
     8041 ggaatgccgc gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag
     8101 taccggccag tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca
     8161 ggcgagaaac cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg
     8221 cgtggcccgc tttggaaacc ggcttaacaa tgttatcgat gtgatagatc ggataagaaa
     8281 cgcgggtgtt ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg
     8341 tgacgttttc cagcaacgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt
     8401 tcgacagctt gatagttttt gcgtagcagc cgccttcgaa gttaaacacg ccgtcatcgt
     8461 cccagccgtg ttcgtcatcg ccaatcaggc gacgtttcgg gtcggtggaa agggtggttt
     8521 taccggtgcc ggaaaggccg aagaacaccg caacatcgcc tttctcacca acgttggcgg
     8581 agcagtgcat agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc
     8641 ctttcttcat ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt
     8701 taaacgccac gaagttttcg gagttgagac cctgttcttt ccactgcggg ttagtgcact
     8761 tcgcgccgtt cataacgata aagtctggtt tgaaacctgc cagttcttca tcgctcgggc
     8821 gaataaacat gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg
     8881 aaagacgagt atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg
     8941 aaagctgcct ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt
     9001 tgtcgttctt acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac
     9061 ggacgatata cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg
     9121 cacccagatt agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct
     9181 gatacagcag gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag
     9241 cctcgagttc ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat
     9301 atgtattgcc tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat
     9361 ttacgtatct ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg
     9421 ttgctattat tgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct
     9481 tggtttgtat ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa
     9541 ttcaaccggt aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag
     9601 caaaatacta ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat
     9661 ggataacgtt gaactttcac ccgcaacacg ctgggggatg attgctaccg gattacttca
     9721 gggtctggtt tgctacctgc tgatcgcctg gctttccgga aaaaatcaca gttggattgt
     9781 ttatggcgtg cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt
     9841 taaacagaag cgcctttggg gatggctggc gctagtgttt attgccacgc tggggatgag
     9901 cggctggctg aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgctttg
     9961 ggattttggt tgctatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag
    10021 ccttcgcatc cgcaatgaca gtagccgcta tcgctatttt taccagtcag tatggcataa
    10081 cgtacttata ttactggtga tttttctcgc caatggcttg acgtggctgg tgcttttact
    10141 gtggagtgag ttgtttaaac ttgttggcat cacgtttttc aatacgctct tttttgcaac
    10201 cgactggttt atttatctca cgttaggtct ggttaccgcg ctggcggtga tcctcgcgcg
    10261 aacacagtca cgtttaatcg actctattca aaagttgttc acgttaatcg ccacggggtt
    10321 gctgccgtta gtatcattgc taaccctgat gtttatcatc accctgccgt ttacgggcct
    10381 gagcgcgatt tctcgccaca tctccgccgc cgggttgctg ttgacgctgg cctttttgca
    10441 attgatctta atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacagggcc
    10501 gttgcgttgc ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc
    10561 ctgggcgtta tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgt
    10621 gctggcggtg ctggtgttac tggtgtggtc gctggggtat tttgtcagca tcgtctggcg
    10681 taaagggcaa aatcccgttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt
    10741 gttggtgata ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa
    10801 cagccatatg gcgcgttatc agagcggcaa aaacacgtca gaccaggtaa ctatctacat
    10861 gctcgagcag agcggtcgct atggacgtgc tgcgcttgag tcgctgaaaa gcgatgccgg
    10921 gtttatgaaa gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca
    10981 tcttcagcaa caggtatcgg aaaaagtatt agccgataat gtgttaattg cccctggttc
    11041 tgttaaacct gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac
    11101 ctgtattgaa aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc
    11161 ggagcggatc ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga
    11221 cagaaaagaa tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa
    11281 attactcaca gctgcgaagg atggaaaact ggggacgaag cctaaagcgt ggcgcgatct
    11341 tgtagtggat ggtgaaaggc tgaatgtgaa tctgaatgag tgacaaagcg catcaggtgt
    11401 attagtcgta aaatccggca gagccctctc tgccggacat actcattaat gaacttgcgg
    11461 atctgccgga gacgcgttgt tgcggatttc agcaatatcc atcgcattga acagatagtg
    11521 gttaccgcag taatcacaat gcatgtcaat ttcgccatct tccgccagga tgctatcaac
    11581 ttcttcatca ggcagcgttt tcagcgcatc ggcgcaacgt tcacgcgagc aggtgcattt
    11641 gaactccaca tcctgcggat cgtaaaccgt cacctcttct tcgtgataca aacgccacaa
    11701 cacttcgttt gccggtaagg tcagcagttc ttcggttttg atggtttcgg ttagcgtcgc
    11761 caggtggtca aagtcgtcct gctgggcatt ttgcgcaggc attacctgca acaacatacc
    11821 gcctgcagcc ggtttgccgt ctacgtcgcc ggtgcgaata aacaggcgcg tcggcagctg
    11881 ttcagaacgc ataaagtaat cttccaggca ggccgccagg gtatcacctt ccagaccaac
    11941 tacgccctga tagcgttcgc cttcgctcgg ggtaatggtg atcaccacgt aaccattgcc
    12001 gaccagcgtt ttcaggtcgg cattttctgg aatttcgccc tgcacgcgcg ccacaccgcg
    12061 catctgctgg ttattgttac cgttaataac cgccagattc atcggaccgt cgccctgcag
    12121 ctgtacggtg atatcaccat caaacttcag cgtagcggtt aacaggctgg tcgcaaccag
    12181 cagttctgcc agcacgtttt taacgggctg cggataatcg tggttctcaa ggatctgttg
    12241 cagggtttcc gaaacggtta ccagttcgcc gcgcacggca aagttttcaa acagatagcg
    12301 atgtaattgg tcatgttgcg gcataatcat ctctcttgca ggtgacagtt attcactgtc
    12361 gccgtgttta aatcgtaaca ggtcgcggcg ctcttttttg tccgggcgtc ggtccgggtg
    12421 cggcatggtt aaggcattaa gtttacgtgc cagcgccatt ttttcgcgtt tctctacact
    12481 ttccgcagtc tcttcataca gcaaggctgc ctcgctggcg gggcgacgct gttcagtaat
    12541 cgcctttaca atcaccgtgc gttcgtcatt tccctggcgc agagtgagcg tggcattcag
    12601 ctcgacgatt ttgctcggct tgctgcgctg cccgttgtaa tgcaccttac cgccttcaat
    12661 catttcacgg gccagcgcgc gggttttata aaaacgggca gcccatagcc atttatccag
    12721 tcgaacctca acagcaggtt tttctttcat ggcgtctcct tcacattagc gaggggatca
    12781 ggcggcggta gtcattcagt gacggatggc gttgatactg tttctcggca atcccggaat
    12841 caggattagt cacgccgagg cagtaacgaa taccaaattg cgcggcagca tcgagaatcg
    12901 cttcgctgtc atcaataaac agcgttcttt cagctttcag acccgtagct tcggccactg
    12961 catgccataa ccgctgatcc tctttcggat aaccaaatgt gtgggtggaa agtaataaat
    13021 caaggtgtgc gtccagaccc gtatgctcaa gttttaccga caggttgtgc ggatgcgcat
    13081 tggtgagcaa aattcgctgc ttaccgctgg ctttcagtgc ctcaagaaac ggaatggtat
    13141 cttcacgcag tacggcacgc ggtcccatct cggtggtcat cgcacagata tccagaccca
    13201 gttgctcact ccagtaatca agacagtacc agtttagcgt atgctgtacg tcgtgatatt
    13261 gctggcgcat atattccatc gcttcctgtg gcgtaacccc gtttttcgcg ccccatgttt
    13321 caggcaccag cttttgccag aaatagttat cgaaggcgag gtcgagcaac gtgccgtcca
    13381 tatccagcag aacggtatct acgtcctgcc aggcaatgtt gatatgcatg agggaaatct
    13441 ccagagtgaa gcaatttgcg cgacagggta gcataacctg ccgcgcaaac gtgttattcg
    13501 ataaggcttt ctgaaggggt gatcagttgc gggttcaggc agctttcata ataggcctga
    13561 atctccatca tgcgagtgcg atgacgctgg taacgacgcc aggcctgtac gccattgtaa
    13621 atggcgctac ccagcatcgt cagtagcagc aatgtggtgc tgagatagcg ccacaggccg
    13681 gaacgatccg ggatcggatg aaggccaata tgctgcgtac cattggcgtc agtgaagatt
    13741 tttgtgacga taccctcggc gttaaacggc gtatgcatca gcatttgtgc cagtttctgg
    13801 aaagcgttcc actgttcttg cggcgggtag tcgtaaagtg atgccgaagg ccagggctga
    13861 tcaacaaaat cgctgccttc gtcgctgaca atcaggaatc cgccgggggc cggactgttt
    13921 agtgattgtg ccgctcgcgc cgtttcatgc gtgataaacg gcgcggtgga ggttgccacc
    13981 aagttatcca gcgattccgc actcaccggg cgtaataaca cattcacgcc atcgagcttc
    14041 ccggtgttgg cgcgttttac cagcgcgtcc cagtctttac tgttgccgag attgaccagc
    14101 gcatttttca gtcgcacaca gtcatctttg gcagaacata aatccgctgt cttcagtaca
    14161 atgtcgccaa aatcatcaag caataccatg ccggattttt gaatcgctga acgtaatgag
    14221 gcactgacgc gagattcatc ttccggttta gggtgcagct ggcgattaac tgcttcggtc
    14281 aatgccgtcg ctttgttgac cagttcagat tctggtaatg gtaatgagcg ggcgtcgttc
    14341 cagatgatct gcgagcagtc aaacggtaaa aaaggtgaat tggttttcgc gctccaggtt
    14401 ccggaagtgc gaatattaca cattcccgta ccgctaatac gcaatgtatc gcctacccgc
    14461 acgccagcgt cagccagttg ttttacgctg gtggcttcaa tggtctgcgc gcctttcatc
    14521 catgagagag tgaatttcag cggcatatcc agcgggatcc agaataacag cataaacagc
    14581 accagcagcg agcccgcagc gataatcgta ctgcgcagcc agtgctgtaa cggaaagttt
    14641 tttacttcat catgcagcga aagatatcgt ccctgacgca ctacatggcg gtcgagatag
    14701 atattgatat cggtttgttg accgagatcc tgagcaatgt atggctgcca gtgtgcggga
    14761 tagaccaggt cgataatacc gagtgaaata ttgttgatct gttcctgatc gttttcgcca
    14821 aacaatcccc aacgacgggg tgtaccgcgc agacaatgaa tttcccgcag ggaggatttt
    14881 gccgggggcg cgaatagccc ccacagacct gcgcccagca gcagtaacgc gccgcctgcc
    14941 agccacggaa caaatacatc cggggtaatc aggcagaaaa agaacatcag gaaggaggtg
    15001 acgatcagca acgcttcacg cagcccgcgc ggacgactca gcgcatattc ttcatgcgtt
    15061 tctttgcgaa tattgagtag ctcgatttgc tcactctctt ccccgcgaat cgacgcctgc
    15121 gttgacggaa caggttgcaa ggcatagccg cgagtttcct gcatgtactc ctgcagcgta
    15181 tgaccgttga gtgaaataac cagcggcagc gaatcggtat gaatcagttc aacggtattc
    15241 tcatcgttga tgtactgttc ccagaaaggg ggcaggtgga cttctaccga atcgaggtag
    15301 taacgccatt tattcggatc gtcggtagag atgccgtaac gcgtgatagc gtgtgtcagc
    15361 atcatgacgt tgttgctttc ggcattcagc gccagagaga tcggtgccgc gctggctccc
    15421 gttgggccag gcacctgtaa tacctgcgtc aggctctcaa gataattttc aacggcgcta
    15481 cgttcttcag gtgtgagttt acgcgtttgc gcatccgcga aggcgttggt ccagggcagc
    15541 tgacgccgtc tggatcgcac ttttatcagc catcccgcaa gtagtgagca ggccagcaaa
    15601 gcagctaaaa aaatcacaat ggtgctcatg ctttccccat cttacttagt ttgtcaggcg
    15661 tggtcagtcg caccggacac aatacatgat tctgcggttg gctatcggca agcgtggagt
    15721 ttaacttgag catcattaag cacatcacag taaccgctga tcatagcaaa ccctctgaag
    15781 gcggaatatc agcaaattcc tggagttatt tcaacctatt gacttttaat ttgatttgag
    15841 gacgattatt tattaagtta cataaatgta aataacatgc aattcacatt gccgaaagcg
    15901 tgtccgatat cgcacaatag ccgctgattt cacagcatag atcccacgat gagcaaatca
    15961 ttacaaaaac ccaccattct gaatgttgaa actgtagccc gttcccgact gtttaccgtc
    16021 gagagcgtgg atctggagtt cagcaatggc gtgcggcgtg tttatgaacg aatgcgtcca
    16081 accaaccggg aagcagtgat gattgtgccg attgtggacg atcacctgat cctgatccgc
    16141 gaatacgcag tgggaactga atcctacgaa ttaggttttt cgaaaggatt aattgatccg
    16201 ggtgaaagcg tctacgaagc cgctaaccgc gagctaaaag aagaggttgg atttggtgcg
    16261 aacgatctga cttttttgaa gaagctcagc atggcaccgt cttatttttc cagcaaaatg
    16321 aatatcgtgg tagcgcaaga tctctacccg gaatcactgg aaggcgatga gccagagccg
    16381 ctaccacagg tgcgctggcc gctggcgcat atgatggatt tgctggaaga ccctgacttc
    16441 aacgaagcgc gtaatgtcag cgcgctgttc ctcgtgcgcg aatggttgaa agggcagggg
    16501 cgagtgtaaa tgcgtaaagt tcgcctgatg cataatgctt atcaggctta cgaagtttct
    16561 gcaatatatt gatttttcta gatgttgcta gacgggtaaa gtgttcacgc cgtatccggt
    16621 ataaacaaag tgcactttgt cagcaaactg aaaaaggcgc cgaagcgcct ttttaatcag
    16681 aacaattcct gtgcttcgcc attatcgata atggtcgtac ccacctcgtg caccgcttgt
    16741 tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc tgttgccacc attagctaac
    16801 tgcccggtgc tgcgatcgat attcaccgtc acaatacccg gtggcggcgt tagcggctgc
    16861 tccggcacac cttcaagaac ggctttcata taagcgtccc atgcaggctg ggcactcttg
    16921 gcaccgcctt cgtaacctga gatctgatct ttaatcgctc cggaagccgt tgtatgaccg
    16981 agattacgac ggtgatcatc aaagccaatc cagaccgagg tcacaacacc cggaccgtaa
    17041 cccgagaacc acgcatcttt cgaactgtta gtggtaccgg ttttcccgcc gatatcgcga
    17101 cgctgcaaat cacgacctgc acgccagccg gtaccctgcc agcctggctc accaaagata
    17161 ttggtgttca aagcactctt aatcaggaat gccagcggag tgttgatgac gtgcggtgcg
    17221 tactcctgcg cgccagtctt cgccactaac gcctgattag cctgctccag ctgcggcatt
    17281 ggtacagaaa cattctgctg ctcgcgggag atagcgacat cttcaacatc gttattttcc
    17341 agcacgttcg atttctgcgt atcaccgtaa atcaccggaa tatcacattc cgggcaagct
    17401 actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt tttcaatttt gctgataaac
    17461 cacgggtcca ccaggaagcc gccgttcgcc atgaccgcgt agccacgcgc cacctgcatt
    17521 ggggtaaagg acgctgaacc cagcgccagc gattcggtgt ggacaatgtt ttgcgccggg
    17581 aagccgaagc gttgcagata ttctgcagcg tagtcgacgc ccatcgcccg cattgcgcgt
    17641 accatcacca cgtttttcga ctgacccagc ccctgacgta agcgaattgg accggcatac
    17701 tgcggtggtg agttcttcgg ctgccagtca gaaccggcac ctgcatccca gcgggaaatt
    17761 ggcacatcgt tcaacatact tgccagcgtc agacctttat ccatcgccgc ggtgtagagg
    17821 aacggtttga tgttggaacc cacctgacgc agtgcctggg tggcgcggtt aaacttgctc
    17881 tgattgaaat caaagccacc gaccagcgcc ataacggcac cattttgcgg attgatcgac
    17941 accagcgccg agttcacttc cggcacttgt gccagccacc atgcatcgcc gacctgacga
    18001 acccagattt gctgtcccgt ttgcagaaca tcggtcactt tacgcggcgt cggtccctgc
    18061 tgagtatccg aacggtaagg acgcgcccag cgaacgccgt ccatactcaa tgcgacagtc
    18121 gacccgtccg ccagcattgc cgtcgcttcc tgaggattgg cgctggtgac tgcggcaggc
    18181 agcagcggac cataggtcgg cagcgccttc agcgtatcgg taatcttgtt gttatcccac
    18241 gccgactcgc ccactttcca cagcacattt gccgggccgc gatagccgtg gcgcatgtcg
    18301 tagtccagca cgttattacg taccgcctgc tgcgcggcct gctgcacttt gcgggtgatg
    18361 gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc cataacggtt atacatctcc
    18421 tggcgcacca tttcgctcag gtacggcgca gagaaagcaa tctccggcgc gtgatagtta
    18481 gcgttaatcg cctcagtgcg tgtctgatcg aactgttgtt gggtgatata cccttcatcc
    18541 agcatccgcg acagcacgac gttacgccgc gcgacggcac gatccatcga gtagagcggg
    18601 ttgaaggtgg aaggcgcttt cggcagcccg gctatcaccg ccatttcgtt cagcgtcagt
    18661 tggtcgaccg tttttccgaa atagacttgt gccgcagcac cgacaccata ggcgcggtaa
    18721 ccaaggtaaa tcttgttcag ataaagctcg aggatctcgt ctttcgtcag cagctgttca
    18781 atgcgaatcg cgaggaagac ttccttaatc ttacgcatca gcgtgcgttc tggactgagg
    18841 aagaagtttc tcgccagctg ctgggtaatg gtacttgccc cttgtgacgc gtgaccggag
    18901 aacagcgcca cgcttgctgc acggaagatc cccaccgggt caacgccgtg atgctcgtag
    18961 aagcggctgt cttctgtcgc gataaaggct ttcaccatct ccggtgggat ttgatccaac
    19021 gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca gctcgccatc ggcgctgtaa
    19081 atctgcatcg gaatttgcag gcgaacatct tttaatgtcg ccacatccgg cagttgtggc
    19141 tcgatgtagc ggtataggcc ataaatcgag cctgctccca gcagaatgca acagactgca
    19201 aggatcaaaa aatactttac gaacttcact ggaaatttcc catttagttt catttgggca
    19261 gtttataaac aaacgcgcgg tagtataaag gcaagccaga cgcattgata tacccgtcag
    19321 agtgacgggt gataaggaga tcatcacaat ggcatttaag atctggcaaa ttggtttgca
    19381 tttacaacag caagaagcgt tagcggttgc gatcgtgcgg gacgcaaaag aatgcttttt
    19441 gcaacgctgg tggcggttgc cgttggcgca cgacattatc aaagatgggc ggattgttga
    19501 tgcgcagcgg ctggctaaaa cgttgttgcc gtggagtcgc gaactgccgc agcgccatca
    19561 catcatgttg gcgtttcccg ccagtcgcac attacagcgg tcatttccgc gcccgtcgat
    19621 gtccctcggt gagcgggagc aaatggcctg gctgcctggg acgatggccc gcgagctgga
    19681 tatggatccg gactccctgc gcttcgatta cagcgaagac tcactcagcc ccgcttataa
    19741 cgtgactgcc gcgcaaagca aagagctggc aacgctactt actctggcag aaaggttgcg
    19801 tgttcatgtg agcgcgatca ccccggatgc cagtgcatta cagcaattcc tgcctttttt
    19861 accttctcat cagcaatgtc tggcctggcg tgataacgaa cagtggctgt gggcgacgcg
    19921 ctatagctgg gggcgcaaac tggcggtagg gatgactagc gcgaaggagc tggcggcagc
    19981 gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa ggcggatttg atccctggga
    20041 ggccgtttct gttcgtcagc cgccgctacc gccgcctggt ggagactttg ccatcgcgct
    20101 ggggctggcg cttgggaagg cgtactgatg aacccgccaa ttaatttttt gccctggcga
    20161 cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga tgttcgttgc gcctctgctg
    20221 ctggccgtcg ggataacgct aatactgcgt ctgacaggca gcgccgaagc gcgcat
//
LOCUS       DACAPB010000072        20159 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.072, whole genome shotgun sequence.
ACCESSION   DACAPB010000072 DACAPB010000000
VERSION     DACAPB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.072"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1198
                     /locus_tag="IT904_002350"
     CDS             <1..1198
                     /locus_tag="IT904_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAP3192122.1"
                     /translation="LPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALES
                     IEECWFARHLLACFYYNNRSYNKAIAFWQRCVEMSPEFDDGWRGLAIHAWNKQHDYEL
                     AARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELL
                     NLWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASEL
                     LHAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIH
                     SYYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDL
                     LSLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPF
                     IFNYVH"
     gene            1295..2278
                     /locus_tag="IT904_002351"
     CDS             1295..2278
                     /locus_tag="IT904_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP3192123.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            2301..3812
                     /locus_tag="IT904_002352"
     CDS             2301..3812
                     /locus_tag="IT904_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP3192124.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            3837..4835
                     /locus_tag="IT904_002353"
     CDS             3837..4835
                     /locus_tag="IT904_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3192125.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            4901..5962
                     /locus_tag="IT904_002354"
                     /pseudo
     CDS             4901..5962
                     /locus_tag="IT904_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417040.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            5974..6846
                     /locus_tag="IT904_002355"
     CDS             5974..6846
                     /locus_tag="IT904_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP3192126.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(6894..7316)
                     /locus_tag="IT904_002356"
     CDS             complement(6894..7316)
                     /locus_tag="IT904_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAP3192127.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(7413..8615)
                     /locus_tag="IT904_002357"
     CDS             complement(7413..8615)
                     /locus_tag="IT904_002357"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAP3192128.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(8625..9437)
                     /locus_tag="IT904_002358"
     CDS             complement(8625..9437)
                     /locus_tag="IT904_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAP3192129.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(9434..9754)
                     /locus_tag="IT904_002359"
     CDS             complement(9434..9754)
                     /locus_tag="IT904_002359"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAP3192130.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(9754..10272)
                     /locus_tag="IT904_002360"
     CDS             complement(9754..10272)
                     /locus_tag="IT904_002360"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAP3192131.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(10269..11630)
                     /locus_tag="IT904_002361"
     CDS             complement(10269..11630)
                     /locus_tag="IT904_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HAP3192132.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            11766..12656
                     /gene="hcaR"
                     /locus_tag="IT904_002362"
     CDS             11766..12656
                     /gene="hcaR"
                     /locus_tag="IT904_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAP3192133.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            12816..13955
                     /locus_tag="IT904_002363"
     CDS             12816..13955
                     /locus_tag="IT904_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAP3192134.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLIFALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13947..15227)
                     /gene="csiE"
                     /locus_tag="IT904_002364"
     CDS             complement(13947..15227)
                     /gene="csiE"
                     /locus_tag="IT904_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAP3192135.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGMTPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(15418..16272)
                     /locus_tag="IT904_002365"
     CDS             complement(15418..16272)
                     /locus_tag="IT904_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP3192136.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYHGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NTLAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(16417..17220)
                     /gene="suhB"
                     /locus_tag="IT904_002366"
     CDS             complement(16417..17220)
                     /gene="suhB"
                     /locus_tag="IT904_002366"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAP3192137.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            17339..18079
                     /gene="trmJ"
                     /locus_tag="IT904_002367"
     CDS             17339..18079
                     /gene="trmJ"
                     /locus_tag="IT904_002367"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAP3192138.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            18440..18928
                     /gene="iscR"
                     /locus_tag="IT904_002368"
     CDS             18440..18928
                     /gene="iscR"
                     /locus_tag="IT904_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAP3192139.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            19169..>20159
                     /gene="iscS"
                     /locus_tag="IT904_002369"
     CDS             19169..>20159
                     /gene="iscS"
                     /locus_tag="IT904_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP3192140.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTS"
BASE COUNT         4705 a         5056 c         5594 g         4804 t
ORIGIN      
        1 gctgccgctt tacctgcaag ccagcttgct gccgaaagcc gaacgtggcg aactggtcgc
       61 aaaagccatt gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc
      121 ggcgctggag agtattgaag agtgctggtt tgctcgccat ttactggctt gcttctacta
      181 caacaatcgt agctacaaca aagccattgc cttttggcaa cgttgcgtag agatgtcgcc
      241 ggagtttgac gacggctggc gcgggttagc gatccatgcg tggaataagc aacacgatta
      301 tgagctggcc gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct
      361 gcttttcgaa cgggatttgc ttgataagtt aagtggagcc acaccggaga aacgactggc
      421 gcgtctggaa aataatcagg aaattgcgct gaaacgcgac gacatgaccg cagaactgct
      481 caatttgtgg catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgtaa
      541 attccacccg tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt
      601 attacgcgcc tggcagcatc ttgatgccag agagccgcag caggccagcg aactgcttca
      661 tgccgcgctg cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa
      721 cgacatctgg ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgat
      781 gcgttgttta cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa
      841 cgatcagccg gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggtgaaca
      901 gcaaaccgca cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac
      961 cagtattgag gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga
     1021 tttacaacag cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact
     1081 cggggaggtt gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg
     1141 gccgaaagcg gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac
     1201 cctattaact gtaatatttg agcggcatgc gctgccgctc atcacaaaat aattagaata
     1261 agccgttgtt aataaaaacc ctacaggagg cactatgcct aaaaaaatga gaacaacccg
     1321 taatctatta ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa
     1381 agaaatgacc attggtgcaa tataccttga tacccaggga tattacgctg gagtgcgtca
     1441 gggcgttcag gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc
     1501 ccagggtgat atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga
     1561 tgccattatt ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag
     1621 tgaagcgggt attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa
     1681 atatgtctcg gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc
     1741 tgccgccgat tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg
     1801 cgaagccttt gaagtttgtg tgcaacgacg taaagggttt gaagaagtat taaaagcccg
     1861 cgttcccggc gcgcaaattg tcgctaatca ggaagggact gttttagata aagcgatttc
     1921 cgttggtgaa aaactgatta tctccacgcc ggatctcaac gccattatgg gggaatcggg
     1981 cggtgcgaca ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc
     2041 tgttttcggt tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct
     2101 gaaagcggta gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt
     2161 gaaggttatc aataaacaag ccgacggtga aaaagtgatt caggtgccta tcgatctcta
     2221 taccaaaacg gaagacggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc
     2281 gctgattcgc agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg
     2341 tggcaggaaa taagcgttat cccggcgtcg ttgcgttgga taacgttaac ttcacgctca
     2401 ataaaggcga agttcgtgcg ctgttaggca aaaacggcgc gggcaaatcg accctcattc
     2461 gaatgcttac cggtagcgaa cgtccggata gcggtgatat ctggattggc gagacgcgac
     2521 tggaaggcga cgaagctacg ctgactcgcc gtgccgctga actgggggta cgcgcggttt
     2581 atcaggagtt aagtctggtg gaagggctga cggtggcgga aaacctctgc ctcggtcagt
     2641 ggccccgccg caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct
     2701 tacaggcgtt gggcgttgac gttagtcctg aacaacttgt ttcaacacta agcccggcgc
     2761 aaaagcagct ggtggaaatt gcgcgggtga tgaagggcga gccgcgcgtg gtcattcttg
     2821 atgaacctac cagttcgctt gccagtgcgg aagttgaact ggtgatcagc gcggtgaaaa
     2881 agatgtcggc actgggcgta gcggtaattt atgtcagcca ccggatggaa gaaattcgcc
     2941 gcattgcctc ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg
     3001 aaaacacctc cacgcatcat attgtgtcgc tgatgctcgg gcgcgatcac gttgatattg
     3061 cgccggttgc ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc
     3121 ataagcccaa gctggaggat atcagtttta cgctacgtcg tggcgaagtg ctcggcattg
     3181 ctggtctgct gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggaggagt
     3241 atgaacaggg cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca
     3301 tgctgaaacg cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct
     3361 ggttgggcgt tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag
     3421 tgctgcaatg gtccaccatc cgccgcctga ccgaagaagt gatgcagcgg atgacggtca
     3481 aggccgccag tagcgaaacg cccatcggca cgctttctgg cggcaatcag caaaaagtag
     3541 tgatcggtcg ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg
     3601 gcgtcgatat cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag
     3661 gaaaaagcgt ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca
     3721 tcctgttatt acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg
     3781 agctgatgtc cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt
     3841 ctgcttcgtc attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc
     3901 gccatattaa tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc
     3961 tgaacgcgcc tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca
     4021 ccattgggat tgccgcctgg gcgatgacgc tgattattat ctccggtgag attgatgtca
     4081 gcgttgggcc gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag
     4141 ttccgttggc gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg
     4201 ccggggtgct gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga
     4261 gcgccctgcg cggaatgggg ctgtttatga cgaacgcact gccagtgccg attgacgaaa
     4321 acgaggtgct ggactggctg ggcggacaat ttctcggtgt gccggtatcc gcgctgatca
     4381 tgattgtgtt gtttgcgctg tttgtgttca tcagccgcaa aaccgccttc gggcgctcgg
     4441 tttttgccgt tggcggtaat gctacggcgg cgcagttgtg cggcatcaac gttcgtcggg
     4501 tgcgcattct tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgttgg
     4561 cggcgcgcct cggttctggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg
     4621 ccgcggtcgt ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat
     4681 tgcttggtgt gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact
     4741 cctttttcca gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct
     4801 tgctgaccca gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc
     4861 ggcagggatg aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc
     4921 ttatttacca ggaaattcga ccgtcgatct acgggaagtt gcggtgccga caccagggat
     4981 taactaggta ctgatcaaaa tgaaatcttc cggaatttgc ggaagcgatg tccactatat
     5041 ctaccaccag caccgcgcta cggcagcggc acccgacaaa ccgttatacc aaggatttat
     5101 caacggtcat gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa
     5161 agagggcgac cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg
     5221 ccgcggcttt cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga
     5281 cggcggtcat gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc
     5341 gctgagctac gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat
     5401 tttgcgcggc gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg
     5461 catgatggcg atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat
     5521 gctgccggaa cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc
     5581 aaccaccgaa ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc
     5641 gctcgattgt tccggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg
     5701 gggacgggtg gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct
     5761 gatgcaccat caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa
     5821 atgcgcccat gatctgacgg actggaagct gtggccgcgt aacgccatta cccatcgctt
     5881 ctcgctggaa caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt
     5941 tgtgattaac ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg
     6001 tcgctaaagc tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg
     6061 acgccgctct tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg
     6121 ctggtgccat ttgcaaaccg ggtgagcggc aatcgttttg tctggcaggg gcgtgagtat
     6181 caactgcaac cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc
     6241 gagtggcaat gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc
     6301 ggtgtctatc actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg
     6361 acgctctctg tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct
     6421 tattttccgt tgtcgccgca aacgcggatt caggcacagg cgagcggtta ctggctggag
     6481 cgggagcagt ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac
     6541 cagcccgcgc cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag
     6601 gcccgcatcg agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca
     6661 ccgtgttatt tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc
     6721 ttctgtctgg aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat
     6781 ctcattgcgc tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg
     6841 ctgtaattta ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc
     6901 cgatcgagag aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata
     6961 ctgacattct tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag
     7021 taatggtgac caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga
     7081 cgggtgaaaa agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata
     7141 atcgctgcca gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca
     7201 aagcccatca ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca
     7261 attaataaca aaacggggcg ggcagctcca aaatcaaaat aacgtagtgt gttcataacc
     7321 aatccctttg caagataatg tgatttaaac gtaatacaca ctcttgcacc tcgccacgcc
     7381 ccaggcattg agatattcgc taattatcct ggttacagtg atttaagcgc gatgttctca
     7441 tctatcagca gtttcgcatc aaacgttttg ccgctctgga tccatttgcg aattgggcga
     7501 atctcacgcc cctgattcag cgtgaccgca ccgataagca cgccgttttg cagattaaac
     7561 caaatcgcct tctgagtttc cgggttgcca cgacaaagcc agtcatcgcc acgcatatcg
     7621 ccaataaact gtaagttatc actgtactga tcgctccaga accacggcgg cggcagtagc
     7681 ggtagcggta gccctaacat tgcggcagcg gcaatttgcg cctggttatt ggcgttttcc
     7741 cagctttcgc agcggtgtag tgcaccatta tcaagacgag tgattgccac atcgccaccg
     7801 gcaaagatcg cgggatcgca ggtgcggcaa gcctcatcaa tgacaatgcc attggcagta
     7861 tcaaggttgg cctcgcgagc cagttgctcg ttggcgctga taccaatacc gtaaatcacc
     7921 acatcagcct gaagcgtttc cccactttgc agcgtcagtt ctactttttc accatcgacc
     7981 acatgttcaa tggcattatt gagcagaatg cgcacaccag cctgctggtg gcgctgtaaa
     8041 agatagcgtt gcacgggcgg tggtgcatta cggcccatga cggttgccgc cagttcaatc
     8101 actgtcacct tacatctgcg ctgcgtggcg ctggcagcca gttccagacc aatagttccg
     8161 gcaccgataa tcacgactga ccgttcgggc tgcagaactt ctcgcagtct ggcggcatca
     8221 ccggcatggc gtagggtaaa gcagcgttct cccagtgcat caagcaacgg cagcggtcga
     8281 gctgccgcgc cggttgctat aaaaagctga tcccagtgcc agctttcgcc gttggttaac
     8341 actaactctc gtgtgtcgcg acccagcgtt ttgatggtta caccggaatg cagatgaaca
     8401 ttgttttcct gccaccagtt agcgggtaac acctgctgta actgcgggga atcttccagc
     8461 aacatggatt tcgagagtgg aggtcgttca taaggaagat gtcgctcatc ggaaaacaga
     8521 tgcagctcac cggtgaaccc ttgctggcgt agcgaggccg cagccattgc cgccgcttgc
     8581 ccgccaccga caataatgat cgttttttct ttcatcgtgc ttccttaaag atccagccca
     8641 gccgctacgt ggcgaatgcc gcgaatcgct aaacccgcat cagcgttgat catcacaccg
     8701 cttaatgcgc gattattgcg ccgcgatgcc aacatcacat acggccccgt aaaatccgcc
     8761 ggttgcggga aaaattgcag cggtaaaatg gcggcaattt tctccggcgt cagagactgc
     8821 attatcgagg tttcactttg cccgagcgcc tgtgggccgc gcaggtcgct ggccatacca
     8881 cacgggccga cgccattcac ccgcactttc ggtgccagtt cataagccag ttggcgaata
     8941 agtccggttg cggcatgttt actggcggtg tacagcgggc caccgccgcc aggataccag
     9001 gcggcatttg acagtgtgaa aatcatgctg ccttcactgg cgattaacgc cggagcgcag
     9061 gcttttgcgc ccagcaggta accgagtacg ttgacgttaa acagctcgtg gaagccggtt
     9121 tcgagcgtct ctgcgggagt attaaccagt gaggcattgt gatcccagat gcctgcattg
     9181 ccgataaaac aatccagctt gccggaacga gtcaggatct gatcgaccgc gcgttgataa
     9241 tcggcataac aggtcacgtt accttccacc gccagaatat gttcgccaaa tcgctgacgc
     9301 agactggcga cttttgccgc cgacagttcc agcgtggcaa cctgcgcgcc ttcttcgata
     9361 aatcgctcga ccagcgccag ccctaatccc gatccgccgc cggtaataaa aatggactcg
     9421 ttatgcagat cgctcatggc tgcgcctccg gtaagtcgat gaaaatgtca ccaccttcaa
     9481 cgtgtactgg ataagtggtg agcggatcgg tggcgggcag gcataacgct ttccccgttt
     9541 tcaggcaaaa actggcggcg tgtagcgggc actccaccgt ggcgtcatct tccagatacc
     9601 cttctgacat tgacgcatta ccatggctgc aacgatcgtt aatggcataa aactcgccgc
     9661 caacgttgaa cagggcgatg acgggcgagg tatcgatccg gagagcctca ccctccggca
     9721 catccgcaac gggacacgca taaattcgat tcatcagaac agtacactca ggttatggga
     9781 agtgattacc gcttgatcca ggacgatatc ccgttccagc aagcgccagt tgtcatcttc
     9841 cagacggcga actttgtcga aacgcgttcc aacatagaat gtttcatcgc gctctttttg
     9901 tgcccgataa agtagataat tcaccctcac ggcaaataca tttgggatgt cggtttcgct
     9961 tacctggcag ttgctgatta agtgacgagt gcgtgacggc ggctcttctg cccaggccat
    10021 gcccgtttcc agacgggcga ttcgccgctc cagctggtct ttggtgtcat taaaaatcca
    10081 ggtcgtcggt ggctgaacgc ctttgcggcg gtcgcgcgtt tgcgcgttaa ccgtggtgcg
    10141 catggtgtaa cgaatctctt cgtcgagctg tgccagccag tcacgaaatt tccagtcatc
    10201 cagcagactg gcttcgtgaa agagaaactg gctaatgcgg tgatgtaact ccagtgaaac
    10261 ttgcgcactc atttcatcac ctcctgctgg taagcggcgg ttttatcgag cacttcttgc
    10321 cagctttcgc tactcaggag atcggcccaa cgttggtaca ttccgcgagc ggcagtttct
    10381 gagaaaatat agttagtaat gccaggaatg ccgtcgtcgc gacgcttttc ctgaccaagc
    10441 cccatttcca gacacagttt gctgttgcgg gcgcggtggc ctttaagcaa tttctggatt
    10501 tcacaccagt tctccgagtc atcctgctcg agaaaaccag caggaccaaa agcacgagtg
    10561 gcgctgtttt caaaagcggc tttaacttca tcggaggcgg ctttgtcagt aatacagaac
    10621 gcccacactt caacttgatc agggccgcgc ggatgccaga cgcggagcgt ggcagtgccg
    10681 ttgagccatg aaagcgtggg gaaaatattg ttatgacccg ccaggcgcag ggcgcgaact
    10741 tcacctaaac gttgttctgc ttcggcatag gtttcgcggt aatagcttga aactgcgcca
    10801 tcgacccaga cattagcatc cggtttttca gtaaagaaga aaccgctacc gtgaccgtcc
    10861 tgaccaaatt gcagcgcatc tttggcggtt tcccacaccg ggcgggcggt ttgtccatca
    10921 ccgaggcgct tatcgctgcc atcatctttc gcccctaata cctgaacggc agaagcatgg
    10981 ctgaacagag catgatactg gtcactggcg aactgctctg ccgggaattt ccagttgcag
    11041 ttgatcaccc acttttgtac gccgccgaca atttcggtgc cgccttcgcg acgatccagc
    11101 atgccatcca gataccaggc aatgtcaccc aggtaatcac gcaggcccgg tgcgctggta
    11161 tcccagttgc caaaaatcag ccctttataa ctctccacac aaggaacttc gtttagcccc
    11221 cagtgggatt tacacaaccc ttgtggatag gcgcgaggtt ccagcggtac atcgatcaac
    11281 tcgccgttaa tgccataaga ccagccgtga tacgggcagg taaaggcgcg agtgttgccg
    11341 caatctgcat aactcacacg catggcccgg tggcggcatt ggttgagaaa cgccttgatg
    11401 ctgccgtctt tctgacgcac tacgacaacc gcatcttctc ccatgtaggt gttaaagaaa
    11461 tcaccgggtt ttgggatctg gctttcgtgg gcgagaaata gccagcaacg accgaaaata
    11521 cgctcaagct ccagttggta aatgtccggg tcggtataaa tacgcggagt gacccgaccg
    11581 ttttgggtgt caatcagttg gtaaatgttc aaatctgagg gtgtggtcat attttttaat
    11641 cctgaaagat aagatgtatt gaccttctat ttttgtgcca taaggatgtt gctggttggt
    11701 tgctaatatg tgaaatagtt aatttctacc agctgagacg ttttttcgat aacaagggga
    11761 aggtgatgga actacggcat ttacgctatt tcgtcgcagt ggcgcaggca ctgaacttta
    11821 cccgtgcggc ggaaaaactg catacctcac agccttcgtt aagcagccag atccgcgatc
    11881 ttgaaaactg tgtcggtgtt ccgttactgg tgagggataa gcgcaaagtc gcgttgacgg
    11941 cggcgggaga atgttttctc caggacgcgc tggcaatcct cgaacaagcg gaaaatgcca
    12001 aattacgggc gcggaaaatt gttcaggaag acagacaatt aaccattggc tttgtgccat
    12061 cggcggaagt gaatttattg ccaaaagtat taccgatgtt tcgtctcaga cagccagaca
    12121 ccttgattga gctggtgagt ttaatcacca cgcaacagga ggaaaaaatt cgccgtggtg
    12181 aactcgacgt cggcttaatg cgccatcctg tttatagccc agagattgat tatctggagc
    12241 tttttgacga accattagtg gtggtgttac cggttgatca ccctttagca catgaaaaag
    12301 agatcaccgc cgcgcaactg gatggcgtga atttcgtcag taccgatccg gcgtattccg
    12361 gttcgcttgc gccgatcgtt aaagcgtggt ttgcgcaaga aaacagccag ccaaatatcg
    12421 tccaggtggc aacgaatatt ctggtgacca tgaatctggt gggcatgggg ctgggcgtca
    12481 ctttgatacc cggttatatg aataatttta ataccgggca ggttgttttt cgtcccattg
    12541 ccggaaatgt gccttccatt gctttactga tggcgtggaa gaaaggggag atgaagccgg
    12601 cattacgcga tttcatcgcc attgtgcagg aacgtttggc aagcgtaacg gcataattac
    12661 gcattatttt tgcaacatgc gtcgcggatc tcagtttttt agtgcttgtg aactggcgtt
    12721 acagacatcg ggaagcaggc tcgaatattc ttatttttca acttgcacca acattcgatt
    12781 aaccccagac taacgcctcc tgacgggagg gactcatggt tttgcaatcc acgcgctggt
    12841 tggcgctcgg ctatttcaca tactttttta gttacggcat ttttctacct ttctggagcg
    12901 tctggcttaa agggattggt ttaacgccag aaaccatcgg cctgttattg ggggcaggtc
    12961 tggttgcccg ttttctcggg agtttgctca tcgcgccccg cgtcagcgat ccttcccgcc
    13021 tgattttcgc cttgcgcgtg ctggcactgc tgacacttct ctttgctgtc gccttctggg
    13081 cgggggcgca cgtagcgtgg ctgatgctgg tgatgattgg ctttaacctc tttttctcac
    13141 cgctggtacc gttgaccgat gcactggcga atacgtggca aaagcagttc ccgcttgatt
    13201 acggcaaagt gcgactgtgg ggctcggtgg cgtttgtcat tggctcggcg ctgacgggca
    13261 aactggtcac tatgtttgat tatcgggtga tcctcgcgct gttgacgttg ggcgtggcat
    13321 ccatgctgct cggctttctc atccgtccga cgattcagcc acaaggggca agccgccagc
    13381 aggagagcac cggttggtca gcgtggttgg cgctggttcg ccagaactgg cgctttctgg
    13441 cctgcgtttg tttattgcag ggggcacatg cggcctatta cggttttagc gccatttact
    13501 ggcaggcagc tggctactcg gcctcggcgg tggggtattt gtggtcgctg ggcgtggtgg
    13561 cggaagtcat tatctttgcg ctgagtaata aacttttccg ccgttgtagt gcacgcgata
    13621 tgctgttgat ctcggcgatt tgcggcgtag tgcgctgggg cattatggga gcaactacgg
    13681 cgttgccgtg gttgatagtg gtgcaaattc tgcattgcgg caccttcacg gtctgccacc
    13741 tggccgccat gcgttatatt gctgctcgcc agggtagcga agtcatccgt ttacaggcgg
    13801 tttactctgc cgtcgcgatg ggcggcagta tcgctatcat gaccgttttc gccggtttcc
    13861 tgtatcaata tctgggccac ggcgtgttct gggtaatggc gctggtggcg ctgccggcaa
    13921 tgtttttgcg cccgaaagtt gttccctcat gctgattcga gcattttgcg gatctgctcc
    13981 tgttgatgtg ttgtcagcgt caggtccgta tagatcagcg gtggtgaaaa gagcggtaat
    14041 ggcatggtat aaggcgcaat aatcagtgcc gcgccacgcg gagcgcctgt ctgcaaaaat
    14101 gcctttaccg acatatgctt aatgttgagc ggcagtaacg ttagttcgcg taactgctgc
    14161 tcaatctgcg cttctcgctc gctatcattc ccggtcagta gaataatctg tttttcatgc
    14221 agatcgtttt cctgcattag ccaggcaccg aaaatcaccg cgaccagacc actttcctca
    14281 tcagaaagat ggacgccgta ttcactttca aatccggcca gcgccgcgcg ggtggtgcgc
    14341 accaggcgtg ggtacagtct ggcgaactct tccggcaggg tattatcaat accgatggca
    14401 aacaaactgc gatttaacgc ctgggcgagg tgggtataaa gctgatcaca taacccctgt
    14461 tcatcataaa aacgaacatt tcccagctca cgaaaatggt ttaccagacg tttgatagac
    14521 tgacgcaatt gtctgtctcg ctgatgcgca tcccgcagtg gatcgggaac ccgcaacatg
    14581 gaaaaaagta gtgccataaa cagtggctca tcaggcggta caggctggag cgcccgacgc
    14641 tgccagtggc gtccaatttc ctgcgctacc tgaaattcaa ggcaggattc cgcccagcga
    14701 cgttggagcg gattaaactg aggcgtcatt ccagcgtggt gctgcagcaa acaatattgc
    14761 agatacagac acaggaaatg aatatcgcgc gattcaaagc gtttttgcag ccgtcgggag
    14821 cagagattca ctaacgcttg tagattggta tcgtcgtaaa agttacgcgc aattccgcgc
    14881 cgcttcaatt cactcttcag ggcgggggtg aattggctgg taataaatga cggacacaga
    14941 cggaaaccac gtcgtagcca gtgaaataaa cacaaacgtt ggttaagcac tgtaccttca
    15001 acccggtagc taccgtcata accagtcgtc agtgtgagtt gatgatagcg caggatctcc
    15061 cgtcctgttt cgctgatatc aagactggca atatcatcat ccacaccatt aagctcgctg
    15121 aaggttgcca tggtggcggt caaccccggc tgaaagagcg tcagcaagat ctggcagcgg
    15181 cgctggggag ccgaaaggac agatggtgga gcaagcgtag gcatcatctg tagatctccc
    15241 ataagcataa ttgataagaa tagctaaagt ttgctcaggg aaatgacgaa gggaatgcta
    15301 atcatcagaa atgttggcaa gcgtcacaga atttttctca tcttaaactg ttctgaccag
    15361 tggattagca tgtcttaagg tggttttgtg aatgattatc gtgctagcga tcatgggtta
    15421 gttctgcgga ttcaacgcgc taaggataaa gtccaccatc tgttcgcgat aaacatcgcc
    15481 gtgacgatcg gaaaaagcat caatgtgttc gccatccggg attaggatca accgttttgg
    15541 ctcttttgcc aggctataca acttttcgct gtgctgccat gggataacgt gatcggcttt
    15601 accgtgaatg agtaaaagcg ggatcgggct gacgctggcg atgtaatttt cgccgctgta
    15661 actctcatca agtaagtagc cactgccggg gatcatctgg ttggcgatgg ttgcataaga
    15721 ggcaaatgtg gagtcgagga tcaccgcacg tatgccttca cgatcaccct gaccaataac
    15781 agcaagagta ttcgccccgc caatactctg cccgaacagc accagccgtt gtgggtttac
    15841 atcactgcga tggcgcacca cattgatggc gctttgcgta tcgtccagca atccggcctg
    15901 ggacggcgtg ccttttgatt taccaaaccc gtgataatca aacataaaaa cgttgaaatt
    15961 acgctcgggt aaccaactga ccagcggcca gtgggcggac atatttccgg cattgccgtg
    16021 agcatgaatg atggttgcga tggcgttgtc agcagggccc gtcgaagaag ggataaacca
    16081 gccttgcaga cgagtaccgt ccttagccgt aaattcgacg gattccgccg accaggggtc
    16141 gggaccgtaa attttatcgt caggataata gaagaagttg atggcaacgc gcggcactaa
    16201 ataaacgcag aaggcaacaa caaagagaat aaacagaatt ttgggaacgc gtttgttcac
    16261 tggcagtgcc ataacctttc cttgcgactt ctttatcatc cattaatcgg ggaaagatgg
    16321 tgggagagtg acgaaagaaa gtaaagcggt gaggaagtat ttcggaatag cgcctgaaag
    16381 gcgagggcgg gtgagtgata tcacccgcct gagtcattaa cgcttcagag cgtcgcttaa
    16441 ctcgtcacgc atgttcgcca gcatggcttt aacaacacgc gggttaccag caacgatgtt
    16501 accggtcagc atatagttat gaccaccggt gaagtcgctg acgatgccgc ccgcttcacg
    16561 aaccagcagc tcgcctgcag cgaagtccca cgggcgcaga ccgatttcaa agaaaccgtc
    16621 aacacgaccc gcagcgacgt aagccagatc cagcgccgca gaaccggtac gacggaagtc
    16681 tgcacattcg ttgaacagtt tgccgacgat gttgatgtag gtagtggcgt actgttttgc
    16741 tttgaacggg aagccggtcg ccagaatagt accgtcgaga tcgcgagcgg tgctgccgcg
    16801 cagacggtag ccgtttagct gtgcgccctg accgcgagtg gcggtgaaca gttcgttacg
    16861 cataggatcg tataccacag caacttcggt gcggcctttg atacgaacgg cgatagatac
    16921 cgcgaagtgc ggcagacgtt tgataaagtt ggtagtgcca tccagtggat cgataaccca
    16981 ttgaacatcc tgatcagtac cttcaagttc accgctttct tcggtgatga tggtgtgctg
    17041 tgggtaagat ttacgaatcg tgtcgataat caccgcttcg gcagctttat ctacgttggt
    17101 cacgaaatcg ttactgcctt tctggctcgc ttctacagcg tccggggttt catagttttt
    17161 ggcaattaaa ttacccgcct tgcgcgctgc gcgcacggcg atgttcagca tcggatgcat
    17221 cggtctctct cactggatgt taaagaacgg gaaaacggcg cagagtatag cagcgagatc
    17281 ggcatctgtc ttcgttttat gataagatgc gtgaataatc tttagatgaa gaaagacaat
    17341 gctgcaaaat attcgaattg tgctggtgga gacgtcacac accggcaata tgggttctgt
    17401 tgcccgtgcc atgaaaacaa tgggattaac caatctgtgg ctggttaatc cactggtgaa
    17461 acccgactcc caggcgattg ccctggcagc aggggccagc gatgtgattg gtaatgctca
    17521 catcgtcgat acgctcgacg aagcgttagc cggttgtagc ctggtggtgg gcaccagcgc
    17581 acgctcccgc acgctgccgt ggccgatgct cgacccgcgc gaatgcggtc tgaaaagcgt
    17641 cgctgaagcg gcaaataccc cggtggcgct ggtgtttggt cgtgagcgcg tcggcttgac
    17701 caatgaagag ttgcagaaat gccattatca tgtcgcgatt gcggctaacc cggaatacag
    17761 ctcgctgaac ctggcgatgg cggttcaggt tattgcctat gaagtgcgca tggcctggct
    17821 ggcaactcag gaaaatggcg agcaggtcga gcatgaagag acgccgtatc cgctggtcga
    17881 tgatctggag cgtttttatg gtcatctgga acaaacgctg ctggcaaccg gttttatccg
    17941 tgaaaaccat ccggggcagg tgatgaataa attgcgccgt ctgtttaccc gtgcgcgccc
    18001 ggaaagccag gaattgaata tcctgcgcgg gattctggct tctattgagc agcagaataa
    18061 aggtaacaag gccgaataac agtggttgcc tgatgcgacg cttaacgcgt cttatcaggc
    18121 ctacaatgta cagaaccgta ggtcggataa ggcgttcacg ccgcatccga cagccgttgc
    18181 ctgatgcgac gcgtaatgcg tcttatcagg cctacagtgt acagaacccc agggcggata
    18241 tggcgttcac gccgcatccg acaacaggta caaacgccac gataaaaaaa tggcactgaa
    18301 ggttaaatac ccgactaaat cagtcaagta aatagttgac caatttactc gggaatgtca
    18361 gacttgaccc tgctatgcaa tacccccact tttacaataa aaaaccccgg gcaggggcga
    18421 gtttgaggtg aagtaagaca tgagactgac atctaaaggg cgctatgccg tgaccgcaat
    18481 gcttgacgtt gcgctcaact ctgaagcggg cccggtaccg ttggctgata tttccgaacg
    18541 tcagggaatt tccctttctt atctggaaca actgttttcc cgtctgcgta aaaatggtct
    18601 ggtttccagc gtacgtggac caggcggtgg ttatctgtta ggcaaagatg ccagcagcat
    18661 cgccgttggc gaagtaatta gcgccgttga cgaatctgta gatgccaccc gttgtcaggg
    18721 taaaggcggc tgccagggcg gcgataaatg cctgacccac gcgctgtggc gtgatttgag
    18781 cgaccgtctc accggttttc tcaacaacat tactttaggc gaactggtta ataaccagga
    18841 agtgctggat gtgtctggtc gtcagcatac tcacgacgcg ccacgcaccc gcatacaaga
    18901 cgcgatcgac gttaagttac gcgcttaatt taaccgtcct gtttcgtgtt gcatctgcgt
    18961 tgactgcaca tactcactcc ggtcacttac ttatgtaagc tcccggagat tcgtaagttt
    19021 gtcaacttgc tgaaacccga aacagttagg ttaaagataa caagatatta gaatcaggcc
    19081 ggggcgtatc gcgctccgta aacacggtcg tacatccagc cggtagcctg attccttgca
    19141 ttgagtgatg tacggagttt atagagcaat gaaattaccg atttatctcg actactccgc
    19201 aaccacgccg gtggacccgc gtgttgccga gaaaatgatg cagtttatga cgatggacgg
    19261 aacctttggt aacccggcct cccgttctca ccgtttcggc tggcaggctg aagaagcggt
    19321 agatatcgcc cgtaatcaga ttgccgatct ggtcggcgct gatccgcgtg aaatcgtctt
    19381 tacctctggt gcaaccgaat ctgacaacct ggcgatcaaa ggtgcagcca acttttatca
    19441 gaaaaaaggc aagcacatca tcaccagcaa aactgaacac aaagcggtac tggatacctg
    19501 ccgtcagctg gagcgcgaag gttttgaagt cacctacctg gcaccgcagc gtaacggcat
    19561 tatcgacctg aaagaacttg aagcagcgat gcgtgacgac accatcctcg tttctatcat
    19621 gcacgtgaat aacgaaatcg gcgtggtaca ggatatcgcg gctatcggcg aaatgtgccg
    19681 tgctcgtggc attatctatc acgttgatgc aacccagagc gtgggcaaac tgcctatcga
    19741 tctgagccag ttgaaagttg acctgatgtc tttctccggt cacaaaatct atggcccgaa
    19801 aggtattggt gcgctgtatg tgcgtcgtaa accgcgcgta cgcatcgaag cgcaaatgca
    19861 cggcggcggt cacgaacgcg gtatgcgttc cggcactctg cctgttcacc agatcgtcgg
    19921 aatgggcgaa gcctatcgca tcgcaaaaga agagatggcg accgagatgg aacgtctgcg
    19981 cggactgcgt aaccgtctgt ggaacggcat caaagatatc gaagaagttt acctgaacgg
    20041 tgacctggaa cacggtgcgc cgaacattct caacgtcagc ttcaactacg ttgaaggtga
    20101 gtcgctgatt atggcgctga aagacctcgc ggtttcttcc ggttccgcct gtacgtcag
//
LOCUS       DACAPB010000073        19839 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.073, whole genome shotgun sequence.
ACCESSION   DACAPB010000073 DACAPB010000000
VERSION     DACAPB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.073"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..304
                     /locus_tag="IT904_002370"
     CDS             <1..304
                     /locus_tag="IT904_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAP3192141.1"
                     /translation="LTTRLAEGLLEVAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQD
                     VMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(462..2444)
                     /gene="fhuB"
                     /locus_tag="IT904_002371"
     CDS             complement(462..2444)
                     /gene="fhuB"
                     /locus_tag="IT904_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAP3192142.1"
                     /translation="MSKRIALFPALLLALLMIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLALALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSL"
     gene            complement(2441..3331)
                     /gene="fhuD"
                     /locus_tag="IT904_002372"
     CDS             complement(2441..3331)
                     /gene="fhuD"
                     /locus_tag="IT904_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAP3192143.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(3331..4128)
                     /gene="fhuC"
                     /locus_tag="IT904_002373"
     CDS             complement(3331..4128)
                     /gene="fhuC"
                     /locus_tag="IT904_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAP3192144.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(4179..6422)
                     /gene="fhuA"
                     /locus_tag="IT904_002374"
     CDS             complement(4179..6422)
                     /gene="fhuA"
                     /locus_tag="IT904_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAP3192145.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALYVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(6642..9176)
                     /gene="mrcB"
                     /locus_tag="IT904_002375"
     CDS             complement(6642..9176)
                     /gene="mrcB"
                     /locus_tag="IT904_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAP3192146.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSSNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(9372..11801)
                     /gene="hrpB"
                     /locus_tag="IT904_002376"
     CDS             complement(9372..11801)
                     /gene="hrpB"
                     /locus_tag="IT904_002376"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAP3192147.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LTGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPTQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDNESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            11875..12405
                     /gene="thpR"
                     /locus_tag="IT904_002377"
     CDS             11875..12405
                     /gene="thpR"
                     /locus_tag="IT904_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAP3192148.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGDVSAEKEKALSLLAGRIRQSGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            12420..13124
                     /gene="sfsA"
                     /locus_tag="IT904_002378"
     CDS             12420..13124
                     /gene="sfsA"
                     /locus_tag="IT904_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP3192149.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            13302..13757
                     /locus_tag="IT904_002379"
     CDS             13302..13757
                     /locus_tag="IT904_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAP3192150.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            13794..14720
                     /gene="gluQRS"
                     /locus_tag="IT904_002380"
     CDS             13794..14720
                     /gene="gluQRS"
                     /locus_tag="IT904_002380"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414686.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAP3192151.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKSDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            14759..16177
                     /gene="pcnB"
                     /locus_tag="IT904_002381"
     CDS             14759..16177
                     /gene="pcnB"
                     /locus_tag="IT904_002381"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAP3192152.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNISDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            16174..16653
                     /gene="folK"
                     /locus_tag="IT904_002382"
     CDS             16174..16653
                     /gene="folK"
                     /locus_tag="IT904_002382"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAP3192153.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLQNRLTILNTSQPVIW"
     gene            17003..17596
                     /locus_tag="IT904_002383"
     CDS             17003..17596
                     /locus_tag="IT904_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192154.1"
                     /translation="MKKTLLAAALVMVSGSALAVDGGHIDFNGLVQSGTCKVGVVDAG
                     MHGITTDGVVTLDTANVTDNFAEVNATAVGLLPKEFMISVNCEPGKTEASLTMGSASY
                     ANTSGTLNNNMNITVNGIQPAENVNIAVHNMTNKSGAAEIKQVHMNNSAEKHLLTLDA
                     NGDGQYVFNASYVKAPNSQDVTSGHVTTNALYTITYN"
     gene            17679..18404
                     /locus_tag="IT904_002384"
     CDS             17679..18404
                     /locus_tag="IT904_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAP3192155.1"
                     /translation="MFPIIKKTVSALFISTLLTSAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTSTPPVSRIESKRGQTVKVMFTGTT
                     QLPADRESVFWFNVLEVPPKPKDAESDKNLLQLAFRTRIKLFYRPSGLKGEPAEAPAK
                     LTWKLNNAQLQVNNPTPYYVSFNDVAIESGGKSYKVDSSMVAPFGQVSFAVTDIASSV
                     SSGKVIYKAINDYGGNIDGSTSL"
     gene            18445..>19839
                     /locus_tag="IT904_002385"
     CDS             18445..>19839
                     /locus_tag="IT904_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HAP3192156.1"
                     /translation="MYTLTHQKSRLPKTTLLAACCAFFYSSNGAATESVEYDSSFLMG
                     TGASSIDVKRYSQGNPTPPGVYNVRVFINQQSVASLELPFVDIGENSAAACITLKNLA
                     QLHIKKPEYPITLLAREGEEGDCLDIKKSIEQAEVSYDGSEQHLEISVPQAYVYKTYG
                     GYVDPSLWESGINAAMLSYSLNAYHSDSKNGNRDSIYGAFNTGLNLGAWHLRARGNYN
                     WSQDNGNSFDFQDRYLQRDIPAIRSQVIVGDAYTTGETFDSVNVRGMRLYSDSRMLPS
                     ALASYAPIIRGVANSNAKVTVTQNGYKIYESTVPPGEFVIDDLSPSGFGSELVITVEE
                     ADGSKRSFTQPFSSVVQLQRPGVGRWDISAGQIIDDSLRHEPNMAQASFYYGFNNLFT
                     GYTGVQITDNDYMSGLLGLGINTSIGAFAVDITHARTEIPDDKTYQGQSYRITWNKLI
                     EATSTSFNLAAYRYS"
BASE COUNT         4872 a         5494 c         4953 g         4520 t
ORIGIN      
        1 gctgacaaca cgtctggcag aaggtctgct ggaagtggca gaagaagccg gaattccgct
       61 ggtcgttaac cacgttggcg gcatgttcgg tattttcttt accgacgccg agtccgtgac
      121 gtgctatcag gatgtgatgg cctgtgacgt ggaacgcttt aagcgtttct tccatatgat
      181 gctggacgaa ggtgtttacc tggcaccgtc agcgtttgaa gcgggcttta tgtccgtggc
      241 gcacagcatg gaagatatca ataacaccat cgatgctgca cgtcgggtgt ttgcgaagtt
      301 gtgatccagc atggtttgtc ggatgcggcg tgaacgcctt atccgaccta cgattcgggg
      361 ccgtttgtag gcccgataag acgcgccagc gtcgcatcgg gcatccgttg tcgcgtaaac
      421 gctttatccg tccaggcgta cagggccgtt atatggaaaa attaaaggct ctgctttctc
      481 agcaaataga taaaatatgg cgcgccgata aaggtcgaca gcagccccgc cgggatctgg
      541 aacggaaaca gcaccatccg cccacaccag tcagcgaaca ccagcagtaa accacccacc
      601 agcgccgaaa ttacgatgtg tggcatcgtc cgtcgaaaac ccatcatccg cgcaatatgc
      661 ggtgccatta aaccaacaaa actcaacggt ccaatagtca tcgtcgcggt cgccgtcagg
      721 caagccgcta acagcagcag cgcaattcgc gtcggcgtca gcgccattcc tacggctcgg
      781 gcggtatcac cacccagcgg taaaatggtc agccagcggc ggcacagcgg ggtaatcgcc
      841 agcaaaatca ccatcacaat tccggtgcgc cagacctgcg catcggtcgc gttgtaggtc
      901 gaaccggaaa tccaggtcag cacttgcgcc attcgcgggt caccacttgc ctgcaacatc
      961 atcaaaagca tggtgaacgc ggtgcttaac gccatccccg ccagtaacat acggtgtggg
     1021 gaaaatccac cgcggccggc ggcgatcata atgatcaaca gcgtcaccgc cgcgccgaga
     1081 ctgcctgcag gtaacagcca gccaaaggca ttacccggca ccagaaacag catcaacacc
     1141 acgccaaacg ccgcgccgga gctaatcccc agcacttccg ggcttgccat cgggtttccg
     1201 gtcagtcgct gaataataca gcccgccacc gccagcatga cgcccgcaaa cagcgccgcc
     1261 ataattcgcg gccagcgcca gggcattaaa tcctcgagca acgccccgct cgcccacgtc
     1321 cagccgtgcg catcacgacc aaacgacagc gccaccacca cagccatcaa cagcagcacg
     1381 ccgcccgcga gggcaagcgc cagcacatgt tggcgttcag ccgcgacacg atcgttgacc
     1441 ttcatatccg gcgcgctaat gctgcgtaaa cgcggcaaca gccacagtag cagcggcgca
     1501 ccgatcaacg cagtgaccga accggtggaa acttccatcc acacgcgagt cagccagagg
     1561 atgatttgat cagaaagcca cagaattaac gcaccaatca gcgacgccag catcaaccgt
     1621 ggcaacaaac gccgcgcccc cagcattttt gccagcagtg gcgcgaacaa cccgataaag
     1681 ccgataatcc ccactgcgtt caccagcagc gcactgatga ctatcgccag cgacagcgcc
     1741 gccagacgcg cgagcgacaa ggccagcccg agattgcgcg ccacgccatc atcaagcccc
     1801 atcagggtta acggacgaag tagcagtaac gtcagcatca cgccgcccag caactgcggc
     1861 cataaacgct caacgccgcc ccagtcggtt tgcgtcagcg ttccggtgct ccacaggaac
     1921 atgctttgca actggtcatg atggaagata accagtaact gattgattgc cccacaataa
     1981 aggctcacta ccagccccgc gagaatcagc gttaccggcg acagccgttt cccccacgcg
     2041 acgccaaaga caattaagcc aacaacgcaa gccccagcca gcgcagcaaa ctggctcgcc
     2101 atagcacctg ggatcgccca gagcgtagtg acggtaatcc ccagttgcgc gcctgttgca
     2161 acgcccaacg tcgtcggttc cgccagaggg ttacgcagca cttgctgaaa cagcacgccc
     2221 accagcccca ggcccgcgcc caccagcagc gaaatcgcca gacgcggcaa caagctgtag
     2281 tgaaaaatca tctgctcgat gacgtcaata tccggcgacc aggcagcctg cgcccactgg
     2341 ctacgcggca gcgcctgcga gaagttcatc caggtcagcg ccgtagcggc aatcatcagc
     2401 aacgccagca ataacgccgg aaaaagcgca attcgtttac tcacgcttta cctccgatgg
     2461 cgttatccag aacgcgcaca aagtgcattg ccgagagcgt cgcaccataa aaccagactg
     2521 cgggtacgcg ctgaaagcgt ccggcgcgga caaacggcat ggcctgccac agcggggttg
     2581 ccattagcgc gtccatgtct ttgctgttgt cgtgatcaaa acagagcaca tcaacatctt
     2641 tatacgccgc cagacgatcg atgctgacgg cggtactgcc ccagaagttg gtttcccctt
     2701 gccaggcatt tgggatgccg tactcatcaa gaatttcctg aaacaagctg tttggaccga
     2761 aaaccagcat atggcgcgga tcgataagcg tcgtcagcaa taacgggcgt tcaccacgct
     2821 tcacaaagcg gggtttcatg ctgcggataa agtcttcata gtgcgctaaa tgcgtttctg
     2881 ctgcgctttg caggttaagt aaatctgcca tttccgtcag tgacttacgc gccatcgcca
     2941 gcggctgttt gccatcgctg aagttaaatc cacgccccgg cgcaatacga gccagcattt
     3001 ctggtgaagg gccatatcct gccgaccaga ccataaacga tggtttcatt tcggtcagca
     3061 gttcaaggtt aggttctgtg cgcaaaccga cgtcgatcac tgagtccggc aatggtggtt
     3121 cgctgaccca caggcgatag ttgatggtat ccgccacgcc gtaaggcacg atgccgagcg
     3181 ccagcagtaa ttccaccggc aaccactcca gcgccacaat acgattggga tcaatagccg
     3241 ccgcgtgggc ggtattcatc tgccataaca acggggaaag cgccatcgcc gttaacagtc
     3301 gacggcgcga aataagaggt aagccgctca tcaataaaca aaactcacag gtgcagcacc
     3361 cgccggatgc ggcaaaatac ccatcgggat gccataaatc atttcgaggg tttcgccgcg
     3421 cataatttcc gcaggcgttc cctgagcaat catttcaccg ccgcgcaggg cgaccagata
     3481 atcacagtag cgtgccgcca tattgatatc gtgcaacacg gcaatgaccg tcaggccacg
     3541 ctcctgactt aaacggtgca ccagcgacag cacatcaacc tggtgggcga tatccagcgc
     3601 cgaggtcggt tcgtcgagca acagacaacg gctatcctgc gccaccagca tggcgatcca
     3661 cgcccgctga cgttcgccgc cagagagact atcgaccagc cgatgcgcca gcggttttaa
     3721 gccaaccagc gagatagctt cctcgacttt ttcgcgatct gccgccccaa agcgccccag
     3781 cgcgccatgc cacgggtaac gaccaatcgc caccagttca cgcacggtca tcccttctgc
     3841 cggaggaagc tgctgcggca aataagccac tttgcgggca aacgctttgc tgctccagct
     3901 ttccagcggt tgggcatcaa gaagaatctc cccttccgac ggcggctgat gacggccaag
     3961 cattttgagc agagtggatt taccagaacc gttgtgacca atcagaccgg tcactttccc
     4021 ggcaggaaag gttaacgaca gcggatgcaa aagcgtgcgc ccgggcacac gaaaggagat
     4081 attacgcagt gcaaaagtgg tatcggaatg attcgtgtat tcctgcataa cagccaactt
     4141 gtgaaatggg cacggaaatc cgtgccccaa aagagaaatt agaaacggaa ggttgcggtt
     4201 gcaacgacct gacgttctgc gccccagaag cagccataag tgttaaagca gctggcgacg
     4261 tattcacgat cgaacaggtt gttaacatac agcgccacgt tggagccagc catgccgact
     4321 cgcgccagat cataacgtac taacgcatcc acgaccgtat aacttcccac tttaaaggag
     4381 ttagccggat caccataact ggagccagta taacgaccac cggtgcccag cgtcagacct
     4441 gaaagcggac cgtcaaagaa ggtgtagtca gcccacaacg aagccatgtg ttttggcacc
     4501 tgtgcaggcg tattgccttt ataggtagta tcggtggtgt attccgcatc ggtgtaagta
     4561 taagaaccga ctacgttaac actcgccgac agcgccgctt tcgcttcgat ttctacgcca
     4621 cgagcgcgga tctcgccacc ttcaaccgag aagaaggaac cctcagggtc cgccatcagg
     4681 ttgtttgttt tagtgagatt atacacggca ccagtaacta caatcggacg atcttccggt
     4741 acatatttca cgccgacttc atactgctta cctttagacg gtgcgaaaat attaccatcc
     4801 ttcccaactt gcgaagaagg ttcaaacgat tcgctatagc tgaagtaagg tgttacacca
     4861 ttatcaaaca ggtagttaac accaccacgc caggtaaact gtttgtcatc acgtttatcg
     4921 gtcgtcccgg caacgcggtt aagagattct tgatctgccc agtcataacg accgcccagg
     4981 gtgaccagca ctttatccca ctgcgcctga tcctgaacat aaacgcccgt ttgtttctgc
     5041 ttattcagaa tgcggtaagg gccggagttt gccggatctt tggcattgaa gtcgaaatcg
     5101 gtattcaccg gattgtacag attgagcagt ggcacagagt cgtcgtaacc aaaccaggcg
     5161 ttgatgtcat tacgcatacg cataaagtcg acaccggtca gcagggtgtg gtcgatatcg
     5221 ccagtggcaa acttgctctg caactgggta tcaacggaga agttttgcag cttctcatca
     5281 tcaacgacgt atttacgtgc cagataatgg cctttatccg ctggcgctaa tgccgcacac
     5341 tgtttgctgt aagcattcgc cggatcggag cagacgccgt aaccataaac gctgttttgc
     5401 gaggttttgt tttcagcaaa gcgcaggttc tgacgcacag taaaggtgtc gttaaattcg
     5461 tgatcgaagc tgtagccgac catcttctca ttacgagaat aggtgttgtt cttcgcccct
     5521 tcattaaagt ctgtcggcag acgcttaccg ttcggcagcg gctcaacggt tccctctttc
     5581 ggcaaccagc cgtaataacc ggtttccggc tcgttctgga agtaagaaag gaaggtgaaa
     5641 ttggttttat catccggacg ccaggtgaac gccggtgcaa tagcataacg ctgctcttct
     5701 gaccctttct gctgggcatt ggcagaacgc gcaagaccgg tcaggcgata agagtaaacg
     5761 ccgtcatcat ccagcgcatc gctaaagtca aaaccagtct ggaacaggct gtcagtaccg
     5821 gctttaaact gaacttcttt gagcggttcg gtggtcggac gcttgctgac catattcaac
     5881 agaccgccag gactgctttt accgtaaagc acggaaaccg ggccacgcat aatttcagcg
     5941 cgttccagca tatacggatc aatgaccgca tcgttataga agttgccctg caacttcagg
     6001 ccattcagat agttattctg gctttggcct tctgccgcaa aaccgcgaat gatcaggtgg
     6061 tcataggtgt tggatgcgcc acgcgtacca acagagacac ccggcgtgta gctaagcgct
     6121 tcttttaccg acttcggctg atgcagcgcc atctcttcgg cggtcacaac agaaatagac
     6181 tgtggcactt tttgaatcgg cgtatcggtt ttagtgccgg tagctgactg tcgcgccgca
     6241 atagttgcag caggccccca tgcgctttct tgcggcgcag gtgcagcggt aacggtgata
     6301 gtgtcttctt tcggttcaac cgctgcctgt gcataaacag acatgccgct aaccgctgtg
     6361 gctactacaa ctgcgatttt acgcagtgag tgttttggct gagcagtttt ggaacgcgcc
     6421 attggtatat ctctgatgta aagtgaatga taacgtaaac gagaatgatt attataaaga
     6481 taagataata ttcgaaacgc tggcgggttc gcaagcggta cgcaatctga gcgttcctga
     6541 agggttaata aacaaccaga tgaaaagaaa gggttaatac tttgggtggg atgttatttt
     6601 accggatggc aactcgccat ccggtatttc acgcttagac gttaattact accaaacata
     6661 tctttaatcc aaccggctac accgtcgctg tctttctgct cttgctgagc aggttgctgt
     6721 tgcggctgtt gctgcggctg agaagactga tcaaacggat tgcttgacgg ctgctgctgc
     6781 atctcactct gctggcacag cgattgcgga tcgctggtcc agaccggcaa gacacgcatg
     6841 ccaccgctgc aaacaaagtt gccgtcgtag tccacgccca tatctgcaat atcttctggc
     6901 ggaacaagat tcagcggtgt tggcgtctgg ttagccagat aacgctgata aatcgacatt
     6961 gccccgctgg ctccatacag tttggtcggc tggttgttat cacggcctac ccaggtgatg
     7021 gtcaccgtgc tgccgtcaat gcccgcaaac caggtatcta cgttattgtt agtagtccct
     7081 gttttccctg ccagatgcag gttcgggtat ttcgccccaa gctgacgacc cgtaccgcgt
     7141 tgtacaacct gctgcatggt ccatagtgtc agatacgccg cctgcgccgg aacagcgcgt
     7201 tccgcctgcg ggaagctctg atacagcact ttgccatctt ccgcgattac cgaacgcagc
     7261 gcagaaagcg gtgcacggtt accaccgctg gcgatggtct ggaatgcctg cgccacttcg
     7321 attggcgtta agttcaacgc ccccagcagc atcgccggaa ccggatgcag ctgatctttc
     7381 ggcacgccca gtttgatcca ggtctccgta accgcaggca gccccagtgc catccccaga
     7441 tttaccgtcg gcacgttcat cgaacgggtc aacgcatcca ccagcatcac tctgccactt
     7501 tcgctataac gacgatcatc gttctgcggt gaccagacct ggccattcgg ctgacgcagc
     7561 gcgattggcg catccgcaat ccacgtgttc aggcgataga ttttcggctg gcttaaggca
     7621 gtcagataag tcgctggttt tgcaagggaa ccaatcgaac gacgcgcctg catcgcacgg
     7681 ttgtagcccg caaactgcgg ctcagaacct ccgaccatcg cacgaacttc accactaaag
     7741 cggtcgacga ccacaatcgc ggtttccaga tcgctcagct tacgctgttt cttcagtgcc
     7801 ggaatgcctt ccacggcggc tttttctgcc gcgtcctggg ccaccgagtc aaaggtagtg
     7861 aagatcttca cgccggacag atcttttacc ttatcgccta atttcgcctg caactcctga
     7921 cgcaccagtt gcataaaggc tggctgtgga gagattaccc caccgcgcgg ctgtacgccc
     7981 agaggacgcg cgctgagcat ttcatacagt tcaccatcga taatctgctg ttgttgtagc
     8041 aaacgcagca ccagattacg tcgctccagc gccagtttcg ggttacgcca cgggttgtag
     8101 atggacgccc ctttcaccat gccaactaac agcgcctgct ggtcaaggct caactcttct
     8161 accgggcgac caaagtagta caggctcgcc agcgggaagc cacgaatttc gttgtcgccg
     8221 ctctgaccga gatacacctc attcatatac agctcaagaa tgcgatcttt gctgtaacgc
     8281 gcatccatga tcaacgccat ataggcttcg ttcgctttac gccagtaaga gcgctcgctg
     8341 gagaggaaca ggtttttcac cagctgttgc gtcagcgtac tcgcaccctg caccgtgcga
     8401 cctgcggtca ggtttgccag caccgcacgt ccgatggagt agaggctgat gccgtcatgc
     8461 tcgtaaaaat gacggtcttc tgtcgccagc aaagtatcca ccagcaaatc agggaaacca
     8521 ctgcgcggca caaacagacg ttgttcaccg tttggcgagg agatcatggt gatcaggcgc
     8581 ggatcaagac ggaagaagcc gaactgacgg ttgttctcca tattgacgat ggtcgccaga
     8641 tgatcgccat caaaggtcaa acgcgcacgc acctgccctt ctttgctgtc cgggaaatca
     8701 aacggacggc ggatcatctc aatgctgtta gcctgcacgg taaattcgcc aggacgggtc
     8761 attttcgaca cctgacgata ctgggtcgcc tccagcagct tcaccatctc gttcttgctg
     8821 atggtcatgt ctggctcaag attgaccatt cggccataaa ctgccgcagg cagttgccag
     8881 accttgccat caatacggct acgaattttt tgatcgagat aaacgccgta aatggcgatc
     8941 agcacggcaa aaacgatagc cagttttagc agtagccata gccagccgcg tttgccacga
     9001 ggcttacgcc ctttgccttt gcccttacct ttgcgcggca tcggttcttc atcctcatag
     9061 tcatcataat cgtcgtaatc atcgtcatct tcgtaacgac gacggcttac cttttgtttg
     9121 accggacgcg tcggtttccc tttgcgtcca attggctcgc ggtcattccc ggccatgctt
     9181 tttctccgca atattcaggc gcaaaagccc gattttctgt tcttctgtta caagacagaa
     9241 gaagatatct ctcaaaattt gtgctcgatc ggtcccctct cccttgaggg agagggttag
     9301 ggtgagggtg aacgtgcgcg ctgatgccct caccccggcc ctctcccaca gggagaggga
     9361 gaaaaccaac cttacgaata ctttttcgtc cgtcgcgtcg gtgcagtgtt tgccgggtcg
     9421 tccggccaga catgtttggg ataacgccct ttcatctctt tttgcacctc acggtacgct
     9481 cctttccaga aggcgctcaa atctcgtgtg atttgtaatg gcctttgggc aggggaaagc
     9541 aactccagca ccagcggcac gcgcccctgg gcgatcgtcg gattggtggc ctcaccaaac
     9601 atctcctgca ttctcaccgc cagcgcgggc gggttatctt catgataacg aatggcgatc
     9661 cggcttcccg tcggcacagt gtaatgcgca ggcaattcac tatccagacg ttgctgcatt
     9721 ccccaatcaa gtaatccgcg tagtgcctga taaatgtcga gtgatttcag gccgcgtagt
     9781 gaatgcacgc cggtcatatg tggcagcagc cacgtttcca gcgtcgccaa caaactttca
     9841 ttatcaaccg caggccagtc atattccggc aaccactttg cggcgcataa caaacgcaag
     9901 cgtagctgtt ccgcttccgc cgtccagttg agcacgctta aacctttatc acggatgcca
     9961 ttaagcatcg cctgatgcaa ctcgtcttct gacggtttcg ccaacggctg cactttcacc
    10021 gtcaactgac cgatttgcag ccgacgccag gctttcagcg taccttgcgc gtcatcccat
    10081 tccacggtgt cagattgctg caccagctgc ggacagcgtc gcactaattc atcaatatcg
    10141 accggtagcg ccagtaaaat ccgcgcatcc ggcgaggcgc tgccctgcaa taataacggt
    10201 gcgatcaacc attcgtggcg gcttagcgcg tcgtcggcat cgagcatcgc tcccatgccg
    10261 tttgccagtt gatagcgtcc atcttgccca cggcggcgag caatgcgatc ggcaaacgcc
    10321 ccggcaagta gcggcgcgat aagcgaactg tctgcctcac cgccgcgtac gtttaagcgt
    10381 tttaacagtt gctgactacg ttgctgccag gctggttgat tgcgcgaaaa cgccacgccc
    10441 aggtcactat tgcccatccg tggcggctct tcgagaatgg cggcaatttt tgccgcggta
    10501 gcagcttcgt cgtcgttctt cgcgctaacc agcattgccg ctaaacgcgg atcgttaccc
    10561 agcgctgcca ttttttgccc ttgcgcacta agccgttcac cgtccagcgc ccccagcatc
    10621 cgtaacaggc gtttcgcggc cagtagattc accgttggcg gttgatccag ccagctcatc
    10681 tgcgtcggat cgctgcatcc ccattgcagt aattccatca gcaaaccgga aagatcgctt
    10741 tgtaatattt ccggttcact ttgcgccgcg gcgcgttctg cttgttcctt ggcgattaaa
    10801 tgcaggcaga tacccggctc cagacgcccg gcgcgcccgg cacgctgcgt catggatgcc
    10861 tggctaacgc gttgagtaat cagccgcgta agccccgtgc gcggatcaaa gcgcgccaca
    10921 cgctcctggg cacaatccac caccagacga ataccttcga tggttaaact ggtttcagca
    10981 atattggtcg ccagcaccac tttgcgcatc ccttgcggtg ccgggaggat cgcttttcgc
    11041 tgatcgttca gcgacaacgc gccatacagc gggcagagca acacatcact gccgatgcgc
    11101 gaagccagtt gttcctgcac acgctgaatt tctccgacgc cagttaaaaa taacagtaat
    11161 gatccacttt cctgacgcag catttcggcg gtggcaaccg cgacggcttc gtcaaaacgc
    11221 tgatgcgcgg gcagcggtaa ataacggcgt tcaaccggaa acgagcgccc ttctgagatg
    11281 acgacgggcg cttctggcag catttgctgc aagcggtcgt tgtccagcgt agccgacata
    11341 atcagcagtt taaggtcatc acgcagacct tgttgcacat cgagtaacag cgccaacgcc
    11401 aaatccgcct gcaagctgcg ctcatgaaat tcatcaagga tcaccagtcc aacaccgctc
    11461 agttccgggt cacgctggat catccgcgtc agcacgcctt cggtaactac ttccaggcgg
    11521 gtattcggcc cgacgcagtt ttgcgcacgc atccggtagc caacggtatc gcctggcttt
    11581 tcgttaagca gctccgccag ccgttgcgcg acgttacgcg ccgccagacg acgcggctcc
    11641 agcaggataa tttttccgtt aatgccggga tgcgccagca attgcagcgg cagccaggtt
    11701 gatttcccgg ccccggtcgg cgcacttaat aatacctgcg gcgcacaatc gagagcggta
    11761 agtaattcag gtaagacggc agcaacgggc aacgacgaca caaaacgctc cagagggtta
    11821 acattcttcg cgccacattg tagcatcgcg gtaattcata accgagtgcc tcacatgtct
    11881 gaaccgcaac gtctgttctt tgctatcgac ttacctgcag aaatccgcga acagattatc
    11941 cactggcgcg ccacacactt cccacctgag gcgggacgtc cggtcgccgc cgataatttg
    12001 catctgactc tggcattttt aggcgacgtg agcgccgaga aagagaaggc gctttctctt
    12061 ttagccggac ggattcgtca atctggtttc acactcacgc ttgatgacgc cggacaatgg
    12121 ctgcgttcgc gtgtggtgtg gttagggatg cgtcagcctc cacgcggctt aatccagctg
    12181 gcgaatatgc tccgttcaca ggctgcccgc agcggttgtt ttcaaagcaa tcgtccgttt
    12241 catccacata ttaccttatt gcgcgacgcc agcgaggcgg tgacaatccc gccgccaggt
    12301 tttaactggt catatgcggt gacggagttc accctttacg cctcctcgtt tgcccgtgga
    12361 cgcacacgct acacgccgct aaaacgctgg gcgctaacgc aataacaagg attgtcgcaa
    12421 tggaattttc tccccctcta cagcgcgcga cgctaattca gcgttacaaa cgttttttag
    12481 ccgatgtgat cacacccgat ggtcgcgaat taacgctaca ctgcccgaat acgggtgcga
    12541 tgaccggttg tgcaacgcct ggcgataccg tctggtattc gacttcagac aacaccaaac
    12601 ggaaataccc acacacctgg gaattaactc aaagccagag cggcgcattt atttgcgtca
    12661 acacgctttg ggctaacagg ttgacgaaag aggctatcct taatgaatca atttcagaac
    12721 tgtcaggcta tagctcgctg aaaagcgaag taaaatacgg cgcagaacgc agccgtattg
    12781 actttatgtt gcaggcggat tcgcgtccag actgctatat tgaagtgaaa tcggttacgt
    12841 tagcggagaa cgaacaggga tattttcccg atgcggtcac tgaacgaggt cagaaacacc
    12901 ttcgggagtt gatgagcgta gcggctgaag gccagcgtgc ggttatcttt ttcgccgtgc
    12961 tgcattcagc cattacacgg ttttcacccg cgcgccacat cgatgagaaa tacgcgcaac
    13021 tattgtcaga agctcaacag aggggggtag aaattctggc ttacaaagcg gaaatttctg
    13081 ctgaaggcat ggctcttaaa aaatcactgc cggttacatt gtagtaaagt aagtaactgg
    13141 ttaatttaca ttctggtcgc gtgcgcaaat acgcttttcc tcacacagtt gtcaagtgtt
    13201 acgtttagat aattgctatc cggaaaagca tctgctattt atagcggcct catttttccc
    13261 ccgaacatgg ggatcgatag tgcgtgttaa ggagaagcaa catgcaagaa gggcaaaacc
    13321 gtaaaacatc gtccctgagt attctcgcca tcgctggggt ggaaccatat caggagaagc
    13381 cgggcgaaga gtatatgaat gaagcccagc tggcgcactt ccgtcgtatt ctggaagcat
    13441 ggcgtaatca actcagggat gaagtcgatc gcaccgttac acatatgcag gatgaagcag
    13501 ccaacttccc ggacccggta gaccgtgcag cccaggaaga agagttcagc ctcgaactgc
    13561 gtaaccgcga tcgcgagcgt aagctgatca aaaagatcga gaagacgctg aaaaaagtgg
    13621 aagacgaaga tttcggctac tgcgaatcct gcggtgttga aattggtatt cgccgtctgg
    13681 aagcgcgccc gacagccgat ctgtgcatcg actgcaaaac gctggctgaa attcgcgaaa
    13741 aacagatggc tggctaatta cagccgttcc atcacgttta ccacaggcgg gaaatgctcc
    13801 cgccttattt tttgttcaaa gagatgacag acacacacta tattggccgc ttcgccccct
    13861 ctccttccgg cgagcttcat tttggttctc tgattgctgc gcttggcagc tatttgcagg
    13921 ctcgcgcccg gcaaggtcgc tggctggtac gtatagaaga tatcgacccg cctcgtgaag
    13981 ttcccggtgc cgcagaaact atcctgcgcc agctggaaca ttacggtctg cactgggacg
    14041 gcgatgttct ctggcaatcg caacgtcacg acgcctatcg tgaagcactc gcctggttac
    14101 atgagcaagg gctaagttat tactgcacct gcacgcgtgc gcgtattcaa agcattggcg
    14161 gtatttacga cggtcattgc cgggtgttgc atcatggacc agacaacgcc gcagtgcgta
    14221 tccgccagca gcatccggtc acgcaattta ctgaccagct gcgcggcatt attcacgccg
    14281 acgaaaaact ggcacgggaa gattttatta ttcatcgccg tgatggtttg tttgcctata
    14341 acctggcggt ggtggtggat gatcacttcc agggcgtgag cgaaattgtc cgcggggcag
    14401 atttgatcga accgacagtg cggcaaatct cgctgtacca gctttttggc tggaaagtgc
    14461 cagattacat tcatctgccg ctggcgctta atccacaagg cgctaaactt tccaagcaga
    14521 atcatgcgcc tgcgttgccg aaaagcgatc cacgcccggt actaatcgcg gcacttcaat
    14581 ttctgggtca gcaggcagaa gcacactggc aggatttcag cgtcgagcaa atcctccagt
    14641 cagccgtcaa aaactggcgg cttaccgccg tgcctgagtc agcaattgta aattcaacat
    14701 tctcaaatgc gtcatgctga gctatgatta gccgctattt ttttgtcctg aatgatgttt
    14761 gacactaccg aggtgtacta tttttacccg agtcgctaat ttttgccgca aggtgctaag
    14821 ccgcgaggaa agcgaggctg aacaggcagt cgcccgtcca caggtgacgg tgatcccgcg
    14881 tgagcagcat gctatttccc gcaaagatat cagtgaaaat gccctgaagg taatgtacag
    14941 gctcaataaa gcgggatacg aagcctggct ggttggcggc ggcgtgcgcg acctgttact
    15001 tggcaaaaag ccgaaagact ttgacgtaac cactaacgcc acgcctgagc aggtgcgcaa
    15061 actgttccgt aactgccgcc ttgtgggtcg ccgtttccgt ctggctcatg tgatgtttgg
    15121 cccggagatt atcgaagttg cgaccttccg tggacaccac gaaggtaaca tcagcgaccg
    15181 cacgacctcc caacgcgggc aaaacggcat gttgctgcgc gacaacattt tcggctccat
    15241 cgaagaagac gcccagcgcc gcgatttcac tatcaacagc ctgtattaca gcgtagcgga
    15301 ttttaccgtc cgtgattacg ttggcggcat gaaggatctg aaggacggcg ttatccgtct
    15361 gattggtaac ccggaaacgc gctaccgtga agatccggta cgtatgctgc gcgcggtacg
    15421 ttttgccgcc aaattgggta tgcgcatcag cccggaaacc gcagaaccga tccctcgcct
    15481 cgctaccctg ctgaacgata tcccaccggc acgcctgttt gaagaatcgc ttaaactgct
    15541 acaagcgggc tacggttacg aaacctataa gctgttgtgt gaatatcatc tgttccagcc
    15601 gctgttcccg accattaccc gctacttcac ggaaaatggc gacagcccga tggagcggat
    15661 cattgaacag gtgctgaaga ataccgatac gcgtatccat aacgatatgc gcgtgaaccc
    15721 ggcgttcctg tttgccgcca tgttctggta cccactgctg gagacggcac agaagatcgc
    15781 ccaggaaagc ggcctgacct atcacgacgc tttcgcgctg gcgatgaacg acgtgctgga
    15841 cgaagcctgc cgttcactgg caatcccgaa acgtctgacg acgttaaccc gcgatatctg
    15901 gcagttgcag ttgcgtatgt cccgtcgtca gggtaaacgc gcatggaaac tgctggagca
    15961 tcctaagttc cgtgcggctt atgacctgtt ggccttgcga gctgaagttg aacgtaacgc
    16021 tgaactgcag cgtctggtga aatggtgggg tgagttccag gtttccgcgc caccagacca
    16081 aaaagggatg ctcaacgagc tggatgaaga accgtcaccg cgtcgtcgta ctcgtcgtcc
    16141 acgcaaacgc gcaccacgtc gtgagggtac cgcatgacag tggcgtatat tgccataggc
    16201 agcaatctgg cctctccgct ggagcaggtc aatgctgccc tgaaagcatt aggcgatatc
    16261 cctgaaagcc acattcttac cgtttcttcg ttttaccgca ccccaccgct ggggccgcaa
    16321 gatcaacccg attacttaaa cgcagccgtg gcgctggaaa cctctcttgc acctgaagag
    16381 ctactcaatc acacacagcg tattgaattg cagcaaggtc gcgtccgcaa agctgaacgc
    16441 tggggaccac gcacgctgga tctcgacatc atgctgtttg gtaatgaagt gataaatact
    16501 gaacgcctga ccgttccgca ctacgatatg aagaatcgtg gatttatgct gtggccgctg
    16561 tttgagatcg cgccggagtt ggtgtttcct gatggggaga tgttgcaaaa tagacttact
    16621 atcctgaata cttcacaacc tgtaatctgg taatttcaat tatttatatt tgcaagtcag
    16681 gtcaaaaggt tattgatgat agaaggtata ccgccctctt taaaataaaa ttatttctta
    16741 taataatcat aattttgaat ttcctcactt taaatattct aaaatcattt tttaaattat
    16801 ctaacttgac acccataatc ataaataaaa caaataaatt cactgatatt cactaaaaaa
    16861 tagactatac acaaaaaaaa tccataaacc aaataaatat attcagccaa gtgatattta
    16921 tcaaatactt acataataac cacatattca gtaatatacg ccagctatat taactaataa
    16981 cttttttgta aggacacatg taatgaaaaa aacattactc gccgccgcac tggtgatggt
    17041 ttctggttcc gctttggctg tagatggtgg tcatattgat tttaacggtc tggttcagtc
    17101 tggtacttgt aaagtcggcg tggtagacgc gggtatgcac ggcattacta ccgatggcgt
    17161 agtaacactg gataccgcaa atgttactga taactttgcg gaagttaacg cgactgcagt
    17221 tggtttgttg ccaaaagaat ttatgatttc tgtaaattgt gaacctggta agacagaagc
    17281 ctctttaact atgggttccg caagctacgc aaacactagt ggtactttga ataacaatat
    17341 gaacatcact gtaaatggca ttcagccagc agagaatgtg aatattgcag ttcataacat
    17401 gaccaacaaa tctggcgcag ctgagattaa acaggtacac atgaacaact ctgcagaaaa
    17461 acatctgctg actttggatg ctaatggcga cggtcaatat gtgtttaatg cctcctatgt
    17521 aaaagcaccg aacagccagg atgtgacttc tggacacgtt accactaacg cactgtacac
    17581 cattacttat aactaatggt gcaataagca taaggtttgc ctgcgggcaa accttatttt
    17641 tccgttttca cataattaca caatagcatg gagtgcttat gtttcccatt ataaaaaaaa
    17701 ccgtcagcgc gttatttatt agtacgttgt taaccagtgc acctgcattc gccgatatca
    17761 taatttccgg cactcgtatc atatataatg cagataaaaa agatgttaac gtccgtctgg
    17821 aaaacaaagg caatcggccc ttgctaattc aaaactggtt ggatacaggt gatgacaatg
    17881 cggatcccag ccagatcaaa gttccgttta cttctacgcc gcctgtatct cgcattgaat
    17941 ctaaacgtgg gcaaacggta aaggtgatgt ttactggaac aacacaactt cctgcggacc
    18001 gggaaagtgt tttctggttt aacgtgctgg aagtcccgcc caagccgaaa gatgccgaat
    18061 cagacaaaaa cttattacaa ctggctttcc gtacccgtat caaacttttt tatcgcccaa
    18121 gtggattaaa aggtgaaccc gctgaagcac cagccaaact gacgtggaag ttaaataatg
    18181 cgcagcttca ggtaaacaac ccaacccctt actacgtttc attcaatgac gtcgcaattg
    18241 aaagcggcgg taagagctac aaagtcgact ccagcatggt tgcgcctttt ggtcaggtta
    18301 gttttgccgt gacagatata gcgagcagtg tgtcttcagg aaaagttatt tacaaagcca
    18361 ttaatgatta tggtggcaat attgatggct ccacatcgct ttaatcacta aacggattaa
    18421 ctttaaatcg ccaggaacgc aaccttgtat acgttaactc atcagaaaag ccgtctacca
    18481 aaaacaacac ttctggctgc atgttgtgct tttttttata gcagtaacgg cgcggcaaca
    18541 gaaagcgtgg aatatgacag ttcctttttg atgggtactg gggcatcgag catcgatgtc
    18601 aaacgttatt cacagggtaa cccaacgcct cctggcgttt ataacgtccg tgtgtttatc
    18661 aaccaacaat ccgttgccag tttagaactt ccttttgttg atatagggga aaacagcgcc
    18721 gcggcctgta taacacttaa gaatctggca caactgcata ttaagaagcc agaataccca
    18781 attacactgc ttgccagaga aggcgaagaa ggcgattgcc tggacatcaa aaaatcgatc
    18841 gagcaagcgg aagtcagcta tgacggcagt gagcagcatt tggaaatctc ggttccgcag
    18901 gcttacgtct ataaaacata tggcggttat gttgacccct cactttggga gtcgggtatt
    18961 aatgccgcca tgctttctta ctcattaaac gcctaccact ccgactctaa aaatggcaac
    19021 cgggacagta tatacggagc cttcaatacc ggtctgaatt taggagcctg gcacctgcgt
    19081 gcgcgcggca actataactg gtcacaagat aacggtaaca gttttgactt tcaggatcgt
    19141 tacttacagc gtgacatccc ggcaatacgc tcacaagtga tcgtgggtga tgcctatacc
    19201 accggtgaaa ccttcgactc agtcaatgta cgtggtatgc gtctttatag tgatagccgt
    19261 atgctaccat ccgcactggc cagctatgcg ccaatcattc gtggcgtagc aaattctaac
    19321 gccaaagtga cagtgaccca gaacggttac aaaatctatg agtccaccgt tccaccgggt
    19381 gaattcgtga ttgacgatct cagtccttca gggtttggta gcgaattggt cattactgtt
    19441 gaagaagcag atggttctaa acgctcattt acccaacctt tctcttctgt cgtgcagttg
    19501 cagcgccctg gcgttggccg ttgggatatt agtgccggac aaattatcga tgacagcttg
    19561 cgtcatgaac ccaatatggc acaggcctct ttttactacg gtttcaataa tttattcacc
    19621 ggttataccg gagtccaaat tacagataac gactatatgt ctggcctttt ggggctaggg
    19681 atcaatacca gtattggtgc ctttgcggta gatattacac atgcccgtac cgaaattcct
    19741 gacgataaaa catatcaggg gcaaagttat cgtataacct ggaacaaatt aatcgaagct
    19801 acaagcacct catttaacct tgctgcctat cgttattcc
//
LOCUS       DACAPB010000074        19599 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.074, whole genome shotgun sequence.
ACCESSION   DACAPB010000074 DACAPB010000000
VERSION     DACAPB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.074"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..196)
                     /gene="cvaC"
                     /locus_tag="IT904_002386"
     CDS             complement(<1..196)
                     /gene="cvaC"
                     /locus_tag="IT904_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
                     /protein_id="HAP3192157.1"
                     /translation="MRTLTLNELDSVSGGASGRDIAMAIGTLSGQFVAGGIGAAAGGV
                     AGGAIYDYASTHKPNPAMSPS"
     gene            complement(174..410)
                     /gene="cvi"
                     /locus_tag="IT904_002387"
     CDS             complement(174..410)
                     /gene="cvi"
                     /locus_tag="IT904_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HAP3192158.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTFC
                     FSILMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            complement(874..1155)
                     /locus_tag="IT904_002388"
     CDS             complement(874..1155)
                     /locus_tag="IT904_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192159.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            1328..1495
                     /locus_tag="IT904_002389"
                     /pseudo
     CDS             1328..1495
                     /locus_tag="IT904_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-B"
     gene            complement(1513..2040)
                     /gene="cbi"
                     /locus_tag="IT904_002390"
     CDS             complement(1513..2040)
                     /gene="cbi"
                     /locus_tag="IT904_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HAP3192160.1"
                     /translation="MTSNKDKNKKANEILYAFSIIGIIPLMAILILRINNPYSQVLYY
                     LYNKVAFLPSITSLHDPVMTALMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQ
                     SCFWGAVFYAILIYITLFYNLELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAI
                     LLMPLLVIKYFKGRQ"
     gene            2284..3099
                     /gene="cma"
                     /locus_tag="IT904_002391"
     CDS             2284..3099
                     /gene="cma"
                     /locus_tag="IT904_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HAP3192161.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFSKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGVIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(3149..3502)
                     /gene="cmi"
                     /locus_tag="IT904_002392"
     CDS             complement(3149..3502)
                     /gene="cmi"
                     /locus_tag="IT904_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HAP3192162.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
     gene            complement(3680..4471)
                     /locus_tag="IT904_002393"
     CDS             complement(3680..4471)
                     /locus_tag="IT904_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP3192163.1"
                     /translation="MSGPQLLSLPGSFLTPPATLPVAIDYPAALALRQMALVQDELPK
                     YLLAPEISALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATL
                     KQRTEKATRTAGRVPAGSQVHRLVPLSDTQYVSQLQMMVATLKIPLERRNKRTGRMEK
                     ARIWGITDRTVRTWLNEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQS
                     LMGHKSISSTEVYTKVFALDVAARHRVQFSIPESDAVTMLKNRHA"
     gene            complement(4468..5157)
                     /locus_tag="IT904_002394"
     CDS             complement(4468..5157)
                     /locus_tag="IT904_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192164.1"
                     /translation="MADWFIATEGVKVVKDSASLGPQIITAVSTIASAVGAVFLTHHF
                     TLRREERTAAAKLASERLYISTELVFLLERFAQRCAPAATATVEHDQDGRGEAEFLLP
                     EIDYSPVSGNWRSLPSGLMYRLSELSVLSYEAVVCVDNVFVEDTPYGGAGEYSLHKNA
                     AMLGVKALRLSRELRMLCGLPLHGLSDTLSPTRLVLLKARGKTLQKEYEMVKKSKKTE
                     QEIEDFIKGTS"
     gene            complement(5201..5551)
                     /locus_tag="IT904_002395"
     CDS             complement(5201..5551)
                     /locus_tag="IT904_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192165.1"
                     /translation="MFTAFNERNDFSYAFEKIRNAISAPGENNVYAATELGLGILLRK
                     YEQFRRELDAAGELGNWEYDLDTYNHCIAVLQRYFTGNPSGLTERDARIYSHYLQTEH
                     KGFVKLAEELAADR"
     gene            6096..7184
                     /gene="pifC"
                     /locus_tag="IT904_002396"
     CDS             6096..7184
                     /gene="pifC"
                     /locus_tag="IT904_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor PifC"
                     /protein_id="HAP3192166.1"
                     /translation="MLSQLNLRFHKKLIEALKTRAGRENTSVNALAERFLDDGLKTVA
                     PGDGYFQLIADPEATVRQLYRHIILGQTFGTSALSRDELRFVLVHVREAFLRGHNRLA
                     TLPALDTLLDITGNLLAWQVEHDRPVDGHYLKGIFRLAGKNWTEEFEAFRAALRPVVD
                     QMYAEHLLRPLESDCFGLAEVPDAVLAEIFTLPRLKAVFPLMLRGLDWNTEQARTLAQ
                     ELRPVISAVTETIEAGTLRLEIRVDGQHPGERPGAWYTTPRLHLLITGQDFVVPYGWE
                     ALSELLGLFTLYARHPEALTHGHQGERVMFSPPGNVTPEGFFGIDGLRIFMPAEAFET
                     LVRELATRCQEGPLAEALTGLRCLYGDL"
     gene            7186..9411
                     /locus_tag="IT904_002397"
     CDS             7186..9411
                     /locus_tag="IT904_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage T7 exclusion protein"
                     /protein_id="HAP3192167.1"
                     /translation="MKILRQLWNQKGLDAAVEDVPEDRYGFGNIAENISRSILTLPLE
                     ASNVVGIEGAWGSGKTSLLNLILRNLALKKDAHTHVLHISPWLSGGSPVEALFLPVAT
                     VIQQEMEIRYPPKGFKKLWRKYLLSPEAQKVIEYAQDTSSRVLPLVQYIGQFSSIINW
                     IAGGIKVFSDSRLAVDQKTTTKLRAEIAGQLVSLDLKFIVVMDDLDRLEPSQVAEVFR
                     LVRAVADLPRFTHILCYDRQIITHAVEHALNIEDGSRYLQKIIQLSFKLPRPEAFDLR
                     NEFRQRAEALYQQINNQPPDSGMVRDLIAVTDTYGAALSTPREIHQAINSLIFLYPGM
                     RDFVYFPDLCLLQLIRVTNPALYDWTEHYLTERSVIETGQGMLSDGEKADFREGLIRC
                     MKTFRASNADSFLTLADWIPGISGHNDEYLNLFEPVSEDFRHIQTTGKRLSSLTHWRY
                     YFAFSSPQNVLPPEFFRQLFEQAGVSEKQQQLSELLLSKINSVGSLSGTWFEHILSRL
                     TPGLIRERNFEECAGLVHFFFDHTDEVSTRFSIRNPWFSLREMAINEVVRHLLKHMQD
                     IDETRTITLMEKLIVTGASPFWIADFMRDLIWEHGLAQNAVPSPSDALFSRDITERLR
                     DRFAERMNQPELQQQLLLRKSLLGYLYAWRDMSSGETVKQWVREVTTTDEGLVNLLIR
                     LQTSVFSSHRGAYRRIARDQVSPFFDDWPAVEEKLKVMLSGNELTPEQEALKTALEND
                     D"
     gene            complement(9461..10360)
                     /locus_tag="IT904_002398"
     CDS             complement(9461..10360)
                     /locus_tag="IT904_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HAP3192168.1"
                     /translation="MAIDIFQSLKNCVFDLQRADVQNYQQPLKQLARLLNSENLQSVN
                     AHLTRNVELDTFLARSEDTESSMAGSAVLQWPDEPADILGLKLLLIEKMADDNNFSFN
                     FCHTFFYDRNIIESIRKFTSSLVAPFVRDYQLYVENQHDPEPAVFRPVSRKIFIVHGH
                     DNDALQSVARFISRIGLEEIILSERPDGSRTIIEKFEAESGDVSFAIVLMTPDDSGSA
                     LASESTRLRARQNVLYELGYFAGKLGRGKVLVLRKGDIEIPSDLAGVLYTELDEHGGW
                     KRKLLSELAYAGVPFDKDKALSA"
     gene            complement(10350..10640)
                     /locus_tag="IT904_002399"
     CDS             complement(10350..10640)
                     /locus_tag="IT904_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192169.1"
                     /translation="MSVKITGLDKMQKQLKEVERATEALNGSYDVHFDANDPVSIENA
                     IQEAYSMVYERASGYATNPMVSPLIEHMKENLRQQILDRAEQQRQESGQDGN"
     gene            10936..11166
                     /locus_tag="IT904_002400"
     CDS             10936..11166
                     /locus_tag="IT904_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAP3192170.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            11163..11579
                     /locus_tag="IT904_002401"
     CDS             11163..11579
                     /locus_tag="IT904_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HAP3192171.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(11741..13879)
                     /locus_tag="IT904_002402"
     CDS             complement(11741..13879)
                     /locus_tag="IT904_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP3192172.1"
                     /translation="MDIEIRHCNNIVRAHITLTADKLNIKFAPNGTGKSTLSRAISCA
                     ARDDIQGLQALMPFRLRGENPDSTGPIVIGADGIGDVMCFNEEYVSQFTFQPDELISD
                     SFNILIRNQAHAEREREIEEMTQKIRAVFTDHTELNSLIDHLQELSNAFRSTSSGISR
                     SSTGMRGLSGGNKIHHIPAGLENYQPYIRSERRVEWIDWQTKGLEFSPLSDGCCPFCT
                     GDITGKEAQIRQVREEYDKSTIKNLTAIIRLVENLGNYLTESARERLLAITMLQNGPE
                     AEHIEYLVALKRQTDTLTEKLTALRGLNVFSLQEQQNVREVLTARLIDLQFFPDLQSE
                     LMQGITDRLNAALMDLINLAGPLQGKINRHRDSMIRLIAQHKTNINNFLTYAGYKYRV
                     DIAGEGEQRKLRLRHIDFDGYVSGGSQHLSYGERNAFAIVLFMYECLSKNPGLIILDD
                     PISSFDKNKKFAILEMLFRRASGECLKNRTVLMLTHDVEPVIDTLKSVRRLFSNQVTA
                     SCLRLSAGVIEELPVNDGDIMTFMQICKSITASADCEEIIKLIYLRRYFEIVDERGDA
                     YQLLSNLFHRRVVPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALV
                     SSPDEIKNLYRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRF
                     DLIPEYVIMECDKLIALPPAANQSSVARIA"
     gene            14233..14490
                     /locus_tag="IT904_002403"
     CDS             14233..14490
                     /locus_tag="IT904_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192173.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            14490..15080
                     /locus_tag="IT904_002404"
     CDS             14490..15080
                     /locus_tag="IT904_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192174.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            15343..16899
                     /locus_tag="IT904_002405"
     CDS             15343..16899
                     /locus_tag="IT904_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP3192175.1"
                     /translation="MAEKNKKTITGQVLNSIKINKLKCINGLNEIIFKPHALTAILGP
                     NGSGKSTILHAIASIYMPEEGFPGEDHRLMHFFPRSPHAEWNGSDFIVNLTYRKDGVM
                     IENELKNYGKADIRGSRWIQIYARRPLREVYYLGIDKCVPIIESEKKNNIQYETSSVS
                     NDLITNILHYASYILNKPYTSFNQHQQPNGKILIGVESGGLAYSSLSMSAGEQKIFLI
                     LETILKADKNALILIDELDLLLHDEALKKLIEVISSHAKDKNKQIIFTTHREMITTLS
                     DKINIRHVVNIQGRSYSFEETKPDAINRLTGESTTPIEIYVEDDLAVAIINKICSSLK
                     ASKYVKIFKFGAASNAFTLLASTLIRGDNLSGKLYILDGDKYSTENEKKTALDKVFTG
                     TESRTYELKAAAEGKVKQFNLPNGVKPEQYIHYLITNVPLDGLGGEYLEIIEAARDIR
                     VELDAHNYISNILTKLGIDRPSGLTRVMDLASRHPEWHQYVSEVTDWLQPVVSDLMER
                     LPENDTVDIT"
     gene            17091..17708
                     /locus_tag="IT904_002406"
     CDS             17091..17708
                     /locus_tag="IT904_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HAP3192176.1"
                     /translation="MGKYQEEKKTPIIMVKKRRTLLPPSLSEKTGVIARSETEKMEES
                     VSEGISSPAVDTHTPDAPACKKKKKRRRFPRQPHWTHEFTHDCVEKVKSLFPHLRAEG
                     GGFLPLKIGITNDFAAFLTEHPETELTLDEWSCAISCITTRQVYLQRTAVAGIPRYGL
                     DGLPAGQVSECDALNARRWLAVREQQKLKMKTMQEQSASTEKTER"
     gene            complement(18001..19038)
                     /locus_tag="IT904_002407"
     CDS             complement(18001..19038)
                     /locus_tag="IT904_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP3192177.1"
                     /translation="MNSWIPMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLASLLGAVTPFCSCSTVPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLIWVTFGWKVTLLYAIIAAGVSVLASIVLDLLGFERHIVDHKNSASSGCTTKC
                     DDSEASVKRTVKNEQNVSACCSSISSEQPAVSCCSSENLGNVNLTTNTTSGPIKLAMK
                     DALQQFKDVLPYLLLSVLIGSFIYGFIPSEWIAAHAGADNPLAIPLSAVVGIPLYIRA
                     EAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIIAFLTVILGMA
                     ILMGYLTQFLF"
     gene            19143..19466
                     /locus_tag="IT904_002408"
     CDS             19143..19466
                     /locus_tag="IT904_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3192178.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PSSTLSHHISALVSVGLVTQNRESRTLMCVSQYEILEAIIEFLREECCVNSKTDVAEP
                     AGKNG"
BASE COUNT         5409 a         4552 c         4462 g         5176 t
ORIGIN      
        1 cggatggaga cattgcagga ttaggtttgt gagtggatgc atagtcatat attgcacctc
       61 cagccacacc cccagcagct gctccaattc ctcctgcaac aaattgtccg gatagtgttc
      121 ctatagccat cgcaatatca cgccctgaag caccaccaga aacagaatct aattcattta
      181 gagtcagagt tctcatatga tctccttttt atcttatcgg atattgaata ataattatca
      241 ccaacaaagt aacatattgc agacattaat atagagaagc aaaatgtatg catggataaa
      301 aagtcctttc ctctaaaaac acaatcatat atagctaatg caatatatat tgcggtggca
      361 tttattataa atgcaaataa caactctaat tttgttcttt ttctatccat tactttttat
      421 cccattactt tctatcccat taccatacaa acactaacga taatgattat cgttcacata
      481 gtcgagagtg aaggggaggg ggccctcacc ccctataagg ggtccgcttg gaaaacggat
      541 ttccccacgt caagagaatt gagttgaacg agtggttaca tccaagccag ctaactcact
      601 gaacgtaaag gatgtacgaa atgctcctac cgtccgataa ccatttggct tacttccgta
      661 cattcccttg cgtagatgtt cagaattgta cgatttcgga caccaaaacc gctgttgtcc
      721 cgccattctg gcgattcctg aaagtcattt gactaaaaaa tagtcttaac gtggtttatt
      781 ttccgaattc caagcggccc gccctacatt tatacaatgt ccggcagggc ataaaagggg
      841 cgaaagaaaa tacctgggcg aagtcgctga acatcattct ggcgatttca ataaaggtac
      901 ttgctcacgc tctgtcattt tctgaaactc ttcatgctgc atttcgattt tccttctaag
      961 atcataactc gcatacctgt tctttttatg tatatctata tctatttttg tgacgagttc
     1021 aaaccacaat gttcgcactc cccatgcagc gttatgaaac atttccgcaa cggctataga
     1081 gtctgtatta actgcttcag tcatgtagtc gctaatacgt tcagcggtaa ttttcgttat
     1141 ttcatcgtaa ttcattttat ctcctacaag attatttaat ggaatgcaaa atatctggcc
     1201 tgataataat gttccagaat taatatctgc aggctatgtt aagttgtccc cacacacaat
     1261 ctacctgttt tctcgtgtgt gccacaggag caatacataa atagtctggc cattaagttc
     1321 gtaaccagct gctgtagctc ttagtttgtt ttctttgaca ttaggctcgg cccttatagc
     1381 ctttggtctt ccggccacag ttgttggttt tgttggcgta gttattgcag gtgctattgg
     1441 tgcatttatc gatgataaat ttgttgatga gttgaatcac aagatcataa aataattaaa
     1501 aggaaggcag tgttactgcc tccctttaaa atatttaatg acaagcaatg gcatcagtaa
     1561 aatcgcatat gtcatggtta aaacagtaaa gtaaatggag cagtataaaa taaaaagagt
     1621 gatgacatta tgagataata atttaaaaaa accacctgct gttgttagtt ccagattata
     1681 gaacagtgtg atataaatca gaatggcata aaaaacggcc ccccagaaac aggattgcac
     1741 aacaagtttt cttgttactg gctttatgat ttctcttgtc ttatatgtgc aaagaaaaac
     1801 gagaataccc ataactggtg ctgtcttgtt gtagtttgac ataagtgctg tcatgacggg
     1861 atcatgcaat gatgtaatag aagggagaaa tgcaacctta ttatataagt agtacagcac
     1921 ttgagaatat ggattattta tccgaagtat taatatagcc attaatggaa taataccgat
     1981 tatggaaaat gcatataata tttcgtttgc tttcttgttc ttatctttat tgctggtcat
     2041 atatatacct ttttgtattt ttatatatag attatatatc agagttctca atatatttcc
     2101 agtatttaat ggagtgtaca gatatatatc ttatatatgg tagttcaggt gcttccctac
     2161 catttttaac ggcatatccg gcaaacaatg ttatggggag cctctctcat ggggttcgtt
     2221 cgccggtgta agcgttagcc agccgcgaac tctattatct tattaactta taaggagtta
     2281 tgtatggaaa ccttaactgt tcatgcacca tcaccatcaa ctaacttacc aagttatggc
     2341 aatggtgcat tttctctttc agcaccgcat gttcctggtg ctggacctct tttagtccag
     2401 gttgtttata gttttttcca gagtccaaac atgtgtcttc aggctttaac tcaacttgag
     2461 gattacatca aaaaacatgg ggctagcaac cctctcacat tgcagatcat atcgacaaat
     2521 attggttact tctgtaacgc cgaccgaaat ctggttcttc accctggaat aagcgtttat
     2581 gacgcttacc acttctcaaa accagcgcca agtcaatatg actatcgctc aatgaatatg
     2641 aaacaaatga gcggtaatgt cactacacca attgtggcgc ttgctcacta tttatggggt
     2701 aatggcgctg aaaggagcgt taatatcgcc aacattggtc ttaaaatttc ccctatgaaa
     2761 attaatcaga taaaagacat tataaaatct ggtgtagtag gtacattccc tgtttctaca
     2821 aagttcacac atgccactgg tgattataat gttattaccg gtgcatatct tggtaatatc
     2881 acactgaaaa cagaaggtac tttaactatc tctgccaatg gctcctggac ttacaatggc
     2941 gttgttcgtt catatgatga taaatacgat tttaacgcca gcactcaccg tggcgtcatc
     3001 ggagagtcgc tcacaaggct cggggcgatg ttttctggta aagagtacca gatactgctt
     3061 cctggtgaaa ttcacattaa agaaagtggt aagcgataat ataagggccg ggattccggc
     3121 ctttttatgc agcctttata acagtgtatt agtcattcgc atattcaaca tatccctctt
     3181 cacaatggta catgccataa tacattctct taccctccgg accttcagct ttaaatggga
     3241 caagaagtgt tttttcatct tcaacatctg ttaaacttct ctcaatcttt tcccatgtaa
     3301 ttttcgggat ttctgtacca agaagttgta catcgttgtc ccagcgtttc attctttgca
     3361 ggtaatcatt ttttacaaac gttctggcct gttcatcgct gacctgatag catgccggac
     3421 ctttatcttc agaccagaaa aaaacagcag caagagcaat aatcgttaaa ataaaaataa
     3481 atttcatgct aattactttc ataaaatctc cacaacttct gtcgttcatt cgatataaaa
     3541 aaacattacg aatatatccg tacaatgtga gcatactgtg aagtggcttt aacaattatg
     3601 aaataataca agagatcaca tattgcagtg gtctcttctt ttatagaata aatcacaatt
     3661 cataattatg atttgtgact tatgcgtgtc tgtttttcag cattgtgaca gcatcggact
     3721 caggaatcga aaactgcacc cggtgccgtg cagccacatc cagcgcaaac accttcgtgt
     3781 acacctctgt cgaactaatc gacttgtgcc ccatcaggct ctgcagcacc ttcagcggaa
     3841 taccagcata cagcatatgc atcgcgtagg aatggcggaa agtatgtggg gtaaccggaa
     3901 cagagaacgt cacgccgtcg gccgcagcgg cctctacagc ttcattcagc caggtgcgaa
     3961 ctgtgcggtc ggtgattccc cagatgcgcg ccttctccat tcttccggtt cgtttgtttc
     4021 tgcgctccag tgggattttc agcgtggcca ccatcatctg cagttggctg acgtattgtg
     4081 tatcggacag gggaaccagg cgatgcacct gactgccagc agggacacgg cctgccgttc
     4141 tggtcgcttt ttcagtccgt tgtttgagcg tggccagctg tacgaacgga tatggcggcg
     4201 ccagagaaaa atctccccgc gtcagcgcca gcgcttcgtt tatacgtgcg cccgtgttcc
     4261 acagggtggc cagcaacatt ttgcggtgca gatccggtac atagtggagc agggcgctga
     4321 tctccggcgc caacagatat tttggtaatt cgtcctgcac aagtgccatc tgacgtagtg
     4381 ccagcgcagc cgggtaatca atggcaacag gtaatgttgc cggaggcgtt aaaaaagaac
     4441 caggcaggga aagcaattgt gggccactca tgatgtcccc tttatgaaat cctcaatctc
     4501 ttgctcggtc tttttagact tctttaccat ttcgtactcc ttttggagtg ttttacctct
     4561 ggctttcaac aataccaacc tcgttggcga tagtgtatcg gaaaggccgt gcagagggag
     4621 gccacataac attctcagtt ctcgtgataa ccgcagtgct tttactccca acatagctgc
     4681 gtttttatgc aaactatatt cacccgctcc accataagga gtatcttcaa caaacacatt
     4741 atctacacac acgacagctt cgtagctgag aacagagagt tcgctgagcc tatacatcag
     4801 gccagaagga agggagcgcc agttaccaga taccggggaa taatcgattt ccggtaataa
     4861 gaactcagct tctcctctgc catcctgatc gtgctccaca gttgctgttg ctgctggagc
     4921 gcaacgctgc gcaaatcgct caagtaaaaa aaccagctct gtactaatgt aaagacgctc
     4981 gctggccagc ttcgctgctg cagttctctc ctcacgtctg agtgtgaagt ggtgagtaag
     5041 gaacaccgct ccgacagcag atgcgatggt cgatacagct gtaattatct gcggccccag
     5101 actggcgctg tccttcacca ccttcacgcc ttcggtcgca ataaaccagt cagccatgtt
     5161 tattccttta ttgttgagaa atcccgcctt ctgtatgtat tcagcggtcg gcagccagtt
     5221 cttcagccag cttcacaaag cctttgtgct cagtctgcag gtaatggctg taaatccggg
     5281 catcacgttc tgtgagcccg gatggattgc cggtgaagta acgttgcagc accgcaatac
     5341 agtgattgta ggtatcgaga tcatattccc agtttcccag ctcaccggcc gcatccagct
     5401 cccggcgaaa ctgctcgtat ttacggagca gaatgccaag acctaattct gttgcagcat
     5461 aaacattatt ttctcctggc gcggaaatcg cattacggat tttttcaaaa gcataactga
     5521 aatcatttct ctcattgaat gcagtaaaca tgtcctttcc tcagttgaat agttagccgt
     5581 gcaattttac cacacctcaa tcatcgccag atgagtgcca ctctgttgag aacagtgact
     5641 ttcacaaaaa atgaatttca catactgaaa taataagtag ccatatagaa acgcagttaa
     5701 attaccggag agccgtttcc gtcagatccg actttccgga atatcacaac tttccggaag
     5761 tgtaccacgc tgagaaaata atcatatcgc tgttttccgc ccctgcgcgg cgcatcacag
     5821 gtcagggaga tgacacaaca tatagtgtca tgagcagctg aatgagatac cacatgttgt
     5881 gctgacagga ggtggcgttg tgtgggggga gaggaagatc cgtaacctgg ggttttcgga
     5941 tctccggaga aataagaaga ggcaggcatt gttgtagcat taaaatcctc aattttagaa
     6001 gcatcacaat tttcaaaaac taaaaaactg attaatagct aataataaaa tgctaggtta
     6061 gcattaagaa caaaacaaac attaaaggat cgatgatgct aagccagctt aacctgcgtt
     6121 ttcacaaaaa acttatcgag gcgctgaaaa cgcgtgccgg tcgggaaaat acttcggtca
     6181 acgccctagc cgaacgcttc ctcgatgacg gcctgaaaac ggtcgcgccc ggtgacgggt
     6241 attttcagct cattgccgat ccggaggcca cggtccggca gctgtaccgg catatcattc
     6301 tgggccagac cttcggcacg tcagcgctct cccgcgacga actgcgcttt gtcctggttc
     6361 acgtcaggga ggccttcctg cgcgggcata ataggctggc cacactcccg gccctggaca
     6421 cgctgctgga catcactggc aacctgctgg cctggcaggt ggagcacgat cgccctgtgg
     6481 acggtcacta cctgaagggg attttccgcc tggccgggaa aaactggacg gaagagtttg
     6541 aagccttccg ggcagcgctg cgccccgtgg tggatcagat gtatgccgaa cacctcctgc
     6601 gtccgctcga gagcgactgt tttggcctgg cggaggtgcc ggacgcggtg ctggcagaaa
     6661 tcttcaccct gccgcggctg aaagctgttt tcccgttgat gctgcgcggc ctggactgga
     6721 acacagaaca ggcaagaacg ctggcacagg agctgcgccc ggttatttct gccgtcacgg
     6781 aaaccattga ggccggcacg ctgcgcctgg agatccgcgt tgacggccag caccccggag
     6841 aacgcccggg ggcctggtac accacacccc gtctgcacct gctgatcacc ggccaggact
     6901 tcgtggtgcc gtacggctgg gaagcgttat ccgaactgct gggcctgttc acgctgtatg
     6961 cccggcatcc ggaagccctg acgcacggtc accaggggga gcgagtgatg ttctccccgc
     7021 ccggaaacgt cacgccggag ggcttcttcg ggatagacgg cctgcggatt tttatgccgg
     7081 cggaggcctt tgagacgctg gtccgggagc tcgccacgcg gtgtcaagaa ggcccgctgg
     7141 cggaggccct gaccgggctg cgctgcctgt acggagatct gtaatatgaa gatactgagg
     7201 cagctgtgga accagaaagg gcttgatgcg gcagttgaag atgtacctga ggatcgatac
     7261 ggtttcggta atatcgcaga aaatatatcc cgttccatcc ttactcttcc tctggaggct
     7321 tcaaatgttg tcggaataga aggtgcatgg ggatcgggta aaacaagcct ccttaatctg
     7381 atcctgagaa accttgccct gaaaaaagat gctcacacgc atgtgctgca tatttctccc
     7441 tggctgagtg gcggcagtcc ggttgaagcg ctctttcttc cggttgccac ggttatccag
     7501 caggaaatgg aaatacgcta tcccccgaag ggcttcaaaa agctctggcg taaatatctg
     7561 ttgtcaccgg aagctcaaaa agtgattgag tatgctcagg atacttcatc gcgcgtcctt
     7621 ccactggttc aatatatcgg acagttttcc agcattatta actggatagc aggcggaata
     7681 aaggtatttt cagacagccg tctggctgtt gatcagaaaa ccacgacaaa gcttcgggct
     7741 gaaattgcag gacaactggt gagtctggat ctgaagttca ttgttgtcat ggatgatctg
     7801 gaccgactgg agccatccca ggtggcggaa gtgttcaggc ttgtgcgtgc agtagccgat
     7861 ctgccccgct ttacccatat tctctgttat gacaggcaga ttatcactca tgccgttgaa
     7921 catgcgctga atatcgaaga tggcagccgt tatctccaga aaatcattca gcttagtttt
     7981 aaattacccc gacctgaagc ctttgattta cgtaatgaat ttcgccagcg ggctgaggct
     8041 ctatatcagc aaattaataa tcaaccgcca gactctggaa tggtaaggga tctcatcgcg
     8101 gtgactgata cctatggtgc cgcactttcg acgccacggg aaatccatca ggccattaat
     8161 tccctgattt ttctttatcc ggggatgcgg gattttgttt atttccctga tttgtgcctg
     8221 cttcagctta tacgggtgac aaacccggct ctgtatgact ggacagagca ttacctgaca
     8281 gaacggtccg tgattgaaac cggtcagggt atgctttctg acggagagaa agcagacttc
     8341 cgggaggggc ttatcagatg tatgaagacg ttcagggcat caaatgcaga ctcgtttctg
     8401 acacttgcag actggatccc aggtatcagt ggacataatg atgaatatct gaatcttttt
     8461 gagcccgtca gtgaggattt tcgtcatatc cagacgaccg gtaaacgact cagcagcctg
     8521 acgcactggc gatattattt tgccttctcc tcaccacaga atgttctgcc acctgaattc
     8581 ttcagacagc tttttgaaca ggctggcgta tcggaaaaac agcagcagct ttctgaattg
     8641 ctgctctcaa aaatcaacag tgtgggcagt ctgtccggaa cctggtttga acatatactg
     8701 agcagactga ctccaggcct gataagagaa cgtaatttcg aagagtgcgc aggactggtt
     8761 cattttttct tcgatcacac cgatgaggtg agcactcgct tcagtatccg taacccatgg
     8821 ttttccctgc gtgaaatggc cattaatgag gttgtacggc atctgctgaa gcatatgcag
     8881 gatattgatg agaccagaac gatcacgctt atggaaaaac tgatcgttac gggtgcttcg
     8941 ccgttctgga tagccgattt tatgcgggat cttatctggg aacatggact tgctcagaat
     9001 gcagtacctt cgccttcaga cgctcttttc agtcgcgata ttacggaacg gttgcgtgac
     9061 aggtttgcgg aacgtatgaa tcaaccagaa cttcagcaac agcttctcct gcgcaagtct
     9121 cttctcgggt atctctacgc ctggagagac atgagttcag gtgaaaccgt gaagcaatgg
     9181 gtcagagaag ttactaccac tgatgaaggc cttgttaacc tgttgatacg attacagacc
     9241 agtgtcttca gctcccaccg aggggcatat cgccggattg cgcgtgacca ggtcagtccg
     9301 ttctttgatg actggccagc agtcgaagag aagttgaagg ttatgctgtc cggcaacgag
     9361 ctgactccgg agcaggaagc gctcaaaaca gcgctggaaa atgacgactg accaaaaatt
     9421 aaggccgctt agcggccttt ttcttactaa atgaaggaga tcaggcgctt aatgctttat
     9481 ccttgtcaaa aggaacaccg gcatacgcaa gctcactcag cagtttgcgt ttccatccac
     9541 catgctcatc aagctcggta tagagaaccc cggcaagatc tgaagggatt tcgatatctc
     9601 ccttcctgag tacaagtact ttcccgcgtc caagcttccc ggcgaaatat ccgagctcat
     9661 acaggacatt ctggcgtgcg cggagacgtg tgctttcaga tgccagagca ctgccgctat
     9721 cgtcgggcgt catcagcacg atggcaaaac tgacatcccc tgactctgct tcaaatttct
     9781 cgataatcgt cctggagccg tcaggacgtt ctgaaagaat gatttcctca agacctatcc
     9841 gggatataaa acgggcaaca gactgaaggg catcattatc atggccatga acaataaaaa
     9901 tttttcggga aaccggccga aacactgcag gctcaggatc gtgttggttt tccacataca
     9961 actggtaatc gcgcacgaac ggggcaacta atgacgacgt gaattttcga atggattcaa
    10021 taatgtttcg atcatagaaa aatgtatgac agaaattaaa ggaaaaattg ttatcgtctg
    10081 ccattttctc gatcagcagc aacttcaggc ccaaaatatc agcgggctca tcaggccact
    10141 gtagaacagc ggaaccagcc atgctggatt cagtatcttc actcctggcc agaaaggtat
    10201 caagttcaac attgcgggtc agatgtgcat taactgactg cagattctct gagttcaaga
    10261 gccgggccaa ttgcttaaga ggttgctgat agttctgaac gtcagcgcgc tgaaggtcga
    10321 atacgcaatt tttaagtgac tgaaagatat caattgccat cctgcccgct ctcctgtctt
    10381 tgttgttctg ctctgtccag aatctgttgg cgcagatttt ctttcatgtg ttcgattaat
    10441 gggctgacca tcggattagt ggcataacca gaagcgcgtt cgtacaccat ggaataggct
    10501 tcctgaatcg cgttctcaat gctgacagga tcgttagcat cgaagtggac atcataactt
    10561 ccgtttaatg cctctgttgc tctctcaacc tcttttagct gtttctgcat tttgtctaaa
    10621 cctgtgattt tgacgctcat aacaacctcc ggactaagtg agatgtatca acctttatag
    10681 atagcacgtt ggagatgtgg ggtaagggat atcactcaaa ttccatgtaa tttcattaag
    10741 atcagactta accttttttc ggatctccga taaaaaagaa agaaatagag atatgctaaa
    10801 catatgcctg gcatctacat atgctaccgc tatacatatg ccacacatct gcctatacta
    10861 aacatatgca cacacccggt gctttgaata ttccggatat gtttcacata tccatatgct
    10921 cgggagagaa aaaacatgag aaccgtatcg atattcaaaa atggcaacaa ccgcgccatc
    10981 cgtctgcctc gtgatctgga ttttgagggg gtgagcgagc tcgagatcgt ccgggaaggg
    11041 gacagcatca tcctgcgccc cgtccggcca acctggggat cattcctaga gtacgaaaaa
    11101 gccgatccag attttatggc ggagcgcgag gatgttgtca gcgacgaagg gcgatttaac
    11161 ctgtgaacaa gacttatatg cttgatacct gtatctgctc gttcatcatg cgtgaacagc
    11221 cagaagccgt cctgaagcgt ctggagcagg cggtgctacg cggccaccgt atagtggtct
    11281 cggccatcac ctactctgaa atgcgcttcg gggctaccgg cccaaaggcc tcaccacgtc
    11341 acgtccaact ggtcgatgaa ttctgcgccc gcctcgatgc catcctgccc tgggatcgcg
    11401 ccgcagtaga cgccaccacg aagattaaag tggcactgcg gctggccggg acgccgatcg
    11461 gcccgaacga cacggcgatt gccgggcacg ccatcgccgc cggggcgata ctggtaacga
    11521 ataatacaag agaatttgag cgagtgcctg acctggtgct ggaagactgg gtgaagtaag
    11581 acactctttt caaagtcagg agataagggc accaagaaac agcccgtata cgggctgttt
    11641 taacagatat ctacagtaag cggtcctaaa ctgtagggta tcttcgaaga tcagactatc
    11701 agggagctaa agcctatctt ccggcggcaa aaacagtggc tcaagcgata cgggcaaccg
    11761 agctctggtt tgctgccggt ggcagggcga tgagcttgtc acattccata atcacgtact
    11821 ccgggatgag atcgaatctg gacggatcta gttgacagat gaactcattt tcaatgtggt
    11881 acgtttcgtt aacgaatttt cgtattaccg gatggtcctg atccagttcc agtaaacgga
    11941 atacctgcag tttttcatag ccgttacgac agcgacggta caggtttttt atttcatctg
    12001 ggctgctgac gagagcctga agtcgcgggt aatcaaaact gtccacatac tcccggatat
    12061 cccgcaaggc ctgctgaatt ttctcaggag ccattttggg atagcccgag cccgcagcgg
    12121 caggctcgcg ataatccagc gggacgacgc gtcgatggaa tagattggat agaagctgat
    12181 aagcatcacc acgttcgtca acgatttcaa agtagcgacg aagatagatg agcttaatga
    12241 tctcctcaca gtctgctgac gcggtgatgg atttgcagat ctgcatgaat gtcatgatat
    12301 cgccgtcgtt aacaggtaat tcttctatga ctccagcaga caggcgcagg caggaggcgg
    12361 tcacctgatt actgaacaac cttctgactg acttcagcgt gtcaatcaca ggctccacgt
    12421 catgcgtcag cataagtaca gtccggttct tcaggcattc accacttgca cgtctgaaca
    12481 gcatttcaag aatggcaaac ttcttgtttt tatcaaaaga agatatggga tcatcaagga
    12541 taatcagtcc cgggtttttt gacaggcatt catacatgaa taacacaatc gcaaaggcgt
    12601 tccgttctcc atagctgaga tgctggctac cgccgctgac gtacccgtca aaatctatgt
    12661 gtcgtagcct gagttttctc tgctctccct cgccggctat atccaccctg tatttatagc
    12721 ctgcataagt gaggaaatta ttgatgtttg ttttgtgctg tgcgatcagc cggatcatgc
    12781 tgtcccggtg ccggttaatt tttccctgca gcggtccggc aaggtttatc aggtccataa
    12841 gggccgcgtt cagtctgtcg gtgatcccct gcatgagttc ggattgcaga tcagggaaga
    12901 actgcaggtc aatcaacctg gcagtgagca cctcccgaac gttctgctgt tcctgcaggc
    12961 tgaaaacatt caggcctctc agcgcagtga gtttctctgt cagcgtatcg gtctggcgtt
    13021 tcaacgccac taggtactcg atatgttcgg cttccggacc gttctgaagc atagtaatgg
    13081 ccagaaggcg ttctctggcg ctctccgtca ggtaattacc gaggttttcc accagtctga
    13141 tgatagcggt caggttttta atggtggatt tatcgtattc ctctctgact tggcgaattt
    13201 gtgcttcctt tcccgtgata tcaccggtac agaacggaca gcagccatcc gaaaggggcg
    13261 aaaactcgag tccctttgtc tgccagtcaa tccattccac gcggcgttca ctgcggatgt
    13321 agggttgata gttctcaaga ccagccggaa tgtggtgtat tttatttccg cctgacagac
    13381 ctctcatgcc ggttgaagaa cgtgaaatcc cggaactggt tgatctgaaa gcattactga
    13441 gttcctgtag atggtctatc agagagttca gctcagtgtg atccgtgaaa acagcccgga
    13501 tcttttgcgt catttcttct atttcacgct ctctttccgc atgggcttga ttgcggataa
    13561 gaatattaaa gctatcactg atgagctcgt ccggttggaa cgtaaactga ctgacgtact
    13621 cctcgttgaa gcacatcacg tccccaatcc cgtcagcacc gatgacaatg ggcccggtgc
    13681 tatcggggtt ttctccgcgc agccggaagg gcatcagtgc ctgtagtccc tgaatgtcgt
    13741 cccgcgccgc acagcttatg gcccgtgaca gcgtactttt tccagtacca tttggcgcaa
    13801 acttaatgtt cagtttatcg gcagtgaggg tgatatgtgc tcgtacgatg ttattgcagt
    13861 ggcgtatttc aatgtccatt tatctgtcct ttgccagtta ttccgggggg attcatatcc
    13921 ttccgcaaaa tgtgcccgca tgctgtgatg cagatacata atacatttcg tttataccat
    13981 tgcttacacc tctccgaggt gttcatatca gaaacaaaga ttaggtcgat ccgacttagc
    14041 cggaaactgg atctgcgcgg cagaaaagac gcaaatgata tgataattaa gaaatatata
    14101 taaaatcata tcagatatgg ttttcatcat tgatgataaa acaatatctg atattatcag
    14161 aaatcatgag cacacaaatc atgcaggata ttatttttac ctgtcagtac attatcagat
    14221 ggagcgtgca ggatggatga agaaagggtg ttttcactca gctatgaaca actgacccgg
    14281 tttgcggaaa aacgcatccg ggaatgcaat ctggacagcc agggcgcgat ttatctctgc
    14341 gagtcagcca aagccggagc cgtgctgatc ttctggcatg aactggccat aaatggctat
    14401 gcctcaatga atgccataaa gagacaggag ctcattgacg ccgactttca gcgcctcaga
    14461 aacctcatct ggccggagga tgacaggtaa tgacccagag ccgccgaccg tcgccactgc
    14521 agcggagagt gctgattgtg ctggctgcgc ttgatgaaaa acggccgggc cccgtgctga
    14581 cgcgggacct ggaacgggtg ctggagcgaa gcggggaagc cccggtctac gggccaaacc
    14641 tgcgcgcctc ctgccgtcgt ctggaggatg ccggctggct gcgtaccctg cgcgccccga
    14701 atctgcagct ggctgtcgag ctgacggacg ccggccgggc cgtggcacag cctctgctgc
    14761 tggcagaaca ggaccgcctc cgggcagaac agcgtgcggc agaagtcgtc gtactgccgc
    14821 tggtcccggc agcaggtctg cccgcagacg gcacttctgc cactgatctg gcggtgcagc
    14881 tgaacggcat aacgtatcag gcctgccgtg gggattttgt ggtacgtctc gacggcagca
    14941 cctgtctgca gttatggaat aaagaaggca gggtggtacg cctggagggc gatccgctgg
    15001 aagtggcgca gtggctgcag gcctgtcacg atgccggaat ggaggttcgt gtacaggtta
    15061 atgaaagcgt caccccgtaa aaacatacca gcacggttta cacatcatcc catggtggca
    15121 gctggttaac tttcctgcgc caccagtgga taggagatat gatcagtcat aacattaagt
    15181 ctaaaattac agacatctca aaaagtctgt agcataaaag aggcctgatg tagaatactg
    15241 tgccaaatag aatattaaaa ggatttgata tatcccctgt tcacagttat atgtttctga
    15301 attacatggt gaatcccccc aacataatgc taggatttga aaatggcaga aaaaaataaa
    15361 aaaaccatta ctggtcaagt cttgaactcc attaaaatta acaaactaaa atgtataaat
    15421 ggattaaacg agattatttt taaacctcat gcgttaaccg caattttagg tccaaatgga
    15481 agcggaaaat caacaatact ccatgctata gcaagcatat acatgcctga agaaggtttc
    15541 cccggtgaag accatcggtt aatgcatttt tttccacgca gcccacatgc tgaatggaac
    15601 ggcagtgact tcattgtaaa tttaacttat cgtaaagatg gggtaatgat tgaaaatgaa
    15661 ttgaaaaatt atggaaaagc agatattcga ggctcacgat ggattcagat ttatgctcgt
    15721 cgtcctttaa gagaagttta ctatcttggc attgataaat gtgttcctat aatagaatca
    15781 gaaaaaaaga ataatattca gtatgaaacc agcagtgtca gtaatgattt aataacaaac
    15841 attcttcatt acgcctccta tatacttaat aaaccataca caagtttcaa tcagcatcaa
    15901 caacccaatg gaaaaatatt gattggagtt gagtcagggg gactggccta ctcatcattg
    15961 agtatgagcg caggagagca aaagatattt ttaattctgg aaacaatatt gaaagcggac
    16021 aagaacgcct taatattaat tgatgaatta gatttattgt tgcatgacga agcattgaaa
    16081 aagttaatag aagtcatatc atctcatgcc aaagataaaa acaaacaaat catctttact
    16141 acccatagag aaatgataac cactttatca gacaagataa atataaggca cgtcgtaaac
    16201 attcaaggcc gtagttattc atttgaagaa acaaaacctg atgctataaa cagattaaca
    16261 ggtgaatcaa cgacgcctat tgaaatatat gtagaagatg acttagctgt tgccataata
    16321 aataaaatat gttcttcact taaggccagc aaatatgtaa aaatatttaa atttggcgca
    16381 gcttcaaacg cattcactct acttgccagt acccttattc gtggtgacaa tctatctggt
    16441 aaactttata ttcttgatgg cgataaatat tccacagaaa atgaaaagaa aactgctctt
    16501 gataaagttt tcaccggcac agaatcacga acttatgagt taaaagcggc agcggaagga
    16561 aaagtaaaac aatttaatct cccaaatggg gttaagcctg aacaatacat tcattatctt
    16621 attacaaatg tcccactgga cggattgggt ggcgaatatt tagaaataat cgaagcagca
    16681 agggatatca gagttgaact tgatgctcat aattatatct cgaatatttt aactaaattg
    16741 ggcattgatc gtccgtccgg gttaacacga gtgatggatc tggcctccag gcatcccgaa
    16801 tggcatcagt atgtgagtga agtcactgac tggttacagc ccgtagtttc tgatttaatg
    16861 gaacgattac ctgagaatga tacagtagat ataacttaaa atatccaacc atcccgaatg
    16921 agcaaataca aaaactcatt tgggataata attaatataa ttatgctggc tcatttcatg
    16981 cctgtcaaaa aaaaacaaat tttgctctgt tgttttctta acgaagatat tatgttaatc
    17041 gtgaaaatat gatatatatt attatataag aacacatcgt agaggttttt atggggaaat
    17101 atcaggaaga aaagaaaaca cccatcatta tggtgaaaaa acgtcgcact cttttacccc
    17161 cttccttatc tgaaaaaaca ggtgttatcg cacgttcaga gacagaaaag atggaagaat
    17221 cagtctctga ggggatcagt tcgcctgccg tcgatactca tacccctgat gctcctgcct
    17281 gtaagaaaaa aaagaaaaga cgccgttttc cacgccagcc tcattggact catgaattta
    17341 ctcacgattg cgtggaaaaa gtgaagtctc tgtttcccca tcttcgcgca gaaggcggag
    17401 gatttcttcc actgaaaatt ggcataacca atgattttgc cgcattcctg acagaacacc
    17461 ctgagacaga actgacactg gatgaatggt cctgtgcaat atcctgcatc accacccgac
    17521 aggtatatct gcagcgtact gctgtcgcag gtatccctcg ttatggactg gatggactac
    17581 cagcaggtca ggtatcagaa tgcgatgcgc tgaatgccag acgctggctg gcagtaaggg
    17641 aacaacagaa gctaaagatg aaaaccatgc aggaacagtc cgcgtcaacc gaaaaaactg
    17701 aacggtaatt tcatcgcgat aacagcaggt tacaaacaca ataatattca ttgtgaagta
    17761 ttcctttcag atcagcttat atgttttttg caggctgctg gtagaacagt tgcttttact
    17821 tgtggtcagt aagcagagat attccgacat gggcattctt caggctatag ccttacagga
    17881 tcaatcacca cctccctagg aggtggttta gggataacaa taaaagggct acaccgttag
    17941 aaccgcgccg taagactgaa gggattttca tattgacgca aaaatccctt ctcataagga
    18001 ttaaaacaaa aattgcgtta aatatcccat aaggattgcc atacctaaga taaccgtcag
    18061 aaatgcgatt atcatgggca ttctgaacat tgatttaagc aatattactt ctgtcagact
    18121 cgctccagca ctaccgatta tcaaggccat taatgccccc aggcccattc ctttagtcat
    18181 cagcactgat gcaagtggta tcactgcctc tgcgcggata tacaacggaa taccaacaac
    18241 tgcactcagc ggtatggcta agggattatc tgctccggca tgagctgcaa tccactctga
    18301 agggataaag ccataaataa atgaacctat taatacactt aacaaaagat aaggcagaac
    18361 atccttaaac tgctgtaagg catctttcat agctaacttt attgggccag aggtggtatt
    18421 cgttgtgaga ttcacattgc ccagattctc gctgctgcaa cagctaacag ctggttgttc
    18481 agatgatatt gatgaacaac acgctgatac gttctgctcg ttctttaccg tcctttttac
    18541 cgaagcttcg gaatcatcgc atttcgtggt gcaaccactt gatgctgagt ttttgtgatc
    18601 aacaatatga cgttcaaatc ctagtaaatc caatacgata ctggcaagga ctgagacacc
    18661 ggcagcgatg atcgcgtaca acaaagttac tttccagcca aaggtaaccc atattaaccc
    18721 cacgataatg ggattaagca acggtgaaac aaacaaaaaa gtgagtgtcg gaccaaaccc
    18781 cgctttcgca gacaacaatc ctcgcaacat tggaactgtt gagcaactac agaaaggggt
    18841 aacggctccc agcaacgagg ccagcagata ccccttacct tttctcgccc ccatcatctg
    18901 ctgaatttta tgatccggga ctttttgcct tatcaggctc acaccggcac taatgacaat
    18961 aaacagcaat gaaagctcaa cagccagaaa aacaaacatc tctgcggcat cttgcaacat
    19021 aggaatccaa ctattcatac taaacctctt gacagaatct attattctag aataatcgaa
    19081 atataattat agtcaagata tttctggtat tatcgaaata tcatcaggca gagggttgtc
    19141 acatgcaact ggaagaagta gctaaagctc taaaagaact gggtcacccg acccgtttgt
    19201 tcatattcaa acatcttgta aaagctggag aacaaggttt acctgtaggt gaattgcaaa
    19261 agcagcttgg cattccttca tcaacgttaa gtcatcatat ttcagccctg gtatcagtag
    19321 gactcgtcac acaaaaccgg gagagtcgaa cgctcatgtg cgtctctcaa tatgaaatac
    19381 tggaagctat catcgaattc ttacgtgaag agtgctgtgt aaacagtaaa acagatgttg
    19441 cggaacccgc tggcaaaaat gggtaagtaa ccggaaaact cataactgtc atgattgacg
    19501 aatgctgtat ctacccgtgc gaagcacacc taattaagcc aagaatatat agagaaaatc
    19561 ataaaaatta ataggttaaa taatatagtt cctgattta
//
LOCUS       DACAPB010000075        19572 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.075, whole genome shotgun sequence.
ACCESSION   DACAPB010000075 DACAPB010000000
VERSION     DACAPB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.075"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            400..963
                     /locus_tag="IT904_002409"
     CDS             400..963
                     /locus_tag="IT904_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3192179.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSANKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            1045..3687
                     /gene="yfcU"
                     /locus_tag="IT904_002410"
     CDS             1045..3687
                     /gene="yfcU"
                     /locus_tag="IT904_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HAP3192180.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGVEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWSDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFTVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVTSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            3707..4459
                     /locus_tag="IT904_002411"
     CDS             3707..4459
                     /locus_tag="IT904_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP3192181.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNNCQVDEEQSRKG"
     gene            4475..4984
                     /locus_tag="IT904_002412"
     CDS             4475..4984
                     /locus_tag="IT904_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192182.1"
                     /translation="MSHKFLAGAGLLVLAGWGVSGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVEFGNVVINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            4981..5469
                     /locus_tag="IT904_002413"
     CDS             4981..5469
                     /locus_tag="IT904_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192183.1"
                     /translation="MKKFLLLLLCAGCGVVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQEVDYELSCESDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            5466..6005
                     /locus_tag="IT904_002414"
     CDS             5466..6005
                     /locus_tag="IT904_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192184.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLIVAGQSVHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGEFTASLTATFEYI"
     gene            6007..6864
                     /locus_tag="IT904_002415"
     CDS             6007..6864
                     /locus_tag="IT904_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAP3192185.1"
                     /translation="MKILRYVFYFLLLICLSTGVQAAKKIATLYIPAGSMGVLTGFQF
                     NLSILTPEGAQYGVYQSRSVAILNVPLVSWTGQGTAPELHLEDARTILPTSRCPGLEE
                     YDARTPQTTWACVEMKVTVFYDGDMHGCPWIVSSYVKSEDATEAIYTGNNVPYIGPTK
                     VSSDCSAIPLDPYDVSWNENTVVHNKIVRMQSTGAVIEQTLPTYLMEGGKLCDGSKYD
                     ERGAYCRFVAQQMTFSTSGCDDAKVTVTPEPQPITSRQLHDMKLRVDTTAQQPIDATC
                     RFTYILNMY"
     gene            complement(6936..7487)
                     /gene="smrB"
                     /locus_tag="IT904_002416"
     CDS             complement(6936..7487)
                     /gene="smrB"
                     /locus_tag="IT904_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAP3192186.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7653..8585
                     /gene="prmB"
                     /locus_tag="IT904_002417"
     CDS             7653..8585
                     /gene="prmB"
                     /locus_tag="IT904_002417"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP3192187.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8620..9705
                     /gene="aroC"
                     /locus_tag="IT904_002418"
     CDS             8620..9705
                     /gene="aroC"
                     /locus_tag="IT904_002418"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP3192188.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLDIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9709..10533
                     /gene="mepA"
                     /locus_tag="IT904_002419"
     CDS             9709..10533
                     /gene="mepA"
                     /locus_tag="IT904_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAP3192189.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCFDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10533..11342
                     /locus_tag="IT904_002420"
     CDS             10533..11342
                     /locus_tag="IT904_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAP3192190.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11342..11890
                     /locus_tag="IT904_002421"
     CDS             11342..11890
                     /locus_tag="IT904_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAP3192191.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11924..12202
                     /locus_tag="IT904_002422"
     CDS             11924..12202
                     /locus_tag="IT904_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAP3192192.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12323..14329)
                     /gene="mnmC"
                     /locus_tag="IT904_002423"
     CDS             complement(12323..14329)
                     /gene="mnmC"
                     /locus_tag="IT904_002423"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAP3192193.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQARLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDMAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14488..15708
                     /gene="fabB"
                     /locus_tag="IT904_002424"
     CDS             14488..15708
                     /gene="fabB"
                     /locus_tag="IT904_002424"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAP3192194.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15973..17151
                     /locus_tag="IT904_002425"
     CDS             15973..17151
                     /locus_tag="IT904_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192195.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLAYGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17148..18143)
                     /locus_tag="IT904_002426"
     CDS             complement(17148..18143)
                     /locus_tag="IT904_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAP3192196.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQKKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18242..19378
                     /gene="pdxB"
                     /locus_tag="IT904_002427"
     CDS             18242..19378
                     /gene="pdxB"
                     /locus_tag="IT904_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAP3192197.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19444..>19572
                     /locus_tag="IT904_002428"
     CDS             19444..>19572
                     /locus_tag="IT904_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP3192198.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESA"
BASE COUNT         4603 a         5018 c         5251 g         4700 t
ORIGIN      
        1 ttctggctat agtgtttata tattatattg tcaaaagtgg ataaatgcca aacgcattat
       61 atgttcattg tttgtttttt aagctgttga tattcattat tgatatactt tctatttttt
      121 ctaaatactt attccgtatt ttttaaggat gtccttaaga taacctttct tctgtcaata
      181 tattaattaa atctacattt actctttagt aaaattaaat tgcatcaggg gtgtggaaac
      241 atcactatgc ggccccacac caacgaatac aattttctac gacctgttct ttttggatcg
      301 catgccgtat tcaatggctg ggtataaaca ttggctattg actgaatcaa tgtattgcaa
      361 tgtttatatt tttaattatt tatagtttaa ggacaaataa tgagtaagtt tgttaaaaca
      421 gctattgctg cggcaatggt gatgggcgtg ttcacctcta cggcaacaat cgctgcgggt
      481 aacaacggta ctgcacgttt ttacggcacc attgaagact cagtgtgttc tatcgttccg
      541 gacgatcaca aactggaagt tgatatgggt gatatcggtg ccgaaaaact gaaaaataac
      601 ggcaccacca cgccgaaaag tttccagatt cgtctgcaag attgcgtatt tgatactcag
      661 gaaacaatga ccactacctt tactggtacc gtttcttctg caaatagcgg caattattac
      721 accattttca ataccgatac cggtgcggca tttaacaatg ttagcctggc gatcggtgac
      781 tctctgggta cctcttacaa aagcggcatg ggtattgacc agaaaatagt gaaagacact
      841 tctgctaata aaggcaaagc gaagcagaca ctgaacttta aagcctggct ggtcggcgca
      901 gctgatgcgc cagatctggg taatttcgaa gcaaatacca ccttccagat tacttacctg
      961 taaggcatac ccccatagcg acactgctat gggggattta aatggatttt gaccgaacaa
     1021 gaataaaaac ggatagaaac gtgtatgcct gatcattctc tttttcgatt acggattctt
     1081 ccgtggtgca ttgcgctggc aatgtcaggg agttatagca gtgtctgggc tgaagacgac
     1141 attcagtttg attcccgttt tctggaatta aaaggcgaca caaaaattga tctgaagcgt
     1201 ttttccagcc agggatatgt tgagcccgga aaatacaatt tacaggttca actaaataaa
     1261 cagccattgg cggaagagta cgatatttac tggtatgctg gtgaagatga cgcgagcaaa
     1321 agctatgctt gtctgacacc ggaactggta gcgcagtttg gtttaaaaga agacgtggcg
     1381 aaaaatctgc aatggagcca cgatgctaaa tgcctgaaat ccggtcaact ggaaggcgtg
     1441 gaaattaagg ctgatttaag ccagtccgca ttagtcattt cactgccaca ggcttacctc
     1501 gaatatactt atcccgactg ggatccgcct tcacgttggg atgacggcat ctccgggatc
     1561 gtcgcggact acagcatcaa cgcacaaacc cggcacgaag aaaatggcgg tgatgatagt
     1621 aacgagatca gcggcaacgg gacggtcggg gttaacctgg ggccgtggcg tatgcgtgct
     1681 gactggcaga ctaactatca acatactcgc agtaatgatg acgatgaatt cagcggcgat
     1741 gaaactcaaa aaaaatggga gtggagtcgc tactatgcct ggcgggcgtt accatcatta
     1801 aaagccaaac tggcgctggg cgaggattac ctcagatccg atatttttga tggttttaac
     1861 tatgttggtg gcagtgtcag tactgacgat caaatgttgc ctcccaatct gcgcggctac
     1921 gcgccagaca tttccggcgt ggcacacacc acagcaaaag tgaccgtcag ccagatgggg
     1981 cgtgtgattt acgaaacgca ggttccggct ggaccgtttc gtattcagga tcttggtgat
     2041 tctgtctccg gtacgttgca tattcgcatt gaagaacaga acggccaggt gcaggaatat
     2101 gacatcagca ccgcctcgat gccatacctc actcgtccag gtcaggttcg ctataagatc
     2161 atgatgggcc gtccgcaaga gtggggacac catgtcgagg gtgaattttt ttctggtgct
     2221 gaagcttcct gggggatcgc taacggctgg tcgttatatg gcggcgcact gggagatgaa
     2281 aactatcagt ctgcggcgct tggcgtcggt cgcgatttgt ctacattcgg cgcggtcgcg
     2341 tttgatgtta ctcactcgca caccaaactg gataaagaca ccgcttatgg caaaggttcg
     2401 ctggacggta actccttccg tgtgagttat tccaaagact ttgaccagct caacagccgc
     2461 gttacttttg ctggatatcg cttctcggaa gagaacttta tgaccatgag cgagtatctg
     2521 gatgccagtg acagcggaat ggtacgcacg ggcaacgaca aagagatgta caccgccact
     2581 tataaccaga acttccgcga tgcgggtgtt tcggtttatc tcaactatac ccgccatacc
     2641 tactgggatc gcgaggagca gacaaactac aacatcatgc tctcgcacta tttcaatatg
     2701 ggcagtattc gtaatgtcag catctcgatg actggctacc gttacgagta tgacaaccag
     2761 gccgacaaag gcatgtacat ttcgctcagt atgccgtgga gcgacaacag taccgttagc
     2821 tataacggca actatggcag tgggacggac agcagtcagg tcggttattt cagccgtgtc
     2881 gatgacgcga ctcactatca gttgaacgtc ggcaccagtg acaaacacac cagcgttgac
     2941 ggctattaca gccatgatgg ttcgctggcg caggttgacc tcagcgcgaa ctaccatgaa
     3001 gggcaataca cctctgcggg cttgtcgtta cagggcggcg caacgcttac tgcccacggt
     3061 ggcgcacttc accgtaccca gaatatgggc gggacacgct tgttgattga tgccgatggc
     3121 gttgccgatg ttccggtgga aggtaacggg gcagctgttt ataccaatat gtttggtaag
     3181 gccgtcgttt ctgacgtcaa taactattac cgcaatcagg cgtatatcga cctcaacaaa
     3241 ctgccggaaa acgccgaagc aacccagtcg gtggtacaag ccacgctaac tgaaggggcg
     3301 attggctacc gcaaatttac cgtcatcagt ggtcaaaaag cgatggcggt gctgcgcctg
     3361 agcgacggca gccatcctcc gtttggcgca gaagtaaaaa atgataacga gcagacagtg
     3421 ggccttgtcg atgatgacgg caatgtttat ctggcagggg tgaaacctgg cgaacacatg
     3481 agtgtgttct ggagtggtgt tgcgcattgc gatatcaacc tgccggaccc gctgcctgcc
     3541 gatctgttta acggcttgtt actgccatgc cagcataaag gcaatgtagc acctgtcact
     3601 tcgccggcgg tcaaaccggc gattcaggaa cagacacagc gggtgacgcc aacggaaccc
     3661 ccgacttcaa tttcagtaaa ccagtaacgt gattaaggaa tgatccatgt ttaatctgac
     3721 taacactgca aaaatcgttg tcccggcact ggcgctgctg gcgacagcgg tcagtttctc
     3781 cagccacgct tcggtgacgc ccgacaggac acggctggtg tttaacgaaa gcgacaagtc
     3841 catcagcgtc acgctgcgca acaacgatcc caaactgcct tatctggcgc agagctggat
     3901 agaggatgca aagggcaaca aaatcagctc gccgcttgcg gtgcttccgc cagtgcagcg
     3961 catcgatgcg atgatgaacg gccaggtgaa ggtgcagggg ctgccggata tcaacaaact
     4021 gccggccgac cgcgagagcg tgttctattt caacgtgcgc gaaattccgc cgaaatcaaa
     4081 taaaccgaac acactgcaga ttgcgctgca gacgcgtatc aaactgttct ggcgaccgaa
     4141 ggcgctggag aacaccagca tgaaaagtcc gtggcagcat aaggtgacgc tcatccgtaa
     4201 cggccaggac ttcaccgtca ataacccgac gccgtactac gtgatcatca gcaacgccag
     4261 cgctaaaaaa ggcggcaacc cgccggcggg attcacaccg ctgatgatgg agccgaaaac
     4321 ctccgcaccg ctgaatgcga agatggacag cgtgccggtg ctgacctaca tcaacgacta
     4381 cggcgcgcgg atgccgctgt acttcaaatg caccggcaac aactgccagg tggatgaaga
     4441 gcagagccgc aaaggctgat gccgggaggg acttatgagt cacaagtttt tagcgggcgc
     4501 ggggctactg gtactggccg gatggggtgt cagcggttta gccgttgctg gcagcgatat
     4561 caaacaggtg gatctgacgc tgcgaatcct ggtcgatggc ccgccgccgt gctccgtgaa
     4621 gggaagcgcg gtggaatttg gcaatgtggt gatcaacagg atcgatggta ccaattacca
     4681 acaggatgcg aaatacaccc tcaactgcgg taataaagtc agcgatgact tacggatgca
     4741 gctcaaaggc aataccgccg tgatcaacgg agaaacggtt ctcagcacgg atatcgccgg
     4801 cctgggggtg cggatagaaa acgccgccga caactccctg tttgcagttg gtgaaaataa
     4861 ctggacgcct tttaatgtca acacacagcc tcagctgaaa gcggtgccgg taaaacagag
     4921 cggggtgcaa ctgacgccgt cggagtttaa cgccacgatg accatggtgg tggattacca
     4981 atgaaaaaat tcctgctcct tttactgtgc gctggttgcg gggttgtgct ggcggcggaa
     5041 gatgacatca cattccacgg tacgctgatg tcgccgcccg cctgtagcat cagcggcggg
     5101 caaaccatcg aagtgcaatt tcgcgatctc attatcgaca gcattgacgg caattatggt
     5161 cgtcaggaag tggactacga actgagctgt gagtctgatg tccgcgatcc cgagtggaat
     5221 atgacactct catggacggg gactgccacc tcctacaacg actctgctat tgagacggat
     5281 gtaccgggct tcggaattga gcttcagcat gacggtcagc gttttaagct caacgagccg
     5341 ctttccatta acgccaccga ttttagccag aagccaaagc tggaggcggt accggtcaaa
     5401 gcggctgatg cggtgctgac ggacacgacc ttttctgctt acgcaacctt acgggtggat
     5461 taccaatgaa aacagcacac atttattcac ccggttatgc gctgttagca ggcctgctgc
     5521 tgaccgccag cacgggggca cgggcagtcg ataacaacct gcatttttac ggcaacctgc
     5581 tgagtaaatc ctgcacgctg gtggtggatg gtgccaatct ggcagaaatc cattttccca
     5641 ccgtcagcaa ccgggatttg atagtcgccg gacagtctgt gcatctgccg gttgtgttta
     5701 agctgaaaga ctgcaaaggg ccagcgggct acaacgtcaa agtcacgctg agcggcaccg
     5761 aagacagcgg tcagccgggt tttctggcag tcgatgacac ctccaccgcg cagggcgtgg
     5821 ggatagggat ggaaacgctc gacggcaagc gggtggatat taacaacact accggggcga
     5881 cttttgcgct taccaacggc aacaatgata ttaatttccg ggcctggatc caggcgaaaa
     5941 gcggtcgcga tgtgacgatc ggtgaattca ctgccagcct gaccgctacc ttcgagtata
     6001 tttaaaatga aaattctacg ttatgtcttt tactttttgc tgctcatttg cctcagcaca
     6061 ggagtgcagg ccgcgaaaaa gatcgcgacg ttatacattc ctgcgggatc aatgggagtg
     6121 ctcacaggtt ttcagtttaa tctttctatc ctcacaccag aaggcgcgca atatggggta
     6181 tatcaatcca ggtcagttgc gattttgaat gtgccactag tatcatggac tggtcaaggt
     6241 actgctccgg aactgcattt agaagacgca agaaccatat taccaacttc acgttgcccg
     6301 ggccttgaag aatatgatgc tcgcacccca caaacgacct gggcctgcgt ggaaatgaaa
     6361 gtcacagttt tctatgacgg tgatatgcat ggttgtccgt ggatagtttc ctcgtatgta
     6421 aagagtgagg acgcaacgga ggcaatatat acaggtaata atgttcccta tattggcccg
     6481 acaaaagtca gttctgactg tagcgcaata cctttagatc cttatgatgt ctcctggaat
     6541 gaaaatacgg tcgtgcataa caaaatagtc aggatgcaat caacaggtgc tgtgattgaa
     6601 caaacgctgc ccacttatct gatggaaggt ggaaaactgt gtgatggtag taaatatgac
     6661 gagcgtggtg cttactgccg ttttgtcgca cagcagatga ccttctccac ttcgggctgt
     6721 gacgatgcca aagtgacagt gacgccagag ccgcaaccga tcacgtccag gcaactgcat
     6781 gatatgaagc tacgcgtgga cacaacggcg caacagccaa tagatgccac ctgccggttc
     6841 acttacatac tgaatatgta ctagtgcgtc tttttcacaa ggcagggtca tttgaccctg
     6901 ccattccccg tacatggcgc aacgcgctaa acttctcagg gtaactccgg cggcagccac
     6961 tcttccactt caatcaatac cagcaacgcc gcatcaccac catactcttt tggtgcctga
     7021 tgaaaggcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg cttcaaaata
     7081 tgcttcccgt gcccatgcat cacgcaggcg caaaacacat gttcacggcg gcaggcggca
     7141 atcaacgcgc ccagttcctg cttagcttgc agctgcgtca gaccgtgtaa atccaaaaac
     7201 aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct gacatccggg
     7261 cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc ggaaaaataa
     7321 tggctggcat cagcctgctc ctggatcaag cgtttcaccg gcacttcgct gattttttta
     7381 cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc catcaactgg
     7441 cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta tctgtctcta
     7501 tttctaattt tcctacagtt tacccgactc gtagcgcaat cgatcgtttt aaacgtattt
     7561 ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa actagccgcc
     7621 gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt gatgaagcag
     7681 taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtgagccgc ttcagcgcgg
     7741 cgaatatctg gtacggccac ggcactgaca acccgtggga tgaagccgtg caactggtgt
     7801 tgccttcgct ctacctgccg ttggatatcc cggaagacat gcgcaccgcg cgtctgacct
     7861 ccagcgaaaa acaccgtatt gttgagcgcg tgattcgccg cgtcaacgag cgcattccgg
     7921 tggcctacct gaccaacaaa gcctggttct gtggccatga attttacgtc gatgaacgcg
     7981 tgctggtgcc gcgttcgccg attggtgaat tgatcaacaa taaatttgcc ggacttatca
     8041 gcaagcaacc gcagcatatt ttagatatgt gtactggtag cggctgcatc gccattgcct
     8101 gtgcttatgc cttcccggat gcagaagtcg acgcggtgga tatctctcca gacgcgctgg
     8161 cggttgctga acagaacatc gaagaacacg gtctgatcca caacgtcatt ccgattcgtt
     8221 ccgatctgtt ccgcgacttg ccgaaagtgc agtacgacct gattgtcact aacccgccgt
     8281 atgtcgatgc ggaagatatg tccgacctgc caaacgaata ccgccacgag ccggaactgg
     8341 gcctggcatc tggcactgac ggcctgaaac tgacgcgtcg cattctcggt aacgcggcag
     8401 attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg gtacatctta
     8461 tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc ggcgatggtg
     8521 tgtttatgct caccaaagag cagcttattg ccgcacgaga acatttcgcg atttataaag
     8581 attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa cacaattgga
     8641 caactctttc gcgtaaccac cttcggcgaa tcgcacgggc tggcgctcgg ctgcatcgtc
     8701 gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga cctcgaccgt
     8761 cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca ggtcaaaatt
     8821 ctctccggtg tttttgaagg cgttactacc ggcaccagca ttggcttgtt gatcgaaaac
     8881 actgaccagc gctctcagga ttacagtgcg attaaggacg ttttccgtcc aggccatgcc
     8941 gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg acgttcttcc
     9001 gcccgcgaaa ccgccatgcg cgtggcggca ggagctattg ccaaaaaata tctcgccgag
     9061 aaatttggta ttgaaatccg tggctgcctg acccagatgg gcgacattcc gctggatatc
     9121 aaagactggt cgcaggtcga gcaaaatccg tttttttgcc cggaccccga caaaatcgac
     9181 gcgttagacg agttgatgcg tgcgctgaaa aaagagggcg actccatcgg cgctaaagtc
     9241 accgttgttg ccagtggcgt tcctgccgga cttggcgagc cggtctttga ccgcctggat
     9301 gctgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga aattggcgac
     9361 ggctttgacg tggtggcgct gcgcggcagc cagaaccgcg acgaaatcac caaagacggt
     9421 ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca gcaaatcatt
     9481 gctcatatgg cgctgaaacc gacctccagt atcaccgtgc cggggcgtac cattaaccgc
     9541 tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt cgggatccgc
     9601 gccgtgccga tcgcagaagc gatgctggcg atcgtattaa tggatcacct gttacggcag
     9661 cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat gaacaaaacc
     9721 gcgattgcgc tgctggccct gcttgccagt agcgccagcc tggcagcgac gccgtggcaa
     9781 aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc taatggctgt
     9841 attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat gcgtaccgat
     9901 cagcgtcgct atttcggtca cccggatctg gtgatgttta ttcagcgtct gagtagccag
     9961 gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc cgctggtggg
    10021 cgtttcaacg gcggtcatgc cagccaccag accggactgg atgttgatat cttcttacaa
    10081 ctgccgaaaa cacgctggac ctccgcgcaa cttttgcgcc cgcaagcgtt agatttggtt
    10141 tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt cagcttgatc
    10201 aaactcgccg cccaggacaa agacgtcaca cgcatttttg ttaatccggc gattaaacaa
    10261 caactttgct ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg gccctggttc
    10321 cagcatcgcg cgcatatgca tgtacgatta cgttgtcctg ccgatagtct ggagtgtgaa
    10381 gatcaacctt taccgccacc aggcgatggt tgcggggcag aactgcaaag ctggtttgaa
    10441 cctccaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt gccgccttcc
    10501 tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc ctgtttatgg
    10561 tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga tttatcgact
    10621 cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggcggcg gggatgtctc
    10681 ccgctaatgc gctggcaacc aataaactgc aagcctgcgg cggctctatt tccgccacta
    10741 tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat atcgccatga
    10801 cctttgtcgg ctcaatgagc ggcgcactgc tggtgcaata cgttcaggct gatgtcttac
    10861 gacagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg atgccaaaat
    10921 tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta attgcaggtg
    10981 gctgcgtcgg tttttacgac ggattctttg ggccagctgc cggatcgttt tacgcgctgg
    11041 ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc aaattactca
    11101 acgcaacgtc aaatatcggc ggtttactgc tgtttattct cggtggcaaa gtgatttggg
    11161 cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt tcaagactgg
    11221 tgttgagcaa aggacaaaaa ctgatccgcc cgatgatcgt tatcgtctcg gcggtgatga
    11281 gtgccaaatt actttatgat agccacggac aggagatcct ccactggttg gggatgaact
    11341 aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct gctttgccga
    11401 tgattttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc ctgctgatgc
    11461 ggaagtaccg tataaccgaa tcgtctttgc tcatggcttt tatgccagcg caattcatga
    11521 gatttcgcac tggtgtattg ccgggaaagc gcgccgtgag ctggttgatt tcggctactg
    11581 gtattgcccg gacggacgcg atgcccagac gcaaagtcag tttgaagatg ttgaagtgaa
    11641 gccgcaggcg ctggactggt tgttctgcgt ggcggcggga tatccgttta atgttagctg
    11701 cgacaatctg gaaggggatt tcgaaccgga ccgcgtggtg tttcaacggc gggtacatgc
    11761 gcaggtaatg gattatctgg caaacggcat tcccgaacgt ccggcacgct tcattaaagc
    11821 actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt ggccggaagc
    11881 gctcaactga agccagcgcc acgttttaca atgaggaaag aagatgatcg cggagtttga
    11941 atcacgcatt ctggcattaa tcgacggtat ggttgaccat gccagtgatg atgagttgtt
    12001 tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg aaagtggtga
    12061 tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa aagccattgg
    12121 tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg aaaacctgtt
    12181 tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga tgcgacgctt
    12241 atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc acgccgcatc
    12301 cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc aacaatttcc
    12361 gcacccataa ccgattcggg tttaacgccg ccagcgtact ggcatccatc ggaatcggtt
    12421 cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac aaaccgcgag
    12481 aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc gcctcatctt
    12541 tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga acattgccta
    12601 ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct ttatcactga
    12661 catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc tggcgattct
    12721 gctgctgatc gtcctcactg tacgccatat cttcgctgcc gcgatgataa ctggcaccaa
    12781 tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag cacagcacct
    12841 gcttcagctc tgccaattcc ggcgttgtcg gaatatggct gacctgcccg gcaaccgaat
    12901 acaccgggag agtcgacgtt tggctgaatc ggctgatttg atgcccgttc gccagtacca
    12961 ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag tcatccttac
    13021 gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt tgcgccagtt
    13081 ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga taagtaatgc
    13141 cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca acggctacag
    13201 ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg cttttctcat
    13261 cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt aattggtcgt
    13321 aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc tcatcgtgtt
    13381 tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagt gcgggggcct
    13441 catccgcgca ataaagcgtt acctgccagc cgcgccgtaa tagcgccagc gacaacaacg
    13501 cgctggcaat accaccgccg ataatcgccg cttcccgttt gctgctgccc gtgcggttaa
    13561 accacggcgc ggagcagggg agcggtaatg tctgttccat caccccgcaa agcatttccc
    13621 gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc aaaccgcggc
    13681 ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc aaccttgcca
    13741 tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca aagccgtcca
    13801 gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc agttcgttaa
    13861 tatcgccaaa ccataaatcc agcgtcacgc ggccttcatc gagcaataaa cgatggcaac
    13921 cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc agttccggcc
    13981 agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca aaactaatga
    14041 aatgtaagcg ttgtaatcgc gcttgcggat gtgcttcgcg aaactgatca aatgcctgcc
    14101 aaagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca aacagaggat
    14161 gtggatgctc aggaaagcgt acctctaatt ggttacctcc cagaaaaacg taacgcgtct
    14221 cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa acaggtgtac
    14281 cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg taagttactc
    14341 gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa tttctctatt
    14401 aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt cgaaacttac
    14461 tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg cctgggcatt
    14521 gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga aggacgttca
    14581 gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt ctggggcaac
    14641 gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat gagcgacgca
    14701 tccatttatg cattcctttc tatggagcag gcaatcgctg atgcgggcct ctctccggaa
    14761 gcttaccaga ataacccgcg cgttggcctg attgcaggtt ccggcggcgg ctccccgcgt
    14821 ttccaggtgt tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc ggttggcccg
    14881 tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac cccgtttaaa
    14941 attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca ctgtatcggt
    15001 aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg cggcggcgaa
    15061 gagctgtgct gggaaatggc ttgcgaattc gacgcaatgg gtgcgctgtc tactaaatac
    15121 aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg tttcgttatc
    15181 gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc gcgtggtgct
    15241 cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga catggttgct
    15301 ccgtctggcg aaggcgcagt acgctgcatg aagatggcga tgcatggcgt tgatacccca
    15361 atcgattacc tgaactccca cggtacttcg actccggttg gcgacgtgaa agagctggca
    15421 gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa agccatgacc
    15481 ggtcactctc tgggcgctgc tggcgtacag gaagctatct actctctgct gatgctggaa
    15541 cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc tgcgggtctg
    15601 aacatcgtga ccgaaacgac cgatcgcgaa ctgaccaccg ttatgtctaa cagcttcggc
    15661 ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt cgcagtaggt
    15721 cggagtagac gcgccagcgt cgcatccgac gttacgcgcc aatgcggcct ccggcactaa
    15781 cgcaaaaggg aacctgatgg ttcccttttt cacatcattg acaatcgccg ccagttccag
    15841 gcaaacttcc cgctttgtcg atttccttct gaaaagacgt acgcgttaaa tcctgccaac
    15901 gcaccgtaac cctgaaacca gagagatgag acggggatac tcctcgcctt gcgctgcatt
    15961 ctggagtaat gcatgactgc tgtaagccaa accgaaacac gatcttctgc caatttttcg
    16021 ctcttccgca tcgcttttgc ggtttttctc acctacatga ccgtagggtt gccgttgccg
    16081 gttatcccgc tgtttgttca tcatgaactg ggctatggca ataccatggt cggcattgcc
    16141 gtcgggattc agtttctggc tacggtgctg acgcgtggtt acgccgggcg actggccgat
    16201 caatatggtg caaaacgttc ggcgcttcag ggaatgttag cttatggtct ggctggcggc
    16261 gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt tcaaatttgc tctgttggtc
    16321 gtcgggcgtt tgattcttgg ctttggtgaa agccagttac tgacaggcgc tctgacctgg
    16381 ggactaggca tcgtagggcc aaaacactct ggcaaagtga tgtcatggaa cggaatggcg
    16441 atttacggtg ccctcgctgt tggtgctccg cttggcctgt tgattcatag ccattacggt
    16501 tttgccgcac tggcgatcac cacaatggta ttacccgtac tggcgtgggc ctgtaacggc
    16561 acagtgcgca aagtaccggc cctggcggga gaacgtccat cgctgtggag cgttgtcggg
    16621 cttatctgga aaccagggtt aggtctggca ctacaaggcg ttggttttgc tgttatcggg
    16681 actttcgttt cgctctactt tgccagcaaa ggatgggcga tggcgggctt tactcttacc
    16741 gcgtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg gctggatgcc ggaccgtttt
    16801 ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa cggtgggctt gttgctgctc
    16861 tggcaagccc caggtgcatg ggtcgcatta gcgggcgcgg cgttaaccgg agccggatgt
    16921 tcgcttatct ttcctgcgct gggcgtggag gtggttaaac gcgtcccctc acaagttcgc
    16981 ggcaccgcac tgggcggtta cgccgcgttt caggatatcg ccctcggcgt ctccgggccg
    17041 ctggcgggaa tgctggcgac cacgtttggt tactcttcgg tatttcttgc cggggcgatc
    17101 tctgcggtgc tgggtattat tgtcacgata ctgtcatttc gtcggggtta acgaaccagc
    17161 cagaccagca tcaggataat cgcgactaac agtatccaca gcgcgggacg cgttgccata
    17221 ttttgcaacg tgtccatcat cggtggaaaa ggattaagca gcggttgcat aacacgcgga
    17281 tcaatccctt taacccgccg ttgataaagc tgattaagaa tatcctcggc ctgggcagaa
    17341 gaaagcgatg attgcgcaga aagaccatat tttttctgga tatatgccga taacgccgcc
    17401 agttcactgg catctaaagg ttgttttagc gtcatctgta gtgattccag cgtcggcgta
    17461 ttttgctggc ttagcgtctg acgcgcctgt agccaggtca ccagatggtt aaacagtttc
    17521 gctggaatta actcgccatc tttcaccccg gaaagttcca gcatcgattg ccagatctgt
    17581 ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca gctgttttag cgcattgtgc
    17641 tccgccggta ataaaggacg gtcggtcgcc tcgcgctgtt gtggttgcgg aataaccatc
    17701 ttcccttcct gcaacagggt gagaatggtt tttaattgct ccggtgagag ctgattcagc
    17761 ggcgtctgac caaagttatg acggatataa tccgtgaccg cctgacgatt attccccaga
    17821 cgtaaatact cccctaattg cgctaaaagc tggcgggcag aatggctttt ttgcgcggcc
    17881 agcagacgtt gcgccagatt atgctcagcg gcagggaagt gacgcgaaag cagcggtgaa
    17941 tctcccgaca gaccaatatc gtgcctgata cccgcccaca gttctgctct ttgctgttgc
    18001 gtcagtgagg tcactttcgt cattaagctt tccagcgaag tacgttgctg actggataaa
    18061 ggctgattgc ccgcgccaga cggcaggtta tctccctgac ctggtggttg cccaggagga
    18121 gggccggaaa taggttgtat cattacgtat ccttatacct gaaatcttcg caagtatgcc
    18181 tggccgcgag attatggcac acttgtccgg ttaactctcg tctcatacag gtaacacaaa
    18241 cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc gacttattta gccgtttggg
    18301 tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct caactggcag acgcggatgc
    18361 gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg ctggcaggaa aacccattaa
    18421 atttgttggc actgccactg cggggaccga ccatgtcgat gaagcatggt tgaagcaggc
    18481 gggaattggt ttttccgctg cacctggctg taatgcgatt gcggtggtgg aatatgtttt
    18541 ctcctcacta ctgatgcttg ccgaacgcga tggattttca ctgcacgacc gtacggtggg
    18601 gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg cgactggaag cgttagggat
    18661 taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg gatgagggtg atttccgctc
    18721 gctggatgaa ttagttcagc atgcggatat tctgactttc catacgccac tcttcaaaga
    18781 tgggccgtac aaaacgctgc atctggcgga tgaaaaactg atccgtagcc tgaagccggg
    18841 agcgattctg attaacgcct gccgtggcgc agtcgtcgat aatactgcgt tgctgacctg
    18901 cctgaatgaa ggccagaagt taagcgtagt gctggatgtc tgggaaggcg aaccggaact
    18961 taacgtggag ttgctgacaa aagtggatat cggcacgccg catatcgcag gttataccct
    19021 ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc tatagcaagt ttattgggca
    19081 tgaacagcac gttgcgctgg atacattact gcccgcgcca gagtttggtc gcattacgct
    19141 gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg catttggtgt atgatgtgcg
    19201 ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg ggtgagttcg ataaactgcg
    19261 caaaaactat cttgagcgcc gtgaatggtc atctctgtat gtaatttgtg atgacgccag
    19321 tgcggcatca ttgctgtgta aactgggttt taacgccgtt catcatccgg cacgttaatc
    19381 tcttcttcat gctctctgct gtaacattgg cagggagctt tgctatttct ggagtaaacc
    19441 accatgtctg aaggctggaa cattgccgtc ctgggcgcaa ctggcgctgt gggcgaagcc
    19501 ctgcttgaaa cgctggctga acgtcagttc ccggttgggg aaatttatgc actggcacgt
    19561 aacgaaagcg ca
//
LOCUS       DACAPB010000076        19397 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.076, whole genome shotgun sequence.
ACCESSION   DACAPB010000076 DACAPB010000000
VERSION     DACAPB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.076"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(87..821)
                     /gene="cysH"
                     /locus_tag="IT904_002429"
     CDS             complement(87..821)
                     /gene="cysH"
                     /locus_tag="IT904_002429"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP3192199.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(896..2608)
                     /gene="cysI"
                     /locus_tag="IT904_002430"
     CDS             complement(896..2608)
                     /gene="cysI"
                     /locus_tag="IT904_002430"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAP3192200.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPARPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2608..4407)
                     /gene="cysJ"
                     /locus_tag="IT904_002431"
     CDS             complement(2608..4407)
                     /gene="cysJ"
                     /locus_tag="IT904_002431"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAP3192201.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQVASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            4723..5088
                     /gene="queD"
                     /locus_tag="IT904_002432"
     CDS             4723..5088
                     /gene="queD"
                     /locus_tag="IT904_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAP3192202.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIFRGE"
     gene            5166..6437
                     /locus_tag="IT904_002433"
     CDS             5166..6437
                     /locus_tag="IT904_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP3192203.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6428..6688
                     /locus_tag="IT904_002434"
     CDS             6428..6688
                     /locus_tag="IT904_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP3192204.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GNLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            6705..7280
                     /locus_tag="IT904_002435"
     CDS             6705..7280
                     /locus_tag="IT904_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAP3192205.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7428..8321)
                     /locus_tag="IT904_002436"
     CDS             complement(7428..8321)
                     /locus_tag="IT904_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP3192206.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSVRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVTRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVG
                     VVDDWKVVLEALVTNIHADCQ"
     gene            complement(8285..9064)
                     /locus_tag="IT904_002437"
     CDS             complement(8285..9064)
                     /locus_tag="IT904_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAP3192207.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9042..10451)
                     /locus_tag="IT904_002438"
     CDS             complement(9042..10451)
                     /locus_tag="IT904_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192208.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10473..11927)
                     /locus_tag="IT904_002439"
     CDS             complement(10473..11927)
                     /locus_tag="IT904_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidoreductase"
                     /protein_id="HAP3192209.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKVTGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(11997..12782)
                     /locus_tag="IT904_002440"
     CDS             complement(11997..12782)
                     /locus_tag="IT904_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3192210.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13100..14377
                     /locus_tag="IT904_002441"
     CDS             13100..14377
                     /locus_tag="IT904_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192211.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSAFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQIAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALVMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14404..15882
                     /locus_tag="IT904_002442"
     CDS             14404..15882
                     /locus_tag="IT904_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP3192212.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWSVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNICNEINHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDNMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     repeat_region   16521..17342
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16522..16547
                     /rpt_unit_seq="ggtttatccccgctggcgcggggaac"
     gene            complement(17682..18353)
                     /gene="queE"
                     /locus_tag="IT904_002443"
     CDS             complement(17682..18353)
                     /gene="queE"
                     /locus_tag="IT904_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAP3192213.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            18492..18632
                     /locus_tag="IT904_002444"
     CDS             18492..18632
                     /locus_tag="IT904_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192214.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNEYKITDAAVNLFI
                     QI"
     gene            18646..>19397
                     /locus_tag="IT904_002445"
     CDS             18646..>19397
                     /locus_tag="IT904_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAP3192215.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQ"
BASE COUNT         4751 a         4991 c         4754 g         4901 t
ORIGIN      
        1 ttcacgttta aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca
       61 gaggcgttta atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag
      121 ccaaagaaac gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta
      181 tcaccgaccg ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc
      241 aggtactgat aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
      301 cgctgaattg ccagcaccgg taaattggca cgactgccgg attgttcacg gcgcaggcca
      361 gcaaaccagg tttgcgcatt cagttctttc agagcccggt tcatcggttc gactttgttg
      421 atgtcattgt acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct
      481 tcctgccagg ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc
      541 gttaactcgt caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc
      601 accggaatat ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca
      661 aagctggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct
      721 tcagcgtcca gtttttccag ttcggcgtta gtttccgcca gcgccagaat gcgatctacc
      781 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
      841 ttgcctgatg cgacgctcac gcgttcttat caggcctaca agaccggact gatggttaat
      901 cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt
      961 cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg
     1021 acgccaggat ttccggctcg gtgatgtttt ctttatacat ccgagggata cgtgtcccaa
     1081 tgcggttgcc gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt
     1141 ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct
     1201 catcgctgac accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga
     1261 aacgctccgc ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccattgagt
     1321 tttcacgctg cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct
     1381 tcgctttctc gctttccggt acaccggcga tgatcagatt ctggttcgcc gtaatgcgga
     1441 aatcgccttt gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgcgccggat
     1501 aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct
     1561 taacccagcc aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt
     1621 tgatccccgc gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg
     1681 tgtatttggt tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca
     1741 cgacggcttc ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcactcgcgg
     1801 tgcgggcgta ggttttcttg ttgccgtgtt cgatggaaag cccaccgccc accaacaggt
     1861 taaagcccac cagcttgccg ttttcggcga tcgccacgaa gttcatgtcg ttggcgtgca
     1921 gatcgatatc gttctgtggc gggatcacta ccgtggtttt gaatttacgc ggcaggtagg
     1981 tctggccgag gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct
     2041 ccgcatacgc gcgggtacgc ggcagcagat gctcagaaat cttcttcgcc cactcgtacg
     2101 cttccgcgtg tagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt
     2161 cgttagcggt cgccagcgca tcaagaccaa ccgagtgcag catctggtgc accggtttga
     2221 cgttcttttt cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc
     2281 catagatggt gttttcaccg gcaaatttgt ctatcgcctg ccactgttta gtggtaatca
     2341 ccccacccgg cagacggcag cgcaacagca tcgcgtggcg cggctccagc ttctgttcag
     2401 cacgttcggc gcggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca
     2461 ggaagttgtc gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc
     2521 cgcgcaggta gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca
     2581 ctaaaggccc tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac
     2641 gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat
     2701 cacttccaga agtgcctgct caacgtcttt cgccatgcga ttagcgtcac cgcagacata
     2761 aatgtgggca ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc
     2821 ttgtacgtaa actttttctt tttggtcgcg cgaccaggcg agatcgatcc gcgtcagcac
     2881 accctctttg acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg
     2941 gttgccaaag aacagccagt tttttcccgg cgcttcatcg gcagcacgtt gctgcataaa
     3001 ggcgcggaac ggcgcgatgc cggtgcctgg gccaatcata atcaccgggg tttctggatt
     3061 ggctggcagg cggaagttat cgttatgttc gataaatacg cggacttcgc cctcttcttc
     3121 cacgcggtca gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta
     3181 acgcaccaca ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcaat
     3241 ggaatagaga cgcggcgtca gcgggcgcag cagattaatt agcgcttcgg catcaagctg
     3301 tgccggggag aaacgcacca tgtcaacaat cggcgtcgtc gcggcgtaat gctgtaactt
     3361 cgctttatcg cccaccagcg gcagcagtgt ttcactgcgg gtaagcgtgg cgtaattctc
     3421 aacaatgttg gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg
     3481 caacgttttg ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag
     3541 ttctttcacc agtgccggat cgttctgata ccagacgccc agcgcgtcac ccggctggta
     3601 acgcaggccc gagtcaccta agtcaatttc gatatggcga acgtcttttt cagagttacg
     3661 cccggtaatt ttctggttaa cagagaggct agccaccagc ggcgcgtctt tgctgtacgg
     3721 gctggtgtgg atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg
     3781 cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta
     3841 ttcaacatcg gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct
     3901 gtcgaaatct ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac
     3961 ggcaaacgcg gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc
     4021 gacggcttct tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt
     4081 ttcgctggcg acttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt
     4141 tgctgctaat aaatcatcac gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga
     4201 ggcggagata atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc
     4261 agcaggctgc tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtggg
     4321 agttaaatcg gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa
     4381 cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc
     4441 tgcgccctgt cagcgcccat aaaacagaag agaaggtaag gttaacgggg caaacggtgt
     4501 ggattaaaga cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag
     4561 tctttaacaa caaaatagat taaccaacct aatgaaaaac aaatgaattt agccaatcat
     4621 taagataaat cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct
     4681 ttccggtact ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta
     4741 tttaaagatt tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa
     4801 tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat
     4861 ccgcatacgg gctggattat cgatttcgct gaactaaaag cggcgtttaa accaacctac
     4921 gagcgcctcg atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgag
     4981 gttttagcaa aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg
     5041 atggtaaaag aaacctgcac cgcaggttgt atctttcgcg gcgaatgata agagtgtgtc
     5101 ggcggtcaat ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc
     5161 atgacatgga agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt
     5221 gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc
     5281 ccggcagcaa aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc
     5341 cgcaatttca tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt
     5401 taacgccgga tggcgcaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg
     5461 tattacgtgc acggttcgat ccgtggctgg ttgccgaagc cgaagaagaa ggtgtcgaat
     5521 gcatccccgg agcgacggtg gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca
     5581 tttgtggtga cgatattctc cgtgcccgtt atgtggtgct ggcagaaggt gccaacagcg
     5641 tcctggctga gcgtcacggg ttagtgactc gtcctgctgg tgaagcgatg gcgttgggga
     5701 tcaaagaagt gctgtcgctg gaaccatccg ctattgaaga acgttttcat ctggagaata
     5761 acgaaggcgc agcgttgctg ttcagcggca ggatctgtga tgacttaccc ggcggcgcat
     5821 ttctttatac taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta
     5881 cgcaaagccg tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc
     5941 gcccgcttat caaaaacacg gaatcactgg agtatggtgc gcatctggtg ccagaaggtg
     6001 gcttgcacag tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt
     6061 tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg
     6121 cgcaggcggc ggcacaaacg ctgataagcg cctgccagca ccgcgagccg caaaatctgt
     6181 ttccgcttta tcatcacaac gtagagcgca gcctgctgtg ggatgttcta cagcgttatc
     6241 agcatgttcc ggcgcttttg caacgcccgg gatggtaccg tacgtggcct gcgttaatgc
     6301 aagatatttc ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt
     6361 tattctggca tcatttacgt cgtcacggcc tgtggcatct ggcgggcgat gttatcagga
     6421 gtctgcgatg tctgtagccc gtaatctctg gcgcgttgct gatgcgccgc acattgttcc
     6481 ggctgactcc gttgagcgcc agacggcaga acggttgatt aacgcctgtc cggcaggtct
     6541 tttttcgctc acaccggaag gtaacttacg tattgactat cgcagttgcc tggagtgtgg
     6601 cacctgccgt ttgctgtgcg acgaatcaac actacaacag tggcgctatc cgccttccgg
     6661 attcggcatc acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc
     6721 tgctccgcca gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcaa
     6781 tcgattctga atgccaattt atttccgtgt tgtacggcaa tatctgcacc atcagtaata
     6841 tcgttaaaaa gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag
     6901 gcgcgtcaaa taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca
     6961 ttatcagtac caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc
     7021 atcgcctgtt tattgttgat tcaatttcgt ttcacaatat tgataagcaa gttgcgcaat
     7081 cgaatccgga ttgtattgag atcctgccag gctgtatgcc caaagtgctg ggctgggtga
     7141 cagagaaaat ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc
     7201 gtaatgcgat taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt
     7261 tagcgaaaaa attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag
     7321 gccgcacgcc acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc
     7381 tacaagtccc caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct
     7441 gcgtgaatgt ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca
     7501 tctgcctgtg aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta
     7561 ttgcgtacgc ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca
     7621 ggtgccagca gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc
     7681 actcgcgccc gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc
     7741 tggttatctg cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg
     7801 tcgcgggtaa catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg
     7861 acaatgttca gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga
     7921 cgcgccaggg aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt
     7981 ccccagtggg atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg
     8041 cttgcaccat gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc
     8101 ggaaagagaa caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc
     8161 tgttccgcca ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa
     8221 tcctgcgccg ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg
     8281 atattcatgg ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg
     8341 ctaccgtttg cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca
     8401 gacactgcat cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg
     8461 cagccattcg ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg
     8521 caatcaccgc aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga
     8581 gggtgataaa cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca
     8641 gcatttccgc cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg
     8701 taattatcag atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt
     8761 ccggcgcaaa gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag
     8821 ccatgagata gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag
     8881 acatcggcgt accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag
     8941 caccgagtaa acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct
     9001 gccactcttt ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt
     9061 tcattcgcac ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc
     9121 catagccagg tcacaacaaa accaaccaac aacactgtcg ccaggagcaa taaggtgact
     9181 tgcattcccc actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt
     9241 cgactcatgg cagtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc
     9301 gcaggcaaaa tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa
     9361 agcagcagcg ttaatgaact gccggaaggc aaacaggcca tgactaccag cgttgccgcc
     9421 agcagcaaaa aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat
     9481 cccagcagcg cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct
     9541 tccagaccaa tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt
     9601 acgaggcaga caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac
     9661 aaggttttga tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga
     9721 ccaaaatagc gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag
     9781 cgtggtgatt ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc
     9841 gatgccagta gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata
     9901 cttgccagca catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga
     9961 tggcggcgcg gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga
    10021 ccaatgccaa tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt
    10081 aaaaacgaag caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata
    10141 tggtcggaga tccacccaag aaccaagctg ccaaggaaca aaccaaggag cgccgagccg
    10201 ccgatcattc ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg
    10261 taaccaatga cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca
    10321 atgcggcagt gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc
    10381 tttacccctt cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa
    10441 cgccccgtca tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc
    10501 gtattcataa tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg
    10561 ctgccgtgtt ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc
    10621 atcgaaccgc cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac
    10681 ttgtcgattt cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg
    10741 ttggtgccgt tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg
    10801 tgcgggaact cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc
    10861 cagcagccgg atacttcggt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt
    10921 tcggcagcca ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg
    10981 ctgtccacgc gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc
    11041 accttcgcaa tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtcggca
    11101 aaatgggtga agtgttgggt gccatcttca gcgtcataca aacgagcgat tgacggacga
    11161 tacccttcca ccatgatttc acgcaggatg ttgaagccgg ttttcatgtc ttccaggata
    11221 tagccgtaga agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg
    11281 atatagcaca atgcaccttc gttgccgatg atgatgtgac gaatgtccgg gccagccgcg
    11341 cggcgtggca cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca
    11401 acgaccatat cttcgattgc gccgtagagt gtggagaact gcccgatact tcgggttgct
    11461 accaggccgc ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct
    11521 ttttcacgca acgcgttttc cagcacttcc agcggaacac cacattgcgc cgtcgcctgc
    11581 atattctcaa tatcaatatt aatgatttga ttcatggcgg agccgtcgag caccaccgag
    11641 ttttctacaa cagtttccag cccaccttcg gtggcggaag caccggtacg cggcacaccg
    11701 ttaattttgt gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt
    11761 tttacgaccg ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga
    11821 aaacggtcaa tactgttttt ctttaatact gtttcatcgg taattacgcg atctgcacca
    11881 acaatttcct ttagctggtc gacaatcgct gcgcgagata aagacatagt aattccttcc
    11941 tgataataaa aatggaataa tcattaataa gagaaaacag aaaccaccgt tatgggttag
    12001 cgcactaaat aaccgccatc aaccactaat aaatgcccgt tgacataatt cgatgccgga
    12061 cttgcgagga atacggctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga
    12121 atatgatcaa gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct
    12181 gttgcataat agccaggggc gataccattt acctgaatat tatattgacc tagttcatca
    12241 caataagctt tggtgaaccc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat
    12301 tgtccaccta agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg
    12361 atcataattt ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc
    12421 atcggatccc agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca
    12481 ttgttaacca gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc
    12541 ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca
    12601 atcatttcct ttgtttcgcc gttatctttg acgaaactag gaataaagat atttgcgcca
    12661 gctttggcca acgccatggc aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt
    12721 gcggttttac ctttcaggga gaaaaaatcc attgagaacg cattgagaga ttcgattgac
    12781 atagttagct ccaatttccg taaggcaaaa aaaagaaagg taacttcccc cgcgagaggg
    12841 aagttacctt tctcattatc tctggtaact ggagataatt tttagcataa gggcataact
    12901 gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc aaataatttt aaaaagcaga
    12961 agcgtgatta ctctcacaca acacctctgc tttccatgtt atttattatt tcaattacta
    13021 gagacctgag aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta
    13081 ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat
    13141 aattagtttt agcggcggcg ttagtttcga cctggcttat ttacgttata tttatcaaat
    13201 tcccatggcg aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtgcctt
    13261 tggtattgcg gccattattc tttatgcccc cagcggcgtt attgccgata aattttcaca
    13321 ccgcaaaatg attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac
    13381 gtatccaccg ctgtgggtaa tgctctgtat tcagatcgcc tttgcgataa cgacgatttt
    13441 aatgctgtgg tcggtgtcga ttaaagctgc atcgttgctt ggcgatcata gcgagcaagg
    13501 gaaaattatg ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt
    13561 taccatgtgg gtcttttctc gctttgcacc ggatgacagc accagcctga aaacggtcat
    13621 tatcatctat agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga
    13681 taacaacaac ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat
    13741 cctggccgtt ttgcgtatca gcaccacctg gtattgcagc atggtgattt ttggcgtctt
    13801 caccatctac gccattctga gttactccac caactatctg accgaaatgt atggcatgtc
    13861 gctggtggcg gcgagctaca tggggattgt gatcaacaaa atattccgcg cgctgtgcgg
    13921 cccacttggc ggcataatca ccacctacag caaagtgaaa tcccctaccc gcgtgatcca
    13981 aatcctttcc gtactcggcc tgctgacgtt aactgccctg ctcgtcacga actctaaccc
    14041 gcaatcggtc gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc
    14101 acgcgggctg tactgggcct gccctggcga agcgagaaca ccgtcttaca ttatgggcac
    14161 cacggtaggt atttgttcgg tgattggatt cctgccggat gtcttcgttt acccaattat
    14221 cggccactgg caagacaccc tgcccgcagc agaagcctac cgcaatatgt ggctgatggg
    14281 catggcggcg cttgtcatgg tgattgtctt tacctttttg ctgttccaaa aaattcgtac
    14341 tgctgatagc gcccccgcaa tggctagcag caagtaagcc aggaaaatta caggagaaaa
    14401 atcatgtcga agaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg
    14461 atgtacgatc tggaaggtaa cgtggtttgc gaaggcaaag gcttattgca gccgatgcac
    14521 acgccagatg ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt
    14581 gccggtcacg atttgatgag tcagtttgcc ggaaataaag aagatattgt cggtattggt
    14641 ctgggctcca tccgttgctg ccgtgcgtta ttgaaagccg atggcacgcc tgctgcgccg
    14701 ttgattagct ggcaggatgc acgcgttaca cgcccttacg aacatacgaa tcctgacgtg
    14761 gcgtatgtca cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac
    14821 aatatcgcca actattttgg tcagtggtcg gtggattata agagctgggc atggagtgaa
    14881 gatgccgcag tcatggagaa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg
    14941 cctggtaccg tcctcggaca tatcacacca caagccgcgc tggcgacaca tttcccggca
    15001 ggactgccgg ttgtttgtac caccagtgat aaaccggtag aagctctggg ggccggatta
    15061 ctggatgatg aaacggcggt aatttctctt ggcacttaca tcgcattgat gatgaacggc
    15121 aaagcactgc cgaaagatcc agtagcgtac tggccgatta tgtcttctat tccgcaaaca
    15181 ttgctgtatg aaggttacgg tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac
    15241 atgctaggcg agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta
    15301 ctcaacaaaa aagcttcttg tgtgccgcca ggctgtaatg gtctgatgac ggtgctggac
    15361 tggctgacca atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc
    15421 atggattacg catggatata tcgttcgata ctggaaagcg tggcgctgac gctgaagaac
    15481 aattacgaca atatatgcaa tgaaatcaat cactttgcga agcatgtgat cattactggc
    15541 ggcggttcga acagcgatct gtttatgcag atttttgccg acgtgttcaa ccttccggca
    15601 cgacgtaacg ccattaacgg ttgtgcaagc ctgggggcag cgattaatac agcggtaggt
    15661 ctggggctat acccggatta cgcaacggct gtcgataaca tggttcgcgt gaaagatatc
    15721 tttataccga ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg cattttcaaa
    15781 gacctaacca aacatactga tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa
    15841 ttggggaatg tggattcgat ccagagctgg tcgaatgcgt aagtgaggaa ggccgggcgg
    15901 gaaactgccc ggcctgaaca tacctgaatg gttatccccg ctgacgcggg gaacataagt
    15961 tcgacaactt aaaaaatgat aacaagtccg gtttatcccc gctgatgcag ggaacataat
    16021 aaaaacttta actagttcgg tttatccctg tttttacaag gaatattaac tacactcgat
    16081 gtctttagaa taaaagacaa tacgaatcgc actttatcaa ttttcactta aagtatgaac
    16141 tggatatctt tattcataag aactattcat caacagcgta tagaggcggt tatgtaaaac
    16201 cactcattag cctcaaaaca aagacaaact tccagattta ttacttaacc aatgaataac
    16261 aagcttaaaa aatatactgt tattctaaag aaaaaattaa caggcattaa ttcaatatta
    16321 aaaaaataat agattaaaat ttcttaacga tttaagaatc atacaaataa cactttgatt
    16381 aaatttaatt ttttgtatcg ataattgtga acctctctgg catggagaac tattttgaac
    16441 atgaggtgtt acgtggatat gttgcttatt acaagtactg ctaatataaa aacttgagaa
    16501 agagataacg ggttatatgg tggtttatcc ccgctggcgc ggggaactcg acagaacggc
    16561 ctcagtagtc tcgtcaggct ccggtttatc cccgctggcg cggggaacac ctgttttcgc
    16621 aaatctatgg actattgcta ttcggtttat ccccgctggc gcggggaaca cgggcgcacg
    16681 gaatacaaag ccgtgtatct gctcggttta tccccgctgg cgcggggaac actggctctg
    16741 caacagcagc acccatgacc acgtcggttt atccccgctg gcgcggggaa cacgaaatgc
    16801 tggtgagcgt taatgccgca aacacaggtt tatccccgct ggcgcgggga acacattacg
    16861 cctttttgcg attgcccggt ttttgccggt ttatccccgc tggcgcgggg aacacgcgtg
    16921 tcggcaaatc ccgaggaaat agaaccccgg tttatccccg ctggcgcggg gaacacacat
    16981 atgatcaggc gtgcgcggcg ctggcactcg gtttatcccc gctggcgcgg ggaacaccga
    17041 atcacgcccc gctttttact gcgtcagcgc ggtttatccc cgctggcgcg gggaacactc
    17101 catgagccaa aatttcaagg ctgggattcg cggtttatcc ccgctggcgc ggggaacacg
    17161 tacatactca aacgattact gaatcacaaa acggtttatc cccgctggcg cggggaacac
    17221 ctgtgctcct gttttgtgcc gtagttaccg atcggtttat ccccgctggc gcggggaaca
    17281 ctccgacact tagtatcatt atggtgtccg acacggttta tccccgctgg cgcggggaac
    17341 actctaatca taacctatta ttaattaatg attttttaag ccagtcacaa tctaccaact
    17401 ttatagtatc acacaaacaa cacatccatt atgttaaaga gcacttaatc cattgattaa
    17461 aaaggtaaat atttaaaata actctataca aactaaaatc taccaaactt taccgcaata
    17521 attttcactc cagcgaaaaa ttaatgccac agaatttgta gagaatgcta atgattagcc
    17581 ctggatgggt aaaatggagg agttttcaga ggcgacaaac aatataaatg agtagaagtc
    17641 tttacaggtt ccacccattt ataacgctta taaatgttta atcaggcaat atttagatat
    17701 ttatgtgttt gcatcgacaa acgccaatta cgcgcaatgc aggtttcaat gcacaaacgt
    17761 gtggcatcat ccttttggct aatcggctgc agtgcaatga ctcgcggttt atcatcggtc
    17821 agcgttgcca gtagttcatc cagtgcttca atatcgcgta cgcgccccac tggatgcttg
    17881 atttcgttgg ctcgctccag tgcctgtgac aacacttcat agccgccgcg catgttcagc
    17941 tttggcgata cggtaaccca ggtattcggt gtgcagcgta cctcatgagt accactggtt
    18001 tcgatctggc agctaaaacc gttcttttcg agcagatcag tcagtggcag caaatcatga
    18061 atgcaaggct caccacccgt aatcaccaca tgccgcgcgg tgtatccctg gcgaccaatg
    18121 acagccagca aatcttcact gctcgcagcc ccccacttat cactctcttt ggtcttcgcc
    18181 agaatgctga aaagggagac ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg
    18241 caccaggcac agccaaccgg gcatccctgt aaacgaataa aaatggcggg aacgccggta
    18301 aagtaaccct caccttgcag ggtctggaac atctcgttaa tcgggtactg catagcattc
    18361 tctgtgaagt ggataattgt taattattgc agatcctgcc acaacaatca tgtcttatta
    18421 acattctgtt acaggcaggt ttaaagaaaa atgcgaaaca tatcgttaat aattaaaggg
    18481 agtaacgtat tatgtcagaa gaaaataaag aaaatggatt taatcatgtc aaaacattca
    18541 ccaaaattat atttattttt tctgtattag tttttaatga taacgaatat aaaattaccg
    18601 atgccgccgt caatttattt atccagattt aatgagaaat ttataatgcg ctatttcata
    18661 ctaatgttca ctttcgtatg ttcctttgtt gcagcccaac caactattgt cccacaatta
    18721 cagcaacaag ttacggatct tacgagtagc ttaaactcac aagaaaagaa agaactgacg
    18781 cacaagttag aatctatttt caataatacc caagtgcaaa ttgccgtatt aattgttccc
    18841 acaaccaaag acgagacaat tgaacaatat gctacaagag tttttgacaa ttggcgttta
    18901 ggagatgcca aacgtaatga tgggatactg atcgttgttg cctggtcgga tcgcactgtc
    18961 cgcatccagg taggttatgg gctggaagaa aaggtaaccg atgctctggc tggagatatc
    19021 atccgtagca acatgatacc cgcatttaaa caacaaaaat tagctaaggg attagagcta
    19081 gctataaacg ctttgaataa ccaactcact tcacaacacc aatatccgac taatccttca
    19141 gaaagtgaat cagcgtcttc cagtgatcat tattactttg ctattttttg ggtatttgca
    19201 gtgatgttct tccctttctg gttttttcat caaggcagta atttttgtcg cgcatgtaaa
    19261 agtggagtct gtatttcagc aatctatctt ttagatttat tcctgttctc cgataaaatt
    19321 ttttccattg ctgtattttc cttctttttt actttcacca tatttatggt ctttacctgt
    19381 ttatgtgtac ttcagaa
//
LOCUS       DACAPB010000077        19338 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.077, whole genome shotgun sequence.
ACCESSION   DACAPB010000077 DACAPB010000000
VERSION     DACAPB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.077"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..328)
                     /locus_tag="IT904_002446"
     CDS             complement(<1..328)
                     /locus_tag="IT904_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP3192216.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKG"
     gene            complement(346..648)
                     /gene="rplW"
                     /locus_tag="IT904_002447"
     CDS             complement(346..648)
                     /gene="rplW"
                     /locus_tag="IT904_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP3192217.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(645..1250)
                     /gene="rplD"
                     /locus_tag="IT904_002448"
     CDS             complement(645..1250)
                     /gene="rplD"
                     /locus_tag="IT904_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP3192218.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(1261..1890)
                     /gene="rplC"
                     /locus_tag="IT904_002449"
     CDS             complement(1261..1890)
                     /gene="rplC"
                     /locus_tag="IT904_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP3192219.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(1923..2234)
                     /gene="rpsJ"
                     /locus_tag="IT904_002450"
     CDS             complement(1923..2234)
                     /gene="rpsJ"
                     /locus_tag="IT904_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP3192220.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(2472..2891)
                     /gene="gspB"
                     /locus_tag="IT904_002451"
     CDS             complement(2472..2891)
                     /gene="gspB"
                     /locus_tag="IT904_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HAP3192221.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            complement(2893..4362)
                     /gene="gspA"
                     /locus_tag="IT904_002452"
     CDS             complement(2893..4362)
                     /gene="gspA"
                     /locus_tag="IT904_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HAP3192222.1"
                     /translation="MSTRREVILSWLCEKRQTWRPCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKMGLAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            4542..5356
                     /gene="gspC"
                     /locus_tag="IT904_002453"
                     /pseudo
     CDS             4542..5356
                     /gene="gspC"
                     /locus_tag="IT904_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
     gene            5340..7292
                     /gene="gspD"
                     /locus_tag="IT904_002454"
     CDS             5340..7292
                     /gene="gspD"
                     /locus_tag="IT904_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HAP3192223.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLRVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            7302..8783
                     /gene="gspE"
                     /locus_tag="IT904_002455"
     CDS             7302..8783
                     /gene="gspE"
                     /locus_tag="IT904_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HAP3192224.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            8780..9976
                     /gene="gspF"
                     /locus_tag="IT904_002456"
     CDS             8780..9976
                     /gene="gspF"
                     /locus_tag="IT904_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HAP3192225.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            9986..10423
                     /gene="gspG"
                     /locus_tag="IT904_002457"
     CDS             9986..10423
                     /gene="gspG"
                     /locus_tag="IT904_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HAP3192226.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            10431..10940
                     /gene="gspH"
                     /locus_tag="IT904_002458"
     CDS             10431..10940
                     /gene="gspH"
                     /locus_tag="IT904_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HAP3192227.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            10937..11314
                     /gene="gspI"
                     /locus_tag="IT904_002459"
     CDS             10937..11314
                     /gene="gspI"
                     /locus_tag="IT904_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HAP3192228.1"
                     /translation="MKKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            11307..11894
                     /gene="gspJ"
                     /locus_tag="IT904_002460"
     CDS             11307..11894
                     /gene="gspJ"
                     /locus_tag="IT904_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HAP3192229.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDSRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            11887..12870
                     /gene="gspK"
                     /locus_tag="IT904_002461"
     CDS             11887..12870
                     /gene="gspK"
                     /locus_tag="IT904_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HAP3192230.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            12885..14048
                     /gene="gspL"
                     /locus_tag="IT904_002462"
     CDS             12885..14048
                     /gene="gspL"
                     /locus_tag="IT904_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HAP3192231.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGN"
     gene            14045..14506
                     /locus_tag="IT904_002463"
     CDS             14045..14506
                     /locus_tag="IT904_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HAP3192232.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            14506..15183
                     /locus_tag="IT904_002464"
     CDS             14506..15183
                     /locus_tag="IT904_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP3192233.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(15212..15688)
                     /gene="bfr"
                     /locus_tag="IT904_002465"
     CDS             complement(15212..15688)
                     /gene="bfr"
                     /locus_tag="IT904_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAP3192234.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(15760..15954)
                     /gene="bfd"
                     /locus_tag="IT904_002466"
     CDS             complement(15760..15954)
                     /gene="bfd"
                     /locus_tag="IT904_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAP3192235.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(16123..18816)
                     /gene="chiA"
                     /locus_tag="IT904_002467"
                     /pseudo
     CDS             complement(16123..18816)
                     /gene="chiA"
                     /locus_tag="IT904_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
     gene            complement(19108..>19338)
                     /locus_tag="IT904_002468"
     CDS             complement(19108..>19338)
                     /locus_tag="IT904_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP3192236.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
BASE COUNT         4956 a         4996 c         4514 g         4872 t
ORIGIN      
        1 ggcctttagg ggccaggatg taacggcgtt caccgtcttt gtacagaacc agcgcgatgt
       61 tcgcggaacg gttcggatcg tactcaagac gttcaacaac tgccgggata ccgtctttgt
      121 tgcgtttgaa gtcaacaata cggtaagcct gcttgtggcc accaccgata tgacgagtgg
      181 tgatacggcc attgttgtta cgaccaccgg atttgctgtt tttttccagc aacggagcaa
      241 aaggtttgcc cttgtgcagc tcagggttaa ccactttaac tacgtggcga cgacccggag
      301 atgtcggttt acatttaaca actgccattg tattactcct ccgacttact cagcgccgcc
      361 aacgaagtcc agattctggc cttctttcag ggtgacgtaa gcttttttcc agtcgctacg
      421 acgaccgata cgctgtccgt gacgtttaac tttcccttta actaccaggg tgttaacgac
      481 ttcgacttcg acttcaaaca gtttctgcac agcagctttg atttctgctt tggtcgcgtc
      541 tttagcaact ttgagtacga tggtgttgga tttttccatc gcagtagacg ctttttcaga
      601 aacgtgcggt gcacgcagca ccttcagcag acgttcttca cgaatcatgc cagcatctcc
      661 tcaacttgct taacagcatc agcagtcatt acgactttgt cgaaggcgat caggctaacc
      721 gggtcgatac cagttgcatc gcgtacgtca accttgtgca ggttgcgcgc agccaggaac
      781 aggttttcgt ccagctcacc ggtgatgatc agcacatctt ccagagccat gtctttcagt
      841 ttctgtgcca gcagcttagt tttcggcgct tctacagaga acttctcgac aacgatcaga
      901 cgatcctgac gtaccagttc ggacaggatg cttttcagcg cgccgcggta catcttcttg
      961 ttaacttttt gactgtggtc ctgcggacga gcagcaaagg tcacgccacc agaacgccag
     1021 atcgggctct tgatagaacc agaacgcgca cggccggtgc ctttctggcg ccacggtttt
     1081 ttaccggaac cagttacttc agcacgagtc ttctgagcac gagtaccctg acgagcacca
     1141 gctgcataag caacaacaac ctggtgaacc agcgcttcgt tgaaatcacg accgaaggta
     1201 gtttcggaaa cagtcagcgc gctctgcgcg tctttcaata ctaattccat tgctatctcc
     1261 ttacgccttc acagctggtt taacgatcag gtcgctaccg gttgcacccg ggacagcacc
     1321 tttaaccagc agcaggttgc gctcagcgtc aacgcgtact acgtcaaggc tctgaacggt
     1381 tacacgttcg ttacccatct gacctgccat tttcttgcct ttgaacactt tgcccggagt
     1441 ctggttctga ccgatagaac ccggaacgcg gtgagacaag gagttaccgt gagtagcgtc
     1501 ctgggtacgg aagttccagc gcttaacggt acctgcgaaa cctttacctt tagaggtgcc
     1561 agttacgtca acttttttaa cgtcagcaaa cagttcaacg ctaatgctct gacctacagt
     1621 gaactcttcg ccttcagcca ggcggaattc ccacagacca cggccagctt ctacgccagc
     1681 tttagcgaag tggccagctt caggcttggt cacacggtta gcttttttag caccggtggt
     1741 cacctgaata gcacggtagc catcgttagc caggtcttta acctgagtaa cgcggtttgc
     1801 ttcaacttcg attacggtta ctgggataga aacgccgtct tctgtgaaga tacgggtcat
     1861 acccactttt ttaccgacta aaccaatcat tgtttcaacc tctcaatcgc tcaatgacct
     1921 gattaaccca ggctgatctg cacgtctaca ccggcagcca gatccagacg catcagagca
     1981 tcaacggttt tctcggttgg ctcaacgatg tcaaccagac gcaagtgagt acggatttcg
     2041 tactgatcgc gcgcgtcttt gttgacgtgc ggggagatca gaacagtgaa gcgctctttg
     2101 cgtgtcggca gcgggatcgg accacggacc tgcgcaccag tgcgcttggc agtctcgacg
     2161 atttccgcgg ttgcttgatc gatcagacga tgatcaaacg ctttcaggcg gatacggatt
     2221 ctttggttct gcatgagacc agagctccaa ttattttata aacgaaaatg attactcctc
     2281 agacccatta cgattgatgg gagagtgtaa ccgttcttac gtagctcccc gattgggagc
     2341 attgttaggt agccaaattc ggctaactga ggttcagatt gaacctgctg tcaactacga
     2401 caagcccgcg cattatacat acttaaccac cgaacgcaag cgtattgcta gtaaagtaaa
     2461 cagccttcgg gttatctctc aagttcgttc agtgcatttt tgacacgctc tcttaaaccg
     2521 gctttctcgt cgctttcttc cacagtattt tcttcgtctt cagaagaaga gggggcattc
     2581 tcaaccgcga cgcctgggtc atcttcctcc tcgacggatt cgacgacggg aagtggctgt
     2641 tttttgaaga aatagtgttg tgttgattgt ttaaaagcgg gcgtttcagc ctctacagtg
     2701 acttcggctt ttactatcca aagcacccat agctgcctgg catacccacc aaccagcacc
     2761 attcctgcaa accacaggca aatcagtaat aaacaaatta catatattat ggtgctgtgt
     2821 ttttgtcggg agaagatacc agggtgacct gtttctttct gctcacgcgc ggcgatatag
     2881 aattcaaaca tgtcatttcc ccttcactgt tgtttccggg gaaatatttg cttcatcccg
     2941 atataagtag gcactctccc ctgccacctg ccagagatgt actaacgttg aaaagccaac
     3001 aacaccgtca gttttcaagt ggtggctttt ttgaaattgc tttatttttt ctaccaacaa
     3061 gggacgccac tccgccgaag gttctgtcga aatatgcaat gcgcggttta acatcgtttc
     3121 cagccagaga atctcttcct cgctgctatc acgcgtaatg gtactctccc cttccggcga
     3181 cattttccac aacagaagat aatcacccgt ccataccgac tcaaaccatt tatgtgtcag
     3241 cgtccacgtt tgctgaccta cgaggacatc aacgctagcc tcaccgacgc gaacgaccac
     3301 aacaggcaat tttttgtccc cgactttaag cgatgcgatc cagggcaaat tctgatctac
     3361 caatgtttgt aaagaagcat tcccactttt acatgccagc cccgctctta ctgcctgatc
     3421 gcaccaggcc gaatcagctg ggacctcgta cccccatacg ccgtataaca cgcttagcgc
     3481 ctcactatcg cgcatcacca catgagcaat atccttagtc atatcttgtt taactactgg
     3541 cgttacgggt atcagccacg caggaacggg taacgtagct gtaaaagagg acagcagtag
     3601 ccaaccacag gtaagagcga taatggttcc actcatcacc acggcaagct gcattttccg
     3661 ggggcggtta tcacccaata tgcggtaaat cattcgccat tgttttgctg tgaaaacgct
     3721 ggtctgctcg gcttgctgta ataagcgaat tgtttcgccg gcaagcccca tctttctgcg
     3781 atcgccccga cataataaag ctatccgttt cagaactctg gcggttaaaa ggagatcggg
     3841 ttggtcaata ttctgacctt caaggatgct tgccttaagt tcgtgctccg tcagacgtcc
     3901 tatcgagaca agttcaatgc cctgtttttt aaaatcacgt tcgacttttc gatcgggtct
     3961 gccgatcagc aaaaattgac cgtcaggaat aagagatatt gcgcgttgga gatcatcaag
     4021 tatgcggcgg tgattcaggt gagcattttc aataataata agagggcagc gactatcagt
     4081 tcgatgaagc gcatgcagta attgccccgc catctcatct cgcgtccagg cgctgtcacg
     4141 gcagccctgt tcagccgcgt tatcgtcggt aacgatccat gcggccttac cttgccagga
     4201 aacaacgagg cttaaagtaa tcacacggcg atgtttatct ttttgcagtt gctgcgccag
     4261 ccaggttttt ccacttccag cctcacccaa caaatagcat ggacgccagg tttgacgttt
     4321 ctcacacaac caggaaagaa taacttctct tctcgtagac atagaacttc ctgttttaat
     4381 tattgattat ccttaatcat aaaaataatg tatgtattta ttaattaaat acatattact
     4441 aatataaata aattttgctt gattcatgca agcggcatta atactattta tactaacgtc
     4501 aatatacaac ccacccaatc tattttatta gaacatacat cgtgcccaca ctacgtttcc
     4561 ccttccatct ggcgaatcat aataaagatg ctgccatcaa tatattgatt atatttataa
     4621 gcatagggtc gattattttt aatgtgaatt atttccatac aactatagtt aaaaatggac
     4681 agatcattaa tcaaccaaca aagcatttca gtctgatttc agccttgccg ctttatggcg
     4741 taacgaaaat catgctgggg taaaagatgc aaatcccgtg gcagttaatc aggaaacacc
     4801 taagctctct atagcgctta atggcattgt actcacatcg aatgatgaga cttcttttgt
     4861 cttaattaat gaggggagcg aacaaaagcg atactccctc aacgaagccc ttgagtcagc
     4921 gccgggaacg tttattagaa aaataaataa aaccagcgtt gtgtttgaaa ctcacgggca
     4981 ttacgaaaaa gtaactctcc atccagggct acctgacatc atcaagcagc ctgattcaga
     5041 aagtcaaaat gtactcgctg attatattat cgccacgcct atacgtgatg gagaacagat
     5101 atacgggctt cgattgaacc ctcgtaaggg cctcaatgca tttaccacca gcttgctaca
     5161 accaggggat atcgccctca ggatcaataa tctttcgctg acccaccctg atgaggtttc
     5221 acaggcatta agtttactgc taactcaaca aagtgctcag tttacaattc gtcgcaacgg
     5281 cgtaccccgc ttgataaatg tttccgtcgg ggaacttaca ggaatgaatg gactgcgtca
     5341 tgaaaggact caataaaatc acctgctgct tgctggcagc actactcatg ccttgtgcag
     5401 gacacgctga gaacgaacaa tacggcgcga acttcaataa cgccgatatc cgccagttcg
     5461 tggaaatagt gggtcagcat cttggcaaaa cgatcctgat cgacccttcg gtacagggaa
     5521 ccatttccgt acgcagtaat gatacgttta gccaacagga gtactaccag ttctttttaa
     5581 gtattcttga tctttacggt tattccgtga tcacgctgga caatggtttt ctgagagtgg
     5641 ttcgctcagc taatgtaaaa acatcgccag ggatgattgc tgacagttct cgtccaggcg
     5701 taggtgatga gttggtcacc cgaatcgtac cgcttgagaa cgttcctgct cgtgacctgg
     5761 cccccctgct ccgccagatg atggatgcgg gtagcgtcgg taatgttgtg cattatgaac
     5821 cctccaacgt tcttattctg accggtcgtg cctccaccat taataaactg attgaagtca
     5881 taaagcgcgt tgatgtcatc ggcacagaga agcagcaaat tattcatctg gaatatgcgt
     5941 cagcggaaga tctcgccgag attcttaatc aattaatcag cgaaagccac ggtaaaagcc
     6001 agatgccagc cctcctctcc gcgaagattg tggcggataa gcgaaccaac tctcttatca
     6061 tcagtggacc ggaaaaagca cgccagcgca tcacttcatt actgaaaagc cttgatgtcg
     6121 aagagagcga ggaaggaaat acccgggttt attacctgaa atatgctaaa gccacgaatc
     6181 tggtggaagt gctaaccggt gtttccgaaa agctgaaaga tgaaaaaggg aatgcgcgta
     6241 agccctcctc ttctggcgcg atggataacg tcgccattac cgccgatgaa cagactaact
     6301 ctctggtcat taccgctgac cagtccgtcc aggaaaaact cgccacggta attgcgcgtc
     6361 tggacattcg ccgtgcacag gtgctggttg aggcaatcat cgttgaagtt caggatggaa
     6421 atggactaaa cctcggcgtg caatgggcga ataaaaacgt tggcgcacag caatttacca
     6481 ataccggatt accgattttt aacgctgcgc aaggtgtggc tgattataaa aagaatggtg
     6541 ggatcaccag cgcgaatcct gcctgggata tgtttagcgc ctacaatggc atggccgcag
     6601 gcttcttcaa tggcgactgg ggagtactgc ttaccgcgct ggccagtaac aataaaaatg
     6661 acatcctcgc caccccaagc atcgtaacgc tggataataa actcgcgtcc ttcaacgtgg
     6721 ggcaggatgt gccggtgcta tccgggtcac agaccacttc aggggataac gtctttaata
     6781 ccgtcgaacg caaaacggtg gggacaaaac tcaaagttac tccgcaggtc aatgaaggcg
     6841 acgcggtgtt gctcgaaata gagcaggaag tctccagcgt tgactcttcc tctaactcga
     6901 cgctcggccc gacgtttaat acccgtacta ttcaaaacgc cgtgctggtc aaaaccggtg
     6961 aaacggtggt cctgggcgga ttgctggatg atttttctaa agagcaagtg tcaaaggttc
     7021 ctctgcttgg cgatattcct ttagtggggc aactcttccg ctatacctcc accgagcgcg
     7081 ctaaacgcaa cctgatggta tttatccgtc cgacgattat ccgtgacgat gatgtttatc
     7141 gctcactgtc aaaagagaaa tacacccgtt accgtcagga gcaacaacag cggatcgacg
     7201 ggaaatcaaa agcgctggtt ggctcggaag atttgccggt gctggatgaa aacacgttca
     7261 acagtcacgc ccctgcgcca tcgtcacggt gaggcattca tatgagaatt cactcaccgt
     7321 accccgccag ttgggcgctg gcacaacgaa ttggttatct ctattcagag ggcgagatta
     7381 tttatctcgc cgatacgcca ttcgagcggt tactcgatat tcaacgtcag gttggtcagt
     7441 gccagaccat gaccagtttg tcacaggctg atttcgaagc ccggctggaa gcggtgttcc
     7501 atcagaatac cggtgagtcg caacagattg cgcaggatat cgatcaatcc gtcgatcttc
     7561 tctcgctttc ggaagagatg cccgcaaatg aagatctcct gaatgaagat tcagcggcac
     7621 cggttatccg cttgatcaat gcgattttga gtgaggccat caaagaaacc gcctctgata
     7681 tccacattga aacctatgaa aaaacaatgt cgatccgttt tcgcatcgac ggcgttttgc
     7741 ggacaatttt acagccaaac aaaaaactgg cggcactgct tatctcccga attaaggtca
     7801 tggctcgtct tgatatcgcc gaaaaacgta ttccacagga tggaagaatt agtttgcgta
     7861 tcgggcgacg taacatagat gtccgcgtat ccacactgcc gtccatctat ggtgaacgcg
     7921 ccgtactccg cctgctggat aaaaacagcc tccagctttc attgaacaac ctggggatga
     7981 cggcagcgga taagcaggat ttagaaaatc tcattcagct tccgcacggt attatcctgg
     8041 tgacagggcc gacaggctcc ggtaaaagca ccacgctcta cgccatcctt tcggcgctga
     8101 atactcccgg ccgcaatatt ctgacggtag aagatcccgt ggaatatgag ctggaaggca
     8161 ttgggcaaac gcaggtgaat acccgtgtgg atatgtcttt cgctcgcggc ctgcgcgcca
     8221 tacttcgcca ggacccggat gtcgtcatgg tgggggaaat tcgtgataca gaaaccgcgc
     8281 agattgcggt tcaggcctcg ctcaccggcc atctggtact ctcaacactc cacactaaca
     8341 gtgcatcagg cgcagtgacc cggctccgcg acatgggcgt cgaatcattc ctgctttcgt
     8401 cttccctggc agggattatc gcgcaacgtc tggttcgtcg cctgtgtccg caatgccgac
     8461 aattcacgcc cgtatcaccc caacaagcgc agatgtttaa atatcatcag ctcgcggtga
     8521 caacaattgg cactcccgta ggctgccctc attgccatca atccggctat caggggcgca
     8581 tggcgatcca cgaaatgatg gtggtgacgc cggaattacg ggccgctatt catgaaaatg
     8641 tggatgaaca agcactggag cgactagtcc ggcaacaaca caaggcctta atcaaaaatg
     8701 gcctgcaaaa agtgataagc ggtgacacct cctgggatga ggttatgcgc gtcgccagtg
     8761 ccacgctgga gagcgaagca tgaattatcg ctatcgcgcc atgacccagg atggtcaaaa
     8821 attgcaaggg atcattgatg ctaacgatga acgtcaggca cgactgcggc tgcgtgaaga
     8881 agggcttttc ctgctggata ttcgccccca aaaaagttcg ggagtaaaaa cacgtcgccc
     8941 gaggatcagc catagtgaac tgacgctttt cacccggcag ttggcaacct taagcgcagc
     9001 ggcattaccc ctggaagaga gccttgccgt aatcggtcaa caaagcagta ataaacgact
     9061 gggtgacgtg ttaaatcagg tacgcagcgc catccttgaa gggcatcccc tttccgatgc
     9121 attacagcat tttcccacgc ttttcgattc gctctatcgt accctggtaa aagcgggcga
     9181 aaagagcggg ctgctggccc cggtgttgga aaagctggct gattacaatg aaaaccggca
     9241 gaaaatccgc agcaagctca ttcagtcact gatctacccc tgtatgctca ctacggtggc
     9301 gattggggtc gtgattattc tcctcactgc tgtcgtgccc aaaattaccg aacagttcgt
     9361 gcatatgaag cagcaactgc cgctgagtac acgcattctt ttaggtctga gcgacacgtt
     9421 gcaacgtacc ggcccgacat tattagcgac agtgtttatt gtcgctgtag gtttctggct
     9481 ctggttaaaa cgcggcaata accgccaccg ttttcatgcc atgttgctgc gcgttgcgct
     9541 catcggcccg ctgatttgcg ccattaacag cgcacgctat ctccgcactt taagtatttt
     9601 gcaatccagc ggcgtccctc tgctggatgg gatgaatttg tccaccgaaa gcctcaacaa
     9661 cctcgaaatt cgccagcgtc tggcaaatgc ggcagagaac gttcgccagg gtaacagcat
     9721 tcatctttcg ctggaacaaa ccgcaatttt cccgccgatg atgctctaca tggtggcctc
     9781 tggcgaaaaa agcgggcagc tcggcacatt aatggtcaga gccgcagata accaggagac
     9841 actccaacaa aatcggatcg ccttaacgct ctccatcttc gagccagcac tcattattac
     9901 gatggcactg atcgtcctgt ttattgtcgt gtcggtactc caacctcttc ttcaacttaa
     9961 ctcaatgatt aattaaggaa agcatatgcg cgcaacggat aagcaacgcg gttttacatt
    10021 actggaaatt atggtggtga tcgtcattat cggcgtactt gccagcctgg tggttcctaa
    10081 cctaatgggc aataaagaaa aggcggataa gcaaaaagcc gtcagcgata ttgtggcgct
    10141 ggaaaacgcc cttgatatgt acaaactcga caatcatcac tatccaacca caaatcaggg
    10201 gcttgaatcc ttagtagaag cgccgacact gccaccgctg gccgcaaact ataacaagga
    10261 aggttatatc aagcgtctgc ctgccgatcc ctggggcaat gattatgtcc tcgttaatcc
    10321 tggtgaacat ggtgcatacg atctgctttc agcagggccc gatggtgaaa tgggaaccga
    10381 ggacgacatc accaactggg gtttgagcaa gaagaaaaag taagtcagcg atgaatcagc
    10441 aacgcgggtt taccctgctg gagatgatgc tggtgctggc gctggtcgct atcacggcaa
    10501 gcgtggtgct cttcacatat ggccgggagg atgtcgccaa cactcgtgcg cgcgaaacag
    10561 cggcgcggtt taccgctgca cttgaattag ccatcgaccg cgccacgctc agtggtcagc
    10621 ctgtcggtat ccatttctct gattcagcat ggcgcattat ggtaccgggc aaaacaccat
    10681 cagcctggcg ctgggttcca ttacaagaag atgccgcaga cgagagccaa aatgactggg
    10741 atgaagaact ctcaattcat cttcagccat ttaaacctga tgacagcaac cagccacagg
    10801 ttgtgattct ggccgacgga caaatcacgc ccttctcttt gttgatggct aacgccggga
    10861 ccggtgagcc gctcctgacg ttagtctgtt caggctcatg gcccctggat caaacgctcg
    10921 ccagagatac ccgcccatga aaaaacaatc agggatgaca ctgcttgaag tcttactggc
    10981 gatgagtatt ttcaccgctg ttgctctgac tttaatgagc agtatgcaag ggcaacgaaa
    11041 tgccatcgag cgaatgcgta acgagacgct ggcattgtgg atagcggata accagcttca
    11101 gtcacaagat tcattcggcg aggaaaacac gtccagttct ggcaaagagt taataaatgg
    11161 cgaagagtgg aactggcgca gcgatatcca ctcaagcaaa gacggcacgc ttctggaacg
    11221 caccataacg gtgacattac ccagcggtca gacaacctcg cttacgcgtt atcaaagcat
    11281 cgataataaa tccgggcagg cacaagatga ttaatcgcca gcagggtttc acattgctgg
    11341 aggtcatggc ggcgctggcg attttctcaa tgcttagcgt gctggcgttt atgatttttt
    11401 cgcaagcttc cgagttgcat caacgctcac agaaagaaat ccagcagttc aatcagctac
    11461 agcgcaccat caccattctt gataacgatc tcctgcaact ggtcgcccgg aggaatcgga
    11521 gtacggacaa aatcatggtg ttgggtgaag aagctatttt taccacgcaa agtcgcgatc
    11581 cgctggctcc tctcagtgaa gcacaaacct tactcactgt tcactggtat ctgcgaaatc
    11641 atacgctcta ccgtgctgtt cgtacttctg tggatagcag gaaggatcaa cccgcccagg
    11701 cgatgctgga acatgtcgag agctttcttc tggaaagtaa cagcggggaa agccaggaac
    11761 ttcctctctc agtcacgtta catctgcaga ctcagcaata tggggggtta cagcgccgtt
    11821 ttgcattacc tgagcagtta gcgagagaag agtcgccagc tcagacgcag gccgggaata
    11881 ataaccatga ataatgaaca acgcggcgtc gcgctgctca ttgtactcat gttactggcg
    11941 ctcatggctg ctctggcagc agacatgacg ctcagttttc acagccagct tcagcgaacc
    12001 cgccaggtaa accatcatct gcaacggcaa tatgatattg aactggcgga gaaacttgcg
    12061 cttgccagcc tcacccagga cgttaaagat aacgacagac agaccacgtt gcagcagtac
    12121 tgggcacagc cacaacagtt gcagctggaa gatggcaata cagtgaagtg gcagttacgc
    12181 gatgcccagc actgttttaa tctcaatgcg ttagcaaaaa tctctgacga cccgctggct
    12241 tcccctgatt tccccgccca ggtattcagt gcgctgctga ttaacgccgg tatcgatcgc
    12301 ggaaacaccg atgagatcgt gcaatcaatc gctgactata ttgatgtcga tgactcacca
    12361 cgctttcacg gcgcagaaga tagcttttac caaagccaga cgccgccccg gcatagtgcc
    12421 aatcagatgc tttttctgac tggcgaatta cgtcagataa aaggcataac cgaaaacatc
    12481 tatcagcggc ttatcccgta tgtctgcgtt ctcccgacca cggaacttag catcaatctt
    12541 aatatgctaa cggagaacga tattccgctc ttcagagcgt tattcctcaa taacatcacg
    12601 gatgctgacg ctcgggtctt attgcaaaaa aggccgaggg aaggctggct aaccacggat
    12661 gcatttcttt actgggcgca gcaggatttc tccggtgtga agccactggt tgctcaggtg
    12721 aaaaggcatc tctttcccta tagccgctat tttacgctga gtacggaaag tatcagcgac
    12781 gaacaatccc agggatggca aagccatatt tttttcaacc gtaaacagca gagcgcccaa
    12841 atctatcggc gtacgctgca actctactaa tcataaggat cgtgatgcct gaatcgttga
    12901 tggtcatacg ttcttcctcg acgctgcgta agcattggga atggatgacg ttctccgcag
    12961 atagcgtatc ttcggtacat acgctcacgg acgatcttcc gctggagtcg ctggccgatc
    13021 agcccggtgc cggaaatgtt catcttttga tcccaccaga gggcctttta tatcgttccc
    13081 tcacgctgcc caacgccaaa tacaaactca ccgcacaaac attacaatgg ctggcagagg
    13141 agacgttacc cgacaacacc caggactggc actggacggt tgtcgataaa caaaacgaaa
    13201 gtgtcgaggt gataggcatt cagtcggaga aactcagtcg ctatcttgag cgtttacata
    13261 ctgccggact caacgtgacg cgggttttgc cagatggctg ctatcttccc tgggaagtag
    13321 atagctggac tctggttaac cagcaaacaa gctggctcat tcgcagtgcc gcacatgcct
    13381 tcaacgaact ggatgaacac tggttacagc atcttgctgc ccagtttccg cctgagaata
    13441 tgctttgtta tggtgtagta cctcacggcg ttgcagcggc caatccactt atccagcatc
    13501 ctgaaatacc atcactgagt ttgtattctg ccgatattgc attccagcgc tatgacatgc
    13561 tgcatggtat ttttcgtaaa cagaaaactg taagcaaatc gggaaaatgg ctcgcccgcc
    13621 tggcagtaag ctgtctcgtt ctcgccattc tttcttttgt tggcagccgg agcatcgcgc
    13681 tctggcatac gcttaagatt gaagatcaac ttcagcaaca acagcaagaa acctggcaac
    13741 gttatttccc gcagatcaaa cgcacccaca actttcattt ttactttaag cagcaactcg
    13801 ctcagcaata tcctgaagct gtaccgttgc tctatcattt acaaacactt ctgctggaac
    13861 accctgaact gcagttaatg gaggcgaact atagccagaa gcaaaagtcg ctgaccttaa
    13921 aaatgagtgc taagagtgaa gccaatatcg atcgtttctg tgagttaaca cagtcctggc
    13981 ttccgatgga aaaaacagaa aaagatccgg tcagtggtgt atggacagta aggaactcag
    14041 ggaattgatt aaatcatggt gggcagaaaa atccacatcg gaaaaacaga tcgttgcggc
    14101 actggcggtt ctctcgctcg gcgttttttg ttggcttggg gtgataaaac cgatagatac
    14161 ctacattgca gagcatcagt cccacgcgca aaaaattaag aaggacatca aatggatgca
    14221 ggatcaagcg agcacccatg gtttactcgg tcatcctgcg cttacgcagc ccatcaaaaa
    14281 tatccttctc gaggaagcaa aacgtgagaa tttggctatc acgctggaaa acggcccgga
    14341 caatactctg acaattcatc ctgtcacagc tcccctcgaa aacgtctccc gctggctcac
    14401 tacggcacag gtaacatacg gtattgttat tgaagacctt cagtttactc ttgccggaaa
    14461 cgaagagatc accctcaggc atttgtcctt cagggagcaa caataatgac aatgctactg
    14521 ccgctcttca ttctggttgg ttttattgca ggttattttg ttaatgccat cgcctatcac
    14581 ctctcgccgt tggaggataa aacggcgtta acgtttcacc aggtattggt tcatttcagg
    14641 caaaaaaaat atgcctggca tgatacagtg cccctgatac tttgtgttgc tgctgctatc
    14701 gcctgcgccc tggcaccttt cacgcccatc gtgactggcg cactctttct ctatttctgt
    14761 ttcgtactca cgctcagtgt tattgatttt cgcactcagc tcctgcccga caaactcacc
    14821 ttaccgctgc tctggcttgg cttggtattt aatgcgcagt atggattaat tgatttacat
    14881 gatgcggttt acggcgcggt agcgggctat ggggtgctgt ggtgtgttta ctggggcgtc
    14941 tggttagttt gtcacaaaga gggattgggc tacggtgatt tcaagctact ggccgctgca
    15001 ggcgcatggt gtggctggca aacgttgcca atgatactgc tgattgcctc gctgggtggc
    15061 attggttacg ccatcgtttc acaacttctg caacgccgga ctataaccac tatcgcattc
    15121 ggcccgtggc ttgcgctcgg cagcatgata aacctggggt atctggcctg gatctcttat
    15181 taaccgggag ggttctccct cccgacacgg ctcaaccttc ttcgcggatc tgtgcttgca
    15241 gataattttg caggcccatc ttctgaatca gatcaagttc cgtttccagc cagtcgatat
    15301 ggccttcttc atcacgcaaa atttctatca tcatatcgcg gctgacgtaa tcatgaacgc
    15361 tatcggcata accaattgcc tcacgcaaat tcttcgcgcc atccagctca agtgccagat
    15421 cagaacgcag catttcctca acatcttcac caatgttcag tttgcccaag tcctgtaagt
    15481 ttggaagacc ttccagaaaa agaatgcgct caatataacg atcggcgtgt ttcatctcat
    15541 caatggattc atgatactcc acatcattga gacgtttgag accccagttt ttaaacattc
    15601 gggcatggag aaagtactga ttgattgcga caagctcatt tcccaacagt ttgttgagat
    15661 aatttataac tttagtatca cctttcattt tttgactcct tcgcttccac tctttgaagc
    15721 gtagaacggc ttacgagtaa gtcaaaaaac acactccgtt tatgcggact ccttaaactc
    15781 cggcagctgc attaattcat cctccatcac ttcacgcgcg gcccggacac atttaccgca
    15841 ctgattgccg accggaataa atttttttaa ttgctggaac gagtgagggg aaaactggcg
    15901 cacagcctga cgaatttttt tatcgctgat accattacaa agacaaacgt acatagagac
    15961 tcccgtatac tttcttcaaa aataaatgaa aatagttctt atttcaatta cggaaagact
    16021 cttttttaag caaagaatag aaatatgaat aaaaataatg ggctaccaaa agtaacccat
    16081 tgacaaaaaa tgcggcgata ccggaaggta tcgccaacac atttattgct tagtaaacgg
    16141 cgcgaagatt tcgctaaatg cgtacttcgg tgcctgttct ttagtcaggc cgtggaattc
    16201 cggagacagg ttagtgccgc ccggcaggtc gcgcgcgatt gaccagatac caaccatacc
    16261 gagattacgc ttctgcgcat cctgcatgac cagacgagca tcagagagat agaacacctc
    16321 gccctgaacg tcgttcacgc caaccatcgg cgtggtaccc atcatagcgt caatttctgc
    16381 atcgctcttg ttgggatgga ggcctttcaa ttgtgaatgc aggttggcaa tcgcagacgt
    16441 tgcacactta ccgtgaatgt tctggccttc ggtatttgca gactgacaaa tcgcgttacc
    16501 gtagtccatt gtcatcacgt tcacacccgc cagctcaaca cctttcgctt tggcatcgct
    16561 caggacattc atcccttccg gcgtcaggcc agtcggtaga attggcaagg tgtaccagat
    16621 agcaatatct ttgccttctg acttccattt atcctgcact ttcttcacag caaggttacg
    16681 acgttcaata gatgcctgat ccgcaaccca ggtgccttcg atatcgaagt ccaggactct
    16741 gaggttcagg ttatcaacga tgtcataata atgctgcatc agatcgtcta cgttcttaca
    16801 ggaagcagcc agcggagcgt tgttagcacc accgatagac agcatcacat cgccgccagc
    16861 ctcacgcaga gctttgattt tgctgtactg agcgtaattc tgcataccat aagcggtacc
    16921 ccatgtcggc agacaggtgt tcgcatcttt actcaccaca aacgccagcg tgaagtggtt
    16981 gacgttatga ttcttagcca gcgcagccag atccgggatc gtattcagcg taaagtcaac
    17041 atacggtgca taaacgtgtt tcggccatgc tttcttcggc gtacctacac gttctttggt
    17101 tccggtccag tcaacaaaaa cacgccacgg gttgctgtca gaaggagaaa ctttctgcac
    17161 tttactctga gaaatgtagt tcacaccgtt aaagcgaatc agcgtatcgc tggcataaag
    17221 cgtttctgga ttaaactgcg gcgcattatt cagctcttcg ttgctatagc tctgagcctt
    17281 acctaacggc ttccacgggc ggctattgct accggtggcg ttttggttcg caaccagagc
    17341 agggttgtca ctttgcgtcc agaacagcgc ttcataagcc tgcccatcaa caacaacgcg
    17401 atcacctttc acgtacacag tggatgcgga ccatgctttc gccggttcgt atttcttcca
    17461 cgggttatcg ccgcccggga caaagcccca tgcgtcttga tcgacagaag ttttataatg
    17521 tgcatcgtta taatctacga tgctgtccgc gctgtacgct ttgctggcct ggaaaccatc
    17581 cgcaacaatg acgccgccgt tatcaatttc acaggtagtc gggttaccgt actgactcaa
    17641 ttccgcagcg gtagctgtac gctcgagacg ccatgggtta ttggagtttt cagcgctggt
    17701 accccgtggg caatctttag acccaaccca ccaggcgttc ttgtaaacat taccattgaa
    17761 aataacatgg taaatttcgc taccttccta gccgctccac gcttgcaatg agtaatcgtt
    17821 accgctatcc gctggtgttg atggctcgct gttatccgga gttggggtca ccggtgtttc
    17881 cggagttgga tcaaccggag tcacagcacc gccattatta tccggcttaa cggaacagga
    17941 gccagggtta ccatactggc tgatttctgt tgccgttgcc gcgcgaacat aacgccatgg
    18001 gttactggca tcgttttctt tcgcatcgcc tgggcaattt gccgcgccta cccaccaggc
    18061 gttcttataa atgccgccgt tatagataac gtaccatgta gaagaacctt cctgaccttt
    18121 ccaagctact acagcatagt tgtcgtttga cttatcagct ggggctggct gaacatcagg
    18181 agccggttta acgtccggtg ccggtttcac atccggtgca ggctgtggtg ctgaagtaca
    18241 ggactgtgga ttactggttt ctgagatttc cgttgccgta gcggcacgaa cataacgcca
    18301 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
    18361 ggcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
    18421 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
    18481 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
    18541 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
    18601 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaga
    18661 aacccaccag gcattttcat aaattttgcc atcgaaaata acctgatatt tattaccacc
    18721 ttgttggtta ttccatgctt ccattgccaa tactggcaga gcggaacaca ccagccccat
    18781 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
    18841 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
    18901 ttaaagtgcg gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
    18961 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
    19021 tctgacagta cgaattagat atacagccaa caaatgcaaa aagggcgccg aagcgccctt
    19081 tttaattcga aactaattaa cgtgtaatta gcccagaact ttagcaacaa cgcccgcgcc
    19141 aacggtacgg ccgccttcac ggattgcgaa acgcagaccg tcgtccatcg cgatcgggtg
    19201 gatcagggta acaaccattt tgatgttgtc gcccggcatt accatctcta cgccttccgg
    19261 cagttcgatg gtaccagtca cgtcagtagt acggaagtag aactgcggac ggtagccttt
    19321 gaagaacgga gtatgacg
//
LOCUS       DACAPB010000078        18909 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.078, whole genome shotgun sequence.
ACCESSION   DACAPB010000078 DACAPB010000000
VERSION     DACAPB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.078"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(203..259)
                     /locus_tag="IT904_002469"
     CDS             complement(203..259)
                     /locus_tag="IT904_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP3192237.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            538..1785
                     /gene="mdtA"
                     /locus_tag="IT904_002470"
     CDS             538..1785
                     /gene="mdtA"
                     /locus_tag="IT904_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAP3192238.1"
                     /translation="MKGSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            1785..4907
                     /gene="mdtB"
                     /locus_tag="IT904_002471"
     CDS             1785..4907
                     /gene="mdtB"
                     /locus_tag="IT904_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAP3192239.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSCLGTWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            4908..7985
                     /gene="mdtC"
                     /locus_tag="IT904_002472"
     CDS             4908..7985
                     /gene="mdtC"
                     /locus_tag="IT904_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAP3192240.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7986..9401
                     /locus_tag="IT904_002473"
     CDS             7986..9401
                     /locus_tag="IT904_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192241.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINTPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9398..10801
                     /gene="baeS"
                     /locus_tag="IT904_002474"
     CDS             9398..10801
                     /gene="baeS"
                     /locus_tag="IT904_002474"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAP3192242.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALSEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10798..11520
                     /gene="baeR"
                     /locus_tag="IT904_002475"
     CDS             10798..11520
                     /gene="baeR"
                     /locus_tag="IT904_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAP3192243.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11711..12043
                     /locus_tag="IT904_002476"
     CDS             11711..12043
                     /locus_tag="IT904_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP3192244.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12190..13551
                     /locus_tag="IT904_002477"
     CDS             12190..13551
                     /locus_tag="IT904_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAP3192245.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13882..14199)
                     /locus_tag="IT904_002478"
     CDS             complement(13882..14199)
                     /locus_tag="IT904_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192246.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            14605..15504
                     /gene="yegS"
                     /locus_tag="IT904_002479"
     CDS             14605..15504
                     /gene="yegS"
                     /locus_tag="IT904_002479"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAP3192247.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     SVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(15586..16365)
                     /locus_tag="IT904_002480"
     CDS             complement(15586..16365)
                     /locus_tag="IT904_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP3192248.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(16465..17505)
                     /gene="gatD"
                     /locus_tag="IT904_002481"
     CDS             complement(16465..17505)
                     /gene="gatD"
                     /locus_tag="IT904_002481"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP3192249.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(17553..18908)
                     /locus_tag="IT904_002482"
     CDS             complement(17553..18908)
                     /locus_tag="IT904_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HAP3192250.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
BASE COUNT         4429 a         5068 c         4913 g         4499 t
ORIGIN      
        1 cggcctacct cctttcgcca tgcaagcagt ctatcgctaa cttgcagata aaatagtttc
       61 acatgtgatt attgcgtgac gtctcgcaaa gaatatttgc ttgcaggatc gcagactgca
      121 aagcctgcgg attgacaatc ggaccgtgaa ggcatacttt caggagtgag ggtagagcgg
      181 ggtttccccc gccctggtag tcttagtaag ctgggtagct aacgactaag agcaccacga
      241 tgatgagtat cttcatcatg accctttcct tatttaaggc cccttcctcg ggaggggctt
      301 tcccgtttca gcgtcccgct gaaatcgtcg gcctacctcc ttttgccctg aatgcagtct
      361 atcgctaacg cgtagataaa atagtttcct gtgttattac tggatgggtg ctcgcaaatg
      421 tgcccgccat tcagacgatt cccgatagtg tttcataatt cctccatttt tctccctgat
      481 tggctggcta cactagtatc attccgcgaa acgtttcagg aagagaaact cttaacgatg
      541 aaaggcagtt ataaatcccg ttgggtaatg gtaatcgtgg tggttatcgc cgccatcgcc
      601 gcattctggt tctggcaagg ccgcaatgac tcccggagtg cagcgccagg ggcgacgaaa
      661 caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc gttccggccc attagccccg
      721 gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt acctcaccgg gcttggcacc
      781 attaccgccg ctaatactgt tacggtgcgc agccgcgtgg acggtcaact gatggcgttg
      841 catttccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc
      901 cagttcaaag ttgcattagc acaagcccag ggccaactgg caaaagataa agccacgctt
      961 gccaacgccc gccgtgacct ggcgcgttat caacaactgg taaaaaccaa tctcgtatct
     1021 cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct
     1081 gatgaagcaa gcgtcgccag cgcgcagctg caactcgact ggagccgcat caccgcacca
     1141 gtcgatggtc gcgttggtct caagcaggtt gatgttggta accaaatctc cagtggtgat
     1201 accaccggga ttgtggtgat cacccagacg catcctatcg atttgctctt taccctgccg
     1261 gaaagcgata tcgctaccgt agtgcaggcg caaaaagccg gaaaaccgct ggttgttgaa
     1321 gcctgggatc gcaccaattc aaagaaatta agtgaaggca cgctgttaag tctcgataac
     1381 caaatcgatg ccactaccgg tacgattaaa gtgaaagcac gcttcaataa tcaggatgat
     1441 gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacacaga gcaaaacgct
     1501 gtggtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg
     1561 aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa
     1621 gtggtgatcc gcgcaggtat ttctgcgggc gaccgcgtag tgacagacgg cattgatcgc
     1681 ctgaccgaag gggcgaaagt ggaagtagtg gaagcccaga gcgccaccac tccggaagag
     1741 aaagctacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc
     1801 cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc
     1861 tgatggtggc gatcttactc gccgggatta tcggttatcg agccctgccc gtttcggcgc
     1921 tgccggaagt ggactatccg accattcagg tagtcacgct ctacccaggt gcgagcccgg
     1981 atgtcatgac ctctgccgtt accgcgccgc ttgaacgcca gtttgggcag atgtctggcc
     2041 tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc
     2101 taacattacc gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca
     2161 acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc
     2221 cgccgatcat gacgctcgct gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata
     2281 tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacac
     2341 tttccggcgg ccagcgtccg gctgttcgtg tcaaacttaa cgctcaggcg attgccgccc
     2401 tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtc aactcggcaa
     2461 aaggtagcct cgacggccct tctcgtgcgg ttacgctttc cgcgaacgac cagatgcaat
     2521 ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcaccg attcgtctgg
     2581 gcgatgtcgc aaccgtagag caaggtgcag aaaacagctg tcttggcaca tgggctaaca
     2641 aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg
     2701 ccgacagcat ccggcagatg ctgccacagc tcaccgagag tctgccgaaa tcggtgaagg
     2761 tgacagtact ttctgaccgt accaccaata ttcgcgcatc cgtggatgat actcagtttg
     2821 aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgtaatattc
     2881 cggcgaccat cattcccggc gtcgcagtgc cgctgtcgtt aatcggcacc ttcgcggtca
     2941 tggtgtttct cgatttttca atcaataacc tgacgctgat ggcgttaact atcgccaccg
     3001 gatttgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag
     3061 gcgaaaaacc gctggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct
     3121 cgctgacctt ctcactgatt gcagtgctga tcccgctact gtttatgggc gatatcgtcg
     3181 ggcgactgtt ccgcgagttt gccattaccc tggcggtagc aattttgatc tcagccgtgg
     3241 tgtcgctgac tctgacgccg atgatgtgcg cgcggatgct cagccaggag tcgctgcgta
     3301 aacagaaccg cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg
     3361 gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg gcactcagca
     3421 cgctgctgct tagtgttctc ttgtgggtgt tcattccgaa aggtttcttc ccggtacagg
     3481 acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg
     3541 cccagcggca acgccaggtc gcggatgtga ttttgcaaga tccggcggtg caaagtctga
     3601 cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa cagtgcgcgt ttgcagatca
     3661 acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa
     3721 cggcggtgga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gatctgacca
     3781 tcgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg
     3841 cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcagttgcca cagctttctg
     3901 atgtctccag cgactggcag gaccaagggc tggtggcgta tgtcaatgtt gatcgcgaca
     3961 gcgccagccg tctggggatc agcatggcgg acgtcgataa cgccctttac aacgcgtttg
     4021 gtcagcgact gatttccact atttatactc aggccaacca gtatcgcgtg gtgctggagc
     4081 acaacaccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg
     4141 atggtggcgt ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgctct
     4201 ccatcaacca tctggatcag ttcccggtaa cgaccatctc ctttaacgtg ccggataact
     4261 attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg
     4321 tggatatcac cacgcagttc cagggcagca ccctcgcctt ccagtcggcg ctgggcagca
     4381 ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg
     4441 agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg gttggcgcac
     4501 tgctggcgtt gctgattgct ggtagcgaac tggatgtgat tgcgattatc ggcattattt
     4561 tgctgatcgg tatcgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgccg
     4621 agcgcgagca aggcatgtcg ccgcgcgagg caatctacca ggcttgtctg ttgcgttttc
     4681 gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta
     4741 ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggcctgattg
     4801 tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg
     4861 cattgtggac caaaagccgc tttgcccgtc atgaagagga ggcgtaagtg aagttttttg
     4921 ccctcttcat ttaccgtccg gtggcgacga ttttactgtc ggttgccatt accctgtgcg
     4981 gcatactggg cttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga
     5041 ttatggtcag cgcctcgctg cccggtgcgt caccagaaac aatggcgtct tccgttgcca
     5101 cgccgctgga gcgctcactt gggcgcattg ccggagtcag tgaaatgacc tccagcagtt
     5161 cgctcggcag cacgcgtatt attttgcagt ttgattttga ccgggatatc aacggcgcag
     5221 cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagt gggatgccca
     5281 gccgcccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctcacgctga
     5341 cgtccgatac ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag ctggctccga
     5401 cgatttcgca aatcgacggt gttggtgatg tcgatgtcgg aggcagctca ctgcccgccg
     5461 tacgcgtcgg gctgaatccg caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac
     5521 gcaccgccgt cagcaatgcc aacgtgcgta aaccgcaggg cgcgctggaa gatggcactc
     5581 accgctggca gatccagacc aatgatgagc taaaaaccgc cgctgaatat cagccgttga
     5641 ttattcacta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgaccgact
     5701 cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttactgatga
     5761 tccgcaaact gccggaagcc aatattatcc agacggttga cagcatccgg gcaaaattac
     5821 cggagttgca ggaaaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc
     5881 ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg
     5941 tgattctggt ggtgttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttt
     6001 cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca
     6061 ataacctttc gttaatggcg ctcaccatcg ctactggttt cgtggtggat gacgccatcg
     6121 tggtgctgga aaacattgcc cgtcatctgg aagcgggaat gaaaccgttg caagccgcac
     6181 tgcaaggtac tcgcgaagtc ggttttacgg tgctgtcgat gagtctgtca ctggtggcgg
     6241 tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc gaatttgccg
     6301 tgacgctttc tgtcgccatt ggtatatcgt tgctggtttc tctgacatta acgccaatga
     6361 tgtgtggctg gatgctgaaa gccagcaagc cgcgcgagca aaagcgactg cgtggttttg
     6421 gtcgcatgtt ggtagccctg caacaaggct acggcaagtc actgaaatgg gtgctcaatc
     6481 atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tggctgtata
     6541 tctcgatccc gaaaaccttc ttcccggagc aggacactgg cgtgttgatg ggcgggattc
     6601 aggcggatca gagtatttcg tttcaggcga tgcgcggtaa gttgcaggat ttcatgaaaa
     6661 ttatccgtga cgatccggca gtggataatg tcaccggctt tacaggcggt tcgcgagtga
     6721 acagcgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa acggcgcagc
     6781 aaattatcga ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat ctgttcctga
     6841 tggcggtaca ggatattcgc gttggtgggc gtcagtcgaa cgccagctac cagtacacgt
     6901 tgttatccga cgacctggcg gcactgcgag aatgggagcc gaaaatccgc aaaaaactgg
     6961 cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc gcggagatga
     7021 atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtacaa gccgccaaca
     7081 gtctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag ccgatgaacc
     7141 agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt gcgctggaaa
     7201 aaatgttcgt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc gctaaatggc
     7261 aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc tcgaccattt
     7321 cgtttaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc gatcgcgcaa
     7381 tgacccagct tggtgtgcct tcgacggtgc gcggcagttt tgccggcacg gcgcaggtgt
     7441 tccaggagac gatgaactcg caggtgatcc tgattatcgc cgccatcgcc acggtgtata
     7501 tcgtgctggg tatcctttac gagagttacg tacatccgct gacgattctc tccaccctgc
     7561 cctcggcggg cgttggagcg ctgttggcgc tggagctgtt caatgccccg ttcagcctaa
     7621 tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc attatgatgg
     7681 tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa gctattttcc
     7741 aggcctgtct gctgcgtttt cgcccgatta tgatgactac cctggcggcg ctgtttggtg
     7801 cgctgccgct ggtattgtcg ggcggcgacg gctcggagct gcggcacccc ctggggatca
     7861 ccattgtcgg cggactggta atgagccagc tccttacgct gtataccacg ccggtggtgt
     7921 atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa acggtaaccg
     7981 agtaaatgac agatcttccc gacagcaccc gttggcaatt gtggattgtg gctttcggct
     8041 tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca atggcgcaaa
     8101 gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg ctgaccgtgg
     8161 cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc aatattttct
     8221 ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc ggcacgctga
     8281 acgaactgtt gctggcacgc gcgttacagg gcgttggcgg cgcgatgatg gtgccggtcg
     8341 gcagattgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg atgacctttg
     8401 tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt ctgctggtgg
     8461 agtacgcatc gtggcactgg atctttttga tcaacactcc ggtggggatt atcggtgcga
     8521 tcgccacatt gctgttaatg ccaaactaca ccatgcagac gcggcgcttt gatctctccg
     8581 gatttttatt gctggcggtt ggcatggcgg tattaaccct ggcgctggac ggcagtaaag
     8641 gtacaggttt atcgccgctg acgattgcag gcctggtcgc agttggcgtg gtggcactgg
     8701 tgctttatct gctgcacgcc agaaataaca accgtgccct gttcagtctg aaactgttcc
     8761 gtactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt ggcagtggca
     8821 tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg ccgtttcatg
     8881 ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga attgtggtac
     8941 aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg ggtctgtcgc
     9001 tggtcaccct gttgtttatg actaccgccc tgctgggctg gtactacgtt ttgccgttcg
     9061 tcctgttttt acaagggatg gtcaactcga cgcgtttctc ctccatgaac accctgacgc
     9121 tgaaagatct cccggacaat ctggcgagca gcggcaacag cctgctgtcg atgattatgc
     9181 aattgtcgat gagtatcggc gtcactatcg ccgggctgtt gctgggactt tttggttcac
     9241 agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc tggcttagca
     9301 tggcgttgat catcgccctt ccggcgttca tctttgccag agtgccgaac gatacgcatc
     9361 aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc gacccggtat
     9421 taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga tcagtatgca
     9481 ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc atggtaatga
     9541 acagcgatta caactgttaa gtgatgcgct tagcgagcag tatgcgcagc atggcaactg
     9601 gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat ttgaacacga
     9661 taattcggaa gataaacccg gcccgggtat gccaccgcac ggctggcgta cccagttctg
     9721 ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc cacctgacgg
     9781 tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg cctcccccgt
     9841 tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc aaaccagctg
     9901 gttgattgtc gccctggcaa cgttactcgc ggcacttgcc acttttctgc tggcgcgcgg
     9961 tttactggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg cgggcgattt
    10021 cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc aagacttcaa
    10081 ccagcttgcc agcacactgg agaaaaacca gcaaatgcgg cgcgatttta tggccgatat
    10141 ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag ccattcagga
    10201 tggcgtgcgt aaattcacgc cggagacggt ggcgtcttta caggcggagg tcggtacact
    10261 gaccaaactg gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg ctctcgccta
    10321 tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg catttcgcga
    10381 acgattcgcc agtcgtggcc tgaaactgca attttccctg ccagacagta ttaccgtatt
    10441 tggcgatcgc gaccgtttaa tgcagttatt caataactta ctggaaaaca gcctgcgcta
    10501 cactgacagc ggcggcagcc tgcaaatctc tgccgggcag cgcgacaaaa cggtgcgcct
    10561 gacctttgcc gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat tgtttgaacg
    10621 tttttatcgc accgaaggtt cccgcaaccg tgccagcggc ggttccgggc tggggctggc
    10681 gatttgcctg aacattgttg aagcacataa tggtcgcatt attgctgccc attcgccttt
    10741 tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga gagaagtatg
    10801 accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga tgaaccgaag
    10861 ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac gcttatcagc
    10921 cacggcgatc aggtactgcc gtatgtgcgc cagacaccac cggatctgat cctgttagat
    10981 ctgatgctcc ctggcaccga tggcctgacg ctgtgccggg aaattcgtcg tttttctgac
    11041 attccgatcg tgatggtgac ggcaaaaatc gaagagatcg atcgcctgct ggggctggag
    11101 attggcgcag atgattatat ctgtaagccg tacagcccac gggaagtggt agcgcgcgtc
    11161 aaaaccattt tgcgccgttg caaaccgcag cgcgagttgc agcaacagga tgctgaaagc
    11221 ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat gcttgacctg
    11281 acgcctgcgg aatttcgtct gctgaaaacg ctctctcacg aaccaggaaa agtgttctcc
    11341 cgcgagcaat tgctcaatca tctttatgac gactaccgcg tagtaaccga ccgcaccatc
    11401 gacagccaca ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga acagtcattt
    11461 atccgcgccg tttatggcgt cggttaccgc tgggaagccg acgcctgccg catcgtttag
    11521 ttttagcgac attattttgt tagccggaga tggcgttcag accagatccg gcaacattat
    11581 cccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc agaaaatcgc
    11641 tcatacttta atcggtaaac agcaccttta gatgctgttt tcgatacaca atttcaatca
    11701 aggagtcatt atggctggtt ggtttgaact cagtaagagc agtgataatc agttccggtt
    11761 tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctttata cctcaaaagc
    11821 ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga
    11881 gaaaaaaacg gcaagtaacg gcaaattcta tttcaatctg aaagccgcta atcatcaaat
    11941 tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt
    12001 taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat gccgggcgtg
    12061 ggttgttggc gtccggcgct ttaccagatc cccccacagc gctacaatgc ccgcccttaa
    12121 agtgggggca ctcccctaac cgcttcatca ggtgaagcgg atctgacctg tcatcagaac
    12181 gagagaatta tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt
    12241 tacgctttcg cttatggcgc agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt
    12301 gtgcgcaaca acgaattcaa ccacgaaaat cttcagctcg gcatcaatga agcccacgcg
    12361 ctggggaaaa agttttatgt cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc
    12421 tttatccgtg acctgaaacc ggtggtggaa atggggccgg atgcgctgat tatgtccgat
    12481 ccagggctga ttatgctggt gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg
    12541 caggctaacg ccgtaaactg ggcgacggtg aaattctggc agcaaatggg cctgacccgc
    12601 gtgatcctct ctcgcgagct gtcgctggaa gagattgaag agatccgcaa tcaggtgccg
    12661 gatatggaga tcgagatctt cgttcacggc gcactgtgca tggcctactc cggtcgctgc
    12721 ctgctctctg gctatatcaa caagcgcgat ccgaaccagg gtacctgcac caacgcctgc
    12781 cgctgggagt acaacgtcca ggaagggaaa gaagatgacg ttggcaacat cgtacacaag
    12841 tacgagccga ttccggtgca aaatgttgag ccgacgctgg gtatcggcgc gccaaccgac
    12901 aaagtgttta tgatcgaaga agcccagcgt ccgggcgagt atatgaccgc gtttgaagat
    12961 gagcacggca cttacatcat gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc
    13021 ctgaccaaaa tgggcgtgca ttcgctgaaa atcgaaggcc gtactaaatc tttctactat
    13081 tgcgcacgca ccgcacaggt ttaccgtaaa gctatcgatg acgccgctgc gggcaaaccg
    13141 ttcgatacca gcctgctgga aactctggaa ggtctggcgc atcgtggcta taccgaaggt
    13201 ttcctgcgtc gtcatactca cgacgattat cagaactacg aatacggtta ttcagtttct
    13261 gaccgccagc agtttgttgg tgagtttacc ggtgagcgca agggggacct cgcggcggta
    13321 gcggtgaaaa ataaattctc cgttggcgac agccttgagc tgatgacgcc gcaaggcaac
    13381 attaacttta cccttgagca catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca
    13441 ggcgatggtt atactgtgtg gctcccggtc ccgcaggatc ttgagctcaa ttacgctctg
    13501 ctgatgcgta atttctccgg ggaaaccacg cgtaacccac acggtaagtg attaatttcg
    13561 attatttttc ccggatggaa aattcttaga aaccgatcac atacagctgc atttattaag
    13621 gttatcatcc gtttcgctga aaaacataac ccataaaatg ctagctgtac caggaaccac
    13681 ctccttagcc tgtgtaatct cccttacacg ggcttatttt tttatgcata agccctatct
    13741 ctggtaaccg tcttccattg accacatcga tagaatcctc cttcatagca cgatgccttt
    13801 cacttatcgg catcgtgctc ccacaggttc cggctacgca cagccagaac gcgcatattt
    13861 gacgcttacc aaaaaatatt ctcactctcc acatttgaat gtcagacgag cgacaccatg
    13921 taatcctaca ccttctgtct tcagctcaac tatttgcatt tttttgccct gagtaacaca
    13981 gaaatgagtt gcatcatttt ttactatatt ttctgcacca gatattctac ccctggctaa
    14041 agaagcttcg gcttcggtgt agtattggtt atcgagttta cgctgaatat tacttttata
    14101 tgcaagacca aatttaccga tacttgtctc atcattatgc acagcacaac cagacataat
    14161 aaaaatacta attaatgata tagcagctat ctttttcatc tcaccttccc ccattaaata
    14221 ccaacgacac tctctagtgt ttaaatataa taatggcatg attattataa ttgaatagga
    14281 ttataataaa tgttctgtac aacatttcct acataagtag gaattacgga cattgaggcc
    14341 cttcagggta actccatggg ggctttaata tattatattg aagatgccac tgcttagttg
    14401 aatattaggt atatgctctt ttttgaaatt tatcggtggc agccgttagt attcgctgtc
    14461 cccactgcaa gctcctggtg gtaaccactg aatcctccat acttgaactg actttttatc
    14521 ctccgacttt catcctgttc tgactccacc ttttgttttc tgctctacac tatctacaga
    14581 ccaatcataa aggcacatac gatcatggca gaatttcccg ccagcttact gattcttaat
    14641 ggcaaaagta ctgacaatct acccttgcgc gaagcaatta tgctgttgcg tgaggaagga
    14701 atgacgatcc atgtgcgggt cacctgggag aaaggcgatg ccgcacgata tgtagaggag
    14761 gcccggaagt tgggcgtcgc aacggtgatt gccggtggtg gcgatggcac cattaatgaa
    14821 gtttctacgg cgttgattca gtgtgagggg gatgacatac ccgcgctggg aattttgcca
    14881 ttaggaaccg ccaatgattt tgccaccagt gtagggattc ctgaggcact ggataaggcg
    14941 ctgaaactgg caattgccgg tgacgccatt gcgatagata tggcgcaggt caacaaacaa
    15001 acctgtttta ttaatatggc gacaggcgga tttgggacgc gtattaccac agaaacgccg
    15061 gaaaaattaa aagccgcgct gggtagcgtc tcttacatca ttcatggctt aatgcgtatg
    15121 gatactctgc aaccggaccg ttgtgaaatc cgcggtgaaa actttcactg gcaaggtgac
    15181 gccctggtca ttggtattgg taacgggcgt caggccggtg gcggtcagca attgtgtccg
    15241 aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt ttaccggcga tgaaatactt
    15301 ccggctctcg tatcaacctt aaaatctgac gaagataacc cgaatattat cgaaggcgct
    15361 tcgtcgtggt ttgatattca ggcaccacac gacatcacct ttaatcttga tggcgaaccg
    15421 ttgagtgggc aaaattttca tattgaaata cttccggcag cgttgcgttg tcgattacca
    15481 ccagattgtc cactgttgcg ttaatcaaac tattctctct tgaaccaggg agaatccgta
    15541 atgccggatg cggtgtaaac accttatccg gcatacagaa caatactaca ccgtaattaa
    15601 ttttacccgc gtcttctgca atgccagttt atccacctct gacacagcat catcggtaat
    15661 aataatgtca attttttcga ttggtagtac ctgattaaaa ccgcgacggt taaatttcga
    15721 tgaatcgaga acggcaatca ctttattagc ggctgtgacc atcgcaccgc taatagaata
    15781 accttcatta aaggtcgtaa taccattaac cgcatcaata ccatcagcac cgacaaacat
    15841 taaatcggca ttaatatcct gtaatgaacg ctcggcaatg gaaccatgca tcgagcgcgt
    15901 tttatggcgg accgtgccac cacagacgac cagagtaatg tctttatttt cggaaagggc
    15961 aaacgctgcc gggagactgt tggtaatcac cgtgatattt ttggcggtca ttaatccttc
    16021 ggcgataagc attgtcgtac ttccgctgtc gagaataacg gtcatccctt cgtggatcat
    16081 tttgactgcc gcctgcgcta ttctgttttt tggcgcgctg gcgagctgaa atcgctcttt
    16141 aaacccgact tcctgggtct cggtttcact attaccagac attattttcg ccgcaccgcc
    16201 atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc aaatcggcac ggattgtcgc
    16261 ttccgaggca gcaaatactc ccgccagatc ctgaacaagc acggttccct gttcattcac
    16321 taattggatg atcttattcc ttcgctcgaa tgaattcatc gtcatttacc catattcaat
    16381 tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat ctgttttgca caataccggg
    16441 ccagcatcac gctggcccgc ggtttcaggg aatgagcaac actttgcccg gcatagcatt
    16501 acgagcgatg tcacgcaccg cctgggcgaa gctttcaaag cttccacggt gagcgattaa
    16561 tggctccagg cttaacttac gttctgtcag caaccggctc gccgtttccc actcctgccc
    16621 cggccaaggg ctggagtagt tcatccaact gccgataacc gtcagctctt tacgcaatat
    16681 tttgccaaac gttgccgatg ttaaatgcag atcctgatgc aacgtgccca ccagcgccag
    16741 ttgggcatga ggaccggcaa tctctaccgc cagttcgaca gtttgcggta cgccagccgt
    16801 ctcgaggata agctgattaa agcgcagttc gcgtaaaacg ctctgcattt gcggcgcgct
    16861 catttcgcta ctgttaaatg tttgcatcgc accgaaagat tttgccagtg ccagtttttc
    16921 tgaactaatg tcgatcgccg tcacactctt tgctcccagc gcgacagcgc actgaatggc
    16981 cagcaggcca atggttccgg caccaataat aataacgttt ttattctcac aaccttgcgc
    17041 taaatgaaaa gcatgcagac caacggtaat cggctcaata aaagccccat cctcaatagg
    17101 catatccgtg ggtagagcaa agacattttt tcgcttaacg acaatatatt cagcaaatcc
    17161 accatcacgc cgcgagccaa taaaatcata ttttgcgcac tgggaataaa accctttcag
    17221 acactctgga caagtaaaac agggtaataa cggcacacag gcaaccgcat cgccagggtg
    17281 taaatcatca acaccggatc ccaccgcatc aatatagccg ctaaattcat ggcctaacgt
    17341 tattggataa taatgtgcac catttttaaa tatcctgggt aaatcggaac cacataagcc
    17401 cgagctggca atttttaccc gcacctcatc ctgatgttta atttcaggaa tgacgctttc
    17461 tgcaacgcgc acgataccat cagtatcatt caccactgat ttcataaaaa ctcctgattg
    17521 ttaaggggga taacctcccc ctcaggtaaa aattattctg cgagaacgac tttctcttgt
    17581 ttaataaagc cacgcgctct acgccaggtc atgaaaatac cggtcagata aattgcaccg
    17641 ataataatga aaccgggaat attttgcggg gagaaaacct gaatcagtaa ccaggtaatg
    17701 ggagaaccgc cctgatccat tgaagccacc atacccccgg cttttaacgc cccagcatta
    17761 gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga tccacagggt gatgctcata
    17821 atgatgacac ccgagattaa ggtgcggaac agatttccac gatgcacggc gaccgccatc
    17881 gccacgaaga agccgatggt ggcaagatcg ccaaacggca gcacctgatt acccggcaca
    17941 caaacagcaa ttaaaatggt gagtgggata aaaatcaggc ttgccgatac caccgccgta
    18001 tgtcccagca gcaacgccgg atcaaggcca atcaggaact cctgaccgcc gaacttcgcc
    18061 tgtaaacgac tacgagcctg cttagcgatg ggcgttaaac catccatgat gggtttaatc
    18121 acccgtggca ttagcagcat cactgccgcc gtttttaccg ccagctgcaa tacacctttg
    18181 acatcgtaac ccgcgaggat gccgataatc agccccatca caaaacccac ggtgacaggc
    18241 tcgccaaatg gaccaaattt gcgctgaata tcgtcggcgc taaatttaat tcggttaacg
    18301 cctgggattt tctcgatgat agcatcgacc agcaccgcaa tcggccccat atacgccgac
    18361 gtaccgtgcg gaatagcaat accttccagc tcaaagaaat ttcgggtatc gcgggcaaac
    18421 cagtcgccga gcttataaac aaacgccgcg tgaattacca cacctgccat ccctatcatc
    18481 catgaaccgg ttgccagatg cagcaacgcg ccggtgaagg tcatatgcca gatattccag
    18541 atatcaacat ttaccacccg cgtcatacgg gtcagtaaca tggccacgtt aaccagaatc
    18601 gcaatcggaa tcgccaccag cgcaatttgc gacgcccagg tcattggtga agagcccggc
    18661 cagccaacat cgaccacatg cagattcagg tcgaaatttt ccgccatcgc tttcgccgcc
    18721 gggccaatgg aatccagcat taagccaatc acaaggccaa tgccaacaaa gccaatcccg
    18781 atatgcagac ccgctttaaa gcaatcgcct gccttcatgc ctaatatttt agaaaaaata
    18841 atgatgacaa tcggcagcat caccgtaggg ccgaggtcga gaatataacg catgacttct
    18901 gaaaacata
//
LOCUS       DACAPB010000079        18836 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.079, whole genome shotgun sequence.
ACCESSION   DACAPB010000079 DACAPB010000000
VERSION     DACAPB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.079"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(36..2168)
                     /locus_tag="IT904_002483"
     CDS             complement(36..2168)
                     /locus_tag="IT904_002483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAP3192251.1"
                     /translation="MNRAQAQQLLQTALANPAAEFRDGQWEAIDALVNHRQKLLVVQR
                     TGWGKSSVYFISTKIFRDRGMGPTIIVSPLLALMRNQIESAARLGIVAETMNSTNTDD
                     WRSVTQRILNNQIDCLLISPERLANDSFIETVLQPIADRIALMVIDEAHCISDWGHDF
                     RPDYRRIVNILRQLPANTPVLGTTATANNRVVADIQTQLGNIQIHRGPLIRESLSLQT
                     MVLPDQASRLAWLAQVIPTLPGTGIVYVLTQRDADLVAKWLNQQGISALAYYSGVTHP
                     NFVNYAGKEDTDLYRQFLETQLLENKIKVLVATTALGMGYDKPDLGFVLHYQAPSSIV
                     AYYQQVGRAGRGIENAIGVLMSGTEDNDIHAFFRRTAFPSQDDVQRLLAALEDADGLS
                     LEKLEKEANLPQKRIEHVLKYLRTESPSPVIAIGKTWFRAPVRYQMDLEHIRKITEIR
                     EREWSEINDYLNTSGCLMLALRNALDDPSQETCGKCENCIAEPVLTKVFDQEIGIRAT
                     QFLRHSEEIIKPRKQIAASNDEAARTFVEYEFPRNLGDLVAQEGRVLSRWGDAGWGRT
                     VADNKHAGRFSDELVEAMAEMIQQRWQPNPAPQWVCCVPSRNHPELVPDFARRLAARL
                     GLPFVDAVSKVKDNQPQKGQQNRFHQCRNLDGAFAVTQLYQGQPVLLVDDIVDSGWTL
                     TVVAALLQQAGSGVVYPIALASSSVKDS"
     gene            complement(2183..3337)
                     /locus_tag="IT904_002484"
     CDS             complement(2183..3337)
                     /locus_tag="IT904_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP3192252.1"
                     /translation="MSWQSEPLSALTLKIGSGSTPRGGDSVYISEGTTLIRSQNVYNN
                     EFSTDGLVYIDEKTAEKMKGVSVEKEDVLVNITGDSVARCCLVHDAVLPARVNQHVAI
                     LRTNPKKLLPHFLAFYMVSPFMQAKMLSWAGTGGTRKALTKGMLEGFEIPLPPVDVQD
                     KIVQKIKTYNDLIENNRRRIQLLEESARLLYQEWFVHLRFPGHKQVKITDGVPEGWSK
                     EPLENLLVLQRGFDLPVSKRIEGSVPIYASTGINGFHNVAKVKGPGVVTGRSGSLGTV
                     MYVAKDYWPLNTTLWVKEFKKASPIFATYLLRAMKLEGYNGGAAVPTLNRNDVHKVDV
                     LCPESKLMNEFEVQVENIFKQIDKLKEYNEKLAQARDLLLPKLMSGELTV"
     gene            complement(3334..5475)
                     /locus_tag="IT904_002485"
     CDS             complement(3334..5475)
                     /locus_tag="IT904_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAP3192253.1"
                     /translation="MAQLENIEAIEKRLWKSADTLRANSELASNEYFLPVMGLIFLRH
                     AYSRYLSLKDEIIANLPSRGGKTRELTKEDFSKKSAIYLKPEAQFDYLIALTDADNRA
                     EAIIHAMDSIETDYTNLRNQLPKQEYNNIPNDVLGMLLRTLNPEELKKATGDIFGRIY
                     EYFLTQFADQGAHDGGEFFTPVSLVQLIVNVLEPDHGKIFDPACGSGGMFVQSAHFME
                     RHAQDPHELTFYGHEKNRVTTRLAKMNLAVHGLEGNVEGGEAAITYYNDPHEGLFGTV
                     DYVMANPPFNVDEVDADKIKGDKRRLPFGLPGVNKNKKVSNANYLWIQYFYSYLNDSG
                     RAGFVMSSQASSAGRDEAKVREQLVKTGHVDIMVDIRGNFFYTRTVPCQLWFLNKNKP
                     AHLKDKVLMLDARNVYRKVTRKIYDFSPEQQQNLTAVVWLYRGEGERFVELVQQYIDK
                     SIFEARSCEQSEALACEPIPDFITQLGKFGQAFQPFMDKLEQGGVSVEPYVDFLNAKG
                     SVEGGWSAFQILTNDLQKWWNEYDYNDAASLLDFIDKDTCLKDLAEQSRNLIKEIDLA
                     YKLATRVIELAEANDAKDSELWDNTLLNGRSRSNLKKTADDARKLAVEQLKQVRYFYK
                     QAHWLLSRFPEGKLCDVEGLVKLVDIKAIEAADWSLTPGRYVGVVPEEVDEDFDFEET
                     LHDIHIELQGLNSEAIELAEKIARNFEELGV"
     gene            complement(5488..5712)
                     /locus_tag="IT904_002486"
     CDS             complement(5488..5712)
                     /locus_tag="IT904_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3192254.1"
                     /translation="MSDPILTIKDVADYLKVNERTIYRLAASSELPGFKVGNSWRFKQ
                     SELEQYIAAQHNRAGVSEAMKSPNSKSEDN"
     gene            complement(5810..6361)
                     /locus_tag="IT904_002487"
     CDS             complement(5810..6361)
                     /locus_tag="IT904_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP3192255.1"
                     /translation="MPQSKILVDTNAYLRLAKTVRPLLFVPFGDNEYCLYILPELNEE
                     LAGRKLQSKFPWVDDEEFAENRKHFPQIGKKQKKSIQQTFEYVWDHVQTELPGPSKVD
                     TWYVAYALELGVPVVTDDQDMTDLAKAFDAQVMPTLELLKIMLDCGHTDMKTINGLVE
                     YWKYFADMPANFKPDYQRLFGDQ"
     gene            complement(6361..7503)
                     /locus_tag="IT904_002488"
     CDS             complement(6361..7503)
                     /locus_tag="IT904_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3192256.1"
                     /translation="MQKRLNTDKAQQAIDKAGLTQTAVADALGVTKEAVSQWLLHKSF
                     PRPNKLLQLGKLLNLSFDDLVIKEDPHAPKVAFRKMRGTKTKDYHIEKAQEIGRFLRH
                     LVPYLPFDTFEMPPVLKSPSCDYDYLRTVTAKVREDINLGPVECVDFTHLIRRFSELQ
                     AVVVPVMWGAKQRHENAVHIHLPDSGSTWVYLNLDTNTHDFKFWMAHELGHCLSPSLE
                     GDDAEDFADAFAASLLYPHELAEQAYEVLVKKPSTAAKINTLKQMAELQVISPNTILQ
                     QVNKYAKRLDRAEIIMGNSFFGAVTKFNQQFLNISEALFGDNELDEYGKPSARDYIEK
                     VEGAFETPFFDMLRQYLKEHDKGPGYVQTVLDMPLLDAQSIHAELT"
     gene            complement(7564..8040)
                     /locus_tag="IT904_002489"
     CDS             complement(7564..8040)
                     /locus_tag="IT904_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAP3192257.1"
                     /translation="MVKRFRRMSDSDINTIVADLDRWALGELGSKLTWAVLEERFGFS
                     RQSLQAKSEIKAAYDNAKRALSGGLVKTKEQATKEAEELQVEVDRLKAELDAYKRKEE
                     QWLRRWQQIAFHVRQKGIQMASVDKAPPEGAELPSNTETAQILRPFDKEVPPSGRV"
     gene            complement(8033..10042)
                     /locus_tag="IT904_002490"
     CDS             complement(8033..10042)
                     /locus_tag="IT904_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP3192258.1"
                     /translation="MSDGRSNRKAKVVPFVDRLERDRNENLNSLVSKAKLMKLEGFES
                     VVWEDPEWQVDAGRLVKLTGKNTKSASFGFLLSPKLGSAPLDGCWELVAKALFVLRFH
                     RKYQSAPNQRSFITAIGYVSFAAKQLGQELVGLTPEALDNASGLISKHYSETTAYNLH
                     KHVAEFAAHCDSNGLCRVLFQYKYAKMKRPASTGGINHKRLDDPEALDTKSDKLVDPA
                     VFRVIGELYLNVPKEHKYRFYILMLTLLACTGRRFSEISLLPNQQLSSDEEGIAYIEY
                     FPRKASRGDVFTPKRRLYLPSEVTPIVGDVLVELAEITFEARLTAEEIHKVGEPDLRF
                     LDGIPEDKKLYAADLTELGISHTVLGTTGWLRKQNLAWPDHSALTKQGIKPANPIFYT
                     YKSGLVEYCFRDFSGAYLSALHTDQFGKEYYLKDLLFIRSLGLSSGSYAHWLATSCSQ
                     SMFTTFLRYFPALAAEYASSSIEVDFTSHHFRHTLNTLLDEGGLSDLLQTEWFGRTNP
                     RDTKAYQHTSREKRALMLREDIKKGLVGGQLAEQIKVVPVEVQDAILKARIQAVHDVG
                     TGICIHNFSQTPCERHLQCSADCKDYVWAKDDKGRLDEQKRQYALTALARKKAEQQLD
                     STKPKKSADWLAHNDKKLKTLAAQLADNGVEHFDPEQYLHEVEHG"
     gene            complement(10035..11633)
                     /locus_tag="IT904_002491"
     CDS             complement(10035..11633)
                     /locus_tag="IT904_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3192259.1"
                     /translation="MNLLLHKAPQISDSALELPSQFFTREGVKVEITGDRWTFPLLYR
                     HSRLDFSRVENDTLKTSIKRCIIDQASRVSTHAGCQYWSDISAAIVSRQKKHSLLPDI
                     SADEFQERLIAVMEEAISQARNKHRLWALYRPIQWYIWCSENYSELGFSIAYAQELDS
                     LEIPGNPKGEAVRQEDPECGPLHRTLELQPIINALKQDQSKVYEHLQQKTAVALSIAF
                     GRNPANLTFLKETDLVDLTPGSSPPCYVLKMPRIKKRQLAPRDDMIDEYLEPEIAKHV
                     LELIDASRHRKLLIEVDGKAVEVTRPLFINPRKNAYAIDSKRWDEAYNMLSTGIANLV
                     KAFIKRHNIISPLTGELLHVTPRRLRYTLATGLAAEGISKRELARILDHTDTQHVNVY
                     FEMAGKIVEHLDKATAKGFSKYLNFFKGRLIDSDEDAVKGERDDKHLTFVDDQNPADQ
                     ADIGVCGESSVCHLNPPYSCYLCPKFQPYRHADHEHVLECLLAGREERLKKYENARLG
                     MQLDEVIAAVAQVAKLCEEGGHHV"
     gene            complement(11630..12805)
                     /locus_tag="IT904_002492"
     CDS             complement(11630..12805)
                     /locus_tag="IT904_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3192260.1"
                     /translation="MRIVTVSHRSGDLLPILLDGDGLPIPSPNEFLLSRRNLSTNTLI
                     RNLRELSVLYRWLEDRQIDLRARLKIGTLFTEAEFSGGLIEFLRKDMESEDKVVSPHT
                     FNNRLATIRQYFVWELDVYISSLPLSDSQYEVLQAARKRLMGWIDRAFINTPRAGSIP
                     RKSLTNDEAEFLLQCLNPESPDSFAHFEPVKFRNFAAVSLMLNCGLRPGELLSLRVED
                     IQIGSISSVTVKRRSPDPLDKRKPRPSVKRNGRVLPLEGHHFVRMLDRYIVEWREILE
                     QKSSSETDYLILSDEGDPLSHSSLTQLFSRLRSEYSGLLPEILTPKSLRHTFSSRMEQ
                     VLRAAGMEEDRRKQALAMLRGDSSLESQSVYIAQEVEEQARRALSDYQKKLITGFNK"
     gene            complement(13023..13559)
                     /gene="ssb1"
                     /locus_tag="IT904_002493"
     CDS             complement(13023..13559)
                     /gene="ssb1"
                     /locus_tag="IT904_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAP3192261.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            13813..16635
                     /gene="uvrA"
                     /locus_tag="IT904_002494"
     CDS             13813..16635
                     /gene="uvrA"
                     /locus_tag="IT904_002494"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP3192262.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(16670..17026)
                     /locus_tag="IT904_002495"
     CDS             complement(16670..17026)
                     /locus_tag="IT904_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP3192263.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(17030..17446)
                     /locus_tag="IT904_002496"
     CDS             complement(17030..17446)
                     /locus_tag="IT904_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP3192264.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(17557..18270)
                     /locus_tag="IT904_002497"
     CDS             complement(17557..18270)
                     /locus_tag="IT904_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAP3192265.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            18672..>18836
                     /locus_tag="IT904_002498"
     CDS             18672..>18836
                     /locus_tag="IT904_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192266.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFFLYIETQLKIAK
                     DFLDLEKKYQV"
BASE COUNT         4687 a         4741 c         4444 g         4964 t
ORIGIN      
        1 taaagagcca tcagctgatg aaaagcggga tacattcatg agtctttcac cgaagatgaa
       61 gccagcgcga tggggtaaac cacgccgctg cctgcttgct ggaggagtgc cgcgacgacc
      121 gttagcgtcc agccggagtc cactatgtca tccactaaca aaaccggttg cccctgataa
      181 agctgagtca cagcaaatgc accatccaga ttgcgacatt ggtggaagcg attctgctgg
      241 cctttttgcg gctgattgtc tttgactttg ctaactgcat ccacaaacgg cagccccaag
      301 cgtgcagcca gccggcgagc aaaatccggt accagttcag gatgattacg cgacggcacg
      361 caacataccc attgaggtgc ggggttaggc tgccaacgct gctgaatcat ttctgccatg
      421 gcttcaacca actcatcact gaaacgcccg gcatgtttgt tgtcggcaac cgtccgcccc
      481 caaccggcat cgccccagcg ggataaaaca cggccctctt gagcgactaa gtcaccgaga
      541 ttacgcggaa attcatattc aacaaatgtt ctcgctgcct catcattcga agctgctatt
      601 tgcttacggg gcttaataat ttcttctgag tgtcttaaaa actgagttgc tcttattcca
      661 atttcttgat cgaaaacttt ggtaagcact ggctccgcaa tacagttctc acatttgcca
      721 caagtttcct gtgaagggtc atcaagcgca ttacgtaaag ccaacattaa acaacctgaa
      781 gtattgagat aatcattaat ctcgctccat tcgcgctcac gaatctctgt aattttacga
      841 atatgctcta gatccatttg gtagcgaaca ggagctctaa accaagtctt tccaatcgca
      901 atgacaggcg atggactctc agtacgcaaa tatttcaaaa catgctctat tcgcttttgc
      961 ggcaggtttg cttctttctc taacttctca agtgacaatc cgtctgcatc ttctaatgct
     1021 gcaagtagac gctgaacatc atcttgactt ggaaaggctg ttcggcgaaa gaaagcatga
     1081 atgtcgttat cctcagtacc tgacattagt acgccaatcg cattttcgat tccacgaccg
     1141 gcgcgaccta cttgttgata gtaagcaact atcgaacttg gggcttggta atgaagcaca
     1201 aagcctaggt caggtttgtc ataccccatc cctaatgcag tagttgcaac aagaactttg
     1261 attttgtttt ctagtaactg agtttccaga aattggcggt agagatctgt atcttctttc
     1321 cctgcataat taacaaaatt aggatgcgtt acgccactgt aatatgccag tgcagatatc
     1381 ccctgctgat tcaaccattt tgcaaccaaa tcggcatcac gctgagtcag tacgtagaca
     1441 atacctgtac ccggcaacgt gggtatcacc tgcgccaacc aagccaaacg agatgcctga
     1501 tccggtaaca ccatggtctg caaggagaga ctttcccgaa tcaacggacc acggtgaatt
     1561 tgaatatttc caagctgagt ttgaatatcc gcaaccacgc ggttattcgc cgtggctgta
     1621 gtgcccagca ccggcgtatt ggcaggcaac tggcgaagaa tattcacaat acgacggtag
     1681 tcggggcgga agtcatgacc ccaatcggaa atacagtgcg cttcatcaat caccatcagg
     1741 gctatgcggt cggcaatggg ctgcaacaca gtttcaataa agctgtcatt agccaagcgc
     1801 tcaggggaaa tcaacaagca gtcgatttga ttattcagaa tccgctgagt taccgaacgc
     1861 caatcatcag tattggttga attcatcgtc tcggcgacta tgccaagtcg agcagcagat
     1921 tcaatttggt tacgcatcaa ggccagcaaa ggcgatacga taatggttgg ccccatacca
     1981 cgatcgcgga aaattttggt actgataaag taaaccgagc ttttacccca accggtgcgc
     2041 tgtaccacca acagtttctg ccgatgattc accagtgcat caatggcctc ccactgtcca
     2101 tcacggaatt cggcggcggg attagctaga gcggtttgta ataattgttg ggcttgggca
     2161 cggttcatga tcactccttg atttacacgg ttagctcgcc gctcataagt ttgggtagta
     2221 gcaggtcacg tgcttgggct agcttctcgt tatattcttt tagtttgtct atctgtttga
     2281 aaatgttttc tacctgaact tcgaattcat tcattaattt cgattcaggg cacagaacat
     2341 cgaccttatg aacgtcattt cgatttaatg tgggaacagc cgcaccgccg ttatagcctt
     2401 caagcttcat tgctcttagc aaatatgttg cgaatatggg tgacgctttt ttgaattcct
     2461 tcacccacaa tgtcgtattg agtggccaat agtctttagc aacatacatt accgttccca
     2521 gtgaaccaga tcttcccgtg acgacaccag ggccttttac cttcgccaca ttatggaatc
     2581 cattaatacc tgtggatgca tagatgggaa cgcttccttc tatacgtttg gaaacaggca
     2641 agtcgaaccc acgctgaaga accagtaagt tctctaaagg ttcctttgac cacccttccg
     2701 gcacaccatc agtaatctta acttgcttat gtccggggaa gcgcaagtga acaaaccatt
     2761 cctgatacag caggcgagcg gattcttcca gcaactgaat acggcggcgg ttgttttcga
     2821 tgaggtcgtt ataagtctta atcttttgga cgattttatc ttgaacatca acagggggga
     2881 gtggtatttc aaatccctct agcatgcctt ttgtcagtgc ttttctggtt ccaccagtac
     2941 cagcccaaga aagcatcttg gcctgcatga agggtgatac catgtagaaa gcaagaaaat
     3001 gtggcagaag cttctttgga tttgttctta aaatcgcaac gtgctgattt acccttgctg
     3061 ggagtactgc atcatggacc aaacagcatc gtgcaacaga atcgccggta atgttgacaa
     3121 gaacatcctc cttctcaaca ctaacgcctt tcattttttc tgctgttttt tcatcgatat
     3181 aaactaagcc atccgttgaa aattcattgt tatatacatt ttggctacgg attaacgttg
     3241 taccttcaga tatatagaca gaatcaccac ctcttggagt tgagccgcta ccaattttga
     3301 gggttaacgc tgacagaggt tcgctttgcc agctcatacc cccaactcct caaagttacg
     3361 ggcaattttt tcggctagct ctatggcctc agaattcaaa ccttgcagtt caatatgaat
     3421 atcatgcagg gtttcttcaa aatcgaagtc ttcatccacc tcttccggca ccacacccac
     3481 atagcggccc ggcgtcaggc tccagtcagc ggcttcgatc gctttgatat ctaccagttt
     3541 gaccaggcct tccacatcac agagcttgcc ttccgggaag cggcttaaca gccagtgggc
     3601 ttgcttgtag aagtagcgta cctgcttgag ttgctctacg gccagcttgc gggcgtcatc
     3661 ggcggttttc tttaagttgc tgcgactgcg gccatttaac agcgtgttat cccagagttc
     3721 gctgtctttt gcatcattgg cttcggccag ttcaatcacg cgggttgcca gcttataagc
     3781 caggtcgatt tctttaatca ggttgcggct ttgttcggcc aggtctttta ggcaggtatc
     3841 tttatcgatg aagtccagca ggctggcagc atcgttatag tcgtattcgt tccaccattt
     3901 ctgcaggtcg ttggtcagga tttgaaacgc ggaccaaccg ccttcgaccg agcctttggc
     3961 attcagaaaa tcgacataag gctcaacact cacgccgccc tgctccagtt tgtccataaa
     4021 gggttgaaag gcttggccga attttcccaa ctgagtgata aagtctggta ttggctcgca
     4081 ggctagcgct tcagattgtt cacaggagcg agcttcgaaa attgacttgt cgatatactg
     4141 ctgcaccagc tcaacaaaac gctctccttc gccacggtat agccagacca cggcagtcag
     4201 gttctgttgc tgctcggggc tgaagtcgta aatcttgcgg gtaactttgc ggtagacgtt
     4261 gcgtgcatcc agcattaaca ccttgtcttt caggtgcgcg ggcttgtttt tattcagaaa
     4321 ccacaactgg cagggcacgg tgcgggtata gaaaaagtta ccgcgaatat cgaccatgat
     4381 gtccacatga ccggttttga ccaactgctc gcgcacctta gcttcgtccc gcccagcact
     4441 ggaagcctgg gatgacatga caaaacctgc tcggcccgaa tcgttgagat agctataaaa
     4501 gtactgaatc cacaggtagt tggcgttact gacttttttg tttttattta cgcccggcaa
     4561 gccaaacggc aagcggcgct tatcgccttt gattttatcg gcgtcgactt catccacgtt
     4621 aaagggcggg ttggccatca catagtcgac agtgccgaac aggccttcgt ggggatcgtt
     4681 gtagtaggta atggcggctt caccaccttc cacgttgcct tcaaggccgt gtaccgccag
     4741 gttcattttg gccaggcgtg tggtcacgcg atttttttcg tggccgtaaa aggtgagttc
     4801 atgcgggtct tgcgcgtgcc gctccataaa gtgggcactt tgtacgaaca tgcccccgga
     4861 accgcaagcc gggtcaaaaa ttttgccgtg atctggctcc aacacattca caatcaactg
     4921 cactaacgat accggcgtga aaaattcacc accatcgtgc gcaccctgat cggcaaactg
     4981 ggtgaggaag tattcataga tacggccaaa gatatcaccc gtggcttttt taagctcttc
     5041 tgggttgagc gtacgcagca acatacctaa tacatcattg ggaatgttgt tgtattcctg
     5101 cttcggcagc tggttacgga ggttggtgta atcggtctcg atggagtcca tggcatgaat
     5161 aatggcttcg gcgcgattat cagcatccgt cagagcaatg aggtagtcga actgtgcttc
     5221 tggcttgaga tagatggcgc tctttttaga gaaatcttct tttgtaagct cgcgggtttt
     5281 accgccacgg ctgggcaggt ttgcgattat ctcgtctttt agcgataagt agcggctgta
     5341 agcatgtcgc aggaagatca gccccatcac cggcaaaaag tattcgttac tggccagctc
     5401 ggagttggcg cgtagggtat cagccgattt ccacaggcgt ttttcaattg cttcgatgtt
     5461 ttccagttgt gccatggggc cggattccta gttatcttct gatttactgt ttggtgattt
     5521 cattgcttcg ctgacaccgg cgcggttgtg ctgggcggcg atgtattgct ccagctcgct
     5581 ctgtttgaat cgccaggagt tacccacttt aaagcccggt aattcgctgc ttgcagccaa
     5641 ccggtatatg gtccgttcat tcaccttgag gtaatcggcc acgtctttga tggtcaatat
     5701 ggggtcactc atgacgaggt atctcagcta tgtcggaggt gtcgaaaaag tacaagactg
     5761 tcaatatata ggatctggga gggagatcaa gtaagaaaag ctttcaggct tattgatcac
     5821 caaacaggcg ctggtagtcg ggcttgaagt tggcaggcat gtctgcaaag tacttccagt
     5881 actcaaccag gccattgatg gtcttcatgt ctgtatggcc acagtccagc atgattttga
     5941 gcagctctaa ggttggcatg acctgcgcat cgaaggcttt ggccaaatca gtcatatcct
     6001 gatcgtccgt caccaccggt acacccaact ccaatgcata ggcgacatac caagtatcca
     6061 ccttggacgg acccggcaac tcggtttgca catgatccca cacgtattcg aaggtttgct
     6121 gaatggactt cttttgtttt ttgccgattt gggggaagtg tttgcgattc tcggcgaatt
     6181 cctcgtcatc tacccaaggg aacttacttt gcagcttcct acccgccagc tcttcattga
     6241 gttcaggcag aatgtacagg caatactcgt tgtcaccaaa gggcacaaac agtaaggggc
     6301 gtacggtttt ggccaacctt aggtaggcgt tggtatcaac gaggattttc gattgcggca
     6361 tcaggtcaac tctgcgtgga tactctgtgc atcgaggagt ggcatatcca gcacagtttg
     6421 aacataaccc ggccctttat cgtgctcttt cagatattgt cgaagcatat cgaagaaagg
     6481 agtttcgaaa gcgccttcta ctttttcgat gtagtcgcgc gcgcttggct tgccgtattc
     6541 gtccagctca ttatcaccga acagggcttc gctgatattt aggaattgct ggttaaattt
     6601 ggttaccgca ccaaagaagg aattacccat tataatttca gctctatcaa gccgctttgc
     6661 gtacttgtta acttgctgaa ggatagtatt tggggaaatt acctgcagct ctgccatctg
     6721 ctttaacgta ttgatctttg cagcagtcga cggcttttta accaacactt cgtaggcctg
     6781 ctccgctaat tcatgagggt aaagcaagct cgcggcaaaa gcatcggcga agtcttccgc
     6841 gtcatcaccc tcaaggcttg gcgaaagaca gtgccccaac tcatgtgcca tccagaactt
     6901 gaagtcatgg gtattggtat ccagattcag gtatacccat gtgctgcccg agtcaggaag
     6961 gtgtatgtgt actgcatttt catgacgctg ctttgctccc cacataactg ggaccacaac
     7021 ggcctgtagt tcgctgaagc ggcgaatcaa atgcgtgaaa tccacacact ccaccgggcc
     7081 aagattaata tcttctctga ctttcgctgt taccgttcga agatagtcat aatcacagct
     7141 cggcgacttg agtaccggcg gcatttcaaa ggtgtcaaac ggcaaatagg gcacgaggtg
     7201 acgcaaaaag cgaccaatct cttgcgcttt ttcgatatgg taatccttgg ttttggtacc
     7261 gcgcattttg cggaatgcca ctttcggggc gtgcggatct tccttgatga ccaaatcatc
     7321 aaaagagaga tttaacaact tgcctaactg aagtagcttg ttaggtcggg gaaaagactt
     7381 gtgcaaaagc cactgggaaa cggcttcttt ggttacgcct agagcatccg cgacggcagt
     7441 ttgggttaac cctgccttat cgatcgcctg ctgtgcttta tctgtattta gtcttttttg
     7501 catgcgcgaa gtatatgccg cagtcaagtt ttgtcaagtt taaaatctcc agactagtgc
     7561 ctactaaacc ctccctgaag gtggaacttc cttatcaaac ggccggagta tctgcgcggt
     7621 ttcagtgttt gagggcagct cagccccttc tggtggggcc ttatcaacgc tagccatctg
     7681 tatccctttc tgccgcacat gaaaggcgat ttgctgccag cggcgcagcc attgctcttc
     7741 ttttcgcttg taggcatcta gctccgcttt gaggcgatct acctctacct gaagctcttc
     7801 ggcctcttta gtggcctgct ctttggtttt caccaatccg cccgacaatg ctcgcttggc
     7861 gttgtcatag gcggccttga tctcagattt agcttggagc gactggcgac taaagccaaa
     7921 gcgctcttct agcacagccc aggtgagttt tgatccaagc tcacccaacg cccagcgatc
     7981 caaatcggca acgattgtat tgatgtcact gtcggacatg cgtctgaagc gcttaaccat
     8041 gctctacctc gtgtaaatat tgttcgggat caaagtgctc caccccgttg tcggccagtt
     8101 gggcagccag tgttttcagt tttttatcgt tgtgtgccag ccagtcagca ctcttttttg
     8161 gcttagtgct atccaactgc tgttcagctt tttttcgggc tagagcagta agcgcatatt
     8221 ggcgtttttg ctcgtccaga cgacccttat catccttagc ccacacgtag tctttacagt
     8281 ccgccgaaca ctgcaaatgg cgctcgcagg gggtttgaga gaaattgtgg atgcagatac
     8341 ctgtaccgac atcatgtacg gcctgaattc gtgctttcag aatggcatcc tgtacttcta
     8401 ccggaaccac tttgatttgc tcagctaact gacctccaac caaacctttt ttgatgtctt
     8461 cacggagcat cagtgctcgt ttttcacggg atgtatgctg ataggctttg gtgtctcttg
     8521 ggttcgttcg accaaaccat tcagtttgca gcaggtcact taacccgccc tcatcaagca
     8581 gagtattgag cgtatggcgg aagtgatgac tggtgaagtc cacctcgata ctgctggagg
     8641 cgtattcggc ggcgagcgca gggaaatagc ggagaaatgt agtaaacatg gactggctac
     8701 aacttgtggc cagccaatga gcgtagcttc cgctggaaag tccaagagaa cgaatgaata
     8761 aaagatcttt taggtagtat tctttaccaa attggtccgt atgaagagcc gataaatatg
     8821 ctcccgagaa atcacggaaa cagtattcaa ccaatccact tttgtaagtg taaaaaatgg
     8881 gatttgcagg cttaattccc tgcttggtta atgcgctatg atctggccac gcgaggtttt
     8941 gcttcctgag ccagccagtt gtgcccaaaa cagtatgact gatccccaat tcagttaaat
     9001 cagccgcgta gagcttttta tcttcaggta tgccatccag aaaccttaga tcaggctcac
     9061 caactttgtg aatttcttcc gctgttaaac gcgcttcaaa tgtaatttcc gctaactcaa
     9121 cgagcacatc accaacgatg ggggtcactt ctgacggcaa gtaaagtcta cgcttaggtg
     9181 tgaaaacgtc accacgactt gccttccgcg gaaagtattc gatataggca atcccctctt
     9241 catcagaaga caattgctga tttggtaata gtgaaatctc actgaatcgc cttcctgtgc
     9301 aagcaagtag cgtcaacatc agaatataga agcgatactt atgctctttg ggaacgttta
     9361 agtagagttc gccaatgact cgaaacacag cgggatcgac aagtttgtcc gacttcgtat
     9421 ccagagcctc tggatcatcc aaccttttgt gatttatccc tcccgtgctc gcgggacgct
     9481 tcattttggc gtatttgtac tgaaataaaa cccgacatag accattcgaa tcacaatgcg
     9541 ctgcgaactc tgcaacatgc ttgtgaaggt tgtaggcagt ggtttcactg tagtgttttg
     9601 aaattaaacc agacgcatta tccagagcct ctggcgtaag cccaactaat tcctgcccta
     9661 actgtttagc tgcaaatgag acatatccaa tagcggtaat gaaactgcgc tgattcggtg
     9721 ctgattgata ttttctgtgg aacctgagaa caaacaatgc tttggcaacc agctcccagc
     9781 aaccgtctag tggcgctgaa cccagcttcg gagatagcaa aaaaccaaaa gaggctgatt
     9841 tcgtattttt accagttaac ttgactaggc gacctgcatc gacttgccac tcggggtcct
     9901 cccaaacaac cgactcaaac ccttccagct tcattagctt tgctttgctg actaaagagt
     9961 tgagattttc attcctgtca cgctcaagac gatcaacgaa agggacgacc ttggccttgc
    10021 gattagagcg accatcagac atgatgcccc ccttcctcac agagtttggc tacttgtgcg
    10081 accgctgcga ttacctcatc gagctgcatg cccaatcgcg cattctcgta cttcttcaaa
    10141 cgctcttcgc gaccggccag caggcactcc aagacatgct catgatcagc atgacgatat
    10201 ggctgaaact tggggcacaa ataacatgag taggggggat taaggtggca gacgctagat
    10261 tcaccacaca cgccaatatc agcttgatca gcaggattct gatcatctac aaacgttaga
    10321 tgcttgtcat ctcgctcacc tttgacagca tcttcatcac tatcgattag cctgcctttg
    10381 aaaaaattga ggtacttcga gaaacctttc gcggttgctt tgtctaggtg ttcaactatt
    10441 tttccagcca tctcaaaata gacattcacg tgctgggtat ctgtatggtc cagtattctg
    10501 gccagctcac gcttactgat tccctcagca gccagcccag ttgccaaggt atatcgcagc
    10561 cgccttggcg tgacatgcaa taattcgcca gtaagcggcg agataatgtt gtgacgtttt
    10621 ataaaagctt tcacaaggtt cgctatccca gtggacagca tgttataagc ctcatcccaa
    10681 cgctttgaat caatcgcata agcatttttt ctggggttta taaatagagg tctggtgact
    10741 tctactgcct ttccatctac ctcaattaga agcttcctat gcctggacgc atctataagc
    10801 tccaaaacgt gctttgcgat ctcaggctct aaatactcat cgatcatgtc atcccttgga
    10861 gccaactgcc ttttctttat tcttggcatt ttcaagacat aacacggtgg cgatgaaccc
    10921 ggtgtcaggt caactaaatc agtttctttt aaaaatgtta aattcgcggg atttcggcca
    10981 aaagctatag atagagccac agctgttttt tgctgcaaat gctcgtagac tttactttga
    11041 tcctgcttaa gcgcattgat tataggctga agctccagcg tcctatgcaa cggcccacat
    11101 tctggatctt cctgacgaac cgcctcaccc ttagggttgc ctggaatttc tagagaatcg
    11161 agctcttgtg cataggctat cgagaacccg agctccgaat agttttcaga gcaccagatg
    11221 taccactgaa ttggccggta caacgcccac agcctgtgct tatttcgcgc ctgactgatt
    11281 gcctcttcca ttacagcaat cagacgttct tgaaattcat cagcggaaat gtcgggcaac
    11341 aaggagtgct ttttttggcg agagactatt gcagcggata tatcactcca gtactggcac
    11401 cctgcatgag ttgacaccct tgatgcctga tcaataatac agcgcttaat cgaagtttta
    11461 agcgtgtcgt tttcaactcg agaaaaatca agcctgctat gccgatacag aagaggaaat
    11521 gtccatctat caccagtaat ctcaactttt acaccctctc gagtaaaaaa ttgagagggt
    11581 aactctagcg cagagtcaga aatctggggc gctttatgca agagcaaatt catttattga
    11641 accctgtaat caatttcttt tgatagtcag aaagtgcccg acgagcctgc tcttcaacct
    11701 cttgagcgat atacacagat tgggactcca aacttgagtc tcctcgcaac atagccaagg
    11761 cttgcttcct tctatcctct tccatgcctg ccgccctaag tacctgttcc atacggctag
    11821 aaaaggtatg cctcagagat ttaggtgtaa gaatctccgg aagcaaacca gaatattcac
    11881 tccgtagacg agaaaaaagt tgcgtaagag acgaatgaga cagagggtcg ccttcatcac
    11941 ttaagatcaa atagtcggtt tcactgctcg atttctgctc caaaatctcc ctccactcaa
    12001 ctatgtagcg atccagcatt cgcacaaagt gatgtccttc taaaggcaat accctgccat
    12061 tgcgcttgac actgggtctt ggttttctct tgtcgagcgg gtccggggac cttcttttaa
    12121 cagtcaccga cgagatcgag ccaatctgaa tatcctctac acgcaaacta agcaattccc
    12181 cagggcgaag tccgcaatta agcatcagag acaccgccgc gaagtttcta aatttaacag
    12241 gctcgaaatg cgcaaacgaa tctggacttt caggattcag acactggagt aagaactcgg
    12301 cttcgtcatt ggttagagac ttcctcggaa tacttccagc ccttggagta tttataaagg
    12361 ctcgatcaat ccatcccatt agacgcttcc ttgcagcctg aagaacctca tattgggaat
    12421 cacttagtgg taaagatgaa atataaacat cgagctccca tacgaagtac tgtcggatcg
    12481 ttgctaatcg attattgaat gtatgaggcg agacaacctt atcttcagat tccatatctt
    12541 ttcttaggaa ctctatcaga ccaccactga attcagcctc tgtgaatagt gtgccaattt
    12601 tcagccttgc tctcagatcg atttgtctgt cctccagcca tcgatacaaa acagacaact
    12661 ctcgaagatt cctgattagc gtgtttgtac ttaagtttct tcgactcaac agaaattcat
    12721 ttggagaggg aatcggcaaa ccatcaccat ccaaaagtat tggcaataaa tcacctgatc
    12781 tatgacttac agttacgatt ctcattcaag ccatatcttt acaattacaa aacttgaaaa
    12841 cctacaggtt tacaaaaata ataatatacg aattctaata atcacacatt ttttaggcaa
    12901 taaaaagccc tctagtatct ttacagacaa aagagggcta tttattaact taacctatta
    12961 atttatatgt cttttttaaa acaaaccaaa cctaaaagat tacaaattaa tatttatcag
    13021 ggctagaacg gaatgtcatc atcaaagtcc atcggcggct cgttagacgg cgctgccgga
    13081 gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact gattgccacc ctgcggctgc
    13141 tgaggctgac cccaaccgcc ctgcggctga ccaccaccga tattaccacc tgccggagcg
    13201 ccaccaccct gacgaccacc cagcatctgc atggtgccgc caacgttcac cacgacttct
    13261 gtggtgtagc gatcctgacc ggattgatcg gtccatttac gggtacgcag ctgaccttcg
    13321 atataaacct gagaaccttt acgcagatat tcgctggcca cttctgccag tttgccgaac
    13381 agcacaacgc ggtgccattc agtctgttct ttcatctcgc cggtcgcttt atcacgccag
    13441 gattcggaag tagccagcgt aatgttggca actgcgccac catttggcat gtagcgtact
    13501 tccgggtcct gacccagatt accaacgaga ataaccttgt ttacgcctct gctggccatg
    13561 ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa cgcgcgattg taccattacc
    13621 aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa caaacctggc cagacattgt
    13681 tacacaacac tccgggtaat gcattccaat actgtatatt cattcaggtc aatttgtgtc
    13741 ataattaacc gtttgtgatc gccggtagca ccatgccacc gggcaaaaaa gcgtttaatc
    13801 cgggaaaggt gaatggataa gatcgaagtt cggggcgccc gcacccataa tctcaaaaac
    13861 atcaacctcg ttatcccccg cgacaagctc attgtcgtga ccgggctttc gggttctggc
    13921 aaatcctcgc tcgctttcga caccttatat gccgaagggc agcgccgtta cgttgaatcc
    13981 ctttccgcct acgcgcggca gtttctgtca ctgatggaaa agccggacgt cgatcatatt
    14041 gaggggcttt ctcctgccat ctcaattgag cagaaatcga cgtctcataa cccgcgttct
    14101 acggtgggga caatcaccga aatccacgac tatttgcgtt tgttgttcgc ccgcgtcggc
    14161 gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc aaaccgtcag ccagatggtg
    14221 gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc tgctcgcgcc aatcattaaa
    14281 gagcgcaaag gcgaacacac caaaacgctg gagaacctgg caagccaggg ttacatccgt
    14341 gctcgtattg atggcgaagt ctgcgatctt tccgatccgc cgaaactgga actgcaaaag
    14401 aaacatacca ttgaagtggt ggttgatcgc ttcaaggtgc gtgacgatct tacccaacgt
    14461 ctggccgagt cgtttgaaac cgcgctagag ctttccggtg gtaccgcggt agtggcggat
    14521 atggacgacc cgaaagcgga agagctgctg ttctccgcca acttcgcctg cccaatttgc
    14581 ggttacagta tgcgcgagct ggagccgcga ctgttttcgt ttaacaaccc ggcgggggcc
    14641 tgcccgacct gcgacggcct tggcgtacag caatatttcg atcctgaccg cgtgatccag
    14701 aatccggaac tgtcgctggc aggtggtgcg atccgtggct gggatcgccg caacttctat
    14761 tatttccaga tgctgaaatc actggcagat cactataagt tcgacgtcga agcgccgtgg
    14821 ggcagcttga gcgcgaacgt gcataaagtg gtgttgtacg gttctggcaa agaaaacatt
    14881 gaattcaaat acatgaacga tcgtggcgat acctccatcc gtcgtcatcc gttcgaaggc
    14941 gtgctgcaca atatggagcg ccgttataaa gagacagaat ccagtgcggt acgtgaagaa
    15001 ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg aaggaacgcg tctgcgtcgg
    15061 gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcccg ccatctccga catgagcatc
    15121 ggtcatgcga tggaattctt caacaatctc aaactcgcag gtcagcgggc gaagattgcg
    15181 gaaaaaattc ttaaagagat cggcgatcgt ttgaaattcc tcgttaacgt cggcctgaat
    15241 tacctgacgc tttcccgctc ggcagaaacg ctttccggcg gtgaagccca gcgtatccgt
    15301 ctggcgagcc agattggtgc gggcctggtt ggcgttatgt acgtgctgga cgagccatct
    15361 atcggcctgc accagcgcga taacgagcgc ctgttgggta cgcttatcca tctgcgcgat
    15421 ctcggtaata ccgtgattgt ggtggagcac gacgaagacg cgattcgcgc cgctgaccat
    15481 gtgatcgaca ttggcccggg cgcaggtgtt cacggcggtg aagtggtcgc ggaaggtcca
    15541 ctggaagcaa ttatggcggt gccggagtcg ttaaccggac agtacatgag cggtaaacgc
    15601 aagattgaag tgccgaagaa acgcgttccg gcgaatccgg aaaaagtgct gaagctgaca
    15661 ggcgcacgcg gtaataacct gaaagacgtg acgctgacgc tgccagtcgg tctgtttacc
    15721 tgcatcacag gggtttcagg ttccggtaaa tcgacgctga ttaacgacac actgttcccg
    15781 attgcccaac gccagttgaa tggggcgacc atcgccgaac cggcaccgta tcgcgatatt
    15841 cagggactgg agcatttcga caaagtgatc gatatcgacc aaagcccaat tggtcgtact
    15901 ccgcgttcta acccggcgac ctataccggc gtgtttacgc ctgtgcgcga actgtttgcg
    15961 ggcgtaccgg aatcccgtgc gcgcggctat acgccgggac gtttcagctt taacgttcgt
    16021 ggcggacgct gcgaggcctg tcagggcgat ggcgtgatca aagtggagat gcacttcctg
    16081 ccggatatct acgtgccgtg cgaccagtgc aaaggtaaac gctataaccg tgaaacgctg
    16141 gagattaagt acaaaggcaa aaccatccac gaagtgctgg atatgaccat cgaagaggcg
    16201 cgtgagttct ttgatgccgt acctgcactg gcgcgtaagc tgcaaacgtt gatggacgtt
    16261 ggcctgacgt acattcgact ggggcagtcc gcaaccaccc tttcaggcgg tgaagcccag
    16321 cgcgtgaagc tggcgcgtga actgtcaaaa cgcggcaccg ggcagacgct gtatattctc
    16381 gacgagccga ccaccggtct gcacttcgcc gatattcagc aactgctcga cgtactgcat
    16441 aaactgcgcg atcagggcaa caccattgtg gtgattgagc acaatctcga cgtgatcaaa
    16501 accgctgact ggattgtcga cctgggacca gaaggcggca gtggtggcgg cgagatcctc
    16561 gtctccggta cgccagaaac cgtcgcggag tgcgaagcat cacacacggc acgcttcctt
    16621 aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc cttttccttt cagagttgca
    16681 ccagcaattt acgtttttct tccggcagta aattcaccgc ctgctgataa gacgcatcca
    16741 ccagataata gatttgcgaa tctggcagcg aaccgtcgag atacacggtg ctccagtgcg
    16801 ctttattcag atggcggctt ggacgcacat cgctgtgctg ctgacgtagc agctccgcca
    16861 gctccgggct ggttttcagc gaaacagctg ggcgattttc tacttctttc accatcgcaa
    16921 acagtacatc ttccactttg atctgcgtcg ctttccagtc gttatgcacg ctctgttctg
    16981 cgcctggttt tgccatgcaa tattgtagca actccgaaat ggtcattttt tactcccctt
    17041 gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg atgttccccc agccaaatgc
    17101 cttgccaggt gccggtctga atacgccctt tatgcaccgg caatacaagc gatgttccca
    17161 gcattgagga tttgatatga gaaggcatat cgtctgctcc ctcatagtca tgctcataat
    17221 ttccgttgtc gggaacggtg cggaggaaaa aacgctccat gtcgtggcgt acggtgggat
    17281 cgcagttctc attaagtgtc agagaggcgg aggtatgttg cagcaacaga tgcagtaagc
    17341 cgatgttaac gcgcggcata tcagccagct gattcagaat ttcatccgtt accagatgaa
    17401 acccacgaga tttggcgcta agcgtgagcg tcttttgata ccacatgtgc tgctccttga
    17461 taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg ataaaggtgt gattaaaaaa
    17521 acagcattcg ggagagcatc acgctctccc gctctgtcag tattctgaat tgacgatcac
    17581 ctcttcacca aacgcacccg cttgtggcaa gggtttgtag gtagagttgg aggcgcgcag
    17641 aatgcggata ccacgagcgc cgacatcgcg tgcggcggta atatcattat cagaatcgcc
    17701 ataaaaaatt cggatatttt tatcctgcag ccattgcgat tttgtatttt gccctggttt
    17761 atcgcccgca aagatcaccg gattcatgtt ggtggcagga atatgaaaat tatccgccag
    17821 cgtttttgaa accgtttctg ttttcgtcgg gctacgacca gtcacaaaga agatcgcgtc
    17881 accgcggcgt acatgcatat caatcagctg gcgagcgacc tcttttggaa tgctgaattc
    17941 atcccagcca ttgttcattt tttcccagaa cacaggattt ttcagataat cttcgctttc
    18001 tggcgagaag gtttttttgc cgcgccagaa gcccggactg gaaaaaagta ccgtgtcatc
    18061 gatatcaaac cccaccgcca ttggcggacg ccctgcgagg ctattttcaa tttgtgcgac
    18121 cgaaacccaa tgaatgggtg cctgttcagc aagcctggca acgttagtcc cagggttaag
    18181 cggtgaagga gatgaggcca gggcaacagc ggaactgttt agagcgaaca ataagcaaac
    18241 ggcactgatt gcctgtgtga tcttgcgcat atttttccct aaatagtcag tatgttgaaa
    18301 ctttttattg tgagatttgt tgcaaaaacc atctgaccat aacgccagct gccggggaaa
    18361 ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg attatattta acccagtaaa
    18421 tacgttcctg tctttttttg tataaaaaat gtttattact cgatgtaagg atacattaat
    18481 tttacgttta ttatgatgaa tcctcggcaa atgagcgaaa tttgatagaa catttatctg
    18541 ctgtacgatt taatgaactt aatgatacag cattacaaaa acaatcgaag tttataaaga
    18601 tgatttctga ttgaccagcc ccctacccta caatggtgat taattttatt actcgcgatg
    18661 gagatgatga tatgaatata tcatatgtga atagcaacaa aacgacatct ttacctgttg
    18721 agctagatgc actaaataat aaagatatct cttatgccaa agatttcttt ttatacatag
    18781 aaacacagtt aaaaattgca aaagattttt tagacctgga gaagaagtat caagtt
//
LOCUS       DACAPB010000080        18762 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.080, whole genome shotgun sequence.
ACCESSION   DACAPB010000080 DACAPB010000000
VERSION     DACAPB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.080"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..198)
                     /gene="fixB"
                     /locus_tag="IT904_002499"
     CDS             complement(<1..198)
                     /gene="fixB"
                     /locus_tag="IT904_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="involved in electron transfer during carnitine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAP3192267.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTEN"
     gene            complement(218..982)
                     /locus_tag="IT904_002500"
     CDS             complement(218..982)
                     /locus_tag="IT904_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAP3192268.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADTKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            1298..2209
                     /locus_tag="IT904_002501"
     CDS             1298..2209
                     /locus_tag="IT904_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP3192269.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYCSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(2252..3403)
                     /locus_tag="IT904_002502"
     CDS             complement(2252..3403)
                     /locus_tag="IT904_002502"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP3192270.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(3417..5012)
                     /locus_tag="IT904_002503"
     CDS             complement(3417..5012)
                     /locus_tag="IT904_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAP3192271.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLLKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHPPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(5157..5915)
                     /gene="aroD"
                     /locus_tag="IT904_002504"
     CDS             complement(5157..5915)
                     /gene="aroD"
                     /locus_tag="IT904_002504"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP3192272.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVTDLRTVLTILHQA"
     gene            complement(5946..6812)
                     /gene="ydiB"
                     /locus_tag="IT904_002505"
     CDS             complement(5946..6812)
                     /gene="ydiB"
                     /locus_tag="IT904_002505"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAP3192273.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(6824..8089)
                     /locus_tag="IT904_002506"
     CDS             complement(6824..8089)
                     /locus_tag="IT904_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192274.1"
                     /translation="MSQNKAFSTPFILAVLCIYFCYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVTNELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRD"
     gene            complement(8316..9497)
                     /locus_tag="IT904_002507"
     CDS             complement(8316..9497)
                     /locus_tag="IT904_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192275.1"
                     /translation="MYFNYLVHGMGVILMSLNMASLETLWQTNAAGVSIVISSLGIGR
                     LSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLD
                     AGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIM
                     FINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQ
                     WLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFI
                     ALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYSAGSI
                     ATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKENVAPG
                     G"
     gene            complement(9630..9986)
                     /locus_tag="IT904_002508"
     CDS             complement(9630..9986)
                     /locus_tag="IT904_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAP3192276.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(10122..10229)
                     /gene="rprA"
                     /locus_tag="IT904_002509"
     ncRNA           complement(10122..10229)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="IT904_002509"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(10415..11527)
                     /gene="ydiK"
                     /locus_tag="IT904_002510"
     CDS             complement(10415..11527)
                     /gene="ydiK"
                     /locus_tag="IT904_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAP3192277.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            11916..14972
                     /locus_tag="IT904_002511"
     CDS             11916..14972
                     /locus_tag="IT904_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP3192278.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14969..15379
                     /gene="menI"
                     /locus_tag="IT904_002512"
     CDS             14969..15379
                     /gene="menI"
                     /locus_tag="IT904_002512"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAP3192279.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            15479..15667
                     /locus_tag="IT904_002513"
     CDS             15479..15667
                     /locus_tag="IT904_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192280.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            16215..16583
                     /gene="sufA"
                     /locus_tag="IT904_002514"
     CDS             16215..16583
                     /gene="sufA"
                     /locus_tag="IT904_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAP3192281.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            16592..18079
                     /gene="sufB"
                     /locus_tag="IT904_002515"
     CDS             16592..18079
                     /gene="sufB"
                     /locus_tag="IT904_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP3192282.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            18089..>18762
                     /gene="sufC"
                     /locus_tag="IT904_002516"
     CDS             18089..>18762
                     /gene="sufC"
                     /locus_tag="IT904_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP3192283.1"
                     /translation="MLSIKDLQVSVEDKAILRGLSLDVHPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIV"
BASE COUNT         4912 a         4524 c         4629 g         4697 t
ORIGIN      
        1 attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt ttggaccata
       61 aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct gttggcccca
      121 ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa agacccagac
      181 gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct ttcttcagat
      241 gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg tgcttacgtt
      301 ctgtttgcgg cggtacgcga atgccggtca gttcagcaag tggtgcgctc tggctccagc
      361 caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga atggctttca
      421 tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct ggaacagaga
      481 gttcaataac ttcaacgtca tcttcaagcg tgcgttcaat caccagtgta ttgccctgac
      541 gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg accagcaagc
      601 caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa tcgaagccga
      661 tcttttcaac tgctgccgcc agcgcttttg cggtatcgag aggcagtgca tgctcaagtt
      721 gtgcatcctg caccatatac aggctgtgcg gcccgcggga tagcacgtct ttgcgcactt
      781 tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg tcatctgttg
      841 caagctggct tgcagcttca atggcattga gatcgaactg gctgattttg gtgtcggcat
      901 tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca ggcaccagct
      961 taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac catctccgat
     1021 ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt cgacggagca
     1081 agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tcgctaaaac aaaagtgcaa
     1141 tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta aataatcttg
     1201 tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt atccggcata
     1261 tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct gttttgataa
     1321 tgccagtgaa acgctgtttg tcgctggtaa aacgccacgg ctttcacgtt ttgcatttag
     1381 cgatgatcca aaatgggagt ctggacatca cgttcatgac aatgaaaccg agctgattta
     1441 cgtcaagaaa ggggtggcaa ggtttaccat cgattcttcg ttatatgtcg cgcatgcaga
     1501 tgatatagtg gtgatagaac gcggcaggct gcatgcggtg gcctctgacg ttaacgatcc
     1561 ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg aggaaaatca
     1621 tctactgcaa ccgcattctt gtccggtaat tgccgcgggg cagggaaaag aagtcattaa
     1681 aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc aaacatcttc
     1741 gttatgggat gcatttgcct atacattagc aattctttac tacgaaaact ttaaaaatgc
     1801 ttattgttcg gagcaggggt atattaaaaa agatgttctg ataaaagata ttcttttcta
     1861 tctgaataat aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa aatttcgtgc
     1921 cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc caattaacta
     1981 tgttattcaa cggcgtatga cggaagcgaa atggttactc actaatactg aattatcaca
     2041 ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttggca aactgttttt
     2101 gcgccatgtc ggctgttcac ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga
     2161 acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt gttggataaa tctggcggcg
     2221 cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgatagtct ttcaggatct
     2281 gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga
     2341 catcacgcca gaagcgggag acgcgcgctt catcggtata gcccagaccg cccatgattt
     2401 gaatcgcatc atcaatgact tccattgcgg tacgtgcgca atacagtttt gccagcgccg
     2461 cgctggtgcg cagtgactga tgctgatcgg cttgccatgc cactttcagc accatgttgc
     2521 gcatgttgtc aatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac
     2581 caatgggctt accaaaagcg atacgttggt tggcatagcg ggcggcatct tcaaaggcgc
     2641 attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca
     2701 tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacgt
     2761 tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc agcgggttaa
     2821 tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt ttgggatctt
     2881 tcggttgcgg atcgcgcgcc aacaccagca tatacgggta ctctttcgca ccggtaataa
     2941 aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg gtggtggcac
     3001 tgttgttatc tgagccagca cctggttccg tcaacgccag ggcataggcg gggtcgccgg
     3061 tttccagggt gctttctgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac
     3121 tgtgaataca ttgaccgttg gtaattaaaa atgccggagc accgcatttt gacacttcca
     3181 tcagcgccag catttgggtg acgtaatccg cagggatacc accaaattct tccggcacgc
     3241 caagcatgga aataccgtta tccgccagcg cccgcataaa ctcacgcgga tatgtcccgt
     3301 tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac
     3361 tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcattata ctcctgttaa
     3421 tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc
     3481 agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat atctttttgt
     3541 aaatcgacgc caggggcgat ttcgattaaa tgcaggccgt cttctttcag cgtgaatact
     3601 gcgcgctcag tgatataacg aacatccagc cctcgctcga gagcgatttt tccgctgaaa
     3661 gtaatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac gatatttaat
     3721 ttgccgtcgg taatttctgt ttttaaactg cccgcagtta atgtgccgca gaaaatgatt
     3781 ttcttcgaag tggcactgat atcaataaat ccaccggtgc ccatgatttt accattgaat
     3841 ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc
     3901 agaccgccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc acgggtattc
     3961 acgttcgcgc caaaggcgat cccctgtgaa gtaattccgc caatcggacc tgtttctacc
     4021 gtcagaataa agtcatcagc acaaccttct tctcgggcga ccaggccaat gccgtcagca
     4081 ataccgacgc cgacattccc caccgcgcct ttacgcattt cgaataatgc gcgccgcgca
     4141 actaatttac gttggtttag gggcaggcta agtttggtac tgtcatcaag ggtgaagtca
     4201 ccagaaataa agcggttaac cggtgcaccg ccatacagtt gggtttgatc cggatcgacc
     4261 accacaatat ccaccagata acccggaata cggacagatt taggatgcag cgtggctttc
     4321 ttaaccattt tctgcacctg catcatcaca ataccgccgt tattgtgcac cgcctgggca
     4381 ataaccaatg cgtcgagata catcacctca tcttcaaaag tggcgtagcc ttcgctgtcg
     4441 caggtggtag cgcgaatgaa ggcgatatct ggcgcaatcg ctttgtaata gagatattct
     4501 ttgttatcaa actcgaccag tttaatcagg tcttctttag tgacttcatt cagtttgccg
     4561 ccttgctggc gtggatcgac aaatgtcccg atgccaatat cactaataat accaggcggg
     4621 tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt gtggatagtt ataagcaata
     4681 attttatttt gttctgcgag atcagaaata cgcggcgatt gtccccagtg accgcataat
     4741 gcccatttca gcagaccttc ttgcgccagc ggactaatac cgcggtcggc gcgatcgcca
     4801 agccctgttg gactaataat cgataaatta cgtggcgttt gagtctgttt atatttatca
     4861 gcaagagcag taattaacgt ggtggcttcc agaataccgc cgccagcgcc taacacacaa
     4921 agtgttgctt cgtcgggaat ataattcacc gcttcctgtg ccgacaggac cggcactcgt
     4981 ccattaatac gaggtggttt tacaggtttc attgttaatt cctgattcag gtgtgatttt
     5041 ccctacgcca gatggataat tagcgcacag agactcacga aatattttta ttaaatacgg
     5101 ttgaatatat ttttttagtt cggcggggga ggatattccc gccgaaatat tattgcttat
     5161 gcctgatgta aaatagttaa tactgtgcgt aaatcagtta ccgagatttg ccctggcgca
     5221 gaggcttttt ttaccgcacc aaaagttgcc gccgacccaa atacttcacc agccagacga
     5281 gaaattacgc cagtttttgc catcgacatc gtgatgattg gacgatcggc atactgctcc
     5341 tgcatctcca gggtcgcggc aagcaacgtc agcacatcgc tggtactttg cggcatcagc
     5401 gcaatcttag gaatatcggc gtcgaaggat tgcattttgc gcagacgggc aatgatttct
     5461 tcggcttccg gcgttttatg gaagtcatgg ttggacatga caactttcac atcatgcgcg
     5521 tgggcgtagg cgacggtttc tttaacctga tcatcgccgg taaataactc cagatcgatc
     5581 atatcaacca ggccgctgtc gatggctgca cgattgagag caatataagc ctcggtggaa
     5641 atcgcctgct cgccgccttc tttggcactg cggaaggtaa acagcagcgg tttttctggc
     5701 atggtttcac ggagaatttt tgccgccgcc atgacagact ccacattgga gaggtcggca
     5761 aagtggtcca cacgccattc cagaatatca aagtccgctt cacgataggc gagagcttcg
     5821 gatttcacgc gggcgatatc tttcgccatc agcgagacga tgattttagg tgcgcccgca
     5881 ccaatgacga gatcttttac agttacggtt ttcatttttt accctttctg cacgcggtca
     5941 gcctgtcagg caccgaaccc catgacctgt ttaacatatt ccagagggaa atctttgcca
     6001 gtccataacg tgaactgttc agccccttgc cacaacaaca tgccgtatcc atcaatcgtt
     6061 ttgcaaccag cttgttgcgc ctgctgcaat aacttcgtca tatgcgggtt atacacgcat
     6121 tcagtgacca gcagtcccgg atgtaacaga ctgatatcat taaccaatga ttcattctca
     6181 aggggtttca tacccacttt tgtgccattg gttaaaatgt cggcggaagc cagggcttca
     6241 gcaaaggctt gctgatcggc gagatcggta accgtgacga cacaatcggt gttttcgtta
     6301 acccgctgcg cgaaggcgag ggctttatcg aagaactcat cccgacggtt aaagagttta
     6361 atttctttta aaccttcaat tgccccctgc gcgccaatcg ccgttgaggc acctccggcc
     6421 cctaacagca ccattgtttt gcctttgata tcaaaaccgc tctctttaat ggctcgaata
     6481 tggcccgtgc cgtcggtgtt atagccgcgc aggtagccat catcattaac gatggtgttg
     6541 atggcaccca ccagtttggc tgctggcgtt aattcatcaa catattcaca cgccagttgt
     6601 ttgttcggca ttgatacacc agttccgcgc atttttagag cttttaatcc ttcaattgct
     6661 ccgggaaagc tatcgttatc cacttcgaag gccatatagg taaatggcaa tcccgctttt
     6721 tctaaggctt tattctgcat ttcaggcgat aaactgtggc ggataggata agccatcaac
     6781 ccaatcaatt cgtattttgc ggtaacatcc ataattaact cctttaatct ctatgcttaa
     6841 ttgtgtttaa ccgggtgcta aatttgcgct cgccaaaccg cacatcattc tcaggaataa
     6901 taaaaacgcg gtaatagcgg ataaaaacaa taattgcggt aatcagggcc agcagcgcga
     6961 aagtaaaatc gagaacaatg atatattgca ggccgatgtt cgacagataa ccggtaatca
     7021 gcggaataac aaagttagcc agtccaccca tcatcatata aatactggtg actttggctt
     7081 tgctttttgg gaaaaactct gacatgaccg aaacaccgag ctgtaaaatg ccgccagctg
     7141 ctgagaaacc gataacaaag gcgccggcat tacacaccag tggagaaggg tacaggtaga
     7201 taatggctgc tgttattgtt gccagtgcag agttaaatac attagcccag atgggccgga
     7261 ccattttttt cagtagtgcg gcaaaaataa agacacagac caacgagccc atactgtaat
     7321 aagagatggt ttttaatgcc tcagcttctg acataccagc aaaagccatc gcatatttgg
     7381 gcatccacac cacaatcaca taaaaggtcg agaatgcggc tacaccgaac agtaccgatg
     7441 aaacaccttc cagccagact aacggtttgc tgttcatttg cggtaattca ttagttacgc
     7501 tggcgtccac caactggctg gggaatttgc ttttcaacag catcagcgtg atcagtacaa
     7561 atagaatacc cggaataatc agcccatagc cgtaccagat attattgagc aacatatagc
     7621 tcaccagcat tgggtagaac atttgcccaa atgacaccat cgctttaacc agtatgaccg
     7681 ccgaaccaga ggctttcgga aagcattcca tgagcgcggg gtagccaccc gtatccagcg
     7741 ctgagttagc gatacctacg cacactgcca gaccgtaggc gagagttaaa ttcgggcaag
     7801 cgggaatacc aaagaagaat agcagataca ttattactgc cattaatatc accgcccgac
     7861 gaccaaactt atcggagatc acaccgaaga ataaaatact gatcaatcgc cccaaaccga
     7921 taccggaaat taagtaggca atgcccgcgt tgtcagtgga aaacttttcc gccagagatg
     7981 acatattttg ggcaagcgta ataacactaa tgccgtgcag gaagtagcag aagtaaatac
     8041 aaagaacagc caggataaat ggcgtgctga aagccttatt ttgagacatt ttcacagctc
     8101 cttgctgaaa gataatttcg ttaccgtacg tattgttctc cgacggttta cataattact
     8161 atactgaaga tgattttccc tgggtacatg gcataaagtt tatgtttcca ggaatattgc
     8221 atgtcatagt ttgtttttga tcgcgatttt ccttagttag cgcagaaaga gttatttatc
     8281 cgtaagatct tgcatgacct gaaaagaata ttgcattacc cacccggagc gacattttcc
     8341 tttagcgagt gatgccgcgt ttttttgcgg ctgcgtaagc cgataaacag tgccagtaaa
     8401 aaaccgatgg cagcgatggc ggtatcgaac cacataatat cggcaatact tctttgggac
     8461 agatgagccg taatcaacgg aatagtaaag gtcgcaatac tgcccgcact gtaatagatc
     8521 cctgtagctt tacctttagc gtaagggaaa cgttcagcca ttaacgtcag gccaatttgc
     8581 acaacacctc cagcagaggt aaaaccaatg acaaaagcaa atattatcac cacataaaat
     8641 gtgggatgca ggcagacggt aaacagagca ataaatgaga taaaggtgta cagcatcagt
     8701 aatgttgttg ggcgaacggt attacgaatg agtggagcgg taataaatac acaaagcagc
     8761 gaacccacgg tgtagatact gagtagtttg atcgacatag tgtatgacat gcctgcaaca
     8821 aattgtccgt actgtgccag ccactggcta accagataaa acgttgccat tgagatatag
     8881 ccatataagg tataactggc taaatcgata attgaacagc gatgttccgt agagctggtg
     8941 gttttcttta tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca acggtataaa
     9001 aacagagcgt taataaacat aatgcctgca gcaatcataa aggaccaacc gaaccacagt
     9061 tcagcccaca ctaacaggct aatgattagc ggtaataaaa attgtccgct ggaaacaaat
     9121 gctttaatta aaatattggc tgtcccaggt gagcgtggaa aagcttccat caaactggga
     9181 taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa aacataagcg
     9241 ataatgatgt tattggtctg caggatgcca aaaaagaagg ccatatagca gcacatcccg
     9301 agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa aagcaagaca
     9361 cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc attagtctgc
     9421 caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat accatgcacc
     9481 aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcgt aggttctact
     9541 tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccgttca ttattgttgt
     9601 aacaacgaca attccttttt gtggagaaat taacaaagcc gctccagatc gtgtgcatat
     9661 aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc agggtagact
     9721 tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt
     9781 attttctcta tgatcgcatg aagatgtttt tttctttgct gacgcatagc caacagttgc
     9841 tcgactacac aatcaggaat ggcacacttg ccattttccc attgacgcca gctttcactg
     9901 tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg
     9961 agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa caataaaata
    10021 taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt gtgcaacatt
    10081 attggaaaga gtgaggggcg aggtagcgat gcggaaaaat gttaaaaaaa agcccatcgt
    10141 gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga tttccatgct
    10201 tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg actttttgcg
    10261 tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag
    10321 ctgaatggcg gtaagcagga aaatatgggg aagagcaagc aaatatcaat tagcggtggt
    10381 taacaatcag gcggcagagc cgcctgatga gaaattactt attcggtttc tcgatttcgc
    10441 caagttcttc gagaatttct tccggttggt ccgtcggcgg cgggacttct tccacccacg
    10501 cggcaaacag acgccaggaa acggctaaca gaaccggacc aataaacaga ccgatcatcc
    10561 cgaaagcaat caaaccacca ataacgccag agaggatcag gatcagcggt aaatctgcac
    10621 ccatgcgaat taacattggg cggatgacgt tatccagcgt gccaaccaca ccgctccaca
    10681 ctaacaatac cgttccccag gtggtatcgc cggtccagta gagccagata atcgccggaa
    10741 tcagtaccgg caacgggcca agctggacaa ggcaggagag gatcattaac accgttagca
    10801 aagttgcata aggtacgccg gaaacggcga ggccgatacc gccaagcact gcctgtacta
    10861 acgccgtcac caccacaccc agcgccaccg cgcggatagc ctgtgccgcc agcagtacgg
    10921 cggcatctcc gcgaacgcct gccagacggg tcgcaaaatg gcgaatgcct tgtgccacct
    10981 gttcaccgcg ccagtacagc agggcactga agagaagcat cagcgcacaa tgcaccataa
    11041 agcgcccgat atgcgccgct tgcccaacga accaggtggt ggtggtgcca atataagggc
    11101 ggactttcgc catgatcgcc gtccccccca tatccagcaa gttgtgccag cctgcataca
    11161 gcttcgcgcc aatcaccgga atggtattaa gccacgccag atcgggtaac gtcatgtcac
    11221 cgctggaaat ggctttaatt agcgggccgc tgccgtcgac gatactgtta accagcaaag
    11281 caatagggat gataaacacc atcactaata acagcgtcat caccagaacg gcgagagagc
    11341 ggcggccaaa catgatcttt tgcaaacgta acaataccgg ccaggtggcg ataaccaccg
    11401 taccggccca tgcaaagccg agaataaagg gttgaacaat ccacagacat gccacaatca
    11461 tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac atccctgggc tgacgaacat
    11521 ttaccataac tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc
    11581 gaaactcacc tgcataatga tgagcaattt cagcggtttt taacaggccg attctgccct
    11641 taattctgat gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg
    11701 atttactcgc cgtatttcaa gttgcatgtg ctgcggctac gttcgttcac cccagtcact
    11761 tactattgta agctcctgga gttcactcgc ttgccgcctt cctgcaacac gaaatccata
    11821 gagtaaacaa acatcacctt tcagtgcgct attgcagcag cctgaaagaa tagtgtatcg
    11881 acctacatca cagaccgcag gaaagggtca atataatgat tccacagatt tcccaggcac
    11941 ccggcgtcgt tcaactggtg cttaattttt tgcaagagct ggagcaacaa ggttttaccg
    12001 gcgatacggc gacaagttat gccgatcgtc tgacaatgtc gaccgacaac agtatttacc
    12061 aacttctccc cgatgcggtg gtatttccgc gttcaaccgc agatgtggcg ctgatcgccc
    12121 gtcttgccgc gcaggaacgc tattcatcgc tgatctttac cccccgcggc ggcggcaccg
    12181 gcactaacgg tcaggcgctc aaccagggga ttattgttga tatgtcccgc catatgaacc
    12241 gcatcatcga aattaaccct gaagagggct gggtgcgcgt tgaggccggg gtgataaaag
    12301 atcaactcaa tcagtacctg aaaccgttcg gctacttttt tgcgccggaa ctttcgacca
    12361 gcaaccgggc aacgctcggt gggatgatca ataccgatgc ttctggtcag ggatcgctgg
    12421 tctatggcaa aacgtcagat cacgtacttg gcgtacgcgc ggtgttgttg gggggcgata
    12481 ttctcgatac gcaaccttta cccgtcgagc tggcggaaac gctgggtaaa tccaatacca
    12541 caatcgggcg aatttataac acggtttatc aacgttgccg tcagcaacgc cagttaatca
    12601 tcgacaactt ccccaaactt aaccgctttc ttaccggtta cgatctgcgt catgtcttta
    12661 acgatgagat gaccgagttc gacctgacgc gcattctgac gggttcagaa gggacgctgg
    12721 cctttattac cgaagcgcgg ctggatatta cgcccttgcc taaagtgcgc cgtctggtga
    12781 atgtcaaata tgactctttt gactcagcgc tgcgtaacgc gccgtttatg gttgaggcgc
    12841 gggcgctttc ggtagagacg gtggactcaa aagtgctgaa tctagcgcgg gaagatattg
    12901 tctggcattc cgtcagcgag ttgattaccg atgtgcctga caaagagatg ctcgggctga
    12961 acattgtgga atttgctggt gatgatgagg cgctgattga cgagcgggta aatgcactct
    13021 gtgtgcggtt ggatgagctg atcgccagtc atcaagcagg tgtcatcggc tggcaaatgt
    13081 gccgtgagct ggcgggggtt gaacgtatct atgcaatgcg caaaaaagcc gttggtctgc
    13141 ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc
    13201 cggaacatct ggccgattat attgctgaat ttcgcgcgct gctcgacagc cacggcttaa
    13261 gctacggtat gttcggtcac gtcgacgcag gtgtcttgca cgtccgtccg gcactggata
    13321 tgtgcgatcc gcaacaagag attttgatga agcaaatctc tgatgacgtg gtggcgctga
    13381 ctgcgaaata tggtggtttg ttgtggggcg agcacggcaa aggttttcgc gctgaataca
    13441 gcccggcgtt tttcggtgag gaactttttg cagaactgcg caaagtgaaa gcggcatttg
    13501 acccgcataa ccgactcaac ccagggaaga tttgcccgcc agaaggtctc gatgcgccga
    13561 tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg gcagatcccc attgcggtac
    13621 gccagcagtg gcgcggtgcg atggagtgta acggcaacgg tttatgcttc aactttgatg
    13681 cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa ccggattcat tcaccgaaag
    13741 ggcgcgcaac gctggtgcgt gaatggctgc gtttgttggc ggatcgcggc gttgatccac
    13801 tcaaactgga acaagaactg cctgaatccg gcgtcagttt gcggacgtta attgcccgca
    13861 cgcgcaatag ctggcatgcg aataaaggcg aatatgactt ctcacacgaa gtcaaagagg
    13921 cgatgtcggg ctgtctggcc tgtaaagcgt gttcgaccca gtgccccatc aaaattgatg
    13981 tgccggagtt tcgctctcgt tttctgcagc tctatcacac ccgttattta cgtccgctgc
    14041 gcgaccacct cgtcgctacg gtcgagagct acgcgccgct gatggcacgt gcgccgaaaa
    14101 cctttaactt cttcattaac cagccgctgg tgcgcaaact ctcggaaaaa catatcggca
    14161 tggttgattt gccactgctg tcggtcccct cgctacaaca acaaatggtg gggcatcgct
    14221 cggcaaacat gacgctggaa cagcttgaag ctctcaatgc agagcagaaa gcgcgcacgg
    14281 tgttggtggt gcaggacccc tttaccagct attacgatgc gcaagtggtg gcggattttg
    14341 tccgtctggt cgaaaaatta ggtttccagc ctgtgttact gccattttca ccaaatggca
    14401 aagcccagca tatcaaaggc tttcttaatc gttttgcgaa gacggcgaaa aagacggcgg
    14461 atttcctcaa ccgtatggcg aagctgggta tgccaatggt aggcgtcgat ccggcgctgg
    14521 tactttgtta tcgcgatgaa tataaactgg cactgggcga ggaacgtggc gagtttaacg
    14581 tcttactggc gaatgaatgg ctggcaagcg cacttgagtc acagccagtg gctacagtca
    14641 gcggtgaatc atggtatttc tttggtcact gtaccgaagt taccgccttg ccgggtgcgc
    14701 cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa actggaaaat gtcagcgtgg
    14761 gttgctgcgg catggcaggg acttacggac atgaagcgaa aaaccatgaa aattcgctcg
    14821 ggatctatga gttatcctgg catcaggcta tgcagcgact gccgcgtaac cgctgtctgg
    14881 cgaccggata ttcctgccgt agccaggtaa aacgggttga aggcacgggg gtacgccatc
    14941 ctgtgcaggc tttactggag attattaaat gatatggaaa cggaaaatca ccctggaagc
    15001 actgaatgct atgggtgaag gaaacatggt ggggttcctg gatattcgct ttgaacatat
    15061 tggtgatgac acccttgaag cgacaatgcc agtagactcg cggacaaagc agcctttcgg
    15121 gttgctgcat ggaggagcat ccgtggtact ggccgaaagt atcggttccg ttgccggtta
    15181 tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa atcaatgcta accacgtccg
    15241 ctcggcacga gaagggcggg tgcgcggcgt atgcaaaccg ttgcatctcg gttcgcgtca
    15301 ccaggtctgg cagattgaaa tcttcgatga gaaagggcgt ttgtgctgtt cgtcacgatt
    15361 gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg acgcatattg cttttggtca
    15421 ttatctgcac agtaaacggt ggtatactgt gcaggtcttg tctaaaagcg aggatgctat
    15481 gtctactcaa cttgatccca cccagctggc cattgaattt ttacgccgtg accagagcaa
    15541 tctttcacct gcccaatatc tcaaacgtct gaaacaacta gaacttgaat ttgctgacct
    15601 gcttactctc tcttctgctg aattaaaaga agaaatctac tttgcctggc gtctgggcgt
    15661 gcattgatcc tgacttaatg ccgcaagttc gccaaatcga cctggattaa cctccgtcca
    15721 ggttcgatga ccttcttaat actgcttatc tatcgcctga tattgttcaa aaataatact
    15781 aatcgcagtt tgtgttaaaa cggcgggtta gctttatgag attattctta tcgccccttc
    15841 aagagctaag ccactgagag tgccggagat aagcgccgga tggggtagaa acccttaagc
    15901 ctgtgtcgca cagacttaag ggttttctta tttctgaacg ctgcactttt ttacctatct
    15961 cttaaatagt cattttcaat acaacttttt ctttttcatc acagccttaa tgctccttag
    16021 ataataacca ttatctaaca atgagatacc taattcttag cgtgcctgtt aacccacaca
    16081 tcagggtcta tgcttattaa aagcaggcaa aaagcatgtt ttacggtaaa gcccctgcgt
    16141 ttgctgggtt gaactgataa tcattatcac taacatgctg ttatacgctg aaagcgatga
    16201 agtgaggtaa atcgatggac atgcattcag gaacctttaa cccacaagat ttcgcctggc
    16261 aaggcttaac gttgacaccc gcagcggcga tacacatccg cgagctggtg gcaaagcagc
    16321 caggtatggt cggcgtgcgc ttaggcgtga agcaaacggg ctgcgcgggc tttggctatg
    16381 tgctcgacag tgttagcgag ccggacaaag acgatctgct gtttgaacac gacggcgcga
    16441 agctgtttgt cccgctgcaa gcgatgccgt ttattgatgg cacggaagtc gatttcgttc
    16501 gtgaaggact taatcagata ttcaaatttc acaaccctaa agcccagaat gaatgtggct
    16561 gtggcgaaag ctttggggta taggcggtac tatgtctcgt aatactgaag caactgacga
    16621 tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa ggattcttca cccagttagc
    16681 caccgatgag ctggcaaagg ggataaacga agaggtggtg cgcgcaattt cggcgaagcg
    16741 taatgagccg gagtggatgc tggagtttcg tctcaatgcc tatcgcgcat ggctggagat
    16801 ggaagagccg cactggctga aagcgcacta cgacaagctg aattatcagg attacagcta
    16861 ctactcagca ccatcgtgcg gtaattgtga cgacacttgc gcgtctgaac ccggcgcggt
    16921 gcagcaaact ggcgcgaacg cctttttaag taaagaggtg gaggcggcgt ttgagcagtt
    16981 gggcgttccc gtgcgggaag gcaaagaggt ggcggtggat gccattttcg actctgtttc
    17041 ggttgccacc acttatcgtg aaaaactggc ggagcaggga attattttct gttcctttgg
    17101 tgaggcgatc cacgatcacc cggaactggt gcgtaaatat ctcggcaccg tggtaccggg
    17161 gaatgacaac ttctttgccg cgcttaatgc ggcggtagcc tctgacggta cgtttattta
    17221 tgtgcctaaa ggtgtgcgct gccctatgga actttccacc tattttcgca ttaacgcgga
    17281 aaaaaccggg cagtttgagc gcaccattct ggtggccgac gaagacagct acgtcagcta
    17341 cattgaaggc tgttccgctc cggtgcgtga cagctatcag ttacacgcgg cagtggtgga
    17401 agtcatcatc cataaaaacg ccgaggtgaa atattccacg gtacaaaact ggttccctgg
    17461 cgataacaac accggcggta ttctcaactt cgtcaccaag cgtgctttgt gcgaaggcga
    17521 aaacagcaaa atgtcatgga cgcaatcaga aaccgggtca gcgattacgt ggaaatatcc
    17581 cagctgcatt ttgcgcggcg ataactccat tggtgagttt tactcagtgg cgctgaccag
    17641 cggtcatcag caagcggata ccggcaccaa gatgatccac atcggtaaaa acaccaaatc
    17701 gaccattatc tcgaaaggga tctctgccgg acatagtcag aacagttatc gcggcttagt
    17761 gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact cagtgcgact caatgctgat
    17821 tggcgctaat tgtggggcgc ataccttccc gtatgttgag tgtcgtaaca atagtgcgca
    17881 actggaacac gaggcaacga catcacgtat tggtgaagat caactgtttt actgcctgca
    17941 acgcgggatc agcgaagaag acgccatctc gatgattgtt aacggtttct gcaaagacgt
    18001 gttctcggag ctgccgttgg aatttgccgt tgaagcacaa aaactcctcg ccatcagtct
    18061 tgaacacagc gtcggataag gaataaacat gttaagtatt aaagatttac aagtcagcgt
    18121 ggaagataaa gctatcctgc gcggattaag cctcgacgtt catcccggcg aagttcacgc
    18181 cattatgggg ccaaacggtt cgggcaaaag taccttatcg gcaacgcttg ccgggcgaga
    18241 agattatgaa gtgacgggcg gcacggttga gttcaaaggc aaagatttgc ttgcgctgtc
    18301 gccggaagat cgcgcgggcg aaggcatctt tatggccttc cagtatccgg tggaaattcc
    18361 cggcgtcagt aaccagtttt tcctgcaaac ggcacttaat gcggtgcgca gctatcgcgg
    18421 ccaggaaacg ctcgaccgct ttgattttca ggatttgatg gaagagaaaa tcgctctcct
    18481 gaagatgccg gaagatttat taacccgttc ggtaaacgtt ggtttttccg gcggcgagaa
    18541 aaagcgcaac gatattttgc aaatggcggt gctggaaccg gagttatgca ttcttgatga
    18601 gtcagactcc gggctggata ttgacgcatt aaaagtggtt gccgatggcg tgaactcgct
    18661 gcgtgacggc aagcgttcat tcatcattgt tacgcactac caacgcattc tcgactacat
    18721 caagcctgat tacgtccatg tgctgtatca gggacgaatt gt
//
LOCUS       DACAPB010000081        18536 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.081, whole genome shotgun sequence.
ACCESSION   DACAPB010000081 DACAPB010000000
VERSION     DACAPB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.081"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..331
                     /locus_tag="IT904_002517"
     CDS             <1..331
                     /locus_tag="IT904_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="AraC family; binds to the right arm of the
                     replication origin oriC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAP3192284.1"
                     /translation="LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEY
                     VMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINLRCE
                     LLIPIRR"
     gene            complement(328..975)
                     /gene="gpmB"
                     /locus_tag="IT904_002518"
     CDS             complement(328..975)
                     /gene="gpmB"
                     /locus_tag="IT904_002518"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAP3192285.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1027..1539
                     /gene="yjjX"
                     /locus_tag="IT904_002519"
     CDS             1027..1539
                     /gene="yjjX"
                     /locus_tag="IT904_002519"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAP3192286.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(1686..2012)
                     /gene="trpR"
                     /locus_tag="IT904_002520"
     CDS             complement(1686..2012)
                     /gene="trpR"
                     /locus_tag="IT904_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAP3192287.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2102..4039)
                     /gene="sltY"
                     /locus_tag="IT904_002521"
     CDS             complement(2102..4039)
                     /gene="sltY"
                     /locus_tag="IT904_002521"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAP3192288.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            4250..5917
                     /gene="ettA"
                     /locus_tag="IT904_002522"
     CDS             4250..5917
                     /gene="ettA"
                     /locus_tag="IT904_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAP3192289.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6225..7457)
                     /gene="nadR"
                     /locus_tag="IT904_002523"
     CDS             complement(6225..7457)
                     /gene="nadR"
                     /locus_tag="IT904_002523"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAP3192290.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(7478..8860)
                     /gene="radA"
                     /locus_tag="IT904_002524"
     CDS             complement(7478..8860)
                     /gene="radA"
                     /locus_tag="IT904_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP3192291.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(8909..9877)
                     /gene="serB"
                     /locus_tag="IT904_002525"
     CDS             complement(8909..9877)
                     /gene="serB"
                     /locus_tag="IT904_002525"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAP3192292.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            9983..10627
                     /locus_tag="IT904_002526"
     CDS             9983..10627
                     /locus_tag="IT904_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAP3192293.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            10655..11671
                     /gene="lplA"
                     /locus_tag="IT904_002527"
     CDS             10655..11671
                     /gene="lplA"
                     /locus_tag="IT904_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAP3192294.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(12127..12846)
                     /gene="deoD"
                     /locus_tag="IT904_002528"
     CDS             complement(12127..12846)
                     /gene="deoD"
                     /locus_tag="IT904_002528"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP3192295.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(12926..14149)
                     /gene="deoB"
                     /locus_tag="IT904_002529"
     CDS             complement(12926..14149)
                     /gene="deoB"
                     /locus_tag="IT904_002529"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP3192296.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(14201..15523)
                     /gene="deoA"
                     /locus_tag="IT904_002530"
     CDS             complement(14201..15523)
                     /gene="deoA"
                     /locus_tag="IT904_002530"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAP3192297.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(15650..16429)
                     /gene="deoC"
                     /locus_tag="IT904_002531"
     CDS             complement(15650..16429)
                     /gene="deoC"
                     /locus_tag="IT904_002531"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP3192298.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            16687..18237
                     /locus_tag="IT904_002532"
     CDS             16687..18237
                     /locus_tag="IT904_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAP3192299.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            18209..>18536
                     /locus_tag="IT904_002533"
     CDS             18209..>18536
                     /locus_tag="IT904_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAP3192300.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNHCGECVPQCPHQALQIVDGKVVWNAAVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKA"
BASE COUNT         4270 a         5157 c         4826 g         4283 t
ORIGIN      
        1 cctgaatgaa acgcgtccga gtcaggataa agacgacgaa caagaggtat tctataccac
       61 cgcgttagcc caggatcagg cagatggcta tgtactgacg gggcatccgg tgatgctgca
      121 gggcggcgaa tatgtgatgt ttacctatga aggtctggga accggcgtgc aggagtttat
      181 cctgacggta tacggaacgt gcatgccaat gctcaacctg acgcgccgta aaggtcagga
      241 tattgagcga tactacccgg cagaagacgc caaagcggga gatcgcccaa ttaatctgcg
      301 ctgtgaactg ctgattccta tccgccgtta acgctgcagc tcatctaatg caggggcgtc
      361 cagatgcgag atgtcccctg cggtttcaac gacccagcca gacgccagcc acaggctttc
      421 ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct ctgcccatgc
      481 tggtaatccg agaatcgtac tcaccaggca tcccagtgca ataccatgac tcaccagcag
      541 cggtcggctt ccctgcggta agtcccggca agattccagt gccgcattga cgcgatcgct
      601 gagctcctgc attgactcgc cttcaggaat acgcccgtca acggtgccat tgaccagctg
      661 ccgacgccag ttctcttctt cttcggtcag agaatcgata tgtctttttt ccagcacacc
      721 catgtttaat tcacgcaggc gagaatcaaa gatgatgtca cagccgcagg cctgggcgat
      781 gatttccgcc gtacgccggg tgcgccctaa atcgctactg atgatatgcg taatgccaag
      841 ctctttggca cgcgtcgcca cctgcatcgc ctgttgctca cctttggcgg tcagcgggct
      901 gtcagactgg ccctgaatac gtcgctcggc gttccactgc gtttcaccgt ggcggactag
      961 gtatacctgt aacatgcttt ttttccgtat actgctgtaa cgttaatttt ctgagtaatg
     1021 ctgattatgc accaagttgt ctgtgcgacc accaatcccg ctaaaattca ggccattctg
     1081 caggcatttc acgagatctt cggcgaagga tcctgccata ttgcatccgt tgccgtcgag
     1141 agcggtgtac cggaacagcc ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg
     1201 gtagcaaatg cccgccgttt acttccagag gctgattttt gggtggcgat tgaagctggc
     1261 attgatggcg acagcacttt cagttgggtg gtgattgaaa acaccagcca gcgcggcgag
     1321 gcgcgttcgg caactttgcc gttgcctgcg gtgatattgg aaaaagtacg tgaaggtgag
     1381 gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg agattggtcg taaggaaggg
     1441 gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca gcgtctatca ccaggcggtg
     1501 atcctggcgc tgagtccgtt tcataatgcg gtgtattaag cgcctgatgc gacgctgccg
     1561 cgtcttatca tgcctaccaa acatattgaa attacgggta tttgtaggac ggataaggcg
     1621 ttcacgccgc atccggcatt cggtgcacga tgcctgatgc gccacgtctt atcaggccaa
     1681 caaaatcaat cgcttttcag caacacctct tccagccact ggcgcagctc aacgggcgcg
     1741 gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt
     1801 aactcacgct ggctcatttc gccgcgcaac agctcttcga caatacgcac gcgagtcccc
     1861 aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca acggtaaatg gagatcgttt
     1921 tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc
     1981 atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta gcgggggaag
     2041 caaaatgcct cccccggttg tactcgctaa agagtacgat agcatatcat aaacgtgcgg
     2101 atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat
     2161 aacggtagta agcgtcataa gccagcacgt tcttcacata accgcgcgtc tcggagaacg
     2221 gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgttgcca agccaggttc
     2281 gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta ttgccaaact
     2341 gctgataaac atattgcagg taactggtgc caatgttgat attcgtttcc ggatccagca
     2401 attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg gtcgctgtac
     2461 caggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc ggattccagg
     2521 cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctctttc ccactggtgt
     2581 aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga tcccaaagct
     2641 tcccggcgat cgttgcctga acgctaagat cccaccattg gttattgaaa gcataccgag
     2701 ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac tcgctacgcg
     2761 cggtgttatc gagattccag tacatcaact cgcgcacgcg cgccatctcc ggcccctgag
     2821 tcagggcgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac tcttctccga
     2881 tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg catgagttga tgcaaaatct
     2941 ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc cgcctgccag taacgccatt
     3001 catctttctc ttttgcttcc atcggcagac gcgccagcca ggtattcagg ccgcgacgat
     3061 cgccggtgcc aagtgccatt cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca
     3121 taatggcgtc atcgcgccat ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc
     3181 aggcgacgat atcgcgcagc tcctgaatct gatcttcatt aagctgctgc gcctgggcaa
     3241 gcgacgggat catcagccgt gcattttcag catcctgccg cgccacactg gcaaacgcca
     3301 ccgctgccat ttgacgggta aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg
     3361 tattagggtt attcgccagt gaaatgattg ccgaggcgat agtctggtaa tcggcaggca
     3421 tctgccctgc cagcactgtt accaggccag tgttacccgc tttcatcgcc agacggatac
     3481 gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta aataacttgt
     3541 cacaggcatt aggctggctc ttgccggtta gccacagctc tttcgcccct tgccaggctt
     3601 cttcactctg cccggtgttc catttcgcat agtagtaatt acattgcgct tcggtagttc
     3661 cgggcttttc cgggctaaag gctaacaagc cacgccagtc ttcacgccgc gccagttcat
     3721 tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt
     3781 tagtgaccgt caccgccggt tgattcatca gatcatcggt gatctggcgg tattccaggt
     3841 agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca tccatctgtc
     3901 gattatccca ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg
     3961 ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc cgccaggtaa
     4021 cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat
     4081 gccgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta cggacacttt
     4141 tttactttta ctgcgaaggt gatcgaccaa ctgggattag gcggggaaaa aggctacact
     4201 tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg tggctcaatt
     4261 cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa
     4321 catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga acggcgcggg
     4381 taagtctacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag gtgaagcacg
     4441 tccgcagcca gacatcaaga ttggttatct gccgcaggaa ccgcagctga acccggaaca
     4501 caccgtgcgt gagtccattg aagaagcggt ttctgaagtg gttaacgccc tgaaacgcct
     4561 ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc tggccgctga
     4621 acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataacctga acgtacagct
     4681 ggagcgtgcg gcggatgcgc tgcgtctgcc ggactgggac gcgaaaatcg ctaacctctc
     4741 cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctt ctggaaaaac cagacatgct
     4801 gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcctggc tggaacgctt
     4861 cctgcacgac ttcgaaggca ccgttgtggc gattactcac gaccgttact tcctcgataa
     4921 cgttgcaggc tggatcctcg aacttgaccg cggtgaaggt attccgtggg aaggcaacta
     4981 ctcctcctgg ctggagcaga aagatcagcg cctggcgcag gaagcttcac aagaagcggc
     5041 gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta aaggccgcca
     5101 gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg aacagcaccg aatatcagaa
     5161 acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg ataaagtgct
     5221 ggaagtcagc aacctgcgta aatcctacgg tgatcgcctg ctgattgatg acctgagctt
     5281 ctcgatccca aaaggggcaa tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac
     5341 cctgttccgt atgatctctg gtcaggaaca gccggacagc ggcaccatca ctttaggtga
     5401 aacggtgaaa ctggcatcgg ttgatcagtt ccgtgactca atggataaca gcaaaaccgt
     5461 ttgggaagaa gtttccggcg ggctggatat tatgaagatc ggcaacaccg agatgccaag
     5521 ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac gcgttggtga
     5581 actttccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa
     5641 catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc gcgcgctgga
     5701 aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc gttggttcct
     5761 cgaccgtatc gccacgcaca tcctggacta ccaggatgaa ggtaaagttg agttcttcga
     5821 aggtaacttt actgagtacg aagagtacaa gaaacgcacg ctgggcgcag acgcactgga
     5881 gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg
     5941 tgaacgcctt atccggccta caaaccaaga aatttcaata cgttgtgaat ctccaggcct
     6001 gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat gcggcgtgaa
     6061 caccttttcc ggtctacaac agccttagca tccgcaattt cgtagcgggt catccgtgct
     6121 ggttctttca aaccttgttt aatctcgaca gcctcttcca gagaggtttt caactcatca
     6181 aaaaaactca ttttatacct cgcctttgag ccgtttcatc gcggttatcc ctgctccccc
     6241 atcatctccc gcaccagctc cacgcagcga aggaaacggc tgtcataatc gtcctcttca
     6301 acccgcacga attcgatatt gttctcttcc agcatctcca ccagcaagtt ctggaactct
     6361 ttgcgatcca ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc
     6421 tccagcagga tcaccagatc gaaacggtat tcatcaatca acgcctgtac gaacggatgc
     6481 tcacgccctt cgtacttttt gcagaacgcc tgggtagtga caaaatcagt gtcaataaac
     6541 gccactttat tggcatattt cactgcaaaa tcaatgtatt gtgcgtggcc cagcgcgatt
     6601 ttatcgtaat cggaatactg caatgcgatc tcatcaccgc cgaggtgtga aaagacataa
     6661 tcgcgaccat attcccacgc actggtggta ttgaagatat tggcaagttt gtttaccagg
     6721 gtggatttac cgctcgactc gccaccaagg atcgccacgg tacgtacaaa gaacggcttc
     6781 acttccgtag gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg
     6841 atactcataa aggtacgttt cggatcgacc agcaccgtat cgatccccag atgttccata
     6901 tactgtggcg catcggcttc ttccgaggtg tagatcaggt ccggctgaat ccccttttca
     6961 gccataaact ttttgatgcc gttgctccac acatcccagc cgtgcggata cggctccatg
     7021 ccctcttcgt tgaaagcatg aatgcgaata tttttctgat atttaaaagt ttgtaataac
     7081 cagcgcagac gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg
     7141 cggtcgcggg tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg
     7201 cgctggataa ggtagatatg tccggtatgc agcgggtaga acttaccgaa tacgacacca
     7261 atggttttct tctgtcgggg aaattcaagc cccaaaaaac ggtgcaacgc ctccagcttt
     7321 tgcgcgctgg ggcttttaat tttggcattc agtaactggc ttaaataccc tttggtcata
     7381 ccgctggcat cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg
     7441 taatcaaatg acgacatatc tccctccgta tctctcatta taagtcgtcg aacacgctaa
     7501 gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggta
     7561 cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca gagattcgtt
     7621 cctgaccgct gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca
     7681 gatcctgtgg cagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaagt
     7741 cggcgctggt ttccgttacc ttcacgccgc cgaccacgtt cacaaacaca tcctgatcgg
     7801 ccatttgcag accaccgtga cggtgcaaga cagccagcag gatcgccaga cggttttgct
     7861 ccagacctac cgccacgcgg cgcggattcg ccatcatcga gtgatcgacc agcgcctgga
     7921 tctccaccag caacggacgt gttccttccc ataccaccat cactgaacta ccagaggtca
     7981 cttcatcgcc acggcttaaa aagatggcgg aagggttgct gacttcacgc agcccctgtt
     8041 cggtcatcgc gaagacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgta
     8101 aggtgcgaaa acgggagtcg gcatcgccat ccagaagcac cgaacagtcg atacagtgtt
     8161 ccagcacttt cgggccagcc agcgagccat ctttggttac gtgccccacc atgacaatcg
     8221 ccacaccgcg cgttttggcg aagcgtgtca aataagccgc cgtttcacgc acctgcgcca
     8281 cgctaccagg cgatgactgt acatccgcca tatgcatcac ctggatggag tcaattacca
     8341 tcagcttcgg ttgctcttct tcggcaatca ggcagatctg ctcgatgctg gtttccgaca
     8401 acatattgag attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca
     8461 gcgactcttc gccggtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt
     8521 gcagcaacag cgtggatttc cccgcgcctg ggttaccgcc aatcagaatg gcacttcccg
     8581 gtaccacgcc gccgcctaat acgcggtcga actctttaaa tccggtggaa aaacgcggca
     8641 gctcttcaag gctgatgtcg gagagtttct ggacttttgc caccccggcg ctaccggcat
     8701 agccactgag acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc acctcggtga
     8761 tggtgttcca ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc
     8821 attcattaca aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg
     8881 tgccgcccgc aggatgacgg gcaggcaatt acttctgatt caggctgcca gagaggatgc
     8941 agaatacccc catcaggtca gcgtgacgga tggtgacttc cgtcttttca ttcacttttg
     9001 gcttggcatg gtaggcaatc cccagccctg ccgctttgat catcggcagg tcattggctc
     9061 catcgccaat cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgggtca
     9121 gagttttcgc tttgtactgc gcgtcaacga tgtcgccgat cacattgccg gtaaatttac
     9181 cgtccatgat ctccagttca ttggctacca cggcggtcag gcgcagcttg tcgcgcaggt
     9241 attcagcaaa gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca
     9301 gcttgagcac cagctgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca
     9361 gaatattggc atcagcgcct ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat
     9421 cgagttcgcc gcgcatcgcc cgttcggtta cctccgccac catctcgccc gttccggcca
     9481 gtttggcaat ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca
     9541 gcaaacccgg cgtgcgcagg tgcgggattt tccccagcgg cgcgacatcc agttgcgctt
     9601 catgggccag gcgcgtagcg cgcgcggtga gtgaacctgc cagacgaatc acctgataat
     9661 cttccacgca ccaggcggca acaatcacca tcgccgcgcc cagtttgctc tggtattggg
     9721 tcaggcgttg tttatccagc ccacgaccat acagcagcca gccgctacga cctgcgtggt
     9781 aatccagtgg catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga
     9841 catcttcagg caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg
     9901 aagcagggaa aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc
     9961 tcaaattttc aaagggtgga agatggctcg cacaaaactg aaattccggc tgcatcgggc
    10021 agtgattgtc ctgttctgtc ttgccttgtt agtggcgctg atgcagggag cgtcatggtt
    10081 tagtcaaaac caccagcgac aacgtaatcc acagctggaa gaactggccc gcaccctggc
    10141 gcgtcaggta acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg
    10201 cattcaggcg atccttgatc agttaacgga tgaaagccgt atcctcgacg cgggcgtgta
    10261 tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc gcgaccggct
    10321 ggcgctcgac ggtaaaaaag cgggcggcta ttttaaccag cagattgtcg aaccaattgc
    10381 cgggaaaaac ggaccgctcg gctatctgcg cctgacgctc gacacccata cgctcgccac
    10441 cgaagcccaa caggtggata acaccactaa cattttacgc ctgatgttgc tgctttcact
    10501 ggcaatcggc gtagtgctga cccgcacgct gctacagggt aaacgtaccc gctggcagca
    10561 atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa
    10621 agagtgaccc attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc
    10681 tgactcttac gacccgtggt ttaacctggc ggtggaggag tgtatttttc gccagatgcc
    10741 cgccacgcag cgcgttctgt ttctctggcg caatgccgac acggtagtga ttggtcgcgc
    10801 gcagaacccg tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc
    10861 acggcgcagt agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt
    10921 tatggctggc aagccggagt acgataaaac catctccacg tcgattgtgc tcaatgcgct
    10981 gaacgcgctt ggcgtcagcg ccgaagcgtc cgggcgtaac gatctggtgg tgaaaaccgc
    11041 cgaaggcgac cgcaaagtct caggatcggc ctatcgcgaa accaaagatc gtggcttcca
    11101 ccacggcacc ttgctgctca atgccgacct tagccgcctg gcaaactatc tcaatccgga
    11161 taaaaagaaa ctggcggcga aaggcattac gtcggtacgt tcccgcgtga ccaacctcac
    11221 cgagctgttg ccggggatca cccatgagca ggtttgcgag gccataaccg aggccttttt
    11281 cgctcattat ggcgagcgtg tagaagcgga aatcatctcc ccggacaaaa cgccagactt
    11341 gccgaacttc gccgaaacct ttgcccgcca gagtagctgg gaatggaact tcggtcaggc
    11401 tccggcattc tcgcatctgc tggatgaacg ctttacctgg ggcggcgtgg aactgcattt
    11461 cgacgttgaa aaaggccata tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc
    11521 gccgctggaa gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca
    11581 acaagagtgc gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt
    11641 gtcgacgtgg atagcggggg cggtaaggta actcgctgat taaaatacca aaggaaggga
    11701 atatgatcga tctggaaaac caggagcggg agattatcaa tttaatgtta agccagcgta
    11761 tttcctggct agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa
    11821 tgttaggtat ctcgattaat tctctaaaat agatcgaaaa gacggaacgg tttagctcaa
    11881 acatcaaaag caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt
    11941 cctggatgac ggctgaacat aaatgaatag attcgtgaat agatacttga atagatttta
    12001 ttttctatcc atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga
    12061 tactaaaaag ccggagcagt ctcccactcc ggcttcacac gacaatcgcc tttcagcgaa
    12121 acacaattac tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt
    12181 gaaggtggtc tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac
    12241 ggtacagatg gtcagggctt tcgcgccaaa ttctgcagcg acgccgtaga taccagccgc
    12301 ttccatttcc acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg
    12361 agagtagaac aggtcagcgg agaacaggtt accaacgcga gcatcaatgc ccagtgcttt
    12421 agccgcgtct accgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatgatc
    12481 tttaaaacgg atgcggttaa ctttggaatc ggtgcaggca cccataccga taacgacgtc
    12541 gcgcagtttt acgtgcggca gaactgcgcc acaggaaccc acgcggataa ttttcttcac
    12601 gccgaaatcg gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg
    12661 acccattacg gaaattttgc ggcctttgta agtaccggtg aagcccagca taccgcgaac
    12721 gttgttcact tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg
    12781 gtcgcctggc atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt
    12841 agccattgtt ttatccttat caacatgttt tcaccctctc cctgagggag agggccgggg
    12901 tgagggcatc aagccgcacc cattatcaga acatggcttt gccatattcc atatcagaag
    12961 taccaaaata ttttgccaga gtctggccga tatccgcgaa ggtttcacga tgacccagtg
    13021 agcccggttt tactttcggg ccgtatacca gtaccggaat gtgttcacgc gtgtggtcag
    13081 taccggtcca ggtcggatcg caaccgtggt cagcggtgag gatcaggatg tcgtcatcgc
    13141 gcagcagaga catcagctcc ggcagacggc ggtcgaacag ttccagaccc gcggcataac
    13201 cggcgacgtc gcgacggtgg ccccaggaag agtcgaagtc aacgaagttg gtgaagacga
    13261 tggtgttatc acccgcttct ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc
    13321 cagtcgcttt cacttttttg gtgataccgc agttggcgta gatgtctgca attttaccga
    13381 cagaaaccac ctggccgtgt ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg
    13441 gctcaacagc caggtcgtga cggttaccgg tacgctggaa gttaccggct ttgtcaccga
    13501 taaacggacg agcgataaca cgtccgatgt tgtagccgcc gttggtcagc tcttcacggg
    13561 cgatttcgca cagttcgtag agtttatcca ggccaaaggt ttcttcatgg caggcaatct
    13621 ggaacacgga gtcagcggag gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc
    13681 ccagttgatc cagaatgacc gtaccggaag agtggcagtt accgaggtaa cccggcagat
    13741 tagcgcgttc gaccagttta tccagcagct cttgcgggaa gctgttttcg tgatcggaga
    13801 aatatcccca ctcaaacaga accgggacac cggcgatttc ccagtgacca gacggggtat
    13861 ctttaccgga tgacatttcg tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt
    13921 ccattcccgc cggaatgaaa ccggtagaac cttcgtgtgc tttcgccagc cccagacggg
    13981 tcagatttgg cagattgagc gggcctttac gaccgttatc agcttcgcct ttggcacaag
    14041 cttctgcgat atgacccagg gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg
    14101 tagcgccgat gccgaatgag tccagcacca taataaatgc acgtttcata tgttctccgt
    14161 actcagtgct tcaaatttaa atgatcagat cagtataccg ttattcgctg atacggcgat
    14221 agacagttgg tgtgctttcc ggtgctttat cggcaagttt aattgccgct ttcaccgctt
    14281 tcgccgcttc ctgccagctg ttttcgtctt tcgcgtggat aaccgccagc ggacgctgac
    14341 cgtctacctg gtcgcccaga cgtgccatat cagtaaagcc gacgctgtaa tcgatggtgt
    14401 cagatgcctg acggcgtccg ccgcccattg caaccactgc catccccagc gcgcgggtat
    14461 ccatttcact gacaaaacct tcggtatcag catagactgc tttcgtcagc atcgctgtcg
    14521 gcagatactt cgcgtagttc tcaacgaagt cggttgggcc tttttgtgcc gctaccatac
    14581 gaccaaagac ttctgccgct ttaccgttgt ccagcaccgc ctgcaatttc gcgcgcgctt
    14641 cggcgtcatc tttcgccagt ttgccggaga tcagcatctc cacgcacagc gccatcgtga
    14701 catcaaacag acgcgggtta cgatattcac ccgtcaggaa ctgcaccgct tcacgaactt
    14761 caaccgcgtt acctgcactg gaggccagta cctgattcat atcggtgagc agcgcggtgg
    14821 tgcgcacgcc agcgccgtta gccacgccaa caatcgcttc ggcaagggct tcagagagtt
    14881 cgtaggtcgg cataaacgcg ccgctaccca ctttcacgtc catcaccagc gcatccagac
    14941 cttccgccag tttcttcgcc aggatcgagg cagtgatcag cgggatggag tccacggttg
    15001 cggtaatatc acgggtcgcg tagaaacgtt tatccgccgg agccagtgag ctggtctggc
    15061 cgataatcgc tacgccgacg tctttaataa tttcgcggaa acggttgtca tccgggaaaa
    15121 tgtcgaagcc agggatggat tccagtttgt cgagcgtacc gccagtatga ccgaggccgc
    15181 gaccggagat catcggaata tagccgccgc aggctgcgac catcggcccc aacatcagcg
    15241 aagtcacatc gccgacgccg ccggtggagt gtttatcaac aatcgggcca ttcagatgca
    15301 ggcttttcca gtcgagaacg gttcctgaat ctcgcatcgc catggtcagc gagacacgct
    15361 caggcattgt catatcgtgg aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg
    15421 agatagtgtt gtcgcgaata ccgttgataa agaaacgaat ttcttcatcg ctcagcgcat
    15481 gaccatcacg ttttttacga ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa
    15541 aagagtatga ggcggctttt gccggatgcg tctacgcctt atccggccta cgggtagata
    15601 cgtttcgtag gcctgataag cgcagcgcat caggcgtaaa gcatcttact tagtagctgc
    15661 tggcgctctt accgtcaccg tggcccagcg ctttcaacag gcttgccagc aggctggaag
    15721 caccaaagcg gtagtgacgc gcatctgccc agtcagcacc gaacagttca tctgcgatgg
    15781 cgagatattt ctgcgcatct tccgcagtac gcacgccgcc cgctggtttg aaaccaacgg
    15841 ttttttctac gcccatatca cggatcactt ccatcatgat gcgtgcgctt tccggcgtcg
    15901 cgttcacagc cactttaccg gtagaggttt tgatgaagtc cgcacccgct ttgatggaga
    15961 tttcagacgc tttacggatc agcgcttcgt ctttcagttc gccggtttcg atgatcactt
    16021 tcagcagtac attcgctgcc gcgcaagcct ctttacaggc tttcaccagg tcaaaaccaa
    16081 cctgctcgtt acccgccatc agcgcgcggt acgggaacac aacgtcaact tcatcagcac
    16141 cgtaggcgat tgccgcacgg gtttctgcca gcgcgatttc gatgtcatcg ttaccgtgtg
    16201 ggaagttggt taccgtagcg attcggattt ccggggtgcc ctgctctttc agcgttttgc
    16261 gagcaatcgg gataaagcga ggatagatac agatagcggc ggtattgccg accggagttt
    16321 tggcctgatg acacagagca attactttct cgtcggtgtc gtcgtcattc agggtggtca
    16381 ggtccatcaa tttcagtgca cgcaggctgc ttgctttcag atcagtcatt tcattctcct
    16441 ggcttgttgc caataaaatt caccttgcga gtttgttagt attctaacat ctactccgca
    16501 acacacttcg atatacatca caattaagga aatctactta caagtttgca tcactgtaat
    16561 gcgatctggt tcaaataatt cactttcaaa tgaatgcgtc agtggtggca aacgcatcag
    16621 gatcaaagtg aacatcacga aacttcttac aatggcgcat caccttcggc ataaaaggaa
    16681 aagaagatgc ccacttctca tgaaaatgca ctgcaacaac gttgccagca aattgtcacc
    16741 agcccagtgc ttagcccgga gcagaagcgc cattttctgg ctctggaagc agaaaacaat
    16801 ctgccttatc cgcagcttcc tgccgaagcc cgccgcgcgc tggatgaggg tgtaatctgc
    16861 gatatgtttg aaggtcatgc gccgtacaaa ccgcgctatg tcttacccga ttacgcccgt
    16921 tttctggcga acggttccga atggctggag ctggaaggcg cgaaagatct tgatgacgca
    16981 ctctctctgc tgaccattct ttaccaccac gtaccgtcgg tcacatcgat gccggtctac
    17041 ctggggcaac tggatgcgtt gttgcaaccg tatgttagaa ttctaacaca agacgagatc
    17101 gatgttcgaa taaaacgttt ctggcgttac ctcgacagaa ccctgccaga cgcctttatg
    17161 cacgccaata tcggcccgtc tgattcgccc attacccgcg cgatcttacg tgcagacgca
    17221 gagttgaagc aggtttcacc gaacctgacc tttatctacg atcctgaaat cacccctgat
    17281 gacctgctgc tggaagtggc gaagaacatc tgtgaatgta gcaaaccgca catcgccaac
    17341 ggtccggtgc atgataaaat tttcacaaaa gggggctacg ggattgtgag ctgttacaac
    17401 tcactgccgc tggcgggtgg tggcagcacg ctggtacgcc ttaacctgaa agccattgcc
    17461 gagcgcagcg aatcgctgga tgacttcttt acgcgcactc taccgcacta ttgccagcag
    17521 cagatcgcca tcatcgatgc gcggtgtgaa ttcctctatc aacaatcaca cttctttgag
    17581 aatagcttcc tggtgaaaga agggctgatt aaccctgaac gttttgtgcc aatgtttggc
    17641 atgtatgggc tggcggaagc ggttaacttg ctgtgtgaga aagaagggat tgccgcacgt
    17701 tacggtaaag aagccgccgc aaatgaagtg ggttatcgca tcagcgcgca actggcggag
    17761 tttgtcgcca atacccccgt gaaatatgga tggcaaaaac gcgccatgtt acacgcacag
    17821 tcggggatca gttccgatat cggcaccacg ccgggcgcgc gtttacccta tggcgatgag
    17881 ccagatccga tcacccatct gcaaactgtc gccccgcatc atgcttatta ttattccggc
    17941 atcagcgaca ttctgacgct cgacgaaacc atcaaacgta atccgcaggc gctggtacag
    18001 ctttgcctcg gtgcctttaa agccggaatg cgtgaattta ccgccaatgt cagcggtaac
    18061 gatctggttc gcgttaccgg ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc
    18121 gaaggttcac gcaccaacac cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt
    18181 ctggaacgcc agccgcgcgt gataagccat gaacagcaga tgcgctttag tcagtaagat
    18241 tatccccttc tcctgcgttg acgggccagg cagtcgtctg gctctgtttt tgcagggctg
    18301 caatctgcgc tgcaaaaact gtcacaatcc gtggacgatg gggcgctgta atcattgcgg
    18361 agagtgcgtg ccacagtgtc cgcatcaggc gttgcagatt gttgacggca aagtggtgtg
    18421 gaacgctgcg gtctgcgagc agtgtgatac ctgcctgaag aggtgtccgc aacatgccac
    18481 gccgatggcg caatccatga gcgtggacga agtgcttagc catgtccgca aagcgg
//
LOCUS       DACAPB010000082        18459 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.082, whole genome shotgun sequence.
ACCESSION   DACAPB010000082 DACAPB010000000
VERSION     DACAPB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.082"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..889)
                     /locus_tag="IT904_002534"
     CDS             complement(<1..889)
                     /locus_tag="IT904_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3192301.1"
                     /translation="MTVKYYAILTNKGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVSNTEAITLKIDPSVVLATRKYVDDKISEHE
                     QSRRHPDASLTAKGFTQLSSATNSTSETLAATPRAVKVAYDLANGKYTAQDATTARKG
                     LVQLSSATNSDSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSTSET
                     QAATPKAVKAVYDLANGKQP"
     gene            complement(886..1497)
                     /locus_tag="IT904_002535"
     CDS             complement(886..1497)
                     /locus_tag="IT904_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP3192302.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTVGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYLELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGTGCYHGDALTVYPYTPEAIIVGGDYFPASVIHLIDNLRVNA"
     gene            complement(1490..2398)
                     /locus_tag="IT904_002536"
     CDS             complement(1490..2398)
                     /locus_tag="IT904_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP3192303.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNDEDVRPVADRVTVQSAAIVDYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNNTQASFCTEYSVVTGGSDE"
     gene            complement(2403..2750)
                     /locus_tag="IT904_002537"
     CDS             complement(2403..2750)
                     /locus_tag="IT904_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP3192304.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQSLSLTIPVS"
     gene            complement(2747..3382)
                     /locus_tag="IT904_002538"
     CDS             complement(2747..3382)
                     /locus_tag="IT904_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP3192305.1"
                     /translation="MNTLANIQELARALRNMIRTGIVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALYI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLTTGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3449..3901)
                     /locus_tag="IT904_002539"
     CDS             complement(3449..3901)
                     /locus_tag="IT904_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP3192306.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(3894..4361)
                     /locus_tag="IT904_002540"
     CDS             complement(3894..4361)
                     /locus_tag="IT904_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3192307.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVGGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4469..4894)
                     /locus_tag="IT904_002541"
     CDS             complement(4469..4894)
                     /locus_tag="IT904_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HAP3192308.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLHASLDRANNVASGQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACTDASDCPQRLPESEPLPDAGQ"
     gene            complement(4882..5307)
                     /locus_tag="IT904_002542"
     CDS             complement(4882..5307)
                     /locus_tag="IT904_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HAP3192309.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESMAVKIVTSLFLQPVTWCRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            complement(5322..5819)
                     /locus_tag="IT904_002543"
     CDS             complement(5322..5819)
                     /locus_tag="IT904_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HAP3192310.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(5819..6100)
                     /locus_tag="IT904_002544"
     CDS             complement(5819..6100)
                     /locus_tag="IT904_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP3192311.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKQ"
     gene            complement(6104..6307)
                     /locus_tag="IT904_002545"
     CDS             complement(6104..6307)
                     /locus_tag="IT904_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3192312.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6307..6816)
                     /locus_tag="IT904_002546"
     CDS             complement(6307..6816)
                     /locus_tag="IT904_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAP3192313.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(6916..7659)
                     /locus_tag="IT904_002547"
     CDS             complement(6916..7659)
                     /locus_tag="IT904_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HAP3192314.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPYWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSATPAKRGRPKKKAS"
     gene            complement(7663..8736)
                     /locus_tag="IT904_002548"
     CDS             complement(7663..8736)
                     /locus_tag="IT904_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HAP3192315.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQALDLIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(8795..9649)
                     /locus_tag="IT904_002549"
     CDS             complement(8795..9649)
                     /locus_tag="IT904_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAP3192316.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALLTNC"
     gene            9823..11595
                     /locus_tag="IT904_002550"
     CDS             9823..11595
                     /locus_tag="IT904_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HAP3192317.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11595..12629
                     /locus_tag="IT904_002551"
     CDS             11595..12629
                     /locus_tag="IT904_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP3192318.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(12964..14778)
                     /locus_tag="IT904_002552"
     CDS             complement(12964..14778)
                     /locus_tag="IT904_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP3192319.1"
                     /translation="MSHFKLSELSAIGYVVGLEGEKIRINLHEGLQGRLASHRDGVSS
                     VTQPGDLIGFDAGNILVVARVTDMAFVEADKAHKANIGTSDITDMPLRQIIAYAIGFI
                     KRNNDGCIFVSEDWRLPALGASAVPLTSNFLNTIYSIDEDELEKAIELGLDSRTKSVK
                     ILASIDKLLTRHMAVLGSTGYGKSNFNALLTRRISEKYPNSRIVIFDINGEYSKAFEG
                     IANVKHTILGMSPEGIPPRPPFVRGQVFSEKHVYYKQIPYQALGFAGLIKLLRPSDKT
                     QLPALRNALNAINRTHFEGSNFYLEDSKGTQFRIYDDCREEGQFSLGLWLDGLRNRTL
                     AKTDRWPPFKSIASLVAEFGCVAADFRSGGSKRDAFSYGNVLPLVKIIQQLSEDIRFK
                     NVVDMKGGHTLTNNGKHWESAMKNEVDYFFGREKGEENDWNVHIINLKNLSQDHAPML
                     LSALLEMFSEVLFLRGQDKSFPTVLLLEEAHHYLRDPYSEIDSQIKAYERLAKEGRKF
                     KCSLIVSTQRPSELSTTVLAMCSNWFALRLTNERDLQTLRYAMESGNELMIRQVSGLP
                     RGDAIAFGAAFNIPVRISIHQAIPGPRSSDAVYSEEWK"
     gene            complement(14765..16012)
                     /locus_tag="IT904_002553"
     CDS             complement(14765..16012)
                     /locus_tag="IT904_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192320.1"
                     /translation="MIIYQGGKKICEDDFRSHVYSLCQLDNVGVLLGAGASVGCGGNT
                     MKQVWQSFKQEYPKLLALLIEKYFLVTQADSDNDIVNVELLIDEATKFLSVAKTRHNE
                     VEVKEVGLILSSLYKEVTKAALLTGSDFRNKNQGKKSQFRDHKELISKLISNRQPGQS
                     APALFTTNYDLALEWSAEDLGIQLFNGFAGLHTRQFYPQNFDLAFRNVNAKGEARFGH
                     YHAYLYKLHGSLTWYQDDCLTVSEISASQAYDNYIQNIINEDGYFHGQHLIYPGANKY
                     SHTIGFVYGEMFRRFGEFLSKPQTALFVNGFGFGDYHINRIILGALLNPSFHIVIYYP
                     ELDSAQSKVEKNLASESEKAIINLKNLTFNQVTIVGGGEYAYFDSFVKHLPYPVLFPR
                     ENTVDDLVEAISKLSKGDGNVSF"
     gene            complement(16215..18335)
                     /locus_tag="IT904_002554"
     CDS             complement(16215..18335)
                     /locus_tag="IT904_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HAP3192321.1"
                     /translation="MTVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTATFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSFDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCHIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGMLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDQDVVRVLRGALKYSLSTRKHH
                     HSNYNLLKPHKITSSARLTKSCLS"
BASE COUNT         4333 a         5147 c         4559 g         4420 t
ORIGIN      
        1 caggttgttt tccattggca aggtcatata cagccttcac ggcttttggt gtggcagcct
       61 gcgtttctga cgtgctgtta gtggcgctgc tgagctggac aaggcctttt cgcgctgtgg
      121 tcgcgtcctg tgcggtatat ttcccgttag caaggtcata tgctgtcttt accgcctttg
      181 gcgttgccgc aagcgtttca gaatcgctgt tggtggcgct actgagctgg acaagacctt
      241 ttcgcgctgt ggtcgcgtcc tgtgcagtat atttcccgtt agcaaggtca tacgcgacct
      301 ttaccgctct cggcgttgcg gccagtgttt cagacgtgct gttggttgcg ctgcttaact
      361 gagtaaaacc ttttgcggtc agcgatgcat ccgggtgtcg tcgtgactgt tcgtgttctg
      421 agattttgtc atccacatat ttacgggtcg ccagtaccac cgacgggtcg attttcagcg
      481 tgatggcttc ggtattcgag acaaccagaa tcatgcggat agtctgggta cgaccactgc
      541 cttcctgcaa ctgcggtttg tacgtttccg ggcagttcgc caccgcaatg agtacgcctt
      601 catcatcata aagcccaatc tcacggatcc agaatcctcc ctcgttttca gggatgattt
      661 gctccgcaat aatctggctc tgattgttag ggtcaacact cagaagattc agcggtgcaa
      721 tgcgtttctg gttaatcagt tttgtctgtg ccgggtctgg tgtcggtaat acaccattcg
      781 catcaccaac ggccatttgc gtcagattca gcttactgcc gagcatcgtc gcgttagcca
      841 gtcgtgctgc gcccttatta gtcagaatgg cgtagtattt cactgtcatg cgtttactct
      901 caggttatca attaaatgaa tgaccgaggc cgggaaataa tcccctccga caataatggc
      961 ctccggggtg tagggataaa ccgtcagggc gtcaccgtga tagcatcccg ttccggcaaa
     1021 aatgttgccg gttgtactta aactgatagc cagtcccgtc agatggcggc tcgccggttt
     1081 tgcatcagca acgagacgtt ccagctccag atacatttct tcggtaatac cctgctcaag
     1141 cacgccaaca acgatacgga acgtccccgg ctcctcgttg agctgccacc actccctcac
     1201 ttcaatcaga tagccgagcg gctccacaac acgccggatt gcgccgacag tacccttatg
     1261 acagtggata aaataggcat cgcggataac ggcgcgtttt gtcgcttccg gccacttttc
     1321 atcccacctg tcgaccgaaa aggcccacgc cagccacggc agcagatttg ccgggcaggt
     1381 gtccgggttc cacagctcac gaatactgac cggcgttttt tcaatttccg cacaggcttt
     1441 tgcggcggcg acttcaagcg gtgatgagcc ggtcggcagc agtcgcgaat cactcatccg
     1501 agcctcctgt cacgacgctg tattcggtac agaaagacgc ctgcgtattg ttgagcacga
     1561 tgtcggccag cggtgcagcc agttcgacac gctgcacgcc ttccacatgc aaagcggcat
     1621 aaatggcaga cagacggatg tcgcgcccca gccggtgctg tgccgtgata tacgcttcca
     1681 gttttttcac ggcggcagcg cgaatgggtt cgctttcggg accagggtaa aggtaaagcg
     1741 tggcgtttat ctggtaatca acgatggcag cagactgcac ggtcacgcgg tcggccaccg
     1801 gcctgacgtc ctcgtcatta agggcgttac gtaccacagc cagcaggtct tcggatgcga
     1861 caccgttatt ttcacgtgac agcacagaga tagtgacaca ggccggagac ggactggtga
     1921 cagagatatc cgcgacacgc ccgtcggcac tgcgaccatg atactgatag gcccccaccg
     1981 acccggcgac gcttaaacct tcaaaggcct gctgaatacg cagacgataa tcggtgtcag
     2041 attccatcac tgccggtgtc ggcgggatgg tcgaatcatc tgccggggtg atagtcaggc
     2101 gcgtggtgtt gtaattggca ccaatcacat caaggtcatt accggcggca caggccagca
     2161 tcaccgcccg tgcggcctca ttcacacgct gacgccagat aagctcacga taagcatttt
     2221 cctccagcag tttgacgaga ggctcggatt ccagcgtcag ggtacgggcg accgcctcct
     2281 gctggtcttc cgggtaaagg gaaatcagtg tcgccttgcg ttcagcgaga atggtttcaa
     2341 agtccagctc ctcgaccaca tccggtgcgg gtagctggtt caggtcgata atcggcatgg
     2401 tttcaactca cagggatggt taacgaaagt gactggccgg tgtcgttgtg ctggccggtt
     2461 aacgtgaccg tcattcgccc gtcaaaactg cgcgccgtgg tgacggatga cagggtgacg
     2521 cggggttccc atttcagcac tgccatgtaa caggcgacct taatctgcaa ctcaagcgcc
     2581 ggagtctgcg gctggtcaat cattgacgcc agcaacgagc cgtaatcacg acgcatcacc
     2641 cgtgagccga ccggtgtgcg caggatatcg ccgatactct ggctgatatg ctcaaggtca
     2701 gtgaccgtca ggccatcact gcgattcatt ccgagataac gcgctgtcat aaaggactcc
     2761 cggttgtgcc gccgctgtcg ccggggtgtt tgtgggtatg cagtacctta ccgtttgatg
     2821 agagttcacc gccggtgtgt tcaatgttgc cgcgcatcgt cccgcccttc tgcacttcca
     2881 gcgtgccggt agtcagtttg ttagtgcaga ccacttccgg tgtgtccagg gtgacacggg
     2941 ttgacgcttt caccgtgacc accggtaccg tggcagtaac agaatcagaa gccgtcacgc
     3001 tggccgtttt aattccgctt gccgtgagtg cactggtttc gggttcatac tcaatcaccg
     3061 ccccgtcagg gaaacggata tacagggcat ccgccgacgc agacggcgca gggttatcgc
     3121 cggaataaat ccccggcaga acgaacgccg tgtcgagttc accgcccacg gccagaatca
     3181 gcacctgttc ccccacggaa ggtgcccacc atgtgcgcga acgtccggcg cgatgggtca
     3241 gccactgaag ccagtcggtg cacatgccgc cggtctgcac tcggcagcga ccggcgttaa
     3301 ggtcggtttc gacgacaatg ccggtgcgta tcatgttgcg cagtgcgcgc gcgagttcct
     3361 gaatatttgc gagagtgttc ataacgggaa ggatgccgcc gggtcatacc ggcggcaatg
     3421 tgacgatgag gtgtcgggaa tggcacaact aacggtcgag gtgagccagg ataatctctt
     3481 caatcatctg cacatcctca ccggtaaagc caagcagagg acgcgccgga taatcaattt
     3541 tcttaccgtc tttccgggtt tcttccgaca gaccgaactg atgcacactg gcgattttcg
     3601 gcgacttccc gccgtaaaat tccattgatg cctgctccgg gctggcgcgg atatgcaaaa
     3661 aacgactggt gataagtttc gcaaacattt ttcgcttaac acgaccggtc ttttttctga
     3721 cgctctgctg ctgacgtggc gcgtagggtg tgccgtccgg ggctttctgc gccatcaccc
     3781 gacgctgctg actctgccgc agacgtttcg ccagttcggc actcagtcgc cgacgccctg
     3841 acggtgacag cgactcaatc agtccggtca gccggtcttc aaaacgctta aactcattca
     3901 tcccacttgc tcaccagttc gccattgata tacagctcca tcgggcgggt gaccggctcc
     3961 ggcggcggag gttccgggat gttcttcaca tgcagtgcgc cgcccacctc actgaccagc
     4021 gtgcgctcgg tcagcatcag gctgatgctg atatcaaagc tgctgtcatt gttgatgtct
     4081 gcataaaacg tgaagccctt tttctggcct tcgtcggtgg tcatgatgtc gggctgattt
     4141 tcccgcagcc acgccagcac cggcacgatg agcaggtcaa aatcaccggt aaagtcggtc
     4201 acaatcacat tgagcgtgta acgcttttcg aacgacagcg acgtcgccag cgtggaggca
     4261 atactcccgt tatccacgaa tatccgcagc atatcggggt tagttttcag caccgtgacg
     4321 gcatcagtca gcgccctgcg caggctgtcg ggtttgagca tcgttttcgt cctgacagtg
     4381 tttaatcatt tttacctggc tggcacagcg tgccagcgcg ttctcaagct gccggatatc
     4441 ggcacttaaa tcaccgttcg tctccgggtc actgcccggc atcgggcaaa ggctcacttt
     4501 cgggcaggcg ttgtggacaa tcactggcgt cggtgcaggc ggggcgctgg tgcaaccggc
     4561 gcacagcatc aggcaggtca gcaccgtacc agcggcgaaa atcttcgttc tcattaagta
     4621 acctcgtgat ggttttctcg cgctgtgctt cacgcttcgc ggcgttctcc agttcctgac
     4681 gcagtgccac ctgcgccagc tcgtttttat ctgccctggt gagcgcaaca tgaagctgat
     4741 ttttcagcat ggtgatggtc gtctgctgac cgctggcgac gttgttcgcc ctgtccagcg
     4801 aggcgtgcag gctggcgttt tcatgcttcg ccagaaacag ccccgccacc gccagtgata
     4861 acaacacaac cagcacaatc atcagctttg acatggttcc cgcccctcaa aacgctgacg
     4921 gcaggccgta cgtatcagcc ggaagaacac cgacgccacg agataaatca gcgcagtaaa
     4981 aatccacccg gcggcgacca gcgagataaa tgtcgccacc atcaccacca gagccgccgc
     5041 ccgtctgcac cacgtcaccg gctgcaaaaa cagcgacgtg acaatcttca cagccatcga
     5101 ttccggcggc agctcccgcc cgtagcgttc cagcacatac tccgtggcat acacgccgac
     5161 accaccggca accacacaga taaccgtcgc cagaatcgcc caggcggcga caaaactgac
     5221 ggccacgctc tgcgggtaaa tcagggacag tgccagcatc agcgccagcg acacgttcag
     5281 catcagtgaa agggataatt tcttcatggt gtttactccg tttaagccgg tacgccgcca
     5341 gcggtacgcc agacggtgac cagtttttcc agtgaatgct cacgctgacc gtaaccggca
     5401 cccggcaggg acgcccagat attgcgacag cgtgaaatgg cgcgctcaat acgtcccgcc
     5461 cggatgtcat ccagtgcacc gcgttcgcgg atcaactgaa tggcgagtct gtcctgtgac
     5521 aacggactga aatccggcag ggcaagctgt ttgcggtaat gcggccagaa caggtaaagc
     5581 tgctgatagc gaccggaggc cgtggatttt tcaccgcgac ggttaaacac cttcgccggt
     5641 cggccatgcg cgaacgggtg gtcactgtag tcggtgaaaa tttccggctt tccgtccagt
     5701 ccggtgacta tcacgtcata gccccggttt ttcgtcagcg gatgattcgc cgtcccttcg
     5761 gacactgcca gcatgtcgag aaaggcggcg atattctgat gcgtgttaat taccggcatt
     5821 actgtttccc cctgccctta aagcggcgct gaatggcaat ctcaatcacc tgataaccgg
     5881 cgatacccag catggagccg ataccgcaca ccgcaggcag tgacaggtca ggaaactgca
     5941 ccagaacaac accggcaacc atcgagacaa aaccaccgag caacatgcgc ccgataaaca
     6001 gacgcggggt gatgggttca ccaccggcaa gcaccttgcc gacaacaatc agcaccccaa
     6061 tcatgaaaag cgacaggacg cttttttctt ctgctgtcat gcgttactcc cacagattga
     6121 cagtttcagc cacgggcgcg gtctgaacgt cgggcagttc gacggcggtg ccgtgtggca
     6181 gcaccgcacc cagttcagcc agtcccggat ttgcggcgag cacggtctca accacgccct
     6241 cagtgcgccc gtaataccgg acacagatgg cgtcgagcgt gtcgccctgt agcgcaaagg
     6301 tcttcatcag atttgactca cgatgcagcg cggcttgtcc tggatgcgcg ccactgccca
     6361 gcgcatatcc cgccacagct catcaatggt gctgtcaatg ctgtcggcct tcttgtcgcc
     6421 tttcgcactg gcatccacgc cgcgataacg ctcataaagc gatgcggtcg ccatcgcaca
     6481 cacggcgcgc tcgtagtaaa aaactttgat gctttcaccg tcgatgtcgt ccgccgggac
     6541 gtccgccaga cgcgtaaaac cggcagcaat tttctgttcg cggtactcgt acagctccgc
     6601 attcgtctcc gccatgcctg acttgatggc ctcacgcaga cgggcggggg cgacggtctg
     6661 ctcaaggcgc atacgttccc ggacgcgctt cgggtcgata tcgggaaaaa agaacgtgtt
     6721 tttaatcacc ggctcgtcgc ctgccggttg cgggatgacc accgtaccct caccggacac
     6781 gggagcctcc tttcgcggaa taatcagcgt catcatgact acctctgaaa agtcgggcgg
     6841 tggacgccgg tacagcgtca ggtgattcac cctcactgac cggcgtgccg ccctggcgcg
     6901 gggcgcattc ggttgttaac tggctttctt tttcgggcgt ccacgttttg ccggtgtcgc
     6961 actccgggtc ttacgcgggg cgcgggtggc cgcttttggc tgcggctccg gcttcggttt
     7021 cagctcccgc tccagtcgtt caatctcttt tttgacgcct gcctgacagt cgagctgtgt
     7081 cgcgcgttgc aggtgagcca gcgcccctgc ggcatcacca gcgtcacgca gaaacagacc
     7141 ggtgattttg tgcagctttg cgcgcacttc atcaggcatg tcagccgtgg cggtcagttc
     7201 gagggtgtcc gtcagcaggc gggtatccac ggattcaccg gcagcgtggg cacgcatggc
     7261 cgcgagtgcg acctcctcgg tgaacatgta cggcggggta cggcggtgtt tacccggcat
     7321 ggtcagaccg tatttcaggg cataacgggc aatctccagc gcaccggcaa tatcgccggt
     7381 atccagacgc cacagcatga ccgtcatcag aatgtcatcc tgtgcacctt tgccctgttc
     7441 cagcacgccg ttcacccacg gcaaccagta cggcagcagt tcgcgttttt ttgcggcctt
     7501 cagctctttt gaataaatcg ctttcagtgt gcgctggtct gcggcgagct taaccagcat
     7561 ctgctcatag acagttgcat gtcgcagcgg ggcggcttcc cgctgcgcgg tcatcgctgc
     7621 cgagacccgc atcatgtggc gctgtgcggg actcgtcatc ggttacgctc ccggctctgc
     7681 ggtcgcctta gccggtgtgg agaaatcacc gaccttaatt ttttccacca gacaaccggc
     7741 ggcgtagtct tccaccacgt aatcaatgtt cattgactcg tagttctcca cgcggtcgag
     7801 tttcgggttt tcctcaatca cgcggcgatg gctgtcatcc atgtagtaga tggacaggtt
     7861 ttccagcttc gtgatgagca tcgcatccgc cgggaagtac gggacgcgta ccgccggcag
     7921 gttaccgatg cgtttctggc tgatgatgac gtcagcggcc agcatttcgc tgttgtcctg
     7981 ctccttgttg acgataggga aatacttgtc cgccagtagc tgacgcccca caatcaccac
     8041 aaggtcaggg tcttcctgat accacggctc aatcaggttg ttggtcgcat ccattaccag
     8101 tgcatcgagg ctggcataat caccgccctt acccacgcgg atgacctcag aggtggtgtg
     8161 accttcctcg tcagtaacct tgctcatcac gcgcgccggg gcttcattgc ggtatttctg
     8221 cagccagccg accgccacat cctgcagcat cgggttactg ctgcggtcag aggtttcggc
     8281 acgcttcaca ccgttaaaac cggccatgat taaatcaaga gcctgacgtt tgataatggc
     8341 gttacggaca cggagctgga aatcctgata acgcgcccac aggtccagcg ttttgtagcg
     8401 gatataaaaa tcgaagttaa tctggtcgca ttcgtacttg ttggacgcca gcttcgagaa
     8461 gtccttcggc tgacgctcgg tgccaccggc ggtgtcggtg gtgctggcaa tggagccggt
     8521 gacaccgata ccaatttttt cccctttcat ttcgctgacc ggcacaatgt tgatgcgggt
     8581 cagaaagtca gaggactcct gcatggtgtt catcagggtc tgggtgaccg acggttcaac
     8641 ggtgaatttt ttcgacacat caccggcgtc gatgccgttc agttcggcaa cacgggacag
     8701 gtaggcatta aatttaaagc gggtttcctg gcgcatagtt tttcctgaaa ttaagggtta
     8761 atcgtgaagg ttttcccgga ctgactgacg ccggtcagca gttcgtcagc agggcatcac
     8821 cgccaccgcc ggtggcttta ctgcggcgct gctgggtcag actttcggtg tggtcgagac
     8881 tgtttttcag gcgggtgaat gcctggctgg tttcatccgc cctgtcagtc acatcctgct
     8941 taagtgcgga aaaggcggtt tccatctcag cgaggcgctg ctcagtggcg ctcagttttt
     9001 cctgcacatg ttcagcaaca gcggtcaccg cttcatgcac gtcattcaga cgggcgtcat
     9061 cgctggcctg tttgcggcca aaaatggact tcaccttttc ggtcagggcg gtgaacacgg
     9121 tttcaggcag gtcttcaaat tccagctcaa caggcgttgc cactgaaatc aggttttcag
     9181 ggcttaattt gaagcggttc agggggttgt gttttgccgt gcggcagaat tccaggtatt
     9241 ccgtgccgag gcttgccggg tcatcggtga cggccagccc caccagataa catttgccgg
     9301 tgttggcaaa gttcggctga atttccattg aggtatagac cttctgcgcg gccttgttca
     9361 tcgcgataag gtcatcggtc ggggtgattt tcgcaaacag cgcccatttg cctttcagcg
     9421 ccgaatcatc gtcaatcttt tcggccttca gttcggccac atcgccgtaa cgcttaaaaa
     9481 taccgtcagg caggatgccg cgcagatgtt ccaggttaat gcggcaacca tagactcgcg
     9541 ggtcaaaggt ttcggccatt tcctgaatat cctgcgcact gatgacacgc ccgtcacagg
     9601 tgtcaccctc aacgccgata cgaaagaatt ttgagacttt ttttgccatt gtcaggagtc
     9661 ctgaatagtg attagaggag tcacatgtcg gcatcagttt cccgacgatg cgcatcctcc
     9721 gccatcagtc ccggatggct tatcaccgac acaacagcac cttagcgaat cgcggggcgc
     9781 gactcagtag ccttgccgtg tattcatcac ggcgaggtat tcatgaccat caccacagac
     9841 accactcttt tacacgaccc gcgtcgtcag gcggcgctgc tgtactggca ggggttttcc
     9901 gtgccgcaga ttgccgccat gttgcagatg aaacgcccga cggtgcagag ctggaaacag
     9961 cgcgacggct gggacagcgt tgcccccatc agccgtgtcg aaatgagtct ggaagcgcgg
    10021 ctgacccagc tcatcatcaa accgcagaaa accggcggtg acttcaagga aattgacctg
    10081 ctcggacgcc agattgaacg actggcacgg gtcaaccgct acagtcagac cggcaacgaa
    10141 gcagacctta atccgaacgt tgctaaccgc aacaaaggcg ggcgtcgcaa accgaaaaag
    10201 aattttttca gtgacgaggc catcgaaaag ctggagcaga ttttctttga gcagtctttc
    10261 gactatcagt tgcactggta tcgtgccggg cttgagcacc gcatccgcga tatcctgaaa
    10321 tcccgccaga ttggcgcgac gttttatttt tcccgcgagg cgctgctgcg cgccctgaaa
    10381 accggccata accagatttt tctgtcggcc agtaaaacgc aggcgtatgt gttccgtgaa
    10441 tacatcatcg cctttgcccg tctggttgac gttgacctga ccggtgaccc gattgtcctg
    10501 ggcaataacg gcgcaaaact gatttttctc ggcaccaact ccaacaccgc acagagccat
    10561 aacggcgacc tgtacgtcga cgagattttc tggatcccga attttcaggt actgcgtaag
    10621 gtggcatcag gtatggcctc acagagtcac ctgcgctcga cctatttctc caccccgtcc
    10681 acgctggcgc acgacgccta cccgttctgg tccggtgaac tgttcaaccg gggacgcgcc
    10741 agcgccgccg aacgcgtgga aatcgacgtc agtcataacg cccttgccgg tgggcttctc
    10801 tgtgcggacg gccagtggcg acagattgtc accattgagg acgccctgaa aggcggctgc
    10861 acgctgttcg acattgagca gctcaaacgc gaaaacagcg ccgacgattt taaaaacctg
    10921 ttcatgtgtg aatttgttga cgacaaggcg tcggtgttcc cgttcgagga gctgcaacgc
    10981 tgcatggtcg acacgctgga agaatgggaa gactatgcac cctttgccgc caatccgttc
    11041 ggctcccgcc cggtatggat tggttacgac ccgtcacacc gtggcgacag tgccggatgc
    11101 gtggtgctgg caccgccggt ggtggccggt ggcaaattca gaatacttga gcgtcaccag
    11161 tggaaaggca tggactttgc cactcaggct gaatccatcc gcaaactcac cgaaaaatac
    11221 aacgtcgaat acatcggtat tgatgccacc ggcctcggtg tcggcgtgtt ccagctcgtg
    11281 cgctcgttct atcccgccgc gcgcgatatc cgctacacac cggaaatgaa aaccgcaatg
    11341 gtgctcaagg caaaagacgt tatccgccgt ggctgtctgg aatatgacgt cagcgccacc
    11401 gacatcacca gctcgtttat ggctatccgc aagaccatga ccagcagcgg acgcagcgcc
    11461 acctatgagg ccagccgcag cgaggaagcc agccacgccg acctcgcctg ggcgaccatg
    11521 catgccctgt taaatgagcc actcaccgcc ggtatcagca ccccgctgac atccaccatt
    11581 ctggagtttt actgatgagc aagaaaaaag ggaaaacacc gcaacctgcg gcaaaaaaaa
    11641 tgaccgccag cgccccgaaa atggaggcat tcacctttgg tgagccggtg ccggtgctcg
    11701 accgccgtga cattctggat tacgtcgaat gtatcagtaa cggcagatgg tatgagccgc
    11761 cggtcagctt taccggtctg gcaaaaagcc tgcgtgctgc cgtgcatcac agctcaccga
    11821 tttacgtcaa acgtaatatt ctggcttcaa cgtttatccc gcacccgtgg ctttcccagc
    11881 aggatttcag ccgctttgtg ctggattttc tggtgttcgg taatgcgttt ctggaaaagc
    11941 gttacagcac caccggcaag gtcatcagac tggaaacctc accggcaaaa tatacccgcc
    12001 gtggtgtgga agaggatgtt tactggtggg tgccgtcctt caacgagccg acagccttcg
    12061 cacccggctc cgtgtttcac ctgctggagc cggatattaa tcaggagttg tacggcctgc
    12121 cagaatatct cagcgccctt aactctgcct ggctgaatga gtcggccacg ctgttccgcc
    12181 gcaagtatta cgaaaatggc gcacatgccg gatacatcat gtacgtcacc gatgccgtgc
    12241 aggatcgcaa cgatatcgaa atgcttcgcg aaaacatggt taagtcgaaa ggccgcaaca
    12301 actttaaaaa tctgtttctc tatgccccac aggggaaagc cgacggcatt aaaattatcc
    12361 cgctcagtga agtcgcaacg aaggacgatt tttttaatat caaaaaagcc agcgccgctg
    12421 acctgctgga cgcgcaccgc atcccctttc agttgatggg aggcaagccg gagaacgtcg
    12481 ggtcgctggg cgatattgag aaagtggcaa aggtctttgt ccgcaatgag cttatcccgt
    12541 tacaggacag gattcgggaa ataaacggct ggctcggcca ggaggtcatc cgctttaaaa
    12601 actactcact ggacactgac aacggctgaa catcgccgcc tgcgggcggc ttttttacac
    12661 cccgtcatca cgccctcaca cgctcaccac cgcacaaaac accccgcaga cacaccaacg
    12721 ccccggcgca caatataaac gccatcacga cgcgctcaga cgctgaaaaa ataaaatcag
    12781 caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg gggcggtttt
    12841 aatgcagtta catgacctta taaaagccac ataactgctg ttctgaataa ggtattaaac
    12901 tcctgtaacg ctcattcaat ttaacgcaga ttgatgcaat tgctcactgc agtacaatcc
    12961 cgattatttc cattcttcag aatagactgc atctgacgat ctaggaccag gtatagcctg
    13021 atgaatagaa attctcactg gtatattaaa agctgcacca aacgcaattg catcccccct
    13081 aggaagacct gaaacttgtc taatcatcaa ttcattccca ctctccatcg catatcggag
    13141 agtttgcagg tctcgttcat tagttagccg caatgcaaac caatttgaac acatagctaa
    13201 tacagtagta gaaagttctg atggacgctg agtactaaca atcaatgaac atttaaattt
    13261 gcgtccttct tttgcaagac gttcataagc cttaatttga gaatcaatct cagagtaagg
    13321 atctctcaaa taatgatgtg cttcttcaag aagtaaaaca gttgggaaag acttatcttg
    13381 ccctcttaaa aaaagaactt cagaaaacat ttctaataaa gcactcaaca gcattggtgc
    13441 atggtcttgc gaaagatttt taagattaat aatatgcaca ttccaatcat tttcctctcc
    13501 tttctctcgg ccaaaaaaat agtcaacttc atttttcatt gccgattccc aatgtttacc
    13561 attattagtt aaagtatgcc ctcccttcat atctacaaca tttttgaatc tgatatcctc
    13621 tgatagctgc tgaataattt tcactaaagg aagcacattg ccataactaa aagcatctct
    13681 tttacttcca ccagaacgaa agtccgcagc aacacaaccg aactcggcta ctaaactagc
    13741 aatacttttg aatggaggcc atcgatctgt tttagcaagt gtcctatttc ttaacccatc
    13801 caaccataat ccaagcgaga attgcccttc ctctcggcaa tcatcatata tccgaaactg
    13861 agttccttta ctatcttcca gataaaaatt agaaccttca aaatgtgttc tatttatagc
    13921 attaagagca ttcctcaacg ctggcaactg tgttttatcg ctaggacgta gtaattttat
    13981 caaccctgca aaaccaagcg cttgatacgg tatttgctta taatagacat gtttttcaga
    14041 aaatacctga cctcgcacaa atggaggtcg cggtggtata ccttcaggac tcatccccaa
    14101 aatagtatgt ttaacattcg ctattccttc aaatgcttta gaatactcgc cattaatatc
    14161 aaaaatcaca attctcgaat taggatactt ctcggaaata cgcctggtca acagtgcatt
    14221 aaaatttgat tttccatacc cagtactccc taacacagcc atatgacgag tcaacaactt
    14281 atcaatactc gctaagattt ttactgattt agttcttgaa tccaaaccta actctatggc
    14341 tttttccaat tcatcctcat cgatactata aatcgtattt aaaaaattag atgttaacgg
    14401 aaccgctgag gcccccaatg ctggtagtct ccaatcctct gaaacaaaaa tacacccgtc
    14461 gttgtttctc ttaatgaaac caatggcata agctataatc tgccttaaag gcatatccgt
    14521 tatatccgac gtaccgatat ttgctttatg agctttatca gcctcgacaa acgccatatc
    14581 tgtaactcgg gcaacgacca aaatatttcc agcatcaaaa ccaatcaaat caccaggctg
    14641 agtaacagaa ctcacaccat ccctatgaga ggccaatctt ccttgtagac cttcatgaag
    14701 gttaatcctt attttctcac cttccaaacc aacaacataa ccaattgcag aaagctctga
    14761 gagtttaaaa tgagacattc ccatcccctt tagataattt agaaatagcc tcaaccagat
    14821 catcaacagt attttctctt ggaaagagaa caggataggg aagatgtttt acaaaactgt
    14881 caaagtaggc atattcacct ccaccaacaa tagtcacctg attaaatgtc aaatttttta
    14941 aattaattat tgccttttca ctttctgaag ccaagttttt ttcaactttt gattgagcac
    15001 tatctaactc tggataataa ataacgatat gaaatgatgg atttaacaac gcccctaaaa
    15061 ttattcgatt tatatggtaa tcaccgaatc caaaaccatt tacaaacaat gctgtttgtg
    15121 gctttgacaa aaactctcca aatcgtctaa acatctcgcc atacacaaaa ccaatagtat
    15181 ggctatattt atttgcaccg gggtaaatta agtgttgacc atggaaataa ccatcttcat
    15241 ttatgatatt ttgtatatag ttatcatagg cttgagaagc acttatttca ctaactgtta
    15301 aacaatcatc ctgataccaa gtaagagaac cgtgcaattt ataaagatag gcatgataat
    15361 gtccaaaacg tgcttctccc tttgcattta catttctaaa agccaaatcg aaattttgag
    15421 ggtaaaattg tcgcgtgtgt aatccagcaa atccattaaa caactgaatc cctaaatcct
    15481 ctgctgacca ttctaaagcc aaatcataat ttgttgtaaa aagtgcaggt gccgattgac
    15541 ctggttgacg atttgatatt agtttggata ttagctcttt atgatctcgg aattgagatt
    15601 tcttaccctg gtttttattt ctaaaatcac taccagtcaa taatgcagct tttgtcactt
    15661 ctttatataa actgcttaat atcaaaccaa cttccttaac ctcaacttca ttatgtcgag
    15721 tttttgcgac tgataaaaat ttagtagctt catcaatcaa taattccaca ttgactatat
    15781 cattatcaga atcagcctga gtgaccaaaa aatatttttc aattagcaat gccagaagct
    15841 tcggatactc ttgcttaaac gattgccaca cctgcttcat tgtgttccca ccacaaccaa
    15901 cagatgcgcc agctcccaag agaaccccaa cattatccaa ctggcataaa gagtaaacat
    15961 gagaacgaaa atcatcctca caaatctttt taccaccttg ataaataatc ataaacgccc
    16021 ctttcgacta aagtttgaat ctgaaaacaa cctaaattga aatgtagatt atgagagaat
    16081 ttttttagaa cgaaaagccc attcattcaa ggagttaaaa ccatcagaaa tcactgtttt
    16141 actacatgcc agaactcccc ttatggcttg tctgttacgc catgctgagc taggtctaca
    16201 caactacctg taatttacga cagacaggat ttagtcagcc tcgcagatga cgtaatttta
    16261 tgcggtttta acagattgta attactatgg tggtgctttc tcgtagacaa agagtatttt
    16321 aatgcccccc tgagcaccct cacgacatcc tggtcattcc attcgataac tccgtcatca
    16381 accagattaa gcactgctgc ggcgtgctca gaaggtgtgg gagccggtaa cgaagtatca
    16441 ccaccggtga gcattccaca gttattgaca ggactccgag gcgcggcgat gccgcttttt
    16501 aaagtcaaag gctcaacgac cggaactttc ggcacaatgc gccagtccgt cgttctggtg
    16561 atatgaatat gacgcgcgcc gagatgcggc gcgtaaatgc cgaccactct ctcgacttct
    16621 tcctcgtact cgttaacttc atccgacgga ctacgggcaa ccctgacagt ctgacaatcg
    16681 cgcgggacat ttgccccacc ctgcgcgctg atatacaacg caaaatcacc actgtctgcg
    16741 gcggcgcgtg cagcctcgac gcgctcgtca aactcatcag caatgctgac gccgcgcggc
    16801 aatttgcgta gttcacggta agcccccatt gtcggcagtc caaccgtttt aaattgcggg
    16861 atgcgccacg ttgacgccca tgcggtaaca gccgcggcag tatctttaag cggcttaccg
    16921 gtatcgttat cgagctgacc atccagtgca tagccgtcga tattttttga aatgtatttc
    16981 gcgatatatc ccgcagcacc gccccggtta aggtgctttg cctgaaaacg gtttcgcgcg
    17041 gctcctcttt cgtcgccatc ctctttgagc gcatagcgac gcatgatttc gataatctgg
    17101 ttacgctggc gcggattaca aaaaagcatc atatgccagt gcggcgttcc gtcgtggtgt
    17161 ggctcgacga cacgcaaacc gtagacctgt aaatcattat ccttgaatgc cgtgcgcatc
    17221 aggctccaga tatggcagag ataacgctgc gcatcctttg gattaaatgc ctcatcgttc
    17281 cagccgtgat ttagctggac ggttttactt tcgccttttc cgacctgacg tgtcgggtga
    17341 tactttgacg gcgcggtcag cgtgataaac atccccacat caccctctgc tgcggcgtaa
    17401 cgctcaatac cggcaatggt gttcatcagc tccatccggc gaatttcagg attagaaata
    17461 ctgcccatca ccttactgat aaggtcgatg cgctcgccgg tttccctgtt ttcaaggtca
    17521 aacgatttaa gaaattccag atttgcctgg cggcgtgcac gtacatcacg aatggcatgt
    17581 ttactggcat aaggagaacg gtctttattg acctccccga cagcaatcag taacgcctca
    17641 tgccagcgca tacgctggcc tttaagctga tgagtccacc actcatcgtt aaacaggcgg
    17701 gcaatggcag aatatgcctg cctcgtggtc atctgtcctt tacggtattt tttccagtag
    17761 agcggggaaa tattgaaagc acgtgcagcg ccagcaacat gaccatacag gtgagcctgc
    17821 gcctcatccg taaacagcga ttctttttcg ccatgggcat ccacccaggc atcgcagagt
    17881 tcctcataca tcatgaaaag ctgcgatgag atacgggcgg caaatttttt cagctccttg
    17941 tcattcattc ccggcaggcg cgcatagtgg tcacgctctg ccagaaacag taacgacgcg
    18001 tcggtgttca tttcatggcg ctgattcacg cgctcaatgc gcggccataa acgacgctga
    18061 aaagtggctg tgaggaaata aaacccgtgc accgggcttt tattgcgccg gatgtagtca
    18121 tagcgtgaag taaacagcga gcgcaaaaag taaggcaggc ggttaatcgt ggataaaaca
    18181 ccttgcacct gacgcatctc gtcacgtgta aggggtcttt cgcgcccgac agcctcgcgt
    18241 ggcgcgttcc atgcataagc accggtaaac gccttaccgg tgcctgcagc aaatgctgac
    18301 ggagggacaa aacgcccgga ggctttaacg gtcatatgag ccaaaagcct ctgaacaacg
    18361 cttgctgagt tgctcaacct gcgcgtttaa atcagcaaaa gactttgcgc ttccggtcag
    18421 aatatcgtga tgcatcaggc cggaaacaag ctggcttaa
//
LOCUS       DACAPB010000083        18414 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.083, whole genome shotgun sequence.
ACCESSION   DACAPB010000083 DACAPB010000000
VERSION     DACAPB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.083"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            180..1715
                     /gene="gadC"
                     /locus_tag="IT904_002555"
     CDS             180..1715
                     /gene="gadC"
                     /locus_tag="IT904_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAP3192322.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1846..3165
                     /locus_tag="IT904_002556"
     CDS             1846..3165
                     /locus_tag="IT904_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAP3192323.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3542..4924
                     /gene="dosC"
                     /locus_tag="IT904_002557"
     CDS             3542..4924
                     /gene="dosC"
                     /locus_tag="IT904_002557"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HAP3192324.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4949..7348
                     /gene="dosP"
                     /locus_tag="IT904_002558"
                     /gene_synonym="pdeO"
     CDS             4949..7348
                     /gene="dosP"
                     /locus_tag="IT904_002558"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAP3192325.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFAQRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPYQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7606..8187
                     /gene="ddpX"
                     /locus_tag="IT904_002559"
     CDS             7606..8187
                     /gene="ddpX"
                     /locus_tag="IT904_002559"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAP3192326.1"
                     /translation="MSDTTELVDLAVIFPDLEIKLKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8201..9751
                     /locus_tag="IT904_002560"
     CDS             8201..9751
                     /locus_tag="IT904_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP3192327.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTNKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYMYLFQKNYQLAMNKGVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9753..10775
                     /locus_tag="IT904_002561"
     CDS             9753..10775
                     /locus_tag="IT904_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3192328.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDYWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRIARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10772..11668
                     /locus_tag="IT904_002562"
     CDS             10772..11668
                     /locus_tag="IT904_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAP3192329.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTTRLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11665..12651
                     /locus_tag="IT904_002563"
     CDS             11665..12651
                     /locus_tag="IT904_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3192330.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12644..13570
                     /locus_tag="IT904_002564"
     CDS             12644..13570
                     /locus_tag="IT904_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP3192331.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(13626..14057)
                     /gene="osmC"
                     /locus_tag="IT904_002565"
     CDS             complement(13626..14057)
                     /gene="osmC"
                     /locus_tag="IT904_002565"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAP3192332.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14402..14617
                     /gene="bdm"
                     /locus_tag="IT904_002566"
     CDS             14402..14617
                     /gene="bdm"
                     /locus_tag="IT904_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAP3192333.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14719..14856
                     /gene="sra"
                     /locus_tag="IT904_002567"
     CDS             14719..14856
                     /gene="sra"
                     /locus_tag="IT904_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAP3192334.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15013..16710
                     /locus_tag="IT904_002568"
     CDS             15013..16710
                     /locus_tag="IT904_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP3192335.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16844..17854
                     /gene="adhP"
                     /locus_tag="IT904_002569"
     CDS             16844..17854
                     /gene="adhP"
                     /locus_tag="IT904_002569"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAP3192336.1"
                     /translation="MKAAVVTKDHHVDVTYKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSHTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18000..18284
                     /locus_tag="IT904_002570"
     CDS             18000..18284
                     /locus_tag="IT904_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP3192337.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
BASE COUNT         4619 a         4524 c         4705 g         4566 t
ORIGIN      
        1 cagaacagct ttaaacatac ctgataacgt ttaacggtaa cggtgtcccg aaacgaaccc
       61 gtttcgggac aatttccaaa gtctgttcac tggcattagc aacggaaaat attgttctga
      121 atacgcttca gaacaaaaca ggtgcggttc cgacaggaat accgttttag ggggataata
      181 tggctacatc agtacagaca ggtaaagcta agcagctcac attacttgga ttctttgcca
      241 taacggcatc gatggtaatg gctgtttatg aataccctac cttcgcaaca tcgggctttt
      301 cattagtctt cttcctgcta ttaggcggga ttttatggtt tattcccgtg ggactttgtg
      361 ctgcggaaat ggccaccgtc gacggctggg aagaaggtgg tgtcttcgcc tgggtatcaa
      421 atactctggg gccgagatgg ggatttgcag cgatctcatt tggctatctg caaatcgcca
      481 ttggttttat tccgatgctc tatttcgtgt taggggcact ctcctacatc ctgaaatggc
      541 cagcgctgaa tgaagacccc attaccaaaa ctattgcagc actcatcatt ctttgggcgc
      601 tggcattaac gcagtttggt ggcacgaaat acacggcgcg aattgctaaa gttggcttct
      661 tcgccggtat cctgttacct gcatttattt tgatcgcatt agcggctatt tatctgcact
      721 ccggtgcccc cgttgctatc gaaatggatt cgaagacctt cttccctgac ttctctaaag
      781 tgggcaccct ggtagtattt gttgccttca ttttgagtta tatgggcgta gaagcatccg
      841 caacccacgt caatgaaatg agcaacccag ggcgcgacta tccgttggct atgttactgc
      901 tgatggtggc ggcaatctgc ttaagctctg ttggtggttt gtctattgcg atggtcattc
      961 cgggtaatga aatcaacctc tccgcagggg taatgcaaac ctttaccgtt ctgatgtccc
     1021 atgtggcacc agaaattgag tggacggttc gcgtgatctc cgcactgctg ttgctgggtg
     1081 ttctggcgga aatcgcctcc tggattgttg gtccttctcg cgggatgtat gtcacagcgc
     1141 agaaaaacct gctgccagcg gcattcgcta aaatgaacaa aaatggcgta ccggtaacgc
     1201 tggtcatttc gcagctggtg attacgtcta tcgcgttgat catcctcacc aataccggtg
     1261 gcggtaacaa catgtccttc ctgatcgcac tggcgctgac ggtggtgatt tatctgtgtg
     1321 cttatttcat gctgtttatt ggctacattg tgttggttct taaacatcct gacttaaaac
     1381 gcacatttaa tatccctggt ggtaaaggag tgaaactggt cgtggcaatt gtcggtctgc
     1441 tgacttcaat tatggcgttt attgtttcct tcctgccgcc ggataacatc cagggtgatt
     1501 ctaccgatat gtatgttgaa ttactggttg ttagtttcct ggtggtactt gccctgccct
     1561 ttattctcta tgctgttcat gatcgtaaag gcaaagcaaa taccggcgtc actctggagc
     1621 caatcaacag tcagaacgca ccaaaaggtc acttcttcct gcacccgcgt gcacgttcac
     1681 cacactatat tgtgatgaat gacaagaaac actaaccatg tctgaatggt tataagacaa
     1741 gggagcgata attcatcgct ccctttttcg tgcttgctgt cgtattgact taacggggat
     1801 aagtacgaga atgagcgcac atctgtttac cggaaaccag cacatatgga tatctgctcc
     1861 cgaaacgaga aattagcgat tagaagaccg gcgatactgg ttgcactggc acttttactg
     1921 tgtagttgta aaagcacgcc tccagagtcc atggtgacac caccagcggg ttcaaagcca
     1981 ccagccacga cgcaacaatc gtcacaaccg atgcgtggca tctggctggc cacggtgtct
     2041 cgcctcgact ggccaccggt ttcctcggtt aacattagta atcccaccag ccgggcccgt
     2101 gtacaacaac aggcgatgat cgacaaactg gatcatctgc aacgtctcgg tataaacacg
     2161 gtctttttcc aggtcaagcc ggacggtacc gccctgtggc catcgaaaat tttgccgtgg
     2221 tccgatctta tgaccggtaa gattggtgaa aatccgggtt acgatccgct gcaatttatg
     2281 ctcgacgaag cccacaagcg tgggatgaaa gtacacgcct ggtttaaccc ctatcgcgta
     2341 tcggttaata cgaagcccgg tactatcagg gaactgaata gcactctgtc tcaacaaccg
     2401 gcgagcgtct atgtgcaaca ccgcgactgg atcagaacgt ctggcgatcg ctttgtcctc
     2461 gacccgggca tccctgaggt tcaggactgg atcacatcaa tagtcgcaga agtggtttcc
     2521 cgctatcccg tagatggcgt gcagtttgac gactatttct atacggagtc accgggttca
     2581 cggctaaatg ataacgaaac gtaccgtaaa tacggaggcg catttgcgtc aaaagcagac
     2641 tggcggcgca acaatactca gcagttaatt gcaaaggtat cgcacaccat taaaagcatt
     2701 aagccgggag tcgaatttgg tgttagcccg gcaggcgtgt ggcgtaaccg atcacacgat
     2761 ccgctcggtt ccgatacccg aggcgcggca gcctatgacg aatcctacgc tgacacccgt
     2821 cgatgggtgg aacaaggatt gctggattac attgctcccc aaatttactg gccgttctca
     2881 cggagtgccg cgcgttatga cgtgttggca aaatggtggg cggatgtcgt taaaccgacc
     2941 aggacccgcc tgtatatcgg tatcgccttc tataaagtgg gtgaaccttc aaagatagag
     3001 ccagactgga tgattaacgg cggcgtaccg gagctgaaaa agcagctcga tcttaacgat
     3061 gcggtaccag aaattagcgg caccatcttg ttccgtgagg actatctgaa taaaccgcag
     3121 actcaacaag cggtcagcta tctgcaaagt cgttggggca gttaaactcc cattcttact
     3181 ttgtaacaag ccggtgcttt acccaccggc ttgttaccct cttgaactat cccactagta
     3241 atcatgctta ctaaagtcaa attaaacacg caattaacta cacttagtta aacaaccata
     3301 aatcccctaa taaaaatgta tgaatatgaa aaaacatacg atattcgctg tcaccgaaaa
     3361 ataattacga tgggctatac tcttctgaag aacagtagcg tagattgaaa ctgtcctaaa
     3421 ttgcttattt cttttcagta tatcttcata tttcaggaga atatggtgca gttagtcgat
     3481 ctcgcacgtt gcgtttcgtt ttgattccgt ctgatttccc agccttataa gggtgtgaat
     3541 tatggagatg tattttaaaa gaatgaaaga tgagtggacc ggacttgtcg aacaggcaga
     3601 tccgcccatt cgtgctaaag ccgcggaaat tgccgttgcg catgctcatt atctgagtat
     3661 tgagttttat cgaattgtcc gcatcgaccc gcatgccgaa gaattcttga gtaatgaaca
     3721 agttgagcgg cagttgaaaa gtgcgatgga gcgctggatt attaacgtgc tttctgccca
     3781 ggttgacgat gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag tgcatgcccg
     3841 cataggaatt ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa aaatcctcta
     3901 tccggtaatc ttctcttcgg attattctgc cgcagaaaaa cttcaggtct accatttctc
     3961 gattaacagt attgatatcg ctatggaagt gatgacccgc gcgtttacct ttagtgacag
     4021 tagtgcctca aaggaagatg aaaactatcg tatcttctcg ttactggaaa acgccgaaga
     4081 agaaaaagaa cggcaaatag cctcaatact ttcatgggaa atagatatta tctataaagt
     4141 cctgctggat tctgatttag gcagtagttt gcctttaagc caggctgatt ttggcctgtg
     4201 gtttaaccat aaaggtcgac attattttag cggcattgcc gaagtgggcc atatctcccg
     4261 tctgattcag gatttcgacg gtattttcaa tcaaaccatg cgtaacacca ggaatttgaa
     4321 taacagaagt ctgcgggtga aatttttatt acagataaga aataccgtat cgcaaattat
     4381 taccttgctg cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta tggatgtact
     4441 gacgaaatta cttaaccgcc gtttcctgcc gactatcttc aaacgcgaaa ttgcccatgc
     4501 caaccggacc ggtacaccgc tgtcagtgct gattattgac gttgataaat tcaaagagat
     4561 caacgatacg tggggccata acactggcga tgaaattctg cgtaaagtct ctcaggcctt
     4621 ttatgacaac gtccgcagta gtgattatgt tttccgctac gggggcgatg aatttatcat
     4681 tgtattgact gaagcttctg aaaacgaaac gttacgtacc gcagaacgta ttcgcagtcg
     4741 ggtggagaaa accaaactga aagccgcaaa cggcgaggat attgccctct cactttccat
     4801 cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa tagccgatga
     4861 agctctgtat atcgccaaaa gacgaggtag aaaccgtgtt gaactctgga aagccagtct
     4921 ttagatgcgc caggatgcag aggtaatcat gaagctaacc gatgcggaaa atgccgccga
     4981 tggcattttt ttccccgccc ttgaacaaaa tatgatgggc gcggtgttaa ttaacgaaaa
     5041 tgatgaagtg atgtttttca accccgccgc agagaagctc tggggataca aacgtgaaga
     5101 agtcattggc aataacattg atatgctgat tccgcgggat ttgcgtcctg cgcatcctga
     5161 atacattcgt cataaccgtg aaggcggtaa agcgcgtgtt gaggggatga gtcgggagct
     5221 gcagctggag aaaaaagacg gcagtaaaat ctggacccgt tttgcgctat cgaaagtgag
     5281 cgccgagggg aaagtttatt acctggcgct ggtacgggat gccagcgtag aaatggcgca
     5341 aaaagaacag acccgacaat tgattattgc cgttgaccat ctcgaccgac cggtgattgt
     5401 cctcgatccg gaacgccata ttgtgcagtg caatcgcgca tttaccgaaa tgtttggtta
     5461 ctgcattagc gaagccagcg gtatgcagcc cgatacactc ctgaacattc ctgaattccc
     5521 tgccgataac cgcattcgtt tacaacagtt gctatggaaa accgcccgcg atcaggacga
     5581 atttctgctg ttgacgcgca ccggtgaaaa aatctggatt aaagcctcta tcagcccggt
     5641 ttatgacgtg ctcgcgcatc tgcagaacct ggtaatgact ttctcggata tcaccgaaga
     5701 acggcagatt cgccagcttg aaggcaatat tctcgccgcc atgtgcagca gcccgccatt
     5761 tcatgaaatg ggggaaatca tttgtcgtaa catcgaatct gtactcaacg aatcgcatgt
     5821 ttcgctgttc gcacagcgca acgggatgcc gatacactgg gcgtcatctt cccacggtgc
     5881 agaaattcaa aatgcgcaaa gctggtcagc gaccattcgt cagcgtgatg gcgcgcctgc
     5941 ggggatcctg caaattaaaa cctcgtcagg agcagaaacc agcgccttta tcgaacgcgt
     6001 ggcagatatc agccagcata tggccgcgct ggcgctggaa caggaaaaaa gccgtcagca
     6061 tattgaacaa ctcatccaat ttgatccgat gaccggtctg ccaaatcgca ataacctgca
     6121 caattacctc gatgacctgg tcgacaaagc cgtctctccc gtggtgtatc tcatcggtgt
     6181 tgaccatatt caggatgtga ttgatagcct tggctatgcg tgggccgatc aggcattgct
     6241 ggaagtggtc aatcgctttc gtgaaaaact caaaccggat cagtatctct gtcgtatcga
     6301 aggtacgcag tttgtcctcg tgagcctcga aaacgacgtc agtaacatta cccaaatcgc
     6361 cgatgagcta cggaatgtgg tcagcaagcc gataatgatt gacgataaac ccttcccgct
     6421 taccttgagt attggcatca gctacgacgt gggtaaaaac cgcgattact tgctctccac
     6481 tgctcacaat gcaatggatt atattcgcaa gaatggcggt aacggctggc agttcttcag
     6541 cccggcgatg aacgaaatgg taaaagagcg tttggtttta ggcgcagcgc tgaaagaagc
     6601 gattagcaat aaccaactga aactggttta ccagccgcaa atcttcgcag aaacgggtga
     6661 actgtacggc atcgaagccc ttgctcgctg gcacgatccc ctgcatggtc atgtgccccc
     6721 ttcacggttt attcctctcg cagaagagat tggtgaaatc gaaaatattg ggcgctgggt
     6781 catcgcggaa gcttgccgtc agttagcaga atggcgtagc cagaatattc atatcccggc
     6841 gttatccgtg aacttgtcgg cgctgcactt tcgcagtaat caactgcctt atcaggtgtc
     6901 tgatgcaatg cacgcctggg gtattgacgg ccaccagctg acggtagaaa tcacggaaag
     6961 catgatgatg gaacacgata ccgaaatctt taagcgcatt cagatcctgc gtgatatggg
     7021 cgtgggctta tcggtagatg attttggtac gggcttttcc ggattatccc gcttagtcag
     7081 tcttccggta acggaaatca aaattgacaa aagttttgtc gatcgttgtc tgaccgaaaa
     7141 acgcatcctt gccttacttg aagccattac cagcattggg caaagcctca atttaaccgt
     7201 cgtggcggaa ggcgtcgaaa ccaaagagca atttgagatg ctacgcaaga tccactgtcg
     7261 cgttattcag ggatatttct tttcccgccc cctacccgcc gaagaaattc caggctggat
     7321 gagcagcgtg ttaccgctga aaatctgaca aattcctctc gcccgcactc gcgggttttc
     7381 atttaacgtg acaccgtcac ttaaatactg tgatttcagc tacgattccg ggagattcct
     7441 tccttaactt cccctcttgc accaaaactg cactacgctg cacaaatgtt gatcaaaagt
     7501 taaaaaatat aactttaagc cagcattctg gcgcttatcc cggcatggca tgagatctgc
     7561 ataagcggaa agcgcagcaa tttttgtctt atacaggaac cgtttatgtc ggataccacc
     7621 gaactggttg atttagccgt aatcttccct gatctggaga tcaaattgaa atacgcctgc
     7681 gctgataaca tcacaggtaa agctatttat cagcaagcgc gttgtctgtt acacaaggat
     7741 gcgattaccg cgctggcgaa aagtatcagc atcgcccagc tgtcagggtt acaactggtg
     7801 atttacgatg cgtatcgccc acaacaagca caggcgatgt tgtggcaagc ctgcccagac
     7861 ccgcaatatg ttgttgatgt gacggtcggt tctaatcaca gccgtggcac ggcgatcgac
     7921 ctgacgcttc gtgatgagca cgggaacatc ctcgatatgg gcgcagggtt cgatgaaatg
     7981 cacgagcgct cccatgccta tcacccttcc gtcccgcccg ccgctcagcg caatcggctg
     8041 ttgctgaatg cgataatgac tggtggcggt tttgtcggta tctccagcga atggtggcac
     8101 ttcgaattac cccaggcagc gagttaccct ctgctcgctg atcaattctc ttgttttata
     8161 tcccctggca cacagcacgt cagttaatca ggaaactgtc atgaagagat cgatatcgtt
     8221 tcgtcccaca ttgctcgcgc tcgtccttgc cacaaatttc ccggttgcgc acgccgccgt
     8281 accaaaagat atgctggtga ttggtaaggc cgccgatcca caaaccctcg acccggcggt
     8341 aacaatagat aataacgact ggacagtgac ctacccgtct tatcagcggc tggttcagta
     8401 caaaacggac ggtgataaag gctcaaccga cgttgaaggc gatctggcaa gtagctggaa
     8461 agcgtctgac gatcaaaaag agtggacgtt caccctgaaa gataatgcta aatttgccga
     8521 tggcacacct gtcactgccg aagcagtaaa actttctttt gagcggctac taaaaatcgg
     8581 ccaggggcca gcagaagcat ttcccaaaga tttaaagatt gatgctcccg acgaacatac
     8641 ggtgaagttt acccttagcc agccattcgc accgttcctc tacacgctgg cgaatgacgg
     8701 tgcatccatt atcaatccgg cggtgttaaa ggaacatgca gcggatgatg cccgcggctt
     8761 cctcgcgcaa aataccgccg gttccggacc atttatgctg aaaagctggc aaaaaggtca
     8821 acaattagtt ctggtgccaa atccgcatta ccctggcaat aaaccgaact ttaagcgagt
     8881 atcggtaaaa attattggtg aaagtgcctc ccgtcgcctg cagctctccc gtggtgatat
     8941 tgacattgcc gatgcgctgc cggtggatca actcaacgcc ctgaagcagg aaaacaaagt
     9001 caatgtggca gagtatccgt cactgcgcgt cacctacctg tatctgaata acagcaaagc
     9061 gccacttaat caggcggatc tgcgtcgggc catttcctgg tctaccgatt accagggaat
     9121 ggttaacggc attctgagtg gtaacggaaa acagatgcgc ggcccgattc cggaaggcat
     9181 gtggggctac gatgcgacgg caatgcaata caaccatgac gaaacgaaag ccaaagctga
     9241 atgggataaa gtgacgaaca aacccaccag cctgacgttt ctctattctg ataatgatcc
     9301 gaactgggag cctattgctc tggcgacaca atccagtctc aacaagctgg gcatcaatgt
     9361 gaagctggaa aagctggcga acgccaccat gcgcgacaga gtgggtaaag gtgattacga
     9421 cattgcgatt ggcaactgga gtccggattt tgccgacccg tatatgttta tgaattactg
     9481 gtttgagtca gacaaaaaag gtctgccggg taaccgctcg ttctatgaaa acagtgaggt
     9541 cgataagtta ctgcgcaatg cgcttgcgac caccgaccag acgcagcgta cccgggacta
     9601 ccagcaggca cagaaaatcg tcattgatga cgctgcttat atgtacctgt tccagaaaaa
     9661 ctaccaactg gcgatgaaca aaggggtgaa aggctttgtg ttcaatccca tgctggaaca
     9721 ggtcttcaat atcaatacca tgagtaaata acgtgacctt ctggagtatt ttacgccaac
     9781 gctgctgggg gctggtgctc gtggtggcgg gcgtctgcgt gattacgttt attatctcgc
     9841 atctgatccc tggtgatccg gcgcggttac tggcgggtga ccgcgccagc gatgctatcg
     9901 tggaaaatat tcgccagcaa ctgggactgg accagccact gtatgtacag ttttaccgct
     9961 acgtcagcga cctgtttcat ggtgacctgg gaacatccat tcgtaccggg cgtccggtgc
    10021 tggaagagtt gcgtatattt ttcccggcga cgctggagct ggcattttgc gcgctgctac
    10081 tggcactcct gattggcatc ccgctgggca tactctctgc agtctggcga aatcgctggc
    10141 tggatcatct ggtgcgaata atggccatta ccggaatctc cacacctgcg ttctggcttg
    10201 ggctgggcgt cattgtgctg ttttatggtc atctgcaaat tcttcccggc ggcggaaggc
    10261 ttgattactg gctggatcca ccaacgcacg ttaccggctt ttatctgctc gatgcgctgc
    10321 ttgaaggtaa cggtgaagtc ttcttcaatg cgctgcaaca tctcatctta ccggcattaa
    10381 cgctggcgtt cgttcacctg ggaattgtcg ctcgccagat ccgctcagcg atgctggaac
    10441 aattgagtga agactacatt cgtatcgccc gggccagcgg cttgcccggc tggtatatcg
    10501 ttctatgtta tgcgctaccc aatgcgttga tcccatcgat taccgtattg gggctggcgc
    10561 tgggcgattt gttgtatggc gcagtgctca ccgaaaccgt ttttgcctgg cccggaatgg
    10621 gtgcatgggt agtaacatca atacaggcgc tcgacttccc ggcagtgatg ggctttgccg
    10681 tcgtggtttc atttgcttat gtgctggtca acctggtggt ggatttgctc tatttgtgga
    10741 ttgatccgcg tatcggacgt ggaggtggtg aatgatgcta agcgaggaaa cgtccgccgt
    10801 acgcccacaa aaacaaacgc gatttaacgg tgcaaaactg gtatggatgc tgaaaggcag
    10861 tccgctcacc gtgaccggcg cagtcatcat tgtattaatg ctactgatga tgattttttc
    10921 accgtggctg gcgacgcatg atcccaacgc cattgattta accacccgcc ttttgccgcc
    10981 ttctgcggcg cactggtttg gcaccgatga agtgggacgc gatctgttta gccgcgtact
    11041 ggtcggcagt cagcaatcaa ttctcgccgg attagtcgta gttgccattg cgggtatgat
    11101 tggttcgcta ctcggatgtc tatccggtgt gcttggcgga cgcgcagacg ccatcatcat
    11161 gcgcatcatg gacattatgc tgtcgattcc ttcgttggta ctgacaatgg cactggctgc
    11221 cgctctcggg ccgagtttgt ttaacgccat gctggcgatt gctatagtgc gaattccgtt
    11281 ttatgtgcgc ctggcgcggg ggcaagcatt agttgtacgc cagtatacct atgttcaggc
    11341 ggcgaaaacc tttggcgcgt cgcgttggca tctgatcagc tggcatattt tacgtaattc
    11401 cctgccgccg ctgatcgtgc aggcatcgct ggatatcggt agcgcgattt taatggccgc
    11461 cacgttggga tttattggcc tgggtgctca acaaccgagt gctgaatggg gcgcgatggt
    11521 agcaaatggt cgcaactatg tactcgatca gtggtggtat tgcgcatttc cgggggcagc
    11581 gattttgctt accgccgtcg ggtttaatct ctttggtgat ggtattcgcg atctgcttga
    11641 cccgaaagca ggaggaaagc agtcatgacc caacccgttc tggacattca acaactgcat
    11701 ttgagtttcc ccggttttaa cggtgatgtt cacgcgctca acaatgtgtc cttgcagatt
    11761 aaccgcggtg aaattgtcgg tctggtggga gaatccggct caggtaaatc agtcaccgca
    11821 atgctgatta tgcgtctgct accgacgggc agttattgcg tacatcgggg gcagatttcc
    11881 ctgctgggag aagatgttct taacgcgcgg gaaaaacagc ttcgtcagtg gcgcggcgca
    11941 cgagtggcga tgatctttca ggaaccgatg accgccctca atccgacacg tcgaataggt
    12001 cttcagatga tggacgtgat ccgccatcat caaccaataa gtcgtcggga agccagagct
    12061 aaagcgattg ccctgctgga agagatgcaa atcccggatg ccgtggaagt tatgtcgcgc
    12121 tatccgtttg agctttcagg tggtatgcgc cagcgggtaa tgattgcgct ggcattctcc
    12181 tgcgagccgc aattgattat tgccgacgaa ccgactacgg cgctggacgt cacggtgcag
    12241 ttgcaggtac tgcgtttgct taaacataaa gcccgcgcca gtggaactgc ggttctgttc
    12301 atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata gcgtttacgt gatgtatgcc
    12361 ggaagcgtaa ttgaaagcgg cgtgacggca gacgttatcc atcatccccg gcatccgtat
    12421 accattggtt tgctccaatg cgcaccagaa catggagtac cacgccagct attacccgcc
    12481 attccaggga cggtaccaaa cctcacccat ttgcctgacg gctgcgcttt tcgcgatcgt
    12541 tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc tgacagcgtg tggtgacaac
    12601 aaccagcgct gcgcctgttg gtatccgcag caggaggtca taagtgtctg acacgttatt
    12661 aacgttacgc gacgtccata tcaatttccc ggcccgtaaa aactggcttg gtaaaactac
    12721 ggaacatgtt catgcgatta atggtattga tttacagatc cgccgtggtg aaaccttagg
    12781 gatcgtcggc gagtcaggct gcggcaaaag caccctcgca cagcttttaa tgggtatgct
    12841 gcaaccgagc cacgggcagt acatccgttc tggctcacaa cgcattatgc agatggtgtt
    12901 tcaggacccg ctctcttcgc tcaatccgcg cttaccggtg tggcgcatca tcacagaacc
    12961 gctctggata gctaagcgta gtagtgaaca acagcggcga gcgttggcag aggagctggc
    13021 tgtgcaggtg ggtattcgtc cggagtatct cgaccgcctg cctcatgcgt tctccggcgg
    13081 gcagcggcaa cgtatcgcca ttgccagagc actctcttcg cagcctgacg tgattgtgct
    13141 tgatgagcca acctctgcgc tggatatctc cgtgcaggcg cagatcctca atttactggt
    13201 aacgctacag gaaaatcacg ggctgaccta tgtgctgatt tcacacaatg tctcggtgat
    13261 acgtcatatg agcgatcggg tggcggtgat gtatctcggg cagattgtag aactgggcga
    13321 cgcgcagcag gtgctgacgg cacctgcaca tccatacacc cgattattgc tggattccct
    13381 ccccgccatt gataaaccgc tggaggaaga atgggcatta cgtaaaacgg atctgccggg
    13441 aaaccgcacg ttgccgcaag gctgtttttt ctacgaacgt tgcccgctgg caacccacgg
    13501 atgtgaagtc cggcaatcat tagcaataag ggaggacgga cgtgagctcc ggtgctggcg
    13561 ggcgctgtag agatgctaac aacgcatcag gcattcacgc gcctgacgcg tcatccggca
    13621 atgctttacg atttcaactg gtaatccagc gtaatttccg ctttcagtac ctgagagacc
    13681 gggcatcctg cttttgcttt ctggatgatg ccgtcaaagg tagaggcatc aatacccggc
    13741 accgcaactt cactcttcag tgcgattttc gtaatcgcaa aaccggcatc cactttatcc
    13801 agcgacacat cggcggtggt atcaatcgat gttggcgtga atcccgcttc ccccagcatt
    13861 aatgaaagcg ccattgagaa acatgcggca tgcgctgcgc caatcagttc ttcagggttg
    13921 gttccttttt cgccttcaaa acgcgtgtta aatccatacg gctgttggtt cagcacgcca
    13981 ctctcggtgg atactgttcc cttcccgcgt ttgatatcgc cttcccagtg tgcctgacct
    14041 ttcttatgga ttgtcattgt tgctctcctg tgggcttaaa aaggaaatta tagactacgt
    14101 gagaaacagc acgaggataa gcaggatatt ccgaataaaa tccggcgaaa atattacttc
    14161 tgcacttaaa ttaaattccc acaatagatt accgggaata tataacccaa aagatattgt
    14221 atcaatatta atcggatcat accaatcaca atccctatga atatcatggt gttaaaatat
    14281 aaaaatcatc aaccaggact aatcttaaca acgaagcggc aaatatttgc catgctgaat
    14341 gtgctttata aatctgcgat ccgtagcaga caccataaat acacagacac ggagaatcac
    14401 tatgtttact tattatcagg cagaaaattc aacagcagaa cccgctctgg ttaatgccat
    14461 tgagcaaggc cttcgggcac agcacggtgt tgtcactgaa gatgacattc tcatggagct
    14521 gaccaaatgg gttgaagctt ctgataacga catcctcagt gatatctacc agcaaacgat
    14581 caattatgtg gtcagtggcc agcaccctac gctttaaggt gctatgcttg atcggcaacc
    14641 taatttaggg gtttagcacg tgtttcttcg ctacggcgat gttgtcctta aaactagcta
    14701 caggattgag gagttaaaat gaaatcgaac cgtcaggcac gtcatattct tggactggac
    14761 cataaaattt ctaaccagcg caaaatagtt accgaaggtg acaaatccag cgtagtaaat
    14821 aacccaaccg gcagaaaacg ccccgctgaa aagtaattca taaccatcag tcctcaatga
    14881 cgattaaaca ccattgcctg cgcaatggtg tttttgtttt tatctgcttt atacttgagg
    14941 ccgacgccct ggcggtaaag caaagacgat aaaagccccc cagggatgga tattcaaaaa
    15001 agagtgagtg acatggaacc aaaaacaaaa aaacagcgtt cgctttatat cccttacgct
    15061 ggccctgtac tgctggaatt tccgttgttg aataaaggca gtgccttcag catggaagaa
    15121 cgccgtaact tcaacctgct ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa
    15181 gcggaacgag catggatcca gtaccaggga ttcaaaaccg aaatcgacaa acacatctac
    15241 ctgcgtaaca tccaggacac taacgaaacc ctcttctacc gtctggtaaa caatcatctt
    15301 gatgagatga tgcctgttat ttatacccca accgtcggcg cagcctgtga gcgtttttct
    15361 gagatctacc gccgttcacg cggcgtgttt atctcttacc agaaccggca caatatggac
    15421 gatattctgc aaaacgtgcc gaaccataat attaaagtga ttgtggtgac tgacggtgaa
    15481 cgtattctgg ggcttggtga ccagggcatc ggcgggatgg gcattccgat cggtaaactg
    15541 tcgctctata ccgcctgtgg cggcatcagc ccggcgtata cccttccggt ggtgctggat
    15601 gtcggaacga acaaccaaca gctgcttaac gatccgctgt atatgggctg gcgtaatccg
    15661 cgtatcactg acgacgaata ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa
    15721 cgctggccag acgtgctgtt gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta
    15781 cttaaccgct atcgcaatga aatttgttct tttaacgatg acattcaggg cactgcggcg
    15841 gtaacagtcg gcacactgat cgcagcaagc cgagcggcag gtggtcagtt aagcgagaaa
    15901 aaaatcgtct tccttggtgc aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc
    15961 cagacccagc gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt tatggtcgat
    16021 cgctttggct tgctgactga caagatgccg aacctgctgc ctttccagac caaactggtg
    16081 cagaagcgcg aaaacctcag tgactgggat accgacagcg atgtgctgtc actgctggat
    16141 gtggtgcgca atgtaaaacc agatattctg attggcgtct caggacagac cgggctgttt
    16201 acggaagaga tcatccgtga gatgcataaa cactgtccgc gtccgatcgt gatgccgctg
    16261 tctaacccga cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt
    16321 aacgcgctgg tcgccacggg cagcccgttt aatccagtgg tatggaaaga taaaatctac
    16381 cctatcgccc agtgtaacaa cgcctttatt ttcccgggca tcggcctggg tgttattgct
    16441 tccggcgcgt cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac gctggcgcag
    16501 tattcaccat tggtgctgaa cggcgaaggt atggtactgc cggaactgaa agatattcag
    16561 aaagtctccc gcgcaattgc gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg
    16621 gtgaaaacct ctgccgaagc cctgcaacag gccattgacg ataatttctg gcaagccgaa
    16681 taccgcgact accgccgtac ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc
    16741 tatttccctc tccctttttc agatctcatc catactgggt agtggcgaat aaatctcatt
    16801 tgcctcacct gctatgcaga acatcatccg aaaaggagga actatgaagg ctgcagttgt
    16861 tacgaaggat catcatgttg acgttacgta taaaacactg cgctcactga aacatggcga
    16921 agccctgctg aaaatggagt gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg
    16981 cgattttggt gacaaaaccg gcgtaattct gggccatgaa ggcatcggtg tggtggcaga
    17041 agtgggtcca ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta
    17101 cgaaggatgc ggtcattgcg aatactgtaa cagtggtaac gaaacgctct gccgttcagt
    17161 taaaaatgcc ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga
    17221 ttacgcggta aaagtgccag atggtctgga ctcggcggcg gccagcagca ttacctgtgc
    17281 gggagtcacc acctacaaag ccgttaagct gtcaaaaatt cgtccagggc agtggattgc
    17341 tatctacggt cttggcggtc tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa
    17401 cgccaaagtg atcgccattg atgtcaatga tgagcagtta aaactggcaa ccgaaatggg
    17461 cgcagattta gcgattaact cacacaccga agacgccgcc aaaattgtgc aggagaaaac
    17521 tggtggcgct cacgctgcgg tggtaacagc ggtagctaaa gctgcgttta actcggcagt
    17581 tgatgctgtc cgtgcaggcg gtcgtgttgt ggctgtcggt ctaccgccgg agtctatgag
    17641 cctggatatc ccacgtcttg tgctggatgg tattgaagtg gtcggttcgc tggtcggcac
    17701 gcgccaggat ttaactgaag ccttccagtt tgccgccgaa ggtaaagtgg tgccgaaagt
    17761 cgccctgcgt ccgttagcgg acatcaacac catctttact gagatggaag aaggcaaaat
    17821 ccgtggccgc atggtgattg atttccgtca ctaagaggcc tttgctgcga ctgccatgtt
    17881 cgggtcgcag catcgcacac tctccaacat gaaatggcta aatggattat tgttgcgtag
    17941 gaaggaacaa taatgatagt gttcccagtg aaggaacgct actgagagta aagacactaa
    18001 tgaaaatggc caatcatccc cgcccggggg acattattca ggaatcactg gacgaactta
    18061 atgtcagcct gcgcgagttt gccagagcaa tggaaattgc tccctcaacg gcaagccgat
    18121 tgctgacagg aaaagcagct ttgacgccag aaatggcaat taaactttcc gtggtgatcg
    18181 gcagttcgcc gcaaatgtgg ctgaatctgc aaaacgcctg gagtctggca gaggcagaaa
    18241 aaacggtgga tgtgtcgcga ttacgccgtc tggttacgca ataaaaaaat ggcagtttat
    18301 aaagcgccat ttagaactga tgacaaacgc aacattgcct gatgcgctac gcttatcagg
    18361 cctacacgtt cactgcaatc tattggattt gaaggggttg taggccggat aagg
//
LOCUS       DACAPB010000084        18296 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.084, whole genome shotgun sequence.
ACCESSION   DACAPB010000084 DACAPB010000000
VERSION     DACAPB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.084"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..896)
                     /locus_tag="IT904_002571"
     CDS             complement(<1..896)
                     /locus_tag="IT904_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAP3192338.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEDVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVNPGVLNGRYRVTFTNGNHIT"
     gene            complement(897..5291)
                     /locus_tag="IT904_002572"
     CDS             complement(897..5291)
                     /locus_tag="IT904_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAP3192339.1"
                     /translation="MRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRI
                     VVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKP
                     SRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPL
                     GQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEIL
                     WTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLY
                     IFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDA
                     RNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTG
                     EAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDAS
                     GHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGE
                     SVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDK
                     DGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLER
                     DRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAG
                     IKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEI
                     APNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREE
                     VDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLY
                     MKWSMPTVYRVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQ
                     NIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASN
                     IRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQ
                     MIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQH
                     MLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIR
                     DINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAH
                     TVVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAK
                     HKLTGGGEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYR
                     LIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERT
                     TWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVL
                     PPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(5435..6058)
                     /locus_tag="IT904_002573"
     CDS             complement(5435..6058)
                     /locus_tag="IT904_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAP3192340.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(6055..7743)
                     /locus_tag="IT904_002574"
     CDS             complement(6055..7743)
                     /locus_tag="IT904_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAP3192341.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVGSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(7892..10519)
                     /gene="gyrA"
                     /locus_tag="IT904_002575"
     CDS             complement(7892..10519)
                     /gene="gyrA"
                     /locus_tag="IT904_002575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAP3192342.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEASRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            10666..11388
                     /locus_tag="IT904_002576"
     CDS             10666..11388
                     /locus_tag="IT904_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAP3192343.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEHILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(11516..15268)
                     /gene="yfaL"
                     /locus_tag="IT904_002577"
     CDS             complement(11516..15268)
                     /gene="yfaL"
                     /locus_tag="IT904_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAP3192344.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVMLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            15964..18249
                     /locus_tag="IT904_002578"
     CDS             15964..18249
                     /locus_tag="IT904_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 1 subunit
                     alpha"
                     /protein_id="HAP3192345.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4294 a         5077 c         4473 g         4452 t
ORIGIN      
        1 gtaatgtggt taccattagt aaacgtcaca cgatagcgcc cattaagtac gccgggattt
       61 actgccgtcg tactcttttc cgctgtgatt ttcttcagcg gatagcgggc aaacagctcc
      121 acctctacac attgcccact ggcaacctgc gtcggcactg gcaaaacccg attaagcacc
      181 gtcgcataac gggttagtac cgttttgctg gtgccactgc cagtaaccca cagcggtgta
      241 ccatcggtta accagccagc aaaaccaccc tgacggattt cctgtttatc atcggcaaac
      301 cagctccagg ttttcacccg tacccgacta ccgagcatgg atgcgacacc gattttcgcc
      361 tcgtccagca ccacatccag taagacttcc tgagctttgt tttgcgcggg taacgtcgct
      421 aatgaatcaa gcagcgattt aaccgttacc gaggtttctg gcttcagcgt agtcagagac
      481 gctaaccacg caggtgcctg acgctgttgc cagtattgcc cccacacatc tgcgccaata
      541 tgcaaccgct gcggcgcaaa atagagtcca caggaacgta ccagcgcggt atcgcgcgtt
      601 atgctctccc cagcctgaca acaataaaca tcttccgggg agtttccacg acactggtag
      661 ggctgctccg gttgatgggt atcctccagc caggcgtaaa catacagttt ccacaaactc
      721 cctaacggcg tggtgagcgt gtcaggtaat gccgagactt tggtcacgcc tgatgacgac
      781 aactgataca gttgatcgtg ctgcgcgccc cgcagcgcca gttgtaacgg cgtctcctcc
      841 gcaagcgttg gcagagtaac caacgccaat agccagacga ttctgcgcca gttcacttat
      901 ttcacctgca tcccggtcca ttcactccct gccgcaacac tttgctgtgc aggtgcatag
      961 gaacgcacat aacgagcagg aggcagaacg aattgccctt tttgcgagaa gcgcagcaaa
     1021 tggcggaaag tgaccgttcc cgtcagttct ttcaccggca ccatataggc cagttcgccc
     1081 atttcatttc gcgctttttc cagcaattgc ccctgctgtt tcgcggcgtt gggtttattg
     1141 accgaaatgc cccatgttgt gcgctcaacg tcggctcccg gtgggagcgg tacttccacc
     1201 tgaccatagc gcagaactgc atcctgctcg ctggtaagcg tgatttcatc gagatacagc
     1261 gcatcgctgt caatctcatt gctggtcacc ggttgcagag taaagctcat ctcttcttca
     1321 ccagggataa gccgatacaa ctggcgttca acggtcaccg gaatgttact ttgttgagcc
     1381 gtttttgccg gttcacgcca gcggacctgc acattttgcg gggataattc gtcaccaaaa
     1441 gagagaatgt ccggcacacc ctgaccaacc caacgccagt cttcgccccc tccagttaac
     1501 ttatgtttag cccatgcgcc cgcaggcgca ggcaacacaa ccggaggcat tgtcgccata
     1561 tatttcgcca gccagttcat ggccagtgcg cgctcaatag tggattgctc agcggtcaaa
     1621 ccacttaaaa tagcggccgc ctgggtagcg tcgcccccgc tattgagcag cacaaccgtg
     1681 tgcgccagcg gctgattgct gtttgccgct ttatcccacg catattgctg aacctgtggc
     1741 atcaccgtgg acttcaactg cgcttttttc agcaacgtca tggttaagac gtttgccacc
     1801 gcatctgcca gtggagattc cggtgtatcg aggatcaggc tgtcattgat atcgcggatg
     1861 tcttcctcag agaaatcttc atctttagtt ccgcgccggg cgatagcttc atccaaccct
     1921 ttcaacaacg ttttgcacgg cagattcatc tcctgtgccc acgccagcac cagcgcccga
     1981 tgcaataacg gcatgttatc cgcctgctcg gcataactgt cgagcatatg ctgccagtat
     2041 tccggttgtt gcgttacgcc gagcgcctgg ctggcctgcc agtcggcgta ccatgcccat
     2101 gccgtaagga aggcgtcacc attgccatct tcaccccacc aggtaaagcg cgctccgggc
     2161 cccgccagtt gcatcagccg cagacggtta tcctgaatca tctgacgaat gtcgttagcg
     2221 gcggcacttt gatgatcggc aagcgaacgc caggcgagac tgagcgggat cagacggcta
     2281 ccggtgttga tcaccccacc ccacggttca tcgactaacg catcaagatt gttgcggaaa
     2341 atctcctgcg gcgtttcact actttgtagc cggatattac tcgcctgctc gggcaacatc
     2401 agcgcgttct cgccaccgcc gagcatgaca tttttctgct gttcaacggg ccagctgttg
     2461 tccacaaaag acagttttgt gctaatgctg tcctgcactt gcccattttg ttgcagttct
     2521 gcacttaaca agccagattg ctgaatattc tgcgtcagcg aaatataatt cgcccctttg
     2581 tgcagcgtca gcgtctggcg catctcagcg cctgcaaatt tagtcaccag cgccaccggt
     2641 tcgttatcct gctgactgaa gataaacagt cctgccgccg gtttgtcgcc cacgcgatac
     2701 accgttggca tactccactt catgtagaga tttttttccg aacgcagata agcacgcccc
     2761 tgcccgacca gcccgtcgcc gttcatccca cgcgcggtga tacgccagcg ggttaacgaa
     2821 tcaggcatca ggaacgtgaa atacgctttg ccttgtttat cggttgtgag tgacggcatc
     2881 catgccgcgg tatccacctc ttcacgccgt ggacgttcaa gcatttttac tcgccgctcg
     2941 ctgcggttag tcgcgccagg cgcaaccggc tcgctggaga gcgcctggtc gtagctgata
     3001 aacgacagac tggagctggt acgcacattg ttacgcccca gcggatagaa aaatttgccg
     3061 atattcggcg cgatttctgg ttgcagcgcg tagatcattt catcgaccac gcctaccgtt
     3121 agctgcgcag aaacaggttt acctttcagc gacgaggtta attcgacatt gaccagttca
     3181 ccaggctggt aatgggtttt gtccgttttc acccggatat caagctgagg aacggcaact
     3241 ttgatcccgg cgttctgaaa actgtactga ccattacggg tatacagcac cgaaaaagtg
     3301 atgttaggcg caaaggaatt gctcactgga acccgggctt catactgggt atcgtttaaa
     3361 cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg actgctgttc cacgcgatcg
     3421 cgttccagcg tcaataatgc ttcatcaatt ggctccggaa aggtaatcag catcttcgcg
     3481 gtttcgcctg gctggtacag cgttttatcc gccacgatat ctaccgtacc agtatgcgcc
     3541 gtgctgccct taccgctgac ggcatgactt aacccagcga gaattaagcc gtctttatcg
     3601 cgtaatgtca gattgtagtt gccaggttta gcgaaattga cggtaaagga tttgccgcct
     3661 gacggtagct ctccgctatg gctcgtgcgg tcttcgagac gcaaccattc atacgtaaca
     3721 ggaacctgtt ttgaagattc cagcgcggca taacggaaca caaccgactc gccgctatta
     3781 ctgtattgtg cggcagtact taatgagtaa tgcgccagac cgcgttcaat gaggatctct
     3841 ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg agacggttaa caaatagcgg
     3901 ctcggtttat cggcggcggg gagatttaac gccacatgac cgctggcgtc ggacaccgtt
     3961 tcgctgcctt ccagcgacac ggggaaacgt ccggcataac gcaaatcgtt accgaccatt
     4021 gataattgct gagcgcgcaa acttaactgc acgcgggcat tttttaccgg ctcgccatcc
     4081 gggtagagaa gttgcagttt gccgctgacc gcttcgccag ttttgaactc ttttttgtcg
     4141 agagctaaac caatctcgaa atgtggcttg atgtagtttg ccacgcgaaa actgctgcta
     4201 tagacctgat tgcggtaagc aagacgtaac tcataacctc cggctacggc attttctggc
     4261 aggcggaaac ttccctgccc gccattgcgc gcatccagcg tgacatcgac ggtttgcaac
     4321 agactgccgt tagcgtccag caccgaaagc ttcgccgggg cgctgacgat gggggatgaa
     4381 tgcaacggat cgtggaactc gcggcccatc actttaacat cgacacgatc gcctgcgcga
     4441 tatagcgggc gatcggtaaa aatatacaag cgggtgttgt agatttcgct ttcgtagaag
     4501 aagttctcgg agacaaaaac gccgccttca gcatccttac ccagaatgta tgaacgttct
     4561 ggcgatatat gttgtaactg caaggtaccg ctgtcatcgg tcacaccgcg ggtcatcacg
     4621 ccaagaccgt cagtccacaa gatctctgag ccgggcttcg cttcaccctg ttttttaccg
     4681 gcggtccata ccagaagctc gttacctgac actttgctaa gcgccacggt atcggaaaca
     4741 aacaccaccg tcgtcgcccg atacccacca accatcgcct cgacgaggta cagtcccggc
     4801 tcttgttggc cgagaggaat ataaatgtta cccggctgcg gcgagatgaa attgctggat
     4861 gcgccttcca gttttacccc ttgctgcggc tcgaccggtt tagcctgcca tagtggataa
     4921 cggaactgtt ccaccaaggg atattttttc agcggggaaa actggttgtt ctgtacataa
     4981 cgggaaggtt taataatggc attgccgaga tgtaattcgg gtaatgcctg agtcacattc
     5041 tgccgtgact gagaagagaa agtacgctgc atcacgcggc gagatttgcc gtaccagtta
     5101 tcccacagcc aggtcagcgt attgttcagc ccgtcgccca gatattgcgg ttgcaccaca
     5161 atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg ggtcaggaat acgatacagg
     5221 cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc gataatcacg ccccggcgct
     5281 tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc tgtcagcaag caaaaagaat
     5341 gttcccccgg cgggcggcgc atagttactg gaaggaagcg aatcatcagc attagcaagc
     5401 cctgttccca caagagacag ggacaagcac gctgctatcg cgccagaaaa tttaaacgat
     5461 aaatgccaat gaaattggga ttggaatcgt tgggtatcca tcgggtgtcc ttccatgtca
     5521 taagttgttg cagactgact gcgcgcattc cgttgtcagt tttcgtggcg cttccggtgt
     5581 ggtagatgac gtaacgcccc atccagacca ttaagtgctg ggcatcgccc tgatcgaaaa
     5641 aaatcatatc gccaggcagc gcctggttta tgtcctggcc aataaactgg ctgttgtact
     5701 gaatcaaatt aatcgcggtc acgtaggggc cggttttccc gttcccctga ttccagtttt
     5761 gcgccagttg acgctgtcca ggtgttagcg tcatctctgg cggcaaatac tggctagaga
     5821 aaccgttact ttttagccat ttactatcgt gaactttcag cgtctcgttc gccgcaaatc
     5881 gcaccaggcc cgcacaatcc tgctgatacc agcgtggact tggcccctgg cggagctgtt
     5941 cttgtgcaat gcgtacaaac caggcgcgaa acagcccgga ttgttcgaca ttcagcattt
     6001 cgctatgggc aacaacacaa cacagccagc aaatcagcgc cagcagcccg tgcctcatag
     6061 cggctgccag gttatgggta gccactgcca ggcggcaccg ggttccatct gggccagctt
     6121 catgacataa cgcggttgtt gagataaagc gtccagcttc ggcattaata aagtttgtgc
     6181 ggcgttataa aaaaccggtt cgagattctt cggcagactg gtcaacgttt cgttacgcag
     6241 cagtttcgcc acgccttgtg gattgatata gagcggaacg atgccatcag tgggtattac
     6301 atccaccatt gccgggcggt ttttattcag tgtttgcagg gcgttattaa ccagcgtgtc
     6361 atcgagggag aaaagcagcg ttttgttttg catcgccagc gacacgcgga aaaaataatc
     6421 cgacattaat tgatcgggtt gcgccgcctg cgctttcgga tactgaccgt atcgggaact
     6481 cacttcgcga cgccagattt gcgcttcgcc ctgctgagtc tggcttatcg gcaaaacacc
     6541 ttctggcgct ttgctttcgt gcgcaccaat attttgcgta aacagtttcc ctggcaattg
     6601 cgcctgttcg gcagtgccat caaactgacc gacaaacagc ggggtttgca attttgagtc
     6661 ttcataccag cacagccccg cggcaccgtc taacgcccca ttcagcttgt cgttttcctg
     6721 gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa tacggcaccg ccacacagaa
     6781 gctggccccg gcaggcatac tgttccagac cggcgtaaaa tcgaaactgg tatctacgct
     6841 ggcggattca tcatttaacg ccacaaaact gtgccagccg tcgttaccca tttcgaagcg
     6901 tacgccagca aaagaaggca ttaaccgctg gtagccaaaa cccagcaacc tggcgctgcc
     6961 cacgatgcgc tggcgtactg gcgttttttc agcagcacgc tcttccaggc caaagcttgc
     7021 ttgccagcgt tttttgccgc tcaacaaatc acctgcgatc gccgtggctt cggtatcctg
     7081 ctgatcatct ttaaacaaca tatccgtgct ggaaaacacc agcatcttgt cctgataagt
     7141 cgcgaacatc agggcgttat tgccgttata gcgcaactga taaacaggaa tagtttcact
     7201 atttatcttg atgctactga tttccgtttt gcttaactgg ctgtcgctgg tagcggcaaa
     7261 caacaatggc tccagcagtt tgcttaaacc actgcgctgg atcagcacca tataatgtga
     7321 aagatggcct tgtttatcgt gccacagcgc tgcctgcgcg ggctgatcta agagtgacga
     7381 aaagagctta tctttcagcg tgagatcgtg ttcatagaca atacgacgaa tgctgccttc
     7441 aatgcccaga cgatcggcat gattctgata atagaaaacg aaatcttcgc tcagaacatc
     7501 gtggagaaac ggaatggtga ggagatcttt gggaagctgg ctcagagagt cgctgtcgag
     7561 aaagaggtcc ggctcattga gatcgatttg cagattgttg tgcaccacca gcggcgacaa
     7621 cgttttttct ggcccactgc cagcatattg caacgcccag acgccagcgg aaagcagtgc
     7681 tattgcgcca aaacctacaa gaccatagaa ccgccagcct ttcgcctttt tttcaccact
     7741 cattgccaca ttccttgtgt atagccagcc attttttacg ggcacagcca aactttaccg
     7801 tgccctaata cgacaaaagc ccagactttg cagcctggac ttttcaattc aaacaaggga
     7861 gatagctccc ttttggcatg aagaagtaaa attattcttc ttctggctcg tcgtcaacgt
     7921 ccacttccgg agcgatttca tcgtcccctt ccgcggcact gccatcgatg gtatccagat
     7981 cttcctcgtc aaccggttca gcaacacgtt gcagacccac tacgttttca tcttccgcag
     8041 tacggatgag gatcacgccc tgggtgttac ggcctacgat gctgatttcc gaaacgcgag
     8101 tacgtaccag cgtaccggca tcggtgatca tcatgatctg gtcgcagtca tctacctgta
     8161 ccgcgccaac cactaaacca ttacgttcag taaccttaat ggagataacc cctttcgtcg
     8221 cacgcgactt ggttgggtat tccgccactg cggtacgttt accgtaaccg ttttgcgttg
     8281 cggtgaggat tgcgccatcg ccacgaggca cgatcagaga gacgacttta tcgccttcac
     8341 ctaagcgaat accgcgaaca ccggtggtgt tgcagcccat cgcacggaca gaagactctt
     8401 taaagcgcac cactttacct tcagcggaga acagcattac ttcatcttcg ccactggtca
     8461 ggtcaacgcc gatcagctca tcgccttcaa ccagtttgat cgccacttta ccggcggtac
     8521 gcagacggtt gaattcggtg aggacggttt tcttcacagt accgttagcg gtcgccatga
     8581 agactttcac gccttcttca aactcggtca ccggcaggat cgcggtgata cgttcgtcct
     8641 gctccagcgg cagcaggttg acgatcggac gaccgcgcgc gccacgagag gcttccggca
     8701 actgatagac tttcatcgaa tagacgcgac cacggctgga gaagcacagg atatggtcgt
     8761 gggtattcgc caccagcagt cggtcgataa agtcttcttc tttaatacgt gcggcagatt
     8821 tacctttccc gccacgacgc tgcgcttcgt attcagaaag cggctgatac ttaacgtagc
     8881 cctggtgaga tagtgtcact accacatctt cctgggtgat cagatcttcc aggttgatgt
     8941 ctgcgctgtt ggcggtgatt tcggtacgac gtttgtcacc gaactgttca cgaaccagct
     9001 ccagctcttc acggatcact tccatcagac gatcggcgct accaagaata cgcagcagtt
     9061 ctgcgatctg atccagcagc tctttgtatt cgtcgagcag tttttcgtgc tcaaggccgg
     9121 tcagtttctg caaacgcaga tccagaatcg cctgagcttg ctgttcggtc aggtagtaca
     9181 gaccatcacg cacgccgaac tctggctcca gccattccgg acgcgcagca tcgtcgccag
     9241 cacgttcgag catcgcggca acgttgccca gctgccacgg attagcaacc agcgcagttt
     9301 tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat gatcgggtcg atgttcgcca
     9361 gcgccacggc taatgcttca aggatatgag cacgatcgcg agctttacgc agttcgaaaa
     9421 tagtacgacg ggtcaccact tcacggcggt gacgaacaaa cgccgcgatg atgtctttca
     9481 ggttcatgat cttcggctga ccatggtgca atgccaccat gttgataccg aaagaaacct
     9541 gcaactgggt ctgggagtag aggttgttaa gcacaacttc accgaccgca tcgcgtttca
     9601 cttcaatcac gatgcgcata ccgtctttgt cagactcgtc acgcagcgcg ctgatgcctt
     9661 ccacgcgttt ttcttttacc agttccgcaa tcttctcgat caggcgcgct ttgtttacct
     9721 gatacggaat ttcgtggacg ataatggttt cacgtccggt tttggcgtca acttccactt
     9781 cagcacgggc gcggatatat accttgccgc gaccggtacg gtaagcttct tcaataccgc
     9841 gacgaccgtt aatgattgcc gccgtcggga agtccggccc cgggatgtgt tccatcagcc
     9901 cttcaatgct gatgtcttca tcatcgatat acgccagaca accgttgatg acttccgtca
     9961 ggttgtgcgg cgggatgttg gttgccatac ctacggcgat accggaagaa ccgttcacca
    10021 gcaggttagg aattttggtt ggcatgacgt ccggaatttt ttccgtgccg tcatagttat
    10081 caacgaaatc gaccgtctct ttttcgagat cggccatcag ttcatgggca attttcgcca
    10141 gacggatttc cgtataacgc attgccgccg cagagtcgcc gtcgatggaa ccgaagttac
    10201 cctgaccgtc taccagcatg taacgcagcg agaatggctg cgccatacgg acgatcgtgt
    10261 tataaaccgc caagtcacca tggggatggt atttaccgat tacgtcacca acgacacggg
    10321 cagatttttt ataggctttg ttccagtcat tgcctagtac gttcatggcg taaagtacgc
    10381 gacggtgtac cggcttcaga ccatctcgga catctggcag cgcacggcca acaatgaccg
    10441 acatcgcata atccagatag gagctcttca gctcttcctc aatgttgacc ggtgtaattt
    10501 ctctcgcaag gtcgctcatc taaccgctat ccctctactg tatcccggat tcaaaggtcg
    10561 caaattataa cacagccgcg cagtttgagg taaacctata cgctttattc acatccaatg
    10621 cctgatatac tcgtttgtct tgccaattac ggagtagaag tgccaatgaa tgccgaaaaa
    10681 tcgccggtaa accataacgt agaccacgaa gagatcgcta aatttgaagc cgtcgcctcc
    10741 cgctggtggg atcttgaagg tgagttcaaa ccgctgcacc gcattaaccc gctgcgtctg
    10801 ggctatattg ccgagcgtgc tggcggttta tttggcaaaa aggtgctcga tgtcggttgt
    10861 ggcggcggca ttctggccga gagtatggcg cgcgaaggcg cgacggtgac cggtctggat
    10921 atgggctttg agccattgca ggtggcaaag ctgcacgcac tggaaagcgg cattcaagtg
    10981 gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac atgccgggca gtatgatgtg
    11041 gtgacctgca tggagatgct ggagcacgtc cccgatccgc agtcagtggt cagagcctgt
    11101 gcgcaactgg tgaaaccggg cggcgatgtc tttttctcga cgcttaaccg caacggcaag
    11161 tcatggctga tggcggtggt tggcgcggaa catattttgc gcatggtgcc caaaggcacg
    11221 catgatgtga agaagtttat taaaccggca gagctgcttg gctgggtaga tcagacaagc
    11281 cttaaagagc gacatataac cgggctacat tacaacccga tcactaatac ttttaaactc
    11341 ggccccggcg tggatgtgaa ctatatgctg cacacgcaga ataagtgagg ttgatgtttg
    11401 gccgcgccaa tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa atgctccccg
    11461 taggccggat aaggcgttta cgccgcatcc ggcaaccgtg ccgactagac agtgattacc
    11521 atttcaccgt catcgacaaa aaccctgccg tctgggcaaa atcatcactc cctttctgcc
    11581 acgccacgct gccgcgcagg gacactcgct gactgatatt gcccgtgact cccactttta
    11641 tttcaccccg ttgcttcacc gcatcgtcac tgatagtgct gccatcctct tcaatttccg
    11701 tcgaatgggg atcgtgataa taattcagat ctaatgttgg tatgacatga acagcggtac
    11761 gccattcgct gtgtaaaccc agccgcgtct gaatatcatc accctgcgat tgtgacacgc
    11821 gcgcacggtt agcggcggta aaatcatcct gctgcacgcc ctgataaatc acctgcgcct
    11881 gcggttcaat caccacacca cgccccggta accactgata ccccgcctcc agcgaggcga
    11941 taatccccga cgagtggtaa tgatctgtgc catcttcttg ttcggaaaca tcattgctaa
    12001 accacgcgta ttgcagccag ctatccagcc aggccccttg cttctgatta ccgtgctgaa
    12061 accagcttga tgtcagccca acggcataac cgtggttctg gttatcggcg cgagttccgg
    12121 tcatattcga gcggctgtcg ccctggttat cgctgtagcc accaacaatc ccaagcatcc
    12181 actcgccatc cgtgccccag cgcccgctaa acagatcgcc gctaagttgc accgtagaag
    12241 tgtcttcatg ttgagccagt tgccccgctg ctgtgtaatg ataatctccg ccgataacac
    12301 gtaaattcag cgtctgacca tcgccacctg cgtgatcgcg tcgctccatc ataaacgcct
    12361 gatttgccgc ccgcaggtta ttgagataac cgccaacttt ggcattcaac accggctggt
    12421 aagcaggcgt aggttccggg tcgggtgttg gatcaggatc cggcgtggga tcaggatcgg
    12481 gagtcgggtc tggatcaggt ggcgatggcg gcgttacttc ttgcgatcgc agataccagt
    12541 cgttgttatc ttccaccagc gtgtagtcat acgctcccat attgacgtag ccgctgcctg
    12601 ccagactgaa ctgcgcattg ttttgaaact gcgtgggatc agctgcgaaa tcaaccactt
    12661 taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt
    12721 tgccagcagt attaccgttc atcaccaatt gatcgcttac cgagtcatcg ccgtttaatt
    12781 cgctatcgag cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc
    12841 cagcgacgcc attttgcagg ctaaccgcac cggacgtatt ggtcagattg ccattaaccg
    12901 tcacgttgga aagataagtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca
    12961 acatcacacc atcggtggca ttcaccagcg tcccatcgcc gttaaatagt gaggcattaa
    13021 gggcgacgga agtatcctgc ccggtcaggc gcaaaaccgt accatcgctg atgtcgatag
    13081 tgccgctgga aatagcatca attgactgaa tatcctgatc cgcgccagta acgaacgttg
    13141 ccccatcacc aacccacagt gacgaagcat aaggaaggat atcagcaaca tcacctttca
    13201 gggtgccttc ttcgacacgt accgccgact gcgttgtacc gctggcggtc agctccagtg
    13261 taccagcccc cgttttagtc aatgtgctac cctcatcctg atgctgcccg ctgctgtcag
    13321 ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc
    13381 gcatttcaat atcacgtccg tggcctgccg catctgaacg cgtgtcaacg tcggaggtaa
    13441 tctccatcac gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat
    13501 ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga ggcgatttgt gttgtgccca
    13561 aataactatt attgtttgcc agacttacct gcccgtcgct cgcatcgacc accacgtaga
    13621 cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc acctgcaaga tctaccacat
    13681 cctgactgcc gataaccgtt ccgctgctgc cactgacttc aaggccatcg ttgtatgatg
    13741 tgccgctctg ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg
    13801 actgcggatc gtcctggaga gcggtaagat ctgccgcgtc gccttccagc gaaagcaccg
    13861 caccatcatc aacgactata tcgccggtta gcgccatcga ctgcgcccct gccagcacgt
    13921 agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc gatgatgcca gcaaaactgc
    13981 cctgattaac agtaacgttg ccaccagcat cgatatttaa agtgccattc tgaaagcccg
    14041 tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt tagctcagcc tgattctgat
    14101 actgatcaat actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg
    14161 tatcgccgac attcatcagg gagttgctgc ggcccagggt aacttcaccg ttttcaatct
    14221 gcatctcacc agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt
    14281 tggtgattaa cccagtacca gcaatagagt caacagctcc gtcattctct gtattgccaa
    14341 taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa
    14401 agccacccgc cgcagaggaa ggaccatctc catagcctgc tgcgctattg ttctcatcga
    14461 ctaacacgcc tccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag
    14521 ccgtcacgct attggtatat atcgccccgc cataaccttc ggcagtgtta tttgtaaagg
    14581 cagtgttatt tactatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa
    14641 catcgattgc cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat
    14701 caaaaataac atcacttaaa taaacgtcat tattaatggt ataaattgcg ccacctttgc
    14761 catcattagc gatgttattg cgaaacatgg cgttagtgac acgtaaatcg acggcaccag
    14821 tatcgttagt accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccggaaa
    14881 aaataacatc agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat
    14941 tatattctcc tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta
    15001 atagtgaaaa ttccgctcct tgttgcaaaa atacggcccc accgctggcg ttattggtca
    15061 tatccgaaaa aaccagccat tgcccatcag cgatactcca gtcctgcgta atgcctgaaa
    15121 ggctttgcct gctggcctga caactcgctt tgacatcata tccctggcat gaatcagtga
    15181 ccgccgcgac accgttagca gagaaaagag atgcaatcat tgacgggagt aaagataaat
    15241 actccttgcg tagaaagata atccgcatat taatctaacc atcattttct ataagacggc
    15301 gtatttaatc gcattatacg atatggaata tctttctttt gtaaattgtt caacccggtg
    15361 caatggctat tttgttatta tttgatgaat aatatcagtg cgtcataatt caagttaata
    15421 accttcaggg atatcagtta tatttaaact aaattaaagt catgaataat tttcttataa
    15481 tataaggtaa attaacaaaa tggcttagca tttaacaata accgaataga aaacaaccat
    15541 ttcgccatca acaatctctt acattcgctt atatattgac cacaactgat acatcagatt
    15601 atgtgatgac tcgtgcttag atcaattttt gcaatcatta gcaaaaagat taataaacca
    15661 tctatatcaa tttatctaac ctattatgcc gttcaagaaa tcgccgaaca gttattttta
    15721 acaaattttt ctcttcccat tgactttccc ggacaccttg tctgacctaa ggtgcgcgaa
    15781 agccactttt tccttcctga gttatccaca aagttatgca cttgcaagag ggtcattttc
    15841 acactatctt gcagtgaatc ccaaacatac cccctatata tagtgttcta agcagcttcc
    15901 cgtactacag gtagtctgca tgaaactatt gcggaaagaa ttccaaaaac aggtacgaca
    15961 tacatgaatc agaatctgct ggtgacaaag cgcgacggta gcacagagcg catcaatctc
    16021 gacaaaatcc atcgcgttct ggattgggcg gcagaaggac tgcataacgt ttcgatttcc
    16081 caggtcgagc tgcgctccca cattcagttt tatgacggta tcaagacctc tgacatccac
    16141 gaaaccatta tcaaggctgc cgcagacctg atctcccgtg atgcgccgga ttatcagtat
    16201 ctcgccgcgc gcctggcgat cttccacctg cgtaaaaaag cctacggcca gtttgagccg
    16261 cctgcgctgt acgaccacgt ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg
    16321 ctggaagact acacggaaga agagttcaag cagatggaca cctttatcga tcacgaccgt
    16381 gatatgacct tctcttatgc tgccgttaag cagctggaag gcaaatatct ggtacagaac
    16441 cgcgtgaccg gcgaaatcta tgagagcgcc cagttccttt atattctagt tgccgcgtgc
    16501 ttgttctcga actacccgcg tgaaacgcgc ctgcaatatg tgaagcgttt ttacgacgcg
    16561 gtttccacat ttaaaatttc gctgccgacg ccaatcatgt ccggcgtgcg taccccgact
    16621 cgtcagttca gctcctgcgt actgatcgag tgcggtgaca gcctggattc catcaacgcc
    16681 acctccagcg cgattgttaa atacgtttcc cagcgtgccg ggatcggcat caacgccggg
    16741 cgtattcgtg cgctgggtag cccgattcgc ggtggtgaag cgttccatac cggctgcatt
    16801 ccgttctaca aacatttcca gacagcggtg aaatcctgct ctcagggcgg tgtgcgcggc
    16861 ggtgcggcaa cgctgttcta cccgatgtgg catctggaag tggaaagcct gctggtgttg
    16921 aaaaacaacc gtggtgtgga aggcaaccgc gtgcgtcata tggactacgg ggtacaaatc
    16981 aacaaactga tgtatacccg tctgctgaaa ggtgaagata tcaccctgtt cagcccgtcc
    17041 gacgtaccgg ggctgtacga cgcgttcttc gccgatcagg aagagtttga acgtctgtat
    17101 accaaatatg agaaagacga cagcatccgc aagcagcgtg tgaaagccgt tgagctgttc
    17161 tcgctgatga tgcaggaacg tgcgtctacc ggtcgtatct atattcagaa cgttgaccac
    17221 tgcaataccc atagcccgtt tgatccggcc atcgcgccag tgcgtcagtc taacctgtgc
    17281 ctggagatag ccctgccgac caaaccgctg aacgacgtca acgacgagaa cggtgaaatc
    17341 gcgctgtgta cgctgtctgc tttcaacctg ggcgcaatta ataacctgga tgaactggaa
    17401 gagctggcaa ttctggcggt tcgtgcactt gacgcgctgc tggattatca ggattacccg
    17461 atcccggccg ccaaacgtgg agcgatgggt cgtcgtacgc tgggtattgg tgtgatcaac
    17521 ttcgcttact acctggcgaa gcacggtaaa cgctactccg acggcagcgc caacaacctg
    17581 acgcataaaa ccttcgaagc cattcagtat tacctgctga aagcctctaa tgagctggcg
    17641 aaagagcaag gcgcgtgccc gtggtttaac gaaaccactt acgcgaaagg gatcctgccg
    17701 atcgatacct ataagaaaga tctggatacc atcgctaatg agccgctgca ttacgactgg
    17761 gaagctctgc gtgagtcaat caaaacgcac ggtctgcgta actccacgct ttctgctctg
    17821 atgccgtccg agacttcttc gcagatctct aacgccacta acggtattga accgccgcgc
    17881 ggttacgtca gcatcaaagc gtcgaaagac ggtattttgc gccaggtggt gccggactac
    17941 gagcacctgc acgacgccta tgagctgctg tgggaaatgc cgggtaacga tggttatctg
    18001 caactggtgg gtatcatgca gaaatttatc gatcagtcga tctctgccaa caccaactac
    18061 gatccgtcac gcttcccgtc aggaaaagtg ccgatgcagc agttgctgaa agacctgctc
    18121 accgcctaca aattcggggt caaaacactg tattatcaga acacccgtga cggcgctgaa
    18181 gacgcacaag acgatctggt gccgtcaatc caggacgatg gctgcgaaag cggcgcatgt
    18241 aagatctgat attgagatgc cggatgcggc gtaaacgcct tatccggcct acggct
//
LOCUS       DACAPB010000085        18289 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.085, whole genome shotgun sequence.
ACCESSION   DACAPB010000085 DACAPB010000000
VERSION     DACAPB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.085"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(68..889)
                     /gene="bacA"
                     /locus_tag="IT904_002579"
     CDS             complement(68..889)
                     /gene="bacA"
                     /locus_tag="IT904_002579"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP3192346.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(980..1348)
                     /gene="folB"
                     /locus_tag="IT904_002580"
     CDS             complement(980..1348)
                     /gene="folB"
                     /locus_tag="IT904_002580"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAP3192347.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1453..2070
                     /gene="plsY"
                     /locus_tag="IT904_002581"
     CDS             1453..2070
                     /gene="plsY"
                     /locus_tag="IT904_002581"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP3192348.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2083..3015)
                     /gene="ttdR"
                     /locus_tag="IT904_002582"
                     /gene_synonym="dan"
     CDS             complement(2083..3015)
                     /gene="ttdR"
                     /locus_tag="IT904_002582"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAP3192349.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3222..4133
                     /gene="ttdA"
                     /locus_tag="IT904_002583"
     CDS             3222..4133
                     /gene="ttdA"
                     /locus_tag="IT904_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAP3192350.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4130..4735
                     /gene="ttdB"
                     /locus_tag="IT904_002584"
     CDS             4130..4735
                     /gene="ttdB"
                     /locus_tag="IT904_002584"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAP3192351.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4783..6246
                     /locus_tag="IT904_002585"
     CDS             4783..6246
                     /locus_tag="IT904_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAP3192352.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDASMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6289..7302)
                     /gene="tsaD"
                     /locus_tag="IT904_002586"
     CDS             complement(6289..7302)
                     /gene="tsaD"
                     /locus_tag="IT904_002586"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP3192353.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7540..7755
                     /gene="rpsU"
                     /locus_tag="IT904_002587"
     CDS             7540..7755
                     /gene="rpsU"
                     /locus_tag="IT904_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP3192354.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7866..9611
                     /gene="dnaG"
                     /locus_tag="IT904_002588"
     CDS             7866..9611
                     /gene="dnaG"
                     /locus_tag="IT904_002588"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP3192355.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9806..11647
                     /gene="rpoD"
                     /locus_tag="IT904_002589"
     CDS             9806..11647
                     /gene="rpoD"
                     /locus_tag="IT904_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP3192356.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMGEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11726..12232)
                     /gene="mug"
                     /locus_tag="IT904_002590"
     CDS             complement(11726..12232)
                     /gene="mug"
                     /locus_tag="IT904_002590"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAP3192357.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12357..12432
                     /locus_tag="IT904_002591"
     tRNA            12357..12432
                     /locus_tag="IT904_002591"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12390..12392,aa:Ile,seq:cat)
     gene            complement(12486..13250)
                     /gene="nfeF"
                     /locus_tag="IT904_002592"
     CDS             complement(12486..13250)
                     /gene="nfeF"
                     /locus_tag="IT904_002592"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAP3192358.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13538..14161
                     /locus_tag="IT904_002593"
     CDS             13538..14161
                     /locus_tag="IT904_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP3192359.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14315..15835)
                     /locus_tag="IT904_002594"
     CDS             complement(14315..15835)
                     /locus_tag="IT904_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP3192360.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVAHQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16142..17632
                     /gene="ygjG"
                     /locus_tag="IT904_002595"
     CDS             16142..17632
                     /gene="ygjG"
                     /locus_tag="IT904_002595"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAP3192361.1"
                     /translation="MITEFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(17674..18006)
                     /locus_tag="IT904_002596"
     CDS             complement(17674..18006)
                     /locus_tag="IT904_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAP3192362.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGQKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            18225..>18289
                     /locus_tag="IT904_002597"
     CDS             18225..>18289
                     /locus_tag="IT904_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP3192363.1"
                     /translation="MATLKDIAIEAGVSLATVSRV"
BASE COUNT         4442 a         4986 c         4648 g         4213 t
ORIGIN      
        1 cggcgtgaac gccttattcg gcctacgcaa tcgttgacaa cgccaagcat ccgacactat
       61 tcctcaatta aaagaacacg acatacaccg cagccgccac aataaagcga taaatggcga
      121 acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt tttaatcgct atcagcgcca
      181 ccacaaaagc ggtgataaac ccaacggcaa acatcgggat atcgccgctt gtcaggaagc
      241 cccagctttt gtagagatcg agcgccgttg cgcccatcat catcggcacc gccagcagga
      301 acgaaaactc ggaagcagcg taacggctca cccccatcag catcccacct gaaatggtcg
      361 ccccggaacg ggagaaaccc ggccacagcg ccagacactg gaaacagcca atcataaacg
      421 cctgacgata ggtcatatca tcaagacccg gcgcacgcgg ctctttcggc ttcaggcatt
      481 cggcggcaat cagcaacaaa ccgccaacga ccagcgcata catcacattt atcgggttaa
      541 acaatgactt aatcgtgtcg tggaacaata gccccagtac caccgccgga atcatcccca
      601 gcaaaatgtg gatcagcgtt aaacgacctt tgctttcacc ttcgtgctgc aacgggcggc
      661 caaagtggat gccaatcagg ccaaacagac gccgccagaa catcactact accgccagaa
      721 ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt gtcgccctca aaccccaaca
      781 agtgaccgac aataatcata tggcccgtgc tggataccgg cagaaattct gtcaatcctt
      841 cgaccacacc caatattgcc gctatcagca gcgagtgcat atcgctcatc aataaacccc
      901 taaattatta aaatgtaccg cttgtccgaa ctactgcagt atgaccaggt tataaccgtt
      961 tggtttaaca gctgtaaaat taattatttt ctttcagatt attgccacgc tcaatgatta
     1021 cgccaacatt cgccgcccgc gccactgcgc ctggcttgct gagtttgata cgcacccacg
     1081 gcgagttgaa gcgtgctaac agcagctccg ccacctcttc agccacgcgt tccaccagcg
     1141 caaaacgcgc cccctcgacg tggctgacca ccgtttctgc aatgtcagcg taactgaggc
     1201 aatccgccac atcatcactt ttcgccgctt tacggttatc ccacgccatt tcgatatcga
     1261 acactaactt ctgttcgatg gtctgttccc agtcgtaaac accaatagtg gtgattaccg
     1321 aaagttgctc tataaataca atatccatca cgtcctgcct gctttttggc taaccggata
     1381 ccacttccgg cgaaatgtgc gtattatcca cagattcatc gttgaacacg aattttcaaa
     1441 acggaacagc ttatgagtgc aatcgcgcct ggaatgatcc tcatcgcgta cctctgcggc
     1501 tccatttcca gtgccattct ggtttgccgc ttgtgtgggc tgcccgatcc gcgaaccagc
     1561 ggctccggta atcctggcgc aaccaatgtg ttacgtatcg gtggcaaggg agcagccgta
     1621 gcagtactga ttttcgacgt tctgaaagga atgttgcccg tctggggcgc gtatgaatta
     1681 ggtgtcagcc ccttctggct aggcttaatt gccatcgccg cctgtcttgg acacatctgg
     1741 cccgttttct tcggttttaa aggaggaaaa ggcgttgcta ccgcttttgg tgccatcgca
     1801 ccaattggct gggatctcac cggagtaatg gcgggaacct ggttactgac cgtgctattg
     1861 agcggatact cgtcgctggg agcgattgtc agtgcactga ttgctccgtt ttatgtctgg
     1921 tggtttaagc cacaattcac cttcccggtt tcgatgctct cttgcctgat cctgctgcgt
     1981 catcatgaca acatccagcg tctgtggcgt cgtcaggaga caaaaatctg gacgaaattc
     2041 aaaagaaagc gcgaaaaaga tcccgagtga tttctggtgg atctacataa cctgatagcc
     2101 ttcgtcgggc ttgcccagcc gttgctggca ccatgccgcc agaaattcca cgcagacacg
     2161 taatttcatg ctgcgataga gcggctcccg gtaaacagcc cagatattgg cgctttgtgc
     2221 atactctggc aatacttgca ccagtttgcc actctccaga aacggcaaca catcccactc
     2281 ggaacgcagc ataatccctt tgccctccag cgcccattgc agcacaatct cgccgctatt
     2341 ggaggaaaga tgcccgctta ccttcaccga ttttttctcc tgaccgttcc ccaactccca
     2401 tattccatgg gtcatatcgc gctctttggt caccagacag tcatgacgac ttaattcctg
     2461 tagggattgt ggctgcggat atttttgcag atactcaggt gctgcgcata atattctttt
     2521 attttttgtc aaaagatgcg caatataata atcaggaatt tcgtcattaa tacgaatatc
     2581 cagatcaata ttatcctgca ctaaatcaat ttgccgatcg aacaattcaa aatgcacctg
     2641 taactcagga taattgcgca tcagttcggt aatagctggc gcaatatggc tgcgcccaaa
     2701 accaaaactg caaccaatac gaatcatccc ctccgggcgc gttttgattt gcgtgacgtc
     2761 atcgaccagt cgctgatatt gggtgaggat ttccaacgca tgttcgtaac aacgttggcc
     2821 actttcagtc agcgccacgc cgcgagcgga gcggttcagc agcgttgttg ccagcgtatt
     2881 ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtc tgcccaagcg tcgccgctgc
     2941 ggcgctaaaa ctcccgctgt gaacgatttc taccagtacc tgcaagtctt tggctaaagg
     3001 ccagctattc agcatcgtta attatccgca gttgtgataa gcgcagtgta tttcgcaaaa
     3061 cattgccatt ccctgctctg gcgcaatatt cattcttatt ttcgcgttga ttcacgaact
     3121 gttaattaca tattcacggt ggcaaaaaat ataaaaccac atttttagtg gtagtttgtg
     3181 gcggtgaatt tttccagaca aatacaaaaa ctggagttgc catgatgagc gaaagtaata
     3241 agcaacaggc agtgaataag ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta
     3301 cccgaatgcc tgatgacgtg gtggataaac taaaacagct aaaggatgcc gaaacgtcgt
     3361 cgatggggaa aattatctac catacgatgt tcgacaacat gcaaaaagcg attgacctga
     3421 atcgtcctgc ctgtcaggac accggggaga ttatgttctt cgttaaagtc ggttcccgct
     3481 tcccactgct tggcgagctg caaagcatac tcaaacaagc cgtggaagag gcaaccgtca
     3541 aagcgccact acgtcacaat gcggtagaaa tttttgacga agtaaacacc ggcaaaaata
     3601 ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc cgacaatgac gatgcggaaa
     3661 tcgaagttta catggcaggc ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc
     3721 cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga aaatatctcc accctcgccg
     3781 taaacgcctg tccaccggta ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg
     3841 ccgtactctc gcgtaaagcc attttgcgcc cgattggcag ccgccatccc aatccaaaag
     3901 cggcagaact ggagctacgc ctggaagaag gactcaaccg tctggggatt ggtccacaag
     3961 ggctgaccgg caacagttca gtgatgggcg tacatatcga atctgccgcc cgccatccgt
     4021 caaccatcgg cgttgctgtc tctaccggct gctgggcgca tcgtcgcggc acgctgctgg
     4081 ttcatgccga tctcaccttt gaaaatctgt ctcacacccg gagcgcgtta tgaaaaagat
     4141 cctgacaacc ccgatcaaag ctgaagatct gcaagatatt cgcgtcggcg atgtgatcta
     4201 cctgaccggt acgctggtga cctgccgcga cgtttgtcac cgccgtttga tcgaactgaa
     4261 acgtccgatc ccttacgatc tcaacggcaa agcgattttc cacgctggcc ccatcgtgcg
     4321 caaaaacggc gacaaatggg agatggtctc cgtcggcccg acaaccagta tgcgtatgga
     4381 aagttttgaa cgtgaattta ttgagcagac cggcgtgaaa ctggtggttg gcaaaggtgg
     4441 tatggggccg ctgaccgaag aaggctgcca gaaattcaag gcgctacatg tgattttccc
     4501 ggcaggctgc gcggtgctgg cggcaaccca ggtggaagag attgaagaag tgcactggac
     4561 agagctcgga atgccggagt cactgtgggt ctgccgggtc aaagagttcg gcccgctgat
     4621 tgtctctatt gatacccacg gcaacaacct gatagccgaa aacaaaaagc tgttcgccga
     4681 acgccgcgat cccatcgtgg aagagatctg cgagcacgtc cattacatca aataaccctc
     4741 ccggagaggc tcacccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
     4801 tggtggcgat atcttgcgcc gctggcggtc atcgccatta ttgctctact tccggttccc
     4861 gcagggctgg agaaccatac ctggctctac tttgccgttt ttactggcgt gatcgttgga
     4921 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatatc catcatcgcc
     4981 atactctctc cctggctgct gttcagcccg gagcagctcg ctcagccagg ctttaaattc
     5041 actgcaaaat ccctctcgtg ggccgtttcc ggtttttcta attcggttat ctggctgatt
     5101 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg
     5161 ctgattctgg tgaaaaagat gggacatcgc acgctgtttc tcggctatgc ggtgatgttc
     5221 tccgagctga tcctcgcacc tgtaacaccg tccaactcgg cgcgtggtgc ggggattatc
     5281 tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg
     5341 cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
     5401 attttcttga cggcgatggc accaaacttg ctgttaattg gactgatgaa aagcgcatct
     5461 cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag cattttactg
     5521 gttctgctgg ttccctggct ggcttacgtg ctgtacccgc cggtactgaa gtctggcgat
     5581 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
     5641 aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat
     5701 atcgatgcct cgatggtcgg ttacagcgtg gtggcactga tgctgcttct gcgcattatc
     5761 agttgggacg acattgtcag taataaagcg gcgtggaacg ttttcttctg gctggcctcg
     5821 cttatcaccc tcgccaccgg actcaacaac accggtttta ttagctggtt tggcaaactg
     5881 ttagcaggca gcttaagcgg ttattcgcca acgatggtga tggtggcgtt gattgtggtg
     5941 ttttatctac tgcgctactt tttcgccagc gccacggcgt atacctccgc tctcgcaccg
     6001 atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
     6061 gttggtgcgg caattggtct ggggagcatt cttacaccat acgccaccgg ccccagtccg
     6121 atttactacg gcagtggtta tctgccaacg gcggattact ggcgactggg ggcgattttt
     6181 gggctgatat tcctcgtatt gctggtgatt accggcttac tgtggatgcc cgtggtgttg
     6241 ctttaagtga ggacggtgcc ggaaattgct ctccggcacc caatagtttt acgcagccgg
     6301 taactccgcc agcggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc
     6361 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
     6421 acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tcagccagct tcgcccgtaa
     6481 cgtacggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccag
     6541 cgcccgcttg cacttaatca tcagcgtatc gaccaccgca tcttcaaagg cgcgggcgat
     6601 atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatg gtatttgccg cgaaggtttt
     6661 caggccggag aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaagacaaa
     6721 gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggcccgccag gataatccag
     6781 ccccagcagc ttcgcggttt tatcaaacgc ttccccggcg gcatcatcga tagactcgcc
     6841 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggaaac
     6901 aagcagcgca acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg
     6961 cccttccata tggtgtacag ggatcgccgg aacgtcccag gcaaacgcca gagaacgccc
     7021 cacggtcgcg ccaaccagta gcgcgccgac taatccaggg cctgcggtat aggccacagc
     7081 atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca acggtacggt
     7141 tttacgcaca tgatcgcggg aggccagttc aggcacgacg ccgccgtagt cagcgtgcaa
     7201 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
     7261 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
     7321 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
     7381 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg ccgcggtgct
     7441 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
     7501 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
     7561 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
     7621 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
     7681 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
     7741 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
     7801 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
     7861 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca cgcactgaca
     7921 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
     7981 gttgtccatt ccacaacgag aaaaccccgt ccttcaccgt taacggtgag aaacagtttt
     8041 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
     8101 agctcgagtt cgtcgaaacg gtcgaagagc tggcagcaat gcacaatctt gaagtgccat
     8161 ttgaagcagg cagcggcccc agccagatcg agcgccatca gaggcaaacg ctttatcagt
     8221 tgatggacgg tctgaatacg ttttaccaac aatctttaca acaacctgtt gccacgtctg
     8281 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgctcgc tttgcgattg
     8341 gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
     8401 gccagtcatt gattgatgcg gggatgttgg tcactaacga tcagggacgc agttacgatc
     8461 gtttccgcga gcgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
     8521 gcgggcgcgt gctgggcaac gataccccca aatacctgaa ctcgccggaa acagacattt
     8581 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
     8641 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
     8701 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
     8761 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgc gatgccgcct
     8821 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg ccgtcagcta cgctttatgt
     8881 ttttgcctga tggcgaagac cctgacacgc tagtacgaaa agaaggtaaa gaagcgtttg
     8941 aagcgcggat ggagcaggcg atgccactct ccgcatttct gtttaacagt ctgatgccgc
     9001 aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca ctaccattaa
     9061 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
     9121 taggcatact tgatgacagc cagcttgaac gattaatgcc aaaagcggca gagagcggcg
     9181 tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
     9241 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
     9301 tccctggact tggcttattc agagaactgg tcaacacttg tctctcccag ccaggtctga
     9361 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
     9421 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
     9481 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
     9541 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
     9601 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
     9661 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
     9721 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
     9781 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
     9841 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
     9901 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
     9961 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
    10021 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
    10081 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
    10141 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
    10201 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
    10261 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
    10321 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
    10381 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
    10441 cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc gctcagtacg ttgtaacgcg
    10501 tgacaccatc aaagcgaaag gtcgcagtca cgctaccgct caggaagaga tcctgaaact
    10561 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
    10621 catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
    10681 tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca acgaaaccag
    10741 cgatacctgg ttcaacgcgg caattgcgat gaacaagccg tggtcggaaa aactgcacga
    10801 tgtctctgaa gaagtgcatc gcgccctgca aaaactgcag cagattgaag aagaaaccgg
    10861 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
    10921 ccgccgtgcg aagaaagaga tgggtgaagc gaacttacgt ctggttattt ctatcgctaa
    10981 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggca acatcggtct
    11041 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
    11101 ctggtggatc cgtcaggcga tcacccgctc tatcgcggat caggcgcgca ccatccgtat
    11161 tccggtgcat atgattgaga ccatcaacaa gctcaaccgt atttctcgcc agatgctgca
    11221 agagatgggc cgtgaaccga cgccggaaga actggctgaa cgtatgctga tgccggaaga
    11281 caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa cgccgatcgg
    11341 tgatgatgaa gattcgcatc tgggggattt catcgaggat accaccctcg agctgccgct
    11401 ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac gacgtgctgg ctggcctgac
    11461 cgcgcgtgaa gcaaaagttc tgcgtatgcg tttcggtatc gatatgaaca ccgaccacac
    11521 gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa cgtatccgtc agatcgaagc
    11581 gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa gtgctgcgta gcttcctgga
    11641 cgattaatcg gtaggccgga tcaggcgtta cgccgcaccc ggcactaggc cctctgcaca
    11701 aacgccacct tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct
    11761 cgcgatacgc ttcaaccagt ttctccagtg aaacgcgact taaaccgctg ggatttggca
    11821 gcacccaaat ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct
    11881 ggctgaatcc ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat
    11941 cttcaatttt ttcaatcagc ttacgcccgc ctgcgtgtag ctcctgcttt gaaacttcat
    12001 tggcttgcac cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat
    12061 gctgtgcctc ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc
    12121 agaagcgatt tgccggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt
    12181 tgataccgca aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc
    12241 ctttctaaca tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc
    12301 aggcgcgcgg taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc
    12361 ccttagctca gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag
    12421 caggggccac cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat
    12481 atcagttact ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc
    12541 gcttcaaagc ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag
    12601 taatcatcgg cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt
    12661 gccagccatt cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc
    12721 cgcacgctaa ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccagg
    12781 cggcggcgca atgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct
    12841 tccggcacca ccagcgaacc gcgcggacct gccaccgtaa gtttatcgcc cggttgcgcc
    12901 tgcatcgccc agccgctggc gaccccaccg tcgtgaataa agaaatcaat cgccagttca
    12961 tggcgtagtt cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc
    13021 cagacgatgc cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag
    13081 aagagtttgc tgtgatcgtc aaagccacgc gatgtaaaac cgtccagcgc ctcgccgccg
    13141 aggacaatgc gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg
    13201 aagcgcagat cattgcgaac gcgctgcggg tagcgggggg tgttattcat tgttatcgcc
    13261 ttcgtgatgg taatcagata tatctaaata aaactcgcaa atgataatga ttgttaatca
    13321 tgataaatgc aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct
    13381 cttctcaggc atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg
    13441 tgagatgagt ccacgtttaa aaatcatttt tacacttgca agaacgctca tatcagatat
    13501 aaattagata tatctaatta agcaaaagga ggctgatatg agccatcatc acgaagggtg
    13561 ttgtaaacat gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca
    13621 cgagcactgc ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg
    13681 tcgcggcggc ggtcgtcggc aacgtttctt tggtcacggt gaattacgtc tggtgattct
    13741 ggatattctc tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct
    13801 aacccagggg aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca
    13861 ggagcagtcg ctgattacca tccgcgaaga ggaaggaggt aagaagcaga ttgcgctgac
    13921 cgaacaagga gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg
    13981 catcaaagcg cgttgcgttg gcgcggcgct gcgccagaac ccgcaaatga agcgggcgct
    14041 ggataatttt aaagcggtgc tggatttacg cgtcaaccag agcgatatca gtgatgcaca
    14101 aataaaaaag atcattgcgg tgatcgaccg cgccgctttt gatattacgc aactggatta
    14161 atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatg cgacgcttga cgcgccttat
    14221 ccggcctaca cccgctacac accccgcagg cctgataaga tgcgccagca tcgcatcagg
    14281 cattgtgctc caaccgccgg atccggcata ccgattaatg cagcaccgtc accgcgtctt
    14341 ccagtcggct ggcgcggtgt ttcaccatcg ccgacacctg cgcactctct tccaccagct
    14401 cggcattttt ctgggtgatc aggttaagct catccactgc acgggtcagg ctggaaagcc
    14461 catcggcctg ttccagcgtt gaatggctaa tctgggcgat caactgggtg acgtttttca
    14521 cctgtgccac aatatcttcc atcgtccgtc cggcggcgtg tacctgctgc gaaccggatt
    14581 gcaccttatc agcactggca tcaatcagct tgcgaatatc gttggcagca ttagcgctgc
    14641 ggctggctaa atgacgcact tcccctgcca ccactgcaaa acctttgccc tgttcgccgg
    14701 cacgcgccgc ttccaccgcc gcattcaggg ccagaatatt ggtctgaaac gcaatatcgt
    14761 taatcagcga agtaatggtg ccaatgcgct gggtactgtc ggcgatatcg tccattgtct
    14821 tgatcaccgt ggtcatcgcc tccccaccct gcactgccgc attactggca gtgattgaca
    14881 gtttatcggc agccgacgcc gtggcagagt tctgtttcac cgacgccgcc atttggttca
    14941 tggtcgccac cgtttgctga acgttatcaa ctgtctgctg ggtatgttcg ttcagttcat
    15001 cggtgccttt cgccagcgtc tcactgccat ttctgacact ggacacctgg cttgagacat
    15061 cgttaattag ccaacggcac atcaggccaa gttgccctac cgcacgtaat gtcagcccca
    15121 gctcatcgct gcgattcaga tgctcaacac tattacgttc tccggtcgcc accttcagtg
    15181 cctgatgggc aacattttct atcgggcgca caatctgcca ttcaaaacag gcgcttgcca
    15241 acaataccac taacgcacag aggatatacg tcaccaccgg ggcagcaaca aaccaaagca
    15301 tggccgccag caagataaac atcagggtca tcactccacg cgcccgccag cgaagcggta
    15361 atgaaggcag tttacccagc cagcctttac gcaccaccag gcctttatga atacgcttac
    15421 tggtacgtcc ggcgttcaac gctttgtaca gcggctccac cgccgcgatc tcttcatccg
    15481 tcgcccgggt acgaatcgac atatagccac tgatttttcc ctcgcgcacc atcggtaccg
    15541 cattggcccg cacccaataa tggtcaccat ttttgcggcg atttttcacg atgccgctcc
    15601 agggctcccc ttttttcagg gtgaaccaca tatccgcaaa cgccgctttt ggcatatccg
    15661 ggtgacgcac catgttgtgc ggctgccctt gtaactcttg caaggtatag ccgctcacct
    15721 gcacaaaagt gtcattagca tgagtgatat agctttgcag atcggtagtg gacatcagag
    15781 tggtatcgtc cgccagcggg gtattttgct gggtgacata cggatgagaa gacatgttcg
    15841 cgtcctgtgc aggttatatg gttgttaact tcttgtcaga gtttatgtcg gccccgctgc
    15901 ggttatcttt aaccgattaa tttgatttag atcgcaattt gcgatttaaa cacaaatcta
    15961 attccttgat ttaaaatact ttcactctgt tactatacga aaacgttaat tatcttgccc
    16021 aaaaatcagg caattattgc cctgaaaacg tgcatttgcg cagcaatcat caaatccata
    16081 cccgacaaaa accgtgcaaa ataacaataa atgttaacag atagcattaa atattgcaca
    16141 aatgataacc gaatttgtgt ttatcccgat tttcgcgatc gcagccggag tggcgcaatc
    16201 cctgcaatac ttaaatcggt atcatgtgat acgcgagcct ccggagcata ttttgaacag
    16261 gttaccttcg agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc tcattgagaa
    16321 gcgaacgctg gatcatgagg agatgaaagc acttaaccga gaggtgattg aatacttcaa
    16381 agagcatgtc aatccggggt ttttagagta tcgcaaatct gttaccgccg gcggggatta
    16441 cggagccgta gagtggcaag cgggaagttt aaatacgctt gtcgacaccc agggccagga
    16501 gtttatcgac tgcctgggag gttttggaat tttcaacgtg gggcaccgta atcctgttgt
    16561 ggtttccgcc gtacagaatc aacttgcgaa acaaccgctg cacagccagg agctgctcga
    16621 tccgttacgg gcgatgttgg cgaaaaccct tgctgcgcta acgcccggta aactgaaata
    16681 cagcttcttc tgtaatagcg gcaccgagtc cgttgaagca gcgctgaagc tggcgaaagc
    16741 ttaccagtca ccgcgcggca agtttacttt tattgccacc agcggcgcgt tccacggtaa
    16801 atcacttggc gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt ttatgccgtt
    16861 actgccgggc ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca cggctcttaa
    16921 cgagtgcaaa aaaaccggtg atgatgtggc tgcggtgatc ctcgaaccga ttcagggtga
    16981 aggtggcgta attctgccgc cgccgggcta tctcaccgcc gtacgtaagc tatgcgatga
    17041 gttcggcgca ctgatgatcc tcgatgaagt acaaacgggc atggggcgca cgggcaagat
    17101 gttcgcctgc gagcatgaga acgtacagcc ggatatcctc tgccttgcca aagcgctcgg
    17161 cggcggcgtg atgccgattg gcgcgaccat cgccactgaa gaggtgtttt cagttctgtt
    17221 cgacaaccca ttcctgcata ccaccacctt tggcggcaac ccgctggcct gtgcggcggc
    17281 gctggcgacc atcaatgtgt tgctggagca gaacttaccg gctcaggctg agcaaaaagg
    17341 cgatatgttg ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc tggtacagga
    17401 agcgcgtggt aaagggatgt tgatggcgat tgagtttgtt gataacgaaa tcggctataa
    17461 ctttgccagc gagatgttcc gccagcgcgt actggtggcc ggaacgctca ataacgccaa
    17521 aacgatccgc attgaaccgc cactgacact gaccattgaa cagtgtgaac tggtgatcaa
    17581 agcggcgcgt aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt aatatcaaat
    17641 cggatggcga tacgacgtcg ccatccgatc tttttatacg acgcgtacgc ccgcaggcat
    17701 catccgctcc ggcgttaaca gcacgctttc actgccgtca tcggtttccg cacacaacag
    17761 catgcattcc gacgtttcac cacgcatttt cgctttttgc agattgcata agaccaccac
    17821 cgttttcccc atcagctctt cttcgctgta gtacggcacc aggctggtca cggtttgcag
    17881 cgttttttgc cccacatcaa cctgtacgat gtacagcttg tcggcgtttt catggcgttt
    17941 cacttccaca atctttccga cgcgcatttc cagacgtgca aaatcagcgt aagccacggt
    18001 ttccattgct tcctccctta gggtaaaatt ttactaaact atagcaaaag tttttctcaa
    18061 tcctgtaggc taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg ctgtgttacc
    18121 gttacagcgt caaagaaacg cgctttattt actgaaaaca ggtgacccga taagcacttc
    18181 ctctacaatg ggggcgcaca tcagggaaag taaaaaaggt aaacatggca acactaaaag
    18241 acatcgcaat cgaagctggc gtatccctgg cgacagtatc cagggtctt
//
LOCUS       DACAPB010000086        17714 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.086, whole genome shotgun sequence.
ACCESSION   DACAPB010000086 DACAPB010000000
VERSION     DACAPB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.086"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            129..812
                     /locus_tag="IT904_002598"
     CDS             129..812
                     /locus_tag="IT904_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP3192364.1"
                     /translation="MCETYHDEWRIVPAFSSIKKFEEWLEVNDDDPHENGISIEDQDF
                     AANLFRVARKCLSTGRLDDALPLLQRATEQLPEVSEYWLALAIQYRRCKKTEAAAQAA
                     LNAYLGNWAFGVPDNKVIHLLSQAADVPNFQDDPVIQCIKEQGLDLSFGGTKENNNYP
                     LMQMCVDTYFAQRKPLQALTLLHNYAWIMSSETTAFQERYDFNIDEWRAKFRQLCLEY
                     FGDSRTQFT"
     gene            complement(896..2353)
                     /gene="nuoN"
                     /locus_tag="IT904_002599"
     CDS             complement(896..2353)
                     /gene="nuoN"
                     /locus_tag="IT904_002599"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAP3192365.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2360..3889)
                     /gene="nuoM"
                     /locus_tag="IT904_002600"
     CDS             complement(2360..3889)
                     /gene="nuoM"
                     /locus_tag="IT904_002600"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAP3192366.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4053..5894)
                     /gene="nuoL"
                     /locus_tag="IT904_002601"
     CDS             complement(4053..5894)
                     /gene="nuoL"
                     /locus_tag="IT904_002601"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAP3192367.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLIMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5891..6193)
                     /gene="nuoK"
                     /locus_tag="IT904_002602"
     CDS             complement(5891..6193)
                     /gene="nuoK"
                     /locus_tag="IT904_002602"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAP3192368.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6190..6744)
                     /gene="nuoJ"
                     /locus_tag="IT904_002603"
     CDS             complement(6190..6744)
                     /gene="nuoJ"
                     /locus_tag="IT904_002603"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAP3192369.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6756..7298)
                     /gene="nuoI"
                     /locus_tag="IT904_002604"
     CDS             complement(6756..7298)
                     /gene="nuoI"
                     /locus_tag="IT904_002604"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAP3192370.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7313..8290)
                     /gene="nuoH"
                     /locus_tag="IT904_002605"
     CDS             complement(7313..8290)
                     /gene="nuoH"
                     /locus_tag="IT904_002605"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAP3192371.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8287..11013)
                     /gene="nuoG"
                     /locus_tag="IT904_002606"
     CDS             complement(8287..11013)
                     /gene="nuoG"
                     /locus_tag="IT904_002606"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAP3192372.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASAIRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11066..12403)
                     /gene="nuoF"
                     /locus_tag="IT904_002607"
     CDS             complement(11066..12403)
                     /gene="nuoF"
                     /locus_tag="IT904_002607"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAP3192373.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12400..12900)
                     /gene="nuoE"
                     /locus_tag="IT904_002608"
     CDS             complement(12400..12900)
                     /gene="nuoE"
                     /locus_tag="IT904_002608"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAP3192374.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12903..14705)
                     /gene="nuoC"
                     /locus_tag="IT904_002609"
     CDS             complement(12903..14705)
                     /gene="nuoC"
                     /locus_tag="IT904_002609"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAP3192375.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14799..15461)
                     /locus_tag="IT904_002610"
     CDS             complement(14799..15461)
                     /locus_tag="IT904_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAP3192376.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15477..15920)
                     /gene="nuoA"
                     /locus_tag="IT904_002611"
     CDS             complement(15477..15920)
                     /gene="nuoA"
                     /locus_tag="IT904_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAP3192377.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16551..17489)
                     /gene="lrhA"
                     /locus_tag="IT904_002612"
     CDS             complement(16551..17489)
                     /gene="lrhA"
                     /locus_tag="IT904_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAP3192378.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4421 a         4955 c         4580 g         3758 t
ORIGIN      
        1 gcattgatgg aatttgcccg gggccttttc ctccgaacgg atttaccgta cttacagatg
       61 cagcatatgg taacggagac tgtttcggtt tgtactggcc tattggtcag gagcataaac
      121 tacctatagt gtgcgaaacg taccatgacg aatggcgaat cgtacctgcc ttttccagca
      181 ttaagaagtt cgaagaatgg ctggaagtga atgatgatga tccccatgaa aatggcatca
      241 gcatcgaaga tcaagacttt gcagcaaatc tcttccgtgt tgctcggaaa tgtctttcaa
      301 cgggaaggct tgatgacgca ctgccactgt tacagcgagc aacagaacaa ttgcctgaag
      361 tcagcgaata ttggttagca ctggcaatcc agtatcgccg atgtaaaaaa acagaagctg
      421 cagcacaggc tgcgcttaat gcttatcttg gcaattgggc atttggtgtt cctgacaata
      481 aagtcattca tttgctgtca caggccgcag atgtcccgaa tttccaggat gatcctgtga
      541 ttcagtgcat taaagaacaa ggattagatc ttagctttgg aggaacaaag gaaaataata
      601 attacccctt gatgcagatg tgtgttgata cttattttgc gcagagaaaa ccgcttcagg
      661 cattaaccct gttacataat tatgcctgga taatgtccag tgaaacgact gcatttcagg
      721 aacgctatga ttttaatatc gatgaatggc gggcaaaatt caggcagtta tgtttagagt
      781 attttggcga tagtcgtaca cagttcactt aacgctctgc gtctatctac tatcccttct
      841 ttttcagaaa aaccgccgag catgtcggcg gctttctgac ttacaaagta acagattaca
      901 tcagcggcat tgccaaacgc acaatgctaa tcagcggttg tggccataca cccagcacca
      961 gtaccaacag tgcagagatc agcacaacga taccgcccgc gctgtactgc cagtttgatg
     1021 gtgcatcgcg acccggttgt tccggggcgt gaagatacag gctcaccgcc acgcgcaggt
     1081 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
     1141 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
     1201 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
     1261 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
     1321 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
     1381 cttccatcga catctcgccg gtttgcagcg caatcagcgc taccagcaga tagccgaggt
     1441 gagagataga tgagtaaccg agcagacgtt tgatattggt ctggctcagc gccatcaggt
     1501 taccgaagat gatggaggca aaggcgataa tcgccagcac cacgcgaatc gcttcgctgt
     1561 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     1621 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     1681 ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcagg ccgaaacctg
     1741 ccagcaacag cggctcgttg agcataccgt cgccaaggtt tttacccaac gcgacaaacg
     1801 acaggtcgcc agactgcgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg
     1861 cggcagaaag gatggtgtat ttgatactgg cttccagtga acgtttctgg cggaaagcgt
     1921 aaccgaccag gccaaacagc ggcaaagaga tcagttcgat accgaggaac agagacgcca
     1981 gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     2041 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     2101 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     2161 gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc gccgcgttaa
     2221 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     2281 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     2341 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     2401 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     2461 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     2521 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgt aacatcgcca
     2581 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accggtacaa
     2641 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     2701 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     2761 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa
     2821 taaacagacc cgccgccgac aaaccgtgcg caatcatctg gattaccgcg ccctggtagg
     2881 ccaactggct gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt
     2941 aggcgatcag acgtttgata tcggtctggg cgaaggccat ccacgcaccg tagaagatgc
     3001 cgataacacc cagccacata gcaattggcg cgaactctgc cgacgcgttc gggaacagcg
     3061 gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa
     3121 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
     3181 ccggcatttt gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac
     3241 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     3301 agtgaacaaa aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt
     3361 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     3421 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     3481 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     3541 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga
     3601 gcagaccggt cagcacgacc atcagcagcg acagcccgtc aatggcgaga tgaatagaga
     3661 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
     3721 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
     3781 ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc
     3841 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
     3901 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
     3961 cttcggactg ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt
     4021 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
     4081 accacgaccg caccgatgct catggatgcc acataccagc gcagatagcc gttctcactt
     4141 aacagcagac ctttacctgc aaagcgggaa aggacagccg ggatgttcat cattgagttc
     4201 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtca
     4261 tacagccagt caaatcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca
     4321 ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga
     4381 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
     4441 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
     4501 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
     4561 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
     4621 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     4681 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggtag tgccgacagt
     4741 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     4801 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     4861 agcagcgctt taaagaacgc gtgggtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     4921 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     4981 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     5041 atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc
     5101 aggtagacac ccgcggttac catggttgcg gcgtggatca gcgcggagac aggcgtcggg
     5161 cccgccatcg cgtcggcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     5221 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     5281 ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaat
     5341 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgccgct
     5401 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     5461 ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg
     5521 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     5581 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catgatcagc
     5641 gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
     5701 cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     5761 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     5821 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     5881 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     5941 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     6001 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     6061 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     6121 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     6181 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     6241 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     6301 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     6361 tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga ggatggcgta
     6421 aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca cctgcggttt
     6481 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcactacgaa
     6541 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     6601 agcgcccagt gagaagaaca ccccagaaat cgccagcagc gaaataatca ggtacagcag
     6661 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     6721 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     6781 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     6841 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttc
     6901 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     6961 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgcga gaagttgata
     7021 cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     7081 ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc gccgtccggg
     7141 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     7201 tacattcgcg tttcgcgttt tgcgaacgcg tgcaggccga tcatccagat actacgaacc
     7261 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     7321 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     7381 ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca
     7441 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     7501 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
     7561 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
     7621 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca
     7681 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gataacgttc cacacatgcg
     7741 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     7801 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     7861 gcaacgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     7921 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
     7981 tgactggcac aatcgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
     8041 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     8101 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct
     8161 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag gtgacaacca
     8221 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     8281 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     8341 aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagccctt cagcgatttc
     8401 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgtgtgtac ctgcgttcac
     8461 acctaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     8521 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat
     8581 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     8641 attttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     8701 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     8761 cacttgcgaa cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     8821 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     8881 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     8941 cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac
     9001 atggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa
     9061 ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg
     9121 gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct
     9181 ggcagcagaa agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac
     9241 catcaccagc ggtgctttag ccagcgcagc attcacgcgg atagcagaag cgtgacgatg
     9301 cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt cggttaacgc
     9361 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
     9421 catggtgata ccgacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgccgc
     9481 ctgaatcacc tctaagctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
     9541 tgccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac
     9601 cgctggtgca gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
     9661 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt
     9721 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
     9781 agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc
     9841 cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
     9901 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
     9961 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
    10021 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
    10081 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
    10141 aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca ggttgacgta
    10201 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
    10261 gttttcgata cgacgcagtt cgccataacg ctcgccgggg ctgatgttac agccgatgga
    10321 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    10381 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    10441 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg cgccataaac
    10501 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acgcagcggt agcaggcgat
    10561 gcagcggttc atttcgtgag agatgaacgg ccccaaatcc tgattacggt gggtacgttt
    10621 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
    10681 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
    10741 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    10801 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    10861 cttcaccgca cactggcggc aagcaccgac acttcccagc gccggatgcc agcaaaagta
    10921 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    10981 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcagtcag
    11041 agactgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    11101 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    11161 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    11221 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctgacctt
    11281 caccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    11341 tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca
    11401 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctt cccgctttac
    11461 cgatactttc gaattccatc ggcaggtcaa ggtgcgcttc ggtcaggaag tcggtgcccg
    11521 cgccgcctgg ctgccaggct ttgaatttca gaccgtcacg cataccaccg gcgtaatctt
    11581 cgaggatctc gcgcgcggta gtaccaaacg gcagttccca cagtcccgga tttttcaccc
    11641 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    11701 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
    11761 aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag
    11821 cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagata tagcgccctg
    11881 ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca
    11941 agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt
    12001 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
    12061 cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag gtgcccggct
    12121 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    12181 ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt
    12241 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gccccttcgt
    12301 aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    12361 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    12421 ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca tcatgtttgg
    12481 ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt
    12541 cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac cctgataccc
    12601 gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggctgacg
    12661 gaagatctga ctatagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac
    12721 atcggcgatc gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt
    12781 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    12841 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    12901 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    12961 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    13021 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
    13081 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    13141 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    13201 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    13261 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    13321 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    13381 cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
    13441 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    13501 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    13561 tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
    13621 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    13681 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacat cctgaataaa ggtcgagatg
    13741 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    13801 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    13861 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    13921 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    13981 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    14041 ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct
    14101 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    14161 ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    14221 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    14281 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    14341 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    14401 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    14461 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    14521 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    14581 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat caccggatca
    14641 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    14701 accattgcaa atggtccgtg gtaactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    14761 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    14821 aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    14881 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    14941 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    15001 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    15061 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    15121 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    15181 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    15241 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    15301 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    15361 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    15421 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    15481 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    15541 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    15601 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    15661 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    15721 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    15781 ttttttgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcagacaa
    15841 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    15901 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    15961 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg
    16021 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    16081 caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa
    16141 tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc
    16201 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    16261 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac
    16321 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    16381 tatttgtgcg ttcgggacgt gaatctctgg cgattgaaaa atgaacagtt ttgtgcgttc
    16441 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    16501 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata
    16561 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    16621 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    16681 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    16741 agcacacgca ggtccgggct catcatttca accggccttg ccgtcacgcc aagaccggct
    16801 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    16861 tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat
    16921 acaagaggga tcggctcacc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt
    16981 ggcgaggtac gcaggttcaa tgccttaaag gtagaaggac gatgggtggt taccatcaaa
    17041 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    17101 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    17161 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    17221 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    17281 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    17341 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    17401 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    17461 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    17521 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    17581 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    17641 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    17701 ctattaagtt actc
//
LOCUS       DACAPB010000087        17540 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.087, whole genome shotgun sequence.
ACCESSION   DACAPB010000087 DACAPB010000000
VERSION     DACAPB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.087"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..213
                     /locus_tag="IT904_002613"
     CDS             <1..213
                     /locus_tag="IT904_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP3192379.1"
                     /translation="AGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPAA
                     PQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(455..1588)
                     /gene="thiH"
                     /locus_tag="IT904_002614"
     CDS             complement(455..1588)
                     /gene="thiH"
                     /locus_tag="IT904_002614"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAP3192380.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDSYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(1585..2355)
                     /gene="thiG"
                     /locus_tag="IT904_002615"
     CDS             complement(1585..2355)
                     /gene="thiG"
                     /locus_tag="IT904_002615"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAP3192381.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(2357..2557)
                     /gene="thiS"
                     /locus_tag="IT904_002616"
     CDS             complement(2357..2557)
                     /gene="thiS"
                     /locus_tag="IT904_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAP3192382.1"
                     /translation="MQILFNDQAMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(2541..3296)
                     /gene="thiF"
                     /locus_tag="IT904_002617"
     CDS             complement(2541..3296)
                     /gene="thiF"
                     /locus_tag="IT904_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAP3192383.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTSAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(3289..3924)
                     /gene="thiE"
                     /locus_tag="IT904_002618"
     CDS             complement(3289..3924)
                     /gene="thiE"
                     /locus_tag="IT904_002618"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAP3192384.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(3924..5819)
                     /gene="thiC"
                     /locus_tag="IT904_002619"
     CDS             complement(3924..5819)
                     /gene="thiC"
                     /locus_tag="IT904_002619"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAP3192385.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(5900..6000)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6052..6528)
                     /gene="rsd"
                     /locus_tag="IT904_002620"
     CDS             complement(6052..6528)
                     /gene="rsd"
                     /locus_tag="IT904_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAP3192386.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            6623..7396
                     /gene="nudC"
                     /locus_tag="IT904_002621"
     CDS             6623..7396
                     /gene="nudC"
                     /locus_tag="IT904_002621"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAP3192387.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            7436..8500
                     /gene="hemE"
                     /locus_tag="IT904_002622"
     CDS             7436..8500
                     /gene="hemE"
                     /locus_tag="IT904_002622"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAP3192388.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGYGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            8510..9181
                     /gene="nfi"
                     /locus_tag="IT904_002623"
     CDS             8510..9181
                     /gene="nfi"
                     /locus_tag="IT904_002623"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAP3192389.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            9224..9814
                     /locus_tag="IT904_002624"
     CDS             9224..9814
                     /locus_tag="IT904_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAP3192390.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            10001..10273
                     /gene="hupA"
                     /locus_tag="IT904_002625"
     CDS             10001..10273
                     /gene="hupA"
                     /locus_tag="IT904_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAP3192391.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            10286..10981
                     /locus_tag="IT904_002626"
     CDS             10286..10981
                     /locus_tag="IT904_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAP3192392.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(10983..11408)
                     /gene="zraP"
                     /locus_tag="IT904_002627"
     CDS             complement(10983..11408)
                     /gene="zraP"
                     /locus_tag="IT904_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAP3192393.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            11646..13022
                     /gene="zraS"
                     /locus_tag="IT904_002628"
     CDS             11646..13022
                     /gene="zraS"
                     /locus_tag="IT904_002628"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAP3192394.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSRVIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            13019..14344
                     /gene="zraR"
                     /locus_tag="IT904_002629"
     CDS             13019..14344
                     /gene="zraR"
                     /locus_tag="IT904_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAP3192395.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(14341..15630)
                     /gene="purD"
                     /locus_tag="IT904_002630"
     CDS             complement(14341..15630)
                     /gene="purD"
                     /locus_tag="IT904_002630"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP3192396.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDNCFCRKDIGWRAIEREQN"
     gene            complement(15642..17231)
                     /gene="purH"
                     /locus_tag="IT904_002631"
     CDS             complement(15642..17231)
                     /gene="purH"
                     /locus_tag="IT904_002631"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP3192397.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         3915 a         4729 c         4869 g         4027 t
ORIGIN      
        1 gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc
       61 ccggccggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa
      121 gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg
      181 aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
      241 ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca cggaaaatat
      301 taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat tcctgctgac
      361 ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat cttccgtagc
      421 ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag gcgcgtccca
      481 gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac gcggcagcaa
      541 ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg tgattatcgg
      601 catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc agcggaataa
      661 cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc tccggtgcaa
      721 gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc gacgcaggct
      781 caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt
      841 aatgctgttg cagccatagc aaatgttcgg caaccatata gctgtcaacg cgccagttgt
      901 cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc cccagacgat
      961 ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcagatga tggcgggcat
     1021 aagtcgcctc gtgatatgtc tcctgataaa ccatcacgcc atccagacca agttgcttta
     1081 actcggcgta ttccgtctcc gccagcggtt gcacttccat ctgtagtgaa gagaactgtt
     1141 cacgaagggc agggagatga cgacgaaagt aatccatccc cactttcgcc tgatgttcac
     1201 cagtgactaa cagcagatgt tcaaagccca tctcccgtat agcggcactt tccctggcaa
     1261 tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat ccacagtacg
     1321 tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta actgtgttgc
     1381 caaatcgctg acgggtcaga cgctgcgccc gttgggccag ttgttccaga tagccactgg
     1441 cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc
     1501 gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt
     1561 gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccgg tcagcgggct
     1621 ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca gtaggcctgc
     1681 ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga cggcaatcgc
     1741 cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat ggctgggaac
     1801 gccgatgcca gcatcgacaa ccaccggcac tgtggcctgc tggataataa tctccagcat
     1861 ggcgcgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc
     1921 acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt aaggcagcac
     1981 gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa
     2041 cagccagcgg gcgtcagggt gaatctctaa ttttaaccag tttgtgccta acgcttcacg
     2101 agccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggatg tatttggcag
     2161 cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc
     2221 gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg cctccaccat
     2281 cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc
     2341 cgcaatacgt aacatttcaa ccccctgcaa taacctgaaa aagcaggatc tggtcgccat
     2401 cctgcacgat atgttgcgcc cactgctcac gcgggacgat ttgctgatta atcgccagag
     2461 ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg
     2521 cgcactgcat cgcttgatcg ttaaacagga tctgcattgc ttcctccgca taccgggcaa
     2581 ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc gaacagtcgg
     2641 agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag tgcctgcaaa
     2701 gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt gcgttctggc
     2761 tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg cgtcagtacc
     2821 atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg
     2881 caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag caccacatcg
     2941 gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa tgctgtcagt
     3001 tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt cgggcgatcg
     3061 atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca
     3121 tctgccagta ccagcgtccc gacgccagcg cccgccaggt acagcgcagc agatgtaccc
     3181 agcccgccca gaccgataat cagcacctgg ctgtcgagca gtttttgctg cccgtccaga
     3241 gcgatatcgt cgagcaggat ttggcggcta taacgcataa agtcacggtc attcatcgcc
     3301 aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat
     3361 ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg cgcgtgccag
     3421 actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat gccgtgccag
     3481 ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc
     3541 cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt
     3601 cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc
     3661 ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc agtaatcgtt
     3721 gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga catcggcttc
     3781 cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa
     3841 cagacgttcg atccactgta cgctgtccac caccgggtac agtcctgaac gaaaaggtac
     3901 aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat ttctccgcct
     3961 ctggcacgga agttctccga catatccgcc attcccattt caatagtttg cgtggcggcg
     4021 taatcacgca cttcctggct gattttcatc gagcagaatt tcggcccaca catggagcaa
     4081 aaatgggcga ctttacctga ctcttgcggc agggtttcat cgtgataagc gcgggcggta
     4141 aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg ggctttcgac
     4201 atggcgttat cgcgaatttg cgcgcccgga tgccctttcg ccaggtcagc ggcgtgggcg
     4261 gcaatcttat aggtgataag cccctgctta acatcttctt tattgggcag acccagatgc
     4321 tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat cgccgcacca
     4381 atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg ccccagagtg
     4441 taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg gatcatctgc
     4501 atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca ggcaattttg
     4561 gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc gtcctgaata
     4621 gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc acagatttca
     4681 caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag gcaccatttc
     4741 gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt catcggcaca
     4801 tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc ggcctgttcc
     4861 agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt aaccttctcc
     4921 agcgcttggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg
     4981 cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc tccccagcgg
     5041 gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc cgagttgccg
     5101 atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc cgattccgga
     5161 tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc
     5221 gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa
     5281 acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa ttccatttcc
     5341 ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc tgctttggcg
     5401 cgttttggtg ttagtacgcc gctaaaacgc agttcgtcga ggccatcatc tgccagccgc
     5461 gctttagtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc
     5521 cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcaatctg cggatcacca
     5581 tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg cggctgttct
     5641 ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac gcgcacgccg
     5701 gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg
     5761 tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata
     5821 gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca agagacgatc
     5881 gccgcagggg cgatgcgata gcagattaac tcttgttccc ttcgcaggta ttagcctgat
     5941 caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg acaagaaatt
     6001 agccccgcaa atggggcatt gaatgtaaat tacgcgttaa cagcgcagaa ctcaagcagg
     6061 atgtttgacg cgggcggcgt caagcaccag cagaatcagc ttatcttcca gcacaaagcg
     6121 cgcttccagc gcctcgccga tgtcagataa aacctgttga aactcaaggt aattatcatg
     6181 atcgatggcg gtttccagac tggaatcgta gtaatccata atctgttgag tattggcttc
     6241 gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg ttgccttcca gcttatgaag
     6301 aatgcgctca taaatactga aatgtccggc agacaagtaa tcgaccaggc tctgacaaaa
     6361 atcatcaagg gctttttcgt ttagcctcat gtacgattct ttgccaggct taatgccaac
     6421 cagattgtag taagccacga gcagatgctt acgtacatgt agccagcgat caaccagttt
     6481 gttacttcct ctgacgcgct ccgtcaggtt atcgagctgg ttaagcatga ttgactccgc
     6541 aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa ccatgttaaa catgccagtg
     6601 atgcaaaggt agtgcaagag ctatggatcg tataattgaa aaattagatc acggctggtg
     6661 ggtcgtcagc catgaacaaa aattatggtt gccgaaggga gaattgccat atggcgaagc
     6721 ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc ggcgaatggc agggggaacc
     6781 tgtttggtta gtacaacagc agcggcgtca cgatatgggg tcggtacgtc aggtcattga
     6841 tctcgatgtt gggctgtttc aactggccgg acgaggcgta caactggcgg agttttaccg
     6901 atcgcataaa tactgtggtt actgcgggca tgaaatgtat ccgagcaaaa ccgaatgggc
     6961 gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa atcgccccct gcattattgt
     7021 tgccatccgt cgcgatgatt cgatcctcct cgcccagcat acccgccatc gtaacggtgt
     7081 ccatacagta cttgccggat tcgtcgaagt gggcgaaacc ctcgagcagg cagtcgcgcg
     7141 ggaagtgatg gaagagagcg gaattaaagt taaaaacttg cgttacgtga cttctcagcc
     7201 gtggccgttt cctcagtctt taatgaccgc gtttatggcg gaatatgaca gcggcgacat
     7261 cgtgatcgac ccgaaagaat tgctcgaggc gaactggtat cgctatgacg atttgccgtt
     7321 actcccgccg cccggcaccg tagcgcgccg tctgatagaa gatacggtgg cgatgtgtcg
     7381 ggcagagtat gagtgatgat acactgaccg cctgacgcac taaggaacag ccaaaatgac
     7441 cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc cagcccgttg atgtcactcc
     7501 agtatggatg atgcgccagg cgggtcgcta tctaccggaa tataaagcca cgcgcgccca
     7561 ggcaggcgat tttatgtcgc tgtgcaaaaa cgccgagctg gcgtgcgaag tgactttgca
     7621 accgctgcgt cgctacccgc tggatgcggc gatcctcttt tccgatatcc tcaccgtgcc
     7681 ggacgcgatg gggttagggc tctattttga agccggagaa ggtccgcgtt ttacctcgcc
     7741 agtcacctgc aaagctgacg tcgataaact gccaattccg gacccggaag atgaactggg
     7801 ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg aaaggcgaag tgccgctgat
     7861 tggtttttcc ggcagcccgt ggacgctggc gacctacatg gtggaaggcg gcagcagcaa
     7921 agccttcacc gtgatcaaaa aaatgatgta tgccgatccg caggcgctgc acgctctgct
     7981 cgataaactg gcgaaaagcg tcactttgta tctgaatgcg cagattaaag ccggtgctca
     8041 ggcagtgatg attttcgaca cctggggcgg cgtgcttacc gggcgcgatt atcaacagtt
     8101 ctcgctctat tacatgcata aaattgttga tggtttactg cgtgaaaacg acggtcgccg
     8161 cgtaccggtc acgctgttta ccaaaggcgg cggacagtgg ctggaagcca tggcagaaac
     8221 cggttgcgat gcgctgggcc tcgactggac aacggacatc gccgatgcgc gccgccgtgt
     8281 gggcaataaa gtcgcgttgc agggtaatat ggatccgtcg atgctgtacg cgccgcctgc
     8341 ccgcattgaa gaagaagtag cgactatact tgcaggtttc ggttacggcg aaggtcatgt
     8401 ctttaacctt ggtcacggca ttcatcagga tgtgccgcca gaacatgctg gcgtgttcgt
     8461 ggaggcagtg catcgactgt ctgaacagta tcaccgctaa ggagtgatta tggatctcgc
     8521 gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg atccgcgagg atcgactcga
     8581 taaagatcca ccggatctga tcgccggagc cgatgtcggg tttgagcagg gcggagaagt
     8641 gacgcgagcg gcgatggtgc tgctgaaata tccctcgctt gagctggtcg agtataaagt
     8701 tgcccgcatc gccaccacca tgccttacat tccaggtttt ctttccttcc gcgaatatcc
     8761 tgcgctgctg gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg
     8821 tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc cattttggct tattggtgga
     8881 tgtgccgacc attggcgtgg cgaaaaaacg gctctgcggt aaattcgaac cgctctccag
     8941 cgaaccgggc gcgctggccc cactgatgga taaaggcgag cagctggcct gggtctggcg
     9001 cagcaaagcg cgctgtaacc cgttgtttat cgctaccggc catcgggtca gcgtggacag
     9061 cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt ctgccggagc caacgcgctg
     9121 ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa atcagcccta
     9181 attcaggtaa actgcggata atttccgtat ttgagaactc aacatgttac aaaacccaat
     9241 tcatctgcgt ctggagcgcc tggaaagctg gcagcatgtc actttcatgg cttgcttatg
     9301 cgaacgcatg taccccaatt acgccatgtt ctgccagcaa accggttttg gtgatgggca
     9361 aatttaccgt cgcattctcg atctcatctg ggaaacgctg accgttaaag acgcaaaagt
     9421 aaatttcgac agccaactgg agaaatttga agaagcgatt ccttcagccg acgatttcga
     9481 tctgtacggc gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac tggtccattc
     9541 gcgtttgagt ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct ccatcacgac
     9601 cgttgcgatg ctggaaatga ctcaggctgg tcgcgaaatg agcgatgaag agcttaaaga
     9661 aaacccagct gtagagcaag aatgggacat tcagtgggaa atattccgac ttttagctga
     9721 gtgcgaagaa cgcgatatcg agctgataaa aggccttagg gcagacctgc gtgaggcggg
     9781 tgagagcaat attggtataa tttttcagca ataagaccag aaaacgtgat ttaacgcctg
     9841 atttgtcgta cctggagtct tccctttcgc cccccgtctg gtctacattt ggggggcgaa
     9901 aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat tctggcattt ccgtcgcact
     9961 cgatgcttag caagcgataa acacattgta aggataactt atgaacaaga ctcaactgat
    10021 tgatgtaatt gcagagaaag cagaactgtc caaaacccag gctaaagctg ctctggagtc
    10081 cactctggct gcaattactg agtctctgaa agaaggcgat gctgtacaac tggttggttt
    10141 cggtaccttc aaagtgaacc accgcgctga gcgtactggc cgcaacccgc agaccggtaa
    10201 agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga
    10261 cgcagttaag taagattgcg tggcagtgaa cagttttaac gaaggggtgg tttcacccct
    10321 tttgtctttc tggcgtcgat cattgatgct ggctggcgct ctgcttctca ctgcctgtag
    10381 tcataactct tcacttcctc cctttaccgc cagtggattt gctgaagacc agggcgcggt
    10441 acgcatctgg cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg
    10501 gcgcagtggc gataccacga cgcgagagta tcgctggcag ggcgataacc tcacgctcat
    10561 caatatcaat gtttacagca aaccgccggt gaatattcgc gcgcgttttg acgatcgcgg
    10621 tgatctgagc tttatgcaac gtgaatccga tggggaaaag cagcagcttt ctaacgacca
    10681 aatcgattta taccgttatc gtgctgatca gatccgccag attagcgatg ccttacgtca
    10741 ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg tgaccacctg
    10801 cgaagggcaa accattaaac ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg
    10861 ccagagccgc tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca
    10921 attactgtta gtggcaaact ctgatttctg tcgctggcaa cccaacgaga aaacgttctg
    10981 atttaccagt ggcccatacc catatgaccg ccaccaccgc agccgccgta gcccatgccc
    11041 attccggctc cgcgcggaat acccgcttca gccatcgcga tatctcgttt cacccgtaac
    11101 tcatctaacg actgacgcaa attctccatc tctttggcga ccgcgttaat tttgctgcta
    11161 tccggtgggt tcgcggctaa cagggcattg tattcataac gcttcgtcac cagttgctgt
    11221 tgcagtgcgc tgctttgagc gtaaaagtca ttatggattt tctgccacgc tgtctgttgt
    11281 tcgctggtca aaggcgcggc attttgctgc cacataccgt gtccgccgtg agcaaatgca
    11341 gatgtcgatc ccatcgccat tgctgaaagc gccatcatta ccagggcaat tttcgtgttc
    11401 cgtttcatgg ttaatcctcc agtggttgtc ttacttcggg tattgcatct tccgtgccaa
    11461 cgatgaaacg ctgatatgac gggtaatctg gcatgataaa cgagtaaaaa tgactcgcct
    11521 gctgcgggta gcgagtcatt tttactcatt gaaacttaag cctttgtgtt acagcgcagg
    11581 gtaagcgctg ataaaagatg gcatgatttc tgctgtcaga aagggatgag caggcaaaga
    11641 agaagatgcg ttttatgcaa cgttctaaag actccttagc taaatggtta agcgcgatcc
    11701 tccccgtggt cattgttggg ctggtagggt tgtttgcggt gacggtgatt cgcgattatg
    11761 ggcgcgagac tgccgccgcc agacaaacgc tgctggaaaa aggcagtgta cttattcgtg
    11821 ctcttgaatc cggctcgcgc gtcggcatgg ggatgcgcat gcatcatgcg cagcagcagg
    11881 ccttactgga agaaatggcc gggcagcctg gagtacgttg gtttgcggtc acggatgaac
    11941 aaggaacaat cgtgatgcat agcaactccg gcatggtggg aaaacagctt tattcccctc
    12001 aggaaatgca gcagttacat ccgggagatg aagaagcgtg gcggcggatc gatagcgcag
    12061 acggcgagcc tgttctggaa atttatcgcc agtttcaacc gatgtttgct gctggaatgc
    12121 accggatgcg ccatatgcag cagtatgccg cgacaccaca agcaattttc atcgctttcg
    12181 acgccagtaa tattgtgagt gccgaagatc gtgagcagag aaacaccctg attattctct
    12241 tcgccctggc gacggtcttg ctggcaagcg tattgtcatt cttctggtat cgccgctatc
    12301 tgcgctcgcg ccagcttcta caagatgaaa tgaagcgcaa agagaagctg gtggcgctgg
    12361 ggcatcttgc ggcaggcgtt gcccacgaaa tccgtaaccc actttcctcg attaaaggac
    12421 tggcgaaata ctttgccgag cgcgcgcctg cagggggaga agcgcatcaa ctggcgcagg
    12481 tgatggcgaa agaggccgac cgtttaaacc gcgtggtaag cgagttgctg gaactggtta
    12541 agccaacgca tctggctttg caggcggtgg atctcaacac gctgattaac cactcattac
    12601 agctggtaag ccaggatgca aacagccggg tgatccagtt acgctttacc gccaacgaca
    12661 cattaccgga aattcaggcc gatccggaca ggctgactca ggtcctgttg aatctctatc
    12721 tcaatgctat tcaggcgatt ggtcagcatg gcgtgattag cgtgacggtc agcgaaagcg
    12781 gcgcgggcgt gaaaatcagc gttaccgaca gcggtaaggg aattgcggca gatcagcttg
    12841 aagccatctt cactccgtac ttcaccacta aagccgaagg caccggattg gggctggcgg
    12901 tcgtgcataa tattgttgaa caacacggtg gtacaattca ggtcgcaagc ctggagggaa
    12961 aaggctcaac gttcaccctc tggcttccgg tcaatattac gcgtaaggac ccacaaggat
    13021 gacgcacgat aatatcgata ttctggtggt ggatgatgac attagccact gcactatttt
    13081 gcaggcttta ctgcgcggct ggggctataa cgtcgcgctg gcgaacagcg ggcgacaggc
    13141 gctggagcag gtgcgggaac aggtttttga tcttgtgctt tgcgatgtgc gaatggcgga
    13201 gatggacggc atcgccacgc tgaaagagat caaagcgtta aacccggcaa ttccggtgct
    13261 gattatgact gcgtactcca gcgtcgagac ggcggtagag gcactgaaaa ctggggcgct
    13321 ggattatctc atcaagccgc tggatttcga taacctacag gcgacgctgg aaaaagcgct
    13381 cgcgcatacg cacagtattg atgctgaaac acctgcggtg actgccagcc agttcggtat
    13441 ggtcggtaaa agcccggcga tgcaacacct gctcagtgaa atcgccctcg tcgcgccatc
    13501 ggaagccacg gtactgatcc acggcgattc cggcaccggt aaagagctgg tcgccagggc
    13561 gattcacgcc agtagcgcac gtagcgaaaa accgctggta acgctcaact gtgcggcact
    13621 caacgaatcc ttgctggaat ctgaattgtt cggtcacgaa aaaggggcgt ttactggagc
    13681 cgataaacgc cgggaagggc gctttgttga ggcggacggc ggcacgctgt ttctcgatga
    13741 aattggcgat atctcgccga tgatgcaggt gcgtctgctg cgtgcgattc aggagcgcga
    13801 agttcagcgt gtcggtagca accagattat ctcggttgat gtccggctga ttgcggcgac
    13861 ccatcgcgat cttgccgcag aggtgaatgc cgggcgtttt cgccaggatc tctactatcg
    13921 cctgaatgtg gtggcgattg aagtaccatc gctgcggcaa cggcgggaag atatccctct
    13981 gctggctggc cattttctgc agcgctttgc cgagcgtaat cgcaaggcgg taaaaggttt
    14041 tacgccccag gcgatggatc tgttgattca ttacgactgg ccgggaaata ttcgtgagct
    14101 ggaaaacgcg gtggaacggg cagtggtgct gctgaccggg gaatatattt ctgaacgcga
    14161 gctgccgctg gcgattgcca gtacgccgat cccgctggga caaagtcagg atattcagcc
    14221 gttggtggaa gtggaaaaag aggtgattct ggcggcgctg gagaaaacgg gcggcaacaa
    14281 aaccgaagcc gcccgtcagt tagggatcac gcgcaaaacg ctattggcaa aactgtcgcg
    14341 ttagttctgc tcgcgttcga tggcgcgcca gccgatatct ttccggcaga agcagttgtc
    14401 ccagtgaata tcggtcatta aggcataagc gcgtttctgc gcttctgcca cggtatgacc
    14461 cagcgcggtg acgcacagta cgcgcccgcc gctggtcact acctgctcgt catccgccag
    14521 ttttgtgcct gcatggaaca ctttgccgtc ttccacttct tccagcggca ggccgtggat
    14581 cacatcaccg gtgcggtaat cgcccggata tccacccgca gccatcacca cgccgagaga
    14641 agcgcgttca tcccactcgg atgttttctc gtccagcttc ccttcacagg ctgccaggca
    14701 gagctcaacc agatcggact tcatgcgcag cataatcggc tgggtttccg gatcgccaaa
    14761 gcggcagtta aattcgataa ccttcggatt gccctgtttg tcgatcatca ggcccgcgta
    14821 gagaaaaccg gtgtaggtgt tgccttccgc cgccatgcct ttcacggttg gccagatgat
    14881 acgttccatg gtgcgctgat gaacttcatc ggttactacc ggcgcggggg agtaagcgcc
    14941 catcccgccg gtgtttggtc cggtatcttt atcgcctacg cgtttgtgat cctggctggt
    15001 agccatcggc agcacatgct ctccgtccac catcacgata aagctcgctt cttcgccatc
    15061 gaggaactct tcgataacga tgcgatgacc cgcgtcgcca aaagcgttgc ccgccagcat
    15121 atcgtgaaca gccgcttccg cttcttccag cgtcatcgcc acgataacgc ctttcccggc
    15181 agccagaccg tccgctttaa tgacgattgg cgcgcctttc tcacgcagat acgccagcgc
    15241 aggttctacc tcggtgaagt tctggtattc cgccgtaggg atcttatggc gggccaggaa
    15301 atctttggta aacgcttttg agccttccag ttgggccgca cctgcggttg ggccgaagat
    15361 tttcagcccg gcggcgcgga aggtatcgac cacgcctttc accagcggcg cttccgggcc
    15421 gacgatggtc agatcaatct tttcgttttg tgcgaaatcc aacagcgccg ggatatcggt
    15481 tacgccgata gcgacgttct gtagcgcggg ttccagcgca gtgcctgcat tacccggagc
    15541 aacaaaaaca gtttcaacca gcggcgactg cgccgctttc caggccagcg cgtgctcgcg
    15601 cccgccgtta ccaatcacta atactttcat ctattgctcc attaatggcg gaagtggcgc
    15661 atgtcggtga agagcatcgc aataccgtgc tcgtcggcgg cggcaatcac ttcgtcatca
    15721 cggatagaac cgccaggctg gattacgcag gtcacgcccg cagcggcggc ggcatcaata
    15781 ccgtcgcgga acgggaagaa cgcgtcagaa gccatcgagg aacctttcac ttccaggcct
    15841 tcatcggccg ctttaatacc ggcgattttt gcggagtaca cgcggctcat ctggcccgcg
    15901 ccaatgccga tagtcatatt gtttttggca tagacgatag cgttggattt cacaaacttc
    15961 gccaccttcc agcagaacag cgcatcacgc agttcctgtt cgctcggctg acgtttggtc
    16021 accacgcgca gttcttccgc accgaccatc cccaggtcac gatcctgaac cagaagaccg
    16081 ccgttcacgc gtttgaaatc gaggcccgga acacgctcgc cccactgacc gcaggtcaga
    16141 acgcgtacgt tctgtttggc ggcggtgatt ttcagggctt cttcgctggc ggacggcgca
    16201 ataatcactt cgacaaactg acgagaaatg atggcctgtg cggtttccgc atccagctcg
    16261 cggttaaagg caatgatgcc gccgaatgcg gaggttgggt cggttttgta cgcgcgatcg
    16321 taagcatcaa gaatggaatt gccgatagcc acgccgcaag ggttggcgtg cttcacaatc
    16381 acacatgccg gctcggcgaa ctctttcacg cactccagcg ccgcatcggt atcggcgatg
    16441 ttgttataag agagggcttt accctgaacc tgggttgcgg tagcaacgga ggcttctttc
    16501 acattctctt ctatatagaa ggcagcctgc tggtggctgt tctcgccgta acgcatatcc
    16561 agcttcttaa tgaagttcag gttcagcgtg cgtgggaagc gaccggcggc ttctttgctt
    16621 tcaccgtggt aagccggaac catgctgccg aagtagttgg caatcatgct gtcgtaggcg
    16681 gcagtgtgtt cgaaggcttt gatggcgagg tcgaaacggg ttgcaagcgt cagcgatcct
    16741 tcgttgtcat ccatctcttt aataatggcg tcatagtcgc tgctcttcac cacgattgcg
    16801 acatctttat ggttcttggc ggcggagcgc accatcgttg ggccgccgat atcgatgttc
    16861 tcaaccgcat cttccagcga gcaaccttca cgggccacgg tctgggcgaa cggatacagg
    16921 ttaacaacca ccatatcgat aggctggatc tgatgttctt ccataatggc atcgtcctgg
    16981 ccgcgacggc ccagaatgcc accatgtact ttcggatgca gggtcttcac gcgtccatcc
    17041 atcatctccg ggaaaccggt gtaatcggaa acttcggtta ccggcagacc tttttctgct
    17101 aacagacggg cagtgccccc tgtagacagc agctccacac cgcgtgcgga aagtgcctgg
    17161 gcgaattcga cgataccggc tttgtcagaa acactgagca gagcgcggcg gactggacga
    17221 cgttgttgca tggtaaatcc cctggatttg actattacag agagcgttag ctgaattttt
    17281 cgcgaaaaac tcagctaacg cccctaacgg ggcatcctta tttttcgccc gcattgtaac
    17341 gaaaacgttt gcgcaacgct cgcgaatttt tctctttcaa tggtgatcac aattttgaca
    17401 gtggttaccg tgggaaaaat acagaaatta cattgatgat tgtggataac tctgtgcgta
    17461 aaaaggtata aagcggggtt ttgctgggga atgcagcagt cagttatttt tctgcaattt
    17521 ttctattgcg gcctgcggag
//
LOCUS       DACAPB010000088        17335 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.088, whole genome shotgun sequence.
ACCESSION   DACAPB010000088 DACAPB010000000
VERSION     DACAPB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.088"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(284..769)
                     /gene="sixA"
                     /locus_tag="IT904_002632"
     CDS             complement(284..769)
                     /gene="sixA"
                     /locus_tag="IT904_002632"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAP3192398.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(972..3116)
                     /gene="fadJ"
                     /locus_tag="IT904_002633"
     CDS             complement(972..3116)
                     /gene="fadJ"
                     /locus_tag="IT904_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAP3192399.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGAGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3116..4426)
                     /gene="fadI"
                     /locus_tag="IT904_002634"
     CDS             complement(3116..4426)
                     /gene="fadI"
                     /locus_tag="IT904_002634"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAP3192400.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDFNKARIMRQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4606..4890)
                     /locus_tag="IT904_002635"
     CDS             complement(4606..4890)
                     /locus_tag="IT904_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAP3192401.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5262..6602
                     /gene="fadL"
                     /locus_tag="IT904_002636"
     CDS             5262..6602
                     /gene="fadL"
                     /locus_tag="IT904_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAP3192402.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRITLGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6968..7981
                     /locus_tag="IT904_002637"
     CDS             6968..7981
                     /locus_tag="IT904_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192403.1"
                     /translation="MLPSISINNTSAVYTESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRGNSNPERININNSAVTYIDIGSNDTDHITISIDNQEPIKLPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNDVFKRYGRA
                     IRYDFQVDDAKYKCDHLKEIVSTLVGNKINVGHSQKIYKHFKDLEGKIEERLQNRQAE
                     YQN"
     gene            complement(8208..8963)
                     /gene="mlaA"
                     /locus_tag="IT904_002638"
     CDS             complement(8208..8963)
                     /gene="mlaA"
                     /locus_tag="IT904_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAP3192404.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            9257..10189
                     /locus_tag="IT904_002639"
     CDS             9257..10189
                     /locus_tag="IT904_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP3192405.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLIRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNYKNPA"
     gene            10265..10339
                     /locus_tag="IT904_002640"
     tRNA            10265..10339
                     /locus_tag="IT904_002640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10298..10300,aa:Arg,seq:cct)
     gene            complement(10435..11370)
                     /gene="dsdC"
                     /locus_tag="IT904_002641"
     CDS             complement(10435..11370)
                     /gene="dsdC"
                     /locus_tag="IT904_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HAP3192406.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHALTNTVINLCHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            11588..12925
                     /gene="dsdX"
                     /locus_tag="IT904_002642"
     CDS             11588..12925
                     /gene="dsdX"
                     /locus_tag="IT904_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HAP3192407.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSVD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYILVEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            12943..14271
                     /gene="dsdA"
                     /locus_tag="IT904_002643"
     CDS             12943..14271
                     /gene="dsdA"
                     /locus_tag="IT904_002643"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAP3192408.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAIQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(14379..15917)
                     /gene="emrY"
                     /locus_tag="IT904_002644"
     CDS             complement(14379..15917)
                     /gene="emrY"
                     /locus_tag="IT904_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAP3192409.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIVVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIIMPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15917..17080)
                     /gene="emrK"
                     /locus_tag="IT904_002645"
     CDS             complement(15917..17080)
                     /gene="emrK"
                     /locus_tag="IT904_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAP3192410.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         4654 a         4203 c         3961 g         4517 t
ORIGIN      
        1 ttaatcaagt ttcaattaca tcctgcctga ttattcaact ctattttctc tattatctgt
       61 attaataaat gacaatgagt gtcaaacaga cattattcat ttcaacatgt aactttgggc
      121 aaaatcaaga tacgcgcaga agaataattt ccgaatgaaa gtagaaagtg ttttatttat
      181 agatggggag agtcttcgat agtagtgagg ttgacagata tccgctgcct gatgagcaat
      241 tatgcccaat gaatctacct cattgggcat aatttggaac tcatcagata gcttttgcca
      301 tcttcagatt acacgggctc atctgccagt tgaatgtgcc gtttccgctt tcatcaagcg
      361 ttacgctggc aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg
      421 caaccagata cccgactaac ggtaagtgag agatcaccag taccgaagca acaccttcat
      481 tggtcaacgc ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg
      541 gcagaacttc tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct
      601 cggctcgcag gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc
      661 agttcgccat caggcgagat tcgtcacaac cattagtggt cagaggacga accgaatcac
      721 tggcggcatc gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct
      781 tttgttaacc agattcgccc attttctttc atctgaaacc agagaaagaa aacggtggcc
      841 ggcattgtgc cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt
      901 atagtcaata tgcgtttaca ttttgaccaa cactccgcca ttcagcgcgg attcatatag
      961 ctttgacctt cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcgcg
     1021 cccatctcga ccaaacgctc gcaaggggta aaacgggaac catactgcgt ggccagtcgt
     1081 tgcattattg caaccacttc gcccgcgccg agagaatcga tatagcggaa cggtccaccg
     1141 agaaatggcg gaaaaccaat gccaaatacc gcaccaatat ccccgtcacg cacgctacgg
     1201 ataacctgct catcaacaca acgtactgct tcattcagca tcaacatcac acaccgttca
     1261 gcaacctgcg gtgcggagag tcgcccctgc ccttgtgcgc caatgagcgg gtaaatggca
     1321 ggatcgacct gttttttgct tttacgccct ttctgaccat aaagatagaa accccgacca
     1381 ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta
     1441 aaacgttctc cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct
     1501 acctcatcca aaagttggat tgggcctacc ggaaaaccaa atttcactag cgcagcatca
     1561 atgtgctcta cccgttcacc ttcggtcagc atgcggatag cttcattaat gtaaggcgct
     1621 aagatgcgat tgacgtaaaa cccggctttg tcacgcacga caatgggagt tttgccctgt
     1681 tttttcgcca gttttaccgt agtggcgatg gtttgcgccg atgtacccgc atgaggaata
     1741 atctctacca gcggcatttt ttccaccgga ctgaagaaat gcaggccaat aacttgctca
     1801 ggtcgcgcgg cgtgagcggc gatatcacca atcggtaaag atgacgtgtt cgaagcaaaa
     1861 atggtatgag cggcgcaatt ttgctccact tccgccacca tctgttgttt taattcgaga
     1921 ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt
     1981 ccggagatta atgccagctg tttgtcacgt tcgctggctt tgagatgacg acggcgaact
     2041 ttgccctcca gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata
     2101 tctttaattc tgaccggaag ccccgcttta caagcggtga cataagcaat accggcgccc
     2161 atcaagccac cacctaaaat ccccacgctg tttaatggcg caggcggcgc atcactgccg
     2221 ggatctttct tcacgtccgt actggcaaaa aagatactac gcagagcctg cgattgcggc
     2281 gtcatcgcca gttcgccaaa cgcccgtgct tcagcgtcat agccactgct ggtgccctgc
     2341 gctaatcccg tttcgacaac ctccaggatg cgttctgtcg ccggatagtt accttgagtt
     2401 ttgtgttctg ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc
     2461 agaatacgct cgcgtacagg tagagggcgg gaagatgggc gatccttctt tgccagctca
     2521 acagcggctt ccagcagaat ggagtgcgga acgacgtcat ccaccagccc cagctttaat
     2581 gcctgtttcg cccgaagttg ttttccggtg aggatcatct ctaatgctgt gctgacgcct
     2641 atcagacgcg gtaaacgctg ggtgccgcct gaaccgggta acaatccaag ttgtacttca
     2701 ggcaaaccga gcaccgtttt aggatcgtca gtacaaacgc gaccgtggca cgccagcgcc
     2761 aactccagcc caccacccag gcaagcgcca tgaatagccg cgataaccgg aatgggcaaa
     2821 gcatgaatct ccgccatcaa ctgttggccc tgccgcgcca gagcttccgc ttcttgcgcc
     2881 gttttgcagt tgccgatcat gttgatgtct gctccggcga taaaattgtc cggtttcgcg
     2941 gagataaaca ccacgccgcg caactctttg ttttcacgga gttgcttaat aatggcgcgc
     3001 acctgcgagg caaattccgc cttcagggta ttcattttct catccggtac gtcgatggtg
     3061 atgatagcaa tgttgtccag acgaacatta agagtaaacg ctgatgccat ttccattatt
     3121 ccgcctccag aaccattgcc gcgccaagcc caccggcagc acaggcggta actaaaccaa
     3181 atccaccgcc gcgacggcga agttcatgca atgtctgggt aatcatccgt gcgccggtcg
     3241 ccgcgaaggg atgtccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca
     3301 cttcgccagt ggcatgtgca cgccccagta catcacgagc aaaacgttca ctacccagca
     3361 actgaatatt cgccagtgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagat
     3421 cgctcatcgt caaaccggca cgctccagcg ccagcggtgt tgaccaggct ggaccgagca
     3481 acatgtcctg ccagacatca atcgcagtaa atgcgtagct gcgcagatac cccagcggca
     3541 ccagccctaa ttctttcgcc cgggattcag tcatcaggat caccgctgcc gcgccatcgg
     3601 tcagcggcgt actgtttgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca
     3661 gctttgcgta atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt
     3721 gtttataagg agggataaag gcagtcatca cctcttcttt gagttttccg tctgaccatg
     3781 cctgagcggc acgctgatgc gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc
     3841 cgtaggtttt cgccatttgc tctgcggtgt cacccatccg caagccggta gaatattctg
     3901 ctaccgcagg cggtacgggc attaagtcgc gcaaacgcag gcgagagaag agtttcaggc
     3961 gctgtctcat gattcgagct ttgttgaaat caaccagcac gcgcgccagt tttttactga
     4021 cgccaattgg caatacggaa gaggaatctg ccccaccggc aatccccgct cgaatagttc
     4081 ccgccatcag gctttctgcg acgtttgcaa ctgcctggaa actggtagcg caagcgcggc
     4141 tgacgctgta agcatcggta tgcacattca ttcccgtacc gagaacaatt tcacgcgcaa
     4201 tgttgggggc ttcaggcatt tgtacgacct gaccaaagac cagttgttca atcacttcag
     4261 cggggatctc gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg
     4321 gaatgccatg aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg
     4381 cgatacgatc gccctggcgg gtaaccagcg gtaaaacctg acccataaca ctcccctgta
     4441 aaaaataaat aaagtggtct gacctgatca tagtcttaac cattttttta catttagcca
     4501 agcggagaaa agggaaagtg ggagctatga cacagagaga aaagaagaag agaaaagaaa
     4561 acgcccctgc cagcaactga caggggcgta cgtacccgag aggaattaac gcagacccag
     4621 ctggaagatc agcatttcag cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc
     4681 gtcagactct tcagtgaaag tcggggagat tttgcacggt tcggattcca cgctacgggc
     4741 tttctcagtc agcgccgcca gcgtttgttc tgcttctgcg cggtttgcaa acacgcggct
     4801 gtaagacgcg gtgcagtcgg agttgtccat aatggtgcca acatccatac agcagcaaac
     4861 cggggtttca tcagcactac atttactcat cgttgatttc ctctgtatgt gcacccaagg
     4921 tgccagataa acgttgtgga tattttacgc ttccggaaag tgctgctcca gttgttaatt
     4981 ctgcaaaatc ggataagtga ccgaaatcac acttaaaaat gatctaaaac aaaattcccc
     5041 cgaatccatg agtgcgccac ctccaaattt tgccagctgg atcgcgtttc ttagatcata
     5101 tttgaaaaaa gatagaaaca tacttgcaac attccagctg gtccgaccta tactctcgcc
     5161 actggtctga tttctaagat gtacctcaga ccctacactt cgcgctcctg ttacagcacg
     5221 taacatagtt tgtataaaaa taaatcattg aggttatggt catgagccag aaaaccctgt
     5281 ttacaaagtc tgctctcgca gtcgcagtgg cacttatctc cacccaggcc tggtcggcag
     5341 gctttcagtt aaacgaattt tcttcctctg gcctgggccg ggcttattca ggggaaggtg
     5401 caattgccga tgatgcaggt aacgttagcc gtaaccccgc attgattacc atgtttgacc
     5461 gcccgacatt ttctgcgggt gcggtttata ttgacccgga tgtaaatatc agcggaacgt
     5521 ctccatctgg tcgtagcctg aaagccgata acatcgcgcc tacggcatgg gttccgaaca
     5581 tgcactttgt tgcaccaatt aacgaccaat ttggttgggg cgcttctatt acctctaact
     5641 atggcctggc aacagagttt aacgatactt atgcaggcgg ctctgtcggg ggtacaaccg
     5701 accttgaaac catgaacctg aacttaagcg gtgcgtatcg cttaaataat gcatggagct
     5761 ttggtcttgg tttcaacgcc gtctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc
     5821 tggggcagtt ggttgctggt cagattatgc aatctcctgc cgggcaaact cctcaagggc
     5881 aagcattggc agctaccgcc aacggtatcg acagtaatac caaaatcgct catctgaacg
     5941 gtaaccagtg gggctttggc tggaacgccg gaatcctgta tgaactggat aaaaataacc
     6001 gctatgcact gacctaccgt tctgaagtga aaattgactt caaaggtaac tacagcagcg
     6061 atcttaatcg tgcgtttaat aactacggtt tgccaattcc taccgcgaca ggtggcgcaa
     6121 cgcaatcggg ttatctgacg ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc
     6181 gtgttgatcc acagtgggcg attcactata gcctggctta caccagctgg agtcagttcc
     6241 agcagctgaa agcgacctca accagtggcg acacgctgtt ccagaaacat gaaggcttta
     6301 aagatgctta ccgcatcacg ttgggtacca cttattacta cgatgataac tggaccttcc
     6361 gtaccggtat cgcctttgat gacagcccag ttccggctca gaatcgttct atctccattc
     6421 cggaccagga ccgtttctgg ctgagtgcag gtacgactta cgcatttaat aaagatgctt
     6481 cagtcgacgt tggtgtttct tatatgcacg gtcagagcgt gaaaattaac gaaggcccat
     6541 accagttcga gtctgaaggt aaagcctggc tgttcggtac taactttaac tacgcgttct
     6601 gataacacgt tcgcctggat aaagtcacct gcatagcagg tgactttaac tcccccactt
     6661 caccgaaagt agtgtcctca ttgcttacct cctgagtttt gcaaacagcc tgacgtcagc
     6721 ttaccctttc aaatcaatca gtggtatcca cagacaaacc tgaaggggaa cgcatttttt
     6781 caggcgttct gctcgtcctt ctcaaagagt ttacttttct gaatttccag gatactcacc
     6841 ccccctggat attgtgcgct catacacgca aattaaagat aggttctaaa taaatgagcg
     6901 ttttttgata gtctatttta ttaggtaata tatatttgta acaaatcaat caacatggaa
     6961 taaaatcatg ctaccatcta tttcaatcaa caataccagc gctgtttaca cagaatccat
     7021 caatgaaaat aacaatgatg aagttaatgg attagtacaa gagttcaaaa acctttttaa
     7081 tggtaaggaa ggaataagca cctgtattaa acatctactt gagcttataa aaaatgccat
     7141 acgaggaaac agcaatcctg agagaattaa tatcaataat tccgcagtta cttatatcga
     7201 tattggctcc aatgatacag accatattac tattagtatc gataaccaag aaccaataaa
     7261 attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt
     7321 tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata
     7381 tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac atacggtaac
     7441 ggaatttgat gagagcggga aaagcgaacc aacggactta tttacctggt acggtaaaga
     7501 taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc
     7561 tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga
     7621 tagtctcacc caatattgta gtgaaaacgc caggagtgct tcagcgtggt ttgaaagcag
     7681 taaagctatc atggcagaat catttgcaac tggttccgat catcaggttg taaacgagct
     7741 caacggggaa agactgagag aaccaaacga cgtttttaaa cgttatggtc gagcaataag
     7801 atatgatttt caagtggacg atgcaaaata taaatgcgac catctaaaag aaatagtttc
     7861 tactttagtc ggtaacaaaa ttaacgttgg ccattctcaa aaaatatata agcattttaa
     7921 ggatctcgaa ggtaaaattg aagaaaggct tcaaaatcgc caggctgaat atcaaaatta
     7981 aattaatcaa ccatctgcgc caggtgttaa ttttgatgat atttaaaatt atttttccag
     8041 ttttttgatt tttcaacagc tgaatatcgg aaatagtcag tgacgaacat cagtattttt
     8101 acataattac gtctaggtca ttgtcggttt atctcctttt acttgtggta attgagggat
     8161 aacaacaaaa aaggtgagtt ttgcaactca ccttttttat ttgtttctta ttcagaatca
     8221 atatctttta aatcatcctg aatcgcttgt gcgttcggat tttcctgcgg tttgagttcg
     8281 ccgccattag cgatgaaatc atgacgctgg aagtacgctt cgcgcaccat aatataagga
     8341 tcggacgact gacgcagcag accatcggaa tccagcagct gagcgcgggt ttcgatccct
     8401 tcaagcgtcc atttacccac agacatcggc caggtcagcc aggaaagaac cgggtaaaga
     8461 ccatccgcca tatcaccacc gtcatcacgc agcgtgaagc taccgtagaa cggtaactga
     8521 acgtaaggcc cataacccac gccataatga ccaagcgtac taccgaagcg gtgaggttca
     8581 gtccgttgca gtttcgggtt cgccatccct gcaacatcaa taaagccgcc catccccaaa
     8641 atggtgttca ggaaaaagcg ggtaaagtgg accatcccct gataagggtc gccctgcaag
     8701 aagtagttaa ccatcaccgc aggttcttca aggttgccag taaagttgct caaaccatta
     8761 cgcgccggtt gcggaacata atcacgccag gcgacagcga ccggtcgaac aatatacggg
     8821 tctaatacgt tgaagttgaa gttgtacatg gtgcggttga acccttctaa cgggtcagaa
     8881 cgcccttgct gatctgtacc ggaactcgca caccccacca gaagcgtagt tcccagagca
     8941 agcgccgaca ggcgaagctt cataaatgtc tccctgtttt tttatggctt atgcagtttg
     9001 ccatccatga cggaacgata ccgtatccgc ctgtttaggt gtgggcgatt gtaacagcac
     9061 gtcaactgat gtccagacgc cctgatttgc tgatttgatc atagcctggt aatcgccgcc
     9121 ctgtaggcta cttgattcta tagaaacaga aaaaggcaaa cgcagccttt tctacatttt
     9181 cagagtaact cccgtcagtt gcgagcaaaa aagccgctac gctttagcta tacgtgctaa
     9241 ttcaagagaa gagaccatgg acaacgacaa aattgatcaa cacagcgacg aaattgaagt
     9301 tgagagcgaa gaaaaagagc gcggcaaaaa aatagaaata gatgaagacc gactcccctc
     9361 ccgggcgatg gcaattcatg agcatatccg ccaggatggt gaaaaagagc tggaacgcga
     9421 cgcaatggcg ctactgtggt cagccattgc ggcgggtctg tcgatgggcg cttcgttact
     9481 ggcaaaaggg atatttcatg tcgaactgga aggtgtgccg ggcagcttct tgctggagaa
     9541 tctcggttat acctttggtt ttattatcgt cattatggcc cgccagcaat tatttaccga
     9601 aaacaccgtg actgcggtac tacccgtcat gcaaaaaccg acaatgagca acgtcggctt
     9661 acttatacgg ttatggggcg tcgtgctgct gggtaatatt ctcgggacag gtattgcggc
     9721 gtgggcattt gaatatatgc ctatcttcaa tgaagaaact cgcgatgcat ttgtcaaaat
     9781 cggcatggat gtgatgaaga acacccccag cgagatgttt gccaacgcga tcatttccgg
     9841 ctggctgatc gccactatgg tttggatgtt tcctgcagcg ggtgcggcaa agattgtggt
     9901 gattatattg atgacctggc ttattgccct gggtgacacc acccatatcg tggtcggttc
     9961 tgttgaaatc ctctatctgg tgtttaacgg tacgctgcac tggagcgatt tcatctggcc
    10021 cttcgcacta cctactttag cggggaacat ctgcggcggc acctttatct tcgcgttaat
    10081 gagtcatgca cagattcgta acgacatgag caataagcgt aaagcagaag cacgccaaaa
    10141 agcagaacgt gcggaaaaca ttaagaaaaa ttataaaaac ccggcataaa tggcgagggt
    10201 ttaagcaatc gagcggcagc gtacttaccc cgcactccat tagcgggtat actcatgccg
    10261 cattgtcctc ttagttaaat ggatataacg agcccctcct aagggctaat tgcaggttcg
    10321 attcctgcag gggacaccat ccttgtgttt atccctaaaa ccacataaaa accgtaaatt
    10381 aaattcgaac tagcaggccg aataaggcac accaggaact cgtggtttta ttcattatga
    10441 agtggttttt acctgttctc ttagccaggt aataaacgct tcaatttttg gccactgcct
    10501 gcccggtaat gtggtgatgt aataatgctg atggcatttc accgtcatat cgccaaacgg
    10561 cgcgacgagc tcaccactgg caagcctttt ttgtaccagg cgttttcttc ccatcgccac
    10621 cccaatatga ttcatcgcgg caataacagc taaatcagaa cgatcaaagc caattccaga
    10681 agatgtcggc aaattaaccg cataatgttg cgcccaacta tgccattcat ccgtaccgga
    10741 gtcgttgctc catgcctgtc tgtcatggag caacgtacag tgacacaggt taattaccgt
    10801 gttggttaaa gcatgtcttt gagcgtattc cgggctacat actggcagga tttcttcatc
    10861 catcagaaag tgatgagtga gttgcgctga cggcgcatca tcaaagtaga tcgccaaatc
    10921 gattccggca cgttgcagat tgacgttgtc attaccagtg agcacggtga gcgaaataga
    10981 cggataccgg cgtgtaaagt cacctaatgc gggcaccaac cagcattggg cgatagaggg
    11041 ccgggaatac agagttaacg ttcccgataa ctcctgattt ttgatatcaa gaatttcctg
    11101 gttcagggta tccagcgacg attttagcgc ccaataaaca cgtttcccct cgtgcgttaa
    11161 ttccactttg cgatgggaac gaacaaacaa ctgaatgccc aattcttctt ccagctgatt
    11221 gatacggtga cttaccgcac tggggctcag cgacaattcc tctgccgcca gggcgaagga
    11281 ctgatgcctg gcagccactt caaaagtatg catttttgat aattgccagc cgttaagcag
    11341 ccgatttctt atttcacgaa ggggttccat aatcacctca tttttcgctt aagtgtaaaa
    11401 aaatagcggc aaaatttcag ctatgagatg agctaaagtg aaccatatct caattcacct
    11461 tcatttttag atgtaaatca ctccattgat gcaatttacc tcatgtgaaa ggcaaatttt
    11521 atcgtttgtc agcctgcgtt gtttttttgt ccaatatcat caggttaatc acaggggaag
    11581 gtgagatatg cactctcaaa tctgggttgt gagcacgctg cttatcagca tcgtgttaat
    11641 tgtactgacc atcgtgaagt tcaaattcca cccgtttctg gcgctgttgc tggccagctt
    11701 cttcgtggga acgatgatgg gcatggggcc actggatatg gtaaatgcta ttgaaagtgg
    11761 aattggcgga acgctggggt tcctcgcagc ggttatcggc cttggcacga tactgggaaa
    11821 aatgatggaa gtatccgggg ccgcagaaag aattggtctg acacttcaac gctgccgctg
    11881 gctttcagtt gatgtcatta tggtgctggt tggcctgatt tgtggcatca cgctgtttgt
    11941 tgaagtgggc gtcgtgctat tgattcctct ggctttttca attgccaaaa aaaccaatac
    12001 ctcattatta aagcttgcca ttccgctatg taccgcattg atggcagtgc actgcgtggt
    12061 tcctccacat ccggctgctt tatatgttgc caataagctg ggcgcagata tcggttcggt
    12121 gatcgtctac ggtttgctgg ttgggctgat ggcatcactg atcggtggcc cacttttcct
    12181 taaatttctg ggtcaacgac tgccctttaa acctgtaccc acagagtttg cagatctcaa
    12241 agttcgcgat gaaaaaacac taccgtcatt aggcgcaacg ttattcacca tactgctacc
    12301 cattgcgctg atgttggtta aaacgattgc cgaattgaat atggcgcgtg agagtggttt
    12361 gtatatcttg gttgagttta ttggcaaccc tatcactgcc atgtttatcg ccgtgtttgt
    12421 cgcctattat gtgttgggta tacgccagca tatgagcatg gggacgatgc tcacacatac
    12481 ggaaaatggc ttcggttcta ttgctaatat tttgctgatt atcggggccg gaggcgcatt
    12541 caacgccatt ttaaaaagca gcagtctcgc tgatacgctg gcagttattc tctccaatat
    12601 gcatatgcac ccgattcttc tggcctggtt agtggctctt attctgcatg cggcagtggg
    12661 ctccgctacc gtggcaatga tgggggcaac ggcaattgtt gcacccatgc tgccgcttta
    12721 tcccgacatc agcccggaaa ttattgcgat tgctatcggt tcaggtgcaa ttggctgcac
    12781 gatcgttacg gactcgcttt tctggctagt gaagcaatat tgcggcgcta cgctcaatga
    12841 aacatttaaa tactatacga cagcgacatt tatcgcttca gtcgtcgctc tggcgggcac
    12901 attcctgctg tcatttatca tctaagcgca aagagacgta ctatggaaaa cgctaaaatg
    12961 aactcgctca tcgcccagta tccgttggta aaggatctgg ttgctcttaa agaaaccacc
    13021 tggtttaatc ctggcacgac ctcattggct gaaggtttac cttatgttgg cctgaccgaa
    13081 caggatgttc aggacgccca tgcgcgctta tcccgttttg caccctatct ggcaaaagca
    13141 tttcctgaaa ctgctgccac tggggggatt attgaatcag aactggttgc cattccagct
    13201 atacaaaaac ggctggaaaa agaatatcag caaccgatca gcgggcaact gttactgaaa
    13261 aaagatagcc atttgcccat ttccggctcc ataaaagcac gcggcgggat ttatgaagtc
    13321 ctggcacacg cagaaaaact ggctctggaa gcggggttgc tgacgcttga tgatgactac
    13381 agcaaactgc tttctccgga gtttaaacag ttctttagcc aatacagcat tgctgtgggc
    13441 tcaaccggaa atctggggtt atcaatcggc attatgagcg cccgcattgg ctttaaggtg
    13501 acagttcata tgtctgctga tgcccgggca tggaaaaaag cgaaactgcg cagccatggc
    13561 gttacggtcg tggaatatga gcaagattat ggtgttgccg tcgaggaagg acgtaaagca
    13621 gcgcagtctg acccgaactg tttctttatt gatgacgaaa attcccgcac gttgttcctt
    13681 gggtattccg tcgctggcca gcgtcttaaa gcgcaatttg cccagcaagg ccgtatcgtc
    13741 gatgctgata accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg tcctggtggc
    13801 gtcgcattcg ggcttaaact ggcgtttggc gatcatgttc actgcttttt tgccgaacca
    13861 acgcactccc cttgtatgtt gttaggcgtc catacaggat tacacgatca gatttctgtt
    13921 caggatattg gtatcgacaa ccttaccgca gcggatggcc ttgcagttgg tcgcgcatca
    13981 ggctttgtcg ggcgggcaat ggagcgtctg ctggatggct tctataccct tagcgatcaa
    14041 accatgtatg acatgcttgg ctggctggct caggaagaag gtattcgtct tgaaccttca
    14101 gcactggcgg gtatggccgg acctcagcgc gtgtgtgcat cagtaagtta ccaacagatg
    14161 cacggtttca gcgcagaaca attgcgtaat gccactcatc tggtgtgggc gacgggaggt
    14221 ggaatggtgc cggaagaaga gatgaatcaa tatctggcaa aaggccgtta ataacgtttc
    14281 aacgcagcat cgcaatcctt tccctgggtg agcgatgctg ccgatggcgc agacttaaga
    14341 tccccggtct tacccgctat aaccccctcc ttttatcatc acccaacgcc tttcgctgta
    14401 aacggcggtt tcgcaaacca aaccaaaacc gtaagtaaga taaaagcaat agccgccata
    14461 cggaaaatct catttgcaga aatagaaagt gactgctggg ttatttcatt attaatttca
    14521 ttaagaactc ctgaaagcga accatagtat ttatccataa tttgtgacga tgaattaaac
    14581 acggggttaa attgatcgat ggttgctgtc aactgactat ggtgtaacga ttcgcgtcgt
    14641 ccccacagcg tcattgtcaa cgacgtacca actgatcctg acaaggtacg aaaaaaatta
    14701 ctcatactcg aggcattggc aaatttatta tctggcaagc ctgaaaacga aatcgttgtt
    14761 aagggtaaaa agaaacaggc aacggcgaat ccctgaaaaa actgcggcat aatgatacct
    14821 gtaaaatcaa tcgttggcat aaatgtcaca gaacgccagt aatagcaaac cgcatacatc
    14881 aaaaaactga atgtcaccaa cacccgcatg tctattttgt tgccataacg tcctatcaaa
    14941 ggtgaaatta atagtggcat gatgccgatg ggcgcataag caagtccggc ccatatcgca
    15001 ttatacccca tcgtttcctg gagtaactgc ggcataagga cgatcgctcc agagtaaaat
    15061 aaatacgcgc atgtgatact cacaatacca atggtgaagt tacgggactt aaacaaactg
    15121 agatcaagaa tcgggttctc tgaggtcgac tcccaaatga ctaaagagat cagagaaatg
    15181 actgatacta ctgttaatat tattatcgta ctcgagttga accaatccag atcgcgccct
    15241 ttatcaagca taatttgcaa gccaccaaca ccgagcacta acagggtcag tcctggtaga
    15301 ttcattttga ccggtgaagt ctcagtttct cttcctttaa gtaaggttaa gcataatgtc
    15361 aggacgacaa tccccatagg gacattgatt aaaaatatcc aaccccagct aaagttatca
    15421 caaatatagc cgcccaatat cggcccacat atcggagcga taatcacggt cattgaccat
    15481 aatgccagag caaatgtcct tttttctggt gggtaattcc ttaataataa actctgtgac
    15541 agtggaatta acggccccgc cattaacccc tgaacgactc taaaaaatat cagcacatca
    15601 agattggtcg ataggctaca cattaatgaa gacagcgaaa aaaaggttac tgaaagtaaa
    15661 aataatctta attcgcctat tctttgtgcc aacctgccag taacagggat cgcaatggca
    15721 tttgctacac caaacgaggt gataacccag gtgccttcgt ctgttgatgc tcccagaaag
    15781 ccagatattg tcggtattgc gacgttagaa atagtggaat ccaacatttg cataaatgtc
    15841 gctaatgaca atgcaatagt gacgcaccat aacgtcccac cggttaatgg tgccggagtt
    15901 gatttagtga ttgccattaa agttgtccat tatgcgaaat aatgttgcta atttcttttt
    15961 ctatcggact ggtatcgata actaaagcct tactggtata agccggcatg gaggtcacgg
    16021 ttgaagccag ctcaggcatc tcggcaatgt cttcgttctt cgtatcaata gttgctgtca
    16081 tcgataaacc aatacgcaag ggatgttcca tgagttcttt tggatcaaga gaaacttcaa
    16141 ccggtacacg ctgaacgatt ttgatccagt tccctgtcgc attttgtgca ggtaataagg
    16201 agaacgcatt gccggttccc atattgatcc ctgtcacccg accatgaaac acaacatttt
    16261 caccataaag atcgctgata atattgaccg attgaccaat ccgtacatcc gtgagttgtg
    16321 tttctttaaa gttggcatta acccacattt gacgtgccgg tacgacagcc attaacgatt
    16381 gtccggggct cactgtttcg ccgacctgaa cacttctctg ggcaatatag ccggtaaccg
    16441 gactcttaat atccgtacgt ttaagcgcca accaggcttc tttagttgca tccgccgctt
    16501 caacgacttg tggctgacgg tttaatggtg tgttcattac taaagcttta ttcgctttat
    16561 aagcctggat agcggcattc aatgccgctt tgctacttat taacgtatct ttggtatgct
    16621 ccagcgtttc ttttgaaata accccctgct tcgctaacgg cactcgacgg ttataatctt
    16681 ctaaagattg ttgatactga atacgtgctg aagcgacttc ggcactgtat tgtttatcct
    16741 gtaagtatag tttattcgtt tgccgaacaa tatttgccag attattttta gctttattga
    16801 gtgcgatagt ggcatcagtt ttatccagtg aaactaaaat gtcaccttgt cgaacgtagt
    16861 tcgtatcttt atgattaacg acagtgacac tacctgagac ttgtgcagaa attggatctg
    16921 cattccccgt gacataggcg tcatctgtac taatcatgtc ttctaattcc attgaccaat
    16981 aggcataggc acctgaaaac gcaataaata aaactaccgc caataaagaa aagtattttc
    17041 ttctgttaga atgtttttta tttgaattaa tctgttccac tattatctct catttctcat
    17101 agatgaaatt tatgaattga atgaaaagag cattcattct tcatatcaaa cacagtgcat
    17161 gtgttgcaca ttatcccagg aacgttggag gcctaattta aatgaggtgc aaaaaaaaca
    17221 acagaggact aaaccgtggc ttttgcaata caattcttac gcctgtagga ttagtaagaa
    17281 gacttatagt gccaacttga aactataaat catcggtaca atccctgatt ttatt
//
LOCUS       DACAPB010000089        17204 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.089, whole genome shotgun sequence.
ACCESSION   DACAPB010000089 DACAPB010000000
VERSION     DACAPB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.089"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1419)
                     /gene="dinG"
                     /locus_tag="IT904_002646"
     CDS             complement(<1..1419)
                     /gene="dinG"
                     /locus_tag="IT904_002646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAP3192411.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTVPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWH
                     SIPHIIVTSATLRSLNSFSRLQE"
     gene            1539..2021
                     /gene="ybiA"
                     /locus_tag="IT904_002647"
     CDS             1539..2021
                     /gene="ybiA"
                     /locus_tag="IT904_002647"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAP3192412.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(2253..3617)
                     /gene="rhlE"
                     /locus_tag="IT904_002648"
     CDS             complement(2253..3617)
                     /gene="rhlE"
                     /locus_tag="IT904_002648"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAP3192413.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            3846..4517
                     /gene="cecR"
                     /locus_tag="IT904_002649"
     CDS             3846..4517
                     /gene="cecR"
                     /locus_tag="IT904_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAP3192414.1"
                     /translation="MNNPVMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            4517..5515
                     /gene="hlyD"
                     /locus_tag="IT904_002650"
     CDS             4517..5515
                     /gene="hlyD"
                     /locus_tag="IT904_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAP3192415.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            5508..7244
                     /locus_tag="IT904_002651"
     CDS             5508..7244
                     /locus_tag="IT904_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3192416.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLRRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVGGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            7237..8370
                     /locus_tag="IT904_002652"
     CDS             7237..8370
                     /locus_tag="IT904_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3192417.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            8381..9487
                     /locus_tag="IT904_002653"
     CDS             8381..9487
                     /locus_tag="IT904_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3192418.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRSTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(9449..9859)
                     /locus_tag="IT904_002654"
     CDS             complement(9449..9859)
                     /locus_tag="IT904_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192419.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            9992..10753
                     /locus_tag="IT904_002655"
     CDS             9992..10753
                     /locus_tag="IT904_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP3192420.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAELDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLQTWRHLSDHAPLSAEIHL"
     gene            10750..11991
                     /gene="clsB"
                     /locus_tag="IT904_002656"
     CDS             10750..11991
                     /gene="clsB"
                     /locus_tag="IT904_002656"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAP3192421.1"
                     /translation="MKCSWREGNKIELLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            11991..12947
                     /locus_tag="IT904_002657"
     CDS             11991..12947
                     /locus_tag="IT904_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAP3192422.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHITKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(12983..13696)
                     /locus_tag="IT904_002658"
     CDS             complement(12983..13696)
                     /locus_tag="IT904_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP3192423.1"
                     /translation="MESYSKNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(13902..14606)
                     /locus_tag="IT904_002659"
     CDS             complement(13902..14606)
                     /locus_tag="IT904_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP3192424.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(14743..15195)
                     /gene="moaE"
                     /locus_tag="IT904_002660"
     CDS             complement(14743..15195)
                     /gene="moaE"
                     /locus_tag="IT904_002660"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAP3192425.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDRQAAKRW"
     gene            complement(15197..15442)
                     /gene="moaD"
                     /locus_tag="IT904_002661"
     CDS             complement(15197..15442)
                     /gene="moaD"
                     /locus_tag="IT904_002661"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAP3192426.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(15435..15920)
                     /gene="moaC"
                     /locus_tag="IT904_002662"
     CDS             complement(15435..15920)
                     /gene="moaC"
                     /locus_tag="IT904_002662"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAP3192427.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGSVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(15923..16435)
                     /gene="moaB"
                     /locus_tag="IT904_002663"
     CDS             complement(15923..16435)
                     /gene="moaB"
                     /locus_tag="IT904_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAP3192428.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(16457..>17204)
                     /locus_tag="IT904_002664"
     CDS             complement(16457..>17204)
                     /locus_tag="IT904_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAP3192429.1"
                     /translation="DIIAAVRENDAIRQIAVTTNGYRLERDVANWRDAGLTGINVSVD
                     SLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVKVNTVLMRDVNHHQLDTFLNWIQH
                     RPIQLRFIELMETGEGSELFRKHHISGQVLRDELLRRGWIHQLRQRSDGPAQVFCHPD
                     YAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLFGEGGVNLRDLLEDDAQQQALEAR
                     ISAALREKKQTHFLHQNNTGITQNLSYIGG"
BASE COUNT         4048 a         4603 c         4539 g         4014 t
ORIGIN      
        1 ctcctgcaaa cgcgaaaaac tgttcagcga acgcaaggtt gcggaggtga caataatgtg
       61 cggaatactg tgccacagca gcctttccag ctgatcgctg acacgtattc ccacgcagtg
      121 aaaccagagg tgtagctgcc cttcgcgctc ttcccgcgtc gcccatttgg tcaccggtgc
      181 accggaagat tgcgccagcg aagccagccg ccagagtttg ctttgcgcct cgaacatccc
      241 caacgcgcgg ttcatctgca aaatcaaccg atgcagacgt acaatgtcat ggctgccggt
      301 tttctcactt aaatcgttaa gaaataactc cgccaggcca cgcagcatct cggtgagttt
      361 tgccagccgc tggcagatct ccagcacttc atctggcagt tcgcccatcg caaaacggtg
      421 ctctgcctcc tgcccggcag gcatgtagag attgagaatg ttgtttaacg aggcgataag
      481 ctcatacaac tcttcacaat gcgcattcaa acgttcaggg atcgccagcg gtgggatggt
      541 cttcgggcga aactgctcca tgcaggtagc gaccagtttc gtgaacaagt ccagctgtag
      601 ccgataccac ggcgcggtga tttcggcgct catctcaagc gcatcccgcg ccacatccgg
      661 caggtgatgg ccttcgtcca gcaccagcag taaatttttc gggtcaggca ataccgcttc
      721 gctttccatc gccgccatca ccagcgcatg gtttgccacc accacttccg cttcctgaat
      781 ctcccgacga gcgacaaaaa acgggcattc acgatagtag taacaattgc ggttgaggca
      841 gctggctttg tcggtactta aacgacgcca gagatcgtca tctatagcga tatcagtatg
      901 atcgcgcaga ccatcccatt tataagtatc gagatcgccc ttcagcttcg cacaacgttt
      961 ttgctcttcc tgattgttcg gcgtcagttc gtcgtcaaga aacgccagca gatcctgttg
     1021 cgtgggtaca gtactggcga gcgccgtcag attacgcgga caaacgtagc gcccacgccc
     1081 aaaagcggca gtgaatttaa gatcgggaat gatctttttc agcagcggta aatctttgct
     1141 gtaaatctga tcctgcaatg ccacgttggc ggtactcacc accagcgttt tttgctcttc
     1201 gcgggcaatg gcgatgccgg gaatcaaata agagagcgtt ttcccaacgc cggtgggggc
     1261 ttcaatcgcc agatgtcgcc cttcttctcc ggccagcgtt ttggcgacgt ccgcaatcat
     1321 ctgccgctgc ggcgcacggg gaataaagtc ggggatctgt tcctgaagcg ccttatacca
     1381 ggcggcaatt tgcgctttaa gcgcggcggt taatgccatg agaaaacctg aaatactgta
     1441 taaacagcca atattgtggc atttttcggt tttcaggggt aatggttttt ctgtttctgg
     1501 aaagcggctc gcaataacca ccaacataat gcggtttaat gcccgttcga gcacaaagga
     1561 tccaacacgt catgcaagac accatcataa atttctacag caccagcgat gactacggtg
     1621 atttttctaa ctttgccgcc tggcccatca aggttgacgg gaaaacctgg cccacctcag
     1681 aacactattt tcaggcacaa aaattccttg atgaaaaata ccgcgaagag atccgtcggg
     1741 tttcttcccc tatggtcgcc gcacgcatgg ggcgcgatcg ttctaagcct ctgcgtaaaa
     1801 actgggagtc ggtcaaagaa caggtgatgc gaaaagcact tcgcgctaaa ttcgaacagc
     1861 atgcagaact gcgtgcgctc ttgctggcaa ccgcgcccgc aaaactggtt gagcatacgg
     1921 aaaacgatgc ttactgggga gacggtggtc atggtaaggg caagaatcga ctgggctacc
     1981 ttttaatgga gttgcgcgaa caattggcta tagagaagta acgtctcggt ggcttatggg
     2041 cggtaaaaca gataaatttt ttcccgtgtt ctcaaattgc tgacaagtgc ttgttgtgaa
     2101 atgccggatg cggcgtaaac gccttatccg gcctacaaaa gcatgcaaat tcaatatatt
     2161 gcagagttca cgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgttcatca
     2221 gcctgatgcc gggcatagcc cggcataaaa gattactgcg cagcggcagg tttacgcgga
     2281 cggcggcgac gttgttgttc gcctgccggt ttggcatcgc cgaggcggcg agacggtttt
     2341 tctgcaggtt tcgcgcttgc agattttgcg ccaccttccc cacggcgtgg ttgctgttga
     2401 ccgcgaccac caccttgccc acgaccgccg ccgccacgtt gctggcgacc gttctggatc
     2461 ggttcggctt tgattgacgg gtccggctca tagcccggaa tcgcaatgcg cgggatctct
     2521 tttttcagca gtttttcgat atcacgcagc agtttgtgtt catcaacaca caccagcgac
     2581 aacgcttcac cggtagcagc cgcacgaccg gtacgcccga tacggtggac ataatcttca
     2641 ggtacgtttg gcagttcata gttgacgacg tgcggcagct cttcaatatc caggccgcgc
     2701 gcagcgatgt cagttgccac cagtacacga atatcgcccg atttaaaatc agccagcgca
     2761 cgagtacgcg caccttgcga tttattgccg tggatcgccg cactacggat gccatcttta
     2821 ttgagctgtt cagccagatg gttagcgccg tgtttggtac gggtaaacac cagcacctgc
     2881 tgccagttcc ctttcccaat catgtgcgac agcaattcgc gtttgcgttt cttatcgaca
     2941 aagtgaacgt gctgggtcac ctgatcagac gcggtattgc ggcgtgccac ttcgatttcc
     3001 agcgggttgt gcaacagttt ttccgccagg gctttaatat cgtcagagaa ggtcgcggag
     3061 aataacaggt tctggcgctt cgcaggtagt tttgttaaca cgcgacggat atcgtggata
     3121 aagcccatgt cgagcatgcg gtcagcttca tcgaggacga ggatttcaac ctgatccagc
     3181 ttcactgcat tctgatgttc caggtccagc aaacgtcccg gggttgccac cagcacatca
     3241 acgccgccac gcagtttcat catctgcggg ttaatactca caccaccaaa caccaccagc
     3301 gaacgaatgt tcaggtattt gctgtaatca cggacgtttt cgccaatctg cgccgccagt
     3361 tcacgggtcg gggtaagaat gagcgcacgt accggacgac gccctttggc gtgcggctgg
     3421 cgagtgatca ggtgttgcaa cagcggcagc gtaaagcccg ctgttttgcc ggtgccggtc
     3481 tgggcgctag ccatcaggtc gcggccttcc agcaccgcag ggatcgcctg ctgctgaata
     3541 ggggtgggtt cacggtaacc ctgctcggca acggcgcgca ggatatcagg gcttaaaccc
     3601 aaagaatcga aagacataac tactccgaac cgccccgacc gttaccggtg tagttttcag
     3661 ggagatactg aaaaggaaaa tgacaaaaac cacaatgtca tgagggagcg gagtgtagca
     3721 gtttttgtga cgcagcgcat aaattatccc tgtgactggc gttgctaaaa ttcttagcca
     3781 tactggacaa ctcccctatg gagtcataat cttaatcaat catttgatta agaatggact
     3841 gtgcgatgaa taatcctgtc atgacaatca agggtgaaca ggcgaaaaaa cagctgattg
     3901 ctgccgcact ggcgcagttt ggtgaatatg gaatgaacgc caccactcgc gagatagccg
     3961 cccaggccgg gcagaatatc gctgccatca cctactactt cggttcgaaa gaagatttgt
     4021 acctcgcctg cgcccagtgg attgccgatt ttattggcga gcagttccgt ccgcatgccg
     4081 aggaagccga acgcttgttc gcgcaaccac agcctgatcg ggctgccatc cgtgaactga
     4141 tccttcgcgc ctgcaggaac atgattaagc tgctcaccca ggatgacacc gtcaacctca
     4201 gcaagtttat ctcccgtgag cagctctctc ccacggcagc ctaccacctg gtgcacgaac
     4261 aggtgattag tccgctacac agccacctga cacgcctgat tgccgcctgg accggctgcg
     4321 acgccaatga cacccgcatg atccttcata cccatgcgtt gattggtgag attctggcgt
     4381 tccgtcttgg caaagaaaca attctgttac gtaccggctg gaccgcgttc gatgaagaaa
     4441 agaccgaact gatcaaccag acggtgacct gtcatatcga cctgattttg caaggattat
     4501 cgcaaaggag tttgtagtga tgaaaaaacc tgtcgtgatc ggattggcgg tagtggtact
     4561 tgccgccgtg gttgccggag gctactggtg gtatcaaagc cgccaggata acggcctgac
     4621 gctgtatggc aacgtggata ttcgtacggt aaatcttagt ttccgtgttg gggggcgcgt
     4681 tgaatcgctg gcggtggacg aaggtgatgc tatcaaagcg ggccaggtgc tgggcgaact
     4741 ggatcacaag ccgtatgaga ttgccctgat gcaggcgaaa gcgggtgttt cggtggcaca
     4801 ggcgcagtat gacctgatgc ttgccgggta tcgcgatgaa gaaatcgctc aggccgccgc
     4861 agcggtgaaa caggcgcaag ccgcctatga ctatgcgcag aacttctata accgccagca
     4921 agggttgtgg aaaagccgca ctatttcggc aaatgacctg gaaaatgccc gctcctcgcg
     4981 cgaccaggcg caggcaacgc tgaaatcagc acaggataaa ttgcgtcagt accgttccgg
     5041 taaccgtgaa caggacatcg ctcaggcgaa agccagcctc gaacaggcgc aggcgcaact
     5101 ggcgcaggcg gagttgaatt tacaggactc aacgttgata gccccgtctg atggcacgct
     5161 gttaacgcgc gcggtggagc caggcacggt cctcaatgaa ggtggcacgg tgtttaccgt
     5221 ttcactaacg cgtccggtgt gggtgcgcgc ttatgttgat gaacgtaatc ttgaccaggc
     5281 ccagccgggg cgcaaagtgc tgctttatac cgatggtcgc ccggacaagc cgtatcacgg
     5341 gcagattggt ttcgtttcgc cgactgctga atttaccccg aaaaccgtcg aaacgccgga
     5401 tctgcgtacc gacctcgtct atcgcctgcg tattgtggtg accgacgccg atgatgcgtt
     5461 acgccaggga atgccagtga cggtacaatt cggtgacgag gcaggacatg aatgatgccg
     5521 ttatcacgct gaacggcctg gaaaaacgct ttccgggcat ggacaagccc gccgtcgcgc
     5581 cgctcgattg taccattcac gccggttatg tgacggggtt ggtggggccg gacggtgcag
     5641 gtaaaaccac gctgatgcgg atgttggcgg gattactgaa acccgacagc ggcagtgcca
     5701 cggtgattgg ctttgatccg atcaaaaacg acggcgcgct gcacgccgtg ctcggctata
     5761 tgccgcagaa atttggtctg tatgaagatc tcacggtgat ggagaacctc aatctgtacg
     5821 cggatttgcg cagcgtcacc ggcgaggcac gtaagcaaac ttttgctcgc ctgctggagt
     5881 ttacgtctct tgggccgttt accggacgcc tggcgggcaa gctctccggt gggatgaaac
     5941 aaaaactcgg cctggcctgt accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac
     6001 ccggcgtcgg cgttgaccct atctcacggc gcgaactgtg gcagatggtg catgagctgg
     6061 cgggcgaagg gatgttaatc ctctggagta cctcgtatct cgacgaagcc gagcagtgcc
     6121 gtgacgtgtt actgatgaac gaaggcgagt tgctgtatca gggagaacca aaagccctga
     6181 cacaaaccat ggccggacgc agctttctga tgaccagtcc acacgagggc aaccgcaaac
     6241 tgttgcgacg cgccttgaaa ctgccgcagg tcagcgacgg catgattcag gggaaatcgg
     6301 tacgtctgat cctcaaaaaa gaggccacac cagacgatat tcgccatgcc gacgggatgc
     6361 cggaaatcaa catcaacgaa actacgccgc gttttgaaga tgcgtttatt gatttgctgg
     6421 gtggtgccgg aacctcagaa tcgccgctgg gtgcaatatt gcatacggta ggaggcacac
     6481 ccggcgagac ggtgatcgaa gcgaaagaac tgaccaagaa atttggtgat tttgccgcca
     6541 ccgatcacgt caactttgcc gttaaacgtg gggagatttt tggtttgctg gggccaaacg
     6601 gcgcgggtaa atcgaccacc tttaagatga tgtgcggttt gctggtgccg acttccggcc
     6661 aggcgctggt gctggggatg gatctgaaag agagttccgg taaagcgcgc cagcatctcg
     6721 gctatatggc gcaaaaattt tcgctctacg gcaacctgac ggtcgaacag aatttacgct
     6781 ttttctctgg tgtgtatggc ttacgcggtc gggcgcagaa cgaaaaaatc tcccgtatga
     6841 gcgaggcatt tggcctgaaa agtatcgcct cccacgcgac cgatgaactg ccattaggtt
     6901 ttaaacagcg gctggcgctg gcctgttcgc tgatgcatga accggacatt ctgtttctcg
     6961 acgaaccaac gtccggcgtt gatcccctca cccgccgcga attttggctg catatcaaca
     7021 gcatggtaga gaaaggcgtc acggtgatgg tcaccactca ctttatggat gaagcagaat
     7081 attgcgaccg catcggcctg gtgtaccgtg ggaaattaat tgccagcggc acgccggatg
     7141 atttgaaagc gcagtcggcc aacgacgagc aacccgatcc caccatggag caagccttta
     7201 ttcagttgat ccacgactgg gataaggagc atagcaatga gtaacccgat cctgtcctgg
     7261 cgtcgcgtac gggcgctgtg cgttaaagag acgcggcaga tcgttcgcga tccgagtagc
     7321 tggctgattg cggtagtgat cccgctgcta ctgctgttta tttttggtta cggcattaac
     7381 ctcgactcca gcaagctgcg ggtcgggatt ttactggaac agcgtagcga agcggcgctg
     7441 gatttcaccc acaccatgac cggttcaccc tacatcgacg ccaccatcag cgataaccgt
     7501 caggaactga tcgccaaaat gcaggcgggg aaaattcgcg gtctggtggt tattccggtg
     7561 gattttgcgg aacagatgga gcgcgccaac gccaccgcac cgattcaggt gatcaccgac
     7621 ggcagtgagc cgaataccgc taactttgta caggggtatg tcgaagggat ctggcagatc
     7681 tggcaaatgc agcgagcgga ggacaacggg cagacttttg aaccgcttat tgatgtacaa
     7741 acccgctact ggtttaaccc ggcggcgata agccagcact ttattatccc gggtgcggtg
     7801 accattatca tgacggtcat cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg
     7861 gaacgcggca ccatggaggc tctgctctct acggagatta cccgcacgga actgctgctg
     7921 tgtaagctga tcccttatta ctttctcggg atgctggcga tgctgctgtg tatgctggtg
     7981 tcagtgttta ttctcggcgt gccgtatcgc ggttcgctgc ttattctgtt ttttatctcc
     8041 agcctgtttt tactcagtac cctggggatg gggctgctga tttccacgat tacccgcaac
     8101 cagttcaatg ccgctcaggt cgccctgaac gccgcgttcc tgccgtcgat tatgctttcc
     8161 ggctttattt ttcagatcga cagtatgccc gcggtgatcc gcgcggtgac gtacattatt
     8221 cccgctcgtt atttcgtcag caccctgcaa agcctgttcc tcgccgggaa tattccagtg
     8281 gtgctggtgg taaacgtgct gtttttgatc gcttcggcgg tgatgtttat cggcctgacg
     8341 tggctgaaaa ccaaacgtcg gctggattag ggagaagagc atgtttcatc gcttatggac
     8401 gttaatccgc aaagagttgc agtcgttgct gcgcgaacca caaacccgcg cgattctgat
     8461 tttacctgtg cttattcagg tgatcctgtt cccgttcgcc gccacgctgg aagtgactaa
     8521 cgccaccatc gccatctacg atgaagataa cggcgagcat tcggtggagc tgacccaacg
     8581 ttttgcccgc gccagcgcct ttacccatgt gctgctgctg aaaagcccac aggagatccg
     8641 ctcaaccatc gacacacaaa aggcgttact actggtgcgt ttcccggctg acttctcgcg
     8701 caaactggat accttccaga ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa
     8761 cagtgcgcaa attgccgcca actacctgca acagatcgtc aaaaattatc agcaggagct
     8821 gctggaagga aaaccgaaac ctaacaacag cgagctggtg gtacgcaact ggtataaccc
     8881 gaatcttgac tacaaatggt ttgtggtgcc gtcgctgatc gccatgatca ccactatcgg
     8941 cgtaatgatc gtcacttcac tttccgtcgc ccgcgaacgt gaacaaggta cgctcgatca
     9001 gctactggtt tcgccgctca ccacctggca gatcttcatc ggcaaagccg taccggcgtt
     9061 aattgtcgcc accttccagg ccaccattgt gctggcgatt ggtatctggg cgtatcaaat
     9121 ccccttcgcc ggatcgctgg cgctgttcta ctttacgatg gtgatttacg gtttatcgct
     9181 ggtgggattc ggtctgttga tttcatcact ctgctcgaca caacagcagg cgtttatcgg
     9241 cgtgtttgtt tttatgatgc ccgccattct cctttccggt tacgtttctc cggtggaaaa
     9301 catgccagta tggctgcaaa acctgacgtg gattaaccct attcgccact ttacggacat
     9361 taccaagcag atttatttga aggatgcgag tctggatatt gtgtggaata gtttgtggcc
     9421 gctactggtg ataacggcca cgacagggtc agcggcgtac gcgatgttta gacgtaaggt
     9481 gatgtaactt cttatctttc gccagcaaag acactaccgc cgggccagca aggatcgcca
     9541 gccctgcaat tgccagcaaa aagttgtttt ccagtacgcg ggacaacagc cacagcacaa
     9601 tcagcgctgc gccaaaatac caggtcgtgg tcagctcttc cagcacgtct gccacctcac
     9661 gccagcgttg ctcggggtgg cgctgaaaca ccagcatcat cactaccgtg acacaataca
     9721 gtgcgcataa tcccacgccg acgtgaacca gagtcttact catccagccc accaccagca
     9781 gcgccactac cagtaaatgc aacataatct gccagcaact tagacccgtt gcgacacgaa
     9841 cacgttgttg ccacttcatg gcttctctcc tgaagaattt atctctactt actcagagta
     9901 ccgaacttta cggaaaattc cgtaagaccg cacctttttg tgacgccgac tacactattt
     9961 caatgagata agaggcaaag acaggaaaat tatgcccgat caaacacaac aattttcgtt
    10021 caaggtgctc accatcaata ttcacaaagg ctttaccgcg tttaaccgac gcttcatttt
    10081 gccggaactt cgcgatgccg tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt
    10141 gatgggcgcg cacgaagttc atccgctgca tgtggaaaac tggcccgata cctcgcacta
    10201 cgagtttctc gccgacacta tgtggagcga ttttgcctac ggtcgcaatg ccgtataccc
    10261 ggaagggcat cacggcaacg ccgtactgtc gcgttatccc attgaacatt atgagaatcg
    10321 cgatgtttcg gtcgatggtg cggaaaagcg cggcgtgctc tactgccgca ttgtgccgcc
    10381 gatgaccgga aaagcgattc atgtgatgtg cgtacatctg ggcctgcgtg aggcgcaccg
    10441 tcaggcgcag cttgcgatgc tcgccgaatg ggtcaatgag ctgccggacg gcgaaccagt
    10501 attggtggcg ggtgatttca acgactggcg gcaaaaagct aatcatccgt taaaagtgca
    10561 ggccgagctg gatgagatat ttacccgcgc ccacggacgc ccggcgcgca cgtttccggt
    10621 gcaatttcct ctactacgtc tggacaggat ctacgtcaaa aatgccagcg ccagcgcgcc
    10681 aaccgcgttg ccgctgcaga catggcgaca cctttctgat catgcccctt taagtgcgga
    10741 gattcattta tgaaatgtag ctggcgtgaa ggcaataaga tcgagttgct ggaaaacggc
    10801 gagcaatatt atcccgcggt gtttaaggcg attggcgagg cacaagaacg catcattctt
    10861 gaaacgttta tctggtttga ggatgacgtc ggcaaacagc tgcatgcggc actactggca
    10921 gcagcgcaac gcggggttaa agcggaagtc ttgctggatg gctacggttc gccggatctc
    10981 agcgatgagt ttgtcaatga actgacggca gctggcgtgg tatttcgcta ctacgatccc
    11041 cgccctcgcc tttttggtat gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg
    11101 gtgatcgacg cgcgtatcgc ctttattggc gggctgaatt actccgccga gcatatgtcc
    11161 agctacggtc cagaggctaa acaggattac gcggtacgcc ttgaagggcc gattgtcgaa
    11221 gatattctcc agtttgagct ggaaaacctg cctggacaga gcgcggcacg acgctggtgg
    11281 cgacgtcatc acaaagcgga agagaaccgc cagccgggag aagcgcaggt attgctggtc
    11341 tggcgcgata acgaagaaca tcgcgatgat attgaacgtc actatctgaa aatgctcact
    11401 caggcgcagc gagaagtcat tatcgccaac gcctacttct tccccggcta tcgattttta
    11461 cacgccttgc gtaaagcggc acggcgcggg gtgcggatca aactgatcat tcagggcgaa
    11521 ccggatatgc cgattgtcag agtcggtgcg cgcttgctgt ataactatct ggttaaaggc
    11581 ggcgttcagg tatttgagta ccgccgccgc ccgctccacg gcaaagtggc attgatggac
    11641 gatcactggg cgacagtagg gtccagtaat ctcgatccgc tcagtttgtc actgaatctc
    11701 gaagcaaatg tcatcatcca cgatcgtcat tttaaccaga cgctgcgcga taatctgaac
    11761 ggcattattg ccgcagattg tcagcaggtc gatgagacca tgctgccgaa acgcacctgg
    11821 tggaacctga ccaaaagcgt gctggcgttc cactttttac gccacttccc ggcgctggtt
    11881 ggctggcttc cggcacacac accacgtctg acgcaggttg atccccccgc acaaccgaca
    11941 atggaaacgc aggatcgggt agaaactgaa aacacggggg taaaaccctg atgagtaaat
    12001 cacacccgcg ctggcgctta gcaaagaaga tcctcacctg gctgtttttt atcgcggtga
    12061 ttgtgttgct ggtggtctac gccaaaaagg tggactggga agaggtctgg aaggtcatcc
    12121 gtgactacaa tcgcgttgcg ctgctcagtg cggtcgggct ggtggtcgtc agttatctga
    12181 tttacggctg ctatgacctg ctcgcccgct tttactgcgg tcacaaactg gcgaagcgcc
    12241 aggtgatgct ggtgtcgttt atctgctacg ccttcaacct gacactcagt acctgggtcg
    12301 gcggcattgg tatgcgctat cgtttgtact ctcgtctggg gttgcccggt agcactatta
    12361 cgcggatttt ctcgctcagt atcaccacca actggctggg ctatattttg ctggcgggga
    12421 ttatctttac cgcaggcgtg gtggagttgc cggatcactg gtatgtcgat caaactacgc
    12481 tgcgtattct cggcattggc ttactgatga ttatcgcggt ttatttgtgg ttttgcgctt
    12541 tcgcgaagca ccgccatatg accattaaag ggcaaaagct ggtactgcct tcatggaaat
    12601 tcgccctcgc ccagatgctg atttccagcg ttaactggat ggtaatgggg gcgattatct
    12661 ggctgttact tggtcaaagc gttaactatt tctttgtact gggcgtgttg ctggttagta
    12721 gtattgcagg cgtcatcgtg catattccgg cgggaattgg tgtgctggaa gcggtgttta
    12781 tcgcgctact ggctggggag catatcacca aaggcacaat tatcgccgcc ctgctcgctt
    12841 accgtgtgct gtattacttt atcccactgc tgctggcgct gatctgttac ctgttgctgg
    12901 aaagccaggc gaaaaagcta cgagcgaaaa atgaagcggc gatgtgaagt tcagttaagt
    12961 tctttagtat ctgcatttac gactaatgaa aaaaacgcgt atgcctttgc cagacaagcg
    13021 ttatagctat cgcggcaagt atcccaaaga agccgataaa atagtaatag agtgttaatg
    13081 cacctgtaaa agcgcaacga accggactcc acaactcttc actatggcat tttcctgcca
    13141 gttcaaggag cttatttcgt ccatgcgaga taatgcctgg ccataacaca atcattaagc
    13201 aacttattac ccaaatgggc cgttcactca ttaatgccgc attcacgatg attaccaggg
    13261 ccaaccctgt caacgtcatc ataatgataa aacggtgaga accaggatca acattaaata
    13321 ataaaccggc cagcatactg attaaaaaca tcgcgcccgc agtgctgaac acgatgataa
    13381 tggcatatac aggaactaaa cttgcaacga caatcgccac caaagagagt aaaaacagat
    13441 atattacgcc cgcgatggcg gcatttatct tgttgatatc cttacttaag ccatacatac
    13501 aaagtaataa taaccctatg gcgacataca ttggtggtga atcatatttg gttgccgtaa
    13561 tttcgagata cttagctccc caggctagtc ctgcggttgc caccaaacct aaagcggtaa
    13621 tgagccatat accgtttaat atcctggcct cgtgctgaaa atctaattta ttgctgtttt
    13681 tcgagtatga ttccattctt ttagttatcc ttagaaaaaa tattaatatt catcatgaat
    13741 aaatttaaac ccacgcatac aaaatacttt tgtatttatg gatacggaaa acgcttccgc
    13801 tcgtccataa ggcgtatttt atacctggta cttgttaagt gtcgttttta gtaagaaaaa
    13861 aaatcccgac acatggccgg gatattgatg gtgaaaaaga attaacggcg gttgccgaag
    13921 atccgcagca acatcaggaa caggttgatg aagtccagat acaaggttaa cgcgccaaga
    13981 atggaatatt tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc catatttttc
    14041 agtttctggg tgtcatacgc cgtcaatccg acaaagacaa tcacgccgat gtaggtgact
    14101 gcccacatca acgcttcgct tttcagccag aagttgacca gcgatgccag cacaatgccg
    14161 attaacgcca taaacagcat attgccaaag ccgcttagat cgcgcttcgt ggtgtaacca
    14221 tacaggctca ttgcgccgaa catcccggca gtaacgacga aagtactggc gatagaagca
    14281 gcggtataga caatgaaaat actggaaagc gtaagacccg tcagcgccga ataaagcata
    14341 aagagcatag tcgttacacc tgcgctcagc ttttgaatca tcgctgataa cacaataacc
    14401 aatgctaatt gcgcgatgat cagaccgatt aaaaagacac ggttagtgaa caacagctcc
    14461 atcactgccg cagaattagc tgcataccag gcaacaaatg cggtcagcaa caagccaacg
    14521 gtcatccagc catagacttg cgccatataa gtctgcaagc cagcccgggg ttgtacaatt
    14581 gaatcagaac gtgggaatct gtccatgacg atctcctgaa gatattagga atatcttaag
    14641 gatactgcaa aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg
    14701 aaattgccgg acgcagcggt gccttatccg gctaacaaaa aactaccagc gttttgccgc
    14761 ctgccgatcg ctctcccgag cttcgaccca gcggtcgcct tccggcgtgg cttcacgctt
    14821 ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact
    14881 gctgcgatgc gcactggtga caccgacaaa aacgatttca tcgcccggcc ataattcccc
    14941 gatgcggtga atcacagtga cgcgccccag cggccagcgg ttacgcgctt catcaacaat
    15001 ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg cgttgacgct
    15061 gtcgcccagg ttatggttgc gcaccttacc agtaaaggtg actaccgcac cgtcttcgtc
    15121 acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcggac caacaacaat
    15181 tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagctact tcgtcgccgt
    15241 cagtcagcgg atggtcaaaa ctcaccagcg tctggttgac ggcagccagt aatttgccat
    15301 cttccagcgc cagcgcccag cgatcgctct gcgcagccat gtgctggcgt aacgcttcaa
    15361 cagttgggaa atccgcagcc acttcggttg catctgttcc caccaactcg cgcacctggg
    15421 cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc
    15481 ttcgccagca aacgtaccga accaatcacc atatcttttt gcaccgcttt gcacatgtca
    15541 taaatggtca gcgccgccac ggaggccgcg gttaatgctt ccatttcgac accggttttc
    15601 ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa
    15661 ttgacttcaa ctttgctgag catcagcgga tgacagagcg ggatcagatc ccaggtgcgt
    15721 tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga
    15781 ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct
    15841 tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg
    15901 atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg
    15961 tacgggcatc caactgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg
    16021 aacccggcat ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca cgagattgca
    16081 acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa
    16141 cttcacggtc gaacaacggc agcaatgctt cgggagcctg atcaccttcc gtcaggccag
    16201 taccacccgt aatcaacacc acttgtacat cgtcgctggc gatccacgca gatacctgag
    16261 cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga tggcccgctt
    16321 cttgcgccga atcgcgcaaa tagtgaccgg aggtatcgtc ttcttcaccg cgccgattag
    16381 aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat
    16441 ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt
    16501 tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca
    16561 gcgcctgttg ctgggcatcg tcttccagca gatcgcgcag gttaacgccg ccttcaccaa
    16621 acaggcagag atggagttta ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga
    16681 agtctttttc atacggcatg ataaggccaa tctctccggc gtaatccgga tggcaaaaga
    16741 cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct
    16801 cgtcacgcag aacctgacca gagatgtgat gcttacggaa gagctcactg ccctcgcccg
    16861 tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg
    16921 tgtcgagctg gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa
    16981 aaccggcctc aaatgcagca tcaatccctg ccatgacctg gttgaattta tcctgcccgg
    17041 taatagcgtg aaactggcgg gcgtccagac tgtcgacact gacgttaatg ccagtaagtc
    17101 ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa
    17161 tctggcggat agcgtcgttt tcccgcacag cggcgatgat atcg
//
LOCUS       DACAPB010000090        17112 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.090, whole genome shotgun sequence.
ACCESSION   DACAPB010000090 DACAPB010000000
VERSION     DACAPB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.090"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..551)
                     /locus_tag="IT904_002665"
     CDS             complement(<1..551)
                     /locus_tag="IT904_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAP3192430.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSL"
     gene            783..1664
                     /gene="yddG"
                     /locus_tag="IT904_002666"
     CDS             783..1664
                     /gene="yddG"
                     /locus_tag="IT904_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAP3192431.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1923..2213
                     /locus_tag="IT904_002667"
     CDS             1923..2213
                     /locus_tag="IT904_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP3192432.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            2237..3568
                     /locus_tag="IT904_002668"
     CDS             2237..3568
                     /locus_tag="IT904_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAP3192433.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTE
                     EIVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            3892..5280
                     /locus_tag="IT904_002669"
     CDS             3892..5280
                     /locus_tag="IT904_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAP3192434.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVLVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            5362..9102
                     /locus_tag="IT904_002670"
     CDS             5362..9102
                     /locus_tag="IT904_002670"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP3192435.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNLPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNDFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEDRNQVQEAKK"
     gene            9099..10643
                     /gene="narH"
                     /locus_tag="IT904_002671"
     CDS             9099..10643
                     /gene="narH"
                     /locus_tag="IT904_002671"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP3192436.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            10643..11338
                     /gene="narW"
                     /locus_tag="IT904_002672"
     CDS             10643..11338
                     /gene="narW"
                     /locus_tag="IT904_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP3192437.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            11335..12015
                     /gene="narI"
                     /locus_tag="IT904_002673"
     CDS             11335..12015
                     /gene="narI"
                     /locus_tag="IT904_002673"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP3192438.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            12094..12987
                     /locus_tag="IT904_002674"
     CDS             12094..12987
                     /locus_tag="IT904_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HAP3192439.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVAVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(13083..13928)
                     /gene="nhoA"
                     /locus_tag="IT904_002675"
     CDS             complement(13083..13928)
                     /gene="nhoA"
                     /locus_tag="IT904_002675"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HAP3192440.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDNQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            14101..14670
                     /locus_tag="IT904_002676"
     CDS             14101..14670
                     /locus_tag="IT904_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP3192441.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMASAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(14674..14901)
                     /gene="pptA"
                     /locus_tag="IT904_002677"
     CDS             complement(14674..14901)
                     /gene="pptA"
                     /locus_tag="IT904_002677"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HAP3192442.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(14952..15724)
                     /locus_tag="IT904_002678"
                     /pseudo
     CDS             complement(14952..15724)
                     /locus_tag="IT904_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(16640..16852)
                     /locus_tag="IT904_002679"
     CDS             complement(16640..16852)
                     /locus_tag="IT904_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HAP3192443.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDILGVGDAININTNKNIRQVC"
     gene            complement(16928..>17112)
                     /locus_tag="IT904_002680"
     CDS             complement(16928..>17112)
                     /locus_tag="IT904_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP3192444.1"
                     /translation="AEPYAFGEQLTLVDCYLCTMRTWGPGHEWFQDNAQNISAIADAV
                     CQLPKLQEVLKRNEII"
BASE COUNT         4420 a         4166 c         4058 g         4468 t
ORIGIN      
        1 agggagcggg caaatttctg ggtcagcatc ccggtttcgt tgctggcacc ggaagcacac
       61 agcataccgg tagaaagcca acggtttacc gttacgccct gctcgttctt ttcaataaag
      121 ttagcgtcac ggtcagcttt catcagcttc gcaatacggg agaatgcttc ttcccagcta
      181 atgcgctgcc atttgtcaga acctggcgca cgatattccg ggtagcgcag acggttttcg
      241 ctgttgacgt aatccagcaa accggcccct ttcgggcaca gcgcaccacg gcttaccgga
      301 tgatccggat caccttcaat gtgataaatc gcttctctgg cgtttttcgc gccatcaccc
      361 aggctataca tcaatagccc gcaacctacg gaacagtatg tgcaggtgtt acggatctct
      421 ttagcgcgta ataatttgta gtttcgcgcc tgagccagtg cttgcttcgg ggcaaagccc
      481 aatgccgcta ctgttgttcc agccataccg cccgcgcaga ttttaaaaaa ttgtctgcga
      541 ctgacgtcca ttgctttcct cttttttcag gggtattact gcgcgaggaa actaacagta
      601 aaattctgaa tctttttgcg cgaaatcaaa aagaacacgt gtttgccaca aaataatccg
      661 cctgcgcaaa ttgacctacc tcaatagcgg tagaaaaacg caccactgcc tgacaggcca
      721 gttaaaaaaa tgctataaaa ttcagcttaa tttttaacgg caagagagac aaaacagcga
      781 gcatgacacg acaaaaagca acgctcatag ggctaatagc gatcgtcctg tggagcacga
      841 tggtaggatt gattcgcggt gtcagtgagg ggctcggccc ggtcggcggc gcagctgcta
      901 tctattcatt aagcgggctg ctgttaatct tcacggttgg atttccgcgt attcggcaaa
      961 tcccgaaagg ctatttactc gccgggagtc tgttattcgt cagctatgaa atctgtctgg
     1021 cgctttcctt agggtatgcg gcgacccgtc atcaggcgat tgaagtgggt atggtgaact
     1081 atctgtggcc cagcctgaca attctctttg ccattctgtt taatggtcag aaaaccaact
     1141 ggctgattgt acctggatta ttattagccc tcgtcggcgt ctgctgggtg ttaggcggtg
     1201 acaatgggtt acattatgat gaaatcatca ataatatcac caccagccca ttgagttatt
     1261 tcctggcgtt cattggtgcg tttatctggg cagcctactg cacagtaacg aataaatacg
     1321 cccgcggatt taatggaatt accgtttttg tcctgctaac gggagcaagt ctgtgggttt
     1381 actattttct cacgccacaa ccagaaatgg tatttagcac acccgtcatg attaaactca
     1441 tctctgcggc atttacctta ggatttgctt atgctgcatg gaatgtcggt atattgcatg
     1501 gtaacgtcac cataatggcg gtaggttcgt attttacgcc cgtactttcc tcagcgcttg
     1561 cagccgtgct gctcagcgcc ccgctgtcgt tctcgttctg gcaaggcgcg ctaatggtct
     1621 gcggcggttc cctgctctgc tggctggcga cacgtcgtgg ttaaaataat tgccgggtct
     1681 acggcctggc agttaatcag ataaattccg acaatattaa aaaataaatt tcgaatacca
     1741 gtaattcacc ttatatctca tttcgacttc gatcacatat cgataaataa tattatttca
     1801 tctatttttc tcattgtttt taatcaccat tctaatttaa aaacagtatg tcatttataa
     1861 atgacgccgt ttaaaattcg tttagaaaat acgtttaacg ataattaaaa ggattctgtt
     1921 aaatgaaatt aaaaatagtt gcggtggttg taactggttt gttagctgcg aacgtagcac
     1981 acgctgccga agtctataac aaggatggta ataaactcga cctttatggc aaggttaccg
     2041 ctctacgtta ttttactgat gataagcgtg acgatggtga taaaacttat gcccgtctcg
     2101 gctttaaagg agaaacgcaa atcaatgatc aaatgattgg ttttggtcac tgggaatatg
     2161 attttaaagg ctataacgat gaagccaacg gctcgcgcgg caagaatctc tgattgagtt
     2221 aagtgcccat cacaatatga tcactgacct tatattacac aatcatccca ggatgaaaac
     2281 aatcacttta aacgacaacc atattgcaca tttaaacgcc aaaaacacta caaaactgga
     2341 atatttaaac ttaagcaata acaatttact gccaaccaat gacattgatc aactaatatc
     2401 atcaaaacat ctttggcatg tattagttaa cggcatcaac aatgatccac ttgcacaaat
     2461 gcagtactgg actgcagtaa gaaatataat tgatgacact aatgaagtga ccattgattt
     2521 atcaggactt aatttaacca ctcaaccacc agggctgcaa aacttcacct ctatcaatct
     2581 tgataataac caactcacac attttgatgc aaccaactac gatagactcg taaagctaag
     2641 tctgaatagt aatactcttg agtcaataaa ttttcctcaa ggcagaaatg taagtattac
     2701 acatatatct atgaataata atgctctcag aaatattgat atagataggc tttcatcagt
     2761 tacttatttt agtgcggcac ataatcaact agagtttgtg caattagaat cttgcgaatg
     2821 gctgcaatac ctgaatctca gccataatca attaactgat attgttgcag gtaataaaaa
     2881 tgaactctta ctgctggatc tatcccataa taaactaaca agtttacaca atgacttatt
     2941 tcccaacttg aatacgttac ttattaacaa caatttgctt tctgaaatta aaatattcta
     3001 tagcaacttc tgcaatgttc agacattaaa cgctgctaac aaccagttga aatatataaa
     3061 tcttgatttc ctgacttatc ttccatctat caaaagttta agactggaca ataataaaat
     3121 aacccacatt gatactaata atacatccga tattggaact ttattcccca taataaaaca
     3181 gagcaaaaac ttaaattttt taaatgtttc tgggaagaac aattgcccta ctatgcagct
     3241 catgttattt aatttatttt ccccagcact taagcttaat actggcctgg caattctttc
     3301 gcctggtgca tttgaagttc actctgacgg aatagatgtg gataacgaat tgtttcacta
     3361 tcctattaaa aaagcatata ccccatataa tatacacact tacaagacag aggaaattgt
     3421 aaaccagagg aatataaaag ttaaaaacat gaccttagat gaaataaaca atacttactg
     3481 taataacgat tattacaatc aggcaataag agaggaaccg atagaccttc tggacagatc
     3541 gttttcctcc agttcatggc ctttttagtg aataaatcat cactaattct ataagtgtct
     3601 ggtcttgttg ataatatagt taatatgtaa ccctttagat tcacgatctg aagggttgaa
     3661 acatgacata cgttcaaaga ttaacccccg tttgaaggtt agcttgttag ttacatttag
     3721 taacacatat ctggctactt aatccccatt ctgcccccgc ggttacgctt tcttatctat
     3781 tctttcaatc agcacttcac ccaattcatc cttaattcta ataactggct attgcgcctt
     3841 acctcctgtt ttgtcttttt tcattaccaa tgtgtgcatg tgaggaacaa tatggcactg
     3901 caaaatgaga aaaatagtcg ttatctttta cgcgactgga aaccagaaaa tccggccttc
     3961 tgggaaaata aaggaaaaca tattgctcga agaaacctct ggatatcagt cagttgtcta
     4021 cttcttgcct tctgtgtctg gatgctattt agcgcagtta ccgttaatct caataaaatc
     4081 ggttttaatt ttactaccga tcaactcttt ttattaaccg cattaccctc cgtttctggc
     4141 gcattattgc gtgttcccta ctcctttatg gtgcctatat tcggtggacg ccgatggacg
     4201 gtttttagta ctgcaatcct gattattcct tgcgtctggc tcggaattgc cgtgcaaaat
     4261 ccgaatactc cttttgggat atttatcgtt atcgctttgc tatgcggttt tgcaggtgca
     4321 aactttgctt cgagcatggg caatatcagt ttcttctttc caaaagccaa acaagggagc
     4381 gctcttggga ttaatggcgg attaggaaac ttaggtgtaa gtgtaatgca gctggttgca
     4441 ccgctggtca tttttgtacc tgtatttgcc tttctcggcg tcaatggcgt accgcaggcc
     4501 gacggttcgg tgatgtcgct ggcgaatgcc gcatggattt gggtaccgct actggcgatt
     4561 gccacgatcg ccgcatggtc agggatgaat gatatcgcca gttcacgcgc ctcaattgcc
     4621 gaccagctcc ctgtcttaca acgcctgcat ctctggctgc tgagcctgct ttaccttgcc
     4681 accttcggtt cgtttatcgg tttttctgcg ggttttgcca tgctggcaaa aacccagttc
     4741 ccggatgtga atattctgcg cctggcgttc tttggcccat ttatcggtgc catcgcgcgg
     4801 tcggttggtg gtgctatttc cgataagttc ggcggcgtgc tggtgacgtt gatcaacttt
     4861 atttttatgg cgattttcag tgccctgctg ttccttacct taccgggcac aggctccggt
     4921 aatttcatcg ccttttacgc cgtatttatg gggctgtttc tgactgcggg tctgggaagt
     4981 ggttctactt tccagatgat cgccgtcatc tttcgccaga taaccattta tcgggtaaag
     5041 atgaaaggcg gtagtgatga gcaagctcag aaagaagccg tcaccgaaac ggcggcggct
     5101 ctgggcttta tctcagccat tggcgcagtg ggcggctttt ttattccgca ggcgtttggc
     5161 atgtcgctca atatgaccgg ctctccggtc ggcgcgatga aagtgttttt aatcttctac
     5221 atcgtttgtg tgctgctgac ctggctggtt tatggtcggc ggaagttcag ccaaaaataa
     5281 gagatcgatg cctgatgtac aaccgtgtcg catccgacaa ggcatcagat cccaattcat
     5341 tcctggagca ggagtcatgt catgagtaaa cttttggatc gctttcgcta cttcaaacaa
     5401 aagggcgaaa cctttgccga tggtcacgga caggtgatgc atagcaaccg cgactgggag
     5461 gacagctatc gccagcgttg gcagttcgac aaaatcgtgc gttccaccca cggtgttaac
     5521 tgtacaggct cctgtagctg gaaaatctac gttaaaaatg gtctggtgac ctgggaaatc
     5581 caacagaccg actacccgcg cactcgccct gacctgccca atcatgaacc tcgcggctgc
     5641 ccgcgtggcg caagttactc ctggtatctt tacagcgcta accgcctgaa atacccgctc
     5701 attcgtaaac gactgattga actgtggcgc gaagccctca agcaacacag cgatccggta
     5761 ctggcgtggg catcgattat gaacgatccg caaaagtgcc tgagctacaa acaagtgcgt
     5821 gggcgcggcg ggtttatccg ctccaactgg caggaactaa accagctgat tgccgccgct
     5881 aacgtctgga ccatcaaaac ctacggcccg gatcgcgttg ccggtttctc gccgatcccg
     5941 gcgatgtcga tggtttctta cgccgccgga acgcgttatc tgtcgctgct tggcggcacc
     6001 tgtttaagtt tctacgactg gtattgcgac ctgccgcccg cctcgccgat gacctggggc
     6061 gagcaaaccg acgtaccgga atctgccgac tggtataact ccagctacat catcgcctgg
     6121 gggtctaacg taccgcagac acgtacgccg gacgcccact tctttaccga agtacgctac
     6181 aaaggcacta aaaccatcgc cattacccct gactactctg aagtggccaa attgtgcgac
     6241 cagtggctgg caccgaaaca aggcactgat agcgccctgg cgatggcaat gggccatgtg
     6301 attttaaaag agtttcatct cgataatccc agcgactact ttatcaacta ctgccgccgc
     6361 tacagcgaca tgccgatgct ggtaatgctg gagcctcgcg acgatggtag ctacgttccc
     6421 gggcgcatga tccgcgcatc tgacctggtg gatggactgg gcgaaagcaa caatccgcag
     6481 tggaaaaccg tagcagttaa taccgcaggt gaattggtag tgccgaacgg ttcgattggt
     6541 ttccgctggg gagaaaaagg caaatggaat ctggaatcca ttgccgccgg tacggaaacc
     6601 gaattgtcgt taaccctgct cggtcaacat gacgctgttg caggcgtggc cttcccctac
     6661 tttggcggca ttgaaaatcc gcattttcgc agcgtaaaac acaatccggt gctggtgcgc
     6721 caattgcccg ttaaaaacct gacgttagtc gatggcaaca cctgtccggt ggtcagcgtt
     6781 tatgatttgg tactggcgaa ttacggcctc gatcgcgggc tggaagatga aaacagtgcg
     6841 aaagattacg ctgaaatcaa accgtacacc ccagcctggg gtgagcaaat taccggcgtg
     6901 ccgcgccagt atattgaaac catcgctcgt gaatttgccg atactgccca taaaacgcat
     6961 gggcgctcaa tgattatcct cggtgctggc gtcaaccact ggtatcacat ggatatgaac
     7021 taccgtggga tgatcaatat gctgatcttc tgcggttgtg tcgggcaaag cggcggcggc
     7081 tgggcgcatt atgtcggtca ggaaaaactg cgcccacaaa ccggctggtt gccgctggcc
     7141 tttgcgctcg actggaacct cccaccgcgc cagatgaaca gtacctcgtt tttctacaat
     7201 cattccagcc aatggcgcta tgaaaaagtc tctgcgcagg agttactttc accgctcgcc
     7261 gatgccagta agtacagcgg tcatctgatt gatttcaacg ttcgcgccga acgtatgggc
     7321 tggctgccct ctgcgccgca gctggggcgt aacccgctcg ggattaaagc tgaagccgac
     7381 aaagcaggat tatcccccac agaatttacc gcccaggcgc tgaaatcggg cgatttacgt
     7441 atggcctgcg aacaaccaga tagcggcagc aatcatccgc gtaatttgtt tgtctggcgt
     7501 tctaacctgc ttggctcctc cggcaaaggc cacgagtata tgcagaagta tctgctgggg
     7561 accgaaagcg ggattcaggg cgaggaactc ggtgccagcg acgggatcaa accggaagaa
     7621 gtcgagtggc aaactgcggc gattgaaggc aagctcgacc tgctggtgac gctcgacttc
     7681 cgcatgtcca gcacctgcct gttctccgat attgttctgc ctaccgccac ctggtacgaa
     7741 aaagacgata tgaatacctc ggatatgcat ccgtttattc atccactttc tgcggcagtc
     7801 gatccggcct gggagtcacg cagcgactgg gaaatctaca aaggtattgc caaagcattt
     7861 tcgcaagtgt gcgtgggtca tcttggcaaa gaaaccgacg tggtattaca accactgctg
     7921 cacgactctc cggcagagct ctcacagccg tgtgaagtcc tcgactggcg caaaggcgaa
     7981 tgcgatctga ttccaggtaa aaccgcaccc aatattgtgg cggtggagcg cgactaccct
     8041 gctacctatg aacgctttac ctcgctcggg ccattgatgg acaaacttgg caacggcggt
     8101 aaagggattt cgtggaatac gcaggatgaa atcgatttcc tcggtaaact caattacacc
     8161 aagcgtgatg gcccagcgca ggggcgtccg ctgattgaca ccgccattga cgcttcagaa
     8221 gtgattctgg cactggcacc agaaacaaac ggtcatgttg cagttaaagc gtggcaggcg
     8281 ctgggcgaga tcaccggacg cgaacatacc catctggcgc tgcacaaaga ggacgagaag
     8341 attcgctttc gcgatattca ggcgcagcca cgtaaaatta tctccagccc cacatggtct
     8401 ggtctggaaa gcgatcacgt ctcctataac gcgggataca ccaacgttca tgagttaatt
     8461 ccgtggcgca cgctgtcggg acgccagcag ctctatcagg atcatccgtg gatgcgtgct
     8521 tttggtgaaa gcctggtggc ttatcgcccg cctatcgaca cccgtagcgt cagtgagatg
     8581 cgccagatac cgccaaacga cttcccggaa aaagcactta acttcctgac gccgcaccag
     8641 aaatggggca ttcactcaac ctacagtgaa aacctgctaa tgctgacgct ctctcgcggt
     8701 ggaccgattg tctggatcag cgaaaccgat gcccgagaac tgaccattgt cgataacgac
     8761 tgggtggaag tgtttaacgc caatggcgcg ctgacggccc gcgcggtggt cagccaacgt
     8821 gtaccgccgg gtatgaccat gatgtatcac gctcaggaac gcattatgaa tattcctggt
     8881 tcggaagtaa ctggcatgcg cggcggtatt cataactcgg ttacccgcgt ttgcccgaaa
     8941 ccaacgcata tgattggcgg ttacgcgcag ctggcctggg gctttaacta ctacggcacc
     9001 gtcggatcga accgcgatga gttcatcatg atccgcaaga tgaagaacgt taactggctg
     9061 gatgatgaag atcgcaatca ggtacaggag gcgaaaaaat gaaaatacgt tcgcaagtcg
     9121 gcatggtgct taacctcgac aaatgtatcg gctgccatac ctgttcggtg acctgtaaaa
     9181 acgtctggac cggacgcgaa ggcatggagt acgcatggtt taacaacgtc gaaaccaaac
     9241 cgggcattgg ttatccgaaa aactgggaag atcaggaaga gtggcaaggc ggctgggtcc
     9301 gcgatgtgaa tggtaagata cgcccgcgtc tgggtagcaa gatgggcgta ataaccaaaa
     9361 tcttcgccaa cccggtggtg ccgcagattg atgattacta cgaacctttc accttcgact
     9421 acgaacattt gcatagcgca ccggaaggca aacatatccc tactgctcgc ccgcgttcgc
     9481 tgattgacgg taagcggatg gacaaagtga tctgggggcc aaactgggaa gaactgctgg
     9541 gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga ggccatgcaa aaggagatgt
     9601 acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg cctgtgcgaa cactgcctca
     9661 atcccagttg cgtggcgacc tgcccaagcg gcgctatcta caaacgcgaa gaagacggca
     9721 ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt gtgcattagc ggttgtccgt
     9781 acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga aaaatgtatc ttctgttatc
     9841 cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac ctgcgtgggt cgcatccggt
     9901 atctgggcgt gctgctttac gacgccgacc gcattgagga agcggcgagc accgagcgcg
     9961 aagttgacct ctatgaacgt cagtgcgaag tgttcctcga tccacacgat ccctcagtga
    10021 tcgaggaagc cctgaaacaa ggtattccac aaaacgtgat tgaagctgcc cagcgttcgc
    10081 ctgtctacaa aatggcgatg gactggaaac tggcgctacc gctgcaccct gaatatcgca
    10141 ccctgccaat ggtctggtac gttcctccgc tgtcgccgat tcagtcctac gcagatgcgg
    10201 gcggtttgcc gaaaagcgaa ggcgtgctgc ccgccatcga aagcctgcgt attccggtgc
    10261 aatatctcgc caatatgttg agtgccggcg ataccggtcc ggtactgcgg gcgctgaaac
    10321 ggatgatggc gatgcgccac tatatgcgtt cacaaaccgt ggaaggcgtt actgatactc
    10381 gtgccatcga cgaagtaggc ctgagcgtcg cccaggtcga agagatgtat cgttacctcg
    10441 ccattgccaa ctatgaagat cgttttgtca tcccgacgag ccatcgggaa atggcgggcg
    10501 atgccttcgc agaacgcaac ggctgcggtt ttacctttgg cgacggttgt cacggctcgg
    10561 acagcaaatt caacctgttc aacagtagcc gtatcgatgc catcaacatc accgaagtgc
    10621 gcgacaaagc ggagggcgaa taatgcagat cctcaaagtg atcggcctgt tgatggagta
    10681 tccggacgag ctgttgtggg aatgcaagga ggacgcgctg gcgttgatcc gccgcgacgc
    10741 gccgatgctt acggatttca cccacaacct gcttaacgcg ccgctgctgg ataaacaggc
    10801 cgaatggtgc gaagtgtttg accgtggacg taccacatcg ctgctgctgt tcgaacatgt
    10861 tcatgccgag tcccgcgatc gcggccaggc aatggtggac ctgctggcgg agtatgaaaa
    10921 ggtcggcctg caactggatt gtcgggaact gcccgattat ctaccgctgt atctggagta
    10981 tttaagcgta ctgcctgacg atcaggcgaa agaaggattg ctcaatgttg cgccgatcct
    11041 cgccctgctt ggcggtcgct taaaacaacg cgaggcaccg tggtacgcgt tgtttgatgc
    11101 tctgctgcaa ctggcgggaa gctccctttc aagtgacagt gtcacaaaac aagtaaacag
    11161 cgaagagcgc gatgacaccc gccaggcgct tgatgcagtg tgggaagagg aacaggtgaa
    11221 gtttattgaa gataacgcca cggcgtgtga cagctcgccg cttaatcaat atcagcgacg
    11281 ctttagccag gatgtcgcgc cgcaatatgt cgacatcagt gcgggaggtg ggaaatgatt
    11341 cagtatctga acgtcttttt ttacgatatc tacccgtaca tttgtgcgac ggtgtttttc
    11401 ctcggcagct ggctgcgcta cgactacggg cagtacacct ggcgcgcctc ctcaagtcaa
    11461 atgctcgata aacgcgggat ggtgatatgg tcgaatctgt tccatatcgg cattttgggg
    11521 attttcttcg ggcacttgtt cggcatgtta acgccgcact ggatgtacgc gtggtttttg
    11581 ccagtggcag cgaaacaact catggcgatg gtgctcggcg gtatttgcgg tgtgttgacg
    11641 ttgattggcg gcgcaggact gctgtggcgc aggctgacaa atcagcgcgt gcgtgccact
    11701 tccactacgc cggatatcat catcatgagc attttgctga tccagtgtct gttggggcta
    11761 agtaccatac cgttttcagc acagtatcct gatggtagcg aaatgatgaa gctggtgggt
    11821 tgggcgcaaa gcattgtcac tttccgtggt ggatcgtcag agatgctcaa cggcgtagcg
    11881 ttcgtcttcc gcctgcatct ggtgttggga atgaccatct tcctgctctt cccgttcacc
    11941 cgactggtgc atgtgtggag cgcgccgttt gagtacttta ctcgtcgata tcaaattgtg
    12001 cgttcgcggc gataatttca ccctggccgg atagcccttc gctctccggc cacttgcagg
    12061 ctatgatagt tctccagtca caggagaacg ttaatgaaac cgcaggtgta ccacgtcgat
    12121 gcttttacct cacaaccgtt tcgcggcaat tctgccggtg ttgttttccc cgccgataat
    12181 cttagcgaag cgcaaatgca gcttatcgcc cgcgagttag gccattcgga aactgctttt
    12241 ctgctgcaca gcgacgacag cgatgtgcgc atccgctact ttacgccaac ggttgaagtg
    12301 ccgatttgcg gtcacgcgac agtagctgcg cactatgtac gtgcgaaggt gttaggttta
    12361 ggaaattgca cgatctggca aacatcgctg gcaggaaaac atcgcgtgac tatcgaaaag
    12421 cacaacgatg attatcgtat ttcgctggaa caaggtacgc cgggctttga actgccgctg
    12481 gagggtgaag tacgcgcggc gattatcaac gcattgcatc ttactgaaga cgatattctg
    12541 ccgggtttgc cgatccaggt cgcgacgacc gggcattcta aagtgatgat cccattgaag
    12601 ccggaagtgg atatcgacgc tctttcgcct gaccttaatg cgctgaccgc tatcagtaaa
    12661 cagattggct gcaatggttt cttcccgttc cagatccgtc caggcaaaaa cgaaaccgat
    12721 ggtcgcatgt tctcgcctgc gattggtatt gtggaagatc cggtgactgg caatgccaat
    12781 ggcccgatgg gtgcatggtt ggtacatcac aacgtattgc cccacgatgg caaggtgctg
    12841 cgtgttaaag gccatcaggg gcgcgcattg gggcgtgacg gcgtgattga agtggcggtg
    12901 acaattcgtg ataaccaacc ggaaaaagtc accatttctg gcgcggcggt gattttgttt
    12961 catgccgaat gggcaattaa actctgaaat cgttgcctga tgcgcttcgc ttatcaggtc
    13021 tacaaccgag ctaatgtatg ccggataagg cgtttacgcc gcatccggca acctgacata
    13081 aattattttc ccgcctccgg gtgcgtatca aacgccgcca tcaccagcgc taactcatcc
    13141 acggtaaagc catgtttcgc atcatccacg cccagaccaa actgttcttg catcacagca
    13201 tataatgacg ccacatccgg tagatttcgc tgctccaccg cgtgcccatt ttcataatgg
    13261 gtaaaatgaa aattggtcag tgtcagcttg ccgccgtccg gcaaatggcg gcacatcagc
    13321 aaatgatggc gaaaatgcga ctgcggccag tgcgccgacc agaaattgcc catcacataa
    13381 tcgctttgtt gctgctcgca gagatcaaaa cggtacatcg actgccagtg ctgatgatga
    13441 ttaaactgca acacccaatc atcaccctcc tgcaacaacc gatactctcc gtgtggcgtg
    13501 gtctgcacga gatcgggaac taaacgaatc ggcgcggtta gcgtctgccc accgaaaccg
    13561 acatcagcaa tccatttttc ctcttccagt tccaccaaca gcaaacgatg ggtgcgcggc
    13621 ggtaatgctg gcggatttga taacactacg cgccctaaca agctgcgaac gttaaacccc
    13681 agctcgcgta acacccgctc aaacacgcca ttctgctcaa aacagtaacc gccacgacgg
    13741 gctatcacca gtttctcttc cagcgattga ttatcaagct gtatttccct cggcagcaaa
    13801 acgtcgaggt tttcaaacgg aatggtgcaa ttgtgtttca ggtgcaatgc acgaagcgta
    13861 tcaatattga ccgcagcagc tcccgaccag ttaatacggg caaaatagtg attcagaatg
    13921 ggcgtcatca ggcgtttccc ttagtggttt tctcaactta taacctggaa tagacgccgc
    13981 accggctatt ttgtgcgatc gcgtacatgc atcacgttcg caaatcgcca atcccgttaa
    14041 tttcgttact tattgcgtta cggactacgc ttaaaaaaac cttcttcgcg aggcgatacc
    14101 atgagccgat tcatccccat agaattacac catgccagcc gtctgttaaa tcatggccca
    14161 acggtcttga tcaccagttt cgatgagcaa tcccagcggc gtaacattat ggcttcagcc
    14221 tggtcaatgc cggtggagtt tgaacctcca cgcgtggcga ttgtggtaga taaatcgaca
    14281 tggaccaggg agttgattga acataacggt aaatttggca tcgttatccc gggcgttgca
    14341 gcaactaact ggacgtgggc ggtgggaagt gtgtcggggc gtgatgaaga taaatttaat
    14401 tgctatggca ttccggttgt gaaaggtccg gtgcttggtt tgccacttgt tgaagagaaa
    14461 tgcctggcgt ggatggagtg ccgcctgcta cctgcgactt ctgcgcaaca aaaatacgac
    14521 acgctgtttg gcgaggtggt atcagcagcg gcagacgcac gggtatttgt cgaaggccgc
    14581 tggcagtttg atgatgataa gctcaatacg ttgcatcatt taggtgctgg cacgtttgtt
    14641 accagcggca agcgagttac ggcgggttaa gcattacatg ctgtaaccag gtttctttat
    14701 caaagcctcc atttggggcg cgatttcggc atcccagata gcttgccaag attctggttg
    14761 aatctgctgt agagcaatgc ttatcgaact gtctttactg ttcagatggc gaataataac
    14821 gtcggtaata tctgcagcaa gtgctgcttt ttgttgttcg tccagttcac gcggaaaaca
    14881 tttaatgtcg atgtgcggca tgattgtgct ccttttgaaa gatgtcagta aaattaacac
    14941 acattgtgaa ttttcagtcc tttccattca aacgtgaaat caataagttc atcagggaca
    15001 tcgcaaacaa tatgaatacg gatttcttct ctgccgtgac tcttttcaca aattgcgtaa
    15061 ctgtcatgct ctggattatt taattctttc agcggaaatt tttcctcaaa ggctttattt
    15121 agccacccct ggtttccttt caccgcgaat aaataatcac ctccctgttt ttgtatcttc
    15181 tctgctatat ctttctggca acccatcgca tcagttgtga tgatttttcc tttaatatcc
    15241 agcatgttaa gaagttctgg gatagctgta atctcattag atttatcatc cgtcttgatc
    15301 tgtccgatga ccagactgtg cattgttgag aacgcactaa tgacatgaat cgcaccctgc
    15361 ggcgactctt atcataagaa tgccggagcg tttttccatc aattgcaatg acgtctttat
    15421 catctgaaga atgacaatca cgcatccagt taataaagca ctcgtgaaat tttgcaggac
    15481 tgatacagga tacaactctg gcaatggtat cgtgaacagg aataccattt tcaaaatcac
    15541 catattgctt caaaaaatcg agatgtgttt ccccaaaatc ctctatatct tcccaaccct
    15601 ctgcaccaga aataacggca caaatagtta acagtagaat atccgataat ttatgttcca
    15661 ctttccaggc ttgtctgtaa tcggggataa tagaaatatg ttccatcaat tttttaagtt
    15721 ccattttgtt ctccttaatt atgtaagaag tatttgatca tgtataagca ataaaaaaca
    15781 gctttaggta ataaggaata tctcaatttt taaacataaa atgcgaatta tttagtacaa
    15841 aaagtgtact tctgatgatc tttccctgca tgtcgggcgg atgagataag cgagtcgaca
    15901 tctggcgtta ccggggaata tcagaaaaat gtcatggtta tgtgatgatt tagtctggga
    15961 ttttacttca gagaaacggg tgtctgttta gtcaacaaaa taatcacaaa cgcaacggat
    16021 gggttctcga cgttattcac gacaggctcg tacattacag cgagtaataa aaaacctgat
    16081 atgcaaagtc aaaccaatat tcataatgtc tttttattca caacaataaa taattcaata
    16141 acaatcattg aaatgaatga tgatttgtat aagtaatata cgctaccgcc ccaccctgat
    16201 aacatgatct gcacattgcc atcttaacgt cgttaaacac tggcttaata gtggatattt
    16261 caaattagga atttattaaa ttcacctgat gaatatcata agcaaatagc atagaaaata
    16321 atgacaattc ccccgagggg accatttcca aagcactgca ccaccaccag gtgcccggat
    16381 gatcttgctt cagtggtaag agtacctgat tcattgttcc caaaatgatg ttttccccgg
    16441 ccccgccaat aatgtgcagc tctcacgtga taatctattg attaggttat attaagaatc
    16501 aatatttaaa atacatccat tttcttgaat aaatcaacat gtttatttat caattgctgt
    16561 tttagctctg actcatcaag ctctccatat ttgtcctaca gataattgcg aataaataca
    16621 gagaacgcca tttatccttt tagcatacct gccggatatt tttatttgta ttaatattaa
    16681 ttgcatctcc aactccgagt atatccatca ttaaaaactt tttgagatta tagaatacct
    16741 gtgataacaa gtgaaacatc tgcacaatgt gaatagttag tttcttttca gaggaaatga
    16801 aatagacaag ctgaatgaag gggagagtga tataaatcaa aaaacataac atgaatttaa
    16861 ttaattacac ccatcattgt ggataaagtt actgaataat aaataacata atcttctggt
    16921 atcccactca aataatttca ttccttttta atacttcttg taatttcggt aactgacata
    16981 cagcatcagc gatcgcgctg atattctgcg cattatcctg aaaccattca tgccccggcc
    17041 cccatgtgcg catggtgcag agataacagt ccaccagcgt tagttgctca ccaaaggcat
    17101 aaggttcagc gg
//
LOCUS       DACAPB010000091        16905 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.091, whole genome shotgun sequence.
ACCESSION   DACAPB010000091 DACAPB010000000
VERSION     DACAPB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.091"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..345
                     /locus_tag="IT904_002681"
     CDS             <1..345
                     /locus_tag="IT904_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAP3192445.1"
                     /translation="QITKPRLLEQQQLREQRLQRQRQRQFTRDWQSALEALAALLPEH
                     AWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQGRWQFEY
                     QLTRKVSDEHVL"
     gene            329..769
                     /gene="hofO"
                     /locus_tag="IT904_002682"
     CDS             329..769
                     /gene="hofO"
                     /locus_tag="IT904_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAP3192446.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            759..1163
                     /gene="hofP"
                     /locus_tag="IT904_002683"
     CDS             759..1163
                     /gene="hofP"
                     /locus_tag="IT904_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAP3192447.1"
                     /translation="MRVKRWLLAGVALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTSGKAAKSDADGG"
     gene            1075..2313
                     /gene="hofQ"
                     /locus_tag="IT904_002684"
     CDS             1075..2313
                     /gene="hofQ"
                     /locus_tag="IT904_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAP3192448.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPLFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            2714..3235
                     /gene="aroK"
                     /locus_tag="IT904_002685"
     CDS             2714..3235
                     /gene="aroK"
                     /locus_tag="IT904_002685"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAP3192449.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            3292..4380
                     /gene="aroB"
                     /locus_tag="IT904_002686"
     CDS             3292..4380
                     /gene="aroB"
                     /locus_tag="IT904_002686"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP3192450.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            4472..5770
                     /gene="damX"
                     /locus_tag="IT904_002687"
     CDS             4472..5770
                     /gene="damX"
                     /locus_tag="IT904_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAP3192451.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAATPAPKETATTAPVQTASPAQTTATPAAG
                     GKTAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVL
                     VSGVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            5877..6713
                     /gene="dam"
                     /locus_tag="IT904_002688"
     CDS             5877..6713
                     /gene="dam"
                     /locus_tag="IT904_002688"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP3192452.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            6731..7408
                     /gene="rpe"
                     /locus_tag="IT904_002689"
     CDS             6731..7408
                     /gene="rpe"
                     /locus_tag="IT904_002689"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP3192453.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            7401..8159
                     /gene="gph"
                     /locus_tag="IT904_002690"
     CDS             7401..8159
                     /gene="gph"
                     /locus_tag="IT904_002690"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAP3192454.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            8152..9156
                     /gene="trpS"
                     /locus_tag="IT904_002691"
     CDS             8152..9156
                     /gene="trpS"
                     /locus_tag="IT904_002691"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP3192455.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            9446..10351
                     /locus_tag="IT904_002692"
     CDS             9446..10351
                     /locus_tag="IT904_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192456.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            10368..10730
                     /locus_tag="IT904_002693"
     CDS             10368..10730
                     /locus_tag="IT904_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP3192457.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            10814..11895
                     /locus_tag="IT904_002694"
                     /pseudo
     CDS             10814..11895
                     /locus_tag="IT904_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            11895..13121
                     /locus_tag="IT904_002695"
     CDS             11895..13121
                     /locus_tag="IT904_002695"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP3192458.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            13118..13996
                     /locus_tag="IT904_002696"
     CDS             13118..13996
                     /locus_tag="IT904_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAP3192459.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGISVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            14007..14360
                     /locus_tag="IT904_002697"
     CDS             14007..14360
                     /locus_tag="IT904_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAP3192460.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            14372..15676
                     /locus_tag="IT904_002698"
     CDS             14372..15676
                     /locus_tag="IT904_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192461.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            15688..16773
                     /locus_tag="IT904_002699"
     CDS             15688..16773
                     /locus_tag="IT904_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP3192462.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
BASE COUNT         3999 a         4099 c         4819 g         3988 t
ORIGIN      
        1 cagataacga aaccacgttt gctggagcaa cagcagttac gcgaacagcg tttgcaacgg
       61 cagcgccagc gacaatttac ccgcgactgg caatctgcgc tggaagctct ggcggcgctt
      121 ttacccgagc acgcctggct gacaacgata agctggcagc agggaacgct ggagatcaag
      181 gggcttacaa caagcattac cgcgttaaac gcactggaaa cgtcactccg ccaggatgct
      241 tcttttcatc tcaatcagcg gggggccacg cagcaggatg cgcagggacg ctggcaattt
      301 gagtatcagt taacaaggaa ggttagcgat gaacatgttc tttgactggt ggttcgccac
      361 atcaccccgt ctccgccagc tttgctgggc agtctggttg ctgatgttag ttacgctcat
      421 ttttctgtca tcgacacacc aggaagagag cgacgcatta attcgactac gggcaagtca
      481 tcaccagcag tgggccgcac tgtatcgcct ggtagacacc actcccttca gcgaggaaaa
      541 aacgctgccc ttttcgccac tggattttca gttatccggc gcgcaactgg tttcctggca
      601 tccatccgcg cagggaggcg agttggcgtt gaaaacgctg tgggaagcag tgccgtcggc
      661 atttacacgg ctggcagagc gcaacgtcag cgtgagccgt ttttcgttaa gcgtggaagg
      721 tgatgatctt ttgttcacgc tacaactgga gacgccgcat gagggttaaa cgctggttgt
      781 tggcaggtgt tgcattgtgc cttttaaccg gtatgcgtga cccttttaaa ccgccggaag
      841 atctatgccg gattagcgaa cttagccagt ggcgctatca ggggatggta gggcgaggcg
      901 agcacatcat cggtgtaata aaagacgggc aaaagaaatg gcgacgggtg cagcaaaacg
      961 atgtgctgga aaacggctgg acaattttac agctgacgcc agacgcacta acgctgggaa
     1021 ccgggacaaa ctgcgaaccg ccacaatggt tgtggcaacg gcaaggagat acaaatgaag
     1081 caatggatag ccgcactact gttgatgctg atacccggcg tacaagcggc aaagccgcaa
     1141 aaagtgacgc tgatggtgga tgacgttccg gtagctcagg tgttgcaggc gctggctgaa
     1201 caggagaagt tgaacctggt cgtgtcgcca gacgtcagcg gtacggtgtc gttacatcta
     1261 acagatgttc cctggaagca ggcactacaa actgtagtga aaagcgccgg actgataacg
     1321 cgccaggagg gcaacattct ctcagtgcat tccattgcct ggcagaataa caatatcgcc
     1381 cgccaggagg cggagcaggc gcgggcgcag gcaaatctgc cgctggaaaa tcgcagtata
     1441 accctgcaat acgccgacgc gggagaactg gcgaaagcgg gggagaagct actgagtgcc
     1501 aaagggagta tgaccgtcga taaacgcacc aatcgccttt tgctacgaga taacaaaacg
     1561 gcgttaagcg cacttgaaca gtgggtagcg caaatggatc tgccggtcgg gcaggttgag
     1621 ctgtcggcgc atattgtcac cattaatgaa aaaagtttgc gtgagttagg cgtgaaatgg
     1681 acgctggccg atgcgcaaca cgctggtggc gttgggcaag tcaccacgct tggtagcgac
     1741 ctctccgtag cgacggcgac aacgcatgtc ggttttaaca ttgggcgcat caacggacgt
     1801 ttgctggatc ttgagctttc tgcgctcgaa caaaaacagc agctggatat tatcgctagt
     1861 ccgcgtctgc tggcctcaca tcttcagcct gccagcatta aacaggggag cgaaattcca
     1921 tatcaggttt ccagcgggga aagtggcgcg acgtcggtgg aatttaaaga ggccgtcctg
     1981 gggatggagg tcacgcccac ggtgttacaa aaaggtcgca tccggctgaa attacacatc
     2041 agccagaacg ttccggggca ggtgctacag caggctgatg gcgaagtgct ggcgattgat
     2101 aagcaggaga tcgaaacgca ggtcgaggtc aaaagcggag aaacgttggc gctgggcggc
     2161 atttttaccc gtaaaaataa atcgggtcag gatagcgtac cgttgcttgg cgacattccc
     2221 ttgttcgggc aattatttcg tcatgacgga aaagaagatg aacgacgcga gttagtggtg
     2281 tttatcacgc cacgactggt ttccagtgag taaacagccg taaaagcggt aatgttttta
     2341 cgctgaacgt gtttcatcta tttgacgcgc gcaggtattt agcatacaag gagtaccgat
     2401 ttgagagttg gtgctcttcg ctgcctgcgt tccatgatga tgatttatca ttcaggcggc
     2461 attttgctgt cttttttacg ctaatcttac ccggtgattt atcgccagag cggtggtagc
     2521 aaggcagcgc gcttgcagcg accagatatg cagagggatg ggtgatttat tcagttgcca
     2581 aacccgctgg agtattgaga taattttcag tctgactctc gcaatatctt atgaggtttc
     2641 agttcatgtc ctgcggcgct ctctgagcga agcgggttta tcattaacga atagtcttag
     2701 tagtaccgaa aaaatggcag agaaacgcaa tatctttctg gttgggccta tgggtgccgg
     2761 aaaaagcact attgggcgcc agttagctca acaactcaat atggaatttt acgattccga
     2821 tcaagagatt gagaaacgaa ccggagctga tgtgggctgg gttttcgatt tagaaggcga
     2881 agaaggcttc cgcgatcgcg aagaaaaggt catcaatgag ttgaccgaga aacagggtat
     2941 tgtgctggct actggcggcg gctctgtgaa atcccgtgaa acgcgtaacc gtctttccgc
     3001 tcgtggcgtt gtcgtttatc ttgaaacgac catcgaaaag caacttgcac gcacgcagcg
     3061 tgataaaaaa cgcccgttgc tgcacgttga aacaccgcca cgtgaagttc tggaagcgtt
     3121 ggccaatgaa cgcaatccgc tgtatgaaga gattgccgac gtgaccattc gtactgatga
     3181 tcaaagcgct aaagtggtcg ctaaccagat tattcacatg ctggaaagca actaattctg
     3241 gctttatata cactcgtctg cgggtacagt aattaaggtg gatgtcgcgt tatggagagg
     3301 attgtcgtta ctctcgggga acgtagttac ccaattacca tcgcatctgg tttgtttaat
     3361 gaaccagctt cattcttacc gctgaaatcg ggcgagcagg tcatgttggt caccaacgaa
     3421 accctggctc ctctgtatct cgataaggtc cgcggcgtac ttgaacaggc gggtgttaac
     3481 gtcgatagcg ttatcctccc tgacggcgag cagtataaaa gcctggctgt actcgatacc
     3541 gtctttacgg cgttgttaca aaagccgcat ggtcgcgata ctacgctggt ggcgcttggc
     3601 ggcggcgtag tgggcgatct gaccggcttc gcggcggcga gttatcagcg cggtgttcgt
     3661 ttcattcaag tcccgacgac gttactgtcg caggtcgatt cctccgttgg cggcaaaact
     3721 gcggtcaacc atcccctcgg taaaaacatg attggcgcgt tctaccagcc agcttcagtg
     3781 gtggtggatc tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc ggggctggca
     3841 gaagtcatca aatacggcat tattcttgac ggtgcgtttt ttaactggct ggaagagaat
     3901 ctggatgcgt tgttgcgtct ggacggtccg gcaatggcgt actgtattcg ccgttgttgt
     3961 gaactgaagg cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg tgctttactg
     4021 aatctgggac acacctttgg tcatgccatt gaagctgaaa tggggtatgg caattggtta
     4081 catggtgaag cggtcgctgc gggtatggtg atggcggcgc ggacgtcgga acgtctcggg
     4141 cagtttagtt ctgccgaaac gcagcgtatt ataaccctgc tcacgcgggc tgggttaccg
     4201 gtcaatgggc cgcgcgaaat gtccgcgcag gcgtatttac cgcatatgct gcgtgacaag
     4261 aaagtccttg cgggagagat acgcttaatt cttccgttgg caattggtaa gagtgaagtt
     4321 cgcagcggcg tttcgcacga gcttgttctt aacgccattg ccgattgtca atcagcgtaa
     4381 caacaagaaa ggtcaggccg cttatcaagc ggtctattag cttcaggtta attgcaacgt
     4441 ggtaagcatt aaccttttag tggggtgtta aatggatgaa ttcaaaccag aagacgagct
     4501 gaaacccgat cccagcgatc gtcgtactgg tcgttctcgt caatcttctg aacgttctga
     4561 gcgtactgaa cgtggcgaac cgcagatcaa ttttgatgat attgaacttg atgacactga
     4621 cgatcgccgt ccgactcgtg cgcaaaaaga gcgcaatgag gaaccggaaa tcgaagaaga
     4681 aattgacgaa tccgaagatg aaaccgtgga tgaagagcgc gtagagcgtc gtccgcgtaa
     4741 gcgcaaaaaa gcagccagta aacccgcttc tcgtcagtat atgatgatgg gcgtcggcat
     4801 tctggttcta ctgctgttga tcatcggtat cggttctgcg ctaaaagccc cctcgaccac
     4861 ttccagcgat caaaccgcgt ctggcgagaa gagtattgat cttgctggca atgcgaccga
     4921 tcaggcgaat ggcgtgcagc cagcgccggg aaccacgtct gcggaaaata ctcagcagga
     4981 tgtttctctg ccaccgatct cttctacgcc gactcaaggg caaaccccgg tcgcaacgga
     5041 tggtcaacaa cgtgttgaag tgcagggtga cctgaacaat gcgctgaccc agccacaaaa
     5101 tcagcaacag ttgaacaatg tggcggtcaa ttctacattg ccgactgaac ccgcgactgt
     5161 cgcgccagta cgcaatggca atgcatcgcg tgacacggcg aaaacgcaaa ctgctgaacg
     5221 tccggccact acgcgtccag ctcgtcagca ggcggtgatt gaaccgaaaa aaccgcaagc
     5281 aaccgtgaaa acggaaccga agccggtagc acagacgccg aagcgtactg aaccagctgc
     5341 ccctgtggcg agcacgaagg caccggctgc gacttctacg ccagccgcaa cgccagcacc
     5401 aaaagagacg gcgactacgg ctccagtaca gacggcatcc ccggcgcaaa ccacggcaac
     5461 accagccgct ggagggaaga ccgcaggtaa tgttggttcg ttgaaatcgg caccgtccag
     5521 ccattacact ctgcagctga gcagttcctc taactacgac aacctgaacg gttgggcgaa
     5581 gaaagagaat ctgaaaaact acgttgtcta tgaaacgacg cgtaatggtc agccgtggta
     5641 tgtcctggtt tctggcgtgt acgcttcgaa agaagaggcg aaaaaagcgg tatctacatt
     5701 gccagcagat gttcaggcca aaaacccgtg ggcgaaaccg ctgcgtcagg tacaggccga
     5761 tctgaagtaa tcaaggttat ctcccgcaat ggtttatcgt tgcgggagtt gcctgaagcg
     5821 ctggatgctg tcggagcttt ctccacagcc ggagaaggtg taattagtta gtcagcatga
     5881 agaaaaatcg cgcttttttg aagtgggcag ggggcaagta tcccctgctt gatgatatta
     5941 aacggcattt gcccaagggc gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt
     6001 ttctcaacac cgacttttct cgttatatcc ttgccgatat caatagcgac ctgatcagtc
     6061 tctataacat tgtgaagatg cgtactgatg agtacgtaca ggccgctcgc gagctgtttg
     6121 ttcccgaaac aaattgcgcc gaggtttact atcagttccg cgaagagttc aacaaaagcc
     6181 aggatccgtt ccgtcgggcg gtactgtttt tatatttgaa ccgctacggt tacaacggcc
     6241 tgtgtcgtta caatctgcgc ggtgagttta acgtgccgtt cggccgctac aaaaaaccct
     6301 atttcccgga agcagagttg tatcacttcg ctgaaaaagc gcagaatgcc tttttctatt
     6361 gtgagtctta cgccgatagc atggcgcgcg cagatgatgc atccgtcgtc tattgcgatc
     6421 cgccttatgc accgctgtct gcgaccgcca actttacggc gtatcacaca aacagtttta
     6481 cgcttgaaca acaagcgcat ctggcggaga tcgccgaagg tctggttgag cgccatattc
     6541 ctgtgctgat ctccaatcac gatacgatgt taacgcgtga gtggtatcag cgcgcaaaat
     6601 tgcatgtcgt caaagttcga cgcagtataa gcagcaacgg cggcacacgt aaaaaggtgg
     6661 acgaactgct ggctttgtac aaaccaggag tcgtttcacc cgcgaaaaaa taattctcaa
     6721 ggagaagcgg atgaaacagt atttgattgc cccctcaatt ctgtcggctg attttgcccg
     6781 cctgggtgaa gataccgcaa aagccctggc agctggcgct gatgtcgtgc attttgacgt
     6841 catggataac cactatgttc ccaatctgac gattgggcca atggtgctga aatccttgcg
     6901 taactatggc attaccgccc ctatcgacgt acacctgatg gtgaaacctg tcgatcgcat
     6961 tgttcctgat ttcgctgccg ctggtgccag catcattacc tttcatccag aagcctccga
     7021 gcatgttgac cgcacgctgc aactgattaa agaaaatggc tgtaaagcgg gtctggtatt
     7081 taacccggcg acacctctga gctatctgga ttacgtgatg gataagctgg atgtgatcct
     7141 gctgatgtcc gtcaatcctg gtttcggcgg tcagtctttc attcctcaaa cactggataa
     7201 actgcgcgaa gtacgtcgtc gtatcgacga gtctggcttt gacattcgtc tggaagtgga
     7261 cggtggcgtg aaggtgaaca acattggcga aatcgctgcg gcgggcgcgg atatgttcgt
     7321 cgccggttcg gcaatcttcg accagccaga ctataaaaaa gtcattgatg aaatgcgcag
     7381 tgaactggca aaggtaagtc atgagtaagt ttgaagatat tcgcggcgtc gcttttgatc
     7441 tcgacggtac gctggtggac agtgctcccg gtcttgctgc tgcggtagat atggcgctgt
     7501 atgcgttgga gttacccgtc gcaggtgaag aacgcgttat tacctggatt ggtaacggcg
     7561 cagatgttct gatggagcgc gcattgacct gggcgcgtca ggaacgtgcg actctgcgta
     7621 aaacaatggg taaaccgccc gttgatgacg acattccggc agaagaacag gtacgtattc
     7681 tgcgtaaact gttcgatcgc tattatggcg aggttgccga agaggggacg tttttgttcc
     7741 cgcacgttgc cgatacgttg ggcgcgttgc aggctaaagg cctgccgcta ggcctggtca
     7801 ccaacaaacc gacgccgttc gtcgcgccgc tgctcgaagc cttagatatt gccaaatact
     7861 ttagcgtggt tatcggcggc gatgatgtgc aaaacaaaaa acctcatccg gacccgctgt
     7921 tactggtggc tgagcggatg ggaattgccc cacaacagat gctgtttgtc ggcgactcac
     7981 gcaatgatat tcaggcggca aaagcggcag gttgcccatc cgttggctta acctacggat
     8041 ataactacgg cgaggctatc gatctcagcc agcctgatgt aatttatcag tctataaatg
     8101 accttctgcc cgcattaggg cttccgcata gcgaaaatca ggaatcgaaa aatgactaag
     8161 cccatcgttt ttagtggcgc acagccctca ggtgaattga ccattggtaa ctacatgggt
     8221 gcgctgcgtc agtgggtaaa catgcaggat gactaccatt gcatttactg tatcgttgac
     8281 caacacgcga tcaccgtgcg ccaggatgca cagaagctgc gtaaagcgac gctggatacg
     8341 ctggccttgt atctggcatg tggtatcgat cctgagaaaa gcaccatttt tgttcagtcc
     8401 cacgtaccgg aacatgcgca gttaggctgg gcactgaact gctataccta cttcggcgag
     8461 ctgagccgca tgacgcagtt taaagataaa tctgcgcgtt atgccgagaa catcaacgct
     8521 ggtctgtttg actatccggt gctgatggcg gcggacatcc tgctgtatca aactaatctg
     8581 gtaccggtgg gtgaagacca gaaacagcac ctcgaactga gccgcgatat tgcccagcgt
     8641 ttcaacgcgc tgtatggcga gatctttaag gtgccggagc cgtttattcc gaaatctggc
     8701 gcgcgcgtaa tgtcgctgct ggagccgacc aagaagatgt ccaagtctga cgataaccgc
     8761 aataacgtta ttggcctgct ggaagatccg aaatcggtag tgaagaaaat caaacgcgcg
     8821 gtcactgact ccgacgagcc gccggtagtt cgctacgatg tgcagaacaa agcgggcgtt
     8881 tccaacctgc tggatatcct ttctgcggta acgggccaga gcatcccgga actggaaaaa
     8941 cagttcgaag gcaagatgta tggtcatctg aaaggcgaag tggctgatgc cgtttccggt
     9001 atgctgactg aattgcagga acgctatcac cgtttccgca acgatgaagc cttcctgcaa
     9061 caagtgatga aagatggcgc ggaaaaagcc agcgcgcacg cttcccgtac gctaaaagcg
     9121 gtatacgaag cgattggttt tgtggcgaag ccgtaattca ctattgctgg caaattgcgc
     9181 tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaagtaa tgcacatatt
     9241 gcaaggaaat gtaggcctga taagcgtagc gcatcaggca attttgcttt tatcatcact
     9301 ctgaaaccgg ggaaacccgg tttttttatc ctcatttgca ataatccgaa aaaatgtgaa
     9361 gtgcctcgcc gtttccacat ctcgctgttg cgccttatct tcaaattcct gaagatataa
     9421 atattcaatt tactgaaaat ttattatgcg tcgaacgttt atcaaaaaag aaggcgtcgt
     9481 catcacgacg ctggcccgtt atttgttggg tgaaaaatgc ggtaataggt tgaaaaccat
     9541 agatgagctg gcaactgaat gccgttcatc cgttggcctg acgcaggccg cgttgaaaac
     9601 gctggaatca agcggagcga tacggattga acgccgtggg cgtaatggca gttatctggt
     9661 cgagatggat aacaaagcat tactgactca tgtggatatc aacaacgtgg tgtgtgcgat
     9721 gcccttgccc tatacccgtt tgtacgaagg cctggcgagc ggattgaaag ctcagtttga
     9781 tggcattcct ttttactatg cgcatatgcg cggcgcggat attcgcgtgg agtgtctgct
     9841 taatggcgtg tatgacatgg cggtggtttc ccgactggcg gcggaaagtt atctcacgca
     9901 aaaagggtta tgtctcgcgc tggagttggg gccgcacacc tacgttggcg aacaccagtt
     9961 gatttgccgt aaaggcgagt ccgcaaatgt gaagcgcgtg gggctggata accgttcggc
    10021 ggatcagaaa atcatgaccg atgtcttttt tggcgatagt gatgtggaac gagtcgatct
    10081 ctcttatcac gagagtttac aacgcattgt taaaggcgat gttgatgcgg ttatctggaa
    10141 cgtggtggcg gaaaacgaac tgaccatgct gggattagag gcgacgccgc tcacagacga
    10201 tccgcgattt ttacaggcca ccgaagccgt ggtcctgacg cgagtcgatg attacccaat
    10261 gcaacaactg ctgcgtgccg ttgtagataa acacgccctg cttgcccatc aacaacgggt
    10321 ggtgagtggg gaacaggaac cgagttatta aacgaaaggc atctgatatg gaaaccagac
    10381 tcaacctgct ttgcgatgca ggcgttattg ataaggacat ctgcaaaggc atgatgcagg
    10441 tcgtcaacgt actggaaaca gagtgccatc tgccggtgcg cagtgagcaa ggaacgatgg
    10501 cgatgacaca tatggcgagt gcactgatgc gcagtcgccg tggtgaagaa attgagccac
    10561 tggataacga gttgctggca gagctggcgc aatccagcca ctggcaagcc gttgtgcaat
    10621 tgcatcaggt gttgttgaag gaattcgcac tggaagttaa cccgtgtgaa gaaggctatt
    10681 tgctggccaa cctttatgga ttatggatgg ctgctaacga agaggtttga agctcccgac
    10741 aaatccacgt cgcacaacct aaatattcaa aaaaatgaat atttaattca aggaacagaa
    10801 acgaggcaac aagatgtttg tagaagcatt gaaacgccag aacccggcgc tgatttccgc
    10861 cgcactaagc ctgtggcagc agggcaagat cgccccggac agctgggtga tcgacgtgga
    10921 tcagatactg gaaaacggta agcggctgat tgagacggcg cggctttacg gcattgaact
    10981 gtatctgatg accaagcagt ttggtcgtaa tccgtggctg gcggaaaaat tgctggcatt
    11041 aggctacagc ggcattgtgg cggtggatta caaagaggcg cgagtcatgc gccgcgctgg
    11101 tttgcctgtg gcgcatcagg ggcatctggt acaaatccct tgtcatcagg ttgctgacgc
    11161 cgttgaacag ggcaccgacg tcatcaccgt gtttactctc gacaaagcgc gggaagtttc
    11221 tgcggcggcg gtgaaggccg ggcgaatcca gtctgtgctg cttaaatcca gaatctgcca
    11281 gggctgcatt tagccggact tacccatttc ccttgcctgc tttgggatga ggctgtcgga
    11341 aaagttttgc cgacaccgaa tcttcacacg ctgatacagg cacgggatca actggcgaaa
    11401 tctggtattg cacttgagca actgaacgcg ccttcagcga ccagctgcac ttcgctgcca
    11461 ttactggcgc aatacggtgt gactcatgcc gaacccggtc atgcgctgac gggcactatt
    11521 ccggcaaacc agcagggcga tcagcctgaa cgtatcgcga tgctctggtt aagtgaaatc
    11581 tcccatcatt tccgtggcga cagctactgc tacggcggcg gttactatcg tcgtggtcat
    11641 gcgcaacatg cgctggtgtt tacgccagaa aatcaaaaga ttactgaaac caatctcaaa
    11701 actgtggatg acagcagtat cgactacacc ctgccgctgg caggcgagta tccggtgagc
    11761 agtgcagtgg tgctctgttt ccgcacgcag atttttgtca cccgtagcga tgtggtgctg
    11821 gtgtccggta ttcaccgtgg cgaaccgaaa atcgttggtc gttatgacag tcttggaaac
    11881 tctctggggg cgtaatggcg cgatttgtgg tgttagtgat tgatagcttt ggcgtagggg
    11941 caatgaaaga tgtcacgctg gtgcgtccgc aagatgcggg agcgaatacc tgtggtcata
    12001 tcctgagcca gttgccgcat ttgcagctac caacgctgga gaagctgggg ctaatcaacg
    12061 cattgggtta tgcgccaggc gatatgcagc cgtcagattc cgcaacctgg ggcgtggcag
    12121 agctgcaaca tgaaggtggc gataccttta tggggcatca ggaaatttta ggcacgcgcc
    12181 cgttaccgcc gctgcggatg cctttttgcg atgtgattga ccgtgttgag caggcattag
    12241 tatccgccgg ttggcaggtg gagcgccgtg gcgatgaact gcaatttctg tgggtcaatc
    12301 aggcggttgc gattggcgat aatctcgagg cggatttagg ccaggtctat aacattaccg
    12361 ccaatctctc tgtgatctct tttgacgacg caatcaaaat tggtcgtatc gtgcgtgagc
    12421 aggtacaggt cggtcgggtc attacatttg gtggcctgtt aaccgacagt caacgcattc
    12481 tcgatgccgc agaaagcaaa gaagggcgct ttattggtat caatgcgccg cgttctggcg
    12541 cttatgacaa cggtttccag gtcgtgcata tgggctatgg cgtcgatgaa aaagtgcagg
    12601 tgccacaaaa actgtatgaa gcaggcgtgc caaccgtgct ggtgggtaag gtggcagata
    12661 tcgtcaacaa tccttatggc gtgagctggc aaaatctggt ggatagccag cggattatgg
    12721 atatcaccct caacgaattt aacacccatc cgacggcgtt tatttgcacc aacattcagg
    12781 aaaccgacct cgctggtcat gcagaagacg tcgcacgtta tgccgaacgt ttgcaggtcg
    12841 ttgaccgtaa ccttgcccgg cttgttgagg cgatgcagcc agatgattgc ctggtcgtga
    12901 tggcggatca cggcaacgat ccgaccattg gtcacagcca ccatacccgc gaagtggtgc
    12961 cagtgctggt ttatcagcaa gggatgatcg ctacgcagct cggtgtgcgc accacgcttt
    13021 ctgatgtggg ggctaccgtg tgtgaatttt tccgcgcgcc accgccacaa aatggtcgct
    13081 cttttctttc ctccctccgg tttgcaggag acaccctatg agttttgatc cgacgggtta
    13141 caccctcgcc catgagcatc tgcatattga tctctccggc tttaaaaaca acgtggactg
    13201 ccgccttgat cagtatgcgt tcatttgcca ggagatgaac gacctgatga cccggggcgt
    13261 gcgtaatgtg attgagatga ccaaccgtta catggggcgc aatgcgcaat ttatgcttga
    13321 tgtaatgcgc gagacgggga tcagcgtggt ggcctgtacc ggttattacc aggacgcgtt
    13381 tttcccggaa catgtggcga cccgcagcgt gcaggaactg gcgcaggaga tggtcgatga
    13441 aattgaacag ggtatcgatg gcacggagct gaaagccggg atcatcgcgg agatcggcac
    13501 cagcgaagga aagattacgc cgctggaaga gaaggtattt attgccgctg cgctggcgca
    13561 taaccagacc ggacgcccga tctccacgca tacgtcgttc agcacgatgg ggctggagca
    13621 actggcgttg ctacaagccc acggggttga tctttcgcgc gtcaccgttg gtcactgcga
    13681 tctgaaagac aacctcgaca acattttgaa gatgatcgat ctcggcgcgt acgtgcagtt
    13741 cgacaccatt ggtaagaaca gttactaccc ggacgaaaag cgtattgcga tgcttcatgc
    13801 gctacgtgac cgtgggttgc tgaaccgcgt catgctgtcg atggatatta cgcgccgctc
    13861 ccatttaaaa gccaatggtg gttatggcta tgactattta ttgaccacct ttattccgca
    13921 attgcgccag tcaggattca gtcaggccga tgtggatgtg atgttacgtg aaactccctc
    13981 tcaatttttc caataaggac agactcatga aaaagattgg cgttgcaggc ttacagcgtg
    14041 agcagattaa aaaaaccatt gaagcgacgg ctcctggcag ttttgaagtt ttcattcaca
    14101 acgacatgga agcggcaatg aaagtgaaat ccgggcaact ggattactac atcggtgcgt
    14161 gtaataccgg tgcgggcgcg gcattgtcga ttgccatcgc ggtgattggc tataacaaaa
    14221 gttgcaccat tgccaaacca ggcattaaag cgaaagacga gcatatcgcc aaaatgatcg
    14281 ctgaaggaaa agtggcgttt ggcctttccg ttgagcacgt cgaacacgcg attccgatgc
    14341 tgattaacca tctgaaataa aaggcacgac tatggatctg tatattcaga ttatcgtggt
    14401 ggcgtgcctg acgggtatga catcgcttct ggcgcatcgc tcggcggctg tttttcatga
    14461 cggcatccgc ccgatcctgc cgcaactgat tgaaggctat atgaaccgtc gcgaggcggg
    14521 gagtatcgct tttggtctga gcattggttt tgtggcctcg gtggggatct cttttaccct
    14581 gaaaaccggg ctgctaaacg catggttact ctttcttcct accgatatcc tcggcgtact
    14641 ggcgataaac agcctgatgg cgtttggtct tggcgctatc tggggcgtgt tgatccttac
    14701 ttgcctgttg ccagtaaacc agctgctgac cgcgctgccg gtggatgtat taggtagcct
    14761 cggggaatta agctcgccgg tggtttctgc ttttgcactc ttcccgttgg tggcgatttt
    14821 ctaccagttt ggctggaagc aaagtctggt cgccgccgtg gttgttctga tgacccgtgt
    14881 ggtagtcgtg cgctatttcc cacatcttaa ccctgaatcc atcgaaatct ttattggcat
    14941 ggtgatgctg ctgggaatcg cgataactca cgacctgcgt catcgtgatg aaaatgacat
    15001 cgatgccagc gggctttcgg tgtttgaaga gcgcacgtcg cggattatca aaaacttacc
    15061 ctatatcgcc atcgtgggag cattgattgc cgccgttgcc agtatgaaga ttttcgccgg
    15121 cagtgaagtg tcgatcttca ctctggagaa agcgtactcc gcaggcgtaa cgccggaaca
    15181 atcgcaaacg ctgatcaacc aggctgctct ggcggagttt atgcgcggac tgggttttgt
    15241 gccgttgatt gccaccaccg cgttagccac cggcgtatat gcagttgcgg gctttacctt
    15301 tgtttatgcg gtgggctatc tctcgccgaa tccgatggtt gcggcggtat taggcgcagt
    15361 ggttatttcg gcagaagtcc tgttacttcg ttcgatcggc aaatggctgg ggcgctatcc
    15421 gtcggtgcgt aatgcgtcgg ataacatccg taacgccatg aatatgctga tggaagtggc
    15481 gctgctggtc ggttcgattt tcgcagcaat taaaatggcg ggttataccg gattctctat
    15541 cgcggttgcc atttacttcc tcaacgaatc cctgggccgt ccggtacaga aaatggcggc
    15601 accggtcgtg gccgtaatga tcaccggtat tctgctgaat gttctttact ggcttggcct
    15661 gttcgttccg gcttaaggaa ggcacctatg aagacgtttc ctctgcaaag cctgacgatt
    15721 attgaggcgc agcaaaagca gtttgcgctg gtggatagca tttgtcgcca tttccccggc
    15781 agcgagtttc ttactggcgg tgatttaggc ttaacgccag gactgaatca accgcgcgtt
    15841 acccagcgtg tggaacaggt gctggctgat gcatttcacg cacaggctgc ggcgctggtg
    15901 cagggcgcgg ggactggcgc gattcgcgcc gggctggcgg ctttgctcaa accggggcag
    15961 cgtcttctgg tgcatgacgc gcctgtttac ccgacgacac gggttattat tgagcagatg
    16021 gggctgacgc ttattactgt tgatttcaat gacctgtcgg cactgaagca ggtcgtcgac
    16081 gagcaacaac cggatgcggc gctggtgcag catacgcgcc agcagccgca ggacagctac
    16141 gtgctggcag atgtgctggc aacgttgcgc gcggcaggtg ttccagcgtt aaccgatgac
    16201 aactatgcgg tgatgaaggt ggcgcgaatc ggctgtgaat gcggcgcgaa tgtctcgaca
    16261 ttttcctgct tcaagctatt tgggccagag ggtgttggtg cagtggtcgg cgatgctgat
    16321 gttatcaacc gtattcgcgc cacgctttac tcaggcggta gccagatcca gggcgctcag
    16381 gcgctggaag tcttgcgtgg tctggtgttt gcgccagtga tgcacgcggt gcaggcgggg
    16441 gtatctgaac ggttgctggc tttgctcaac ggtggtgcag tgccggaagt gaaaagcgcc
    16501 gtcattgcta atgcgcagtc gaaggtattg attgtggagt ttcatcagcc gattgccgcc
    16561 agggtgctgg aagaagcgca gaaacgcggt gccttacctt acccggtcgg tgcagagtcg
    16621 aaatatgaaa tcccgccact tttttatcga ctttccggga cgtttcgcca ggcgaatcca
    16681 cagctcgaac attgcgcgat tcgcattaac ccgaatcgca gcggtgaaga gacggtgctg
    16741 cggattttgc gtgagagtat tgccagtatt taatgctgga tagcaccgtc aagctaaatt
    16801 ccgtactgaa cggtcccctc gcccctttgg ggagagggtt agggtgaggg gaacgtacgg
    16861 gatgtaaaat tcaacgcgcc agcatcgccg gatgcgatgc tggcg
//
LOCUS       DACAPB010000092        16606 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.092, whole genome shotgun sequence.
ACCESSION   DACAPB010000092 DACAPB010000000
VERSION     DACAPB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.092"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(71..979)
                     /gene="mak"
                     /locus_tag="IT904_002700"
     CDS             complement(71..979)
                     /gene="mak"
                     /locus_tag="IT904_002700"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAP3192463.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            1104..2015
                     /gene="rdgC"
                     /locus_tag="IT904_002701"
     CDS             1104..2015
                     /gene="rdgC"
                     /locus_tag="IT904_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAP3192464.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(2093..2176)
                     /locus_tag="IT904_002702"
     CDS             complement(2093..2176)
                     /locus_tag="IT904_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192465.1"
                     /translation="MTQRSWSKLQRKTHNIAALKIIARRSE"
     gene            complement(2173..2514)
                     /locus_tag="IT904_002703"
                     /pseudo
     CDS             complement(2173..2514)
                     /locus_tag="IT904_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2662..2946)
                     /gene="ppnP"
                     /locus_tag="IT904_002704"
     CDS             complement(2662..2946)
                     /gene="ppnP"
                     /locus_tag="IT904_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAP3192466.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(3018..3695)
                     /locus_tag="IT904_002705"
     CDS             complement(3018..3695)
                     /locus_tag="IT904_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAP3192467.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3953..4144)
                     /gene="yaiA"
                     /locus_tag="IT904_002706"
     CDS             complement(3953..4144)
                     /gene="yaiA"
                     /locus_tag="IT904_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAP3192468.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(4194..4718)
                     /gene="aroL"
                     /locus_tag="IT904_002707"
     CDS             complement(4194..4718)
                     /gene="aroL"
                     /locus_tag="IT904_002707"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAP3192469.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(4895..5359)
                     /locus_tag="IT904_002708"
     CDS             complement(4895..5359)
                     /locus_tag="IT904_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP3192470.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            5479..6288
                     /gene="proC"
                     /locus_tag="IT904_002709"
     CDS             5479..6288
                     /gene="proC"
                     /locus_tag="IT904_002709"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP3192471.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6305..7420)
                     /gene="adrA"
                     /locus_tag="IT904_002710"
                     /gene_synonym="dgcC"
     CDS             complement(6305..7420)
                     /gene="adrA"
                     /locus_tag="IT904_002710"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAP3192472.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7522..7842)
                     /gene="psiF"
                     /locus_tag="IT904_002711"
     CDS             complement(7522..7842)
                     /gene="psiF"
                     /locus_tag="IT904_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAP3192473.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7961..9376)
                     /gene="phoA"
                     /locus_tag="IT904_002712"
     CDS             complement(7961..9376)
                     /gene="phoA"
                     /locus_tag="IT904_002712"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAP3192474.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     SGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNAVTEVNQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9477..9737)
                     /gene="iraP"
                     /locus_tag="IT904_002713"
     CDS             complement(9477..9737)
                     /gene="iraP"
                     /locus_tag="IT904_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAP3192475.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(9918..10121)
                     /locus_tag="IT904_002714"
     CDS             complement(9918..10121)
                     /locus_tag="IT904_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192476.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            10199..11293
                     /locus_tag="IT904_002715"
     CDS             10199..11293
                     /locus_tag="IT904_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAP3192477.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11317..11529)
                     /locus_tag="IT904_002716"
     CDS             complement(11317..11529)
                     /locus_tag="IT904_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HAP3192478.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            11789..12097
                     /locus_tag="IT904_002717"
     CDS             11789..12097
                     /locus_tag="IT904_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAP3192479.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(12156..13250)
                     /gene="yaiW"
                     /locus_tag="IT904_002718"
     CDS             complement(12156..13250)
                     /gene="yaiW"
                     /locus_tag="IT904_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAP3192480.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITSNLTTAWFAKRVDERRARCMKQ"
     gene            complement(13263..14483)
                     /gene="sbmA"
                     /locus_tag="IT904_002719"
     CDS             complement(13263..14483)
                     /gene="sbmA"
                     /locus_tag="IT904_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAP3192481.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            14835..15992
                     /gene="ampH"
                     /locus_tag="IT904_002720"
     CDS             14835..15992
                     /gene="ampH"
                     /locus_tag="IT904_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAP3192482.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYPRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(15993..16606)
                     /gene="iprA"
                     /locus_tag="IT904_002721"
                     /pseudo
     CDS             complement(15993..16606)
                     /gene="iprA"
                     /locus_tag="IT904_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
BASE COUNT         4136 a         4327 c         3964 g         4179 t
ORIGIN      
        1 caccttttgt aggcctgata agacgcggca agcgtcgcat caggcatgat gcgccaattg
       61 cctacgtttt ttactcttgt ggccataacc acgcagcgcc gcgtacgccg ctggaatcac
      121 cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc gaagacaaat tgtttaatca
      181 actgcccaac cgtttgatat aaacggtcta cattgctcat cccgcccccc aggacaatca
      241 catccggatc gagaatattc acgacatgtg ccagcgattt tgccagccgc agctcgtagc
      301 gacgcaatgc cagttccgct accggatcgc tttcttcaac caggcggata atttcactgc
      361 ctttcagcgc atgtccgctc aaacgacgat aatccatcgc gaatcccgtg cccgaaataa
      421 aggtttcaat acaaccttgt ttaccgcaat aacaagggac ttcctcgcga taacgcagtt
      481 cgtcttcgtc catccacggt agcggattgt gtccccactc acctgccgtg ccattgccgc
      541 cgatatgcgc ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg ataatcacgg
      601 caaataccgt ctgcgctccc gctgccgcgc catctactgc ttctgaaacc gccagacagt
      661 tagcgtcatt tgccagccgc acttcccgct gcaacctcgc gcttaagtct ttatcgaatg
      721 gctgaccgtt gagccaggtt gaattggcat tcttcaccac accggtgtaa ggcgaaattg
      781 agccaggaat gcccatacct accgttccgc gctgccccgt cgcctgctcc gccatatcaa
      841 ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc acgcggcgtg ggcagacgat
      901 ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac ttcagttttg gtgccgccta
      961 aatcgatacc tatacgcacg gtactctcct tatttttttc aatatcaata gcgtagagac
     1021 ggacaaccgg attggcaatg caaggccgcc gacaattcgt tatcatgccc gctaaattta
     1081 acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt taccgtctta
     1141 gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg atggcattta
     1201 ccccatgcgg tagccaggac atggcgaaga tgggctgggt tcctccgatg ggatcgcaca
     1261 gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat ctgcgcgcgc aaagaagaaa
     1321 aaatcctccc gtctccggtg attaaacagg cgctggaagc gaaaatcgcc aaactggaag
     1381 cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac gaagtgctgc
     1441 actctctgct gccgcgtgct ttcagccgtt ttagccagac aatgatgtgg atcgacacgg
     1501 ttaacggttt gattatggtg gactgcgcca gtgccaaaaa agcggaagat acgctggcat
     1561 tactgcgtaa aagcctgggg tcgttaccgg ttgtaccatt gagcatggaa aacccgattg
     1621 aactgacgct gaccgaatgg gttcgctccg gtagtgcggc acagggcttc cagctgcttg
     1681 atgaagccga gctgaaatcg ttgctggaag atggcggcgt gatccgcgcg aagaaacaag
     1741 atctgaccag cgaagagatc accaatcaca ttgaagccgg aaaagtggtg actaaactgg
     1801 cgctcgactg gcagcagcgc attcagtttg tgatgtgcga cgatggttcg ctcaagcgtc
     1861 tgaagttctg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa gatttcgccc
     1921 agcgttttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta attcaaaacc
     1981 tgattgaagg attaggtggc gaagcacaac gttaattgct gattttcctt taatgccgga
     2041 tgcgacgcct gccgcgtctt atccggcgta cgaagccaca ccaggcatat aattattcgc
     2101 tacggcgagc aataattttt agcgcagcaa tattatgcgt tttacgctgt aacttgctcc
     2161 atgaacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc gaattttccg
     2221 cgttgccccg gttttcacta aatcacgtaa cagcgaaaga tcgggttgtt cgacaaataa
     2281 gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca gctgtggatt
     2341 attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaaca acgccggggt
     2401 atgtgcgttg cgcaatgccg tttggcgtaa atgatcattg cgactttgca gcaatgcacg
     2461 ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaacaa tatctggaga
     2521 tcttcactga tatttatatc aaccttgata aattatgaat atatcggaaa ttgaaaaaat
     2581 cgcagcaaag gaaatattat tatgggttaa agaaaggata tttcagaaaa atgccctccc
     2641 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg gttcggcaac
     2701 ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacctg
     2761 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
     2821 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacac tggcgcgacc
     2881 agtgctgctg ctggaaaagc cgatcgattt cactttgccg gaaaagtact cattactttg
     2941 aagcataaac tggccctgtc tggtagttga atatctactt cactataggg gccagaggcg
     3001 ctgactgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
     3061 gacagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ttggtgaaat
     3121 cctaaacaat ccagcatgat gacatcagct ccttttgcca gtaattcttt cccggcatca
     3181 atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaataccgg cgatttctgc
     3241 aaaatttgcc atttttgcgc ctgaacgggc agcatctcct caaccggaac gataaccccc
     3301 acttgatgat cttcaacaat agaggaaacc agcggtggca atatccgcga cggctcaaga
     3361 aagatcgtat tacgcgcagt catactacta atgtttgccg tactcattaa tataatgacg
     3421 tcgtaacccc gattatcgag tacttcaacc acaccttgca ggtcacgctc cactttgcga
     3481 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc ttcgcctgct
     3541 tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcag gctatgatgg
     3601 gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg cataggtaca
     3661 atgccgatgg tcaggatcgc cagcgacgca ctcattgttc atccacctta cttttctttc
     3721 gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt gctacaaatg
     3781 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
     3841 atggattgtt attgtttaat attctaatta ttagaaaaac atgaattatg aaaaatgtga
     3901 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggtcactctt
     3961 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagcggtc ggatgttcac
     4021 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
     4081 ctggttttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
     4141 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcga aaatcaacaa
     4201 ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg ttcgtttgtt
     4261 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
     4321 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
     4381 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
     4441 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
     4501 gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
     4561 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
     4621 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
     4681 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
     4741 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat accatcaaac tagtacaatt
     4801 tctattgtaa aggaaaaatt ccacttaaag tgaaaatctc aatacacccc ttactatacc
     4861 aataaatatt caagaatgaa gtgtaaataa taaattacat ttggccgcga ctacgttgca
     4921 cttccagcca ccatttctcc agctccgcag caaaggcctg gcggtcacgt tgtgaaagac
     4981 tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
     5041 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgcggatta atcgccgcag
     5101 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatcgcccg
     5161 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
     5221 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
     5281 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
     5341 catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt gtgtcgcgct
     5401 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
     5461 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
     5521 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
     5581 tataccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
     5641 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt taaacctggc
     5701 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
     5761 tctattgctg caggtgtcac gctcgaccaa cttgcccgcg cgctgggcca tgaccggaaa
     5821 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
     5881 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
     5941 ggcgaagcgg aagtgattgc tgagccgatg atccacccgg tggtcggtgt gagcggttct
     6001 tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
     6061 atgccacgcg cccaggcgta taaatttgcc gctcaggcag taatgggttc cgcaaaaatg
     6121 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
     6181 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
     6241 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
     6301 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg taaagcgcca
     6361 aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc ggcgcaaccc
     6421 ccacactaat ccgtaaagtt acctgtggcg tattcggcaa acgtaatgta tttagccctt
     6481 catgcacccg taacatggcg gtaatagcgc tctcagctgg cgtaccggac atgattactg
     6541 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
     6601 gtcgggtaag cgccactatc gcttcatcgc ccacatcatg gccccaggta tcgttgatgc
     6661 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgt cgccgacagt
     6721 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
     6781 cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
     6841 ggtagctgac ccagccaaac agcagaggat aaatgacaat aatgggaagg gagagccacc
     6901 attccagcgg cgcactattg aacgacacgg taatgcccgt cagctcgagg gtgacaaggc
     6961 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
     7021 aattcagaca cataatcatc aacatcgcgg tggaaggcag cacgtttacg cccattacgc
     7081 ctacccacat tcccgctaat actgcatcgg tttttaaatt gtaaatttcc cggctaagcg
     7141 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
     7201 ccaacaccag ccaccaccag cccggcggcg ggtgtgaaac cagcgttgaa gcaatcggta
     7261 agaacatgcc agccaggcca accgcgcggg gtagtctgac gcgacgggcg aagcgcagcc
     7321 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
     7381 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
     7441 ttatagagac ggagctaaag gcagaagtat gaaatttcgg gtgagccatt ttcgtagctc
     7501 acccgttagc actatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt
     7561 acgatcatcc cctttcagag attgttgcgt tgcttgatta ttacattcgc gcatcttttg
     7621 ctgctgtggc gtcaaacttt tttcgccagg cgcagacttg ctgttcttca ggcaatcact
     7681 catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga
     7741 ggtcatacgc tgttgttgtg gggttaaagt tctctcggca gcgccgacag tggttaaaaa
     7801 aaccagacca aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat
     7861 gttttaacca tgagcgtttg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa
     7921 aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc cccagggcgg
     7981 ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg
     8041 ggccatacgc cgcaatacgc aactgactgc ctgtatgttc ttgtgaatcc tcttcagagt
     8101 tcccgtaact catcaccatc actgcgccat ctttggtatt tagcgcctgg gtgaggcccg
     8161 gagctttggt atccggcgca acaatctggc tggcgtgggc gtgatcagcg gtgactatga
     8221 ccagcgtgtt accctccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat
     8281 cgaccgtctc gccaatttgc ccacaaggat tcgcagcatg atcctgttta tcgattgacg
     8341 caccttcaac ttgcaggaaa aagcctttct catttttact caacaattca atggctttgt
     8401 cggtcatctg cgccagggtt ggtacactgt cattacgttg cggattaggc gtacaggtga
     8461 ctgcgggctt atcgatattg ccatggtacg ttgctttcgg tcctagccag cgcactggca
     8521 tattgccgtc agcaaacagg ccaagcaggg gtttttgctg attcacttcc gtcaccgcat
     8581 ttagcgaggc ggcatcgttc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg
     8641 tttttccctg ccattcaccg gcggttgccg tttcagcaaa ggtttttgcg ccaccgccaa
     8701 gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt
     8761 ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgagg
     8821 tcacatgcgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac
     8881 cggtcgccag acctgaggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa
     8941 tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg gttgctgatg
     9001 cagccgagtc ggtgacgtag tccggtttgc cggttttttt attcagcgca tagtgagtgt
     9061 attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat
     9121 aattacgtgc ggcagtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat
     9181 tttttgcagg tttatcgcta agagaatcac gcagagcggc ggtctgatca cccgttaagc
     9241 ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca
     9301 tttctggtgt ccgggctttt gtcacagggg taaacagtaa cggtaagagt gccagtgcaa
     9361 tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag
     9421 cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac
     9481 tgacgaggat gcttcaataa cttctttacg taatcgcgca gcagctccgt atcgtcgtca
     9541 ggaatgctgg catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaaccgc
     9601 tgttggtcat tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct
     9661 acctgcgcac acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca
     9721 gcaatgagat ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg
     9781 tttcacaact tcatagtgtc tggaagtatt atttttatga caaaggaaat actttgcgca
     9841 aatatttttt gtttgattac cagcgaaacg tttctcttat ttaaataact accacacaaa
     9901 atataggctt ttaattatca acctttatta taaataaaag ttatgaaata tattctcttc
     9961 gttaacttat aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt
    10021 agcttcccgt tcatcatttt tgtgatcttg cgcacatctt tgagataacg cactttcttc
    10081 accagtacgt tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa
    10141 tcccccattt tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat
    10201 ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct
    10261 gcaatcggca aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct
    10321 gggcattgat aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc
    10381 agacgatcct gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg
    10441 taaacatgag catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt
    10501 cattttcccg attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg
    10561 ggtcgccaat ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa
    10621 agatgtcacc aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct
    10681 gacgcgcgct aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc
    10741 actgtttgta aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga
    10801 agaacagtac gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga
    10861 gcaagggatc aaaggtcgtg agatcgaatg cgcagttcta ggcaacgaca atccgcaagc
    10921 cagcacctgt ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat
    10981 tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa
    11041 gatccgggcg attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt
    11101 agacgtgttt ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg
    11161 cttcaccaac atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga
    11221 tctgatcaca cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa
    11281 aaccacaatg taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc
    11341 atcgcgacga cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc
    11401 gacggcatag gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag
    11461 aatgaatata aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg
    11521 cagattcatg gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt
    11581 ctgtggtgta gacaacattt tcgtaattgt taataaattt aacttaaggt tgccattttg
    11641 tgactcacaa cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct
    11701 gatattgaca atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa
    11761 gagaagcaga tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt
    11821 tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt
    11881 gctgttttgc ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt
    11941 ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg
    12001 tgtcggcacc attttcgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtggt
    12061 ggcgctatgg ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatacggcc
    12121 taaaacgcct tatctggcat gacattcctg tttgatcact gtttcataca acgcgcccgc
    12181 cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt tgctggtgat tttcgggctt
    12241 tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca
    12301 agttgataaa ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct
    12361 aactgacggc gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc
    12421 agttcggttt tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc
    12481 ttcacgccgc tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag
    12541 ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt
    12601 aaatggtaag taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta
    12661 ccgtccattt tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc
    12721 atcggcccac cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc
    12781 atgctgatca gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac
    12841 cgttcgctat aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa
    12901 gcaggaatgt gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag
    12961 cccggaacgg ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag
    13021 cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc gagatctttc
    13081 gcccaggcat cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgccaca
    13141 tctatcgctt tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa
    13201 accagaaccg ccaacaatga cagcgaagat agaggattga cgcgcgacat tacttctcct
    13261 ttttagctca aggtatgggt tacttcctga attttgtcac catccagttc atgttcaaag
    13321 ctgcgcagac gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg
    13381 tactggaaag caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc
    13441 gtaatcgtac cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc
    13501 tgcaaataga gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcggaaatag
    13561 tttttccgca cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcacg cgtggcatcg
    13621 tcttcaccat aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc
    13681 agccccggca gtttgatccc taccactgcc agcaatcctg tccccatcag cgaccagacg
    13741 atagcggcaa tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc
    13801 gtggagagcg ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa
    13861 ctgacgccca tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt
    13921 gcggcccctt cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc
    13981 gtacgccagc ggaacacgta gtgactgaca aagaagttgt tcagcacact gatcaccaca
    14041 gcgatcagcg caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact
    14101 ttatgcggcg aacttagcgc ggtttgaatc agatcataga acggtgcata ccaggcgttg
    14161 acggcgaccc cgacttccac caaaaaccag gtgacgaaga tgatcagtgc agtaccgagt
    14221 attgaccagt attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct
    14281 acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg
    14341 ctaatcggga tctgcccgga agcgccagtg atacgcgcca cccagtctcc cccaccggct
    14401 tgccagaaga taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgctcccggc
    14461 tttgggaaaa aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg
    14521 ctgtgtatta accgcatgac cactacaaag cgtcagccaa atgaaaccgc ccattgtgac
    14581 ccatagcaag gtgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca
    14641 aaccaaagat tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg
    14701 tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa
    14761 ttcgcccttt gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatt
    14821 tatggacacc accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat
    14881 tgacctccgt ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg
    14941 atcgttatgc cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta
    15001 tcgacggtaa ccagcgcgtc tttcgtagtt atggcgaaac gcgacctggt aataacgttc
    15061 gcccgcagct ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa
    15121 tgctggtgaa attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg
    15181 ctccgccagg cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg
    15241 caacccatac cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt
    15301 ttgtctggcc aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg
    15361 cgccaggcag tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg
    15421 cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccattgg
    15481 ggatgaaaga caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg
    15541 agcgcggtgc cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt
    15601 ccacgcctgg cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc
    15661 gcagtaacca ggcagatcgc atgcagacgt tgatttatcc gcgcgcgcag tttacgaaag
    15721 tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg
    15781 ccccgaaaga gggtcgtccg gggattattc agaagacagg cggtggtggc ggtttcatta
    15841 cctatatggc gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc
    15901 cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg
    15961 ggaataaacc gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata
    16021 gcgaccagtt tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact
    16081 tctgcgacga cagaacgcga tatgcgtgta cgttgatgga tataattcat cacaccaata
    16141 cgtgatcgca attcttcatt ccagtcaatc attgataata atgttgcgcg gatttgttcg
    16201 taggaattat gcccaatgag ctgcacgtcg cgtaattcca gaattctatt ttgccaggct
    16261 aaccagtaaa aagcgtctca ccagagttga ttttgttcaa taagcgtaat ggtttgtttg
    16321 gctggtagat gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt
    16381 ttcattaaac catcagccag ccccataata taaggagcct gggtaatacc gataagtacg
    16441 ttttcttctc tacgcagaga gataactccc tctaaaataa cgaaagtatc ttcgctattg
    16501 acatcactgg aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg
    16561 gacaaacatt tatcgagctt accaaattcc tgaagaggtt taacta
//
LOCUS       DACAPB010000093        16599 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.093, whole genome shotgun sequence.
ACCESSION   DACAPB010000093 DACAPB010000000
VERSION     DACAPB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.093"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(464..823)
                     /locus_tag="IT904_002722"
     CDS             complement(464..823)
                     /locus_tag="IT904_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192483.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGVEVALEYTAERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            complement(987..1937)
                     /locus_tag="IT904_002723"
     CDS             complement(987..1937)
                     /locus_tag="IT904_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192484.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKSE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNTDIIQVQLSITAADEAPTITRKKSLPGCRDRKSQC"
     gene            complement(1964..2356)
                     /locus_tag="IT904_002724"
     CDS             complement(1964..2356)
                     /locus_tag="IT904_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HAP3192485.1"
                     /translation="MTFTEIRNTVISRMTAQTVIDGKDVLYPNGPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPVVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQVVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            complement(2353..2733)
                     /locus_tag="IT904_002725"
     CDS             complement(2353..2733)
                     /locus_tag="IT904_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP3192486.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAIQILTAIDIAAPVDTG
                     RFRNNNMVSLQYPDFGISDNVDPNGTIAVQRGIGVISKAANYGIIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFNGVLQAYK"
     gene            complement(2734..3117)
                     /locus_tag="IT904_002726"
     CDS             complement(2734..3117)
                     /locus_tag="IT904_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP3192487.1"
                     /translation="MGVREEIQSEVAAAFDEDLADVVSDFSGSYVTYRNWDPVTETGG
                     ESTATYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVGHIIEIYDSVSRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            complement(3117..3512)
                     /locus_tag="IT904_002727"
     CDS             complement(3117..3512)
                     /locus_tag="IT904_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HAP3192488.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFSLKINP
                     SRIPEEVKLAGAYAARIASVGKLFQQKNDSGVIISKAVSADGVSVSKSFAELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            complement(3516..3740)
                     /locus_tag="IT904_002728"
     CDS             complement(3516..3740)
                     /locus_tag="IT904_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192489.1"
                     /translation="MAGLTKEQRAQREAAQKNAVVEQNVEQIQEPQKTQIELVVMVTD
                     YQMFPGAPTIANVHPDEVDNWKALGWKTQE"
     gene            complement(3783..4922)
                     /locus_tag="IT904_002729"
     CDS             complement(3783..4922)
                     /locus_tag="IT904_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HAP3192490.1"
                     /translation="MAGNTLTGLIPTIYTALDVVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTASVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGSFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            complement(5021..5785)
                     /locus_tag="IT904_002730"
     CDS             complement(5021..5785)
                     /locus_tag="IT904_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192491.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLATKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLIGAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLETQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGKIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            complement(5890..7002)
                     /locus_tag="IT904_002731"
     CDS             complement(5890..7002)
                     /locus_tag="IT904_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192492.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKTLNVSDAELTSR
                     LLVALDSLDPSSFTVTRLESLLASVREVNRAAINSMFTSLSGELNELAVYEAGYQLSL
                     FDSLLPDFVADVHPLVGISPDALYAAAMARPFQGRLLSEWASDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLDNKPVGHSVPYLQGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKKIVGETRAKPI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQAFRKAGI"
     gene            complement(6986..8392)
                     /locus_tag="IT904_002732"
     CDS             complement(6986..8392)
                     /locus_tag="IT904_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HAP3192493.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPEKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMKEIRTKADENAAGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIVDGDDFGQKKVEQIRHIMPRCVEIYRRSEGTNGESVW
                     TLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQENSPLYTMANSLEDALDNILQIMAEWIGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            complement(8395..9696)
                     /locus_tag="IT904_002733"
     CDS             complement(8395..9696)
                     /locus_tag="IT904_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP3192494.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLRGQFTNLTSGTVYHQFDRKLNNCEEVEQPGESIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRIIKERFWLYDGNDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPLYAESLEQQVW
                     DEKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            complement(9677..10771)
                     /locus_tag="IT904_002734"
     CDS             complement(9677..10771)
                     /locus_tag="IT904_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP3192495.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     PPKNAQTEMRKTAQKSAQKKSAQTAQKRNGKSQEKKPVSDAYLNEGDAEEISFCPDEF
                     GVSDQQAKFAMLVAQGKKLVEAYRLAGYESEGNAAYVTASQLLRNPKVFRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHHYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGEARQLYAGAK
                     LGKFGVEILLEDKAAARRELLRLLAAGGALCADKRLQELEIERRRMENQKLRKEIETV
                     EDNEHPQPVAININVVDARVRSDEDDLSDA"
     gene            complement(10775..10984)
                     /locus_tag="IT904_002735"
     CDS             complement(10775..10984)
                     /locus_tag="IT904_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192496.1"
                     /translation="MTAQQIADVLDVDLNRLKENREAMTNFYASIRKGRAKGEAELRA
                     ALFKLARKGDAFALRELLRVDKNQD"
     gene            complement(10962..11894)
                     /locus_tag="IT904_002736"
     CDS             complement(10962..11894)
                     /locus_tag="IT904_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192497.1"
                     /translation="MCDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFGDHRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRRGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCILMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            complement(11887..12675)
                     /locus_tag="IT904_002737"
     CDS             complement(11887..12675)
                     /locus_tag="IT904_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAP3192498.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFGPTEIDELLTDVLPGTGNKEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLDPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     AEIQCLFEESALVIIDYQQAIENGFVRMTQRMVAIMHSGEEEYV"
     gene            complement(12812..14269)
                     /locus_tag="IT904_002738"
     CDS             complement(12812..14269)
                     /locus_tag="IT904_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HAP3192499.1"
                     /translation="MNTSHVRVVTHMCGFLVWLYSLSMLPPMVVALFYKEKSLFVFFI
                     TFVMFFCIGGGAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFI
                     DALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGG
                     AKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGI
                     STVSLGGFSTHSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIE
                     LRFFLLIALGVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYANWPTH
                     TIVFLLSSSFFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVT
                     DRVMRSVWSFFFLYTLFTVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFG
                     VLNDIAKYLMCIAMILGRLEIYPVIILFSGFFWRS"
     gene            complement(14466..14651)
                     /gene="rzoD"
                     /locus_tag="IT904_002739"
     CDS             complement(14466..14651)
                     /gene="rzoD"
                     /locus_tag="IT904_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HAP3192500.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(14868..15365)
                     /locus_tag="IT904_002740"
     CDS             complement(14868..15365)
                     /locus_tag="IT904_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAP3192501.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIEVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(15365..15580)
                     /gene="essD"
                     /locus_tag="IT904_002741"
     CDS             complement(15365..15580)
                     /gene="essD"
                     /locus_tag="IT904_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HAP3192502.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(15837..16076)
                     /locus_tag="IT904_002742"
     CDS             complement(15837..16076)
                     /locus_tag="IT904_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192503.1"
                     /translation="MCHPDVVNIAKNDIEINHILSKTWKYILYRLHRAVFFILNPKKR
                     NTDTDNARVAMPCKLAMNMAQKKRGRRINAAGQSP"
     gene            complement(16267..16341)
                     /locus_tag="IT904_002743"
     tRNA            complement(16267..16341)
                     /locus_tag="IT904_002743"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16306..16308),aa:Gly,seq:tcc)
     gene            complement(16345..16420)
                     /locus_tag="IT904_002744"
     tRNA            complement(16345..16420)
                     /locus_tag="IT904_002744"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16385..16387),aa:Thr,seq:tgt)
BASE COUNT         4384 a         4377 c         3409 g         4429 t
ORIGIN      
        1 tcctgacaac aaaaaaccca ccgcgaagtg ggtttcaggc gacataatag tagatatagc
       61 gattacgagg ccacgcaatg cttttctcca ggagcatcat cgatttaatt aaagacacca
      121 tcacatctct gtaacagagt gtacgtaatt aacaactaca cacactgctc ctgaaaatac
      181 tggtcatcca gtgcaaagat cactgcttca aattcatcgc gctcaatcaa taccggatga
      241 gtggctaaat attcatttat ctctgtcaga gataaaggca aaggcacccc agccattcca
      301 gcataacgtc gggcacggga tattaccgaa taggcgtaca acaactcctt aagcaccggg
      361 tctatttctg gttccggtat cggtggcaac ctgagttttt ctcgcttcca tcttgccttt
      421 tccccccttt ctcccccgaa ctccgataac caccgctggg cagctatggc ttttttatcg
      481 tatcctgctt ctgctgctct ttaccctggg cgatgctggc tgcttctgca aggatctgcc
      541 agtacaactc tggattctgc ttaagcagcg cgatccctcg ttctgccgta tattccagtg
      601 caacctcaac accattaacc agttcaccaa cccctttcca gtctttcagc agataacgag
      661 cggcattatc aatgagtaaa tcatcaacag aatccacctc ggaaaccttt gaaatatcaa
      721 actccttcgt tccgacgtgc aaactggcat ccattttctc aatgtggcga cggattaatg
      781 cattacggga gcgatactga tcgttatcgc tgcttgccac caacagtttt aacccgtcta
      841 caggttttaa gtccttcatt ggcgtaaacc agcgttctcc accaatgata attttctgat
      901 taagaataaa catccataac ctcattcaat gcgtccccct gtagggcagt accacaggag
      961 gaataacgga aaatcaacta atcgcctcag cactggcttt tgcgatcacg gcagccgggg
     1021 aggctttttt ttctggttat cgtaggtgct tcatctgctg cggtaatgct caattgaacc
     1081 tggataatgt cggtattacc cccatcaggc cattcacctg acacctgaac cttcgggaaa
     1141 ctgaaagtgt atgccccctc tccattcgaa agcgtgaagc tgaacggaac tgtttctcca
     1201 gtcagtgttt tactccagat ttcccacgcg gctttagacc atgaaagcgt caccgtaccg
     1261 gacggtgtaa aagtagtcgg aatatttgct cctgcataag gcgagccagt cccgatacaa
     1321 cgctgtgtct ggagtttgtt atcgaactga atatcaaaac tgtcgataca aaaaccgtta
     1381 cctccggcaa caccattcag gcttactgct gaaacctcct taaacgaata acgtagcttt
     1441 cctgcactat ccacaggttc gcctttgata aaattcgtat catcggcctt tgattcccag
     1501 tccagtccgg caaaggttac ggtcgctgta atatcgccgt cgttagggat ctgcattttc
     1561 catgagccaa cctgcgctcc tctgacgaca gaggcaattc cgacatcgga cgcataggtc
     1621 gccagagaaa atgttattcg ctcattcccc attgtcagag aatcgcctga ccattccgcg
     1681 ccgaaacagg atgcaagaaa atcatcatgt tgaccccagc gaaatttggt accaacatca
     1741 ccgccgacat ccacagtgcc aggcgtcgcc ccctgagcca tccgggagcc accaatctca
     1801 ttattttcgc ttttattctg ggtgggtttt actccccagc ttgtgcgttt taataaactc
     1861 cagtcaccac ttgctggcgt agtgcctgca accgtctccc ggataaatgc cgagataacc
     1921 tttgcacctg aactcacagg agcctcctat gtcattaatt gcgctagagc gcgcgatatg
     1981 gaatttgaag attaagctgg aaccagccat tcttttcgcc aacggctatt gaggaaacaa
     2041 cctggtaact gagacgatcg tcatcctgaa actcaaacag ttcccgcaat ttatcggctg
     2101 tctcagtaat gagttttgaa ccagaacctg cggggacaaa taactgaata atgattatcc
     2161 ccgtgcgata aacaaccggc cccgcgccaa tttcattaac tccagcctgc ccgggaatat
     2221 tacttaaacg cgcccagatt aacttaccgg aaggatcgaa cgttggcccg ttcggataca
     2281 atacgtcttt tccatcaata accgtctgtg ccgtcattct ggaaatgacc gtatttctga
     2341 tttcagtaaa cgtcatttgt aagcctgtaa aacaccatta aaagcgttgg catacacgcc
     2401 agttggcgct tgttgtgaat gaccgttttc aagagcctct gcataaggaa ggttattctg
     2461 gatataaata attccgtaat ttgcagcttt cgaaataact ccgatcccac gctgaaccgc
     2521 aatcgtaccg ttcggatcca cgttatcaga tataccaaaa tcgggatact gtaacgacac
     2581 catgttgtta tttctgaaac gcccggtatc aaccggagca gcaatatcaa tagcagtaag
     2641 aatctgaata gcgatgtagc gaattttcag ccccacatct tcctcaatca tcccggcaaa
     2701 tattgacggt tcgatatccc atgcctttgc catttacgct ctccttaact ggattgagta
     2761 aactgatgcg gaaggatcta cactcgctgt aattacctcg tatcgttgca actgccttga
     2821 tacagaatca taaatctcaa taatatgacc gacagcgggt ttatccgtaa cctcgcatac
     2881 cagagcagtt aatttaaggt caccatgcaa aatattaatc ccatcaattc tgcccagctt
     2941 ataacgcgtc aacacgcctc gcccggtata ggttgctgtg gattcaccgc cagtttcagt
     3001 cacaggatcc cagttccggt acgtaacgta agaaccagaa aaatcactca cgacgtctgc
     3061 taaatcctca tcaaaagcag cggcaacctc tgactgaatc tcttcacgaa cccccatcat
     3121 ccccccctca caaccctgac ttgtgagcga ctaagtccgt acggtttcag cagtgctatc
     3181 gcaagctgta aatcgggttc aagcaatgca gtgctgtttg ctggcaattc agcaaatgat
     3241 ttcgatacac tgaccccgtc agccgacacg gctttactga taataacgcc tgaatcattt
     3301 ttctgctgaa acaacttacc gacagaggca attctggctg catatgctcc cgcaagtttt
     3361 acctcttccg gaatacggga tgggttaatt ttcaggctga agccattaag ccaggcatta
     3421 gccattaaaa cagctttatt tttagcgttc tcactcgtcc aggcgttccc aaacgcatta
     3481 tcaacgtcat cacaggtcac gtaagtgatc atgtgttact cctgagtttt ccagcccaga
     3541 gccttccagt tgtcaacttc atcaggatga acattagcga tagttggtgc gccgggaaac
     3601 atctgataat cggtcaccat aaccactaac tcaatttgcg ttttttgtgg ctcctgaatt
     3661 tgctccacat tttgctccac cactgcattt ttttgcgcgg cttcacgctg tgctctttgc
     3721 tctttagtta atccggccat ataccctcca ttaaaaatgg ggccgaagcc ccatttggat
     3781 gtttaaccaa gaatcagaca accatgtgcc ggtttcactg acgaaacacc ccatgccagt
     3841 ccaacttcat agcgcacctg gcgatactga cgatacagtg caatctggaa cgtaatccct
     3901 gaaatcgggt ccgtaacatt cattacatca tcagcattat cgccaccttc cggcattgcc
     3961 ggagtacggg atgccagcag aaacgcgttg cgatcaaacg ccatatttgc agtaaaagag
     4021 ccaacaactg tgattgcagt atcatcggcc agatcctgac gcagtcccgg cgctgcaaga
     4081 gtaatcagat tgctggtcgc tgctgccaca acatactgat tcggatcgcc agcgaacgta
     4141 acaatctgac ctgcagaaat actccccgaa ccagtatcaa tggaaataag aacatcgcct
     4201 tcttttttct cgccattcac gagataacct gttgcagcaa cctttggcgc tcgttttaca
     4261 cctgccgaac tgtgaatatt gaatccctcc agacgcccca acacgccctc acgcagtagc
     4321 tgttcagtgc cggattcatt cactttaaac aatacagact gttttccgcg caaattagca
     4381 atggcagtgg aaccaagcac catctgcaga tctgttgtcg gtgcgccgtt atcctccaga
     4441 acctgacgag ccagagcagc atcagaaaga tcatctttaa caccaaacgg cgttgtccct
     4501 gcggttccca cggcgcggga agcaccgaaa tacaacgcac caagatcagc ctcaacctcg
     4561 tttgcaaggg cgcgaaaagc ctgcttgaac tgatcagcca gaatggtgtt gtaagtccct
     4621 gaagggccaa gagccagttg ttcttcacca ttccatttaa ccggcgccat tttggattta
     4681 gtaattttta catcaacagt gccaatattt tgatcaccgg tattcggagc tgacggcccc
     4741 ggtacgatat cttcggtttt cgcctcaggc gcaactgacg cggttaccgt ctgatctttt
     4801 gctgcggcgt cagcttttgc gttttttgct accgcaggga taaaacctac ctgctcacgg
     4861 gatacaacat ccagggcggt gtaaatagtc gggatcaacc cggtcagggt atttccagcc
     4921 ataattaaat attccttaaa aatttgcgta attgtgaata gatggagtaa tgagctatcc
     4981 agccctgaca ccagctccca tccggaagct gacaaatgtg ttaatcaaca attgtgatac
     5041 cgtctttcaa tgcgttttgc ttacctgcaa catccagtgc atcaaaagca gagcgtttca
     5101 tcgttttctg accaatatca tgctgtgtcg gacgggagcc gccgccattg ttgccactgg
     5161 ctttcaggat gtagtctttc tgagggtaat tttcgacgag gaactccagc gcctcatcaa
     5221 actgcgccag ttcgccaggc ttcgcgcggg aataaatttt gttgccggaa gcgtcataag
     5281 caacgatctt cccttcttcc actttgaatg cctgcccgaa gcgggcttgt aataaatctg
     5341 ccgggatcgc aattttatcg gcaatatatt ttgaacccgc aaaactaccg ccaatcatag
     5401 aatcgtaaag ctgcgtctcc agcatctgag agcgttgctt ttcttcatct aattgctgct
     5461 gaaaattttt cgtaatttct gccttaacct ggtcaacctg tcccgcaccg atcagctttt
     5521 tctggtcgat ttttgacagc atttccagtg cctcaatcgc cttcttcggg tcttcgatag
     5581 cggcaaactt agccagtttt tcctctgcag cttctttagc caggcgatga ttttttgcct
     5641 cgccattaag ctctgtaatt ttttttgtcg ccagcggtgc atcgaagccg atttctttac
     5701 catcgtcgtg cacatagact ggcaggccag cagtatcaat ttctgcgtat tgttttccgt
     5761 taatctcgac cgttttcagt ttcatattag tacctggttt aagtcttccg acagttgcgc
     5821 tgctcaccat ccggatcgca gcaataaaaa aggccacccg aaggtagcct gttgtaataa
     5881 aggatttatt taaatccctg cttttctgaa tgcctgtgca tcacgctctc ggagttgctt
     5941 cagtgtcagc cattcgcctt tatcggtgta aaattcatct ggcgacatac cgccatcccg
     6001 aatcggcttt gcccgggttt cccccacaat ctttttttgt ctggtgtaag gctggcgcaa
     6061 aaaccattcc ctgtaggttg tatctccggc caccacgcca tccatgctgg cccgctcagc
     6121 cggggaaata tcacgaacat caatacccag ttccttcgct gatttcagaa tgaacgtttc
     6181 cgttgagcga cagcagaaat gaattttccc cggcccctgt aaataaggca cgctgtgacc
     6241 tacaggttta ttatccagcg tatatttgag gcgatccctg attcgacatt gtggcgtagt
     6301 acgattatca agcgttgata accattgctt acccttaatc aaatcattat tcgcgctggc
     6361 aaaactctca cgggcagtag cagcaagatg tccaaccgct gtttttgcaa tgctggccgc
     6421 attagcccga ctcatctgca atgcaccatc ctgaaatccc ttactgacat gtccccgaat
     6481 ttttcttgcg atctgctcat tggtatcccc caacaaaaaa ccctgacgca ccgtatttgt
     6541 tatgcgtctg agccgatccg cctcaagatc tgaggcccac tcactgagca gtcgtccctg
     6601 gaatggtcgc gccattgcag cagcataaag tgcatcaggt gagataccaa ccagaggatg
     6661 aacatcagca acaaaatcag gtagcagaga atcaaacaga cttaactgat aaccagcctc
     6721 ataaactgcc agctcgttca gctctccgga gagactggta aacatgctgt taatagcagc
     6781 gcggttaacc tctctgacac tcgccagaag tgactccagg cgcgtaacgg tgaaactact
     6841 tggatcgagg ctgtccagtg ccaccaacag gcgagatgta agctccgcat cgctgacatt
     6901 cagtgttttc accattctgg cagccacacc agtgctatag cgagatatcc agactgcatg
     6961 agcaattgat tcatcacgca gccgttcatt cacggtttgc atcattgatt tccatcagca
     7021 ttacactctg attttttaat tcatcgatca cttcctctgg acgggaatcc tgatcgataa
     7081 atttcaacgc ctgcaacacc cgaaccgcat caatctgacg tatatcaccg ccctgacgca
     7141 atgactgaac agccagcgcg gaggatgagt caaacacctg ggcagataca tccagttcag
     7201 tgcgcacatc cacattgccg ccgcaactct ctccgatcca ttccgccatt atctggagaa
     7261 tattatcgag ggcatcttcg agggagttcg ccattgtata aagcggcgag ttttcctgca
     7321 tccgctcttc attagtctga tcaacagatt tggtggatgt attttcagcc cgcagaagtt
     7381 tagcgccggc atgacgcatt tgattttcca gcttctccag tgatgtttcg ccagattcta
     7441 tcgctgcccc actatgttca acatattcga ggccattttt tgttctgtcc tcaaaaatcg
     7501 tagcggtgga tgcaccaacc gtcagttctt cattcctgtc cagcccgtag gccaccagca
     7561 atgggacgcg ggcaacatga agaatattgt cctgctcgct ctggctttgc cagtgcttga
     7621 tattcagcaa gccaagatta agcaatggcg gtgtaccacg cataaaccct gttttcttcg
     7681 tatacagtgt taccagagga atatcatcac ggctggtatt ccatgactcg tgaagcgtcc
     7741 agacagattc gccattagta ccttcgctgc gtcgataaat ttcaacgcaa cggggcataa
     7801 tatggcggat ctgctccacc ttcttctgcc cgaaatcatc accatcaaca atgatgacct
     7861 cttttatacg caaatcagta agaacaactt tccctttttc aactttcgat ttccatccaa
     7921 taacctggcg tggattcagc atcgtaacgt atgggcgacc accagccgca ttttcatcgg
     7981 cttttgtccg gatctccttc atatccgttc gtggatagtc caccagcgca tgtgccacac
     8041 catactgaaa tgcgaggctg aaaaattgct gcgcccacac atccagtcgg ctcccctcca
     8101 tgtcgatatt ttctgcatat tccctgattt tttccggcgt ttcctcactc aatactgtcg
     8161 gctctgcaaa tatgcgtcca atattttgct taatgctttc ttcatacaca ggaagtagcg
     8221 tagccacaga caggcgtttt ttataagcgt cttcatcttc gttaggccat tttgggagat
     8281 aattttcccc ctgcctgcgc atttcaagcg taccgcccat cagtgcgtcg ttaatatccc
     8341 acgcctccag catatcgtta tagtcgaggt tgggtgttga tatatcagcc ataattaaat
     8401 ccgaagtgat gtgactcttc cggtcggttt gacaataggg aattgcttaa cgatgaaata
     8461 acctccggca tcattcgggt gatcattgcc agatttttta tcaggctccc ccttctcatc
     8521 ccagacctgt tgctccagag attcggcata taacgggcaa cgcttcacat taactttata
     8581 gcgacgctca ccattggcat tgcagaacat tgcattcatt gagttaacgc gatcttttac
     8641 tggcgggttc gagctgttca ccacaacgtt aaaaccagcc tgcttaagct gggctatatc
     8701 cgtcgtactt gcgttacttg attttctgga atccccggaa gcatctggat aaatataaat
     8761 ttccctcact ttccggtaat cattcccatc atacagccag aagcgttctt taatgatgcg
     8821 gatcatatcc ggcgtatcgt aggcattgat gatttcagtt accgcacatg gaagccccaa
     8881 acgcagcaca tggacgattc ccgccatctt tccaacgtta aaatccatcc caatataaat
     8941 cgactcccct ggctgctcca cttcttcgca attattcagt ttccggtcaa actgatggta
     9001 aacagtacca cttgtcaggt tagtaaactg tcctcgcaga taggctttaa tcagctctgg
     9061 agggtatgat tcaagaagcg aaggaatgta atctgctggc aggttctttt cattatcgaa
     9121 agtagatgcc tgcaccagac catacagtga ggccagctct gttttttcac gcacggcttt
     9181 aacaaactgc tcgtagacaa atttgaatcc ttccggcgtg gttgtaacgt caataccgtt
     9241 gcgaagtcca tcaatcttat aacgcatacg cgcaattatc ttgcgccacg ccgttctggc
     9301 tttttccttc ggcaaaatgt ccagttcatc caccaacgca ttaccaattt tgaaaccgac
     9361 gatcgtttgt ggcttctcca tcgatctgca gatagtggtt ccccgatact ggcgtccgta
     9421 ataaaagtga acctctttat ttccctcatt aatttttacg ttcaatcccc agtcagcagc
     9481 aacttcttcc actgtagggt aaaaaatatc gcgaatttgg ggatacgttg gcgcaaaata
     9541 tccctgattt atacctggat gctcccaaat ccccttgcat atgccgccac acccaaccca
     9601 tgttttgccc gagccaaaac cagcaatata ggctttaaat ttatggggca ttgaaagaaa
     9661 tcgcgcctga ggcacattaa gcgtcggaga gatcatcttc atcactcctt actctggcat
     9721 caaccacatt aatattgatc gccacaggct ggggatgttc attatcttcc accgtttcga
     9781 tctctttgcg cagcttctgg ttttccattc tgcgccgttc aatttccagt tcctgtagcc
     9841 gcttatctgc acataaagcc ccaccagcag caagcaaacg caacaattca cgccgggcgg
     9901 cagccttatc ctccagcagg atctcaacac caaattttcc gagctttgcc cctgcatata
     9961 attgccgcgc ttccccatca agcagagtgg tatcagccat ataaagctgc cccgttccct
    10021 caccgcagca cttcgggcag tccggattgg gtatggcgtt atcaacaaag ccaaggccac
    10081 catattccgg ctcgggtttg ccatctctgg aggcctgcgc cgctgcctta tcgaattctg
    10141 ctatatcgcg ccactggtag tgatgattct cgccccagca ataacggcag ttaacacggc
    10201 gaaattgtgc caactgattg gggtcggcct ggacaatggc catcaactga ctcaccagta
    10261 aatccaggtc tgcggtatag cgtttctggt actgattgcg gaagtagctg atagcacgaa
    10321 aaaccttagg atttcttagg agctggctgg cggttacgta ggccgcattc ccctctgact
    10381 cgtatcctgc caggcgatat gcctcaacga gttttttccc ctgggcaacc agcattgcga
    10441 atttcgcctg ctggtcagaa acgccgaatt catcgggaca gaacgaaatt tcttccgcat
    10501 cgccctcatt caggtacgca tcggatactg gctttttttc ctgagatttt ccgttccgct
    10561 tttgtgcagt ctgcgcagat tttttctgcg cacttttttg cgcagttttg cgcatttctg
    10621 tctgcgcatt tttcggaggt tttttgatgt aacgacgggc tgttgcgtaa ttcagtcccc
    10681 ttgcttcaca ccatgccacc ggagatatac cggagcgggt gtattcagca atatactcct
    10741 gctgcaacgc cccccagtcc ggtctgctca tcagttagtc ctgattttta tccaccctga
    10801 gtagttcgcg cagggcaaag gcatcccctt ttctggcaag cttaaacaat gccgcccgta
    10861 gctcggcttc acctttcgct ctgcccttac ggatggacgc gtaaaaattt gtcattgctt
    10921 cccgattttc tttcagtcgg ttcagatcaa catccagaac gtcagcgatt tgttgtgcag
    10981 tcatccggca cgctgccaga gactcgactt tcgaatacgg aatcatttgt cacccccatt
    11041 aatatgcagg gtgtcttctt cctgtatttt tcgtgaagga tttttactgc agcgttgttc
    11101 caggtgacct gatggtgaat gcgtttatgg ctggcgccca tcagtgagat ttttacgcac
    11161 gacggcgcat acatgacgga ataaaaactt ttaacgtagg tcccggaatc cagatacagc
    11221 tcggtcattc cgccgctgtt tttctgcgtc tgtttctgcc ctaactggac agcaccgatc
    11281 gtcataaaca attcaccacg gcgaccgaga ttcgtgtacg tattcacatc ctcgttaatg
    11341 cgccccatga atgagaacgg tcgatcaacc gaacagataa agctgttcat tgccttgcgt
    11401 ttcacccacg aagcatggcc gccattgtca ccaagaaaat ccccgccctg cgacatagcg
    11461 atggaaagcg cgggtattga ttcgtaatac gccagcattt cagaaaggat cgcatccagt
    11521 ttccttatcg ggaaataggc ctggtcatag ttgcgatcca cccgaaactg gaactcgtga
    11581 taatcatcat cgagctgaat gaagtattta cacccgacca gttttgccag gtcgaaacag
    11641 gcattacggg cgtaaaaaat tgagcggtgg tcaccaaaat tatcggcttc gtcaaaacga
    11701 ctggcaatat cggctttgga aaataccagc acctgttcac caaattcagc catgtactga
    11761 tgccgtgtct tatcttcatc atcaacaacg ataaaaattt tcccggtata gccagcacga
    11821 cgcaacgtcc ggtaagtcag aactttgtcc ggtcgcccgt gagtcagaat aaaggcgcaa
    11881 aaatcatcac acatattcct cctccccgct atgcatgatc gccaccatgc gctgcgtcat
    11941 tcggacaaat ccattttcaa tagcctgctg ataatcaatg atcaccagcg ccgactcctc
    12001 gaaaaggcac tgaatttcag cgggggcatg agcgtaatag tccgcaattc tgctgaaatt
    12061 aaacaccgtg tgacgttctg ccgcacacag gaggaatttc tcaatatcag gatcaaggga
    12121 cgccgaacgt atccggctga tcagctcctg agttttcgta tcgtcataca gttcactgat
    12181 atccggttta ccgcccgacg gctcataaac aggcgtatca attttcgtcg tatacggctc
    12241 ctccttattt cctgtaccgg gcaaaacatc cgtcaacagt tcatcaattt ctgtcgggcc
    12301 gaagcctgtc agggagacat caaaatcagc attgattagg tccgacagct ccatccgcaa
    12361 cagatcttca tcccagccag cattcatcgg taggcgatta tctgccaggc ggtacgcctt
    12421 tttctgctca tccgtcaggc cagacagaac aatgaccgga acagaatcca ttttgagcat
    12481 ttcagccgcc ataacacgac cgtgacccgc aataatttcg cccttttcgt caatcagcac
    12541 cggattagtc cagccgaatt gcttaatact ttctaccagt tgtgccacct gctcagtact
    12601 gtgcgtcctg gcgttgtgcg catacggtga caattcttgt aatgggcgat agactatctt
    12661 taatttctcg ctcatacagc cttgctttat gaataaaacg caccccagca gccagtgcta
    12721 ctggggcgga ggtgttgctg gtaaagttag gtattggatc aatgagtgag tcaatataat
    12781 attaaactca caattataaa tcagccatat attaggagcg ccaaaaaaaa cctgaaaaca
    12841 atataataac aggataaatt tcaaggcgac caagaatcat agctatgcac attaaatatt
    12901 ttgcaatgtc attaagcact ccgaatgacg atgcagtagc cccaaaacct aatcccatat
    12961 tattaataca tgcagccact gttgcaaatg atgtaagaaa atcatatccc ataccattta
    13021 acaccagtat aaaaaacacc gtgaagagag tataaagaaa aaagaaactc catacagacc
    13081 tcattacacg atctgtaact atcttccctc ctacatttac actcaacaac gctctgggat
    13141 gagaaagctg atttatctcg tgtttgcttt gtttgaaaag tataagaaat cgaagtgact
    13201 taattccacc acaagttgaa cctatacatc ccccaaagaa acttgacgac agcaaaaaca
    13261 ctatcgtgtg cgtgggccag tttgcataat cctgcgtagc taaaccatta tcagtgagca
    13321 tggagctggc aagaaaaaac gaatgaataa aacttccatg caagtcatac atacctatat
    13381 gccagacctg gaaagaggta acaatgatca cccctaaggc tattaacaga aagaaacgaa
    13441 gttcaatatc tctgattaaa ggttttatcg ttttcctgct aataacaata taccaaagag
    13501 tgaagttgaa agccgatagc agggaaaaag aaccagccac cagctcaacc aaatagttat
    13561 taaaatatcc gatactctcg ctatgagttg agaaaccacc aagcgaaact gtggaaatcc
    13621 cgtgacaaat agcatcaaac aaaggcattc ctgcaagtct ataacagaca atacaagcaa
    13681 tacctaataa agaataagtt atccacagtg tccgtgacgt atcggccagg cggggagtga
    13741 gtttgtcatc cttaaatggc cccggcattt ctgactgata aagctttgca ccaccaatac
    13801 ccaataatgg caatacagca accgccagaa caataactcc taaaccacct ataaaattta
    13861 actgtgaccg atagtacaaa tatgcccgag gtaatgaact aacatcatca attacagttg
    13921 ctcctgttgt tgttattcca gaaacccctt caaacagagc atcaatgaac gttaaattaa
    13981 gttctgagtc aatccataaa gggaatgcac taataacaga aaacaaaatc caaaacatta
    14041 caattataat aaacccatca cgggtacgta attgaatgcc agatttctta gttgtatacc
    14101 acgctccgcc accaatgcaa aaaaacataa cgaaagttat aaagaaaacg aacaggcttt
    14161 tttctttata aaacaatgct acaaccattg gtggcaacat tgaaagacta tagagccaaa
    14221 ccagaaaccc acacatatga gtaacaactc ttacatgaga tgtattcata tctaaatatt
    14281 ctttcaatta taaccacctt gctgcaatat tatgattata ctgtataaaa tttaactcct
    14341 cttagatctt acttcactgt tccttatgaa acaatcatca aaatgaatca tattgtagtt
    14401 aagattttac tttaaacact gttcggttat gtattgctga gcaccttcaa gttgggcctg
    14461 catcattacc agtcgttccc ggagggtgaa ataatcccgt tcagcggtgt ctgccagtcg
    14521 gggggaggct gcattatcca cgccggaggc ggtggtggct tcacgcactg actgacagac
    14581 tgctttgatg tgcaaccgac gacgaccagc ggcaacatca tcacgcagag catcattttc
    14641 agctttcgca ttagctaact ccttcgtgta tttttcatcc agtgcagcaa catcacgctg
    14701 gcgcatctgc atgtcagtaa ttgccgcgtt cgccagcttc agttctctgg catttttgtc
    14761 gcgctgggct ttgtaggtaa tggcgttatc acggtaatga ttaacagccc atgacaggca
    14821 gacgatgatg cagataacca gagcggagat aatcgcggtt actctgttca ttgctgaccc
    14881 cacaaacaga tttcacgctc aatctcacga cgagtcatga gacctttcca ttgcttaccg
    14941 ccagcatatg tccagcgacg tagctgatca catgcgcctt tgatatcgcc ctggtttatt
    15001 ttgcgaagaa gcgtcgatgt tctgaaattg ccagcaccca cgttgtaaac gaatgagtaa
    15061 agagcgccgc gcgttgtttc cggtatatcg acttcgatgt acgggttaat ttgtctggcg
    15121 acagtggcaa ggtctttatt caagagtgct ttgcattctg ctttggtata cgttttaccg
    15181 agcatgatgt cttttcctgt atgcccgtga catacagtcc atacaccaac aatatctttg
    15241 tatggtatgt agctgacacc ttccagacca tcgttaccac ttgggccagt gattaacact
    15301 gatgctatag caattgctcc gccaccaata gcagcagcaa cggcttttcg taatgatgga
    15361 ggcattattc acctctcgca gccttgcgct tatcttcttt aatcttgaaa taaaggtttg
    15421 tcaggtacgt cagcaggcca aaaaccagac tacccagcac accgattgca gcccactgtg
    15481 acggagttac tctatcgagc aactgtaaaa accagtagcc agcactgcct gcggaggtgc
    15541 cataggcgac acccgttgtt aacttatcca tggatttcat aacccccacc tcgcagatgc
    15601 gggcgctgtg taatggaaac aaaaaatggc caccagcggc ccgtaaaaaa caccccgtca
    15661 aaagcaccgg catccgcaga tgccctttgc gtggcgttat ttgatgcgcg ccagatgtgg
    15721 cgcaaagaaa tgaaataaga cttatcgaaa attaaggtta atttgatgat ttaaaccact
    15781 tctgaagctt agtagtatga acatgtcccc agaagggggc caatacttat gattcttcat
    15841 ggactttgtc ccgcggcgtt aatccgacgg ccgcgctttt tttgcgccat attcatcgct
    15901 aacttacatg gcattgccac acgggcatta tcagtgtccg tgtttctttt tttagggttc
    15961 agaataaaaa aaaccgcccg gtggaggcgg taaaggatgt atttccaggt tttgcttagt
    16021 atatgattaa tctcaatgtc atttttagcg atgtttacaa catcgggatg gcacatcacc
    16081 ggccctgcca ggaaatacaa aatctccacc gataatgcac cattctgctg ccgtaaaaaa
    16141 atcagcactg aggctacacc tggcctcaaa tcatagccag agaacagaat gctttttcga
    16201 aacaacctgc tcccacgtaa taaaaatacg ccagtgctgc aatacaataa ggcttatttc
    16261 aaatgctgga gcgggtagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa
    16321 taaccattat acgatacccg catatggtgc cgactaccgg aatcgaactg gtgacctact
    16381 gattacaagt cagttgctct acctactgag ctaagtcggc acagaaccgc caccgaggta
    16441 tcgaatctca cacctaccac ttaaatgatg gtcggctctt cccgatgagc agatggcggt
    16501 gttcccttaa tgggatagct gtattcagta atccgttatt cgaatacacc atcgaaggaa
    16561 caagcgcatt atatacattc acgattcgtg ttattagct
//
LOCUS       DACAPB010000094        16533 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.094, whole genome shotgun sequence.
ACCESSION   DACAPB010000094 DACAPB010000000
VERSION     DACAPB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.094"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..930)
                     /gene="hemL"
                     /locus_tag="IT904_002745"
     CDS             complement(<1..930)
                     /gene="hemL"
                     /locus_tag="IT904_002745"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAP3192504.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFA"
     gene            1155..2576
                     /gene="clcA"
                     /locus_tag="IT904_002746"
     CDS             1155..2576
                     /gene="clcA"
                     /locus_tag="IT904_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAP3192505.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            2600..2665
                     /locus_tag="IT904_002747"
     CDS             2600..2665
                     /locus_tag="IT904_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192506.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            2658..3002
                     /gene="erpA"
                     /locus_tag="IT904_002748"
     CDS             2658..3002
                     /gene="erpA"
                     /locus_tag="IT904_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAP3192507.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3049..3672)
                     /locus_tag="IT904_002749"
     CDS             complement(3049..3672)
                     /locus_tag="IT904_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAP3192508.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3710..4510)
                     /gene="btuF"
                     /locus_tag="IT904_002750"
     CDS             complement(3710..4510)
                     /gene="btuF"
                     /locus_tag="IT904_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAP3192509.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(4503..5201)
                     /gene="mtnN"
                     /locus_tag="IT904_002751"
     CDS             complement(4503..5201)
                     /gene="mtnN"
                     /locus_tag="IT904_002751"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP3192510.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            5285..6802
                     /gene="dgt"
                     /locus_tag="IT904_002752"
     CDS             5285..6802
                     /gene="dgt"
                     /locus_tag="IT904_002752"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAP3192511.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            6932..8356
                     /gene="degP"
                     /locus_tag="IT904_002753"
     CDS             6932..8356
                     /gene="degP"
                     /locus_tag="IT904_002753"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAP3192512.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            8511..9668
                     /locus_tag="IT904_002754"
     CDS             8511..9668
                     /locus_tag="IT904_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAP3192513.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPVLNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKTMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(9757..10143)
                     /locus_tag="IT904_002755"
     CDS             complement(9757..10143)
                     /locus_tag="IT904_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAP3192514.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(10305..11117)
                     /gene="yaeI"
                     /locus_tag="IT904_002756"
     CDS             complement(10305..11117)
                     /gene="yaeI"
                     /locus_tag="IT904_002756"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HAP3192515.1"
                     /translation="MISRRRFLQATAATIATSSGFGYMHYCEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMSLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(11171..11995)
                     /gene="dapD"
                     /locus_tag="IT904_002757"
     CDS             complement(11171..11995)
                     /gene="dapD"
                     /locus_tag="IT904_002757"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAP3192516.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(12026..14698)
                     /gene="glnD"
                     /locus_tag="IT904_002758"
     CDS             complement(12026..14698)
                     /gene="glnD"
                     /locus_tag="IT904_002758"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAP3192517.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECILEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGNPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(14760..15554)
                     /gene="map"
                     /locus_tag="IT904_002759"
     CDS             complement(14760..15554)
                     /gene="map"
                     /locus_tag="IT904_002759"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP3192518.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            15922..>16533
                     /gene="rpsB"
                     /locus_tag="IT904_002760"
     CDS             15922..>16533
                     /gene="rpsB"
                     /locus_tag="IT904_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP3192519.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGND"
BASE COUNT         3978 a         4157 c         4361 g         4037 t
ORIGIN      
        1 ggcgaaaccc gctgccatcg caatcgggtt accggaaagc gtacccgcct gatagaccgg
       61 acccgtcggg gccagcgcat ccattacatc acgacgacca ccgaatgcgc ctaccggcat
      121 tccaccgccg atgattttgc cgaggcaggt taaatctggc actacgccgt aataatcctg
      181 tgcgccagct agcgctacgc ggaaaccggt catcacttca tcgatgatca gcaacgcgcc
      241 aaattcgtcg cacagcgcgc gcagacctgg caggaactct ggcagcggcg gaacacagtt
      301 catattgcct gccaccggct cgacgataat acaggcaatc tcttgcgggt attgctcaaa
      361 tgcggcgcgt acagaagcca gatcattata agtacaggtt aaggtatgtt tggcgaaatc
      421 tgccggaacg cccggcgagt ttggctggcc taacgtgagt gcgccagaac cggctttcac
      481 cagcaggcag tcagcgtgac catggtaaca cccttcaaat ttaataattt tgtcgcgacc
      541 ggtaaaacca cgggccaggc ggatggcgct catggtcgct tcagtgccgg agttcaccat
      601 gcgcaccata tccatggtcg ggaccagttc ggtcaccagt tgcgccattt tcacttccat
      661 ttcggttggt gcaccaaagc ttaaaccacg ctcggcggct tcaatcacgg cattgcggat
      721 tgccggatgg ttatggccca gcaccatcgg cccccaggaa ccgacataat cgatataggc
      781 tttgccatca acatcgtaca gataagcgcc gtccgctttt tcgataaaca gtggagtgcc
      841 gcccacgcca gtaaaggcgc gaacagggga gttcacaccg ccagggatca gctcgcgcgc
      901 tgcgctgtaa agattttcag acttactcat ggagggttcc tgattcgtag aaaaagtgaa
      961 tggctgctat tctatgttat tcataacaag ttaaatactc gtcaaacatc aggctgcttg
     1021 tactggtgca atcctgattt cttagagtat aaaagttttg tgcatttgaa acattacgct
     1081 ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt catcttattt gtatgaccaa
     1141 taagtgatca ttggatgaaa actgatactc cctctttaga aacaccgcag gccgcgcgcc
     1201 tgcgacgcag acaactgatt cgccaacttc ttgagcgcga taaaaccccg ttagccattt
     1261 tgtttatggc ggcagtcgtc ggcacgcttg ttgggctggc agcggttgct tttgacaaag
     1321 gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt acatactgct gataattatc
     1381 cgcttctgtt aaccgtcgct tttctctgtt cggcggtgct ggcgatgttt ggctactttt
     1441 tggtgcgcaa atacgcgccg gaagcaggtg gttcggggat cccggaaatt gaaggggcgc
     1501 tggaagatca acgtcccgtt cgctggtggc gtgtattgcc ggtgaagttc tttggcgggc
     1561 tggggacact cggcggaggc atggtgttgg ggcgcgaagg gccaaccgtg cagatcggcg
     1621 gtaacattgg ccgtatggtg cttgatattt tccgcctgaa aggtgacgaa gctcgccata
     1681 cgctgctggc aaccggtgct gctgcggggc tggctgcggc ctttaacgcg ccgctggcgg
     1741 gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg ctatacgtta atttcgatta
     1801 aagcggtatt tattggtgtc attatgtcga ccattatgta ccggattttt aatcatgaag
     1861 ttgcgttgat tgacgtcggt aaactttctg atgcgccgct taatacgctg tggctttatc
     1921 tgatcctcgg tattattttt ggcattttcg gccctatttt taataaatgg gtgctgggga
     1981 tgcaggattt gctgcaccgt gtgcacggcg gcaatattac caaatgggtg ctaatgggcg
     2041 gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc accagcaacg tcgggcggcg
     2101 gttttaacct gattcctatc gctaccgcgg ggaatttcag catgggaatg ctggtgttta
     2161 tcttcgtcgc gcgggtcatt accaccttac tctgcttctc ttccggcgcg ccgggcggta
     2221 tttttgcccc gatgctggcg ctgggtactg tgctgggaac cgctttcgga atggttgccg
     2281 ttgagctgtt tccgcaatat caccttgagg cggggacgtt tgctattgcc ggaatggggg
     2341 cattactggc ggcatctatt cgcgcgccgt taacggggat cattctggtt ctggagatga
     2401 ccgataacta ccagctcatt ttgccaatga ttattaccgg tcttggcgca acactattag
     2461 cgcaatttac cggcgggaaa ccgctatact cggcgattct tgcgcgcacg ctggcaaaac
     2521 aggaagctga gcaactggcg cgaagcaagg ccgcatcagc cagcgagaat acttgaacga
     2581 aataccaggg tattagataa tggcgattat tattgggtta gaatttgccc aattgccgat
     2641 gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc gctggagttt accgacgcag
     2701 cagccaacaa agttaaaagc ctgatcgctg acgaagataa cccgaatctg aaattacgcg
     2761 tgtatatcac cggtggcggt tgcagcggct tccagtatgg tttcaccttt gatgatcagg
     2821 tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg cctggtggtt gatccgatga
     2881 gcctgcaata tctggtcggc ggttccgttg attataccga aggtctggaa ggttctcgtt
     2941 tcatcgtgac caacccgaac gcgaaaagca cctgcggttg cggttcttcc tttagtatct
     3001 aatctgttgt tcatgtgccg gataaactat ccggcacatc ttctctcttc aacgcccatt
     3061 ctcatccagc gcaaacgtcg gtagcttaag atgccaacga atagccgcca gccgaatcaa
     3121 tagcgtcacg accatgccca tcatactggc tgtttccagt ggtacggaaa atgtgtaata
     3181 agccgtagcg tggacaatac cgccgataat acaggcagtt gcgtagattt ctgtacgtaa
     3241 aatcatgggg atttcgcggg ccagaacatc acgaatgatc ccgccgccaa cgccagtaat
     3301 gacgcccata caaaccgcga ttaacggacc ggcttccgca ttaaaggctt tattcacgcc
     3361 aatgccgaca aacaccgcca gaccaacggc gtccagcacc ggcaacatcc attttggtaa
     3421 gcgtcttggc tggcgcacca gcacgatggt cagcatgctg gtgaccattg caacgaccag
     3481 atcggtggga tctttcaccc aaaataccgg gccgtgatcc agcgccatgt cgcgaattgt
     3541 cccgccgcct acagcggtaa ccacgcccag taccagaaca ccaaaagggt ccatacgcaa
     3601 ttttccggct aacaaaacgc cggagatggc aaataccgct gtgccgacta tatccagcca
     3661 atagacgagc attgttcaat ccccactgag cacctgaaaa ggtctggcgc taatctacct
     3721 gtgaaagcgc attacagagc tgttgtgcag cgaggataat acgtgggctt gcacgttcaa
     3781 accagtcact cgtgagagga ataacgggaa ttttgagctg ttcaccccag tattgtttga
     3841 ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc ctgtggcgag cgtgctaaca
     3901 cctgttcgcg gctaacttgc ggccagggaa cccggctgtc tttaaagatg ttttctccgc
     3961 cacaaacttc gagtacctgg ttttgaatcg actcttttcc actggtaaat ggcggattaa
     4021 tgccgaattg cagaaaaaca cgttttttag gtttatcagc atattgcgct ttcaattgcg
     4081 cgtactgatc cagcagggat tgcgcggctt gttcggcctt atccggttgc ggactccagg
     4141 gggccagttg acgtaacgca ttggcaattt gttcaatgct tgtcgcatcg acccacatca
     4201 cttttattcc cagcgaagcc agctggtcaa cctgccgctc ggcattacct ccacgccagg
     4261 caatcaccag atcgggtttc agcgcgacaa tgcgttccag attcatcccc tgccaagtgg
     4321 aaacctgctc aatcttttgc gcttgtggag gatagtcgga atagctgctg accccaaccg
     4381 gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg agaaagcgtg atgacgcgcg
     4441 gcgcggcgtt gagccacagt ggcgcgagaa aagacagggc gaccagcgcc ctgaacagtg
     4501 acttagccat gtgccagttt ctgcaccagt gactcaacca tcaggctgga ctgtttagcg
     4561 gcaacagcca ggaactcatc gaagctaaga tgagactgtt gatcggccac gtcggagatg
     4621 gcgcgtacta cgacaaacgg gacgttgaaa ttgtggcaga catgggcgat tgccgtcgct
     4681 tccatctcta cagcaatggc ctgtgggaag ttgtggcgga ttttcgccag accaacagaa
     4741 ccgttgatga aagcgtcgcc gctaacaatc aggccacgta cagcgttaag attcagttcg
     4801 gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag ctttaaagcc tgccggacag
     4861 cctggtaact gaccgtattc ataaccaaat gccgtgacat ccgcgtcgtg ataacgtgct
     4921 tcgtccgaga caacgatatc gcccactttc aacgttggtg ccaggccacc ggcagaaccg
     4981 gtgttaataa tcacatctgg cttgcagtgt tccaacagca gagtggcacc cagcgccgca
     5041 gcgactttac cgatgcccga tttcagaagc gcaacctcgg ttccattcag ttggccggta
     5101 tagatttcgc aaccgccgag actgatagtt tgacggtttt cgattttgtc acgcagcagc
     5161 gtaacttctt cttccattgc accaatgatg ccgattttca tagatttact cgcgataagc
     5221 ccgatttgaa ggcatagttt accatgcgct tacggggaag cgtatttctc acgcgggaga
     5281 ggacatggca cagattgatt tccgaaaaaa aataaactgg catcgtcgtt accgttcacc
     5341 gcagggcgtt aaaaccgaac atgagatcct gcggatcttc gagagcgatc gcgggcgtat
     5401 catcaactct ccggcaattc gtcgtctgca acaaaagacc caggtttttc cactggagcg
     5461 caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa gtccagcagg tgggacgcta
     5521 catcgccaaa gaaattttaa gccgtctgaa agagcttaaa ttgctggaag catacggcct
     5581 ggatgaactg accggcccct ttgaaagcat tgttgagatg tcatgcctga tgcacgatat
     5641 cggcaatccg ccgtttggtc attttggcga agcagcgata aatgactggt ttcgccaacg
     5701 tttgcacccg gaagatgccg aaagccagcc tctgactgac gatcgctgca gcgtggcggc
     5761 actacgttta cgggacgggg aagaaccgct taacgagctg cggcgcaaga ttcgtcagga
     5821 cttatgtcat tttgagggga atgcacaagg cattcgtctg gtgcatacat tgatgcggat
     5881 gaatctcacc tgggcacagg ttggcggtat tttaaaatat acccgtccgg cgtggtggcg
     5941 tggcgaaacg cctgagacac atcactattt aatgaaaaag ccgggttatt atctttctga
     6001 agaagcctat attgcgcggt tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc
     6061 attaacgtgg attatggaag ctgccgacga catctcctat tgtgtggcag accttgaaga
     6121 tgcggtagag aaaagaatat ttaccgttga gcagctttat catcatttgc acgaagcgtg
     6181 gggccagcat gagaaaggtt cgctcttttc gctagtggtt gaaaatgcct gggaaaaatc
     6241 acgctcaaat agtttaagcc gcagtacgga agatcagttt tttatgtatt tacgagtaaa
     6301 caccctaaat aaactggtac cctacgcggc acaacgattt attgataatc tgcctgcgat
     6361 tttcgccgga acgtttaatc atgcattatt ggaagatgcc agcgaatgca gcgatcttct
     6421 taagctatat aaaaatgtcg ctgtaaaaca tgtgtttagc catccagatg tcgagcagct
     6481 tgaattgcag ggctatcggg tcattagcgg attattagag atttatcgtc ctttattaag
     6541 cctgtcgtta tcagacttta ctgaactggt agaaaaagaa cgggtgaaac gtttccctat
     6601 tgaatcgcgc ttattccaca aactctcgac gcgccatcgg ctggcctatg tcgaggctgt
     6661 cagtaaatta ccgtcagatt ctcctgagtt tccgctatgg gaatattatt accgttgccg
     6721 cctgctgcag gattatatca gcggtatgac cgacctctat gcgtgggatg aataccgacg
     6781 tctgatggcc gtagaacaat aaccaggctt ttgtaaagac gaacaataaa tttttacctt
     6841 ttgcagaaac tttagttcgg aacttcaggc tataaaacga atctgaagaa cacagcaatt
     6901 ttgcgttatc tgttaatcga gactgaaata catgaaaaaa accacattag cactgagtgc
     6961 actggctctg agtttaggtt tggcgttatc tccgctctct gcaacggcgg ctgagacttc
     7021 ttcagcaacg acagcccagc agatgccaag ccttgcaccg atgctcgaaa aggtgatgcc
     7081 ttcagtggtc agcattaacg tagaaggtag cacaaccgtt aatacgccgc gtatgccgcg
     7141 taatttccag cagttcttcg gtgatgattc tccgttctgc caggaaggtt ctccgttcca
     7201 gagctctccg ttctgccagg gtggccaggg cggtaatggt ggcggccagc aacagaaatt
     7261 catggcgctg ggttccggcg tcatcattga tgccgataaa ggctatgtcg tcaccaacaa
     7321 ccacgttgtt gataacgcga cggtcattaa agttcaactg agcgatggcc gtaagttcga
     7381 cgcgaagatg gttggcaaag atccgcgctc tgatatcgcg ctgatccaaa tccagaaccc
     7441 gaaaaacctg accgcaatta agatggcgga ttctgatgca ctgcgcgtgg gtgattacac
     7501 cgtagcgatt ggtaacccgt ttggtctggg cgagacggta acttccggga ttgtctctgc
     7561 gctggggcgt agcggcctga atgccgaaaa ctacgaaaac ttcatccaga ccgatgcagc
     7621 gatcaaccgt ggtaactccg gtggtgcgct ggttaacctg aacggcgaac tgatcggtat
     7681 caacaccgcg atcctcgcac cggacggcgg caacatcggt atcggttttg ctatcccgag
     7741 taacatggtg aaaaacctga cctcgcagat ggtggaatac ggccaggtga aacgcggtga
     7801 gctgggtatt atggggactg agctgaactc cgaactggcg aaagcgatga aagttgacgc
     7861 ccagcgcggt gctttcgtaa gccaggttct gcctaattcc tccgctgcaa aagcgggcat
     7921 taaagcgggt gatgtgatca cctcactgaa cggtaagccg atcagcagct ttgccgcact
     7981 gcgtgctcag gtgggtacta tgccggtagg cagcaaactg accctgggct tactgcgcga
     8041 cggtaagcag gttaacgtga acctggaact gcagcagagc agccagaatc aggttgattc
     8101 cagctccatc ttcaacggca ttgaaggcgc tgagatgagc aacaaaggca aagatcaggg
     8161 cgtggtagtg aacaacgtga aaacgggcac tccggctgcg cagatcggcc tgaagaaagg
     8221 tgatgtgatt attggcgcga accagcaggc agtgaaaaac atcgctgaac tgcgtaaagt
     8281 tctcgacagc aaaccgtctg tgctggcact caacattcag cgcggcgaca gcaccatcta
     8341 cctgttaatg cagtaatctc cctcaacccc ttcctgaaaa cgggaagggg ttctccttac
     8401 aatctgtgaa cttcaccaca actccataca tcttcatcat cctttaggca tttgcacaat
     8461 gccgtacgtt acgtacttcc ttatgctaag ccgtgcataa cggaggactt atggctggct
     8521 ggcatcttga taccaaaatg gcgcaggata tcgtggcacg taccatgcgc atcatcgata
     8581 ccaatatcaa cgtaatggat gcccgtgggc gaattatcgg cagcggcgat cgtgagcgta
     8641 ttggtgaatt gcacgaaggt gcattgctgg tactttcaca gggacgagtc gtcgatatcg
     8701 atgacgcggt agcacgtcat ctgcacggtg tgcggcaggg gattaatcta ccgttacggc
     8761 tggaaggtga aattgtcggc gtaattggcc tgacaggtga accagagaat ctgcgtaaat
     8821 atggcgaact ggtctgcatg acggctgaaa tgatgctgga acagtcgcgg ttgatgcact
     8881 tgttggcgca ggatagccgt ttgcgggaag aactggtgat gaacctgatt caggcagagg
     8941 agaatactcc cgcacttact gaatgggcgc aacggctggg gatcgatctc aatcaaccgc
     9001 gagtggtggc tattgttgag gtcgacagcg gtcagcttgg cgtggacagc gcaatggcgg
     9061 agttacaaca actgcaaaac gcgctgacta cgcccgagcg taataatctg gtggcgattg
     9121 tctcgctaac cgaaatggtg gtgttgaaac cggtgttgaa ctcttttggg cgctgggatg
     9181 cagaagatca tcgtaagcga gttgaacaac tgattacccg catgaaagag tacggccagc
     9241 tgcgttttcg cgtttcactg ggcaactatt ttaccggtcc tggcagtatt gcccgatcct
     9301 atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg gatgccagaa agtcgctgct
     9361 atttttatca ggatctgatg ttacctgtgt tactcgacag tttgcgtggc gactggcagg
     9421 ccaacgaact ggcgcgaccg ctggcgcggc tgaaaacgat ggacaataac ggcttgctgc
     9481 gacgaacgct ggcggcgtgg tttcgccaca atgtgcaacc gctggcaacg tcaaaggcgt
     9541 tgtttattca tcgtaatacc ctggagtatc ggcttaatcg tatatcggaa ctgaccgggc
     9601 ttgatttggg caattttgat gacaggttgc tgctgtatgt ggcgttacaa ctggatgaag
     9661 agcggtaggt tatgcgttaa ggttgaggcg gcgctggctc attcccctca gcccgaaggg
     9721 tagaggggaa tgcccgaatt aaccgtcagt tcgggattat ttacgcgtta gtttttccag
     9781 atcggcttca atctcgctga tcttattggt tacgactgac tccaggtgac gtaagtcgtc
     9841 gaggatctta cgcttaagat caacttcgct gcggtcacgc tggcagattt gatcaagctc
     9901 atcaatgata taccgtagat tcgggctgat ttcctggact tctttataac cctgacccac
     9961 accatcagcg acgaccgttt tacgctgacg cggatattta aacttaacgc tcttggcgaa
    10021 aaactcgcct ttgtctttct ggaaatagat tttcagaata tcgttgttgg cttcctgccg
    10081 gaggctgtaa cgatcaattt cttcaggatt ggtaataccc agacttttca gattgtcgta
    10141 catagcgtta cctcaaaatg agtcagtaaa ttgtgcttat tttagcattt ggcctggccc
    10201 gcccggctcg actgtgatcg cagaaagcct ttttcttcca gacttctatc atggcgtaaa
    10261 aatcaaaaaa ttacctgctt tattctggtg ataaaattca cgatctacac cagttccagc
    10321 atcgttactt ccgggcggca gttcagacgc aaaccataca aactgcccac gccacgggtt
    10381 gtgtagatgt gtctttcgcc aaaggcattt aatccggcga cgtaacgttt atcttcgaca
    10441 ggggcaaaag gttcaccaac caacggtacg cgcagttgcc cgccgtgagt atggccgcac
    10501 agcatcagat cccagggttc atcacgcatg acttctttgc tgtcgggatt atgcgccagc
    10561 accagtcttg gcagattggc ttcgctggcg ggaggcggtt tgcattgtcc ggcccataag
    10621 tcaccagtgc ctaccagttc gaattgcctg ttcggcgtgg cgatcaccgt agcctggtta
    10681 aacaacaccg tgatgcccgc tgatttcaac gtctcgccaa ttaagtgatt tttttctgta
    10741 ccaacagggc gatcgtggtt gccgaagcag gcaaacgtcg gcgcacattc ggcaaggggg
    10801 gagagtacgt cactaaacgc cgaaaaattc agcgacatat caaataatac gtaatcgccg
    10861 cccagtaata tcaaatcggg cttttgttct atgccaagag caatcgcgtc agaaatcagg
    10921 cttaaaggaa caaaacgaga gtaatggaga tcggccagaa aaagaatttt gaatggtgct
    10981 gcgttgtctt taaaaaaggc gaggcggtga cggattaatt caaaccagcc tggctcacag
    11041 taatgcatat aaccgaaacc tgagctcgtg gctatcgtcg cggcagtagc ctgcaaaaat
    11101 cggcggcgtg aaatcatcgc tcatccctgc aaaaaaatcg ggcagcgtcg tgctgcccgt
    11161 gtgcatactt ttagtcgatg gtacgcagca gttcgttaat gccgactttg ccgcgagttt
    11221 tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca tcttttgacg
    11281 gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg atttcgccgg
    11341 tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatggaa atgacggaac
    11401 cttcttcgac aatcacccct tcaaccactt cagagcgcgc gccgatgaag caattatctt
    11461 caatgatggt tgggttagcc tgcagcggtt ccagcacgcc gccgatgccc acgccaccgg
    11521 aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag gtatcaacca
    11581 tggtgccttc atcaacatat gcgccgatgt tgacgtaaga cggcatcagc acggtgttac
    11641 gggcaataaa cgcaccctga cgtaccgccg ctggtggcac aacgcggaag ccttctttct
    11701 ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt
    11761 ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc gcttttttca
    11821 accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat
    11881 ccagcagggc gatcacctga tttaccgctt cgcgggtaac ggtgtctgca ttggctggcg
    11941 tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc tgcattgtta
    12001 aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg gcctctgtca
    12061 accgctgatg cacttcctgc tgcaactcgt tattaagcgc acgccggtca gcggtggcaa
    12121 ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa
    12181 ttcccagatc ggcaaaaatt ttcccgactc gcgccagcag tccaggttgg tcgagggcga
    12241 tcagttcgag gaacgatttg cggtcggtat gggtcggcaa aaacgttact tcggtttcaa
    12301 cagtaaaatg gcgtaatttg gcgggttggc gacggggctg cggtggctgc cagctacttt
    12361 gcgtcagtac ttgctccaga ccaaaccgaa taacctcatg acgatctgcg gacagcgggt
    12421 tgccatcggg ttccagcacg ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa
    12481 tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg gcggcaaaca
    12541 gataagggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc
    12601 taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca tgccaggcca
    12661 gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa atttggtgca
    12721 gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt tggtgatggc
    12781 gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg
    12841 caaagtagag ctcacgcaac agactttgct tccagctatt ccacagcgtt tcgttggtgg
    12901 cgcaaatgtc agccacagtc aggcatacca gatagcgcag acgattttcc gtttgcactt
    12961 cttcggcaaa ctgcttgatg acttccgggt cctgaatatc gcggcgttgg gcggtcaccg
    13021 acatcaacag gtgctggcga accagccacg ccaccagttg cgtttcgcgc gagttaagcc
    13081 cgtggagttc ggcaaaatgc actacatcct gagcaccgag aatggagtgg tcgccgccgc
    13141 gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt gacggtaggc
    13201 gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg gcaaaactct
    13261 ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg aacagatcaa
    13321 actgcatctg cccgacgata tgtgaccatt gcggcatata cgcgccgagt acgctatggc
    13381 gatgcattgg caatagcccg cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca
    13441 gttttcgtgc ttccggaata ttacacagcg gttgttgcag atggcgccgg gcatggcgta
    13501 actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc atggtgtaga
    13561 acatacgtaa gatagcttcc ggctggcgca taaatagtgt ttcatcacgc aggtcgatta
    13621 gcgtaccgcg tagctgaaac tcatcgtcga ttggacgtgg tttttcgtcg gcgggaaggg
    13681 cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg acgcggcgtg
    13741 tcacgcggaa gtaatccttc atcatccgct cgaccggttc gttaccttcg ccactgtaat
    13801 tcagacgctg ggcgacgcta agctggcgat cgaataacag gcgattatcg taacggctga
    13861 cgaccagatg cagggcaaag cgaatacgcc acaatatatg cagacattcg tttaattccg
    13921 cccgctccgc tgaggttaag aagccaaacc caaccatttc atccagcgat gttgcgccaa
    13981 aatgacggcg ggccacccat tgcagagtgt ggatatcgcg caagccgcca gggctacttt
    14041 tgatgtctgg ttcaagattg tagctggtgc cgtggtaacg ctgatggcgc tggttctgtt
    14101 cttcaacttt cgccgcgtag aacttgtcgg aaggccagaa tccttcgctg aaaatatgtt
    14161 tttgcagttc aagaaatagc gcaacatcgc caattaataa gcgggattcg atcaaattgg
    14221 tggcgacggt taaatccgat aacccttcca gaatgcactc ttcaagcgtg cgcacactat
    14281 gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact ttttgcgcct
    14341 gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca
    14401 gctcgccacg accgtagcca ccgacggcga ccaatgccag gtcggcaatc tggctgaatc
    14461 ccgcttcaat ccataatcgt tgcaggagct ggtcgataaa ctcggtgcgc gcctcaatca
    14521 actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg aaagtatcga
    14581 tatgggcttt tatcccaccg acggttaatt catcacgggg ccagacgcat ggattttgcg
    14641 gttgaccggg cagggtgggg agagcggtgt ttgcgtactg ttctggaagg gtattcattg
    14701 tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc ggcttcatct
    14761 tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt cagaatttcg
    14821 cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct gcgatctttg
    14881 gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc gttgaccatt
    14941 ggctcgatgg tgaacgtcat cccaggtttc agtacgacgt tggtttcacg ggagtcatag
    15001 tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccgca atattcacga
    15061 acgacggaga agccttctgc ttcgacaaat ttctgaatcg ccgcaccaat ttcgcgcaga
    15121 ttaatgcctg gttttaccat gcgtagcgcc aggtacaggc tttcttgcgt gatgcggcac
    15181 agacgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt atcgccgtgg
    15241 aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt cagcagctta
    15301 gcatcgtccg ggattccgtg gcacaccact tcattaatag aaatgcaaac ggatttcgga
    15361 taaccgtgat agccgaggca ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta
    15421 cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catatggttc gatcatctcc
    15481 agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc tggggtcttg
    15541 attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata tatatgtaag
    15601 tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg
    15661 ccactgaacg ttttctataa tagaaaattc aacgtctgat gctgtacaca gcgccaacaa
    15721 ttattggtgt ccatgacgta tttgtggtat aaagcgcgcc ggacttccga tccatttcgt
    15781 atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca cgtatcggca
    15841 catattccgg ggtgcccttt ggggtcggta atatggggta cgtggaggca taaccccaac
    15901 ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct caaggctggt
    15961 gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt catcttcggt
    16021 gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt caacgaagct
    16081 ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa tccttttcgt tggtactaaa
    16141 cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt cttcgtgaac
    16201 catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt
    16261 ctgaaagacc tggaaactca gtctcaggac ggtactttcg aaaagctgac caagaaagaa
    16321 gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac
    16381 atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca cattgctatc
    16441 aaagaagcaa acaacctggg tattccggta tttgctatcg ttgataccaa ctctgatccg
    16501 gacggtgttg acttcgttat cccgggtaac gac
//
LOCUS       DACAPB010000095        16377 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.095, whole genome shotgun sequence.
ACCESSION   DACAPB010000095 DACAPB010000000
VERSION     DACAPB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.095"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(23..220)
                     /gene="rpmI"
                     /locus_tag="IT904_002761"
     CDS             complement(23..220)
                     /gene="rpmI"
                     /locus_tag="IT904_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP3192520.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(317..859)
                     /gene="infC"
                     /locus_tag="IT904_002762"
     CDS             complement(317..859)
                     /gene="infC"
                     /locus_tag="IT904_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP3192521.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(863..2791)
                     /gene="thrS"
                     /locus_tag="IT904_002763"
     CDS             complement(863..2791)
                     /gene="thrS"
                     /locus_tag="IT904_002763"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP3192522.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3315..5214
                     /locus_tag="IT904_002764"
                     /pseudo
     CDS             3315..5214
                     /locus_tag="IT904_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            5385..5492
                     /locus_tag="IT904_002765"
     CDS             5385..5492
                     /locus_tag="IT904_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192523.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(5545..6303)
                     /locus_tag="IT904_002766"
     CDS             complement(5545..6303)
                     /locus_tag="IT904_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAP3192524.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            6590..7519
                     /gene="pfkB"
                     /locus_tag="IT904_002767"
     CDS             6590..7519
                     /gene="pfkB"
                     /locus_tag="IT904_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAP3192525.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSTLVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7620..7910
                     /gene="ghoS"
                     /locus_tag="IT904_002768"
     CDS             7620..7910
                     /gene="ghoS"
                     /locus_tag="IT904_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP3192526.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            8016..8876
                     /locus_tag="IT904_002769"
     CDS             8016..8876
                     /locus_tag="IT904_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP3192527.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSSNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(8917..9453)
                     /locus_tag="IT904_002770"
     CDS             complement(8917..9453)
                     /locus_tag="IT904_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192528.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            9600..10268
                     /gene="hxpB"
                     /locus_tag="IT904_002771"
     CDS             9600..10268
                     /gene="hxpB"
                     /locus_tag="IT904_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAP3192529.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLADVKLSSLTELTAK
                     DLLG"
     gene            10431..11021
                     /locus_tag="IT904_002772"
     CDS             10431..11021
                     /locus_tag="IT904_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP3192530.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            11154..12545
                     /locus_tag="IT904_002773"
     CDS             11154..12545
                     /locus_tag="IT904_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP3192531.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(12549..13352)
                     /locus_tag="IT904_002774"
     CDS             complement(12549..13352)
                     /locus_tag="IT904_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192532.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLQSTMTALTFFISTGQC
                     DCDEIAESNFNYMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEIRI
                     RGINKDFIGMDVTSVFRPEKMTNLISIKNRLVIHYLRTIYYEQNYIHPVGSIFAAIQK
                     NESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(13641..13904)
                     /gene="cedA"
                     /locus_tag="IT904_002775"
     CDS             complement(13641..13904)
                     /gene="cedA"
                     /locus_tag="IT904_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAP3192533.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            14087..16348
                     /gene="katE"
                     /locus_tag="IT904_002776"
     CDS             14087..16348
                     /gene="katE"
                     /locus_tag="IT904_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAP3192534.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVETDSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
BASE COUNT         4327 a         3964 c         3741 g         4345 t
ORIGIN      
        1 aaaagttaaa aacgttaacg gcttatgcgt acggcaggca cgcgattacc aggcccagat
       61 cgcctttgga aaccatggct ttcggacgca ggtgacgttt acgtttggtc gcttttttgg
      121 tcagaatgtg acgcaggtta gcgtgcttgt gcttaaaacc acctttaccg gtttttttga
      181 agcgcttagc agcaccgcgt acggtcttaa tttttggcat tttaataact tccacttcgc
      241 attgttaata aacgaaacaa aggcgaacaa agcctgtgaa gcccgaaggc tccacagaca
      301 gtgctacttg aaggccttac tgtttcttct taggagcgag caccatgatc atctggcggc
      361 cttcgatctt cgttgggaag gattcgacca ctgccagttc ttgcaaatcg tctttcacgc
      421 gattaagcac ttccataccg atttgctggt gcgccatctc acgaccgcgg aaacgcagcg
      481 tgattttggc tttatcaccc tcttcgagaa agcgaatcag gctgcggagt tttacctgat
      541 agtcgccttc atctgtacca ggacggaatt taatttcctt aacctggata actttttgct
      601 ttttcttctg ttccttagaa gacttgctct tttcatagag gaatttgccg taatccatta
      661 tacgacaaac cggcggctcg gcgttagggc tgatctcgac taagtctact ccggcttctt
      721 ctgctttctc cagagcttct ctcagactca caataccaag ctgctcgcct tccagacctg
      781 ttaagcgaac ttcctgggcg cgaatttcgc cattgatacg gttagggcgc gccgtttgaa
      841 ctcgttttcc gcctttaata ccttattcct ccaattgttt aagactgcgg ctgcgaatct
      901 cttgttgcag cttctcgatc acttcattta cgtccatgct tcccaggtct ttaccacggc
      961 gggtgcgaac ggcaactttg ccagattcca cctctttatc accacagacc agcatatatg
     1021 ggacgcgacg caaagtgtgc tcgcggattt taaagccaat cttctcattt ctcaagtctg
     1081 ctttaacacg aatgcccgca tttgatagtt tttgcgtcaa ttcgttaacg taatcagact
     1141 gtgaatcggt aatattcatg ataacaacct gaaccggcgc aagccaggtc gggaagaaac
     1201 cagcgaattc ttcggtcagg ataccgatga aacgttccat cgacccaaga attgcgcggt
     1261 gaatcattac cggtacctta cgctcgttgt cttcgcccac ataggaggcg ctcagacgag
     1321 acggcaagga gaagtccagc tgtactgtac cgcactgcca tgcacgatcg aggcagtcat
     1381 acagggtaaa ttcaattttc ggaccgtaga aagcgccttc acccagttga tattcaaacg
     1441 ggatgttgtt ttcttccagc gcaaccgcca ggtccgcctc agcacgatcc cacatttcgt
     1501 cgctgccaat acgtttttca ggacgagtgg agagtttgac gacgatcttc tcgaagccaa
     1561 aagtgctgta catatcatag actaaacgga tacatccgtt aacttcatcg cgaatttgtt
     1621 cttcagtaca gaagatatgc gcgtcatcct gggtaaatcc acgcacgcgc atcaggccat
     1681 gcagcgaacc tgacggctcg ttacggtggc agctaccaaa ctcggccata cgcagcggca
     1741 gatcgcgata agacttcagc ccctggttaa aaatttgtac gtgacccggg cagttcatcg
     1801 gcttaatgca gtattcacgg ttctcagaag acgtggtgaa cattgcatct ttgtagttgt
     1861 cccagtgacc ggttttttcc cacaggacac ggtccatcat gaacggacct ttaacttcct
     1921 gatactggta ctctttcagt ttagaacgaa caaacacttc cagttcacgg aagatggtcc
     1981 agccgtcgtt gtgccagaat accatacccg gcgcttcttc ctgcatatgg tacaggtcga
     2041 gctgtttacc gattttacgg tggtcgcgtt tcgcggcttc ttccaggcgc tgcaggtaag
     2101 cgttaagtgc ttttttgtct gcccacgccg taccgtaaat acgttgcaac attttgttgt
     2161 tgctgtcgcc acgccagtaa gcccctgccg ttttcattag tttgaaatga tggcagaaac
     2221 gcatgttcgg tacgtgcgga ccgcggcaca tatcgacata ttcttcatgg aagtacagac
     2281 ctggcttgtc atcatgggcg atgttttcgt caagaatgga gactttgtag ctctccccac
     2341 ggttggcgaa agtttcacgc gcttcgtgcc agctgacttt cttcttaatg acgtcgtagt
     2401 ttttctcagc aagctcatgc atccgcttct cgagtgcttc gacatcttcc tgggttaacg
     2461 tgcggtcaag atcaacgtcg taataaaaac cgttgtcaat aaccgggccg attgccattt
     2521 tggtatgcgg ccaaagttgt ttaatcgcgt gccctaacag gtgcgcacag gagtgacgaa
     2581 tgatctccag accgtcttcg tctttggcgg taatgatgga cagttgtgcg tcgttttcaa
     2641 tcagatcgca agcatcaacc agttcgccat taacgcgccc tgcgatacag gctttcgcca
     2701 gacctggacc aatgtccagc gcaacatcca tggggcttac agcgtggtcg taatggcgtt
     2761 ggctgccatc aggaagagtt ataacaggca ttttatatcc ttatttgcag tggtgaccca
     2821 cacgaaagat cacatacaaa gaaaaatttg tttattaaca gttcattgcg aaaccatcta
     2881 gccaacaaat gctaaattgg ttcgcaatca ggtacacaaa tgaaagacat taacctctca
     2941 ccttccaccc ggttgagcga tgttaacact aaaaaaaggg agattgtacc tttccgtttc
     3001 acattgattc gattcgcaat tcgtttgtag tgagtacacc agcaaaacgc ttaaagaaca
     3061 actgataaca tcacggtgcg tctgaaaggt ttactgcgag cggatttaca gcagcataaa
     3121 ctgaatccag caaagttcgt tgcgtgattt ggcggtagta ttttaaacgc tcatgagatt
     3181 aatgacgaag tggtcatatc acaatgataa aagcgacaca attcttataa caatttttcg
     3241 tgcacatttc gttctggcga taataattaa tcatcatcct caaaacctcc agatatatat
     3301 aaggcgaaca gattatgagt caaaacgata tcattatcag aactcattat aagtctcctc
     3361 atagcttgca catcgatagc gacataccaa caccttcatc agagcctatt aatcaatttg
     3421 cgcgccagct catcacccta cttgatacct ctgacttaag ttcgatgctg tcatactgtg
     3481 ttactcagga atttaccgca aactgtcgaa aaatatcaca aaattgttat tccactgccc
     3541 tttttaccat taactttgcc acttcaccca tccatgcaga aaatatactc attacattac
     3601 actataaaaa agaaatcatt tccttattac tggaaaccac gcctattaaa gctaaccatt
     3661 tgcgaagcat actggattat attgaacagg aacagttaac tgctgaagat cgtaaccatt
     3721 gtatgaaact gtctaaaaaa atccatagag aaaaaaacta tacacccaac agtaaatctc
     3781 aatggtagtg catgtttttt gcaatctcct tctgacgcta ttttttgtcg ccatctgtca
     3841 ttgcaatacg cccttgattc attgagaaat ggaaaaggca aagtcaacct gattaaacat
     3901 tgctcctccg ttgaatccat acagcagcat gtccccttag tccgggacgc ggagttcaga
     3961 gcattacttc gccatcctcc tgcagggagt cgcgttatcg cgagtaagga ttttggcttc
     4021 gctttagata ttttcttttg tcgaatgatg gcaaacaatg tcagtcatat gtccgcgatt
     4081 ttatatatag acaatcatac tttgtcagta aggctacgaa taaagcagtc agtgtatgga
     4141 caattaaatt atgttgtgtc cgtttacgac ccgaacgata ccaacgttgc cgtcagagac
     4201 acccacagga cagcacgggg ctttctttcg cttgataagt tcatcagttc aggtcccgat
     4261 gctcagacct gggctgatag atatgttcgc aactgtgcaa ttgctattct gcccctatta
     4321 cctgtgggag ttccaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
     4381 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
     4441 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aaccgcgcag
     4501 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
     4561 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
     4621 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
     4681 ttgtatggat tcgatgaaat aatcgatatc tttctgaatg cattaaccac tccaatagca
     4741 caagagcttt taaacaaaaa actggtgatg agtattttag ccatgaaaat acatgatggt
     4801 gagccaggat tatacgccgc aatggaaaat aatcaccctt tgtgtgtcac acggttcctc
     4861 tctaaaatta atggcatcgc ctttaaatac aagttgagca aagctaacat catggattta
     4921 ttaaaaggag ctacagcaca gggaacccct gcattataca tcgccatgag caagggtaat
     4981 gaagacgtcg tgttatctta tatatcgacg ctgggtgctt ttgcaaaaaa acattctttt
     5041 agtcaacatc agttatttac actattgacc gctaaaaatc atgacaacat gtcagctgtt
     5101 catatagcca ttcatcataa gcattataaa actgtagaaa catattatgc tgctattaat
     5161 gcaatcagcc aaagcctgag ttttagtgct gatgaaataa agacgtattt ataaccagct
     5221 atttacgata tatatcgata cataatttta ttttttcata aaacgcagaa acagtgagca
     5281 ggtgtacaac gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tgtccctttt
     5341 gttacactcc gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct
     5401 ttgataaaaa acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt
     5461 tgctccgttg ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg
     5521 cataacacgg tccggcagat aattttacat gctgtaaccc agcgataaag ttgtccgacg
     5581 atcggtatgt tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac
     5641 cttcagtcca aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt
     5701 gtcttccgcg ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca
     5761 tgcataagcc ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt
     5821 gtcgtaacgc acacctggac cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa
     5881 ctgacgacca taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt
     5941 cagccagctt gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc
     6001 gcccgccgca tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc
     6061 ccacagcgac caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga
     6121 ggagcttttc gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg
     6181 ttttttagcg ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt
     6241 cagtgacgca aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt
     6301 catttatcaa gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta
     6361 aggatcaaag attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaataactta
     6421 gcaatgtatt cttatttcat tttttgaata agcatgtggc gaaaacagat ttttatttat
     6481 atatatttat ctacaaaatt ttaaataaag ctccaataaa tcatattgtt aatttcttca
     6541 ctttccgctg attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat
     6601 ctatacgttg acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc
     6661 cgaaggaaaa ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt
     6721 cgcccgcgcc attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc
     6781 gaccggcgaa cacctggttt cactgttggc ggatgaaaat gtccccgttg ctactgtaga
     6841 agccaaagac tggacccggc agaatttaca cgtacatgtg gaagcaagcg gtgagcagta
     6901 tcgttttgtt atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca
     6961 agttctggaa attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt
     7021 gaagctggaa aaattaaccc aactgatttc cgctgcgcaa aaacaaggga tccgctgcat
     7081 catcgacagt tctggcgaag cgttaagtgc agcactggcg attggtaaca tcgagttggt
     7141 taagcctaac caaaaagaac tcagtacgct ggtgaatcgc gaactcaccc agccagacga
     7201 tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc
     7261 ccttggtcca caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc
     7321 accggtgaaa agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact
     7381 gaaactggca gaaaatgcct ctcttgaaga gatggttcgt tttggcgtag ctgcggggag
     7441 tgcagccaca ctcaatcagg gtacacgtct gtgctcccat gacgatacgc aaaaaattta
     7501 cgcttacctt tcccgctaac aaaaacattc ccccagcatt gggggaatca tcaccaacct
     7561 gtcggcaacg cgtttctccg actatgctca aaagtcatgt gataacaaag gggtgaacta
     7621 tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga
     7681 ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg
     7741 atgatgaggg aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa
     7801 gtgaagaaga aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc
     7861 ctgaaatcac catcacgacc tgggaggaat ggaatagtaa cagaaaataa atggtttttg
     7921 ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa
     7981 ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt
     8041 tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag
     8101 tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa
     8161 gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta
     8221 aaaccgtcac cgtgcctaag gtttgggcag ttggcgctga ccgtgactac agttttctgg
     8281 tgatggatta tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc
     8341 aaattgcgcg tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact
     8401 cgctctccac aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg
     8461 aacaacggat tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata
     8521 tcgacgccat cgtcgagcat attcagcagc gtctggcctc gcatcaaccg cagccttctc
     8581 tgttgcacgg cgatttatgg tccagcaact gtgcgctggg tccggatggc ccgtacattt
     8641 tcgaccctgc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata
     8701 ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt
     8761 tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg
     8821 gcggtcagca tttggttatt gctcagcagt cattggatag attattagca gcatgatatg
     8881 ggttgaggat aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa
     8941 gaatacataa cctgcagcaa tacaaataac cggcaggata tacagtgaga aaaactgcag
     9001 gaaaatagta tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc
     9061 ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc
     9121 ctggcgactc atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa
     9181 aatcacccag aacataacgt tcacgccacc attaaaattg ggcgtcgggg agttatacca
     9241 gaatagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc
     9301 gagcatgacc gcattaatgc cttcctggtt ttcctgtcgg gtattcataa atttcagcaa
     9361 agaaatcaat gtagagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa
     9421 aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt
     9481 ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg
     9541 cgcttaatca taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa
     9601 tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact
     9661 cagaaccttt atgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct
     9721 cccgtcgtaa cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt
     9781 ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg
     9841 cccgtgccat ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagccg
     9901 ttgcgttatg caaagaacaa ggtttattgg tggggctggc ctccgcgtca ccactacata
     9961 tgctggaaaa agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg
    10021 ctgaaaaact gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac
    10081 tgggcgttga cccactgacc tgcgtagcgc tggaagactc ggtaaatggc atgatcgcct
    10141 ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac
    10201 gttttgtatt agcagacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc
    10261 tcggttaatg accaggggca gtgatctcgc tgcccctggt tctttatctg aaattgcatt
    10321 caactgacgg attaatcatc aatttaagag aaagagttac accgtcacca cttccgtgca
    10381 ctgtataaaa atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg
    10441 aaggtcacct tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc
    10501 ccgtgctggc acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt
    10561 taccggacat cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac
    10621 cgatcgcccg cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc
    10681 tgctggcaac cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac
    10741 aaggaatggt gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg
    10801 ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg
    10861 gcaaccaact ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc
    10921 attcattacc cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga
    10981 tccagtttca tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt
    11041 ctggaataag caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc
    11101 caacactatg actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact
    11161 ttccattaat tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc
    11221 gccataaaca gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt
    11281 ttggccttgc cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac
    11341 agtggtttaa catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt
    11401 tagtcttcgc ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca
    11461 aaatcagttt tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg
    11521 gtgtgctggt caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag
    11581 aaactgcacg tctgaacgct attgaaagta actatgttgg taaagtctct gatctgagcg
    11641 ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca
    11701 atccgacgtc aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga
    11761 aactgctgaa ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac
    11821 aaagctgggt gatgaaactg gttcgcctgg tcatgcagtt gaccccttac ggcgttctgg
    11881 ctctaatgac caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt
    11941 tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg
    12001 gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg
    12061 cctttaccag ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc
    12121 gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc
    12181 agaacggctg cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg
    12241 gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt
    12301 ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtactgcctg
    12361 cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca
    12421 tgggccgtac ggcgttaaac gttagtggct cgatgacagc tggcacgctg accagccagt
    12481 ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc
    12541 attaattctt atgctggcaa cggtccgttt tgtatagggg ccgttgcctt actttttaaa
    12601 tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg
    12661 tccaacttat caatacattc ctggctcaat gaatgtgcct gtagcgagaa gcttaaaacc
    12721 tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca
    12781 ccacttccca ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat
    12841 attatcgaag gaaactttag gagactctca tttttttgaa tcgcagcaaa aatgctcccg
    12901 acaggatgta tataattttg ttcatagtat atagtcctta aatagtgaat aaccaacctg
    12961 ttttttatac tgatcaaatt ggtcattttt tctgggcgaa atacagaagt gacatccatt
    13021 ccaataaaat ctttatttat acctcttatc cgaatttcac taccataaag attattaatt
    13081 attactctat atttaacatt gtcctgcaga tatgtatcaa aactaagtgc tggcgctcct
    13141 ggctttgagg catcatagtt aatgttagac atataggcaa tcatataatt aaagtttgat
    13201 tctgctattt catcgcagtc acattgaccc gttgatataa agaaagtgag tgctgtcata
    13261 gtagactgca gcctttcttt aaaagagtcg aaagccaggc ttttaatatt taaatcacca
    13321 taattactct gtattaagtt tgtagaaaac atctcccgcc tcatattgtt aacaaaatta
    13381 ttatctcatt taaatctaag tcatttacaa tatgagttta agagcgacgc cacaggatga
    13441 actatcaaaa atagctcatc atgattagca aaacttaacc attttaaaat aaataaacaa
    13501 ttaaagaaaa aagatcactt atttatagca atagatcgtc aaaggcagct ttttgttaca
    13561 ggtggtttga atgaatgtag caatgaaata caaaatttca ggccatgtaa ctcccggcaa
    13621 aaccgggagg tacgtaatcc ttactcagtc acttcccctt cctggcggat ctgatttgcc
    13681 caacgctggg cggattcagg cacagtaaac gccggtgagc gcagaaatga ctctcccatc
    13741 agtacaaacg caacatattt gccacgcagc atccagacat cacgaaacga atccatcttt
    13801 atcgcatgtt ctggcggcgc gggttccgtg cgtgggacat agctaataat ctggcggttt
    13861 tgctggcgga gcggtttctt cattactggc ttcactaaac gcatattaaa aatcagaaaa
    13921 actgtagttt agccgattta gcccctgtac gtcccgcttt gcgtgtattt cataacaccg
    13981 tttccagaat agtctccgaa gcggggtctg gctggtggtc tatagttaga gagttttttg
    14041 accaaaacag cggccctttc agtaataaat taaggagacg agttcaatgt cgcaacaaaa
    14101 cgaaaagaac ccacatcagc accagtcacc actacacgat tccagcgaag cgaaaccggg
    14161 gatggactca ctggcacctg aggacggatc tcatcgtcca gcggctgaac caacaccgcc
    14221 aggtgcacaa cctaccgccc ctgggagcct gaaagcccct gatacacgta acgaaaaact
    14281 taattctctg gaagacgtac gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg
    14341 cgtgcgcatc gccgatgatc aaaactcact gcgtgccggt aaccgtggtc caacactgct
    14401 ggaagatttt attctgcgcg agaaaatcac ccactttgac catgagcgca tcccggaacg
    14461 tattgttcat gcacgcggat cagcagctca cggttatttc cagccatata aaagcttaag
    14521 cgatatcacc aaagcggatt tcctctcaga tccgaacaaa atcaccccag tatttgttag
    14581 gttctctacc gttcagggtg gcgctggctc tgccgatacc gtgcgtgata tccgtggttt
    14641 tgccaccaag ttctatactg aagagggtat ttttgacctc gttggcaata acacgccaat
    14701 cttctttatc caggatgcgc ataaattccc cgattttgtt catgcggtaa aaccagaacc
    14761 gcactgggca attccacaag ggcaaagtgc ccacgatact ttctgggatt atgtttctct
    14821 gcaacctgaa actctgcaca acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag
    14881 ttaccgcacc atggaaggct tcggtattca caccttccgc ctgattaatg ccgaagggaa
    14941 ggcaacgttt gtacgtttcc actggaaacc actggcaggt aaagcctcac tcgtttggga
    15001 tgaagcacaa aaactcaccg gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc
    15061 cattgaagca ggcgattttc cggaatacga actgggcttc cagttgattc ctgaagaaga
    15121 tgaattcaag ttcgacttcg atcttctcga tccaaccaaa cttatcccgg aagaactggt
    15181 gcccgttcag cgtgtcggca aaatggtgct caatcgcaac ccggataact tctttgccga
    15241 aaacgaacaa gcagcattcc atccaggtca tattgttcct ggtctggact tcaccaacga
    15301 tccgctgttg caggggcgtt tattctccta taccgataca caaatcagtc gtcttggcgg
    15361 gccaaatttc catgagattc cgattaaccg tccgacctgc ccttaccata atttccagcg
    15421 tgacggcatg catcgcatgg ggatcgacac taacccggct aattacgaac cgaactcgat
    15481 taacgataac tggccgcgcg aaacaccgcc ggggccgaaa cgcggcggtt ttgaatcata
    15541 ccaggagcgc gtggaaggca ataaagttcg cgagcgcagc ccatcgtttg gcgaatatta
    15601 ttcccatccg cgtctgttct ggctaagtca gacgccattt gagcagcgcc atattgtcga
    15661 tggtttcagt tttgagttaa gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga
    15721 ccagctggcg catattgatc tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga
    15781 actgactgac gaccagctga atatcacccc acctccggac gtcaacggtc tgaaaaagga
    15841 tccatcctta agtttgtacg ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat
    15901 tttacttaat gatgaagtga gatcggcaga ccttctggcc attctcaagg cgctgaaggc
    15961 caaaggcgtt catgccaaac tgctctactc ccgaatgggt gaagtgactg cggatgacgg
    16021 tacggtgttg cctatagccg ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt
    16081 cattgtccct tgcggcaata tcgcggatat cgctgacaac ggcgatgcca actactacct
    16141 gatggaagcc tacaaacacc ttaaaccgat tgcgctggcg ggtgacgcgc gcaagtttaa
    16201 agcaacaatc aaggtcgctg accagggtga agaagggatt gtggaaactg acagcgctga
    16261 cggtagtttt atggatgaac tgctaacgct gatggcagca caccgcgtgt ggtcacgcat
    16321 tcctaagatt gacaaaattc ctgcctgatg ggaggcggcg caattgcgcc gcctcaa
//
LOCUS       DACAPB010000096        16352 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.096, whole genome shotgun sequence.
ACCESSION   DACAPB010000096 DACAPB010000000
VERSION     DACAPB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.096"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1250)
                     /gene="treB"
                     /locus_tag="IT904_002777"
     CDS             complement(<1..1250)
                     /gene="treB"
                     /locus_tag="IT904_002777"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAP3192535.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNG"
     gene            complement(1369..2316)
                     /gene="treR"
                     /locus_tag="IT904_002778"
     CDS             complement(1369..2316)
                     /gene="treR"
                     /locus_tag="IT904_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAP3192536.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            2501..2554
                     /gene="mgtL"
                     /locus_tag="IT904_002779"
     CDS             2501..2554
                     /gene="mgtL"
                     /locus_tag="IT904_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAP3192537.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            2695..5391
                     /gene="mgtA"
                     /locus_tag="IT904_002780"
     CDS             2695..5391
                     /gene="mgtA"
                     /locus_tag="IT904_002780"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP3192538.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWMEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(5597..5983)
                     /gene="ridA"
                     /locus_tag="IT904_002781"
     CDS             complement(5597..5983)
                     /gene="ridA"
                     /locus_tag="IT904_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAP3192539.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6056..6517)
                     /gene="pyrI"
                     /locus_tag="IT904_002782"
     CDS             complement(6056..6517)
                     /gene="pyrI"
                     /locus_tag="IT904_002782"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAP3192540.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(6530..7465)
                     /gene="pyrB"
                     /locus_tag="IT904_002783"
     CDS             complement(6530..7465)
                     /gene="pyrB"
                     /locus_tag="IT904_002783"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAP3192541.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(7469..7603)
                     /gene="pyrL"
                     /locus_tag="IT904_002784"
     CDS             complement(7469..7603)
                     /gene="pyrL"
                     /locus_tag="IT904_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAP3192542.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(7884..8279)
                     /locus_tag="IT904_002785"
     CDS             complement(7884..8279)
                     /locus_tag="IT904_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP3192543.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(8410..9123)
                     /gene="bdcA"
                     /locus_tag="IT904_002786"
     CDS             complement(8410..9123)
                     /gene="bdcA"
                     /locus_tag="IT904_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3192544.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            9194..9787
                     /locus_tag="IT904_002787"
     CDS             9194..9787
                     /locus_tag="IT904_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3192545.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            9932..10384
                     /locus_tag="IT904_002788"
     CDS             9932..10384
                     /locus_tag="IT904_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP3192546.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            10507..12101
                     /locus_tag="IT904_002789"
                     /pseudo
     CDS             10507..12101
                     /locus_tag="IT904_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(12158..13162)
                     /gene="argF"
                     /locus_tag="IT904_002790"
     CDS             complement(12158..13162)
                     /gene="argF"
                     /locus_tag="IT904_002790"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP3192547.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPETALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            13324..13740
                     /gene="rraB"
                     /locus_tag="IT904_002791"
     CDS             13324..13740
                     /gene="rraB"
                     /locus_tag="IT904_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAP3192548.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(13786..14289)
                     /locus_tag="IT904_002792"
     CDS             complement(13786..14289)
                     /locus_tag="IT904_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP3192549.1"
                     /translation="MNNIAPQSSVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            14482..15678
                     /locus_tag="IT904_002793"
     CDS             14482..15678
                     /locus_tag="IT904_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAP3192550.1"
                     /translation="MAQVINKMDVPPHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYYSSVYANDDIENLQQFMEVQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            complement(15734..>16352)
                     /locus_tag="IT904_002794"
     CDS             complement(15734..>16352)
                     /locus_tag="IT904_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HAP3192551.1"
                     /translation="LRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVD
                     EAALADTEWLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLA
                     RLESITVLPADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRI
                     ENKLANEGFVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
BASE COUNT         4152 a         3783 c         4067 g         4350 t
ORIGIN      
        1 ccgttcaggc cgcatagcaa tcctgccaga ccagaaccaa tcatcgcgca cagcatcggg
       61 aagcgatatt tcaggttgat gccgtacatt gcaggctcag tgaccccaag ccaggcggag
      121 atagcggcag gcacggagat ctcgcgttca ttgtgcttgc ggctggaaat gataatgcct
      181 atcacggcgg agccctgagc gatattcgac agcgcaatca gcggccacac tggcgtgcca
      241 cccatgcttt gaatcatctg caaatcaata gcaagggtgg tctggtgtac gccggtgatc
      301 accagcggcg cgtacaggaa gccaaacaat gccgcaccaa tcggagcaaa gctgccggtc
      361 atcaggtgac gtaccgcaaa ggcaacgcca tcgccaatca tgcgaccaaa cggaccaatc
      421 agcgcatggg cgaggaacac cgcgaggatc agcgaacaga cgggtaccac caccagatag
      481 aggtaatccg gcacgatgcg tttaaggcga gtttcaataa cgcccagcgc cagtccggct
      541 aacagtgccg ggatcacctg cgcctgatag cctactttgg cgatgctgaa catgccaaag
      601 tcccacactt ccggcagctg ctgcccgagc agataagcgt tcatcagctg tggagaaacc
      661 agtgtcacgc caagcacgat accgaggatc ggcgtgccgc ccattttttt caccgctgac
      721 cagcaaatcc cgaccggcag gtagaagaag atcgcttcac cgatcaacca cagaaaatcg
      781 tagatcgttt gcagggaagg gtacatttgc gccagcgttt gaccgttgct catgggcaaa
      841 tcgccgatca cattgcgaaa accgaggatc aaaccgccgc taatcaacgc gggcagcaac
      901 gggaagaaga tctccgcgaa atgagagatc aactgctcat gccatttcat attctgccgg
      961 gcagcttttt ttacctgctc tttatcaacc tgcgcctgtc cggttgacgc aatcagtgct
     1021 tgatagtaat cacccacatt ggtgccaatc accacctgaa attgcccggc attggtgaaa
     1081 cagcctttca ccatggggag ttgctcaatt tctttcggtc tggcattggc cggttggttg
     1141 aggacaaagc gcaggcgagt aatacagtgg ctcaccgtcg caatattgcc gcgcccgccg
     1201 accagttcaa tcaaccgatc gatatccgtt tggtttattt tgctcatcat aaagccccat
     1261 ggcagatgac atttttggtt ggctgcagaa tatattgcgc ggaaaaattt aaaaacggga
     1321 acgttcccga aacgcagcga agatcacaat ttatcgttca ggaaacgatc aggacagggt
     1381 ggcggggatg atgatttgtt gcggttcgct gcgcccggtt acctgtgcga tcaattggca
     1441 ggccgcctgg cgtccagctt cggcgtaacc gggatctacg gtgacgatct ccggatggag
     1501 gaatttcatt aacggtgtat taccgacgct cgccagttgc aaggtgtcga tgcgttgctc
     1561 ttgcaggtat ttacttgcgc caagtgccag cgtgtcggtt gcgcacagta acgcagtagt
     1621 ttcaggcgta atcacttttg ccacgttctc atagccttgc ttcatagcaa gccctggcag
     1681 agcggcaacg ggatgcagtt tatgcgcttt gcagaacgcc aggtaggctt cgtgacgtcg
     1741 cttaccggtt gtcacgtcac tatgcggcac gccgagataa ctgatattac gatgcccctg
     1801 gtcatacagc cgttgcatca ggatcttgat tgccccttcg tcgtcataac agaccgaagc
     1861 aaagcctttt gcgtcacgcg ccagcagaac cagcgatgac tgccagtggg ctaacatttc
     1921 ttctgttatg ccagtaaaac cgaacagcac tacgccgtcg atattacgcc gtttcagcac
     1981 ccccaaatgt tcggcaacta attgtgggga aaactgactt tccatcatga ttgggtcgta
     2041 accttgttca tagaacgctg gcagcatggt ttgaacggcg agattttctg acaacgaatc
     2101 cagacgggta acaatgatgg cgaccacttt atcgctttgc ccacgcatag cgcgcgcaga
     2161 gcgggaaggg gaaaatccat gctgattcat cactgcttca acacgctcgc gggtgcgctg
     2221 gctcacgccg ctttcgttat tcagcacccg ggaaactgta gatttcccca cgccgcttaa
     2281 gcgtgcgatg tctttgatgg tcagccgatt ttgcatcctg ttgtcctgta acgtgttgtt
     2341 taattatttg agcctaactt tacccgtgca ttcagcaatg ggtaaagtct ggtttatcgt
     2401 tggtttagtt gccagcaggt attatatcgc catagatgct acgaatatta ttggattctc
     2461 cttattattt gcggcgcttt tttcacttac cggaggttat atggaacctg atcccacgcc
     2521 tctccctcga cggagattaa aacttttccg gtaagcccgt cttttcacgg cgttaccgga
     2581 tgcgtaaggc cgtgacgttt taacgtccct gctcagcttt attaccttca ggtaaggctt
     2641 cgccacgcct gaagacattt ctgtactgtt tcagacagtg cggagggact ccttatgttt
     2701 aaagaaattt ttacccggct cattcgccat ttaccttccc gtctggttca tcgcgatcca
     2761 ttgcctggcg cgcagcagac agtgaatacg gcggtcccgc cgtccttaag tgcgcattgc
     2821 ctgaaaatgg cggtgatgcc cgaagaagaa ttatggaaaa cgttcgacac ccatccggaa
     2881 gggttaaatc aggcggaagt ggaatctgcc cgcgaacaac atggtgaaaa taaattaccc
     2941 gcacaacaac cgtcgccgtg gtgggtacat ttatgggtct gctatcgcaa cccatttaat
     3001 attttactca ccattctcgg cgctatttct tacgccacgg aagatttatt tgccgcgggc
     3061 gttatcgcgc taatggtcgc tatttctacg ttgctgaact ttattcagga agcacgttcc
     3121 actaaagcgg cagatgccct gaaagcgatg gtcagcaata ctgcgacggt gctgcgcgta
     3181 attaacgaca aaggcgaaaa tggctggatg gagatcccga tcgaccagct ggtgcccggc
     3241 gatattataa aactggcggc gggggatatg atcccggcag atttacgtat cttgcaggcg
     3301 cgggatctgt tcgtcgctca ggcgtcgtta accggtgagt ctctgcccgt agaaaaagca
     3361 gctaccactc gccagccgga gcacagcaat ccgctggagt gcgacaccct gtgttttatg
     3421 ggcaccaccg tggtgagcgg cacggcacaa gcaatggtga ttgctacagg tgccaatacc
     3481 tggtttggtc aactggcggg gcgtgttagt gagcaggaaa gcgagccgaa tgcctttcag
     3541 caagggatca gccgcgtcag tatgctgctg attcgcttta tgctggtgat ggctccggtg
     3601 gtgctgttaa tcaatggtta caccaaaggc gactggtggg aagcggcact gtttgcgctt
     3661 tcggtcgcgg taggcttaac gccggaaatg ttgccgatga ttgttacctc gacgctggcg
     3721 cgcggcgcag taaagctgtc gaaacagaaa gtgatcgtca aacatctgga tgctattcag
     3781 aactttggcg caatggatat tctgtgcact gataaaactg gcaccctgac acaggacaaa
     3841 attgtgctgg agaatcatac cgatatctcc ggtaaaacca gcgaacgcgt gctgcatagc
     3901 gcgtggttga acagtcatta tcagaccgga cttaaaaacc tgctcgatac agcggtgctc
     3961 gaaggtacgg atgaagagtc agcgcgctcg ctggccagtc gttggcaaaa aatcgatgag
     4021 attccgtttg atttcgagcg tcgccggatg tcggtggtag tggcagaaaa taccgagcac
     4081 catcagctgg tttgcaaagg tgcattgcag gaaatcctca atgtgtgttc gcaggtgcgt
     4141 cacaatggcg agattgtgcc gctcgatgac atcatgctgc gtaagattaa gcgggttact
     4201 gatacgctga atcgtcaggg gctgcgcgtg gttgcggtgg cgacgaaata cctgccagcg
     4261 cgtgaaggag attaccagcg ggcggatgaa tccgacctga tcctcgaagg atatattgct
     4321 ttccttgatc cgccaaaaga gacaactgct ccggcactga aggcattaaa agcgagtgga
     4381 attaccgtaa aaatccttac tggcgacagt gagttagttg cagcgaaagt gtgccatgaa
     4441 gtggggctgg acgcgggaga ggtggtcatt ggtagtgata ttgaaacgct atctgacgac
     4501 gaactggcaa atctcgcaca gcgcaccacg ctgtttgctc gtctgacgcc gatgcataaa
     4561 gaacgcattg tgaccttact gaagcgcgaa gggcatgtgg ttggctttat gggcgatggt
     4621 attaatgatg cgcccgcctt acgcgctgcg gatatcggca tttctgtgga cggcgcggta
     4681 gatattgccc gtgaagcggc tgatatcatc ctgctggaaa aaagcctgat ggtgctggaa
     4741 gagggggtta ttgaaggacg tcgcactttc gccaacatgc tgaaatacat caaaatgacg
     4801 gcgagctcca acttcggtaa tgtgttcagc gtgctggtgg cgagtgcttt cttgcccttc
     4861 ctgccgatgt tgccgttaca cttgcttatt cagaacctgc tgtatgatgt gtcacaggtg
     4921 gcgatcccgt ttgataacgt cgacgacgag caaattcaaa agccgcagcg ttggaacccg
     4981 gcggatctgg ggcgctttat gatcttcttc ggaccgatca gctcgatctt cgatattctg
     5041 acgttttgcc tgatgtggtg ggtattccat gccaacacgc cggaaacgca aacgctgttc
     5101 cagtcgggat ggtttgtggt gggcttactg tcgcaaacgc tgattgtgca tatgatccgc
     5161 acccgccgtg tgccgtttat tcagagctgt gcatcgtggc cgttaatgat catgaccgtg
     5221 atcgtgatga ttgtcgggat cgcattgccg ttttcaccgc tggccagtta tctgcaatta
     5281 caggcgctgc cgttaagcta tttcccgtgg ctggttgcga ttctggcagg gtatatgaca
     5341 ttaacccagt tggtgaaagg gttctatagc cgtcgttacg gctggcaata aagaataaac
     5401 tgggcacgat agccccgatt cagattgcag acaaagtaag ctttgttcat gccggatgcg
     5461 gcgtaaatgc cttatccggc caacaaattc aataaattgc aggaatcacg taggcctgat
     5521 aagcgtagcg catcaggcga ttttgcgttt gtcatcagac ttaatccggg catgatagca
     5581 cggatttcca tcaagattag cgacgaacag cgatcgcttc gatctcaatc ttcacgtctt
     5641 tcggcagacg ggcgacttca acgcaagaac gtgccgggaa ggtggcgttg tgttcggtga
     5701 agaaggcttc gtaggtagca ttaacggtag cgaagtcgtt cagatctttc acaaacacgg
     5761 tggttttaac gatgtcgccc actttcaggc cagcggcttc gacgatcgct tttacgttat
     5821 ccagcgactg acgtgcctgt gcagcgacgt ctgccggtac ttcacccgtt ttcggattta
     5881 ccgggatctg accggaggtg atgatcatat tgcccagatc aacgccctgt acgtaaggac
     5941 cgatagctgc cggtgcattt tccgtcgcga tagttttgct catgatttct cctttattac
     6001 agcggtaaaa agtctggctc attataggga gccagacttt tattaccaac cgcaattaat
     6061 tggccagcac cacattatgg gaaaactctt tttcacagta tttgcatttg agcgcgatgt
     6121 cattggcgcg ttttcgcacg gcaaagctgg aagaaaccgg ttcggcatgg ctgatacagt
     6181 tgctgttcgg gcagaccagc acattgtcga tgcgctctgg cagactgggg cgcgatttac
     6241 ccaccacttc atagttgtcg atacggttaa ccgtggcttg cggcgcatac aatgccagtt
     6301 gatctacctg atcttcactc aaaaaggtat tttcgatttt gatcagatct ttgcggccca
     6361 tctcgccaga aggcaagttc agaccgatgg tgatgcgctg atccgtttcg gtcagcttga
     6421 acagactcaa cagcttaaaa ccgatctggg cggggatatg gtcaattacc gtgccgcgtt
     6481 taatagcttc aacctgcaat ttattatcgt gtgtcatctc tatttcccct tacagtacca
     6541 gatcgcgatt cagaaccagt gccagtaacg cctggcgagc gaaaatcccg ttgcctgcct
     6601 gctggaagta ccaggcgtgt ggcgttttat ccacatcggt ggcaatctca tcaacacgcg
     6661 gcagcggatg cagcactttc atattggctt tggcgttgtg gagatcgctg gcgcgaagaa
     6721 caaactgcgc tttcacgttg gcgtactcgg acgggtccag acgctctttt tgcacgcggg
     6781 tcatgtacag gatgtctact tccgccatca cttcttcaat agagctgtgc agactccatg
     6841 cgatcccttt ttcatcgagc atatccagaa tgtattgcgg cattgccagc gcgtccggcg
     6901 cgatgaagta aaaacggttg ccgtcgaact tcgctaacgc ctgagtcagg gagtgaacgg
     6961 tgcggccata tttcaggtca ccaaccattg cgacgtggag attgtccaga cgcccctggg
     7021 tttcctgaat agtgaataag tccagcaagg tttgcgtcgg atgttggttg gagccatcac
     7081 cggcattcag taccggtaca ttgccggaaa actcggtggc caggcgcgcc gcaccttcct
     7141 gcggatgacg catcactatc gcatcgacgt aagtgctgat aaccgaaatg gtatcggcca
     7201 gcgtttcgcc ctttttaccc agtgatgtat tggcgctgtc ggagaagccc accacgctgg
     7261 cacccaggcg gtgcatagat gtttcgaaag agaggcgggt acgggtagag gcttcgaaga
     7321 aacagctggc aatgactttg tgcttcaaca gctctggttg cgggtttgct ttcagtttcg
     7381 ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag gtcgtttatg gaaatgatat
     7441 gtttctgata tagcggatta gccatctttt atctcctgac gcctgggcaa aaaaaagccc
     7501 ctcgattgag gggctgggaa tgggtgatca acgggaagaa aaacggcagg ccagcgtctt
     7561 ttttcagacg cggtaagaca aaatgtcgaa cacactgaac catacatcct cccggcaaat
     7621 tgtccggcat tatactcata gccagaagcg gtgcaagcat ttgatgcaat attttgtcag
     7681 cgcaaacggt ttatttgaat taaaagtcaa aatgtatgca tttttatttg ctttgattct
     7741 ggaagagaac tgtcgttcgg tatcaataat tgcagacatt tcctgctttt ttctttttca
     7801 ctttcacgca atcagatatg cattttattc cattcatccg acttatacgg cggagtgtga
     7861 aaacgaacgg ctaacactat tgcttacagc tcagggatgc gcgctatcac tttaatttca
     7921 aaatcaaagc ctgccagcca ggtaacgccc accgccgtcc agtttggata aggaggggcg
     7981 ctaaatattt catttttgac cgtcatgatg tcttcaaatt gtttttccgg atcggtatga
     8041 aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg cagctgccag ggtcgcatgc
     8101 aaattatcaa atgccagtct gacttgttgc tgaaaatcgg gttctggtgt tccgtcctct
     8161 cgacttccta cttgcccgga aacaaacagc aaatcgccgg aacgaatagc cgccgaataa
     8221 cgatgttcag catacagtga atgtcggcca gcagggaaaa cagcggttct ttctaccatt
     8281 tggttatcct caagatttac gacatgaaca gaagatttat atttaccggg agccgctttt
     8341 agggaacgac gtgagtaaac aaaacccaga catcatggat aatggctggg cttaattgag
     8401 cgtagtcggt tatgccccaa acgcgccatc aatggtatgc atcgcgccgg taacaaaact
     8461 ggcttctggc cctgctaacc atgcgaccat tccagcgacc tcttccggtt gcccatgtct
     8521 tttgatagcc ataaaactat gcaacatatc gcgcattggc ccgttggcgg gattagcgtc
     8581 ggtatctatt ggccctggct ggacgacgtt gatggtgatc ccacgcggtc caaaatcacg
     8641 ggccagcccg cgcgccatgc cttgcagggc agatttactg gcggcataag cagccatgcc
     8701 tgcaacaggc atacgatcgc cattcacgga gccgatgatt aagatgcgcc cgccttcggg
     8761 catctgccgg gcggcttcaa cagaggcatg gtaaggggca tgaatattga ttttgaaaag
     8821 gcgatcaata tcgtcggcat ttaattccag ggcatcgcca aagacgccaa tacctgcatt
     8881 taccaccagg atatccaatg cgccgctctt acgaacgaca tcaataacag cgtctctgtc
     8941 agcactatct gtgaatactg ctgtcgctcc agtctcatgt gccaggcgtt cagcggcatc
     9001 tttcgacccc gcataggtga atcgtacatt ggctccatcg gtgacgaaac gacgtacgat
     9061 agcggcacct attccacggc taccaccgag gatgagaact gtcttacctg taaaagcgcc
     9121 cataaggact ccttgattta ttatgtaaca tgcattacaa aactgtttta actttctgtc
     9181 aacaagtttt gtaatggtca ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt
     9241 cgctcctgag caggcagtct ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc
     9301 tgttagtgtt gctgaagtta ctgattatct tggtattaac cccccgagcc tctacgcggc
     9361 ttttggcaat aaggctgggt tatttagccg tgtactcaat gaatatgtcg gtacggaagc
     9421 tattccgctt gccgatattc ttcgtgatga tcgtccggta ggggagtgcc tggctgaggt
     9481 attaaaagaa gcggcgcgca gatatagcca aaacggcggc tgcgctggct gtatggttct
     9541 tgaaggcatt catagtcatg atccacaagc gcgtgatatt gccgttcaat attatcacgc
     9601 cgcagaaacg accatttatg actatatcgc caggcggcat ccacaaagcg cacaatgtgt
     9661 gactgatttt atgagtaccg tgatgtcagg gctttctgcg aaggcacgag aggggcactc
     9721 cctcgaacaa ctctgtgcaa cagctgcact ggcgggggaa gcgataaaaa ctattctcaa
     9781 ggagtgatgc tgaccttgat ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag
     9841 acaataattc acgcgttaag gctggcggaa ttgatcatct tttcgtataa cggtggaaat
     9901 gaaacattgt tttaattaag gagtggaaaa gatgatcatc ggaaatattc ataatcttca
     9961 gccgtggcta ccccaggagt tacgccaggc gattgagcat atcaaagcac acgttacggc
    10021 agaaacgcca aagggcaagc acgatatcga aggtaatcgt ctgttttatc ttatctcgga
    10081 agatatgacc gagccgtacg aagctcgccg tgcggagtac catgcccgct atctcgacat
    10141 tcagattgtg ttaaaaggtc aggaaggcat gaccttcagc acgcaacctg caggcacgcc
    10201 ggataccgac tggttagctg ataaagacat cgcatttttg ccggaaggcg ttgatgagaa
    10261 aacagttatc ctaaatgaag gtgattttgt tgtgttttat ccgggggaag tgcataaacc
    10321 gctgtgcgca gtgggcgcgc cagcccgggt tcgcaaagca gtagtgaaga tgctgatggc
    10381 gtgatgactt ttcgccgtaa ataactccag gtttacggcg agcttatgaa aagagcgttt
    10441 tttgatattt ttttgtgagt agaatttgta atgcttagac gttcttatta actcaaggag
    10501 tccgtcatga gcaaaatatc agatttgaat tattctcaac acattacatt agccgacaat
    10561 tttaaacaaa aaagtgaagt tttaaatacc tggcgtgttg gaatgaatga ttttgcccgt
    10621 attgccgggg gcaggatagc agaaggaata ttctttctcc cagatcattt ttggagtttt
    10681 tggctaagat atttaccctg ggttatgtgg attttagcaa acgctccaac gaagcgggta
    10741 gaaatatgat ggctcatatt aagtcctcat cttatatcaa aaataatgat ggtagtgaga
    10801 taatgaagtt tgttatgaat aatcctgaag gggagcgagc ggatttatct aaggtggaaa
    10861 tagaaattac actttctact accactacta tggggactcg tcaaggacat acagccatta
    10921 tatttccaca atctgatttt ttgagttttc gttatgaagg taagtctttt gaaagaaaag
    10981 atgatagttc attacacctg attactaaca aggttctggc gtgttaccaa cgtgaagcta
    11041 acaagaatat ggcgcttcta ttaaataatc atcagttgaa ctggaaggac gcaattgatt
    11101 ataacgagtt attagaaaag ccattagcca gtacattaga aaaaataaag aaagaacatc
    11161 tgctattaat gcctcatgta tgtgatgaca cgattccata cttactaggc gaaggtggta
    11221 tacttgaaga aataaatgat ctgaaaacgt tacatgatac cagaattgct agtggtacag
    11281 aaggtaatga tgaaattaat aacatcaaaa ttaacctgtc taatattctt attgattcct
    11341 tagatgatgc aaaagttaat ctcgaaaagg tcatcgattc gatgttgaag acttttttaa
    11401 aattgccata tattaatgat gtaaaaattc tggagtggtg ttttaatcaa agtatgcaat
    11461 attctgatga tactacaaag ataaagcatg cccgctccgt gatcgatcat attgatttta
    11521 actgcgatca gtctaaaata gctgagacat tatttttcaa cctcaataaa gaaccctata
    11581 aaaacagccc tgagttacag gaattgattt gggagaagtt ggttgtatat gtcaatgatt
    11641 ttaatttaag taatcaagaa aaatcgcgct taatactaag gctatttgat gatgttaaat
    11701 tggtttttga taaagtgcct ctcagtattt tagtgaatga tatttttttg aaggattttt
    11761 tcatgaagca acctgatttt gctaagtggt acttctatca attacttaag agttatgagg
    11821 gagaacagat gtatcttaat gagttaggat atgtttatgg tgatgaagaa aaaacaaaag
    11881 aaattgtaaa taaacttccg ggttatgtcg ttaagatttt tgaagaaaag atcgataatg
    11941 aattaaaaat tagaacaaga aagatggaaa ccctgcggga tgggaaaatc aatatatatg
    12001 attatattaa tgaaaaacag ttagaaaaat taaatccacc gcaagattta cgttctgcaa
    12061 tagaaaaaat tgggtggaag aatagaccta tcactgcata aaataatgtg atgccgggat
    12121 agttttgtat ttcccggcat ctttatagcg atatcaatta tttactgagc gtcgcgacca
    12181 tcaccgcttt gatggtatgc atgcggtttt ccgcctgatc aaatacaatg ctggcggcag
    12241 attcgaagac ctcatcagtc acttccattc cgccatgtag gccaaactct tccgccattt
    12301 tcttgccaag cgtcgtttgg tcgtcatgaa acgcgggcag gcagtggagg aatttgacct
    12361 ccgggttgcc ggtcaactgc atcatcttgc tgttcacctg ataatcacgc agtaatgcaa
    12421 tgcgttctgc ccatttctct tttgcttccc ccatcgacac ccacacatcg gtatagataa
    12481 agtcagcacc ttcaactcct ttggctacat cttccgttag cgtaatattc ccgccatttt
    12541 gctgtgccag agcgcggcat tccgtaacca gcgcagtttc cggccagcat gcctgtggcg
    12601 cgaccagacg caaatccaga ccggtaagcg ccgcagcttc gagcatcgaa ttgcccatgt
    12661 tgttacgtgc gtcacctgca tagaccagcg tcatttcgtt gaacgctttg ccgggtaaat
    12721 gctcctgcat ggtgagaaga tccgccagca gctgcgtggg gtgaaactca ttggtcaggc
    12781 cgttccatac cggcacgcca gcgtattccg ccagtgtttc gacaatctcc tgaccatagc
    12841 cgcgatactg aataccgtca tacatgcgac caagcacgcg ggcagtgtct ttaatcgact
    12901 ctttatgacc aatctggctg ccgcttgggc cgagataagt aacgcgagca ccttggtcat
    12961 atgcggcaac ttcgaaagag catcgggtac gggtcgagtc tttttcgaag atgagcgcga
    13021 tgtttttacc agtgagtttg gcttcttctt taccgctttt cttatcggct ttcagcttcg
    13081 cggctaactg caacaggctg ttgagttcag ctggcgtgaa atcgagtaat ttcaggaaat
    13141 gcttatgata aaacccggac atagatccct cctgtggcta tcgcctcaat gaattaaaat
    13201 tcaatttata tggatgatta ttcatttgca agtctaaagc ataaatcttt gtcacaaagg
    13261 tggaggcaat gtcagtggtg tgtgacaata agagtatcgg caggacatta agaggaatga
    13321 gccatggcaa acccggaaca actggaagaa cagcgtgaag aaacacgttt gattattgaa
    13381 gaattactgg aagatggcag cgatccggac gcgctgtaca ccatcgaaca ccatctttcc
    13441 gcagacgatc tggaaaccct ggaaaaagca gcagttgaag cgtttaaact cggttacgaa
    13501 gtgaccgatc cagaagagct ggaagttgaa gatggtgaca tcgtgatttg ctgcgacatc
    13561 ctcagcgagt gcgcgttgaa tgccgatctg atcgatgccc aggttgaaca actgatgacg
    13621 ctggcggaga aatttgacgt tgagtacgat ggttggggca cttactttga agatcctaac
    13681 ggcgaagacg gcgacgatga agattttgtc gacgaagacg atgacggggt tcgccactaa
    13741 ttaattgatt aaaatgcccg atagcacaca ctagcgggca tttttttaga gttcgcgtag
    13801 catccgcact tcacaatcga catggcccgt gcagccaagc gcatagtcga tatgctcaaa
    13861 gcccaaatgc tcataaagcg caatggcttc ctttaaaaaa gcggtcgttt ccagatagca
    13921 gcgtttgaag cccatctctc gtgcctgctc catcgccatt aaggccagtt ttttcgccag
    13981 ccctttaccg cgaatagccg gaagaaaata catcttttgc agttcgcaaa tgtccgattc
    14041 actacctgct aatggcgcaa tcccaccgcc gccgaccact tcaccatcga actcaaccac
    14101 ccaatatgca tggccaggct gactatacac ttgatacaac tcgtcaaggt tcggatcggc
    14161 gacggtgtag cctttatcag cggtaagacc gtattcggcg gatacctgac gaatgacgcg
    14221 ggcgatagca ggattatcct gcaacgtcag gcgacgcatt acagatgatt gcggcgcaat
    14281 gttattcata gtttaaccca ataaaaataa agtaattata catatgttta ataccactct
    14341 cttcttacgt gcgcaagcga cttataaagt aataaagttt ttgccagcct attactgata
    14401 agagtattgt aacgtggcag aacattttac tgtctgtatc tataaattat atcaatggat
    14461 tttttaattc agggaatttt tatggctcaa gttattaata aaatggatgt tccgccccat
    14521 tcgtttgttt ttcatggtac aggtgagaga tattttctta tttgtgtggt gaatgtgttg
    14581 ttgacgatta taacgctagg tatctattta ccatgggcat taatgaaatg taagcgttat
    14641 ctctatgcta atatggaagt taacggacaa cgattttctt atggaattac tggtgggaat
    14701 gtttttgtta gttgtcttgt ttttgttttt ttctatttcg caatcttaat gacagtgtca
    14761 gcagatatgc cgcttgttgg ctgtgttttg actttgtcac tgttggtttt gcttatattt
    14821 atggcagcaa aaggactgcg ttatcaggcc ttgatgacca gtctcaacgg cgtaagattt
    14881 agttttaatt gctctatgaa agggttctgg tgggtaacct ttttcttgcc gattttaatg
    14941 gccattggga tggggactgt tttctttatc tcgacaaaga tgctacatgc caatagttca
    15001 agtagtgtta ttatatctgt ggttctgatg gcaatagttg gtattgtttc cattggtatt
    15061 tttaatggta ctttatatag cctggtaatg agttttctct ggagcaatac cagtttcggt
    15121 atacatcgtt tcaaggtgaa attagatact gcgtattgta taaaatatgc catcctcgca
    15181 tttttagctt tattaccttt tctcgctgtt gctggttata ttatcttcga tcaaatatta
    15241 aatgagtatt atagttctgt gtatgcaaat gatgatattg agaatttaca gcaatttatg
    15301 gaagtgcaac gtaaaatgat aatcgcgcag ttaatctatt attttgggat tgctgttagc
    15361 accagttatt taacggtgtc gttgcgaaat cattttatga gcaacctgtc actgaatgat
    15421 gggcgtattc gttttcgctc aactttaaca taccacggta tgctttatcg catgtgtgcg
    15481 ttggtggtga tatccgggat tacgggcggt ctggcttatc cactgctgaa aatatggatg
    15541 attgactggc aggcaaaaaa tacatatttg ctgggcgatt tggatgacct tcctttaatc
    15601 aataaagaag aacaaccaga taaaggcttc ttagccagta tttcacgggg aattatgcct
    15661 tctttaccat ttctgtaaga gataaaaaag gccggagtat gctccggcct tcgttttcat
    15721 cactgtgttt tgattacagc gcggcgataa cagcctgctg ttcaatcagc ttcgctttcg
    15781 cttctgcata gccttccagc ttctcacgct ctttcgcgat gaccgcttcc ggtgcgcggg
    15841 cgacaaagcc ttcattcgcc agcttgttct cgatacggct gatttcacct tcaatcttcg
    15901 ccacttcttt cgccagacgc gccagctcat cttctttgtt gatgaggcca gccatcggga
    15961 tcagcagctc tgcaccgtcg atgatcttcg taacggaaac cggacctttg tcatcggcag
    16021 gcagcacggt gatgctttcc agacgcgcca gggtttgcag gaagccacgg ttttcattta
    16081 cgcgacgttc tgcatccgcg ctgcaaccac gcagcagcag ctccagcggt ttgcccggcg
    16141 cgatgttcat ttctgcacgg atgttacgta ccgcaacgat cgcctgcttc aaccactctg
    16201 tgtcggccag tgcggcttca tcaacctgag atgcatcgta ctgcgggaac ggctgcagca
    16261 tgatggtgtc ggcagtgata ccgcaaagta ctttcacacg ctgccagatg gtttcggtga
    16321 tgaacggaat gatcggatgc gcgaggcgca gc
//
LOCUS       DACAPB010000097        16254 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.097, whole genome shotgun sequence.
ACCESSION   DACAPB010000097 DACAPB010000000
VERSION     DACAPB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.097"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..637)
                     /locus_tag="IT904_002795"
     CDS             complement(<1..637)
                     /locus_tag="IT904_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP3192552.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNTIFTVSEAMEHFAYLP"
     gene            complement(645..2993)
                     /locus_tag="IT904_002796"
     CDS             complement(645..2993)
                     /locus_tag="IT904_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP3192553.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINI
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSDWDKSGKLVKE"
     gene            complement(3171..4082)
                     /gene="gsiD"
                     /locus_tag="IT904_002797"
     CDS             complement(3171..4082)
                     /gene="gsiD"
                     /locus_tag="IT904_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAP3192554.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(4085..5005)
                     /gene="gsiC"
                     /locus_tag="IT904_002798"
     CDS             complement(4085..5005)
                     /gene="gsiC"
                     /locus_tag="IT904_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAP3192555.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(5023..6561)
                     /gene="gsiB"
                     /locus_tag="IT904_002799"
     CDS             complement(5023..6561)
                     /gene="gsiB"
                     /locus_tag="IT904_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAP3192556.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLDQ
                     ALKTNDPVEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(6581..8452)
                     /gene="gsiA"
                     /locus_tag="IT904_002800"
     CDS             complement(6581..8452)
                     /gene="gsiA"
                     /locus_tag="IT904_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAP3192557.1"
                     /translation="MPHSDELDAGDVLAVENLKIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAGVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(8439..9404)
                     /gene="iaaA"
                     /locus_tag="IT904_002801"
     CDS             complement(8439..9404)
                     /gene="iaaA"
                     /locus_tag="IT904_002801"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP3192558.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDASVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACEQVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            9608..10843
                     /gene="moeA"
                     /locus_tag="IT904_002802"
     CDS             9608..10843
                     /gene="moeA"
                     /locus_tag="IT904_002802"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAP3192559.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEVCEAVVMQEQTEQTDNGVRFTVEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGR"
     gene            10843..11592
                     /gene="moeB"
                     /locus_tag="IT904_002803"
     CDS             10843..11592
                     /gene="moeB"
                     /locus_tag="IT904_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAP3192560.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(11668..12330)
                     /gene="fsa"
                     /locus_tag="IT904_002804"
     CDS             complement(11668..12330)
                     /gene="fsa"
                     /locus_tag="IT904_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP3192561.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GITTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            12461..13360
                     /locus_tag="IT904_002805"
     CDS             12461..13360
                     /locus_tag="IT904_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAP3192562.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            13366..15798
                     /locus_tag="IT904_002806"
     CDS             13366..15798
                     /locus_tag="IT904_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAP3192563.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQMLEREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLIDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLAQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            15944..>16254
                     /gene="ybiV"
                     /locus_tag="IT904_002807"
     CDS             15944..>16254
                     /gene="ybiV"
                     /locus_tag="IT904_002807"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAP3192564.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     N"
BASE COUNT         4059 a         4403 c         3962 g         3830 t
ORIGIN      
        1 tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaaaatggtg tttttagcat
       61 taaattcatc accggtcacc ggtttaccgt tcttaaacag agagaacatc aggttctcgc
      121 tatccatcgc cagagatttt gacagtaaag aaaaacgctc cagaccaagc aaaaatatca
      181 gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg tcaaaagaga
      241 taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct gcacaataat
      301 ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa ggaccgacat
      361 cgtacaagta agagggtgaa accgagtcag cgtaagctgt gcgtaagcca accaacagct
      421 gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc cgatattcag
      481 caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcca tacacccgtt
      541 cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata aaaatcagca
      601 gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc ttttactaat
      661 tttccacttt tatcccagtc ggagaatggc atgggtttct ggtacagata cccctgagcc
      721 tgaacacatc ctaatttgat taattcgcgc gcaacgtctt cgctctctac gccttccgca
      781 atcaccggga tccggttata gcgtgccagg tcgaaaataa tgcgcacaat ttttcccgca
      841 gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctatctgg attaatttca
      901 taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat ccgtacccct
      961 tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc gatcacggtt
     1021 tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat gatggtggta
     1081 aaacgctcat gaaagtgcgg ttcgtttaac tgccatggcg aaatattaat atgcagtaag
     1141 aaatcatcct tcagcgacgc acgctgacta aaatgtctga attcacccat tgccgtttta
     1201 ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt aatgaacttc
     1261 agcggtgaaa tgatcccccg ctccgcagac acccaacggc acaacgcctc cgcgcccaga
     1321 atggcgcggg tgttaatatc aacgatcggt tgatagacca ggaatagttc gccaatttcc
     1381 attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt gagtttcgcg
     1441 tcatcacaga aaataaactc tgagaacata tggtcacgga tactggaaat cgccagtttc
     1501 agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat ggcagactta
     1561 tggatgcgca gattacggta gccaaagctg ctgtcaaaat cgtttaccat cgctatttta
     1621 cgggtaaatg tctgtacatt ttcccggcac agaagtaaat agagatcggc attgaatgtc
     1681 acgatatcgc agcattgcgc atatttttcg gcaattcgct gacacaattt cctgacgatt
     1741 ttattgcgtt catcaacacc aaacaccgct tccacggctt ccagaccaca cagtttgatg
     1801 atccccacag ccagatactg attattgttg gactcaataa tcgccatatt tttaataaaa
     1861 ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc aatttcggca
     1921 tctttcattt ccgcgatcga atttttaatg ctgataatct ctttcgcatc gccttcgcga
     1981 attttcagag cctcgatttg atccgttgag tcgggcaaaa atgccagcgt attaaccagc
     2041 tctgtcattt cagaaaattg ccgacggaac acacgagtgg tcattaaata aaccaccagc
     2101 aatacgataa taatgaacgc aaacggcaga taaaaaagtt tatctgaggc gttcaataaa
     2161 ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac tgctgatgtc
     2221 tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc gctattataa
     2281 ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc ttgtttcaaa
     2341 aatagaactt catcattagc gctgatttga tcaagatact cgttcagata ccgccagttt
     2401 aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt gatacgtagc
     2461 gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg attccagctg
     2521 ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa atcggcaaaa
     2581 ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg ataaacaacg
     2641 attttatcat tcgccctccg tgaaaagaga taatatttat tatcatcaag taccagtccc
     2701 accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg aatctcctct
     2761 gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc aatatcttta
     2821 aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg ttttaccgcg
     2881 cgttccgata ccagttgata gttaatgatg aaaaaaagac caataaatga cagtgctgcc
     2941 aataaaaata aaattatcag ccttatcttt atcttttcgt ataaactcag catgttaata
     3001 catttaacaa aataactgtc tgaacgctgt gccgctgcaa aaatagtccc ttgctgcggc
     3061 tttttaacca aattacccga ttctacaaaa tcagcagaaa gttacaacac ttgcttaggg
     3121 caataatttc ctgtgaggtg attacccttt caagcaatat tcaaacgtaa ctatccttta
     3181 attttcggat ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc cagtacggtc
     3241 agaaaaatag ccagggccgg aaaaatagcg acatgcggcg cgataaccat atccgctcgt
     3301 gcctcattga gcattgctcc ccactctggt gtcggcggtt gagcaccgag gccgagaaat
     3361 gacaggctgg cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa caccacgata
     3421 gacgagacgg tcccaggcaa gatatgacgc aataaaatgg tcatatcgct ggcaccaata
     3481 ctgcgtgctg actcaataaa ggtttgctgt ttcaacacca gcgtgttgcc gcgcaccagt
     3541 cgggcaaacg cggggatgga aaaaatggcg actgcgataa tcacgttagc aatgccgctt
     3601 cccaacaccg caacaacagc gatcgccagt aaaatacccg ggaaggcaaa cagcacatcg
     3661 caaatgcgca tgatcagccg atcccaccag ccttcataat atccagccag caagcccagc
     3721 aacgtcccga tcgccgcacc gataaacacg gcaaacacgc ccgccgccag cgagatttgc
     3781 gcaccaacca ggacacggct gaaaatgtca cgccccagtg aatcgacgcc aaaccagtgc
     3841 tgcaaagaag gtccgttatt cagattgtca taatcaaaat aattttcggc gtcataggga
     3901 gcgatccagc gtgcaaaaat agccaccaca atcaataaaa taacgaacaa tgcggcggtc
     3961 atcgccatat gctgacggcg aaatcgtcgc cagaattcat gccacggtgt acgtacctgg
     4021 tcaggtttga ccagtggcat ggcgtttaac accgcctgac gtcgccagtt aaatagtcgc
     4081 atccttactt gtacctgata gccgggttaa tggcggcgta aagcacatcc accactaagt
     4141 tgataagaat aaattccagc gagaaaagca gaatttccgc ctgaatcacc gggtaatcgc
     4201 gcatttctac ggagtcaacg agtaagcgtc caagtccggg ccagttgaaa actttctcca
     4261 ccacgataga accaccgagc aaaaagccaa actgtaagcc catcatggtc actaccggga
     4321 tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt ttcgctcacc cctttcgccc
     4381 tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa cgacgcgcgg gtaaagcgcg
     4441 ccatcacggc ggccactgcc gcgccgagcg tcagggaggg taaaatgtag tgctgccaac
     4501 tgtctgctcc cacggtaggc agccagccca gttcaacgga gaatacctga attaaaagca
     4561 tccccagagc aaatgccgga aacgagatcc ccgacaccgc aatggtcata ctcaatcgat
     4621 ccggccaacg attacgccag acggcggcga taattcccgc cgccatacca aatatcaccg
     4681 cccagaccat actggttatg gtcagccaca gcgttggcat aaagcggctg gcaatctcat
     4741 cggcaaccgg acgacgcgac accatcgaca ggccaaaatc cccctgcaca gcattgctga
     4801 tatagtgcca gaactggtga tacagcggct gatccaaccc cagctgctga cgcaccagtt
     4861 ctataacctg cgcatcagct tcgggcccgg caatcaatcg cgccggatcg ccgggcagca
     4921 tatgcacaaa taaaaacacc agcaccgaga cgataaacag cgttggaatc aaccccagta
     4981 agcgtttgat aacgtaatta agcattccac tccctgcgtt gcttattgca aatccgcgtc
     5041 ttcaaagctg aagccggtgt ctggcatgat ccaaaaaccg gtcaggtttt tactgtgtgc
     5101 cgacaccagt ttttctacca ccagcgggat ccacggcgat tcttgccaga tgatatcctg
     5161 cgccgcctta tataagcggg tcttttccac cggatcattc gttttcagtg cctgatccag
     5221 gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta aacagcgtcg gtggccagtt
     5281 ttgcgatgca aacagcggcg atagtgccca gtcagcttcg ccggttgaag ccgaccagcc
     5341 agtgtagaac atccgcacgc cgctctcttt ttgcccttta ccttcaactt ctgccgcccg
     5401 ctgtccggca tccatcgcag tcacctgggc tttaatcccg acctgcgcta actgctgctg
     5461 ggtaaattgc agcactttct gcgcggtgct gtggttatgt gacgaccaca gcgtggtact
     5521 gaaaccgttg ggatatcccg cctctttcag taattcgcgc gctttcactg gatcgtaagg
     5581 ccacggttta taactttgcg cgtaggcgat actcggcggt accacaccag tagctggcgt
     5641 tgcatagccc gcgaaggcta ctttcaccag cgccgggcgg ttaatggcgt aattcagcgc
     5701 ctcacggacc ttcgggttat cgaacggttt ttgcgtcacg ttcatactga tataacgctg
     5761 cataattgac ggactggcca tcaactcgat atttttgttt ttctccagca gtgcggcctg
     5821 ctcgtaagga atggggaaag caaactgcgc ttcaccggtt tgcagcattg ccgcgcgggt
     5881 gttgttatcc gccaccggac gccaggttat gctgtccagt ttgggcaatc ctggctgcca
     5941 gtaacccgcg aattttttca ccttcacaaa atcggtctga ttccaggtat ccagttcata
     6001 cggtccggtt cccaccggat gaaaaccaat ctccttgcca tatttttcca gcgctgccgg
     6061 tgaaatcatc gcggtcgccg gatgggcaag aatattaata aacgctgaaa acggctgttt
     6121 gagagtaatc tttaccgttg tcggatcgat cgcctccgtt ttggcaatat tcttatacag
     6181 gttatagcgt ttaagatgat tcgccggatc gctggcccgg tccagattcg cttttactgc
     6241 cgctgcgttg aaatcggtgc catcctggaa tttaatgcct tcccgcaatt tcacggtgta
     6301 tgtaaggccg tcatcggaaa cggtataact ctccgccagc acgtttttca gtttcatctc
     6361 tttatccaga ccaaacagcc cctggtaaaa cgatttcgct acggcctgag ataacgtgtc
     6421 atttgcgtca tacggatcga gcgtggtgaa gttcgatcct accgccacca ccacatcttt
     6481 ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca ctaacccact
     6541 acggtgtaca gctcttgcca ttttattctc cgcctgaatg ttatctacgc atgaatgcgt
     6601 attctgattg tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac gagacgcctg
     6661 ccacctcttc gccacgcaga tgaatattgc tgggaagatc gtccgacagc agtacacgct
     6721 gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc cagtaattta cgcgtataag
     6781 gatgctgcgg gttttcgaag accgcgcgcc gtgggccaat ttcaacaatt tgcccgagat
     6841 acatcaccgc cacacgatga ctaatccgct ctaccacggc catatcgtgg gagataaaca
     6901 gatacgcaat gccgaaatca cgctggagat cgagcaacaa gttgataatc tgcccgcgaa
     6961 tagaaacatc cagcgccgaa acggcttcgt cggcaatgat cacttttgga ttcaatgcca
     7021 acgcgcgagc aatgcagatg cgctggcgct gaccgccgga aaactcatgc gggtaacgcc
     7081 aggcatgttc aggtaacagg cccacgcgct ccagcaacca cgcaacgcgt gcagccgcgt
     7141 ctttacctgg caataaaccg tgtacgcgca gcggttcgat aatcgaatca ccgatggtct
     7201 gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa ctgaatatcc cggcgtaatg
     7261 cctgaagttt gccgggtgac aaggtatcga ttcgctgacc gttaaagata atttcgccgc
     7321 cctgcgattc gaccaggcgc agcaacgccc gcccggtagt ggatttaccg ctgccagact
     7381 cgcccaccag cgatagcgtt tcgccaggcc agagatcaaa actgactttc tcaacggcat
     7441 gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg gtgacaagat
     7501 tacgcacccg taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg gggggttcct
     7561 gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg gggataatct aaccctttca
     7621 tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt gtaaggatgt tgcggtgcat
     7681 gaaaaatctg ttcgacggta cccgtttcca ccgcctcgcc ctgatacatc accagtaccc
     7741 gatcggcaat ctctgccacc acgcccatat cgtgagtgat aaagataacg cccatcgaca
     7801 tctctttttg caatactttg attaattgca ggatctgcgc ctgaatagtg acatccagcg
     7861 cggtggttgg ctcatcggca atcagcaccg ccgggcggca tgacagcgcc atcgcaatca
     7921 tcactcgctg gcgcatcccg ccagagagtt gatgcggata acgtgaaaga atggtttgtg
     7981 cctccggaat gcgtacctga tccagcatcc gcttcgcctc gaccatcgct tcttcacgac
     8041 tggcgttctg atgcagacga attgattcgg caatctgttc acccacagta aataccgggt
     8101 tcagcgatgt catcggctcc tgaaatatca tcgccatatc cgcaccgcgc acatggcgca
     8161 tttgtgcagc gctctgctcg ctaagttcaa tcacttcacg actgcgccgc cgcaacagca
     8221 ttttatcgca ctgtactaaa ccgcccgcct gttccaacag acgcatcaat gccagcgcag
     8281 tcactgactt accggagccg gattcgccaa caatggccag cgtctcaccg cgttgaaggc
     8341 taaaagagag attgcggacc gcagctattt tctgctggtc ctgcataaag gcaattttca
     8401 gattttcaac cgccagcaca tcaccggcat caagttcatc actgtgtggc aacggtgtcc
     8461 cctttttcac ggtagatacc ggtggttggc gtatcgcctg cgtagcccca ggcgcgatac
     8521 attccttcgg tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc gattaagcca
     8581 ccgctaccgc caagcgcagg gagtttttcc attactacct gctcgcaggc ttccgcgaga
     8641 cttaatccgc cgtaatccat taacgcggcg atgtcatatg ccgccagcgc gcggatgaag
     8701 acttcgcccg tgccggtaca agaaaccgcc acactggcgt tattggcgta gcatccggca
     8761 cccactaagg gactatcgcc aactcgtccg ggtaatttat tggtcattcc gcctgtggac
     8821 gtggctgccg ccagattgcc gtctaaatcc aacgccacgg cccccacggt gcccattttc
     8881 tgtttttcat ccagtggcgc accgctatgg tcgaggactg ttgccccttc ctcgcgcgct
     8941 gccagtagtt gttcataacg caaaggcgtg gagaaaatct ccggtgagac gcgctccatg
     9001 ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca tcatcacatg cgggctttgc
     9061 tccatcacca gccgggcggc aagaaccgga ttacgcagat gactaacgcc cgccaccgca
     9121 ccggctttca gggtgttacc atccatcaca ctggcgtcca gttcatgggt ttcatcacgc
     9181 gtaaagacag cgccaattcc ggcgttaaac agtggacact cttccagcag acgcaccgct
     9241 tccgtcacca catccagcgc actttcgccc gcttccagca ttttctgccc ggtttcaaca
     9301 atggcagaca acgcctcaat gtagcgtaat tcctgttgca gactcatctg cgcgcggcta
     9361 attgcacctg cgccaccatg aattgcaatg actgctttgc ccatagtatt cgtccattaa
     9421 gggtggctcg ccacttttgc tataaatatc agaattttta tgaattactc attcaatttt
     9481 tgaatataga aagatggata tggcatgtaa aggcaggacc cagcgctacg cccgaggtgg
     9541 catacagtct gtggcgtttt tctgacataa taggcaaatt cgattttgcc tccgcaggag
     9601 tgttttcatg gaatttacca ccggattgat gtcgctcgac accgcgctta atgagatgct
     9661 ttctcgcgtc accccactga ccgcccagga aacgctgcca ctggtacagt gttttggtcg
     9721 tattctggcg agcgatgtcg tttcgccact ggatgtcccg gggtttgata actccgctat
     9781 ggacggctac gcggtgcgtt tagccgatat tgcctccggg caaccgctgc ccgttgccgg
     9841 gaaatcgttt gccggtcagc cgtatcatgg tgaatggcct gcgggcacct gcattcgtat
     9901 tatgaccggt gcgccggtgc cggaagtctg cgaagcggtg gtgatgcagg agcagactga
     9961 acaaacggac aatggcgtgc gttttactgt tgaagtgcgt agcgggcaaa atattcgccg
    10021 tcgcggtgaa gatatctctg cgggtgcggt tgttttcccg gcgggaactc gcctgactac
    10081 cgcagagctg ccagtaattg cttcactggg gattgccgaa gttccggtga ttcgtaaagt
    10141 gcgtgtagcg cttttttcta ccggtgatga actccagttg ccaggccagc cgctgggcga
    10201 cggccaaatc tacgatacca accgtctcgc cgtacacctg atgttagaac agctgggatg
    10261 cgaggtaatc aacttaggga ttatccgcga cgatccacat gccctgcgcg ccgcatttat
    10321 tgaagccgac agccaggcgg atgtggtgat cagttccggt ggtgtttcgg ttggtgaggc
    10381 ggattacacc aaaaccattc tcgaagaact gggcgaaatt gctttctgga aactggcgat
    10441 taaaccgggc aaaccgttcg cgtttggtaa actcagcaat agctggttct gcggcctgcc
    10501 gggcaacccg gtttcagcga cgctgacctt ctatcaactg gtacagcctt tgctggcaaa
    10561 actaagcggt aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca cagcgtcccg
    10621 cctgaaaaaa acgccaggac gtcttgattt ccagcgcggc gtgctgcaac gcaacgccga
    10681 tggcgaactg gaagtgacga ccaccggaca tcagggttca catatattca gctcctttag
    10741 cctcggcaac tgctttatcg tgctggaacg cgatcgcggc aatgtggacg tgggcgaatg
    10801 ggtggaagta gaaccgttta acgcgttgtt cggaggccgg taatggcgga actcagcgat
    10861 caggagatgc tgcgctacaa ccggcaaatc attctgcgcg gttttgattt tgacggtcag
    10921 gaggcactaa aagattctcg tgtgctggta gtcggcctcg gtggactcgg ctgcgcagcc
    10981 tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt cgacacggtt
    11041 tcgctctcga atctgcaacg ccagacgctg cacagtgatg ctacggtcgg gcaaccgaag
    11101 gtggaatccg cccgtgacgc cctggcgcgg atcaacccac atatcgcgat tacgccagtc
    11161 aatgcactgc tggatgacgc agaacttgca gcattgattg ctgaacacga tctggtgctc
    11221 gactgtacgg ataacgttgc ggtacgtaat caactgaacg caggctgttt tgccgcgaag
    11281 gtgccgctgg tttccggcgc ggcaattcgt atggaaggtc aaatcacggt cttcacttat
    11341 caggacggcg agccttgcta tcgctgcctc agccgtttgt ttggcgagaa cgcgttaacc
    11401 tgcgtagaag caggcgtgat ggcaccgttg atcggcgtaa ttggttcgtt gcaggcaatg
    11461 gaagcgatca aactgctggc aggttatggc aaaccggcca gcgggaaaat cgtcatgtac
    11521 gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg gtgtgaggtg
    11581 tgcgggcagt aattgccacc tccaggcctg ataagacgcg gcactccgcc acgtcatcag
    11641 caatttatga ggatgcagaa cgtccggtta aatcgacgtt ctgccaaacg cccccagcca
    11701 gtcctgctca aacttcgcca ccgcggcatc gacagccgga tagctaatca tctgttgtgc
    11761 cacatccagt ggcagagtaa ttgattcaca tcctgccagt aagcagtcca gcgcctgacg
    11821 cggggttttg aaactcgctg ccagcacttt cgcctgcggc gcatgcattt tcaataactg
    11881 gtgtaagtcg gtcacagtct gaatgccgct accgccctga gcatcaatac ggttgacgta
    11941 aggcgcaaca tattccgcac ctgccagcgc cgacagcagc ccttgtgccg cgccatatac
    12001 cgcggttccc agcgtcgtaa tcccttccgc ttttaacatc ttaatcgctg ccagcccctc
    12061 ggcggtcacc ggaactttca ccacgatatc cgcaataata gaacgcagct taagcgcgtc
    12121 attaaccatc ccttcggcag tggtagccat tacctgggca aacagacgcc cctgaccgcc
    12181 catcgcttca tgaagttccg gaagcacaac atccagcggt tttttacccg cggcgataat
    12241 gcttgggtta gtggtcacac ccgccagcgg aaaaatacgt gacagcgcct tcaccgcaac
    12301 aacgtctgaa gtatccagat acagttccat aaccatcctc aaaatgtact ttttgtctga
    12361 atgtagacta tcacgggaaa actctctgaa cagttgacgc acatcaatct aactttcatt
    12421 cgaaagtatt ttaatatttg aacgaaacag agaggcaact atgattttca atattcagcg
    12481 ctactcgacc catgatggcc ccggtatccg cacggtcgta tttcttaaag gctgttcgct
    12541 gggctgccgc tggtgtcaga acccggaaag ccgcgcccgc acgcaggatc tgctgtatga
    12601 cgcacgactg tgtctggaag gctgcgagct gtgcgctaag gccgcgccgg aagtgattga
    12661 gcgcgcgctg aatggtttgc ttattcatcg ggaaaagtta accccggagc atctgacggc
    12721 gttaaccgac tgctgtccga cacaggcgtt aaccgtgtgt ggtgaagtga aaagcgttga
    12781 ggagatcatg gcgaccgttt tgcgcgataa accgttttac gatcgcagcg gcggcggctt
    12841 aacgctttcg ggtggtgaac cctttatgca gccggagatg gcgacggcgc tactgcaagc
    12901 cagccacgag gcaggcattc atactgcagt agaaacctgt ctgcatgtgc cgtggaaata
    12961 tatcgcccct tctctgccct atatcgatct gtttcttgcc gatttaaaac acgttgccga
    13021 cgcgccgttt aaacagtgga ccgacggtaa cgccgccaga gtgctggata acctgaaaaa
    13081 actcgccgca gcgggcaaaa aaatcattat ccgcgtgccg ctgattcagg gctttaatgc
    13141 cgacgaaacc tctgtaaaag ccattaccga ttttgccgcc gacgagctgc acgttggcga
    13201 aattcatttc ctgccctacc acacgctggg catcaacaaa tatcacttac ttaatctgcc
    13261 ctatgacgcc ccggaaaaac cgcttgatgc gccagaactg ctcgattttg cccagcagta
    13321 tgcctgccag aaagggttaa ccgcgacctt acgaggataa caaccatgac cacactgaaa
    13381 ctggacacgc tcagcgaccg cattaaagcg cacaaaaatg cgctggtgca tattgtgaaa
    13441 ccgccagtct gtaccgagcg cgcgcagcac tataccgaga tgtatcaaca acatctcgat
    13501 aagccgatcc cggtacgtcg cgcgctggca ctggcgcatc acctggcgaa tcgcactatc
    13561 tggatcaagc acgatgagtt gatcattggc aaccaggcaa gcgaagttcg cgccgcgccg
    13621 atcttcccgg aatataccgt ctcctggatc gaaaaagaga ttgatgatct ggcagatcgt
    13681 ccgggtgctg gttttgcggt gagcgaagag aacaaacgtg ttctgcatga agtgtgccct
    13741 tggtggcgcg gtcagacggt acaggatcgt tgctacggca tgtttaccga tgagcaaaaa
    13801 ggtctgctgg cgaccggaat cattaaagcg gagggcaata tgacttccgg cgatgcgcac
    13861 ctggcggtca atttcccgct gctgctggaa aaagggcttg atggtctgcg cgagaaagtg
    13921 gcggaacgtc gctcgcgcat caacctgacg gtgctggaag atttgcacgg cgagcagttc
    13981 ctgaaagcga ttgatatcgt gctggtggca gtcagtgaac acattgaacg tttcgctgcc
    14041 ctggcgcgtg aaatggccgc gaccgaaacc cgcgaaagcc gtcgcgatga actgctggcg
    14101 atagcagaaa actgcgatct tatcgcccac cagccgccgc agacgttctg gcaggcgctg
    14161 caactgtgct acttcatcca gttgattttg cagattgaat ctaacggtca ctcggtgtcg
    14221 tttggtcgta tggaccagta tctctacccg tattatcgcc gtgatgttga actgaaccaa
    14281 atgctggaac gcgaacacgc catcgaattg ctgcatagct gctggttgaa attgctggaa
    14341 gtgaacaaga tccgctccgg ctcacactca aaagcctctg cgggtagccc gctgtatcag
    14401 aacgtcacca ttggcgggca aaatttgatt gatggtcagc cgatggatgc ggtgaatccg
    14461 ctctcctacg cgatcctcga atcctgcggt cgcctgcgtt cgacgcagcc taacctcagc
    14521 gtgcgctacc atgcgggaat gagtaacgat ttcctcgacg cctgcgtaca ggtgatccgg
    14581 tgcggcttcg ggatgccggc cttcaacaac gacgaaatcg tgatcccgga atttattaaa
    14641 ctcggtattg aaccgcagga cgcttacgac tacgcagcga ttggttgtat cgaaaccgcc
    14701 gtcggtggca aatggggcta tcgctgtacc ggcatgagct ttatcaactt cgcccgcgtg
    14761 atgctggctg cgctggaagg cggtcgtgat gccaccagcg gcaaagtgtt cctggcacaa
    14821 gaaaaagcgt tgtcggcagg taacttcaac aacttcgatg aagtaatgga cgcgtgggat
    14881 acgcaaatcc gttactacac ccgcaaatca atcgaaatcg aatatgtcgt cgacaccatg
    14941 ctggaagaga acgtgcacga tattctctgc tcggcgctgg tggatgactg tattgagcga
    15001 gcaaaaagta tcaagcaagg cggcgcgaaa tatgactggg tttctggcct gcaggtcggc
    15061 attgccaacc tcggcaacag cctggcggca gtgaagaaac tggtgtttga acagggcgcg
    15121 attggtcagc aacagctggc tgccgcactg gcggatgact tcgacggcct gactcatgag
    15181 caactgcgtc agcggctgat taacggtgcg ccgaagtacg gtaacgacga tgatactgtc
    15241 gatacgctgc tggctcgcgc ttatcagacc tatatcgacg aactgaaaca gtaccataat
    15301 ccgcgctacg gtcgtggtcc ggttggcggc aactattacg cgggtacgtc gtctatctcc
    15361 gctaacgtac cgtttggcgc gcagacgatg gcaacgccgg acggacgtaa agcgcatacc
    15421 ccgctggcag aaggcgcaag cccggcttcc ggtactgacc atctcggccc aacggcagtc
    15481 attggatcgg tgggtaaact gcctacagca gcgattctcg gcggcgtgtt gctcaaccag
    15541 aaactgaatc cggcgacgct ggagaacgaa tctgacaagc agaaactgat gatcctgctc
    15601 cgtaccttct tcgaggtgca taaaggctgg catattcagt acaacatcgt ttcccgcgaa
    15661 acgctgctgg aggcgaaaaa acatcccgat cagtatcgcg atctggtagt gcgtgtcgcg
    15721 ggctattccg ccttcttcac cgcgctctct ccagacgctc aggacgatat catcgcccgt
    15781 actgaacata tgctgtaatc tcctcggccc ggcggcgtcg tcgccgggct aaatcattca
    15841 catcatcaat ttcatcctta ctttcattcg aaatataatt tgtgctctac gtcacattgt
    15901 tattacactg ttacggtttc attttcgagc cgggagcaca cctatgagcg taaaagttat
    15961 cgtcacagat atggacggta cttttcttaa cgacgtcaaa acgtacaacc aaccacgttt
    16021 tatggcgcaa tatcaggaac tgaaaaagcg cggcattgag ttcgttgtcg ccagcggtaa
    16081 tcagtattac cagcttattt cattctttcc tgagctaaag gatgagatct cttttgtcgc
    16141 ggaaaacggc gcactggttt acgaacatgg caagcaactg ttccacggcg aactgacccg
    16201 acatgaatcg cggattgtta ttggcgagtt gctaaaagat aagcaactca attt
//
LOCUS       DACAPB010000098        16086 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.098, whole genome shotgun sequence.
ACCESSION   DACAPB010000098 DACAPB010000000
VERSION     DACAPB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.098"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            130..918
                     /gene="fabI"
                     /locus_tag="IT904_002808"
     CDS             130..918
                     /gene="fabI"
                     /locus_tag="IT904_002808"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP3192565.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1062..2189
                     /locus_tag="IT904_002809"
     CDS             1062..2189
                     /locus_tag="IT904_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAP3192566.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEEMLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFTDESIRWRSSYEV
                     EKLPEDYTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSLNEYKKINTLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2257..4191
                     /gene="rnb"
                     /locus_tag="IT904_002810"
     CDS             2257..4191
                     /gene="rnb"
                     /locus_tag="IT904_002810"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAP3192567.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4268..4372)
                     /locus_tag="IT904_002811"
     CDS             complement(4268..4372)
                     /locus_tag="IT904_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192568.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            4426..6411
                     /gene="pdeR"
                     /locus_tag="IT904_002812"
     CDS             4426..6411
                     /gene="pdeR"
                     /locus_tag="IT904_002812"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP3192569.1"
                     /translation="MKTVRESTTLYNFLGSHNSYWRLTESSDVLRFSTTETTEPERML
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            6558..6731
                     /locus_tag="IT904_002813"
     CDS             6558..6731
                     /locus_tag="IT904_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192570.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            6821..7570
                     /locus_tag="IT904_002814"
     CDS             6821..7570
                     /locus_tag="IT904_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP3192571.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            7839..8057
                     /gene="osmB"
                     /locus_tag="IT904_002815"
     CDS             7839..8057
                     /gene="osmB"
                     /locus_tag="IT904_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAP3192572.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8183..8509)
                     /gene="yciH"
                     /locus_tag="IT904_002816"
     CDS             complement(8183..8509)
                     /gene="yciH"
                     /locus_tag="IT904_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAP3192573.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8509..9246)
                     /gene="pyrF"
                     /locus_tag="IT904_002817"
     CDS             complement(8509..9246)
                     /gene="pyrF"
                     /locus_tag="IT904_002817"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP3192574.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(9439..10608)
                     /gene="lapB"
                     /locus_tag="IT904_002818"
     CDS             complement(9439..10608)
                     /gene="lapB"
                     /locus_tag="IT904_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAP3192575.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(10615..10923)
                     /gene="lapA"
                     /locus_tag="IT904_002819"
     CDS             complement(10615..10923)
                     /gene="lapA"
                     /locus_tag="IT904_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAP3192576.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(11072..11836)
                     /gene="pgpB"
                     /locus_tag="IT904_002820"
     CDS             complement(11072..11836)
                     /gene="pgpB"
                     /locus_tag="IT904_002820"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAP3192577.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            12006..12596
                     /gene="ribA"
                     /locus_tag="IT904_002821"
     CDS             12006..12596
                     /gene="ribA"
                     /locus_tag="IT904_002821"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAP3192578.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNENYLDTKAEKMGHLLNK"
     gene            complement(12660..15335)
                     /gene="acnA"
                     /locus_tag="IT904_002822"
     CDS             complement(12660..15335)
                     /gene="acnA"
                     /locus_tag="IT904_002822"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAP3192579.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(15499..15594)
                     /locus_tag="IT904_002823"
     CDS             complement(15499..15594)
                     /locus_tag="IT904_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192580.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(15708..15875)
                     /locus_tag="IT904_002824"
     CDS             complement(15708..15875)
                     /locus_tag="IT904_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192581.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(15878..16006)
                     /locus_tag="IT904_002825"
     CDS             complement(15878..16006)
                     /locus_tag="IT904_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAP3192582.1"
                     /translation="MPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKVWG"
BASE COUNT         4045 a         4153 c         3840 g         4048 t
ORIGIN      
        1 gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt atctgttcgt
       61 actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg
      121 attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa
      181 ctatccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc
      241 acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct
      301 gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat gttcgctgaa
      361 ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc
      421 gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa aattgcccac
      481 gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg
      541 ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac
      601 gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg
      661 ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac tctggcggct
      721 tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc
      781 cgtaccgtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc cgatctctct
      841 gccggtatct ccggtgaagt agtccacgtt gacggcggtt tcagcatcgc tgcaatgaac
      901 gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg ccgcccgtta
      961 tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct
     1021 tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca
     1081 ccggcaaaag ccactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc
     1141 ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg
     1201 aagaaatgct ggcccccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggaat
     1261 tgttccctga ccagcttacc gtgacccgtt cacagacttt caccgcttat gaacgcttgt
     1321 ctacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac tactattcgg
     1381 cgcgacttac gccgctcccc gggcctgatt cctccaggga aagtaatcat cggttggcac
     1441 aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc
     1501 gccagcatct gaatgacgtc ggtcttactg cctgggactg tgtaatcatt aaccaaatca
     1561 ttggatttat tggctttcag gcgcggacca ttgcgacatt tcaggcttat cttggacatc
     1621 cggtacgctg gttacccggt ctggagatac aaaactatgc cgacgcgtca ctgtttactg
     1681 atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa gattacacaa
     1741 aaagttcaac tgcagaactt tgccaactgg ctgaaacact ctctctccac cctatttcac
     1801 tttcccttct tgaaaagttg ttaaacagca cacgggttaa tacacagccg gataatcagc
     1861 ttgcggcgtt gttatgcgca cggataaatg gcagccctgc ttgttttgcc gcctgtatgg
     1921 attcattaaa tgaatataaa aaaatcaaca cccttctgcg caagggcgaa aatgaaatta
     1981 accaatgggc tgaccgtcat tctgttgagc acgctaccgt tcaggcgata caatggctga
     2041 cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa cacgaaaaat
     2101 catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct
     2161 taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac
     2221 tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta
     2281 gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc
     2341 acagaaaaag gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg
     2401 ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca cagtgaaaaa
     2461 gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt
     2521 aaggttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact cttaaaagac
     2581 gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg
     2641 gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc agaactgaca
     2701 caatacatca cttttggtga cgatcacttt gtaccgtggt gggttaccct tgcacgccat
     2761 aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt
     2821 cgtgaagatc tgaccgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg
     2881 gatgacgccc ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat tgtggcgatt
     2941 gccgatccaa ccgcgtggat tgctgaaggc agtaagctgg acaaagccgc gaaaattcgc
     3001 gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac
     3061 gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc
     3121 tccgctgatg gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa
     3181 gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga ctggaagcct
     3241 gaaagtgaag caattgccga acaagtccgt ttgttagcgc aaatttgcca acgccgcggc
     3301 gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc
     3361 ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc
     3421 gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc
     3481 ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca
     3541 gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac gctggacggt
     3601 ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt
     3661 cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt
     3721 ctggaagcat acgccacctg gacatcgccg atccgtaaat atggcgacat gatcaaccac
     3781 cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccacagga tgagatcact
     3841 gtccaaatgg ccgagcgtcg ccgtctcaat cggatggcag aacgtgatgt tggtgactgg
     3901 ttatacgcac gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc ggcggaaatt
     3961 gtcgatatca gccgtggcgg catgcgtgtt cgtttggttg ataacggcgc tatcgccttt
     4021 attcctgcac ctttcttaca cgctgtgcgc gatgaaatgg tttgcagcca ggaaaacggc
     4081 accgtacaaa ttaaaggtga aacggtttat aaagtaactg acgttattga cgtcaccatt
     4141 gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc
     4201 acggcccatt ccttatgaat gggccgttta tttccccgct ctaccttcat catatcccgg
     4261 cagtttttta actgactttc gtttgaaaac tggcgttttt ccaaaactgg attacaaata
     4321 aatatataaa aaaaacaatg cgtttagccg ccgttaattt catgtaagtc atgttgttcc
     4381 ttttatttaa ccgcggactc cgctgttaac cggaggatat gcatcatgaa aaccgttagg
     4441 gagtccacaa cgttgtacaa ctttctcgga tcgcacaatt catactggcg gttgacggaa
     4501 agcagcgatg ttttgcgctt ttctaccacc gaaaccacag aacctgaacg tatgttgcag
     4561 ttatctgccg aacaggctgc tcgaatcagg gaaatgacgg tcatcacctc cagcctgatg
     4621 atgagtctga ccgtcgatga aagcgatctt tctgtgcatc tggtaggacg aaaaatcaat
     4681 aaacgggaat gggctggcaa cgcgtctgca tggcatgaca caccggcggt tgctcgtgat
     4741 ttatcacacg ggctttcctt tgctgagcag gtagtttctg aagcacattc cgcaatagtc
     4801 attctcgaca gccgggggaa tattcaacgc ttcaatcgct tatgtgaaga ttacacaggg
     4861 ttgaaagaac acgacgtcat tgggcaaagc gtgtttaaac tgtttatgag ccgtcgtgaa
     4921 gctgcggcat ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc
     4981 gaactgtgga taccaacacg taaaggccag cggctgtttc tgtttcgcaa taaatttgtc
     5041 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
     5101 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
     5161 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcatgc agataacaat
     5221 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
     5281 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
     5341 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
     5401 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
     5461 cgtattggtt taattgaagt ttataccagc tgttcagtag gtattgcact ctctcccgaa
     5521 catggttcgg acagcgcggc tattattcgt cacgccgaca cagcaatgta cacagcgaag
     5581 gaaggcggac gaggacaatt ttgcgttttt accccagaaa tgaatcaacg ggtatttgaa
     5641 tatctctggc tggataccaa cttgcgtaaa gcactagaaa acgatcagtt ggttattcac
     5701 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
     5761 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
     5821 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
     5881 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
     5941 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
     6001 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
     6061 gttattcaac aatttagcca actaggtgcg caagtacatc tggatgattt tggtaccggc
     6121 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
     6181 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
     6241 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
     6301 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
     6361 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
     6421 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
     6481 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
     6541 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
     6601 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
     6661 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
     6721 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
     6781 tttttttatt aagtattata acgttataag aattacagcg atgaattccc gacaacaaac
     6841 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
     6901 tggagtttct gaagttacca ttcgccagga tctcaacaca ctcgaaaaac tgagttacct
     6961 ccgccgtgca catggctttg ctgtttcgct tgatagtgat gacgtcgaaa cccgtatgat
     7021 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcgctgg ttcaaccggg
     7081 cgaaaccatc tttatcgaaa atggcagcag caatgcccta ctcgcccgga ctctgggcga
     7141 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
     7201 cgcgccttgt gaagttattt tgctcggtgg cgtgtatcag aaaaaaagcg aaagtatggt
     7261 tggccctttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
     7321 tgatggctgg caacctgaaa ctggatttac cggtcgcgat atgatgcgta ccgatgtggt
     7381 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
     7441 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
     7501 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
     7561 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
     7621 aagatgattc ctggttgata attaagacta tttacctgtt attaacactc tcaagatata
     7681 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
     7741 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
     7801 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
     7861 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
     7921 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
     7981 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
     8041 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
     8101 tgattctgtt cagtacgaaa taactgaata atacacacag taaaatattc gaaagccacg
     8161 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
     8221 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
     8281 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
     8341 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
     8401 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
     8461 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
     8521 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
     8581 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
     8641 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
     8701 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
     8761 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
     8821 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
     8881 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
     8941 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
     9001 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
     9061 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
     9121 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
     9181 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
     9241 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
     9301 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
     9361 ttctgcgccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
     9421 gtaggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
     9481 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
     9541 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
     9601 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
     9661 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
     9721 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
     9781 caccgcacgc tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
     9841 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcag
     9901 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcattatgga
     9961 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    10021 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    10081 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    10141 ctcactggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    10201 ttcatcggtc agctgattga acatgtcttc cgcgcggtca tataacccgg cggccatgta
    10261 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagcg aggcgctttc
    10321 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    10381 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    10441 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    10501 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    10561 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    10621 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    10681 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    10741 gccacaaatc aaccaaccga tagcaaaccc cgcagcaaac aataccgcca gcaatgtgga
    10801 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    10861 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    10921 cacattactt cccgttatgt ggttagagcg aataaattat ggtcaactta ccgcaatagc
    10981 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacacgcat tgttgatgat
    11041 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    11101 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    11161 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    11221 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    11281 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    11341 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    11401 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    11461 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    11521 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaagga
    11581 ttgcggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    11641 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    11701 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    11761 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    11821 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    11881 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    11941 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    12001 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    12061 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    12121 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    12181 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    12241 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    12301 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    12361 accagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    12421 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    12481 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    12541 acgaaaatta tctcgatacc aaagccgaga aaatgggcca tttgctgaac aaataaccct
    12601 cttgcattgt gtaattcatt tgcttgccgg aagcaaaata accggcaagc aaatagttgt
    12661 tacttcaaca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    12721 tccgtcgcgg tgtcgatacg acaacggcag ggtacgactt cctggctacc atccgcgcgc
    12781 gtaagcgtca ccggaaccgt cgcgccgggt tgtaggtttt gcagatcgcc aatatcaatc
    12841 ttctcttccc cggttagccc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    12901 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    12961 acacgaatac caagcagacg cggacctttt gccgcccagt cacgactgga gcctgatcca
    13021 tactctttcc cggcaatcac cgccagcggc gtttgctcct gcttatagcg catcgcagca
    13081 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    13141 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    13201 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    13261 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    13321 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    13381 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    13441 ccgtaggtat cggatcgtgt gacgttaatt cccttccact ctgctgtgcc ttcaaaaact
    13501 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    13561 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttgcgatc atggccgata
    13621 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    13681 ggcgaggcca gccagttagt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    13741 ccggacagca ccgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    13801 ggcagcggcc cagagttacc aatacaggtg gtacaaccgt atcccacaag gttaaacccc
    13861 agttcgtcga gatacggtgt cagtttcgct tttgccagat aatcagaaac gactttcgaa
    13921 cccggtgcca gcgacgcttt gacccatggt tgccgcttga ggcccagagt tacggctttt
    13981 ttcgccagca agcctgcggc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    14041 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    14101 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    14161 gcttttggta catcgggcag tgcaacgcga tcctgtgggc gtttaggccc tgccaggctc
    14221 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaattggttc atcgcccggg
    14281 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcgacctg atcttcgctg
    14341 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    14401 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccaacgg tagtgaatcc
    14461 agaccatcac cataaaattc gacgaatttc cccaccacgc catgtttgcg cagcatttgg
    14521 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaattttcc ggtaagtttg
    14581 aagcccacta catccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    14641 tcgatcccac caacgcccca ccccagcacg ccaaggccgt tgatcatggt ggtgtgcgag
    14701 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    14761 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    14821 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    14881 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    14941 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    15001 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgcca
    15061 gtaaagtcct gcatcagcac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    15121 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    15181 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    15241 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    15301 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    15361 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    15421 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    15481 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    15541 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    15601 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    15661 taaaagccct ttaattcatt aaaaatgagt aataagaatg atgataatta ttgtttcggc
    15721 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    15781 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    15841 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    15901 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    15961 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    16021 aatcgccatc acttatcaga aagacgttga acgattggtg taattaatca tacgatgaga
    16081 caaatc
//
LOCUS       DACAPB010000099        16065 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.099, whole genome shotgun sequence.
ACCESSION   DACAPB010000099 DACAPB010000000
VERSION     DACAPB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.099"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1212
                     /gene="dnaE"
                     /locus_tag="IT904_002826"
     CDS             <1..1212
                     /gene="dnaE"
                     /locus_tag="IT904_002826"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time; main
                     replicative polymerase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP3192583.1"
                     /translation="KSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVVGLV
                     DECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKGGYF
                     RELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAKAEA
                     IGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKEIER
                     YVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVMLFTD
                     ALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISLTDR
                     QIDDQLLNRLRQSLEPHRSGAIPVHLYYQRADARARLRFGATWRVSPSDRLLNDLRGL
                     IGSEQVELEFD"
     gene            1225..2184
                     /gene="accA"
                     /locus_tag="IT904_002827"
     CDS             1225..2184
                     /gene="accA"
                     /locus_tag="IT904_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP3192584.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2283..4424
                     /gene="ldcC"
                     /locus_tag="IT904_002828"
     CDS             2283..4424
                     /gene="ldcC"
                     /locus_tag="IT904_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAP3192585.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALLAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFSAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4481..4870
                     /locus_tag="IT904_002829"
     CDS             4481..4870
                     /locus_tag="IT904_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP3192586.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4935..6233
                     /gene="tilS"
                     /locus_tag="IT904_002830"
     CDS             4935..6233
                     /gene="tilS"
                     /locus_tag="IT904_002830"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP3192587.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQVRYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGVDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYDETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6282..6542)
                     /gene="rof"
                     /locus_tag="IT904_002831"
     CDS             complement(6282..6542)
                     /gene="rof"
                     /locus_tag="IT904_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAP3192588.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6529..6729)
                     /locus_tag="IT904_002832"
     CDS             complement(6529..6729)
                     /locus_tag="IT904_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAP3192589.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6895..7440
                     /locus_tag="IT904_002833"
     CDS             6895..7440
                     /locus_tag="IT904_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAP3192590.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7437..7859
                     /gene="arfB"
                     /locus_tag="IT904_002834"
     CDS             7437..7859
                     /gene="arfB"
                     /locus_tag="IT904_002834"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP3192591.1"
                     /translation="MIVISRHVAIPDNEYEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLVSKAQKSSVKALRGKVRSGRE"
     gene            7873..8583
                     /gene="nlpE"
                     /locus_tag="IT904_002835"
                     /gene_synonym="cutF"
     CDS             7873..8583
                     /gene="nlpE"
                     /locus_tag="IT904_002835"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAP3192592.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8783..9606)
                     /locus_tag="IT904_002836"
                     /pseudo
     CDS             complement(8783..9606)
                     /locus_tag="IT904_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(9660..11378)
                     /gene="proS"
                     /locus_tag="IT904_002837"
     CDS             complement(9660..11378)
                     /gene="proS"
                     /locus_tag="IT904_002837"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP3192593.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11489..12196)
                     /gene="tsaA"
                     /locus_tag="IT904_002838"
     CDS             complement(11489..12196)
                     /gene="tsaA"
                     /locus_tag="IT904_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAP3192594.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLMAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12193..12597)
                     /gene="rcsF"
                     /locus_tag="IT904_002839"
     CDS             complement(12193..12597)
                     /gene="rcsF"
                     /locus_tag="IT904_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAP3192595.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12715..13530)
                     /gene="metQ"
                     /locus_tag="IT904_002840"
     CDS             complement(12715..13530)
                     /gene="metQ"
                     /locus_tag="IT904_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAP3192596.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13570..14223)
                     /gene="metI"
                     /locus_tag="IT904_002841"
     CDS             complement(13570..14223)
                     /gene="metI"
                     /locus_tag="IT904_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAP3192597.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14216..15247)
                     /gene="metN"
                     /locus_tag="IT904_002842"
     CDS             complement(14216..15247)
                     /gene="metN"
                     /locus_tag="IT904_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAP3192598.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15435..16007
                     /gene="gmhB"
                     /locus_tag="IT904_002843"
     CDS             15435..16007
                     /gene="gmhB"
                     /locus_tag="IT904_002843"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP3192599.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         3899 a         4047 c         4312 g         3807 t
ORIGIN      
        1 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac
       61 tatccggcag agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
      121 gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
      181 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcg
      241 atcaaagggg tcggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
      301 tacttccgcg aactgtttga tctctgcgcc cgaaccgaca ccaaaaagtt aaaccggcgg
      361 gtgctggaaa aactgattat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggca
      421 ctgatgaact cgctgggcga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
      481 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
      541 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
      601 ggtctgtacc tgacgggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
      661 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg
      721 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
      781 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
      841 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
      901 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
      961 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
     1021 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg gggcaattcc agtacatctc
     1081 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
     1141 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
     1201 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
     1261 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
     1321 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacacgt
     1381 aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccacagcgt
     1441 ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
     1501 cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg
     1561 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
     1621 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
     1681 tttaagatgc caatcatcac ctttatcgac accccggggg cttatccggg cgtgggcgca
     1741 gaagagcgcg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttagc
     1801 gtaccgacta tttgtaccgt tatcggtgaa ggtggttctg gcggcgcgct ggcgattggc
     1861 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
     1921 tgtgcgtcca ttctgtggaa gagcgctgat aaagcgccgc tggcggctga agcgatgggt
     1981 atcatcgctc cgcgtctgaa agaactaaaa ctgatcgact ccatcatccc agaaccgctg
     2041 ggtggtgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgttggcg
     2101 gatctggccg atctcgacgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
     2161 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
     2221 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
     2281 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
     2341 aagaactgga gtcggcgctg ctggcgcaag gctttcagat tatctggcca caaaacagcg
     2401 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
     2461 atgagtacag tctcgattta tgtagcgata tcaatcagct taatgaatat ctcccgcttt
     2521 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
     2581 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
     2641 acaccgacga atatcttgat aacattacac cgccgttcac gaaagccttg tttacctacg
     2701 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggtacc gcctatcaaa
     2761 aaagcccggt tggctgtctg ttttatgatt ttttcggcgg gaataccctc aaggctgatg
     2821 tctctatttc ggttaccgag cttggttcgt tgctcgacca caccgggcca cacctggaag
     2881 cggaagagta catcgcgcgg acttttagcg cggaacagag ttatatcgtt accaacggaa
     2941 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
     3001 tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat gtagtgccag
     3061 tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg cgccgtgaat
     3121 ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa tggccggttc
     3181 atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac tggatcaaac
     3241 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac acccattttc
     3301 atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga aaagtgatct
     3361 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg ctgatccaca
     3421 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
     3481 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
     3541 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
     3601 agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
     3661 atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt aacgatgcgg
     3721 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
     3781 aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa ttcctcgacg
     3841 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
     3901 gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aaacgctctt
     3961 acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa gatcccgatt
     4021 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
     4081 acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
     4141 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
     4201 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
     4261 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
     4321 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
     4381 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
     4441 ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
     4501 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
     4561 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
     4621 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
     4681 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
     4741 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
     4801 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
     4861 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
     4921 aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc
     4981 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
     5041 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
     5101 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
     5161 tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg
     5221 tgcgttatca ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc accgcgcaac
     5281 atctcgacga tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc ggtcctgccg
     5341 ggctttcggc tatgggggaa gtttcggagt ttgccggaac acggcttatc cgcccgttgc
     5401 tcgcccgcac gcggggggaa ctggagcagt gggcattagc gcatggttta cgctggattg
     5461 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgtgtagtgc
     5521 cgttattgca gcagcgttgg ccgcattttg ccgaagcaac ggctcgcagc gccgcacttt
     5581 gtgctgaaca agagagcctg ctggatgaat tgctggcaga tgatttagcg cactgccaaa
     5641 cgccgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg
     5701 caattatccg ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg
     5761 tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt ttacgctttg
     5821 gcgcgtttga aatacgccgc tatcaatcgc aactgtggtg gattaaatcc gttgccgggc
     5881 aaagcgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta ccggcggggc
     5941 tgggaagtgt acagcttact gcgggagtcg atattcgccc tccgcgtgca gacgaagcgg
     6001 tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cggtcgcaac ggcggacgta
     6061 agctaaagaa aatctggcaa gagctgggcg tgccgccgtg gctacgtgac accacgccac
     6121 tgctgtttta tgacgaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
     6181 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
     6241 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggactc gctcaccacc
     6301 accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag ctcacgggtt
     6361 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcgctggct
     6421 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
     6481 gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc attcattgac
     6541 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
     6601 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
     6661 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
     6721 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
     6781 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
     6841 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
     6901 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg taaccagttt
     6961 ctcgatgcct ctctgacgct ggcgcgccat ccttcagaaa cccaggagcg tatgatgctg
     7021 cgcttgctgg cgtggctgaa atatgccgat gaacgtctgc aatttacccg tggtttgtgt
     7081 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
     7141 gagctggggc tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg
     7201 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
     7261 tgtgcgcagt ttgccaatct ttccgtctgg tatctggacg acgagcagct ggcgaaagtg
     7321 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg cgttatctgg
     7381 ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca accttcatga
     7441 ttgtgatttc ccgacatgtc gctatccctg ataatgagta tgaaatcacc gccattcgtg
     7501 cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat ctgcgttttg
     7561 acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
     7621 atttgatcag cagtgatggc gtgattgtca tcaaggcaca ggaataccgc agccaggaac
     7681 tgaaccgcga agcagctctg gcccggctgg tggctgtgat taaagattta acaacagaac
     7741 aaaaagcccg acgacccacg cgacccaccc gcgcatcgaa agagcgtagg ttggtatcga
     7801 aagcacaaaa atcaagcgtg aaagcgttgc gcggcaaagt gcgcagcggt cgggaataaa
     7861 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
     7921 acactgatgg gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc
     7981 gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga ttgcgaaggg
     8041 atcgaaacct ctttgtttct tgaaaaagac ggaacatggg tgatgaacga acgttatctg
     8101 ggcgcgcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
     8161 aagttggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
     8221 ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta tacgctggaa
     8281 ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
     8341 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
     8401 gcagagctgg agcgtggcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
     8461 tcagtagaag gtcactttac gcttgagggt aatccggata ccggtgcgcc gactaaagta
     8521 ttggcacccg atacggcagg gaaattttac cccaaccagg attgcagtag tttggggcag
     8581 taacccgtct tgagacagaa acaaacgcaa aacaggccag aaggatatat ttcaacattt
     8641 tgaatttgca cgttttttgt aggccggata aggcgtttac gccgcatccg gcaatggtgc
     8701 tcaacgcctg atgcgacgct ggcgcgtctt atcatgccta caacccccct cataccttaa
     8761 ggcggataag gcatttactt tatcaccggg tcaaaccaac aaagcgaccc gccttgatat
     8821 aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta aacgcagaga
     8881 catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat tgcgccagtg
     8941 taagtggatg cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa aaccacgact
     9001 ttttgtctcc ttcgcccaca ctgcttaact gcactttcgc caggccgctg acgcactttg
     9061 gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca taaagggaat
     9121 aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg caaacgcggt
     9181 gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa gcttatcact
     9241 atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac
     9301 gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga agacgcggat
     9361 tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg
     9421 tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga atttgacagc
     9481 ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc tgatatcaac
     9541 ggtacaagcc gaaagtaaaa gaaagctggg gagaaacagg cggcagtacg cctttggttt
     9601 atccatttta tacaatccat gtaaaaaaag ggccctgaaa ttcaggaccc tttctggcat
     9661 cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat taactgtttc
     9721 tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg gtcgcccagc
     9781 acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc cggacgctct
     9841 ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta cagtttctca
     9901 gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc cacctggaac
     9961 ggcgcgatag cgtcaggcca tacgatgcct cgttcgtcgt agttctgctc aatcgccgca
    10021 gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg gttacggcca
    10081 tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag ctggaagatg
    10141 tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg gcttggatcg
    10201 ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg atcccagttg
    10261 atgccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact catcgccgca
    10321 acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga acccggaccg
    10381 gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct tgcaacctgc
    10441 ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag cagcgcaacc
    10501 agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt tttctcaatc
    10561 ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt atcaaccagc
    10621 gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc tgccagttca
    10681 atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc gctctgcgcc
    10741 agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc ctgtacggcg
    10801 cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat tgcatcgtag
    10861 gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat caggaattcg
    10921 cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt ctggatctga
    10981 tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag gtcagtgata
    11041 acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac aaaacgcagc
    11101 agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc tggctgaacc
    11161 accggcatcg acacctcgat tgcaccggcg ttgttcatct cttcacgcac gatgttttcg
    11221 acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga ggccagcttg
    11281 cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc ggcaggtgtc
    11341 tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt ccagttggaa
    11401 ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta ccagtgcaaa
    11461 agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag acttcaaaac
    11521 ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca taggttttgc
    11581 ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact tcgcggataa
    11641 acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg acctctgcgg
    11701 taaaactcac cgccatttct gccgctggcg cactttgcgc atagctggca ctggcatcgg
    11761 gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta ccatcgacca
    11821 gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc tctttcagct
    11881 ctaccagcga cataccaatt gggttagggc ggaaagtaga gcgcgtagcg aaaacaccca
    11941 ttctggcatt accgccaaga cgaggcggac gcacagtcgg acgccagccg ccttccatcg
    12001 tttgatgaaa gacgaaaagt atccataaat ggctgaacgc ttccaggccg cgaacggcgt
    12061 cggcctggtt gtagggagcc atgagatgca gttcgccgtt ggcgcttttt accagacctg
    12121 gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct atttgctcga
    12181 actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata cagcctgacg
    12241 atagcagcct ggcgtaccgc tggtaacttc gcagctatgc agtaataccg cattggcttt
    12301 cattttcgag gcgttgattt gcatccgctt acgtgcggtt ggaatgctcg gcggagagtc
    12361 ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga acggtttgcc
    12421 gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg ctttcggttt
    12481 tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag gggatctgct
    12541 taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag cacgcatagt
    12601 atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa aaatgacaag
    12661 acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta aaaattacca
    12721 gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt catcagactg
    12781 ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac gcgttacgat
    12841 caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg ctggagtcag
    12901 gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg cgtcgtccag
    12961 agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg ggttctcaac
    13021 aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca agcccacttt
    13081 ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg caacctgcga
    13141 accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa tcggataaac
    13201 aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct gatcaaggta
    13261 cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt cgtttggcag
    13321 aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt tcgcaacttt
    13381 ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt taatgtggtt
    13441 tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca gggctcccac
    13501 tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt atttatgttg
    13561 tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga actgaattaa
    13621 ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt tgtagccgat
    13681 gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac cacccatcgc
    13741 ggaataaccg actagggtaa tcagggtgat agttgccgca ttcaccagac ccggcaacgc
    13801 ttccggtaac agcactttac ggacgatctg cattggcgtg gcacccattg cgcgggaagc
    13861 ttcaattaac ccggttggga tctccagaag agcgttctcg accatacggg caataaacgg
    13921 cgctgcgcca acggttaacg gaacaatcgc tgcctgcaaa ccaatcgatg taccgacaat
    13981 aactcgggta aacggaatca tccataccag caagataatg aacgggatgg aacggaaaat
    14041 gttcacaatt gcagaaacgg tacgatacag cttcgcgtta gcaataattt gccccggacg
    14101 cgtgacataa agtaggacgc caaccggcag accaatcaca aagccaaaaa atccggatac
    14161 gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca tcggctcaga
    14221 cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg gcggcttgcg
    14281 tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttaacg ccaccggcgt
    14341 aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg gtttcagaaa
    14401 gcagtggggc atcgaccgat tgaccggtaa actccagacg caacatcggc acgcagtcag
    14461 tgaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga tgcagggtag
    14521 actgaataaa cttctgcgcc agcggcgttt tcggatgtga gaacacttca cttaccgtgt
    14581 cctgctcgat cagttcgccg ttgctgatga ccgccacgca atcacaaata cgcttcacaa
    14641 cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggttg atgtctttca
    14701 gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct tcatcacaca
    14761 gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt ttctgcccac
    14821 cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat gacagcaatt
    14881 ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc ggcagagcca
    14941 cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc ataccaatct
    15001 ggcggcgagc tttggtcaac tcagattctg acagcgtggt cagctcctgg ccatcgacca
    15061 gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc gtactcttac
    15121 ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc aggctgacgt
    15181 tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata ttcgaaagtt
    15241 ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatttcgaa tgcctgaaac
    15301 gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc aatcaggttt
    15361 atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtcaca tgccttatta
    15421 aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt gatggcacca
    15481 ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc gacggtgtta
    15541 ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta accaaccagt
    15601 ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc gagtggatgg
    15661 actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc ccgcatcatc
    15721 cgcagggtag tgttgaagag tttcgccagg tctgcgactg ccgtaaacca catccgggga
    15781 tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat atggtgggcg
    15841 ataaattaga agatatgcag gcagcggctg cggcgaacgt gggaacaaaa gtgctggtgc
    15901 gtacgggtaa acctattacg cctgaagcag aaaacgcggc ggattgggtg ttaaatagcc
    15961 tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcataatgg ttaaaagatg
    16021 agcgattgaa ataaaaatgc atttttccac ttgtcttcct gagac
//
LOCUS       DACAPB010000100        16047 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.100, whole genome shotgun sequence.
ACCESSION   DACAPB010000100 DACAPB010000000
VERSION     DACAPB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.100"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..151
                     /locus_tag="IT904_002844"
     CDS             <1..151
                     /locus_tag="IT904_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HAP3192600.1"
                     /translation="LMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDF
                     NDFKE"
     gene            154..552
                     /gene="yafO"
                     /locus_tag="IT904_002845"
     CDS             154..552
                     /gene="yafO"
                     /locus_tag="IT904_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAP3192601.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHFVYCQGAFDEQAWLLIA
                     ILKPEPHKQARDNNQMHKIGKMAEAFRMRF"
     gene            562..1014
                     /locus_tag="IT904_002846"
     CDS             562..1014
                     /locus_tag="IT904_002846"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP3192602.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMISSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCVERYIDMLFVDPEYTRRGVACALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQY"
     gene            1248..1514
                     /locus_tag="IT904_002847"
     CDS             1248..1514
                     /locus_tag="IT904_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192603.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPTRRRCNGAGNRNGPLL"
     gene            1483..1983
                     /locus_tag="IT904_002848"
                     /pseudo
     CDS             1483..1983
                     /locus_tag="IT904_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2040..3497)
                     /gene="pepD"
                     /locus_tag="IT904_002849"
     CDS             complement(2040..3497)
                     /gene="pepD"
                     /locus_tag="IT904_002849"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP3192604.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHQVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            3758..4216
                     /gene="gpt"
                     /locus_tag="IT904_002850"
     CDS             3758..4216
                     /gene="gpt"
                     /locus_tag="IT904_002850"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP3192605.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4308..5552
                     /gene="frsA"
                     /locus_tag="IT904_002851"
     CDS             4308..5552
                     /gene="frsA"
                     /locus_tag="IT904_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP3192606.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            5610..6011
                     /locus_tag="IT904_002852"
     CDS             5610..6011
                     /locus_tag="IT904_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP3192607.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6050..7105)
                     /gene="phoE"
                     /locus_tag="IT904_002853"
     CDS             complement(6050..7105)
                     /gene="phoE"
                     /locus_tag="IT904_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP3192608.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            7393..8496
                     /gene="proB"
                     /locus_tag="IT904_002854"
     CDS             7393..8496
                     /gene="proB"
                     /locus_tag="IT904_002854"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP3192609.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8508..9761
                     /gene="proA"
                     /locus_tag="IT904_002855"
     CDS             8508..9761
                     /gene="proA"
                     /locus_tag="IT904_002855"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP3192610.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9876..9951
                     /locus_tag="IT904_002856"
     tRNA            9876..9951
                     /locus_tag="IT904_002856"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9909..9911,aa:Thr,seq:cgt)
     gene            complement(9966..11129)
                     /locus_tag="IT904_002857"
     CDS             complement(9966..11129)
                     /locus_tag="IT904_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP3192611.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(11006..11356)
                     /locus_tag="IT904_002858"
     CDS             complement(11006..11356)
                     /locus_tag="IT904_002858"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3192612.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
     gene            complement(11356..11652)
                     /locus_tag="IT904_002859"
     CDS             complement(11356..11652)
                     /locus_tag="IT904_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192613.1"
                     /translation="MTTFTDKELIKERISSLDVRDDIERRAYEIALLSLEVEPDEREA
                     YELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            complement(11652..12014)
                     /locus_tag="IT904_002860"
     CDS             complement(11652..12014)
                     /locus_tag="IT904_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAP3192614.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(12005..12541)
                     /gene="yfdR"
                     /locus_tag="IT904_002861"
     CDS             complement(12005..12541)
                     /gene="yfdR"
                     /locus_tag="IT904_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAP3192615.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(13218..13514)
                     /locus_tag="IT904_002862"
     CDS             complement(13218..13514)
                     /locus_tag="IT904_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192616.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            complement(13700..14326)
                     /locus_tag="IT904_002863"
     CDS             complement(13700..14326)
                     /locus_tag="IT904_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP3192617.1"
                     /translation="METVGQRIKALRRVTGTSQKELGKFCGVSDVAVGYWEKDINTPG
                     GEALSKLAKFFNTSIDYILYGAEFEGKLVTNMRRVPVISWVQAGQFTECRAAEVFSEV
                     DKWVDTSLKIGDNSFALEVKGDSMTNPNGLPTIPEGATVIVDPDAEPRHGKIVIARLD
                     GTNEATVKKLVIDGPQKFLVPLNPRYPNIPINGNCLIIGVVKGVQYEL"
     gene            14424..14624
                     /locus_tag="IT904_002864"
     CDS             14424..14624
                     /locus_tag="IT904_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAP3192618.1"
                     /translation="MTTDDIESYFGSIEKVAAFFGITTEAVYQWRNRPGQLIPKGRAA
                     EAAYRTCGRLPFKPELYEKSNG"
     gene            14662..15213
                     /locus_tag="IT904_002865"
     CDS             14662..15213
                     /locus_tag="IT904_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192619.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            15389..15568
                     /locus_tag="IT904_002866"
     CDS             15389..15568
                     /locus_tag="IT904_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAP3192620.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            15558..15803
                     /locus_tag="IT904_002867"
                     /pseudo
     CDS             15558..15803
                     /locus_tag="IT904_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3981 a         3882 c         3966 g         4218 t
ORIGIN      
        1 gttaatggac atgcttgctg aacaagagga gaaaaagccc ataaaggcgc gcttccgtcc
       61 aagtgctgca agattagagg aaattacacg ccgagctgaa aaatatctta atgatatgac
      121 ggatgatgat ttcaatgact ttaaggaata aggatgcggg tattcaaaac aaaacttatt
      181 cgcctgcaac ttacagcaga ggaacttgat gcgttaacgg cggattttat ttcctataag
      241 cgtgacggtg ttttgccaga tatatttggt cgcgatgcac tctacgacga ctcgtttacc
      301 tggccattaa tcaaatttga gcgagttgct catattcatc tggcaaatgt gaataatcca
      361 tttccgccac agttgcgcca attcagcaga acgaatgacg aagcgcattt tgtatattgt
      421 cagggtgcgt ttgatgagca agcatggttg ctcattgcca ttctgaaacc tgaacctcat
      481 aaacaggctc gagataacaa ccaaatgcat aaaattggga aaatggcaga agcgtttcgc
      541 atgcgttttt gaatttatat tatgaataac atacaaataa gagactatca gcctggcgat
      601 tttcagcaac tatgcgctat tttcattaga gcggttacga tgatctccag tcagcattat
      661 tcaccacaac aaattgccgc ctgggcgcag attgacgaat ctcgctggaa ggagaaactc
      721 gcgaaatcac aagtgcgggt tgccgtcatt aatgcacaac cggttggttt tattacctgc
      781 gttgaacgtt atatcgatat gttatttgtt gaccctgaat acacccgccg cggggttgcc
      841 tgcgctttgt taaaaccttt gattaagtct gaatccgaac ttacggtgga cgccagcata
      901 accgcaaaac ccttttttga acgttatggt tttcagacag ttaagcagca gcgcgttgaa
      961 tgccggggaa cgtggtttac taatttttat atgcgatata aaccgcaata ttaaatccag
     1021 cttgcaatga aaataacgcc cgcctggtat gtgcgcgttt tcttattcca caaactgcaa
     1081 ggaggtaaat catgacaaat cctttatcaa tgactctttg cagacctttc caggattaat
     1141 tctttttttc ttgccctgga ttcgtctgcc atttcctgat ttttatattt atatactcta
     1201 aataattcga gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatggtaca
     1261 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
     1321 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
     1381 gcgtagtggg gatgccagat ttacaccccg gacgcggcta cccgattggc gcagcgttct
     1441 tctctgttgg tcgtttttac ccgacaagac gtcgctgtaa cggtgctgga aacagaaacg
     1501 ggccgctact ctgacacgct gcgttcggcg ctggtttctc tggatggcga taacgcatgg
     1561 gcattaagcg aaagctggtg tggcactatt cagtggattt gtccgagtcc gtatcggcct
     1621 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
     1681 taatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1741 aataaaaccg actcggcggt acgcgccacg catctggcat ccggtattag cgtgaaggtt
     1801 cagtcagagc gcagccagca tgctaacaaa cggctggcgc gattgctgat tgcctggaag
     1861 ctggagcagc agcaacagga aaatagcgcg gcgctgaaat cgcagcggcg aatgttccat
     1921 caccagattg aacgtggcaa cccgcgacgg acatttacag ggatggcttt tatcgaagga
     1981 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     2041 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     2101 ttgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     2161 atgtccattt ccggatacgg ttttttaaac agaccacatt ccaggcccgc gtggataatc
     2221 tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtacctg atgcatcacc
     2281 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     2341 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt
     2401 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
     2461 agggaggttt cgaccacacc ttttgccacg tcggaattgc ggatcacacc gttcggggtg
     2521 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagagcagc tttatcgttc
     2581 gctacagagt ccagcaacaa ggccagattc ttctctttct ctgccagctc gtttttcagg
     2641 atgtcctgat aggtattcac cagagatttc agggcgtcga ctttatcagc tgcgacagca
     2701 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccaccgtt gaaatcgata
     2761 aggcgcaggt ccagctcttc cgcatgaccc gccaggaagc gcaccagcag tttgttggca
     2821 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc cttttagacc ttttaaggtt
     2881 aacttgaagg tttcaaagcc agctggaacc gcttcacgat ccaaatgcag gttggaggtg
     2941 aagtcgatac cccccgcgca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     3001 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     3061 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     3121 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     3181 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     3241 tcgttatttt tctgcggcac catatcgagg tgggcctgca agacgaccgg tttacgattt
     3301 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     3361 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     3421 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     3481 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     3541 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     3601 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     3661 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3721 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3781 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3841 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3901 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3961 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     4021 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     4081 ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg gttgatgatt atgttgttga
     4141 tatcccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     4201 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     4261 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     4321 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     4381 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     4441 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     4501 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     4561 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccacccagg
     4621 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     4681 ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc
     4741 tggccgagca agcgcaggct ttgtcaaacc gcgcctatga agaggccgcc cagcgtctgc
     4801 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4861 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4921 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4981 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
     5041 ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
     5101 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatacc
     5161 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     5221 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gccagtcgtt
     5281 tggggatgca tgatgcttcc gatgaagcgt tgcgcgtgga gctgaatcgc tattcattaa
     5341 aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     5401 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     5461 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     5521 tcacagactg gatcgaaaaa cgtttgtgtt aaaaatttgc taaattttgc caatttggta
     5581 aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac ccgaagagca
     5641 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     5701 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5761 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5821 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5881 aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc
     5941 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     6001 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     6061 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     6121 gttggttgat tttataatct acaaacgctg acatattttt gttgaaataa tacgtagccc
     6181 cgacatcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     6241 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     6301 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     6361 aataaaaagt tgccagataa atattattgg catcgtattt cagacctgta gcccatgctt
     6421 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     6481 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     6541 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     6601 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     6661 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga gtcgccacca aattccggga
     6721 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6781 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc tgtgcagtat
     6841 cactctctgc tttattaccg gcaaactctg cttcccaacg accataacca gtcagttgat
     6901 cgttaatttg tgtttcgcct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6961 tgtcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     7021 cgtctttgtt atatatttct gcggcctgta cggatgcaga tgccacaatg cccatcacca
     7081 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     7141 agcgggagag tcccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     7201 acagattaat aatattctta aaatgtggta atttagtaaa tctgtaataa aagcgtaaac
     7261 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     7321 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     7381 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     7441 acaggcggat cgcgccgtct gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg
     7501 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     7561 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggcg
     7621 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     7681 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgagaacg cttcctgaac
     7741 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7801 gatgctgtcg ctacggcaga gattaaggtc ggcgataacg ataacctttc tgcgctggcg
     7861 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7921 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7981 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     8041 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     8101 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     8161 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     8221 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     8281 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     8341 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     8401 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     8461 gttgccgttc accgtgatga catgattacc cgttaaggag caggctgatg ctggaacaaa
     8521 tgggcattgc cgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     8581 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     8641 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     8701 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8761 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggtctgcgtc
     8821 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8881 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8941 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     9001 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     9061 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     9121 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     9181 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     9241 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     9301 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     9361 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     9421 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     9481 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     9541 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     9601 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     9661 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9721 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9781 aagtagccat ctgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9841 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9901 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc atttaaatca
     9961 ataagttaca catcattagt accttcctta ttttttgact gggacaaatt tgggaccgat
    10021 gggttcagga tcgagtctat ttgccgtgcg tgttcggtaa ggtgattagg tgcaaggtga
    10081 gcatatcgac gaaccatttc gatagactcc cagcctccca tttcctgtaa cactgacaac
    10141 gggactccgg cttgaaccag ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa
    10201 tcatcaatac cagcccgtct cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc
    10261 ttccttactg ttggcgcttt cgttccgtct ggtttggtac agctttcctt gtacacaaat
    10321 acccaacggt gatgattccc gatttgtttt ttcaaaacgc gacatgcagt atcattcagc
    10381 gcaacgccaa ttgcgcggtt tgatttactc tcttccgggt ttatccatgc cacccggcgc
    10441 tgcatgtcta tttgttgcca ttcaaggttg atgatgttcg agcgtcttaa gcctgttgcc
    10501 agtgcaaatt caacaacaga ctttaatggc tccggacatt catcaatcag cctttgtgct
    10561 tcatggggct ccagccagcg gatccgttta ttctttggtt gaggcacttt aataattggt
    10621 gccttatcca gcattttcca ttcacgctct gcggctctta gtagggcctt tataaatgaa
    10681 agatgcgtag ccttcgttgc aacggacgct ggttttggcg tgtattctgg aacaggtttc
    10741 cctttttttc tgcatgcttc tgccctgagt ctccagtttt cctcatgacg ccggttcgtc
    10801 attttctgca ttgctgaata aatttttgat tcagtaatgt ctcttagttg cattcctgcg
    10861 aaatgttgaa gccagaatcc gatccggctt ttgtcatcgt ccagtgattt tttatgtgct
    10921 ttctcttcaa gccacctgac acacgcttcc tcgaacgtta tatcaggtat ttcaccaagt
    10981 ttgctgaccc gccatgcttc agcctttagc ttgtcatgga gttctgtcgc ctgccttttg
    11041 tcctttgttc caagagactg tttaaatctt ttaccgttcg gcaatgtgaa actggcgtac
    11101 catatttcac ctctgcggaa gagtgacatt ttctttcctc tgttatgcca tcacccgcgc
    11161 tcacctggac agtatgcagc ggagactgaa gagccgcaat gcaggcttgt cgtgttgtga
    11221 ggtaaggaga tttattctta gtgggatctt tgcgtgttgc ctgaagacgc cctgtgcgta
    11281 tccagttaat ggcagtcggt ctggatatct tgagaaaatg acaggcctca tcgagtgtga
    11341 ggctgtatgg ctccattatt tcacctcttg ctgtgacatt gttgaaaaat ggataccagc
    11401 tcgttgctgc cagacgatcc aaccgagagt catatcccat gccatgtatt cgttatcgcc
    11461 gttttttgct ctccgacgat ctactaagtc accgaaacgc ttttccatga ataattcata
    11521 agcttcgcgt tcatctggtt ctacttccag agataggagt gcgatttcat aagcacggcg
    11581 ctcaatatcg tctcgcacgt caaggctgct gatacgctct ttaatcagtt ctttgtcggt
    11641 aaaagtggtc attatgctcc agcctccggt gcttttggca ttactgccca gtgagtgata
    11701 ttgacgtttt caaggtcccc gacctgaaat gtccactgcc attctccggt ttctttttgt
    11761 ccccaggtgt accagagaga acgccagcca attagccagc cttctccgtt agcatcgaat
    11821 aacaaaacac tttcatttgc tggtggcagt tcagttgaca ctggtattac tttgttttcc
    11881 tgtgctgcac atttagcttc aagcgcatcg aatttacgca ccaggtattc agcatctgtt
    11941 tcatttactt tcagatctcg cggtacacat ctcccacgaa gaaacccttc catttcgaaa
    12001 acattcatgc gcatttgcgt aactccgata actcgttaaa gcgttccata aacatcccgt
    12061 aggcatggcc cggtgccagt ggaatcacgt tgaacatctc tgttgccggg ataccttcca
    12121 gtacaggcca gaaagagcca tcatcaagcc cgagatcgcg gcgttcggtt gccagcatga
    12181 tgagatcggc atatttcacg ggcgtactca taactggggg taatccgtat ttctcacgga
    12241 ttacggagtc tattttttct tccatttgtt tatagtcagg aagaaggcgt ttcagtggtg
    12301 cgggaatgtc ctggcaatac gcttctgttg catcatgcat taacgcttca aaagcaaatt
    12361 cctgcggcac cagctggctg caaagaaccg catgttgggc gacgctgtag aagtgcgaaa
    12421 gatgaccggc aaagcgacag atatttgaaa gggaaaccgc gatatcgtta atatcgatgt
    12481 cgtctttatt tatcctgtca taataaaaat gcttcccgga aaaagtttta ataaatgaca
    12541 ttttgttctc cacgtatatg cgctgcaccg cgctgaattc gggtaaaagg aagccctcac
    12601 cgtccggcga ttattgagtc aattacattt ccataaatgc ccccgtaggg gcggttagtt
    12661 tctccacaaa acagagaaga acacctgcgg tggcagccgc ccggatggat tgggttatga
    12721 gcccgtcgtc cggtgatgct cttctctgtt ttgtaaaaag gacggtacca gccggaagca
    12781 agggtacaag ctggtaccgc caagactaca cacagcataa agttgtggtg ccgggagcct
    12841 cccggtgcct ggcgaaggtt gcacaccagg cgggtgggta tccacagaag gtcgactgtc
    12901 agcctcaacc ttaacccgcg tgcgctgagc cgcattcacc acaacgctaa ggattctctc
    12961 tggttgaaaa tacttagctg ttatgtgcct gtcttttcac cacttcaggc tcggtggtat
    13021 ccttttaagc gcgtatacat aaaaggaaaa tcaaatgact tttgatgaaa aagaacttga
    13081 taatgcaatt aataaaatca tcgtaacgtc gctcttttcc tgtctcagcg acactcagca
    13141 gaaacagttc tacgaatcgg ctttcaacat gatcgagcgt tgttgtttct gcgatgccga
    13201 cgagttacct gaaaaaatca ggaaacagtt ggctgatgct cttcgagtgc gactttctga
    13261 ccaattttct gaaatgtgct ctccgaattt ggacaaatag aaaaaggcca tttccattca
    13321 gggtctgatg gaaatacttc agcctgttcc aaagcacggc gtaaagagaa cacaactcca
    13381 gccataatct gatgtttccc attggtccag ctatcgccgc tctgatctac aggggcggct
    13441 atgtcgtatg accaaacgac ttcacagtta ttgtttaaaa tctggacttt catttcatac
    13501 acctgcttta acatgagtgc ctggtggcac aacatgactc aacgaatcat cctggacttc
    13561 atatgcccca ggcggctact tcgtgggcgt cctgcctgtt cgttatcttt gatataaaat
    13621 ctaacttaac ttagttatta tggcaagaga aaacaccaaa cttttcttag ttcggtgcct
    13681 tagttagaga agagaggtct tagagttcgt attgaactcc tttgactaca ccaatgataa
    13741 ggcaattacc attgataggg atgttgggat accgaggatt taatggcact aaaaactttt
    13801 gagggccatc gatgactaat ttttttactg tagcttcgtt tgttccatca agtcgagcga
    13861 tgactatttt tccatgacga ggttctgcat ctggatctac aatcactgtt gcgccttctg
    13921 gtattgttgg gaggccatta gggttagtca tggagtcacc tttaacctct aatgcaaatg
    13981 agttatcacc aatctttaat gatgtatcta cccacttgtc cacttcacta aacacttctg
    14041 ctgccctgca ctcagtaaac tgcccagcct gaacccacga tattacagga actctgcgca
    14101 tgtttgtgac gagtttgcct tcaaactcag caccataaag aatgtaatct attgacgtat
    14161 tgaagaactt cgctaatttc gaaagtgcct ccccaccagg ggtattgatg tctttctccc
    14221 agtaccccac agcaacgtcg cttactccac aaaatttacc caattctttc tgggacgttc
    14281 cggtaactct tctcagagct tttatacgct gaccaaccgt ttccatagga gcaccatttc
    14341 ttgaattgct aagtaatctt agtttttatt gaccaaagat agatttgtaa ttagcatcta
    14401 ataaaactta gtttggaggg cgtatgacaa ctgacgatat cgaaagctac ttcggcagta
    14461 ttgagaaagt tgctgctttt ttcggcataa caactgaagc cgtttatcag tggcgaaacc
    14521 gtccgggcca gttaattcca aaaggacgtg cagcagaagc tgcatataga acttgcggac
    14581 ggttgccatt taaacctgag ctttatgaaa aatctaatgg ataaatcgat taacagaaac
    14641 cacagaacga tgaggctaac cgtgggtaag catcactgga aagtagaaaa acagcctgag
    14701 tggtacgtga aagctgtcag aaaaactatc gcagcgttgc cgggtggtta cgctgaagca
    14761 gctgactggc tggatgtaac agaaaacgca ttatttaacc gccttcgtgc cgatggcgat
    14821 cagattttcc cgctgggatg ggcaatgatt ttgcaacgtg ctggtggaac tcacttcatt
    14881 gctgacgctg tggcgcagtc tgcaaatggc gtctttgtgt ctcttcctga cgtcgaggat
    14941 gtggacaacg ccgatattaa ccagcgcctg ctggaagtca ttgaacagat tggcagttat
    15001 tccagacaga ttcgttcggc aatcgaagac ggtgtggtgg aaccgcatga gaagacagca
    15061 attaacgacg agctgtatct ctcaatttcg aagctgcagg agcatgcagc actggtctac
    15121 aaaatctttt gcatttcaga aagtaatgac gcccgcgagt gtgcagctcc gggcgccgtg
    15181 gcgtgtcgtg actgtggaga aactaacgca tgaacagttt aacaacacac taccgtcgct
    15241 cgcaactgat tgcgcttcct gtaccgggtg gaaaagcgaa ggtggagtat tgctatgcag
    15301 ttaatgtacc aggtgacagg gaaattgtaa cccacagctt tgctgagtgg gctgtgggtg
    15361 atttcaaccg gcagaaggag acagtccttt gcgacaagtt aaccgctggt tcaaagatca
    15421 ctacggagtg cccgtcagag tcattcgttg ggagccggaa acacaacgag ttatctacct
    15481 ccgcgaaggc tatgagcatg agtgcttcag cccgctcgaa cagtttcgtc gtaaattcag
    15541 ggaaatagag gtcggtcatg agcactaaat taaccggcta tgtatgggat ggttgcgctg
    15601 catcaggcat gaaattatcc agcgtggcaa ttatggcccg cctggctgat ttcagtaatg
    15661 acgaaggtgt gtgctggcca tcaattgaaa ctattgcccg tcagattggc gcggggatga
    15721 gtaccgtcag gacggctatc gcacggctgg aagcagaagg ctggttaacg cgtaaggcgc
    15781 gtcgccaggg taaccgcaat gcgtcgaatg tttatcagct taacgttgcg aagcttcagg
    15841 cagcggcatt ttctcaactg tcagattctg acccgtcaaa atctgacgca tcaaaatctg
    15901 acccgtcaaa atttgatgcg tcgaaatctg gcaaaaaagc gggttttcac ccgtcagaat
    15961 ctggcgggga tccgtcagta aaatcaaaac atgatccgtc agataaaaaa ccttctcgtc
    16021 cggacgcttc gcaaccggac acgcaga
//
LOCUS       DACAPB010000101        16043 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.101, whole genome shotgun sequence.
ACCESSION   DACAPB010000101 DACAPB010000000
VERSION     DACAPB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.101"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(60..395)
                     /locus_tag="IT904_002868"
     CDS             complement(60..395)
                     /locus_tag="IT904_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAP3192621.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            796..3024
                     /gene="crfC"
                     /locus_tag="IT904_002869"
     CDS             796..3024
                     /gene="crfC"
                     /locus_tag="IT904_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAP3192622.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHICHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            3021..3899
                     /locus_tag="IT904_002870"
     CDS             3021..3899
                     /locus_tag="IT904_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192623.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            4163..5665
                     /gene="proP"
                     /locus_tag="IT904_002871"
     CDS             4163..5665
                     /gene="proP"
                     /locus_tag="IT904_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAP3192624.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5777..5866
                     /gene="pmrR"
                     /locus_tag="IT904_002872"
     CDS             5777..5866
                     /gene="pmrR"
                     /locus_tag="IT904_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAP3192625.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5842..6942)
                     /gene="pmrB"
                     /locus_tag="IT904_002873"
     CDS             complement(5842..6942)
                     /gene="pmrB"
                     /locus_tag="IT904_002873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAP3192626.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6943..7611)
                     /gene="basR"
                     /locus_tag="IT904_002874"
     CDS             complement(6943..7611)
                     /gene="basR"
                     /locus_tag="IT904_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAP3192627.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(7608..9251)
                     /gene="eptA"
                     /locus_tag="IT904_002875"
     CDS             complement(7608..9251)
                     /gene="eptA"
                     /locus_tag="IT904_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAP3192628.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RMPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(9355..10692)
                     /gene="adiC"
                     /locus_tag="IT904_002876"
     CDS             complement(9355..10692)
                     /gene="adiC"
                     /locus_tag="IT904_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAP3192629.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10829..11590)
                     /locus_tag="IT904_002877"
     CDS             complement(10829..11590)
                     /locus_tag="IT904_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP3192630.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFVSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11915..14182)
                     /gene="adiA"
                     /locus_tag="IT904_002878"
     CDS             complement(11915..14182)
                     /gene="adiA"
                     /locus_tag="IT904_002878"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAP3192631.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(14381..15289)
                     /gene="melR"
                     /locus_tag="IT904_002879"
     CDS             complement(14381..15289)
                     /gene="melR"
                     /locus_tag="IT904_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAP3192632.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            15572..>16043
                     /gene="melA"
                     /locus_tag="IT904_002880"
     CDS             15572..>16043
                     /gene="melA"
                     /locus_tag="IT904_002880"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP3192633.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNT"
BASE COUNT         3887 a         3871 c         4157 g         4128 t
ORIGIN      
        1 ctggcactgt tgccggatgc ggcgtaaacg ccttatccgg cttaccgatc acaatacaat
       61 cagttctttt tcacaaactc agatttcagt ttcatcggac caaaaccgtc gattttgcaa
      121 tcgatgttat ggtcgccttc aaccaggcgg atgtttttca ctttggtgcc aattttcagc
      181 atcgaagagc tacctttcac cttcagatct ttaatgatgg taacgctgtc gccgtcagcc
      241 agcagattgc cgttagcatc tttaacgatc agctcgtcgc tttcctgtgc aggttctgcg
      301 tcgttccatt cgtaggcaca ttccgggcag atgtacatgc cgttatcttc gtaggtgtat
      361 tcggagttgc attttgggca gtgtggtaat gacatgtgga tttcctcaaa agtcagcagg
      421 gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg caattataca caaaatccct
      481 gagtttgtcg ggggtactgc tgattttaga cgacgatcgt tttcatttga aaggaaacgc
      541 aaaaagaaaa taatcttttt gcctctattt attatttaag ataatgttaa tgctctactt
      601 gttctttttt taattcacat gatattttta tctttctgac gttttaaacg tatcagaaga
      661 cgttatttat tctgataaaa aacaacacac taaatataaa attatttctt gcagtaatta
      721 taaaagccga aaacagaact caaaaacaat ctggctaaat aaaataacaa aatttgcttt
      781 aaggaagaat tttctatgta cacacagacc ctgtatgagt taagtcagga ggctgaacgc
      841 ctgttacagc tttctcgcca acagttgcag ttactggaaa aaatgcctct ctctgtaccc
      901 ggagacgacg cgccacaact ggctttaccc tggagtcagc ctaatatcgc cgaacgtcac
      961 gcgatgctga ataatgagtt gcgtaaaatt tcccgactgg aaatggtgct tgcaattgtc
     1021 ggtaccatga aagcagggaa atcaaccacc attaatgcca ttgttggtac ggaggttctg
     1081 cctaatcgta atcgcccaat gactgcgctg ccgacgctta ttcgccatac gcccgggcaa
     1141 aaagaaccgg tactgcattt ttcacatgtc gcgccaatcg accgtttaat tcaacaatta
     1201 caacagcgcc tgcgtgattg cgatattaag catctgaccg atgtgctgga aatagataaa
     1261 gatatgcgtg cgcttatgca gcggatcgaa aatggcgtcg ctttcgaaaa atattatctg
     1321 ggtgcccagc ctatttttca ttgtctgaaa agtttgaatg atttagtgcg actggcgaag
     1381 gcgctggacg tcgattttcc tttttctgct tacgccgcca ttgagcatat tcccgtgatt
     1441 gaagtggagt ttgtccatct ggcggggctg gagagttatc ccggtcagtt gacgctactg
     1501 gatacccccg ggccaaatga agccgggcaa ccgcatctgc aaaaaatgct taaccagcag
     1561 ctggcacgcg cctcggcggt actggcggtg ctggattata cgcaactgaa atcgatctcc
     1621 gatgaagagg tccgtgaggc gattttggcg gtggggcaat cggtgccgct gtatgtgctg
     1681 gtcaataagt tcgatcaaca ggatcgtaac agtgacgacg ccgaccaggt gcgggcactg
     1741 atttccggga cgctgatgaa aggctgtatt acgccacagc agatatttcc ggtgtcgtcg
     1801 atgtggggct acctggcgaa tcgggcgcgc catgagttag ccaacaacgg taagttaccc
     1861 gcgccagagc aacaacgctg ggtggaagat tttgcccatg ccgcgctcgg caggcgctgg
     1921 cgtcatgccg atctggcgga cctcgaacat atttgtcatg ctgccgatca gttgtgggaa
     1981 gattcgctgt tcgcccagcc aattcaggcg ttgcttcatg ccgcttacgc taacgcctcg
     2041 ttgtatgctc tgcgatctgc tgcgcataaa ctgttgaatt acgcgcagca ggcgcgggaa
     2101 tacctggatt ttcgtgcgca cgggttaaac gtcgcttgtg aacaattgcg gcaaaatatc
     2161 catcaggtcg aagaaagttt gcagctattg caactcaatc aggcgcaggt gagcggcgag
     2221 attaaacatg aaatcgagct ggccctgacc tccgccaacc actttctgcg tcaacaacaa
     2281 gatgcgctga atgcgcagtt agccgccttg tttcaggatg attcggagcc gttaagcgag
     2341 attcgtaccc gctgtgagac actgttacag acggcgcaga acaccatcag tcgcgacttt
     2401 acgctgcgtt ttgccgagct tgaatccacc ctttgccggg tgttaaccga tgttattcgc
     2461 cccattgagc aacaagtcaa aatggaattg agcgagtcag ggtttcgtcc tgggtttcat
     2521 tttcctgttt ttcacggcgt agttccccac ttcaacactc gccagctgtt cagtgaagtc
     2581 atttcgcgcc aggaagcaac ggacgagcag agcacgcgat taggcgttgt gcgtgagact
     2641 ttttcgcgct ggttgaatca gcctgactgg ggacggggaa atgagaaatc cccgacagaa
     2701 acggttgatt acagtgtgtt gcaacgagca ttaagcgcag aagtcgatct ttattgccaa
     2761 caaatggcta aagttctggc agagcaggtc gatgaatctg ttacggcagg catgaatact
     2821 tttttcgctg agttcgcttc atgtttgacg gaattacaga cgcgtttacg cgaaagtctg
     2881 gctctgcgtc aacaaaatga atcggtggtc aggctgatgc agcagcaatt gcagcagact
     2941 gtgatgactc acggctggat ttacaccgac gcccagctgt tacgcgatga tattcaaaca
     3001 cttttcacgg cagaacgata ttgaccaaga cgttacttga cggccccggt cgcgtgctgg
     3061 agtcggttta tccccgcttt ttagtggatc tggcgcaggg cgatgatgcc cgccttccac
     3121 aagcccatca gcagcagttt cgtgaacgac tgatgcagga acttcttgcg cgcgtgcagc
     3181 ttcagacatg gacgaacggc ggcatgttaa atgcgccgct tagcctgcgt ctgacattgg
     3241 tggaaaaact ggcgtcgatg ctggatcccg gtcatctggc actgacgcag atcgcgcagc
     3301 atctggcgct gctgcaaaaa atggatcacc gccagcactc tgctttcccg gagctccccc
     3361 agcaaattgc cgccttgtat gagtggtttt cagcccgttg tcgctggaag gaaaaggcat
     3421 taacgcaacg aggcctactg gtgcaggcgg gtgaacagag cgagcaaatt tttacccgct
     3481 ggcgtgctgg ggcgtataac gcctggtcgt tgcctgggcg ctgttttatc gttctcgaag
     3541 agttgcgttg gggggcattt ggcgatgcct gtcgtctggg aagcccgcaa gcggtggcgt
     3601 tgttgctggg tgatttgcgc gtgaaagcga cacaacatct ggcagagagt atcaatgcgg
     3661 ccccgaccac gcgtcactat taccatcagt ggtttgcctc ttcgaccgtt ccgacgggcg
     3721 gggatcatgc tgatttttta agttggctgg gaaagtggac cacggcagat aaacaacccg
     3781 tttgctggtc agtgacccaa cgttggcaaa ctgtcgcgct ggggatgcca cgactctgtt
     3841 cagcgcagcg tttggtgggg gcaatggtcg aggaaatctt ctctgtaaat ttggcgtaaa
     3901 taatcagtta catcaatgag tcctaaacga aatccatgtg tgaagttgat cacaaattta
     3961 aacactggta gggtaaaaag gtcattaact gcccaattca ggcgtcaact ggtttgattg
     4021 tacattcctt aaccggaggg tgtaagcaaa cccgctacgc ttgttacaga gattgcatcc
     4081 tgcaattccc gctccccttt tgcggccgtc gcgctgattt ttctggcgtt tgcggaaatg
     4141 ggccaactct gcgaggaaag ctatgctgaa aaggaaaaaa gtaaaaccga ttacccttcg
     4201 tgatgtcacc attattgatg acggtaaact gcgtaaagcc attaccgcag catcactggg
     4261 taatgcaatg gaatggttcg attttggtgt ttatggtttt gttgcttacg cattaggtaa
     4321 agtttttttc ccgggggctg accccagcgt gcagatggtt gctgcacttg ccactttctc
     4381 cgttcccttt ctgattcgac cgcttggcgg actcttcttt ggtatgttgg gcgataaata
     4441 tggtcgccag aagatcctcg ctatcactat tgtgattatg tcgatcagta cgttctgtat
     4501 tggcttaata ccgtcctacg acacgattgg tatttgggca ccgattctgc tgttgatctg
     4561 taagatggca caaggtttct cggtcggcgg tgaatatacc ggggcgtcga tatttgttgc
     4621 ggaatactcc cctgaccgta aacgtggctt tatgggcagc tggctggact tcggttctat
     4681 tgccgggttt gtgctgggtg cgggcgtggt ggtgttaatt tcgaccattg tcggcgaagc
     4741 gaacttcctc gactggggct ggcgtattcc gttctttatt gctctgccgt tagggattat
     4801 cgggctttac ctgcgccatg cgctggaaga aactccggcg ttccagcagc atgttgataa
     4861 actggaacag ggcgaccgcg aaggtttgca ggatggcccg aaagtctcgt ttaaagagat
     4921 tgccactaaa tactggcgca gcctgttgac atgtattggt ctggtaattg ccaccaacgt
     4981 gacttactac atgttgctga cctatatgcc gagttatttg tcgcataact tgcattactc
     5041 cgaagaccac ggggtgctga ttattatcgc cattatgatc ggtatgctgt ttgtccagcc
     5101 ggtgatgggc ttgctgagtg accgttttgg ccgtcgtccg tttgtgctac ttggtagtgt
     5161 tgccctgttt gtgttggcga tcccggcgtt tattctgatt aacagtaacg tcatcggcct
     5221 gatttttgcc gggttactga tgctggcggt gatccttaac tgctttacgg gcgttatggc
     5281 ttctaccttg ccagcgatgt tcccgacgca tatccgttac agcgcgctgg cggcggcatt
     5341 taatatttcg gtgctggttg ccggtctgac gccaacactg gcggcctggc tggtcgaaag
     5401 ctcgcagaat ctgatgatgc ctgcctatta cctgatggta gtggcggtga ttggtttaat
     5461 caccggcgta accatgaaag agacggcaaa tcgtccgttg aaaggtgcga caccggcggc
     5521 gtcagatata caggaagcga aggaaattct cgtcgagcat tacgataata tcgagcagaa
     5581 aatcgataat attgaccacg agattgccga tttgcaggcg aaacgtaccc gcctggtgca
     5641 gcaacatccg cgaattgatg aataagctga aacggatggc ctgatgtgac gctgtcttat
     5701 caggccaatt gaactcttaa ggttcactta atctctgacg cgcatactct cctccaggtt
     5761 aacggaggag agtgcaatga aaaaccgtgt ttatgaaagt ttaactaccg tgttcagcgt
     5821 gctggtggtc agcagctttc tttatatctg gtttgccacg tactgatctt tcttcagccg
     5881 tacccaggcc cgcgtgccgg aagtctcttg ccggttttgc aggaaaaact gcccgtgatg
     5941 caactgtgta atgcggctga caatacttaa ccccagacca atcccgccat aacggctgtc
     6001 catacgtaca aacgctttac tcaactcccc gcatttactc tcatcaatac ctggtccttc
     6061 atcttcaact gccatgaccg ctccgtcatc ttcttgcagc ttaatcataa tgttgctgcc
     6121 ttgcgggctg taacgatggg cgttttctac caggtttcgc aataacatcc gcagcagggt
     6181 tgcatcaccc tgaacggtga tgtcggcggc gctctctggc aatagcaggg tttgctgtcg
     6241 ctggtcgagc atggtactga gttcgtcata cgaggggaga atgacatctt ccagcagttt
     6301 tacatgttga taattaccgg aagaaaatga ctgtccggca cgcgccagtt gcagcagctg
     6361 ggagacgctc tccatcatct gatcaagccg tgccactaac ggtgctacat caatgtgatg
     6421 cgttttcgcc agcagttcca gatgcaaacg cacccccgcc agtggcgttc gcagttcgtg
     6481 cgcgacgtca gcggtaaaca acctttcgtt atccagcgtg ctggtcaggc gactgaccag
     6541 atcgtttaac gccgaaacca ccgcttcgat ttcgagggtg gcgctgtgaa tggcaatggg
     6601 cgttaagttg tcggcggtgc gcgcttccag ctctttttgc agctccgcca gcgggcgggt
     6661 gatgcggcgt accgcctgat agcagataaa tagcgtcagg ctgaccataa agacgccggg
     6721 gacaatcagg ctggcgaccg cctcgcggat ctcacgcatg atgtggcgat cgttgttgcg
     6781 attgtcgcgt agcgcctgct caaacagctg aatctgctcg gtactttcat gccatagcca
     6841 gaagacgctg atcagctcaa acaccaacaa aatggccccg atggtcaata tcagccgttg
     6901 gcgcagcgat attggtcggc gcagaaaacg catcagattc aattagtttt cctcattcgc
     6961 gaccagcata tagccaaagc cgcgcacggt gcggatacgg gctttgccca ctttgtcgcg
     7021 cagattgtgg atatgcactt ccagggtgtt ggtcgagggt tcattgtccc agttatagat
     7081 gtcgttgtag agaatttccc gatgcaccgg actgcctgct ttgagcatta accgtgacag
     7141 cagagcatat tctttgggcg tcagaatcaa ctcttcaccg cccatccata cctgacggcg
     7201 acccatattc agcgtcagat tgccaacaat cagctcactt tcgccctgat tattatggcg
     7261 tcgtagcagg gctcggatac gggcgtgtaa ctcttccagc gcaaagggct tcaccagata
     7321 gtcatcggca ccgacatcca gcccggcgat tttgtcggtc agcgtatcgc gagcggtgag
     7381 gatcagtacc ggcagggtgt attttttctg ccggatacgg gcgagaaaat gcagtccatc
     7441 ttcgtcgggt aaccctaaat ccagtaccac caggctgtaa tgaccggcct caaggctttg
     7501 ttccgccatc cgcgcggttg tcacgccatc gcacgcgtag ccttcggttt gcgccgccag
     7561 aatcagtccc tgcaataaca gcgtatcgtc ttcaacaatc agaattttca tttactcact
     7621 ctcctgcaag tttgcagaat atcatccgca gcctggtaat acttcgtctc aacgccagtt
     7681 aatcccaata gcgtggagaa taaattgtct tgtgaatagt gttgcgtttg cgcctgtttt
     7741 tgcaggcagt tctggtcaac ctgataccgt ttttgataat cctccgacag ccacagcagc
     7801 attggcacct gtttttggct atccggggcg atggcataag gcagaccgtg cagatagatg
     7861 ccattttcac ctaacgattc accgtggtca gaaagataaa ccaggctggt ggtaaattta
     7921 tcctgatgtt ctttaagcag attaatcgct ttatcaacaa tatagtcgac gtaaaccagc
     7981 gtgttgtcgt aagtgttcac cagttgctct ttggtacagg tctggatctc attggtgtcg
     8041 caggttgggg taaatttcct gaactgaggc ggatagcggt tgtaataggt cggaccgtgg
     8101 ctgccgatgg tgtgtaagac aatcacgcca tcaccttgca ggttattgat gtactcttca
     8161 agcccgtgga acagcacttc gtcatagcat tcgccgttga tgcactgatc aggtagattc
     8221 agcgcggtga cgttctggtg aggcatgcgg tcgcaggcac ctttacagcc gccatcgttg
     8281 tcattccaca gcacgttgat gcccgctcgc tgaatgatat ccagcacgcc ttcctggtgc
     8341 tgtgccagct cttctttgta gtgctcacgc ggcatatccg agaacatgca cggtactgaa
     8401 actgccgttg ccgtgccgca agatgcggta ttagggaaat agaccacgtt atctttcgcc
     8461 agccgcgggt tagtttcacg cgggtagccg ttgagggaga agttctccgc ccgcgaggtt
     8521 tcgccgacaa tcaggatggt caaattttta cgtttttcgt tctgcattaa cgggttgcgg
     8581 tgcgcgtctt caccaattcg caccagcggc agatttgcca gtcgctgatg ggagtaccat
     8641 gaccagctgg caacaatgct gttagagggg cttaaggatt tcaccagctc tttgttattg
     8701 cggaacaacg aggcgtagtc tttataaaac agtgcggcga ccagcaaaat cagtagtaca
     8761 gaaaccagaa tattggctcc acggaaaaga acactgcgca gacgcgaggt ggcaggtttg
     8821 atttttatcc agcaggcaat cagcgcagca agcacgccgc tgaatcccag cgttaataac
     8881 atttgcggtg tcatcagcgc ataactttct gccggagtgg tatcaataat attggcaatc
     8941 atcgagcggt cgatgacgat gccgtaagtc attatgaaat attgtgcagc cgcgccaacc
     9001 agaataaaca ggcaggccag tggtcgatta agccataaga aagagcttag tgtcaggaca
     9061 atattaatca cgctgaaagc gacgaccggc atcgacaaga aaaccagtac gttatgcagc
     9121 gaatccagcg gcagcgcctg caacacctgt ttaaaaaagg caatattcag gcagatagag
     9181 atataaaaag cggccaacaa tagccaggcg agtaaattca aagagggtct ttttagtagg
     9241 cgcttcaaca tcacggtgtt tccatcgaac aaagtgcgca tatgctcgca aagcaaaatt
     9301 aagccaacct taagttctta aggttggctt ttatgtttgc tggattaagg ggaattaatc
     9361 tttgcttatt ggtgcatcta agggatacgg gtttttatgt agccggttgt aattcagcgc
     9421 atacatggcg gtgatgacca tcagggtgac aaatgaccac ataacctctt tcgctccgga
     9481 ccccaccacg gcccagatgc agtagaggaa ggcaatggta gtaactgcca gatatgccgg
     9541 gcgtgcttta ccaaagtgac cgtgtccgag cagcagtaac gccgcacagg tgtaaagata
     9601 tggcaccagt gtaaagatga ccgagacgga agaaaccaga ccgaactctt tggtcgcgtt
     9661 tggtgaaatg ctgctgagct ggaagatggt catcaaaata ccgacgataa tcagccccgc
     9721 cactggcgta cccgctttat ttacacgggc aaaaatcggt gggaacagtc cgtcatcggc
     9781 agcggctttc gccgtttgac ccgccagcaa cgtccagccg cccagtgaac ctaagcaacc
     9841 cgcagctgcg cagaaggaaa caatggcccc ggcggtgtca cccaacgcca tccgtgcggc
     9901 atcaccgaat ggcgaagcag aaacgcgcag tgcggcatta gggatcatcc ccataatcgc
     9961 ggtggtagaa agtacatagc aaacggcggc aatcaatacc ccaccaatgg tggcgatagg
    10021 gacattgcgt ttcgggtttt tcaccacacc tgcggcaacg gaggcacttt ccacaccgat
    10081 gaacgaccac agcgtaacgt taagggtact ttgaattgca ccgaaggtgc ccaggccgct
    10141 gacgttccat gccgccatat aggtttcacc acggaaccag aaccagccaa atacggcaat
    10201 cccgacgatg gggatcagcg ccagcacggt ggcaactgcc tgcacacggg tgatcatttt
    10261 cggaccgaca atgttcagca ggacgaagat ccacagcacc acgacgcagg tgatggttaa
    10321 taccaatgga tctttcagaa tcgggaagaa gtaacttaaa tatcctacgc caatgaccac
    10381 catggcgata ttgccgatcc agcaggccag ccagtagagg acgttggttt gataaccgag
    10441 aaacgggcca aagcagcggc gggcgtaagc gtaagaacca ccaggacttg ggtcgaggaa
    10501 cgacattttg gcgtatacca tcgagagccc cagtgcaccg ataatcgtca ccaaccatcc
    10561 ataaatggca atcccgccag tagaggccag gtttgcaggt aacagaaaaa cacctgaccc
    10621 cataatattc cccgacacca tcagggtgac ggggattaag cccactttgt gagcatcagc
    10681 atccgaagac ataattaaac tcctgcgaag gcgagcttcg tgacaataaa ttacgtcata
    10741 tcatacgcct gcattcgata gttgaattat tatcgaccgg gttatcgctg atgccgttat
    10801 taatatagct cttcgttaag gttaaaaatc aggcgacagt gcgttctctg tgctgactaa
    10861 cataatgcag cggcgtcata ccgtagaagt ctttaaagac agaaataaag tacgacgtac
    10921 tgttgtagcc gcaggactgt gatacctgag agatattttt accgtccatc attaattcat
    10981 ttacggcata acgcatgcgg caggtggtga ttatttggct ataactggtg ttttcgcttt
    11041 tcaacttttt ctttaacaga cttgggctaa gacataaaca actggctacc atactaagat
    11101 tccagtcttt gtgaatatcg ctttcaataa tttgataaac gctgtcactt acacaattac
    11161 gtaacatata catcattaat gaaacgaatt ttttactgtc gagaaaacgg gatagcaccg
    11221 tgaataataa tgcgcgtgtt cgttcctttt ctgactcagt ttctgcgggc atcatgctgt
    11281 gttgcgcggc aacgcgaaag acgtctggcg tcaggcatgg cagggtgagt atgggcgtag
    11341 cgctacgttg ccatactggt atttgcgaga gatctttatt cagaaaccgg aggtaatctt
    11401 tgatgatgtc gtgactaata tgagcaacca aagtgttgtt aagagaggag acgtcgataa
    11461 tattattttc acaatttaat aacgccatat ggttagcttt aaggctaata ggttctttcc
    11521 cattcaccct tatccagaca tctttttcag tcaataaaac aatacaaggt tggtcgctgc
    11581 aaatcctcat acatgtactc ctgagtgcga ataaaagacc agttctcgct aaagcaaagc
    11641 gataccggga aatggttata taattgtatg atgaatataa acatgcataa aaataacgag
    11701 ttttactaat agtacaaatt tttatagtaa tttctcatgt acatattttt ttacaggata
    11761 aataacaggc aaaaaaaagc gcggggtggg gccgcgcaaa aagtatgaca tttttgtttg
    11821 cagcaatttt tatgaaaacg ttgccatact caaaaggcct aaggccaggc ggcgtaatgt
    11881 tatttaaaca attacgcctt cagcggaata gtggttacgc tttcacgcac ataacgtggt
    11941 aaataccgtc aataatttca gtcccttcag tttcgtgttc aaatccaggg aaatggtggt
    12001 cccaggattg cagcgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt
    12061 caccagacag cagcatcggg attcctggcg gatacgggat aactgagttt gccgcgatgc
    12121 gtcctggcag attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg
    12181 cttcacgcgg ggtgacttcc gccaccggca ggccggaata ggcttcgttc aaccgtgcgc
    12241 cagggttgtt ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt
    12301 tcgcgtaagt gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt
    12361 tggcgtcata gtggcgtttg aaggagcaaa gggtgttaac cagagttccc catttcccac
    12421 gggttacgcc catagagaac aggaacataa tttggaagtc agtggtgcgg gtaggtacaa
    12481 tgccgtggcg accaagccag gcagtgacca gcgccgccgg aacaccggtt tcttccagtt
    12541 caccatcttc acccattccc ggagcaagga tgctgacttt aatcgggtcg agcatactcc
    12601 agttatccgg aatatctttg aagccgtgcc agctttcgcc cggatgcatt acccagcagt
    12661 cctgaacggt ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt
    12721 gtgggtcggt gacgacttct ttgttccacg gtttgaagaa ccagctaccg tcagcggtga
    12781 actctttata tagccgcgcc atcgcctgac ggaaatcaac cgcttcgtca atcacttcct
    12841 gtgtcagtga caggccgctg ttgccgtcca tcatcgacac cgccacgtcg ttggatgcgc
    12901 agatggcata cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga
    12961 agttaatcgc cccacgacct tcacgtacat gaatataaga agcctgtgac agcgcattca
    13021 gcagtttgtg ggtggagtgg gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc
    13081 cgcgcatggc atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt
    13141 cgtcaaagtg cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat
    13201 aacacacgcc gtcataggtg cagttggtca ccacgcagta agacggtttt tgcccggctt
    13261 tgtctttggt cagcgggctt tcactgattt tcttctgcaa ggtttcaggt tgcatttcct
    13321 gcggatagat tggcccgata atgccgtagc ggttgcggct tggcaccata tagaccggtt
    13381 tcgcgcctgt cagcatcaaa ccttgttcga tggatttatg gcagttacgg tcaacgacca
    13441 cgacatcgtt atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga
    13501 cgactaccga ccaggagcga tcggcaccaa atacgcgtgc ggcatatttt tcgctttcgc
    13561 caaatgcgcc agtatggtca agcaaagaac cgagggaagt tcgttcgatg cccatgtcgg
    13621 tgcggaacag attttcacca tagtagtcat ggtagaaacg tccggcgggt gttttggtaa
    13681 aaccaacgcc gccctggtgg cctggcgctg cccaggaata ttcatggatg tcactatatt
    13741 tcatcagcgc gctgaacagt ggcggcaaca gctgctggcg gtagcgggtc atcgcggcaa
    13801 cggcgcgtcc ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa
    13861 gctccagcag gtcgcgatcc attgcggcga gggctttttc ccgatcgccc aacaggaaga
    13921 ccggcacgtt ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt
    13981 cgtccggatg ttccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga
    14041 gaatggcaaa accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta
    14101 aagcatctgc cagacgctca acggcgttac cgacccaggt gtcttgatgg agaaactcgc
    14161 tttcaacaat taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg
    14221 caagtatctt ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata
    14281 tttagtttta taaacaatac gttatgtgat tattttaaat tattttcact gcttattatc
    14341 gacggctaaa ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc
    14401 ggcgctgttg gctaagtttg cggtattgtt gcggcgacat gccgacatat ttgccgaacg
    14461 tgctgtaaaa acgactactt gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt
    14521 tatcggtatc gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta atgtactgtt
    14581 tcatcgtcaa ttgcatgacc cgctggaata tccccattgc atagttggcg ttaagtttga
    14641 cgtgctcagc cacatcgttg atggtcagcg cctgatcata gttttcggca ataaagccca
    14701 gcatctggct aacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg
    14761 ttttattgac cagaatcggt tcccagccag agaggctaaa tcgcttgagc atcaggccaa
    14821 tttcatcaat ggcgagctgg cgaatttgct cgttcggact gtttaattcc tgctgccagc
    14881 ggcgcacttc aaacgggcta agttgctgtg tggccagtga tttgatcacc atgccgtgag
    14941 tgacgtggtt aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat
    15001 taaaaatcgc catgctctga caggttccgg tatctgttag ttggtgcggt gtacaggccc
    15061 agaacagcgt gatatgaccc tgattgatat tcactttttc attgttgatc aggtattcca
    15121 catcgccatc gaaaggcaca ttcacttcga cctgaccatg ccagtggctg gtgggcatga
    15181 tatgcggtgc gcgaaactca atctccatcc gctggtattc cgaatacagc gacagcgggc
    15241 tgcgggtctg tttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatggctctc
    15301 tttcctggaa tatcagaatt atggcaggag tgagggagga tgactgcgag tgggagcacg
    15361 gttttcaccc tcttcccaga ggggcgaggg gactctccga gtatcatgag gccgaaaact
    15421 ctgcttttca ggtaatttat tcccataaac tcagatttac tgctgcttca cgcaggatct
    15481 gagtttatgg gaatgctcaa cctggaagcc ggaggttttc tgcagattcg cctgccatga
    15541 tgaagttatt caagcaagcc aggagatctg catgatgtct gcacccaaaa ttacatttat
    15601 cggcgctggt tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgcgaggc
    15661 gctgaaaacg gcgcatattg ccctgatgga cattgacccc acccgcctgg aagagtcgca
    15721 tattgtggtg cgtaagctga tggattcagc aggggccagc ggcaaaatca cctgccacac
    15781 ccaacagaaa gaagccttag aggatgccga ttttgtcgtg gtggcatttc agattggcgg
    15841 ttatgaacct tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac
    15901 cattgccgat acgttggggc cgggcggtat tatgcgcgcg ctacgtacca ttccgcatct
    15961 gtggcaaatt tgcgaggaca tgacggaagt ctgccccgat gccaccatgc tcaactatgt
    16021 taacccaatg gcgatgaata cct
//
LOCUS       DACAPB010000102        16002 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.102, whole genome shotgun sequence.
ACCESSION   DACAPB010000102 DACAPB010000000
VERSION     DACAPB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.102"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1077)
                     /locus_tag="IT904_002881"
     CDS             complement(<1..1077)
                     /locus_tag="IT904_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="involved in the transport of lipid A across the
                     inner membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HAP3192634.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPAL"
     gene            complement(1114..3378)
                     /locus_tag="IT904_002882"
     CDS             complement(1114..3378)
                     /locus_tag="IT904_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAP3192635.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICYAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQLQGYQWIDTPHQGQTTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(3586..3870)
                     /gene="ihfB"
                     /locus_tag="IT904_002883"
     CDS             complement(3586..3870)
                     /gene="ihfB"
                     /locus_tag="IT904_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAP3192636.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(4030..5703)
                     /gene="rpsA"
                     /locus_tag="IT904_002884"
     CDS             complement(4030..5703)
                     /gene="rpsA"
                     /locus_tag="IT904_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP3192637.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(5814..6497)
                     /gene="cmk"
                     /locus_tag="IT904_002885"
     CDS             complement(5814..6497)
                     /gene="cmk"
                     /locus_tag="IT904_002885"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP3192638.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(6670..7434)
                     /locus_tag="IT904_002886"
     CDS             complement(6670..7434)
                     /locus_tag="IT904_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP3192639.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRPQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(7603..8886)
                     /gene="aroA"
                     /locus_tag="IT904_002887"
     CDS             complement(7603..8886)
                     /gene="aroA"
                     /locus_tag="IT904_002887"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP3192640.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTGLGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYFVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(8957..10045)
                     /gene="serC"
                     /locus_tag="IT904_002888"
     CDS             complement(8957..10045)
                     /gene="serC"
                     /locus_tag="IT904_002888"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAP3192641.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(10244..10936)
                     /locus_tag="IT904_002889"
     CDS             complement(10244..10936)
                     /locus_tag="IT904_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP3192642.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            11066..12826
                     /locus_tag="IT904_002890"
     CDS             11066..12826
                     /locus_tag="IT904_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAP3192643.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            13232..14089
                     /gene="focA"
                     /locus_tag="IT904_002891"
     CDS             13232..14089
                     /gene="focA"
                     /locus_tag="IT904_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAP3192644.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            14144..>16002
                     /gene="pflB"
                     /locus_tag="IT904_002892"
     CDS             14144..>16002
                     /gene="pflB"
                     /locus_tag="IT904_002892"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP3192645.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNT"
BASE COUNT         4086 a         4118 c         3783 g         4015 t
ORIGIN      
        1 caatgcaggt acgtcacgtc ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc
       61 agtcgcacgc tcgatcacgc gcttaccttc atctttctcc tgctcactgt ccagaatggt
      121 aaacagcgtc tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagcga
      181 tttcagcgga cgcatcagtg caatcattga agagaaaaca acggtaatcg taccggcagt
      241 caggctatcc atgacacttg ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga
      301 ggcgatcagc tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg
      361 aagacgcatt cggttgctga ctttatcaaa gcgtttcgtt tccacttcct gaccaccgaa
      421 aatcaatact tctttgtggc ccttcagcat ttgttctgcg ctggtggtca cctgccccat
      481 ggtgttctgc atgtttttac tgatgttgcg aaaacgcttc gatacaacgc gaatcgcaat
      541 cgaaacaatc ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat
      601 catgatgaac aggccgatga tcgacgcacc ttcacgcaca acagtaatca gtgcgccgga
      661 agaagaagaa gcaacctgtt cggaatcgta ggtaatacgt gacaacagcg tacccgttga
      721 ctgtttgtca aagaatgaaa ctggcattcc catcatgtga ccaaacaggc ggcgacgcat
      781 ggtcattacc acctttcctg atacccagga gatacagtag ctggagacat agctggtgat
      841 accacgtaaa atcatcagcc cgatcaccac cagcggcatc cacaccagca cggagcgatc
      901 tgttttacca aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct
      961 ggctgcgttg aggattaacg ctacgcccgc cacgatcaga cccgctttga aaggcgcaat
     1021 ggttggccac agtcggcgga atgtctgcca cgtagagaga tctttgtcgt tatgcattca
     1081 aaaaaccagc atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa
     1141 ccactgatga taccaacgag gtaaaatttg ctccctgagg ctgctaatcc gccagccttg
     1201 cgctgaaaaa ttgaccgttg tttgaccttg atgtggagta tcaatccatt gatatccttg
     1261 cagttgatag cgatgcttaa ctttgttaga gggcagtcgc catgcgttat agcgcgatgc
     1321 cgatgcgagt gccacttttc cattcactcg ctgaattaat ggcaacgatg atgaggtatt
     1381 actgccatgg tgaggtacct gaagcaatgt tgcctgcact tgctgccagt aacggcttag
     1441 catcttttgt tcagctgggg cttcaatatc accggttaga agaatgctat tcgtcccgtc
     1501 atcaacctta accacacagg aatggttatt tcctttatcg ttgctacctt gtaaaggcca
     1561 gtgcgcgctg aaacgcaatc cttgccattg ccacgcttcg ccacgcacac agggctgatg
     1621 atgttcccag tttaacggac ttctgatcca taacatcggc catatatgca atattgaatc
     1681 cagccctccc cggtgatcca gatgttcatg gctcagaata acgccttccg gttcaagatt
     1741 atgccagtgg agccagggga taatcagttg ttgcccacta tccccttcag gccaggccag
     1801 tcctgtgtca tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc
     1861 gacatcaagc atgtacagct gccactcgtc aggtcgaggt ttttgccaca gcggccagca
     1921 catcagcaag cctccagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca
     1981 taccaccagt aagaaccatg gggaaaatga tagccattgc caacattcag caatgttgat
     2041 ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag
     2101 aaaccataac ccttgctcca ggattaacgg cccgcttaaa tgcacaacca tcgcggcgag
     2161 gatcaacgga accgtgataa atgtcaccaa gggaattgct aatagatttg caataaacga
     2221 ggtcagacta atgccatgaa atatgacgat ttgcacgggc ataagcagaa gtgtgattcc
     2281 cagttgcaga tggatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg
     2341 acagggaaac cactgatacc aaaatatcaa tgccgcgacc gcagcggcag agagccataa
     2401 actttgcgag agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca
     2461 tacatcccag ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc
     2521 caccatggta cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcata
     2581 gcagattccg ccaattaatg gtatttgcca gtggatccag cgcccaggca gaaaaatttg
     2641 tccactgcga atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat
     2701 cgccattaaa tgcgccgttc cagtatcgcg catgatattt ttgatttctt tggggacgga
     2761 taaccgttcc cccataccta aaccaagaat aaccgcattc cacggatagg gttgcagcgt
     2821 tgtttgtagt gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc
     2881 ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa
     2941 gccgccatca ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg
     3001 tccagcacaa accgcttgcg gaagatattc gccatacatc acgagacctg acgcagggaa
     3061 tatacgtcga ccttgtagat gagtaatttg accgtaatga gtggtcatgc cgtcagttgc
     3121 agtgatctca acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat
     3181 ttgctttgct gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac
     3241 ggttttaaca gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa
     3301 tgtcccaggc aattggggca aaatcagcaa cggaaaaatt ccgctaatta tgcatacacc
     3361 gactgtcgtt attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc
     3421 ggttgtctgg tgggaatttt gcgttccccg aggcatattc agaagaattt aacgaaaggg
     3481 tgaaaactgt tcaaaactct gcgaggcagc ttccagttcc agattagaga aatgaaaaaa
     3541 gcacccgaca ggtgcttttc tctcgttcaa gtttgagtaa aaaacttaac cgtaaatatt
     3601 ggcgcgatcg cgcagttctt taccaggttt aaagtgagga acgtattttc cttccagttc
     3661 tactttatcg ccagtcttcg gattacgtcc ggtacgtggt gcgcggtagt gcaaagagaa
     3721 actgccgaaa ccgcggattt caatacgctc gccctgcgca agagtcgagg ccatatgctc
     3781 cagcatctct tttactgcat cttcaaccgt cttggcggga atgtgcgatt gctgggtggc
     3841 aagtctttct atcaattctg acttggtcat gattcctccg gttccttaaa ggcaaattaa
     3901 gcggctgctg ttgctgcatt gattaagggc ggccgtagcc gcccttagtg cttgattaca
     3961 ggacgaaacc tgcaatctgt caagtaaact caacaaactt cggaataaaa atcccgaaga
     4021 gtcagagaat tactcgcctt tagctgcttt gaaagcttca gccattgcgt tgttggagaa
     4081 gtttgcatct tcctgtttgt taacagttgc gattgcatct ttctcgtcag cttcgtcttt
     4141 cgcacgaaca gacaggctga ttgcgcggtt tttacgatca acgccggtga atttagcttc
     4201 aacttcgtcg ccaacgctca gaaccagggt agcgtcttca acgcggtcac gggatgcttc
     4261 agaagcacgc aggtaacctt caacgccgtc agccagttct acggttgcgc ctttagcgtc
     4321 aactgcagtt actttaccgg ttacgatagc gcctttcttg ttcagagcaa cccagttgtt
     4381 gaacggatct tctgcgagct gtttaacgcc cagggagata cgttcacgtt ctgcgtcaac
     4441 ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat tcacgaactg cttcttcgcc
     4501 tgcaacgttc caggagatgt cagacaggtg aaccaggccg tcgatgccgc cgtccaggcc
     4561 gatgaagata ccgaagtcag tgatagactt gattttacct tcaacacggt cgcccttgtt
     4621 gtgggtttcc gcgaactgct gccacgggtt agctttgcac tgtttcagac ccagggagat
     4681 acgacgacgt tcttcgtcga tatccagaac cataacttcc actacatcgc caacgttaac
     4741 aactttggac gggtggatgt ttttgttggt ccaatccatt tcggaaacgt gtaccaggcc
     4801 ttcaacgcct tcttcgattt caacgaagca gccgtagtcg gtcaggttgg tcacgcgacc
     4861 agtcagtttg gtaccttccg gataacgttt agcgatagct acccacggat cttcgcccag
     4921 ctgtttcagg cccagggata cacgggtacg ttcgcggtcg aacttcagca ctttaacagt
     4981 gatttcgtcg cccacgttga cgatttcgct cggatgctta acgcgtttcc aggccatgtc
     5041 agtgatgtgc agcaggccgt caacgccgcc cagatcaacg aatgcaccgt agtcagtgag
     5101 gttcttaacg atacctttaa cttccatgcc ttcctgcagg ttttccagca gctgatcgcg
     5161 ctctgcgctg ttttcggatt cgataacggc acgacgagaa acaacaacgt tgttgcgctt
     5221 ctgatccagc ttgattactt taaattcaag ctctttgcct tccaggtgca gagtgtcacg
     5281 caccggacga acgtctacca gagaacctgg caggaacgca cgaataccgt tcagctcaac
     5341 agtgaagccg cccttaactt tgccgttgat aacaccggta acagtttcag catcttcgta
     5401 agctttttcc agcgtgatcc aggcttcgtg acgtttagct ttctcacggg acagcagagt
     5461 ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca acttcgtcac ctacctggat
     5521 ttccagctcg ccctgggcgt ttttgaactg ctcagccggg atggcggact cagatttcag
     5581 accagcgtca accagtacta cgtctttgtc gatagcaaca acaacgccac gaacgataga
     5641 acccgggcgg gtttcgattt cttttaagga ctcttcaaag agttgagcaa aagattcagt
     5701 catgtttaat cttcaggttt atatttaacg tccacctggc tccatgccgg atggggttgt
     5761 ttaacatacc cgctgtcatt ccattgcaac gggggtactg caaattcggt cgcttatgcg
     5821 agagccaatt tctggcgcgc gtattgtagc gctttttcaa tcacttgctc aatgcttaag
     5881 gtggtggaat ccaacactaa agcatcggct gccggaacca gtggcgctac cgctcggtta
     5941 cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc gctcaaagtt aacactaaag
     6001 cccttctcct gcaactgtag catgcggcga tgcgcacgtt cttccgagga ggcgtcaagg
     6061 aaaattttca ctggtgcatc agggaatacc accgttccca tgtcgcggcc atcggcaatc
     6121 agacctggta attcgcgaaa cgcgcgttgg cgacgcaata atgcttcacg aacgcgtggg
     6181 aatgccgcga cttgtgaagc tgcattcgcc acttcctgag tacgaatttc gccgctgaca
     6241 tcttcccctt cgaggatcac ttccagattg ccattggtcg acacaaaacg tacatccaga
     6301 tgggatgcca gcggtaccag cgcatcttcc gacgcaacat caacatggtg atgtaatgcc
     6361 gccaatgcca gtacgcgata aattgcaccc gagtccagca gatgccattg caacgcttcc
     6421 gccatagcct tacacaaggt gcctttccct gcaccgcttg ggccatcaat ggtaataacc
     6481 ggggcaattg ccgtcatctt tatctcctta aaacaggcgt accgttaaca taaccgcgcg
     6541 cattatacgc gccgacgcca caaatcgtta acgttgtgtg caaaaaccag ttttgtctgt
     6601 attcagatgg ggatttattg cgcaattata gcgggctggc agaagaccag cccgaaagat
     6661 gacaatgatt tacttaccag aggcgatacg atcacggatg tgttgcgcac gctctgttga
     6721 tggtgggtga gagtcaaaca tggactgggg gcgaccgcca tccaggctag ccagtttttc
     6781 aaagctgcca accagcccct gggtgctgat gccacgtttc ttcaacagat caaaggagaa
     6841 atcatctgct tcggactctt tatcacggga gtatttagca ttgatagcgc cttctgcgat
     6901 atcacctaat tgtgagcggg aaagctggga agccacaccg ctggtagctg aaatggcatc
     6961 gcgtgcggca acgatcgcat aagaagcttt catttcagcc agcgagtgac ccaacgcgac
     7021 gtggcccagt tcatggccca gaacgccttc aatttcgttg tcattcatca tgtccatcag
     7081 gccactgtag acacgaacac agccgttcgc catcgcccat gcgttgacgt cgctggtcat
     7141 ataaacctta tagttgaccg gcgtgccatt aatattgtta cccaatgctt tggcgatttt
     7201 tgccagacgt ttaccgtact tgctggattt acttgcgact tgattgccgc tgtccatttc
     7261 ggcacagccc tgattggcaa tcgcttttgc atcggcatcg cttaatgttg ctgctttata
     7321 ggcatttaaa ccggagctga tagccatatt ggtatcaata ccgtgggtat tttgacaccc
     7381 tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat ttagtattct tcatgtgaat
     7441 catccgttga ttaatcatat ataaatatta tgtatcaaca acttacattg acaagcgacg
     7501 ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa cgccgacaat gtgccgacgt
     7561 tttgataaag aaagatttgg ctatttattg cccgttgttc attcaggctg cctggctaat
     7621 ccgcgccagc tgctcgaaat aatccggaaa tgttttggcc gtgcatttgg gatcaagaat
     7681 cgtcactggt gtatctgaca acgccaccag cgagaaacac atcgccatcc ggtgatcatt
     7741 gtatgtcgcg atctcggcaa agttcagttt ttccggtgga gtgatacgaa tgtaatcgtg
     7801 cccctcttct acttccgcac cgactttacg cagttctgtt gccatcgcaa acaggcgatc
     7861 ggtttcttta acacgccagt tatagatatt gcgcagcgtg gtggtgcctt ttgcaaataa
     7921 cgccgccgtg gcaatggtca tcgccgcatc gggaatatgg ttcatatcca tatcaatagc
     7981 gttcagttca ccacgcgtgc aggaaatata atcatcgccc cagcaaatgg tcgcgcccat
     8041 tttttccagc acatcagcaa agcgaatatc accctgcata ctgttacgtc caataccggt
     8101 cacttttaca gtgccgcctt tgattgctgc tgctgccaga aagtaagaag ccgaagatgc
     8161 atcgccttcg acaaaataag tacccggaga ctgataagac tgcccgcctt ttacgacaaa
     8221 ttgttgatag tgctgatttt caatttcaac accaaacgtc ttcatcagat tgagtgtgat
     8281 gtcgatataa ggtttagaaa ccagatcgcc tttaatacga atcaccgtat cttccggcgc
     8341 aagaggcgca gtcattaaca gtgcggtgag gaattggctg gaaacggagc catcaacgtc
     8401 aacgttgccg ccggtaaagc cgccctgtaa acgcaacggc ggataatttt cttgttccag
     8461 gtaagtgatc ttcgccccgc ccaggcgcag agcatccacc agatgaccaa tcgggcgttc
     8521 tttcatacgc ggctcaccgg tcagcacaat atcattgcta cccagacaaa gagctgccgc
     8581 cagcggacgc attgccgttc cggcgttacc gaggaacaac tccagggcac cttctgcgtg
     8641 taatggaccg ccgttaccga taatttcgca acgcgtacga tcggctgaaa gcgtatagct
     8701 tacccctaac cctgttaatg cattcagcat atggcgcacg tcatcgctat ccagcagatt
     8761 ggttaatact gttttgccgt gtgctaatgc cgccagcaat aaagcgcggt tagaaacgct
     8821 cttggaaccg ggcagattaa tagtgccatc gacacgagcg atgggttgta acgtcaggga
     8881 ttccatgaac tcaactctct acaacagaaa taaaaacccc acaggctggc tgtggggatt
     8941 aagcaaaatt tcggcattag ccgtgacggc gttcgaactc aaccatgaag tctgtcagcg
     9001 ctttaacgcc ttccagcggc atggcgttat aaatagacgc gcgcattccg ccgaccacac
     9061 ggtgaccttt cagtgcatga aggccagcag caaaagactc ttcaaggaac aatttgtcaa
     9121 gcgcactgtc cgccaactgg aacggcacgt tcatccgcga acggttagct ttcgccacgt
     9181 cattgcggta gaaatcgctg ttatcaatca ccccatatag cagttctgct ttttgctgat
     9241 tgattttatc catttcagct acaccgccgt tcgctttcag ccatttaaag accagaccag
     9301 atagatacca ggcaaatgtc ggcggcgtgt taaacatgga gccgttatcg ttgaggatgg
     9361 aataatccag aatcgacgga cacgcgatat tcgctttgcc cagcaaatct tcacgaacga
     9421 tgacgattgt caggccagcc gggccgatat ttttctgcgc gccagcgtaa attacaccat
     9481 aacggctgac gtcaatcgga cgggaaagaa tggttgaaga gaagtcggcg gcgaccacca
     9541 catctttgcc gaagtctggc gtctcgtcga tggcgatacc gtcgatggtt tcattcgggc
     9601 agtaatgcat ataagccgca ttatcagaga gttgccattc acgcattggc ttaaccgcgc
     9661 gcagaccatc aacagtcact ttggcgtcaa agacattagg cgtgcagtat tttttcgctt
     9721 ctttaatggc acttgccgcc cagtaaccgg catcaacata atctgcggtg gttttatcac
     9781 cgagaatatt cagcggtacc gcagcaaact gaccgcgacc accgccatgg cagaataata
     9841 ccttgtagtt ggaggggaca ttaagaagat cgcgaaaatc cttctcggct tcctctgcaa
     9901 cctgaatgaa ctctttgcca cggtgactca cttccatcac cgacgtacca agaccgttcc
     9961 agtcgcgcag ttcctgttga gcctgtttaa gcacctctgc cggtagcatt gccggaccag
    10021 aactaaaatt gaagatttga gccatttccc ctcaccacgt tgcgttgcta ttaacccgcg
    10081 gtcaatcgat tgcgaccaca ggtaatacat tattcctgtg gctatcggtt ttatcattca
    10141 gtgacacaca tcgcaatgag taaaaccgtt gagggcaaaa atgtggcttg catcacacaa
    10201 aagaatctct cggattatta gcaaaaagcc gatgttttta ctgtcaggtc acccgttttg
    10261 cccgacttgc cttcgtccat tctggatttg cacagcgcgg acataattgt ttttcccccg
    10321 ctttcatatg aatgatttca ctacaacgaa tacaggcata gtccgccgac tccggcacca
    10381 ctttgtaacg ctgcgtacaa tcactgggta aaataagtaa acccggtttc accttgtcat
    10441 cttcaaagaa atagacactg atttgcccgt tggtttcgag aatcgccaga cgtacctgcc
    10501 ccagctgctc cacgccacgc aatcgtagct ccataaagaa ctcaaattcc gtcatgttgg
    10561 agttattgag tttcgaccag gccagctcgc catcttcaat aatgacaact ggcttgcctt
    10621 ccagaagatc ttccagtttt tcactgtgcg ccatcaacca cattaccagg cggtataaca
    10681 acgccagagt aataaagacg ataagtaccg ggaccatcgg cacatcatca taaaacgcca
    10741 catctcccgc cgctgatccc agcgtcagaa tgattaaaac ttcaaacagc gacatctgcc
    10801 gcacaccgcg tcttccggtc attttgagga acaaaaagac cagtacaaag gtataaagac
    10861 tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc atacggtgga
    10921 gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag catagtcaac
    10981 agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat tattgcgcgc
    11041 tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca aagatgccgc
    11101 tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct ttcagattga
    11161 agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc gcgacaaaga
    11221 gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc tggcttctgc
    11281 actcggtgaa tatttcgagc gtctctcaac caactacttt tttgcggact tctggctggg
    11341 cgaaaccatc gccaacggtc cgttcgtgca ttatcccaac gaaaaatggt tcccactgac
    11401 cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcgt tttatgatcc
    11461 ggagaatgaa ctgaccggca gcatgctgat tgacctacaa tccggtaacg aagatcgtgg
    11521 tatttgcggc ctgccgttta cgcgtcagtc cgacaatcag accgtttata ttccgatgaa
    11581 tatcattggt aacctgtacg tctccaacgg tatgtccgca ggtaataccc gcaacgaagc
    11641 acgcgttcag ggattgtctg aagttttcga acgctacgtg aaaaaccgca ttattgctga
    11701 aagcatcagc ctgccagaga tcccggcaga cgtgctggcg cgttacccag cagtagttga
    11761 agccatcgaa acgctggaag cggagggttt cccaatcttc gcttatgacg gttcgcttgg
    11821 cggccagtat ccggtgattt gcgtggtact gttcaatcct gctaacggta cctgctttgc
    11881 ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga ccgagctgct
    11941 gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg atgatgaaga
    12001 agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt taatctcctg
    12061 ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct ccggcaccac
    12121 ggaagaagag ttcgccacac tgatggctat cttcaacaaa gaagataaag aagtttatat
    12181 tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg gcatgtccga
    12241 tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc atttacgtga
    12301 aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga acctcatcga
    12361 gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt tgggtctggc
    12421 gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag ccatgctggc
    12481 gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga tggagtttaa
    12541 ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa cgttgttatt
    12601 actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg ttcgcatgta
    12661 cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg cgttttacgg
    12721 cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt tgttgaaggc
    12781 ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc aaatagccta
    12841 cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt taatgccccg
    12901 ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata tcggtaattt
    12961 gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc ttttaattaa
    13021 ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta aaatttattt
    13081 ttatttggat aatcaaatat ttactccgta tttgcataaa aaccatgcga gttacgggcc
    13141 tataagccag gcgagatatg atctatatca atttctcatc tataatgctt tgttagtatc
    13201 tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt ttgatctttt
    13261 acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag caacgaaaca
    13321 tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa tcgcattcgt
    13381 cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg caaaactggt
    13441 tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag ccgatctctt
    13501 tacttccacc gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct ggggtcagtt
    13561 ggcgaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc tgtttgtact
    13621 tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac taaacgtcct
    13681 acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc ttggtatcct
    13741 ggcaaacctg atggtatgtc tagcagtatg gatgagttat tctggccgca gcctgatgga
    13801 caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt ttgagcacag
    13861 tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg catccccgga
    13921 attttggacc gcagtcggtt ctgcaccgga aaatttttct cacctgaccg tgatgaattt
    13981 catcactgat aacctgattc cggttacgat cggcaacatt atcggtggtg gtttgttggt
    14041 tgggttgaca tactgggtca tttacctgcg tgaaaacgac caccattaat ggttgtcgaa
    14101 gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg agcttaatga
    14161 aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg aagtaaacgt
    14221 ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct tcctggctgg
    14281 cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta aactggaaaa
    14341 ccgcactcac gcgccagttg actttgacac cgctgttgct tccaccatca cctctcacga
    14401 cgctggctac atcaacaagc agcttgagaa aatcgttggt ctgcagactg aagctccgct
    14461 gaaacgtgct cttatcccgt ttggtggtat caaaatgatc gaaggttcct gcaaagcgta
    14521 caaccgcgaa ctggatccga tgatcaaaaa aatcttcact gaataccgta aaactcacaa
    14581 ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat ctggtgttct
    14641 gaccggtctg ccagatgcat atggccgtgg ccgtatcatc ggtgactacc gtcgcgttgc
    14701 gctgtacggt atcgactacc tgatgaaaga caaactggca cagttcactt ctctgcaggc
    14761 tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag aaatcgctga
    14821 acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct acgacatctc
    14881 tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct acctggctgc
    14941 tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct tcctggatgt
    15001 gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc aggaaatggt
    15061 tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg aatacgatga
    15121 actgttctct ggcgacccga tctgggcaac cgaatctatc ggtggtatgg gcctcgacgg
    15181 tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca ccatgggtcc
    15241 gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga acttcaagaa
    15301 attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg atgacctgat
    15361 gcgtccggac ttcaacaacg atgactacgc tattgcttgc tgcgtaagcc cgatgatcgt
    15421 tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca tgctgtacgc
    15481 aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt ctgaaccgat
    15541 caaaggcgat gtcctgaact atgatgaagt gatggagcgc atggatcact tcatggactg
    15601 gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg acaagtacag
    15661 ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca tggcgtgtgg
    15721 tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg cgaaagttaa
    15781 accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg aatacccgca
    15841 gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag aacgtttcat
    15901 gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt ctgttctgac
    15961 catcacttct aacgttgtgt atggtaagaa aacgggtaac ac
//
LOCUS       DACAPB010000103        15814 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.103, whole genome shotgun sequence.
ACCESSION   DACAPB010000103 DACAPB010000000
VERSION     DACAPB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.103"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..746)
                     /gene="tal"
                     /locus_tag="IT904_002893"
     CDS             complement(<1..746)
                     /gene="tal"
                     /locus_tag="IT904_002893"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP3192646.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALL"
     gene            1025..2455
                     /locus_tag="IT904_002894"
     CDS             1025..2455
                     /locus_tag="IT904_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAP3192647.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2525..3301
                     /gene="yaaA"
                     /locus_tag="IT904_002895"
     CDS             2525..3301
                     /gene="yaaA"
                     /locus_tag="IT904_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAP3192648.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLASINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDNFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(3454..3750)
                     /locus_tag="IT904_002896"
     CDS             complement(3454..3750)
                     /locus_tag="IT904_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP3192649.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(3964..5250)
                     /gene="thrC"
                     /locus_tag="IT904_002897"
     CDS             complement(3964..5250)
                     /gene="thrC"
                     /locus_tag="IT904_002897"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAP3192650.1"
                     /translation="MKLYNLKDHSEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(5251..6183)
                     /gene="thrB"
                     /locus_tag="IT904_002898"
     CDS             complement(5251..6183)
                     /gene="thrB"
                     /locus_tag="IT904_002898"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAP3192651.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(6185..8647)
                     /gene="thrA"
                     /locus_tag="IT904_002899"
     CDS             complement(6185..8647)
                     /gene="thrA"
                     /locus_tag="IT904_002899"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAP3192652.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLTAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLESLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(8728..8793)
                     /gene="thrL"
                     /locus_tag="IT904_002900"
     CDS             complement(8728..8793)
                     /gene="thrL"
                     /locus_tag="IT904_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAP3192653.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9007..9693)
                     /locus_tag="IT904_002901"
     CDS             complement(9007..9693)
                     /locus_tag="IT904_002901"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP3192654.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSPAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(10093..10233)
                     /locus_tag="IT904_002902"
     CDS             complement(10093..10233)
                     /locus_tag="IT904_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192655.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTADILTILT
                     AT"
     gene            10329..11045
                     /gene="arcA"
                     /locus_tag="IT904_002903"
     CDS             10329..11045
                     /gene="arcA"
                     /locus_tag="IT904_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAP3192656.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(11104..12456)
                     /gene="creD"
                     /locus_tag="IT904_002904"
     CDS             complement(11104..12456)
                     /gene="creD"
                     /locus_tag="IT904_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAP3192657.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLSESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(12514..13938)
                     /gene="creC"
                     /locus_tag="IT904_002905"
     CDS             complement(12514..13938)
                     /gene="creC"
                     /locus_tag="IT904_002905"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAP3192658.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(13938..14627)
                     /gene="creB"
                     /locus_tag="IT904_002906"
     CDS             complement(13938..14627)
                     /gene="creB"
                     /locus_tag="IT904_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAP3192659.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(14640..15113)
                     /gene="creA"
                     /locus_tag="IT904_002907"
     CDS             complement(14640..15113)
                     /gene="creA"
                     /locus_tag="IT904_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAP3192660.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            15324..>15814
                     /locus_tag="IT904_002908"
     CDS             15324..>15814
                     /locus_tag="IT904_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3192661.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQF"
BASE COUNT         3832 a         4244 c         3835 g         3903 t
ORIGIN      
        1 agcagtgccg gtgcgatggt cagacggtcg cagcctgcca gttccagaat ttcgccgatg
       61 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
      121 atttcagata cagaaaccac acccggatct tctgccggag cgtactcttt cttatcggta
      181 ttcgctttgt accagtcaag aatacggcca acaaacggcg agatcaggaa cacgcccgct
      241 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatacct
      301 tctttttcca gctgttctgc agcacgaata ccctgccagg tagaagccag tttgatcaga
      361 atacgatcgt tgctaatacc tgcatcgttg tagagtttga tcaggcgttt tgctttcgca
      421 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
      481 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
      541 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
      601 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
      661 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
      721 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
      781 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
      841 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
      901 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
      961 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     1021 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     1081 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     1141 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     1201 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     1261 cgttgcgctg gcaattaccg ccggtggacc tggagccgtc ttctggatgt gggttgccgc
     1321 gtttatcggc atggcgacct cgtttgccga atgttccctc gcacagcttt ataaagaacg
     1381 tgatgccaat gggcagtttc gtggcggacc ggcatggtat atggcgcgcg ggctggggat
     1441 gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     1501 cggaattcag gcgaacgccg ttgcgcgagc cctgagtttt tcttttgatt ttcccccgct
     1561 ggtgacaggt attattctcg ctgtctttgc tctgctggcg atcactcgcg gtcttcatgg
     1621 cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg ataatctggg tactgaccag
     1681 cctggtgatt tgcgtaatga atatcgggca acttccccac gtcatttggt ctatttttga
     1741 gagtgctttt ggctggcagg aagcggcagg cggcgcggcg ggatatacct taagccaggc
     1801 gattactaac ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg ggtcgacgcc
     1861 aaacgcggca gcggcagcgg cgtcctggcc tccgcatccg gcagcgcaag ggattgtcca
     1921 gatgattggc atttttatcg acaccctggt catctgtacg gcaagcgcca tgctgatatt
     1981 actggcgggt aacggcacaa cctacatgcc gctggaaggt attcagctta tccagaaggc
     2041 gatgcgggtg ctaatgggtt cctggggtgc tgagtttgtt accctcgtgg ttattctgtt
     2101 tgccttcagc tccatcgttg ccaactacat ttacgccgaa aacaatctct tctttttacg
     2161 cctgaacaac cctaaagcga tctggtgttt gcggatctgc accttcgcaa cggtcatcgg
     2221 cggcaccttg ctaagtcttc cgctgatgtg gcaactggca gatatcataa tggcctgcat
     2281 ggctattacc aatttgaccg ccattttact gctctcgcct gtggttcata ccattgccag
     2341 tgattatcta cgccagcgta aactcggcgt gcgcccggtg tttgatccgt tgcgttatcc
     2401 ggatatcggc cgccagcttt ctccggacgc gtgggatgat gtttcgcagg agtaatcaca
     2461 actatcgatc aactcattct ctttttttgc taaagtcggc ataaatttcc tgcaaggact
     2521 ggatatgctg attcttattt cacctgcgaa aacgcttgat taccaaagcc cgttgaccac
     2581 cacgcgctat acgctgccgg agctgttaga caattcccag cagttgatcc atgaagcgcg
     2641 gaaactgacg cctccgcaga ttagcacgct gatgcgcatc agcgacaaac tggcgagtat
     2701 caacgccgct cgctttcatg actggcagcc agatttcacg ccggagaatg cccgccaggc
     2761 gattctggcg tttaaaggtg atgtctacac cggcttgcag gccgaaactt tcagcgaaga
     2821 caatttcgat tttgcccaac agcatttgcg aatgctttcc ggcttgtatg gcgtactgcg
     2881 cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg atccgtcttg agaatgcccg
     2941 agggaaagat ctgtatcaat tctggggaga tatcatcacc aacaagctga acgaggcgct
     3001 cgcagcacaa ggcgataatg tggtgattaa tctggcgtca gatgagtatt ttaaatcggt
     3061 gaagccgaag aaattgaatg ccgagattat caagccggtg ttcctcgatg agaagaacgg
     3121 caaatttaag atcatcagct tctacgctaa gaaagcacgc ggtctgatga gtcgttttat
     3181 tattgaaaat cggctgacca aaccagagca actgactggt tttaatagcg aaggttactt
     3241 ctttgatgaa gcttcctcca gcaatggaga actggtgttt aaacgctacg agcagcgtta
     3301 agtttgcagt caatgccgga tgcggcgtga acgccttatc cggcctacaa aagcatgcag
     3361 attcaatata ttgcagaatt cacgtaggcc tgataagacg cgccagcgtc gcatcaggcg
     3421 ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg
     3481 accgccgtga tgatcatgag gagctttctt atggtgacgc ggcggtggcg gcggtccgtg
     3541 tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca
     3601 gtgaccgcca tcccagtaat agccacgatt atcacgatcg cctatctgta attttactga
     3661 cgggactaac gtaatttccg cagcctgcgc tgccatggga gcgaccagaa ccagggaaag
     3721 tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg
     3781 ggtcgatatc cggagattac gcgatgagaa cggcggttgt tattctccgc aatcctaata
     3841 tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgtttct ctccatattt
     3901 tcttcacaaa aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaaaag
     3961 atattactga tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg
     4021 tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc
     4081 gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc
     4141 atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc
     4201 cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac
     4261 ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact cttcaacacg
     4321 cggccagttg tttggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt
     4381 gggtgaccac tgaccatcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc
     4441 aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc
     4501 gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgcg gcagctgcgc
     4561 aacagcttca aagtagtagc aaatctgcgc cagcaaccgg ctgatgttaa tcgagttagc
     4621 tgagtttaac cccagcgcca ctttcagctc ttcatcatca aacgcctgct tcaccagcgc
     4681 ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaatgtaca
     4741 gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac
     4801 attcggtaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt
     4861 cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca taaagcgacc
     4921 gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct
     4981 ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat
     5041 ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa
     5101 atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt catgcggaaa
     5161 aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc
     5221 gctgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt
     5281 atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca
     5341 gtcggcaacg cgctgggcgg tatccggctt gtcacacaga gcgaacaagg tcgggccgga
     5401 gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa
     5461 gccaggcagt aaccgttcac ggtagggttc tgcgataaca tctttcatca gcttcgcggc
     5521 aagctcaggc tgacgggaat agcaggcgtg aatgaagcct gccagatgtc gcccgtgcgc
     5581 aatgcaatcc tggcggcgat actgcgccgg taaaatagcc cgggcttctg ccgtcgagac
     5641 tttaatcccc ggatacgcca gcacccacag ccactcatca aaccctggca cttgctggct
     5701 gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg gtgccacgtt
     5761 gtcgtaatga atgctgccgg agattcgtcc ttccagctcg cccatcaaag ccagcaaacg
     5821 agtgtcatta agcggcttgc cacagtgttc attcatcgcc atcagcgccg cgaccaccga
     5881 acaggcgctg gagcctaagc ccgagccgat cggcatattc ttttccagag tcatcgccac
     5941 tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc
     6001 ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc
     6061 tgccgcctca accgtgacta catctccgag caatgcacca tcaacaggtg tcaccgccgc
     6121 cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac
     6181 catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag acaccggcag
     6241 ctgtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt
     6301 ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat
     6361 ccacttcggc aatcttcacg cggcaggcac catcttcatc aatattgcca acatagcgca
     6421 aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg
     6481 acagattcgc cataaaagcg gcaacatcac cctcagcgtt aaactctgcg ggcagcacag
     6541 gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg agaatcaata
     6601 gcttacgcgc tacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca
     6661 tttcccgcgc cagagtagtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga
     6721 tataagaaag cgaacctgaa agaatgccgg agaacttcat caattcatca ccagcattga
     6781 gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg tcatagagga
     6841 atttacgccg cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg
     6901 tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaagaag tcggcatatt
     6961 gatccgccac tgcctggctg gaagtgcagt caacaatgac cgggttcagc agatgatatt
     7021 ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt
     7081 cctgccagtt ttccaggttt aggccatgca cattggtaag cagagccttc gagttggcaa
     7141 cgccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt
     7201 gctccagcag cgcaccgcca acgccaccga cgccaatcac aaatacttcg ataacctgat
     7261 cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta
     7321 ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg ttgatattgg
     7381 cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc ataccatcac
     7441 ctaccaccga gataatggcc agccgttccg tcaccgccag cgactccagt aagccttctt
     7501 tcagttccag gtagaactct tcctgcattg cccgttcagc tcgcacacag tcgctttgcg
     7561 gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac
     7621 gggcgcgtga catcgctgca aacacgcgcg ccgccatgcc gaccattcct ttcatccccg
     7681 ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt
     7741 cgtcttcatc acggctggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt
     7801 taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt
     7861 tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg
     7921 catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc
     7981 aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc
     8041 caagcaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc agcaccatgt
     8101 gatcagccgg aatacgactt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag
     8161 attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg gtaacgttgt
     8221 gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa
     8281 tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca tgcaggacat
     8341 gttttatttg ggcaaattcc tggtcgacga aagttttcag ttgcgccagc gggaatcccg
     8401 gctgggcggc ggtgagtccc gtcagaagtt cggcaaaaat acgttcggca tcgctgatat
     8461 tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga
     8521 ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa
     8581 tatcggcaac ccgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca
     8641 ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact gtcaggtgcg
     8701 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
     8761 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
     8821 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
     8881 taatttactc acggcaggta accagttcag aagctgctat cagacactct ttttttaatc
     8941 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
     9001 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
     9061 gtctcgttgc tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc
     9121 tgccactgcc agagtcgtca gcaatgccat ggctcgttcg cgtaaagctt gcagttgatg
     9181 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
     9241 atagaccatc accgcctgcc ccagattgag cgaaggataa tctgccacca tcggcacacc
     9301 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
     9361 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggta ccagttctac
     9421 tggcgtagcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
     9481 gacatcgtgt aacgattcag ccagtgtcgg gaaaacttta atattatcaa taatatcacc
     9541 agatccatgt gcaacccagc gggtggctgg ctccaggtgt gccggactat cgacaatccg
     9601 cagttcgcta aaccccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
     9661 ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga ccaaatgacc
     9721 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat ccttaacgta
     9781 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc taactgtact
     9841 aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
     9901 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
     9961 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
    10021 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
    10081 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg tcagccgttt ttgacactgt
    10141 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
    10201 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
    10261 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
    10321 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
    10381 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
    10441 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
    10501 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
    10561 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
    10621 atgactacat caccaaaccg ttcaacccgc gtgaactgac tattcgtgca cgcaacctgc
    10681 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
    10741 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
    10801 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
    10861 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccgcacg
    10921 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
    10981 cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt gatctggaag
    11041 attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt ttatttttca
    11101 accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt
    11161 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
    11221 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
    11281 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
    11341 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
    11401 tgccacggta aaaccggtat gttcagaaag cgccagcaaa agcaaataaa acatcaccaa
    11461 tgaaagcccc accagcaaat attgcattgg gtgtaagcgt tgcgcggtga gcgtttcaaa
    11521 aacaaagaac gccataaaag tcagcgcaat cagcagaatg gcatacttag tcgcccggtc
    11581 agttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
    11641 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttatttgcaa accagctgct
    11701 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggca aaaaatcacc
    11761 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
    11821 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
    11881 cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
    11941 ggtgccgggt tcaatggtca gcgccgttcc gttaacttca ggcgctttca ccacaccaat
    12001 accacgcgca tccccgacgc taatcacaat aaatggcttg ccaaaggtga tatttggcgc
    12061 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
    12121 gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc catcaaccat
    12181 caatgactca gataaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc
    12241 ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa cgagtttttg
    12301 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
    12361 gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacaa
    12421 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
    12481 aggttacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
    12541 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
    12601 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
    12661 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
    12721 atccagcacc ttaagggcga cgtgttcctg atccacttcg gcgcttagcg ttatgcgacc
    12781 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gctccagtaa
    12841 cgccggttca gcagcaacat taacctcagt gggcataaca tgtagagtga tgtttttttc
    12901 tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca catcaacaac
    12961 agtcaaaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
    13021 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcggta aaacgagcca ccacttccgg
    13081 tggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
    13141 ctcatgagtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
    13201 catactttcc agcgcctgcg caagcttacg caactcgcta ctgccgagtt caggaagagg
    13261 aacgggctta ttgtcagtga cggaatcggc atagcgagtg agccgggcaa tggagcggtt
    13321 gatccaccaa accatacctg cgccaatcac cagtgcaatc cccagcaaaa tggcgctggc
    13381 ccataaaatt cgccgctcgc tacgcttaat gaccggagcc atcgccgcgt tcggtttgcc
    13441 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    13501 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
    13561 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    13621 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
    13681 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
    13741 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    13801 tgcggtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    13861 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    13921 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    13981 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
    14041 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat tagttgctgg
    14101 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    14161 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    14221 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    14281 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    14341 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    14401 aacgccggat ggagcgccag taactggcgg cataattcaa agccgctgat atcaggcaaa
    14461 ccaacatcga gaatcatgac gtcgggagcc tgctggcgag ctttatccag caccggcaag
    14521 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
    14581 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
    14641 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    14701 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    14761 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    14821 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatagcc
    14881 gcatcggagg tatcttccgc cagacccaat ccccctttaa taccacctgt tttcgcccgg
    14941 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    15001 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    15061 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    15121 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    15181 acaaaatctc aatactttta tttccgtcaa gacctaaaac atactctact aagaaaaaaa
    15241 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
    15301 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    15361 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    15421 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    15481 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    15541 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    15601 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    15661 gtattcgccc gccgctgcgt ctgggtgaat tcactatgcc agagcataaa tttgtcaccc
    15721 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    15781 ctgatttccg ccatgaaatg cgttatcagt tctg
//
LOCUS       DACAPB010000104        15621 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.104, whole genome shotgun sequence.
ACCESSION   DACAPB010000104 DACAPB010000000
VERSION     DACAPB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.104"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..428)
                     /gene="slyA"
                     /locus_tag="IT904_002909"
     CDS             complement(<1..428)
                     /gene="slyA"
                     /locus_tag="IT904_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAP3192662.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQA"
     gene            629..865
                     /locus_tag="IT904_002910"
     CDS             629..865
                     /locus_tag="IT904_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAP3192663.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            868..1725
                     /locus_tag="IT904_002911"
     CDS             868..1725
                     /locus_tag="IT904_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP3192664.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1725..3737
                     /locus_tag="IT904_002912"
     CDS             1725..3737
                     /locus_tag="IT904_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP3192665.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFIGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3738..4259)
                     /gene="sodC"
                     /locus_tag="IT904_002913"
     CDS             complement(3738..4259)
                     /gene="sodC"
                     /locus_tag="IT904_002913"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAP3192666.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4340..5236)
                     /locus_tag="IT904_002914"
     CDS             complement(4340..5236)
                     /locus_tag="IT904_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP3192667.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5285..5524)
                     /locus_tag="IT904_002915"
     CDS             complement(5285..5524)
                     /locus_tag="IT904_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAP3192668.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5627..6226
                     /locus_tag="IT904_002916"
     CDS             5627..6226
                     /locus_tag="IT904_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3192669.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6263..7360
                     /gene="nemA"
                     /locus_tag="IT904_002917"
     CDS             6263..7360
                     /gene="nemA"
                     /locus_tag="IT904_002917"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAP3192670.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDISTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            7441..7848
                     /gene="gloA"
                     /locus_tag="IT904_002918"
     CDS             7441..7848
                     /gene="gloA"
                     /locus_tag="IT904_002918"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP3192671.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7951..8598
                     /gene="rnt"
                     /locus_tag="IT904_002919"
     CDS             7951..8598
                     /gene="rnt"
                     /locus_tag="IT904_002919"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAP3192672.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8691..13307
                     /locus_tag="IT904_002920"
     CDS             8691..13307
                     /locus_tag="IT904_002920"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP3192673.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQPQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(13358..13705)
                     /gene="grxD"
                     /locus_tag="IT904_002921"
     CDS             complement(13358..13705)
                     /gene="grxD"
                     /locus_tag="IT904_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAP3192674.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14039..14854
                     /locus_tag="IT904_002922"
     CDS             14039..14854
                     /locus_tag="IT904_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP3192675.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14982..15563
                     /gene="sodB"
                     /locus_tag="IT904_002923"
     CDS             14982..15563
                     /gene="sodB"
                     /locus_tag="IT904_002923"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAP3192676.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
BASE COUNT         3630 a         4122 c         4053 g         3816 t
ORIGIN      
        1 ttggcctgta actcaatgat attatgctca agttttgcga tgagcgtaat cagttgctcc
       61 agttcctctg cggagatgcc atgtaatatt tccgcgcggg ttttgttaat aacagcttcc
      121 atttcgctga tcagcggctc tgccttttcc gtcagtttaa tacgtttagc ccgacgatcg
      181 ctggcacaag tttgacgcga aattaaccct ttttcttcca gttggtccag agtacggacc
      241 agtgatggct gctcgatgcc aatcgctttt gccagttgaa tttgcgactg gtctggaggt
      301 aactgatgga tattgtgtaa cgtaacccaa tgggtttgtg ttaactccag cggtttcagg
      361 cgatggtcta tcagagcacg ccatatgcgc accaaccgtg ccagatcaga acctagtggc
      421 gattccaatt tcatctcctt ataattagct tgctaagata ttatgcggct tttagaatag
      481 tgtgcagcaa ttgtattgct aaaacaaatg tattgctgca tttggttacc gtcagacata
      541 tttttcagaa attgcgcgta aatttttcgc acttaaagaa tatttattaa tctaacgcaa
      601 tatattcggt cgtaaaggaa tctactttgt gaagtttatg ctcaatgcaa caggattgcc
      661 cttacaagat ctggtgttcg gtgcgtccgt ctactttcct ccgtttttca aagccttcgc
      721 gtttggattc gtcatctggc ttgtcgtaca ccgcctgctt cgtggctgga tctacgccgg
      781 tgacatctgg catcccttgt taatggattt atcgctgttt gcgatttgcg tttgccttgc
      841 tctggcaata ctgattgcgt ggtaactatg tcaattaaaa caattaagta tttctcaaca
      901 atcattgtag cggtagttgc ggttcttgcc ggatggtggc tgtggaatta ttacatgcaa
      961 tctccgtgga cacgcgacgg gaaaatacgc gcagaacagg tttctatcac cccacaggtg
     1021 tcgggacgta ttgttgagct gaatataaaa gacaaccagc tggttaacgc gggggatctt
     1081 ctgctcacga tcgataaaac gccctttcag atcgccgagc tgaacgcaca ggctcagctg
     1141 gcgaaagcgc aatctgacct cgcaaaagcc aataacgaag cgaatcgtcg tcgtcatctc
     1201 tcacaaaatt ttatttctgc cgaagaactc gataccgcta acctcaatgt taaagcgatg
     1261 caggccagtg ttgatgccgc acaggcgacg ctgaaacagg cacaatggca actggcgcaa
     1321 acggaaattc gcgccccggt gagtggatgg gtgactaacc ttactacccg catcggcgat
     1381 tacgctgata ccgggaaacc gctgtttgcc cttgtcgaca gccactcgtt ttacgtcatt
     1441 ggttattttg aagaaaccaa gttgcgccat atccgcgaag gtgcaccagc acaaattacc
     1501 ctctatagtg ataacaaaac gttacagggt cacgtttcca gtatcggtcg ggcgatttat
     1561 gatcagagcg ttgaaagtga ctcaagcctt attccggatg ttaaacctaa cgtcccctgg
     1621 gttcgactcg cccaacgcgt tcccgtccgc tttgcgctgg ataaagtccc aggcgatgtc
     1681 acgttggtgt cgggcaccac ctgtagcatt gccgtaggtc aataatgaac gcatcgtcat
     1741 ggtccttgcg caatttgccc tggttcaggg ccacgctggc gcaatggcgt tatgcgttac
     1801 gcaataccat tgccatgtgt ctggcgctga cggttgccta ttatttaaat ctggatgaac
     1861 cctattgggc gatgacctcg gctgcagtgg ttagctttcc caccgttggc ggtgttatca
     1921 gcaaaagcct cggacgcatc gctggcagtt tgctcggagc cattgcggca ctgcttcttg
     1981 ccgggcatac gctcaatgag ccgtggtttt ttctattgag catgtcggcg tggcttggct
     2041 tttgtacctg ggcctgtgcg cacttcacga ataacgtcgc gtatgcattt caactggcgg
     2101 gctacacggc tgccatcatc gcctttccga tggttaatat tactgaggcc agccagctgt
     2161 gggatatcgc tcaggcgcgc gtttgcgagg tgattgtcgg cattttgtgc ggcggcatga
     2221 tgatgatgat cctgcctagc agttccgatg ctacagccct tttaaccgca ttgaaaaaca
     2281 tgcacgcccg actacttgaa catgccagtt tactctggca gcctgaaaca accgatgcca
     2341 ttcgtgcagc acatgaaggg gtgattgggc agatactgac catgaatttg ctgcgtatcc
     2401 aggctttctg gagccactat cgttttcgcc agcaaaacgc gcgccttaat gcgctgctcc
     2461 accagcaatt acgtatgacc agtgtcatct ccagcctgcg acgtatgttg ctcaactggc
     2521 cctcaccgcc aggtgccaca cgagaaattc tcgaacagtt gctgacggcg ctcgccagtt
     2581 cgcaaacaga tgtttacacc gtcgcacgta ttatcgcccc gctacgcccg accaacgtcg
     2641 ccgactatcg gcacgtcgcc ttctggcagc gactacgtta tttttgccgc ctttatctgc
     2701 aaagtagtca ggaattacat cgtctgcaaa gcggtgtaga tgatcatacc agactcccac
     2761 ggacatccgg cctggctcgt cataccgata acgccgaagc tatgtggagc gggctgcgta
     2821 cattttgtac gttgatgatg attggcgcat ggagtattgc ttcgcaatgg gatgccggtg
     2881 ccaatgcatt aacgctggca gcaattagct gcgtactcta ctccgccgtc gcagcaccgt
     2941 ttaagtcgtt gtcacttctg atgcgcacgc tggtgttact ttcgctattc agctttgtgg
     3001 tcaaatttgg tctgatggtc cagattagcg atctgtggca atttttactg tttctctttc
     3061 cactgctggc gacaatgcag cttcttaaat tgcagatgcc aaaatttgcc gcattgtggg
     3121 ggcaactgat tgtttttata ggttctttta tcgctgtcac taatcccccg gtgtatgatt
     3181 ttgctgattt tcttaacgat aatctggcaa aaatcgttgg cgtcgcgttg gcgtggttag
     3241 cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag ccgccgccat attcgcgcgc
     3301 tgcgccggga ttttgtcgat cagctaagcc gccatccaac actgagtgaa agcgaatttg
     3361 aatcgctcac ttatcatcac gtcagtcagt tgagtaacag ccaggatgcg ctggctcgcc
     3421 gttggttatt acgctggggt gtagtgctgc tgaactgttc tcatgttgtc tggcaattgc
     3481 gcgactggga atcgcgttcc gatccgttat cgcgagtacg ggataactgt atttcactgt
     3541 tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc actggcggcc acacttgaag
     3601 aattacagcg gatttgcgac agccttgccc gtcatcatca acctgccgcc cgtgagctgg
     3661 cggcaattgt ctggcggctg tactgctcgc tttcgcaact tgagcaagca ccaccgcaag
     3721 gtacgctggc ctcttaatta cttaattaca ccacaggcat agcgttcacc gccaccgccc
     3781 agcggtttag gttgatcgga catattatcg ccgccaacgt ggaccatcag cgctttgtct
     3841 ttgatttcat ccagtgattt cagacgaggc gcgatgacgg catcggtagc tttgccgtca
     3901 ttattgacga ccagtgcagg cagatcgcct aaatgcccgg caccttctgg cccttcatgt
     3961 ttaccggtat tttgtggatc aagatgcccg cctgcggatt ccgcggcgct ggctttgcca
     4021 tctttggtgg ctggctggca gcttcctttg gcatgaatat ggaagccatg ttcaccgggg
     4081 ggtaatgctt tcagatcggg cgaaaactcc agacctttat cggtttcagt aatggtgacg
     4141 ctaccaattg actgccctac cccttgcgac gtgacgaggt tcatctcgac tttttcactg
     4201 gcagcttgtg cgccggttgc aacaaccagc gccagaatag ccagactaaa acgtttcata
     4261 ggacctccgt tcattgtgca ttcctgaata agtgtaaacc agtgacacat ttttgaacgg
     4321 cggctattcc taaaagtgct tacggtacgt cgtaccccag tgccgcttta cggatacgaa
     4381 accattgttg acgggtcatt ttcagtgttt ctgcttcgac agctgcccgt acgcgctcaa
     4441 ttttacctga accgataatt ggcagcggct gcgatggtaa acgtaatacc caggcgtaaa
     4501 ccacctgttc aatcgagccc gcgtttaact cctctgccac cacagccagt tcatcacgca
     4561 gcggctggaa ataatcatca ttaaacagac gaccaccacc aaggcaggac cacgccatcg
     4621 gacgaacacg cagttgttgt agttggtcga gcgtgccatc cagcagtaac ggctgatgca
     4681 ccggggatat ttccacctga ttagtggcaa gggtaaacgg cagacgtgat tgcaacaggg
     4741 caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg cactttgccg ctctgatgca
     4801 gatgtttgaa cgcgtccgcc acttcatcgg catccattaa cgggtctggt cggtggatta
     4861 acagcaaatc cagatgatcg gtcgcgagat taattagcga ctgttcggcg ctcttaatga
     4921 tgtgatcgcg gtcagtgatg taatgaccaa tgacgttttc ttcacgcgcg gtcgtcgcga
     4981 taccgcattt actgacgatt tccatccgtt cacgcaggtg aggtgccagt ttcagtgcct
     5041 cgccaaacgc cgcttcgcac tgatagccac cataaatatc agcatggtcc acggtggtca
     5101 cgccgagatc cagatgctct tcaataaaac tgaccagctg gcgggcggac atattccagt
     5161 ccatcaatcg ccagtagccc atcacaaaac gggaaaactc cgggccttgc ggcgcaatag
     5221 taatacgctg aaccataatc gcttcctctt atcagatatg agaggagtat acgcaagatt
     5281 aggttcaaaa gagtgatggt tgctccggtt cgtctgatga cgctggctta tttgcgcgta
     5341 atttgcgcat taatcgctgc cgacaaaggc gcagcacctc ttgtttttcg ccatcgctca
     5401 ttttattcca gttaaaacgc tcatcacgac tacgaaaaca gccgcgacaa aacccgcgtt
     5461 cgtcagactg gcaaattccc cggcacgggc tctggacggg aaagaactct aattgctccg
     5521 ccacttcgcc ctcctcagat aagattatta ccattattga agctgttaat gtccaaagta
     5581 gcaactttgc ttgcactaga ccgactggtc tactacactc caacgcatga acaaacacac
     5641 cgaacatgat actcgcgaac atctcctggc gacgggcgag caactttgcc tgcaacgtgg
     5701 attcaccggg atggggctaa gcgaattact aaaaaccgct gaagtgccga aagggtcctt
     5761 ctatcactac tttcgctcta aagaagcgtt tggcgttgcc atgcttgagc gtcattacgc
     5821 cgcatatcac cagcgactga ctgagttgct gcaatccggc gaaggtaact accgcgaccg
     5881 catactggct tattaccagc aaacactgaa ccagttttgc caacatggaa ccatcagtgg
     5941 ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg tcagaagata tgcgcagcgc
     6001 gatggataaa ggtgctcgcg gcgtgatcgc cctgctctct caggcgctgg aaaatggccg
     6061 tgagaaccat tgtttaacct tttgtggcga accgctgcaa caggcacaag tgctttacgc
     6121 actgtggctt ggcgcgaatc tgcaggccaa aatttcgcgc agtttcgagc cactggaaaa
     6181 cgcgctggcc catgtaaaaa acattattgc gacgcctgcc gtttagcagg cattttttat
     6241 caccagacga ccgggagcct ttatgtcatc tgaaaaactg tattccccac tgaaagtggg
     6301 cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg acgcgtctgc gcagtattga
     6361 accgggtgac atttctaccc cgttgatggc ggaatactat cgccaacgtg ccagtgccgg
     6421 tttgattatt agtgaagcca cgcaaatttc tgcccaggca aaaggatatg caggtgcgcc
     6481 tggcatccat agtccggagc aaattgccgc atggaaaaaa atcaccgctg gcgttcatgc
     6541 tgaaaatggt catatggccg tgcagctgtg gcacaccgga cgcatttctc acgccagcct
     6601 gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt agcgcgggaa cacgtacttc
     6661 tctgcgcgat gaaaatggtc aggcgatccg tgttgaaaca tccatgccgc gtgcgcttga
     6721 actggaagag attccaggta tcgtcaatga tttccgtcag gccattgcta acgcgcgtga
     6781 agccggtttt gatctggtag agctccactc tgctcacggt tatttgctgc atcagttcct
     6841 ttctccttct tcaaaccatc gtaccgatca gtacggcggc agcgtggaaa atcgcgcacg
     6901 tttggtactg gaagtggtcg atgccgggat tgaagaatgg ggtgccgatc gcattggcat
     6961 tcgcgtttcg ccaatcggta ctttccagaa cacagataac ggcccgaatg aagaagccga
     7021 tgcactgtat ctgattgaac aactgggtaa acgcggcatt gcttatctgc atatgtcaga
     7081 accagattgg gcggggggtg aaccgtatac tgatgcgttc cgcgaaaaag tacgcgcccg
     7141 tttccacggt ccgattatcg gcgcaggtgc atacacagta gaaaaagctg aaacgctgat
     7201 cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac tggattgcga acccggatct
     7261 ggtcgcccgc ttgcagcgca aagctgagct taacccacag cgtgccgaaa gtttctacgg
     7321 tggcggcgcg gaaggctata ccgattaccc gacgttgtaa tccaacattg cgagcggcgt
     7381 aaagccgccg ctatactaaa acaacatttt gaatctgtta gccattttga ggataaaaag
     7441 atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc aacgctccat cgatttttat
     7501 accaaagtgc tgggcatgaa actgctgcgt accagcgaaa acccggaata caaatactca
     7561 ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg tgattgaact gacctacaac
     7621 tggggcgtgg ataaatacga actcggcact gcttatggtc acatcgcgct tagcgtagat
     7681 aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg gtaacgtgac ccgtgaagcg
     7741 ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg aagatccgga cggttacaaa
     7801 attgagttaa tcgaagagaa agacgccggt cgcggtctgg gcaactaatc tcctgccggg
     7861 cgtgaactca tcgcgcccgc atctttactg catcgacaag taatatttgt cataatgcgc
     7921 gctgcaattt atccgtatta agagaatcag atgtccgata acgctcaact taccggtctg
     7981 tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg ttgaaacagc cggatttaac
     8041 gccaaaaccg atgcgctgct tgagattgcc gccatcaccc tgaaaatgga tgaacaaggc
     8101 tggctgatgc cggacaccac attacatttc cacgtcgaac catttgtcgg cgcaaatttg
     8161 caaccagaag ccctcgcctt caacggcatt gacccgaacg atcccgatcg cggcgcggtc
     8221 agcgaatacg aggcgctgca cgaaattttt aaagttgtac gtaaaggtat taaagcgagc
     8281 ggctgtaacc gcgccattat ggtggcgcac aatgccaatt ttgatcacag ctttatgatg
     8341 gccgccgcag aacgcgcctc actgaaacgt aacccgttcc accctttcgc cacttttgac
     8401 actgctgcac tggccgggct ggcactcgga caaaccgtat tgtcaaaggc ttgccagacc
     8461 gctggcatgg acttcgacag cacccaggcg cactccgcgc tgtacgacac cgaacgcact
     8521 gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc tgggaggctg gccgctacct
     8581 gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat gcaatgattc aggcatctat
     8641 agtgaggcta ttccacgcat cctgcatgat attcacgggg aatagcgtta atggcagata
     8701 atccagaccc ttcatcgctc ctgccggacg tgttttcacc ggcgacccgc gactggtttc
     8761 ttcgcgcctt taaacagccg accgctgtcc agccgcaaac ctggcatgtg gcggcgcgaa
     8821 gcgaacatgc gctggtgatt gcaccgaccg gctccgggaa aacgctggca gcatttctct
     8881 acgccctcga tcggctcttc cgcgaaggcg gcgaagatac ccgcgaggcg cataagcgta
     8941 aaacctcacg catcctctat atttcaccga taaaagccct gggcaccgac gttcagcgca
     9001 acttgcagat cccgttgaag ggtattgccg atgaacggcg gcggcgcggc gaaacggaag
     9061 tcaatcttcg cgtagggatc cgtactggcg atacgcctgc acaggaacgc agcaaactca
     9121 cccgtaatcc gccggatatt ctgatcacca cacccgaatc actctatctg atgctgacct
     9181 cccgcgcgcg cgaaacgcta cgcggcgtcg aaacggtaat tattgatgaa gtccacgcgg
     9241 tagcgggcag taaacgtggt gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc
     9301 tccacacctc agcacagcga attggccttt ctgccactgt gcgctcagcc agcgatgtgg
     9361 cagcatttct tggtggcgat cgcccggtta cggtagtcaa cccgcccgca atgcgccatc
     9421 cgcagatacg aattgtcgta ccggtcgcca atatggatga tgtctcatcg gtcgccagcg
     9481 gcaccggcga agacagccat gccggccggg aaggctccat ctggccatat attgaaacgg
     9541 gtatccttga tgaagtgttg cgccatcgct cgaccattgt ctttactaat tcacgcgggc
     9601 tggcggaaaa actgacggca cgattaaatg agctttacgc cgcacgctta cagcgttccc
     9661 cgtctatcgc cgttgatgcg gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg
     9721 tacaaagtag cgacgttttt attgcccgct cacaccacgg ctccgtctct aaagaacaac
     9781 gagcaatcac cgaacaggcg ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca
     9841 gtcttgaact ggggattgat atgggcgcgg tggatctggt gattcaggtg gcaacgccgc
     9901 tttctgttgc cagtgggtta caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat
     9961 ctaaagggct gtttttcccc cgtacccggc gtgatttagt cgattccgca gtcattgtag
    10021 agtgtatgtt cgcaggcagg ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc
    10081 ttgcgcagca aaccgttgcc gccgcggcga tggatgcatt acaggtagac gaatggtact
    10141 cccgcgtacg ccgtgccgca ccgtggaaag atctgccaag acgtgttttt gacgccacgc
    10201 tggatatgct ttccgggcgc tatccctctg gcgatttttc tgcttttcgc cccaaactgg
    10261 tctggaacag ggagaccggg atattgaccg cccgacctgg cgctcaattg ttggcggtta
    10321 ccagcggcgg caccattccg gatcgtggca tgtatagcgt gttattaccc gaaggtgaag
    10381 aaaaggccgg ttcgcggcgg gtgggtgaac tggatgagga gatggtatat gagtcgcggg
    10441 tgaacgacat tatcactctc ggcgctacct catggcggat ccagcaaatc acccgcgatc
    10501 aggtgattgt gactcctgct ccgggtcgtt ctgcccggct ccccttctgg cgtggtgaag
    10561 gtaacggacg tccggctgaa ttaggcgaga tgatcggcga ttttcttcat ttgctggcgg
    10621 atggcgcgtt cttttccggg actattcccc cgtggctggc agaagaaaat acgatcgcca
    10681 atattcaggg gttgattgag gagcagcgca acgcgacggg catcgttccg gggagtcgcc
    10741 atttggtcct cgaacggtgc cgtgatgaaa ttggtgactg gcgtattatt ttgcactctc
    10801 cctatggaag acgggtgcat gaaccctggg cgctggcgat tgccgggcga atacatgcgc
    10861 tatggggcgc tgacgcgtcg gtggtcgcca gtgatgacgg cattgttgca cgtattcctg
    10921 acaccgatgg taaattgccc gatgccgcga tttttttgtt tgaaccagaa aagttgctgc
    10981 aaattgtccg cgaggcggta ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg
    11041 ccgcgcgggc attattaatg ccggggcgca ctccgggcca tcgcaccccg ctttggcaac
    11101 aacggctgcg tgccagtcag ttgctggaaa tcgctcaggg atatccggat tttccggtca
    11161 ttctcgaaac cctacgcgaa tgtctgcaag atgtttatga tcttcccgca ctggaacgtt
    11221 tgatgcgtcg cctgaacggt ggcgaaattc aaatatccga tgtaacgacc actacgccct
    11281 cgcctttcgc cacaagttta ttgttcggct atgtcgcgga atttatgtac cagagcgacg
    11341 ccccgctggc agagcgccgg gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc
    11401 tactcggaca ggtcgatccg ggggaattac tcgacccgca ggtcattcgc caggtggaag
    11461 aagagttgca acgactggct cctggcagaa gagcgaaagg tgaagaagga ttgttcgacc
    11521 tgctgcgcga actggggcca atgaccgttg aagacctggc gcaacggcat acaggcagca
    11581 gtgaagaggt tgcgtcgtat ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga
    11641 tgattagcgg acaggagcgt cttgcctgta tggatgatgc cgccaggctg cgtgatgccc
    11701 tcggcgtacg actaccagag tcattgccag agatttattt acatagagtc agttacccgc
    11761 ttcgcgacct ctttctgcgc tatctccggg ctcatgctct ggtcacggct gaacaactgg
    11821 ctcatgagtt tagtctcggt attgccattg tcgaagagca gcttcagcaa ctgcgtgaac
    11881 agggtctggt gatgaatctg caacaagaca tctgggtgag cgatgaagta tttcgtcgtc
    11941 tgcgtttgcg ctcgctgcaa gccgccagag aagcgacgcg tcccgttgca gccacgacct
    12001 atgcgcgatt gctgctggaa cgtcagggcg tattacccgc caccgatggt agcccggcgc
    12061 tctttgcctc aacatcgcca ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg
    12121 aacagcttgc cggagtcggt ttacccgcct cactctggga aagccagatc ctgcctgccc
    12181 gcgtacgcga ctattcgtca gaaatgctcg atgaattact ggcaaccggt gcggttatct
    12241 ggtcggggca aaaaaaactg ggtgaagatg acggcctggt ggcactgcat ctacaggaat
    12301 atgctgcaga atcgttcact cccgccgaag cggatcaggc gaatcgttcg gcgctgcaac
    12361 aagcgatagt cgctgttctg gctgacggag gagcctggtt tgcacaacaa atcagccaac
    12421 ggatacgcga caaaatcggc gaatcggttg atctctctgc cctgcaagag gcgttatggg
    12481 cgctggtctg gcaaggcgtc atcaccagcg acatttgggc accgttacgc gccctcaccc
    12541 gcagcagttc caacgcacgc acctcaactc gccgcagtca ccgggctcgt cgtggacgtc
    12601 ctgtctatgc gcaacccgtc tcgccgcggg tatcttacaa cacaccaaat ctggctggac
    12661 gctggtcgtt attgcaggtg gagccactaa acgataccga aaggatgctg gcgctggcgg
    12721 aaaatatgct cgaccgctac ggcatcatca gtcgtcaggc ggtgatagcc gaaaatatcc
    12781 ctggcgggtt tccatcgatg caaacgcttt gtcgaagtat ggaagactcc gggcgaatta
    12841 tgcgaggtcg ttttgtagaa ggtctgggtg gcgcgcaatt cgctgaacgt ctgactattg
    12901 accgattgcg cgatctggcg acacaagcca cgcaaacgcg ccactataca ccagtggcgc
    12961 tctctgccaa cgatccggct aatgtgtggg gaaatcttct gccctggcct gcacatccgg
    13021 caacgctggt tccaacgcgt cgggcgggtg cgctggtggt cgtttctggc ggcaaattgt
    13081 tactctatct ggcgcaaggt ggcaaaaaaa tgctggtctg gcaggaaaaa gaggaattac
    13141 tcgccccaga ggttttccac gcgctgacta ccgcactgcg tcgcgaacca cggctgcgct
    13201 ttacgctaac agaagtgaat gatctaccgg tccggcaaac gccgatgttt acgctgctgc
    13261 gtgaggcggg attttcaagt tcgccacaag ggctggattg gggatagaga aaggactgac
    13321 ggatgcccgt tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag
    13381 acttgtattt agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt
    13441 cgatcacgat atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg
    13501 gccagttagc atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat
    13561 aggcaaaacg ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc
    13621 tcggcagttt cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt
    13681 ggattttttc gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat
    13741 ctgacattgt agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta
    13801 ttttatccct ttgctctatt tgttgcattt caaatattcg tttcttctct gatgccggca
    13861 aactggcgta tttataacca tttttagctt tcactgctgc atttttttgc gctcgccaac
    13921 gaaacgtatt ttttaacaat aaaagctatt aactttctct tcttctatgc attagaatca
    13981 tcaagttttg taaatcagac gcaggcatga tagacctgcc ttaacagagg gacgctcagt
    14041 ggcgcggata aaccgtattt cgatcacgct ctgtgcttta ctttttacca ccctaccttt
    14101 aacgcctatg gcccatgctt caaagcaagc cagggagagt tctgctacca ctcatatcac
    14161 caaaaaagca gataaaaaga aaagcacggc aaccaccaaa aagacccaga aaacagcgaa
    14221 aaaagccgcc agtaaaagta cgaccaaaag caaaaccgct tcttccgtta aaaaatcttc
    14281 cattaccgct tctaaaaacg ccaaaactcg cagcaaacac gccgtcaata aaacggcctc
    14341 agccagcttt accgaaaagt gtaccaaacg taagggctat aaatcgcatt gtgtgaaagt
    14401 caaaaatgcc gcgtcaggaa ctcttgccga cgcgcacaaa gcgaaggtgc aaaaagctac
    14461 gaaagtggca atgaataaac tgatgcagca aattggtaag ccatatcgtt ggggtggcag
    14521 ctcaccgcgt accggttttg attgcagcgg cctggtttat tacgcttata aagatttggt
    14581 gaaaattcgt attccgcgta cggcgaatga aatgtatcac ctgcgtgatg cagcgccaat
    14641 cgaacgtagt gaactgaaaa acggcgacct ggtctttttc cgtactcagg gacgcggcac
    14701 agccgatcat gtcggcgtgt atgtcggcaa cggcaaattt attcagtcac cgcgcacagg
    14761 tcaggaaatt caaatcactt ctctcagtga agactactgg cagcgccact atgttggcgc
    14821 tcgtcgggta atgaccccaa aaacacttcg ctaaaacttt accctgttgt tacggcaaca
    14881 gggtaagttc atcttttgtc tcacctttta atttgctacc ctatccatac gcacaataag
    14941 gctattgtac gtatgcaaat taataataaa ggagagtagc aatgtcattc gaattacctg
    15001 cactaccata tgctaaagat gctctggcac cgcacatttc tgcggaaacc atcgagtatc
    15061 actacggcaa gcaccatcag acttatgtca ctaacctgaa caacctgatt aaaggtaccg
    15121 cgtttgaagg taaatcactg gaagagatta ttcgcagctc tgaaggtggc gtattcaaca
    15181 acgcagctca ggtctggaac catactttct actggaactg cctggcaccg aacgccggtg
    15241 gcgaaccgac tggaaaagtc gctgaagcta tcgccgcatc ttttggcagc tttgccgatt
    15301 tcaaagcgca gtttactgat gcagcgatca aaaactttgg ttctggctgg acctggctgg
    15361 tgaaaaacag cgatggcaaa ctggctatcg tttcaacctc taacgcgggt actccgctga
    15421 ccaccgatgc gactccgctg ctgaccgttg atgtctggga acacgcttat tacatcgact
    15481 atcgcaatgc acgtcctggc tatctggagc acttctgggc gctggtgaac tgggaattcg
    15541 tagcgaaaaa tctcgctgca taataactga tggcaaatgc agcattgcct gaagcgctac
    15601 gcttatcagg cctacgcgga t
//
LOCUS       DACAPB010000105        15445 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.105, whole genome shotgun sequence.
ACCESSION   DACAPB010000105 DACAPB010000000
VERSION     DACAPB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.105"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..480)
                     /locus_tag="IT904_002924"
     CDS             complement(<1..480)
                     /locus_tag="IT904_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HAP3192677.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     "
     gene            712..1914
                     /locus_tag="IT904_002925"
     CDS             712..1914
                     /locus_tag="IT904_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP3192678.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVSDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(2230..3282)
                     /locus_tag="IT904_002926"
     CDS             complement(2230..3282)
                     /locus_tag="IT904_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HAP3192679.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            3665..5272
                     /locus_tag="IT904_002927"
     CDS             3665..5272
                     /locus_tag="IT904_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAP3192680.1"
                     /translation="MKAIDNQIRNISSSHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFVLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDIIYKVDGLSIIARNF
                     FTLLSGNFKPDWRWDVSKETFTKEKFDSYVRSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGI
                     IRDEEKKCYCLGALQVPFNQMKNSTDPEELANIFNKKHKEHMDDPLFHLIRSNTLKPT
                     PVYANYFDNTTDFRPARKRIFICGETLKGEDPSKYIRQKYGHHGVGVDQNQQFDNGIM
                     GLNSLKEARDKNNKIIYSSGLSCFQSH"
     gene            5534..7156
                     /locus_tag="IT904_002928"
     CDS             5534..7156
                     /locus_tag="IT904_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAP3192681.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSKKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLPNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALEPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            7522..8589
                     /locus_tag="IT904_002929"
     CDS             7522..8589
                     /locus_tag="IT904_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP3192682.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            8586..11321
                     /gene="rbbA"
                     /locus_tag="IT904_002930"
     CDS             8586..11321
                     /gene="rbbA"
                     /locus_tag="IT904_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAP3192683.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLIIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            11321..12445
                     /locus_tag="IT904_002931"
     CDS             11321..12445
                     /locus_tag="IT904_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAP3192684.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDTMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(12610..13746)
                     /locus_tag="IT904_002932"
                     /pseudo
     CDS             complement(12610..13746)
                     /locus_tag="IT904_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(13992..14252)
                     /locus_tag="IT904_002933"
     CDS             complement(13992..14252)
                     /locus_tag="IT904_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192685.1"
                     /translation="MEVPAMSNTYQKRKASKEYGLYNKCKKLNDDELFRLLDDRNSLK
                     RISSARVLQLRGGQDAVRLAIEFCTDKNYIRRDIGAFILGQI"
     gene            complement(14342..14725)
                     /gene="yhhH"
                     /locus_tag="IT904_002934"
     CDS             complement(14342..14725)
                     /gene="yhhH"
                     /locus_tag="IT904_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HAP3192686.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            complement(14697..15407)
                     /locus_tag="IT904_002935"
     CDS             complement(14697..15407)
                     /locus_tag="IT904_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HAP3192687.1"
                     /translation="MLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYIT
                     QDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDS
                     IDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNG
                     TEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGK
                     EITNRKGLPNVCRVHGNM"
BASE COUNT         4170 a         3501 c         3613 g         4161 t
ORIGIN      
        1 gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca ccactgacgt
       61 cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga
      121 gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg ccgccagcaa
      181 catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca gatccgtcgg
      241 cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac ccaaaaagtt
      301 gaacaccgcc gccataacca cggcgagctg agaacgcatc gcgcgggtat agataacggt
      361 tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca gcacaaaagc
      421 cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca aatgtagcat
      481 aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc
      541 gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatttc actcaaaaaa
      601 taattatctt atataattca ggcaaatact tccttttagt aatattgatg ctggtgcgac
      661 cactgaggaa tctttacaat tcacgcccgt tttttctaag aggagcgcaa cgtggaaagg
      721 tttgatgcca ttattatagg cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt
      781 caggcaggac gccgggttct gctgatcgat aatggtaaaa aaccagggcg caaaatcctt
      841 atgtctggcg gtgggcgctg caactttacc aacctttatg tcgaaccagg cgcttatctg
      901 agccagaatc cgcatttttg taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt
      961 gatctggtca ataaacacgg catcgcctgg cacgagaaaa cgttaggaca actcttctgc
     1021 gatgactccg cgcagcagat tgtcgacatg ctggtggatg agtgcgagaa gggcaatgta
     1081 accttcagat tgcgtagcga agtgctgagt gtggcgaagg atgaaacagg cttcacgctt
     1141 gaactgaacg gcatgactgt cggttgcgaa aagctggtca tcgcgaccgg tgggctgtca
     1201 atgccggggc tgggcgcgtc gccgtttggt tataagattg ccgaacaatt tggcctcaac
     1261 gtgctgccga cccgtgcggg tctggtgcca ttcactctgc ataaaccgtt gctcgaagag
     1321 ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta ccgctgaaaa cggcatcgtt
     1381 ttccgtgaga acttactctt cacccatcgc ggcttgtctg gaccggcggt gttgcagatt
     1441 tcaagctact ggcaaccggg tgaatttgtc agtatcaatc tgctacctga tgtggacctc
     1501 gaaaccttcc tgaatgagca gcgtaacgca catccgaatc agagcctgaa aaacacactg
     1561 gcggttcatc taccgaagcg gttggttgaa cgcttacagc aactcgggca aatcccggat
     1621 gtttcgctaa aacagctcaa cgtgagtgac caacaggcac tgattagcac attgaccgac
     1681 tggcgcgtac aacccaacgg cactgaaggc tatcgcactg ccgaagtgac gctcggcggc
     1741 gtggacacca acgaactctc ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac
     1801 ttcatcggcg aagtgatgga cgtcaccggc tggctggggg gctataactt ccagtgggcg
     1861 tggtcgagtg catgggcttg tgcgcaggat ttgattgcag cgaagtcgtc ctgacctgtt
     1921 acctaaagat gaaaattatt agagccgcat taaaaatgag ctaattttga tagtggctat
     1981 cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct ccaggcttaa tataaggtgg
     2041 aaggaaaggt gattgaaaac tcacccagtg gccagatctt tataaaatat gaacaggctg
     2101 aaaaagacag taactttgtt gtttttttat ccttaaccac tatgcattaa tgctgcgtta
     2161 tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt tttatccggt
     2221 acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg agaacaacgt
     2281 aaagcatacc gctcccaaac cgaccaaaac ccgcgaagga ataaaatatc ccatgtcggt
     2341 ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg tgaagacggg
     2401 aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg ccagtagcca
     2461 gacttttgag aatatgctgt aacagatcat tccgaggcaa ataagaataa tgccgggagc
     2521 cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta ccgtgattga
     2581 gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa acgtattacg
     2641 tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg cggttaaccc
     2701 aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta acagcgtaat
     2761 agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc agttaccgat
     2821 taatgaagaa taagcctgta ccggtgttcc gtcgctcttg ctccccgctg catttttggg
     2881 aatgagcaga aagtgaccgg atgacgctgc caccgtcgat acacaggcgg caatcatacc
     2941 aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc atcacatcat tgcctgctaa
     3001 cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa tggggaacaa
     3061 tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa atgcggtagt
     3121 gaataatgcc aggcatatgg ccgccagaga aatcagcaca tgacccgcga cgaaatactc
     3181 gctttgccct gaactttcca gcacaaagcc acctaacgcg atacaaatta agcccataat
     3241 caaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact cctcaggtat
     3301 ccctgctaat attgaaatat gcattaatga taatttcatc ctgttaatat aatgacgaga
     3361 tatcattaaa gacaaatgca tagcataact atagcggggt ttactttgca gagcgctact
     3421 tttcagcgtt caaaaggcca tgataaataa aaatttatcc taagattaat tcttatgctg
     3481 ttgtttttta caattatctt ccagggaggg attattcttc attatttggt aaaagaccgg
     3541 atagtctcgg tgcttaaaat cagcgaacat ataagtatta ttattttata tcgtcgtaaa
     3601 tctgtttttc agacaagtat catttatttt gttacaaaca attcaaaaat atcacaggtt
     3661 atttatgaaa gcaattgaca atcagattag aaacatctct tcaagccatc aagataagca
     3721 ttcagataaa gttaacagtc atcaacacca tggcaaggtt gataaaacac atcgtgcaaa
     3781 gatagttgaa tttgataaac tggataatga ttcgcaaata gataacgatt ttgtcctgca
     3841 tatcatctat ttcctgcaac atggtcactg gaaggtaaat gatcgtagtc accagatgga
     3901 gaaagtatgg ttttacaata gtgaaccttc tatagatatt caagaatata atagattcgc
     3961 cgacaatact actgatacgt ttatctttac aattatacca gacaataacc atgtgttaaa
     4021 attatcgtct cccattaccg ttactgttga gtgtaagggc ggttattatt ttattaattc
     4081 ctctggagat aaatcagata taatttataa agttgatggg ctttctatca tagcgagaaa
     4141 tttctttact cttcttagtg gtaatttcaa acctgactgg cgttgggatg tctctaagga
     4201 gacgtttact aaggaaaagt ttgatagcta tgttagatct gtgttttcaa aaatagactt
     4261 ctataaacag tgtggtgtga ttaacccaca aaatgccaac acggcttatt ttggcgatac
     4321 agatggaaga gtaggcgctg tactttatgc attgctcgtt tcagggcata taggcatcag
     4381 ggaaaagggt tggagtttat tatgtgaatt attaaaacac gaagagatgg cttcatctgc
     4441 atacaaacat aaaaataata aagtgctcta cgatttatta aatacgagag acatgatctt
     4501 gaatgagttg catcaacatg tttttttaaa ggacgatgcg ataacccctt gtatcttcct
     4561 tggggatcat acaggcgata gatttagtac tatttttggt gataaatata tccttacttt
     4621 gctaaattct atgcgaaata tggagggtaa taaagatagc cgtattaata aaaatgtcgt
     4681 cgttctggct ggaaatcatg aaatcaattt taatggtaac tatactgcac gactagcaaa
     4741 tcataaactc tctgccggtg atacgtataa cttaattaaa acgctcgatg tttgtaatta
     4801 tgactcagaa aggcaagttt taaccagtca tcacggaata ataagagatg aagaaaaaaa
     4861 atgttattgt ctcggtgctt tgcaggttcc atttaatcag atgaaaaatt ctactgatcc
     4921 tgaggaatta gccaatattt ttaataaaaa acataaagaa cacatggatg atcctctatt
     4981 ccatctaatc cgaagcaata ctttgaaacc cacacctgta tatgctaatt attttgataa
     5041 cactactgat ttcagaccag cacgtaaacg tatcttcata tgtggggaga cactcaaagg
     5101 tgaagacccc agcaaataca ttagacaaaa atatgggcat catggggtcg gggtagatca
     5161 gaatcaacaa tttgataatg gtattatggg gctaaattcc ctgaaggaag cacgtgataa
     5221 aaataataaa ataatttatt cctcagggct tagttgtttt caatctcatt aaaactccat
     5281 gaatacggtg gtatgtcttt ttaatcctgc cagtatttca taggttttat taatcgattg
     5341 tagaggtatg tactgatgac gcctctacaa tctttaggga ctttctctgc tatgagataa
     5401 gaaatttcat attatgcttt aaggctgaat gatttgtgca aaatataaat atttttccat
     5461 cgaatatgag gtgtatgtca taagataaaa tgttaactat gtattcctcg cggtgaataa
     5521 tatgaggtaa aagatgaaaa ttggaactgt ggcaggcact aacggcagta caacgacaat
     5581 cgcaacaaat gatatggtgc aagagcatgt taccaacttc actaaagaac tgtttgggta
     5641 tattgcaaat ggtataggtg atgatatatc aagtattgcc aggacaatgc tcggtgaagt
     5701 tgtagaaaaa attgatgatt ggcaaataga acgtttccaa caatctattc aagatgacaa
     5761 aatttcattt accatacaaa cgaatcattc aaaaaaatat agtatgctat cgggcatgcg
     5821 cgctcatatt ctacgtaggg ataataatta tcagtttatt gtgacaatta atagtataaa
     5881 ttacggctgc tatcttgata ataccgatat taattggtgc agcattgttt atttacttaa
     5941 taatatgaca gtaaatgatg atgctaatga tgttgcagtt actgagtcgt acaaacccat
     6001 ctggaactgg aaaatatcgc aatacaatgt atttgatatt aaattcgaaa caatgataaa
     6061 accccagttc gctgacagaa cctatttctc caattgttca cctgttgatc ccacaagtac
     6121 caggccgacc tattttggcg ataccgatgg ctcagtcggt gcagttttgt acgcgctttt
     6181 tgcaacggga catttgggaa tcatggctga aggagaaaat ttccttagcc agttgcctaa
     6241 tatagaagat gaagttctca acgtattgct aagggagaac tttaatgagc aacttgacac
     6301 taatgttaat ataataatca gtatcctgaa cagaagagac attgttttag aaagtcttca
     6361 gccttatttg gttattaata aagacgcagt cacgccatgt acgtttctgg gcgaccagac
     6421 tggcgatcga tttagtaata tctgtggcga taaatttatc atcgatttat taaaacgcat
     6481 aatgagtatc aatgaaaacg ttcatgtatt agctggaaat catgaaacga attgcaatgg
     6541 taactatatg cagaacttca cgcgcatgaa accacttgat gaggatacct actctgggat
     6601 aaaagattat cctgtatgtt tctatgaccc caaatacaaa ataatggcaa atcatcatgg
     6661 gataactttt gatgatcagc gaaaacgtta cattatagga ccgataacag tctctagtga
     6721 tgaaatgacc aatgctctcg aaccggtcga attagcggca attatcaata aaaagcacca
     6781 tgctgtaatt aatggcaaaa agtttaaaaa tagccgggct atctcttgtc gatcctttaa
     6841 tcgttatttt tctgtttcga cagattacag acctaaactt gaagcactat tagcatgctc
     6901 acaaatgtta ggtataaatc aagttgttgc acataacggt aatggtggaa gagaatgtat
     6961 aggtgaaacg gggacagttt taggtcttaa cgcccgtgac agtaaacatg ctggaagaat
     7021 gttcagcatg cataattgcc aaattaaccc tggtgcaggg cctgagataa ccactccctg
     7081 gaagtcttac cagcatgaaa aaaacagaaa tggacttatg cctttgatca gaaggcgcac
     7141 aatgttacag ctctgaacca tagacaacat gaatattaat gttactatct ggttgagact
     7201 gttgataaaa cgtaaaaagg aatgctctgt tcctgcaact gaacaggcgt gaaacgctat
     7261 ccaacaggat ggataccgtt ttgtcagcca gttttataga tcacacttat gagtatgcgt
     7321 gtttcattta cggccaggaa tgctgatgat gacaacatta ctacccgttt tcactaagcc
     7381 ttccccgttg gcgctcaatg ctctgcgcgc tggtcgtatt tgtcgtttcc ttcttatccc
     7441 ggatggaaga atccgctgaa cgtcgttcgg cggcagttgt aacacgcgtc gtttgcttac
     7501 agggattatg gcgggtcgat tatggataag agtaagcgcc atctggcgtg gtgggttgtc
     7561 gggttactgg cggtggcggc tatcgtggcg tggtggctgt tgcgcccggc aggtgtgccg
     7621 gaaggctttg ccgtcagcaa tgggcgcatt gaagcgacgg aagtggatat cgccagcaaa
     7681 attgccgggc gtatcgacac cattctggtg aaagaaggcc agtttgttcg cgaaggcgaa
     7741 gtgctggcga agatggatac tcgcgtgttg caggaacagc gactggaagc catcgcgcaa
     7801 atcaaagaag cacaaagtgc cgttgctgcc gcgcaggctt tgctggagca acggcaaagc
     7861 gaaactcgtg ccgcgcagtc gctggttaat caacgccagg ccgaactgga ctccgtggct
     7921 aaacgtcata cgcgttcccg ctcgctcgcc caacgagggg ctatttctgc gcaacagctg
     7981 gatgacgatc gcgccgccgc tgagagcgcc cgagctgcgc tggaatcggc gaaagctcag
     8041 gtatcggctt ctaaagcggc tatagaagcg gcacgcacca atatcattca ggcgcaaact
     8101 cgcgtcgaag ccgcacaagc cactgaacgg cgcattgccg cagatatcga tgacagcgaa
     8161 ctgaaagccc cgcgtgatgg acgcgtacaa tatcgcgttg ccgagccagg ggaagtgctg
     8221 gcggcaggcg gtcgggtgct gaatatggtc gatctcagcg acgtctatat gactttcttc
     8281 ctgccaaccg aacaggcggg cacgctgaaa cttggcggtg aagcccggct gatcctcgat
     8341 gccgcgccag atctgcgtat tccggcgacc atcagttttg tcgccagtgt cgcccagttc
     8401 acgccaaaaa ccgtcgaaac cagcgatgaa cggctgaaac tgatgttccg cgtcaaagcg
     8461 cgtatcccac cggaattact ccagcagcat ctggaatatg tcaaaaccgg tttaccgggc
     8521 gtagcgtggg tgcgggtgaa tgaagaactt ccgtggcctg acgacctcgt ggtgaggttg
     8581 ccgcaatgac gcatctggaa ctggttcccg tcccgcctgt cgcgcaactg gcgggcgtga
     8641 gccagcatta tggaaaaacc gttgcgctga acaatatcac tctcgatatt ccggcccgtt
     8701 gcatggtcgg gctgattggt ccggatggcg tcgggaagtc gagtttattg tcactgattt
     8761 ccggtgcccg cgtcattgag cagggcaatg tggtggtgct gggtggcgat atgcacgacc
     8821 cgaagcatcg ccgagacgtc tgcccgcgca tcgcctggat gccgcagggg ctgggcaaaa
     8881 acctctacca caccttgtcg gtgtatgaaa acgtcgattt tttcgctcgc ctgttcggtc
     8941 acgacaaagc ggagcgggaa gtgcgaatta atgagctgct gaccagcacg gggttagcac
     9001 cgtttcgcga tcgtccggca gggaaactct ccggcgggat gaagcaaaaa cttgggctgt
     9061 gctgcgcgtt aatccacgac ccggaactgt tgatccttga tgagccaaca acgggggttg
     9121 acccgctctc ccgctcccag ttctgggatc tgatcgacag tattcgccag cggcagagca
     9181 atatgagcgt gctggtcgcc accgcctata tggaagaggc cgaacgcttc gactggctgg
     9241 tagcgatgaa tgccggagaa gtgctggcaa ctggcagcgc cgaagagcta cggcagcaaa
     9301 cgcaaagcgc tacgctggaa gaagcattta taaatctgtt accgcaagcg caacgccagg
     9361 cgcatcaggc ggtagtgatc ccaccgtatc aacctgaaaa cgcagagatt gccatcgaag
     9421 cgcgcgatct gaccatgcgt tttggttcct tcgttgccgt tgatcacgtt aatttccgca
     9481 ttccacgcgg ggagattttt ggttttcttg gttcgaacgg ctgcggtaaa tccaccacca
     9541 tgaaaatgct caccggactg ctgcccgcca gcgaaggtga ggcgtggctg ttcgggcaac
     9601 cggttgatcc aaaagatatc gatacccgcc gtcgggtggg ctatatgtcg caggcgtttt
     9661 cgctctataa cgaactcacc gtgcggcaaa accttgagtt acatgcccgt ttgtttcaca
     9721 tcccggaagc ggaaattccc gcaagagtgg ctgaaatgag cgagcgtttt aagctcaacg
     9781 acgttgaaga tattctgccg gagtcattgc cgctcggcat tcgccagcgg ctttcgctgg
     9841 cggtggcggt gattcatcgc ccggagatgt taatcctcga tgagcctact tctggtgtcg
     9901 atccggtggc gagggatatg ttctggcagt tgatggtcga tctctcgcgc caggacaaag
     9961 tgactatctt catctccacc cactttatga acgaagcgga acgttgcgac cgcatctcac
    10021 tgatgcacgc cggaaaagtg cttgccagcg gtacaccgca ggaactggtt gagaaacgcg
    10081 gagccgccag tctggaagag gcatttatcg cctatttgca ggaagcggca gggcagagca
    10141 acgaagccga agcgccgccc gtggtacacg acaccaccca cgcgccgcgt cagggattta
    10201 gcctgcgccg tctgtttagc tacagccgcc gcgaagcgct ggaactgcga cgcgatccag
    10261 tacgttcgac gctggcgctg atgggaacgg tgatcctgat gctgataatg ggttacggca
    10321 tcagtatgga tgtggaaaac ctgcgctttg cggtgctcga ccgcgaccag accgtcagta
    10381 gccaggcgtg gacactcaac ctctccggtt cccgttactt tatcgaacag ccgccgctca
    10441 ccagttatga cgagcttgat cgtcggatgc gtgcgggcga tatcacggtg gcgattgaga
    10501 tcccgcccaa tttcgggcgc gatatcgcgc gtggtacgcc tgtggaactc ggcgtctgga
    10561 tcgacggagc gatgcccagt cgtgccgaaa cggtaaaagg ttacgtgcag gcaatgcacc
    10621 agagttggct tcaggatgtg gcgagccgac aatcgacacc cgccagccaa agcgggctga
    10681 tgaacattga gacgcgttat cgctataacc cggacgtaaa aagcctgcca gcgattgttc
    10741 cggcggtgat cccgcttctg ctgatgatga tcccgtcaat gctaagcgcc cttagcgtgg
    10801 tgcgggaaaa agagcttggg tcgattatca acctttacgt gacccccacc acgcgtagtg
    10861 aatttttgct tggtaaacag ttgccataca tcgcgctggg gatgctgaac tttttcctgc
    10921 tctgcggcct gtcggtgttt gtgtttggcg taccgcataa aggcagtttc ctgacgctca
    10981 ccctggcggc gctgctgtat atcatcattg ccaccggaat ggggctgctg atctccacct
    11041 ttatgaaaag ccagattgcc gccattttcg gaacggcgat tatcacgttg atcccggcga
    11101 cacagttttc cgggatgatc gatccggtag cttcgctgga agggcctgga cgttggatcg
    11161 gcgaggttta cccgaccagt cattttctga ttatcgcccg cgggacgttc tcgaaagcgc
    11221 tggatctgac tgatttgtgg caacttttta tcccgttgct gatagccatc ccgctggtga
    11281 tgggcttaag tatcctgctg ctgaaaaaac aggagggatg atgcgccatt tacgcaatat
    11341 ttttaatctg ggtatcaaag agttgcgcag tctgctcggt gataaagcga tgctgacgct
    11401 gattgtcttc tcgtttacgg tgtcggtgta ttcgtcagcg accgttacgc caggatcgtt
    11461 gaacctcgcg ccgatcgcca ttgccgatat ggatcaatcg cagttatcga accggatcgt
    11521 taacagcttc tatcgtccgt ggtttttgcc accggagatg atcaccgccg atgagatgga
    11581 tgctggactg gacgccggac gctatacctt cgcgataaat attccgccta attttcagcg
    11641 tgatgtcctc gccggacgcc agccggatat tcaggtgaac gtcgatgcca cgcgcatgag
    11701 ccaggcattt accggcaatg ggtatatcca gaatattatc aacggtgaag tgaacagctt
    11761 tgtcgcgcgc taccgtgata acagcgaacc gttggtatcg ctggaaaccc ggatgcgctt
    11821 taacccgaac cttgatcccg cgtggtttgg cggggtgatg gcgatcatca acaacattac
    11881 catgctggcg attgtattga ccggatcggc gctgatccgc gagcgtgaac acggcacggt
    11941 ggaacattta ctggtgatgc cgataacgcc gtttgagatc atgatggcga agatctggtc
    12001 gatggggctg gtggtgctgg tggtatcggg attatcgctg gtgctgatgg tgaaaggtgt
    12061 actgggcgta ccgattgaag gctcgatccc gctgtttatg ctgggcgtgg cgctcagtct
    12121 gtttgccacc acgtcaatcg gcatttttat ggggacgata gcgcgttcaa tgccgcaact
    12181 ggggctgctg gtgattctgg tgctgctgcc gctgcaaatg ctttccggtg gatccacgcc
    12241 gcgcgaaagt atgccgcaga tggtgcagga cactatgctg accatgccga cgacacactt
    12301 tgttagcctc gcgcaggcca tcctctaccg gggtgccgga ttcgaaatcg tctggccgca
    12361 gttcctgacg ctgatggcaa ttggcggcgc gtttttcacc attgcactgc tgcgattcag
    12421 gaagacgatt gggacaatgg cgtaaataca attgcccgat gcgtaagctt atagggacta
    12481 cgcatcggtg caatttattg aattttagtt gtttttaggc cggataaggc gtttatgaac
    12541 tgacccccaa aacctggaca gtttcagtta accggctttt tgatactcag tcctgtattc
    12601 agggcaagat tacgaaagcc cgctccccgc aaggactgac gccagatagt ttctgtccat
    12661 ggctgctttt cgcatcttac gtcttaaccc tgccttgaat accttatcat tcgtcaaaat
    12721 attaatagca atgtgccgta tccctgaaaa taattctgct gcatttcctc ttcttatttt
    12781 gcagtcgtct tcattcatta ccacgtccag acgccagtgc agcttattct ccacgtgcca
    12841 gtggtttcgg attgctgtgg cgaacttctc tgcggttaaa tcagcagaac tgatataata
    12901 tctgaccgtc atttctggct ctttcttttg ttctgctatt atggaccgaa aggagactgc
    12961 cacgcataat ttcttcagcc ctttccattc aaacgtgaaa tcaataagtt catcagggac
    13021 atcgcaaaca atatgaagac ggatttcttc tctgccgtga ctcttttcac tcattgcgta
    13081 actgtcatgc tctggattat ttaattcttt cagcggaaat ttttcctcaa aggctttatt
    13141 tagccgcccc tggtttcctt ttaccgcgaa taaataatta cctccctgtt tttgtatctt
    13201 ttctgcaata tctttctggc agcccatcgc atcagttgtg atgatttttc ctttaatatc
    13261 catcatgtta agaagttcag gaatggctgt aatctcattg gatttctcat ccgtcttgat
    13321 ctgtccgagg accagactat gcattgttga gaacgcacta atgacatgaa tcgctcccct
    13381 gcgacgactc ttgtcataag agtgccggag cgtttttcca tcaattgcaa tgacgtcttt
    13441 atcatctgaa gaatggcagt cacgcatcca gttaataaag cactcgtgaa attttgcagg
    13501 actgatacag gatacaactc tggcaatgat atcgtgaaca ggaataccat tttcaaaatc
    13561 accatatcac ttcaaaacat cgggatgtat ttccccaaaa tcctctatat cttcccaacc
    13621 ttctgcacca gaaataacgg cacaaatagt caacagtaga atatccgata atttatgttc
    13681 cactttccag gtttgtctgt aatcggggat aatagaaata tgttccatca attttttaag
    13741 ttccattttg ttctccttaa ttaggtaagg agtatttgat catgtataag caataaaaaa
    13801 cagcttcagg taataaggaa tatctcaatt tttaaacata aaatgcgaat tatttagtgc
    13861 aaaaagtgaa cttttgatga tctttccctg gccgttgact cgattgccgt agctcgaacg
    13921 caagcactct tatcattcaa tgccatattg ttcaaaatat taaaaacatt atcttcgcat
    13981 tttttgcaaa tttatatttg cccgagtata aatgctccga tatctctacg gatataattt
    14041 ttgtcagtgc agaattcaat tgccaatcta acagcgtctt gcccccctct taactgtaat
    14101 actctggcag atgaaatcct tttcaaggaa ttgcgatcat caagtaagcg aaataattca
    14161 tcatcattta gtttcttaca tttattatat aaaccatact ctttacttgc ctttcttttc
    14221 tggtatgtat ttgacattgc aggaacctcc ataaaggcta tctttttcta tcgtttgact
    14281 gtctggagtc ctccacctac tctgccatca gcgcacacga tgtttgatta tccttacata
    14341 cttattttcc gtggccaaaa ttcagaattc tcccatcatt tttgcctatt tctataataa
    14401 aaaccccacc agcaatttgg tctgagtgaa tggtaccttc aacaacccaa cttgttgtta
    14461 actcagtgat ttcgtatggt ttttcttctt ctgcggcatc ctgtccataa cgattcttaa
    14521 catatatctc agctaattcc aatgctatag ctttattttt aaccaaattt ctaacggtat
    14581 atttaccttc actttcatac ttgcgcatta ttttaatatt attattacac tgcgcccatg
    14641 tgttgtatgt aaaaacaaac aaagatacaa tgacacagtt cctaaacaat atttttttac
    14701 atattaccat gaactctgca gacattaggt aatccttttc tgtttgttat ttccttcccg
    14761 tggttatcta ttgctttaaa attaccactg ggcgtaccta aataaaatga cttgatattg
    14821 ttatctttac ttttaactat tttctcatct ttacctgaaa atatttcgtc ccaatattct
    14881 ccatgactat ctgcaccatg agtatgataa gctgatactt tctcagtacc attagggcaa
    14941 gggctattga atggatatga tccttttctt tctcctcggt taggtgctgt tgagaaatat
    15001 ctgccactat tatccttaca tattaaacca gcgaactctt tatcttcgca aatagattgg
    15061 ctatttattt tatttaaggc gtcatatgcg gtgctatcta tagaatcaaa tttatctgga
    15121 cctatcatag cggatagtgt gccgcaaaga ccattagtac atgcccccga ttttgcatcc
    15181 tcccacatag ataaacccag ggggtcgatg tctgatatcg gattcaattg atatccatac
    15241 aggttccatc cccccttcag tccaatcgga tcctgagtga tatatcgccc ctgcagcggg
    15301 tcataatagc ggtggcggtt gtaatacagg ccggactcct catcatactg ctgccccggc
    15361 aggcggataa gctgctgtag ctgatgcggg ttctcttcat tcagcaggtt gccccactca
    15421 tcgtattctg cgcaccattc tgttg
//
LOCUS       DACAPB010000106        15360 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.106, whole genome shotgun sequence.
ACCESSION   DACAPB010000106 DACAPB010000000
VERSION     DACAPB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.106"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1467
                     /locus_tag="IT904_002936"
     CDS             <1..1467
                     /locus_tag="IT904_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HAP3192688.1"
                     /translation="VYAPTGATLTATLTSANGTPVEGQVINFSVTPEGATLSGGKVRT
                     NSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSSTAHVASFIADPSTIA
                     ATNTDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAM
                     TGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGDYTDSAELRLVLHDI
                     SGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTGGGEGIATLIPVLNG
                     VHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKT
                     AADYEFSSSASWVDVDATGKVTFKNVGSNWERITATPKSGGPSYVYEIRVKSWWVNAG
                     DAFMIYSLAENFCSSNGYTLPRADHLNHSRSRGIGSLYSEWGDMGHYTTDAGFQSNMY
                     WSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNI"
     gene            complement(1730..2782)
                     /locus_tag="IT904_002937"
     CDS             complement(1730..2782)
                     /locus_tag="IT904_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAP3192689.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVMSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCFSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDMPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            3097..4413
                     /gene="shiA"
                     /locus_tag="IT904_002938"
     CDS             3097..4413
                     /gene="shiA"
                     /locus_tag="IT904_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HAP3192690.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            4515..5969
                     /gene="amn"
                     /locus_tag="IT904_002939"
     CDS             4515..5969
                     /gene="amn"
                     /locus_tag="IT904_002939"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAP3192691.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            6312..7028
                     /locus_tag="IT904_002940"
     CDS             6312..7028
                     /locus_tag="IT904_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP3192692.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(7483..7558)
                     /locus_tag="IT904_002941"
     tRNA            complement(7483..7558)
                     /locus_tag="IT904_002941"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7523..7525),aa:Asn,seq:gtt)
     gene            complement(7743..9146)
                     /gene="yeeO"
                     /locus_tag="IT904_002942"
     CDS             complement(7743..9146)
                     /gene="yeeO"
                     /locus_tag="IT904_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014832583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAP3192693.1"
                     /translation="MWFHFLQLRSALNISSALLQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFY"
     gene            9307..9382
                     /locus_tag="IT904_002943"
     tRNA            9307..9382
                     /locus_tag="IT904_002943"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9340..9342,aa:Asn,seq:gtt)
     gene            complement(9420..10370)
                     /gene="cbl"
                     /locus_tag="IT904_002944"
     CDS             complement(9420..10370)
                     /gene="cbl"
                     /locus_tag="IT904_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAP3192694.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(10472..11389)
                     /gene="nac"
                     /locus_tag="IT904_002945"
     CDS             complement(10472..11389)
                     /gene="nac"
                     /locus_tag="IT904_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAP3192695.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMLRITTPSMSLSLSLNLLARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            11715..11790
                     /locus_tag="IT904_002946"
     tRNA            11715..11790
                     /locus_tag="IT904_002946"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11748..11750,aa:Asn,seq:gtt)
     gene            complement(11846..12781)
                     /gene="ldtA"
                     /locus_tag="IT904_002947"
     CDS             complement(11846..12781)
                     /gene="ldtA"
                     /locus_tag="IT904_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP3192696.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(12843..13922)
                     /gene="cobT"
                     /locus_tag="IT904_002948"
     CDS             complement(12843..13922)
                     /gene="cobT"
                     /locus_tag="IT904_002948"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAP3192697.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(13934..14677)
                     /gene="cobS"
                     /locus_tag="IT904_002949"
     CDS             complement(13934..14677)
                     /gene="cobS"
                     /locus_tag="IT904_002949"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAP3192698.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(14674..15219)
                     /gene="cobU"
                     /locus_tag="IT904_002950"
     CDS             complement(14674..15219)
                     /gene="cobU"
                     /locus_tag="IT904_002950"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAP3192699.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         4178 a         3830 c         3372 g         3980 t
ORIGIN      
        1 gtctatgccc ctacaggcgc tactctgacg gcaacgctaa cctctgcaaa tggcactcca
       61 gtggagggtc aggtcatcaa ctttagcgta acgccagaag gggcgacgtt aagtggcgga
      121 aaagtgagaa ctaactcttc aggtcaggct ccagtcgttt tgaccagcaa taaagtcggt
      181 acatatacgg tgactgcatc tttccataac ggcgtaacaa tacagacaca gacaaccgtg
      241 aaagtcactg gcaactcaag caccgcccat gttgctagct ttatcgctga tccatcgact
      301 atcgccgcca ccaacactga tttaagtacc ttaaaggcaa cggttgagga tggcagtggt
      361 aacctgatcg aaggtctcac tgtgtacttc gccttaaaaa gcggctctgc cacattaacg
      421 tcattaacag cggtgaccga tcaaaacgga atcgcgacaa caagcgtgaa aggagcgatg
      481 acaggtagcg tcacggtaag cgcagtcacg accgctggtg gaatgcaaac agtagatata
      541 acgctggtgg ctggcccggc agacacctcg cagtccgtcc ttaagagcaa tcggtcatca
      601 ctgaaagggg actataccga tagtgctgaa ttacgtcttg ttctgcacga tatatcaggc
      661 aatccgatca aagtttctga agggatggaa tttgtgcaat caggtactaa cgtgccctat
      721 ataaaaatta gcgcaattga ttacagtcta aatatcaacg gtgattacaa agccactgtt
      781 acaggcggcg gagagggtat cgcaacgctg atccctgtat tgaatggtgt tcatcaagct
      841 ggtctgagta ccacaataca attcactcga gcagaagaca aaataatgag cggtacagta
      901 tcagtcaatg gtactgacct accgacaact acattccctt cgcaggggtt caccggggcg
      961 tattatcagt tgaataatga caactttgcc ccaggaaaaa cggcggctga ttatgagttt
     1021 tcaagctctg cctcctgggt cgatgttgat gctaccggta aagtgacatt taaaaatgtc
     1081 ggcagcaatt gggaaaggat tacggcgacg ccaaaatcag gaggccctag ctatgtatac
     1141 gaaatccgtg tgaagagttg gtgggtgaac gccggcgatg ctttcatgat atacagcctt
     1201 gctgaaaatt tttgcagtag caatggctac acacttcccc gtgcagacca tttaaaccat
     1261 agtcgttccc gaggcatcgg gtcactgtac agtgaatggg gagatatggg gcattacacg
     1321 actgacgctg gttttcaatc aaatatgtat tggtcatcta gtcccgcaaa ctcaagcgaa
     1381 caatacgtag tttccctggc aacaggtgat caaagcgtat ttgaaaagct tgggtttgct
     1441 tatgcgacat gttataaaaa catctgattt tcctctataa tttcttaaag agtgctccat
     1501 tcggggcact ctttttttac attccctcta tagagcttgc tcttctctca cagaaaatga
     1561 ataaataaaa atgcgatacc gccagattcg cgttcggtgt gactatgatc attttcggtg
     1621 agtacagctt tgatccaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
     1681 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
     1741 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
     1801 caacgacgac tgactccgaa acatcggcca ttgcgggcca tcgcgttttg taatcagcat
     1861 aaattgcaac aacgggagta ttataggcac ttgccatatg taatattgag gtgtcaggag
     1921 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
     1981 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
     2041 gtccatgaac gatcactatt ggcatatctg tttcactttg aatttttgct attagatttt
     2101 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
     2161 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
     2221 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
     2281 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggaga
     2341 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
     2401 ataacatcgt tttcaggttc tttttacgca tcccctcaat gcaaagatcc ggcgtaccgt
     2461 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
     2521 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
     2581 aattaattgt cgacattaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
     2641 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatggatg acaacaaagt
     2701 ctgttttttc aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc
     2761 tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca
     2821 gtctatcagg ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct
     2881 ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
     2941 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
     3001 gtcgaggtag tctcataaga ttcgtttata gatcgacggc aatgtgagtt accttttcca
     3061 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
     3121 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc
     3181 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg
     3241 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct
     3301 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
     3361 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
     3421 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
     3481 gtgacactgc gtgccattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
     3541 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
     3601 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
     3661 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctatttag catcgtactg
     3721 gtactgggag cattgtgggt gcgcaatggc atggaagagt ccgcggaatt tgaacaacag
     3781 caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
     3841 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac gatgtacatc
     3901 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg cgaacttttc
     3961 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
     4021 cttgccgatc gttttggtcg ccgcagggtt tatatcacag gtgcgttaat cggaacgttg
     4081 agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc
     4141 tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt
     4201 accgaaatgt ttggtgccag ttatcgctat agtggcgctg gagtcggtta tcaggttgcc
     4261 agtgtggttg gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc
     4321 gggaactggc atagcgtcgc catttatttg ctggctggat gcctgatttc cgcaatgacc
     4381 gctttgttga tgaaagacag tcaacgcgct tgatagcctg gcgaagatca tccgatcttc
     4441 gccttacact tttgtttcac atttctgtga catactatcg gatgtgcggt aattgtatgg
     4501 aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag gcactggata
     4561 aactcgacgc gctgtatgag caatctgtag tcgcattacg caacgccatt ggcaactata
     4621 ttaccagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt gtctatccat
     4681 cactgaccgt aacctgggac ggtagcacaa ccaatccccc caaaacgcgc gcatttggtc
     4741 gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt
     4801 atcttaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc tctgtgcaac
     4861 cctcgcagca tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc
     4921 gctcaatgag cgctgggtta actcgctact tcccgacaac agaactggcg caaattggcg
     4981 atgaaactgc agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg
     5041 cgcgccgcgt cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagttgaac
     5101 attttcagcc gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt
     5161 ggggatgcag ccagatcctc gatcctgata gtccttacat tgccctttct tgtgctggcg
     5221 ggaactggat caccgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga
     5281 aaaaacatca gatgccagca tggcatttaa ttaccgccga tggtcagggt attactctgg
     5341 tgaatattgg cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg gcagtgctac
     5401 gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagt caggccattg
     5461 gcgattatgt acttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc
     5521 cgcccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat gacgccacca
     5581 agctggtgag cggcaggccc ggtgaggaag tcaaacagcg gctacgtact ggtactgtgg
     5641 taaccacaga tgacaggaac tgggaattac gttactcagc ttctgcactt cgttttaact
     5701 taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt
     5761 tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat ggcgagatta
     5821 aacttcccgg ccaggctaac cgtttttatg aaggcgctat ttccgaacat ctgcaaattg
     5881 gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattcg cgtaaattac
     5941 gaacctttaa tgagccgccg ttccgataat aagttctgaa ccttctcatc agaaatagat
     6001 gtgaaggagt aagtaagaca cctggcaaat agcctgcaat aatcgtgggc tgtttgcttc
     6061 cttgggcgga tacgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat
     6121 gctttcaaaa cacaattata aaaaatcccg ccaacaatat aagtttttat aaaattaaat
     6181 ataagattat ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat
     6241 ataatatgcc gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg
     6301 agatcattcc agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg
     6361 gtgcaacgtc taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gctaaacaag
     6421 gtgaacccga tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg
     6481 cacaagttcc aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg
     6541 aaacgttcgt gcagggacgt tatgaaggct ttggtcctaa tggctcaatg attatcgccg
     6601 agacattgac ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa
     6661 aaggcggcaa tatcggagcg gcaggttctg tcagctatat gtttgacaat actggtgtga
     6721 ttgtatttaa aggatcagac cctgaccata tttttgaaat tttacttgaa gctgaagttg
     6781 atgttcgtga tgtgactgaa gaagaaggta acattgttat ttataccgaa cctactgacc
     6841 ttcataaagg aatcgcggct ctaaaagcag ctggaatcac tgagttctca acaacagaat
     6901 tagaaatgat tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag
     6961 ggcttgttga tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa
     7021 atctctaatt atatgttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa
     7081 tccttataaa aaatataatg ttcaactcgt catattgctt ataaaaaatc ctcgttccca
     7141 gagaaataat atttattata attccagttc ttctttttct gattacagaa ggcaaagtga
     7201 caattacgca tagtttcccg ataaagacgc gacagctaca tcccgcataa ggcatttttc
     7261 tctttatcct tgtacggtac ttcatggaac agagtttttg accttgcgaa tcgtgatgtc
     7321 tgttggggag gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca
     7381 gaaatcaaaa aacccggtca cttttttgca aggtaaccgg taaaaataat ttttgttttt
     7441 taactgtttt gagactcata gagatgtctc aaaactaaaa tttggctcct ctgactggac
     7501 tcgaaccagt gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat
     7561 cgtgataacg aggcgaatat tagcgatgcc cacccacaat gtcaaagcct gttttttaaa
     7621 tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt attccgacac aactggcttt
     7681 ttttcacact tttgcggttc ggatctaggg tatttccaca gccaacgtcc agtaaccatt
     7741 cgtcagtaaa acagcacggc ccgcacagcc cagtcggcaa acattcccat ccagacacca
     7801 accacacccc agccaagcat gatgcccagc acataaccga cgacaacccg gcaaccccac
     7861 atgctcaaca tagaaaccca catggcgtaa cgggcatcgc gagcaccttt aaatccagcg
     7921 ggtagcaccc atgaggcgga ccaaataggc ataaataaag catttagcca aatcagaatc
     7981 acaacgacat gtttaacctg tggatcctgg gtgtaaaacg atgccataac cccggcaaag
     8041 ggagccgtta gccaggcgat ggccgttaat ccaagagtgg aaagccagaa cacatgccgc
     8101 aactgaatct ctgcttgcgc tatctgccct acccccaacc ttcggcctgt aatgatcgta
     8161 gaagcagagc cgagcgcact tccgggtaag ttgataagag ccgcaattga aaacgcgata
     8221 aaatttccgg caataacact ggtccccatc ccggcaacga acatttgggt taataaccga
     8281 ccactggtaa ataacactga ttcgacactc gcgggaatac caatccccat gacttcccag
     8341 ataatgctaa aattcagcgg tttaaaatag ctctttaacg aaatccttag cgcaggatta
     8401 aaaccaatcg ccagcaccca caaaattgca actgcgccaa tataacgaga aatggttaaa
     8461 cccagccctg ccccgacaaa tcccagtccc ggccaggaga aaaggccgta aatcaatatg
     8521 ccgctaataa taatattaag aatattcagg ctaccgttaa tcagtagcgg tattttcgta
     8581 ttccctgcac cacgaagtgc cccgctacca atcaaagtga tggcaactgc tggataactg
     8641 agtaccgtca gctccagata agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc
     8701 gcgacgaaat caataatttg ttcgccaaaa tgatgaataa gcgttgccaa cagtacggca
     8761 aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg tcgatcccgc
     8821 ttaccgagac taaatgccac aacgacagta gtaccaagat cgatagcagc aaaaaaagcc
     8881 ataatgacca tattgaagct gtccgccaat cccacgccgg ccatcgcatc ttttcccagc
     8941 cagctgacca gaaaagtgct caggacaccc atcaacagga cacaggcatt ctccatgaag
     9001 ataggaacag caagcggggt tatctcgcgc cagaacaaca ctttgtagct cttgcgttta
     9061 gcgtgccagc gagtgccgtg aacaacctgg agtaaagcag aggagatatt caaagccgac
     9121 cttaattgca gaaagtgaaa ccacatttca aataatgagg gagaatcagc aaagctgcaa
     9181 agattttcgc caacaaattg tctgcaaatg caacaaactg ttgatagaaa cggcaaacag
     9241 ttgggaaatt taaaaatcgg gtttgacaaa agatttttcg ccgttaagat gtgcctcaac
     9301 agcgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt
     9361 tcgagtccag tcagaggagc caaatttctg aaaagcccgc ttttatagcg ggctttttgc
     9421 tatatctgat aatcaatttc ctcttcactg ttctccatca cctggcgctt gatatcctca
     9481 actgacagtc ctgcattaca aagttccaga aagcgccaga catagttacg ttgaagttgc
     9541 cctcgcttca gtcccaacca tacagtattg gcatcgaaaa gatgccgcgt atccagacgg
     9601 attaaattct cttcctcttg ttcgccgctg gattgttcgg caactaatcc aatccccagc
     9661 ccaagagcaa cataggtttt aatgacatca gaatcctgcg cacttaatac aatatctgcc
     9721 agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc gccccgtaat cccctgtcgg
     9781 taagtgatta acggccactt cgctattgat tccagcgtca acggtgtaat ttgcgtcaag
     9841 ggatgatcga gtggaacaag caaactatgg tgccaacgaa accacgggaa ggcgacgagc
     9901 tgcgggtcat tactcaaacg ctcgctggcg ataccaatat cagcttcgcc attttgcaac
     9961 aacgtcgcaa tttcctgtgg cgtaccctgg attagctcca gccgaacctc cgggaaaagt
    10021 tcgcgaaaag ctttaatgac ctcaggcaag ctataacgtg cctgagtatg cgtcgttgca
    10081 atagtgagaa cgccagacgt atcgttggta aacaggtctg caagccgacg aacattactg
    10141 gcttcattca gaatacgttc tgcaatgacc agtaatgctt tgcccggttc agtcatgccc
    10201 agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc cagttcccga
    10261 atatgacggc tgacgccgga ctgcgaggta aaaagcatat tcgcaacctc tgtcaggttg
    10321 taatcctgac gtgcagcctc gcggattatc tttagttgtt ggaaattcac ggtaaactcc
    10381 gggcagctca gatttcccgt tattgttaaa gtctaatgcc cggcataaca aataataaaa
    10441 acccgcatct tattccatcc cgatataacg cttagctcac caattgccac tgcctttttt
    10501 ccatcactgg agaactaatc actgacatta acaactcttt cactgcctgt gcctgtggcg
    10561 ataagttcgc tctggcgagt aaatttaatg acaaagagag actcatggaa ggcgtggtaa
    10621 tgcgtaacat ccacccattt actgcgccac ataacgaacg cgcggccgat tcgggtaata
    10681 ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt ggcaatagac tcaatttcac
    10741 caatgacttt tgccgtgagc cgccgtaggg aaaaagcctc atcaacacga agtctaacag
    10801 cactgtaatc actggggaga aagaggttca tttgcgcaat agcattaaca tcaacgcttt
    10861 gccccgggca atcttgagtt cctaccagga aaagatcttc tttcagcaaa gcctgactgg
    10921 ataccccagc cacaggggaa tgctcataaa tcaccgccat atcgagttgg tgatttatca
    10981 gtttttcgtt aagcactgca ccgctatttt catgaagata gataacgatc tccgggaatt
    11041 cagcgcgaac cgcttgtaat aagggcatgg tgatagatga cgcagcggtt cctggcgcaa
    11101 agccaatcga gacttgcccc gataatgcct gaccaacgtt atgcaccgcc agttgggcct
    11161 gctcacactg acgtaaaatg gcccgcgcat gggtatagag aatttttccg gcgtctgttg
    11221 gcgtaacgcc ccgttttgtg cgaatcaaaa gttgttgatt taactcacct tccagtgtgg
    11281 caacctgctg gctgagcgct ggttgtgcga tatgcaatac ttcagcagcc tgagtcaggc
    11341 taccaatatc tacaattttt acgaagtatt tcaggcgtct gaagttcatg ttgcctccgg
    11401 tttttgagaa tcggcccaag tgccgccatt acttacaacc agactgcaag atgcttgcca
    11461 gttttatttt ggcgttgatg tacaagctaa ccaactgtca aataagagat tatgatagat
    11521 tcgtcattgg ctcttttcac cagcagtcgc gttcccctgc cctataaaag gagggtatgc
    11581 accacgatgg ttcattacca ataagattga aatctcatca ctttgttgaa attggcagca
    11641 aacaaacaaa aaaatgcatt tcaccctttg acatcaccat gcactgccat taatatgcgc
    11701 cccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
    11761 tcactggttc gagtccagtc agaggagcca aattcaaaaa agcctgcttt ctagcaggct
    11821 ttttgctttc taattaccaa cgctcttaaa acatctgtct tgaaccagaa ctaatttgca
    11881 caggcattcc tgatcgacgt tgcaacgcag catttgcgcg attcacatca acttcttgcc
    11941 cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg cacttttcgg tcagactcat
    12001 attctgcacg attgcgcgac agcggctcat gaacttccag ccagttcgag ccatctggtt
    12061 cagtggtgta ttttactggc tggtcgataa tttgcacacg cgttccaaca ggaacattat
    12121 caaacagata tttgatatcg tcattgcgca gacgaataca gccctgactt acccgaagtc
    12181 caataccaaa attggcattg gtaccgtgga tggcatacaa cctgccaata taaatcgcgt
    12241 acagccccat ggggttatcg ggccccgcag gaacaaatgc gggcaaactc tcccctcgtt
    12301 tcgcatattc gcgccgggtg ttcggcgttg gcgtccaggt tggtgcttct tgtttacgtt
    12361 caacggtagt cacccagtta cgcggggttt ctcgcccagc ctggccgata ccaataggaa
    12421 agacttccac tgtattactg tctggcggat agtaataaag acgcatttca gcgacgttaa
    12481 ccacaatccc tttacgaaca gtggcgggca aaatcagttg ctgcggaatg gtgagttgtg
    12541 agccaggctt cggcaaaaaa acatcagcgc ccggattcgc ttccagcata ttacttaacc
    12601 cttgcccgta ttgtgcggca aaagtctcca gcggctgagt attgtgatca ggaacagtta
    12661 cagtaagcga ctgccccacc aaacggctac cctctggagg taacggataa gttaccgcca
    12721 ggctattttg gctggcaaaa agcagagcaa atgagcaaag aatatttaca cgacgcatca
    12781 tgtccctttc ctaagtcgcg aatactatcc gataagtata gcttttatca gacttttcgt
    12841 ttttagctgt tcaaatcaga agtcgtattc cccggtagaa caatattact ggcagcaagt
    12901 tcgcccatgt tgttgtatat cgcacaagca gcttcgatga tggacatcgc cagagctgca
    12961 ccactcccct cacctaaacg catatccata ttgagataag gctccagccc caaatgcgag
    13021 agcgctatac gcgcgccttt ttctgccgac aagtgagaag gaatgagata cggtttgatt
    13081 gcaggagaca tctggcaggc tgcgagcgca gcagcataag aaagaaatcc atccagcaac
    13141 acgggtaaac cgcaggaagc agcacctaac atcactccag ctattccgac caaatcaaat
    13201 ccacccactt ttgccaggac atcaacacca tcctgaggat ttggttgatt caacgtaatc
    13261 gcccgacgca caacatcaat tttattagcc agtttatctg tcggcaggtt tgcgccaatc
    13321 ccaaccactt cttcaggatc ccggccagtg attgtgctga ctattgccgc tgccggtgtc
    13381 gtgtttgcca tccccagttc acctacacca aacagcgtga caccattttt tgccagctcc
    13441 cgcgtataac atatgacgtc caaaagcaac ttttcagcct gacggcgact cattgccgga
    13501 gctgaagcaa tattgccgct acctcgtgcg acacgcatgt tgataagccc ggggataggc
    13561 tcagcagtat caataccaac atcaactacg tggacgttag cgcccgcttg tgctgccagc
    13621 acacacacgc cggttgttcc acgggtcata ttttcagcct gtatggctgt cacttctttt
    13681 ggagaaatag cgaccccttc ctcccagacg ccgtgatcgg cacacataac cagtaccgct
    13741 tttttgccca catgcggtat gccattcaac cccggcattc ctgccagttg tatggcaagc
    13801 gcctccagcc ttcccaggct accaacaggt ttgagtaacc cgtcaatatg ccgttgtgca
    13861 cgcgacatag cggcaggatc gatagcaggg atcgtattca gtaaatcggc aagtgtttgc
    13921 atgtcacgtc tctttatagc agtgccagca ggaagaccag ttcaccaagc tctatggctg
    13981 ctcccagcgt atcgcccgtt tgcccgccca gcgtacgttt gagaagctga ccgaggatga
    14041 aaatcgccac catcgtcacc accatagcag ccacaccatg catacccggc aataatacgg
    14101 cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg tcgcccatca attttgccaa
    14161 tgaacacatt gccaagccct tcttcccgcg cataacgatg acgatacatc aataatgcgg
    14221 cagtcccgcg gctgaccgca catgctgccg ccagcgatgc aaggatcggt tcgccacgca
    14281 gagccaactc gcttaacacc agaatctttg ccagtaccac aaaaatcaat gccagaccgc
    14341 cgtgggtgcc taaacggcta tcacgcatga tttccaacat gcgatcgcgg ctacgtgcag
    14401 aaaatacgcc atcacaggta tcagcgaggc cgtccagatg gaatcccccg gtcatcagcg
    14461 ccagcacaag tacgctaaac agtgccgcca gtggtgcgcc acaccatgcc tgcagcacca
    14521 tgaagaccag cccgctaatc gcgccaagca ataatccaat caaaggaaaa gtaataatac
    14581 cgcgagaata atgctcgaaa tccagtccct gggaccagcg acgcggtaca ggcagacgcg
    14641 taataaatga gagcatcgcc caaaataatt tgctcatttg atttttactc caatacccga
    14701 aaccaccagc catacttcat ttgccgcagc ggccaactgc tgatttaccc gcccggcaat
    14761 atcacgaaaa tgtcgtgcca gacgactctc cggcacaatc cccattccca cttcgttagt
    14821 cactaataca acctttgcgg ggcaacgttg gcaggcagca atcaacgact gaatctcagc
    14881 attaatcgcc tgttccatcg cctgataatc ccattcatca gggtctttat cgccgccata
    14941 atcaaacaac agattagtca ccattgtggt aacgcattcc agcaacacag cctcattcgg
    15001 gttaatgtct gcatgaatta attcatcaag atgttgccag cgctccaccg tgcgccagtg
    15061 ctccgggcgg ctttgccgat gatgttctat ccgtgcagcc atctcatcat caaggatttg
    15121 cgaggtagcg atatacagaa cctgtgaaga gtccccaata agcacctctg cgtggcgact
    15181 cttcccgctc cgtgcgcctc ccgtcaccaa aatcatcata ccgactcctg attcaaatgt
    15241 atgatggtat tcattgaaca gagcaatgga tcccccaccg ttgctgaccg caaaacattt
    15301 acgcgattaa tactgcgcgt aatataatta aaaatccagc attctcaatc cattacgaag
//
LOCUS       DACAPB010000107        15302 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.107, whole genome shotgun sequence.
ACCESSION   DACAPB010000107 DACAPB010000000
VERSION     DACAPB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.107"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(55..996)
                     /gene="fabY"
                     /locus_tag="IT904_002951"
                     /gene_synonym="yiiD"
     CDS             complement(55..996)
                     /gene="fabY"
                     /locus_tag="IT904_002951"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAP3192700.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDSVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1041..1478)
                     /gene="dtd"
                     /locus_tag="IT904_002952"
     CDS             complement(1041..1478)
                     /gene="dtd"
                     /locus_tag="IT904_002952"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP3192701.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1475..2347)
                     /locus_tag="IT904_002953"
     CDS             complement(1475..2347)
                     /locus_tag="IT904_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAP3192702.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2341..2940)
                     /gene="yihX"
                     /locus_tag="IT904_002954"
     CDS             complement(2341..2940)
                     /gene="yihX"
                     /locus_tag="IT904_002954"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAP3192703.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3081..3968)
                     /locus_tag="IT904_002955"
     CDS             complement(3081..3968)
                     /locus_tag="IT904_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP3192704.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(3958..5307)
                     /locus_tag="IT904_002956"
     CDS             complement(3958..5307)
                     /locus_tag="IT904_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP3192705.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPKLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(5406..6839)
                     /locus_tag="IT904_002957"
     CDS             complement(5406..6839)
                     /locus_tag="IT904_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192706.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(6909..7919)
                     /locus_tag="IT904_002958"
     CDS             complement(6909..7919)
                     /locus_tag="IT904_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP3192707.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(7952..8839)
                     /locus_tag="IT904_002959"
     CDS             complement(7952..8839)
                     /locus_tag="IT904_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP3192708.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAEQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(8864..9742)
                     /gene="yihU"
                     /locus_tag="IT904_002960"
     CDS             complement(8864..9742)
                     /gene="yihU"
                     /locus_tag="IT904_002960"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAP3192709.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGLGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            9915..10811
                     /locus_tag="IT904_002961"
     CDS             9915..10811
                     /locus_tag="IT904_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP3192710.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            10851..11639
                     /locus_tag="IT904_002962"
     CDS             10851..11639
                     /locus_tag="IT904_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP3192711.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(11750..13015)
                     /locus_tag="IT904_002963"
     CDS             complement(11750..13015)
                     /locus_tag="IT904_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192712.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(13117..14097)
                     /locus_tag="IT904_002964"
     CDS             complement(13117..14097)
                     /locus_tag="IT904_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAP3192713.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(14105..14815)
                     /locus_tag="IT904_002965"
     CDS             complement(14105..14815)
                     /locus_tag="IT904_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAP3192714.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(15031..>15302)
                     /locus_tag="IT904_002966"
     CDS             complement(15031..>15302)
                     /locus_tag="IT904_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192715.1"
                     /translation="YEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIR
                     MTLEQALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4101 a         3961 c         3557 g         3683 t
ORIGIN      
        1 aggactaata taaatgacct gaatgagaga ggcagcaccg cccctctcca tcagctactc
       61 ttcttcgttc ccgccctctt catacgggcc aaatggcttc gcgggcagaa cgatatacgt
      121 gccttcaaac actgcacccg gcgtctcgtc gccaaagatt tcgacctgca tctgcacccg
      181 tgcttttcgt ccgcgcgcca gacggtcgag atcgccgctt aaggcaccga ggtcggctac
      241 cgcatgaggt ttaccgctaa tcggcttgct gtagcggata tgcgcatccg caagaataat
      301 cgttccgccg aggtggcgtt cacgcagcat cagccagata agtccccaac cggtgagcgt
      361 cgccagtgag aataaactcc cggcaaacag cgtatggtgc ggattctgat tgccggtttc
      421 tggcatggta gtgataaatt tttgcccggt atattgctga atgcgcacgc ccattttttc
      481 actaagcggg atatgttcgt accacgcctg ttgcagctgc gcgcaccagt cgccgcgatg
      541 cagaatgtca tccagagtgg cgacgggctt aatcatcaaa aaatggcgaa tcggcgtggt
      601 ggttggcgtg gtgatttctc cctgattaac aaaccccagc ttggcgaaaa actccaccga
      661 gtcttcacgg gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca ccgactccag
      721 ggtcatcgcc atcagcgtgc ctaacccttt gtcctgcacg tcgggatgaa cggccataaa
      781 gcgaatggac gcttcattgt cggcattaat atacagtcgg cctaccgcca ccagattacc
      841 ctgctcgtcg acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc gttccgaacc
      901 ttttggttga tgcaggggct tacgcaacat ttcccagcga aactgatagt aacgctctaa
      961 ttcttcttct gtttgtggaa cccgaaggtg atacatagcg atactctctc ttgtttcccg
     1021 tgacaaccct ggaagctggc tcatacctgc aaccagaatg tcacggggcc atcattgacc
     1081 agcgatacct gcatatccgc agcgaagcgt cctgtttgcg tgttcatctc ttgctgacgg
     1141 cagcgttcga cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc acctttggag
     1201 aaacttgggc gcatcccccg ttcggtatct gcggcgaggg taaactggga aaccaccagc
     1261 acactgccgc ccgcctgttg cacgttgaga ttcatcttgc cttcggcatc gctaaagatg
     1321 cggtagccga gcacacgctc gcacagacgg tttgctttct gttcgtcgtc atccttttcg
     1381 acacccaata acaccaaaag tcccgcgcca atttcgcccg tcacttctcc ctccacggtg
     1441 acgctggcac gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct tcttcttgtt
     1501 cagcagcttg ttttagtttg cggtattccc cgagagtgac agtaatttcc gcgccaagca
     1561 agacgataca ccatgtccag tagacccaga caaagagaat ggggatcacc gccagcacac
     1621 cgtaaatgag ctgatatgac gggaacatgg tgatataaag cgcgaaacct ttctttcctg
     1681 cttcgaacag gagtgcagca acaaacgcgc cgacaatcgc gtcgcggtta ggtacgcgga
     1741 tggtaggaac aatgctgtac agcaaccaga aggagatcca cgacaacagc agcggaaaaa
     1801 tacgcagcac gttgtcgatg acagtattga gatcgctcgc ccagcgcagg gagagcaaat
     1861 aggaactgat cgccagacta gcccctgcca gcagcggccc cagcgttaaa atcatccagt
     1921 acacggcaaa cgagtaaatt tttggtcgcg ctcgtttact gcgccagatg gtattcaacg
     1981 cgctatcgat ggagtacatc aacaataacg cggtgacgat cagcccgcac gcgccaacgg
     2041 cggtcatctt gttggaattg gcaacaaatt gttcgatata ccgctgaata acatcgccag
     2101 tagcaggcag aaagttggca aaaataaagt gacgcaactg aatgctgacg tcggaaaaca
     2161 tgggaaaagc ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat gagagcaacg
     2221 acacataggc aaggttacct gccagggttg tcatgttgtc ctcatcaata cgttgccaga
     2281 gtagttttag ccaggcccat agtggacggg tacggtgcct ggctttgtcc tgaatggttt
     2341 ttagcataac accttcgcga aatagtccgg gatggtggtt ttatctttca ccagaatact
     2401 ggtaatgcct agctgattag ctccttctat attatcggcg ttatcgtcga aaaagaccgt
     2461 atcgctgggt gaaaaacctt ccgcctgcaa aacatgctgg taaattcgag cttcaggttt
     2521 gcgcatcccc agatcttgcg acagatagat atggtcagca gcatcacgaa tttccgggta
     2581 ttcttccggc cagaaggtgg tatgcaggcg gttggtattg gaaagcacca ccacgcgatg
     2641 cccctgctca cgcagtttat gcatgatggc gatcacttcc gggcgcagcg caacaaacac
     2701 cgcctgccag ccgtgagaga actgctcgta gcttagcggt agagccatct catgacacag
     2761 cgcctctgcg aacgcttcgt cgctaatttc cccacgctca tgctgatgaa acgcctcccc
     2821 catatgaaaa ctcttcttaa gcgatgccag cggaatacgc gttaaatcgc tccaggctcc
     2881 cagcacacgg ttaaagtcga tatcgacaat cacattacct aaatcaaaga tatagagcat
     2941 ttttgcctcc tttcgcgcca caagaaatta actgtagcgg gaaaggatgg gtttggctat
     3001 gtggcaataa gggtaatctt gtgatgctga ttgcgaaaaa gcagagtaat ttcactggct
     3061 ttttcgcaat agatgaatgg ttaagattca ggtgtaaatt gccaacgagt ttcgatgacc
     3121 agcggcctat ctggtgtcac caattccggc gttagattta ttgcgttagg tgcctgagtc
     3181 agcgggttaa tacaggtggc atcgggctgt ttatcaaata ccaccagact gtttgcagaa
     3241 gagatcatgg tcatcgcgag tttaccaggc cacagtaact taaccttcac cccgtcataa
     3301 aaaccaaaac agtcatccca gggaccggct tgaggagaga tacgatttcc agttggcagt
     3361 tcatcactac ccgcttcttc ttgccagtcc gcatcaaaaa gcacctgcaa cgattctgta
     3421 ttttgcggag tcagcttttt cgcaaaccac ggatgccagc ctgcggaggc cgggaatgta
     3481 tcggcatatg aatggacttc cagttgcaaa accagcgcat cgttctccag caaaaaagtc
     3541 tgaatcactt ccccttgcca gggccacggt gaagccaggg gcatacgtaa agtcagggaa
     3601 tctgctgttt tgtcgatgat ttcccacgtt gagtaacaag ccataccatg caacgcatgt
     3661 ggtggtttat tggcgggtaa agaataacat tgcccaccag cattcagcgt cgcattcccc
     3721 agtcgccctg cccacggcac cattgggaaa cagccatact gaaaagctct gcgctgcggc
     3781 tgccattggc gtaatacttc agaaccaaat gcctttaacg aggttatcct ggcaccatca
     3841 tgcggataaa tctgcaggtg aatattgcct ttttccagcg tcactaactc tcctacaggc
     3901 ccaggccatg gcgcgtcaaa cgcctgttgt agcgccaggt aatgctcgtt ttttcgttca
     3961 gttttcatga gattgttccc tgatgtagcg gtaagaagcg gtttcttgtt catcggtcgg
     4021 ctcaatcagc cagacgcaaa caaaggaaag aactgagaca gcaaataaaa acagagcgac
     4081 ataccacggt tttcctcccc ctagcgccag caaacttgag gcgataaggg ggcttagtcc
     4141 gccaagaatc gcggaaaatt gataagcaaa agaaaggccg gtatatcgta ctttggtacc
     4201 gaacatgcgg gtgaaaagtt ttggctgcac ggcaaacatc atcgtcggac ctaagttgta
     4261 gccgaggatc atactgcacc agatgatgag cgtacttttg ctatccagta ataagaagaa
     4321 aggaaacgcg aacaggacac aaaatcctgc tcccgacaaa tacagacttt tttgacctat
     4381 acgatctgat aaccatccaa ccaatggaca actgaaaata ccgatcgccg aggcaatcaa
     4441 catacccgtc aggggaatat ctcgcgacaa tgctaattgg ctggaaatat agacaatacc
     4501 aaaggcgtta ataatattag aggagacact ttccgccagc ctcgcaccca gtgccaataa
     4561 aatatttcgt ggatgttttt tgaataattc aatcaacgga ggaacggact tttctttatt
     4621 attcgtcgtt ttttgctttt gaaaatccag cgtttcttcg gtatgcaggc ggataaacag
     4681 cccaacgatc aacatcaccg cagaaagcca aaacggaatg cgccatcccc aggagagaaa
     4741 ctgctccgaa gtaagaaaat ggttacagag agcaaaaata ccggttgcca gcataatacc
     4801 cacagacgca gccgtttgcg ggagtgaacc taaaaatccg cgtcgggatt cgggcgctga
     4861 ttctatggtc atcaatgccg ctccgccata ctcgccacca agaccaaagc cctgaattaa
     4921 acgcagcgcc attaatatga gcggtgccca gatcccaatt tcctgatacg tcggaataaa
     4981 accaatcaaa aatgtggagc aaccgacaat agccagtgtc cagataagtg ttatttttcg
     5041 cccaatttta tcgccaaaat ggccaaagac gataccacct aaaggacggg tcaaaaaacc
     5101 aacgccaaag gtagcaaagg cggctataag acctataaaa ggatcgctgt ctggataaaa
     5161 cagcggtgca aataccagtc ctgctgccgt gccaaaaata aagaagtcat accattccat
     5221 taacgcgcct gcgaaactgc ccataacgac acgtctcatg gctttaccgg aaagcccttc
     5281 acgcggtgcg ggtaactttt cagacataat tttcctctcc tggtaggcga gaaatctggg
     5341 atacgaacca ctaaacgtgg gcagtttgaa ttaatgcgca tcggtttaag ccgatgcgca
     5401 ttaatttatt tcttcaattc agccagaacc tctgcatcct cagcatcaac atttgcaccc
     5461 agtggttccc aattaatcaa tacgcaggtc aggaacccca agaatggcac gattgccaac
     5521 cagtaaaacg cttcagtttt caacgccccc cacagcatcg gccagaaaat gagtcctgca
     5581 attgcccctg tgcgcataat agcgcgggca aatccgaccc cagtagggcg aatagcggga
     5641 ggataagaga gtgtggcaat ggtcatgccc agcccaccag gacccgctga atggaagaaa
     5701 ataattgatc ccaataaccc taaagacagc catggattag tcgttgccag tgcgcctaac
     5761 gacagcaatg ctaaggtcac aacagcaaat ccatacatcg actgtcggcg agtacccagt
     5821 cgttggagaa taaacgagcc gatcatcccg cctgtcaccc cacaaagggc attcacgacg
     5881 gcagaacccg tcaacgcacc agtaagcccg ccacttatta ttcccgccaa cgtaagagga
     5941 agataaacac ctacggcgtt atattgccac gcctgcatgg ttgccacgac acagccaaga
     6001 atggtgcgtt tgcggtaacg atcattaaat aagatgccat atccaccact gtatttattc
     6061 tctgcttttt ccgagaattt cgcttctgtt gtactcgatg ccgcaacgcg agcctgaaca
     6121 ccataacgtt ttcccagaat gtcgcacgct tcctggtaac gcccgacgcg tgcggcccac
     6181 attgcggatt caccaataaa gaagtaacgc aaaatcatga agatgactgc aaaaatagct
     6241 ccgatgaata ttccgtaacg ccacaattgt tcttcagcga tacccgacag aagcagcggc
     6301 aagagcacca aataaacaac agttgtcgaa acataccagg ccatttgcca gaggttgacc
     6361 gacgttccgg ttttcttcga cgttgaacca cgaagctctg ccacggcatt ggttgccaaa
     6421 gggaagtcga tgcctaaacc aaagcccatg atgacgcggc acaccagcac ccaccagata
     6481 ctgggcgcaa tagctaccgc agcagccccg atggtgcaca gccccatgcc aataataaat
     6541 gctcgctttt gcccaaagcg gtttattact cggttcgcta ataaaccgcc aattaacgcg
     6601 gcgattaata ctgacgcatt aacggttgcg gcaaggcctg gagaaatgcc aaattgagcg
     6661 gcaatgtatt tattaccaaa ccccaccatc gccgcctggt aggcatcaat caatatgccg
     6721 cccaaagcaa taaaaataac gccgatactg gtgttgattg cagagttctt attaacaata
     6781 tcaattacgt cctgtggccg actaattgaa taagttgcag gactggtgat ttgactcatc
     6841 gcagtgtcct ttttaattgt tgtagggtgg cgctgacgtg acgcagcgcc agacagagat
     6901 ttagaaaatc attccggcgc aaattgcagc atgaccttta tggagtcatt ttgctgcgcg
     6961 cgttcgaatg ccgccgctgc atcctcaatg gcaaataaat cggtaaccag ttcactggta
     7021 tcaaaccgcc catccgccag ccattcgata acagcatcga aatcttcagg gatgtacatg
     7081 ccagagttga gcaagtcacg ctcgaaacgt tgcatcatcg gtaatggtat ttcacgcggc
     7141 ccacttggta cgcccatgca cacgacggtg cctccggcat atacacgggt acatgcatcg
     7201 ttaagcgtgg cctgcgctgc aacgacatca atcgcccccg taaatcgcac atctgcggcc
     7261 agctcacctg gtgcccaaac ttctgctgcg cccagcttta acgccagcgc gcgtttggca
     7321 gcgtcaggtt caatcacggt aatcttacct gcccccataa tgcgcagtgc ctgcacgatg
     7381 gataagccta tcgttccggc accaatcacc agtacgcttt ccagagaagc tttcggcatt
     7441 cggttaacgc agtgacgggc acaagccgcc ggttcggcaa aggccaacac ttttagcggt
     7501 aaagaggctg gtgcgacatg gcaattacgc gcaggaacaa tgtgttgtga gcgagcaaag
     7561 cccggtgcgc gaaaaccagc aacctgcggt ggttcacaaa gattaaaacg tcctgctttg
     7621 caggctcggc attccatgca agccatgatg ggatcgacca caacatgatc gcccggctgt
     7681 acatttttaa cgtcgctgcc aacttccgta acgcgcgctg caatttcatg accggggacg
     7741 acaggtggtt tagcaaacgg atgaccgcct ttcagaacat gcagatccga accgcatatc
     7801 ccatagaaca caggcgcaat ccgcacttcg tgcggtttaa gttcctggtg tggtacttca
     7861 acatattgag tggtgacttt cccaatatct gaaaataaaa cctgggtaat tttatccatt
     7921 gttattctcc agaatcgata tccatagggg attagcgttg cgcgatacct tcatcgacaa
     7981 tttctgccaa ccgttgtaaa cgaggaacag agacatctcg cagcatagtt tgtggatctt
     8041 gtgcaccaac aaccgacgcc caaaccgctc gtccagccag gaagcctgac gcaccacctt
     8101 tcatggcaat acttactgca cggccaaaaa tatcggcatc aacgccagaa gaaagaatta
     8161 cccacggcat cttcatctga tcgttaagtt gctgacaggc ggcaagtaag gttttttcat
     8221 cgcctttgcc ccctaatggc atctcggctt tgtaaagatc agcctctgtt ccaccaagct
     8281 ctgcggcggc ggcaacaatg gcgctttcac gatcaaagtc ccaaccgcga cgaggtggac
     8341 gcaccacagg ttcaataatg ctaaccagcc cggcactacg gcagcgacga acaaatttat
     8401 caaccattgc cagacgttcc tctgcgggtt catcttcacg ccagagtaca agtaatttca
     8461 ttgccttagc ccccatttca cgggctttat gcgggtcgac ggacatatct atttcaacgc
     8521 tatcaaccgg gatgccattg cctgggataa aaagatcggc agcgacaatc aggccgcaag
     8581 aattagcaac agcgccctgc tcgactattt gctcaagaca aaattgttta tccgccagaa
     8641 cggcagaagc ataaggagaa aggattcggg tcgcggcaac tttaaaatcg gtgagtacac
     8701 tgtcggcgat gggttttggc tgctctgctg ccgcaaacat caagcgcatg gcctcacgct
     8761 gatcgacagc taacatggca aatccaccac ctgggcggga gatatctttc agcgtaaatg
     8821 gggtgaagtg ggatgacata gaaactcctg ttaatagata aaattaaaga ccagcctgct
     8881 ggcgaacctg atttagcagg gcactccagt cctgccgccc tttgccgtct tgacttgctg
     8941 cagcgtaata actttctgct gcaatcccca tattgagcgg tgcccccgtc tcccgagcga
     9001 cgtcaactgc aatacgcaga tctttcaacg ccagatccac catgaaggcg ggtgaaagat
     9061 cgcctttcag cactttgcaa ggccaggtgg tcgtaaaatg tcctttccct gctgccgtac
     9121 cactcatcac cttaattgcg acatcaagat ttaatcccag tgactcacat aacacagcgg
     9181 cctcggagga cagcgcatta agcgcaatgc tcatatagtt gttaattagc ttcacgcgga
     9241 tccccattcc taggccgcca gcctcgacca gctcattacc catgcacatg agaatcgggc
     9301 gtgcacgtgc aatctgctca tcagtgccac ccgctaaaat cagcaatgtg ccatcaatcg
     9361 cattcacgga cgttctgccc acgggggcgt ccatcatact gatgcctttt tcttgtaact
     9421 cccgtattaa tgcatcggtc tgtaagggat gaatagtcga catatcaatc aataacgcgt
     9481 cagatgaaac agcctcacag acgccttttt cccccaacag aacctgtcga acaatgtcac
     9541 cattgggaag catagtgatg ataaattcag cgcctgctgc cgcttccgct ggggtttgtg
     9601 ccgcagtggc cccctgcgta actagcgcat cgacggcctg tgcattgaca tcaaatacct
     9661 gcaatgaatg ccctttttgc aatagattgt tagccattgg gctacccatc tgccccagcc
     9721 cgagaaatgc aatggttgcc atgaatactc tcctgtctga cattgacaaa ataatgacaa
     9781 ttgaaaattc gttttcgtct gtttttgagc atatttgtaa tttatgttca aaaacacgac
     9841 acgcatcact aatttgatca tttatgtcat ttaaattgac cgaagacgac aaaattaaac
     9901 aggacaagac aaatatgacc aaaattgcat gcgtgggtat cacagttcag gacaggattt
     9961 attctttgct cactctgccg gatggcggcg gaaaatatca ggcaaatcat tatcttgaga
    10021 ttggtggcgg cccggcagca acagccgctg ttgcaatcgc aaagcttggc gtggaggttg
    10081 atttcatcgg tcgcgtcggc gatgacagtt gtggaaatac cttacttgca gaacttgagg
    10141 gttggggcgt caataccgca ttttgccgcc gatatcctaa cgcccgttca tcgcaatcag
    10201 cgatcctcgt tgatcagcat ggcgaacgga ttatcgttaa ttatccaagt cccgatctcg
    10261 gtactgatgc cgaatggtta gaagcgatcg atttctctcg ttatgatctg attctggccg
    10321 atgttcgctg gcatagcggt actgaaaaag cattctcgct agcgcgtctt gcgggtgtga
    10381 caacgctttt agacgccgat atgacaccgc aggatatttc tccacttgtc gcgcttgccg
    10441 atcacgccgt gttctcaaca ccagggctga aacgcatgac tggcctgcag tcacctgaag
    10501 aaggtttgtt ccaggcaacg acacaaacag caggtaaagt gtatgttacg ctgggttctg
    10561 aaggatcgtt gtggattgaa gatggtcatt tgtgtcaaca ggaggcattt tctgtcaatg
    10621 tggttgatac cactggagca ggcgatgttt ttcacggcgc actcgcggta gcgttggcag
    10681 aaaaaatgcc aacaaaagag gctattcgtt ttgccagtgc ggttgccgcg atgaaatgta
    10741 cccagcctgg cggtcgggcc ggcattccta atcgtgagca aaccgaatca tttttgtcac
    10801 tttatgcgta aaatggtaaa catctaccga ggaggggcaa gggagtattc atgagtatca
    10861 tcgaagtgac aggtaatcca cgccatgacc aactcgttca tctaatcgca gaacgcggtt
    10921 atatgaatat tgaagaactg gcacaactgc tggatgtttc aacgcaaacc gtgcgccggg
    10981 atatccgaaa actcagtgag cagggcttga tcacccgtca tcatggaggg gccgggcgcg
    11041 tctccagcgt catgaatacc gcttttgagc aacgggaact ttcgctcacc gccgagaaac
    11101 gggcgatcgc tgaggcagtc gccgattacc ttcccgaacg ctgtaccgtc tttatcacca
    11161 tcggtacaac cgtagaagcc gttgccaggg cattactcaa ccggcgtgat ttacgcatta
    11221 tcactaacag cctacgtgtg gcacagattc tttataagaa tcaggatatt gaagtgatgg
    11281 tgccgggagg cactttacgc gctcataacg gcgggattat cggcccagga gccgtggatt
    11341 ttattgaagg cttccgcgca gattatttaa tcaccagtat aggtgccatt gaacatgacg
    11401 gcaccctact ggaatttgat gtaaatgaag cgttagtcgc gagaacgatg attaaacatg
    11461 cgcggaatac attgttagtg gccgatcata cgaagtttgc cgcgtctgct gccgtttcaa
    11521 ttggcaatgc acggaatgtc agggcttttt ttacagatgc cccgcctccc aattctttct
    11581 gccagttgtt aagtgaagag aatgttgaac tggtggttgc cgagcaggaa gtttcctgaa
    11641 attgaaactt cgaaaaatcc gctggggatt cagcggattt tattttgccg gatgcggcct
    11701 acggttgata cggttttcgt tggccggatt aagcacatcc ggccattaat taagcttcag
    11761 ccgccagcat ctctttctta cgttggctaa tacgcttaac cagcattacg gaaaccaccg
    11821 caccacagaa tgcgaagcag gccataatgc caaacacgat tttgtagcca ataatccccg
    11881 gatttaaatc cagaatgtag ccatacagac tgaagcagaa catcgccggg gcgtaaccaa
    11941 taaagctacc caacgccatc gccgcgcctg ttttattttc agcaattttt gcttcgccga
    12001 taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg
    12061 ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag
    12121 cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg
    12181 aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt
    12241 aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg
    12301 tcaggccaca gtaaaccgcg taaacgaaga agacgttaaa agcgataagc cagatagttt
    12361 tgtctttcag caccgaggtc atactggtgt ttttcgatgc tccgtcttct tttttcacct
    12421 cgacggacgg tgcctcttct ttgtcattca gaacaaagaa aatgataatg cctacggcaa
    12481 tcacaataag ggaatagaac cagatgcctg ctttaaaacc caataagcca ctgccaaacc
    12541 aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt
    12601 cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga
    12661 gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga
    12721 tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg
    12781 agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaaac
    12841 ccactgtcgt gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat
    12901 ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg
    12961 taccgccaca cagtgttaat agactaaata aggcccattt ctttttcgtg agcataacat
    13021 cccctaagcg atttataaaa tcactgtttt aaaggcaata agatatcgac cattcttatg
    13081 gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa gatggcttgc
    13141 ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa tcgcatcttc
    13201 tttttcttta cgtaaccgcg catccagtaa atgattttct ggtagtggtg tttcactcat
    13261 ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg ccggagcata
    13321 ataatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cgcccaggca
    13381 gataaggtgc gttaataacg ctttgaaggg catatctccc tgacctgaaa tacaggcttt
    13441 atggcccaga ccaccctgtt ctttaacaat caatgcatct ttgatatgca cctgggtaat
    13501 atgcggcgcc atcgttttta aagcgtcgat gggatgctca tttgcattga tcatgttcgc
    13561 aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccaacg acaccagctc
    13621 atggcttttt aaatcttcat gctgctcgat cgtaaaagtc aggccgctgt cctgatacgt
    13681 ttcccgtaca taggcaatgt cgttagcgat gatcgataat acgtcgcgca aattaccttc
    13741 ataacgtgga taaaaacgta cggacgatgc cccagttttc aacgcgatgg cgacggcttc
    13801 gtcaatagat gccttatctg aggcgctggt ttcaatatga atatcaaggt tcagacggcg
    13861 ggctttatca ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc
    13921 tccatccaga acatgaattt tgacgccccg cagattattt tcactcgcga tatcaagtaa
    13981 atcagcaggc aatactcttt ccagccgcat atttaaatga aaggcatagg catgtaaata
    14041 aagaggaaga gtatcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa
    14101 acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct
    14161 ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg
    14221 cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt
    14281 tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt
    14341 caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa
    14401 ctttatgtcc ttccaacgcc ccccagccgg tgatgcgata gagttcagtt gatggcgtca
    14461 ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggcttttc tcggtaaacc
    14521 cccaggaagg ttcccgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg
    14581 ggctgtaatt aaatcgcggc tgagtcacga accacccttt acgatcctta cggaagattt
    14641 tggattctgc ctcgaggttc agcaacgcct ggcgcagcgt catacgttta atgcccagca
    14701 gctctcccag ctctctttct gaagggagtt ttccacccgg cgtagtgatg ccatctttta
    14761 accaccgatc cagtttctct tttgcatttt ctacggtgga ttgatttccc gtcattgttt
    14821 aatccttttg gtttaaacca gatgaggcat cttacgctta cgccaaaaaa gaaagcaatt
    14881 aacggcgtat gactttgcag ataattgtga caaagctctc tattgacgta accacagact
    14941 tataagggag atactcgcat tgctgagtgc tcacgacagg caaaaaaaac ctgccatcgc
    15001 tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct ttcggtgcgc ggttggcgcg
    15061 acgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg gggtcacttc
    15121 taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc ggataggcgg
    15181 aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt tcttaccggt
    15241 caggcagttt acagtcaggt cgttagagcg gctatgaata ccgataatct gaccttcgta
    15301 aa
//
LOCUS       DACAPB010000108        15123 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.108, whole genome shotgun sequence.
ACCESSION   DACAPB010000108 DACAPB010000000
VERSION     DACAPB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.108"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..2209)
                     /gene="dnaE"
                     /locus_tag="IT904_002967"
     CDS             complement(<1..2209)
                     /gene="dnaE"
                     /locus_tag="IT904_002967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP3192716.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGI"
     gene            complement(2246..2842)
                     /gene="rnhB"
                     /locus_tag="IT904_002968"
     CDS             complement(2246..2842)
                     /gene="rnhB"
                     /locus_tag="IT904_002968"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP3192717.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPYEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            complement(2839..3987)
                     /gene="lpxB"
                     /locus_tag="IT904_002969"
     CDS             complement(2839..3987)
                     /gene="lpxB"
                     /locus_tag="IT904_002969"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAP3192718.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(3987..4775)
                     /gene="lpxA"
                     /locus_tag="IT904_002970"
     CDS             complement(3987..4775)
                     /gene="lpxA"
                     /locus_tag="IT904_002970"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAP3192719.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(4779..5234)
                     /gene="fabZ"
                     /locus_tag="IT904_002971"
     CDS             complement(4779..5234)
                     /gene="fabZ"
                     /locus_tag="IT904_002971"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP3192720.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(5339..6364)
                     /gene="lpxD"
                     /locus_tag="IT904_002972"
     CDS             complement(5339..6364)
                     /gene="lpxD"
                     /locus_tag="IT904_002972"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAP3192721.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(6368..6853)
                     /gene="skp"
                     /locus_tag="IT904_002973"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(6368..6853)
                     /gene="skp"
                     /locus_tag="IT904_002973"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAP3192722.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(6975..9407)
                     /gene="bamA"
                     /locus_tag="IT904_002974"
     CDS             complement(6975..9407)
                     /gene="bamA"
                     /locus_tag="IT904_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAP3192723.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(9437..10789)
                     /gene="rseP"
                     /locus_tag="IT904_002975"
     CDS             complement(9437..10789)
                     /gene="rseP"
                     /locus_tag="IT904_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAP3192724.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(10801..11658)
                     /gene="cdsA"
                     /locus_tag="IT904_002976"
     CDS             complement(10801..11658)
                     /gene="cdsA"
                     /locus_tag="IT904_002976"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP3192725.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(11671..12429)
                     /gene="ispU"
                     /locus_tag="IT904_002977"
                     /gene_synonym="uppS"
     CDS             complement(11671..12429)
                     /gene="ispU"
                     /locus_tag="IT904_002977"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAP3192726.1"
                     /translation="MLSAIQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(12618..13814)
                     /gene="ispC"
                     /locus_tag="IT904_002978"
     CDS             complement(12618..13814)
                     /gene="ispC"
                     /locus_tag="IT904_002978"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAP3192727.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(13906..14463)
                     /gene="frr"
                     /locus_tag="IT904_002979"
     CDS             complement(13906..14463)
                     /gene="frr"
                     /locus_tag="IT904_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP3192728.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(14755..>15123)
                     /locus_tag="IT904_002980"
     CDS             complement(14755..>15123)
                     /locus_tag="IT904_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP3192729.1"
                     /translation="AEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVFNMN
                     KPGALRRVVMGEKEGTLITE"
BASE COUNT         3649 a         4262 c         3600 g         3612 t
ORIGIN      
        1 tgattccgtt cttttctgca ccttcagcaa atacagaacg ctgcttagcc atctcttccg
       61 gctttttctt acccatcgca cgacgcagca tatccgcgcc accgagggta taaccagaaa
      121 gcacctgcgc aatctgcatg acctgttcct gatacaggat gatgccgtag gttggctcca
      181 gtaccggttt caggctttca tgctgccact gtacgtccgg ataggagatc tcttcgcgac
      241 catgtttacg gtcgataaag ttatccacca tccctgattg caacggacca gggcggaaca
      301 gtgccaccag tgcgatcata tcttcgaagc agtcaggttg cagacgcttg atcaggtcct
      361 tcatgccgcg cgattcaagc tggaataccg ccgtggtttc cgagcgttgc agcatgtcga
      421 agcttttctt gtcatccagc gggatcgcgg cgatatccag cggcggctcg ccattcttcg
      481 cccgccgctt gttgatcatc tccagcgccc agttgatgat ggtgagcgta cgcaaaccaa
      541 ggaagtcgaa cttcaccagt ccggcgtatt caacgtcgct tttatcaaac tgggtgaccg
      601 gatgtttgcc ctcttcatcg caataaagcg gcgcaaaatc ggtaattttg gtcggcgcga
      661 taaccacccc accggcgtgc ttaccggcgt tacgggtgac cccttccagt ttgcgcgcca
      721 tgtcgatcag cgccttaact tcttcatccg cttcgtagat ttccggcagc tgcggctcgg
      781 cttcaaacgc tttcgccagc gtcatgccag gatcgggcgg gatcagtttc gagatacggt
      841 cgacaaagcc gtacggatgc cccagcacgc ggcctacgtc gcggatcacc gctttcgccg
      901 ccatcgtacc gaaggtgata atctgcgata ccgcatcacg accgtacatg tccgctacgt
      961 gctcgataac ctggtcgcgt ttctccatac agaagtcaac gtcgaagtca ggcatggaga
     1021 cacgttccgg gttaaggaaa cgttcgaaca gcaggtcaaa ttccagcgga tcgaggtcgg
     1081 tgattttcag cgcgtaggcc accagtgacc ctgcaccaga accacggccc ggccctaccg
     1141 gtacgccgtt atctttcgac cactggataa attccataac gatgaggaag tagcccggga
     1201 agcccatctg attgataacc tgaagttcag tctccagacg ttcgtcatat tccgggcggc
     1261 gcttaacacg ttcttcctcg tcagggaata aaaaggccag acgctcttcc aggccctctt
     1321 ttgcacgctt gaccagataa tcttcggtgc tcatgtcccc ggtcgggaac tgcggcagga
     1381 agtattcacc aagacgcaca gttacgttac agcgtttggc gatctcaacg gtgttggcaa
     1441 gggcttctgg gatgtcggca aacagctcgc acatctcctc ttcgctacgc atatattgct
     1501 gcggcgaata gttacgcggg cgtttaggat cgtcgagggt aaagccgtcg tggatcgcga
     1561 cgcggatttc gtgtgcgtca aagtcgctgc tgtcgataaa gcgcacgtcg ttggtcgcca
     1621 cgacgggcaa accgcgcgct tccgccagtt ccaccgccgc gtgcaaatag ctttcttcat
     1681 ccggcctgcc ggtgcggatc agctcgagaa aatagcgatc cgggaagtgt tcttcataaa
     1741 acgcgacaca ctcatctacc agcgcgctgt taccacgcaa aagactgcgt ccgacgtcgc
     1801 ccatgcgccc gccggaaaga aggatcaacc cttcgtttaa ttcgataagc cagtcgcgat
     1861 cgatgatcgg cccggcggca ccgtacccgc gctgatacgc ttttgagatc agcaacgtca
     1921 gattctgata cccggtattg ttcgccgcca gtaccgtcag gtgggttaac tcatcaccca
     1981 gcaggtcgca ctggacatta aaatctgccc cgacgatagg cttaatccct gcgccatgtc
     2041 ccgctccgta gaacttcacc agaccgcaaa ggttggtgaa atcggtgatc gccagtgctg
     2101 gcatacccaa cgccgccgcc ttttttacca gcggtgcggt tttggccagg ccatcgatca
     2161 tcgagtagtc gctgtgcacc cgcaggtgta cgaaacgtgg ttcagacatc ttcagattcc
     2221 ggtttactta atctcgacac aagaatcagg acgcaagtcc cagtgcgcgt ttgacaggcc
     2281 caaagctgcg acgatgatgt tcagtcgcgc catattcagc cagtttttcc agatgaaaag
     2341 cggttggata ccctttatgt tgggcaaaac catattgcgg gaaaacaata tccagcgccg
     2401 ccatttcggc gtcgcgcgtc actttcgcca ggatagacgc ggcactgatt tccggtacgc
     2461 ggctatcgcc tttcaccact gccatcgaag gcatcggtaa tttcgggcag cggttaccat
     2521 caatcaacac atattccggc gcaatatgca gcccagcgac ggctcgctgc atcgccagca
     2581 tggtcgcatg aagaatgttc agctcgtcga tttcgtaggg ttccgcgcgg cccagactcc
     2641 agctcaacgc tttctctttg atctcttcac agagcgccag acgacgtttt tcgctcagct
     2701 ttttggaatc attcagcccg gcaatcgggc gcgccgggtc gagaatcacc gcagcggtga
     2761 cgaccgcgcc aactaacggc ccgcgtccga cttcatccac acccgcaacc aggtgcgtgt
     2821 gcggataaac aaattcgatc attgtgctaa ctccagaacg gcttgtgccg cctgctcatc
     2881 ggcattgcag cggatctgct gatgcagttc acggaaggta tcgtgcatcg cgtggctggt
     2941 tttcccgttc gccaacagcg gtaacagcgc cgcagccagt ttttgcggct cacactcttc
     3001 ctgcaataac tctttgacta actctctgcc cgccagcaga tttggcagcg agacataatc
     3061 agttttcacc agccgcttcg ccaaccagaa ggtaaaaggc ttcatgcgat atcccaccac
     3121 catcgggcat ttcgccagca tacactccag ggctgccgtc cccgacgcca gtagtgccgc
     3181 atcgctggcg accatcgcct cacggcccat tccatccagc aaatgaaccg acaggtctgg
     3241 cgcgacttca gctttgatgc gttcaaactg ctcgcggcgt ttggcattca ccagcggcac
     3301 cacgatttcg agatccggat atgtctggcg caaaagctgg gcagttttca ggaaatcggc
     3361 actaagcatt tcgacttctg caccacggct gcccggcaac aatgccagac agtgggcatc
     3421 gtaagggatc cccagcacat cacgggcacc atttttatct ggatctaacg gcatggcatc
     3481 agccatggta tgaccgataa agcggcacgg tacattgtat ttgtcataaa acgctttttc
     3541 gaaaggcaga aatgcgagca ccagatcggt ggctctgcct attttgaaaa cgcgtttctg
     3601 tcgccacgcc cagacggatg gactgacgta atgaatggtt ttgataccct gctttttgag
     3661 gttaccttca agagtaatat tgaagtcggg cgcatcaata ccaacaaaaa catctggctt
     3721 cagttcgcca aaacgctttg tcagatcggc acgaatatgc agtaagcgac gcagacgacc
     3781 gagcacttca acaatgccca tcaccgccag ttcttccatt tcgtaccagg cttcgcagcc
     3841 ttcagcctgc attcgtggcc cggcaacgcc aacaaagcgg gcgttgggca cacgttcttt
     3901 cagagcgcgg attaaaccgg cccccaggat atcgccggag gtttctccgg cgaccagggc
     3961 aatcgttaat ggacgctgtt cagtcattaa cgaatcagac cgcgcgttga gcgtgcaaag
     4021 aaatcggtaa aagctttcac ttccggatac gtttccgcca gttcagcaat ttccggtttc
     4081 acttcatcga gcgttttacc gctgcgataa atcagcttat acgcattgcg gatagcggta
     4141 atcgcctcgc ggctgaatcc gcggcgcttc agcccttcga tattgacacc gaacggcgtt
     4201 gcgtggttac cctgcgcaat gacataagga gggacgtcct gcgccacacc ggagcaaccg
     4261 ccaaccatca catgcgcacc aatgatgcag aactgatgga ctgcggtcat gccgccgatg
     4321 atcgcgaagt cgtctaccga tacgtgaccc gccagcgttg cgttgttggc gagaatacag
     4381 cggttaccta ccgtacaatc gtgcgcaatg tgcgcgttga tcatcagtaa gttgtcgctg
     4441 cccaccttcg tcaatccacc gccctggact gtgccacgat gaatggtgac gctttcgcga
     4501 atgcggttac gatcgccgat ttccacacgg gtcggttcgc cagcatattt cagatcctgg
     4561 ttaacttcac cgatggaggc gaactgataa atctcattat cgcggccaat tttagtatgg
     4621 ccattcacga caacgtgaga tttcagtacg gtaccctcac caatttcgac atggggtcca
     4681 acgatacaaa aaggaccaat gtgtgcgttc gcgccaatcg acgcgccctc ttccacaatg
     4741 gcggttggat gcacaaaggc ggatttatca atcacgtatc aggcctcccg gctacgagca
     4801 cacatcatcg ttgcttcgca aactacttta ccgtcgacca gagcaacccc tttaaaacgg
     4861 gtcaggccgc ggcgcgtttt ttcgaaagtg acttccatga tcatttgatc gccaggcacg
     4921 accgggcgct tgaagcgcgc ttcgtcaata ccagcgaaat agtacagctc acccggttcc
     4981 agttttccta cgcttttaaa cgccagaata cctgttgcct gtgccattgc ttccagaatc
     5041 agcacacccg ggaaaatcgg ttttccaggg aaatggccct ggaagaatgg ctcattgaca
     5101 gagacatttt ttactgcgcg cagaaaacga ccttcttcaa aatccagcac gcgatccacc
     5161 agtaagaaag ggaaacggtg cggcagaagt tctaaaatct cttcaatctg cagagtatga
     5221 gtgttagtag tcaagatact cttcctgtca aaatataaga aacgacaata ataacacggc
     5281 ctgccgcaat cgtaagaatg agacaggccg taaagtttgg cgaacaaaag atggaacgtt
     5341 agtcttgttg attaaccttg cgctcaagcg atttcagacg cttgctcatg tcatcaatgt
     5401 tcatcaccag tgcagcggtt ttgcgccaga ctttgttggg ttgcagcgga atgcctgagg
     5461 aatagacgcc tggttcagtg atgggacgca tcaccatacc catgcccgta accgtcactt
     5521 tgtcgcatat ttccatatgc ccgttgatta cgctggctcc gccgatcatg cagtaacgac
     5581 caattttcag gctgcccgcc ataatgacgc caccggcaac cgccgtattg tcgccaatca
     5641 cgacgttatg tgcaatctgg cactggttat caatgatcac gccattgcca ataatagtgt
     5701 catccagcgc gccgcgatcg atggttgtgc aggcaccgat ctccacgcga tcgccaataa
     5761 ttacgcgacc aatctgtggg atcttcaccc agttaccacg atcgttggca taaccaaagc
     5821 cgtctgcgcc taccactgtt ccggactgga tcaggcaatt ctgaccgatc tggatctcat
     5881 ggtaaatggt tacgttcgcc cagagacgcg aacctgcacc gattttgctg tttttaccta
     5941 cgaagcaacc ggcaccgata atcacgttat cgcccagttc aacgccggac tcaatcaccg
     6001 cgttagcgcc aatcgatacg ttgttaccca gcttcgccgt cgcgtcgatc accgcactgg
     6061 gtgcaatgtt ctgcgcgggc tgcggcgtgg tatctaaaat ttgcgccatg cgcgcgtaag
     6121 tcaggtaggg attcttcact accagcgcgg cacttttcgc gaaaggaaga tcgtcctggg
     6181 tcatgacaac cgcggacgcc tggcacaagc ctaaatgctc acggtatttt gggttaacca
     6241 tgaacgtaat gtgacctgtt tgtgcagatt gcatggacgc aacgccggtg atgacgatat
     6301 cgccatcacc gtgtagttct gcatccaact gctgcgctaa atcagccagt cgaattgaag
     6361 gcattactta tttaacctgt ttcagtacgt cggcagtgat gtcttttaca tcgctgctgt
     6421 tgtaagcaac ggcgtttgca tcaacaacca gatcgatatc ctggctgttg gcaacggatt
     6481 tcacagcagt ctggatacga gtaaccagtt tgccgcgttc ttcgttggaa cgacgtgcgc
     6541 gatcctgctc aaaagcctgc gctttctgag caaaagtctg gcgctgagcc atcacgtctt
     6601 tttccagctt agtgcgatcg ctgcccgctt tcattgactg cagctttttc attttagcct
     6661 gcagatcggt ttccatacgc tgcagttcgc tggcacggcc tttgaactca ttttccagcg
     6721 tgttagaaac accggttttc tgcgctacct gctggaacag gctgcccata ttgacgattg
     6781 caattttgtc agccgcctga gcagaagttg ccagtgctaa accgagacct gcagctaata
     6841 accacttttt cacaataaac tccttaccat cccatttgca ccggagggtg cagttctttg
     6901 cgtggcccgg cgatcttata ttgatcgcct aaagtcatcg ctacactacc actacattcc
     6961 tttgtggaga acacttacca ggttttaccg atgttaaact ggaactgttc tgccttgtct
     7021 ccatcgtact ttttgaacgg ctgggcgtag gagaacacca acggccccaa tggggacatc
     7081 cattgtaatg cgatacccgc agacatacgg atattgcttg gatcactata gtccggatat
     7141 ccagaatatt ggctggaatc ccagtttgta tcccaaacgg tacccatatc ccagaagaag
     7201 gaagtacgaa ccgagttagc atacttgtcg ctaatgaacg gcgtcggggt gatgaactcg
     7261 aggctggcaa ccgccatagc gttaccgcct acagcatcat ccgatttaca caggtctttc
     7321 gcgccgtcct gagtcgcaca ttcgtaatca tagtccggat cataattact ggcctgatgc
     7381 gggaagtaaa ctgctttcgg accaatggta ttggactgga agccacgcac ggtgctggaa
     7441 ccaccggcat agaagttctc gtagaacggc atctctttgc cgcctaaacc atcaccataa
     7501 ccccagcggg tacgccccag aacaacccat ttgtgatcgt catcgatcgg cacataagtc
     7561 gccgtgtcta acgtcacttt gtagtattcg ttatccgatc caggaatggt cactttaccg
     7621 gtcaggttga cacgtgaacc atctgtcggg aagtaaccac ggtcaagctt gttataggtc
     7681 caaccatagt tgaacgtgaa gtcgtccgtt ttgaagctgt tatcctgatc agaggtgctc
     7741 ggatgttcac ccatagagta cagataacgc cacatcgcaa cctgaggctg catgttggac
     7801 agggagttat gtacataacc cagacctgca cgcagcgagt tatattcgtt aatcgggaag
     7861 cccaacgtca cgtctgtacc ataactcttg ttggtatagt cggacaggtc ggcatcatct
     7921 gcctggaagt cattatagaa gagacgacca ccgaggctta cgccatctac ggtgaagtac
     7981 gggttggtta ccgacagttc agcataggtc tggtaatcgt ttttggtccc gttgatacca
     8041 acagcataac ctgtacctaa ccagttatcc tgttgcacac cagcctggaa gctcacgcca
     8101 ctttcagtac cgtaaccaat accaaagttg aagctaccgg tgttgcgctc ttttaccttg
     8161 tagactacat caacctggtc cgggctaccc ggaacacgtt gggtatcggt atcgacagtt
     8221 tcaaagaagc ccagacgatt cagacgctcc ttaccctgat cgaccagatc gctccccagc
     8281 catgcacctt ccatctgacg catttcgcga cgcaggacgg catctttcga ggtatcgtta
     8341 ccttcaaaac ggatcttacg cacgtagaaa cggttacccg catcaacgtt cacgcgtaat
     8401 ttaacggttt tgtcggcatc gttaatttca ggcatcgact gtacgcgcgg ataggcataa
     8461 ccatagcgac cgagaagctt tttaatgtca tcttccatct tggtcacttt ggtgccgtta
     8521 tacagctcgc ccggctcgat cttagtcagc tgctcaattt cagcggagtg cccggcaaga
     8581 ttgccgctca cttcaacgcc agaaagcttg tactgatcgc cttcggtgat gttcaccgtg
     8641 acgtaaatac cttttttatc tggcgtcaga ctgacctggg tagagtcgat gttgaaacgg
     8701 gcataaccgc gatccagata gtagctgcgc agggtttcaa ggtcgcccgc cagtttctgt
     8761 ttctggtatt tacgatcgcc taccacgttc caccacggca cttcgtcacg cagttggaaa
     8821 tgagagatca gttcgtcggt ggtgaaagca tggttaccaa caatgttaat ttgctggatt
     8881 tcagctgaca caccttcctg gaacaccagt tttaggtcaa cacggttgcg cggcagcggg
     8941 gtcacgacag ctttaacgct ggcgctatat ttaccgacgc tgtagtagaa gtcttccaga
     9001 cctttctcga tatcggcaat ggtggtgcga tcaagggatt cgcccacacg cacaccagaa
     9061 gcctcgaggt tttgcttcag catgtcatct ttcaccgatt tgttaccgga gaaagtaatg
     9121 ctggcaatgg tcggacgttc ttttacctga accagaaggg tatcaccatc acgaaggacg
     9181 cgaacatcct caaagttgcc ggtagcaaac agagcgcgaa tggtattact gatatcttca
     9241 tcattaaccg tgtcgcctgt gcgcaccggc atactgagga gggccgcacc aacggcgaca
     9301 cgctgaaggc cttcgaaatg aatatctttc actacgaacc cttcagcacc gtatacggtg
     9361 gcgctgctaa acagcagcga cgctatgagc aactttttca tcgccatcgt tattatgcgt
     9421 tcttcctaac taactctcat aaccgagaga aatcattgaa aagtgcaagc cccattaaca
     9481 gcaccagcaa aatcgagcca atgcgataac aaaagtcttg aacccgctcg gataccggtc
     9541 cgcccttgat cttttcgatc gcaaggaaca gcagatgccc cccgtcaagt acgggcaacg
     9601 gaaacaggtt aattatccct aagttcacgc taataagcgc aagaaacggc aggtaataaa
     9661 caaccccgag ttccgctgtc atcccagccc ccttggcgat agagatcggc ccactgaggt
     9721 tgttcagttt cacatcaccg gtgatcaatt ttcccagcat actgaccgtc agcttcatca
     9781 gctgccacgt tttgtccgtg gcttcgacaa tggcgttgaa cggcccatac tggcgtacaa
     9841 ctttatactc atctggcaaa ggaatgactt tcggctcaat accgacaaaa ccaatcgctt
     9901 taccattacc cggtttactc tccgggatta atgtcaaaga caagggactt ccctgccttt
     9961 cgatttctaa cgctaaggat ttacccgggt tatcccggac aagcatcaca aaggtcaccc
    10021 actgcgttaa gggctgacca tcgactttaa cgatcctgtc gcctgcttgc aaacctgcct
    10081 tgcttgccgc cgagtttggt tgcacatttt ccagtacagg ttcaatttgc ggcccacgag
    10141 gacgaatccc cagcgaagat accggatctt ctttatcagg ctcaaacgcc cagtgacgta
    10201 aatcgagctt tacatcccgc cgttggtcgc tgccaaatgg cgctactgta atggtggtgc
    10261 tttcatcgcc aattttatcg accaactgca aacgcacggc atcccaatca ggcgtttcga
    10321 taccatctac ggcttttagt tccgtacctg gtgcaatttg tgcttccgca gctatcgaat
    10381 tggctgctat ttcgccaacc accggacgta cgccaggcac accaataata aaaaccagcc
    10441 agtaggcaaa gatagcaaaa atgaagtttg caaccggacc tgcggcaata atcgccgctc
    10501 gttggccgac agatttatta ttgaaggcat ggtggcggag ttccggaacg accggttctg
    10561 cgcgctcatc cagcattttg acataaccgc ccagcgggat cagggcgata acatattcgg
    10621 tgccgagctt atcagttcgc cgccagagcg ccttaccaaa ccctattgag aaacgctcaa
    10681 cgcgaacacc acaacgccgg gcaacccaga aatgaccaaa ttcatgcacg gtgataagta
    10741 cacccagtgc aacgatgaac gaagccaaat cccagagaaa actcagcata ttaccttccg
    10801 ttaaagcgtc ctgaatacca gtaacaacaa gcaagcaaag accggtaccg cagccgtcag
    10861 gctatcaata cgatctaaaa taccaccgtg tcctggaatt aaatgaccgc tgtccttaat
    10921 tcctgcttca cgcttaaaca tactctcggt cagatcgccg agcactgagg ctaacgctgc
    10981 gacaatagag caaatgagta aggtgacggg agcgacgtcg agattcgccc acatgccata
    11041 accccatgag attactgctg cggtcgcgag tccacctata aagccttgcc aggttttacc
    11101 cggagaaacc ttcggtgcca gcttatgttt accaaacaat ttgccaaaca tatatgcgcc
    11161 agagtcagcg ccccatacca ggatcatgac atagagcagc catattgcgc cactgtaatg
    11221 attctcgtca tagtgccagg cccgtaacgc cagcatgccc cagaagaagg gaacaatggt
    11281 tagcacgcca aaaataaggc gcaatgtttt agagttacgc cagattgctg cggaacctgg
    11341 gtaaaacagc accagcaata gcgcgacaat ccaccaaccc agcgaagccc aaagtgagat
    11401 ttcaaccagc ggttgatgaa tatttcggtg atattccggc aacagaaaaa gcatcagcgc
    11461 caacaataac ccgcataaca ccgccaacca tactcgctgc gaacgagtgg taaaaccgct
    11521 aagctgtccc cattcccacg ctgccagcat gcagaccacc agcgttacga tggcgaaccc
    11581 caccggcggc aacagaaaca acgccgcgat gacgacgggt attaacacaa aagcagatat
    11641 caggcgatac ttcagcaaaa gcgaccccca tcaggctgtt tcatcaccgg gctcggtgcc
    11701 gccgaaacga cgctctcgat tagcaaaggc atttaacgcc ccttcaaagt cttgttcatc
    11761 gaaatcgggc cagagaacat ctgtaaagta aagttcggca taggcaattt gccaaagcaa
    11821 aaagttacta atgcgatgct cccccccagt cctaattact aaatctacag gggccagttc
    11881 atgcatacag acatgctggt ttagcatctc ttcatctatc tgatctggtt gcaggtttcc
    11941 ttgctgcacc ttttcagcca gttgcctgac tccctggact atatcccaac gtccaccgta
    12001 gttcgccgca atattcagcg tcagaccggt attcccggct gttagcgctt cagatttacg
    12061 aatacgttct tgcaaacgcg agttaaagcg actggtatcg ccaataatac gcagacgcac
    12121 gttatgtcgg tgcagacttt ttacttcgct atcgagcgcc cacacaaaca gttccattaa
    12181 cgcactgact tcctgcgctg gtcggttcca gttttcacta ctaaaggcat acagcgttaa
    12241 cgcctcaata ccgttgttgg ccgcaaaaga gacagcccgg cggacggatt ttgccccggc
    12301 tttatgccca aaggctcgaa tcttcccttg cttttttgcc cagcgaccat tgccgtccat
    12361 aatgatcgca acatgacggc agccatgcgc tggcaatttt tcgctaagtg gttgaatagc
    12421 agacaacatc acgcgttttt tattccctga cagggtttac ggtactcagg aatactgaag
    12481 cctatacaaa aaaaagccgt gtaaaaccac ggcatacctg accttataaa gccaactact
    12541 tacgatcaga tggcgcagac tatatcactg aagccctacg ctaacaaata gcgcgactct
    12601 ctgtagccgg attatcctca gcttgcgaga cgcatcacct cttttctggc gacttcacgc
    12661 gcgttcgcat caacagataa cacatcgtcc acacattgtg gttcgcgcat atccattttt
    12721 tccagtaccg ataaattcaa cgcagcgata tccgtaaagc ggatttgttg cgcaagaaaa
    12781 gcagcaacgg tgatttcgtt tgcggcattc aatgctgtcg tcgctgcctg accttgttcg
    12841 aacgcctcca tcgccagttt caggcatgga taacgatcat aatccggtgc ggcaaatgtc
    12901 aacgcactta gtttgcaaaa atcgagcggc ttcacgccag agttcacgcg attcggccat
    12961 gccatggtgt gggcaattgg cgtacgcata tccggttccc ccagctgcgc cagaacactg
    13021 ccgtcctgat agcgcaccat tgagtgaatc actgactgcg ggtgaatcag cacttccatc
    13081 tggctggcgc tggcgttaaa cagccaacgc gcttcaatgt attccagacc tttgttcatc
    13141 atggtagccg aatcgacaga aattttacgc cccatcgacc agttcggatg acggcaggct
    13201 tgatccggcg tcattgttgc caaatcgcgc aatggcgtct cacggaaagg gccaccagac
    13261 ccggtaagta aaatggacac cacgccattt tgctcaaggt cagcgtatcc cagattatgc
    13321 tggataggtt gcggtaaact ctgaaaaatg gcgttatgtt cgctatcgac cggtaacaat
    13381 tgcgctttgc tctgctttac ggcgtccata aacagacgtc cgcaggtaac cagtgattct
    13441 ttattggcca gcaaaatggt tttacccgcg cggatcgcag caagcgtagg taacagccca
    13501 gcagcgccaa caatggctgc catcacctga tcaacatcct caagcgctgc catatcgcaa
    13561 gcggcttgtt gcccacttaa gacttcggtg cggctaccct gttgctgtag catcgtttta
    13621 agaagtttcg cactcgcttc atcgtccatt acggcatagc ggggagagaa ttccaggcac
    13681 tgttctacca tgcgagtgac atttttgcct gccaccagcg caactacgcg gaagtgttcg
    13741 ggattatggc gcaccacgtc cagcgtgctg caaccaatcg agccggtcga gcccagaatg
    13801 gtgagttgct tcatgaaaca tccagagttg agacagaata aaaagcaaaa cgccgccagc
    13861 cgatccgcaa ggatctactg agcggcgttt ttgtcgttca agaaatcaga actgcatcag
    13921 ttctgcttct ttgtctgcca gcgccgcttc aattttcttg attgcagcat cagtcagttt
    13981 ctgtacatcg tcctgagaac ggcgatcgtc gtcttcgctg atctctttat ctttcaacag
    14041 tgctttcact ttgtcgttcg cgtcacgacg cacgttacgt actgcaacac gcgcttgttc
    14101 tgcttcacca cgaacgattt tggtcagatc tttacgacgt tcttccgtca gcggcggcag
    14161 cggaacacgg atgtcgctac ccgcagagtt cgggttcagg ccgagatcgg acgccataat
    14221 cgctttttca acggccggag acattgaacg atcaaacacg ttgattttca gagtacggga
    14281 atcttctacc gttacgcttg ccagctgacg cagcggcgtc ggcgtgccgt aatattccac
    14341 gacaatgcca tccagcaggc tgggagaagc acgacccgtg cgtattttgc tgatttgggt
    14401 tttgaacgct tctacgcatt tgtccatgcg tacttcagca tcttttctga tatcgctaat
    14461 cacgttacga atccttgaaa acttgtctca gtcagaccag acagtcacac acagtggaag
    14521 tgtgctaagt atagccctga ttaaacatat tacgggtaat cgtctggatt attaggctat
    14581 tttatttgcc attttggccc cgagcagtgc tcgccatcct cacgtacttt tgtacgctcc
    14641 ggttgctgcg cgctggccgt gaccaaactg cctgcaacaa taacgcctta taaccagact
    14701 tacgcccccg cgatacttac gcggaatctt acccttattt atccatcacg ggaattattc
    14761 cgtgattaaa gtcccttctt tttcacccat taccacacgg cgcagcgcac ccggtttgtt
    14821 catgttgaaa acacgaatcg gtaatttatg gtcacgagcc agcgtgaagg ccgccaggtc
    14881 catgactttc agctcttttt ccagcacttc gctgtaagtc agttgctcgt acatggttgc
    14941 ggttggatct ttcgccggat cagcggtaaa cacgccgtca actttggttg ctttcagcac
    15001 cacatcggct tcaatttcga taccacgcag gcaagctgct gagtcggtgg taaagaacgg
    15061 gttacctgta ccggcggaga ggatcaccac acggttgttg cgcaacaggc tgatagcttc
    15121 tgc
//
LOCUS       DACAPB010000109        15032 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.109, whole genome shotgun sequence.
ACCESSION   DACAPB010000109 DACAPB010000000
VERSION     DACAPB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.109"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            86..160
                     /locus_tag="IT904_002981"
                     /pseudo
     CDS             86..160
                     /locus_tag="IT904_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(157..1479)
                     /locus_tag="IT904_002982"
     CDS             complement(157..1479)
                     /locus_tag="IT904_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAP3192730.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPLQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVKIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(1479..3095)
                     /locus_tag="IT904_002983"
     CDS             complement(1479..3095)
                     /locus_tag="IT904_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP3192731.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3391..4107
                     /locus_tag="IT904_002984"
     CDS             3391..4107
                     /locus_tag="IT904_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3192732.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNANEYQVEYHLRRLHPEKS"
     gene            complement(4104..5765)
                     /locus_tag="IT904_002985"
     CDS             complement(4104..5765)
                     /locus_tag="IT904_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAP3192733.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5961..6389)
                     /gene="ibpB"
                     /locus_tag="IT904_002986"
     CDS             complement(5961..6389)
                     /gene="ibpB"
                     /locus_tag="IT904_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAP3192734.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6501..6914)
                     /gene="ibpA"
                     /locus_tag="IT904_002987"
     CDS             complement(6501..6914)
                     /gene="ibpA"
                     /locus_tag="IT904_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAP3192735.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7220..7552
                     /locus_tag="IT904_002988"
     CDS             7220..7552
                     /locus_tag="IT904_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP3192736.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7554..8804)
                     /locus_tag="IT904_002989"
     CDS             complement(7554..8804)
                     /locus_tag="IT904_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAP3192737.1"
                     /translation="MMAGPVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWY
                     YDFHHRRGVIAESGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVM
                     QELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            8869..9969
                     /gene="cbrA"
                     /locus_tag="IT904_002990"
     CDS             8869..9969
                     /gene="cbrA"
                     /locus_tag="IT904_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAP3192738.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHISARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNEITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(9930..11266)
                     /locus_tag="IT904_002991"
                     /pseudo
     CDS             complement(9930..11266)
                     /locus_tag="IT904_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(11341..12489)
                     /gene="dgoD"
                     /locus_tag="IT904_002992"
     CDS             complement(11341..12489)
                     /gene="dgoD"
                     /locus_tag="IT904_002992"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP3192739.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIGGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12486..13103)
                     /locus_tag="IT904_002993"
     CDS             complement(12486..13103)
                     /locus_tag="IT904_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAP3192740.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13087..13965)
                     /locus_tag="IT904_002994"
     CDS             complement(13087..13965)
                     /locus_tag="IT904_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAP3192741.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCMESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREEKTPVVMAGMVGSNVGWKVAPYLSVPACFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFGVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13962..14651)
                     /gene="dgoR"
                     /locus_tag="IT904_002995"
     CDS             complement(13962..14651)
                     /gene="dgoR"
                     /locus_tag="IT904_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAP3192742.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
BASE COUNT         3741 a         4039 c         3816 g         3436 t
ORIGIN      
        1 ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag
       61 gacggggttt tacggcgcac cggataaatt tggttatgcg ccgtcagccg tgctaaagaa
      121 tacggaccag cacccaacgg atgccagttc acacaattaa cgtaactctg gccagtacgc
      181 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
      241 tttcgataac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
      301 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
      361 gccgaccacc agtggttctg gtccgttgtg cccgaccaga cacgggattt tgaccatcgc
      421 ttcatcatca aagttatgaa ttgccccgtt gttaggcaca atcagcaaca tccgctcctg
      481 agtgttgaag gcaattgccg ccgccagatc gacgatgtat gacgcatgtt cgtcaatttc
      541 cagcttgccc gccgctgaat ttcctgccgc cgtaatggcg cggcaagcat cgaaaacctg
      601 tttctcgcga tgttccatca cctcattcgc gcgggtatgt tcagggttgg aatgctgaac
      661 gacataatcc gggaagagat aatatttcag ataggtgttt ggtaatgtat ccgggtccag
      721 tgcctggaca tcccgcgctt ttgcataggt gtcgttccag ctcgcttcag tatgtgtatc
      781 ttgcagcggt ggaacataac cgtgcttaga gacatattgc cgcagctggg gcatcaggtc
      841 gttgccctgc aaatcttcaa ttgatgtcca ccagccaaag tggttcaggc cgtagtagcg
      901 cacgcgcatc tgtttgcgat cttgcagccc aacaatttgc gccatccggc tttcaatacc
      961 gattggcatg tcacagatgt tgaggatttt cgcattcggg cgcagacgac gcgtggcttc
     1021 tgcgacaatt gctgccgggt tggagtagtt gagcatccag gcgtttggtg aatatttttc
     1081 catataatcc accagttcca ggacgccgcc aatggaacgc atgccgtacg ctattccgcc
     1141 gggtccgcaa gtttcctgac caacaacacc gtggcgcagt gggatttttt catccagttc
     1201 gcgcatcggg tacttgccga cgcggatgtg cgccatgaca aaatcaacgt cgctgaatgc
     1261 cacttcagga tcggtggtgt aactaaacgc aatgtccggc gctttctctt taaggatgac
     1321 tttacaggct tcggcaatca cttcctgccg cgcaccatcg ttatcataaa atttcagtgc
     1381 acgaagcggg aaacggtcct gattcgccag gagcatcaac acgatgcctg gcgtaaaggt
     1441 gcttccaccg cctgcgacaa ccactgagaa tttggtcatt atactgcctc cgtaatggca
     1501 acattttctg ctgattgatg agaattaatt aagctatcga gctgttcacg cagctgggat
     1561 acatgcagac caatgatcac ctgaatggca tcgccactac ggaagacgcc gtgcgctccc
     1621 agctttttaa agacttcgtc atccagcgtt tgtgacatgt catgcagtgc aatacgtaaa
     1681 cgcgtcgcgc aattgttaat gctggagata ttgccgaccc cgcccagggc ttgcaggata
     1741 ccggcagcct gatccagctc tttttttggc tctgccgcgg tggtttggcc tcgcgaggct
     1801 ttgtattcgg cttttgagta gagtttcact tccgcatctt cacgtcccgg cgtgcacatg
     1861 ttgaactgca gaatcagtgt gcggaaaacc acgaagtaca gcagggtaaa gcacaaccca
     1921 atggcgattt gggtcagcat catatccgcg tggttgctga acatcgggat ccagttttgc
     1981 ggtaaaacct ggtcaatcag acctccgccc atgttgccca ccacaccaaa gagatacatt
     2041 acggtcgaca ttgaggccgc cagcacggcg tgtaccgcaa acagcaacgg tgaaatgaac
     2101 aggaaggtaa attccagcgg ttcggtaatt cccaccagca tggcggttaa ggttgcggga
     2161 atcagcaagc ccgccacttt tacccgattt tccggtgcgg cagtgaagta catcgctaaa
     2221 gaaatgccca cggcaccaaa gatttttgag ttaccgtgca gggcaaaacc gccttccggg
     2281 aacaacgatt tcagcggctc ggcgctcaaa ctgaactctt gcagatgctg cgcccagtac
     2341 atctgaatgc cgccttcaac agctgccgga ccaaagataa actgtccgta gatgaagtgg
     2401 tgtaaaccgg ttgggatcag aatacgttcg aggaaggtgt aaacccagac cccaagtgca
     2461 cccgccgaac gcaggaacgc ttgcagagat tcaatcccca tttgtacttt tggccagccg
     2521 agcaacgtca gccaggcaca ggggatcatc accaggaagg cgataatcac cacataagac
     2581 gtcccctgga aaatgccgag aaaaaccggc agttttttat cgaacagacg gttatgcagc
     2641 gccgtcacaa tgccggaaat gataattgcg ccgataatgc tggtatcgag ggttttaatc
     2701 ccggccatca ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc gacgccgaag
     2761 tagcttcccc aggtcattcc catcgcgttg atgaaatagt tccaggtcag gaaactcacc
     2821 atcaccgcca gacaagcacg cccctgcgct tgcttagcaa ggccaatggg taaaccgaca
     2881 gcaaaaatca gcggcatatt acggaatacc gtccaaccgc cctcttcaat aatgtgtacg
     2941 atttgcgcga ataaactgtt cggatcggtc agtgattccc cgacaaacat cgggttttgc
     3001 agcaagatgg caagacccac cacaatcccg gcgaagggaa acagcagcac tggcgtgaac
     3061 atcgcgccgc caaagcgttg aatttgactg agcatttcca catcctcttt tctcaattct
     3121 gtaagtagga agcctgattg gttatgcggc aggcctggag cgaggatagg aagatgcgat
     3181 cggcagaaca tgacgaccca atgggattcg tgatcgcttt catgcttttc gttgggcggt
     3241 agagatctac gttttcgcga aaaagtagac atctgtgagc actggtacac agcatcgaac
     3301 acatctttaa aaaaaagatg ttttttcaat cgattaagca gaacttgtgg gcgcattacc
     3361 cgggcttgca ggcaaaaaag agatctagag atgatctaca aaagcattgc ggagcggtta
     3421 agaattcgac ttaattccgc agatttcacg ctaaacagcc ttcttcccgg tgaaaaaaag
     3481 ttggcggaag aatttgcggt atcgcggatg accatccgta aagcgattga cctgctggta
     3541 gcgtgggggt tggtggtccg ccgccacggc agcggcactt acctggtgcg caaagatgta
     3601 ctgcatcaaa ccgccagcct gaccgggctg gtggaggtgt taaaacggca gggaaaaacg
     3661 gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc ctccggccat tgccagccag
     3721 ttacggattc aaatcaacga gcagatctac ttctcccgtc gcgttcgttt tgtggaaggg
     3781 aaaccgctga tgctggaaga cagctatatg ccggtaaaac tgttccgtaa tctttcgctg
     3841 caacatctgg aagggtcgaa gtttgaatat attgaacaag agtgcgggat tttgattggc
     3901 ggtaattatg aaagcctgac gccggtgctc gccgatagac tgctggcgcg gcaaatgaag
     3961 gtagcggaac acacgccact gctgcggatc acctcgttgt catatagcga gagcggggag
     4021 tttttgaatt attcagtaat gttcagaaat gccaacgaat accaggtgga gtaccattta
     4081 cggcgactcc acccggaaaa gagttaaccg atactccaga agagcaccgc cagtaattgg
     4141 ggggtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga gagcgccgct
     4201 gcaccgctgg ttggatgaag attattagca aatgccagcg ccggtggatc ggtcatggag
     4261 ccagccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg cgccagaata
     4321 ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca gcttagccct
     4381 tcgccattga ccagggtatt cacaaaatcc ccaccagatt tcagaccaac gaccgagagg
     4441 aacagcacga tccccagctc ccgcagcgcg aggttggcac ttggcggcat aaaccagtac
     4501 agcttgccaa tactgccgat acgcccgagg atcaacgcca taatcagcgg accgcccgcc
     4561 agccccagtt tcaacgcggc cgggaatccc ggcacaaaga cgggaataga gcctaacaat
     4621 acgccaagcc cgataccaat aaacaccggc aacatctgaa cctgttgcag tttttgctgc
     4681 gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac caggttgaga
     4741 atatcgccga actgcaggct gatatcgcca ctggcgacca gttcgacccc ggcacggttc
     4801 aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat acgttttccg
     4861 agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgcc tttcgtagac
     4921 agcgaggtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc cgctggctgc
     4981 cccaccagat gcagcaaatc gcccagttgg ataatggtat ctggcgaagg aacttttagg
     5041 gtttcttcac gtttcagacg cgagcagata attttgtcgc cgttgagaat aggtacatct
     5101 ttaatggcta aatcatgcag gttagggttc tcaacgcgaa tattgatagt cctgatcagc
     5161 gcgccgccat tggtgcgtga agactcgtgc tgctgggctt ctgtctcgac attgacgcgg
     5221 aaaataaccc gcaacatcca catggtgaac aatatcccgc aaatgccgaa tggatacgcc
     5281 atcgcataac tcatccccat ctgatcgacc atttccattg gtgtacccag gtcgcgcaag
     5341 atctgctgcc ctgcccccag cgctggcgta ttggttaccg caccggagaa aatccccagc
     5401 actaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag accaccgatg
     5461 atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag
     5521 aagcccggac ccacctgaat cccgatggta taaacgaaca ggatcaggcc aaattcctga
     5581 ataacatgca gcatatcgct acttaatgtc attcccgcct gagaaacaaa atggccgacg
     5641 atgatcccac caaacagcac gccgccaata cctaatccta tgccgcgaaa tttgacgttg
     5701 ccgataaaca aaccgacgac tgccaccaaa gccagaatac tgaccgttaa tgctatatca
     5761 ctcatcgtcc atttctcttg agataacatt ttaattgcta cggattatgg ctgaatcggt
     5821 gaagcagatt tgccttatag cgcacattat cacgggaaag aacgtgccga aatatcttaa
     5881 acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca ccagaatggc
     5941 ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat agcgatacgc
     6001 tgcgctgcga tgggttcagg ctcgttacga attaaatcaa tatgcagtaa accgtttacg
     6061 aaggttgcgc cagagacttc catattttca gccagcgtaa aactcaggct aaatggctga
     6121 ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg cgtgcctttt
     6181 acgctcaggc gcgtaccttc cagttgaatc tctaaatctt cctgacggaa acctgccaac
     6241 gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc
     6301 tggctttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat ccattgacgc
     6361 atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa gcgagtaagt
     6421 acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga tgtcaggccg
     6481 cgccaggcgg ccttagggaa ttagttgatt tcgatacggc gcggtttttt cgcttccgga
     6541 atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcgcc acgaacatga
     6601 atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga
     6661 taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag attatcctgg
     6721 gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg
     6781 ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg gctctggttg
     6841 ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag cggggataaa
     6901 tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac cttccacaat
     6961 ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcggctac gatgtaaaaa
     7021 tgggtctgga aatgaacttt tcaagcctta tcagcggact tttttttgca gtttatggtc
     7081 tattgcatag actgaggggg cagcacgaaa cgttaaagtg cgattgccgc cacagagcga
     7141 cgtaatgatg atgttatttt cagcaaggga tcgctataac tcatcaggcc aacacccacg
     7201 atcaacagat gaatttatta tgataagaaa tgtgttgtta gcgttcatga tatgcagcgg
     7261 aatgacatta ctcggaggat gctccagcgt gatgtcccat accgggggga aagaaggaac
     7321 gtatcccggc acccgcgcca gcgccaccat gattggcgac gatgaaacca actggggcac
     7381 taagtcgctg gcgatcctcg atatgccgtt taccgctgtg atggacacgc ttttactgcc
     7441 gtgggatgtg ttccgcaaag acagctcggt gcgctcgcgc gttgaaaaaa gcgaagccaa
     7501 cgcgcaagcc accaatgcgg ttattccacc ggccagaatg cctgataact gatttagcga
     7561 tcagtttcgg tgatccacag ctggccacct tccatccacg ccagccattg accatccggt
     7621 gagaagacca cggcatccgc agaaggcgga ttggcgtggt tttcggttaa atactcaacc
     7681 tcgccacttt gcgcatgggc acaagcaatt cgattatcca gcacaaagcc caaccattct
     7741 cctgacggat gccagttaaa tgcagactga atatccgttt tgttatgggt taactggcgc
     7801 ggctcgccgc cctgtggcga gataagccac agttgcacaa tgccgttatc atcacgcatt
     7861 aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg gacgttgact
     7921 aaccccggat aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac gccacgcggt
     7981 ggcgcgggca gggttgtttc cgttccgctt aacggcgcat cacctgccgc tttccagcca
     8041 gcttcatctt gcggtaactc aacgataaac agctccggca ctttctcgcc ctttggcgaa
     8101 agtgtgtcgc caataaatgc cagcgcgtga tttcctaccc atccttcttc ataagcacga
     8161 ttgatttcat cactgccagg ctgtggcgtg ggcgtggttt tgctcaccag cacgcaccag
     8221 tggctaccgc tgtattcacg cggatgctgc ttttgtacgt tgaccgggcc aaacggcgca
     8281 gcaacaccga cgtttcgcaa atccagcgcc ggatcgagtt cttgcattac atggtcgtta
     8341 taggtaaagc tcaccctttc accgttcggg ctaaagacat gcacatggct gccgccgcgc
     8401 agcgctcctg gggtgtacgg tgcagtaata tccattgcat cgagattgct caccttgccg
     8461 ctttcagcaa tcacgccgcg acggtgatgg aaatcgtaat accatgtttc atcaggattt
     8521 tccgggccgt gaataaagac atatttctct gactttggat gaacggtcac cacgccgacg
     8581 tgtgcgccct gtgacgcgcg atagataacc tcgacctcgc cggtatggat attcacacgc
     8641 tcaatggtct cgccggtaaa cgacgcgcca gaaggacgca cgtcaaatac cagccactgg
     8701 ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat tacggggagc aaaggttatc
     8761 tgtttcatgg cgcggtcctg atacaaaacg ggtcccgcca tcatacttca caaggacttc
     8821 actttcagac aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat ggaacatttc
     8881 gacgtggcga ttattggcct cggcccggca gggtcggcgt tggcacgaaa gttagccggc
     8941 aaaatgcagg tgatcgcgct ggataaaaag caccagtgtg gtactgaagg tttcagcaaa
     9001 ccttgtggcg gtctgctggc accggacgcg cagcgttctt ttattcgcga tggactgacg
     9061 ctccctgtcg atgtgatcgc caatccgcag attttcagcg tcaaaaccgt cgacgtcgcc
     9121 gcatcgctca cacgtaacta ccagcgaagc tatatcaata ttaatcgcca cgctttcgac
     9181 ttgtggatga aatcactgat ccccgccagc gttgaggttt accacgatag cctgtgccgg
     9241 aaaatctggc gtgaggatga taaatggcat gtcatttttc gtgcagacgg ttgggagcag
     9301 catatttccg cccgctatct ggtcggtgcc gatggtgcca actcgatggt gcggcgacat
     9361 ctctacccgg atcatcaaat ccgtaaatat gtcgctatcc agcagtggtt tgcagagaaa
     9421 catccggtac cgttctactc ctgcatcttt gataatgaaa taactgactg ttattcatgg
     9481 agtatcagca aagacggtta ttttatcttt ggcggtgctt atccaatgaa agacggtcag
     9541 acgcgtttca cgacgctgaa agagaaaatg agcgcctttc agttccagtt tggtaaggcg
     9601 gtgaaaagcg aaaaatgcac ggtgctgttt ccctcgcgct ggcaggattt tgtctgcggt
     9661 aaggacaacg cctttctgat tggcgaagcg gcaggattta tcagcgccag ctcgctggag
     9721 gggattagct atgcgctgga tagcgcagag attctgcgtg cggtgttact gaagcagccg
     9781 gagaagagca acgccgccta ctggcgcgcc acccgcaaac tgcgtttaaa actcttcggc
     9841 aagatagtaa aaagccgatg cctgaccgca ccggctttaa gaaagtggat tatgcgcagt
     9901 ggtgtggcgc atattccaca gttgaaagat tatccaacgc gcttcacatc gcccaccagc
     9961 aggatgtaag agagcgcgcc aatcaacgcg acggcggaga tataaaccag tgcaggtgcg
    10021 aaaccgtaac cctgcgccag gtaccccacc accagcggaa cggtgatgcc gcccagacca
    10081 ccggcgaagt taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc cagagaagag
    10141 accagcgacc aggtaatcga agcaaaaccg ttgccgaaga atgccagcgc catcaggcac
    10201 ataatcatca tcggatcgtt agtgtagtta gcgcccataa tgcaggtgga gatcagcaag
    10261 ccgcagataa tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg taccagcaga
    10321 tccgcgaccc agccagagag caggacgccg acaaacgccg cgaggaatgg caccgtggtc
    10381 ataaagcctg ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt cgggaaccag
    10441 gttaagaaaa accacagtgt agaagccacc gcaaattgcc caagatagac gccgatcagt
    10501 ttacgatgaa acaccagttt ccagtctttg gctgttaacg gctgacgtgc ctcttttttc
    10561 accggcgcat cgccatccac cagaccgccg ccatcacgaa tgtaatccag ttcagctttg
    10621 ctgatacctt tggtcaggcg cggcggctga taaaccttaa accaaatcag cgaccagata
    10681 atgccgatac caccgggaca atgaacaccc agtgccagct caacatctcc tgaatccaga
    10741 tcagcagcgg agtcagaaac gccagaccga caaactgacc agacgtataa aaaccaacgg
    10801 cagaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg gtcgggaaag
    10861 cgggcgcttc gaaaataccg gttatcgcgc gcagaccaat taatgacatt aatcccgtgg
    10921 caaagccctg gaataaagtc gccactgacc agccaaatat cgcaataaaa taagtcacgc
    10981 gagaacctac gcgatctaaa aaccaaccgc cggggatctg acatagcgta taaagccagg
    11041 cgaaggccga aaatacatag cccatttccg ctttggtaat accgaactct tcctgaatat
    11101 gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga
    11161 tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca
    11221 tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac
    11281 agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa
    11341 ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc
    11401 atttttactg aactcaataa ctttagcttc gtcgatttcc acgcctaagc ccggtttcgt
    11461 tagcggttta aagaagccgc caaccatgct gaagtcctct ttgtttttca caaagtcgag
    11521 taactccgcg cctttgttgt aatgaatgcc catactttgt tcctgaagta cggcgttata
    11581 ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgagcg gacagtgcgg
    11641 cgcaagggtc acatcatagg cttctgccat tccggcaatt ttgtagcatt cagtaatacc
    11701 gcctgcatgg gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacgcgttt
    11761 aaaatcgaag cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc
    11821 cagtttcggg tagtattcgg cttgttctgc cagcaccggc tcttcaataa acagcgggcg
    11881 atacggctcc agttctttaa tcagcacttt cgccatcggc gcgctgacgc gaccgtggaa
    11941 atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac
    12001 tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt
    12061 gaaggtatcg aagccgattt cgcgcagggt tttaatgccg ccgataacat ccgccggacg
    12121 atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg
    12181 ccagaccggc gcattcagca ctttaccttt gatatcccat aacgcctggt caatcccggc
    12241 gatggcgctc atcaggatcg gaccgccgcg atagaatccg gcgcgataca tcacttgcca
    12301 taagtcattg atgcgcgatg gatcctgacc aatcaaatag tcacccagct cgtgaactgc
    12361 cgcttccacc gtacgggcgc ggccttcgat cacgggctcg ccccaaccga ccacgccttc
    12421 atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt
    12481 gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg
    12541 cgctctacgg attgcccggc gcgatagaga tcgctgccta agcccgcccc tgcacaacct
    12601 gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg
    12661 tccgatggca ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga
    12721 aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg
    12781 cagccggggc agacggtcat gccgtagccc accgcacggc ggatcacttc actatggata
    12841 ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc
    12901 agtaccgtac ctgcgccaat caacgccttg tcgccgtacg catcaacgat ggcgggaatg
    12961 ctttgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc
    13021 accgcgccaa catgcgccag cgcctcgtcg ggcgtaatac cgcgcaaaat ggcgatcagc
    13081 gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc
    13141 gcccgccacc gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt
    13201 cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gccacatagt cgcgcatact
    13261 ggcgacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag
    13321 tgttcccagc acatgcgagg cgcgaactcc aaaaagctgc ggcaatatgg cgggcgtatt
    13381 aagaccacgc tcaaggccag ctgtgaaggc atcggcagag ttttcctgcg gcggcaaacc
    13441 tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg
    13501 aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgagttc cgggcatgac
    13561 ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat
    13621 cacattgtgg ttatcgtcat gagagacaca taatccggga ataatccaga tattgtcgcc
    13681 aactgacgtt aattgttcgc caatagacga aaaacaggca ggaacagata aatacggtgc
    13741 aactttccag ccgacgttgc tgccaaccat tcctgccatt accactggcg ttttctcttc
    13801 acgccagtcg gtcgtgactt ctgctaacac cgcagccgga gattttccgt tcaggcgcgt
    13861 gacgcctgct tctgattgcc tgctctccat gcagtggtcg ccctgataaa gccaggcgcg
    13921 cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac
    13981 cttcgtgtcg agctggcgat catggtaagc gccgcctgct ctgccgcatc gccgtcctga
    14041 tgtcgtatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca
    14101 tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt
    14161 tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct
    14221 tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc
    14281 tgcgccagat cgctggaagt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc
    14341 agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcatt ttccagcacc
    14401 cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg
    14461 cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg
    14521 atgttgcgcg aggttgcaaa ctcctcacag agttccgcct cagccggaag cggcgagcct
    14581 ggcacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta
    14641 ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat
    14701 gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gtgcgtgtaa
    14761 tgtagtacaa acttatattg ttgtactaca atttagatca caaaaagaac aatgcataaa
    14821 aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa
    14881 agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat gaagttgaat
    14941 tttaagggat tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt
    15001 atctcgtatg ctctggtttc ccataccgca aa
//
LOCUS       DACAPB010000110        15012 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.110, whole genome shotgun sequence.
ACCESSION   DACAPB010000110 DACAPB010000000
VERSION     DACAPB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.110"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(48..1640)
                     /locus_tag="IT904_002996"
     CDS             complement(48..1640)
                     /locus_tag="IT904_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAP3192743.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1859..2779
                     /gene="ldtB"
                     /locus_tag="IT904_002997"
     CDS             1859..2779
                     /gene="ldtB"
                     /locus_tag="IT904_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP3192744.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2838..3956)
                     /locus_tag="IT904_002998"
     CDS             complement(2838..3956)
                     /locus_tag="IT904_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAP3192745.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3953..4420)
                     /locus_tag="IT904_002999"
     CDS             complement(3953..4420)
                     /locus_tag="IT904_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAP3192746.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4606..4734
                     /gene="mntS"
                     /locus_tag="IT904_003000"
     CDS             4606..4734
                     /gene="mntS"
                     /locus_tag="IT904_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAP3192747.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5006..6589
                     /locus_tag="IT904_003001"
     CDS             5006..6589
                     /locus_tag="IT904_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAP3192748.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6638..7153)
                     /gene="ompX"
                     /locus_tag="IT904_003002"
     CDS             complement(6638..7153)
                     /gene="ompX"
                     /locus_tag="IT904_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAP3192749.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7206..7271)
                     /locus_tag="IT904_003003"
     CDS             complement(7206..7271)
                     /locus_tag="IT904_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192750.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7505..8392
                     /gene="rhtA"
                     /locus_tag="IT904_003004"
     CDS             7505..8392
                     /gene="rhtA"
                     /locus_tag="IT904_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAP3192751.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8691..9194
                     /gene="dps"
                     /locus_tag="IT904_003005"
                     /gene_synonym="pexB"
     CDS             8691..9194
                     /gene="dps"
                     /locus_tag="IT904_003005"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAP3192752.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9598..10344
                     /gene="glnH"
                     /locus_tag="IT904_003006"
     CDS             9598..10344
                     /gene="glnH"
                     /locus_tag="IT904_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAP3192753.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10483..11142
                     /gene="glnP"
                     /locus_tag="IT904_003007"
     CDS             10483..11142
                     /gene="glnP"
                     /locus_tag="IT904_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAP3192754.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            11139..11861
                     /gene="glnQ"
                     /locus_tag="IT904_003008"
     CDS             11139..11861
                     /gene="glnQ"
                     /locus_tag="IT904_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAP3192755.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            12122..14347
                     /gene="ybiO"
                     /locus_tag="IT904_003009"
     CDS             12122..14347
                     /gene="ybiO"
                     /locus_tag="IT904_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAP3192756.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(14344..>15012)
                     /gene="rlmF"
                     /locus_tag="IT904_003010"
     CDS             complement(14344..>15012)
                     /gene="rlmF"
                     /locus_tag="IT904_003010"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAP3192757.1"
                     /translation="IHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRF
                     TGSETSSQALSSAQAIISSNPGLNRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPF
                     HDSAAAARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEESKGFAKQ
                     VMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFV
                     NRQR"
BASE COUNT         3656 a         3916 c         3672 g         3768 t
ORIGIN      
        1 tcatcagttt cagtagccct gaccatatca gggctactga taataactta ctcgatccct
       61 ttactacgca ggtaatcttc ataattaccg ctaaagtcga tcacgcgttc cggggtgatt
      121 tccagaatac gggtcgccag ggagcttaca aactcacggt cgtgagaaac aaagatcagc
      181 gtgccctgat acagttccag tgccatgttc agcgactcaa tggattccat atccaggtgg
      241 ttggtcggtt cgtccatgat cagaatgttc ggcttctgca tcattaactt accaaacagc
      301 atccgccctt tttccccacc ggaaagcact ttagctggct ttttgatgtc gtcctggctg
      361 aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt catcgccttc ctgcttccac
      421 tggctcatcc attcgaacac ggtcagatca ttttcaaact catattcgtg gtcctgagca
      481 tagtaaccaa tgcgcgcgtt ctcagaccat tttacggtgc cgctgtccgg ttgcagatcg
      541 cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag taccgccagt
      601 ttttcaccca cttccagcag caggttgaga tttttaaaca gcggaccgtt atcaaaccct
      661 ttggtcagac cttccacttc cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg
      721 atgaacgggt tctgacggct ggaggctttc acctcttcca gtttgatttt atcaatctgg
      781 cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa
      841 gattgcaact cagcaatctg cgctttcttc ttggcgttat cggccagcag acgttcacgc
      901 gcctgggtcg ccgccgtcat gtactcatcg tagttacccg gataaacgcg cagctcgccg
      961 taatccagat ccgccatgtg ggtacagacc atgttaagga agtgacggtc gtgcgagatg
     1021 atgatcatgg tgctgtcacg ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg
     1081 tcgaggttgt tggtcggttc gtcgagcagg agaatatccg gatcagcaaa cagcgcctgc
     1141 gccagaagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc gtagtgctgt
     1201 tccactggaa ttcccacgcc aagcaacagt tcaccggcgc gagcttccgc agagtaaccg
     1261 tccatttcgc cgtatttaac ttccagatcg gccactttat agccgtcttc ttcactcatt
     1321 tccggcaaag catagatgcg gtcgcgctcc tgcttcactt cccacaactc tttatgcccc
     1381 atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg acgcagttta
     1441 ccaatgcgct cgttgggatc gagggaaacg ttacccagcg tcggctcaag gtcgccgccg
     1501 aggatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc gtaacggttg
     1561 ccgccgccaa atttgacgga aatgttttca aacaacggct tactgccgaa ctgcatggtg
     1621 acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat
     1681 gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa gccaaaggca
     1741 aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact tataatgcgc
     1801 ttccaatact ctaatattct caacccaatg gcctgccagg cacaaaatct cgcttaacat
     1861 gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct tttgcagtac
     1921 cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg gtcagaatca
     1981 ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg cggagtacca
     2041 gatggggctt tccaatatga tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg
     2101 cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc atgaaggcat
     2161 cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca acaccgttat
     2221 cgtgctgccg atcggcattg gtcagttagg caaagatacg cctatcaact ggaccaccaa
     2281 agttgagcgt aagaaagcag gcccgacctg gacgccgacc gccaaaatgc acgcagagta
     2341 ccgcgctgcg ggcgaaccgc ttccggctgt cgttccggca ggtccggata acccgatggg
     2401 gctgtatgca ctctacatcg gtcgcctgta cgctatccat ggcaccaacg ccaacttcgg
     2461 tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct
     2521 gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg taaaagcgac
     2581 caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta ccaccgaagc
     2641 ccagtttgaa ggtcaggaaa ttgtgccaat taccctgacg aagagcgtgc aaacagtgac
     2701 cggtcagcca gatgttgacc aggttgttct tgatgaagcg attaaaaacc gctccgggat
     2761 gccggttcgt ctgaattaat cttcaaaact taaagcaaaa ggcggactca taatccgcct
     2821 tttttatttg ccagacccta gttggccggg agtataacta acaaaacata tcccaccaac
     2881 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
     2941 ttcgccatac gtagcgcaat caaatttgcc agcgatccgg gtaataaccc aaagccacct
     3001 acgtttaccg cccataccag taataaagac ggcggcacat agttcagcaa caatatggta
     3061 ctcggcacgt tactgatcac ctgcgataaa ccgattgccg ttaaccataa cccgggttca
     3121 gatagatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
     3181 acgtcgataa acatcgccat aaacaccagc agcagcgtcc agtccacact gagcaccacg
     3241 cgacgtgcca gcagcgcaaa gcctgccgcc acaatcacca gtccccacag ctcttgtttg
     3301 aactccagcg ccgtcagaaa gacgatatac agccccagac aactccacac cagccgcggt
     3361 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
     3421 cagcacagga gcaggagcgt cagcatcatt gcgccagcca gcggtgccat ttgggcaata
     3481 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
     3541 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
     3601 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
     3661 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
     3721 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
     3781 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
     3841 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
     3901 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
     3961 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
     4021 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
     4081 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
     4141 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
     4201 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
     4261 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
     4321 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
     4381 tcactttttt tgctgttggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
     4441 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
     4501 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
     4561 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
     4621 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
     4681 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttccgataa atagcggcgt
     4741 cgcggtacgc tgcttcactc ctgctttcat gcaggcataa cgcgttttgg tctgaaaaac
     4801 cccacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
     4861 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
     4921 acccctttcc actcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
     4981 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
     5041 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
     5101 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
     5161 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
     5221 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
     5281 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
     5341 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
     5401 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
     5461 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
     5521 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
     5581 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
     5641 taagccgaag tatcagacct atgtggtggt gatcggtgaa agcgcgcgtc gcgatgccct
     5701 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
     5761 atttgctgac tacattgccg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
     5821 tcgcgttgtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
     5881 gggcttccag acctggtggt tttccaacca gggtcaaatc ggcgaatacg ataccgctat
     5941 cgccagcatc gccaaacgag cagatgaagt gtacttcctg aaagaaggta attttgaagc
     6001 agataaaaac accaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
     6061 gcactcgcaa ccgcagctga ttgttctaca tctgatgggc tcacatccgc aggcctgcga
     6121 caggacacaa ggaaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
     6181 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
     6241 cagcagcttc tcgctggttt acttttctga ccacggtctg gcctttaaag agcgcggtaa
     6301 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
     6361 ggtcatttcc agcgacgata aagcgcatcg tgtgattaaa gcccgccgct cagccaatga
     6421 cttcttaggc tttttctccc agtggacggg gattaaagcg aaggaaatta acatcaaata
     6481 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
     6541 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
     6601 cgccccgaga ggcggatttt ttatatcacc aaagtgatta gaagcggtaa ccaacaccgg
     6661 caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag aagtccagag
     6721 caacgttttc catcgggttg aactgcagac ccgcaccgta ggagaaaccg tagtcgctgg
     6781 tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc acacccacta
     6841 caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg atgccgtagt
     6901 actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg taagtgaaag
     6961 aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg ttgaaaccgc
     7021 ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt acagtagaag
     7081 tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct gaaagacatg
     7141 caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt taaatatatc
     7201 aaaaattaat gggaaactct tcgcgatttg tgatgtctaa cgggccattt catgtaacag
     7261 aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag aactgcgtaa
     7321 gtattacgca tgttttccct gtcattcatc cagattattc ctaatcacca gactaatgat
     7381 tccatcaatc ctggcgcatt ttagtcaaaa cggggaaaat tttttcaaca aatgctcaac
     7441 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
     7501 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
     7561 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtggg
     7621 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
     7681 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
     7741 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
     7801 tgcggtggcg ctggagttca ccggaccact ggcggtggcg ctgttctctt ctcgtcgccc
     7861 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
     7921 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
     7981 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
     8041 ggcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
     8101 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
     8161 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
     8221 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
     8281 aacactgaca cccatacagc tactggcgct cggcgctatc atcgccgctt caatggggtc
     8341 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
     8401 ttctgcatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtctt
     8461 tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag cagataaata
     8521 agaattgttc ttatcaatat atctaactca ttgaatcttt attagttttg tttttcacgc
     8581 ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta
     8641 tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg
     8701 ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca
     8761 gcgagaaaaa agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt
     8821 ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg
     8881 aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg
     8941 cagtgcagct gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaaccccgc
     9001 tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagaa ctggctgacc
     9061 gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca
     9121 ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt
     9181 ctaacatcga ataaatccat cgctgatggt gcagaacttt agtacccgat aaaagcggct
     9241 tcctgacagg aggccgtttt gttttgcagc ccacctcaac gcacttattt agtgcatcca
     9301 tctgctatct ccagctgatt aagtaaattt tttgtatcca catcatcaca caatcgttac
     9361 ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt
     9421 ttagcgcacc agattggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg
     9481 tgcatcacgt ttttgccgca tctcgaaaaa tcaaggagtt gcaaaactgg cacgattttt
     9541 tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg
     9601 aagtctgtat taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat
     9661 gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa
     9721 cagggcgata aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagagctg
     9781 aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc
     9841 aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc
     9901 gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac
     9961 gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag cggtactggc
    10021 tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc
    10081 gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca cgatacgcca
    10141 aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct
    10201 ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa
    10261 gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa
    10321 tggttcggta ctgaaccgaa ataataacgc tacacctgta aaacgcactg gcagttccct
    10381 ctcccctatg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta
    10441 ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt
    10501 gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg
    10561 gtcctcggtc tggcaggcgg tctggtaatc ggattgctgg caggttttgc acgcaccttc
    10621 ggaggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg cggcacacct
    10681 atcgtcgtcc aggtgatgtt tatttatttc gccctgccga tggcgtttaa cgacttacgc
    10741 atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc gtatattgcg
    10801 gaaatcacgc gtggtgcggt gctgtctatc cacaaaggtt ttcgtgaagc aggactggcg
    10861 ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt
    10921 atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc gctgtttatt
    10981 gtgatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc
    11041 gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct ggtgctgagc
    11101 tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct
    11161 ccaagcactt tggcccaacc caggtgctgc acaatatcga tttgaacatt gcccagggcg
    11221 aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca
    11281 acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag gttaacgatc
    11341 cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc
    11401 tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg cccgctacgc gtgcgtggcg
    11461 cgaacaaaga agaggcggaa aaactggcac gtgagctgct ggcgaaagtc ggtctggcag
    11521 aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt gtggcgattg
    11581 cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact tccgctcttg
    11641 acccggaact gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa gaagggatga
    11701 cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cggctgatct
    11761 ttatcgacaa aggccggatt gcggaagatg gcaatccgca ggtgttgatc aagaacccgc
    11821 cgagccagcg cttgcaggaa tttttgcagc acgtctctta ataagacaca ttgcctgatg
    11881 cgctacgctt atcaggccta caggatatct ggcaacttat taaaattgca tgaacttgta
    11941 ggacggataa ggcgttcacg ccgcatccgg caaaaaagcc cgcacgttgt cagcaacctg
    12001 cttaatatcc cttcctccct ttcacccgaa agggaggcac accagattcc tctcatttaa
    12061 aatcgcccct cctccagcat ctatacttat ctttttgctc tattttctca ctggaggagt
    12121 catgcggtgg atcctgttca tcctcttctg cctgctgggc gcacctgccc acgcggtatc
    12181 catacccggc gttacaacca caacgacaac ggactcaacg actgaaccgg ccccggaacc
    12241 ggatatcgaa caaaaaaaag cggcctatgg cgcactggcg gatgtgctgg ataatgacac
    12301 ctcgcgtaaa gagttgatcg accagttgcg caccgttgcc gctacgcccc ctgctgaacc
    12361 ggtaccaaaa atagtgccgc cgacgctggt cgaagagcaa accgtgctgc aaaaggtcac
    12421 cgaagtcagc cgccattatg gtgaagccct ttccgcccgc ttcgggcaac tttatcgcaa
    12481 tatcaccggc tccccgcata agccgtttaa tccacaaacc ttcagcaatg cgctgaccca
    12541 tttttcaatg ttagcggtat tagtgtttgg tttttactgg ctgattcgcc tgtgcgcact
    12601 gccgctgtat cgcaaaatgg gtcagtgggc gcggcaaaaa aatcgtgagc gcagcaactg
    12661 gttgcagctt ccggcgatga ttatcggggc gtttattatc gacctgctgt tactggccct
    12721 gacattattt gtcggccagg tattaagcga caacctgaat gcaggcagtc gcaccatcgc
    12781 tttccaacaa agtttgtttc tcaacgcctt tgccctcatt gaatttttca aagccgtact
    12841 acgcctgatt ttttgcccaa acgtggcgga gctgcgcccg ttcacgattc aggacgagag
    12901 cgcccgttac tggagtcgtc gcctgagctg gttaagcagt ttgataggtt atggcctgat
    12961 tgtggccgtg ccgattatct ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt
    13021 catcattatg ctgtgcatga ccgtctgggc gttgtacctg atctttcgta ataaaaaaga
    13081 gattacccag catttgctca acttcgcgga gcattcgctg gcctttttca gcctgtttat
    13141 ccgcgccttt gcgctggtgt ggcactggct ggcaagcgcc tattttatcg tgctgttttt
    13201 cttttcgttg ttcgatccgg gcaacagcct gaaatttatg atgggtgcaa cggtgcgcag
    13261 cctggcgatt attggtatcg cagcgtttgt ttccggtatg ttttcccgct ggctggcgaa
    13321 aaccatcact ctctcgccac atactcagcg taactatccg gagctgcaaa aacggttgaa
    13381 tggctggctg tcggcggcgc tgaaaacggc gcgtattctg acagtctgcg tggcggtaat
    13441 gttgctgttg agcgcatggg gattgttcga tttctggaac tggctgcaaa acggcgcggg
    13501 gcagaaaacc gtagatatcc tgatccgtat cgcactcatt cttttcttct cggcggttgg
    13561 ctggacggtg ctcgccagtt tgatcgaaaa ccggctggct tcggatattc atggccgccc
    13621 gctacccagc gcccgcacgc gtaccctgct tacgctgttt cgtaacgcgc tggcggtgat
    13681 tatcagtacc atcaccatca tgattgtgtt gtcggaaatc ggcgtcaata tcgcgccatt
    13741 gctggcaggt gccggggcat taggtctggc tatctcgttt ggttcgcaaa cgctggtgaa
    13801 agatattatc accggggtat ttattcagtt tgaaaacggc atgaacactg gagatttggt
    13861 gactatcggg ccgttgaccg gcacagtgga acggatgtcg attcgctccg tgggcgtacg
    13921 ccaggatacc ggggcgtatc acatcattcc gtggtcttcg ataaccacct ttgctaactt
    13981 tgtccgcggc attggttcgg tggtggcaaa ttatgatgtt gatcgccatg aagatgctga
    14041 taaagccaat caggcactga aagatgcggt agcggaatta atggaaaacg aagaaattcg
    14101 cgggctgatt attggtgaac cgaattttgc cgggattgtc ggcttaagca ataccgcgtt
    14161 tacactgcgt gtttcgttca ccacgctgcc actcaaacag tggacggtac gctttgccct
    14221 cgacagccag gtgaaaaaac atttcgacct ggcgggcgtt cgcgcgccag tgcagactta
    14281 tcaggtgctg cctgctccgg gcgcgacccc ggctgaaccg ttaccgccgg gggaaccaac
    14341 gctttaacgc tggcgattga caaaacggcg gcgctgctcg tcgttcataa aggtccaggc
    14401 aataaagcga ctctgctttt gcccctgggc catctctttt ttaaccacct tcaccgcgcc
    14461 cacgtccgtc agggcacgat acaacggcgg taagttttca ccacgagata ccagtgatgt
    14521 aaaccacatc acctgcttcg cgaagccttt gctctcttca atcatctttt tgataaaggt
    14581 gacttcaccg ccttcacacc acaactcctg ttgctggccg ccaaagttca gtgcatcgtc
    14641 tttgttcagc cccaggttac ggcgtttacg ctcactacct gcccgtgccg cagcggcgga
    14701 atcgtggaat ggcgggttac acaaggtcgc gtcgtattgc tcgtttttat ggatgatgcc
    14761 gttaaaaatc gccccactct ctttttgccg acgcagacga atggcgcggt taagccccgg
    14821 attagaactg ataatcgcct gcgcactgct taacgcctgg ctgctggttt cactaccggt
    14881 aaaacgccag ccatattcat gtacgccaat cagcggataa atacagttcg caccaacgcc
    14941 gatatccaga atgctggcat tcgctggaat tgttccgctt gcttctgcca gtaaatcggc
    15001 aaggtgatga at
//
LOCUS       DACAPB010000111        15001 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.111, whole genome shotgun sequence.
ACCESSION   DACAPB010000111 DACAPB010000000
VERSION     DACAPB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.111"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1163
                     /locus_tag="IT904_003011"
     CDS             <1..1163
                     /locus_tag="IT904_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAP3192758.1"
                     /translation="DANHLSVNDDKDTIRTYSRMKNQFTVSVNQPLSFAYEDYGSLFL
                     SGSWTNYWAGNNNRTEYNVGYSKSVSWGNFSVNLQRSWNEDGNKDDAMYLNVSVPLEN
                     IFGGKRKSSGFRNLSTQFNTDFNGSHQLNVSSSGNSEDNLIGYSVNTGYNLDKESENV
                     ASVGGYLSYDSTWGGFSASASASTDNRQQYSVSTDGGFVLHSGGLTFTNNSFGSNDTL
                     VVIKAPGAKGARVNNGTDEIDRWGYAVSSSLSPYRENRVGLNIETLENDVELKSTSAT
                     TVPRSGSIILASFETDQGRSAVLNISTSNGKPIPFAAEVYQDEIMIGSMGQGGQAFVR
                     GINDSGELIIRWFENSRTINCKLHYQLPAQPETLGSTNTLLLNNLTCKLVNH"
     gene            1177..1734
                     /locus_tag="IT904_003012"
     CDS             1177..1734
                     /locus_tag="IT904_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192759.1"
                     /translation="MKRIIIISTLVGLGLPTTGSATNINVDFTAQLLATTCTITIKED
                     GGPAVNNTGGDDYSMTIPTVGLDKIANAHADAQANFQLVAKDCSEGYSKIITKLSGAT
                     ISGNLIKNEATDTPVATNIGMGIKRRDAADASFITPNNSASVEWTTEDKTNGMKLTVA
                     LRETTAGTGNIGNFRAKATFNFTYE"
     gene            1749..2345
                     /locus_tag="IT904_003013"
     CDS             1749..2345
                     /locus_tag="IT904_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192760.1"
                     /translation="MKLKAIAILIVTGGMISHSALAGTTSATLTVDSIVTMGTCTAQL
                     LDSNDKEISQVAIGDVYISELRNETNKKAFKIRSSGCAGLPQKKALVKLTPRGIGCGG
                     YNANSAGFANAKNGAGAAGRVAVEVWTTKNAEIEAGNEIFNCFSPAEKTVNLSSVTAT
                     QHFDYDLSAKMVVAEGRQLSDVTPGEFLSPATFTISYQ"
     gene            2366..2956
                     /locus_tag="IT904_003014"
     CDS             2366..2956
                     /locus_tag="IT904_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192761.1"
                     /translation="MLFQEHNFALFFGLVTALCSPVSLANKDVDLTANIVSSTCQIEI
                     NNNGVVDLGTVTLDYFSNNITPDTDYAGGKNFTVNVVSCDNLQTTQSQIKFDFQPQTG
                     SLAQANQQVFSNQYELQSIGAKNVGVVIFSAQPHAQKFNVRGTDGASKAIYALPSNQA
                     VPSTWTFYARMQKVNNALAPVAGIVRSQVIVNVYYE"
     gene            2968..4081
                     /locus_tag="IT904_003015"
                     /pseudo
     CDS             2968..4081
                     /locus_tag="IT904_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4215..5009
                     /gene="panB"
                     /locus_tag="IT904_003016"
     CDS             4215..5009
                     /gene="panB"
                     /locus_tag="IT904_003016"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP3192762.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            5021..5872
                     /gene="panC"
                     /locus_tag="IT904_003017"
     CDS             5021..5872
                     /gene="panC"
                     /locus_tag="IT904_003017"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP3192763.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPDLYKVLSSIADKLQVG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(5946..6848)
                     /locus_tag="IT904_003018"
     CDS             complement(5946..6848)
                     /locus_tag="IT904_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP3192764.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            7122..7502
                     /gene="panD"
                     /locus_tag="IT904_003019"
     CDS             7122..7502
                     /gene="panD"
                     /locus_tag="IT904_003019"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP3192765.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(7506..8735)
                     /locus_tag="IT904_003020"
     CDS             complement(7506..8735)
                     /locus_tag="IT904_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP3192766.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKIEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDNAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(8799..9239)
                     /locus_tag="IT904_003021"
     CDS             complement(8799..9239)
                     /locus_tag="IT904_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP3192767.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(9344..10114)
                     /locus_tag="IT904_003022"
     CDS             complement(9344..10114)
                     /locus_tag="IT904_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3192768.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(10111..11037)
                     /locus_tag="IT904_003023"
     CDS             complement(10111..11037)
                     /locus_tag="IT904_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3192769.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            11146..11808
                     /gene="can"
                     /locus_tag="IT904_003024"
     CDS             11146..11808
                     /gene="can"
                     /locus_tag="IT904_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAP3192770.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(11849..12385)
                     /gene="hpt"
                     /locus_tag="IT904_003025"
     CDS             complement(11849..12385)
                     /gene="hpt"
                     /locus_tag="IT904_003025"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP3192771.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            12591..14981
                     /locus_tag="IT904_003026"
     CDS             12591..14981
                     /locus_tag="IT904_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAP3192772.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWLSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
BASE COUNT         3976 a         3741 c         3541 g         3743 t
ORIGIN      
        1 acgacgccaa tcatctgtca gtcaacgacg acaaagacac gatccgtacc tattcacgca
       61 tgaaaaacca atttaccgtt agtgtaaatc agccgttgag ttttgcttat gaggattacg
      121 gttcactctt tttatctggc agctggacaa actactgggc tggtaacaat aaccgaactg
      181 aatacaatgt tggttacagt aaaagtgtct cctgggggaa tttcagcgtc aacttgcaac
      241 gtagctggaa tgaggacggc aataaagatg atgcgatgta tttgaacgtt agcgttccgc
      301 tggaaaatat tttcggcggt aaacgtaaat cctcagggtt ccgtaaccta agcactcaat
      361 tcaatactga tttcaacggt tcgcatcaat taaacgtcag tagttcgggt aacagcgaag
      421 ataatctcat cggttacagt gtgaatacgg gttataacct tgataaagaa tctgaaaatg
      481 tcgcttccgt tggtggatat cttagctatg actccacatg gggaggtttt tccgcttctg
      541 cttctgctag caccgataac agacaacaat actctgtctc taccgatggt ggttttgtac
      601 ttcacagtgg cggcctgacc tttaccaata acagtttcgg tagcaatgac actttagtcg
      661 tcattaaagc acccggagcg aagggggcac gagtcaataa cggtactgat gaaatcgatc
      721 gctggggtta tgccgtttcc tcatccttaa gtccataccg tgaaaaccgg gtgggattaa
      781 atattgaaac attggaaaac gatgtcgaac tgaaaagtac tagtgccacc actgtacctc
      841 gtagtggttc cattattctt gccagttttg aaactgacca ggggcgttct gcagttctga
      901 atatcagcac cagtaacggt aaacctattc cttttgccgc tgaagtttat caggatgaaa
      961 ttatgatcgg cagtatgggt cagggtggtc aggcatttgt acgcggtatt aatgacagcg
     1021 gagagttaat tatccgctgg ttcgaaaaca gtcgaacaat aaattgcaaa ttgcattatc
     1081 aacttcctgc acagccagaa acacttggaa gtacaaacac cttattatta aacaacctta
     1141 cctgtaagtt ggttaaccac taaaatggaa cattctatga aaaggatcat aataatatct
     1201 acgctagttg gccttgggtt acccacaact ggctcagcga caaatattaa tgtcgatttt
     1261 accgcacaat tgctggcgac tacttgtaca ataacgatta aagaagatgg aggccccgcg
     1321 gttaataata cgggcggtga tgattatagt atgactatcc caacggttgg gctggataaa
     1381 attgccaatg ctcatgctga tgctcaagcc aattttcaat tggtggccaa agattgtagt
     1441 gagggttatt cgaaaattat cactaaactt tctggagcaa ccatttccgg taacttgatt
     1501 aaaaacgaag cgacggacac tcctgtagca accaatattg gtatgggcat caagcgccgg
     1561 gatgcagctg atgcttcgtt tattacgcct aataatagtg caagtgttga gtggactacg
     1621 gaagataaaa ctaatggaat gaagttaacc gttgccctgc gcgaaactac agcagggaca
     1681 ggaaatatcg gtaatttccg tgcgaaagcc acctttaact tcacctacga atgacaggaa
     1741 cagcgaccat gaaattaaaa gcaatagcaa tactgattgt tactggtgga atgattagcc
     1801 atagtgccct ggcaggaaca acgtctgcaa ccttaacggt agacagcatt gtcacaatgg
     1861 gtacttgtac tgctcaatta ctggactcca atgataaaga aatctcacaa gttgcgattg
     1921 gtgacgttta tatttctgaa ctaagaaatg aaaccaataa aaaagccttt aaaatccgtt
     1981 ctagtggttg tgccggactt ccgcaaaaaa aagcactggt aaaattaact cccagaggaa
     2041 taggttgcgg tggttataat gctaactcag ccggttttgc aaatgcaaag aatggtgcgg
     2101 gcgcagcagg aagagttgca gttgaagtat ggactaccaa aaatgcagaa attgaagctg
     2161 gcaatgaaat ttttaattgt tttagccccg cagaaaaaac ggttaacctt tcttccgtca
     2221 ctgcgacgca acatttcgat tatgatttaa gtgctaagat ggttgttgct gaaggcagac
     2281 agttgagtga tgtcacaccg ggtgagtttc tttctccagc tacctttaca atctcttatc
     2341 agtaacaaca gataaggaaa ttataatgct tttccaggaa cataatttcg cacttttttt
     2401 cgggctggta acagccctct gctctcctgt tagcctggct aataaagatg tagatcttac
     2461 tgcgaacatt gtgtcaagca catgtcagat cgaaataaat aataatggtg tagttgatct
     2521 cggcaccgtg acactggact atttttctaa caatataacc ccagatacgg attacgctgg
     2581 cggtaagaat tttactgtca acgtcgtttc ttgtgataat ttgcaaacca cacaatcgca
     2641 gattaaattc gattttcaac cacaaactgg atctcttgcc caagcaaatc aacaagtttt
     2701 cagtaaccaa tacgagttac aatctatcgg agctaaaaat gttggggttg ttattttttc
     2761 agcacagccc catgcacaaa aatttaacgt tcgggggacg gacggagcat caaaagccat
     2821 ttatgcctta ccctctaatc aagcggttcc gtcaacgtgg acattctatg cccgaatgca
     2881 aaaagtcaat aacgctcttg cacctgttgc tggcattgtg agaagtcagg ttatagttaa
     2941 cgtttattac gaataataag gattactatg caagggaaag tcaaaaaaat tttgcttcat
     3001 atatacagtg cagtgctatt atttttggca tcgtcatggt atgtttttgc tggcacaggt
     3061 tctgaatgct atatagaaag cagttcggcc gggagttata ccattttcat taatacccct
     3121 ccctatagtg tagcgacaca gcaggtcact actccttacg aaatatctga tgcagccaca
     3181 gatgtaccaa ttgtacttcg gggtgatgga attgcatgta aagccatacc aaatggcgat
     3241 gagagtatac attttcttaa taccgctgat gccaatctaa tatcaacata tacttcagca
     3301 cgaggttcta cattattaaa aaccaccgtt ccgggtattg cttatactgt tgaattaatt
     3361 tgtgatacct gtcctgaagt agtcgactta cgcattccac cagctggcga cgataacttc
     3421 acccccaact ccgatatctg gtgggtgtgg ggcgaaacag atgaaaaatg gaagttacgt
     3481 tttcgctttt tctatactcc ggagtttaaa cctaaaaatg gtgttaccag tggaacgtta
     3541 ttaccgggaa aaattgcttc ctggtatatt ggtatcaaca cccaaccatg gatcaacttt
     3601 tacgttgatg cagatacatt taaatttacc gtggatcaac ctacatgtag taccattgct
     3661 ctggattcaa ccagccaaaa tatcagcggt aataaaataa acttgggaga ctattatgtt
     3721 tcccaggtta agcaggaaat cacccaagta gttccattta ctattcgggg cgattactgt
     3781 tacgcaagca aaattacagt gaagttgaaa gccacaactg aggcaccaaa taaaaaaatt
     3841 aattggaaaa tcatccggct cagcaaccgg tgttggtgta aaagtctact caactgcgaa
     3901 caacagtgag gttcagctaa acgcagacaa cagcaacgag attatattta actacgctaa
     3961 ctggtcaaac aaccttcttt attttccgtt tacggcgcaa ctggttaaag atggaagtaa
     4021 tggcacaatt agccctgggg attttaccgg taatgcgaca ttctcttttt cctatgaata
     4081 agtaagtctc accccaactc aacgcaccag attcaaactc tggtgcgaaa ctttcttcac
     4141 actaaaaacc attgcccccc tgatttcgac tgttagaatg caatcagatc tgactttagc
     4201 tatcaggata cgttatgaaa ccaaccacca tctccttact gcagaagtac aaacaggaaa
     4261 aaaaacgttt cgcgaccatc accgcttatg actatagctt cgccaaactc tttgctgatg
     4321 aaggacttaa cgtcatgctg gtaggcgatt cgctgggcat gacggttcag ggacacgact
     4381 ccaccctgcc agttaccgtt gccgatatcg cctaccacac tgccgccgta cgtcgcggcg
     4441 caccaaactg cctgctgttg gctgacctgc cgtttatggc gtatgccaca ccggaacaag
     4501 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa catggtcaaa attgaaggcg
     4561 gtgagtggct ggtagaaacc gtacaaatgc tgaccgaacg tgccgttcct gtatgtggtc
     4621 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
     4681 gcgatgaagc gggtgatcaa ctgctcagcg atgcattagc cttagaagcc gctggggcac
     4741 agttgctggt gctggaatgc gtgccggttg aactggcaaa acgtattacc gaagcactgg
     4801 cgatcccggt tattggcatt ggagcaggca acgtcactga cgggcagatc ctcgtgatgc
     4861 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     4921 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     4981 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     5041 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     5101 gcctaccatg ggtaacctgc acgatggcca tatgaagctg gtcgacgaag ccaaagcccg
     5161 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     5221 tctggctcgt tatccacgca ccttgcagga ggactgcgaa aagctaaaca aacgtaaagt
     5281 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     5341 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     5401 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     5461 cttcggtgaa aaagattttc agcaactggc gctgatccgc aaaatggttg ctgatatggg
     5521 ctttgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctgag
     5581 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcccgatc tgtacaaagt
     5641 tttaagttcg attgccgaca aattgcaggt tggcgaacgg gatctcgatg aaattattac
     5701 tattgccggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     5761 tgccgacacg ttgctggaag tctctgaaac cagcaaacgg gctgtgattc tggtagccgc
     5821 ctggcttggc gatgcccgcc tgatcgacaa caaaatagtc gaactggcgt aatacttaac
     5881 tggcgctacg gctgatggcg ccagttatta atttgcccta cgtcatctgt cataacacag
     5941 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     6001 gcaacgtctt cccgagacat tcctttactc agaagacgca gggcgaattc ttgcctttcc
     6061 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     6121 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     6181 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     6241 gtgtaccctt cgtcaatcag cgtgaccagt tgctcaagca ataacattaa gtcgcgctgg
     6301 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     6361 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     6421 tccggcgagt aaaacatatc aaaccagcac attgatagcg gataaggtgt ggcctcgccc
     6481 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggtcagcctc cagatgccgg
     6541 tgcatggcgg ctatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     6601 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcac ggaatagagc
     6661 acgtccgtgc tgtgtccttt caggctctct tcaatgaaac tccccgactc taaatgaagc
     6721 gtgttgaggt cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     6781 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     6841 gcatccatct tccctgtctt ccgtgtggct ttgcgtgaag gcagtatcca gtcaaggcgc
     6901 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcagagaaat atcaattcgt
     6961 tacaggcgat acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccgtca
     7021 gatgagtaga cactaaacaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     7081 atcgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     7141 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     7201 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc attgatatct
     7261 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     7321 gaattatttc tgttaacggt gcggcggccc actgcgccag tgtcggcgat attgtcatca
     7381 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aacgtcgcct
     7441 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     7501 gattgttatc cctgcggctg gttactcacc agccgcgaca tcgtctccag cgaatccgtt
     7561 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     7621 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc gttgtcatta
     7681 aatccgccaa acggatacga aagataccag acatgcggat taaattgcgc cagagcgcgg
     7741 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     7801 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa tacatcgcga
     7861 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     7921 tgacgtttga tgcgtgaggt aacaataaac gccgtcgcct tcatgccata ttgtttcaac
     7981 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     8041 cgcgcaggga gattgatctt attcttcacg tagccttcca gctgcaccat gctcagtgtc
     8101 gcgtatcccc tgtcacgcag ccaggccatc tggttattga aagcgcgtac cgatgtggtc
     8161 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataggttagc
     8221 accgacaggc cattatcggg ttgggcatcc agtgcgctga tataggccag tcgatcgcca
     8281 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     8341 cgcaaattgt ccgccagtac cccaaatggc gcacttcccg cgctcggcgc gttatagacc
     8401 ggcgtatctt tccaggtaac taagttctga ttactcagcg gcttgttgag gtcgcccaaa
     8461 ccgtcttcaa ttttttgtcg cccctgaacc ggctcgagat gacctttatc gataaaacct
     8521 ttgccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     8581 tgtccggccc gaatattccc cacggtcacc atcttgtcac caatttgcgc ccagaccgca
     8641 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     8701 agcatcagca ggagaataac agcttgtttg tacatgaaaa aataaccgag ggtgaaacaa
     8761 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     8821 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     8881 cccagttcga caatacgctc gcgaaactct gaactggtca tggtttcacg gcaagccatc
     8941 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     9001 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     9061 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagaa
     9121 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gcgccccatg ttttttctcc
     9181 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     9241 gccttcctcc ttagccgaaa atcagtctac cctgaccatt gttgataaag gatgatagcc
     9301 gtcaaaactc ctctccccta aatccagagg agaggaaata gccttagcta cgcaaaccac
     9361 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     9421 cgccaaaggt agtgaccagc ggaacatcat tgataccgag gaagccgtag cggaaaccac
     9481 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     9541 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     9601 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaaca
     9661 acaccgccgt aagcaccagc gttaaagcga caaataccca cgaatgcacc tgaaacggca
     9721 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     9781 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     9841 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     9901 tcaccgacat catgatcagc cccggtacga tgaactgcat gtagctgaag ccatgcatat
     9961 cgccaatacg cgaaccaatc aggttaccga agataataaa gtaaagggtc atggtgatga
    10021 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
    10081 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
    10141 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
    10201 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgca gcacttcaac
    10261 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
    10321 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
    10381 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
    10441 tgcttcttcc aggtagtgtg tggtgagaat gatggtggtg cctttgtcgt ttaaatcctt
    10501 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
    10561 aatcagtagt ttaggctcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
    10621 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
    10681 cttttcgctg cggatgtacg cttctttgcg ctccacaccg tagtaccctg cctgattaac
    10741 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
    10801 ctgacgttta gcgttcacga catccttctc gagatcgtaa ccaaatacgc tgacccgccc
    10861 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
    10921 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
    10981 ctgaacgccg cctggatagg tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
    11041 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
    11101 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
    11161 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
    11221 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
    11281 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
    11341 gttgctaacc tggtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
    11401 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
    11461 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
    11521 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
    11581 ttgtgtgaac tgaacgtcat ggaacaggtt tataacctgg gccactccac cattatgcaa
    11641 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctatgg cattcacgac
    11701 ggcttgctgc gtgatctgga tgtcacagcg actaaccgcg aaacccttga gcaacgttac
    11761 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
    11821 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccgatat
    11881 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
    11941 ggatcgagaa accgataaat tctaccggga cgttcacttc acgacgggac ggtttatcca
    12001 gcagcgtaca aatcgccagc gacttcggtt cgcgcaggct taagatctca cgcactttcg
    12061 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
    12121 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
    12181 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
    12241 taaacataaa tgagccacgc agcagaccaa ccagcaccat atcgctgccg ctgtctttgt
    12301 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
    12361 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
    12421 gcacgggcag gttttggcac cgcaacaaag cgcaagacac gcagtatacc agtaaaacta
    12481 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acgatcacac atgttaaaac
    12541 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
    12601 acaatacagg ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
    12661 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
    12721 ctatcgctgg tcttgtgatg ctcggcgtcg cctggatgct gtggctcagt aaacgtgccg
    12781 cgctttggct gtacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
    12841 gtttcgactt ctgggcgctg actccgcgca gcgacattct ggtcttcttc ggcatctggc
    12901 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
    12961 tggtggtggc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
    13021 aggaaatcaa cggcacctta agcgccgatg ccacacctgc ggaagctatc tcacccgttg
    13081 ccgatcagga ctggcctgcc tatggtcgca atcaggaagg tcaacgcttt tcacctctga
    13141 aacaaattaa cgccgataac gttcacaagc tgaaagaagc ctgggtgttc cgtactggcg
    13201 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
    13261 gcgacaccct ttacctgtgt accgctcacc agcgactgtt tgcgctcgat gccgccagcg
    13321 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
    13381 cctgccgtgg tgtctcttat catgaagcca aagcagaaac cgcttcgccg gaagtgatgg
    13441 cggattgccc gcgtcgtatc attcttccgg tcaatgatgg tcgccttatt gcgattaacg
    13501 ctgaaaacgg caagctgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
    13561 atatgccaga caccaaaccg ggtctgtatg agccaacttc gccgccgatt atcaccgata
    13621 aaaccatcgt gatggcgggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
    13681 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat cccggcgcga
    13741 aagatccaaa cgcaatcccg tctgacgaac acacctttac ctttaactcg ccaaactcgt
    13801 gggcaccagc ggcctatgac gcgaagctgg atctggtgta tctgccgatg ggcgtgacca
    13861 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    13921 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    13981 tgtgggatat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    14041 aagtgccagt tatttacgct ccggcgaaaa ccggcaacat ttttgtgctc gatcgtcgta
    14101 atggcgaact ggtggttccg gcaccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    14161 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    14221 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    14281 accagatgcg ctatgaaggc attttcaccc cgccatctga acagggtacg ctggtcttcc
    14341 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    14401 cgattgccaa cccaatggca ctgccgtttg tttcgaaact gatcccgcgt ggtcctggca
    14461 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    14521 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    14581 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    14641 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    14701 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    14761 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    14821 tgtggcaggg tcgtttacca gcgggtggtc aggctacgcc aatgacctat gaagtgaatg
    14881 gtaagcagta tgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    14941 actatattgt ggcttatgcg ctgccggatg atgtgaagta agacttgctc agattgctga
    15001 c
//
LOCUS       DACAPB010000112        14965 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.112, whole genome shotgun sequence.
ACCESSION   DACAPB010000112 DACAPB010000000
VERSION     DACAPB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.112"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..320
                     /locus_tag="IT904_003027"
     CDS             <1..320
                     /locus_tag="IT904_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP3192773.1"
                     /translation="KAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLTVVAREGTP
                     HVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETV
                     ERM"
     gene            497..985
                     /locus_tag="IT904_003028"
     CDS             497..985
                     /locus_tag="IT904_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAP3192774.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSDDDS
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(1105..1497)
                     /locus_tag="IT904_003029"
     CDS             complement(1105..1497)
                     /locus_tag="IT904_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAP3192775.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(1497..3575)
                     /gene="flhA"
                     /locus_tag="IT904_003030"
                     /pseudo
     CDS             complement(1497..3575)
                     /gene="flhA"
                     /locus_tag="IT904_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(3568..4716)
                     /gene="flhB"
                     /locus_tag="IT904_003031"
     CDS             complement(3568..4716)
                     /gene="flhB"
                     /locus_tag="IT904_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAP3192776.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(4918..5562)
                     /gene="cheZ"
                     /locus_tag="IT904_003032"
     CDS             complement(4918..5562)
                     /gene="cheZ"
                     /locus_tag="IT904_003032"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAP3192777.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(5573..5962)
                     /gene="cheY"
                     /locus_tag="IT904_003033"
     CDS             complement(5573..5962)
                     /gene="cheY"
                     /locus_tag="IT904_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAP3192778.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(5977..7026)
                     /locus_tag="IT904_003034"
     CDS             complement(5977..7026)
                     /locus_tag="IT904_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAP3192779.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIASGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(7029..7889)
                     /gene="cheR"
                     /locus_tag="IT904_003035"
     CDS             complement(7029..7889)
                     /gene="cheR"
                     /locus_tag="IT904_003035"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAP3192780.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(7908..9509)
                     /gene="tap"
                     /locus_tag="IT904_003036"
     CDS             complement(7908..9509)
                     /gene="tap"
                     /locus_tag="IT904_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAP3192781.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            complement(9555..11216)
                     /gene="tar"
                     /locus_tag="IT904_003037"
     CDS             complement(9555..11216)
                     /gene="tar"
                     /locus_tag="IT904_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAP3192782.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(11361..11864)
                     /gene="cheW"
                     /locus_tag="IT904_003038"
     CDS             complement(11361..11864)
                     /gene="cheW"
                     /locus_tag="IT904_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAP3192783.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(11885..13849)
                     /gene="cheA"
                     /locus_tag="IT904_003039"
     CDS             complement(11885..13849)
                     /gene="cheA"
                     /locus_tag="IT904_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAP3192784.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTKLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(13854..14780)
                     /locus_tag="IT904_003040"
     CDS             complement(13854..14780)
                     /locus_tag="IT904_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein MotB"
                     /protein_id="HAP3192785.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(14777..>14965)
                     /locus_tag="IT904_003041"
     CDS             complement(14777..>14965)
                     /locus_tag="IT904_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP3192786.1"
                     /translation="TSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIELE
                     EHVRAVKNPQQQTTTEEA"
BASE COUNT         3465 a         4217 c         3664 g         3619 t
ORIGIN      
        1 gtaaagcaga aattgacgaa gagcaactgg ctgcagctcc ggtaatcatc cgtgaagatc
       61 gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc gtggttgccc
      121 gtgaaggtac gccgcatgtg atgtgtgctt acctgtacga tctggccggt ctgttctctg
      181 gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt aacagccgtc
      241 tgaaactggc acaactgacg gcgaagacgc tgaagctggg tctggatacg ctgggtattg
      301 agactgtaga gcgtatgtaa tcgatttttc gtgagagtga agcctgatca gggttacctg
      361 atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca tcgttttctc
      421 actgttgggt aagaagtcag aagcgtaata ttcggcccca gggaaacgat aacggttgaa
      481 tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggtatta tctcttgcca
      541 ggtttacgca gaagatacgc ttatagtaaa cgaccatgac atttcagcca tcaaagattg
      601 ttggcaaaaa aattcagatg atgatagtga cattaacgtg atcaaatcat gcctgcgaca
      661 agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt atcgttatat
      721 agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta acttgcttaa
      781 aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa tcctttcaaa
      841 tgaggacgtt caggatttaa gtgaccctta ttctgaatca gaatggctct catgtatgat
      901 catacagacc aatacgcgaa ctcgccagtt gcagctatac cgtaactctg aagattttta
      961 tccaagccct ttgacaagag gataattcac atatttttgg catgttttgt tgcaagctat
     1021 tcctgataaa taattgcaac aaaacatcga gcctttttca ctgagttatt aaacatactc
     1081 gcgagcgcgt aatttttttg tccttcagcg ataattcaca atcacttcat tacgttgtac
     1141 tttcagcggc ggaattaacc gcccaccgcc aggcacttcc cagataaagc gcaacggttc
     1201 tgctgccgct ataccggaaa aggccacggt ggttccgctc tgcccctcta attcgacaca
     1261 acgagattgc gaacacaagc gaacccgcag tcctgaaggt gtcgggccga taagctgata
     1321 acgccacgct accagcgtca tcaatcctga agcaggttgt cgcgtagaaa gaggcgcaga
     1381 cgacatcgac tcaccgcgat gatttagcgt aattcctaca ctacttgcct gccacatccc
     1441 ctccccggcg gcttgcacca gcagcggaaa caataatatt gctaataagg ttctcattat
     1501 ttgccgccaa ttgtcgccgt catgcggata tgtcggttat cagacagttc cagattcgac
     1561 aggaccacta actgcggcaa gctgcggcgc aggaagcgag acaataatgg tcgcagcgcg
     1621 tggttcacca acaatactgg cggcgcaccc agcatctcct gacgggatag cgcttcctga
     1681 gtttgcgcca gtaaacgatc cgccagccct ggctccagtc ctcccccgcc ctgcagcgcc
     1741 tgtagtaaca aacgttccag cggtgtatcg aggccaataa catggacttc atctttgcca
     1801 ggaaaccact gctgggtaat cgcccgtccc aacgccacgc gcacgacggc ggttaattca
     1861 tgtggatcgc tttggatggg cgcatgttcc gccagcgttt cgagaatggt gcgcatatcg
     1921 cgaatcggta ctttttcatc gaggagattt tgcagcactt tatgcagtgt ggtgagcgtg
     1981 acgacgccag gaacgagatc ttccgtcagc tttggcatct cctgggcgac gcgatccaac
     2041 agctgttgcg cctcctgacg accaaacagc tctgcggcat gctggctaat gaggtggtta
     2101 agatgcgttg ctaccaccgt gctggcctca accactgtgt acccctgaat ctacgcctgt
     2161 tcttttagcg cactttcaat ccagatagca ttcaggccaa atgccggatc gacggtcgcc
     2221 tcaccaggta acgtcccggc agcgattcca gggttaatcg ccagccagcg ccccggataa
     2281 gcatcaccac tgccaatctc cacgcctttc atcaaaatgc gatagcgggc aggttgcaga
     2341 tccatattgt cgcgaatgtg caccactggc ggcagcaatc ccatctcctg ggcaaatttc
     2401 ttgcggatac tgcgtatacg gcccaacaac tcaccatcct gctggaaatc gaccatcggg
     2461 atcagtcgat aacccacttc cattcccaga gaatcttcca gttgtacatc gttccacgtc
     2521 gcttcgacaa cggtattatt ctctgccatt tttaccggtt tgggttcggc aggcgctttt
     2581 tgttcgcgtc cgcgtatcca ccaggccagc ccgagcaatc cggcagtgaa cagcaaaaat
     2641 accaggttcg gcattccagg caccaggccg agtaaaccga gcacggcggc gcttaacaac
     2701 ataacgcttg ggttactgaa aagctgattc accatctgct cgccaacatc ctgatcggtg
     2761 ctgacacgcg taacgatgac ccccgcggcg gtagaaatca ccagcgccgg aatttgtgcc
     2821 accagaccgt cgccaatggt caatagcgta taactttccg ccgcgtgtcc catgctcatg
     2881 ccatgttgca gcacgccgac cagcaacccg ccgacaacgt taatgaccat gatgaggatc
     2941 ccggcgatgg catcgccgcg aacaaactta cttgccccgt ccattgagcc gtaaaaatcg
     3001 gcttcctgag tcacttcgga gcggcgtttt ttcgcctcat cttcaccaat caatccggcg
     3061 ttaaggtcgg cgtcaatcgc catctgctta cccggcatac catcgagaac aaagcgcgca
     3121 cccacttctg cgatacgccc ggcacctttg gtaatgacca taaagttgat gatcacgaga
     3181 atgacaaaca ccacgatacc gatagcgaaa ttgccaccaa cgaggaagtg accgaacgct
     3241 tcgaccacct tccctgccgc cgccgcgccg gtatgccctt ccattaaaat gatacgggtt
     3301 gaagccacgt taagtgccag acgcaacagc gtggtaaaca acagaatggt cggaaacgca
     3361 gcaaactcaa gcgtgcgctg ggtaaacatc gccaccagca acaccatgat cgacaaggca
     3421 atattgaagg taaacaacag gtcgagtatg aatgcgggca gtggcagcac catcatcgac
     3481 aagatcaaca ggatcaaaat cggtccggca aggatctgcc attgtgtcga tttcaggttt
     3541 gcgggcaggc gcagcatcgc ggccagatta ctcatgggtc ggtttctcgt taataaaatc
     3601 cagggcttcc ggcaccggaa gatgagtagg ttgtacaggg cgctgtccac cagccagacg
     3661 ccagcgtttc agttgccaga cccaggccag cacttccgcc accgcggcgt acagttgacc
     3721 cgggatttgt tgaccaatct ccgcatgtcg atacagcgct cgcgccagcg gcggcgcttc
     3781 aagcgtcggg acgttatttt cagcgccaat ttcacgaatg cgcagcgcga ccagccctgc
     3841 acctttagcg accactttcg gtgcgctcat tttgttttcg tcatactgca acgctaccga
     3901 atagtgggtc gggttattga caatgacatc cgctttcggc acatcggcca tcatccgacg
     3961 ccgtgcggca gctcgctgca tctgacggat ccgtccttta acatgggggt cgccttcgct
     4021 ttgtttgaac tcatcacgaa tatcctgccg cgacattcgc agctttttca ggtggctgaa
     4081 gatttggaaa aagacgtcaa atcccaccat tggaatgaca ccaagcacca ccagcagtgc
     4141 gcatagccct accaaatcca tcgcattacc catggcggta atcggagact cggccattaa
     4201 gcgcatcatc tgcggccagt gatgccagag aaaaaacccc gtcacgctgc caaccaggat
     4261 ggttttcaaa attgctttaa gcaactccgc gccagtctga gccgagaaca tccgtttaat
     4321 gcccggtagc gggttgagtt tggaaaactt cggctgcaag gatttgccgc taaataccag
     4381 ccctcccagc atgaccggag aaatgagcgc caccagtacc acgccgctaa tcagtggcag
     4441 cagcgccagc atggcttctc tgatcagcag aataatctgc ccgaggatca gattcgggtc
     4501 tttgataata ctgtgatcaa aatgcagccc agcggagagc atgcccgaca atcgacgggc
     4561 cagcgacaca ccgccaaacc agataacact aacgcccacc agcaaaatca gcagtgaggt
     4621 cagttcacgg gaacgcggga tttgcccctc ttcccgcgct ttttctagtc ggtgaggtgt
     4681 gggggcttct gttttgtcgt cgctctcgtc agacacgtcg ccaatcctgg aaagagtatt
     4741 aagcgtgaat gatgccagag cgcaaagcgt tcaatggttt gagtaagggg caaaacaggc
     4801 gggatttagg gcttttgctg ccacacatca agcatagtgt gcgtttgtcg gatgcggcgt
     4861 catcgcctta tcagaccgcc tgatatgacg tggtcacgcc acatcaggca atacaaatca
     4921 aaatccaaga ctatccaaca aatcgtccac ctgatcctga ctggctacca caccggcttt
     4981 gctggtatcg acctgaggtc cattaagcaa actctggttt tcacgttttg gacgcgactc
     5041 ctgttccggg atgttttcca acagcaccat cagcaactgg cgttcgatct cctgaatgac
     5101 atccatcatc cgcttaatga cctgcccggt gagatcctga aaatcctgcg ccatcatgat
     5161 ttccagcagt tgcgcgttag taaagctggt atgcgcgggt acatctgcca gaaattgtcg
     5221 tgtatctgtt accagttcac gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc
     5281 ccaacgttgg gttaacgctt ttgctgattt ctccatttga tcctgatgcg gttgtgacgc
     5341 ctcaacactg ttcagcgccc gctccgcagc ctgggcggtc atctgcacaa catagtacaa
     5401 acgatcgcgc gcatcgggga tggcttccgc cgcttcggca atggcctgat ccagccccag
     5461 ttcccgcaaa ctgtcgcgca gcatacgcgt caggctgccg atgcgcgcaa tgatatcgcc
     5521 agctgaatgc tcgtcagcag gtttgattga tggttgcatc atagtcgcat cctcacatgc
     5581 ccagtttctc aaagattttg ttgagttttt cctccagcgt cgcggcggta aatggcttca
     5641 ccacatagcc actggccccc gcttgcgccg cagcaatgat gttctctttc ttcgcttctg
     5701 cagtcaccat taacactggc aatgccgaca tcgcgccatc cgcacgaatt gttttcagca
     5761 attccaggcc atccatattg ggcatgttcc agtcggagat aacaaatcca taaccgcctg
     5821 cctgcaactt attgagagcg tcgacgccat cttccgcttc ctcaacatta ttgaatccca
     5881 gctctttcag caggttacgc actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa
     5941 atttaagttc tttatccgcc atttcacact cctgatttaa atacgtatcg cctgtccggc
     6001 actaattttt gccagcattt gctggcttac ctggctaaga tcgaccactt cgcaaacacc
     6061 acccatattg atggcctcgc gcggcatgcc gaacaccacg caacttgctt cgttttgcgc
     6121 aagggtccat gcccccgcct gacgcatcgc caacattccc gccgcgccgt cgttgcccat
     6181 accggtcagg atcaccccaa ccgcattacg ccccgcctgt ttggcgacag aatggaacaa
     6241 cacatctacc gaaggccgat gacggttaac cgccgggcca tcgtgaattt tgatttggta
     6301 atttgcgcca ctacgcgcca gctccatatg ccgatcgccc gacgcaatat aggcatgccc
     6361 cggcaagaca cgttctccgt cttcggcttc tttaaccccg atctggcaaa gcttattaag
     6421 tctgtcggca aaagagcggg tgaaaccggg cggcatatgc tgggtaatta acagtgccgg
     6481 gctggaaagc ggcaacggtt gcagtacgtg acgaattgcc tcagttccac ccgttgaagc
     6541 accaatcgca atcagttttt cagaactcaa caacggcccc gccttcagcg ttgtcggtgc
     6601 cgacaatggc ttatgtgctg caaggctcgc ctttgctgcc gtacgcacct tttcagcaat
     6661 catttcgcta tacgccagca taccttcgcg aatacccagt tgcggtttgg tgacaaaatc
     6721 tatcgccccc agctccagcg cgcgcagcgt gacttctgac cctttgccgg tcagggaaga
     6781 aaccatcaca acgggcattg gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc
     6841 catccgcggc atttcaacat ccagcgtcag cacatcggga ttgaatttct taatcaagtc
     6901 acgcgcgacc agcggatcag gcgcggtcgc caccatttcc atgtcgctat ggctgttgat
     6961 gatttctgtc atgatctggc gcatcagtgc cgaatcatcg acagataaca ccctgatttt
     7021 gctcatcgtt aatccttact tagcgcatac accgtctgac cacgcagcgt gaagcggcgc
     7081 tcaaggtggc taaagttttc agagtgaccc gcaaacagca atccgtcggg tttaaggagc
     7141 ggaacaaagc ggcgcaaaat ctcctgctgg gtagtttgat cgaagtagat catgacgtta
     7201 cgacagaaga tcgcatcaaa cggccccggc acggtgtact gtttcgccag tagattcagc
     7261 ggggcaaaat caacatagtt cgccagctcc tgacgcacgc gtaccagccc ttcatgcggc
     7321 cccgtccctc gcatgaaata ccgttgcagt tgctgcggcg tcaggttttt caactcttca
     7381 tggcgataga taccgcttct ggctttttcc agcacttcgg tgtcgatatc actggcaaac
     7441 actttccagc gtccgggcgc ggtgcccaat gtgtcagcca gcgtcatcgc aatgctgtac
     7501 ggctcttcgc cggtcgaagc cgccgcgctc catacgcgat actcgccaga acggcgacgt
     7561 gcgtgatccg cgagcagagg gaaatgatgt gcctcacgga aaaatgccgt cagattcgtg
     7621 gtcagcgaat tgataaacgc ctgccactca ccgctgtgct gattagattc cagcaagttc
     7681 agataatgac cgaaatccgt cagtcccagt gaacgcaaac gacgaaccag tcggttgtaa
     7741 accatgtcgc gtttatggtc agccagaacg atcccggctc gttgatagat caattgactt
     7801 atccgccgaa aatgcgcgtc ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac
     7861 gtttgcccac agggcagaga tgaagtcata gcgccttctc aatcacttca ggataccact
     7921 ggcgcaattt gtaactgcac cgactcatgt cgtgctactt catgttcttc aagggtaaat
     7981 accgccacac gcgacgaaag atggtcggcc tgattcgcca gttgttccgt tgccaccgcc
     8041 gcttcttcta ccagcgaggc gttctgctga gtcacctgat ccatctggct gacagcctgt
     8101 gcaacctgct caatcccccg ctgttgttct tccgacgcag aggcaatttc tcccataatg
     8161 tcgttcacgc gggtcaccga actgacaata tcgatcatgg tcgcggcggc gttattcacc
     8221 agtttcgaac cctgctggac acgattgact gactcttcga tcagcccttt gatctctttt
     8281 gctgcctgcg cgctacggct ggcaagattg cgcacttcac ctgccactac cgcaaaacca
     8341 cgcccctgct ctccggcgcg agccgcttcc actgccgcat tcagggccag aatattggtc
     8401 tggaaagcaa ttccgtcgat aacgctgata atgtcgccaa ttttttgcga gctggtggcg
     8461 atctcctgca tggtgtgagt catggtactg acctgaacac cgcccgcctg cgccgttgtc
     8521 gcggcatttt ttgccagttc cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg
     8581 gcggttaatt gctccatact ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta
     8641 cgacttgaga gatcgttatt gcctgcgaca atctccgcga taccaatgtg catctcctgg
     8701 cttcccttac gcacatcact taccgtccca cgcaaagcct gctgcatggt cttcagactg
     8761 gcaaaaatgg cggtgatctc attacgacca tataccgcaa tcggacgcgc cagattaccc
     8821 gcagcaatgc tgtcaaaatg gctaccgata atggccagtg gttgaacaat catcttgcgc
     8881 gtccaccaca gcgcactgct gatgtagatc gccgcaacaa taatcatgct gataaacacc
     8941 agtgccgaaa tctgatagtt acgctggctt tgcgcactgg cggcttccag cacatggttg
     9001 atctccagct gccaggcctc aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc
     9061 gcagtgagga aatccgaaag ttgattactt tcaagccagg tcgcctgatg ttcgagatcg
     9121 ttgtgccagc gggcaaaact tttctccgtc tctttttgca atcccctgac gtgctcgttg
     9181 cccgcagtca tcgccataaa acttttaaac agcgtggtgg attgcgtcag actggcgcgc
     9241 gccgtcgtca tcaacgtttt aatgtcatcc gccggatagc taagtgccgt cagagtgccc
     9301 gctttgttca gcgcggtact ggcctgtaac attaccgccc gagtttgcgc taatgccgca
     9361 cgttgctgat tgctctgctc gacctgattc agtcgttgca aatcgtcgcg aaatgcccaa
     9421 aaagacatgc cgttactgcc aatctgcaag atcccgcaga gaatcaaaat taaaaacagc
     9481 gtggtcgaaa ttcgaatacg attaaacatc aacgctcccc atcaggcggc aatgaccgcg
     9541 ttagtaaata ctcgtcaaaa tgtttcccag tttggatctt gttcagcaat tcgcagtcgt
     9601 ggctgtgccg gtggttgctc actggcagga cgggatggtg tttgcggttt attggtgagt
     9661 gggctggctg ccagacggaa cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc
     9721 agcgcagcgg cggcggcagc tgattcctgc accagcgatg cgttctgttg cgtgacgcga
     9781 tccatttccg aaaccgccaa tgcgacttga tcgatgccac ggctctgttc atccgatgcc
     9841 gatgcaatct cgcccataat gtcagtcacg cgagtgacag cattgacgat attgttcatt
     9901 gtttccccgg cgctttcgac cagcaccgaa ccggtatcaa cgcgtgagac ggagtcttca
     9961 atgagggctt tgatctcttt tgccgcctgg gcgctgcgac tggcaagatt acgcacttca
    10021 cccgccacca cggcaaaacc acggccctgt tcacccgcac gcgcggcttc aaccgcggca
    10081 ttcagcgcga ggatattagt ctggaaggca ataccgtcga taacgctgat aatgtcggca
    10141 attttcttcg aactatcggc gatctcatgc atcgttttca ctacgccatc caccactttg
    10201 ccgccgtgct gggcggtgtc ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta
    10261 tcggcgtttt gcttcactgt cgcggtgagc tgctccatgc tggcggcagt ttcttccagc
    10321 gcggatgcct gctgttcagt acgggaggaa agatcggtgt tgcccgccgc aatttcacgg
    10381 gtaccggcat agatggcatc tgaaccttcg cggacatgag tgacggtgtc agtcaaagag
    10441 cgttgcatat gtgaaacgct ctgcgccagg tcgcccattt cactgcgccc gtcaatggtc
    10501 agggtattcg ccaggttacc accggcgatt tcgcgaatgt gagcaataat ttttgccagc
    10561 ggagtaagca acatacggcg aatgccgtac cacgccacca gcagaatcaa taccaccacc
    10621 agcgcgataa ccgccagttg ccactgggca aatcggtaat catctgcgtt gtcagtgacg
    10681 atatcgcgat acagtttttc actgctgagg gcgtactgag caaacgcttc gcccattgca
    10741 ttttgcattc cctgggttgg ctgagcgaaa taagctccag tattgccata atctagataa
    10801 tcaatcagtt cagttaacgc tgtgtaatag tttttatatt tttcatcaat attacgactg
    10861 gtagcgacca tttcaggtaa cggtgccatg cttttgaatt ttttataatg cgtcgctgcc
    10921 tgcgccaatg ttttcctggc gctatcgagc aattcaactt tggcgttact ttgttgattg
    10981 gaggaatcca tcatcatccg taccgctgaa cgactcaggt taatgcgcgt ttgcagcatt
    11041 aaatcccagg ttgacgtcag ctcgccctgc tgttcccgta attgattgga aaccacaaag
    11101 ctcttctggc tatggtgaag ggaagaaaaa aacagactgc cggaaataag ctgtaacagt
    11161 gcgaataccc ccagcaccat taccaacagc gtgactacgc ggatacggtt aatcataagg
    11221 caccttcctg aaaacaagtt gatctcgtta tcggcaagaa ggggggaaac tttattgctg
    11281 atgccacccg ccgcgaaatt gaaataaaaa acccgatgcg cagatcatcg ggttcatttc
    11341 aattgaggaa atcgggagaa ttacgccact tctgacgccg cgctatctaa cagcgccatc
    11401 tcttcgctgt tcagcagttt ttcgatgttc accagaatca acatccggtc gcccagtgcg
    11461 cccagtccag tgagatattc tgttgaaagc gtcacggcaa attccggtgc cggacgaatt
    11521 tgctccgccg tcaatgaaag cacgtctgag actccgtcaa ccacgatgcc gaccacccgc
    11581 tgtccgagat tcaggacgat aactaccgtg ttgtcgttat agtccacatc cacctggctg
    11641 aacttaattc gtaagtcaac aatcggcaca ataacgccgc gcagattcgt gacgcctttg
    11701 ataaacgctg gcgtgttcgc aatccgtgtt acctgatcgt agccacggat ctcctgcact
    11761 ttcaggatat caataccgta ctcttcatca ccaagggtaa ataccagaaa ttcctggcct
    11821 gacggctcgc tggccagctt tgttacattc gtcataccgg tcatattgtt acctttttac
    11881 tcattcaggc ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga
    11941 catcaacaat cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa atgccgggga
    12001 ctttgcgata gttactttca aggtttttaa ccacaacctg gtgttgacca attaattgat
    12061 ccaccagcaa ggcgtagcgg cgaccgccac tttgtaagat caccacaatt ccctgggtgg
    12121 cttcggtttt cgcgcccgcg acgttgaaca ctttccacag ttcgacgatg ggcagatatt
    12181 caccccgcac ttccagcacc cgctcgccgc cggccagtgg atggagatcg gcttcacggg
    12241 gttgcagtga ttccataaca gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta
    12301 cggacatgcc gtcgaggatg gccagcgtca gcggcagtaa aatgcggatc gtagtgccag
    12361 taccctgctt cgactggatt tcgacatgac cgcccatctc ctggatatta cgtttgacga
    12421 cgtccatgcc gacgccgcgc ccggagacgt cggtgacctg ctctgccgtg gagaagccag
    12481 gtgcaaatat cagcatcgcg acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt
    12541 gcgaggccgc ttttgccaga attcgctcac ggtttagccc cgccccatcg tcggtcactt
    12601 caatgcaaat gttgccgccc tgatgttcgg cagacagaat taaatttccg acgctgtttt
    12661 tacctgcggc gagccgtttt tctggcagtt caataccgtg atcgaggcta ttgcgtacca
    12721 ggtgggtcag cgggtcgata atgcgttcta tcaggctttt gtcgagttca gtagaactgc
    12781 ccaccagcgt cagttctacc tgcttgccga gttttcccgc cagatcacgc accagccggg
    12841 gatagcgact aaaaacatat tccatcggca tcatgcgaat cgacatcact gattcctgca
    12901 aatcacgggc gttacgttgt aactgcccca tgctggttat caaatcacca tgattaaccg
    12961 ggtccagttc gctggaacgc tgggcaagca tggactgggt gataaccagc tcgccgacga
    13021 ggttaattaa ttgatcaacc ttttctaccg ctacacggat gctggtggat tcattgctgc
    13081 gcgtcgtttt ttcccgctcc acgcggccgg ttggcgcttg ttcggctgcc agtttaagca
    13141 ctggtggggt ggatattttt ggcgagactt ctactgtttc aaaggtaatc tgatcggctt
    13201 caatcacaaa acagagtacc gctgtgatgt catcttcggc gatgtcgccc ggtaatattg
    13261 ccgagagcga atccgcccct ttcaccacgt cagttaactt tgtcagatgt cccagttctt
    13321 cttccagcag gtcgacttcc ccggccttca ggcgcgaaag gataattcgt cgcggcgact
    13381 gactgcgact ctgctcatct tgcggttcac ttttggcaac cacacttaat cgggtcactg
    13441 cggatggcgt ttcgcctttc gcttctaatg ccagttgacg caaggcctgg cagatataat
    13501 cgaagctggc ggcatccggc tcttgcgact gtttataagc gtcgagctgt tcttgcatga
    13561 tgtccttcgt ttccaaaaac agattgataa tgtcggtgtt gagttgcatc tcacctcgtc
    13621 tggcttcatc gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa
    13681 aagttcctgc ccctcctttg atcgagtggg cagcccgaaa gatggcattc aattgttcgg
    13741 catctggcgc ttccggctgc aaaaccagca aatgctgctc catgtcagcc aacagttcgt
    13801 ccgcttcatc aaaaaatgtc tgataaaaat cgcttatatc catgctcacg ctgtcacctc
    13861 ggttcggctg atggcattgt gggaacactg acctgtggtg caacctcagg tttttccagg
    13921 gcgcttactg gctcattctg gctttcggcg ttttcatgca aaatggcctg ttcggcttgt
    13981 ttgttcagta ccagcaggct gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt
    14041 aagcgcatcg ttgccgccat gccgacgaca cgtaacactt tgccgctatc caaccctccg
    14101 accatcagtt cgcggcggga tgcattggcc cgatcggcag aaagctccca gttgctatat
    14161 cctttctcac cgctggcgta ggggaaatca tcggtatgac ctgaaaggct aatacggttg
    14221 ggaataccgt tcagtacagg cgcaatggcg cgcagaatgt cgcgcatata gggttcgaca
    14281 tcggcactgc cggttctaaa catcgggcga ttctggctat cgatgatctg aatacgtaga
    14341 ccttcctgga ccagatcgat tttgagatgg ggacgtaacg cccgcagttt cggatcggac
    14401 tctatcaact ggtcgagatc accccgcaat ttccgcaatc gactttgctc catgcgtttt
    14461 ttcagctctt cgatgttcgg ctgcttattc acttccccct ggctttgggt gtaatcatca
    14521 ccaccgccgg gaattgggct ttcactatta gaaatgcgat cgccgcccgt aaccgcagtc
    14581 gccagtggag tccggaagta ctccgcaatc tgaatcagct cttttgggct ggagatggag
    14641 atcagccaca tcaccagaaa aaaggccatc atcgcagtca taaagtcggc ataagcaatc
    14701 ttccacgatc catgtgctgc cccgtggctt ttggctttgc gtcgtttgac gacaataatc
    14761 ggatgcgctt gattcttcat gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg
    14821 cacgcacatg ctcttccagt tcaataaacg acggacgttc gctggaatag agcgttttgc
    14881 gaccaaactc aacggcgata ggcggtgcgt aaccgttcag attagaaagc agagtgactt
    14941 tgacgcactg catcattttg ctggt
//
LOCUS       DACAPB010000113        14854 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.113, whole genome shotgun sequence.
ACCESSION   DACAPB010000113 DACAPB010000000
VERSION     DACAPB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.113"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1015
                     /gene="secY"
                     /locus_tag="IT904_003042"
     CDS             <1..1015
                     /gene="secY"
                     /locus_tag="IT904_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP3192787.1"
                     /translation="KEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVI
                     NPGFAFYFTAVVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIE
                     QARQGDLHFLVLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLP
                     LKVNMAGVIPAIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASA
                     IIFFCFFYTALVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYIT
                     FICLIPEFMRDAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKG
                     YGR"
     gene            1047..1163
                     /gene="rpmJ"
                     /locus_tag="IT904_003043"
     CDS             1047..1163
                     /gene="rpmJ"
                     /locus_tag="IT904_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP3192788.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            1310..1666
                     /gene="rpsM"
                     /locus_tag="IT904_003044"
     CDS             1310..1666
                     /gene="rpsM"
                     /locus_tag="IT904_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP3192789.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            1683..2072
                     /gene="rpsK"
                     /locus_tag="IT904_003045"
     CDS             1683..2072
                     /gene="rpsK"
                     /locus_tag="IT904_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP3192790.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            2106..2726
                     /gene="rpsD"
                     /locus_tag="IT904_003046"
     CDS             2106..2726
                     /gene="rpsD"
                     /locus_tag="IT904_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP3192791.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            2752..3741
                     /gene="rpoA"
                     /locus_tag="IT904_003047"
     CDS             2752..3741
                     /gene="rpoA"
                     /locus_tag="IT904_003047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP3192792.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            3782..4165
                     /gene="rplQ"
                     /locus_tag="IT904_003048"
     CDS             3782..4165
                     /gene="rplQ"
                     /locus_tag="IT904_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP3192793.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            4272..4640
                     /locus_tag="IT904_003049"
     CDS             4272..4640
                     /locus_tag="IT904_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAP3192794.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            4651..5076
                     /gene="zntR"
                     /locus_tag="IT904_003050"
     CDS             4651..5076
                     /gene="zntR"
                     /locus_tag="IT904_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAP3192795.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            5132..5350
                     /locus_tag="IT904_003051"
     CDS             5132..5350
                     /locus_tag="IT904_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAP3192796.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(5347..5757)
                     /gene="mscL"
                     /locus_tag="IT904_003052"
     CDS             complement(5347..5757)
                     /gene="mscL"
                     /locus_tag="IT904_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP3192797.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(5887..7263)
                     /gene="trkA"
                     /locus_tag="IT904_003053"
     CDS             complement(5887..7263)
                     /gene="trkA"
                     /locus_tag="IT904_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAP3192798.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(7285..8574)
                     /gene="rsmB"
                     /locus_tag="IT904_003054"
     CDS             complement(7285..8574)
                     /gene="rsmB"
                     /locus_tag="IT904_003054"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP3192799.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKTGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(8620..9567)
                     /gene="fmt"
                     /locus_tag="IT904_003055"
     CDS             complement(8620..9567)
                     /gene="fmt"
                     /locus_tag="IT904_003055"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP3192800.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVAELQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(9582..10091)
                     /gene="def"
                     /locus_tag="IT904_003056"
     CDS             complement(9582..10091)
                     /gene="def"
                     /locus_tag="IT904_003056"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP3192801.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            10221..11345
                     /gene="dprA"
                     /locus_tag="IT904_003057"
     CDS             10221..11345
                     /gene="dprA"
                     /locus_tag="IT904_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP3192802.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            11317..11790
                     /locus_tag="IT904_003058"
     CDS             11317..11790
                     /locus_tag="IT904_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAP3192803.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            11819..12361
                     /locus_tag="IT904_003059"
     CDS             11819..12361
                     /locus_tag="IT904_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192804.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            12366..12938
                     /gene="tsaC"
                     /locus_tag="IT904_003060"
     CDS             12366..12938
                     /gene="tsaC"
                     /locus_tag="IT904_003060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAP3192805.1"
                     /translation="MNNNLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            12943..13761
                     /gene="aroE"
                     /locus_tag="IT904_003061"
     CDS             12943..13761
                     /gene="aroE"
                     /locus_tag="IT904_003061"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP3192806.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            13758..14015
                     /locus_tag="IT904_003062"
     CDS             13758..14015
                     /locus_tag="IT904_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAP3192807.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(13991..14545)
                     /locus_tag="IT904_003063"
     CDS             complement(13991..14545)
                     /locus_tag="IT904_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP3192808.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         3746 a         3543 c         3781 g         3784 t
ORIGIN      
        1 gaaagaaggg gagtctggtc gtcgtaagat cagccagtac acccgctacg gtactctggt
       61 gctggcaata ttccagtcga tcggtattgc taccggtctg ccgaatatgc ctggtatgca
      121 aggcctggtg attaacccgg gctttgcatt ctacttcacc gctgttgtaa gtctggtcac
      181 aggaaccatg ttcctgatgt ggttgggcga acagattact gaacgaggta tcggcaacgg
      241 tatttcaatc attatcttcg ccggtattgt cgcgggactc ccgccagcca ttgcccatac
      301 tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg ttgctgttgg ttgcagtatt
      361 agtatttgca gtgacgttct ttgttgtatt tgttgagcgt ggtcaacgcc gcattgtggt
      421 aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct gcacagagca cacatttacc
      481 gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc gcttccagta ttattctgtt
      541 cccggcgacc atcgcgtcat ggttcggggg cggtactggt tggaactggc tgacaacaat
      601 ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta ctctatgcgt ctgcaatcat
      661 cttcttctgt ttcttctaca cggcgttggt tttcaacccg cgtgaaacag cagataacct
      721 gaagaagtcc ggtgcatttg taccaggaat tcgtccggga gagcaaacgg cgaagtatat
      781 cgataaagta atgacccgcc tgaccctggt tggtgcgctg tatattacct ttatctgcct
      841 gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc tacttcggtg ggacctcact
      901 gcttatcgtt gttgtcgtga ttatggactt tatggctcaa gtgcaaactc tgatgatgtc
      961 cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa ggctacggcc gataattggt
     1021 cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc ttccgtcaag aaattatgcc
     1081 gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt gatttgcagt gccgagccga
     1141 agcataaaca gcgccaaggc tgattttttc gcatattttt cttgcaaagt tgggttgagc
     1201 tggctagatt agccagccaa tcttttgtat gtctgtgcgt ttccatttga gtatcctgaa
     1261 aacgggcttt tcagcatgga acgtacatat taaatagtag gagtgcatag tggcccgtat
     1321 agcaggcatt aacattcctg atcataagca tgccgtaatc gcattaactt cgatttatgg
     1381 cgtcggcaag acccgttcta aagccatcct ggctgcagcg ggtatcgctg aagatgttaa
     1441 gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt gacgaagttg ccaaatttgt
     1501 cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc aagcgcctga tggatcttgg
     1561 ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt cgcggtcagc gtaccaagac
     1621 caacgcacgt acccgtaagg gtccgcgcaa accgatcaag aaataatcgg ggtgattgaa
     1681 taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag aaaacaagtc tctgacggcg
     1741 tggctcatat ccatgcttct ttcaacaaca ccatcgtgac tatcactgat cgtcagggta
     1801 acgcgttggg ttgggcaaca gccggtggtt ccggtttccg tggttctcgc aaatccactc
     1861 cgtttgcagc tcaggttgca gcagagcgtt gcgctgacgc cgtgaaagaa tacggcatca
     1921 agaatctgga agttatggtt aaaggtccgg gtccaggccg cgaatctact attcgtgctc
     1981 tgaacgccgc aggtttccgc atcactaaca ttactgatgt gactccgatc cctcataacg
     2041 gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcctcgt tttccaggtt tgttggagaa
     2101 agaaaatggc aagatatttg ggtcctaagc tcaagctgag ccgtcgtgag ggcaccgact
     2161 tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg taaaattgaa caagctcctg
     2221 gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg tgtgcagttg cgtgaaaagc
     2281 aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt ccgtaactac tacaaagaag
     2341 cagcacgtct gaaaggcaac accggtgaaa acctgttggc tctgctggaa ggtcgtctgg
     2401 acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc agaagcacgt cagctggtta
     2461 gccataaagc aattatggta aacggtcgtg ttgttaacat cgcttcttat caggttagtc
     2521 cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca gtctcgcgtg aaagccgctc
     2581 tggagctggc tgagcagcgt gaaaagccaa cctggctgga agttgatgct ggcaagatgg
     2641 aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc tgcggacatt aacgaacacc
     2701 tgatcgtcga gctttactcc aagtaaagct tagtaccaaa gagaggacac aatgcagggt
     2761 tctgtgacag agtttctaaa accgcgcctg gttgatatcg agcaagtgag ttcgacgcac
     2821 gccaaggtga cccttgagcc tttagagcgt ggctttggcc atactctggg taacgcactg
     2881 cgccgtattc tgctctcatc gatgccgggt tgcgcggtga ccgaggttga gattgatggt
     2941 gtactacatg agtacagcac caaagaaggc gttcaggaag atatcctgga aatcctgctc
     3001 aacctgaaag ggctggcggt gagagttcag ggcaaagatg aagttattct taccttgaat
     3061 aaatctggca ttggccctgt gactgcagcc gatatcaccc acgacggtga tgtcgaaatc
     3121 gtcaagccgc agcacgtgat ctgccacctg accgatgaga acgcgtctat tagcatgcgt
     3181 atcaaagttc agcgcggtcg tggttatgtg ccggcttcta cccgaattca ttcggaagaa
     3241 gatgagcgcc caatcggccg tctgctggtc gacgcatgct acagccctgt ggagcgtatt
     3301 gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg acctggacaa gctggtcatc
     3361 gaaatggaaa ccaacggcac aatcgatcct gaagaggcga ttcgtcgtgc ggcaaccatt
     3421 ctggctgaac aactggaagc tttcgttgac ttacgtgatg tacgtcagcc tgaagtgaaa
     3481 gaagagaaac cagagttcga tccgatcctg ctgcgccctg ttgacgatct ggaattgact
     3541 gtccgctctg ctaactgcct taaagcagaa gctatccact atatcggtga tctggtacag
     3601 cgtaccgagg ttgagctcct taaaacgcct aaccttggta aaaaatctct tactgagatt
     3661 aaagacgtgc tggcttcccg tggactgtct ctgggcatgc gcctggaaaa ctggccaccg
     3721 gcaagcatcg ctgacgagta accggatcac aggttaaggt tttactgaga aggataaggt
     3781 catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac agcagccatc gccaggctat
     3841 gttccgcaat atggcaggtt cactggttcg tcatgaaatc atcaagacga ctctgcctaa
     3901 agcgaaagag ctgcgccgcg tagttgagcc gctgattact cttgccaaga ctgatagcgt
     3961 tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac gagatcgtgg caaaactgtt
     4021 taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt tacactcgta ttctgaagtg
     4081 tggcttccgt gcaggcgaca acgcgccgat ggcttacatc gagctggttg atcgttcaga
     4141 gaaagcagaa gctgctgcag agtaatctga agcaacgtaa aaaaacccgc cccggcgggt
     4201 ttttttatac ccgtagtatc cccacttatc tacaatagct gtactctttt tgttcatccc
     4261 ctggagtatt tatgtggtta cttgaccagt gggcagagcg ccatatagca gaagcgcaag
     4321 cgaaaggtga gtttgataac ctggcaggta gcggcgaacc attgatactg gatgatgatt
     4381 ctcacgtgcc accggaatta cgtgcggggt atcgcttgct gaagaatgcc ggttgcttac
     4441 cgccagaact tgagcaacgg agagaagcaa ttcagcttct ggatattctc aaaggtatcc
     4501 gtcacgatga tccgcaatat caagaggtta gccgtcgatt gtcattactg gaattgaagc
     4561 tgcgacaagc tggattgagt accgattttt tacgcggcga ttatgctgac aagttgttgg
     4621 acaaaatcaa cgataactag tggagtatgt atgtatcgca ttggtgagct ggcaaaaatg
     4681 gcggaagtaa cacccgacac gattcgttat tacgaaaaac agcagatgat ggagcatgaa
     4741 gtgcgtactg aaggtgggtt tcgcctatat accgaaagcg atctccagcg attgaaattt
     4801 atccgccatg ccagacaact aggtttcagt ctggagtcga tccgcgagtt gctgtcgatc
     4861 cgcatcgatc ctgaacacca tacctgtcag gagtcaaaag gcattgtgca ggaaagattg
     4921 caggaagtcg aagcacggat agccgagttg cagagtatgc agcgttcctt gcaacgcctt
     4981 aacgatgcct gttgtgggac tgctcatagc agtgtttatt gttcgattct tgaagctctt
     5041 gaacaagggg cgagtggcgt taagagtggt tgttgatttt ttgcactggc aggattacac
     5101 tcgcgccgtt aaataaccaa ctggagtttt tatgagtcga tatcagcata ctaaagggca
     5161 gataaaggat aatgcgatag aagcattact acatgatccc ttattccgac agcgcgtaga
     5221 gaaaaataag aaggggaaag gcagttacat gcgaaaagga aaacatggca atcgggggaa
     5281 ctgggaggcc agtggcaaga aagtaaatca cttttttacc actggtcttc tgctttcagg
     5341 cgcttgttaa gagcggttat tctgctcttt cagcaaatca cgaatttctg tcagtaatac
     5401 ttcttcttta gttggtgcag gtgcggctgc tggttcttct tttttccgat tcagtttgtt
     5461 gattagctta atcgccataa agatggcaaa ggccacaatc agaaaatcaa agacgttttg
     5521 aatgaagaca ccgtaatgca tcacaacagc agggatatcc ccctgcgcat cgcgtagcgt
     5581 gacagcaaac tgtttaaaat cgatcccgcc aattaataag cccagaggag gcatgatgat
     5641 atcggcaacc agtgaagaga caatcttccc gaatgccgca ccgataatga cacccaccgc
     5701 caaatccacc acgttcccgc gcatcgcaaa ttcgcgaaat tctttaataa tgctcatgtt
     5761 attctcccta tgaagccgac aaccataagt ctaacaaatg ttaagccatt ttcctgccag
     5821 gaatgaattt aattaatcct tgataatcaa agggaaataa taaggcgtca ttagacgcct
     5881 tattaattac aagaagaaag ggcttggctg gaagagtctt tcgacgtcgg taataaactt
     5941 tttatctgtg aggaacataa ttacgtgatc gccttgctca atgcgcagat tgtcattggc
     6001 aatcatcacg tcgtttccac gtaccaccgc tccaataatc gttcctggcg gtagcttgat
     6061 ttcgtcaatg actctgccga caacgcgtga ggtgctttca tcaccgtgag caacggcttc
     6121 aatagcttct gctacgccgc ggcgcaatga ggaaacacca acaatatctg cttttcgcac
     6181 atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa atcgcaatat cgataacgct
     6241 cccctgaacc agatccacat aagcgcgacg ctggatcaat accatcacct ttttcgcacc
     6301 catacgtttg gcaagcatgg cggacatgat attggcctcg tcatcgttgg tgacggcaat
     6361 aaacagatca acttgatcga tatgttcttc ggccagtagt tcttgatccg acgcatcacc
     6421 aaaaaagacg atcgtattct gtaacttttc cgccagttcg gcagcgcgct gctgattacg
     6481 ttcgatgagt ttaacgctgt aatctttttc cagacgacgc gccagccctg caccgatatt
     6541 accgccacca accagcatga tccgcttata cggtttttcc agtcgctgta attcactcat
     6601 caccgcgcgg atatgctgtg aagcggcaat aaagaacact tcatcaccag cttcaacaat
     6661 ggtcgaacct tgcggacgaa tggggcgatc gtggcggaaa attgctgcca cacgagtatc
     6721 gatatgtggc atatgttcgc gcatggtcga aagtgcatta ccaatcagcg ggccgccata
     6781 ataggcttta accacagcca ggctgacttt accctcagcg aagttcacca cctgcaatgc
     6841 gccgggatac tcaatcagtc ggtaaatatt atcgataacc aactgctctg gtgcgatcag
     6901 atgatcaatc ggcacagcat ctgaatgaaa gagcttatcg gcatcgcgca cgtagtctgg
     6961 tgagcggata cgagcgatgc gattaggggt gttgaaaagt gagtaggcta cctggcaggc
     7021 aaccatattg gtttcatctg aactggttac agcaaccagc atatcggcgt cgtcggcacc
     7081 tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc acgacccgca ggtcaaattt
     7141 atcctgtaag gtccgcagac gctcaccgtt ggtatcgaca acagtaatat cgttgttctc
     7201 gccaaccagg ttttccgcca gtgtgccgcc aacctggccg gcacccagaa tgataatttt
     7261 catcagtcgc gacccgttat ctcatcactt tttgattagc ttagcgtaaa agaagccgtc
     7321 gccctcttcg gcaccaggta gattttgttt acccggttgc tctggtgttc ctgtttcgca
     7381 aagttcggca tcagcggtac gttgcaaaaa ggctttaatc tgcaggctat tctcttccgg
     7441 taacaccgaa caggtggcat agaccagagt tccaccggtt tttaaatgcg gccaaatggc
     7501 gtcgagaatt tcagactgca attgcgcgag ttccgggata tcgcgatcgc gacgtaacca
     7561 tttaatatct ggatggcgac gaatcacacc ggttgctgaa caaggcgcat ctaataaaat
     7621 gcgatcaaac tgttgctcgc cacaccattg agaagggtaa cggccatcac cttgtttcac
     7681 ggtcgccttc ataccaaggc gttttaaatt gtcgtaaacc cgagagaggc gctgttcgtc
     7741 gatatcaacc gcaacaacct gcgcttctgg tgccacctca aggatatgcg ttgttttacc
     7801 gccgggggcg gcacaaagat ccaaaatgtg ttcaccgttt tgtggcgcaa gccaggtcat
     7861 gcaaccttgt gctgatgcat cctgaacggt aacccatccg tcttcaaaac caggtagcgc
     7921 atgaacaggt gcaggtgttt ccagacgtac agcatcaggg taatccgcat gcgggaaacc
     7981 tttcattcct gcttcatcca gcaatgcaag ccagctgtcg cgggaatgat gcgtacgatt
     8041 aatacgcagc cacattggcg gacgctggtt attggcttcg acgatggatt gccactgctc
     8101 tggatacgct ttttgcagac gcttcagcaa ccaggaagga tgcagataac gtgcatcact
     8161 ggcattaaac tcggctaata actcttcttg ctgacgctgg aactggcgta atacaccgtt
     8221 tatcaaccct ttaagttgcg gacgcttaat tgcgatagcg ccttcaaccg tttcagccag
     8281 cgcagcatga ggtggaatgc gggtataaag cagttgatac aaaccaacca taatcaggta
     8341 atgcacggtc cgctgtttgc cggtcatcgg acgggccatt aacttattaa tcagccagtc
     8401 taactgcgaa agcgtacgca gtacgccaaa gcacaactct tgaagaagtg ctttgtcttt
     8461 atcggaaact ttttgctgga gcggtggcag aatgttgctt aatgattgcc cttgctcgac
     8521 gacttgttca acggcctggg ccgccatgct acgtaaatta cgttgttttt tcataagtat
     8581 aaaaacgccc ggcaagaccg ggcttagaag agtggactat cagaccagac ggttgcccgg
     8641 aacaaaccat tcccgacgag agttcaggag gtcttgcgcg ctcatcgctt tcttacccgc
     8701 aggttgtaac gagagcaggt tcaggatgcc atcaccagtc gcaacctgaa tgccttgttt
     8761 gttggcttca aggatcgttc ctggtgcagc gttggttgcc gtatcaatga ccgatgcttt
     8821 ccagacttta accggctgtc cttcaatttc cagccagctc attggccatg gattgaaagc
     8881 gcgaatgcag cgttcaagct gtgctgccga aagtgaccag tcaatacgcg cttcttcttt
     8941 actcaacttc tcggcgtaag tgacaagagt ttcgtcctga acttctggtt tcgccgtgcc
     9001 gtctgccagt tgtttcaacg tggtgataag cccttgtggg ccaagctctg ccagcttgtc
     9061 gtacagcgta ccactggtat cttctgcagt aatcgggcag gagagcttat agagcatatc
     9121 accggtgtct aaaccgacat ccatttgcat aatggtcaca ccagtttctg catcacccgc
     9181 ccatagtgag cgttggattg gtgcagcacc gcgccagcgt ggcagcagtg aaccatgaac
     9241 gttgatacag ccaagacgcg gcatctccag cactgctttc ggcagaatta aaccataggc
     9301 gacgacgacc ataacatcag cctgcagttc ggcgaccagt tgctggtttt cttgtggacg
     9361 cagggaaaca ggttgaaaaa cgggcagacc tttttcctca gccagaactt taaccgggct
     9421 gggcatcagt tttttaccgc gtcctgccgg tcggtctggc tgggtgaaca cgccaacgac
     9481 gttatgacca gaagacaaca gcgcgtcgag atgacgcgct gcaaagtcag gtgtacccgc
     9541 aaaaataata cgtagtgatt ctgacacgtt agttcttatc cttaagcccg ggctttcaga
     9601 cgatccagtt tttcaacttt ctgacgaata cgttgttgtt tcagcggtga cagataatcc
     9661 ataaacagtt tgccgaccag gtgatccatc tcatgctgaa tacagatggc taacagaccg
     9721 tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg cgcgaatttt aactttctct
     9781 gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc aaccttcttc aatgcctgtt
     9841 tcgccgcttt tttctaaaag ctctggattg attaacacta gccgttcgtc acggttttcc
     9901 gaaacatcaa taacaatgat acgttgatgg atatcaacct gggttgccgc caggccaata
     9961 ccttcttctg cgtacatcgt ctcgaacata tcatcgacga tacgctgaat ttctgcattc
    10021 acttcttcta ccggtttagc aactttgcga agccgctcgt ccggaatatg taacacttgc
    10081 aaaactgaca taaatctcca gagatgtgtt caggagttag aaagattatt tcttctattc
    10141 tagacaaatc cccctctgat tgacagcatc actgaccaat cgcaaagatt gctaaggctg
    10201 cttatggcag ggagataagg atggtcgata cagaaatttg gctgcgttta atgagtatca
    10261 gcagcttgta cggcgatgat atggtccgta tcgctcactg ggtggcaaaa cagtcgcata
    10321 ttgatgcggt tgtattgcag caaacagggc ttacattgcg gcaggcacaa cgctttcttt
    10381 catttccacg aaagagtatc gaaagctcac tttgttggtt ggagcaaccc aaccatcatt
    10441 taattcctgc ggacagcgaa ttttatcctc ctcaacttct ggcgacgaca gattaccccg
    10501 gcgcactgtt tgttgaagga gaactgcacg cgctgcattc atttcagctt gccgtagtgg
    10561 ggagtcgggc gcattcatgg tatggcgagc gatggggacg attattttgc gaaactctgg
    10621 cgacgcgtgg agtgacaatt acgagtggac tggcgcgtgg aatcgatggt gtagcgcata
    10681 aagcagcctt acaggtaaat ggcgtcagca ttgctgtatt ggggaatgga cttaatacca
    10741 ttcatccccg ccgtcatgcc cgactggctg ccagtctgct tgaacagggg ggcgctctcg
    10801 tctcggaatt tcccctcgat gttccacccc ttgcttacaa tttcccacga agaaatcgca
    10861 ttatcagtgg tctaagtaaa ggtgtactgg tggtggaagc ggctttgcgt agtggttcgc
    10921 tggtgacagc acgttgtgcg cttgagcagg ggcgagaagt ttttgccttg ccaggtccaa
    10981 tagggaatcc gggaagcgaa gggcctcact ggttaataaa acaaggtgcg attcttgtga
    11041 cggaaccgga agaaattctg gaaaacttgc aatttggatt gcactggttg ccagacgccc
    11101 ctgaaaattc attttattca ccagatcagc aagacgtggc attgccattt cctgagctcc
    11161 tggctaacgt aggagatgag gtaacacctg ttgacgtcgt cgctgaacgt gccggccaac
    11221 ctgtgccaga ggtagttact caactactcg aactggagtt agcaggatgg atcgcagctg
    11281 tacccggcgg ctatgtccga ttgaggaggg catgccatgt tcgacgtact aatgtatttg
    11341 tttgaaacct atattcacac agaagctgag ttgcgtgtgg atcaagacaa acttgaacag
    11401 gatcttaccg acgcaggttt tgagcgagaa gatatctaca atgccctgct atggctggaa
    11461 aaacttgctg attatcagga agggttggca gaaccgatgc aactggcctc ggatcctctc
    11521 tccatgcgta tttatacacc ggaagagtgt gaacgactgg atgccagctg ccgtgggttt
    11581 ctgcttttcc ttgagcagat tcaggtgctc aaccttgaaa ctcgtgaaat ggtgatagag
    11641 cgagtgctcg cgctggataa cgcagagttc gaactggatg atctgaaatg ggtgatcctg
    11701 atggtgttgt tcaatattcc gggctgcgaa aatgcgtacc agcaaatgga agaattactc
    11761 tttgaagtga atgaaggtat gctgcattaa tcatttcttt aattcagcat aagttgttat
    11821 ggcgaaatca gcactgttca cggtgcgtaa taatgagtcc tgcccaaagt gcggggctga
    11881 actggttatt cgatccggga aacacggtcc gtttcttgga tgctcacagt atccggcgtg
    11941 tgactacgtc cgtcctctga aatcttcagc ggatggacat atcgtcaaag ttctggaggg
    12001 gcaggtttgc cctgcatgtg gcgcaaatct ggtattacgc cagggacgct ttggtatgtt
    12061 tattggttgc attaactacc ctgaatgcga acataccgaa cttatcgata aaccggacga
    12121 aacagcaatt acatgccccc aatgtcggac gggccatctg gtccagcgcc gctcccgtta
    12181 tggcaaaaca tttcactctt gtgatcgcta cccggagtgt caatttgcca ttaacttcaa
    12241 acccatagct ggagaatgcc ctgagtgtca ttatccgcta ctcatcgaaa agaaaaccgc
    12301 gcagggtgta aaacactttt gtgccagtaa acaatgtgga aagccggttt cggcggaata
    12361 ataacgtgaa taataacctg caaagagacg ctatcgcagc tgcgatagat gttctcaatg
    12421 aagaacgtgt catcgcctat ccaacggaag ccgttttcgg tgttgggtgc gatcctgata
    12481 gcgaaacagc agtgatgcga ctgttggagt taaaacagcg tccggttgat aaggggctga
    12541 ttttaatcgc agcaaattac gagcagctta aaccctatat tgatgacacc atgttgactg
    12601 acgtgcagcg tgaaaccatt ttttcccgct ggccaggtcc tgtcaccttt gtctttcccg
    12661 cgcctgcgac aacaccgcgc tggttgacgg gccgctttga ttcgcttgct gtacgagtca
    12721 ccgaccatcc gttggtggtt gctttgtgcc aggcttatgg taaaccgctg gtttctacca
    12781 gtgccaactt gagtggattg ccaccttgtc gaacagtaga cgaagttcgc gcacaatttg
    12841 gcgcggcgtt cccggttgtg cctggtgaaa cgggggggcg tttaaatcct tcagaaatcc
    12901 gcgatgccct gacgggtgaa ctgtttcgac aggggtaaca taatggaaac ctatgctgtt
    12961 tttggtaatc cgatagccca cagcaaatcg ccattcattc atcagcaatt tgctcagcaa
    13021 ctgaatattg aacatcccta tgggcgcgtg ttggcaccca tcaatgattt catcaacaca
    13081 ctgaacgctt tctttagtgc tggtggtaaa ggtgcgaatg tgacggtgcc ttttaaagaa
    13141 gaggcttttg ccagagcgga tgagcttact gaacgggcag cgttggctgg tgctgttaat
    13201 accctcatgc ggttagaaga tggacgcctg ctgggtgaca ataccgatgg tgtaggcttg
    13261 ttaagcgatc tggaacgtct gtcttttatc cgccctggtt tacgtattct gcttatcggc
    13321 gctggtggag catctcgcgg cgtactactg ccactccttt ccctggactg tgcggtgaca
    13381 ataactaatc ggacggtatc ccgcgcggaa gagttggcta aattgtttgc gcacactggc
    13441 agtattcagg cgttgagtat ggacgaactg gaaggtcatg agtttgatct cattattaat
    13501 gcaacatcca gtggcatcag tggtgatatt ccggcgatcc cgtcatcgct cattcatcca
    13561 ggcatttatt gctatgacat gttctatcag aaaggaaaaa ctccttttct ggcatggtgt
    13621 gagcagcgag gctcaaagcg taatgctgat ggtttaggaa tgctggtggc acaggcggct
    13681 catgcctttc ttctctggca cggtgttctg cctgacgtag aaccagttat aaagcaattg
    13741 caggaggaat tgtccgcgtg aatcaggcca tccagtttcc ggacagggaa gagtgggacg
    13801 agaataaaaa atgtgtatgt tttcccgctc tcgtgaatgg tatgcaactg acatgcgcga
    13861 tctctggcga gagtctggcg tatcgcttta ctggagatac gccagaacag tggttagcga
    13921 gttttcgtca gcatcgctgg gacctggaag aagaagcgga aaacttaatt caggaacaaa
    13981 gtgaagatga tcaaggctgg gtctggttac cctgatccag atattcgtcc ttccatttca
    14041 cgtaattatt cgcggaatag cgtaacccag ccttctcttc atcacttaac gggcggatct
    14101 gtttgacggg gctaccgaga tacagatatc cgctctccag ccgtttattt tgtgggacca
    14161 gactacccgc accaatcatc acatcatctt ctactattgc gccatcaagt aaaattgagc
    14221 ccatcccaac caaaactcga ttgccaatgg tgcagccgtg gagcatcacc ttgtgaccaa
    14281 cagtgacatc ttcgccaatg gttaatgggt tgccatctgg gttgtacgag gatttatgag
    14341 tgacatgcaa catactgcca tcctggatat tggtgcgtgc tccgatctgt acataatgta
    14401 catctccacg aatcacaacg agcggccaga tccccacatc atcagccaga cgaacgtcac
    14461 caatcacgac actgctatcg tcgatcatta cgcgctgacc gatttgtgga aaaagatcgc
    14521 ggtatgggcg taaaacatca gacatactta cctcagcaat aaatgattta ctaatgactt
    14581 tgggggcatt attggccttg tgcaagtctt ttagtatgca aaaaagcacc gttttgtgtg
    14641 cgattgcagc aaaaagggtg aaaaaacaac aaacagaaaa aaagatcaaa aaaatacttg
    14701 tgcaaaaaat tgggatccct ataatgcgcc tccgttgaga cgacaacgtg aaacacttca
    14761 caggatggtc ggaacaacga agagaaaaaa atcctgaaat tcagggttga ctctgaaaga
    14821 ggaaagcgta atatacgcca cctcgcgaca gtga
//
LOCUS       DACAPB010000114        14666 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.114, whole genome shotgun sequence.
ACCESSION   DACAPB010000114 DACAPB010000000
VERSION     DACAPB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.114"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..530
                     /locus_tag="IT904_003064"
     CDS             <1..530
                     /locus_tag="IT904_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAP3192809.1"
                     /translation="NWLISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLL
                     ERVDIIPTSMVATMAAAESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSS
                     VKESVSAYVTNLNTHPAYSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYL
                     FAMYQDNQRLIAAHM"
     gene            complement(726..1904)
                     /locus_tag="IT904_003065"
     CDS             complement(726..1904)
                     /locus_tag="IT904_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAP3192810.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(1982..3163)
                     /gene="xylH"
                     /locus_tag="IT904_003066"
     CDS             complement(1982..3163)
                     /gene="xylH"
                     /locus_tag="IT904_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAP3192811.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(3141..4682)
                     /gene="xylG"
                     /locus_tag="IT904_003067"
     CDS             complement(3141..4682)
                     /gene="xylG"
                     /locus_tag="IT904_003067"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAP3192812.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(4760..5752)
                     /gene="xylF"
                     /locus_tag="IT904_003068"
     CDS             complement(4760..5752)
                     /gene="xylF"
                     /locus_tag="IT904_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3192813.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6118..7440
                     /gene="xylA"
                     /locus_tag="IT904_003069"
     CDS             6118..7440
                     /gene="xylA"
                     /locus_tag="IT904_003069"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAP3192814.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            7512..8966
                     /gene="xylB"
                     /locus_tag="IT904_003070"
     CDS             7512..8966
                     /gene="xylB"
                     /locus_tag="IT904_003070"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAP3192815.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            9135..9476
                     /locus_tag="IT904_003071"
     CDS             9135..9476
                     /locus_tag="IT904_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192816.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINTYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            9522..9959
                     /locus_tag="IT904_003072"
     CDS             9522..9959
                     /locus_tag="IT904_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAP3192817.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(10001..10996)
                     /locus_tag="IT904_003073"
     CDS             complement(10001..10996)
                     /locus_tag="IT904_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP3192818.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAVLFVTGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLIKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            11171..11470
                     /locus_tag="IT904_003074"
     CDS             11171..11470
                     /locus_tag="IT904_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192819.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPPPEQKAQRVKVDPIRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            11565..12476
                     /gene="glyQ"
                     /locus_tag="IT904_003075"
     CDS             11565..12476
                     /gene="glyQ"
                     /locus_tag="IT904_003075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP3192820.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            12486..14555
                     /gene="glyS"
                     /locus_tag="IT904_003076"
     CDS             12486..14555
                     /gene="glyS"
                     /locus_tag="IT904_003076"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP3192821.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         3698 a         3563 c         3705 g         3700 t
ORIGIN      
        1 gtaactggct catttcaaaa cagtatcagg gccaatggtc acctgctgag cgtgcgcgtc
       61 tgaaagacat cgccaaacgc tacaaggtga agtggtccgg taatacgcga aaaatcccgt
      121 ggaatacctt gcttgaacgc gtagacatta tccccaccag tatggtggcg acgatggctg
      181 cagcagaaag cggttgggga acgtcgaagc tggcgcgcaa caacaacaac ctgttcggca
      241 tgaaatgcat gaaaggacgt tgtaccaatg cgccaggtaa agtgaaaggg tactcacagt
      301 ttagttctgt caaagaatcg gtgagcgcct atgtcactaa cctgaatacg cacccggctt
      361 actcttcgtt ccgtaaatcg cgtgcgcagc tgcgtaaagc ggatcaggaa gtgactgcca
      421 cagcgatgat tcacaagctg aagggctact cgaccaaagg gaagagttat aacaactacc
      481 tgttcgcaat gtaccaggat aaccaacggt taatcgcggc gcatatgtaa ttgcatttcc
      541 ttgagcctta tccgacttgt cagtcggata aggcttttta ctttgtctca ggcagttgag
      601 ctacgagcct gaagcgttgt tggtgcgttt tatcatgcct ggcgggtagg tcggataagg
      661 cgttcacgcc gcatccgaca accacgcagc gttacctgat gtgacgccga caattctcat
      721 catcgctaca acatgacctc gctatttaca tcgcgatact cttttggcgt cgtgtcatat
      781 gcttttttaa aaacagagta gaaatattgc agcgatggat aaccgcacat ttgcgatatc
      841 tcattgatcg acaaggtggt tgaaatcagc agactgcgcg ctttctccag cttctcggca
      901 tgaatcatgg catggatggt ttcacccacc tcttctttaa aacgcttctc aagattggag
      961 cgcgagatcc cgaccgcatc cagtacctga tccactttaa tccctttaca ggcgtgatta
     1021 cgaatgtaat gcatggcctg aataacggcg ggatcggtca gcgagcgata atctgttgag
     1081 cgccgttcaa tgacgcgaac tggtgggacc aaaattcgct gtagcggcat ttcttcttta
     1141 tctaataatc gatgcaacag ttttgccgcc tgatagccca tttgccgcgc gccctgagcg
     1201 accgaagaaa gggcgacacg cgacagatag cgggtcagtt cttcgttatc gatgccaatc
     1261 acgcataatt tttccggtac gggaatatgt agatgttcac atacttgcag aatatgccgc
     1321 gctcgggcgt cagtaacggc aataatcccg gtttgcggtg gtagcgtttg tagccagtct
     1381 gccagccgat tttgcgcgtg ttgccagttc tctggcgcgg tttctaaccc ctgataaacc
     1441 actccgcgat acttttcttc ggcgacaagc tgacgaaatg catattcgcg ctcagtggcc
     1501 caacgtttgc cgcttgattc cggaagacca taaaaagcaa agcggttaac gcctttctct
     1561 tttaaatgca aaaatgcgct ttcaaccagc gcatagttat cggtggcaat gtaatgaacg
     1621 ggtgggtaac tttctgcaag gtgatacgag ccgccaaccc caacaatggg gacgtcgaca
     1681 tcagccagcg cttgctcgat ctgtttgtcg tcgaagtcgg caatgacgcc atctcctaac
     1741 cagtccttga ttttatcaat gcgggcgcgg aaatcttctt caatgaaaat atcccattcc
     1801 gattgtgacg cctgtaaata ttcccctacg ccttctacta cctgccggtc ataggcttta
     1861 ttggcattga acagtaatgt gatgcggtga cgtttagtaa acatggttct tttcctgctg
     1921 aatcatgcaa aaactcaaaa ccggtaatac gtaaccggct ttgagaaaat ttttatcaaa
     1981 atcaagaacg gcgtttggtt gcggagtcca tccatactgc cagcaacaga atcgcacctt
     2041 taacgatata ctgccagaag gtcggtacat ccatcatact catgccgtta tccagtgaag
     2101 ccatgataaa tgcccccatt actgctccgg caacgcttcc cacaccgcca gccaggctgg
     2161 tgccgccaat cacgcatgct gcaattgcgt ccagttcggc gatatttccc gcagaaggtg
     2221 aaccagcgcc aagtcgagaa ctaaggatta atccggcgat ggctaccatt aatccgttaa
     2281 tcgcgaacac ggcaagtttg gtgcgttcaa cgttaatccc ggagagacgt gctgcttcca
     2341 gattgccgcc gatggcataa atgcgtcgtc caaatgccgt ccgcgttgcc ataaacattc
     2401 cgccgagtaa cagcaacgtc agcagcagaa caggagtggg aacgccacgg taatcattca
     2461 acagccagat tgcgcctaat acgatgatag cggttaaagc ctggcgaccg actactgcgg
     2521 tagaggccgg agactgcaaa cccaaagcct gacggcgcat tcttccgcgc cattgccaac
     2581 caacaaaagc cattaagcca agcgcgccaa tgatgaagcc agtgctggca ggtagatagc
     2641 tttgcccaat ttgtgacatc gcggcgctgg tgggggaaac agtcgtgccg ttggtgatgc
     2701 caatgagtat gccgcgaaat gccaacatgc ccgcgagggt gacaataaat gaagggactt
     2761 tgcggtacgc gacccaccat ccgttccagg caccgagaag cagtcccaga accaacgtca
     2821 caatgatggt aagtggcaaa ggccagccta accagacgtc acaaatcgcc gcgacgccac
     2881 ctaatagccc catcattgag ccgacggaaa ggtcgatttc agcagaaatt atgacgaaca
     2941 ccattcctac cgcgaggatg ccggtaatcg cggtctggcg taacaggttg gagacgttac
     3001 gggcgcttaa gtaggcacca tcggtggtcc aggtaaagaa cagcatgatt gcgatgatag
     3061 ctgcaatcat cacgaagacc tgcaaattca gtgatttcag cccggagaag ccaccggatg
     3121 tcggtacggc caatttcact tcagacggat tgcttttcga catgatgttc gctcctcaat
     3181 gcggcttcca tcacctgctc ctgagtcagg ttatgattta tcaggttggc ttttagtttc
     3241 ccttcatgca tcaccagtac acgatcgcta aggccgagca cttcaggtaa ttcggaagag
     3301 atgacaataa cggcaatacc ctgctggacg agttggttaa ttaatttgta gatctcgtat
     3361 ttcgcgccaa tatcgatacc cctggtgggt tcatcaagaa tgagaatgcg cgggttaagt
     3421 aacagacagc gagcgaggat cgctttttgc tgattgccgc cgctcaaacg tccaatagca
     3481 aggtcggggg acgacgtttt aactttgagt tgctggattg attccagaat acatttttgc
     3541 tctgccgcgt catcaagctg gctaatgcca ccggtaaatt tattgagtgc ggcgagggta
     3601 atatttttac caaccgccat taccggaacg atgccgtcgc gctttctgtc ttcgggtacc
     3661 atcgcaatcc cctgggcgat ggcttgctga cagttacgaa tatctacctg tttgccatca
     3721 atataaattt ttccttccca ttgtccgggc cacacgccaa acaggcactg aatggtctcg
     3781 gtacgtccgg caccaacgag tccggcaata cccagtattt cgccacgttt cagggaaaac
     3841 gagacatcat taactcgttt aatatgacga ttgaccggat gccatgccgt cagatgttca
     3901 atacgtaata tttcatctcc ggtggtatgt ggttcattag ggtaaagcgc ggttaactct
     3961 cgcccgacca tcatggtgat aatatcgtct tcactcattc cggcagcatc acgcgtacca
     4021 atgtgctgtc cgtcgcgaat aacgcaaatc gtatcggaaa tcgctttgac ttcgttgagt
     4081 ttgtgcgaaa tataaataca ggcgataccg tgctgttgta gatcgcgaat aatatccagt
     4141 aaaaccgacg tttcctgctc agttaatgag gctgtcggtt catcgagaat taacaagcgc
     4201 acctgtttat taagtgcctt ggcaatttca accagttgtt gttgcccaag ccctaaatcg
     4261 ccaacgcggg tatcaggtga aatggataaa ctgacctgtg cgagcagctt ctgacagcgt
     4321 agcgtcatca ggtcataatc cataatgcca ttgtgggtta tttcgttacc caggaagata
     4381 ttttccagca cggtcaattc tttcaccagg gccaattcct gatgaatgat ggcgatacct
     4441 ttgcgttcgg tatcgcggat gtgactcgcc tgaatctctt ctcccgcaaa aataatttcg
     4501 ccttcgtagg agccatgggg ataaatacca cacagcactt tcatcagcgt tgatttacca
     4561 gacccatttt ccccacaaag tgagacgatt tcgccagcat tcaaccgcaa gcagacgtta
     4621 tcaatcgcct tcacactgcc gaaggttttg gtaatgttct tcatttcaag tagataaggc
     4681 ataacgactc cacctaagcc aattcattca cgcggcatgg agagaaatca cgcccccgct
     4741 ccgcgccggg gcgtaacgct tacagctcgc tctctttgtg gaatccgtct ttaattaccg
     4801 tatctttgat gttgttttta ttcacatcga tcggtgtcag gaggcgggag gggacatctt
     4861 tcaggccatt attcagtgag gtatctgctt ttggctcctg accattgccc aattcaacgg
     4921 caatttctgc ggcagtattt gccaacaacg taataggttt atacaccgtc atagtttgcg
     4981 taccggcagc aatacgttta atacctgcga gatccgcatc ctggccggag attgctactt
     5041 tccctgataa accttgcgcg cttaatgcct gaattgcccc acctgcggtg gcatcgtttg
     5101 aggcaactac agcatcaatt ttgttattat tggcggttag cgcgttttcc ataattttca
     5161 atgcgttttc cggtaaccag ccatcaaccc attggtcacc aacgacttta atttttccgg
     5221 aatcaacgta aggttttaac actttcattt gtccggcgcg gaacagcttg gcgttgttat
     5281 ctaccggcga gccgcccatc aggaagtaat taccttgcgg aacaatatcg accagggctt
     5341 ttgcctgcag ttcaccgact ttttcgttat cgaaagaaat ataaaaatcg atatccgcat
     5401 cgttaatcat acggtcgtaa gctaatactt taatgccttc ttgtttggct tcttttacaa
     5461 cgttacttaa tacctgaccg ttatacggaa taatgacaag aacatcgaca ccccggttta
     5521 tcatgttttc aatctgcgac atttgtgttt cttcattgcc atttgcagac tgtacaaata
     5581 ctttcgcgcc gagagattct gcctttttca caaagatatc tcgatctttt tgccagcgtt
     5641 caagacggag atcatcaatc gccataccta ttttgacttc tttggcgtgt gcagcaacgt
     5701 tggtaagcag gagtgaggtg caaagggtga gtagaatgtt ctttattttc atggtgtagg
     5761 gccttctgta gttagaggac agttttaata agtaacaatc accgcgataa acgtaaccaa
     5821 tttttagcaa ctaaacaggg gaaaacaatt acagattttt atctttcgat tacgattttt
     5881 ggtttatttc ttgatttatg accgagatct tacttttgtt gcgcaattgt acttattgca
     5941 tttttctctt cgaggaatta cccagtttca tcattccatt ttattttgcg agcgagcgca
     6001 cacttgtgaa ttatctcaat agcagtgtga aataacataa ttgagcaact gaaagggagt
     6061 gcccaatatt acgacatcat ccatcacccg cggcattacc tgattatgga gttcaatatg
     6121 caagcctatt ttgaccagct cgatcgcgtt cgttatgaag gctcaaaatc ctcaaacccg
     6181 ttagcattcc gtcactacaa tcccgacgaa ctggtgttgg gtaagcgtat ggaagagcac
     6241 ttgcgttttg ccgcctgcta ctggcacacc ttctgctgga acggggcgga tatgtttggt
     6301 gtgggggcgt ttaatcgtcc gtggcagcag cctggtgagg cactggcgtt ggcgaagcgt
     6361 aaagcagatg tcgcatttga gtttttccac aagttacatg tgccatttta ttgcttccac
     6421 gatgtggatg tttcccctga gggcgcgtcg ttaaaagagt acatcaataa ttttgcgcaa
     6481 atggttgatg tcctggcagg caagcaagaa gagagcggcg tgaagctgct gtggggaacc
     6541 gccaactgct ttacaaaccc tcgctacggc gcgggtgcgg cgacgaaccc agatcctgaa
     6601 gtcttcagct gggcggcaac gcaagttgtt acagcgatgg aagcaaccca taaattgggc
     6661 ggtgaaaact atgtcctgtg gggcggtcgt gaaggttacg aaacgctgtt aaataccgac
     6721 ttgcgtcagg agcgtgaaca actgggccgc tttatgcaga tggtggttga gcataaacat
     6781 aaaatcggtt tccagggcac gttgcttatc gaaccgaaac cgcaagaacc gaccaaacat
     6841 caatatgatt acgatgccgc gacggtctat ggcttcctga aacagtttgg tctggaaaaa
     6901 gagattaaac tgaacattga agctaaccac gcgacgctgg caggtcactc tttccatcat
     6961 gaaatagcca ccgccattgc gcttggcctg ttcggttctg tcgacgccaa ccgtggcgat
     7021 gcgcaactgg gctgggacac cgaccagttc ccgaacagtg tggaagagaa tgcgctggtg
     7081 atgtatgaaa ttctcaaagc aggcggtttc accaccggtg gtctgaactt cgatgccaaa
     7141 gtacgtcgtc aaagtactga taaatatgat ctgttttacg gtcatatcgg cgcgatggat
     7201 acgatggcac tggcgctgaa aattgcagcg cgcatgattg aagatggcga gctggataaa
     7261 cgcatcgcgc agcgttattc cggctggaat agcgaattgg gccagcaaat cctgaaaggc
     7321 caaatgtcac tggcagattt agccaaatat gctcaggaac ataatttgtc tccggtgcat
     7381 cagagtggtc gccaggagca actggaaaat ctggtaaatc attatctgtt cgacaaataa
     7441 cggctaactg tgcagtccgt tggcccggtt atcggtagcg ataccgggca tttttttaag
     7501 gaacgatcga tatgtatatc gggatagatc ttggcacctc gggcgtaaaa gttattttgc
     7561 tcaacgagca gggtgaggtg gttgcttcgc aaacggaaaa gctgaccgtt tcgcgcccgc
     7621 atccactctg gtcggaacaa gacccggaac agtggtggca ggcaactgat cgcgcaatga
     7681 aagctctggg cgatcagcat tctctgcagg acgttaaagc attgggtatt gccggccaga
     7741 tgcatggagc aaccttactg gatgctcaac aacgggtatt gcgccctgcc attttgtgga
     7801 acgacgggcg ctgtgcgcaa gagtgcactt tgctggaagc gagagttccg caatcacgag
     7861 tgattaccgg caacctgatg atgcccggat ttactgcgcc taaattgcta tgggttcagc
     7921 ggcatgagcc ggagatattc cgtcaaatcg acaaagtatt attaccgaaa gattacttgc
     7981 gtctgcgtat gacgggggag tttgccagcg atatgtctga cgcagctggc accatgtggc
     8041 tggatgtcgc aaagcgtgac tggagtgacg tcatgctgca ggcttgcgac ttatctcgtg
     8101 accagatgcc cgcattatac gaaggcagcg aaattactgg tgctttgtta cctgaagttg
     8161 cgaaagcgtg gggtatggcg acggtgccag ttgtcgcagg cggtggcgac aatgcagctg
     8221 gtgcagttgg tgtgggaatg gttgatgcta atcaggcaat gttatcgctg gggacgtcgg
     8281 gggtctattt tgctgtcagc gaagggttct taagcaagcc agaaagcgcc gtacatagct
     8341 tttgccatgc gctaccgcaa cgttggcatt taatgtctgt gatgctgagt gcagcgtcgt
     8401 gtctggattg ggccgcgaaa ttaaccggcc tgagcaatgt cccagcttta atcgctgcag
     8461 ctcaacaggc tgatgaaagt gccgagccag tttggtttct gccttatctt tccggcgagc
     8521 gtacgccaca caataatccc caggcgaagg gggttttctt tggtttgact catcaacatg
     8581 gccccaatga actggcgcga gcagtgctgg aaggcgtggg ttatgcgctg gcagatggca
     8641 tggatgtcgt gcatgcctgc ggtattaaac cgcaaagtgt tacgttgatt gggggcgggg
     8701 cgcgtagtga gtactggcgt cagatgctgg cggatatcag cggtcagcag ctcgattacc
     8761 gtacgggggg ggatgtgggg ccagcactgg gcgcagcaag gctggcgcag atcgcggcga
     8821 atccagagaa atcgctcatt gaattgttgc cgcaactacc gttagaacag tcgcatctac
     8881 cagatgcgca gcgttatgcc gcttatcagc cacgacgaga aacgttccgt cgcctctatc
     8941 agcaacttct gccattaatg gcgtaaacgt tatcccctgc ctgaccgggt gggggataat
     9001 tcacatctat atatctcagt aattaattaa tatttagtat gaatttattc tgaaaatcat
     9061 ttgttaatgg catttttcag ttttgtcttt cgttggttac tcgtaatgta tcgctggtag
     9121 atatggagat cgttatgaaa acctcaaaga ctgtggcaaa actattattt gttgtcgggg
     9181 cgctggttta tctggttggg ctatggatct catgcccatt gttaagtgga aaaggctatt
     9241 ttcttggcgt gttaatgaca gcaacttttg gcaactatgc atatcttcgc gcagaaaaac
     9301 tcgggcaact ggatgatttt tttacccata tctgccagtt agttgcgtta atcactatcg
     9361 gtctcttgtt tatcggtgtt ttaaacgcac ctatcaatac ttatgaaatg gtgatctatc
     9421 ccatcgcctt ttttgtctgc ttgtttggtc aaatgcgttt gtttcgctcg gcatgagcaa
     9481 cataaagctc ttacatattc aggaatgaaa ggaatactgt gatggacaac aaaatatcaa
     9541 cctattcacc agcctttagt attgtgtcat ggatagctct cgttggtggt atcgttacct
     9601 atctgttagg gctatggaat gcagagatgc agttaaatga aaaaggatat tattttgccg
     9661 tactggtatt aggactgttt tctgcggcgt cttatcaaaa gaccgttcgg gacaagtatg
     9721 aaggcatacc gaccacttcc atttattata tgacctgcct gactgtcttt attatctctg
     9781 ttgcattact gatggtaggt ctgtggaatg cgacattatt actcagcgaa aaaggttttt
     9841 atggactggc tttcttctta agcttgtttg gtgcagtagc ggtgcagaaa aatattcgtg
     9901 atgccggaat aaacccacca aaagaaacac aggttaccca ggaagaatac agcgaataac
     9961 tcacgtaagc ccggtcagtc caatgtgacc gggcttttac ttaactcact aatctgtttc
    10021 tgtcgattcg ttgtaccagc atagaaagta acaaactcgc tgccaacgtc gcgcaaaaga
    10081 tccaaataat atccagtatt ggccaatttt taagctcaat tccccgggtg cgcagcgcat
    10141 ggataatcaa ggcgtggaat ccgtatatac ccaacgaatg gcgtgagatt aagccaagtc
    10201 cgcgaatggt acgcgtatcc agcgtgtttt taatcagagt caatagcgcg attgcgcaga
    10261 taaaaaccat cggcccacag taaagatacc aggtatcggc aaaatttccg cgccactgca
    10321 attcatataa tgtcccgcga gagataataa aaacccccgt cacaaacagt actgcgctca
    10381 cccacgacag tgctttatgc tgcgtgtcca tcatccctat agcgcggccc aacatgccat
    10441 acagaatgta gtaaaaagta tcgccattga tatataagtt aattggcagc cattcaaaac
    10501 cgtcgatttt ctgcggcacg gtgtttgggt tagcgatgat accaatcact accattagta
    10561 ccagcaacat ttttccgccg acgttcttca cctgaatcag cggtgaaacc agataaatca
    10621 ccgcaatcgc gaagaaaaac cataagtggt aaaacactgg cttttgcagc aggtttttca
    10681 gcgctaactc cacattgatg gaggtaaata gtgcaatgta gagcagcgcg attgcgctat
    10741 aaaaaaacag acataagccg atacgcaaga aatggcgcgg ctgggcgctg cgttcgccaa
    10801 aaaagagata gccggatatc atgaaaaata gcggcacgct gacacgagag gcagaattca
    10861 gaacattggc gatatcccag gtgacggggc taacactatg agcattggtc acataccagg
    10921 tagtggtgtg aatcatcacc accattaaac acgctatccc tcgcaggtta tcaatccagt
    10981 aaattttggg ctgcatctgt gtctctgtat ctggttaaaa aaagtctgac cgataaatca
    11041 ttcagttggc acactggaat aatctgagtt ttatcactac agcttataga ggcttaagga
    11101 aattcgtaag atatcagcca ctataccgat ataaataata agactcacct gcaaaccaga
    11161 cggtaattta atgatgatga acgctttctt tccggcaatg gcgcttatgg tgctagtggg
    11221 ttgttctaca ccaccaccag aacagaaagc tcaaagggtg aaagttgatc ccattcggtc
    11281 attgaatatg gaagcgttat gcaaggatca ggcggcaaaa cgttataaca ccggcgagca
    11341 aaaaatcgac gtcaccgcct tcgaacagtt ccagggaagc tatgaaatgc gcggttatac
    11401 cttccgtaaa gagcagtttg tctgttcttt tgacgcggat ggccattttt tgcatctttc
    11461 catgcgttaa gccctgcttt ttcccgtttc gtactgtata tcttccatcc agcgggtata
    11521 ctgatccctt cctttaaatc cacacgtatc cagcacgaaa taatatgcaa aagtttgata
    11581 ccaggacctt ccagggcttg atcctgacct tacaggatta ctgggctcgc cagggctgca
    11641 ccattgttca accattggac atggaagtcg gcgcgggaac ctctcaccca atgacctgtc
    11701 tgcgcgcgct ggggccagaa ccgatggcgg ctgcttatgt tcagccttct cgtcgcccga
    11761 ccgatggtcg ctacggcgaa aaccccaacc gtttacagca ctactatcag ttccaggtgg
    11821 tcattaagcc atcgccggac aatattcagg agctgtacct cggttctctg aaagagctgg
    11881 gcatggaccc gactattcac gacatccgtt tcgtggaaga taactgggaa aacccgacgc
    11941 tgggtgcctg gggactgggc tgggaagtgt ggctgaacgg catggaagtg acgcagttca
    12001 cttacttcca gcaggttggt ggtctggagt gtaaaccggt taccggcgag atcacctacg
    12061 gtctggaacg tctggctatg tacattcagg gcgtagacag cgtttacgac ctggtctgga
    12121 gcgacggccc gctgggtaaa accacctacg gcgacgtgtt ccatcagaac gaagtggagc
    12181 agtccactta caacttcgaa tacgcggatg tggacttcct gttcacctgc ttcgagcagt
    12241 acgagaaaga agcgcagcag ctgctggcgc tggaaaatcc gctgccgctg ccagcctacg
    12301 agcgtattct gaaagccgcc cacagcttca acctgctgga tgcgcgtaaa gccatctccg
    12361 tcaccgagcg tcagcgctac attctgcgca ttcgcaccct gaccaaagca gtggcagaag
    12421 catactacgc ttcccgtgaa gccctcggct tcccgatgtg caacaaagat aagtaagagg
    12481 cggctatgtc tgagaaaact tttctggtgg aaatcggcac tgaagagctg ccaccaaaag
    12541 cactgcgcag cctggctgag tcctttgctg cgaactttac tgcggagctg gataacgctg
    12601 gcctcgcaca cggcaccgtt caatggtttg ctgctccgcg tcgtctggcg ctgaaagtag
    12661 ctaacctggc ggaagcgcaa ccggatcgtg aaatcgaaaa acgcggcccg gcgattgccc
    12721 aggcgttcga cgctgaaggc aaaccgagca aagcggcaga aggttgggcg cgtggttgcg
    12781 gtattaccgt tgaccaggct gagcgtctga ctaccgataa aggcgaatgg ctgctgtatc
    12841 gcgcccatgt gaagggcgaa agcaccgaag cactgctgcc gaatatggtt gcgacttctc
    12901 tggcgaaact gccgatcccg aaactgatgc gttggggcgc aagcgacgtg cacttcgtgc
    12961 gtccggtgca caccgtgacc ctgctgctgg gcgacaaagt cattccggca accattctgg
    13021 gcattcagtc cgatcgcgtg attcgcggcc accgctttat gggcgagccg gaattcacca
    13081 tcgataacgc cgatcagtat ccggaaattc tgcgtgagcg tgggaaagtc atcgccgatt
    13141 acgaagaacg taaggcgaag attaaagccg atgccgaaga agcagcgcgt aagattggcg
    13201 gtaacgctga cttaagcgaa agcctgctgg aagaagtggc ttcgctggtg gagtggccgg
    13261 tcgttctgac cgcaaaattc gaagagaaat tcctcgcggt gccggctgaa gcgctggttt
    13321 acaccatgaa aggtgaccag aaatacttcc cggtgtatgc gaacgacggc aaactgctgc
    13381 cgaactttat cttcgttgcc aacatcgaat cgaaagatcc gcagcagatt atctccggta
    13441 acgagaaagt cgttcgtccg cgtctggcgg atgccgagtt cttcttcaac accgaccgta
    13501 aaaaacgtct tgaagataac ctgccgcgcc tgcaaaccgt gttgttccag caacagttgg
    13561 ggacgctgcg cgacaaaact gaccgcatcc aggcgctggc tggctggatt gctgaacaga
    13621 ttggcgctga cgttaaccac gctacccgtg cgggtctgct gtctaagtgc gacctgatga
    13681 ccaacatggt cttcgagttc accgacaccc agggcgttat ggggatgcac tatgcgcgtc
    13741 acgatggcga agcggaagat gtcgcggtgg cgctgaatga gcagtatcag ccgcgttttg
    13801 ctggtgatga cctgccgtcc aacccagtag cttgtgcgct ggcgattgct gacaagatgg
    13861 ataccctggc gggtatcttc ggtatcggtc agcatccgaa aggcgacaaa gacccgtttg
    13921 cgctgcgtcg tgccgcgctt ggcgtgctgc gaattatcgt tgagaagaac ctcaaccttg
    13981 atctgcaaac gctgaccgaa gaagcggtgc gtctgtatgg cgataagctg actaatgcca
    14041 acgtagttga tgatgttatc gactttatgc tcggtcgctt ccgcgcctgg tatcaggacg
    14101 aaggttatac cgttgacacc atccaggcgg tactggcgcg tcgtccgact cgtccggctg
    14161 atttcgatgc ccgtatgaaa gcggtatcgc atttccgtac cctggatgca gctgctgcac
    14221 tggcggcggc gaacaaacgt gtatctaaca ttctggcgaa atctgacgaa gtgctgagcg
    14281 accgcgtgaa tgcctctacc ctgaaagagc cggaagaaat taaactggcg atgcaggttg
    14341 tggtgctacg tgacaagctg gagccgtact ttacggaagg tcgttaccag gatgcgctgg
    14401 tcgaactggc tgagctgcgt gaaccggttg atgctttctt cgataaagtg atggtcatgg
    14461 ttgatgacaa agaattgcgt atcaaccgtc tgaccatgct ggagaaactg cgcgaactgt
    14521 tcctgcgcgt tgcggatatt tcgctgttgc aataataacg ccgttattaa atagcctgcc
    14581 atctggcagg ctttttttat cgctaaataa tacagcaacc tttaataatc ttctgctgaa
    14641 taaagattat ctcatatatt aatttt
//
LOCUS       DACAPB010000115        14455 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.115, whole genome shotgun sequence.
ACCESSION   DACAPB010000115 DACAPB010000000
VERSION     DACAPB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.115"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(38..1534)
                     /locus_tag="IT904_003077"
     CDS             complement(38..1534)
                     /locus_tag="IT904_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3192822.1"
                     /translation="MTISFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVATIASSIKDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(1518..1688)
                     /locus_tag="IT904_003078"
     CDS             complement(1518..1688)
                     /locus_tag="IT904_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAP3192823.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(1697..2257)
                     /locus_tag="IT904_003079"
     CDS             complement(1697..2257)
                     /locus_tag="IT904_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192824.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKAILG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            complement(2254..2760)
                     /locus_tag="IT904_003080"
     CDS             complement(2254..2760)
                     /locus_tag="IT904_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192825.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRRR"
     gene            complement(2735..3145)
                     /locus_tag="IT904_003081"
     CDS             complement(2735..3145)
                     /locus_tag="IT904_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP3192826.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVESQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGEYRQSHGGNNDDFLFAR"
     gene            complement(3142..3465)
                     /locus_tag="IT904_003082"
     CDS             complement(3142..3465)
                     /locus_tag="IT904_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP3192827.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(3468..3668)
                     /locus_tag="IT904_003083"
     CDS             complement(3468..3668)
                     /locus_tag="IT904_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192828.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            complement(3718..4923)
                     /locus_tag="IT904_003084"
     CDS             complement(3718..4923)
                     /locus_tag="IT904_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP3192829.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVEAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGDATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(4938..5588)
                     /locus_tag="IT904_003085"
     CDS             complement(4938..5588)
                     /locus_tag="IT904_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP3192830.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRVWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(5566..6807)
                     /locus_tag="IT904_003086"
     CDS             complement(5566..6807)
                     /locus_tag="IT904_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP3192831.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATDERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(6807..6989)
                     /locus_tag="IT904_003087"
     CDS             complement(6807..6989)
                     /locus_tag="IT904_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192832.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(7001..8497)
                     /locus_tag="IT904_003088"
     CDS             complement(7001..8497)
                     /locus_tag="IT904_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP3192833.1"
                     /translation="MPITLEPWQLFVICCAFGWVNKGSRLRRFREVYTEIPRKNGKSA
                     ISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIEVNAS
                     NMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQPLMWA
                     ITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIYTVDEGDDWTDPQVLEKANPNI
                     GVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLE
                     QFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNED
                     RRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHD
                     LADEDLNPVTIVQNFANMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKNMPGND
                     DLVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            complement(8731..9225)
                     /locus_tag="IT904_003089"
     CDS             complement(8731..9225)
                     /locus_tag="IT904_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP3192834.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGKKKATNPFLK
                     IIES"
     gene            complement(9351..9701)
                     /locus_tag="IT904_003090"
     CDS             complement(9351..9701)
                     /locus_tag="IT904_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP3192835.1"
                     /translation="MPPRIPKSCRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDAIRERVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADCNLQSLC
                     WPCHKAKTARERLK"
     gene            complement(9759..10265)
                     /locus_tag="IT904_003091"
     CDS             complement(9759..10265)
                     /locus_tag="IT904_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192836.1"
                     /translation="MRPDQFFYPDTFTFNSSTYYGQRDSSKGRLYIPIESDLCPFNIG
                     DIIVQKMVDRERLFEVLDYEVQISLEVGCPGYSHLAVLIVKALDVKEKPKQITTHLTF
                     NGAINAGGDFQAGNDNSITKNITIQQLHDAIEHCNDPEVKSLWQKLLENPTFASIASI
                     LAKSALGQ"
     gene            complement(10603..10995)
                     /locus_tag="IT904_003092"
     CDS             complement(10603..10995)
                     /locus_tag="IT904_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAP3192837.1"
                     /translation="MKLRYKLVISAFLLTLFGSLVWSANHYHSKYQREKKRADEAVQN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRTQADIKVAVADDDCAVRPVP
                     AAAADRLRKYANSLREHSEDSTTSQLDF"
     gene            complement(10979..11455)
                     /locus_tag="IT904_003093"
     CDS             complement(10979..11455)
                     /locus_tag="IT904_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAP3192838.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGNIRQAIDRCSNIWASLPGAGYGQYEHKIDSLIAKFKEAGGVVNETSL"
     gene            complement(11459..11794)
                     /locus_tag="IT904_003094"
     CDS             complement(11459..11794)
                     /locus_tag="IT904_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAP3192839.1"
                     /translation="MPHNPNTWPDWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGG
                     GGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVI
                     GNKFGGSKE"
     gene            complement(11931..12224)
                     /locus_tag="IT904_003095"
     CDS             complement(11931..12224)
                     /locus_tag="IT904_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAP3192840.1"
                     /translation="MVVSHDSLNCKSSELLDEFKSHRRYFSVSVSVPYTDVRTHKPVQ
                     FYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGFGSTIKAAMALGRRALGV"
     gene            complement(12503..12736)
                     /locus_tag="IT904_003096"
     CDS             complement(12503..12736)
                     /locus_tag="IT904_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192841.1"
                     /translation="MLQRSNYSLVLNKGIFMKHHEQIEIEAAKVVAELFAGNASPMET
                     FGITWSQTQMLERKNPGVVIKLVPDDGKKLAYC"
     gene            complement(12880..13419)
                     /locus_tag="IT904_003097"
     CDS             complement(12880..13419)
                     /locus_tag="IT904_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192842.1"
                     /translation="MFQAFQTAVFKNVAEYLKGRFPHKSLGLVYADELVLKDMEFLKA
                     NNKLRWDPGLKSRVFMDMMEEHPIKLVVYYRGEPIGFAFGCYCKPKNAVHICWMEKRN
                     DAHEDLDHQMLGIVLDCFSAYARFLNLQGESIDSIALVSPVDGAIRYYTESGFEYIAD
                     YERGGCAMVLRSVLPNQSV"
     gene            complement(13633..14385)
                     /locus_tag="IT904_003098"
     CDS             complement(13633..14385)
                     /locus_tag="IT904_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP3192843.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHDVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
BASE COUNT         3404 a         4066 c         3415 g         3570 t
ORIGIN      
        1 tcaattttga aataacaggt tcccccgata cgggccatta tgcagactcc tctgaatact
       61 gaagacggaa ctggttaacc acggcaaaga cacgcaactg gttaacatag tcaggcggga
      121 acagcgtgtt caggcggttc ggatcgctgg catcacgctc cacaaccagg tactgcttaa
      181 acagttcgta gttttccacg atccccgcac gctcaagctg acggtaggtt gccagcagtt
      241 cccctttgat caccgccggg gtgacaatcg cctgaccggg accaaagcgg gtaccgtcac
      301 tggcaagctt gtgacgcccg tacttactgg taatgacgga tttcagtttg cgcagtacat
      361 acgcgctggt atgcagtgtc tcactgtcga ggtagctgtt atccgcaacc ccgtaagcgt
      421 ttttcctgta cgtggtgaca tcacgctgaa tgcgcagtac cccgctttcg acatacgccg
      481 ttgccacgcc atgagacagc agggtctgtt gttcggtcat cgtgaaccgt ttccccttcg
      541 gcgcaggcag catacccacc agctcaccgg tctgcgtggg acgtgccgga tcgttgcgga
      601 taaacaccgc tgcgcgggcg gtacggcttg ccgccagctc gtcggcaggc gtctgggtct
      661 ctttttcgta tcccgccagg gtaatgtgct gctggttaaa ctggtcacct gcggtcacca
      721 gttctgacag cgtgccgatc tttgccgtat acacatgacc atacagctga cgcgcatagc
      781 tccagcgacc gctggtatcg ttcatctcgg tcaccagcgt gttaacggag gctgtgtcgt
      841 tgaacggcag gccgatataa tcaaacggct catccgccat tgcagccacc gcgccggtga
      901 gaacaggagc gcccgttccg gcggtacccg tcgccacggc aatctgtacg cccgctggca
      961 gcacttcgcc cccaccaaag ccgtagtaat tgaggctgac aggaatttca ttcccgcaaa
     1021 gccccttatg acgcgcggtc agtgtgacca cgcctgccga agatgaggca gtaaacggca
     1081 gggtcggaac ggcattgatg gcatctttga tactgctggc aatcgtcgcg acgttatcgc
     1141 cgttggtcac cggtgcctgc acgcgggtac gtcccacata aacattcacc gtgccggttt
     1201 cggttgccgc gccggtcacc gtcagcgtaa ctgttgccgc cgcgcccgtg gattcaggaa
     1261 cggcaatcac atacagttca ccaaacgggt cggtctggcg ataagcctcg accatacgcg
     1321 ccagctgact tcccgcacca caaatctggc gtgcatagtc tgccgatggc atcagcacca
     1381 gactgttggc aacaatctct gcaccgttat tggcatgacc aatcagcagc gatgctccgc
     1441 tgtcctgtgc agtattcgcc gccgagttat ccatttccgc ataaaaaatc ggaaccagcg
     1501 tattcgacgg aatggtgtta aagcttatcg tcatcggtat tcaccttttt attcacgcgc
     1561 cggatatcac ccgctgcttc acggcgcagc cagtagttgt tctcatcaac atttcgcccc
     1621 tcggcgggca aaaggtcgcc gcgggcaggg tcaggaactg accgcccttt aacaggtttc
     1681 acaaacatga agattctcag gaaggaaggg ttatttcggt gtgatgttcg atatcgccgt
     1741 caggcccgtt accgggatcg agataatcaa catcaatcgc cagcgttcgc agttcatcca
     1801 gactgttcag gtcatcctgc tggcgggtat cgtcttcggt cagctcgctg atgaccgaaa
     1861 aatcgaactg ataaatcagc tcatgacgat tcagatccag cagcgtgccg ccgtcataag
     1921 taatcgggtt accgcacgct tccgggttcc agcccagaat ggccttaaag agcatctgcc
     1981 ggacatcgtc caccacatca tacgaggcaa actgaccgcg ctcatcacgc ccgttactca
     2041 gtatgacaac cacggagaaa ccctctttca gctcctgcca gtagtcggtc tggcttttgt
     2101 tttctcccgg agagtcatca cccggtacca catacgccgc cgggagtctc agctttccga
     2161 cctccggcag atttttgaac tgtgccgcgc ctgccacccg gttttcaaaa tacgggcagc
     2221 gggcacgcag cgcagcaata acaggcgtca gtttcatctg cgtcgtcgct ccggcttcag
     2281 tgatttacgt aattcccgcg ccagaaaata gcgtgtccag ctgcggttct tttcaagcgt
     2341 ttccaccatg aagttattac gtggagccag tcgccagccg ctgccaccgg atgcaccacg
     2401 atgatggctg cgacgacgct ttgcccctcg cctcacgcca tagaacaaaa aagccggata
     2461 aaaatcaccg gtgatacggc ggtttccctc tccattacgc tggttagggg ctatacgtgc
     2521 cataaaacca gggcgatgtt tactggctct gggtaccata tacccaatcg aacgtgccag
     2581 gcgtccggtc tgataaccgg ggttttcacc cggtgccgac cgcgcacggc gcatcaccag
     2641 ccgacgggca tcacgcatat gacgctgacc aatcgtgaca aacgcccgcc ggacacgggc
     2701 gcggttaaag cgcatctccg cgggctgctg aaaatcaacg tgcaaaaagg aagtcgtcat
     2761 tgttgcctcc atgactctgc ctatattcgc ccagctccgt acactccagc agcagaaagc
     2821 gccgcgcccc gttcagatcg cgctgacgtt tcacccggta cacactgtca tcacagacca
     2881 cctcataatc agcagtgatc ccccggcggt agcgaatggt gatgtaatgg gtgatggcgt
     2941 ccccggtctg cgcggtttcc tgccaggtgg tggcactggt ctggataacc ttcgcccatg
     3001 tccggaacgt aaccgggtat tgagactcca caccaaagtt atccgcgggc atatccaccc
     3061 gctggcggat caggacgcgt ttattcagtt caccggggtc cggcagaatg taggttgcgc
     3121 tggtctgcgc ctgacgaatt ttcattgcgg aaagtacctg tacgggccga caagccagcc
     3181 aaaactctgc ggcatgtcga gtttctccac ttccgtaacc gacgagcggt tttcgtaaaa
     3241 atggctgata agcatcagca tccccaggcg aatatcatcc ggcaggtgca gcccgtctgg
     3301 atcgctgtcc ggaatggttt catccggtgc atagagcttc cggttcagat acgtttccgt
     3361 ccgcttttgc gccgcacagg ccagcagttg cagatggcgg tcatcagcat cgaaatcctc
     3421 atccagccgg agttgggctt taatctcttc cattgtcaga agcatactca gccctcttta
     3481 ctggtcgtgg cttttttctc ttttgccgct ttactgcttt ttgcactgat tccgcgctct
     3541 gctaacccgg cctgaagtgc aatctcctgc acccgggcag gaagcgcccc gtcgtcatac
     3601 tcaccggccc gaatgacctc aacacgcata ccgtccggtg accatttcag atcttgtttc
     3661 aggatcatga ttcttcaccc gtcagaacag ggggcgcggt tccgcgcccc tgagtgatta
     3721 cgccgctgca atcttcagca gtttgatggc ctgcgaatcg accagcatcc cgccggtgcg
     3781 cttggtggta taaaaaccga caaacggttt attggtgtac gggtcacgca gaatgcgggt
     3841 gccgatacgg tcaacgatgg tgtaaccccg tttgaagtta ccaaatgcaa tggctttcgc
     3901 atcagcggcg atatccggca tctgttcgtt ttcagcgata ccgtaacccg ccagagagga
     3961 cggctgcccc agctccagcc ccggacgcca cagatagtta ccctcgctgt ctttcagcag
     4021 acggatggca aacaggctat tgttgttcat catgaacttc gcgccggtgc ggtgtgcctt
     4081 acgcagcgtg taaatcagtt tgataatggc gtctgcggtc accgccgtcg cgtcgccgga
     4141 tacaatatgc tgaagtttgc cgaacgcccg gaccttatcg gtttcatccg tggattcata
     4201 cgccaggaac cctttcggct tcttggtacc atcgccggtg gtaaaggcaa tttcttcctg
     4261 ttcagcaaat tcggtcgcca gctcgctgtt gatccaggcc tccacgttga agaaggcatc
     4321 atccagcatt ttctgagtgg cctgcgggtt accgtagatt tcccccatga aaggttcaat
     4381 caggcccagt tttgaggtgg cagtctggga gcgcgcgtca gtctcgccaa cccatccgga
     4441 agccgtgccg cccagattca ccagtttttt gtagtcggaa ccaccaacgg tgatcaccgt
     4501 ggcttcctga cgcatcacca cttcatcttt cagcaaggtc agaatgttgc gatccagcgc
     4561 ttccggcacg gcatagccac cgtcttcatc ggtgcccacc tgtaatgcct tgcgctccag
     4621 atcgcgcaga ccatcttcac ggcctttacg caggaagccc acaaacgctt ctttatgctc
     4681 ggtggccagt ttattttgcg caccacctgc cggacgtttc agctcaagca gctctttttc
     4741 aaggtcgctt ttgaggtttt ccagctcgct gagttttccg ttcagggttt ccacctgccc
     4801 ggcaagtttg cctttttcct gctcaatcgc ctcaacgcgc ttgtcgttct ttgctttgaa
     4861 gtcgtcaaac ttctgctgca gctcctgcgc gacctgttcg acatctttaa tatcaactgc
     4921 catcgtattt ctcctgatta gaagttcaga tttttcagtg cattcagtgc agagcccaca
     4981 tcctcagcgt cgcgcaggga cagtgcgcca tagcccccgg ccatgaatgc tttggcctgg
     5041 gtacgggaga gtccgacatc acgcaggact ctttcgattt ttttctgttc ggggatttcc
     5101 ccgcgggcca gtgcgttctt gacgtcgctg atccgcgcct cgtcgttaga cgggaatgtc
     5161 accagactga cttcccagag gtcgatttct ttcagcagaa aggcttcttt cgtccggtcg
     5221 tattcccagt ccttcaggac gtacccaata gaaaggccgg ttaacgaacc ggccttcatg
     5281 tgtgcatgtg cgcgttttgc caggggatca tcatcaatga gcaaccgccc cctgacgtaa
     5341 agcccgacat cgtcttcctt catttcggtg taaacaccga tgggctcatc catgcggtgc
     5401 tgccagagca gcgcaggtaa cgcttttctg tcactccaca cccgcaggga agcagcaaat
     5461 gccccggaca tcaccacatc atcgtggctg tcctttacac caaagacgga gccataccct
     5521 tcaaactcac cggagtcact gacagatttc agactcagcg gtacatcaag acgttgtttc
     5581 gtctgcattg gcgttatcct tctgcttacc ggctttactg ccatcggagg gtttcgtggt
     5641 catgttcatc ggtgtgagat agacatcacc accgggacgt ggattcatat cttccaggtc
     5701 gcggcagtca ttgggagagt aaattcccca gttgatcccg gtggcgtagg cttcaaaacg
     5761 ggacttcata tccccgcgca gtaacgcccc ggcgttaaat ttggcgtaat aaacgccctg
     5821 cttacttttt cgtaccagtc cggtgttgat ccgctgttcg atgcgggtca gatacggcac
     5881 cagtgaatag ttgataaatc ccagccccag ctcttcgata ttgttgaagg tggcgcgatc
     5941 ggtgttctgc accatgtgca acggcacacg gaacagacga caaatttctt caagctgaaa
     6001 cttgcgggtt tccaggaact ggctgtcctc tgcgttcaac gccatcgact tccagtccag
     6061 ccccatctca aggatcatcg ggcggtgagc attgccaagc ccggtgtgac gctcctcaaa
     6121 atctttcttc aggcgctcat aagcctgatc cgacagcgtc tgctctgtac gcaacacacc
     6181 cgacgtcacc gcgccattgc tgaacagtct ggccccgtgc tcttcggtcg ctgccgccag
     6241 cgatattgcc tcgcgggcat aggcgatggg attcagcccc accagtccgt ccagcgtcag
     6301 cgtacgcaca tgccagatat cctcctggct cagtacatcc gtggagccat ccgggaatgt
     6361 gacctgatag accggttccc agctactgtt aagcttcggt accacacagc cgggatcgac
     6421 gggcagcagt tcagccactt cgccaaatgc tttcactttg taggcgtaaa agtttccccg
     6481 caggcacaga caggtgacca ccagctccca gaactcctgc ggcgtcatat agccattggg
     6541 atgcgtggag atcagcttat gcagacgttc gtcagtggct ctctgcttca ggctgccgtt
     6601 caggtgatac aggttgcagg gcaacatccc gaccgactcc gccagcaccc tgacacagga
     6661 aaaaaccgcc gtcagtcgca tggcccgctg gctgctgatc tgctttccgg tataggtgtc
     6721 gtaggacaac ccgatagcat ccgccagctc tgctggcgtg gtcaccggtg cgtcactttt
     6781 tcgttgaaat aatcccgaaa agaacactat ttacctccgc cgacagacga ctgtgtacgg
     6841 tcgagatatc gcgccaccag ccacgaccag aacagacaca acgccccggc aacaacaaac
     6901 cccgccgggg gataaatcag ccaggcacca tacgccagca aaagcgcccc cagcacgccc
     6961 accagaggcg cgagaatcag catgatcata attacctcag ttaaagcgag cggatcccat
     7021 aggactcaat gtggtcagac agcgtgtctt ctttctcgta cagcatggct ctgccaaccg
     7081 ccataatcag cgcaactgca ccatcgattt tgttttccgc ctgctctttg acgggcttca
     7141 ctaaatcatc gttacctggc atgtttttgc cgaccacatt gccgatacac caggtcatga
     7201 tgggattgcc gtcatgatga aagcgtcccg attcaatcgc tgcctccagc tctttcatcg
     7261 ggtcggacat attggcgaag ttctggacga tagtaacggg attcaggtct tcatcagcaa
     7321 ggtcatgtga cagcccggtc gctccgaaag ggtcgatggg tgactcgctg accgggctga
     7381 ttttgttcgc cgctttggcc tcttcgagga tgtagcgata atccacctct gcaccatcgg
     7441 taacggtcag aacgcccatt tccacccatt tctgaaagcg ttcggctgtc cggcgatctt
     7501 catttttctc gacgctatac accgtgtcat acggtaccca gaaacgcggg gccacactgt
     7561 agtaatgcgt tttaccgtca atctcgcggg tataaagtcg cgccatgctg ttcatatcca
     7621 gcttacgcgc caggtcaaag gccagaatgc acggctgccc ctcgaactgc tcaagagtca
     7681 gtgatttatc ctcgcagctc tgccagctca ccaggttgaa atacgccgaa cgcgccgaca
     7741 cccagatatt gaggtgtttt gttttaaaga cgtttgccag acgggcgtta tttttcgcac
     7801 gctgctgctg acttaacaaa aattcgcgat aaaccgacac gccaatattc ggattggctt
     7861 tttccagcac ctgcgggtcg gtccagtcgt caccttcatc aacggtatag atgatcccga
     7921 acagttcatc gttaggcacc gagccgttga gcatctcgat gacttcccgc cgcttgtcgt
     7981 agcacggccc ctcaatgttg tacccggcgg tggtgatggc ccacatcagt ggctgacgtc
     8041 gcgcccccat cccggtaagc attgtggtat aaagcgcatc ggtggcatgc tcgtgatatt
     8101 catcaaccac ggcacagtgg ggtgatgaac catcacctgg gttgccgatc agcggttcaa
     8161 accgcgcgcc atcctccgga cggttcatgt ttgaggcgtt aacctcaatc ccgaacgctt
     8221 ccgtcagcat gggtgtgcgt ttacacatca gtcgcgccgg gcgaaagact tcccacgcct
     8281 gtttctctgt cgtggcaccg gaatacactt ccgcgccaaa ctcgttatca caggcaaaac
     8341 aatacagggc aacaccggca gagattgccg atttgccgtt cttacggggg atttcggtat
     8401 acacctcccg gaagcggcgc agccgggagc ctttattgac ccagccaaac gcgcagcaga
     8461 tcacaaagag ctgccacggc tccagcgtga tgggcatccg tttaaatgcc cactcaccct
     8521 tggtgtgcgg caacagctga ataaatttgg cggcccgttc agccagatcc ttgtcgaagc
     8581 ggtaacgaaa cgacttactt ttttccgcca tcaggtcatc aagatggcgc tggcaggcct
     8641 gaatcacaaa ctggcaggcc acaatctttc cgcgcacgac atcccgggca tactgattgg
     8701 cagcatttac gttggggtaa gatttccggc tcatgactcg ataattttca gaaacgggtt
     8761 agtggctttc ttcttccccg ccaggccaat cagacgctgg cggctgctgg ggtcgagtcc
     8821 gagcattgcc cccgtactgc tcatctcgga ctcctgttct tttttggcgg tcagctccgg
     8881 atttttgacc atgccgccca ttgcaccggt gatggtgttg ccctgtctgg caatattttt
     8941 cacggcacgt cgccagaact cataggccac gcaccaccgc tcaagcaccg cgaggtcagt
     9001 cacgcacagc agaccctgac cgcagagttc tttagttgtc agttgccaca tgatcgtagc
     9061 gagagggaga tcttcttcag cgaaccactc cggtggctca acacctttga tgggcgtaaa
     9121 aacaggttca tctttgttca gggctcgctt gccggggttt ccggccagcg ccttgcgcgc
     9181 cgttggctta gggcgacgcc cggaacgccc cgccgttcca gccatatgcg gcactcctgg
     9241 ttaaatttca tttttcgcgg gtataaaaaa acgatggggc gggcagtccg gaagacgtca
     9301 ggccgcaggg atttgacccg cccctcccct caggaagttg agaatcatta tcacttcaac
     9361 cgttcacggg ccgtcttcgc cttatgacac ggccagcaca gactctgcag attacagtcg
     9421 gcatcagtgc cgccatgcgc tttagggatg atgtggtcaa cggttttcgc ttcacgcacc
     9481 acaccggcac gcagacataa ctgacataaa cctttgtcac gtttcagtac acgttcacgg
     9541 atagcatccc atttcgaacc gtagccgcgc tgatgacggg attgtcctgg cttgtattgc
     9601 ttccagcctt cgcttttgtg gctttcgcag tagcctgacg ggtcagtagt ggtatggcgg
     9661 cagccgcgaa cgcggcagga ttttgggatt cgtggtggca ttgaagaaaa ccctttgtaa
     9721 taagcataaa aaccccatat tgaatggggc ttgatgaatt actgtcctaa agcacttttt
     9781 gccaatattg aagcaattga agcaaacgtc ggattttcca atagcttttg ccaaaggctt
     9841 ttaacctctg gatcattgca atgctctata gcatcatgta gttgttgaat ggttatattc
     9901 tttgtgatcg aattatcatt accggcttga aaatcaccac cagcattaat agcaccgtta
     9961 aacgtcaaat gggtcgtaat ctgcttcggc ttctctttta catccaatgc cttcactatc
    10021 aataccgcca aatgtgagta gccagggcaa ccaacctcga gactaatttg cacctcgtaa
    10081 tcaagcactt caaatagtct ttccctgtcg accatttttt gcacaatgat atcgccgata
    10141 ttaaatgggc ataagtcact ttcaatagga atatacaatc ggcctttaga tgagtcacgt
    10201 tgaccataat aggtggatga attaaaggta aaagtatctg gatagaaaaa ttgatctggt
    10261 ctcataatag tcccttttgt gttttgtgga tatctccatt aattagacta ttactttatc
    10321 tcttttccca aatacataca ttcaaaatta agagcattat cacagataat ttcgaagcga
    10381 tattgcaatg cctacccatg acctgtattc aacgaatacg tatcagagga ataccttgtt
    10441 cgttatctac atgacgataa aaatatacat caattttgat tatcaatgat tctgatatct
    10501 gacttatccc tattacattg agccaacgcg gataacaaac ttacattcaa atcaagactt
    10561 gcaccatacg tcagtgggtt gggtataaac ggtacaggtg tatcagaagt caagctgact
    10621 ggtagtggag tcttcggaat gctcacgtaa actgttcgcg tacttccgca accggtcagc
    10681 agcggcagca ggcacagggc gtacagcaca atcatcatcc gcaacagcca ctttgatatc
    10741 agcctgggtt ctctgtgact ccagtgcgat ctgctgtttt gcatgctggt tagcctccag
    10801 aactgtattg acgatttgca gtgattgcag gacgttattg gtaatagcgg ttgccgattc
    10861 ggcattttgt acagcctcat cagcacgttt cttttcgcgc tggtatttgc tgtggtagtg
    10921 gttagctgac cagacgagag aaccgaataa agtcaggagg aaagcagaaa taaccagctt
    10981 atagcgaagt ttcattaacc accccgccag cttctttgaa tttggcaatc agactatcga
    11041 tcttgtgttc atactgaccg tagccagcac cgggcaatga agcccaaata ttgctgcaac
    11101 ggtcgattgc ctgacgaata ttgccacggt caatcatcgg taaagcgcca cgctctttaa
    11161 tctgctgcag agctacagcg tcctggcttt ctggagaaaa atctttcagg ccaagctgtt
    11221 tacggtaagc atcccaccag cgtgaaagaa gctggtaacg tccggcggct gttgacttga
    11281 gtttcgggtt tagcgtgaca agtttgcgag ggtgatcgga gtaatcagtg aagagttcac
    11341 caccgacaat aacgtcataa ccgtggttac gtgtcggttg tcgtccgtta tccgttcctt
    11401 ctgaccaagc caccatatca aggaaagctt tacgctggga atttagtacc tgcataaatt
    11461 actccttaga gccaccaaat ttgttaccga ttactcgcat tgcagcccca cgaatagcat
    11521 cgacaccgat cagccccacg ccaccaccaa tggcaacaga aagcgattta ggccatccga
    11581 catactcaag agcggatgca aaggtcagcg tcagagcgcc acagagcaaa atctcgagcg
    11641 tttttcgctt ccagccacca ccaccgccaa aataggcgat gcgcaaacca gccataacga
    11701 tcgacataat cactgccccc agcggtgtgt ctccacgcca ccagctctga aacaactcca
    11761 gccagtccgg ccaggtattt gggttatgag gcatttcgtc atctctcacc tcgcgatatt
    11821 tgcgggtgct gtgttggaaa taaaaaggcc acgcaacgtg gccaccagaa ttatttcccc
    11881 accagttcac ttacctcttt caccgtctga ttaaaccgct ctgactcaag ttaaacacct
    11941 aacgcccgac gccccagcgc cattgctgct tttattgtgg aaccgaatcc cataaagaaa
    12001 tcagcaacca gatcaccagg tcgactactg gcattgatta tttgcctgag catatccgcc
    12061 ggtttctcgc acggatgttt acccgggtag aactgaacgg gcttatgcgt cctgacatcg
    12121 gtataaggca cggagactga tacggagaaa tagcgccggt gagatttaaa ctcatccagc
    12181 aattcagacg atttgcaatt cagtgaatca tgagatacca ccaactggtg gtaggattgt
    12241 ctcaactagt gagctttata catttaagtg cgatgcacag ataataacca cgccaatgga
    12301 atatcacaca caaaattgat agaacactaa caataagcca caaaataatt cacaatttat
    12361 ttttacagtg gataagtagc aaagtagtga atgtgaataa cggctaacta agatgaaaca
    12421 aaaagcccca caatatgtgg ggctccttcg cgatttatag taaaataatc gattctctag
    12481 ctgaaaaaat ctttaaaaat tatcagcaat acgcaagttt tttaccgtca tccggtacta
    12541 gtttgatcac tacaccagga ttcttacgct ctaacatctg agtttgactc catgtaatac
    12601 caaaggtttc cattggagat gcattgccag caaagagttc ggcgacgact ttagctgcct
    12661 caatctctat ctgctcatga tgtttcataa aaatcccctt gttcagcact aagctgtagt
    12721 tgcttctttg aagcataaag gcaacaaagt ttgttacctg aagatgtata aagtaaagcg
    12781 tagtacattt tgtgtgtgct ttgtgtggat ccagattgca tcaactaaaa gaggttttca
    12841 atacttttgt aatgattctg gagaaattaa gaatctaact cacactgatt ggttaggtaa
    12901 aacgctccta agcaccattg cgcaaccacc tcgttcgtaa tcagcaatgt actcaaaacc
    12961 actttcagta tagtatctta ttgccccatc aactggacta actaaagcta tcgaatcaat
    13021 agattcaccc tgaaggttca gaaatcgtgc atatgcagag aagcaatcca aaacgatacc
    13081 aagcatctga tggtctaggt cttcatgtgc atcattacgt ttttccatcc agcaaatatg
    13141 caccgcattt ttcggcttac agtaacaccc aaatgcaaat ccaataggct ccccacgata
    13201 ataaacaact agttttatcg ggtgttcttc catcatatcc ataaacactc gcgactttag
    13261 tccgggatcc caccgcagtt tattgttagc cttgaggaac tccatatctt ttagtaccag
    13321 ctcgtcggca tatacgagcc ctaacgactt atgagggaag cgccctttta aatactctgc
    13381 aacattttta aatactgctg tttggaaggc ttgaaacatc atgtcgatcc agacgagacg
    13441 aaagtttaaa ttctacctac tcgccgaata ccatttcaat gccacacatc taaaattccg
    13501 aaaaaaaacc cgcacatcag cgggttttct actttttatt aaagccggac acacaatgcc
    13561 tatcattgag aaaatattat ccattttttt gaaaaatgca agcatcatgt cgccattttc
    13621 gtcgaaaatc attcatctcg tcactttcct caattgtgtc tcagcatacg cttcttcctg
    13681 ccagcacttt gtaaccagtt tatcaatgac atctgcatat cctttgtacc actgataatc
    13741 cgtcaggtct ggtaccagct tctggacatg aagccgcgcc agtgtggttg gtaaacggct
    13801 aaaccggttt ccattgcaac gcccacaaac cttataaaca ggcgtgccat gaagcctggt
    13861 tcttttttca tccaggacaa tacctttacc cttacaccct ctgcacgctg tgctgacttc
    13921 tcccttacca tgacaatgct gacacagttc cttcacccac tcttccttga taacagattc
    13981 cccgcttctg gagtgtttca ccacctcgcg caatacatca tgaaatccag taccagcaca
    14041 atgctcacag cgagccttac ttgccgcaga cctggaataa tcagcaaagg caaaattcac
    14101 aaggtaagga atgatctgta gccgggtttc ttcactcaat ttgttcaatg tcgggttatc
    14161 cagtgccatc gcgtaattga gcagaccttc aatcgcaaac tgaggatcct gaacaccaac
    14221 ttttgccagg aataaggcaa acccaagcgg tgctttcgac tgcaccatcc cctgcgcagc
    14281 cattacatct gtaattgtta aaccaccaga gcctgtcgcc ggtgcgtcat cgctcaattt
    14341 tggagatttt ggggagtaat atttcggtaa ggcttcaagg ttcatgctcg ttctccactt
    14401 acgccagtac gcctattgcc agcgcacgat cgataaaacg aaatatcagc tccaa
//
LOCUS       DACAPB010000116        14426 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.116, whole genome shotgun sequence.
ACCESSION   DACAPB010000116 DACAPB010000000
VERSION     DACAPB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.116"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..410
                     /locus_tag="IT904_003099"
     CDS             <1..410
                     /locus_tag="IT904_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192844.1"
                     /translation="ELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKV
                     AVTFDSSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQ
                     TLNFFAWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            461..2920
                     /locus_tag="IT904_003100"
                     /pseudo
     CDS             461..2920
                     /locus_tag="IT904_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2935..3666
                     /locus_tag="IT904_003101"
     CDS             2935..3666
                     /locus_tag="IT904_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3192845.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNIREVPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGSYSGSQFYLGYMD
                     DYGALRMTMLNCSGQCHLQAVEAKK"
     gene            3663..4724
                     /locus_tag="IT904_003102"
     CDS             3663..4724
                     /locus_tag="IT904_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3192846.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            4876..5922
                     /locus_tag="IT904_003103"
     CDS             4876..5922
                     /locus_tag="IT904_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP3192847.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(5919..6710)
                     /gene="nei"
                     /locus_tag="IT904_003104"
     CDS             complement(5919..6710)
                     /gene="nei"
                     /locus_tag="IT904_003104"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAP3192848.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6746..7480)
                     /gene="pxpA"
                     /locus_tag="IT904_003105"
     CDS             complement(6746..7480)
                     /gene="pxpA"
                     /locus_tag="IT904_003105"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAP3192849.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7470..8402)
                     /gene="pxpC"
                     /locus_tag="IT904_003106"
     CDS             complement(7470..8402)
                     /gene="pxpC"
                     /locus_tag="IT904_003106"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAP3192850.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDSM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8396..9052)
                     /gene="pxpB"
                     /locus_tag="IT904_003107"
     CDS             complement(8396..9052)
                     /gene="pxpB"
                     /locus_tag="IT904_003107"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAP3192851.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9075..9818)
                     /gene="ybgI"
                     /locus_tag="IT904_003108"
     CDS             complement(9075..9818)
                     /gene="ybgI"
                     /locus_tag="IT904_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAP3192852.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10089..11570
                     /gene="dtpD"
                     /locus_tag="IT904_003109"
     CDS             10089..11570
                     /gene="dtpD"
                     /locus_tag="IT904_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAP3192853.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITILFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFDYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11720..13138)
                     /gene="phrB"
                     /locus_tag="IT904_003110"
     CDS             complement(11720..13138)
                     /gene="phrB"
                     /locus_tag="IT904_003110"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAP3192854.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13135..13644)
                     /locus_tag="IT904_003111"
     CDS             complement(13135..13644)
                     /locus_tag="IT904_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAP3192855.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13795..14426)
                     /locus_tag="IT904_003112"
                     /pseudo
     CDS             complement(13795..14426)
                     /locus_tag="IT904_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3452 a         3869 c         3515 g         3590 t
ORIGIN      
        1 ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc gatgccgttg
       61 ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc cagcaacggt tccggtggca
      121 agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc gcagatccga
      181 ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag
      241 atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct ccgacctcca
      301 gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct acacctgtaa
      361 cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa gtattacttt
      421 ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg tgaatattta
      481 tcgactctct tttgtatcct gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt
      541 caaccttaat gttctcgata aatcgatgcg cgaccgcatt gatatttcat tattaaagga
      601 aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata ataaccaaat
      661 cagtaacggg caaaagatta actggcacaa aaatgacgat aaaaccattc cgtgcatcaa
      721 tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt taccattgat
      781 aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa
      841 tcagcaacta aatatcacca ttccgcaagc ctggctggcg tggcactcag aaaactggac
      901 cccaccctcc acatggaaag aaggtgtcgc cggtatcctg atggattaca acttgtttgc
      961 cagcagctac cgcccacagg acggcagcag cagcactaac ctgaacgcct acggtaccac
     1021 cggaattaac gccggggcgt ggcgcttacg tagtgattat cagttgaatc agactgatag
     1081 cgatgataac catgaacagt caggcggaat atcgcgcacc tatctttttc gtccattacc
     1141 gcaattaggc tctaaattaa ccctcggcga aacggatttt agttccaata ttttcgacgg
     1201 tttttcttat accggcgcgg cactggcaag tgacgagcga atgttgccat gggaactacg
     1261 cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga cgatcagtca
     1321 atcaggccgc gtcatttacc agaaaaaagt cccaccaggc ccatttatca ttgacgacct
     1381 taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg gtcgggtgaa
     1441 caatttccag gtttcggcag catcgacgcc cttcctgact cgtcagggac aggttcgcta
     1501 taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa atgaaacctt
     1561 ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtacg gcggcctgct
     1621 gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata tgctgtggct
     1681 tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc agtaggacga
     1741 gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta acagcacgat
     1801 ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca actatctgga
     1861 tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct tatctgtggg
     1921 ccaaccgatt accccactaa acctcaatct ttacgccaac ctgctacatc aaacctggtg
     1981 gaatgcagac gcctccacga ccgccaacat cacagccggt tttaatgttg atattggtga
     2041 ctggagagat atctctattt cgacgtcatt caatacaacc cattacgaag ataaagatcg
     2101 cgacaaccag atttacctgt cgatttcgct ccccttcggt aatggtggtc gggttggtta
     2161 tgacatgcaa aacagtagcc acagcaccac acaccgcatg tcgtggaacg atacgctgga
     2221 tgaacgtaat agctggggca tgtctgccgg actgcaatcc gaccgtcctg acaatggagc
     2281 ccaggtgagc ggtaactatc agcacctgag ttcagcgggt gagtgggata tttctggtac
     2341 ctatgccgcc aatgattaca gttccgtcag cagcagctgg agcggttctt tcaccgcaac
     2401 ccaatatggt gcagcgtttc atcgccgcag ctccaccaat gaacctcgcc tgatggtcag
     2461 caccgatggc gtggcagata ttccggttca gggcaatctc gactacacca accattttgg
     2521 cattgcggtg gtgccgttga tttccagtta tcagccttcc accgtggcgg tgaacatgaa
     2581 tgacttaccc gacggcgtaa cagttacaga aaacgttatc aaagaaacgt ggattgaagg
     2641 cgcgataggt tacaaatcac tggcttcccg ttccggtaaa gacgttaacg tcatcattcg
     2701 caacgccagc ggtcagtttc ctcccctcgg agcggatatc cgccaggatg acagcggcat
     2761 tagcgtgggg atggttggcg aggaaggaca tgcctggtta agcggagtcg ctgaaaatca
     2821 aaagtttacc gtggtctggg gtgatagcca gcattgctcg ctccatcttc ctgaacatat
     2881 ggaagacacc gcaaatcgcc tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca
     2941 tttatgaaag gactacccct tttgttgttg gttgccagtc tgtgcagcca cgctgcacta
     3001 caacccgatc gcactcgtat tgtgttcaac gctaatgata aagctaccag tctgcgagta
     3061 gataatcgta gcgataaact cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg
     3121 gagaaaagcg acgatcttct ggtcgccctg ccaccgatac aacgtctgga accgaaagca
     3181 acaacgcagg tgcggatagt caagcaagcg tcgaccgcga aattaccagg cgatcgcgaa
     3241 acgctatttt tctacaatat acgtgaagtt cctcccgccc cggaaaaaaa cagcgaccat
     3301 gccgttttgc aagtcgccat ccagagtcgt atcaaggtgt tctggagacc ggctgcatta
     3361 cgcaagaaag caggagaaaa ggtcgaactg cagttacagg tcagccagca gggtaaccaa
     3421 ctgacactga aaaatcctac cgcgtattat ctgaccattg cctacctggg acgaaatgaa
     3481 aaaggcgttc tccccggctt caaaaccgtg atggttgcgc cttttagtac ggtcaacaca
     3541 aacaccggaa gctatagcgg cagccagttc tatctcggtt atatggatga ttacggcgcg
     3601 ttacgcatga cgatgctcaa ctgcagcgga caatgccatt tacaagccgt ggaggcgaag
     3661 aaatgagtgc tggcaaggga gtgttgctcg tcatctgtct gttatttctg ccgttgaagt
     3721 ctgcgcttgc cctgaactgc tactttggtt catccggtgg ctcggtagaa aaatcagaag
     3781 cgattcaacc ttttgctgta ccaggcaatg ccaaactcgg cgataagatt tgggaatctg
     3841 acgatattaa aattcccgtc tattgtgaca acaataccaa cggcaatttt gaaagtgagc
     3901 atgtttatgc ctgggtaaac ccctacccgg gggtgcagga tcgctattat caactcggcg
     3961 taacttacaa tggcgttgat tatgacgcca accagggaaa aagccgcatc gacactaacc
     4021 agtgtattga cagtaagaac attgacattt atacccctga gcagattatc gcgatgggat
     4081 ggcagaacaa aatttgctcg ggtgatcccg ctaatattca catgtcgcgt acctttctcg
     4141 ctcgcatgcg gttatacgtc aaaatacgag aaatgccgcc gcatgattat caaagtacgc
     4201 ttagcgacta tatcgtcgtg caatttgacg gtgccggtag cgttaacgaa gatcctactg
     4261 cccaaaacct gaaatatcat attactggtc tggaaaacat tcgcgtgctg gattgcagcg
     4321 tcaatttttc catttcaccg gaaacacagg tgattgattt tggtaaattt aatttgctgg
     4381 atattcgtcg ccacacaatg tcgaaaacat tcagtattaa gacgacaaaa tcacaaaatg
     4441 accaatgcac cgacggattt aaagtcagtt cctcctttta taccgaagaa acgttggttg
     4501 aagaagataa agcattgttg attggtaatg gtctgaagct acgtttgctg gatgaaaacg
     4561 cctcgcctta taccttcaat aaatatgctg aatacgccga tttcaccagc gacatgttgg
     4621 tctacgaaaa aacctataca gctgaacttt cttccattgc aggtaccccc atcgaggctg
     4681 ggcccttcga tacagtggtg ctttttaaaa ttaactataa ctgatttgcc atgatgccct
     4741 ttgcagccgg aaagggcatc tctttcagaa aattgctgat tttccccttt tccttgattg
     4801 ctgttatttt ttcctggccc ggctttcttt atgctgtatc gatacaccaa agagaaaata
     4861 atgagggagc gaaggatgcc agttttgcag tggggaatgt tatgtgtgct atcacttctc
     4921 ctttctattg gtttcctcgc ggtacatctc ccggcggcgc tattacttgg gccgatgatc
     4981 gccgggatca tcttcagtat gcgcggaatt actctgcaac ttccccgctc cgcttttctc
     5041 gccgcccagg ccattcttgg ctgcatgatt gcgcaaaacc tcactggttc gattctcacc
     5101 acgctggccg ccaactggcc gatcgtgctt gctattttac tggtgacgct gctttccagc
     5161 gccatcgtgg gttggttatt ggtgcgctat agctcactgc ctggaaatac cggtgcctgg
     5221 ggttcttctc caggcggcgc agcggcaatg gtcgctatgg cgcaagatta cggcgcagat
     5281 attcgcctgg tggcgtttat gcaatatctg cgagtgctgt tcgtcgcggg ggccgctgtt
     5341 ctggtcaccc gcatgatgct gggtgataac gctgaagcgg ttaaccagca aatcgtctgg
     5401 ttcccgccag tgagcattaa tctcctgctt acgattttgc tcgcagtcgt tgccggtacg
     5461 gcgggatgca tgctgcgtct tccttccggc acgatgctca tcccgatgct ggcgggcgca
     5521 gtgctccagt ctggtcagct catcaccatc gaactaccgg aatggctgct ggcgatggcg
     5581 tatatggcaa ttggctggcg gattggtctt ggtttcgata aacaaatctt actgcgggca
     5641 ctacgcccac taccgcaaat cctgctgtcg atttttgctc tgctggctat ttgtgcgggt
     5701 atggcgtggg ggctgacccg gtttatgcac attgatttta tgactgccta cctcgccacc
     5761 agtcccggcg ggcttgatac cgtggcggtc atcgccgcag ggagcaatgc cgatatggcg
     5821 cttattatgg cgatgcaaac cctgcgcctg ttcagtattt tgctgacggg gccagccatt
     5881 gcgcggttta tttcaaccta tgcgccgaag cggtcggcct agtgctggca gccagggcac
     5941 cagtaaaacg ggcgagatga cagcgtggtt ttctcaatga tgctgccaca gcgttcgcac
     6001 ggttcgccat cacgatgaaa aaccttaaag cgaaacagcg ccccatgatg cttattctca
     6061 tccacctgcc cccgcgtagc gtaagaaaac cgaggaatct ccagtaacgc gtgtgcgagt
     6121 gcatccagtt gcgccgcatt gagatctttc gctttatgat ttccagtcaa cccaacctgc
     6181 cagaggatct ccacccgcaa ataattgcca agcccagcca gaaacgcctg atcgagcagt
     6241 aatcctgcaa actgacggtt acgaaagcgc ggtgacaata accgttcctt taccacctcc
     6301 ggcgtcagat tcggatccag cacatcggga ccaacgcgtt gtaaaaacgg atgcgtggtc
     6361 agttgttccg gcctcaacat ctcaatatcc gaggcgctat aaagcagaat ggttttgtca
     6421 gccgtttgca gttttacccg caatactcgc gtggtctgcg gctcttcgcc ggtatcaacc
     6481 acgcgccaga cgccgtaaag ctgattatgg ctgtagagcg ttaagtcgtt ggaaaaatga
     6541 gttaacaacg ccttaccacg cgtttccaca tgggtaacgt gttgaccgat aagttgtgat
     6601 tgataagttt ttaactgcgg gaaggcaaac cagacatcag ttagtggttt gcctttgatc
     6661 gccgcctcca ggttatccgc tgcacggcgg atctccgggc cttcaggcat ggtgatatcc
     6721 ttttttcatt ctttattaat cggggttatg ctgcgacaac aatctccttt tcggcaaatg
     6781 tagagcgtag tcggcgggcg aacgccagtg cgtgctcgcc gtcgccatgc aggcagactg
     6841 tttgcgccgt gaccgttgcc cattcgccgg tgatactttt gactctgccg tgttgcacca
     6901 tctctagcgt ttgcgccagc gcctgttctt cgttttcaat caacgcgcct ggctgacttc
     6961 gcggcaccag cgagccgtca gcctgataac cgcgatcggc aaacacttcc tcgcgcgttg
     7021 tcaggccata ttgcttgcct gcacgaatca gctcgcttcc tgccagcccg acgagaacca
     7081 atgctggatc gcaagcgtat accgctctgg cgatggcgtc tgccagttgt gcttctttcg
     7141 ccgcctggtt gtacaacatg ccgtgcggtt tgacatgacg cattacgccg ccttgcgcac
     7201 gggcaatcgt tgccagcgcg ccaatttgat acagcgtctg ggcgtaaacg gtttctggcg
     7261 gcagctgcat ggcgctgcga ccaaaatttt ccctgtcggg aaaactcggg tgagcgccaa
     7321 tcgcaacacc attttttatt gcttcacgca cgcaagcctg catggtttgg gcatcgcctg
     7381 catgaaatcc acaagcaata ttggcagagg aaaccagcgt taatagctct gcgtcgctgg
     7441 cgcagccttc gcccagatcg gcgttcaggt caattttcat tgtgcagccg ccacgctaat
     7501 tgttcaaaat aacgttgctg atcttgccgc gctttcagtg cctcttccag tgaacactgg
     7561 acaaaatgaa tcggctgacc gagcggaatt tgcgccagat ggtacatatc agcctcaatg
     7621 atacaggcaa tacgcgggta accaccggtg gtctgtgcgt cgttcatcaa cacaatcggc
     7681 tgcccgttat gtggcacctg caccacgccc ggtaacaaac cgtgagataa cagttcgcga
     7741 tcggtggtgc gttttaaaat ttgcccctgt aagcgatagc ccatgcggtt actttgcgag
     7801 ctaagctgcc agggcgaacg ccagaatgca tcctgcgagg cgcgatcgaa ctcatgatat
     7861 tccggccccg gcaaggcgcg aatgcggttg ccccacagca gctgtttaac gccctgcgct
     7921 tccatagaat cacgcttcga tttgccaatc gggagtcggt caccatcctt cagtaaacgg
     7981 ccttccagcc cgccaatccc cactttgaga tcggtgctgc atgagcccat taccggcgga
     8041 acatcaatac cacccgcgac cgccagataa ctgcgcatcc cgtgctgcgg gcgtttaagc
     8101 gttaaacgct ggcctgcttt catcggcaat cgccagccgg tccagacggc attatcatcc
     8161 agccgcgctt cgcaaccggc acccgtcaga gcaaaccacc catcagtttc gaactcaaca
     8221 gttaactgac cgagcgtgat ctccagcgcg ggggcattgg cgtcattacc caccagtagg
     8281 ttagcaatgc gtaacgcggg catatccagt gcgccgcagt ggctgatacc cgactggcga
     8341 aaaccgtgac gaccgccatc ctgcacagtg gtatacatgc ccgcacgaat aatcttcagc
     8401 atactccctc cttctgcggt acaaagcgca cgctgtctcc cggacgtaat aagatgggtt
     8461 cgtcacgcgc cggatcaaac aggctgagtg aggtatgacc aatcaactgc cagccacccg
     8521 gcgttgccag cggataaaca ccagtctgcg gcccgccgat cccgacagaa cctgccggaa
     8581 cgagtaagcg cggttcagcg cgccgtggcg tgtgtagttg ttccggcaaa ctcccgagat
     8641 acgggaagcc cggttgaaaa cctaaaaacc agaccacgta ttccacggag gagtgcaatt
     8701 caacaacctg tttttcgctc aacccgcaat gcgccgcgac caccgccaaa tccggtccgc
     8761 ctgcaccacc gtaaaccacc ggaatttcaa taaagcgaga ctccggctcc agcgcctcgc
     8821 tctcctccca ccagcgttgc aaacgctcta tggcatccag cgccagcgac tcaggattac
     8881 gcaaaatcac cgtgatattg ttcatgccgg gaatggcttc aaccacattc ggcatatcca
     8941 ccagacgctg cgccagtcgc cagatccgtt tctggctagc cagcgtcacc ggcggttcca
     9001 gttccagcac taccgccgtt tcacctatca gataacaacg cgctcgttgc actttcgatc
     9061 cctctgatta ttcgttatgc aggattagga atatcaataa aggtcacatc aagatcggta
     9121 ttttcattca gccactcgct caatgcgcga ataccaccac gttcagtggc atggtgacct
     9181 gcagcataaa aatgcaatcc ctgctcgcgg gctgaatgaa tggtctgttc agaaacttcg
     9241 ccagtaataa aagcatccac gccaaaacgc gcggcgctat cgataaaact ttgcccgccg
     9301 cccgtgcacc aggcgacgcg ctggaccacc tcaggtccgg tatcgccaca ccataacggc
     9361 ttacgtccca gacgcgcttc aatccaggaa gccagctcca gtcccggcac aggcatggtc
     9421 agttcgcccc acggcaccaa cggctcaatt tcgcccatga ccgtgatccc cagtaacgcc
     9481 gccagctgtg cgttattgcc cagctcagga tgtgcgtcaa gcggcaaatg ccagccatac
     9541 aggttgatat cattcgccag caacgttttt aaacggttac gcttcattcc gcgaatgacc
     9601 ggagactctc ctttccagaa gtagccatga tgcacaatga ccgcatccgc gcccagacgc
     9661 actgcctcat cgagcaaagc ctggctggcg gtgacaccgg taacaatttt ttgcaccgtc
     9721 tctttgcctt ccacctgcaa accattcggc gcgtaatcac taatcgccgc gctgttcagt
     9781 ttttcgttaa tcagttgttc cagttcggtg tttttcatct tcgcgccttc cgtattctga
     9841 tagctacacc gttaacatag cgttacctcg ccagttcgtc gatagccaat cttttgacat
     9901 attaagaaac aaacccgacg ccaaaagacg caaattgttt atatcatcac gcttcagcta
     9961 atgtatattt atgcaaacag acagcatatt gcataaataa tcagatctgt atcaaagttt
    10021 taactttaag gaaacacaag cacgccgcgc aactattacg tgacgttgcc gtcaattgtg
    10081 gaattattat gaataaacac gcatcacagc cgcgcgctat ttactatgtc gttgcgctgc
    10141 aaatctggga atattttagc ttttacggca tgcgtgccct gctgattctc tatctcacca
    10201 atcaactaaa atacaacgat actcacgcct acgagttatt tagcgcctac tgttcgctgg
    10261 tgtatgtcac gccaatcctc ggtggctttt tggcggataa agttctcggc aatcgcatgg
    10321 cggtgatgct gggggcgttg ttgatggcga tcggtcatgt ggtgctgggt gccagtgaga
    10381 tccatccgtc attcctctat ctgtccctgg cgattatcgt ctgcggctat ggcctgttta
    10441 aatctaacgt aagctgtctg ctcggcgagc tgtatgagcc aaccgatccg cgtcgtgatg
    10501 gcggtttctc gctgatgtat gcggcgggta acgtggggtc tattatcgca cctatcgcct
    10561 gtggttacgc ccaggaagag tacagctggg cgatgggctt tggcctggcg gcggttggca
    10621 tgatcgcggg tctggtcatt ttcttatgtg gcaatcgtca tttcactcat acccgcggcg
    10681 ttaacaaaaa agtactgcgt gcgacaaact ttctcctgcc gaactgggga tggctgctgg
    10741 ttctgctggt ggcaacgcct gcactgatta ccatactgtt ctggaaagag tggtcggtat
    10801 acgccttaat tgtcgcgact attattggcc tgggtgtact ggcaaaaatt tatcgcaaag
    10861 cagaaaacca gaaacagcgg aaggagctgg ggctgattgt gacgctcacc ttcttcagta
    10921 tgttgttctg ggccttcgca caacagggcg gtagctcgat tagcctttat atcgaccgct
    10981 tcgttaaccg cgatatgttt gattataccg ttccgaccgc gatgttccag tcgattaatg
    11041 ccttcgcagt tatgctgtgc ggtgtgttcc tggcgtgggt ggtaaaagag agtgtcgcgg
    11101 gtaatcgtac cgtgcgcatc tgggggaaat ttgctcttgg ccttggcctg atgagcgccg
    11161 gattctgcat tctgacctta agcgcccgct ggtccgcaat gtatggtcac tcttctctgc
    11221 cactgatggt attaggcctg gcggtgatgg gctttgcgga actgtttatc gacccggttg
    11281 ccatgtcgca aattacgcgc attgaaatcc ccggtgtgac cggcgtatta accggcatct
    11341 acatgctgct ttctggcgcg atcgcgaact atctggcggg cgtgattgcc gatcagacat
    11401 cgcaggcttc gtttgatgct tccggggcga tcaactactc catcaatgca tatattgaag
    11461 tatttgatca aattacctgg ggcgcactgg cgtgtgtagg actggtactg atgatttggc
    11521 tgtatcaggc gctgaaattc agaaaccgcg cgctggcgct ggagtcttaa taggccagca
    11581 ccgatgcctg atgcgacgct tgacgcgtct tatcaggcct acgtatttcc tgcaatttat
    11641 tgaatttgca caaatttgta ggctggataa ggcgttcacg ccgcatccgg catctgggct
    11701 cgattgcctg acgcgtctgt tatttcccct tccgcgccgc ctcatacgct gccaacgttt
    11761 gtactctcgc ttctttgtgc tcgactatcg gttgcggata atccagcgtc acacctgctt
    11821 tctgcgccca cttccacggc tcatgcacca ctttccctgg cacatcgcgc agttccggta
    11881 gccactggcg gataaactcg ccctcatgat caaatttctc gccctgggtt gtcgggttga
    11941 aaatacgaaa atacggcgct gcatcggttc cggttgaagc ggcccactgc cagccaccgt
    12001 tattggctgc caaatcacca tcaatcagct gcgacatgaa atatcgctcg ccttcgcgcc
    12061 agtcgatcaa taaatctttc accagaaaac tggctgtaat catccgtagc ctgttatgca
    12121 tccagccagt gctgttaagc tgacgcatag cggcatcaac aatcgggtat cccgttttgc
    12181 cttcctgcca ggcctgtaaa tgtgcgggat tgctctgcca ctgtacacga tccgtccagg
    12241 caataaatgg acgatgttta cacaacgagg ggtgatacgt tatcaggtga cggtaaaact
    12301 cgcgccagat cagctcatta agccagacac taccggcccc accgtccagc gcctgcggct
    12361 gttcagccaa caagcgatgc aagcactggc gaggcgataa cccgcccgtt gccaggctgg
    12421 ccgacaaacg gctggtgcct tccactgccg gaaaatctcg ttgttgctca tattctccgg
    12481 caccgttctg gcaaaactgg cgtaattgcg caatcgccgc tttttcttcc accggaaaat
    12541 gcgcagtatc gaaagactga cgaggataat tcagcgtaat ggatggcgag ggctctatcg
    12601 atccgctact acgaactttt ggcgcagcga cgcactccgg catcccttcc cgcagccgtt
    12661 tcagccaggc attcttaaaa ggcgtaaaga ctttgtacat ctcgtgatta ccggtcatca
    12721 ccgcgccagg cggcaggatc acgctgtcat caaatccttc acacaccacg ttacgcagcg
    12781 ctctttcaac ttccacatcc cgcgcccgct cattcacttc atactgatag ttataaaaca
    12841 ggtgggtaac gctgttttcc gcgcacacct gtttaactat ttcgacactg gcgacaaagt
    12901 catccacttc acggaacaat aaaggaatac ctttttccgc aagcgctatt tgtagcccat
    12961 tcagttgagc attgataagt tcagcctgac gcggcgacat gttatgcgtc gcccactggc
    13021 gtggtgtagc gatatacaac gccagcacgc gtgcagacga attgcggcag gcggcagcca
    13081 gtgcgagatt atcgtgcaga cgtaaatcct ggcgaaacca gaccagatgg gtagtcataa
    13141 aactcctgaa tggcgcaagt taatcacacg cggccaaagt tcgaaataac gctgccctga
    13201 aagccaggcg tcaggataaa gcgccatata gtgtttgata cgcatcagcg gcgcaagaag
    13261 tggttgctcg ccacggcgat aactgtcgat aagcgcagcc agctgctggc gttctgtgga
    13321 atcaatatgc gggcgaaaat aaccctgaac gtgcatcaaa acattggtgt gatcgcgcgg
    13381 gttggcgggg tgagaaagca aaacaactac gcgttggcgg tactggttgt aatagtcatc
    13441 cagattttgc cactggtgaa tatcagccac gaacgggcca agtttgcggt actccggctg
    13501 agaatgcgcc agaaagacga gtttatagcg gctgtgataa tcgagaagcg ccccgcgcgt
    13561 taagccatgt tctttcagtc gatgtaactc atgaagggca aagatacgga ttagggtgct
    13621 gtcatcaaat cgttgtagat tcattactgc tcctgagcgg tttacaggag taagtgtaga
    13681 cgttgagaaa tgatatttgt gaagttaatc ctgttgtagc aggacaaatt tacgaaagcc
    13741 tgctccccgc aaggactgac gtcaggtagt ttctaaccat agctgccctt tctattcaaa
    13801 cgtgaaatca ataagctcat cagggacatc gcaaacaata tgaagacggg tttcttctct
    13861 gccgtgactc ttttcactaa ttgcgtaact gtcatgcttg ggattattta attctttcag
    13921 cggaaatttt tcctcgaagg ctttatttag ccgcccctgg tttcctttta cagcgaataa
    13981 ataatcacct ccctgttttt gtatcttctc tgcaatatct ttctggcaac ccatcgcatc
    14041 ggttttgatg atttttcctt taatatccag catgttaaga agttcaggga tagctgtgat
    14101 ctcattggat ttcttatccg tcttgatctg tccgatgacc agactgtgca ttgttgagaa
    14161 cgcactaatg acatgaatcg ctcccttacg gcgactcttg tcataagaat gccggtgtat
    14221 ttttccatca attgcgatga cgtctttatc atctgaagaa tagtagtcaa gcatccagtt
    14281 aataaagctc tcatgaaatt tcgcaggaca gatacaggat acaactctgg caatggtatc
    14341 gtgaacagga ataccatttt caaaatcacc atattgcttc aaaaaatcgg gatgtgtttc
    14401 cccaaaatcc tctatatctt cccagc
//
LOCUS       DACAPB010000117        14355 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.117, whole genome shotgun sequence.
ACCESSION   DACAPB010000117 DACAPB010000000
VERSION     DACAPB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.117"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..936
                     /locus_tag="IT904_003113"
     CDS             <1..936
                     /locus_tag="IT904_003113"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HAP3192856.1"
                     /translation="LTDSAHAPYQVARFVEKPDRERAEVYLASGEYYWNSGMFMFRAK
                     KYLSELAKYRPDILETCQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAV
                     VVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVEN
                     LVIVSTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRF
                     NVNRITVKPGGAFSMQMHHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHC
                     LENPGCIPLEVLEIQSGSYLGEDDIIRIKDQYGRC"
     gene            960..2342
                     /gene="cpsG"
                     /locus_tag="IT904_003114"
     CDS             960..2342
                     /gene="cpsG"
                     /locus_tag="IT904_003114"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP3192857.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLM
                     GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLNLLKEELL"
     gene            2348..3133
                     /locus_tag="IT904_003115"
     CDS             2348..3133
                     /locus_tag="IT904_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family O-antigen ABC transporter permease
                     subunit Wzm"
                     /protein_id="HAP3192858.1"
                     /translation="MFSAIYRYRGFIIDSVKRDFQSRYQTSFLGAAWLILQPIAMISV
                     YTLIFSELMRARLAGMDGPFAYSIYLCSGVLTWGLFTEMLNSLVNVFLTNANILKKLS
                     FPRICLPIIVTASAFINFLIIFGLFVLFLIVTGNFPGMIFFEIIPVLIVQMLFTLGLG
                     IILGVLNVFVRDVGQFVNILLQFWFWFTPIVYVSKTLPEWVSGLLAYNPMATIIGSYQ
                     NVMLYHQSPNWMALLPVTVVSVILFLFAWRLFKKHAADIVDEI"
     gene            3136..4431
                     /locus_tag="IT904_003116"
     CDS             3136..4431
                     /locus_tag="IT904_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family O-antigen ABC transporter ATP-binding
                     protein Wzt"
                     /protein_id="HAP3192859.1"
                     /translation="MSIKVQHVGKAYKYYPSKWNRVIEKLLPGDKPRHSKKWVLKDIN
                     FSIEPGEAVGIVGVNGAGKSTLLKLLTGTTQPTKGSIEIQGRVAALLELGMGFHPDFT
                     GRQNVYMSGLMMGLGREEIERLMPEIEAFADIGDYIEEPVRIYSSGMQMRLAFAVATA
                     SRPDILIVDEALSVGDSRFQAKCYARIADFKEQGTTLLLVSHSAGDIVKHCDRAIFLK
                     NGDICMDGTARDVTNRYLDELFGKPDKDSATKSATAISSASGESQMSLDEIEDVYHTR
                     PGYRPEEYRWGQGGAKIIDYHIQSAGVDFPPSLTGNQQTDFLMKVVFEYDFDCVVPGI
                     LIKTLDGLFLYGTNSFLASEGRENISVSRGDVRVFKFSLPVDLNSGDYLLSFGISAGN
                     PQTDMTPLDRRYDSIILHVTKSMDFWGVIDLKSSFTSYQ"
     gene            4451..6577
                     /locus_tag="IT904_003117"
     CDS             4451..6577
                     /locus_tag="IT904_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012967601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP3192860.1"
                     /translation="MTKDLNTLVSELPEIYQTIFGHPEWDGDAARDCNQRLDLITEQY
                     DNLSRALGRPLNVLDLGCAQGFFSLSLASKGATIVGIDFQQENINVCRALAEENPDFA
                     AEFRVGRIEEVIAALEEGEFDLAIGLSVFHHIVHLHGIDEVKRLLSRLADVTQAVILE
                     LAVKEEPLYWGVSQPDDPRELIEQCAFYRLIGEFDTHLSPVPRPMYLVSNHRVLINDF
                     NQPFQHWQNQPYAGAGLAHKRSRRYFFGEDYVCKFFYYDMPHGILTAEESQRNKNELH
                     NEMCFLAQPPTGFDTPALLAHGENAQSGWLVMEKLPGSLLSDMLVAGEEIDREKIIGT
                     LLRSLAVLEKQGLWHDDVRPWNVMVDARQHARLIDFGSIVTTPQDCSWPANLVQSFFV
                     FVNELFAENKSWNGFWRSAPVHPFNLPQPWSNWLYAVWQEPVERWNFALLLALFEKKA
                     KLPSAEQQRGATEQWIIAQETVLLELQSRVLNESAGSEALRGQIHTLEQQMAQLQSAQ
                     DAFVEKAQQPVEVSHELTWLGENMEQLAALLQTAQAHAQADVQPELPPETAELLQRLE
                     AANREIHHLSNENQQLRQEIEKIHRSRSWRMTKGYRYLGLQIHLLRQYGFVQRCKHFI
                     KRVLRFVFSFMRKHPQVKHTAVNGLHKLGLYQPAYRLYRRMNPLPHSQYQADAQILSQ
                     TELQVMHPELLPPVVYEIYLKLTKNK"
     gene            6588..9110
                     /gene="wbdA"
                     /locus_tag="IT904_003118"
     CDS             6588..9110
                     /gene="wbdA"
                     /locus_tag="IT904_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105458066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymannosyl
                     GlcNAc-diphospho-ditrans,octacis-undecaprenol
                     2,3-alpha-mannosylpolymerase WbdA"
                     /protein_id="HAP3192861.1"
                     /translation="MHILIDVQGYQSESKVRGIGRSTLAMSRAIIENAGEHRVSILIN
                     GMYPIDNINDVKMAYRDLLTDEDMFIFSAVAPTAYRHIENHGRSKAAQAARDIAIANI
                     APDIVYVISFFEGHSDSYTVSIPADNVPWKTVCVCHDLIPLLNKERYLGDPNFREFYM
                     NKLAEFERADAIFAISQSAAQEVIEYTDIASDRVLNISSAVGEEFAVIDYSAERIQSL
                     KDKYSLPDEFILSLAMIEPRKNIEALIHAYSLLPAELQQRYPMVLAYKVQPEQLERIL
                     RLAESYGLSRSQLIFTGFLTDDDLIALYNLCKLFVFPSLHEGFGLPPLEAMRCGAATL
                     GSNITSLPEVIGWEDAMFNPHDVQDIRRVMEKALTDEAFYRELKAHALAQSAKFSWAN
                     TAHLAIEGFTRLLQSSQETDAGQAESVTASRLQMMQKIDALSEVDRLGLAWAVARNSF
                     KRHTRKLLVDISVLAQHDAKTGIQRVSRSILSELLKSGVPGYEVSAVYYTPGECYRYA
                     NQYLSSHFPGEFGADEPVLFSKDDVLIATDLTAHLFPELVTQIDSMRAAGAFACFVVH
                     DILPLRRPEWSIEGIQRDFPIWLSCLAEHADRLICVSASVAEDVKAWIAENRHWVKPN
                     PLQTVSNFHLGADLDASVPSTGMPDNAQALLAAMAAAPSFIMVGTMEPRKGHAQTLAA
                     FEELWREGKDYNLFIVGKQGWNVDSLCEKLRHHPQLNKKLFWLQNISDEFLAELYARS
                     RALIFASQGEGFGLPLIEAAQKKLPVIIRDIPVFKEIAQEHAWYFSGEAPSDIAKAVE
                     EWLALYEQNAHPRSENINWLTWKQSAEFLLKNLPIIAPAAKQ"
     gene            9157..10302
                     /locus_tag="IT904_003119"
     CDS             9157..10302
                     /locus_tag="IT904_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP3192862.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIDQIPQVENKSDSKASNHGRLSAFLRRQPLLIEAYRLLHPRRQAWALRDYKDYIYHG
                     PNFYLPHRLERAVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSR
                     NEIIRLFNYPADRIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQGYALYIGTMEPRK
                     NIRGLLQAYQLLPMETRMRYPLILSGYRGWEDDVLWQLVERGTREGWIRYLGYVPDED
                     LPYLYAAARTFVYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDV
                     DAISAHILQSLQDDSWREIATARGLAQAKQFSWENCTTQTINAYKLL"
     gene            10312..11427
                     /locus_tag="IT904_003120"
     CDS             10312..11427
                     /locus_tag="IT904_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP3192863.1"
                     /translation="MRVLHVYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KETGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKQIKDDYDIINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLASVDCIVASSPNYVASSQTLKKY
                     QDKTVVIPFGLEQHDVQHDPQRVAHWRETVGDNFFLFVGAFRYYKGLHILLDAAERSR
                     LPVVIVGGGPLEAEVRREAQQRGLSNVVFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(11466..12074)
                     /locus_tag="IT904_003121"
     CDS             complement(11466..12074)
                     /locus_tag="IT904_003121"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAP3192864.1"
                     /translation="MLTEQQRHELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(12068..12844)
                     /gene="hisF"
                     /locus_tag="IT904_003122"
     CDS             complement(12068..12844)
                     /gene="hisF"
                     /locus_tag="IT904_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAP3192865.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINISELKAYLATQGVEIRIC"
     gene            complement(12826..13563)
                     /gene="hisA"
                     /locus_tag="IT904_003123"
     CDS             complement(12826..13563)
                     /gene="hisA"
                     /locus_tag="IT904_003123"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAP3192866.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(13563..14153)
                     /gene="hisH"
                     /locus_tag="IT904_003124"
     CDS             complement(13563..14153)
                     /gene="hisH"
                     /locus_tag="IT904_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAP3192867.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGANLLKNFLEM"
     gene            complement(14153..>14355)
                     /locus_tag="IT904_003125"
     CDS             complement(14153..>14355)
                     /locus_tag="IT904_003125"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HAP3192868.1"
                     /translation="VGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAF
                     GRTLRQAIRVEGDTLPSSKGVL"
BASE COUNT         3132 a         3867 c         4013 g         3343 t
ORIGIN      
        1 ctcaccgaca gcgcccacgc cccgtaccag gtggcccgct tcgtggagaa gccggaccgc
       61 gagcgcgccg aggtctacct cgcctccggg gagtactact ggaacagcgg catgtttatg
      121 ttccgtgcca aaaaatacct ctctgagctg gccaaatacc gtccggatat cctggaaacc
      181 tgccaggctg cggtgaatgc cgccgataat ggcagcgatt tcatcaatat cccacatgat
      241 attttctgcg agtgcccgga cgagtccgtg gactacgccg ttatggagaa aaccgctgat
      301 gcggtggtgg tcggtctcga tgctgactgg agcgacgttg gctcctggtc cgcactatgg
      361 gaggtcagcc cgaaagacgg gcagggtaat gtcctcagcg gtgacgcgtg ggtgcacaac
      421 agcgaaaact gctacatcaa cagcgacgag aagctggtgg cggccatcgg tgtggagaac
      481 ctggtgattg tcagcaccaa ggacgccgtg ctggtgatga accgcgagcg ctcccaggac
      541 gtgaagaagg cggtcgagtt cctcaagcag aaccagcgca gcgagtacaa gcgccaccgt
      601 gagatttacc gtccctgggg ccgctgcgac gtggtggtcc agaccccgcg cttcaacgtc
      661 aaccgcatca cagtgaaacc tggcggtgcc ttctcgatgc agatgcacca ccatcgcgct
      721 gagcactggg ttattctcgc cggcaccggt caggtgacgg tcaacggcaa gcagttcctg
      781 ttgtccgaga accagtccac ctttattccg attggcgccg agcactgcct ggaaaaccct
      841 ggctgtattc cgctggaagt gctggagatc cagtcggggt cgtacctcgg cgaggacgac
      901 attattcgta ttaaagacca gtatggtcgt tgctaattat tttcgggaca agatgcagaa
      961 tgacacagtt aacatgcttt aaggcttatg acatccgtgg tgaactgggt gaggaactga
     1021 acgaggacat cgcctaccgt atcgggcgcg cctacggcga atttctgaaa cccgggaaga
     1081 tagtggtggg gggggatgtg cgcctcacaa gcgagtcgct gaagctggcg ctggcccgcg
     1141 ggttaatgga cgccggtacc gatgtgctgg acatcggact gagcggcacg gaagagattt
     1201 acttcgccac cttccacctc ggggtggacg gcggcatcga ggtgacggcg agccacaacc
     1261 cgatgaacta caacggcatg aagctggtgc gcgagaacgc gaagcccatc agcggcgaca
     1321 ccggcctgcg ggatatccag cgcctggcgg aggagaacca gttcccgccg gtggacccgg
     1381 cacgtcgcgg gaccctgcgc cagatttcgg tgctgaagga gtacgtcgac cacctgatgg
     1441 gctacgtgga cctggcgaac ttcacccgtc cgctgaagct ggtggtgaac tccggcaacg
     1501 gggcggcggg gcacgtgatt gatgaggtgg agaaacgctt cgcggcggcc ggggtgccgg
     1561 taacctttat caaggtgcat caccagccgg atggccattt ccctaacggt atccctaatc
     1621 cgctgctgcc ggagtgccgc caggataccg ccgacgcggt gcgcgagcat caggccgaca
     1681 tggggattgc ctttgacggc gacttcgatc gctgcttcct gttcgatgac gaggcgtcgt
     1741 ttatcgaggg gtactacatt gtcggcctgc tggcggaggc gttcctgcag aagcagccgg
     1801 gggcgaaaat cattcacgac ccgcgcctga cgtggaacac ggtggacatc gtgacccgca
     1861 acggcggcca gccggtgatg tcgaagacgg ggcatgcgtt catcaaggag cggatgcgtc
     1921 aggaagacgc tatctacggc ggggagatga gtgcgcacca ttacttccgc gatttcgcct
     1981 actgcgacag cgggatgatc ccgtggctgc tggtggcgga gctgctgtgc ctgaagaaca
     2041 gctcgctgaa atcgctggtg gcggaccgcc aggcggcgtt cccggcgtcg ggggagatca
     2101 accgcaagct gggtaatgcg gcagaggcga tagcgcgcat ccgggcgcag tatgagccgg
     2161 cggctgcaca catcgacaca acggacggga tcagtattga ataccctgaa tggcgcttta
     2221 acctgcgcac gtctaacacc gagccggtgg tgcgtctgaa cgttgagtcc agagctgatg
     2281 tggcgcttat gaatgaaaaa acgaccgagc tgctcaacct gttaaaagag gaattgcttt
     2341 gagtccgatg ttttcagcga tctatcgcta ccgtggcttt attattgaca gcgttaaacg
     2401 ggactttcag tcccgttacc aaaccagctt cttaggcgcg gcatggctga ttttacagcc
     2461 gatcgccatg atttccgtct atacattaat tttttctgaa ttaatgcgtg cccgcctggc
     2521 tggcatggat ggtccttttg catacagcat ctacttgtgt tctggggtgt taacttgggg
     2581 gctgttcacg gaaatgctca acagtctggt caacgttttt ctgaccaacg ccaatattct
     2641 taagaagctc agctttccgc ggatctgttt accgattatc gtcactgctt cggcatttat
     2701 taacttcctg atcatttttg gtctgttcgt gctgtttctg atcgtcacgg gcaatttccc
     2761 tggcatgatt ttctttgaaa tcattccggt gctgatcgtc cagatgctgt tcaccctcgg
     2821 cctcgggatc attctcgggg tactgaacgt ttttgtccgc gatgtcgggc agttcgttaa
     2881 tatcctgctt cagttttggt tctggtttac gccaattgtc tacgtatcca aaacgctgcc
     2941 ggaatgggtt tctggtctgt tggcgtataa cccgatggcg accattatcg gttcctacca
     3001 gaacgtgatg ctctatcacc agagccctaa ctggatggcg ctgcttccgg tcacagtggt
     3061 gtcggtcatt ctgtttttat ttgcctggcg tttatttaaa aaacatgccg ctgatattgt
     3121 ggacgagatt taatcatgag tatcaaagtt cagcacgtcg gcaaggcgta taaatattat
     3181 ccatctaaat ggaaccgggt cattgagaaa ctactgccgg gcgataagcc gcggcacagc
     3241 aagaaatggg tattgaaaga tatcaatttc agcattgaac ctggggaagc ggtcggcatt
     3301 gttggggtga acggcgcagg taaaagtacg ttactgaagc tgctgactgg caccactcag
     3361 cccaccaaag gtagcattga aatccagggg cgcgtcgccg cgctgctgga gctgggcatg
     3421 ggcttccatc ctgactttac cggccggcag aacgtgtata tgtccgggct gatgatgggc
     3481 ctgggccggg aagagatcga gcgcttaatg ccggagatcg aagccttcgc cgatatcggc
     3541 gactacattg aagagcccgt acgcatctat tccagcggga tgcaaatgcg cctggcgttc
     3601 gctgtcgcca cggcctcccg gccggatatt ctgatcgtcg atgaagcgct ctccgtgggt
     3661 gactcccgtt ttcaggcgaa atgctatgct cgcatcgcgg acttcaaaga gcaggggacc
     3721 acgctgctgc tggtttccca cagcgccggg gatatcgtca agcactgcga ccgcgccatt
     3781 ttcctcaaaa atggtgacat ctgcatggac ggcaccgccc gcgacgtaac caaccgttac
     3841 ctggatgagc tgtttggcaa accggataaa gacagcgcga caaaaagcgc aacggctatc
     3901 tcgtcagcca gtggcgaaag ccagatgtct ctcgatgaga ttgaagatgt gtaccacacg
     3961 cgcccaggct accgtccgga agaatatcgc tgggggcagg gtggcgcgaa aatcatcgat
     4021 tatcatatcc agagcgccgg ggttgatttt cctccctcac tgacgggcaa tcagcagacc
     4081 gattttctga tgaaggtcgt gtttgaatac gattttgatt gcgtggtgcc aggcatcctg
     4141 attaagaccc tcgatggctt attcctctac ggaaccaact ctttcctcgc ctctgaaggg
     4201 cgggaaaata tttcggtttc ccgcggcgat gttcgggtct ttaaatttag ccttccggtt
     4261 gatttaaata gtggcgatta cctgctgtca tttggtatct ctgccggcaa cccgcagacg
     4321 gatatgacgc cgctggacag acgttacgac tccattattt tacatgtgac caagagcatg
     4381 gatttctggg gtgtcatcga tcttaagtcg tcctttacta gctaccaatg acgttgagaa
     4441 aaacgattcc atgactaaag acttaaacac gctggttagc gaattacctg agatttatca
     4501 aactattttt ggccatcccg aatgggatgg cgacgccgct cgtgattgta atcaacgcct
     4561 cgatttgatt accgaacagt acgataactt atcccgtgcg ctggggcgcc cgcttaacgt
     4621 tctcgatctg ggctgcgcgc agggcttttt tagcctgagt ctggcaagca aaggcgcgac
     4681 tatcgttggt atcgatttcc agcaggaaaa tattaacgtg tgccgcgcgc tggccgaaga
     4741 gaaccccgat tttgccgccg agttccgggt aggcagaatt gaagaggtga tcgccgcgct
     4801 ggaagagggc gagtttgatc tggccattgg cttaagcgtg tttcaccata ttgttcacct
     4861 tcatggtatc gatgaagtta aacgtctgct ctcgcgcctg gccgatgtca cccaggcggt
     4921 gatccttgag ctggcggtga aagaagaacc gctttactgg ggcgtttctc agccggacga
     4981 cccgcgcgaa ctgattgagc agtgcgcgtt ttatcgcctg atcggtgagt ttgacaccca
     5041 tttatccccc gtaccgcgcc cgatgtacct ggtcagcaac caccgggtgc tgatcaacga
     5101 ttttaatcag ccgttccagc actggcagaa tcagccctat gccggggccg gcctggcgca
     5161 taagcgcagc cgtcgctact tctttggcga agattatgta tgcaagtttt tctattacga
     5221 catgccccac ggcatcctga cggcggaaga gagccagcgt aataaaaacg aactgcataa
     5281 tgaaatgtgt tttctggcac aaccccctac gggctttgac acgccggcat tactagccca
     5341 cggcgaaaat gcccagtctg gttggctggt gatggaaaaa ctcccgggta gcttgctgag
     5401 cgatatgctg gtggccgggg aagagatcga tcgtgaaaaa atcattggca ccttgctccg
     5461 ttccttagcg gtgctggaga agcaagggtt atggcatgac gatgtgcgcc cgtggaacgt
     5521 gatggtcgat gcacggcagc atgcccgctt aatcgacttt ggttctatcg tcaccacgcc
     5581 acaggactgc agctggccgg caaaccttgt ccagtcgttc ttcgtgtttg tgaatgagct
     5641 gtttgccgaa aacaaaagct ggaacggttt ctggcgctcg gcgcctgttc atccgtttaa
     5701 tctgcctcag ccgtggtcta actggctgta tgcggtctgg caggagccgg ttgaacgctg
     5761 gaactttgcc ctgctgttgg cgctgttcga gaagaaagcg aagctgccgt cagctgaaca
     5821 gcagcgcggg gcgacggaac agtggatcat cgctcaggag acggtgctgc tggagctcca
     5881 gtcccgggtg ctaaatgaaa gcgccggctc agaagcgttg cgcggccaaa tccataccct
     5941 ggagcagcag atggctcagc tgcagtccgc tcaggatgcg ttcgtcgaga aagctcaaca
     6001 gccagtggaa gtcagccatg aactcacctg gcttggtgag aatatggagc aactggcggc
     6061 gctgttgcag acggcgcaag cgcacgccca ggctgacgtt caacccgagt tgccccctga
     6121 aaccgctgag ctactgcagc gactggaagc cgctaaccgg gagatccacc atttaagcaa
     6181 cgaaaaccaa cagcttcgcc aggaaattga aaaaatccat cgcagccgtt cctggagaat
     6241 gaccaagggt tatcgttatc tgggcctgca gatccatttg ctgaggcagt atggtttcgt
     6301 gcagcggtgt aaacatttta ttaagcgcgt cctgcggttt gttttctcat ttatgagaaa
     6361 acatccgcag gtaaaacata ccgcggtaaa tggtttacat aagctggggc tttatcagcc
     6421 ggcctatcgt ttgtatcgtc gtatgaatcc actgccgcac agccagtatc aggctgatgc
     6481 gcagatttta tcgcagactg aactgcaagt gatgcatccg gaacttttac ctcccgtggt
     6541 ttatgaaatt tatctcaaac taacgaaaaa taaataagga gattaacttg catattttga
     6601 ttgatgtcca gggttaccag tcggaaagta aagtccgcgg tattggtcgc agtaccctgg
     6661 ccatgagccg cgccattatt gaaaatgccg gtgagcatcg cgtcagtatt ttgatcaatg
     6721 gtatgtatcc cattgataat attaatgatg ttaaaatggc ctatcgggac ctgctgacgg
     6781 atgaagatat gttcatcttc tcagccgtcg cgccaacagc ctatcgtcat attgaaaacc
     6841 atggccgcag taaagcggcc caggcggccc gcgatatcgc tatcgccaat atcgcgcctg
     6901 atatcgtcta tgttattagc ttcttcgagg ggcatagcga tagttatacc gtgtctattc
     6961 cggcggataa tgtgccgtgg aaaacggtgt gcgtatgcca cgatctgatc ccactgttaa
     7021 ataaagagcg ttatctcggc gatccgaatt tccgtgagtt ttatatgaat aagctggcgg
     7081 aatttgagcg ggcagacgcg atcttcgcca tttcgcagtc ggcagctcag gaagtgatcg
     7141 aatataccga catcgccagc gatcgggtac tgaacatctc ctcggcggtg ggcgaagagt
     7201 ttgcggtgat cgattattcc gctgagcgta ttcagtcttt aaaagacaaa tacagcctgc
     7261 cggatgagtt tatcttgtcg ctggcgatga tcgagccgcg gaaaaatatt gaagcgctta
     7321 ttcacgccta cagcttgctg cctgccgagc tgcagcagcg ctatccgatg gtgctggcgt
     7381 ataaagtgca gccagaacaa ctggagcgga tcctgcgtct ggcggaaagc tatggtttgt
     7441 cacgcagcca gcttatcttt actgggttcc tgaccgacga cgatctgatt gccctgtaca
     7501 acctgtgcaa actgtttgtg ttcccgtcgc tgcatgaagg tttcggcctg ccgccgctgg
     7561 aagcgatgcg ctgcggggcg gcgaccttag gttcaaacat taccagcctg ccggaagtca
     7621 ttggctggga agatgccatg ttcaatccgc atgatgtgca ggacattcgc cgggtcatgg
     7681 agaaggcgct gaccgatgag gcgttttatc gtgagctgaa ggcgcatgct ctcgcgcagt
     7741 cggccaaatt ctcgtgggcc aataccgccc atctggcgat cgagggtttc actcgtctgc
     7801 tgcagtcgtc ccaagagacg gatgccgggc aggcggaaag cgtgaccgcc tcccgccttc
     7861 agatgatgca gaaaatcgat gcgctgagcg aagtcgaccg tctaggtctg gcgtgggcgg
     7921 ttgcgcgcaa tagctttaag cgccataccc gcaagctgct ggtggacatt tcggttctgg
     7981 cgcagcatga cgcgaagacc gggatccagc gcgtctcgcg cagtatcctc agcgaattac
     8041 tgaaatctgg cgtgccgggt tacgaggttt ccgccgtcta ctacacccct ggcgagtgtt
     8101 atcgctacgc caaccaatat ctgtccagtc atttcccggg cgaatttggt gctgacgaac
     8161 cggtgctgtt cagcaaagac gatgtgctta ttgccaccga tctgaccgcg catctcttcc
     8221 cggagctggt gacgcaaatt gacagcatgc gcgccgccgg ggcgttcgcc tgcttcgtgg
     8281 tgcatgatat tctgccatta cgccgtccgg agtggagtat cgaaggcatt cagcgtgatt
     8341 tcccgatctg gctgtcctgc ctcgcagagc acgccgaccg gctgatctgc gtctctgcca
     8401 gcgtcgccga ggatgtgaaa gcgtggattg cggaaaatcg ccattgggtg aaaccgaacc
     8461 cgctgcagac cgtcagcaac ttccatctgg gagccgacct cgatgccagc gtaccgtcca
     8521 ctggcatgcc ggataatgca caggcgctgt tagcagcgat ggctgcagct ccgtcattta
     8581 tcatggtggg taccatggag ccgcgcaaag gccacgccca gacgctggcc gccttcgagg
     8641 aactgtggcg cgagggcaaa gactacaact tgtttatcgt cggcaaacag ggctggaacg
     8701 ttgacagctt gtgcgaaaaa ttacgccatc atccccagct gaacaaaaag ctcttctggc
     8761 tgcagaatat cagcgacgag tttttggccg agctatacgc tcgttcacgc gcgctgatct
     8821 ttgcctcgca gggagaaggc tttggcctgc cgttgattga agcggcgcag aaaaagctgc
     8881 cggtgattat tcgcgacatt ccggtgttta aagagattgc tcaggaacat gcctggtatt
     8941 tctccggtga agcgccgtcc gatatcgcga aggcggtaga agagtggtta gccctgtacg
     9001 agcaaaacgc gcatcctcgt tccgaaaata tcaactggtt aacctggaaa cagagcgcgg
     9061 aatttctcct gaaaaacctg ccgattatcg cgccagccgc gaagcaataa tcgctatcaa
     9121 cagggctact gcggtggccc ttgtgatgag ttcgttatga aaattatttt tgctactgag
     9181 ccaattaaat acccattaac gggcatcggt cggtattccc tggagctggt taagcggctg
     9241 gcggttgccc gtgaaatcga agagctgaag ctgtttcacg gcgcgtcgtt tatcgaccag
     9301 atcccccagg tggagaataa aagcgatagc aaagccagca atcatggtcg tctgtcggcg
     9361 tttctgcgcc gccagccgct gctgattgag gcgtatcgcc tgctgcatcc gcggcgccag
     9421 gcgtgggcat tgcgcgatta caaagactac atttaccatg gtcccaattt ctatctgccg
     9481 catcgcctgg aacgcgccgt gaccacgttt catgacatct ccatttttac ctgcccggaa
     9541 tatcatccaa aagatcgggt tcgctatatg gagaagtccc tgcacgagag cctggattcg
     9601 gcgaagctga tcctgaccgt ctccgacttc tcgcgcaatg aaatcatccg cctgttcaac
     9661 tatccggcgg atcggatcgt caccaccaag ctggcctgca gcagcgacta tattccgcgc
     9721 agcccggcgg agtgcctgcc ggtcctgcag aaatatcagc tggcgtggca ggggtatgcg
     9781 ttatatatcg gcaccatgga gccgcgtaaa aatatccgcg gtctgctgca ggcctatcag
     9841 ctgctgccga tggagacccg catgcgctac ccgctgatcc tcagcggtta tcgcggatgg
     9901 gaagacgatg tgctgtggca gttagtcgag cgcggtacgc gtgaagggtg gatccgttac
     9961 ctgggctatg tcccggatga agacctgcct tatctgtacg cggcggccag aacctttgtt
    10021 tatccctcct tctatgaggg attcggttta cctattcttg aagcgatgtc ttgcggtgtg
    10081 ccggtagtat gttccaatgt cacttctttg cctgaggtgg ttggcgatgc cggcctcgtt
    10141 gccgatccta atgatgtaga cgcgattagc gcacatattt tgcagagcct gcaggatgat
    10201 agctggcggg aaatcgccac cgcgcgcggt cttgcccagg cgaaacagtt ttcgtgggag
    10261 aactgtacga cccagaccat taacgcctat aaattactct aagggtgtca gttgagagtt
    10321 ctacacgtct ataagaccta ctatcccgat acctacggcg gtattgagca ggtcatttat
    10381 cagctaagtc agggctgcgc ccgccgggga atcgcagccg atgtttttac ttttagcccg
    10441 gacaaagaga caggtcctgt cgcctacgaa gaccatcggg tcatttataa taagcagctt
    10501 tttgaaattg cctccacgcc gttttcgttg aaagcgttaa agcgttttaa gcagattaaa
    10561 gatgattacg acatcatcaa ctaccatttt ccgtttcctt tcatggatat gttgcatctc
    10621 tcggcgcggc ctgacgcaag aacggtggtg acctatcact cggatattgt gaaacaaaaa
    10681 cggttaatga agttgtacca gccgctgcag gagcgattcc tcgccagcgt agactgcatc
    10741 gtcgcctcgt cgcccaacta cgtggcctcc agccagaccc tgaaaaaata tcaggataaa
    10801 accgtggtga tcccgtttgg tctggagcag catgacgttc agcacgatcc gcagcgggtg
    10861 gcgcactggc gggaaaccgt cggcgataac ttcttcctct tcgtcggcgc tttccgctac
    10921 tacaaagggc tgcacattct gctggatgcc gccgagcgta gccggctgcc ggtggtgatc
    10981 gtcgggggcg ggccgctgga ggcggaagtg cggcgtgagg cgcagcaacg cgggctgagc
    11041 aatgtggtgt ttaccggcat gctcaacgac gaagataagt acattctctt ccagctctgc
    11101 cggggcgtgg tattcccctc gcatctgcgc tctgaggcgt ttggcattac gttattggaa
    11161 ggcgcacgct ttgcaaggcc gctgatctct tgcgagatcg gtacaggtac ctctttcatt
    11221 aaccaggaca aagtgagtgg ttgcgtgatt ccgccgaatg atagccaggc gctggtggag
    11281 gcgatgaatg agctctggaa taacgaggaa acctccaacc gctatggcga aaactcgcgt
    11341 cgtcgttttg aagagatgtt tactgccgac catatgattg acgcctatgt caatctctac
    11401 actacattgc tggaaagcaa atcctgagag ctgctacccc cgccgttgct gcgggggcca
    11461 acgcgttaat gccgattctt cagattatca atcacttctc cgagatccag cccctgatcc
    11521 tgcaacagca ccagcaggtg atacatcaaa tcagatgcct cgttggtcag ctcaaagcgg
    11581 tcatttaccg tcgcggccag tgcggtttcc accccttctt cgcccacttt ctgcgcaatg
    11641 cgtttggtgc cgctggcata cagtttggct gtgtaagagg tttccgggtc ggcagatttg
    11701 cgttcggcga gcagttgttc cagttgatac aggaacagcc actggtgagc ggtgtcgccg
    11761 aagcagctgc tggtgccttt gtggcaggtt ggcccgatgg gattcgccag caccagtaac
    11821 gtgtcgttgt cgcagtccgg ggcaatactc accacattga gaaaattgcc agacgtttcg
    11881 cctttggtcc acagccgttg tttagttcgc gagaagaagg tgactttgcc gctttcgata
    11941 gttttgtcca gagcttccgg gttcatatag cccagcatta acacttcgcc agataccgcg
    12001 tgttgtacaa tcaccggcat cagtccgtcg gttttttccc agtccagttc gtgacgttgt
    12061 tgttctgtta acatatcctg atctccacgc cctgtgttgc caggtacgct tttaattcac
    12121 taatattgat tatttgtttg tggaatacag aagctgccag cgcaccgtca acgtcggcat
    12181 cgcggaaggc ttcgaggaag tgttccatgg tgcccgcgcc accggaggca atcagcggga
    12241 cgtggcaaac ttcacgcact tttttcagtt gttctaggtc gtaaccgtta cgcacgccgt
    12301 cctgattcat catgttgagg acgatttctc cggcaccgcg tttttgcact tcctctaccc
    12361 agtcgagcgt ttcccactga gtgacgcggg tgcggctttc atcgccggta tattgattca
    12421 catgatattt accggtttca gcgtcgtacc aggtatcaat accgaccaca atacactgca
    12481 cgccaaagcg atccgccagg cgagtaatta acgtcgggtc cgccagcgca ggagagttga
    12541 tggaaatttt atccgcgcca aaggaaagaa ttttcgcggc atcttccaga gacttaatcc
    12601 cacccgccac acagaacgga atgtcgatca cttccgccac gcgagatacc cagcttttat
    12661 ctaccacacg gccatcgctg gaagcggtga tatcgtagaa caccagttcg tctgcacctt
    12721 cttcagcgta gcgttttgcc agcggtacaa tatcgccaat gatttcatgg ttgcgaaact
    12781 gtacgccttt caccacctga ccatcacgaa cgtcgagaca tgggattatg cgttttgcca
    12841 gcatgcgatg gcctccttca cggtgaattt accttccagt aatgcacgac caactattac
    12901 gccgcgcacg ccagtaccac gcagggccgc gacatcatca atgtcgccaa taccgccgga
    12961 agactgaaat gccacctgcg gatatctggc gcacacttct tcatataaag agacgttaga
    13021 acctgccagc gtgccgtcgc gcgagatatc ggtacacagc acatgtttca ggccgacggg
    13081 cagataggtt tccaccagtt gttccagtga aacgcccgag ttctcttgcc agccgctgac
    13141 tgccacctgc ttgttgcctt gctcgtcaat acggacatcc agcgccagca ctaaggcatc
    13201 ggcaccgaag cgttcaaacc agcctttcac catttctggt gatttcaccg cggtggagcc
    13261 aaccaccacg cgcgcaacgc ccgcttccag taacgccgcc acgtcttctt cgctacgcac
    13321 gccgccgcca acctgcaccg gaacgttaac gcccgcgacc agggttttaa tcagcgggat
    13381 ttgacgttta gccggatctt ttgccccggt cagatccacc agatgcaaca cttcggcacc
    13441 ctgcgcggcg taatcctgta agcgtggcag cgggtcgtta ccgtaatcgc gctgtttgcc
    13501 gtaatcgccc tgatggagac gcaccacggt gccgtcgatt aaatctaatg ccggaataat
    13561 catcacatct ccaggaagtt tttcagcaaa ttagcgccag cggcaccaga acgctccggg
    13621 tggaactgca cgccgtagaa gttatctttt tgtaccgccg cggtgaacgg ttcgccgtaa
    13681 ttacactggg cgatggtcca cggattgacc ggcattgcgt agctgtgaac aaagtaaaag
    13741 tacgcgccgt cttcaatccc ctgaaacagg cggttgcctg cctgcggata aacgcggttc
    13801 cagcccatat gtggcagtgg cagaccaaag tcggtcattt tcggcacgtc ttcgtcgatg
    13861 atgcccagca agtcgacgcc attgctctct tcgctgcgcc gccccagaag ttgcatccct
    13921 aagcagatgc ccagcaccgg ttgggtacag gctttgatga gatcaaacag ctcgcgctca
    13981 cgcacctggt ccatcgccgc ttgcgcggtg ccaacgccgg gtaaaaacag tttatcggcc
    14041 agcaacacga cgtccgggtc acggctgact ttgggttcat aaccgtggcg cgcaatggca
    14101 gacttcaccg agttcaggtt ggcgcagcca gtatcaagga tcaccacgtt cattacagca
    14161 ctcctttcga cgagggcagg gtgtcgcctt ccacgcggat ggcctggcgc agggtgcggc
    14221 caaacgcttt gaacaggctc tctacacggt ggtgatcgtt tttacctttg gttttcaggt
    14281 gtagcgtcac gcccatggtg tatgaaagcg aacggaagaa gtgctcgatc atttcagtgc
    14341 tgagatcgcc cacac
//
LOCUS       DACAPB010000118        14217 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.118, whole genome shotgun sequence.
ACCESSION   DACAPB010000118 DACAPB010000000
VERSION     DACAPB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.118"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(104..2767)
                     /gene="aceE"
                     /locus_tag="IT904_003126"
     CDS             complement(104..2767)
                     /gene="aceE"
                     /locus_tag="IT904_003126"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAP3192869.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(2928..3692)
                     /gene="pdhR"
                     /locus_tag="IT904_003127"
     CDS             complement(2928..3692)
                     /gene="pdhR"
                     /locus_tag="IT904_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAP3192870.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            4233..5606
                     /gene="aroP"
                     /locus_tag="IT904_003128"
     CDS             4233..5606
                     /gene="aroP"
                     /locus_tag="IT904_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAP3192871.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(5649..6503)
                     /gene="ampE"
                     /locus_tag="IT904_003129"
     CDS             complement(5649..6503)
                     /gene="ampE"
                     /locus_tag="IT904_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAP3192872.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(6500..7051)
                     /gene="ampD"
                     /locus_tag="IT904_003130"
     CDS             complement(6500..7051)
                     /gene="ampD"
                     /locus_tag="IT904_003130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAP3192873.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            7139..8032
                     /gene="nadC"
                     /locus_tag="IT904_003131"
     CDS             7139..8032
                     /gene="nadC"
                     /locus_tag="IT904_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAP3192874.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            8235..8675
                     /gene="ppdD"
                     /locus_tag="IT904_003132"
     CDS             8235..8675
                     /gene="ppdD"
                     /locus_tag="IT904_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAP3192875.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            8685..10070
                     /gene="gspE"
                     /locus_tag="IT904_003133"
     CDS             8685..10070
                     /gene="gspE"
                     /locus_tag="IT904_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAP3192876.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCIHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            10060..11262
                     /gene="hofC"
                     /locus_tag="IT904_003134"
     CDS             10060..11262
                     /gene="hofC"
                     /locus_tag="IT904_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAP3192877.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELAHQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWMQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(11297..12340)
                     /gene="guaC"
                     /locus_tag="IT904_003135"
     CDS             complement(11297..12340)
                     /gene="guaC"
                     /locus_tag="IT904_003135"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP3192878.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            12565..13185
                     /gene="coaE"
                     /locus_tag="IT904_003136"
     CDS             12565..13185
                     /gene="coaE"
                     /locus_tag="IT904_003136"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP3192879.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            13185..13928
                     /gene="zapD"
                     /locus_tag="IT904_003137"
     CDS             13185..13928
                     /gene="zapD"
                     /locus_tag="IT904_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAP3192880.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            13938..14135
                     /gene="yacG"
                     /locus_tag="IT904_003138"
     CDS             13938..14135
                     /gene="yacG"
                     /locus_tag="IT904_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAP3192881.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         3430 a         3733 c         3630 g         3424 t
ORIGIN      
        1 gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg
       61 atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt
      121 taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga
      181 tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa gcatcaactt
      241 cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta
      301 cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt
      361 cggtagatgc cactgccgga gcgtcgttca tcacctgagc gatatacgga acgcgcggag
      421 tttccagcgg gtgcagcatg ttccagcgtt cacaatcctg accatcacgc gccagctcgg
      481 tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctcag
      541 ctgcttcacg gacgtgacgc aggatagaac cggagccgag cagctgaact ttacctttgc
      601 taccttcaat agtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg
      661 gcattgccgg catgtggtag ttttcgttca gcgtagtgat gtagtagtaa acgttctctt
      721 gtttttcacc gtacatacgc tccagaccgt catgcatgat gacagcaact tcgtaagcgt
      781 aagccgggtc gtaagagata cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat
      841 cttcgtgctg cagaccttcg ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga
      901 agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccaatacgc tggaagccga
      961 acatcgagta atagatgtag aacgggatca tcggcagatt gttggtgctg taagaggtcg
     1021 ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc agaatctgac
     1081 ctttctcgtc ttctttatag taagcaacct gctcgcggtc ctgcggggtg tactgctgac
     1141 cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt
     1201 cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc atcacgttca
     1261 gagcacgaac gaaagcgata gtggtagaga tctctttgct ctgctcttcc aacagcgcgc
     1321 cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca
     1381 gataaccgtg cagtttctga cgctgagcgt gcagataggt atgctcttca gaaccttccg
     1441 ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt
     1501 cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac
     1561 cttcagccgc gtcgcccatg ccgtaacctt taatggtatg agcaaggatt actgtcgctt
     1621 tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac
     1681 cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag
     1741 gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtctggt
     1801 agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca
     1861 gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca gcaccttcga
     1921 agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct
     1981 gcaggttaca gttgataacg aagaccaggt tatccagttt ttcacgggta gcgatggtga
     2041 tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct
     2101 gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa
     2161 tagcaccaat cggacccaga cccatagata cggtcgggaa ctgccagaat tccggcatca
     2221 gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca
     2281 gctgctcctg agtcagacga ccttccagga aagcacgagc gtacacgccc ggggagatgt
     2341 ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt
     2401 taaagcacac atcataaatg gttgcggaag actggaagga cgccatgtgg ccgcccagtt
     2461 cgagatcttt tttcgacgca cgcagcaccg tcatgatggc gttccagcgg atagctgaac
     2521 gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg
     2581 tgttgatgta gttgctgata ccggtgcctg cggctacgtt gacaccgcct ttgcgggctt
     2641 cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg
     2701 attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt
     2761 ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt
     2821 gaactctcgc cggaaagctc aataagacag gttctacgtt tagttgccgc gcttttatat
     2881 gcgcttgatt tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt
     2941 tgctccagac gacgcagaga acgctcacgg cggctctctt cacgacttct gtcgagcaaa
     3001 atttcttcga taaaagccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg
     3061 gccataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag catctcgcga
     3121 cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg ctccatacac
     3181 cttagcagat gaagcagaac cacattgtgg gccgcttcag tgacagcaat ctgatactgg
     3241 agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagttc tatggcgtga
     3301 tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg
     3361 gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgactc aggatggtcg
     3421 gagagcagct ccaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg
     3481 ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc
     3541 aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg gagtttttcg
     3601 cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat cacatcggag
     3661 agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccgaa
     3721 gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta ttcaccttat
     3781 gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac ttgacatata
     3841 taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa ggagtttttt
     3901 aacgattatt aaactataaa aatccgaatt gaaccgattc acttaccaat tttgtgattt
     3961 ttaattcaat taaaacgaat ttaaattcat tctacatatt gagaggggtt gaggctgagc
     4021 tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc
     4081 gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct
     4141 ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt
     4201 agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc
     4261 agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag
     4321 ggaccgggtt attcctaggt agcgcctccg taatacagtc cgcagggcca gggattatcc
     4381 tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg
     4441 tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt
     4501 ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt gccatggcag
     4561 agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatccca acctgggttt
     4621 ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg
     4681 gcgagatgga gttctggttt gccattatca aagttattgc ggtagtagcg atgatcatct
     4741 tcggcggctg gctgctgttc agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt
     4801 gggatcaagg tggtttcctg ccgcacggct tcaccggact ggtgatgatg atggcgatta
     4861 tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc
     4921 cggagcaaag tatcccgaaa gcaactaacc aggttatcta ccgcatcctg attttctata
     4981 ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca
     5041 gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca
     5101 tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta
     5161 tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac
     5221 gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg tgcgtactga
     5281 ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg gtatctgcac
     5341 tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc gccaagcagg
     5401 aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc
     5461 tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg
     5521 tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa
     5581 ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca gggtgagggc
     5641 aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac cacgaccacg
     5701 aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt atcgacatgc
     5761 ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg
     5821 aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc gatcaaggca
     5881 tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg
     5941 ccggactgta aacgatgatg cggcgtctga tatcgcgcca gccagtattg ccatgcacgc
     6001 aaaaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat cagccagaac
     6061 agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa
     6121 tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc
     6181 gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga
     6241 cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag tagcgtggga
     6301 acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata
     6361 atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca
     6421 agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc
     6481 agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcacacga aaccgtgccc
     6541 aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca
     6601 tgttattagc gatatcggga tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt
     6661 gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc ccaatagaaa
     6721 aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat gcacgtttat
     6781 cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc
     6841 gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata
     6901 atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca
     6961 gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg
     7021 cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata
     7081 cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat
     7141 gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct
     7201 cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc
     7261 caacaatgac attacggcaa aacttttacc ggaaaattct cgctctcatg ccacggtgat
     7321 cacccgcgag aatggcgtat tttgcggcaa acgctgggtt gaagaggtgt ttattcaact
     7381 ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaacgccaa
     7441 tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc
     7501 gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt
     7561 actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg gcctgcgttc
     7621 agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga
     7681 tgccttcctg atcaaagaaa atcatattat tgcctccggc tcagtgcgcc aggccgtcga
     7741 aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga
     7801 acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga
     7861 acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa
     7921 cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg
     7981 tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct aattgctggc
     8041 agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc
     8101 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc tgctttcccg
     8161 tttcgttttt tgcccctcgc ttgtgccgat ggcgtttgcc aaagtagcgc caaccaaatc
     8221 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
     8281 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
     8341 aagctgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
     8401 gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc
     8461 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
     8521 ggcaagaaag tctcaatggg ctaagcgtcg tcatgacgcc aggttgggat aacgcaaacg
     8581 gcgtcaccgg ctgggcgcgc aactgcaata tccaaagtga cagcgcattg cagcaagcct
     8641 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
     8701 tcacggccct gtgcctgcgt tatcagggag tcttgctgga tgccagcgaa gaagtggttc
     8761 atgttgcggt ggtcgatgcc ccctcacatg agttgctgga cgcattgcat ttcgctacca
     8821 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca
     8881 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctga
     8941 ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg
     9001 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
     9061 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg
     9121 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
     9181 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac
     9241 agcaggtgag ccaggcactg gatgtcaaca cgcttggaat gcagccgtta caactggcgg
     9301 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
     9361 gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaataccgct gacattaata
     9421 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc
     9481 atccgcgtgc cggactcacc tttcagggcg tgttgcgtgc gttattgcgc caggatcctg
     9541 acgtcatcat gatcggagag atccgcgatg gcgaaacagc agagatcgct attaaagcgg
     9601 cgcaaactgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg
     9661 tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa
     9721 tagcccagcg tctggtacgc aaactttgcc cacattgtcg ccggcagcaa ggggagccca
     9781 tccacattcc agacaatgta tggccatcgc cgctgcccca ctggcaggca cccggttgta
     9841 tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc
     9901 cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac
     9961 gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtagag caaggcttaa
    10021 ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg
    10081 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc
    10141 cgtactttac tgcttatggc actacagcaa cagatggtta ccccactaag cctgaagcga
    10201 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
    10261 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag
    10321 catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc
    10381 attgcctttt ccaatgcctt attaccctgg tcagaggtat ttccgccgct ctatcaggcg
    10441 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcat
    10501 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc
    10561 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag
    10621 tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg
    10681 acgctggcag actttagtgg cgaatggggc tggctgctgg tgttgttcgg ctttctgctg
    10741 gcgatagcca ataagttgct gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg
    10801 ctgttacgca tcccgattat gggttcactg atgaggggac aaaaactcac gcagatcttt
    10861 acgattctgg cgctgacaca aagtgcaggc attacttttt tacagggcgt agagagcgtc
    10921 agagaaacaa tgcgctgccc gtactggatg caacttctga cacaaatcca gcacgatatc
    10981 agtaacggtc atcccatctg gctggcgcta aaaaataccg gtgagtttag cccgctctgt
    11041 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc
    11101 gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa
    11161 ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg
    11221 ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca
    11281 cgccagcgtt gggagattaa aggttgttga aaatgcggtt ttcttgttcc agcacacgaa
    11341 taaacgtggt gcgcttggtc agctctttca ggcgtgaagc cccaacgtat gtacaagctg
    11401 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
    11461 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
    11521 cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
    11581 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc
    11641 cgccaaaggc tttcgccaca tcgcccggcg tggtgcagcc accatcgctg acgatcattc
    11701 cgcccagacc gtgcgcagca tcggcacatt cgattaccgc agaaagttgc ggataaccga
    11761 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
    11821 cggcacctga gaggataagc tcctcacaca tttcaccagt cactacgtta ccagcacaaa
    11881 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
    11941 aaccattcgc cacgtcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt
    12001 tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat
    12061 cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag
    12121 cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca
    12181 tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt
    12241 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
    12301 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
    12361 tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc
    12421 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
    12481 tgagcgaacg tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat
    12541 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
    12601 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
    12661 gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat
    12721 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
    12781 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
    12841 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg
    12901 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
    12961 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc
    13021 gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc
    13081 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
    13141 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
    13201 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
    13261 agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
    13321 atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
    13381 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga
    13441 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc
    13501 cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
    13561 gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
    13621 cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc
    13681 aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca
    13741 gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt
    13801 cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt
    13861 ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct
    13921 gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
    13981 cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga
    14041 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    14101 aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc acacgtaggc
    14161 ctgataagac gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga tgcggcg
//
LOCUS       DACAPB010000119        14153 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.119, whole genome shotgun sequence.
ACCESSION   DACAPB010000119 DACAPB010000000
VERSION     DACAPB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.119"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..272)
                     /gene="yebF"
                     /locus_tag="IT904_003139"
     CDS             complement(<1..272)
                     /gene="yebF"
                     /locus_tag="IT904_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAP3192882.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPL"
     gene            complement(339..629)
                     /gene="yebG"
                     /locus_tag="IT904_003140"
     CDS             complement(339..629)
                     /gene="yebG"
                     /locus_tag="IT904_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAP3192883.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            763..1941
                     /gene="purT"
                     /locus_tag="IT904_003141"
     CDS             763..1941
                     /gene="purT"
                     /locus_tag="IT904_003141"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAP3192884.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVNAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(1997..2638)
                     /locus_tag="IT904_003142"
     CDS             complement(1997..2638)
                     /locus_tag="IT904_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP3192885.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(2675..4486)
                     /gene="edd"
                     /locus_tag="IT904_003143"
     CDS             complement(2675..4486)
                     /gene="edd"
                     /locus_tag="IT904_003143"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAP3192886.1"
                     /translation="MNPQMLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDGNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(4721..6196)
                     /gene="zwf"
                     /locus_tag="IT904_003144"
     CDS             complement(4721..6196)
                     /gene="zwf"
                     /locus_tag="IT904_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP3192887.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            6534..7403
                     /locus_tag="IT904_003145"
     CDS             6534..7403
                     /locus_tag="IT904_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP3192888.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            7531..8973
                     /locus_tag="IT904_003146"
     CDS             7531..8973
                     /locus_tag="IT904_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase II"
                     /protein_id="HAP3192889.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(9104..10075)
                     /gene="lpxM"
                     /locus_tag="IT904_003147"
                     /gene_synonym="msbB"
     CDS             complement(9104..10075)
                     /gene="lpxM"
                     /locus_tag="IT904_003147"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAP3192890.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(10195..11517)
                     /gene="mepM"
                     /locus_tag="IT904_003148"
     CDS             complement(10195..11517)
                     /gene="mepM"
                     /locus_tag="IT904_003148"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAP3192891.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(11533..12465)
                     /gene="znuA"
                     /locus_tag="IT904_003149"
     CDS             complement(11533..12465)
                     /gene="znuA"
                     /locus_tag="IT904_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAP3192892.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            12544..13299
                     /gene="znuC"
                     /locus_tag="IT904_003150"
     CDS             12544..13299
                     /gene="znuC"
                     /locus_tag="IT904_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAP3192893.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVHAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            13296..14081
                     /gene="znuB"
                     /locus_tag="IT904_003151"
     CDS             13296..14081
                     /gene="znuB"
                     /locus_tag="IT904_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAP3192894.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAVYDTPAGPSVVLCAALLFILSMMKKQAS"
BASE COUNT         3328 a         3669 c         3552 g         3604 t
ORIGIN      
        1 agcggtactg accatttatc atctttaccc tgaatgtcct gcaaactgac ccaagccacg
       61 ggatcggcct gaccgacaat tttttgatca tctgcccaac gcgccacgcg attttgttga
      121 taatcacgtt ttacgctcgc ggcaattccg gcagcatcca gaccttcaca ctttgggaaa
      181 gtgaccgact tgctggtttc attattggca gcgaaaactg atgcgcaggc agaaaccaac
      241 aacagcccta aaaacgcccc tctttttttc atgtttttct ccatagcaca atgattcagg
      301 agaaagcatg gtacaaattg tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct
      361 tcctcttcgg attcagcacc gacgacctgt ggggacggta atttgccggt tttcagaatg
      421 gtgctcagca catctttttg ttctgccagc catagcgaaa gggcttcacg ttgctcgtct
      481 tccatttgca ctggagaatt tgtcagccag gtgtcaagca gatccgccgt atcaagcatt
      541 ttgtcataag catcggcttc ctttttgctg gtaaacgaca ttttctcttc gccctcacga
      601 atgactacgt atttaacttc aaccgccatt tgcagcctct cataataact gtgattttat
      661 acagtatatt tcttttcggt tgagaaatca acatcagcaa taaagacaca cgcaaacgtt
      721 ttcgtttata ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt attaggcact
      781 gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa
      841 gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg ctgtcgatcg ctattccgac
      901 gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta atatgcttga tggtgatgca
      961 ttacgccgtg tggttgaact ggaaaaacca cattatatcg tgccggagat cgaagctatt
     1021 gccaccgata tgctgatcca acttgaagag gaaggactga atgttgtccc ctgcgctcgc
     1081 gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga agagctgcag
     1141 ctgcccactt ccacttatcg ttttgccgat agcgaaagcc ttttccgcga ggcggttgcc
     1201 gccattggct atccctgcat tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg
     1261 tttattcgct ctgcagagca acttgctcag gcatgggaat acgctcagca aggcggtcgc
     1321 gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga aattaccctg
     1381 ctaaccgtca acgcggtgga tggcgtccat ttctgtgcac cagtaggtca tcgccaggaa
     1441 gatggcgact accgtgaatc ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt
     1501 gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg gttatgggtt gtttggtgtc
     1561 gagctatttg tctgtggtga tgaggtgatt ttcagtgagg tctcccctcg tccacatgat
     1621 accgggatgg tgacgttaat ttctcaagat ctctcagagt ttgccctgca tgtacgtgcc
     1681 ttcctcggac ttccggttgg cgggatccgt cagtatggtc ctgcagcttc tgccgttatt
     1741 ctgccacaac tgaccagtca gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca
     1801 gatttgcaga ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt
     1861 gcgctggcga ccgctgagag tgttgttgac gccattgaac gcgcgaagca cgccgccgga
     1921 caggtaaaag tacagggtta aacccggcaa aaaaacgcta caaaaatgcc cgatcctcga
     1981 tcgggcattt tgacttttac agcttagcgc cttctacagc ttcacgcgcc agcttagtaa
     2041 tgcggtcgta atcgcccgct tccagcgcat ctgccggaac cagccaggaa ccaccgatgc
     2101 acagcacgct tttcagcgcc aggtagtcac ggtagttagc cggagaaata ccacccgtcg
     2161 ggcagaaacg gacctgggag aacggacccg cgatcgcctg cagggctttc acgccgccgt
     2221 tagcttcagc cgggaagaat ttgaactctt tcaaaccgta gtccataccc agcatcagtt
     2281 cggaaacagt gctgatcccc ggaatcagag gaatagtccc ttcggtagca gctttcagca
     2341 gcggctcggt cagacccggg ctaattgcga actgtgcacc cgcttcagtg acttctgcca
     2401 gctgctgtgg attcagcacc gtaccggcac ccacaatcgc ttcaggcact tctttggcga
     2461 tagcacggat agcgtcaact gcacactcgg tacgcagagt cacttccaga acgcgcaccc
     2521 caccagcaac caacgctttt gccatcggca ccgcgtgttc cagttttttt accacgataa
     2581 ccggtacaac cgggccggtg gtcaggattg attctgcact tgttttccag tttttcatca
     2641 gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag gttgcgccct
     2701 gttcggcacc ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct gttcccacgc
     2761 gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc cagttccgct tcgtctacca
     2821 gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg cgcacttttg
     2881 ccagcagccc gccatcgtag gcttctggtg ttacgtggat agctgacggc actttacctg
     2941 aagcgccgga gagtcgtcca tcggtaacta acgcaatttt gaaacaccgg tccaataata
     3001 caccaagtgg cggcatgagt ttatgtaatt ctggcattcc gttcgctttt ggcccctgat
     3061 gacggacaac aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt
     3121 catgctggct ttcaaaaaca accgctggcg cttcaatcac ctggttctca accggcacgg
     3181 cagaggtttt cataaccgca cggcccaggt taccgcttaa cacttttgtc ccaccatgat
     3241 gagagaaagg ttgttcgaag gaagcgatca cattgccgtc gagtgatttt tccgcccctt
     3301 cccgccagtc cagttcacca ttattcagcc atggttcaag cgtataacga gacagaccaa
     3361 agcctgccac cgtattgaca tcttcatgca gcagacctgc tttgagcagt tcacgcacca
     3421 gaaccggtac gccacctgcc gcctggaagt ggttaatatc ggccggaccg ttcgggtaga
     3481 gacgtgccat cagcggtaca acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa
     3541 taccggccgc gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg
     3601 ccagcagtgc aacaataccg ttcaccacca ctttctcatc gatcatctta ccgatcggca
     3661 tccattcatt accattaccg gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag
     3721 catcgcgcag cggagagtcc ggatgaacaa aagaagagcc tggcaactgc atccccataa
     3781 actccaccac catctggttg gtgttggcag taccgtagaa agtacatgtt cccggcgcat
     3841 gataagacgc ggcttctgac tccagtaggg ccatgcggtc cactttacct tcggcataaa
     3901 gctggcgaat acgcactttt tctttatttg gcaaaccgct tgccatcggt ccagacggta
     3961 caaacaccgc aggcaaatga ccaaacgaca gggctgccat cgtcagaccc gggacaatct
     4021 tgtcgcacac cccgaggaac agagcaccat caaacatgtt atgggacagc ccgaccgccg
     4081 cagacatcgc tatcacttcg cggcttagca gcgacaattc cattccatcc tgcccctggg
     4141 tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat
     4201 gcagggcttt acgaatgatt tctggatagt gttcataagg ctggtgcgcg gagagcatgt
     4261 cgttatagga ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa gaggctttgt
     4321 cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat
     4381 gaacggtcga cgttttcgct tgttctatcc gggcgagata agcagagcga gtctcgcgcg
     4441 aacgttcaat gattcgattt gttacgcgta acatttgtgg attcataaag gctcctgaaa
     4501 ttgagttgtc agagcaggat gattcacaac gcgtttcatt cagaggattt atgagtgaaa
     4561 cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc
     4621 gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt tctacaatct gcgcaagatc
     4681 atgttaccgg taaaataacc ataaaggata agcgcagata ttactcaaac tcattccagg
     4741 aacgaccatc acgggtaatc atcgccaccg aggcaacggg tccccaggtt ccggcctgat
     4801 acggtttcgg cgcatcattg tccatcgccc acgcctcagt aatggagtct acccatttcc
     4861 aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg aataccacgc atggtttcca
     4921 gcagcaaacg ttcataggca tccgccagat gcgtctgatt aaaggtttct gaatagctca
     4981 gatctagctt ggtgatttgc aggttatgtt tgtggtcaag gccaggaact ttattcagta
     5041 cctggatatc cacgccttca tcaggttgca gacggatagt cagtttattc tgcggcagat
     5101 cctgccacga ttctttaaac agattcagtt caggtgtttt gaaatagacc acgacttcag
     5161 aacatttggt cggcagacgt ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc
     5221 agttatcaat gtcgacgcgg atcgccacga aagtttctgt attgctgctc ttgttcgcgc
     5281 cctcttcttc cagatatccc ggcacttttt tgccctgggc gaagcccgca gtatattgcc
     5341 cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg gcgcagagac ttcagtactt
     5401 tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga cggcggagac atcgcaatca
     5461 tgcaaagaat ttgcagcagg tggttctgga tcatgtcgcg catctgaccg gctttatcaa
     5521 aatagcccca gcgcccttcg atccccactt cttctgccac ggtaatctca acatgatcaa
     5581 tggtgcgatt gtcccagtta ttcacaaaca gggagttagc aaaacgcagc gccaacaggt
     5641 tcagcaccgt ttctttacca agatagtggt cgatacggta aacctggcac tcctcgaagt
     5701 attcgccaac ctgatcattg atttcctgcg aggtcgccag cgacgtcccc agcggtttct
     5761 ccatgactac gcgtgccggt ttagcattca gttttgcctc gccaagccct ttgcaaattg
     5821 cgccaaaagt gctgggcggc atggcaaagt agttaatggt gatacgattt ttttgatcca
     5881 gcatcgcgcc gagacggctg aatgcagcag tgtcattgac atcgagatta caaaaatcca
     5941 gacgtgcact cagggtgtcc cataaacctt catcaatggt ttctttcatg aaagtttcga
     6001 gcgcctcgcg gacaactttg gtatatgccg ctttatccca gtcagcacgc cctacgccga
     6061 taatccgggt gtccgggttg agctgaccgg ctttttccag ttgatacagg gaaggcagca
     6121 atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac caggtcacag gcctgggctg
     6181 tttgcgttac cgccatgtca ttctccttaa gttaactaac ccggtactta agccagggta
     6241 tacttgtaat tttcttacgg tgcactgtac tgcttttacg agcttgcgaa aactgtaaac
     6301 gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt gcggcaaaac tgataaaaaa
     6361 atcatcgttt tccattgggt aaaaatctga cactgatcat gttatgaaaa aaaataacaa
     6421 cttttttatc tgcttttgtc attaacacgg cacacaggcg taatatctga caaaactgca
     6481 tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga gtctctttac atcatgaata
     6541 tgctggaaaa aatccagtct cagctggaac atttgagcaa atcagagcgc aaagttgccg
     6601 aggtcattct ggcttcgccc gataacgcga tccattcgag tattgctgct atggcactgg
     6661 aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg cagcatggac acgcgcggtt
     6721 ttcctgattt taaacttcat ctggcacaga gtctggcgaa tggcactccc tatgttaatc
     6781 gcaatgtcaa tgaagatgac agcgttgaat catacacagg gaaaatattt gagtccgcaa
     6841 tggcaacgct tgatcatgtc cgtcattcac tggataaatc tgccatcaac cgcgccgtcg
     6901 acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt aggctcttca gccgccgttg
     6961 cccacgatgc gatgaataag ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata
     7021 tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt
     7081 ctcacactgg aagaacaaaa aatctggtcg agctggcgca gctggcacgc gaaaacgacg
     7141 ccatggtgat tgccctcacc tctgcgggta ccccgctcgc ccgggaagca acgctggcaa
     7201 ttaccctcga cgtaccggaa gatactgaca tttatatgcc catggtttct cgacttgcac
     7261 agctgaccgt gatagatgtg ctggcgacag gatttacttt gcgacgcggt gcaaaattca
     7321 gagataactt gaagcgggtc aaagaagcgc tgaaggaatc gcgttttgat aagcagttac
     7381 ttaatttaag tgacgatcgc taaaaacgac tgtcactgtc ctaatcttat acgacatccg
     7441 aatgagatta atttatcgcc atcgcggcgt tatttcattc ggatttcatg ttcaagcaac
     7501 acctggttgt ttcagtcaac ggagtattac atgtccagaa ggcttcgcag aacaaaaatc
     7561 gttaccacgt taggcccagc aacagatcgc gataataatc ttgaaaaagt tatcgcggcg
     7621 ggtgccaacg ttgtacgtat gaacttttct cacggctcgc ctgaagatca caaaatgcgc
     7681 gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac
     7741 ctccaggggc ccaaaatccg tgtatccacc tttaaagaag gcaaagtttt cctcaatatt
     7801 ggggataaat tcctgctcga cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc
     7861 ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg gtgacatcct gctgctggac
     7921 gatggtcgcg tccagttaaa agtactggaa gttcagggca tgaaagtgtt caccgaagtc
     7981 accgtcggtg gtcccctctc caacaataaa ggtatcaaca aacttggcgg cggtttgtcg
     8041 gctgaagcgc tgaccgaaaa agacaaagca gacattaaga ctgcggcgtt gattggcgta
     8101 gattacctgg ctgtctcctt cccacgctgt ggcgaagatc tgaactatgc ccgtcgcctg
     8161 gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt
     8221 tgcagccagg atgcaatgga tgacatcatc ctcgcctctg acgtggtaat ggttgcacgt
     8281 ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg gcattcagaa agcgttgatc
     8341 cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga cccagatgat ggagtcaatg
     8401 attactaacc cgatgccgac gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat
     8461 ggtactgacg ctgtgatgct gtctgcagaa actgccgctg ggcagtatcc gtcagaaacc
     8521 gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct
     8581 aaacaccgtc tggacgttca gttcgacaat gtggaagaag ctattgccat gtcagcaatg
     8641 tacgcagcta accacctgaa aggcgttacg gcgatcatca ccatgaccga atcgggtcgt
     8701 accgcgctga tgacctcccg tatcagctct ggtctgccaa ttttcgccat gtcgcgccat
     8761 gaacgtacgc tgaacctgac tgctctctat cgtggcgtta cgccggtgca ctttgatagc
     8821 gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga taaaggttac
     8881 ttgatgtctg gtgacctggt gattgtcacc cagggcgacg tgatgagtac cgtgggttct
     8941 actaatacca cgcgtatttt aacggtagag taagtacgtt gccggatgcg gcgaaaacgc
     9001 cacatccggc ctacagttca atgatagttc aacagatttc gaatattctg aagcaaactt
     9061 gaacttatca tcaggcgaag gcctctcctc gcgagaggct tttttatttg atgggataaa
     9121 gatctttgcg cttatacggc tggatttcgc ccggtttgcg agttttcagc aattttagta
     9181 tccaggtgta ttgttctggt cgcggaccaa caaaaatctc gacttcttca ttcatccgcc
     9241 gcgcaatcgt atgatcatcc gcctctaaca gatcatccat cggtgggcgc acctgaatcg
     9301 tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca
     9361 ctttcatcaa acgaccaatc gcgggcaacg tcgctttata ggtggcaaag aaatccacaa
     9421 attcgctgtg ctctgggcca tgatcctgat cgggtaaata atatccccag tacccctgac
     9481 gtaccgactg gatgaatggt ttaataccgt catttctcgc atgcagacga ccgccaaagc
     9541 gacgacgcac cgtgttccag acataatcaa aaaccgggtt gccctgatta tggaacatcg
     9601 ctgccatttt ctgcccttgc gaggccatca gcatggcagg aatatcgacg gcccaaccgt
     9661 gcggcaccag aaagataact ttctcgttat tacgccgcat ctcttcgatg atctccagcc
     9721 cttgccagtc aacgcgcggc tgaattttct ccggcccgcg tattgccaac tcagccatca
     9781 ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac aatcgcttcg cgttcagctt
     9841 cactacgttc tggaaagcag agcgacagat tgattaacgc acgacggcgt gagctttttc
     9901 ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg atcacggaac tttggcggcg
     9961 ttaaagcgat acccgccatc gctgctacgc ccagccatgc tccccagtag cgcgggtggc
    10021 gaaaggattt atcaaactca ggaatgtatt cgctattatt ttttttcgtt tccatgcttt
    10081 tccagtttcg gataaggcaa aaatcaatct ggtgatagtg tagcggcgca acttgccccg
    10141 caccaaataa aaaaagccgg tactgactgc gtaccggctg cgaatggatg ttaattaatc
    10201 aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg aattcgcgac gatcggagcc
    10261 ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg tttacggcct gctggtttat
    10321 ccatacttca tagtgcagat gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat
    10381 acggtcgcca cgtttcacct tctgtcccgg tttcaccaga atcttgcgca agtgcatata
    10441 acgcgtggtg tagctgcgac catgacgaat agccacataa taacctgctg cgccactgcg
    10501 tttggcaacc accacttcac cgtcacccac tgaaagcact ggcgtaccct gcggcatggc
    10561 gaaatcaaca cctctgtgtg gtgcaacgcg accggtcacc ggattagtac gacgcgggtt
    10621 aaagttagac gagatacgga actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag
    10681 accagtaccg ttacgatcgt agaatttgcc atcttcagcg cggatagcgt aataatcttt
    10741 accttctgaa cgcaaacgta cgcccagcag ctggctttgc tcacgtttac catcaagcat
    10801 ttctcgagac attaacaccg caaattcatc gccttttttc agtttgcgga aatccatttg
    10861 ccactgcatg gctttaatca ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc
    10921 gctggcaaca aagcttcccc cgacggtacc tttcagcaga ttgttaaccc actctccttg
    10981 ctgcatttcg ctggtcattt taaaaccgtt agcggcagta cggtcatagg ttcgggtttc
    11041 acgacgagac acttcccagg tgaggcgctg cagttcgccg tccgcggtta atgtccagga
    11101 gagttgttga ccgattttca ggttacgcaa ttctttgtcg gccgcagcca gttgggtgat
    11161 atcacccata tcaataccat actgattgag aatgctgctt agcgtatcgc cagtggaaac
    11221 aacatattca tgcacgcccg cttcaccggc gattttgtca tccagttcgt cctggggaat
    11281 ggcttcatct tcttgtgcag cttgatcaat cggctcactg gcttcaggta agagcgaacg
    11341 aatttcgttc tgttccagct caatggtttt gacaattggc gtggcatcac ggtgataaac
    11401 ataaggccgc cagacagcga cggccagagt aagaacggtg agcgacccca acataacgcg
    11461 gtgtggtcgc ggtaaattat taaacgccag ggcgacagag cgggctatct gttgcacgta
    11521 atcacttcct cattaatctc ctttcaggca gctcgcatac tggttggcta attgactcag
    11581 gaattctgaa tagcttgttt tacccagttt gatattcgtc cccaggggat ccaacgttcc
    11641 catacgaacg gatgtccctc gtgcgacgct ctcaacgacc gctggcctga actgtggctc
    11701 agcaaaaacg caggttgctt tttgctcaac caactgtgtt cttatttcat gtaaacgctg
    11761 cgcgccaggt tgaatctcag ggttaacggt aaaatgacca agcggtgtca gtccaaactg
    11821 tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa cctttcccct tgagcggcgc
    11881 gagctcgtta ccaacctgcg tttcggttga ggctaattgt gcctcaaaat ccttcaggtt
    11941 ggcgtcaagt ttggctcgac tttgcggcat aagttccact aattttccat ggattgcaac
    12001 cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg
    12061 gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca tcatcgccgt gaatactttt
    12121 catcagtagc ggtttcacat cttcaagctg cgcaatcgtt acctgcttcg ctcctggtaa
    12181 tttgcttacc ggtttttgca taaacgcttc catctccggg ccaacccaaa cgactaagtc
    12241 cgcgttctgt aagcgtttta catccgatgg gcgcagtgaa taatcatgtt ctgaagcgcc
    12301 gtcaggaagt aaaacctctg tttctgttac cccatcagca atggcagaag cgatgaaccc
    12361 aacgggttta agcgaagcga caacggcggc atctgctgcc tgtgttgcac ctccccagag
    12421 agcggcggat aatgctgcga aaagaagcgt ttttttatgt aacataatgc gaccaatcat
    12481 cgtaatgaat atgagaagtg tgatattata acatttcatg actactgcaa gactaaaatt
    12541 aacatgacaa gtctggtttc cctggaaaat gtctcggttt cttttggcca acgccgcgtc
    12601 ctctctgatg tgtcgctgga acttaaacct ggaaaaattt tgactttact tgggccaaat
    12661 ggcgcaggta agtcgacact ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg
    12721 gttatcaagc gcaacggaaa actgcgcatc ggctatgtac cgcagaagct gtatctcgac
    12781 accacgttgc cactgaccgt aaaccgtttt ttacgcttac gccctggtac acataaagaa
    12841 gatattttgc ctgcactgaa acgtgttcat gccgggcacc tgattaacgc accgatgcaa
    12901 aagctctctg gtggcgaaac gcagcgtgta ctgttagcgc gagcattgtt aaatcgcccg
    12961 caattattag tgctggatga acccactcaa ggcgtggatg taaatggcca ggtggcgtta
    13021 tatgacctta ttgaccaact gcgtcgtgaa ctggattgtg gcgttttaat ggtttctcac
    13081 gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt gcctgaatca ccacatttgt
    13141 tgttccggca caccggaagt tgtttccctg catccggagt ttatttcaat gtttggtcct
    13201 cgtggtgctg aacaactggg tatctatcgc catcatcata accatcgtca cgatttacag
    13261 ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat tgaattatta tttcccggtt
    13321 ggttagccgg gatcatgctc gcctgtgccg cgggtccgct gggttcgttt gtagtctggc
    13381 gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc attacttggc gtcgcgtttg
    13441 gtttgttgct ggacgtgaat ccattctatg cggtgattgc cgttacgctg ctgctggcgg
    13501 gcggtctggt atggctggag aagcgtccac agctggcgat cgacacgtta ttagggatta
    13561 tggcgcacag tgccctgtcg ctgggcctgg tggtcgtgag tctgatgtct aatattcgtg
    13621 ttgatttgat ggcttacctg ttcggtgatt tgctggcagt gacgccagaa gatctcatct
    13681 ctattgcgat tggcgtggtc atcgtggtgg ctattttgtt ctggcaatgg cgcaatttgc
    13741 tgtcaatgac gataagcccg gatctggcgt ttgttgatgg tgtgaaatta cagcgagtga
    13801 aattgttgtt gatgctggtg acggcattga cgattggtgt agcgatgaaa tttgtcggtg
    13861 cgttgattat tacttcgttg ctgattattc ctgctgctac tgcgcgtcgc tttgcccgca
    13921 cgccggaaca gatggctggt gtcgctgttt tggtggggat ggtggcagtg actggcggtt
    13981 taaccttttc cgcggtttac gatacgccgg cgggtccgtc ggtggtgcta tgtgcggcac
    14041 tgttatttat tctcagtatg atgaaaaagc aggccagcta atctatcgct gaacacattt
    14101 gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg ctatctctgg ctg
//
LOCUS       DACAPB010000120        14119 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.120, whole genome shotgun sequence.
ACCESSION   DACAPB010000120 DACAPB010000000
VERSION     DACAPB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.120"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(71..2575)
                     /gene="copA"
                     /locus_tag="IT904_003152"
     CDS             complement(71..2575)
                     /gene="copA"
                     /locus_tag="IT904_003152"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAP3192895.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKHLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLHNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            2837..3769
                     /gene="glsA"
                     /locus_tag="IT904_003153"
     CDS             2837..3769
                     /gene="glsA"
                     /locus_tag="IT904_003153"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP3192896.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVANQLGYNVFKG"
     gene            3772..5064
                     /locus_tag="IT904_003154"
     CDS             3772..5064
                     /locus_tag="IT904_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3192897.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            5189..5596
                     /locus_tag="IT904_003155"
     CDS             5189..5596
                     /locus_tag="IT904_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAP3192898.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(5597..6055)
                     /locus_tag="IT904_003156"
     CDS             complement(5597..6055)
                     /locus_tag="IT904_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAP3192899.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(6052..6969)
                     /locus_tag="IT904_003157"
     CDS             complement(6052..6969)
                     /locus_tag="IT904_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAP3192900.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            7115..7792
                     /gene="fetA"
                     /locus_tag="IT904_003158"
     CDS             7115..7792
                     /gene="fetA"
                     /locus_tag="IT904_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAP3192901.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            7779..8558
                     /gene="fetB"
                     /locus_tag="IT904_003159"
     CDS             7779..8558
                     /gene="fetB"
                     /locus_tag="IT904_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAP3192902.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(8621..9475)
                     /gene="cnoX"
                     /locus_tag="IT904_003160"
     CDS             complement(8621..9475)
                     /gene="cnoX"
                     /locus_tag="IT904_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAP3192903.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(9536..10345)
                     /gene="ybbO"
                     /locus_tag="IT904_003161"
     CDS             complement(9536..10345)
                     /gene="ybbO"
                     /locus_tag="IT904_003161"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP3192904.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(10335..10958)
                     /gene="tesA"
                     /locus_tag="IT904_003162"
     CDS             complement(10335..10958)
                     /gene="tesA"
                     /locus_tag="IT904_003162"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAP3192905.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            10929..11615
                     /locus_tag="IT904_003163"
     CDS             10929..11615
                     /locus_tag="IT904_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3192906.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            11612..14026
                     /locus_tag="IT904_003164"
     CDS             11612..14026
                     /locus_tag="IT904_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3192907.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         3098 a         3781 c         3882 g         3358 t
ORIGIN      
        1 cgccgcatcc ggcagtcatg cgtcgatgcc aaatgcgcca ccctaaagca gcgcatccgc
       61 aatgatgtac ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg
      121 taatcgacga gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtga
      181 acggccacaa aataccggcg gcgaccggaa taccgatact gttgtagata aacgcaccga
      241 gcaggttctg cttcatgttg tgcagcgttg cgcgggaaat agcgagcgca tccgcaacgc
      301 ccatcaggct atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc
      361 cacccatcgc aatgccgaca tccgcctgag ccagcgctgg cgcgtcgtta atgccgtcgc
      421 ccaccattgc cacctgacgt ccttcacttt gcagatgttt gatcgcttcg gctttaccgt
      481 ccggcagcac cccggcgatc acctcatcaa tccctgcttc tttggcgatc gcattggcgg
      541 tggttgggtt atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca
      601 gcgccgccac gctatcacta cgcaatggat cgcgtacagc cagcagggct actgctttcc
      661 cgtcaatcgc cagcagcaca ggcgttgctc cttgcgatgc ctgagctgaa atatcggctt
      721 caatcgcttt agtatcgact tgctgatcat ttaacaacgc ctgattgccc agcaataacg
      781 catgaccttc agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct
      841 gcggtagctg catatcgcct gctttatcga ggatcgctcg tgccagcgga tggctggaac
      901 cttgttccag tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaatgttt
      961 tcactgcgac aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg
     1021 tatcgagtgt actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag
     1081 ccgcccgccc gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac
     1141 aggcgataat cagtaccgtg gtggcaatta ccagggtata aacaatttgc ggtgccggac
     1201 caaagaagta ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata
     1261 cggctgagat tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca
     1321 ccatgcgaat gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca
     1381 cactgccgtc ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgtg
     1441 ggattggttc gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc
     1501 catctaccgg cacgcgatcg ccggtcgtca ggcgcagtaa catccctggc tgaacttccg
     1561 ccagcggcac gcttttttca ccctctgcgg tgaccactcg cgccgtcggt ggggttaaat
     1621 cgagtaattt ctccagcgcc ttagaagaac gctgacgtgc gcgcgcttcc agcatatggc
     1681 cgagattaat cagaccgata atcatcgcac tggcttcata gtaaagatgc cgcgcttcca
     1741 tcgggaacca ctgcggccac aggttgacgc tcatcgaata gagccacgcc acacccgttc
     1801 ccagcgccac cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc
     1861 ggtaaaaatg gccgccggcg aaaaccatca ctgccagggt tatcaggccg ataactaacc
     1921 acaagctgcg gttgtcagcg gtgaccatca tgttatcgcc gatcatcccc cagaccatta
     1981 ccgggatacc cacagccagt gcaacaattg cctgccagcg gaagcgcttc atcgtagcta
     2041 cggcggtttc ttgctggcgc tcgcggcgtt tagcgtcatc ttcaatcgct tccgcaccgt
     2101 agcccgcttt ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg
     2161 cagtacgctc cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat
     2221 tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt
     2281 catcgtcatc ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg
     2341 ggattgatga ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg
     2401 cttgtttgat ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag
     2461 tgatagacac atccgcctgc tcaacatccg ggcgctgttc aagactttct ttcacgcgtt
     2521 taacgcagtg accgcaggac aggccgtcca gggtcaggtc gatagtttgt gacataaaac
     2581 actcctttaa gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag
     2641 gtcaagaaat taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat
     2701 ttgcatcagg attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag
     2761 ttaaatcagg atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa
     2821 aaggagttaa caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta
     2881 cacccaattt cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa
     2941 tgtacctggt caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc
     3001 gggtgatagt gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct
     3061 tgcgttagaa gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg
     3121 attgcccttt aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact
     3181 ggtaaatgct ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg
     3241 ctggcagcga attttacata tccaacagca actggctggt gagcaggtag cgctctctga
     3301 tgaagtcaac cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct
     3361 gtactccgcc ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca
     3421 gtgctccacg ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg
     3481 tggtgtgaat ccgctgacgc ataaacgtgt tcttcaggcc gacaatgtgc cgtacattct
     3541 ggccgaaatg atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg
     3601 tttaccgggc aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg
     3661 aattgccgcg ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat
     3721 ggtggcatcg gtcgctaacc aactcggcta taacgtgttt aagggctgat catgatgaac
     3781 acggaaggta ataacggtaa caaacctctc ggtctatgga acgtcgtttc catcggcatt
     3841 ggggcaatgg tgggggcggg gatcttcgcg ctgctggggc aggctgcatt gctaatggaa
     3901 gcctcgacct gggtcgcctt tgcttttggc ggtattgtgg cgatgttttc cggttatgcc
     3961 tatgcgcgtc tgggggcgag ctatcccagc aatggcggca ttatcgactt ctttcgtcgc
     4021 ggattaggca acggcgtctt ttcgctggcg ctctcgttac tgtacctgtt gacgctggcg
     4081 gtgagcatcg ccatggtcgc ccgtgctttt ggcgcttatg ccgtgcagtt tttgcatgaa
     4141 ggcagccagg aggagcacct tattttgctc tacgcgttgg ggatcattgc ggtgatgacg
     4201 cttttcaact ccttaagcaa ccatgcggta gggcggctgg aagtgatcct cgtcggcatt
     4261 aaaatgatga tcctgttatt gctgattatt gccggtgtct ggtcgctgca accggcgcat
     4321 atttccgtct ctgcgccccc cagctccggt gcgttcttct cctgtattgg gataactttc
     4381 cttgcctatg cgggctttgg catgatggcg aacgcggcgg ataaagtgaa agatccgcag
     4441 gtcattatgc cacgggcgtt tctggtggcg attggcgtta ccacgttgct ttatatctcg
     4501 ctggcactgg ttttgcttag cgatgtatcg gcattagagt tagaaaaata tgccgatacc
     4561 gccgtagcgc aggctgcttc tccgctgctc gggcatgtgg gttatgtgat cgtcgtcatc
     4621 ggcgctttac tggcgacggc ttcagccatt aacgcgaacc tgttcgccgt gtttaacatc
     4681 atggacaaca tgggcagcga acgcgaactg ccgaagctaa tgaataaatc cctgtggcgg
     4741 cagagtacct ggggcaacat tattgtcgtg gtgttgatta tgctgatgac ggcggcactg
     4801 aatttaggct cactcgccag cgttgccagc gccacctttt tgatttgcta cctggcggtg
     4861 tttgtggtgg cgatccgcct gcgtcatgat attcacgcct cgttgccgat tcttatcgtt
     4921 ggtacgttgg tgatgttgtt ggtgatcgtt ggctttatct acagtctgtg gtcccagggt
     4981 agccgtgcgt tgatatggat tattggctca ctcttactca gccttattgt ggcaatggtc
     5041 atgaagcgca ataaaaccgt ataacatctc tctgtgcgca gtacttcctg tattattgtg
     5101 gtggcggtcg atattcgcac tggcaaaaaa acgtgcttga atatctgttg aaacccttta
     5161 acaaagcaca ggaggcgttg cgcgaacgat gaacatcagc gatgtagcaa aaattaccgg
     5221 cctgaccagc aaagccattc gcttctatga agagaagggg ctggtgacgc cgccgatgcg
     5281 cagcgaaaac ggttatcgca cctacacgca gcagcatctc aacgaactga ccttactgcg
     5341 ccaggcacgg caggtgggct ttaacctgga agagagcggc gagctggtga atctgtttaa
     5401 cgacccgcag cggcacagcg ccgacgtcaa acggcgcacg ctggagaagg tggcggagat
     5461 cgaacgacac attgaggagc tgcaatccat gcgcgaccag ctgctggcac tggcgaatgc
     5521 ctgccctggc gatgacagcg ccgactgccc gattatcgaa aatctctccg gctgctgtca
     5581 tcatcgggca gggtgattaa gacgagacgg ctcggatatg tagggttatc ccttctatcg
     5641 caatgacttc aacatgcgta cctgcaccga gatcctcgct ggcgctgaca ggccatgaac
     5701 tgtcaccgac gcgcatatga ccgcgcccgt tgaccagcgg agattccagc acaaaacgtc
     5761 ggccaatcag ctgctgcccg cgctggttta aatgactatc gctgtgcttt tgttcgcgca
     5821 cccgccgcga caaccatttc caccacagcc aggcggcgag cagcgtcagg atggcaaaca
     5881 ttaccccttg ccactcccaa cccagcggca ccagccagac caccaggccg gtaatcaccg
     5941 ctgccacgcc gctccacaac aaataaccat ttccgcccag catctcggct gccagcagca
     6001 aaccgccgag actgagccag aaaatatgtg gatgaacgac cattaactcc atcatggctg
     6061 agtccgtttg ttggcgctgt ctttcaccag ctcggcaatc ccggcaatcg accccatcag
     6121 gctgctggcc tctaatggca tcatcactac tttgctgtta ctggaggaac cgatctgctg
     6181 taacgcttcg gtgtatttct gcgctacgaa gtagttcacc gcctgaatat caccggaggc
     6241 gatagcttca gacaccattt tggtggcgcg ggcttctgct tcggcggaac gttcacgcgc
     6301 ttcagcctgt aaaaacgccg actgacgttc gccttccgct ttcaggattt gcgactgttt
     6361 ttcaccttcg gctttgagga tttccgcctg acggatccct tccgcttcaa gaatgtaagc
     6421 gcgtttggta cgttccgctt tcatctgcgc gttcattgaa gagataagct ctgccggtgg
     6481 gcgcacgtcg cgaatttcaa tacgggtgac tttaatcccc cacgggttgg tggcttcatc
     6541 gacaatacgc agcaggcgtg agttgatgct gtcgcgctga gagagcattt cgtcaagttc
     6601 cattgaaccc aacacggtac ggatgttagt catggtcagg ttgatgatcg ccagctccag
     6661 attgctgact tcataagccg cgcgcggcgc gtcaatcacc tgaataaaac agactgcgtc
     6721 gatggtaacg ttggcgttat ctttcgagat aacttcctgg gaagggatat cgagcacttg
     6781 ctccatcata ttgatcttgc gaccaatgcg atccataaac ggcaccacca gactgagccc
     6841 cggctgtaac gttttggtat agcgaccaaa acgttctacc gtccattgat agccctgcgg
     6901 cacgattttg acgcccgcgc cgacaatgac cagcgcgaca aaaatgagaa tcgggataaa
     6961 gataagcatc ggaaaaacct cctgttgtac cgtccataat cagcaaaatt gctgcttgat
     7021 taaacaaatt atacctgatt actgaaagag agttccccct tattcctgcg aaggataaac
     7081 tgtttttagt aaaaatcaga aaaagggaac agcgatgcag gaaaatagtc ctttgcttca
     7141 gctacaaaac gtaggatatc tggcgggtga tacgaagatt cttaataaca tcaatttttc
     7201 gctgcgtgct ggcgaattta agttaattac cggtccttct ggttgtggca aaagtacgct
     7261 gctaaaaata gttgcttcat tgatcagccc aaccagcgga acgttactgt ttgaagggga
     7321 ggatgtcagc acactaaagc cagaaatcta ccgccagcaa gtctcttact gcgcccagac
     7381 accgacgctg tttggcgata cggtatacga taatctgatc tttccctggc agatccgtaa
     7441 ccagcagcct gacccggcca tttttctcga ttttctcgaa cgcttcgctt tgccggacag
     7501 cattttgacg aaggatatcg ccgagctatc tggtggtgaa aaacaacgca tctcattgat
     7561 tcgtaacctg caatttatgc cgaaggtttt attgctggat gaaataacca gtgcgctgga
     7621 tgaaagtaat aaacataacg tcaatgagat gatccatcgt tatgtgcgtg agcaaaatat
     7681 tgccgtgctg tgggtgacac acgataaaga cgaaattaat catgcggata aagtgattac
     7741 actgcaaccg catgccggag aaatgcagga agcacgctat gaactcgcat aatattacta
     7801 acgaatcatt agcactggca ttaatgctgg tggtggtggc aatcttaatt agccataaag
     7861 aaaaactggc gctggagaaa gatattctct ggagcgtcgg gcgagcgata attcagctga
     7921 ttattgtcgg ctatgtgctg aagtatattt tcagcgtgga tgatgccagc ctgacattat
     7981 tgatggtgtt atttatctgc tttaatgcgg cgtggaacgc gcaaaaacgc agtaaatata
     8041 ttgctaaagc ttttatctca tcgtttattg ccattacggt cggggcggga attaccctgg
     8101 cggtgctgat tctctcaggg tcgattgaat ttatcccgat gcaggtgatc cctatcgccg
     8161 ggatgattgc cggtaacgcc atggtagcgg tggggttgtg ttacaacaat ttagggcaac
     8221 gggtcattag cgaacagcaa cagattcagg agaaactgag tcttggcgcg acgccgaagc
     8281 aggcttcagc gatattgatt cgcgacagta ttcgcgcggc tttaattccg acggtcgatt
     8341 cagcaaaaac ggttggctta gtgagtttac caggaatgat gtccgggctg atatttgccg
     8401 ggattgatcc ggtgaaggcg attaaatatc agattatggt gacctttatg ctgctctcaa
     8461 ccgccagctt gtcgaccatt attgcctgct atttaaccta tcgtaagttt tataattcgc
     8521 gccaccagtt ggtggtgacg caattgaaga agaaatgatg cgatgccgga tgcgcagcat
     8581 cgcatccggc gttgtggttc atgtgccgat caacggaata tcaatacaac aatgcataca
     8641 gctggcggcg atacttcgac gccagtgcat cacccgtacc cagcgcagcg aggatctcct
     8701 ggaacgtttt acgcgtctga ccgtctgcgg cggtgagatc tttacgcaga tgcccgaaca
     8761 gcaactccag cgcctcttca ttgcgcccaa cctgatgcag ttgcagcgcc agttgcgtcg
     8821 ccagtgcggc atcttctgga ttctccgcca cctgctgttg caactgttga atttccggcg
     8881 tatcagccgc ctgcttcagc agttcgattt gcgccaccag cccctggtag cgggtgtcct
     8941 gatcctgcaa cggaatggtt ttcagcaccg cttccgcatc ttcagaacgg ttcagcgcaa
     9001 tcagcgtttc tgccagcagc aggccgatct ccccgttctg attcgacaac tgccaggcgt
     9061 ctttcagcaa tggcagggca tcggtgtaat tgccttcctg catcagttgc atcgcctgct
     9121 gcgctttcag ctcttcttcg cgcggcagca ctttatccag cagggcgcgg atcgcctctt
     9181 ccggttgcgg cccctggaag ccatctaccg gttgcccgtt ctggaacaga tacacggtcg
     9241 gaatcgcgcg cagaccaaac tgggcggcaa tcatctgctc cgcgtcgcag tccagcttcg
     9301 ccagaataaa ttgcccgttg tactgcgccg caaggctttc caggatcggg gttaactgta
     9361 aacagtgctg gctacgttca gaccaaaaat agaacagcac cggagtggtc atcgactgtt
     9421 caagaacctg ttgcaggtta gattcgttaa tgttgacaat attttctacg gacatggagt
     9481 cgctctctgt tgtcgatatt ttctttgaca tgggggctta agcgcgcgct tcaactcacc
     9541 cctgcaatat tttgtccatc acgcgccccg gcagcaggcg cttgagcacc attactgccc
     9601 aggtcaccag cgttaccgga tagcgcatct tcggcttctc gctaataaaa gcatggcgta
     9661 ctttgtccac caccgcttcc ggtcccaacg taaagcgggc ggcgatgccg ggattttcga
     9721 ctggtttatc actttgcgtc tggttgacgt tgtcggtgaa gcgagtacga atgggaccgg
     9781 gttcgatcag gctgacttta attccgctgt ggcgcagctc catgcgcagt gcgtctgacc
     9841 acgcctccag cgcatattta ctggccgcgt aagcgccacg acccggcgtg gagattaatc
     9901 ccatcaccga tgatgtcatc acaatacgcc cttcaccgtg cggtaacatc gcgggtaaca
     9961 ggcgcatggt gagctggtgt gcgccgaaaa agttggcgga aaactgctgt tccatctgcg
    10021 cacggctgat ggtggaaagg gggccataca tgccgaatcc ggcattgtta aagatcccat
    10081 acagacaatt atcggtcagg gcgatcactt cgtcggctgc gcgatcaaca ctttctggcg
    10141 aatccagatc gatcaacacg ccggtaaatc ccatgctgtt catacgctca acatcatccg
    10201 gtttccggca acctgccagc acatgaaaac cctggcgttt taattcgagc gcgctttcca
    10261 ggccaattcc actggaacat ccggtaatta agaccgattt ttgcataact ttacctgtca
    10321 ggatctccgt tgctttatga gtcatgattt actaaaggct gcaactgctt cgccatccag
    10381 tcggcaataa acggctgggc gtcgcggttg ggatgaatac cgtcatcctg catccattgt
    10441 ggcttgaggt agacctcttc cataaaaaag ggcagcagcg gaacatcaaa ctctttggcg
    10501 agtttggggt aaatggcgct aaaggcttca ttataacggc gaccatagtt tgcaggcaga
    10561 cgtatttgca ttaacaatgg ttcagcgttg gcggctttga catcctgcaa aatctggcgc
    10621 agcgtttgct cggtttgctg tggctgaaaa ccacgcaaac cgtcattgcc gcccagttca
    10681 accagcaccc aacgcggctg atgctgtttc agcagagccg gaaggcgcgc cagtccttgt
    10741 tgcgaggtgt cgccgctgat gctggcatta actaccgacg ttttactctg ccacttatca
    10801 ttcaacaagg caggccaggc cgcgctggca gacattcgat acccggcgct caggctatca
    10861 cccagaatca ataacgtgtc cgctgcggcg gcacggaagg ttaacaggac caggaacagg
    10921 aagggcaaat gccagcggaa aacattgttg aagttcatca tcttaagaag tccgtcggtc
    10981 agggggagca tgaactctcc atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga
    11041 ccatcgcact ggtgggcgag tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg
    11101 ggcttgatga cggcagcagt ggcgaagtga gtctggtggg acaaccgcta cataatatgg
    11161 acgaagaagc gcgggcaaag ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta
    11221 tgttaattcc tacccttaac gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg
    11281 agagtagcgc ggaaagtcgt aacggggcga aagcgttgct cgaacagtta gggctgggta
    11341 aacgtctgga tcatcttccg gcacagcttt ccggcggtga acagcaacga gtggcgctgg
    11401 cacgagcctt taatggtcga cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg
    11461 accgccagac gggcgataaa attgccgacc tgctgttttc cctcaaccgt gaacatggca
    11521 ccacgttgat tatggtgacc cacgacctgc aactggcggc acgctgcgac cgctgcttac
    11581 ggctggtgaa cgggcagttg caggaggaag catgattgca cgttggttct ggcgcgaatg
    11641 gcgttcgccg tcgctattaa ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct
    11701 ggcgctgggc aatatcagcg atcgcatgga gaagggctta agccagcaaa gccgtgagtt
    11761 tatggcgggc gatcgggcgt tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga
    11821 agcgcaaaag cgcggcctga aagtcggcaa gcagctgact ttcgccacaa tgacctttgc
    11881 aggcgacaca ccgcagctgg cgaacgtcaa agcggtggat gatatctacc cgatgtatgg
    11941 cgatctgcaa actaatcccc ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc
    12001 acgcctgatg gcactgctta acctgaaaac gggcgacacc attgacgtgg gcgatgccac
    12061 cttgcggatt gccggagaag tgattcagga accggattcc ggttttaacc ccttccagat
    12121 ggctccgcgt ctgatgatga atctggcgga tgtcgataaa accggagccg tgcagccggg
    12181 gagtcgggtc acctggcgtt ataaattcgg cggcaacgag aaccagctcg acggctatga
    12241 gaaatggttg ttacctcagc ttaaacccga acaacgctgg tacggtctgg aacaggacga
    12301 aggcgcgctg gggcgatcga tggaacgctc gcaacagttc ctgctgcttt cggcgcttct
    12361 gaccttgctg ctggcagtgg cagcggtggc ggtagcgatg aatcattact gtcgcagtcg
    12421 ctacgatctg gtggcgatcc tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct
    12481 aatcgtcggt cagtggttga tggtgctgac gctttcagcc gttaccggtg gggccatagg
    12541 tctgttgttc gaaaacgtgt tgatggtgct gctcaagccc gttctgcctg ctgcactacc
    12601 gccagccagc ctctggccgt ggctgtgggc gcttggcacc atgacggtca tctcgctgct
    12661 ggtggggcta cgaccatatc gcttgttgct ggcaacgcag cctttacgcg tattacgtaa
    12721 tgatgtggta gcgaacgtct ggccgctgaa gttttatctg ccgattgtca gtgtggtggt
    12781 tgtgctgctg ctcgccggat taatgggtgg cagcatgctg ctttgggcgg tgctggcggg
    12841 cgcggtagta ctggctttgc tgtgcggtgt gctgggctgg atgctgctga atgtacttcg
    12901 ccgcatgacg ctgaaatcgc tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc
    12961 gtggtcaacg ttaagtcagc tttcggcatt ttcgctctcc tttatgctgc tggcgctgtt
    13021 gctggtattg cgtggcgatc tgctcgaccg ctggcagcag cagctaccgc cagaaagccc
    13081 gaactacttt ttaattaaca tcgccacaga acaggtagca ccgctaaaag cgttcctcgc
    13141 ggaacatcat atagtcccgg aatcatttta tccggtagtg cgggcgcggc tgacggcgat
    13201 taacgataag ccgacagaag gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac
    13261 ctggcaaaat acgcggcccg atcataatcc gattgtcgcc ggtaactggc cgccaaaagc
    13321 cgatgaagtg tcgatggaag aggggctggc gaaacgctta aacgttgccc tcggtgatac
    13381 cgtgactttt atgggcgata cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt
    13441 ggactgggaa agtctgcggc ctaatttcta ttttattttc ccggaagggg cattagacgg
    13501 gcaaccgcag agctggctta ccagtttccg ctgggagaat ggcaacggca tgttgacgca
    13561 actcaaccgc cagttcccga ccattagcct gttagatatt ggcgcgattt taaaacaggt
    13621 cggtcaggtg ctggagcagg taagtcgggc gctggaggtg atggtggtgc tggtcaccgc
    13681 ctgcggtatg ttgctgttgc tggcacaggt gcaggtggga atgcgccagc gtcatcagga
    13741 gctggtggta tggcgcacac tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg
    13801 tgagttcgcc atgcttgggt ttgtttccgg cctggtggcc gcaattggtg cggaaacggc
    13861 actggcagtg ttgcaggcga aagtgtttga tttcccgtgg gagccagact ggcgattgtg
    13921 gattgttctg ccgtgcagcg gtgcgctgct gctgtcgctt ttcggcggct ggctgggcgc
    13981 gagactggtt aagggtaagg cgctgttcag gcagtttgcg gggtgatgac aggtgttaga
    14041 ttgcctgatg cgctacgctt atcaggccta cgaagagcgt gcaatatatt gattttgcaa
    14101 gattgtgtag gccggataa
//
LOCUS       DACAPB010000121        14033 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.121, whole genome shotgun sequence.
ACCESSION   DACAPB010000121 DACAPB010000000
VERSION     DACAPB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.121"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..714
                     /locus_tag="IT904_003165"
     CDS             <1..714
                     /locus_tag="IT904_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP3192908.1"
                     /translation="MTHKLVSSDYAILMVILSNTLSSIMGVYFIYKVMNENNANDDIP
                     RLVFIKYYIKQYAMQVFGIVTNNMDKYLIGNFTGISALGLYSTAAAFNTLPLKFYNVL
                     SDYLFSGYVNKKNNEKIVLQVAIFGGVIGCLCAFAFSELLIKIAFGERYLSSHVYLPY
                     IIINMVISGIAWVLTQKALISGSQVLIIIRQLIGLIAFAAIFFFLQPYGLWGAIIALM
                     TGSIIRLIISILFFIKIKI"
     gene            725..1879
                     /locus_tag="IT904_003166"
     CDS             725..1879
                     /locus_tag="IT904_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP3192909.1"
                     /translation="MKIMMFNTLYYPFKVGGAEVSVKLLAESLVKKGHEVQVVSITNE
                     NQRKVTLIEGVTSVCIPNTNIYWPFVSEKRNPFQKLFFHIKDNFNFSIQKAISAEIQS
                     FKPDIVHTNNLFGLSTLTWLTCARNSVRVVHTTRDYYLLHINASLYKNGKNINPCGIQ
                     VKLLSFFKAIMSKRVAAFVGISEYMMQLHQKNGFAKHGIHTTIYNTVTKQEVKRKSDE
                     QVIIGFFGRLTVEKGFDTFCKIAHQHQNNQKKIKFIAAGPTDSLTEELKAMAGDAGVD
                     LTGFMTLSDFLSTTDLIILPTKWNEPFGRSVVELALAGKPVFTNLTGGTKEIAALFPN
                     IFPLEDFSVSKIYENQQFKIGEGVVEKFSSERICNQYESLYKRVLNNGKN"
     gene            1836..3128
                     /locus_tag="IT904_003167"
     CDS             1836..3128
                     /locus_tag="IT904_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HAP3192910.1"
                     /translation="MNHSTREFLIMEKTKLSLVDRLLALCFVISIIFTARTFELLELP
                     LYYFIEIGVLVGILVLLIVIYVMGRSTNVSLDKKVLFTAGLTLFLNVFSPIMANINFG
                     QSIILGILAFRIPIMAAVIYLLYYGIFKYKVKVDNERFFEYVISWLFIISLLITLVWI
                     YLTYTLNAEYYYFSSGSLLVDTRSDGSYRFRFDIFFPFMLLCCSWYKSSKNKSRYPIV
                     YWFTLGLSIIYLIFYFQGRVFIGALALSFFLCYIRTYVYSTRYVILYLLVFISVLLSI
                     VVYFTYGELIELYQNMLRSLSLNDSSSLVRLRILRVMQNNFIDNFLLGNGYLSNQADI
                     NMGDTKFSPLDIGVLGVIYTYGFILFLITFSWFSKLWLDSFNKINAKLYTFCFSVLYL
                     NMLFTGGFFFRPYQIMLLLIFHMLFNRNKKDLKKGSVY"
     gene            3128..4216
                     /locus_tag="IT904_003168"
     CDS             3128..4216
                     /locus_tag="IT904_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP3192911.1"
                     /translation="MKALVFLGLRHTILADIWQKEYQEGKRNDSMLYGYGNAVTPGIQ
                     VDYCRFNKFERLFFKDNIIGKLYLYCLKLPLLLLKYDIVWTHVDRDALFIAKLRRLPI
                     INNFFAKHIANFVWLIDHSQNYSDRQLKKTQKLLKTIDKILYLSTAEKDEFINKYQCE
                     QSQLLYTRYGINFSAYDYNALSVKPIGFDKEDFILSVGTDQHRDLELLDVIAKNNPDK
                     QFVICSGNPEHHKRNFSRDNVTIIRASYNEMVYLYHACKFVVIPLKFNFHASGITTLL
                     EAAAARKAVLLNYTPGLEDYGQHDNTCLFASLGDIESFDRNLNKLWDSEKLRNRLAEN
                     AYKYLHSDFNTYKYSQIYIELSKNILGI"
     gene            4228..5397
                     /locus_tag="IT904_003169"
     CDS             4228..5397
                     /locus_tag="IT904_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP3192912.1"
                     /translation="MNILFLTTILPIKAKSGGEIVTKQIITGLISNGADVDVVGYVRP
                     DETNLSLPTNYNVAEARVIETQGHKKETLKWLLSSLVKKVPFSSEKYVGAKFIEIVNA
                     KLRQKQYDYIFIDHTQMLWICNYFSNDQKLILISHNVESQLYDDLANDSSNWLKRNIL
                     RLESSKLEKLEQSSINKISQIWSLSKNDTAYYSKLNNSQYNSPTCITFDALPLLEPDL
                     LPLPEAHYDVSLLGTWSWDANMKGLRWFFDNVYPLLPVEITIQVAGKGADWLADKYKN
                     VKYKGFVEDAQEFLSKSKVIAIPSISGAGVQIKTLDAITIGRPIVASEFALRGLEDIP
                     SYVYSSRQASEMANQIMTNLERKVSQNDLLVLKHECNEWYSSKINNFKEVILGSL"
     gene            5407..6174
                     /locus_tag="IT904_003170"
     CDS             5407..6174
                     /locus_tag="IT904_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP3192913.1"
                     /translation="MEMINLKKFDTITINDVSMVNFLDIIHNNFNRKDGLKIIVTPNI
                     DHLQRLRNSDDPSFCKAYEISDYRLCDSRIVQKLSSFNEQNILNVVPGSDLTRELLRL
                     DWVRRAKVTIIGTTEKQVEQLKKNFQLHNIEHFNPSMGFAKRNNEVEACVDFIINSNP
                     DFVFLAVGSPSQEHLAFSLYQRINSVNPNSFAAQLFCIGASLDFLTGKTKRAPIWMQK
                     AHLEWLHRALSQPRRLVPRYYRNLKYVLWFLLFRNRA"
     gene            6195..7943
                     /locus_tag="IT904_003171"
     CDS             6195..7943
                     /locus_tag="IT904_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase superfamily protein"
                     /protein_id="HAP3192914.1"
                     /translation="MKRRSLLFKSFIATFAYIGLSNKSQSADVTQIPANKEKNNLRPP
                     VSVDNSNFQIVSALSKPTGLALIGQCESINELRTIIPENHGQRISVKGYYPSSNEGGG
                     VFYYDENDKTSKDNDGTIIISKGRRWKRENTYLMEPEFFGAKGDGSTDDTTALQNALD
                     ASINKVLILAPKKYRARNLFIKGPLIIKGAGRRIDAALIPLDGVSGNFITISCPESPT
                     FYDLTISARNVNQSSPLNGLYIAKNDTKQYSPSILMYNCNINSFLGHCLLSEYGRHMG
                     VLDNCQFEYSHDDCIVINGVDWNISHCAIGYTKNGNGVVLGQPTNRISNSDIYFNRRN
                     GIVLKNNAINGYLASNVINSNGEHGIVILSKNPSKQGHLIVNNFFFNNSKSQSGQYSN
                     IFLSKNIHGVGITGNSHFTYSEQSERPKHLLELDDGASGSLIGDSFDKSSYMISPVNG
                     SKSTIINNEYTTLGSETEFKKIVSNDNSKSAFIVENTEARRKILEITNRGEFVFYDHN
                     NSPLTISASKIVDNSLELNCSLAIGSNEADHDKIYFLKLGDFYIWDDSAGNIRIHTEQ
                     PHSKTQGNILYQTKSH"
     gene            7951..8904
                     /locus_tag="IT904_003172"
     CDS             7951..8904
                     /locus_tag="IT904_003172"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP3192915.1"
                     /translation="MQNANRNEWVDYAKGFGIVLVVYEHISRGLYNSGIISDTKWFHF
                     IDSLIYSFHMPLFFFLSGLFLMKSLQVRGLPYFIFNKIDTILYPYLIWSVSLFLNFIL
                     ISLILSLKEKLKINTTIVIAFSFLFAIFIYLAFSNILDTNPLNYIVHNSVFILSGIVF
                     SYYRLDIKIQNNIVFFATGFIVPFIFQAIYIYKNVMGYKFSVLALAFSLIGTGVIVAL
                     CIAFSKLDKAFIKNLLCFLGRYSMPIYLMHVLVGSGVRIFMSKILGVYNVGIHLFLGI
                     FLGILMPILSYKFFKKMNINLFSLSGVFSLENLFLRKRSSI"
     gene            9123..10520
                     /gene="wcaJ"
                     /locus_tag="IT904_003173"
     CDS             9123..10520
                     /gene="wcaJ"
                     /locus_tag="IT904_003173"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAP3192916.1"
                     /translation="MIFSHHRTRSNTNASLLSMVQRFSDISIIFIGIYVACLLNGESV
                     QYKHILIFLTVLVIFQMIGGITDFYRSWRGVDLSAEIILIFKNWTLSLLLSLGGFSLL
                     ADLNIEYIIFLQWFFIVCFGFFVCRLSIRIILGMLRKIGYNTRKVAVAGSLPAGLNLL
                     RTFQNAPWLGFVVKGIYDDIKIDSYEDVPYGGTLSELVEDARSGKIDRIYIAMSMKDE
                     TRLRDIISQLTDTTCSVLLIPDVFTFNILQSRTEEINGVPVVPLFDTPLNGINMVFKR
                     IEDVVVSSIILLLISPVLCTIAVIVKVTSPGPVIFRQIRYGMDGKPIRVWKFRSMTVM
                     ENDTKVIQATKNDVRVTKVGKFLRRTSLDELPQFFNVLFGQMSVVGPRPHAVSHNEQY
                     RSLIQGYMLRHKVKPGITGLAQINGWRGETDTLEKMEKRIEYDLMYIRSWSVWLDLKI
                     IFLTVFKGFINKSAY"
     gene            10687..12093
                     /gene="gndA"
                     /locus_tag="IT904_003174"
     CDS             10687..12093
                     /gene="gndA"
                     /locus_tag="IT904_003174"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP3192917.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGGLALSNEELAQT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAGFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAQNAGIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAIAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLE"
     gene            12321..13736
                     /locus_tag="IT904_003175"
     CDS             12321..13736
                     /locus_tag="IT904_003175"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HAP3192918.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRHG
                     DDPLMLVLAADHIINNQPVFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHTPYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDIL
                     EACQAAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLTENQSTFIPIGAEHSLENPARIPLEVLEI
                     QSGSYLGEDDIIRIKDQYGRC"
     gene            13759..>14033
                     /locus_tag="IT904_003176"
     CDS             13759..>14033
                     /locus_tag="IT904_003176"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP3192919.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGG"
BASE COUNT         4236 a         2472 c         2805 g         4520 t
ORIGIN      
        1 atgactcaca agttagtaag ttcagattac gcaattttga tggttatttt atctaatacg
       61 ctatcaagta tcatgggagt gtatttcata tataaagtta tgaatgaaaa taacgcaaat
      121 gatgacatac cccgtttagt gtttataaaa tattatatta aacagtatgc aatgcaagtt
      181 tttggcattg tgacgaataa tatggataaa tatttaatag gtaactttac aggtatttca
      241 gcgctaggtt tatactcaac agcggcagct tttaacacat tacccctcaa attttataat
      301 gtcttatcgg attatttatt ctcaggttat gttaataaaa aaaataatga aaaaatcgtt
      361 ttacaagttg ctatatttgg aggggttatt ggttgtttat gtgcatttgc attttctgaa
      421 ctactaataa aaatagcgtt cggtgaaaga tatttatcat cgcatgtata tcttccttat
      481 attatcatca atatggtaat tagtgggatt gcatgggtgt taacacaaaa agccttaata
      541 tcaggtagcc aggtattaat cataataagg caactaattg gattgattgc atttgcagct
      601 attttcttct ttttgcaacc ttatgggttg tggggagcga tcattgcatt aatgactggt
      661 agtattatta gactgataat atcaattctt ttctttataa aaattaaaat ataaggataa
      721 tcaaatgaaa attatgatgt ttaatacatt atactatcca tttaaggtcg gaggtgccga
      781 agtatcggta aaactcttgg ctgagtcgtt agtcaagaag ggacatgaag tacaagttgt
      841 atcaattaca aatgaaaatc aaagaaaagt aacattaatt gaaggggtaa catctgtttg
      901 cataccaaat accaatatat attggccctt tgtaagtgaa aaaagaaatc cttttcaaaa
      961 actatttttt catattaaag ataattttaa tttcagtata caaaaagcta tttcagcaga
     1021 gatacaaagt ttcaagcctg atatagtaca tactaataat ctctttggct tatcaacact
     1081 aacttggctt acatgtgcaa gaaattcagt tagagttgta catacaacca gggattatta
     1141 tctgttgcat attaacgcat ctttatacaa gaatgggaaa aatatcaatc catgtggtat
     1201 acaagtaaag ctcttatctt tttttaaagc gataatgtcg aaaagagtgg ctgcatttgt
     1261 tggtataagt gaatatatga tgcagcttca tcaaaaaaat ggttttgcaa agcatggaat
     1321 ccatacaact atatataata ctgttactaa acaggaagtt aaaagaaaat ctgatgaaca
     1381 agttataatt ggcttttttg ggaggctaac agtagaaaaa ggctttgata ctttttgtaa
     1441 aatagcacac cagcatcaaa ataatcaaaa aaaaataaag tttattgctg ctggtcccac
     1501 agactcgtta accgaagaat taaaagcaat ggctggtgac gctggagtgg atttaacggg
     1561 ctttatgact cttagcgatt ttttatccac tactgatttg attattcttc ctactaaatg
     1621 gaatgagcct tttggtagga gtgttgtgga attggcatta gctggtaagc ctgtatttac
     1681 aaatttgaca ggcggaacga aagaaatcgc tgcactattt ccaaatattt tccctctcga
     1741 ggatttttct gtctcaaaaa tatatgagaa tcagcaattt aaaataggtg aaggtgtggt
     1801 tgaaaaattc tctagtgaaa gaatttgtaa tcaatatgaa tcactctaca agagagttct
     1861 taataatgga aaaaactaaa ctttctctgg tagatcgact tttagccctt tgctttgtaa
     1921 tatcgataat tttcactgcg cgtacctttg aactactaga gcttccatta tattatttta
     1981 ttgaaatagg tgtcctcgtt gggatcttag ttcttttaat agttatttat gtgatggggc
     2041 gatcaactaa tgtttctctc gataaaaaag tgctatttac ggctgggttg actctatttt
     2101 taaatgtgtt ttctccaatt atggctaata tcaattttgg acaaagcatt atattaggta
     2161 tattagcttt cagaatacca attatggctg ctgtaatata tttattatat tatggtatat
     2221 ttaaatataa agtaaaagta gataatgagc ggttttttga atatgtaatt tcgtggttat
     2281 ttataatatc tttattgata acattagtgt ggatatatct tacttatacc ctaaatgcag
     2341 aatattacta tttttcaagt ggtagtttat tagtcgatac gcgtagtgat ggatcatatc
     2401 gttttagatt cgatatattc tttccattta tgttgttatg ttgctcgtgg tataaatcat
     2461 caaaaaataa atcaagatat ccaattgttt attggtttac tttaggattg tctataattt
     2521 atttgatttt ttattttcag ggccgagttt ttataggtgc cctagcatta tcttttttct
     2581 tatgctatat aagaacgtat gtatacagca caaggtacgt aatcctttat cttttagtct
     2641 ttatttcagt tttattatca attgtggtgt atttcactta cggtgaactc atagagttat
     2701 accagaacat gcttcggtcg ctttcgctca atgacagttc atctttagta agactaagaa
     2761 tattaagggt tatgcaaaac aattttatag ataactttct ccttgggaac ggatatctca
     2821 gtaaccaagc tgatataaat atgggggata cgaaattcag tcctctagat ataggtgttt
     2881 taggcgtgat ttatacgtat ggctttattc tatttttaat aacatttagt tggtttagta
     2941 agctatggtt agattcattt aataaaataa atgcaaagct atatacattt tgctttagtg
     3001 tattatattt aaatatgcta tttacaggtg gtttcttttt tagaccatat caaatcatgt
     3061 tgttacttat tttccacatg ctttttaacc gaaataaaaa agatttgaaa aaagggagtg
     3121 tttattaatg aaagcgctgg ttttcctagg attacgacac acaatattag ctgatatatg
     3181 gcaaaaagag taccaggaag gaaaaagaaa tgatagtatg ctttacggat atggaaatgc
     3241 agtaacgccc ggcatacagg ttgattattg tagatttaat aaatttgaac gacttttttt
     3301 taaagataat ataattggca aattatattt gtactgcctc aagctcccat tgctgctttt
     3361 gaaatatgat attgtttgga cgcatgttga tagagatgcg ctttttattg ctaagttaag
     3421 aagattacct atcatcaata acttttttgc aaagcatatc gctaattttg tttggttaat
     3481 agaccattcg caaaattatt cagatagaca gcttaagaaa acacaaaaat tattaaaaac
     3541 tattgataag atattgtatc tatctacagc tgaaaaagat gagtttataa ataaatatca
     3601 atgtgaacaa agtcaactac tctatacaag atatggtatt aacttttcag cgtatgacta
     3661 taacgccctg tcagttaaac ctattggatt cgataaagaa gattttattt tgtccgttgg
     3721 aacagatcag catcgagatc tggaattatt agatgttatt gcaaaaaaca atccagataa
     3781 acaatttgta atttgttctg gaaaccctga gcatcataaa cgaaattttt ctagggataa
     3841 tgttactatt atcagagctt catataacga aatggtatat ttataccatg catgtaaatt
     3901 tgtagtgatc cctttaaagt ttaattttca tgctagtggc atcacaactc tattagaagc
     3961 ggctgcggct agaaaagcgg ttttattaaa ttacactcca ggtcttgaag attatggcca
     4021 acatgataat acctgccttt ttgcttcgtt aggagacatt gaatcgtttg ataggaacct
     4081 taacaaacta tgggatagtg aaaagttaag aaaccgtctt gctgaaaatg catataaata
     4141 tttacattct gatttcaaca catataaata ctcacaaata tatattgaac tgagtaaaaa
     4201 tatacttggc atataaagta gaggcctatg aatatattat tcttaacaac aatcttacct
     4261 ataaaagcaa agtcaggagg ggagattgtt acaaagcaga taataaccgg attgatttca
     4321 aacggtgccg atgttgatgt ggtcggctac gttaggccag atgaaacaaa tttgagcctt
     4381 ccaacaaatt ataatgtcgc tgaagcaaga gtcattgaaa cacagggaca taaaaaggaa
     4441 actttgaagt ggcttttgag ttccttagtt aaaaaggtac cattttcatc tgaaaaatat
     4501 gttggcgcta agttcattga aatagttaat gctaagctca gacaaaagca atatgattat
     4561 atatttattg atcatactca gatgctatgg atatgtaatt atttttcaaa tgatcaaaaa
     4621 ttgattttga taagtcataa tgttgagagt cagctttatg atgatcttgc aaatgactct
     4681 tcaaattggt tgaaacgtaa tattctacgt ttggaatcat cgaaattaga aaaacttgaa
     4741 caatcgtcta taaataaaat tagccaaata tggtcattga gtaaaaatga tacagcgtat
     4801 tattcaaagc taaataactc acaatataat agtcctactt gcataacttt tgatgcattg
     4861 ccattattag aaccagattt attacctcta cctgaagcac attatgatgt atcactcctt
     4921 ggtacttggt catgggatgc gaatatgaaa gggcttcggt ggttttttga taatgtatat
     4981 cctttgttac cagtggaaat tacaattcaa gttgctggaa aaggtgctga ttggctagcg
     5041 gataaatata aaaatgtcaa atataagggg tttgtcgagg atgcccaaga attcttgtcg
     5101 aaatctaagg ttattgcaat accatctatt tctggcgctg gagtccaaat aaaaacgtta
     5161 gatgcaatta ccatcgggcg ccctattgtt gcatccgagt ttgcattacg cggtttagaa
     5221 gatatacctt cttatgttta tagttcacgt caagcttctg aaatggccaa ccagattatg
     5281 accaatcttg aaagaaaagt aagtcaaaat gatcttttag ttctaaagca tgaatgcaat
     5341 gaatggtatt caagtaaaat taataacttc aaagaagtaa tattaggaag tttataggtt
     5401 ggagttatgg aaatgattaa cttaaaaaag tttgacacca ttacaattaa tgatgtatca
     5461 atggttaatt ttcttgatat tattcataat aatttcaata gaaaagatgg attaaaaatc
     5521 attgttactc caaatattga tcatttacaa cgcttgcgaa attcagatga tccttcattt
     5581 tgtaaagcgt acgaaatatc agattataga ttatgtgata gtcgtattgt acaaaagctg
     5641 tcctctttta atgaacaaaa tattcttaat gtagttccag gcagcgattt aactagagag
     5701 ttacttaggc ttgactgggt gagaagggca aaagtaacta ttataggaac aacagagaaa
     5761 caagtagaac aattaaaaaa aaattttcaa ttacacaata ttgaacattt taatccttca
     5821 atgggatttg cgaaaagaaa taatgaagtt gaagcttgtg ttgattttat tattaacagt
     5881 aatcctgact ttgtgttctt ggctgtaggg tctccatcgc aggaacacct cgcattttca
     5941 ctctaccaaa gaataaatag cgtcaatcca aattcttttg cggctcaact attttgtatt
     6001 ggtgcatctt tagactttct tacagggaaa actaaaaggg ctccgatatg gatgcagaaa
     6061 gctcacttag agtggttaca tagagcgtta agtcaaccga ggagacttgt cccgagatat
     6121 taccgaaatt taaagtatgt attatggttt ttattgttta gaaatagagc ctaagtttgt
     6181 gaccggagag caaattgaaa agaagaagct tgttatttaa gtcttttatt gcaacctttg
     6241 catatatagg attgtctaat aaaagtcaat ctgctgatgt cactcaaatt cctgctaata
     6301 aggagaaaaa taatctaaga cctccagttt ctgttgacaa ttcgaatttc caaatcgtat
     6361 cagcattatc aaaacctaca ggtttagctt taatcggaca atgtgagtcg attaacgaat
     6421 tgagaacaat tattcctgaa aatcatggcc aacgaattag tgtaaaaggt tattatccct
     6481 cttctaatga aggcggaggt gtgttttact atgacgagaa tgataaaacc agcaaagata
     6541 atgacggaac tataattatt tcaaagggaa ggcggtggaa gcgtgaaaat acatacctga
     6601 tggaacctga gttttttgga gcaaaagggg atgggagcac cgatgataca actgctttac
     6661 aaaatgcact tgatgcttcg attaacaaag ttcttatttt ggcgcccaaa aaataccgtg
     6721 ctcgaaatct ctttattaaa ggtccactta ttattaaagg tgccggaaga cgaatcgatg
     6781 cggccttgat tccacttgac ggtgtttctg gtaactttat tactatttct tgccctgaat
     6841 cacctacttt ctatgacttg accatctcag ccagaaatgt aaatcagagc tctccattga
     6901 atgggttata tatagcgaaa aacgatacta aacaatattc accctctatt ttaatgtata
     6961 attgtaacat aaactctttt ttaggacatt gtcttctatc cgaatatggt cgtcatatgg
     7021 gagtacttga taattgtcaa tttgagtaca gccatgatga ttgcatcgta ataaacggtg
     7081 tcgattggaa tatatctcat tgtgctattg ggtatactaa aaatggaaat ggcgtagtcc
     7141 ttgggcaacc tacgaatagg atttctaaca gcgatatata tttcaatcgc cgtaacggta
     7201 ttgttttaaa aaataatgca attaacggtt atcttgcaag taatgttatt aatagcaatg
     7261 gtgagcatgg aattgttatt ttaagtaaaa atcctagtaa acaaggccat cttatcgtaa
     7321 acaatttttt ctttaataac tccaagtcac aatcaggaca gtattctaat atttttctgt
     7381 caaaaaatat tcatggtgtt ggaattactg ggaattcgca ctttacttat tctgaacaaa
     7441 gtgaacgacc gaaacacctt cttgagttag atgatggagc ttccggttct ttaattggag
     7501 atagtttcga taaaagtagt tatatgatat cccccgtaaa tggttcaaaa tcaactataa
     7561 ttaataatga atatactaca ttgggttcag aaactgaatt caagaaaatt gttagtaatg
     7621 ataactccaa gtccgctttt atagtcgaga acactgaagc tagacgcaaa atattagaaa
     7681 taacaaatag gggcgagttt gttttttatg atcacaataa ttctccttta actatctctg
     7741 catctaaaat tgtagataat tcattagaat taaactgtag cttggcaata gggtcaaatg
     7801 aagctgatca tgataaaatt tattttttga agcttggtga tttttatatt tgggatgatt
     7861 cagcaggaaa tataagaatt catactgaac agccgcattc aaaaactcaa ggaaatatat
     7921 tatatcaaac taaatcacat tgaggtaatt atgcagaatg ctaacaggaa tgaatgggtt
     7981 gattacgcca aaggttttgg gatagtactt gtagtctatg aacatatttc aagaggttta
     8041 tataattccg gaataataag cgatactaaa tggtttcatt ttatcgattc tttaatatat
     8101 tcctttcata tgccgttgtt cttcttcctt tctgggcttt tcttaatgaa aagtctgcaa
     8161 gttcgcggtc ttccatactt tatttttaat aaaatagata ctatacttta tccttatctt
     8221 atttggagtg tttctctttt tttaaatttt atattgatct ctttaatatt gagtttaaaa
     8281 gaaaaactga aaataaatac aactattgtt attgcttttt cttttctttt tgcaattttt
     8341 atttatctgg ctttttcaaa tatccttgat acaaatcccc ttaattatat tgtgcacaac
     8401 tctgtgttta tactatcggg aattgttttc tcttactata gattagatat aaaaatacaa
     8461 aacaatattg ttttttttgc gactggtttt atagtgcctt ttatttttca agctatatat
     8521 atatacaaaa atgtaatggg ttacaaattt agtgttttag cgcttgcttt ttccttaatt
     8581 ggaacaggtg ttattgtagc actttgtatt gctttttcta agcttgataa agctttcatt
     8641 aaaaacttac tatgtttcct tggccgttat tctatgccaa tttatcttat gcatgttttg
     8701 gtgggttctg gtgttcggat ttttatgtca aaaattttgg gtgtttacaa tgtgggcatc
     8761 catctctttt taggaatctt tttgggtatt ttaatgccta tattatctta taaatttttt
     8821 aaaaaaatga atattaacct tttctctttg agtggtgttt tttctctgga gaatctgttc
     8881 ttgagaaaaa gaagtagcat ttgatgttct aggtttaatt ttctttccga aaagctacgc
     8941 tctatgccat gtgttaatat ttttcttcac ttcttttttg ttactaaaac agtataacta
     9001 attttatttt tgcgggatcg aagatgtttt tatgtctgaa atatgttttt gtgaatattt
     9061 ataaatatct taatgaatta gtgattctaa acagttcaga atttacaaaa gagtaaataa
     9121 ctatgatatt ctcacatcat cgtactagat caaatactaa tgcatcgttg ttatctatgg
     9181 tacagcgatt ttctgatatt agcattattt ttatcggcat atatgttgct tgtttactca
     9241 atggggaatc agttcagtat aaacacattc ttatatttct cactgtgctt gtcatatttc
     9301 aaatgatagg tggtataacc gatttctatc gttcgtggcg tggagttgat ttatctgctg
     9361 aaataatttt aatttttaag aattggacgt tgagtctatt gctgtcgtta ggtggatttt
     9421 ctcttctagc agatttaaat atcgaatata ttatttttct acaatggttt tttatagtat
     9481 gttttggttt ttttgtttgt agactttcga tccgtattat tttgggaatg ttgagaaaaa
     9541 ttggttataa tacgaggaaa gtcgccgtgg caggctcgtt acctgctgga ttgaatcttt
     9601 taagaacttt tcaaaatgct ccttggcttg ggtttgttgt taaaggtatc tatgacgata
     9661 ttaaaattga tagttatgag gatgtcccat acggagggac gctctctgaa cttgttgaag
     9721 acgctcgctc aggaaaaatt gatcggatat atatagcaat gagcatgaaa gacgaaacaa
     9781 gattgaggga tattatttct caattaaccg atactacatg ctctgtgtta cttattcctg
     9841 atgtttttac tttcaatata ttgcaatctc gaactgaaga aatcaatggt gtacctgtag
     9901 ttccgttgtt tgatactcca ctcaatggta ttaatatggt ctttaaacgg attgaggatg
     9961 tcgttgtttc ttcaattatt ttacttctta tatccccagt gttgtgcact attgcagtca
    10021 tagtcaaagt gacttcacct ggacccgtta tattccgcca gatccgttac ggcatggatg
    10081 gtaagcctat aagagtttgg aagtttcgct caatgaccgt aatggaaaat gacactaaag
    10141 ttattcaagc tacaaaaaat gatgttcgtg taaccaaagt tggtaagttt cttcggagaa
    10201 cttctttaga tgaactccct caatttttta atgttttatt cggtcaaatg tctgttgttg
    10261 gacctcgacc gcatgctgtt tctcataacg agcaatatcg atcattaata cagggttata
    10321 tgttacgcca taaagtaaaa cctggaatta ccggcttggc acaaatcaat ggatggagag
    10381 gcgaaaccga tacgcttgaa aaaatggaaa aacgtatcga gtatgatctt atgtacatta
    10441 gaagctggag cgtatggtta gatctcaaaa ttatttttct gactgttttt aaaggtttta
    10501 ttaataaatc ggcatattag aattataatg cgcttaaagt ttgctctgtt catttttaaa
    10561 acataatttt ttttcttgtg ctttataatg aattttcact ataagctctt tgtactttgg
    10621 gctaacatta tcatcacata taagctgcga gtttgtcgca gtgaccacac ctgacaggag
    10681 tatgtaatgt ccaagcaaca gatcggggtt gtcggtatgg cagtgatggg gcgtaacctt
    10741 gcgctgaaca tcgaaagccg tggttatacc gtctccgttt tcaaccgctc ccgtgaaaag
    10801 accgaagaag ttattgccga aaatccaggc aagaagctgg ttccttacta tacagtaaaa
    10861 gagttcgttg aatccctcga aacaccacgt cgtatcctgt tgatggtgaa ggcaggggct
    10921 ggtacagata gcgccatcga ctccctgaag ccttacctcg ataaaggcga catcatcatt
    10981 gatggcggca acaccttctt ccaggacacc atccgtcgta accgtgagct gtctgctgaa
    11041 ggttttaact ttatcggtac cggtgtttct ggtggtgaag agggggctct gaaagggcct
    11101 tccatcatgc ctggtgggca gaaagaagcc tatgagttag ttgctccgat tctgaagcag
    11161 attgctgcgg ttgctgaaga cggtgagcca tgtgtcacct atatcggtgc tgacggtgct
    11221 ggccattatg tgaagatggt tcataacggc atcgagtatg gtgacatgca gctgattgct
    11281 gaagcttatg cgcttttgaa aggcggtctg gcactttcca acgaagagct ggcgcaaacc
    11341 ttcaccgaat ggaacgaagg tgagctaagc agctacctga tcgacattac caaagatatc
    11401 ttcaccaaga aggatgaaga aggtaagtac ctcgttgatg tcattcttga tgaagcagca
    11461 aacaaaggta ccggtaagtg gaccagccag agctcactgg atctcggcga acctctgtct
    11521 ctgataaccg agtccgtatt tgctcgctat atttcttcgc ttaaagacca gcgtgtagcc
    11581 gcgtcgaaag tgctgagtgg tccgcaggcc cagccggccg gtgataaagc tggatttatc
    11641 gaaaaagtgc gtcgcgcgtt gtacctcggt aaaatcgtct cctatgctca gggcttctcc
    11701 cagctgcgtg ctgcttctga tgaatacaac tgggatctta actacggcga gatcgctaag
    11761 attttccgcg ctggctgcat cattcgtgcg cagttcctgc aaaagatcac cgatgcttat
    11821 gcgcaaaacg ctggcattgc taacctcctg ctggcaccat acttcaaaca gatcgctgat
    11881 gactatcagc aagcgctgcg tgatgtggtg gcttatgccg tgcagaatgg tattccggta
    11941 ccgacgttct ctgccgcaat agcttactac gatagttatc gttccgcagt tctgccagct
    12001 aacctgatcc aggctcagcg tgattacttt ggtgcgcata cctataaacg taccgataaa
    12061 gaaggtgtat tccataccga gtggctggaa taaatttatt caaacagtca ggccatttgg
    12121 cctgactgtt tttaatttta tatttaatgt tgcaacctga ccttaagcat ttcctttcta
    12181 aaaatatttc taatatctaa ttttttaatc tttttaagaa cttgccatat ttattatctt
    12241 gtacgcctat attaatctgc ttctggcctc agtaaattac tgggtgttaa ataacagggt
    12301 tttaattaaa ggaaattaat atgttacttc ctgtgataat ggctggcggt accggcagtc
    12361 gtctctggcc gatgtctcgc gagctttacc cgaaacagtt cctccgcctg ttcgggcaga
    12421 actccatgct gcaggaaacc atcacccgac tctcgggcct tgaaatccat gaaccgatgg
    12481 tcatctgtaa cgaagagcac cgcttcctgg tggccgaaca gctgcgccag ctcaacaagc
    12541 tatcgaacaa cattatactt gagccggtcg ggcgcaacac cgccccggcc atcgccctgg
    12601 cggcccttca ggccacccgt cacggcgacg acccgctgat gctggtcctc gccgccgacc
    12661 atatcatcaa taaccagccg gtcttccacg acgccatccg cgtcgccgag cagtatgccg
    12721 atgaaggcca tctggtcacc ttcggtatcg tgccgaacgc cccggaaacc ggctacggct
    12781 acatccagcg cggcgtggcc ctcaccgaca gcgcccacac cccgtaccag gtggcccgct
    12841 tcgtggagaa accggaccgc gagcgcgccg aggcctacct cgcctccggg gaatactact
    12901 ggaacagcgg catgtttatg ttccgcgcca aaaaatacct ttccgagctg gccaaattcc
    12961 gcccggatat cctcgaagcc tgccaggccg cggtcaatgc cgccgataac ggcagcgact
    13021 tcatcagcat cccgcatgac attttctgcg agtgcccgga cgagtccgtg gactacgcgg
    13081 tgatggagaa aaccgccgac gcggtggtgg tcggtctcga tgccgactgg agcgacgtcg
    13141 gctcctggtc tgctctgtgg gaggtcagcc cgaaagacgg gcagggtaac gtcctcagcg
    13201 gcgacgcgtg ggtgcacaac agcgaaaact gctacatcaa cagcgacgag aagctggtgg
    13261 cggccatcgg cgtggagaac ctggtgattg tcagcaccaa ggacgccgtg ctggtgatga
    13321 accgcgagcg ttcccaggac gtgaagaagg cggtcgagtt cctcaagcag aaccagcgca
    13381 gcgagtacaa gcgccaccgc gagatttacc gtccctgggg ccgctgcgac gtggtggtcc
    13441 agaccccgcg cttcaacgtc aaccgcatca ccgtgaaacc gggcggcgcc ttctcgatgc
    13501 agatgcacca ccaccgcgcc gagcactggg tcattctcgc cggcaccggc caggtgaccg
    13561 tcaacggcaa gcagttcctg ctgaccgaga accagtccac ctttattccg attggcgccg
    13621 agcacagcct ggaaaacccg gcccgcattc cgctggaagt gctggagatc cagtcggggt
    13681 cgtacctcgg cgaggacgac attattcgta ttaaagacca gtatggtcgt tgctaatttt
    13741 ttcgggacaa aacgcagaat gacacagtta acatgcttta aggcttatga catccgtggt
    13801 gaactgggcg aggagctgaa cgaggacatc gcctaccgta tcggccgcgc ctacggcgaa
    13861 tttctgaaac ccgggaagat agtggtgggg ggcgatgtgc gcctcaccag cgagtcgctg
    13921 aagctggcgc tggcccgcgg gctgatggac gccggcaccg acgtgctgga tatcggcctg
    13981 agcggcacgg aagagattta cttcgccact ttccacctcg gggtggacgg cgg
//
LOCUS       DACAPB010000122        14027 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.122, whole genome shotgun sequence.
ACCESSION   DACAPB010000122 DACAPB010000000
VERSION     DACAPB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.122"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..261
                     /locus_tag="IT904_003177"
     CDS             <1..261
                     /locus_tag="IT904_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3192920.1"
                     /translation="RVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNN
                     RDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTA"
     gene            complement(537..1427)
                     /gene="galF"
                     /locus_tag="IT904_003178"
     CDS             complement(537..1427)
                     /gene="galF"
                     /locus_tag="IT904_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GalU regulator GalF"
                     /protein_id="HAP3192921.1"
                     /translation="MANLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKDIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQAICPPGVTIMNV
                     RQAQPLGLGHSILCARPVVGDNPFVVVLPDIILDGGTADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPMVAEGQMARIVEFIEKPDEPQTLDSDLMAVGRYVLS
                     ADIWAELERTEPGAWGRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKMGYMQAFVTY
                     GMRNRKEGTKFRESIKKLLA"
     gene            2180..4870
                     /locus_tag="IT904_003179"
     CDS             2180..4870
                     /locus_tag="IT904_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP3192922.1"
                     /translation="MQMQHLMVGYPKYYQTADYALRLSVMADIATMRMKALHFWDKHD
                     ISAASEAFGVSCRTPYWWRQLLNKGGPEGPIPHSKAPLVRRKKHWHPDVLKEIRRLRT
                     ELPNLGKEQIFVRLKPWCAQRHLTCPSVSTIGRMIATAHDKMRMIPVRLSSRGKALFV
                     KKRTTKPRRPKHYRPVKTGELIGMDAIELRMGEMRRYIITMIDEHSDYALALAVPSLN
                     SDIVNHFFSRAARLFPIGISQIISDNGKEFLGSFDKTLQEAAIKHIWTYPYTPKMNAI
                     CDRFNRTLREQFIEFNEILLFEDLALFNQKLAEYLVLYNSKRPHKALALMTPVEYMLK
                     ENKNCNMWWTHTLHFRLILSMIVITHTAVVQVLIHMEWIADPSIWAGLVTLVVIELVL
                     GIDNLVFIAILAEKLPPAQRDRARITGLMLAMVMRLLLLASISWLVTLTQPLFSFRSF
                     TFSARDLIMLFGGFFLLFKATMELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIF
                     SLDSVITAIGIVDHLLVMMAAVVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIG
                     FSLVAEGFGFVIPKGYLYAAIGFSVMIEAFNQLAIFNRRRFLSANQTLRQRTTEAVMR
                     LLSGQKEDAELDAETASMLVDHGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIE
                     HIDLNAPEDEIRQLLERNQHTRLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVL
                     IRQPLVFPETLPLLPALEQFRNARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEV
                     EEIDARHDIQKNADGSWTANGHMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPK
                     PGEEVQVGDYLLKTLQVESHRVQKVQIIPLRKDGEMEYEV"
     gene            complement(5322..7175)
                     /gene="asmA"
                     /locus_tag="IT904_003180"
     CDS             complement(5322..7175)
                     /gene="asmA"
                     /locus_tag="IT904_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAP3192923.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRAYNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPFNETANGENGAAQQGLSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPMLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(7197..7778)
                     /gene="dcd"
                     /locus_tag="IT904_003181"
     CDS             complement(7197..7778)
                     /gene="dcd"
                     /locus_tag="IT904_003181"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAP3192924.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(7870..8511)
                     /gene="udk"
                     /locus_tag="IT904_003182"
     CDS             complement(7870..8511)
                     /gene="udk"
                     /locus_tag="IT904_003182"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP3192925.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8829..12146
                     /locus_tag="IT904_003183"
     CDS             8829..12146
                     /locus_tag="IT904_003183"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP3192926.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRELNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAVASKGLSIALPLSVAGLSSTTLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLTGCRIVFSQVGRDLQIFNSLKVNMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(12184..13041)
                     /gene="alkA"
                     /locus_tag="IT904_003184"
     CDS             complement(12184..13041)
                     /gene="alkA"
                     /locus_tag="IT904_003184"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAP3192927.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKELGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            13175..>14027
                     /gene="yegD"
                     /locus_tag="IT904_003185"
     CDS             13175..>14027
                     /gene="yegD"
                     /locus_tag="IT904_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3192928.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPVDDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPDAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILP"
BASE COUNT         3269 a         3708 c         3634 g         3416 t
ORIGIN      
        1 cgggtcatac aggagcagtt tggtctgaaa acatcaatgt cgcgtaaagg taactgttac
       61 gacaacgctc cgatggaaag cttctgggga acgctgaaaa atgagagcct gagccactat
      121 cgttttaata accgggatga agccatctca gtaatacggg aatacattga gattttctac
      181 aatcgtcagc gtcgtcactc tcgtctgggg aatatctccc cggcagcctt cagggaaaaa
      241 tatcatcaga tgactgctta aaaaaagaac aaatggtagt gtccgctatt gccagtacac
      301 ctcagaccgc tttccctgca ctttaggaat tgtcgcacta agtgactatt cataaagcat
      361 tattgccgct actccgacca ttgcaccaca gaggccagag acaaactacc aaaagtctcc
      421 gaagccatac gtttctttac aatcgttata acattcgcgt tatgaataat ttctgcataa
      481 cgttatgagc ggcaggacga atacttcagc cttgccgctc agtttcaggc tggcgctcag
      541 gccagcagtt ttttaatgct ttcgcggaat ttggtgcctt ctttccggtt gcgcatcccg
      601 taggtgacga aggcctgcat atagcccatt ttcttcccgc agtcgtagct ttccccggtc
      661 atcagcatcg cgtcgacgga ctgcttcttc gccagttcgg caatggcgtc ggtcagttgg
      721 atacgccccc atgcgcctgg ctcggtacgt tccagttcgg cccagatatc cgccgacagc
      781 acgtagcgcc ccaccgccat cagatcggag tccagggtct gcggctcgtc cggtttttca
      841 ataaactcaa cgatacgcgc catctggccc tcagccacca tcggctcttt ggtctggatg
      901 acggagtatt cggagagatc gcccggcatg cgtttcgcca gcacctggct gcggccggtt
      961 tcgttaaagc gggcgatcat agcggcgagg ttatagcgca gcgggtcagc ggtgccaccg
     1021 tcaaggatga tatccggcag gactaccaca aacgggttgt cgccgactac cgggcgggcg
     1081 cagaggatgg aatgtcccag gcccagcggc tgcgcctgac gcacgttcat gatggtcacg
     1141 cccggcgggc aaattgcctg cacttccgcc agtagctggc gcttcacgcg ctgttccagc
     1201 agggcttcca gctcgtagga ggtgtcgaag tggttttcca ccgcattctt cgaggagtgc
     1261 gtcaccagaa caatatcttt gatcccggcg gcgacgattt cgtcaacgat gtactgaatc
     1321 attggcttat caacgatcgg cagcatctcc tttggaattg cctttgtggc cggcagcata
     1381 tgcatgccta gtcctgcgac cggaataacc gctttcaaat tcgccatatt catcttcacc
     1441 tgtttggttg caaaacacgc ggttttgcaa aattgtacat gtcacaaatt cattgaaact
     1501 gataatacac ggatactgcc cgagcatatg cccgccaaat tcttaaattc actgtcacgt
     1561 aaaagaatac acacaactcc cttttaccaa cagttaaaac tgagaataat cttattctcg
     1621 ttcaggttat tcagcggctc gttcctttgc tccgcacgat gtggctgaga tgcccgtcta
     1681 tcgggacctg ttggacgccg gacccgccgc gcgtatgccc gaaaagaata tatagataat
     1741 aaatttcgcc aggcaatata cttcagtata aacagccgga tatgacgaca tattagcctg
     1801 aatccgcggc tgtgaactgc gatccaccct attctgcact cacttcataa tatgaattat
     1861 gccggttttg tgagcgggct tcgcgcttcg cttctgctct gcgcatcagc gctcaccgtg
     1921 acgaaccgcg cgagtaagcc cttttgccac cctcgctgaa acgcagcgga taaagttctg
     1981 atatcggaaa gcttattcca tggtcgccgc catggtgtta ttttttcact gttcttttcc
     2041 ggaacataat tcttatcggt tcgcttaatc cgctggaaat atatccaccc acggcagagc
     2101 gatgtccgcg tcgttattgc ctgtataagt tcacagagat attgcaattg cctctggata
     2161 agtaagggga gattgcacta tgcaaatgca gcacctgatg gttggctatc ctaagtacta
     2221 ccaaacagcc gattatgcgt tgaggctttc agtgatggct gatatagcaa caatgagaat
     2281 gaaggctctg cacttctggg ataaacacga tatttctgca gcgtctgaag cctttggcgt
     2341 atcctgccgc acgccttact ggtggcgtca gttactgaac aagggaggac ctgaggggcc
     2401 aatcccgcac agtaaggcac ctctggtgcg acgaaaaaag cactggcatc ccgatgtgct
     2461 gaaagagatt cgacgcctca ggacagagct gccgaacctc ggtaaagagc agatttttgt
     2521 tcgcctgaag ccctggtgcg cacaacgcca cctgacctgc ccgagtgttt ccaccatcgg
     2581 cagaatgatc gccaccgcac acgataaaat gcggatgata ccagtacgtc tgagctcgcg
     2641 gggtaaggct ctgtttgtca aaaagcgaac caccaaaccc cgcaggccaa agcactatcg
     2701 tccggtaaag accggagagc tcattgggat ggacgccatt gagctcagaa tgggtgaaat
     2761 gcgtcgctat atcattacca tgatcgacga acacagcgat tacgcactgg cgctggctgt
     2821 gccgtcgctc aacagtgata tcgtcaatca ctttttcagc cgtgcagccc ggctgtttcc
     2881 tatcggtatc agccagatca tctcagataa cgggaaagag ttcctgggaa gctttgacaa
     2941 aacgctgcag gaagccgcca tcaaacacat ctggacctat ccctacacgc caaaaatgaa
     3001 cgctatctgt gatcgtttta accggacatt aagagagcaa tttattgagt ttaatgaaat
     3061 tttactcttt gaggatctgg cgttgtttaa ccagaagctg gcggaatatc tggtgctgta
     3121 taacagcaaa agaccccaca aggcgctcgc actaatgacg cccgtggaat atatgttaaa
     3181 agagaacaaa aattgcaata tgtggtggac ccatacattg cattttagac taatcttatc
     3241 catgatcgtg ataacacata ctgcagtcgt acaggtttta atacatatgg aatggattgc
     3301 cgatccgtca atctgggcag gtcttgtcac gctggtggtt atcgaactgg tgttgggtat
     3361 cgataaccta gtctttatcg ctatcctcgc cgagaagctc cctcccgccc agcgcgaccg
     3421 agcgcggatc actggcctga tgctggcgat ggtgatgcgt ctcctgctgc tggcctctat
     3481 ctcctggctg gtcaccctta ctcaaccgct gttcagcttc cgctcgttta cctttagcgc
     3541 ccgcgactta atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct
     3601 gaacgaacgg ctggaaggga aagatagcaa taaccccacg caacgcaaag gtgcgaagtt
     3661 ctggggcgtg gtgacacaaa tagtggtgct ggacgctatc ttctcactcg actcggtgat
     3721 taccgccatc gggatcgtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat
     3781 cagcctgatg ttgatggcca gcaagccgtt aacccagttc gttaacagtc acccgacgat
     3841 cgttattctc tgcttaagct tcctgttgat gattggcttt agcctggtgg cggaaggttt
     3901 cggcttcgtc attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga
     3961 ggcgttcaat cagttggcta tctttaaccg gcgacgtttt ctttccgcta accagacact
     4021 gcgccagcgg actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga
     4081 actggacgcc gaaaccgcgt ccatgctggt ggatcatggt aaccagcaga tctttaatcc
     4141 gcaggaacgg cggatgattg agcgggtact taatcttaac cagcgcaccg tcagcagcat
     4201 tatgacgtcg cgccacgata ttgagcatat cgatctcaac gcgccggaag acgagatccg
     4261 ccagttgctg gagcgaaatc agcatacgcg gcttgtcgtt actgatggcg atgacgcaga
     4321 agatttgctc ggtgttgttc acgttatcga cctgttacaa cagtcactgc gcggcgaacc
     4381 gctcaacctg cgggtgttga ttcgtcagcc gctggtgttc ccggaaacct tgccgttgtt
     4441 acctgccctg gagcagttcc gtaatgcccg cacacacttt gcttttgtgg tggatgagtt
     4501 tggctcggtg gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt
     4561 accgaacgaa gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc
     4621 ctggacggcg aatggtcata tgccgctgga agatctggtg caatatgtgc cgctgccgct
     4681 ggatgaaaaa cgtgaatatc acaccattgc cgggctattg atggaatatt tgcagcgtat
     4741 tccaaaacct ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtaga
     4801 aagccatcgc gtgcagaagg tgcagattat tccgctgcgt aaggatggcg agatggagta
     4861 cgaggtgtga tggtatcggc cattttaccc aatgctgcgt aacgccttat aacttcagca
     4921 atgaagcgtt gtctgtggta catggacatc acagcggggg tgtgggtccg gctgtaaatc
     4981 gccgaatcgt ttcctgtagg ccggggcagc ccacatggat gtgggctgag ggcgcgtttt
     5041 acagtgatgt tacctcgcgc ccgtacccga tagccgaaag gaataagatg agggaaccac
     5101 gccagcggcg atttaccgcc gggagcctgg gttgccaggg tggtggcgat tgagccaccc
     5161 tggcacgttc accggcttga gcgttccata gtagcgagga acatcaagtg aacggaacag
     5221 ccttcgatct tacatattca gaatataatg gtgccgccac gaacgcctga tgcgacgctt
     5281 tacgcgtctt atcaggccta cagaacaggt cacctggtaa cctacatctt ctccagcaac
     5341 ttcttcacat ctttgccatt gcgggaatct ttattccgct cggcccagtc gttaagacgg
     5401 cgttttgctt catcctgtag atgtttacgc agtaactgat ccacttgcag gctgtaatta
     5461 agttgctgcc agttgccata aacccgcagc ggtactggcg tttctttgag gaaatcaatc
     5521 agtttgcttt ctccgttcca gccacccacg acccgaatat caaactgggt gtcgcaggtt
     5581 tgctccgcca gattcaacgt accagccccg gaaagcgcca gcatgggcga ttgaccttgc
     5641 atgtcgttta acgtcacgac gccatccttc aacgtcaaat cggtggtaaa gcggtcaaga
     5701 cgcgtcacgt tatcgaagtt ttcagcggcc ttcacatcac caccattacg ttctaccgct
     5761 tgctgaatca tctgctggaa gttcatccct tccatgcgtg tgtcggtcat ttcgacatgt
     5821 gcctgtcctt gccagttgtg gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca
     5881 ccagccagtg acatctttcc ggtcaacgaa atcggatagt taaacgcctt cagaatggtg
     5941 ccaatctcaa cgttttccag ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg
     6001 tccaacgtgc ccggcagtga aacctgtcca ccgttaagtt tgccctgcag ttgagtaatt
     6061 tccagcaaac ccgacttgtt ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc
     6121 cagcgcacgt tactggcctg caacaaaata tcagccgtaa agccttgcag tccctgatag
     6181 gccggttcat caatacgcga agaaatgacc gggcgcggca acgttgattg gctcagccct
     6241 tgctgcgcgg caccgttctc accattcgct gtttcattaa aggggatgag gttgtcaaga
     6301 ttcagttgcg ggaattgcag tctcagctgc cattccggtt tctctgtcaa cgtgacctgt
     6361 gcttgcccgc tcagcgtact gtcattggcg gtcaaactaa tttggttaaa tgaaaggcgt
     6421 ttatgtgact cctgccactg gacctggaaa ctcccctgcc cctgaatacc ttgttttggt
     6481 aaatcggcac cctgcaactg ccagttaatt tgttcaatag ccgccattaa atcatgcgga
     6541 taatcagaag catccaccgt accgttaagg gatatcgtga gatcgcgctg atcgcgatta
     6601 acccgcccgg agaactcaaa tgagccacga tgttggggat cttgttccat ttgcaggcgg
     6661 atattgcgga ttgtcacctg ctcgtcatct tcatgctgga acaccagaac gctgtccgcc
     6721 accttaagac tggatatatc aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta
     6781 ttgtcgcgcg gtgcaaccgg agcgtcttca ctgcgcacag cttccgtctg cggcgtcagt
     6841 tggatcactg ccccctttag catcacctgc ttaacgctca gttgatgact cagtagtggt
     6901 aaaagcgcca cgtccagacg catgttgtag gcgcgaacca gtggctggct tgcgccctgg
     6961 gcggtgagag acattcgccc ggagaggata ctaagctgcg gccagacgtg ccaacgcagt
     7021 ggcccgtcga gctgcaattg ataaccgcta cgtgcagcaa cttgcttgac catatagtcg
     7081 cggaaatcat tcggattcac cagcaatact aacgcagata acccggccac cagcacgacc
     7141 aggagtatca tcagcgtcgt cagaaatcgt ctcatggtat cctcaatggg cctgaattag
     7201 tctttatcga ttcggctggc taccgcgccc tgctggttgc gatatttcgc atcttcacgg
     7261 cggttgtaag gtcgcgccgc cgggccggag agcggctcaa agctcagcgc accaattaac
     7321 atgcccggac gcagcgccag cggcagctta ccggagttgt aaaactccag cacaatgcag
     7381 ccagaccagc ccggatcgat acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc
     7441 agtgaagaac gcccgtccag ccagcccacc agatcggctg gcagcgtcac cgactccagc
     7501 gtcaccgcca gcgccagctc tccagggtga agataaaacg cctcgccctc gtcgagcacg
     7561 atttcatcgc tcatcacgcg gtcaagcgcg gcgctaactt catctttggg accgctcaga
     7621 tcgataaacg ctgccgtgtg accacggaag gtacgaaatt tattgcccag gcgcacatcc
     7681 accgtcgcgc cgttaatacg ctccactggc ggacgtgggt tgatcgacaa acggccttca
     7741 tcaagccagg cttcaatatc tcggtcacac agacgcatgg cattttctcc tttcgcgcat
     7801 cactccctta acgccaatta cgtcaagggc ataccaggtt atcactgaac ggtacacaat
     7861 ttatcaagct tattcaaaga actgacttat tttcgctttc aatatatcga tcgcgatgcg
     7921 gtttttcccg ccgcgcggca cgataatgtc cgcatattgt ttagaaggct caatgaattg
     7981 caggaacatc gggcgcacgg ttttttgata ttgcgccatc actgaatcca ttgaacgccc
     8041 acgctcgtta acgtcacgct tgatgcggcg catcaggcag atatccagcg gggtatcaac
     8101 gaaaattgag aagttaagtt cgtcacgcaa acgcgcatcc gttagcagca aaatgccttc
     8161 gagaatgatg actttcttcg gctcaaccgt caccgtttct ttcatacgcg tatgttcaac
     8221 atagctgtaa accggcaggt caattgccga gccgcgtttc aacgcttgta aatgctcaag
     8281 cagcaggctg tgatccatcg cgctgggatg gtcgtagttg gtcttaacgc gttcttccat
     8341 cgacagatgg ctttgatctt tgtaatagca gtcttcggga attacgccga tgtgttcatc
     8401 accgacttgc tcacgcaatt cacgataaag ggtactggca ataagactct tgccggaagc
     8461 cgatgcgcca gcgataccga taatgacgca ctgatgagac tgatcagtca tatatttagc
     8521 gacctgatta acctggatgt taggaagggg gcgacgaagc gccaaacgcg gcaattatag
     8581 ggatttcatc cgcctgatac cagtcgaata gcgttgccgc gcgctcagag ttaattgttg
     8641 acgaagaatt cccggtggca aattacgttg atcagtttta tacaaggtaa aaaatgttat
     8701 acgccgttgc gcaaattatc cgcctttacg tcactttatg agcaattcgc atataaaatg
     8761 taaaactttt gtactagcat aaacacagaa acgaatactg gcgacctggt cttgcggata
     8821 aagcggtaat gagcaaacaa tcacagcatg tattaattgc cctgccccac ccgctgcttc
     8881 acctggtcag tttaggttta gtctcgttta tctttaccct tttctcgctt gagctttcgc
     8941 agtttggcac ccaactcgcc ccactgtggt tcccgacgtc catcatgatg gtggcgtttt
     9001 atcgccatgc cgggcgcatg tggccgggga ttgcgctgag ctgctcgctg ggaaatatcg
     9061 ccgcatccat cctgcttttt tccaccagct cgctgaacat gacctggacg accatcaata
     9121 ttgttgaagc cgtggtcggg gcagtgctac tgcgtaaatt gctgccgtgg tataacccat
     9181 tgcaaaatct ggctgactgg ctgcgtctgg cactcggcag cgccattgtt ccacctctgt
     9241 tagggggtgt tctggttatc ctgctgacgc ccggagacga tcctctcagg gcatttttga
     9301 tatgggtact gtcagaatcc atcggcgcac tggcactggt gccgctggga ttgttattta
     9361 aaccacacta tctgctgcgc catcgcaacc cacggttgct ttttgagtcg ctgctcacgt
     9421 tagccatcac actgacgtta agctggcttt cgatgctgta tctgccgtgg ccttttactt
     9481 tcattattgt gctgttgatg tggagcgccg tgcgcctgcc acgaatggaa gcctttttga
     9541 tcttccttac cacggtgatg atggtgtcac tgatgatggc cgcggatccc tccctgcttg
     9601 ctacgccgcg tacatacctg atgagccata tgccgtggct accgtttttg ctgatcctgc
     9661 tgcccgccaa catcatgacc atggtgatgt atgcctttcg tgcggaacgc aaacacattt
     9721 ccgaaagcga aacccgcttt cggaacgcga tggaatattc cgccatcggc atggcattag
     9781 tgggcaccga ggggcaatgg ctgcaatcca acaaagcgct ctgccagttt ctcggttaca
     9841 gtcaggaaga actgcgcgga ctcacctttc agcaactgac ctggccggag gatctcaata
     9901 aagatctgca acaggttgaa aagctgatca gcggtgaaat aaacacctat tcaatggaaa
     9961 aacgctacta caaccgcaat ggcgatgttg tctgggcgtt gcttgccgtc tcactggtgc
    10021 gccacaccga tggtacgccg ctctatttta tcgctcagat tgaagacatt aacgagctaa
    10081 aacgcaccga acaggtgaat cagcaactga tggagcgcat cacgctggct aacgaagcgg
    10141 gcgggattgg catctgggag tgggagctta agccgaatat ttttagctgg gataagcgga
    10201 tgttcgagct gtatgaaatt cctccgcata tcaaaccaaa ctggcaggtg tggtacgagt
    10261 gcgtgctgcc ggaagatcgc cagcacgccg aaaaagtgat tcgcgattca ttgcaatcgc
    10321 gctctccctt taaactggaa tttcgcattg ccgtgaaaga tggcatccgc catatccgcg
    10381 ccctcgccaa ccgggaactg aataaagaag gcgaagtcga acgtctgctc ggcattaata
    10441 tggatatgac cgaggttaaa cagcttaacg aggcattgtt tcaggaaaaa gagcgcctgc
    10501 acattacgct tgattccatc ggtgaagccg tggtctgtat tgatatggcg atgaaaatta
    10561 cctttatgaa tccggtggcg gagaagatga gcggctggac gcaggaagaa gcgttaggtg
    10621 ttccactcct gacggtgttg catattactt ttggcgacaa cggaccatta atggagaaca
    10681 tttacagtgc cgacacctca cgttccgcga ttgaacaaga tgtggtgttg cactgccgaa
    10741 gcggcggcag ctacgacgtg cattacagta ttacgccgtt aagtactctg gacggcagca
    10801 atattggttc ggttctggtg attcaggacg tcaccgaatc gcgcaaaatg ctgcgccagc
    10861 tgagctacag cgcctcccat gatgcactga cgcatctcgc caatcgcgcc agttttgaga
    10921 agcaactacg catcctgctg caaacggtaa acagtacaca tcagcgacat gcactggtgt
    10981 ttatcgatct tgatcgcttt aaagcggtga atgacagcgc cgggcacgcc gctggcgacg
    11041 ctttgttgcg cgaacttgcg tcattgatgc tgagtatgct gcgctccagc gacgtgctgg
    11101 cgcgactcgg tggcgatgaa tttggtctgc tgctgccaga ttgtaatgtc gaaagtgcgc
    11161 gttttatcgc tacacgcatt atcagcgccg taaatgacta tcactttatc tgggaaggac
    11221 gtgtacatcg ggtaggtgcc agtgccggga ttaccttgat tgatgacaac aatcatcagg
    11281 cggctgaagt gatgtcgcag gctgatatcg cctgttatgc ctccaaaaat ggtggacggg
    11341 gccgggtgac ggtttacgaa ccgcagcaag ctgccgcaca tagcgaacgg gcggtgatgt
    11401 cgcttgatga acagtggcgg atgattaaag agaatcagtt gatgatgatc gcccacggtg
    11461 tcgcttcgcc gcggatcccg caagcgcgta atttgtggct gatttcactt aagctctgga
    11521 gttgcgaagg cgagattatt gatgaacaaa catttcgacg tagcttcagc gatccggcac
    11581 ttagccatgc tcttgaccga cgggtattcc acgatttttt ccagcaggcc gcaaaagcgg
    11641 ttgccagtaa aggcttaagc atcgccctcc ccctttccgt tgccggtttg agtagcacca
    11701 cactggtaaa tgaactgctt gaacagctgg aaaacagccc tttaccacca cggttattac
    11761 atctgattat tccggcagac gcgattttag atcacgcaga aagcgtgcaa aaactgcggc
    11821 tgacgggatg tcggatcgta ttcagtcagg tgggccgcga tctgcaaatc ttcaactcgc
    11881 tgaaagtaaa tatggcagat tacctactac ttgatggtga gttatgcgcc aacgtgcagg
    11941 gaaatttgat ggatgagatg ctgattacga tcattcaggg gcacgctcag cggctcggga
    12001 tgaagaccat cgccgggcca gtagttttac ccctggtgat ggatacgctt tctggcatcg
    12061 gcgtcgattt gatttatggt gatgtgattg ccgatgccca accgctggat ttgctggtga
    12121 atagcagtta tttcgcgatt aactaaggcg tgcttcccca tcgcctgatg cggcgtatca
    12181 ggcctacaat tcgtccgttc cgtctggttg ccagccttcc gtataccaga tatgcaacag
    12241 cgcataagaa cgccagggct tccagcgttc ggcataacgg cggatttgtg ccggtgtcat
    12301 tcccggaaat cgctgtttaa tcagataatc atccggcagg aaaacatctt tcgcctgcca
    12361 gccgcgcaag gcgaaataat tcgccgtcca gcgcccgata cccggaaaag tttgcagcgt
    12421 tttcatcgcc tgctccacat cgcccggtat tgtcattggt aaggtgccct ccagcgccgc
    12481 atttgccaga tgaatcagcg cctctgcccg tttcaacggc atacctaact cttttaatgc
    12541 ctgcggatct gctgctgcca gccgctgagg cgtggggaag cagacataat ccggaaaatc
    12601 atccagccgt tcgccataaa gctgtgccac tctggcggtc aattttgccg ccatcgccac
    12661 gctcaccagt tggcctaaaa tcgcccgcac gccctgctca aaagcatcaa cgctgccggg
    12721 taaacgcaat ccgggccgcg ccgcgcctaa cttgcccaac gcaccgttaa caatctgcgg
    12781 gttacattgc agatcaaaca ggcggctcat tttcgccaga cactctgcgg caacaggttc
    12841 taaacctgca cttaaattta tgtgcagagt atggcgggct atatccggaa tagcagtcac
    12901 cacgccgcga tattcgccca ccgccagact acgggcataa taatcgtcag cgaccgtttc
    12961 cacaccgttc accgcacggg cggcgagaaa tcccaacatc cacgaccagt catatggcgg
    13021 ctgccagttc agggtataca tcgcatctcc ttgttaatcc gctttcagca taaacgttat
    13081 tcagacgcta cgctttgctt tcatattccg gttgtcgcga cggcaacatt tcgctaaagt
    13141 cacgcccctt cttcaccggc atggggatta tttcgtgttt attggttttg attacggtac
    13201 agcaaactgt tcagtggcgg tcatgcgcga cggtaagcca caattactga aaatggaaaa
    13261 cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaagcgg taagcgaatg
    13321 gctgtaccgc catcatgatg ttccggtaga cgacgatgaa acgcaggcgc tgttgcgtcg
    13381 ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg tgcagttcgg
    13441 tctttcctca ctggcacagt acattgatga tccagaagag gtgtggtttg tgaaatcacc
    13501 aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt ttgaggatct
    13561 ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcgcagctgc cggatgcgat
    13621 tactcaggcg gtgattggtc gcccgatcaa cttccagggg ctgggcggcg atgaagcaaa
    13681 cgcccaggcg caggggattc tggaacgcgc ggcgaagcgt gccgggttta aggatgtggt
    13741 attccagtac gagccggtag cggctgggct ggattacgaa gccaccttgc aggaagaaaa
    13801 acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc tgctgatggg
    13861 gccgcagtgg cgttcgcgtc tcgatcgtga agccagtctg ctgggtcaca gtggttgtcg
    13921 tattggcggt aacgatctgg atatcgccct ggcgtttaaa aacctgatgc cactgctggg
    13981 tatgggtggc gaaaccgaaa aaggcatcgc cctgccgatc ctgccgt
//
LOCUS       DACAPB010000123        13932 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.123, whole genome shotgun sequence.
ACCESSION   DACAPB010000123 DACAPB010000000
VERSION     DACAPB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.123"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..312)
                     /locus_tag="IT904_003186"
     CDS             complement(<1..312)
                     /locus_tag="IT904_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192929.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SL"
     gene            838..1812
                     /gene="hemB"
                     /locus_tag="IT904_003187"
     CDS             838..1812
                     /gene="hemB"
                     /locus_tag="IT904_003187"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAP3192930.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFAMDLAEKKILR"
     gene            complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="IT904_003188"
     CDS             complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="IT904_003188"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAP3192931.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2818..3645)
                     /gene="tauC"
                     /locus_tag="IT904_003189"
     CDS             complement(2818..3645)
                     /gene="tauC"
                     /locus_tag="IT904_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAP3192932.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAARSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="IT904_003190"
     CDS             complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="IT904_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAP3192933.1"
                     /translation="MLQISHLYANYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQNNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4422..5384)
                     /gene="tauA"
                     /locus_tag="IT904_003191"
     CDS             complement(4422..5384)
                     /gene="tauA"
                     /locus_tag="IT904_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3192934.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            6000..6671
                     /locus_tag="IT904_003192"
     CDS             6000..6671
                     /locus_tag="IT904_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAP3192935.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            6681..7856
                     /locus_tag="IT904_003193"
     CDS             6681..7856
                     /locus_tag="IT904_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP3192936.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RAVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTLVANGYRI
                     RQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIFPMLLVVI
                     YGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRYWLLVPLAPLS
                     VVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGTEKLSEA"
     gene            7834..8415
                     /locus_tag="IT904_003194"
     CDS             7834..8415
                     /locus_tag="IT904_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP3192937.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            8417..9190
                     /gene="yaiO"
                     /locus_tag="IT904_003195"
     CDS             8417..9190
                     /gene="yaiO"
                     /locus_tag="IT904_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HAP3192938.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDVWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            9378..9653
                     /gene="frmR"
                     /locus_tag="IT904_003196"
     CDS             9378..9653
                     /gene="frmR"
                     /locus_tag="IT904_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HAP3192939.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            9688..10797
                     /locus_tag="IT904_003197"
     CDS             9688..10797
                     /locus_tag="IT904_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HAP3192940.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSIKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            10889..11722
                     /locus_tag="IT904_003198"
     CDS             10889..11722
                     /locus_tag="IT904_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase FrmB"
                     /protein_id="HAP3192941.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(11948..12487)
                     /locus_tag="IT904_003199"
     CDS             complement(11948..12487)
                     /locus_tag="IT904_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HAP3192942.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(12589..13800)
                     /gene="mhpT"
                     /locus_tag="IT904_003200"
     CDS             complement(12589..13800)
                     /gene="mhpT"
                     /locus_tag="IT904_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HAP3192943.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
BASE COUNT         3468 a         3654 c         3405 g         3405 t
ORIGIN      
        1 cagactattg gcgttggtat tggtagaaat accgtcgtcc agatagtagg tagaaatcac
       61 gccgttaaca gtagcgttat taatgaccgg gtagatatcg ccgttgtaat aactatcagc
      121 ggcatagttg ttccagccta cataaccatc atagtaaacg tcatcagcat aggtggcgtc
      181 gttgtagtga tggaaagtat tgtaagtttg accagaaata tcgttggtcg ctgcattagc
      241 ctgagaggtg atagccagag tgcaagccaa tgctaattgt gatactacaa gtttcttttt
      301 ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gtttatccat caattatcaa
      361 tgcatagaaa acatattatg tttcgcgcta acgattatgc tgcgttacag acgaatggtt
      421 caattattct ttcccctaag tccgaatgtg gacatatata atttatggag aaaaatacac
      481 atcaaattga taaaaacacc atatttcaat atgttgcaga taatgttatt tttgaaacat
      541 acgcatcata aatatcacag acacaaacca gacaaatatc ctatcaatat gcaacaaagt
      601 tttaattatt caatttatag cagcacaacc aaacgcaaaa aaaaataata accaagagct
      661 ataaataaag attaaaaata ttttcagtga atatgaaatt attgctattt ctattacaaa
      721 gatgtcctgg tctctctttc attctcttct tgtcaacatc acgacaactt tcgtaaaaca
      781 ctcctgcccc gcttcaggta tactgtgcac cttaattcca caaacatcag gcagaccatg
      841 acagacttaa tccaacgccc tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt
      901 gaagagacaa cacttagcct taacgacctg gtgttgccga tctttgttga agaagaaatt
      961 gacgactaca aagccgttga agccatgcca ggtgtgatgc gcattccaga gaaacatctg
     1021 gcacgcgaaa ttgaacgcat cgccaacgcc ggtattcgtt ccgtgatgac tttcggcatc
     1081 tctcaccata ccgatgaaac cggcagcgat gcctggcggg aagatggact ggtggcgcga
     1141 atgtcgcgca tctgcaagca gaccgtgcca gaaatgatcg tcatgtcaga cacctgcttc
     1201 tgcgaataca catctcacgg tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac
     1261 gcgactctgg aaaatttagg caagcaagcc gtggttgcag ctgctgcagg cgcagacttc
     1321 atcgcccctt ctgccgcgat ggacggccag gtacaggcga ttcgccaggc gctggacgct
     1381 gcgggcttta aagatacggc gattatgtcg tattcgacca agttcgcctc ttccttttat
     1441 ggtccgttcc gtgaagctgc cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg
     1501 aacccaatga accgtcgtga ggcgattcgt gagtcactgc tggatgaagc ccagggcgca
     1561 gactgtctga tggttaaacc tgccggagcg tacctcgaca tcgtgcgtga gctgcgtgaa
     1621 cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg agtacgcgat gattaagttc
     1681 gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc tcgaaagctt aggttcaatt
     1741 aagcgtgcgg gtgcggatct gattttcagc tactttgcga tggatttggc tgagaagaag
     1801 attctgcgtt aattttgttt cctctcagaa agggggagtt aacctgttga aagtacggtg
     1861 aacgccccct caccctaacc ctctccccaa gggggcgaag ggactgtccg tgctcgcttc
     1921 atctatatca ccgttctttc gccgctcgag gtgaccgtcc actctcgtat caccccgccc
     1981 gataaaacgg tttatcccca agaatcgtcg cccgatgcat aatccgtcgc tgtggcaggt
     2041 aatcggcatt ggcatagtgc tgggtcacgc ggttatccca aatcgcaata tcatttggtt
     2101 gccagcgcca gcgcacctga aactccggtt tagtgatatg agcgaataaa aaacttaaca
     2161 aggcttcgct ctctttctcg ctcacatcaa caattcgcgt agtaaagcct tcattcacaa
     2221 acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggcggat
     2281 ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc ggttttgcgg tatttgtatt
     2341 ccgggaacga tttacggaaa tcatgctccg cacgcagccc gctcagcagc tggcggaagg
     2401 gaacagagag cgcctcatag gccgcaatac cgctggtcca gagcgtatca ccgccggtcg
     2461 aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat
     2521 cggtatgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct
     2581 cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg
     2641 ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg cagaaatacc acctgatggc
     2701 gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg
     2761 cacccgaaat ttgtgcgccg atatacggcc ccagcggggt aatgctcaaa cgttcactca
     2821 ttgtacttct ccatgccagg gcgtcaggcg gcgctgtaat gcgcgcagac ccagttctaa
     2881 aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagc accacgtcag tcgcgagaaa
     2941 ttcacccgct gactgaacca taaatcctaa accgcgcgtt gcggcaatca gctccgccgc
     3001 caccaacgta gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag
     3061 cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgaccgggc
     3121 ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc agcgccgaca ttgccaccgg
     3181 cgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcgccaatac caaaccaaat
     3241 caccatcagc ggcaaataag ccagcggtgg caccggacga taaagctcga ttatcggatc
     3301 cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgattac
     3361 caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg gcggccagat gctgccacag
     3421 cgtggcgtcc ataaatccct gcggcccggc aatggtgagc agttttgcca gtacctgttg
     3481 cggtggcggc aaaaacagcg ggctaatcag ttgcagcgct gcgactgccc accatacagt
     3541 gagcaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     3601 tttcagccga tgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgc
     3661 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3721 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3781 agcacgcggc cagggccggg tgaaagcaga accagttcag tcgccataaa caccgcttct
     3841 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3901 agggtttgca tctggtcgcg ggtgaaggca tccagcgcac caaacggttc gtcgagtaat
     3961 aacagctggg gatttgccgc cagcgcgcga gcaatgccca cccgctgacg ttgaccgccg
     4021 gagagctgcc agatgtagcg tttttctgct ccttccagcc ccactttttt cagcatctgg
     4081 tgcgcgattt ccagtcgctg cattttctct atacctgcca gttgcaggcc gaacgccacg
     4141 ttgttctgga cattgcgcca cggtagcagc ccttcattct gaaaaaccac gccgcgctct
     4201 gctcccggcc cctcaatacg cttacccgcc agttgaatgc tgccgtgctg ataaggcaca
     4261 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     4321 agtagctcac cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     4381 ttggcgtaaa gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     4441 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     4501 tgcgcggtgt cgatgatcgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     4561 ggcgtcagat aggtattccc cttcaccagc cccggaacgt caccttcagg cacgccgctt
     4621 aaccgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtctgg gttagcaatg
     4681 tacggttgct gagcatcgat ggcgcttttg gcgaacgctt tcaccacctc aggatgtttc
     4741 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4801 tgttcagaat cggtcagcac tttgccgtct ttttccaggg cgttaaccgc cggtgcccag
     4861 acataagcac catcaatatc tccccgctgc catgcagcga taatcgcggg cggctgcagg
     4921 ttcacaatct ccacttgccc aggtttaatg ccccagtgtt tcagcgctgc cagcaggctg
     4981 tagtgggtgg tagagataaa cggtacggcg atgcgtttgc caatcagatc ttccggtttg
     5041 ctgatagttt tctttaccac cagcgcttct gagttaccca gttttgacgc cagcaagaag
     5101 acttcaatcg gcacctgttg gctggctgca accgctaacg ggctggaacc aaggttgccg
     5161 atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     5221 cagtccacgg ttgctccgct ttctttagca aaggtgttgt cggcctgagc cactttcgcc
     5281 ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg
     5341 aatgccagtg cggcaagaag tgtgtttcgc gatgaaattg ccatgattgt ctgctcccct
     5401 gtcttgttat gggagcagta ttcaggaata aaaacattca ttaaaagaat tagtcgttat
     5461 cgcacagatg attttattct tagcaaaaaa acggctaagg aaagattaaa tatgcaatta
     5521 ctccgttcta acaattggga aaattactac acttcatttt agtgatgcat aaacaaccac
     5581 attacgagat ttattacttc attatttcta tcaatatcaa tacatataat aaaactatta
     5641 tattcgcatt gtatatatca ctgtatcatt ctcaatcact cgcatattac attcacataa
     5701 tgtatataaa caatgcttgt tcacagaaca tattttttaa atgcaattaa ttgacaaact
     5761 aaatcacatc taaatacaat gtataaaaca aaataactat acataccaaa catacaaaca
     5821 cttaacatat gaattgcata gaaacattac ccagtcgaac ccgcgggtct gaaagccctt
     5881 gcttttcagt tattagcaac atttaacatt tataaattaa catctggctg cgccagagat
     5941 tacgtctctg agtgcatcgt tgtgcttgta gtataataaa taaaatggag tgtcattaag
     6001 tggataaggt tttagattca gccctccttt cttcggcaaa taaaagaaag ggtatccttg
     6061 ctattggcgc acatcctgac gatatagaat taggctgtgg cgcatcgctt gctcgtcttg
     6121 cgcaaaaagg aatttatatc gcagccgtgg tgatgactac cggcaactct ggcacagatg
     6181 gaataatcga tcgccatgaa gaatcgcgca acgccctaaa gatattaggg tgccaccaaa
     6241 ctattcatct taattttgct gacacccgcg ctcatttaca gctcaatgat atgatttccg
     6301 ccctggaaga catcattaaa aatcaaattc cttctgatgt tgaaatcatg cgggtatata
     6361 ccatgcatga tgccgaccgc catcaggatc atctcgctgt ttatcaagct tcaatggttg
     6421 cctgccgcac tattccacaa attctcggct acgaaacccc aagtacctgg ctttcattta
     6481 tgcctcaggt ttttgaatcc gttaaagaag aatatttcac agtcaaactt gctgcattaa
     6541 aaaaacataa aagccaggaa cgacgcgatt atatgcgtca tgatcgcctg cgtgcagttg
     6601 cacaatttcg cgggcaacag gtcaatagcg atctgggtga aggctttgta attcataaaa
     6661 tgattctttg agcaaattgc atgaaaacct ggatatttat ctgtatgtcc atagcaatgt
     6721 tgctatggtt tttaagtacg ctaagacgta aacccagtca aaagaaaggc tgtattgacg
     6781 ccattatacc tgcgtataac gaaggcccgt gtctggcgca gtcactggat aatctactgc
     6841 gtaaccctta tttttgccgg gtaatttgcg ttaacgacgg ctccacggac aataccgaag
     6901 cggtcatggc ggaagtcaaa cgcaaatggg gcgaccgctt tgttgccgtc acgcaaaaaa
     6961 ataccggtaa aggtggtgcg ctgatgaatg gcctcaatta tgccacctgc gaccaggttt
     7021 ttttaagtga tgccgacacc tatgttccgc ccgatcaaga cggaatgggc tatatgctgg
     7081 cagaaattga gcgcggtgct gatgccgtag gcggcattcc ctctactgcg ttgaaaggcg
     7141 cggggctgtt accgcacatc cgcgctgtta tgaagcgcac gctacagcag ctcctgggcg
     7201 gcgcaccgtt tattatcagc ggtgcctgcg gaatgttccg tactgatgta ttgcgtaagt
     7261 tcggtttctc agatcgtact aaagtcgaag accttgatct cacctggaca ttagtggcaa
     7321 acggctaccg tattcggcag gcgaatcgct gcatcgtata cccacaggaa tgcaacagtc
     7381 cgcgtgagga gtggcgtcgc tggcggcgtt ggattgtggg ctacgcggtc tgtatgcgcc
     7441 tgcataaaag gcttttattt agccgcttcg gtatcttcag tatatttcct atgctgttgg
     7501 ttgtgattta tggcgttggg atttatctca ctacctggtt taatgaattc atcaccaccg
     7561 ggccgcatgg tgtggtgttg gcaatgtttc cgcttatttg ggtcggcgta gtttgtgtta
     7621 ttggtgcttt tagcgcctgg tttcatcgtt actggttgtt ggtgccttta gcgccgcttt
     7681 ccgttgtgta tgtattatta gcttatgcca tctggattat ttatggactt attgcctttt
     7741 ttactggacg cgaacctcag cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt
     7801 caaccgctta ttcccaacct tctgtcacag gaactgaaaa actatctgaa gcttaatgtt
     7861 aaagagaaaa atattcagat tgcagaccag gtaattattg atgaaactgc aggtgaagtt
     7921 gttatcggcg cgaatacccg tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt
     7981 ggcgcaaact gcctgatagg taattatgcg tttattcgtc ctggcacaat aatcagcaat
     8041 ggtgtaaaaa ttggttttgc caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg
     8101 attggtccgc aatgttttat tgccgactcg gtagttgcaa accaggcata tttgggcgca
     8161 caagtacgta ccagtaatca tcgtctggat gaacagcctg tgtctgttcg aactccagag
     8221 ggaattatcg ctaccggatg cgataaatta ggttgttata tcgggcagcg ttcacgcctt
     8281 ggcgtacaag ttataatttt gcctgggcga attatttccc cgaacacgca acttggcccg
     8341 cgcgtgattg tagaacgtaa tttacctgcc ggaacttatt cacttcgaca agaacttatc
     8401 cgtacaggag attaaaatga taaaacggac gttattagcg gcggccattt ttagcgcatt
     8461 gcccgcttat gccgggttaa cttccattac cgcgggctac gattttaccg attattctgg
     8521 cgatcatggc aaccgtaatt tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac
     8581 gctgcttttt aatctttccc aggggcgtcg tgattatgaa actgaacatt tcaatgccac
     8641 tcgcggtcag ggtgcggtct ggtataaatg gaataactgg ctgacaaccc gaacgggtat
     8701 tgcctttgcg gataatacgc cggtctttgc ccgccaggat tttcgtcagg atattaacct
     8761 ggccctgttg ccaaaaacgc ttttcacgac cggttatcgc tacactaaat attacgatga
     8821 tgtcgaagtc gatgtctggc aaggcggcgt atcactctat actggcccgg tgatcaccag
     8881 ctaccgctat acccattatg actccagcga tgcaggtggt agttatagca atatgatttc
     8941 cgtgcgtctg aatgacccgc gcggcactgg ttatacgcaa ctatggctaa gccgcggaac
     9001 aggcgcttac acctatgact ggacgccaga aacacgctac ggcagcatga agagcgtcag
     9061 tctgcaacgt attcaaccgc tgactgagca acttaatctc gggctgacgg caggtaaagt
     9121 gtggtacgac accccaaccg atgattataa cggtctgcaa cttgcagccc atctgacctg
     9181 gaaattctga ttccttctgc cgcccgctat ccggggcggc cttccctgcc gattagcccc
     9241 cccccctttc ctctttgttt tccgaccaca ttcaccggat aaattttatt ctccagtgtt
     9301 atatactata ggggggtatg cattgacata tagcataccc ccctatagta tattgcgtgc
     9361 agataatgag gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc cttactcgag
     9421 ttcgtcgtat tcgagggcag attgatgctc tggaacggtc gctggagggt gatgccgaat
     9481 gccgtgccat actccaacag atcgctgccg ttcggggcgc ggctaatggg ctgatggcag
     9541 aagtgcttga aagccatatc cgggaaacgt ttgaccgaaa tgactgctac agccgcgaag
     9601 tcagccaatc cgttgacgac actattgaac tggttcgagc ctatcttaaa tagctgaatc
     9661 tattaccata ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc atttgctccc
     9721 ggtaaaccgc tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg tgaagtgctg
     9781 attaaagtca cccataccgg cgtttgccat accgacgcat ttaccctctc cggggatgac
     9841 ccggaaggtg tattcccggt ggttctcggt cacgaagggg ccggcgttgt ggttgaagtc
     9901 ggtgaaggcg taaccagcat caaacctggc gaccatgtga tcccgcttta caccgcggag
     9961 tgcggcgagt gtgagttctg tcgttctggc aaaactaacc tctgtgttgc ggttcgcgaa
    10021 acccagggta aaggcttgat gccagacggc accacccgtt tttcttacaa cgggcagccg
    10081 ctttatcact acatggggtg ctctacattc agtgaataca ccgtggtcgc ggaagtgtct
    10141 ctggccaaaa ttaatccaga agcaaaccat gaacacgtct gcctgctggg ctgtggcgtg
    10201 accaccggta ttggcgcggt gcacaacaca gctaaagtcc agccaggtga ttctgttgcc
    10261 gtgtttggtc ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca ggcgaaagcg
    10321 ggtcggatta tcgctattga tacgaacccg aagaaattcg atctggcgcg tcgcttcggt
    10381 gctaccgact gcattaaccc gaatgactac gacaaaccga ttaaagatgt cctgctggat
    10441 atcaacaaat ggggtatcga ccataccttt gaatgcatcg gtaacgtcaa cgtgatgcgt
    10501 gcggcgctgg aaagtgcgca ccgcggctgg ggtcagtcgg tgatcatcgg ggtagcaggt
    10561 gccggtcagg aaatctccac ccgaccattc cagttggtca ccggtcgcgt atggaaaggt
    10621 tccgcgtttg gcggcgtgaa aggtcgttcc cagttaccgg gcatggttga agatgcgatg
    10681 aaaggtgata tcgatctgga accgtttgtc acgcatacca tgagcctgga tgaaattaat
    10741 gacgccttcg atctgatgca tgaaggcaaa tccattcgaa ccgtaattcg ttactgattt
    10801 cccgcaggta tacccgtcca cttcagacgg ggttcttaat actctccccg ggcagccgtc
    10861 cgggggatta accgtgagat aatgactgat ggaactcatt gaaaaacatg ccagctttgg
    10921 cggctggcaa aatgtgtatc ggcattattc ccaatcactg aaatgtgaaa tgaatgtcgg
    10981 cgtctatctc ccaccaaaag ccgcgaatga aaaattgccg gtgctgtact ggctttcagg
    11041 cctgacctgc aacgagcaga atttcattac taaatcgggg atgcagcgtt acgcggctga
    11101 gcacaacatt attgttgttg cgccggacac cagtccgcga ggcagtcatg tcgcagatgc
    11161 tgaccgttac gatctcgggc aaggtgccgg gttttacctg aacgcgacgc aagcgccgtg
    11221 gaatgaacat tacaaaatgt atgactatat ccgcaacgag ctgccggatt tagtgatgca
    11281 tcattttccg gcaacggcca aaaagtctat ctctggtcat tctatgggcg ggctgggcgc
    11341 gctggtgctg gcgttacgta acccagatga atatgtcagc gtctcggcgt tttcgcccat
    11401 tgtctcccca tcgcaagtgc cgtggggaca gcaagccttt gctgcatatc ttgctgaaaa
    11461 taaagatgcc tggttggatt acgacccggt gagtcttatt tcacaaggtc aacgcgttgc
    11521 ggaaatcatg gttgatcagg ggttgagtga tgatttttac gcagaacagc tgcggactcc
    11581 aaatcttgaa aagatctgcc aggagatgaa tatcaagacg ttaatccgtt atcacgaggg
    11641 ttatgatcac agctattatt ttgtctccag ttttattggc gagcatattg cctaccacgc
    11701 caataaactg aatatgcgtt gataatagtg cacgactgcc ggatgcggcg tgaacgcctt
    11761 atccggccta cacttcgccc gtaaaccgta ggcctgataa gacgcgcaaa gcgtcgcatc
    11821 aggcatggcg cacgactgcc ggatgcggcg tgaacgcctt atccggtcta cacttcgccc
    11881 ataaaccgta ggcctgataa gacgcgcata gcgtcgcatc aggcatctgc gcacgactta
    11941 tctatgttta ccacatcaaa tcatcaggca ctttgaagtc ggcatacgga tcgtcttcat
    12001 cctgctcttc ggcactgagc gcgctgtgca acacaatact gctggcatca cgctgagcaa
    12061 ttttatcggc aacactggcg ggaataatgg tgtattcacc ttcgctattg ttatcgacca
    12121 acaagcgggc aatcgccaga cgaccattaa tcaactgcgc ctgtgtgagc ttatcgacaa
    12181 aaatcttctt aatcagattg ccgtcagtga agttaaaacc aatatcgcca ttggcaatgg
    12241 tgattcggtt catttcaatg agctgcttca cctgggcttt atattctttc gccaacgccg
    12301 cttgtttttg ctgttcgcta agctgtttat cacgctcaag ctgtgccttt ttattttctt
    12361 ctaccgccgc ccgagcttca cgcgcctgaa cgcgcgattt tttcgccgtt ctctccacct
    12421 tcgccgcttt tttgctggtg actaatcctg ctttgagcaa ctgctcttgt aaggtaagtt
    12481 ttgccatctt tgtttccaaa cccgctgaat aatttttagc gagtatacct gtaaacgccg
    12541 tgggtgtatt gggggcaagt ccctttcgcc cctgcttaat aaggcgcgtc aggcatcggc
    12601 acacggctgc attcttgatc tccgactcat caaaataaac accgccaacc cagcaacaag
    12661 aatacccggt gcagaagcgg ccattacgcc gaccgtgcca gtgcctaatg ccagcatttt
    12721 cccggccagt aacggaccgc tcatagcccc cagacgccct accgccacgg ctgttcccac
    12781 acctgttgcg cggatctgcg aactgtaaaa caacggtgcc agggcataca aaacgctttg
    12841 cccacctgtc gcaaacaacc ccgcgacaaa tcccgccagc aacataccgt taaatgacga
    12901 caccgttcca agcgccagca gcgaagctaa catgccacta taaatcagta gcgacatggt
    12961 tactggacgc agcttatcca tcaatgcgcc caacattaac gtcccgcttg ccgcccccat
    13021 ttgcagggca aacatcaccc ctgccgcctg cgatggctgg aatccttgct ccaccaaaag
    13081 tagcggtagc cagttgatca acatataaac caccagcaga gtgaagaaat aacacaacca
    13141 cagcagcagc gtcgcggttg ccgtttctgg cgcaaataag gcacgcagtg gtggcgcagc
    13201 ctgtttttcg ccagcgaaaa ccgctgactc cggcagccag cgcattaata gcggcaccag
    13261 aatcaacggc accacaccac ctacccaaaa caccgtttgc catgctaagt ttgcccccgc
    13321 gaaacccagt gtcgccgcca gcgccgcgcc aatgggaaca ccgcaataca tcaggctcac
    13381 tgccgtccca cgaaaacgtg gacccgcggc ttcagacgtc agggcgataa gattcggcaa
    13441 cgccgccccc agcccgacac cggtcatcag ccgcgcaaag accagtgagg ggaaatccca
    13501 ggcaatcgcc gttgccagtg agaacaaacc aaacagcgca actgagccaa tcaaaatacg
    13561 cttacgacca taacggtctg ccagcatccc gccaaccaac gcgccgggta gcaaaccgag
    13621 tattccggcg ctaaatatcc agcccatttg cattttatcg agtgcgaaag cctgggcgat
    13681 gccacccgcc gcaatgccag ccgcctgaag atccagccct tccatcagag cgaccaaaaa
    13741 acaaagcccg atggtcagca tcaggcggga tgaagatgat gaaggggtac gagtcgacat
    13801 aagatgaacc tcgttgcgag tgcaggtgaa tcgcgactga tacattgcca gccgcgtaat
    13861 cagaactatt taatgactgc ctacgggcga aatgtaggcc ggacaagacg tttatcgcat
    13921 ccggcagtcg tg
//
LOCUS       DACAPB010000124        13924 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.124, whole genome shotgun sequence.
ACCESSION   DACAPB010000124 DACAPB010000000
VERSION     DACAPB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.124"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..193)
                     /locus_tag="IT904_003201"
     CDS             complement(<1..193)
                     /locus_tag="IT904_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3192944.1"
                     /translation="MILWPMKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAERVEMD"
     gene            201..530
                     /locus_tag="IT904_003202"
     CDS             201..530
                     /locus_tag="IT904_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3192945.1"
                     /translation="MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            complement(412..909)
                     /locus_tag="IT904_003203"
     CDS             complement(412..909)
                     /locus_tag="IT904_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192946.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(1018..1251)
                     /gene="yedF"
                     /locus_tag="IT904_003204"
     CDS             complement(1018..1251)
                     /gene="yedF"
                     /locus_tag="IT904_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAP3192947.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1248..2453)
                     /gene="yedE"
                     /locus_tag="IT904_003205"
     CDS             complement(1248..2453)
                     /gene="yedE"
                     /locus_tag="IT904_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAP3192948.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGLLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2640..3053
                     /locus_tag="IT904_003206"
     CDS             2640..3053
                     /locus_tag="IT904_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP3192949.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3087..4574)
                     /gene="amyA"
                     /locus_tag="IT904_003207"
     CDS             complement(3087..4574)
                     /gene="amyA"
                     /locus_tag="IT904_003207"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP3192950.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4652..5017)
                     /gene="fliT"
                     /locus_tag="IT904_003208"
     CDS             complement(4652..5017)
                     /gene="fliT"
                     /locus_tag="IT904_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAP3192951.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5017..5427)
                     /gene="fliS"
                     /locus_tag="IT904_003209"
     CDS             complement(5017..5427)
                     /gene="fliS"
                     /locus_tag="IT904_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP3192952.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5443..6858)
                     /gene="fliD"
                     /locus_tag="IT904_003210"
     CDS             complement(5443..6858)
                     /gene="fliD"
                     /locus_tag="IT904_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP3192953.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     TQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SNNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            7106..8155
                     /locus_tag="IT904_003211"
     CDS             7106..8155
                     /locus_tag="IT904_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAP3192954.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVAITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            8319..9038
                     /locus_tag="IT904_003212"
     CDS             8319..9038
                     /locus_tag="IT904_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAP3192955.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            9084..9635
                     /gene="fliZ"
                     /locus_tag="IT904_003213"
     CDS             9084..9635
                     /gene="fliZ"
                     /locus_tag="IT904_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAP3192956.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTYCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            9723..10523
                     /gene="tcyJ"
                     /locus_tag="IT904_003214"
     CDS             9723..10523
                     /gene="tcyJ"
                     /locus_tag="IT904_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3192957.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTLKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            10628..11614
                     /gene="dcyD"
                     /locus_tag="IT904_003215"
     CDS             10628..11614
                     /gene="dcyD"
                     /locus_tag="IT904_003215"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAP3192958.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLLPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            11629..12297
                     /gene="tcyL"
                     /locus_tag="IT904_003216"
     CDS             11629..12297
                     /gene="tcyL"
                     /locus_tag="IT904_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAP3192959.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGVILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            12294..13046
                     /gene="yecC"
                     /locus_tag="IT904_003217"
     CDS             12294..13046
                     /gene="yecC"
                     /locus_tag="IT904_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAP3192960.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            13276..>13924
                     /gene="sdiA"
                     /locus_tag="IT904_003218"
     CDS             13276..>13924
                     /gene="sdiA"
                     /locus_tag="IT904_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAP3192961.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQK"
BASE COUNT         3464 a         3446 c         3588 g         3426 t
ORIGIN      
        1 catccatttc aacccgttca gcttcaaaat cagcacatac atttgaaaag taagtgcgta
       61 gtttttctgt cgcgtcatgg tcaaaaatct gacgtcggta tttagtaacg aagactaggt
      121 gaacatgctt caggaaaaca caatgccttc cgcgtcggat atcggtttct tttttcatag
      181 gccaaagtat gatttggccc atgaaacgac tacaggcatt caaattccgg ttaagaccag
      241 gtggtcaaca ggagcgtgaa atgaggcgct tcgcaggcgc atgtcgtttc gttttcaatc
      301 gtgctctggc gcttcagaat gagaatcatg aggccggtaa taaatacatc ccttacggga
      361 aaatggcttc ctggttggtt gagtggaaaa atgccactga aacgcaatgg cttaaagatt
      421 cgccctcaca gccattgcaa cagtcactga aagaccctga gcgggcttac aaaaacttct
      481 tccggcttcg tcaccacgct caaacggtgt gctacctatc gcggctataa aaacagcttg
      541 tccatcagca cgatagataa aataaggctg ctttttgtcg ccttcttttt tccactcaaa
      601 ccagccatcg gcaaaacaga ttgcccgacc atgttgccag agcggtttaa acatgtgact
      661 ggttgcggca gtttctacgc gggcgttaat cagcggcggt ttatcccacc atccgggagc
      721 atatccccag aaaaccggat ccagatgaag gtgttcatca cgttcactga gcagaaggac
      781 tttggttccc ggcgcgacgt tgtatctgcc aataggttcg ggatcgtagg gaatatcgcg
      841 ttcaatatct tccgcgagaa gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg
      901 tccacacata gaaacctcct gaggtcacac tgaaaagaat aggagagggg gcgggttaaa
      961 tctatgtgat agagaggcaa cgatattccc gctgtgggcc acagcgggaa tgagggatta
     1021 cttctgaatt aaataacgaa tggtcggccc gtcttgctga atatccagca ccgtatagcc
     1081 gtgattacgc gcatccagtg gaatattatt gatcgactgc ggacagtcgc tcaccacttc
     1141 caggatctct ccttttttta actgcggcat cgcctcaagg gttgcgactg ccggataagg
     1201 gcagggttca cccaccatat caagacggta atcaggaacg atatttttca tgcgatctcc
     1261 ttagcagtct gcggtgccgc acggcggaag aagcgttttt cccagccaat aatcaacatt
     1321 agagcagcaa acaacagcaa atatgtcacc agcaggccgc ccatcgggcc aaaggttttc
     1381 agcaggttga ttttgtccca gtcggtggcc agcgccggag cgaaatcatc ccagtagtac
     1441 gccagaatcg ttgagccgat cacgttgccc agaccgaccc accagtagtg cacctggcct
     1501 tctaccgcgc ggtacatcca gccggtttcg cagccgcctg ccagcacgat gccaaaacca
     1561 aacagtaaac caccaattac cgcgtttggt cccgcccaca tgattttggg ttcaacgcct
     1621 aactgtacgt aactgaagat cccgatggca cttaccgcca taccgataat gattgctttc
     1681 gccatatggg tacgtccggt gatccacatg tcgcggaacg ccgaagtaaa gcagatttgc
     1741 gcacgttcaa tcagtaaacc aaagccgacg ccaaacagca ttgccagccc cagtttgggc
     1801 tggttcatcg ccgtcagcag cgcccagccc agcaggccga aaaagaccag catcccgaga
     1861 cgaaaacgac gccgcgcctg atccggtttt tgcgtcaacg gtgaggcagc agaaactttc
     1921 tgcattttca cgggaatacg gaagatgggc agaagggtaa agcgcgcgcc aaaccatgaa
     1981 ccaatggcag tggcgatggc aaagaaccag gcgtgcagcg agaactgtgg aattccggta
     2041 aagaacgccg ccaggttaca gcccattgcc agacgtgcgc caaaaccggc gataataccg
     2101 ccaatgatgg cctgcataat gcggatacgg ctgcgcggca tgcgcagttt gacattgttg
     2161 gcccacagcg ctgcggcaaa acagccgcca aacataccga ggatcatcat tccgtcgatg
     2221 cgggttaatg gcgatccttc cagatggata attttaaaat aaccccactc ttcagcatgg
     2281 acgccgaaca actgcaggag ctgaccgccc caacgggtaa attcacctgt gacagcccaa
     2341 aaggtgccag tgatgccaaa atagtaagtg gagagaatac ccgccgcgat gaccgcgggg
     2401 atgggcgccc agaatttaat caaccaggcg tgtttgaatt gctgccatga catgaatgta
     2461 gcctctgaac tcagaaggtt tgaaacaaga gtcgcgaatc atacaccatg caaatttatt
     2521 ttcggggtaa taattcacaa aaataatggt tagatcaaat ttctctctga acctggaaag
     2581 gctggcgaca tcagggcttt aatgccacaa tcctcttttc ttctcatgca ggacgaatga
     2641 tgaaaaaact cgcaattgca ggtgcgctca tgttgctggc gggctgtgcc gaagtcgaaa
     2701 actataacaa tgtcgtgaaa acccccgcgc cggactggct ggcgggctac tggcagacca
     2761 aagggccgca acgtgcgttg gtcagtccgg aagcgatagg cagcctcatt gtcactaaag
     2821 agggcgatac cttggattgc cgtcagtggc agcgggtgat tgccgtgccg ggtaagttga
     2881 cattgatgtc tgatgatctc actaacgtta ccgtaaaacg tgagctgtac gaggttgagc
     2941 gtgacggtaa caccattgag tacgatggta tgacgatgga gcgtgtggat cgcccgacgg
     3001 cagagtgtgc cgccgcgctg gataaagcgc cgttaccgac gccactgccg taatgaaatc
     3061 tccccggctt gccgccgggg atggatttaa atcacctctt cgataaccca cacgctgacg
     3121 ctgccgccgt tgcaaaagaa ggtcgcttcg ccgttttcgt ctgtcactac gctctcttgc
     3181 cggttcccca gaaaatcacg ccaggtttta ttgccgtaat tctctcccag atgaatggtt
     3241 ttttcgccat catctccgtt cgacatgacc accacgcagc cgggatattc gtcggtgcca
     3301 ctgcggctaa aggcaatgca gttcggatgg tcgaaaaata acgtctgtac accgtgggcg
     3361 aaacgctgac gggcgagaat taactcatca agctgttcga ttattggcat atctatcgga
     3421 taggtttgcc cgtcaccacc gacatcttcg taatgtgcac cgtagaggtc cggatagaac
     3481 accgaaggaa cgccattttc ccgcaacaaa attaaggcat acgccagcgg tttaaaccac
     3541 ggttcgacgg gggcttcgag ggcttgcaac ggttgggtgt cgtggttggc aacgagggtt
     3601 acggcgtgaa aaggatcggc ttccaccagc gtcccagtga agatctgcgt catgtcgtag
     3661 tcgcgaccca tgcgcgatgc ttcatggaat ttcatctgca gcggcgcatc aaacagcatg
     3721 gttttgcctt ccacctgatc aatatacgtt tgcagtttat cgacttcatg cgaccagtat
     3781 tcagccacaa taaacaacgg ctttggcgca acttcctgta cgtgttcgat ccactcttta
     3841 taaaaccatg ccggaatatg tttgaccgca tcaagacgaa agccgtcgca ttgcgtttgt
     3901 tccatcaccc agcgcgccca gtatttaatc tcttccgtca cggcatgatt gcgaaaatcg
     3961 atattctccc ccatcagata atcgaaatta ccgagttcat catcaacctg atcgttccag
     4021 ccttctccgg tatagtcgtt aacaatttta aaaatgccat cttcatcagg gttttcgata
     4081 tggtcgatac cgctaaaaca tttgaaatcc cagataaact gcgagtattg cccggcacga
     4141 gcggggaagg tgtaacgcgt ccagccttca cactcaatga tttcttcgtc aatttgcgta
     4201 cggtcatcag catttacacg ctgcacgcga atagcttctt tttcatccgc gcccattttg
     4261 tggttgacta ccacgtccag cagcaccgca atgtcattac gtttcagggc gtcaatggcg
     4321 gccagcagtt gtgctttatc accatattta gtagggatgc tgcctttctg atcaaactca
     4381 cctagatcaa ataaatcata ggagtcgtag ccgaccgaat agccgcccga tgcgccttta
     4441 taggcgggcg gcaaccagac catattgata ccaatatcat taaaaccgtc tgcgcgctcg
     4501 gccagttcag gccagagctt accgccttcc gggtaatacc agtgaaaaca ttgtaacagc
     4561 gtgggattac gcattcttcg actccattca aggggaatat tagaagcgta gccgtaatcg
     4621 gattattcgc gagccatcgg ctcattaaga ttcaaaagag gttatcctgc ggagccagca
     4681 caaagccgcc ctgatcgcca taggcactta acaccgattt ttgcaccgat gactgaccga
     4741 ccagtttcgc cagttcatcc atccgaatct gtaataactg ttttaccttg ctttcgttgt
     4801 cgagaatcag gcgcagcatc gggcggagct gctcctgcat cgtagtggac ggggcaacct
     4861 cttcagtcag gtgtgcgatc tcctgcaccg cattcacata cgccatttcg ctggcgatga
     4921 gttcgtccca ttgttcttcc gttgccaggc gtaacattag ctggcttttt tcgacgagtt
     4981 gttgccaggc gaaatacaaa tgcggtgcat tgttcatcag actgggtcct gaatcaaaga
     5041 aggggagagt aaagactctt tccaggcatc ggcaatattg cgcattaatg cttccacttc
     5101 ttcgactgcg gagacatcgt tacgtaaatt ggcttgcagc aagcgcctga ccatatagct
     5161 ataaagagca atcaagtttt gggttaattc gtctttgctc tcttcatcaa gactcacccg
     5221 cagtccgttc tcaatgatgt tgatcgcttt tgataaagag acgcctttgc cttgctgatt
     5281 gttgtcttgc ataaacaggc gcgctctaac cagtgcgctc agcactccat caaatagcat
     5341 ggtgaccagc tgctgttggc tggcgctcat tacggcgctt tcgacgccaa tttgtgcata
     5401 ggcctgggtg ccttttgccg cgtacatagt ctctcctgaa gattatttcg agttgctatt
     5461 tgaggtgtcg aactgctgtg tcagatagct actagtggag ttcagcgagt tcattaacac
     5521 atctaattgg gtgaactggg ctttatagcg cgtcattaag gtatcgatac gttcactggc
     5581 tgcgttgtac tggtcggtca aattattcag cgttttgctg acgccatctt ttgctgcctg
     5641 gattatcccg gtggaagaga gccaactggt caggttgtta ctaatgccgg tggttacccc
     5701 agtacttttg ccgtcaccta taaacatttc accgatacca gcggcatcat tttttagcgc
     5761 cgtttgcagt ttgtcggtcg cgatttccag cttgccggta ctggcatcgc tggtaatgcc
     5821 aatctgtgcc agcgttttat agtttgaact gctgtgagta ttggcgagca aggttttaag
     5881 ctgagtctgg atggtacgca gtgtggagtc accgagtaat gcgccattac tggagtcctg
     5941 actttcagct ccggcatcga cagcggtgta tttggttaag ctgctgaatg tatcctgcaa
     6001 tgtgttatag gcatccaccc acgctttaac cgcattttca gctttggatg tgtctttact
     6061 gatggtcagt gtctgattac cggtggtgac gtcattcagg ttgagggtga tatcttccag
     6121 tgagtcgctg atagtgttac tgctgttttc aatctcaacg ttattgactg tcagtttggc
     6181 gttttgcgcg gtgacgcttt caatcatgcc attgctgcta tcacccggcg ttccgttgta
     6241 acccataaaa ctttccaggg cgctgtcgcc gctgacgcta agtttcatag cgttattgct
     6301 gcccgtatcg ttagcggtga tggacagacg atattcgccg tttcccacat tgatgatact
     6361 ggcgctgaca ccagctttgg cgttgttaat ggcatcgcga atgccggaaa gtgacgagtt
     6421 acccgcgctg atatcaatcg tgaccggatc tttaccgccg ccttgttgga tggtcagtac
     6481 gctatcgctg gtggcgatgg cggctttact gtctttttga ctattttttg ttgtcagcgt
     6541 ctgtgcctgc gtcagttggc taacgctaat ggtgtatttc ccagcaatcg cgctgcccgt
     6601 ggtggtggcg ctgaaagccg atgaactgct ggtggttgag gtggcggtaa ataaatcggc
     6661 tttattcagt gctgtgttgg ctgtctggaa actctctagt gcacttttca gcgtcccgta
     6721 ggcgctcagt ttcgcggtgt aagagctttg ctgttttgat atcggcgtca gggtagattt
     6781 ttctgccgcc tccagactgt caaggatgct gcttaagtcc agccccgacc caaccccaag
     6841 cgttgaaata ctagccatat agtttcctta atcatgcgac gtgtatagaa cgaataccgg
     6901 ggttatcggc gtaagcgggg taaagtttac gatttatttt ttgccttaat ggcacgaaca
     6961 gcaacgagga aggggagtat ttcgaccgct agaaaaaaat tctaaaggtt gtgagtgacc
     7021 agacgataac agggttgacg gcgacgaagc cgaagggtgg aagcccaata cttaaaccgt
     7081 agacttgaaa acaggaaaat gaattatggc acaagtcatt aataccaaca gcctctcgct
     7141 gatcactcag aacaacatca acaaaaacca gtctgcgctg tcgacttcta tcgagcgcct
     7201 ctcttctggt ctgcgtatta acagcgctaa agatgacgcc gcgggccagg cgattgctaa
     7261 ccgctttact tctaacatca aaggtctgac tcaggccgca cgtaacgcca acgacggtat
     7321 ttctctggcg cagacggctg aaggcgcgct gtcagagatt aacaacaact tgcagcgtat
     7381 tcgtgaactg accgttcagg cctctaccgg cacgaactct gattccgacc tgtcttctat
     7441 tcaggacgaa atcaaatccc gtcttgatga aattgaccgt gtatctggtc agacccagtt
     7501 caacggtgtg aacgtgctgt cgaaaaacga ttcgatgaag attcagattg gtgccaatga
     7561 taaccagacg atcagcattg gcttgcaaca aatcgacagt accactttga atctgaaagg
     7621 atttaccgtg tccggcatgg cggatttcag cgcggcgaaa ctgacggctg ctgatggtac
     7681 agcaattgct gctgcggatg tcaaggatgc tgggggtaaa caagtcaatt tactgtctta
     7741 cactgacacc gcgtctaaca gtactaaata tgcggtcgtt gattctgcaa ccggtaaata
     7801 catggaagcc actgtagcca ttaccggtac ggcggcggcg gtaactgttg gtgcagcgga
     7861 agtggcggga gccgctacag ccgatccgtt aaaagcactg gatgccgcaa tcgctaaagt
     7921 cgacaaattc cgctcctccc tcggtgccgt tcaaaaccgt ctggattctg cggtcaccaa
     7981 cctgaacaac accaccacca acctgtctga agcgcagtcc cgtattcagg acgccgacta
     8041 tgcgaccgaa gtgtccaaca tgtcgaaagc gcagattatc cagcaggcgg gcaactccgt
     8101 gctgtctaaa gccaaccagg taccgcagca agttctgtct ctgttacaag gctaatggcc
     8161 ttaacctgcc tgaccccgct accggcgggg ttttttctgc ccgcaattta ccggtaaccc
     8221 ccaaataacc cctcatttca cccactaatc gtccgattaa aaaccctgca gaaacggata
     8281 atcatgccga taactcatat aacgcagggc tgtttatcgt gaattcactc tataccgctg
     8341 aaggtgtaat ggataaacac tcgctgtggc agcgttatgt cccgctggtg cgtcacgaag
     8401 cattgcgcct gcaggttcga ctgcccgcga gcgtggaact tgacgatctg ctacaggcgg
     8461 gcggcattgg gttacttaat gccgtcgaac gctatgacgc cctacaagga acggcattta
     8521 caacttacgc agtgcagcgt atccgtggcg ctatgctgga tgaacttcgc agccgtgact
     8581 gggtgccgcg cagcgtgcga cgcaacgcgc gtgaagtggc acaggcaata gggcaactgg
     8641 agcaggaact tggccgcaac gccacggaaa ctgaggttgc ggaacgttta gggatcgata
     8701 ttgccgatta tcgccaaatg ttgctcgaca ccaataacag ccagctcttc tcctacgatg
     8761 agtggcgcga agagcacggc gatagcatcg aactggttac tgatgatcat cagcgagaaa
     8821 acccgctaca acaactgctg gacagtaatc tgcgccagcg ggtgatggaa gctatcgaaa
     8881 cgttgccgga gcgcgaaaaa ctggtattaa ccctctatta ccaggaagag ctgaatctca
     8941 aagagattgg cgcggtgctg gaggtcgggg aatcgcgggt cagtcagtta cacagccagg
     9001 ccattaaacg gttacgcact aaactgggta agttataacg tcagtaaatg ccgcacttta
     9061 attttgacta ccaggagttc ttaatgatgg tgcagcacct gaaaagacgg ccattaagcc
     9121 gctatcttaa agactttaaa cacagccaga cttattgcgc gcattgccgt aaattactcg
     9181 atcgcattac cctcgttcgc gacggcaaaa tagtgaataa aatcgagatt tcccgcctgg
     9241 acgcgctgct tgatgaaaat ggctggcaaa cggaacaaaa atcatgggcg gcattgtgcc
     9301 gattttgcgg tgatttacat tgcaaaacgc agagtgattt tttcgatatt atcggcttta
     9361 agcaatttct ttttgagcaa actgaaatga gcccaggtac ggtgcgtgaa tatgtcgttc
     9421 gtttgcgccg tttggggaat catctacacg agcaaaatat ttccctcgat cagctgcagg
     9481 acggtttcct tgatgaaatc ctcgccccgt ggctgcccac caccagcacc aacaattacc
     9541 gcatcgcatt acggaagtat caacactatc agcgtcaaac gtgtaccgga cttgtgcaga
     9601 aatcctccag cctgcctgct tctgatatat attaaataag aataagatgt agcggagttg
     9661 tttttgtgtt tacaaacaat ggctctacac tgcaaacaga cataacaaca ttcggggtaa
     9721 atatgaaatt agcacatctg ggacgtcagg cattgatgag tgtgatggcc gtagcgctgg
     9781 ttgcgggcat gagcgttaaa agttttgcag atgaaggtct gcttaataaa gttaaagagc
     9841 gcggcacgct gctggtaggg ctggaaggca cttatccgcc gttcagtttt cagggggatg
     9901 acggcaaatt aaccggtttt gaagtggaat ttgcccaaca gctggcaaaa catcttggcg
     9961 ttgaggcgtc actaaaaccg accaaatggg acggtatgct ggcgtcgctg gactctaaac
    10021 gcattgatgt ggtgattaat caggtcacca tttctgacga gcgcaagaaa aaatacgatt
    10081 tctctacccc ttacaccatt tccggtattc aggcgctggt gaaaaaaggt aacgaaggca
    10141 cccttaaaac ggctgctgat ctgaaaggca aaaaagtggg tgtcggtctg ggcaccaact
    10201 atgaagagtg gctgcggcag aatgttcagg gcgtcgatgt gcgtacctat gatgatgacc
    10261 cgaccaaata tcaggatctg cgcgtagggc gtatcgatgc gatcctcgtt gatcgtctgg
    10321 cggcgctgga tctggtgaag aaaaccaacg atacgctggc agtaaccggt gaagcattct
    10381 cccgtcagga gtctggcgtg gcgctgcgta aaggaaatga ggacctgctg aaagcggtga
    10441 atgatgcaat tgcggaaatg caaaaagacg gcactctgca agctctttcc gaaaaatggt
    10501 ttggtgctga tgtgaccaaa taatcagcat aatgacaaaa aagggcgctt tcactagcgc
    10561 cttttttatt tacgcgtttt cagcgtgcat aataagaaaa tcacaacaac acacatcgga
    10621 ggctgttatg ccactgcata atttaacccg ttttccacgg ttggagttta tcggcgcgcc
    10681 aacgccgctc gaatatctgc cgcgcttttc tgattatctt ggacgggaaa ttttcatcaa
    10741 acgggatgac gtcaccccca tggcaatggg cggcaataaa ttacgtaagc tggaatttct
    10801 cgcagcagat gctctgcgcg aaggtgccga tacgctgatt actgccggcg cgatccagtc
    10861 taaccatgtg cgccagactg ccgcagttgc ggcgaaactc ggtctgcact gcgtggcgct
    10921 gctggaaaat cctattggca caaccgcaga aaactattta accaacggca atcgcttgtt
    10981 gctggatctg ttcaataccc agattgaaat gtgcgacgca ctgaccgatc ccaatgccca
    11041 actggaagag ctggcgacgc gagtcgaagc acaaggcttt cgcccgtatg tcattccggt
    11101 tggcggttct aatgctttgg gcgcgctggg ttatgtggag agtgcgctgg aaatcgcgca
    11161 acagtgtgaa ggggcggtta atatttcgtc ggtggtagtc gcatcgggca gtgccggaac
    11221 tcacgccgga ctggctgttg ggctggaaca cctgctgcct gaaagcgaac tgattggcgt
    11281 gaccgtgtcg cgttccgttg ccgatcaatt gccgaaagtg gttaacctac aacaggcgat
    11341 tgcgaaagaa ctggagctga ccgcatcagc ggaaatttta ctctgggatg actattttgc
    11401 acctggctac ggcgtgccga acgacgaagg catggaagca gtgaaattgc tggcgcggtt
    11461 ggaaggcatt ctgcttgatc ctgtgtatac cggaaaagcg atggcgggtc tgattgacgg
    11521 tatcagtcag aaacgcttca aagatgaagg gccgattctg tttattcata ccggcggcgc
    11581 gcctgcgctg ttcgcctatc atccccacgt ttagctccgg gaacgctcat gcaagaaagt
    11641 atacaactgg ttattgattc cttgccgttc ctgctgaaag gggctgggta tacgctgcaa
    11701 ctcagtatcg gcggcatgtt ttttggctta ctgctcgggg ttattctcgc gctgatgcgc
    11761 ctgtcgccaa tctggcccgt gcgctggctg gcgcgttttt atatctctat ttttcgtggt
    11821 acgccgctca tcgcacaact gtttatgatc tattacggcc tgccgcagtt tggtatcgaa
    11881 ttagatccca ttccgtcagc aatgattggt ctgtcgctga acactgccgc ctatgctgcc
    11941 gaaacgctgc gcgccgccat ttcttctatt gataaaggtc agtgggaagc agctgccagt
    12001 attggtatga cgccatggca gactatgcgt cgcgcgattt tgccacaggc ggcgcgggtg
    12061 gcgttgccgc cgctgtcgaa cagctttatc agtctggtaa aagatacgtc gctggccgcg
    12121 acaatccagg tgccggagct gttccgtcag gcgcagttga ttacctcgcg tacgctggag
    12181 gtattcacca tgtatctggc ggcttcgctc atctactgga tcatggcgac tgtgttatcg
    12241 acgttgcaga accattttga gaatcaactt aatcgccagg agagagaacc aaaatgagtg
    12301 ccattgaagt taagaacctg gtgaaaaaat ttcacggtca gacggtgctg cacggtatcg
    12361 accttgaggt aaagcctggc gaagtggtgg caattatcgg tccgagtggt tccggcaaaa
    12421 ccacgttgct acgcagcata aatctgctgg aacaacccga agcgggaacg atcaccgttg
    12481 gcgatatcac tattgatact gcacgttcat taagtcagca aaaatcgctg attcgccagt
    12541 tgcgtcagca cgtcgggttt gtcttccaga actttaattt gtttccgcat cgtacggtgc
    12601 tggaaaacat tattgaaggg ccggtgatcg tcaaaggcga accgaaagaa gaggccacgg
    12661 cgcgcgctcg cgagctgttg gcgaaagttg ggctggccgg taaagaaacc agctatccac
    12721 gtcgtttgtc tggcggtcaa cagcaacgtg ttgcgattgc acgtgcgctg gcaatgcgtc
    12781 ctgaggtgat tttgtttgac gagccaacgt cggcgctgga tccagagctg gtgggcgaag
    12841 tcctgaacac catccgtcag ctggcgcagg aaaagcgcac gatggtgatt gtgacgcacg
    12901 aaatgagctt tgcccgggat gttgcggacc gggcgatttt tatggaccag gggcggatag
    12961 tcgagcaggg ggcagcaaaa gcgttatttg ccgatcccca gcagcctcgc acccgccagt
    13021 tcctcgagaa gtttctgctg caataataag aaaaaatcag ccccgacgat tcacctgtcg
    13081 gggcgggacg ccatttcaag cgtgataaaa cagcttaaca aatcagcata actcattaat
    13141 aacataagag aatgcgatgg cttgcaaaag taattcattg cctgaataat ataaattata
    13201 tataaatctt atttatgtga tagtttgaat tatcattata aatgatactc actctcaggg
    13261 gcgttgcggt ttactatgca ggatacggat tttttcagct ggcgtcgcac gatgctgttg
    13321 cgttttcaaa ggatggaggc cgcagaagag gtctaccatg aaatagagct tcaggctcag
    13381 cagctagagt acgattacta ttcgttatgt gttcgccacc cggtaccatt cacgcgacct
    13441 aaagtggctt tctacaccaa ttaccctgag gcgtgggtta gttattatca ggcaaaaaac
    13501 tttctcgcaa ttgatccggt gctgaaccct gaaaacttta gtcagggcca tttaatgtgg
    13561 aatgatgact tattcagcga agcacagccg ttatgggaag ccgcgcgcgc acatggttta
    13621 cgccgcggtg tcactcagta tttaatgctg ccaaaccggg cgctgggctt tttgtccttt
    13681 tcccgtagca gcgcgcgcga aatacccatt cttagtgatg aactgcaatt aaaaatgcag
    13741 ttactggtgc gcgaaagtct gatggctctg atgcgtttaa atgatgaaat agtgatgacg
    13801 ccagagatga atttcagcaa gcgcgaaaaa gaaattttga aatggacggc ggaagggaag
    13861 acatcagcag agatagcgat gattttgtca atctctgaga atacggtcaa tttccatcag
    13921 aaaa
//
LOCUS       DACAPB010000125        13853 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.125, whole genome shotgun sequence.
ACCESSION   DACAPB010000125 DACAPB010000000
VERSION     DACAPB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.125"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(43..419)
                     /gene="rnpB"
                     /locus_tag="IT904_003219"
     ncRNA           complement(43..419)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IT904_003219"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(452..1597)
                     /gene="garK"
                     /locus_tag="IT904_003220"
     CDS             complement(452..1597)
                     /gene="garK"
                     /locus_tag="IT904_003220"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAP3192962.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNVG"
     gene            complement(1694..2584)
                     /gene="garR"
                     /locus_tag="IT904_003221"
     CDS             complement(1694..2584)
                     /gene="garR"
                     /locus_tag="IT904_003221"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP3192963.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2614..3384)
                     /gene="garL"
                     /locus_tag="IT904_003222"
                     /pseudo
     CDS             complement(2614..3384)
                     /gene="garL"
                     /locus_tag="IT904_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3400..4734)
                     /locus_tag="IT904_003223"
     CDS             complement(3400..4734)
                     /locus_tag="IT904_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3192964.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPAMCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5109..6680
                     /gene="garD"
                     /locus_tag="IT904_003224"
     CDS             5109..6680
                     /gene="garD"
                     /locus_tag="IT904_003224"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAP3192965.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     RLHNQLAVFNPAPVT"
     gene            6829..7164
                     /gene="prlF"
                     /locus_tag="IT904_003225"
     CDS             6829..7164
                     /gene="prlF"
                     /locus_tag="IT904_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HAP3192966.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7164..7628
                     /locus_tag="IT904_003226"
     CDS             7164..7628
                     /locus_tag="IT904_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HAP3192967.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7683..8492)
                     /gene="agaR"
                     /locus_tag="IT904_003227"
     CDS             complement(7683..8492)
                     /gene="agaR"
                     /locus_tag="IT904_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAP3192968.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8741..10021
                     /gene="kbaZ"
                     /locus_tag="IT904_003228"
     CDS             8741..10021
                     /gene="kbaZ"
                     /locus_tag="IT904_003228"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAP3192969.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10044..10517
                     /gene="agaV"
                     /locus_tag="IT904_003229"
     CDS             10044..10517
                     /gene="agaV"
                     /locus_tag="IT904_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP3192970.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10528..11307
                     /locus_tag="IT904_003230"
     CDS             10528..11307
                     /locus_tag="IT904_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAP3192971.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11297..12175
                     /locus_tag="IT904_003231"
     CDS             11297..12175
                     /locus_tag="IT904_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP3192972.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQNIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12193..12627
                     /locus_tag="IT904_003232"
     CDS             12193..12627
                     /locus_tag="IT904_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP3192973.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12624..13757
                     /gene="nagA"
                     /locus_tag="IT904_003233"
     CDS             12624..13757
                     /gene="nagA"
                     /locus_tag="IT904_003233"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP3192974.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
BASE COUNT         3318 a         3669 c         3585 g         3281 t
ORIGIN      
        1 tccaaaagca aaaacccgcc gaagcgggtt tttacgtaaa tcaggtgaaa ctgaccgata
       61 agccgggttc tgtcgtggac agtcattcat ctaggccagc aatcgctcac tggctcaagc
      121 agcctacccg ggttcagtac gggccgtacc ttatgaaccc ctatttggcc ttgctccggg
      181 tggagtttac cgtgccacgg actgttacca gccgcgcggt gcgctcttac cgcacccttt
      241 cacccttacc tgatcccgct tgcgcgggcc atcggcggtt tgctctctgt tgcactggtc
      301 gtgggtttcc cccccaggcg ttacctggca ccctgcccta tggagcccgg actttcctcc
      361 cctccgcccg tctcccccga agaggacgac gacgaagcgg cgactgtctg gtcagcttcg
      421 gcgcgcagta tagagggttt gcgcgccctt gtcaccccac gttgcgcatt ccaatcgcca
      481 gtgtcgcggc gatattacgt gaagcacggc agatattgtc ataagccccg cggaatgctt
      541 cgtccaacgt accaatgctg gtcagtacgc tgaagaccgc atcaatgcca tgctgatgta
      601 caacgccaac atcatcggtc aggctacccg caatgccaat caccggttta tggtacttct
      661 tcgccacgtt tgcgacacca atcggtacct tgccgtgaat actctggctg tcaatacgcc
      721 cttcaccggt gatcaccagc gtacaatcgt gaatatgttc ctccagattc agcgccgtag
      781 tgacgatttc aataccactt ttcagttccg caccaagaaa cgccatcagc gccgcgccca
      841 taccgccagc agctcctgcg ccggggacat ctttcacatc aacatgcagc gcttttttaa
      901 tgacatcggc atagtgagag aggttattgt ccagctcaac aatcatcgct tcactggctc
      961 ccttttgtgg gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat
     1021 cacaagcgac gcgaatgacg caatctttta agcgcggatc gaggccggaa atatcaatat
     1081 cattcagagt attaagacta ccgccgccaa aaccaatttc attgccgttg gcgtcgcata
     1141 atttcgcccc cagcgcctgt accatgcctg cgccgccatc atttgtagcg ctgccgccaa
     1201 tgccgataat aatgtttgtt gcgccgctct ccagcgcctg caggattaac tcgcctgtgc
     1261 cgcgtgaagt ggtcacgagt ggatcgcgtt tttccgcagg taccagctcc agcccactgg
     1321 ccgccgccat ttcaataaac gcggttttgc catcgccgga gatcccccaa ctggcattca
     1381 ctttctcgcc caacggccct gtaacccagg cgtgacgttc ggaaccctgg gtggctgcaa
     1441 tcatcgcttc caccgttcct tcgccaccgt cggcaaccgg aacagaaacg tactgtgcat
     1501 caggaaaaat ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg gcagataaac
     1561 tttctttata agagtctggg gcgattacga ttttcatacc tatgcctgtt accacatgac
     1621 gccggagggc gtttctctta ttcggcctgg attccaggcc cggattgcaa tacgccatcc
     1681 gggcacgacg tcattaacga gtaacttcga ctttcgccag tttttcgtag tagcacgcca
     1741 gggcgctatg atccgccgtt cctaaaccat ctgcacgcag tgcctgcatc atctccataa
     1801 ccgcagctgt gagcggcagt tgcgcgccga cgccgtgaga agtatccagc gcattcgcca
     1861 gatccttaat atgcagatca atgcggaagc ccggcttgaa gttgcggtcc atcaccatcg
     1921 gcgctttggc atccagcacg gtactgcccg ccagcccacc gcgaattgcc tgataaacca
     1981 ggtccgggtt aacgcccgct ttggttgcca gcgttaacgc ttctgacatc gcggcaatat
     2041 tcagcgccac aatgacctga tttgccagtt tggtgacgtt acctgcaccg atttccccgg
     2101 tatgcaccac ggaacccgcc atcgctttca tcaaatcata gtatttgtcg aaaatagcct
     2161 tgtcgccgcc caccatcact gacagcgtac cgtcgatggc tttcggttca ccgccgctca
     2221 ccggagcatc cagcatatca atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg
     2281 ccagcggtgc gatagaactc atatcgatca ataccgtacc tggcttcgcg ccttcaataa
     2341 tgccattctc acccagcgcc acctctttca catgagggga gtttggcagc atggttatga
     2401 tgacgtcgca ctgttcagcg atcgctttag ccgtagacgc tgtttctgca cctgcagcaa
     2461 tcacgtcagc aatagcttct gggttacggt cagcaaccac cagcgagtaa cctgctttca
     2521 gaaggttttt actcattggt ttacccataa tccccaggcc aataaaacca actttcatag
     2581 tcatatcaat catctctctt gttgcggtgg tggttatttt ttaaaggtat cagccagttt
     2641 ctgagtggca gagcggaaga cgccgagatc gctgccgaca gccacaaacg tcgcgcccca
     2701 ttccagataa cgacgcgcat cggcttcgac cggcgcgagg ataccgctgg gtttgccgtg
     2761 cgcgctggca cggttaaaaa tgtgctgaat tgctttttgt acatccgggt gtgatgcatt
     2821 gccgagatgg cctaatgccg cggccagatc gctggggccg acgaagatgc cgtctacgcc
     2881 ttcggtagcg gcaatggcat cgacgttatc tacgccctgc tgactttcta tctggaccag
     2941 aatagtgatg ttcttgttcg actaagcgaa ataatccgcc acggtgccaa acatattggc
     3001 gcggtgagaa acggagacgc cgcgaatgcc ttctggcgga taacgcgtcg atgccacggc
     3061 ctgctctgct tcctcttttg tttctacaaa aggaatcagg aagttataga aaccgatatc
     3121 cagaagacgc ttaataatta ccggctcgtt ggtcggcact cgcactactg gcgcgctggt
     3181 gctgcctttc agggccatta actgcggaat aaacgtggaa atatcgtttg gcgcatgttc
     3241 gccatccagc accagccagt caaacccagc caaaccaaga acttcagtgc taatcgggtt
     3301 agagagtgct gaccagcaac caatttgtac ttgtttcgca gccagtgcgg ctttgaattt
     3361 attcgggaaa acatcgttat tcatcgctta tacctttgct tatttctgca attccatacg
     3421 tttaatgtcg ccaactacga agaggtagca gaccatcgcc atcagcgctg aacatcccac
     3481 gaaaaccagt gctgcattga aggagtgcag ttcacttacc aggtagccaa tcaccagtgg
     3541 agtgacaatg gaggcaacat tgccaaagac gttaaagacg ccgccgcaga ggccaacaat
     3601 ctctttcggc gcggtgtcag aaatcaccgg ccagcccagc gcaccaaatc ctttgccaaa
     3661 gaaagccagc gccatcagca tgaccaccag cgtggtgttg ttggtgtagt tacataagat
     3721 gatggtggaa gccagcaaca ttcccagcac aatcggtagc ttacgtgcca gggtcaggga
     3781 caaaccgcgt ttgatcagat aatccgagaa gacacctccc agcacgccgc ccgcaaaacc
     3841 acacattgcc ggaatcgagg cgaccagacc cactttcaga atcgacatgc ctttttcctg
     3901 caccagataa atcgggaacc aggtgaggaa gaaccaggtg atagtgttga taaaatattg
     3961 tccgaaaaat acgcccaaca tcatgcggtt agagagcaat tgcttgatgt aatgcagttt
     4021 gggaccgctt gctgccgcgc tgcccggctt tttgtggtcc atatcgacca ccgcgccatt
     4081 ttcagagata aacttcagct cttccgcaga catacgtggg tgatctgtcg ggttatgaat
     4141 caacttgatc cacagcgccg tcagcacaaa accaatcacc cccataacgg taaagacgtg
     4201 ctcccagccc caggcgaaag tcagccagcc aagcagaggc gaaaagagcg ccagcgagaa
     4261 atattgcgcc gagttaaaga tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc
     4321 ggcaacaatt cgggcgttcg ccgggaatga tggcgcttcc gagaagccga gcataaagcg
     4381 cataaagaac atggagatcc cagcccaggc cagcgggaac atatcaacaa agccttgcag
     4441 gaaggtgaat agcgaccaga aaaagaggct gtaggtgtaa acttttttcg agccaaactt
     4501 atcaagcaac cagccgccgg ggatttgcat cagcaagtag gcccagccaa aagcggagaa
     4561 gatgtaaccc atcgaaaccg cacttaactg caactctttt gctacttcgg taccagcaat
     4621 agacagcgtt gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa tcagtaatat
     4681 taaatagcgg gtatgcacgc ctttcttttt ttcgtcaacg gtgtccagaa tcattttatt
     4741 tacctcgggt acttatgctg atttttatta ttatggggaa ggtgttattt atgagtttca
     4801 tttatgccgt aacgacaatg aactcgggaa ttagtataag cagcgcgaga ataataatca
     4861 ttgtgcaaat gctaatttaa ttaatactat ttaaatatta ttttgagcat atgcacataa
     4921 ggttgcgcgc taaagcacag atttgcgctt taccttgccg ggcggcactg caatccctga
     4981 aatgattgac attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat
     5041 gaagtcattt atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat
     5101 attgtacaat ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag
     5161 ttcacgacac agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc
     5221 gttttccgga tgggctggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc
     5281 tggacattcc ggctaatggt gaaattattc gttatggcga agtgattggt tacgccgtgc
     5341 gcgcaatccc acgcggaagc tggatcgacg agtcaatggt tgtactgccg gaagcgccgc
     5401 cattacacac gctgccactg gcaaccaaag tgccggaacc cttaccgcca ctggaaggat
     5461 acacctttga gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca
     5521 tcaccaccag cgtccactgt gtggcaggcg tggtggacta cgtagtaaaa atcattgaac
     5581 gcgatctgct accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg
     5641 gttgtggcgt ggcgattaac gcaccggcgg cagttgtgcc tatccgtacc attcacaata
     5701 tttcgctgaa ccctaacttt ggcggcgaag taatggtgat tggtctgggt tgtgaaaagt
     5761 tgcagcctga acgcctgctg actggaacgg atgatgtgca agctattcca gtagaaagcg
     5821 ccagcattgt cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata
     5881 ttttgcaggt cgccgaacgc catttgcaca aactgaatca acggcagcga gaaacctgtc
     5941 cggcttcaga actggttgtc ggcatgcagt gcggtggcag cgatgcgttt tctggcgtaa
     6001 cggcaaaccc ggcggttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga
     6061 tgttttcaga agtcacagaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga
     6121 acgaagaggt cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata
     6181 tgggaaaaac cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa
     6241 acgtggtaga gaaagcgctc ggctccattg ctaaatcggg taaaagtgca attgttgaag
     6301 tgctgtcgcc cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca
     6361 gcgattttgt ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga
     6421 ccggccgtgg tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcaacccgca
     6481 ccgagctggc gaaccgctgg tttgatttaa tggatattaa cgcgggcact atcgctaccg
     6541 gcgaagagag cattgaagag gtgggctgga agttgttcca ctttattctc gacgtcgcca
     6601 gcgggaagaa gaaaaccttc tcggatcaat ggagactgca taaccagctg gcggtgttta
     6661 acccagcacc ggtgacctga tttcttttgg aacgttcctc gcaaaacatg gccttagtgc
     6721 catgttttta ttgtttaaag tccccacgtc cattaataat gcatttgcat taccttagtt
     6781 caagcttata atttgagcag aaaacaggct gtaaaaggac agtgaatcat gcccgctaat
     6841 gctcgctctc acgctgtact gaccactgaa tcaaaggtca cgatacgcgg acaaacaact
     6901 atccccgcgc cagtgcgtga ggccttaaaa ctgaagccag gccaggacag cattcattac
     6961 gaaattctgc ctggtgggca agtatttatg tgccgactgg gagatgaaca ggaggatcat
     7021 actatgaatg catttttgcg ttttctggat gcagatatcc agaacaaccc gcaaaaaact
     7081 cgtccattca acattcaaca aggaaagaaa cttgtcgctg gcatggacgt caacattgat
     7141 gatgagattg gcgacgacga ataatggatt ttccacaaag ggttaatggt tgggcgctat
     7201 atgctcatcc ctgttttcag gaaacctacg acgctttagt tgccgaagtc gagacattaa
     7261 agggaaaaga tcctgaaaat tatcagagaa aagccgccac aaagttattg gcggtagtcc
     7321 ataaagtgat tgaggagcat atcacggtca atccatcatc accggcattc cgtcatggca
     7381 agtcgttagg ctctgggaaa aataaagact ggtcacgggt aaaatttggt gctggtcgtt
     7441 atcgtctctt ctttcgttat agtgaaaaag agaaagtcat cattctggga tggatgaacg
     7501 atgaaaacac tctgcgcacc tacggtaaaa aaacagatgc ctataccgta ttcagcaaaa
     7561 tgttaaaaag aggacatcct cctgccgact gggaaaccct cacccgagag acagaagaaa
     7621 cccattgatg gtgttcacaa tgcccgccga acgcattcgt gttgaccagc gggcaccctt
     7681 tctcactccc cgaccagaat cacttcaacc ccagcctttc gcagtccttc cagactatcc
     7741 gcaggaatgc cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt
     7801 aaactggaac gattgaattt actggaatcg gtgaccacga tgatccgttc cgcaacttcg
     7861 cacatccgac ggtttaaacg ggcttcatct tcattatgtg tgctgacgcc gcgctccaga
     7921 tcgatcgcat ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgctca
     7981 gcctgatcgc cgtaaaaaga ttgcgactga cggcgcaaat gcccgccggt catcagcagc
     8041 tcaacgcctt ccgcttccag caacgcatta gccacgttca taccgttggt catcgcaatt
     8101 acgtcagtgt gcttgcgcat cagacgagca atctcaaaag tggtggtccc ggaatcgagg
     8161 atcacccgat gaccaggctg aatcaactca acggcagctt tcgcaacgct gcgtttcatc
     8221 gcggtgttca gtgcgctttt atcttccact gatggctcga ctgacggcgt cgtgctatcg
     8281 cagatcaacg cgccaccata ggcacgcaca gcgatcccct gcttttccag aaacgccaga
     8341 tcgttgcgga tcgtcacagt agatacgcca tacaatgccg acagatcgtt aacctgcaca
     8401 ctcccttgct gtcgcagacg ctgaatgatc tgttctcgtc gctcgctggt gcctgtcact
     8461 cgcttctcac ctgaagcgtc ggtattactc atagtaagtc ctttcgtaaa actttcgttt
     8521 catttcgttt tgcctattaa cgcctttcta ttaagcaaat gcaagcccac cttgcccatt
     8581 gacgcaagct actctcgttt cagtgacttt cattatgttt cttttgtgaa tcagatcaga
     8641 aaaccattat ctttcgtttt atttttatat caccatgacg cagtatcaac tgaaacaaaa
     8701 cgaaagatta atatcgcagt aatctgaact ggagaggaaa gtgaaacatc tgacagaaat
     8761 ggtgagacag cacaaagcgg gcaaaacaaa tgcaatttat gccgtttgtt ccgcacatcc
     8821 gctggtgctg gaagctgcaa tccgctacgc cagtgcaaac caaacgccgt tactgattga
     8881 agcaacctcc aatcaggtag accagttcgg cggttatacc ggaatgacgc ccgccgattt
     8941 tcgcggcttt gtttgtcagc tcgccgactc gttgaatttc ccgcaggatg cgttgattct
     9001 gggtggtgac catctggggc caaaccgctg gcaaaacctg ccagccgctc aggcaatggc
     9061 caatgccgat gatttgatta aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga
     9121 ttgcagcatg tcctgtcagg acgatccaat tcccttaact gatgacatcg tggctgaacg
     9181 cgccgcccgt ctggcgaaag tggcggaaga aacctgtctt gaacactttg gcgaagccga
     9241 tctggagtat gtcattggta ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag
     9301 cgagctggcg gtcaccacgc cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc
     9361 ctttgaaaag caaggtttga atgccatctg gccacgcatc attgccctgg tggttcaacc
     9421 cggcgtcgaa ttcgatcaca ccaacgttat tgattatcag cccgccaaag cgagcgcctt
     9481 aagccagatg gtcgaaaact acgaaacgct gattttcgaa gcgcactcta ccgattatca
     9541 aacgccgcaa tcgctgcgcc agctggtgat tgaccacttt gccattctga aagttggccc
     9601 agcgctgacc ttcgccctgc gtgaagctct gttctctctg gcggcgattg aagaagaact
     9661 ggtgccagcg aaagcctgtt ctggtctgcg tcaggtgctg gaagacgtga tgctcgaccg
     9721 cccggaatac tggcaaagcc actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg
     9781 ttatagctac tcggatcgcg tgcgctatta ctggccggac agccagattg atgacgcttt
     9841 cgctcatctg gtacgtaatc tggcggattc accaattccg ctgccgctga tcagccagta
     9901 tctgccgctg cagtacgtga aagttcgctc cggcgagctg cagccaacgc cacgggaact
     9961 cattatcaac catattcagg acatcctggc gcagtaccac acagcctgtg aaggccaata
    10021 agcaaaacaa agaggaacac gctatgccaa atattgtttt aagccggatt gatgaacgct
    10081 tgattcacgg tcaggtcggc gttcaatggg tcggatttgc gggggcaaat ctggtgctgg
    10141 tagccaacga tgaggttgcc gaagatccgg tacaacaaaa cctgatggaa atggtactgg
    10201 cagaagggat cgccgtacgt ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg
    10261 ccgccgatcg acagaaaatc ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg
    10321 tgaaaggtgg cgttccggtg aatcgcatta acgttggcaa tatgcactac gccaatggca
    10381 aacaacaaat cgccaaaacg gtttctgtgg atgcgggcga tatcgcagca tttaacgacc
    10441 tgaaagccgc tggggtggaa tgcttcgttc agggcgtccc gacagagcct gctgtggacc
    10501 tctttaaatt actttgaggg attcatcatg gaaatcagcc tgttgcaggc atttgcgttg
    10561 ggcattatcg cctttatcgc tggcctggat atgtttaacg gcctaacaca tatgcaccgc
    10621 ccggtggtcc tcggcccgtt ggtcgggctg gtacttggcg atctgcatac cggaatttta
    10681 accggcggta cgctggaact ggtgtggatg gggctggccc cgctggcggg cgcacagccg
    10741 cctaacgtga ttatcggtac tatcgtcggc acggcgtttg ccattactac tggcgtgaaa
    10801 cccgatgtcg cagtcggtgt cgccgtacct ttcgctgtcg cagtacagat ggggattacc
    10861 ttcctgttct cggtgatgtc cggcgtgatg tctcgctgcg accggatggc ggagaacgcc
    10921 gatactcgcg gcattgaacg cgtgaactat ctggcactgc tggcactcgg cactttctat
    10981 tttctctgcg ctttcctgcc tatctacttc ggcgcggaac atgcaaaaac catcattgat
    11041 gtcctgccgc aacgattaat cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc
    11101 ggttttgccg tgctgctgaa aatcatgatg aaaaacgtct acatccccta cttcatcctg
    11161 ggctttgttg ccgcagcctg gctcaagtta ccggtgctgg ctattgctgc cgctgcgctg
    11221 gcgatggcgc tgatcgacct gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag
    11281 aaagaggaat ttgaagatgg catctaatca aaccaccctg ccgaacgtca gtgaaaacga
    11341 agaaacattg ctgactggcg tcaatgaaaa cgtgtatgaa gatcagaaca ttggcgcgga
    11401 gctgacgaaa aaagatatca accgcgtcgc ctggcgttcc atgctgttac aagcgtcttt
    11461 taactacgaa cgtatgcagg cttctggctg gttgtacggt ctgctgcctg ctctgaaaaa
    11521 gatccacact aataaacgcg acctggcgcg cgccatgaag gggcatatgg gtttcttcaa
    11581 tacccatccg tttctggtga catttgttat cggcattatc cttgcgatgg agcgttctaa
    11641 gcaggacgtt aacagtattc agagcaccaa aattgccgtc ggtgcgccgc tcggcgggat
    11701 tggcgatgca atgttctggc taacgctact gccgatttgt ggcgggattg gagccagtct
    11761 ggcattgcaa ggctccattc ttggcgcagt cgtctttatt gtgctgttca acgtggtaca
    11821 cctggggctg cgttttggtc tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc
    11881 actgattaaa gcgaatacca aaaaagtcgg ccatgctgcg tctatcgttg ggatgacggt
    11941 aatcggtgcg ctggtggcaa cctatgttcg tttaagcacc acgctggaaa tcacggcggg
    12001 cgacgcagtg gttaagttac aggctgatgt tatcgacaaa ctgatgccgg ccttcttacc
    12061 gttggtctac accctgacca tgttttggtt ggtacgccgc ggctggagtc cgctgcgtct
    12121 gattgcagtt accgtggttc tcggcatcgt cggtaaattc tgccatttcc tttaaataca
    12181 atgaggtatt agatgttaag tattattttg acagggcatg gcgggtttgc cagcggcatg
    12241 gaaaaagcaa tgaagcaaat tctcggtgag caatcgcagt ttatcgccat cgattttccg
    12301 gaaacctcaa gcaccgcgct gcttacatcg cagcttgaag aggccatcgc ccaactggat
    12361 tgtgaagacg gcattgtttt tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct
    12421 tcaacgctgg cgatgcaaaa accgggctgt gaagtgatca ccggcaccaa tttgcaactg
    12481 ctactggaga tggtgctgga gcgcgaaggg ttaagcggtg aagaattccg tgtacaggca
    12541 ctggagtgtg gacatcgtgg gctgaccagt ctggtggacg agttaggccg ctgccatgaa
    12601 gaatgtccgg tcgaggaagg gatatgacac acgttctgcg cgccagaagg ctgctgactg
    12661 aagagggatg gctcgatgac catcagttgc gtattgctga cggtgtcatc gcagcaatcg
    12721 aaccgattcc agtgggcgtg actgaacgcg atgcggaact gctctgcccc gcttacatcg
    12781 acacccatgt acacggtggt gcgggcgttg atgtaatgga tgacgcgccg gatgtactcg
    12841 acaagctggc aatgcacaag gcacgcgaag gtgtcggcag ttggttgccg actaccgtaa
    12901 ccgcgccgct taacaccatt catgcggcgc tgaaacgtat tgctcaacgt tgccaacgcg
    12961 gcggacctgg tgcgcaagtg ctggggagtt atctcgaagg accgtacttc acgccgcaga
    13021 ataaaggcgc gcatccgccg gagttgtttc gcgagcttga aattgccgag ctggatcaat
    13081 tgattgccgt ttctcagcac accttacgcg tggtagcgct ggcaccggaa aaagaggggg
    13141 cattgcaggc catccgccat cttaaacagc aaaatgtacg agtgatgctg gggcatagcg
    13201 cggcgacctg gcaacaaact cgcgccgcgt ttgatgctgg tgccgacggc ctggtgcatt
    13261 gctataacgg gatgacaggt ttacatcacc gcgaaccggg aatggttggc gcgggattaa
    13321 cggacaagcg cgcctggctg gaactgatag ccgatggtca tcatgtgcat ccggcggcga
    13381 tgtcgctgtg ttgttgctgt gcaaaagaga gaatcgtgct gatcaccgac gcgatgcagg
    13441 cagccgggat gccggatggt cgctatacgt tatgtggcga agaagtgcag atgcacggtg
    13501 gcgttgtccg taccgcgtct ggtgggctgg cgggcagtac gctatctgtc gatgcagcag
    13561 tgcgcaacat ggtcgagtta acgggcgtaa cgcctgcgga agccatccat atggcgtcgc
    13621 tgcatccggc gcgaatgctg ggtgttgatg gtgttctggg atcgcttaaa ccgggcaaac
    13681 gcgccagcgt cgttgcgctg gatagcgggc tgcatgtgca acaaatctgg attcagggtc
    13741 aattagcttc gttttgatgg tttgctccat tattgggcct tcacttcccc cgtaaggcct
    13801 ttctttttct ttcgttttga tctgtgcagc ggtgtcggat gcgacgctaa cgc
//
LOCUS       DACAPB010000126        13841 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.126, whole genome shotgun sequence.
ACCESSION   DACAPB010000126 DACAPB010000000
VERSION     DACAPB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.126"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(91..618)
                     /gene="mobB"
                     /locus_tag="IT904_003234"
     CDS             complement(91..618)
                     /gene="mobB"
                     /locus_tag="IT904_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAP3192975.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDRAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(600..1184)
                     /gene="mobA"
                     /locus_tag="IT904_003235"
     CDS             complement(600..1184)
                     /gene="mobA"
                     /locus_tag="IT904_003235"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAP3192976.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1254..1523
                     /locus_tag="IT904_003236"
     CDS             1254..1523
                     /locus_tag="IT904_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAP3192977.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1600..2586
                     /gene="srkA"
                     /locus_tag="IT904_003237"
     CDS             1600..2586
                     /gene="srkA"
                     /locus_tag="IT904_003237"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAP3192978.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2603..3229
                     /gene="dsbA"
                     /locus_tag="IT904_003238"
     CDS             2603..3229
                     /gene="dsbA"
                     /locus_tag="IT904_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAP3192979.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            3384..4814
                     /locus_tag="IT904_003239"
     CDS             3384..4814
                     /locus_tag="IT904_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAP3192980.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVGKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(4855..5787)
                     /locus_tag="IT904_003240"
     CDS             complement(4855..5787)
                     /locus_tag="IT904_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP3192981.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(5907..6149)
                     /locus_tag="IT904_003241"
     CDS             complement(5907..6149)
                     /locus_tag="IT904_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3192982.1"
                     /translation="MSVPVYQIMGKGCHRQDKNMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKAIPNN"
     gene            6151..8937
                     /gene="polA"
                     /locus_tag="IT904_003242"
     CDS             6151..8937
                     /gene="polA"
                     /locus_tag="IT904_003242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP3192983.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTRRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(9319..9951)
                     /locus_tag="IT904_003243"
     CDS             complement(9319..9951)
                     /locus_tag="IT904_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP3192984.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            10533..11039
                     /gene="yihI"
                     /locus_tag="IT904_003244"
     CDS             10533..11039
                     /gene="yihI"
                     /locus_tag="IT904_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAP3192985.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            11228..12601
                     /gene="hemN"
                     /locus_tag="IT904_003245"
     CDS             11228..12601
                     /gene="hemN"
                     /locus_tag="IT904_003245"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP3192986.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(12791..12901)
                     /gene="yshB"
                     /locus_tag="IT904_003246"
     CDS             complement(12791..12901)
                     /gene="yshB"
                     /locus_tag="IT904_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAP3192987.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(13013..>13841)
                     /gene="glnG"
                     /locus_tag="IT904_003247"
     CDS             complement(13013..>13841)
                     /gene="glnG"
                     /locus_tag="IT904_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAP3192988.1"
                     /translation="LNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFL
                     DEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFRED
                     LFHRLNVIRVHLPPLRERREDIPRLARHFLQVAAHELGVEAKLLHPETEAALTRLAWP
                     GNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADR
                     ALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME"
BASE COUNT         3740 a         3307 c         3428 g         3366 t
ORIGIN      
        1 cgaaaggcca tcctgacgga tggccttttt gcgttggtgc aaaaaaatgc cggatgcgac
       61 gctggcgcgt cttatccagc ctgctcttgc ttatccgtcc tgcttttgca tccactccac
      121 cacaaaatca gccagcccct caacatcatt aatatccagt aatgcgacat caagattaag
      181 cggcacatca ctggctacag caataacatg tctgtctatc actaattctt ccggtcgatg
      241 tccggctcta tcgcgaaaca gcacaatctt tgcgatctct tcatgcttaa acccttcgac
      301 cagaatcaaa tccagctttg aggtatccat tcgacttgcg agaaaatgta gatccagctc
      361 ttcttcgtct ggtgtttccg tcatcaaggc ccatcgttgc tggctggcaa cgatggtttg
      421 tgccgcgcca gccttgcgca gctcatagct atctttgcct ggcttatcaa catccatatc
      481 atggtgcgta tgcttaatca gccctggacg gatccctctg gcgcataatg ccgggatcag
      541 ttgtttcaaa agcgtagttt ttccggtacc actccacgcg gcaaaggcga gtaacggtat
      601 catcgttttt cctgccatcg ggcaagctcc tctggcgtat tcacgttaac aaatgcatct
      661 ttatgatcgc tgaaatcaac cgcatgaccg ccagccagac gcataaatgc cattacccgg
      721 cgttctcctg cttgcagata ttctagtaat aaaggctcaa tagcgcggtt taccagagca
      781 atagtcgggt gatcgcgttc accgtcatgg acccacacga caggcgcatc tttgcgctga
      841 tgattaagcc gggcggctaa atcatgggga atgtaaggcg tatcgcacgg acaaaacaaa
      901 aaccactcac cagcttcctg ctgcattact gaaagcatcc ctgccagagg gcctgggtaa
      961 tccgccagtg aatcttcaat cactttcaga ccgcctgcct gatagatttc ctgatgacga
     1021 ttagcattaa ccacgacgtg agagagctgc gtcataagcg cgtcagcgac atgttgccat
     1081 aatggtttgc cgtttaattc aagcaatcct ttatctacgc cgcccattcg tctggcttta
     1141 ccgcctgcca gcacaacgcc tgttatcgtc gtcatcagat tcaccgatat cgcctctttt
     1201 attgtgggat tgaccctgct aacgtgtctg tctcaagagt aaggagcatt actatgaaat
     1261 gtaaacgtct gaatgaagtt attgaactcc tccagccagc ctggcaaaaa gagccagacc
     1321 ttaacctgtt gcaatttttg cagaaactgg cgaaagagtc aggttttgac ggcgaactgg
     1381 cggatttgac ggatgacatt ctgatctatc acctgaaaat gcgtgattcc gcgaaagatg
     1441 cggtgatccc gggtttgcag aaagattatg aagaagactt caaaacggcg ctgttacgcg
     1501 ctcgcggcgt aattaaagag taaaagcttg taagcggcgc caccaaaatc atcgtgaaat
     1561 gatatccttc gtcattcgta atgttttcca gatgatggga tgaataacag cgcttttact
     1621 ttccagacac tacacccgga taccatcatg gacgctctgt ttgagcatgg gatccgggtg
     1681 gattccggtc ttaccccgct taacagctat gaaaaccgtg tctatcaatt tcaggacgaa
     1741 gatcgtcgac gttttgtcgt caaattttat cgccctgaac gttggacagc cgatcaaatc
     1801 ctcgaagaac atcaatttgc gttgcagctg gtaaatgatg aagttccggt cgcagcacct
     1861 gtggccttta acggtcagac tttattgaat catcagggat tttatttcgc tgtttttcca
     1921 agcgtcggtg gtcgccagtt cgaagctgat aatatcgatc agatggaagc ggttgggcgt
     1981 tatttagggc gtatgcacca gacggggcgc aaacagcttt ttatccatcg cccgaccatc
     2041 ggtttgaacg aatatctcat tgagccacgc aagctgtttg aggacgctac actgatacct
     2101 tccgggttga aagcggcatt cctgaaagcg acagatgagc tgattgccgc cgttacagca
     2161 cactggcggg aagatttcac cgttctgcgg ctacatggag actgccacgc cgggaatatt
     2221 ctctggcgcg atggtccaat gtttgttgat ctggatgatg cacgtaatgg tccagccgtt
     2281 caggatttgt ggatgttgct caatggcgat aaagccgagc agcggatgca actggaaact
     2341 attattgaag cttatgaaga atttagcgag ttcgacaccg ctgaaatcgg actgattgaa
     2401 cctttacgcg ccatgcgttt ggtttattat cttgcctggc taatgcggcg ttgggctgat
     2461 cccgcgttcc cgaaaaattt cccgtggtta accggggaag attactggct gcgacagacg
     2521 gcgactttta tagaacaggc aaaagttcta caagaacccc ctttgcaatt aacacctatg
     2581 tattaatcgg agagagtaga tcatgaaaaa gatttggctg gcgctggctg gtttagtttt
     2641 agcgtttagc gcatcggcgg cgcagtatga agatggtaaa cagtacacta ccctggaaaa
     2701 accggtagct ggcgcgccgc aagtgctgga gtttttctct ttcttctgcc cgcactgcta
     2761 tcagtttgaa gaagttctgc atatttctga taatgtgaag aaaaaactgc cggaaggcgt
     2821 gaagatgact aaataccacg tcaacttcat gggtggtgac ctgggcaaag atctgactca
     2881 ggcatgggct gtggcgatgg cgctgggcgt ggaagacaaa gtgactgttc cgctgtttga
     2941 aggcgtacag aaaacccaga ccattcgttc tgcttctgat atccgcgatg tatttatcaa
     3001 cgcaggtatt aaaggtgaag agtacgacgc ggcgtggaac agcttcgtgg tgaaatctct
     3061 ggtcgctcag caggaaaaag ctgcagctga cgtgcaattg cgtggcgttc cggcgatgtt
     3121 tgttaacggt aaatatcagc tgaatccgca gggtatggat accagcaata tggatgtttt
     3181 tgttcagcag tatgctgata cagtgaaata tctgtccgag aaaaaataat tcaatgtaaa
     3241 ttacataaag cccgtgaata ttcacgggct ttttttatta tttaataaat ataaatacat
     3301 tctgataatg catcctgccg ctggacatta tttcagcata agtggatatt caaagttttg
     3361 ctgttttatc agggaatatt tatatgatac gtaagtcagc tacaggtgtt attgttgcgt
     3421 tagccgtaat ctggggtggt ggcacatggt acacaggtac gcaaattcag cctggtgtcg
     3481 gaaaatttat taaagatttt aacgatgcta aaaagaaagg tgaacatgcc tacgatatga
     3541 cgttaagtta tcaaaatttt gacaaaggct tttttaattc ccgttttcaa atgcaaatga
     3601 cattcgataa cggtgcaccc gatctcaata tcaagccagg ccagaaagtt gtatttgatg
     3661 tggatgttga gcacggtccg ttgcccatca caatgttaat gcatggtaat gtcatcccag
     3721 cactggcagc ggcaaaagtg aacttagtga ataatgaact gacacaaccg ctatttatcg
     3781 ccgcgaaaaa taaatcgccc gtggaagcga cattgcgatt cgcgtttggt ggctcattct
     3841 ctacgacatt agatgttgcc cctgcagagt atggaaagtt ttcttttggt gagggccagt
     3901 ttacttttaa tggtgatggt agttcattgt ctaacctgga tattgaaggc aaagtcgaag
     3961 atattgttct gcaattatca ccaatgaaca aagtaacggc aaaaagtttt accattgatt
     4021 ctctggcgcg attagaagaa aagaaatttc cggttggtga aagcgagtcg aaatttaatc
     4081 agattaacat tatcaatcac ggggaagacg ttgcccaaat cgatgctttc gttgcaaaaa
     4141 ccatgctgga tcgcgttaaa gacaaagatt atatcaatgt caatctgacc tacgaacttg
     4201 ataagttaac aaaagggaat cagcaactcg gtagtggtga gtggtcattg attgctgaat
     4261 ctattgatcc ctcagcggtg cgccaattta tcatccagta taacattgca atgcagaagc
     4321 agcttgctgc acaccctgag ttagcaaacg atgaagttgc tctgcaagaa gtgaatgctg
     4381 cattgttcaa agagtattta ccgttattac aaaaaagtga gccgaccatt aaacaaccgg
     4441 taaaatggaa gaacgcactc ggcgaactaa atgccaatct ggatatcagt attgccgacc
     4501 cagccaaatc ttcatcatcc acaaacaaag atattaaatc gctcaatttt gatgtgaagt
     4561 taccgcttaa tgtcgccaca gaaaccgcaa aacagcttaa tttatctgaa ggaatggatg
     4621 cggaaaaagc gcaaaagcgg gctgataaac aaatcagcgg gatgatgacc ttaggtcaga
     4681 tgtttcagtt aatcacgatt gacaacaata ccgcctcgct gcaactgcgt tatacaccgg
     4741 gtaaagttgt ttttaacgga caggagatga gcgaagaaga atttatgtct cgtgccggac
     4801 gttttgttca ttaaaatact cactcgcctc tgctcattca gaggcgagca aaatttactt
     4861 gtcctgccgc tgtgatgaca tcaacgacgt cagaagtcgg tctttttcct gccacagtga
     4921 gttcagccat tgctgaaaat ggcgcttaaa gcttttgtca ttgatgtagt cgccatgtaa
     4981 ctcatcggca ataggctgta aatccacatg tacgacaatc cgcgtcagtt taccgcttaa
     5041 catatcgaag aacggctgac ggttattgtc cggataacac agcgtaacat taagcagttt
     5101 atcgaattgt ttacccagta catttagcgc catcgcaatg cctgccgcct ttggtggcaa
     5161 caagttttga aaagtggagt gggtttgctg atgtttttct tgcgtgaaac gggagccttc
     5221 aacgaaattg acaatggtgg tgggatgcag gcgaaacttt tcacaagaac gccgagtggt
     5281 ttcaacatct ttaccacgtc gctccggatg acgtaataaa taagcgcggg aataacgctt
     5341 cataaacggc atatccagcg cccagcacgc caggccaaga aatggcaccc aggccagctg
     5401 ttgcttgagg aaatatttat tcatcggaat gtgcttacga aacagcacgc acagtacgac
     5461 aatatctgcc caactacggt ggttacaaat aagcagatac cagttcttct tacttaaccc
     5521 ttccagcccg tgaacttccc attgcaggtg tgggttcaga tgcagtaata ccgccagacc
     5581 ttcacaccag caatacatca taaaatcaca aaaacgcgaa acctttcgcc aaatgactgg
     5641 cacaggtagc aatagtttta caatcccggc aatgatgatc gggacggagc aaaaaatagt
     5701 gaccaaaatg gtcaatacga tactcagcaa aagggttatc gcagcgagta ttctcgtcat
     5761 aatgaattta ttcaaaaggt tagccataga cagtgcgcta aagaagcgca aggcgcagat
     5821 tttagcagaa aacgacccaa ataacggatg atccttaagg agaaaaataa ttcatatcta
     5881 tccacattag aaaaaatccc attatctcaa ttattaggga tggctttatt tttaactgca
     5941 tgaaaaacaa agacaaacat catgctgtaa aaagcatgat aataaattaa aagcgatgta
     6001 aataatttat gcacaaagtt atccacatga cgatttgcga gcgatccaga agatctacaa
     6061 aagattttca cgaaaagcgg tgaaaaactc atgtttttat cctgtctgtg gcatccttta
     6121 cccataatct gataaacagg cacggacatt atggttcaga tcccccaaaa tccacttatc
     6181 cttgtagatg gttcatctta tctttatcgc gcatatcacg cgtttccccc gctgactaac
     6241 agcgcaggcg agccgaccgg tgcgatgtat ggtgtcctca acatgctgcg cagtctgatc
     6301 atgcaatata aaccgacgca tgcagcggtg gtctttgacg ccaagggaaa aacctttcgt
     6361 gatgaactgt ttgaacatta caaatcacat cgcccgccaa tgccggacga tctgcgtgca
     6421 caaatcgaac ccttgcacgc gatggttaaa gcgatgggac tgccgctgct ggcggtttct
     6481 ggcgtagaag cggacgacgt tatcggtact ctggcgcgcg aagccgaaaa agccgggcgt
     6541 ccggtgctga tcagcactgg cgataaagat atggcgcagc tggtgacgcc aaatattacg
     6601 cttatcaata ccatgacgaa taccatcctc ggaccggaag aggtggtgaa taagtacggc
     6661 gtgccgccag aactgatcat cgatttcctg gcgctgatgg gtgactcctc tgataacatt
     6721 cctggcgtac cgggcgtcgg tgaaaaaacc gcgcaggcat tgctgcaagg tcttggcgga
     6781 ctggatacgc tgtatgccga gccagaaaaa attgctgggt tgagcttccg tggcgcgaaa
     6841 acaatggcag cgaagctcga gcaaaacaaa gaagttgctt atctctcata ccagctggcg
     6901 acgattaaaa ccgacgttga actggagctg acctgtgaac aactggaagt gcagcaaccg
     6961 gcagcggaag agttgttggg gctgttcaaa aagtatgagt tcaaacgctg gactgctgat
     7021 gtcgaagcgg gcaaatggtt acaggccaaa ggggcaaaac cagccgcgaa gccacaggaa
     7081 accagtgttg cagacgaagc accagaagtg acggcaacgg tgatttctta tgacaactac
     7141 gtcaccatcc ttgatgaaga aacactgaaa gcgtggattg cgaagctgga aaaagcgccg
     7201 gtatttgcat ttgataccga aaccgacagc cttgataaca tctctgctaa cctggtcggg
     7261 ctttcttttg ctatcgagcc aggcgtagcg gcatatattc cggttgctca tgattatctt
     7321 gatgcgcccg atcaaatctc tcgcgagcgt gcactcgagt tgctaaaacc gctgctggaa
     7381 gatgaaaagg cgctgaaggt cgggcaaaac ctgaaatacg atcgcggtat tctggcgaac
     7441 tacggcattg aactgcgtgg gattgcgttt gataccatgc tggagtccta cattctcaat
     7501 agcgttgccg ggcgtcacga tatggacagc ctcgcggaac gttggttgaa gcacaaaacc
     7561 atcacttttg aagagattgc tggtaaaggc aaaaatcaac tgacctttaa ccagattgcc
     7621 ctcgaagaag ccggacgtta cgccgccgaa gatgcagatg tcaccttgca gttgcatctg
     7681 aaaatgtggc cggatctgca aaaacacaaa gggccgttga acgtcttcga gaatatcgaa
     7741 atgccgctgg tgccggtgct ttcacgcatt gaacgtaacg gtgtgaagat cgatccgaaa
     7801 gtgctgcaca atcattctga agagctcacc cttcgtctgg ctgagctgga aaagaaagcg
     7861 catgaaattg caggtgagga atttaacctt tcttccacca agcagttaca aaccattctc
     7921 tttgaaaaac agggcattaa accgctgaag aaaacgccgg gtggcgcgcc gtcaacgtcg
     7981 gaagaggtac tggaagaact ggcgctggac tatccgttgc caaaagtgat tctggagtat
     8041 cgtggtctgg cgaagctgaa atcgacctac accgacaagc tgccgctgat gatcaacccg
     8101 aaaaccaggc gtgtgcatac ctcttatcac caggcagtaa ctgcaacggg acgtttatcg
     8161 tcaaccgatc ctaacctgca aaacattccg gtgcgtaacg aagaaggtcg tcgtatccgc
     8221 caggcgttta ttgcgccaga ggattatgtg attgtctcag cggactactc gcagattgaa
     8281 ctgcgcatta tggcgcatct ttcgcgtgac aaaggcttgc tgaccgcatt cgcggaagga
     8341 aaagatatcc accgggcaac ggcggcagaa gtgtttggtt tgccactgga aaccgtcacc
     8401 agcgagcaac gccgtagcgc gaaagcgatc aactttggtc tgatttatgg catgagtgct
     8461 ttcggtctgg cgcggcaatt gaacattcca cgtaaagaag cgcagaagta catggacctt
     8521 tacttcgaac gctaccctgg cgtgctggag tatatggaac gcacccgtgc tcaggcgaaa
     8581 gagcagggct acgttgaaac gctggacgga cgccgtctgt atctgccgga tatcaaatcc
     8641 agcaatggtg ctcgtcgtgc agcggctgaa cgtgcagcca ttaacgcgcc aatgcaggga
     8701 accgccgccg acattatcaa acgggcgatg attgccgttg atgcgtggtt acaggctgag
     8761 caaccgcgtg tacgtatgat catgcaggta cacgatgaac tggtatttga agttcataaa
     8821 gatgatgttg atgccgtcgc gaagcagatt catcaactga tggaaaactg tacccgtctg
     8881 gatgtgccgt tgctggtgga agtggggagt ggcgaaaact gggatcaggc gcactaagat
     8941 tcgcctgaac atgccctttt ttcgtaagta agcaacataa gctgtcacgt tttgtgatgg
     9001 ctattagaaa ttcctatgca acaactgaaa aaaaattaca aaaagtgctt tctgaactga
     9061 acaaaaaaga gtaaagttag tcgcgtaggg tacagaggta agatgttcta tctttcagac
     9121 cttttacttc acgtaatcgg atttggctga atattttagc cgccccagtc agtaatgact
     9181 ggggcgtttt ttattgggcg aaagaaaaga tccgtaatgc ctgatgcgct atgtttatca
     9241 ggccaacggt agaattgtaa tctattgaat ttacgggccg gatacgccac atccggcaca
     9301 agcattaagg caagaaaatt attcgccgtc ctgcgtttct tctacaggct gcatctcgct
     9361 aaaccaggta tccagtttct gccgcagctt gtccacgcct tgtttcttca acgaagaaaa
     9421 cgtttcaacc tgcacatcac cgttaaacgc cagtacagct tcacgcacca tattcaattg
     9481 cgctttacgt gcgccgcttg ccagtttgtc cgctttggtc agcagcacca gaacggcgat
     9541 attgctgtct accgcccact caatcatctg ctgatccaaa tctttcagcg gatggcgaat
     9601 atccattagc accaccagac cttgcaggct ctgacgtttt tcgaggtatt cgccgagcgc
     9661 acgctgccat ttgcgcttca tctcttccgg gacttccgca taaccgtacc caggcaagtc
     9721 aaccagacgc ttgccgtcag ccacttcaaa caggttgata agctgggtgc gccctggggt
     9781 ttttgaggta cgagccaggc ttttctggtt agtcagcgtg ttcagcgcgc tggatttacc
     9841 tgcgttggaa cggcctgcaa aagccacttc aattccggta tcggaaggta ggtggcgaat
     9901 atcaggcgca ctcatcacaa aatgcgtctg ttgataattc aaattagtca aagcggtcgt
     9961 ctccgtcagt caaagctttg tggcgattat acctgaacca taataaaagg cggatttttc
    10021 ggcgtgagcg ttgtaagtaa aagccatacg ctttgtgaga cattgccgat agtctttatg
    10081 cgaaatagca gagaaaattc tgcgatgcat gtcaaataag ctatataaat cagtgaattg
    10141 cttttatata aaactctgaa aatgtgcaaa tactgatggc ggttgattgt ttgtttaaag
    10201 caaaggcgta aagtagcacc catagagcga ggacgctaac aggaacaatg actcaggatg
    10261 agggtcagga gcgccaggag gcgaagacag aggattgtca ggaagacaaa cgtccggaga
    10321 cgtaattaaa cggaaatgga atcaacacgg attgttctgg ctaatggaaa aacagggtgt
    10381 gttggcggcc tgcaaggatt gtaagacccg ttaagggtta tgagtcagga aaaaaggcga
    10441 cagagtaatc tgtcgccttt tttctttgct tgctttctgt tagattccgc cgcaaatcta
    10501 tactgaataa aacggctaaa agacgaacca ttatgaaacc atcttcacca tcacgcagca
    10561 aaggtcatgc aaaagcgcgt cgcaaaacgc gcgaagagct gaatcaggaa gctcgtgacc
    10621 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggtaaca
    10681 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
    10741 ctattccatt gggcgtgact gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
    10801 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
    10861 tgctggaacg tctggaagca ggcgaaaccc tgagtgccga agagcaatcc tgggtggatg
    10921 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
    10981 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggcaactaac
    11041 ggattgccgc catgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
    11101 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
    11161 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
    11221 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
    11281 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
    11341 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
    11401 tatcccgttc tgccataaac tttgttactt ctgcggttgc aataagattg ttactcgcca
    11461 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
    11521 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
    11581 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
    11641 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
    11701 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
    11761 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
    11821 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
    11881 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
    11941 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
    12001 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
    12061 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
    12121 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
    12181 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
    12241 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgcgctgtg
    12301 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
    12361 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
    12421 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
    12481 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
    12541 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
    12601 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
    12661 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
    12721 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
    12781 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
    12841 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
    12901 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
    12961 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
    13021 ttcccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
    13081 cttcctgttt atgcccctgc gtatgtcgca acgcggtcgt cagtaacgtc cgctccagct
    13141 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
    13201 gcgcaagaag cgtcgcccaa ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
    13261 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
    13321 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
    13381 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg tgcgcggcaa
    13441 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
    13501 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
    13561 ctcgctgttc gagattctgg tgagtggcag cgataatccg cacatccact ttcaccggcg
    13621 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
    13681 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
    13741 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
    13801 ctgattcgat caaatctttt gggatagctg ccatattcag c
//
LOCUS       DACAPB010000127        13723 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.127, whole genome shotgun sequence.
ACCESSION   DACAPB010000127 DACAPB010000000
VERSION     DACAPB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.127"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..101
                     /locus_tag="IT904_003248"
     CDS             <1..101
                     /locus_tag="IT904_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP3192989.1"
                     /translation="LARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(287..1549)
                     /locus_tag="IT904_003249"
     CDS             complement(287..1549)
                     /locus_tag="IT904_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAP3192990.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(1747..1822)
                     /locus_tag="IT904_003250"
     tRNA            complement(1747..1822)
                     /locus_tag="IT904_003250"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1787..1789),aa:Phe,seq:gaa)
     gene            complement(1928..2635)
                     /locus_tag="IT904_003251"
     CDS             complement(1928..2635)
                     /locus_tag="IT904_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP3192991.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3033..5168
                     /locus_tag="IT904_003252"
     CDS             3033..5168
                     /locus_tag="IT904_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAP3192992.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLCQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5218..6474)
                     /locus_tag="IT904_003253"
     CDS             complement(5218..6474)
                     /locus_tag="IT904_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAP3192993.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6676..7755)
                     /gene="mltC"
                     /locus_tag="IT904_003254"
     CDS             complement(6676..7755)
                     /gene="mltC"
                     /locus_tag="IT904_003254"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAP3192994.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7820..8095)
                     /locus_tag="IT904_003255"
     CDS             complement(7820..8095)
                     /locus_tag="IT904_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAP3192995.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8123..9175)
                     /gene="mutY"
                     /locus_tag="IT904_003256"
     CDS             complement(8123..9175)
                     /gene="mutY"
                     /locus_tag="IT904_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP3192996.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPA"
     gene            9336..10055
                     /gene="trmB"
                     /locus_tag="IT904_003257"
     CDS             9336..10055
                     /gene="trmB"
                     /locus_tag="IT904_003257"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP3192997.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10055..10381
                     /locus_tag="IT904_003258"
     CDS             10055..10381
                     /locus_tag="IT904_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAP3192998.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10565..11284
                     /locus_tag="IT904_003259"
     CDS             10565..11284
                     /locus_tag="IT904_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAP3192999.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11460..12506
                     /gene="ansB"
                     /locus_tag="IT904_003260"
     CDS             11460..12506
                     /gene="ansB"
                     /locus_tag="IT904_003260"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAP3193000.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12623..13630
                     /locus_tag="IT904_003261"
                     /pseudo
     CDS             12623..13630
                     /locus_tag="IT904_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
BASE COUNT         3503 a         3298 c         3576 g         3346 t
ORIGIN      
        1 atctggcaag gctgggacgg aagtcactgt cattctcaaa atcggtggag ctgcatgaca
       61 aggtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacctggt
      121 ttttgatgcc agccataacg tgtttgacta caaagtatta cgaaagaact ttaatgagcc
      181 cctggaataa ttcattaaag taatttactt cgctatcctt tttccagtta ctcacaaaga
      241 aaaatattca gaaaataatt cccgggctgg ctgcccggga tgattactat tttaatggat
      301 tgttaatctt tgcatattca aacgggctga tacattgttc tctgttcgca tccagaaaat
      361 ctgcccacca ctgtagcata agtcgacgtt cttccagatg ttcggcttta tggatatacg
      421 cagcacgtac accattacgc tcctgatggc tcatctggcg ttccacagca tcgcgggacc
      481 acaaacctga ttctattaat gcactacatg ccatcgcccg gaatccatga ccacagacat
      541 cctgggttgt gtcatacccc ataaccctca gggctttatt tacggtgttt tcactgattg
      601 gtttacgtgg gtcatgtgct cccgtaaaga tcagaccatt ttcacctgcc catgtctgaa
      661 gttctgccag tatggctaca gcctgttggc tgagtggcac aagatgtggt gttcgcatct
      721 tggagccccg gtatgaaaat tttacgcctg gcagaggttc gcgttcggct ggaatagtcc
      781 acatggcatt gtcaatatca atttcagacc agcgggcata tctgagttca ctggaacgaa
      841 taaaaaccag taaattcagc ataactgcca gtcgggtgac aggctggcct ttataaccgt
      901 caatacgcga cagaagttca ggcaggcgtg aaagatccag agcagggcga tgctggcgtt
      961 ttaccgtggt caatgcacca gccatatcca gagccgggtt atatctgatg atgccagact
     1021 gtacggcata acgcattaca gcagatattc tttgctgcag acgactggcg atttcataaa
     1081 tttgtttagc ttctgcagca cgaacaggga taagcaggtc cggggtactg agtgttgtga
     1141 tatcccggtt gccgataaag gggaaaacgt gtgtctcgag gcttttgagt actttatcgc
     1201 tgtgtgattg tgcccattgt ttattactgg catgccagcg tcgtgcaata tgttcaaaag
     1261 agagttgtaa atcagggact acttttttag ctcttttctg ttcgcaaggg tcaaccccct
     1321 ccgaaatcag ttttcgggcc tcatcccgtt ttgtacgagc ttcggagaga gaaacttccg
     1381 gatacacacc aagtgccagg gttttctcct tacccagaat acgataacgg agacgccagt
     1441 aacgggaacc attaggatga accagcagga acatgccggc cccgtcagtg agtttataag
     1501 ccttgtcagt gggctttgca gcccggattt ttgcgtcagt cagtgccata gttcagcctt
     1561 ctgaatgcgt taaagagggt acaaattctg tcgaactggg gtatacccgc aattataccc
     1621 acacaatggg gttgatgtgg ggggaatctg gttgagttcg gtagaattga tttggaggca
     1681 gaacgcttaa attgtggcgt tctgaaacga aaaacggacc tccgtggagg tccgttgata
     1741 tgaatttggt gcccggactc ggaatcgaac caaggacacg gggattttca atcccctgct
     1801 ctaccgactg agctatccgg gcaacggggc gcattaaacc tgattcgcct cgtctcgtca
     1861 atcaaatttc ttcaatttac tgcagactgc acaatctatc atcactttgc cgatattgca
     1921 cgcattgtca ggcaaaccag gcggtccagg cggcggaaag cggcattgcc agaaggagtg
     1981 ctggaagcat gttgaccacc gggaacattt taatgccaca gatgcgtaat ccggttgcca
     2041 gcagcaataa accgcctacg gcgctgaagt ctgccatcat cgacggtgtg gtcagcggta
     2101 aaatcagcgc ggcagcccac gccagcgtta actggatgat cagtaatggg atactaatca
     2161 ccgataccgc aatacccagc gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg
     2221 acttggcgat taaaatactc ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc
     2281 cggtgccgct ggcgcaaaac aggacaataa tcgcgacata attctgaata aaagattcat
     2341 gcgctggctt cttacgtgag tgacgaaaca gattttgtgc tttggcgact gctgtattga
     2401 cacctttctc cagcaaacaa atttcgccaa ttagagcgcc aagtagcgta gctaaaacca
     2461 tcgctggaag gttggcacat ttcaccacca gtaaaatacc aatccccagc gatgccagac
     2521 caaaaataga tgtcatggag acgcggatac gttccggtaa gcgttggctg agcagtgcgc
     2581 cgagaacgcc acccagtaag actgcactag cgttgataaa agggccgatg accacaagag
     2641 ttcctgttaa ctgtattgtt tgattttctt attatgaact ttttgccttg ttgatggctt
     2701 gtctggtcgc aaagtccagg cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt
     2761 ttctcctctc tgtacggagt ttgcccgatg catgccatct ccttacattc tctcgcttat
     2821 cgccgtttcg cgcgaaactc ttctcttttt atgctactgc tcatgcggtg aagttaacgc
     2881 acgttcactg caggacaaca gtaaaatcag agcgtttctg cttttactga tgtctggcgg
     2941 tcggagctgg tgaccagttt gacccatatc tcatggggca gggttttaca ccttgtccag
     3001 tattcttact tccccgaaac gggtttgcgc ttatgaaatc aatgaatatt gccgccagta
     3061 gtgaactggt atcccgactt tctactcatc gccgcgtggt ggcgttggga gatactgatt
     3121 ttacggacgt cgcggcagtc gtcattaccg ctgcggatag tcgcagtggc attcttgcgt
     3181 tgcttaagcg caccggtttt catctaccgg tgtttttgta ttccgaacat gctgttgaat
     3241 tacctgcggg cgttacggcg gtaatcaacg gcaacgagca gcagtggctg gagctggaat
     3301 ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt
     3361 acgttgagat gggcaacagc acctttgctt gccctgggca tcaacatggt gcgttcttta
     3421 aaaagcatcc tgccggacgc catttttacg atttctttgg tgaaaacgtc tttcgcgccg
     3481 atatgtgtaa tgctgacgta aaattgggcg atctgcttat tcatgaagga tcggcgaaag
     3541 atgcgcagaa attcgcagcc aaagtctttc atgccgataa aacctatttt gtgctgaacg
     3601 gcacatcggc agcgaataaa gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc
     3661 tcttcgaccg taacaaccat aagtcgaatc atcacggcgc gctgattcag gcgggggcga
     3721 cgccggtcta tctggaagct tcacgcaacc cgtttggttt cattggcggt attgatgcgc
     3781 actgttttaa tgaagagtat ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg
     3841 acctgccgcg cccgtttcgc ctggcgatta ttcagctggg aacctatgac ggcactgtct
     3901 ataacgcccg tcaggtgatc gataccgttg ggcatctgtg tgattacatt ctgtttgatt
     3961 ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc ggatagctcg ccgctgctat
     4021 tagaacttaa cgaaaacgat ccggggatct ttgtgactca gtcggtgcac aaacagcagg
     4081 cgggattctc acagacgtcg cagatccata aaaaagataa ccatatccgc ggacaggcgc
     4141 gtttttgccc gcataagcgg ttgaataacg cctttatgct ccatgcttct accagccctt
     4201 tctatccgct gtttgctgca ctggacgtta acgccaaaat tcatgaaggg gagagtgggc
     4261 gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc gcgcaaggct attcttgcgc
     4321 gctgtaagct gttccgcccg tttatcccgc ccgttgttga tggcaaattg tggcaggatt
     4381 atccgacatc tgtgttagcc agcgaccgcc gttttttcag ttttgagccg ggggcgaagt
     4441 ggcacggctt tgaaggatat gccgcggatc agtattttgt tgatccgtgc aagctgttac
     4501 tcactacacc aggtatcaat gccgaaaccg gcgaatatag cgactttggc gttccggcga
     4561 cgattctggc gcactatctg cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact
     4621 ccattctgtt cttattaact ccggcggaaa gccacgagaa gctggcacaa ctggtggcga
     4681 tgctggcgca gtttgaacag catattgagg atgactcgcc gctggctgag gtgttgccga
     4741 gcgtttataa caaatatccg gtgcgctatc gcgactacac tttgtgccag ttgtgtcagg
     4801 agatgcacga tctgtatgtc agtttcgacg tcaaagacct acaaaaagcg atgttccgcc
     4861 agcagagttt cccgtcagtg gtgatgaacc cccaggatgc gcatagcgct tatattcgcg
     4921 gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg aattgcggca gaaggggcgt
     4981 tgccttatcc acctggcgtg ctttgcgtgg tacccgggga agtctggggt ggggcggttc
     5041 aacgttattt ccttgcactg gaagaagggg tgaatttgtt gccgggattt tcgccggagc
     5101 tgcaaggtgt ttatagcgaa accgatgcgg atggcatgaa acggttgtac ggttatgtgt
     5161 tgaagtaaga ataaaaaaaa cgggtcacct tctggcgacc cgtttttctt tgcgtaatta
     5221 gtggctaacc gtctgtgtgc ctgtcgggac acgaacgtgt ttatatttga acatcgccat
     5281 gaacgcgaag gccagaacca cggagtaacc agcgaaaatc aaccatacgg tttgccagtc
     5341 ggtaatgccg ttttgggtgt acatctcaac aactttaccg ctcacgatgc cgccgaggat
     5401 acagccgaag ccgttagtca tcatcaggaa catcccttgt gcactggcgc gaattgccgg
     5461 gctaacttct ttttcgacaa acaccgaacc agagatgttg aagaagtcga atgcgcaacc
     5521 gtaaacgatc atcgacagta ccagcagtac agtaccgaac ggagtcgggt cgccgtaagc
     5581 aaacagcgca aaacgcagga tccacgccac aatactgatc atcattacgt tcttaatacc
     5641 gtagcggctt aagaagaacg ggatggtcag aatgaacagg gtttcagaga tctgcgaaat
     5701 cgacatgatg attgacgcat gctgcacaat aaagctgctg gcaaacatcg gatctttgtc
     5761 gaagctgtgc aggaaggtat taccgaacat gttggtaatc tgcagttccg cgcccagcag
     5821 cattgagaag ataaagaaga ttgccatacg cttgttttta aacagcgcga atgcatcgag
     5881 gcccagcagg gttgtccagc tctgattcgc ttgctgttta gcaaccggaa tatgcggcag
     5941 agtcagggta aacagaacca gaatggcgga aagtgctgcg ccaatataca gctgcatgtg
     6001 gcttaattcg aagccagaca ggctcaccac ccacattgcc atgataaagc cgatggtgcc
     6061 ccagatacgg attggcggga agtcagtaac gatatccatc ccggcatttt gcaggcgata
     6121 gtaagagatg gtgttgatta acccaagcgt tggcatataa gcaaacgagt taatcaatat
     6181 cacaaggaac atcgcttccg gcgtcgtgac ctgtgccgcc atgaacagcg tgatagcgcc
     6241 aatggtgtgg caaatggcat atacccattt cgcacttaac catttgtcgg ccacaatccc
     6301 cagcagcgca ggcataaaga ccgctgcgat acccagtgag ctataaactg cgccaataga
     6361 agcaccgtca aacttcaggg taacaaacat ataggagccg agggtcgtca gccaacttcc
     6421 ccacagacag aactgcagaa aagagaggat tttcagctgc agcttaagat tcatgttaat
     6481 ttcctcacat cgtgatgcgg atgagtgttt gaaaaagcac atttgaccgt gcgagatgat
     6541 gggattctat caacttcgcg gactggtttt ttgttacctg tggcaaataa ttgcaaacat
     6601 tcccaatttg caaaacgaaa ttgtgatgtg gataacattt ttgcccctga gcatcgtcag
     6661 gggcggttaa tggaattatc ggcggcggta ggatttttgc gcggtattca ctttataaag
     6721 ataacggcga gattccgcag agggatggcg ggtcgtcagc gtctgataaa catcgcccgg
     6781 cgtcatggtg ttaataatat tggcagcctg aatcttatca ttcgaaaaga ctcgcagcac
     6841 gctgcctgcg ccgccgttat aggcggtgat gacggcataa cgccgcgatg ttgggttatc
     6901 aattccgccg agataaacat tgttcagcat cgccagatac gcggtgccgg tatcaatatt
     6961 gctggcagga tcaaacaaga aactgcggct cggcgtgccg gatttcccct gcgagcggaa
     7021 cacatctttc ccggcagtat gttgtaccac ctgcattaat cccagcgcat cggaacggct
     7081 gaccgcatac gggttaaagg aagattcggt ctgcataatt gccagaatca gcgactcatc
     7141 aacgccatat ttccgtgacg cctggcggac catgccgaga tatttgtgcg cacgtttatc
     7201 aaggtggttc ggcaccatgt taatggtgac gctgtagatg atacgcagcc cgttgctgcg
     7261 gctcttcaga cggtttttca gcagataatc cgcgaagttg cttgcgcgac cttcccagcg
     7321 aatcggctgc ccggtgttgt ccaccacctg accgtaaagg aaaggttctt tcgaaatcgt
     7381 aatatcatca acgtcggaat agagatcgac cgaactcgga tcgtcaccca tcaataacgt
     7441 tttgataatt gcccggcgca aatgcgcggc aggttctgtc ccggcgatgg tttcgatagt
     7501 aatcgtaccg tcatcgaagt tgatgtggct gcgggtctga tattgatcgg tgtatttcac
     7561 gtagtcctta ggaccagcga tcaccacctc tttgaagccc cagatgttct caatattgtg
     7621 ggcaaattgc cccatcagaa tatcaaaacc gttggtatct ttgacccagg cttcgttata
     7681 ggtatcgcct tttttggtcg tcgaacagga gatgagcaac ggcgcaatca aagccagcgc
     7741 gagatatttt ttcatcattc cgggtgcgtg ttgtgtttgt ttttatgcaa gttgccggag
     7801 gcgtgctccg gcactgtttt tattttttat cttccggcgt atagccctcg atatgcacct
     7861 ctttaccctc gaacaggaag ttgaccatct cctgctcaag cagcttgcgg tgctcggcat
     7921 tcatcatgtt gagtttcttt tcattaatca gcatggtttg cttgtgctgc cactgcgccc
     7981 aggcttcttt ggagatctcg ttatagatgc gttttcccag ctcgccgggg tacagctgaa
     8041 aatcctgacc ttctgcttca cgttgcagga aagtacaaaa aatcgttctg ctcataaatc
     8101 atcctcttta tcgactcacg cgctaagccg gcgcgccagt gcgtaactgc tgtaacaaac
     8161 gctccacggg agccgccagg ccaactgacg gcggttgcgc taagttatac cagagcgcat
     8221 tgccttcatc catgcagccg gtgaatgacg acacgggaag ccacataggc acaatatcta
     8281 agtggaaatg gctgaaggta tgccgaaacg cggtcagttg cgtcaggtta tcggcagcaa
     8341 tctgccgttg cgccagccac tgccgcaaac tttcttcgtc ggcaaactgc gggaaacagt
     8401 ataaaccgcc ccacaatccg ctcggcggac gctgcgccag caatacttca tcttcgtgct
     8461 gtaatagcaa aaagtagccg gtgcgctccg gcagcgtctg tttcggtttt ttgcccggat
     8521 aaagcgccca gctattgttg gcggcggcaa tacatccgtt ttgtagcgga cagagcgaac
     8581 atttcggttt cgagcgcgta caaatcatcg cacccaaatc catcatcgcc tgattaaacc
     8641 gttccacgcc aaccgcgggc gtcacctgct cgctcaaact ccataattta ttctcgacct
     8701 cttttttccc aggccagccg cttacagcat agcagcgcgc cagcacgcgt ttgacgttac
     8761 cgtcgagaat cggaaagtgc ttacccagag aaagcgagag aatcgcgcct gcggtggaac
     8821 gcccgacgcc cggtaacgcc gcgacttctt caaaggtttc cgggaattta ccgccgtgta
     8881 aggtcgccac ttgttgtgcc gctttatgca gattgcgcgc gcgggcgtaa tagccaagcc
     8941 cggtccacaa gtggagaact tcgtcgagcg gcgcattggc gagatcggtc accgtcggga
     9001 agcgcgccat aaagcgttca aaatagggga taacggtcgc aacctgagtt tgttgcaaca
     9061 tcacttctga gagccatact ttgtagggcg tcttgtcaat ttgccagggc agcgtttttc
     9121 gcccgtattt atcgtaccag tccagaacct gggctgaaaa ttgcgacgct tgcatggtca
     9181 ccgaattcac tgttgttggg ggcaagattg cagcacagcg acggcagggt gtaaaccgga
     9241 actttccgca gccacggcct tatcatgctt gcatccggca actaactttg gataatgccc
     9301 gttttcagaa cactttcaca agcgactaaa tctttatgaa aaacgacgtc atttcaccgg
     9361 aatttgatga aaacggccgc ccactgcgcc gtatccgtag ttttgtgcgc cgccaggggc
     9421 gactgaccaa aggccaggaa catgcgctgg aaaactactg gccggtgatg ggcgttgagt
     9481 tcagcgaaga tatgctggat ttccccgcgc tttttggccg tgaagcgccg gtgacgcttg
     9541 agattggttt tggcatgggg gcgtcgctgg tggcaatggc taaagatcgc cctgagcagg
     9601 acttcctcgg cattgaagtg cattcaccgg gcgttggtgc gtgcctggct tctgcgcatg
     9661 aagaaggttt aagcaacctg cgagtgatgt gtcacgatgc ggttgaagtg ctgcataaaa
     9721 tgattcctga caattcattg cgcatggtgc agctcttttt ccctgacccg tggcacaaag
     9781 cgcgccataa taaacgccgt atcgtacagg tgccgtttgc cgaactggta aaaagcaaac
     9841 tgcagctggg gggcgtattc catatggcga ccgactggga accttatgcg gaacatatgc
     9901 ttgaagtgat gtcttctatt gacggttata aaaacctgtc agagagcaat gattacgtac
     9961 cgcgtccggc atcacgtccg gtgacgaaat ttgaacaacg tggtcatcgt cttggtcacg
    10021 gagtatggga cttaatgttc gagagggtga aataatggca aagaaccgta gccgtcgtct
    10081 gcgtaaaaaa atgcacatcg acgaattcca ggaattagga ttttcggtgg catggcgatt
    10141 cccggaaggt acatcggaag aacagattga taaaaccgtt gatgatttta ttaacgaggt
    10201 tatcgaaccg aacaaactgg cctttgacgg cagcggttat ctggcttggg aaggtctgat
    10261 ctgcatgcag gaaatcggca aatgcaccga agaacatcag gcgattgtgc gtaagtggct
    10321 ggaagagcgc aaactggatg aggtacgcac cagcgaactt ttcgacgttt ggtgggacta
    10381 agaaagcata cgggcgatga caaatgcaaa actgcctgat gcgctacgct tatcatgcct
    10441 ggaaagatgc acgatcgagt aggcgggata aggcgtttac gccgcatccg gcatggaaaa
    10501 cgcgtacttt gttatcaatc tggggccagc aaatgctggc ctgatttgtt cttgagggaa
    10561 gactatgatg cgcaaaatgc tgctggcggc agcactttca gtgacggcaa tgaccgctca
    10621 cgctgactac cagtgcagcg tcacgccgcg tgacgatgtg attgtcagcc cgcaaaccgt
    10681 gcaggtgaag ggcgaaaacg gcaatctggt gatcacgcca gacggcaacg tgatgtataa
    10741 cggtaagcaa tattccctga atgccgccca gcgcgagcag gcgaaggatt atcaggctga
    10801 actacgcagc acgctgccgt ggattgatga aggcgcgaaa agccgcgtcg aaaaagcccg
    10861 tattgcgctg gataaaatta tcgttcagga gatgggcgaa agcagcaaaa tgcgcagccg
    10921 tctgaccaaa cttgatgcgc agctgaaaga gcagatgaac cgcattatcg aaacgcgcag
    10981 cgatggcctg acgtttcact ataaagccat tgatcaggtt cgcgccgaag gccagcaatt
    11041 agtgaatcag gcaatgggtg gaattttaca ggacagcatt aatgaaatgg gcgcgaaagc
    11101 ggtgctgaaa agcggcggta acccattaca gaatgtgctg ggaagcctgg gggggctgca
    11161 atcctcaatc caaaccgagt ggaaaaagca ggaaaaagat ttccagcagt ttggcaaaga
    11221 tgtttgtagc cgcgttgtga ctctggaaga tagccgcaaa gccctggtcg ggaatttaaa
    11281 ataatcctct attttaagac ggcataatac ttttttatgc cgtttaattc ttcgttttgt
    11341 tacctgcctc taactttgta gatctccaaa atatattcac gttgtaaatt gtttaacgtc
    11401 aaatttccca tacagagcta agggataatg cgtagcgttc acgtaactgg aggaatgaaa
    11461 tggagttttt caaaaagacg gcacttgccg cactggttat gggttttagt ggtgcagcat
    11521 tggcattacc caatatcacc attttagcaa ccggcgggac cattgccggt ggtggtgact
    11581 ccgcaaccaa atctaactac acagcgggta aagttggcgt agaaaatctg gttaatgcgg
    11641 tgccgcaact aaaagacatt gcgaacgtta aaggcgagca ggtagtgaat atcggctccc
    11701 aggacatgaa cgataatgtc tggctgacac tggcgaaaaa aattaacacc gactgcgata
    11761 aaaccgacgg cttcgtcatt acccacggta ccgacacgat ggaagaaacc gcttacttcc
    11821 tcgacctgac ggtgaaatgc gacaaaccgg tggtgatggt cggcgcaatg cgcccgtcca
    11881 cgtccatgag cgcagacggt ccattcaacc tgtataacgc ggtagtgacc gcagctgata
    11941 aagcctccgc taatcgtggc gtgctggtgg tgatgaacga caccgtactg gacggtcgcg
    12001 atgtcaccaa aaccaacacc accgacgtag cgaccttcaa gtctgttaac tacggtcctc
    12061 tgggatacat tcacaacggt aagattgact accaacgtac cccggcacgt aagcacacca
    12121 gcgatacgcc attcgatgtc tctaagctga atgagctgcc gaaagtcggc atcgtttata
    12181 actacgctaa cgcatccgat cttccggcta aagcactggt agatgcgggc tatgatggca
    12241 tcgttagcgc tggtgtgggt aatggtaacc tgtataaatc cgtgttcgac accctggcaa
    12301 ccgccgcgaa aaacggcact gcagtagtgc gttcttcccg cgtaccgacg ggtgctacca
    12361 ctcaggatgc tgaagtggat gatgcgaaat acggcttcgt cgcctctggc acgctgaacc
    12421 cgcaaaaagc gcgcgtcctg ctgcagctgg ctctgacgca aaccaaagat ccgcagcaga
    12481 tccagcagat cttcaatcag tactaatcac ctcgccccgg tatcgtgccg gggctttttc
    12541 acttcagact tacgtccatt gccaattttc attaccctaa tgataatcac cggaataaat
    12601 tattccgcgt gaggtttttc gggtgaaaaa gcaatggatt gttggcacgg cactgcttat
    12661 gttgatgact ggtaatgcct gggcagatgg cgaaccgcca actgaaaata tcttaaaaga
    12721 tcaattcaaa aagcagtatc acggcattct caagcttgat gctatcacct taaaaaatct
    12781 tgatgctaag ggtaatcagg ccacctagtc agcggaaggc gatgtctctt ccagtgacga
    12841 tctctatacc tgggtcggtc agttggcaga ttacgaactg ctcgaacaga cctggacgaa
    12901 agataaaccg gtaaaattct cggcgatgtt aaccagtaaa ggaacgcccg cgtctggctg
    12961 gtcggtgaac ttttactctt ttcaggcggc agccagcgat cgtgggcggg tggtcgacga
    13021 tatcaaaacg aataataaat atctgatcgt gaatagcgaa gatttcaatt atcgctttag
    13081 tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg aaaaggaagt
    13141 gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat attggggaaa
    13201 agatgcaaac ggtaagcaaa tgacccgcga agacgccttt aaaaaaattc accaacagcg
    13261 tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg acaaagaggt
    13321 ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg aagtgccgat
    13381 tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc tgcaatcaca
    13441 aaaacttagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc aatatccgtt
    13501 aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga aaattgacga
    13561 tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc gcaatggggt
    13621 gattaagtaa gtttgatata gtgcacgatt tgtagcagga tgcggcgttg accccgcatc
    13681 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gac
//
LOCUS       DACAPB010000128        13553 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.128, whole genome shotgun sequence.
ACCESSION   DACAPB010000128 DACAPB010000000
VERSION     DACAPB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.128"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(103..1248)
                     /gene="glxK"
                     /locus_tag="IT904_003262"
     CDS             complement(103..1248)
                     /gene="glxK"
                     /locus_tag="IT904_003262"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAP3193001.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGEINLFNSARNIACAIKIGQGIKN"
     gene            complement(1270..2571)
                     /locus_tag="IT904_003263"
     CDS             complement(1270..2571)
                     /locus_tag="IT904_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAP3193002.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(2628..3989)
                     /gene="allB"
                     /locus_tag="IT904_003264"
     CDS             complement(2628..3989)
                     /gene="allB"
                     /locus_tag="IT904_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAP3193003.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(4049..5503)
                     /locus_tag="IT904_003265"
     CDS             complement(4049..5503)
                     /locus_tag="IT904_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HAP3193004.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(5672..6550)
                     /gene="glxR"
                     /locus_tag="IT904_003266"
     CDS             complement(5672..6550)
                     /gene="glxR"
                     /locus_tag="IT904_003266"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP3193005.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(6650..7426)
                     /gene="hyi"
                     /locus_tag="IT904_003267"
     CDS             complement(6650..7426)
                     /gene="hyi"
                     /locus_tag="IT904_003267"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAP3193006.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKHVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(7439..9220)
                     /gene="gcl"
                     /locus_tag="IT904_003268"
     CDS             complement(7439..9220)
                     /gene="gcl"
                     /locus_tag="IT904_003268"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAP3193007.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(9310..10125)
                     /gene="allR"
                     /locus_tag="IT904_003269"
     CDS             complement(9310..10125)
                     /gene="allR"
                     /locus_tag="IT904_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAP3193008.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(10203..10685)
                     /gene="allA"
                     /locus_tag="IT904_003270"
     CDS             complement(10203..10685)
                     /gene="allA"
                     /locus_tag="IT904_003270"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAP3193009.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            10915..11841
                     /gene="allS"
                     /locus_tag="IT904_003271"
     CDS             10915..11841
                     /gene="allS"
                     /locus_tag="IT904_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAP3193010.1"
                     /translation="MFDPETLRTFIAVAETGGFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            11910..13004
                     /gene="mnmH"
                     /locus_tag="IT904_003272"
     CDS             11910..13004
                     /gene="mnmH"
                     /locus_tag="IT904_003272"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAP3193011.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
BASE COUNT         3601 a         3615 c         3188 g         3149 t
ORIGIN      
        1 gaatttgcgc gattttcagg aaggatatag tactcatgcc gcatccggca tgagtactgc
       61 gctcttatac gaaataaata gcatccgctt taaaggcaag agtcagtttt taattccctg
      121 acctatttta atggcgcagg caatatttcg cgcgctgttg aagagattga tttcaccgct
      181 ggcgagtact tcggctaaag gtgccagacg tggcaaaatg ctgaataccg catcaatgcc
      241 atactggtgc accacttcca cgccatcacc caatacgcca gcaatcccaa tcaccggtac
      301 attaaactgc ttcgccaccg acgccacacc cagcggcgct ttaccgcctg ccgtttgcga
      361 gtcgatgcgc ccttccccgg taatcaccag tgctgcgccc tgcactgcct gcgcaagatt
      421 gaccgcattc aacacaatct caatacctgg tttaatatcc gcattgagaa ataccgccgc
      481 cgcaataccc ataccgcccg cagcgccgcc gcccgccatc tggcagacgt tgatttcagt
      541 ttgctgttgt aaaacacggg cgtaattttg cagcccctgt tccagctctt cgaccatttc
      601 cggcgttgcc cctttttgcg ggccaaacac cgccgccgcg ccgcgtgccc ctaccagcgg
      661 gttatcaacg tcacatgcta cttcaatatg gcaattcgcc agacgcggat cgcattcatc
      721 catctcaatg cttgccacgc gcgctaaatt accgccgtta gccaccagca cctgaccgtc
      781 ggcatcaagg aaacgcacac cgagcgcctg cgccatgccc ataccgccgt cgaccgtcgc
      841 actgccgcca atgccgagaa taatatggcg aatgccgtta tccagcgcat gacgaattaa
      901 ctcccccgta ccaaaactgg aggccagcaa cggattacgc ttttcaggcg cgaccagcat
      961 caggccactt gctgccgcca tctcaatcac cgccgttttc ccgtcgccgg taaggccata
     1021 aaaagcattc actgtttcgc ccatcggccc gcagacttca agcgtcacga tgttgccgcc
     1081 cgtcgcggcg accatcgcat ccaccgttcc ttcgccgcca tccgctatcg gcaaacagat
     1141 atagtgcgca tcgggaaaga gggtcgaaaa cccggcttta attgcctggc aacatttttc
     1201 tgcacttaag ctctctttaa aagagtctgg cgcaatgaca atcttcatta tcaatccttt
     1261 ttcaacaaat tattcgatgc gttcccacgg aataagatta tccataagaa cagccagtaa
     1321 aatcccgacc aataagccgt tactgagcag aggacgcagc gttaatggaa ggtcttgcag
     1381 atacacaggc ggtaatgcca ttaaaaatat gccgacaaat aacggcaatg cgagtcgata
     1441 aatattgcga gcggtaaagg ttatttgctg gctaaacacc agcgcggaaa agagtaaagg
     1501 cagataagaa accagcatga ccgcgctact cacgggtaaa gggatactgc aaaacagtcg
     1561 tgtgagtgcc ggaaccagcg ccaccagcag gcaaataacg ctgccataaa taaacgaacg
     1621 ccgcgtgtaa tcgccagtct gggttaataa accaatggat gaaacaaacg gtgaaaatgg
     1681 aattaccgcc agcggtacgg ttatcagcgt cataaatccg gtcgccacaa agctacgacg
     1741 ataacgcgta ttccccgcgc cctgctgcgg ataaaaaaca tccgtgcccc gaatcgcacc
     1801 gtaggtattg ctgatattca ccagacctgt aatcaccgcc gtcagaataa ttcccggcga
     1861 caaagcaccg ccactgccga gcgggaacca ctgccagtgc aactcaccgg agagcgaatg
     1921 cgaagaagga aagcaaaagt accacaacaa ccagccggtt atcgtaccga ccagcaggcc
     1981 ataacgggca aaacgttgcg gcagaaagat aatcatcgcc agtaccaggc acatcaccgc
     2041 caccgagagc gcgaacggtg gtaactgaat tttaaaattt gggtcggcta tgccaaacgg
     2101 cagcccgagc atacctttga aaaagatagt ggtcagctgc gcgcccagca tcaacataaa
     2161 caagaccatc accgacggcg taaataaccg tgccaggcga tggcctaatc cgctaaaacc
     2221 aatcaacatc gtcagcacgc cggagagtgc aatccccacc gccagactgg tagcgatatc
     2281 gttgatcggt gtcccgcgcg atgcttcgcc aagggtgata gtaaggatgg ttccccacca
     2341 caggccaccc ggcccttcca taatagcgcg acgatgaccg cagaacgcct gagcgaagca
     2401 ggccagtgcg gtagcaagaa aagcatattg cgtgagcgta agcaggctac tttgcggcaa
     2461 ctgaaaagca gaaagtagcg taggaggaac cacaaccgtg ttgcaaaaaa taaagaaaaa
     2521 ccactgaaat cctgataaca ggctttcgcg gctgactgca aaattgaaca taaacacacc
     2581 ttaaccggag aatgcccgcc gcaaggacgg gcattgcagg ggccagatta ctgctgatgt
     2641 ttaaggataa attgaccttt cggcgcaaca gggaagccct gttcaatgtc gtaaatcaca
     2701 tcaccacgta agatggtttt cgtgatacgc gcgccaatgg tacggccaac atacgggctg
     2761 actttgtggc gatattccag atcgtcattg gtaagaacat agctgctatt cggctgaatg
     2821 aagacgaagt cggcatcttt tcctggggcg atacggcctt tttgctgcag accgaaaata
     2881 tctgctgcgt tagtcgccat taatttgccg aacattggca gagacattcc gcgtttctgt
     2941 accgcttcat cgaacatcac gtccatacag ttttgcagac cggcaatacc gccccatgct
     3001 tccatgatgt tgccggcttt catttccgga gggcatggtg agtggtcgga aaccaggcag
     3061 tcgatttcac cgttaaacag tttttcccac atgcctttct ggttttccag atcgcggatc
     3121 ggcggtgaac acttcgccag agtaccgatt tcttcgaact gatcggtatc cagtacaaag
     3181 taatgcgggc aggattcaca agtaacatcc tgaccttcct gacgtgcacg agtcacttct
     3241 tcaacacctt ctgggctgct gatatggcaa atgtgcagac ggcaaccggc aactttcgct
     3301 aggtacagta cgcggcgaat cgcttccact tcggtaaata ccggacgcga agccacatag
     3361 tcatgggcag ttacgcgacc ttcacttttc gcttcttcgc ccagtgcatc acagatcagc
     3421 gcgttttcgc agtgcaccag caccggctgc cccagttcgc ccagcttctg cgcacctttg
     3481 aagaactgcc agtcgtttac atcacggaag tcgttgtcga taccgcgatc gccacaggtc
     3541 gcaacgaagc atttgaagcc gacaacgccc acttcatcca actcatgcag acgatcaatg
     3601 ttgtaagaca ccaggccacc gagttgtgcc gcatcgatag tcagcttgcc tttagcggca
     3661 tcgaacttca gttcaattga agcacggtca accgttgcag gcagctggtt gagcggcatt
     3721 tcgatcatgg tggtgatacc accttttgcc gctgcgcgag taccggtttc ataaccttcc
     3781 cagtggctac gacccggttc agaaatatgg gtgtgcgcat caaccatgcc cggcgaaacc
     3841 accagaccag acgcatccat aacttctttt gcatcgccca gatcctgacc gatagcagca
     3901 atttttccgc ctttaacggc gatatctaca acgcgagctt cgttttctaa aataacggtg
     3961 ccgtttttaa tgattaaatc aaaagacata acaaactcct tgtaaaatac agattggttt
     4021 ttaactcaat gttcaatatc gggattaatt aacctatggt tttttgctct cctgtttttt
     4081 ctgctgttgt acgtttcttt aataaggcgt aggccgcaaa ggcgacgatg acgccgacaa
     4141 accatgaaac acgcgataac ggttccataa agtgaataaa cttaccgcca agagataaaa
     4201 taacggccac cagagttact gaaaacgcag tgaggttaaa accgttatcg taatatttat
     4261 aatcgccagg tgcggtatac agttcatcaa gattaatttg tccgcgcatc accacaaaat
     4321 aatgcgccat catgacacca attaccggac caagcattcc gccgataata tcgaggaaaa
     4381 gataaatgct gtcctgattt tccattaatt tccacgggca gatcagcaag ctgataatac
     4441 tggcaatcag tacgccgttt ttataggtca gttttgtcgg tgcaatggcg gcaatctgat
     4501 aaccggctgg aataatatta ccggtcgcgt tagtggagat agttgtcatc agaataacca
     4561 gtaccgcaaa gaacgaggcg aacaggctgt cccaacgctg aacaatatcc agcacgttcc
     4621 aggtatcagc gccgtagtga atactggctc cggcaataat acataccccg gcgaccgcaa
     4681 acagaatata ggccacaact aaacccagcg tttgccccag cgcctgctca cgaaacgagt
     4741 gggcgttttg cgtaaagtcg gatgcgctca ccgccggtgc cgcccagacc gcaactaccg
     4801 cgttaatcac caccaggaac aggaagccac cgttttctgc tttctgaata ccgctcggaa
     4861 tgtagtcaaa gattggaccg atcccgacca gtgaaatcgc ccaaatcgcc ataccgccga
     4921 aaacgatata gatgcacggg ttaagaatgg cagtgaattt atttaaaact ttgccaccgc
     4981 caaaacctat accaacgttg accagccaga agattaagaa agtaattaag cccggtagag
     5041 aaaggcctaa cagagtgaaa tcaccaccga gagttaaaaa tcccggccag attttgccaa
     5101 tcagaatcaa gcaggccagt gaccccgcgt aacattgcaa accaaaccac atgatggcgg
     5161 caattccgcc ccttaataat ccgggaaaca gtgcaccacg tacaccgtaa gaagcacgca
     5221 ggatcatggc aaaaggcaca ccgtatttac tgcccgcagc accgtttaat accattaccg
     5281 cggcaatgaa aaaggcgctg aggataattg ccagcataat actaaaggta gacaagccga
     5341 gaataaaaaa gccgccgacc atcacataat tgggaacgtt atgaaccgaa cccatccata
     5401 aggtaaaata gttaaatgtt ttccaggtcc gctggctttg cgttttcggc aatagatctt
     5461 cgctatagcc gcgttgctgg aatagttttc tctgatgttc cataaatccc tcttaatttc
     5521 acgtatcgaa taacgttgtt ttaatctagc tatgaggaac aaataaaata aaaatattat
     5581 ttttaatact gaagcaacat ctttggctgc tttctttttt aatattgctg gccgattcat
     5641 tttctgatat cggccatttt tattgcgggt atcaggccag tttatggtta gccattaatt
     5701 ccagcgcctg cactaacgca gagtgatcca actggctgcc accgtttgcc gcacaggtat
     5761 taaataactc ctggcaggtc gcagtgtttg gcaggttcag cgcaagtgct ttcgcacttt
     5821 gcagtgccag gttgagatct ttctggtgca gagcgatttt gaagcccgga ttaaaggtgc
     5881 gtttaatcat acgctcgcca tgaacttcca gaatacgtga ggaagcaaag ccgcccatca
     5941 gcgcctggcg cacacgtacc gggtccgcac cggcttttga agcaaatagc agggcttcag
     6001 aaaccgcttc aatattgagc gccacgataa tctgatttgc cactttgcag gtttgaccat
     6061 cgccgttacc gcccacgagg gtgatatttt taccgagcag ttcaaacagc ggtttaacac
     6121 gttcaaatac cgcttcatca ccgccaacca taatcgacaa cgtcccttca cgcgcaccga
     6181 tttcaccgcc ggagactggc gcatcgagat aatcgccgcc cagttcattc acctgacgag
     6241 cgaaacgctt agtttcaatc ggggaaatgg agctcatatc aacaatggtt ttgcccttca
     6301 gcgaggcttt ggtacaacca ttttcaccga acagaacttc ttcaacctga ggtgtgtccg
     6361 gcaccataat aaaaatgatg tccgatgctt ccgttacctg gcgagcagtt tcaacactga
     6421 cggcacccag tgacagtaat tcatcagcaa ccggtccaat ggtcgtgaca tgtaattgat
     6481 gaccggcacg cgccagatta atggccatcg gtgtacccat aatgcctaag ccaataaatc
     6541 ccagtttcat aattaacctc ttttaaattt cgcttttcct gaattcagac aacacgatcc
     6601 cctgccaact tagcggccta aaaaagcatt gtctgaatag cgttacgttt taacggtacg
     6661 gatccatcca gcgtaaaccg gcttccgtgg tggtttgggg tttatattca cacccaaccc
     6721 aaccgttgta gtcagaattt tcgattacct taaagagata atcataatta atttctccgg
     6781 ttcccggttc gccgcgatgc ggattatcgg caatttgcag gtgaccaatt ttatcagccc
     6841 actgagtcat ggtgttggtt aattcacctt ccatccgctg catgtgataa atgtcatact
     6901 gaattttcaa attgcagcaa ccaacatcat caatcaattt cagcgcctgc cgagttccgg
     6961 tgagatggaa accaggaata tcaaaatggt taataggttc aatcagtaat aaaatatctt
     7021 ctttcatcag catattcgcg gcataacgca ggttttctac aagcgttgcg tgaatctgtt
     7081 cactgctgaa accagccggc gttttaccga ccagacagtt aattttttta ttacccagcg
     7141 cacgggcata acgaatcgct gctgctacgc catcccgaaa ctcttcttca cggccaggaa
     7201 tacaggcaat accgcgctcc cccgccgccc agtcacccgc cggtaaattg tgcagcgtat
     7261 gttcgagttt attactcgcc agcacatgtt ttaattcttc aatgtcgtag tcataaggaa
     7321 acataaattc aacgccgcga aaaccacact gcgcagcttt ctcaaaacgg gcgagaaaat
     7381 catattctcc aaataacatc gataaattag cagagaaacg taacattatt tatctccctt
     7441 attcatagtg catgaagcag gtttcagtcg gtgcgtccgc tgcgttatcg gcgatatctt
     7501 caaattccat gacgttatcc agttcgctgc ccatcgaaat attggtcaca cgctcgagaa
     7561 taacttccac gactaccggt acccgatatt gcgccattaa gactttcgcc tgttcaaagg
     7621 ctggcgcaat atcttccggt ttgaagaccc gaatagcttt acaacctaaa ccttccgcta
     7681 cttttacgtg gtcaacaccg tagccattca cttcactgga gttgatattc tcgaaagcga
     7741 gttgcacgca gtagtccatg tcaaaagcgc gttgtgactg acgaatcagg ccgagatagg
     7801 cgttattcac cagcacatgg atgtacggaa tgttgaactg cgcgccaacg gctaactctt
     7861 caatcaggaa ctggaagtca aagtcgccag aaatcgccac cacattgcgt ttcggatcag
     7921 cggcacaaac ccccagcgcc gccggaatcg tccagcctaa cggaccagcc tgaccacagt
     7981 tgatccagtg gcggtcttta aagacatgca gcatttgtgc cgcagcgatt tgtgacagac
     8041 caatggtggt gacatagcaa acatcgcgac caaaggcttt gttcatctct tcatacacgc
     8101 gctgcggttt caccggcacg ttgtcgaaat gggttttgcg cagcaaagtg cgtttgcgct
     8161 gctggcagtc ggcgacccat tctttacgac acggcagacg acccgctttt tgcatctcct
     8221 gcgccacttc aaccagcagt gtcagcgccg ctttagcatc agagacaatg ccgagatccg
     8281 gacacagcac gcgaccaatt tgcgtcggct caatatcgat atgaacgatt ttgcgccctt
     8341 cggtgtattt ctctaccgaa ccggtatgac ggttagcaaa acggttaccg ataccaaaca
     8401 ccatgtcgga cgccagcaac gttgcgttac cgtaacgatg cgcggtttgc agacccacca
     8461 tcccggccat cagttcatga tcgtccggga tacagcccca gcccatcagc gttgggatca
     8521 ccggaacgct ggtcagttca gcaaactgtt gtaacagtgc agctgcgtca gcattaatta
     8581 ccccgccccc ggcaacaatc accggacgtt cggcctggat taacatttct acagcttttt
     8641 cgatctgcat acggctggca gcaggtttgt agaccggcag cggttcgtac atgtcaggat
     8701 caaactcgat ttccgcaacc tgaacgtcga acggtaaatc caccagtacc ggacccggac
     8761 gaccagaacg catcaggtga aatgcctgtt gcagcacgcg aggcaccagc gccgcttcac
     8821 gaactgtaac cgccattttg ctgaccggtt tagcaattgc ttcaatatct acggcctgaa
     8881 aatcttcttt atgcagacgg gcgcgcggtg cctggccggt aatgcacaga ataggaatgg
     8941 aatcagcaga agcggaatag agcgcggtga tcatgtccgt gcccgcagga ccggaagtcc
     9001 ccagacatac gccgatattt cctgccgttg cgcgggtata accttccgcc atgtgcgaag
     9061 caccttccac atgacgcgcc agaatgtgac gaataccgcc gtgcttacgc atcgctgagt
     9121 agaacggatt gattgcagct cccggaacac cgaaggcggt ggtgatacct tctttctcca
     9181 gcacatacat tgccgcgtca acggctctca tttttgccat tttattccta cctctattta
     9241 ttggaaaatt tttccaactt tcaaattttg tcaaaaaaac ccgctccgca gagcgggatg
     9301 cgacagacat tatggatgtg ctttcagtcc caacgccgtg ctgatatcgc gggcggtgtc
     9361 tctgaccagc tcaccctgac tgacaaaacg atcttctgtc agtcttgatg aaggcccgga
     9421 gatggagata gcggcaacaa cactaccgac atcatcgtaa attgctgaag ctatgcaatt
     9481 cagacctaca acatgctctt ctttatctac ggtatagccc agttcacgcg cttgttccag
     9541 gtccttcagc aaggtgggca tatccacaag cgtagttggc gtaaactgct gcaaaccggt
     9601 ttgcagaatg atgctcatca actcctcttc cgccagcgga taaagcagcg ctttgcccgc
     9661 accggaagca tgcagtggca gacgactgcc cagtggcgca cacatcctga ccatcgattt
     9721 acactctaac tgaccaatta ataccgcttc attgccgtta cggatcgcga cattgaccgt
     9781 ttcgccggaa agtaacatca ggcggcgcat aaacggcccg gcgacggaga ggacatcgcg
     9841 gttatggatg tacgccgcac cgacgttaaa gacacctaat cctatatgcc accagcctaa
     9901 ttgactgtcc tgatagacaa aatccgctgc ctgtaaaacc ttcagcaagc gaaaggtcgt
     9961 ggagagcggc aaatccagat tgagagaaat atcgctaacc gacgaacttc ccccactttt
    10021 ttccaaatat tgcagaatcg caattccccg ctctaacgcc tgtgcgccct tctgtgccac
    10081 aggctccgcc tgtcctggcc tgccgcgccg tctaacttcc gtcataccta cgttaactcc
    10141 gacctacaag tgagcataaa caatgaacaa ttagtttatc ttatgcaagt cggttgcagg
    10201 cgtcacgcaa aacagagttc ctgttcagga atactttcaa catcacagtt gtcactgccg
    10261 ccgcgatcga tggtcagaaa atcggtgacg cgctgccagg cgaaaagtgg gtgatgccag
    10321 acgttacgat ggtaattcac tccctgttcg ccgttggtga taaacgcccg cagcgttgac
    10381 aggtctggtt tgtcgtcacc taacgccacg accaccacaa acacctcacc tttcatcggg
    10441 ataaaggcct gagtacccag cggatgacgt tcgagttcgt gaatggtcag cggcagattc
    10501 gccggttgcg cgcggttaat gctgataagc gtacggtctt gctcaagaat ctcaaccagc
    10561 gccaaatcgt ggtaacgctc caccaggcca ttgttaatat ggaaaaaatc ccgttgctgc
    10621 gtttcgatta cgtcgccata agcactaaag gcttcctgac ttaacggtaa tacctgaagt
    10681 ttcatctttt tgtccttgta acataattcc gttcaaccac cgccgaccgg tcagcttggt
    10741 ttcaataaca ggcaatctaa aaccttaaga gaacaacatc aatacaaaag ttggaatatt
    10801 tttccaaaac tagatttagt ccacaaattg aacagggtta tcatgtaaaa atactgataa
    10861 atcagaggat aagaaaatca cgcaacttgc ggatttaaaa ggggatacct gaagatgttc
    10921 gatccagaaa ccttgcggac tttcattgcg gttgctgaaa caggaggttt ttcaaaagcg
    10981 gcagaacgat tatgtaaaac cacggcgacg atcagttatc gcattaaact tctggaagag
    11041 aataccggag tagcgctgtt tttccgtacg actcgcagcg tgacgttgac agcggctggc
    11101 gagcatctac tttcccaggc cagagactgg ctgagctggc tggaaagtat gccaagcgag
    11161 ctgcaacagg tgaatgatgg cgtggaacgc caggtgaata ttgtcatcaa caacctgctc
    11221 tacaaccccc aggccgtcgc ccagttgctg gcgtggctga atgagcgtta cccctttacc
    11281 cagtttcaca tctcccgaca aatctatatg ggcgtctggg actcgctatt gtacgaaggt
    11341 ttttcgctgg ctatcggcgt cacgggaact gaggcgctgg caaatacctt tagtcttgat
    11401 cccttaggat cggtgcaatg gcgctttgtc atggcggcgg atcatccgct ggcgaacgtt
    11461 gaagagccgc taacagaagc gcagttgcgg cgctttccgg cggtcaatat tgaagacagc
    11521 gcccgcacct taaccaaacg cgtcgcctgg cgattgccag ggcaaaaaga gattattgtt
    11581 cccgatatgg aaacgaaaat cgccgcccat ctggcgggcg ttggcattgg ttttttgcca
    11641 aaatcgcttt gccagtcaat gatcgataat caacaactgg tcagccgggt aatcccaacg
    11701 atgcgccctc cttcgccatt gagtctggca tggcgcaaat ttggcagcgg caaagcggta
    11761 gaagatattg tgaccttgtt tacccagcgc aggccggaaa tcagcggatt tttagaaatt
    11821 ttcggcaacc cacgcagtta aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc
    11881 gcaagctgat ttgaggttca cttttaacta tgcaagagag acacacggaa caggactatc
    11941 gtgccctgct gattgctgat acgcccatta ttgatgttcg cgcccctatc gagtttgagc
    12001 aaggcgcaat gcccgccgct atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg
    12061 ttggcacctg ctataaacag caaggctcag acgcagcgct ggcgctggga cataaactgg
    12121 ttgcgggtga aattcgtcag cagcgcatgg acgcctggcg ggcagcgtgc ctgcaaaatc
    12181 cgcaaggtat tctctgctgc gcccgtggcg gtcagcgctc acatattgtg caaagctggt
    12241 tgcatgcagc gggaattgat tatccgctgg tggaaggcgg ttataaggca ctgcgccaga
    12301 ccgcgattca ggcgactatt gaactggcac aaaaaccgat agtgctgatt ggcggttgta
    12361 ccggcagcgg taaaacgctg ttagtgcagc aacagccgaa cggtgttgat ctggaagggt
    12421 tggcgcgtca tcgcggttcg gcgtttggtc gcacgttaca accacaactt agccaggcga
    12481 gttttgaaaa cctgctggct gccgaaatgc taaaaaccga cgcccgtcag aatttgcgcc
    12541 tgtgggtgct ggaagacgaa agccggatga tcggttcgaa tcacctgccg gaatgtctgc
    12601 gcgagcgaat gactcaggcg gcgattgcgg tggtagaaga tccgtttgag atccgtcttg
    12661 aacgcctgaa cgaagagtat ttcttgcgta tgcatcatga ttttacccac gcttatggcg
    12721 acgaacaggg ctggcaggaa tattgcgaat acctgcatca cggactttcg gcgattaagc
    12781 gtcggctggg gttacagcgc tataacgaac tcgctgccca gctggatacg gcgctgacaa
    12841 cgcaactcac caccggcagc accgacggtc atctggcctg gctggtgccg ttacttaaag
    12901 aatattacga cccaatgtat cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc
    12961 gcggtgagtg ggcggaagtg gcggaatggg ttaaggcgca gtgatgggcg gtattctgaa
    13021 acggcgattg tcgtgagaac agaatggttg caccagaata cagatataaa aaaaccgaca
    13081 tgacagtcgg tttttttatt ttttccgccc aagacttggt ggttccgggc agaaatgaaa
    13141 cagtcctcat ctagcaagtg aattataagt caggatgcct acatattcaa acagctatgt
    13201 tggcgataca atcccgacgc tagtagtgat actgcctcaa gggattaccg cagtgcggaa
    13261 aagtactttt aagtcctggt caggatacag gtggtgctga ttctggtgct ctaatttgga
    13321 gagaggcagt cctcatctag cacatgaaga aaatcatagt tacaggcaac gtaagggtaa
    13381 gctgtgattg tctttctccc ttaagggagc aacggatgaa aacttttctc gataaagttc
    13441 tcatcgttct taaggcgcta atcgcgctgc tggaactgat ccgtcagttt attgaataac
    13501 tgaggaaacg tagctaaggc cgaaggttta ccaccttcag tctttaaaaa aac
//
LOCUS       DACAPB010000129        13452 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.129, whole genome shotgun sequence.
ACCESSION   DACAPB010000129 DACAPB010000000
VERSION     DACAPB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.129"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..157)
                     /locus_tag="IT904_003273"
     CDS             complement(<1..157)
                     /locus_tag="IT904_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3193012.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKE"
     gene            complement(546..1829)
                     /gene="gltA"
                     /locus_tag="IT904_003274"
     CDS             complement(546..1829)
                     /gene="gltA"
                     /locus_tag="IT904_003274"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAP3193013.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(2035..2142)
                     /locus_tag="IT904_003275"
     CDS             complement(2035..2142)
                     /locus_tag="IT904_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193014.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2523..2927
                     /gene="sdhC"
                     /locus_tag="IT904_003276"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2523..2927
                     /gene="sdhC"
                     /locus_tag="IT904_003276"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP3193015.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2921..3268
                     /gene="sdhD"
                     /locus_tag="IT904_003277"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2921..3268
                     /gene="sdhD"
                     /locus_tag="IT904_003277"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAP3193016.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            3268..5034
                     /gene="sdhA"
                     /locus_tag="IT904_003278"
     CDS             3268..5034
                     /gene="sdhA"
                     /locus_tag="IT904_003278"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAP3193017.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            5050..5766
                     /locus_tag="IT904_003279"
     CDS             5050..5766
                     /locus_tag="IT904_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAP3193018.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5967..8768
                     /gene="sucA"
                     /locus_tag="IT904_003280"
     CDS             5967..8768
                     /gene="sucA"
                     /locus_tag="IT904_003280"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAP3193019.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8783..10000
                     /gene="odhB"
                     /locus_tag="IT904_003281"
     CDS             8783..10000
                     /gene="odhB"
                     /locus_tag="IT904_003281"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAP3193020.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSTGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            10275..11441
                     /gene="sucC"
                     /locus_tag="IT904_003282"
     CDS             10275..11441
                     /gene="sucC"
                     /locus_tag="IT904_003282"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAP3193021.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11441..12310
                     /gene="sucD"
                     /locus_tag="IT904_003283"
     CDS             11441..12310
                     /gene="sucD"
                     /locus_tag="IT904_003283"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAP3193022.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12414..13136)
                     /gene="mngR"
                     /locus_tag="IT904_003284"
     CDS             complement(12414..13136)
                     /gene="mngR"
                     /locus_tag="IT904_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAP3193023.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIERSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            13245..>13452
                     /locus_tag="IT904_003285"
     CDS             13245..>13452
                     /locus_tag="IT904_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193024.1"
                     /translation="MVLFYRARWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHVLTQRLAALGKISSTEQFLEEVY"
BASE COUNT         3276 a         3372 c         3591 g         3213 t
ORIGIN      
        1 cctctttatc aatatcatcc tgatgtattt cacacggtgc ttcaataact tcacctttaa
       61 agtgaatttc accggatcct tcatcagccg cataaacagg tgctgtgaac agcagagata
      121 cggccagtgc ggccaatgtt ttttgtcctt taaacataac ggagtccttt aaataatgag
      181 atagaatgtt gcgtggcaag aatatattaa ctggcagttg aatgtatttg gttttattta
      241 acaatattaa aaatcttgtt atataaagaa tggaataatt gagtgcataa atttattgtt
      301 taatgagatg aaggatgggg tgggatggat atgcataatt ttcattgggt taacaatgct
      361 accagcagga aagtataaaa ataaacttat cattctgctc agtatgtgac ttgtcacatt
      421 ttctcacctt attaatgggt atttgtatct ggtttaattt ttacaattta ttatgttcag
      481 ccatataaaa agaacccgct ctattaaagg cgggtccgga aagtaaacgg cttagcaatc
      541 aatcattaac gcttgatatc gcttttaaag tcgcgttttt catatcctgt atacagctga
      601 cgcggacggg caatcttcat accgtcactg tgcatttcgc tccagtgggc gatccagcca
      661 acggtacgtg ccattgcgaa aatgacggtg aacatggaag acggaatacc catcgctttc
      721 aggatgatac cagagtagaa atcgacgttc gggtacagtt tcttctcgat aaagtacggg
      781 tcgttcagcg cgatgttttc cagctccata gccacttcca gcaggtcatc tttggtgccc
      841 agctctttca gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg gtcgtaattt
      901 ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat catttttatc tttcgcacga
      961 cgaacaaatt ccggaatgtg tttaacggag ctaatttctt ccagcatttt cagcgccgct
     1021 tcgttagcac caccgtgcgc aggtccccac agtgaagcaa tacctgctgc gatacaggca
     1081 aacgggttcg cacccgaaga gccagcggta cgcacggtgg aggtagaggc gttctgttca
     1141 tggtcagcgt gcaggatcag aatacggtcc atagcacgtt ccagaatcgg attaacttca
     1201 tacggttcgc acggcgtgga gaacatcata ttcaggaagt taccggcata ggagagatcg
     1261 ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc ggccatggtc
     1321 ggcattttcg acagcaggcg gaacgcggca atttcacgat gacgaggatt gttaacatcc
     1381 agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac cacacatgac tgccattgga
     1441 tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct gctcgtggat catggtatga
     1501 cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag tcggtttttc accattcagc
     1561 aggatgtaac aaacttccag gtagttagaa tcggtcgcca gctgatcgat cgggaaaccg
     1621 cggtgcagca aaataccttc atcaccatca ataaaagtaa ttttagattc gcaggatgcg
     1681 gttgaagtga agcctgggtc aaaggtgaac acaccttttg aaccgagagt acggatatca
     1741 ataacatctt gacccagcgt gcctttcagc acatccagtt caacagctgt gtccccgttg
     1801 agggtgagtt ttgcttttgt atcagccatt taaggtctcc ttagcgcctt attacgtaag
     1861 actgccggaa cttaaatttg ccttcgcaca tcaacctggc tttacccgtt tgttatttgg
     1921 ctcgccgctc tgtgaaagag gggaaaacct gggtacagag ctctgggcgc ttgcaggtaa
     1981 aggatccatt gatgacgaat aaatggcgaa tcaagtactt agcaatccga attattaaac
     2041 ttgtctacca ctaataactg tcccgaatga attggtcaat acttccacac tgttacataa
     2101 gttaatctta ggtgaaatac cgacttcata acttttacgc attatatgct tttcctgata
     2161 atgtttgtaa caactttgtt gaatgattgt caaattagat gattaaaaat taaataaatg
     2221 ttgttatcgt gacctggatc actgttcagg ataaaacccg acaaactata tgtaggttaa
     2281 ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc tccggaacac cctgcaatcc
     2341 cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg
     2401 cgcagttata gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg aaatcccgac
     2461 gtctccaggt aacagaaagt taacctctgt gcccgtagtc cccagggaat aataagaaca
     2521 gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca aagacctgtt aatctggacc
     2581 tacagaccat ccggttcccc atcacggcga tagcgtccat tctccatcgc gtttccggtg
     2641 tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct gggtaccagc ctctcttccc
     2701 ctgaaggttt cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt
     2761 ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg
     2821 attttggcta tctggaagaa acattcgaag cgggtaaacg ctccgccaaa atctcctttg
     2881 ttattactgt cgtgctttca cttctcgcag gagtcctcgt atggtaagca acgcctccgc
     2941 attaggacgc aatggcgtac atgatttcat cctcgttcgc gctaccgcta tcgtcctgac
     3001 gctctacatc atttatatgg tcggtttttt cgctaccagt ggcgagctga catatgaagt
     3061 ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc accctgctgg cgctgttttc
     3121 tatcttgatc catgcctgga tcggcatgtg gcaggtgttg accgactacg ttaaaccgct
     3181 ggccttgcgc ctgatgctgc aactggtgat tgtcgttgct ctggtggttt acgtgattta
     3241 tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt
     3301 gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc aaatttccca gagcggtcaa
     3361 acctgtgcgc tgctctctaa agtcttcccg acccgttccc ataccgtttc tgcgcaaggc
     3421 ggcattaccg ttgcgctggg taatacccat gaagataact gggaatggca tatgtacgac
     3481 accgtaaaag gttcggacta tatcggtgac caggacgcga ttgaatatat gtgtaaaacc
     3541 gggccggaag cgattctgga actcgaacac atgggcctgc cgttctcgcg tcttgatgat
     3601 ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg
     3661 gcacgcactg cggcagcagc tgaccgtacc ggtcacgcac tgttgcatac cctttatcag
     3721 cagaacctga aaaaccacac cactattttc tccgagtggt atgcgctgga tctggtgaaa
     3781 aaccaggatg gcgcggtggt gggttgtact gcactgtgca tcgaaactgg tgaagtggtt
     3841 tatttcaaag cccgcgctac cgtgctggcg actggcgggg cagggcgtat ttatcagtcc
     3901 accactaacg cccacattaa cactggcgac ggcgtcggca tggctatccg cgcaggcgta
     3961 ccggtgcagg atatggaaat gtggcagttc cacccgaccg gtattgccgg tgcgggcgta
     4021 ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc
     4081 tttatggagc gttatgcgcc gaacgccaaa gacctggcgg gccgtgacgt ggtggcgcgt
     4141 tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg gccacatgcg
     4201 aaactgaaac tcgaccacct gggtaaagaa gttctcgaat cccgtctgcc gggtattctg
     4261 gaactctccc gtaccttcgc tcacgttgat ccggtgaaag agccgattcc ggttatccca
     4321 acctgtcact acatgatggg tggtattccg accaaagtga ccggtcaggc gctgacggtg
     4381 aacgagaaag gcgaagatgt ggttgttccg gggctgtttg ccgttggtga aatcgcttgt
     4441 gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt
     4501 ggtcgcgcag cgggtctgca tctgcaagag tctatcgccg agcagggcgc attgcgcgat
     4561 gccagcgagt ctgatgttga agcatctctg gatcgtctga accgctggaa caataatcgt
     4621 aacggtgaag atccggtggc gattcgtaaa gcgctgcaag aatgtatgca gcataacttc
     4681 tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg agcagttgaa agttatccgc
     4741 gagcgtctga aaaatgcccg tctggatgac acttccagcg agttcaacac ccagcgcgtt
     4801 gagtgcctgg aactggataa cctgatggaa acggcgtatg caacggctgt ttctgccaac
     4861 ttccgtaccg aaagccgtgg cgcgcatagc cgcttcgact tcccggatcg tgatgatgaa
     4921 aactggctgt gccactccct gtatctgcca gagtcggaat ccatgacgcg ccgaagcgtc
     4981 aacatggaac cgaaactgcg cccggcattc ccgccgaaga ttcgtactta ctaatgcgga
     5041 gacaggaaaa tgagactcga gttttcaatt tatcgctata acccggatgt tgatgatgct
     5101 ccgcgtatgc aggattacac cctggaagcg gaagaaggtc gcgacatgat gttgctggat
     5161 gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt tccgccgctc ctgccgtgaa
     5221 ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga atggtctggc ctgtattacc
     5281 ccgatttcgg cactcaacca gccgggcaag aagattgtga ttcgcccgct gccaggttta
     5341 ccggtgatcc gcgatttggt ggtagacatg ggacaattct atgcgcaata tgagaaaatt
     5401 aagccttacc tgttgaataa tggacaaaat ccgccagctc gcgagcattt acagatgcca
     5461 gagcagcgcg aaaaactcga cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc
     5521 tcttgtccgt ctttctggtg gaatcccgat aagtttatcg gcccggcagg cttgttagcg
     5581 gcatatcgtt tcctgatcga tagccgtgat accgagactg acagccgcct cgacggtttg
     5641 agcgatgcat tcagtgtatt ccgctgtcac agcatcatga actgcgtcag tgtatgtccg
     5701 aaggggctga acccgacgcg cgccatcggc catatcaagt cgatgttgtt gcaacgtaat
     5761 gcgtaattaa atgttgatgc cggggaaatc ccccggctat tcaggaaatc tttaaaaact
     5821 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacgt agacaagagc
     5881 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5941 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     6001 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     6061 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     6121 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     6181 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggtaaaag
     6241 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     6301 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6361 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga
     6421 aactcggcga gctgctggaa gccctcaagc aaacctactg cggcccgatt ggtgccgagt
     6481 atatgcacat caccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6541 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6601 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6661 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6721 gcgaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6781 gtaaaaaacc gcaagacttg ttcgacgagt tcgccggtaa acataaagaa cacctcggca
     6841 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6901 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6961 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     7021 tcaccatcca cggtgacgcc gcagtgaccg ggcagggtgt ggttcaggaa actctgaaca
     7081 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     7141 ttggtttcac cacctctaat ccgctggatg ctcgttctac accgtactgt actgatatcg
     7201 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     7261 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     7321 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7381 tgatgtatca gaaaatcaaa aaacatccga cgccgcgcaa aatctatgct gacaagctgg
     7441 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7501 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7561 cctggtcgcc gtatctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7621 agcgcctgca ggaactggct aaacgcatca gcacggtgcc ggaagcggtt gaaatgcagt
     7681 ctcgcgttgc caaaatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7741 actggggcgg cgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7801 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7861 accagtctaa cggttccact tacacgccgc tgcaacacat ccataacggc cagggcgcgt
     7921 tccgtgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7981 ccaccgcaga accacgcact ctgactatct gggaagcgca gttcggtgac ttcgccaacg
     8041 gtgcgcaggt ggttatcgac cagttcatct cctctggcga acagaaatgg ggccggatgt
     8101 gtggtctggt gatgttgctg ccgcacggtt atgaagggca ggggccggag cactcctccg
     8161 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtg tgcgtaccat
     8221 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     8281 cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     8341 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8401 agggtgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacaac
     8461 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8521 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8581 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8641 tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg
     8701 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8761 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8821 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8881 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8941 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     9001 ccttggtcgc ctgcgtgaag gcaacagcac cggtaaagaa accagcgcca aatctgaaga
     9061 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     9121 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     9181 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     9241 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     9301 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9361 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9421 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9481 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9541 cgcttctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9601 ttctacgccg cgcggcctgg tgacgccggt tctgcgtgat gtcgataccc tcggcatggc
     9661 agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgacggca agctgaccgt
     9721 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9781 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9841 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9901 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9961 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagt ttcacctgca
    10021 ctgtagaccg gataaggcat tatcgccttc tccggcaatt gaagcctgat gcgacgctga
    10081 cgcgtcttat caggcctacg ggaccaccaa tgtaggtcgg ataaggcgct agcgccgcat
    10141 ccgacaagcg atgcctgatg tgacgtttaa cgtgtcttat caggcctacg ggtgaccgac
    10201 aatgcccgga agcgatacga aatattcggt ctacggttta aaagataacg attactgaag
    10261 gatggacaga acacatgaac ttacatgaat atcaggcaaa acaacttttt gcccgctatg
    10321 gcttaccagc accggtgggt tatgcctgta ctactccgcg cgaagcagaa gaagccgctt
    10381 caaaaatcgg tgccggtccg tgggtagtga aatgtcaggt tcacgctggt ggccgcggta
    10441 aagcgggcgg tgtgaaagtt gtaaacagca aagaagacat ccgtgctttt gcagaaaact
    10501 ggctgggcaa gcgtctggta acgtatcaaa cagatgccaa tggccaaccg gttaaccaga
    10561 ttctggttga agcagcgacc gatatcgcta aagagctgta tctcggtgcc gttgttgacc
    10621 gtagttcccg tcgtgtggtc tttatggcct ccaccgaagg cggcgtggaa atcgaaaaag
    10681 tggcggaaga aactccgcac ctgatccata aagttgcgct tgatccgctg actggcccga
    10741 tgccgtatca gggacgcgag ctggcgttca aactgggtct ggaaggtaaa ctggttcagc
    10801 agttcaccaa aatcttcatg ggcctggcga ccattttcct ggagcgcgac ctggcgttga
    10861 tcgaaatcaa cccgctggtc atcaccaaac agggcgatct gatttgcctc gacggcaaac
    10921 tgggcgctga cggcaacgca ctgttccgcc agcctgatct gcgcgaaatg cgtgaccagt
    10981 cgcaggaaga tccgcgtgaa gcacaggctg cacagtggga actgaactac gttgcgctgg
    11041 acggtaacat cggttgtatg gttaacggcg caggtctggc gatgggtacg atggacatcg
    11101 ttaaactgca cggcggcgaa ccggctaact tccttgacgt tggcggcggc gcaaccaaag
    11161 aacgtgtaac cgaagcgttc aaaatcatcc tctctgacga caaagtgaaa gccgttctgg
    11221 ttaacatctt cggcggtatc gttcgttgcg acctgatcgc tgacggtatc atcggcgcgg
    11281 tagcagaagt gggtgttaac gtaccggtcg tggtacgtct ggaaggtaac aacgccgaac
    11341 tcggcgcgaa gaaactggct gacagcggcc tgaatattat tgcagcaaaa ggtctgacgg
    11401 atgcagctca gcaggttgtt gccgcagtgg aggggaaata atgtccattt taatcgataa
    11461 aaacaccaag gttatctgcc agggctttac cggtagccag gggactttcc actcagaaca
    11521 ggccattgca tacggcacta aaatggttgg tggcgtaact ccaggtaaag gcggcactac
    11581 ccacctcggc ctgccggtgt tcaacaccgt gcgtgaagct gttgctgcta ctggcgctac
    11641 cgcttctgtt atctacgtac cagcaccgtt ctgcaaagac tccattctgg aagccatcga
    11701 cgcaggcatc aaactgatta tcaccatcac tgaaggcatc ccgacgctgg atatgctgac
    11761 tgtgaaagtg aaactggacg aagcaggcgt tcgtatgatc ggcccgaact gcccaggcgt
    11821 tatcactccg ggtgaatgca aaatcggtat ccagccgggt cacattcaca aaccgggtaa
    11881 agtgggtatc gtttcccgtt ccggtacact gacctatgaa gcggttaaac agaccacgga
    11941 ttacggtttc ggtcagtcga cctgtgtcgg tatcggcggt gacccgatcc ctggctctaa
    12001 ctttatcgac attctcgaaa tgttcgaaaa agatccgcag accgaagcga tcgtgatgat
    12061 tggtgagatc ggcggtagcg ctgaagaaga agcggcagcg tacatcaaag agcacgttac
    12121 caagccagtt gtgggctaca ttgctggtgt gactgcgccg aaaggcaaac gtatgggcca
    12181 cgcgggtgcc atcattgccg gtgggaaagg gactgcggat gagaaattcg ctgctctgga
    12241 agccgcaggc gtgaaaaccg ttcgcagcct ggcggatatc ggtgaagcac tgaaaactgt
    12301 tctgaaataa atatctgtaa taagaaatag ccctcgccgc ttccctctac aggaatggcg
    12361 aagggctgtc ggtttcgaca tggttggcca tcgtatgatg gccttttttg tgcttatcgc
    12421 gatgattttc gctgcgctat cagggtaaat ttatagtcat cggtattaaa agcgttgcgg
    12481 ctatattcaa acacccgacc atcaactaaa tatccacgcg atactttttc aagaatcggc
    12541 tttgtctggc tgatattaag cagacggctc atctcttcgg ttggcatcag aggaatgatt
    12601 tcctgttcgc tacgctcgat aaccattttc ttcacttctt caataaagtg atatttcgaa
    12661 ttttccatga cctgccaggt gagatccggg aacaacgcaa gcggcatcca ggtttcttcc
    12721 agcgccattg gcttttgctt gcgatagcgc acgcgcttca catgccacac acgatcctgc
    12781 gcggtgattt gtagctgttg ctgaagaaaa tcgtcagccg gaatcacttc aaatatcagg
    12841 acttcactgt gtgtatcgac gtgacggtcc gacagttttt catcaaaact ggttaactga
    12901 aaaatatcgt aattgacccg ctcttctttg acgtaagtcc cgctgccctg aatgctttcg
    12961 aggatctgct gctcgactag ctggcgcaaa gcctgacgca ccgtaacccg gctgacgcca
    13021 aactctgttt gtaatgcaga ttcggtaggt aacgcatcgc caggtttaag ctccccacgc
    13081 gcaatttgct cacgaatgcg atcggcaatc tgccggtata agggtttgtg tcccattttt
    13141 agtatctcat taatacgaat ttagccatta tgcccgataa attcatcctg taaataatac
    13201 aaatacaata caaataattt caatcaagtg aaattgatca cataatggta ttgttttatc
    13261 gggcacgctg gcgcgactat aaaaacgatc aagtgaggat catgatgaat ctgacgactc
    13321 tgacccaccg cgatgcgttg tgtctgaatg cgcgctttac cagccgtgaa gaggccatcc
    13381 acgtgttgac tcaacgtctt gctgctctgg ggaaaatttc cagtactgag caatttctgg
    13441 aagaagtgta tc
//
LOCUS       DACAPB010000130        13192 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.130, whole genome shotgun sequence.
ACCESSION   DACAPB010000130 DACAPB010000000
VERSION     DACAPB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.130"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(75..563)
                     /gene="eco"
                     /locus_tag="IT904_003286"
     CDS             complement(75..563)
                     /gene="eco"
                     /locus_tag="IT904_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAP3193025.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDQSTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(698..862)
                     /locus_tag="IT904_003287"
     CDS             complement(698..862)
                     /locus_tag="IT904_003287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193026.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            971..1465
                     /gene="napF"
                     /locus_tag="IT904_003288"
     CDS             971..1465
                     /gene="napF"
                     /locus_tag="IT904_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAP3193027.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            1455..1718
                     /gene="napD"
                     /locus_tag="IT904_003289"
     CDS             1455..1718
                     /gene="napD"
                     /locus_tag="IT904_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAP3193028.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1715..4201
                     /gene="napA"
                     /locus_tag="IT904_003290"
     CDS             1715..4201
                     /gene="napA"
                     /locus_tag="IT904_003290"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAP3193029.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            4208..4903
                     /gene="napG"
                     /locus_tag="IT904_003291"
     CDS             4208..4903
                     /gene="napG"
                     /locus_tag="IT904_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAP3193030.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4890..5768
                     /gene="napH"
                     /locus_tag="IT904_003292"
     CDS             4890..5768
                     /gene="napH"
                     /locus_tag="IT904_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAP3193031.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSK
                     GVITVAATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCS
                     EDVFTITTRWSSGAKS"
     gene            5765..6214
                     /gene="napB"
                     /locus_tag="IT904_003293"
     CDS             5765..6214
                     /gene="napB"
                     /locus_tag="IT904_003293"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAP3193032.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            6224..6826
                     /gene="napC"
                     /locus_tag="IT904_003294"
     CDS             6224..6826
                     /gene="napC"
                     /locus_tag="IT904_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAP3193033.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6845..7462
                     /gene="ccmA"
                     /locus_tag="IT904_003295"
     CDS             6845..7462
                     /gene="ccmA"
                     /locus_tag="IT904_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAP3193034.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            7459..8121
                     /gene="ccmB"
                     /locus_tag="IT904_003296"
     CDS             7459..8121
                     /gene="ccmB"
                     /locus_tag="IT904_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAP3193035.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            8163..8900
                     /gene="ccmC"
                     /locus_tag="IT904_003297"
     CDS             8163..8900
                     /gene="ccmC"
                     /locus_tag="IT904_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAP3193036.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8897..9106
                     /gene="ccmD"
                     /locus_tag="IT904_003298"
     CDS             8897..9106
                     /gene="ccmD"
                     /locus_tag="IT904_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAP3193037.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQLRAREARLRAAQQQEAA"
     gene            9103..9582
                     /gene="ccmE"
                     /locus_tag="IT904_003299"
     CDS             9103..9582
                     /gene="ccmE"
                     /locus_tag="IT904_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAP3193038.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            9579..11522
                     /locus_tag="IT904_003300"
     CDS             9579..11522
                     /locus_tag="IT904_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP3193039.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            11519..12076
                     /gene="dsbE"
                     /locus_tag="IT904_003301"
     CDS             11519..12076
                     /gene="dsbE"
                     /locus_tag="IT904_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAP3193040.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            12073..13125
                     /locus_tag="IT904_003302"
     CDS             12073..13125
                     /locus_tag="IT904_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAP3193041.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKTDPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
BASE COUNT         2759 a         3350 c         3904 g         3179 t
ORIGIN      
        1 cggctcgggc gttggatgtc ggatgcggcg tgaccgcctt gtctgaccta cggggtgccg
       61 cacttcacgg cagtttagcg aactaccgcg ttgtcaattt tctcttccgc cttccagacg
      121 cggtacttca catctacatt gtctggcgta tacaccacga tcggcagctt gctgttgtaa
      181 cgcagcattc cagcatcgcc cagatacgcg gtgacaaatt tcttctcttt cttgccatcc
      241 gggcaggcca tcatcgtcga aaccggggaa ctgactttat caaagacata ataatcatag
      301 ccccagcctt ccagcgtttt gttttccagc ttcccgccga gacgatgcaa attgcaatcg
      361 acttccagcg tctgaccgat taacagttct actttcaggg tagattgatc ttcttgcggg
      421 gttaactgaa tcacctgacg cttcatccct ttttcagctt gtggataagg cgcgattttt
      481 tccagtggct ggacgctttc tgccgcccag gcggaagtgg tagcgaaagc ggcaaacaat
      541 actgcaggta gaatggtctt catttttttg ctttccttct tcatttatag gttttcaccg
      601 atgatgtcgc tgaaggttaa catattcatc agatttatta atttgcttta tgaataactt
      661 aaagaacatt cagacaattt tacctgctct tttagtgtta aattctaatg agagagcaaa
      721 ccagcagaaa tatctctggt gaattaatcg ctatataaat atatttataa ccatttgaaa
      781 tgtgagcaaa agcccgtttt tgccacgctt cccgccactc ttttgatcct gctacaggtt
      841 ttaccccgat cggggtatgc atctttgaca catcctttaa tatcttagcg gctataaaaa
      901 tggcttatta attatgcggt ttatttggtc gctctcaatt ttcagagcgc gttaatgatg
      961 gaaggtcaat gtgaagattg atgcatcccg tcggggcata ctcactggtc gctggcgcaa
     1021 agccagtaac ggtatccgtc cgccctggtc cggtgatgaa tctcattttc tgacccattg
     1081 cacccgctgc gacgcctgta ttaatgcttg tgaaaacaac attctgcaac gcggcgcggg
     1141 cggctatccg agcgttaatt tcaaaaataa cgagtgcagc ttctgttacg actgcgcgca
     1201 ggcttgcccc gaatcgctat tttctccgcg ccacaccagg gcctgggatt tgcagttcac
     1261 cattggggac gcgtgcctgg cgtatcagtc tgttgaatgc cgccgctgtc aggatagctg
     1321 tgagccaatg gcaattatct ttcgcccgac gctctccggg atctaccagc cgcaacttaa
     1381 tagccaactt tgcaacggat gtggagcatg tgcagccagc tgtccggtat cagccataac
     1441 tgcggagtat ctccatgcac actaactggc aagtttgcag cctggtcgtg caggccaaaa
     1501 gcgaacgaat ttcagacatc agcacccaac tgaacgcctt tcccggctgt gaagttgctg
     1561 tcagcgacgc gccgagcggt cagttgattg tggtggtgga agcagaagac agcgaaacgc
     1621 tgatccaaac cattgagtca gtacgcaacg tagagggcgt gctggcggtg tcgctggttt
     1681 atcaccagca ggaagagcaa ggtgaggaaa caccatgaaa ctcagtcgtc gtagctttat
     1741 gaaagctaac gccgttgcgg ccgctgcggc ggctgccggt ctcagcgtgc cgggcgttgc
     1801 ccgcgccgtt gttggtcagc aggaagccat caaatgggat aaagcgccgt gccgtttctg
     1861 cggtactggt tgcggcgttc tggtcggaac gcagcaggga cgtgtggtgg cctgtcaggg
     1921 cgacccggac gcaccggtta accgtggcct gaactgcatt aagggctatt tcctgcccaa
     1981 gatcatgtac ggtaaagacc gtttgacgca gccgctgctg cgtatgaaaa acggtaaata
     2041 tgacaaagaa ggcgaattta ccccaatcac ctgggatcag gccttcgatg tgatggaaga
     2101 gaaattcaaa accgccctga aagaaaaagg accggaatcg atcggtatgt tcggttctgg
     2161 tcagtggact atctgggaag gttatgccgc gtccaagctg ttcaaagcgg gcttccgttc
     2221 gaacaacatc gacccgaacg cacgtcactg tatggcgtcg gcagtagttg gctttatgcg
     2281 tacctttggt atggatgagc cgatgggctg ctatgacgac atcgagcagg ctgacgcgtt
     2341 tgtgctgtgg ggcgctaaca tggcggagat gcacccgatc ctctggtcac gcatcactaa
     2401 ccgtcgtctc tctaaccaga acgtcaccgt ggcggtgctt tctacctacc agcatcgtag
     2461 cttcgagctg gcggataacg gcatcatctt tacgccgcaa tctgacctgg tgatcctgaa
     2521 ctacatcgcc aactatatca ttcaaaacaa tgcgataaat caggacttct tcagcaagca
     2581 cgttaacctg cgcaaagggg cgacggacat cggctacggt ttacgtccga cccatccgct
     2641 ggaaaaagca gcgaagaatc cgggttctga cgcctccgaa ccgatgagct ttgaagatta
     2701 caaagccttc gttgccgagt atacgctgga aaaaactgcc gaaatgaccg gcgtgccgaa
     2761 agaccagtta gaacaactgg cgcagctgta tgccgatccg aacaagaaag tcatctccta
     2821 ctggacgatg ggcttcaacc agcatactcg tggcgtgtgg gccaacaacc tggtctacaa
     2881 cctgcacctg ctgaccggca aaatttccca gccgggttgc ggtccgttct ccctgaccgg
     2941 gcagccttcc gcgtgtggta ctgcgcgtga agtgggcacc tttgctcacc gtctgcctgc
     3001 ggacatggtg gtgactaacg agaaacaccg tgatatttgc gagaagaagt ggaatatccc
     3061 gagcggcacc attccggcga aaatcggtct gcacgcggtg gcacaagacc gtgcactgaa
     3121 agacggcaag ctgaatgttt actggaccat gtgtaccaac aacatgcagg ccgggccgaa
     3181 cattaatgaa gagcgtatgc cgggctggcg cgatccgcgc aacttcatca tcgtctccga
     3241 tccgtatccg acagtcagtg cgctggcagc cgacttgatc ctgccgaccg caatgtgggt
     3301 agagaaagag ggcgcttacg gtaacgccga acgccgtact cagttctggc gtcagcaggt
     3361 acaggcgcca ggcgaagcga aatcggatct ctggcagtta gtccagttct cccgccgctt
     3421 caaaactgaa gaagtatggc cggaagagct gctggcgaag aaaccggaac tgcgtggcaa
     3481 aacgctgtac gaagttctgt atgccacccc cgaagtgagc aaattcccgg tatccgaact
     3541 ggcggaagat cagctgaacg atgaatcccg cgagctgggc ttctatctgc aaaaagggct
     3601 gttcgaagag tacgcatggt ttggtcgcgg tcacggtcac gatctggcac cgttcgatga
     3661 ctaccacaaa gcgcgcggtc tgcgctggcc ggtggtgaac ggtaaagaaa cgcagtggcg
     3721 ttacagcgaa ggtaacgacc cgtacgtgaa agcgggcgaa ggctacaagt tctacggtaa
     3781 accggatggc aaagcggtga tcttcgcgct gccgttcgaa ccggcggcgg aagcaccgga
     3841 tgaagagtac gacctgtggc tctctaccgg acgcgtcctg gagcactggc acaccggcag
     3901 tatgactcgc cgtgtgccgg aactgcaccg cgccttcccg gaagcggtcc tgtttattca
     3961 cccgctggat gcgaaagcgc gcgatctgcg ccgtggcgac aaagtgaaag tggtttctcg
     4021 ccgtggcgaa gtgatctcga ttgttgaaac gcgcggtcgt aaccgtccgc cacagggcct
     4081 ggtgtacatg ccgttcttcg acgccgcaca gctggttaac aaactgacgc tggatgcgac
     4141 cgatccgctc tcgaaagaga cggacttcaa gaagtgcgcg gtcaaactgg agaaggtgta
     4201 agccattatg tcccggtcag cgaaacctca aaatggtcgc cgccgctttc tgcgcgatgt
     4261 tgttcgcaca gcaggcgggc tggctgccgt gggtgtggcg ctggggttac aacagcaaac
     4321 cgcacgcgca tctggcgtgc ggttgcgccc gcccggagcc ataaacgaga acgcctttgc
     4381 cagtgcctgt gtgcgttgcg gtcagtgtgt tcaggcttgc ccttacgaca ccttaaaact
     4441 ggcgacgctg gcctctggtc tgtcggcggg cacgccatat tttgtcgcac gggatattcc
     4501 ttgcgaaatg tgtgaggaca ttccgtgcgc caaagtgtgc ccgagcggtg cgctggatcg
     4561 tgagatcgaa tcgatcgacg acgcgcggat ggggctggcg gtactggtgg accaggaaaa
     4621 ctgtctcaac tttcaggggc tgcgctgcga tgtttgttat cgcgaatgcc cgaaaattga
     4681 tgaggccatc accctggagc tggagcgcaa cacgcgtacc ggtaagcacg cccgctttct
     4741 gccgacggtt cacagcgacg cctgtaccgg ttgcggtaag tgcgaaaaag tatgcgtgct
     4801 ggaacaaccg gcaatcaagg tgttaccgct gtcactggcg aaaggggagt taggtcacca
     4861 ttaccgcttc ggctggctgg aggggaacaa tggcaaatcg taaacgtgac gccgggcgcg
     4921 aggcgctgga gaaaaaaggc tggtggcgca gtcaccgttg gctggtgtta cgtcgccttt
     4981 gtcagttctt cgtgctgggg atgtttttga gcggtccatg gtttggtgtg tggatcttgc
     5041 acggcaacta cagcagtagc ctgttattcg acaccgttcc gctgaccgat ccgctgatga
     5101 cgctgcaaag tcttgccagt ggtcatctgc ctgccacggt ggcgttgacc ggtgcggtga
     5161 ttatcaccgt gctctacgcc ctggcgggaa agcgattatt ttgcagctgg gtctgcccgc
     5221 tgaacccgat taccgactta gcgaactggt tacgcaggcg ctttgacctc aatcagtctg
     5281 cgacgatccc tcgccacatt cgctacgtgc tgttggtggt cattctggtg ggatcagcct
     5341 taactggcac gctcatttgg gaatggatca accccgtttc tctgatgggg cgtagtctgg
     5401 taatgggctt cggcagcggc gcgctgctta ttctcgcgct gcttattctc gcgctgtttt
     5461 tatttgatct actggtcgtt gaacacggct ggtgcgggca catttgtccg gttggcgcgc
     5521 tgtatggcgt gctgggtagc aaaggtgtga ttaccgttgc ggccaccgat cggcagaaat
     5581 gtaaccgctg tatggattgt tttcatgttt gcccggaacc gcatgtgcta cgtgccccgg
     5641 tgctggatga acaaagcccg gtgcaggtca ccagccgcga ttgcatgacg tgcggtcgct
     5701 gcgtggatgt ctgttctgag gatgtattta caataactac acgatggagt tcgggagcga
     5761 aatcatgaaa agccatgacc tgaagaaagc gctgtgtcaa tggacggcga tgctggccct
     5821 ggtggtaagc ggcgcggttt gggcggctaa tggcgtcgat tttagccagt cgccggaagt
     5881 atccgggaca caggaagggg ccattcggat gccaaaagag caggaacgga tgccgctgaa
     5941 ctatgtgaat cagccgccaa tgatcccgca tagcgttgaa ggttatcagg taacgaccaa
     6001 taccaaccgc tgcttgcagt gccacggtgt cgaaagctat cgcaccactg gcgcgccgcg
     6061 tatcagtcct actcacttta tggacagcga cggcaaagtg ggcgcggaag tggctccgcg
     6121 tcgttatttc tgtctgcaat gtcacgtacc gcaggccgat accgcgccaa tcgtggggaa
     6181 tacctttacc ccatcaaaag gttacgggaa ataagaggtt attatgggaa attctgaccg
     6241 taagcctggt ctgattaagc gcctgtggaa atggtggcgt acccccagcc gtctggcgct
     6301 ggggacgctg ctgttgatcg gttttgttgg cggcatcgtc ttctggggtg gctttaacac
     6361 cgggatggaa aaagccaata ccgaagagtt ctgcattagc tgccacgaaa tgcgcaacac
     6421 ggtgtatcag gaatacatgg attccgtgca ctacaacaac cgtagcggcg tccgtgcgac
     6481 ctgtccggat tgtcacgttc cgcacgagtt tgtgccgaag atgatacgca agctcaaagc
     6541 aagtaaagag ctgtatggta aaatttttgg cgttattgac acgccgcaga aatttgaagc
     6601 tcatcgtctg acgatggcac agaatgagtg gcggcgcatg aaggacaata actcgcagga
     6661 gtgccgtaac tgtcacaact tcgagtatat ggatacaacc gcccagaaat cggttgccgc
     6721 gaagatgcat gaccaggcgg tgaaagatgg gcaaacctgt attgattgcc ataaagggat
     6781 agcgcacaag ctgcccgata tgcgtgaagt cgagccaggt ttttaacagg gttattgcgt
     6841 gggtatgctt gaagccagag agttactttg tgagcgggat gaacgaacct tatttagtgg
     6901 cttgtcattt acgctgaacg caggagagtg ggtacaaatc accggtagca acggcgcggg
     6961 gaagacaacg cttctccgtt tgctgacggg gttgtctcgc cctgacgcag gcgaggttct
     7021 ctggcaaggg cagcccttgc atcaggtacg cgacagctac catcaaaacc tgttatggat
     7081 aggccatcag ccggggatca aaacccggct gacggcgtta gaaaatctgc acttttatca
     7141 tcgcgatggc gataccgcac aatgtctgga agccctggcg caggccgggc ttgccggatt
     7201 cgaagatatt cctgtaaatc agctctcggc cgggcaacaa cgccgcgtcg ctttagcgcg
     7261 tctgtggctg acccgtgcca cgttatggat cctcgacgag ccttttaccg cgattgacgt
     7321 taacggcgtc gatcgtctga cccagcgtat ggcgcagcat acggagcagg gggggattgt
     7381 gattctgact acccaccagc cgctcaacgt tgctgaaagt aaaattcgcc gcatttcact
     7441 gacgcaaacg agggccgcat gatgttctgg cgcattttcc gtcttgagct gcgtgtagcg
     7501 tttcgccata gcgccgaaat cgccaacccg ctgtggttct tcctgattgt aattaccctt
     7561 tttccgctca gtatcggtcc ggagccgcaa ctgctggcgc gtattgcacc gggcattatc
     7621 tgggttgctg cgctgctttc atccttgctg gcgctggaac gactgttccg tgacgatttg
     7681 caggacggca gtcttgaaca attgatgttg ttgccgttac ccttgcccgc cgttgtgctg
     7741 gcgaaggtga tggcgcactg gatggtaacc ggtctgccgt tactcatcct ttcgccactg
     7801 gtagcaatgc tactgggaat ggatgtttat ggctggcaag tgatggcgct gacgctgctg
     7861 ctgggaacgc ctacgcttgg ctttctcggt gcaccgggtg tggcgttgac agtgggactt
     7921 aagcgcggtg gtgtgctgct cagcatactg gtgttaccgc tgactatccc attactcatc
     7981 tttgccaccg ccgcgatgga cgcggcttcc atgcatttgc ccgttgacgg gtatctggca
     8041 attttaggcg cgttgctggc aggcaccgcg acattaagtc cttttgcgac ggcggcagcg
     8101 ttacgaatca gcattcaata acgctggatt actgattttt gttcgagtct ggtatcgaaa
     8161 ctatgtggaa aacactgcat caactggcga tcccaccacg gctgtatcaa atctgtggct
     8221 ggtttatacc gtggctggca attgccagtg tggtcgtgct taccgtcggc tggatctggg
     8281 gattcggctt tgctccggct gattatcagc agggaaatag ctaccgcatt atctacctgc
     8341 atgtgcctgc ggcgatctgg tcgatgggca tttatgcatc aatggcagtg gcagcgttta
     8401 ttggccttgt ctggcagatg aaaatggcca acctggcggt ggcggcgatg gcccccattg
     8461 gtgccgtgtt tacctttatt gccctggtta ccggctctgc atggggaaaa ccgatgtggg
     8521 gcacctggtg ggtatgggat gcacgtctga cttctgaact ggtgctgctg tttttgtatg
     8581 tgggtgtgat tgccctgtgg cacgccttcg acgaccgccg tctggcgggc cgtgcggcag
     8641 gtatcctggt gctgattggc gtggtgaatc tgccgattat tcattactcc gtggagtggt
     8701 ggaacaccct gcatcaggga tcaacgcgga tgcagcaaag tatcgatccg gcgatgcgtt
     8761 cgccgctgcg ctggtcgatt tttggcttcc tgctcctgtc tgccacgctg acgctgatgc
     8821 ggatgcgtaa tttgattttg ctgatggaaa aacgccgtcc gtgggtgagt gaactgatac
     8881 tgaaaagagg ccgtaaatga cccctgcatt tgcttcctgg aatgaatttt tcgcaatggg
     8941 cggttacgcc ttttttgtct ggctggcggt ggtgatgacc gttattccgc tggtggtttt
     9001 ggtcgtgcac tcggtgatgc aacatcgcgc aattctgcgt ggtgtggcgc aactgcgggc
     9061 gcgtgaggcg cgtttacgtg ctgcgcaaca gcaggaggct gcatgaatat tcgccgtaaa
     9121 aaccgcttgt ggattgcctg tgccgtgttg gcagggctgg cgctgactat cggtctggtg
     9181 ctatatgcgc tgcgctcgaa tatcgatctc ttttatacgc cgggggaaat tctctacggc
     9241 aagcgtgaaa ctcagcaaat gccggaagtc ggtcagcgtc tgcgcgttgg cgggatggtg
     9301 atgccgggta gcgtgcagcg cgatcccaat tcgctaaaag taactttcac catttacgac
     9361 gctgaaggtt cagtagatgt ctcttacgaa ggcattttgc cggatctgtt ccgtgaaggg
     9421 cagggcgttg tggtgcaggg cgaactggaa aaaggcaatc atatcctcgc aaaagaagtg
     9481 ctggcgaaac acgacgaaaa ctacacgccg ccagaagttg agaaagcgat ggaagccaat
     9541 caccgtcgcc cggcgagtgt ttataaggac ccagcatcat gatgccggaa attggtaacg
     9601 ggctgctgtg tctggcgcta ggaattgcgc tgctgctgtc cgtgtatccg ctatggggcg
     9661 tagcgcgcgg agatgcgcgc atgatggcgt cttcccgctt gtttgcctgg ctgctgttta
     9721 tgtctgtggc tggcgcattt ctggtactgg tcaatgcctt cgtggtcaac gacttcaccg
     9781 tcacctatgt tgccagcaac tccaataccc agcttccggt gtggtatcgc gtggcggcta
     9841 cctggggcgc gcatgaaggc tcgctcctgc tgtgggtgct gctgatgagc ggctggacct
     9901 ttgcggtagc gatttttagt cagcgtattc cgctggatat tgtggcccgc gtactggcga
     9961 taatggggat ggtcagcgtc ggctttttgc tgttcattct ctttacctct aacccgttct
    10021 cacgcacgtt gccgaacttc ccgattgaag ggcgcgatct taacccgctg ttacaggatc
    10081 cggggctgat cttccatccg cctctgctct atatggggta cgtggggttc tcggtggcgt
    10141 ttgcttttgc cattgcttct ttgctgagcg ggcgtctgga cagcacttat gcgcgtttta
    10201 ctcgcccgtg gacgctggcg gcgtggattt tcctgacgct cggcattgtg ctcggttccg
    10261 catgggccta ttacgaactc ggctggggcg gctggtggtt ctgggatccg gtagaaaacg
    10321 cctcgtttat gccgtggctg gtggggactg cgctgatgca ctcactggcg gtcactgaac
    10381 aacgcgccag tttcaaagcg tggacattac tgctggcaat cagtgccttc tcgttgtgtc
    10441 tgctggggac cttcctcgtg cgttccggcg tgctggtatc ggtacacgcg tttgcgtctg
    10501 atccggcgcg cggtatgttt atcctcgcct ttatggtgct ggtgattggc ggttcgctgc
    10561 tgctgtttgc cgcgcgtgga cacaaagttc gctcacgcgt aaacaatgcg ctgtggtcgc
    10621 gggaatcttt gctgttagcg aacaatgttt tgctggtcgc tgcgatgctg gtggtgttgc
    10681 tggggacgct gctgccgttg gtgcataagc aactgggact gggcagtatt tcgattggcg
    10741 aaccgttctt caacaccatg tttacctggc tgatggtgcc gtttgcgcta ctgcttggtg
    10801 tcggtcctct ggtgcgctgg gggcgggatc gcccgcgtaa gatccgcaat ttattgatta
    10861 tcgccttcat ctctacgctg gtgctgtcgc tgctgttgcc gtggctgttc gaaagcaaag
    10921 ttgtggcgat gacggtgctc ggcctggcaa tggcctgctg gattgcggtg ctggcaattg
    10981 cggaagctgc gctacgtatt tcacgcggca cgaaaaccac cttcagttat tgggggatgg
    11041 tggcggctca ccttgggctg gcagtgacaa ttgttggcat tgcctttagc cagaactata
    11101 gcgttgagcg tgatgtgcgc atgaagtccg gcgatagcgt cgatattcat gaatatcgct
    11161 tcaccttccg tgatgtcaaa gaggtgactg gcccgaactg gcgtggcggt gtggcgacta
    11221 tcggcgtaac gcgcgatggc aagccggaaa cggtgctgta tgcggaaaaa cgttattaca
    11281 acactgccgg gtcgatgatg accgaagcgg caattgacgg cggcatcacg cgtgacctgt
    11341 acgccgcgct cggtgaagag ctggaaaacg gcgcgtgggc cgtgcgtctt tactacaaac
    11401 catttgttcg ctggatttgg gcgggcgggc tgatgatggc gttgggcgga ctgctgtgtc
    11461 tgtttgatcc tcgctatcgt aagcgcgtga gtccgcaaaa aactgcgccg gaggccgtat
    11521 gaagcgcaaa gtattgttaa ttccgttgat tatcttcctg gcgattgccg cggcgctgct
    11581 gtggcagctg gcgcgtaatg ccgaagggga tgatccgacc aatctggaat cggcgctcat
    11641 tggcaagcct gtgccgaagt ttcgtctcga atcactggac aatccggggc agttttatca
    11701 ggcggatgtg ctgactcagg gcaaaccagt actgcttaac gtctgggcga cctggtgtcc
    11761 gacctgccgt gcggaacatc aatatctgaa tcagctttca gcgcagggca ttcgcgtggt
    11821 cggcatgaac tataaagacg atcgccagaa ggcaatcagc tggctgaaag agctgggcaa
    11881 tccttacgcg ctaagcctgt ttgatggcga cggcatgtta gggctggatc tcggtgtcta
    11941 tggcgcgcca gaaaccttcc ttattgacgg caacggtatc attcgctatc gccatgcggg
    12001 cgatctcaat cctcgcgtct gggaagaaga gatcaagccg ctgtgggaga aatacagtaa
    12061 ggaggccgca caatgaggtt tttattgggc gtgctgatgc tgatgatctc cggctcagca
    12121 ctggcgacca tcgacgtgtt gcagtttaaa gatgaagcac aggaacaaca gttccgtcag
    12181 ctcactgaag aactgcgctg cccgaaatgc cagaacaaca gcattgccga ttccaactcg
    12241 atgattgcca ccgacctgcg tcagaaagtg tatgaactga tgcaggaagg taaaagtaaa
    12301 aaagagattg tcgattatat ggtggcgcgt tacggcaact tcgtcactta cgatccgccg
    12361 ttaacgccgc tgaccgtgct gctgtgggtg cttccggtag tggctattgg cattggcggt
    12421 tgggtcatat acgcccgctc gcggcgtcgg gtacgcgtag tgccggaagc gtttcctgaa
    12481 caaagcgtgc aggaaggtaa gcgtgccgga tatattgttt atctgccggg tattgtggtg
    12541 gcgttaattg tggctggcgt cagctactac cagactggca attatcagca ggtgaaaatc
    12601 tggcagcagg ccacggcaca ggctccggcg ttgctggaca gggcgctgga tccgaaaacc
    12661 gatccgctca acgaagaaga gatgtcgcgt cttgcgctgg ggatgcgtac tcaactgcaa
    12721 aaaaatccgg gagatataga aggctggatt atgttgggcc gcgttggcat ggcgctgggt
    12781 aacgctagta tcgccaccga tgcatacgct actgcgtatc gcctcgatcc gaaaaacagc
    12841 gatgccgcac tgggttatgc tgaagcgttg acacgttcat ctgatcccaa cgacaaccgc
    12901 ctcggtggtg aactgctgcg ccagttggtg agaagcgacc acagcaatat ccgtgtgtta
    12961 agcatgtatg cgtttaatgc ctttgagcag cagcgatttg gcgaagccgt tgccgcgtgg
    13021 gagatgatgt tgaaactctt acctgccaac gatactcgcc gtgcggtgat tgaacgtagt
    13081 atcgcgcagg cgatgcaaca tttgtcgccg caggagagta aataaaaaga ggcccgatgg
    13141 tgatgccatc gggctagact gttgacaaag tgcgcttagt ttatgccgga tg
//
LOCUS       DACAPB010000131        12988 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.131, whole genome shotgun sequence.
ACCESSION   DACAPB010000131 DACAPB010000000
VERSION     DACAPB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.131"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..370
                     /gene="tatB"
                     /locus_tag="IT904_003303"
     CDS             <1..370
                     /gene="tatB"
                     /locus_tag="IT904_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAP3193042.1"
                     /translation="LTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMK
                     RSYVANDPEKASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVK
                     PAADAEPKTAAPSPSSSDKP"
     gene            373..1149
                     /gene="tatC"
                     /locus_tag="IT904_003304"
     CDS             373..1149
                     /gene="tatC"
                     /locus_tag="IT904_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAP3193043.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            1191..1973
                     /gene="tatD"
                     /locus_tag="IT904_003305"
     CDS             1191..1973
                     /gene="tatD"
                     /locus_tag="IT904_003305"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAP3193044.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVACAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(1970..2458)
                     /gene="rfaH"
                     /locus_tag="IT904_003306"
     CDS             complement(1970..2458)
                     /gene="rfaH"
                     /locus_tag="IT904_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAP3193045.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            2625..4118
                     /gene="ubiD"
                     /locus_tag="IT904_003307"
     CDS             2625..4118
                     /gene="ubiD"
                     /locus_tag="IT904_003307"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAP3193046.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            4164..4865
                     /gene="fre"
                     /locus_tag="IT904_003308"
     CDS             4164..4865
                     /gene="fre"
                     /locus_tag="IT904_003308"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAP3193047.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(5148..6311)
                     /gene="fadA"
                     /locus_tag="IT904_003309"
     CDS             complement(5148..6311)
                     /gene="fadA"
                     /locus_tag="IT904_003309"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAP3193048.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(6321..8510)
                     /gene="fadB"
                     /locus_tag="IT904_003310"
     CDS             complement(6321..8510)
                     /gene="fadB"
                     /locus_tag="IT904_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAP3193049.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            8700..10031
                     /gene="pepQ"
                     /locus_tag="IT904_003311"
     CDS             8700..10031
                     /gene="pepQ"
                     /locus_tag="IT904_003311"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAP3193050.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            10031..10645
                     /locus_tag="IT904_003312"
     CDS             10031..10645
                     /locus_tag="IT904_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAP3193051.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            10684..12135
                     /gene="trkH"
                     /locus_tag="IT904_003313"
     CDS             10684..12135
                     /gene="trkH"
                     /locus_tag="IT904_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAP3193052.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            12147..12692
                     /gene="hemG"
                     /locus_tag="IT904_003314"
     CDS             12147..12692
                     /gene="hemG"
                     /locus_tag="IT904_003314"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAP3193053.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVTNVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         2926 a         3388 c         3568 g         3106 t
ORIGIN      
        1 actgacccag gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc
       61 gagcctcact aacctgacgc ccgaactgaa agcgtcgatg gatgaactac gccaggccgc
      121 ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca
      181 caccatccat aacccggtgg tgaaagataa tgaagctgcg catgagggcg taacgcctgc
      241 cgctgcacaa acgcaggcca gttcgccgga acagaagcca gaaaccacgc cagagccggt
      301 ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga
      361 taaaccgtaa acatgtctgt agaagatact caaccgctta tcacgcatct gattgagctg
      421 cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga tattcctgtg tctggtctat
      481 ttcgccaatg acatctatca cctggtatcc gcgccattga tcaagcagtt gccgcaaggt
      541 tcaacgatga tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt
      601 atggtgtcgc tgattctgtc agcgccggtg attctctatc aggtgtgggc atttatcgcc
      661 ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc tgctggtttc cagctctctg
      721 ctgttttata tcggcatggc attcgcctac tttgtggtct ttccgctggc atttggcttc
      781 cttgccaata ccgcgccgga aggggtgcag gtatccaccg acatcgccag ctatttaagc
      841 ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg aagtgccggt agcaattgtg
      901 ctgctgtgct ggatggggat tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg
      961 ctggttggtg cattcgttgt cgggatgttg ctgacgccgc cggatgtctt ctcgcaaacg
     1021 ctgttggcga tcccgatgta ctgtctgttt gaaatcggtg tcttcttctc acgcttttac
     1081 gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact
     1141 gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat ggagtacagg atgtttgata
     1201 tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg tgatgatgtt gtagcgtgcg
     1261 cttttgacgc gggagttaat gggctactca tcaccggcac taacctgcgt gaaagccagc
     1321 aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc aacggcgggc gtacatcctc
     1381 acgacagcag ccagtggcaa gctgcgactg aagaagcgat tattgagctg gccgcgcagc
     1441 cagaagtggt ggcgattggt gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg
     1501 aagagcagga acgcgctttt gttgcccagc tacgcattgc cgcagattta aacatgccgg
     1561 tatttatgca ctgtcgcgat gcccacgagc ggtttatgac attgctggag ccgtggctgg
     1621 ataaactgcc tggtgcggtt cttcattgct ttaccggcac acgcgaagag atgcaggcgt
     1681 gcgtggcgca tggaatttat atcggcatta ccggttgggt ttgcgatgaa cgacgcggac
     1741 tggagctgcg ggaacttttg ccgttgattc cggcggaaaa attactgatc gaaactgatg
     1801 cgccgtatct gctccctcgc gatctcacgc caaagccatc atcccggcgc aacgagccag
     1861 cccatctgcc ccatattttg caacgtattg cgcactggcg tggagaagat gccgcatggc
     1921 tggctgccac cacggatgct aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc
     1981 ggaactcggt attcttcaca ctgtgcttaa tctctttatt aataagatta agcaatagca
     2041 tggagcgagc ctcaccatcg ggttcggtga aaatggcctg aaagccttcg aacgcgcctt
     2101 cggtaataat caccttatca cccggataag gggttgccgg atcgacaatg tctttcggtt
     2161 tatataccga tagctgatga ataaccgccg atgggactat cgctggcgac gcgccaaagc
     2221 gcacgaagtg gctgacaccg cgggtcgcgt tgatagtcgt ggtatgaatc acttctggat
     2281 caaattccac aaacaggtag ttggggaaca atggctcact gactgcagta cgttttccac
     2341 gcacgatttt ttccagggtg atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt
     2401 gttcctgggc acgttgaagt tgcccgcgct tgcagtacag taaataccag gattgcataa
     2461 tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa gctttacgct aagttaatta
     2521 tattccccgg tttgcgttat accgtcagag ttcacgctaa tttaacaaat ttacagcatc
     2581 gcaaagatga acgccgtata atgggcgtag attaagaggc tacaatggac gccatgaaat
     2641 ataacgattt acgcgacttc ctgacgctgc ttgaacagca gggtgagcta aaacgtatca
     2701 cgctcccggt ggatccgcat ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg
     2761 gtgggcctgc gctgttgttc gaaaacccta aaggctactc aatgccggtg ctgtgcaacc
     2821 tgttcggtac gccaaagcgc gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc
     2881 gtgaagttgg taaattattg gcgtttctga aagagccgga gccgccaaaa ggtttccgcg
     2941 acctgtttga taaactgccg cagtttaagc aagtattgaa catgccgaca aagcggctgc
     3001 gtggtgcgcc ctgccaacaa aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc
     3061 ccattatgac ctgctggccg gaagatgccg caccgctgat tacctggggg ctgacagtga
     3121 cgcgcggccc gcataaagag cggcagaatc tgggcattta tcgccagcag ctgattggta
     3181 aaaacaaact gattatgcgc tggctgtcgc atcgcggcgg cgcgctggat tatcaggagt
     3241 ggtgtgcggc gcatccgggc gaacgtttcc cggtttctgt ggcgctgggt gccgatcctg
     3301 ccacgattct cggtgcagtc acccccgttc cggatacgct ttcagagtat gcgtttgccg
     3361 gattgctacg cggcaccaaa accgaagtag taaagtgtat ttccaatgac ctcgaagtgc
     3421 cagccagtgc cgaaatcgtc ctggaagggt atatcgatcc tggtgagatg gcgccggaag
     3481 ggccgtatgg tgaccacaca ggttactata atgaagtcga tagtttcccg gtgtttaccg
     3541 tgacgcatat tacccagcgt gaagatgcga tttaccattc cacctatacc gggcgtccgc
     3601 cagatgagcc cgcggtactg ggcgtggcgt tgaacgaagt gtttgtaccg attctgcaaa
     3661 aacagttccc ggaaattgtc gatttttacc tgccgccgga aggctgttct tatcgtctgg
     3721 cggtagtgac gatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct
     3781 ggtcgttctt acgccagttt atgtacacta aatttgtgat cgtttgcgat gatgacgtca
     3841 acgcacgcga ctggaacgat gtgatttggg cgattaccac ccgtatggac ccggcgcggg
     3901 atactgttct ggtagaaaat acgcctattg attatctgga ttttgcctcg cctgtctccg
     3961 ggctgggttc aaaaatgggg ctggatgcca cgaataaatg gccgggggaa acccagcgtg
     4021 aatggggacg tcccatcaaa aaagatccag atgttgtcgc gcatattgac gccatctggg
     4081 atgaactggc tatttttaac aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt
     4141 tatcgatccg acagagaaag cgcatgacaa ccttaagctg taaagtgacc tcggtagaag
     4201 ctatcacgga taccgtatat cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg
     4261 ctggtcagta tttgatggta gtgatggatg agcgcgacaa acgtccgttc tcaatggcct
     4321 cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg cgcttctgaa atcaaccttt
     4381 acgcgaaagc agtcatggac cgcatcctca aagatcatca aatcgtggtc gatattcccc
     4441 acggagaagc gtggctgcgc gatgatgaag agcgtccgat gattttgatt gcgggcggca
     4501 ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg
     4561 atatcaccat ttactggggc gggcgtgaag agcagcatct gtatgatctc tgcgagcttg
     4621 aggcgctttc gttaaagcac cctggtctgc aagtggtgcc ggtggttgaa caaccggaag
     4681 caggctggcg tgggcgtact ggtaccgtgt taacggcggt attgcaggat catggtacgc
     4741 tggcagagca tgatatctat attgccggac gttttgagat ggcgaaaatt gcccgtgacc
     4801 tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta
     4861 tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga acggcaacta aaactttatt
     4921 cagtggcatt tagatctatg acgtatctgg caaaccatgc ccgatgcgac gctgtcgcgt
     4981 cttatcgtgc ctacaaatag tccgaaccgt aggccggata aggcgtttac gccgcatccg
     5041 gcaattggtg cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc
     5101 cggatctgta gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca
     5161 aacaccgtcg caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg
     5221 tctttgcgct ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga acaacccagc
     5281 ggatgaccca gcgcgatcgc gccaccattg aggttgatct tctcgtcaat ctgctccatt
     5341 agcccgagat ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac
     5401 acgccgatat cgctggcaga aagccccgct tttttcagcg ccagctttga ggccggaacc
     5461 gggccgtaac ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg
     5521 cgcggcttaa gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg
     5581 ccatcggaaa gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc
     5641 ggacgcagcg tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta
     5701 aactgcttca ggacgccgtc ggcatcgtga ccaccggtcg gaatgatttc atttttaaat
     5761 gcggccgact gcgtggcggc ccaggcacga gcgtgcgatc gcgcggcaaa ggcatcctgc
     5821 atttcacggc tgataccgtg catacgcgcc agcatttctg ctgttaagcc catcatgccc
     5881 gccgctttgg cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc
     5941 acatggccca tatgctccac gccgccaacc agacatgcct gcgcatcgcc ggtcataatc
     6001 atccgtgctg cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc
     6061 gccgggacag agtgtggtac ttctgccagc agcgccgcgt tacgggcgat gttaaaaccc
     6121 tgctccagcg tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc
     6181 gccgggttac gcgccagcag gctacgcatt aaatgagcgg agagatcttc tgcacgcacg
     6241 ttacgaaaag caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca
     6301 acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca
     6361 accggaggat agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttca
     6421 tacagcgggc cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg
     6481 gtgtccagcc agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc
     6541 gccatatccg cttccgccgg agtggcgata atgccttcct ccagacagcg caccacttcg
     6601 ttgaccatcg ggatcatcat gcgggcgata atctcctctt cgctgaaatc gcgcttcggc
     6661 tggctcactt ctgccagcag gtcttcaacg gcggcgtctt cttctttctt cggcttacct
     6721 ttgctgtctt ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca
     6781 tcaaacagcg cgtcgatggc atcgcggtaa tctttctgca tccgctgcgg gaagcctgct
     6841 gccatgacag cctgagcgtg atgcgcggta tcaatcccca caacgtccag caggtatgcc
     6901 ggacccatcg gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaagtctgcg
     6961 ccgtcgcgca gcaactggct gaatcctgcg aagtagggga acagtacacg gttaacgaag
     7021 aagccggggc agtcgttgac cacaatcggc gtcttgccca tcttgctcgc ccatgcgacg
     7081 actttcgcga tggtttcgtc ggagcttttc tcgccgcgaa taatttctac caacggcatc
     7141 cggtgcaccg ggttaaagaa gtgcatcccg cagaagtttt ccggacgttc cagcgcgttg
     7201 gccagttcgc tgataggaat ggttgaagtg ttagacgcca gcacggtatc ctggcgtact
     7261 ttttgttcgg tttctgccag tacggctttt ttcactttcg ggttttcaac aaccgcttct
     7321 accacaacat ccacgcggtc aaacccggcg tagtcgagcg ttgggtggat tgtggagatc
     7381 acgccagcca gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc
     7441 gcggcttcgg tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc
     7501 acgcctttcc acgcagactg gtaagcgatg ccgccgccca taatgcctgc gcccagcaca
     7561 gcggcctgtt tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat
     7621 tgatcgttaa ggaatatgcc gaccagtgcg cgagcttcgt tggtatgcgc cagcgggaca
     7681 aaacttttgt tttccaggtt tagggcttct tcacgaccaa aacgggccgc agcttcaatg
     7741 gtttttactg cggtgatggg ggccggataa tgtttccccg ccgtttgtgc gaccatccct
     7801 ttagcgatgg tgaagctcat ggtggcttca atcttgctca gttttagcgg ttccagcttc
     7861 ggctgacgtt tcgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aatcgccatc
     7921 gccccttcaa tcagtttttc tgctttgact acgccatcca ccagaccgat tttcagcgcc
     7981 tgatccgcgc cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc
     8041 atacgtggca tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc
     8101 agaccgatgc gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag
     8161 ccaccgccca gcgcatagcc attgacggca gcaatggtcg gcaccggcag atcttccagg
     8221 cgattaaaca cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg
     8281 aacagggaca aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgt
     8341 agcagcagcc cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg
     8401 gcgacggtcg cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca
     8461 atgccatctt ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc
     8521 tgaatccgca aggtgatctg gtcgtaccag atgagtcgaa gtgtgtattt tgtgttaaaa
     8581 atatgcaaac aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc
     8641 tggatgaacg atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa
     8701 tggaatcact ggcctcgctc tataaaaatc atatagctac cttacaggaa cggactcgcg
     8761 atgcgctggc gcgcttcaag ctggatgcgt tacttattca ctccggcgag ctgttcaatg
     8821 tttttctcga cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc
     8881 cggtaactca ggtgccaaac tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt
     8941 ggttctatct gccggttgat tactggcaca acgtcgaacc gctgccgacc tccttctgga
     9001 ctgaagatgt ggaagtgatc gcactgccga aagccgatgg cattggtagc ctgctgcccg
     9061 ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga gcgtgcgctg caactgggta
     9121 ttgaggccag caacatcaac ccgaaagggg ttatcgacta cctgcattac tatcgctcct
     9181 tcaaaaccga gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc
     9241 atcgtgcggc agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacattgcct
     9301 atctgaccgc gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta
     9361 acgaacacgc tgcggtgctg cattacacca aactggatca tcaggcgccg gaagagatgc
     9421 gcagcttcct gctggatgcc ggggccgaat ataacggcta tgcggctgac ctgacccgta
     9481 cctggtcggc aaaaagtgac aacgattacg cacagctggt gaaagacgta aatgatgaac
     9541 aactggcgct gatcgcgacc atgaaagctg gcgttagcta tgtggattac cacatccagt
     9601 tccatcagcg catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag
     9661 aagcgatggt cgaaaacgat ctcaccggac cgtttatgcc gcacggtatc ggccatccgc
     9721 tgggcctgca ggtgcatgac gtcgccggtt ttatgcagga tgatagcggt acgcacctcg
     9781 cggcaccggc aaaatatccg tacctgcgct gcacccgtat tctccagccg ggcatggtgt
     9841 taaccatcga accgggtatc tacttcattg aatcgctact ggcaccgtgg cgtgaagggc
     9901 agttcagcaa gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc
     9961 gtatcgaaga caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga
    10021 aactggcgtg atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat
    10081 caaaaagagc cgcttcataa cactgttggc gcataccgat ggcgttgagg cggcgaaagc
    10141 gtttgttgaa tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt
    10201 ggcgggtgcg ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcagg
    10261 aacggcaggt aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc
    10321 tgtggtagtg cgctactacg gtggcatatt gcttggcacc ggtgggttag tgaaagcgta
    10381 tggcggcggc gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac
    10441 cgaatatact ttgcaatgtg aatatagtca gttaaccggc attgaagcgt tgctggggca
    10501 gtgtgacggc aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct
    10561 tccggcggcg aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt
    10621 gcaattgtta gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca
    10681 gagatgcatt ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg
    10741 accatgatta tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt
    10801 acccagacct tttttgtcgc cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa
    10861 gagaagggcg aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ttggaccgtg
    10921 ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc
    10981 gatgcgtttt ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg
    11041 ctggattcgc tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg
    11101 atggggatca tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag
    11161 ctctatcgcg cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg
    11221 gaaacggcga aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg
    11281 ttgtggtttg ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct
    11341 attggcggct tctcgacaca tgatgccagt atcggttatt tcgacagccc gactattaac
    11401 actatcattg ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg
    11461 ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc
    11521 gtacagttta cgctggtggt tatttgtaca ctcgtactgt ggtttcataa tgtctacagt
    11581 tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc
    11641 gggtttacga ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt
    11701 tcagctttta tcggcggttg tgccgggtca acgggcggtg gcctgaaagt gatccgcatc
    11761 ctgctgctgt ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc
    11821 tatagcatta agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga
    11881 tttttctccg cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc
    11941 gtggatgact tttctgcttt tgcctcggtt gttgcgacat tgaataacct ggggccgggg
    12001 cttggcgtgg ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt
    12061 gccaacatgc tgtttggtcg tctcgaggtc tttacattgc tggtgctctt taccccgact
    12121 ttctggcgtg aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg
    12181 gacaaacgcg cgagattgcc tcctacttgg cttcggaact gaaagaactg gggatccagg
    12241 cggatgtcac caatgtgcac cgcattgaag aaccacagtg ggaaaactac gaccgtgtgg
    12301 tcattggtgc ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa
    12361 agcatgcgac gcggctgaat tcgatgccga gcgcctttta ctccgtaaac ctggtggcgc
    12421 gtaaaccgga gaagcgcact ccacagacca acagctacgc gcggaagttt ctgatgaact
    12481 cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc
    12541 gctggtacga ccgttttatg atcaagctga ttatgaagat gtcaggcggt gaaacggata
    12601 cgcgcaaaga agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg
    12661 cccatttaac cgacaaaccg acgctgaaat aagcacaaag aataaaaaat gcgcggtcag
    12721 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact
    12781 gacacggaac aacggcaaac acgtcgccgg gtcagcgggg ttctcctgag aactccggca
    12841 gagaaagcaa aaataaatgc ttgactctgt agcgggaaag cgtattatgc acaccccgcg
    12901 ccgctgagaa aaagcgaagc ggcactgctc tttaacaatt tatcagacaa tctgtgtggg
    12961 cactcgaaga tacggattct taacgtcg
//
LOCUS       DACAPB010000132        12912 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.132, whole genome shotgun sequence.
ACCESSION   DACAPB010000132 DACAPB010000000
VERSION     DACAPB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.132"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(22..1236)
                     /gene="ydcO"
                     /locus_tag="IT904_003315"
     CDS             complement(22..1236)
                     /gene="ydcO"
                     /locus_tag="IT904_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HAP3193054.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLITASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1289..1825
                     /locus_tag="IT904_003316"
     CDS             1289..1825
                     /locus_tag="IT904_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3193055.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1898..3859
                     /gene="rlhA"
                     /locus_tag="IT904_003317"
     CDS             1898..3859
                     /gene="rlhA"
                     /locus_tag="IT904_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HAP3193056.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3951..4181)
                     /locus_tag="IT904_003318"
     CDS             complement(3951..4181)
                     /locus_tag="IT904_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HAP3193057.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4403..4579
                     /gene="hicA"
                     /locus_tag="IT904_003319"
     CDS             4403..4579
                     /gene="hicA"
                     /locus_tag="IT904_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HAP3193058.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4625..5041
                     /gene="hicB"
                     /locus_tag="IT904_003320"
     CDS             4625..5041
                     /gene="hicB"
                     /locus_tag="IT904_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HAP3193059.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5120..6526
                     /locus_tag="IT904_003321"
     CDS             5120..6526
                     /locus_tag="IT904_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP3193060.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6771..7916
                     /locus_tag="IT904_003322"
     CDS             6771..7916
                     /locus_tag="IT904_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP3193061.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISEPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDNTVQMSDFKNESVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7934..8947
                     /locus_tag="IT904_003323"
     CDS             7934..8947
                     /locus_tag="IT904_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP3193062.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAENLCGMTGSFALRPEHIRLNIPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8948..9889
                     /locus_tag="IT904_003324"
     CDS             8948..9889
                     /locus_tag="IT904_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3193063.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            9879..10673
                     /locus_tag="IT904_003325"
     CDS             9879..10673
                     /locus_tag="IT904_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3193064.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10695..12119
                     /gene="patD"
                     /locus_tag="IT904_003326"
     CDS             10695..12119
                     /gene="patD"
                     /locus_tag="IT904_003326"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAP3193065.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12216..12311)
                     /gene="yncL"
                     /locus_tag="IT904_003327"
     CDS             complement(12216..12311)
                     /gene="yncL"
                     /locus_tag="IT904_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAP3193066.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12506..12679
                     /locus_tag="IT904_003328"
     CDS             12506..12679
                     /locus_tag="IT904_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAP3193067.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12765..>12912
                     /gene="ydcY"
                     /locus_tag="IT904_003329"
     CDS             12765..>12912
                     /gene="ydcY"
                     /locus_tag="IT904_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HAP3193068.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLR"
BASE COUNT         3142 a         3158 c         3424 g         3188 t
ORIGIN      
        1 tccttcaggg cgtggaggat gtcaatatcg gtttctgtca gcgattaaat tcaacaccac
       61 gtaacaaacg cctccggcaa ttaatcccca aaacgcagaa ccaatcccga ccagcgtcaa
      121 tccacttgcc gttatcagaa atgccaccac cgccgcgtct cgctcacgct cattatgcag
      181 cgcctgatac aaactgccgc cgatggtact taacagcgcc agacctgcca gcatctggat
      241 ccaacttacg ggcagggcag ccatcatccc ggtaatggca ctaccaaaca gacctgcgag
      301 caaatagaaa atgcctgcaa cggcagcggc cagccaacgt tgatctttat ccggatgcgc
      361 ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa
      421 aggggaaaaa accagtgcca gcaatccagt aaatacaatt aatggcgaaa caggagccga
      481 atatccagct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
      541 aaaaaggggg agtgcaacgc tcaggctgtg agcaaacgaa aaatcagggg taatataagt
      601 ggggagaacg ggtttaaaga caacatcagt tgtgacaacg tcaccttgcg cgatgacgat
      661 cacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccttggttgc
      721 cagccatacc agcaacatac ttccacacaa cgtaaattga ccgtccagac tggcaaacgc
      781 ctgtaaacca aagcgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
      841 aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta gcgcgttggt
      901 gacaataaaa acgccgatgg cttcgttaag tgttagtccc tgcaatccgg tgaccaacaa
      961 agccgcgcca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagt
     1021 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
     1081 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
     1141 taataccgcc agaaaccccg ccagtagcgt gggtggagga atagaaaaca gacgcatagt
     1201 cacctcgtgc gttatagcgt acaggcaccg tagcatttgt ccgttataac gcacaagtga
     1261 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgctttt tatccaccac
     1321 acttaaacaa ctacgccagc agcgcggctg gagtctttcg cgactggcag aagcgacggg
     1381 cgtttctaaa gcaatgttag ggcagattga gcgtaatgag tccagtccga cggtcgcgac
     1441 attatggaaa attgccaccg ggttgaacgt accattttca acatttattt ctccgccgca
     1501 gtccgctacg ccttcagttt atgatccaca acagcaggcg atggtgatca cttcgctgtt
     1561 tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcat ctggtgcaat
     1621 aagcgaatcg acgccccatg agaagggggt aatcgaacat gtcgtcgtca ttgatggaca
     1681 acttgatctg tgcgttgatg gggaatggca gacccttaat tgtggggaag gcgttcgatt
     1741 tgctgcagac gtcacgcata tctatcgtaa tggtggggag caaaccgtac attttcattc
     1801 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
     1861 taaaatagcc gccatttttc agctactgga taagaatgtg accgtatctt ctcatcgact
     1921 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
     1981 cgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagctt
     2041 gaaagatatt gccgagctgg tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac
     2101 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttactgacct
     2161 ctaccagacc ggtgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
     2221 tccgccgatt gaactgcacg ccagtacgca gtgcgacatt cgtacagttg aaaaagcgaa
     2281 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagagctga atcttgatca
     2341 gatccgcgcg attcaccagg ctacggacgc gaccattgaa ttctttattc atggggcact
     2401 gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc gtagcgccaa
     2461 ccgtggcgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
     2521 ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga ctgccaacct
     2581 cggcgcgctg attgatgctg gtgtacgctc cttcaagatt gaagggcgtt acaaagatat
     2641 gagctacgtg aagaatatca ccgcccatta tcgccagatg cttgatgcca ttattgaaga
     2701 acgtggcgat ctggcgcgcg cttcatcagg tcgtactgaa catttctttg ttccatcgac
     2761 ggaaaagact ttccaccgtg gtagcacaga ttattttgtg aatgcccgta aaggcgatat
     2821 tggcgcgttc gattcgccga aatttatcgg cctgccggta ggcgaagtat tgaaagtggc
     2881 gaaagatcat ctcgatgttg ccgttaccga gccactggca aatggcgatg gcctgaacgt
     2941 gttgattaaa cgtgaagtcg tcggttttcg tgccaatacg gtcgagaaaa ccggagaaaa
     3001 tcagtaccgc gtctggccca atgaaatgcc agcagatttg cacaaaattc gtccacatca
     3061 cccactaaac cgtaatcttg atcataactg gcagcaggca ctgacaaaaa cctccagcga
     3121 acgtcgggtg gcggtagaca ttgaactggg cggctggcag gaacaactga ttctgaccct
     3181 caccagtgaa gagggtgtca gcatcacgca tacgctggac gggcagttcg acgaagccaa
     3241 taacgccgaa aaagcaatga acaatctgaa ggatggtctg gcaaaactgg ggcaaaccct
     3301 ctattacgcc cgcgatgtgc aaattaattt gccgggggcg ctgtttgtac caaacagtct
     3361 gttaaaccag ttccgccgtg aagctgctga catgctggat gctgcgcgtc ttgccagtta
     3421 ccagcgcggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc aaacgcatct
     3481 gagtttcctc gcgaacgtat acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt
     3541 gcagctgatt gacgcggcgt atgaagcaca tgaagagaag ggcgaagtcc cggtgatgat
     3601 caccaagcat tgtctgcgct ttgcctttaa tctgtgcccg aaacaggcga aaggcaatat
     3661 caaaagctgg aaggcgacgc caatgcaact ggttaacggc gatgaagtat taacgctaaa
     3721 gtttgattgc cgcccatgcg agatgcacgt cattggcaaa atcaaaaatc acatactgaa
     3781 aatgccgtta ccgggaagcg tagtggcatc cgtaagtccg gatgagctgc tgaaaacatt
     3841 gcctaagcga aaagggtaaa acgccagttt tctggttact cacaactcat tgaatctgca
     3901 tgatattgcc tgccgggtaa ggcgtcacgc cgcatccggc aacaaatgac tcaacgttgt
     3961 cccgcttctg gtttgcgcga tttttgccag taaaaatgtt cgcgcaaacc ttccgccgac
     4021 tcttccgcca cagcacgcaa ttcatcgctg tccgcttcat gacgcagctg atgatccaca
     4081 ttctttaccc acacaaattc atgtcctttg tgccctgcca tgagttgtcc tgagaacagc
     4141 gcacacgtta ataagacaac cgataacgcc ttcgtaaaca tcctgccacc tttttcttac
     4201 cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg caaattagcg
     4261 caaagaaatt ctggaatctt ccttcctgat tttgcatggc attctgccgt tgcggcgatt
     4321 tagtgctatt ttcgagcaaa ttacacacgg aggtaaacac taatgtttac tttgttgata
     4381 tactcagcgg cagggaggcg atgtgaaaca aagcgagttc agacgttggc tcgaatctca
     4441 gggcgtcgat gtagcgaatg gcagcaacca tttgaaactc aggtttcatg ggaggcgcag
     4501 tgtcatgccg cgtcacccct gcgatgagat taaagaacca ttgcgtaaag caatcctgaa
     4561 acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag ggacagtcga
     4621 aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata tggtttcttt
     4681 tgtggatatc cctgaagcgt tgacgcaggg cgaaactgtc gctgaagcga tggaagcggc
     4741 aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc ttatcccttt
     4801 accttcgcca ttaaatagtc atgatcactt tattgaagtg cctttgagcg tcgcctctaa
     4861 ggtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt tagccaggcg
     4921 aattggcaaa cctaaacagg agattactcg cctatttaac ttgcatcatg cgacaaaaat
     4981 cgacgccgtc cagctcgcgg caaaggcgct tggcaaagag ttatcgctgg tgatggttta
     5041 attacagtta acgaaaagtt gtcattttta acaactgata tagactgccg aatcatctgc
     5101 acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat tacgcgagca
     5161 gattgcgtcg ggtatctggc aacccggcga tcgtttgcct tcgttgcgtg accaggtggc
     5221 gctttcaggc atgagcttta tgactgtcag ccatgcctat cagttgctcg aaagtcaggg
     5281 atatattatc gcacgaccgc agtcgggtta ttacgttgcg ccacaggcaa taaaaatgcc
     5341 gaaagcgcca gtcattccag tcactcgaga tgaagcagtc gatatcaaca cttatatttt
     5401 tgatatgttg caggccagtc gcgatccgtc ggtcgttccg tttgcctcgg cctttcccga
     5461 cccgcgactt ttccccctcc aacaactaaa ccgctcgctg gcgcaggtaa gcaaaaccgc
     5521 cacagcgatg agcgtgattg aaaacttacc gccaggaaac gcagaactgc gtcaggctat
     5581 tgctcgtcgc tatgccttac agggcatcac catttctcct gatgaaattg tcattactgc
     5641 cggggcgtta gaggcattaa acctcagttt gcaagcggta actgaaccgg gcgattgggt
     5701 gatagtagag aatccttgtt tctacggtgc gttgcaggcg ctggagcggc tacggctgaa
     5761 ggcgttatcg gtggcgacgg atgttaaaga agggatagat cttcaggcgc tggaactggc
     5821 gttgcaggag tatccggtga aagcgtgctg gctgatgact aatagccaga atccactcgg
     5881 atttacctta acgccgcaaa aaaaagcaca actggtggcg ttgctcaatc agtacaacgt
     5941 aacgctgatt gaagatgacg tttacagcga actttatttt ggacgggaaa aaccgctgcc
     6001 tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt cgaaatgtct
     6061 ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcacgta aaattcaacg
     6121 cttgcagttg atgagtacgc tttccaccag ctcaccgatg caacttgcgc tggtggatta
     6181 cctttccacg cgccgatacg acgcccatct tcgtcgcctg cgtcgccagc ttgcggaacg
     6241 taaacaacgt gcctggcagg cactgctgcg ttatctgcct gcggaagtga aaattcatca
     6301 taatgacagt ggttactttc tctggctgga gctccccgag ccgttagatg ccggcgaatt
     6361 aagcctggcg gcactgacgc atcatatcag tattgcgccg ggtaaaatgt tttctaccgg
     6421 tgaaaactgg tcacgttttt tccgttttaa taccgcgtgg cagtggggag agcgtgaaga
     6481 acaggcggta aaacaattag gcaaacttat tcaagaacgg ctgtaatagc gtttaattta
     6541 attcctctta gattgggtaa tatgaatttc gaatagcagt catatttcct aactccttga
     6601 ctatactcca gaagataacc ttacagacgg cataatgcgc ggtagctcac aacctgatta
     6661 aattttctca ggggcgaagg tgtgcctgca agccgccgtc tatggttaaa caaggagata
     6721 tttttacggc acggcggctg aacaattaat tacgacagga gtaagacctt atgagcaaga
     6781 catttgcccg cagcagcctg tgtgcgctca gcatgacaat aatgaccgct cacgccgccg
     6841 aaccgcctac caatttagat aaaccggaag ggcgactgga tattatcgcc tggccgggat
     6901 acatcgaacg cggacaaact gataaacaat acgactgggt aacgcagttc gaaaaagaga
     6961 caggctgcgc ggtgaatgtg aaaaccgccg cgacttccga tgaaatggtc agtctgatga
     7021 ccaaaggggg ttacgatctg gttacggcat ccggcgatgc ctcgctgcgt ttgattatgg
     7081 gtaaacgcgt gcagccgatt aataccgcat tgattcccaa ctggaaaacg ctcgatccgc
     7141 gcgtggttaa aggcgactgg tttaatgttg gcggcaaagt ttacggcaca ccttaccaat
     7201 gggggccgaa cctgctgatg tacaacacta aaaccttccc gacgccgccg gatagctggc
     7261 aagtggtttt tgttgagcaa aatctgccgg acggcaagag caataaaggc cgcgttcagg
     7321 cttatgatgg ccctatctac attgcggacg ctgcgttgtt cgttaaagcc actcagccgc
     7381 agttgggcat cagcgaaccg tatcaactca ccgaagaaca gtaccaggcg gtgctgaaag
     7441 tgctgcgcgc tcaacacagt ttgatccatc gctactggca tgacaatacc gtgcaaatga
     7501 gcgatttcaa aaacgagagt gtggttgctt ccagtgcctg gccctatcag gccaacgccc
     7561 tgaaagccga aggccagcct gttgctaccg ttttcccgaa ggagggtgtt accggttggg
     7621 ctgataccac catgctgcat agcgaagcga aacatccggt ttgcgcctac aaatggatga
     7681 actggtcatt aacgccaaaa gtgcagggcg atgtggcagc ctggtttggc tcgttaccgg
     7741 tagtgccgga agggtgtaaa gccagtccgt tattaggcga aaaaggttgt gaaaccaacg
     7801 gttttaacta tttcgacaaa atcgccttct ggaaaacgcc tatagcagaa gggggcaagt
     7861 ttgttcccta cagtcgctgg acgcaggatt acattgccat tatgggcggt cgctaacttc
     7921 gctggggtgc tttatgacgt acgcagtgga gtttgacaac gtctcgcggt tgtacggtga
     7981 cgtgcgcgca gtagatggcg tcagtattgc gataaaagat ggtgagttct tctctatgct
     8041 ggggccgtcc ggctccggca aaaccacctg cctgcgcctg attgctggct tcgaacagct
     8101 ttccggcggg gctatctcta tctttggtaa acccgccagc aatctgccac cgtgggagcg
     8161 ggacgtgaat actgtctttc aggactacgc gctatttccg catatgtcga ttcttgacaa
     8221 tgtcgcctat gggctgatgg tcaaaggcgt gaataaaaag cagcggcacg caatggcgca
     8281 agaggcgctg gagaaagtgg cgttggggtt tgtacatcaa cgtaaaccgt cacaactttc
     8341 tggtggtcag cgccagcggg ttgctatcgc cagagcattg gtgaatgaac cgcgcgtatt
     8401 gctgttggat gaaccgctcg gcgcactgga tctcaaattg cgtgagcaga tgcagctgga
     8461 actgaaaaaa ctgcaacagt ctctcggtat cacttttatc ttcgttaccc acgatcaggg
     8521 cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat aatggacgca ttgagcaggt
     8581 cgattccccg cgcgatctct atatgcgccc gcgcacgccg tttgttgccg gattcgtcgg
     8641 gacatccaac gtttttgatc cgctaatggc cgagaatctt tgcggcatga cgggaagctt
     8701 cgccctgcga ccggaacata tccgtctcaa tatccccggc gaaatgcagg ctaatggcac
     8761 gatccaggcg gtgcaatatc agggcgcagc gacccgtttt gaactgacac tgagcggcgg
     8821 tgaaaaactg ctggtgagtc aggccaatat gacaggcgaa gaactgcctg ccacgctcac
     8881 gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg atggtgccgc tggttgagga
     8941 gaggtgaatg gcgatgaatg tattgcaatc accttcacgt ccaggtctgg gtaaggtgtc
     9001 cggtttcttc tggcgtaatc cggggctggg gctgttttta ctgctgcttg gcccgctaat
     9061 gtggtttggc attgtctatt tcggctcgct gctgacactg ttatggcagg gattttatac
     9121 ttttgacgat ttcaccatgt cggtaacgcc ggaactgacg ctggcgaata tccgtgcgct
     9181 gtttaatccg gcgaattacg acatcattct ccgcacgctg actatggctg tggcagtcac
     9241 tatcgccagc gccattctgg cttttccaat ggcgtggtat atggcgcgct ataccagcgg
     9301 gaaaatgaaa gcgttttttt atattgcggt aatgttgccg atgtgggcga gctacattgt
     9361 taaagcctat gcctggacgt tattgctggc aaaagatggc gtggctcagt ggtttttaca
     9421 acatcttggg ctggaaccac tgctgactgc gttccttaca ttacctgcgg tgggcggaaa
     9481 tacgctgtca acttccgggc tggggcgctt tctggtgttt ctctatatct ggttgccgtt
     9541 catgatcctg cccgttcagg cggcgcttga gcgtttgccg ccgtcattgt tgcaggcgtc
     9601 ggctgatctc ggcgcacgtc cacgacaaac ctttcgctat gtggtgctgc cgctggcaat
     9661 cccgggtatt gccgctggct ctatctttac cttctcactc acactgggcg attttatcgt
     9721 cccgcagctg gttggtcctc caggatattt tatcggcaat atggtttatt cccagcaggg
     9781 ggcgattggc aatatgccga tggcggcggc attcaccctg gtgccgatta ttctcatcgc
     9841 actgtacctg gcgttcgtga aacgtctggg agcgttcgat gcactctgaa cgcgcaccgt
     9901 ttttcctcaa actggcggcc tggggcggcg ttgttttcct acattttccc atcctgataa
     9961 tcgccgccta tgcgtttaac actgaagatg cggcgtttag ttttccaccg cagggcctga
    10021 cgctgcgctg gtttagcgtg gcagcacagc gtagtgatat tcttgatgcc gtgacactgt
    10081 cacttaaagt ggcggcgctg gcgacattaa ttgcgctggt gttagggacg ctggcagctg
    10141 ccgcgctgtg gcgacgagac tttttcggca aaaacgccat ttcgctgtta ctgctgctgc
    10201 ccattgcgct gccgggcatt gtcactggtc tggcgttatt aaccgccttt aaaaccatca
    10261 atctggagcc gggatttttc accatcgtgg tcggtcatgc gactttttgt gtagttgtgg
    10321 tgtttaacaa tgtcatcgcc cgttttcgcc gcacctcctg gagtctggtt gaggcgtcaa
    10381 tggatcttgg ggccaatggc tggcaaacct tccgctacgt agtgttgccg aatctcagtt
    10441 cggcgttact ggcaggagga atgctggcgt ttgccttgtc gttcgatgaa atcatcgtta
    10501 cgacctttac ggcaggtcat gaacgaacgt taccgttgtg gttgctcaat cagcttgggc
    10561 gaccgcgtga tgtaccggta actaacgtgg tggcactgct ggttatgttg gtaacaacct
    10621 tgccgatcct gggggcctgg tggctaaccc gcgaaggcga caatggtcaa taaccactga
    10681 tacaggaata tgctatgcaa cataagttac tgattaacgg agaactggtt agcggcgaag
    10741 gggaaaaaca gcctgtctat aatccggcaa cgggggacgt tttactggaa attgccgagg
    10801 catccgcaga gcaggtcgat gctgctgtgc gcgcggcaga tgcagcattt gccgaatggg
    10861 ggcaaaccac gccgaaagtg cgtgcggaat gtctgctgaa actggctgat gttatcgaag
    10921 aaaatggtca ggtttttgcc gaactggagt cccgtaattg tggcaaaccg ctgcatagtg
    10981 cgttcaatga tgaaatcccg gcgattgtcg atgtttttcg ctttttcgcg ggtgcggcgc
    11041 gctgtctgaa tggtctggcg gcaggtgaat atcttgaagg tcatacttcg atgatccgtc
    11101 gcgatccgtt gggggtcgtg gcttctatcg caccgtggaa ttatccgctg atgatggccg
    11161 cgtggaaact tgctccggcg ctggcggcag ggaactgcgt agtgcttaaa ccatcagaaa
    11221 ttaccccgct gaccgcgttg aagttggcag agctggcgaa agatatcttc ccggcaggcg
    11281 tgattaacat actgtttggc agaggcaaaa cggtgggtga tccgctgacc ggtcatccca
    11341 aagtgcggat ggtgtcgctg acgggctcta tcgccaccgg cgagcacatc atcagccata
    11401 ccgcgtcgtc cattaagcgt actcatatgg aacttggtgg caaagcgcca gtgattgttt
    11461 ttgatgatgc ggatattgaa gcagtggtcg aaggtgtacg tacatttggc tattacaatg
    11521 ctggacagga ttgtactgcg gcttgtcgga tctacgcgca aaaaggcatt tacgatacgc
    11581 tggtggaaaa actgggtgct gcggtggcaa cgttaaaatc tggtgcgccg gatgacgagt
    11641 ctacggagct tggaccttta agctcgctgg cgcatctcga acgcgtcagc aaggcagtag
    11701 aagaggcgaa agcgacaggg cacatcaaag tgatcactgg cggtgaaaag cgcaagggta
    11761 atggctatta ctatgcgccg acgctgctgg ctggcgcatt acaggacgat gccatcgtgc
    11821 aaaaagaggt atttggtcca gtagtgagtg ttacgctctt cgacaacgaa gaacaggtgg
    11881 tgaactgggc gaatgacagc cagtacggac ttgcatcttc ggtatggacg aaagatgtgg
    11941 gcagggcgca tcgcgtcagc gcacggctgc aatatggttg tacttgggtc aatacccatt
    12001 tcatgctggt aagtgaaatg ccgcacggtg gacagaaact ttctggttac ggcaaggata
    12061 tgtcacttta tgggctggag gattacaccg tcgtccgcca cgtcatggtt aaacattaag
    12121 gataatattg cagatcgtaa gagtatgaga tgatcttgcg tactgtcaga gcactgattt
    12181 ctctgacagt acgtgctgcg tgcaaaatca ctacatcaaa taaaccagcc aaatctcata
    12241 gagataagag taaacaaacc aacagcagca aagacattta tcagtactac agttctactg
    12301 gaaacattca tttttgccac cttttatcat tgcccctatg tgtatagtac gggttaagaa
    12361 aatccgcaat aatactcatt ctgaagatga ataagggcaa gttgctgttt gatgattttt
    12421 tctcaaactg gtgaaaaaac ttgatgcacg tcaaaaaatg acgcatattt gcgcgcgttt
    12481 tattcatctg gctggacgcc cgtacatgtc tctctatcaa cacatgcttg ttttttatgc
    12541 ggttatggca gcaatcgcat ttcttatcac ctggtttctt tctcacgata agaaacgcat
    12601 ccgtttctta agcgcctttc tggtgggggc aacatggccg atgagtttcc cggtggcgct
    12661 gttgttttca ctgttttaaa tcttactcac tggcttgacg gcaccacgca gacttatatc
    12721 atttggatga atcgataaat ttcacaagtg gctaaggaga aagtatgtcg catctggatg
    12781 aagtcatcgc gcgcgtggat gccgcgattg aagagagcgt cattgcccat atgaacgaat
    12841 tattaatcgc cctgagcgat gacgcggagt taagtcggga agatcgctac acccagcagc
    12901 aacgtctgcg ca
//
LOCUS       DACAPB010000133        12870 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.133, whole genome shotgun sequence.
ACCESSION   DACAPB010000133 DACAPB010000000
VERSION     DACAPB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.133"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(56..1606)
                     /gene="cueO"
                     /locus_tag="IT904_003330"
     CDS             complement(56..1606)
                     /gene="cueO"
                     /locus_tag="IT904_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAP3193069.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            1772..2119
                     /locus_tag="IT904_003331"
     CDS             1772..2119
                     /locus_tag="IT904_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAP3193070.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNVQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            2225..3091
                     /gene="speE"
                     /locus_tag="IT904_003332"
     CDS             2225..3091
                     /gene="speE"
                     /locus_tag="IT904_003332"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAP3193071.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNSAIHTAAFALP
                     QYLQDALASQPS"
     gene            3107..3901
                     /gene="speD"
                     /locus_tag="IT904_003333"
     CDS             3107..3901
                     /gene="speD"
                     /locus_tag="IT904_003333"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAP3193072.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(3939..4301)
                     /locus_tag="IT904_003334"
     CDS             complement(3939..4301)
                     /locus_tag="IT904_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAP3193073.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(4476..7073)
                     /gene="acnB"
                     /locus_tag="IT904_003335"
     CDS             complement(4476..7073)
                     /gene="acnB"
                     /locus_tag="IT904_003335"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAP3193074.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVTQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            7428..9281
                     /locus_tag="IT904_003336"
     CDS             7428..9281
                     /locus_tag="IT904_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAP3193075.1"
                     /translation="MKMTLPFKPHVLALICSAGLCATSTGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTATPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITQDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQKQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(9352..10776)
                     /gene="lpdA"
                     /locus_tag="IT904_003337"
     CDS             complement(9352..10776)
                     /gene="lpdA"
                     /locus_tag="IT904_003337"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP3193076.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(11101..>12870)
                     /gene="aceF"
                     /locus_tag="IT904_003338"
     CDS             complement(11101..>12870)
                     /gene="aceF"
                     /locus_tag="IT904_003338"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP3193077.1"
                     /translation="ASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAP
                     AQAEEKKEAAPAAAPAAAAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGD
                     KASMEVPAPFAGTVKEIKVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPA
                     PAAGVKEVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVK
                     ELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGK
                     SEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAA
                     PAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTD
                     ITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLK
                     KYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFT
                     ISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVID
                     GADGARFITIINNTLSDIRRLVM"
BASE COUNT         3013 a         3636 c         3152 g         3069 t
ORIGIN      
        1 ttgtcatcag tttaatgccc ggagagatcc gggcatattt ccgaatacgg tctttttata
       61 ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag tgcgccatat
      121 aagcatgttc tttcggtgca tcgtgattaa acttcaccag cacttcgctg acattacctt
      181 ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg ccattttctg
      241 acaagatacg gaactgcgtg ccgtggatat ggaacggatg cagcatcatg tcgcccacgc
      301 cagagataac ccaacgttcg tattgccctt tcgccgccgc aaacatcggc ttgttcatat
      361 caaacgcctg accgttgatt ttgttggcat ggtggaaatc gaacttcccg ccgtggttca
      421 tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc atcccggcca
      481 tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg agcatcgggt
      541 ccatagagag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc
      601 tacttaatgt gtctggcaaa gcaccggagg cactaatagc aatcggctga atccgcatta
      661 ccggatgagg cttatcaaac ggcgcaatcg ccatccccat ctggctgacc ggcagcgtca
      721 ccaggtcaaa gggtttgtta tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca
      781 gcaccggcag ttcgctcacc ttcactggtt caggtagcag accaccgtcg ctggcaatca
      841 catacagcgg gcgattgtcg ctggtggcga aattgagcga acgggcatta cagccattga
      901 gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg cgggtagatt gcaccgttgg
      961 tcagcaacgt atcgccaaac cagcccacgg cggcggtcat cacatccagt tgataatcaa
     1021 tctgcccgtc ggcgctaaat tttttatcct gaacgatcac cggaacatca tcgatacccc
     1081 actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc agcccagcca
     1141 gccccatcgc cacctgtcgc ccggttttgc cgtgctgatg cggatggaac cagcaggtag
     1201 cggcaggttg atcaacgttc aacgtcaccg agcgcttgcc acctggcgga ataattccct
     1261 gcgggccgcc gtcgacttca cccggtactt ccagcccgtg ccagtgcaac gttgtctctt
     1321 ccgtcagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg
     1381 gccccagcag attgccgtta tagccccagg tagttgcagt tttcccgcca aaggtggact
     1441 ggcctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga
     1501 tcggtaacgt tgggcgttct gccgcaaata ctgcgcggct ccacagcggc aaagccgaag
     1561 ccacacccag cgcgacggaa tattttaaga aatcacgacg ttgcatagtt atttccttat
     1621 tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta cgggaaggtc
     1681 aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac
     1741 gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa cagtgttatt
     1801 cggcagcctg atggccgtct gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga
     1861 tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt accagaactt
     1921 acctaacgtg caaattcgtc gcgcactggt ctttttcgct cagcaaaacc agtgggacct
     1981 cagtaattac gacaccttcg acatgaaagc cctcggtgaa gacagctacc gcgatctcag
     2041 cggcattggc attcccgtcg ctaaaaaatg caaagccctg gcccgcgatt ccttaagcct
     2101 gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc gtgcatccgc
     2161 ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa ggaggtatca
     2221 acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg ggcagtactt
     2281 tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga tcatttttga
     2341 gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca ccgagcgcga
     2401 cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccatg gtcacgcgaa
     2461 acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa
     2521 aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtatcgt tctgccgtca
     2581 gtatctaccc aaccataacg ccggtagcta cgacgatccg cgctttaagc tggtgatcga
     2641 cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta tctccgactg
     2701 caccgatcct atcggtcccg gcgaaagcct tttcacttcg gcattttatg aaggctgcaa
     2761 acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca
     2821 ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg ttggctttta
     2881 tcaggcggcg atcccgacct attacggcgg tatcatgact tttgcatggg cgacagataa
     2941 cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg cttctggcct
     3001 gaaatgccgt tattacaatt cggcaatcca tacggcagct tttgccttac ctcagtatct
     3061 gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga aaaaactgaa
     3121 actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg atatctgcta
     3181 cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac tctataatgc
     3241 caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca atattcttaa
     3301 catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg tgagtgaaga
     3361 accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc cagaaacggt
     3421 ggttgcccat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa gtcatcctga
     3481 aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg tgatttctcc
     3541 gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa ccattgatta
     3601 tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag cactttatcg accatgagat
     3661 taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt
     3721 gaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt tcgaccttaa
     3781 gcactacatg ttccacacaa aaccggaaga cttaaccgac agcgagcgcc aggaaattac
     3841 cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc cagctgttta
     3901 acgtctcggg cggagctccc aggctccgcc agatttattt acttctgctg cacgaaatta
     3961 cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag gctttcttcg
     4021 tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg attggcgcga
     4081 accatcacct cttcaccgtc catccacagg gtgtattcat gccctgcccg ttgccaggac
     4141 cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa ggccaggttt
     4201 tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga catacgcacc
     4261 tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc ctctgcaccc
     4321 ggcaatgcgc cgaaatcgca ttgcgccatt gatgtaatta tcgcagcctt accggagaaa
     4381 aacagcggaa cggcgatggt ttagaaataa aaaatgcccg ggaatctcat tcaccgggca
     4441 ttgtgtcgtt tatgcgcagc gcgtgcgctg actttttaaa ctgcagtctg gaaaatcacc
     4501 ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg ataagtatca
     4561 acggcagttt tatctacttg cgtaacgtag gtctggtact cttccggcgt cggcagtttg
     4621 ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt cgcgccagtc
     4681 cccagacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc gtctgctaca
     4741 cgcgcctggt tacccataca cagagaacag cccgggatct cgatacgagc cccgctctta
     4801 ccgaagacgc tgtagtagcc ttcttcagtc aactgtgcgg cgtccatacg ggttggcggt
     4861 gccacccaca ggcgggtcgg caactggcct ttatgcgcat ccagcagttt acccgcagca
     4921 cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat cttctcgccc
     4981 tgtaccgcag acagcggacg cgcgtcatcc gggtcgttcg gagcacacag gattggctct
     5041 ttaatatccg ccagatcgat gtcaatcact gccgcgtatt ccgcatctgc atcggcttcc
     5101 agcagctcag gattcgccag ccatttttcc atgccctgaa tacgacgttc cagggtacga
     5161 cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttaga gttaaggtat
     5221 tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg ctcagcggac
     5281 gcatcggtaa gctcaaaggc ctgctcaact ttcagatccg gcagaccttc aatttcgagg
     5341 atgcggccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag accttgtttg
     5401 atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg
     5461 cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc agttgcggca
     5521 gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa acgggtatgg
     5581 gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca ggagtggatt
     5641 acgccgtccc ccggacgcag cgacacaccg ccacggttca taatgaagtc cggcagcgtg
     5701 tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa agactgcatc
     5761 accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg ggtcatcggg
     5821 cctgtggtgt cctgagagcc tacagaagtc attttcggtt cacagtacgc gcccggacga
     5881 atgcctttca cgccacaggc acggcctacc attttttgcg ccagcgagaa gccgcgatcg
     5941 ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc aagtgcttca
     6001 cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc agcacgcact
     6061 tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc tgtttcgtgg
     6121 ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt caggttagag
     6181 acgtcgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca
     6241 attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg aatatcatcg
     6301 cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt acccacaacg
     6361 tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg tttgatcggg
     6421 ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag catcgccagc
     6481 gcgtgcagtg ggatatccgg gcgcgaccac gcatccggtg ccggagaaag gtcatcggtg
     6541 ttggtttcgc cagtgacttt gaagacggta acggtcagtt tttcagccag cgccgggcga
     6601 ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata ttcgttgcct
     6661 gctttggctt tctcttctac gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt
     6721 gctttggcag cgatcggtgc cagtttggca tcatccagcg cgtcgatcag cggatgaatg
     6781 ttgtaaccac cctgcatggt acccagcagt tcgatggctt tttccggagt cagcagaggg
     6841 gatttggctt cgcctttagc gatagcagcc aggaagcctg ctttgacata ggcggcttca
     6901 tcgacgccag ggggaacacg gttggttaat agatctaaca ggaattcttc ttcgcccgcg
     6961 ggcgggtttt tcagcagctc tacaagtgcg gccatttggt ttgcatccag gggtttgggc
     7021 gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag cacgacggtt
     7081 ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct gtgagacagc
     7141 agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt tgacgacgtg
     7201 ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg atggtctaga
     7261 gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc tttctcaacg
     7321 aatcccaaga ttagtaaaaa tttaaactac cgcctcttta tactcggatt cacagcacct
     7381 gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg aaaatgactt
     7441 tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt gccacctcta
     7501 ccgggctata tataaaaagc cgcacagtgg aagcgcctgt ggaaccgcaa tcgacacaac
     7561 tggctgtgtc tgacgctgcc gcggttacgc ttcctgcaac ggtttccgca cctcccgtaa
     7621 cacccgccgt cgttaaatcc gcattcagca ctgcacaaat agatcaatgg gtcgcgcccg
     7681 tcgcgctata ccccgactct ctgctttcac aagtgttaat ggcatcaacc tatccggcaa
     7741 acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc gatgctgcta
     7801 ttcaggcggt atctgaccaa ccgtgggacg ctagcgtcaa atcactggtg gcctttccac
     7861 aattgatggc gttgatgggc gaaaacccgc aatgggtgca aaacctgggc gatgcttttc
     7921 tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg gcgcaacaaa
     7981 ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag aaagctgtac
     8041 cggtaaaaca gacagtcacg gctcccgtca taccatccaa taccgtttca actgccaccc
     8101 ctgtcattac agagccggca acaaccgtca tttccattga gcctggcaat cctgatgtgg
     8161 tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc
     8221 cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt gtgcgcggat
     8281 tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc gactgggatg
     8341 acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac gatggcggtc
     8401 atcgtgacgg taatggctgg caacacaacg gcgacaacat caatatcgac gtcaacaatt
     8461 tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg cacaatccaa
     8521 actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt catcaaaccg
     8581 atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac agccagcgtc
     8641 aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt acacaagata
     8701 cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg agctatgacg
     8761 acttccgcga cttcagccgt cgccaaccac tgacccagca acaaaaggac gccgctcgtc
     8821 agcgttatca gtcggcctcg cctgagcagc gccaggcagt ccgcgagaaa atgcagacta
     8881 acccgcagat ccagcagcga agggaggcgg cgcgccagcg tattcagtcc gcctcgcctg
     8941 agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaagcag cagcgaagag
     9001 aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg tttaaggaaa
     9061 aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag cgtatacaat
     9121 cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc cgcccacaac
     9181 gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca gcagtacgcg
     9241 aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattacaggc gtaaaaaaag
     9301 cggcgtggtt agccgctttt ttaattgcca gatgtcccgg caaacgaaaa attacttctt
     9361 cttcgctttc gggttcggca ggtcagtaat gctaccttcg aacacttctg ccgccaggcc
     9421 cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt cagcatcaca
     9481 acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttag taccgacaat
     9541 cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca taccgtctgc
     9601 gcagtcggaa gcgatcgcac gaccagaagc agcccacggg aaggtggcgg tttcatagct
     9661 gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt ctggttcggt
     9721 ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg cgataacttc
     9781 agcggcaacg tgaccttcgt gaacaccttt atgtgccagc atcggctgac cgacgatatc
     9841 gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc
     9901 acggtcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg
     9961 accaatcgct accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc
    10021 catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg tttccagcat
    10081 caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt tgtcagctgc
    10141 cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg cgtggtatac
    10201 ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt ctggtacttc
    10261 tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg gaataaacgg
    10321 cagttggatc gggcgagaac ccgctgcaat gatcgcattg tcgaagttga tcacggtttt
    10381 gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca gaccgttgac
    10441 cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct gattgatcac
    10501 tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga agacgatacc
    10561 gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca gtgctttaga
    10621 agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac
    10681 ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc
    10741 aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat atttatctcc
    10801 ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag
    10861 ttacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg cccccagaaa
    10921 tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc cgtcggtatt
    10981 ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct attcgctaat
    11041 aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg gctcttttac
    11101 ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc
    11161 accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca gcatcagacg
    11221 cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa cgccgaggat
    11281 agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc cgccgatgct
    11341 ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt
    11401 cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt
    11461 gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt tgatgtattt
    11521 cttcagggtc agacgctgac cgtcttccga cagcgaacta ttgaagcgag gcatctgctc
    11581 aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca catccagctt
    11641 acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccaactcgg tgatatcggt
    11701 tttgtcgaag tgagtaacat gcgggatcat tacccagtta cggctcaggt tcgcaccaga
    11761 gattttctgg atgcggccca gttccacttc ttcgatttca ccaaacttgc tgaagtccac
    11821 cttcggccac ggcagcatgc cagggatacc accgccagtc gctgccggag ctgcttctgc
    11881 acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac gacctttacg
    11941 gccagtgccc ttcactttcg caaggttaac accaaactcg cgtgccagac ggcggatcag
    12001 cggagtcgcg tgaacataag cgtcgttttc agcaaattca gatttgcctt ccgctttcgc
    12061 agctggtgct gctgccgggg cttcagcttt tgctgccggt gccggcgctg ccgcttcctg
    12121 tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc
    12181 agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg caaacggcgc
    12241 cggaacttcc atagaagctt tgtcgccttc tacggtgatc agtgactgtt cagcggcaac
    12301 tttgtcgccc actttcacca tcacttcagt cacttcaact tcgtcaccgc cgatatccgg
    12361 aacgttaact tctttcacgc cagccgctgg tgcaggggcc gctgccggag ctgcttcctg
    12421 tttagcggcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca taatcagcga
    12481 gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtgc cagcaaacgg
    12541 agccggaact tccatagaag ccttgtcgcc ttctacggtg atcagcgact gttcagcttc
    12601 aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc tgccgatatc
    12661 cggaacgtta acgtcttttg ccgccgcagc cgctggtgct gctgccggag ctgcttcttt
    12721 cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat
    12781 aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt taacgatacc
    12841 cgcctgcgga gacggaactt ccatagaggc
//
LOCUS       DACAPB010000134        12528 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.134, whole genome shotgun sequence.
ACCESSION   DACAPB010000134 DACAPB010000000
VERSION     DACAPB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.134"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            371..541
                     /locus_tag="IT904_003339"
     CDS             371..541
                     /locus_tag="IT904_003339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193078.1"
                     /translation="MKYFTVEQVVEALKTGAARRHQIYDNFAQARYRGFTERAALFKT
                     ALDIFDQWKKEN"
     gene            671..1096
                     /locus_tag="IT904_003340"
     CDS             671..1096
                     /locus_tag="IT904_003340"
                     /inference="COORDINATES: protein motif:HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193079.1"
                     /translation="MNKNEPPFLAIDYRILALDIKAAINKKTIDSSTILKLKLVYCYL
                     LGFSKTCEEVYPEQTKMGKKLGIGSRQVVGRCISLLESLGWISKTSRTGTSNLYTVKT
                     VEQILEGIPREVKEEEEEKEFHDFNTGIVLIEDDDDLPW"
     gene            1112..1474
                     /locus_tag="IT904_003341"
     CDS             1112..1474
                     /locus_tag="IT904_003341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193080.1"
                     /translation="MAYARQNVVFEETTEIGFNSRTKKVKIPKKLKPLAIIKLYESSP
                     ALSEDEQSIINSMQGVKWKPRQRYTGFKFNRDPLKFEYLFYNINEYEGWLEFNRLENE
                     NFTGNDLGLDFDAPGISD"
     gene            1500..1775
                     /locus_tag="IT904_003342"
     CDS             1500..1775
                     /locus_tag="IT904_003342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193081.1"
                     /translation="MINFEQHKNIVEDFVEQHYPLAHSLMIDSYKDAEVYYSNFQILM
                     REMNTLPENPEYFLEWLLEDDPALYINLMELIVIARTIHNVFEQVSP"
     gene            1857..2117
                     /locus_tag="IT904_003343"
     CDS             1857..2117
                     /locus_tag="IT904_003343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193082.1"
                     /translation="MKYDENSIRVLNQDELEQHSVDSILSILLDENPDISPDHLAKMA
                     ECLVTLGYGVTEYREYKDGTLDNIKRQTMQEWFRERNKIKKY"
     gene            2468..2671
                     /locus_tag="IT904_003344"
     CDS             2468..2671
                     /locus_tag="IT904_003344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193083.1"
                     /translation="MVTITKGVKTFDGKIPVTLLVDDVETANELIKLAKAANVPVVEQ
                     EDVLNKSTINPVFKHLDRIASNK"
     gene            2810..3142
                     /locus_tag="IT904_003345"
     CDS             2810..3142
                     /locus_tag="IT904_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193084.1"
                     /translation="MRDTVVAYVSDGVKQDELVKLSAAIPADLPVGSVVDTTGTLITT
                     AADADYVVVEFAKAGSTAIVVANPYHTILKKSGLITNGVDLSSVVEQLQALHHSFADF
                     ATVALRTT"
     gene            3183..4202
                     /locus_tag="IT904_003346"
     CDS             3183..4202
                     /locus_tag="IT904_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein E"
                     /protein_id="HAP3193085.1"
                     /translation="MPFDTRNNAFEYASPFEYSNEPNLIISNLGLFEEENHTKNSILL
                     DKILIESNETAPEKMPYAQSDWNSAQRSDYTSVLYDMVYTNSLNTVSSRDIELLRAPG
                     SDHEENIAMVLAEYSQRQYSQISNTVEQYLIDALFGLKVESQYAGQGNIDFLAGNAKT
                     SYVMNVANTANVFEQLDEVNRIINVKLGSGLAKKRKGTVILAAGNAAKALKYHQSIKD
                     YMVYTLSLNDSDNFATKIVSQNPAFSSWKLDTVNVIDVTGYSELTDYIGENGFAVVPM
                     VDGGFKLHTGIGVRHSRLGREPGLVHQYMTDVDRMGWPAIHTESAFLPQVNIPDSIVF
                     GSISS"
     gene            4306..4503
                     /locus_tag="IT904_003347"
     CDS             4306..4503
                     /locus_tag="IT904_003347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193086.1"
                     /translation="MAKIELTDSQGNKYSLDVTDGDLFRIFNITKLSSVVGINKYTLS
                     ARVCRQYPDGEYRSLVTALNK"
     gene            4567..6387
                     /locus_tag="IT904_003348"
     CDS             4567..6387
                     /locus_tag="IT904_003348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193087.1"
                     /translation="MSGSKVTAATLVNRVVYKTDLNSLKKVRNDMKKLQQEFSKSNNQ
                     VAKVKQQADKQIHQQQLKQQREIDKQQKQAAKQAAVDAKAKANEQKKLAAAQARALKI
                     QQQQTKKVATQQENADLARKRAAFQLGRLQGMDGASRYQAIKQANAIVDAYARGNQSL
                     KSMNQALSQHLTTQRAISRNNAKRAKQSGKGKKSGSSGWLSRQASAIIPGEILGPTAA
                     AYAAQQAYQIISGTLQGAIERNQGRKMISSMGLNPLEADAAIQETLKRTGFNMSYEKI
                     SDIAKDTQDKIGQLSLGQWTKDKQGKASFSGGGEMSDWLQIMVNRGGYSQEGALKTLQ
                     NIKGPVELAVLLKSLQKSAKLTESEFTAFAEVINDFSYLAKSVDASGQNVIDTMNTMV
                     NSGLALNSQQQKSIDNLSQMSIVFDNARNALGDQFAASFSKGLDDLGINTQNLSSEMG
                     SLVPLVKDLGTGTAQTVKGFKDLLDVLSIISEWRNRLLGEKPEDRNILQQGATFVGES
                     LLPPGVLDMASRIKNIVSPGSDSSVASQVQSGGWYNQMMVQTPAMQYQPVIPDLKADF
                     NLTVNPSPEFGMLVEAVADKRIEWAFDDQNFQINQSILGQ"
     gene            6710..7177
                     /locus_tag="IT904_003349"
     CDS             6710..7177
                     /locus_tag="IT904_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193088.1"
                     /translation="MENHNFERLKEHILSLSVSKNFEVAKTEWKLHSIEVTEELDSCP
                     CGQDIKEHCYIENKKNGNRTYVGNKCINRFLNISTGTLFDGLRRIKENIYANPNLALI
                     EYANDKGYLYDKEYDFLVDTMKKRNLSDKQKLWKEKINNRILKEIVVKSRTAR"
     gene            7456..8331
                     /gene="rluB"
                     /locus_tag="IT904_003350"
     CDS             7456..8331
                     /gene="rluB"
                     /locus_tag="IT904_003350"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAP3193089.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(8371..8961)
                     /gene="cobO"
                     /locus_tag="IT904_003351"
     CDS             complement(8371..8961)
                     /gene="cobO"
                     /locus_tag="IT904_003351"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP3193090.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(8958..9716)
                     /locus_tag="IT904_003352"
     CDS             complement(8958..9716)
                     /locus_tag="IT904_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAP3193091.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            9936..10985
                     /gene="sohB"
                     /locus_tag="IT904_003353"
     CDS             9936..10985
                     /gene="sohB"
                     /locus_tag="IT904_003353"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAP3193092.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRVIDRLTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(11021..11272)
                     /locus_tag="IT904_003354"
     CDS             complement(11021..11272)
                     /locus_tag="IT904_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAP3193093.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            11652..>12528
                     /locus_tag="IT904_003355"
     CDS             11652..>12528
                     /locus_tag="IT904_003355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP3193094.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQA"
BASE COUNT         3702 a         2597 c         2967 g         3262 t
ORIGIN      
        1 ccccacaggt ttacacagcc tacagaccct gtagaaaagc cctacaggcg atttaaatga
       61 taaaaatgat aatggtatgt ctaaagtaag aaaacctctc agaacgcgat acagagcgtt
      121 ttagaaggta tctaactgcg tacgcaggtt aggcatcata aagtgaatct ttattcttac
      181 agggattgac accagcacag ggagtttgat attattcgat acgtaaaaag atatcttaaa
      241 aatgcattga cagagttcta tcattagtat agaatagtag taggaaacag aaaaacctaa
      301 aagcaaagca ctttgagcca ttccttggct gcaaggtgta attttattta tccctaccca
      361 acggaggcct atgaaatatt ttacagtaga gcaagtagta gaagcattaa aaactggcgc
      421 agcacgccgc catcaaattt atgataattt tgcccaagcg cgatatcgcg gatttactga
      481 acgcgcagca ctttttaaaa ccgcgcttga tattttcgac cagtggaaaa aggagaatta
      541 agaatcatga acattcgttt tagccctgac actcacaaac ttttaatagc tcgtgccaat
      601 cgtgaggata agcccgcagc cgcactggtt aacgaattaa ttacagcaat cctaaagcaa
      661 gaggaaaaga atgaacaaaa acgaaccacc atttctggca attgattacc gcatcctggc
      721 attagatatt aaagcagcca tcaacaaaaa aaccatcgat agcagcacaa ttctcaaact
      781 taaactagtt tattgttatc tcctggggtt ctccaaaact tgcgaggagg tttatccaga
      841 acaaacaaaa atgggtaaaa aattgggtat tggttcccga caggttgttg gaagatgcat
      901 atcgctgctg gagtctcttg gatggatttc aaaaacatcc agaactggaa catccaatct
      961 atacacggtg aaaaccgtcg agcaaatttt agaaggtatt ccgcgagagg ttaaagaaga
     1021 ggaagaggaa aaggaatttc atgattttaa tacaggaata gtattaattg aagatgatga
     1081 tgatttacca tggtaaacaa caggaggaat attggcatat gcacgtcaaa atgtagtatt
     1141 tgaagagacg acagagattg gatttaatag cagaacaaaa aaagtaaaaa tccctaagaa
     1201 gttaaaacca ttagcaataa tcaaacttta tgaatcatca ccagcattat cagaagacga
     1261 acaatcaata attaattcca tgcaaggtgt taaatggaaa cctcgccaac ggtataccgg
     1321 attcaagttc aatagagatc cgctcaaatt tgaatatctc ttttataata taaatgagta
     1381 tgaggggtgg cttgagttca atagactcga aaatgaaaac ttcacaggaa atgatcttgg
     1441 acttgatttt gatgcaccag gcatctctga ttaacaataa taataagagg taaacaacaa
     1501 tgattaattt tgaacagcac aagaatattg tagaagattt tgtagaacag cattacccac
     1561 tggcgcatag tttgatgatt gatagttaca aagatgcaga ggtgtactat tcaaattttc
     1621 aaattctcat gcgggaaatg aatacacttc cagaaaatcc agagtacttt ttagagtggc
     1681 tactggaaga tgaccccgcg ttgtatataa acctgatgga actgattgtc atcgccagaa
     1741 ctattcataa cgtgtttgaa caagtttcgc cttaacttta ccacctaaat ataaaaacct
     1801 ctcagaatgc gatacaggcg ctctgagggg catatttgaa cacaatggag gatagattga
     1861 aatatgacga aaacagcatt agagttttaa atcaggatga gttagagcag cattcagtag
     1921 atagcatctt aagcatcctg cttgatgaga atccagatat ttcccctgat catctggcaa
     1981 aaatggctga gtgtctggtt accttgggat atggtgtaac tgaatacaga gaatataaag
     2041 atggcacttt ggataatatt aaacgccaga caatgcagga atggtttaga gaaagaaata
     2101 aaatcaaaaa atattaaaaa tatctctatt tagagtctct atttattgac aaaataatgt
     2161 tttaatgtta ttataaatat gtagacaata atcaacggac gctatagcta ggacattgtt
     2221 tacagagaca taggcgttat tggcattacg tcttccttgg gaatccctat tattgctccg
     2281 ggcctagttc acccaccacg atagataatg gggcaattct caatatatat ttttcgtgag
     2341 tgtatattgt gaattgttct tccaaaagtc agttcaagtt aatcaatttt ctcttctgta
     2401 tcaaggccac gctaaatagt ggcctttctt ttatttcaat ttgcaaaatg aaatggattt
     2461 aaaaattatg gtaactatca caaaaggcgt caaaacgttt gacgggaaaa tccccgttac
     2521 gctgttggtt gatgacgtcg aaacggctaa cgagctaatc aaactcgcca aagctgcaaa
     2581 tgtccctgta gtagaacagg aagatgtttt aaataaatct acaatcaatc cggtgttcaa
     2641 acatcttgat cggattgcat ctaataaata ataacgtagc cgactaaacg tcggtgaaca
     2701 ttcgttcatc tgcggagtta acccgcagac taataaataa taaataggag acctaacgat
     2761 gtcttttttt gaaaattatg gtgaaacttt ttcgggtcgc cgtggatacg tgcgtgatac
     2821 cgttgtagcg tatgtgagcg acggtgttaa acaggatgaa ctggttaaac tgtccgctgc
     2881 tattcctgct gatttgccag tgggttctgt cgtggatact accgggacac tcatcaccac
     2941 tgctgctgat gctgattatg tcgtggttga atttgctaaa gctggttcta ccgcaattgt
     3001 tgttgcgaat ccatatcata ccattttgaa aaaatcaggt ctgattacca acggtgttga
     3061 tttaagcagc gtcgttgaac agctacaagc tctacatcat tcgttcgctg atttcgccac
     3121 tgttgcgcta cgcacaacct gattaatcaa tcccataact attataataa agaggataaa
     3181 acatgccttt tgatactcgc aacaatgcat tcgaatatgc atcaccattt gaatattcca
     3241 acgaacctaa cctgattatt agcaacctgg gattgttcga agaagaaaat catactaaaa
     3301 actcaatcct tctggataaa atcctgattg agtcaaatga aacagcacct gaaaagatgc
     3361 cttacgctca aagtgactgg aactccgccc agcgctctga ctataccagc gttctctatg
     3421 acatggtgta taccaacagc ctgaatactg tttcaagtcg tgatatcgag ttgctccggg
     3481 ctccaggttc agaccatgag gaaaatattg caatggttct ggctgaatac tcacaacgtc
     3541 agtattctca aatttctaat acagttgaac aatatctcat tgatgctctg tttggcctta
     3601 aagttgaatc tcagtacgca ggacagggaa acattgattt cctcgcaggc aatgctaaaa
     3661 cttcctatgt gatgaatgtt gccaatactg ctaacgtctt tgaacagtta gacgaggtaa
     3721 accgcatcat taacgttaag ttaggttctg gtctcgctaa aaagcgtaaa gggactgtta
     3781 ttttagctgc tggcaatgct gctaaagcgc tgaagtatca ccagtctatt aaagattata
     3841 tggtgtacac tttgagcctt aatgattctg ataattttgc tactaaaatc gtttctcaga
     3901 atccagcatt tagttcctgg aaactggaca ctgtaaacgt tatcgacgtc accggatatt
     3961 ccgaactgac tgactatatt ggtgaaaatg gttttgctgt tgtgcctatg gttgacggcg
     4021 gatttaaact acatacgggg attggtgttc gtcatagtcg tttaggtcgt gagcctggtc
     4081 tcgtgcatca atatatgact gatgtagacc gtatgggatg gcctgctatt cataccgaaa
     4141 gcgcattcct gccacaggtt aacattcctg attcaattgt atttggttct atctccagct
     4201 aatgatattc acggcagcgc taatactgct gctgtttgag tattgggttg gtcagcagca
     4261 aataccagcc catatcctat taatatttct ctggagatta aacaaatggc aaaaatagaa
     4321 ctaacagatt cccagggaaa taagtactcc ctggatgtta cagacggcga cctattccgc
     4381 atttttaata tcaccaaact ttccagcgtg gtgggtataa ataagtacac gttaagtgca
     4441 agggtatgcc gtcaatatcc cgatggtgaa tatcgaagtc tcgttacagc attaaacaaa
     4501 taaaaataaa ttataaaagc ctctacccgt caagggcggg gcttttttta tatggagcaa
     4561 ataaaaatga gtggaagtaa agtaaccgcc gcgactttgg tcaacagggt tgtctataag
     4621 acagatttaa acagtcttaa aaaggttaga aacgacatga agaaactcca acaggaattt
     4681 tccaaatcaa acaaccaggt agcaaaggtc aaacagcagg cagataaaca aattcatcaa
     4741 cagcaattaa aacaacagcg tgaaattgat aaacaacaga aacaagcagc taaacaagct
     4801 gctgtagatg ctaaagcaaa ggccaacgag cagaagaaat tggcagcagc gcaagcacgc
     4861 gctttgaaaa ttcaacagca gcagacaaag aaggtggcaa ctcagcagga aaatgctgac
     4921 ttagcaagaa agcgtgcagc cttccagctt ggacgcctcc agggaatgga tggagcctcc
     4981 agatatcagg caataaaaca agctaatgcc attgtggatg cctatgcacg aggaaatcaa
     5041 agtctcaaat cgatgaatca agctctgagc cagcatctga ctacacaacg cgcgatatcc
     5101 cgtaataacg caaaacgagc gaagcaatca ggtaagggaa agaaatctgg ttcatctggt
     5161 tggctgagtc gccaggcaag cgcaatcatc cccggtgaga ttcttgggcc aaccgcagcc
     5221 gcatacgccg ctcaacaggc atatcaaatc atctctggaa cactacaggg agccattgag
     5281 agaaaccagg gcaggaaaat gattagttct atggggttaa atcctctgga ggctgatgca
     5341 gccattcaag agacgttaaa acgaaccggc tttaatatga gctatgaaaa gatttctgac
     5401 atagcaaaag atacccagga caagatcggg cagttgagtc tcggacaatg gaccaaagac
     5461 aaacaaggaa aggcatcatt cagcggtggt ggtgaaatgt cggactggct gcaaataatg
     5521 gttaaccgtg gggggtattc tcaagagggc gcattaaaaa cgcttcagaa tataaagggg
     5581 cctgtggagt tagctgtatt attaaaatcc cttcagaaga gcgcgaaact aactgaatca
     5641 gaattcactg cgtttgcgga agtaataaac gacttttctt atttagctaa atctgtcgat
     5701 gccagcgggc aaaacgtcat tgatactatg aacacgatgg ttaacagtgg tctggcgctt
     5761 aatagccaac agcagaaatc tatcgataac ctctcgcaga tgtcgattgt attcgataac
     5821 gccagaaatg cattaggtga tcagtttgcc gcatctttta gtaaaggtct tgatgattta
     5881 ggaattaata ctcagaatct atcttctgaa atgggttcac tggttccgtt ggttaaagat
     5941 ttaggaacgg ggacggctca aacagttaag ggatttaaag atcttcttga tgtattgtcc
     6001 attatttccg aatggagaaa tagactttta ggtgagaagc cagaagacag gaatatcctt
     6061 cagcaagggg ctacgtttgt tggggaatca ctgttgccac cgggcgtcct cgatatggca
     6121 agccggatta aaaatatagt tagccctggt tctgattctt ctgttgcatc ccaggtgcaa
     6181 agtggtggtt ggtataatca aatgatggtg cagaccccag ctatgcagta ccaacccgtc
     6241 attccggatt taaaagctga ttttaatctg acagtaaatc cgtctcctga atttggaatg
     6301 ctggtggagg cggtagcaga taagcggata gagtgggcat ttgatgatca gaacttccaa
     6361 attaatcaaa gtattcttgg tcagtgatat gtctgttttt agcatatctt gtgctacttt
     6421 gtgatttgca ttgcacatat agtaatctcg tgttgctgcg gcttcgctaa tcacctatgc
     6481 ttgaagaggt tatagtagtt cgcccattca atcttgatga ggtgttttat ggctaaacaa
     6541 aaagttgttc gtgatgcagg tactggtcag tttgttaagc ctgaagaggc taagcgccgc
     6601 cccggtacta cggttacaga aactgttaag attccttcca aaaaagggaa aaagtaaaac
     6661 atcttgaagc gcctttagtg gcgctttttc tctctctgga gatcttttga tggaaaatca
     6721 taactttgaa agactcaaag aacatatctt atcgttatct gtttcaaaaa acttcgaagt
     6781 tgctaaaaca gagtggaagc tacattcgat agaagttact gaggaactgg attcctgtcc
     6841 atgtggccag gatatcaaag agcattgcta tattgaaaat aagaaaaatg gcaataggac
     6901 ttatgttggt aataaatgta taaatagatt tcttaatatt tcaaccggaa ctttatttga
     6961 tgggttgaga agaattaagg aaaatattta tgcaaatcca aacttggcat tgattgagta
     7021 tgcgaatgac aaagggtatt tatacgataa agagtatgat tttcttgttg atacgatgaa
     7081 gaaaagaaat cttagcgata agcaaaagtt gtggaaggag aaaattaata atcgcatcct
     7141 taaagaaatt gttgttaaga gtcgtacagc aaggtaagtg ttctttttta gcaactttaa
     7201 ttcggaagca atgacagcca gcacgttcta tgaaaaaagt aataaataag agaggggcat
     7261 tacgcccctt tttcatgtct aaaattcgag ttagagctaa acttgtttaa acctatgtat
     7321 tgtacaacaa actgggttac aaactggtga aaagtgcgta taatagcttg caatgaaatg
     7381 tgctaacgca ttgatttata tagatttata taaaatatcc cgacgcctgg gaaggcgaca
     7441 ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc cggccacggt
     7501 tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga tggcaaaatt
     7561 gctaaactcg gcgatcgtgt tgaagttacc cctggattga aaatccgtat cgatggtcac
     7621 ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta ttacaaaccg
     7681 gaaggtgagt tgtgtacccg taacgatccg gaaggacgtc caacggtgtt tgaccgtctg
     7741 ccaaaactgc gtggcgcacg ctggattgcc gtgggtcgct tggacgttaa tacctgtggt
     7801 ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcaccc aagccgtgaa
     7861 gttgaacgtg aatatgccgt gcgtgtattt ggtcaggttg acgacgcgaa actgcgtgat
     7921 ttgagtcgtg gcgtgcagtt ggaagatggt ccggcagctt ttaaaaccat caagttcagc
     7981 ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg taaccgtgaa
     8041 gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg tgttcgttac
     8101 ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct ggacctcgcc
     8161 cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc taaagtcgct
     8221 gtagaaaaag accgtcgtcg catgaaggcg aatcagattc gtcgtgcggt gaaacgtcac
     8281 agtcaggtga gcggcggtcg tcgttctggc ggacgtaata ataacggtta atcaaagtat
     8341 gccggacgtc atatccggca tttttacaga ttaataatcg atccctatct gcgcttttac
     8401 accggcatca aacgcatgtt tgacggggcg taattcactt accgtatctg ccagttcaag
     8461 aatatcccga tgacaaccac gacccgtgat aatcaccgtc tgttgatgtg gacgttcatt
     8521 taacgcctgc accacttctt ccagtggcaa atagtcatac gccaccatat acgtcagttc
     8581 atcaagcaaa accatatcca gtgaggaatc agcaagcatc cgctttgcat gttgccagac
     8641 ttcgcggcag gcggcggtat cagactcgcg gttttgtgta tcccaggtaa agcccgttgc
     8701 catcacctga aactcaacgc catgtggctc cagcagattg cgttcgccat taggccaggt
     8761 gcctttaata aactgcacga cgcctacttt ttttccgtga ccaactgcgc gtgttgccgt
     8821 accaaatgcc gcggtggttt tgccttttcc attgccggta aagacgatga taataccgcg
     8881 ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct gctgacgctg
     8941 ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg ggcgtcaaag
     9001 gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag cggcatgata
     9061 tcagcgggtg ttttaagttt ctgtggatct tcggtcggga aggcgctggc acgcattgcg
     9121 gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc gctgctgata ttcatcggcc
     9181 agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc ccagttggct
     9241 cgtccctgac gtccaacgct tgatgaagta aagaccagtg aaccggcgtc cgatttgagt
     9301 aataaaggaa gcagtgcctg ggtgagcata aaggtggcat taacgttgac ctgtatgacg
     9361 tcctgccaga cctgtggatt ttgttcgctc attggacaaa catcgccgag caatccggca
     9421 ttatgcaaaa caccatccag acgcggataa ttaacggcaa tgcgctgtgc cagttgttgg
     9481 caatcttcgg acctgcaggt cagcaaatcg agaataaacc actgtggctg acgcccagtt
     9541 tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc caacagaatc
     9601 actgtcgcac catagcgtgc atacgtcatc gcggcttcac gaccaatacc atcactggct
     9661 cccgtcacca ggataatgcg atcattgagt aaatcttgtt ttggctggta atgcatggct
     9721 actcctcaac gacgttgtct gtcgcgctgc cagtgtactt tatgactttg gggctttatg
     9781 cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa tggatacttt
     9841 gtcatacttt cgctgcaata acatctctgc gagacggctt aacatgcctg ttgtaaactg
     9901 tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct gaatatggtt
     9961 tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc gccattattg
    10021 tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acgggtcaac aatctcagcg
    10081 aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggactca catcagcaaa
    10141 aacagtggca caaagcgcag aagaaaaagc ataagcaaga agcgaaagca gcaaaagcga
    10201 aagccaaact gggagaggtg gcaactgaca gtaaaccccg cgtctgggtg ctggatttta
    10261 aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg gctgtactcg
    10321 cagcattcaa accgcaggat caggttgtac tacgtctgga aagccctggt ggcatggtgc
    10381 atggttacgg gttggcggct tcgcagctgc agcgtctgcg tgataaaaac attcctttaa
    10441 ctgttacggt agacaaagtc gctgccagcg gcggttacat gatggcctgt gtggcggaca
    10501 aaattgtttc cgcaccgttt gctattgtgg gttccattgg ggtggtggcg caaatgccca
    10561 actttaaccg cttcctgaaa agcaaagata ttgatatcga acttcacacc gccgggcagt
    10621 ataagcgtac gctgaccttg ctgggtgaaa ataccgaaga agggcgggag aaattccgcg
    10681 aagagctgaa cgaaacgcat cagttattta aagattttgt gaagcgtatg cgtccgtctc
    10741 tggatattga acaggtggca acgggtgaac actggtacgg acaacaggcg gtagagaaag
    10801 gcctggttga tgaaatcaac accagtgatg aagttattct tagcctgatg gaaggccgtg
    10861 aagtggtcaa tgtacgctat atgcagcgta aacgagtcat tgatcgactc accggcagcg
    10921 cggcagagag cgccgatcga ttgttgttac gctggtggca gcgggggcaa aagccattga
    10981 tgtaaaagac aaacgcgagg gtaagacctc gcgttttgct ttaatcaacc aggtgatatt
    11041 tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg cttttggctg
    11101 ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa taccagcacg ccattacgct
    11161 gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga atgattttgt
    11221 tggcctcttt caacagcgtc tcgcggtcga tgggttgtgt ctctttgttc attatttact
    11281 ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat accacgctgg
    11341 tggcaagagc gccttactgg caactttgga ttttgcatgc taataaagtt gcgtatcgga
    11401 ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct tgacattcga
    11461 ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca gtcacctgaa
    11521 ttttcgtgaa cagagtcacg acaaggggtt gatatccgca gagagcgagt ccatatcggt
    11581 aactcgttgc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga atcaaattag
    11641 gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa gccaaaacga
    11701 tcaacaagta tctgggtagt gactacgtgg tgaaatccag cgtcggtcac atccgcgatt
    11761 tgccgaccag tggctcagct gccaaaaaga gtgccgactc tacctccacc aagacggcta
    11821 aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt gacccgtggc
    11881 acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc tctgaactga
    11941 aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac cgcgaagggg
    12001 aagccattgc atggcacctg cgggaagtga ttgggggtga tgatgcgcgc tatagccgag
    12061 tggtgtttaa cgaaattact aaaaacgcga tccgccaggc atttaacaaa ccgggtgagc
    12121 tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc gtggtggggt
    12181 atatggtttc gccgctgcta tggaaaaaga tcgctcgtgg tctgtctgcc ggtcgtgtgc
    12241 agtcggtggc agtccgcctg gtggtcgagc gtgagcgtga aattaaagcg ttcgtgccgg
    12301 aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg ttggcgttgc
    12361 aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa actcaggctg
    12421 cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac aaaccgacaa
    12481 ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctg
//
LOCUS       DACAPB010000135        12419 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.135, whole genome shotgun sequence.
ACCESSION   DACAPB010000135 DACAPB010000000
VERSION     DACAPB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.135"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1144
                     /locus_tag="IT904_003356"
     CDS             <1..1144
                     /locus_tag="IT904_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAP3193095.1"
                     /translation="DAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLA
                     RQQKAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDE
                     RIWLGDYGSSLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFL
                     AARTIQDLPGKWQAQTSFSAEQLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDAS
                     GYPQSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVD
                     LLPAGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEY
                     QPVTLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            1145..3457
                     /gene="pbpC"
                     /locus_tag="IT904_003357"
     CDS             1145..3457
                     /gene="pbpC"
                     /locus_tag="IT904_003357"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAP3193096.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKITTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NSIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            3606..4037
                     /gene="ndk"
                     /locus_tag="IT904_003358"
     CDS             3606..4037
                     /gene="ndk"
                     /locus_tag="IT904_003358"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP3193097.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            4187..5341
                     /gene="trmG/rlmN"
                     /locus_tag="IT904_003359"
     CDS             4187..5341
                     /gene="trmG/rlmN"
                     /locus_tag="IT904_003359"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAP3193098.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            5626..6639
                     /gene="rodZ"
                     /locus_tag="IT904_003360"
     CDS             5626..6639
                     /gene="rodZ"
                     /locus_tag="IT904_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAP3193099.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            6666..7784
                     /gene="ispG"
                     /locus_tag="IT904_003361"
                     /gene_synonym="gcpE"
     CDS             6666..7784
                     /gene="ispG"
                     /locus_tag="IT904_003361"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAP3193100.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            7895..9169
                     /gene="hisS"
                     /locus_tag="IT904_003362"
     CDS             7895..9169
                     /gene="hisS"
                     /locus_tag="IT904_003362"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP3193101.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            9187..9807
                     /locus_tag="IT904_003363"
     CDS             9187..9807
                     /locus_tag="IT904_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAP3193102.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            9818..10996
                     /gene="bamB"
                     /locus_tag="IT904_003364"
     CDS             9818..10996
                     /gene="bamB"
                     /locus_tag="IT904_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAP3193103.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            11114..>12419
                     /gene="der"
                     /locus_tag="IT904_003365"
     CDS             11114..>12419
                     /gene="der"
                     /locus_tag="IT904_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP3193104.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYK"
BASE COUNT         2895 a         3069 c         3562 g         2893 t
ORIGIN      
        1 agacgccatt aaccggggta atgagcgtct gctgcgctat ttacaagatc cgggcatgat
       61 gtcgatcccg tacgcggata atctcaaagc cagtaaattc gccgtacagt cttacgctgc
      121 gctggtgttg gcccgtcagc aaaaggctcc gctgggtgcg ctgcgtgaaa tctgggagca
      181 tcgtgcagat gccgcttctg gtttaccgct gctgcaactt ggcgttgcgc tgaaaaccat
      241 gggtgatgcg acgcgtggtg aagaagcgat tgcgctggcg ctgaaaacgc cgcgtaatag
      301 tgatgagcgg atatggctgg gtgattacgg tagttcactg cgcgacaacg cgttaatgct
      361 ctccttgctg gaagaaaata aactgctacc cgatgagcag tacactttgc tgaacacact
      421 ttcgcagcag gcgtttggtg aacgctggct atcgacgcag gaaagtaacg cgttgttcct
      481 ggctgcccgt acgattcagg atttacccgg taaatggcag gcgcaaacct ctttctcagc
      541 tgagcagctg acaggcgaga aagcgcaaaa cagcaatctg aatagcgatc aacttgtcac
      601 cttgcaggtg agcaacagtg gcgatcagcc gttatggttg cgtatggatg ccagcggtta
      661 tccgcaatcc gcacctttac cggcgaacaa tgtgctgcaa atcgagcgtc atattcttgg
      721 tactgatggt aagagcaaat cgctggactc gttacgtagc ggcgatctgg tgctggtgtg
      781 gttgcaggta aaagccagta acagcgtgcc ggatgcgtta gtcgtggatc tgctgcctgc
      841 gggtctggaa ctggaaaacc agaatctggc gaacggtagc gccagcctgg agcaaagtgg
      901 tggcgaagtg cagaacttac tgaaccagat gcagcaggcg agcattaagc acattgagtt
      961 ccgtgacgat cgctttgtgg cggcggttgc cgttgatgaa taccaaccgg taacgctggt
     1021 gtatctggcg cgggcggtga cgccgggaac gtatcaggta ccgcaaccga tggtggaatc
     1081 aatgtatgtt ccccaatggc gggcgaccgg cgcggctgaa gatctgctga ttgtcagacc
     1141 gtaaatgcct cgcttgttaa ccaaacgcgg ctgctggata acgttggcag ccgcgccctt
     1201 tctccttttt cttgcagcgt ggggagcaga taaactctgg cctctaccgc tgcatgaagt
     1261 caatcccgca cgagtggtcg tggcgcagga tggtacgccg ctctggcgct tcgccgatgc
     1321 tgacggcatc tggcgttatc cggtaacaat cgaagatgtt tctccacgtt accttgaagc
     1381 gctgatcaat tatgaagatc gctggttctg gaagcatccg ggggtgaatc cattctcggt
     1441 ggcgcgcgca gcatggcaag atctcacttc gggacgggtt atttccggtg gcagcacgct
     1501 cactatgcag gttgctcgtc tgcttgatcc tcaccccaaa acatttggcg gcaaaattcg
     1561 ccagctctgg cgcgcgttgc aactggaatg gcatctgtct aagcgtgaaa ttctgacctt
     1621 gtatcttaac cgcgctccgt ttggcggtac gttgcagggg atcggtgcgg caagttgggc
     1681 ttatctcgga aaatcgcctg cgaatttaag ctattccgag gcggcaatgc tggcggtttt
     1741 gccgcaagcg cccagccgtc ttcgcccgga tcgttggccg gagcgtgccg aagccgcgcg
     1801 taataaagtg ctcgaacgga tggccgtgca aggtgtgtgg tcccgtgagc aggtaaaaga
     1861 gtcaagggaa gaacccatct ggctggcccc ccgacaaatg ccgcaactgg caccgctgtt
     1921 ttcgcgcatg atgctcggta aaagcaaaag cgacaaaatc actactacgt tggatgccgg
     1981 tcttcaacga cgtctggaag aactggcgca aaactggaaa gggcggttgc caccgcgcag
     2041 ctcactggcg atgatcgtgg ttgatcatac cgatatgcgt gttcgcggct gggtgggatc
     2101 ggttgatctc aacgatgatt cacgctttgg tcatgttgat atggtcaatt cgatccgatc
     2161 gccaggatca gtgctcaaac cgtttgttta tggtctggcg ctggatgaag gcttgatcca
     2221 cccggcatca ctgctgcaag acgtcccccg gcgcaccggt gattatcgac caggtaactt
     2281 tgatagcggt tttcatggcc cgatcagcat gagcgaggcg ctggtgcgct cgctgaactt
     2341 acctgctgtg caggtgctgg aagcctatgg accgaaacgg tttgcggcaa agttacgcaa
     2401 tgttggattg ccgttatatt tgcccaacgg tgctgcgccg aatctttcac tcattctcgg
     2461 cggcgctggt gcaaaactgg aagatatggc ggcagcgtat accgcgtttg ctcgccacgg
     2521 caaggcaggc aaattgcgct tacagcctga cgatccgctg cttgaacggc ctttaatgtc
     2581 gtcgggggcg gcgtggatca ttcgccggat tatggctgat gaagcgcaac ccttgccgga
     2641 tagtgccttg ccgcgcgtcg ccccactggc atggaaaacg ggcaccagct atggctatcg
     2701 tgacgcctgg gcgattgggg ttaacgctcg ctatgtcatt gggatctgga ctggcagacc
     2761 ggacggcacg cccgttgttg gtcagtttgg ctttgccagt gccgtaccat tgttgaatca
     2821 ggtcaataat atcttactgt cgcgcagtgc gaatctgccg gaagacccgc gcccgaactc
     2881 agtcactcgt ggcgttatct gctggccggg cggacagtct ttgccggaag gtgacggtaa
     2941 ctgtcgccgc cgcctggcaa cctggctgct ggacgggagt cagccgccaa ctctattact
     3001 gccggagcag gaaggcatta atggcattcg tttccccatc tggctggatg aaaatggcaa
     3061 acgtgttgcc gctgattgcc cgcaagcgcg acaagaaatg ataaatgtct ggccgctacc
     3121 gctggaaccc tggctgcccg catcagaacg tcgcgctgtg cgcttaccac cggcctcgac
     3181 aagttgcccg ccatatggtc acgatgccca gctcccgctg cagctgacag gcgttcgcga
     3241 tggcgcgatt attaaacgtt taccgggcgc agcggaagcg actttgccgt tgcaatcaag
     3301 tggaggggca ggtgaacgct ggtggtttct gaatggcgaa ccgttaactg aacgcgggcg
     3361 caacgtgacc ctgcatttga cggataaagg tgattatcaa ttgctggtga tggatgacgt
     3421 ggggcaaatc gcgacagtga aatttgtcat gcaatagtct gttttgtccc tgattgttgc
     3481 taaaactcat tttattttaa aaaaatgtta ccttcatcaa tagtcaacgg ccctgttgct
     3541 cattataatc cgcgccatct cgtacgctgg tacagacaac aacagaacaa tttacagagg
     3601 taaaaatggc tattgaacgt actttttcca tcatcaaacc gaacgcggta gcaaaaaacg
     3661 tcattggtaa tatctttgcg cgctttgaag ctgcagggtt caaaattgtt ggcaccaaaa
     3721 tgctgcacct gaccgttgaa caggcacgtg gcttttatgc tgaacacgat ggaaaaccgt
     3781 tctttgatgg tctggttgaa ttcatgacct ctggcccgat cgtggtttcc gtgctggaag
     3841 gtgaaaacgc cgttcagcgt caccgcgatc tgctgggcgc gaccaatccg gcaaacgcac
     3901 tggctggtac tctgcgcgct gattacgctg acagcctgac cgaaaacggt acccacggtt
     3961 ctgattccgt cgaatctgcc gctcgcgaaa tcgcttattt ctttggcgaa ggcgaagtgt
     4021 gcccgcgcac ccgttaataa tttcgtaaat gccgcgtgca aacgtggcat ccgtgcgcca
     4081 gaatttgtac aatgcagcgc ccccggacga gcagccgctc accggggcgt ttcttttttc
     4141 aaccctccag gggccataac gtgtaataac gaggccggaa tagcatatgt ctgaacaatt
     4201 agtcacacct gaaaacgtca ccacgaaaga tggaaaaatc aacctgctgg atctcaaccg
     4261 tcagcagatg cgggagtttt ttaaagattt aggtgaaaaa cccttccgcg ccgatcaggt
     4321 gatgaagtgg atgtatcact attgctgcga caactttgat gagatgaccg acatcaacaa
     4381 agtgttgcgc ggcaaactga aagaggtggc ggaaatccgc gcaccggaag tggttgaaga
     4441 acagcgttca tccgacggca ccattaaatg ggcgatcgcc gttggcgatc agcgcgtcga
     4501 aacggtgtat atcccggaag acgaccgtgc cacgctctgc gtctcttcgc aggtggggtg
     4561 tgcgctggag tgtaaattct gttccaccgc ccagcagggc tttaaccgca acctgcgggt
     4621 atcggaaatt attggccagg tgtggcgtgc ggcgaaaatc gtcggcgcag caaaagtcac
     4681 cggtcagcgc ccaatcacta acgtagtgat gatgggcatg ggcgagccgc tgctcaacct
     4741 gaacaacgtc gttccggcga tggaaatcat gcttgatgat ttcggttttg gcctgtctaa
     4801 acgtcgcgtc acgctttcta cttccggtgt agttccggcg ctggataaac tgggcgatat
     4861 gatcgacgtt gcactggcaa tttccctgca cgcgccgaac gacgaaattc gtgacgaaat
     4921 tgtgccgatc aacaaaaagt acaatatcga aacgttcctt gctgcggtgc gccgttatct
     4981 ggagaaatcc aacgccaatc aaggccgagt cactattgaa tacgtgatgc ttgaccacgt
     5041 taacgacggc actgaacacg ctcaccaact ggcggaactg ctgaaagata cgccgtgtaa
     5101 gatcaacctg atcccgtgga acccgttccc gggtgcgccg tatggacgca gctcgaacag
     5161 ccgtatcgac cgtttctcaa aagtgctgat gagctacggt ttcaccacta ttgtgcgtaa
     5221 aacgcgtggt gatgatatcg atgctgcctg tggtcagttg gcgggcgatg ttatcgaccg
     5281 tacgaaacgt accctgcgta aacggatgca gggtgaagcc atcgacatta aagcggtctg
     5341 atagccatta cgccacggtt actttatgta ccgtggcgta atgttttatg aatgaagtat
     5401 taagttgcaa ttgtctggtc aatattaacg gtgcgttttt gctgacttta aggcagtatg
     5461 taacgatgca acagtaagtt agccttagtg aatgtgggct ttgtcacgag cacacagacg
     5521 gtcttatact gtatgataac ggttaactta acggatgttt cgcggtgtgg gtgagcatga
     5581 tggttcaccg gcatctcaat tctcatttaa acgtacctgc agcgaatgaa tactgaagcc
     5641 acgcacgacc aaaatgaagc acttactacc ggcgctcgcc tgcgtaatgc tcgcgaacaa
     5701 ctaggactta gtcagcaggc cgttgccgag cgactttgcc tgaaggtttc cacggtacgc
     5761 gacattgaag aagataaggc acccgccgat cttgcttcaa cattcctgcg cggatatatc
     5821 cgctcttatg cgcgtctggt acatattcca gaagaagaac tgctgccagg gctggaaaag
     5881 caggctccac tacgggctgc aaaagttgcg ccaatgcaga gtttttccct cggtaaacgc
     5941 cgcaaaaaac gtgatggctg gctgatgacc ttcacctggc tggtgttgtt tgtggttatc
     6001 ggcctgagcg gtgcctggtg gtggcaagac cacaaagctc agcaggaaga gatcaccact
     6061 atggccgatc aatcttcggc ggaactgagc agtaatagcg agcaggggca gagtgttccg
     6121 ttaaatacgt cgacaactac agacccggct acaacgtcca cgccgccagc gtctgtggat
     6181 actaccgcaa ccaacacgca aacacctgcc gtaactgcgc cagcaccagc tgttgatccg
     6241 caacagaatg cggttgtttc gccttcgcag gcaaatgttg ataccgccgc gaccccggca
     6301 ccaacggcag caacaacgcc agatggtgct gcgccgttgc caaccgatca ggctggcgtg
     6361 accacgccgg tggctgatcc gaatgcgctg gtgatgaact ttactgccga ttgctggctg
     6421 gaggtcactg atgctactgg taaaaaattg tttagcggta tgcagcgtaa agacggtaat
     6481 ttgaacttaa ccggccaggc accgtacaaa ctgaaaattg gtgcgccagc cgcagtacag
     6541 atccagtatc aagggaaacc tgtcgatctg agtcgtttta tcagaactaa ccaggttgcg
     6601 cgtctgaccc tcaatgccga acaatcaccg gcgcagtaac agacgggtaa cgcgggagat
     6661 ttttcatgca taaccaggct ccaattcaac gtagaaaatc aacacgtatt tacgttggga
     6721 atgtgccgat tggcgatggt gctcccatcg ccgtacagtc catgaccaat acgcgtacga
     6781 cagatgtcga agcaacggtc aatcaaatca aggcgctgga acgcgttggc gctgatatcg
     6841 tccgtgtctc cgtaccgacg atggacgcgg cagaagcgtt caaactcatc aaacagcggg
     6901 ttaacgtgcc gctggtggct gacatccact tcgactatcg cattgcgctg aaagtagcgg
     6961 aatacggcgt cgattgtctg cgtattaacc ctggcaatat cggtaatgaa gagcgtattc
     7021 gcatggtagt tgactgtgcg cgcgataaaa acattccgat ccgcattggc gttaacgccg
     7081 gatcgctgga aaaagatctg caagaaaagt atggcgaacc gacgccgcag gcgttgctgg
     7141 aatccgccat gcgccatgtt gatcatctcg atcgcctgaa cttcgatcag ttcaaagtca
     7201 gcgtgaaagc gtctgacgtc ttcctcgctg ttgagtctta tcgtttgctg gcaaaacaaa
     7261 tcgatcagcc gctgcatctg gggatcaccg aagcgggtgg cgcgcgcagc ggggcagtaa
     7321 aatccgccat tggtttaggt ctgctgctgt ctgaaggcat cggcgacacg ctgcgcgtat
     7381 cactggcggc cgatccggtc gaagagatca aagtcggttt cgatattttg aaatcgctgc
     7441 gtatccgttc gcgaggtatc aacttcatcg cctgtccgac ctgttcgcgt caggagtttg
     7501 atgttatcgg tacggttaac gcgctggagc aacgcctgga agatatcatc actccgatgg
     7561 acgtttcgat tatcggctgc gtggtgaatg gcccaggtga ggcgctggtt tctacactcg
     7621 gcgtcaccgg cggcaacaag aaaagcggcc tctatgaaga tggcgtgcgc aaagaccgtc
     7681 tggacaacaa cgatatgatc gaccagctgg aagcacgcat tcgtgcgaaa gccagtcagc
     7741 tggacgaagc gcgtcgaatt gacgttcagc aggtcgaaaa ataataacgt gatgggaagc
     7801 gcctcgcttc ccgtgtatga ttgaacccgc atggctcccc aaacattgag ggaagcgttg
     7861 agggttcatt tttatattca gaaagagaat aaacgtggca aaaaacattc aagccattcg
     7921 cggcatgaac gattacctgc ctggcgaaac ggccatctgg cagcgcattg aaggcacact
     7981 gaaaaacgtg ctcggcagct acggttacag tgaaatccgc ttgccgattg tagagcagac
     8041 cccgctattc aaacgtgcga ttggtgaagt caccgacgtg gttgaaaaag agatgtacac
     8101 ctttgaggat cgcaatggcg acagcctgac tctgcgccct gaagggacgg cgggctgtgt
     8161 acgcgccggc atcgagcatg gtcttctgta caatcaggaa cagcgtctgt ggtatatcgg
     8221 gccgatgttc cgtcacgagc gtccgcagaa agggcgttat cgtcagttcc atcagttggg
     8281 ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct gaactgatta tgctcaccgc
     8341 ccgctggtgg cgcgcgctgg gtatttccga acacgtaact cttgagctga attctatcgg
     8401 ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg gcattccttg agcagcataa
     8461 agaaaagctg gacgaagact gcaaacgccg catgtacact aacccgctgc gcgtgctgga
     8521 ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct ccggcattag gtgattatct
     8581 ggacgaggaa tctcgtgagc actttgctgg tctgtgcaaa ctgcttgaga gcgcggggat
     8641 cgcttacacc gtaaaccagc gtctggtgcg tggtctggat tactacaacc gtaccgtttt
     8701 cgagtgggtg actaacagtc tcggctccca gggcaccgtg tgtgcaggcg gtcgttatga
     8761 cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct gtcggttttg cgatgggcct
     8821 cgaacgtctt gtattgttag tacaggccgt taatccggaa tttaaagccg atcctgttgt
     8881 cgatatatac ctggtggctt caggtgctga tacacaatct gcggctatgg cattagctga
     8941 gcgtctgcgt gatgaattac cgggcgtgaa attgatgacc aaccacggcg gcggcaactt
     9001 taagaaacag tttgcccgtg ctgataaatg gggtgcccgc gttgctgtgg tgctgggtga
     9061 gtctgaagtg gctaacggca cagcagtagt gaaggatttg cgctctggtg agcaaacggc
     9121 agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta ctgggttaag gaaggagaag
     9181 gacagcgtgg aaatttacga gaacgaaaac gaccaggtag aagcggttaa acgctttttt
     9241 gctgaaaatg gcaaagcact ggctgttggg gtgattttgg gcgttggcgc actgattggc
     9301 tggcgctact ggaacagcca tcaggttgat tctgcacgct ccgcttctct tgcctatcaa
     9361 aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca tcccggcggc ggaaaaattt
     9421 gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt tggaacttgc gcagcaattt
     9481 gttgacaaaa atgaactaga gaaagctgcc gcccagttac aacaggggct ggcagacacg
     9541 agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg ctcgcgttca ggtacagctc
     9601 aagcaggctg atgccgcgct gaaaaccctt gataccatca aaggtgaagg gtgggctgcc
     9661 attgttgccg acctgcgtgg tgaagcattg ctgagcaaag gtgataagca aggtgcgcgt
     9721 agtgcatggg aagcaggcgt gaaaagcgat gttactccgg cactgagcga aatgatgcag
     9781 atgaaaatta ataatttgtc catctgagag ggacccgatg caattgcgta aattactgct
     9841 gccaggactg ctttccgtta cccttttaag cggctgttcg ctgtttaaca gcgaagaaga
     9901 tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag tttacgccga ccacggcgtg
     9961 gagcacttcc gttggtagcg gcattggcaa cttctattcc aatcttcatc cggcactggc
    10021 ggacaacgtt gtctatgcag cggaccgcgc tggtttagta aaagcgctga atgcggatga
    10081 tggcaaagaa atctggtctg tcagcctggc cgagaaagat ggctggttct ctaaagagcc
    10141 tgcattactt tctggcggtg tgaccgtgtc tggtgggcat gtctacattg gcagcgaaaa
    10201 ggcgcaggtt tacgcgctga ataccagcga tggtactgtg gcatggcaaa ctaaagtcgc
    10261 gggtgaagca ctttctcgcc cggtggtcag cgacggtctg gtgttaatcc acaccagtaa
    10321 cggtcagtta caagcgctga acgaagctga cggcgctgtc aaatggacag ttaacctcga
    10381 tatgccttcg ctctctttgc gtggcgagtc tgcgccggca acggcttttg gtgcggccgt
    10441 cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg gaacagggcc agatgatttg
    10501 gcagcagcgt atttcccagg cgaccggttc taccgaaatt gaccgtctga gcgatgttga
    10561 cacgactccc gtcgttgtta acggcgttgt tttcgcgctg gcctataatg gtaacctgac
    10621 ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc gaactgggtt cggtgaatga
    10681 tttcatcgtc gacggcaatc gcatctatct ggtcgatcaa aatgaccggg tgatggcgtt
    10741 gaccattgat ggcggcgtta cgctgtggac acaaagcgat ctgttgcatc gcctgctgac
    10801 ttctccggtg ctgtataatg gcaacctggt ggtaggtgac agcgaaggtt atctgcactg
    10861 gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa gttgatagtt ccggtttcca
    10921 gactgaaccg gttgccgctg acggcaaact gctgatccag gcaaaagacg gaaccgtgta
    10981 ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa acggctcctg gacaggggcc
    11041 gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt tgtctgatga tttataaaaa
    11101 tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg cgccctaacg taggaaaatc
    11161 cacgttattt aaccgtctaa ctcgcacccg agatgcgctg gttgcggatt tcccgggtct
    11221 gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc cgtgagttta tctgtattga
    11281 taccggcggg attgatggca cagaagacgg tgtagaaacc cgcatggcgg aacagtcgct
    11341 gctggcgatt gaagaagcgg acgtcgtact gtttatggtg gatgcgcgcg cgggcctgat
    11401 gccggcagat gaagcgattg ccaaacatct gcgctcccgt gaaaaaccga ccttcctggt
    11461 ggcaaacaaa actgacggtc tggatcccga tcaggccgtg gttgatttct actcgcttgg
    11521 tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt ggcgtattaa gtctgctgga
    11581 gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa gaggaagtcg acgaagacgc
    11641 tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa gaagaagagg aagacgactt
    11701 cgacccgcaa agtctgccga tcaaactggc gattgtgggt cgtccgaacg taggtaagtc
    11761 tacactcact aaccgtattc ttggtgaaga gcgcgttgtt gtttatgaca tgcctggtac
    11821 gacgcgtgac agtatctaca ttccaatgga acgcgatgga cgtgagtatg tgctcattga
    11881 caccgctggc gtacgtaaac gcggtaaaat caccgatgct gtagagaaat tctccgtaat
    11941 caaaacgttg caggccattg aagatgccaa cgtggtgatg ttagtgattg atgcgcgcga
    12001 aggtatttcc gatcaggatc tctcgctgct gggctttatt ctcaatagtg ggcgctcact
    12061 tgtcattgtg gtgaataagt gggatggcct gagtcaggaa gtgaaagagc aggtgaaaga
    12121 aacgctggac ttccgtctgg gctttatcga ttttgctcgt gtgcacttta tctctgcctt
    12181 gcacggcagt ggtgttggta acttgtttga atcagtacgt gaagcgtatg acagctccac
    12241 ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg atggctgttg aagatcacca
    12301 accgccgctt gtacgcggtc gtcgtgtgaa gctgaaatat gcccacgccg gtggttataa
    12361 cccgccgatt gtggtgattc acggtaatca ggtgaaagac ctgcctgatt cctacaagc
//
LOCUS       DACAPB010000136        12182 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.136, whole genome shotgun sequence.
ACCESSION   DACAPB010000136 DACAPB010000000
VERSION     DACAPB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.136"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(218..544)
                     /locus_tag="IT904_003366"
     CDS             complement(218..544)
                     /locus_tag="IT904_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAP3193105.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSADDIAKLAGQKLVARAKPGEYVQGING
                     KWMRKS"
     gene            complement(552..737)
                     /locus_tag="IT904_003367"
     CDS             complement(552..737)
                     /locus_tag="IT904_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAP3193106.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(734..3373)
                     /locus_tag="IT904_003368"
     CDS             complement(734..3373)
                     /locus_tag="IT904_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAP3193107.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQTILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGGDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3581..4570
                     /gene="ldhA"
                     /locus_tag="IT904_003369"
     CDS             3581..4570
                     /gene="ldhA"
                     /locus_tag="IT904_003369"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP3193108.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYITLRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4681..5103
                     /gene="hslJ"
                     /locus_tag="IT904_003370"
     CDS             4681..5103
                     /gene="hslJ"
                     /locus_tag="IT904_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAP3193109.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5100..5366)
                     /locus_tag="IT904_003371"
     CDS             complement(5100..5366)
                     /locus_tag="IT904_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAP3193110.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5640..9164
                     /gene="nifJ"
                     /locus_tag="IT904_003372"
     CDS             5640..9164
                     /gene="nifJ"
                     /locus_tag="IT904_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP3193111.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVIDELLTRGENVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9530..10663
                     /gene="ompN"
                     /locus_tag="IT904_003373"
     CDS             9530..10663
                     /gene="ompN"
                     /locus_tag="IT904_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAP3193112.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10804..11238
                     /gene="uspF"
                     /locus_tag="IT904_003374"
     CDS             10804..11238
                     /gene="uspF"
                     /locus_tag="IT904_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAP3193113.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11824..>12182
                     /gene="sitA"
                     /locus_tag="IT904_003375"
     CDS             11824..>12182
                     /gene="sitA"
                     /locus_tag="IT904_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAP3193114.1"
                     /translation="MYSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEG"
BASE COUNT         3115 a         3087 c         2893 g         3087 t
ORIGIN      
        1 acgctttggg atgacgccag gcgagtatcg acgtaaattc cgctaactaa ttcgcgttgc
       61 atgtactgca acgccaatta gttaaaacag atatttgact acgcatattt cagggcaaaa
      121 ggcaatttga caggagtttt tgaatattca ccttgtttcc ggaaaataga ttaatcgcct
      181 tctcctgtag cgttatcccg atattgcggt tttgctatta agattttcgc atccatttac
      241 cgtttatccc ttgtacatat tcccccggtt tggcccgagc tacgagcttt tgcccggcga
      301 gtttcgcgat atcatctgca gacacattat tttgctttgc cagttgttgg taactcgctt
      361 tacgttcggc attaatgtcg gttactaatt tctctgtttc agcatccgtt ttcagcgcaa
      421 ccaaataacc ataaaatgtt tcacctactc gcccctgagt tctggcttca tccagagtca
      481 atgccattac attgctgctt accagcccaa caagaaacgc acaaagaagt aatgttttct
      541 tcatcatcgc ctcagaaaag atcgctacga gtttcaagca gctcttcgac atctttgtct
      601 gccttgatga tgatctcatg ctcaatttta acgttcatat tgatagtgat cggttcctta
      661 ggtgcagcga cttcaatgcg aggcgtacag ccaaccagca taaaagatga cgtcaacgca
      721 gccagtaaaa ttttcattgt ttttcctcac actcttttcc ttgcggacag tcatttcccg
      781 gcagacgtgc gttctgctcc aaccatgcct ggagattatc gccaaagcgt aaactgcgcc
      841 acagcgtaaa aatattctct tcatgatggt aattaaggtt taccgtaccg cttttaccat
      901 caacgcgact ggtacctgta atgttggcct gcatggttaa taaaccgaga ttatctaaat
      961 taatatttgt cgatgaacgg ctaatttcca tatagcgcaa ccagttaatt gctgaacccg
     1021 cagtcatatt gtctttcacc accgcatctg cggtgtcttt atccagccgc aaggtcattg
     1081 gcccgctgtt tgccagccaa ccatctttca ctatccattt ttcgttgttt agccataacg
     1141 gcaatgcacc actaaatgcc ccggacatgg cgaattgttt cggattgacg gcgctcacca
     1201 gttcgctgga tgagagatta ttcagccgca acagcgccgg gtcatgctgc ggcatacgta
     1261 attgtttcat cagtacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaagg
     1321 gttcttcttc agtccacgga taacgccctt gcaaatcagc cgtaatattt ttcgctgtca
     1381 ccagattaat cacttcggca attcgcaacg taacggggcc gcgagtcccc agatgccagg
     1441 ctccatcggc aaaacggaaa ggcaggacaa aatcgacacc gttcacctga ttatctggca
     1501 tccaggcact accgcctttc aacacgccgt gccctcccgc gcggaatcct tgttcaggcg
     1561 cagcagaaaa tgcaacctga gcatacagtt caccatcgcg taagttcatc ttccagtcgg
     1621 gtggcaccag cggctggaat acagtcagtg attgtttagg ccaccaggcg ttaccgcgca
     1681 gacgaatacc gtcccagcga ccgtttaccc gaaccgggcc aatctcacca gcatgtaaat
     1741 cgcctttaaa gaggaaatag gtcggatcgc ccccatcgac gctaaatttt aaggttgatg
     1801 gcggtaacac actgccgcca gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc
     1861 tgaaggaggg atgctgtgcg tcacgtaccc agacgacggg cttgtcgaga attaatcgcg
     1921 gcttttctac cgtcatcgta ccgtattgta actggtcgaa accggtggag agatcggtca
     1981 gcgtaatcgt gctgtcatgc cactcacctt ttcctgcgac atcccaggtg gcattcatcg
     2041 gtgtaaaact cccttttccc cagtagcgcc actgccagcg gccagcgtca gggagaaaat
     2101 cattcgccag cccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca
     2161 agatggtctg caaacgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc
     2221 agcggatttc atcgatgtcc agcgaatcga tgactcttcc cttcgaacgt aataacgcgc
     2281 ctggctcaaa ggccagcgtt gggtcagtca gacttccact tagctgcgcg ggtaagcggg
     2341 catataaaat gaggtccgcc tgtttcgctt caccggttag ctgcagaggc agctggctgt
     2401 tatccatgct taattttcct gggccaaaat ttagcaccgc gttgccttta cccgcttgcc
     2461 cctgggtcag cacactcagt cgtccgctga tcagagcgtt ctcaagccct gcctgccagt
     2521 tgtcgacttt gacacccagt cggccactca aaggaaaacc tgcatacggc caactccagc
     2581 gaccatcgct tacggtcaat tgttgacgag taatttgcca cggcaaatcg agcaacggat
     2641 cgccgttatc ccgtgccagc acaatcaatt gcccgctatt ttcctgccag tccagctctg
     2701 catccaccag tgacggttcc tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag
     2761 gaagtccatc cggcacgagc ggcatagtaa attcccccac cagtttaacc ggcggctgat
     2821 tttcaaacgc gacgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt
     2881 gaaatttaac tttttcgccc tgataacgca gttgctggat atcagaggtt aatgcgagag
     2941 agagttttcc ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg
     3001 tgttaggcag catggactgc cactgcgcga gggtttttgg agcagctggc gattgctccg
     3061 tctgcggcaa tttcgccaga caagcagaat caagttctac catgccgacg ttcaataacc
     3121 agcggctggg atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca
     3181 gataacggag atcgggaatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg
     3241 caatacgtgt cccgagcggt agccagatgc ccgccagcgt gggaacccac agcccgagcg
     3301 tcatcagcag cgtcaacggc acaagaataa tcagtaataa cagcgcgaga acggctttat
     3361 atttacccag catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg
     3421 gtattgtggc atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg
     3481 atgaattttt caatatcgcc atagctttca attatatttg aaattttgta aaatattttt
     3541 agtagcttaa atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag
     3601 cacaaaacag tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct
     3661 ggaatttttt gactttctgc tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc
     3721 ggtatgtatt ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca
     3781 cggcgttaaa tatatcaccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc
     3841 aaaagaactg gggctgaaag tagtccgtgt tccagcctat gatccagagg ccgttgctga
     3901 acacgccatc ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac
     3961 ccgtgacgct aacttctctc tggaaggtct gaccggcttt actatgtatg gcaaaacggc
     4021 aggcgttatc ggtaccggta aaatcggtgt ggcgatgctg cgcattctga aaggttttgg
     4081 tatgcgtctg ctggcgttcg atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga
     4141 gtatgtcgat ctaccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct
     4201 gacaccggaa aactaccatc tgttgaacga agccgccttc gatcagatga aaaatggcgt
     4261 gatgatcgtc aataccagtc gcggtgcatt gattgattct caggcagcaa ttgaagcgct
     4321 gaaaaatcag aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt
     4381 ctttgaagat aaatccaacg acgtgatcca ggatgacgta ttccgtcgcc tgtctgcctg
     4441 ccacaacgtg ctgtttaccg ggcaccaggc attcctgaca gcagaagctc tgaccagtat
     4501 ttctcagact acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga
     4561 actggtttaa tcttgccgct cccctgcaac ccaggggagc tgattcagat aatccccaat
     4621 gacctttcat tctctattct taaaatagtc ctgagtcaga aactgtaatt gagaaccaca
     4681 atgaagaaag tagccgcgtt tgttgcgcta agcctgctga tggcgggatg tgtaagtaat
     4741 gacaaaattg ctgttacgcc agaacagcta cagcatcatc gctttgtgct ggaaagcgta
     4801 aacggtaagc ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg
     4861 atgatttccg gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa
     4921 ctgacagcca aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa
     4981 ctcgataaca ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac
     5041 cagttaacgc tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat
     5101 taatagctgc cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt
     5161 aatgcacaca tcccaatcgc cgtaccatcc agttgacggg caacagaaag cgaaccgccg
     5221 atcattgcac aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg
     5281 tgcgccgcag tcgcctgctg aacaggctca ctactacacg ccgacaacaa taaagcggca
     5341 caccctaccc aaaacgctgc tcgcatctct tcttcctctg atcttcaagc caaacgacac
     5401 cgccataaat aataggcagc acagagggcg acgtcgagag ctgtcctgcg cgttgccccg
     5461 catttttact tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc
     5521 tgatgtgggg gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaaagat
     5581 tactactggg cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata
     5641 tgattactat tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta
     5701 tcgccatcta ccctattacc cccagttcca cgatggcaga acaggctgat gcctgggccg
     5761 gaaacggctt gaagaacgtt tggggagaca caccacgcgt ggttgaaatg cagtcggaag
     5821 cgggtgctat cgctaccgtg catggcgctt tgcagacggg tgccctttca acatcgttta
     5881 cgtcatcgca gggtttgctg ctgatgatcc cgacgctgta caaactggca ggcgaactca
     5941 caccgtttgt cctgcatgta gcggcacgta ccgttgccac acatgcactc tctatttttg
     6001 gcgatcattc cgacgtcatg gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcaa
     6061 acgtccagga agcgcaagac tttgctctca tttcgcacat cgcgacgctg aaaagccgcg
     6121 tgccatttat tcatttcttt gatggtttcc gcacgtccca cgaaatcaat aaaattgtcc
     6181 cgctggccga tgacactatt cttgatctca tgccgcaggc cgaaattgat gctcatcgcg
     6241 cccgggcgct caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt
     6301 atttccagtc tcgcgaagcc accaacccct ggtacaacgc ggtctatgac catgttgaac
     6361 aggcgatgaa tgatttcgct gccgcgacag gtcgtcagta tcagccgttt gagtattacg
     6421 ggcatccgca agcggaacgg gtgattatcc tgatgggctc tgccattggc acctgtgaag
     6481 aagtgattga tgaattgcta acccgtggcg aaaatgtcgg cgtgctgaaa gttcgcctgt
     6541 accgcccctt ctccgctaaa catttactgc aagctctgcc gggatccgta cgcagcgtgg
     6601 cggtactgga cagaaccaaa gaacccggtg cccaggcaga accgctctat ctggatgtga
     6661 tgaccgcact ggcagaagcc tttaataatg gcgagcgcga aactctgccc cgtgtcattg
     6721 gtgggcgcta tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg
     6781 ccgagctcaa cgcggctaaa ccgaaagcgc gctttacggt tggtatttac gatgatgtga
     6841 ctaatctgtc actgccgttg ccggaaaaca ccctgccaaa ctcggcgaaa ctggaagcct
     6901 tgttttatgg ccttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta
     6961 tcggtaattc cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg
     7021 gcggcctgac ggtttctcac cttcgagtga gcgaacagcc gattcgttcc gcttatctca
     7081 tttcccaggc tgattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg
     7141 ctgagcgttt aaaacctggc ggcattttcc tgctcaacac accgtacagc gcagatgaag
     7201 tgtggtcgcg cttgccgcaa gaagttcagg ccgtgttaaa ccagaaaaaa gcgcgcttct
     7261 atgtgattaa cgcggcgaaa atcgcccgcg aatgtggcct ggcggcccgt attaataccg
     7321 tcatgcagat ggcttttttc catctgacgc aaattctgcc tggcgatagc gccctcgcag
     7381 aattgcaggg tgcgattgcc aaaagttaca gtagcaaagg ccaggatctg gtggaacgca
     7441 actggcaggc tctggcgctg gcgcgtgaat ccgtagaaga agttccgttg caaccggtaa
     7501 atccgcacag cgccaatcga ccgccagtgg tttccgatgc cgcccctgat ttcgtgaaaa
     7561 ccgtaaccgc tgcgatgctc gccgggcttg gtgacgccct ccccgtttcg gcgctgccgc
     7621 cagacggcac ctggccgatg ggcactacgc gctgggaaaa acgcaatatc gccgaagaga
     7681 tccccatctg gaaagaggaa ctctgtaccc aatgtaacca ctgcgttgcc gcttgcccac
     7741 actcagctat tcgcgcaaaa gtggtgccgc ctgaagcgat ggaaaacgcc cctgccagcc
     7801 tgcattcgct ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg
     7861 caccggaaga ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc
     7921 agaatccaga gattaaagcc atcaatatga tgtctcgcct ggaacatgtc gaagaagaga
     7981 aaatcaatta cgatttcttc ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta
     8041 ttgatattcg tacatcgcag ctgattacac cgctgtttga atattcaggt gcttgctccg
     8101 gttgtggcga gacgccgtat attaaattac tgactcagct ctatggcgac cggatgttga
     8161 tcgctaacgc cactggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata
     8221 ccaccgatgc caacggtcgt gggccggcat gggcgaactc tctatttgaa gataatgccg
     8281 aatttggcct tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc
     8341 tggatcaatt tgccgataaa atcccggcgg aattactgac ggcgttgaaa tcagacgcca
     8401 cgccagaggt tcgtcgtgaa caggttgcag ctttacgcca gcaactcaac gatgttgccg
     8461 aagcacatga actgctacgt gatgcagatg cactggtgga aaaatcaatc tggctgattg
     8521 gtggtgatgg ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga
     8581 cggaaaacgt caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg
     8641 cgtcgaaagc gacaccgctg ggtgcagtaa ctaaatttgg cgagcacggc aaacgtaaag
     8701 cgcgtaaaga tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt
     8761 ctctcggcgc gcagctgaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg
     8821 ggccatcgct gatcattgct tatagcccgt gtgaagagca tggttacgat ctggcactca
     8881 gccacgacca gatgcgccaa ctcacagcta ccggcttctg gccgctatat cgctttgatc
     8941 cgcgtcgtgc cgatgaaggc aaactgccgc tggccttgga ttcacgcccg ccgtcagaag
     9001 cactggaaga aacgttactt cacgagcaac gtttccgtcg gctgaattcg cagcagccag
     9061 aagtggcaga acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc
     9121 tggcacaaat ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga
     9181 ttatccaatt ctaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga
     9241 aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata
     9301 acccgcgcag aataacgtat agcaaattaa aagtataatt tttattttgt atgtattatc
     9361 ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact
     9421 taaggcgtaa caaatgatat ttatcggcta actgaacttc tcctttagga tgttttcact
     9481 ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagcaa
     9541 agtactggca cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt
     9601 ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt
     9661 ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga
     9721 aacccaaatt aacgatcaac tcactggcta cggtcaatgg gaatacaata ttcaggcaaa
     9781 caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg ggctgaaatt
     9841 tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg
     9901 ctggaccgat atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat
     9961 gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa
    10021 tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga
    10081 aggcaccaac aacggacgtg atgttcgcca tgaaaacggt gacggctggg gtctttccac
    10141 aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac
    10201 caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg
    10261 gctaaaatac gatgctaaca atatttacct ggcaaccatg tattcagaaa cgcgtaatat
    10321 gaccccgttt ggcgacagcg attatgctgt ggcaaacaaa acccagaatt ttgaagtcac
    10381 tgcacagtac cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg
    10441 ccgtgacctg cacgctgcgg gtggtgcaga caacccggca ggtgttgatg ataaagatct
    10501 ggttaaatac gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctacgt
    10561 tgactataaa atcaacctgt tggatgaaga tgacagcttc tacgctgcca atggcatctc
    10621 taccgatgat attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt
    10681 tatggcgggc tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc
    10741 gtcattacac aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt
    10801 tttatgaaca gaacgattct tgtccctatc gatatttccg attcagaatt aactcaacgc
    10861 gtgattagcc acgttgagga agaggcaaag attgatgatg cagaggttca tttcctgacg
    10921 gtaatacctt cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg
    10981 gcaatggatg acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt
    11041 aaactgccaa ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt
    11101 ctggaattgg cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat
    11161 atcaccactt atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc
    11221 gtgctggttg tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc
    11281 gcaaatgtgc tgacattttc ccctctaatc cgtaccatac acgccacagt tttttatatc
    11341 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
    11401 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
    11461 acaagtaagg gcaaaaatca caactctctg actcataagc attttactta tttttcaatg
    11521 tgttaaatat catcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt
    11581 tttgcccctt ttgcagcgct ttgccccatt tttgccatca acaattttcc tcatcaagtc
    11641 atttcaaagc ctaataggtg ccctactttt ttcctcagta ccacgtcgcc agcaggaaac
    11701 aacctaatcc cggttcggat ctaaaattgc taatgataat cattttcatt ttgtattgct
    11761 tatgctataa aacatagcct ttgctatgtt tgttctaatt ttgctcacta taggtactaa
    11821 attatgtact caataaaaaa agtaaccatg ctcttggggt gcctcgccct cacctgctcg
    11881 atcgcatttc aggcgagtgc aactgaaaaa ttcaaggtca ttacaacatt taccatcatc
    11941 gcagatatgg ccaaaaacgt ggctggagat gctgcagaag tctcatccat aaccaagccc
    12001 ggtgcagaaa ttcatgagta tcagcctacc cctggcgata ttaaacgtgc gcagggggca
    12061 caactgattc tcgccaatgg tatgaatctg gaattgtggt tccaacgctt ttaccagcat
    12121 cttaatgggg ttccagaagt aattgtctct tcgggtgtga cgccagtagg gatcactgaa
    12181 gg
//
LOCUS       DACAPB010000137        12081 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.137, whole genome shotgun sequence.
ACCESSION   DACAPB010000137 DACAPB010000000
VERSION     DACAPB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.137"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..398)
                     /locus_tag="IT904_003376"
     CDS             complement(<1..398)
                     /locus_tag="IT904_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAP3193115.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQI"
     gene            505..2205
                     /locus_tag="IT904_003377"
     CDS             505..2205
                     /locus_tag="IT904_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAP3193116.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLVHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLSAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2270..2965
                     /gene="queC"
                     /locus_tag="IT904_003378"
     CDS             2270..2965
                     /gene="queC"
                     /locus_tag="IT904_003378"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAP3193117.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3017..3415)
                     /locus_tag="IT904_003379"
     CDS             complement(3017..3415)
                     /locus_tag="IT904_003379"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAP3193118.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(3509..3880)
                     /locus_tag="IT904_003380"
     CDS             complement(3509..3880)
                     /locus_tag="IT904_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193119.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4031..5902)
                     /gene="ppiD"
                     /locus_tag="IT904_003381"
     CDS             complement(4031..5902)
                     /gene="ppiD"
                     /locus_tag="IT904_003381"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP3193120.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQKISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6094..6366)
                     /gene="hupB"
                     /locus_tag="IT904_003382"
     CDS             complement(6094..6366)
                     /gene="hupB"
                     /locus_tag="IT904_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAP3193121.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(6575..8929)
                     /gene="lon"
                     /locus_tag="IT904_003383"
     CDS             complement(6575..8929)
                     /gene="lon"
                     /locus_tag="IT904_003383"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAP3193122.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9117..10391)
                     /gene="clpX"
                     /locus_tag="IT904_003384"
     CDS             complement(9117..10391)
                     /gene="clpX"
                     /locus_tag="IT904_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAP3193123.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(10517..11140)
                     /gene="clpP"
                     /locus_tag="IT904_003385"
     CDS             complement(10517..11140)
                     /gene="clpP"
                     /locus_tag="IT904_003385"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP3193124.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11386..>12081)
                     /gene="tig"
                     /locus_tag="IT904_003386"
     CDS             complement(11386..>12081)
                     /gene="tig"
                     /locus_tag="IT904_003386"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP3193125.1"
                     /translation="IKGHKAGEEFTIDVTFPEEYHAENLKGKAAKFAINLKKVEEREL
                     PELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVP
                     AALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELK
                     ADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAKVTE
                     KETTFNELMNQQA"
BASE COUNT         2761 a         3280 c         2892 g         3148 t
ORIGIN      
        1 attatctgat aacgaaaacc gctatagaca aatgcctgca acaacgcagg gatctctttc
       61 ccggtaaacc aaccgtcgtc attgaagata tgcatgctgg ctcgggtatc ccattgctga
      121 tacagcacca gctccgcgac atccgcaggt aaatcatcac gatgtaaaag ttcaccttcc
      181 agagaatgca cgcgcgttcc gttgccggta atcaaatatg catccagcga tagcgccccg
      241 agaatatgct gcatctccag cgcatgacgc cccgtggcaa aagtgagggt aatgtcgcgt
      301 tcacgcagtc gcgccaaagt agagagggtt ttctcaccta aatgatggtc aggcatcaat
      361 aaagtgccat ccatatcaaa tgctgccaga cgagccattt ctttctccac tctgtgacac
      421 ggttaatggt gattgagtat cacctgatat atacggaagt aatagtgaat agtgaatagt
      481 gaaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
      541 ctgtggcaac cttcagccgg aaagccgcaa accgtcaccg tcagcgaact ggccgaacgc
      601 tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc aggcacagga ggcgggatgg
      661 ctggagtggc aggcgcagtc aggacgcgga aagcgcggac aattacgctt tctggtcacg
      721 ccggaatcgc tacgcaatgc gatgatggaa caggcactgg aaaccggaaa gcagcaagat
      781 gtgctggagc tggcgcaact ggccccaggt gagctgcgca ctctgttaca gccgtttatg
      841 ggcggacaat ggcaaaacga tacacccacg ttgcgtattc cctactatcg cccgctcgaa
      901 ccgctacaac caggcttttt gcccggccgt gccgagcagc atctcgccgg gcagatattt
      961 tccggcctga cccgcttcga taataatact cagcgcccga ttggcgattt agcgcatcac
     1021 tgggaaacct ctactgacgg gttacgctgg gacttttatc ttcgttcaac cctacactgg
     1081 cataacggcg atgcagtaaa agcctcacac ttacaccagc gattattgat gctgttacaa
     1141 ctgccagcac tggatcaatt atttattagc gtgaagcgta ttgaagtcac ccatccgcag
     1201 tgtctgacct tctttttaca tcgccccgat tactggcttg cgcaccggct ggcgagctat
     1261 tgcagccatc tggtgcatcc gcaattccca ctgatcggca ctggtccttt tcgcttaaca
     1321 caattcacag cggagctggt gcgcctggag agccatgatt attaccattt gcgtcatccg
     1381 ctgcttaaag cggttgagta ctggataact ccgccgcttt tcgaaaaaga tttgggaacc
     1441 agttgtcggc atcccgtgca aatcaccatc ggcaaaccgg aggagctgca acgggtcagc
     1501 caggtcagta gtggcatcag tttaggtttt tgctatttaa cgttgcgcaa aagtccccga
     1561 ctctccctct ggcaggcgcg aaaagtgatc tccattattc atcaatccgg tttattacaa
     1621 acgttagaag tcggagaaaa cctgatcacc gccagtcatg cattactgcc aggctggact
     1681 attccgcact ggcaagtacc ggatgaagtc aaactaccga aaaccttgac gctggtttat
     1741 cacctaccga tagaacttca taccatggca gaacgcctac aggcgacact gtcagcggaa
     1801 ggctgtgaac tcacaattat ttttcataac gcaaaaaact gggacgacac gaccctactg
     1861 gcacacgcag acctcatgat gggcgacaga ttaattggcg aagcaccgga atatactctg
     1921 gagcaatggc tacgttgcga tccactgtgg ccacatgttt tcgacgctcc agcatatgca
     1981 catctgcaat cgacgctgga cgcggtgcaa gtaatgcctg atgaggaaaa ccgatttaat
     2041 gccctgaaag cggtttttag ccagttaatg gcagacgcga cgctgacgcc gctgttcaac
     2101 tatcactatc gcattagtgc ccctcccggc gtgaacggtg tgcgactgac accgcgcggc
     2161 tggtttgaat ttaccgaagc ctggcttccc gcgccgtcgc aatgaatagc tggtccgggt
     2221 taatcgcagg cgttaccata acgcttttat tgttcaagca ggattatcta tgaaacgtgc
     2281 tgtcgttgtg ttcagtggag gccaggattc caccacctgt ctggtgcagg cattacaaca
     2341 atatgatgaa gtccattgcg tgacgttcga ttacggtcag cgacatcgcg cagaaatcga
     2401 cgtggcacgc gaactggcgc tgaaactggg agcacgcgcg cataaggttc ttgatgtaac
     2461 gctgctcaac gagctggcgg tcagcagcct gacacgtgac agcattccgg tgcctgatta
     2521 tgaacctgaa gccgatggca tcccgaatac gtttgtccca gggcgtaata ttttgttcct
     2581 gacgctggcg gcaatatatg cgtatcaggt aaaagcagaa gccgtaatta ccggcgtctg
     2641 tgaaacggat ttctccggct acccggattg ccgcgatgag tttgtgaaag cactaaacca
     2701 tgccgtcagt ttgggcatgg cgaaagatat tcgttttgaa acgccgctga tgtggattga
     2761 taaagcggaa acctgggcgc tggcagatta ttacggcaaa ctggatttag tccgtaacga
     2821 aacgttgacc tgctataacg gcattaaagg cgacggttgc ggtcattgtg cggcatgtaa
     2881 tttacgcgcc aacggtttga atcattatct ggccgataaa ccgacggtga tggcagcgat
     2941 gaagcagaaa accgggttga ggtaattatt ccgggtgtcg ccggatgcgg cttgagcatc
     3001 cggcaccaca aaacgtttac ttaaccatct gctccagctt ttcgcgcaat tccccttcca
     3061 gagctaatgc tttctgcgtt ttaagatcaa tacaaacaaa cgtaataagc gcatccgcta
     3121 ccacctgccc ttccggctcc agtgtaatga cctggcttaa gatgccgctt ttaccgttta
     3181 attgctgcaa ctgactggta atagttaaca ggtcacttaa taccgctggg cgacgatagt
     3241 taatattgat attgaccacg acgaaggcga tgttatgggc cgtcatccac tgaaaactgt
     3301 cgctattttc caacccatcc cagcgggctt cttcgagaaa ttcaaggtag cgggcgttgt
     3361 tgacgtgctg gtaaacgtcg agatgatatc cacgaacttt gatttgtgtt tgcatagcgc
     3421 aataacctta cgttgttgtt ataagtacag aggtcataac tggtatgacc tcttcagtct
     3481 ggcaaaaaat tgccactatg caaattaatt acagggttaa taccgccaga ttacgttcca
     3541 ccagcgaatt gcccatcccc ggcacctgct ttagatcctc caccgtttta aacggaccgt
     3601 actcttcgcg ataactgaca atcgcctgcg ctttcttcag gccaacgcca ttcatcgcgc
     3661 gggctagctc ttccgcgctg gcattattaa tgctgacccg ggtgccttct tcgtcactgg
     3721 ctttcgccgg tactgctgct ttactttgtg ctgcaggagc ttccgctttg gtttctaccg
     3781 ttgtcggttt cgccacagaa gctgccgcca gcgcgctatg agacattccg gcacaggcca
     3841 gggacagggt aatgagcagt gctttaattc cgtgtttcat actgttttct ccttgtttgt
     3901 taacagtgga atcaccttaa cggcagtgaa aacagtaaac aaatggcaat gttcagaaat
     3961 ggaaaaggcc gcgaaagcga ccttttcttg ttacagctgc aacgcatttt tttgcgaggc
     4021 ggcttcagga ttattgctgt tccagcgcat cgccaatttt gattttcgcc tctttacgca
     4081 ggttactcat cagagcttca aagacgattt gtgcgttgtt ctgggtgata ccctgcacca
     4141 tcgctttttt ctgatcttcc ggcattgaac cttgtttcac ttcatccagc gccagcagaa
     4201 ccacattacc ttgcatatcg gtcgccatac cgtagctcgg tttgtctttc gctggcagtg
     4261 gcagtgcaaa cgccgcctgg ctaatcgggt cacgaccgga acggcttaag gttttcggct
     4321 cgccaaattt cagaccggca gcctgcatag cttccgcacc tttgccggct ttcaaatcaa
     4381 ccagcagttt ctcagcatcc actttcgcct gttgttcagc tttgttgtgc tgaaccagtg
     4441 ccttaacttg ttcctgaaca tctgccaacg gtttcaccgc ttccggtttg tgctcgctga
     4501 tgcgcagcac gaatgcgcgg tcgccgtcta cggtgatgat gtcagagttg atgcccggcg
     4561 cgccgttttc acctaccaga ccgccgttaa agatagcgtc ggcaaccggc ttgaagttca
     4621 actcttccgg caggttatct ttgctgaacc aacccgtctg agtggcttta acgccggcag
     4681 cttgctctgc accggccaga gactcggtgt cgttgcttgc cgcatcgctc actttctgct
     4741 gcagcgcgta gtacgcatcg agggcttttt cgtgtttcac tttcgccgca atgtcgtcac
     4801 gtacttcgtc taacgatttc actttcgctg gctgaatgtc gtccagacgt acaatcagga
     4861 aaccgaccga agatttgatg acaccagaca gttggccttt ttctttcaga ccagcatttt
     4921 tcagttcgtc cgggatagtg gcatcttcta accaacccat atcgccgccg ttacgagcag
     4981 agataatatc ggcagatttt tctttggcta atgcagcaaa atcaccgcct ttattcagct
     5041 catcaagtac cgctttcgct tcatcttcag ttttggtctg gatgatgctg tagcgggtac
     5101 gctgcggctg ggtgaattga tcctgatgct ggtcgtagta gctctggata tccgcatcgc
     5161 taaccggttg ctgcatcgtt gcggcatcca gcttgatgta actcacgcgg aattgttccg
     5221 gcgtcatgaa attgtttttg ttttgttcgt agtagctggc aatctcctgt tcggtcacag
     5281 gctgcttcgc cgccagcgcg ttaacatcga tagtcgcctc acgcaccacg cgttgttgcg
     5341 cgaccagtgc cgccagctcg tcggtttcac ctttcagcat aaaatcggta ccggcaacgc
     5401 cgttaatcag ctgttgggta gtgagctggt tacgcagcgc ctgggcgtac tgatcggcgg
     5461 tcatccccat ctggttgagg ataccgttat agcggctgtt atcaaatttg ccgtcaacct
     5521 ggaaggctgg ggtcgcgaaa atcgcctgtt taacctgctc atcgctgata cccagtttca
     5581 gctcacgtgc gtactgatcc agcagcgcct cgtcgatcag acgattcagc acctgttgac
     5641 gcagggtttt catatagcct tcgttcgctg ccagctcgga gtattgatcg cccagctgtt
     5701 gctgcatgcg attacgctcg ctgttgaagg cgttctcgaa ttgcccacgg ctgattttct
     5761 ggtcattcac ttttgcggcg tagttattgc ctccgccaat caggtaacca ctcacgccgg
     5821 tcaatatgaa cgacacgata atgataccga aaataatctt gagcacgaga ctgtttgcag
     5881 ccgtgcgtaa gctgtccatc atggtgtaac aacactccgc tgtagatgac tgtgtacctc
     5941 acgcaacgta tcgatgcgcg caaaggccta ttgtgacaag aaacgggggc aattgtcagc
     6001 ccacaagacg cagaaagtca ccgaatacaa ataaaaaagg cacatcagta gatgcgccct
     6061 tgaacttcgt cacatcccca ctggggacaa cccttagttt accgcgtctt tcagtgcttt
     6121 acctgcacgg aagctcggta ctttagcagc agcgatggtg atctctttac cggtctgcgg
     6181 gttgcggcca gtacgggcag cacgctcttt aacggcaaaa gtaccaaaac ctaccagtgc
     6241 tacatcatcc ccttctttca gagattcagt tacggaagca ataatagcat ctaacgcacg
     6301 gccagccgca gctttagaga tatcagcccc tgcagcaatc ttgtcgatca attgagattt
     6361 attcactctt ctcttcctct ttataattta tatcgcactt gaatccttca aggtacgaac
     6421 gcgcagcagt tatatcaggc cagccatccc cttacaacac cccttaataa gggcaagccc
     6481 gatccgccat ctaacttagc gagacaaaaa aaggctggca agcccgaatt agcctgccag
     6541 ccctgttttt attagtgcat tttgcgcgag gtcactattt tgcagtcaca acctgcatgc
     6601 cagacggttc attttgcagc gccagagtca gaacttcctc aatgcgcttc acaggatgaa
     6661 tgtccagatc ggcaattacg ttgtcaggaa tctcttccag atcgcgttta ttttcgaacg
     6721 gaattaacac tgttttaatc ccgccgcgat gcgctgccag gagtttttct ttcaaaccac
     6781 cgatcggcag tacctgacca cgcagagtga tctcaccggt cattgccaca tcggcacgaa
     6841 ccgggttacc ggtcaggcaa gaaaccagcg cggtgcacat agcaataccg gcactcggac
     6901 catctttcgg cgtcgcacct tccggtacgt ggacgtggat gtcgcgtttt tcgtaaaaat
     6961 cagggttgat ccccagtttt tccgcacgcg cacgaaccac cgttaacgcc gcctgaatgg
     7021 actcctgcat cacttcgccg agcgaaccgg tataggtcag tttgccttta cccggaacac
     7081 atgcggtttc aatggtcagc aagtcaccgc ccacttccgt ccacgccaga ccggttacct
     7141 gaccgacacg gttttcgtta tccgcgcgac catagtcgaa acgctgaaca ccgagatagt
     7201 catgcaggtt atcgccgtta atttcgatat gttttaatga cttatcgagc agtaactgct
     7261 taaccgcttt gcgacacagt ttggagattt cacgctccag accacgcacg cccgcctcac
     7321 gggtgtagta acgaataatg ccgataatgg cgctatcgtc gacggtcagc tcaccttttt
     7381 tcagtgcatt acgttcaatc tgcttcggca gcaggtgacg tttggcgatg ttcagttttt
     7441 catcttcggt ataaccggag aggcgaatca cttccatacg atccagcagc ggtgccggaa
     7501 tgttcatgga gttcgacgtc gcgacaaaca tcacgtcgct gagatcgtaa tccacttcca
     7561 ggtagtggtc gctgaacgct acgttctgct ctggatccag cacttcaagc agtgcagagg
     7621 ccggatcgcc acgcatgtca gaagacattt tgtcgatctc atcgagcagg aacagcgggt
     7681 ttttcacgcc cactttcgcc attttctgga tcagtttacc cggcatagaa ccgatgtaag
     7741 tacggcggtg accacggatt tccgcttcat cacgcacgcc gcccagcgcc atacggacat
     7801 atttacgccc ggtggctttg gcaatggact gaccaagaga ggttttacct acccccggcg
     7861 gccctaccag gcagaggatc ggtcccttga ttttgttgac acggctttga accgcaagat
     7921 actcaaggat tcgatctttc acgcgctcca gaccataatg gtcggtatca aggatttcct
     7981 gcgcctgacg caggtctttt ttgaccttgc tacgcgcatt ccacggcacc tgtaccatcc
     8041 agtcgatata accacgcact acggtcgctt ctgccgacat cggagacatc attttcagct
     8101 tctgcaactc tgcttccgct ttctcttttg cttctttcgg catcttcgcc gcgtcgattt
     8161 tgcgcttcag ggcttcgttt tcgtccggcg cgtcgtccat ttcaccgagt tctttctgaa
     8221 tagctttcat ttgctcgttc agatagtact cacgctggga tttctccatc tgctttttaa
     8281 cgcggttgcg aatgcgtttc tcaacctgca gcagatcgat ttccgattcc atcattgcca
     8341 tcagatattc cagacgttcg ttaacgtcgg acatctccag aacggactgt ttgtcagcca
     8401 gtttcagcgg catatgtgca gcaatggtat ccgccagacg cgccggatcg tcgatgctat
     8461 tcaacgacgt cagcacttct ggtgggattt ttttgttcag cttgatgtag ccttcgaact
     8521 ggctgattgc agtacgcacc agcacttcct gttctcgctc atcaatggtc ggcgactcca
     8581 gatactccgc cttcgcagaa aagtgttcgc cattgtcaga gagcgcagaa atacgcgcgc
     8641 gctgtaaccc ctcgaccagc actttgacgg tgccgtcagg cagcttcagc atctgcaata
     8701 tagaggccac ggtcccgacg gtgaaaagat cgtttacacc cggctcatcc gttgaagctt
     8761 ctttctgcgc gaccagcata atttttttat catggtccat cgccgcttcc agacaacgga
     8821 tagatttttc ccgcccgaca aataagggga tgaccatgtg cggataaacc accacatcgc
     8881 gcagcggcaa tacggggatt tcaatgcgtt cagaacgctc aggattcata gagctctctc
     8941 ttagtttaat ttccgccagg taatcagatg acacgactgt gcttcacgcc atctattaac
     9001 atgtacgtca gtatatgggg atgtttcccc cacattcaac gccgagaata gaggaaaaat
     9061 taaaggggag ataaaatccc ccctttttgg ttaactaatt gtatgggaat ggttaattat
     9121 tcaccagatg cctgttgcgc ttccggcttg ccataaatca gcaacggttt gctttgacca
     9181 tcaattaccg actcgtcgat aaccactttt tcgacgtctt ccatggacgg cagatcgtac
     9241 atggtatcga gcagtgcggc ttctacgatg gaacgcaggc cacgggcacc ggttttacgc
     9301 gccatcgctt tcttagcgat agcatccagc gcctcgtcac ggaattccag atccacgcct
     9361 tccagattaa acagcgcctg atactgcttg gtcagggcgt ttttcggctc tttgaggatc
     9421 tgaatcagag cttcttcgct cagttcattc aacgttgcga caaccggcag acgaccaatg
     9481 aactcaggga tcagaccaaa cttgatcaga tcttccggtt caacctgcgc cagcagctcg
     9541 ccttcgcttg ctttgtcgga cttcgctttt accgtcgcgc caaaaccaat tccggagccg
     9601 gtttctacac gatgggaaat cactttatcc agaccggcaa acgcaccgcc acaaataaac
     9661 aggatcttag aggtatcaac ctgcaagaat tcctgctgcg gatgtttacg cccaccttgc
     9721 ggtggaacag cagctaccgt accttcgatc agtttcaaca gtgcctgctg tacgccttca
     9781 ccggaaacgt ctcgggtaat ggacgggttg tctgacttac gagaaatctt gtcgatttca
     9841 tcgatgtaga caataccacg ctgtgctttc tggacatcgt agtcgcattt ctgcaacagc
     9901 ttctgaatga tgttttcaac gtcttcaccc acataaccgg cttcggtcag tgtagtcgcg
     9961 tcggccatgg tgaacggaac atccagcagg cgcgccagcg tttcagccag cagcgtttta
    10021 ccggaaccgg tcggaccgat cagcagaatg ttacttttgc ccaactcgac gccattgctg
    10081 gtatcgccgt tgcgcagacg tttgtaatgg ttgtataccg cgaccgccag cacttttttc
    10141 gcctgttcct ggccgataac gtaatcgtcc aggtggttgc gaatttcatg cggcgtcggt
    10201 agcgcactgc gttcacgatg cggtgcaact tctttaatct cttcgcgaat gatgtcgtta
    10261 cataaatcaa cacattcgtc gcagatatac acggatggac cggcaatcag cttgcgcact
    10321 tcatgctggc ttttgccgca aaaagagcaa tacagcaatt tgcctgagcc atctttgcgt
    10381 ttatctgtca tgggtcaaaa cctcttcttt gttctttgtg ccgcacacga cgacgcaaat
    10441 gccattctca ggcgcaagcc gcttacagcg ttgtgccgcc ctggataagt atagcggcac
    10501 agttgcgcct ctggcatcaa ttacgatggg tcagaatcga atcgaccaga ccgtattcca
    10561 ccgcttcagg ggcggaaagg aagcgatcgc gctcggtatc acgttcaatc tgttctaatg
    10621 attgacccgt atgaagcgcc ataagttcat tcatgcgccc tttaactttc agaatttcac
    10681 gggcatgaat ttcgatatcg gtcgcctggc cctggtagcc gcccaacggt tggtgaatca
    10741 tcacgcgcga attcggcagg caaaaacgtt taccttttgc ccctgcggtc agcaagaaag
    10801 cgcccatcga ggccgcctgg cccatacaga tggtgctgac atcaggcttg ataaactgca
    10861 tggtgtcata gatagacatc ccggcagtga tcaccccgcc tggggagtta atgtacagat
    10921 agatatcttt ttctgggttt tccgcttcca ggaacagcat ctgcgccaca atcaggttag
    10981 ccatgtggtc ttcaacctgg ccagtcagaa aaatgacgcg ttccttaagt agacgagaat
    11041 agatatcaaa agagcgctca ccgcgtgagg tctgttcaat gaccatcggc accagcgcca
    11101 tatggggtgc aaagttatct cgttcgccgc tgtatgacat ttccgtctcc tggataaaat
    11161 tgaaaaaacc tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt
    11221 tatgggtacg gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg
    11281 aaagaggctt ttcgcacggt tccatgcaaa attcatgaca aaaaaaagcc caccacctgg
    11341 cggtgacggg cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt
    11401 catcagctcg ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc
    11461 aacagcctgt tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct
    11521 gtagaactcg ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc
    11581 tttcacgcgc tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag
    11641 gccaacaact acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc
    11701 ttgtttttcg ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct
    11761 gtcgatcagc gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg
    11821 agacttaacg cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc
    11881 agcgcgcaga ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt
    11941 cagttccggc agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt
    12001 acctttcagg ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc
    12061 gccagctttg tggcctttga t
//
LOCUS       DACAPB010000138        12035 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.138, whole genome shotgun sequence.
ACCESSION   DACAPB010000138 DACAPB010000000
VERSION     DACAPB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.138"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            256..1488
                     /gene="mdtM"
                     /locus_tag="IT904_003387"
     CDS             256..1488
                     /gene="mdtM"
                     /locus_tag="IT904_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HAP3193126.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRVGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNSGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVLQHQAAELAEER"
     gene            1529..2809
                     /locus_tag="IT904_003388"
     CDS             1529..2809
                     /locus_tag="IT904_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HAP3193127.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIEKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRIAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            2925..4076
                     /locus_tag="IT904_003389"
     CDS             2925..4076
                     /locus_tag="IT904_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HAP3193128.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QELPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEILRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPHPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQTLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVEQLSTRVAAFIEML"
     gene            4086..4853
                     /locus_tag="IT904_003390"
     CDS             4086..4853
                     /locus_tag="IT904_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193129.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            4850..5107
                     /locus_tag="IT904_003391"
     CDS             4850..5107
                     /locus_tag="IT904_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAP3193130.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            5172..6032
                     /locus_tag="IT904_003392"
     CDS             5172..6032
                     /locus_tag="IT904_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HAP3193131.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            6100..7278
                     /locus_tag="IT904_003393"
     CDS             6100..7278
                     /locus_tag="IT904_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3193132.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWWALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7291..7845)
                     /gene="kptA"
                     /locus_tag="IT904_003394"
     CDS             complement(7291..7845)
                     /gene="kptA"
                     /locus_tag="IT904_003394"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAP3193133.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8094..8777
                     /locus_tag="IT904_003395"
     CDS             8094..8777
                     /locus_tag="IT904_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193134.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8774..9235
                     /locus_tag="IT904_003396"
     CDS             8774..9235
                     /locus_tag="IT904_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193135.1"
                     /translation="MTTQVRKNIMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9248..10420
                     /gene="iadA"
                     /locus_tag="IT904_003397"
     CDS             9248..10420
                     /gene="iadA"
                     /locus_tag="IT904_003397"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP3193136.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGTYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10485..11396
                     /gene="hypT"
                     /locus_tag="IT904_003398"
                     /gene_synonym="qseD"
     CDS             10485..11396
                     /gene="hypT"
                     /locus_tag="IT904_003398"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAP3193137.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11389..11781)
                     /gene="iraD"
                     /locus_tag="IT904_003399"
     CDS             complement(11389..11781)
                     /gene="iraD"
                     /locus_tag="IT904_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAP3193138.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(11778..11861)
                     /locus_tag="IT904_003400"
     CDS             complement(11778..11861)
                     /locus_tag="IT904_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAP3193139.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2705 a         3052 c         3127 g         3151 t
ORIGIN      
        1 tttaattaca cttaaggtgt atattttcta tgcaagccat caattaaaga ggtgtaatgt
       61 gctgatgact atttgtaatc gttatacatt ctgacctgga gtcagaaggt atttctctgt
      121 ctgtgtattc gcaggcagtg tggttgatta catgaaattc agtacatttg tccttctcgc
      181 ctgcctttcg tcattaccga cggtattgaa tttcgttttc cccattgggg ttctccggac
      241 aaggagttgt ttgttatgcc acgttttttt gcccgccatg ccgccacgct gtttttcccg
      301 atggcgttga ttttgtatga ctttgctgcg tatctgtcga cggacctgat ccagccaggg
      361 atcattaatg tggtccgtga ttttaatgcc gatgtcagtc tggccccggc tgccgtcagt
      421 ctctatctcg ctggaggcat ggcattgcag tggctgctgg ggccgctttc cgacagggtt
      481 ggccgcaggc cggtgctgat taccggggcg ctaatattta cccttgcctg cgctgcgaca
      541 atgttcacaa cgtctatgac acagtttctt atcgcgcgtg caattcaggg caccagtatc
      601 tgttttattg ccaccgttgg ttatgtcacg gtgcaggagg cgttcggaca gacaaaaggg
      661 atcaagttga tggcgattat cacctccatc gtactgattg cgccgattat tggcccgctt
      721 tccggcgcag ctctgatgca ctttgtgcac tggaaagtcc tttttgccat cattgcggtt
      781 atgggtttta tctcatttgt tggcttactg ctggcgatgc cagagacggt gaagcgcggc
      841 gcggttccat ttagcgccaa aagcgtcttg cgcgattttc gtaatgtctt ttgcaatcgg
      901 ctgttcctct ttggcgcagc aaccatctct ttaagctata tccccatgat gagttgggtg
      961 gctgtctcgc cggtgatcct gatcgacgcg ggcggcttaa caacttcgca gttcgcctgg
     1021 acacaggttc cggtgttcgg cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt
     1081 aaagatccga ccgaaccgcg ctttatctgg cgtgccgtac cccttcaact ggttggcctc
     1141 gcgctgttga ttatcggcaa tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc
     1201 accagtctgt atgctttcgg gattggtttg attttcccga ccttattccg ctttacgctg
     1261 ttttccaata acttaccgaa agggaccgtc tccgcatcgc tgaatatggt gatcctgatg
     1321 gtgatgtctg tctcggtcga aatcggccgc tggctgtggt ttaacagcgg tcgcttgccg
     1381 tttcatctgt tagccgttgt ggcaggcgtt atcgtcgttt tcaccctggc cggattgctt
     1441 aatcgcgtgc tccaacatca ggctgccgag ctggcggagg agcggtgatt tttgcgcgat
     1501 cctgccgtca ggctctattc ttaacattat gaataaactc attgaactca gacgcgccaa
     1561 aatgttggcg ctctctttac tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt
     1621 gccgcccaat ttttgggtga gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc
     1681 gctggcggac tggtttgcgg tggtggcgct gtttcgccgc gtgccgattc cgatcatttc
     1741 tcgtcatacg gcgattattc cgcgtaataa agaccggatt ggcgaaaatc tcggccagtt
     1801 cgtgcaggaa aaatttctcg atacccaatc gctggtggca ttgattcgac gtcacgaacc
     1861 ggcgttgttg attggcaact ggtttagtca gccagaaaac gcccgccgcg ttggtcagca
     1921 tctgttgcag atcatgagcg gttttcttga actaaccgat gatgcgcgta ttcagcgcct
     1981 gcttaagcgc gcggtccacc gggcgattga taaagtcgat ctttctggca ccagtgcgtt
     2041 gatgctggag agtatgacta aaaacgatcg tcatcaggtg ctgctggata cgctgatcgc
     2101 acagttgatc gcccttctcc agcgcgataa atcgcgcaag tttattgccc agcaaattgt
     2161 tcgctggctg gagagcgagc atccactgaa agccaaaatt ttgcccactg aatggctggg
     2221 cgaacatagc gcggagttgg tttctgacgc ggtgaattct ttgcttgatg atatcagccg
     2281 cgatcgtgcg catcagatcc gccatgcgtt tgatcgcgct accttcgccc tgatcgagaa
     2341 actgaaaaac gatccggaaa tggcggcgcg agccgatgcc gtaaaaagct atctgaaaga
     2401 agatgaagct tttaaccgct atctcagtga attgtggggg gatttacggg aatggctgaa
     2461 agcggatatc aacagtgaag attctcgtgt gaaagaacgt atcgcgcggg ctggtcaatg
     2521 gtttggcgaa acgttaattg ccgatgatgc cttgcgggcg tcgttaaatg gtcatctgga
     2581 acaagccgca caccgcattg cgcctgagtt ttccgcattc ctgactcgcc atatcagcga
     2641 cacggtaaaa agctgggacg cacgggacat gtcgcggcaa atcgagttaa atatcggcaa
     2701 agatctccag tttatccgtg tcaacggtac gctggttggc ggttgtattg gacttatttt
     2761 gtatttgctg tcgcagctcc cggccttgtt ccccctcggc aatttataga aatccatgaa
     2821 aaactaagtg gctaaccgcg agagagatca aataaaccac actaaaagaa tggtaataat
     2881 gtggcctctt ttcattatct cccgtggtac ggggaaggaa aatcatgtca cttgtcaccg
     2941 atctacccgc cattttcgat cagttctctg aagctcgcca gaaaggcttt ctcaccgtca
     3001 tggatctcaa ggagcgcggc attccgctgg ttggcaccta ctgcaccttt atgccgcaag
     3061 agctcccgat ggcagccggt gcggttgtgg tttcgctatg ttccacctct gatgaaacca
     3121 ttgaagaagc cgagaaagat ctgccgcgca acctctgccc gctgattaaa agcagctacg
     3181 gcttcggcaa aaccgataaa tgcccctact tctacttttc tgatctggtg gtcggtgaaa
     3241 ccacctgcga cggcaaaaag aaaatgtatg aatacatggc ggagtttaag cccgttcatg
     3301 tgatgcaact gccaaacagc gttaaggacg atgcctcgcg tgcattatgg aaagccgaga
     3361 tactgcgctt gcaaaaaacg gtagaagaac gttttgggca cgagattagc gaagatgctc
     3421 tgcgcgatgc cattgcgctg aaaaaccgcg aacgtcgcgc actggcaaac ttttatcatt
     3481 tggggcagtt aaatcctcca gcgcttagcg gcagcgacat tctgaaagta gtctacggcg
     3541 caaccttccg gttcgataaa gaggcgttga tcaatgaact ggatgcaatg accgcccgcg
     3601 ttcgtcagca gtgggaagaa ggccagcgac tggacccgca cccacgtatt ttaatcaccg
     3661 gctgcccaat tggcggcgca gcagagaaag tggtgcgcgc gattgaagag aatggcggct
     3721 gggttgtcgg ttatgaaaac tgcaccgggg cgaaagccac cgagcaatgc gtggcggaaa
     3781 cgggtgatgt gtacgacgcg ctggcggata aatatctggc gattggctgc tcctgtgttt
     3841 cgccgaacga tcagcgcctg caaacgctca gccagatggt tgaagaatat caggtcgatg
     3901 gcgtagttga tgtgattttg caggcgtgcc atacctacgc ggtggaatcg ctggcgatta
     3961 aacgtcatgt gcgccagcag cacaacattc cttatatcgc tattgaaaca gactactcca
     4021 cctcggatgt cgagcagctc agcacccgtg tcgcggcctt tattgagatg ctgtaaggag
     4081 tggcagtgac atattcgatt ggcattgatt ccggctcaac cgccaccaaa gggatcttac
     4141 tggctgacgg cgtgattacg cgccgtttcc tcgttccaac accctttcgc ccggcaacag
     4201 caattactga agcctgggaa actctgcgcg aagggttaga gacaacgccg tttctgacgc
     4261 tcaccggcta cggacggcaa ctggtggatt ttgccgataa acaagtgacg gaaatctcct
     4321 gtcacgggct gggcgcacgg tttcttgagc cagcaacgcg cgcggtaatc gacatcggtg
     4381 gtcaggacag caaagtgatt cagcttgatg atgacggtaa cctgtgcgat ttcctgatga
     4441 atgacaaatg cgcggcaggc accgggcgtt tcctggaggt gatctcgcgc acgcttggca
     4501 ccagcgtcga gcaactcgac agcattaccg aaaatgtcat accgcacgcc atcacgagta
     4561 tgtgcacagt gtttgctgaa tcagaagtga tcagcctgcg ctctgcgggc gtagcgccag
     4621 aagcgattct cgccggagtg attaacgcga tggcgcggcg gagcgccaat ttcattgctc
     4681 gtctctcctg tgaagcgccg attctgttta ctggtggcgt tagccactgt caaaggttta
     4741 cccacatgct ggaaagccat ctgggaatgc cggtacaaac tcaccctgat gcgcaattcg
     4801 ctggtgcaat tggcgcggcg gtgatcggtc agcggcagag aaaacgcgca tgaaagagta
     4861 tctgttttta tttcactcca cggtcggcgt catacaaacc cgcaaggcgt tgcaggcagc
     4921 gggcatgact tttcgcgtca gcgatattcc ccgtgattta cgcggcggct gcgggttatg
     4981 catttggctg acctgtccgc ctggcgagga aattcaatgg gtgatccctg ggcatacgga
     5041 atcagtttat tgtcagcagg atggtggctg gcgctgcatt gcacattacg ggatttctcc
     5101 gcgttaagga ttcattaaga tcgctacgta tacttctcga tatcttattg atttgcctta
     5161 aagggattac cgtgacaaaa agtatttcgt taagtaaacg catcttcgtt attgtgattc
     5221 tttttgtcat tgtggcagtg tgtactttct tcgtgcaatc ttgcgcccgt aaaagcaatc
     5281 atgcagcatc atttcagaat taccacgcca ccattgacgg caaagaaatt gcaggcataa
     5341 cgaataatat ctcttcgctg acctggtcgg cacaaagcaa tactcttttt agcactatca
     5401 acaaacctgc cgccattgtt gaaatgacca ccaatggcga tttgatccgc acaattcctc
     5461 tggattttgt taaggatctc gagacgatcg aatacattgg cgataatcag tttgtcatta
     5521 gcgatgagcg cgattatgcc atttatgtga tttcactgac cccaaactcg gaagtaaaaa
     5581 tcctcaaaaa aataaaaatc ccgctgcagg aatccccaac caattgcggt tttgaaggat
     5641 tggcctattc tcgccaggat cacactttct ggttttttaa agagaaaaac ccgattgagg
     5701 tctacaaagt taacgggctg ttaagtagca atgaattgca catcagcaaa gacaaggcac
     5761 tacaacggca atttacttta gatgatgtgt ccggcgcaga atttaaccaa caaaaaaata
     5821 cgcttctggt gctgtctcat gaatctcgcg cactccagga agtaacgctg gctggcgagg
     5881 ttataggtga aatgtcacta accaaaggca gtcgaggttt gtcgcataac atcaaacagg
     5941 ccgaaggggt tgcgatggat gcctcaggta atatttatat cgttagcgaa ccaaatcgtt
     6001 tttatcgctt tacccctcaa tcatcgcatt aacaggctgg catgtttcac ctttttgtta
     6061 ttaacttgtt aacaacaaaa atgacaaaca aggttaaaca tgccttcgcc ctcgcatccc
     6121 gtagaacgct tttctttcag caccgcgctt ttcgggatgc tggttctgac cttaggtatg
     6181 ggtttaggcc gcttccttta tacgcctatg ttgcccgtca tgatggcgga aggatcgttt
     6241 tcatttagcc agctctcgtg gattgccagc ggcaactatg cggggtatct ggctggcagt
     6301 ctgctatttt cgtttggcgc atttcaccag ccatcgcgcc tgcgcccatt tctgttggct
     6361 tctgccctgg cgagcgggtt gttgatcctc gcaatggcat ggttgctgcc atttattctg
     6421 gtgttattga ttcgcgtcct ggcaggtgtc gccagcgccg gtatgctgat ttttggttcg
     6481 acgctgatta tgcagcacac gcgccatcct tttgtgctgg cagctttgtt ttctggcgtt
     6541 ggcattggca tcgcactggg caatgaatat gttctggcag gcctgcattt tgacctctcc
     6601 tcgcaaacgt tatggcaagg cgccggggcg ctttctggca tgatgctgat tgcacttacg
     6661 cttttaatgc cctcgaaaaa acacgccatc acaccaatgc cattggcaaa aacggagcaa
     6721 cagataatga gctggtggtt actggctatt ctgtacggcc tggcgggatt tggttatatc
     6781 atcgtcgcga cttacctgcc gctcatggcg aaagatgcag gctcaccatt gttaaccgcc
     6841 catctctgga cgttagtcgg cttatcaatt gtgcctggtt gctttggctg gctatgggca
     6901 gcaaaacgtt ggtgggcttt gccctgcctg acggcgaatt tgctggtgca ggctatctgt
     6961 gtgctgctta ctctcgccag cgactcgcct ctcttgctta tcatcagcag tcttggtttt
     7021 ggcggtacct ttatgggcac gacatctctg gtgatgacta tcgcccgcca actgagcgta
     7081 ccgggaaatc tcaatctttt aggctttgtg acgctcattt acggcattgg acaaatcctc
     7141 ggcccggcgc tgaccagtat gctcggcaat ggaacatcgg cgcttgccgg cgccactctc
     7201 tgcggcgcgg cggcgttgtt tattgctgcg cttatcagca cagtacaatt atttaagctg
     7261 caagtggtca cttcatgagg agtgtttccc ttaccactca agaaattcga ccgcaaccgt
     7321 tgatgtcagc cagaccccgt tttccgcttg ccagaaaggg aggcctcgtt tcgccatttc
     7381 ctgtgctttg acggttaaaa taaccggcga tccgtgtctt gctcccactt tgcgtgccgt
     7441 tgcttcatca gcagagagat gtacgtagtg acgctcacct gcaatcagcc cctgtttttt
     7501 tatctcgtca aggaaacggc ttgccgtacc gtggtagaga aactgtggcg gtgttttttc
     7561 ggcaaaggaa atagcgacct gcgaagtcga atgcccctga accgcgcgga tgcacctacc
     7621 atcactggaa taactaaaac gctttttatc gctggtggca accacggtat ccagcaatgc
     7681 acgggttaac cgcttgcccg ctttttgggc acaaagtatg agcttatcga tatccgccca
     7741 tccttcacgg tccagtacga taccaatcgc ctccggtttg tgtcggagga caaaacttaa
     7801 aaacttgctc gtatccgcca gctctttttc gttgtacttt gccatcgcgt tttcgtccct
     7861 ggattatctg ttttttattt actatgtcat tgtcactggg tttatgaatt ttctgcaact
     7921 caatcctgcg tagaaccttt tccacacagc gtaattttac cgctatgcaa aaaacgcata
     7981 gtcagaaaca tacttgcatt ttccccatac ccacatggct gataaggttt ttcagctcca
     8041 ggaattttcc tggctcaagc aacacacaaa cacaaaaaac aatggggatt gttatgacac
     8101 aacaagggga tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt aaagggtatc
     8161 tggctttttt tctcaccata atcttttttt ccggtgtttt ttccgggaca gacagttggt
     8221 ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg gcaactgcct ggtgcgaacg
     8281 gagccaccac ctcgtttcgc ggcgcgggtg gcgcgggggc taaagatggc tttctctttg
     8341 cgctggagct ggcaccgtca gtcattctct ctctggggat tatttcaatt accgatggtc
     8401 ttggcggatt acgcgccgcg cagcagttaa tgacgccagt gctaaaaccg ttgttgggca
     8461 ttccagggat ctgctcgctg gcgctgatcg ctaacctgca aaacaccgat gccgccgcag
     8521 gtatgaccaa agagctggcg caggaaggtg aaattaccga acgcgataaa gtgattttcg
     8581 ccgcatatca aaccagcggt agcgccatta tcaccaacta cttctcttcc ggcgtcgcgg
     8641 tgtttgcctt tctgggcacg tccgtaatcg tacctttagc agtcatcctg gtgtttaaat
     8701 ttgtcggagc caacattttg cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc
     8761 aaggagcgca agcatgacaa ctcaggtccg caaaaacatc atggatatgt ttatcgatgg
     8821 tgcgcgccgt ggctttacta tcgccacgac caacctgctg ccaaacgtgg tgatggcgtt
     8881 tgtcatcatt caggcgctga aaattaccgg cctgctcgac tgggtggggc atatctgtga
     8941 acccgtaatg gcgctgtggg gattacccgg tgaagcggca accgtccttt tagccgcatt
     9001 aatgagtatg ggtggcgcag tcggtgttgc cgccagtctg gcaaccgctg gcgcattaac
     9061 cggtcacgat gtcaccgttt tactgcccgc gatgtattta atgggcaacc cggtgcaaaa
     9121 tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa tattacccgc atatcattac
     9181 ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg cagcttattg tttaacaagg
     9241 agttaccatg attgattata ccgcagccgg ttttaccctg ctgcagggag cgcatttgta
     9301 tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa ttatcgccgt
     9361 tgccagtaat atcccttctg acattgtacc ggactgcacg gttgtcgatc tcagcgggca
     9421 gatcctctgc ccaggtttta ttgatcaaca cgtccatttg attggcggtg gcggcgaagc
     9481 aggtcccacg acgcgcacgc cggaagtggc gttaagtcgt ctgacggaag caggcgtcac
     9541 gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc cacccggaat ccctgctcgc
     9601 caagacccgt gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg gcacttatca
     9661 tgtcccttcc cgcactatta cgggttccgt ggaaaaagac gtggcgatta tcgatcgcgt
     9721 gattggcgtg aaatgcgcca tctctgatca ccgttctgcc gcaccggacg tttatcacct
     9781 ggctaatatg gcggcggaat cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac
     9841 cgtgttccac atgggcgaca gcaaaaaggc gttacagcct gtctatgacc tgctggaaaa
     9901 ctgcgatgtg ccgattagca agttgctgcc gacccacgtt aaccgcaacg ttccgttgtt
     9961 tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc gatatcacca gcagcattga
    10021 cgaaccagtc gcccctgccg aaggtattgc ccgcgccgtt caggcgggta ttccgctggc
    10081 acgcgtcacc ctcagctccg acggcaacgg tagccagccg ttcttcgatg acgaagggaa
    10141 tttaacccat atcggtgttg ccggttttga aacgttgctg gaaaccgtgc aggtgctggt
    10201 caaagattat gatttcagta tcagcgatgc cctgcgcccg ctcaccagta gcgtagccgg
    10261 tttccttaac ctgagcggga aaggcgaaat tctgccaggc aatgacgcag acttactggt
    10321 catgacgcca gaattacgca ttgagcaggt atacgctcgc ggcaagctga tggtcaaaga
    10381 cggcaaagcc tgcgtgaaag gaacgtttga aacggcataa acggttgtca gccgcccgaa
    10441 tggacgtaga atgcccgcaa gggcggctga cagagtaaaa cgtaatggat gactgtggcg
    10501 cggttttgca taatattgaa actaaatggc tttatgattt tctgaccctg gaaaaatgcc
    10561 gcaatttttc ccaggcagca gtcagtcgca acgtctcgca accggcattc agccgccgca
    10621 tccgtgcgct ggaacaggcg attggcgttg aattgtttaa ccgccaggtg acgccgctgc
    10681 aactctcgga acaaggtaaa atattccatt cgcagatccg ccatctgttg caacagttag
    10741 agagcaacct tgcagaactc cgtggtggca gcgattacgc gcaacgcaaa attaaaatag
    10801 ccgctgcaca ctctctttcc ctcgggctgt taccgtccat tatcagccag atgccgccgc
    10861 tctttacctg ggcaattgaa gctattgatg tcgatgaagc ggtcgataaa ctgcgtgaag
    10921 ggcaaagtga ctgtattttt tcctttcacg acgaagattt gctggaagcg ccgtttgacc
    10981 acattcgctt atttgaatct caattgttcc ccgtttgcgc cagtgacgaa cacggagaag
    11041 cactttttaa cctcgcgcag ccacactttc cgttactgaa ttacagccgc aactcctaca
    11101 tggggcgatt gattaatcgc accctgacgc gccacagtga gttaagtttc agcacctttt
    11161 ttgtctcttc gatgagcgag cttttaaagc aggttgccct cgacggctgt gggattgcct
    11221 ggctaccgga atacgccatc caacaagaaa ttcgcagcgg gcagctcgtt gtactgaatc
    11281 gggacgaact ggtgatcccg attcaggcct atgcataccg gatgaatacc cgcatgaatc
    11341 cagttgccga acgcttctgg cgtgaactgc gcgagctgga gattgtgctt agctgacatt
    11401 ctccagcgtc gcactgcgcc aaccgccatt atcccagacc agatcaaacg ttagcgattc
    11461 atcgtcgtga tacagcatta tctcaaggct gatcgccaga gtgtggcagt acgcttcttt
    11521 caaattaacc tgcaacgccg caatccgtgg ctcatgaaat aacagtgcag aacgcagctg
    11581 acggcaatac cagctctgga gatgtttgtc gctcgggtta tgcacttgcc actcgggcat
    11641 gcctgaaaca agtaatgacg gtaatgccga atgaggagaa ttaaaaagag tgaggagatc
    11701 gctgcatact gatttacgca gcggtgacgt tttattgcct gaaatttcag gtaaaacagc
    11761 ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt tagcaacata tgcggcttgc
    11821 cctgaacacc gggcaccact gtattgatgc tcattttcca tagccattcc cttttgttca
    11881 ttacatccat gtcagtctga gacaggcctg aatcctgtca gcaaagtata aaaccttact
    11941 tgcctatgaa tatctactga cttttcagga aaaaagtcat gatttgataa aaatcatcaa
    12001 actcaaaaaa aaaccgctag tatattctga atatt
//
LOCUS       DACAPB010000139        11980 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.139, whole genome shotgun sequence.
ACCESSION   DACAPB010000139 DACAPB010000000
VERSION     DACAPB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.139"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            22..174
                     /gene="hokA"
                     /locus_tag="IT904_003401"
     CDS             22..174
                     /gene="hokA"
                     /locus_tag="IT904_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAP3193140.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(361..573)
                     /gene="cspA"
                     /locus_tag="IT904_003402"
     CDS             complement(361..573)
                     /gene="cspA"
                     /locus_tag="IT904_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAP3193141.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(854..1144)
                     /locus_tag="IT904_003403"
     CDS             complement(854..1144)
                     /locus_tag="IT904_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAP3193142.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1578..2288
                     /locus_tag="IT904_003404"
     CDS             1578..2288
                     /locus_tag="IT904_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAP3193143.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2338..3312)
                     /gene="ghrB"
                     /locus_tag="IT904_003405"
     CDS             complement(2338..3312)
                     /gene="ghrB"
                     /locus_tag="IT904_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAP3193144.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3416..4075)
                     /locus_tag="IT904_003406"
     CDS             complement(3416..4075)
                     /locus_tag="IT904_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAP3193145.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4228..6561
                     /locus_tag="IT904_003407"
     CDS             4228..6561
                     /locus_tag="IT904_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP3193146.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6530..6970)
                     /locus_tag="IT904_003408"
     CDS             complement(6530..6970)
                     /locus_tag="IT904_003408"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP3193147.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6967..7530)
                     /gene="tag"
                     /locus_tag="IT904_003409"
     CDS             complement(6967..7530)
                     /gene="tag"
                     /locus_tag="IT904_003409"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP3193148.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7688..8386
                     /locus_tag="IT904_003410"
     CDS             7688..8386
                     /locus_tag="IT904_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP3193149.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8615..9823
                     /locus_tag="IT904_003411"
     CDS             8615..9823
                     /locus_tag="IT904_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3193150.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10147..11838
                     /gene="eptB"
                     /locus_tag="IT904_003412"
     CDS             10147..11838
                     /gene="eptB"
                     /locus_tag="IT904_003412"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAP3193151.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
BASE COUNT         2814 a         2928 c         3097 g         3141 t
ORIGIN      
        1 aatcaacaca ggaggctggg aatgccgcag aaatatagat tactttcttt aatagtgatt
       61 tgtttcacgc ttttattttt cacctggatg ataagagatt cactgtgtga attgcatatt
      121 aaacaggaga gttatgagct ggcggcgttt ttagcctgca aattgaaaga gtaagagtct
      181 tcggcgggaa attattcccg ccttacttac ggcgttgcgc attctcattg cacccaaatt
      241 tattcttcac aaaaataata atagatttta ttacgcgatc gattatttat ttcctgaaaa
      301 caaataaaaa aatccccgcc aaatggcagg gatcttagat tctgtgcttt taagcagaga
      361 ttacaggctg gttacgttac cagctgccgg gcctttagcg ccgctttcga tggtgaagga
      421 cactttctga ccttcgtcca gagatttgta accatcgttc tggatagcag agaagtgtac
      481 gaacacatct ttagagccat cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa
      541 ccattttacg ataccagtca ttttaccgga catagtgtat tacctttaat aattaagtgt
      601 gcctttcggc gatatggcgt gctttacaga ttttgaagcg ttaaaggaat gtgcactacg
      661 aggggtatca acgataactc ttgaagggac ttgccttact acactgcttt aatggtctgt
      721 acgtcaaacc gttgatgtgc attaagccac gcattggcgg gtgatgcaac aattattttt
      781 catatttatg attaatcggt tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa
      841 agtggataaa agtctattcc atcaactgct tacttaatgc cgggttggct tgaatcaaac
      901 gcatcaattt tagttcggca cttgaaggct tcacgcgtct ggattcccat tcctttacca
      961 tggcgactga tacgcccaaa acccgggcga aatcatcgat ttttaatcct gtcccttttc
     1021 gtaactgctc aatttcggta caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt
     1081 catctttaaa aacaatctgt tccaggctgc tcaacagctc atgcattgga tctttatatt
     1141 ccattgaaaa ctcctcaaat cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga
     1201 atagtcggga aaagcagaca cagcggggtg aagtcagggc atgctctggc tatgattcga
     1261 ttcagcctgt catttagcgt gacttatagc cacctaacga tctgataaat gccgatatat
     1321 ctcttaccac tcctgttttg taaagatttc gaaaatctaa ccttctgcga aggcgtaaat
     1381 ctctggctac aaaaaaggca acggcactat cattttttaa caatgaaaca acaaattaac
     1441 cttgtttcat tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag
     1501 cctgtcctgg actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg
     1561 gtgaaaggag taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg
     1621 cggcgttatt aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta
     1681 aagcgtttat cgacttcctg cagaatacag tgatgcgtag cggtgaacgt ctaccaaccc
     1741 tgactgccga tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg
     1801 gttattctca gcaggtgaat caggcgatgg attccggtct gcgcccggtt gtagacagcg
     1861 ttaacgcaat tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga
     1921 acggttcgct gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg
     1981 cgcactctgc gctaaaacag agcgatgatt tgaaaccggt ctttgatcag gcattcacca
     2041 aagtggtaac gacgccagct gatgcattgc aaccgttgat tccggcggca caaactttca
     2101 cgcaacaact ggtgatggtt ggagactaca ttgctcagca aggtactcag gtgagctttg
     2161 tcgcaaatgg catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg
     2221 cgccattacc ggcacagcat caggcgttta atcaggcctg gactacagca gttactgcaa
     2281 cccaataaag agtaaaagcc cgagcgatta tcgggcttta ctctacgcag tcgcggctta
     2341 gtccgcgacg tgcggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa
     2401 attatccacg gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg
     2461 cggtactgcg acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg
     2521 ttcgaagaca tccagcccgg cagcgtgaat ttcgcctttc tgcaatgctg cgatcagtgc
     2581 attttcgtca accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt
     2641 ggcgaattgt tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca
     2701 aacgaaatct gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc
     2761 ttctgcttct ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg
     2821 cgcacgttgt gccagcgcca tgccgatccg tcccatcccg acaatgccca gtgttttatg
     2881 gtgaacgtca gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac
     2941 ccgttctgct acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc
     3001 ggcgacggtt tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag
     3061 cgcatcgaca tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg
     3121 cattttttcc agcaatgcgg catcaacatt ctcgtttgaa cccagtaaac cttcagcttc
     3181 ggcaaaaatt gctgcatttt gttcgacggt ttgtgggctg aggtttgcca cctggtgaac
     3241 ggtgaaatgc tcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac
     3301 ggacggcttc atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga
     3361 tgaaggaaaa ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca
     3421 gcgggcttaa ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc
     3481 tggcgattgg gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct
     3541 gggtgatcaa cgcgctggca acggaatccg cacgctgctg ggagagacgc atgttcaggt
     3601 cgtgaccccc cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt
     3661 tcagcaccat tgccacgccg gtcagggtgt tagcgcctgc cggtttcaga gtcgcgctgc
     3721 tgctgtcgaa ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta
     3781 cgctaacacc agtgccgcgc attttgtcgc gcagcttcgc ttcctgcaca tccatgtaat
     3841 aaccaacgcc gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc
     3901 gatctttctt cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg
     3961 cgccgatagc agatttacct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag
     4021 ataccgccag agcgccactc actacggcgg caataagata aacacgtttc ttcattgtta
     4081 atccttaata acctttttat tctttgccac gggttccgtg gcgggagatt atgccgcgtg
     4141 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4201 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4261 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4321 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4381 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4441 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4501 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4561 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4621 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggcgcg
     4681 gcacaggcga tcatgccgta tgtcgtgggt ggtagcgaag tttatcaaca gcagaccagt
     4741 tggccgctgg tgctggaaca tagcgatgtc gtggtgctgt ggagtgctaa tccactcaat
     4801 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttctgcactg
     4861 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     4921 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     4981 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5041 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5101 accgccgaat gggcagcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5161 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5221 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5281 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     5341 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtagat
     5401 aaaatccctg tggcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     5461 aacggtatgg accgacattt tccggatatt cgttttatct ggtgggcggg cggtgccaac
     5521 tttactcatc atcaggatac caaccgcctg atccgtgcct ggcaaaaacc ggagctggtg
     5581 gtgatctccg aatgtttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg
     5641 accacctcgt ttgagcgtaa tgatctcacc atgaccggcg attacagtaa tcagcatctg
     5701 gtgcccatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     5761 gccgagttaa gtgaacgctg ggagaagggc ggttatgcac gttttacgga aggaaaaagt
     5821 gagctgcaat ggctggaaac gttttataac gttgcccgac agcgcggggc aagccagcag
     5881 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     5941 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gcaggcgcat
     6001 ccattaaaaa ccgccagcgg taagattgaa atcttctcgc agcgtattgc cgattacgct
     6061 tatccggatt gcccggggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6121 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6181 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6241 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6301 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     6361 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     6421 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     6481 aatacggcgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     6541 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     6601 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     6661 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     6721 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     6781 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgca ttggcaagat
     6841 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     6901 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     6961 cccgaatcat ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag
     7021 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7081 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7141 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg
     7201 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7261 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7321 tgcggcaacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     7381 gaggacggtg atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag
     7441 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     7501 gtcctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     7561 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     7621 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     7681 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     7741 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     7801 tacgcaaccc ggactttcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     7861 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     7921 tctggcggaa gaaacccctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     7981 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8041 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8101 cagcgtgagt accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8161 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8221 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8281 gttgctcaat caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa
     8341 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     8401 ttgaaaccat taaataacaa tagttgtggc gatagtgggt gctaacttac caaataataa
     8461 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8521 tggcatttca tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga
     8581 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8641 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     8701 ctggagcctg tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc
     8761 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     8821 acaggaacgt tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg
     8881 cttcttcctg acagcgcatt ctgacaacct gatgatgctg tggttaagcg ccggtgtgct
     8941 ggtgggactg gcagatggcg cgggttatct gctgacgctc tctaactgtg tgaagtggtt
     9001 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9061 gggtttcaaa tttatcgaca cgcagctgct ggaaacggtc ggtctggaaa aaacctttgt
     9121 gatttgggga gcgattgcgc tgttgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9181 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9241 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgaccg cctgcatgag
     9301 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9361 ggtttccgca gccaatgcag tcactgttat ttccatcgcc aacctttcag gtcgtctggt
     9421 gctgggtatt ctgtctgaca aaatcgcccg tatccgtgtt attaccattg gtcaggtgat
     9481 atcgctggtg ggtatggcgg ccctgctgtt tgcaccattg aatgcagtga cgttctttgc
     9541 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc cgtcactggt
     9601 cagtgagttc tttggcctca ataacctggc gaaaaactac ggtgtgattt atctcggttt
     9661 cggtatcggt agcatttgtg gttcgattat cgcctcactg tttggcggct tctatgtgac
     9721 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
     9781 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
     9841 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
     9901 aaaatttggg ccaattaact ggcccttttt actgcttttt gtaacgctaa agtctctttt
     9961 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttccct
    10021 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10081 ttccctgccg ggcctgaaaa gccactaagc agggtgttat cacctgtttg tccagggttt
    10141 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10201 tatattggcc tttttatgaa tggcgcggtt ttttaccgcc gcttcggcag ctatgcgcac
    10261 gattttaccg tctggaaagg catttctgct gttgttgaac tggccgccac cgtactggtg
    10321 accttctttt tactacgtct tctttcgctg tttggccgcc gcagctggcg tattctggca
    10381 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    10441 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    10501 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    10561 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    10621 cgcagcctgg cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    10681 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    10741 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    10801 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaaattcact
    10861 tatcaggcac cgcaaaacgt tgatgacact tatgtcgtgt ttatcatcgg tgaaaccacg
    10921 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    10981 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11041 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11101 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11161 agcgaaatgt ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt
    11221 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11281 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    11341 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    11401 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    11461 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    11521 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    11581 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    11641 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    11701 atgaaagtgc cacgccgtca cgtagagctg tacgatacca tcatgggttg tcttggctat
    11761 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcaggcaaaa
    11821 gaggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    11881 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    11941 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
//
LOCUS       DACAPB010000140        11949 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.140, whole genome shotgun sequence.
ACCESSION   DACAPB010000140 DACAPB010000000
VERSION     DACAPB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.140"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            142..885
                     /locus_tag="IT904_003413"
     CDS             142..885
                     /locus_tag="IT904_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3193152.1"
                     /translation="MAIALVTGGSRGIERATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(942..2375)
                     /locus_tag="IT904_003414"
     CDS             complement(942..2375)
                     /locus_tag="IT904_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP3193153.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            2420..2731
                     /locus_tag="IT904_003415"
     CDS             2420..2731
                     /locus_tag="IT904_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP3193154.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            2895..3554
                     /locus_tag="IT904_003416"
     CDS             2895..3554
                     /locus_tag="IT904_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP3193155.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3750..4730)
                     /gene="ygfZ"
                     /locus_tag="IT904_003417"
     CDS             complement(3750..4730)
                     /gene="ygfZ"
                     /locus_tag="IT904_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAP3193156.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSVLPSKEKQVIREDATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4973..5239
                     /gene="sdhE"
                     /locus_tag="IT904_003418"
     CDS             4973..5239
                     /gene="sdhE"
                     /locus_tag="IT904_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAP3193157.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            5220..5627
                     /locus_tag="IT904_003419"
     CDS             5220..5627
                     /locus_tag="IT904_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193158.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5667..6188)
                     /gene="fldB"
                     /locus_tag="IT904_003420"
     CDS             complement(5667..6188)
                     /gene="fldB"
                     /locus_tag="IT904_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAP3193159.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            6300..7196
                     /gene="xerD"
                     /locus_tag="IT904_003421"
     CDS             6300..7196
                     /gene="xerD"
                     /locus_tag="IT904_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP3193160.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7221..7931
                     /gene="dsbC"
                     /locus_tag="IT904_003422"
     CDS             7221..7931
                     /gene="dsbC"
                     /locus_tag="IT904_003422"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAP3193161.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            7937..9670
                     /gene="recJ"
                     /locus_tag="IT904_003423"
     CDS             7937..9670
                     /gene="recJ"
                     /locus_tag="IT904_003423"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP3193162.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9761..10859
                     /gene="prfB"
                     /locus_tag="IT904_003424"
     CDS             join(9761..9835,9837..10859)
                     /gene="prfB"
                     /locus_tag="IT904_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP3193163.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10869..>11949
                     /gene="lysS"
                     /locus_tag="IT904_003425"
     CDS             10869..>11949
                     /gene="lysS"
                     /locus_tag="IT904_003425"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP3193164.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGI"
BASE COUNT         2853 a         2998 c         3230 g         2868 t
ORIGIN      
        1 gcactggcgc gaatatttac cctcaccccg gccctctccc tgaaagggcg agggggaaaa
       61 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
      121 taatttttct cggaaaaaca aatggctata gcacttgtga ctggtggcag tcgcggcatc
      181 gagcgggcaa ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag
      241 caaaacctcc acgcggcgca ggaagtgatg aacttaataa cgcaagccgg tggcaaagca
      301 ttcgtgctcc aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc
      361 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
      421 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg
      481 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
      541 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
      601 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
      661 gtcgccgcgc aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg
      721 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
      781 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
      841 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
      901 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
      961 ctggcaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
     1021 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggtg
     1081 gtgaacgaca cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
     1141 taagtcaccg cttttttcat ctcttcgata tgggcgcgca ggtagtcaat gcggtagtcg
     1201 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
     1261 aacagcggcc tctgataacg ctcatacagt tcgcaaagtg catagcgcag acctactgga
     1321 tcaatctgcc agccccagtc agatgcttta acatacgggt ttggtacgct gccttcaaaa
     1381 ccagagatcg catcgccggt gccgccttcg gccttcactg cattggtcat gtaatagctg
     1441 aaaccaagat aatcgcaggt gccttcacgc agcacatcca gatcgccgtc ttccattttg
     1501 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
     1561 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
     1621 gggttacagg agtaaggata gagcggcacc atcgccagca tacagccgac tttcatctcc
     1681 gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
     1741 acctgataca tcgtctcttc cgggttttca tgctcggtat acaccacgcc ggagcagcag
     1801 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
     1861 tatttgactt tgtgcttata gcgttcaaat accacttccg cgaaacgtac aaagaaatca
     1921 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
     1981 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
     2041 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
     2101 caggcaatgg atgtacgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
     2161 ttatagtgac cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact
     2221 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
     2281 ggcccttttc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
     2341 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
     2401 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
     2461 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
     2521 aacgggtgat gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga
     2581 agtcaccgca acctcaaccg taacgctgga tacgctgact gaaaaacatg cagagcagga
     2641 aaatatgacc ctgactgaac tgaaaaaagt cattgctgac atctatcccg gtcagacaca
     2701 attttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
     2761 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
     2821 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
     2881 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattca ctggcagagg
     2941 aaatagccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
     3001 tgctactggt tcaggcggtg gatcttaatg ccagcgccac agcgataacc agctacagcc
     3061 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
     3121 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
     3181 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
     3241 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
     3301 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
     3361 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg
     3421 tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca
     3481 tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata
     3541 ttgggcaggc gtaatgggtg tgcctttgta ggcatgataa gacgcgtcag cgtcgcatca
     3601 ggcatcggag cgcaaccgct ggatgcggcg tgaacgcctt atccgactta cgggcctcgc
     3661 ccctgttgtg ataaaggtat caaatcccag gcatgataag acgagtcagc gtcgcatcag
     3721 gcgataaatg cctgatgcga taaagagatt tactcttcga gcgaatacgg cagcggctcg
     3781 atatgcaatg tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta
     3841 ttcatgacga cttgtaccac gacctggcca tcttccagtt ttaccgcagc cagcaccgta
     3901 ccggtgcgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
     3961 cggctggcac tacctgtcag caaccagagc gcgcgtttgt tggcaccacg gaattttgct
     4021 cgcgccacca tctcttgtcc ggtatagcag cctttcttaa agctgatacc gcccagcgcc
     4081 tggaggttgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
     4141 ccagcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
     4201 agtttatcgg tcagcatatt ggcagtagct tcatcggtta cgatcaggaa acgttctgcc
     4261 ggatgttcaa accatagcag ggtagtcgca tcttctctga taacctgctt ttccttcgaa
     4321 ggtaatacac taaagaggtt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
     4381 agcacacgct cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt
     4441 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg
     4501 cggaacagac gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggccagcagg
     4561 tgctgatctt ctgtcatttg gctaacgtct gctgtcacct gaccctgcat atatttttcg
     4621 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag tgtcagtggc
     4681 aaacgggcag aagccgtagg ctgacggggc ggaaaaggtg taaaagccat aatagagtcc
     4741 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
     4801 ctttcactac gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agcccttgaa
     4861 ttgttgtgaa ttaatttcgc gatcgcgatt gtgtttactg atattcccgt aaaagaagtc
     4921 gaaaaacacg ttacaatagc ggggtactgg attttcgtga gacaggaaga cgatggacat
     4981 taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
     5041 aatcatgccg tttttcgaac atgagtatga cagcttaagc gatgacgaaa aacgcatctt
     5101 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
     5161 accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg
     5221 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc gcagtggctt tccttgctga
     5281 ttcatgggct ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt
     5341 tatggatggt gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgcatta
     5401 atgctcgcca gggggaaatt cgcctgttga tggacgggcg tttgcgttgg caagggcagg
     5461 agtggagcat cgtcaaagcg ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
     5521 ctgatagcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
     5581 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
     5641 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
     5701 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
     5761 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
     5821 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tagaaagttt gtcatgcagc
     5881 atccctagcg catcgaggaa ccattcaccg tacccgagtt gatcgccaag cccatacagc
     5941 gcaacaattt taccttcgag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
     6001 tcctggattt cgccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
     6061 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
     6121 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
     6181 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcggctagtg tatcagaatc
     6241 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
     6301 tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
     6361 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
     6421 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
     6481 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
     6541 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
     6601 cttcaccgaa attgccacag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
     6661 tattacaggc accattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
     6721 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
     6781 gcctacgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt
     6841 taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgt
     6901 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
     6961 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
     7021 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
     7081 tacgcgtggt gcagatgcta ctgggccaca gcgatctctc caccacgcaa atttatacgc
     7141 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
     7201 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
     7261 tttcaggctt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
     7321 tcaaaagcag cgatattcag cccgcgcctg tagctggcat gaagacagtt ctgactaaca
     7381 gcggcgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
     7441 ttagtggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
     7501 ttgaaaaaga gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta
     7561 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tacaacgcgc
     7621 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
     7681 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
     7741 caggtaaaag cgtcgcacca gccagttgcg acgtggatat tgccgaccat tacgcacttg
     7801 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
     7861 cgggttacca gccgccgaaa gagatgaaag aattcctcga cgaacaccaa aaaatgacca
     7921 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac
     7981 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt
     8041 acgcagtgcg caagaactgg aacgcagtgt taaaggtatg ctgccctggc agcaactgag
     8101 cggcgtcgaa aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat
     8161 tgtggtcggt gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat
     8221 gcgctcgctt ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta
     8281 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac
     8341 ggtggataac ggtatttcct cccatgcggg ggttgagcac gctcgctcgt tgggcatccc
     8401 ggttattgtt accgatcacc atttgccagg cgacacatta cccgcagcgg aagcgatcat
     8461 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
     8521 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
     8581 taacatcgca attcctaacc tggcagaact gctggatctg gtcgcgctgg ggacagtggc
     8641 ggacgtcgtg ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat
     8701 ccgagccgga aagtgccgtc cggggattaa agcgctgctt gaagtggcaa accgtgatgc
     8761 acaaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg
     8821 acgactggac gatatgtccg tcggtgtggc gctgttgttg tgcgacaaca tcggcgaagc
     8881 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
     8941 aatgcaaatt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc
     9001 cggcgggctg gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc
     9061 gcgcatcaaa gagcgttttc accgtccggt tatcgccttt gcgcccgcgg gtgatggtac
     9121 gctgaaaggt tctggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
     9181 agacacactc taccctggca tgatgctgaa gtttggcggt catgcgatgg cggcgggttt
     9241 gtcgctggaa gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
     9301 gtggctggac ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
     9361 cgaaatgacc atggaagtgg cgcagttgct gcgcgatgct ggcccgtggg ggcagatgtt
     9421 cccggagccg ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg
     9481 tcatttgaag gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt
     9541 taatgtcgat accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa
     9601 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
     9661 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
     9721 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
     9781 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
     9841 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
     9901 ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
     9961 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    10021 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    10081 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    10141 actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    10201 tgcttgagcg tatgtatctg cgctgggcag aatctcgtgg tttcaaaact gaaatcatcg
    10261 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    10321 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt
    10381 ttgactccgg cggtcgtcgc cacacatcgt tcagctctgc gtttgtttac ccggaagttg
    10441 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    10501 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    10561 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagaccagg
    10621 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    10681 aacaggcgat ggaagataac aaatccgaca tcggctgggg cagccagatt cgttcttatg
    10741 tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg
    10801 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    10861 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    10921 aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga
    10981 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggcaaagaga
    11041 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgtc
    11101 gtattatggg taaagcgtct ttcgttaccc tgcaggacgt tggcggtcgc attcagctgt
    11161 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    11221 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    11281 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    11341 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctcgatctc atctccaacg
    11401 atgaatcccg caacaccttt aaagtgcgct cgcagatcct ctctggtatt cgccagttca
    11461 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc cctggcggtg
    11521 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    11581 tcgcgccgga actgtacctc aagcgtctgg tggttggtgg cttcgagcgt gtattcgaaa
    11641 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    11701 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    11761 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    11821 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    11881 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    11941 tcggtatcc
//
LOCUS       DACAPB010000141        11911 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.141, whole genome shotgun sequence.
ACCESSION   DACAPB010000141 DACAPB010000000
VERSION     DACAPB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.141"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(66..2915)
                     /gene="rcsC"
                     /locus_tag="IT904_003426"
     CDS             complement(66..2915)
                     /gene="rcsC"
                     /locus_tag="IT904_003426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAP3193165.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMTNFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVARLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLSVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            3082..4908
                     /gene="atoS"
                     /locus_tag="IT904_003427"
     CDS             3082..4908
                     /gene="atoS"
                     /locus_tag="IT904_003427"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HAP3193166.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            4905..6290
                     /gene="atoC"
                     /locus_tag="IT904_003428"
     CDS             4905..6290
                     /gene="atoC"
                     /locus_tag="IT904_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HAP3193167.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            6486..7148
                     /gene="atoD"
                     /locus_tag="IT904_003429"
     CDS             6486..7148
                     /gene="atoD"
                     /locus_tag="IT904_003429"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HAP3193168.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            7148..7798
                     /locus_tag="IT904_003430"
     CDS             7148..7798
                     /locus_tag="IT904_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HAP3193169.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            7795..9117
                     /locus_tag="IT904_003431"
     CDS             7795..9117
                     /locus_tag="IT904_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HAP3193170.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            9148..10332
                     /locus_tag="IT904_003432"
     CDS             9148..10332
                     /locus_tag="IT904_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAP3193171.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(10406..11182)
                     /locus_tag="IT904_003433"
     CDS             complement(10406..11182)
                     /locus_tag="IT904_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAP3193172.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSSALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(11187..11888)
                     /locus_tag="IT904_003434"
     CDS             complement(11187..11888)
                     /locus_tag="IT904_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAP3193173.1"
                     /translation="MQSHLDREEYVARVLDREAKSTPPEAAKAMTVAIRTFLQQNANR
                     EGDCLTIPDSSATQRVSASPATTGARTMTAWTQDLIYAGDPVHYHGSRATEGTLSWRQ
                     ATAQAGQGERYDQILAFAYPDNSLSRWGAPRSTCQLLPKAKAWLANKMPQWRRILQGE
                     TGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRNFFTLQDRLDLTHEYLHLAFDGYPTGL
                     DENYIETLTRQLLMD"
BASE COUNT         2925 a         3136 c         2958 g         2892 t
ORIGIN      
        1 acgcgtcagc gtcgcatctg gcatttgcac tgaatgccag atgcggcata aacaccttat
       61 ccgtcctacg attcccgcga tttcctgacc ctctcggcat ataccgtcag cgtctgtttt
      121 atcacatcca gcgttaccgg cttcgacagg cagctgtcca taccggactc cagacaccgc
      181 tgcttctctt cagccaacgc attagcagtt actccgatta ccggcaacgt cagtcccaac
      241 tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg gcatgttgac gtcgctaagc
      301 acgatatcaa tatgattctt gctaagtaca ttaagcgcat cgacaccatc attcgcggtt
      361 ttacattgat agcccaacga tcccaactga tctgccagca gacgtcggtt aatcggatga
      421 tcatccacga ccagaatcat catatcgtca ttatcgctga ccgctttgtc cgtcgacggc
      481 agagcgttag caggatcgtc gctctccatc tcgatcaaat aaatacgcgc caacaatgcc
      541 ggtagctcat gcggggcagc cacactgtgt acccactccc ctggcgcttt ctccagcgga
      601 ataccaatat ggcgacgaca gaaggtcact accgctctac cctgccattt tttactcact
      661 acctcgtcag taattaacac atcttcggga gtcggttcct gcccttcgta tgttgtaacg
      721 acgatgccgc tgcgctgcaa actggtttcc aggaactgac agagcgacgc attgcggacc
      781 gacagccagc agcgtttacc actcaaccct tccacgcctt ttttctgcgg gtactgagcg
      841 ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc ccggttctga atctaccgag
      901 atatcgccgt ccatcatgct gatcagtttt tcacaaatcg ccagacccag accggtcccc
      961 tggaagtttc gctgtacacc cgttccgacc tggaagaagg gatcaaacaa gcgcaccact
     1021 tctttcgccg gaatccccac gccggtatcg cgaacacgga tagagagata atcgccatca
     1081 gcgcggacat gcaaaactat acagccggta tcggtgaatt ttatggcgtt actcaacagg
     1141 ttggagatga cctgctgtaa acgcatcggg tcgccattta aggccactgg cacatccggt
     1201 tcaataaagc agtacaagcc taactgcttg cgtaccacca gcggtaaata gttggcggtg
     1261 atgtggttca tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat
     1321 tcaatcttcg agaaatcgag aatatcgctg ataattttca acaacaggct ggaagagttg
     1381 ttcattgccg tcaccagacg agcgacgcct ttcggtaact ctttggtttg caacagatcc
     1441 aggttaccga taatgccata cagcggcgtt cgcagctcat gactgacggt ggcaaggaac
     1501 atcgattttg actggctcgc ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc
     1561 atcttcacgc gcgaagaaac atccaccagc acacaaatgg ccacgttttc attacgatag
     1621 cgcgaatgga cgaagctgat ttgcagattg gtattgttgc tggtcaggac atcaacaaaa
     1681 ttgacctgct gcccacagat aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata
     1741 ttgagatagg tatgcgccag ttcgttactt aaaatattga cgccatcagc ggtacgcaaa
     1801 atgcagatac ccactggcgc ggaggcgaca atcttgcgat tgaactgctc atgttcttcc
     1861 agtcgcaggg cgtcgctttc cgccggaatg aaaatacgtc gctcgtacat ccgtgcgaga
     1921 gtaaacaatg cagctccggc aagcacattc agcaaaattg cgttaaggat caacatgcga
     1981 atgcgttcca gcaccttatc aaccggcacc gaatacacga tgcttagcga tgagggtggc
     2041 agatttttct tcagcaccag ctcccggaac ccttccgtat agccaaacca ggagcgttcc
     2101 tgcatccagc gaggatcgcc cttaattttg ctttcaggtc cggtaagcga aatcagggta
     2161 tgaccatttt catcaagaat ggtaaccccc atcggcaacg tgcccggtaa gaaaaagttc
     2221 tccatccgga tggtctgctc gacacccaaa agcgcctgca accggttcgc cagataaact
     2281 ggcgtcaacg cgtaaaaata cccgacgcca gggcgcggac cttcgctgat ccagtagagg
     2341 ttactgccgc tatcatcttg tggtgcattt cgatatttat tgatgcgttc atgcaacgct
     2401 ttcaacgcgg tatcgcgttc cactggcata tcacgcagac cgaaattggt catgcagagg
     2461 ttatcgctgc cgattaaaaa tacccggttg agatcgtaag ccgcagaaaa attatcgcgc
     2521 cagtagcgca taaaccacgc caatgactcc agagaacctc gccaggtgtt actcattgcg
     2581 gaacaatcag agtcggcaaa cagcggttca aacgcaggca catccgcctg cgtttctcgt
     2641 ccacgcgggg aaagcacacc gttttctgcc gataagcgat tttcagcgat gtacttcagc
     2701 tctttcatca catcagaggt gcgttgaata aagcgctgag cctgatcgga actcagatta
     2761 aattcctgac gaatttccga ttctcgctga tgtaacgcat taacgatgta aaaaacggat
     2821 gaaaaagcaa tcaacagcca gagcactaac gccaatgctc tgaacatgta gcgcgaggct
     2881 ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg cgaagctccg cctcaggtga
     2941 ccgatggagt gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc tctcttctct
     3001 ggaatatgat acaccgccga gaaatcatca ccttaacctc tgataatcgt catataccgg
     3061 acaagactag tggatttcag catgcattat atgaagtgga tttatccacg ccgcttacgc
     3121 aatcaaatga tcctgatggc aatcctgatg gtcattgtcc caacgcttac tattggttat
     3181 atcgtagaaa cggaaggacg ttcagcagtc ttatctgaaa aagagaaaaa actttctgcc
     3241 gtggtcaacc tgcttaatca ggcactaggc gatcgctatg atctctacat cgacttacca
     3301 cgtgaggagc gtatccgcgc attaaatgca gaacttgccc ccattaccga aaatatcact
     3361 cacgccttcc ctggcatcgg tgctggttat tacaacaaaa tgctggatgc gataatcacc
     3421 tacgcgcctt cagcgctata tcagaataat gtcggcgtta ccattgccgc agatcaccct
     3481 ggtcgcgaag tcatgcgtac aaatacccct ttggtttatt caggcaggca ggtgcgcggc
     3541 gatattttga attcaatgct ccccattgag cgtaatggtg aaatcctcgg ctatatctgg
     3601 gccaatgaat taaccgaaga tattcgccgc caggcctgga aaatggatgt gaggattatc
     3661 attgtgctca ccgccggttt gctgataagc ctgctattga ttgtcctttt ctcccgtcgc
     3721 ctgagcgcca atattgatat catcaccgat ggcctctcga ctctggcaca aaatattccc
     3781 actcgattac cacaattgcc cggtgaaatg gggcaaatca gtcagagtgt taataacctc
     3841 gcccaggcac tgcgtgaaac gcggacactt aacgatctga ttattgaaaa cgctgccgat
     3901 ggcgtcattg ccattgaccg ccagggtgat gtaaccacca tgaacccagc agcagaagtt
     3961 atcactggct atcaacgcca tgaactggta gggcagcctt actccatgtt gttcgacaat
     4021 actcagttct acagtccagt actggatacg ctggaacatg gcaccgaaca tgtggcgctg
     4081 gagatcagtt ttccaggtcg tgaccgcacc attgaactca gtgtcactac cagtcgtatt
     4141 cataacacgc acggtgaaat gataggtgct ttggtgattt tctctgattt aactgcccgc
     4201 aaagaaaccc agcgccgcat ggcgcaagca gaacgcctcg ccacactggg tgagctgatg
     4261 gctggcgtcg cgcatgaagt acgtaatccg ttaacggcta ttcgtggtta tgtacagatc
     4321 ttgcgccaac aaaccagtga cccaatacat caggaatatc tgtccgtagt actcaaagaa
     4381 atcgattcaa ttaacaaagt tattcagcaa ttgctcgaat tttcacgtcc acgccacagt
     4441 caatggcaac aagtcagcct caatgcattg gttgaagaaa ctctggtact ggtacaaacc
     4501 gccggcgtac aagcgcgggt cgacttcata agcgaactgg ataatgaatt aagcccgatt
     4561 aacgccgatc gtgaactgct caaacaggta ctactgaata tcctgatcaa tgccgtccag
     4621 gctatcagcg cacgagggaa aattcgcatt caaacctggc aatacagcga ctcacaacag
     4681 gccatttcga tagaggacaa cggctgtggc attgatctct cgctgcaaaa aaagatcttc
     4741 gatccctttt tcaccaccaa agcctcagga accgggcttg gtctggcgtt aagtcaacgc
     4801 atcattaatg cccatcaggg tgatattcgc gtcgccagtt tgccgggcta cggcgcaacc
     4861 ttcacgctta ttttaccgat caacccgcag ggaaatcaga ctgtatgact gctattaatc
     4921 gcatccttat tgtggatgat gaagataatg ttcgccgtat gctaagcacc gcttttgcac
     4981 tacaaggatt cgaaacacat tgtgcgaaca acggacgcac agcattacac ctgtttgccg
     5041 atattcaccc tgatgtggtg ttgatggata tccgcatgcc agagatggac ggcatcaagg
     5101 cactaaagga gatgcgcagc catgagaccc ggacacccgt tattctgatg acggcctatg
     5161 cggaagtgga aaccgccgtc gaagcgctac gctgcggagc cttcgactat gttattaaac
     5221 cgtttgatct cgatgagttg aatttaatcg ttcagcgcgc tttacaactc cagtcaatga
     5281 aaaaagagat ccgtcatctg caccaggcac tgagcaccag ctggcaatgg gggcacattc
     5341 tcaccaacag cccggcgatg atggacatct gcaaagacac cgccaaaatt gccctttctc
     5401 aggccagcgt cttgattagc ggtgaaagcg gcaccgggaa agagttgatt gccagagcga
     5461 ttcactacaa ttcgcggcgg gcaaaggggc cgttcattaa agtcaactgc gcggcgctgc
     5521 cggaatcgtt gctcgaaagt gaactgtttg gtcatgaaaa aggtgcattt actggtgcac
     5581 aaaccttgcg tcagggatta tttgaacgag ccaacgaagg tactctgctc ctcgacgaaa
     5641 ttggcgaaat gccgctggta ctacaagcca aattactacg cattctacag gaacgggaat
     5701 ttgaacggat tggcggccat cagaccataa aagttgatat ccgcatcatt gctgccacca
     5761 accgtgactt gcaggcaatg gtaaaagaag gcaccttccg tgaagatctc ttttatcgcc
     5821 ttaacgttat tcatttaata ctgccgcctc tgcgcgatcg ccgggaagat atttccctgt
     5881 tagctaatca ctttttgcaa aaattcagta gtgagaatca gcgcgatatt atcgacatcg
     5941 atccgatggc aatgtcactg cttaccgcct ggtcatggcc gggaaatatt cgagagcttt
     6001 ccaacgttat tgaacgcgcc gtcgtgatga attcaggccc gatcattttt tctgaggatc
     6061 ttccgccaca gattcgtcag ccagtctgta atgctggcga ggtaaaaaca gcccctgtcg
     6121 gtgagcgtaa tttaaaagag gaaattaaac gcgtcgaaaa acgcatcatt atggaagtgc
     6181 tggaacaaca agaaggaaac cgaacccgca ctgctttaat gctgggcatc agtcgccgtg
     6241 cattgatgta taaactccag gaatacggta tcgatccggc ggatgtataa caccaaaact
     6301 tgctatgcag aaatttgcac agtgcgcaat tttctgcata gccgctcatt ctccttataa
     6361 atccccatcc aatttatccc ttcatattca attagttaaa taactaaatc caataatctc
     6421 attctggcac tccccttgct attgcctgac tgtacccaca acggtgtatg caagagggat
     6481 aaaaaatgaa aacaaaattg atgacattac aagacgccac cggcttcttt cgtgacggca
     6541 tgaccatcat ggtgggcgga tttatgggga ttggcactcc atcccgcctg gttgaagcat
     6601 tactggaatc tggtgttcgc gacctgacat tgatagccaa tgataccgcg tttgttgata
     6661 ccggcatcgg tccgctcatc gtcaatggtc gagtccgcaa agtgattgct tcacatatcg
     6721 gcaccaaccc ggaaacaggt cggcgcatga tatctggtga gatggacgtc gttctggtgc
     6781 cgcaaggtac gctaatcgag caaattcgct gtggtggagc tggacttggt ggttttctca
     6841 ccccaacggg tgtcggcacc gtcgtagagg aaggcaaaca gacactgaca ctcgacggta
     6901 aaacctggct gctcgaacgc ccactgcgcg ccgacctagc gctaattcgc gctcatcgtt
     6961 gcgacacact tggcaacctg acctatcaac ttagcgcccg caactttaac cccctgatag
     7021 cccttgcggc tgatatcacg ctggtagagc cagatgaact ggtcgaaacc ggcgagctgc
     7081 aacctgacca tattgtcacc cctggtgccg ttatcgacca catcatcgtt tcacaggaga
     7141 gcaaataatg gatgcgaaac aacgtattgc gcgccgtgtg gcgcaagagc ttcgtgatgg
     7201 tgacatcgtt aacttaggga tcggtttacc cacaatggtc gccaattatt taccggaggg
     7261 tattcatatc actctgcaat cggaaaacgg cttcctcggt ttaggcccgg tcacgacagc
     7321 gcatccagat ctggtgaacg ctggcgggca accgtgcggt gttttacccg gtgcagccat
     7381 gtttgatagc gccatgtcat ttgcgctaat ccgtggcggt catattgatg cctgcgtgct
     7441 cggcggtttg caagtagacg aagaagcaaa cctcgcgaac tgggtagtgc ctgggaaaat
     7501 ggtgcccggt atgggtggcg cgatggatct ggtgaccggg tcgcgcaaag tgatcatcgc
     7561 catggaacat tgcgccaaag atggttcagc aaaaattttg cgccgctgca ccatgccact
     7621 cactgcgcaa catgcggtgc atatgctggt tactgaactg gctgtctttc gttttattga
     7681 cggcaaaatg tggctcaccg aaattgccga cgggtgtgat ttagccaccg tgcgtgccaa
     7741 aacagaagct cggtttgaag tcgccgccga tctgaatacg caacggggtg atttatgatt
     7801 ggtcgcatat cgcgttttat gacgcgtttt gtcagccggt ggcttcccga tccactgatc
     7861 tttgccatgt tgctgacatt gctaacattc gtgatcgcgc tttggttaac accacaaacg
     7921 ccgatcagca tggtgaaaat gtggggtgac ggtttctgga acttgctggc gtttggtatg
     7981 cagatggcgc ttatcatcgt taccggtcat gcccttgcca gctctgctcc ggtgaaaagt
     8041 ttgctgcgta ctgccgcctc cgccgcaaag acgcccgtac agggcgtcat gctggtcact
     8101 ttcttcggtt cagtcgcttg tgtcatcaac tggggatttg gtttggttgt cggcgcaatg
     8161 tttgcccgtg aagtcgcccg gcgagtcccc ggttctgatt atccgttgct cattgcctgc
     8221 gcctacattg gttttctcac ctggggtggc ggcttctctg gatcaatgcc tctgttggct
     8281 gcaacaccgg gcaacccggt tgagcatatc gccgggctga tcccggtggg cgatactctg
     8341 ttcagtggtt ttaacatttt catcactgtg gcgttgattg tggtgatgcc atttatcacc
     8401 cgcatgatga tgccaaaacc gtctgacgtg gtgagtatcg atccaaaact actcatggaa
     8461 gaggctgatt ttcaaaagca gctaccgaaa gatgccccac catccgagcg actggaagaa
     8521 agccgcattc tgacgttgat catcggcgca ctcggtatcg cttaccttgc gatgtacttc
     8581 agcgaacatg gcttcaacat caccatcaat accgtcaacc tgatgtttat gattgcgggt
     8641 ctgctgctac ataaaacgcc aatggcttat atgcgtgcta tcagcgcggc agcacgcagt
     8701 actgccggta ttctggtgca attccccttc tacgctggga tccaactgat gatggagcat
     8761 tccggtctgg gcggactcat taccgaattc ttcatcaatg ttgcgaacaa agacaccttc
     8821 ccggtaatga ccttttttag ttctgcactg attaacttcg ccgttccgtc tggcggcggt
     8881 cactgggtta ttcagggacc tttcgtgata cccgcagccc aggcgctggg cgctgatctc
     8941 ggtaaatcgg taatggcgat cgcctacggc gagcaatgga tgaacatggc acaaccattc
     9001 tgggcgctgc cagcactggc aatcgccgga ctcggtgtcc gcgacatcat gggctactgc
     9061 atcactgccc tgctcttctc cggtgtcatt ttcgtcattg gtttaacgct gttctgacgg
     9121 cacccctaca aacagaagga atataaaatg aaaaattgtg tcatcgtcag tgcggtacgt
     9181 actgctatcg gtagttttaa cggttcactc gcttccacca gcgccatcga cctgggggcg
     9241 acagtaatta aagccgccat tgaacgtgca aaaatcgatt cacaacacgt tgatgaagtg
     9301 attatgggta acgtgttaca agccgggctg gggcaaaatc cggcgcgtca ggcactgtta
     9361 aaaagcgggc tggcagaaac ggtgtgcgga ttcacggtca ataaagtatg tggttcgggt
     9421 cttaaaagtg tggcgcttgc cgcccaggcc attcaggcag gtcaggcgca gagcattgtg
     9481 gcggggggta tggaaaatat gagtttagcc ccctacttac tcgatgcaaa agcacgctct
     9541 ggttatcgtc ttggagacgg acaggtttat gacgtaatcc tgcgcgatgg cctgatgtgc
     9601 gccacccatg gttatcatat ggggattacc gccgaaaacg tggctaaaga gtacggaatt
     9661 acccgtgaaa tgcaggatga actggcgcta cattcacagc gtaaagcggc agccgcaatt
     9721 gagtccggtg cttttacagc cgaaatcgtc ccggtaaatg ttgtcactcg gaagaaaacc
     9781 ttcgtcttca gtcaagacga attcccgaaa gcgaattcaa cggctgaagc gttaggtgca
     9841 ttgcgcccgg ccttcgataa agcaggaaca gtcaccgctg ggaacgcgtc tggtattaac
     9901 gacggtgctg ccgctctggt gattatggaa gaatctgcgg cgctggcagc aggccttacc
     9961 cccctggctc gcattaaaag ttatgccagc ggtggcgtgc cccccgcatt gatgggtatg
    10021 gggccagtac ctgccacgca aaaagcgtta caactggcgg ggctgcaact ggcggatatt
    10081 gatctcattg aggctaatga agcatttgct gcacagttcc ttgccgttgg gaaaaccctg
    10141 ggctttgatt ctgagaaagt gaatgtcaac ggcggggcca tcgcgctcgg gcatcctatc
    10201 ggtgccagtg gtgctcgtat tctggtcaca ctattacatg ccatgcaggc acgcgataaa
    10261 acgctggggc tggcaacact gtgcattggc ggcggccagg gaattgcgat ggtgattgaa
    10321 cggttgaatt aatcaataaa aacacccgat agcgaaagtt atcgggtgtt ttcttgaaca
    10381 tcgacggcga aggtaacccc attaatcacc agtcaaaact tttcaccagc gtcagctcgc
    10441 cagcattacg catcggtaca ataaatgttt cctgtttctc attgaccgat ccttcatcgg
    10501 tgatcagcgt cagttgggcg gtggttaatt ccgtttcgct gcgcccacca tagtagttga
    10561 tatacacctg atagcgcccg tgaattggcg cgggcatggc gaaaatctcg ggtccgtacc
    10621 ccgtcgtgac atccatatcc agtgcactac tgtttttcag cacggtgtta ccgtaccagg
    10681 cgtgttcgcc atcgggcgtg acaacatgga gatcgagatc ggtattgtcc gtatcccacg
    10741 agagaaccag ccgtaaacgt gcacgaatcg ttcctgtacc cggcgttgag taaaactgca
    10801 ttttttgtcg gctttgccca tccgggctga tgacctgcac gctgttgctg ccttcagtga
    10861 aaatataagg gcgtgcaaat gaaccatcgg attcaattct ctgcggcata ctggcaccgt
    10921 tgaccaccag ccggccctgc tgaactttac ccgccgcagc ataattcttt attttgccgc
    10981 ggatctgcgc tgagatattt tgatcatctg ccatgttgac ggacgacgcc gggtaattga
    11041 tcgattgtga aaaactggca tcttcgccct ctgccggatg ccagcctgag agcggtgcat
    11101 caatctcgac ttcctgcaca ccttcactgt gagcgacagg cgaaagcgcc accagtaaca
    11161 acggaagaaa aatctttcgc atagcattag tccattaata attgtcgggt cagcgtttcg
    11221 atatagttct catcaagccc ggtgggataa ccatcaaagg ccaggtgcag atactcatgg
    11281 gttaaatcca gccgatcctg aagcgtaaag aagttacgaa taaacagccg tttctgctgc
    11341 cgatcggtat aggggaaacc tgagactaaa cgacagaccg caaacacgtc tggttcgttg
    11401 taccctgtct cgccttgtaa tatacgccgc cactgcggca ttttattcgc cagccaggct
    11461 ttcgctttgg gtaataactg gcaggttgag cgtggcgctc cccagcggct aagactgttg
    11521 tcgggataag caaacgcgag tatctggtcg taacgctctc cctgccccgc ctgggccgtg
    11581 gcttgccgcc aggaaagtgt cccttcggta gcgcgactgc catgataatg aaccggatcg
    11641 cctgcataga tgagatcctg cgtccaggcg gtcatcgttc gcgcccccgt ggtcgctggc
    11701 gaagcagaaa cacgctgcgt ggcgctgctg tcggggatgg tcaggcaatc gccttcccga
    11761 ttggcgtttt gctgtaaaaa agtgcgaatc gccacggtca tggctttcgc tgcctcaggg
    11821 ggcgtacttt tcgcctcgcg atccagaact ctggcaacgt actcttcacg atcgagatgc
    11881 gactgcaatt tcagtttgcc cttttcgctc a
//
LOCUS       DACAPB010000142        11853 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.142, whole genome shotgun sequence.
ACCESSION   DACAPB010000142 DACAPB010000000
VERSION     DACAPB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.142"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..169
                     /locus_tag="IT904_003435"
     CDS             <1..169
                     /locus_tag="IT904_003435"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="catalyzes the removal of elemental sulfur from
                     cysteine to produce alanine; involved in NAD biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HAP3193174.1"
                     /translation="HSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQG
                     VDLNSIEWAHH"
     gene            197..583
                     /gene="iscU"
                     /locus_tag="IT904_003436"
     CDS             197..583
                     /gene="iscU"
                     /locus_tag="IT904_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAP3193175.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            600..923
                     /gene="iscA"
                     /locus_tag="IT904_003437"
     CDS             600..923
                     /gene="iscA"
                     /locus_tag="IT904_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAP3193176.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            1019..1534
                     /gene="hscB"
                     /locus_tag="IT904_003438"
     CDS             1019..1534
                     /gene="hscB"
                     /locus_tag="IT904_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAP3193177.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            1551..3401
                     /gene="hscA"
                     /locus_tag="IT904_003439"
     CDS             1551..3401
                     /gene="hscA"
                     /locus_tag="IT904_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAP3193178.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            3403..3738
                     /gene="fdx"
                     /locus_tag="IT904_003440"
     CDS             3403..3738
                     /gene="fdx"
                     /locus_tag="IT904_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAP3193179.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            3750..3950
                     /gene="iscX"
                     /locus_tag="IT904_003441"
     CDS             3750..3950
                     /gene="iscX"
                     /locus_tag="IT904_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAP3193180.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            4008..5291
                     /gene="pepB"
                     /locus_tag="IT904_003442"
     CDS             4008..5291
                     /gene="pepB"
                     /locus_tag="IT904_003442"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAP3193181.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            5433..6209
                     /gene="sseB"
                     /locus_tag="IT904_003443"
     CDS             5433..6209
                     /gene="sseB"
                     /locus_tag="IT904_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAP3193182.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(7027..7872)
                     /gene="sseA"
                     /locus_tag="IT904_003444"
     CDS             complement(7027..7872)
                     /gene="sseA"
                     /locus_tag="IT904_003444"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAP3193183.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            8079..>11853
                     /gene="yfhM"
                     /locus_tag="IT904_003445"
     CDS             8079..>11853
                     /gene="yfhM"
                     /locus_tag="IT904_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HAP3193184.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHLTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVM"
BASE COUNT         2928 a         2917 c         3285 g         2723 t
ORIGIN      
        1 acatagctct atccgtttct ctttaggtcg ttttactact gaagaagaga tcgactacac
       61 catcgagtta gttcgtaaat ccatcggtcg tctgcgtgac ctttctccgc tgtgggaaat
      121 gtacaagcag ggcgtggatc tgaacagcat cgaatgggct catcattaat cggtatcgga
      181 atcaggagaa tttataatgg cttacagcga aaaagttatc gaccattacg agaatccgcg
      241 taacgtgggt tcctttgaca acaacgacga gaacgtcggc agcggcatgg tgggtgcacc
      301 ggcctgtggc gacgtgatga agttgcagat taaagtcaac gatgaaggta tcattgaaga
      361 cgcgcgtttt aaaacttacg gctgcggttc cgctatcgct tccagctccc tggtcaccga
      421 atgggtgaaa gggaagtctc tcgacgaagc gcaggcgatc aaaaacaccg atattgctga
      481 agaacttgaa ctgccgccgg tgaaaattca ctgttctatt ctggcagaag acgcgatcaa
      541 agccgccatt gcggactata aaagcaaacg tgaagcaaaa taagagttga ggtttggtta
      601 tgtcgattac actgagcgac agtgcagcag cgcgagtaaa tacctttctg gctaaccgcg
      661 gtaaagggtt tggcctgcgt ctgggcgtga gaacctccgg gtgttcaggt atggcttatg
      721 tactggaatt tgttgacgaa ccgacgccgg aagacatcgt gtttgaagac aaaggcgtga
      781 aagtcgtggt cgatggcaag agcctgcaat ttctggacgg tacgcagctg gacttcgtaa
      841 aagaaggcct gaacgaaggg tttaaattca ccaacccgaa cgtcaaagat gagtgtggtt
      901 gcggcgaaag cttccacgtt tgatgcgcat accgataacc ccaccgtggt cgcctgcgcg
      961 tggggtttgt tttacctgat tcgccgttat cgcggcggat cgcagccctg agaatgttat
     1021 ggattacttc accctctttg gcttgcctgc ccgctatcaa ctcgataccc aggcgctgag
     1081 cctgcgtttt caggatctac aacgtcagta tcatcctgat aaattcgcca gcggaagcca
     1141 ggcggaacaa ctcgccgccg tacagcaatc tgcaaccatt aaccaggcct ggcaaacgct
     1201 gcgtcatccg ttaatgcgcg cggaatattt gctttctttg cacggctttg atctcgccag
     1261 cgagcagcat actgtgcgcg acaccgcgtt cctgatggaa cagttggagc tgcgcgaaga
     1321 gctggacgag atcgaacagg cgaaagatga agcgcggctg gaaagcttta tcaaacgtgt
     1381 gaaaaagatg tttgataccc gccatcagtt gatggttgaa cagttagaca acgagacgtg
     1441 ggacgcggcg gcggataccg tgcgtaagct gcgttttctc gataaactgc gaagcagtgc
     1501 cgaacaactc gaagaaaaac tgctcgattt ttaatttctg gaagctaaac atggccttat
     1561 tacaaattag tgaacctggt ttgagtgctg cgccgcatca gcgtcgtctg gcggccggta
     1621 ttgacctggg cacaaccaac tcgctggtgg cgacagtgcg cagcggtcag gccgaaacgt
     1681 tagccgatca tgaaggccgt cacctgctgc catctgttgt tcactatcaa cagcaagggc
     1741 attcggtggg ttatgacgcg cgtactaatg cagcgctcga taccgccaac acaattagtt
     1801 ctgttaaacg cctgatggga cgctcgctgg ctgatatcca gcaacgctat ccgcatctgc
     1861 cttatcaatt ccaggccagc gaaaacggcc tgccgatgat tgaaacggcg gcggggctgc
     1921 tgaacccggt gcgcgtttct gcggacatcc tcaaagcact ggcggcgcgg gcaactgaag
     1981 ccctggcagg cgagctggat ggtgtagtta tcaccgttcc ggcgtacttt gacgatgccc
     2041 agcgtcaggg caccaaagac gcggcgcgtc tggcgggcct tcacgtcctg cgcttactta
     2101 acgaaccgac cgctgcggct atcgcctacg ggctggattc cggtcaggaa ggcgtgatcg
     2161 ccgtttatga cctcggtggc gggacgtttg atatttccat tctgcgctta agtcgcggcg
     2221 tgtttgaagt gctggcaacc ggcggtgatt ccgcgctcgg cggcgatgat ttcgaccatc
     2281 tgctggcgga ttacattcgc gagcaggcgg gcattcctga tcgtagcgat aaccgcgttc
     2341 agcgtgaact gctggatgcc gccattgcag ccaaaatcgc gctgagcgat gcggactccg
     2401 tgaccgttaa cgttgcgggc tggcagggcg aaatcagccg tgaacaattc aatgaactga
     2461 tcgcgccact ggtaaaacga accttactgg cttgtcgtcg cgcgctgaaa gacgcgggtg
     2521 tagaagctga tgaagtgctg gaagtggtga tggtgggcgg ttctactcgc gtgccgctgg
     2581 tgcgtgaacg ggtaggcgaa tttttcggtc gtccaccgct gacttccatc gacccggata
     2641 aagtcgtcgc tattggcgcg gcgattcagg cggatattct ggtgggtaac aagccagaca
     2701 gcgaaatgct gttgcttgat gtgatcccac tgtcgctggg cctcgaaacg atgggcggcc
     2761 tggtggagaa agtgattccg cgtaatacca ctattccggt ggcccgcgct caggatttca
     2821 ccacctttaa agatggtcag acggcgatgt ctatccatgt aatgcagggt gagcgcgaac
     2881 tggtgcagga ctgccgctca ctggcgcgtt ttgcgctgcg tggtattccg gcgctaccgg
     2941 ctggcggtgc gcatattcgc gtgacgttcc aggtcgatgc cgacggtctt ttgagcgtga
     3001 cggcgatgga gaaatccacc ggcgttgagg cgtctattca ggtcaaaccg tcttacggtc
     3061 tgaccgatag cgaaatcgct tcgatgatca aagactcaat gagctatgcc gagcaggacg
     3121 taaaagcccg aatgctggca gaacaaaaag tagaagcggc gcgtgtgctg gaaagtctgc
     3181 acggcgcgct ggctgctgat gccgcgctgt taagcgccgc agaacgtcag gtcattgacg
     3241 atgctgccgc tcacctgagt gaagtggcgc agggcgatga tgttgacgcc atcgaacaag
     3301 cgattaaaaa cgtagacaaa caaacccagg atttcgccgc tcgccgcatg gaccagtcgg
     3361 ttcgtcgtgc gctgaaaggc cattccgtgg acgaggttta atatgccaaa gattgttatt
     3421 ttgcctcatc aggatctctg ccctgatggc gctgttctgg aagctaatag cggtgaaacc
     3481 attctcgacg cagctctgcg taacggtatc gagattgaac acgcctgtga aaaatcctgt
     3541 gcttgcacca cctgccactg catcgttcgt gaaggttttg actcactgcc ggaaagctca
     3601 gagcaggaag acgacatgct ggacaaagcc tggggactgg agccggaaag ccgtttaagc
     3661 tgccaggcgc gcgttaccga cgaagattta gtagtcgaaa tcccgcgtta cactatcaac
     3721 catgcgcgtg agcattaaca gaggttagta tgggacttaa gtggaccgat agccgcgaaa
     3781 ttggcgaagc actgtacgat gcgtatcccg atcttgatcc gaaaacggtt cgattcaccg
     3841 atatgcatca gtggatttgc gatctggaag atttcgacga cgacccgcag gcatccaacg
     3901 agaaaatcct agaagcgatt ttgttagtct ggctggacga ggccgaatga ataaacgggc
     3961 tgcctggggt agcccgtttt gctgttaaac gaataaggat aactaaaatg acagaagcga
     4021 tgaaaattac cctctctacc caacctgccg acgcgcgctg gggagaaaaa gcaacttaca
     4081 gcattaataa tgatggcatt accctgcatt tgaacggggc agacgatctg gggctgatcc
     4141 agcgtgcggc ccgcaagatt gacggtctgg gcatcaagca tgttcagtta agcggtgaag
     4201 gttgggatgc ggatcgctgc tgggcattct ggcaaggtta caaagccccg aaaggcacgc
     4261 gtaaagtgga gtggccggat ctggacgatg cccagcgcca ggaactggat aaccgcctga
     4321 tgatcatcga ctgggtgcgt gacaccatca acgcaccggc agaagaattg ggaccatcgc
     4381 aactggcaca gcgtgctgtt gatctgatca gcaacgtcgc gggcgatcgt gtgacttatc
     4441 ggatcaccaa aggcgaagat ctgcgtgagc aaggttatat ggggctgcac accgtcggac
     4501 gcggttcaga acgttctccg gtattgctgg cgctggatta caacccaact ggcgataaag
     4561 aagcgccagt gtacgcgtgc ctggtaggta aaggtatcac ttttgactcc ggcggctaca
     4621 gcatcaaaca gactgcgttt atggactcga tgaagtcgga catgggcggc gcggcaacgg
     4681 ttaccggggc gctggcattt gccattacgc gcggactgaa caagcgcgtg aagctgttcc
     4741 tctgctgtgc ggataacctg attagcggca atgcgttcaa gctgggcgat atcatcacct
     4801 atcgcaacgg taaaaaagtt gaagtgatga acactgatgc cgaagggcgt ctggtgcttg
     4861 ccgatggtct gattgatgcc agtgcgcaga aaccggaaat gatcattgat gcggcgaccc
     4921 tcaccggggc ggcgaaaact gcgctgggta atgattatca cgcgctgttc agttttgacg
     4981 atgcgctggc cggtcgcttg ctggcgagtg ccgcgcagga gaacgaaccg ttctggcgtc
     5041 tgccgctggc ggagttccac cgcagccagc tgccgtctaa ctttgccgaa ctgaacaata
     5101 ccggaagcgc ggcgtatccg gcaggcgcga gcacggcggc gggcttcctg tcgcactttg
     5161 ttgagaacta tcagcaaggc tggctgcata tcgactgctc ggcgacttac cgtaaagcgc
     5221 cggttgaaca gtggtctgcg ggcgctacgg gacttggtgt gcgcacgata gctaatctgt
     5281 taacggcgta aactaatatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct
     5341 gcaaattcaa caaattgcga atcccttgta ggcctgataa gcgtagcgca tcaggcattt
     5401 gcgccccgaa cgttgaatga attgtggttc ttatgtccga aacaaaaaac gaacttgaag
     5461 acctgctgga aaaagcagca actgaaccgg cgcaccgccc ggcctttttc cgtactctac
     5521 tggaatccac cgtctgggtg cctggtacgg cggcgcaggg cgaggctgtg gttgaagata
     5581 gcgcgcttga tttacagcac tgggaaaaag aagacggcac cagcgtcatt ccttttttca
     5641 cctcgttaga agcacttcaa caggcggttg aagacgaaca ggcatttgtc gtaatgcccg
     5701 ttcgcacgct gtttgagatg acacttggcg aaacgctctt ccttaatgcc aaactgccaa
     5761 ccggtaaaga attcatgccg cgtgaaatca gtttgttgat tggtgaagag ggaaatccgc
     5821 tgagcagcca ggaaatcctg gaaggcggtg aatcgctgat attatcggaa gtcgcagagc
     5881 cgccagcaca aatgattgat tcactcacca ccttatttaa aaccattaag ccggtgaagc
     5941 gtgcttttat ttgttcaatt aaagagaacg aagaggcaca gcctaattta cttattggca
     6001 ttgaagccga tggtgatatc gaagaaatta ttcaggcgac gggaagtgta gcgaccgata
     6061 cattacctgg cgatgaacca atcgatattt gtcaggtgaa aaaaggggaa aaaggaatta
     6121 gccactttat taccgaacat attgcgccat tctatgaacg tcgctggggt ggttttttgc
     6181 gtgactttaa acagaatcgg ataatctaaa atatgccgcc caaagggcgg cattcgggct
     6241 tacttgctgc tctgaaggtt gttaaccacc agtaaattgt cgccggagag actccggtta
     6301 ttatttacct cggttaacag cgcaccagaa acaataagta cgatacaaaa gcaccgtatc
     6361 ttcattgtgc ggtccatgcg aagaccgcat cacgacagca ccactggcga tattgccgcg
     6421 atagcaagcg ctgtgaaaca cctgcattta cggccacgga acatcctttc ctaaagccag
     6481 cgcccgtttg cgagacggcg gaaagggccg cagtggataa tacactaaca gaacaataat
     6541 aactctcttt tgcttgacaa aaagagagtt actggcgagt attgtctccg ctgagacgcg
     6601 cttgcattac tacggtaata caacctgctg gaaaggccgc gaaccggacc agcaacaaaa
     6661 aaccgcctga ttttggcggt tttttgtttt ttaaaggaaa gtgaatttac atgtttaagt
     6721 aatatctttt accggttaga atttttaatt ttgagcagga tttgtttata tttgtctgaa
     6781 ttcggactaa taagccaggt tattttatta ccgaacacgg cggaaagggc cggtgtcgat
     6841 aatacgctaa ataaacaata attactctct tttgcttgac aaaaagagag ttactggtga
     6901 gtattgtttt gctgtgaagc acctgcgttg ctcatgtaac acaagtcaac ctgctggaaa
     6961 ggccgcgaac cagactagca ataaaaaacc gccaaattcg gcggtttttt attgcctgta
     7021 aaatacttat ttcactggct caaccggtaa atctgcccgc gcgccccatt cactccatgc
     7081 gccgtcgtac agtttcacgt ttggcacatc cagcgtcgcg agtgctaaca aaaccacggc
     7141 tgccgttaca ccagagccgc agctgacgat aattggtttg tcgtagctga cgccgcgacc
     7201 aaaaaatatc gcatccagtt catcggtcgt ttttagttcg ccttcgcgca ccagttccgt
     7261 ccacggaaca ttcagtgctc cgggaatatg tccgcgacgt aaacctgggc gaggttcatc
     7321 aacttctgcg ttaaaacgtg cagccgggcg ggcatcaata atttgcgccg tattttcatg
     7381 gcttgccaat aatacatcgg ttactttcac cacggcttca ggattaaacg cggcgttaaa
     7441 ctctccttcc ggcagctcta ctgcaccttc ttctaacagc agatcatcgc gctgccagcc
     7501 tgcaagtcca ccccccagaa tcgacacttt ctctacacca aaggtgcgca gcatccacca
     7561 tgctcgtggg gctgagaaaa gattaccttc gtcatagaca atcaggtgct tatcctggtt
     7621 aacgcctaat tcacgcatcg ccacggcgaa cgtttccggg cgcggcagca tgtgcggaag
     7681 cggggaagtg tgatcagaaa gcgcttcgat atcaaaaaac actgcgccgg gaatatgtcc
     7741 attcagatac tcctgagcaa cgttacgatc ctcctgtcca ggcgacgcca tgcgggcatc
     7801 gataatctga atttccgggt catcaatatg ttcggcgagc cagtcggctc ctacaaacca
     7861 tgtcgtggac ataggcatct ccatttttgc cggggttagg ctaaattgtc ggatgtttag
     7921 tgaaaagcga caagcaaaag aggctgattt ggctgtctga gccacaattc tcactaaaat
     7981 tcggattgta tgggcgtgat actgaattac ataacgtaaa ttctcacgca taataattat
     8041 tcgacgttgc taacgggcca ttgtggcagg gatgaaaaat gaaaaagtta cgcgtagccg
     8101 cctgcatgct aatgctggcg ctggcagggt gcgacaacaa cgataacgcg ccaacagcgg
     8161 tgaaaaaaga tgcgccttct gaagttacta aagcggcctc ttcagaaaac gcgagttcag
     8221 caaaactctc cgcgccagag cgacaaaaac tggcacaaca gagtgccggt aaggcgctga
     8281 cattgctgga tctctctgaa gtccaacttg atggtgcagc cacgctggtg ctgacgttct
     8341 ccatccctct cgacccggat caggatttct cacgcgttat tcatgtcgtc gataaaaaaa
     8401 gcggcaaagt ggatggtgcc tgggagctgt cagataatct taaagagctg cgtttacgcc
     8461 acctcgaacc gaaacgtgat ttgatcgtta ctattggcaa ggaggtcaaa gcactcaaca
     8521 acgcaacctt cagtaaagat tacgaaaaaa ctataactac ccgcgacatc caacccagcg
     8581 tcggttttgc cagccgtggt tcgctgctgc ctggtaaagt cgttgaaggg ctgccggtaa
     8641 tggcgctcaa cgttaataat gtcgatgtta acttctttcg cgttaagcca gaatctctgc
     8701 cagcattcat tagccaatgg gaataccgca attcgctggc gaactggcag tcagacaaac
     8761 tgctgcagat ggcggatctg gtctacaccg gacggtttga tctcaatcct gcgcgtaaca
     8821 cccgtgaaaa attattgctg ccgctgggcg atatcaaacc gcttcagcag gcgggcgtgt
     8881 atctggctgt gatgaatcag gctggacgtt acgattacag taatcccgcg acgctgttta
     8941 cgttaagtga tatcggcgtt tcagctcacc gttatcacaa tcgtcttgat atctttaccc
     9001 aaagtctgga aaacggcgcg gcccagcaag gaattgaagt ctctttatta aatgagaaag
     9061 ggcagactct gactcaggca accagtgacg ctcaggggca tgtgcagctg gaaaatgata
     9121 aaaacgcggc attactgttg gcgcgtaaag acggtcagac aacgctactc gatttaaaac
     9181 ttccggcgct ggacttagca gaatttaaca ttgctggcgc gccaggctat agcaaacagt
     9241 ttttcatgtt tggcccgcgc gatctttatc gcccgggtga aacggtaatc ctcaatggtt
     9301 tgctgcgtga tgcagacggt aaagcgttgc ccgatcaacc catcaagtta gacgtgatta
     9361 aacccgacgg gcaggttctc aggagcgtcg ttagtcagcc ggagaatggc ctctaccact
     9421 taacctggcc actcgatagc aatgcggcaa ccggtatgtg gcatattcgc gctaacacgg
     9481 gcgataacca gtatcggatg tgggatttcc acgtcgaaga ttttatgcca gagcgtatgg
     9541 cgctgaatct gaccggtgag aaaaccccgc ttacgccgaa tgatgaagtg aaattctccg
     9601 tggtgggata ctacctgtac ggtgcgcctg ctaatggtaa tactttgcaa gggcaacttt
     9661 tcctgcgccc actgcgtgag gctgtgtcag ccttacctgg ttttgaattc ggcgatatag
     9721 ctgccgaaaa cctttcccgc acgctggatg aagttcagtt gacgctggat gataaagggc
     9781 gcggcgaagt ttctacagaa agccagtgga aggaaacgca ttccccatta caggttattt
     9841 tccagggtag tttgctggaa tcgggcggtc gcccggtgac gcgccgcgct gagcaagcta
     9901 tctggcctgc cgatgcattg ccggggatcc gtccgcagtt cgcctcgaaa tcagtttacg
     9961 attatcgtac tgacagcacg gtgaaacagc ccattgttga tgaaggcagt aacgccgctt
    10021 ttgacatcgt ttatagcgat gcgcaaggcg tgaaaaaagc cgtgtcgggc ttgcaggtgc
    10081 gcctgattcg cgaacgccgc gattactact ggaactggtc agaagatgaa ggctggcagt
    10141 cacagtttga tcaaaaagat ctgatcgaaa atgaacaaac tctggatctg aaagcggacg
    10201 aaaccggcaa ggtcagtttt ccggtagagt ggggtgctta tcgtctggaa gtcaaagcgc
    10261 cgaatgaagc ggtcagtagt gttcgtttct gggctggcta tagctggcag gacaacagcg
    10321 acgggagcgg tgccgtgcga cccgaccgtg tcacgctgaa actggataaa gccagttatc
    10381 gccctggcga taccattaag ttgcatattg ccgcgccaac ggcgggtaaa ggttatgcga
    10441 tggtcgagtc cagtgaaggg ccgctgtggt ggcaagagat tgatgttccg gctcaagggc
    10501 tggatctgac gattccggtc gataaaacct ggaatcgtca tgatctttat ttgagtacgc
    10561 tggtggtgcg tcctggcgat aaatctcgct ccgcgacgcc aaaacgcgcg gttggtgtgt
    10621 tgcatctgcc gcttggtgat gaaaaccgtc gcctcgatct ggcgctggaa acaccagcaa
    10681 aaatgcgtcc caatcaacca ttaaccgtga aaattaaagc cagcactaaa aatggcgaga
    10741 agcctaaaca ggtgaatgtg ctggtgtctg ccgttgatag tggtgtgctg aatattactg
    10801 actacgtcac gccagatccg tggcaggcgt tctttggtca gaaacgctat ggcgcagaca
    10861 tttacgatat ttacggtcag gttattgaag gtcaggggcg tctggcagct ctgcgtttcg
    10921 gtggcgatgg tgatgagctg aaacgtggtg gtaaaccgcc ggtcaatcac gtcaatattg
    10981 tcgcgcagca ggcgctgccg gtaacgctca acgaacaggg cgaaggctcg gttacactgc
    11041 cgattggcga ttttaacggt gaattgcgcg tcatggcgca agcctggacg gcagatgact
    11101 tcggtagcaa cgaaagtaaa gtgatagttg ccgcaccggt gattgctgaa ctgaacatgc
    11161 cgcgctttat ggcgagtggc gatacctcgc gtctgacgct ggatatcact aatcttaccg
    11221 ataaaccgca aaaactgaac gttgccctga ccgccagtgg tttgcttgaa ctggtcagcg
    11281 attcacccgc agccgttgaa ttagcgccag gtgtgcgtac tacgctgttt atcccggtgc
    11341 gagcattgcc gggttatggc gatggagaaa ttcaggccac cattagcggg ttagcgttac
    11401 cgggtgaaac cgttgccgat cagcataagc agtggaaaat cggcgtccgt ccggcgttcc
    11461 cggcacaaac ggttaattac ggtacggcgt tacagcctgg tgagacatgg gcgattccgg
    11521 cggatggatt gcaaaacttc tcgcctgtta cgctggaagg gcaattgttg ttgagcggca
    11581 aaccaccgct gaacatcgca cgttatatca aagagttaaa agcgtatccg tacggctgtc
    11641 ttgagcaaac cgccagcggc ctgtttccgt cactttatac caacgcagcc caactgcagg
    11701 cgttgggcat caaaggcgac agtgatgaga aacgccgtgc atcggtcgat atcggcattt
    11761 cccgtttgct gcaaatgcaa cgtgataacg gcggctttgc gctgtgggat aaaaacggtg
    11821 acgaagagta ctggctgacg gcttacgtga tgg
//
LOCUS       DACAPB010000143        11760 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.143, whole genome shotgun sequence.
ACCESSION   DACAPB010000143 DACAPB010000000
VERSION     DACAPB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.143"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1200)
                     /gene="ravA"
                     /locus_tag="IT904_003446"
     CDS             complement(<1..1200)
                     /gene="ravA"
                     /locus_tag="IT904_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAP3193185.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGK"
     gene            1423..3291
                     /gene="kup"
                     /locus_tag="IT904_003447"
     CDS             1423..3291
                     /gene="kup"
                     /locus_tag="IT904_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAP3193186.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            3458..3877
                     /gene="rbsD"
                     /locus_tag="IT904_003448"
     CDS             3458..3877
                     /gene="rbsD"
                     /locus_tag="IT904_003448"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP3193187.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            3885..5390
                     /gene="rbsA"
                     /locus_tag="IT904_003449"
     CDS             3885..5390
                     /gene="rbsA"
                     /locus_tag="IT904_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAP3193188.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            5395..6360
                     /gene="rbsC"
                     /locus_tag="IT904_003450"
     CDS             5395..6360
                     /gene="rbsC"
                     /locus_tag="IT904_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAP3193189.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            6385..7275
                     /gene="rbsB"
                     /locus_tag="IT904_003451"
     CDS             6385..7275
                     /gene="rbsB"
                     /locus_tag="IT904_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HAP3193190.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            7401..8330
                     /gene="rbsK"
                     /locus_tag="IT904_003452"
     CDS             7401..8330
                     /gene="rbsK"
                     /locus_tag="IT904_003452"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP3193191.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            8334..9326
                     /gene="rbsR"
                     /locus_tag="IT904_003453"
     CDS             8334..9326
                     /gene="rbsR"
                     /locus_tag="IT904_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAP3193192.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(9292..10719)
                     /locus_tag="IT904_003454"
     CDS             complement(9292..10719)
                     /locus_tag="IT904_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP3193193.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAISAAVLRVYEGTEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(10742..11434)
                     /locus_tag="IT904_003455"
     CDS             complement(10742..11434)
                     /locus_tag="IT904_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP3193194.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGKYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDT"
BASE COUNT         2888 a         2843 c         3082 g         2947 t
ORIGIN      
        1 tttgccgcga atttcaccac ctttactcag ccactgttcc agcgcgttac gctcaaagga
       61 gatatgaacc acttccatat catgcaattt taacggtttt tgcagcagca gagtcagagt
      121 ggaagcagta acgttaacag gaagttgata ctgctgacga cggctgaaaa tgccgcccag
      181 acgaattacc gttaaggctg ttttatcgct ttgttgctgc tggagttgca ggtgacgttg
      241 cacaatcgcg ccaaggcgag tcaacatccc ttgctgttgc caggcgtgac cggtcatcaa
      301 tacatcaatt tgttgttgta tcaaattcag gctttgcgcg tcataccaca ggcaatcttt
      361 cagcaaaatg agatctaccg gggcaacagc actgcgaccg ctaaaaaagg cgctggcctg
      421 taataatcgg atcgcttttt tccagcgacg atccgagaca taaggcgcat ccggtaattt
      481 atccagttgc tggcgcagca taaaaatcag ctcaaataca tgatcgggca gcgtaatttc
      541 accaatctct ttctgccagc gttcatattc ttcatctgtg acctgcaagg catcaggaac
      601 cggattgtcg ttttcatcct gttgactggt cagcatggag cggaaattcg ctttatcctg
      661 cactttatct aaccacagac gaatcagcat gcggtcatat aacgcttcca gactgctgtc
      721 tgcttccggc agctcgttgg aggccgccac cagcagacgc atcgggattt tttctacgtg
      781 tgcaccgttg cggaactggc gctcgttaat ggcggtgagc aaggtattaa gaattgccgg
      841 gcccgctttc cagatctcat ccagaaagac aatttctgct tccggcaggt aaccgctggt
      901 taaacgttca tagcgccctt catcttttag cgcctgaata gaaaggggac caaaaacttc
      961 ttccggcgtg gagaagcggg tcatcagata ttcaaacgcg cgggcattct gaaaggcgaa
     1021 ttttaagcgc cgggcgatca aacttttggc aatacctggc gggccaagga ggaacacact
     1081 ttcaccactt aatgccgcta ataaacacaa gcggatggcg tggctacgtt cataaagccc
     1141 cttttccaac gaactgctca ggcgggaaat tctttccgct aataaatgag ggtgagccat
     1201 aatgaagtgg cgtcctttcg tcaaaagttc tgcgtaaatt gcgagtatag acgtttcttg
     1261 ctggtggcta aaatagtctc aaaggggggg tatttttctt tgagccaggt taatgtggcc
     1321 gcatttagga gtacgatttt gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa
     1381 gggactaagc acacatttca tatttcaacg aaagactagt ctatgagcac tgataataag
     1441 caatcattgc ccgcgattac cctcgcggcg attggagttg tctacggcga tattggtacc
     1501 agcccgttat atacacttcg tgaatgtttg tccggccagt ttggttttgg cgttgaacgc
     1561 gatgccgtgt ttggcttttt atcgctgatc ttctggctgc taatctttgt ggtttccatt
     1621 aaatatctca ccttcgtgat gcgggcagat aacgccggtg agggggggat cctgacgttg
     1681 atgtcgcttg ccgggcgtaa tacgtcggcg cgaaccacat caatgctggt gattatgggg
     1741 ctaatcggcg gcagcttttt ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg
     1801 tcagccattg aaggtctgga aatcgtcgcc ccgcagctgg atacctggat agttcccctc
     1861 tcaattatcg ttctcacttt attatttatg attcaaaaac atggcacggc tatggtcggt
     1921 aagctgtttg cgccgatcat gctgacctgg tttttgattc tggcaggact ggggttacgt
     1981 agcattattg ctaacccgga agtgctgcat gcgctgaatc caatgtgggc ggtgcatttc
     2041 ttccttgaat acaaaacggt ttcttttatt gcattagggg cagtggtgtt gtcgattacg
     2101 ggggtcgagg cgctgtatgc tgatatgggg cactttggta agttccctat tcgcctggcg
     2161 tggttcaccg tcgtattacc ttccttgacc cttaattact tcggtcaggg agcgctgttg
     2221 ttaaagaacc cggaagcgat taagaacccg ttcttcctgt tggcaccgga ctgggcgctg
     2281 atcccgctgc tgatcatcgc cgcactggcg acggtaattg cctcgcaggc ggttatctct
     2341 ggcgtcttct cattgacgcg tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt
     2401 attcacacct ccgaaatgga gtcagggcaa atctatattc cgtttgtgaa ctggatgctc
     2461 tatgtcgcgg tcgtgattgt gattgtcagc tttgagcact ccagcaacct ggcggcggcg
     2521 tacgggattg cggtgaccgg aaccatggtg ctgacgtcta ttctctcgac taccgtggca
     2581 cgtcagaact ggcactggaa taagtatttt gttgcgctga tcctgattgc tttcctttgt
     2641 gtcgatattc cattgttcac cgctaacctc gataaactgc tctccggcgg ctggttgcca
     2701 ttgagcctcg gtactgtgat gtttatcgtg atgaccacct ggaagagcga gcgtttccgc
     2761 ttgctgcggc ggatgcatga acatggtaac tctctggaag cgatgattgc ttcgctggag
     2821 aaatcaccgc ctgttcgcgt gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc
     2881 attccctttg cgctgatgca taaccttaaa cataacaagg tattgcatga gcgggtgatt
     2941 ctgttaactc tgcgtaccga agacgcgcca tatgtccata atgtccgtcg ggtacagatt
     3001 gaacaactgt cgcccacttt ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca
     3061 aacgtggaag aagttttcca ccgctgcggt ctggaaggat taagttgccg gatgatggaa
     3121 acctccttct ttatgtcgca tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt
     3181 ctgcgcggca agctgtactt gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt
     3241 gaaattccgc caaacagggt tatcgaactg ggtactcagg tcgaaatcta acgccagacg
     3301 cctcctttct ccataagggg gcgtttttgt tttcatggtt aatcaccatg taaaacgttt
     3361 cgaggttgat cacatttccg taacgtcacg atggttttcc caactcagtc aggattaaac
     3421 tgcgggtcag cgaaacgttt cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa
     3481 ttctgatatt tcatcggtga tctcccgtct gggacatacc gatacgctgg tggtgtgtga
     3541 tgctggttta cccatcccca aaagtacaac gcgtatcgat atggcattaa cccagggtgt
     3601 accttctttt atgcaggtgc tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat
     3661 tatcgcggaa gagatcaaac aacataatcc gcaactccac gaaacgttgc tcactcacct
     3721 tgagcagctg caaaaacacc agggaaatac cattgaaatt cgttacacca cgcatgaaca
     3781 attcaaacaa caaaccgcag aaagtcaggc ggtaattcgc agcggagaat gttctccgta
     3841 tgcgaatatc attctctgtg ctggcgtgac gttctgaggc cgtcatggaa gcattacttc
     3901 agcttaaagg catcgataaa gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa
     3961 atgtctatcc gggccgcgtg atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca
     4021 tgatgaaagt gcttactggt atctatgctc gcgatgccgg cacgctttta tggctgggga
     4081 aagaaacgac atttaccggg ccgaaatctt cccaggaagc cgggattggg attatccatc
     4141 aggaactgaa cctgatcccg cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt
     4201 ttgttaatcg ctttggcaaa attgactgga aaaccatgta tgccgaagcg gataaattgc
     4261 tggctaaact taacctgcgc tttaaaagcg acaagctggt gggcgatctt tccatcggtg
     4321 accagcaaat ggttgaaatc gccaaagtgc tgagctttga gtcgaaagtc atcattatgg
     4381 atgaaccgac cgatgcgctg accgataccg aaaccgaatc cctgttccgc gtcatccgcg
     4441 agctgaaatc gcaaggccgc ggtattgtct atatctccca ccgcatgaaa gaaatcttcg
     4501 agatttgcga tgacgttacc gtttttcgtg atgggcaatt tattgctgag cgcgaagtgg
     4561 catcactgac cgaagattcg ctgattgaga tgatggtggg tcgcaagctg gaagatcaat
     4621 atccgcacct ggacaaagcg ccgggagata tccgcctgaa agtcgataat ctctgcggac
     4681 ctggcgttaa cgatgtctct tttactttac gcaaaggcga aattcttggc gtctctggtt
     4741 tgatgggcgc aggtcgtacc gaactgatga aagtgctcta cggcgcacta ccgcgcacca
     4801 gcggttacgt caccctggat gggcatgaag tcgttacccg ttcaccgcag gatggcttgg
     4861 caaacggcat tgtgtatatc tccgaagacc gtaaacgtga cggtttagtg ttgggcatgt
     4921 cagtaaaaga gaacatgtcg ctgacagcgc tgcgctactt cagccgcgct ggcggcagtt
     4981 tgaagcatgc cgatgaacag caggctgtga gtgatttcat tcgtctgttt aatgtgaaaa
     5041 ctccgtcgat ggaacaggca attggtctgc tttccggtgg caatcagcaa aaagtggcga
     5101 ttgcccgcgg tctgatgaca cgccccaaag tgttgatcct tgatgaacct acccgtggcg
     5161 tagatgtcgg cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct
     5221 tgagcatcat tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca
     5281 tcgtcatgca tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag
     5341 tgttaatggc tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca
     5401 acccagactg tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg
     5461 cttatcgctc tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc
     5521 atcaataact tattcaatat tctccagcaa acctcagtga acgccattat ggcggtcggg
     5581 atgacgctgg tgatcctgac gtcgggcatc gacttatcgg taggttctct gttggcgctg
     5641 accggcgcag ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgttgct
     5701 gctgctctcg cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt
     5761 cgcgtccagg cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg
     5821 gtttatacca acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc
     5881 tggtttggta ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc
     5941 ttcctcgcgg cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg
     6001 ggcggcaacg aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc
     6061 gtctattctc tttgtggtct gctggcatcg ctggccggga tcattgaagt ggcgcgtctc
     6121 tcctccgcac aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt
     6181 ctgggcggta cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca
     6241 ttaattcttg gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag
     6301 atgatcgtca aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa
     6361 taacgactac aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct
     6421 gttgcgctaa gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg
     6481 gtctccacgc ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg
     6541 gataaacttg gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg
     6601 gcgaacgtgc aggacttaac cgttcgcggc acaaaaattc tgctgattaa cccgaccgac
     6661 tccgacgcag tgggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact
     6721 cttgaccgcc aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg
     6781 ggcggcaaaa tcgctggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc
     6841 gagctgcaag gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag
     6901 gccgttgctg ctcacaagtt taatgttctt gccagccagc cagcagattt tgatcgcact
     6961 aaaggtttga acgtaatgca gaacctgttg accgctcatc cggatgttca ggctgtattc
     7021 gcgcagaatg atgaaatggc gctgggcgcg ctgcgcgcac tgcaaactgc cggtaaatcg
     7081 gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt gaatgatggc
     7141 aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg cgtcgaaacc
     7201 gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga tctgaaactg
     7261 gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa atacgtcatc
     7321 gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac acggtggcgc
     7381 attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt ggcagcatta
     7441 atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc gtaaccggta
     7501 accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta
     7561 gcggtgcgaa tatcgcgttt attgcctgta cgggtgatga cagcattggt gagagcgttc
     7621 gccagcagct cgccactgat aacattgata ttactccggt cagcgtgatc aaaggcgaat
     7681 caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg
     7741 ccggcgctaa tgctgccctt tccccggcgc tggtggaagc gcaacgtgag cgtattgcca
     7801 acgcgtcagc attattaatg cagctggaat caccactcga aagtgtgatg gcagcggcga
     7861 aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct cgcgaacttc
     7921 ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc
     7981 tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg
     8041 aaaaaggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg gctagcgtga
     8101 atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc attgctgccg
     8161 gagatacctt taacggtgcg ttaatcacgg cattgctgga agaaaaacca ttgccagagg
     8221 cgattcgttt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc gcacaacctt
     8281 ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga cgcttggcta
     8341 caatgaaaga tgttgcccgc ctggcgggcg tttctacctc aacagtttct cacgttatca
     8401 ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg attaaagaac
     8461 tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca cataccattg
     8521 gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt ggcgttgaac
     8581 gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc gatgaacagc
     8641 ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggcttg ctgttactgt
     8701 gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg
     8761 tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat aactcgttgc
     8821 tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt atcgcctgta
     8881 ttaccggccc gctggataaa actccggcgc gcctgcggtt ggaaggttat cgggcggcga
     8941 tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat tttgaattta
     9001 acggcgggtt tgacgctatg cgccaactgc tatcacatcc gctgcgtcct caggccgtct
     9061 ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag gcagagttac
     9121 aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca agctttatga
     9181 cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg gcgattgatg
     9241 tactcatcca tcggataacc cagccaactc ttcagcaaca acgattacaa cttactccga
     9301 ttctgatgga acgcggttcg gcttagattt acgctgtctt ttgatcaaat tattaccatc
     9361 ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc ccattgtgat
     9421 aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc gttccttcat aaacgcgaag
     9481 gacggccgca cttatagcaa cgcctaaact gatagacagt tgctgcgtga ccgccagaac
     9541 actgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg tattcatcgc
     9601 ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca gcatccatat
     9661 cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa tcaccgtgat
     9721 ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca tcgattttgc
     9781 aataatggaa cctaacgctg tcggcgccat catacagccg gcaataaacg cctgataacc
     9841 aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc ccagacgggt
     9901 tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat ctaatgaaat
     9961 taatgggttt ggcgtgcgtc gtgcatggag aatatagaga agcagtaacc cgatgctggt
    10021 gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata gctctattcc
    10081 gcttgagaag agaacaaggc tgaggccaaa caacaaaaag ccagtgatat cgaatctgcg
    10141 tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcctg caatacctat
    10201 ggggatattg attaaaaata tccagtgcca ggttgcccag gtgaccagca cgccgccaag
    10261 aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca atactggaag
    10321 aagttcatta cgaggataag cgcgcagtaa ggccagccga gcaacaggca tcatcattgc
    10381 gccgcctatc ccctgaataa cccggaagac aaccagctgt ggtagcgaat tagaaagtgc
    10441 gcaggccaga gaacccaatg tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc
    10501 gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca gcgtataact
    10561 gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga ttgcgggtaa
    10621 ggcggtattc agaatagtgg catcaagtgc ctgcatgaag aaggccatcg ccgcgatcca
    10681 cggcaaaccc gccatactgc gcttcttttt atcgctcatt caatgtcctg ttatcgggtt
    10741 gtcacgtatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc
    10801 tttggacaat cgcatcgaca atcgcctggt gcagatccag ctttatcact gtgtcgctgg
    10861 taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa
    10921 acggattggc gctcatttca tagatatgct catgccaggc catatctact tcgatccagc
    10981 gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta ttgagatgcg
    11041 ccttctgttc cgcggtgcca accgttgcag ccagtaggca agcttgcggc tccagacaga
    11101 tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc atccaccagg
    11161 taagcaattc ctgatcaaga aaattccagt ttgattgtgg catgacccgg gtaccaattc
    11221 gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg
    11281 tacgactcac tccaaattgc tcgcccagct caatctcacc aggcaaaatg gtgccgggtt
    11341 catatttacc ttttaagatg cgttgcgcca gcttctcagc cagaacatac gaaaggtttt
    11401 tctgtgcagc taactgttgt gcgcttaaag gcattactaa tcttcctttt tctttttatt
    11461 cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa attgcttgtt
    11521 ttatggcaca ttaacggggc ttttgctgaa aaaatgcgcg gtcagaaaat tattttaaat
    11581 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
    11641 caaacacgcc gccgggtcag agaagttctc ctgagaactt cggtagagaa agcaaaaata
    11701 aatgcttgac tctgtagcgg gaaggcgtat tatgcacacc ccgcgccgct gagaaaaagc
//
LOCUS       DACAPB010000144        11732 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.144, whole genome shotgun sequence.
ACCESSION   DACAPB010000144 DACAPB010000000
VERSION     DACAPB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.144"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..405)
                     /gene="ybcL"
                     /locus_tag="IT904_003456"
     CDS             complement(<1..405)
                     /gene="ybcL"
                     /locus_tag="IT904_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAP3193195.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDK"
     gene            complement(574..906)
                     /gene="emrE"
                     /locus_tag="IT904_003457"
     CDS             complement(574..906)
                     /gene="emrE"
                     /locus_tag="IT904_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAP3193196.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1250..1564)
                     /gene="fliE"
                     /locus_tag="IT904_003458"
     CDS             complement(1250..1564)
                     /gene="fliE"
                     /locus_tag="IT904_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAP3193197.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1779..3437
                     /gene="fliF"
                     /locus_tag="IT904_003459"
     CDS             1779..3437
                     /gene="fliF"
                     /locus_tag="IT904_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAP3193198.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDEGGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3430..4425
                     /gene="fliG"
                     /locus_tag="IT904_003460"
     CDS             3430..4425
                     /gene="fliG"
                     /locus_tag="IT904_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAP3193199.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4418..5104
                     /gene="fliH"
                     /locus_tag="IT904_003461"
     CDS             4418..5104
                     /gene="fliH"
                     /locus_tag="IT904_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAP3193200.1"
                     /translation="MSDNLPWKTWMPDDLAPPPAEFVPIVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQLLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5104..6477
                     /gene="fliI"
                     /locus_tag="IT904_003462"
     CDS             5104..6477
                     /gene="fliI"
                     /locus_tag="IT904_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAP3193201.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6496..6939
                     /gene="fliJ"
                     /locus_tag="IT904_003463"
     CDS             6496..6939
                     /gene="fliJ"
                     /locus_tag="IT904_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAP3193202.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6936..8063
                     /gene="fliK"
                     /locus_tag="IT904_003464"
     CDS             6936..8063
                     /gene="fliK"
                     /locus_tag="IT904_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAP3193203.1"
                     /translation="MIRLAPLITADVDTTTLSGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDEQSTSTPLNTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLIAEAQSKAEIISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8168..8632
                     /gene="fliL"
                     /locus_tag="IT904_003465"
     CDS             8168..8632
                     /gene="fliL"
                     /locus_tag="IT904_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAP3193204.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLITEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8637..9641
                     /gene="fliM"
                     /locus_tag="IT904_003466"
     CDS             8637..9641
                     /gene="fliM"
                     /locus_tag="IT904_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAP3193205.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9638..10051
                     /gene="fliN"
                     /locus_tag="IT904_003467"
     CDS             9638..10051
                     /gene="fliN"
                     /locus_tag="IT904_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAP3193206.1"
                     /translation="MSDMNNQADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10054..10419
                     /gene="fliO"
                     /locus_tag="IT904_003468"
     CDS             10054..10419
                     /gene="fliO"
                     /locus_tag="IT904_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAP3193207.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10419..11156
                     /gene="fliP"
                     /locus_tag="IT904_003469"
     CDS             10419..11156
                     /gene="fliP"
                     /locus_tag="IT904_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAP3193208.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11166..11435
                     /gene="fliQ"
                     /locus_tag="IT904_003470"
     CDS             11166..11435
                     /gene="fliQ"
                     /locus_tag="IT904_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAP3193209.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11443..>11732
                     /gene="fliR"
                     /locus_tag="IT904_003471"
     CDS             11443..>11732
                     /gene="fliR"
                     /locus_tag="IT904_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="FliR, with proteins FliP and FliQ, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAP3193210.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPIVSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRT"
BASE COUNT         2858 a         3116 c         3168 g         2590 t
ORIGIN      
        1 tttatctcct ttgggaggac atgctccacc aaatcctgca taaccaaaat catttcggcc
       61 ttgaacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag cgggcaaata
      121 tgttacagtt gctggaatat tagcaacagt ccagtgccac catccactgc ctgtaggagc
      181 atctggatca tacacagtta cggcaaagct tttggtgcct tcaggagcac cagaccaggt
      241 taatgagggc gatgtattac caccttcaca cccaaatcca gaaaatacat gagacgttgt
      301 aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg cctgcgcaga
      361 aaatgttatg aatgccaata cacttgaaac gataagtttt ttcataaaaa cctcattgtt
      421 gtgacctatc gttattttat ttgatgtgcc ttgatctcat tatgcataaa ggcgcaatga
      481 tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa tatgcacaaa
      541 tcagattcct gttatctcaa ggataattat tttttaatgc ggtgcacttc gtgacaacaa
      601 gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg gcaggtccag
      661 tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga cgcctgacca
      721 gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata accagaatga
      781 tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc cctctgaaaa
      841 cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat aaatataagg
      901 gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt ttgaacacaa
      961 ctcaccgttt tcatcctagt gtatgtgtat ccagggggct ctggcgtggc aagagataaa
     1021 ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca ctcttcctgg
     1081 gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc agaaaacttt
     1141 caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc catgccccac
     1201 gctgtgggat cggatataat aaacaagcat agtcaacagg ttacaaaacc tacacctgca
     1261 tggacatcac ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca
     1321 tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
     1381 cgagggtgaa tttttctgcc tgcgtgcggg cagctgtttg tgtatcgcta atgcgatcga
     1441 gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgaa tcctgcgcac
     1501 gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
     1561 acattttcgt ctcctggata atttctggta gcaaagccta ccagtaagtc aataagacaa
     1621 aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
     1681 aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
     1741 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
     1801 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
     1861 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     1921 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     1981 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gttccggcag
     2041 ataaagttca cgagctgcgt ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg
     2101 tcggtttcga actactcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
     2161 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
     2221 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
     2281 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
     2341 ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca
     2401 cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg cgcgatctta
     2461 atgatgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag
     2521 caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg gcgcagctgg
     2581 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg
     2641 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
     2701 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
     2761 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc
     2821 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
     2881 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
     2941 tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt gaagatctga
     3001 cccgcgaggc gatgggcttt tctgaaaaac gcggcgactc gctcaatgtc gttaactcgc
     3061 cgttcaatag cagtgacgaa ggcggcggag aactgccgtt ctggcaacag caagcgttta
     3121 tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt
     3181 ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa gctgtacagc
     3241 aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
     3301 aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg agccagcgta
     3361 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
     3421 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt
     3481 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg
     3541 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg
     3601 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
     3661 cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt
     3721 ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagt
     3781 gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg
     3841 aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
     3901 atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa
     3961 gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga
     4021 acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc
     4081 gtgcgtgaat tcgacggcga actggcgcaa aaaattatcg acgagatgtt cctgttcgag
     4141 aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa
     4201 tcgttgctga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
     4261 atgtcgcaac gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt
     4321 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg tcttgccgaa
     4381 accggcgaga tggtgattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
     4441 aacctggatg ccggacgatc ttgcgccacc accggcagag tttgtgccaa tagtcgaacc
     4501 ggaagaaacc atcattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca
     4561 aatgcaggcc cacgagcagg gttatcaggc ggggattgcc gaaggtcgcc agcaaggtca
     4621 cgaacagggc tatcaggaag gactggccca ggggctggaa caaggtctgg cagaggcgaa
     4681 ggctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
     4741 ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcagc
     4801 acgtcaggtg atcggtcaga cgccaacggt ggataactcg gcactgatca aacagatcca
     4861 actgttgttg cagcaagaac cgttgttcag cggcaaaccg cagctgcgcg tgcacccgga
     4921 tgatctgcaa cgtgtggatg atatgctcgg cgcaacttta agtttgcatg gctggcgctt
     4981 gcggggcgat cccaccctcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct
     5041 cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt
     5101 gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataacttcga agctaaaatg
     5161 gcgcagttgc ctgcggtacg tcgctacggg cgattaactc gcgctaccgg gctggtgctg
     5221 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tgattgagcg ccagaacggc
     5281 agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
     5341 atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt
     5401 tcggctgaag ggctgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgc
     5461 gttctggacg gcagcggtaa accgctcgat ggcctcccct cccccgatac gacggaaacc
     5521 ggtgcgctga ttaccccgcc atttaaccca ttgcaacgta caccgattga acatgtgctg
     5581 gacaccggcg tgcgcccaat caatgccctg cttaccgtcg ggcgcgggca gcgtatgggg
     5641 ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc ccgttacacc
     5701 cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgcg gacgcgaagt aaaagatttt
     5761 attgagaata tcctcggtgc cgaagggcgt gcacgctccg tggtgattgc cgctccggcg
     5821 gatgtttctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgaat tgccgaagat
     5881 tttcgcgatc gtggtcagca tgtattgctg attatggact ccctcacccg ctacgcgatg
     5941 gcccagcgtg agattgcgct ggcgattggc gaaccacctg ccactaaagg ttatccaccg
     6001 tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc
     6061 ggctcgatta ccgcgtttta taccgtgctc accgaaggcg atgaccagca ggaccccatt
     6121 gccgactccg cgcgggccat cctcgacggc cacattgtgc tgtctcgccg actggcggaa
     6181 gccgggcact atccggctat cgatattgaa gcgtcgatca gtcgcgcaat gacggcgttg
     6241 atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag
     6301 cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgat
     6361 aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat ttttgaacgc
     6421 gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc
     6481 aggagataac ggcagatggc ggaacatggt gcgctggcga ccctgaaaga tctggcagaa
     6541 aaagaggtag aggatgccgc tcgcctgctg ggtgaaatgc gtcgcggatg tcagcaggcg
     6601 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaatagc
     6661 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
     6721 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
     6781 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
     6841 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
     6901 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttgatta
     6961 ccgccgacgt tgacaccacc acattgtctg gcggcaaagc cagcgatgct gcacaagatt
     7021 ttctcgcgtt gttgagcgaa gcattaacag gcgaggcaac aaccgacaaa gcgactcccc
     7081 agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctggtc agcgatattg
     7141 tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacaccg cctgtcatca
     7201 acgacgaaca atccacatca acaccgttaa acaccgctca gacgataacg ttggctgcgg
     7261 cggctgacaa caatacggca aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg
     7321 ccagcctgag tgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
     7381 atgcaccgtc aaccgtatta ccgacagaga aaccaacgct cttcacaaaa ctgacttctg
     7441 cgcaactcac aacagcacag cctgatgacg cccccggcac gccagctcag ccattaacac
     7501 cgctgatagc agaagcccag agtaaagcgg aaatcatcag cacgccttcg ccggtgaccg
     7561 ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc
     7621 ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
     7681 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcacccg caggatttag
     7741 gtgaggtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag atggtttcac
     7801 cgcatcagca tgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg
     7861 ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc
     7921 agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa cctctggcgg
     7981 gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta acaggcaaca
     8041 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
     8101 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
     8161 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
     8221 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
     8281 tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc
     8341 gctggatacc tttacggtca atctgggcga tgcggatcgc gtgctgtata tcggcataac
     8401 cctgcgcctg aaagatgaag ctacacgctc gcggctgagt gagtatttgc cggaagtccg
     8461 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
     8521 caagaaaaac ctgattaccg agattaaaac cacgctttcc accccgcttg ttgccgggca
     8581 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
     8641 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
     8701 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
     8761 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
     8821 cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg tagcccagat ataaccgtcg
     8881 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
     8941 tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt tcaccgagtc
     9001 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
     9061 aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc
     9121 ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt
     9181 cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
     9241 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt ctgccattca
     9301 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
     9361 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg
     9421 tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg
     9481 acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca tgttgacggc gtcccggtgc
     9541 tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa catttgatta
     9601 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatcaggc
     9661 tgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc
     9721 aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
     9781 cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga ccgtcgagct
     9841 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt ccgtcgtggc
     9901 gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt taatcgccca
     9961 gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata tcattactcc
    10021 gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct
    10081 tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat cgccattatt
    10141 gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc gaaacgcact
    10201 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg
    10261 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    10321 ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga ttttcagtcg
    10381 gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg ttgtctgtcg
    10441 cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg ggtatcacca
    10501 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    10561 tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca
    10621 tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg
    10681 tattgctggg gctggcacta tttttgacct tttttattat gtcaccggtg atcgacaaaa
    10741 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag gaggcgctgg
    10801 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag gcggatttag
    10861 ggttgtttgc cagactggcg aatactggtc cgttgcaggg acctgaagcc gtgccgatgc
    10921 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    10981 cgattttcat cccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    11041 ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac
    11101 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    11161 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    11221 cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc agtattttgc
    11281 aggccgccac ccagatcaac gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat
    11341 ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    11401 ccttgttcac taacctgccg tatatcatcg ggtagccgta ctatgttgca ggtgacaagc
    11461 gaacaatggc tatcctggtt aagcctctac ttctggccgt tactgcgcgt gctggcgctc
    11521 atttctaccg cgccaattgt gagcgaacgc agcgtgccga aacgggtaaa actgggtctg
    11581 gcaatgatga tcacgttcgc aattgcccca tcattacctg ccaacgatgt tcctgttttt
    11641 tcgttctttg ctctgtggct ggccgtgcag cagatcctga tcggcattgc gcttggtttt
    11701 accatgcaat ttgcctttgc cgctgtgcga ac
//
LOCUS       DACAPB010000145        11659 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.145, whole genome shotgun sequence.
ACCESSION   DACAPB010000145 DACAPB010000000
VERSION     DACAPB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.145"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(40..1398)
                     /locus_tag="IT904_003472"
     CDS             complement(40..1398)
                     /locus_tag="IT904_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP3193211.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEER
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1985..4408
                     /gene="pgaA"
                     /locus_tag="IT904_003473"
     CDS             1985..4408
                     /gene="pgaA"
                     /locus_tag="IT904_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HAP3193212.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKVLSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4417..6435
                     /gene="pgaB"
                     /locus_tag="IT904_003474"
     CDS             4417..6435
                     /gene="pgaB"
                     /locus_tag="IT904_003474"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAP3193213.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREARRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            6428..7753
                     /gene="pgaC"
                     /locus_tag="IT904_003475"
     CDS             6428..7753
                     /gene="pgaC"
                     /locus_tag="IT904_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAP3193214.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            7755..8168
                     /gene="pgaD"
                     /locus_tag="IT904_003476"
     CDS             7755..8168
                     /gene="pgaD"
                     /locus_tag="IT904_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAP3193215.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(8218..9282)
                     /gene="phoH"
                     /locus_tag="IT904_003477"
     CDS             complement(8218..9282)
                     /gene="phoH"
                     /locus_tag="IT904_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAP3193216.1"
                     /translation="MVTSCTGHVLDNQRATTRGVFSSGSHLVTLHFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMGGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(9627..10898)
                     /gene="efeB"
                     /locus_tag="IT904_003478"
     CDS             complement(9627..10898)
                     /gene="efeB"
                     /locus_tag="IT904_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAP3193217.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYFGSALLRV"
     gene            complement(10904..>11659)
                     /gene="efeO"
                     /locus_tag="IT904_003479"
     CDS             complement(10904..>11659)
                     /gene="efeO"
                     /locus_tag="IT904_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAP3193218.1"
                     /translation="QSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDAIKAGDIEK
                     AKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHRLEKALFGD
                     NTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKISGEEDRYS
                     HTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKYRTKDGFET
                     YDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
BASE COUNT         3162 a         2773 c         2621 g         3103 t
ORIGIN      
        1 cctgtgtcca tattacgtgg gtaggatcaa aatgccgctt tatggtgact cacaaaaatt
       61 caccacatcc ctcacaacca ccttattacg cccggtctct ttcgcctcgt aaagtgcgtt
      121 atcagccagt cgatagatct catttgggtt taacgcattt tcctgagtca caacagcgcc
      181 aatactaatc gtcacttttt gtggtatagc gtattcagga ttatcgccag ttaaacgctc
      241 aacattttcc cgaatccttt ccgctaaagc tttcgcacgt tcagtatcga tgtccgtcag
      301 caagacgcca aactcttcgc cgccgacgcg cgctaaaagg tcatctggtc gtatgctttt
      361 cccaatgata ttcaccactg tttttatcac ctgatcgcca accggatgcc cccaggtgtc
      421 gttgactttt ttgaagtggt cgatatccat aatcatgacg caataaggcg ttttttgggc
      481 tgatgctgat tgaactgtca gttcattaaa aaaataattt ctgttaaata tattggttaa
      541 gggatcgcga tgtgcaatgt tctttgttac tcgtagcgca ctgaatatat ggcacataaa
      601 tattaccata acggttaact tactgactac ttcaatagtg cgactaatat accaagtgga
      661 tacgctatac ctgctcataa ataacagaga gatattgtac agtacggcac aaaaagcaat
      721 caccgtcact ccattccaga tatcatagcg taaaccatta aagtatagat taaccgaaag
      781 caaggttaac cacatgaaaa tcattatggt aacatatgag gctttccaaa ccacctgacc
      841 attttcattg gttaactcgg caatgtgtaa attgtagctc tcataatgac tagaaagtat
      901 gtgagcaact atcgcccccc caaaaacaaa aaacaaactt atgcacaaag tcattttttt
      961 ggaaaataaa ttgcgctgtt ttgtgttttc actaacttta ttcaccaatg ccagaaatat
     1021 taatatgcac aaactcatct gacgaaacaa ataatagatt gcaatgtcat tttggacaac
     1081 actgcttgtc aaacgctcct caataatttg ctgaatgata atgacggttt taacaaagta
     1141 aatcaaacaa ctcagaaaag ccaaactaag tacacagttt gaaaattgcc tacggtcata
     1201 aatgaaatac aactgcataa aaataaatgc aacagtatcg ataaaaaata aagcaatcat
     1261 taccatcaga taggttgatc gtggaacaac ttcctcaacg cctggctgat taaaccaact
     1321 attcaccaaa accaaagcaa gcccaaacaa taagctcact aatacagtac tactaattct
     1381 caaatagtct ttttccataa agctacacca atctgtaggt tgtagatccc tttctattat
     1441 taatagatat aagaactgta ctgttgtcaa tatgaaattc cgcccacaaa caataccgat
     1501 gatgatagtt gctgaatgta attattattg cctaagtgct ttatacattg tttgcaacaa
     1561 gccaggcttg aaaatactta taaatggtta tagaaaatcc ttataaaaca ttaattcata
     1621 gatacattag gaataacaat taaatccgtg agtgccgtag cgcagccttt catcaggact
     1681 ttcgaaaaat ccgaaatcat gcatcggaat ttactgattt aattatttta atcctaattt
     1741 attttgaaaa aggcattggg atttatgccg tattcctgaa gatcctcatc attggaatgg
     1801 attttcgggc gagaaaagga ttttatatgg acactctgct catcatttct tcttctcatc
     1861 atcaacaatt cacgtctctc ttccgcgttt aataacggat tatgaggtgc aaaaatatct
     1921 ttcttttcag ttacctgtaa ttagatacag agagagattt tggcaataca tggagtaata
     1981 caggatgtat tcaagtagca gaaaaaggtg cccgaaaacc aaatgggctt tgaaacttct
     2041 tactgccgca tttttagcag cgagtcccgc ggcgaagagt gctgttaata acgcctatga
     2101 tgcattgatt attgaagctc gcaagggtaa tactcagcca gctttgtcat ggtttgcact
     2161 aaaatcagca ctcagcaata accaaattgc tgactggtta cagattgcct tatgggccgg
     2221 gcaagataaa caggttatta ccgtttacaa ccgctaccgt catcagcaat taccagcgcg
     2281 tggttatgca gctgtcgccg tcgcttatcg taacctgcaa caatggcaaa actcgcttac
     2341 actgtggcaa aaggtgctct ctctggagcc gcaaaataag gattatcaac ggggacaaat
     2401 tttaaccctg gcagatgctg gtcactatga tactgcgctg gttaaactta agcagcttaa
     2461 ctctggagca ccggacaaag ccaatttact cgcagaagcc tatatctata aactggcggg
     2521 gcgtcatcag gatgaattac gggcgatgac agagtcatta cctgaaaatg catctacgca
     2581 acaatatccc acagaatacg tgcaggcatt acgtaataat caacttgctg ccgcgattga
     2641 cgatgccaat ttaacgccag atattcgcgc tgatattcat gccgaactgg tcagactgtc
     2701 gtttatgcct acgcgcagtg aaagtgaacg ttatgccatt gccgatcgcg ccctcgccca
     2761 atacgctgca ttagaaattc tgtggcacga taacccagac cgcactgccc agtaccagcg
     2821 tattcaggtt gatcatcttg gcgcgttatt aactcgcgat cgttataaag acgttatttc
     2881 tcactatcag cgattaaaaa agacggggca aattattccg ccctgggggc aatattgggt
     2941 tgcatcggct tatctcaaag atcatcagcc gaaaaaagca cagtcaataa tgaccgagct
     3001 cttttatcac aaggagacca ttgccccgga tttatccgat gaagaacttg cggatctctt
     3061 ttacagccac ctggagagtg aaaattatcc gggcgcgcta actgtcaccc aacataccat
     3121 taatacttcg ccgcctttcc ttcggttaat gggcacgcct acgagcatcc cgaatgatac
     3181 ctggttacag gggcattcgt ttctctcaac cgtagcaaaa tatagtaatg atcttcctca
     3241 ggctgaaatg acagccagag agcttgctta taacgcacca ggaaatcagg gactgcgcat
     3301 tgattacgcg agtgtgttac aagcccgcgg ttggcctcgt gcagcagaaa atgaattaaa
     3361 aaaagcagaa gtgatcgagc cacgtaatat taatctggag gttgaacaag cctggacagc
     3421 attaacgtta caagaatggc agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga
     3481 accgcaagat cccggcgttg tacgattaaa acgtgcggtt gatgtacata atcttgcaga
     3541 gcttcgtatc gctggctcaa caggaattga tgccgaaggc ccggatagtg gtaaacatga
     3601 tgtcgactta accaccatcg tttattcacc accgctgaag gataactggc gcggttttgc
     3661 tggattcggt tatgccgatg gacaatttag cgaaggaaaa gggattgttc gcgactggct
     3721 tgcgggtgtt gagtggcggt cacgtaatat ctggctcgag gcagagtacg ctgaacgcgt
     3781 tttcaatcat gagcataaac ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa
     3841 ctggcgtatt ggttcgcaac tggaacgcct ctctcaccgc gttccattac gggcaatgaa
     3901 aaatggtgtt acaggcaaca gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg
     3961 taagtacggt gtctcctggg ctttcactga tttttccgac agtaaccagc gtcatgaagt
     4021 ctcacttgag ggtcaggaac gcatctggtc ttcaccatat ttgattgtcg atttcctacc
     4081 cagtctgtat tacgaacaaa atacagaaca cgatacccca tactacaacc ctataaaaac
     4141 gttcgatatt gttccggcat ttgaggcaag ccatttgtta tggcgaagct atgaaaatag
     4201 ctgggagcaa atattcagcg caggtgttgg tgcctcctgg caaaaacatt atggcacgga
     4261 tgtcgtcacc caactcggct acgggcaacg cattagttgg aatgacgtga ttgatgctgg
     4321 cgcaacgcta cgctgggaaa aacgacctta tgacggtgac agagaacaca acttatacgt
     4381 tgaattcgat atgacattca gattttaagg ataaatatgt tacgtaatgg aaataaatat
     4441 ctcctgatgc tggtgagtat aattatgctc accgcgtgca ttagccagtc aagaacatca
     4501 tttataccgc cacaggatcg cgaatcttta ctcgccgagc aaccgtggcc gcataatggt
     4561 tttgtagcga tttcatggca taatgttgaa gacgaagctg ccgaccagcg ttttatgtca
     4621 gtgcggacat cagcactgcg tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg
     4681 gtcagtattg ctcaaattcg tgaagcacgt cgaggaggaa aaccgctacc ggaaaaagct
     4741 gtagtgctga cttttgatga cggctaccag agtttttata cccgcgtctt cccaattctt
     4801 caggccttcc agtggcctgc tgtatgggcc cccgtcggca gttgggtcga tacgccagcg
     4861 gataaacaag taaaatttgg cgatgagttg gtcgatcgag aatattttgc cacgtggcaa
     4921 caagtgcgag aagttgcgcg ttcccggctc gttgagctcg cttctcatac atggaattct
     4981 cactacggta ttcaggctaa tgccaccggc agcttattgc ctgtatatgt aaatcgtgca
     5041 tattttactg accacgcacg gtatgaaacc gcagcagaat accgggaaag aattcgtctg
     5101 gatgctgtaa aaatgacgga atacctgcgt acaaaggttg aggtaaatcc acacgttttt
     5161 gtttggcctt atggcgaagc gaatggcata gcgatagagg aattaaaaaa actcggttat
     5221 gacatgttct tcacccttga atcaggtttg gcaaatgcgt cgcaattgga ttccattccg
     5281 cgggtattaa tcgccaataa tccctcatta aaagagtttg cccagcaaat tattaccgta
     5341 caggaaaaat caccacaacg gataatgcat atcgatcttg attacgttta tgacgaaaac
     5401 ctccagcaaa tggatcgcaa tattgatgtg ctaattcagc gggtgaaaga tatgcaaata
     5461 tcaaccgtgt atttgcaggc atttgctgat cccgatggtg atgggctggt caaagaggtc
     5521 tggtttccaa atcgtttgct accaatgaaa gcagatattt ttagtcgggt tgcctggcaa
     5581 ttacgtaccc gctcaggtgt aaacatctat gcgtggatgc cggtattaag ctgggattta
     5641 gatcccacat taacgcgagt aaaatactta ccaacagggg agaaaaaagc acaaattcat
     5701 cctgaacaat atcaccgtct ctctcctttc gatgacagag tcagagcaca agttggcatg
     5761 ttatatgaag atcttgccgg acatgctgct tttgatggca tattgttcca cgatgatgct
     5821 ttgctttcag attatgaaga tgccagtgca ccggctatca cggcttatca gcaagcaggc
     5881 tttagcggga gtctgagcga aattcgacaa aacccggagc aatttaaaca gtgggcccgc
     5941 tttaaaagtc gtgcgttaac tgacttcact ttagaactta gtgcgcgcgt aaaagccatt
     6001 cgcggtccac atattaaaac tgcacgaaat atttttgcac ttccggtaat acaacctgaa
     6061 agtgaagcct ggtttgcaca gaattatgct gatttcctaa aaagctatga ctggaccgct
     6121 attatggcta tgccttatct ggaaggtgtc gcagaaaaat cggctgacca atggttaata
     6181 caattgacca atcaaattaa aaacatccct caggctaaag acaaatctat tttagaatta
     6241 caggcacaaa actggcagaa aaatggtcag catcaggcta tttcttcgca acaactcgct
     6301 cactggatga gcctattaca actgaatgga gtgaaaaact atggttatta tcccgacaat
     6361 tttctgcata accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat
     6421 ccgaaaaatg attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc
     6481 cctatgcgta gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc
     6541 gttttttatg tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg
     6601 ccactggccg tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc
     6661 cattatcatt ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt
     6721 agcacagcgt tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac
     6781 ccgtgccatc ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc
     6841 gcaaaaccag gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata
     6901 tctggtgtgc attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga
     6961 accgatgttg tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg
     7021 ttctaccctg gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa
     7081 gcgaacccag cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg
     7141 tcgcagcgcc ctggcagaag tgggttactg gagtgacgat atgatcaccg aagatattga
     7201 tattagctgg aagctgcagt tgaatcagtg gacgattttt tacgagccac gggcactgtg
     7261 ctggatatta atgcctgaaa cgttaaaagg gctgtggaaa cagcgcctgc gctgggctca
     7321 gggcggtgca gaagtattcc tcaaaaatat gacaaggttg tggcgcaaag aaaactttcg
     7381 aatgtggccg ctgttttttg aatactgcct gacgacaata tgggccttca cctgcctggt
     7441 cggtttcatt atttacgcag tccaacttgc cggtgtaccg ttaaatattg aattgacaca
     7501 tatcgctgcg acacatactg ccggaatatt attgtgtacg ttatgtttac tgcaatttat
     7561 tgtcagcctg atgatcgaga atcgctatga gcataatctg acttcatcgc ttttctggat
     7621 tatttggttc ccggttattt tctggatgct gagcctggca acgacattgg tatcatttac
     7681 acgagtcatg ttgatgccta aaaagcaacg cgcccgttgg gtaagtcccg atcgcgggat
     7741 tctgagaggt taatatgaac aatttaatta ttacgacccg acaatcacca gtacgtttac
     7801 tggttgatta tgttgccaca accatcttgt ggacattatt tgcgttgttc atattcttat
     7861 tcgccatgga tctgctgacg ggttattact ggcaaagcga ggccagaagc cgacttcagt
     7921 tctatttttt gctggcagtg gcgaatgccg tcgtgttaat tgtctgggcg ctgtacaata
     7981 agctgcgttt tcaaaaacag cagcatcatg cagcctacca atatacgccg caagaatatg
     8041 cagagagctt agcaatacct gatgagctct atcagcaact acaaaaaagc cacaggatga
     8101 gcgtacactt caccagccag gggcaaataa aaatggttgt ttcagaaaaa gcgctagtcc
     8161 gggcataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat aacacactta
     8221 actgtaggca tgcagcgtac gttggcaaag tgccgaacgt acgcagtcct ctttaccgaa
     8281 ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac ttaatccgga
     8341 gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa ccgtcacgtt
     8401 ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct gcgcctcgtc
     8461 aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg gcgcaatttc
     8521 caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta agcgccggac
     8581 cagcacgtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat ctccaggtaa
     8641 gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc tgtcgacatc
     8701 cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc cgcacccggc
     8761 ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt gcaattgcgc
     8821 ttcatttcgc gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac caatggcttc
     8881 tacgccgccc atctgcacaa gcgaggtgac cgattcttct tcacgctgct tatggctgcg
     8941 cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg ctttttgtct
     9001 tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg cagcgcgatt
     9061 atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt ttggataaaa
     9121 tgccgaaaaa cggctacgcg caccgtttac ggcgtcggta acacatgaaa agaaaggatg
     9181 aggttgaaaa tgcaaagtga cgagatgact accggaggag aaaactccgc gagtggtggc
     9241 gcgttgatta tctaaaacat gtccagtaca ggacgttacc atccgcgatc tccatagtga
     9301 ctgactatca ctgccgggaa cttccgctgc tacttaataa gtacaacaga tctcgcattt
     9361 attgcaacaa tatatttact tatatttaac tataaaacac catttcagtg acattagttt
     9421 ctactggaaa gatgacagag tgatgacagt gatgaaaaaa gctgtgtgct ttcagcagga
     9481 tttgcatcag gcatcagtgc acgatcgccg gatgcggcgt gaacgcctta tccagcctac
     9541 caaacccgca ctgattgtag gcatgataag acgcgcaagc gtcgcatcag gcatctgcgc
     9601 acaaatgcct tatccgccta aaaacattaa acccgcaata acgcgcttcc gaaataatcg
     9661 ttcgcgtcct tcacccccgg cagcgcaaaa aaataaccgc cgccgatagg tttaacgtat
     9721 tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg
     9781 tgttggtagc agacaaacag caaccccata tccagttgcc cggagttggt gacgcccagt
     9841 gaataactgt agccacgacg cagcatcagg ctggactcac tctccgccgt gcggggattc
     9901 gccagccgga tatggctgtc cagcgcgatc accttccctt ccgggtcgct ggcgtaatca
     9961 ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa
    10021 atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag
    10081 cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc
    10141 cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg
    10201 aaacccagca aattaatcgg cgtctctttg cctttactac gcgccgcgtg atcggaaata
    10261 aacccttccc gcttccagcg cacactgagc aaatccggcg tgtgtttgat gatatcgcgc
    10321 agcgcatgga taaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac atcaccatga
    10381 cataacgccg catccagcga gtcgttgggg aaacgcgtca tcttctgcag cttttttggc
    10441 atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg
    10501 gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc
    10561 ggatttggcg tttctggtgc tgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac
    10621 aagcgaaaca accgctcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc
    10681 atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt
    10741 ttctcattgc gagcatccgg tgaaagcgta cccggtgcac tttgcgtttt ttgtgcatga
    10801 gcgaccggac aacttcccgc cagcgccagt gcacctatca ctttcagtaa acgtcggcgt
    10861 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
    10921 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc agtgcattcc
    10981 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
    11041 gaatggtatc gaccttttta aagttggcat cgacttttgc cagcagttcc gggttggctt
    11101 tttgcagttg tggacgcagc aaatcgacaa ttttctgcga gccttcaacg ttagcctgga
    11161 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctaattttg ctggctgcca
    11221 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
    11281 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
    11341 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
    11401 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
    11461 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggt gcatacagtg
    11521 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
    11581 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
    11641 ttaacagcgc atcactttg
//
LOCUS       DACAPB010000146        11312 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.146, whole genome shotgun sequence.
ACCESSION   DACAPB010000146 DACAPB010000000
VERSION     DACAPB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.146"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..197)
                     /gene="moaA"
                     /locus_tag="IT904_003480"
     CDS             complement(<1..197)
                     /gene="moaA"
                     /locus_tag="IT904_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="together with moaC, is involved in the conversion
                     of a guanosine derivative (GXP) into molybdopterin
                     precursor Z; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAP3193219.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTE"
     regulatory      complement(185..329)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            594..1502
                     /gene="yvcK"
                     /locus_tag="IT904_003481"
     CDS             594..1502
                     /gene="yvcK"
                     /locus_tag="IT904_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAP3193220.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNVLEKALQALG"
     gene            complement(1694..3715)
                     /gene="uvrB"
                     /locus_tag="IT904_003482"
     CDS             complement(1694..3715)
                     /gene="uvrB"
                     /locus_tag="IT904_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAP3193221.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(4294..4971)
                     /gene="bioD"
                     /locus_tag="IT904_003483"
     CDS             complement(4294..4971)
                     /gene="bioD"
                     /locus_tag="IT904_003483"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP3193222.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(4964..5719)
                     /gene="bioC"
                     /locus_tag="IT904_003484"
     CDS             complement(4964..5719)
                     /gene="bioC"
                     /locus_tag="IT904_003484"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAP3193223.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(5706..6860)
                     /gene="bioF"
                     /locus_tag="IT904_003485"
     CDS             complement(5706..6860)
                     /gene="bioF"
                     /locus_tag="IT904_003485"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAP3193224.1"
                     /translation="MSWQDKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(6857..7897)
                     /gene="bioB"
                     /locus_tag="IT904_003486"
     CDS             complement(6857..7897)
                     /gene="bioB"
                     /locus_tag="IT904_003486"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAP3193225.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            7984..9273
                     /gene="bioA"
                     /locus_tag="IT904_003487"
     CDS             7984..9273
                     /gene="bioA"
                     /locus_tag="IT904_003487"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAP3193226.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            9332..9808
                     /locus_tag="IT904_003488"
     CDS             9332..9808
                     /locus_tag="IT904_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAP3193227.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            9960..11243
                     /locus_tag="IT904_003489"
     CDS             9960..11243
                     /locus_tag="IT904_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAP3193228.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
BASE COUNT         2555 a         3043 c         2957 g         2757 t
ORIGIN      
        1 ttttcggtgc ccagactggc gaaggcgcgc gtaacccggc gaatttcatc gacggtaaga
       61 aagcctttat tggtgacgcc gctcggtttg tagccatccg gcaggcagta ggtgcaacga
      121 aagttacaca catcggtaat cgacaggcgc aagtagtaaa acttacgcgc aaatgcatca
      181 gtcagttgtg aagccatgta cacctttcca gatacgggag gcgaagtcat ttcttccttc
      241 gccctggtgg caatctttac gagcattgtc acggccaaag caccgtatca gttgacccag
      301 gtgcagaggc tagagtgttt agtggttaag ccgatactag cgtgtaaatg agatttttac
      361 catccacact ttcgctatgt aatcatgtat atagcgtcat gatcgtgcta tttcgctaca
      421 gaataaggga aaatggcagt tttactttga tatacatcat cagggcggca tagcgtgcat
      481 cgtcctttag aggtagaaga aaatagtttg tatcctcaat attggcaggt taattgctgt
      541 ttccccgcaa tttgcgctac tgtagcgcgg ataatctgac tccaggaaac tatatgcgca
      601 atcgtacgct ggctgatctt gatcgtgtcg ttgctctcgg cggagggcat ggactgggac
      661 gcgttctctc atcactttcg tctttgggtt ctcgtttaac gggtatcgtc accaccaccg
      721 ataatggtgg ctcgacgggg cgtattcgcc gttcagaagg cggcattgcc tggggcgata
      781 tgcgcaactg cctcaatcag ctgataacgg aaccgagcgt cgcctccgcg atgtttgaat
      841 accgttttgg tggcaatggc gaactttccg gtcataatct cggaaacttg atgttaaagg
      901 cgctggatca ccttagcgtg cggcctctgg aagccatcaa tttaattcgt aatctgctga
      961 aagtggatac gcatttgatt ccaatgtcag agcatcctgt tgatctgatg gcgattgacg
     1021 atcaggggca tgaagtttac ggcgaggtca atatcgacca gttaactacg ccgattcaag
     1081 agttattgtt aacgcctaat gtacccgcaa cgcgtgaggc ggttcacgct atcaatgaag
     1141 cggatctcat cattattggg cctggcagtt tttataccag cctgatgcca attctgctgc
     1201 tgaaggaaat cgcccaggca ttacgccgca cgccagcgcc gatggtttat atcggcaatc
     1261 tggggcgtga gttgagttta cctgcggcta atttgaagct ggaaagcaag ctggcaatta
     1321 tggagcagta tgttggtaaa aaggtcattg atgcggtcat cgtcgggcca aaagtggatg
     1381 tctcggcggt gaaagagcgg attgtgatcc aggaggtact ggaggccagc gatattccgt
     1441 atcgtcatga ccgccagttg ttacataacg tgctggaaaa ggcgttacag gctttaggtt
     1501 aacggaaatc cgaaggaaaa ttccggcttc ctattgaaga caaagtgcgc gttgtttatg
     1561 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc gctaattcaa tgtattgcag
     1621 agatcatgta ggcctgataa gcgtagcaca tcaggcaatt ttgcttttgt catctgtctt
     1681 catcgttatc ctgtcacgac gccgcgataa acaattcacg cagctgatgc aactggtcac
     1741 gaatttgcgc cgcttcttcg aactccagat tctgcgcgtg ttgcatcatc aacccttcca
     1801 gttcatggat tttctgctgc agcgctttag gcgacatatc catcggcaca ttatccggct
     1861 caacaatcgg gcgcgatttt cctctgccct tcgctttggt tttggcaatg ttctgcccca
     1921 gcgccaggat atcgaccact ttcttgttca agccttgcgg cgtaatgccg tgttcttcgt
     1981 tgtacttctg ctgtttctcg cggcgacgtt cggtttcgcc aatcgctttc gccattgatg
     2041 gggtgatctt atcgccgtag agaatcgctt taccgttaac gttacgtgcc gcacgaccaa
     2101 tggtctggat caacgaacgt tcggaacgca ggaagccttc tttgtcagcg tcgaggatcg
     2161 ccaccagcga cacttccggc atatccagac cttcgcgcag taagttgatc cctaccagca
     2221 cgtcgaactc acccagacgc aagtcgcgga taatctccat acgttcgacg gtgtcgatat
     2281 ctgagtgaag ataacgcacg cgctcgccat gttcttcgag atattcggta agatcttccg
     2341 ccatccgctt ggtcagtgtg gtgaccagta cgcgttcgtt aattgccgct cgctgacgaa
     2401 tctccgaaag aagatcatca acctgtgtcg ccaccggccg cacttcgata atcgggtcaa
     2461 gcaatccggt tggacgcacc acctgatcca ccacatcgcc gccggatttt tccagctcgt
     2521 aattacccgg cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg aactcttcaa
     2581 acttaagcgg acggttatcc agcgctgatg gcaggcggaa gccgtactcc accagtgtct
     2641 ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg tggaatggtg acgtgagatt
     2701 catcgaccac cagcagccca tcagcaggga ggtaatcaaa cagcgtcggc ggtggctcac
     2761 ccggtccacg accggagagg aagcgcgagt agttttcaat ccccgaacag tagcccagct
     2821 cgttcatcat ctccagatca aactgggtac gctgggtcag ccgctgctct tccagcagtt
     2881 tgttgttttc caacagcact ttgcgtctgg cggccagctc ttctttgatc tcctccatcg
     2941 cctgtacgat gcgctcgcgc ggtgtgacgt agtgcgtttt cgggtagatg gtaaaacgtg
     3001 gaatagtgga aacaatctgc ccggtcagcg ggtcaaataa cgacaatcgt tccacttcct
     3061 cgtcaaacag ttccacgcga agtgcaatgt catccgattc tgccgggaag atatctatca
     3121 cctcgccacg aacgcggaaa gtaccacgct ggaatgcttg atcattacga gcgtattgca
     3181 gctccgccag tcggcgcaga atcgcgcgct gatcgataat cataccgacc gtgagatgga
     3241 gcatcatctt gagatataaa tcaggatcgc ccagaccata aatcgcggaa acagacgcca
     3301 ccacaaccac atcacgccgc tccagcatcg ctttggtggc ggataaacgc atctgctcaa
     3361 tatgttcgtt aaccgaggca tctttctcaa tgaaagtgtc ggaactcggt acataggctt
     3421 ccggctgata gtagtcgtag taggagacga aatactccac cgcgttttcc gggaagaact
     3481 ctttcatttc gccatacagc tgggccgcca gcgttttgtt gggcgcaagt accatggttg
     3541 ggcgctgaag gtcagcaatg acattggcaa tggtgaaggt tttccctgag ccagtcacgc
     3601 caagtaacgt ctggtgcgcc aggccatctt ccagcccctc ttcgagacgt cgaatcgcct
     3661 ctggctgatc gccagaaggt ttaaaagcgg aattcagttt gaacggttta ctcatgagtc
     3721 gctacctgaa ggagttgggc gggcaggtat gtaattttac tcgtcgtact taattatgcc
     3781 aacaaattat actggataaa aaaacagttc atcaccataa tatttctgat acagcgtaaa
     3841 ctccgctgtc aatcatgagc aaaatttact ctgtggcgag ataaaactcc ggccttaccg
     3901 ggttatcccc aaagcaacgg cttttttaac atttgtcaag atgagtgatg acagttttat
     3961 ggcaagagat gcctgttcag tgactgccat tgcatttatc taaccagtta aaaaataaaa
     4021 gatatttctt tgagccgtct ttaacgccaa ttcgcgtgca agccgcgtat tctctcgctt
     4081 gcctcgtgtt ttctaactct aatacacatg gttatccaca ggaatagtgg ataactgtct
     4141 ccagccccta tcccccgccg cctgggcaat tcccacattc gccgcatgag gcggaagtaa
     4201 aatttttttc gtgattattt tagaatttaa ttggttaaat tgcagtcaat cgaagacgcg
     4261 atctcgctcg caatttaacc aaatacagaa tggctacaac aaggcaaggt ttatgtactt
     4321 tccggttgcc gcattttctg gattttctgc aagccagggg atctctccca gcagcggcgc
     4381 gggaatcatg cgggtgagcg tggtcatata ttcagcgtga cgttttcccg gaggcgtaac
     4441 atcgttcgcc acccaacccg ccagagtcag tccggcgtgt tgtattgcct gcgcagtcaa
     4501 catcgcgtga ttaatacagc cgagtttcac accaactacc agtatcaccg gcagttgttc
     4561 ctgtgttacc caatctgcaa aagtgaaagt gtcagaaagc ggcgtaaacc aaccgccagc
     4621 accttccact aacacccagt cagcctgttg ttcaagcgcg cgtaatccgg cgctcattac
     4681 caatgattct atcggtctgc cctcttgcgc gctgatgatg tgcggcgaag tgggttctgc
     4741 gaaggtgtaa ggatttactg ttgcgtaatc cagctgcagg ctgctgttgc gctgtaacgc
     4801 cagcgcgtcg ctattgcgta aaccttccgg ggtcttttcg ctgccagagg cgaccggttt
     4861 ataacctgcc gtccggtagc ctgctgcctt tgcggcttgt aaaagtgcac aactggcgac
     4921 agttttcccc acttcggtat ccgttccggt gacaaaataa cgtttactca cgagcaatca
     4981 ctcccaaaaa aagatgatac gtcagaggat atcgcccctg ctgttgcggc caggccagtt
     5041 gcaatcgctg caactgcgaa cgcgttaata ttcgcgggtc gcgcccttca tgaagatgcg
     5101 tggcaccgat gcctttcagc gaacgcatgg cactgagcgc atcatcaaac cacagcgtga
     5161 tgggctgaat atgatgttga taatgcacgc cgttcagcga ctgttcgatt tcatctggcg
     5221 gtaaaaagcg attagcatgc ggacgctcgt ccaccgcctg ccacgcctga tgcagttcgg
     5281 gtaacgatcc ctgcaccagc gtggtaaacg cgaccacgcc tttggggcgc accacccgat
     5341 acagctcgcg gagtgccgtg gataaattac cgcaccactg cactgcgaga ttgctccatg
     5401 caagatcgaa cgtcgcagtc gctaacggca gggattcgat atctcccgcc agataatggt
     5461 ctgcggcatc cttctggcgt gcctgaacaa gcattggcgg cgagagatct aaggccgtca
     5521 cctgcgcgtg acgttcccgc cagtggcggc tcatccagcc aggtccacaa cccgcgtcca
     5581 gtacgtgggt gtatttacgc tgtggaagca ttgccagtaa ggcgtcagca ctctggcgct
     5641 gtagatctgc atgttgctca tagtgtgcgg ctgcccgacc aaatgccgct gcaatggctt
     5701 gtttattaac cgttgccatg cagcacctcc agcagacggt cgatatcctg catttcatgc
     5761 gcagcggtta gcgttaagcg cagtcgcgca gtaccagcgg gtacggttgg cgggcgaatc
     5821 gccgtgaccc agcagccttg ctggcgcagt ttttctgcca gttgtaacgc acggctgtta
     5881 tcaccgacaa tcaatggctg gatggcgctg catgaatcag caagcgtaaa cggcaaatcc
     5941 tgtactccgg cacgaaaacg cgtaatgagt gccgccagtt tttcgcgccg tgcatcaccc
     6001 tcatcactgc gaatgaccgc cagcgacgca cgtaatgcct gcgcctgagc gggcggcata
     6061 ctggtgctgt agataaggtg gcgggcgaat tgcagcagat aatccgccac cgtactggag
     6121 caaagcaccg ctgccccgct gacgccaaat cctttgccaa aagtcactac cagcaattct
     6181 ggttttacct tttgcagcca gcagctgccg cgcccctgct ccccgataac gcccgtgccg
     6241 tgggcatcat cgaccatcaa ccagccattg tgctgttgcg ttacctgctg gatttccgcc
     6301 agtggcgcac tatcgccgtc catgctgaac acgccttctg tcaccaccat ttgctgcccc
     6361 ggacaggggg aagcaagcag tcgcgccaga tgagtgacat cgttatgaac aaaacggcga
     6421 agctgcgacg ggcttaaact ggcagcctcc agcaatgagg catggctaag ccggtcggca
     6481 acaatacggt cctctttcgc catcatcgcg gcaatgaccg cctgattagc ggcaaaacca
     6541 gagataaaca gcagtgcccg cgaatagccc agccactcgg ccagttcttc ttccagcgcc
     6601 tgatgtgcca cgctataacc gctgacgtga ccggagccgc cgctaccgac gccaaattgc
     6661 tctgcactct gcttccaggc acggataatt tgcggatgat ggcttaaacc taaataatcg
     6721 ttactggaaa agttcagata ctggcgatcg tccgccacca gccagcgtcc tgctccttgc
     6781 gccactggat aacggcgacg cagggcatcg gcagcacgcc gcgcatcgag ggccgcgttg
     6841 attttatcct gccagctcat agtgctgccg cgttgtaata ttcgtcagtg tccggggtca
     6901 tcagtgcctg ttccagacgc tgctgttgtt cgttatcgcc cgccagcacg gcagtttgct
     6961 gcggatttag ccccagtttg cggaacagtt gcaggtcttt atcttcttcc ggattcggcg
     7021 tggtcagcag tttgcaaccg tagaaaatcg agtttgcgcc tgccataaag cacatcgcct
     7081 gagtctgttc gttcatctgc tcgcgtccgg cagaaaggcg cacgtaagag gttggcatca
     7141 tgatccgcgc gaccgcaatg gtgcgaataa aatcaaaggc atcgacatca tcgttatcgg
     7201 caagcggcgt gcctttcacc ttcaccagca tgttgattgg cacgctttcc ggcggcgtcg
     7261 gcaggtttgc cagttgcagc aataatccgg cgcgatcttt taccgtttcg cctaagccca
     7321 caatgccgcc agaacagact ttgatcccgg catcgcgcac tttttccagc gtatcgaggc
     7381 gttcctgata agtgcgtgtg gtgatgatat tgccgtaaaa ctccggcgag gtgtccaggt
     7441 tgtggttgta gtaatccagc ccggcgttcg cgaggcgctg cgcctgagat tcactcaacg
     7501 tgcccagcgt catacacgcc tccagcccca tcgcttttac cccctgcacc atttgttcca
     7561 ggtacggcat atcgcgttcg tggggattct tccacgccgc gcccatacag aagcgcgtcg
     7621 atcctgccgc tttcgctttg cgcgccgact ccagcacctg ttcaacttcc atcaaccgct
     7681 cggcttccag cccggttttg tagcgcgagc tttgcgggca gtatttgcaa tcttccggac
     7741 aagctccggt cttaatcgac agcaaagtgc tgacctgcac ctgacgagga tcgaaatgct
     7801 gtcgatgtac ctgctgcgct tcaaacagca gatccagcaa cggtttttca aataattctg
     7861 tgacttgcga caatgtccag cgtgggcggt gagccatggg gcttctccaa aacgtgtttt
     7921 ttgttgttaa ttcggtgtag acttgtaaac ctaaatcttt tcaatttggt ttacaagtcg
     7981 attatgacaa cggacgatct tgcctttgac caacgccata tctggcaccc atacacatcc
     8041 atgacctccc ctctgccggt ttatccggtc gcgagcgccg aaggttgcga gctaattttg
     8101 tctgacggca gacgcctggt tgacggtatg tcgtcctggt gggcggcgat ccacggctat
     8161 aatcacccgc agcttaatgc ggcgatgaag tcgcaaattg atgctatgtc gcatgtgatg
     8221 tttggcggta tcacccatgc gcccgccatc gagctatgcc gcaaactggt ggcaatgacg
     8281 ccgcaaccgc tggaatgcgt ttttctcgcg gactccggct ccgtagcggt ggaagtggcg
     8341 atgaaaatgg cgttgcagta ctggcaggcc aaaggcgaag cgcgccagcg ttttctgacc
     8401 ttccgcaatg gttatcatgg cgataccttt ggcgcgatgt cggtgtgcga tccggacaac
     8461 tcaatgcaca gcctgtggaa aggctatctg ccagaaaact tgtttgctcc cgccccacaa
     8521 agccgtatgg atggcgaatg ggatgagcgc gatatggtgg gctttgcccg cctgatggcg
     8581 gcgcatcgtc atgaaatcgc ggcggtgatc attgagccga ttgtccaggg cgcaggcggg
     8641 atgcgcatgt accatccgga atggttaaaa cgaatccgca aaatatgcga tcgcgaaggt
     8701 atcttgctga ttgccgacga gatcgccact ggatttggtc gtaccgggaa actgtttgcc
     8761 tgtgaacatg cagaaatcgc gccggacatt ttgtgcctcg gtaaagcctt aaccggcggc
     8821 acaatgaccc tttccgccac actcaccacg cgcgaggttg cagaaaccat cagtaacggt
     8881 gaagccggtt gctttatgca tgggccaact tttatgggca atccgctggc ctgcgcggca
     8941 gcaaacgcca gcctggcgat tctcgaatct ggcgactggc agcaacaggt ggcggatatt
     9001 gaagtacagc tgcgcgagca acttgccccc gcccgtgatg ccgaaatggt tgccgatgtg
     9061 cgcgtactgg gggccattgg cgtggtcgaa accactcatc cggtgaatat ggcggcgctg
     9121 caaaaattct ttgtcgaaca gggtgtctgg atccgacctt ttggcaaact gatttacctg
     9181 atgccgccct atattattct cccgcaacag ttgcagcgtc tgaccgcagc ggttaaccgc
     9241 gcggtacagg atgaaacatt tttttgccaa taacgagaag tccgcgtgag ggtttctggc
     9301 tacactttct gcaaacaaga aaggagggtt catgaaactc atcagtaacg atctgcgcga
     9361 tggcgataaa ttgccgcatc gtcatgtctt taacggcatg ggttacgatg gcgataatat
     9421 ttcaccgcat ctggcgtggg atgatgttcc tgcgggaacg aaaagttttg ttgtcacctg
     9481 ctacgacccg gatgcgccaa ccggctccgg ctggtggcac tgggtagttg ttaacttacc
     9541 cgctgatacc cgcgtattac cgcaagggtt tggctctggt ctggtagcaa tgccagacgg
     9601 cgttttgcag acgcgtaccg actttggtaa aaccgggtac gatggcgcag caccgccgaa
     9661 aggcgaaact catcgctaca tttttaccgt tcacgcgctg gatatagaac gtattgatgt
     9721 cgatgaaggt gccagcggcg cgatggtcgg gtttaacgtt catttccact ctctggcaag
     9781 cgcctcgatt actgcgatgt ttagttaatc actctgccag atggcgcaat gccatctggt
     9841 atcacttaaa ggtattaaaa acaacttttt gtctttttac cttcccgttt cgctcaagtt
     9901 agtataaaaa agcaggcttc aacggattca tttttctatt tcatagcccg gagcaacctg
     9961 tgaacacatt ttcagtttcc cgtctggcgc tggcattggc ttttggcgtg acgctgaccg
    10021 cctgtagctc aaccccgccc gatcaacgtc cttctgatca aaccgcgcct ggtacctctt
    10081 ctcgcccgat tctgtcggca aaagaagcgc agaatttcga tgctcaacac tattttgcat
    10141 ccctgacacc aggtgctgca gcgtggaatc cttccccgat taccctgcct gcgcaacctg
    10201 actttgttgt cggcccggcg ggcactcaag gtgtaacgca taccacgatt caggcggcgg
    10261 tagatgcggc aattatcaag cgtaccaaca agcgccagta tattgccgtg atgcctggtg
    10321 agtatcaggg aacggtatat gtccctgccg ctccgggtgg aattactctg tacggtacag
    10381 gtgaaaaacc gattgatgtg aagattgggc tttcccttga tggtggcatg agccctgccg
    10441 actggcgtca cgacgtcaac ccgcgcggca aatatatgcc aggtaaacca gcgtggtata
    10501 tgtacgatag ctgccagagc aaacgcagcg acagtatcgg tgttctctgc tctgcggtct
    10561 tctggtcaca aaacaatggc ctgcaactgc aaaatctgac catcgaaaac acgctgggcg
    10621 atagcgtaga tgcaggtaac catccggcgg tggcactgcg tactgatggt gaccaggtac
    10681 agattaacaa cgttaacatt ctcggtcgtc agaacacctt ctttgtcacc aacagcggtg
    10741 tgcagaaccg tctggaaacg aatcgtcagc cgcgtacgct ggtgaccaac agctacattg
    10801 aaggggatgt ggatatcgtt tctggtcgcg gcgcagtggt gttcgataac accgaattcc
    10861 gcgtggtgaa ctcacgtact cagcaagaag cgtatgtgtt tgcaccggct acgctgtcca
    10921 acatttacta cggtttcctc gccgtaaaca gccgtttcaa tgctttcggt gatggtgtgg
    10981 cgcaactggg ccgctcgctg gatgttgatg ccaataccaa cggtcaggtg gtgatccgtg
    11041 atagcgccat caacgaaggt tttaacacgg ctaaaccgtg ggccgatgcg gtgatctcta
    11101 atcgtccgtt tgcgggtaat accggcagcg tagatgataa cgacgaaata cagcgcaatc
    11161 tgaatgacac taactacaac cgcatgtggg aatacaataa ccgcggcgtg ggtagtaaag
    11221 tggttgcaga ggcgaagaag taagagcaat taactatttg ccggatgcgg cgtaaacgcc
    11281 ttatccggcc tacggttcga tgcgatttgt ag
//
LOCUS       DACAPB010000147        11305 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.147, whole genome shotgun sequence.
ACCESSION   DACAPB010000147 DACAPB010000000
VERSION     DACAPB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.147"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(162..675)
                     /locus_tag="IT904_003490"
                     /pseudo
     CDS             complement(162..675)
                     /locus_tag="IT904_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(842..1165)
                     /locus_tag="IT904_003491"
                     /pseudo
     CDS             complement(842..1165)
                     /locus_tag="IT904_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1911..2888
                     /locus_tag="IT904_003492"
     CDS             1911..2888
                     /locus_tag="IT904_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP3193229.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3173..3913)
                     /locus_tag="IT904_003493"
     CDS             complement(3173..3913)
                     /locus_tag="IT904_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP3193230.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4034..4222)
                     /locus_tag="IT904_003494"
     CDS             complement(4034..4222)
                     /locus_tag="IT904_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HAP3193231.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
     gene            complement(4596..5504)
                     /locus_tag="IT904_003495"
     CDS             complement(4596..5504)
                     /locus_tag="IT904_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HAP3193232.1"
                     /translation="MTLSIKNIKRIITAWKPSTFETYKKTFEKYGGSVNMHPDVVSYF
                     MIHHDWKFDFFHYEKDGDIKGSYFLCNGKQIGIMARRSYPLSSDEVLIPFSPHARCFF
                     PDKTNKLSIINKQNIINATWKIARKKQNCIIKESFSPKFEKTRRNEIQRFIRNGGEIK
                     CISQLSDKEISSSYISLFHSRFGGTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCA
                     IDIVLKSESSCNVYYDVPNGAVLNDENCMKLSPGSVLMWLNIDKARRYCQDNNKKMIY
                     SIGAFRPEWKYKLLWSVPCKVGKCLC"
     gene            complement(5567..6676)
                     /locus_tag="IT904_003496"
     CDS             complement(5567..6676)
                     /locus_tag="IT904_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004137295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP3193233.1"
                     /translation="MKLLLLTGATGFLGGAVLDKLLDNCNNINLLLLVRAPTPQAGLE
                     RIKENMRKFNVCEERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGN
                     NPFIWNVNVTGTLAFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHL
                     VEYTHSKATIEYLMRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKF
                     MCSLDDKIDVIPVDYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALN
                     CDPVGDRYTKVSYDILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLL
                     SIGMPKPPKFTDYIKYCIETTKHLSIQQQMEVDFK"
     gene            complement(7109..8062)
                     /locus_tag="IT904_003497"
     CDS             complement(7109..8062)
                     /locus_tag="IT904_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HAP3193234.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISDHTKNGAGIESYNFMTTAGLKYYF"
     gene            complement(9335..9493)
                     /locus_tag="IT904_003498"
     CDS             complement(9335..9493)
                     /locus_tag="IT904_003498"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3193235.1"
                     /translation="MAQNAFIESFNRTYRTKILGFCLFRTLDEKRELAANWLSEYNSE
                     RHINYLTI"
     gene            9677..10890
                     /locus_tag="IT904_003499"
     CDS             join(9677..10001,10001..10890)
                     /locus_tag="IT904_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP3193236.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEF
                     DRLWKKLMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVVAGDRVNRQFVAERPDQLWVADSTYVSTWQGVVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     gene            complement(11038..11305)
                     /locus_tag="IT904_003500"
                     /pseudo
     CDS             complement(11038..11305)
                     /locus_tag="IT904_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3128 a         2540 c         2412 g         3225 t
ORIGIN      
        1 tttttcggtt gaaaagaaat tgattgaatt actggaaaaa caatctggtc tccatacgtc
       61 tgtggattgc agtactcccc ttacagagca gagtctgtcg agaaatgacg gtgaagacca
      121 gaccagaaga aacgctgaaa atatgttcgg agatgactga actaacccag cctggccagg
      181 ggaatatctc gccatttagt tgtataacac tgactcaaca tttcccgttt catttgccat
      241 tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttcccgactg gttcagttca
      301 tcgagtgttt tcattaactt ttcactgttt ttacggggct ggatttcatc aaataatccc
      361 ggctgcgcta tacccgatgg tgtgaaatca gccagcatga ctcctgcctt catatagcgg
      421 tacccttcac gccagacatg gtttaaggct ctgcatgcgg cggcaataat gtcccggctg
      481 tcctgtgtgg gcaatgaaag cttttccaca gcggcattgc tgtaacaggg ttcaggggga
      541 tgtccgtaca aatgtcgtca cctgccggca atactgacgc tccccacgta gtttctctgc
      601 ggcccgctct gcatactgaa caacagcctg gtgcatggca tctttgtctg tgattcgttc
      661 accaaaactg cgactatgtc aacgccacga tgtttgaccg ttatttgcca ttttcatgcc
      721 cgctaaatac tttcgttact gatgctgtcc gctttgcacc atttcatcat cactgattgg
      781 cgttgtgttt ttggtcagca ctccagcttt acgtttctct tttaaccggt aactctctcc
      841 ttgttctttt tgacggacgc attttacgtt tgggctggat gtagtaacgc agcatggaac
      901 gtccgcccgt ataacccatt gccttaatct ccgcgaagat aacctcgcta ttccagacat
      961 tctctgccag gcgcatgtcg atgtaatcca taaacggttt cagcttaacc attttgtggc
     1021 gggtctttct ggctggcggt tcagggtatt tgaggtagcg tctgaccgtc cgttcagagc
     1081 aaccaatctg agccgcaata tcgacaatgt acgcgccctg ctggcgcatt tgctttatca
     1141 tgtaaaagtc ctctctgctc agcatgttga tgtcctttct ggtgtgagaa ccttaaggaa
     1201 acaacatgtt gggtggagcg gacaatccaa atggtgaatt accgtcttat atcactggcg
     1261 ctgacacctg tagtttgtat acacaatccc tggcattgaa acacacatat gaagtgtact
     1321 tcggatatga ccgtacgctc aggaagtacg gcagattccg tcaaaaatta cctgtaaaga
     1381 cagacatcag aaaaaacagt gctcgccgca gaccggtgac cgggcgaagc ccgcgaaccg
     1441 gcgaggaagc gcaatgtatc tgtagttaca gggagcaggt acgccctgaa gccctttcat
     1501 aaaacggttg tgtgcgctgt ccttctggaa tgatttatcc acatatccgg aggcccgatc
     1561 cagtaaacag atccatgaat gatcaacaaa ggatccatta aagatcccca tatcgctgca
     1621 aaccttgtca ctcatgggcc gggaccacga tcacataagc tgtggcatgt tactgataaa
     1681 ctgtaacatg ctaatgataa gctgtattca gtaatccata tactgaagta agttaatgac
     1741 ataaactatg gtcagtatgc cagactcagt tgttaaatac aggctgcagg tttttcttca
     1801 gtcagttagc ggagttctga cacacgattt tctgtttatt cttttactgt ccacaggctg
     1861 gaggctttct ggaaaacgaa aattcagaca tcaaaaaact gttcggcgag gtggataagt
     1921 cctccggtga gctggtgaca ctgacaccaa acaataacaa caccgtacaa cctgtggcgc
     1981 tgatgcgtct gggcgttttt gtaccgaccc ttaaatcact gaagaacagt aaaaaaaata
     2041 cactgtcacg tactgatgcc acggaagagc tgacacgtct ttccctggcc cgtgctgagg
     2101 gattcgataa ggttgagatc accggccccc gcctggatat ggataatgat ttcaagacct
     2161 gggtggggat cattcattcc tttgcccgcc ataacgtgat tggtgacaaa gttgaactgc
     2221 cttttgttga gtttgcaaaa ctgtgtggta taccttcaag ccagtcatcc cgcaggctgc
     2281 gtgagcgcat cagcccttcc ctgaagcgca ttgccggtac cgtgatttcg ttttcccgta
     2341 ccgatgagaa gcacacccgg gaatatatca cccatctggt acagtcagct tactacgata
     2401 ctgagcggga tattgtgcag ttacaggccg atccccgcct gtttgaactg taccagtttg
     2461 acagaaaagt ccttctccag cttaaggcga ttaatgccct gaagcgacgg gagtccgccc
     2521 aggcactcta cacctttata gagagcctgc cccgggatcc ggcaccgata tcgctggcgc
     2581 ggctgcgtgc acgcctcaat ctgaagtctc ctgtattttc ccagaaccag acggtcagac
     2641 gggcaatgga gcagttgcgc gagattggat atcttgatta cacggagatc cagcgggggc
     2701 ggacaaaatt cttctgtatt cactaccggc gtccccggtt aaaagcgccg aatgacgaga
     2761 gtaaggaaaa tccgttgcaa ccttcacctg cggaaaaagt cagtccggag atggcggaga
     2821 agcttgccct gcttgagaaa ctgggcatca cgctggatga cctggaaaaa ctcttcaaat
     2881 cccgctgaac ataaactgta gtcagtgaag agcgctcctt tactgactac agcttatatt
     2941 aaccggtgca gtgagtggtc tgctcactgc agtttatatt ctgtttcctg cagtgctgtc
     3001 tgtagctgag ctgccatctg cctgtccctt acgtaagtga tcccgtaacc tgatgctgag
     3061 gcattgctcc cttcataaaa catgacttac tcactacagc ttatatacat gctccagctt
     3121 atgttatgcc tgttctgctg accacagctt atataaggaa gcgcgtatgc cgtcacctca
     3181 ggggaggcag tgtacgcagg atctccgcag catcccgccc gtcacctgtg aaaggcactg
     3241 ccagcgtggc agccatatcc agtgcaaaca ctctggtata aacctccatc gaacgtggat
     3301 ccctgtgacc aaccagtgcc tggatgactt tccggggctg gcggtgatag agcatgtgca
     3361 tgatatagct gtgccggaag gtgtgtggtg tgaccggaat cgaaaagtgt actccgtcag
     3421 cttcggcccg tctgacagcc tgcttcagcc agttgcgcat ggtttcgtcg gtcacggccc
     3481 ataatggttc acgacgacgg ggccgggtgg tgatcatcca gctttccatc tgcctgacat
     3541 agcttatatc tgtcagcgga accaggcgca cttcatcttt tggcgggcgt ccgcgtcgcg
     3601 cacgcacttt ttcggacagg atccgcacaa acggtcttac tccattcagg tcaaatgatt
     3661 ccggcgtcag catccgggct tcgccaatac gcattccggt attccagagg gtggcgaaca
     3721 gcatatggtg acgctgatcc ggcatataga agagaagggc actcacttcc ggggccagca
     3781 ggtaggccgg tgtggccccc gtggaagtgg ccattcttct cagggagagc gctgttgcaa
     3841 aatccacccc cggcgcaatg ggtaacaggg agacgcgttc aggtgaattc tgcaggggaa
     3901 tgacattgtt catgagttat ttttcacgta gcacacgcac tgtttatgga tacaggtatt
     3961 ctgacctgaa cgcccggatc cttccacgac aataaatcgc agcgcgcgga tccttttgtt
     4021 tgcttatcac gcattattgc gggtaaaacc aaatacgtaa aggcgcgttt tgccgcaggg
     4081 atcctgtttt aagccagtca tggtgcgggc tgaggactgg tttgccgttt ttgggagaat
     4141 tggggtaatg ccaggacagg cagaaacgga gaaaaaatcc tgcagacgcc cttaaaagac
     4201 aactgaaaat ttctgactcc atattcctct ggtttcagac tgcttaatca catcatcaac
     4261 ctgatacata acaaacagat tctgttcaca gaatgaacaa ttaagtaaat ggttagatta
     4321 tgcgaaaaag tacagatatt tctcaccatc gttattgaat aatatgtctt cacacaaaca
     4381 atattaatat tttgagtgta agccctgact caaagaaaga aatgtctgtt ttcggaatca
     4441 aaaatatata gttatcaaaa tacattaatt tcaaatttga aattaatgta aaacgattta
     4501 gtaaatgtta tgatattata aatctactcc tccatgttta gaggagtatt tgcagactgg
     4561 acgctcttat atagttactt acctttatac atattctaac ataagcattt accaacctta
     4621 cagggaacag accatagtag tttatatttc cattcaggtc tgaatgcacc gatagaatat
     4681 atcatctttt tattattatc ctgacaatac cttcgcgctt tatctatatt cagccacata
     4741 aggacacttc ccggacttag tttcatgcaa ttctcatcat tcagaactgc accatttgga
     4801 acgtcataat aaacattaca tgagctttca gatttcagta ctatatcaat agcacacggt
     4861 ttattatccc agaatagcac atggccaaac ataagttctc gtaagtgaga aataaacatt
     4921 aacagattat catattcgta gcaaggtagg gtacctccaa accgggaatg aaaaagtgat
     4981 atataagagc tggatatttc tttatcggat aactggctta tacacttaat ctctccgcca
     5041 ttcctgataa atctctggat ttcatttcgg cgagtttttt caaatttagg tgaaaaagat
     5101 tcctttataa tacaattttg tttttttctg gctattttcc atgttgcatt gataatattt
     5161 tgtttattta taattgataa tttattagtt ttatccggaa aaaaacatct tgcatgtggg
     5221 gaaaaaggaa ttaatacctc atcagatgaa agaggataag accttcttgc cattatccca
     5281 atttgttttc cattgcacag aaaatatgag cctttgatat caccatcttt ttcatagtga
     5341 aaaaaatcaa acttccagtc atgatgtatc ataaagtatg aaacaacatc cggatgcatg
     5401 tttacactac caccgtattt ttcaaaagtt tttttgtacg tttcgaatgt agatggtttc
     5461 caggccgtaa taatgcgctt tatattttta attgacaaag tcatatcctc ggcgttttta
     5521 tatattatga gtgcaacacc aacaataatt ctgattatga taaatattat ttaaaatcaa
     5581 cttccatttg ttgttgaatt gaaaggtgtt ttgtcgtttc tatacagtat ttaatataat
     5641 cagtaaactt tggcggttta ggcattccga tactcagtag cttgtcgtta ctgaaacaaa
     5701 cattgagcat actgaacgct ccatataaac gaatggcttt taacataagg cgttcgttac
     5761 agggaccaaa aatattttta aaatcatgac ggctcattgc cagtatgtca taactgactt
     5821 tagtatatct gtctccaaca ggatcacagt tcaaagcacg ggctacagct tcgtcaatag
     5881 cagagaacgt cacactactt tctttacctg ctgatatatg aacaatctca ccattaatta
     5941 acgagctttc aagcaacatt agcaatgcat cagcacaata atctacaggg ataacatcta
     6001 ttttatcatc cagagagcac ataaattttt gcaacattaa ccccattctg aatacccaga
     6061 aaatactggt tgaaggtaag caccctaaac gactgtggcc aacaataatt gatggtcggg
     6121 caaccaacaa aggtaaatca ggacactgct tacgcatcag atattctatt gttgcttttg
     6181 aatgcgtata ctccactaaa tgttcacctg tttctgatga agcagactct tccttaacta
     6241 gcgaccccgt atgaggtgta caagacatag cagtaccaac atgaaggaag cgtttcagtc
     6301 ctgccacttt tgccattctt cttgcaaaag caagtgtacc tgtaacattc acattccata
     6361 taaaaggatt attaccaaaa gaagctatag ccgcacagtt tataacatga gtgacttcat
     6421 caagacgagg atccatgaga aaggcttccg gattatttag atccccaggc aagatgttat
     6481 cattagttaa tgcatgcaac ctttcctcac aaacattaaa tttacgcata ttttctttaa
     6541 ttctttccag tcccgcttgt ggagtaggtg ctcgtactaa aagtagcaaa tttatattat
     6601 tacagttatc cagcagctta tccaggaccg cgccaccaag aaatcctgtt gcacctgtaa
     6661 gtaataataa tttcatatct aacctctttt gttttcatgt gatcattata aaaataaatt
     6721 atcttacata aacaatgatt aaacaaaatc aagagagatt aaatgtaaaa acaatgttta
     6781 tttttatacg gcagaatcct tcaggttatt gcgtgtgttt ttattggatg gtcttcttat
     6841 tattgacgag taatagaatt ataataaagt tcatattgtt aacatcgctt atcaattatt
     6901 tttatgtcga taacactgtg tcgttatgca tgaagagtgg cagggcagca tctttaaaaa
     6961 tacaagagtt aacagaaaca cttttatcgt tgagcacata taccctccac atattataaa
     7021 aaatcaaaag accaccatca gaagtgctgg ccttttgttt atcatattta aagtgtcatg
     7081 aaaaagttaa aaacaggcat gcttcaggtt aaaaataata cttcagacca gctgtggtca
     7141 taaagttata gctttcaatg cctgcaccat ttttggtgtg atcagatata ttcaaatttc
     7201 tggagtacag agaagtatca cctttcttat tagtaatacg gttccatgtt ccttcgacat
     7261 aaactttagc tgcaggtgta atataataac cagcatgtag tgagacagag taatagttct
     7321 gattatttac atcgctacga taggtgatcc ttttctcagg gttgtaatgc tcatcattat
     7381 ccgatgcctt cacccatccg ctatatttga aactcccccc gaactcaaag ctatcatacc
     7441 gataattacc ggttaaccct atatagggca ttttaaagtg ttgtttgtag cctatagctc
     7501 tttcaccatc tggaaatgat cccgtttcat cccggaagcc tccctcacta ctatagatat
     7561 aagagcctcc ttttgctgta aagctataac gattttcctg gtaaccagcc ataagaccta
     7621 actgatagtc tggttgattc aggagccatc ccttaatatt caggtcgaat tcattagcaa
     7681 aatttagacg ggtattcgga tgtttactct catcagtcca ggttccggga ttgctcgtat
     7741 ccagccagtc cctgtccacc atattgcctc cacgaccagc taatgttgtc caccctgagg
     7801 caccgacaga tactctgggt aacagatccc agttaaatgc ccccttgaca attggcgcat
     7861 tactgtattt ccagtccagc tggctggctt tgcgcccttt ttcttcaggc agataaaccc
     7921 gttctttggc cttgccactt aaagtgccaa aatcaatctc cgtactgatt ttctccggtg
     7981 taaaagaaag cccagtatca gaagccagtg cagcaaacgc tacaggagct gagagtgcag
     8041 tagcaagaat ctttaagtac atagacaagt taactccatt ccactcgttt gtttttgata
     8101 aatatttttt tatttactcg tagtagtcaa ctgaaaaatg ctgttttgtt taacgtttgt
     8161 ttacaatgtg gccacatgtc gctttattta ttccctttta tgtcagaata tcaggggagt
     8221 cagtcaaacc cggtatgatt actgccccgg tcatccatgt tccagaaaat atcagggcat
     8281 acagggcgct ggtaactgcg aataaggccc tggtcggaca ctttatgccg ggaacgtctg
     8341 taacgtggaa accggtacct tcttcctggg gttattttgt taactcttat ctaaagggag
     8401 acaaaatggc caaatcctgc gattatgaag caatcagggt ctttattagc gttaatgtcg
     8461 gcaaatatac caataacgcc attacagtta attattcaga tagcgtttgt gcgacaagac
     8521 attaccattc gacgaaaata aactcaggtg gttgatatca gaaatcaacc gtggctccac
     8581 ctacaaaaaa agttgctggt ttataaatcc ctcctttctg attaataaga aaactaataa
     8641 catatgagtg tgattacttg ttcagataag ttaattatct tagtgattga tatttatttt
     8701 gtttcagtga aaatgatgct tttattagaa aatttataaa taccaagtcg gtggtgttta
     8761 ttagtgtaat ggaggggagt gcgtaaatcg ttcatgtaac acttccttcc aataatactt
     8821 tataaatcaa tagatgtatg attacttatt tcctgaaata tcatatatat tgctatattt
     8881 cttaacgaga tattcttatt ttattgataa ttgcatctct tttatttaat gtaaagcttt
     8941 cttattaatc ttctaatgta ttgcaaaatg gtagatgctg agtaatcaac agacttgtat
     9001 gtaaagtcaa gattaacgtt gctaaagcac gcatcaattt tctgataaaa ggatggggtt
     9061 gtttttttcg ataaaaaaaa ttcttgtcta accactggtg aaataagatt attattaccc
     9121 cgaattaata ctgagcctaa caatagaata caaaaaaaac aaaatgcttt aaataagggg
     9181 agcactataa gttatcctct ttactttatg cttaccaact cacttatgtt actttataac
     9241 ataacccatc ttgcgttgta aacagactcg ttttagttcc atgcaccttt tgagatcgtg
     9301 accaaataat ggcgttagtg atgttcttcc agtgtcatat ggttaagtag ttgatatggc
     9361 gttcactgtt atattctgac aaccagtttg ccgccagttc ccgcttttca tccagcgttc
     9421 tgaacagaca aaaaccgagt atttttgttc ggtatgtccg gttaaaactc tcgataaaag
     9481 cgttctgcgc caacttacct gattgggtaa tctccagttt tactacatgt ttttctgcgc
     9541 attacgtccg tgttggataa aaaaaatcct ggccataatc tatgcgaatc atagctggat
     9601 agacatggtt ttccgcaatc cttgaaccgc cccgggaatc ctggagacta aactccctga
     9661 gaaagaggta aacaggatga ctaaaaatac tcgtttttcc cccgaagtcc gtcagagggc
     9721 gattcgtatg gttctggaaa gtcagggcga atatgactca cagtgggcgg caatttgttc
     9781 cattgcccca aagattggct gtacaccgga gactctgcgt gtctgggtac gccagcatga
     9841 gcgggatacc ggaggcggtg atggcgggct caccaccgct gaacgtcagc gtctgaaaga
     9901 gctggaacgt gaaaatcgtg aactgcgccg cagtaacgat atccttcgcc aggcttccgc
     9961 ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa tgatgccact gctggataag
    10021 ctgcgtgagc agtacggggt cggaccgcta tgcagcgaac tgcatattgc cccgtcaacg
    10081 tattaccact gtcagcaaca gcgacatcat ccggataaac gcagtgcccg tgcgcagcgc
    10141 gatgactggc tgaagaaaga gatacagcgc gtatacgatg aaaatcacaa ggtatacggt
    10201 gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca gagtggccag atgcactgtg
    10261 gcacgtctca tggcggttat gggacttgcc ggtgttctcc gtggtaaaaa ggtccgtacg
    10321 accatcagcc ggaaagccgt tgtcgcaggc gaccgcgtaa accgtcagtt cgtggcagaa
    10381 cgtcctgacc agttgtgggt ggctgattct acttacgtca gcacatggca gggggtcgtc
    10441 tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg tggggtggcg ggtctcatcg
    10501 tctatggaaa cgacatttgt gctggatgct ctggagcagg cgttatgggc ccgtcgaccg
    10561 tccggcacag tccatcacag tgataaaggt tctcagtatg tatcgctggc ctacacacag
    10621 cggcttaagg aagccggatt actggcatca acaggaagta caggcgactc gtatgacaac
    10681 gcgatggcgg agagcatcaa tggcctttac aaagcggagg taatacaccg taagagctgg
    10741 aaaaaccgtg cagaagtgga actggccaca ctcacgtggg tggactggta taacaatcga
    10801 cgattgctgg aaaggctggg ccatactcct ccggcagaag cagaaaaagc ttattatgct
    10861 tccatcggaa acgatgatct ggcagcctga gttcacagat aaaacactct ccaggaaacc
    10921 cggggcggtt cacctacaga gtaccgctac cacttgtgaa gctggcagat tcagattgtt
    10981 ttcaatcgac aacgcctcat agttaaagtc atcaacgaca ttgaacgtgt gaagtggtca
    11041 acaaaaactg gccaccgagt tagagttttt ccagtatcga ttttccgatt cgtttgggga
    11101 taacccaccg ttatattcgt gcggtcttag tgcgctgtaa tatccaacga tatagaccgt
    11161 tatggcgtga gcggcctcgc agaagcttcg taacccacca ccggcatcca ttcgttcttc
    11221 agactcctga agaagcgttc cattgggctg ttatcccagc agtttccgcg ccggctcata
    11281 ctctgcctga tctggtatcg ccaca
//
LOCUS       DACAPB010000148        11233 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.148, whole genome shotgun sequence.
ACCESSION   DACAPB010000148 DACAPB010000000
VERSION     DACAPB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.148"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..744
                     /locus_tag="IT904_003501"
     CDS             <1..744
                     /locus_tag="IT904_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAP3193237.1"
                     /translation="NYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQLNAALVND
                     AAAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCASDNSHQCPTETV
                     PYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAAVLNAEVG
                     CSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVIVAINKDK
                     NAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            800..2089
                     /locus_tag="IT904_003502"
     CDS             800..2089
                     /locus_tag="IT904_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP3193238.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEHAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILETNVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            2086..2379
                     /locus_tag="IT904_003503"
     CDS             2086..2379
                     /locus_tag="IT904_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP3193239.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWKYPAGTFGIDFRYG"
     gene            2436..4082
                     /gene="fadK"
                     /locus_tag="IT904_003504"
     CDS             2436..4082
                     /gene="fadK"
                     /locus_tag="IT904_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAP3193240.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHTASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4139..6517)
                     /gene="ppsA"
                     /locus_tag="IT904_003505"
     CDS             complement(4139..6517)
                     /gene="ppsA"
                     /locus_tag="IT904_003505"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAP3193241.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            6850..7683
                     /gene="ppsR"
                     /locus_tag="IT904_003506"
     CDS             6850..7683
                     /gene="ppsR"
                     /locus_tag="IT904_003506"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAP3193242.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            7840..8886
                     /gene="aroH"
                     /locus_tag="IT904_003507"
     CDS             7840..8886
                     /gene="aroH"
                     /locus_tag="IT904_003507"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAP3193243.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            9018..9209
                     /gene="hemP"
                     /locus_tag="IT904_003508"
     CDS             9018..9209
                     /gene="hemP"
                     /locus_tag="IT904_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAP3193244.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(9213..10649)
                     /locus_tag="IT904_003509"
     CDS             complement(9213..10649)
                     /locus_tag="IT904_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAP3193245.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(10712..11233)
                     /gene="ydiV"
                     /locus_tag="IT904_003510"
                     /pseudo
     CDS             complement(10712..11233)
                     /gene="ydiV"
                     /locus_tag="IT904_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
BASE COUNT         2772 a         2937 c         2831 g         2693 t
ORIGIN      
        1 aattacgccg aaagcattgc tgctctgctg aaagataaac accccgctat gttgctactg
       61 gccgcgacga aacgtggtaa agctctggca gcacggttaa gtgtgcaact gaatgcggcg
      121 ctggtgaacg atgccgcggc ggtggatatt gtcgatggtc acatttgcgc cgaacaccgg
      181 atgtatggcg ggttagcgtt cgctcaggaa aagatcaaca gctcgctggc gatcattacc
      241 cttgcacccg gtgttcagga accgtgcgcc agtgataact ctcatcagtg cccgacagaa
      301 acggtacctt atgttgctcc gcgtcatgaa attctctgtc gcgagcgccg tgcgaaagcg
      361 gcaagcagcg tggacctgag caaagcaaaa cgtgtggttg gcgtcggtcg tggtctggca
      421 gcgcaggatg acctgaaaat ggtccacgaa ctggcggcgg tgctgaatgc tgaagtcggc
      481 tgttcacgtc caattgccga aggcgagaac tggatggagc gtgaacgtta tatcggtgtc
      541 tccggcgtgt tgctgaaatc cgatctctac ctgacgctgg ggatctccgg gcagatccaa
      601 catatggttg gcggcaacgg cgcaaaagtg attgtcgcca tcaataaaga taaaaatgcg
      661 ccaatcttca actatgccga ctacggtctg gtgggcgata tctacaaagt cgtccctgcc
      721 ctgattagcc agttgagccg ctaattcccc tttcaaccac atctcccgct gtaaacgcag
      781 cgggaaggga gcataacgca tgtcggatga caaatttgat gccattgtgg tcggtgcggg
      841 cgttgctggt agcgttgccg cactggtcat ggcacgagcc gggctggatg tcctggtgat
      901 agaacgcggc gacagtgccg gatgtaaaaa catgaccggc gggcgccttt atgcccacac
      961 acttgaagca atcattccag gctttgcagc atcagcgccg gtagaacgca aggtcacacg
     1021 cgagaaaatc tccttcttaa ccgaagagag cgccgttacc ctcgattttc accgcgagca
     1081 accagatgtt ccgcaacacg catcttatac cgtattgcgt aatcgtctgg acccgtggtt
     1141 gatggaacat gccgagcagg cgggcgcaca gtttatcccg ggagttcgcg tcgatgcgct
     1201 ggttcgtgaa ggaaacaagg tcactggcgt ccaggctggg gatgatattc tcgaaacgaa
     1261 tgtggtgatt ctggctgatg gcgttaactc gatgcttggc cgctcgctgg gaatggttcc
     1321 cgcttccgat ccgcatcatt acgctgttgg tgttaaagag gttattggcc tcacaccaga
     1381 acagatcaac gatcgcttta atattacggg cgaggaaggt gccgcctggc tgtttgccgg
     1441 ttccccttct gacggcctga tgggtggcgg attcctctat accaataagg attccatatc
     1501 gttggggctg gtttgtggat tgggtgatat cgcccatgcg caaaaaagcg tgccgcaaat
     1561 gctggaagat tttaaacaac accccgccat tcgcccgctg attagcggcg gcaaactgct
     1621 tgaatattcc gcgcatatgg tgccggaagg cggtctggca atggtgccgc aactggttaa
     1681 cgagggcgtg atgatcgttg gtgacgccgc aggcttctgc ctgaatttgg gttttacggt
     1741 ccgcggcatg gatttagcca ttgcatcggc tcaggctgcc gccacaacgg tgatcgccgc
     1801 caaagaacgc gcggatttct ccgccagcag tcttgcgcaa tacaaacgtg agctggaaca
     1861 aagctgcgtc atgcgtgata tgcagcattt tcgcaagatc ccggcgctga tggaaaaccc
     1921 gcgcctgttt agccaatacc cacgaatggt agccgacatc atgaacgaga tgttcaccat
     1981 tgacggcaaa ccaaaccagc cggtacgcaa aatgatcatg ggacatgcga agaaaatcgg
     2041 gctgatcaac ttgctgaaag atggcattaa gggagcaacc gcgctatgag ccagaacgct
     2101 acggttaacg tcgacatcaa attaggcgtc aataaattcc atgttgatga gggccacccg
     2161 catatcattt tggcggcaaa tcccgatatc aatgaattcc gtaaattaat gaaagcctgc
     2221 cctgccggac tttataagca ggatgacgcc ggcaacattc attttgattc tgccggttgt
     2281 ctggagtgcg gcacctgtcg ggtgttgtgc ggtaacacta ttctcgaaca gtggaaatat
     2341 cccgcaggta ccttcggtat tgatttccgt tacggctaat cacgcatccc acaggcccgc
     2401 acatcgggcc tgatgttctg tttcgagagt ccaaaatgaa agtgacatta acgtttaacg
     2461 aacaacgtcg tgcggcgtat cgtcagcaag ggctatgggg cgatgcttcg ctggccgatt
     2521 actggcagca gaccgctcgt gcgatgccag acaaaattgc cgtggtcgat aatcatggag
     2581 catcgtacac ctatagcgca ctcgatcaca ccgcgagctg tctggcaaac tggatgttag
     2641 cgaagggtat tgaatcaggc gatcgcatcg catttcaact gccaggctgg tgtgaattta
     2701 ccgttatcta tcttgcctgc ctgaaaatcg gtgcggtttc cgtaccgctg ttgccttcct
     2761 ggcgggaagc agaactggtg tgggtgctta ataagtgtca ggcaaaaatg ttctttgcac
     2821 cgacgttgtt taaacaaacg cgtccggtag atttaatcct gccgctgcaa aatcagcttc
     2881 cacaactaca acaaattgtc ggcgtggaca aactggctcc cgccacctct tccctctcat
     2941 taagtcagat tatcgccgac aataccccac tgaccacggc gataacgacc cacggcgatg
     3001 aattagctgc ggtgctgttt acctccggaa ccgagggtct gccaaagggc gtgatgctaa
     3061 cgcataacaa tattctcgcc agtgagcggg cttattgcgc gcggctgaat ctgacctggc
     3121 aggatgtctt tatgatgcct gcgccactcg gccacgcaac gggctttctg catggcgtaa
     3181 cagcaccatt tttgattggc gctcgcagcg tgttgttaga tattttcact cctgatgcgt
     3241 gtctcgcgct gcttgagcag cagcgttgca cctgtatgct cggcgcaacg ccgtttgtct
     3301 atgatctttt gaatttacta gagaaacaac ccgcagacct ttcagcgctg cgcttctttc
     3361 tttgcggcgg taccacaatc cccaaaaaag tggcacgtga atgccagcag cgcggcatta
     3421 aattattaag tgtttatggt tccacagaaa gttcgccgca tgcggtggtg aatctcgatg
     3481 atcctttgtc gcgctttatg cacaccgatg gttacgctgc cgcaggtgta gagattaaag
     3541 tggtcgatga cgcacgcaag accttaccgc caggttgcga aggtgaagaa gcctcgcgtg
     3601 gccccaatgt gtttatggga tattttgatg aacctgaatt aaccgcccgt gcgctggatg
     3661 aagaaggctg gtattacagc ggcgatctct gccgtatgga tgaggctggc tatataaaaa
     3721 ttaccggacg caaaaaagat attattgtcc gcggcggcga aaatattagc agccgtgaag
     3781 tggaagatat tttattgcag catcctaaaa ttcacgatgc ctgtgtggtt gcaatgcccg
     3841 atgaacgttt aggtgaacga tcatgcgctt atgtcgtgct gaaagcgccg catcattcat
     3901 tatcgctgga agaggtggtg gcttttttta gccgtaaacg ggtcgcaaaa tataaatatc
     3961 ctgaacatat cgtggtaatc gaaaaactac cgcgcactgc ctcaggtaaa atacaaaagt
     4021 ttttgttaag aaaagatatt atgcggcggt taactcagga tgtctgtgaa gagattgaat
     4081 aagtttcatc ttcggggatc acataaaccc ggcgacaaaa cgccgccggg tatttatttt
     4141 atttcttcag ttcagccagg cttaaccagg tttgcaccac ggtgtccgga ttcagagaca
     4201 ggctatcgat cccctcttcc atcaaccatg cggcaaagtc ttcgtggtcg gacggaccct
     4261 gaccgcaaat cccgacatat ttgccctgtt tcttcgcggc acgaatcgcc atcgacaaca
     4321 gtgctttcac cgcatcgttg cgctcatcga acaattcaga caccacgccg gagtcacggt
     4381 ccagaccgag cgccagctgc gtcatatcgt ttgagccaat tgagaagccg tcgaaatatt
     4441 cgaggaactg ctcggccagc aaggcgttgg acgggatttc acacatcatg atgattttca
     4501 gcccgttctc accacgcttc agcccctgac gcgccagttc ctcaaccacc gctttcgcct
     4561 gatctacggt acgcacgaac gggatcatga tctcaacgtt ggtcagcccc atgtcgttgc
     4621 gcacacgttt cactgcttcg cactccagcg cgaaacagtc gcggaagctg tcggaaacat
     4681 agcgtcccgc gccacggaag ccgagcatcg ggttctcttc atctggctcg taacgctcac
     4741 caccgaccag gttggcatat tcgttcgatt taaaatcaga gagacggaca atgacgcgct
     4801 tcggataaaa cgcggcaccc agcgtcgcga tcccttcagt cagacgacca acgtaaaatt
     4861 cacgcggaga atcaaaacct ttcatcatct cgcggatttc gttttgcaac tgcggttcct
     4921 gatcgtcaaa ctcaagcagt gcgcgtgggt ggacgccaat catacggttg atgataaatt
     4981 ccagacgcgc aagtcccacg ccttcgttcg gcagacaggc gaagtcgaaa gctcggtccg
     5041 ggttaccgac gttcatcatc actttcaacg gcagatccgg catcgtttct acgctggagc
     5101 ttttcacgct aaattccagc aactccgcat agacgtaacc ggtatcacct tcggcacaag
     5161 aaacagtgac gttctcaccg tctttcatcc gttctgttgc atcaccacag cccactaccg
     5221 ccggaatgcc cagttcacga gcgatgatcg ccgcgtgaca ggtacgaccg ccacggttgg
     5281 tgacgatggc agatgctttc ttcatgatcg gttcccagtc cgggtcggtc atgtcagtga
     5341 ccagcacgtc accaggttcg atgcggttca tttcgctgat atcatggatg actttcaccg
     5401 gacccgcacc gatgcgatga ccgatagcac ggccttcggc gataatctta ccctgtgaat
     5461 gcagcgtata acgctccatg acctgaccgc gtgagcgcac ggtttccgga cgcgcctgca
     5521 caatgaacag tttaccggtg tggccatctt tcgcccactc aatatccatc gggcgaccgt
     5581 agtgtttctc aatttgtacg gcctgttttg ccagttcctg cacttcttcg ttggtcagcg
     5641 agaagatgtc acgctgttcc tgcggtacgt cttcgatttt aacctgcttg ccgtgctcct
     5701 gggtcggcgc gtaaaccatg cggatttttt tcgaccccat ggtgcggcgc acgatagccg
     5761 ggcgattcgc tgcaagtgtc ggtttatgga cgtaaaactc atccgggtta accgcgccct
     5821 gcacgaccat ttcaccaagg ccccatgcgg aagtgataaa caccacctgg tcaaagccgg
     5881 attcggtatc aatggagaac atcacgccag aagatgcgag gtcagagcgc accatccgtt
     5941 gaacaccggc ggagagcgcc acaccacggt gatcgtaacc ctggtgcaca cgataagaga
     6001 tggcgcgatc gttaaacaga gaagcaaata catgtttcac tgccacgaga acggcgtcaa
     6061 aaccctgaac gttgaggaag gtttcctgct gaccggcaaa ggaagcgtcc ggcatatctt
     6121 ctgcagtagc ggaggagcgc accgcaaaag aggcgttttc gtcatcggcg gaaagctgtg
     6181 cataagcttc gcggatggca ttttccagct caggctggaa gggagtgtcg ataatccact
     6241 ggcggatttg cgcgcccgct ttcgcaagct gggtaacatc gtcaatatcc gttttatcca
     6301 gcagttcata aatgcgctgg tttacgccgc tttggtccag aaactggtta aacgcgtcgg
     6361 cggttgtggc gaaaccattc ggaacggaaa cacccattcc ggaaaggtta gtaatcattt
     6421 cacccaggga ggcatttttg cccccaaccc tgtctacatc attcatgccg agttggttat
     6481 accaaagcac cagcggtgac gagccattgt tggacatcga acaatccttt tgtgataaat
     6541 gaacggtttg agaaacacat ttctgcgcat ttatctttgc atatttaacc ggatgaaaaa
     6601 aacggtgaat cgttcaagca aatatatttt tttacttttt aagactgatc ccagcgttgc
     6661 gcaaatctgc tctcttcgac aatttccaca taaaccatcg gtataaacga aaccataaaa
     6721 atgaaatgct gttttcataa aaaataaaat tgaaggttca ttttataaac cagacataac
     6781 gtcctacgct tctgtgcgtt tttaatttat gctttcatag aattatgtct gcatcacggg
     6841 aagaacaaaa tggataatgc tgttgatcgc cacgtttttt atatttctga tggtacggca
     6901 ataactgcgg aggtattagg acacgcagta atgtcacaat ttcccgtcac tatcagcagc
     6961 attacgctgc cgtttgtcga aaatgagagc cgtgcacggg cagtgaagga tcagattgac
     7021 gcaatttatc accagacagg cgtgcgcccg ctggtcttct actccatcgt gttgccggag
     7081 attcgcgcca tcatcttgca aagtgaaggc ttttgccagg atatcgttca ggcgctggtt
     7141 gccccgctac aacaagagat gaaactggat ccaacgccga ttgctcatcg tacccatggc
     7201 cttaacccta ataatctcaa taaatatgat gcgcgcattg cggcgattga ttacaccctc
     7261 gcccacgatg acggcatttc gttgcgcaat ctggatcagg ctcaggtgat cctgctcggt
     7321 gtttctcgct gtggtaaaac ccccaccagt ctgtatctgg caatgcaatt tggtatccgc
     7381 gcggcaaact acccctttat tgccgacgat atggataatc tggtgctacc cgcgtcgctc
     7441 aaaccgcttc agcataaatt gttcggcctg actatcgacc cggaacgtct ggcggcgatt
     7501 cgcgaggaac gtcgggagaa cagccgctat gcctcgcttc gtcagtgcag gatggaagtc
     7561 gcggaagtag aagcgttgta ccgtaaaaat cagatcccgt ggattaacag taccaattat
     7621 tcggtagaag agattgccac caagatcctc gatatcatgg ggcttagccg ccgaatgtac
     7681 tagagaacta gtgcattagc ttattttttt gttatcatgc taaccacccg gcgaggtgtg
     7741 acacacctcg cacttgaaat cagctgcgat tggtttatcg tgatgcgcat cacttcccgg
     7801 tagtcctgcc gtagaagcaa caaatttctg agacttgtaa tgaacagaac tgacgaactc
     7861 cgtactgcgc gtattgagag cctggtaacg cccgccgaac tcgcgctacg gtatcccgta
     7921 acgcctggcg tcgccaccca tgtcaccgac tcccgccgca gaattgaaaa aatactgaat
     7981 ggtgaagata agcgactgtt ggtcattatt ggcccctgct cgatccacga tctcaccgct
     8041 gcaatggagt acgccacccg tctgcagtcg ctgcgcaacc agtaccagtc acggctggaa
     8101 atcgtaatgc gcacctattt tgaaaaacca cgaactgttg tcggctggaa agggctaatc
     8161 tccgatccgg atttaaacgg cagctatcgg gtaaatcacg gtctggagct ggcgcgcaaa
     8221 ttacttttac aggtaaatga gctgggtgtc cctaccgcga ctgagtttct cgatatggtg
     8281 accggtcagt ttattgctga tttaatcagt tggggcgcga ttggcgcacg tactaccgaa
     8341 agtcagatcc accgcgaaat ggcttcagca ctctcctgtc cggtaggttt taaaaatggt
     8401 accgatggca acacgcggat tgccgtggat gctatccgcg cagcccgcgc cagccatatg
     8461 ttcctctcgc cagacaaaaa tggtcagatg accatctatc agaccagcgg caacccgtat
     8521 ggccacatta ttatgcgtgg cggcaaaaaa ccgaattatc atgccgatga tatcgccgca
     8581 gcctgcgata cgctgcacga gtttgattta cctgaacatc tggtggtgga tttcagccac
     8641 ggtaactgcc agaagcagca ccgtcgccag ttagaagttt gtgaggatat ttgtcagcaa
     8701 atccgcaatg gctctacggc gattgctgga attatggcgg aaagtttcct gcgcgaagga
     8761 acgcaaaaaa tcgtcggcgg tcagccgctc acttacggtc aatccattac cgacccgtgt
     8821 ctgggctggg aggataccga acgcctggtc gaaaaactcg cctctgcggt agatacccgc
     8881 ttctgaatgc gtgcccattc ctgacggaat gggcatttct gcgcaacttg ttgtcttctc
     8941 aacaaattac tgcttgctct ggtcagccat aatattgata ataagaatca ttgttatatc
     9001 aattattatt aatttttatg cgttatacgg atagcagaaa actcacgcct gaaacggatg
     9061 ccaatcacaa gaccgcttcc ccgcagccta ttcggcgaat ttccagccag acactgttag
     9121 gtccggatgg caaactgatt atcgatcatg acgggcaaga atatctgctc cgtaaaactc
     9181 aggctggcaa gctgctgttg accaagtagc ctttaactcg agcagctgac ttccagccgt
     9241 ttaccccagt caggtggacg actgacatag tcatcatctc tgtcgctgaa aggatttcgc
     9301 aacgcctcat gcaggcggtg caattccgtc atatcaccct tttctgccgc ttcaatcgcc
     9361 cgttgcgcca accagttgcg caataccaga gcggggttaa cgctttgcat cagttgctga
     9421 cgctcactat cgctaacctc gtcttgttgc aaacgccccc gataacgggc aaaccagtca
     9481 tcaaatgccg cacgatcaat aaactcatca cgtagcggtg acgccgcgct gtgctgctcg
     9541 gtcagactca gcatgcggaa tgtgcgggta taatcgctgc gctctcgcgc catcagactg
     9601 aataattcat tcagtagcgc gttatcctct ttttgctccg tcatgaagcc cagtttctgc
     9661 cgcatccgtt ctccataatg cgtcaacaaa acctgctgat agctgtccag ggcctcattc
     9721 agggcatcta cggcaacaaa tggcgacaat gtctgcgcca gacgctgtaa attccacaac
     9781 gcgacggcag gttgattatc aaagctgtaa cgcccttgat gatccgagtg attacaaata
     9841 aaaccgggtt cgtaatcatc aagaaaacca aacggcccgt aatcaagcgt cagccccagc
     9901 agcgacatgt tgtcggtatt catcacccca tgagcaaagc cgaccgtctg ccattgggca
     9961 attaacgacg cggtacgtgc gacaacatcg ctaaaccaga gacggtattt gtcctcatca
    10021 tcttcaagat gtgaccagta atgacgaata gcaaagtcag ctaactgacg aaccttttcc
    10081 gactcgcggc ggtagtaaaa atgttcgaaa tgaccaaagc gcagatgact tggtgccaca
    10141 cgcatcagca tcgcgcctgg ctccgccgtt tcccgataca ctggcgaatc gctggtgacg
    10201 atacttaacg cgcgggtcgt cggaatgccc agataatgca tcgcctcact ggcgagactt
    10261 tctcgtatcg tcgaacgtaa aaccgcccgt ccatcaccca ttcgcgaata aggcgtcagg
    10321 ccagcacctt tcagatgcca gtccattgta gtgccatcag caagcagttg ttcgccgagt
    10381 aaaatgccgc gcccatcacc cagttggccc gcccagacgc cgaactgatg accactgtaa
    10441 acctgcgcca gtggtgacat accaggcagt agggcttcgc cgccccagac acctgcgcca
    10501 tttttaaaca gcgacgatgg aatactcagc gtgttagcca gttcggtatt atgccaaatc
    10561 agccgggcat tatttaaagg cgtaggggaa agtgctgtat aggtttctgg caattcatcg
    10621 cgccagcggg taacaaaaga cagggtcata gatcctcctg tttgatagtg tagacggtta
    10681 ctctcgtctt aaacaccagc aaacagaagg gttatcgctg aaccaacgtc gttatctgat
    10741 ttatgggaac ggcaggccac aggcatccct ggagagcatg gaaatcgaag ggggtaatct
    10801 gtgccaatat ttctgccgta tcaatgcctc ccgcaatgat gcaattgcaa caaggggaaa
    10861 tttgcgcctg gatcgcacga ataaacggtt cgaaggagcg gtgcgtgatt tgttgctgaa
    10921 taaaactttt atccagcatg acgcgggtga aaagaccatc aaaaaccgct ttcattgtgc
    10981 tattgcctgc tcccaaatta cctaacacaa ggggatacac ttgcgataag gataaaagac
    11041 cgcgattatc ttttccttca ttgagatgcg ggtaattctc attaatcaac agttcaataa
    11101 acggatattt taatagttcg cctgcgtaat tatccctctc taataataat gtagccactt
    11161 gtggcgttaa attaagtcag gcaaagagtt tatgttgaat aaaaaaatgt tggcatgatt
    11221 ttagtaattc caa
//
LOCUS       DACAPB010000149        11093 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.149, whole genome shotgun sequence.
ACCESSION   DACAPB010000149 DACAPB010000000
VERSION     DACAPB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.149"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            63..2084
                     /locus_tag="IT904_003511"
     CDS             63..2084
                     /locus_tag="IT904_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase mngB"
                     /protein_id="HAP3193246.1"
                     /translation="MLEKASVTKEILLPNGHDQMPLQQNIFEVMDKLREIYPQRKFVM
                     SRFEEVFEKIEAQRDNLATLKGEFIDGKYMRVHRTIGSTRMDIKIAHARIENKIVNLL
                     EPLATLAWTLGFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMA
                     DNLLRFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDCGQPV
                     PYFIRHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVI
                     AAKSDAEGILENAFWQIALNEDGSLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAK
                     EEWVITAANAKPQCDIIHEAWQSRAVIRYDMAVPLNLSERSARQSTGRVGGVLVVTLS
                     HNSRRIDVDINLDNQADDHRLRVLVPTPFNTDSVLADTQFGSLTRPVNDSAMNNWQQE
                     GWKEAPVPVWNMLNYVALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGK
                     EDLLLRPGRPSGIKMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCY
                     NKIPWDAMKLNKAGFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATC
                     DATVAFSREVISCSETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            2931..4499
                     /gene="cydA"
                     /locus_tag="IT904_003512"
     CDS             2931..4499
                     /gene="cydA"
                     /locus_tag="IT904_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAP3193247.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            4515..5654
                     /gene="cydB"
                     /locus_tag="IT904_003513"
     CDS             4515..5654
                     /gene="cydB"
                     /locus_tag="IT904_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP3193248.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            5669..5782
                     /gene="cydX"
                     /locus_tag="IT904_003514"
     CDS             5669..5782
                     /gene="cydX"
                     /locus_tag="IT904_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAP3193249.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            5782..6075
                     /gene="ybgE"
                     /locus_tag="IT904_003515"
     CDS             5782..6075
                     /gene="ybgE"
                     /locus_tag="IT904_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAP3193250.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            6225..6629
                     /gene="ybgC"
                     /locus_tag="IT904_003516"
     CDS             6225..6629
                     /gene="ybgC"
                     /locus_tag="IT904_003516"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAP3193251.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            6626..7318
                     /gene="tolQ"
                     /locus_tag="IT904_003517"
     CDS             6626..7318
                     /gene="tolQ"
                     /locus_tag="IT904_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAP3193252.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            7322..7750
                     /gene="tolR"
                     /locus_tag="IT904_003518"
     CDS             7322..7750
                     /gene="tolR"
                     /locus_tag="IT904_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAP3193253.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            7815..9080
                     /gene="tolA"
                     /locus_tag="IT904_003519"
     CDS             7815..9080
                     /gene="tolA"
                     /locus_tag="IT904_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAP3193254.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAVALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            9213..10505
                     /gene="tolB"
                     /locus_tag="IT904_003520"
     CDS             9213..10505
                     /gene="tolB"
                     /locus_tag="IT904_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAP3193255.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGLQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            10540..11061
                     /gene="pal"
                     /locus_tag="IT904_003521"
     CDS             10540..11061
                     /gene="pal"
                     /locus_tag="IT904_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAP3193256.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
BASE COUNT         2749 a         2578 c         2972 g         2794 t
ORIGIN      
        1 tcggtaagta cttacctgcc gacgaaaacg gattacgtaa acgcctcgac agttattttg
       61 acgtgctgga aaaagcgtct gtaaccaaag agattttgct gccgaatggg catgaccaga
      121 tgccattgca gcaaaatatc ttcgaagtga tggataagct acgtgagatc taccctcaac
      181 gtaagtttgt gatgagccgc tttgaagagg tatttgagaa gatcgaagcg cagcgagata
      241 atctggcaac cctgaaaggg gaatttattg atggcaaata tatgcgcgtg catcgcacca
      301 tcggttctac gcgtatggat atcaaaattg cccacgcgcg tattgaaaat aagattgtta
      361 atctgctgga accgctggca acgctggcct ggacgttggg ttttgaatac caccacggct
      421 tgctggagaa aatgtggaaa gagatcttaa aaaatcatgc ccacgacagt atcggttgtt
      481 gttgcagtga caaagttcat cgcgaaattg tcgcccgctt cgaactggct gaagacatgg
      541 cggataatct gctgcgtttc tacatgcgca aaattgccga taacatgcca cagagtgatg
      601 ccgacaaact cgtgctgttt aacctgatgc cctggccgcg ggaagaagtt attaacacca
      661 ctgtgcggct gcgcgccagc cagtttaatc tacgagacga ttgcggtcag cctgtaccgt
      721 attttattcg ccatgcccgt gagatcgatc ctggcctaat cgatcggcaa attgttcatt
      781 atggcaatta tgatcccttt atggagtttg atattcagat caaccagatt gtcccttcta
      841 tgggctatcg cacgctttat attgaagcga atcagcctgg caacgtgatt gcggcaaaaa
      901 gtgacgctga agggatactg gaaaatgctt tctggcaaat tgcgctcaat gaggatggtt
      961 ctctgcaact ggtagataaa gacagcggtg tgcgctatga ccgggtattg caaattgaag
     1021 aaagctctga tgatggtgat gaatatgact attcacccgc aaaagaagag tgggtaatta
     1081 ccgcagcgaa cgcgaaaccg caatgcgata ttattcatga agcctggcag agcagggctg
     1141 ttatccgcta tgacatggca gtgccgctca atttgtcaga acgcagcgcc cggcaatcca
     1201 ctggcagagt agggggggtg ttggttgtca ctcttagtca taacagcagg cgtattgatg
     1261 tggatatcaa ccttgataac caggctgacg atcatcgcct tcgtgtcctg gtccctacac
     1321 cttttaacac cgacagtgtt ctggcagata cgcagtttgg ttcgctaacg cgccccgtga
     1381 acgacagtgc aatgaacaac tggcagcaag aaggctggaa agaagcgccg gttccggtat
     1441 ggaatatgct caactatgtt gccttacagg aagggcgtaa cggcatggct gtctttagcg
     1501 aagggttacg tgaatttgaa gtcatcggtg aagagaagaa aacctttgcc attacgttgc
     1561 tgcgtggcgt gggcttactg ggcaaagaag atctgctttt aaggcctggg cggccttcgg
     1621 gaattaaaat gccagtcccg gactcacaac tacgtggtct gctttcttgt cgcctaagtt
     1681 tattgagtta taccggtacg ccaaccgccg ctggtgtagc tcagcaggcg cgagcatggc
     1741 tgactccagt acagtgttac aacaaaatcc catgggatgc gatgaagctc aacaaagccg
     1801 gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc cccagtggga tgcctgataa
     1861 gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt acggctgttt aatccggctg
     1921 aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga ggtgatttct tgctcagaaa
     1981 cgatgatgga tgaacacatt accaccgagg aaaatcaagg ttcaaatcta tcggggcctt
     2041 ttttacccgg ccagtcacgg acgttcagtt accggcttgc ctgaatagca atcaaaccga
     2101 agccacatat gcgcggccag attgttgaca aagggcgctt tgttcatgcc ggatacggca
     2161 tgaacgcttt attcggtcta caaaagcagg caaattcaat atattgcaga gatgatgtag
     2221 acactggcaa gcgtagcgca tcaggtaatt ttgcgtttat cttcactctc aagccacgta
     2281 tatgtggctt tatttttaac aaaataataa ccctgggtga gttaattata atataattat
     2341 aagttaatta aatgttaata ttggcggggt ggatttatgc ctttattagt aatcctgaaa
     2401 ctctgcgtcg tattagccag tgaccaaaaa aagaattaag gtcaaccgtg ctgtttttgc
     2461 ttcgtctctt tttatcttta attgccaacc gaaactaatt tcagccttat aactcacaca
     2521 ttttaaacat aaatgtcact aaagttacct tattgaaaca tgattaacat aatttgtagg
     2581 aattgatatt tatcaatgta taagtcttgg aaatgggcat caaaaagaga taaattgttc
     2641 tcgatcaaat tggctgaaag gcggtaattt agctataaat tgatcaccgt cgaaaaatgc
     2701 aaatttgctt caacaaaaac ctgtttattg taaggatttt gcggcgtaat atatacgtgg
     2761 gatcaatttg agtttttatt aacatgtttg caacctttct ttacgccgtt tttgtgtgca
     2821 ttcacatggt atgatgaaag tgttcaaaca aatttctatt ggggcatgcg tgtgaccctt
     2881 tctaacgggg ttcactctcg gagtcttcat gcgatgagca aggagtcatg atgttagata
     2941 tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat gtaccacttc ctttttgtgc
     3001 cactgacgct cggtatggcg ttcctgctgg ccattatgga aacggtctac gtcctctccg
     3061 gcaaacagat ttataaagat atgaccaagt tctggggcaa gttgtttggt atcaacttcg
     3121 ctctgggtgt ggctaccggt ctgaccatgg agttccagtt cgggactaac tggtcttact
     3181 attcccacta tgtaggggat atcttcggtg cgccgctggc aatcgaaggt ctgatggcct
     3241 tcttcctcga atccaccttt gtaggtctgt tcttcttcgg ttgggatcgt ctgggtaaag
     3301 ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc aaacctgtcc gcactgtgga
     3361 ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc cgatttcaac tttgaaacta
     3421 tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa cccggttgct caggtgaaat
     3481 tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat gttcatcctc ggtatcagcg
     3541 catggtatat gctgaaaggt cgtgacttcg ccttcgctaa acgctccttt gctatcgctg
     3601 ccagcttcgg tatggctgct gttctgtctg ttattgttct gggtgatgaa tccggctacg
     3661 aaatgggcga cgtgcagaaa accaaactgg ctgctattga agccgagtgg gaaacgcaac
     3721 ctgcgcctgc tgcctttact ctgttcggca ttcctgatca ggaagaggag acgaacaaat
     3781 ttgcgattca gatcccttac gcactgggca tcattgcaac gcgttccgtg gataccccgg
     3841 ttatcggcct gaaagagctg atggtgcagc atgaagaacg cattcgtaac gggatgaagg
     3901 cgtactctct gctcgaacaa ctgcgttctg gttctaccga ccaggcggtt cgtgaccagt
     3961 tcaatagcat gaagaaagac ctcggttacg gtctgctgct gaaacgctat acgccaaacg
     4021 tggctgatgc gactgaagcg cagattcaac aggcaaccaa agactccatc ccgcgtgtag
     4081 cgccgctgta ctttgcgttc cgtatcatgg tggcgtgtgg cttcctgctt ctggcaatca
     4141 tcgcgctctc tttctggagt gtcatccgca accgcattgg cgagaaaaaa tggcttctgc
     4201 gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt agaagcgggc tggttcgtgg
     4261 ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct gccgacagct gtggcgaact
     4321 cgtcactgac cgcaggcgat ctcatcttct caatggtgct gatttgcggc ctgtataccc
     4381 tgttcctggt ggcagaattg ttcttaatgt tcaagtttgc acgcctcggc ccaagcagcc
     4441 tgaaaaccgg tcgctatcac tttgagcagt cttccacgac tactcagccg gcacgctaag
     4501 acaggagtcg tcaaatgatc gattatgaag tattgcgttt tatctggtgg ctgctggttg
     4561 gcgttctgct gattggtttt gcagtcactg acggtttcga catgggggtg ggcatgctca
     4621 cccgtttcct cggtcgtaac gacaccgagc gtcgaattat gattaactcc attgcaccac
     4681 actgggacgg taaccaggtt tggctgatca ccgcgggcgg cgcactcttt gctgcctggc
     4741 cgatggtcta tgccgctgcg ttctccggct tctatgtggc gatgatcctc gtgctggcgt
     4801 ctttgttctt ccgtccggtc ggttttgact accgctccaa gattgaagaa acccgctggc
     4861 gtaacatgtg ggactggggc atcttcattg gtagcttcgt tccgccgctg gtaattggtg
     4921 tagcgttcgg taacctgttg cagggcgtac cgttcaacgt tgatgaatat ctgcgtctgt
     4981 actacaccgg taacttcttc cagttgctta acccgttcgg cctgctggca ggcgtggtga
     5041 gcgtagggat gatcattact cagggcgcaa cctatctgca aatgcgtacc gtgggcgaac
     5101 tgcacctgcg tacccgtgca acggctcagg tggctgcgct ggtgacactg gtctgtttcg
     5161 cactggctgg cgtatgggtg atgtacggta tcgatggtta tgtcgtgaaa tcgacaatgg
     5221 accattacgc agcctctaac ccactgaata aagaagtggt tcgtgaagct ggcgcatggc
     5281 tggttaactt caacaacacg ccaattctgt gggctattcc ggcactgggt gtggttctgc
     5341 cgctgctgac catcctgact gcacgtatgg ataaagccgc gtgggcgttt gtgttctcct
     5401 ccctgacgct ggcctgcatc atcctgacag ccggtatcgc aatgttcccg tttgtgatgc
     5461 cgtccagcac catgatgaac gcaagtctga caatgtggga tgcaacttcc agccagctga
     5521 cgcttaacgt catgacctgg gttgcggtgg ttctggtacc gatcattctg ctctacaccg
     5581 cctggtgtta ctggaaaatg ttcggtcgta tcaccaaaga agatattgaa cgtaacaccc
     5641 actctctgta ctaagtaagg agctaaaaat gtggtatttc gcatggattc tgggaacgct
     5701 tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt gaacacgtcg aatcaggcaa
     5761 agccggtcaa gaagacatct gatgagtaag attatcgcga ctttgtatgc ggtaatggac
     5821 aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc tgttagcagg atgtatgttt
     5881 tgggacccat ctcgttttgc cgcgaagacc agtgaactgg aaatctggca tggtttattg
     5941 ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg gctttcgtcc gcagaaggtt
     6001 ctttggcaag ggattttttg cccattgctt gccgatattg ttctcattgt cgggctgatt
     6061 ttcttcttct tttaaatcag aattctcttt aaaaaattat gggccgctcc aggcccataa
     6121 atttttacgc tcccttaact tgccctcatt cccaaacctc aatcgcgcgc gtatagtagc
     6181 agcgtttaaa agctctaact tttgttgcat taccgggatg taaagtgaat acaacgctgt
     6241 ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg gtgtaccacg
     6301 ccagttacgt cgctttttat gaaagagcac gcacagagat gctgcgtcat catcacttca
     6361 gtcagcaggc gctgatggct gaacgcgttg cctttgtggt acgtaaaatg acggtggaat
     6421 attacgcacc tgcgcggctc gacgatatgc tcgaaataca gactgaaata acatcaatgc
     6481 gtggcacctc tttggttttc acgcaacgta ttgtcaacgc cgagaatact ttgctgaatg
     6541 aagcagaggt tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt gcgcttccca
     6601 agtctattgt cgcggagttt aagcagtgac tgacatgaat atccttgatt tgttcctgaa
     6661 ggctagcctt ctggttaaac ttatcatgtt gattttgatt ggtttttcaa tcgcatcttg
     6721 ggccattatt atccagcgga cccgtattct taacgcagcg gcgcgcgaag ccgaagcgtt
     6781 tgaagataaa ttctggtctg gaatcgaact ctctcgcctc tatcaagaga gccaggggaa
     6841 acgggataat ctgactggtt cggaacaaat cttttacagc gggttcaaag agtttgtgcg
     6901 cctgcatcgt gccaatagcc atgcgccgga agccgtagtg gaaggggcgt cgcgtgctat
     6961 gcgtatctcc atgaaccgtg aacttgaaaa tctggaaacg cacattccgt tcctcggtac
     7021 ggttggctcc atcagcccgt atattggtct gtttggtacg gtctggggga tcatgcacgc
     7081 ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa atggttgcgc ccggtatcgc
     7141 agaagcgttg attgcgactg caattggtct gtttgccgct atcccggcag ttatggccta
     7201 caaccgcctc aaccagcgcg taaacaaact ggaactgaat tacgacaact ttatggaaga
     7261 gtttaccgcg attctgcacc gccaggcgtt taccgttagc gagagcaaca aggggtaagc
     7321 catggccaga gcgcgtggac gaggtcgtcg cgatctcaag tccgaaatca acattgtacc
     7381 gttgctggac gtactgctgg tgctgttgct gatctttatg gcgacagcgc ccatcatcac
     7441 ccagagcgtg gaggtcgatc tgccagacgc tactgaatca caggcggtga gcagtaacga
     7501 taatccgcca gtgattgttg aagtgtctgg tattggtcag tacaccgtgg tggttgagaa
     7561 agatcgcctg gagcgtttac caccagagca ggtggtggcg gaagtgtcca gccgtttcaa
     7621 ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt acgatgaaat
     7681 aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt taatgacgca
     7741 gcctatctaa acatctgcgt ttcccttgct tgaaagagag cgggtaacag gcgaacagtt
     7801 tttggaaacc gagagtgtca aaggcaaccg aacaaaacga caagctcaag cgggcgataa
     7861 ttatttcagc agtgctgcat gtcatcttat ttgcggcgct gatctggagt tcgttcgatg
     7921 agaatataga agcttcagcc ggaggcggcg gtggttcgtc catcgacgct gtcatggttg
     7981 attcaggtgc ggtagttgag cagtacaaac gcatgcaaag ccaggaatca agcgcgaagc
     8041 gttctgatga acagcgcaag atgaaggaac agcaggctgc tgaagaactg cgtgagaaac
     8101 aagcggctga acaggaacgc ctgaagcaac ttgagaaaga gcggttagcg gctcaggagc
     8161 agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag caagctgaag
     8221 aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcagat gctaaagctg
     8281 cggaagaagc agcgaagaaa gcggctgcag acgcaaagaa aaaagcagaa gcagaagccg
     8341 ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc cgctgtggca ctgaagaaga
     8401 aagcggaagc ggcagaagca gctgcagctg aagcaagaaa gaaagcggca actgaagctg
     8461 ctgaaaaagc caaagcagaa gctgagaaga aagcggctgc tgaaaaggct gcagctgata
     8521 agaaagcggc agcagagaaa gctgcagccg acaaaaaagc agcagaaaaa gcggctgctg
     8581 aaaaggcagc agctgataag aaagcagcgg cagaaaaagc cgccgcagac aaaaaagcgg
     8641 cagcggcaaa agctgcagct gaaaaagccg ctgcagcaaa agcggccgca gaggcagatg
     8701 atattttcgg tgagctaagc tctggtaaga atgcaccgaa aacgggggga ggggcgaaag
     8761 ggaacaatgc ttcgcctgcc gggagtggta atactaaaaa caatggcgca tcaggggccg
     8821 atatcaataa ctatgccggg cagattaaat ctgctatcga aagtaagttc tatgacgcat
     8881 cgtcctatgc aggcaaaacc tgtacgctgc gcataaaact ggcacccgat ggtatgttac
     8941 tggatatcaa acctgaaggt ggcgatcccg cactttgtca ggctgcgttg gcagcagcta
     9001 aacttgcgaa gatcccgaaa ccaccaagcc aggcagtata tgaagtgttc aaaaacgcgc
     9061 cattggactt caaaccgtaa tcgcgatgtt gactgttcgg acggtcaaca tcaggcaccg
     9121 gttgccacgg ggttctggta gttttgtgta ttttagtttg ttaacattct gctaaattat
     9181 cgtgggccat cggtccagat aagggagata tgatgaagca ggcattacga gtagcatttg
     9241 gttttctcat actgtgggca tcagttctgc atgctgaagt ccgcattgtg atcgacagcg
     9301 gtgtagattc cggtcgtcct attggtgttg ttcctttcca gtgggcgggg cctggtgcgg
     9361 cacctgaaga tattggcggc atcgttgctg ctgacttgcg taacagcggt aaatttaatc
     9421 cgttagatcg cgctcgtctg ccacagcagc cgggtagtgc gcaggaagta caaccagctg
     9481 catggtccgc actgggcatt gacgctgtag ttgtcggtca ggtcactccg aatccggatg
     9541 gttcttacaa tgttgcttat caacttgttg acactggcgg cgcaccgggt actgtacttg
     9601 ctcagaactc gtacaaagtg aacaagcagt ggctgcgtta tgctggtcat accgccagtg
     9661 atgaagtgtt tgaaaaactg accggcatta aaggtgcgtt ccgtactcgt attgcctacg
     9721 ttgttcagac caacggcggt cagttcccgt atgaactgcg cgtatctgac tatgacggtt
     9781 acaaccagtt tgtcgttcac cgttcaccgc agccgctgat gtctccggcg tggtcaccag
     9841 acggttctaa actggcttat gtgaccttcg aaagcggtcg ttccgcgctg gttattcaga
     9901 cgctggcaaa tggcgctgta cgtcaggtgg cttcattccc gcgtcacaac ggtgcacctg
     9961 cattctcgcc agacggcagc aaactggcat tcgccttgtc gaaaaccggt agtctgaacc
    10021 tgtacgtaat ggatttggct tctggtcaga tccgccaggt gactgatggt cgcagtaaca
    10081 ataccgaacc gacctggttc ccggatagcc agaacctggc atttacttct gaccaggccg
    10141 gtcgtccgca ggtttataaa gtgaatatca acggcggtgc gccacaacgt attacctggg
    10201 aaggtttgca gaaccaggat gcggatgtca gcagcgacgg taaatttatg gtaatggtca
    10261 gctccaatgg tgggcagcag cacattgcca aacaagatct ggcaacggga ggcgtacaag
    10321 ttctgtcgtc cacgttcctg gatgaaacgc caagtctggc acctaacggc actatggtaa
    10381 tctacagctc ttctcagggg atgggatccg tgctgaattt ggtttctaca gatgggcgtt
    10441 tcaaagcgcg tcttccggca actgatggac aggtcaaatt ccctgcctgg tcgccgtatc
    10501 tgtgataata attaattgaa tagtaaagga atcattgaaa tgcaactgaa caaagtgctg
    10561 aaagggctga tgattgctct gcctgttatg gcaattgcgg catgttcttc caacaagaac
    10621 gccagcaatg acggcagcga aggcatgctg ggtgccggca ctggtatgga tgcgaacggc
    10681 ggcaacggca acatgtcttc cgaagagcag gctcgtctgc aaatgcaaca gctgcagcag
    10741 aacaacatcg tttacttcga tctggacaag tacgatatcc gttctgactt cgctcaaatg
    10801 ctggatgcac atgcaaactt cctgcgtagc aacccgtctt acaaagtcac cgtagaaggt
    10861 cacgcggacg aacgtggtac tccggaatac aacatctccc tgggtgaacg tcgtgcgaac
    10921 gccgttaaga tgtacctgca gggtaaaggc gtttctgcag accagatctc catcgtttct
    10981 tacggtaaag aaaaacctgc agtactgggt catgacgaag cggcatactc caaaaaccgt
    11041 cgtgcggtac tggtttacta agagaattgc atgagcagta acttcagaca tca
//
LOCUS       DACAPB010000150        10944 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.150, whole genome shotgun sequence.
ACCESSION   DACAPB010000150 DACAPB010000000
VERSION     DACAPB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.150"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(1..793)
                     /locus_tag="IT904_003522"
                     /pseudo
     CDS             complement(1..793)
                     /locus_tag="IT904_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
     gene            1169..2809
                     /gene="trg"
                     /locus_tag="IT904_003523"
     CDS             1169..2809
                     /gene="trg"
                     /locus_tag="IT904_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAP3193257.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANKLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEIDKVTQQNASLVEEASAAALS
                     LEEQAARLTEAVDVFRLHKHSVSAKPRGAGEPVSFATV"
     gene            complement(2847..3770)
                     /locus_tag="IT904_003524"
     CDS             complement(2847..3770)
                     /locus_tag="IT904_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3193258.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3987..5330
                     /locus_tag="IT904_003525"
     CDS             3987..5330
                     /locus_tag="IT904_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP3193259.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     LLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5555..7210
                     /locus_tag="IT904_003526"
     CDS             5555..7210
                     /locus_tag="IT904_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAP3193260.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7350..7574
                     /locus_tag="IT904_003527"
     CDS             7350..7574
                     /locus_tag="IT904_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAP3193261.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7637..8173
                     /gene="rimL"
                     /locus_tag="IT904_003528"
     CDS             7637..8173
                     /gene="rimL"
                     /locus_tag="IT904_003528"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAP3193262.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIGS"
     gene            complement(8168..9148)
                     /locus_tag="IT904_003529"
     CDS             complement(8168..9148)
                     /locus_tag="IT904_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAP3193263.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9272..10264
                     /gene="tehA"
                     /locus_tag="IT904_003530"
     CDS             9272..10264
                     /gene="tehA"
                     /locus_tag="IT904_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAP3193264.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10261..10854
                     /gene="tehB"
                     /locus_tag="IT904_003531"
     CDS             10261..10854
                     /gene="tehB"
                     /locus_tag="IT904_003531"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAP3193265.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVMEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDSADYPCTVGFPFAFKERELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         2771 a         2585 c         2764 g         2824 t
ORIGIN      
        1 atcgctgctt taagtgagag ggttcccatc aggcgctgaa cagctttatg gttaatctgt
       61 ttcccttctc gatgaagaga cagcgttacc ctacggtatc cgtatcggcc tctattctcg
      121 tgataaatct cactaatacg ctttttaacg tccgcatact tgtcaggctt gctgagagcc
      181 tttagatgat aataaaacgt actgcgcggt atctccgcag ccctgagaag ctcatcaaga
      241 ggataaaact gccttagctc gttgagtact ttcacttttt cgtgggatga gctaaggctt
      301 tcagcttttt tagatacata agccgcgttt caagaaatcg aacttgcctt tcaagatcct
      361 caatgcgtcg gtcttttgac agctccaatg ctgatgccgc tttttctgga tcaactgata
      421 ttgcaatgtt tcttttggtg ccaatcttga gcgcgcgtaa accagcttct ccgcgctctt
      481 catagacctt cagccacctg gctacagaac cactaccagc aagcataaag tgagcagcag
      541 cctgattaag ggacatgtgc tgctcgatca cagctttcac gaccttaata cgcaactctg
      601 gatcagcact aacgccttta ggtttgggaa ttaaaccttt ttctccatgt ttttcataga
      661 gggcaaccca tgtcctgacc tgggttcggg ggacaccaaa acgtgccgag atgatcctgt
      721 aaccatcatc agttgtgaag tagtgattca cgacttcaag gcgcttttca aaagggtatt
      781 ttggctttga catattaggg gctattccat ttcatcgtcc aacaaaatgg gtgcagtaca
      841 gttgatagaa atatcggtcg gggccttcgt ctttctgatt cccggttagc ctgaaaacag
      901 aaagtctcag gcacccgcag gcatcctatg aggtttcctt agggacgaaa ataatcactt
      961 cacgaaattg cgtgctgttt tccagaattt ttcgtcattc gggttagccc gtttaatcat
     1021 tcgttactct cttcattcca atggcattaa ccttctctgc aataattgtt gtaaaaatgt
     1081 gacgcaaaga ggcttttagt cgtaagtaat taccgtcaag tgccgatgac tttctatcag
     1141 gagtaaacct ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt
     1201 tttgcatcac atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct
     1261 attcgcatta agttctgctc tggctggcta tttcctctgg caggccgatc gcgatcagcg
     1321 tgatgttact gcggagattg agattcgaac cgggttagcg aacagttcag attttttgcg
     1381 ttcagcccgg atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc
     1441 aatgaagcga aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg
     1501 tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa
     1561 tcaacgcttt caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg
     1621 catgtttgaa gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta
     1681 taccgatatt ttgaacaaag ccgttaagat acgtagcacc agagccaaca aactggcgga
     1741 actggcccat cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcttgc
     1801 cctggtcatg acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca
     1861 acatgccgca caacggattg aaaaaatcgc tagtggcgat ctgacgatga aggatgaacc
     1921 ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact
     1981 ggggatgaca gtagggactg ttcgacaggg tgcggaagag atttatcgtg gcaccagcga
     2041 aatttcagct ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga
     2101 acaaactgcc gctagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc
     2161 gcatcatgcc agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac
     2221 ggtttccggt gtagtaaaaa cgatgggcgc tatctctaca agttcgaaga aaatttccga
     2281 gatcaccgcc gtcatcaaca gtattgcttt ccagacgaat attctggcac tgaatgctgc
     2341 cgttgaagcc gcgcgagcgg gtgagcaagg ccgtggattt gccgttgtcg ccagcgaagt
     2401 acggacactc gcaagccgca gcgcccaagc ggcgaaagag attgaaggct tgatcagtga
     2461 atcagtcagg ttaattgacc tggggtcgga tgaggtggca acggcaggga aaaccatgag
     2521 cactattgtt gatgccgtcg cgagtgtcac acatatcatg caggaaatcg ccgccgcctc
     2581 ggatgaacaa agtagaggca taacgcaggt tagccaggcg atttctgaaa tagataaggt
     2641 gacgcaacag aatgcttctc tggtagaaga ggcctcagcg gcggcgttgt cccttgaaga
     2701 acaggcggca cgattaactg aggcggtgga tgtattccgt ctgcacaaac attctgtgtc
     2761 ggcaaaacct cgcggagcgg gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag
     2821 gagggatcga cagatccctt cacctttcag aacggcattg attttcgaat agcgttaatc
     2881 atcaactggc aaccagaaga gaacgtcgca tctacgcggg tcagtattcc aatcggttcg
     2941 cctgcaccat gtcccggaac aggcagggcc accagcgtgg catgacgcag gtcgtctttt
     3001 accgcgccag aagggacaaa ccacacgtag tcgtattcaa ccgtaagttg acgagatagc
     3061 gaagcagaca gcgtttcgat acaacccgaa ggaattttac atccctggct ctgtactaat
     3121 gcatctgaat gctggcgtgg cgcagtgcct tctggtgata caacgaccgg ccattccagc
     3181 acccggctta gcgttacgtt ctcctgaagt agcgggtgat tagggcggac aaccagcttc
     3241 agcgattcaa gaaacagcag ttcgtaatta agcccggtca tcagttcagg atctgacatc
     3301 cgaccaatgc cgatatcgat ttccccggtt ttcaaacccg ccagaatcat agggttactc
     3361 attgtcgcaa cttgcaaggt cgtttctttt tgttgctgat gaaactgacc tataaccgaa
     3421 ggtaatatcc ccagtgccgc agtaggtagt gcaccaaccc tgacgacatc attattaagg
     3481 ccttctttac gatgaagcga ctgtccggca gtgttgatgg cgtcaagaac tctgactgca
     3541 tgcgttaaaa attgttcgcc gggtaaggta agttgcgccc cctgacgacc acgctcaaac
     3601 aagcgagcgc ccgtcagctg ctccagttca ttcaatgtct tagagagcgc aggttgactc
     3661 aaattaaggg tttcagccgc gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta
     3721 tgaaggtggc gcaaacggat gcgctgacta aacagactat ttttttccat aagcgatgtt
     3781 aaaaacgaag cggtgtcgct gacaagtgaa gttgtttgat tatgataact tgattgcaaa
     3841 atattattaa caattaaagc aatcatgtta cagcaaaatg gataatattg atgttttcgc
     3901 ggcgagatca cagtttgtaa attcttcccg ccagagtgaa tgcggttacc tacactccag
     3961 attactgacc actggaggca gacaatatgg cgaacagcat cacggcggat gagattcggg
     4021 aacagttttc gcaggcaatg tcagccatgt accagcaaga agttccgcag tacggcacgc
     4081 tgctggaact ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct caactgcacg
     4141 aaaaaatggt aaatgcagac gagctggcgc gactgaatgt tgaacgtcat ggggcgattc
     4201 gcgttgggac tgcacaagag cttgctactc ttcggcggat gtttgccatt atggggatgt
     4261 acccggtgag ctattacgat ctctcgcagg cgggggtgcc ggtacattcg acagcatttc
     4321 ggcccattga tgatgcttct ctggcgcgta atcccttccg cgtttttacc tcgttactcc
     4381 gccttgagct tatcgagaac gaaattttgc gccagaaagc ggcggagatt ctacgtcagc
     4441 gcgatatctt caccccacgt tgtcgactac tgttagagga atatgagcag cggggcggtt
     4501 ttaacgaaac acaggcacag gagtttgtgc aggaagccct ggaaacgttt cgctggcacc
     4561 agtcagcaac ggtagatgaa gaaacctatc gcgccttgca caacgaacat cggttgattg
     4621 ctgatgtggt ctgttttcct ggatgccata tcaaccatct gacgccacgt acgctggata
     4681 ttgaccgggt gcagtcgatg atgcctgaat gcggaattga accaaaaatt ctgatcgaag
     4741 ggccgccgcg ccgcgaggta ccgattttac tacgccagac cagctttaaa gcactggaag
     4801 agacggtgtt gtttgcgggg cagaaacagg gcacgcatac tgcgcgcttt ggtgaaattg
     4861 agcagcgtgg cgtggcatta acgccgaaag ggcgacaact gtatgatgat cttctgcgta
     4921 acgctggaac cgggcaggat aatctcactc accaaatgca tttacaggaa accttccgca
     4981 cttttcctga cagtgagttt ttaatgcgtc agcaagggct ggcatggttc cggtaccgtc
     5041 tgacgccttc gggtgaggcg catcgtcagg cgattcatcc cggagacgat ccacagccct
     5101 taattgaacg tggttgggtc gcggcgcaac ccattaccta tgaagatttc ttgcccgtta
     5161 gcgcggcggg gatcttccag tcaaatctgg gtaatgaaac gcaggcacgc aatcacggta
     5221 atgccagtcg cgaagcattt gagcaggcgt tgggttgtcc ggttttggat gagttccagc
     5281 tttatcagga agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa attctgacca
     5341 tccgcctttg caaaaatttg cctgatttta caaacgaatc aggctcatcc catcgacata
     5401 aaaaaaatgc cgatttatgc atattctctc agttcaacaa ttggattatt aataaatatt
     5461 gtctagagtg agcggtcata aataagcact ttcttgccgc tgaaaacgac cagcgcggga
     5521 ccattcacaa caccagaagg actcactttc aggtatggat cgtagacgat ttattaaagg
     5581 ttcaatggct atggccgccg tgtgcggtac cagcggcatt gcttctcttt tttctcaggc
     5641 ggcattcgcg gcagattctg atattgccga cgggcaaacc cagcgttttg acttctccat
     5701 tctacagtca atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt
     5761 acctgacacg ctggcgacaa tgacgccgca ggcttataac agtattcaat acgacgccga
     5821 aaaatcgctc tggcataacg ttgagaaccg tcaactggac gctcagttct tccatatggg
     5881 aatgggattc cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg
     5941 tgaaattcac tttcgcccgg agttgttcaa atacaacgat gcaggtgttg ataccaaaca
     6001 attagaaggg caaagcgatc tcggctttgc cggttttcgc gtgtttaaag cccccgaact
     6061 ggcgcgccgt gatgtagtat catttctcgg cgcgagttat ttccgcgccg ttgatgatac
     6121 atatcaatac ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg acagtaaaga
     6181 agagttcccc gactttaccg ccttctggtt tgatacggta aaaccggggg caactacctt
     6241 taccgtttat gcgttgctcg atagcgccag cattactggt gcctataagt tcactatcca
     6301 ttgtgagaaa agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat
     6361 taaacagctg ggcattgcgc cgatgaccag tatgttcagc tgcggtacta atgaacgtcg
     6421 gatgtgcgat acaattcatc cgcaaattca tgactctgat cgtctgtcca tgtggcgggg
     6481 caacggcgag tggatttgcc gtccgctgaa taatccgcaa aaattgcagt tcaatgctta
     6541 caccgacaac aacccgaaag ggtttggttt attgcaactg gatcgtgact tctcccatta
     6601 tcaggacatt atgggctggt ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa
     6661 gtggggtaag ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa cgctggataa
     6721 cattgtctgc ttctggcagc cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca
     6781 gtatcgtctg tactggagtg cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc
     6841 gacgcgtacc ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac actatcccga
     6901 aaaatgggcg cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc
     6961 aaaaggcatt gagccggtga ttacgctgtc cagtggggaa gcgaagcaaa tcgaaattct
     7021 ctatattgaa cccattgatg gttatcgtat tcagtttgac tggtatccga cgtcggactc
     7081 cactgatccg gtcgatatgc ggatgtatct gcgttgtcag ggggacgcta tcagtgaaac
     7141 atggctgtat cagtatttcc cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt
     7201 gatgagttaa ttgttttttc ttcggcacct tcttcgggag gtgccgtctg gttaaacacg
     7261 atcccgctcg catttttccc taagttaaat gagtaatctg atggtgtgta tttcagatac
     7321 accttgtcag ccactaacag ggagtgcgta tgtttccaga ataccgagat ttaatatccc
     7381 gtctgaaaaa cgaaaatcct cgctttatgt ccttgttcga taaacacaat aaacttgatc
     7441 atgaaattgc cagaaaggaa ggttccgacg gtcgagggta caatgcggaa gtggtccgca
     7501 tgaaaaaaca aaagctacag ttaaaagatg agatgctcaa aatcctgcag caggagagcg
     7561 tcaaagaggt gtaaactttc ctaagccgcc agccaggcgg cttttttaac aacagcatgg
     7621 attgactgga gataagatga ctgaaacgat aaaagtaagc gaatcacttg aattacatgc
     7681 tgttgcggaa agtcacgtca cacctcttta tcagttaatc tgtaaaaata aaacctggtt
     7741 acagcagtcg ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt
     7801 gcagggtaat gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga
     7861 agatgaactt atcggcgtta tctcgtttaa tcgtattgaa ccactgaata aaactgctga
     7921 aatcggctac tggctggacg aatctcatca ggggcagggg atcatttctc aggcgctgca
     7981 ggcattgatt catcattacg ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg
     8041 ggtggacaat ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg
     8101 cctgaaacag gctgagttcc tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat
     8161 tatcggttca taataaccct gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga
     8221 cttttggacc acggacatga accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg
     8281 ttaactccac atggttttca attatcaccg cacctgaaat acggctctca ccttgtatta
     8341 caacgtgctc gtcgagcaga atcggtccac cacgtaccac ggcatttccg ccaatcaaga
     8401 catgatgttt taacacacaa ttaccttcca caatggcata ttccgccacc tgcgaactgt
     8461 aatgaatcgt cggaatggca tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga
     8521 ctttagcgca atcgcatagc cagacgttgt tctcttcatt accttcaata ctggcaaaat
     8581 caaaaacttc ggcgcgatgt tcaataaaag cataccggat gacggcatcg ccataaattt
     8641 gtgcctggtg gacaatacgc gaggcgctaa cccttgcgcg atcataaatt tgtagcagga
     8701 gttgatggtc aggcgttaag ccttgtgctg cgacgatcat agaatgttga ttaattaagg
     8761 cgtgaccaaa tattctacat tgcccgcaaa ccagtgagtc atgaatggtg acactgtcac
     8821 ttatatatgc accctggctg atttcactat tatcgatcca gacattatcc gttgcgtaaa
     8881 cctctcccca cagtacgctg gtgccggtga tgcgcgtatt accggaaatc accgttccgc
     8941 cgaacgcaat agcattctgg tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt
     9001 cgcgatcgat ccagccgcca gcggttcctg ctatcacatc gttaaaatcg ctaatggcga
     9061 taatctgccg taataacaca ttttttttag tgccatcttc ctgataacta aaggctcgct
     9121 gttcttcact tagacgatat ttgcgcatag cgttttccca caggtgactt actataaccg
     9181 tagcaaattc tgcggctctg gctatgctcg agaaaattcc ataaaatgca tttcaaatat
     9241 actttataaa ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc
     9301 ggcaggctac tttggtattg tgttggggac gatagggatg ggatttgcct ggcgctatgc
     9361 cagccaggtt tggcaggtca gccactggtt aggggatggg ctggtgattc tggcgatgat
     9421 catctgggga ttattgacta gcgcatttat tgcccgactc atacgctttc cgcatagcgt
     9481 gctggcggaa gttcgccatc cagtgctgag cagttttgtg agtttgtttc cggcaacgac
     9541 gatgctggtg gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt gcctgttcag
     9601 ctttggtgtc gtggttcagt tggcttatgc cgcctggcaa actgcgggat tatggcgcgg
     9661 atctcaccct gaagaagcta ccacgcctgg actgtatctg ccgacagttg ccaacaactt
     9721 tatcagcgca atggcctgtg gtgcgttggg ctacaccgac gccggtctgg tgtttttagg
     9781 cgcaggcgtt ttctcatggc taagccttga accggtgatc ttgcagcgtc tgcgtagttc
     9841 gggagaatta cccacggcac tgcggacatc actcggcatt cagctcgctc ctgcgctggt
     9901 ggcctgtagt gcctggctga gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct
     9961 tttcggttat ggactgctgc aactgctgtt tatgctacgt ctgatgccat ggtatctctc
    10021 ccagccattt aatgcttcat tctggagttt ctcgttcggc gtatctgcac tggcaaccac
    10081 cggtttgcat ctggggagtg gcagcgataa tggatttttc catacgctgg cggtgccgct
    10141 gtttatcttt accaatttta ttattgcaat actgctcatc cgtacttttg cgcttctgat
    10201 gcagggaaaa ttgttagtca gaaccgagcg cgccgtttta atgaaagcag aggacaaaga
    10261 atgatcattc gtgacgaaaa ctattttact gataaatatg aattaacccg cacacactct
    10321 gaagtaatgg aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct gggctgtggc
    10381 aatggtcgta acagtcttta cctggcagcc aatggttatg atgttgacgc atgggataaa
    10441 aatgccatga gtatcgccaa cgtcgagcgc attaaatcta ttgaaaatct ggataattta
    10501 cacactcggg tcgttgatct gaataacctc acatttgctg gacagtacga ttttattctt
    10561 tcgactgtgg tgctgatgtt ccttgaggct aaaaccatcc cagggctgat tgccaatatg
    10621 caacgttgca cgaaacctgg cggttacaac ctgattgtgg cggcgatgga tagcgctgat
    10681 tatccatgta ccgtcggctt cccgtttgcc ttcaaagaga gagaattacg tcgatattac
    10741 gaaggctggg agatggtgaa atacaatgaa gacgtcggcg agctgcaccg caccgacgcc
    10801 aacggtaatc gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa atgacccggt
    10861 aagaacaaag cgcgtgaaaa ttccccacgc tgagatagtt tactgttctt cttttcggta
    10921 agcatatttt ttatcgaagg gatg
//
LOCUS       DACAPB010000151        10882 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.151, whole genome shotgun sequence.
ACCESSION   DACAPB010000151 DACAPB010000000
VERSION     DACAPB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.151"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..200
                     /locus_tag="IT904_003532"
     CDS             <1..200
                     /locus_tag="IT904_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3763 domain-containing protein"
                     /protein_id="HAP3193266.1"
                     /translation="LPGSSAEGLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPGDW
                     LGRIEASLQDVSAQIRQAQQC"
     gene            194..1645
                     /gene="viaA"
                     /locus_tag="IT904_003533"
     CDS             194..1645
                     /gene="viaA"
                     /locus_tag="IT904_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAP3193267.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSAITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(1650..2642)
                     /gene="asnA"
                     /locus_tag="IT904_003534"
     CDS             complement(1650..2642)
                     /gene="asnA"
                     /locus_tag="IT904_003534"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAP3193268.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            2794..3252
                     /gene="asnC"
                     /locus_tag="IT904_003535"
     CDS             2794..3252
                     /gene="asnC"
                     /locus_tag="IT904_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAP3193269.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            3342..3785
                     /gene="mioC"
                     /locus_tag="IT904_003536"
     CDS             3342..3785
                     /gene="mioC"
                     /locus_tag="IT904_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAP3193270.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            4164..6053
                     /gene="mnmG"
                     /locus_tag="IT904_003537"
     CDS             4164..6053
                     /gene="mnmG"
                     /locus_tag="IT904_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP3193271.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            6117..6740
                     /gene="rsmG"
                     /locus_tag="IT904_003538"
     CDS             6117..6740
                     /gene="rsmG"
                     /locus_tag="IT904_003538"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP3193272.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            7357..7737
                     /gene="atpI"
                     /locus_tag="IT904_003539"
     CDS             7357..7737
                     /gene="atpI"
                     /locus_tag="IT904_003539"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAP3193273.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            7746..8561
                     /gene="atpB"
                     /locus_tag="IT904_003540"
     CDS             7746..8561
                     /gene="atpB"
                     /locus_tag="IT904_003540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP3193274.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            8608..8847
                     /gene="atpE"
                     /locus_tag="IT904_003541"
     CDS             8608..8847
                     /gene="atpE"
                     /locus_tag="IT904_003541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAP3193275.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            8909..9379
                     /gene="atpF"
                     /locus_tag="IT904_003542"
     CDS             8909..9379
                     /gene="atpF"
                     /locus_tag="IT904_003542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP3193276.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            9394..9927
                     /gene="atpH"
                     /locus_tag="IT904_003543"
     CDS             9394..9927
                     /gene="atpH"
                     /locus_tag="IT904_003543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP3193277.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSSKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            9940..>10882
                     /gene="atpA"
                     /locus_tag="IT904_003544"
     CDS             9940..>10882
                     /gene="atpA"
                     /locus_tag="IT904_003544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP3193278.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTK"
BASE COUNT         2682 a         2724 c         2817 g         2659 t
ORIGIN      
        1 cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa ctggaagagc
       61 ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa tgtctgttta
      121 tccctggcga ctggttaggt cgcattgaag ccagcctaca ggatgtcagt gcacagattc
      181 gccaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg tcagcgaaga
      241 gggattgatc gaagagatga tcatcgcgct gctggcctca ccgcagctgg cagtcttctt
      301 tgaaaaattc ccacgcctga aggcagcaat cactgatgat gttccccgct ggcgtgaggc
      361 gctgcgcagt cggctgaaag atgcccgagt cccgccagaa ctcaccgaag aggtgatgtg
      421 ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac cacagatcct
      481 ggacttactg catcgtctga attccccatg ggcagaacaa gcccgacagt tggttgatgc
      541 taacagcgcg atcacttcag cgttacacac actttttctc cagcgttggc gtttaagtct
      601 gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg aacaactgtt
      661 aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc tcgcagataa
      721 caataccgca gctggtcgtc tgtgggatat gagcgccggc cagcttaaac gtggcgacta
      781 tcagttgatt gtgaaatacg gtgaatttct taacgaacag ccggaactga aacgcctggc
      841 agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg cgcagatgga
      901 aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg atggtctgca
      961 acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag ggataacgga
     1021 actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct atcgcctgca
     1081 cggtgagtcg tggcgtgaaa aagtgatcga acgtccggtg gtacataaag attacgatga
     1141 acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg gcggctttaa
     1201 tgaacagtgt gcgaaagcgt tctgcctggc cttgatgcgc attgctctcg cagaaaaccg
     1261 gcgctgctat attatgctat tttccaccga gatcgtccgt tatgagcttt caggcccaca
     1321 aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca ccgatcttgc
     1381 cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg atgccgatgc
     1441 ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga gtaaagtgaa
     1501 agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg cacacggcaa
     1561 acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga tgcgaagccg
     1621 cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct ctcgcgaaca
     1681 gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag cagcagcata
     1741 gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc gcgcagcagc
     1801 gcctgatgcc actccagctc caggcgatct tcgtcaccgg tcagcgccag ttgatgcttc
     1861 agcgtgtcgg catctacacg gatccccatg gaggaaagct caaacgcatc ttccagtacc
     1921 gggttccaca ccagaatatc gccgttcaga cccgcatggc ccagctctga cggggtgctc
     1981 cagtcatcat aatccggtgc gcgcacgtcg tggcgatgac catcgctcag cttgccgcca
     2041 atcccgacaa ggaataccgc gccaagatct ttcgctatcg cccgctcacg ccctttggca
     2101 tcaagatccg gataacgaga cagtaactcc tggctgtgta cgaagtggat ctgatccggc
     2161 aggaacggtg ccaggccaaa ctcttcgcta accgcagctt cggttgcttt aattcccgcc
     2221 cagatcgcct ctaccgtgct tttcagagtc gagaattgac gctcaccgtc gcccattacg
     2281 cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg gtcttcatcg
     2341 gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc gtgttgccct
     2401 aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc atcaggcaga
     2461 gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg cgtgccatcc
     2521 cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc ttccagttga
     2581 cgagaaaagt gagatttcac gaagctaatt tgacgttgtt tggcaatgta agcggttttc
     2641 attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg acacacatgc
     2701 aataatcatt caataaaaag cctaactcac ttttgattca ttaaaaaaga aggctaaaat
     2761 agaatgaatc atcaatccga ataagaaaat cctatggaaa attatctgat cgacaatctg
     2821 gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc cgaactggcg
     2881 aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat gaagcaggcg
     2941 gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta tgacgtaggc
     3001 tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct ggcaaagctg
     3061 gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag catctttata
     3121 aaagtgatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa gatccaaaca
     3181 attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat catgcgtacc
     3241 atcaagccat aatcggcttt tttaatccca tacttttcca caggtagatc ccaacgcgtt
     3301 cacggcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat atcactctta
     3361 tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct gaaaagctgg
     3421 aagaggcggg ttttaccacc gaaacgctgc acggtccgct gttggaagat ttatctgcct
     3481 cggggatctg gctgattatc agctccaccc acggtgccgg agatattccg gacaaccttt
     3541 ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc cgctttggcg
     3601 caatcggtat tggtagtcgt gaatatgaca ccttttgtgg ggctatcgat aaactcgagg
     3661 cagaactcaa agattccggt gcaaaacaga caggcgaaac actgaagatc aacattcttg
     3721 atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt aatttactca
     3781 aataagtata cagatcgtgc gatctactgt gaataactct gtcaggaagc ttggattaac
     3841 cggtagttat ccaaggaaca acggttgttc agtttttgag ttgtgtataa cccctcattc
     3901 tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat cctttccagg
     3961 ttgttgatct taaaagccgg atccttgtta tccacagggc agtgcgatcc taataagaga
     4021 tcacaataga acagatctct aaataaatag atcttctttt taatacccag gatcccaggt
     4081 ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc cattttcata
     4141 ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc atcatcattg
     4201 gcgggggtca tgcaggcacc gaggccgcga tggccgcggc gcgtatgggt caacagactc
     4261 tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg gcgatcggcg
     4321 gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg atggcgaaag
     4381 cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga ccggcggttc
     4441 gcgctacccg agctcaggcg gatcgtgtgc tctaccgtca ggcggtacgt acggcgctgg
     4501 agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt gtcgaaaacg
     4561 atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa gccgtcgtgc
     4621 tcaccgttgg gacattcctc gacggtaaaa ttcatatcgg tctggataat tacagcggtg
     4681 gccgtgctgg tgatccgccg tccattccgc tttctcgccg tttgcgtgaa ctgccgctgc
     4741 gcgttggtcg tctgaaaacc gggacaccac cgcgtattga tgctcgaact atcgacttta
     4801 gtgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt atgggcaatg
     4861 cgtcccagca tccccagcag gtgccgtgtt atatcactca taccaacgag aaaacccatg
     4921 atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc gaaggtgtcg
     4981 gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgccgac agaaatcagc
     5041 atcagatctt ccttgaaccg gaaggactga cctctaacga aatttatccg aacggtatct
     5101 ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag gggatggaaa
     5161 acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat cctcgcgacc
     5221 tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt cagattaacg
     5281 gcactaccgg ttacgaagaa gccgctgcgc aaggtttact ggccggtctt aacgctgccc
     5341 gtctgtctgc tgacaaagaa ggttgggctc cggcgcgttc tcaggcgtat ctcggcgtac
     5401 tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt acctcgcgcg
     5461 cagaatatcg tctgatgctg cgcgaagata acgcggatct gcgtttgact gaaatcggtc
     5521 gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa cttgagaata
     5581 tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg gaagctgcag
     5641 ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt gaagatctgc
     5701 tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt gcccctgcgt
     5761 tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa ggttatatcg
     5821 cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg ctacccgcga
     5881 cactggatta ccgccaggta tccggtcttt ctaacgaagt gatcgccaaa cttaacgatc
     5941 acaaaccggc ctctatcggc caggcttcac gtatttctgg cgtcacgcct gcggccatct
     6001 ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca taacacatta
     6061 aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta atcaccgtgc
     6121 tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttaccgat caccagaaaa
     6181 accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac aacctgactt
     6241 cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt gtggtggcac
     6301 cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg ccaggcattc
     6361 cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt ggtaaacgcg
     6421 tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa ccagtacaga
     6481 gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc cgcgcttttg
     6541 cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa ggccgtttct
     6601 acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa gaatatcagg
     6661 tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat ctggtggtga
     6721 ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac cggttagact
     6781 gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc acgttcactc
     6841 ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt cattagtgaa
     6901 aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa acattattaa
     6961 aaatgtcaat gggtggtttt tgttgtgtaa atgtcattta ttaaaacagt atctgttttt
     7021 agactgaaat atcataaact tgcaaaggca tcatttgcca agtaaataaa tatgctgtgc
     7081 gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta cgcgttattt
     7141 acagtttttc atgatcgaac agggttagca gaaaagtcgc aattgtatgc actggaaaaa
     7201 tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg ggcgcaccgt
     7261 ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt atacgacacg
     7321 cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct cgtgagtcga
     7381 aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag tggattgctg
     7441 ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct ggcagtcttt
     7501 ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac accagcgaaa
     7561 ggccgggtgg cctggacatt cgcatttggc gaagctttca aagttctggc gatgttggtg
     7621 ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat cgttacgtgg
     7681 gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa agggtaaaag
     7741 gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac ctgaataacc
     7801 ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca gccaccttct
     7861 ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg ttcctggttt
     7921 tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt cagaccgcga
     7981 ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat ggcaaaagca
     8041 agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg aacctgatgg
     8101 atttactgcc tatcgacctg ctgccgtaca ttgctgaaca tgtactgggt ctgcctgcac
     8161 tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg ggcgtattta
     8221 tcctgattct gttctacagc atcaaaatga aaggcatcgg cggcttcacg aaagagttga
     8281 cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt gaaggggtaa
     8341 gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg tatgccggtg
     8401 agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg atcctgaatg
     8461 tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc ttcatggttc
     8521 tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac actactacgt
     8581 tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct gctgtacatg
     8641 gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat cggcatcctc
     8701 gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct gctgcgtact
     8761 cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt aggtctgggt
     8821 ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga gcaatatcag
     8881 aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc tcggccaggc
     8941 catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc cattaatggc
     9001 agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag aacgagcaca
     9061 taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag cgaaagcgga
     9121 agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg acgaagcgaa
     9181 agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcgg aaattgaagc
     9241 cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg ctgttgctgg
     9301 cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca tcgtggataa
     9361 acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac ggtagctcgc
     9421 ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga acgctggcag
     9481 gacatgctgg cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga gcttctctct
     9541 ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg tgagcaactg
     9601 gacgaaaacg gtcagaacct gattcgggtt atggctgaaa atggtcgtct taacgcgctc
     9661 ccggatgttc tggagcagtt tattcacctg cgtgccgtga gtgaggctac cgctgaggta
     9721 gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc tgctgcgatg
     9781 gaaaaacgtc tgtcaagcaa agttaagctg aattgcaaaa tcgataagtc tgtaatggca
     9841 ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg tcgtcttgag
     9901 cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa ttccaccgaa
     9961 atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga agctcacaac
    10021 gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct ggccgattgt
    10081 atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact gaacctcgag
    10141 cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga aggcatgaaa
    10201 gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct gggccgtgtg
    10261 gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga cggcttctct
    10321 gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca gccggtacag
    10381 accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg tgaattgatc
    10441 atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat caaccagcgc
    10501 gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac catttctaac
    10561 gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt ggtagcaacc
    10621 gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc aatgggcgaa
    10681 tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc taaacaggct
    10741 gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga agcattcccg
    10801 ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt taacgccgaa
    10861 tacgttgaag ccttcaccaa ag
//
LOCUS       DACAPB010000152        10848 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.152, whole genome shotgun sequence.
ACCESSION   DACAPB010000152 DACAPB010000000
VERSION     DACAPB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.152"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..208)
                     /locus_tag="IT904_003545"
     CDS             complement(<1..208)
                     /locus_tag="IT904_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193279.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGP"
     gene            complement(451..657)
                     /locus_tag="IT904_003546"
     CDS             complement(451..657)
                     /locus_tag="IT904_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAP3193280.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            970..1059
                     /gene="kdpF"
                     /locus_tag="IT904_003547"
     CDS             970..1059
                     /gene="kdpF"
                     /locus_tag="IT904_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAP3193281.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1059..2732
                     /gene="kdpA"
                     /locus_tag="IT904_003548"
     CDS             1059..2732
                     /gene="kdpA"
                     /locus_tag="IT904_003548"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAP3193282.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IARLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQAASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2755..4803
                     /gene="kdpB"
                     /locus_tag="IT904_003549"
     CDS             2755..4803
                     /gene="kdpB"
                     /locus_tag="IT904_003549"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAP3193283.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4812..5384
                     /gene="kdpC"
                     /locus_tag="IT904_003550"
     CDS             4812..5384
                     /gene="kdpC"
                     /locus_tag="IT904_003550"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAP3193284.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5377..8061
                     /gene="kdbD"
                     /locus_tag="IT904_003551"
     CDS             5377..8061
                     /gene="kdbD"
                     /locus_tag="IT904_003551"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAP3193285.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            8058..8735
                     /gene="kdpE"
                     /locus_tag="IT904_003552"
     CDS             8058..8735
                     /gene="kdpE"
                     /locus_tag="IT904_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAP3193286.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            9010..9114
                     /locus_tag="IT904_003553"
     CDS             9010..9114
                     /locus_tag="IT904_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAP3193287.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9425..>10848
                     /gene="speC"
                     /locus_tag="IT904_003554"
     CDS             9425..>10848
                     /gene="speC"
                     /locus_tag="IT904_003554"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAP3193288.1"
                     /translation="MSELKIAVSRSCPDCFSTHRTCVNIDESNYIDVAAILLSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERIPAEYLSRISGVFEPSEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPG"
BASE COUNT         2398 a         2748 c         3008 g         2694 t
ORIGIN      
        1 gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcagcggat
       61 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
      121 caccgatgcg taccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc
      181 cctgacgcgc cgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc
      241 tgctgacagg cgaaacccta cagatttata agaaagcctt aagtgagata tctgtcagat
      301 aagaacaatt aggaaaatat ttatatatat ttcatgaata tatgtttcga ttggtataac
      361 gatgtctaaa tattcctctg aacatcttcc tgatacaaaa aaccgccagc gtttccgctg
      421 gcggttttca atgtattcaa aactcagcaa tcaataaaaa tcaccagttg ccttttcggc
      481 ctgatccatc cacaccggtt tatcgctggt tttcgaccag acgcgatgca ggtagctgta
      541 aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc gccagaacgc ccgctgcaac
      601 cgcataagta cggcgtaaaa agataagcca tgcaggatat tctctgtaga gttccatact
      661 tttctccccc ttttgtatct acccggtgaa tggcaccgga aaaatgaatt tgtttatctg
      721 atgaaaatag taccgccttt tgtgtaattt tactactcat ccgaccactt atttttgctt
      781 attgatggtt tatttacatt catcctgtaa ttaagttaca caaaagttaa attaatacta
      841 aacattagtt aaatcatggc ttttgccatt tttatacttt ttttacaccc cgcccgcaga
      901 tttttgcgaa atctttgcag ccagaattct acccttccgg tatcactttt aggccactgg
      961 aggtgcactg tgagtgcagg cgtgataacc ggcgtattgc tggtgttttt attactgggt
     1021 tatctggttt atgccctgat caatgcggag gcgttctgat ggctgcgcaa gggttcttac
     1081 tgatcgccac gtttttactg gtgttaatgg tgctggcgcg tcctttaggc agcgggctgg
     1141 cgcggctgat taatgacatt cctcttcccg gtacaacggg cgttgagcgc gtactttttc
     1201 gcgcacttgg cgtctctgac cgtgagatga actggaagca atatctttgt gccattctcg
     1261 gcctgaacat gctggggctg gcggtgctgt tttttatgtt gctcggtcag cactatctgc
     1321 cgcttaatcc acagcagttg ccagggctgt cgtgggatct ggcgctgaat accgccgtca
     1381 gctttgtcac caataccaac tggcaatctt atagcggtga aaccacgttg agctatttca
     1441 gccagatggc gggcttaacg gtgcaaaact ttctttctgc cgccagcggg attgcggtga
     1501 tttttgccct catccgtgcg tttacccgcc agagcatgag cacgctcggg aatgcctggg
     1561 tcgatctgct acgcatcacg ttatgggtgc tagtccctgt ggcgttgttg attgcactgt
     1621 tttttattca acaaggtgcg ctgcaaaact ttctgcctta tcaggctgtg aataccgttg
     1681 aaggagcgca acagctgtta cccatggggc ctgtagcttc tcaggaagcg atcaagatgc
     1741 tcggtactaa cggcggtggc ttctttaatg ccaactcgtc gcatccgttt gaaaacccaa
     1801 ccgcactgac caacttcgtg cagatgctgg cgatcttctt gatcccaacg gcgctgtgct
     1861 ttgcctttgg tgaagtgacg ggcgatcgcc gccaggggcg catgttgctg tgggcgatgt
     1921 cagtgatttt tgtcatctgc gtaggcgtgg tgatgtgggc agaagttcag ggtaatcctc
     1981 atctgctggc actgggcacg gacagcagca tcaatatgga aggtaaagag agccgtttcg
     2041 gcgtgctggt cagtagcctg tttgcggtcg tgacgacggc ggcttcctgt ggcgcggtga
     2101 ttgcgatgca tgattcgttt accgctctcg gtggcatggt gccgatgtgg ctgatgcaaa
     2161 ttggtgaagt ggtgttcggc ggtgtcggtt ctggtcttta cggcatgatg ctgtttgtcc
     2221 tgctggcggt gtttattgcc aggctgatga ttggtcgtac accggaatat ctgggtaaaa
     2281 aaatcgacgt acgcgagatg aaactgactg cactggcaat tctggttacc ccgacgctgg
     2341 tgctgatggg cgcggcgttg gcgatgatga ccgacgccgg acgtagcgcc atgctcaacc
     2401 ctggcccgca tggttttagc gaagtgctgt acgccgtgtc atccgccgct aacaacaacg
     2461 gcagcgcctt tgccggatta agcgccaact ctccgttctg gaactgttta ctggcgttct
     2521 gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat ggcaattgcc ggttcgctgg
     2581 tgagtaaaaa gagccaagcc gccagctccg gcacgctgcc aacgcacggc ccgctgtttg
     2641 ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact gacctttatc cctgccctgg
     2701 cgcttggtcc ggtggcggaa tatctctcct gatgatattg agtgagcact gaatatgagt
     2761 cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc aggcgctgaa agaagcggtg
     2821 aaaaaattaa acccgcaggc gcaatggcgc aatccggtga tgtttatcgt ctggatcggc
     2881 agtctgctga ccacctgtat tagcatcgcg atggcaagcg gtgcgatgcc cggcaatgcg
     2941 ctgtttagcg cggccattag cggttggctg tggatcaccg tactgttcgc taatttcgcc
     3001 gaggcgctgg cagaaggccg cagtaaagcg caggccaaca gtctgaaagg ggtgaaaaaa
     3061 accgcttttg ctcgcaagct gcgcgaaccg aaatatggcg ctgtggcaga caaagttcct
     3121 gccgatcaac tgcgtaaagg tgacatcgtg ctggtagaag ctggcgatat tatcccctgc
     3181 gatggtgaag tcattgaagg cggcgcatcg gtcgatgaaa gcgccatcac cggggaatcg
     3241 gcaccggtga tccgtgaatc cggcggcgat tttgcctccg tcaccggcgg cacgcgtatt
     3301 ctttctgact ggctggtgat tgagtgtagc gttaaccccg gcgagacatt tctggatcgg
     3361 atgatcgcga tggtggaagg cgcacagcga cgtaaaacgc cgaacgagat tgccctgacc
     3421 atcctgctga ttgccctgac tatcgtcttt ttactggcaa ccgccacgct gtggccgttt
     3481 tccgcgtggg gcggtaatgc agtcagcgta acggtactgg tggcgctgct ggtctgtctg
     3541 atcccaacca ctattggcgg cctgttgtca gcgatcggcg tcgccgggat gagccggatg
     3601 ctgggcgcga atgtgattgc caccagcgga cgtgcagttg aagcggcagg tgacgttgac
     3661 gttctgctac tggataaaac cggcaccatc acactcggta accgtcaggc gtcggagttt
     3721 atccccgcgc agggcgtgga tgaaaaaacg ctggctgacg ccgcacaact ggcttcgctg
     3781 gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg ccaagcagcg ttttaacctg
     3841 cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac cgtttactgc gcaaagccgg
     3901 atgagcggga tcaacatcga caaccgcatg atccgtaaag gttctgtcga tgccattcgc
     3961 cgccatgttg aggctaacgg cggtcacttc cctgccgatg ttgatcaaaa agtcgatcag
     4021 gttgcgcgtc agggagccac gccgctggtg gtggtggaag gttctcgtgt gctgggcgtt
     4081 attgcgctga aagatatcgt caaaggcggt attaaagagc gcttcgccca gctgcgcaaa
     4141 atgggcatta aaacggtgat gattaccggc gataaccgtc tgactgccgc cgccattgct
     4201 gcggaagcgg gcgtcgatga ttttctcgcc gaagcgacac cggaggccaa gctggcattg
     4261 attcgtcagt atcaggcgga aggccgtttg gtggcgatga ccggcgacgg caccaatgat
     4321 gctccggcgc tggcgcaggc agatgtcgcg gtagcgatga actccggtac tcaggcggcg
     4381 aaagaagcgg gcaatatggt ggatctcgac tctaacccga ccaagttgat tgaggtggtg
     4441 catattggca aacagatgct gatgacccgt ggctcgctga ccaccttcag tattgccaac
     4501 gatgtggcga aatacttcgc cattattccg gcggcgttcg cggcaacgta tccgcagtta
     4561 aatgcgctga acatcatgcg cctgcattcg cccgactccg ccattctcag tgcggtcatt
     4621 ttcaacgcct tgattatcgt ctttttgatt cccctggcgt taaaaggcgt gagttataaa
     4681 ccgcttaccg cttctgccat gttgcgccgt aacttatgga tttacggtct gggtgggctg
     4741 ctggtgccgt ttattggtat caaagtcatt gatttactgc tgaccgtttg cggtctggtg
     4801 tgaggtttac catgagtgga ttacgtccgg cattatcgac atttatcttt ctgttattga
     4861 ttactggcgg tgtttacccg ctgctgacca ccgcgctggg gcaatggtgg tttccctggc
     4921 aggccaacgg ttcgttgatc cgtgaaggtg atacggtgcg tggctcggca ttaatcgggc
     4981 agaattttac cggcaacggc tattttcatg gtcgcccgtc ggcaacggca gaaatgccct
     5041 ataatccaca ggcttctggc gggagcaatc tggcagtcag taaccctgag ctggataaac
     5101 taatagccgc acgcgttgct gcattacggg ccgctaaccc ggatgccagc acgagcgttc
     5161 cggttgaact ggtgacggca tcggcaagcg ggctggacaa taatatcacc ccgcaagcgg
     5221 cggcctggca aatcccacgc gtggcgaaag cgcgtaatct cagcgttgaa cagctcacgc
     5281 aactgatcgc aaaatacagc caacaaccgc tggtgaaata tatcggccag ccggtcgtca
     5341 acattgttga actcaatctg gcgctggata aacttgatga ataacgaacc cttacgtccc
     5401 gaccccgatc gtctgctgga acaaactgcc gcgccgcatc gggggaagct gaaagttttc
     5461 ttcggtgcct gtgcaggcgt cgggaagacc tgggcgatgc tggcagaagc acagcgactg
     5521 cggacgcaag ggctggatat tgtggttggc gtggtagaaa cccacgggcg gaaagatacc
     5581 gccgccatgc tggaagagct ggctgttctg ccgccaaaac gcctggcgta ccgtgggcgg
     5641 catatcagcg agtttgatct cgatgccgcc ctcgcccgcc gtccggcgct gatcttaatg
     5701 gacgaactgg cgcacagtaa tgcgcctggt tctcgtcatc ccaaacgctg gcaggatatt
     5761 gaagagctgc tggaagcagg cattgatgtt tttactaccg tcaacgttca gcatctggaa
     5821 agtctgaatg atgtggtcag cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat
     5881 ccttttttcg atgccgccga cgacgtggtg ctggtggact tgcccccgga cgatctgcgc
     5941 cagcggctga aagaaggcaa agtctatatt gccgggcagg cggagcgcgc cattgaacat
     6001 tttttccgca aaggcaatct gatcgccctg cgcgaactgg cactgcgccg tactgccgat
     6061 cgcgttgatg agcaaatgcg cgcctggcgg gggcatcctg gcgaagagaa agtctggcac
     6121 acgcgcgacg cgatcctttt atgcatcggc cataacaccg gcagcgaaaa actggtccgc
     6181 gcggcggcgc ggctggcgtc acggctgggg agcgtctggc acgcggtgta tgttgaaacc
     6241 cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg
     6301 gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc cagcggaaga gaaagcggta
     6361 gtgcgttatg cccgtgaaca taatctcggc aagattattc tcggtcgccc agcctcgcgc
     6421 cgctggtggc gtcgggaaac gtttgctgac cgactggcgc gcatcgcccc cgatctcgat
     6481 caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc gccggatagc
     6541 cgctctttta aagacaagtg gcgggtacag attcagggat gcgtggttgc cgccgcgtta
     6601 tgcgccgtta tcaccttaat tgccatgcag tggctgatgg cgtttgatgc cgccaacctg
     6661 gtgatgctgt atctgcttgg cgtggtggtg gtggcgctat tttatggacg ctggccttcg
     6721 gtggttgcta ccgtcattaa tgtggtgagt ttcgatctct tttttatcgc cccacgcggc
     6781 acgctcgccg tctctgatgt gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg
     6841 ctggtgatcg ggaaccttac tgcgggtgtg cgttatcagg cgcgggtagc ccgttaccgc
     6901 gagcaacgca cacggcactt atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg
     6961 caggacatcg ccgccaccag cgaacaattt attgcctcca cgtttcatgc ccgcagtcag
     7021 gtgttgttgc ccgatgacaa cggtaaattg cagccgttaa cacatccgca aggaatgacg
     7081 ccgtgggacg atgccatcgc gcagtggagt tttgataaag gcctgcctgc gggtgcgggc
     7141 accgacacgt tacccggtgt accgtaccaa attttgccgt taaaaagcgg cgagaaaacc
     7201 tacggactgg tggtggtgga accggggaat ctgcgtcagt tgatgattcc ggagcagcag
     7261 cgcctgctgg agacgtttac gctgttagtc gccaatgcgc ttgagcggct gacgctaacc
     7321 gccagcgaag aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa cgccctgctg
     7381 gcggcgcttt cgcatgattt acgcactccg ctcacggtgt tgttcggtca ggcagaaatc
     7441 ttaacgctcg atctggcaag cgaaggatcg ccccacgccc gccaggccag cgagatccgt
     7501 cagcacgtgc tgaacactac ccgactggtg aataatctac tggatatggc gcgaattcag
     7561 tccggcggct ttaatttgaa gaaagagtgg ttaacgctgg aagaagtggt cggcagcgcg
     7621 ctgcaaatgc tggaaccggg tttatcgtcg cccatcaatc tttctctgcc agaaccgctg
     7681 accttaatcc acgttgacgg gccactcttt gaacgggtgc tgattaatct gctggagaac
     7741 gcggtgaaat atgcgggtgc gcaggccgaa attggtatcg atgcccacgt tgagggcgaa
     7801 aatctacaac tggatgtctg ggataacggc cccggtcttc cgccaggcca ggagcagacg
     7861 atatttgata agtttgctcg cgggaataaa gagtcggcag taccgggggt agggcttgga
     7921 ctggcaattt gtcgggcgat agtggatgta cacgggggca ctattaccgc gttcaaccga
     7981 ccggaaggtg gtgcctgttt tcgtgttaca cttccccagc aaactgcccc tgaacttgaa
     8041 gaatttcatg aggatatgtg acaaacgttc tgattgttga agatgaacag gctattcgtc
     8101 gctttctgcg cacggcgctg gagggcgacg ggatgcgcgt ctatgaggcc gaaacgctgc
     8161 aacgcggctt gctggaagcg gctacccgta agccagattt gattattctc gatctcggcc
     8221 tgcccgatgg tgatgggatt gagtttatcc gcgacctgcg ccagtggagt gcggtgccgg
     8281 tgattgtgct ttccgcacgc agcgaagaga gcgacaaaat cgccgcgctg gatgccggag
     8341 cggatgatta tctgagtaag ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg
     8401 cattacgccg ccactctgcc accaccgcac ccgatccgct ggtgaaattt tccgatgtta
     8461 ccgtcgattt agccgcccgc gtgattcacc ggggtgagga agaggtgcat ctcacaccaa
     8521 ttgagttccg cctgctggcg gtgctgctca acaatgccgg aaaagtactc acccagcgcc
     8581 agctccttaa ccaggtgtgg gggccaaacg cggtcgaaca cagtcactat ttgcgtattt
     8641 atatgggaca tctgcgacaa aaactggaac aggatcccgc ccgcccacgc catttcatta
     8701 ctgaaaccgg tattgggtat cggtttatgc tttgaattgt tattcaataa attcgcttaa
     8761 atagtgatta atttattatt aaacctgcac caaatattat tatttggcag gtttaatttc
     8821 ttaacgcaaa tataaaacac aaaattacaa catttaaata acaaaacagc gtctaatatg
     8881 gcgctattca taattaatga tttattatct ggggtatgat cgttttttgt tgatcttctt
     8941 cacagattat agccatttca tggatagaat aactctacct tcaactgaca cagcaagagg
     9001 taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc
     9061 atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct
     9121 gactaaaggg caccccagcg tacaggtctc cctgacttta agcattacag gttaatacct
     9181 gtattcctcg gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag
     9241 catatgcaac ttgaaggatg acgagtatag ccagttaccg ggctggtctg ggttattgca
     9301 tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc
     9361 gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
     9421 caaaatgtca gaattaaaaa ttgccgttag tcgttcttgc ccggattgtt tttccactca
     9481 tcgtacatgc gtgaatattg acgaaagtaa ttatattgac gttgccgcca ttcttttatc
     9541 ggtcaatgat gtggaacgtg gaaaactcga tgaaatagac gctactggct atggcattcc
     9601 tgtttttatt gcaacggaaa atgaagaacg tataccagca gaatatcttt cacgtatttc
     9661 tggtgtcttc gaacctagcg aagcacgtaa agaattttat ggtcgccagt tagaaaccgc
     9721 tgccagccat tatgaaactc aactgcgccc acctttcttc cgcgcactgg tcgattatgt
     9781 caatcaaggt aacagcgcgt ttgattgccc tggtcatcag ggcggcgaat ttttccgtcg
     9841 ccatccggcg gggaatcagt ttgtggaata ctttggtgag gcgctgttcc gtgccgactt
     9901 gtgcaacgcc gacgtagcga tgggcgatct gctgattcac gaaggcgcgc catgcattgc
     9961 acagcaacat gcggcaaaag tgtttaatgc cgataaaacc tacttcgttt taaatggcac
    10021 ttcatcttct aacaaagtgg ttttaaacgc cctgctaaca ccgggtgatc tggtgctgtt
    10081 tgaccgcaat aaccacaaat ctaaccacca cggagcgttg ctacaggctg gtgcaacacc
    10141 ggtttatctg gaaacggcac gtaacccgta tggctttatc ggtggcattg atgcgcactg
    10201 ttttgaagaa agttacctgc gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga
    10261 ggctcgtcct ttccgcctcg ctgtgattca gttaggcacc tacgacggta cgatttataa
    10321 cgcccgccag gtggtggata aaattggtca tctgtgtgac tacatcctgt ttgactcagc
    10381 atgggtcggc tatgaacagt ttattccgat gatggcggac tgttcgccgc tgttgctgga
    10441 tcttaatgag aacgatccgg gtattctggt tacgcaatct gtgcataaac aacaggctgg
    10501 tttttctcag acttcacaaa ttcataaaaa agacagccac atcaaagggc aacagcgtta
    10561 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
    10621 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
    10681 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg
    10741 tcagcatatt cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
    10801 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccagg
//
LOCUS       DACAPB010000153        10708 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.153, whole genome shotgun sequence.
ACCESSION   DACAPB010000153 DACAPB010000000
VERSION     DACAPB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.153"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..125
                     /locus_tag="IT904_003555"
     CDS             <1..125
                     /locus_tag="IT904_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP3193289.1"
                     /translation="PDLIWSLPDGEIRRYDSRLNIICRECRKSEVMQRILAFYQ"
     gene            328..753
                     /locus_tag="IT904_003556"
                     /pseudo
     CDS             328..753
                     /locus_tag="IT904_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            844..1893
                     /locus_tag="IT904_003557"
     CDS             844..1893
                     /locus_tag="IT904_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAP3193290.1"
                     /translation="MRVLLQPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEVVLRRSLGLPAEKNCSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPSQEKTVVSITVDPESPESFMKLPKRHRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            1911..2288
                     /locus_tag="IT904_003558"
     CDS             1911..2288
                     /locus_tag="IT904_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP3193291.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVRSRPQ
                     CCDDDAMIICECMARLKKNNSDLHDLLVDYYVGGMTFMALARKHGRSDCWVGRMLQKA
                     EGVVEGMLMVLDLRLEMDADCSK"
     gene            complement(2444..2968)
                     /locus_tag="IT904_003559"
     CDS             complement(2444..2968)
                     /locus_tag="IT904_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAP3193292.1"
                     /translation="MKLWPVLTGIALSFTLIACKAPTPPKGVQPITNFDANRYLGKWY
                     EIARLENWFERGLEQVSATYEKRNDGGIRVLNRGYDPTKNKWSESEGKAYFTGDTKTA
                     ALKVSFFGPFYGGYNVIKLDDEYKYALVSGPNREYLWILARTQTIPDNVKADYVRTAQ
                     KLGFNVNELLWVKQ"
     gene            3161..4120
                     /locus_tag="IT904_003560"
     CDS             3161..4120
                     /locus_tag="IT904_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HAP3193293.1"
                     /translation="MIKKPVIGISGCLGGSAVRFDGGHKRDDFLMDKLVEWVTFRPVC
                     PEMAIGLPVPRPALRLVRSKQGNIRMCFSHDQNEDVTERMTEFSRSYMDKLKDVSGFV
                     VCAKSPSCGMERVRVYDENGNRGRKDGVGLFTSTLMEKFSWLPVEEDGRLHDPVLREN
                     FIERVFALHELNHLYKEKLSRRELLAFHSRYKLQLLAHSQAGYKDMGPFVAAIHEWAD
                     LESYFEVYRDNLMAILRKPASRKNHTNVLMHIQGYFSNYLSTRQRKELSEVILNYRFG
                     TLPLLAPLTLLKHYLGEYPNDYLLTQNYFDPYPEELALRLMVN"
     gene            4527..5240
                     /locus_tag="IT904_003561"
     CDS             4527..5240
                     /locus_tag="IT904_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3193294.1"
                     /translation="MPYICSIILVLNSFGVRIGKEDILFKKGNAVLIEYNLKDLFSSN
                     TDHLMIVDIEEKTVNDFFKNYTLSPFSVRRFYPSYLIVECEDFSLLRNLVACLNCDSK
                     TVDVVRNQISLACLAVLSSEKIVQSFLFGSLNSLGCKVKAIIHSDISASWRLCDISSR
                     LYMSESLLKRKLKDEGLSFSKLILEERMMMAQRLLIYSNHTVGKVAGICGYDNASYFV
                     SVFRGYFGVPPHQYLSRFS"
     gene            5431..5646
                     /locus_tag="IT904_003562"
     CDS             5431..5646
                     /locus_tag="IT904_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP3193295.1"
                     /translation="MKSMDKLTTGIAYSTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGMLTYLTNLYFKIKEDRRKVARRE"
     gene            5651..6202
                     /locus_tag="IT904_003563"
     CDS             5651..6202
                     /locus_tag="IT904_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAP3193296.1"
                     /translation="MTQDYELVVKGVRNFENKVAVTVALRDKKHFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQNVWITYKGKLPG
                     RITGSLKTPPTALRSEKDDIESPIEKLEGSVADLNKKLSVLIPSEDEKKRRDEQFAAF
                     YDYCIEVTRRNFVKIFEEGKSLQ"
     gene            complement(6150..6410)
                     /locus_tag="IT904_003564"
     CDS             complement(6150..6410)
                     /locus_tag="IT904_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193297.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIAASANKLTEEIYPPQKSSQNSCE"
     gene            6524..7057
                     /locus_tag="IT904_003565"
     CDS             6524..7057
                     /locus_tag="IT904_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAP3193298.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            7054..7551
                     /locus_tag="IT904_003566"
     CDS             7054..7551
                     /locus_tag="IT904_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HAP3193299.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KKYQM"
     gene            7915..8127
                     /gene="cspI"
                     /locus_tag="IT904_003567"
     CDS             7915..8127
                     /gene="cspI"
                     /locus_tag="IT904_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HAP3193300.1"
                     /translation="MSNTMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            8138..8326
                     /locus_tag="IT904_003568"
     CDS             8138..8326
                     /locus_tag="IT904_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP3193301.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLSLDLRK"
     gene            8473..8628
                     /locus_tag="IT904_003569"
     CDS             8473..8628
                     /locus_tag="IT904_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193302.1"
                     /translation="MREYPNGETTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAMRLLN"
     gene            8801..8974
                     /locus_tag="IT904_003570"
     CDS             8801..8974
                     /locus_tag="IT904_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAP3193303.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDIKIDLI"
     gene            9268..9474
                     /locus_tag="IT904_003571"
     CDS             9268..9474
                     /locus_tag="IT904_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193304.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            10184..10582
                     /locus_tag="IT904_003572"
                     /pseudo
     CDS             10184..10582
                     /locus_tag="IT904_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003572948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10518..>10708
                     /locus_tag="IT904_003573"
     CDS             10518..>10708
                     /locus_tag="IT904_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193305.1"
                     /translation="MRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSF
                     SALKDRHNAVEVNWIDPNNG"
BASE COUNT         2975 a         2105 c         2547 g         3081 t
ORIGIN      
        1 atccggacct tatctggtca ctacctgatg gagaaatacg tcgctacgac agtcgtctaa
       61 acataatctg tcgcgagtgc cggaagagcg aagttatgca gcgcatactg gcattttatc
      121 agtgaaattt tcgttattta tagacgtaac tagattaaag agcattagtt cagatgtgaa
      181 ttgacatttt cacggcacag gattgagcta gcgtggttgt ctgctttgcg tcaaaagcag
      241 atattaccag atttagacat ctattcccga tagccctgct ctgatgccac actctgtgct
      301 attttcatga ccccaataaa aatatttatg actattgctg atttcaaacg gcctaaattg
      361 gagctcccaa acggggcaaa caaactacta ctgcactctt gctgtgctcc atgttccggt
      421 gaagtgatgg aggcgcttca ggcctcggga atcgactata ccatcttttt ctacaacccg
      481 aacattcatc ctcagaaaga gtatttaatt cgtaaggatg agaatattcg ctttgctgaa
      541 caacacggcg tgccatttat tgatgctgat tacgacacag acaactggtt tgaacgtgcc
      601 aaaggaatgg aatgggaacc cgagaggggg atccgttgta ccatgtgttt tgacatgcgt
      661 tttgagcgga cagcgctgta cgctgctgaa aatggtttca gtgtgatcag cagttcactg
      721 ggcatttcac gctggaaaaa tatgcagcag gttaccgtca ctggataatc tcagtcagat
      781 tgccttactc cgaacgccca ccgcgtccgt cccgtacatt ccagcaacgc atctggaggt
      841 aacgtgcggg tattacttca acctgttctg gtaccggaac tcgggctggt ggtcgttaag
      901 ccgggccgtg aatccatgcc ggtattccac aatacccggg tactggtgga gccggaaccg
      961 aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg ttcgccagcc gctggtggaa
     1021 gacaaaacat tgctgccgtt tttcagtaac gcacgggtaa ttcgtgctgc tggtggtgct
     1081 ggtgcattgt ctgactggct gttgcgccat attaaatcct gccagtggcc acacggcgat
     1141 tatcatcaca gcgaaaccgt tattcaccgt tatggtaccg gcgcaatggt gttgtgctgg
     1201 cactgcgaca accagctgcg tgaccagaca tccgaatcac tcgagcaact tgctcatcaa
     1261 aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac gcaggagcgt
     1321 gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc ggacgcgcta
     1381 ccggaagtgg tattacgtcg ctcgctggga ttaccagcgg aaaaaaactg ctcggtgtac
     1441 cgcgaaagcg acatcgtacc gggagagcag accgccacca gcatattgaa acagcgcaca
     1501 aaaaatcttg caccgttgcc tcacgcccac cagcaacaaa acccatcaca ggaaaagacg
     1561 gtggtaagca tcaccgttga tccagagtct ccggaatctt tcatgaagct gcctaaacgt
     1621 caccgttggg ttaaggagaa atacacacgc tgggttaaga cacagccgtg tgcttgctgc
     1681 ggtatgccag ccgacgatcc gcatcatctg attggtcacg ggcagggcgg aatgggaacc
     1741 aaagcacatg atctctttgt gttgcctttg tgcagaaagc atcacgacga gttgcatacg
     1801 gataccgtgg catttgaaga gaagtatggc tcccaactgg agctgatttt tcgatttatc
     1861 gatcgcgcgc tggcaattgg tgtgctggcc taattttgtg gagtaagttg atgcgtgata
     1921 ttcagatggt tcttgagcgt tggggggcat gggcggctaa taatcatgaa gatgtgacct
     1981 ggtcgtccat tgccgccggt tttaagggat taattccttc aaaagtaaga tctcgtccgc
     2041 aatgctgtga cgatgacgcg atgatcattt gtgaatgtat ggctcgcctg aaaaaaaaca
     2101 acagcgattt acatgattta ttggtggact attatgtcgg cggcatgact tttatggcgc
     2161 ttgcacgtaa gcatgggcga tctgattgtt gggttggcag gatgctccag aaagctgagg
     2221 gcgtagtgga gggcatgctg atggtgttgg atctccgatt ggagatggat gctgattgtt
     2281 cgaaataatt aaaggaaaag ttgctgtctg attttcatta gtctaacatt ttaaatgttg
     2341 gaatcgcaac gtagttatta tcatataaca gcttgtttcc tgatttagcc agcctcccca
     2401 aaggctggtt tttttctaat aagtatcatt tcgggtgggg attttattgt ttaacccata
     2461 atagttcatt gacattgaat cccaactttt gagcggtacg cacatagtct gcttttacat
     2521 tatctggaat agtttgggtc cttgccagaa tccataggta ttctctgttc ggaccactga
     2581 caagagcata cttatactca tcatccagtt tgattacatt atagccacca tagaaggggc
     2641 caaaaaacga aactttcaac gctgcagttt tagtatctcc agtaaagtat gctttacctt
     2701 cgctctcgct ccatttgttt ttcgttggat cgtatccacg gttaagtacg cgaatccctc
     2761 cgtcgttccg tttttcataa gtagcgctga cctgttccag accacgttcg aaccagttct
     2821 cgaggcgagc tatttcatac cattttccga ggtagcggtt ggcgtcaaaa tttgtaatcg
     2881 gctgcacacc tttaggtggt gtcggggcct tacatgctat aagagtgaaa gagagtgcaa
     2941 tgccagtcaa cacaggccat aacttcataa taaatcctgt acttttgata gttgagagta
     3001 agtatgaaag atagatgagt acgaccgatc acttaaagaa ctttcctact atattaggaa
     3061 tagtcaataa cagaaaaatt gtcagtgatg aagccagaaa ggcaatttag tccgtgcact
     3121 acaaagttta tgtgttaatg aattagtcaa gggggaggaa atgataaaaa aacctgtgat
     3181 tgggatcagc ggttgtttgg gcggttctgc tgttcgtttt gatggtggtc acaaaagaga
     3241 tgacttttta atggacaaat tagtggaatg ggtaacattc agaccagtat gtccggaaat
     3301 ggctataggg ctgccagttc cgcgtcctgc tctacgactt gtgcgctcga agcaaggaaa
     3361 tatacggatg tgtttcagcc acgaccagaa tgaggatgtg acagagagaa tgacagagtt
     3421 tagtcgttct tatatggaca aattaaagga tgtatcgggg tttgtggttt gtgctaaatc
     3481 tcccagctgt ggcatggagc gcgtgcgtgt ctatgatgaa aatggtaatc gaggtcgtaa
     3541 agatggagtg ggactattta cgagcacttt gatggaaaag ttttcctggc taccggttga
     3601 agaggatggg cgattacatg atccagtgct tcgtgagaat tttattgaaa gagtttttgc
     3661 tttgcatgag ctcaatcacc tttacaagga gaaattatca agaagagagt tattagcttt
     3721 tcatagtcgt tataagcttc agttgttggc gcatagccag gcaggctata aagatatggg
     3781 accatttgtg gctgcaatac acgagtgggc ggaccttgaa tcatactttg aggtgtatcg
     3841 tgataatctg atggcgattc tcagaaaacc agcatcacgt aaaaatcaca cgaatgtgct
     3901 gatgcatata caggggtatt ttagtaatta cttaagtaca cgccagcgta aagagttgag
     3961 cgaggttata cttaactatc gctttggcac attgcctctt cttgcgccgt tgactctgct
     4021 gaagcattat ctgggtgagt atcctaatga ttacttgctt acacagaatt acttcgatcc
     4081 ctatccggaa gaactggctc taagactgat ggtgaattaa ctgtatgcga tatcatctga
     4141 aagtgatgag ttcctgtatg caggatattt acaatcgtaa aaactacgct atgatgctca
     4201 tagtgtcagt ttttataaaa atcctgttca cgctaaagaa aacattgaga tgcaatttaa
     4261 tgttggtaaa cataccagcc ccaaaagatt ttattcaatc tgtttttata tgaagatatt
     4321 tcaagtgaat ataaagagca tataatatta ttattgcacg cagctattat aatataacag
     4381 attagtttaa tgatttgttt tttgtgagtt aaacacatgg ttttatagtt gtgatgcaat
     4441 gagattttcc ttattgttga actggcgaat attgattttc cacctatact tacctggtgt
     4501 aaccccaatg atatcaggtg gataatatgc catacatatg ttctatcatt ttggtgttga
     4561 actcgtttgg tgtccgaatt ggtaaagaag atattttgtt taaaaaagga aatgctgtcc
     4621 tcattgaata caatttaaaa gatctttttt cgtcaaatac agatcatcta atgatcgtag
     4681 atattgagga gaaaacagtt aatgatttct ttaaaaacta cacactctca cctttttctg
     4741 taagaaggtt ttatccgtca tacttgatag tagaatgtga agatttttca ttgttaagga
     4801 acttggttgc atgcttgaat tgtgatagca aaaccgtgga tgtagttaga aatcaaatat
     4861 cacttgcatg tcttgctgtc ttatcttcag agaaaatagt gcaaagtttt ttgttcggaa
     4921 gtcttaatag cttaggttgt aaagttaagg ctattattca ctcggatatc tcagcgtcat
     4981 ggagactttg tgatatatct tcaagattat atatgagcga gagtttgtta aaaagaaaat
     5041 taaaagacga aggattatcg tttagtaaat taattcttga agaacgaatg atgatggcgc
     5101 aaagattatt gatctacagt aaccatactg tcggcaaggt ggcgggaata tgcgggtatg
     5161 acaatgcatc atattttgta agtgttttta gaggatattt tggtgtgcct ccgcaccaat
     5221 atttatcaag attttcttag aagatatgat gtgatggcat tttataaagt tattttttga
     5281 agtgtaagat tatacgatat ttttagctac gccagaataa ttgctggcgt ttttcttttt
     5341 gaatagatgt tcgggcctta cgctaatgta acttctttcc ttcttcgaac caaaccgtgt
     5401 acaccatccg ttatctgcgg aggtgaggct atgaaatcca tggataagtt aacaacgggc
     5461 atcgcctaca gcacctctgc aggcagtgct ggctactggt ttttacagtt gctcgataaa
     5521 gtcacgccct cacagtgggc ggcaataggt gtattgggta gtctggtatt tggcatgctg
     5581 acgtatctga caaaccttta tttcaagatt aaagaagaca ggcgtaaggt tgcgagaaga
     5641 gagtaatcca atgactcaag actatgaact ggttgtgaaa ggagttcgca attttgagaa
     5701 taaagtggcg gtaactgtag ccttacggga caaaaaacac tttgacggtg aaatttttga
     5761 cctggacatc tcgctggacc gtgttgaagg tgccgcgctg gagttttatg aggcagcagc
     5821 cagaaggagc atcagacagg tcttcctgga tgttgctgcc gggttatgtg aaggggatga
     5881 gcagtcgccg gaaaagcgcc ccataatttt agaggcgcag aatgtgtgga taacctacaa
     5941 aggaaagcta ccgggaagaa ttactggttc tctgaagacg ccaccgacgg cattgcggtc
     6001 agaaaaagat gatattgaat cgcccattga aaaactggag ggcagcgtcg ctgatctgaa
     6061 taaaaaattg tcggtgctga ttccttctga agatgaaaaa aaacgccgcg atgagcagtt
     6121 tgcggcgttt tacgattatt gcattgaagt tactcgcagg aattttgtga agatttttga
     6181 ggagggtaaa tctcttcagt aagcttattg gcggacgctg caattaattc aggaaggtcc
     6241 gcaaggtcat ctgtcagtgg atatgatgaa aaatcggcgg cagttctgtt aagaagcgct
     6301 ttaactaatt ccttttcctt ctccggcaac aagttgatta gagctacgac tgcttgcctg
     6361 agtgcgatta aatcagcaaa agtttgttta ggtagatttg tgtaatccat agtcgcctct
     6421 gtgtttatca gattgacatc ctcctcccgc cagtgcccat cactggtgag gtaagattta
     6481 acatatccgg ggatttgaag ccgataaatc ctgataaata tccatgaacg taaaaatcag
     6541 atacagcctg tcggccgctg ttctggcact gattgccgcc agtgctcctg cccctgacat
     6601 tctcgatcag tttctggatg aaaaagaggg gaatcacacc actgcatacc gcgatggctc
     6661 cggcatatgg accatctgtc ggggagcaac gatggtggac ggtaaacctg ttattccggg
     6721 aatgaaactg tcgaaggaaa aatgcgccca ggttaacgcc attgagcggg ataaggcgct
     6781 ggcatgggtg gagcgcaata taaaagttcc actgaccgag ccacagaaag ccggtatcgc
     6841 gtcattctgt ccctataaca ttggccctgg taagtgtttt ccgtcgacgt tttataagcg
     6901 gcttaatgcc ggtgatcgta aaggcgcatg cgaggcgatt cgctggtgga taaaggacgg
     6961 tggacgtgat tgccgcattc gttcaaataa ttgttacggt caggttattc ggcgtgacca
     7021 ggagagtgca ttaacctgct gggggataga acagtgaatc agatattcac ggtgattttg
     7081 ctcgtgttgg taggatttgt cgtaggtaat gtctggagcg acagaggatg gcaaaaaaaa
     7141 tgggcggagc gtgatgctgc cgaattatct caagaggtaa atgtccaatt tgctgctcga
     7201 ataattgaac aggggcgaac tatatcccgt gatgaggctg ttaaagatgc acaacagaaa
     7261 gccgctgaaa tttctgccag ggctgctgat ctgtctgata gtgttaacca gctgcgtgcc
     7321 gaagcaacaa aatatgccat acgccttgac gcagcgcagc ataccgcaaa tcttgccgct
     7381 gccgtcagag gcaaaacaac caaagccgcc gaaggaatgc tcaccaacat gctcggagat
     7441 attgcagcag aagctcagct ttatgctgaa attgctgaca aacgctacat cgcaggagtg
     7501 acttgtcaaa ggatttatga atctttaaga gataaaaaat atcagatgta gattaatatt
     7561 aaatcggatt atttttagcg ctgaatgtga aatttaaata aaaaggactc ttccatgagt
     7621 caaaatcctt gaaatcttaa gggtaagata aaaggtcatt agacagaatg acacgtttta
     7681 ttaataaata aagctattgt ttcattcgtg tgtttttctt tacaaaagta atccttgcta
     7741 tggttggtta atcatgcgtt aatggtgttc tggtttgtta caaaattatc tgaagcagtc
     7801 attgttataa ttttattatt tgtacctctt gagatttcct tgttggtttt tctctctgat
     7861 attttttttc ggaccattct gcccaagggc taacttcttc aaaaggtaat aatgatgtct
     7921 aacacaatga ctggtttagt gaaatggttt aaccctgaaa aaggttttgg tttcatcacg
     7981 ccgaaagatg gtagcaaaga tgtgtttgtc catttctcag ctattcagag caacgatttc
     8041 aaaacgttaa ctgagaatca ggaagtcgaa tttggtattg agaacggacc taaaggcccc
     8101 gcggctgttc atgttgtggc actttgaggt agaaaatatt acaaaccata ttcactttag
     8161 atgcccgtgt tgccatggtt ctcagtatag aacatcatct tttgatgttt ctgacaggaa
     8221 tcctttcggg gcaaaatgta tcttttgtaa atcaatgatg attacatttg ataatatttc
     8281 acaatactta aatgttagcc gtctatcgtt agatttaaga aagtgaaaat gaaggctcct
     8341 tcgggagctt ttttgcttag tgtctagtcg atggatactc acatattacg gtaacattat
     8401 gaaaaaaata atagtttttt taactctgaa ccagcaatgg tagtgccagt gatgaccgga
     8461 gttaacacca tcatgcgtga atatccaaat ggcgaaacaa cacacctcac tgtaatggct
     8521 gcagggtttc catctctgac cggagatcat aaagtcattt atgtagcagc ggatcgacat
     8581 gtaacttcag aagaaattct ggaagcagca atgagactct tgaattgatt tgattctaat
     8641 gcattgataa taaatgataa tcatctttat ctgtttgtgt gaaatttaga ccgtcgtatg
     8701 ttgattattg cgatgtttca tcttatcttt tatacgtttg ctccatataa ttgacactac
     8761 tgtgtaccag gaaagtcata acagactaaa agaggaaatg atgaacattg aagagttaaa
     8821 aacaaaaaca gaagcagata tttctgaata tataacaaaa aaaatcattg agcttaagaa
     8881 aaagaccggg aaagaagtta ccagtatcca gtttactgca cgggaaaaaa tgactggtct
     8941 tgaaagctat gatatcaaga ttgatttaat ctagtctgtt tagtattagc atcaatttct
     9001 catcagatgc tattcaacaa tacaaattac ccataaacct cgtttttacg gggttttgtt
     9061 atatttaaac attaccgaat aggtataaat ctcgattgtc gatggtattg gttgtgtgac
     9121 aatacctagt ctttccggta tgcctgaaga gaatacaaac gacagattat gtaagggaaa
     9181 cgcgctgaat ctttaactct ttcatgctga gtgatttgac gttatatttt tagccgtaag
     9241 tgaagagcaa acgcatggag cgacaaaatg cataaagatc aatacactga tgccccctcc
     9301 caggagcagg ttcgtgtaaa aactatgctt aatagcacca tttcgatggg ttacccggat
     9361 gttgtaattg catgtataga acatcaagtg tctctggaag cattcagggc aattgaggcc
     9421 gcgttagtaa agcacgataa gaatacgaag gattattccc tggtggttga ctgatcacca
     9481 taactgctaa ccattctgac catttcacaa gtgacagagt caatatcgta ttctgtcact
     9541 gtcaggctaa tacagcaatg caattcaact acagcaatgc ctcgtattta ggtgaattta
     9601 caatatcgtc cagttcggat gccggctgca tttctgaaga taaggcattt cggttctcgt
     9661 attttcccct catgctcgtc agtcctgcgc gtagtaagaa acaggacact cacactaatt
     9721 tgtgtgggca tgctgtgatg tccttctgaa ttatccccat gccattatgt aaagctctgt
     9781 ttcagatgct cgtcacggtt gtcaggctgt cgggtcctcc cagtgggggt ccctgccacg
     9841 gggcgggagc gtcgcggaaa aaggctagtt tttgcatttc catggcggcg gcagcatgtt
     9901 tggtaattta ttgataatta aaaagttatt tctcttttca gctgtacaat atttttttct
     9961 ccctgtcatt agactagttt gcaattaatt gaaatatata aataaacctg attttcacct
    10021 gccagatgga gttgcttatg tcaaatgtga gcgggatcgg tgatgcttat tactggagtg
    10081 tttttaaaat cgccgaggcc tttgggcttc accgggacac agtaaaaaaa cggctcctcg
    10141 cggccaacac tcctgtggct gcgactgtca gggggaaccc cgttaccggt ttgtcgcctt
    10201 aaccgattac gaccgttttc cggagaatat cgacagcgag ggtgatggtt tctcccaggc
    10261 ctcaaaacgt accaccacat ttatgtccgc cgggatgact ctggtggaga gctcgccggg
    10321 acgtgacatc tgcgacagca aatggcgacg taagtcgcct catgaagcgc caccgacgac
    10381 tggtattctt tccctttaca atcgtggtga ccgccgccgc tggtactggc catgtccgca
    10441 ctgcggtgaa tattttcagc cagccatgga tgccatgacc ggctaccgta atgaaccgga
    10501 tccctttaaa gccagtgatg cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg
    10561 tgcaggaccg accatcagat aaggtgtgga cctataaccg cagtaatgtg gtgatgccgg
    10621 atgatggcgc gccgttccgc tacagcttca gcgccctgaa ggaccgccat aatgccgttg
    10681 aggtgaactg gattgacccg aacaacgg
//
LOCUS       DACAPB010000154        10623 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.154, whole genome shotgun sequence.
ACCESSION   DACAPB010000154 DACAPB010000000
VERSION     DACAPB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.154"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(97..1461)
                     /gene="mnmE"
                     /locus_tag="IT904_003574"
     CDS             complement(97..1461)
                     /gene="mnmE"
                     /locus_tag="IT904_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP3193306.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1567..3213)
                     /gene="yidC"
                     /locus_tag="IT904_003575"
     CDS             complement(1567..3213)
                     /gene="yidC"
                     /locus_tag="IT904_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP3193307.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3216..3473)
                     /gene="yidD"
                     /locus_tag="IT904_003576"
     CDS             complement(3216..3473)
                     /gene="yidD"
                     /locus_tag="IT904_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP3193308.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3437..3796)
                     /gene="rnpA"
                     /locus_tag="IT904_003577"
     CDS             complement(3437..3796)
                     /gene="rnpA"
                     /locus_tag="IT904_003577"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP3193309.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3813..3953)
                     /gene="rpmH"
                     /locus_tag="IT904_003578"
     CDS             complement(3813..3953)
                     /gene="rpmH"
                     /locus_tag="IT904_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP3193310.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4183..4263)
                     /locus_tag="IT904_003579"
     CDS             complement(4183..4263)
                     /locus_tag="IT904_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193311.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4560..5963
                     /gene="dnaA"
                     /locus_tag="IT904_003580"
     CDS             4560..5963
                     /gene="dnaA"
                     /locus_tag="IT904_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP3193312.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5968..7068
                     /gene="dnaN"
                     /locus_tag="IT904_003581"
     CDS             5968..7068
                     /gene="dnaN"
                     /locus_tag="IT904_003581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP3193313.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7068..8141
                     /gene="recF"
                     /locus_tag="IT904_003582"
     CDS             7068..8141
                     /gene="recF"
                     /locus_tag="IT904_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP3193314.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8170..10584
                     /gene="gyrB"
                     /locus_tag="IT904_003583"
     CDS             8170..10584
                     /gene="gyrB"
                     /locus_tag="IT904_003583"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP3193315.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
BASE COUNT         2503 a         2795 c         2950 g         2375 t
ORIGIN      
        1 attttaatgt ttaaaaaaag ggttgaggaa taacaggagt ggtaacaatg aagcgaagcc
       61 gcatctgaca gtcagaatgc ggcttcgtaa gcgcggttac ttaccaatac agaagctgga
      121 gaaaatccgc cccagcaggt cgtctgaagt aaattccccg gtgatttcgc ttaagttctg
      181 ctgtgccaga cgcaactctt ccgccagcag ttcacctgcc caggctccca acagttgcgc
      241 tttgccctgt tgtagatgtt ccgctgcctg ttccagcgcc tgtaggtggc gacgacgcgc
      301 caggaagccg ccttccatgt tggtgtcaaa gcccatgctc tgtttgagat ggttacgcag
      361 cacctccacg ccttcacctg tccttgccga gagacgaatt aacgcgtgac cgttcacttc
      421 actcattccc agcgtttcgc cggtgatatc ggctttattg cgcaccacgg tgatcggcag
      481 tttcgctggc agacgggcga taaattccgg ccagatctct gccggatcca cggcgtctgt
      541 tgtggtgcca tcgaccataa acagcacgcg gtcggcctgt tcaatttcct gccacgcgcg
      601 ctcgatacca atacgttcca cttcgtcact ggcttcacgt agcccagcgg tatcgatgat
      661 atgcagcggc attccgtcaa tgtggatatg ctcacgcagc acgtcacgtg tggttccggc
      721 gatatcagtt acgattgctg cttcacggcc cgccagcgcg tttaacaggc tcgatttacc
      781 ggcgttagga cgtccggcaa tcaccacctt catcccttcg cgcaacaaac taccctgacg
      841 tgcttcagca cgcactgcat cgagatcggc aatcacgtca ttaagctggg cttcaatttt
      901 tccgtcggag aggaaatcga tctcttcatc ggggaaatca attgccgctt cgacgtaaat
      961 gcgcaagtgg gtgagggctt ctaccagatg attaacccgt gcggagaatg cgccttgcag
     1021 cgagttaagt gccgaacggg ccgcctgttc cgaactggcg tcgataagat cggcaatcgc
     1081 ctcggcctgg gctaagtcaa gtttatcgtt aagaaacgcg cgttcggaaa actcaccagg
     1141 gcgagcaatc cgcaggccgg gaatggtcag aatgcgtttt aacagcaggt cgaggatcac
     1201 cggaccgcca tgaccttgca gttccagcac atcttcgccg gtgaacgagt tcgggccagg
     1261 gaaccatagc gcaatcccct gatcgagcac gctgccgtcg gcgtctttaa acggaagata
     1321 atcggcgtag cgcggcttag gcagtttacc cagcacggtt tcggcaactt cacgggcttt
     1381 gaagccggag atgcgcagga tgccaacgcc gccacgtccc ggaggcgtgg cctgggctac
     1441 gatagtgtca ttatcgctca tgatgttcct gttgctttgt gtggcggatg cgcggtgctt
     1501 atccgcccta cgaaaagaaa aaaggcggtc aactgaccgc ccttatttta gcgaaaactc
     1561 accgaatcag gattttttct tctcgcggct atgcaggcca cgtttttcca gaccacggta
     1621 aatcagctgc tgctgaataa tggttaccag gttgctgacg atatagtaca gcaccagacc
     1681 tgacgggaac cacaggaaga acacggtgaa gatgaccggc ataaaggtca tgatcttctg
     1741 ctgcatcggg tcggtcacag tggtcggcga catcttctga atgaagaaca tcgttacgcc
     1801 catcaggatc ggcaggatgt agtacgggtc ctgtgccgac aggtcgtgga tccacagtgc
     1861 aaacggtgcc tgacgcagtt caacggaacc catcagcatg tagtacaacg ccaggaagat
     1921 tggcatctgg atcagcagcg ggaagcagcc gcccagcggg ttaaccttct cagctttgta
     1981 cagcgccatc atttcctggc tgatacgctg tttgtcatcg cccagacgct cacgcattgc
     2041 ctgaatcttc ggctgcagca tacgcatctt cgccatggag gtgtactgcg ctttggtcag
     2101 cgggtacatg atgccacgaa cgataaaggt gatgatgata atggagaagc cccagttacc
     2161 cacaaagcta tggatccatt tcagcagttt gaacagcggc tgagagatga accacaacca
     2221 accgtaatca acggtcagat ccaggtgcgg agcaacagct gccattttgt cctggatttc
     2281 cgggccaacc cacagggtgc tgttcatcgc gccagtctga ccaggctgaa ccagtaccgg
     2341 ctgagattta tagccgatag cggcgatgcc gttacccaga ttagcggtat agaagttgtt
     2401 ggtaccgtcg ttatgcggga tccacgccgt cgcgaaatac tgttgcagca ttgccaccca
     2461 accacctttc gaagagatgt tcaggttttc gttatcggca atggtatcga acttgtattt
     2521 ctcgtacttc tcgtcaggcg tggagtacgc cgcgccgcgg aaggtgtgca gtgcgaagtt
     2581 gctgcttccg gtatcgagat gcggtggcag agtgatggat tgcttcaact gaccaaaggt
     2641 ggagatttcc agcggtttct cgccagcgtt ctgcacgttg tagttgacgt tgacagcgta
     2701 atcaccacgt ttcaggacaa acgttttggt aaacgtgttg cctgccgcgt cggtatacgt
     2761 catcggcacc tgcagttcgt tttgaccttc agccagcaca taagcgtctt tttcaacgtt
     2821 atacagcgga cgcgggccgt tagccgggtt atccgggcca tcacgaccgg tcagaccgct
     2881 ctgtgcctga taaataaact gcggtgaagt ttccagcagc tggaacggct gggtagagtt
     2941 cagctctttc gggtaagcag gcagcagagc ttgctcaaca tcaccaccac gggtgttgat
     3001 ggtcagatca agcacgtcgg tcttaaccga gatcagtttc ccctggccac tggccggtac
     3061 gccctggtcg gcggcgctac ccgctgcggt ggtcgttgtc tgcgtggtct gttgggcctg
     3121 aggttgcggg tttttatcct gctcccaggc ttgccagatc atgaaagaca cgaacagcaa
     3181 agcgatgact aaaagattgc gttgcgaatc catcgttagt gttctctggt atcaaatggt
     3241 ccgggcggga cgggatcgtc accaccaggg tgtaaagggt ggcattttaa tacgcgtttc
     3301 accgtcaacc aactgccttt tatcactcca aacctgcgca atgcctcaat tccgtagctt
     3361 gaacaggttg gagtgaaacg acaatgcggc ccgagtagcg gactaatcag gcgttgatag
     3421 acccgaatga gggctatcag gacccgcgag ccaggcgaca gtggcggcgc cataattttt
     3481 ccaacgcttc cgagagagca cggttatcga ggtcggcaac cccttttttc gccaccacca
     3541 cgaaatccat agccgggagt tcatgttggc gcagacggaa gctttcacgc gtcagacgtt
     3601 taatccgatt gcgttcatgg gcgcgtcgaa cgtttttctt ggcgactgta agaccgatac
     3661 ggggatgccc cagcgaattc aggcggccga gaatggtaat ttgcggcgtg ccagcccgtt
     3721 gtggctgctg gaagacgaat gtgaattgac tgggagttaa caagcgtaac tccctgggaa
     3781 atgcgagctt aaccactcag gggttagctt tattacttag aaacggtcag acgagcgcgg
     3841 cctttagcac gacgacgtgc cagaacctga cgaccatttt tagtagccat acgagcacgg
     3901 aagccgtgag aacggttgcg cttcagtaca gacggttgaa aagtgcgttt catggcgatt
     3961 tctacctaaa cttgaataaa ttcaatggct ttattggata tccgccgaaa aatgaaacga
     4021 tggacaccga agccatgggt gattaaagag gccggattgt aataattgta cactccggag
     4081 tcaattctct ttccttattt accgcgcttt tccgcacctt ttcgcaggga aaatgtacga
     4141 cctcacacca gtggaaacca gcatggcgcg ccgggtggag gattatacgg gctgatgggt
     4201 aaagcgcaag gatcgtcctg gatctttatt agatcgatta agccaatttt tgtctatggt
     4261 cattaaattt tccaatatgc ggcgtaaatc gtgcccgcct cgcggcagga tcgtttacac
     4321 ttagcgagtt ctggaaagtc ctgtggataa atcgggaaaa tctgtgagaa acagaagatc
     4381 tcttgcgcag tttaggctat gatccgcggt cccgatcgtt ttgcaggatc ttgatcgggc
     4441 atataaccgc agacagcggt tcgtgcgtca ccctcaagca gggtcttttc gacgtacgtc
     4501 aacaatcatg aatgtttcag ccttagtcat tatcgacttt tgttcgagtg gagtccgccg
     4561 tgtcactttc gctttggcag cagtgtcttg cccgattgca ggatgagtta ccagccacag
     4621 aattcagtat gtggatacgc ccattgcagg cggaactgag cgataacacg ctggccctgt
     4681 acgcgccaaa ccgttttgtc ctcgattggg tacgggacaa gtaccttaat aatatcaatg
     4741 gactgctaac cagtttctgc ggagcggatg ccccacagct gcgttttgaa gtcggcacca
     4801 aaccggtgac gcaaacgcca caagcggcag tgacgagcaa cgtcgcggcc cctgcacagg
     4861 tggcgcaaac gcagccgcaa cgtgctgcgc cttctacgcg ctcaggttgg gataacgtcc
     4921 cggccccggc agaaccgacc tatcgttcta acgtaaacgt caaacacacg tttgataact
     4981 tcgttgaagg taaatctaac caactggcgc gcgcggcggc tcgccaggtg gcggataacc
     5041 ctggcggtgc ctataacccg ttgttccttt atggcggcac gggtctgggt aaaactcacc
     5101 tgctgcatgc ggtgggtaac ggcattatgg cgcgcaagcc gaatgccaaa gtggtttata
     5161 tgcactccga gcgctttgtt caggacatgg ttaaagccct gcaaaacaac gcgatcgaag
     5221 agtttaaacg ctactaccgt tccgtagatg cactgctgat cgacgatatt cagttttttg
     5281 ctaataaaga acgatctcag gaagagtttt tccacacctt caacgccctg ctggaaggta
     5341 atcaacagat cattctcacc tcggatcgct atccgaaaga gatcaacggc gttgaggatc
     5401 gtttgaaatc ccgcttcggt tggggactga ctgtggcgat cgaaccgcca gagctggaaa
     5461 cccgtgtggc gatcctgatg aaaaaggccg acgaaaacga cattcgtttg ccgggcgaag
     5521 tggcgttctt tatcgccaag cgtctacgat ctaacgtacg tgagctggaa ggggcgctga
     5581 accgcgtcat tgccaatgcc aactttaccg gacgggcgat caccatcgac ttcgtgcgtg
     5641 aggcgctgcg cgacttgctg gcattgcagg aaaaactggt caccatcgac aatattcaga
     5701 agacggtggc ggagtactac aagatcaaag tcgcggatct cctttccaag cgtcgatccc
     5761 gctcggtggc gcgtccgcgc cagatggcga tggcgctggc gaaagagctg actaaccaca
     5821 gtctgccgga gattggcgat gcgtttggtg gtcgtgacca cacgacggtg cttcatgcct
     5881 gccgtaagat cgagcagttg cgtgaagaga gccacgatat caaagaagat ttttcaaatt
     5941 taatcagaac attgtcatcg taaacctatg aaatttaccg tagaacgtga gcatttatta
     6001 aaaccgctac aacaggtgag cggtccgtta ggtggtcgtc ctacgctacc gattctcggt
     6061 aatctgctgt tacaggttgc tgacggtacg ttgtcgctga ccggtactga tctcgagatg
     6121 gaaatggtgg cacgtgttgc gctggttcag ccacacgagc caggagcgac gaccgttccg
     6181 gcgcgcaaat tctttgatat ctgccgtggt ctgcctgaag gcgcggaaat tgccgtgcag
     6241 ctggaaggtg aacggatgct ggtacgctcc gggcgtagcc gtttttcgct gtctaccctg
     6301 ccagcggcgg atttcccgaa cctcgatgac tggcagagtg aagtcgaatt taccctgccg
     6361 caggcaacga tgaagcgtct gattgaagcg acccagtttt ctatggcgca tcaggacgtt
     6421 cgctattact taaatggtat gctgtttgaa accgaaggtg aagaactgcg caccgtggca
     6481 accgacggcc accgtctggc ggtctgttca atgccaattg gtcaatcttt gccaagccat
     6541 tcggtgatcg taccgcgtaa aggcgtgatt gaactgatgc gtatgctcga cggcggcgac
     6601 aatccgctgc gcgtacagat tggcagcaac aacattcgcg cccacgttgg cgactttatc
     6661 ttcacctcca aactggtgga tggtcgcttc ccggattatc gccgcgttct gccgaagaac
     6721 ccggacaaac atctggaagc tggctgcgat ctgctcaagc aggcgtttgc tcgcgcggcg
     6781 attctctcta acgagaaatt ccgcggcgta cgtctttatg tcagcgaaaa ccagctgaaa
     6841 atcaccgcca acaacccgga acaggaagaa gcggaagaga tcctcgacgt tacctatagc
     6901 ggtgcggaga tggaaatcgg cttcaacgtc agttatgtgc tggatgttct gaacgcgctg
     6961 aaatgcgaaa acgtccgcat gatgctgacc gattcggttt ccagcgtgca gattgaagat
     7021 gcggccagcc agagcgcggc ttatgttgtc atgccaatga gactgtaatg tccctcaccc
     7081 gcttgttgat ccgcgatttc cgcaacattg aaaccgcgga tctcgcctta tctcccggct
     7141 ttaactttct ggtaggtgcc aacggcagtg gcaaaaccag cgtgctggaa gccatctata
     7201 cgctcggcca tggtcgggcg tttcgcagtt tgcagattgg tcgcgtcatt cgccatgagc
     7261 aggaggcgtt tgttctccac gggcgattac agggcgaaga gcgcgagaca gcgattggct
     7321 taaccaaaga caaacagggc gacagcaaag tccgcatcga cggtacagac gggcataagg
     7381 tcgcggaact ggcgcatctg atgccaatgc agttgataac gccagaaggg tttactttac
     7441 tcaacggcgg ccccaaatac agaagagcat tcctcgactg gggatgcttt cacaacgaac
     7501 ccggattttt caccgcctgg agcaatctca agcgattgct caagcagcgc aatgcggcgc
     7561 tgcgccaggt gacacgttac gaacagctac gcccgtggga taaagagctg atcccgctgg
     7621 cggagcaaat cagcacctgg cgcgcggagt atagcgccgg tatcgcggct gatatggccg
     7681 atacttgtaa gcaatttctc cctgagtttt ctctgacttt ctctttccag cgcggctggg
     7741 agaaagagac agaatatgct gaggtgctgg aacgtaattt tgaacgcgat cgccagctaa
     7801 cctacaccgc gcatggcccg cataaagcgg acttacgcat tcgcgccgac ggtgcgccgg
     7861 tggaagatac cttatcgcgt gggcagctta agctgttgat gtgcgcctta cgtctggcgc
     7921 aaggagagtt cctcacccgt gaaagcgggc ggcggtgtct ctacctgata gatgattttg
     7981 cctctgagct tgatgatgag cgtcgcgggc tgcttgccag ccgcttaaaa gcgacgcaat
     8041 cacaggtctt tgtcagcgcg atcagtgctg aacacgttat agacatgtcg gacgaaaatt
     8101 cgaagatgtt taccgtggaa aagggtaaaa taacggatta acccaagtat aaatgagcga
     8161 gaaacgttga tgtcgaattc ttatgactcc tccagtatca aagtcctgaa agggctggat
     8221 gcggtgcgta agcgcccggg tatgtatatc ggcgacacgg atgacggcac cggtctgcac
     8281 cacatggtat tcgaggtggt agataacgct atcgacgaag cgctcgcggg tcactgtaaa
     8341 gaaattatcg tcaccattca cgccgataac tctgtctctg tacaggatga cgggcgcggc
     8401 attccgaccg gtattcaccc ggaagagggc gtatcggcgg cggaagtgat catgaccgtt
     8461 ctgcacgcag gcggtaaatt tgacgataac tcctataaag tgtccggcgg tctgcacggc
     8521 gttggtgttt cggtagtaaa cgccctgtcg caaaaactgg agctggttat ccagcgcgag
     8581 ggtaaaattc accgtcagat ctacgaacac ggtgtaccgc aggctccgct ggcggttacc
     8641 ggcgagactg aaaaaaccgg caccatggtg cgtttctggc ccagcctcga aaccttcacc
     8701 aatgtgaccg agttcgaata tgaaattctg gcgaaacgtc tgcgtgagtt gtcgttcctc
     8761 aactccggcg tttccattcg tctgcgcgac aagcgtgacg gcaaagaaga ccacttccac
     8821 tatgaaggcg gcatcaaggc gttcgttgaa tatctgaaca agaacaaaac gccgatccat
     8881 ccgaatatct tctacttctc caccgaaaaa gacggtattg gcgtcgaagt ggcgttgcag
     8941 tggaacgatg gcttccagga aaacatctac tgctttacca acaacattcc gcagcgtgac
     9001 ggcggtactc acctggcagg cttccgtgcg gcgatgaccc gtaccctgaa cgcctacatg
     9061 gacaaagaag gctacagcaa aaaagccaaa gtcagcgcca ccggtgacga tgcgcgtgaa
     9121 ggcctgattg cggtcgtttc cgtgaaagtg ccggacccga aattctcctc ccagaccaaa
     9181 gacaaactgg tttcttctga ggtgaaatcg gcggttgaac agcagatgaa cgaactgctg
     9241 gcagaatacc tgctggaaaa cccaaccgac gcgaaaatcg tagttggcaa aattatcgat
     9301 gctgcccgtg cccgtgaagc tgcgcgtcgc gcgcgtgaaa tgacccgccg taaaggtgcg
     9361 ctcgacttag cgggcctgcc gggcaaactg gcagactgcc aagaacgcga tccggcgctt
     9421 tccgaactgt acctggtgga aggggactcc gcgggcggct ctgcgaagca ggggcgtaac
     9481 cgcaagaacc aggcgattct gccgctgaag ggtaaaatcc tcaacgtcga gaaagcgcgc
     9541 ttcgataaga tgctctcttc tcaggaagtg gcgacgctta tcaccgcgct tggctgtggt
     9601 atcggtcgtg acgagtacaa cccggacaaa ctgcgttatc acagcatcat catcatgacc
     9661 gatgcggacg tcgacggctc gcacattcgt acgctgctgt tgaccttctt ctatcgtcag
     9721 atgccggaaa tcgttgaacg cggtcacgtc tacatcgctc agccgccgct gtacaaagtg
     9781 aagaaaggca agcaggaaca gtacattaaa gacgacgaag cgatggatca gtaccagatc
     9841 tctatcgcgc tggacggcgc aacgctgcac accaacgcca gtgcaccggc attggctggc
     9901 gaagcgttag agaaactggt atctgagtac aacgcgacgc agaaaatgat caaccgcatg
     9961 gagcgtcgtt atccgaaagc aatgctgaaa gagcttatct atcagccgac gctgacggaa
    10021 gccgacctct ctgatgagca gaccgttacc cgctgggtga acgcgctggt cagcgaactg
    10081 aacgacaaag aacagcacgg cagccagtgg aagtttgatg tccacaccaa tgccgaacaa
    10141 aacctgttcg agccgattgt tcgcgtgcgt acccacggtg tggatactga ctatccgctg
    10201 gatcacgagt ttatcaccgg tggcgaatat cgtcgtatct gcacgctggg tgagaaactg
    10261 cgtggcttgc tggaagaaga tgcgtttatc gaacgtggcg agcgtcgtca gccggtagcc
    10321 agcttcgagc aggcgctgga ctggctggtg aaagagtccc gtcgcggcct ctccatccag
    10381 cgttataaag gtctgggcga gatgaacccg gaacagctgt gggaaaccac catggacccg
    10441 gaaagccgtc gtatgctgcg cgttaccgtt aaggatgcga ttgctgctga ccagttgttc
    10501 accacgctga tgggcgacgc cgttgaaccg cgccgtgcgt ttattgaaga gaacgccctg
    10561 aaagcggcga atatcgatat ttaatggcgc taaccatgcg agcgtgcctg atgcgctacg
    10621 ctt
//
LOCUS       DACAPB010000155        10504 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.155, whole genome shotgun sequence.
ACCESSION   DACAPB010000155 DACAPB010000000
VERSION     DACAPB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.155"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(114..1283)
                     /locus_tag="IT904_003584"
     CDS             complement(114..1283)
                     /locus_tag="IT904_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3193316.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(1449..1538)
                     /gene="ynhF"
                     /locus_tag="IT904_003585"
     CDS             complement(1449..1538)
                     /gene="ynhF"
                     /locus_tag="IT904_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAP3193317.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            1837..2862
                     /gene="purR"
                     /locus_tag="IT904_003586"
     CDS             1837..2862
                     /gene="purR"
                     /locus_tag="IT904_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAP3193318.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(2859..3791)
                     /locus_tag="IT904_003587"
     CDS             complement(2859..3791)
                     /locus_tag="IT904_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3193319.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            3904..5115
                     /locus_tag="IT904_003588"
     CDS             3904..5115
                     /locus_tag="IT904_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAP3193320.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            5406..6554
                     /gene="cfa"
                     /locus_tag="IT904_003589"
     CDS             5406..6554
                     /gene="cfa"
                     /locus_tag="IT904_003589"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAP3193321.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6594..7235)
                     /gene="ribE"
                     /locus_tag="IT904_003590"
     CDS             complement(6594..7235)
                     /gene="ribE"
                     /locus_tag="IT904_003590"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAP3193322.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            7450..8823
                     /gene="mdtK"
                     /locus_tag="IT904_003591"
     CDS             7450..8823
                     /gene="mdtK"
                     /locus_tag="IT904_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAP3193323.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALAEKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(8864..10120)
                     /locus_tag="IT904_003592"
     CDS             complement(8864..10120)
                     /locus_tag="IT904_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193324.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            10428..10504
                     /locus_tag="IT904_003593"
     tRNA            10428..10504
                     /locus_tag="IT904_003593"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10462..10464,aa:Val,seq:gac)
BASE COUNT         2473 a         2772 c         2541 g         2718 t
ORIGIN      
        1 acgcgtcttt tcatgtcaac gcggatgagt cgttaacacc gcatccggca agataaatcg
       61 cacgttgtca gcaactgtaa cgcagaaggt tatccttctg cgtttttgtt taattagctg
      121 ttagcaacgc aaactgtttc aggttgtttt ctggctgaca taaacaccag caataatgcc
      181 agtcccgcga caatcgctcc catcaccggc acaaagctgt atcccagccc agcggaaatt
      241 accgcaccac cagcagctgc tcccagcgca tttccaagat taaaggcacc aatattgact
      301 gatgaagaca gacccggcgc ttcactggcg acacgcatca cgcgcatctg taacggcggt
      361 acgaccgcaa aggttgctgc gccccacacc accatgctaa tagctgcgcc gaactcattg
      421 cgggccagga acgggattgc cagcataatc accatcagca gcaacaaaaa gcctttcaac
      481 gtgccgttaa ctgaacgatc tgccagtttg ccgccgagat agttaccgat agagaatccg
      541 acaccaatca gcaccagcat tgccgtgacg aacaccggtg ttgcgtgggt aatactttgc
      601 agtaccggag agatataggt gtagagagta aacattgcac cagctcccag taccgtcgtc
      661 agcaatgcag acagcacctg cggacgcatt aataccgcca gctctttttt cacttcaggt
      721 cgtgcccctg caccaccttt aggtaatgag aagaacagac ttaccattga aatcactccc
      781 agccccgccg ttgccagaaa tgacatccgc cagccgatgg tttcacccaa ccaggtcgcc
      841 gccggcacgc caccgatatt tgccagggtt aaccccataa acatagtggc aactgcgctg
      901 gcctgtttat gttttggcac cacgcttgcg gccacgactg aacccaaacc aaaaaatgct
      961 ccgtgattca ggctggtcaa aatgcgtgaa agcatcaggg tcatataatc cggcgcgatg
     1021 gcggaaagaa cgttgcctag cgtgaaaatt gccatcagga aaatcaacgc actgcggcgg
     1081 gcacgatgag aaagtagaag cgtcatcagc ggcgcgccca ccattacgcc aactgcatag
     1141 gcactgatta acattccggc agcgggaatc gagacatcca caccgcgcgc aatgacgggc
     1201 aacaagccca ttggcgagaa ctccgttgtc ccgataccaa acgcgccaat cgccagcgcc
     1261 agcaacggat agttaatttt catgccttat ctccacctct tcgcgtcatt acgcgatatt
     1321 cattaaagtg gcgaaagcat gacagcaatc acaaaaaaat gaaaataaca aaaagagaaa
     1381 acacttttgc cattttgcta acaaacagga aggagatgcg agggagaacg cgctccctcg
     1441 agaggaaatc agtgcagcgc ggcagtcaaa cccacggcta cgatcaaacc gaggacgata
     1501 atcgttgtta ccagtgaaaa tttaaggtcg gtgctcatca agttttctcc ttttttatta
     1561 ccacacaaaa agtgatatta cgcattttta cacactgtga tgaaaaaatc tcccgtcatt
     1621 tataatgata agtgttttta ccacttcccc ttttcgtcaa gatcggccaa aattccacgc
     1681 ttacactatt tgcgtactgg ccattgaccc cttcctgacg ctccgtgtcg tttttccggc
     1741 gtaccgcaac acttttgttg tgcgtaaggt gtgtaaaggc aaacgtttac cttgcgattt
     1801 tgcaggagct gaagttaggg tctggagtga aatggaatgg caacaataaa agatgtagcg
     1861 aaacgagcaa acgtttccac tacaactgtg tcacacgtga tcaacaaaac acgtttcgtc
     1921 gctgaagaaa cgcgcaacgc cgtgtgggca gcgattaaag aattacacta ctcccctagc
     1981 gcggtggcgc gtagcctgaa ggttaaccac accaagtcta tcggtttgct ggcgaccagc
     2041 agcgaagcgg cctattttgc cgagatcatt gaagcggttg aaaaaaattg cttccagaaa
     2101 ggttacaccc tgattctggg taatgcgtgg aacaatcttg agaaacagcg ggcttatctg
     2161 tcgatgatgg cgcaaaaacg cgtcgatggt ctgctggtga tgtgttctga gtacccagag
     2221 ccgttgctgg cgatgctgga agagtatcgc catatcccaa tggtggtgat ggactggggt
     2281 gaagcaaaag ctgacttcac cgatgcggtc attgataacg cgtttgaagg cggctacatg
     2341 gccgggcgtt atctgattga acgcggtcac cgcgaaatcg gcgttatccc cggcccgctg
     2401 gaacgtaaca ctggcgcagg ccgccttgcc ggttttatga aggcgatgga agaagcaatg
     2461 atcaaggtgc cggaaagctg gattgtgcag ggtgattttg aacctgaatc cggttatcgc
     2521 gccatgcagc aaatcctgtc gcagccgcat cgccctactg ccgtcttctg tggtggcgat
     2581 atcatggcaa tgggcgcact ttgtgctgct gatgaaatgg gcctgcgcgt cccgcaggat
     2641 gtttcgctga tcggttatga taacgtgcgc aacgcccgct attttacgcc ggcgctgacc
     2701 acgatccatc agccaaaaga ctcgctgggt gaaacagcgt tcaacatgct gttggatcgt
     2761 atcgtcaaca aacgtgaaga accgcagtcc attgaagtgc atccgcgctt gattgaacgc
     2821 cgctccgtgg ctgacggccc gttccgcgac tatcgtcgtt aatcacccgt tgcgggagtc
     2881 tcttccggct cccgcagcca ctccttattc agcgtctcac tatcgccgag atactcaagc
     2941 aaccaggtta acgcaggcga catatcattt tgctgccatg tcagacaaca tgccgaatcc
     3001 ggaaaggggt tttccagttc taatgctacc cacttcccct cattaagcca cggtttggcg
     3061 aaatgtgttg gcaccatccc tatgcataat cctgccgaga tacaggttgc cgatgattcc
     3121 cagtcaggca cgacaagtct tttttggtta tccagcaacc aggtaatacg cttaggtagc
     3181 gttcgcgagg tgtcttcgcg caccaacgac ggccagttgc gcaacgtatc atcgctgaat
     3241 gggccatcca tcaacgccag cgggtggtgg ctggcaacaa cgcaactcca gcttagcatc
     3301 cccatatccc ggaaggcata acgaccgcct accggaatcg cgcgtgttgc gccaatcgcc
     3361 agttccacgc gcccgtcgga aagcgcatcc cagacaccgt tgaacacttc ctgaaagaca
     3421 agaagttcga catcatcaaa atggcgataa aaatcaacga tcatctgccg tgtacgttct
     3481 ggcctgacaa tattatccac tgcgatagct aactgaccgc gccagccgtt cgctatctgc
     3541 tgacattgct ggcgggtgat ctgcattttt ttgacaacag agcgcccttc tttgagaaac
     3601 cacgctccag cagcggtcag ctccacatca cggtgccgtc gttcaaagag cggcaccgcc
     3661 agccactctt ccagctgacg cacggtatag ctgaccgcag aaggaacgcg atgcagctcc
     3721 tgtgccgcag cgctaaaact accattacgc gctaccgcat caacaacttc gagtgaatat
     3781 tctgaccaca tagtctgcct acaaaatttt tgaaaccagt catcaaatat taccgtttca
     3841 caacactgat ttcactccct acactttgcg gcggtgttta attgagagat ttagagaata
     3901 tacatgcaac ctgggaaaag atttttagtc tggctggcgg gtttgagcgt actcggtttt
     3961 ctggcaaccg atatgtatct gcctgctttc gccgccatac aggccgacct gcaaacgcct
     4021 gcgtctgctg tcagtgccag ccttagtctg ttccttgccg gttttgccgc agcccagctt
     4081 ctgtgggggc cactctccga ccgttatggt cgtaaaccgg tgttattaat cggcctgaca
     4141 atttttgcgt taggtagtct ggggatgctg tgggtagaaa acgccgctac gctgctggta
     4201 ttgcgttttg tacaggctgt gggggtctgc gccgcggcgg ttatctggca agcgttagtg
     4261 acggattatt atccttcaca gaaagtcaac cgtatttttg cgaccatcat gccgctggtg
     4321 ggtctgtctc cggcacttgc tcccctgtta ggaagctggc tgctggtcca tttttcctgg
     4381 caggcgattt tcgccaccct gtttgccatt accgtggtgc tgattctgcc tattttctgg
     4441 ctcaaaccca cgacgaaggc ccgtaacaat agtcaggatg gtctgacctt taccgacctg
     4501 ctacgttcta aaacctatcg cggcaacgtg ctgatatatg cggcctgttc agccagtttt
     4561 tttgcatggc tgaccggttc accgttcatc cttagtgaaa tgggctacag cccggcagtt
     4621 attggtttaa gttatgtccc gcaaactatc gcgtttctga ttggtggtta tggctgtcgc
     4681 gccgcactgc agaaatggca aggcaagcag ttattaccgt ggttgctggt gctgtttgct
     4741 gtcagcgtca ttgcgacctg ggctgcaggc ttcattagcc atgtgtcgct ggtcgaaatc
     4801 ctgatcccat tctgtgtgat ggcgattgcc aatggcgcaa tctaccctat tgttgtcgcc
     4861 caggcgctgc gtcccttccc acacgcaact ggtcgcgccg cagctttgca gaacactcta
     4921 caactgggtc tgtgcttcct cgcaagtctg gtagtttcct ggctgatcag tatcagcacg
     4981 ccattgctca ccaccaccag cgtgatgtta tcaacagtag tgctggtcgc gctgggttac
     5041 atgatgcaac gttgtgaaga agttggctgc cagaatcatg gcaatgccga agtcgctcat
     5101 agcgaatcac actgatctat atcgatatac ttatacttag gctgctaaca aaattttgtt
     5161 gtatgattga aattagcggc ctatactaat ttcgagttgt taaagctacg ataaatatta
     5221 tgtttttacg gggacaggat cgttcccgac tcactatgga tggtcatttc ggcaagggct
     5281 cctcctttcc ctctgttcta cgtcggatta tagactcgcg gttttttctg cgagatttct
     5341 cacaaagccc aaaaagcgtc tacgctgttt taaggttctg atcaccgacc agtgatggag
     5401 aaactatgag ttcatcgtgt atagaagaag tcagcgtacc ggatgacaac tggtaccgta
     5461 tcgccaacga attacttagc cgtgccggta tagccattaa cggttctgcc ccggcggata
     5521 ttcgtgtgaa aaaccccgat ttttttaaac gcgttctgca agaaggctct ttggggttag
     5581 gcgaaagtta tatggatggc tggtgggaat gtgaccgact ggatatgttt tttagcaaag
     5641 tcttacgcgc aggtctcgag aaccaactcc cccatcattt caaagacacg ctgcgtattg
     5701 ccggcgctcg tctcttcaat ctgcagagta aaaaacgtgc ctggatagtc ggcaaagagc
     5761 attacgattt gggtaatgac ttgttcagcc gcatgcttga tcccttcatg caatattcct
     5821 gcgcttactg gaaagatgcc gataatctgg aatctgccca gcaggcgaag ctcaaaatga
     5881 tttgtgaaaa attgcagtta aaaccaggga tgcgcgtact ggatattggc tgcggctggg
     5941 gcggactggc acactacatg gcatctaatt atgacgtaag cgtggtgggc gtcaccattt
     6001 ctgccgaaca gcaaaaaatg gctcaggaac gctgtgaagg cctggatgtc accattttgc
     6061 tgcaagatta tcgtgacctg aacgaccagt ttgatcgtat tgtttctgtg gggatgttcg
     6121 agcacgtcgg accgaaaaat tacgatacct attttgcggt ggtggatcgt aatttgaaac
     6181 cggaaggcat attcctgctc catactatcg gttcgaaaaa aaccgatctg aatgttgatc
     6241 cctggattaa caaatatatt tttccgaacg gttgcctgcc ctctgtacgc cagattgctc
     6301 aatccagcga accccacttt gtgatggaag actggcataa cttcggtgct gattacgata
     6361 ctacgttgat ggcgtggtat gaacgattcc tcgccgcatg gccagaaatt gcggataact
     6421 acagtgaacg ctttaaacga atgtttacct attatctgaa tgcctgtgca ggtgctttcc
     6481 gcgcccgtga tattcagctc tggcaggtcg tgttctcacg cggtgttgaa aacggccttc
     6541 gagtggctcg ctaaaggcta ttctatcgcc ccctctccgg gggcgatttc agatcaggct
     6601 tctgtacctg gttgattcat ggcattttct cgtgccgcca gcacacgttc taccgtatct
     6661 accactgcct gagtttgtgg atcgatttca atgttgacgc gtgcgccaag ttttttcttc
     6721 ccaagagtcg tgcgttccag tgtttccgga attaaatgga cgcaaaaacg cgttggcgtg
     6781 acttcgccga cggtcaggct aataccgtcg atgccaataa atcctttgta cagaatatat
     6841 ttcatcaact gactatcctg gactttaaac cagatctggc gattattttc cgaggttaat
     6901 attttcgcca cttcagcggt ggtcataata tgacctgaca ttaagtgtcc gccaatttca
     6961 tcactgaatt tcgccgcacg ctcaacgttt acccaatccc ccacttttaa atcgccaaga
     7021 ttggtaatgc gtaacgtttc tttcatcagg tcaaaactga catggttgcc gttaatttcc
     7081 gtcacggtca ggcagcaacc gttatgcgcc acggacgcac cggtttccag gccgtccagc
     7141 atgtggtcgg gtaactccac cacatgcgta cgaaaatttg gtttctcgtc aatcgacacc
     7201 agttttgcgg tgccctgtac aatccccgta aacatactta caactcctga aatcagttaa
     7261 gacattctgt tcagcacaat agcaggtggc aaacgccctt accagtgaag gggtaagaat
     7321 gactattttt tcactggaga attaataaat cctcgctaca atagactgaa tttcccctgc
     7381 ttcttctttt tgctgcccat tcaggcggct ttttagtctc tcatataact acaaataaaa
     7441 ggtgttcacg tgcagaagta tatcagtgaa gcgcgtctgt tattagcatt agcaatcccg
     7501 gtgattctcg cgcaaatcgc ccaaactgcg atgggttttg tcgataccgt gatggcgggc
     7561 ggctatagtg ccaccgacat ggcggcggtc gctatcggta cttctatctg gcttccggcg
     7621 atcctctttg gtcacggact gctgctggca ttaacgccgg ttatcgcgca attaaatggt
     7681 tctggtcgac gtgagcgcat tgcgcatcag gtgcgacaag gtttctggct ggcaggtttt
     7741 gtttccgttc tcattatgct ggtgctgtgg aatgcaggtt acattatccg ctccatggaa
     7801 aacatcgatc cggctctggc ggagaaagcc gtgggttatc tgcgtgcgtt gctgtggggc
     7861 gcgccgggat atctgttctt ccaggttgcc cgtaaccaat gtgaaggtct ggcaaaaacc
     7921 aagccgggta tggtaatggg ctttatcggc ctgctggtga acattccggt gaactatatc
     7981 tttatttatg gtcatttcgg tatgcctgag ctcggtggcg ttggttgtgg cgtggctact
     8041 gcggcggtgt attgggtcat gttccttgcc atggtttctt acattaaacg cgcccgctcc
     8101 atgcgcgata tccgtaacga aaaaggcacc gcgaaacccg atcctgcggt tatgaaacga
     8161 ctgattcaac tcggtttgcc gattgcgctg gcactgttct ttgaagtgac actgtttgcc
     8221 gtcgtggctc tgttagtgtc tccgctcggt attgttgatg tcgcaggaca ccagattgcc
     8281 ctgaacttta gttcactgat gtttgtgctt ccaatgtcgc tggcggcagc ggtaactatc
     8341 cgcgtaggtt atcgtctggg tcagggctca acgctggatg cgcaaaccgc tgcgcggacc
     8401 gggcttatgg tgggtgtctg tatggcaacc ctgacggcca tattcacggt ttcactgcgg
     8461 gagcaaatcg ccctgttgta caacgacaat cccgaggttg taacgctggc tgcgcatttg
     8521 atgttgctgg cggcggtata tcagatttct gactcaatcc aggtcattgg cagtgggatt
     8581 ttgcgtggtt ataaagatac gcgttccatt ttctatatca cctttacggc ttactgggtg
     8641 ctgggcttgc caagcggcta tattctggca ctgaccgatc tggtcgttga acctatgggg
     8701 ccagcaggct tctggatagg ctttattatt ggcctgacgt cggcagccat tatgatgatg
     8761 ctgcgtatgc ggttcctgca acgtatgccg tcagccatca ttctgcaacg agcgtcccgc
     8821 taataaagac aaggcgcaac cttcacgggt tgcgcctgta tttttacgca ggctggagcg
     8881 ttgcgccaat cccgtcttcg tctggctgta gtttcagtgc gttacacaga gtttgccgac
     8941 tggcatctgt tatctcaaca tgtatatcac cggtaacttc ccctttcaca ttgatgaagt
     9001 caggtgaaat ttctttcgca ctggtttgca ggcaaatcgc tccctctccg cgcaggcgtt
     9061 tcactttcag ttgcacatgc gttgccgcac cagtgatcag ccacagtttt ccgccattca
     9121 gattgatgtt accaacactg aaagtgacac tgtcaccttt taccgtactg ccgtcgagaa
     9181 tcaacacacc gccctgctgt acagtaatcg ttgattgttg cagctctgcg ccataaccaa
     9241 taatcattga agcgccgtcc agtaatgcaa cactcccggt cagttcaacc ggtgcgcgat
     9301 cttcctctac gacaaaattt tcgtccgcgc tggtgggtga caacaggatc actgttccac
     9361 cttgtattac cgcattttcc agcgtaccgg catgcacttc agcccgacca cccacattca
     9421 cggacaggtt ttgcgtctta ccggaactgt agagctgaag gccatccgtc cccagacgat
     9481 aaatggatcc attctcaaca atagtgtcag agtcttcacc accgagatta ttcatggtgc
     9541 caccagcaag caacgtatca ctggctttcc cggccagcat aaaaacgccg ccctgattaa
     9601 tctcacaatt gctggcttcc ccacctggat acaccaacaa ttcaccacct tcatctacca
     9661 cgaccgctga ggttgtccca ttaaccaaca ggccgccctc ttgatcgaga atgatttttt
     9721 ccgcgcgatg gccttccagt acacgcaggt taccgccatt ttccagcaac aaaccgcagg
     9781 cataaccttt atcaacgctg aattcacctt cgggatggcg gccattaacc gtagcgagcg
     9841 tagagagact aattgcgcct ccctcggact gaacaatatc tgtggcaatg gcaccgtcgc
     9901 tgaccgatag cgtgccattg ttaccgtact gtgtgcattt ggcgatccca ccggttgcca
     9961 ctttcaacca gccagccccc gtgaccacgg tattttcggc tcttccgcca gcttcaacaa
    10021 tcaactcgcc ttcagtaagt tgcgtcgcgc caatgacctc gccggtctta acaatttgca
    10081 cattcccgtc gctcatcaag tttttcgcat cagatcccat gatttattcc tttgctgcat
    10141 ctgtgtgcct ttattgctac ctaagtgtaa aggctacgga ggatttatcc acgacagatt
    10201 tgagatggtg gcaaacaact ctgtttaaac tctgatacac gaattattgg gttgtatcag
    10261 atgtaaatgc gatcctgaat aaaaatcgcc cttgcaaatc aacaaaatat agccaatttg
    10321 cttaaaagct ctgcaaacgg tcgaaatagc gcagaaaatt acgttttgcc tcttgccacc
    10381 ttcccactct gccgctaata ttcgtccccg ttgtcaccta caacgttgcg ttcatagctc
    10441 agttggttag agcaccacct tgacatggtg ggggtcgttg gttcgagtcc aattgaacgc
    10501 acca
//
LOCUS       DACAPB010000156        10441 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.156, whole genome shotgun sequence.
ACCESSION   DACAPB010000156 DACAPB010000000
VERSION     DACAPB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.156"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..570
                     /locus_tag="IT904_003594"
     CDS             <1..570
                     /locus_tag="IT904_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP3193325.1"
                     /translation="NPLDALIPSLKAKKIDAIMSSLSITEKRQQEIAFTDKLYAADSR
                     LVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIVSYQGQDNIYSDLT
                     AGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTGMGLRKEDNELREA
                     LNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            660..1346
                     /locus_tag="IT904_003595"
     CDS             660..1346
                     /locus_tag="IT904_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP3193326.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            1343..2059
                     /locus_tag="IT904_003596"
     CDS             1343..2059
                     /locus_tag="IT904_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3193327.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            2067..2840
                     /gene="hisP"
                     /locus_tag="IT904_003597"
     CDS             2067..2840
                     /gene="hisP"
                     /locus_tag="IT904_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAP3193328.1"
                     /translation="MSENKLNVIDLHKRYGKHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            3037..3927
                     /locus_tag="IT904_003598"
                     /pseudo
     CDS             3037..3927
                     /locus_tag="IT904_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(3975..4868)
                     /locus_tag="IT904_003599"
     CDS             complement(3975..4868)
                     /locus_tag="IT904_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAP3193329.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQELADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(4889..5251)
                     /gene="folX"
                     /locus_tag="IT904_003600"
     CDS             complement(4889..5251)
                     /gene="folX"
                     /locus_tag="IT904_003600"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAP3193330.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            complement(5308..5955)
                     /gene="yfcG"
                     /locus_tag="IT904_003601"
     CDS             complement(5308..5955)
                     /gene="yfcG"
                     /locus_tag="IT904_003601"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAP3193331.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            6091..6735
                     /gene="yfcF"
                     /locus_tag="IT904_003602"
     CDS             6091..6735
                     /gene="yfcF"
                     /locus_tag="IT904_003602"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAP3193332.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIVEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            6791..7342
                     /gene="yfcE"
                     /locus_tag="IT904_003603"
     CDS             6791..7342
                     /gene="yfcE"
                     /locus_tag="IT904_003603"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAP3193333.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            7400..7942
                     /gene="yfcD"
                     /locus_tag="IT904_003604"
     CDS             7400..7942
                     /gene="yfcD"
                     /locus_tag="IT904_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAP3193334.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(7975..9495)
                     /gene="yfcC"
                     /locus_tag="IT904_003605"
     CDS             complement(7975..9495)
                     /gene="yfcC"
                     /locus_tag="IT904_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP3193335.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(9685..>10441)
                     /gene="pta"
                     /locus_tag="IT904_003606"
     CDS             complement(9685..>10441)
                     /gene="pta"
                     /locus_tag="IT904_003606"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP3193336.1"
                     /translation="VRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVD
                     GLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQ
                     LAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDG
                     PLQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQ
                     GMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
BASE COUNT         2620 a         2579 c         2743 g         2499 t
ORIGIN      
        1 aatccgctgg atgcgttaat cccgtcctta aaagcgaaga agattgacgc catcatgtca
       61 tcgctttcca ttacggaaaa acgtcagcaa gaaatagcct tcaccgacaa actgtacgct
      121 gccgattctc gtttggtggt ggcgaaaaat tctgacattc agccgacagt cgagtcgctg
      181 aaaggcaaac gggtaggcgt attgcagggc accacccagg agacgttcgg taatgaacat
      241 tgggcaccaa aaggcattga aatcgtctcg tatcaggggc aggacaacat ttattctgac
      301 ctgactgccg gacgtattga tgccgcgttc caggatgagg tcgccgccag cgaaggtttc
      361 ctcaaacaac ctgtcggtaa agattacaaa ttcggtggcc cgtctgttaa agatgaaaaa
      421 ctgtttggcg tagggaccgg catgggcctg cgtaaagaag ataacgaact gcgcgaagca
      481 ctgaacaaag cctttgccga aatgcgcgct gacggtactt acgagaaatt agcgaaaaag
      541 tacttcgatt ttgatgttta tggtggctaa tcgccatcgg tgaaagaaag cccgctccct
      601 tcgggtaacc ggagggagac aagaggcggt aattcaccac acacgacagg acaggcagca
      661 tgttgtatgg gttttcaggt gttattttac agggtgcgct cgtcacgctg gagctggcta
      721 tcagctctgt agtgctcgct gtaatcatcg gtttaattgg cgctggcggt aagctctcgc
      781 aaaatcggct ttcggggctg attttcgaag ggtacaccac gctgattcgt ggcgtgccgg
      841 acttagtgtt gatgctgctg attttctacg gtttgcagat tgcgctaaac acggtgacgg
      901 aggcgatggg cgtcgggcag attgatatcg atccgatggt cgctggtatt atcactctcg
      961 gttttatcta cggtgcttat tttaccgaaa cgttccgtgg tgcttttatg gcagtgccga
     1021 aaggacatat agaggcggcg acggcgttcg gttttactcg tgggcaagtg tttcggcgga
     1081 tcatgtttcc ggcgatgatg cgttacgccc tgccaggcat tggcaacaac tggcaggtga
     1141 tcctcaaatc taccgcactg gtttcgttac tcggcctgga agatgtggtc aaagccaccc
     1201 aactggcagg caaaagtacc tgggaaccgt tctatttcgc catcgtctgt ggcgtgattt
     1261 acctggtttt caccactgtt tccaatggtg tgctgctgtt ccttgagcgc cgctactccg
     1321 tgggtgtgaa gagggctgac ctgtgatcga aatcttacat gaatactgga aaccgctgct
     1381 gtggaccgac ggttatcgct ttactggtgt ggcgatcact ctgtggctgc ttattttgtc
     1441 ggtagtgata ggcggagtcc tggcgctgtt tctggcgatt ggtcgtgtct ccagtaataa
     1501 atacatccag tttccaatct ggttatttac ctatattttt cgcggtacgc cgctgtatgt
     1561 tcagttgctg gtgttctatt ccggcatgta cacgcttgag attgttaagg gaaccgaatt
     1621 ccttaacgct ttcttccgca gtggcctgaa ctgtaccgtg ctggcgctga cgcttaacac
     1681 ctgcgcttac actaccgaga tttttgctgg ggcaatccgt tcggttccgc atggggaaat
     1741 tgaagccgcc agagcctatg gcttctcgac ttttaaaatg tatcgttgca ttattttgcc
     1801 ttctgcgctg cgtattgcgt taccggcata cagcaacgaa gtgatcctga tgctgcactc
     1861 tactgcgctg gcgtttacgg ccactgtgcc ggatctgctg aaaatagccc gcgatattaa
     1921 cgccgccacg tatcaacctt ttaccgcctt cggcattgcc gcggtgctct atttaatcat
     1981 ctcttatgtc ctgatcagcc tctttcgcag agcggaaaaa cgctggttgc agcatgtgaa
     2041 accttcttca acgcactgag aatacgatgt ccgagaataa attaaacgtt atcgatttgc
     2101 acaaacgcta cggcaaacat gaagtgctga aaggggtctc actgcaagcg aatgccggag
     2161 atgtaataag catcatcgga tcgtcgggat cggggaaaag tacctttctg cgctgcatta
     2221 acttcctcga aaaaccgagt gaagggtcga tcgtggtcaa tggccagacg atcaatctgg
     2281 tgcgcgacaa agacggtcaa ctcaaagtcg ccgataaaaa tcaactgcgc ttactgcgca
     2341 cacgcctgac gatggtattc cagcacttca atctctggag ccatatgacg gtgctggaaa
     2401 acgtcatgga agcgccgatt caggtgttgg gcctgagcaa gcaggaagcg cgcgagcggg
     2461 cggtgaagta tctggcgaaa gtcgggatag acgaacgtgc gcaggggaaa tatccggtgc
     2521 atctttccgg cggtcagcaa cagcgtgttt ctatcgcgcg ggcgctggcg atggaaccgg
     2581 aagttttact gtttgatgaa cctacctcgg cgctcgatcc tgaactggta ggcgaagtgt
     2641 tacgtattat gcagcaactg gcagaagagg ggaaaaccat ggtggtagtg actcacgaaa
     2701 tgggctttgc tcgccatgtt tctactcacg tcattttcct ccatcagggg aaaatagaag
     2761 aagagggcgc gccggagcag ttatttggca acccgcaaag ccctcgtctg caacagttcc
     2821 ttaagggatc gctgaaataa tttcctctcc ggtggctaac gctgccggag tattttcatg
     2881 gggaatttgc gagtcagctt ccgctgaaat gtaaaagttg cagtccgcta aaaatacttc
     2941 tgaattcgct gtccaaagat aaaaactgcc tgctgcattg ggtgttaatc gtggttattg
     3001 tcgcgtcatc ttaaatttga ggggtgacgg aacgccatga caatatcgac aacttccacg
     3061 ccgcatgatg cggtatttaa atctttttga cgccatccag acaccgcgcg ggattttatt
     3121 gatattcatc ttcccgcgcc gctgcgcaaa ctgtgtgatt taacgacgct taaactggaa
     3181 ccaaacagtt ttattgatga cgacctgcgg caatattatt ccgacctctt gtggtctgtg
     3241 aaaacgcagg agggagtggg ttatatttat gtagtgatag agcaccaaag taagccggaa
     3301 gaattaatgg cttttcgcat gatgcgttat tccattgcgg caatgcaaaa ccatcttgat
     3361 gcgggctata aagagcttcc attggtgatc ccgatgctgt tttatcatga ttgcagaagt
     3421 ccttatcctt attcactctg ctggcttgat gaatttgccg agcctgctat agcccgcaaa
     3481 atatattcat cggcttttcc gttggtggat attaccgtgg tgccggatga cgagattatg
     3541 caacatcgca aaatggcgct gttggagtta attcagaaac atattcgtca gcgcgatctg
     3601 ttgggattag tcgaccaaat tgtttcgctg ctagttacag ggaacactaa tgacagacag
     3661 ctaaaagccc tgtttaatta cgtattacaa acaggggatg cccagcgttt tcgtgcattt
     3721 attggtgaga tagcggaacg cgcaccacaa gaaaaggaga aactgatgac cattgctgac
     3781 agattacgtg aagaaggcgc aatgcagggc aaacacgaag aagccctgcg tattgctcag
     3841 gagatgctgg atagaggttt agacagagag ttagttatga tggtgacccg actttcacca
     3901 gacgatctta tcgcgcaaag ccactaatcc tgtaacaccg ggagttaact ggcggatgtt
     3961 tgctgtaaac cacatcagcg aacgacatcc gccagcgcct cttctaaatc gtaccagcga
     4021 aacgcaaaac ccgcttcttc cagccgttta ggcagcgcgc gttgtccacc taataccagt
     4081 actgaagatt cgcccattaa cagtcgaatg gcggtcgcgg ggacgcgcaa aatggccggg
     4141 cgatgcagcg catgaccgag cgcatgggca aattgttcat tgcgtaccgg gtagggcgaa
     4201 accatattaa atggcccgcg cagctcgtta tccagcagcc agagaatacc gttgaccata
     4261 tcatcaatat gaatccaggc cagatactgc cgaccagagc caatcggccc gcccaggcca
     4321 agacgaaacg gcggcagcat tttaccgaga ataccgccat ccggtgccag cactacaccg
     4381 gtacgcagca ggcacactcg cgttttgtca ctttgcgccc ggcaggcaat ttcttcccag
     4441 cgggcgcaga gtttatgggt aaattcgtta tgcggcggtt cctcttcggt aaccaccact
     4501 tcacctaagt caccataata gcccgttgcc gagccggaaa tgagtaccga cggtggcgtg
     4561 tcgctggcat taatcaaatc gaccagtttt tgcgtgatat tccagcggct ttggcagaga
     4621 cgctcttttt gctcgtgagt ccagcgttta tcagcaatcg gttctccggc caggttgatt
     4681 accgcatcaa cgccgttgag gttgctttga tcggcaagct cttgccatag tgtcacccga
     4741 gggccgagaa cggaactggc tttctgcggg ttacgcgtca ctaccgtaat ttgatggccc
     4801 agctccagca aacgcggaat caaatggcga ccaattaatc ccgtccctcc ggtgatcact
     4861 atgttcatgc agcctcccga tatggcgatt aacgctgcca gcttaaggtc atggataccg
     4921 aatcggcgta gcgcagcgcg tgcagtttat cgatctccac ttcagcatac gtcacccagt
     4981 gatgttcacg tgcgatatcg agcacatcct gagttaattt ttccagcaaa gagaaacggt
     5041 tattctctac atgctgaata atgtttttcg ttacggtgcg ataattcagc gcatcgttga
     5101 tatcttcgct agtgcgcgct ttatcggcgg ggtagtggat cgtcacattg ataacaatat
     5161 cctgacggtt gttaatttct tcttccttaa ttccgataaa cgtacgcaaa cgaaggttct
     5221 ttatacgaat aatagcggca ggttgtgcca ttgcaggttc tccgttgttt tacttaggat
     5281 gaaataatac acgagaacct gtttctgtta actatccgaa cgctcatcac cgagttgtgc
     5341 ttttagcagt gcctgcccgg tggcagggcg cgaacggatc cgctcatgcc agttcttgac
     5401 tgccggatac attgctaggt caattcgctg gcgagtccag gcattaaccc acggccagca
     5461 ggcaatatcc gcaatgctgt agttctcgcc tcccagccag ggcgagtttt ccagccgctt
     5521 gttcagtaca tggtaaagac gctgagtttc aacctgataa cgttcaatag cgtaaggaat
     5581 ggtttggggg gctgcgtgat taaaatgatg attttgccca agcatcggcc ccagtccgcc
     5641 tacctgccag aataaccact gtaatgtggc ggcgcgctca cgcgtttcat gactcaaaaa
     5701 gagtcctgtt ttctcagcca gatacaacaa aatggcacca gactcaaaga ggcttagcgg
     5761 ttcgccgcca tcggcaggag aatgatcaac aattgccgga attttgttgt tgggcgaaat
     5821 gcgcaaaaat tccggacgaa actgaccgcc tttccccagg tctaccttaa tcaagcgata
     5881 atcaagctct gcttcttcga gaaacagcgt aattttgtga ccattgggtg tcggggcgaa
     5941 atagagatcg atcattgcag ctccggttgt ttaatcatct gaacaccaca agtatagaaa
     6001 gcgcggcgac ttacgtgagt attcgtcacg tgacagtgac aaaaaggctc tatagtttga
     6061 tagccagccc cctttattgc cgaggacata atgagtaaac ccgctatcac gctttggtca
     6121 gatgcccact ttttctcccc ttatgtgtta tccgcctggg tggcgttgca ggaaaaaggc
     6181 ctgtcgtttc atatcaagac catcgacctc gacagcggtg aacatttgca gccgacgtgg
     6241 caaggttacg gtcagacacg ccgtgtgccg ttattacaaa tcgatgattt tgagttgagt
     6301 gaatcttctg ccattgtgga gtatctggaa gatcgatttg cgccaccgac ctgggaacgt
     6361 atttatccgc ttgatttaga aaatcgtgca cgtgcacgac agattcaggc ctggttgcgc
     6421 agcgatctga tgcccatccg cgaagagcgt ccgacggatg ttgtctttgc gggggcgaaa
     6481 aaagcgccac taacggccga gggaaaagcc agtgcagaga aactgttcgc gatggcagaa
     6541 catttgttag tactgggtca gccgaattta tttggtgaat ggtgcattgc tgatactgat
     6601 ctggcgctaa tgattaaccg cctggtacta catggcgatg aggtgccaga acgcctggtg
     6661 gattatgcaa cattccagtg gcagcgagcg tctgtccagc gttttattgc actttcggcg
     6721 aagcaatctg gctgatagca acgacagaat ccagtatcat agcttacgtt ttttcgacga
     6781 ggagtgagtg atgaaactga tgtttgcatc ggacattcat gggtcgttac cggcgacgga
     6841 acgtgttctg gagttgtttg cccaaagcgg tgcccagtgg ctggtgatcc ttggcgacgt
     6901 gttgaatcac ggcccgcgta atgctttacc ggagggttac gcgccagcca aagtcgctga
     6961 acggcttaat gaagtggcac ataaggttat cgctgtgcgc ggcaactgcg acagcgaagt
     7021 ggatcaaatg ctgctgcatt tcccgataac cgcgccgtgg caacaggtat tactggaaaa
     7081 acaacgtctg tttttgacgc atggtcatct ttttggcccg gaaaatctgc ctgctttaaa
     7141 ccagaacgat gtgctggtgt acggtcatac ccatctgcca gtggcagaac agcgggggga
     7201 gattttccac ttcaaccccg gctcggtgag tattccgaaa ggcggtaatc cggcgagtta
     7261 tggcatgctg gataatgacg tacttagtgt aatcgcactc aatgatcaaa gtatcattgc
     7321 gcaggtcgcg attaatccgt aatttaccca caactcaaaa tgcgccgtaa gagcgccaga
     7381 aaaagaaggt ttcctgataa tggaacagcg tcgtttggca agtactgaat gggtggatat
     7441 tgtcaatgaa gagaacgaag tcattgcaca agccagccgg gaacaaatgc gggcacagtg
     7501 tctgcgtcat cgtgcaactt acatcgtcgt gcatgatggc atgggcaaaa ttctggtcca
     7561 gcgtcgtacc gagacaaaag actttttacc cggcatgtta gatgcgaccg caggcggtgt
     7621 agtccaggcc gatgagcaac tgctggaatc cgcgcgtcgc gaagcggaag aagagttggg
     7681 cattgccggt gtcccctttg ccgagcacgg gcagttctat ttcgaagata aaaattgccg
     7741 tgtctggggc gcattgttca gctgcgtctc tcacggtccc ttcgcactac aggaagatga
     7801 agtcagtgaa gtttgctggc tgacgccgga agaaatcacc gcacgctgcg atgagttcac
     7861 tccagactcg ctgaaagcgc tagcgttgtg gatgaagcgc aatgccaaaa atgaagccgt
     7921 agagactgaa acggcagaat gaaaaaagag ccgcaattgc ggctcttaac atttttagtg
     7981 gtagcccatc aactgagcgc cgatcactac gacgctggac ataataaaca gcagtccaag
     8041 tagggtcgcg cccaccttca gccagttacg gaagtccacc ctgcacacac cgagcgttgc
     8101 cattaacgac gctgaggttg ggtaaatgat gtgactgaag ccatcaccaa actggaatgc
     8161 cagcacggta acctgacggt taacaccgac cagatcgcca agcggtgcca gtaacggcat
     8221 ggttaacgcc gcctgaccag aaccggacgt cacgaagaaa ttaaataccg cctggaagag
     8281 caacataaac caggccgcga ccgcgttgtc cagaccgcta atggcattgg caatgctgtt
     8341 gaggatggta tttaacacgc tggcatcacc cgcttcacca ttaccgacca gcagcaaaat
     8401 ccctttggcg aaacccacca gcagggcagg ggcgatcatc attcgcgccc cttcggtaaa
     8461 ggatgaagcc atggtattaa ccgtcatgcc gttaaggcgg aaaacaacgc cgatgatgcc
     8521 aatcaccaga cccatggtga agaactggct ggcaatttct ggaataaacc aggcattaac
     8581 gatcacgccc caaatcaccc agaccattac ggcggtcagg acaatcaata ccagccagtc
     8641 accaaaggta aacggacgtt gttcaacatc cgcctgcttt tcacgaaaga agcggtcgga
     8701 ctcatgcacg cgtgacagaa gaggattctt tttcactcgt gaggcgtaca ccatggtaaa
     8761 gatcaggcca atcatagtgg cgataaccca caccacgatg cgcaacccgg agccagaaag
     8821 caccggaacg ccggcaatcc cctgagcaac gaccacacaa aacgggttca tccacgaact
     8881 ggcaaaaccg atttgcgtgg caatataggt caccaggacg gtggtaatac tgtcatagcc
     8941 cagccggacc attagcggtg cgatgataat ggcaaaggcg acggcctctt ctcccatacc
     9001 aaataccgcg ccgccaagtg aaaacagaat aaacagcgca ggaataaaga gaatttcatt
     9061 cccgcgggta tggcgaataa gcgccaggat accgttatca atggttcctg tacgcatcac
     9121 aatgccaaac gcgccgccaa tcaccagcat aaacatgatg atgccaacag ctgtcccgta
     9181 tttcgatcct gaggttaatc cttcaaacgg gaagttcatc aggcccgggc gttcatcgcc
     9241 cgtcgtgaac agctgtacgc ggtgatactc aggttcgcct gcttcgttag tcagaatgcg
     9301 aaatgagtgt ggatctacga ctttgcgtgt ttttgtttga ccatcaacct gatactgcac
     9361 ttcctgactg tcaaacatcc ccaccggaac tacccaggtg gcaaggctgg ttaaaatagc
     9421 aacaaaaaag ataatcacca acgtatcggg cattgcccat cttcttgttg gtttggattc
     9481 agtgattgcg gacatagcgc aaatattccc ttgcacaaaa caaagtggta agtatgcaaa
     9541 gtgaaatggc gcaattcatt gatgcagcgc agttaagcaa gataatcaga aaggattaat
     9601 gcagattaag agaataaaaa accggaaata gtgattattt ccggttcaga tatccgcagc
     9661 gcaaagctgc ggatgatgac gagattactg ctgctgtgca gactgaatcg cagtcagcgc
     9721 gatggtgtag acgatatcgt caaccagtgc gccacgggac aggtcgttaa ccggcttgcg
     9781 cataccctgc agcatcggcc cgatggagat caggtcggca gaacgctgta ccgctttgta
     9841 ggtggtgtta ccggtgttca gatccgggaa gatgaacacg gtagcgcgac ctgcaaccgg
     9901 agagttcggc gctttggatt tcgcaacgtc agccattacc gcagcgtcgt actgcagcgg
     9961 accgtcgatc atcaggtcag gacgtttttc ctgcgccaga cgagttgctt cgcgaacttt
    10021 ttctacgtcg ctacctgcac cagaagtacc ggtggagtag gagagcatag caacgcgcgg
    10081 ttcgataccg aaggccgcag cggaatcagc ggactgaatc gcgatttctg ccagctgttc
    10141 agcggtcgga tccgggttga tcgcacagtc accgtaaacg taaacctgtt ccggcagcag
    10201 catgaagaac acggaagata ccagggagct gcccggtgca gttttgatca gctgcagcgg
    10261 cggacggatg gtgtttgcgg tagtgtgaac agcaccggaa accagaccat caacttcatc
    10321 ctgttccagc atcagcgtac cgagcaccac gttgtcttcc agctgttcgc gggcaacggt
    10381 ttcggtcatg cctttgttct tacgcagttc gaccagacga ccaacatagc tttcgcgaac
    10441 c
//
LOCUS       DACAPB010000157        10417 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.157, whole genome shotgun sequence.
ACCESSION   DACAPB010000157 DACAPB010000000
VERSION     DACAPB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.157"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1023)
                     /locus_tag="IT904_003607"
     CDS             complement(<1..1023)
                     /locus_tag="IT904_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP3193337.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSTALNMAGAFAAAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQVSQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNESGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGLAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLR"
     gene            complement(1070..1330)
                     /locus_tag="IT904_003608"
     CDS             complement(1070..1330)
                     /locus_tag="IT904_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAP3193338.1"
                     /translation="MGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRAAQIVSA
                     VYGAQGVKVPLNDALLQWEKEQTEGVSDPFAGLEKALLIVSQ"
     gene            complement(1372..1758)
                     /locus_tag="IT904_003609"
     CDS             complement(1372..1758)
                     /locus_tag="IT904_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193339.1"
                     /translation="MRIDVMTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAE
                     AWLRWQEIVKAKDNETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIA
                     VYGPVHARLLRQSLELISDAGEVKKK"
     gene            complement(1758..2462)
                     /locus_tag="IT904_003610"
     CDS             complement(1758..2462)
                     /locus_tag="IT904_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193340.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTASLKVKVDLKSTLRVA
                     SGAKLEMAVEAAGGVPPYSYVWKKGGSPVSGQTAATFSKASASSGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(2522..2866)
                     /locus_tag="IT904_003611"
     CDS             complement(2522..2866)
                     /locus_tag="IT904_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAP3193341.1"
                     /translation="MREATLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TVSDVLDGQAESRITVQIDVWATVPDDADNIREQALDAVRKLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(2863..3312)
                     /locus_tag="IT904_003612"
     CDS             complement(2863..3312)
                     /locus_tag="IT904_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP3193342.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAF
                     YWRFVELGTINMPAHPFIRPAFDTTEELAAQVAIQRMNQAIDEVLSK"
     gene            complement(3309..3647)
                     /locus_tag="IT904_003613"
     CDS             complement(3309..3647)
                     /locus_tag="IT904_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP3193343.1"
                     /translation="MQAGRLRDRVIILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     REIISGGAETAQSTVRVWMRFRRDVTATSRLKVLTGAFKGAILGIEGPPIPDARATRL
                     EILCSLKGNV"
     gene            complement(3656..3973)
                     /locus_tag="IT904_003614"
     CDS             complement(3656..3973)
                     /locus_tag="IT904_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP3193344.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            complement(4050..5267)
                     /locus_tag="IT904_003615"
     CDS             complement(4050..5267)
                     /locus_tag="IT904_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP3193345.1"
                     /translation="MKLHEMKQKRNTIAKDMRALHEKIGDNAWTDEQRAEWNRAKAEL
                     DALDEQIAREEELRRQDQAYVDESGPEERQNNEAENGKKAVEEKRAAAFNRFLRAGFA
                     ELNAEERNLMRELRAQSVTTDSQGGYTVPTQMRNKIIDTMKAYGGIASVAQLLTTSTG
                     QDITWSTSDGTTEEGELLAENTAATEQDVTFGTAILGAKKLSSKIIRVSNELLQDSGV
                     DIESYLANRIAQRIGRGEAKYLVQGTGTGSPLQPKGLAASVTGTIQTAASAAFTWKEM
                     NALKHAIDPAYRGGPKYRWAFNDATLQTIEEMEDGQKRPLWLPDIAGGTPATVLGIPY
                     VIDQAIDGIGTGKKFIFLGDFNRFIIRRVTYMELKRLVERYAEFDQVAFLAFHRFDCV
                     LEDVAAIKALTGK"
     gene            complement(5282..5881)
                     /locus_tag="IT904_003616"
     CDS             complement(5282..5881)
                     /locus_tag="IT904_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP3193346.1"
                     /translation="MNDRETRCYSGEVRAEQYDNAPTHILGYGSVFNSRSEPLWGFRE
                     IIKPGAFDDVLNDDVRSLFNHDPNFILGRSSAGTLSLSVDERGLRYDIVASDTPTICD
                     LVLSPMLRGDINQSSFAFRVARDGESWYEDDEGIVIREITRISRLYDVSPVTYPAYQD
                     ADSGVRSMKAWQEARASGALKKAVNERMARERLLTLLNA"
     gene            complement(5874..7100)
                     /locus_tag="IT904_003617"
     CDS             complement(5874..7100)
                     /locus_tag="IT904_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP3193347.1"
                     /translation="MLLDALFRSESLENPSVPVTGEAAETDNIFARDVYVSPETSMKL
                     AAVYACIYVISSSVAQMPLHVMRKTNEHVQPARDHPLFWLVHDEPNAWQTSYKWRELK
                     QRHVLGWGNGYTWVKRNRRGEVTSLECCMPWETTLLNTGGRHTYGVYNEEGAFAVSPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQQYTSAFFNGNARPAGIISVKNELNE
                     QSWGRLKNMWQRAVTALRSQENKTMLLPAQLDYRALTVSPVDAQIIDMTKLNRSMIAG
                     IFNVPAHMINDLEKATFSNITQQAIQFVRYTMMPWVANWEQELNRRLFTRTERAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVDGLDEMLVSVNAANP
                     LNNFKDTKGKEEKNDE"
     gene            complement(7248..9005)
                     /locus_tag="IT904_003618"
     CDS             complement(7248..9005)
                     /locus_tag="IT904_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP3193348.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPQHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVALFTAMSRFLVNG
                     GGVNDFLSTLDPDEDLLIL"
     gene            complement(9005..9487)
                     /locus_tag="IT904_003619"
     CDS             complement(9005..9487)
                     /locus_tag="IT904_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP3193349.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDSKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCEILDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDDVDPLAELLKARD
                     "
     gene            complement(9635..9985)
                     /locus_tag="IT904_003620"
     CDS             complement(9635..9985)
                     /locus_tag="IT904_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP3193350.1"
                     /translation="MPPRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLN"
BASE COUNT         2514 a         3027 c         2318 g         2558 t
ORIGIN      
        1 ccgaagttgc gcttcagcaa gagccacttc gtttctggct gcagtaatta atccggcagt
       61 tgcagatcca gcagaagacg ccatattgcc aaaatatcgg gctaccccga cggcaaccag
      121 agcaccggca gcggttgcca cggtgtcaat attgcctgca agaccattca gcaccccgga
      181 gagcgtcttc gtcactccgc ttgcctcgtt cgcaccacca acccaggcca taaaggcgtt
      241 ttcaactttg gttgcagagg atgaaaccgt atcaggcatt gctgcatatt catcacgcaa
      301 cgccccaagc tgactaatca gtgcaggaac aaccttatcg gcggttagtt ttccgttatc
      361 cgccatggcc ttcagatcct tacgggcaac gcccattccc gcagccagcg cacgaataac
      421 acgatcaccg ctctcattca ccgagttaaa ctcttcaccg cgcagcactc cctgcgccag
      481 tgcctgactg aactgcgtga tcaccgaact ggcttctgct gtactggcac cggataattt
      541 caggcccgtg gagatcgcct cggtgacttt cagtacctcc tcagaactgt aaccatactc
      601 ccgcatggaa gctgcagaac gggcaaaaag gctggcgtta tcagaaaacg ccgtccccgt
      661 tctctggctg atcgccatta attcacgttg tgatacctga aaatcatcac tggactgtga
      721 ggcctgcttc agacgggcat ttactgaatt ccactcatcg gcgagagaaa taagatgacc
      781 ggtagcaaaa gccgcggcaa atgcccccgc catattcagt gccgtagatt tagctgtatt
      841 tatctgatcc gtcacttctg ccagtgcacg ccgcatttca cgggatgcag cagcggactg
      901 ccggcctccg ttctgcatgg tacggtagta atcctgcccc atacgcgaag cccgggagat
      961 ctctgactgg aatgaccggg aatttgccga gattttaata atcagttcac gtaatgtcgc
     1021 cacactcatt ctccggacga aaaaaaaccg ccgaagcggt tatgttgact cactgagaca
     1081 ctattaaaag cgctttttcc agtccggcaa atggatctga tacgccttct gtctgctcct
     1141 tctcccactg aagaagcgca tcattcagtg gcactttgac cccctgcgca ccgtaaacag
     1201 ccgaaacaat ctgggcagcc cggatatcag cccgttcgtc ccccagcggg ctgaacctgt
     1261 caaattctgc ccacatcatg atttctgatg cggacatttc ccggcgtaac tctgacaatg
     1321 tgcgccccat cctgagcgcc agcatcatca gaaaacgcat ccccggaagc gctacttttt
     1381 tttaacctcg ccggcatcac tgatcagttc cagagactgc cgaagaagcc gcgcatgcac
     1441 cgggccatac acggcaatca cctgttcacg atcatcctct gaaaatacag gttgcagtcc
     1501 ggtatcacac agaacatcaa tgaacagttc aacatctgcc tccagatttc ggcgggcgcg
     1561 ctccgcaacg gataacggtg tctcattatc ttttgcttta acgatctcct gccagcgcaa
     1621 ccaggcttct gcagaaggtt cccgtaatac aaccgttgcc ccttcccatt caggcacatc
     1681 aacagtttta tggcgaaacc ccgacatcgt tgccagtgcc agattgcgga tatttttagt
     1741 catcacatct atcctcatta actgacggta acagtgcagg aagtggaggt caccttgtta
     1801 acagggcttg ctgaatcaga aatctcgcag gtatacgcac ccgcatcacc ggatgatgct
     1861 gatgccttac tgaacgttgc cgccgtctgt ccggaaacag gagaaccacc tttcttccag
     1921 acataagaat aaggcggcac accacccgca gcctcaaccg ccatttcaag tttcgctccg
     1981 gaagcaaccc gcagcgtgct ttttaaatca accttcactt tcagcgaagc tgtcgtcagc
     2041 acaggtttgc ctttcagacg cagggaaaac gttgcagcca caacaccatt ggttcctgca
     2101 gaccaggtat gctgacgcac ttctgccata aaggtaaatc cgttgcctga cggaaaaata
     2161 actttaaagc catacgtggt gtcattgtca taggcactgc gcaacgcgtt ctgggcagca
     2221 ttgaggtaaa agttgcctga catggaaatc tctgacgcgg caccaagacc gttaatattt
     2281 tcctgctcaa cagaacacag cgtggtgaca tcaatatcct gcttttgtcc agcggtaaac
     2341 tgcacttctt tgattgtaca gctcaggcca agatagctgg cagaatccag ggtttctgct
     2401 gttaccggtg cagacgaaat cataattttc gtcagttgcg aacgctcaaa attagaggac
     2461 atactcgtct cctgaaaata aaaaacccgc cagcggcggg tgggtaaaat cattaacgac
     2521 ctcaggctat tacctgaaat tcaagcgtgg ctctgctcag acgggaatca ggatcataac
     2581 cctgcgtttt agaaataacg gagggtgcca gttttcttac cgcatcaagc gcctgctcac
     2641 gaatattatc tgcgtcatca ggtactgtcg cccagacatc gatctgcacg gtaattctgg
     2701 attcagcctg cccatcaagc acatcagata ccgtgtcaga caccacagaa aacaccagcc
     2761 acggcggaga tactgcaggc tttccctccg tcagcgggac cacataagga taaacctgtc
     2821 ctccggccag ctgagacaac agggaataca gtgtggcctc cctcatttac ttaagacctc
     2881 atcaatagcc tgattcattc gctgtatggc aacctgcgcc gccagctcct ctgtcgtatc
     2941 gaaagccggg cgaatgaatg gatgcgcggg catgtttatc gttcccagct ccacaaagcg
     3001 ccagtaaaat gcatttcggg gatcgctggc tttcatgctg ttatcactgt ttccggttcg
     3061 caggttccgt ccgcgaatgt ggacacccga gataatttcc ccccgacgct ttgaacgctg
     3121 agtgagaaca accacatttt tcttcagttt cccggttcgc tccggcgcac gttcaacaac
     3181 tgcatcccgc ataacttcag caccggcacg ggtggcatcg cgcagaacct tattgttttc
     3241 tgccctgctg agcgtctcca aatcccgtgc aatatccgcc aggccggaaa aatcaagact
     3301 gaaatccatc acacattccc cttcaggctg cagagtattt caagccgggt agcgcgtgca
     3361 tccggtattg gtggaccttc tatacccaga atggcccctt taaatgcacc ggtcagcact
     3421 ttcagacgtg aagtcgctgt cacatcgcgc cggaatctca tccagactct gaccgtagac
     3481 tgagcggttt ctgctccgcc tgagattatc tccctcccgc tgataccctt aacttctgcc
     3541 catacggtag ctccctccgt caccgtctcc accggatgcc ctgacggaga gcgggcggtg
     3601 gtgacattca gaataattac gcgatcacgt aatctgcccg cctgcatgtc tcctcctaca
     3661 aaggaataaa acgataaggc tccagcagag aagaaaaacc aaacgggact ggtgccttgc
     3721 tgacatctga ggaattttcc cggttttcgt accagtgccc gaccagcaac atgagcgcca
     3781 gcaaaacatc atcagctata agcacccctt caggatcacc ttccggcacc gtctcctcat
     3841 aaagcttacg gttgataaaa ttttctgcct tgcggcaggc agcccggaaa tacagcatca
     3901 gtaactcatc atcagttgca tcatctgtat caatacggca ctgcgccctg agtttttcca
     3961 ctattgctgc catcagaaac tcctgcccgc aacactgtgc gggcataaaa aaaccgcgtc
     4021 ggcgcggtct gtaactgaac aacgagtggt tatttgccag tgagcgcctt gatggctgcc
     4081 acatcttcca gcacacagtc aaaacgatgg aaagccagaa atgccacctg atcaaactca
     4141 gcataacgct caaccagacg tttcagttcc atataagtaa cgcggcgaat gataaagcgg
     4201 ttgaaatccc ccaggaaaat gaattttttt ccagtaccaa tcccgtcaat agcctgatca
     4261 ataacataag ggatccccag cacagtagcc ggcgtaccgc ctgcaatatc cggcagccat
     4321 aacgggcgtt tctgtccatc ctccatctct tcaatagtct gcaatgtggc atcattgaat
     4381 gcccagcggt atttcggccc accacgatat gccggatcaa tggcatgttt cagggcattc
     4441 atttctttcc aggtgaaagc ggcagaggct gcagtctgga tggttcccgt caccgacgct
     4501 gccagccctt ttggctgtaa cggtgatccc gttccggtcc cctgaaccag atattttgcc
     4561 tctccacgac caatacgctg ggcaatacgg tttgccagat aagattcaat atccacccca
     4621 ctgtcctgga gcagctcatt ggacacacga attatttttg atgacagctt tttagccccc
     4681 agaatagcgg tcccgaacgt cacatcctgt tccgttgcgg ctgtattttc cgccagcagt
     4741 tcgccctctt cagtcgtgcc atcagacgtt gaccaggtga tatcctgccc ggttgatgtg
     4801 gtcagaagtt gcgccacact ggcaatcccg ccataagcct tcatggtgtc aatgattttg
     4861 ttacgcatct gcgtgggcac cgtatatccg ccctgagaat ccgttgttac actctgagcc
     4921 cgcagttcac gcatcagatt acgctcttca gcattcagtt ctgcaaatcc ggcacgcaga
     4981 aaacggttaa atgccgcagc acgcttctct tccaccgcct ttttcccgtt ctccgcctca
     5041 ttattctggc gctcttccgg cccggactca tccacatatg cctgatcctg acggcgcaac
     5101 tcttcttcac gggcgatttg ctcatccagc gcatccagct cagctttcgc cctgttccac
     5161 tctgcccgtt gctcatcagt ccatgcgtta tcaccaattt tttcatgcag tgcacgcata
     5221 tcctttgcaa tggtgtttcg tttttgcttc atctcatgaa gtttcatcgt cagtagtatc
     5281 cttatgcatt aagaagggtc aaaaggcgct cacgcgccat tcgttcatta acagctttct
     5341 tcagcgcacc actcgcccgc gcttcctgcc aggctttcat tgagcggaca ccggagtctg
     5401 cgtcctgata ggccggatat gtcaccgggc tgacgtcata cagacgagaa atgcgcgtga
     5461 tttcccggat aacaatcccc tcgtcgtctt cataccagct ctctccgtca cgggcgacac
     5521 gaaacgcgaa cgaggactgg ttaatgtcac cacgcaacat tggagacagc accaggtcac
     5581 aaatagtcgg agtatccgat gcaacaatgt cataacgtaa accgcgttca tccaccgaca
     5641 atgacaatgt gccggcagaa cttcgtccga gaatgaaatt aggatcatga ttaaacaagc
     5701 tacgtacatc atcattcagt acatcatcaa aagcccccgg cttgatgatt tcacgaaatc
     5761 cccacagagg ttctgaacga ctgttaaata ccgagccata ccccagaatg tgggtcgggg
     5821 cattatcata ttgttccgcc cgcacctccc cgctgtaaca gcgcgtttca cggtcattca
     5881 tcgttctttt cctctttgcc tttcgtatct ttaaagttat tcaacggatt tgctgcattt
     5941 acgctgacca gcatttcatc cagaccgtca accgggttca tatcctcaaa tgccctggct
     6001 tcattccgac tcatccagcc atctgtaatg gcaaagtgat agaactgcgc acgctcctgt
     6061 ggggtcccac ggagcaaccc cgtgaggtta aaacgaacgt aatacccggc agcccgttct
     6121 gtacgggtaa acaggcgacg gttaagctcc tgctcccagt tcgcaaccca gggcatcatc
     6181 gtgtagcgaa caaactgaat cgcctgctgc gtaatattcg aaaatgtggc tttttccagg
     6241 tcattaatca tgtgcgccgg gacattaaaa atccctgcaa tcatcgacct gttcagcttg
     6301 gtcatatcaa tgatctgagc atccaccgga gaaactgtca gagcgcggta atccagttgc
     6361 gcaggcaaca gcatggtttt attttcctgg ctgcgaagcg ctgtcaccgc ccgctgccac
     6421 atatttttaa gcctgcccca gctctgttcg ttcagttcat ttttcacaga aataataccg
     6481 gcaggacggg cattaccgtt aaaaaaggcg ctggtatact gctggccact cattcccata
     6541 ccaatggttt cagcatgctg catgatcggg ctcagtccca ttttctgatt gtttcccagc
     6601 gccctgatat ggatcatgtc gtccggactt accgcaaatg ccccctcttc gttatacacc
     6661 ccgtaagtat gacgcccacc ggtgttaagt aacgtggttt cccatggcat acagcattca
     6721 aggctggtaa cctctccacg acgattacgt tttacccacg tataaccatt gccccacccc
     6781 agcacatgac gctgcttcag ttcccgccac ttatagctgg tctgccaggc attaggttca
     6841 tcatgaacga gccagaacaa cggatgatcg cgtgcaggct gaacatgctc attcgttttt
     6901 cgcatcacat gcaggggcat ctgagccaca ctggatgaaa taacataaat acaggcatag
     6961 acagcagcca gcttcatgga tgtttccgga ctgacataca catcccgggc aaaaatatta
     7021 tccgtctcag cggcctctcc ggttaccgga accgagggat tttccagaga ctcactgcga
     7081 aacagagcat caagaagcat gttttctcct catggacact accagtgcat aaagcagcaa
     7141 caaacagccc gacagcatca gagacgctgg cagacctgca tacagataaa cgccagcagt
     7201 gagcaaaccg aaaccgatca gcccggtcat atcagtaata agctgtttca cagaattaac
     7261 aggtcctcat caggatcaag cgtggacaga aagtcattca cgcccccgcc atttaccaga
     7321 aagcggctca tggccgtaaa aagcgcaaca gggccgtcga ttttggcttc aggcgtggat
     7381 ttattcggga agatgttgtc gtttttgtcc ggttttaccg taacgttaga catcatccag
     7441 ttcatgaccg gatgattgct gtgatggaaa cgcccggcat aaaccagtga ttccgtttcc
     7501 ttcatggcct ctgacagatt gcggaccgtc tgcggaacct ccaccagcgg tatcccttct
     7561 tcagccagtg ccaggctgaa ctgcattgcg ctccacgggt caaatcccag ttccctgagg
     7621 ttttcaccgc caatccattc cagtaagtca ctttttatct gagcatgatc gataacatca
     7681 ccatccgtca ggatgagctt atccatctcc gcccacttcc ggtaaagttc tgcctgctgc
     7741 cgcgagcatc gctccagccg tccttccgga agccagaatt taaaatcagc atgaacatgt
     7801 ccgttatcgg ttcgccagag ttttgccgcc gcacagatat caatcttatg agcaaggtcg
     7861 acgccgaccc acatgggata tgttttcagc tcatgttgtg gagcaatgta ttcgcacttc
     7921 tcccacttaa tcatatccat ccaggcagat tcggcagtga cccacacatt catgtgtttg
     7981 gtaaaaaaat tcacccgcgc agagacctgc tccttcgctt ttttcgccag acgacgcaga
     8041 tcatcccagc gtttacagat gcccaggcca ggattcgctt tctgccagac cgtttcatca
     8101 aacggatcat ctccctcatc gagcgtgtaa atgatcgcaa agtaggagtc gtcttttacc
     8161 gcgccctcca cgtcgctgtt atagcctcgc aataccttaa tggcgtaatc gcgctgctcg
     8221 tagcaaatcc cttccttgtt aaagccagcc gtggtgatgc caaataacag agactgcaga
     8281 cgggcaccgg ttgccgtttc cagaacgtcc cacacgtcac gggttttatg agcatgcagc
     8341 tcatcaataa tggcgcagtg gatgttcaga ccgtccaggt tgtttgcatc cgaggaaagc
     8401 ggttcaaatt ttgatgcgct ctgctcctgg taaatcgcca gcttgttgaa atcaaacaac
     8461 cgcccgagtg tcgaccgggc ttttctgacc atatttttgg cgtcttcaaa cacaattctg
     8521 gcctggtcac gcgtggttgc ggctgaatac acctcagccc cgccttcacc atctgccccc
     8581 gtcatataca gaccgatacc cgatgacagg gttgattttg cgtttttacg ggcaacttcg
     8641 ttgtatgctg tccggaaccg gcgcaccatc accgggcgtc cactgccatc gctgcgcatg
     8701 acaacttccc cggtttcttc attcaccagc ggaatgacaa aaccaaaaat attaatgagg
     8761 ataaacacat gccagtccat caactcaatg ggctgacctg ccagcgcccc tttcacatgg
     8821 ggtacaaatt tgtagaaatt aaggatgtgc tgtgcacggg gttcactgaa ataaatcccc
     8881 cgctcttcgc cgtacttcag atcatcaaga aaacgctggc aggccagacg gacaaattcg
     8941 ccagcaacaa tttctcctgc aacaacacgt tcggcgtagc ggatcccgtc agccactttt
     9001 gccatcagtc tctcgctttt aaaagctctg ccagcggatc aacatcatcc ggtccggcgg
     9061 tatttacttt cgcccggctt gccggtgaca taccaaactc tgcaagcatc gcccgaatcc
     9121 gcttccaggc atcagccttc atcgcagcag ccgggtgtgc cttgatcagc acatcgccat
     9181 tctgcgtttc cgtgcggtag gtataaccct caacatcgag tatttcgcag tgatgccggt
     9241 attcggtgta ggcttccacc agtaactcga gcgcacgtgc atcgagctga gaaatgatcc
     9301 cttccgcatt cagctcttcc gccattcgcc tgaaccagta cttcccctgc gcccctaaat
     9361 gttgcggaat tttagggaga cctttttcat cctttttagc ggtttttttg gagtctttaa
     9421 ctggccgctt tgaggggtta cctcgtatca aatgcaggcg tggcggggtt ttcgggggtc
     9481 cagacataat cggttttacc tatcaatcat ttaatcacat tccaaaaaaa agttttcgaa
     9541 cctgcggcga tgcgaggaaa ggtcaggcgg cggtactgag cagccagggt tgcagagatt
     9601 tgacctgccc ctcccctaca gatgggaact gttatcaatt gaggcgttcg cgcgctgttt
     9661 ttgctttatg acagggccag cacagactct gcaggttact gtctgcatcc gtgccaccat
     9721 gagctttcgg aatgatgtgg tccacagttc tggcttcaac ggctctccca ttgcgcaggc
     9781 agttctgaca cagatgatta tcacgcttca gtatgcgcgc acgtatggca tcccatttcg
     9841 agccatagcc acgctggtgg cggctcagtc cgcgttgatg ctgtacccat ccttcgccac
     9901 gatgtttatc gcagtaacca gaactgtctg tggttgtacc tgcacatcca cgcttacggc
     9961 aggcgcgtgg gattcgtggg ggcatatgta ctccaatgaa gaagccaccg acatagcctc
    10021 ctccattcat agtgaaacta ttttcatcta cccagtaatg aattctttgt agagttgtga
    10081 tcaatacaac tcactaatgg agaggcttgt ccaacacgtt ggacaagttt cctgtttgat
    10141 ttactggaca ctatagaagg acagaatgcc ttcatcactc gaataacatc aattaaggag
    10201 gttcaacatg tttcattcca caaatcatca ggctgtaatt atggctgcat cagcttgtgc
    10261 cacagacctt ttccgcttca ctttgagcct gattcatttc tacctgaccg gctcgcctct
    10321 atctttttaa tccccgcttt atccaaattg cattgccaga atgccgacaa cagactgaca
    10381 ttcaaatcct gactacctcc aataggctga gcatcca
//
LOCUS       DACAPB010000158        10349 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.158, whole genome shotgun sequence.
ACCESSION   DACAPB010000158 DACAPB010000000
VERSION     DACAPB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.158"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..349
                     /locus_tag="IT904_003621"
     CDS             <1..349
                     /locus_tag="IT904_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAP3193351.1"
                     /translation="LQIQLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLG
                     LSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLT
                     HWLDYPHLPYSPE"
     gene            502..960
                     /locus_tag="IT904_003622"
     CDS             502..960
                     /locus_tag="IT904_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP3193352.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            990..2762
                     /locus_tag="IT904_003623"
     CDS             990..2762
                     /locus_tag="IT904_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP3193353.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            2755..4536
                     /locus_tag="IT904_003624"
     CDS             2755..4536
                     /locus_tag="IT904_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP3193354.1"
                     /translation="MRSFSQLLPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4717..5055
                     /gene="glnK"
                     /locus_tag="IT904_003625"
     CDS             4717..5055
                     /gene="glnK"
                     /locus_tag="IT904_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP3193355.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            5085..6371
                     /gene="amtB"
                     /locus_tag="IT904_003626"
     CDS             5085..6371
                     /gene="amtB"
                     /locus_tag="IT904_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAP3193356.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(6420..7280)
                     /gene="tesB"
                     /locus_tag="IT904_003627"
     CDS             complement(6420..7280)
                     /gene="tesB"
                     /locus_tag="IT904_003627"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAP3193357.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            7498..8070
                     /locus_tag="IT904_003628"
     CDS             7498..8070
                     /locus_tag="IT904_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193358.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(8101..8412)
                     /locus_tag="IT904_003629"
     CDS             complement(8101..8412)
                     /locus_tag="IT904_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAP3193359.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            8575..8671
                     /gene="ffs"
                     /locus_tag="IT904_003630"
     ncRNA           8575..8671
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IT904_003630"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8812..9144
                     /locus_tag="IT904_003631"
     CDS             8812..9144
                     /locus_tag="IT904_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HAP3193360.1"
                     /translation="MVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWASDVPDGKV
                     TDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFDGKRMIYG
                     GFESIIDE"
     gene            complement(9186..>10349)
                     /gene="pdeB"
                     /locus_tag="IT904_003632"
     CDS             complement(9186..>10349)
                     /gene="pdeB"
                     /locus_tag="IT904_003632"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAP3193361.1"
                     /translation="VWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQID
                     AAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPLPEMNISIITWAS
                     TKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQ
                     PIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMG
                     DWLRQHPQQHISINLESPVLTSEKIPQLLRDMINHYQVNPRQIALELTEREFADPKTS
                     APIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIE
                     MAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT         2314 a         2554 c         2916 g         2565 t
ORIGIN      
        1 cttgcagatc cagctatacg aagcattagg cgagcgtgca catttgtgtt tttccgccac
       61 ggattgcctc gaagtgctgc cggtgggctg caataaaggc gctgcattga cggtgctgac
      121 ccaacattta ggtttatcgt tgcgcgattg catggccttt ggtgatgcga tgaacgatcg
      181 cgaaatgtta ggcagcgtcg gtagcggatt tattatgggc aatgcgatgc cgcaactgcg
      241 cgcggagctc ccgcatttac cggtgattgg acattgccga aatcaggctg tctctcacta
      301 tttgacgcac tggctggact atccacatct accttattcc cccgaataac gagatccctt
      361 ccagcaccgg gcaattgccc ggtttttttt gcgttgaatt tgtcattttg tgccgtggtg
      421 tttaaaccgc acagaataaa ttgtcgtgat ttcaccttta aaatagaatt aaaagagaaa
      481 aaaattctct gtggaagggc tatgttagat aaaattgacc gtaagctgct ggccttactg
      541 cagcaggatt gcaccctctc tttgcaggca ctggctgaag ccgttaatct gacaaccacc
      601 ccttgctgga agcgcctgaa acggctggag gacgacggta tccttatcgg caaagtcgcc
      661 ctgctggatc cggaaaaaat aggcctcggc ctgaccgctt ttgtgctgat aaaaacgcaa
      721 catcacagca gcgaatggta ttgccgcttt gtcacggtgg ttaccgaaat gccagaagtg
      781 ctggggttct ggcgcatggc tggtgaatac gattatctga tgcgcgtcca ggttgccgac
      841 atgaaacgct acgacgagtt ttataagcgt ctggtaaaca gcgtgccggg gctgtcggac
      901 gtcacttcca gcttcgcgat ggaacagatt aaatacacca cttctttacc catcgaataa
      961 atatccagaa tcaggtcagg acacaacgcg tgcgattatt tgctcaatta agctggtatt
     1021 tccgtcggga atggcgtcgc tatctcgggg ctgtcgcctt gcttgtcatt atcgcgatgc
     1081 tgcaactggt tccaccaaaa gtggttggta ttgttgtcga tggcgtgaca gaacaacact
     1141 ttactaccgg gcagatcctg atgtggatcg ccaccatggt gctgattgcc gttgtggttt
     1201 atctcctgcg ttacgtctgg cgcgtattgc tgtttggtgc gtcttatcaa ctggctgttg
     1261 aactgcgtga agattattac cgtcagctaa gccggcagca tcctgagttt tacctgcgtc
     1321 atcgcacggg tgacctcatg gctcgtgcga ctaatgacgt cgatcgcgtc gtgtttgccg
     1381 ccggagaagg ggtgctgacg ctggtggatt cactggtaat gggctgcgct gtgttgatta
     1441 tgatgtctac gcaaattagc tggcagttga ccttatttgc cctgttaccg atgccagtga
     1501 tggcgatcat gattaagcgc aacggcgatg ctttgcatga acgctttaag ctggcacagg
     1561 cggcgttttc cagtcttaat gaccgcaccc aggaaagcct caccagtatc cgcatgatca
     1621 aagcctttgg tctggaagat cgccagtcgg cgttatttgc cgcggatgcc gaagataccg
     1681 gcaaaaaaaa catgcgggtg gcgcgtattg atgctcgttt cgacccgacc atctatatcg
     1741 cgattggtat ggcgaacttg ctggcgattg gcggcggtag ttggatggtg gtgcagggca
     1801 gtttaacgct gggccagctc accagtttta tgatgtattt aggtctgatg atttggccaa
     1861 tgctggcgct ggcatggatg tttaacattg tggaacgtgg tagtgctgcg tacagccgta
     1921 ttcgcgcgat gctggcggaa gcgccggtgg tgaacgatgg tagcgaaccc gtgccggaag
     1981 ggcgtggcga actggatgta aatattcacc agttcacgta tccgcagact gaccatcctg
     2041 cgctggaaaa cgttaatttc gccctgaaac ccggtcagat gctgggtatc tgcgggccga
     2101 ctggttccgg caaaagtacc ctgttgtcgc tcattcagcg tcatttcgac gtcagcgagg
     2161 gggatattcg ctttcatgat attcctctga cgaagttaca actcgatagc tggcgtagcc
     2221 gcctggcggt agttagccag acgccattcc ttttttctga cactgtggcg aataacatcg
     2281 cgctgggttg cccgaatgcc acccagcaag agattgagca tgtcgcgcgg ttagccagcg
     2341 tacatgacga tattttgcgt ctaccgcaag gttacgatac agaggtgggc gagcgcggtg
     2401 tgatgctttc cggcgggcaa aaacagcgta tctccattgc tcgtgcgtta ttagtcaacg
     2461 cggaaatcct catccttgat gatgcgcttt cggcggtgga cggacgcact gagcaccaga
     2521 tcctgcataa cctgcgtcag tgggggcagg gaagaacggt aatcatcagt gcccatcgcc
     2581 tttctgcact gacggaagcc agtgaaatta ttgtgatgca gcacggacat atcgcccagc
     2641 gtggcaatca tgatgtgctg gcacaacaaa gcggctggta tcgcgatatg tatcgctatc
     2701 aacaactgga ggcggcgctc gacgacgctc cggaaaatcg cgaggaggcc gtcgatgcgt
     2761 agttttagcc aactgttgcc gactctcaag cgcctgttag cgtacggttc accgtggcgt
     2821 aaaccgctgg ggattgcggt cctgatgatg tgggttgcgg cggcggcaga agtcagtggg
     2881 ccgctgctta tcagctattt tatcgacaat atggtagcga aaaataacct gccgttgaaa
     2941 gtggttgcag ggctggctgc ggcgtatgtt gggctgcaac tgtttgccgc cgggctacat
     3001 tacgcgcagt cgctgctgtt taatcgggcg gcagtaggcg tagtgcaaca gttgcgtacc
     3061 gacgtgatgg atgctgcgtt acgccagcca ttaagcgagt ttgataccca acccgtcggg
     3121 caggtgattt cccgcgtcac taatgacact gaagtgatcc gcgatctcta cgttaccgta
     3181 gtggcaactg tcctgcgcag tgccgcgctg gtgggcgcga tgctggtggc gatgttcagc
     3241 ctcgactggc gaatggcact ggtggcgata atgattttcc cggtggtgct ggtggtaatg
     3301 gtgatatacc agcgttacag cacgccgatt gtccgtcgtg tgcgcgccta tctggcggat
     3361 atcaacgacg gctttaacga aatcatcaat ggcatgagcg ttatccagca gtttcgtcag
     3421 caggcgcgat ttggcgaacg tatgggggag gccagtcgtt cacactatat ggcgaggatg
     3481 caaaccctgc gcctcgacgg ttttctgctg cgtccgctgc tgagtctgtt ttcatcgctc
     3541 attctttgtg gcttgttgat gctgtttggc ttctccgcca gcggcaccat tgaagtgggc
     3601 gtgctgtatg cgtttatcag ctatcttggg cgacttaacg aaccattaat cgaactgacc
     3661 acgcaacagg cgatgctgca acaggctgtt gttgctggtg agcgcgtgtt tgaactgatg
     3721 gacggaccgc gccagcaata tggcaatgat gatcgcccgt tacagagtgg caccatcgaa
     3781 gtcgataacg tgtcatttgc ttatcgcgat gacaatctgg tgctaaagaa cattaatctc
     3841 tctgtgcctt cgcgcaattt tgtggcgctg gtcgggcata ccggcagtgg caaaagcacc
     3901 ctcgccagtt tattgatggg ctattacccg ctaacggaag gtgagattcg ccttgatggt
     3961 cgtccattaa gttcgctaag tcacagcgcg ctgcgccagg gcgtggcaat ggtgcagcaa
     4021 gatccggtgg tgctggcgga taccttcctc gccaacgtga cgctggggcg ggatatctcc
     4081 gaagaacgcg tctggcaggc gctggaaacc gtgcaactgg cggagctggc gcgtagcatg
     4141 agcgacggta tttacacgcc gctgggcgag caggggaata atctctcagt tgggcaaaag
     4201 caactgctgg cactggcgcg cgtgctggtc gagacgccgc aaatcctgat ccttgatgag
     4261 gcaaccgcca gcattgactc cggtactgaa caggcgattc aacatgctct ggcggcggtg
     4321 cgtgaacata ccacgctggt agtgattgct caccgcttat cgaccattgt tgatgccgac
     4381 accattctgg tgcttcatcg tgggcaagcc gtggagcagg gcactcacca gcaactgctg
     4441 gcggcccagg gacgctactg gcagatgtat caactgcaac ttgcgggcga agagctggca
     4501 gccagcgtgc gtgaagagga atcattgagc gcctgaatag cgcaatattt catcgttggt
     4561 gcaaaaatgt aacgcactgt gcactgtcat agtgcgtttt cattttcaaa cttcttaact
     4621 tcctgctctc tttctcgttt ttcatttctg gcacaccgct tgcaatacct tcttcgtgta
     4681 gcagaaccat taccgaattc tgaccggagg ggatctatga agctggtgac cgtgataatc
     4741 aaaccattca agctggaaga cgttcgtgaa gcgttatctt ccattggtat tcagggcctg
     4801 accgtcaccg aagtgaaagg tttcgggcgt cagaaagggc atgccgagct gtaccggggg
     4861 gcggaataca gcgtcaattt cctgccaaaa gtaaaaattg atgtggcgat tgctgatgac
     4921 caactcgatg aagtgatcga tatcgtcagt aaggcggctt acaccggaaa aattggcgac
     4981 ggcaaaatct tcgtcgctga attgcaacgc gtcattcgta ttcgtaccgg cgaagccgac
     5041 gaagcggcgc tgtaatctct ggcacacagc aacaggaacg aaaaatgaag atagcgacga
     5101 taaaaactgg gcttgcttca ctggcgatgc ttccgggact ggtaatggct gcacctgcgg
     5161 tggccgataa agccgacaat gcgtttatga tgatttgtac tgcgctggtg ctgtttatga
     5221 ctattccggg gattgccctg ttttacggtg ggttgattcg cggcaaaaac gtgctgtcga
     5281 tgctgacgca ggtgacggtg acatttgcac tggtctgtat tctctgggtg gtttacggtt
     5341 actcgctggc gtttggtgag ggcaacaact tcttcggcaa cattaactgg ttgatgctga
     5401 aaaacatcga actgacggcg gtgatgggca gcatttatca gtatatccac gtggcgtttc
     5461 agggatcgtt tgcctgcatt accgtcggct tgatagttgg ggcgctggcg gaacgaatcc
     5521 gcttctcagc agtgttgatt ttcgtggtgg tatggctgac gctctcttac attccgattg
     5581 cgcatatggt gtggggcggt ggtttgctgg cttctcacgg tgcgctggat ttcgcgggtg
     5641 gcaccgtggt gcacattaac gccgcaatcg ccggtctggt gggcgcgtat ctgataggaa
     5701 aacgcgtggg cttcggtaaa gaggcgttta aaccgcacaa cctgccgatg gtcttcaccg
     5761 ggactgccat tctctatatc ggttggtttg gctttaacgc cgggtcagcg ggcacggcga
     5821 atgaaatcgc ggcactggca tttgtgaata ctgtggtcgc aacggcggcg gcaattcttg
     5881 gctggatctt cggtgaatgg gcgctgcgtg gtaagccttc actgctgggg gcgtgttctg
     5941 gcgcgattgc cggtctggtc ggcgtgacgc cagcctgcgg ctacattggg gttggcggcg
     6001 cgttgattat cggcgtggta gctggtctgg cgggcttgtg gggcgttacc atgctcaaac
     6061 gcttgctgcg ggtggatgat ccctgcgatg tcttcggtgt gcacggcgtt tgtggcattg
     6121 tcggctgtat catgaccggg atttttgccg ccagctcgct gggcggcgtg ggcttcgctg
     6181 aaggtgtgac gatgggccat cagttgctgg tacagctgga aagcatcgcc attacgatcg
     6241 tctggtccgg tgttgtggca tttatcggct acaaattggc ggatctgacg gttggtctgc
     6301 gtgtaccgga agagcaggag cgagaagggc tggatgtcaa cagccacggc gagaatgcct
     6361 ataacgcgta acaagcactg caaaaaacag ccggacggtt ttcacctccg gctatttttt
     6421 taattgtgat tacgcatcac cccttcctga acggtcgagg caaccagtac gccgtcttgg
     6481 gtataaaact caccgcgcac aaagccacgt gcgctggacg ccgaggtgct ctccacgcta
     6541 tacagcagcc attcattcaa attaaacggg cgatggaacc acatggaatg gtcaatggtg
     6601 gcaatctgaa tccccggttc gagaaaaccg atgccgtgcg gctgtagagc taccggcagg
     6661 aagttaagat cagaagcgta accgagcaga tactgatgaa cgcgcaggtc atccggcacg
     6721 ctaccatttg cgcggatcca cacctgacga tgtggttctg cgacgtgacc tttcagtggg
     6781 ttatgaaact ccaccggacg gacttccagc ggacgatcgc agatgaattt atctttcagc
     6841 actggcggca gcaggtgcgc cagcgattgg gcgatttgcg tttccgaagg taggccatca
     6901 ggcgctggcg cggacggcat tgttttttga tgttcgaaac ccgcttctgg tgcctggaaa
     6961 gaggcagtca tataaaaaat cggtttgccg ttttgaatag cagcaacccg gcgggcgctg
     7021 aagctgttac cgtcacgcag cgtttcgaca tcataaataa tcggcttctt actatcgcca
     7081 gggcgaagaa agtagctgtg aaacgaatgt accagccgct cttcagggac ggtctctttt
     7141 gcagcataca aggcctgacc cacgacctgg ccgccaaaca cctggcgtaa acctaaatct
     7201 tcactctggc cgcgaaagag tccttcctca attttttcca gatttaacaa tgtcagtaaa
     7261 ttttttagcg cctgactcat ataactctcc agtaacaaag ctgccgcagc aagccaaagt
     7321 gagttgagta taacgcaaat ttgctactgg tccgatgggt gcaatggtct gaattacggg
     7381 ctaattacag gcagaaatgc gtgatgtgtg ccacacttgt tgacgttact attttgttaa
     7441 ccactcttcc ggcgaggaaa gttagcccgc tggtgcattg ataataagga gaaatgaatg
     7501 aaactcgtgc acatggccag tggtttagcg gttgcgattg cgttggcggc ttgcgcagat
     7561 aaaagcgcgg atattcagac gccagcaccg gctgcaaata cgtctatttc agcaacacaa
     7621 caaccagcta tccagcaacc gaatgtctcc ggtaccgtct ggatccgtca gaaagtcgca
     7681 ctgccgcctg atgctgtgct gaccgtgaca ctttctgacg cgtcgttagc cgatgcaccg
     7741 tcaaaagtgc tggcgcagaa agcggtgcgt actgaaggta aacagtcacc attcagcttt
     7801 gttctgccat ttaacccggc agatgttcag ccgaacgcgc gtattctgtt gagtgcggcg
     7861 attaccgtga atgacaaact ggtatttatc accgataccg ttcagccggt gatcaaccag
     7921 ggcggaacta aagccgacct gacattggtg ccggtacagc aaaccgccgt gccggttcag
     7981 gccagcggtg gcgcaacgac taccgtacct tcgacttcac caactcaggt gaatccgtct
     8041 tcggcagttc cagctcctac gcaatattaa gcgacgttaa ccctctccgc ccggagaggg
     8101 ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata ccatcacacc
     8161 ttctgccagt aatgcctgtc gctgacgctg taaatccggt ccggttagcg aaattgtgcc
     8221 gtggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac gctttaacac
     8281 accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat caccgtaagt
     8341 ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg gaaatgaatc
     8401 ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa aaacgcccga
     8461 gtgaagtcgc attgcgcaag aaaccagcat ctggcacacg atgggttgca attagccggg
     8521 gcagcagtga taatgcgcct gcgcgttggt tctcaacgct ctcaatgggg gctctgttgg
     8581 ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag ccaaggcaga
     8641 tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct cccaccgttt
     8701 cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa gaattgtctg
     8761 gctgcgcagt acgcttcgga ggtatgtctg atgaagtagg ttgatggttt tgtggttgcc
     8821 gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggctgc gccattgttt
     8881 aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc ggatggcaaa
     8941 gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt ctttagctgg
     9001 attgaatacc cttcaaaaga ggtccgcgac gctgctaatc aaaagatgat gtcggaccca
     9061 cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat ctatggcgga
     9121 ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc cgtcagccca
     9181 aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa tgccttgctg
     9241 tagagccagc cctgaccgta gtgcacgcca tgctggcgta accactcttc ttgtttactg
     9301 gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc gatgatatgc
     9361 ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt cagaatgtcg
     9421 acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc atcaagataa
     9481 atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt cggatcggca
     9541 aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata gtgattgatc
     9601 atgtcacgca gcaattgcgg gattttttcc gaggtgagca cgggggattc aagattgatc
     9661 gaaatatgct gctgtggatg ctgacgcagc cagtcgccca tatcttcaaa gacgcttctt
     9721 ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg aataaaacta
     9781 tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc agcaccgaca
     9841 attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc acgattttcg
     9901 atagcatcct gcagccgatg atgcggtgac tgaatacggc gcaaaatacg cagcacaaac
     9961 atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac ttgccgatgc
    10021 cagcctttct gcaacatttt cgttgaagcc catgtgataa tcgaaatatt catctccggt
    10081 aagggcagga tatcgtaaat gattccatta ttttcgatat gctcaccggg tgttttttgt
    10141 agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac gttatgggca
    10201 ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc aataaaggaa
    10261 gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta acggataatg
    10321 cctaaatcgt tatgcgatgt taaccagac
//
LOCUS       DACAPB010000159        10342 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.159, whole genome shotgun sequence.
ACCESSION   DACAPB010000159 DACAPB010000000
VERSION     DACAPB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.159"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..234
                     /locus_tag="IT904_003633"
     CDS             <1..234
                     /locus_tag="IT904_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="camphor resistance protein CrcB; may be involved in
                     chromosome condensation; overexpression in Escherichia
                     coli protects against decondensation by camphor;
                     overexpressing the protein results in an increase in
                     supercoiling; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAP3193362.1"
                     /translation="TALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQA
                     GNYIWALTSVLVHVIGSLIMTALGFFIITILFA"
     gene            complement(614..1807)
                     /locus_tag="IT904_003634"
     CDS             complement(614..1807)
                     /locus_tag="IT904_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3193363.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            1943..3667
                     /gene="iucA"
                     /locus_tag="IT904_003635"
     CDS             1943..3667
                     /gene="iucA"
                     /locus_tag="IT904_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAP3193364.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3668..4615
                     /gene="iucB"
                     /locus_tag="IT904_003636"
     CDS             3668..4615
                     /gene="iucB"
                     /locus_tag="IT904_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAP3193365.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGKMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4615..6357
                     /gene="iucC"
                     /locus_tag="IT904_003637"
     CDS             4615..6357
                     /gene="iucC"
                     /locus_tag="IT904_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAP3193366.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6354..7631
                     /gene="iucD"
                     /locus_tag="IT904_003638"
     CDS             6354..7631
                     /gene="iucD"
                     /locus_tag="IT904_003638"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAP3193367.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            7713..9914
                     /gene="iutA"
                     /locus_tag="IT904_003639"
     CDS             7713..9914
                     /gene="iutA"
                     /locus_tag="IT904_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAP3193368.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAVFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2485 a         2702 c         2686 g         2469 t
ORIGIN      
        1 actgcgttgg cgtacttttt acgacaacca caccttgatc ctttttggaa attaatgata
       61 acaacgggac tttgtggagg tctgagtact ttctctacat tttctgttga agtattcgca
      121 ttactccagg cgggaaatta tatatgggct ttgacgtctg tacttgttca cgtcattggc
      181 tccctcatca tgacagcttt aggctttttt atcataacca tacttttcgc atgagaattc
      241 attaacgtta caggtttatc aactaatgtg acgcttagga tagaatttcg ctccatggta
      301 tgcgtttgca gtcattacag tctccatgac gactgtaatg actgcaaacg cattacctga
      361 catatcagta taaacaatga attaacctct tggcgtattt tttacatcac tggccctcac
      421 cccccaaaat tctctctgaa aaattgttgc ggttaacgtt gccaatgtat ccacccattt
      481 cagaagacga tctggcatcc tggtggcatg ctgcgatgtg cttcaggtga gtgcactggg
      541 cggtcagcac aggcggtttc gacagcgaaa ttgcacagga cgttatgccg gctgtgagta
      601 caacatcata gccttacttg attgtacggt aatgccaaat acatgcagac actatggtaa
      661 ataatacagc ggcaaataat gccgtcacaa tcagcgcgat tgagacacct gttttcgacg
      721 ccagtggcac caacaatgcg cacagcccat agccaagtgt gtgactggcc gcaagccagc
      781 ccgcgcctgc gccgtcggcc agcttatcat tcagtaaaag ttgataagcg ggtgttgcca
      841 gagcggcccc aaatgacagc actgcacagc ccaggtaaaa caaccatagc tgttcagtga
      901 gcatgatagc cagaccacta ctcatcaaca ccccggcact caggagcaga gccactggag
      961 tcaggcgctg tggacggagt accacgaact gcgcgataag cgcagctatt gcggacagcc
     1021 ccaacaacca cgccacctgt tggctaatag tggtggtatc agtatcgaac tggcgagtaa
     1081 gggctggcga aagtccaagc tgcatcatgc tcattgccgc agccagtaac attgcgcaaa
     1141 gcagataagg caggcaatcc cgcttcaggc tgacgctctt gcgctccggt gtgggctgtg
     1201 gtggtgtgcc gggcaaccgc agaagcatca ccacagccca caccggagct gccatcagca
     1261 tccacactgg cgccagaggg tgaatgacca acattgccgc cgcgcacagc ggcccgaata
     1321 gcctgccgca gctcaggccg gagctgatgg ttgccagagc ggccatgcga ttcccttctc
     1381 ccgctctctg caatgcccag acctgacagg ctggcaccat tgctgacacg gtcagaccgt
     1441 agacgatccg ggcgatgatt aatattccca accccaccgc tgttgttacg gcaccggtag
     1501 ccatcagcac gcttcccagc ccaagcaggg taaagcttac gccatatcct cccagagccc
     1561 acagcaccac agtcttactg ccagtaaggg aaatttgctt tccccaccat ggggaagatg
     1621 gcagaaacag cattgagccc agcatcagca gggcagccca tacagacaga ctcagatttg
     1681 tctgtatgac cagaacaggg agtacaacca gcagaccgtt ctgtccgata ccgagaagac
     1741 cggcactgaa cgccagtggc cagcaggaca gggttttttg gggcgtatct tcgatcttag
     1801 atgacattgt aatgtttcaa caccatgtat taattgtgtt tatttgtaaa attaattttt
     1861 ctgacaataa catttctcat tgataatgag aatcattatt gacataattg ttattatttt
     1921 actgtgtagg agctgtttga ttatgatcct gccctctgaa aaatccgcca cagatgtggc
     1981 tgcgcagtgc ttcctgaatg ccttgattcg tgaaaccaaa gactggcaac tggcggaata
     2041 cccgccagac gaattgatta tcccgctgga cgaacagaag tcgctccatt tcagagtggc
     2101 ttatttctcc ccaacccagc atcaccgctt tgcatttcct gctcatctgg tcacggcatc
     2161 aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata agctccggca
     2221 tcaacttttt ctgccagttc ccctctgcga aactttccac caacgcgtgc tggaaagcta
     2281 cgcccatacg caacagacaa ttgatgcccg ccatgactgg gccatcctgc gtgaaaaagc
     2341 gttgaatttt ggcgaggctg agcaggcact gctgacagga cacgctttcc accctgcacc
     2401 taagtctcat gaaccgttta accgccagga agctgaacga tacctgcctg acatggcacc
     2461 tcacttcccg ctgcggtggt tttcggtgga taaaacgcaa atcgctggtg aaagtctgca
     2521 tcttaacctt caacagcggt tgacgcgatt tgccgcagaa aatgcgcccc agttactcaa
     2581 cgaattaagt gacaatcaat ggctgttccc gctgcacccg tggcagggag aatatctttt
     2641 gcagcaagtg tggtgccagg cactttttgc taaaggactt atcagagact taggcgaggc
     2701 cggcacgtcg tggctgccga ccacctcttc ccgctccctc tactgtgcta ccagccgcga
     2761 tatgatcaag ttctccctga gcgttcggct gaccaactcc gtccgtactc tgtctgtgaa
     2821 agaggtggag cgaggaatgc gcctggcacg tctggcgcaa accgacggct ggcagatgct
     2881 acaggcccgc ttccctactt tccgggtaat gcaggaggac ggctgggccg ggctacgcga
     2941 tcttaacggc aacatcatgc aggaaagttt gttttccctg cgtgaaaacc tgctgctgga
     3001 gcagccgcaa agccagacca acgttctggt ttccctgact caggccgcac cggatggcgg
     3061 tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta tcactgtgca
     3121 acaggccgcc cacgcatggg tcgatgctta ctgtcagcag gtgctgaagc ctctgtttac
     3181 ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc ttgtgcagat
     3241 gcttggggat ctgccggtcg gatttattta ccgtgactgc cagggcagcg cgtttatgcc
     3301 tcacgcgaca gagtggctcg ataccattga cgaggcgcag gcggaaaata ttttcacccg
     3361 tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt ttgccgtaac
     3421 tgctgcgctg ggtgctgccg ggctggacag cgaagcgaat ctgatggctc gtgtacgaac
     3481 attgctggct gaagtgcgtg accaggtgac tcataaaacc tgtctcaact atgttctgga
     3541 aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc ataacgagaa
     3601 caccatcgtt gacccttcgg tgatctattt tgatttcgct aacccgctgc aggctcagga
     3661 ggtctgaatg tctggggcaa acattgttca cagcggatat ggactgcgct gtgaaaaact
     3721 cgacaagcct ctgaatcttg gctgggggct ggacaatagc gcggtgttgc actggcccgg
     3781 ggagctgcca acagggtggc tgtgcgacgc gctggatcag atatttatcg ccgcaccaca
     3841 actttcagca gtggttcttc cctggtccga atggtgtgag gagccacagg cgctgacgct
     3901 tttcggacag gtacaaagcg acattatcca tcgttccgct ttctggcagt taccgttatg
     3961 gctgagttct ccggcaaacc gggcctccgg taaaatggtt tttgatgcag agcgtgagat
     4021 ttatttcccg cagcgccccc cccgtccgca gggtgaagtt tatcgtcgtt acgatccacg
     4081 cattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag aacgtttcac
     4141 tcgctggatg aacgatccgc gcgttgagta tttctgggag caaagtggct cactggaggt
     4201 acagatcgcc tatctggaac gccagctcac cagtaaacat gcattcccgc tgattggttg
     4261 tttcgacgat cggccgttca gttatttcga aatctactgg gcggcggaag accgcattgg
     4321 tcgccactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg gtgaacagca
     4381 atggcgcgga gcccactatg tgcaaagctg gctgcgcggg gtgacacatt acctgctgct
     4441 gaatgagcca cgtacgcagc gcacagtact ggagccacgc accgataacc agcgcctgtt
     4501 ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc cacataaacg
     4561 ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat
     4621 cacaaagact gggatttggt caaccgccgc ctggtggcaa aaatgttgtc tgagctggag
     4681 tatgagcagg ttttccacgc ggaatctcag ggcgatgacc gctactgcat taacctgccg
     4741 ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctctg gattgatgct
     4801 caaactctgc gctgcgcgga cgagccagta ctggctcaga cgctgctgat gcagctaaag
     4861 caggtactgt caatgagcga tgcaaccgtt gctgagcata tgcaggattt gtatgccacg
     4921 ctgctgggcg acctgcaact actgaaagcc cgtcgcgggc tgagcgccag tgacctgatt
     4981 aatcttaatg ccgaccgcct gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat
     5041 aaaggtcgcc gtggctgggg taaagaggcg ctggaacgat atgcgccaga gtatgccaac
     5101 accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg ctgtgataac
     5161 gagatggata ttcatcagtt gttgacggcc gcaatggatc cgcaggagtt tgcccgcttc
     5221 agtcaggtct ggcaggaaaa cggactggat cataactggc tgccgctgcc ggtacatccg
     5281 tggcagtggc agcaaaaaat cgctaccgac ttcatcgctg attttgccga aggcaggatg
     5341 gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc
     5401 aacgccagtc gccggggcgg gctggatatc aagctgccgc tgaccatcta caacacctca
     5461 tgctaccggg ggatccctgg cagatacatc gctgccggac cactggcttc acgctggcta
     5521 caacaggttt ttgcgaccga cgccacccta gtgcaaagcg gcgcagtgat acttggtgaa
     5581 ccggctgcag gctatgtctc ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc
     5641 taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct gaaaccggat
     5701 gaaagtccgg ttctgatggc aacactgatg gagtgcgacg aaaacaatca gccgctggca
     5761 ggcgcatata tagaccgctc cgggctggac gctgaaacct ggcttacgca actgttccgg
     5821 gtggtcgtgg ttcctctgta tcacctgctt tgccgctacg gtgtcgcgct tattgcacat
     5881 ggacaaaata taactctcgc catgaaagag ggggttccac agcgtgttct gctgaaagac
     5941 ttccagggcg atatgcgact ggtgaaagaa gagttccccg aaatggactc tttgcctcag
     6001 gaggttcgtg atgttacatc ccgcctgagt gcggactact taatccatga tttgcagacg
     6061 ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct tggcgtacct
     6121 gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa aaaacatcca
     6181 caaatgtcag agcgttttgc gcttttctca cttttcaggc cacaaatcat tcgcgtggtg
     6241 ctgaacccgg taaaactgac ctggcctgat ctggatggcg gcagccgcat gctgccgaat
     6301 taccttgagg atctgcaaaa tccgctgtgg ctggtaactc aggaatatga atcatgaaaa
     6361 aaagtgtcga ttttattggt gtagggacag ggccatttaa tctcagcatt gctgcgttgt
     6421 cacatcagat cgaagaactg gactgtctct tctttgatga acatcctcat ttttcctggc
     6481 atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa gatctggtca
     6541 gtgctgttgc acctacaaat ccctacagtt ttgttaacta tctggtgaag cacaaaaagt
     6601 tctatcgctt ccttacaagc agactacgta cagtatcccg tgaagagttt tctgactacc
     6661 tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt gaaaacattg
     6721 atttcgataa aaaacgtcga ttgtttctgg tgcaaaccag ccagggagaa tattttgccc
     6781 gcaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg aagcatatga
     6841 cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctt agtggaaaac
     6901 ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat gcattacgcg
     6961 gggaatgggg agaagcggcg gaaataaact gggtctcccg gcgtaataat tttaacgcac
     7021 tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca ggcttctccg
     7081 gactggagga agatattcgc catcagttac tggatgagca gaaaatgaca tcggatggca
     7141 tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt gaagttctga
     7201 gaaaaccaag aaatatccgt ctgctaccca gccgctcggt aacaactctg gaaagtagtg
     7261 gtcctggctg gaagttgctg atggagcatc atctggatca gggcagggag agcctggaaa
     7321 gtgatgtggt gattttcgcc acaggttacc gttctgcgtt gccacaaata cttccctcac
     7381 tgatgcccct gatcaccatg cacgataaga acacctttaa agtgcgtgat gacttcactc
     7441 tggaatggag tggcccgaaa gagaacaaca tcttcgtggt caacgccagt atgcaaaccc
     7501 atggcatcgc cgaaccccag ctcagcctga tggcatggag atctgcacgt attcttaatc
     7561 gcgtaatggg acgtgattta ttcgatctca gtatgccgcc cgccctgatt cagtggcgca
     7621 gcggcaccta gggaaaacgc agccggaggc tgcttcttta actcgctaca cagcatcttt
     7681 gggctgattt tttccgcccg tatggaggaa taatgatgat aagcaaaaag tatacgcttt
     7741 gggctctcaa cccactgctt cttaccatga tggcgccagc agtcgctcaa caaaccgatg
     7801 atgaaacgtt cgtggtgtct gccaaccgca gcaatcgcac cgtagcggag atggcgcaaa
     7861 ccacctgggt tatcgaaaac gccgaactgg aacagcagat tcagggcggc aaagagctta
     7921 aagacgcact ggctcagctg atccctggcc ttgacgtcag cagccggagc cgcaccaact
     7981 acggtatgaa tgtgcgtggc cgcccgctgg tcgtgctggt tgacggcgtg cgtctcaact
     8041 cttcacgtac cgacagccga caactggact ctatagatcc ttttaatatc gaccatattg
     8101 aagtgatctc cggtgcgacg tccctgtacg gcggcggcag taccggtggc ctgatcaaca
     8161 tcgtgaccaa aaaaggccag ccggaaacca tgatggagtt tgaggctggc accaaaagtg
     8221 gctttagcag cagtaaagat cacgatgaac gcattgccgg agctgtctcc ggcggaaatg
     8281 agcatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg tttgacggta
     8341 acggcgatgc caccttgctt gataacaccc agaccggcct gcagtactcc gatcggctgg
     8401 acatcatggg aactggtacg ctgaacatcg atgaatcccg gcagcttcag ttgatcacac
     8461 agtactataa aagccagggc gacgacgatt acgggcttaa tctcgggaaa ggcttctctg
     8521 ccatcagagg gaccagcacg ccattcgtca gtaacgggct gaattccgac cgtattcccg
     8581 gcactgagcg gcatttgatc agcctgcagt actctgacag cgcttttctg ggacaggagc
     8641 tggtcggtca ggtttactac cgcgatgagt cgttgcgatt ctacccgttc ccgacggtaa
     8701 atgcgaacaa acaggtgacg gctttctctt cgtcacagca ggacaccgac cagtacggca
     8761 tgaaactgac tctgaacagc aaaccgatgg acggctggca aatcacctgg gggctggatg
     8821 ctgatcatga gcgctttacc tccaaccaga tgttcttcga cctggctcag gcaagcgctt
     8881 ccggagggct gaacaacaag aagatttaca ccaccgggcg ctatccgtcg tatgacatca
     8941 ccaacctggc ggtcttcctg caatcaggct atgacatcaa taatctcttt accctcaacg
     9001 gtggcgtacg ctatcagtac actgaaaaca agattgatga tttcatcggc tacgcgcagc
     9061 aacggcagat tgccgccggg aaggctacat ccgccgacgc cattcctggc ggctcagtcg
     9121 attacgacaa cttcctgttc aacgccggtc tgctgatgca catcaccgaa cgccagcagg
     9181 catggctcaa cttctcccag ggcgtggagc tgccggaccc gggtaaatac tatggtcgcg
     9241 gcatctatgg tgctgcagtg aacggccatc ttcctctaac aaagagtgtg aacgtcagcg
     9301 acagcaagct ggaaggcgtg aaagtcgatt cttatgagct gggctggcgc tttactggca
     9361 ataatctgcg tacccaaatc gcggcctact attcgatttc tgataagagc gtggtggcga
     9421 ataaagatct gaccatcagc gtggtggacg acaaacgccg tatttacggc gtggaaggtg
     9481 cggtggacta cctgattcct gatactgact ggagtaccgg agtgaacttc aacgtgctga
     9541 aaactgagtc gaaagtgaac ggtacctggc agaaatacga tgtgaagaca gcaagcccat
     9601 caaaagcgac agcctacatt ggctgggcac cggacccgtg gagtctgcgc gtgcagagca
     9661 ccacctcctt tgacgtgagc gacgcgcagg gctacaaggt cgatggctat accaccgtgg
     9721 atctgctcgg cagttatcag cttccggtgg gtacactcag cttcagcatt gaaaacctct
     9781 tcgaccgtga ctacaccact gtctgggggc agcgtgcacc actgtactac agcccgggtt
     9841 acggcccagc gtcactgtac gactacaaag gcaggggccg aacctttggt ctgaactact
     9901 ctgtgctgtt ctgaccggta ttcctttaca acaaaggtac gctgatatca acatggccgc
     9961 tgacagccaa gttgatatca tataatacac gacataatct gtagtcaggg aggatagact
    10021 ctttactgac tacagattat gtcgtgttcc gtgctcattt cctcaaaaaa atacaagaaa
    10081 agaattagta ttctaacaaa aagtgaaata aattgtatca aactccctct tttaatcctg
    10141 ttgagtaaat cagcttttgc aataggattg aaagagtgta agtggaatct cttccggata
    10201 ctcgttacca ccgtggctag aatatctacg gctgcgggcc agtaatagat taagtgataa
    10261 cagctgtctg gaaatgtagg ggcgtatcca ctaatcataa tcattgtgag ataaatatta
    10321 cggggcattt tataatgaat aa
//
LOCUS       DACAPB010000160        10319 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.160, whole genome shotgun sequence.
ACCESSION   DACAPB010000160 DACAPB010000000
VERSION     DACAPB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.160"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..89
                     /locus_tag="IT904_003640"
     CDS             <1..89
                     /locus_tag="IT904_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAP3193369.1"
                     /translation="AVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            86..886
                     /gene="thiD"
                     /locus_tag="IT904_003641"
     CDS             86..886
                     /gene="thiD"
                     /locus_tag="IT904_003641"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP3193370.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            951..1769
                     /locus_tag="IT904_003642"
     CDS             951..1769
                     /locus_tag="IT904_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAP3193371.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIIAIYPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGMLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            1821..2567
                     /locus_tag="IT904_003643"
     CDS             1821..2567
                     /locus_tag="IT904_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3193372.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(2541..3506)
                     /locus_tag="IT904_003644"
     CDS             complement(2541..3506)
                     /locus_tag="IT904_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP3193373.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(3503..4507)
                     /locus_tag="IT904_003645"
     CDS             complement(3503..4507)
                     /locus_tag="IT904_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP3193374.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(4504..5781)
                     /locus_tag="IT904_003646"
     CDS             complement(4504..5781)
                     /locus_tag="IT904_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAP3193375.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            6038..7090
                     /gene="fbaB"
                     /locus_tag="IT904_003647"
     CDS             6038..7090
                     /gene="fbaB"
                     /locus_tag="IT904_003647"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAP3193376.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            7398..8252
                     /gene="gatY"
                     /locus_tag="IT904_003648"
     CDS             7398..8252
                     /gene="gatY"
                     /locus_tag="IT904_003648"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAP3193377.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            8281..9543
                     /gene="gatZ"
                     /locus_tag="IT904_003649"
     CDS             8281..9543
                     /gene="gatZ"
                     /locus_tag="IT904_003649"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAP3193378.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLALETVVERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVDTMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            9553..10005
                     /gene="gatA"
                     /locus_tag="IT904_003650"
     CDS             9553..10005
                     /gene="gatA"
                     /locus_tag="IT904_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAP3193379.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            10036..>10319
                     /gene="gatB"
                     /locus_tag="IT904_003651"
     CDS             10036..>10319
                     /gene="gatB"
                     /locus_tag="IT904_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAP3193380.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEALQNKILTILQG"
BASE COUNT         2648 a         2567 c         2680 g         2424 t
ORIGIN      
        1 gcgcagtcgc cagaagcgag gggccaggca gttttgttcc acatttcctt gatgcgctct
       61 ggcaattgac gcaggaggtg caggcatgaa acgaattaac gctctgacga ttgccggtac
      121 tgatccgagt ggtggtgcgg ggattcaggc cgatcttaaa accttctcgg cacttggcgc
      181 ttatggttgc tcagttatta ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt
      241 gtatcgcatt gagcctgatt ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg
      301 aatcgatacc actaaaatcg gtatgctggc ggaaaccgat attgttgaag cggtggcaga
      361 acggttgcaa cgttatcaga tccaaaacgt ggtactcgac accgttatgc tggcaaaaag
      421 cggcgacccg ctgctttcac cttcggcggt tgctacgctg cgcagtcgat tattgccaca
      481 ggtttcatta ataacgccaa acttgcccga agctgccgcc ttgctcgacg cgccacacgc
      541 gcgcaccgaa caggaaatgc tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc
      601 agtgctaatg aaaggtggtc atctggatga tgagcaaagc ccggactggc tgtttacccg
      661 cgagggtgaa caacggttta ccgcaccgcg cattatgacc aaaaacaccc acggcactgg
      721 ttgtacactc tctgcggcgt tggctgcact acgcccgcgc catacaaact gggctgacac
      781 cgtacaggag gcaaaaagct ggctttcatc ggcgttagcc caggccgaca cgctggaagt
      841 tggtcacggt attggtccgg ttcaccactt ccacgcctgg tggtgagcac cgtactatag
      901 ctgctttggt tattgtcagc ctttggcact gacattcagg gatgaaacca atgcagctaa
      961 gaattaccag tcgaaaaaaa ctcacctctc tattatgcgc tcttgggcta atttccatca
     1021 ttgcaattta tccgcgtcag acggtgaatt ttttctattc gacagcagtt cagattaaag
     1081 actacatcca cttctacggt tatcgcccgg ttaaatcttt cgcaattcgc attcctgcca
     1141 gttataccat tcacggaata gatgtttcgc gctggcagga gcggatcgac tggcagcggg
     1201 tggcaaaaat gcgcgacaac ggtatccgct tacagtttgc ttttattaag gcgacggaag
     1261 gcgaaaagct ggtggacccc tatttttcgc gtaactggca actaagccgc gaaaatggca
     1321 tgctgcgtgg ggcgtatcat tatttctccc cgtcggtatc cgcttcagtt caggcgcgat
     1381 tatttctgca aacggtggat ttctcacaag gcgatttccc tgccgtgctg gacgtagaag
     1441 aacggggaaa attatcggca aaagaattac gcaagcgggt aagtcagtgg ctaaaaatgg
     1501 tagaaaaacg cacgggaaaa aagccgatta tttactcagg agccgttttt tatcacacga
     1561 atctggcggg ctattttaat gaatatccgt ggtgggtggc tcactattat caacgtcgtc
     1621 cggacaatga cggaatggcc tggcgcttct ggcagcattc cgaccgtgga caggtagatg
     1681 gcatcaatgg tccggtggat tttaatgtgt ttaatggcac ggtggaggag ttgcagggat
     1741 tcgttgatgg gattaaagaa acgccttaaa taagaagcag tgatatactc ccaccagact
     1801 ccctggcagg aaaaagacaa atggaacaag cgcataccca gctgattgcc caactgaacg
     1861 agcgtatttt agctgcggat aacacgccgc tttatattaa gtttgccgaa acggtaaaaa
     1921 atgccgtgcg cagcggggtg ctggagcatg gcaatatttt gcccggtgag cgtgatttaa
     1981 gtcagttaac cggcgtttcg cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag
     2041 agggtgtggt gacgcgttcg cgcggttacg gcacgcagat caacaacatc ttcgaatact
     2101 cgttgaaaga agcgcgaggt ttttctcagc aggtggtatt gcgcgggaaa aagcccgata
     2161 cgctatgggt taacaagcgt gtcgtgaaat gtcccgaaga agtcgcgcag cagctggcgg
     2221 tcgaagcagg aagtgatgtc tttttgctta agcgtattcg ctatgtcgat gaagaagcgg
     2281 tatcgattga ggaatcgtgg gttccggcgc atttaattca tgatgttgat gccatcggga
     2341 tttcacttta tgactatttc cgcagccagc acatttaccc acaacgtaca cgttcccgcg
     2401 ttagcgcccg gatgccggat gccgagtttc agtcacatat tcagttagat agcaaaatac
     2461 cggtgctggt gatcaagcaa gtggcgcttg accaacagca acggcctatt gagtacagca
     2521 ttagccactg tcgcagcgat ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt
     2581 ggcgcacaat caccgccccg atgcccgaca acccacgacg ccactgcatt gcccagtaat
     2641 acggcatccg ccagtggcag accagaggcc agcccggcaa gtacgccacc tgcatgactg
     2701 tcgcccgccc caatggtgtc tacaacttgc gttggaaatg ccggaatgca gccagaagcg
     2761 tcgttgctga aataccatgc accctcttta tcgagacgaa cgatcaacgg cgcggcaaat
     2821 ttctcctgcc attgcttgcc aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg
     2881 gcaatctcag cctcttgacg attgagtgat actaaaggtc gacaggccat gatccgcgcc
     2941 agtaatgcgt ccgggatatc gccgatacgt gggccaaaat cgataaacgg cgtcacgtct
     3001 tgcagctttt ccagccattc cactaacaat tcgccgcagg gcgaggccag ttgataaccg
     3061 gaaaaataga gcaggctgcc aggcgcaacg gttaatcgcg ccagccactg gcgattccac
     3121 tgattttcca caccgctgaa tgacataaaa gtgcgttcgc catccggctc aaccagcgcc
     3181 agacaccagc cgttatcgcc ttcggcgtta tcgatcaggc tgattaaccc ctctttcgcc
     3241 atccggttgc gaataatctc cgcccacacg ccctgaccga gcggcaaggc attacccgct
     3301 tcgatgccga ggcgctttaa cgccacggca atattcagtg cgcagccgcc aacgttaacg
     3361 ctctgctgct tcagttcgat atcgcaccca cgccagggga gggcataagc gtcggcgatc
     3421 acatcaatga ccgccgcgcc gacgaccatc accggctgac gcgaggttaa ttcaggcagc
     3481 agcgtgtgta atcgagcgcc gctcataccg cctcccgttg ttgacgatat ttcgccagcg
     3541 ctgtggcata gcggttgaaa tcaagctgat ttaccgcatc cagttccgcc tttaatgcag
     3601 gatcgatagc gttaacgcca tgcaacgcgc cgcaaatcgc cgtcgccata gcgccgatgg
     3661 tgtctgtgtc gccgccaagg ttagcgcaca ggacggcgca gcgattcggg tcggtttgtg
     3721 ccagttcaac cagcgcaatg gcgcacggaa cggactcaat agtgctggta cctgcgccaa
     3781 cgacctggta aagctgttcg ctggcggatt cggtgccgtc ggcattgcgc acaattttca
     3841 gcgcaatctc cagacgtgct gccagtgagg cgctgaaggt agtgatgcgt ttttgctgtg
     3901 catgtcgcgc aattgaaggc agtgaatcta caatcgctga ccagctttct ccgtcaatgg
     3961 cacgagaaat cgcccatgcg atgactaccg cgcctgcaac cgccagatcg gatttatggg
     4021 tcgggctgga cgccagcgcc acatcatcaa taaaggaatc aacatcgtgc gccggaagca
     4081 aacaacctaa tggcgagacg cgcatcgctg cgccgttggt cacgccgttg ttttccagtt
     4141 cagcaatggg tttaccgtcg cgaatggcgt taagcgcaat cttcgaggtc ggaccaagta
     4201 cgtttttgtt aaaggcgtcg aaacgcagcg cccagtcgag aatattacgc ccaatcagat
     4261 tcggatcgat cttgccttca cgttccagta acgcatccgc cagacacagc gccatcgagg
     4321 tatcgtcggt gaattcggcg cggttaaaat aacaggccgc gttattctcc tttggtccag
     4381 gaagaaaacg gtcaatccag ccaaagtgtg ctttaacgcg gctgcgtggc caaagctcgg
     4441 agggcatccc catcgcatcc cctaacgcct gcccataaag agcaccgaga atacgttctg
     4501 ttttcattta acttcccctt gtgtcaacgc aatatcgcga tcatcgacct tgatagccgt
     4561 aatttcgttg tcggattcgc ggaaaaaaat catgaacagc acggcgataa tggcaatcat
     4621 caccgcgccg aaagtccaca tcccggacca gttgaaagtc agtccgttta ccggttcctg
     4681 ataagcgaac atcttttcca tcatcacacc gccaagacga tagccgagca aactgccgaa
     4741 gccctggcag cagagcgtga tcagcccctg cgcagcggta cgcatatgca cgggggcttt
     4801 tttatcgaca tagatgtaag cggtaacgta gtaaaaatcg taacttacgc cgtgaagcaa
     4861 aataccgagg aacagtaacg cgtaggtgaa atattcatcc gcactaccgt aaataaagaa
     4921 gccatagcgg atcgcagcgg tgaccagacc aagcaataat acctttttga taccaaagcg
     4981 tttagtgaaa aacggcaatg ccagcataaa gaagatttca gagaactggc cgagcgtcat
     5041 ccagccggtg gcgtttttca tgccaacttc ggtcagataa ccgttggcaa agatgtaata
     5101 gaacgctagt ggcatcgcaa acaggaatga acagaagaaa aagacgagga agtttttatc
     5161 gcgcagcagg atcagcgcat ccaggccgag catgacttta atatccattt tgccggtgct
     5221 ttttggtggc gtgtcgggca ggaaaaacgc aaacacaccg agcagagcag aacttccggc
     5281 ggtaatcagc agcgggatgt tagtcggtga gatatcggca taccccagta tttgcggcaa
     5341 gaaaccacat gccagaccgg aggcaatcca gccgatagtg cccatcacac gaatgcgcgg
     5401 gaaatcacgc tcaacatccg gcacgttggc aaaagcgatg ctgttagtca gcgcaatggt
     5461 cggcatatag gttagcgagt aggccagcag taacgggaag aacccggcaa aagtggtctg
     5521 ttgcgcagcg aaatacatca gcagcgcgcc tgcgaacatc aataccgcca gcactttctg
     5581 cgccgagaaa aagcggtcag tgatggagcc aaccagaatt ggcgacagga tcgccgcaat
     5641 ggcggtacag gcatacgacc agccaatttc tccggcacta aaaccgcttt tacttaacca
     5701 caaccacaat ggcacaaacc acgcgcccca gataaaccat tcaacaaaca tcatgaacga
     5761 cagctttgct gttgttttca tcttttaatt ccttcatgac aggtaatgta aggtacgcga
     5821 tgacaatacc acttaataat accttttaaa tacctttgcg gtgaaaactt gaccgcgcta
     5881 acattttttc tgatgaatcg agccaacaga aaacgctgaa aaaacatcca aaagatggaa
     5941 aaactcgccc gtaacgacca ggattatgat tgcccgcaaa aggcgggcca ggaaaatcca
     6001 tagccgatat ccaatcggaa tttacgggag catagtaatg acagatattg cgcagttgct
     6061 tggcaaagac gccgacaacc ttttacagca ccgttgtatg actattcctt ctgaccagct
     6121 ttatctcccc ggacatgact acgtagaccg cgtgatgatt gacaataatc gcccgcctgc
     6181 ggtgttacgt aatatgcaga cgttgtacaa caccgggcgt ctggctggca caggatatct
     6241 ttctattctg ccggttgacc agggcgttga gcactctgcc ggagcttcat ttgctgctaa
     6301 cccgctctac tttgacccga aaaacattgt tgaactggcg atcgaagcgg gctgtaactg
     6361 tgtggcgtca acttacggcg tgctggcgtc ggtatcgcgg cgttatgcgc atcgcattcc
     6421 attcctcgtc aaacttaatc acaacgagac gctaagttac ccgaatacct acgatcaaac
     6481 gctgtatgcc agcgtggagc aggcgttcaa catgggcgcg gttgcggttg gtgcgactat
     6541 ctattttggc tcggaagagt cacgtcgcca gattgaagaa atttctgcgg cttttgaacg
     6601 tgcgcacgag ctgggcatgg tgacagtgct gtgggcctat ttgcgtaact ccgcctttaa
     6661 gaaagatggc gttgattacc atgtttccgc cgacctgact ggtcaggcga accatctggc
     6721 ggcgaccatt ggcgcggaca tcgtcaaaca gaaaatggcg gaaaataacg gcggttataa
     6781 agcgattaat tacggttata ccgacgatcg cgtgtacagc aagttaacca gcgaaaaccc
     6841 gattgatctg gtgcgttatc agttagctaa ctgctatatg ggccgggctg ggttgataaa
     6901 ctccggcggt gctgcgggcg gtgaaactga cctcagtgat gcagtgcgta ctgcggttat
     6961 caacaaacgc gctggcggaa tggggctgat tcttggacgt aaagcgttca agaaatcgat
     7021 ggctgacggc gtgaaactga ttaacgccgt gcaggacgtt tatctcgata gcaaaattac
     7081 tatcgcctga tacgccttat acgaacaaca ccaccccgta ggacggataa ggcgtttacg
     7141 ctgcatccgg caacctatgc ctgatgcgac gctgaagcgt cttatcatgc ctacatagca
     7201 ctgccacgta tgtttacacc gcatccggca taaaaacacg cgcactttgc tacggcttcc
     7261 ctatcgggag gccgtttttt tgcctttcac tcctcgaaca attttcatat tgtcgttttt
     7321 gtgatcgtta tctcgatatt taaaaacaaa taatttcatt atattttgaa atcgaaaaca
     7381 aacgacagga tatgaaaatg tacgtggtat cgacaaagca gatgctgaac aacgcacagc
     7441 gcggcggtta tgcggttccg gcattcaata ttcacaatct cgaaacgatg caagtggtgg
     7501 tagaaaccgc tgccaacctg catgcgccgg tcatcatcgc cggaacgcct ggcacattta
     7561 ctcatgctgg tacagaaaat ctgttggcgc tggtcagcgc gatggcgaag caatatcacc
     7621 atccactggc aattcatctc gaccatcaca cgaaatttga cgatatcgct cagaaggttc
     7681 gttctggcgt gcgctcagtc atgattgacg cctcgcattt gccttttgcg caaaatattt
     7741 cacgggtcaa agaggtggtg gatttttgcc atcgctttga tgtcagcgtc gaggcggagc
     7801 tggggcaact tggcggccag gaagatgatg tgcaagtcaa tgaagccgat gcgttgtaca
     7861 ccaaccccgc tcaggcgcgt gaatttgccg aggcaaccgg aattgattcc ctggcggtcg
     7921 ccatcggcac ggctcatggg atgtatgcca gcgcaccggc gcttgatttt tctagactgg
     7981 agaacattcg ccagtgggtg aacttaccgc tggtgctgca tggcgcgtca gggttatcga
     8041 ctaaggatat tcagcaaacc atcaaactgg ggatatgcaa aatcaacgtt gcaacggagc
     8101 tgaaaaatgc cttctcgcag gcgttaaaaa attacctgac cgagcaccct gaagcgaccg
     8161 atccccggga ttatttgcag tcggctaaat ccgcaatgcg cgatgtggtg agcaaagtga
     8221 ttgccgattg tggctgcgag ggcagggcat aacgcacctg ccatttaaca aggaaaaaac
     8281 atgaaaacgt taattgcccg gcataaagct ggtgaacata tcggcatatg ttcagtctgt
     8341 tctgcccatc cgttggttat cgaagcggcg ctggcatttg atcgcaacag cacgcgcaaa
     8401 gtgctgattg aagcaacgtc aaaccaggtc aatcaatttg gcggttatac cggaatgaca
     8461 ccggcagact ttcgcgaatt tgtttttacg attgccgata aagttgggtt tgcacgcgaa
     8521 cgcattattc tcggcggcga tcatctgggg ccaaactgct ggcagcaaga aaatgcggat
     8581 gcggcgatgg aaaaatccgt cgagctggta aaggaatatg ttcgtgccgg cttcagtaaa
     8641 attcatcttg atgcgtcaat gtcctgcgcg ggggatccca taccgttagc actagaaacg
     8701 gttgtggaac gagctgctgt gctttgcttt gctgcggaaa gtgtggcgac agattgccag
     8761 cgtgagcaac tgagctatgt cattggcacc gaagttccgg ttccgggcgg tgaggccagc
     8821 gccattcagt cagtacacat cacccatgtt gaagatgccg ccaatacttt acgtacgcat
     8881 caaaaggcct ttattgcccg tgggctgaca gaggcgttaa cacgtgtgat tgccatcgtg
     8941 gtgcagccgg gtgtggaatt tgatcacagc aatattatcc attatcagcc gcaggaagcg
     9001 cagccgctgg cgcaatggat agaaaacacc cgaatggttt atgaagcaca ttctaccgat
     9061 taccagaccc ggacggctta ttgggaatta gtccgcgatc actttgcaat attgaaagtc
     9121 ggtcccgcat taacctttgc tttacgcgag gcgatatttg cactggcaca aattgagcag
     9181 gaacttatcg cccctgaaaa tcgcagcggt tgcctggcgg taattgaaga agtgatgctc
     9241 gacgaaccgc aatactggaa aaaatattat cgtacgggtt ttaacgattc attactggat
     9301 attcgttaca gcctgtcgga tcgtattcgt tattactggc cgcatagtcg gattaaaaat
     9361 agcgtcgata cgatgatggt gaatcttgaa ggcgtggaca tcccactggg catgattagt
     9421 cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg aattatcagc aataccgcat
     9481 cagctgatta tggataaaat ttatgatgtt ttgcgcgcct atcgctacgg ctgtgcggaa
     9541 taaggacggt atatgactaa cctgtttgtt cgtagcggaa tttcttttgt cgatcgtagc
     9601 gaagttttaa cccatatcgg taatgagatg ctcgccaaag gtgtggttca tgatacctgg
     9661 ccacaggcat taattgccag agaagcagaa ttccctaccg ggataatgct tgagcagcac
     9721 gctattgcaa taccgcattg tgaggcgatt catgctaagt cgtcagccat ttatctgtta
     9781 aggccaacaa ataaagttca ttttcagcaa gcggatgatg ataacgacgt ggcggtatcg
     9841 ttggttattg cgttgattgt ggaaaatccg cagcagcaat tgaaactttt acgctgttta
     9901 tttggcaagt tacaacagcc cgatatcgtc gagacactaa tcactcttcc tgaaacccag
     9961 ttaaaggaat acttcacaaa gtatgtttta gattcagacg aataaatccc tctgtaacaa
    10021 taaaaaggac tatttatgaa acgcaagatt attgtcgctt gcggaggcgc ggttgcgacc
    10081 tctacgatgg cggcggaaga aattaaagag ttgtgtcaga atcataatat tcctgttgaa
    10141 ttaatccagt gtcgggttaa tgaaatagaa acctatatgg atggtgtgca tttgatatgc
    10201 accactgcca aagtggatcg tagttttggc gatattccgt tagttcacgg catgcctttt
    10261 atttctggta tcggtatcga agcattacaa aataaaattc tgactatctt acaggggtg
//
LOCUS       DACAPB010000161        10164 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.161, whole genome shotgun sequence.
ACCESSION   DACAPB010000161 DACAPB010000000
VERSION     DACAPB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.161"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(62..1072)
                     /gene="ruvB"
                     /locus_tag="IT904_003652"
     CDS             complement(62..1072)
                     /gene="ruvB"
                     /locus_tag="IT904_003652"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP3193381.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1081..1692)
                     /gene="ruvA"
                     /locus_tag="IT904_003653"
     CDS             complement(1081..1692)
                     /gene="ruvA"
                     /locus_tag="IT904_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP3193382.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(1831..1896)
                     /locus_tag="IT904_003654"
     CDS             complement(1831..1896)
                     /locus_tag="IT904_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193383.1"
                     /translation="MYIFITHFFTEYVILKYLLPK"
     gene            1967..2569
                     /locus_tag="IT904_003655"
     CDS             1967..2569
                     /locus_tag="IT904_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAP3193384.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(2571..3092)
                     /gene="ruvC"
                     /locus_tag="IT904_003656"
     CDS             complement(2571..3092)
                     /gene="ruvC"
                     /locus_tag="IT904_003656"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAP3193385.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(3127..3867)
                     /locus_tag="IT904_003657"
     CDS             complement(3127..3867)
                     /locus_tag="IT904_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP3193386.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(3896..4348)
                     /gene="nudB"
                     /locus_tag="IT904_003658"
     CDS             complement(3896..4348)
                     /gene="nudB"
                     /locus_tag="IT904_003658"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAP3193387.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(4466..6238)
                     /gene="aspS"
                     /locus_tag="IT904_003659"
     CDS             complement(4466..6238)
                     /gene="aspS"
                     /locus_tag="IT904_003659"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP3193388.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            6548..7114
                     /locus_tag="IT904_003660"
     CDS             6548..7114
                     /locus_tag="IT904_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP3193389.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            7111..7929
                     /locus_tag="IT904_003661"
     CDS             7111..7929
                     /locus_tag="IT904_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP3193390.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            7982..8377
                     /locus_tag="IT904_003662"
     CDS             7982..8377
                     /locus_tag="IT904_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193391.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            8418..9161
                     /gene="cmoA"
                     /locus_tag="IT904_003663"
     CDS             8418..9161
                     /gene="cmoA"
                     /locus_tag="IT904_003663"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAP3193392.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            9158..10129
                     /gene="cmoB"
                     /locus_tag="IT904_003664"
     CDS             9158..10129
                     /gene="cmoB"
                     /locus_tag="IT904_003664"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAP3193393.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIARKP"
BASE COUNT         2391 a         2693 c         2517 g         2563 t
ORIGIN      
        1 cgaatagcgt cgcatcaggc acactgccaa tttcggatgc ggtgcgagcg accaatccga
       61 cttacggcat ttctggcggc gttatgccaa agtgattcca cgcccgcgtc gtcgccatac
      121 gcccacgcgg tgtacgctgc aaaaagcctt gctgaatcaa ataaggttcc agcacatcct
      181 caatggtttc acgttcttcg ccaatggctg ccgcaaggtt atccagacct accggcccac
      241 caaagaactt atcgattacc gccagcaata acttgcggtc catataatcg aaaccttcag
      301 catcgacatt caacatatcc aacgcctgag cagcgatatc tgccgagatg gtgccatcgt
      361 gcttcacttc ggcgaaatca cgcactcgac gcagcagacg gttggcaatg cgcggcgtac
      421 cgcgagcgcg acgagcaact tccagcgccc cgtcatcact catctcaagc cccataaagc
      481 gtgcgctgcg actgacgata tattgcagat ccggcacctg ataaaactcc agacgttgca
      541 caatcccaaa acggtcgcgc aacggtgatg tcagcgaacc tgcgcgcgtg gttgcaccaa
      601 tcagggtaaa cggcggcaaa tcaattttaa tggagcgtgc cgccggacct tcaccaatca
      661 tgatatccag ttggtagtct tccattgccg ggtacagcac ttcttcaaca actggcgata
      721 aacggtggat ctcatcaata aacagcacgt catgcggttc aaggttagtg agcatcgcag
      781 ccaaatcgcc cgccttttcc agcaccggac cagaagtcgt gcgtaaatta acgcccattt
      841 cattggcgac aatgttggca agcgtagttt tacccaaccc cggaggacca aaaatcaaca
      901 aatgatcgag ggcatcgccg cgcagtttcg ctgctttgat gaaaatctcc atctgtgaac
      961 gaacctgcgg ctgaccaaca tactcttcca gtaatttagg gcgaatggcg cgatctgcta
     1021 catcttccgg caaagtggta ccggcagaaa tcagacggtc tgcttcaatc atcctttacc
     1081 tcataacgcg gcgcgtaggg cttcgcgaat taatgtttca ctgctggcgt cagggcgagc
     1141 gattttgctc accatgcggc tggcttcttg tggtttatag cccagcgcca ccagcgcagc
     1201 aaccgcttcc tgttcagcat cgttggttgc cgggctggca ggcgacgtga gcaccagatc
     1261 ggcggctggc gtaaagagat cgccatgcag acctttaaat cggtctttca tttcaacaat
     1321 caagcgttcg gcggtttttt tgccaatacc cggcagtttc accagcgccc ccacttcttc
     1381 acgctcaacg gcattaacga actgctgcgc tgacattccg gagaggatcg ccagcgccaa
     1441 cttcgggccg acgccgttgg ttttgatcaa ctctttgaac aacgtgcgct cttgtttatt
     1501 gttaaaaccg tacagcagct gcgcgtcttc acgcaccaca aagtgggtga aaacgatcgc
     1561 ttcctgtccc gcttcaggga gttcataaaa acaggtcatc ggcatatgca cttcatagcc
     1621 tacgccgccc acttcaatta acaccagcgg gggttgtttt tcaatgatga tgcctctgag
     1681 tctgcctatc acatgacgct cctgcgtaat gaatcaaaga taatgctgta tgataaaaaa
     1741 atgctggata gatatccagc gaaggatgaa gaaaacttgc gaggtgtctc gatgatctga
     1801 aaaatggcac agtataattt attctacagg ttattttgga agcaaatatt ttaatattac
     1861 atattcagtg aaaaaatgcg taataaaaat atacattgcg cctcctgaaa aaataaattt
     1921 tttatgctat tacgtatatt cgtatctatt tcaatggaat gacaacgtga atattaatta
     1981 ccctgctgaa tatgaaattg gagatatcgc ctttacatgt ataggtgctg ccttatttgg
     2041 tcaaatatca gctgcatcaa attgttggag taatcacgtc gggatcatta tcggtcataa
     2101 cggtgaagac ttcctggttg cagaaagccg ggttcccctc tcaaccatca ctacgctatc
     2161 ccgttttatt aaacgctctg ctaatcaacg ctatgctata aagcgattag acgctgggct
     2221 gacagaacaa caaaaacaac gaattgttga acaggttccc tcccggctac gcaaacttta
     2281 ccacaccggt tttaaatacg aatcttcgcg ccagttctgt tcaaaatttg tctttgatat
     2341 ttataaagag gcgctatgta ttccggtggg tgaaatagag acgtttggag aattgttaaa
     2401 tagcaatccg aatgcaaaac tcactttctg gaaattctgg ttcctgggtt ctattccgtg
     2461 ggagcgtaaa accgttacgc cagccagttt gtggcatcat ccgggtttgg tgttgattca
     2521 tgcggaggga gtggaaacgc ctcagccgga actgaccgag gcggtataac ttaacgcagt
     2581 cgccctctcg ccaggttcag ccgcgattcg ctcatctgca tcgcattctg actaacgtgg
     2641 cagtgggtga tggcaatcgc caacgcatca gcggcatccg cctgtggatt agcgggcagt
     2701 ttcagcaagg tgcggaccat atgctgcacc tggctttttt cggcgctacc aatacccacc
     2761 accgtttgct tcacctgacg tgcagcatat tcaaataccg gcaactcctg attcaccgcc
     2821 gccacaatcg ccacgccgcg cgcctggccc agtttcaggg ctgagtcagc gttctttgcc
     2881 ataaagactt gttcaatggc gaaataatca ggctggaact gggtgatgat ttccgtcacg
     2941 cccgcataga tgagtttcag acgagacggt aaatcatcca ctttggtgcg tatgcatccg
     3001 ctacccaggt aggacagttg cctacctacc tggcggatga cgccgtagcc ggtcacgcgc
     3061 gaacccggat caatgccgag aataatagcc atcacgcgtc tccgttttgc tgtttagcag
     3121 gcctcatcag agagtcgctg cgacctcatc agagatttca ccgttatggt aaacttcctg
     3181 cacgtcgtcg caatcttcca gcatatcgat cagacgcatc agtttcggtg cggtttctgc
     3241 atccatatca gctttggtag acgggatcat ggaaacttcc gcgctgtctg ctttcagacc
     3301 tgccgcttcc agagcgtcgc gcactttacc catttcttcc catgcagtgt agacatcaat
     3361 cgcgccgtca tcataggtca caacgtcttc agcacctgct tccagtgctg cttccatgat
     3421 ggtgtcttca tcgcctttct cgaaggagat cacgcctttt ttgctgaaca gataagctac
     3481 ggaaccatca gtaccgaggt taccgccaca tttgctgaat gcatgacgca cttcagcaac
     3541 ggtacggttg cggttgtcag acagacattc aatcatgatt gccgtgccgc caggaccgta
     3601 accttcgtag atgatggttt ccatgtttgc atcatcatca ccgcccacac cacgtgcaat
     3661 tgcgcggttc agtgtgtcgc gggtcatgtt gttagacagt gctttatcaa ttgctgcacg
     3721 cagacgcggg ttagcgtccg gatcgccacc gcccagctta gccgcggtta ccagctcacg
     3781 aatgatttta gtgaagattt taccgcgctt agcatcctgc gcagctttac gatgtctggt
     3841 gttggcccat ttactatgac ctgccataaa aatatctcca gatagccctg cctgttcagg
     3901 cagcgttaat tacaaactgt tcaatcgcct gccggttgct ccaggactta gtgagcgccg
     3961 ccgcagcagg cgcatcaagc cacttgtaag ccagatgttc agtgaaaacg atctgccgct
     4021 cgtgcggaag cgcaagacag aaccatgatt ccgtattacg cgtcacgccc ggcgcatagc
     4081 gatgacgtaa atgtgaaaaa atttcaaact ctaccgtgcg ctgacagtca attaaggtca
     4141 gttgttcagc gacaacatca atggtgacct cttcctttac ttcgcgcatg gcagcttgcg
     4201 gcgcggtttc accctcttcc acgctgccgg ttaccgactg ccagaaatcg ggatcgtcac
     4261 gccgctgcaa catcagcacc cgtttcgtat cttgtgcgta gatgaccact aagatcgaaa
     4321 cgggacgctt atacacttta tccttcacgc tgcctcttcg ttgactgcct tcgctcatcc
     4381 cattcacata gttatctatg ctcatgggag ttcactcagt tgccgcctcg atgcaacgcg
     4441 aatgatttcg tgtatttgag tcatatcagt tattctcagc cttcttcaca acctgaatgc
     4501 tcagctcagc cagtgcagtc gggttagcaa agctcggtgc ttcagtcatc agacacgctg
     4561 ccgccgtggt tttcgggaag gcgataacgt cacggatatt gtcggtgccg gtcagcagca
     4621 tggtcagacg gtcaagaccg aatgccagac ctgcgtgcgg cggagtaccg tatttcagag
     4681 cgtcgagcag gaagccgaat ttctcgcgct gttcctcttc gttgataccc agaataccaa
     4741 acaccgtctg ctgcatatca ccattatgga tacgtactga accaccgccc acttcgtaac
     4801 cattgatgac catatcgtaa gcgttcgcca ccgcattttc cggtgcagct ttcagttctg
     4861 cagccgtcat atctttcggt gaggtgaacg gatggtgcat tgccgtcagg ccgccttcac
     4921 cgtcgtcttc aaacatcggg aagtcgataa cccacagcgg tgcccatttg ctttcgtcgg
     4981 tcagaccaag gtctttaccc actttcaggc gcagtgcacc catcgcgtcg gcaacaattt
     5041 tcttgttgtc ggcaccgaag aaaatcatat cgccatcttg cgcggcagta cgatccagga
     5101 tgtcttcgat gatttctgca ttaaggaact tcgctaccgg gctgttgata ccttccagac
     5161 ctttcgcgcg ttcgttaact ttgatgtaag ccagaccttt cgcgccgtag attttaacga
     5221 agttaccgta ttcgtcgatc tgcttacggg tcagcgatgc gccgcccgga acgcgcagag
     5281 ccgctacgcg acctttcgga tcgttcgccg gacctgcaaa tacagcaaac tcaacagatt
     5341 tcagcagatc agcaacgtca gtcagttcca tcgggttacg cagatccggt ttatcagaac
     5401 cataacggcg ttctgcttcc gcaaaggtca ttaccgggaa atcgcccaga tccacaccct
     5461 tcacttccag ccacagatga cgcaccagcg cttccatcac ttcacgcact tgcggcgcgg
     5521 tcatgaaaga agtttccaca tcgatctgag taaattcagg ctgacggtca gcacgcaggt
     5581 cttcgtcacg gaagcattta acgatctgat agtaacggtc aaaaccggac atcatcagca
     5641 gctgtttgaa caactgcggg gattgcggca gtgcgtagaa tttacctttg tgcacacgag
     5701 aaggcaccag gtagtcacgc gcgccttccg gcgtggcttt ggtcagcatc ggagtttcga
     5761 tgtcgaggaa gccgtggtca tccataaaac ggcgcaccag gctggtgatt ttagcgcggg
     5821 ttttcaggcg ctgagccatt tccggacgac gcaggtcgag gtagcggtat ttcagacgcg
     5881 cttcttcggt gttgacgtgg ttagagtcaa gcggcagaac atctgcgcgg ttgatgatag
     5941 tcagcgagga cgccagcact tcgatttcgc cggtcgccat atcgcggtta atattttttt
     6001 cgtcacgcgc acgtacggtg cccgtgacct gaatgcagaa ctcattacgc agttcagagg
     6061 ccagctttaa cgcgtccgca cgatccggat cgaaaaatac ctgcacgata ccttcgcggt
     6121 cgcgcatatc gatgaagatc aggctaccaa gatcacgacg acggttgacc caaccacaca
     6181 gagtcacctg ctgccccacg tgggacaaac ggagctgtcc acaatattct gtacgcatga
     6241 gatatccctt aacttagctg ccggcggatg ccccctgctg cgcaggtgac caagtcgcag
     6301 cgttagctgt atgtcacaac tgaatgaaaa aaggcggcta ttatactgga aattctgccg
     6361 caccgtaaga gcctggcccg cgctggaacg cctcgttacc actttatatc gggcctgaaa
     6421 tcagactcta cgccagtttg ctataaaggt gttgcccgaa ctcataaaaa ttaacaaaat
     6481 ttgtcgttcc gccatcggct aatcgcatta aggtgtgagg cacgattttg ttttttcagg
     6541 agtcattatg cttgaactta atgctaaaac cactgcgctg gtggtgatcg atttacaaga
     6601 aggcatctta ccttttgccg gtggtccaca tactgccgat gaggtagtta atcgcgccgg
     6661 gaagctggcg gcgaaatttc gcgccagcgg tcagcccgtg tttctggtgc gcgttggctg
     6721 gtctgccgat tacgctgaag cattaaaaca gccggtcgat gcgccctcac cggctaaagt
     6781 gttgcccgaa aattggtggc aacatcctgc tgcattaggt gcaaccgaca gcgatatcga
     6841 aatcatcaaa cgtcaatggg gtgcgtttta cggtacggat ctggagttgc aattacgccg
     6901 ccgaggtatc gatacaatag tgttatgtgg gatctcgacc aatatcggtg ttgaatccac
     6961 cgcccgcaat gcctgggaac ttggctttaa tctggtgatt gccgaagacg cctgtagtgc
     7021 cgccagcgcc gagcagcaca ataacagtat caaccatatc tacccgcgca tcgcccgtgt
     7081 gcgtagcgtg gaagagatcc tcaacgcgtt atgatttaca tcggtctacc gcaatggtcg
     7141 catcctaaat gggtgcggtt ggggatcacc agccttgaag agtatgcccg ccactttaac
     7201 tgcgtggagg gcaacaccac gctttacgcc ctgccgaaac ccgaggttgt cctgcgctgg
     7261 cgtgagcaga ccacagatga cttccgcttc tgttttaagt ttccggcgac catttcgcat
     7321 caggcagcat tacggcattg cgatgattta gtgactgaat ttttgacccg catgtcaccg
     7381 ttggctccgc gcattgggca atactggctg caactgcctg ccacattcgg cccacgggag
     7441 ctgcctgcgc tttggcattt tctcgattct cttcctggcg aatttaatta tggcgtggaa
     7501 gtccgccatc cacagttttt cgccaaagga gaagaggaac aaacgcttaa tcgcggttta
     7561 catcagcgcg gcgttaatcg ggtgatttta gacagccgcc cggttcatgc agcacgtcca
     7621 cacagtgaag ctattcgcga cgctcaacga aaaaaaccta aagttccggt acatgctgta
     7681 ctgacggcga caaatccgct gatccgtttt atcggtagtg atgatatgac gcaaaaccgg
     7741 gaattatttc aggtctggtt acaaaaatta gcgcagtggc atcagaccac tacgccttat
     7801 ctttttttac atacgccaga cattgcccag gcaccggaac tggtacatac cctgtgggaa
     7861 gacttacgta aaacgcttcc agagatcgga gcagttccgg ctattccaca gcaatcttct
     7921 cttttctgaa tttgccacct atcatagaca ggtgccatcg gccattttaa agggagtttg
     7981 tatggtaagc gcgctgtatg ccgttttaag tgcgttgtta ttaatgaagt tctcttttga
     8041 tgtcgttcgc ctgcgaatgc agtaccgcgt tgcctatggc gacggcggtt ttagcgaact
     8101 gcaaagcgct attcgcattc atggtaacgc ggtggaatat attcccatcg cgattgtgct
     8161 gatgctgttt atggaaatga atggcgcaga aacctggatg gtgcatattt gcggcatcgt
     8221 tttgcttgct ggtcgtctga tgcattatta cggttttcat caccgtctgt tccgctggcg
     8281 acgttccggc atgagcgcca cctggtgtgc gctgttgctg atggtgctgg cgaatctttg
     8341 gtatatgccc tgggagttgg ttttctccct gcgttagcgc acaatacgcc actttctttt
     8401 tcccggattt ttacgttatg tctcaccgcg acacgctatt ttctgcccct atcgccagac
     8461 tgggcgactg gacctttgat gaacgggtag ctgaagtctt cccggatatg atccagcgtt
     8521 ccgtacccgg ctattccaat attatttcca tgattggtat gttagccgag cgcttcgttc
     8581 aacctggtac gcaggtttac gatctgggtt gttctctggg cgcggcgacg ctctcggtgc
     8641 gtcgcaacat tcatcatgat aattgcaaaa ttattgccat cgacaactcc ccggcgatga
     8701 ttgaacgctg ccgtcgtcat attgacgcct ataaagcccc tacgccagta gacgtcattg
     8761 aaggtgatat tcgcgatatc gccattgaaa acgcatcgat ggtggtgctg aattttaccc
     8821 tgcaattcct ggaaccttcc gagcgccagg cattactgga taaaatttat caaggactga
     8881 acccgggcgg cgcgctggtg ctttcggaaa aattcagttt cgaagatgcc aaagttggtg
     8941 aactgttgtt caacatgcac cacgacttta aacgtgccaa cggttacagc gaactggaga
     9001 tcagccagaa gcgcagcatg ctggaaaacg tgatgctgac cgattccgtg gaaacccata
     9061 aagcacgcct gcataaagcc ggttttgagc atagtgagct gtggttccag tgctttaact
     9121 ttggttcact ggtggcatta aaagcagagg acgctgcatg atcgactttg gtaactttta
     9181 ttctctgatt gccaaaaatc atctttcaca ctggctcgaa acgctgcccg cgcagattgc
     9241 taactggcag cgcgagcagc agcacgggtt gtttaagcag tggtcaaatg cggtggagtt
     9301 tctacctgaa attaaaccgt atcgtcttga tttattgcat agcgttacgg cagaaagcga
     9361 agagccactg agcgcagggc aaattaaacg cattgaaacg ctgatgcgca acctgatgcc
     9421 gtggcgcaaa gggccgttct cactgtatgg cgtcaacatc gataccgaat ggcgttccga
     9481 ctggaaatgg gatcgtgttc tgccccatct ttctgattta accggacgca ccattctgga
     9541 tgtcggctgc ggcagcggtt atcacatgtg gcgcatgatt ggcgcaggcg cgcacctcgc
     9601 ggtggggatc gaccctacgc aactttttct gtgccagttt gaagcggtgc gtaaactact
     9661 gggtaatgat cagcgcgcgc atttgttacc gttaggtatt gaacaacttc cggcactgaa
     9721 agcctttgat accgtctttt cgatgggcgt gctttaccat cgccgttcac cgctggagca
     9781 tctctggcag ttgaaagatc aactggtcaa tgaaggtgaa ctggtgctgg aaacgctggt
     9841 tattgatggt gatgagaaca cggtgctggt accaggcgat cgttacgcgc aaatgcgtaa
     9901 tgtctatttc atcccttccg cgctggcgct gaaaaactgg ctgaagaagt gtggttttgt
     9961 agatatccgc attgcagatg tgagcgttac cactacagaa gagcaacgac gcaccgaatg
    10021 gatggttact gaatcgctgg ccgatttcct cgacccacat gatccgagta agacagttga
    10081 aggttatccg gcacctaaac gtgcggtgct gattgcgcgc aagccgtaaa ggtctggtat
    10141 tactgccgga tgcggcgtga acgc
//
LOCUS       DACAPB010000162        10162 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.162, whole genome shotgun sequence.
ACCESSION   DACAPB010000162 DACAPB010000000
VERSION     DACAPB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.162"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..553
                     /locus_tag="IT904_003665"
     CDS             <1..553
                     /locus_tag="IT904_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193394.1"
                     /translation="VQFNDFPGFYDWMDYPDHPVKFVFHILENQKGTVWVYDQDDAFL
                     DIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEHHMPLLRIFPKGKECMHVEKWLRE
                     MSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDDARLNEWIS
                     NRVQELGDRYYPVNKHVKIRYTL"
     gene            966..1211
                     /locus_tag="IT904_003666"
     CDS             966..1211
                     /locus_tag="IT904_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193395.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
     gene            complement(1228..1899)
                     /locus_tag="IT904_003667"
     CDS             complement(1228..1899)
                     /locus_tag="IT904_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAP3193396.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2046..2321
                     /locus_tag="IT904_003668"
     CDS             2046..2321
                     /locus_tag="IT904_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP3193397.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2419..4005)
                     /gene="prpR"
                     /locus_tag="IT904_003669"
     CDS             complement(2419..4005)
                     /gene="prpR"
                     /locus_tag="IT904_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HAP3193398.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLTVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            4244..5134
                     /gene="prpB"
                     /locus_tag="IT904_003670"
     CDS             4244..5134
                     /gene="prpB"
                     /locus_tag="IT904_003670"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HAP3193399.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDNLFARSQVK"
     gene            5481..6650
                     /gene="prpC"
                     /locus_tag="IT904_003671"
     CDS             5481..6650
                     /gene="prpC"
                     /locus_tag="IT904_003671"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HAP3193400.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            6684..8135
                     /gene="prpD"
                     /locus_tag="IT904_003672"
     CDS             6684..8135
                     /gene="prpD"
                     /locus_tag="IT904_003672"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HAP3193401.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8175..10061
                     /gene="prpE"
                     /locus_tag="IT904_003673"
     CDS             8175..10061
                     /gene="prpE"
                     /locus_tag="IT904_003673"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HAP3193402.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2464 a         2639 c         2745 g         2314 t
ORIGIN      
        1 tgtgcagttt aatgatttcc ctggatttta tgactggatg gattaccctg atcacccggt
       61 taagtttgtt tttcacatac ttgaaaatca aaaagggaca gtttgggtat atgaccagga
      121 tgatgcgttt cttgatatta aagcaaacgt gcaggctgga cgctttaccg ggcttaagaa
      181 attagtgcag tttatcgatt cagtgcgaac agattgcaaa tgtattcttc ttgaacacca
      241 tatgcctttg ctgagaattt tccctaaagg gaaagagtgt atgcatgtgg aaaaatggct
      301 tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt aagcaggcgc ttgcgcatgg
      361 attattatta cacttaaaaa atatctaccc tgtttttcct gaatcactgg tgatgttatt
      421 gctctctgtt ctggacgtaa aaacatacag agatgatgcg cgattaaatg aatggatatc
      481 taatagagtg caggaattag gtgacagata ttatcctgta aataagcacg ttaaaatccg
      541 ctacactctt tagttcagaa agggttccac ctgcaaattg ttattccacc tgagagtgaa
      601 tcgcaatgca ggtaatcatt tcatctgtat atctgtatgc caacaggcat tccatctctt
      661 ttttcgccta tattttgttg tgatcggcga cacttcgctt aaaaaagcac cagtagtggt
      721 ttcgcagcca tgcggtgtat aaaaaatgat ctcatgcgga tgttttgtga atgtgtgggt
      781 tgacattcat atgaaaaaaa tcataattcc atcatgtttg tgtggggtct tttctgtatc
      841 ttacgcatcg cactcaagcc tgacagaaaa tatgctgtaa ggctcatatc aaaaaccgcc
      901 attagctcat caggaagagc agacgacaac caggattgtt gtatggtacg gggttcgagg
      961 cctcgatggc ggtccaactt ttcaaaactc ttctgaatca gatcccctta ttatcatccc
     1021 tgcaaagtgg aacattgcca ttgtttggct attcaggatg gggcagacca atgaaaaaag
     1081 cccacacagg ggagagtggg ctgaaatggg aagctaaaga ctcaagtaaa cttatcggaa
     1141 ataaggacca cgcattacgg gggctatcat cgccgatggc cgtgattcgg caaatcaggt
     1201 tgattacgta gagagtaaat tatctgctca ccgctgcgtc accccttcgt aaatcaggcg
     1261 tagcgcgaat acaccaataa ttgcaccaat aacccgactg gcaacgcgtt gcatacgccc
     1321 ataagcacga cgcacagcgg gcaaagaaaa cgcctgacta agaaaaactc gccagataat
     1381 tgatgcgagc acaatccccg cccaggccat taaacgtgcc catgttggtg tttcggcatt
     1441 taatgttact gagaaaatac tgataaaaaa taaaacggtt tgcgggttag agagatcggt
     1501 aattaatccg cggcgaaaaa agacatacca gggggcgcta atcggttgtt gtagtgtgct
     1561 catttgcggt gttgactggc ggcgcatgct gcaccacgca aaccataaga gataagcgcc
     1621 gccgacgatt ctgataagcg aaaaaatctc ctcacactgc gtaattagcg ttgcaagacc
     1681 aaacaaaccc aacccggaat aaaatgcatc gcccagcgcc acgcccagcc cggtcagcac
     1741 ccctgcgcgt cgaccggaag ccaggctggt ttgtactacc acaaagagat tggctcccgg
     1801 attaaaaaaa gtaatcacga acagtcctac ggtcaggtaa acggcatgca aaggatccat
     1861 agtgatttca tccataaata agtgaactaa ctgcatcata tttctaccaa aaataatcgg
     1921 gtgcgagaga gatcacaaag tgtcttattt ccggttactg gcgtttatgc cctgactgaa
     1981 ctaattatta atcaacccaa taatgtcggt gggtgatagt gtgataacaa ctctggagcc
     2041 gtaatatgaa aataatctct aaaatgttag tcggtgcgtt agcgtttgcc gttaccaatg
     2101 tctatgccgc tgaattgatg actaaagcgg aatttgaaaa agtcgaatcg caatatgaaa
     2161 aaataggtga tatttcaacc agcaatgaaa tgtcgaccgc agatgcgaaa gaggatttga
     2221 tcaaaaaagc ggatgaaaaa ggggctgatg tgttggtact gacctccggt caaactgaca
     2281 ataagatcca cggcacggca aatatttata agaagaagta attctgaatc ctatgtaaac
     2341 atccccgatg cgtaagttta tcggtgatca tctattgaaa tttatgccgg ataaagcgtt
     2401 cgcgctgcat tcggcagttc agtttttcag ccgccgccag aacgtcgtcc ggctgatgcc
     2461 taaataattc gccgctgctg ttttatcgcc attaaatttc tccagtgcct gttgtggtgt
     2521 cagtaagcgt ggagcgggag ttttcgccga ctcgcgcgcc agttccggca gtagcagctg
     2581 caaaaattgc ggcgttaaat ccggcgtcgg ttccacactt aaaaatagcg ccagtcgctc
     2641 catcatattg cgcagttcac gaatattgcc cggccagtcg tagtgcacca gcacggtttc
     2701 gcttgcctgt aatccctggc gtaatgcggc agaaaacggg gcggagagcg ccgccagaga
     2761 cactgtcaaa aagctttccg ccagtggcag aatatccgcc acccgctcgc gcagtggtgg
     2821 caattgcaga cgcaaaatac tcagccgata aaacaggtca cggcgaaact gcccttgccg
     2881 catatcttct tccagattgc agtgagtggc gctaatgacc cgcacatcca ccggaacagg
     2941 ctgatgcccg ccgacgcggg tgacctcttt ttcttccagc acccgcagca gccgggtctg
     3001 caacggcagc ggcatttcgc caatctcatc gagaaacagc gtacctccgt gggcgatttc
     3061 aaacagcccg gcgcgaccgc cgcgtcgcga gccggtaaac gccccttcct catagccaaa
     3121 cagttctgct tccagcagcg attcggcaat cgccccgcag ttgactgcaa caaacggatg
     3181 cgactttttg ccctgtcgcg catcgtggcg ggcaaaatat tcccgatgaa tcgcctgggc
     3241 cgccagctct ttgcccgtcc ccgtttcccc ctcaatcaac accgctgcac tggagcgggc
     3301 atacagcaaa atagtctgcc gtacttgttc catctgtggt gattgaccga gcatatcgcc
     3361 cagcacgtaa cgagttctca gggcgttgcg ggtggcatcg tgagtgttat ggcgtaacga
     3421 catgcgcgtc atatccagcg catcgctgaa cgcctggcgc acggtggcgg cggaatagat
     3481 aaaaattccg gtcattccgg cttcttctgc cagatcggta atcagccccg cgccgaccac
     3541 cgcttcggtg ccgttagctt ttagctcgtt aatctgcccg cgtgcgtctt cttcggtaat
     3601 gtagctacgt tgatcgaggc gcaaattaaa ggttttttga aacgccacca gtgccggaat
     3661 agtttcctga taagtgacaa cgccgataga agaggtgagt tttccggctt ttgccagtgc
     3721 ctgtaacaca tcgtagccgc tcggtttaat caaaataact ggcactgaca ggcggctttt
     3781 caggtacgcg ccgttagatc cagcggcgat gatggcgtca cagcgttcgt ttgccagttt
     3841 cttgtggatg taggtcaccg ctttttcaaa gccaagctgg ataggggtaa tgttcgccag
     3901 gtgatcaaac tcgaggctga tatcgcgaaa cagctcgaac aggcgcgtta cagataccgt
     3961 ccagataacc ggtttgtcgt cattaagccg tggtggatgt gccatagcgc accgcaaagt
     4021 taagaaaccg aatattgggt ttagtcttgt ttcataattg ttgcaatgaa acgcggtgaa
     4081 acattgcctg aaacgttaac tgaaacgcat atttgcggat tagttcatga ctttatctct
     4141 aacaaattga aattaaacat ttaattttat taaggcaatt gtggcacacc ccttgctttg
     4201 tctttatcaa cgcaaataac aagttgataa caaaggatgg gctatgtctc tacactctcc
     4261 aggtaaagcg tttcgcgctg cactgactaa agaaaatcca ttgcagattg ttggcaccat
     4321 caacgctaat catgcgctgt tggcgcagcg tgccggatat caggcaattt atctttctgg
     4381 cggtggcgtg gcggcaggtt cgctggggct gcccgatctc ggtatttcta ctcttgatga
     4441 cgtgctgacc gatattcgcc gtatcaccga cgtttgttcg ctgccgctgc tggtggatgc
     4501 ggatatcggt tttggttctt cggcctttaa cgtggcgcgc accgtgaaat cgatgattaa
     4561 agccggtgcg gcaggattgc atattgaaga tcaggttggt gcgaaacgct gcggtcatcg
     4621 tccgaataaa gcgatcgtct cgaaagaaga gatggtggat cggatccgcg cggcggtgga
     4681 tgcgaaaacc gatcctgatt ttgtgatcat ggcgcgcacc gatgctctgg cggtagaggg
     4741 gctggatgcg gcgatcgagc gtgcgcaggc ctatgttgaa gcgggtgccg agatgttgtt
     4801 cccggaggcg attaccgaac tcgccatgta ccgccagttt gccgatgcgg tgcaggtgcc
     4861 gatcctcgcc aacatcaccg aatttggtgc cacgccgctg tttaccaccg acgaattacg
     4921 cagcgcccat gtcgcaatgg cgctgtaccc actttcagcg ttccgcgcca tgaaccgcgc
     4981 cgctgaacat gtctacaacg tcctgcgcca ggaaggcacg cagaaaagcg tcatcgacac
     5041 catgcagacc cgcaacgagc tgtacgaaag catcaactac taccagtacg aagagaagct
     5101 cgacaacctg tttgcccgta gccaggtgaa ataaaaaacg cccgttgatt gtattcgaca
     5161 gccgatgcct gatgcgtcgc tgacgcgact tatcaggcct acgaggtgca ctgaactgta
     5221 ggtcggataa gacggatggc gtcgcatccg acaaccgatg cctgatgcgc cgctgacgtg
     5281 acttatcagg cctacggggt gcactgaact gtaggtcgga taagacgcat agcgtcgcat
     5341 ccgacaaccg atgcctgatg cgccgctgac gcgacttatc aggcctacgg ggtgaactga
     5401 actgtagatc ggataagacg catagcgtcg catccgacaa caatctcgac cctacaaatg
     5461 ataacaatga cgaggacaac atgagcgaca caacgatcct gcaaaacagt acccatgtca
     5521 ttaaaccgaa aaaatctgtg gcactttctg gcgttccggc gggcaatacg gcgctctgca
     5581 ccgtgggtaa aagtggcaat gacctgcatt accgcggcta cgatattctt gatctggcga
     5641 aacattgcga atttgaagaa gtggcgcatc tgctgatcca cggcaaactg ccgacccgtg
     5701 acgaactcgc cgcttacaaa acgaaactga aagccctgcg cggtttaccg gctaacgtgc
     5761 gtaccgtgct ggaagcctta ccggcggcgt cgcacccgat ggatgttatg cgcaccggtg
     5821 tttccgcgct cggttgcacg ctgccagaaa aagaggggca taccgtctct ggcgcgcggg
     5881 atattgccga caaactgctg gcgtcgctta gctcgattct cctttattgg tatcactaca
     5941 gccacaacgg cgaacgcatc caaccggaaa ccgatgacga ctccatcggc ggtcacttcc
     6001 tgcatctgct gcacggcgaa aagccatcgc aaagctggga aaaggcgatg catatctcgc
     6061 tggtgctgta cgccgaacac gagtttaacg cctccacctt taccagtcgg gtgattgcgg
     6121 gcaccggctc tgatatgtat tccgcgatta ttggcgcgat tggcgcactg cgcgggccaa
     6181 aacacggcgg ggcgaatgaa gtgtcgctgg agatccagca acgctacgaa acgccggacg
     6241 aagccgaagc agatatccgc aagcgcgtgg aaaacaaaga agtggtcatt ggttttggtc
     6301 atccggttta caccatcgct gacccgcgcc accaggtgat taaacgtgtg gcgaagcagc
     6361 tctcgcagga aggcggctcg ctgaagatgt acaacatcgc cgatcgcctg gaaacggtga
     6421 tgtgggagag caaaaagatg ttccccaatc tcgactggtt ctctgctgtt tcctacaaca
     6481 tgatgggcgt tcccaccgag atgttcacac cactgtttgt tatcgcccgc gtcaccggct
     6541 gggcggcgca cattatcgaa caacgtcagg acaacaaaat tatccgtcct tccgccaatt
     6601 atgttggacc ggaagaccgc ccgtttgtcg cgctggataa gcgccagtaa acctctacga
     6661 ataacaataa ggaaacgtac ccaatgtcag ctcaaatcaa caacatccgc ccggaatttg
     6721 atcgtgaaat cgttgatatc gtcgattacg tcatgaacta cgaaatcagc tctaaagtgg
     6781 cctacgacac cgcacattac tgcctgctcg acacgctcgg ctgcggtctg gaagctctcg
     6841 aatacccggc ctgtaaaaaa ctgctggggc caattgttcc cggcaccgtc gtacccaacg
     6901 gcgtgcgcgt ccccggaact cagttccagc tcgaccccgt ccaggcggca tttaacatcg
     6961 gcgcgatgat ccgctggctc gatttcaacg atacctggct ggcggcggag tggggccatc
     7021 cttccgacaa cctcggcggc attctggcaa cggcggactg gctttcgcgc aacgcggtcg
     7081 ccagcggcaa agcgccgttg accatgaaac aggtgctgac cgcaatgatc aaagcccatg
     7141 aaattcaggg ctgcatcgcg ctggaaaact cctttaaccg cgtcggcctc gaccacgttc
     7201 tgttagtgaa agtggcttcc accgccgtgg tcgccgaaat gctcggcctg acccgcgagg
     7261 aaattctcaa cgccgtttcg ctggcgtggg tggacggtca gtcgctgcgc acctatcgcc
     7321 atgcgccgaa caccggcacg cgtaaatcct gggcggcggg cgatgccact tcccgcgcgg
     7381 tacgtctggc actgatggcg aaaacgggcg aaatgggtta cccgtcagcc ctgactgcgc
     7441 cggtgtgggg cttctacgac gtctccttta aaggtgaatc gttccgcttc cagcgcccgt
     7501 acggttccta cgttatggaa aatgtgctgt tcaaaatctc cttcccggcg gagttccact
     7561 cccagacggc agttgaagca gcgatgacgc tctatgaaca gatgcaggca gcaggcaaaa
     7621 cggcggcgga tatcgaaaaa gtgaccattc gcacccacga agcctgtatt cgcatcatcg
     7681 acaaaaaagg gccgctcaat aacccggcag accgcgatca ctgcattcag tacatggtgg
     7741 cgatcccgct gctattcggg cgcttaacgg cggcagatta cgaggacaac gttgcgcaag
     7801 ataaacgcat tgacgccctg cgcgagaaga tcaattgctt tgaagatccg gcatttaccg
     7861 ctgactacca cgacccggaa aaacgcgcca tcgccaatgc cattaccctt gagttcaccg
     7921 acggcacacg atttgaagaa gtggtggtgg agtaccccat tggtcatgct cgccgccgtc
     7981 aggatggtat tccgaaactg gtcgataaat tcaaaatcaa tctcgcgcgc cagttcccga
     8041 ctcgccagca gcagcgcatt ctggaggttt ctctcgacag agctcgcctg gaacagatgc
     8101 cggtcaatga gtatctcgac ctgtacgtca tttaagtaaa cggcggtaag gcgtaagttc
     8161 aacaggagag cattatgtct tttagcgaat tttatcagcg ttcgattaac gaaccggagc
     8221 agttctgggc cgagcaggcc cggcgtattg actggcagac gccctttacg caaacgctcg
     8281 atcacagcaa tccgccgttt gcccgttggt tttgtgaagg ccgaaccaac ttgtgccaca
     8341 acgccatcga ccgctggctg gagaaacagc cagaggcgct ggcgctgatt gccgtctctt
     8401 cggaaacaga agaagagcgc acctttacct ttcgtcagct gcatgacgaa gtgaacgcgg
     8461 tggcctcaat gttgcgttca ttgggtgtgc agcgcggcga tcgggtgctg gtgtatatgc
     8521 cgatgattgc cgaagcgcat attactctgc tggcctgcgc gcgcattggc gctattcact
     8581 cggtggtgtt tggtggattt gcctcgcaca gcgtggcggc gcgaattgat gacgctaaac
     8641 cggtgctgat tgtctcggct gatgccggag cgcgcggtgg caaaatcatt ccctataaaa
     8701 aattgctcga cgatgcgata agtcaggcgc agcaccagcc acgccatgtt ttgctggtgg
     8761 atcgcgggct ggcgaaaatg gcgcgcgtca gcgggcggga tgtcgatttc gcgtcgttgc
     8821 gccatcaaca catcggcgcg cgggtaccgg tggcgtggct ggaatccaac gaaacctcct
     8881 gcattctcta cacttccggc acgaccggca aacctaaagg cgtgcagcgt gacgtcggcg
     8941 gatatgcggt ggcgctggcg acctcgatgg acaccatttt tggcggcaaa gcgggcagcg
     9001 tgttcttttg cgcatcggat atcggctggg tggtggggca ttcgtatatc gtttacgcgc
     9061 cgctgctggc ggggatggcg actatcgttt acgaaggatt gccaacctgg ccggactgcg
     9121 gcgtgtggtg gaaaatcgtc gagaaatatc aggttagccg gatgttctca gcgccgaccg
     9181 ccattcgcgt gctgaaaaaa ttccctaccg ctgaaattcg caaacacgat ctctcgtcgc
     9241 tggaagtgct ctatctggct ggagaaccgc tggacgagcc gaccgccagt tgggtgagca
     9301 atacgctgga tgtgccggtc atcgacaact actggcagac cgaatccggc tggccgatta
     9361 tggcgattgc tcgcggtctg gacgacaggc cgacgcgtct gggaagcccc ggtgtgccga
     9421 tgtatggcta taacgtgcag ttgcttaatg aagtcaccgg cgaaccgtgt ggcgtcaacg
     9481 agaaagggat gctggtggtg gaagggccgc tgccgccggg gtgtattcag accatctggg
     9541 gcgacgacgg ccgctttgtg aagacttact ggtcgctgtt ttcccgcccg gtgtacgcca
     9601 cctttgactg gggcatccgt gacgctgacg gttatcactt tattctcggg cgcactgacg
     9661 atgtaattaa cgttgccggg catcggctgg ggacgcgcga gattgaagag agtatctcca
     9721 gccatccggg cgttgccgaa gtggcggtgg ttggggtgaa agatgcgctg aaagggcagg
     9781 tggcggtggc gtttgtcatt ccgaaagaga gcgacagtct ggaagatcgt gatgtggcgc
     9841 actcgcaaga gaaggcgatt atggcgctgg tggacagcca gattggcaac tttggccgcc
     9901 cggcgcacgt ctggtttgtc tcgcaattgc caaaaacgcg atccggaaaa atgctgcgcc
     9961 gcacgatcca ggcgatttgc gaaggacgcg atcctggaga tctgacgacc attgatgatc
    10021 ctgcgtcgtt ggatcagatc cgccaggcga tggaagagta ggttattgtc ggatgcgtcg
    10081 cgcggtgcat ccggcactgt gtgccgatgc ctgatgcgac gctgacgcgt cttatcatgc
    10141 ctacggacct gaaccgtagg tc
//
LOCUS       DACAPB010000163        10155 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.163, whole genome shotgun sequence.
ACCESSION   DACAPB010000163 DACAPB010000000
VERSION     DACAPB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.163"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..770)
                     /locus_tag="IT904_003674"
     CDS             complement(<1..770)
                     /locus_tag="IT904_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HAP3193403.1"
                     /translation="MTTKNMNTLPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQ"
     gene            complement(790..1236)
                     /gene="etp"
                     /locus_tag="IT904_003675"
                     /gene_synonym="yccY"
     CDS             complement(790..1236)
                     /gene="etp"
                     /locus_tag="IT904_003675"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAP3193404.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(1224..2363)
                     /locus_tag="IT904_003676"
     CDS             complement(1224..2363)
                     /locus_tag="IT904_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAP3193405.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(2409..4505)
                     /locus_tag="IT904_003677"
     CDS             complement(2409..4505)
                     /locus_tag="IT904_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAP3193406.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIACTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(4505..5251)
                     /locus_tag="IT904_003678"
     CDS             complement(4505..5251)
                     /locus_tag="IT904_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAP3193407.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(5248..5880)
                     /locus_tag="IT904_003679"
     CDS             complement(5248..5880)
                     /locus_tag="IT904_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAP3193408.1"
                     /translation="MLSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQIQALP
                     YSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLLEVTN
                     SGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNVLCNV
                     WQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(5999..6304)
                     /locus_tag="IT904_003680"
     CDS             complement(5999..6304)
                     /locus_tag="IT904_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HAP3193409.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(6746..6958)
                     /gene="cspH"
                     /locus_tag="IT904_003681"
     CDS             complement(6746..6958)
                     /gene="cspH"
                     /locus_tag="IT904_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAP3193410.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            7244..7456
                     /gene="cspG"
                     /locus_tag="IT904_003682"
     CDS             7244..7456
                     /gene="cspG"
                     /locus_tag="IT904_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAP3193411.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            7467..7655
                     /locus_tag="IT904_003683"
     CDS             7467..7655
                     /locus_tag="IT904_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP3193412.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            7630..7860
                     /gene="ymcE"
                     /locus_tag="IT904_003684"
     CDS             7630..7860
                     /gene="ymcE"
                     /locus_tag="IT904_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAP3193413.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            7850..8023
                     /locus_tag="IT904_003685"
     CDS             7850..8023
                     /locus_tag="IT904_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAP3193414.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(8071..9144)
                     /locus_tag="IT904_003686"
     CDS             complement(8071..9144)
                     /locus_tag="IT904_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAP3193415.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(9216..>10155)
                     /locus_tag="IT904_003687"
     CDS             complement(9216..>10155)
                     /locus_tag="IT904_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP3193416.1"
                     /translation="GIDPAKLAEIFQPFVQVSGKRGGTGLGLTISSRLAQAMGGELSA
                     TSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGLRLLLIEDNPLTQRITIEMLKTS
                     GAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDIDGITLARQLAQQYPSLVLIGFSA
                     HVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYLQLQVNNDQSLDVSQLNEDAQLM
                     GTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIKRAAHQLKSSCSSLGMHIASQLC
                     AQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI"
BASE COUNT         2571 a         2543 c         2439 g         2602 t
ORIGIN      
        1 ttctgttgca aatagttgtt agcgatgctg ttcagaatac gagtaatcaa ctggggatca
       61 tcaccagtca tggtaagttc cagcatcccg ctttctttac tgcgttcgct aacggtaaag
      121 gtttcctgca atgcgttaat cgcttccagt tcggtacgct ggctcaggac aaactgtgtt
      181 cctggtttgg ccttaatgtc cgcgatagtc agcgcaacgc catctttttc cagacgctgg
      241 ccgaccatac cattgacggt gaactcttca ccttccagcg tatagtggcc gttttcccca
      301 accgtgagtg tcagttgttg atcctgacca ttcagttgtg gaatatgcat ccagctgatc
      361 gccagctcac ctggtttctc tttggttaat ctcgcccagc cgcgacccac aatcggaaaa
      421 tacttctgct caaccatgtc gcgcagattc agttcagcaa tggttttacc gagaatcatg
      481 cgcgattgca gcagttggat ctccggtgca gactcgggcg atgagttagg gatcatatcg
      541 ctcaggccac tgagaatggc gttgccctgt ttctgctcaa cctggaccag agtatctgcc
      601 tgataaattg gcgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa taacgcggtc
      661 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
      721 tcattttcct gagtgctgcc tggtagcgta ttcatatttt tagttgtcat cgtatcttat
      781 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatacc
      841 gtagacatgt tcaaatgcgt cctgactttt acgataggga tcagggatct ctttctgttc
      901 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcgattg cggtaacctg
      961 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
     1021 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgctgc
     1081 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
     1141 tggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
     1201 aaccaccagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
     1261 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
     1321 gccagcggcg cagtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
     1381 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
     1441 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
     1501 ataccctcag cgttacccag cgcttccgcc agggtcatac cgctgcggtc cattttcagc
     1561 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
     1621 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
     1681 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
     1741 gcatctgcgg tcagcccccc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
     1801 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgagcgg
     1861 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatccag acgcgctgta
     1921 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
     1981 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
     2041 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atccccaatg
     2101 ccgatacgat attcatagtt cgcgatttgc tgctccagct ccgggttagc ctgcgacata
     2161 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
     2221 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
     2281 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
     2341 aacttaaaaa tgttcttttt cattggttta tatttatcag tcattgtaat gttatgaatt
     2401 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata gagctggtat
     2461 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
     2521 ttgcggttag gtccaatggt catcaagtcg aatggaatcg agatataaaa acctttacta
     2581 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
     2641 gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
     2701 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
     2761 gtcacgaaac ctgttggcgt ggaataatcg gtgaaacgca tcatgttgtc ccagtcacgc
     2821 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaagc
     2881 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
     2941 aaaccattgc ctaagtcagc aaaatagttc gcctgcaagt tgttgagata aacgtcattg
     3001 cgaacgtagt cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggcaacagc
     3061 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgcc atcaagcagc
     3121 aggtggtcgg taaaccagta gcgggcactg gacatcagcc ccagctggaa catatagaaa
     3181 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
     3241 tcacgaatac ggtagccccg accaaaggca gaaagatctt cagcttcaac acgttgttgt
     3301 tgcagtggct ctgattgacc aggcgctgta cctgccagct gtttgcgcag gctggctacg
     3361 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
     3421 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcacgagag
     3481 tcacggtatt tgtattgctg accagacata tacagtgtct tatcgcgtag ctgaatttct
     3541 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
     3601 tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc gcgcagggca
     3661 gggcgcagat cgttgaaatt ggtccgtaac gtgaagccaa acatcaacgt gttaccgcgt
     3721 tcataactca ggttgagatc tgcccagctg gcagcgcgat aaactgcgcc gacgttgaaa
     3781 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgtttcc atcgtattcg
     3841 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
     3901 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
     3961 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
     4021 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatac
     4081 tcaccgtcaa acagacccgt acaagcaata tcacgtttac caaacgccac ttgcggtagc
     4141 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
     4201 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
     4261 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
     4321 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
     4381 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
     4441 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
     4501 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
     4561 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
     4621 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
     4681 tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc agataatcgg
     4741 taacgctacg gcctgcctgc cagggcaatt gcccggcccc agaaaccgca cccagcagag
     4801 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
     4861 tgttctcatc cactcgtaca aaatcaggat ccagtttgac cggtaagcga ccagtgatat
     4921 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
     4981 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
     5041 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
     5101 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgccag
     5161 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
     5221 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
     5281 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
     5341 gacccagaat gtgttttgcc actgtttttc tggccgcgtg gaagtcacct cttcctgcca
     5401 gacattacac agcacattgc gacccgcaat attcagcgtc tctaatccag caaaggaaaa
     5461 agtagaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
     5521 ccagcggctg ccatctttta ttgccagcgc gttacgcaga gggtcctgcc cggaatttgt
     5581 cacttccagc agattattat taagtttgac ggtttttaaa agacgtccat tgtgggtaac
     5641 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
     5701 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
     5761 gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
     5821 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
     5881 aagagggcgt acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
     5941 ggtacgaata tcgcaaccgg gtatcattta actttaattt cgacgcgtta aagcagtatt
     6001 agcgtgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
     6061 ctgcaacaac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
     6121 tgccgacagc gccagcagcc acgccaccag tggtagtagc agtcgtggtt gcgccggtag
     6181 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgtt gcttcagggg
     6241 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
     6301 tcataacttt tcttttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
     6361 actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc actatatact
     6421 gcgggtagga aaggcgcgca gagggaaata taagattgtt aactatcccc ctctggaaat
     6481 acgatttgtg agcataagat aaatcttagc aaaatacaaa ttaaagataa ggaaattgtt
     6541 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
     6601 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttctttgtg
     6661 gaaaagtaac tgatgttata atgtatgatg ttgactattg cgctattcca ataaaaccag
     6721 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
     6781 gcaggccatt tacgcggcaa aattccacac gtaatcctgg tataagcact tctgcgtcgc
     6841 ggggagtgaa tgcggaaata tggacctgaa cttctttacg accgtcggag gggataatga
     6901 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
     6961 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
     7021 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgcgttaa
     7081 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
     7141 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag tttctgtatt
     7201 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaaatgac
     7261 tggtttagta aaatggttta acgcagataa aggttttggc tttatcactc ctgatgatgg
     7321 cagcaaagac gttttcgtcc atttcactgc catccagagc aatgaattcc gcacgctgaa
     7381 cgaaaatcag aaagttgaat tttctattga gcaggggcaa cgtggccccg cggcagcgaa
     7441 cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc tgtccatgtt
     7501 gtcatggttc acagtaccgt acatcggcat tcgatgtgac ggagcgaaac cctttgggcg
     7561 ctaagtgtat tttttgtaaa tcgacgatga tcacctttga taacgtcgcg ctgcaaatac
     7621 gcacggacca tgcgccgctg gatttcacaa aataatatca ggctccctcg tggagccttt
     7681 tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa cctgttgatt
     7741 attgaatctt tcggggagat ggcttataac atttcttacc tgaccagggt accgggaacc
     7801 aacaccttac tggcgtgttg ctgtctttta agaccagaag aggttaacag tgaatattga
     7861 agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa aaatcgccga
     7921 gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcaccgcac gagaaaaaat
     7981 gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg aacatcaccc
     8041 gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg cttgtggaat
     8101 taagcgtcgg tacaattcat ccggtaccgg gctttgccat actcccgcat acattgcgta
     8161 accaatcacc gcaaacataa tccccagaac cagtagcgtc attaaccagc cagacaacgc
     8221 aaaggctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg aggctaccgg
     8281 acatgactct acgcaagtca tacagccggt acattccact gttcgtacct gaattaattt
     8341 atcgaccggg atccgtgatg ggcaattttt tgcgcatttg ccacagtcga tacaactttc
     8401 ggcattgcga cgaatcttaa acggcgacaa tagcgaaacc acgcccatca gcgcgccata
     8461 tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca gcaaaacggt
     8521 cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt taacgtccat
     8581 caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca tcaataacgc
     8641 gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa tatccagcca
     8701 gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga ttaattcaga
     8761 aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata gactgatgac
     8821 gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact gacctgttac
     8881 aaggctatat ttcagattca tcagcccggc aatcggtagc cagccttcga tacctcccgg
     8941 tctggcgaca aatgtcgtgc tacttgccgt ttcgtaatag cgcacccaat accagaacgt
     9001 gatggcaata taaatattca ttgccaacag taataattgc gtcgctttac gccaggtcgt
     9061 ggcattacgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc gccgctgcca
     9121 gcgggttctt ttattctctg ccatgatttt gccagtccgt taagttgtat accaaatgcc
     9181 actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag gtctttctta
     9241 tgcaaccatg cttccagagc ggcaacactg cgtgtaattt cttcgtgtgg aaggggggca
     9301 gataatggct gctgctccag ttgtgcgcat agctggctgg cgatatgcat tcccagactc
     9361 gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctatc ctgactggcg
     9421 cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtgtaaataa taccagccat
     9481 tcgtggatct tctccgtccc cattaactga gcatcttcat tgagttgcga tacatccagc
     9541 gattgatcat tattgacttg cagttggaga tagtgcgcca gtaactgacc gagcacttca
     9601 cgcggcaccg gtttagggat aatcccgcgg aataatgaac tggtacgctg gcgcagcgtt
     9661 tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg ctgtgccagt
     9721 tgtcgggcaa gcgtaatgcc gtcgatatcc ggcagatcaa aatccaccag tgcggcagca
     9781 aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaac agcaacaatc
     9841 tgcgcaccac tggttttcag catctcaatg gtaattcgct gggttagcgg gttatcttca
     9901 attaacagca aacgtaaacc gtcaagacgc accgcctgat tgactgtttt tggcacgggt
     9961 gccgtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc cggcgtgctg
    10021 gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt cagtcccagc
    10081 ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc tgccagtttc
    10141 gcgggatcaa taccg
//
LOCUS       DACAPB010000164         9969 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.164, whole genome shotgun sequence.
ACCESSION   DACAPB010000164 DACAPB010000000
VERSION     DACAPB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.164"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(129..1229)
                     /gene="chaA"
                     /locus_tag="IT904_003688"
     CDS             complement(129..1229)
                     /gene="chaA"
                     /locus_tag="IT904_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAP3193417.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1499..1729
                     /gene="chaB"
                     /locus_tag="IT904_003689"
     CDS             1499..1729
                     /gene="chaB"
                     /locus_tag="IT904_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAP3193418.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1887..2582
                     /locus_tag="IT904_003690"
     CDS             1887..2582
                     /locus_tag="IT904_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP3193419.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2626..2979)
                     /gene="ychN"
                     /locus_tag="IT904_003691"
     CDS             complement(2626..2979)
                     /gene="ychN"
                     /locus_tag="IT904_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAP3193420.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3165..4559
                     /gene="ychO"
                     /locus_tag="IT904_003692"
     CDS             3165..4559
                     /gene="ychO"
                     /locus_tag="IT904_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAP3193421.1"
                     /translation="MSRFVPRIISFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGEISMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4560..5210)
                     /gene="narL"
                     /locus_tag="IT904_003693"
     CDS             complement(4560..5210)
                     /gene="narL"
                     /locus_tag="IT904_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAP3193422.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5203..6999)
                     /gene="narX"
                     /locus_tag="IT904_003694"
     CDS             complement(5203..6999)
                     /gene="narX"
                     /locus_tag="IT904_003694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAP3193423.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAALPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7338..8729
                     /gene="narK"
                     /locus_tag="IT904_003695"
     CDS             7338..8729
                     /gene="narK"
                     /locus_tag="IT904_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAP3193424.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9245..>9969
                     /locus_tag="IT904_003696"
     CDS             9245..>9969
                     /locus_tag="IT904_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP3193425.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESA"
BASE COUNT         2436 a         2432 c         2555 g         2546 t
ORIGIN      
        1 gtttcgtgca ggttgtggta caggctcgca attctgatta acgacctgtc caggtagtat
       61 gaagaaatac aataagcagg aatttatatt atctcccccg atttccgtca tcggaggaga
      121 tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag atgcgctgcg
      181 ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac taaagaggcc
      241 accatcacca ccatttctgg cgcaccaagt gcaaactgta attcgttacc cgtcataaag
      301 gcaattagcg tgacgacagg tacggtaagc gaaatggttg ctaacactga accaaagaac
      361 agattcatcg cgcgctggac ctggttgttc aacactgctt ttaacgcacc taaaccttcc
      421 ggcgacagaa tcaacagagc caccaggaag ccagtaaagg cgacaggggc attcatgctg
      481 tcgagcaatg tctccagcgg gctggcattc attttggtga ctgcaataac ggcaatcaga
      541 tggataatca accagatagc atgccacacg ctgctatggg cagacggttt accgtgatgc
      601 gggtcgtcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg atgcgttttg
      661 gtctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac cagtaacgcc
      721 tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac gattatcgcc
      781 agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata ttgggtggca
      841 aacttacgac cgcccaacaa taatgaaaag ccaaccagcc caccggtaac aatcataatg
      901 attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc cattaaagct
      961 gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga accgtaaggt
     1021 tctcccaggc gatgggctaa tacgtccgca tgacggacaa cactaaaggc gctacttaaa
     1081 ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg gctgcttccc
     1141 cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaagtctc cttgtggcgg
     1201 gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat gaatttatcg
     1261 aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg taacatttta
     1321 cgaattttta tcccactgtc gattttttac tcattgggac ataaaaataa gtattacgtt
     1381 tagacaatgt ttgttttagc gtttcttaag aacagtctga catgaaaatt cttatgtttt
     1441 ggcaagagta gatattgttg accacactta atgttcaact ttgtaaaagg agtcaacgat
     1501 gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgttctac cgtctcatgc
     1561 ccaggatatc tataaagaag cgttcaacag cgcgtgggat caatataaag ataaagagga
     1621 tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg ctgctgtgaa
     1681 gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa accggtagct
     1741 tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc atattgtccc
     1801 gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga caccaaaaag ttgtcgcagg
     1861 gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg aatgccgatt
     1921 gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagag caacgggcgg
     1981 cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg atcttcggtt
     2041 atggatcgtt gatgtggaat ccggcactgg agtttaccga gtcgtgcacc ggtacactgg
     2101 ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg gcgcgggact gcgcaccagc
     2161 cgggacggat gcttgcactg aaagagggcg gacgcactac aggcgtcgcc tatcgactgc
     2221 cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg attaccggct
     2281 gttatctgcc aacctggtgt cagcttgatc ttgatgatgg atgcacagta aacgccattg
     2341 tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct caggtcatcg
     2401 cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa cgcacaatac ctgttttcac
     2461 tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat ttgctggtat
     2521 cggtaaaaaa actgctggcg gagaattatc cggatggtgt gttacggccg ggattcgcct
     2581 gagtaaactt ccctcatagt ggggcgtcag acgccccctc aaacattaaa atgtgagcac
     2641 tttatcggct gacagcgtcc attgcgccag ttccacaaga gtaccgattt ccaccccatc
     2701 aataagagga agtgtgctaa tcccgcgccc gtcggtacag gttttgcaca atttcaccgg
     2761 tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt cccctggttt
     2821 ttgcccgcgc aacccggctg tgaccgcatc agacatgagg aacagacgca gatccagatt
     2881 gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg attcgctccc
     2941 gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt atttactcct gtattcaggg
     3001 aattagacac tcatcttcta tcttactgct tctgcagcgt ctgaccaatc ggtcacattt
     3061 ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa tcgttattct
     3121 taaaggctat ggtttttcat tttttaccgg aagttaccga cgttttgagc cgtttcgttc
     3181 ctcgcattat ttcgttttat ttactcttgc tggcggcagg cggtacagct aacgcacaat
     3241 ctaccttcga gcaaaaagcg gcaaatccct ttgataataa caatgatggt ctgccggatt
     3301 taggcatggc acctgaaaat catgatgggg aaaaacactt tgctgaaatt gtgaaagatt
     3361 tcggcgaaat cagtatgaat gataacgggc tggatactgg cgagcaggca aaagctttcg
     3421 cattgggaaa agtccgcgac gcgcttagtc aacaggttaa tcagcacgta gagtcctggc
     3481 tatcaccgtg gggaaatgcc agtgttgatg tcaaagtgga taacgaaggt cattttaccg
     3541 gcagtcgtgg aagctggttt gtgccgttac aagataatga tcgttatctc acctggagcc
     3601 agcttggtct tactcagcag gatgatgggt tggtgagcaa tgtgggcgtt gggcaacgct
     3661 gggcgcgcgg caactggctg gtgggttata acacttttta tgacaacttg ctggacgaaa
     3721 atcttcagcg agcgggcttt ggtgccgaag cgtggggcga atatttgcga ctatcggcaa
     3781 acttttatca gccatttgct gcatggcatg aacagacagc cacgcaggaa caacggatgg
     3841 cgcgcgggta cgacctgaca gcccggatgc gcatgccgtt ctatcaacac ctcaatacca
     3901 gtgtcagcgt agaacagtat tttggtgatc gtgttgattt gtttaactct ggtacgggtt
     3961 atcacaatcc cgtcgcgttg agtctgggat taaattacac ccctgtgcca ttagtcactg
     4021 tgacggccca gcataaacag ggtgaaagtg gcgagaatca aaataacctc gggctgaatc
     4081 ttaattaccg ctttggtgta ccgctcaaaa aacaactttc tgcgggcgag gttgccgaaa
     4141 gtcagtcgtt acgtggtagt cgctatgata atccgcagcg aaataatcta ccgactcttg
     4201 agtaccgaca gcgaaaaacg ttaacggtgt ttctggcgac accgccgtgg gatctaaaac
     4261 ctggcgaaac agtgccgctg aaattacaaa tccgcagtcg ttacggtatt cggcaactga
     4321 tttggcaggg cgatacgcag atattaagtt tgacgccagg cgcacaagcc aacagcgcgg
     4381 agggctggac gctgatcatg cctgactggc agaacgggga aggggcgagc aatcactggc
     4441 gattgtcggt ggtggtggaa gataaccagg ggcagcgtgt ctcctccaat gagatcacgc
     4501 taacgcttgt cgaaccgttc gacgcattgt caaacgacga actgcgctgg gaaccgtaat
     4561 cagaaaatgc gctcctgatg cacccatacc gctgcttcca cgcgagactt gagcttcatt
     4621 ttcttcagca tgtgcttgac gtgcactttt actgtgcttt cggtgatatc caggcggcgg
     4681 gcaatcatct tgttcggcaa accctgggca atcagcttga gaatatcgcg ctcgcgtggg
     4741 gttaactggt taacatcgcg ctcagtagtg gcacggttag cgcgcaagct ggcggccaga
     4801 acaggcgtta atgcttcgct taataccatt tcgccagcag ctgcctgatg caatgctttc
     4861 agcagatctt ccggttccat atcttttaac agatagccat ccgcgccgcg tttcagtgcg
     4921 gtgaccacat cttcttcatg gttagagacg ctgaatacca caatgcgccc tgagagggac
     4981 ttttcgcgca gtttatccag cgtttccaga ccgttcatgc cgggcatatt gagatctaac
     5041 aggatcagat cgggatcaag agactccgcc agttcaatac cctgttcgcc attactcgct
     5101 tcgccaacca cggtgatatc tggtgccata ctgataagct gttttacgcc agttcgcagc
     5161 atcgggtgat cgtcaatcag caggatagta gccggttcct gattactcat gggtatctcc
     5221 ttggacgtct gtgaaagttt tttcgggaat aaaggtgacc accacttcgg tgccacctga
     5281 ttcacgacgg cggacgcggc aatcgcctcg taaactttgc gcacggtcgc gcattattat
     5341 catgccgtag tgattgctgc ggatggcatt ttcaggcacg ccgcagccgt tatcctggac
     5401 ggtcagtttg acctgattat cgttttgcgc caccgtcacc acgacctcac tcgcttgcga
     5461 atgtttgagg gcgttactta atgcctcacg ggcaatttgc aacaagtgga ttgcctgatg
     5521 tgaaggcacc agacgaggcg gcaattgata atccagcttc accgggaagc caaatttggc
     5581 gctgtactct tcgcaactcg cttccagcgc cggacgtaat ccaggctcgg tgagctgcaa
     5641 gcggaatgtg gtgagcaatt cacgcaactg cgcccaggat gcattcagtt cgttacggat
     5701 ctgacttaac agttcgcggc tgctttctgg cagcgcatcg ccctgcatct gtaaacaact
     5761 cacctgcatc ttcatgcaag agagagattg ggcaatagaa tcatgcagtt cgcgcgcaat
     5821 ggtggcacgc tcttccatca cgatcaactg ttgctgacgt tcctgatgcc gatccagcgc
     5881 cagcgtggcg gtgagttgtt caaccagggt atccaccagt tgttgttgat catggctaag
     5941 atgacgcccc tgcggtaggg tcgccagcaa aataccgtac tgcgtatgtg agtcagccag
     6001 ccgccacttc agggttgtgc cgcgatcgcc aacgggtaat acgccgcgcg ggcagagctg
     6061 gcagccttta tcatcacaag tcatatctgg ctggcaggta aactcctgat gattctcttc
     6121 atcatcagtg tcatacaccc gcaattcgat atcacgtagc agggttaaat tctgtaagcc
     6181 gttgagtaca ggtgacaggc gttcacacag cggggcgcgg gaatgcaaac ggcggttagc
     6241 ctgccataaa aaagagagga tctgattttt atgctccagc ccggcggttt tctcctgaac
     6301 ccgctgctca agtacggcat aactttcggc cagttctgca gacatattgt tcaatgcagt
     6361 tccaagcatc gccatttcgt tgcgcccgct gatgttcgcg cgttgggtaa aatcgcgatg
     6421 actgacggca ctcgccattg ccagcagttg ccgccacggt tgtagcagtc gcgcccgcaa
     6481 ccagataata gtgaacacca gtaaaagtgc cataaatacc gccattaccc gatggaccag
     6541 taccactgtc tcaatgcgca tttccgtggt gcggtcaaaa ccagatacca gctgatcaag
     6601 cccggcaaca aactggctga catccgctga caccgtttcg cggttttgtg cacgcatcag
     6661 tgcagggatc agttcattac gccagtaatc ttgtaaaccc tgtaattgcg ccagttgtcc
     6721 gtctcgttct gctgctcgag tcaactcggc gctaaatgcc gtttgttcca tctctttaat
     6781 taagggcttg tctttctcgc ttaatggcaa tgctgccaac agacggtaac tttgcatgcg
     6841 cagcgatccc gctttgttga tcgcatgggc gctgccctga acgccttgca ccagccagcc
     6901 agaaaccgcc atccctgcca gtccaatagc agtagaaagc aacacaataa gcgcaacctg
     6961 attaaccagg gtgagcggag agagacaacg tttaagcatg taaacctctt ccttcaggct
     7021 ttaaatgagc aataacctta atgaatgtga cgatacattc tggaatggca gtattctcgg
     7081 ctattggctg aagtataccc atacccggaa agagttactc cttatttgcc gtgtggttag
     7141 tcgctttaca tcggtaaggg taggaatttt acagcaccgt gaaaaatctc ataattttta
     7201 tgaaatcact gtactcacta tgggtaatga taaatatcaa tgatagataa agttatctta
     7261 tcgtttgatt tacatcaaat tgcctttagc tccagacact aaggtggcag acatcgaaac
     7321 gagtatcaga ggtgtctatg agtcactcat ccgcccccga aagggctact ggagctgtca
     7381 ttacagattg gcgaccggaa gatcctgcgt tctggcaaca acgcggtcaa cgtattgcca
     7441 gccgtaacct gtggatttcc gttccctgtc tgctgctggc gttttgcgta tggatgttgt
     7501 tcagcgctgt tgcggtgaac ttaccgaaag tcggttttaa ttttacgacc gatcagctat
     7561 ttatgttgac tgcgctgcct tcggtttctg gcgcgttatt acgtgttcca tactccttta
     7621 tggttcctat cttcggtggt cgtcgctgga cggcgttcag caccggtatt ctgattattc
     7681 cttgcgtctg gctgggtttt gccgtgcagg atacctccac gccttatagc gtcttcatca
     7741 tcatctctct gctatgcggc tttgctggcg cgaacttcgc atccagtatg gcaaacatca
     7801 gcttcttctt tccgaaacag aagcagggtg gcgcgctggg tctgaatggt ggtctgggaa
     7861 acatgggcgt cagcgtcatg cagttggttg ctccgctggt ggtatcactg tcgattttcg
     7921 cagtatttgg tagccagggc gtcaaacagc cggatgggac tgagctgtat ctggcgaatg
     7981 cgtcctggat atgggtgccg ttccttgcca tcttcaccat tgcggcgtgg tttggcatga
     8041 acgatcttgc tacctcgaaa gcctccatca aggagcagtt gccggtactc aaacggggtc
     8101 atctgtggat tatgagcctg ctgtatctgg caaccttcgg ctccttcatc ggcttctccg
     8161 cgggctttgc aatgctgtca aaaacgcagt tcccggatgt tcagattctg caatacgctt
     8221 tcttcgggcc gtttattggt gcgctggcgc gttctgcagg tggtgcatta tctgaccgtc
     8281 tgggcggaac tcgtgtcacg ctggtgaact ttattctgat ggcgattttc agcggcctgc
     8341 tgttcctgac cttaccgact gacgggcagg gcggaagctt catggcgttc ttcgcggtct
     8401 tcctggcgct gttcctgaca gctgggctgg gtagtggttc cactttccag atgatttcag
     8461 tgatcttccg taaactgaca atggatcgcg tgaaagcaga agggggttct gacgaacgtg
     8521 cgatgcgtga agcggcaacc gacacggcgg cggcgctggg tttcatctct gcgattggcg
     8581 cgattggtgg cttctttatc ccgaaagcgt ttggtagctc gctggcatta acgggttcgc
     8641 cagtcggcgc aatgaaggta tttttgattt tctatatcgc ctgcgtagtg attacctggg
     8701 cggtatatgg tcggcattct aaaaaataaa ccgttactcg tcatacttcg ggttacatgt
     8761 gctgcggctg cgttcattca ccccagtcac ttactttagt aagctcctgg gattcattca
     8821 cttgccgcct tcctgtaaac cgaattatat agagtaaaat atttgattat cctttgcgcg
     8881 gcatgatgtc gcgctttttt tatgcgttat ttagttacaa catactaatg ttatatgatt
     8941 tatttcgccg gatttcatta agagccatta atatgttacc catggggaat actccttaat
     9001 acccatctgc ataaaaatct taatagttta aataactaca ggtataaaac gtcttaattt
     9061 acagtatgtt atgtggtggc tgttaattat cctaaagggg tatcttagga atttacttta
     9121 tttttcatcc ccatcactct tgatcgttat caattcccac gctgtttcag agcgttacct
     9181 tgcccttaaa cattagcaat gtcgatttat cagagggccg acaggctccc acaggagaaa
     9241 accgatgagt aaattcctgg accggtttcg ctacttcaaa cagaagggtg aaacctttgc
     9301 cgatgggcat ggccagcttc tcaataccaa ccgtgactgg gaggatggat atcgccagcg
     9361 ttggcagcat gacaaaatcg tccgctctac ccacggggta aactgcaccg gctcctgcag
     9421 ctggaaaatc tacgtcaaaa acggtctggt cacctgggaa acccagcaga ctgactatcc
     9481 gcgtacccgt ccggatctgc caaaccatga acctcgcggc tgcccgcgcg gtgccagcta
     9541 ctcctggtat ctttacagtg ccaaccgcct gaaatacccg atgatgcgca aacgcctgat
     9601 gaaaatgtgg cgtgaagcga aggcgctgca tagcgatccg gttgaggcat gggcttctat
     9661 cattgaagac gccgataaag cgaaaagctt taagcaggcg cgtggacgcg gtggatttgt
     9721 tcgttcttcc tggcaggagg tgaacgaact gatcgccgca tctaacgttt acaccatcaa
     9781 aaactacggc ccggaccgtg ttgctggttt ctcgccaatt ccggcaatgt cgatggtttc
     9841 ttacgcatcg ggtgcacgct atctctcgct gattggcggt acttgcttaa gcttctacga
     9901 ctggtactgc gacctgcctc ctgcgtctcc gcaaacctgg ggcgagcaaa ctgacgtacc
     9961 ggaatctgc
//
LOCUS       DACAPB010000165         9953 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.165, whole genome shotgun sequence.
ACCESSION   DACAPB010000165 DACAPB010000000
VERSION     DACAPB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.165"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..314)
                     /locus_tag="IT904_003697"
     CDS             complement(<1..314)
                     /locus_tag="IT904_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP3193426.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FT"
     gene            complement(658..975)
                     /gene="bolA"
                     /locus_tag="IT904_003698"
     CDS             complement(658..975)
                     /gene="bolA"
                     /locus_tag="IT904_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAP3193427.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            1280..1858
                     /locus_tag="IT904_003699"
     CDS             1280..1858
                     /locus_tag="IT904_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP3193428.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            1902..3377
                     /gene="ampG"
                     /locus_tag="IT904_003700"
     CDS             1902..3377
                     /gene="ampG"
                     /locus_tag="IT904_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAP3193429.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            3837..4784
                     /gene="cyoA"
                     /locus_tag="IT904_003701"
     CDS             3837..4784
                     /gene="cyoA"
                     /locus_tag="IT904_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAP3193430.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            4806..6797
                     /gene="cyoB"
                     /locus_tag="IT904_003702"
     CDS             4806..6797
                     /gene="cyoB"
                     /locus_tag="IT904_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAP3193431.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            6787..7401
                     /locus_tag="IT904_003703"
     CDS             6787..7401
                     /locus_tag="IT904_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAP3193432.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            7401..7730
                     /locus_tag="IT904_003704"
     CDS             7401..7730
                     /locus_tag="IT904_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAP3193433.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            7742..8632
                     /gene="cyoE"
                     /locus_tag="IT904_003705"
     CDS             7742..8632
                     /gene="cyoE"
                     /locus_tag="IT904_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAP3193434.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            8781..>9953
                     /locus_tag="IT904_003706"
     CDS             8781..>9953
                     /locus_tag="IT904_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3193435.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWINGMFDGQGVFLAGAMLAAVWLTVTSTMKEP"
BASE COUNT         2139 a         2518 c         2573 g         2723 t
ORIGIN      
        1 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
       61 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
      121 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
      181 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
      241 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
      301 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
      361 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
      421 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
      481 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
      541 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
      601 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
      661 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
      721 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
      781 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
      841 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
      901 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
      961 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
     1021 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
     1081 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
     1141 cagtgtttct tttaccaggg gcgattattt cgtcaacatg atgaatttac atgccttacc
     1201 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
     1261 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
     1321 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
     1381 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
     1441 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
     1501 cgatctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
     1561 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
     1621 gtcccagggc aacgtgcgct acaacatcgc gaccaaagcg gatatcgcca tcatcgctac
     1681 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
     1741 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
     1801 cgctgatatg tctcaggaca ccagcatcca cgaattcatc aagcagaacg cgcgttaatt
     1861 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
     1921 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
     1981 cgctcgccct gacatccggt accttacagg cctggatgac ggtcgagaat attgatctca
     2041 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
     2101 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
     2161 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
     2221 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctcggcctct caggatattg
     2281 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
     2341 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcttagcc ctgtggctgg
     2401 cagataaatg gctgggctgg cagggcatgt actggttgat ggcggcgctg ttgatccctt
     2461 gtattatcgc gacgttgctt gcaccagaac cgaccgacac cattcctgtg ccaaaaacgc
     2521 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
     2581 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
     2641 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggtt aacaaaacgc
     2701 ttggcttact ggcgaccatt gtgggcgcat tgtacggtgg gattttgatg cagcgcctgt
     2761 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
     2821 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
     2881 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
     2941 agtcattttc cgcaactcaa tttgccctgc tctcagcgct ttccgctgta ggacgagttt
     3001 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
     3061 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
     3121 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
     3181 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta ctgctgttga
     3241 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
     3301 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
     3361 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
     3421 ttacggctaa ataaacatca gtatcgctta ataatatttg ttcttttgtg cggcttagcg
     3481 tttggaaatt attgacgcaa tttatgaatt ctattttttc ttacacgatt cagctaatga
     3541 gtctttattt tcccccaccc aattgtcacc ataatgataa ttatttgtta aacaattgtt
     3601 ttattccaga ttggttatac caattgcccg tccaaactcc ttctgcactc ccctttcgtt
     3661 ataacgccct tttgcaacag cttcttaaaa tcaacttgat atgttttgca acatatgtga
     3721 cctgacagcc aaatccaagt aacaggaatt taatcatgtt tacagtaatg taaccttccc
     3781 gtaaaatgcc cacacacttt aaacgccacc agatcccgtg gaattgaggt cgttaaatga
     3841 gactcaggaa atacaataaa agtttgggat ggttgtcatt atttgcaggc actgtattgc
     3901 tcagtggctg taattctgcg ctgttagatc ccaaaggaca gattggtctg gagcaacgtt
     3961 cactgatact gacggcattt ggcctgatgt tgattgtcgt tattcccgca atcttgatgg
     4021 ctgttggttt cgcctggaag tatcgtgcga gcaataaaga tgctaagtac agcccgaact
     4081 ggtcacactc caataaagtg gaagctgtgg tctggacggt acctatctta atcatcatct
     4141 tccttgcggt actgacctgg aaaaccactc acgctcttga gcctagcaag ccgctggcac
     4201 acgacgagaa gcccattacc atcgaagtgg tttccatgga ctggaaatgg ttcttcatct
     4261 acccggaaca gggcattgct accgtgaatg aaatcgcttt cccggcgaac actccggtgt
     4321 acttcaaagt gacctccaac tccgtgatga actccttctt cattccgcgt ctgggtagcc
     4381 agatttatgc catggccggt atgcagactc gcctgcatct gatcgccaac gaacccggta
     4441 cttatgacgg tatctccgcc agctacagcg ggccgggctt ctcaggcatg aagttcaaag
     4501 ctattgcaac accggatcgc gccgaattcg accaatgggt cgcaaaagcg aaacagtcgc
     4561 cgaacaccat gtctgacatg gctgcgttcg aaaaactggc cgcgcctagc gaatacaacc
     4621 aggtggaata tttctccaac gtgaaaccag acttgtttgc cgatgtgatt aacaagttta
     4681 tggctcacgg taagagcatg gacatgaccc agccagaagg tgagcacagt gcacacgaag
     4741 gtatggaagg catggacatg agccacgcgg aatccgccca ttaaaggggt tgaggaagaa
     4801 taaagatgtt cggaaaatta tcacttgatg cagtcccgtt ccatgaacct atcgtcatgg
     4861 ttacgatcgc tggcattatt ttgggaggtc tggcgctcgt tggcctgatc acttacttcg
     4921 gtaagtggac ctacctgtgg aaagagtggc tgacctccgt cgaccataaa cgcctcggta
     4981 tcatgtatat catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac gccattatga
     5041 tgcgtagcca gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca cctcaccact
     5101 acgatcagat cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg atgcctttcg
     5161 ttatcggtct gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac gttgcgttcc
     5221 cgttcctcaa caacttaagc ttctggttta ctgttgttgg tgtgattctg gttaacgttt
     5281 ctctcggcgt gggcgaattt gcgcagaccg gctggctggc ctatccaccg ctatcgggaa
     5341 tagagtacag tccgggagtc ggtgtcgatt actggatatg gagtctccag ctatccggta
     5401 taggtacgac gcttaccggt atcaacttct tcgttaccat tctgaagatg cgcgcaccgg
     5461 gcatgaccat gttcaagatg ccagtattta cctgggcatc actgtgcgcg aacgtactga
     5521 ttattgcttc cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg gatcgctatc
     5581 tgggcaccca tttctttacc aacgatatgg gtggcaacat gatgatgtac atcaacctga
     5641 tttgggcctg gggccacccg gaagtttaca tcctgatcct gcctgttttc ggtgtgttct
     5701 ccgaaattgc ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg ctggtatggg
     5761 caaccgtctg tatcaccgtg ctgtcgttca tcgtttggct gcaccacttc tttacgatgg
     5821 gtgcgggcgc gaacgtaaac gccttctttg gtatcaccac aatgattatc gccatcccga
     5881 ccggggtgaa gatcttcaac tggctgttca ccatgtatca gggccgcatc gtgttccatt
     5941 ctgcgatgct gtggaccatc ggttttatcg tcactttctc ggtgggcggg atgactggcg
     6001 tgctgctggc agtaccgggc gcggacttcg ttctgcataa cagcctgttc ctgattgcgc
     6061 acttccataa cgtgatcatc ggcggcgtgg tcttcggctg cttcgcaggg atgacctact
     6121 ggtggcctaa agcgttcggt ttcaaactga acgaaacctg gggtaaacgc gcgttctggt
     6181 tctggatcat cggcttcttc gttgccttta tgccgctgta tgcgctgggc ttcatgggca
     6241 tgacccgtcg tttgagccag cagattgacc cgcagttcca caccatgctg atgattgcag
     6301 ccagcggtgc agtactgatt gcgctgggta ttctctgcct cgttattcag atgtacgttt
     6361 ctattcgcga ccgcgaccag aaccgtgacc tgactggcga cccgtggggt ggccgtacgc
     6421 tggagtgggc aacctcttcc ccgcctccgt tctataactt tgccgttgtg ccgcacgttc
     6481 acgagcgtga tgcattctgg gaaatgaaag agaaaggcga agcgtataaa aagcctgacc
     6541 actatgaaga aattcatatg ccgaaaaaca gcggtgcagg tatcgtcatt gcagctttct
     6601 ccaccatctt cggtttcgcc atgatctggc atatctggtg gctggcgatt gttggcttcg
     6661 caggcatgat catcacctgg atcgtgaaaa gcttcgacga ggacgtggat tactacgtgc
     6721 cggtggcaga aatcgaaaaa ctggaaaacc agcatttcga tgagattact aaggcagggc
     6781 tgaaaaatgg caactgatac tttgacgcac gcgactgccc acgcgcacga acacgggcac
     6841 cacgatgcag gcggaaccaa aatcttcgga ttttggatct acctgatgag cgactgcatt
     6901 ctgttctcaa tcttgtttgc tacctatgcc gttctggtga acggcaccgc aggcggcccg
     6961 acaggtaagg acattttcga actgccgttc gttctggttg aaactttctt gctgttgttc
     7021 agctccatca cctacggcat ggcggctatc gccatgtaca aaaacaacaa aagccaggtt
     7081 atctcctggc tggcgttgac ctggttgttt ggtgccggat ttatcgggat ggaaatctac
     7141 gaattccatc acctgattgt taacggcatg ggtccggatc gcagcggctt cctgtcagcg
     7201 ttctttgcgc tggtcggcac gcacggtctg cacgtcactt ccggtcttat ctggatggcg
     7261 gtgctgatgg tacaaatcgc tcgtcgcggc ctgaccagca ctaaccgtac ccgcatcatg
     7321 tgcctgagcc tgttctggca cttcctggat gtggtttgga tctgtgtgtt cactgttgtt
     7381 tatctgatgg gggcgatgta atgagtcatt ctaccgatca cagcggcgcg tcccatggca
     7441 gcgtaaaaac ctacatgaca ggctttatcc tgtcgatcat tctgacggtg attccgttct
     7501 ggatggtgat gacaggggct gcctctccgg ccgtaattct gggaacaatc ctggcaatgg
     7561 cagtggtaca ggttctggtg catctggtgt gcttcctgca catgaatacc aaatcagatg
     7621 aaggctggaa catgacggcg tttgtcttca ccgtgctaat catcgctatc ctggttgtag
     7681 gctccatctg gattatgtgg aacctcaact acaacatgat gatgcactaa gagcggcggt
     7741 tatgatgttt aagcaatacc tgcaagtaac gaaaccaggc atcatctttg gcaacctgat
     7801 ctcggtgatt gggggattcc tgctggcctc aaagggcagc attgattatc ccctgtttat
     7861 ctacacgctg gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta acaactacat
     7921 cgacagggat atcgacagaa agatggaaag gacgaagaat cgggtgctgg ttaaaggcct
     7981 gatctctcct gctgtctcgc tggtgtacgc caccttgctg ggtattgctg gctttatgct
     8041 gctgtggttt ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct ttgtggttta
     8101 tgtcggcgtc tatagcctgt acatgaaacg ccactcagtc tacggcacat tgattggttc
     8161 gctctccggc gctgcgccgc cggtgattgg ctactgtgca gtaaccggcg agttcgatag
     8221 cggcgcagcg atcctgctgg ctatcttcag cctgtggcag atgcctcact cctatgccat
     8281 cgccattttc cgctttaagg attaccaggc ggcaaacatt ccggtattgc cagtggtaaa
     8341 aggcatttcg gtggcgaaga atcacatcac gctgtatatc atcgcctttg ccgttgccac
     8401 gctgatgctc tctcttggcg gttacgctgg atataaatat ctggtggtcg ccgcggcggt
     8461 tagcgtctgg tggttaggta tggctctgcg cggttataaa gttgctgatg acagaatctg
     8521 ggcgcgcaag ctgttcggct tctctatcat cgccatcact gccctctcgg tgatgatgtc
     8581 cgttgatttt atggtaccgg actcgcatac gctgctggct gctgtgtggt aacaaaacct
     8641 ctcagttaaa aaggtgctac ggcacctttt ttcttagcat tagaaacata tccctctcga
     8701 aatatttact aaaaaatccg catgtttacc ccattcgttt gccgctttac actagtcgcg
     8761 aatttaaaac agaggtggta atgaacgatt ataaaatgac gccaggtgag cggcgcgcga
     8821 cctggggttt aggaaccgtt ttctcgttgc gcatgctggg catgtttatg gttctgccgg
     8881 ttctgaccac gtatggcatg gctctgcaag gtgccagcga agcattaatc ggtattgcca
     8941 ttggtattta tggtctgact caggccgttt ttcagattcc gtttggcctg ctttccgacc
     9001 gcattggtcg caaaccatta attgtcggtg ggctggcggt gtttgccgcc ggtagcgtta
     9061 tcgctgcgct ttctgactcc atctggggaa ttattctggg ccgggcgcta caaggctccg
     9121 gtgcgattgc cgctgccgtt atggcgctgc tttccgatct cacgcgcgaa caaaaccgta
     9181 ccaaagcaat ggcgtttatc ggcgtgagct ttggcattac ctttgccatt gcgatggtgc
     9241 ttggcccgat catcactcac aaacttgggc tgcacgcgct gttctggatg atcgctattc
     9301 tggcaacgac cggcattgcg ttgaccattt gggttgtgcc caacagtagc actcacgtac
     9361 ttaatcgtga gtccggaatg gtgaaaggca gtttcagtaa agtgctggcg gaaccgcggc
     9421 tgctgaaact caactttggc attatgtgtc tgcatatttt gctgatgtcg acgtttgttg
     9481 ccctgcccgg acaactggct gatgcagggt tcccggcggc tgaacactgg aaggtctatc
     9541 tggcgacaat gctaatcgcc tttggctcgg tcgtgccttt cattatctac gctgaagtta
     9601 agcgcaaaat gaagcaagtc tttgtcttct gcgtcgggtt gatcgtggtt gcggaaattg
     9661 tgttgtggaa cgcgcaaacg cagttctggc aactggtggt cggcgtgcag cttttctttg
     9721 tggcgtttaa tttgatggaa gccctcctgc cctcacttat cagtaaagag tcgccagcag
     9781 gttacaaagg tacggcgatg ggtgtttact ccaccagcca gtttcttggc gtggcgattg
     9841 gcggttcgct gggcggctgg attaacggca tgtttgacgg tcagggggta tttctcgctg
     9901 gcgcaatgct ggccgcagtg tggctgacag tcaccagtac catgaaagaa ccg
//
LOCUS       DACAPB010000166         9892 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.166, whole genome shotgun sequence.
ACCESSION   DACAPB010000166 DACAPB010000000
VERSION     DACAPB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.166"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            633..1136
                     /locus_tag="IT904_003707"
     CDS             633..1136
                     /locus_tag="IT904_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAP3193436.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(1215..1466)
                     /locus_tag="IT904_003708"
     CDS             complement(1215..1466)
                     /locus_tag="IT904_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAP3193437.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMWAIQKAIALIELKFTPQGESH"
     gene            complement(1581..1667)
                     /gene="azuC"
                     /locus_tag="IT904_003709"
     CDS             complement(1581..1667)
                     /gene="azuC"
                     /locus_tag="IT904_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAP3193438.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1930..2253
                     /locus_tag="IT904_003710"
     CDS             1930..2253
                     /locus_tag="IT904_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193439.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2424..2921
                     /gene="ftnA"
                     /locus_tag="IT904_003711"
     CDS             2424..2921
                     /gene="ftnA"
                     /locus_tag="IT904_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAP3193440.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2959..3198)
                     /locus_tag="IT904_003712"
     CDS             complement(2959..3198)
                     /locus_tag="IT904_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAP3193441.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            3389..4600
                     /gene="tyrP"
                     /locus_tag="IT904_003713"
     CDS             3389..4600
                     /gene="tyrP"
                     /locus_tag="IT904_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAP3193442.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4662..5327)
                     /gene="yecA"
                     /locus_tag="IT904_003714"
     CDS             complement(4662..5327)
                     /gene="yecA"
                     /locus_tag="IT904_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAP3193443.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5523..5609)
                     /locus_tag="IT904_003715"
     tRNA            complement(5523..5609)
                     /locus_tag="IT904_003715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5573..5575),aa:Leu,seq:taa)
     gene            complement(5622..5695)
                     /locus_tag="IT904_003716"
     tRNA            complement(5622..5695)
                     /locus_tag="IT904_003716"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5661..5663),aa:Cys,seq:gca)
     gene            complement(5750..5825)
                     /locus_tag="IT904_003717"
     tRNA            complement(5750..5825)
                     /locus_tag="IT904_003717"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5790..5792),aa:Gly,seq:gcc)
     gene            complement(5977..6525)
                     /gene="pgsA"
                     /locus_tag="IT904_003718"
     CDS             complement(5977..6525)
                     /gene="pgsA"
                     /locus_tag="IT904_003718"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP3193444.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6582..8414)
                     /gene="uvrC"
                     /locus_tag="IT904_003719"
     CDS             complement(6582..8414)
                     /gene="uvrC"
                     /locus_tag="IT904_003719"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP3193445.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8411..9067)
                     /gene="uvrY"
                     /locus_tag="IT904_003720"
     CDS             complement(8411..9067)
                     /gene="uvrY"
                     /locus_tag="IT904_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAP3193446.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            9363..9539
                     /locus_tag="IT904_003721"
     CDS             9363..9539
                     /locus_tag="IT904_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193447.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9526..9750
                     /locus_tag="IT904_003722"
     CDS             9526..9750
                     /locus_tag="IT904_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAP3193448.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAIGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9818..>9892)
                     /locus_tag="IT904_003723"
     CDS             complement(9818..>9892)
                     /locus_tag="IT904_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="regulates genes involved in cell division; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAP3193449.1"
                     /translation="NMQKKINAPNKTQVACYAAATGLI"
BASE COUNT         2534 a         2447 c         2262 g         2649 t
ORIGIN      
        1 agaattacag tgagaacgtg cataaattta gcgggaaaag acataaggga aagccaattt
       61 gtcagacaaa ttgtcgaatg cacagcagat taatccataa gattagcctg gaaatccttg
      121 ttgtctttgg tacccatgcg ggatgtcttc tttttaacca gtcaataggc cgcattacct
      181 ggcgttgagt ttttgaaatg gtgtaataac cgcaactcaa agatgtggaa aatgcacgtc
      241 attcatttcg tcattaatta tcactgtgct cattaattaa cagaacacgt ataatgagag
      301 ccatctcgca aaaatgaaaa aacgttttat aaaatcatca cttcatcatg aattcaaatt
      361 cattgattaa tatcaacaag atacaaaaag cactatcatt aaaattcatt gcagttacat
      421 tgatttcatc aatgaaatgt aaaaatatat aaacttgatg atttaagcat tttcttatac
      481 ccgttcagac gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc
      541 ggaccggcag aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca
      601 cctctccact tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac
      661 tcaactctca aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact
      721 ggtgttctga acagagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta
      781 atgtgaccca aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg
      841 ttaaagccat tgatgttccc ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa
      901 cgatggaaga atacgagcaa cgttctagta cgttggcaca gttagccgat gaagcgaaag
      961 aactgaatga tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg
     1021 atggtctgtt gctgcaaacc attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt
     1081 gccctgtgca gaccgaccaa catgttctga atgtcgtgtc acaccagctg cattgatcat
     1141 catcggcgct aatgcattgc gccgatgaag gttttgagaa accgctgcct catctgtttg
     1201 aagcagcggt ttttttaatg ggattcaccc tgtggggtaa acttgagttc aataagcgcg
     1261 atggcttttt ggattgccca catggtgacc gggtctgcgg cggcgggatg gttagtaaag
     1321 tcgatattct tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg
     1381 agaacatcca gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc
     1441 tgatcggcat tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat
     1501 agtagcggct tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac
     1561 ctgccggagc aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc
     1621 ttgcaatagt tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga
     1681 ctatttgtta tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat
     1741 caaactttat gtgatacaaa tcacataaat acccctttaa tgttataaaa atgataatca
     1801 aaaaacagcc cccctatttc tgacacctac agatggcaag aaatagcgcc tgccaggcgt
     1861 cttttccggc cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc ttactcttct
     1921 ggctggacca tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg
     1981 gttactcgcc aggcccatgt gagcgaagtt gacgcggcaa caggtattgt acggttggtt
     2041 tatgaccagg cttttttgca gcatgcccat accgatcgtt atgtcagtcg cgggatcgcc
     2101 gatcgcgcat gccagcagga aggctatacc cacgcggtcc cctttggtca gccagtaggc
     2161 aactgtagcc tttttgcggg ttccttatgt ttgaatacgg aattcacttt gtcatatcag
     2221 tgccatcatt ccgccttccc tgtctttctc taaatatttt aatcagcgag gggatcttcg
     2281 ctgattaaag aaatagcggt aatgcgtttt attcccattc gtatttttaa taattgaagt
     2341 ttatatttta ccttttgcaa ataataaaat aacaaattat agtgacgcca cagcaacaaa
     2401 tataaccttt gtggagcact atcatgctga aaccagaaat gattgaaaaa cttaatgagc
     2461 agatgaacct ggaactgtac tcttcactgc tttatcagca aatgagcgcc tggtgcagct
     2521 atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc
     2581 atatgcagcg tctgtttgat tacctgactg ataccggcaa tttaccgcgt attaataccg
     2641 ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt attccaggaa acctataaac
     2701 acgaacaatt aatcacccag aaaattaacg aactggctca tgctgcaatg accaatcagg
     2761 actacccaac atttaatttc ctgcagtggt atgtttctga gcagcatgaa gaagagaaac
     2821 tgttcaaatc gattattgat aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt
     2881 ttatcgacaa agaactctct accctcgaca cacaaaacta atgctctccg gcggcaggtt
     2941 tactgccgcc gtttcaaatc agtgacggca aatcttactc tgatcggtcg aaaaaccgtc
     3001 tttcgaaggt ataaatttgc cttttgctgc cagaaacgct accagctctc ccgctgtcat
     3061 cccttctgcc gagcaggtgt gaaaacgtgc ctgttcacca aaacgcgctt taatcgcagc
     3121 ttccagactg gcatgcgtat attgctcgcc tgattcaatc atcatattta acacttcatg
     3181 accgtgaata gagtccatcg tcccttctca aaaaaagcct agcgtagcga ttgccgctta
     3241 tgaagacttt gcgccagcgc aggactgaat gctttttatt gtacatttat atttacacca
     3301 tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc agatatcact
     3361 cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa gtgtttttat
     3421 cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg cggccggtgt
     3481 tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt gctacacggc
     3541 gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg gcacgctggc
     3601 aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga tgttcttaat
     3661 gtatgctctg actgcggcat acatcagcgg tgccggtgaa ttgttggcct ccagcatcag
     3721 cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc
     3781 cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt ttctgttcag
     3841 cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt
     3901 gaatctttta accctgccgt tgcaacaggg gctggctctg tcagcaatcc cggtgatttt
     3961 tacctcattt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacgt
     4021 tcgtaagcta cgctgggtgt ttataaccgg tagtgcgatc cccctggtgg catatatttt
     4081 ctggcaggtg gcaacgcttg gcagcattga ttcaacaacc tttatgggat tgctggctaa
     4141 tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt
     4201 tgagctggca gtgcatttat ttgctgattt agccctcgcc acgtcatttc tcggcgttgc
     4261 gttaggctta tttgattatc tggctgattt atttcagcgt tcaaataccg ttggtggacg
     4321 gttgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt tttatccacg
     4381 aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat
     4441 cccttcgctg ttgacctggc aaagcagaaa gcacaatcct caagcgggtt accgggtcaa
     4501 aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca
     4561 atttttgatt gcggcagggt tgttaccaga agtggggtga ttagatagcc tcaaattcct
     4621 tattgggtgc cagaattaac gctgacaccc aatttggcct cttaatgcag gcagcactgc
     4681 ttaaatttct taccactacc gcacgggcaa ggatcgttac gccccggttt ctcttctgct
     4741 ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtacgc atgtagatca
     4801 agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga cattttctct
     4861 acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc tggttttaac
     4921 gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac
     4981 tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac
     5041 tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc
     5101 ataaagcgcg tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac
     5161 acggcaacca gccactgttc cggttcaatc tcttgcggag aactcaacac cgccgtcaat
     5221 aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt ggtcagaata
     5281 tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg
     5341 tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga
     5401 gtggctttgc tggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa
     5461 aaccacccga aggtggtttc acgacactgc ttattgcttt gattttattc ttatctttcc
     5521 catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc
     5581 cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt
     5641 cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt
     5701 cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa
     5761 acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt
     5821 cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctag caatcagtgc
     5881 cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc
     5941 acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca aatctgcacg
     6001 cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag
     6061 tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac
     6121 catctgggca gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc
     6181 caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc
     6241 cgccggtaag gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc
     6301 cacgagaact ttatctgcga cagggtcaag gaaagcacca aaccgggtac tctggttcca
     6361 gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc
     6421 ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag
     6481 gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta
     6541 tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag
     6601 aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg
     6661 gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg gcgttttggc
     6721 ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg
     6781 tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg
     6841 ggagaatctg gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc
     6901 ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat
     6961 gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg
     7021 ataagtatca catccgggat cttactgtcg tcgatggctt taccataacg ccgacgcagc
     7081 acctgattca tcgccgcata atcatcgccc ggcgtgatgc cagtaatgtt atagcgccga
     7141 tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttca
     7201 cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacaca
     7261 ctggcaagtg ctgtaagccg ctggtgaacg gtagattgct gcgaaagttt gctggttaag
     7321 gccgtcgccg cattggtgcg cgcgagtttc agataacgcg cccgatcgcc gcgaggtttg
     7381 gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta
     7441 tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt
     7501 aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact
     7561 ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca
     7621 caagccatgc ccgcatcgaa cgccacacca ataacgtcga ggtcgtcgcc ggtattggaa
     7681 acgaattgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgccgct
     7741 tcttcaaact ccagattctg gctggcagtt tccatacgac taatgagttg cgtaagcacc
     7801 tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct
     7861 tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac
     7921 ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc
     7981 agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct
     8041 ttggcatgct tcgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag
     8101 ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg
     8161 atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gagtaaccgt tacatcaatt
     8221 tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag
     8281 ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca
     8341 tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca
     8401 aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca
     8461 ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc
     8521 tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct
     8581 tggtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg
     8641 ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa gcaatgtaac
     8701 gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc
     8761 tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga
     8821 cggtaagcat gatgattttg acatcagctg tggaacgcgc gattttacgc gtcgcctcaa
     8881 gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgcccgac
     8941 accacttaac ggcgtcttca ccgcacgatg cctcaccgac gacttttata ccctttatat
     9001 cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt
     9061 tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt cattcgtata
     9121 ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc
     9181 gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga
     9241 tcgtttcaca gtaagccctg cgacgaaatg tgacaaaaat tacctttatt cagcaaaaat
     9301 gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat
     9361 tcatgataaa aatatttatt ggtcattata ttaacgcatt taatagcacc gttgacgata
     9421 cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa
     9481 catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat
     9541 ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg
     9601 ctgacgctga tgctgcaggc gataggacaa gctgacgcgg gccgcgtgat gttaaagatg
     9661 gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc caaaacggtt
     9721 aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgatagcg tgcctttcag
     9781 ccggtttttg catctggcac gcaggacaga aaagagatca aattaagcca gtagcggccg
     9841 cgtaacaggc aacctgggtc ttattcggtg cattgatttt tttctgcatg tt
//
LOCUS       DACAPB010000167         9839 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.167, whole genome shotgun sequence.
ACCESSION   DACAPB010000167 DACAPB010000000
VERSION     DACAPB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.167"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(1..5528)
                     /gene="yeeJ"
                     /locus_tag="IT904_003724"
                     /pseudo
     CDS             complement(1..5528)
                     /gene="yeeJ"
                     /locus_tag="IT904_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(5842..5917)
                     /locus_tag="IT904_003725"
     tRNA            complement(5842..5917)
                     /locus_tag="IT904_003725"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5882..5884),aa:Asn,seq:gtt)
     gene            complement(6018..6815)
                     /gene="mtfA"
                     /locus_tag="IT904_003726"
     CDS             complement(6018..6815)
                     /gene="mtfA"
                     /locus_tag="IT904_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAP3193450.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            6909..6998
                     /locus_tag="IT904_003727"
     tRNA            6909..6998
                     /locus_tag="IT904_003727"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6943..6945,aa:Ser,seq:cga)
     gene            complement(7111..8085)
                     /locus_tag="IT904_003728"
     CDS             complement(7111..8085)
                     /locus_tag="IT904_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3193451.1"
                     /translation="MLNEWMPKGVFFRPSGYYWKPGGTTENLAPANASKAEVWLAYEK
                     VVEGRKKLLTFQQLWKKFLNSADYADLAPRTQKDYLAHEKYLLAVFGDAEAKAIKPEH
                     VRRYMDARGKRSRVQANHEHSSMSRVYRWGYQRGYVPGNPCVGVDKFPKPQRDRYITD
                     EEYIAIFTHATPAVKAAMEIAYLCAARVSDVLKMNWNQILDKGIFIQQGKTGIKQIKA
                     WTERLSAAVDICREWGQDGPVIRTMYGERYSYKGFNEAWRKARNAASEELGRPLDCTF
                     HDLKAKGISDYEGSGRDKQKFSGHKTESQVLVYDRKVKISPTLNKKMR"
     gene            complement(8075..8233)
                     /locus_tag="IT904_003729"
     CDS             complement(8075..8233)
                     /locus_tag="IT904_003729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193452.1"
                     /translation="MSNVTKPTSKGKFDGAVDYLCSDEARFLVMRGDYSEADIIQASV
                     SQDVIDAE"
     gene            complement(8361..9146)
                     /locus_tag="IT904_003730"
     CDS             complement(8361..9146)
                     /locus_tag="IT904_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193453.1"
                     /translation="MAKDSKLVYGASGKTNVLAFEPEKLHLVTDKTHPLYDERIHLPI
                     SEAMVLNIMDQGVLEPIIVWKDPETGLSCVVDGRQRVRHTLEANKRLSKLGKEPLLVP
                     GVPKRGSAIRMAQAMVSANEIRQADTPLGRAKKMADALERGHDEDDLALMFGVSVQTV
                     RATLSLLDATQAVRDAVESGTVTVTQARQLASLKPEEQREKVKQIETATAGTTGHEKA
                     RRQRQILGDAKPRLKTRKEITKALEDASGEYAEALRWVLGEAQ"
     gene            complement(9146..9445)
                     /locus_tag="IT904_003731"
     CDS             complement(9146..9445)
                     /locus_tag="IT904_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193454.1"
                     /translation="MAKTAKQLIKQAYEIAKTMPPEQAAIIKELATVLDVSNVALRQT
                     RTERDALLAEVKSWAKECDRLTERHTKNRTNMHVLEAMRDLKAICPTSFRNVEAL"
BASE COUNT         2279 a         2472 c         2383 g         2705 t
ORIGIN      
        1 agggatgctt tcaccatata cgaaccgttt ctttcgggcg tcatggtgac ctcggctaca
       61 ccctgcgtat tagtagagac cgtattctgg ctgatgatca tttgcgagga tggctctgca
      121 ctgaatgtca cgggttgatg cgcaaccggg ttgccaaact ggtcgttaac gtgagcttta
      181 actgctacgt cttgtgcgac cattacctga ccttccggta tctccagact ggtaatcgtc
      241 gcagaattaa catctgcact ggcattcagt aatcgcgaga tgacttcagt aggtttatct
      301 gccagagagg ctgaaactgt tttcagtccg acctcggtgc ttgtcacaag aatttcagcg
      361 aaaccccgat catccgtttc aacgctggtg ctgcttagcg tgacggaggt tccgcggaaa
      421 ttaactttta tgccttctac cgggttgccg ttagcgtcag tgacgcttgc cgtcatggtt
      481 gcgccctcgg tcgtgctgac gacgaatgag tataaagagg ttaaggaggc tagtgttgcg
      541 gtaccagcat cggcaatcaa agtcacctgt ttcgtatcac tgacaccata attgttcacg
      601 ctaactgtca cggggtaggt gccggatttt gtaccgctca gtgtaacttc agccttgccg
      661 ttgatatcag taatggcgga accgccttgt ccgagagtga agcttgcgct cacgtctgcc
      721 ggtagcgtga atgtcaccgc ctcattggct aacgggttgc cgtttccatc agtcactgtt
      781 gcctgaagtg tggtcatccc aacgttattg gcgataaagt tgtcctcggt aacgttaaga
      841 ttaacctgcg cactgagcgt atccgcaata aagttcacca ccaactgctc actcttaccg
      901 ccagtcatcg atgctgtaac agtatgagcg cctgcttttg tacctttcag cgtgactttc
      961 gctttgcctt cagcatcagt aatcgcttta ccgccatcgc tcagcgtgaa gttcgccttc
     1021 acatcttccg gcagagtaaa tgttacttca gtgttggcta tcgcattgcc ctctgtatca
     1081 gcgactgttg ctgttagtgt cgtgagatcg ttattgtcgg caatcaccgg atccttcgag
     1141 gctgccagcg tgatttcggc attcgcgacg ttcggcacat aggtcactgt ttgttgcatc
     1201 gaatcgccat tttcgagggt tgccgtcact tgataaaccc cggcttttgt agcggtaaag
     1261 cttgcgtaaa aattgccgtc gtggttggta gtatatatag cgttattact gggggtcacg
     1321 ccttcacttg gtgatactct gagggttacc tcctgctggg gtacaccgtt gccgtaatta
     1381 tccttcacct caatatacag attggtagtg tcgcctgcgg agactgtatc aaaaagtgcc
     1441 tgtagcaagg tgagatgtgc cgtagacgca tcagcgttga cattaattga tgtgctaagc
     1501 gtggagctac cattttccag actggcctca acggtatccg gtctcgctcc tgattctatc
     1561 gaggagcggg tattggtata agtgacggta gccttaccct gttcgttcgt tacggcttgg
     1621 ccgccattcg tcatttcggc agagtttgtt ctgctggtga agttcacagt cacacctttc
     1681 accggaaagc cattattatc aacgactgtg gcggtgatga cgctgccgga gctgttctgc
     1741 ggggtaccgg cgattatgct gtctgggaca ggcgtcaact cgataatttt tgccgctgct
     1801 gtgtcgccaa taaaatgcac agttttgttg tcgctggcac cattattagc cagtgatgca
     1861 gtgaccgtct gctcaccaaa ggcaacgcct gcgagagtgg cctgcgcgat gccagactca
     1921 ttggtattac ttactcccgg ggtcagcgtg agtcctgaag aggctgagct gaatgttacc
     1981 ggaagattat tcacctcatt gtcgaactga tctttaaccg ttgcagttag cgttgcgtta
     2041 tcgacgccat tacctgtgat ctcatctttt gacatctgca ggacaacctg agccgaggtt
     2101 ttgtccgcca caaacgttac cggctgcgaa tcactggtat tgttattacc cagcgttgcg
     2161 gtaaccgtat gcgtacccgc ttttttacct ttcagcgtga catgcgcttc cccattggct
     2221 tgggtgatgg caatgccgtt attttcgagg gtaaagtttg ccgcaacgtc ctgtggcatg
     2281 gtgaaggtca ccgttattcc cgccaccgga tgattcgacg gatctttcac tgttgctgtc
     2341 agagttgtct catccacgcc attcccaatg atttccgctt tcgatgtttg cagaacgaca
     2401 accgctctgt ctttatccgc cacaaacgtc attggctgtg tatcgctgac attgctgtta
     2461 gccagacgag ccgtgatcat atgcgtgccc gctaacgtgc cggtcaggga ggcgattgca
     2521 accccgttac tatccgtcat gccttttcct ccgttgctaa ttgagaactg acttgcgacg
     2581 tcgtttggca cagagaaggt gatttcttta tctattagtg ggttgccatt tttatcctgc
     2641 aaggttgcag tcatatgtag cggagctgtg ttggtgacgg tgatatcacc tgaaggcaca
     2701 ctgagggtca gggctgcagt actttgatca ccgataaaat tcacctgttg attagcctgg
     2761 gaaccagagc tcacagaggc cgtaacccta taatcaccat ttttcaaact ggtcagctta
     2821 gctgtggcga tcccgtcgtg gctattcact tcggtttggc tcagtttcgc ctctgctgaa
     2881 ttaacattga aagtgacctt gacgtcattg agcaggttgc cttttgcatc ccggactgtc
     2941 gcggtcattg tggcgctatc attgccgtca gcgaccactt catttttcga cttctgcaga
     3001 tcaacctgcg cggtactcga gtcgccgaca aaactgacaa ttaacgtttg tttcacgcca
     3061 ttttcaaggg tgacttcaac cgtgttgtct tcctgcttac tactttacag atcaaaagtc
     3121 gccagaccat taacatccgt ttttgcggtg ttttgattgt taaagcaagt tgccgacccg
     3181 cttaataccg caaacgtgac ggtatgatca ttgattgggt tgcttccttc atcagcgaca
     3241 ttgaccgaga cggtgtttgc tgcattctca ttggcgagca caccattatt gctggcagat
     3301 aatgtcgcaa tttttgctga ctgcgggttg gcgtcgatga taaatccagc ggtatgcaaa
     3361 tcttcattcc agttttgcat taacagcttc gtagtaagcc cactgcctct ggtataggcg
     3421 gtataggttg ccttatagac gccatcagcg gtttctttcc agtctgtagt gacaccaggt
     3481 ttcacgttgt cgatgctgac tgcggtattc agttgctgtt tttgctcctt aacaggtttg
     3541 tcattttcat ctctcagttc taccgtcacc tcgataggat tgccggagag ataactgtcc
     3601 ttatcaatct tatttgacga gtgagttcgg gatgacgaaa cagaaatgat attaaccacg
     3661 gcgggggctt tagccgcatc cacaccattc agctgtggca tcagcgtcag cgtgccagac
     3721 atcgctcctg tggtcaggac ttgggtatag cttccgtcac cattatcttt ccagtcagaa
     3781 agggtgatgt cctgaacacc ttcgtgacgc gtcgagagca ccagcccgac aacaggatta
     3841 cctgctgcat catgcgcaat aaaagtcagt gtggcggttg aatgggaatc cgcgttcaat
     3901 gtttgggtac ttaacgatac cgaggaatct ttctggctta gcgtaggtgc ctgaacgacc
     3961 accatgctct gttcacgatt cgaaaaattg cctttgacat cttcagcggt gacttcaatc
     4021 ggccaggtgt tatcggtttc tggcgtactg gtgaaccggt acgccggcag ggtaaccaga
     4081 atatctttac ccgttgtaac cactttacca ccggcagctt ccagagcggt ggcttcgacg
     4141 ttatagcctt tcagggcata tttggtttgt agcgacgaaa ccagtgattt cacttctcct
     4201 gacttccctg tcacggggtc tgtcagggtc aggcggacca gttctttttt gcgatattcc
     4261 agaacgatgt tgttgttgcg atccaccagt tcataacggc tgcctgcaag gctacgccgt
     4321 gcagcgactt cattcgggtc aagctgtttc tgcattgcgc tgccaggttg ccaggtaaaa
     4381 tcgacggcaa atcgggtgtc attttcgccc tgtttaccct ggcgttgctc cgcgctaaag
     4441 gtcatcagcg ggaagggggt atagtttagt ccagcggtta tggcatgagg attactttgc
     4501 cgatcgtctt tatcgaacag ggccacttca tcgccataat actgttcata gaccagttta
     4561 ccgccaaggt gcggccaggc gggtaaccag ccttctgcgc gtacatccca gccattggcc
     4621 gggcgtgctt cataatcgtt gtccagttca ggtgcgctgc gccagttggt cagtcgcaaa
     4681 tagccgttac tgcttaattt aagatagtcg cgccagtact ccgcgccaat gccggcgcgg
     4741 gagtggtaac ggctaagatc gtggtcgaaa aagaagttga tgcccgacat ccatgtggga
     4801 gtgaaatgac gccaacctaa gccgttgtta atctgcgtac gctcgtcagt acgatggaga
     4861 gtatgctgac tgaaaaagag attatcaggc gtttcatacc acggatggag aaaatcgaac
     4921 tgggagttct tcaggctaaa atcttcatcc acgcccagcg tgattcttgc ggtaccgaag
     4981 cggcttaacc agtctgtcat tgcgcctgaa gcctgagaag aggcccatcc acgcgccata
     5041 tttgccgctt gctcgctgtt catatcttcg gcgagcagag acccgatttg ctgtgaagta
     5101 ctggctatct gttgctcaag gttgccactg ctattacccg gtggcggggt taaattattt
     5161 tcactaactt gtgccgggac atccagttca tcaccctggc ggacattatc aaaacctcga
     5221 gcaaacgtac gaaactggtt gagtttgcgt aactcagcca ccgaaatacc gaagcgttcg
     5281 gcaacgcttt gggccgattc cagcgctcca agggtatagg gtaccgtatt ggcatttgca
     5341 atggcgaatt gtgcaggaac tggttgttgg gttgcggcgt ttaccacacc ttgtgctgcc
     5401 gcagccatag ggaacgcaag ttgagttatc aggcagatac ccgcagttaa gcggcgtagt
     5461 ttaattccca tcccgcataa tatttgtcgg tcatttattt cttctccact tctcttcttc
     5521 gtagccatgc ttaccttccc tgaacgagct aacataaatg cgccctttct agcacagcga
     5581 aaaaatgtaa atcctgatag caatatgatt gaaaattaat aacttttcgt taggcagttt
     5641 tgagtgtgag ttgcaagagg ggagactact gaataactca agttttataa tcgaggggaa
     5701 aatggtgatg gcgttcatag caaaacgccc tcaaccataa aggtcgaggg cgcttaagat
     5761 gttaaaaacc cgctatccgt taaaaaacaa tgttcaacta aggtcagtga cattgcgcta
     5821 aaaaagcgaa tttttagaat ttggctcctc tgactggact cgaaccagtg acatacggat
     5881 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgtgaacgg ggcgcatatt
     5941 acttagcggt accttgtctg tcaacactaa atttcataag tcatttcaat tggttaatta
     6001 atctgcaaag ttgttaatta atgaacattc gtcgccgaaa acgagtctgt atcattagtg
     6061 cgatgcagtc tctgcaaagg atcttgttga taaaattggc agaaacgttg ccacaatgaa
     6121 gggaaacgag gagcaaaaag ttctggggcg ctaaagaaat attcagaaag tacggcaaaa
     6181 cattcagcag gatcactggc agcataagca tcaatgctcg ccgcattctc gccaaccaat
     6241 tcgatttctt cctgaatgtt gttcattgca gcatgaagat cgtgttccca gccagcaacc
     6301 tcacgcaacg gaataaaggg aactccgctg gcgcgatcgc cgttacgggt gtccagctta
     6361 tgagcgactt catgaataat caggttaaaa ccagaagcat caaaagaatc ttgtatatcc
     6421 aaccagttca aaacgatagg cccttgctgc cagctctgac ctgactgaac aatacgttgg
     6481 ttatgcacca gaccgatatc gtcttcccat tcatcatcga ccacaaatgg cgcaggataa
     6541 attaagactt catgaaaacc atccagccat tccagtccta actccagaac gggtaggcaa
     6601 aatagaagtg ctatccggca gcttcttaat gaatccagtt caaagccctg taaaggaaca
     6661 agccgctttt gctgtaaaaa acgttcggca agagtgacta atttgctttg ttcctgttcc
     6721 gtcagacccg ttaaaagggg gatcgatagt gcttcctgcc agggaagggc agtttgatgt
     6781 gctgattctt gtactttcca gggccactta atcatcgttt tgctcgcaaa ctcgtcactt
     6841 gaacaaaatt gcacggacag ggactgttaa aatgccaaat ttcctggcat catggcaacc
     6901 atctgaacgg agagatgccg gagcggctga acggaccggt ctcgaaaacc ggagtagggg
     6961 caactctacc gggggttcaa atccccctct ctccgccact attcaaacac ttacagcatt
     7021 ccctttcagt gacctgcatc ccgatgagaa aaattgagaa aatccactga gaaaaattct
     7081 agcgccgaag catgctccga cgccaaagaa tcatctcatt ttcttgttca acgtggggct
     7141 gattttaact ttcctgtcgt aaacaagtac ctgtgattct gtcttatgac cactgaattt
     7201 ttgcttatcc ctgccagatc cttcatagtc tgagatccct ttagccttta gatcatggaa
     7261 ggtgcaatca agtggcctac caagttcttc agaagccgcg tttctcgctt ttctccatgc
     7321 ttcattaaat cccttgtatg aataacgctc accatacatt gtcctgataa cagggccatc
     7381 ctgtccccat tccctgcaaa tatccacagc cgcactaaga cgctcagtcc aggctttgat
     7441 ctgtttaata ccagttttac cttgctgtat gaaaattcct ttatcaagaa tctgattcca
     7501 gttcatttta agaacatcag atacccttgc agcgcaaaga tacgcaattt ccatcgcggc
     7561 tttaactgca ggtgtcgcat gagtgaagat agctatgtac tcttcatcag ttatgtagcg
     7621 gtcgcgctgg ggtttaggaa acttatcgac accaacacac ggattacctg gtacataacc
     7681 acgctgataa ccccagcggt atacacgaga cattgaacta tgctcgtgat tagcctgaac
     7741 acggcttctt ttaccacgtg catccatgta gcgtcggacg tgttctggtt taatggcctt
     7801 tgcttctgca tcgccgaaaa ccgctaacaa gtatttttca tgtgccaggt aatctttttg
     7861 tgtcctgggg gcaaggtctg catagtcagc actatttaaa aattttttcc acaattgttg
     7921 aaaggtgagt agtttttttc gaccttcaac gactttctcg taagctaacc aaacctccgc
     7981 tttagaagcg tttgctgggg ctagattctc ggtagtacct cctggcttcc agtagtaacc
     8041 ggaagggcgg aaaaacacac ccttcggcat ccactcattc agcatcaatc acatcttggg
     8101 atacagacgc ttggataata tctgcttcgc tatagtcgcc ccgcataacc agaaaacgag
     8161 cctcatcgga gcagaggtaa tcaactgccc catcaaattt ccccttgctg gttggtttgg
     8221 ttacgttgct catgctgtcc accattcaat aaacatgcag ataccaacgg ttactacggc
     8281 aatcagcacc cagcagatca catctaacag ggcggcgagc cgacgcaggg tgtatttgct
     8341 gtaattctca ggttcaaaat tcattgcgcc tccccaagca cccagcgcag agcctcggca
     8401 tattcgccac tggcatcttc gagtgctttt gtaatttctt tgcgggtttt caggcgcggc
     8461 tttgcatcac cgaggatctg acgctgacgc cgagcttttt catggcccgt ggtgccggcg
     8521 gtcgctgtct cgatctgctt gaccttctcc cgttgctctt cgggtttaag cgatgccaac
     8581 tgacgcgcct gggtaacagt gaccgttccg gactccactg catcgcgaac agcttgggtg
     8641 gcatccagca gtgacagagt tgcgcgtacg gtctggacac tcacgccaaa catcagcgct
     8701 aaatcatcct catcgtgccc gcgctccagc gcatcagcca ttttctttgc tcggcctagc
     8761 ggcgtatctg cctggcggat ttcgttagca cttaccatcg cctgcgccat gcgaatggca
     8821 gagccacgtt tagggactcc cggaaccagt aacggttctt tgcccaattt tgacagacgc
     8881 ttattggctt ccagtgtatg gcgcacacgc tggcgaccat caaccacaca agacagccct
     8941 gtttccgggt ctttccagac gataatcggc tcaagaacgc cctggtccat gatgttcagc
     9001 accattgcct cgctgatagg caggtggata cgctcatcgt aaagcgggtg cgttttgtcg
     9061 gtaaccaggt gcagtttttc aggttcaaac gctaaaacgt tcgttttgcc actcgcgcca
     9121 tataccagct ttgagtcttt agccatcaga gagcctccac gttacggaag ctggtggggc
     9181 aaattgcctt caaatcgcgc atagcctcta gaacatgcat atttgtgcga ttcttggtgt
     9241 gtcgctcggt caggcgatca cactctttcg cccatgattt gacctctgca agaagggcgt
     9301 cacgttcggt gcgcgtctgg cgcagagcta cattcgaaac atcgaggacg gtagccagtt
     9361 ccttgatgat tgctgcctgt tctggtggca tagttttggc tatttcgtac gcctgtttaa
     9421 tcagttgttt tgctgtctta gccatctttt gttctccatc tgacgctctg caacgcgtaa
     9481 atttagggtg cagcaaccca acccatgaga atgggtgaat agctggttaa aatttcttgc
     9541 tgatggggga ccgccactgc aatggcggta cgttagttct ccacacaaca cagaagagca
     9601 cctgcggccg caaatccgct cgggcggatt gtgttagggc acgtcactcg gtggtgctct
     9661 gatgtctttt gtaaaagggc gaaccagaaa caatggggaa actggtgccg ccaagatgac
     9721 atagtccatc agactcactt gatgttaggt tatgcctaat gtcatgtcaa taggcttagc
     9781 ctaatgttgg tcggcggtca aaaaaaatcc cgcataagcg ggatttgtgt gaaataaag
//
LOCUS       DACAPB010000168         9825 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.168, whole genome shotgun sequence.
ACCESSION   DACAPB010000168 DACAPB010000000
VERSION     DACAPB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.168"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(4..894)
                     /gene="pstA"
                     /locus_tag="IT904_003732"
     CDS             complement(4..894)
                     /gene="pstA"
                     /locus_tag="IT904_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP3193455.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(894..1853)
                     /gene="pstC"
                     /locus_tag="IT904_003733"
     CDS             complement(894..1853)
                     /gene="pstC"
                     /locus_tag="IT904_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAP3193456.1"
                     /translation="MAVTKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(1939..2979)
                     /gene="pstS"
                     /locus_tag="IT904_003734"
     CDS             complement(1939..2979)
                     /gene="pstS"
                     /locus_tag="IT904_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP3193457.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(3293..5122)
                     /gene="glmS"
                     /locus_tag="IT904_003735"
     CDS             complement(3293..5122)
                     /gene="glmS"
                     /locus_tag="IT904_003735"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP3193458.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(5284..6654)
                     /gene="glmU"
                     /locus_tag="IT904_003736"
     CDS             complement(5284..6654)
                     /gene="glmU"
                     /locus_tag="IT904_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP3193459.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(7007..7426)
                     /gene="atpC"
                     /locus_tag="IT904_003737"
     CDS             complement(7007..7426)
                     /gene="atpC"
                     /locus_tag="IT904_003737"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP3193460.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(7447..8829)
                     /gene="atpD"
                     /locus_tag="IT904_003738"
     CDS             complement(7447..8829)
                     /gene="atpD"
                     /locus_tag="IT904_003738"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP3193461.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(8856..9719)
                     /gene="atpG"
                     /locus_tag="IT904_003739"
     CDS             complement(8856..9719)
                     /gene="atpG"
                     /locus_tag="IT904_003739"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP3193462.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
BASE COUNT         2332 a         2692 c         2473 g         2328 t
ORIGIN      
        1 atatcaaccg tgtttattct tcgcaaaaac aacgcgcgcc agaatgttca gcagcagtac
       61 gcacagggta atgatcaata ccccggccca ggccaattgc tgccattccg caaacgggct
      121 catcgcaaac ttaaagatcg tcaccggcag gttggcgatc ggctgcatca tgtccgtgct
      181 ccagaactgg ttggagagcg cggtaaacag cagcggcgcg gtttcaccag caatacgggc
      241 aatcgccagc aggataccgg tcataatccc ggacaccgac gctttcagcg taatcgcaga
      301 gatcatcttc cacttcggtg tacccagcgc ataagccgct tcacgcaggc tgtccggcac
      361 cagtttcagc atgttctcgg tggtgcggat aacaatcggc acctgcaaca acgccagggc
      421 aatcacgccc gcccagccgg agaagtgctc catctgcgcc accacaatgg tgtaaacaaa
      481 cagaccaacc acaatcgacg gcgcagagag cagaatgtcg ttaatgaagc gaatcacttc
      541 tgccagccag gatttacgac catattccgc cagataaatc cccgccataa tgcccagcgg
      601 cgtaccgaat accgtggccc acaaaattaa caggccgcta cccgccaggg cgttcgccag
      661 accaccacct tccgtattgg gcggcggcgt catttcagtg aacagcgcca gcgacatacc
      721 atcgataccg cgagtgatgg tggacattaa aatccagatc agccagaaca gaccgaaggc
      781 catcgtcgcc atcgagagcg tcagcgcaat acggtttttg aggcggcgac gcgcctgcat
      841 tttgcggcga gattcagcca gcgccgcagt ggtttgcatt tcaaccatag ccattagcgt
      901 gccccctcat tcttagccag gcgcataatc ataaacttcg atgcggcgag gacgatgaag
      961 gtaatcacaa acaggatcag gcccagttcc atcagtgcgg caacgtgcag accggattcc
     1021 gcttccgcaa attcgttcgc cagcgcagag gtgatactgt tgcccggcat atacagcgag
     1081 gcgctgtcga gctggtaggt gttaccgatg ataaaggtca ccgccatggt ttcaccgagc
     1141 gcgcggccca gccccagcat gatgccgccg ataacaccat ttttggtgaa cggaagaacg
     1201 atacgccaga taacttccca ggtggtgcag ccaataccgt aggccgactc tttcatcatc
     1261 accggggttt gttcgaacac atcacgcatt accgccgcaa tgtacggaat aatcatgatg
     1321 gcgaggatca cgcctgccgc gaggataccg ataccaaatg cggggccaga gaacagcgcg
     1381 ccaacaatcg ggatattcga catgatattg ccgaccggct cctgaaagta aacggcgaac
     1441 agcggcgcaa agataaacag gccccacatg ccgtaaacga tacttggaat ggctgccagc
     1501 agctcaatgg cgatacccag cgggcgtttc agccagccgg gcgcaagctc agtcaggaac
     1561 agggcgatac cgaaactcac cgggacggcg atcagcagcg cgataaacga agtcaccagc
     1621 gtaccgtaga tcggcaccaa cgccccgtag atatcgttcg gtgcatccca ctctttggtc
     1681 cacaggaaag ccagaccaaa tttctgaatg ctcggccagg aggagatgat cagagagaca
     1741 ataatgccac ccaacatcaa tagcacaatc agcgccgcca gttttaccag cacgctgaaa
     1801 attatgtcgc cctttttacc cggtgggtta aaagcaggct tggttacagc cataagttac
     1861 tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc ggataagccg ttaatgcctt
     1921 atccggcctg gagttttatt agtacagcgg cttaccgcta ctgtctttaa tattggtctt
     1981 ccacgcagcg cgaacctgtt caacgacgct atccggcagg ctggcataat ccaggtcgtt
     2041 cgcctgttta gccccggttt tgtacgccca gtcgaagaac ttcagcactt ctgtgccttg
     2101 ttctggtttc ttctgatctt tgtggatcag aatgaacgtg gtagaggtaa taggccatgc
     2161 atcttcgcct ttctggttgg tcagatcctg agcgaaggtt ttgctccagt ctgcaccttt
     2221 tgctgcatta gcgaagtttt cttcggtcgg actaaccggt ttaccatcag cggagatcag
     2281 tttggtgtac gccaggttgt tctgcttagc gtaagcatat tcaacgtaac caattgcacc
     2341 cggcagacgc tgaacgaacg cggcgatacc gtcattacct ttaccgccca gaccgatcgg
     2401 ccattttacg gtagagccag taccaacgtt gtttttccac tcttcgttca ctttcgccag
     2461 gtagctggtg aagacgaagg aagtcccgga gccatctgcg cggcgtacta cagcaatgtt
     2521 ctgtgaaggc agtttcagac ccggattcag tttggcgatg gcttcatcat cccacttctt
     2581 gattttgccc aggtagatgt cgccgagggt tttaccatcc agcaccagtt cgccagactt
     2641 cagccctgga atgttaacag ccagcaccac gccgccaatc acggtcggga actggaacag
     2701 gccttcctga gccaattttt catcagacag cggcgcgtca gaggcaccaa aatcaacggt
     2761 attagcgata atctgtttta cgccaccgga agaacctata ccctggtagt taactttatt
     2821 accggtttct ttctggtaag tgtcagccca tttggcatac accggcgcag ggaaggttgc
     2881 acctgcacct gtcaggcttg cttctgcaaa cacagagaaa gcgctcatcg ataaggtcgc
     2941 ggcgacaaca gttgcgacgg tggtacgcat aactttcata atgtctcctg ggaggattca
     3001 taaagcattg tttgttggct acgagaagca aaataggaca aacaggtgac agttatatgt
     3061 aaggaatatg acagttttat gacagagaga taaagtcttc agtctgattt aaataagcgt
     3121 tgatattcag tcaattacaa acattaataa cgaagagatg acagaaaaat tttcattctg
     3181 tgacagagaa aaagtagccg aagatgacgg tttgtcacat ggagttggca ggatgtttga
     3241 ttaaaaacat aacaggaaga aaaatgcccc gcttacgcag ggcatccatt tattactcaa
     3301 ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc ggtacctttg atcagcgcga
     3361 cgtgataagc cagtagctgc agcggaacgg tgtagaagat tggtgcaatc acctcttcca
     3421 catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg
     3481 cgaagacata caactgaccg ccacgcgcgc gaacttcttc aatgttggat tttagttttt
     3541 ccagcaattc gttgttcggt gcaacgacga taaccggcat atcggcatca atcagcgcca
     3601 gcggaccgtg tttcagttca cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt
     3661 tcagcttcaa tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg
     3721 cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc tgagacagca
     3781 tctgctcaat acggctcggc aacgcctgca gaccatgcac aatgtcatgt tcaatggagg
     3841 catccagacc tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag
     3901 tggtgaatgc tttagtggat gccacgccga tttctgtacc cgcgttggtc attagcgcca
     3961 gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagt gaaccaaggt
     4021 aacccagctc tttcgacaga cgcaggccag ccagggtatc cgcggtttcg ccagactgtg
     4081 acaaggtgat catcaggctg ttacgacgca cggcagattt gcgatagcgg aattcagagg
     4141 cgatttcgac gtcgcacgga atacctgcta gcgattcaaa ccagtagcgg gaaaccatac
     4201 cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt
     4261 cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgtccggtaa
     4321 gggtgttttt gatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa
     4381 tgcctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg
     4441 cgccagtttt atcgaagatg tttaccgaac ggcgagtgat ttccgcaata tcgccctctt
     4501 caaggaagat aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt
     4561 tttcgcccat ccccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat
     4621 ccgggtgacg ggagtccatg atcactgtac cgtacgcacc acgcagctgc gggatagcac
     4681 gcagaacggc ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa
     4741 tcacttcggt gtcggtttca gaaacgaagg tatagccacg cgcttttagc tcttcacgca
     4801 gcggttcatg gttttcgatg atgccgttat gcaccaccac aatgtgttca gaaacatgcg
     4861 gatgcgcatt cacttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccag
     4921 tgccgccatg cagaggatgt tcttccgctg cctgtgccag catctggact ttaccgaggc
     4981 gacgcaggcg ggtcatatga ccttctgcat caacaacggc cagaccggca gagtcatatc
     5041 cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga
     5101 tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct
     5161 gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta
     5221 ctgcttgtgg gagggagatt atgttatctc ctcatcccat gtgaccgggt tagccggcca
     5281 gaatcacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct
     5341 gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc
     5401 gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccaacaa acacatcgtc
     5461 gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc
     5521 gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgac cagctttcga
     5581 gcctttaccc agacgcgctt ttttcatctc aacgaagtta ccgacgtgag caccttccag
     5641 caactcagca ccaggacgca aacgggcaaa cgggccaatg gtacaggccg ctgccagatt
     5701 cgcatcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt
     5761 aatcacgcaa ccggtgccaa ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat
     5821 aacgttagta tcaatttcaa catcgcgccc gtgagttagc gtaccacgca gatcaaaacg
     5881 cgctggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactgata
     5941 aacacgctcc agacgggaga gttgcaggcg gttattcacg ccttctactt cgcttaaacg
     6001 ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt
     6061 gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc
     6121 tgcgccgttg gcaatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt
     6181 ggcatctttg tgctcaacaa tgccggtaac tttgccgttt tcacgggtga tacgtccata
     6241 accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc
     6301 acgcagacgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat
     6361 gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg
     6421 ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttta gcagatcgcc
     6481 gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac
     6541 atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat
     6601 gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt
     6661 cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt
     6721 catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagc
     6781 gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttaattt
     6841 aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaatagg aaaacagggg
     6901 ggttttatat cagcaggatc tatgtgaacg ctattcagga cgggtcacac gcgcaaaaaa
     6961 aagccagcct gtttccagac tggcttttgt gcttttcaag ccggtgttac atcgcttttt
     7021 tggtcaactc gataacgcgc agctgcgcga tcgctttggc cagttccgca gacgcctgag
     7081 cgtaatctac gtcgccgtga gagctgctaa tgtgctcttc agccttacgt ttcgcttcca
     7141 tggctcgcgc ttcgtcgaga tcctggccgc gaattgcggt gtcggccaga acggtcacgt
     7201 tgccaggctg cacttcaaga atgccgccag acagatagat aaactcttcg tgaccgtgct
     7261 gtttcacgat gcgaatcata ccaggcttaa tggcggtgag cagcggtgcg tggccagggt
     7321 agatccccag ttcaccttcg ctacccgtta cctggatttt ctcgaccaga ccagagaaca
     7381 tttgttgctc tgcgctgacg acgtccaggt ggtaagtcat tgccatatca ccctccgatt
     7441 aaggcgttaa agttttttgg ctttttccac agcttcttcg atggaaccga ccatgtagaa
     7501 cgcctgctcc ggcaggtgat cgtattcgcc ttccatgatg cctttaaagc cacggatggt
     7561 gtctttcagg gagacgtatt tacccggaga accggtgaat acttctgcca cgaagaacgg
     7621 ctgggacagg aagcgctgga tcttacgagc acgcgctacc accagtttgt cttcttcaga
     7681 cagttcatcc atacccagga tggcgatgat gtctttcagt tcctgataac gttgcaggat
     7741 ggactgaacg ccacgcgcgg tgtcgtagtg ttcctgacca accaccagcg ggtccagctg
     7801 acggctggtg gagtccagcg ggtcaacggc cgggtagata cccagagacg cgatctgacg
     7861 gctcagtacc acggttgcgt caaggtgcgc aaaggtggtt gccggagacg ggtcagtcaa
     7921 gtcatccgca ggtacgtata ctgcctgtac ggaggtgata gaaccagttt tggtggaggt
     7981 gatacgttcc tgcagaacgc ccatctcttc cgccagggtc ggctgataac ctaccgctga
     8041 aggcatacgg cccagcagtg cggatacttc cgtaccggcc agggtgtaac gatagatgtt
     8101 gtcaacgaac agcagaacgt cacgaccttc gtcacggaat ttctcagcca tggtcagacc
     8161 ggtcagagca acgcgcagac ggtttcccgg cggctcgttc atctggccat acaccaggga
     8221 tactttgtcg ataacgttgg agtcggtcat ttcgtggtag aagtcgttac cctcacgagt
     8281 acgttcacct acgcccgcaa acacagagta accggagtgc tcgatcgcga tgttacgaat
     8341 gagctccatc atgtttacgg ttttacctac acccgcacca ccgaacagac caactttacc
     8401 gcccttagcg aacggacaca tcaggtcgat aactttgata ccggtttcca gcagttcctg
     8461 agagtttgac agctcttcgt aggaaggtgc tgcgcggtga atcgcccaac gctcttcttc
     8521 accgatctcg cctttcatgt cgaccggttc acccagtacg ttcatgatac ggcccagagt
     8581 cgctttacct accgggactt caatcgggtg ttcgaggtct tttacatcca gaccgcgacg
     8641 cagaccgtcg gaggaaccca ttgcgatggt acgtacgata ccgccgccga gctgctgctg
     8701 aacttccagc accagacgct cattaccatt ttgcacctca agagcatcgt acacgcgcgg
     8761 tacggcatcc tgagggaatt cgacgtcaac tacggcgccg attacctgga caatctttcc
     8821 agtagccatc ttaaatcctc tacgaaataa cctgtttaaa ccgcggcggc ccccgagacg
     8881 atctcggtga gttcctgagt aatgctggcc tgacgagctt tgttgtatac caactgcagc
     8941 tctttaatca ggctgccgcc attgtcggtc gcggctttca tcgccaccat acgggcggcc
     9001 tgctcgctgg ccaggttttc aaccacgccc tgataaacct gagattcgac ataacgacgc
     9061 agcagggtat ccagcaacgc cttcggatcg ggttcgtaca ggtaatccca ggatttatgt
     9121 ttcagatcat catcatctga tgccggtaac ggcagcagct ggctgatggt cggaacctga
     9181 gacatggtgt taataaattt gttgctgaca atgtaaagct tgtccagacg gccttcgtcg
     9241 taggcctgca acatcacttt taccggaccg atcagttcgg acagggaagg gttatccccc
     9301 atgccggtga cctgggcaac aacattgccg cccacggagt tgaagaacga cacgcctttc
     9361 gagccgatca ttgcgaggtc gcattgaacg cctttgtcgg tccaggtctt catttccgcc
     9421 agcagttttt tgaacaggtt aatgttcaaa ccaccgcaca aaccacggtc ggtcgacacc
     9481 accaggtagc ccacgcgttt aacgtcgcgg tcttccaggt aagggtgctt atattccaga
     9541 ttaccgtgtg caaggtgacc aatcactttg cgcatggttt ctgcataagg acggctggcc
     9601 gccatgcgat cctgcgattt acgcattttg gaagcggcga ccatctccat cgctttagtg
     9661 atcttttgcg tgttctggac gcttgcgatc ttactacgta tctcttttgc gccggccatg
     9721 agcttctcct caatgccttg cggcctgccc taaggcaagc cgccagacgt taccaggatt
     9781 gggttgcttt gaaggaatcg aggatgcctt tcagcttgcc ttcga
//
LOCUS       DACAPB010000169         9648 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.169, whole genome shotgun sequence.
ACCESSION   DACAPB010000169 DACAPB010000000
VERSION     DACAPB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.169"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..552
                     /gene="hlyE"
                     /locus_tag="IT904_003740"
                     /pseudo
     CDS             1..552
                     /gene="hlyE"
                     /locus_tag="IT904_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(759..1220)
                     /locus_tag="IT904_003741"
     CDS             complement(759..1220)
                     /locus_tag="IT904_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAP3193463.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1297..1956)
                     /locus_tag="IT904_003742"
     CDS             complement(1297..1956)
                     /locus_tag="IT904_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAP3193464.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2028..2321)
                     /locus_tag="IT904_003743"
     CDS             complement(2028..2321)
                     /locus_tag="IT904_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193465.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2563..2964
                     /locus_tag="IT904_003744"
     CDS             2563..2964
                     /locus_tag="IT904_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAP3193466.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHEQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3067..3435)
                     /locus_tag="IT904_003745"
     CDS             complement(3067..3435)
                     /locus_tag="IT904_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193467.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPALTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            3955..4650
                     /gene="minC"
                     /locus_tag="IT904_003746"
     CDS             3955..4650
                     /gene="minC"
                     /locus_tag="IT904_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAP3193468.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPTPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4674..5486
                     /gene="minD"
                     /locus_tag="IT904_003747"
     CDS             4674..5486
                     /gene="minD"
                     /locus_tag="IT904_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAP3193469.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5490..5756
                     /gene="minE"
                     /locus_tag="IT904_003748"
     CDS             5490..5756
                     /gene="minE"
                     /locus_tag="IT904_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAP3193470.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6128..6471)
                     /locus_tag="IT904_003749"
                     /pseudo
     CDS             complement(6128..6471)
                     /locus_tag="IT904_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6506..6625)
                     /locus_tag="IT904_003750"
     CDS             complement(6506..6625)
                     /locus_tag="IT904_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP3193471.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6586..6771)
                     /locus_tag="IT904_003751"
     CDS             complement(6586..6771)
                     /locus_tag="IT904_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193472.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYQKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            6872..7045
                     /locus_tag="IT904_003752"
     CDS             6872..7045
                     /locus_tag="IT904_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193473.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7107..7391
                     /locus_tag="IT904_003753"
     CDS             7107..7391
                     /locus_tag="IT904_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAP3193474.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            7395..7730
                     /locus_tag="IT904_003754"
     CDS             7395..7730
                     /locus_tag="IT904_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAP3193475.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(7787..9435)
                     /locus_tag="IT904_003755"
                     /pseudo
     CDS             complement(7787..9435)
                     /locus_tag="IT904_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322580.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9465..9648)
                     /locus_tag="IT904_003756"
                     /pseudo
     CDS             complement(9465..9648)
                     /locus_tag="IT904_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422011.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT         2681 a         2280 c         2127 g         2560 t
ORIGIN      
        1 ttaaggtact ggatgacggc atcacgaagc tgaatgaagc gcaaaaatcc ctgctggtaa
       61 gctcacaaag tttcaacaac gcttccggga aactgctggc gttagatagc cagttaacca
      121 atgatttttc agaaaaaaaa cagctatttc cagtcacagg tagataaaat caggaaggaa
      181 gcgtatgccg gtgtcgtcgc cggtccattt ggattaatca tttcctattc tattgctgcg
      241 ggcgtagttg aagggaaact gattccagaa ttgaagaaca agttaaaatc tgtgcagaat
      301 ttctttacca ccctgtcaaa cacggttaaa caagcgaata aagatatcga tgccgccaaa
      361 ttgaaattaa ccaccgaaat agtcgccatc ggggagataa aaacggaaac tgaaacaacc
      421 agattctatg ttgattatga tgatttaatg ctttctttgc taaaagaagc ggccaaaaaa
      481 atgattaaca cctgtaatga gtatcagaaa agacacggta aaaagacact ctttgaggta
      541 cctgaagtct gataagctat tattctctcc atgtactcaa ggtataaggt ttatcacatt
      601 taaaaacaca gacgtttttt aaagattgag agttacctgt tgataataag gtatcgggta
      661 actctcatta tgacagagcg ctttcaaaaa cagggctacg aaatggcgag catccagacg
      721 tatattaaga aagcccccat caacatctat taaatttatc actgcgccca gtcaggttta
      781 tttaagatat gatcctgcca gtcaatcact tctgattctt tcactgcgat atgacgcaca
      841 gagatacgtt caccatgcat tgccgctttc gaacccgtaa gtagcggatg ccacgcaggt
      901 aaatctttac cttccgccag caaccgataa gcgcaggtca ttggcagcca ttcgaatgtt
      961 ggcagatttt cacgggttaa tttaatgcag tcgggttcaa actcgaaacg acgttcgtag
     1021 ttccgacatt gacaggtttt aatattgagc tggcgacagg cgacgttagt gaagtagatt
     1081 tcgtcggtgt cttcatccat cagtttatgc aggcaacact gaccgcaacc atcacacaac
     1141 gactcccatt ccgcatcgct catttcgtcc agggttttac tttgccagaa aggtacatcg
     1201 ctcatcaggt gttccgccat tacgttaaaa cgcaccttat aaccagtctg gcacagcgat
     1261 gcaagttttg ccgccgcttt caggcggcaa aaagtattac aaaacgcgag ttgtcaaaga
     1321 atgcccatcg aaagtgactg tcagctcatc accgctttgc aacgggccga cgccatcagg
     1381 cgtgcctgtc aacacaacgt caccggcctt gagggtaaaa aacttgctca tataagcgat
     1441 cagcggaacg attttatgga tcatgtccgc agtcgtacct tgctggcgtt gctcgccgtt
     1501 tacgctcagg cccagcgttg tattttgcgg gtcgccggta aattccgccg cgggaataaa
     1561 cccggaaagc ggacaagagt tatcaaacgc tttagccttt tcccacggct gcccggcttt
     1621 cttcattttt ccctgaacat cacgcaacgt cagatcgagc gccacgccat aaccggcaat
     1681 ggctttgcgg acatgctctt ccgtagcctg acgcagcgtc gcgccaatca acaccgccag
     1741 ttcgacttca tgatgaactg aaccgaaatc ggatgggatc gccaatggct gccgcagatc
     1801 gcacagtgcc gtttctggtt taataaacag cactggctct tcgggcactg cgctgcccat
     1861 ctctttaata tgtttggcat agttactgcc aacacagact actttactca ccggataatc
     1921 cagcagcgca ccttgccagt tgtgatgttg atacataacg ttcccctcac cttatcgtgg
     1981 atatgaaaac ccgcgccgac aaagataatg ccaccgccgg atatcggtta tttgttagtg
     2041 tcgtctgttt tctgccccat gacggaaaga tgttgcttca gcaaatcttc gggtggtggc
     2101 ggtaactgca aatagtaacc ttgctcggtt aatgccagtt taaccttttc aatatcagca
     2161 ttgaccagtt tcttccgccc atccaacggc agaatcatcg ctaactgagg ctgaccaaaa
     2221 cctttcatca gttcctcagg aacacgcgaa aaatcgtctt ttttttcgac atataaatag
     2281 gtctggtcac gcttgctgct tcgataaatc acacaaaaca tacttttact ctttaagatg
     2341 ccaggttttg gcataagcta acatcaatga aacccgcgcc acaagccagc cagctgtaga
     2401 tttctgcggt ttgttgagtc catgcccctt tataagtaat ccccccggca aaaaatgccg
     2461 cgccgggatt ttaccgaacg cagaaaagcc actggaattc tcccgtaagg gattgttata
     2521 ttataacagt tcatcgtact cattctgaac aggagactac caatgaaaat caagagcatc
     2581 agtaaggctg tattgctgtt tgcactgcta acctccacga gctttgctgc gggtaagaat
     2641 gtcaatgttg agttcagaaa aggccatagc agcgctcagt actccggcga aataaaggga
     2701 tacgattacg atacatatac cttctatgcc aaaaaaggtc agaaagtgca tgtgagtatc
     2761 tctaatgaag gcgctgatac ttacctcttc gggccaggaa tcgatgattc tgttgattta
     2821 tccagatatt ccccagaact cgatagtcac gaacaatact cactccctgc ttctggcaaa
     2881 tatgagctgc gagtgctgca aacccgaaac gacgcccgta aaaacaaaac taaaaaatat
     2941 aacgtcgata ttcaaattaa gtaaatgtca accgacgggg actctccccg tcataaacgt
     3001 tgacgcccca ctcctctctg tcgctcaaga ggttaatgac gaatagaaca taatgtaaac
     3061 aagacatcac tcacgacaca taaataacat ctttgtggtg atttgattga tttttccact
     3121 ccgcttacca tccacaccaa aataacgatc atcacgacat aatttttctt taacatcgca
     3181 aaatacaccg ttagcgaatg taaattcgct gggatcgtag ccttgtaatg attgtaagtt
     3241 ttcagcggct ttttcgccga gatatttcct tgttaacgct ggggaaatgc catctgagtt
     3301 cacacaaata tacgggtcgc aaacaacgcc gcacgccgga gattgaagtc cgccagcgtc
     3361 atttccagac gccattgatg agaacatcat ccccacacct gacaatccga tataaaaaat
     3421 acgcatcata ttcatagcac atcctgataa tccaaccgta caacacttat gctcatttca
     3481 tcatcaatcc gtcatcaaaa ccgctgacct ggacgagtta cgatgacctg acagaagtat
     3541 cagacgtctt ctgcggtttc tatgatagag caattctgtg gcggatatta agataacggg
     3601 tatctgttct ttaatgatgc cgcaaaaaat ggaaagttaa gaaagcctgg cgtggtgaaa
     3661 aaatacactt gcctcaatac tggatattta aacaggtctg gagatgctgt cacgattagg
     3721 gtgcggaaaa aggaagcgta aataagaaca atcaaaagta cgacggcaat gggttgattg
     3781 acaagggtat tttttaagct atgaatcagc gccatttatc acagaataga cttttactct
     3841 gaataaatgg gagggtgact tgcctcaata taatccagac tataacatgc cttatagtct
     3901 tcggaacatc atcgcgcgct ggcgatgatt aatagctaat tgagtaaggc caggatgtca
     3961 aacacgccaa tcgagcttaa aggcagtagc ttcactttat ctgtggttca tctgcatgag
     4021 gcagaaccta aggttatcca tcaggcgctg gaagacaaaa tcgctcaggc ccccgcattt
     4081 ttaaaacatg cccccgttgt actcaacgtc agtgcactgg aagacccggt aaactggtca
     4141 gcgatgcata aggcggtttc ggcaaccggt ttgcgggtta ttggcgtaag tggctgcaaa
     4201 gatgcgcaac ttaaagccga aattgaaaag atggggctgc ctatcctgac ggaaggaaag
     4261 gaaaaagcgc cacgtccagc tcccacaccg caggctccag cgcaaaatac aacgccggtc
     4321 acaaaaacgc gtttaataga taccccggtg cgttccggtc agcgtattta tgctccacaa
     4381 tgtgatctga ttgttacaag ccacgttagc gctggggccg aattgattgc cgatgggaac
     4441 attcatgtct atggcatgat gcgcggtcgt gcgctggcag gggcaagtgg tgaccgggaa
     4501 acgcaaatat tttgtacgaa cctgatggcg gaactggtgt ccatcgcagg tgaatactgg
     4561 ctgagtgatc aaatcccagc agaattttat ggcaaagcgg cgcgactgca gttagtcgaa
     4621 aacgctttga ccgttcaacc gttaaattga tcccttttta acaaggaatt tctatggcac
     4681 gcattattgt tgttacttcg ggcaaagggg gtgttggtaa gacaacctcc agcgcggcca
     4741 tcgccactgg tttggcccag aagggaaaga aaactgtcgt gatagatttt gatatcggcc
     4801 tgcgtaatct cgacctgatt atgggttgtg aacgccgggt cgtttacgat ttcgtcaacg
     4861 tcattcaggg cgatgcaacg ctaaatcagg cgttaattaa agataagcgt actgaaaatc
     4921 tctatattct gccggcatcg caaacacgcg ataaagatgc cctcacccgt gaaggggtcg
     4981 ccaaagttct tgatgatctg aaagcgatgg attttgaatt tatcgtttgt gactccccgg
     5041 cagggattga aaccggtgcg ttaatggcac tctattttgc agacgaagcc attattacca
     5101 ccaacccgga agtctcctca gtacgcgact ctgaccgtat tttaggcatt ctggcgtcga
     5161 aatcacgccg cgcagaaaat ggcgaagagc ctattaaaga gcacctgctg ttaacgcgct
     5221 ataacccagg ccgcgtaagc agaggtgaca tgctgagcat ggaagatgtg ctggagatcc
     5281 tgcgcatcaa actcgtcggc gtgatcccag aggatcaatc agtattgcgc gcctctaacc
     5341 agggtgaacc ggtcattctc gacattaacg ccgatgcggg taaagcctac gcagataccg
     5401 tagaacgtct gttgggagaa gaacgtcctt tccgcttcat tgaagaagag aagaaaggct
     5461 tcctcaaacg cttgttcgga ggataagtta tggcattact cgatttcttt ctctcgcgga
     5521 agaaaaacac agccaacatt gcaaaagaac ggctgcagat tattgttgct gaacgccgtc
     5581 gcagcgatgc agaaccgcat tatctgccgc agttgcgtaa agatattctt gaggtcattt
     5641 gtaaatacgt acaaattgat cctgagatgg taaccgtaca gcttgagcaa aaagatggcg
     5701 atatttctat tcttgagctg aacgtgacct taccggaagc agaagagctg aaataagccc
     5761 gctgtaaaag cgcatttatc ttcaaggcag agttatctct gccttgagtt tttcatccct
     5821 ctcatccacg ttgtggtaaa gcggcgagta ttcttgctga tactcctcat tgctatttcc
     5881 acctccccct ttacacctta aggctgttcc cacgcacaaa acaaaatgtt tatgcttggt
     5941 tgagtaaata accttattgt tagtgtggat atactgggag atgataaccc cgcaatttca
     6001 ttattcattg ttctcccata ggatgaggtt tcccgccgta actggcgggc tttttttgcc
     6061 ccagaatttt ccctttcaac atcctgtaaa cgaaaactgc gccgaagcgc agtttaatcg
     6121 ttgatggtca gaacgaccag ttcacaccag ccaccgcgtt ccacggggat tccacaccgg
     6181 caccatggct ataccccacc ccgagatgtc cgctaaacgt actgctgaat gaggctttaa
     6241 tagctgcctg gtatattcca cgtctgcccg acaaatcatt gacgaaatta ccgtcattat
     6301 tcaccttcac ccggttatca tcgacaaatt ctttgcgcac agccgccttc agccagggtt
     6361 catttccata ccgttcccca ggcgcatgtt gtaactcagc gttgcaccca gttcacgata
     6421 tatactgcgg gtatcgactg atttcgattc catgccattg gataaatgat agtagcgtct
     6481 aagtgaccta agctctgctg caacatcagc ccgcaggcac cagaccaggg gattcatcct
     6541 gaagagacag cgcaggtgta ttgtgttcac cgctcaccaa agacatcatg atgaaatgat
     6601 gatatcccgc ataagaaaga ggcatttttt acacgcagtg cgctgaagtg tagttggata
     6661 aaaaatcact cttttctgat aatactgtcg tacgcttatc tttcgacaat gaggccgtcg
     6721 gcaaaataaa atgactaatg taatcgttgc tgatgaatat cttctgctca cataaaaatc
     6781 acacaataat attgataaat cagattatct tacctttaca acattgcgtt aacagtcaat
     6841 gacacttctt gaataaaaat attgtcaatc aatgagttat tcctcattta agattattct
     6901 cattcacgta aaaaacataa ttcctatcat cacagccacg ctcattttga attatctgaa
     6961 taatagcgag cgttctttag ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc
     7021 aacttactta ataccggaca tctaagttga agaaaaaaat acttgccttc gggcttatct
     7081 cagcattgtt ctgctccacg ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat
     7141 tgctaggcgc aggcgttggt ttacttactg gcaatggtgt taatggcgta ctgaaaggtg
     7201 cagctgttgg cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcgc
     7261 gtaaaggtgc aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg
     7321 gcctcgaagg ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta
     7381 aaatgaaata aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg
     7441 caccaatgac ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata
     7501 ttacttgcgc agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg
     7561 ttgcggtaat gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg
     7621 aactgagcgc caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc
     7681 tgttgatcac ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttcatat
     7741 gtaccgcgtc gatgaatacg acgcggtttt ttagctaacg gcgggatcag aacttatatt
     7801 tcaggcccac catcactgcg gtgtcattgt agccattatc acccagctgc acgcccacat
     7861 tccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa
     7921 attactcgcc ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact
     7981 gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt
     8041 tttctcctga gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc
     8101 gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga cgcccatcca
     8161 gatagcctgt gcctgtggct ggaagatcca cgtatatttc gccgcttcca gaccaaagga
     8221 tttattcagg gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt tcgcgctata
     8281 ggattcatag gctaattcat cccctttcac cgagttttta aaccagctat attgcgccca
     8341 ggagtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaggtgg catacagccc
     8401 tgcgctatag cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttaa
     8461 gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac tatcggtaaa
     8521 accaccggtt aacagatcgc cccctaactg cgagacgtag cgatgcgagg tcgttctcaa
     8581 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct
     8641 tgaacgctcc tgttcagtta caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag
     8701 acgcataaca aaaagagtgt tagcagctgc caggttagcg acgtaacttc cagcttcggg
     8761 gcgcaggacg ggggtaggcg tttctgttga ttctgaattt ccctctgagc tgttatcctc
     8821 ctcattcact gactgataac ttgtcaaata ccagtttttg ccttgttgtt ttagttgata
     8881 ttcgaaagct ccagctgcaa tccttgcatc accttatctg aaggcatctg ctggagattc
     8941 atcgccattc acagttatga gttcgatccc gtttagcgtg gccgcgccgc ttcctccagc
     9001 atttttcaca tagacggtag ttgaacccga agtattaccc tcaacaataa gtctgtccgt
     9061 agccgattta tcatcaccta atacggtgcg gatattaagc tgaccattat taccgacata
     9121 attacctttt acggttaatg tttttccttg ggtaaatgtt tgaccatcat catacgaaag
     9181 attgataata ctgtctgaat tagtcagatg ggtaacaacg gagtcatctt tcatattcca
     9241 gattgtattt tctgaaagct tcagcgtggt tgtgccgctg acgagtttag tcgctccatt
     9301 tagaatagag tcggacgaaa ttatgtttaa gtcacctcca gtattattaa ccagaacatt
     9361 attaccaggt gtgataatac ttcgattaag gatacttaca tccgttgtag ttttgttaac
     9421 atcaatacta tccaaagcac ttgaaagttc aacacttaat agtgctattt tggcttgaga
     9481 atcgatattt gatgcatcaa ttgatattcc tttatcttgt tttgataccg cggtgacatt
     9541 ggaaaaaaat aactctccac cattgtatat ttttgcaatg ggggctttag tgcctgttgt
     9601 tgtaatagaa ctatttttta tatcaatgtt accttgtcga atagcaac
//
LOCUS       DACAPB010000170         9617 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.170, whole genome shotgun sequence.
ACCESSION   DACAPB010000170 DACAPB010000000
VERSION     DACAPB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.170"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..157)
                     /locus_tag="IT904_003757"
     CDS             complement(<1..157)
                     /locus_tag="IT904_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAP3193476.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVF"
     gene            complement(242..562)
                     /gene="chbB"
                     /locus_tag="IT904_003758"
     CDS             complement(242..562)
                     /gene="chbB"
                     /locus_tag="IT904_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAP3193477.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(861..1199)
                     /gene="osmE"
                     /locus_tag="IT904_003759"
     CDS             complement(861..1199)
                     /gene="osmE"
                     /locus_tag="IT904_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAP3193478.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1401..2228
                     /gene="nadE"
                     /locus_tag="IT904_003760"
     CDS             1401..2228
                     /gene="nadE"
                     /locus_tag="IT904_003760"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP3193479.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2458..3345
                     /gene="cho"
                     /locus_tag="IT904_003761"
     CDS             2458..3345
                     /gene="cho"
                     /locus_tag="IT904_003761"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAP3193480.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3305..3880)
                     /gene="ves"
                     /locus_tag="IT904_003762"
     CDS             complement(3305..3880)
                     /gene="ves"
                     /locus_tag="IT904_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAP3193481.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPFQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(4083..4568)
                     /gene="spy"
                     /locus_tag="IT904_003763"
     CDS             complement(4083..4568)
                     /gene="spy"
                     /locus_tag="IT904_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAP3193482.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4898..5866)
                     /gene="astE"
                     /locus_tag="IT904_003764"
     CDS             complement(4898..5866)
                     /gene="astE"
                     /locus_tag="IT904_003764"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAP3193483.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFI
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5859..7202)
                     /gene="astB"
                     /locus_tag="IT904_003765"
     CDS             complement(5859..7202)
                     /gene="astB"
                     /locus_tag="IT904_003765"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAP3193484.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHFTVANLNNKFHRSLEALVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7199..8677)
                     /gene="astD"
                     /locus_tag="IT904_003766"
     CDS             complement(7199..8677)
                     /gene="astD"
                     /locus_tag="IT904_003766"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAP3193485.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDAGAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8674..>9617)
                     /gene="astA"
                     /locus_tag="IT904_003767"
     CDS             complement(8674..>9617)
                     /gene="astA"
                     /locus_tag="IT904_003767"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAP3193486.1"
                     /translation="NEATLSARIERAIKTWQGELPKSEQGYVFVLEDSETGTVAGICA
                     IEVAVGLNDPWYNYRVGTLVHASKELNVYNALPTLFLSNDHTGSSELCTLFLDPDWRK
                     EGNGYLLSKSRFMFMAAFRDKFNDKVVAEMRGVIDEHGYSPFWQSLGKRFFSMDFSRA
                     DFLCGTGQKAFIAELMPKHPIYTHFLSQEAQDVIGQVHPQTAPARAVLEKEGFRYRNY
                     IDIFDGGPTLECDIDRVRAIRKSRLVEVAEGQPAQGDFPACLVANENYHHFRVVLVRT
                     DPATERLILTAAQLDALKCHAGDRVRLVRLCAEEKTA"
BASE COUNT         2342 a         2591 c         2359 g         2325 t
ORIGIN      
        1 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
       61 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
      121 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
      181 aatttaaata ttgcggtatt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
      241 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
      301 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
      361 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
      421 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
      481 ctgcctgtgc gcgcattttt gataccagta aagaggtaga catgcccgca gaacaaaaca
      541 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
      601 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
      661 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
      721 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
      781 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
      841 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
      901 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
      961 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     1021 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     1081 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agccgtacaa
     1141 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     1201 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     1261 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     1321 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     1381 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     1441 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     1501 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     1561 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgcctggaa accggcaacg
     1621 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggccgac gaacaagatt
     1681 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     1741 cggtattggc cagcgaacag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     1801 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     1861 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     1921 atggtgacgg cggtacggac attaatccgc tgtatcgtct caacaaacgt cagggtaaac
     1981 agttactggc ggcattagct tgcccggaac acctttataa gaaagcgcca acggccgatc
     2041 tggaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     2101 tcgacgacta tctggaaggg aaaaacgtac ctcaacaggt cgccagaaca atagagaact
     2161 ggtatctgaa aaccgaacat aaacgccgtc cgccaattac cgttttcgat gatttctgga
     2221 aaaagtaata atttgcacat attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     2281 gcggcgtgaa cgccttattc gacctataaa actatgcaaa ttcaatacat tgcaggagtc
     2341 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct
     2401 tttttaatcg cttcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     2461 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     2521 gaacatttac gttcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     2581 ggtgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaacatccg cagccgcgtc
     2641 ctttctcatt tacgtacccc ggatgaagcc gccatgctac ggcaatcccg acggatcagc
     2701 tggatatgta ccgccggtga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     2761 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     2821 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     2881 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagac catcgctgat
     2941 gaacaaaaac tttgttatgg cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt
     3001 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     3061 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     3121 ggggcggtgg cgctgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     3181 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     3241 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     3301 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     3361 gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct
     3421 ggtcggtagt gagcaattta tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     3481 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     3541 catccaggcg tgtcatgatg ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     3601 ttaccacctg gtccgctgca aaggcaaaag gctggaacgg ttttaaggta tggttaaagc
     3661 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     3721 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     3781 aatcacgttt tgccggtggg aacgtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc
     3841 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     3901 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     3961 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     4021 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     4081 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     4141 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     4201 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     4261 gcttcagctt ttactttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     4321 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gcggatctgc
     4381 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     4441 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     4501 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     4561 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     4621 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     4681 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     4741 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     4801 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     4861 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     4921 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     4981 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggaa
     5041 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     5101 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     5161 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     5221 gcaacgcttt gccaagttcc agcgtacagg ccagcgcgcc aaaatgtctg gcgctgaaat
     5281 gggtaaacgt accaccaggt tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     5341 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     5401 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccagcgcaca gattctttgc
     5461 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     5521 tttcagcaaa tagctgccaa cgaccgccaa acattcgatt catatcgcta tggcaataac
     5581 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     5641 ggatctcgcc gtgagatatc gcgccaagca acgcgtccag catctccaca ggtgccgtct
     5701 cattaccgtg tattcccgct gaaatcacca gtgcgccttg cggtggcgtt aatggcgtca
     5761 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     5821 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     5881 cgctggaacg gataaaccga accgagattc agtaattggc tcaatacatc cagtgcttcc
     5941 cgcccttcgc gcagcaattg cgggtcggcc agatcggcag cagtaaggcg atcgcggtag
     6001 taacgatcca cccagtcatt tagcgcatta aacagcgtat cgttcatcat caccgccgga
     6061 ttcaccgccc ggcgttcttc ttctgtcaat accacccgca accgcaggca cgccgggccg
     6121 ccgccattcg ccatgctttc acgtaaatca aagactttta gttcgctaat cgggttgtca
     6181 gcggcaagga gttcattgag ataaccccat actccggcgt gttcccgaca ctcctgaggc
     6241 agcaccaaca tcatggaacc atcatcgcgg ctcagcagtt ggctgttaaa cagataggta
     6301 gacaccgtat cagacacgga aacctgagtt gccggaactt ctatcgccat aaaaccattg
     6361 acccgcgcac gcaggtttgc cagtaactgt gactggcgag cgaacgcctg ttggtggcaa
     6421 aacagcacct ggcggttact cacggcaatc acgtcattat gaaaaacgcc ctggtcgata
     6481 acgtccgggt tttgctgggc gaaaatcacc tgttggggat tcacctgatt cagccttgcc
     6541 accgcctcgc tggcttcgcg agtctgtcgc gccggataac gggaaggccg ggtatcattg
     6601 ccttcttctc gcccgtagac aaaaagttgc atacccggtt caccgtaatg accgccgaga
     6661 cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta cctgtggcaa cgccgaatgg
     6721 acgctaaatt tctcttcgtc gttaaaaatc gcttttaaca gcgattcagt gacgagcgct
     6781 tccagcgaac ggtgaaattt attgttcagg ttggcaaccg tgaaatgcac tttgccatcc
     6841 agcgtatcgg cagatggcgc gatcgttgcc gcattggcta cccacattgg cgaagcggaa
     6901 ctgacgctgg aaagccagtg cggtgcctgg cgtgcgacct tttccagtac ctgctcatcg
     6961 ctaccactga atcccaactg acgcagcacc ggaataaacg gacgctcgtg cggcgggatc
     7021 accgcctggg ggaatcccgc atcggcaagg gttttcattt tcagtaagcc ctgttttgcc
     7081 gcctgtcgcg ggttagacac ctgaaaacgg tgacgggtag aggcttcatt accaaacgac
     7141 aggccagcgt aatgatgcgt cagccctacc agcccgtcga aattaacttc ccaggcgttc
     7201 atcgcaccac ctcatcggaa aaatccagcc cggggttaag cgttgcgggc aaagttaacg
     7261 agtccgactc caggctcgcc ataggccatg cgcagtaatc tgcggcatac caggcgctgg
     7321 gacgatggtt accggatgca ccaatgccgc cgaatggcgc ggtactggca gcaccagtaa
     7381 gcggtttgtt ccagttaaca atccccgccc gcgcctccag caacagttga tcgaattttt
     7441 cccgctcggg ggaaaccaga ccgcaagaga gtccgaagcg agtgttattc gccattagaa
     7501 tcgcttcctc gaaagaatca taacgccaga cgcgcaaaag cggcccgaac acctcttcat
     7561 ctggtacgcc agcaacgcct gtcatttcaa tgatccccgg cgtcagcaac gatgtctctg
     7621 attgtaataa gcgcggcgca agcagggttc gtccgcccat cgcttccagt tgctgccagg
     7681 cagtaaccac ctgctgtgcg gcctgttcag aaatcagccc gccaataaac ggctgcggtt
     7741 cgtcatccca gttgcccggc gttaatcgct ggctgacggc aaccagacga gcaagaaacg
     7801 catcgccctg cgctccgctt ttgagcaata aacggcgggc gcaggtgcag cgttgcccgg
     7861 ctgtgacaaa cgccgactga atggtcagat ggacagccgc gtcgatatcc gccacctcat
     7921 cgataattag cggattatta ccgcccatct caagggcgag aattttctcc ggctgaccgg
     7981 agagctggcg atgcagctgg tagccggtat tggcgctacc ggtaaacagc aaaccgtcga
     8041 gatcctccag cgcactcagc gcctgacccg tttcacgccc gccctgcacc aggttcagta
     8101 cgcccggcgg caagccagcc tgctgccata aacgcattac cgcttcgcca ctccacggtg
     8161 tcagttcgct gggtttaaag ataatggtgt tacctgccag caatgccgga acgatatgtc
     8221 cgttcggcaa atgaccaggg aaattatacg gcccaaacac cgccagcacg ccgtgcgggc
     8281 gatgtcgcag gctcgccgca ccgtccggca tttcactacg ctgctcgccg gtacgaacgt
     8341 gatacgcctt aattgatatc gcgattttat tgatcatcgc cgtcacttcg gttgccgctt
     8401 cccagcgcgg cttacccgtt tctctggcaa taatcgcggt taattcggct ttattgcttt
     8461 ccagcaatcc ggcaaagcgt tcgacaacga cctgacgttc agccaatgag agccgcgccc
     8521 agcgcggaaa cgccgctcgg gctgcccgac aagcctgttc gacctgagcg gcaccggcat
     8581 cattgccttg ccataacacc tcgcccgata ccggattacg cttcacacgc gatgcgccct
     8641 ggcccgttat ccagtcaccg ttaatccata aagtcatgct gttttctcct ctgcgcacag
     8701 gcgcaccaga cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt gtgcggcggt
     8761 taaaatcaaa cgctcggttg ccggatcggt acgcaccagc accacgcgga aatggtgata
     8821 attttcattg gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc cttctgccac
     8881 ttccaccagc cgacttttac ggatggcgcg cacgcggtcg atgtcacact caagcgttgg
     8941 cccgccgtcg aagatgtcga tatagttgcg gtaacgaaaa ccttctttct ccagcaccgc
     9001 gcgggcaggc gcggtttgcg gatgtacctg accgatgacg tcctgggctt cctgggataa
     9061 aaagtgggta tagatcggat gtttcggcat cagttctgca ataaatgcct tttgcccggt
     9121 gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac cgaggctttg
     9181 ccagaacggt gaatagccgt gttcgtcaat caccccgcgc atttcagcaa ccactttgtc
     9241 attaaacttg tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca gcaaatagcc
     9301 gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt
     9361 gtgatcgtta ctgagaaaca gcgtcggcaa tgcgttatag acattcagct cttttgaggc
     9421 gtgaaccaac gtgccgacgc gatagttgta ccagggatcg ttcagcccaa ccgccacctc
     9481 aatggcacaa atccccgcca cggtgcctgt ctcgctatct tccagcacga acacatagcc
     9541 ctgctcactt ttgggcagtt cgccttgcca ggttttgatt gccctttcga tacgcgccga
     9601 aagcgtggct tcattgg
//
LOCUS       DACAPB010000171         9550 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.171, whole genome shotgun sequence.
ACCESSION   DACAPB010000171 DACAPB010000000
VERSION     DACAPB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.171"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..380)
                     /locus_tag="IT904_003768"
     CDS             complement(<1..380)
                     /locus_tag="IT904_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAP3193487.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGT"
     gene            556..1929
                     /gene="mpl"
                     /locus_tag="IT904_003769"
     CDS             556..1929
                     /gene="mpl"
                     /locus_tag="IT904_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAP3193488.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESNFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2080..2631)
                     /locus_tag="IT904_003770"
     CDS             complement(2080..2631)
                     /locus_tag="IT904_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAP3193489.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            2725..4077
                     /gene="pmbA"
                     /locus_tag="IT904_003771"
     CDS             2725..4077
                     /gene="pmbA"
                     /locus_tag="IT904_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAP3193490.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4132..4518
                     /gene="cybC"
                     /locus_tag="IT904_003772"
     CDS             4132..4518
                     /gene="cybC"
                     /locus_tag="IT904_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAP3193491.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(4563..5027)
                     /gene="nrdG"
                     /locus_tag="IT904_003773"
     CDS             complement(4563..5027)
                     /gene="nrdG"
                     /locus_tag="IT904_003773"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAP3193492.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5186..7324)
                     /gene="nrdD"
                     /locus_tag="IT904_003774"
     CDS             complement(5186..7324)
                     /gene="nrdD"
                     /locus_tag="IT904_003774"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP3193493.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDKWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(7718..9373)
                     /gene="treC"
                     /locus_tag="IT904_003775"
     CDS             complement(7718..9373)
                     /gene="treC"
                     /locus_tag="IT904_003775"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP3193494.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(9423..>9550)
                     /locus_tag="IT904_003776"
     CDS             complement(9423..>9550)
                     /locus_tag="IT904_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIBC"
                     /protein_id="HAP3193495.1"
                     /translation="SIQPSYWQVFALAMAIAIIIPIVLTSFIYQRKYRLGTLDIV"
BASE COUNT         2228 a         2463 c         2550 g         2309 t
ORIGIN      
        1 atggtaccga cagagacgtt aacatcgatg ttggacgagc catccagggg gtccatcagc
       61 accacgtatt ttgcgtgttc acagccttca aagacgacaa tctcatcttc ttcttcagag
      121 gcaatccccg caacgatatc gcgtgctttc agtgcggctt tcagtttttc attagcgaac
      181 aagtcgagtt tttgctgaac ctcgccctgc acgttctcag caccgctggc acccaggata
      241 tcaaccagtc ctgctttgtt gatatcgcga tggataatct tggcgcccag ttttattgcc
      301 gacagcaaag cagtgagctc accggtagca tgagaaaact cgtgctgctt ttcgacaata
      361 aattcaccta acgttttcat aaaactttcc ctgcaatgtt tatggagtaa agcgaccgta
      421 acaatcttaa caaataatcg gaatgttgcg ctcaggtgaa tcgcgccagc aaattgcgga
      481 ttatccggaa atgcgtttct cacttgcccg acatatgcgt aaaatgagcg gcagattaaa
      541 aaaggatagt gacgtatgcg cattcatatt ttaggaattt gtggcacgtt tatgggcggt
      601 ctggcgatgc tggcgcgcca gttaggccat gaagtaacgg gttcggacgc caatgtgtat
      661 ccgccgatga gcaccttact tgagaagcaa ggcattgaac tgattcaggg ttacgatgcc
      721 agccagctcg atccgcagcc ggatctggtg attattggca acgccatgac ccgtggaaat
      781 ccgtgtgtgg aagcggtact ggaaaaaaac atcccttata tgtcaggtcc acagtggctg
      841 cacgattttg tgctgcgcga ccgctgggtg ctggccgttg ccggtacaca tggcaaaacc
      901 accaccgcgg gaatggcgac ctggattctg gaacagtgcg gttacaaacc gggatttgtg
      961 atcggcggtg tgccggggaa ctttgaggtt tcggcgcgtc tgggcgaaag caacttcttt
     1021 gttatcgaag cggatgagta tgactgcgcc ttcttcgaca aacgctctaa atttgttcat
     1081 tactgcccgc gtacgctgat cctcaacaac cttgagttcg atcacgccga tatctttgac
     1141 gacctgaaag cgatccagaa acagttccac catctggtgc gtatcgttcc agggcagggc
     1201 cgtattatct ggccagaaaa cgacatcaac ctgaaacaga ccatggcgat gggctgctgg
     1261 agcgagcagg agctggtggg cgagcagggt cactggcagg cgaaaaagct gaccaccgat
     1321 gcttccgaat gggaagtttt gctggatggc gaaaaagtgg gcgaagtgaa atggtcgctg
     1381 gtaggcgaac ataatatgca caatggcctg atggcgattg cagcggctcg ccatgttggt
     1441 gtagcgccgg cagatgccgc taacgcgctg ggttcgttta ttaatgctcg tcgccgtctg
     1501 gagttgcgtg gtgaagcgaa tggcgtcacg gtatatgacg attttgccca tcacccgacg
     1561 gcgattctgg caacgctggc ggcgctgcgt ggcaaagttg gtggtacggc gcgcattatt
     1621 gctgtgctgg aaccgcgctc gaataccatg aaaatgggga tctgcaaaga cgatctggca
     1681 ccttcattag gtcgtgccga tgaagtcttc ctgctgcaac cggcgcatat tccgtggcag
     1741 gtggcagaag tggcagaagc ctgcgttcag cctgcacact ggagtggcga tgtggatacg
     1801 ctggcagata tggtggtgaa aaccgctcag cctggcgacc atattctggt gatgagcaac
     1861 ggcggttttg gtgggatcca tcagaaactg ctggatggtc tggcgaagaa ggcggaagct
     1921 gcgcagtaat tcggcctcag cctgagatag cattgccgga taaggcgttc acgccgcatc
     1981 cggcatttga gcatagtgcc tgatgcgacg cttgatgcgt cttatcaggc ctacaggtgc
     2041 accgcatccg gcaaaccata ctcacatcaa caacgaaaat tacccttcat tctccgccag
     2101 ttcgcgcaga tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc
     2161 tttcttcgcg ttacggatca gagtacgcag ttgctgacga tcggcatcgg gccacagatt
     2221 taatacctca gcgatggcgt catcaccttg atcgatcagg cgatcgcgca ggttttccag
     2281 tttatggaac agcaccacct gctggttgtg acggtttttc agcttatcca gcgcctgacg
     2341 gatcggttct acatcgcgct ggcgcagcat cttaccgatg agctgcaact ggcggcggcg
     2401 accttccatc ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg
     2461 gatcttatcc agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc
     2521 ctcggcatca cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc
     2581 gatgtcatca ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc
     2641 cttaaaaaaa gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta
     2701 gacttcagag aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa
     2761 gcgcagcgca agattctgga agaagcagtt tccactgcgc tggagttggc ctcaggcaaa
     2821 tcggacggtg cggaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat
     2881 ggtgaagtgg agaatgttga attcaatagc gatggcgcgc tggggatcac tgtttatcac
     2941 cagaaccgca aaggtagcgc atcatccacc gatttaagcc cgcaggccat tgcccgtact
     3001 gtacaggcgg cgctggatat tgcccgttat acctcgccag atccctgtgc cggcgtggca
     3061 gacaaagagc tgctggcctt tgacgcacca gatctcgact tgttccaccc tgcggaagtt
     3121 tccccggatg aagccattga actggcggcc cgcgcagaac aggcggcatt gcaggcggac
     3181 aaacgcatca ccaataccga aggtggcagc tttaacagcc actacggtgt caaagttttt
     3241 ggcaacagcc acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt
     3301 gtaattgccg aagaaaatgg cgatatggag cgtgattacg cctacaccat tggtcgtgcg
     3361 atgagcgatc tgcaaacgcc agagtgggtt ggggccgact gtgctcgccg tactttatcg
     3421 cgcctgtcac cgcgtaaact ctccaccatg aaagcgccgg tcatttttgc caatgaagtg
     3481 gcaaccgggc tttttggcca tctggtgggg gcgatagcgg gtggctcggt ttaccgtaaa
     3541 tctacctttc tgctggattc gctgggtaaa caaattctgc cggactggct gaccattgaa
     3601 gagcatccgc atctgctgaa agggctggcg tcgacgccat tcgacagcga aggtgtgcgc
     3661 accgagcgtc gcgatattat taaagatggc atcctgactc agtggctgct gaccagctat
     3721 tcggcgcgaa aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg
     3781 attgccggac aagggctaag cttcgaacaa atgctcaaag agatgggcac cgggctggtg
     3841 gtgacggagc tgatgggcca gggcgtgagt gcaattaccg gtgattattc ccgtggtgcg
     3901 gcgggcttct gggtagaaaa cggcgaaatt cagtatccgg ttagcgaaat caccatcgca
     3961 ggtaatttaa aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc
     4021 agtaatatac agtgtggttc tgtgctgttg ccggagatga aaatcgccgg gcagtaaaaa
     4081 actggcgcga cctgccgcgc caaatcttca taataaaaaa ggaagtgagc aatgcgtaaa
     4141 agcctgttag ctattcttgc agtctcctcg ttggtattca gttcggcgtc gtttgccgct
     4201 gatcttgaag acaatatgga aaccctcaac gacaatttaa aagtggtcga aaaagccgat
     4261 aacgcggcgc aagtcaaaga cgcgttaacg aagatgcgcg ccgcagcgct ggatgcgcaa
     4321 aaagcaacgc cgccgaagct ggaaggaaaa tcgccagaca gcccggaaat gaaagacttc
     4381 cgccacggtt tcgacattct ggttggccag attgacgacg cgctgaagct ggctaatgaa
     4441 ggtaaagtaa aagaagcgca ggctgccgca gagcaactga aaacgacccg caacgcctat
     4501 caccagaagt atcgttaatt cctcatttcc ctgttgcctg cactcaggta acagggaact
     4561 tatcatcgca aatgatgcac cacctgattg ctgctgccgc gccagatcag ggaagggtct
     4621 tttaaatcct gcacaaattt gccgtcgacc agcacgttaa tcaaatcaac aacctgcatt
     4681 tgcgcagcgt tgagttcgtc gagtttatag cctgtccaca cccagatgtc tttacccgga
     4741 cactcggcgc ggatgcgttg taccagtttc agaatatccg gcacgttttg cggatgcagc
     4801 ggatcgccgc cggagaggga aataccctgg cgtttgatgc gagtgtcatt cagatcgtta
     4861 atgatctggt cttccattgc tttggtaaat ggctgaccgg aatttacccg ccaggtgctt
     4921 ttgttatagc aaccggggca ttcatgaaca cacccggaga caaacagggt gcagcgagtg
     4981 ccggggccgt tgacgatgtc gacaggatag tactgatgat aattcattgt ttcgcctggc
     5041 ttatcagtcc gcgtatagga tgcatccggt aatcaacgcc tgatgcgacg ctgtcgcgtc
     5101 ttatcaggcc tacaatcgtt gttgccatgt aggccggata aggcgtttac gccgcatccg
     5161 gcatttgctt aacgtagagt gaagattaac ctatctgccc attccccaaa tgtttaacgc
     5221 ggcgcttaac ttcttcctgc ttaccggcgt taaatggacg tgcatccggg ctacctaaat
     5281 atccgcacac gcggcgagtc accgacacac gggaggcgtc atggttacca catttcgggc
     5341 aagtgaagcc tttgctggtg cactcgaact caccggtaaa gccacactcg tagcactcat
     5401 caatcggtgt attggtgccg taatacggta catgctgata gctgtaatcc cagacatctt
     5461 ccagcgcctt caggttgtgc tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt
     5521 tcgccagcgg cgggtaaggc gcttcaaagt cgatcttgtc gtacgggttc accttcttct
     5581 ccacatcgag gtggaaactg ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc
     5641 caaactcagc agtatcgaga cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt
     5701 agagactgaa accataaccc gtttcttctt tccacttatc cactgcctga cgcagacgtt
     5761 caacaatggc gatacctttc gcgcgaagct gctcgttgtc gtaaacatgc tcgccgccga
     5821 acagcgcgtt aatggtttcg tggatgccga tgtaacccag cgaaatagac gcacgaccgt
     5881 ttttgaagat ttcagaaaca tcatcgtcag cattgagacg cacgccacaa gcaccttcca
     5941 tatagaggat cggggccacg cgcgctttca cgccttcgag acgagcgata cgggtcatca
     6001 gcgccttacg tgccagcacc agacgttcat ccagcagctt ccagaaggtg gcttcatcgc
     6061 cttttgcttc cagagcaata cgcggcaggt tcaggctgat cacgccgagg ttgttacgac
     6121 catcgtggat ctgctcgccg ttttcatttt cccacacgcc gaggaagctg cggcagccca
     6181 tcggggtttt aaacgaaccg gtgactttca ctacctgatc gtagttcagg atatccggat
     6241 acatgcgctt gcttgcgcac tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt
     6301 ttttatggtt cagaccatcg cgaatcgcaa acaccagttt cgggaacacc gcagttttac
     6361 ggtttttacc gaggcctgcg atacggttac gcaggataga ttcctgaatc aggcgcgatt
     6421 cccagctggt gcccagacca aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt
     6481 gcagggtgtt tacttcgtac tccagcgact ggaaggcatc gtagcactct ttaatggtac
     6541 gagagttagc gtagccttcg gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt
     6601 tgttgtagct ggcggtgaca aacggtgcca gcacttcatc gatacggtta atggtggtgc
     6661 cgccataaat atggctggcg acctgagcaa taatctgcgc agttacagcg gttgccgtag
     6721 agatcgactt cggcggttca atctcggcgt tccccatttt aaagccctgg gtcagcatgc
     6781 ctttcaggtc aatcaacatg cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt
     6841 gatagtgaat atcgccacgc tcatgtgcct gcaccacgtc acgcggcagc aggtgctggc
     6901 gtgcatagtg tttagccacg atcccggcca gcaggtcgcg ctgggttgga atcaccttgc
     6961 tgtctttgtt ggcgttttcg ttgagtaacg aggcgttggt ctgctcgacc agaccacgga
     7021 tctcctggtt caggcgaccg cgtttttcac gttcaatgtc gcgatcgtga cggtactcga
     7081 tgtaagcacg agccagttgt ttgtatggac ccgacatcag ctggttttca actgcggtct
     7141 ggatctcgtt gatatccacc tggttgcggc cctgcatctg ctcgctgaca accgcagcaa
     7201 cagtggcgca ataatcggca tcatcgactt ccgctgcttt agctgcacgc agaatcgctt
     7261 ctttgatgcg ctctgattta aacggcactt tgcagccgtc tcgtttcatc acatgcggtg
     7321 tcatgatcgc tccatatttt taagaacagg ttatccacag aaattgggaa aggcgttttc
     7381 cggttttttc gtttcgtttt ccggtgcttt ccgcaatcgc catccgctta tccacaacgc
     7441 cgcacctttt tcgtggggag ctgtagtagc aattatagtc gattaataca acatattggg
     7501 ttgggacgca ttttaaagtc tatatatagt gctttgcatc aaggatgttt gaactttttt
     7561 tgatgtagct caaagtaaaa agcagagcgt acggatgacg ggcgctacag cgttatgtaa
     7621 attttttaat gaatttgctg gttgaaaaat caacaaaaac aacatgctga cagacaaaac
     7681 cccggaatga ccggcattac cggggcttag ggaagattta cttctgtaac caccagacag
     7741 cctcaaaagg ccgtaaattc atggcacagg gttgtggtga ggcttcttcg tagttatgca
     7801 tcacaagctg ccagttgccg cgcatttgcc ctggctgcca gggttggatc ccacggctaa
     7861 ggttggcaat gaccagcaag gtttgcccct tccattcacg gcgatagcac cacaatacag
     7921 ggctgtttgg cagcagatcc tggtaattgc cccatgtcag aacggcttct tgcttacgca
     7981 gtgcgattaa cttttggtag gtgtaaaaca ccgaggactc atcggtcagc gcggcttcta
     8041 cgttgatttg ttgatagtta tcgcccaggc caatccacgg ttcgccagcc gtaaacccgg
     8101 cattatcgcc gttgctccat tgcatgggcg tgcgactgtt gtcacgggat ttactggcga
     8161 ggattgccaa taactcgtcg gcatcacgcc catcgttgcg cagctcggca aacatattga
     8221 ggctctccac gtcgcgatag tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct
     8281 cttcgccctg gtagatatac ggcgttccct gcatgccatg cagcaccatc gccagcattt
     8341 ttgccgcagg cacgcggtat tcaccttcat caccaaagcg agaaacaatg cgcggctgat
     8401 cgtggttaca ccagaacaag gcattccatg ccacgttgtg cattccttgt tgccagtggc
     8461 ggaacaatgt tttcaacgcc acaaagtcag gtttagccag cgtccatttt tcaccaccgg
     8521 gataatcgac cttcaggtga tgaaaattaa aggtcatcga caattcactg cctgtcagtg
     8581 ccgcgtatcg ctggcaatgc tcaaggctgg tggaggacat ttcacctacg gtcattaacc
     8641 cgcgtggcgt aaacacatcg cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt
     8701 cggtgtagaa gcgacgcccg tcgccatcca ggtcttcagg gaaacgcggg tctttggaga
     8761 tcagattcac cacatccagg cgcaacccgt cgaccccacg atcggcccag aattcacaga
     8821 cttttttcag ctctgcgcgt accgctggat tctcccagtt gagatccgcc tgttctggtg
     8881 caaagagatg caaatagtac tgttcgcttt ccgcatgcca gcgccacgca ctaccgccaa
     8941 attttgaacg ccagttgttc ggtggcgttt ctggttcgcc atcgcgccag atataaaact
     9001 ggcggtaagg gctttctttg ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat
     9061 ggttaaacac catatcgaga atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt
     9121 cgtcaaaatc gtccagcgtg ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt
     9181 aaccgttatc gacctgggga gagacataaa agggggttag ccagatggca tcaacgccca
     9241 gtttatgcag atagtccagg cgttggataa cgccacgtaa atcgccggtg ccgctacccg
     9301 tggtgtcctg aaaactcttt ggataaatct ggtagataac gccgttttgc caccagtggg
     9361 gaagattagt cattacgtta ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa
     9421 aattaaacaa tgtccagcgt gcccaggcgg tatttccgct gatagataaa cgaggtgagt
     9481 acaatcggga tgatgatggc gatagccatt gccagcgcaa atacctgcca gtagctcggt
     9541 tgaatcgaga
//
LOCUS       DACAPB010000172         9534 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.172, whole genome shotgun sequence.
ACCESSION   DACAPB010000172 DACAPB010000000
VERSION     DACAPB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.172"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            325..654
                     /locus_tag="IT904_003777"
     CDS             325..654
                     /locus_tag="IT904_003777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193496.1"
                     /translation="MSLGVIAGNTLIIKEKYKQDTVMNDTCDTMTPISIKYNDHCYII
                     TVVVIFFKNAVIGVIGVISNVATLAGYGLRTMTPTMTPYKLMTPRRHFENKKSPGIRQ
                     GNGQQLL"
     gene            complement(657..881)
                     /locus_tag="IT904_003778"
     CDS             complement(657..881)
                     /locus_tag="IT904_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193497.1"
                     /translation="MSYRAYIIAFDPEHGTYTETEIMEGFATEQEAVDHARNRLPEVQ
                     QELAKLGENLLCSYRIRVVDSAEILPFIRS"
     gene            complement(891..2471)
                     /locus_tag="IT904_003779"
     CDS             complement(891..2471)
                     /locus_tag="IT904_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HAP3193498.1"
                     /translation="MGKQVAGDTTPPVYLGPRQLDDLDNLRIIDDGRRSARVYLAGDI
                     KQLQISTIADKLAVAGVKEARLFKGITDREPEKWNMDRLRDAALRGESLVGMLRKEER
                     ATAAATVKDIHDMAPVLMTTWPNIGSNGQPLNTRPNVERLLDNYSISAKYNEISKDVE
                     VLVPGVSGGSDARDNCAVSEILSLAALNRLPMSNIEGHIKTIAVRNTYNPVRDFINQR
                     EWDGRSRFADLLNTISTPDDYSRDLLAMLVRRWLLSAVAAAYLEAGFWSKGVLVFQGE
                     QSLGKTAWFKALLPHDNRNLVKVGATIDPGNKDSVASAISYWLVELGELDATFRKADI
                     AKLKAFISQDRDDLRRPYDRLESKYQRRTVFFASVNPKHFLADDTGNVRWWTIPVTAV
                     NYEHGIDTQQLWAEVLSWFEAGERWWLDRDEEAMLEVVNEQHGQTDPIEEMILARFDW
                     NSDRLAAYIEMTATDVLLSIGQDRPTKSQATQCGNILRKLTGRDARRTSKGRFYLMPP
                     KVFGQQDHRYFDGPNHQF"
     gene            complement(2563..2844)
                     /locus_tag="IT904_003780"
     CDS             complement(2563..2844)
                     /locus_tag="IT904_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193499.1"
                     /translation="MKKTTVNQAISRYNSLLRNPQRQLTVGELTAQRMAAAQLLLQAC
                     IREGVNRPWTIVSRHAAMADSLVPFRISDSESWAMYLELKRGVRYEKRA"
     gene            complement(2853..3032)
                     /locus_tag="IT904_003781"
     CDS             complement(2853..3032)
                     /locus_tag="IT904_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP3193500.1"
                     /translation="MELLTAKQVTTLTTLSRMTIWRYIQAGTFPNCIKLGPKRVAWRR
                     KDVMAWLESRQPVQR"
     gene            complement(3327..4562)
                     /locus_tag="IT904_003782"
     CDS             complement(3327..4562)
                     /locus_tag="IT904_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3193501.1"
                     /translation="MAENKLSDVQLRALTRKVIEKPFDVADGGSLSVRVTPSRRKILD
                     GEKRPANNIQWLFRYKNKHKSANTLTLVLGKYPALSLAEARVKRDQCKRWLAHNLDPK
                     DQFDQESAKTMKPVTIREALERWINEYAAENRVNFERHRRQFERHIYPFIGELPLEQC
                     SKARWMETFSRIKKVAPVASGYILGNCKQALIHNRRLHDVYSDALEDIKVTDVGRKQA
                     KRDRVLTSEELSGLWNALKSEYAFLPYYTALLKLLIVFGARTQEIRLSTWSEWNVKEW
                     IWTVPREHSKGGEKIIRPVPESIQPFILQLRQNHLDSGMLLGEMKKPEAVSQWGRILY
                     RRLEHSTPWTLHDLRRTFATSLNNLGIAPHVVEQLLGHSMPGVMAVYNRSQYIPEKRL
                     ALDKWIKYLEKINLLKSDN"
     gene            4832..5716
                     /gene="yhdJ"
                     /locus_tag="IT904_003783"
     CDS             4832..5716
                     /gene="yhdJ"
                     /locus_tag="IT904_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP3193502.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            5800..5979
                     /locus_tag="IT904_003784"
     CDS             5800..5979
                     /locus_tag="IT904_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAP3193503.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(5982..6644)
                     /gene="acrS"
                     /locus_tag="IT904_003785"
     CDS             complement(5982..6644)
                     /gene="acrS"
                     /locus_tag="IT904_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAP3193504.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            7043..8197
                     /gene="acrE"
                     /locus_tag="IT904_003786"
                     /pseudo
     CDS             7043..8197
                     /gene="acrE"
                     /locus_tag="IT904_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
     gene            8212..>9534
                     /gene="acrF"
                     /locus_tag="IT904_003787"
     CDS             8212..>9534
                     /gene="acrF"
                     /locus_tag="IT904_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HAP3193505.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGA"
BASE COUNT         2490 a         2288 c         2187 g         2569 t
ORIGIN      
        1 aacttataac cataaatact tgcaaataat ccggcataat ccggcgcaat cgatatttac
       61 acatctttac aattcagcgt gtgtaaaacg tgttcgcttt attttgcaat cgcgcacgta
      121 tggtgccagt gctttgatag gattcacata aatgattgat ttacttgacg taatcataaa
      181 gtgagttttg taggggcacg cacaacgcga attacgcggg accaatgccc ccgcaggctg
      241 tacccccctc ggaagtacct tgactttttt ggcagcagat ggcgatgaca cctaatgtca
      301 ccatgacggc gttatgttgg aaaggtgtca ttaggtgtta tagcgggaaa cacgctgatt
      361 attaaagaaa aatacaaaca agatactgta atgaatgaca cctgtgacac catgacaccc
      421 atttctataa agtataacga ccactgttac attattacag tggtcgttat tttctttaaa
      481 aatgctgtca taggtgtcat aggtgtcatt agtaatgtcg caacccttgc gggttatggc
      541 ttgagaacaa tgacacctac catgacacct tataaattaa tgacacctag gcgtcacttt
      601 gaaaataaaa aaagccccgg cattcgccag ggtaatgggc aacaactgtt gtgatgttat
      661 gatcggatga acggcaagat ttcagcggaa tccactaccc ttatgcggta gctgcatagc
      721 aggttttcac ccaacttggc cagttcctgc tgcacctctg gcagcctatt gcgagcatgg
      781 tctaccgctt cctgttcagt ggcgaaaccc tccataattt cggtttcggt gtaggtgccg
      841 tgttccgggt caaaagcaat gatatatgcg cgataactca tagtattacc tcaaaactgg
      901 tggttaggcc catcaaaata gcgatggtct tgctgtccaa aaactttcgg tggcattagg
      961 taaaaacgcc ctttacttgt tctacgtgca tcccttcctg tcagttttcg cagaatgtta
     1021 ccgcactgcg ttgcctggga ttttgttggc cgatcctgcc caatagacaa cagaacatcc
     1081 gttgcagtca tttctatata tgcagccagg cgatcactgt tccaatcaaa acgcgccaat
     1141 atcatttctt caatcgggtc agtttgcccg tgctgttcgt tcacgacttc cagcatggct
     1201 tcttcatcac ggtccagcca ccagcgttca cctgcttcaa accaggaaag aacttcggcc
     1261 cataattgtt gtgtgtctat gccatgctcg tagttaacgg cagttacagg aatggtccac
     1321 cagcgaacgt tcccggtgtc atcggccagg aagtgtttcg ggttcactga cgcaaaaaac
     1381 acggtgcggc gctggtactt cgattcaagc cggtcataag ggcggcgcag gtcatcacgg
     1441 tcctggctga taaatgcttt gagcttggca atatccgcct tgcgaaacgt cgcatccaat
     1501 tcgcctagct caacaagcca gtaactaatt gcgctggcca cgctgtcttt attgccgggg
     1561 tcaatagttg ctcccacctt aaccaggttg cgattgtcat ggggcagaag cgctttaaac
     1621 catgctgttt tacccagtga ctgctcgccc tggaaaacca gcacgccttt ggaccagaaa
     1681 cccgcttcta agtaagcagc cgccacggca gacagcaacc aacgacgcac cagcatggcc
     1741 agcagatcgc gactgtagtc atctggcgtg ctgatagtat tcagcaggtc tgcaaatcgg
     1801 cttcgtccat cccattcacg ttgattgatg aaatccctga caggattata agtattgcgc
     1861 acagcgatag ttttgatatg accttcaata ttactcattg gtaaccggtt cagtgcggcg
     1921 aggcttaaaa tttcactcac agcgcagtta tcgcgagcat ctgaaccgcc actaacgcca
     1981 ggtactaaaa cctcaacgtc cttgctaatc tcattatatt ttgcagaaat gctgtagtta
     2041 tctaatagac gttcaacatt agggcgtgtg tttaaaggct ggccattgct tccaatattt
     2101 ggccatgttg tcattaatac aggagccata tcgtgaatgt cttttacagt tgctgcggcg
     2161 gttgctcgtt cttctttgcg cagcatgccc accaggcttt caccgcgtag ggcggcgtct
     2221 ctaagcctgt ccatgttcca tttttccggt tcacggtcag taatgccttt aaataaacgg
     2281 gcttccttaa ctccagccac tgccagcttg tcagcaatag tgctgatttg aagttgctta
     2341 atatcaccag caagatatac ccgcgcagac cggcgaccat cgtcgataat tcgtagatta
     2401 tctaaatcat ccagttgtct tggcccaaga tagacaggag gagtagtgtc accggcaact
     2461 tgtttaccca ttccttcttc ccagcccttg gcatggctcc aagcgtcaga accggcaaaa
     2521 ataattgctt ccgtaaaagg gtctttaggt aatagcttaa cgttaggcgc gtttctcata
     2581 tcgcaccccg cgttttaact ccagatacat cgcccatgat tcagaatcgc tgatgcgaaa
     2641 cggcaccagg ctatcagcca tagcggcgtg acgtgaaaca atagtccacg ggcggtttac
     2701 accttcgcga atgcatgctt gcaacaataa ttgtgctgcc gccatacgct gggcggtaag
     2761 ttcgccaacg gttaactggc gctgtgggtt acgcaacagg ctgttatagc gactgatagc
     2821 ctgatttacg gttgttttct tcataagcca cctcagcgct gtactggctg gcggctttca
     2881 agccacgcca taacatcttt gcgccgccag gccacgcgtt ttggccctaa ttttatgcaa
     2941 ttaggaaacg tgccagcctg gatatagcgc cagatagtca tgcgggataa tgttgttagt
     3001 gttgtgactt gcttagcagt cagtaattcc atcttacggc cctcaaaaca tggccggtgg
     3061 atatgtggtc tgtaatgatt tatgattatg ttgcgttaac aaagaacgcg accattacag
     3121 ccttcaccgg ctttttggta ttaaggggtt cggttttctg tttggcgacg tgaaccgatc
     3181 cccgccttat tcaagccctc agttctgctg ggggcttttt attactacat gacaataata
     3241 ctcaaattgt atcagcagta aatacccttt aaattcataa acttaactgc aaataacgct
     3301 acataactct atataatcat caatttttaa ttatctgatt ttaaaaggtt tattttttct
     3361 aaatatttta tccatttgtc caaggctaac cgtttttccg ggatgtactg gctacgattg
     3421 tacaccgcca tcactcctgg cattgagtgg cccagcaact gttcaaccac atgcggggct
     3481 atccccaagt tatttagact ggtggcaaaa gtgcggcgca ggtcatgcaa cgtccacggc
     3541 gttgaatgct ctaatcgtct gtataagata cgcccccact ggctgacagc ttcgggtttc
     3601 ttcatttcac caagcagcat tccgctatca agatgatttt ggcgtagctg taaaataaat
     3661 ggctgtatag attcaggcac gggtctgatg attttctccc cacccttgct atgttctcgc
     3721 ggaactgtcc aaatccattc tttcacgttc cattccgacc atgtcgaaag acgaatttct
     3781 tgtgttcttg caccaaacac tattagcaat tttaaaagcg cggtgtagta aggtagaaac
     3841 gcatattcgc tttttagcgc attccaaagg ccactaagtt cttcagaagt taaaacacga
     3901 tcacgttttg cctgctttct tccaacatca gtaactttga tatcctccag cgcatcactg
     3961 taaacatcgt gcagcctgcg gttgtgaatc aacgcctgct tgcagttacc taatatgtag
     4021 ccgcttgcta ccggcgcgac ttttttgatt ctgctaaatg tctccatcca tcttgcttta
     4081 gagcactgtt caagtggcaa ttcgccgatg aaaggataaa tatgtctttc gaattggcgt
     4141 ctatgccttt caaagttgac ccggttttcc gccgcgtact cattaatcca gcgttccaat
     4201 gcttccctga tagtgacagg tttcatggtt tttgctgact cttggtcaaa ctggtcttta
     4261 ggatctagat tatgagcaag ccaccgtttg cattggtcgc gtttcaccct cgcctcagca
     4321 agagataagg caggatactt gcccagcact agcgtcaaag tattggcgct tttatgctta
     4381 ttcttataac ggaacaacca ttggatgtta ttggccggtc tcttctcgcc atcaaggatt
     4441 ttgcgccttg atggtgtaac gcgaacagaa aggctgccac cgtcagcaac atcaaaaggt
     4501 ttttcgatga ctttgcgggt taacgcacgc aattgaacat ctgagagttt attttcggcc
     4561 atagagttaa tgacaacctt tttgacaact gtatgcaggg agtaactgat aactgatgat
     4621 acaatgcgag ccaataaaaa ataaatatct ctttccgcat caattattta tgatgaatac
     4681 aaattatcta gagtgataat aaattacgta taatgactct gtattttaca atacggcatg
     4741 aactaattca ggttagctaa atgcttgatt aaaaaggcgc tactcggcat ggggaagcgc
     4801 cttttttata ggtgtcacaa agggagtgac catgagaaca ggatgtgaac cgacccggtt
     4861 tggtaatgaa gctaagacca ttattcacgg tgatgccctt gccgaactta aaaagctacc
     4921 tactgaaagc gtcgatctga tctttgccga cccaccgtat aacatcggta aaaattttga
     4981 tggtctgatc gaagcctgga aagaagatct gtttatcgac tggctgtttg aagtgattgc
     5041 agagtgccac cgcgttctga aaaagcaggg cagcatgtac attatgaaca gtacggaaaa
     5101 catgcccttt atcgatctcc agtgccgcaa gctttttacc atcaaaagtc gcatcgtctg
     5161 gtcatatgac agttctggag tacaggcgaa aaaacactac ggctccatgt acgaacccat
     5221 cctgatgatg gtgaaagacg caaagaacta cacattcaac ggtgatgcta ttctggtaga
     5281 agccaaaacc ggatcgcagc gcgcgttgat cgattatcgc aaaaatcctc cacagccata
     5341 caatcatcaa aaagtaccgg gtaacgtctg ggattttccg cgcgtgcgtt atttaatgga
     5401 tgaatatgag aaccacccga cgcaaaaacc ggaagccttg ctgaaacgca ttattctcgc
     5461 ctcttccaac ccaggcgata tcgttctcga cccgtttgcc ggtagcttta ctaccggtgc
     5521 cgtagccatc gccagcggac gaaaattcat tggtattgag atcaacagcg agtacatcaa
     5581 aatggggctt cgacggctgg atgtcgcgtc gcattactcc gcggaagaac tggcgaaagt
     5641 gaaaaaaaga aagacgggca accggtcaaa acgatgccgg gttagcgaag ttgaccccga
     5701 tctcattgca aagtaaagga agtgtaagcc tggttttcag attattcatt tcgtgtatat
     5761 ttcctgccag acttggttaa acatgcacag gctcaggtaa tgattcgcaa gtattggtgg
     5821 ctcgtcgttt tcgctgtctt cgtttttctg tttgatactt tactgatgca gtggattgaa
     5881 ctgctggcaa cagaaacaga caaatgccgt aatatgaact cagttaatcc actaaaactg
     5941 gttaactgtg acgaactgaa ttttcaggac agaatgtgaa tttacatgac acttaattca
     6001 ttcgtttgat gaattaattt cgttatgttt tcatctggca tgaacattct taatacgtta
     6061 tcgaccagcg cgggtgcttg tttataaaga tcataacccg ccatattcat taaccagttt
     6121 tgaacaattc cgctgaaggc accatcaata ataatcatca caacatctaa atcgaggtta
     6181 tttgctacac aaccttgttg ctgacacgcc tgcaatactt cgcggagagt ctgcggatta
     6241 aagcccatct tttcgcgtat cactccctcg gccagcatct catcattaaa ttcacatttg
     6301 tgatataaga ttttcagcaa cgcctgctgg cggggaattt tggcaatata ttgcaagccg
     6361 acaatcaatt tttcacgcaa ttgttgaaac gggtcatgct ctaatccagc cgtcaagtgt
     6421 tcctggatta actcccgcaa tgaaggctgt tgcaaccaca tctcattaaa cagttgggtc
     6481 ttgttctcga agtgccagta gatagcgcca cgcgtaacgt tagcggcatc ggcaatgtcg
     6541 ttgagcgtcg tcttgcttac gccatgttgc gcaaactggg cgatggcagt ttcaatcagt
     6601 tcttgccggg tcttcagagc ttcggctttg gttctttttg ccatgattaa ttattcagga
     6661 aataaatata ttcgacacag ggtgagaaaa tagcgaaggt taatctatca tctaatgtgt
     6721 atttatacga gagggtaaca ttgagttgct ataaatcgtc aaataaataa tatatattat
     6781 ttacctaaaa tacattcgcc acatgaatat atatttcagt ttacgaggtt ttaattctac
     6841 ctctttcaac ccgcgtcaaa ataaaacagt agaatattaa tctttttttg tgtttatgtg
     6901 ccttgagacg ccagtattca taactattcc ttacatcgac gaatgataat ttgtaggata
     6961 gcaaactgta tttttctttc tgcgagttaa cgcgttgcct ttctgggtaa ataacgcgct
     7021 tttggttttt tgaggaatag taatgacgaa acatgccagg tttttcctcc tgccctcctt
     7081 tattctgatc tccgcggctt taatcgccgg ttgtaacgat aagggagaag agaaagctca
     7141 cgtcggtgaa ccgcaggtta ccgttcatat tgtgaaaacg gccccgttag aagttaagac
     7201 tgaattacca ggccgcacca atgcttaccg tatagccgaa gttcgcccac aggttagcgg
     7261 gatcgtactg aatcgtaatt tcactgaagg cagcgatgta caagcaggcc agtccctgta
     7321 ccagatcgat cccgcgacct atcaggcaaa ttatgacagc gcgaaaggcg aactggcgaa
     7381 aagtgaagcc gccgccgcca tcgcgcattt gacggtaaaa cgttacgttc cgctcgtggg
     7441 tacgaaatac atcagccagc aggagtacga ccaggccatt gctgatgctc gccaggccga
     7501 tgccgccgtg attgccgcaa aagccacagt cgaaagcgct cgcatcaatc tcgcttatac
     7561 caaagtgact gcgccaatta gcggacgtat cggcaaatcg accgtgaccg aaggcgctct
     7621 tgtcactaat gggcaaacga ctgaactggc gactgtccag cagctcgatc ctatctacgt
     7681 tgatgtgacc caatccagca acgattttat gaggctgaag caatccgtag agcaaggaaa
     7741 tttgcataag gaaaacgcca ccagcaacgt agagttggtc atggaaaacg gtcaaaccta
     7801 tcccctgaaa ggtacgctgc aattctctga tgtgaccgtt gatgaaagca ccggctccat
     7861 aaccctacgt gctgtcttcc ctaacccgca acatacgctt ttgccgggta tgtttgtgcg
     7921 tgcacggatt gatgaaggcg tccaacctga cgccattctt atcccgcaac aaggcgttag
     7981 ccgcacaccg cgtggtgatg caaccgtgct gattgttaac gataaaagtc aggtggaagc
     8041 gcgccctgtc gttgccagtc aggcgattgg cgataaatgg ttgattagcg aaggtctgaa
     8101 atctggcgat caagtcattg tcagcggcct gcaaaaagcg cgtccgggag agcaggttaa
     8161 agccactacc gatacccccg cagatactgc atcgaagtac ggtaatctga catggcaaac
     8221 ttttttattc gacgaccgat atttgcatgg gtgctggcca ttattctgat gatggcgggc
     8281 gcactggcga tcctacaatt gcccgtcgct cagtatccaa ccattgcacc gcctgcggtt
     8341 tctgtttcgg caaactatcc aggtgctgat gcgcagaccg tgcaggatac ggtgacgcag
     8401 gttatcgaac agaatatgaa tggtatcgat aacctgatgt atatgtcctc caccagcgat
     8461 tccgccggta gcgtgacaat tacccttacc ttccagtccg ggaccgatcc tgatatcgcg
     8521 caagtgcagg tgcagaacaa actccagctc gccacgccgt tgctgccgca ggaagttcag
     8581 cagcagggga tcagtgttga aaagtccagt agcagctatt tgatggtggc gggctttgtc
     8641 tctgataacc cagacaccac acaggacgat atctcggact atgtggcttc taacgttaaa
     8701 gatacgctta gccgtctgaa tggcgtcggt gacgtacagc ttttcggcgc acagtatgcg
     8761 atgcgtatct ggctggacgc cgatctgcta aacaaatata aactgacacc ggttgatgtg
     8821 attaaccagt tgaaggtaca gaacgatcag atcgctgccg gacagttggg cggaacgcca
     8881 gcgttaccag ggcaacagtt gaatgcctcg attattgctc agacgcggtt aaaaaatccg
     8941 gaagaattcg gcaaagtgac cctgcgcgta aacagtgacg gctcggtggt gcgtctgaaa
     9001 gatgtcgcac gggttgaact tggcggtgaa aactataacg ttatcgctcg catcaacgga
     9061 aaaccggcgg cgggcctggg gattaagctg gcaaccggcg cgaatgctct cgataccgcg
     9121 aaagccatta aggcaaaact ggcggaatta cagccattct tcccgcaggg aatgaaggtt
     9181 ctctaccctt atgacaccac gccattcgtc cagctttcta ttcacgaagt ggtaaaaacg
     9241 ctgttcgaag ccattatgct ggtgttcctg gtgatgtatc tgttcttgca gaatatgcga
     9301 gcaacgctga tccccaccat tgcggtaccc gtggtgttgt tagggacgtt tgccatcctc
     9361 gccgcttttg gttactccat caacacacta acgatgttcg ggatggtgct tgccatcggg
     9421 ctgctcgtcg atgatgcgat agtggtggtg gagaacgtcg agcgcgtgat gatggaggat
     9481 aagctcccgc caaaagaagc gacggaaaaa tcaatgtcgc aaattcaggg cgca
//
LOCUS       DACAPB010000173         9289 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.173, whole genome shotgun sequence.
ACCESSION   DACAPB010000173 DACAPB010000000
VERSION     DACAPB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.173"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..259
                     /locus_tag="IT904_003788"
     CDS             <1..259
                     /locus_tag="IT904_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP3193506.1"
                     /translation="KRMIDHANAISLEVNISALAIAEAKGKIKNNEVDVVLLGPQVRF
                     QKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQQ"
     gene            374..1696
                     /locus_tag="IT904_003789"
     CDS             374..1696
                     /locus_tag="IT904_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP3193507.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTVIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLVIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1724..2035
                     /locus_tag="IT904_003790"
     CDS             1724..2035
                     /locus_tag="IT904_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP3193508.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            2091..3763
                     /locus_tag="IT904_003791"
                     /pseudo
     CDS             2091..3763
                     /locus_tag="IT904_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008460008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
     gene            3788..5227
                     /locus_tag="IT904_003792"
     CDS             3788..5227
                     /locus_tag="IT904_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP3193509.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWLWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5284..5502)
                     /gene="marB"
                     /locus_tag="IT904_003793"
     CDS             complement(5284..5502)
                     /gene="marB"
                     /locus_tag="IT904_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAP3193510.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQPTQPVVTSCGNVVVAPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5534..5917)
                     /gene="marA"
                     /locus_tag="IT904_003794"
     CDS             complement(5534..5917)
                     /gene="marA"
                     /locus_tag="IT904_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAP3193511.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5937..6371)
                     /gene="marR"
                     /locus_tag="IT904_003795"
     CDS             complement(5937..6371)
                     /gene="marR"
                     /locus_tag="IT904_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAP3193512.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6582..7247
                     /gene="marC"
                     /locus_tag="IT904_003796"
     CDS             6582..7247
                     /gene="marC"
                     /locus_tag="IT904_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAP3193513.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSAHIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(7272..8462)
                     /gene="ydeA"
                     /locus_tag="IT904_003797"
     CDS             complement(7272..8462)
                     /gene="ydeA"
                     /locus_tag="IT904_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAP3193514.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8612..8938)
                     /gene="yneK"
                     /locus_tag="IT904_003798"
                     /pseudo
     CDS             complement(8612..8938)
                     /gene="yneK"
                     /locus_tag="IT904_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(8969..>9289)
                     /locus_tag="IT904_003799"
     CDS             complement(8969..>9289)
                     /locus_tag="IT904_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP3193515.1"
                     /translation="RRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYD
                     NACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN
                     KNLA"
BASE COUNT         2548 a         2012 c         2195 g         2534 t
ORIGIN      
        1 taaacgtatg attgatcatg ctaacgctat ttcacttgaa gttaatattt ccgcattggc
       61 gattgcagag gctaaaggaa aaattaaaaa taacgaagtt gacgttgttt tacttggccc
      121 gcaagttcgc tttcagaaac cggagattga agccgttgca caggggaaaa tgcctgtagc
      181 cgttatcgag atgaaagact acggcaccat gaacggacag gcagttcttg aatttgcgat
      241 gaaactactg caacagtaag aatctaatat tattaaacta ttacctgaac taatttttca
      301 cacatcataa aatggatatg gcatacatgc tttattggga tgtataaata tccaaaacaa
      361 caggtataaa aaaatgggtt taatggcctc attcgaacgt ggaatggaac gttttcttgt
      421 tccagttgct atcaagttaa actcacaaaa acatgttgca gcggtgagag atggattcgt
      481 ttttacgttt ccaattatca tggcaagttc attaattata ttaattaact ttgccatatt
      541 atcgccagac ggttttattg ccggattact gcatctgaat agcattttcc ccaaccttga
      601 aaaagcacaa gctattttta ctccggtaat gaatggttct gtaaatatca tgtcaattat
      661 gattgctttc ctggtcgcca ggaatatggc gattagctat gagcaagatg atcttttatg
      721 cggattaacg gtaataggag cattttttat tgtttatacg ccatatcaga tgatagatgg
      781 gcaagcattc ctgacgacca aatatctcgg cgcgcagggg ttgtttgttg ctgttatcgt
      841 tgcattgatc accagtgaaa tattttgtcg cttagctcga aacccaaaaa tcaccataac
      901 gatgccggca gctgtaccgc ctgcggtagc gcgttcattt aaagttttat tgccaatatt
      961 ttttgtcatg gtgttctttt ccgcacttaa ttattgcctg acactgatat ccccggcagg
     1021 actaaacgat cttatttaca cattaatcca gacgccgctc aaacatatgg gaacgaatat
     1081 ctttgcggta attatcctgg gggctgtggg taatttcctg tgggtgctgg tgatccacgg
     1141 acctaatacc acatcggcaa ttcgagaaac cgttttttct gaggctaatc tggagaatct
     1201 ctcctgggcc gctcaacatg gcactacctg gggcgcgcca tatccgatta cctggacttc
     1261 tattaatgat gcattcgcca actgcggcgg ttcaggtatg acgttggggt tattgttggc
     1321 tatttttatc gcttctaagc gtgcggaata ccgtgatctg gcaaaaatgt catttatccc
     1381 cggtattttc aatatcaatg aaccgataat gtttggcctt cctattgtac ttaaccccat
     1441 catgatggtg ccgtttatta tggttcccat tgttaactgt gccattggtt acttctttgt
     1501 ttcgatggaa attattccac cggttgctta tgccgtgccc tggactacgc ccggaccttt
     1561 aattgctttc ctcggaaccg gggggaactg gctggcttta ctggttggtt ttttatgttt
     1621 aggtgtggcg acaatgatct atttaccttt tgttattgcc gccaacaaag tcaataacat
     1681 gacaactaac ggataattcc atttacccag gaaggagatt gtgatgtttg cagatgaaga
     1741 acttgttatg gagttactgg tcaacgcggg gcaggcacgt tctgacgcga tggaagccat
     1801 acgttgtgcc gggcaaaaag actggcaggg cgcaactcag ctaatggcca gttctgaatc
     1861 tgcctgtctt caggcacata agattcagac cgcattaatt agtcaggatg aaggctgtgg
     1921 caaaataaaa gttaatttaa ttctggttca tgcacaggat catttgatga atgcaatatt
     1981 atgccaggat ttagccaggg aaattatttc attaagaaaa gaactccatg cctgattttt
     2041 tcctccagtg cgatagctgg aggaaattat tatactctta aggataagtc atgaatataa
     2101 agacgcttaa catcagcctt ttgtcttttt ctattattac agcattgttt ccatcgaacg
     2161 cgatggcaac aaaattaacc atagagcagc gccttgaatt gcttgaaaat gaattgtcgc
     2221 aaaataaaca agagctgaaa gcaacacaga atgaactggg agtatataaa tcccgacttt
     2281 cgaccttaca aaaaagcatc acggaaaaca aatataaatc agcctcgctt gctgaaatat
     2341 cagcgacatc tcccgtcgct gataacatca aaaatgaaaa cggtgaacag aactcgtctg
     2401 ccgcagcgca tactataaat ggatcgcagc aagttgccgt tattgaaagt aaaggcgaca
     2461 aaaccactat cgaaagcgtg accctgaaag atatcagtaa atatataaag gatgatattg
     2521 ggttcagcta tcaggggtac ttccgctcag gctggggtac tggtaatcac ggctcaccac
     2581 aaacttatgc agcgggttct ctgggacgtt ttggtaatga gatgagcggt tggtttgacc
     2641 tgaccttaaa tcagcgtgtt tataatcagg acggtaaaac ggcaaatgcg gtcgttacct
     2701 atgatggcaa cgtgggtgag cagtataacg atgcctggtt tggtgacagt gccaatgaaa
     2761 atatcatgca gttcagcgat atttacctga caacgcgagg ttttttaccc ttcgcaccag
     2821 aggcagactt ctgggtcggc aaacataaac tcccgcaata tgagatccag atgctggact
     2881 ggaaaacctt aaccacggat gtcgccgcgg gtgtggggat tgaaaactgg gcacttggtg
     2941 tagggctgtt tgatatgtcc ttaagccgtg atgatgtcga tgtttactct cgtgattttt
     3001 cgcgtactag ccagatgaat accaattctg tggatgttcg ttatcgcaat atcccgttat
     3061 gggatgatgc gacattatca ttaatggcta aatattccgc acctaataaa acggatcaac
     3121 aacaagataa cgaaaatgac aacagttatt ttgaaatgaa agatagctgg atgctgactt
     3181 ctgttttacg gcaaaacctg caacgcgata cgtttaatga atttacgtta caggttgcca
     3241 ataattccta tgccagcagt tttgccagtt tctcagatgc cagtaatacg atggcgcatg
     3301 gtcgctatta ctatggtgat cataccaatg ggatcgcctg gcgtttaatc tctcagggcg
     3361 agatgtatct tactgataat attattatgg ctaacgcgct tgtctattct cacggcgaag
     3421 atgtttatag ttatgaaagt ggtgctcata gtgattttga cagtattcgc actgtaataa
     3481 gaccggcctg atctggaata catggaatca gacggggctt gaattaggct ggtttaagca
     3541 acagaacaaa actcagcagg gtgtaacact aaatgaatcg gcttataaaa cgacactctg
     3601 gcatgcattg aaagtgggtg aaagcatttt aggttcacga ccagaaattc gcttctatgg
     3661 aacgtatatc aatattctgg ataacaaatt atcgaatttt aagtttaatg agaacagcaa
     3721 agacgagttt atggccggca tccaggcgga agtctggtgg taatctattt tagttaagga
     3781 gttgtgtatg tcaggattta aaaaaggttt tttatggggc ggcgcggtag ccgcgcatca
     3841 gttggaaggt ggctggaatg aaggaggaaa aggcatcagt atcgctgatg tgatgactgc
     3901 tggcgctcac ggggtgccgc gtgaagtgac agaaggcgtt atcgacgggc ttaattatcc
     3961 caatcatgaa gcaattgatt tttatcatcg ctataaaaca gatattcagt tatttgccga
     4021 gatgggattc aaatgctttc gaacttccat tgcctggaca cgaatctttc cgcaaggtga
     4081 cgaacaggag ccgaatgaag agggtttaca attttatgat gatctgttcg atgaatgcct
     4141 taagcaggga atggaacctg tggtgacgct ttcacatttt gagatgcctt atcatctggt
     4201 gacaaaatat ggtggctggc gaaaccgtaa actgatcgac ttttttatcc gcttcgcatc
     4261 aacggtcttc acgcgctata aagaaaaagt aaagtactgg atgacgttta acgaaatcaa
     4321 taatcaggtg aatttcagcg aaagcctgtg tccatttact aattccggta tcttgtattc
     4381 gccagaggaa gatatcaatg agcgcgaaca aataatgtac caggcggtac attacgagtt
     4441 agttgccagt gccctggcgg tacagactgg aaaatcgatt aatcctgaat ttaatatcgg
     4501 ctgcatgatc gccatgtgcc ccatctatcc tctgacgtgt gcacccaacg atatgatgat
     4561 ggcgacgaaa gcgatgcatc gtcgttactg gtttactgat gttcatgctc gtggatatta
     4621 tccgcaacat atgctgaatt actttgccag gaaaggattc aacctcgata tcacaccaga
     4681 agataacgcg attcttgcca gaggttgtgt cgactttatc ggttttagct actacatgtc
     4741 ttttacgaca caattttcgc ccgataaccc gcaactggat tatgttgaac cacgagattt
     4801 ggtcagcaac ccttatatcg atacatccga atggggatgg caaattgatc cggcagggct
     4861 acgttattca ctcaactggt tgtgggatca tttccagttg ccgctgttta ttgtcgaaaa
     4921 tggatttggt gcggttgacc agagacaagc tgacggcaca gtgaacgatc actatcgcat
     4981 tgattacttt gcttcccata ttcgtgaaat gaaaaaagcc gtagtcgaag atggtgttga
     5041 cttaattggc tacaccccgt ggggctgcat tgacctggtt tctgccggaa caggggaaat
     5101 gaaaaaacgc tacggaatga tttatgtcga taaagacaac gaagggaagg gaacgctgga
     5161 aaggatacgt aaagcgtcgt tttactggta tcgggatctc atcgccaaca atggcgaaaa
     5221 tatttgagac ttcagaaaga tattacggag atgtcaggct ctgtgatcag agcctgacaa
     5281 taactacata gcgtgttgat tataataggg cacgccgagc gcatccgact tatcactgcc
     5341 agtacccatg tgatttaaat caaacggcgg ttgttcctgc gatggaggag caaccaccac
     5401 attgccacag gaagtaacaa ccggctgcgt gggttgttcc gcaacgccct gcgcggaaaa
     5461 gagaataagc gcagctgcta ttgcggataa aagtggtttc atgattgcct cagtgacgtt
     5521 gtcacgttat caactagctg ttgtaatgat ttaatggatg taaaaagcgc gattcgccct
     5581 gcatattggt catccggtat ttatgcggcg gaacatcaaa gtaatttttg aaggttcggg
     5641 tcagagtttg ttgcgactcg aagccatatc gttctgccag atagagtatc ggctcgttac
     5701 tttccttcag cttttgcgcg atttccgtca tcttacggct gcggatgtat tggcctaatg
     5761 aatgaccggt ttctttttta aacatccgtt gcaggtgcca tttggagtaa cccgaacgct
     5821 ctgacacttt ctccagtgac agtggcgatt ccaggttgtc ctcgatccag tccaaaatgc
     5881 tatgaatggt aatagcgtca gtattgcgtc tggacatcgt catacctctt ttttgtttac
     5941 ggcagaactt tcttaagcaa atgctcaagt gttgccactt cgtccgccgt caggtttttt
     6001 gttaattctt gatgcaggtc ctggccaact aattgatggc attgttcgca tattgccgcg
     6061 ccgctggtgg taagttttac cagtacgccg cgcttatcat tcgggttcgg caaccgttca
     6121 acccagcctt tacagaccag gcgatccagc atacgggtca gtgctcccag gtcgaccgac
     6181 aacacttttt tcagttcaac cggagtaata cacgccgcgc agcggataga gcagagcacc
     6241 ttaaactgtg ccgcagtaat atccagcgga gacagatact cgttaagcag acgatctttc
     6301 ttctgattaa ccatatggat taagcgaccc aatggaataa tttcattgaa cagatcgctg
     6361 gtacttttca cattagttgc cctggcaagt aattagttgc aggggataat attgcccagg
     6421 caagtataag tcaaatgaat gaatgaccga tgccacgttt tgctaaatcg gttcaagttt
     6481 tggtcaatgc gatgcaaggt tagctaaaga gtgacacttt atccttgttt accccttata
     6541 cttttcgctg ataacccaga tacacaggat aacaaccacc aatgttagat ttgtttaaag
     6601 caattggcct ggggctggtg gtgttactgc cgttagctaa cccattaaca actgtcgcgt
     6661 tatttcttgg cctggcaggc aacatgaaca gtgccgaacg taatcgtcag tcgttgatgg
     6721 cctcggtgta cgtatttgcc atcatgatgg tggcgtatta cgccgggcaa ctggtgatgg
     6781 atacatttgg catttcgatt cccggtctgc gaattgcagg cggcttaatc gtcgcgttta
     6841 tcggttttcg gatgctcttt ccgcaacaga aagcgattga ttcaccggag gcgaaaagca
     6901 agtcggaaga gctggaagat gaacccagtg cccatattgc ctttgtgccg ctagcaatgc
     6961 caagtaccgc tggtccggga accatagcga tgattattag ctcggcgtcc acagtgcgtc
     7021 agagttcaac cttcgctgat tgggtactga tggttgcgcc gccgctgatc tttttcctgg
     7081 tggcggttat tttgtgggga agcctacgca gctccggcgc aattatgcgg ctggtgggca
     7141 agggtggcat tgaagcgatc tcccgtctga tgggattcct gctggtatgt atgggtgtgc
     7201 agtttattat taacggcatc ctggaaatca ttaaaacgta ccattaaaaa aggcccgaat
     7261 gggcctttca actattgcgt ctgttcttcg agtgtcactg gccagcggcg aaatataatg
     7321 attgaccata ttaacgcggc aaaagcaggc accgcgccca cataaccaat catcgacatt
     7381 gaccagtgca aactcacctg attacctacc aacgcacccg ccccgattcc aatattaaat
     7441 atgccggaga atagcgccat cgcgacgtcg gtagcatctg gtgccagcgc cagcacttta
     7501 acctgcatac caagcccgat gatcatcatc gcgatccccc agaaaatact cagcaccccg
     7561 aggtgtattt cactgttcgc cgcaggtagc agcagtgcca gacacaccag caacagcgca
     7621 attgccgtac tcaccaacgc agacgcatac tgattaccca gtttaccgaa aatcacgctg
     7681 ccaataatgc ccgcaccacc gagtaataac agtaatgccg tggcaaagtt ggcgctgaat
     7741 cccgcaatgt tttgcacaaa aggctcgata tagctgtatg ccgtgtaatg ggcggtgaca
     7801 accaccacag ttaacaaata aatgctcatc aatgccgggc ggcggaacaa tagcgggagg
     7861 cttttcagtg aaccggaatg ctcactgggc agtaagggaa gtaacttaat caggcacaaa
     7921 agggtgataa gcgccccaat accaatcgcg aagaaggtca ttcgccaacc gaaatactgg
     7981 cccacaatgc gcccgagagg taaacctaag accatcgcca gtgctgtacc ggtggcaatt
     8041 aaactcaatg cctgtgctcg cttcccggcc ggagccatac ggatcgccag agacgccgta
     8101 atcgaccaga aaatcgcatg tgcaaaagcc acaccaatgc gactgatcac cagaacggta
     8161 aagctccacg acaaaaacga cagtacgtgg ctggcaataa acaccacaaa caggcagatc
     8221 agtaatttgc gccgttcaac ctgactggtc attaacataa aaggcaatga cattagcgct
     8281 actacccatg cgtaaatggt caacatgatg ccgacctgag cggtttgcat gtgaaaactt
     8341 tgcgcaatgt cagagagcag gccaacaggg acaaattcgg tggtgttgaa gatgaaggcg
     8401 gcgactgcca gcgtaacgac ccgtagccac gccactttgc gggaaacagt gtttgttgtc
     8461 ataacagatt atcgggtgag ttgcgaaaaa gtgatgaaat gatcttaaaa cggttaatga
     8521 tgtgaataca atgcatttgt gatctggatc gcgttttccg tttctctgac gctgattctg
     8581 ttccgcgtca gagagtcggt aacatcacgg tttaatgacg ttcaggaagg tctggcgttt
     8641 tggcggttgc gggtataggt ggtctgacag gggagcgagg cctgtttttc accaggtctt
     8701 cgtgagtaac ttttggcggc gtttcatttt ttacaaacag tttgagggtc ctgcgcgcca
     8761 tatgtttgat aattttatca tcacttaatg atgctggcat caacacgtca ttcagatcac
     8821 tttcattggg attcataaat tggtgatagt gcaataacat attggcgaaa aataaaaaaa
     8881 agtcctgaaa atgaggagat tcttgttctg ctaaaaggga taaaaagcag atgcagactg
     8941 atcctaccca cgtaatatgg acacaggcct aagcgaggtt cttgttttca aattgttccg
     9001 gactgaggcc gccacaccaa ctgtgccgcc gccaccgatt gtaatcacat tcgatataat
     9061 taaacaccgt tgcccgcatt atttcccggc tgataaagtg ttctccatgg atacattcta
     9121 ctttcagcga atgaaagaag ctttccacgc aggcattatc gtagcagcaa ccttttgcgc
     9181 tcatacttcc acgcagatta tgccgcttca gttgcgcctg ataatctgct gaacagtact
     9241 ggcctccacg gtccgtgtga acgataacgt tccggggcct cttacgccg
//
LOCUS       DACAPB010000174         9193 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.174, whole genome shotgun sequence.
ACCESSION   DACAPB010000174 DACAPB010000000
VERSION     DACAPB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.174"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..261
                     /locus_tag="IT904_003800"
     CDS             <1..261
                     /locus_tag="IT904_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HAP3193516.1"
                     /translation="GEELQGPTVDGNELREETRYLNVDYAAVTGLLVQFARETDDRVT
                     ALEEENTTLRQNLATADTRISTLENQVSELVALVRQLTGSEH"
     gene            316..435
                     /locus_tag="IT904_003801"
     CDS             316..435
                     /locus_tag="IT904_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP3193517.1"
                     /translation="MEIATEEETLLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(533..1123)
                     /locus_tag="IT904_003802"
     CDS             complement(533..1123)
                     /locus_tag="IT904_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3193518.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDHLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(1440..1673)
                     /gene="ydfK"
                     /locus_tag="IT904_003803"
     CDS             complement(1440..1673)
                     /gene="ydfK"
                     /locus_tag="IT904_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HAP3193519.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            complement(1742..1855)
                     /locus_tag="IT904_003804"
     CDS             complement(1742..1855)
                     /locus_tag="IT904_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193520.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            2457..3740
                     /locus_tag="IT904_003805"
     CDS             2457..3740
                     /locus_tag="IT904_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP3193521.1"
                     /translation="MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIV
                     GAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGL
                     GAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
                     WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLA
                     TGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTYALMISSILGFMTIPFLGWLSDKI
                     GRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMA
                     EMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLIS
                     ALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            3829..5289
                     /locus_tag="IT904_003806"
                     /pseudo
     CDS             3829..5289
                     /locus_tag="IT904_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(5325..5528)
                     /gene="ydfZ"
                     /locus_tag="IT904_003807"
     CDS             complement(5325..5528)
                     /gene="ydfZ"
                     /locus_tag="IT904_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAP3193522.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(5705..6391)
                     /locus_tag="IT904_003808"
     CDS             complement(5705..6391)
                     /locus_tag="IT904_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3193523.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(6480..7226)
                     /gene="ydfG"
                     /locus_tag="IT904_003809"
     CDS             complement(6480..7226)
                     /gene="ydfG"
                     /locus_tag="IT904_003809"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAP3193524.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKVEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            7363..>9193
                     /gene="dcp"
                     /locus_tag="IT904_003810"
     CDS             7363..>9193
                     /gene="dcp"
                     /locus_tag="IT904_003810"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAP3193525.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAA"
BASE COUNT         2368 a         2016 c         2283 g         2526 t
ORIGIN      
        1 ggtgaagagt tgcaaggtcc gaccgttgac ggcaacgagc tacgcgaaga aacgcgctat
       61 cttaatgttg actacgccgc cgtgacgggc ttacttgttc agttcgcccg tgaaacagat
      121 gatcgcgtta ccgcgctgga agaggaaaac acaacgctac gtcaaaatct ggcaacagca
      181 gacacccgga tcagcactct ggaaaatcag gtaagcgaac tggttgcact tgtccggcag
      241 ttaacaggaa gcgaacattg atatccttca agctctgaag gaggctgttc ccggtacgtt
      301 cagactgttg ttgagctgga aatcgcaacg gaggaagaaa ccttgttgct ggaagcctgg
      361 aaaaagtatc gggtgttgct gaaccgtgtt gatacgtcaa ctgcacagga tattgaatgg
      421 ccagcactgc cgtagggtaa aacatataaa ttctataatt agatgtatct ttccatttac
      481 ggcaaggaag ggggcttgga agacgtaaag catctcacac cgagattatt ttttatatgt
      541 caggtgtctg aagttttgct ttggctctta aaatggtttg ccgcgaggtt ttgaattccc
      601 gggcaatggc acttatactt acacctgact taattcgttc gaataccacc tgtttctgtt
      661 cttcatttaa cacaggtggt cgaccaaaac gtttccctgc gccgcgggct cttactatcc
      721 cggaatgagt gcgttcaagt aaaaggtctc gttcaaattc agcgactgct gaaattacgt
      781 gcatcatcat ttttcctgtt ggactggtca ggtcaatgcc ccccaatgct aagcaatgca
      841 ctctgatacc tgtttcggtc agttgttcca ctgttttcct gatatccatt gcattacaac
      901 caaggtgatc cagttttgtc acaatcaatt gatcaccaca tttcaggcga gcaagcaacc
      961 ggttaaaacc aggacgctca ctggttgctg ctgagccgct aatgtgttct tcgattattt
     1021 gctgaggttt gattttaaaa cctgcacttt cgatttcccg gcgttgattt tcggtggtct
     1081 gatccagcgt tgatatccga cagtaagcaa aaattcgaga catagtgaga ctctatacga
     1141 aattggtgtt catatcataa tgcatctcag aaaataattt tgattatttt tgtgcatatt
     1201 tgtatgtaca cgttcgaaaa taaacgaatg cgtatgcaac cccgtaactt tggtgagacc
     1261 caaaatcgat tttgtgaaaa atggctttaa ctcggtttgt ttttcgagtt ccgggcggac
     1321 tcaaggaaga agaatagtgt tgcgtgttat tttaaccaga tttcaagttg tttggtcgtg
     1381 gaaaagtgga gcaaaatgtt gttaaagtgg aaaaatgata aaaaagtaag tttattatat
     1441 tacattttac catttaaatt ttggttgtct ttaagaactg atatcgctgt ttgtaataat
     1501 tctttgttat ccagccatga ttttttcttt atgtttcctt caatgtaatc aagcaatgtt
     1561 ctggtattga taggtcttcc ctgttttgct acttccacta cagcatcccc taggataatt
     1621 cttacttcag gaagctgcgc agggaaccac tttagggtgt cttttgattt catgaagata
     1681 ttccttaaaa tattattgat tttcattgcg atattgtatg tctgattcag gatatgttga
     1741 cttatacatc ggttttgtct gggttattgg atatgccaat ccctaatttt attagggcat
     1801 gactaaaaat gctgaatatg ataaggagag aagtgattat cagtatgctg ttcatatagc
     1861 ctcgaattag taatgtgtta tatatgatat agttgacaat tttatcttgg gtgttcttaa
     1921 agttcgtaga taaacattgt cgtttcaggt atacaggaat gctaacaggt ggcggcaaaa
     1981 atcaggcggt ttatggcgca atctgaagtg gcaactgcaa actatcttat gcagagactc
     2041 tacacggatt gggtttaaaa gtatacatag ataacagttt ttatctgaag aagaaaaata
     2101 tcaaggtgat atagcctata tgcctttgat gtggaggaat gaatgtgatg ggagtgatgt
     2161 atctgaatag ttgaaaaacc gcagacacac cttatgcaag aacgtgctgc ggttggctgg
     2221 taaatttttc gatagtgcga gtattgaatg atttccagcc gttattgatt ttacgcgtaa
     2281 atccatgaaa aaactactta tctgttgggg agttttttgg ggcatatatg ggacagaaat
     2341 aggccccaga tagacactga gatcgaactt aggatgcttt taaaaaaatg caactatctg
     2401 aaaaaaccta gaaaacgcca aggaaaccac aggatgggaa aaaacacctg tgaattatgg
     2461 atttccagtt atattcgctc ggcgcagcgt tagtgtttca tgaaatattt tttcctgaat
     2521 catcaacggc aatggcgtta attctggcaa tgggaaccta cggtgcaggt tatgtggcgc
     2581 gtattgtcgg agcatttatt ttcggcaaaa tgggcgacag aatagggcgt aaaaaagtgc
     2641 tctttattac catcaccatg atggggatct gtaccacctt aattggtgtg ttaccgacct
     2701 atgcacagat tggtgttttt gcccccatct tgctggtgac gctgcgtatt attcagggat
     2761 tgggtgcagg tgcggaaatt tccggtgccg gtacgatgct ggcggaatat gcgccaaaag
     2821 gtaagcgcgg aattatctcc tcatttgtgg ctatgggaac taactgcgga actttaagcg
     2881 caacggcaat ctgggccttt atgttcttca ttctcagtaa agaggaactg ctggcgtggg
     2941 gatggcgtat accgttcctg gcgagcgttg tcgtgatggt ctttgctatc tggttgcgta
     3001 tgaatctgaa agaaagcccg gtttttgaga aggttaacga cagcaaccaa ccgacagcaa
     3061 aacctgcacc tgctggtagc atgttccaga gcaaatcctt ctggctggca acagggctgc
     3121 gttttggtca ggcgggtaac tcaggtttaa ttcagacttt ccttgcgggc tatttagtgc
     3181 agacgttatt gtttaacaaa gcaattccaa catatgcatt gatgatcagt tcgattctcg
     3241 gctttatgac cattccgttc cttggttggt tatccgataa aattggtcgc cggatcccgt
     3301 atattattat gaatacctcc gcgattgtgc tggcatggcc aatgctttct atcatcgtag
     3361 ataaaagcta tgccccgagc accattatgg ttgcactgat tgtgattcat aactgtgcgg
     3421 tgctgggatt atttgctctg gaaaatatta ccatggcaga aatgttcggc tgtaaaaacc
     3481 gctttacccg gatggctatt tctaaagaaa ttggtggtct tatcgcttcc ggttttggtc
     3541 ctatcctggc gggtattttc tgcaccatga cggaatcctg gtatccgatc gccattatga
     3601 tcatggcata ttcagtgatt ggtttaatct ctgcgctgaa aatgccagaa gtgaaagacc
     3661 gtgatttaag tgcgctggaa gacgccgcgg aagatcaacc gcgtgttgta agagctgcgc
     3721 aaccttccag aagtctgtaa atcctcaatc ccttctctta ccggagaggg gatttttatt
     3781 catataacaa aacatgtagc ttgccatatt tatatttaag gaattgttat gggaaataat
     3841 ttgttatcag caaaagcgac actccctgtt tatgatcgta ataacctggc cccaagaatt
     3901 gttcatttag gctttggtgc atttcaccgt gcgcatcagg gcgtgtatgc cgatattctt
     3961 gctacagaac atttcagtga ctggggatat tatgaggtca acttaatcgg cggcgaacag
     4021 caaattgccg atttacagca gcaagataat ctttataccg ttgcggaaat gtcggccgat
     4081 gcgtggacgg ctcgcgtcgt tggtgtcgtt aaaaaagcct tgcacgtaca gatggatggc
     4141 ttagaaaccg tgttggctgc gatgtgtgaa ccgcaaatcg cgattgtctc tctgacaatc
     4201 accgaaaaag ggtatttcca ctctccggcg accggacagt taatgctcga tcacccgatg
     4261 gtcgctgccg acgtgcaaaa tccccaccag ccgaaaacag caacaggggt gattgttgag
     4321 gcgctggctc gccgtaaagc ggcaggactt cccgcattta ccgtcatgtc atgtgacaac
     4381 atgccagaaa acggtcatgt tatgcgtgac gttgtcactt cctacgcgca agccgttgat
     4441 gtaaaactgg cacaatggat cgaagataac gtgactttcc catcaacaat ggtggaccgt
     4501 attgtgcccg cagtgacaga ggatacgctg gcgaaaatcg aacaacttac cggtgtgcgc
     4561 gatcctgctg gcgttgcctg tgaacctttc cgctagtggg taatagaaga taattttgtt
     4621 gccggacgtc cggaatggga aaaagcggga gcagaactgg ttagcgatgt actgccttat
     4681 gaagagatga agttgcgcat gctcaacggc agtcattcat tcctggcgta tttgggttat
     4741 cttgccggat atcagcacat taatgactgt atggaagatg aacattatcg tcatgcggcg
     4801 tatggcttga tgttgcagga acaagcgccg acgctgaaag tgcagggcgt tgatttgcag
     4861 gattacgcta accgattaat tgcacgctat agcaacccgg cgttacgtca tcgaacctgg
     4921 cagattgcga tggatggcag ccagaaattg ccacagcgga tgttggattc tgttcgctgg
     4981 catctgacgc atgacagcaa gttcgatctg ctggcgctgg gcgtcgcggg ttggatgcgt
     5041 tatgtcggtg gtgttgatga acaggaaaat ccgatagaaa tcagtgaccc actgttacct
     5101 gttattcaga aggctgtaca aagtagtgcc gaagggaaag cgcgcgtcca gtcattgctg
     5161 gcgattaagg cgatctttgg tgatgatttg ccagccaata gcttgtttac tgcaaaagtg
     5221 acggaagcgt acttgtcttt attagcgcat ggcgcgaaag cgaccgtggc aaaatattcc
     5281 gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac acgcttagtt catgccgaga
     5341 cgaatcaggt ccagtggtgc cagtttctct tcacaacctt caacaactac ggtttttccg
     5401 cggcggattt gctcagcggt cagaccttca gtatcaatcg acaggatctt gccagtgtga
     5461 ccggtgccgc taaccataac acggctgcca gtggtgattg cgttacggtt acgatcgtag
     5521 gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa cgggcgcaat
     5581 ataaatactc ctgtcgagtg aaatattttt tgtttttgat caagctcaca tcttttttga
     5641 tgcatgcatg agccataagg cgggggaaaa gactctcacc ccggatggga tgagaggaaa
     5701 tgaattactc ttcgctaaac cagtcgctat tttcctggcg gatctggcgc acggattcgc
     5761 tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca cgtttttgca
     5821 gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa acgtggtcga
     5881 aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta tcccacgcca
     5941 gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt tcaaaaaaat
     6001 catccagttg cttgcgctca atggcaatgc gttgctggtg aagattttgt tccagttggt
     6061 agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac tcaatggcct
     6121 gacggataaa actgccgttg cgcacctggg ccatggaaat tttgttgacg tagctgccac
     6181 gctgcggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct tcacgaaccg
     6241 gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt gtgcctggtg
     6301 caatcaggca atggacaatg tcgcgacgaa gaatacgata aatctgctga ttgacaggct
     6361 gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg agttaactgc
     6421 atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg gtataaaaat
     6481 tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat ttccagggta
     6541 ttgatattga cgtgagcagg cagcgttgac acccaccaga cggcttcgct gacatcttct
     6601 ggcgtcaatg caacggtatt ttgataggtt ttttccactt taccgtcatc gcctttaaag
     6661 cggacattgg aaaactcggt gccacccacc agacccggct cgatgtcggt gacgcgtacc
     6721 gccgtaccat gcagatccgt acgcagattc aggctaaact gacgaacaaa cgctttcgtc
     6781 gcaccgtaaa cgttaccacc ggcatacggc cagctacctg ccgttgagcc aatgttaata
     6841 atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat atataccagg
     6901 cctttattgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt atgcgcaggc
     6961 tccatgccca acgccaggcc ggcattattt accaggatat caatattgct ccactcggca
     7021 gggagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag ttgggcgata
     7081 tacagattat ctcccagttc gtcttttaac tcctgcaacc gttcctgacg acggccagtg
     7141 gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc aaaacctgcc
     7201 gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt gtgtcaataa
     7261 catagcacgg gatgcttaag agcgggtaat actggattgc aagattgcac tccgttatca
     7321 gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac aatgaatcct
     7381 ttccttgtgc aaagcacact gccgtatctg gctcctcatt ttgatcaaat tgccaatcat
     7441 cactatcgcc cggcattcga tgagggaatg cagcaaaagc gggcagaaat tgctgccatc
     7501 gcgcttaacc cgcaaacgcc tgatttcaac aatactattc tggcactgga acaaagcgga
     7561 gaattactta cccgcgttac cagcgtcttt tttgcgatga ctgcggcgca taccaatgat
     7621 gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc taatgatatc
     7681 tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcagcgccg tgaatccctg
     7741 gggcttgata gtgaatccat ccgcctggtg gaggtgattc atcaacgttt tgtccttgcc
     7801 ggagccaaac ttgcgcaagc tgataaagca aaattaaaag tactgaatac agaagctgcg
     7861 acactgacca gtcagtttaa ccagcgatta ctggcagcaa ataaatccgg cggtctggtt
     7921 gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag agattgcgct ggcggcagag
     7981 gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgctgctgaa taccacccaa
     8041 caaccggcgc ttgccgagat gcgcgatcgt gcgacgcgtg aaaaactgtt tattgcgggc
     8101 tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg ctatcattca acgtctggta
     8161 gagattcgcg cacagcaggc gaaactgctt ggttttcctc attatgccgc atggaaaatc
     8221 gccgatcaga tggcaaaaac acctgaagca gcacttaact ttatgcggga aattgttcca
     8281 gcggcgcgtc aacgtgcgag cgatgaatta gcctccatac aggcggttat tgataagcag
     8341 cagggcgggt ttagcgcgca gccgtgggac tgggcatttt atgccgaaca ggtacggcgg
     8401 gagaaatttg atcttgatga ggcgcagctc aagccatatt ttgaattaaa cacggtgttg
     8461 aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt cgaacgtttt
     8521 gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca taatggcgtg
     8581 ggactggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg cggtgcatgg
     8641 atgggcaatt ttgttgagca atcaacgctt aatgaaacgc atccggtaat ttataacgtc
     8701 tgcaattatc agaaacccgc tgccggtgag cctgcgttgt tactctggga tgatgtaata
     8761 accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca gcgttatgcc
     8821 acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca aatcaacgaa
     8881 cactgggcaa cgcatccgca ggtattcgct cgctacgccc ggcattatca gagcggggca
     8941 gcaatgcctg acgaactgca acagaaaatg cgtaatgcca gcctgttcaa caaagggtat
     9001 gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg cctggaagaa
     9061 aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc ggaaaatatg
     9121 gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat ttttggtggc
     9181 ggatatgctg cag
//
LOCUS       DACAPB010000175         9192 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.175, whole genome shotgun sequence.
ACCESSION   DACAPB010000175 DACAPB010000000
VERSION     DACAPB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.175"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..234)
                     /locus_tag="IT904_003811"
     CDS             complement(<1..234)
                     /locus_tag="IT904_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="low- specificity; catalyzes the formation of
                     acetaldehyde and glycine from L-threonine; acts on
                     L-threonine, L-allo-threonine, L-threo-phenylserine, and
                     L-erythro-phenylserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAP3193526.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIV"
     gene            complement(271..1989)
                     /gene="poxB"
                     /locus_tag="IT904_003812"
     CDS             complement(271..1989)
                     /gene="poxB"
                     /locus_tag="IT904_003812"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAP3193527.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(2122..3090)
                     /gene="hcr"
                     /locus_tag="IT904_003813"
     CDS             complement(2122..3090)
                     /gene="hcr"
                     /locus_tag="IT904_003813"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAP3193528.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(3102..4754)
                     /gene="hcp"
                     /locus_tag="IT904_003814"
                     /gene_synonym="priS"
     CDS             complement(3102..4754)
                     /gene="hcp"
                     /locus_tag="IT904_003814"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAP3193529.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(4898..5797)
                     /gene="lysO"
                     /locus_tag="IT904_003815"
     CDS             complement(4898..5797)
                     /gene="lysO"
                     /locus_tag="IT904_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAP3193530.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(6292..6987)
                     /gene="aqpZ"
                     /locus_tag="IT904_003816"
                     /pseudo
     CDS             complement(6292..6987)
                     /gene="aqpZ"
                     /locus_tag="IT904_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            7412..9070
                     /locus_tag="IT904_003817"
     CDS             7412..9070
                     /locus_tag="IT904_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAP3193531.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRMAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(9067..>9192)
                     /locus_tag="IT904_003818"
     CDS             complement(9067..>9192)
                     /locus_tag="IT904_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAP3193532.1"
                     /translation="LPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
BASE COUNT         2286 a         2388 c         2398 g         2120 t
ORIGIN      
        1 gacaatatac tcttcgccac gttcgcagtg actgagcaga gcgaccaggt tggcctgagt
       61 gccggtaggc agaaaaatgg cggcttcttt accggaaagc tctgctgcat agtcctgcag
      121 agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca accggggcgg ccatcatcgc
      181 ttcgagcatg gcgcggctcg gtcgggtaac ggtatcactg cgtaaatcaa tcatggcatg
      241 tccttattat gacgggaaat gccacccttt ttaccttagc cagttcgttt tcgccagttc
      301 gatcacttca tcgccgcgtc cgctgatgat tgcgcgcaac atatacaagc taaatccttt
      361 ggcctgttcg agtttgatct gcggtggaat ggctaactct tctttggcga ccaccacatc
      421 caccagcacc ggaccgtcga tggagaaggc gcgttgcagg gcttcatcaa cttcagacgc
      481 tttttctaca cggatacccg taatgccgca cgcttcggca atgcgggcaa agtttgtgtc
      541 gtgtagttcg gtgccgtcag tcaaatagcc accagctttc atctccatcg ccacaaagcc
      601 cagcacgctg ttgttaaaga cgacaatttt cactggcagt ttcatctgca ctactgagag
      661 gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac atggcgacca cctgacgttc
      721 tggctctgtc gcctgcgcac ccagcgcctg cggcatggcg ttagccatcg aaccgtggtt
      781 aaacgaacct aacaggcgac gcttgccgtt catttttaga taacgcgctg cccatactgt
      841 tggcgtgccg acgtcacagg taaagattgc gtcatcggcg gcaaaatgac taatttgctg
      901 cgccagatat tgcgggtgaa tggctttctc gctcggttta gctaaatcgt ccagcccttt
      961 gcgggcatcg cggtaatctt ccagcgcttt atccagaaat ttgcgatcgg ctttttcttc
     1021 caccagtggc agcaatgcac gcagggttga tttgatatcg ccgaccagcg ccatatctac
     1081 cttgctatgc gcgccgatgc tggctggatt gatatcaatc tgaatgattt tggcatcggt
     1141 cgggtagaag gcgcggtagg gaaattgcgt gccgagcagc actaacgtat cggcattcat
     1201 catagtgtga aaacctgacg agaagccgat taacccggtc attccaacat catacggatt
     1261 atcgtattcg acatgttctt taccgcgcag ggcatgaaca ataggtgctt taattttccc
     1321 ggcaaactca actaactctt tatgcgcccc cgcgcagccg ctgccacaca tcagggcgat
     1381 attgctggaa taacgcagca gttgcgccag tttgcgtaac tcttcttctt ccggcgtcac
     1441 aactggctgt ggcgcatgat accagtgggt agttgctcct tctggcgcag gttttaacgc
     1501 cacgtcaccc ggtaacacaa caacggaaac gccacggtta agcactgctt tgcgcatagc
     1561 aattgccagc acttgtggga tctgctccgg gctggaaacc agctcgcaat agtgactaca
     1621 ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag ccgctgccaa tttcgctgga
     1681 gggaatatga gcggcaatcg ccagtaccgg aacgtgattg cggtggcaat cgaacaggcc
     1741 gttgattaag tgcaggttgc cggggccgca cgatccggca cagaccgcca gttctccgct
     1801 aagttgtgct tcagcgccag cggcaaaggc cgccacttct tcgtggcggg tggacatcca
     1861 ctcgatggtg cccatgcgat taagactgtc actaagaccg ttcagagagt cgcctgtgac
     1921 tccccagatg cgtttcaccc ctgccgattc gagtgttttg gcgatataag ctgcaaccgt
     1981 ttgtttcatg gttctccatc tcctgaatgt gataacggta acaagtttag ttcatctgac
     2041 ggagggggaa gggatgggag agaaaggagg cactaacggt taaatagccc gatgaaagga
     2101 atatcatcgg gcataaggcg attatgcgag aaccaaatcc ccctgcggat ggcaggagca
     2161 ggccagtacg taaccttcag cgatttcggc gtcggtcagc gtcattgtgc tgctcaccgt
     2221 atattcaccg gaaaccactt ttgtcttaca gcagccgcaa acacccgcac ggcaggcagc
     2281 gacaaccgga acgttattgc tttccagcgc ctccagtagc gtggtgccaa ccggggcgta
     2341 aaattctcgt gccggttgca gtttggtgaa tttcagaccg ctggtcgccg cttctgctac
     2401 tggggtgaag aatttctctt taaagaaacg cgtcacgccg agcgctttca cttcctgctc
     2461 tacccaatcc atatacggag ccgggccgca ggtcatcacg gtacgtgaag ctaagtcagg
     2521 tacacctgcc agcagttcgc gagtgagacg accagcgata aagccttcgg taacgttatt
     2581 ttctgccacc agcgttaccg gatagttacg ccactcatcg gcgaaaataa catcctgcgg
     2641 cgtacgcacg ttgtagatca cctgtacatc ggcctgtgga cagttcttcg ccagccagcg
     2701 acgcatcgac ataatcggcg tgacgccgca gcctgccgcc agcaacagga atttatcttc
     2761 tgctttatcg tcgcaggtaa attcccccat cgcgtccgaa agccagagat aatcaccgcg
     2821 ttttacatcg cgcgtcagcc actgggagcc gacaccgtca tcaatccgcc gcacggtcag
     2881 ggtgatatat tcactcacgc ctggcgtgga ggaaatggtg taagcacgca gcgtttccgc
     2941 tgagtttcgc acgctgacca gtgcatattg cccggcgcga tatgggtagt aatcgtggca
     3001 aatcagggaa atcgtccaca catccggcgt ttcttgcgta atgtgatgaa cctgcatccg
     3061 ccacgggcat tgattcgttg gcatcgtcat cgacaaactc cttacgcgct caacagttgc
     3121 ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga atttctcgtt cagcaccgcc
     3181 agcaggtctg gtgtcaggaa accaggtgca gtcgggccgg tgacgatatt tttcacgccc
     3241 agagaaagca gcgtcagcag aatgacgatc gctttctgtt caaaccagga gagcaccagc
     3301 gacagcggca gatcgttcac accacagccc agtttctctg ccagagtgac agccagaata
     3361 atcgctgagt aagcatcgtt acattgacct gcatctacca gacgcggcag accttcgata
     3421 tcgccaaact caagtttgtt aaagcgatat ttaccacagg cgagggtcag gatcaggcag
     3481 tcatccggca cgctggtggc gaaatcggtg aagtagtggc gctcgccgcg tgcgccgtca
     3541 cagccaccaa gcaggaagat atgacgcagt ttttcacggc tcaccagatc aatcagcgta
     3601 tcagcagcgc caagcagcgt ctggcgacca aaacccacgg tgataaggtg cggaatttcg
     3661 ctgtacggga agcctgccat ctgttgcgcc tgggtgataa ccgcagagaa atcatcaccg
     3721 tccagatgac gcacgccagg ccagccaaca atgctgcggg tccagatacg atcgtcataa
     3781 gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca cgatggggcc agggaaacga
     3841 gcgaactcca cttgctgatt ctgccagccg ctgccgtagt taccgaccag atgcttgaat
     3901 ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc cgtgggtgta gacattaacg
     3961 cccgtgcctt cggtctgttc cagcaggttg tagagatctt tgagatcgtg accggaaatc
     4021 aggatgcatt tacccgccgt cgctttgacg ttgacctggg ttggcgtcgg gtgaccgtat
     4081 ttaccggttt cgcctgcatc cagaatgctc atcactttga agttcatctg gccgatttcc
     4141 attgaacact caagaagcgc gttcatatcg gcaggccagg tccccagcca cgccatgatt
     4201 ttatgatact gggcataaat atcgttgtcg tattgaccga gaacatgcgc gtgttccata
     4261 taggccgccg cacctttcag gccatacagg cacagcagac gcaggccgag aatgttttcg
     4321 ccaatcgccg ctttatcttt gttaggggta aattctgctg cctgacgttg cagctcgccg
     4381 agatcgtcgc tcaccagttg caggtcagcc atcgggttat cgacgcgcgc gttggcatct
     4441 acagccaggc attgcgcttt cagcgcctcg cgcagggcaa tcgcttcacg agcgtagccg
     4501 acaatacgcg gagaatcgaa gttaacgttg gtcagggttg agaaaaaggc acgtggcgcg
     4561 aagctgtcaa catcgtggtt gatgatgccg tattcacgcg cttttaccgc ccaggcagaa
     4621 agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag aagtttccgc cgttttacca
     4681 cacatcccct gcgcgtatga gcagccgttt cctgccggag tacggatagt ttgttcacat
     4741 tgcacacaaa acatgatcac accttttaaa gttatattta atatacatgt ttaaggttaa
     4801 gacgcttaac gcggggataa aagggatttt tcatgcaact ttaagggaga ttgatttagc
     4861 gcaattttgg cggcagggat ctaccgccag agaggtatta cgcagagaaa aaggcgatga
     4921 ggatcggcac taacaggcta agaataaaac cgtgaacaat tgccgccggg accatatcca
     4981 gcccgccagt acgttgaaga acgggcaggg tgaaatccat tgatgtggca ccgcataagc
     5041 ccagtgcagt agagcggctg cggcgaatca gcccagggat caacataata gcaatcagtt
     5101 cacgggccag atcattaaaa aacgccgcgc tcccgattac cgggccaaaa gattcggtca
     5161 ataaaatacc ggaaagagaa taccagccga aaccggaggc cattgccagc gcggtattga
     5221 tggggagatc aagaataaag gcgttaatta aaccaccaat taatgaactg gcaaccacca
     5281 ccacggcgac aatcattcct cggcgattaa ggacaatctg ctttaaggtc atgccattat
     5341 tgcgcaactg aataccgacg aggaaaagta gcagaattaa cgtgtattcg ctggcttcgg
     5401 tcgcgtgttg taagaaagcc agtccactca gaccaatggc aaaaccaatc actactacgc
     5461 cgcacagttt tagcgactcc agcgccatcg caatacgcga cgggagtttt tcttgctgat
     5521 ggtgattgcg ccatggcagg cctcgctcca gccacatcag ggcggcaata ttacatagta
     5581 aaataacggt aatactgaca gcagaataat gcagaatcgc caacaggtta ctggcgaggt
     5641 tatcgagaaa cgccagactg atacccataa aaaagagaat aaggtaaacc atccagctta
     5701 atagctgatt aataactttt aatgcagctt tttggcgaag cggaatgagg taacccacaa
     5761 tcaggggaac cagaatgatt aacagcccag aaaacatgaa aacccagtcc ttgcaaagat
     5821 gaagtcgaaa tgcgcgatga cacactactg aaagcggaag gacgagtaaa gttgcaatta
     5881 aaaggaaatg ttatgcataa ggagcagtag agtattcgtt ttcatttaaa gatattcttg
     5941 cgctttaatt acaaactgca ccgatgttgg tggcgtcaaa atcgccgagg cgttccctga
     6001 aggccggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg
     6061 cgcccgaccc ggtagccgta agggataagt cgagggcacc gcgcagcggc gattttgttc
     6121 gccagagccc gggggtgcag ggggaggagg cgattgtcct ccccctgcgc gctccttgcg
     6181 ccagtggcaa tatgtggctt agctcatgaa aggagcgcaa caagatgatg aatcaacata
     6241 taacaacatc ttaaaaaaag gcctgacatt acgccaggcc ttctgcgtta attaatcacg
     6301 cttttccagc agggtccggt aaatcagatc accgataatg ccgccgacaa ttggcaccac
     6361 ccagaagaac cacagttgtt ctaatgccca gccgccctgg aagatagcaa ccgcggtgct
     6421 gcgcgccggg ttaacagaag tgttagtcac cggaatactg attaagtgaa tcagggttaa
     6481 ggccagacca atagcgatcg gcgcaaaacc tgccggagcg aatttgtcgg ttgcgccgtg
     6541 gatcaccaac aggaaacctg cactcaatac cagttcaact accagcgcgg aaagcatgga
     6601 ataaccgcct ggtgaatgct cgccataacc gttagaagca aaaccgctgg ctgccgcgtc
     6661 aaaacccgtt ttaccactgg caattaaata cagcagcgct gccgcaacaa taccgccgac
     6721 aacctgggca attacgtagc caacgactta ttttgccgga aaacgtccgc cagcccataa
     6781 accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag caaaggccat
     6841 cgtcagaacg gtcagaccga acgccaacgc cacgccggca aaaccaatgc ctaattccgg
     6901 gaagcctgcg gccagtacag cactaccaca gccaccaaaa acaagccaga aagtactaaa
     6961 acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac cctgtgaaaa
     7021 atatggtcgt tttatagggc cgtcgtaaaa agtgacgacg gaaataatgc gcggctattt
     7081 taaaaacgaa ggcgagtcat tcaccagata aataaatcca gtaaatttga tttagggcaa
     7141 cagcgggttg ccccatatag tcgtttgtct gattgacagt gtagtgcaag caaaagattt
     7201 aatcctttag gcgtaataaa aaataattta tcatgctaat tatttgattt tgttgttttt
     7261 gcagacttat cagcaagagg gagtataacg cgattattcg ctcatttttc agacatttgc
     7321 catgcttaaa tgtgatgtca tcacgtatta gcaaggcctt tcccgttata ctgccagcgt
     7381 aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg aaattgtggg
     7441 ttttcgcggt atcaaccgtt tgtcgttgat gctggaacaa aacaacgtcc tgattgggga
     7501 gaacgcgtgg ggtaaatcca gcttgctgga cgccttaact ctgctgctat cgccagaatc
     7561 agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata tcaacgggcg
     7621 agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc gacatcgggt
     7681 tcgccgttat cggccgctgg aagcgtgctg gacgccatgc accgatggct atcaccgtat
     7741 tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac tgcgcagttt
     7801 tctcgataaa gacggacatc cgattgatgt cgaggatatt aacgatcagg cacgccatct
     7861 ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc gtattcgtaa
     7921 cggcacggtg ccaaatgtcc ctaatgtgga agtcaccgcg cgccagctcg atttcctcgc
     7981 ccgtgagtta tcctcacatc cgcaaaatct ctctgatggg cagattcgtc agggactttc
     8041 cgcaatggta cagctgcttg agcattattt ctctgagcag ggggccggac aggcgcgata
     8101 tcgtttaatg cggcggcgag ccagcaatga gcaacgaagc tggcgctatc tggatatcat
     8161 caaccggatg attgaccgac ctggtgggcg ctcgtatcgg gttattttgc tcggcctgtt
     8221 tgctactttg ttgcaggcaa aaggcacatt gcgactggat aaagacgccc gtccattgtt
     8281 gctgatcgaa gatccagaaa cccgtttaca ccccattatg ctttcagttg cctggcatct
     8341 gttgaatctt ctgccattgc agcgcattgc caccaccaac tcgggtgagt tgctttcgtt
     8401 aacgccggta gagcatgttt gccgactggt acgtgagtcc tcgcgcgttg ccgcctggcg
     8461 tctggggccg agtggcttga gtaccgaaga tagccgacgc atatcctttc acattcgttt
     8521 taaccgtccg tcatcgctgt ttgcacgctg ctggttgctg gtggaagggg aaacggaaac
     8581 ctgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg aagggatcaa
     8641 ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc gccgaatggg
     8701 gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg ccgctacggt
     8761 acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg cgttaccggc
     8821 gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc accgcatggc
     8881 gcaaatcccg gaaaatgtac cgatgaatct acgcaaaatt atctcgaaag cgatccatcg
     8941 ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gcaggacgtc gtggtgtgga
     9001 ctccgtaccg acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg cgcgcggtcg
     9061 cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga gcatctcata
     9121 gcgccgacgg tattcagccc gttttttact ggcgatttcg gcaatctctt ttcgtgctat
     9181 ctgtgctgga ag
//
LOCUS       DACAPB010000176         9123 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.176, whole genome shotgun sequence.
ACCESSION   DACAPB010000176 DACAPB010000000
VERSION     DACAPB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.176"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            695..1912
                     /locus_tag="IT904_003819"
     CDS             695..1912
                     /locus_tag="IT904_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP3193533.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1996..2595
                     /gene="yfbR"
                     /locus_tag="IT904_003820"
     CDS             1996..2595
                     /gene="yfbR"
                     /locus_tag="IT904_003820"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAP3193534.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2654..4486)
                     /locus_tag="IT904_003821"
     CDS             complement(2654..4486)
                     /locus_tag="IT904_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAP3193535.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMIVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4573..5223)
                     /gene="hxpA"
                     /locus_tag="IT904_003822"
     CDS             complement(4573..5223)
                     /gene="hxpA"
                     /locus_tag="IT904_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAP3193536.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEVTETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(5234..5728)
                     /locus_tag="IT904_003823"
     CDS             complement(5234..5728)
                     /locus_tag="IT904_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP3193537.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5811..6266)
                     /locus_tag="IT904_003824"
     CDS             complement(5811..6266)
                     /locus_tag="IT904_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAP3193538.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6604..7806
                     /gene="ackA"
                     /locus_tag="IT904_003825"
     CDS             6604..7806
                     /gene="ackA"
                     /locus_tag="IT904_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP3193539.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7881..>9123
                     /gene="pta"
                     /locus_tag="IT904_003826"
     CDS             7881..>9123
                     /gene="pta"
                     /locus_tag="IT904_003826"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP3193540.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDE"
BASE COUNT         2433 a         2455 c         2146 g         2089 t
ORIGIN      
        1 actcttttct atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc
       61 actctcgtgc agcatcgctg gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc
      121 atcaagagca gggatccgtc atttatgcga gcattttacc ccaaaaacct tttatttata
      181 agggtcattg cgaattatct gatgcaaagt tatgctatgt taggcaaagt aatcttctat
      241 tattaataag cacatcaaaa cctttttgaa tattaaataa taattaatta gcatcatcct
      301 cattcattaa ttccgcttaa caatagtttc acaccttgcc cccattacga cacctcggaa
      361 atcatcacgc tgttaaactc tccgtaatgc ggaaatatca tcatcgcgtt tcattagtga
      421 attcttaaat gaggcatttt cacacaatta tcttacagat aaaaaaacca gacttacaat
      481 taagaatcag aacaattcac tatataacat tgcatgtaaa gcatatacac ctcattattt
      541 tgtcattatt aagttattaa cagcacaatc gagccttccc ctctggcaaa atcttattct
      601 gcagaccttc aaaacaccgt cctgggggag tacattgttc taagctgact tccacggcag
      661 ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa
      721 attagagaat gtctgttatg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga
      781 agaagaaggt aacaaggtac tgaaactgaa catcggcaac ccagccccgt tcggttttga
      841 cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc aagggtattg
      901 cgattccaaa ggtctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg
      961 catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg agcttatcgt
     1021 tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg ttggttcctg caccagatta
     1081 cccactatgg accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt atctttgcga
     1141 tgaatcctct gactggttcc cggacctcga tgatattcgc gctaaaatta cgcctcgtac
     1201 gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct
     1261 tttaatggag attgtggaga ttgcacgtca gcataatctc attatcttcg ccgatgaaat
     1321 ttatgacaaa attctctacg acgacgctga gcatcactca attgcaccgc tggcacctga
     1381 cctgctgacc attaccttta acggactgtc gaaaacgtac cgcgttgcag gcttccgtca
     1441 ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga
     1501 aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc
     1561 gctaggtggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca
     1621 gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc gtttcctgcg tgaaacctcg
     1681 tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc tttaacattc acgacgatca
     1741 gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag ggacggcatt
     1801 caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga
     1861 gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt
     1921 tcactgccgg agattgcatc cggcagcgtt atcccgccac aatgacctga tgatgtcatc
     1981 atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc
     2041 attaaccgct ggccgctcat gcgcaacgtg cggacggaaa atgtgtccga acacagtttg
     2101 caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcgaaaatt tggcggtaat
     2161 gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga agtgctcacc
     2221 ggcgatctcc ctactccggt gaaatacttc aattcgcaaa tcgctcagga gtacaaggct
     2281 attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct gcgggatatc
     2341 tttgcgccgt taattgacga gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag
     2401 gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa
     2461 ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag ccaggagatg
     2521 gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga gattagccag
     2581 gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg ccggatgcga
     2641 catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca
     2701 cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa tttcccggac
     2761 ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg gatgctgcca
     2821 tcgccacgat catcgcgaat ggataaggcg acacccccat cgttttggca gcagccagcg
     2881 caatcggagc catcaacacc gccgtcgcgg tattagagat aaatagccca ataaccgccg
     2941 acaagacaaa caaacacccc agcatcatat gtggcccgta accaccgcca atgtccatca
     3001 gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca aacggcatca
     3061 tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcgctt tcagcatcta
     3121 tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt
     3181 catctgtcag cattaacgcc accattagca ccagacagaa aatggcatgg ggtgcctggc
     3241 tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc
     3301 cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg atatcgccca
     3361 gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc tttagcccca
     3421 ccacattcag tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaatcagtt
     3481 ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat
     3541 actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga
     3601 catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat
     3661 taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc
     3721 cataacgctc acgtaatttg agatcatcca gccgttgacc aatcattggc gatccggggc
     3781 gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg
     3841 ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc atgaaacgca
     3901 tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa
     3961 agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc aggttcggcg
     4021 gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc aaaacttaac ggcatcatca
     4081 gacgtgacgg cgacgtttgc atacgcatgg caacgcttaa caccacggga ataaagatag
     4141 cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc atcaacaaaa
     4201 ccaacatttt gatttcacta ttgcccgcaa ctttgaccag ccatgttccc attacggtgg
     4261 caacaccggt acggaccaaa ccatcgccaa taataaacaa ggcggcaatc aggacaacgt
     4321 taggatcaga aaagccggaa aatacttctg ggactgtcag cgttccgctt aatgcaaacg
     4381 cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacga
     4441 tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt
     4501 aattgagggg gatgacttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca
     4561 tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg
     4621 ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg ccggagcgtt
     4681 aaccgcaatg acatgacaac ccgccgccag gccagaaagc acgccagcgg gagcatcttc
     4741 caccaccaca cactcctgcg gcgcaagccc cagcagctgc gcgcctaaca gatacgcatc
     4801 aggttctggt tttccgcgct tcactcgctc agcggttaca aacacctctg gtgcgggaag
     4861 cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca caatggccca
     4921 cggaatacct gctttattca aatgactgag taaggcgatt gcccccggaa gcgcggtaat
     4981 accttcggtt tccgtgacct cgatgtgctc cagacgcgta aactcggcgg caatatcagc
     5041 ctcggatttg cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa
     5101 agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc
     5161 taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg
     5221 cacgcgggcc tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact
     5281 gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct
     5341 gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca
     5401 taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga
     5461 cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat
     5521 gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact
     5581 cgcgatccaa ttcacgcatc tgtaatccgt aaccacgctc aataattgtt tgcaggcgac
     5641 ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa
     5701 taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa
     5761 aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca
     5821 tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg
     5881 taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga
     5941 acttcataaa accagttaag gattgcaggg ggtaatggcg tgacagaacg cttgcccagc
     6001 caccacaatc cctgcatggg taaacttaag gcgaacaggg cagtggcaac tgccggccca
     6061 agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa
     6121 cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt
     6181 ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta
     6241 acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa
     6301 atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca
     6361 ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa
     6421 attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt
     6481 tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat
     6541 catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact
     6601 tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc
     6661 atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg
     6721 cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca
     6781 ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca
     6841 gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat
     6901 accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt
     6961 gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca
     7021 cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa
     7081 gagtcttacc tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac
     7141 ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa
     7201 ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct
     7261 atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc
     7321 atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg
     7381 ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg
     7441 accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg
     7501 aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg
     7561 ctgatggatg gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgca
     7621 atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa
     7681 cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct
     7741 gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact
     7801 gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg
     7861 gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg
     7921 tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc
     7981 tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta
     8041 cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct
     8101 acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa
     8161 actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac
     8221 gtaagcacca gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa
     8281 tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac
     8341 tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca
     8401 aactgaacgc accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg
     8461 acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc
     8521 cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg
     8581 atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg
     8641 ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg
     8701 gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag
     8761 ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggttacgaa atggacgcgc
     8821 gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca
     8881 ccaacacctg gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg
     8941 atcacgaacg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga
     9001 tcgaatctct gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt
     9061 atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg
     9121 aac
//
LOCUS       DACAPB010000177         9094 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.177, whole genome shotgun sequence.
ACCESSION   DACAPB010000177 DACAPB010000000
VERSION     DACAPB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.177"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(108..503)
                     /gene="caiF"
                     /locus_tag="IT904_003827"
     CDS             complement(108..503)
                     /gene="caiF"
                     /locus_tag="IT904_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAP3193541.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(764..3985)
                     /gene="carB"
                     /locus_tag="IT904_003828"
     CDS             complement(764..3985)
                     /gene="carB"
                     /locus_tag="IT904_003828"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP3193542.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(4003..5151)
                     /locus_tag="IT904_003829"
     CDS             complement(4003..5151)
                     /locus_tag="IT904_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HAP3193543.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(5607..6428)
                     /gene="dapB"
                     /locus_tag="IT904_003830"
     CDS             complement(5607..6428)
                     /gene="dapB"
                     /locus_tag="IT904_003830"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP3193544.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(6595..7509)
                     /gene="rihC"
                     /locus_tag="IT904_003831"
     CDS             complement(6595..7509)
                     /gene="rihC"
                     /locus_tag="IT904_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAP3193545.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(7575..8525)
                     /gene="ispH"
                     /locus_tag="IT904_003832"
                     /gene_synonym="lytB"
     CDS             complement(7575..8525)
                     /gene="ispH"
                     /locus_tag="IT904_003832"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAP3193546.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGIYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(8527..8976)
                     /gene="fkpB"
                     /locus_tag="IT904_003833"
     CDS             complement(8527..8976)
                     /gene="fkpB"
                     /locus_tag="IT904_003833"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP3193547.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
BASE COUNT         2132 a         2536 c         2272 g         2154 t
ORIGIN      
        1 gcggcataaa cgccttatcc aacccggctg gagagcaacc gtaagccgga taagatgcgc
       61 cagcatcgca tccggcgacc aggtcgcacg gtatcatcga aatgacatta acgacgcata
      121 ctctttgaca acatcatctg ccacttctgc tcgcggttaa gttccgtgag cggaacggca
      181 ggaatacgcg gtgtctttct tacgccgact aatttttccc gactgttatt gcgcaggcgc
      241 gcgtaaattt gctcatcgat atcgaccact ttaaccagtc gctgacactg gcatccccgc
      301 ccttccagct tattagggat catcttaact tcgcagctta tttctgtgac ttccgacaga
      361 atataagtca gggtattaac cgccttgctg tgttcaatat cgaaatgaat agagatctct
      421 cttgctatca cccaccgatt ttcagccatc atccattcgg cgattaacaa gtagagtggt
      481 ttttcaacat atccttcaca catacgaact ccagtttcac aaattaaaag tctggctatg
      541 actcgttttg aagcaattaa acaaggccat attaaaattg tggatatatc gatagtatgc
      601 aacttcaccg tcgataagtg tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc
      661 ccaaaagatt tacttaatgg gcaaaaaaaa cggtcctcat cagagaaccg tattagttat
      721 tcgatcaaat tagcggatga aaaatatctg ccatgacacg ctattatttg atctgtgcgt
      781 gcatttcctg caccgaaatt actttttcag tcgcatcggc attcagcgcc atcgcggtgg
      841 caaagccgcc gttcagggtg gtgtcgtaat gcactttata ttgcagcgca ctgcgacgaa
      901 tcacgcggga gtcttcaatc gcacgacggc ctgaggtggt gttgatgatg taggtatatt
      961 cgccattctt gatacggtcc tgaatgtgcg gacggccttc atgcaccttg tttaccagac
     1021 gcgggttgat acctgcttcg cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga
     1081 agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttatcg ccttcgcgca
     1141 cggaaagcag cgcacgaccg tgtttcttca tggtggagtt gctgcccagc tgcgctttgg
     1201 caaacgcttc agcgaaggtg cggcccacgc ccatgacttc cccggtagag cgcatttctg
     1261 gccctaacag cgggtcaacg cccgggaatt tattgaacgg cagcaccact tctttcaccg
     1321 agtagtacgg cgggataact tctttggtta cgccctgctc agccagcgat ttgccagcca
     1381 tcacgcgcgc cgccactttt gccagcggta cgccggtggc tttggagacg aacggaacgg
     1441 tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc accgcaaact
     1501 gcacgttcat caggccgcgc acctgcaatt cgaaggccag tttctgcacc tgctggcgca
     1561 tcacatcctg aatttcctga cttaaagtgt aggctggcag cgaacatgcg gagtcaccgg
     1621 agtgcacgcc cgcctgctca atatgctcca tgatgccgcc aatcagcacc atttcgccgt
     1681 cgcagatggc gtccacatcc acttctaccg cgtcatcgag gaagtggtcc agcaacactg
     1741 gcgcatcgtt agacacgctg accgccgtct ggaagtagcg acgcaggtct gcttcgtcat
     1801 agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgtaccacc agcgggtagc
     1861 caatctcttt cgccttctct accgccattt cgatagcggt aacggtggcg ttcgccggtt
     1921 gcttcagttt caggcggtca accgcgtgct ggaagcgttc acggtcttcc gcacggtcga
     1981 tagcatccgg gctggtgccg ataaccggta cgccagcggc ttccagtgca cgcgccagtt
     2041 tcagcggggt ctgaccgccg tactgaacga taacgccttt cggcttctca atacgcacga
     2101 tttccagcac atcttccagg gttaccggct cgaagtagag gcggtcggaa gtgtcgtaat
     2161 cggtggagac ggtttccggg ttacagttaa ccataatggt ttcgtaaccg tcttcgcgca
     2221 gcgccagcga ggcgtgtacg cagcagtagt cgaattcgat accctgaccg atacggttcg
     2281 ggccgccgcc aagcaccatg attttttcac ggtcggtaga cggattcgct tcgcactctt
     2341 cttcataagt ggagtacatg taagcggtgt cggtggcgaa ctctgccgca caggtatcca
     2401 cgcgcttata aaccgggtgc aggtcatact ggtcacgcag cttacggatt tccgcttcgc
     2461 gtacgcccgc cagttttgcc aagcgcgcat cggcaaagcc tttgcgtttc agctggcgca
     2521 ggaagtcagc gttcaggcca gtgatgccca cttccgccac tttctcttcc agacgcacca
     2581 gctcttcaat ctgtaccagg aaccagcggt caatgttggt caggttgaag acgccgtcca
     2641 cagacaggcc cgcacggaac gcatcggcga tgtaccagat acgatctgcg cctgcgtctt
     2701 tcagttcgcg acggattttg gttaacgctt ccgggtcatc caggctcact ttcgggtcga
     2761 atccagtcgc accgacttcc aggccgcgca gcgctttttg cagggattcc tgctgcgtgc
     2821 gaccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg tcgttagcac
     2881 cggcgaattt ttcgaagttg aagcgaggaa ttttagtaac cacatagtcg atggacggct
     2941 cgaaggaggc cggagtacgt ccgccagtga tgtcgttcat cagttcgtcg agggtgtaac
     3001 ccaccgccag tttcgccgcc actttagcaa tcgggaaacc ggtcgctttc gacgccagcg
     3061 ccgaagaacg ggacacgcgt gggttcattt cgataacaat cagacgaccg tttttcgggt
     3121 tcaccgcaaa ctgaacgttg gaaccaccgg tttcaacgcc gatttcacgc agcaccgcca
     3181 tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct ggcgcgacag
     3241 tgatggagtc accggtgtgg atgcccatcg catcgaagtt ttcgatagag cagacgatga
     3301 tgcagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag ccgatcagcg
     3361 actcatcaat cagcaactct ttggtcggag agagatccag accgcgggcg caaatttctt
     3421 caaactcttc acggttataa gcgataccgc cgccgctacc gcccatggta aaggatgggc
     3481 gaataatgca cgggaagccc acgtcagcgg caaccgccag cgcttcttcc atcgtgtgtg
     3541 cgataccgga acgcgcggtt tccagaccaa ttttcttcat cgctacgtcg aaacgacggc
     3601 ggtcttctgc tttatcaatc gcatcggcag tggcaccaat catggtgaca ccgaactctt
     3661 ccaacacgcc ctgacgttcc agctccagcg cgcagttcag cgccgtctga ccgcccatcg
     3721 ttggcagcac cgcgtccggg cgctcttttt caataatctt gcgtacaact tcccagtgaa
     3781 tcggctcgat gtaggttgca tcagccattt ccgggtcggt catgatggtc gccgggttgg
     3841 agttcaccag aatgacgcgg taaccctctt cacgcagggc tttacacgct tgcgcgccag
     3901 agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga atcaggatac
     3961 tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag cggttttacg
     4021 gtactgctca attaactcga taaagtggtc gaacaacggc gcggcgtcgt gtggaccagg
     4081 gctggcttca gggtgcccct ggaagctgaa tgccggttta tcggtgcgat gaatgccctg
     4141 taacgtaccg tcgaacaggg atttatgcgt gacacgcagg tttgcaggta atgttgcttc
     4201 gtccaccgca aaaccgtggt tctgggcggt gatcattacc acgtttttct ccacatcttt
     4261 aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg caccgctcgc
     4321 cagcgccagc agctgatgac cgagacagat gccgaatacc ggaatatcgg tttcgaggaa
     4381 tttctggatg gcggtaatgg cgtaatcgca cggggccggg tcgccaggac cgttggagag
     4441 gaagatgccg tctggattca ttttcagcac atcttccgca gaagtttgcg ccggaacgat
     4501 ggtcaggcga cagcctctat ccaccagcat ccgcaggatg ttgcgcttgg caccaaaatc
     4561 ataagccacg acgtggaacg gcagctcgtc ttcttttttc gcttctggca ggccaccggt
     4621 caacgtccag ctcccttgtg tccagctata ggcttctgcg gtggtcactt cttttgccag
     4681 atccatgcca ttcagacctg ggaacgcgcg ggctttttct aacgccagcg ccgcatccgg
     4741 gttatcgccc gcgataatgc agccattctg tgcgcctttc tcgcgcagta aacgcgtcag
     4801 cttacgggta tcgatatcgg caatcgccac gatgttatgg cgtttcaggt aagaagagag
     4861 gtcttcggta ttacggaagt tgctggcaat cagcggcagg tcgcgaatca ccagaccttg
     4921 tgcatgtacc tgagaagatt cttcatcggc gtcattggtg ccgacattgc caatatgggg
     4981 ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt cttgataacc
     5041 ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa cctgttgccc ctatggcccg
     5101 accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca aaacaccctc
     5161 cagagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt aaatcaatgc
     5221 aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat gggcgctaaa
     5281 agtcaactta atggcgggat tttttatatt attttgtggc tcatgcatgt tttacaggca
     5341 ttaagcgaca aaaagatcat ctaacctgtt ctggaaaacg cttgcgcagc aagaagattg
     5401 catgaaataa aaacgagaag aaaaaagtgg accaaatggt caaaatttac attaaaacaa
     5461 caaaaacaga agacaaaaca tgtaattttt ggcacttata gacaatttaa aaacataaca
     5521 ccaaaaataa aagggccaaa aattaaagcc ctttataatc aataaattaa atgtgttatt
     5581 tttgcaccat aacaaatatt ttgtggttac aaattattga gatcaagtac atctcgcata
     5641 tcaaaaagac cgctttcctt accactcaac cacaaagccg atcttaccgc gccgttagca
     5701 aatgtcatac ggctggacgc cttatgggtg atctccagac gctcgccaat atcggcaaac
     5761 atcgcggtat gttcaccaac gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca
     5821 ggcacacgtt caccggtgtg gccttcacga ctgtagaccg cgcaatcttt cagatcttta
     5881 tcaagggcgt gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact
     5941 ttatgtctat gatgtgcttc aataatttcg atatcggtgt agtcacccat cactttggct
     6001 gctttctcca gcagcttaag catgacgtta acgccaacgc taaaattggc agcaaagaca
     6061 atcgcaatat cggcagcggc gtcacgaatt gcttgtttac cggcttcgtc aaaccccgta
     6121 gtgccgatca ccatcccttt gccatgctgg cgacaaaaag cgagatggtt cagcgtacct
     6181 tccggacggg taaaatcgat aaacacatca aaatcatctt ttaccgcatc gaggctgctt
     6241 tgcacggtaa cgcctgtttt cccggctccg gccagctcac cggcgtcgct gcccagtaaa
     6301 gaagatcctt cacgctccag cgcagcgccc aactgcacgc cctctaatgc cagcgccgcc
     6361 tgaatcaact ggcggcccat acgccccccg gctcccgcga tggcaacgcg gatgtttgca
     6421 tcatgcatag ctattctctt ttgttaattt gcatagaccg ttttcagagt aaccagcgca
     6481 tcgctacgcc gccagccaaa aacgctgata attagaaatt aatgataaac agggagggat
     6541 atcagtgaaa ggcatgagtc gatgaatgac tgcatgccaa taacatgtga caggttacga
     6601 cgccagagcc agcacctcag ccacccactg ctggaagcct ttcacatcca gatccaatgc
     6661 cacctgtaca ttggctggct tgcccaggca accgtcgata tcaaccaccg tcgtgcctga
     6721 ggtaaattcg ccctgagttt ccactgccac aaaacagggt ttgagagtga acaggtccgg
     6781 gcgcaccagc caggcgatgg cgcagagatc gtgcattcgc aagccgcttt gcatactgcc
     6841 gctacggtag tggctaaaca gggcgtgaag cattttcccg gtacggttta actgcggcag
     6901 tgtagagaga tagtcaggag ttaatattgc ctgattggtg acatccaaac cgcacatgac
     6961 gatttcaata ccactgcgga agacacaggc agcagcttct ggatcggcag caatattaaa
     7021 ctcggcgttt ggcgtacagt tgccgcgtcc ggcagaacca cccatgatca ccagacggcg
     7081 aatatacggc ttgcattccg ggcattgtga aagtaacagc gcaatattgg ttaacgggcc
     7141 gatggccacc agggtaacag gctctggtgc acgcatcagg gcatcccgaa tcgccagaaa
     7201 cgccggtatc ccgagcggct ttcggttgtg ctcaacaaag tcgtagccag ccattcccga
     7261 ttcgccgtgc acagatgccg catcacgcgg tgcgcgtacc agtggcacag cggccccttg
     7321 ggcgagcgga atctccgcat tccagaaatg cagcagttgc agggcattgc gggtagtttt
     7381 ctcaaccgag acattacccg cgacggtggt catcagttgc aggtcgagtt cgggtgcaaa
     7441 aatcgcggcg gcaatggcga cggcatcgtc aatgccgggg tcagtatcga ggaagatagg
     7501 taaacgcatg ttttctccat aaaaaatgcc ggtaacaaga ccggcatttt cgcataactt
     7561 aggctgctaa tgacttaatc gacttcacga atatcgacac gcagctcttt cggcacttcg
     7621 aaaacaatgt tttcttcacg gccttccagc ggaatggctt caccaccgcc cagctgctgc
     7681 aaacgtgcca ccacattctg caccagaata tccggagccg atgcgcccgc agtcacgccg
     7741 acgcatttaa cctctttcac ccactcttcc tggatgtctt tcgcatcgtc aatcaaaaac
     7801 gcgcgtttgc ccatacgctg ggccagctcc gccagacggt tggagttgga ggagtttttc
     7861 gaaccgacca ccaacacaac ttccgcctgt tctgccaggg cgcgtaccgc ttcctgacgg
     7921 ttagtcgtgg cgtagcagat gtcatctttg cgcggaccga caattttcgg gaagcgttta
     7981 cgcagcgcgt cgatcacatc agacgtgtca tccaccgaca gcgtggtctg ggtcataaag
     8041 gagagcttct cttcgttttt gaccgtcagt ttccacacat cgtccggcga ttcgaccaga
     8101 tatattcccc cttccgggtt actgtactgg cccattgtcc cttccacttc cgggtgcccg
     8161 gcgtgaccga tgagaataga ttcttcgcca cggcgactgg cgcgggcgac ttccatatgc
     8221 actttggtca ccagcggaca ggtggcatca aacaccgtca aatcgcgact ttttgcttcg
     8281 ttacgtaccg cctgagaaac accgtgtgcg gagaaaatca ggatcgcgcc gtccggtact
     8341 tcgctaatct gctcaataaa gatagcccca cgctcacgca agctatcgac cacatagcgg
     8401 ttatgtacca cttcgtgacg gacatatatc ggtgcgccgt aaatggccag cgcgttttca
     8461 acaatgctga tagcgcggtc taccccggca caaaaaccac gcgggttggc caacaggatc
     8521 tgcatgttac gcctccagtg ccggatcgat ttccagcact tcaatatcaa aatgaacggt
     8581 ctgcccggcc agcggatggt tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat
     8641 cacgccaggc atctcactgc catccattgc ggtaaaaagc atgattgcgc caatttctgg
     8701 ctcgcctgca tccataaatt cacggcggga gaagtactga atcaggtccg gtgacggcac
     8761 gccaaacgcc gcatctggct ccaacgagaa ggtggtttta tcgcccactt tcagccccaa
     8821 caggtgttgc tccagccctt cagaaagaga agcatcaccc aggcggaaca gcgccggttt
     8881 accgttgttg cgggtagact cggcggtggt gccatcgtcg agttttagcg tgaagtgcac
     8941 caggacggcg ctattgctct gtacagattc agacatgcag gtttgctctt atatttaagg
     9001 atttttgccg gatgcggcgt aaacgcctta tccggcctac gatttcgcag caatctgtag
     9061 gccggataag atgcgtcagc atcgcatccg gcag
//
LOCUS       DACAPB010000178         9001 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.178, whole genome shotgun sequence.
ACCESSION   DACAPB010000178 DACAPB010000000
VERSION     DACAPB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.178"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..272)
                     /locus_tag="IT904_003834"
     CDS             complement(<1..272)
                     /locus_tag="IT904_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193548.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSN"
     gene            complement(395..1477)
                     /locus_tag="IT904_003835"
     CDS             complement(395..1477)
                     /locus_tag="IT904_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP3193549.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(1554..2501)
                     /gene="mhpR"
                     /locus_tag="IT904_003836"
     CDS             complement(1554..2501)
                     /gene="mhpR"
                     /locus_tag="IT904_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HAP3193550.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHHVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQDRKELIEMLASRPGDDYQLAR
                     EPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGMHLR"
     gene            2578..4242
                     /locus_tag="IT904_003837"
     CDS             2578..4242
                     /locus_tag="IT904_003837"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HAP3193551.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            4244..5188
                     /locus_tag="IT904_003838"
     CDS             4244..5188
                     /locus_tag="IT904_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HAP3193552.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            5206..6072
                     /locus_tag="IT904_003839"
     CDS             5206..6072
                     /locus_tag="IT904_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxononadienedioate/2-hydroxy-6-
                     oxononatrienedioate hydrolase"
                     /protein_id="HAP3193553.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSVVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            6082..6891
                     /gene="mhpD"
                     /locus_tag="IT904_003840"
     CDS             6082..6891
                     /gene="mhpD"
                     /locus_tag="IT904_003840"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HAP3193554.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            6888..7838
                     /locus_tag="IT904_003841"
     CDS             6888..7838
                     /locus_tag="IT904_003841"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAP3193555.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            7835..8848
                     /gene="dmpG"
                     /locus_tag="IT904_003842"
     CDS             7835..8848
                     /gene="dmpG"
                     /locus_tag="IT904_003842"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HAP3193556.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
BASE COUNT         1953 a         2376 c         2636 g         2036 t
ORIGIN      
        1 cagtttgagg ggacgacgac agtatcggcc tcaggaagat cgcactccag ccagctttcc
       61 ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag gctgcgcaac
      121 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      181 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      241 acgacggcca gtgaatccgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      301 gctcacaatt tcacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      361 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      421 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      481 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
      541 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
      601 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
      661 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
      721 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
      781 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
      841 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
      901 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
      961 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1021 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1081 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     1141 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     1201 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgag ggcgcgtgca
     1261 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     1321 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     1381 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     1441 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     1501 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtcaacgt
     1561 aaatgcatgc cgcttcgcct tccggccacc agaatagcct gcgattcaac cccttcttcg
     1621 atctgttttg ctacccgttg tagcgccgga agatgctttt ccgctgcctg ttcaatggtc
     1681 attgcgctcg ccatatacac cagattcaga cagccaatca cccgttgttc actgcgcagc
     1741 ggtacggcga tagaggcgat cttctcctcc tgatcccagc cgcggtagtt ctgtccgtaa
     1801 ccctctttgc gcgcgcgcgc cagaatggct tccagcttta acggttcccg tgccagttga
     1861 tagtcatcac cggggcggga ggctaacatt tcgattaatt ccttgcggtc ttgttccggg
     1921 caaaaggcca gccaggtcag gcccgaggcg gttttcagaa gcggcaaacg tcgcccgacc
     1981 attgcccggt gaaaggataa gcggctgaaa cggtgagtgg tttcgcgtac caccattgca
     2041 tcaacatcca gcgtggacac atctgtcggc cataccactt cgcgcaacag atcgcccagc
     2101 agtggggccg ccagtgcaga aatccactgt tcgtcacgaa atccttcgct taattgccgc
     2161 actttgatgg tcagtcgaaa actatcatcg gaggggctac ggcggacata tccctcttcc
     2221 tgcagcgtct ccagcagtcg ccgcacagtg gtgcgatgca ggccgctgag ttccgccagc
     2281 agcccgacgc tggcaccgcc atcaagttta tttaacatat ttaataacat tagaccgcgg
     2341 gttaagccgc gcacggtttt gtattccgtc tgctcattgt tctgcatatt aattgacatt
     2401 tctatagtta aaacaacgtg gtgcacctgg tgcacattcg ggcatgtttt gattgtagcc
     2461 gaaaacaccc ttcctatact gagcgcacaa taaaaaatca tttacatgtt tttaacaaaa
     2521 taagttgcgc tgtactgtgc gcgcaacgac attttgtccg agtcgtgagg tactgaaatg
     2581 gcaatacaac accctgacat ccagcctgct gttaaccata gcgttcaggt ggcgatcgct
     2641 ggtgccggcc cggttgggct gatgatggcg aactatctcg gccagatggg cattgacgtg
     2701 ctggtggtgg agaaactcga taagttgatc gactacccgc gtgcgattgg tattgatgac
     2761 gaggcgctgc gcaccatgca gtcggtcggc ctggtcgatg atgttctgcc gcacactacg
     2821 ccgtggcacg cgatgcgttt tctcaccccg aaaggccgct gttttgctga tattcagcca
     2881 atgaccgatg aatttggctg gccgcgccgt aacgccttta ttcagccgca ggtcgatgcg
     2941 gtgatgctgg aaggggtgtc gcgttttccg aatgtgcgct gcttgttttc ccgcgagctg
     3001 gaggccttca gtcagcaaga tgacgaagtg accttgcacc tgaaaacggc agaagggcag
     3061 cgggaaatag tcaaagccca gtggctggta gcctgtgacg gtggagcaag ttttgtccgt
     3121 cgcactctga atgtgccgtt tgaaggtaaa actgcgccaa atcagtggat tgtggtagat
     3181 atcgccaacg atccgttaag tacgccgcat atctatttgt gttgcgatcc ggtgcgcccg
     3241 tatgtttctg ccgcgctgcc tcatgcggta cgtcgctttg aatttatggt gatgccggga
     3301 gaaaccgaag agcagctgcg tgagccgcaa aatatgcgca agctgttaag caaagtgctg
     3361 cctaatccgg acaatgttga attgattcgc cagcgtgtct acacccacaa cgcgcgactg
     3421 gcgcaacgtt tccgtattga tcgcgtactg ctggcgggcg atgccgcgca catcatgccg
     3481 gtatggcagg ggcagggcta taacagtggt atgcgcgacg cctttaacct cgcatggaaa
     3541 ctggcgttgg ttatccaggg gaaagcccgc gatgcgctgc tcgataccta tcaacaagaa
     3601 cgtcgcgatc acgccaaagc gatgattgac ctgtccgtga cggcgggcaa cgtgctggct
     3661 ccgccgaaac gctggcaggg tacgttacgt gacggcgttt cctggctgtt gaattatctg
     3721 ccgccagtaa aacgctactt cctcgaaatg cgcttcaagc cgatgccgca atattacggc
     3781 ggtgcgctga tgcgtgaggg cgaagcgaag cactctccgg tcggcaagat gtttattcag
     3841 ccgaaagtca cgctggaaaa cggcgacgtg acgctgctcg ataacgcgat cggcgcgaac
     3901 ttcgcggtaa ttggctgggg atgcaatcca ctgtggggga tgagcgacga gcaaatccag
     3961 cagtggcgcg cgttgggcac acgcttcatt caggtggtgc cggaagtgca aattcatacc
     4021 gcacaggata accacgacgg cgtactacgc gtgggcgata cgcaaggtcg cctgcgtagc
     4081 tggttcgcgc aacacaatgc ttcgctggtg gtgatgcgcc cggatcgctt tgttgccgcc
     4141 accgccattc cgcaaaccct gggcaagacc ctgaataaac tggcgtcggt gatgacgctg
     4201 acccgccctg atgccgacgt ttctgtcgaa aaggtagcct gatatgcacg cttatcttca
     4261 ctgtctttcc cactcgccgc tggtggggta tgtcgacccg gcgcaagagg tgctcgatga
     4321 ggtcaatggc gtgattgcca gcgcccgcga gcgtattgcg gcattctccc ctgaactggt
     4381 ggtgctgttt gcgccagatc actacaacgg ctttttctat gacgtgatgc caccgttctg
     4441 tttaggcgtt ggagcgacgg caattggtga tttcggcagt gcggcaggag agctgcccgt
     4501 gcctgtggag ctggcggagg cctgtgcgca tgccgtcatg aagagcggga tcgatcttgc
     4561 cgtttcttac tgtatgcagg tggaccacgg gttcgcccag ccgctggagt tcctgctcgg
     4621 tgggctggat aaggtgccag ttctgcctgt gttcatcaac ggtgtcgcca cgccgctgcc
     4681 cggtttccag cgtacccgca tgttgggtga agccattgga cgtttcacca gcactctcaa
     4741 taaacgcgtg ctgttcctgg gttccggtgg gctttcccat cagccgccgg tgcccgaact
     4801 ggcgaaagcc gatgcccata tgcgcgaccg tctgttgggg agcgggaaag atttacccgc
     4861 cagtgagcgc gaattgcgtc agcaacgggt gattagcgcc gctgagaagt ttgttgagga
     4921 tcagagaacg ctgcatccgc tcaacccgat ttgggataac cagttcatga ctttgctgga
     4981 gcagggacgc atacaggaac tggatgccgt cagtaacgaa gagctttccg ccattgccgg
     5041 aaagtcgaca catgaaatca aaacctgggt cgccgctttt gccgctattt ctgcgtttgg
     5101 caactggcgt agcgaagggc gttattaccg cccaatcccg gagtggattg ccggatttgg
     5161 ctcgttaagc gccagaacag agaactgaat atgcaggaga agatgatgag ttatcagcca
     5221 caaaccgaag ccgccaccag ccgttttctg aatgtagaag aagcgggtaa aacgctgcgc
     5281 atccatttta atgactgcgg acaaggcgac gaaaccgttg tcctgctgca tggttccggc
     5341 ccgggtgcta ctggctgggc gaacttcagc cgcaatatcg atccgctggt agaggcgggc
     5401 tatcgggtga tcctgctgga ttgtccgggt tggggcaaga gcgattcggt cgttaatagt
     5461 ggttcgcgat cggatcttaa tgcacgaatc ctgaaaagcg tggtggatca actggatatc
     5521 gccaaaatcc acctgctggg caactcgatg gggggccata gttctgtggc gttcaccctt
     5581 aaatggccgg agcgcgtcgg caaactggtg ctgatgggcg gcggtacggg cggcatgagt
     5641 ttgtttacgc cgatgccaac cgaaggtatt aagcgactga atcagcttta tcgtcagccg
     5701 actatcgaaa acctgaagct gatgatggat atcttcgttt ttgataccag cgatttgacc
     5761 gacgccctgt ttgaagcgcg cctgaataat atgctgtcgc gccgcgatca cctggaaaac
     5821 ttcgttaaga gcctggaagc taatccgaaa cagttcccgg attttggccc acgtctggcg
     5881 gaaatcaaag cgcaaaccct gattgtctgg gggcgcaacg accgctttgt gccgatggat
     5941 gcgggtctgc gtctgctgtc cggcattgcc ggttctgaac tgcatatctt ccgcgactgt
     6001 ggtcactggg cgcagtggga acatgccgac gctttcaatc aattggtgct gaatttcctc
     6061 gcacgccctt aaggaatggt catgacgaag catactcttg agcaactggc ggcggattta
     6121 cgccgcgccg cagagcaggg cgaagcgatt gcgccgctgc gcgatctgat tggtatcgat
     6181 aacgctgaag cggcctacgc cattcagcac ataaatgtgc aatatgacgt tgtgcagggg
     6241 cgtcgcgtgg tagggcgtaa agtgggcctg acgcatccga aagtgcaaca acaactgggc
     6301 gttgatcaac cggattttgg gacgttattt gccgacatgt gttatggcga taacgaaatc
     6361 attccttttt cccgtgttct gcaaccccgc attgaagcgg agatcgcact ggtgttgaac
     6421 cgcgatttac ccgcaactga tatcaccttc gacgaactgt ataacgccat tgaatgggta
     6481 cttccggcgc tggaagtggt ggggagccgc attcgcgact ggtcgattca gtttgtcgat
     6541 accgtggcag ataacgcttc ctgcggggtg tatgtcatcg gcggtccggc gcaacgtccg
     6601 gcggggttag acctgaaaaa ctgcgccatg aagatgacgc gtaataacga agaggtttcc
     6661 agcgggcgcg gcagcgaatg cctgggacat ccgcttaatg ccgccgtctg gctggcacgc
     6721 aaaatggcca gtctgggtga accgctgcgc gccggagata tcattcttac cggggcatta
     6781 ggcccgatgg tggcggtgaa tgcgggcgat cgttttgaag cccatattga aggcataggt
     6841 tcagttgctg cgacattttc aagcgcagcc ccaaaaggaa gtctgtcatg agtaagcgta
     6901 aagtcgccat tatcggttct ggcaacattg gcaccgatct gatgattaaa attttgcgtc
     6961 acggtcagca tctggagatg gcggtgatgg tcggcattga tcctcagtcc gacggtctgg
     7021 cgcgtgccag acgtatgggc gtcgccacca cccatgaagg ggtgatcggg ctgatgaaca
     7081 tgcctgaatt tgctgatatc gacattgtat ttgatgcgac cagcgcgggc gcgcatgtga
     7141 aaaacgatgc cgctttacgc gaagcgaaac cggatattcg cttaattgac ctgacgcctg
     7201 ctgccatcgg cccttactgc gtgccggtgg ttaacctcga ggcgaacgtc gatcaactga
     7261 acgtcaacat ggtcacctgc ggcggccagg ccaccattcc aatggtggcg gcagtttcac
     7321 gcgtggcgcg tgttcattac gccgaaatta tcgcttctat cgccagtaaa tctgccggac
     7381 ctggcacgcg tgccaatatc gatgaattta cggaaaccac ttcccgagcc attgaagtgg
     7441 tgggcggcgc ggcaaaaggg aaggcgatta ttgtgcttaa cccagcagag ccaccgttga
     7501 tgatgcgtga cacggtgtat gtattgagcg acgaagcttc acaagatgat atcgaagcct
     7561 caatcaatga aatggctgag gcggtgcagg cttacgtacc gggttatcgc ctgaaacagc
     7621 gcgtgcagtt tgaagttatc ccgcaggata aaccggtcaa tttaccgggc gtggggcaat
     7681 tctccggact gaaaacagcg gtctggctgg aagtcgaagg cgcagcgcat tatctgcctg
     7741 cctatgcggg caacctcgat attatgactt ccagtgcgct ggcgacagcg gaaaaaatgg
     7801 cccagtcact ggcgcgcaag gcaggagaag cggcatgaac ggtaaaaaac tttatatctc
     7861 ggacgtcaca ttgcgtgacg gtatgcacgc cattcgtcat cagtattcgc tggaaaacgt
     7921 tcgccagatt gccaaagcac tggacgatgc ccgcgtggat tcgattgaag tggcccacgg
     7981 cgacggtttg caaggttcca gctttaacta tggtttcggc gcacatagcg accttgaatg
     8041 gattgaagca gcggcggatg tggtgaagca cgccaaaatc gcgacgttgt tgctgccagg
     8101 aatcggcact attcacgatc tgaaaaatgc ctggcaggct ggcgcgcggg tggttcgtgt
     8161 ggcaacgcac tgtaccgaag ctgatgtttc cgcccagcat attcagtatg cccgcgagct
     8221 cggaatggac accgttggtt ttctgatgat gagccatatg accacgccgg agaatctcgc
     8281 caagcaggca aagctgatgg aaggctacgg tgcgacctgt atttatgtgg tggattctgg
     8341 cggtgcgatg aacatgagcg atatccgtga ccgtttccgc gccctgaaag cagagctgaa
     8401 accagaaacg caaactggca tgcacgctca ccataacctg agtcttggcg tggcgaactc
     8461 tatcgcggcg gtggaagagg gctgcgaccg aatcgacgcc agcctcgcgg gaatgggcgc
     8521 gggcgcaggt aacgcaccgc tggaagtgtt tattgccgcc gcggataaac tgggctggca
     8581 gcatgggacc gatctctatg cgttaatgga tgccgccgac gacctggtgc gtccgttgca
     8641 ggatcgaccg gtacgagtcg atcgcgaaac gctggcgctg ggatacgctg gtgtttactc
     8701 gagcttcctg cgtcactgtg aaacggcggc ggcgcgttat ggcttaagtg cggtggatat
     8761 tctcgttgag ctgggcaaac gccggatggt tggcggccag gaggatatga tcgtcgacgt
     8821 ggcgctggat ctgcgcaaca acaaataatg atgactgccg ataacgtgca ttttgtatgc
     8881 ccatcccagc acgactgccg gatgcggcgt aaacgtcttg tccggcctac gggtggcgtg
     8941 agaatttgta ggcctgataa gacgtgttag cgtcgcatca ggcatctgcg cacgacagcc
     9001 g
//
LOCUS       DACAPB010000179         8977 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.179, whole genome shotgun sequence.
ACCESSION   DACAPB010000179 DACAPB010000000
VERSION     DACAPB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.179"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..665
                     /locus_tag="IT904_003843"
     CDS             <1..665
                     /locus_tag="IT904_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAP3193557.1"
                     /translation="AIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRI
                     LWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDY
                     GEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFI
                     MIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDV
                     KA"
     gene            complement(804..1433)
                     /locus_tag="IT904_003844"
     CDS             complement(804..1433)
                     /locus_tag="IT904_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAP3193558.1"
                     /translation="MTRTLKPLILNTSALTLTLILIYTGISAHDKLTWLMEVTPVIIV
                     VQLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1556..3202)
                     /locus_tag="IT904_003845"
     CDS             complement(1556..3202)
                     /locus_tag="IT904_003845"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAP3193559.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEETLSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(3280..4620)
                     /locus_tag="IT904_003846"
     CDS             complement(3280..4620)
                     /locus_tag="IT904_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP3193560.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(5191..5910)
                     /gene="dcuR"
                     /locus_tag="IT904_003847"
     CDS             complement(5191..5910)
                     /gene="dcuR"
                     /locus_tag="IT904_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAP3193561.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5907..7538)
                     /gene="dcuS"
                     /locus_tag="IT904_003848"
     CDS             complement(5907..7538)
                     /gene="dcuS"
                     /locus_tag="IT904_003848"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAP3193562.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7719..7949
                     /gene="yjdI"
                     /locus_tag="IT904_003849"
     CDS             7719..7949
                     /gene="yjdI"
                     /locus_tag="IT904_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAP3193563.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNVKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7961..8233
                     /locus_tag="IT904_003850"
     CDS             7961..8233
                     /locus_tag="IT904_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP3193564.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8460..8756
                     /gene="ghoS"
                     /locus_tag="IT904_003851"
     CDS             8460..8756
                     /gene="ghoS"
                     /locus_tag="IT904_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP3193565.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            8784..8957
                     /gene="ghoT"
                     /locus_tag="IT904_003852"
     CDS             8784..8957
                     /gene="ghoT"
                     /locus_tag="IT904_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HAP3193566.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
BASE COUNT         2228 a         2116 c         2269 g         2364 t
ORIGIN      
        1 ttgctatcta ttatttctca tatgttatcg gtgatgcgga tttgttcccc tattatctgt
       61 cgtatgcggg agctgctaac ctggtgacgt tagtattctt cccacgctta gttaaatcat
      121 tatcccgacg cattttatgg gccggagcat ctattcttcc ggtgttaagc tgtggtgttc
      181 tcctgttaat ggcattaatg agctatcaca acgtcgtcct cattgtgatt gcgggtattt
      241 tgctgaatgt gggaacggcg cttttctggg tattacaggt catcatggtg gcagataccg
      301 ttgattacgg tgaatataaa ctgcacgtac gctgtgaaag tatcgcttac tccgtgcaga
      361 ctatggtagt gaagggcggt tcagcctttg cggctttttt cattgcggtt gtgttaggga
      421 tgattggcta tgtaccgaat gttgaacagt ctacgcaagc cctattaggt atgcagttta
      481 ttatgattgc tctaccaact ctgtttttca tggtaacgct gattctctac ttccgtttct
      541 atcgcctcaa tggtgacacg ctgcgcagga tccagatcca tctgctggat aaatatcgca
      601 aagtaccgcc cgagcctgtt catgctgata ttccggtcgg tgcagtgagt gatgtgaaag
      661 cctgacgtgg acaatagcta acgaaaatgg cctgatgcga tatgtttatc aggccacgtc
      721 ttaatgtaat gctttggatt tgcaaatttt gtagaccgga taaggaattc acgccgcatc
      781 cggcatcaac aaagcgcaag ttgttatccg gttatcaagc caaagcgccg tagctggcgg
      841 caatgaaagc gagcgaggaa tatcaccgtc gttaatgcgc caagcagcgc gcagaacata
      901 tcggattgcg tgtcccactg gtcgccctgg gtgccgagaa aatcatccgc tccctgaccc
      961 attgccagcg ctgcccacca ctcgattaat tcatacatcg cgcttatcgc cagcgccacg
     1021 cagcagacca ggaacgccac cattttacgt ccgcgcacgt acatcccgcg aacgagaatt
     1081 tctcgtgcca ccagtgcagg caccagcccc tggaaaaagt gccccagctt gtcatacgga
     1141 ttacgactca accccagcca ttcctgcacc tcaaaaccaa cggggacttt cgcgtaggtg
     1201 tattgtccgc cgaccatcag gatgatggcg tgaaggaaaa tgagcgtata gagcagcggc
     1261 gttaacggat aacgtctggc ggtggcaagc agtagctgca cgacaataat caccggtgtc
     1321 acttccatca gccaggtgag tttgtcatgg gccgaaatgc cggtataaat caggattaac
     1381 gttagcgtca gtgcgctggt gttaagaatt aacggcttga gtgtgcgggt catggtgagt
     1441 tcgcaaatca gggaaaatac tgactattca ccagcaagcg tgaaattaca atcgcaaaag
     1501 aggtggccca gggggatcac ctggcagcat gctgccaggc gctgggccga agaggttact
     1561 tagtgcagtt cgcgcactgt ttgttgacga tttgctggaa gaagtcgtta cctttgtcat
     1621 cgaccaggat aaacgccggg aaatcttcta cttcgatttt ccagatagct tccataccca
     1681 gctccggata agcgacgcac tccagatgct tgatgctctg ctgcgccagt accgccgccg
     1741 gaccgccgat gctaccgagg tagaagccgc cgtgtttatg acacgcgtcg gtaacctgct
     1801 gactgcggtt acctttcgcc agcatgatca tgctgccgcc gtgggattgc agcagatcca
     1861 cgtaggagtc catacggcct gcggtggttg ggccaagtga acctgatgga taaccggcag
     1921 gggttttcgc cggacccgcg tagtagatcg ggtgatcttt gatgtactgc ggaagttctt
     1981 taccggcgtc aatcagctct ttcagcttgg cgtgtgcaat atctcggccc acgataatgg
     2041 tgccggtgag cgacaaacga gtggataccg ggtattgcga aagctgggcg aggatctctt
     2101 tcatcgggcg gttaaggtca actttcaccg cttcgccttc accggcctgg cgcagttctt
     2161 gtggaatgta ctggcctggg ttgtgttcca gtttttcgat ccagatacct tcgcggttga
     2221 ttttcgcttt aatgttacgg tcagcggagc aggagacgcc catgccgacc gggcaggatg
     2281 cgccgtgacg tggcagacgg ataacgcgaa tgtcgtgcgc gaagtattta ccgccaaact
     2341 gcgcgccaag accgagtttc tgggcctctt ccagcagttc ctgttccagc tggacatcgc
     2401 ggaacgcctg accatgttcg ttcccttccg tcggcagttc atcgtaatag tgagcgcttg
     2461 ctaacttgac ggttttcagg ttggtttccg cagacgtacc gccaatcaca aacgcgatat
     2521 ggtacggcgg gcaggctgca gtaccgaggg tacgcatttt ctcgacgagg aagtttttca
     2581 gtttgccggg agtcagcagg gctttggttt cctggtagag atacgttttg ttggcagagc
     2641 cgccgccttt cgcaacgcaa aggaatttgt actcatcgcc atctaccgcg tacaggtcga
     2701 tttgcgcagg caggttagtg ccggtgttga cctctttgta catgtccagc gccgcattct
     2761 gtgaatagcg caggttatct tcgatatagg tgttatagac gcctttcgac agcgtttctt
     2821 catcaccgcc gccggtccac acgcgctggc cttttttacc gacgatgatc gcggtgccgg
     2881 tatcctggca ggtcggcagc acgcctttgg cggcgatttc ggagtttctt aagaattgca
     2941 gcgccacgta cttgtcgttt tcgctggctt ctggatcgtg aagaatagcc gcaacctgtt
     3001 tctggtgtgc cgggcggagc ataaaagaag cgtcgtgaaa ggcttgctgc gccagcaggg
     3061 tcagggcttc tggttccact ttcaggatgg tttcgccgtc gaagtcggca acgctaacgt
     3121 aatcggaagt gagtagatag tattcggtat tgtctttccc catcgggaaa ggtgcctggt
     3181 agataaaggg tttgtttgac atagcttcca gcctgtaact ctgtatttgt tattcgaaaa
     3241 tggcgtgccg cgtagtcacg ggcggcacgc aaagtgcatt tataagaacc cgtacatcgc
     3301 ggcgaagatc cagccgaaga cgcacgatac gctcacacca atcaaccccg gcagaataaa
     3361 gctgtggttg atgacgaagc gaccgatgtg ggtggtgccg gaacggtcaa actgaatcgc
     3421 tgccagatcg ctcggataag tcggcaggat gtaataaccg tagcaagccg gtgctgaagc
     3481 cacgatgtat gccggatcaa cgccgatcgc cagcgcgacc ggaacaatcg ccgccagcgc
     3541 cgcagcctga gagtttacaa acttggaaac cagcagcaga acaatggcat aggcccacgg
     3601 atactctttc accatttcac ccagtacgcc ctgaatttca gacatatgcg caccgaacat
     3661 ggtttctgcc atccatgcga taccgtacac cgccacgatg gcgatcatac cggaacggaa
     3721 gacttcgttt tttgagatag acgcgggatt ggttttggtc aggataataa tcagcgcccc
     3781 ggtcagcagc ataaacatct gaataaccag taccatcgac agcggtttgc cgccgaagga
     3841 tggacgcagg tccgaatcag caccaagaag ggcgactaca gcgattgccc cgaggaaaat
     3901 ccacattgcc agccagttgc ttttcggcag ttttttatcc agcagcgtcg cggtatcacc
     3961 gtaaacatac tcacggtttt ccggtacgga gatgaatttc tggaactctt cgtctttatc
     4021 cagatcttta ccgcggaacc agctgaagat accgatcgcc aggataccga ttaacgtcga
     4081 tggaatggtg attgccagca gatcgaggaa ctcaagatgg cgaccatcaa aggtgacatt
     4141 acccagcatc gcaaccagag acacgaccgc aaccgacacc ggactggcga taatccccat
     4201 ctgtgcaccg atagaacttg ccgccatcgg acgttccgga cggatgttgt tcttaatggc
     4261 gacgtcgtag atgatcggca gaatggtgta aaccacatga cccgtaccgc aaagaatggt
     4321 cagtgtacag gtcacaaacg gcgcgacaat tgagacatat ttcgggttgc ggcgcagcag
     4381 cttctcggca atttgcagca tgacatcaag accgcccgaa gcttgcaagg tcgccgatgc
     4441 cgccaccacc gcaatgataa ccagcatgac atcaactggt ggtttacctg gctgaaggtg
     4501 gaagacgaag accagaatga ccagaccgat accgcctaat aaacccagcg cgataccacc
     4561 ctttctggca ccataaaaca gacatatcag tattatgata agttggatag taaataacat
     4621 gtgtgaaccc tcgcgataat cctatttaaa tttttgctga atagatcaca gtcacgttct
     4681 gttttgtatg aactgttttc agagcctgac taaatatccg tttggtcgtt accggcttta
     4741 gcaaatacct cacagtgaat attggctgat taaagcagcg gcaaatttat gcactgtgat
     4801 aagcgacttt ttcagagaga aagactctct ggcgatgggt ttatagtttg tactgcgcag
     4861 atgttaatgt tttgtttgct gttatcttat tgatatttat cggtctattt tttgttctca
     4921 gggcagatgt cattaggttt atagattaat ctgatctacc catttgtggg taaaaataca
     4981 cataatgcgg gtgacataat agttaattaa cttttgttag cgttttgaaa ttaaaaacac
     5041 cgttcacctg aagagatatt aatttttagc gatgatggag ggataattat atttgatctg
     5101 gcacaagttt tactgatgaa ggatgtaact tgtgccaggg gtattttgca ttacggtaat
     5161 aattattact tacagataac cagcgatccg ttattggcaa tattgtttca gtagtgagta
     5221 gtgttctgcc tgaatacggt aacggtaaac tggacgcccc gtgacgccat aatggatact
     5281 ggtgaacaag atgtggcagt tgaccagcca gatgaggtat ttacggcagg aaacacgcga
     5341 aatgttaacc tcgttggcta gctcgtcggt tgaaaattca tagtcctgat gcgcgtcaat
     5401 ccactggcac agtgtgcgta acgtctgcgg cgttaagcct tttggcaagc gacgaggatc
     5461 ctgttcgttg gagctgctgc cgtggattag ctgatcaagc tcggcctggt cataatactg
     5521 atgtttttcc agcgccattt tcttttgccg ccagccggtg agcgcctctt caaagcggga
     5581 agcctggaag ggtttgatca ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt
     5641 tgccgcatcg gctgcggagg agatgacaat cacatcactt ttgcaacgcg cgttatgcag
     5701 gacaggcagt aaatcgagcc cgttctcttt ttgcatatag atatcgagca atatcaggtc
     5761 gataggcgta tcgctattga agataatctc tttggctttc tccagcgtcg aggctgttcc
     5821 acagcattga aagcctggga tttgtgctac gtatcggcga ttcagctccg cgaccattgc
     5881 gtcgtcatcg ataattaata cattgatcat ctgttcgacc tctccccgtc ccagggtatc
     5941 tggacaaaaa attgtgtgaa aatcccgggt tccgattcca cggcgatgct gccgccgaga
     6001 ttttctacct gttgtttgac aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag
     6061 acacctttgt caaaaatgtg atcgattttg tcgggtgcga tccccggtcc atcatcgtta
     6121 acttcgcagt gcagccagcc gtgacggtag tgcaatgtta cgctaatttc gccaccgggt
     6181 tccggcccta atgcttccag cgcgttttct atcagatttc ccaatgtggt aatcagcgtc
     6241 gcgacctggt cctcactgcc gctgtctggc agctggcttt cactgtttaa aatcagcgta
     6301 tggcctaaat cggtcgcgcg attaatcttg ctgattaaaa aaccagcgat aaccggagat
     6361 ttgatcttac ccagcagaga gccaatctct tcctgatagt tattggctgt tttgagaatg
     6421 taatcttcca actgcttata actcttcaga tgcaataatc cgagaatcac atgcaattta
     6481 ttcataaatt cgtgggatcg ttcacgaagt gcatcagcat agttgaccag accgtcgagt
     6541 cgctgcatca gtttacgtac ttcagttttg tccctgaagg ttgaaatggc accgatgata
     6601 acgccattac tgcgcaccgg aacggtgttg atcagtaata gccgatcttt aatcgtaatc
     6661 tcttcgtcgc ggcgcggggt accgtcgcgt aacacttccg agacatctac cacctgtgac
     6721 cacgagtggc ttagcgtcga cagtttctca tcgtcctgcg acttacggta attcagcaat
     6781 tcttgtgcgg catcgttgat cagcgtgacc tcgccgcgat cgtccacggc aacgacgcct
     6841 tctttgatag actgcaacat ggcctggcgt tgctcaaaca gcgtggagat ttcgtagggt
     6901 tctaggccga aaaggatttt tttcagtact ttaaccagaa tgcaggtgcc aatcagtccg
     6961 accagcatgc caaataatac tgaccagata atgctccagc gactgtcatt gatctgttgg
     7021 gtcacgcggc ttaactcaag gccgatcgcc accacgccaa tttgtttatg attttcatcg
     7081 tagatggggg taaatacgcg taaagcctgc gccagaaaac cgcgattgat agcgacattt
     7141 tcttcgccat tcagcgcttt aaggatgtca tcacctttaa atggctgacc aatacgctgg
     7201 gcttcaggat gcgaatagcg aagactttgc atatcggtaa cgacaataaa cagcagatcg
     7261 ttgcgtttgc gcacggcttc cgcgatggcc tggattccac tctcctgcgg ttttttctgc
     7321 aagccctgac ggatttccgg cgagtcggcg agggtacgcg ccactgccag tgccttgttg
     7381 gccagcccgt ctcgcgtcat atcactgatt tgcgagaagt aaatcagatg caccaccaat
     7441 agcaccgaga acagtaccgc gctgaccatt aagatcactg tggtactcaa tttcatcgga
     7501 cgtttgcgta acatgcggta gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt
     7561 tcttaagcat tatctctgat gaggcgggta attcaaaggg agtaagaatg attggctata
     7621 taggggaaga gactctggca acggaaactg ccagtgctgt atgaagattc cagggctatg
     7681 cttatagcga taatcatact gatgagagag ggaaagtcat ggatcaggcg ctactggacg
     7741 agggttatcg ctgttatacc ggcgaaaaga tcgatgtcta tttcaacact gcgatatgtc
     7801 agcattctgg caattgcgta cgtggcaacg tcaagttatt taatctcaaa cgaaagccgt
     7861 ggatcatgcc ggatgaagtc gacgtcgtca ctgtggttaa agtgattgat acgtgcccga
     7921 gcggcgcgct gaaataccgt cataaataag cgagggtaaa atggaaatac gcgaaggcca
     7981 caataaattt tacattaatg acgaacaagg caagcaaatc gctgaaattg tttttgtgcc
     8041 gaccggagag aatttagcga ttatcgaaca taccgatgtc gatgaaagcc tgaaagggca
     8101 agggattggt aaacagctgg ttgcgaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa
     8161 aattatccca ctatgcccat ttgcgaaaca cgaatttgat aaaacgcggg agtatgatga
     8221 tattcgcagt tgatgggaga gtacagattc acgatatttt tcatgctctc tgcggtgtga
     8281 tgcaggagag catctgaagg gtagggggat gcacaaagaa tgggcagaga gcgcgttttt
     8341 ttgtcccaag tcatcccctt tactgagcaa aaaaaagaat atctcctata tgagaatcat
     8401 caatcggggt taataagttt tgcgtcccca gagtgttcaa tattgatagg agtcatatta
     8461 tggaaggtaa aaacaagttc aatacttatg ttgtttcttt tgattatcca tcatcttatt
     8521 cctcagtgtt cttaaggtta agatcattga tgtatgatat gaatttctcc tctatcgtgg
     8581 ctgatgaata tgggatacca cgacaattga atgaaaactc cttcgcaata acgacatcgt
     8641 tagccgcaag tgaaatcgaa gatttaatca ggcttaaatg cttagactta ccggatattg
     8701 actttgacct caacattatg acagttgatg actatttccg tcagttttac aagtagccag
     8761 gaaggataag aagaacataa aaaatggcac tattctctaa aatattaatt ttttatgtga
     8821 ttggtgtgaa catatccttt gtcattatct ggtttatctc acatgagaaa acacatattc
     8881 gtttacttag tgcattcctg gtcggaataa cctggccaat gagtctgcct gtggcattac
     8941 ttttttctct cttttaggag ctgtagttga gtggacc
//
LOCUS       DACAPB010000180         8897 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.180, whole genome shotgun sequence.
ACCESSION   DACAPB010000180 DACAPB010000000
VERSION     DACAPB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.180"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..96
                     /gene="ydcY"
                     /locus_tag="IT904_003853"
     CDS             <1..96
                     /gene="ydcY"
                     /locus_tag="IT904_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 domain-containing protein"
                     /protein_id="HAP3193567.1"
                     /translation="RLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(97..546)
                     /locus_tag="IT904_003854"
     CDS             complement(97..546)
                     /locus_tag="IT904_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP3193568.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(543..1061)
                     /gene="mddA"
                     /locus_tag="IT904_003855"
     CDS             complement(543..1061)
                     /gene="mddA"
                     /locus_tag="IT904_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAP3193569.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1242..2279
                     /gene="curA"
                     /locus_tag="IT904_003856"
     CDS             1242..2279
                     /gene="curA"
                     /locus_tag="IT904_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAP3193570.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            2477..3142
                     /locus_tag="IT904_003857"
     CDS             2477..3142
                     /locus_tag="IT904_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3193571.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     KMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(3178..5280)
                     /locus_tag="IT904_003858"
     CDS             complement(3178..5280)
                     /locus_tag="IT904_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP3193572.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYITPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            5522..6583
                     /locus_tag="IT904_003859"
     CDS             5522..6583
                     /locus_tag="IT904_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAP3193573.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDIARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLT
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(6698..8197)
                     /gene="ansP"
                     /locus_tag="IT904_003860"
     CDS             complement(6698..8197)
                     /gene="ansP"
                     /locus_tag="IT904_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAP3193574.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            8463..>8897
                     /locus_tag="IT904_003861"
     CDS             8463..>8897
                     /locus_tag="IT904_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP3193575.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLT"
BASE COUNT         2378 a         2222 c         2209 g         2088 t
ORIGIN      
        1 cgtttgcgca ccgctattgc tcatcacggt cgcaagcata aagaagatat ggaagcgcgc
       61 cacgaacagt taaccaaagg cggcaccatc ctctgattaa aatgagcgtc tggctaccaa
      121 ccaggcgcca accacaagta aaattgcgcc acaaattggg ccgaacaacg cccgtagtgg
      181 tacgccatga ctgcggaaga tatccagcat cagaccgcca attagctggc tggcgaccag
      241 tactgcaata gttgtcgccg ctcccacatt ctgatatccg ctgatactgg cgaagacaaa
      301 aaacgatccc agcaagccgg gtataagtgt ccaccagcgc acgctggaca ccagttcgcc
      361 aaaccccgcc atgccttgtt taaaccataa aatagaaaca aagagcacaa tccccaccag
      421 tgagttcagc aacatggcga tgagaatggt agaggaggtc tgggtgatgc gcaccatcag
      481 cgtgttctgg actaccagac caattccggc ggcaatcaaa aaagcaaggg tgagcgactg
      541 attcatccga tcgcgtccgg ttcagtgcgt tcgtcgagtt gcaactgcat aaatgtcaga
      601 tccagccagc gcccaaattt agtgcccacc tgcggcattt gtgcggtgac gacaaatccc
      661 agcgaatggt gcagatgcag cgaggcctga ttttgcgatt cgatcccggc gaccatgaca
      721 tgcttcccgc aatcccgcgc ttcatcaatc aatcggctta acaatttacg ccccagacct
      781 ttgccctgat gatcgggatg gacataaacc gaatgttcca cggtatggcg aaaaccatcg
      841 aaactacgcc agtcgccaaa cgaggcatat cccgtcacta cgccatcttc ttcacttacc
      901 agcaccggat aacctgctat ggtccgcgct tcaaaccagg caatgcggtt atctgcatca
      961 accgtttggt cattccagat agccgccgta tacaacacgg cgtggttata aatttccgca
     1021 atggcagcac aatcggcttt gcgggcaaaa cggatggaca tgtctggcct cgataaaata
     1081 atgatgatga tgttatcaat actatcgaaa acagcccgaa gcaacggatt cctcgatgaa
     1141 attattgcgt gttgcagagg ggatgtgacg gctgcaaaca aaaccaatcc cgtgagcctg
     1201 ctactattga tggattcatg ccaaaagcac aggagagcat tatggggcaa caaaagcagc
     1261 gtaatcgacg ttgggttctg gcctcgcgtc cacatggcgc accggttccg gagaatttcc
     1321 gtcttgaaga agatgatgtc gccacaccgg gtgaaggaca ggtgttactg cgcacagttt
     1381 atttgtccct ggacccgtat atgcgtggac gtatgagcga tgagccatct tattcaccgc
     1441 ctgttgatat tggcggcgtg atggtcggcg gcacggtgag ccgtgttgtg gagtcgaatc
     1501 atcctgatta tcagcctggc gactgggtgc tgggctacag tggatggcag gactatgaca
     1561 tatccagtgg tgatgatctg gtgaaacttg gcgatcatcc gcaaaatcca tcgtggtcgc
     1621 tgggtgtgct ggggatgcca ggctttaccg cttatatggg gctgctggat atcggtcagc
     1681 ctaaagaggg cgaaacgttg gtggtagctg cggcgacagg tcctgtggga gcaacggtgg
     1741 ggcaaatcgg caaacttaaa ggttgcaggg tggtgggggt tgccggtggc gcggaaaaat
     1801 gccgccatgc tatcgaggtg ctgggcttcg atgtttgtct tgatcaccac gcggatgatt
     1861 ttgccgaaca actggcgaaa gcgtgcccaa aaggtattga tatctattat gaaaacgtgg
     1921 gcggtaaggt atttgatgca gtgctaccat tacttaatac atctgcgcgc attcccgttt
     1981 gtggcttagt gagcagctat aacgctacag agctaccacc cggtccggat cgtttacccc
     2041 tgttgatggc aacggtgctg aaaaaacgta ttcgcttgca agggtttatt atcgctcagg
     2101 attatggtca ccgcatccat gagtttcaga aggagatggg gcaatgggtg aaagaggata
     2161 aaatccacta ccgcgaagat attactgatg gtttagagaa tgcgccacag acgtttatcg
     2221 gcctgctgaa gggtaaaaac ttcggcaaag tggtgatccg cgtggcgggt gatgattaaa
     2281 gtttcaacgg cggcgtaagc cgccataaaa atccaggcaa aaagcttatt ttcagcttta
     2341 attaactaac cacgtcattg ttctgctgct aataacaaca aaggtgagtg gtttatgatt
     2401 attcacttta atacaccagg tgaactcctt ctgccatgca ggcagggttg gacagaaaac
     2461 acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa catgtcagtt tgactctgca
     2521 ggttgagaac gacctgaaac atcagcttag tattggcgca ttaaaacctg gcgcacgcct
     2581 gattactaaa aatctggcgg agcaattagg tatgagtatt acacctgtgc gtgaagcatt
     2641 attacgtctg gtttcggtga atgcgctttc tgtcgcacct gcacaagcat ttacagttcc
     2701 ggaagtgggg aaacgtcaat tagacgaaat caatcggatc cgctacgaac tggaattaat
     2761 ggcagttgct ctggctgttg aaaacctcac cccgcaagac cttgcggaac tccaggaatt
     2821 gctcgagaaa ttacagcagg cgcaagaaaa gggcgacatg gaacaaatca ttaatgtaaa
     2881 caggctattt cgcttagcga tttatcatcg ctcaaatatg cccatcctgt gtgagatgat
     2941 tgagcaactg tgggtcaaga tggggcctgg tttacattat ctctatgaag cgattaatcc
     3001 agcggaatta cgggagcata tagaaaacta tcatctgtta ctcgctgcgt taaaagcaaa
     3061 agacaaagag ggatgcagac attgtcttgc tgaaattatg caacaaaata ttgctatttt
     3121 ataccagcaa tacaatcgtt aaagtaaatg cccggcaaat accaggcatc aaggtaatta
     3181 ctcaaatcgc catgcgatat tcacgccgac accataattt cgcccgggcg caggttcgta
     3241 atatcgcccg tttgactcat tgacaatgac agaaccaacg tattctttat cgaataaatt
     3301 atcgacacga ccaaataaat ccacagttaa attgtggtaa ttgtatttat acccggtgaa
     3361 taagccgatg agagtataag acggcgcctt ggccgtattt tcatcatctg ccataatatc
     3421 gcccatataa cgcgcttccg tgcctgcata ccagccatct tccggtacat aacctatcga
     3481 cgcaaagccc atattacggg cgatcccagg catccgatta ccgttacaat cctgttcatt
     3541 gcaaacattg ctgcgatagg tcgcatcaag ccaggtccag gacgcgttta cgcgaaaatc
     3601 tcccgcgaaa cgttgatccc atgccagttc agcgccttga cgacgggtct ttccagcatt
     3661 tttgtaagtc gtacgcccac cgctactgct atcgacaaca atttcatcat cggtgtcggt
     3721 ctggaacaat gcgagactaa gcagcccatc accaatacgc gttttactgc cgatctcaat
     3781 tgtatcgttg gtggatggtt ttaaaccaaa gttcataccg ctttgcccat cagcccgata
     3841 agacagctca ttaatcgtcg gcgtttcaaa accacgcccg gctgccagat agatattcca
     3901 ggcatcggtc attgcatatt ttaacgatcc ggctggtagc catttgtgat aactggcatc
     3961 accgctgtca tcgccgttac ccggagtaat gtaatggtcg ttggaatcaa accacacgga
     4021 gctgtagcgc acgccagcat ccagcgacag tttttcgctc agctgccact gcgtctgtaa
     4081 atagggatcg atgttccaca tcagattgcg ttcgtcgcga cgcaactcac ctttttgccc
     4141 gtactccggc atgccgctat tcaggcggaa gttattgtag cccttgcggt tttcactcat
     4201 gttttcgtag ttcaggccgg tagtgaacgt gaccggaacg cccagttcac cacggtgtgt
     4261 ccagcggctg tctattccct ggtaatggcg ttgcagggta atcacgccgc ccgcatgtga
     4321 cgggttaagt tgtggtgcca tgggtattga ctggtactgg gtcgtttctc gctctccggc
     4381 atacatcatc acactcatat catcccgcga actcaggcta cgttcatagc gcaacccagc
     4441 ctgagtttgc ttgatggttt ttcgcgtgtc gtattgttca gcgcgtggcg cttgttgcgg
     4501 attcgctttc cattctgctt tggttagccc accagggtca tctgctttga tatccacgct
     4561 attgaaaatc aggcttaatt tgctggcgtc atcaatgcgt acgcccagtt tggcattagc
     4621 taaatttttc tgtgcgccac tatggtcacg atagccgtgg gtcgtaaaac gcgtggttga
     4681 gacggtgtaa tcgacatcgc cgggctgtgt gccgtcgccc gttgcacccg ttgctttgag
     4741 cccatagcgc cagctgccaa aactgccgta gtaactgctg gcttcaatgg ttggtggctg
     4801 ttgcccggtc tgggtggtga cattcattac accgccagac gcgttgccat acagggcaga
     4861 gaaggggcca cgcagcactt ccacattttg cacactgctt aaatcgatgt tggatgtttg
     4921 cccttgcccg tcgggcatgg tagcgggaat accgtccaca tacaggcgaa taccgcgaat
     4981 tccgtaagtg gagcgggagc caaatccgcg aatcgacagc tgtaaatctt gcgcatagtt
     5041 ctgccgattt tgtacctgca aaccaggcac gctggtcagt gattcggaca agttaatgcg
     5101 cggtgttgcc aggcgcatct cctcgccatc caccacgctt actgctgctg gggtatccag
     5161 ttctgaaacc acctgcggtg cggcgctgac aatcatggtc tgttcatcag cggcaaaaac
     5221 aacgggggaa agggcaagta gtgcgggcaa aacggtctgt cggacggaaa aaatcttcat
     5281 gaaaaaagcc aggttaagaa tgggaaaacg ccgttatggt aatgaaattg taaatttatg
     5341 gaaaatgaaa cggcacgata cgttaagtaa ttgagaaaat tgtagtcgta acggcaagaa
     5401 atgctccaca tttgagaaaa taatgattac cattcccatt tataacaaga gcgtaacgat
     5461 gattacgctt agcgaagcat tgtgaagcag caaaaatatc ggttcatcaa agggagtcgt
     5521 catgcattta cgtcatctgt tttcatcgcg cctgcgtggt tcattactgt taggttcatt
     5581 gcttgttgct tcatcattca gtacgcaggc cgcagaagaa atgctgcgta aagcggtagg
     5641 taaaggtgcc tacgaaatgg cttatagcca gcaagaaaac gcactgtggc tcgccacttc
     5701 gcaaagccgc aaactggata aaggtggcgt ggtttatcgt cttgatccgg tcactctgga
     5761 agtgacgcag gcgatccata acgatctcaa gccgtttggt gccaccatca ataacacgac
     5821 tcagacgttg tggtttggta acaccgtaaa cagcgcggtc acggcgatag atgccaaaac
     5881 gggcgaagtg aaaggccgtc tggtgctgga tgatcgtaag cgcacggaag aggtgcgccc
     5941 gctgcaaccg cgtgagctgg tagctgatga tgccacgaac accgtttaca tcagtggtat
     6001 tggtaaagag agcgtgattt gggtcgttga tggcgagaat atcaaactga aaaccgccat
     6061 ccagaacacc ggtaaaatga gtaccggtct ggcgctggat agcaaaggca aacgtcttta
     6121 caccactaac gctgacggcg aattgattac catcgacacc gccgacaata aaatcctcag
     6181 ccgtaaaaag ctgctggatg acggcaaaga gcacttcttt atcaacatta gccttgatat
     6241 cgccaggcag cgtgcattta tcaccgattc taaagcggca gaagtgttag tggtcgatac
     6301 ccgtaatggc aatattctgg cgaaggttgc ggcaccggaa tcactgactg tgctgtttaa
     6361 cccagcgcgt aatgaagcct acgtaacgca tcgtcaggca ggtaaagtca gtgtgattga
     6421 cgcgaaaagc tataaagtgg tgaaaacgtt cgatacgccg actcatccga acagcctggc
     6481 gctgtctgcc gatggcaaaa cgctgtatgt cagtgtaaaa caaaaatcca ctaaacagca
     6541 ggaagctacc cagccggacg atgtgattcg tattgcgctg taatagatat ggcgagggtg
     6601 aaaacggaga gacttgcctg acttttatca cgccgcatgc ggcacacctt cgtcagtaat
     6661 ttgaagttgc ccggcacgta gccgggcaat tttgcaatta gctcgccgtt tcaggcttaa
     6721 acacgatttc ctgtttttct tcatcttcct cgacgactgg cgcagtgctg tgaatttcag
     6781 caacgcgttt gcgcacacca aaccagccta taaccagcag aataccgata atcggcagcg
     6841 ccgcgatagt ataagtcccg ttcgggtaat cgaacgccat cagcacaagg acactcagta
     6901 aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg cagtttaaaa ctgacatccg
     6961 ctgctttgcc ttctttaatc gctttacgca ggcgcatctg gcacacgatg ataaacgccc
     7021 atgaagcgat gattcccagc gacgcgaagt tcaacacaat ctcaaatacg cgcgacggca
     7081 ccagatagtt gaggaatacg ccgacgacat acacaactag tgtcgctaga atcccggcat
     7141 acggcacatg ctgacgactc attttcgcca taaaactcgg tgcggaaccg cccatcgcca
     7201 ttgagcgcag aatacgtccg gtgcagtaca gacctgagtt caggctggag agggcagcgg
     7261 tcagcaccac aatgttcata atgctgccga tatatggcac acccagttta gagaaaaacg
     7321 tcacgaacgg actttgcccc gcctgatacg cgctccacgg caatagcata accagcaaca
     7381 ccacggagcc gacgtaaaac aggccaatac gccaaatcac gctgttaatc gctttaggca
     7441 ccatggtctg cggatcttta cattctccgg cagctgtacc caccatttca atggaggcaa
     7501 aagcaaacac tacgccctga atcaacacca gagcaggcag caaaccgtgg gggaagaagc
     7561 cgccattatc ggtaattaaa tgaaagccag tggtgttgcc atccagcggc tgaccactac
     7621 cgaggaacac tgtacccacg accaaaaagg tcacaatggc gagcacttta ataagcgcaa
     7681 accagaactc catctccgca aaccatttca caccgatcat attcatggtg ccaacgatgg
     7741 taagtgcagc gagcgcaaag acccactgcg gcacgccgcc aaacgcaccc cagtaatgca
     7801 tatacagagc gacggcggta atatcaacaa tccccgtcat cgcccagttg atgaagtaca
     7861 tccagccagc aacataagcg gctttctcac ccaaaaactc acgggcataa gagacaaaac
     7921 tgccactgga agggcggtgt agcaccagct cacccaatgc acgcagaata aaaaacgaaa
     7981 acaagccaca aattaaataa accagtgcca atgctggacc cgccatttgc agtcgggctc
     8041 ctgcacctaa aaacaagccg gtgccaatcg cgccgccaat ggcgatcatc tgtacctggc
     8101 gattgcccat cgctttgtga tacccctctt cgtgggcatt aagccagcgg cgtttcgcgg
     8161 cgtgttgatc tgaagtgtcg gtgtcgtgtt tactcattgc tctccctgat tgctttaatg
     8221 aaaaagtcat ataagttgcc atgaacaatg tttattcatc ctggcgatga tagttatcac
     8281 cattattctt tatgggtata aaactatcgg cgcagcatcc tacccgttat cgataaacga
     8341 tgcaaaacat ccccttacaa tcctgaaggg gaataataca actgacgaaa aaatgacaaa
     8401 tctttttgct ggttaacctg tgtactgtcc tacacttaat ctttaaaaga ttgtgagggc
     8461 atatgattaa ggtgtatggc gtacccggat ggggctctac aatcagtgag ctgatgctaa
     8521 cactggctga tattccttat cagttcgttg atgtcagtgg ttttgaccac gagggagctt
     8581 cacgcgagtt attgaaaacc ctgaacccgt tgtgtcaggt tccgacactg gcgctggaaa
     8641 atgacgagat tatgacggag acagcggcga ttgcatttat ggttctcgat cgtcgcccgg
     8701 accttgcgcc accggttggg cgcgccgaac gtcagcagtt tcaacggcta ttggtctggc
     8761 tggttgctaa tgtctatcca acattcactt tcgccgatta ccctgagcgt tgggcatctg
     8821 acgcacctga acagttaaag aagaatgtaa ttgaatatcg caaatcactt tatatctggc
     8881 tgaattcaca attgacc
//
LOCUS       DACAPB010000181         8859 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.181, whole genome shotgun sequence.
ACCESSION   DACAPB010000181 DACAPB010000000
VERSION     DACAPB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.181"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..556)
                     /gene="motA"
                     /locus_tag="IT904_003862"
     CDS             complement(<1..556)
                     /gene="motA"
                     /locus_tag="IT904_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP3193576.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVV"
     gene            complement(683..1261)
                     /gene="flhC"
                     /locus_tag="IT904_003863"
     CDS             complement(683..1261)
                     /gene="flhC"
                     /locus_tag="IT904_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAP3193577.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(1264..1613)
                     /gene="flhD"
                     /locus_tag="IT904_003864"
                     /pseudo
     CDS             complement(1264..1613)
                     /gene="flhD"
                     /locus_tag="IT904_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
     gene            2393..2821
                     /gene="uspC"
                     /locus_tag="IT904_003865"
     CDS             2393..2821
                     /gene="uspC"
                     /locus_tag="IT904_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAP3193578.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(2828..4252)
                     /gene="otsA"
                     /locus_tag="IT904_003866"
     CDS             complement(2828..4252)
                     /gene="otsA"
                     /locus_tag="IT904_003866"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAP3193579.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(4227..5027)
                     /gene="otsB"
                     /locus_tag="IT904_003867"
     CDS             complement(4227..5027)
                     /gene="otsB"
                     /locus_tag="IT904_003867"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAP3193580.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(5194..6183)
                     /locus_tag="IT904_003868"
     CDS             complement(5194..6183)
                     /locus_tag="IT904_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAP3193581.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(6195..7709)
                     /locus_tag="IT904_003869"
     CDS             complement(6195..7709)
                     /locus_tag="IT904_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAP3193582.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(7779..8768)
                     /locus_tag="IT904_003870"
     CDS             complement(7779..8768)
                     /locus_tag="IT904_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3193583.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         2273 a         2286 c         2047 g         2253 t
ORIGIN      
        1 gaacgacccc cattacagcc gcaacaatac caaacgccgg aagtgagtcc ccgaccagcg
       61 ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca
      121 gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga
      181 taaaatcaag catgacgcta tccgcgagga tgcgtgggta gctggcgaag atctcgctct
      241 cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca
      301 tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac
      361 gaaacagcaa cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga
      421 taaacgaccc aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc
      481 caaggcttcc accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac
      541 ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt
      601 caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga
      661 atgttgcgcc tcaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg
      721 ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggtt
      781 gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc
      841 cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc
      901 aggcacgggt taatgccagc agtggtcctt cttctgcttg tgggcactgt tcaaggtata
      961 aacggtaggc tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact
     1021 gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg
     1081 ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta taaagtttta
     1141 tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg
     1201 tgatcaattc cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca
     1261 tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg
     1321 tgttgagagc atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt
     1381 caactgagta atcgtctggt ggctgtcaaa acggaagtga caaaccagtt gattggtttc
     1441 tgccagctta accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt
     1501 tatgccgaga cgaacatagc ggacgctttg tcctgaacaa tcaaacgctg tgcaagtagt
     1561 aaatatgaca agttgatgtc ataaatgtgt ttcagcaact cggaggtatg cattattccc
     1621 acccagaata accaacttta tttttatgcg gtttcaccgc accccgtgat gtcgccggga
     1681 agccccggta aaaaataatt agcattagaa tagttgcgat aagctgcaat aagcagaacc
     1741 acctttttgg tttaatatgt ccttacaaat agaaatgggt ctttacactt atctaagatt
     1801 tttcctaaat cgacgcaact gtactcgtca ctacacgcac atacaacgga ggggggctgc
     1861 gattttcaat aatgtgtgat gcagatcaca caaaacactc aattacttaa cataaatgta
     1921 taagtcatac ttttgttttg ggtgtatttc aatctgttaa aaagtttttc gctacgctag
     1981 caagcaaaaa tgaaacagga ataatcgaaa tgggatgttg cgcacagtca aaataactca
     2041 ccgtaaataa tcatctgcta taaataatca ctttcatgca ataccagata agctattttt
     2101 aaacagacac ttaccgcaca acaaactaat taaatacaaa gaaaggcaac aatcaactta
     2161 atgttaatga aatgttttac atatattaac cacttgttta atgcggagaa tcagtacact
     2221 attttcttat aattacattt gaaattatat gatcaccggg agtgattgat taattgtctt
     2281 tactaataat cagactaata tttaccagtt tgaccgagtt gggattgcgt cgtttctcca
     2341 ttaggagtaa agctttaatg tcacctgaag ttcacagaat aaggaacagg ttatgagcta
     2401 tagcaatatt cttgtcgctg ttgcggttac accggaaagt cagcaactgc tggcaaaagc
     2461 ggtatctatc gccaggccag taaagggaca catcagttta attactctcg cttccgaccc
     2521 ggaaatgtac aatcaattag ctgcgccgat gcttgaagat ttacgcagcg tgatgcagga
     2581 agaaacgcaa agctttcttg ataagttaat tcaggatgcg gggtatcccg ttgacaagac
     2641 gtttattgcc tacggtgagt taagcgaaca tattctggaa gtatgtcaca agcatcattt
     2701 cgatttggtg atttgcggta atcacaatca cagtttcttt tcgcgagcat cctgctcggc
     2761 gaaaagagtt attgcctcaa gtgaggtcga tgtgctgtta gttccactta cgggagatta
     2821 accgctccta cgcaagcttt ggaaaggtag caactttatc gcgctgctgg ctttccgcgc
     2881 ttcgcggaac tatctgcttt aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt
     2941 ttttcacgat aacgtccagc atttctgcat gacgggaaat acgttccgcc agcgacatag
     3001 tcaatgcacg atccagcgca gctgcaactt cgtcacgatc gtaggggtta acaattaacg
     3061 ccgacgttaa ctcgtttgcc gctcccgcaa attgcgaaag aacaagaacg cccggattgg
     3121 ctgggtcctg agcagcaaca tactcttttg ctaccaggtt catcccgtca cgcagtggcg
     3181 tcactaagcc cacgtcagag tagcggaata ttttcatcag taatttacgg tcaaaatgct
     3241 gattcaaata ataaagcggc gtccagccta attgcccgta tttaccatta attcgtccag
     3301 cttcattttc gagctgatga cgaatatcct gataggcttg cacatcacca cgcgacgttg
     3361 gtgcaatctg ggtataacga attttaccat gatgctgcgg atatttttcc agcaacgctt
     3421 cataggcgag aaaacgctct ggcaaacctt tggaataatc cagccgttcg acagaaaaga
     3481 tattttgtac gtttttcagt tccgctttaa gttgcgccag ttttggcggc agtggcccgg
     3541 cagcctgttt ggctatttct ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg
     3601 ctttgcccca ggctgtatgg ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa
     3661 gacaatccag gaacgccaga cgatcgtttt ctgtctggaa acccagcaaa tcataatcac
     3721 aaagctgttc aagcaaggtg tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga
     3781 aaggaatatg cagaaagaaa ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg
     3841 caaatggcaa caggtgataa tcgtggatcc agataatgtc atcgtcttgc aacagcggca
     3901 gtaatttatc tgccagcaac gcatttacgc gtagatagcc gtcccaggca ggacgctgaa
     3961 attgcaccag atcgagccga taatgaaaag cgggccagag aacggcattg gagaattggt
     4021 tgtagtattc gtcaaggtcc tgttcgctga ggttaaaaga ggcccacgta atgttacctt
     4081 ttttcacctt ttttagcggc tgatcctcat tccctgtttc accactccag ccaaaccaca
     4141 gtccgcctgc ggctttcagt gcccccagta tgccaacggc aaggccaccg gcactggcgg
     4201 cgtgctcgtc tggtggtgca atccggttag atactacgac taaacgactc atagtcatca
     4261 ctcctgttat tttctctttt ttgttgtaat gcggtggtta tcatttcaag ccagctccag
     4321 acatccggca cacccgccag tcgccatgat gcttgagttg cacctgtgcc aatttttact
     4381 gacattccgc ccagtcggtt aacgactgcg aagccagatt catcggttaa atcatcgccc
     4441 agaaatacgg gcgttcgccc gataaaggga gcttcctgca taaaagctgc aattgcctca
     4501 cctttactgg tacctctcgg tttgatctcg acaacacact ttccctgctg taacgccatt
     4561 tgtggccaga tctgagtaat acgttgcgct aatgtcatta atgcgtcttc atgctgcgga
     4621 gcctgacgat aatgcagcgc aaaagccatc cctttcgcct ccagctccgc gccgggatac
     4681 tgagcgatga ctgtatgcag ttgcacgcta atatcacgcg caatcgcatc cggcagatga
     4741 acgatatgtg ttttaccatt gatgtcacgg cgctccgccc catgcacgcc cgctaacggg
     4801 aagcgataag gttttgccag tgcgtcaagc tccaccattg agcgccctga tatcaatgcc
     4861 aatgcaccat cacttgcggt tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg
     4921 acgacctgat cgggatgcgg tttgatttcc gccagcgttc catcaagatc aaaaaaccag
     4981 gcatatttcg cggatagttc aggggtttcg gttaacggtt ctgtcacccg gttctcctcc
     5041 ttctcttttc ttattgtgtt gcgcagaacg cgctttacag acatgtaagt atagacagtg
     5101 tgacgggggt cgccatttgc aacgaagaac agccaaccgt tatggcggtt ggctatggtg
     5161 ggaaaaaacg ctaaattgtt gcagaaaaaa gcatcagaca gtgcgtttcg ctttttgctt
     5221 gtaacggtcg aagatcaccg ctgccagcag gattaagccg cgaaccacgt actgcgcgaa
     5281 aggagaaata ttaagcaggt tcatggcgtt ttccacggtg cctaaaatta agatacccgc
     5341 caccacatat gagatttttc cgatgccacc tttcagagaa acgccaccta aaacgcaggc
     5401 ggagataaca atcagctcat aaccaatcga cgtcattggc tgcccactgg tcatacgtga
     5461 agccagaata attccggcta tcgctgatac caggcctgag agaacaaaga taataatttt
     5521 ggtgcgaaca accggtacac ccgccagacg cgcggcctct tcgttcccgc caatcgccag
     5581 ggtgttacga ccaaaggtgg ttttattcag cagcaaacca aagataatca gacacgcgac
     5641 ggtgagccag attggcgcag gcagaccgaa ccagttggcg taaccaaggg caaagaagct
     5701 ttcatcttcg ataccgaccg ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat
     5761 ctgcatcgtt gccaatgtcg tgatcagagc atttattttc agtttggcga taacaaagcc
     5821 attgaccagg ccacagagaa cgcccagcaa caaccccgct gccacgccaa tccacaggct
     5881 ttcagtcagg ttaataacca ccgccgtggt gacacccgca caggcaatta cggaggcgac
     5941 agaaaggtca aagtcaccgg aagcgaggca gaacaacatg ccacaagcca ccatccccga
     6001 catggaaatt gccaggccca accctttcat attaatgaag gtggcaaaat ttgggacaaa
     6061 aatggcacag gcgataaaga gcaccgcaaa caccaccagc atgccgtact gatcccagat
     6121 acgcccgaag ctgaatgacg acttaggtgc gccagacccc gatgtagaaa cagaagacat
     6181 catactctcc ttactcaggc aacagcctgg ctgactttag gcatcgcaag gctcagtgcc
     6241 tgacgctcat ctgcctgctc gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc
     6301 acaatccggt cggcaacgcc gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc
     6361 acgccctgcg ccgccagcgc ataaattacg ttatatattt cgtgcttagc gccaacatca
     6421 atgccgcgcg taggttcatc cagcaaaatg accttcatct cttccgataa ccagcggccc
     6481 agaatggctt tttgctgatt tccgcctgag agattcatga tcagttgctc cgcgcccggc
     6541 gttttgatgt tgagcgaacg aatgtggtga tcggcattgt tttcttccca accgttgttg
     6601 attacacaac cgccgagcac atgtttacgt ctggcactga tgttgatatt gtcgcgaacg
     6661 gagtgcacgg gaataatgcc ttccgctttg cgatcttccg ggcagagcat catgcctgcg
     6721 gcaatggcgt ggctcggttt acgaatatcg atcggctgtt ggtcgatata aacctgaccg
     6781 gcggtgattt gcgtcccgcc aaacatgcct ttcattaatt cgctacgccc cgcccctacc
     6841 agaccaaaca gcccaacaat ttcaccactg cgaaccgcca gacttattgg cgtacgcacg
     6901 cctggtgctt tcacagcatc aagacgtagg cgctcctcgc cataactacg cggttgccag
     6961 ccgtagatat cgccaatgtc gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc
     7021 tgctgcatat cggtaaaggt tttgacataa cgtccatctt taaagacagt aatggcatcg
     7081 ctgagggcaa atatttcttc catacggtga gaaacgtata agattacccg cccctctttt
     7141 cgcagttcac gaataacgcg gaaaagattg tcgatttcac gggcagagag ggagctggtt
     7201 ggctcatcaa aggcgataat tttggcgtta cgcgccagcg ctttggcgat ttcaaccatc
     7261 tgccactgac caatggagag atatttcagc ggcgtgtccg ggtcaatatc cataccaaga
     7321 tgtttaagtt gtaaacccgc ctcataattc agcaatgagc gattcacaat gccgccttta
     7381 tgcggcagct ggccgagata gatgttttcc gcgacggtca tttccggcac gagatgcagt
     7441 tcctggtaaa taatcgccac gcccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt
     7501 tcctgcccat taatcactac agaacccgtg gttggcgcat agttgccgct gaggattttt
     7561 aagagagttg attttcctgc gccattttca cccatcaacg catgaacctg accggcatag
     7621 cagtcaaaac taatatccgt cagcgcctta acgccgggaa acgttttacc gatgccgcga
     7681 aatgagagat acggggtaga ctgttgcata acgtctccgt gaatcactgg tcgttcacac
     7741 cccctctgca tcatgcagag gggaattttt ccggcaaatt acttaccgcc taaacctttt
     7801 ttctccagtt cttctttaaa gttgtcacgc gtgatcagta ccacgtcggt aacttcggta
     7861 aattttggcg gttcaacgtc ttttgctacc cagttgtaaa gcatttcgct ggatttatag
     7921 ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc cggttgcctg tgctttagac
     7981 agttcgctca ccgcatccac accgttaatg ccaatgccga tgatatcggc cgctttaaag
     8041 ccctgacctt ccgtcgcgcg tacgccgccc agcacggtgc tgtcgttcat accgacgatc
     8101 agccaatgtt taacttccgg atgttgaacc agcattgagt tggcagcgtc aaatgccccc
     8161 gggatgtcgt tagatttggt aggtacctga taaatttgtt tttccgggaa tccggccgct
     8221 ttcagcgcat ccatagatcc cgtagtacgg cggcgggcgg tatccagttc gttggcggta
     8281 atcgccatca ccgcgctttc tttgacatcc cagccacgtt tctgcatctc tttatacagt
     8341 tcctggccct gacgttcgcc aattttagtc gccgccatca tcaccagcgg aacggtatcc
     8401 attggcttac ctttggcgtt aacaaactgg tcatccacgg caatgacttt catatcgtag
     8461 ccacgcgctt tcgcgacgat ggcagagccg agtttggggt ccggagtaca aataacgaaa
     8521 ccttttgcgc cactggcagc caggctgtcg atcgcgttca atgttttttc gccatccggc
     8581 acggcaatct taataacctc aaaccctaaa tccttcccgg ctttatcggc aaacttccat
     8641 tcggtctgga accacggctc ttccggttgc ttcaccagaa aaccgagctt caggttctcc
     8701 gccatagcgg attgtgacat aacggctgcc agaccaatgg ctgccagggc tttagtaaat
     8761 ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta gggcaaaaac gaatgacatt
     8821 cgttaaatta atcggaaaac aaagcattac cttttaact
//
LOCUS       DACAPB010000182         8677 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.182, whole genome shotgun sequence.
ACCESSION   DACAPB010000182 DACAPB010000000
VERSION     DACAPB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.182"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            263..505
                     /locus_tag="IT904_003871"
     CDS             263..505
                     /locus_tag="IT904_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP3193584.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(537..1187)
                     /gene="tetR(A)"
                     /locus_tag="IT904_003872"
     CDS             complement(537..1187)
                     /gene="tetR(A)"
                     /locus_tag="IT904_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAP3193585.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1293..2492
                     /gene="tet(A)"
                     /locus_tag="IT904_003873"
     CDS             1293..2492
                     /gene="tet(A)"
                     /locus_tag="IT904_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAP3193586.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2459..2647)
                     /locus_tag="IT904_003874"
                     /pseudo
     CDS             complement(2459..2647)
                     /locus_tag="IT904_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2759..3064)
                     /locus_tag="IT904_003875"
     CDS             complement(2759..3064)
                     /locus_tag="IT904_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3193587.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            complement(3092..4306)
                     /gene="floR"
                     /locus_tag="IT904_003876"
     CDS             complement(3092..4306)
                     /gene="floR"
                     /locus_tag="IT904_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HAP3193588.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTYFVFFSTATRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            complement(4523..5407)
                     /locus_tag="IT904_003877"
     CDS             complement(4523..5407)
                     /locus_tag="IT904_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HAP3193589.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRR
                     GVERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDAD
                     QWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLA
                     PTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALS
                     KGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGI
                     VQGGSVSWQLGRQRGLER"
     gene            complement(5438..6931)
                     /locus_tag="IT904_003878"
     CDS             complement(5438..6931)
                     /locus_tag="IT904_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HAP3193590.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(7142..7366)
                     /locus_tag="IT904_003879"
     CDS             complement(7142..7366)
                     /locus_tag="IT904_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193591.1"
                     /translation="MKKPNQDDEPFFITEEIAAEMIAGGYEFELPPIPCTIRLRDVLE
                     RMTDAELALQPGEIADQERERCRRKPCSTS"
     gene            complement(7363..8100)
                     /locus_tag="IT904_003880"
     CDS             complement(7363..8100)
                     /locus_tag="IT904_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3193592.1"
                     /translation="MRRTKPVAAPMVARVYLRVSTDAQDLERQEAITTAAKAAGYYVA
                     GIYREKASGARADRPELLRMIGDLQPGEVVIAEKIDRISRLPLPEAERLVASIQAKGA
                     RLAVPGVVDLSDLAAEAQGVAKIVLEAVQIMLFRLALQMARDDYEDRRERQRQGIELA
                     RQAGRYKGRRADPKRRAQVVALRKSGYSINKTAELAGYSAAQVKRIWAEVSQAEAKQH
                     GAFVEDALTEADALAAVGQDERQEERA"
     gene            8427..>8677
                     /locus_tag="IT904_003881"
     CDS             8427..>8677
                     /locus_tag="IT904_003881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193593.1"
                     /translation="MRRCSSEAKKNRLGVSEAASLHAVQGGRNRAGDQGGKPRGLMCS
                     SATLAGFFDLSDASGPPWALLDLLLLFLDVLLLLVLLLVA"
BASE COUNT         1644 a         2727 c         2602 g         1704 t
ORIGIN      
        1 accggagaat atcgctggcc aaagccttag cgtaggattc cgccccttcc cgcaaacgac
       61 ccctggaagc cccgtagcga cgcggagagg ggcgagacaa gccaagggcg caggctcgat
      121 gcgcagcacg acatagccgg ttctcgcaag gacgagaatt tccctgcggt gcccctcaag
      181 tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc ttgagtccac gctagatcta
      241 tctcatctgc gcaaggcaga acgtgaagac ggccgccctg gacctcgccc gcgagcgcca
      301 ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac gagcggacgc cgcagcagga
      361 gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc tagggcaggg
      421 catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag ggggagggga
      481 tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa
      541 tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt cgccaatcca
      601 tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg
      661 tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg
      721 ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa gtagctgatt
      781 gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc
      841 tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg
      901 ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag
      961 cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg
     1021 gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga
     1081 acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac
     1141 aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta
     1201 tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat
     1261 cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacagaccc ctgatcgtaa
     1321 ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg
     1381 gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg
     1441 cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt
     1501 tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc tgctgtcgac tacgccatca
     1561 tggcgacggc gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg
     1621 gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg
     1681 cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc
     1741 tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg gcagccttga
     1801 acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc
     1861 ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga
     1921 ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg
     1981 ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca
     2041 tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg
     2101 tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag
     2161 gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc
     2221 ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggatg
     2281 aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg acctcgatcg
     2341 tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg aacgggtggg
     2401 catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt
     2461 ggagaaattc ttcaaattcc cgttgcacat agcccggcaa ttcctttccc tgctctgcca
     2521 taagcgcagc gaatgccggg taatactcgt caacgatctg atagagaagg gtttgctcgg
     2581 gtcggtggct ctggtaacga ccagtatccc gatcccggct ggccgtcctg gccgccacat
     2641 gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac agtctatcgc ttagcggaaa
     2701 gttcttttac cctcagccga aatgcctgcc gttgctagac attgccagcc agtgcccgtc
     2761 actccgcggt cttcactgcg tgatcgagtt gatcgacacc cgccgtgaca cgctccatga
     2821 agtgcctgcc tgcgtctgtt agccgaacgc cccgcgcatg gcgctcaaat agcaggacac
     2881 caaggttatc ctccagcgct ttcacacgcg cgctgacgct cgactggctg ataccaagtg
     2941 ccttggccgc atgccgaaaa ttcagatgct cggcgacggc gatgaactga acaagggaaa
     3001 tgagcggtat cctgccagac aggataccga cattcacgag gtttcgatgg ttagtgcgcc
     3061 gcatcggagc gggcctgcta ccagtcgtcg gttagacgac tggcgacttc tcggtggcag
     3121 ccccacggag ccgaaggagc accagcccca acgaaaccag taccgccatc gccgtggcgt
     3181 aacagatcac gggccacgct gtgtcaccgt ttaaaagtgc caccgccaat gtcccgacaa
     3241 tgctgactat caggctttga acgcagaagt agaacgcgac cgctgatccc gcgatgtcgt
     3301 cgaactctgc caaagcgccg ttcgcggtaa cggacaccgt gaagacaata ccgaccgcga
     3361 caacccacat cggtaggatg aaggtgagga atgacggcga gccgtaaagt tcgccgatcc
     3421 ccaacaggac cgctccgcaa acaagcaacg ccatcccacg cgccacgcat cctgcgatgc
     3481 cccatctggc gacaaaggac ttcgcgaaac gggttgtcac gatcattaca agcgcgacag
     3541 tggcgaaggc aaagctgaat ccgatctcgg aatattccgc ttggcctatg agcacacggg
     3601 tagccgtcga gaagaagacg aagtaggtgc ccataccggc gctaaagccg acagtgtaaa
     3661 cccaaaaagc cggactcgcg aagatcggca agacagatcg gcgcgtcttg acttgatcca
     3721 gagggcgggt ttcgtgccac ctgaaacccg catttaggag tgcgagcatc gccagtatag
     3781 ccaaagtaat gaatatcgcc tgccatccca agaactcgcc gatcaatgct ccggcgatag
     3841 ggccgagcgc aggcacgaac gccagcatcg aactgaaaag gccgtagatg acgacaccct
     3901 caggacggtt ggcataaacg tcgcgaaccg tcgcgaacgt cgccaccagc atggccgacg
     3961 cgcccactgc ttgaagtaga cggaaagcga caaaggccgg tgcagttgaa gaccaagctg
     4021 ctcccagaga cgcaatgacg aaagccgttg cgcccgcaag tagaattggc cgtcgcccga
     4081 ttctgtctga gagcggacca aaaatcacct ggcccacgcc gagcatcacc atatagaggc
     4141 tcaacgtgag ttggatcata gcgggcgtcg tgttcaggat gccgggcatc gctggaacga
     4201 cagggagata aatatccatc gccagtgaag cgaggatgtc gaaaggagcc atcagcagca
     4261 gtgctgccgg cagcgtatag gcccacgcgg ggcgtgtggt ggtcatgacg aatcaaccct
     4321 cgattaagga ataccgggcg acgtctgctc gtcagcaatc agatgagact agccttacag
     4381 agcgccgcaa caacaatact ggttgttgcg gcttacttgt ctgctgactt ggaatttccc
     4441 atctgattac tccacgctta cgaatatgaa ttgctacatt ttatccgatt atcttttgct
     4501 tcgcaatgcg gcatgggcgc actcagcgtt ccagccccct ctgccgccct aactgccacg
     4561 acaccgaccc gccctgcacg atgcccataa cctcgcggcc gagttggcgg tcgatgatcg
     4621 gccgccacgg gacaagggtg aactcatggg atttctcgac cacggcgaac ttgccgctcg
     4681 atagatgcac ggttccggta aacttgccgc tgacgctctc gccgtccgtg gcggcgcgga
     4741 acggcaggcc cttactcaaa gccatatccg atccgacgcc ggctacctcg cgctcccgca
     4801 ggatggcgag aaggttgcgc cggtagaaga cgcggctgtc ccggctgcgg gtggcgtcgc
     4861 cctgttcgat atggtgctcg cggcgctggt ccatggcttc gcggacttgt tgcccgaagc
     4921 cggttggcgc aaggtcggcc gtctcgccgt ggatcagccg ccggtccagc caggtcgcgc
     4981 cgtccgatcc gatctgtttg ttcagatcga ccggggaaag gacgcgaacg ctggcctgac
     5041 tgtctcggcc ggcgtcatgg gcggcggcac ggctgaccag atcatcgggg atgcgccatt
     5101 ggtcggcgtc gatccgctcg acgataccgg cccggcgtag cgcctccagc cggcgcacat
     5161 gggcatcgac atagccctca tagtcgccgc ctggaacgcg gcacgtatag gaagaataaa
     5221 cgcccttttc acccaagtcc aacagctttg gaccgcagtt gactctttcg acacccctgc
     5281 gatgcaaccc aatccggctg acggggagcc agcaacgctg aaaatttacc ctcctctttc
     5341 ccactagcgg ctccttttcc gacaaccagc acggcggatc cctgccgcgg cgctgtgaac
     5401 gcagcatttt gattggtatc gttggccttc aggctcgtca gtcaaacaga cccaggagca
     5461 gctcagccgg tggcgcccgg ctttcgggta acgccctggt cccgctggtt tcggctttgt
     5521 gcttcaggtg atcaaggatc tgcttgatca ctatagggtc ttcaatgcag gcgatgactt
     5581 tcatggcgcc gccgcagccg ctgcaggtct cgatgtcgat attgaaaaca cgcttgagcc
     5641 gttgcgccca tgtcatcgac gctcgccgtt gtgctggtgt tgccggttca tcagccaccc
     5701 tgaccttgtt gcccctgccc cgttttgccg gcgtgaccaa cgcccggtgc cgactgttgg
     5761 gtgcgaacac cccgtggaag cgggttaggt tgactctggg cttcggtacc agggcggcca
     5821 gccttgcaat gaaatccaat ggttcgaaaa tgacgtgcgt ggtgccgtcc cggtacggcg
     5881 tcttgagctg gtagcgcacg ttgccgcctc gtgttaacga cagccgcttc tcggataccg
     5941 ccgggcggct gatgtaccgg cacagccgtt cgagcttctt gcgttcatcg gccctggccg
     6001 ccacgccggc gtgcaggctg gacccggcta ccttgccaat cccgtcaccg aacggatcac
     6061 cactggtcgg cagagtttgc aaagtgaaca cctttcgccc cgcctgtgaa ccgacagcga
     6121 tacggtaagt gatcgagtgc cccagcaggg gtgtcatcgg gtcgtcatcc accgcatccg
     6181 aggccagata gctgttttcg acatcccgtt ccagcaggcc ttgccgttcc agatagcgac
     6241 ccacccggtg ggcgatggtg tgcgtcagct gggtgagctc tgggctggtc ggcgccttga
     6301 cccagcggaa acgcgctgag ccgtgggatt gctcgacata cacaccgtcg agaaacagca
     6361 tgtggaagtg aacattcaga ttgagcgccg atccaaaacg ctggatcagg gtgaccgcgc
     6421 ccgtcttggc cacttggtgg gtatggcccg ctttcttgac caggtgcgtg gcaatgacgc
     6481 ggtaaacgat gcccagcacc caccccatga tctcgggccg gctggcaaac aggaaacgca
     6541 gctgaaacgg gaagctcaac acccactgac gcatgggttg ttcaggcagt acttcatcaa
     6601 ccagcaaggc ggcactttcg gccatccgcc gcgccccaca gctcgggcag aaaccgcgac
     6661 gcttacagct gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga acccgtagaa
     6721 agccatgctc cagccgcccg cattggagaa attcttcaaa ttcccgttgc acatagcccg
     6781 gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga
     6841 tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc
     6901 ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac
     6961 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
     7021 agacattgcc agccagtgcc cgtcactccc actcgattgt aaacaagcac acaaaaccct
     7081 ttagtgacaa caatttgcaa gaactatcag gctcagtgcc atgaaaaata ccatgaccag
     7141 atcatgaggt tgaacacggc ttgcgccggc agcgttcacg ctcctggtcg gcgatctcgc
     7201 ccggctgcaa tgctagctca gcatcggtca tgcgctccag cacgtcgcgt aggcggatgg
     7261 tgcaaggaat gggcggcagc tcgaactcat agccgccggc gatcatttcg gccgcaatct
     7321 cctcggtgat gaaaaacggc tcgtcgtctt ggttcggctt cttcatgccc tttcctcctg
     7381 gcgctcatcc tggccgacag cggccagggc atcggcttcc gtcaatgcgt cctccacgaa
     7441 cgcgccgtgc tgcttcgctt cggcctggct gacctcggcc catatccgtt tcacctgggc
     7501 cgcactgtac ccggccagct cggcggtctt gttgatgctg tagccggact tgcgcagcgc
     7561 gacaacttgg gcgcggcgct tcggatcagc acggcggccc ttgtaccgcc cggcctggcg
     7621 ggccaactca atgccttggc gctggcgttc gcgcctgtcc tcgtagtcgt cgcgggccat
     7681 ctgcaaggcc aggcgaaaaa gcatgatctg cacggcttcc agcacgatct tggcgacgcc
     7741 ctgggcctcg gccgccaggt cggatagatc gaccacgcca gggacggcca ggcgtgcgcc
     7801 tttggcctgt atcgaggcca ccaggcgctc ggcctcgggc aaaggtaggc ggctgatgcg
     7861 gtcgatcttc tcggcaatga ccacctcgcc gggctgtagg tcgccgatca tgcgcagcag
     7921 ctcaggccgg tcggcgcgtg cgccggatgc cttctcacgg tagatgccgg cgacgtagta
     7981 gccggcggcc ttcgcggccg tagtgatcgc ctcttggcgt tccaagtcct gcgcgtcggt
     8041 gctgacgcgc agatagaccc gcgccaccat cggcgcggct actggcttcg tcctgcgcat
     8101 acttgcgggc tcctttgggc atacgcaaag tgtccggtca tgctaccccc ggccaaaaag
     8161 gccgtcatgc gatttatctt ggccgggtcg ttattggcgg gtctaaatgg ccgctccgtg
     8221 gatctcatcg ccagaaatgg cgatgcgaga tccagggcag ggcggccgcc atcgtcagac
     8281 ctggcgatgg tcgacgccgg cgcgcatcgt cggatgtggc gatgcgagct cgaggtcggt
     8341 ggccaccgcc ggcgacctgg tgccatcgtc ggcaatggcg atgctgcagc tcgacgtgcg
     8401 cggcctggtc gtctcggcat cgtcggatgc ggcgatgctc gagcgaggcg aaaaaaaacc
     8461 gcctcggggt gtccgaggcg gcaagtctgc atgcggttca gggaggaaga aaccgcgccg
     8521 gcgatcaggg aggaaaaccg cgcggcctga tgtgcagctc ggccacttta gccggatttt
     8581 tcgacttatc cgatgcgagc ggcccgccgt gggcgctgct cgatctgctg ttgctttttc
     8641 tcgacgtgct tctgctcctg gtgctgctgc tcgtcgc
//
LOCUS       DACAPB010000183         8557 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.183, whole genome shotgun sequence.
ACCESSION   DACAPB010000183 DACAPB010000000
VERSION     DACAPB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.183"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            799..1017
                     /locus_tag="IT904_003882"
     CDS             799..1017
                     /locus_tag="IT904_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAP3193594.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1235..1477
                     /locus_tag="IT904_003883"
     CDS             1235..1477
                     /locus_tag="IT904_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193595.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1807..2736)
                     /gene="fdhE"
                     /locus_tag="IT904_003884"
     CDS             complement(1807..2736)
                     /gene="fdhE"
                     /locus_tag="IT904_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAP3193596.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2733..3368)
                     /gene="fdoI"
                     /locus_tag="IT904_003885"
     CDS             complement(2733..3368)
                     /gene="fdoI"
                     /locus_tag="IT904_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP3193597.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(3365..4267)
                     /gene="fdxH"
                     /locus_tag="IT904_003886"
     CDS             complement(3365..4267)
                     /gene="fdxH"
                     /locus_tag="IT904_003886"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP3193598.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(4280..7330)
                     /gene="fdnG"
                     /locus_tag="IT904_003887"
     CDS             complement(4280..7330)
                     /gene="fdnG"
                     /locus_tag="IT904_003887"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6743..6745),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP3193599.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7524..8357
                     /gene="fdhD"
                     /locus_tag="IT904_003888"
     CDS             7524..8357
                     /gene="fdhD"
                     /locus_tag="IT904_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAP3193600.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2033 a         2130 c         2194 g         2200 t
ORIGIN      
        1 tggctatttc ggttatatta aataagaact ctataaattt ttattcttcg ttggctccat
       61 acgtgtcttg ctacatcact gttcaggagc atttaaatat tatttgtaga actgagtggc
      121 aattatcgca gataatgctt aattcatcat cttagttcat gtttgagcga tttttgatag
      181 tcaaaacccc ctctggtgcc atacttttct cataaatatt tttaaaatct atagctaaca
      241 aatattatgc aaagataatg gactagcatc attgtcagta tcattcattt actgctctgt
      301 atgttatgaa taattatgtt gtatgtttgc tcttaaaccg tgggaaggag gctataatgg
      361 ttgccaatat taatctcatt aaacaagaaa gttattctgt tgttaattta gaaaaacaac
      421 tttcccgaac tgttaccaac aaaattataa cgatgagcaa gtaaagaccg gttggtggca
      481 gtactgaccg gtctgtgatg aaagattaat ttcaccttaa atcctggtgc tgttaggatg
      541 ctgacgtttt tcggctacac cattcagatt atgcaaataa atatctctga atataatata
      601 ttcatagcac ccataattca tcctgggcga taactaaacc atttttgagt aataaatgaa
      661 acaactgcaa cactcttatt atttctggaa aacgcctcgc aaaaacaaaa caacatgcaa
      721 aaatccgctt tttttaaatg attttccggc gtaattttcg cacatacatt aatgagtaat
      781 atatgtaagg tattggtaat ggcgatgaat acggtttttc ttcatttatc agaagaggca
      841 attaaacggc tgaacaagct tcggggctgg cgtaaggttt cacgatctgc aattttacgc
      901 gaagcggtag agcaatatct ggaacgtcag caattcccgg tgcgtaaagc aaagggaggc
      961 aggcaaaagg gggaggtagt tggtgttgat gatcagtgta aggagcataa ggaatgacaa
     1021 aaatttttca ttcttgaata taaaaaacag atgcccttat tctggtatta atacaaggct
     1081 gttttacttg aacttataat aactgcaact gttacatcat atctggaaaa cgcctcgcaa
     1141 aaataaaaaa tgatgcgtaa atgagctttc tttaaagatt cggcaagggt acttttctct
     1201 ccatagattc aaaaatgata ttggcgagat atttatgaac tctctggcag gtatagatat
     1261 gggcagaatt ttactcgatt tatcgaatga ggtgattaag caacttgatg atctcgaggt
     1321 gcagcgtaat cttcctcgtg cggacctatt aagggaagcg gtagatcaat acctgataaa
     1381 tcaatcgcaa acagcaagaa ccagtgttcc tggcatctgg caagggtgtg aggaagatgg
     1441 tgtcgaatat cagcgtaagc tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag
     1501 attgctgaca acgtgcgcgt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta
     1561 caaagtcgta taaattcaat gtattgcagt ttattggtag gcctgataag cgcagcgcat
     1621 caggcaatct tacgcttgtc atcagtctca tgcccataac cttattgatg tggtggtggg
     1681 taaattgagg ccaaaccggc actggcggca tagcttgagg tggtaacgcg gaatacgaga
     1741 gatggtgtcg tcacacccta tgagctgtaa tccgggcgac ctgcgccacc cggaaaacgt
     1801 cagagattac tctccttcac ccggaaacag gaacgggttg atggaactgc gggcatagcc
     1861 ttcttgctcc attcgtgcgt ccagtaccag agaggcgagg tcatctgcta cggcttcgat
     1921 ttttggatct ttttcctgat agagaatttt caggtaagtg ccgcagtcat cgcagctttc
     1981 ggctttaatc gcggcctgtt cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc
     2041 gcagttgctg cattttacgc gcactacgtg ccattcagtt tcacacaggt tgcagtgcag
     2101 gtaacgtagc ccctgagtgg tgccaatttg caccatgctg gacaccggca tagaaccaca
     2161 aaccgggcaa tattgacgtt gctcgccgta ttcagcgcgg gctttgccgg ggatcagatt
     2221 ggccatctgc gcccagtaga gcgatagtgc agcccagata aacggcgctt tatcgctgct
     2281 gacggacgag aaatcagagg caaacagcgc gctggccata tcttccagct cctgagtcga
     2341 tgccttctcc agattctcaa tcactgccag cgccgggccg ctcatttcag gtttcagctc
     2401 agcaatcagc gccatcagca gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc
     2461 cagcgggggc ttgccttgtg cgctggcttc tttaatgcgc gcagtcagat ccatctccag
     2521 cggatggtcg tacagcacca cttcctgggc gtgggcgata agcgcagcaa agcgcaggta
     2581 atcacccaga ggattatttt ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa
     2641 attcttgagc cgagggaaca ataacggcgg aatcatatcc gccgtacgtt tctcgcttga
     2701 acccagctca tcttgcggga ttatgcgaat actcattcag ctttcttttc cgttgtcttg
     2761 cggacctcac ggtaccagcg cgggtgatgt ttcttcgccc aggcgctggt aacccatcct
     2821 tccaccatgg cggtaatcgt gcctttcacc caaagggcgg cgtagatatg caccatgata
     2881 accacaatta acgccactgc ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg
     2941 attgagaaag caggcgcaaa ataaggacgc cagataatca cgccgctcac cagcagcaga
     3001 accaggaaaa taatcgccgc ccagaaaacg catttctgac cgaagttata acgcccggtg
     3061 tcacctactt cctcgttgac gacgatctta cgaatattct tcgcccaaaa gatatcatcc
     3121 cgattgatta ggttgtgatg ccagtaacgg aaaaacatga tgatgaacga ggcaaacata
     3181 accacgccga caaacgggtg cagaattcgc gccagctgcg gtgtgcccat gatttgcatc
     3241 aaccagttga aggacgggaa caaaaagccc agcccgctca ccgccgccag gatgaagcag
     3301 aaggcggtga tccagtggtt gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt
     3361 cgtttcattt gcgctcctcg tctttctctt cgtgcagatt attctcttcc tcatccgcac
     3421 ggttcggacc gacacccacg tagtggaaga tactggccgc aaaggtagcc gcaaagccaa
     3481 cagccgcgag cggtttccag atgcctttcc agaatttcac ggtttcgctg atttccgggt
     3541 tctccggcaa gccatgatac agatttggct tgtcagcatg gtgcagtacg tacatgacgt
     3601 gtgtaccacc gacgcctgcc ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca
     3661 gctcagcaac gcgctcgctc gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg
     3721 cgcccgttgg gcaggtcttc acgcaggctg gttcttgccc aaccaccaca cggtcaacgc
     3781 acagcgtaca tttgtagacg cggttgtctt ccgggttgag gcgcggaatg tcgaacggac
     3841 agcccgcaat gcaataaccg cagccaatgc actgctcgga ctggaagtcg acgataccgt
     3901 tggcatactg aataattgca ccttccgccg ggcacgcttt caggcagcct ggatcggaac
     3961 agtgcataca gccgtctttg cggatcagcc attccagttt gtcgttctgc tccacttccg
     4021 agaagcgcat taccgtccac gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa
     4081 tgttattgcc gacggtatcg cgaatgtcgt tccactctga acacgccacc tgacaggctt
     4141 tacagccgat acaggtggta acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg
     4201 cctgaggcgc gggggtcaga ccgttagtcg cggaacgacg aatgatatct tgagattgat
     4261 aagccatatg tcgtctccgt tacacctttt ccacattcac aaggaaggac ttaaactccg
     4321 gcgtctgcgt gttcgcatca ccaacgaatg gcgttaaagt attggcaata aagccttttt
     4381 tagctacgcc ttcatagccc cagtgaatcg gaataccgat ggtatcgata tctttgccgt
     4441 tcgctttcag cgtgcgaata cgtttggtca ccaccgcttt ggctttgata tagccacggt
     4501 tggaggagac tttcacggta tcgccctggg caatgccaag tttattcgcc agcgactccc
     4561 cgatttccac aaactgctct ggctgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt
     4621 agtggaagtg ctcggtcaga cgataggtgg ttccgacata cgggaactta tcggctttac
     4681 ccaatgcttc ggcgtcgtct ttaaagatac gtgcagccgg gttcgagata acgtttggat
     4741 gcagcgggtt agttcccagc ggcgtttcaa acggctcgta gtgttccggg aacggacctt
     4801 ccgccatctt atcgagggca aacagacgcc ccatgccttc ctgctgcatg ataaacggcc
     4861 cgacgccgct acccggaggc gctgcgctgt agtccggaat atcccagccg gtccacttag
     4921 tgccgtccca tttcagcaac tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg
     4981 aggcgcggtt atacagaatg cggcggttaa gcggccatgc ccatgcccag cccagcgtgt
     5041 taccgaggcc agacggatcg gcgttatcac ggcgtgccat ctggttgcct tccggcgtcc
     5101 agctaccggc gaaaatccag cagccacagg aggttgtacc gtcatcgcgc aattgggcga
     5161 acgaactaag ttgttggcct ttcttgacga taaccgcacc ggttgccgga tcggtaatat
     5221 cggccagcgc cttaccgttg ctctccatcg ccacttcttc tgaagatggc tcatgcggaa
     5281 tggcgtagtt ccatgtcatg ttcagcacct ggtccgggtt cgcgccaccc tgttcggcat
     5341 acatcttgcg caagcgcagg aagataccgg agaggatctc gccgtcagtc agcgcaatcc
     5401 ccggggcgtc cgcacctttc cagtgccact gtaaccagcg acctgagtta acgatagaac
     5461 cgttctcttc cgcgaagcag gtcgatggca gacggaacac ttcggtctgg atcttcgacg
     5521 agtcaacttc gttcagctca ccgtggttct gccagaagtt agaggtttca gtgttcagtg
     5581 ggtcgatggt gacgaggaac ttcagtttcg acagacaacc gatcactttg tttttgttcg
     5641 ggaatgaggc aacagggtta aagccctggc agatatagcc attgaccttg ccctctttca
     5701 tcatctcgaa gtattgcagg acgtcgtagc ctttatccca cttcggcaac cagtcaaagc
     5761 cccagctatt ttccgccgtc gctttatcac caaagaaggc cttcatcata gagacgaaga
     5821 atttcgggta gttgccccag tagtttacct ggccttccag cagcggtttt ggcgtgttgg
     5881 cggtaaggta ggtttgcaga tcggtctgct tctcgcttgg cagcgtcatg taacctggca
     5941 ggctctgcga cagcagcccc aggtccgtca gcccctgaat attggagtga ccgcgcaggg
     6001 cgttaacgcc gccgcctgcc atccccatat tgccgagcag cagctggatc atcgccatcg
     6061 tacgaatgtt ttgcgcacca acggagtgtt gcgtccagcc gagggcatac aggaacgagg
     6121 cagttttatc gtgagcactg gtttctgcga tgtattcgca gactttcagg aacgcgtctt
     6181 ttggcgtacc acagatgttt tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca
     6241 gcaagttcca cacgcagcgc ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt
     6301 cgtccagttc ataagtccag gaggatttat cgtacttgcg tttttccgcg tcgtagccgg
     6361 tgaacaggcc atcttcaaag ccgtaatcct cacgcacgat caggctggcg ttggtatagg
     6421 cttcggtgta ttcgcggttg aatttttcat tgttcagcag gtacagcaat acgcctgaca
     6481 ggaaagcaat gtcagtaccg gaacgaatag gggcatagta gtcagctacc gccgccgtac
     6541 gcgtaaagcg aggatcgatc acaatcagct tcgcgccgtt gtgaattttg gcttccatcg
     6601 cccagcggaa cccgaccggg tgagcttcag cggcgttacc gcccatcacc acgacgaggt
     6661 tggcgttctt gatgtcgacc cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa
     6721 gacttgctac cgttggtccg tgtcagacac gcgcctggtt gtcgaccgcg agcataccca
     6781 gcgcgcggga gaatttttgc gttaaatagc cggtttcgtt actcgacgcg gaagcacaca
     6841 gcatcccggt ggagagccag cggttaacag tcacgccttc ggcgttttgc gcaatgtagt
     6901 tagcatcgcg gtcttctttc atcagtttgg cgatgcgatc aaacgcctct tcccaactga
     6961 tttgctgcca tttatcagaa cctggcgcac ggtattccgg aaacttcaga cggctttcgg
     7021 agtggatgaa atccaccagg ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt
     7081 gatccggatc gccttcgata tggaagatag atgctttggc gttttttgct ccgtcaccga
     7141 ggctgtacat caacagccca cagcctacgg aacaataggt gcaggtatta cgggtttcgc
     7201 gggtgcgcag cagtttatac tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca
     7261 gtgccgctgc cgtggtgcct gccataccgc cagcgcagat cttaaagaac tgccttctgc
     7321 tgacctgcat ggattgctcc ttgtttcgac attgtcacgt cccatttaca ttcgcttgct
     7381 gcgtgtgcag ggagtgggag ttatttttct ttgcggaagg ggccgcaaag gtccagaatt
     7441 ggctcaattt ccctccatcc aggaagggtt tgtaacagaa taccataatg ttggtgtgtg
     7501 tgttcttatc tggttaagag aaagtgaaaa aaacacagcg aaaagaaatc gaaaatgtga
     7561 caaatatcac aggtgttcgt caaattgagt tatggcggcg tgatgattta caacacccac
     7621 ggttagatga ggtcgcggaa gaagttcccg ttgcgctggt ctacaacggc atttcgcatg
     7681 tggtgatgat ggcgtcgccc aaagaccttg agtactttgc gctcggtttt tcgctttccg
     7741 aggggattat cgaaagtccg cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg
     7801 gtcttgaagt acaaattgag ctttccagcc gccgctttat ggggttgaag gagcgccgcc
     7861 gggcgctggc gggacgtacg ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg
     7921 gaaaaccggt gcagccgcta ccgttcaccc agacgtttga tctcaacaaa ctggatgatg
     7981 cattacgtca tctcaacgat tttcagccag tggggcaact gactggttgt actcacgccg
     8041 ctgcctggat gttgccatct ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg
     8101 tggcgctgga caaactgtta ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg
     8161 cggtactggt ttccagtcgt gccagttatg aaatggtgca aaagtcggcg atgtgcggcg
     8221 tagagatttt gtttgcggtg tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct
     8281 gtaatctgac actggtaggt ttttgtaaac cgggtcgggc aacggtttat acccatccgc
     8341 agcgtttgag caattaatta aaataagtcc tgcgaaatat attaaattgc aggacttatt
     8401 catttcgtga attttattat tttatttata aacaaaatgc gtgtagtggc gttttctttt
     8461 ttcatttatt atgttcactc tcttatctat atatttataa ggcaattaaa tgaaaaggaa
     8521 tttattatct tccgctatta tagtcgccat catgtcc
//
LOCUS       DACAPB010000184         8453 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.184, whole genome shotgun sequence.
ACCESSION   DACAPB010000184 DACAPB010000000
VERSION     DACAPB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.184"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..256
                     /locus_tag="IT904_003889"
     CDS             <1..256
                     /locus_tag="IT904_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193601.1"
                     /translation="IIGLDGSVDRKETHRAPYVKGTFKVPKNFPVSKITSSDEMTITA
                     ELANGQVYVLSSAWLHGEANHNAEEGTVDLEFHGEEGDYQ"
     gene            256..525
                     /locus_tag="IT904_003890"
     CDS             256..525
                     /locus_tag="IT904_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP3193602.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            667..2502
                     /locus_tag="IT904_003891"
     CDS             667..2502
                     /locus_tag="IT904_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP3193603.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFKQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAQNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            2563..3891
                     /locus_tag="IT904_003892"
     CDS             2563..3891
                     /locus_tag="IT904_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAP3193604.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYISDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            3888..4967
                     /locus_tag="IT904_003893"
     CDS             3888..4967
                     /locus_tag="IT904_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAP3193605.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            4967..5515
                     /locus_tag="IT904_003894"
     CDS             4967..5515
                     /locus_tag="IT904_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAP3193606.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPRARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            5515..5940
                     /locus_tag="IT904_003895"
     CDS             5515..5940
                     /locus_tag="IT904_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193607.1"
                     /translation="MELWLTVNGKRTCASAELDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            5927..6985
                     /locus_tag="IT904_003896"
     CDS             5927..6985
                     /locus_tag="IT904_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAP3193608.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFTRLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIVPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            6976..7560
                     /locus_tag="IT904_003897"
     CDS             6976..7560
                     /locus_tag="IT904_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAP3193609.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRAHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVIECVLNKLCPSHTYVIFKYPE"
     gene            7564..7866
                     /locus_tag="IT904_003898"
                     /pseudo
     CDS             7564..7866
                     /locus_tag="IT904_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
BASE COUNT         1937 a         2090 c         2625 g         1801 t
ORIGIN      
        1 catcatcggc ctggacggtt cagtggaccg caaggaaact caccgtgcgc cttatgtcaa
       61 agggaccttc aaggtgccga agaattttcc ggtgagcaaa atcacctcgt ctgatgagat
      121 gaccatcact gccgagctgg caaacggtca ggtctatgta ctgtcgtctg cctggctgca
      181 cggcgaagcg aaccataatg ccgaagaagg cacggtcgat cttgagttcc acggtgaaga
      241 aggggattac cagtaatgaa agagcttgag ttaaagaaac cgattaccgc tcatggcgag
      301 acactctccg tactggagtt tgatgagccc accgggaaag atgtccgcga gctgggatat
      361 ccctaccaga tgaatcagga tgagtccgtc agacttctgg cgcatgtggt atcgaaatac
      421 atcgtgcggc tggcgaaagt gccgcaaagc tctgtcgacc agatgtctcc ggcagacctg
      481 aatgcagcgg cgtggcttgt ggccggtttt ttcctccagg cctgacggct gaatacctca
      541 ctgatcgctt ctttgactgc gccagttact ggcgcattaa tcccttcgaa ttgctgaata
      601 tgccgatcag tgaaattccc ttgctggtca gtcaggcaaa caggatagag caggagaaac
      661 gcacacatgg ctgaatttga gcttaaggcg ttgatcaccg gtgtcgacag gctttctccc
      721 gcgctgtcga aaatgcaaaa gaaaatccgg ggatttaaac gccaggcgga agaagcgtca
      781 cagggtgggc tggcgcttgg tggcggactg gcagcgggtc tgacgctttc cctgaaatct
      841 tatgccgatc aggaaaacgc cgccaccggg ctgaaagtcg ccatgatgga tgcgaacggc
      901 gaggttggaa agagctttca ggacatcaat aaactggcta ttggcctggg taaccagcta
      961 cccggtacaa cggctgattt ccagaacatg atgcagatgc tggtgcgtca ggggatcccg
     1021 gcagaaaaca ttcttggtgg tgtgggtaaa gcgacagctt atcttgcggt acaactgaaa
     1081 aaaacaccgg aagcggctgc tgagtttgct gcaaagatgc aggatgctac cggaacggcg
     1141 tcagaagaca tgatggggct gttcgacact atccagaagg cgttttatct gggcgttgac
     1201 gataccaaca tgttgtcctt cttcactaaa accagttctg ttctgaagat ggtgaataag
     1261 gacggacttc aggctgcaca gagccttgcc cctatcagcg tcatgatgga tcagatgggg
     1321 atgaacgggg agtcggcagg taatgccctg cgaaaagtta tccagtccgg attaagcgtt
     1381 aagaaaatca gggacgtcaa taaagtcatg gcccgccaga aactcggagt gcagctcgat
     1441 tttactgatg gcaaagggag ttttggcggt cttgataaca tgtttaagca actggcaaag
     1501 ctgcgaaaac tgaccgacgt taagcgaaca ggtgtactta aggcaatatt tggtgatgat
     1561 gccgaaaccc ttcaggtggt caatgctctg atcgataaag gaaaggatgg ttacgatcag
     1621 atccagcaga agatgaataa acaggccagc ctgaataaac gtgttcaggc acagcttggt
     1681 acgctgtcca acctgtggga ggcaatgacg gggaccgcaa ctaacggtct tgcagctatt
     1741 ggcggcgcat tttctggtga cgcccaaaat atcacgcaat ggctggggga gttaggggaa
     1801 aaattcacga agtttgcgga tgaaaatccc cgggttattc gcggcgtcgt cgggcttgct
     1861 gccggtcttg cgattctgaa actgggattg atgggcgttg gcggtgccat cagtattgtc
     1921 agcaggatca tgtcgatgac gccgattggc atgattgcga cggcgatagc cctggctgcg
     1981 ggattaatta tcactaactg ggatgttgtc ggaccttatt tcaagaagct ctgggaaacc
     2041 attggtcctt attttgaggc tggctgggaa cttctgaaga aggtttttgc ctggtcgccg
     2101 ctggggatgg taatcaataa ctggggaccg gttgttaagt ggtttcagga tatgtgggac
     2161 aagctgaagc caattattga gtggtttacc gacagttccg gtgacacggt cgatgccatt
     2221 aactctgcgc agtggggcgc gggtgcttat gatgcttatg ggacgggaat accggcacgg
     2281 ggatacacac cttatcaggc ggtggatccg gctcagtcaa acaacgcctc cgatgccaca
     2341 ggcccgaatc ccttcatgat taacaaagct tctgcgccaa aagttgatgg tgagatcaag
     2401 gtctcttttg tgaattcgcc tccgggtatg cgggttatgg aaacgcgatc cagcggtttt
     2461 gatgtcagcc atgatgttgg ctatacgcgc tttggcaggt aatgaaaaat taatctgtta
     2521 atgagtccca ctccggtggg attttttatg tacggagttt atatgacgtg gaaagacaga
     2581 cttcaggacg cgtcatttcg cggtgtgcca tttaaggttg aagaagaaag tgcgggaacc
     2641 ggtcgtcgtg tggaaacgca cgaatacccg aaccgcgaca aaccctatac cgaagacctg
     2701 gggaaaatca ctttccgccc gtccatcaca gcttatgtgg tgggagatga ctgctttgac
     2761 cagcgcgatc gcctgattga cgcgctgaat aaacccggtc ccggcacgct tgtccatccg
     2821 acttacggtg agctgaaagt ctgtgttgac ggggaagttc gggtcagcac atcgaagagt
     2881 gaagggcgta ttgtccgctt tgacctgaag tttgtcgaag cgggagaact ctcttacccc
     2941 acttcaggtg cggcgacggc gcagacgctg atgtcatcct gttctgcact ggatgactgc
     3001 atcagtgaca gcttcagtgg tttcagtatc gatggcgtgg cagattttgt gcagaacgac
     3061 gtcgtcggta atgccagcac aatgcttggg tatatttctg atgcgatgaa agtggtggat
     3121 tctgccgtat cggatgccgc caggctgttg cagggggata tctcggtact tctgccgccg
     3181 ccatcgtcag gaaaaaattt cgttgagcag gtgcagaaaa tgtggcgtac cgggaaacgc
     3241 ctttatggta acgccagcga cctggtcacc atgatcaaaa cgctttccgg tgtcagcctc
     3301 ggcagcgatc tgcaaccgcg cggcgtctgg aaaacggaca gtaaaaccac cgccacggcg
     3361 acgcagcagc gtaacgtggt tgccagcacc cttcgtacga ccgcaatcag cgaagcggcg
     3421 tatgccgtca cacgattgcc tgcgcccaca acttccgcgg tgatgcagaa tgccacagtg
     3481 gggcagtcaa caacacccgc gcagagcacc ggctggcctt ctgtcacgca tccggcactg
     3541 aacaatgcac cggcggtgaa aaacacggtt gacctgccaa cgtgggaaga actgaccgac
     3601 attcgcgaca cactgaatac ggcaattgat aaggagttgt cccgtacaac cagtgatgcg
     3661 ctgtttctgg cgctgcgccg ggtgaaagca gatctgaatg cggatatcaa cacgcgcctt
     3721 gaacagtctg cacggatcat tcagcgcacg ccggatgagg ttttacccgc gctggtgctg
     3781 gcggcgacct ggtttgataa cgcggcgcgt gacgcggaca ttatccggcg taatgccatt
     3841 acgcatcccg gctttgtgcc ggtgatccct ctgaaggtgc cagtgcaatg aacgacaatg
     3901 tcacgctacg ggtaaatggc cgggagtgga atggctggac atcggtgcgc atcggtgccg
     3961 gtattgaacg gctggcgcgg gatttcagtg tggagatcac tcgccagtgg ccgggagatg
     4021 agggtatcac cacgcttcag ccgcgcatta aaaacggttc aaaagtggaa gtgctgattg
     4081 gtgatgagct ggtgatcacc ggctgggtgg aggcgactcc cgttcgttac gatgcccgtt
     4141 cggtcagcac cggtattgcc ggacgtagtc tgacggctga cctgattgac tgtgcagccg
     4201 aaccgacaca gtttaacgga cgctcgctgg tgcagattgc gcaggcgctt gctgcgcctt
     4261 tcggcattga ggtggtgaac agcggtgcgc cgtcgggtgt tattcctgat gttcagcctg
     4321 atcacggtga aacggtgatt gaggtaatca acaaaatact cggtcagcag caggcactgg
     4381 cttacgacga cccgcacggc aggctggtga ttggcggtat tggctcaacg cgggcacata
     4441 ctgcgctggt actcggggaa aacatccttt cctgcgatac ggagaagagt atccgggagc
     4501 gattttctgt ttaccaggtg gcggggcagc gtgccggaaa cgacgatgat ttcggtgagg
     4561 ccaccaccac cgcgctgcgg gcccgcacag aggacgcatt tattgcccgt taccgtccga
     4621 tgtatatcag gcagacaggg caggctacgg gggcaggctg tattgcccgt gcggactttg
     4681 aagcccgaca acgggcggcg cggacggatg aaaccaccta tgtggtgcag ggctggcgac
     4741 agggtaacgg tacgctgtgg cagcccaacc agcgggtgat tgtctttgat ccggtctgtg
     4801 gtttcgacaa taccgaactg cttgtttcgg aagtcacgtt tactcaggac cagaacggca
     4861 ccctgacgga aatccgtgtc ggcccacctg atgcttatct gcctgaaccc gaagatcccg
     4921 gtgcgcggaa aaagaaaaaa gccagagtac aggaggaccc gttctgatga ggacgattga
     4981 agccatgcag cgacaacttc tcggcctgat tgggcgggca gtggtgaaaa gcatcagtgc
     5041 cgccacgaaa tgtcagaccg tggatgtgtc cctgattgcc ggtgaaccca aagccggggt
     5101 tgaacatctt gaaccctacg gttttaccgc aagggcaaac agcggtgcgg aagcggtggt
     5161 gttgtttccg gatggcgacc gttctcatgc ggtggttgtt acggtgtcgg accggcgcta
     5221 ccgcctgaaa gggctgcaga cgggtgaggt ggctgtctat gacgatcagg ggcagtctgt
     5281 gacgctgacc cgggagggga tcgtggtgga cggtgcaggt aaaacgatca cgtttcgcaa
     5341 tgcgcccaga gcacgttttg aaatggacct ggaagtgacc ggacaggtga aagacctgtg
     5401 cgactccggc ggtaccacca tgtcagcgat gcggcttgcc tataacgggc atcgtcacag
     5461 agagaacggt cagggcagta acaccgacaa acctgataaa gcgatggagg catgatggaa
     5521 ctgtggctga cggtgaacgg taaacgcacc tgcgccagcg cagaactgga tccgctgacc
     5581 cgcgccgtgg tgatttccct gtttacctgg cggcgggcgg agcctgatga caacgccgac
     5641 gtcccgatgg gatggtgggg ggatacctgg cctgcggtac agaatgaccg ttacggctcc
     5701 cgactgtggc tgcttcagcg cagcaaactg accaatcagc tggtgcagac ggtaaggggg
     5761 tatatccgcg aatgcctgca atggatgatt gatgacggcg tggtgtcccg tattgatctg
     5821 gatatccgcc gcaccgggat taatgaactg ggtaacagta tcactctctg gcgtcgtgac
     5881 ggaccggtaa tgatttcttt tgatgatctg tggagtgcga taacgcatgg cggacagtga
     5941 atttcagcgc ccgacgctgg cagaaaatat cagtatgctc cgtaacgatt tattcaccag
     6001 gctggacgtc agcgacacgc tccggcgcat ggatgaagac gtgcgggcaa aggtgtatgc
     6061 ggcggcgctg catacggttt acggttacat cgattatctg gcaatgaaca tgctgcctga
     6121 cctgtgcgat gagtcctggc tggcgcgaca tgctgcgatg aaacggtgtc cgcgcaaggg
     6181 ggccacggct gccagcgggt atatgcgctg ggaaggtgtc agcgatggcc tgaaggtgac
     6241 cgccgggagt gttattcagc gcgatgacct ggttcagtac acggcaactg ccgatgcaac
     6301 cagctccggt ggtgtcctgc gcgtgccgat cgcctgctca agtgcaggtg cggtcggtaa
     6361 cgctgacgac ggtacggcat taatcctggt cacgccggtg aatggtctgc cgtcttccgg
     6421 tgtggctgac acccttacag gcggatttga tactgaagag ctggaaacgt ggcgcgcccg
     6481 cgtcattgag cggtattact ggacgcctca gggcggggct gacggggact atgtcgtctg
     6541 ggctaaagaa gtgcccggca ttacccgcgc atggacatac cgtcactgga tgggaacggg
     6601 aactgtcggt gtgatgattg ccagcagtga cctgattaat cccattccgg aagaatcaac
     6661 ggaaacggca gcaagacaac atatcgtgcc actggccccg gtggcaggct ctgatttgta
     6721 tgtattcagg ccggtggcgc acaaagtgga ttttcatatc cgcgtgacgc cggacacacc
     6781 agaaatacga gccgccatca ccgcggagtt gcgttcgttt cttctgcgtg atggttatcc
     6841 gcagggagaa ctgaaggtat cgcgtatcag tgaggcgatt tccggtgcga acggggaata
     6901 cagccatcag ttgcttgcac cggcagacaa tatctccatt gcaaaaaatg aactggcggt
     6961 actggggacg atttcatgga cgtgacaaac gatgattaca tccgtctgtt gtcggcactg
     7021 ttgccccccg gtccggcgtg gtcagccagc gatccggcga ttgccggtgc ggcaccgtca
     7081 ttaacccgtg ctcatcagcg tgcggatgcc ctgatgcggg agctggatcc gcgcaccacc
     7141 accgaactga taaaccgctg ggagcgtctg tgcggcctgc cggatgaatg tattccggca
     7201 gggacgcaga cccttcgcca gcgtcagcaa cggctggatg cgaaggttaa cctggcgggt
     7261 ggcatcaatg aggattttta ccttgcgcag cttgctgccc tgggcagacc agatgccacc
     7321 atcacgcgat acgacaaaag cacgttcacc tgctcatcgg cctgtactga cgcggtgaat
     7381 gcgccggaat ggcggtatta ctggcaggtc aatatgccag ctgccaccaa caccacctgg
     7441 atgacatgtg gcgatccctg cgattccgca ctgcgtatct ggggggacac cgttatcgag
     7501 tgcgtgctta acaaactctg cccgtcgcat acctacgtaa tttttaaata tccggagtaa
     7561 tccatgcatc gtatagacac gaaaaccgcg cagaaggata agttcggcgc gggtaagaac
     7621 ggttttaccc gtggtaaccc ccagaccggc acgcctgcca ccgatctgga tgatgactac
     7681 tttgacatgt tgcaggagga gctctgcagc gtggtggagg catccggtgc cagcctggag
     7741 aaggggcggc atgaccagct gcttaccgcg cttcgtgcgc tgctgttaag tcgcaaaaat
     7801 ccgtttggcg atatcaaatc ggacggcacg gtgaaaacgg ctcttcaaaa ccttggtttg
     7861 ggagatacaa gcggatacgt gggacgccgg ctgagtaccc gggctttcgc gtcatcaggt
     7921 acgtataccc catcaccaga aacaaaacgg atcagggtca caataacggg cggcggtggc
     7981 ggagggggtg gctgccaggc tatatccagt aacgaaacgt ttttcggtgc tggcggtggg
     8041 gccggaggga cagtaatcac catactgacc ccgacacaga atagttatcc tgtcactatc
     8101 ggcgcaggtg gggccggcgg cgttggtgcg acgaacggcc tcaagggcgg tgatagctcg
     8161 ttcggatcgg taatagcccc tggtggtgaa ggcggcggaa aagtaggagt cacaaacaca
     8221 aacggcggta acggaggcgt accgaatatt ggcgatatcc gcatcactgg tggagatgga
     8281 ggcgacggtc agtccgggaa tatcagcgtc agcggtgaag gcggtgcatc gtactgggga
     8341 ggcggtggac gtgcaggcgc tggcggtggc gttagaggca gggcatttgg ttcaggcgga
     8401 ggtggtgcat acgatgcagg ttatagcgga accagtatga cgggcgggaa agg
//
LOCUS       DACAPB010000185         8426 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.185, whole genome shotgun sequence.
ACCESSION   DACAPB010000185 DACAPB010000000
VERSION     DACAPB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.185"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            474..1745
                     /locus_tag="IT904_003899"
     CDS             474..1745
                     /locus_tag="IT904_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP3193610.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1775..2779)
                     /gene="dppF"
                     /locus_tag="IT904_003900"
     CDS             complement(1775..2779)
                     /gene="dppF"
                     /locus_tag="IT904_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAP3193611.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2776..3759)
                     /gene="dppD"
                     /locus_tag="IT904_003901"
     CDS             complement(2776..3759)
                     /gene="dppD"
                     /locus_tag="IT904_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAP3193612.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDVGRPTL"
     gene            complement(3770..4672)
                     /gene="dppC"
                     /locus_tag="IT904_003902"
     CDS             complement(3770..4672)
                     /gene="dppC"
                     /locus_tag="IT904_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAP3193613.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4682..5701)
                     /gene="dppB"
                     /locus_tag="IT904_003903"
     CDS             complement(4682..5701)
                     /gene="dppB"
                     /locus_tag="IT904_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAP3193614.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6009..7616)
                     /gene="dppA"
                     /locus_tag="IT904_003904"
     CDS             complement(6009..7616)
                     /gene="dppA"
                     /locus_tag="IT904_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAP3193615.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8359..>8426)
                     /locus_tag="IT904_003905"
     ncRNA           complement(8359..>8426)
                     /ncRNA_class="other"
                     /locus_tag="IT904_003905"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         1929 a         2280 c         2146 g         2071 t
ORIGIN      
        1 agccgcctgt gttgtaatgc caacgtttcg cggctattct tgagttgttt gggttcaaga
       61 ttagcccccg tgatgttgtc aggtgcatac ctgcaacgtg cgggggtttt ctctctccag
      121 caaccaatgc caccagggat aaagcccccg caacattgcg cctcaccgga acctttccgg
      181 cttgccgctg attctacgat tgctttttac tgtcagcacc cggcaatctt cctcattgcg
      241 cgatgccttc cagcttaatt tctttatttg caatagttgc agttcatatc cgggctgact
      301 ccagactggt agtgaaatgc gcaattttgt agaattgcgc ctcctcgcga cctccccagt
      361 gaatctgttg cctcattttt tacatactgc gtattcgact tctccacctg ttgcgcaaga
      421 gaaactgggt ttattcattt ttgcgaggcc gacttctttc tggacaggac tttatgcagc
      481 acaacacact atcgaaacac aatcagaaat tgccgtttac acgctacgac ttcggctggg
      541 ttttattatg cataggcatg gcgattggtg ccggaaccgt gctgatgcca gtacaaattg
      601 gcttgaaggg aatttgggta tttattaccg cagcgatcat tgcttatcct gccacctggg
      661 tagtgcagga catttattta aaaacccttt ctgaaagcga ttcctgtaat gactacaccg
      721 atattatcag tcattacctg gggaagaact ggggaatttt cctcggggtt atctactttt
      781 tgatgattat ccacgggatt tttatctact ctctctccgt ggttttcgac agcgcctcgt
      841 acctgaaaac cttcggttta accgatgccg atctttcaca atctctactt tataaagtcg
      901 ctattttcgc cgtactggtg gcgattgcgt ctggtggtga acgattactg tttaagattt
      961 ccgggccaat ggtggtggtc aaagtaggga ttattgtcgt gttcggtttt gcgatgatcc
     1021 cgcactggaa tttcgccaat ataaccgcct tcccgcaagc ctccgtcttt ttccgcgatg
     1081 tcttgcttac cattccattt tgcttctttt ctgcagtatt tattcaggta cttaacccaa
     1141 tgaatattgc ctatcgtaaa cgggaagcgg ataaagtact ggcaacccgg ctcgcgctgc
     1201 gtacccaccg aattagttat atcacgctca tcgcggtgat cctgtttttt gccttttcgt
     1261 ttaccttctc aattagccac gaagaagccg tttctgcctt tgaacaaaat atctcagcac
     1321 tggcgctggc cgcgcaggtg atccctgggc atatcattca tatcacctct acggtgctta
     1381 atatctttgc cgtactgacc gcattctttg gcatttatct cggtttccac gaggccatta
     1441 aaggcattat tctcaatctg ttaagccgaa ttattgatac caagaaaatt aactcacgcg
     1501 tgctgactct ggcgatctgc gcttttatcg tcattacgtt gacgatttgg gtttcgtttc
     1561 gtgtatcggt gctggtgttc tttcagttgg gaagcccgtt atatggtatt gtgtcgtgcc
     1621 tcattccgtt tttcctgatc tataaagtcg cacaactgga aaaacttcgc ggatttaaag
     1681 cctggctgat tctgctgtac ggcattttgc tatgcttgtc gccactgttg aagctgattg
     1741 agtaaaccgg agcgcatggc cccggttttg tgagttaacg ctgcggattt tcatcctgat
     1801 caacagcaaa acaagctacc agttgaccgc cgtagtcttt tagctgcggc tgcaactggg
     1861 tgcaggggcc gaagcgccga cgacagcggg cgttgaaggc gcaacccggc ggtggattca
     1921 gtgggcttgg tagttcaccg ctgagcttga tgcgctcgcg gcgatcgtcc gggttcaggc
     1981 gcggcgtcgc ggaaagtagc gcctgagtgt acggatggcg cgggttattg aagatttggt
     2041 ctttcgttcc cttctccacg cagcggccca ggtacatcac catcacttca tcagcaatgt
     2101 gctccaccac cgacaggtcg tgggagataa agacataaga cagccccaac tcctgctgca
     2161 aatccatcat cagattcagc acctgcgcgc gcactgaaac atccagcgcg gaaaccggtt
     2221 catcggcaat caccacatcc gggtcgagca tcagaccacg ggcgatggcg atacgctgac
     2281 gctgaccgcc ggagaacata tgcggatagc ggtcatagtg ctcggttttc aggccgactt
     2341 tcgccatcat cgacagggct ttttcccgac gctgttcttt gcttaagctg gtgttgatca
     2401 gcagcggctc ttcaagaatt tgcccgactt ttttacgcgg attcagcgaa ccgtaagggt
     2461 tctggaagac gatctggatt ttctgccgac gcagcttctg cgcctgcgga tcgtgcttaa
     2521 gcagatcctg cccctggtaa tacagctcgc caccggtggg catttcaatc atcgtcagca
     2581 accgaccgag ggtcgattta ccgcagccag attcgcccac tactgccagc gttttgccac
     2641 gttcaaggtt aaacgaaacg ccatccagcg ctttaaccag acgttccggc gcgaacatgc
     2701 ctttcttcac cggataatgt tttttcaggt cgatagcctg caacagcggt tgttgcaggg
     2761 tggcctcttg cgtactcata gtgtcggcct cccgacatca tcaagtgggt aatggcattt
     2821 ggactgacgc ccgtcagcga gcatattcag cgccggttct tcagcgcgac atctgtcagt
     2881 ggcatagggg cagcgcgggt taagcaggca gccgttcggg cggtcgtact tgccgggaac
     2941 gacacctggc aacgacgcca gacgttcttt gtcctgagca aattctggca gcgcacgcag
     3001 caatgcctga gtatacgggt gacgcggcgc atggaagatg gcgtgcgcat caccggtttc
     3061 caccacctgg cctgcataca tcacgatgat tttatgtgcc gcttccgcca ccagcgccag
     3121 gtcatgggta attaacacca gcgccatgtt ctctttctgc tgtagctcca gcagtagttc
     3181 gatgatttgc gcctgaatgg tcacgtccag cgcggtggtc ggttcatcgg caatcagcag
     3241 ttttggccga caggcaatcg ccatggcgat catcacgcgc tggctcatgc cgccggaaag
     3301 ctgatgcggg taaacatcca gacgcgatgc cggatcggga ataccgacct gattcagcag
     3361 gtcaatcgct cgctgacggc gggtactttt gttgccgccc tgatgcacct taatcgcttc
     3421 cataatctgg aaacccacgg tgtagcacgg gttaaggctg gtcatcgggt cctggaagat
     3481 catcgccact tcggcaccca ccaggttgcg gcgctctttt tctgagatac gctgcaaatc
     3541 ctggccgtta aactccagtt tttctgccat tacgcggccc ggataatcaa tcagccccat
     3601 aatcgccagt gaactgaccg acttaccgga gccggactca cccacaatcc cgaccacttc
     3661 gccctgtttt acgctgtagc tgatgcggtc tacggcgcgg aacggcgcgc tttcgtcgcc
     3721 gaaatgcacc gataatttat ctacatttaa taacgccatc tcgaacctct tactgcttca
     3781 gtttgggatc gagcgcgtca cgcagaccgt cacccatcag gttaaatgcc agcaccgtca
     3841 gcaggatcgc cagacccggg aaggtcacga cccaccaggc gctttgcgcg aactgcaaca
     3901 cgtcggagag catggtgccc cactcaggcg ttggcggctg tgcccccatg ccgaggaaac
     3961 caagagcagc catatcgaga atggcgttag agaaaccgag cgacgcctga acaatcagcg
     4021 gcgcaaggca gttcgggaag atgttaataa acatctgacg catcgccccg gcacccgcca
     4081 cgcgagacgc ggtgacgtaa tcgcggttaa cttccaccag cacggcggcg cgggttaagc
     4141 gcacatagtg cggcaaggca acgaaggtca gtgccagcgc ggcgttacca atcgacgggc
     4201 cgaaaattgc caccagcacc agcgccagca gcagacttgg cagcgccagc atgatatcga
     4261 ccacgcgcat aatgatgtta tcgaccaggc cgccaaagta accggcgatc aggccgagaa
     4321 taacgcccat aatcagcgat aacacaacta ccagacagcc aaccagcagc gacaggcgcg
     4381 caccgtacat caggcgcgac agcacatcac ggcctacgtc atcggtgccc agcaagtgcg
     4441 ccatgctgcc gccttcctgc caggctggcg gggcgagcag tgcatcgcgg aactgttccg
     4501 ccgggttata gggtgcaatc cagttggcaa agatcgcgat gaacagcacg atgacgacgt
     4561 aaaccagccc gacgaccgcg cctttgttgc gtttaaaata gtgccagaac tcctgtaacg
     4621 gggtcatcgg caccggtgcg ctaatcactt tattttcagt aacctgtgac atgatgtccc
     4681 cttacttctt atgacgaata cgcgggttca ccacgccgta cagcagatcg accagcaagt
     4741 tgacgaggat aatcatcgtc gccaccagca atacgccgcc ctgcactacc ggatagtcgc
     4801 ggcgttgcag tgcgtcaatc aaccagcgtc ccagaccggg ccacgagaag atggtttcgg
     4861 tcagaatcgc ccccgccagc aatgttccca cctgcaggcc gataacggtc accaccggca
     4921 gcatcgcgtt acgcagcgca tggacgataa tcacccgcat gcgggttagc cctttggcgc
     4981 gcgcggtgcg gatgtaatcc tcgcccagca cttccagcat cgaggagcgt gtcatacgca
     5041 caatgaccgc cagcggaata gtacccagca caatggcagg caagatcata tgggcgacgg
     5101 catcgataaa gttgccgtct tcaccccaga tggcggtgtc gattagcata aaaccggtta
     5161 acggattgga gtcatcgagg aacaccatat cgctcacgcg accggagacg ggcgtcaggt
     5221 tccagtgcac cgaaaccagc atgatcagca tcatgcccca ccagaagata ggcattgaat
     5281 aacctgtcag cgccaggcca accgctgtgt gatcgaaaat ggaaccgcgt ttaaccgcag
     5341 ccagcacgcc gaccggaata ccgactgccg tagcaaaaat catcgcgcag acgccaagtt
     5401 ccagcgtggc ctggaagcgc ggcacgaact cttcccaaac cgggatgcgg cttttcattg
     5461 aaatgcctag atcgccatgc ataacgcccc aaatgtaatg gagatactgc tgccacatcg
     5521 gtttatctaa gccgagttca gccagcagct gcgcgtgacg ctctggggag atcccacgtt
     5581 cgcccgccat gatcatcacc ggatcgcccg ggatcatgtg gacaaaggca aatgtgagaa
     5641 gggtaatacc gataaacgtg gggatgacga gtcccaaacg tcggagaata aactgcaaca
     5701 taacccggat tctctatagt gacccgcagc ctggaactgc gtgtctgtat tgctcacaaa
     5761 tcaattcccg cccttatcgg acagggaatg aagtattgct gccggatggc gctgcacgca
     5821 gaggcacagc gccaccgggt atacagattg ttgacaaagt gcgctttgtt tatgccggat
     5881 gcggcgttaa cgccttatcc ggcctacaaa atcgtgcaaa ttcaatacat tgcagaattt
     5941 tcgtaggcct gataagcgga gcgcatcagg catttttgcc tttgccatca gtcttgtatg
     6001 gcttttaatt attcgataga gacgttttcg aagtgatgtt tgcctaatgg atcaaccaca
     6061 tagcctttaa cttctttacg taccggttca aacacggtgg agtgagcgat gatcagtgcc
     6121 ggagcctgat cgtgcatcac cacctgcgct tgtttgtaca gttcaacgcg tttattgtgg
     6181 tcgtcggtag cacgcgccgg ttgaatcaga tcttcaaacg gtttgtagca ccattttgag
     6241 tagttggagc cttgttcaga ggcggcgcag ctgaacaggg tggcgaagaa gttatccgga
     6301 tccccgttat cgccagtcca gcccatcatt accgtctggt gctcgccatc tttcgcacgc
     6361 ttgaggtact caccccattc gtaggtgaca attttggcct gcacgccgac tttcgcccag
     6421 tctgcctgaa tcatctccgc catgcggcga gcgttcgggt tatacggacg ttgtaccggc
     6481 atcgcccaca ggtcgatgga gaaacctttt tccagacccg cttctttcag caaggctttc
     6541 gctttttcag gatcgtaggt gtagtcctga acgtcgtcgt tatagcccca catggttggc
     6601 gggatcaggt ttttcgctga tacgcccgcg ccctgataaa ccgctttgat gatcgcgtct
     6661 ttgttcaccg cgtaggtcag agcctggcga actttcacgt catcgagtgg ttttttctgc
     6721 acgttatacg agagataacc gacgttcagc cccggcattt ccatcagatt gatggattta
     6781 tcctgcttca tgcgagcgat atctgccggg ttcgggtacg gcatcacctg gcattcattc
     6841 ttctgcaatt tcgcgtaacg cacggaagcg tcaggggtaa tagagaaaac cagcgtatcg
     6901 atctgcggtt tggtgcccca gtagccatca aacgctttgt agcggatacg ggaatctttt
     6961 tgatactgct gtaactggaa cggaccggtt ccgattgggt tgaggtccag tttttccggt
     7021 gtaccggctt tcatcatcgc atcagcatat tcttttgaca gaatagaggc gaagtccatt
     7081 gccaggtcag cgaggaacgg cgcttccggg cgagtcagca caaactgaac ggtgttgtcg
     7141 tccacctttt tcacttcact gatcagctct ggcaagccca tgccttcgaa gtattcgtag
     7201 ctgccgccag aaactttatg gtacgggttt tgcgcgtttt tctgacgatc gaacgagaac
     7261 accacatcat cggcgttcag ttcacgcgtc ggtttgaatt ctttattgtc gtgccacttc
     7321 acacctttac gcagatggaa ggtataggtt ttaccgtctt cgctgacttc ccacttttca
     7381 gcgaggcccg ggatcacttc ggtggtgccg attttaaatt caaccagacg gttataaagc
     7441 gggacggaag aggcgtcata ggtggtgccg gaggtaaaca gctgcgggtt aaacccttcc
     7501 ggagatcctt ctgagcaata aaccagagtt ttagcctgaa cacttgctgc gacggtcata
     7561 gccaccaggc tgagaccaag cttcagcatc cctgactttt tcaaggaaat acgcattatt
     7621 ctgctccaat tgtgatgttt gttgttttaa ccctttgcag tgggttgtcg ctgcctgacc
     7681 ttttttgttt tttgcccggt cgggtcaacg ttatgaggtg gggatgccgt actaattaac
     7741 atcagtgtaa cagtgcggat cctccataaa atgcccctcg tgacctacaa tctgtcaaca
     7801 gaatgtgaaa acgtcaatac agccaaccgg gatttacacc aacggtgaga atccacaaac
     7861 aaagattaaa aaaacttcaa acagctattt gcagcagaga aatttgtgct actccaacat
     7921 gaccagaaca atcagcttaa tatttagcaa catttggtga ttaagtttta tgcatttaat
     7981 gaaaaaaatc tgaggaaaag gtggatatct gagcgattaa taccatgaac gctaaaacgc
     8041 acagcggaaa atgccagggc cagccataag taacgcaaaa aaagatttaa gcaaatataa
     8101 aaaaagacaa tggtttctgt cacaaatccg ttaatggata gtgagatatg gggcgcaaat
     8161 ttggcaggga aatgtgtgtc gtgtaggtcg aataaggcgg tcgcgccgca tccgacaatg
     8221 gccggcacat ttgcctgatg cgacgctgac gcgtcttatc aggcctacgt cgagtcgaac
     8281 ctccctgccc ccggaggttc tcatcctcat gagctgcaga tgcaaaaaag cctgctcgtt
     8341 gagcaggctt ttcgaatttg gtcggtgata gaggattact cgccacttcg tggctcgccc
     8401 tgcgggccgt tgctggcgca acgttc
//
LOCUS       DACAPB010000186         8391 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.186, whole genome shotgun sequence.
ACCESSION   DACAPB010000186 DACAPB010000000
VERSION     DACAPB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.186"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..1519
                     /locus_tag="IT904_003906"
                     /pseudo
     CDS             1..1519
                     /locus_tag="IT904_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1488..3224)
                     /gene="aceK"
                     /locus_tag="IT904_003907"
     CDS             complement(1488..3224)
                     /gene="aceK"
                     /locus_tag="IT904_003907"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAP3193616.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3407..4711)
                     /gene="aceA"
                     /locus_tag="IT904_003908"
     CDS             complement(3407..4711)
                     /gene="aceA"
                     /locus_tag="IT904_003908"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAP3193617.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4741..6342)
                     /gene="aceB"
                     /locus_tag="IT904_003909"
     CDS             complement(4741..6342)
                     /gene="aceB"
                     /locus_tag="IT904_003909"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAP3193618.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6611..7540)
                     /gene="metA"
                     /locus_tag="IT904_003910"
     CDS             complement(6611..7540)
                     /gene="metA"
                     /locus_tag="IT904_003910"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAP3193619.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7697..8140
                     /locus_tag="IT904_003911"
     CDS             7697..8140
                     /locus_tag="IT904_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP3193620.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRVLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2152 a         2085 c         2065 g         2089 t
ORIGIN      
        1 agacaaatga acaccaaacc ctgagtgaat tgttttataa agaactcgag gataaatttt
       61 ctggcaagga gctggcgacg cctctattaa aaagcttctc agagaactgt agacaaaatg
      121 gtcgtcatat ctttagcaac aaggattttg tcattaaatt ttccacgtcc gtcttacaag
      181 ctgataagaa agaaattacg ataattaata aaaacgaaaa cacgacactt actcaaacca
      241 ttgccccaat atttgaaaaa tacctaatgg aaattttacc tcaacgctca gacactcttg
      301 ataaacaaga attaaaccta aagtcagata gaaaagaaaa agaattccca agaattaaac
      361 ttaatggtca atgttatttt ccggggcgac cccaaaaccg tatagtatgc cgacacattg
      421 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
      481 attcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
      541 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
      601 ttatgaaagc atattttgac aagatggaat caaatggcat cagtgttatg gcagccatat
      661 tactggtgga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
      721 gtacccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatca
      781 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
      841 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
      901 cacttccgaa agaacagcta ttgatgttaa aaggatctgt ggatgaaata acccctccat
      961 tatcaccagc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
     1021 tgatgattca gctccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
     1081 atagtggaca tatgaacgtt attaatacta tttttaacgc attacccact ctgtttaata
     1141 cgtttaaatt cgataaaaaa atatgaaacc cctcctcctg gcaaataatt ctaatgaata
     1201 tcccggtttg ttttcagcga tacagcataa acaacaaaat gttgtagaga cggtttatct
     1261 tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
     1321 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat tgggtcaccg
     1381 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
     1441 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
     1501 tccccatacc gtacgctaaa tctttgtctg cgccgatacg cataaacatc ttccacatgc
     1561 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
     1621 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
     1681 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
     1741 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
     1801 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
     1861 gggaaaatgt tagcggcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
     1921 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctca
     1981 atataaagat ggcgaatcac aatttgttcg ccaagatcgg tgattttttc cgctgcttcc
     2041 tggagcagta attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca
     2101 aactcctggg tgtccgccat tcggcccacg cgatcgtgct ctttcaccag ttgatagcag
     2161 gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgactttg
     2221 aatacccgat caaagcccgg cagcgtaaac accaacatca ccattccacg aatacccggc
     2281 gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg
     2341 gttttggcgt gcttctggca gccgatagcc atatacaatt cagcagtggt tttacctggc
     2401 agaatttccc gtagccactc gaccagcgct gcgggcagcg gtgcataaac cataaaataa
     2461 gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata
     2521 aataactcgc cgtcgtccgt ctggtggatc ggcagtaaga agggcaacgt gccggaaggc
     2581 gtgatcagtt tgcccaccag ccaggcggct ttattgcggt aaaacagttc gttcgccacc
     2641 tgtaaatgac tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg
     2701 taatgaatgt cacggctttt attctgccag cgcaggcgca gcggcaggtc gctgataacg
     2761 cgcatcagca gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga
     2821 atggtacgaa agcggcgctc tggctgggag ctaaagataa aaagccgctc gggagtaagc
     2881 gagcggtggt caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag
     2941 cgcgggtaat ccggcaacag ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg
     3001 tccgtacttt ggccgttagt aatgcagcgc agttgctcca cgaccagacc aacgtgatga
     3061 tcgtaaagat ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcctgttcg
     3121 aaacgctgct gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct
     3181 tgcaaaatgg tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag
     3241 gagcagagaa ttgccggatg cggcgtaaat gccttatccg gcctacgttc ggcaacggtt
     3301 gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg
     3361 gcgtgaacgc cttatccggc ctacagtcag caacggttgt tgttgcttag aactgcgatt
     3421 cttcggtgga gccggtcagc gctgtaaccg acgacgtgcc gccctgaata atagtcgtca
     3481 ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt
     3541 tcgcggcggc aaattccggc tgctgcactt tctcaacgta gtgcttcata ccctcgccct
     3601 gggcataggc gtttgccagg tcaaacatgt tgaaccacat gctgtggata cctgccaggg
     3661 tgatgaactg aaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt
     3721 tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc
     3781 ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg
     3841 aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt
     3901 gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgcca gtaataaatt
     3961 cactgtcata cgggtcgcaa tcggaggtaa tcagatccgc cgcatcggca tcggtacgag
     4021 caaccagcag ggttggaacg cccgtcacgt cagctgcaag acgcgccgcg accagtttct
     4081 gaatagcttc ctgagttggc accaaaactt tgccgcccat gtgaccgcat ttcttcactg
     4141 acgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc gctttcatca
     4201 gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga
     4261 agtaatcgac atagcgcgga tcgcccggct caatgcctgc ggaccattgg atctgatcgg
     4321 cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga
     4381 gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg
     4441 acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg
     4501 caccgaggct gttgatgtag ccttttttcg actcgccatg cagcagacgc cacattttcg
     4561 ccgcgcccag ttgcgccagg gtgcattcag gattgactga accgcgtaat ttcaccacat
     4621 cttccgcgct gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta
     4681 attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt
     4741 ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt
     4801 gatctgttcc atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc
     4861 cagttcgctg gcaatgactt tcatctcttc acccagcatc tggcggaaca aggctttggt
     4921 caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga ttgaggtacg
     4981 ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc
     5041 ggagatccat gcttcgatgt actgcactgc cacgcgaatg ttggcgcgca taccttcttc
     5101 ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc
     5161 gcgcatcact tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc
     5221 cgtatccgca aggcctgggt gagcaatcca tgtgccatcg tgaccgttat tggcttccag
     5281 cgctttatcc gcttttactt tgttgagcac ctggttattg cgctcttcat ctttgctcgg
     5341 aataaacgct gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa
     5401 caggcgtgag taagcattca ggaatggttt atccatcgtc actgcctgtc tgtctggcag
     5461 gacgcgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccaacgacc
     5521 gcagttcaga ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca tctggaacac
     5581 ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg
     5641 atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgccagg actgggtttt
     5701 cggcagatag aaatagggac cactgccctt tgccaacagt gcctgatagt tgtggaagaa
     5761 atagagcgca aaatcaaaca ggctgccggg gattgcctca ccacgccagg tgacatgttt
     5821 ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg
     5881 gtaaattttg cctgcttcat tggtgtaact gatggtgccg ttaaccgcat cacgcaggtt
     5941 aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat
     6001 aaagactttc acattggcgt tgagcgcgtt gatcaccatc ttgcgctcta ccgggccagt
     6061 tatctctacg cggcggtctt ctaagtccgc aggaatcccg cgaattttcc aatcagcatc
     6121 gcgaatggaa gctgtttccg aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg
     6181 ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc
     6241 agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct
     6301 tgtgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat
     6361 ggatccgaaa acttccccac tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa
     6421 ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat taaaaatact
     6481 tgataacttg ttgataacaa tatgaataaa atttattcac ctgctgatgt acgtttcgga
     6541 aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta
     6601 tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc gtgatctggt
     6661 agacgtaata gttgagccag ttggtaaaca gtaaattacc gtgactacgc cagctcgctc
     6721 gcggtgtatt ttgcggatca ttgtgcggga aatagttata cggtacatcc gggtctagtc
     6781 cggcttccac atcgcggaaa aattcctgcg ccagcgtttg cgcatcatat tcgggatggc
     6841 ccgtcacaaa ggcaatgcgc ttatctttac tggcaaacag atatgcatcc ccttcttccg
     6901 tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat
     6961 agcgcgaatg cggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat
     7021 ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc
     7081 cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt
     7141 gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca tcattaaact
     7201 ccaccaggcc cagcggcgca ccagttacaa tcaaaccgtc aaagttctga tcctgaatat
     7261 cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc gattcacggg
     7321 aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa
     7381 actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc ttaagtggac
     7441 gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct tcacgcaaga
     7501 aattgacggc gggtagctcg tccggcacac gaatcggcat aacctgatta cctcactaca
     7561 tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg
     7621 agcctgcatg ttgaaggtga gaaagtttca aggaaaagac tacgttagaa tataagaatg
     7681 actaaaagga gagaaaatgg ttattagtat tcgccgctca cggcatgagg aaggggagga
     7741 actcgttgcg atttggtgtc gttctgtcga tgccactcac gattttctat cagcagagta
     7801 tcggaccgag ctggaggacc tagttcgttc cttcctgccg gaagcgccgt tgtgggtcgc
     7861 ggttaatgag cgggatcagc cggttggatt tatgttgcta agtgggcagc atatggatgc
     7921 gctgtttatc gatcctgatg tgcgcggctg cggcgtaggt cgggtgctgg tggagcatgc
     7981 gctctcgatg gcaccggaac tgacaaccaa cgtgaatgag caaaatgagc aggcggttgg
     8041 gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa
     8101 accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgccccct atcgtgctat
     8161 cgtgcctgcg taagctggat aagacgcgcc agcgtcgcat ccggcatttt tttctgcgcc
     8221 aatgcaaaaa ggccatcctg acggatggcc tttcgcttta tttgatgcct ggcagtttat
     8281 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc
     8341 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa a
//
LOCUS       DACAPB010000187         8387 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.187, whole genome shotgun sequence.
ACCESSION   DACAPB010000187 DACAPB010000000
VERSION     DACAPB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.187"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            571..846
                     /locus_tag="IT904_003912"
     CDS             571..846
                     /locus_tag="IT904_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193621.1"
                     /translation="MKPKETCEQKTRTRKRLRRWRFSIGSAPLTSIIKIDAQVRGGET
                     RQDYKDTRRFPLEAPSCALLFLPFGLPVSFRCYGRVYLIPRLTLSSG"
     gene            871..1092
                     /locus_tag="IT904_003913"
     CDS             871..1092
                     /locus_tag="IT904_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP3193622.1"
                     /translation="MHEPPVQSDRCAFSGNYQLESNPERHDKAPLAAATGKQDVREMY
                     REFLKWWPDCGYTERTDLVTCLAQDCYHG"
     gene            1315..1746
                     /locus_tag="IT904_003914"
     CDS             1315..1746
                     /locus_tag="IT904_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193623.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTP
                     GYTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSR
                     SGGNPELSRHIDLMKNELKENHEHLDESLKRLAAELKRLGY"
     gene            1830..2159
                     /locus_tag="IT904_003915"
     CDS             1830..2159
                     /locus_tag="IT904_003915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193624.1"
                     /translation="MKILPAEPGLYFFASFESGAHVYKVVAWSENENGGLDPQCVLTA
                     LYDKSSSSQQWELHSTSASAYQHSNDGRPFMYFITYFTSNGGIPELYNAINTLAERWG
                     YPLIDLP"
     gene            complement(2677..5643)
                     /locus_tag="IT904_003916"
     CDS             complement(2677..5643)
                     /locus_tag="IT904_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HAP3193625.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP
                     LIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSR
                     RLEDGKFRPLRMPGKP"
     gene            complement(5647..6207)
                     /locus_tag="IT904_003917"
     CDS             complement(5647..6207)
                     /locus_tag="IT904_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3193626.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            6196..6363
                     /locus_tag="IT904_003918"
     CDS             6196..6363
                     /locus_tag="IT904_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193627.1"
                     /translation="MPLHDVSLVEGGLSALSVPLCLKAHFCQAEIYNLRGHVSKNGKA
                     DSILTKRRGPA"
     gene            6517..8094
                     /locus_tag="IT904_003919"
     CDS             6517..8094
                     /locus_tag="IT904_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP3193628.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
BASE COUNT         1937 a         2274 c         2343 g         1833 t
ORIGIN      
        1 aaaaaaaatg aatgtttcat tttcacgagt aatacccagg tattaaaaac acactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggcgtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cggcccagaa tccacagggg catgcaggat
      181 ctcttcagta accttcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaaccc cgttgttcat catattcaga tggcatgggg tgatatccag tagcagagag
      301 attgttagcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
      361 actggcttaa ctatgcagca tcagtgcgaa ttgtatggaa agcgcaccat gtccggtgtg
      421 aaatgccgca cagatgcgta aggagaaaat gcacgttccg gcgctcttcc gcttcctcgc
      481 tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggtgtctgct cactcaaaag
      541 cggagatgcg gttatccaca gaatcagggg ataaagccaa aagaaacatg tgagcaaaaa
      601 acgagaaccc ggaaaaggct gcgccgttgg cgtttttcca taggctccgc ccccctgacg
      661 agcatcataa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttcctgcc attcggttta
      781 ccggtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcagttcc
      841 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
      901 tgcgcctttt ccggtaacta tcaacttgag tccaacccgg aaagacacga caaagcgcca
      961 ctggcagcag ccactggtaa acaggatgtg cgagagatgt atcgagagtt cttgaagtgg
     1021 tggcctgact gcggctacac tgaaaggaca gatttggtga catgtctcgc acaagactgt
     1081 taccacggtt agagaatctg ccaggattaa ctaaccttcg aaaaaccacc tgccagggtg
     1141 gttttttcgt tttctgagca agagattacg ctctgccata aatgatctca agaagatcca
     1201 cgccaacaac ttcaaatcca tcaattcaca aaatgttaca cttactaatt agtaaaataa
     1261 gctgttttgt tattttgtta attaacaacc taaattcaca taaaagagaa tgatatgaac
     1321 atccctactc atctgaaaca caaaccgatt atcgctgtag aaaactatga tcgaattgat
     1381 ggaccttatg ctgatgacac tgatgctatg gggctttccg ttggaattgc tcaatggaac
     1441 acccccgggt ataccgagct atcagcaaaa gtgtggcgca acaccggaga aaagtggtca
     1501 cggcaatcag aagaattacc tcttcatcgt gttatcgact tggcaacgct tatctgcata
     1561 gcaatggatt acagtaaaca tggccgctta tcttccagcg acgactttcc ggtcagtcgt
     1621 tcaggaggca atcctgagtt atctcgtcac attgatttaa tgaaaaacga gctaaaagag
     1681 aatcacgagc atttagatga gagtcttaag cgtttagcag cagaactaaa acgtttaggt
     1741 tattaacctc ccagtgattt tttataccca ccacggggaa ttaggtagtt attgcctctc
     1801 agacagaata aacaaaagga agctagctaa tgaagatcct accagctgaa ccgggattat
     1861 attttttcgc atcttttgag tccggtgctc acgtttacaa agtagtggcc tggtcagaaa
     1921 atgagaatgg tggtttagat ccacagtgtg tgttaacggc cttatatgac aagagcagtt
     1981 ccagccagca atgggaactt cactcaactt cagcatctgc atatcagcac agcaatgacg
     2041 gcagaccatt tatgtatttt atcacctact tcaccagcaa tggcgggata cctgaacttt
     2101 ataacgcaat aaacacacta gccgaacgat ggggataccc attaattgat ttaccataac
     2161 attcgttgcg tgcaccgccg cagaggccct cagcgcgttt ctgaggcctc cggcctgaaa
     2221 cacacacctt tctgtgccca tatcgagaaa aaacgcccct gatgattctg gcgcgactgc
     2281 atggctatgc acggacatac ctggccggga ttttagcggc taaaatccga tgaacgccct
     2341 gaaagccacc acgaaccacc ccgataacgg tgaacggatg cgcaatttat tgcgtggccg
     2401 tgaggcgttg ccggcacagc cggaaaaacc attttcagca cgtgcaggag gcccaggaag
     2461 aggggcggat gtacgaatag ttcagaggtc agatctgacg taaaaatgat gtaaaagtga
     2521 cgtaaaagtg atgtaatttt gatgtacttt tgatgtaatt ctgtagtcag gaagaataac
     2581 ccgttcatgc aggcatgaac gatacagtga ggaacgcttt ttttgtcacg atctcccctt
     2641 aacggggagc ccgcagaatt cggaaaaaat cgtacgctaa ggttttccgg gcatccgtaa
     2701 gggccgaaac ttcccgtctt ccagtctgcg gctctgccgc cagacgtaat cgccggttag
     2761 gttgatgtgc tcccagccca gcggcgacag gaattgcagc agctcgccgt ccaccggctt
     2821 gccggcctcg accaacccct gggtggcgcg ttccaggtac accgtgttcc acagcacgat
     2881 agccgccgtc accaggttga ggccgctggc ccggtagcgc tgctgctcga agctccgatc
     2941 cctgatttcc ccaaggcggt tgaagaacac cgccctggcc agcgagttgc gcgcctcacc
     3001 tttgttcagg ccggcatgca cgcggcggcg cagttcaaca ctttgcagcc agtccaggat
     3061 gaacagcgtg cgctcgatcc ggcccagctc gcgcagggcc acggccagtc cgttctggcg
     3121 cgggtagctg ccgagcttgc gcagcatcag cgaggcggtg acggtgccct gcttgatcga
     3181 gctggccagg cgcaggatgt cgtcccagtg ggcacgcacg tgcttgatgt tcagggtgcc
     3241 gccgatcagc gggcgcaacg tcgggtaggc ttgcacgccc tgcggcacgt acagcttggt
     3301 ttcgccgagg tcgcggatgc gcggcgcgaa gcggaagcct agcaggtgca tcagggcaaa
     3361 gacgtgatcg gtgaagccgg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggactc
     3421 gtggtacagc aggccgtcga gcacataggt ggaatcgcgg acgccgacat tcaccacgcg
     3481 ggtgctgaac ggcgcgtact ggtcggagat atgggtatag aacagccgtc ccggctcgct
     3541 accgtacttc gggttgacgt gcccggtgct ctcgccccgg ccacccgcgc ggaagcgctg
     3601 gccatcggag gatgaggtcg tgccgtcgcc ccagtgggcg gcaaaggcgt ggcgatactg
     3661 gtggttgacc agctcggcca aggccgccga ataggtttcg tcgcggatgt gccaggcttg
     3721 cagccaggac agcttggcgt aggtcaggcc ggggctcgac tcggccatct tggtcagccc
     3781 gaggttgatc gcatcaccga ggattgcgga cagcagcaac gtcctgtctt tggcctcggc
     3841 cccgtccttc aagtgggtga agtggcggct gaagcccgtc cagtcgtcca cgtccatcag
     3901 cagttcggtg atcttgatgc gcggcagtaa ctggctggtt tggtcgatca gcgcctgcgc
     3961 ccgatccggc accgccgcat ccagcggggt gattttcagc cctgactcgg tgaggatggc
     4021 atcgggcagc tcgttgtcct tggccaggcg ggtgacggtg gccaactgct cgtccagcag
     4081 ctgcaaacgc tcttccaggt actggtcgct gttcgggttg atcgccaggg gcagggcctg
     4141 ctcgcgcttg agtgcggcga acttctcggc cggcagcagg tagtcgtcga agtcgcggaa
     4201 ctgccgcgag cccttgaccc agatgtcgcc ggagcgcagg gcgttcttca gctcggacag
     4261 ggcgcagatt tcgtagaatt tccggtcgag gccttccggg gtgatcacca gcggcttcca
     4321 gcgcggcttg atgaaggccg tgggtgcatc ggccggcacc ttgcgcaggt tgtcggcgtt
     4381 catctcacgc agggtctgca cggctgccag cacgccttgc gcggccggcg cggcgcgcag
     4441 ttccagcacc tccagcaagg ccggcgtgta acggcgcagg gtggcgaagt tctcgccgac
     4501 caggtgcagg tggtcgaagc cttccggccg ggccagcagc tcggcctcgc tgacgctctc
     4561 ggtgaactcg tcccagggaa tcaccgcctc gatggcggca taggggtcgc tgccgctttc
     4621 cttcgcttcc agcagcgcct ggccgatcct ggagtacagg cgcaccttgt cgttgatcgc
     4681 cttgccctgc ttctggaact gctgctgatg cttgtgcttc gcgccgctga acagcttgac
     4741 caggatgcgg tcatgcagat cgaccagctc atcgatcacg gtcgcggtgc tctccagcac
     4801 cacggcggcc agggtcgcgt agcggcgctg cggctcgaac ttaccgaggt ctttgggcgt
     4861 catctgccca ccctcgcggg ccagcttgag caggcggttc tggtggatgt gccggcccag
     4921 gccttcgggc aagtccacca actgaaatgt cttcagccgc tcgatgtgtt ccagcatgtg
     4981 ccgagagttg ggtttcagcg gtgcctggcg cagccaggtc aaccaggtga tgctgctgcc
     5041 ggccttgagc ttcaacagct cgtccagctt ggcccgatgc gagtccgtga gtggttcgac
     5101 cagggcgcgg tagacccgcc gattggctcg cgcaatggct tccgagcagg cccggtcaat
     5161 cacgctcagc gccggcagga tgcgtcgttt ctgccgtagg ctctccaggg cctgaccggc
     5221 cagcagcaag cctttgtcgg tctgctgggc cagctcggtt agctcgcgca ccagggcgcg
     5281 gaagtcggac aggccgaacg gggccagttg caggtaggtg cgcagttcct gggcatgctc
     5341 gcgacgggtc acgtcgcgct cgccgtactt tgcccagctc gccggctcgg cctggacttg
     5401 cttcgccacc cacaggatga ccggctcggg cagctcgctg tcggttccca gcgcgtaacc
     5461 ggggtagcgc agcaggcaga gctgcacggc gaagccgagg cggttggcgt cgccgcgtcg
     5521 ctggcggatc agcgacaggt cggagtcgtt gaaggtgtag tagcggatca ggtcatcctg
     5581 gctttccggc agcgcaagca gggtgtcccg ctccgtggcc gagaggatca agcgacgcgg
     5641 catgtgtcag tcgtccgtgc ggaggtactg gtagagggtt tcccggctga tgttgaactc
     5701 gcgggcaagc tgcgccttgg gctcgccggc cgtcgctcgc tgccgcaggg tagcagcctg
     5761 ctcatcggac agggctttct tgcggccccg gtacgcgcca cgctgcttgg ccaaggcgat
     5821 gccctcacgc tgccgctcgc ggatcagggc gcgctcgaac tcagcgaagg cccccatcac
     5881 cgacagcatc aggttggcca tcggcgagtc ctcgccagtg aacaccaggc cctccttcag
     5941 gaactcgatg cgcacgccgc gctgagtcag cttctgtacc aagcgacgca ggtcatcgag
     6001 gttgcgggcc agccgatcca tgctgtgcac caccactgta tcgccttcgc ggacgaagct
     6061 cagcagcgct tcgagctggg ggcgctgggt gtccttgccc gatgccttgt cggtgaacac
     6121 cttgctcacc tgggtttgtt ccagctggcg ttccgggttc tggtcaaagc tgctgacccg
     6181 gacgtagccg atgcggtgcc cctgcacgat gtctccttgg ttgaaggcgg cttaagtgca
     6241 ctttctgttc cgttgtgcct caaagcccat ttctgtcagg ctgaaatcta taaccttcgc
     6301 gggcatgtgt caaaaaatgg gaaagcagac tctattctga cgaagcggcg cggccctgcc
     6361 tgacatcaag ttagggtata gcctagattg acatgcgcga tgcaaccctt aacttgcttg
     6421 cacctatcgt ttccatgcta gctttatcgt aacgctcaag aaattgggct taatgccgaa
     6481 aacaataaaa taaaacagcc aacccttgag gttcttatgc gccagaattt accagtgacg
     6541 ggtcgaaact tagaactccc aaaagatgcc aatattcttt cgactacctc ccctcaaagc
     6601 catatcacgt acgttaatcc tgacttcatt aaaatcagtg gtttcactga ggaagaacta
     6661 ttaggccagc ctcacaacat cgtaagacac ccagatatgc cgcctgctgc atttgagcat
     6721 atgtggagta cattaaaatc tggccgctca tggatggggc tagtaaaaaa tcgctgtaaa
     6781 aatggcgacc actattgggt aagtgcttat gtaacgccaa tagctaagaa tggttcgatt
     6841 gttgaatacc agtctgtaag gaccaagcct gaacctgagc aggttttggc tgcggaaaaa
     6901 ttatatgctc aattgagaag cgggaaggcc gcgaggccga aattggctgc tagcttttcc
     6961 gtgaaaatac tcttgctcat atggggtagt attatatcaa gcgcaatggc tgccggcatg
     7021 cttactgata catcaataag cagcttattg ttagccactt taatgtcagg aagcttaagc
     7081 tctgttagtg ttttggctat tctctctcct cttggaagac tggttgaaag agccaggaat
     7141 atttccaata acccattaag tcaatccctc tacactgggc gcaccgatga gtttggccaa
     7201 atagagtttg ctttacgaat gatgcaagct gaaacaggcg ccatagtagg tcgcataggt
     7261 gatgcatcaa atcggcttag cgaacacacc cgaggcctac taaaggatat tgagtcaagc
     7321 aatgtactta cagttgagca gcaggcagag acagatcaaa tagcaacggc agtaaaccaa
     7381 atggtggcaa gcattcaaga ggttgcgagc aatgcacagc atgctgcaga tgcggccgga
     7441 agagcagaca ctgagacggc atctggccag cgtctggtag cccacacaag ccagtcaatc
     7501 actgcccttg aaggtgaaat taggcaagcc actcaggtta ttcatgagct tgaaggtcaa
     7561 agtaacgaga tatcaaaagt tcttgacgtt atacgaggga tcgccgagca aacgaatttg
     7621 ttggcactca acgcagcaat tgaggccgcg cgtgctggtg agcaggggcg tggttttgct
     7681 gttgtcgccg atgaggttcg cagtcttgct gctcgcacac agcaatcgac aacggatatt
     7741 caaagcatga tcagcgctct acaagagcga gcgcaatccg ctgttacagt catggagcaa
     7801 agtagtcggc aagcgcacac gagtgtagct cacgcagagg aagcagctac agctcttgat
     7861 ggaattggcc aacgcgttaa cgaaattacc gacatgaacg cgcaaatagc gactgcggtc
     7921 gagcagcagg gagcagtaag tgaagacata aaccgcagta ttatcaatat acgcgatgct
     7981 gctgatacca atgtacagac cgggcagaat aatttgcaaa gtgcgaaatc tgtcgctcag
     8041 ttaactagcg ctctgagcga actggcaaaa cagttttggg aaaaacgagg ataacgcttt
     8101 tcagtatctc gacagggata acttacttta ccatcggtta tcccttttag ccgcacaaat
     8161 tttagccatg gcttttcagg aagtcagggc tatcagaatg gccttagaaa gcctagtcaa
     8221 agagctgtca cgagaacacc gttagcttag cgtacgattt tttccgaatt ctgcggttcc
     8281 cccttaacct gcgtaaatca ccctccatag ttgattattt ttcaacctca aaatagtcat
     8341 acacctaacg cagccgcgat tttcatcgca actgcatgaa ttaaaag
//
LOCUS       DACAPB010000188         8292 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.188, whole genome shotgun sequence.
ACCESSION   DACAPB010000188 DACAPB010000000
VERSION     DACAPB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.188"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..2753
                     /locus_tag="IT904_003920"
     CDS             <1..2753
                     /locus_tag="IT904_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP3193629.1"
                     /translation="TVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFH
                     LWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIF
                     RDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQV
                     ASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEA
                     EACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNN
                     FNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVT
                     RMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDII
                     CPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFR
                     QYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHP
                     ALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVA
                     PQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLP
                     SASHIIPQLTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPL
                     DNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGK
                     TLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQEN
                     YPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYS
                     QQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWC
                     QK"
     gene            2805..4058
                     /gene="lacY"
                     /locus_tag="IT904_003921"
     CDS             2805..4058
                     /gene="lacY"
                     /locus_tag="IT904_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HAP3193630.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            4124..4735
                     /gene="lacA"
                     /locus_tag="IT904_003922"
     CDS             4124..4735
                     /gene="lacA"
                     /locus_tag="IT904_003922"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HAP3193631.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITTGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(4838..6019)
                     /gene="cynX"
                     /locus_tag="IT904_003923"
     CDS             complement(4838..6019)
                     /gene="cynX"
                     /locus_tag="IT904_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HAP3193632.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGVGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSMQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(6025..6495)
                     /gene="cynS"
                     /locus_tag="IT904_003924"
     CDS             complement(6025..6495)
                     /gene="cynS"
                     /locus_tag="IT904_003924"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HAP3193633.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(6526..7185)
                     /locus_tag="IT904_003925"
     CDS             complement(6526..7185)
                     /locus_tag="IT904_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAP3193634.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            7295..8194
                     /gene="cynR"
                     /locus_tag="IT904_003926"
     CDS             7295..8194
                     /gene="cynR"
                     /locus_tag="IT904_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HAP3193635.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMANFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
BASE COUNT         1941 a         2176 c         2234 g         1941 t
ORIGIN      
        1 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
       61 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
      121 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
      181 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
      241 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
      301 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
      361 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
      421 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
      481 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
      541 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagtgcc gaaatcccga
      601 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
      661 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
      721 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
      781 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
      841 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
      901 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
      961 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
     1021 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
     1081 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
     1141 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
     1201 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
     1261 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
     1321 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
     1381 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
     1441 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
     1501 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
     1561 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
     1621 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
     1681 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
     1741 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
     1801 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
     1861 gtgtgacgct cccctccgcg tcccatatca tcccgcaact aaccaccagc gaaacggatt
     1921 tttgcatcga gctgggtaat aagcgttggc agtttaaccg tcagtcaggc cttctttcac
     1981 agatgtggat tggcgatgaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgcg
     2041 caccgctgga taacgacatt ggcgtaagtg aagcgacccg tattgaccct aacgcctggg
     2101 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc ggcgttgttg cagtgcacgg
     2161 cagatacact tgccgacgcg gtgctgatta cgaccgctca cgcgtggcag catcagggaa
     2221 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
     2281 ccgttgatgt tgaagtagcg agcgatacac cgcatccggc tcggattggc ctgacctgcc
     2341 agctggcgca ggtcgcagaa cgggtaaact ggctcggatt agggccgcaa gaaaactatc
     2401 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
     2461 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
     2521 gcccacacca gtggcgcggc gacttccagt tcaatatcag tcgctacagc caacaacaac
     2581 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
     2641 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
     2701 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
     2761 cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa
     2821 acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct
     2881 acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg
     2941 gtattatttt tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc
     3001 tttctgacaa actcgggctg cgcaaatacc tgctgtggat tattaccggc atgttagtga
     3061 tgtttgcgcc gttctttatt tttatcttcg ggccactgtt acaatacaac attttagtag
     3121 gatcgattgt tggtggtatt tatctaggct tttgttttaa cgccggtgcg ccagcagtag
     3181 aggcatttat tgagaaagtc agccgtcgca gtaatttcga atttggtcgc gcgcggatgt
     3241 ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc accatcaata
     3301 atcagtttgt tttctggctg ggctctggct gtgcactcat cctcgccgtt ttactctttt
     3361 tcgccaaaac ggatgcgccc tcttctgcca cggttgccaa tgcggtaggt gccaaccatt
     3421 cggcatttag ccttaagctg gcactggaac tgttcagaca gccaaaactg tggtttttgt
     3481 cactgtatgt tattggcgtt tcctgcacct acgatgtttt tgaccaacag tttgctaatt
     3541 tctttacttc gttctttgct accggtgaac agggtacgcg ggtatttggc tacgtaacga
     3601 caatgggcga attacttaac gcctcgatta tgttctttgc gccactgatc attaatcgca
     3661 tcggtgggaa aaacgccctg ctgctggctg gcactattat gtctgtacgt attattggct
     3721 catcgttcgc cacctcagcg ctggaagtgg ttattctgaa aacgctgcat atgtttgaag
     3781 taccgttcct gctggtgggc tgctttaaat atattaccag ccagtttgaa gtgcgttttt
     3841 cagcgacgat ttatctggtc tgtttctgct tctttaagca actggcgatg atttttatgt
     3901 ctgtactggc gggcaatatg tatgaaagca tcggtttcca gggcgcttat ctggtgctgg
     3961 gtctggtggc gctgggcttc accttaattt ccgtgttcac gcttagcggc cccggcccgc
     4021 tttccctgct gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg
     4081 cgagcgcctt atccgaccaa catatcataa cggagtgatc gcattgaaca tgccaatgac
     4141 cgaaagaata agagcaggca agctatttac cgatatgtgc gaaggcttac cggaaaaaag
     4201 acttcgtggg aaaacgttaa tgtatgagtt taatcactcg catccatcag aagttgaaaa
     4261 aagagaaagc ctgattaaag aaatgtttgc cacggtaggg gaaaacgcct gggtagaacc
     4321 gcctgtctat ttctcttacg gttccaacat ccatataggc cgcaattttt atgcaaattt
     4381 caatttaacc attgtcgatg actacacggt aacaatcggt gataacgtac tgattgcacc
     4441 caacgttact ctttccgtta cgggacaccc tgtacaccat gaattgagaa aaaacggcga
     4501 gatgtactct tttccgataa cgactggcaa taacgtctgg atcggaagtc atgtggttat
     4561 taatccaggc gtcaccatcg gggataattc tgttattggc gcgggtagta tcgtcacaaa
     4621 agacattcca ccaaacgtcg tggcggctgg cgttccttgt cgggttattc gcgaaataaa
     4681 cgaccgggat aagcactatt atttcaaaga ttataaagtt gaatcgtcag tttaaattat
     4741 aaaaattgcc tgatacgctg cgcttatcag gcctacaagt tcagcgatct acattagccg
     4801 catccggcat gaacaaagcg caggaacaag cgtcgcatca tgcctctttg acccacagct
     4861 gcggaaaacg tactggtgca aaacgcaggg ttatgatcat cagcccaacg acgcacagcg
     4921 catgaaatgc ccagtccatc aggtaattgc cgctgatact acgcagcacg ccagaaaacc
     4981 acggggcaag cccggcgatg ataaaaccga ttccctgcat aaacgccacc agcttgccag
     5041 caatagccgg ttgcatagag tgatcgagcg ccagcagcaa acagagcgga aacgcgccgc
     5101 ccagacctaa cccacacacc atcgcccaca ataccggcaa ttgcagcggc aaccagataa
     5161 agccgcagaa ccccaccagt tgtaacacca gcgccagcat taacagtttg cgccgatcct
     5221 gatggcgagc catagcaggc atcagcaaag ctcctgcggc ttgcccaagc gtcatcaatg
     5281 ccagtaagga accgctgtac tgcgcgctgg caccaatctc aatatagaaa gcgggtaacc
     5341 aggcaatcag gctggcgtaa ccgccgttaa tcagaccgaa gtaaacaccc agcgtccacg
     5401 cgcggggagt gaataccacg cgaaccggag tggttgttgt cttgtgggaa gaggcgacct
     5461 cgcgggcgct ttgccaccac caggcaaaga gcgcaacaac ggcaggcagc gcccaccagg
     5521 cgagtgtttg ataccaggtt tcgctatgtt gaactaacca gggcgttatg gcggcaccaa
     5581 gtccaccgcc gcccatcaga gccgcagacc acagccccat caccagtggc gtgcgttgct
     5641 gaaaccgccg tttaatcact gaaggcatca ccgcctgaat gataccgacc cccaccccac
     5701 caagcagtgc gctgctaagc agcagcacac tttgggggta aagctcacgc atcaatgcac
     5761 cgacggcaat cagcaacaga ctgatggcga cgctgcgacg ttcgctgaca tgctgatgaa
     5821 gccagcttcc ggccagcgcc agcccgccca tggtgaccac cggcagagcg gtcaacaggg
     5881 cagccacgct aaagctcatt ccgctcgcct ggcgcaattg cggtagcagt ggaccgacgg
     5941 aggtgagcag tggtcgcata ttaagaccaa tcagcaccag aaccagcagc atcgtgaacg
     6001 tcttttgatg gttgcgcatg gctgtcagaa cggtttggtc ggcagatatt taccatctaa
     6061 ggtgatgacc gcacgttcgc caccttccgg gtccgccact ttcttaacgt cgagtttgaa
     6121 gttaatcgcg ctgataatgc catcgccaaa cttctcatga accaacgctt tcagggttgt
     6181 gccgtacacc tgcaacattt catagaaacg atacatcgtt gggtcggttg gaatacggtc
     6241 atcaatgcag ccacgcagtg gaatcatctg caacagtaga atggcgtctt cgtcgagatc
     6301 cagcttcgcc ccgaccaggc gggcggcgtc ggcaggaagc gcctgctgcc ccagcaaagc
     6361 cgcggttacg aaggcttctg ccagaccggt accgtcggca atctcggcaa atgagagatc
     6421 ttttttagct ttgctgagca aaatggcatc ggcaagatca agacgaatat tgcggttaat
     6481 ttgtgactga atcatggtgg atctcctgat ggtttaaaaa taaggttacg ctgcggtagg
     6541 ttggcgtagc ggtatggcac aaacgcgagg attagcggcc agtggcacaa actggcgggt
     6601 tgcgccgtca aaagctgcga tgctgccgct ttcaatgtcg tagacccagc cgtgcagggc
     6661 gatccgcccc tcttcgagcg ccaggcgcac cgatggatga gtttgcaaat tagccaactg
     6721 agcaatgacg ttttcacgta ccatcgccgc agcttttgac ggtaaatcgg aatgcgggcg
     6781 cgcctcatta acgacgcggg ctgaatcggc ataacgcagc cagtgggaga cggcaggcat
     6841 atggtccata cactgacagc tggcaatggc ggtcatcgcg ccacagttgg aatgaccaca
     6901 aatcacaatg tcagataccc gaagcgcagc gacggcatac tccaccgaag cagaaacgcc
     6961 accgggttcc ggcccgtagg aagggacaat attacctgcg ttgcgaataa cgaacagatc
     7021 gccaggctca cgttgcgtca ccagctcagg gaccagacgg ctgtcggagc aggagataaa
     7081 aagggtgcgc gggctttgct gtgtcgccag ctgtttaaat aaggcttccc gcttcggaaa
     7141 tgcctcgcgc tggaatttaa ggaatccatc aataatctct ttcaccgtta acctctgtct
     7201 gtctctggaa tgagaggcag actactcgcc gattgtcata aggtaaaagt ctcatttatg
     7261 atgagttcta ttggatttac ttataggttg gcgaatgctc tctcgacata tcaactattt
     7321 tcttgccgtg gctgaacatg gcagctttac ccgtgccgcc agtgcgttgc acgtctccca
     7381 gcctgcgctt tcccagcaga ttcgccagtt agaggagagt ttaggcgtgc cgctgtttga
     7441 ccgtagcggg cgaacgattc gtctcactga tgcaggagaa gtctggcgac agtacgccag
     7501 ccgggcgtta caggaactgg gagcgggtaa acgggcgatt catgatgttg ccgatctgac
     7561 gcgaggatcg ctgcgtatcg ccgtcactcc cacctttaca agctacttta tcggcccttt
     7621 aatggcgaat ttctatgcgc gctaccccgg catcacgctc cagctacagg aaatgtcgca
     7681 ggagaaaatc gaggatatgc tttgccgcga cgagttggac gttgggattg ccttcgcgcc
     7741 tgtgcattcg ccggagctgg aggcaattcc tttactgaca gaaagtttag cgttagtcgt
     7801 ggcgcaacat catccgctgg ccgtccatga acaggtggcg ttgagtcgct tgcatgatga
     7861 aaaactggtc ctgctcagcg cggaatttgc caccagagag caaattgacc actactgcga
     7921 gaaagcgggg ctacatccac aggtggtcat tgaggcgaac tcaattagcg cggttctgga
     7981 gctgattcgc cgcacttccc tttccacatt gttaccagca gcgattgcca cacaacatga
     8041 cgggcttaaa gctatttctc ttgccccgcc actactggag agaacggcgg ttttgttgcg
     8101 gcggaaaaat agctggcaga cagccgccgc gaaggcattt ttgcacatgg cgttggataa
     8161 atgcgcggtt gttggcggaa atgaatcacg gtagccgctg acacaggtct gcggctggaa
     8221 ttgcctgatg cgctacgctt atcaggccta cgagtgcagt gctctacgta ggccggataa
     8281 ggcgttcacg cc
//
LOCUS       DACAPB010000189         8095 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.189, whole genome shotgun sequence.
ACCESSION   DACAPB010000189 DACAPB010000000
VERSION     DACAPB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.189"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            59..118
                     /gene="ibsE"
                     /locus_tag="IT904_003927"
     CDS             59..118
                     /gene="ibsE"
                     /locus_tag="IT904_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAP3193636.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(238..1671)
                     /gene="hldE"
                     /locus_tag="IT904_003928"
     CDS             complement(238..1671)
                     /gene="hldE"
                     /locus_tag="IT904_003928"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAP3193637.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1719..4559)
                     /gene="glnE"
                     /locus_tag="IT904_003929"
     CDS             complement(1719..4559)
                     /gene="glnE"
                     /locus_tag="IT904_003929"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAP3193638.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(4582..5883)
                     /locus_tag="IT904_003930"
     CDS             complement(4582..5883)
                     /locus_tag="IT904_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAP3193639.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6125..6745
                     /locus_tag="IT904_003931"
     CDS             6125..6745
                     /locus_tag="IT904_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAP3193640.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLSNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6809..8047
                     /gene="cca"
                     /locus_tag="IT904_003932"
     CDS             6809..8047
                     /gene="cca"
                     /locus_tag="IT904_003932"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAP3193641.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
BASE COUNT         1839 a         2362 c         2065 g         1829 t
ORIGIN      
        1 aaggggctga aacgggaaag cccatcccga agaaggggcc ttgtataagg aaagggttat
       61 gatgaagttc gtcatcatac tggttgtgtt gttactgtta agtttcccga cttactaaca
      121 actcatcaga ggggggagaa atcctccctt acccttgttc ctttactcta ggttgaaaaa
      181 acaacagcgt caataggcct gccatgtacg aagcgagatc tgtgaaccgc tttccggtta
      241 gcctttttta tcctgttgga tcttcttgat gatgttggtc gtcgagcaac cgtcttcaaa
      301 gttgagcacc aacacttcgc caccgttggc ccagacttct ttactcccgg caatctcttc
      361 tggtttatag tcgccgcctt tcaccagcag atctggcaag atcccggcga tcaagcgctg
      421 cggcgtgtcc tcttcaaacg acaccaccca gtcgaccgct tccagtgcgc ccagcacaat
      481 catacgctgt tcgagcgggt taacggggcg ggaatcccct ttcagccgtt tggtggaggc
      541 atcgctgttc acagcgacaa tcaaacggtc acccagttta cgggcatttg ccagataaga
      601 gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc atcaccactt tttcaccacg
      661 tttacgcgct gccgctacgg ccagcttcag ttcctcttcg gtcatcacgc caaagcctgt
      721 atctgcacgt ccacgtacag cattttccag ctcgatcggc gaaaccgtgg aggttcccag
      781 tttgccgacc accacgccag ccgccgcatt ggcaaagaag caggcttctt ccagcgaatt
      841 acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg tcgcccgcac cggtaacgtc
      901 atacacttcc tgcgcttggg ttggcatatg cagcggcgct ttacccggtt gcagcagcga
      961 cataccctgt tcggaacggg tcactaacag agccgagagt tcgtaatcgg caatcagttt
     1021 catgccgcgc tcaacaatct cttcttcggt cttacattta ccgacaacag cttcaaattc
     1081 cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc tcaaaatcgg taccttttgg
     1141 atcaatcagc accggaacac ccgctttacg cgccagttgg atcatctgct gtacgcttgc
     1201 cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca atcgaactca gcgcctgatt
     1261 aatccgctcg tgcagcggct gcggatcaac accttcgaaa ccttcttcaa aatccagacg
     1321 gatcagctgt tggttgcggg aaagtacccg taatttggta atggtcggat gcgtcggtac
     1381 agaaacgaag tcgcatttga cgttgacgtc ggccagagat ttactcagcg cgcgcgctgc
     1441 atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca ccgagagaag cgatattcat
     1501 cgccacgtta gccgcgccgc ccggacgttc ttcgatggta ttcactttaa ccacgggcac
     1561 cggcgcttcc ggcgagatac gactggtggg gccgtaccag taacgatcca gcatcacatc
     1621 accaaccacc atcactcctg cacgttcaaa ctctggcagc gttactttca ttcctgtctc
     1681 ctgagagatt caaaatttgc gcgcgataat accatacttc attcttccac cagccacttc
     1741 tgccagcttg cccgcaccag ttcacgctct gcggtgaagc aatcctccga cacatggccc
     1801 ggcaattcct gtaatgccag atgatgaagt tcatcgcgaa gcgtagtgta agcacgggtc
     1861 agcgccatcg cttcctgctc ttccataatg tcgttttgcg ccagtagttc cagaatacgc
     1921 acgttgtctg accagcgcgt taacttcggt ttttcatgag cgtagcgcaa caccagatat
     1981 tgggtaataa attcgatatc ggtaattccc ccttcatcag ctttgatatc aaagcgatcg
     2041 cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca tttcccgcac ttccgtttgc
     2101 agagttttac cttcacgcgg cagcgtcata atctcgcgac gcactgcgtc aaagtgcgcg
     2161 gtgagctgcg gatcgccgta cactacacgc gcacgcacca gcgcctgatg ttcccacgtc
     2221 caggcctcgt ttttctgata atcggcaaat gcttctgcgg atgtcaccag cattcccgcc
     2281 gccccggacg gacgcagtcg agcatccact tcatacaaaa tgccggaaga ggtacgcgta
     2341 ctgaacagat gcataatgcg ttgcgccaga cgcagataaa actgccgccc gtcgatttcc
     2401 cgctcaccgt cagtcatcgc atccattggg caatcatgga ggaagataag gtcaagatcg
     2461 gaactgtagc ctaactccca gccgcccagc ttgccgtagc cgaccaccgc aaaaccacgc
     2521 ccttcgcgtt cgttcaggtg attcggctta ccgtagcggg caaccatttg aacccacgcc
     2581 tgctgaacga cggcatctat catggcttcc gccagccagg ttaagtgatc gctcactttc
     2641 atcaccggta gcgtaccggc gatatccgct gcggcgatgc gtaacagctg cgcctgtttg
     2701 aactgacgca gcgcctcaag ctgttgctct tcgtcatctt ccggcacgcg cagcaaatac
     2761 tggcgcaact catcgcggta ggcatcggtc gccgtcggct ggtaaagggt gtttggatcg
     2821 agcaattcat ccagcaataa tggataacgc gccagctggc tggcaatcat cggcgacgcg
     2881 gcacacagag aaatcaaatg tttaagcgcc gcggggaatt cactgagcaa ttctaaatag
     2941 gtggtgcggg taacaatccc caccagcaag gcggtaatgc gcgacagcgt aacggcagcg
     3001 tcttcacgcg cacagacatc acttagcaga tgcggcatca gatggtcgag cacctgacgt
     3061 cctcgcggcc cgatggtgcg cttatccagc tctttgcgga aatcggcaat cagcgttagc
     3121 acctgtttgc gatcatcctc gctaagatgc gccagcactg gcgtagtgtc atcttcctgc
     3181 aacgcatcct gccacagctc acgccactgt tccgacagcg actcttcctg agtttcactt
     3241 tcatcgtcgc caatcaattc attaaacacc cggcgcacat tggtcatatg tgcggtcagc
     3301 gccccggtca gttgcggcca gtcagcaaag tccatcgccc acgccagccg cgcacgatta
     3361 agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa tgctttgcag caggttttcc
     3421 agacgccgca ggaacagata cgccactcgc aattgttcag catcgttttc agaaagcaga
     3481 tgcagctcgg caatggcgct gagcgttggc agtaaagagc gcgattgcag cgacggttcg
     3541 cgtccgccgc gaatgagctg gaacacctga acgataaatt caatttcgcg aatgccaccc
     3601 gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca cttcacgggc aatcattcct
     3661 ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt aacgacggaa aacaaacggg
     3721 cgcagcattg cacgcaactc gttagcatag acaccatcgc tatcgcccat aatccgcgcc
     3781 ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct ggtaataatc ctccaacgcg
     3841 gcaaagctca gcaccagcgg gccactttcg ccaaacggac gcagtcgcat atccacgcga
     3901 tagacgaagc catcctgcgt tggttgatcc agcactttaa tcagccgctg ccccatgcgg
     3961 gtaaaaaact gcgcgttatc cagttcccgg cgtccaccct gcgtacaacc atgttccggc
     4021 caggcaaaaa tcagatcgat atctgaggag aaattcagtt ccccaccgcc cagcttaccc
     4081 atgcctaaaa tcagcagcgg ttgcgcttcg ccctgcgcat tgcacggcgt tccccactcg
     4141 cggcagcagg cgtcatacaa ccagtcacgc gccgcaacaa tcagcgtctc cgccagatag
     4201 ctgagctgct gcaatatgct ctcttcagta accagtgcca gcgtttgcgc ccaggcgatg
     4261 cgcaccataa tgcgccgccg gaatagccgc agctcgcgca ttaacccggc ttcgtcactc
     4321 acattacaga gcgcctcctg caaccatgcc gcgtaatgct gccattcgtc ggcctgcggc
     4381 ggttggcttt ccagttccgt cagccactct ggatgcgcac ttatgctgtc ctgcacaaaa
     4441 tcactgaaag taagtactga cttcgcctgt gcactaagtg attcctcggc taaaggctct
     4501 ggcagccgct caacaacggt ctgccagtac tgctgtaacg gtgaagagag cggcttcata
     4561 agcgatttta tccttgcctg attaacgttt tccgctgtgc aaccagaacg gttcctgatt
     4621 gtttgcctca ttacggaaat gttcaatttc aatgcgttgc ccggtcgcaa tagcgtgacg
     4681 cagcccctgc cagttctcca gccaggcttg cgcgacgaca ggatcatagt aacccgccag
     4741 caacagtatt gagtcaatat cacgcgtcag gcgtggcaac tggtcacggt agcgatcgcc
     4801 taacggctgg cagaaaacgc ttttcagttc agcggcatgg cgggaaagat ggatatcggc
     4861 aaagcgtttg aaggaatcgc tcattttact ctgcgctttg gcatctaaaa acggctgcca
     4921 ggctttgctt accagccatt cggtcaacgc cagcttcgcc attgccgttt cggtagagta
     4981 gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct tcgcattggg tcagcaaatc
     5041 acgtaagtga gtactcgctt tacgcggcac gataccgccg aacagcatca gcgtatgacg
     5101 gaccaggcta atggctgcca gcacctgttc tttcgccgca tcgttgccgc gtacccacag
     5161 ttcttcatga tactgccatt gcgctaacgc cagctcgagc gccgcttcca gcccctgttc
     5221 cacatcggct tttgccgcaa catgcaaaat ggtggtcggt ttgatttcac gcgccggatt
     5281 gccctgcgcc agatgatagc cacgcgccgc tttgctcagg ctgccctggc gtaatccggt
     5341 ttgcgatacc agttggttcg ccagtttcag caccgcgcgc gtgtcgccgc taagcagctc
     5401 cagttccagc tcacagatag gttcagcaaa ttcccccgct ttcacctcac cctgatcgag
     5461 agcgatttca attcggcttc catcgaccgc caccagccat ttttcgcgat aaaaatcggt
     5521 gctgaacagc ggttgtacgc gggaagcgag atcggcaggc aattcgccgt tcggccagac
     5581 ttccgtcggt aactgcgcca ggtcgagcgt cggttcgctc aacgccacgt tatattccgg
     5641 gcgctggtgt aagccgcctg tcactcttcc tgcaactttc atggtcatct catagcgacc
     5701 gttttcgcca cgaatacgta agcccatatc gtgcccacgc agccagttat ccggcgtttc
     5761 gtagtaaata ttcagcaact gcacggggtc atggtgctcg ccgcccagcg tattgagatg
     5821 gtcacgcaac gcctcaacgg cactgtgatt aacaataaac tttaattcga tttcctgagc
     5881 catggccttt acttatcggt tatgtcacat aagtgacgat gaacggcgaa cttaatccga
     5941 tcttttttgt cagtagatag tattttgcgc caaattgcca tgcaacgggc aatttgacgg
     6001 gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg attccgattg atgtcgaatg
     6061 ctttgcgtag cgacactgat gccactacta tcgttccact ttccatgaca ataacgacag
     6121 cctgatgcca aaattacgcc tgatcggatt aactttactc gcacttagcg cgactgccgt
     6181 ctcacacgct gaagaaacgc gctatgtttc cgacgaactg aatacctggg tccgcagcgg
     6241 tccgggagat cattatcgcc tcgtgggcac ggttaacgcc ggcgaggaag tgaccttatt
     6301 acaaactgat gccaacacca attatgccca ggtgaaagac agctctggtc gtaccgcctg
     6361 gatcccgttg aaacaactta gcactgagcc aagcctgcgc tcccgtgtgc cagatctgga
     6421 aaatcaggtc aaaaccctga ccgataaact cagcaatatc gataacacat ggaatcaacg
     6481 caccgcagaa atgcagcaaa aagtggcgca gagcgacagc gtgatcaacg ggttaaaaga
     6541 agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag aaaaaggtcg atgccgccag
     6601 cgtacagctg gatgacaaac agcgcaccat catcatgcaa tggtttatgt atggcggtgg
     6661 cgtgctgggg cttggcttgc tgctcggtct ggtactgccg cacctgatcc caagccgcaa
     6721 acgcaaagat cgctggatga actaaatcgc cttcttcgtt gcactgtctt attattagga
     6781 attgtgtatt tgacgaaggg atgatggcgt gaagatttat ctggtcggtg gtgctgttcg
     6841 ggatgcattg ttagggctac cggtcaaaga cagagattgg gtagtggtcg gcagtacgcc
     6901 acaggagatg ctcgacgcgg gctaccagca ggtaggccgc gattttcctg tgtttctgca
     6961 tccgcaaacg catgaagagt atgcgctggc acgtaccgaa cggaaatccg gctccggtta
     7021 caccggtttt acctgctatg ccgcaccgga tgtcacgctg gaagatgatc ttaagcgtcg
     7081 cgatctgacc attaatgcgc tggcccagga cgataacggc gagattatcg acccgtacaa
     7141 cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt tcccccgctt ttggcgaaga
     7201 tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt tatgcccacc tcggttttcg
     7261 tattgccgat gaaactctgg cgttgatgcg cgagatgacc catgcgggtg aactggaaca
     7321 cctgacgcct gaacgggtgt ggaaagagac ggaaagcgcc ctcaccaccc gcaatcccca
     7381 ggtgttcttc caggtactgc gcgattgcgg tgcacttcgc gttttattcc cggaaattga
     7441 cgccctgttt ggcgttccgg cccctgccag gtggcatccg gaaatcgaca cgggtattca
     7501 taccttaatg acgctctcaa tggcggcgat gctgagtccg caggtcgatg tccgtttcgc
     7561 aactttatgc cacgatctcg gtaaaggact gacgccgcca gaactctggc cgcgtcatca
     7621 tggtcatggc ccggcgggtg ttaagttagt ggaacaacta tgccagcgtc tgcgcgtgcc
     7681 aaatgaaatt cgcgatttag ccagactggt ggctgagttt cacgatctca tccacacctt
     7741 cccaatgctg aacccaaaaa ccatcgtcaa attatttgat tccatcgatg cctggcgtaa
     7801 accgcagcgt gtcgagcaac tggcgctgac cagcgaggct gacgtgcgcg gcagaaccgg
     7861 ttttgaatca gcggactacc cgcaaggccg ttggttgcgc gaagcctggg aagtggcgca
     7921 gtcagtgccg acaaaagccg tcgttgaagc gggatttaaa ggtgtggaga ttcgcgagga
     7981 gctgacccga cggcggattg cggcggtagc cagctggaag gaacaacgtt gcccaaagcc
     8041 tgaatgagta ttgggcattt tgccggatgc ggcgtaaacg ccttatccgg ctaca
//
LOCUS       DACAPB010000190         8082 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.190, whole genome shotgun sequence.
ACCESSION   DACAPB010000190 DACAPB010000000
VERSION     DACAPB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.190"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..193)
                     /locus_tag="IT904_003933"
     CDS             complement(<1..193)
                     /locus_tag="IT904_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="acts with MukB and MukF to condense the chromosome
                     and allow for segregation during cell division; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein MukE"
                     /protein_id="HAP3193642.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVE"
     gene            complement(174..1496)
                     /gene="mukF"
                     /locus_tag="IT904_003934"
     CDS             complement(174..1496)
                     /gene="mukF"
                     /locus_tag="IT904_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAP3193643.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(1493..2278)
                     /gene="cmoM"
                     /locus_tag="IT904_003935"
     CDS             complement(1493..2278)
                     /gene="cmoM"
                     /locus_tag="IT904_003935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAP3193644.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            2414..3193
                     /gene="elyC"
                     /locus_tag="IT904_003936"
     CDS             2414..3193
                     /gene="elyC"
                     /locus_tag="IT904_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAP3193645.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(3170..4063)
                     /locus_tag="IT904_003937"
     CDS             complement(3170..4063)
                     /locus_tag="IT904_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAP3193646.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(4217..4963)
                     /gene="kdsB"
                     /locus_tag="IT904_003938"
     CDS             complement(4217..4963)
                     /gene="kdsB"
                     /locus_tag="IT904_003938"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP3193647.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(4960..5142)
                     /locus_tag="IT904_003939"
     CDS             complement(4960..5142)
                     /locus_tag="IT904_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAP3193648.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(5194..6426)
                     /gene="ycaQ"
                     /locus_tag="IT904_003940"
     CDS             complement(5194..6426)
                     /gene="ycaQ"
                     /locus_tag="IT904_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein YcaQ"
                     /protein_id="HAP3193649.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHDDLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGCC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(6463..7449)
                     /gene="lpxK"
                     /locus_tag="IT904_003941"
     CDS             complement(6463..7449)
                     /gene="lpxK"
                     /locus_tag="IT904_003941"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAP3193650.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(7446..>8082)
                     /locus_tag="IT904_003942"
     CDS             complement(7446..>8082)
                     /locus_tag="IT904_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP3193651.1"
                     /translation="VALVGRSGSGKSTIASLITRFYDIDEGEILMDGHDLREYTLASL
                     RNQVALVSQNVHLFNDTVANNIAYARTEQYSREQIEEAARMAYAMDFINKMDNGLDTV
                     IGENGVLLSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQAALDELQKNRT
                     SLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLLEHRGVYAQLHKMQFGQ"
BASE COUNT         1975 a         2251 c         1960 g         1896 t
ORIGIN      
        1 gctcaacgtt ataacgcgcg taaaactctt ccagatattc ctgaaaatcc atcaggaatg
       61 catgattatc cagttcgtcg aggccaatat ggcggcctga acgtaaggcg ctgtccagcg
      121 ccggaaataa cggattcgcc agcgcctgcg ccagcttaac cggcatcact tgttcaatat
      181 ttgtcgatga catgcgcctg taccttggct ccgtaatcat taatcggctg ccatttcgct
      241 ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta ccgcctgatc aataacaata
      301 cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca gatattcgcg taccaccaga
      361 ccaagatcca gcggcacttg tctggttttg tacacggcaa gttgttcttc gatgatcgcc
      421 gccagctgtt cgcggatctc gttaaactct tcgtattcca gatcctcagg aagttcccca
      481 gtcacttctt catcgcgcag tgccatctct tcgtcacgca tatccagcag ccgatcggca
      541 ttggcataag ttagcgccca cggctcatca aaataggttt gtaccgactg acgtaaccgc
      601 tgagcaaaga cgcggttttt atccatatcg atcgcggtac gaataaattt gtgtacgtgg
      661 cggtcgtagc caatccacaa gtcgatggat tgctggcccc aactgataat acgatcgagt
      721 ttgctctgca gatcgaacac cagacgatcg acgaaatgca gatcgtcatg ggtcatcgtc
      781 gcatcctgaa tgcgcaacag attagcctgc aatttgtcgc ctgccgcttc cagcgtatcc
      841 tgcaattcac gcagcgttcc ggaagtttcc gaaagcaaca attcacagct ggaaatcgcc
      901 gcccgccagt ctttgttcag caactgggcg atatcgtcct tcacctgctg ctgctgttcg
      961 tccatcagac gttgcgtcag gtcgatactg tcgaaaattt ctgctaccga atatttcagt
     1021 ggcgcataga cattacggtg ccagtgaaat tcatcaccgc cctcttcggc ggcatctgct
     1081 gcgcgtttga gctcacccgc cacaatcgac aactgcatag aaagacgcag cgtagaaaac
     1141 tcgcgctgac ggatgtagta gtcagtaatg ccgatgccga gcggcgtcag acggtaaatt
     1201 gcgttccctt ccgcctgctc gctggtaaag cggttcagca gacgttgacg caccatgtcg
     1261 ttgatcgcgt tattggcgcg cacgccgatg gtttcgctgg tttgctcaaa cgcatcactc
     1321 acatggcgga atgcatccac cagctcgcct tcactcatct caccatccag acgctcgccg
     1381 ttcagcgtgg caaccgccag cagaaaagag agtcggtcta ccggcagcga gatggagaag
     1441 tcattttttc tggcccaggc aaccagttcg gggactgtct gggaaaattc actcatactt
     1501 tatccttgct ctgcggtttg cgcgcggtga catgaatata acgccccagg gtaatatacg
     1561 gttcctgacg gcaataacgc gtttctaatt caagtaatgc ttcatagcag tcgcgctgct
     1621 ggtgtttctc gcgcagataa tcatgaaaca cgcgaacgcc tgtcttaccc ataatttgcc
     1681 aaccagcttc ttccagccac agataaacct gcgccgggtc gcgtggataa tctggcgaaa
     1741 gcgtccgttt tttctttttc ggcattcccg cctgcacgta atcaaaattc ccggcgacca
     1801 tgttatgcat caacaaacca tgcgcattgt agaacattaa cgacaacacg ccgcctggac
     1861 gcaacactga ccacagggtc tgcaatacgc tgcggggatc agccacccac tcgagcaccg
     1921 catggaacaa tatcagatca acgggcgttt ccaaatgcga agcaacatcc tgagcggcgc
     1981 aatgtataaa ttgcatgttg tcgctcacac cttttgcttc tgccgcctgt tttgcgcggt
     2041 cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg cccacgctcg gccattttga
     2101 ttgcggtctg cccttctcca ccgccagcat ccagcacacg cagtttttgc gggcccattt
     2161 ccgccagcac gcgatcgaga tcctgccaca gaatagcctg tcgaagctgc cctttggtgg
     2221 tgccgtaaat gttacgggaa aacttttccg caatatcatc aaaattgcga tcctgcattg
     2281 ctttctccac cggttggcta agacgttttc tgataaaggc gctattttgt cacagccacg
     2341 gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc cttgctatat ggttaaaaaa
     2401 ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt ggcaatatgt tgctgccact
     2461 tccgctgatg ttgttgatta ttggcgctgg cctggcgctg ctgtggttta gccgttttca
     2521 aaaaaccggg aagattttta ttagcattgg gtggctggcg ctcttgttat tgagtttgca
     2581 accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc tatccgacat ggaataattc
     2641 gcaaaaagtg gactatatcg tggtgctcgg aggtggttat acctggaacc cgcagtgggc
     2701 accgagctct aatttaatca ataacagtct gccacgcctg aatgaaggta ttcgcttatg
     2761 gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc gtagcaaaaa ccaatacggt
     2821 gagtacagcg gaagtaggtg ccagagttgc gcaatcgctg ggcgtgccgc gcgagcaaat
     2881 tatcaccctg gatttgccaa aagataccga agaagaagct gcagcagtga aacaggcgat
     2941 tggtgacgcc cctttcctgc tggtgacctc cgcatcacat ctgccgcggg cgatgatctt
     3001 ttttcagcag gaagggttaa atccgctacc agccccggcg aatcagttgg ccatcgactc
     3061 gccgcttaac ccgtgggaac gggcaatccc ttctccggtc tggttgatgc atagcgatcg
     3121 tgttggctat gaaacgcttg gccgtatctg gcagtggcta aaaggttcgt caggcgagcc
     3181 acggcaagag tgattttgat gccagatcga agttgcgccg actaaatcgt ccggtattaa
     3241 ccagttgcgc aacttcatcc cataacacat acaaccaacg ccgccagatg aacgactccg
     3301 ccactggcgc gcgttgcagg taactccaga gcaaatcttc cgccagagaa ttatccatta
     3361 gtcggaacag ttcgtattct cgcggtgccc aaagcattag tcccggcccg accatcgcca
     3421 gtaactgatc gctgcgcgaa tctttcaaca tgctgcgtaa acagaagtta ccgtgaatca
     3481 gcacacaatt gtcgttaaag ccttcaaata atgccgggag acattcgcga gtgcgaaaca
     3541 ggatccgctt atcctgcatc gtcagaccgg tgttattgaa ctgattgagc gtggtccata
     3601 gcacttcaac atgttgccgg taccatgagg gccagaaatt ttcctgagta ttgtcgaccg
     3661 cgccgacgca accgcgactg tcctgacggt gccaggccag taaggcttca acgatttggt
     3721 ctttgagttg ttcccagcgt tctggtgttc gggctggtgc ctccaccgaa acaccacgca
     3781 tccgctccag tagcaggaca tcggggccgg ggtgttcttc atgcgtcatc acaccataaa
     3841 tagtcggcat acggacagtc ccacttcttg ccagcatggt ggttttccat gccagttgtc
     3901 gggcttttcc gggcgtacta aagcttcttg ccattaacgg cattgggttt ccctggctgt
     3961 catacaaagc ccacaacgcc gtatccgctt tttcattgac gcactcaata cgactgagtt
     4021 tttcgcccag taaatggctt aattcggcac gcagctgttc cattcagatt accctcataa
     4081 aagctactgc tttcataatg agcgccaata taaaagatgt cacctgaatg agatcaattg
     4141 ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac tcctcccaaa attggctgaa
     4201 gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg ctcaagatct tcaggggtat
     4261 ccacacctgt gccaggaact tcctgagcaa cagcaacatg gattttttcg ccgtaccaca
     4321 gaacacgaag ctgctctaac atttcgatgt gttctaacgg acttggctgc cagttgacgt
     4381 aacgacggat aaagcctgca cggtagccat aaataccaag atgacgcagg aagttatcgc
     4441 caacggtttc aaggccttct gcaaaacgat cacgatccca aggaatggtg gcgcgagaga
     4501 agtacagtgc atacccttca gcgtcgagaa ccactttcac cgcattcggg ttaaacgctt
     4561 cttccgcatt gtggattggc accgccagag tcgccatacc cacctgacgc tgagcgaggt
     4621 tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg ttcatcaccc tgcacattaa
     4681 cgatcaccgt gtcgtcgctg aatgcgcatt tttcgacaac ttccgccaga cgttctgttc
     4741 ctgactgatg atcggcgcgc gtcatacata cttcaccgcc agcggcttca acggcgcggg
     4801 caacatcctc atgatcggtt gccacgatga tgcgctcggc acctgattca cgcgcgcgtt
     4861 caagaacatg aacaatcatg ggtttgccgt taatatcaac caatggttta ccgggcagac
     4921 gcgtggacgc gtagcgcgcg ggaataatga ccacaaaact catgatttac tctcatcagc
     4981 agtcagcacg cgggcttccg tttccagcaa cactggaatg ccatcacgca gcgggaaagc
     5041 gaggttgtcc agtttgcaaa taagctcttg tttttcctgg ttataccaaa gttttccgtt
     5101 gcaaaccggg caggcaatga tttcaagcag acgatgatcc atagttcctc cagatggatc
     5161 gggttatgaa tgcataaatc ttatcataat catttatgcg acggggtcta tttcccagcc
     5221 ggtgcggcaa tcagtaaaga ggccttgcgg gcagcatcct aatgtcaccc gcgttgcctg
     5281 ctgccagtta gcgaaatcag taatcgcctg acgtagccct ttttgcagcg ttgtcgttgg
     5341 tttaatacct tcctgtaacc acagagagat aacttcaagg atgcctgtct ggcgatgcat
     5401 tttggcatcc attcgcccaa ctaattgccc acgatgtaat aacggcagaa caaaatagcc
     5461 atactggcgt ttcggcgctg gggtatagca ctccagccgg tagctaaaat caaaaagctg
     5521 ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc gaaagtaccg cgctgtgcgt
     5581 tgcagtgagc tttccggcca gcgctcgctc gagtagcggc agcaaatcat catgcagcca
     5641 aagattgccc aatttttcaa catgcacagc aatgatttgc tgctgttcag ccctcgcttc
     5701 gcgccacgcc gccagtgccg gacgtttcag ccgatagtaa tctgccagcc actgttcgcg
     5761 gaatattccc aggctacgcg cactgttatc cagcatgatg atttctgctt ctgtttgcga
     5821 aacgagatcg cgctcatcat cccagtcagg catgacacgg tgggttaaat cataaacgcg
     5881 ctggaagttg cgccgttcaa tcaccatcac ctttccggca gtaaataaac cttccagatg
     5941 ccgtttatgc ggcttccatt cccaccagcc gcttgcacct ttacgaggat gctcaaaatc
     6001 ggctgaacgt accggcccct tatcatgaat atgctgaatt aactgtgcaa tttccgcctc
     6061 atgttcctgc atccaggcgt ctttgtattt ccagcccatt ttttcaggtg ccagcatgcg
     6121 gtggcgaata agacgaaagt cgctacgcgg cataaagcag gcttcatgcg cccagtattc
     6181 cattaattcg ccacgcgcca gagactcatc cagccactgg gcaggataat ttcccagacg
     6241 actgaaaagc accagatatg gactacgggc aacaatattg atggtatcga tttgcagcaa
     6301 ggacatgcgg gagatcgttg ccggaatatc ctccaacgac gctcgacggc ggggtttgtt
     6361 taacaggcct tgtgcggcaa ggtgaagatt acgcgcatca gcaagggaga ggtgcggcag
     6421 cgacattcat gactccatca atcgaacgct gccgcggcgt aactagttgc cagaagccag
     6481 cgaggttagt tgcgtaagca gtttcgctgg ttcatcacct gaaagctgtg cgtctacagg
     6541 caaataccac caattttctt ctgcaaaggc ccggcatttc accgcatctt tttcagtcat
     6601 taccagcgtt tgcccggcgc ttaccaacgc actgacatcc gcatggttca aagactgatg
     6661 atcggccagc ggtacacatt tttccggttg tacgccacac atcttcagcg tggcaaaaaa
     6721 gcgcggcgga tgcccaatcc ccgccatcgc cactacatgt tcaagctgag caacgtcaca
     6781 acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc agatgcatgg ggatttcacc
     6841 gctgcgaggg acaccgccgt tgacgattac cgcatcaacc gactttaagc gccccgctcg
     6901 ctcacgcatt ggccccgccg gcaaccacca gccattgcca aagcgacgca caccatcaat
     6961 aacgacaatt tccacatcac gcgccagacg gtaatgctgt aaaccgtcgt cggttacgat
     7021 gatctgcaca tcagggtgtt gcgccagaat ggcttttacc gcatcagaac gaacgggaga
     7081 aaccgcaaca ggcgcatcag tgcgttgata aatcaacaca ggttcatcac ccgcctgtgc
     7141 tgttgtggta tctgccgaca ataacagcgg ataagattca gccttaccac catatccccg
     7201 cgatacgacc cccacgcgaa taccgcgctg ttgcaactgt tccaccagcc agacaacgac
     7261 cggggttttt ccgttgccgc ctgcggtgag attaccaacc acgacaaccg gtacgggggc
     7321 acgccaggcg cgcttcagtt ttagtttata gcaaagacgg atcgcgccac tcaccaggcc
     7381 atacaaccag gagagtggca gcaatagccg ccacaaaggg gattcaccag accagatttt
     7441 ttcgatcatt ggccaaactg cattttgtga agttgcgcgt aaacgccgcg gtgctcaagc
     7501 aaatcgttat gcgtaccgcg ttccacaatg acaccatcct cgacgaccac gatttcgtct
     7561 gccttttcaa tggtagacaa gcggtgggca atcaccagag aggtacggtt tttctgcaac
     7621 tcatccagtg ccgcctgaat cgcacgttcg gattcggtat ccagagccga ggtagcttcg
     7681 tccagaatca gaatcgggct atcacgcaac aaggctcgag cgatagcaat acgctgacgc
     7741 tgaccgccag agagcagcac gccgttttca ccaatcactg tatcgagacc gttatccatc
     7801 ttattgatga agtccatggc gtaggccata cgcgccgctt cttcaatttg ctcacggctg
     7861 tactgttcag tccgtgcgta agcaatgttg ttagcaaccg tatcgttaaa cagatggaca
     7921 ttctgcgaca ccagagcaac ctggttacgt aacgacgcca gggtatactc gcgcagatcg
     7981 tgaccatcca tcaggatttc gccttcatca atatcgtaaa aacgcgtgat caggctggcg
     8041 atggttgatt tacccgaacc agagcgtcca accagagcaa cc
//
LOCUS       DACAPB010000191         8020 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.191, whole genome shotgun sequence.
ACCESSION   DACAPB010000191 DACAPB010000000
VERSION     DACAPB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.191"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..687
                     /gene="dinG"
                     /locus_tag="IT904_003943"
     CDS             <1..687
                     /gene="dinG"
                     /locus_tag="IT904_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="helicase involved in DNA repair and perhaps also
                     replication; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAP3193652.1"
                     /translation="DSPFNHCEQGKIVIPRMRVEPSIDNEEQHIAEMAAFFRKQVESK
                     KHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGDQPRYRLVELHRKRVANGERSVLVG
                     LQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVITEGEWLKSLNRYPFEVQSLPSASF
                     NLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKRLLDALPVFPIEQPEVPEGIVKKKE
                     KTKSPRRRRR"
     gene            715..1677
                     /gene="ybiB"
                     /locus_tag="IT904_003944"
     CDS             715..1677
                     /gene="ybiB"
                     /locus_tag="IT904_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAP3193653.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            1818..2903
                     /locus_tag="IT904_003945"
     CDS             1818..2903
                     /locus_tag="IT904_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAP3193654.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(3132..3392)
                     /locus_tag="IT904_003946"
     CDS             complement(3132..3392)
                     /locus_tag="IT904_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP3193655.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3657..3923)
                     /gene="ybiI"
                     /locus_tag="IT904_003947"
     CDS             complement(3657..3923)
                     /gene="ybiI"
                     /locus_tag="IT904_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAP3193656.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(3997..4674)
                     /gene="ybiX"
                     /locus_tag="IT904_003948"
     CDS             complement(3997..4674)
                     /gene="ybiX"
                     /locus_tag="IT904_003948"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAP3193657.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(4716..6998)
                     /locus_tag="IT904_003949"
     CDS             complement(4716..6998)
                     /locus_tag="IT904_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HAP3193658.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(7263..7523)
                     /locus_tag="IT904_003950"
     CDS             complement(7263..7523)
                     /locus_tag="IT904_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP3193659.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7799..>8020
                     /locus_tag="IT904_003951"
     CDS             7799..>8020
                     /locus_tag="IT904_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmF-related methyltransferase"
                     /protein_id="HAP3193660.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDG"
BASE COUNT         1864 a         1991 c         2194 g         1971 t
ORIGIN      
        1 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgcgttgag
       61 ccttccatcg acaacgaaga gcagcatatt gccgaaatgg cggccttttt ccgtaagcag
      121 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
      181 cgctttctcg actatgtgac ggatttacgt ctgatgttgc tggttcaggg cgatcagccg
      241 cgttaccgtt tagttgaact gcaccgcaaa cgcgtcgcca acggtgagcg cagcgtgctg
      301 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
      361 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
      421 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
      481 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
      541 atctacgaca aacgcttgct gaccaaaaat tatggcaagc gactactgga tgcattaccg
      601 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
      661 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
      721 tatcgcaaaa tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
      781 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
      841 ggcggcgtat tgattgcgct gcgtatcaaa ggagaagggg aagcagagat gctcggcttt
      901 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc cagcaggcaa gccgatgccg
      961 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg
     1021 attctcctgc acaaactcgg ttttcctgtg gtggttcacg gggttagcga agatccaacc
     1081 cgcgtgctga ctgaaaccat ttttgaattg atgggtatta cgccaacgtt gcacggcggg
     1141 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgccagtcgg cgcgttttgc
     1201 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
     1261 accctggcga aactggcgac gccatttgcc gaaggtgagg cgctgcgtct ttccagcgtt
     1321 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggcgggcg
     1381 ctactaatgc atggtactga aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc
     1441 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggacac tgctggtagc
     1501 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggattga gcgttgcctt
     1561 gctggcagcg aaccgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
     1621 acgggtgaag cggcaactat cagcgacggc ctggcgcgcg ttaatcaggc attttaattc
     1681 ttttctcagc cggatgagcc atgtcatccg gcttttaccc caccgcaata tgaaattcct
     1741 gcatctttat tgaccttccc acgcccggcg tgcagcataa aaatacaaca aacacataac
     1801 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
     1861 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
     1921 atcatttaat cgcggcaaac ctggcagggc atgattcaca tggtattggc atgatcccaa
     1981 gctatgtacg ctcctggagt caggggcacc tgcaaattaa ccatcatgcc aaaaccgtta
     2041 aagaggcggg ggcggcggtc acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac
     2101 atgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgcc gccgtggcgc
     2161 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg
     2221 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
     2281 gtcgcgacag ccgctttggc accaatccgt tctgtgtggt tttccctcgt aaagataatt
     2341 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
     2401 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
     2461 ccaatccggc ggtaatgcag gagtcgccgt tgggttcgct gttgaccttt gccgaacata
     2521 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttcc ggcggtaaaa
     2581 cgacgcatca ggaaacgtta caaaccagtc ccgatgccat tcttaactgc atgaccacta
     2641 tcatcatcaa cccggaactc ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
     2701 ccgagtgggt gaaagcctcg ccgcatgatg atgataagcc gattttgcta ccgggcgagt
     2761 gggaagtgaa cacgcgtcgc gaacggcaga agcaggggat tccactggat gcgggaagct
     2821 ggcaggccat ttgtgatgca gcgcggcaga ttggtatgcc ggaagagacg ttgcaggctt
     2881 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
     2941 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
     3001 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
     3061 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcgtaa acgccttatc
     3121 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
     3181 gaagtgatgc tgtatccact ggcaccggct gccgcggctt tctcagccag tttcgcttcc
     3241 agggcatcga gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
     3301 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
     3361 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
     3421 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
     3481 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacctact gataaaccat
     3541 ttatttgttg tacttatgaa cttgttttat accgcgtggc agtcacagca gcgttaaagg
     3601 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
     3661 cgtaactggc tgtctttcga acctctgcga ttatatcctg tataagctgg tttttgtaaa
     3721 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggcc
     3781 tgcgggatgg gggcaccgca ctcttcacat tcatccaggc tttcgccgcg cggaatttca
     3841 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
     3901 tcgttagccc aaccggatgc catatcgacc tccccatatc aatacttgta cagttaagtg
     3961 tagctaatcc agggacgaac tcgggcagtt caagcatcag atctccgacc attcccgcag
     4021 cagattatga taaagattaa gcagcgacag gatctcttca ctttcgccgt agcggctttt
     4081 cagcgactga atattgttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
     4141 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
     4201 atgcaggctg ctggagggat acaacacgag atcgcctgcc gggagtttta cccgatgttg
     4261 tccgaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
     4321 cagcgtggca gaaaggtcag tacgcatcca gccgttttgt ggatggctgc gtactgcgcc
     4381 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
     4441 ggtacgcggc aaggccgcgg caaagaataa agcatgttgg ttaaccgcgt tcagcacctc
     4501 attttgcagg gcggcgtata acgtgctgcg ggtgtcgacc tgttgattgt tcttaacttg
     4561 cgcaccttgt gcgccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc
     4621 gcgaaaacga gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaatt
     4681 tctccaaaaa gtggggcctg cgccccacat ctgattcaga aatgcatatt ggctgtgagc
     4741 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
     4801 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaactcga
     4861 taccccagtt tggcatcggc gacccagtaa ccttcggtaa acgctggcgt tcccaccgcg
     4921 ccgtctgaac ctttatgcat actgccgata tagcgtgcgc ccgcgccaac agagatatcg
     4981 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
     5041 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag
     5101 ccgccaatca cctgccacgc gggagtgata ttcccggcca cggatatctc atagccttcg
     5161 acgcgtttct taccgtattg cgagtaagtt ccgtcatcat tttgctcaac ttcattttcg
     5221 atatcagtgc ggaacagcgc ggcggtgagc aacagacgtt tatccagaac ctgccatttg
     5281 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
     5341 ccgctgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
     5401 tagttaatat agacattgcc gttttccgtc aggtgataca gcgccccggc tttccagttc
     5461 atcagattgc ccgacttggc ggtgtcgacg gtggtgaccg gagaaccttt tgccacacca
     5521 gttgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca
     5581 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
     5641 gtatcaaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
     5701 gtcaggccgc cgggatgaat gctgctgtca ggatgataaa tatttaccgc gggtaacgtc
     5761 accggattaa cgccgtagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca
     5821 tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt
     5881 ttattactca catctttggt attcgccgtg cgtgaccagg tccagctatt cacatcgctg
     5941 gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt
     6001 acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa
     6061 cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta
     6121 tgagtatcaa cttttccgga atgattcagg gccgccgttc ccgcagatgg ggcagaatag
     6181 cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgggt gacatgcaga
     6241 taattaagat acaaacgatt cgctgtacca aggccaaaag cgacagaagg ggcgacgccg
     6301 taacgctcat ttttgacttt gtcgcgtccg gcatcgtgcg ttttttcgcc cattacattc
     6361 aggcgcaccg cggtggtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac
     6421 caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg
     6481 atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta
     6541 atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaatg
     6601 ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg
     6661 gtggagttac cgttctcacc cgcaaaaaac gcacccacgc cggggacgtt tttgagcgcg
     6721 tcggtaaggt tggttgcgcc ctgatcttta atcacttgtt cagaaattac cgtcatcgtg
     6781 cgggtagtat ccgctaccgg acgcgagaat ttcggatcgg cagattgttg tggcgcataa
     6841 agcgaaggcg ttgatgcttc gacaaccagc gtgtcatccg cgttagtttg cccttcggcg
     6901 gcgagtgcct gagccacagg cgtgatgcca atacaaagac cggcaaagaa cgtgagcgaa
     6961 tgaaattgtc tggcagggaa attgcgattg ttttccattt tgcaggtgac tttttcttat
     7021 atgttgaaat tttcactgcc acgagaaagc atctctgcgg atgcgatgcc actgttaatc
     7081 ccgtgatagt gttcgtgcaa gtgataataa ttttgataag cattatcact tggagcgatt
     7141 ttctatcacc cattgcgcga aaaagaaata catttattta cattgagttc atatttgatg
     7201 ttttttgatt acaaaacaga cgaaaactgc atccagaggc gaatgcagtt acgttggtgg
     7261 ggttatttgt agatggttgc agtaccgaac atctggtcat ttccgcctgc ggaattaatg
     7321 acataacctt tcgcgccttg ttcgcgcgct ttttctgcca gtttatcctc cagatcgctt
     7381 aggtttgatg cgccagttgc cgaaacggta ccggcgggcc gtagctgacc gctgtccagg
     7441 ttactcatcg gttgcgcggc ataagccgtg agagagattc cgctcaggac ggtggcaatc
     7501 agtagtgtga ggcacttttt cataatgaca tcctcataaa tacaacgggt agtgcctgat
     7561 aagtgtaggt cgcctgcgcg atgcaaatga cgcaaaaaat tgacagagat gtgcttattt
     7621 tttgaacgat atatttttac aaaataagac aaatcaatga catgcataat gctgattttt
     7681 atgatgaatt gccataaacc atagccatgt taaggttttg gggaatgata aacgcagcca
     7741 gatatttacc ctgtcgcgtt atgcgggtat cttacgccgc tgcttaaagg agaatgctat
     7801 gtccgcccag aaaccggggt tgcatccgcg caaccgtcat cacagccgct acgatctcgc
     7861 cacgctttgt caggtcaatc ctgaactcag gcaattcctc acgcttacac ccgccgggga
     7921 gcaaagcgta gactttgcca atccgctggc ggtgaaggcg ctcaataagg cgttgctggc
     7981 ccatttttac gccgtagcga actgggatat ccccgacggt
//
LOCUS       DACAPB010000192         7950 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.192, whole genome shotgun sequence.
ACCESSION   DACAPB010000192 DACAPB010000000
VERSION     DACAPB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.192"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..262)
                     /locus_tag="IT904_003952"
     CDS             complement(<1..262)
                     /locus_tag="IT904_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP3193661.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEILKKA"
     gene            complement(332..679)
                     /locus_tag="IT904_003953"
     CDS             complement(332..679)
                     /locus_tag="IT904_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP3193662.1"
                     /translation="MFKSLLISKMDGRHSAQIAEIAESELPQGDVEVNVDCSSINYKD
                     ALAITGRGPIVRHFPMVPGIDFAGTVSASSHPEYQTGDKVLLTGWGVGEKHWGGLSEK
                     ARVKGDWLTPLPA"
     gene            complement(1315..1990)
                     /locus_tag="IT904_003954"
                     /pseudo
     CDS             complement(1315..1990)
                     /locus_tag="IT904_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2243..3712
                     /locus_tag="IT904_003955"
     CDS             2243..3712
                     /locus_tag="IT904_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3193663.1"
                     /translation="MHIDSTEKNNKQKLGLLALTSIVVSSMIGSGVDGLPQNMAENSA
                     VGPVAIAWIVCGIGMFFISRTFIVLSNIRPDLQSGVYMYAREGFGRFTAFNVAWGYWL
                     MTIFSNVAFAIMVMDTLNYFLPGDFKGGNNIPSVIGASLLIWGFNFLVLSGTRIAGSI
                     NIVGTLAKLIPLVAFIIILMLFMKYTTFADNVWGHSASKASQDLGSIFSQVTSPLSVA
                     LWSFIGVEGAVALSGRAKNKRDVGKATFIGFVVSLIICLLVSILPFGVLTQHELSAIP
                     TPSTAGILKIVVGEWGKIMIILGVLISVLTSWLAWTIICAELPMVAAQNGTFPKPFSK
                     KNVKGSASVSLYISSAFMQIVVLLVYFSNDAWLAMLAISTLTVLPAYLMTTAYLFKLC
                     ISGEYASYTTNHSRSAALVSSIIGFVFCLFMFYASEIKYLAMVPVLLTLGVPLFIWAR
                     KKDDRADKIFTLRELAFFILLLVLDIIVFILLHYKTIHF"
     gene            3817..6186
                     /locus_tag="IT904_003956"
     CDS             3817..6186
                     /locus_tag="IT904_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAP3193664.1"
                     /translation="MKFNHNLLFISSQYLDGDNPSQQVLEELQTELAERGFKIHITHQ
                     ISDGLKIIEKSPQYSGIGFYWEPDNPTFAEELQHFISIFRKRNATTPLIIFSEQNITD
                     RIPLDVLKEVSEYVYLFSESAAFTANRLYSLVHRYADKLLPPYFKTLKDFTEDGDYYW
                     DCPGHMGGMAYLKHPVGIEFINFFGENMMRADIGVATAEMGDYLIHAGPPKKSEEIAA
                     RLFGSDWTFYGVSGSSGSNRIVAQAAVGADEIAIIDRNCHKSLNHGLTLSQARPVYLK
                     PTRNAWGLIGPIPTGRLKKASIDALVANSRLASGAVSQSPSYAVVTNCTYDGFCYNVN
                     DVVRHLGESAPRIHFDEAWYAYARFHPLYQSRYAMDAEETPNRPTLFAVQSTHKMLPS
                     LSMASMIHVKKSDRAPLNFDDFNDAFMMHGTTSPYYPIIASIDVAVSMMEGESGYSLV
                     QESIEEAIAFRKAVVSVKRQLQEQEGGDAWFFDVLQPTEVQDSDSGQRYSFEEAPVSL
                     LSHSADCWSLRSGERWHGFADDDLVETNSMLDPVKVTLTCPGIGPKGEYQKNGIPGYL
                     LTRFLDDRRIEIARTGDYTVLILFSVGITKGKWGTLIESLLAFKKHYDNDDLATDAIP
                     SLKAHSPHYDTLTLKELCQIMHEKMDELELMSHINDAVNTDPEPVMTPAEAYQKVVRY
                     KTEHIRLDDFSGRIAASMLVPYPPGIPVLMPGERMPQGNKGIIGYLRALQEFDKQFPG
                     FEHEIQGVNVDENGDFWVRAIVEEERDGQSLPGHITFKRQVSGIKKGRQ"
     gene            6318..7760
                     /locus_tag="IT904_003957"
     CDS             6318..7760
                     /locus_tag="IT904_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3193665.1"
                     /translation="MGLISLIAIVVSSMLGGGVFSLPQNTAASSALGPVIIAWIIAGI
                     GIYFIANSFRVLSDLRPDLQAGVYMYAQEGFGSFIGFNVAWGYWLMTAFGNVAFAVIL
                     MDAFNQFMPGVFTDGNNLNSIICGSVLIWGYNFLVLSGTKVAGFVNTLGTIAKLVPLI
                     LFVLLLGVLIDYSDLFKNFWGESPAILSGNGNDAAPVSLLSQILAPMTVTLWAFIGVE
                     GAVVLSGRAKNKQDVGKATLIGFLLALIIYMLMSILPFGVMPQAELAQVSNPSTAGVL
                     AHVVGQWGDWLMSAGLIISVLAGWLAWTMLCAEIPMVAGQKGAFPRAFARTNSKQAAN
                     VSLWVSSFVMQAAMLLVYFSNNAWTTMYNISALMVVPAYITTTLYLTSICRNGKYDQY
                     AKKGRRLALSSGVLGSLFCLFILYASQIEYVALIPILLTCGLPVFIWSRKEKNDGQPI
                     FTKKELIYLIMLLLCDIIVVALFIGGYLKV"
BASE COUNT         1939 a         1914 c         1922 g         2175 t
ORIGIN      
        1 tagccttttt taatatttca ttctccattt caatgcgttg tagctttttc ctcagcttac
       61 gtatttcgat ttgttctggt gttatcggag aggcttttgg tgttttgccc tgacgctcat
      121 cacgcagttg tttgacccat cttgtcattg tggaaaggcc aacatccata gctttggcgg
      181 catctgccac cgtgtatttc tggtcaacaa ccagttgagc ggattcgcgt ttaaactctg
      241 cgctaaaatt tctttttttc attggagcac ctgtgttgtt ctgaggtgag catatcacct
      301 ctgttcaggt ggccaaattc agtgtgccac ttcaagccgg gagcggcgtc agccagtctc
      361 cctttacccg cgctttttcg gaaagcccac cccaatgttt ttctcctaca ccccagccgg
      421 tcagcaggac cttatccccg gtctgatatt caggatgtga gctggcggac actgtaccgg
      481 caaaatcaat gcctggcacc attggaaagt gccggacgat aggcccgcgg cccgtaatag
      541 ccagtgcgtc tttgtagttg atagatgaac agtcgacatt gacctccaca tccccctgtg
      601 gcagctcgct ttcagcgatt tcagcaattt gggcactgtg gcgaccatcc atcttactga
      661 ttaataagga tttaaacatt tttttctcgt ctttctggtt tgatgtcatt gagctataaa
      721 gtagtcaata aacaatattg accgatcgtc taataatcaa cgctgatatc ccagtgtttg
      781 tcagaaaaca agaattgctt ttcttttcat caggttaaat ctatctttta tgatttatat
      841 caaaccagat tcggtcttca attatatagg catcaatgtt agcggtccta caagatacgc
      901 agtcgatgct tcagggcgta ctgatgaaat aaaacaagta ggctgagatt agttggtctg
      961 ctcatcgctc acagcggacc ttcagcttcg ttagccaagc gctgacatag ttaccgatgt
     1021 agcgtgttaa tctgtcggga gcatagccga aatcacttaa tccattagac ttcacctaaa
     1081 actgcaaaaa ttaccgtctt tgtcacactc accgagttta atcaaactaa cactgtacat
     1141 agtgcgaaca agagccaacc agaaagactg ttcagcatca acagtgaagt ttttccccgc
     1201 cacttccccg caattcctcc gcagttttcc cactagagag ttcgacacat cccttgcata
     1261 ccaacgcttt gattcaaatc tcggtagtgc atccaattag tagaatatgt gtttttagat
     1321 aaatgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga ttgtttacga
     1381 gccaaccgct taatgtgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
     1441 attttgccgg tcagatgctt attcttcggc accacccggc catagctgtc ccagtcatcg
     1501 ctggtgagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
     1561 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
     1621 cagagccagt gtagccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
     1681 ataagcgcca catcagcatg ggcaacaggc gaagaagtta tttgcgttgg cgcgagtttt
     1741 ttaaagtccg gatgatggtg ttaataccaa ttttcagtgt cctggcggta tcgcgaaccc
     1801 cggcaccatt aaaggccatt tcagtgatca tctctttcat acccggctta cgtgcttgat
     1861 aagtataagt gagctgaaac acacggtggc agtcacggca gcgaaatctg tcacggcctt
     1921 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
     1981 cgctggccat gagataacct caaaagcccg tattatacat cagattcaac caattagagg
     2041 catcaccctt tcctacattc gtaggacttt gattacatat agcaagggtt aattcttata
     2101 tacatagaat aaattaggaa tataatgaat tttatcgtga cgaccttatt tataattaat
     2161 ttatatcgtc agagaaatta gtgtccacaa catgaagcct tacaaagaga ttctatgatt
     2221 taaaaaaaat aagagccagc ttatgcatat cgattcgact gaaaaaaata ataaacaaaa
     2281 gttgggcttg cttgcgctaa catccattgt cgtgagctcg atgatcggca gcggcgttga
     2341 cggactcccc cagaatatgg cggagaattc tgccgtgggc ccggtggcga tagcatggat
     2401 agtctgtggt atcggcatgt ttttcatctc cagaactttt atcgtgctgt caaacattag
     2461 acctgatctc cagtcaggag tttatatgta tgcccgggaa gggttcggcc gatttacggc
     2521 atttaacgtc gcctggggct actggctgat gactatattc agcaacgtcg cctttgccat
     2581 tatggtgatg gatacgctga attatttctt accaggtgat ttcaagggag ggaacaatat
     2641 tccttccgtt atcggtgcct ctttgctgat atggggattc aactttttag tcctctccgg
     2701 tacccgcatt gccgggtcga ttaacatcgt cggcacgctg gccaaactga tcccgctggt
     2761 ggcctttatt attattctca tgctgtttat gaagtacacc acgttcgcag ataacgtctg
     2821 ggggcacagc gcgagtaagg ccagtcagga cctaggctct attttctcac aggtaacttc
     2881 accgcttagc gtggcactct ggagcttcat cggcgttgag ggggctgtcg ctctttccgg
     2941 tcgggccaaa aataaacgcg acgtgggtaa agccacattc attggcttcg ttgtatcact
     3001 gattatctgt ctgctggtct ccatcctgcc gtttggcgtg ctgactcaac atgagctcag
     3061 cgcaattcca accccatcca ccgccggtat tctgaagatt gttgtcggtg aatgggggaa
     3121 gattatgatt atcttggggg tgctgatttc ggtgcttacc agctggctgg cgtggaccat
     3181 catctgcgcc gaactgccta tggtggccgc acagaacggc acattcccga aaccgttcag
     3241 caaaaaaaat gtgaagggtt cggcctcggt ttcgctttat ataagcagtg cgtttatgca
     3301 aatcgtcgta cttctggtct acttttctaa cgatgcctgg cttgcaatgc tggctatctc
     3361 taccctgacc gttttacctg cgtatttaat gaccacggca tacctgttta aactatgtat
     3421 tagcggcgag tatgccagct ataccacgaa tcacagccgc agcgccgcgc tggtcagcag
     3481 tattatcggc tttgtattct gcctgtttat gttctacgcc agtgaaatca aatatctggc
     3541 gatggttccg gtgctgctta cgctgggcgt gcccctgttt atctgggcaa gaaaaaaaga
     3601 cgatcgcgca gataaaatat ttacgctacg cgaactggca ttttttattt tactgctggt
     3661 cctcgacatc attgttttta ttctactgca ttataaaacg atacattttt agtccggatt
     3721 aattcacagc aagttaaggt gacgataatg tctgtttctt gggcatgaca tcctcacagt
     3781 taaatactat ctgaacagta acagtggaga cacctaatga aatttaacca caatctttta
     3841 tttatttcct cacaatatct tgatggcgac aacccttcgc agcaggtact ggaagagctt
     3901 caaaccgaac tcgcggaacg cggttttaaa attcatatca cgcaccagat ctctgatgga
     3961 ttaaaaatca tcgaaaaaag cccccaatac agcgggattg gtttttactg ggagccagac
     4021 aatccgacct ttgcagaaga gctacagcac tttatcagca tattccgcaa aagaaatgcc
     4081 acgacgccgc tgataatttt ctcagaacag aatattaccg atcgtatccc actcgacgtc
     4141 ttaaaagagg tcagcgaata tgtttatctg ttctctgaat ccgcagcctt cactgccaat
     4201 cgactgtact ccttagtcca ccgctatgcg gataagcttc ttcctcccta ctttaaaacc
     4261 cttaaggact ttaccgagga cggtgactat tactgggact gcccggggca tatgggggga
     4321 atggcctatc tgaaacatcc tgttggcatt gagttcatta acttctttgg cgaaaacatg
     4381 atgcgcgctg atattggcgt ggctaccgcg gagatggggg attatctgat ccacgccgga
     4441 ccgcctaaaa aatcggaaga aattgcggcc cgactgtttg gctctgattg gaccttctac
     4501 ggtgtttccg gctcatcggg gtcgaaccgt attgtggccc aggctgcggt tggcgcagat
     4561 gaaattgcca ttattgaccg aaattgccac aagtcactta atcatggcct gaccttgtct
     4621 caggcaaggc cggtttacct gaaacctacc cgcaacgcgt gggggctgat tgggcctatc
     4681 ccgaccggac gcctgaagaa ggccagtatt gatgcgctgg tggcaaacag ccgactggca
     4741 tctggtgcgg tcagccagtc cccgtcctac gccgtggtaa ccaactgcac ctatgatggc
     4801 ttctgttata acgtgaacga tgtagtcaga cacctgggtg aaagcgcacc gcgcatccat
     4861 tttgatgaag catggtatgc ctatgcgcgt ttccatccgc tataccagag tcgctatgca
     4921 atggacgcgg aggaaacgcc aaatcgtcca accctattcg ccgtacagtc cacccataaa
     4981 atgctgccct cactctccat ggcctcaatg attcatgtga agaaaagcga tcgcgcgccg
     5041 cttaatttcg atgactttaa cgatgccttt atgatgcacg gcacgacctc accctactac
     5101 ccgatcattg catcaattga tgtggcagta agcatgatgg agggtgaatc cggttattcg
     5161 cttgtgcagg agtcaattga agaagcgatt gccttccgta aagccgtggt cagcgtcaag
     5221 cgccagctcc aggagcagga aggtggtgac gcctggttct tcgatgtgct tcagccaacc
     5281 gaagtgcagg acagcgattc tgggcagcga tacagttttg aagaggcacc cgtatcgctg
     5341 ctgagccaca gcgccgactg ctggtcgctg cgctcaggtg agcgctggca cggttttgcc
     5401 gatgatgatc tggtcgaaac caacagtatg ctcgatccag taaaagtgac cctgacttgt
     5461 ccagggatcg gcccgaaggg agaataccag aaaaacggta ttccgggata tttgctgacc
     5521 cgttttctcg atgaccgtcg cattgaaatt gcccgtactg gtgactacac cgtgctgatc
     5581 ctgttctccg tcggcatcac caaaggcaaa tggggaactc tgattgaaag cctgctggcc
     5641 tttaaaaagc attacgacaa tgacgatctg gccacagatg ctatcccgtc gctgaaagcg
     5701 cattctccac actacgacac gctaaccctc aaggagctat gtcagataat gcatgaaaaa
     5761 atggatgagc tggagctgat gtcgcatatc aacgacgcgg taaacaccga tccggaacca
     5821 gttatgaccc cggcagaagc ctatcagaaa gtcgtacgct acaaaaccga gcatatccgg
     5881 ctggatgatt ttagcggcag aattgccgcc agcatgctcg tcccatatcc cccaggcatt
     5941 cctgtactga tgccgggcga acggatgccg caaggtaaca agggcattat tggctatctg
     6001 cgcgccctgc aggaattcga taagcaattc ccaggttttg aacatgagat ccagggcgtt
     6061 aacgtcgatg aaaacgggga cttctgggtc agagcgattg tggaagaaga acgcgatggg
     6121 cagtcgctac ccggccacat aacctttaaa cgccaggtct ctggtattaa aaagggccgc
     6181 cagtaggcgg gggaaagaac aataccgtcg gatcgcctgc aatccgacgg caatattata
     6241 gaatagctga gggaaacgtt atggcagagc aatcttcaga tgtaaaaaat agtgacaata
     6301 gcgccggagg acataagctg gggcttatat ccctcatcgc tattgtcgtt agctccatgc
     6361 tgggaggcgg cgttttcagt ctgccgcaaa acacggcggc ctcttctgca ctagggccag
     6421 taatcattgc atggattatt gccgggatcg gtatttattt tatcgcaaac tcctttcgcg
     6481 tactatcaga tctgcgcccg gaccttcagg ctggagtgta tatgtatgcc caggaaggtt
     6541 ttggttcctt tattggcttt aacgtcgcct ggggctactg gctgatgacc gcatttggca
     6601 acgttgcctt tgcggtaatt ttaatggatg cttttaatca gtttatgccg ggggttttta
     6661 cggatggtaa taatctgaac tccattatct gcggctcagt gctgatctgg ggctataatt
     6721 ttcttgttct gtccgggacg aaagtggccg gatttgttaa caccttggga accatcgcca
     6781 agctcgtccc tctgatccta ttcgtattac tgctgggtgt gctgatcgat tattcggacc
     6841 tgtttaaaaa cttttgggga gaatcacccg cgatcctcag tggcaacgga aatgacgccg
     6901 caccagtatc gctgctgtct cagattctgg caccgatgac cgtaacgctc tgggccttta
     6961 ttggcgtcga gggagcggtt gttctatccg gacgtgccaa aaataaacag gacgtaggga
     7021 aagccacact gatcggcttt ctgctggcgc taattattta tatgttaatg tccattctgc
     7081 cgttcggtgt aatgcctcag gcagaactag cgcaggtcag taacccctct accgcaggcg
     7141 tgctcgccca cgttgtcggt cagtggggcg actggctgat gagcgcgggg ctgattattt
     7201 ctgttctggc cggctggctt gcctggacca tgctatgcgc tgaaataccg atggtagcag
     7261 gacagaaagg cgctttcccg cgcgcgtttg cccgaacgaa cagtaaacag gcggccaatg
     7321 tgtcactgtg ggttagtagc tttgtgatgc aggcggccat gttacttgtt tatttttcaa
     7381 ataatgcttg gaccaccatg tataatatca gtgcgctgat ggttgttccg gcctatatta
     7441 ccaccacgct gtatctgacc agtatttgca gaaacggaaa atatgatcag tatgcaaaaa
     7501 aaggccgcag gctggctttg tccagcggtg ttttaggctc gctattctgt ctgtttattc
     7561 tttacgccag ccagattgag tatgttgcat taattcctat cctgttgacc tgtggtctgc
     7621 cggtctttat ctggtcaaga aaagaaaaaa atgatggtca gccgatattt accaaaaaag
     7681 agctgattta tctgattatg ctgttgctgt gcgatattat cgtcgttgcc ctgtttattg
     7741 gtggatattt aaaggtataa gtccgagtta aatctttact gggctagcaa tgccgccttt
     7801 taaacaagaa gggcggcaat cttactgaaa ttagtatgct gcaaatcggt aatgctgcca
     7861 acttactgat ttagtgtatg atggtgattt taaggtgctt acgtggcttc catttccatc
     7921 agatgtcctt cctgctccgc tactgaaggc
//
LOCUS       DACAPB010000193         7918 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.193, whole genome shotgun sequence.
ACCESSION   DACAPB010000193 DACAPB010000000
VERSION     DACAPB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.193"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..509
                     /locus_tag="IT904_003958"
     CDS             <1..509
                     /locus_tag="IT904_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAP3193666.1"
                     /translation="QAQYNFVGASEQLESAHRSVVQTVRSSFNNINASISSINAYKQA
                     VVSAQSSLDAMEAGYSVGTRTIVDVLDATTTLYNAKQELANARYNYLINQLNIKSALG
                     TLNEQDLLALNNALSKPVSTNPENVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTS
                     NGHNPFRN"
     gene            657..1328
                     /locus_tag="IT904_003959"
     CDS             657..1328
                     /locus_tag="IT904_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAP3193667.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGISS
                     RSMGG"
     gene            1334..2494
                     /locus_tag="IT904_003960"
     CDS             1334..2494
                     /locus_tag="IT904_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP3193668.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(2532..3347)
                     /gene="ygiD"
                     /locus_tag="IT904_003961"
     CDS             complement(2532..3347)
                     /gene="ygiD"
                     /locus_tag="IT904_003961"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417511.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAP3193669.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGTLSNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQIG"
     gene            3463..4236
                     /gene="zupT"
                     /locus_tag="IT904_003962"
     CDS             3463..4236
                     /gene="zupT"
                     /locus_tag="IT904_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAP3193670.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4294..4464)
                     /locus_tag="IT904_003963"
     CDS             complement(4294..4464)
                     /locus_tag="IT904_003963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193671.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(4726..5379)
                     /gene="ribB"
                     /locus_tag="IT904_003964"
     CDS             complement(4726..5379)
                     /gene="ribB"
                     /locus_tag="IT904_003964"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAP3193672.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5483..5631)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5753..6043
                     /locus_tag="IT904_003965"
     CDS             5753..6043
                     /locus_tag="IT904_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HAP3193673.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     gene            6327..6878
                     /locus_tag="IT904_003966"
     CDS             6327..6878
                     /locus_tag="IT904_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAP3193674.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            6938..>7918
                     /locus_tag="IT904_003967"
     CDS             6938..>7918
                     /locus_tag="IT904_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP3193675.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     NRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNQTAVCVTPEQLTL
                     LGFTDEFIEKTQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWNHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQATNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGISLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQL"
BASE COUNT         2167 a         1967 c         1819 g         1965 t
ORIGIN      
        1 aacaggcaca gtacaacttt gtcggtgcca gcgagcaact ggaaagtgcc catcgtagcg
       61 tcgtgcagac cgtgcgttcc tccttcaaca acattaatgc atctatcagt agcattaacg
      121 cctacaaaca agccgtagtt tccgctcaaa gctcattaga cgcgatggaa gcgggctact
      181 cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgttg tacaacgcca
      241 agcaagagct ggcgaatgcg cgttataact acctgattaa tcagctgaat attaagtcag
      301 ctctgggtac gttgaacgag caggatctgt tggcactgaa caatgcgctg agcaaaccgg
      361 tttccactaa tccggaaaac gttgcaccgc aaacgccgga acagaatgct attgctgatg
      421 gttatgcgcc tgatagcccg gcaccagtcg ttcagcaaac atccgcacgc actaccacca
      481 gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc cccgtctgaa
      541 cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt
      601 tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga
      661 aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga
      721 caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa aagagtgatg
      781 aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg
      841 ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat
      901 acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgccag caggcaccag
      961 cccaggctgg catggcacca gaaaaccagg cgcaggccca gcaatccagc gggagtttct
     1021 ggatgccgct gatggccggt tacatgatgg ggcgtctgat gggcggcggc gcgggatttg
     1081 cacagcagcc gctgttctcc tcgaaaaacc cagccagtcc ggcttacggt aaatataccg
     1141 acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc gtaccgaaga
     1201 cggcaatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt tttggtgaat
     1261 ctgttgccaa acaaagcact atgcagcgta gtgcaaccgg tatctcttct cgttcaatgg
     1321 gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc gtgagaaagc
     1381 ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt gtgaagatgc
     1441 ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg ccgaactgca
     1501 ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga tgaccaaatt
     1561 ccgcattcca aaacacacct ggagttttgt gcgccagtca tggctgacgc accagccatc
     1621 gctttattcg cgtcttgatc tggcgtggga tggcactggt gaacctaaac ttctggaaaa
     1681 taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga tctggctgga
     1741 agatcagctt aacgccggta acttgccgga gggcagcgac cagtttaaca gtctgcaaga
     1801 aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt tgctgcatct
     1861 cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc aggactgcgc
     1921 aacggaagct gaaattgcta ctgagttcct ctacatcgat gatatcgggt taggtgaaaa
     1981 aggtcagttc acggatttac aggatcaggt aatttccaac ctgttcaaac tgtatccgtg
     2041 ggaatttatg ttgcgtgaga tgttctcaac caagctggag gatgcaggcg tacgctggct
     2101 ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac tgtgggagat
     2161 gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc atccgcaaat
     2221 ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga
     2281 gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga tgattgttca
     2341 gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta gctggctggt
     2401 gaacgatcaa cccgccggaa ttggcattcg tgaagaccgt gcattgatca cccaggatat
     2461 gtctcggttt tatccacata tttttgttga ataagccacg ataccggatg gcactcgcca
     2521 tccggtaatt gttagcctat ctgcaccgac agcatactca ggctgcccat ttctataccc
     2581 tcaaccggaa tggtaattgg ctcctgccca tcccacgcac ctaacacata caacaacggc
     2641 aaataatgct ctggcgttgg gttcgataac gtgccacctt catggtcgag gtaattcacc
     2701 agaggatgtt gttccactgg cccttgccac gtcagattcg ctttcacata ctcattaaac
     2761 gacgtcgccc acggatacgg tgaactatca ccgtgccact tcactgtgcg caggttatgc
     2821 accacgttac cgctggcgac caacattatt ccttcatctc gcagcgctgc cagtttgcgc
     2881 cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg caccatcggg
     2941 atatcagcgt caggatacat cttaatcagc acgccccacg agccgtggtc aaagccccag
     3001 gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg ctgtgccagc
     3061 gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag
     3121 tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt aaaccagtga
     3181 gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg
     3241 cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa aaacaatgct
     3301 ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt gatatcctca
     3361 caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc atcagttatt
     3421 ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt acctctcatt
     3481 ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt tctcgggcaa
     3541 aaaccctcga accgcttact ggcgttttcg ctaggttttg cggcggggat catgttgctc
     3601 atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc gcctgtgttg
     3661 ggttatggaa tgtttatctt cggtctgctt ggctattttg gcctggaccg catgttgcca
     3721 catgctcatc cgcaggattt aatgcaaaaa tcggtgcagc cgttgccaaa atcgatcaag
     3781 cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga agggattgcc
     3841 acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact ggccgtcgcg
     3901 ttgcacaata tccctgaagg tctggcagtg gcaggcccgg tttatgcggc aacggggtct
     3961 aaacgcaccg cgattctgtg ggcggggatt tctggactag cagaaattct tggtggtgtg
     4021 ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcggc aatcatggcg
     4081 gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct cgccaaagag
     4141 attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt gatgggattc
     4201 agtttagtgc tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc
     4261 gcatggacaa cttctgccag cttcacgaaa gccttattct accttttcgg ctgtctcttc
     4321 tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa
     4381 cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaaccagag caatcaatac
     4441 aatccagtac caggcgataa acatcctttt acccttaata tttaacaata ctaaacattt
     4501 agcgtataaa tttcacatat cctttttcgg atatattcat atggtcgcat aataacggaa
     4561 caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa attttctcat
     4621 agaaagagaa gcggaattaa catcttgcat agcagggaaa aataaaaacc gaagcctgct
     4681 acaacttcgg tttcttgatt aaggcagtaa attaagcagc ggttttcagc tggctttacg
     4741 ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt
     4801 attggcaaac tcaatacact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc
     4861 acacagtaca ccagccggtt taaagcctgc cagcgtcatc agatcaatag ttgcttcagt
     4921 atgaccgcca cgcgtcagta caccacctgc ctgagcgcga agtgggaaaa cgtggccagg
     4981 acgattcaga tctgacggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg
     5041 gtcagcggca gaaacaccgg tagtcacacc ttcagctgct tcaatggtca cggtaaaacc
     5101 ggtgccatag gcgctggtgt tattttctac catcattggc agatcgagtt gtttacggcg
     5161 atcttcagta atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg
     5221 ctcaacagtc atggtttctg ccgggaagat catatcacct tcgttttcac ggtcttcatc
     5281 atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc
     5341 gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa
     5401 ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga
     5461 gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc
     5521 ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa
     5581 ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact
     5641 ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta
     5701 cccttatcgc actacaatgg cactcaacac ctatcagtac agggaaacta caatgattga
     5761 cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag
     5821 ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagcgc agctgacgcg
     5881 cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg
     5941 tgaaaaactg gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca gtactgagat
     6001 caaaaagcag ccagatccag agactctccc cccaacgctg taaacattcg tctttaatgc
     6061 actgtaaatt atgaattaat cctctaattt atagtgcttt ctattacctt atcgcgtaat
     6121 cccattcaga aaaccatcac aaaaatgaat gagtacaatt gaaagtataa ttgcaatgta
     6181 ttttttaagt tttacttaat cgatatttag ataaactaat tttataaaaa acacaaaata
     6241 aaatatcata tttcaatgat tagtagtcct taaataacag ttactaatca ttctcttttc
     6301 tacttatctt tatataaagg aatattatgt ccgcttttaa aaagtcactt cttgttgctg
     6361 gcgtagcaat gattctttcc aataacgtgt ttgctgatga aggtcacggt attgttaaat
     6421 ttaaaggtga agttatctct gctccatgtt ctattaaacc aggtgacgaa gatttaactg
     6481 taaatctggg tgaagtagca gataccgtac tgaaaagcga tcagaaatct ttggctgaac
     6541 ctttcactat tcacctgcag gactgcatgc tgagtcaggg tggtactact tattcaaaag
     6601 cgaaggttac tttcactaca gcaaatacca tgacgggtca gtctgatctt ctgaaaaata
     6661 ccaaagaaac tgaaatcggc ggcgccactg gcgttggcgt acgtattctg gacagccaga
     6721 gcggcgaagt aactttaggt actccggtag taattacgtt caacaatacc aacagctatc
     6781 aggaactgaa tttcaaagct cgtatggaat ccccaagcaa agatgccacc ccgggcaacg
     6841 tttacgctca ggctgactac aagattgctt acgagtaatc gcatttggcc gggttcgccc
     6901 ggccacatca ttaacggatt aatgataagt ggatcagatg tataaaaaat taaaattaac
     6961 cacaataagc gaattgatta aaaatattta ttgttcatta tccgttatca tcattggttg
     7021 tgcgtcagct tatgccgttg aattcaacaa agatttaatc gaagccgaaa atcgtgaaaa
     7081 cgttaacctt tcccaatttg aaactgatgg ccaattaccc gtcggcaaat attcactaag
     7141 cactctgatt aataataaga ggacgccaat ccaccttgac ctccaatggg tattaattga
     7201 taaccaaact gcagtttgcg tgacaccaga gcaattaaca ttattaggat ttactgatga
     7261 atttattgaa aaaactcagc aaaacctgat cgatggttgt taccctatcg aaaaagaaaa
     7321 acaaattaca acttatctcg ataaagggaa aatgcaatta tccatatctg cacctcaggc
     7381 atggttaaaa tataaagatg caaactggac gcctcctgaa ctttggaatc atggtattgc
     7441 tggggcattt cttgactaca atttatatgc ctctcattat gcaccacatc agggcgataa
     7501 ttcgcaaaat ataagttcct atgggcaggc tggggttaat cttggggcct ggcgcctgcg
     7561 tactgattac cagtacgatc agtcatttaa caatggcaaa agccaggcga ccaacctgga
     7621 ttttccgcgt atttatttgt ttcgcccaat cccagcaatg aatgcaaaac taactatagg
     7681 tcaatacgat actgaatcct ctattttcga ctctttccat ttttctggca tttcgttgaa
     7741 aagcgatgag aatatgttac cgccagacct acgtggttac gcaccgcaaa tcacgggtgt
     7801 cgcacaaacg aatgcaaagg tcactgtctc acagaacaac cgtattattt atcaagaaaa
     7861 tgttcctcca ggcccatttg ctattaccaa tttattcaat acattacagg ggcaactt
//
LOCUS       DACAPB010000194         7874 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.194, whole genome shotgun sequence.
ACCESSION   DACAPB010000194 DACAPB010000000
VERSION     DACAPB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.194"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..218)
                     /locus_tag="IT904_003968"
     CDS             complement(<1..218)
                     /locus_tag="IT904_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193676.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPF"
     gene            447..1055
                     /locus_tag="IT904_003969"
     CDS             447..1055
                     /locus_tag="IT904_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP3193677.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1153..2544
                     /gene="selA"
                     /locus_tag="IT904_003970"
     CDS             1153..2544
                     /gene="selA"
                     /locus_tag="IT904_003970"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAP3193678.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2541..4385
                     /gene="selB"
                     /locus_tag="IT904_003971"
     CDS             2541..4385
                     /gene="selB"
                     /locus_tag="IT904_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAP3193679.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4575..5736
                     /gene="yiaY"
                     /locus_tag="IT904_003972"
                     /pseudo
     CDS             4575..5736
                     /gene="yiaY"
                     /locus_tag="IT904_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
     gene            5901..7439
                     /locus_tag="IT904_003973"
     CDS             5901..7439
                     /locus_tag="IT904_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP3193680.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMDEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(7658..7801)
                     /locus_tag="IT904_003974"
     CDS             complement(7658..7801)
                     /locus_tag="IT904_003974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193681.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
BASE COUNT         1863 a         1970 c         2196 g         1845 t
ORIGIN      
        1 atgaacggca gcggggcggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcagcggat tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatgcg caccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg
      241 gttatttgtc tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata
      301 tccatcagat agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat
      361 ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat
      421 atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg
      481 tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac
      541 tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc
      601 tggtgaccga agagggcgaa tgttggtttg attcgccgat catcgctgaa tatattgaat
      661 taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca
      721 aaattgaggc actggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg
      781 cgcgtccagc ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc
      841 gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca
      901 atctggcgac tatcgccatt gcctgtgccg tcggatatct caatttccgc cgcgttgcgc
      961 caggttggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg
     1021 aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat
     1081 ctcacgttac aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca
     1141 gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat
     1201 cgcttattgc gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg
     1261 gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtgg cagccagacg
     1321 ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaagcg
     1381 cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttggg
     1441 cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg
     1501 accctcgagt atgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcag
     1561 ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg
     1621 gtgttattga tgttggcggc cactgccagc ggaaaagagg tggtggtatc tcgcggcgaa
     1681 ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc
     1741 ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat
     1801 gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc
     1861 aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg
     1921 actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca
     1981 atgccgcagg agttgattgc ggcgggcgtc agtctggtga gtttctccgg cgacaagttg
     2041 ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc ccgcctgcaa
     2101 agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc
     2161 acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg
     2221 cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc cccccttgcc
     2281 gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt
     2341 ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga
     2401 cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt gccggtgatt
     2461 ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg
     2521 tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa
     2581 caaccttatt gcaggcgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc
     2641 gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgcctg
     2701 gttttatcga cgttcccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg
     2761 gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggca cagacccgtg
     2821 agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag
     2881 ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggttctgc
     2941 gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa
     3001 tggatgccct gcgcgagcat ctgcttcagt tgccggaacg cgagcacgcc agccaacata
     3061 gtttccgcct cgcgattgac cgcgcattta ccgtaaaagg tgccgggctg gtcgtcaccg
     3121 gtacggcgtt aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata
     3181 aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc aacagaaacc gccaatgccg
     3241 ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg
     3301 actggctgct tgccgatgtg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa
     3361 cccatacacc gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtca
     3421 cgggacgcgt ttcactgctg gaagataacc ttgctgaact ggtcttcgac accccgttat
     3481 ggctggcaga taacgaccgc ctggtattgc gcgatatctc tgcccgcaac acgctggccg
     3541 gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc
     3601 aatggctggc gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg
     3661 aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga
     3721 tgcgcgaatt gctgcaacag cctggttata ttcaggctgg ttatagcttg ttgaatgcgc
     3781 cggttgccgc ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac
     3841 atcgcgatga acctggtcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag
     3901 atgaagcgct ggtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc
     3961 atcacggttg gctgcatctg ccagatcaca aagcgggctt cagcgaagag cagcaggcca
     4021 tctggcaaaa agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gacctggcaa
     4081 aagagacggg aaccgacgag caggcaatgc gcctgacgct gcgccaggcg gcgcagcaag
     4141 gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg
     4201 ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg
     4261 atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg
     4321 gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga cgcattatta tttccggaaa
     4381 aataaggaaa tgattaatta atttttaaaa taaattaata caaatttctt atgattttaa
     4441 aaaaagcaca ttgtttaatg aatacaatgt gctttttatt agattaattt tgataatcaa
     4501 taatactgat catattttcg aatgaaaatc ctcagtaagc tgcccgccct tttttacact
     4561 ttcaggagtg tgttatggca tcttcaactt tctttattcc ttctgtgaat gtcatcggcg
     4621 ctgattcatt gactgatgca atgaatatga tggcagatta tggatttacc cgtaccttaa
     4681 ttgtcactga caatatgtta acgaaattag gtatggcggg tgatgtgcaa aaagcactgg
     4741 aagaacgcaa tatttttagc gttatttatg atggcaccca acctaaccca acctaaccca
     4801 accacggaaa acgtcgccgc aggtttgaaa ttacttaaag aaaataattg cgatagcgtg
     4861 atttccttag gcggtggttc tccgcatgac tgtgcaaaag gtattgcgct ggtggcagcc
     4921 aatggtggtg atatccgtga ttatgaaggc gttgaccgct ctgcaaaacc gcagctgccg
     4981 atgatcgcca tcaataccac tgcgggtaca gcatcagaaa tgactcgttt ctgcatcatc
     5041 accgacgaag cgcgtcacat caaaatggcg attgttgata agcacgtgac tccgctgctt
     5101 tctgtcaatg actcctcgct gatgatcggt atgccgaagt cactgaccgc cgccactggt
     5161 atggacgcct taacgcacgc tatcgaagcg tatgtttcta ttgccgccac gccgatcact
     5221 gacgcttgtg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt agccgttgaa
     5281 gatggcagta atgcgaaagc gcgtgaagca atggcttatg cccagttcct cgccggtatg
     5341 gcgttcaata atgcttctct gggttatgtt catgcgatgg cgcaccagct gggcggtttc
     5401 tacaacctgc cacacggtgt atgtaacgcc gttttgctgc cgcatgttca ggtattcaac
     5461 agcaaagtcg ccgccgcacg tctgcgtgac tgtgccgctg caatgggcgt gaacgtgaca
     5521 ggtaaaaacg atgcggaagg tgctgaagcc tgcattaacg ccatccgtga actggcgaag
     5581 aaagtggata tcccggcagg cctacgcgac ctgaacgtga aagaagaaga tttcgcggtt
     5641 ctggcaacta atgccctgaa agatgcctgt ggttttacta acccgatcca ggcaactcac
     5701 gaagaaattg tggcgattta tcgcgcagcg atgtaatcat catttccaca acggctggca
     5761 aattgtcagc cgctttttca actatctctg taacccttgc ccgtaaattc gtgatagctg
     5821 tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct tgttatacct
     5881 cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt aagcccggcg
     5941 agtatggttt ccccctcaag ttaaaagccc gctatgacaa ctttattggc ggcgaatggg
     6001 tagcccctgc tgacggcgag tattaccaga atctgacgcc ggtgaccggg cagctgctgt
     6061 gtgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctggatgct gcgcacaaag
     6121 tgaaagataa atgggcgcac acctcggtgc aggatcgtgc ggcgattctg tttaagattg
     6181 ccgatcgaat ggaacaaaac ctcgagctgt tagcgacagc tgaaacctgg gataacggca
     6241 aacccattcg cgaaaccagt gctgctgatg taccgctggc gattgaccat ttccgctatt
     6301 tcgcctcgtg tatccgggca caggaaggcg gtatcagtga agttgatagc gaaaccgtgg
     6361 cctatcattt ccacgaaccg ttaggcgtgg tggggcagat tattccgtgg aacttcccgc
     6421 tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt gtggtgctga
     6481 aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaatcgtc ggtgatttac
     6541 tgccgccggg cgtggtgaac gtggtcaacg gcgcaggtgg ggaaattggc gaatatctgg
     6601 cgacctcgaa acgcatcgcc aaagtggcgt ttaccggctc aacggaagtg ggccaacaaa
     6661 ttatgcaata cgccacgcaa aacattattc cggtgacgct ggagctgggc ggcaaatcgc
     6721 caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat aaagcgctgg
     6781 aaggctttgc actgtttgcc tttaaccagg gcgaagtttg cacctgtccg agtcgtgctt
     6841 tagtgcagga atctatctac gaacgcttta tggaacgcgc catccgccgt gtcgaaagca
     6901 ttcgtagcgg taacccgctc gacagcgtga cgcaaatggg cgcgcaggtt tctcacgggc
     6961 aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgct gacgtgctca
     7021 caggcgggcg gcgcaagctg ctggaaggtg aactgaaaga cggctactac ctcgaaccga
     7081 cgattttgtt tggtcagaac aatatgcgcg tgttccagga ggagattttt ggcccggtgc
     7141 tggcggtgac cacctttaaa acgatggatg aggcgctgga gctggcgaac gacacgcaat
     7201 atggcctggg cgcaggtgtc tggagccgca acggtaatct ggcctataag atggggcgcg
     7261 gcatacaggc tgggcgcgtg tggaccaact gctatcacgc ttacccggca catgctgcgt
     7321 ttggtggcta caaacaatca ggtatcggtc gcgaaaccca caagatgatg ctggagcatt
     7381 accagcaaac caagtgcctg ctggtgagct actcggataa accgttgggg ctgttctgat
     7441 ataagaagct ggtcgcattg ggtattcatt gcctgatgcg acgcttacgc gtcttatcat
     7501 gcctacggga acctgaccgt aggccggata aggcgtacac gccgcatccg gcataaacaa
     7561 agcgcacgtt gttacaattt acgttttaaa cctacgtgcg caataaccca gactacgcca
     7621 acagaagtgc gtttctgggg cgtgttttgt caggagttta atctcgcgtt tcgatggtga
     7681 gcgggcgaac caggccgtaa atcaccattg gtgatccgag gccaatcagc aataaacggt
     7741 agataattga aggtttaata cgcttaatct gtactgtgga tgttttactg gagttattca
     7801 ttagtttggc caatagttcc actatttcaa tatgataaac ctcgtcctgt tgaaaaacca
     7861 gacagcacat ttca
//
LOCUS       DACAPB010000195         7768 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.196, whole genome shotgun sequence.
ACCESSION   DACAPB010000195 DACAPB010000000
VERSION     DACAPB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.196"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..460
                     /locus_tag="IT904_003975"
     CDS             <1..460
                     /locus_tag="IT904_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP3193682.1"
                     /translation="FTSLKKLRPQSVNSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWL
                     FYAYDRIRKTVVAHVSGERTLATLERLPGLLSAFEVVVWMTDGWPLYESRLNGELHVI
                     SKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(493..1143)
                     /locus_tag="IT904_003976"
     CDS             complement(493..1143)
                     /locus_tag="IT904_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3193683.1"
                     /translation="MREPAEPVLSEMIAQALRQKIIAGELLPGARLSERELTSQLDIS
                     RNTLREVFRLLTQEGLLRYQPNRGVCVAVPTDADIIDIYRIRRLIECDALRNAYPYHP
                     AITMMERAVNEARRALREENWGEVGTANMQFHAAIVALSDSERLMRLYQHISAELRLA
                     FGQLADKEMLHSPYVEKNAGIILLLKAGENGNAAATLETYLNISERTILAALARSR"
     gene            1483..2727
                     /locus_tag="IT904_003977"
     CDS             1483..2727
                     /locus_tag="IT904_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP3193684.1"
                     /translation="MAVDNINQHKSFVQNRRSSLLAAIFLMATSAIGPGFITQTATFT
                     ATLGVAFAFGILASILIDFVVQQNIWRVVTLTQMNAAEVANRAIPGSGYLLAVLVIFG
                     GLVFNIGNIAGAGLGLNALFGLSPKLGGLISAAIAIYIFASRRAGVALDRMLIALGVV
                     MIVLTLYVALVSHPPLGMALRETVFPSNINFAAITTIVGGTVGGYISYAGAHRLLDKG
                     ISGKEHIKEVSSAANRGIIVVGIMRYVLFLAIFGVVASGVTIDLSSHNANPASQAFLA
                     AAGNVGLRIFGIVLWAAALSSVVGAAWTSVSFISVFRPGITERQRNIATLIFIAFTLV
                     VYLCLGTAPAALLVFAGGFNGLVLPLGLTLFLWVGWRRADLMDGYRYPRWLLWLGMIT
                     CIMTWYMAIMSVSTIFNFLKIA"
     gene            2736..3509
                     /locus_tag="IT904_003978"
     CDS             2736..3509
                     /locus_tag="IT904_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HAP3193685.1"
                     /translation="MNTIDINSDLGESFGQWHMGDDARMLTVVSSANIACGFHAGDPA
                     GMRATLKAAAANQVCCGAHVGYPDIQGFGRREMNMADDDLTASVIYQIGALAGLARAA
                     GTRVSYVKPHGALYNTIAHDRRQGMAVINAIKDYNASLPLVVLAGSPLAQLAREQGLQ
                     VVAEAFADRAYHQDGTLVSRREAGAVLHDTQAIASRMVQLVREGGVVSVESVFTPISA
                     QSICVHGDSPGAVEMAIKVRAALEQEGIQIAPFCPGEQQ"
     gene            3506..4312
                     /locus_tag="IT904_003979"
     CDS             3506..4312
                     /locus_tag="IT904_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HAP3193686.1"
                     /translation="MNINYPATPQALEEAAQARLRIRKGYDKPTAGMAPGMTQVNMIA
                     LPRDWAFDFLLYAQRNPQSCPVLDVLEAGQCHTMLAAGGRVDTDIPRYRVWRDGELAK
                     EVTDAREIWQSHPDLVTFLIGCSFTFETPLMQAGIDVRHISEDCNVPMYRTNRPCREA
                     GRLKGELVVSMRPVPAHQVADAVTITARYPAVHGAPVHIGEPQALGIQDINKPDFGDP
                     VTLRPGEIPVFWACGVTPQAAVMASRVPFAVSHAPGHMFITDIPNAAWQQ"
     gene            4325..5908
                     /locus_tag="IT904_003980"
     CDS             4325..5908
                     /locus_tag="IT904_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HAP3193687.1"
                     /translation="MRFLPCSLSSFLVELGTLDDTLALFAALQAAPLSGVEEIIPAAR
                     TLLIHFDPTLTQCHQLSAAIRQLDGQMQAQRTGKVVTIPVQYTGDDLAEMAEYLGLSV
                     EALIQRHTGSLWQVAFCGFAPGFAYLVSREAGIQVPRRASPRTRIPAGSVGLAGEFSG
                     IYPHASPGGWQIIGRTTETLFALDRQPPALLQPGMQVQFVDVTRAPVCVPVVKPQPLQ
                     QSASGSAVMSVISPGLQTLFQDAGRARQSSMGISPSGALDQAAWRRANWLVGNPGHLP
                     ALEITAGGFRARICAPMVVALTGAPCPVMVTRADGAHFTASTEAPLALEAGDQLRLGA
                     PARGVRSYLARRGGWAVTPQLGSASRDTLAQVGPEPLQAGVKLYAGTTPPYSAVQPAV
                     IGLPSLPAPGETITLDIIPGPRCDWFNDEALDRLTSQCWQVTPQSNRIGLRLQGELPL
                     TRSDNRELPSEGTCIGAIQVPANGQPVLFLADHPLTGGYPVIGAVAHYHLDIAGQLPP
                     NCQIRFRAIHPFTVFEEYK"
     gene            5908..7653
                     /locus_tag="IT904_003981"
     CDS             5908..7653
                     /locus_tag="IT904_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP3193688.1"
                     /translation="MSAVATVPPHKVLVANRGEIAVRIIRACHEYGFASVAVYADPDV
                     DSLHCRLAGEAWALPGSSPAETYLNIEQLIDVARRSEATMVHPGYGFLSERAEFARAV
                     EAAGLIWIGPRPETIEQLGDKVQARKIAMAVGAPLVKGTPDPVSCAEDVIRFADEHGL
                     PVAIKAAFGGGGRGLKVAWFREEIATLYQSAVSEALTAFGRGECYVEQYLDRPRHIEA
                     QVIADEQGHVVVVGTRDCSLQRRNQKLVEEAPAPFLSAEIEAQIASAAQSICQRAGYR
                     SAGTVEFLLGANGVLSFLEVNTRLQVEHPITEETSGLDLVKEQLRVAHGLPLSITTPP
                     VPRGHAIELRINAEDPAKGFLPVPGTITRFDLPGGPGVRVDSGVTHGDTISGFYDSLM
                     AKLVIWAPDRDQALARARQAVKAFRIEGVASVLPFHEAVLHSPDFTKSFSVHTRWIET
                     DFSAELAEQVRPLPPGNSPLQRCWIEIDGRRHQLALPASFQAASGSAVPASLAETPQA
                     LQVAGAVTAPISGIVQRWLKTSGETVSEGETLAVMEAMKMEVPVLAARNGVLSIQIAA
                     GESCQQDGVLGKVTP"
BASE COUNT         1655 a         2138 c         2137 g         1838 t
ORIGIN      
        1 ttttacgtca cttaaaaaac tcaggccgca gtcggtaaac tcacgcatac aaccgggcag
       61 tgacgtcatt gtttgcgcgg aaatggacga acagtggggt tacgtcggcg ctaaatcacg
      121 ccagcgctgg ttgttttacg cgtatgacag gatacggaag acggttgtgg cgcacgtatc
      181 cggtgaacgc acgctggcca cgctggagcg tcttccgggc ctgctgtcgg cctttgaggt
      241 cgtggtatgg atgacggatg gctggccgct ttatgaatca cgcctgaatg gagaactgca
      301 cgttatcagc aagcgatata cgcagcgcat tgagcggcat aacctgaatc tgaggcaaca
      361 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
      421 agtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttaccgccag
      481 agctcaggta aattaccggc tgcgcgccag cgcagccaga atagtgcgtt cagaaatatt
      541 caggtaggtt tcaagggtgg cagcggcgtt accgttttca cccgctttca gcaacaaaat
      601 tatccccgcg tttttctcca catagggtga atgcagcatc tctttatctg ccagttgacc
      661 aaaggccagg cgtaattcgg ctgaaatatg ctggtaaagc cgcatcagac gctcactgtc
      721 tgaaagtgcg acaatggctg catggaattg catattcgcg gtgccaacct ccccccagtt
      781 ttcctcccgc aaagcgcgac gcgcctcgtt aacagcacgc tccatcatgg taatcgccgg
      841 gtggtatggg taagcattac gcaatgcatc acattcaatc aaccgccgga tgcggtaaat
      901 atctatgata tcggcatctg tgggtaccgc cacacacacg ccccggtttg gctggtaacg
      961 caacagccct tcctgggtaa gcaaacgaaa aacttcacgc aacgtgttac gggaaatatc
     1021 caactggctg gttaattccc tttccgagag gcgggcacca ggcagtaact ctccagcaat
     1081 aattttctga cgtaatgcct gagcaatcat ttcactcagt acaggctctg caggttctct
     1141 catctgcctc tcactaacgg gggacaagcg gcaatattcc cataatccag aagaagcagc
     1201 aacttatcct caccccttcc cggcattatt gctggcatag ccgcgaacaa cgcaccaggc
     1261 cggtgcatgc agtgcgtcat tgctgtgcac agttgttctt gcgtgttatc tcactctttt
     1321 aaaaccatgc attaacaaat attttacatt cgtatttttt ccactacgat caatattgtt
     1381 catcaattta tctataattg atgaacaatg aaataacttt aacccaacaa tggaatggtt
     1441 tttgcttaac aacatgacat ccccccgact ggagtgcatg caatggcagt tgataacatc
     1501 aatcagcata aaagctttgt tcaaaaccgg cgttcatctc ttctcgccgc catttttctg
     1561 atggcgactt ccgccattgg cccgggtttt attactcaaa ccgccacctt caccgccacc
     1621 ctgggtgtcg cattcgcgtt cggtattctg gcttctatcc tgattgactt tgtcgtacag
     1681 caaaatatct ggcgcgtggt aaccctgaca caaatgaatg cagcagaggt agcaaaccgg
     1741 gcaataccgg gcagtggcta cctgttggct gtgttggtta tctttggcgg actggtgttc
     1801 aacattggca acatcgcggg cgccggttta gggctgaatg ccttgtttgg cttgagccct
     1861 aaactgggtg ggcttatcag cgcagctatt gcgatttata tttttgcttc acgccgtgct
     1921 ggtgtagcgc ttgaccgcat gcttattgcg ctgggggttg tcatgatagt gctgacactc
     1981 tacgtggcac tggtttcaca cccaccattg ggcatggcac tacgtgaaac cgtgttcccc
     2041 agcaatatta actttgcggc aatcaccact attgttggcg gtactgtcgg gggttacatc
     2101 agttatgcgg gtgctcaccg cctgctcgat aagggcatca gcggcaaaga acacatcaaa
     2161 gaagtttctt cggcggccaa ccgcgggatt attgtggtcg gcatcatgcg ttatgtgctg
     2221 tttctggcta tttttggcgt ggttgccagt ggtgtcacca ttgatctctc cagccacaat
     2281 gcaaacccgg cttctcaggc attcctggcc gcagccggta atgtgggcct gcgtattttc
     2341 ggtattgtat tgtgggccgc agcactttcc agcgtggtag gcgccgcatg gacatccgtt
     2401 tcttttattt ccgttttccg cccggggatt actgagcgcc agcgcaatat cgccactctg
     2461 atatttattg cattcactct ggttgtgtat ctgtgcctgg gtacagcccc cgcagctctg
     2521 ctggtgtttg ctggcgggtt caatggcctg gtgcttcccc tggggttaac tttattcctt
     2581 tgggttggct ggcgccgggc agacctgatg gacggttacc gttacccacg ctggttgctc
     2641 tggctgggga tgataacctg catcatgacc tggtatatgg ccatcatgtc ggtttccact
     2701 atttttaatt tcctgaaaat tgcataaggc tgcctatgaa caccattgat attaacagcg
     2761 atcttggcga aagctttggt cagtggcata tgggtgatga cgcacgcatg ctgactgtgg
     2821 tgagtagcgc aaacattgcc tgtgggttcc atgccggtga cccggccggg atgcgggcca
     2881 cactcaaagc cgccgcagcc aaccaggtgt gttgtggcgc tcatgtgggt tacccggata
     2941 tacagggctt tggccgccgg gaaatgaata tggcagatga cgacctgact gctagcgtaa
     3001 tttaccaaat tggcgcactg gcgggcctcg cccgtgcggc gggcaccaga gtgagttatg
     3061 tcaaacccca tggagcgtta tacaacacaa ttgcacacga caggcgccag ggcatggcag
     3121 tcattaatgc gattaaagac tataacgcca gtttaccact agtggtgctt gccggttctc
     3181 ctcttgcaca gcttgctcgc gagcaggggt tgcaggttgt ggctgaagcc tttgccgacc
     3241 gggcctacca tcaggacggt acgctagttt cacgccggga agccggtgct gtgttgcacg
     3301 atacacaggc cattgccagc cgtatggtgc aactggtgcg cgaaggcggt gtagtgtcag
     3361 ttgaaagtgt gttcacgccc atctctgccc aaagtatctg cgtgcatggt gattcccctg
     3421 gtgccgtcga gatggcaatc aaagtgcggg ctgcactgga gcaggaaggt atccaaattg
     3481 cgcctttttg cccgggagaa cagcaatgaa cattaattac cctgcaacac cacaagcact
     3541 ggaagaggcg gcacaggctc gcctgcgtat tcgtaagggt tacgacaaac ccaccgccgg
     3601 gatggcgcca ggcatgaccc aagtgaatat gatagccctg ccacgcgact gggcatttga
     3661 ttttctgctc tacgcgcagc gcaacccaca aagttgcccg gtgcttgatg tcctcgaagc
     3721 cgggcaatgc cacactatgc tggcagctgg aggccgtgtg gatacggaca taccgcgtta
     3781 ccgggtatgg cgtgatggtg aactggctaa agaagtgacc gatgcccggg aaatctggca
     3841 aagccacccg gacctggtca cttttttgat tggctgcagc ttcacattcg aaacaccact
     3901 gatgcaggca ggcattgatg tccgccatat cagcgaagat tgcaatgtgc cgatgtaccg
     3961 aaccaatcgc ccctgccgtg aagctggcag gctgaaaggc gaactggtgg tctccatgcg
     4021 gccggttccg gctcatcagg ttgctgatgc cgtcactatt actgcccgtt acccggccgt
     4081 ccatggcgca ccggtacata ttggtgaacc gcaggcgctg ggtattcagg acatcaacaa
     4141 accagacttt ggcgacccgg tgacactccg ccctggagaa ataccagtgt tctgggcctg
     4201 tggcgttacc ccacaggcag cagtgatggc atcacgggtg ccatttgctg taagccatgc
     4261 ccctggccac atgtttatca ccgatattcc gaacgccgcc tggcaacaat aataaggatg
     4321 taatgtgcga tttttacctt gcagcctgtc cagtttttta gtggagctgg gcactcttga
     4381 tgacacgctg gctttgttcg ccgccttgca ggctgcgcca ttgtcaggag tggaagagat
     4441 cattcctgca gcccgtaccc tgcttatcca ttttgaccca actctcaccc agtgccacca
     4501 gttatctgct gccattcgcc agctcgacgg acagatgcaa gctcagcgca caggtaaagt
     4561 agtcaccatc cccgttcagt atactggtga tgatttggcc gagatggcgg aatacctcgg
     4621 gttgagtgtt gaggcgctca ttcagcgcca caccggctct ttgtggcagg tggccttttg
     4681 tggttttgcg ccaggtttcg cttaccttgt ttctcgtgaa gccggtatcc aggttccccg
     4741 ccgcgcaagc ccccgcaccc gtattccggc gggttctgtt gggcttgccg gagaattcag
     4801 cggtatttac ccccacgcaa gtcccggtgg ctggcaaata attggccgca ccacagaaac
     4861 actgtttgcc ctggacaggc agccgcccgc gttattgcaa cccggcatgc aagtgcagtt
     4921 tgtcgatgtc acgcgcgccc cggtctgtgt gccggtggta aaaccacagc ccctccaaca
     4981 atcagcttcc ggtagcgcag tgatgtcagt gatatcacca ggtttacaaa ccctgtttca
     5041 ggatgccggg cgcgcaaggc agtcaagcat ggggatatcc ccttcgggtg cacttgacca
     5101 ggccgcttgg cggcgcgcca actggctggt tggcaaccct ggtcacctgc ccgcactgga
     5161 aattactgcc ggtgggttca gagcccgtat ttgtgcccca atggtcgttg cgctaacagg
     5221 cgcgccctgc ccggtcatgg tcacccgtgc agacggcgca cattttaccg ccagcacaga
     5281 agcgccactg gcacttgaag caggcgacca gttacgactg ggagcgccag cacgcggcgt
     5341 gcgcagttac ctggcacgcc gtggcggctg ggccgttaca ccacaactgg gcagtgcctc
     5401 acgcgacacg ctggcacaag ttggcccgga accgttacag gctggggtta aattgtatgc
     5461 aggcaccacg ccgccataca gtgcggtaca gcctgctgtc atcggcctgc cttcattacc
     5521 ggcacccggc gagaccatca cgctggatat catccctggg ccacgctgcg actggttcaa
     5581 cgacgaagcg ctggaccggc tcaccagcca gtgctggcaa gtcacgccac aatctaaccg
     5641 cattggcctg cggttacagg gcgagttacc gcttacacgc agtgacaacc gcgaattacc
     5701 cagtgaagga acttgcattg gtgccatcca ggttccggcg aacgggcaac cggttctgtt
     5761 tctggccgac cacccgttaa ccggtggtta cccggtgatt ggtgctgtcg cccactacca
     5821 tcttgatatt gcgggccaac tcccgcccaa ctgccagatt cgctttcggg ccattcaccc
     5881 ttttactgtt tttgaggagt ataaataatg agcgctgttg ctaccgttcc cccacacaaa
     5941 gtgctcgtcg cgaaccgggg cgaaattgcg gttcgcatta tccgggcctg ccatgaatac
     6001 ggttttgcca gtgtcgcggt ctatgctgac ccggacgtcg acagcctgca ttgccgcctg
     6061 gctggtgaag cctgggcatt acccgggtcc agcccggcag agacttacct gaatatcgaa
     6121 caactgatcg acgttgcccg ccgcagtgaa gccacaatgg tgcacccagg ttacggcttt
     6181 ttgtctgagc gggctgaatt cgcccgtgcc gtggaagccg ccgggcttat ctggattggc
     6241 ccacgcccgg aaaccatcga gcaattgggt gataaagtcc aggcgcggaa aattgccatg
     6301 gcagttggtg caccgctggt taaaggtacg ccagacccgg tttcttgcgc ggaggacgtt
     6361 atccgctttg ccgatgaaca cggcctgccc gttgctatca aggctgcctt tggtggtggt
     6421 gggcgcgggt tgaaagtagc gtggtttcgg gaggaaatcg ccacacttta ccagtcagcc
     6481 gtcagtgaag cgctgacagc cttcgggcgc ggggagtgtt atgttgaaca gtatctcgac
     6541 cgcccgcgcc atattgaagc gcaggttatc gccgacgagc agggccacgt agtcgtggtg
     6601 ggtacccgcg actgctctct gcaacgccgc aaccaaaaac tggtagaaga agccccggcg
     6661 ccttttctca gtgctgaaat cgaagcacaa attgccagtg ccgcgcagtc catttgccag
     6721 cgtgccggtt accgcagcgc aggcacggta gagtttttgc tgggtgccaa tggtgtgttg
     6781 tcgtttcttg aagtcaatac ccgtttgcag gttgaacacc caataactga agaaaccagc
     6841 ggtctggatc tggtcaaaga acagctacgc gttgcccatg ggttaccact gagtattacc
     6901 acaccaccag tgccgcgtgg ccacgccatt gaactgcgga ttaatgcaga agacccggcg
     6961 aaaggttttc tcccggtgcc gggtacgatt acacgctttg atctgccagg aggaccaggg
     7021 gtacgcgtgg acagtggtgt cacccatgga gacactattt ctggcttcta tgattcactg
     7081 atggcgaaac tggtcatttg ggcaccagac cgcgaccagg cgctggcgcg tgcgcgccag
     7141 gcagtcaaag cctttcgcat cgaaggagtg gcatcagtgc tgcctttcca tgaagcggta
     7201 ctccattccc cggattttac taaaagcttt agtgttcata cgcgctggat tgagactgat
     7261 ttttctgctg agcttgctga acaggtacgc cctttacccc caggtaactc cccgctacaa
     7321 cgttgctgga ttgaaattga tgggcggcgc catcaactgg ctctcccggc cagtttccag
     7381 gctgcctccg gtagtgctgt tccggcaagt ctggctgaaa caccccaggc attacaggtt
     7441 gccggtgcag taactgcgcc catctccggc attgtgcagc gctggcttaa aacttccggc
     7501 gaaaccgtca gtgaaggcga gactctagca gtaatggaag caatgaagat ggaagtaccg
     7561 gtacttgctg cccgcaacgg agtgctgtca atccagattg ctgcaggaga aagctgccag
     7621 caggatggtg tgttgggtaa ggttacgccc tgacccaggc gttaacagtt tatggttaca
     7681 gccacgcatt tgcgcccttc acggcaaaag tgcgtggtag cacgcattac tggtacgttg
     7741 cctgtcgttg taatagaggt gtacgtct
//
LOCUS       DACAPB010000196         7634 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.197, whole genome shotgun sequence.
ACCESSION   DACAPB010000196 DACAPB010000000
VERSION     DACAPB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.197"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(135..464)
                     /locus_tag="IT904_003982"
     CDS             complement(135..464)
                     /locus_tag="IT904_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP3193689.1"
                     /translation="MFNIGFLWLALSIGSEITGTSMIKKTNGFRRLWPSVLVVCAYST
                     CYFALTRAMSTIPVGVAYSLWCGFGIVGVTIFSMILYKQKPDLPAIFSMLLIISGGII
                     MNVFSNM"
     gene            complement(480..881)
                     /locus_tag="IT904_003983"
     CDS             complement(480..881)
                     /locus_tag="IT904_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP3193690.1"
                     /translation="MSSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIV
                     VLYCICYYSLSRAVKDIPVGLAYATWSGTGILMVSTLGILFYGQHPDTAAIIGMVIIA
                     SGIIIMNLFSKMGSEEAEETPVTNLDKKIAN"
     gene            complement(914..1579)
                     /locus_tag="IT904_003984"
     CDS             complement(914..1579)
                     /locus_tag="IT904_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193691.1"
                     /translation="MTEQQIDTIVNLILQRLQPAVLVMVTSADGYRDLIHQRLARCGE
                     RLHLALDETISDSERWQQIGDVIPAKTWQHKLPSTPYKALLLPFLSYPLAVDIVNGTL
                     QSPVAQRVHDALLAGIPVLALRYYCDPHSELNALRGTVHSDYAAHLSATLTGLSECGI
                     TLCSMNEMLEKLATDVSSQSPVSHHRRYLTVTDIVNNPALAKSPDAVLTDAAVDFLKA
                     QKK"
     gene            complement(1591..2211)
                     /locus_tag="IT904_003985"
     CDS             complement(1591..2211)
                     /locus_tag="IT904_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HAP3193692.1"
                     /translation="MIDALLQEKITTRLIDTAPTIPVGVSNRHVHLSQQDVEALFGVG
                     YQLTPFKPLRQPGQFAAEECVTVVGPKGSLTHVRVLGPVRPTSQLEISRADCFTLGIK
                     APVRESGQLENAGDALLVGPKGHVELHSQVICAWRHIHMSPQDARLLNVSNGQKVSVR
                     SHGERQLTFDEVVVRVRNDFALELHIDTEEANAAGLKNGAQVTLIR"
     gene            complement(2208..2672)
                     /locus_tag="IT904_003986"
     CDS             complement(2208..2672)
                     /locus_tag="IT904_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAP3193693.1"
                     /translation="MNSLGVIETRGLVAAIQAVDAACKAAGVTCIGYRKVGSGLVTVC
                     FDGEISAVYTAIERGIAVASATDHQAKSLVIARPERCVVEALSNLKGHPPRVESGQKP
                     HAELPTVAEMAVCEETAEVSTDTETAMPVPEKEVQAPIVEVKSHGHKKGKKA"
     gene            complement(2684..3631)
                     /gene="cutD"
                     /locus_tag="IT904_003987"
     CDS             complement(2684..3631)
                     /gene="cutD"
                     /locus_tag="IT904_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020316596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline TMA-lyase-activating enzyme"
                     /protein_id="HAP3193694.1"
                     /translation="MSANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANP
                     EGLSSQFQVMFSQDKCINCGDCVNVCPAGIHYRAEVNGEMKHFVNRNKDCIGCRKCEE
                     ICTQNALDIMGKDVTVSELMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSEC
                     KKMMINTAVETQGTTPLANYQKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLE
                     WLVDSGVNVIVRMPLIRGYNDSWEAITGAIDYVQKLAKRGNIRRIDILPYHQLGRKKY
                     ERLEMPYPIVEDPSYSVEELDKLEAFFAQFDFDIRLVRH"
     gene            complement(3683..7069)
                     /gene="cutC"
                     /locus_tag="IT904_003988"
     CDS             complement(3683..7069)
                     /gene="cutC"
                     /locus_tag="IT904_003988"
                     /EC_number="4.3.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline trimethylamine-lyase"
                     /protein_id="HAP3193695.1"
                     /translation="MANYNLTPRVKVLAERLLAHPSTLCVEHAGILSGLDGDIAGIPA
                     AVKPARRFYELMRQLPLAVSPDELIVGNQTHRPHGAIFHDESTAHRPSVFQFLNLNSD
                     LDAPDYKLVIEKGVLAIKQQLEEKTRSLGSAVSRSGMDEVNACRAAIYACDALMQLAQ
                     NLATSAEKLAATETNAYRKAELSESAAILHHIPARPARSFKEACQAFYLFQLALQLDN
                     GSYAVNPEGADKALLAYYQHDIANGLLTEAQAYEIVECLWFKLAELSEVRAACAIDGY
                     PMFDALLHGASLENAVINPLSEMFLNAQRNLSALNLPIRLFHGAHKTVTTLCAACNET
                     PVLEGLTPRIQRLRNHYLTVRPSVSIYRALAFTEVVKANPGMPTILLRAKAFRHACET
                     APILIQDDELIVGHPCGKPRAGAFSPDIAWRWVRDELDTMSTRPQDPFEISEEDKKTI
                     REEIVPFWEGRSLDEICEAQYREAGVWSFSGETFVSDLSYHQVNGGGDTCPGYDVLLF
                     TKGMNGIKADAEAHLAELSMENPEDIDRIYYYKAAIDTCEGVINYAHRIAARARELAA
                     VEQNAQRRAELLTIAEVNQNVPANPPKTLQEALQSIWTVESLFEIEENQTGLSLGRVD
                     QYCYPMFEADIREGRLTHEGALELMQAFIIKCAELMWMSSELGAKYFAGYQPFINLTV
                     GGQKRSGGDACNDLTYLIMDAVRFVKVYQPSLACRIHNQSPQKYMEKIVDVVKAGMGF
                     PACHFDDSHIKMMLRKGFDFEDARDYCLMGCVEPQKSGRIYQWTSTGYTQWPIAIEFV
                     LNRGRMVLFDSYQGLDTGDLKDLRTFEDFDAAVKKQVAHIIRLSAIGTVISQRVHRDV
                     APKPLMSLLVEGCMEKGKDVSAGGAMVNHGPGLIFSGLATYVDSMAAIRKLVYEDKKY
                     TLEQIRDALLANFEGYEGLRRDCLNAPKYGNDDNYVDQYALDITEWTERECRKYKMLY
                     STLSHGTLSISNNTPIGELTNATPNGRLAWMPLSDGISPTQGADKHGPTAIIKSVSKM
                     NVETMNIGMVHNFKFLKGLLDTPEGRHGLITLLRTASILGNGQMQFSYVDNEVLKKAQ
                     QEPEKYRDLIVRVAGYSAYFVELCKEVQDEIISRTVIEKF"
     gene            complement(7096..>7634)
                     /locus_tag="IT904_003989"
     CDS             complement(7096..>7634)
                     /locus_tag="IT904_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP3193696.1"
                     /translation="VFSYLPGCWRNGQDLQAREKMHNASCMAGMAFTNASLGITHSLA
                     HALGGVFRVPHGRANALLMAHVVAWNADYHGQCDTLAARKYARLAHLLDLPASTPREG
                     VTSLLVAIQTLKDEMNMPRGINDTGVNAADFDQRLAEMVAQALRDSCTPTNPREPDAR
                     ALTELYRQAWSGNIAQCH"
BASE COUNT         1825 a         1992 c         1900 g         1917 t
ORIGIN      
        1 cagagcctag ctgggggctg tgcacaagaa aacggctccc tgttgtggaa gccgctatag
       61 tgccgcagtt taaggaccgg tcaactgctc cttaactgat ccgcattgtg cgtgaaagca
      121 gacttttggg gtcattacat gtttgaaaat acattcataa taatgccgcc actaataatc
      181 agcaacatgg agaatatggc aggtaaatcg ggcttttgtt tataaagaat catcgagaaa
      241 attgtcacgc caacaatccc aaagccgcac cagagtgaat aggcaacacc aaccggaata
      301 gtactcattg ctctggtcag agcgaaataa cacgttgaat aagcacatac caccagaaca
      361 gaaggccaca gtcgcctaaa accattggtt tttttgatca ttgacgtacc ggttatttca
      421 gaaccgatgg aaagtgccag ccataaaaat ccaatattaa acatgagcaa ttccttttgt
      481 cagttagcga tttttttatc gaggttggta actggagttt cttccgcctc ttcactgccc
      541 atttttgaga acagattcat aatgataata ccgctggcta tgatgaccat accaataatg
      601 gcggcggtat ccgggtgttg accgtaaaat aaaatcccaa gggttgaaac catcaaaatg
      661 ccagtaccgg accatgtggc gtaagccaga ccaacgggga tatcttttac tgcccgtgaa
      721 agcgagtagt aacaaatgca atacagtacg acgattaggg ccagcagcag cgtttttgtc
      781 atcccctcac tgttatcgaa cattttaagt gtggatgttg cagaggtttc agaaaggatg
      841 accagtaaca tccatagcca gcattttgtt ttcgaagaca tatctaaact ccaggtttgt
      901 tatcgaggta aggttatttt ttctgtgctt tcaaaaaatc gaccgcagca tcggtcagta
      961 cggcgtcggg tgattttgcc agtgccggat tattgacgat atcggtgaca gtcagataac
     1021 gccgatgatg acttacaggc gattgactgc tgacgtctgt tgccagtttt tccagcattt
     1081 cattcatgga acataacgta atgccacact cgctaaggcc ggtaagcgtg gcggaaagat
     1141 gcgccgcgta gtcgctgtga acagtaccgc gaagcgcgtt taattcgctg tgcggatcgc
     1201 agtaataacg cagtgccagt accggaatac ccgccagtag cgcatcgtga accctttgcg
     1261 ctaccgggct ttgcagcgta ccgttgacaa tatctacagc cagcgggtaa ctcaaaaacg
     1321 gcagcaataa cgctttatag ggcgttgaag gcaatttgtg ttgccatgtt ttagcgggta
     1381 tcacgtcacc aatttgctgc caccgttcgc tatcactgat tgtttcatcc agcgccagat
     1441 gcaaccgttc gccacaacga gccaaccgct gatgaatcag atcccgatag ccatccgcag
     1501 atgtcaccat gaccagtacc gcaggttgca gccgctggag gatcaggttg acgatggtgt
     1561 cgatctgttg ttcggtcatg atggcctccc ttagcgaatc agtgttacct gcgccccgtt
     1621 tttcagtccg gcagcattgg cttcttccgt atcgatatgc agttcaaggg caaaatcatt
     1681 gcgcacccgt accaccactt catcgaacgt taactggcgt tcaccgtgac tgcgaacact
     1741 caccttctgc ccattgctga cattcaacag acgagcatcc tggggagaca tatgaatatg
     1801 ccgccaggcg caaatgactt gtgaatgcaa ctcaacatga cctttcggcc ccaccaacag
     1861 tgcgtcaccg gcattttcca gttgcccgga ttcacgcact ggcgctttaa tgccgagagt
     1921 aaaacaatcg gcacgtgaga tttccagctg gcttgtcggg cgaaccggtc caagtacgcg
     1981 gacgtgagtc agagaacctt tcgggccgac aaccgttaca cactcttcag cggcgaattg
     2041 cccaggctgg cgcaacggct taaagggcgt aagttggtag ccaacaccaa aaagtgcttc
     2101 aacatcctgc tgcgacagat gcacatgacg attagaaaca ccgaccggaa tagtgggagc
     2161 agtatcgatc agtcgcgtgg tgattttttc ctgtagcagt gcgtcaatca tgctttcttc
     2221 cctttcttat gcccgtggct tttcacttcc acgatggggg cttgcacttc tttctcaggt
     2281 acaggcattg cagtttcagt atctgtagaa acttcagcgg tttcctcgca gacagccatt
     2341 tctgcaactg tcggcagctc tgcatgaggt ttctggcctg attcgacacg tggcggatga
     2401 cctttcaggt tgctgagagc ctcaaccacg cagcgttccg ggcgagcgat caccagtgat
     2461 ttggcctgat ggtcggttgc ggatgccaca gcaataccac gttcaatggc cgtgtaaact
     2521 gcgctgattt ccccatcaaa acagacggtt accagcccgg aacctacttt gcgataacca
     2581 atacaggtca ccccggctgc tttgcaggcg gcatctacgg cctggattgc ggctacaaga
     2641 ccacgtgttt caattacgcc taaactgttc atttacggct cctttaatga cgaactaagc
     2701 gaatatcaaa atcgaactgg gcgaaaaacg cctccagttt gtccagctct tccacgctgt
     2761 atgacgggtc ttcaacaatc gggtagggca tctccagtcg ttcatacttt ttgcgcccca
     2821 gctggtggta cggcagtata tcgattcggc gaatattgcc gcgtttggcc agtttctgga
     2881 cgtagtcgat agcgccggta atcgcttccc acgaatcgtt ataaccacga atcagcggca
     2941 tacgaacaat cacgttgacc cccgaatcca ccagccactc cagattgcga cgcacgcctt
     3001 cgttgccaat tccgaacagc gttttatggt gattgctatc gatctgtttg atatcgaaca
     3061 ggaaggtgtc ggtgaccgga gccagcttct gatagtttgc aagcggggtt gtaccctggg
     3121 tctcgacggc ggtgttgatc atcatctttt tgcattcact gaacagggca acggcaaaat
     3181 cagtttgcaa gctcatctca ccgcccccaa tagtgacacc accgccggag gagacgtaaa
     3241 agtcatagtc ctgcatgatg atctccatca attcactgac cgtgacatct ttacccatga
     3301 tatccagtgc attttgggtg caaatttctt cacacttgcg acaaccaatg cagtctttat
     3361 tacggttgac gaaatgcttc atttctccgt tgacttccgc gcggtagtgg atccccgcag
     3421 ggcatacatt gacgcagtcg ccgcagttga tgcatttatc ctgagagaac atcacctgaa
     3481 actggctgct taacccttcc gggttggcac accacgggca gcgaagattg cagcctttaa
     3541 aaaaaatcag tgtgcggata ccgtcaccgt cgtagatcga atatttctgg atattgaaaa
     3601 ttcgtccgct taattcttta tttgcgctca cgcttgcctc ctgaaaatgt cggacatcct
     3661 tgtccggttg cgatccgttt tatcagaact tctcaatcac cgtacggctg atgatttcgt
     3721 cctgaacttc cttgcacaat tccacgaagt aggcgctgta accggctaca cggacgatca
     3781 aatcacggta tttttccggt tcctgttgtg cttttttcag tacttcgtta tcgacgtagc
     3841 tgaactgcat ctgaccgttg ccgagaattg atgctgtacg cagtagagtt atcaggccgt
     3901 ggcgtccttc aggtgtatca agtaaccctt tgaggaattt gaagttatgc accatgccga
     3961 tattcatggt ttcaacgttc attttgctca cagacttgat aatcgccgtt gggccatgct
     4021 tatccgcccc ttgagtcggg ctgatcccgt cagaaagcgg catccacgcc agacgaccat
     4081 ttggtgtggc attggtcagc tcaccaatcg gcgtattgtt ggagatcgac aacgtaccgt
     4141 ggctgagggt ggagtagagc atcttgtact tgcggcattc acgttcagtc cactcggtga
     4201 tatccagagc gtactggtca acgtagttat cgtcgttgcc gtatttcggt gcgttcagac
     4261 agtcgcgacg caagccttca taaccttcaa agtttgccag cagagcgtcc cgtatctgtt
     4321 caagggtata tttcttatct tcgtaaacca gtttgcggat ggccgccatt gaatcgacgt
     4381 aggtagccag accagagaaa atcagcccag gtccgtggtt taccattgcg ccaccggcag
     4441 agacgtcttt gcctttttcc atgcagcctt caaccagtag cgacatcagc ggttttggtg
     4501 ccacgtcccg gtgcacacgc tggctgatga ccgtaccgat ggcggacaag cgtatgatat
     4561 gagcaacttg ttttttcacc gcggcatcaa aatcttcgaa tgtgcgcaga tctttcagat
     4621 cgcccgtgtc cagcccctga tagctatcaa acagcaccat acgaccacgg ttgaggacaa
     4681 actcgatggc aattggccat tgggtgtagc cggtggaagt ccactggtaa atacgacctg
     4741 atttttgtgg ttccacgcag cccatcaggc agtaatcacg ggcatcttca aagtcaaagc
     4801 ctttgcgcag catcattttg atatgggagt catcgaagtg acaggctggg aagcccatcc
     4861 ccgctttcac cacatcgaca attttttcca tatatttttg cggcgactgg ttatggatac
     4921 ggcaagccag tgatggctga tacactttca caaaacggac tgcatccata atcagatagg
     4981 tgagatcgtt gcaggcatcg ccaccgctac gtttttgacc accgacagtc aggttgataa
     5041 atggctgata acccgcaaaa tatttcgctc ccagttcgct ggacatccac atcagctcgg
     5101 cacatttgat gataaatgcc tgcatcagct ccagtgcacc ttcatgagtc aggcgacctt
     5161 cacggatatc tgcttcaaac atcgggtagc agtactggtc aacacgccca agtgacaggc
     5221 ctgtctggtt ctcttcaatc tcaaacagtg actctaccgt ccagatgctt tgcagcgctt
     5281 cctgcaaggt tttcggcgga ttcgccggaa cgttctggtt aacttctgcg atagtcagta
     5341 gctctgctcg acgctgggcg ttctgttcaa cggcagccag ttcacgggca cgggcggcaa
     5401 tacgatgcgc atagttgatc accccttcac aggtgtcgat cgctgctttg tagtagtaaa
     5461 tgcgatcgat atcttccggg ttttccatgc tcagttcggc aagatgcgcc tcggcatcgg
     5521 ctttgatgcc attcatccct ttggtaaaca gcagcacatc gtagcccgga caggtatcac
     5581 ccccaccgtt aacctgatga taggagaggt cgctgacgaa tgtttcgcca ctgaacgacc
     5641 aaacgccagc ttcgcggtat tgtgcttcac aaatttcatc cagcgagcgc ccctcccaga
     5701 acggtacaat ctcttcgcga atggtttttt tatcttcttc gctgatttca aacggatcct
     5761 gcgggcgagt actcatggta tcgagttcgt cacgcaccca gcgccaggca atatcaggtg
     5821 aaaatgcgcc agcacgcggt ttaccgcaag gatgaccgac gatcagctca tcgtcctgaa
     5881 tcaaaatcgg cgcagtttca caggcgtgac ggaacgcttt agcacgcagc aggatggttg
     5941 gcattcctgg attcgctttc actacttcgg taaaagccag tgcacggtaa atagaaacac
     6001 tcgggcggac agtcaggtag tggttacgca gacgttggat acgtggtgtc aggccttcca
     6061 gcactggcgt ttcgttacag gcggcacata gtgtggtaac ggttttgtgg gcgccatgga
     6121 acagacgtat gggcagattt agcgcgctca ggttacgttg tgcattgagg aacatctctg
     6181 aaagagggtt aatcactgcg ttctcaaggc ttgcaccgtg caacagagca tcgaacatcg
     6241 ggtagccatc gatagcacaa gctgcgcgga cttcactcag ttctgccagt ttaaaccaca
     6301 aacattcgac aatttcgtaa gcctgcgcct cggtcagcag accgttggca atatcatgct
     6361 gataatacgc cagcagcgct ttatctgcgc cttccgggtt aacagcatag ctaccattat
     6421 ctaactggag tgctaactgg aacagataaa atgcctggca agcctcttta aaactacgag
     6481 caggacgcgc tgggatatgg tgcaaaatgg cggcactttc agataactca gctttgcgat
     6541 acgcatttgt ctcagttgca gccagttttt cggcgctggt ggccagattt tgtgctaact
     6601 gcatcagggc atcacaggca taaatcgcgg cgcggcaagc gttgacttca tccataccac
     6661 tgcggctaac ggcgctgccc agtgagcggg ttttctcttc caattgctgt ttgattgcca
     6721 gcacgccttt ttcgatcacc agtttataat ccggggcatc aaggtcgcta tttagattaa
     6781 gaaactgaaa aacagacggt cggtgggccg tactttcgtc gtggaaaatc gcaccatgcg
     6841 gacgatgagt ctgattgcca acaatcagtt catcggggct aacagcgagc ggtagctggc
     6901 gcatcagttc atagaaacgg cgagcgggtt ttactgcggc gggaatgccc gcgatatcac
     6961 catccagccc actcaggatc ccggcatgtt caacgcacag ggtactgggg tgagccagta
     7021 aacgttcagc cagcactttt acgcgcggtg ttaaattgta gtttgccata aggtaagggc
     7081 ctccagttct tgttatcagt ggcattgagc gatattgccg ctccaggcct ggcgataaag
     7141 ttcagtcagg gcgcgggcat ccggttcgcg cgggttggtt ggggtgcaac tgtcacgcag
     7201 tgcctgggca accatctccg ccaggcgctg atcgaaatca gcggcattca cgccggtgtc
     7261 gttaatcccc ctgggcatat tcatttcatc tttcagcgtt tggattgcga ccagcaggct
     7321 tgttactcct tcacgaggcg tgctggcagg cagatccaac agatgtgcca gacgtgcata
     7381 tttccgcgca gcaagggtgt cgcattgacc gtggtagtca gcattccatg ccacaacgtg
     7441 tgccattagc agggcatttg cgcgcccatg agggacacgg aaaacaccac caagggcatg
     7501 agccaggcta tgggtgatgc caagagaggc attggtaaac gccattcctg ccatacatga
     7561 ggcgttgtgc attttctccc gagcctgcaa atcctggccg ttacgccagc agcccggcag
     7621 gtagctgaac acct
//
LOCUS       DACAPB010000197         7535 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.198, whole genome shotgun sequence.
ACCESSION   DACAPB010000197 DACAPB010000000
VERSION     DACAPB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.198"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            441..1010
                     /locus_tag="IT904_003990"
     CDS             441..1010
                     /locus_tag="IT904_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP3193697.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1270..1662
                     /locus_tag="IT904_003991"
     CDS             1270..1662
                     /locus_tag="IT904_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193698.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSLPRTAQQLS"
     gene            complement(1672..2640)
                     /locus_tag="IT904_003992"
                     /pseudo
     CDS             complement(1672..2640)
                     /locus_tag="IT904_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2826..3236
                     /locus_tag="IT904_003993"
     CDS             2826..3236
                     /locus_tag="IT904_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193699.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKNNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3520..5140
                     /locus_tag="IT904_003994"
                     /pseudo
     CDS             3520..5140
                     /locus_tag="IT904_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            5184..5512
                     /locus_tag="IT904_003995"
                     /pseudo
     CDS             5184..5512
                     /locus_tag="IT904_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420459.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltranspeptidase"
     gene            complement(5552..6586)
                     /locus_tag="IT904_003996"
     CDS             complement(5552..6586)
                     /locus_tag="IT904_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HAP3193700.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(6589..7521)
                     /locus_tag="IT904_003997"
     CDS             complement(6589..7521)
                     /locus_tag="IT904_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP3193701.1"
                     /translation="MLATILAGMLSPMQSAVNGQLGHWLQDGNACAVISFASGLVVMF
                     FIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASASALGVATFQTA
                     LISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQWHSTSFILLAI
                     LPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAIRVALGHVTVQL
                     PDVWWMYLGGPLGLMSISLMAILVRGLGLLMLGVASTAGQLLGSVLIDVLIPSLGNTV
                     YLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
BASE COUNT         1921 a         1887 c         1929 g         1798 t
ORIGIN      
        1 tggttgtctg gagattcagg gggccagtct aaaccgttat ctcaccagct acacagtata
       61 cacacactga cgctgacagt agtcagcagg accacaataa tcagcgccag cagtatggta
      121 acgatacggt ctgtattccg gtagtagccg aaggggtcga cagaaccaca atacgggcaa
      181 cacactgtat tatgagtaat cgcgtttcca cagtccctgc acttcttttt gcgtatcacc
      241 atcactccct cctcacagcc ttacccgtaa ccggtttttt acattaaaaa acgacgctga
      301 aaattcaacc gtcagtccgg agacgaccgt tcgggttatc acagagtgcc tgagacagtg
      361 tcctgccgga ggtcacttca gggactctgc gtatttttta cgacgtggct attccgtatg
      421 aaccatacgg agatttaacc atgacctaca aatacaaccc cttctggcag caacgtattc
      481 gtgagacggt gcggcacgca ctgaatgttc atccccgcct gacggcattg cgggttgacc
      541 tgcgtctccc ggatgtaccg gcagcaacgg acgcagctgt gatatcccgc ttcatcaatg
      601 ccctgaaagc ccgaatcgac gcttaccaga aacgtaagca tcgggaaggt aaacgcgtgc
      661 atcccacaac cctgcattac gtctgggccc gggagtttgg ggagtgcaaa ggtaaaaaac
      721 actatcacct gatgctgctg gtcaaccggg atacctggtg tcgtgccggt gattaccgcg
      781 ctccggagtc tctggccggg atgattaaac aggcgtggtg cagtgccctg ggagtggatg
      841 tcgggtgcca tgccacgctg gtgcattttc cggcctggcc ggcggtgtgg ctggcgcgta
      901 atgatgacac cggctttcag caagtgctgg aacgtgctga ctatctggcg aaggagcata
      961 ccaaagctca ctgcaccggt gagcgcaatt ttggctgtag ccggagttga gcacgactgg
     1021 cgcgccaata tccggcaaac caaaagtatg ggttgttgtc tggcggcaac gatgtatcca
     1081 ctcgcccact gactgttttt tacgtcagtg ttccattgtg tgacaaccac agtgctgtaa
     1141 cagcaaccag cctgctttac ccaaactcca gtccagattt taactgaacc gccattcaca
     1201 ccctgaccac gctgcccgta ccgggacggc tgttgcctgc gtgtcgtctg gcggctgagg
     1261 tatatgacta tgtacgcaaa atcctttatc gctcttgatg gcaacggacg tctgacgggc
     1321 gcccgtactg cacaggccgc accttatgct aactacacct gccacttgtg tggcagtgca
     1381 ctcagatacc atccgcaata cgaaactgaa cttccctggt ttgaacacac tgacgacagg
     1441 ctgacagagc acggtcaaca gtgcccttat gtcaggccgg agcgcagaga aatacagttg
     1501 attaaacgtc tgcagcaatt cgtaccggat gccttacccg tggtgcgtaa agccagctgg
     1561 cactgcagac aatgtcacca cgattattat ggggagcagt actgcacaca ctgccagacc
     1621 ggaggtttca gtcttccccg gacagctcag caactgtctt gaacgtaaaa attatggagc
     1681 aacgtgatgg tacgattcat tccattcttc gttctttctg agcattgcgt tcaggatagt
     1741 cagcagcttg cgtatacagg caaccagcgc cacttttttg ggttttcctg cggcaagcag
     1801 tcgcatgtaa aacaccttta ttaccgggtt aaagcgggtt gcgacaagcg ctgccatgta
     1861 cagggctgtt cgcacaccag ccctgccgcc aaagatggtt cgtcgccctc tcatcgtgcc
     1921 cgagtcccgg tttactggaa ccataccgac aagcgcactg atttcacgcc tggagaggtt
     1981 accctgttcg ggtacttccg ccagcagcgc agcagccgtc atggtgccaa cacctttaat
     2041 actgctcagt cgcttagcaa gctctttgaa gtggctatgg atatggctgt tcatgtcagc
     2101 atctagtcgc gcaagctcgt cttccagtgc tttgatgatg atattaatgc ttttcctgtt
     2161 ctatggatga gcagggtgca accggtttct ctcggcaacc agcatcgtaa tcaactgccg
     2221 cctacgaact accattgcag tgagaacctg acattcagta tccggcagtg ctctgataaa
     2281 gcgttcccgc tccggatgcc ggttgagaac ctcagccatc tgggaaagca ctctggcatc
     2341 aatactatca gttttcgcga ggtatcccat ggcgcgggca aagtcacgag cttgtctggg
     2401 gttaaccacg gcaagctcaa aaccttctgc ctgaagcgca cagactatgg cggtttcaag
     2461 tcctccagtg gctttcatta aaaccagcgc aatggcgtac attctcaact cagcaatgat
     2521 ggcctcaaag ccatcactgt cattgccgac agaaaattgt tcaatagcgc tgctggcggc
     2581 aatatccagt atcgctttcg aaacatcgat acccacacaa agtgaaccta gctgactcat
     2641 gattacccat ccttgcaaat gcgttatggc gtactgacaa ctgttcgggt ttctgatgag
     2701 tggctcagtg tatgcgccat tcgctgagcc acgggctatg aaccccttga gggatcgggc
     2761 tgcatacacc ttgccaaatc taatcacagt tattttaacc aattgtcaag atacaaggag
     2821 gagatatgcg aattctgaac tgctatatgg cgaatgacag caaaggccat tttgttacgg
     2881 cgaaagaagc cgcgaagaac aaccgacagg acgttttgtg ctgtgtgtcc tgtggttgtc
     2941 cgttaacact taagcggggc aatgacggac aaccgccgtg gtttgaacat gaccagatga
     3001 ctgttgctgc gaaaatcctg ctgcgatgca cctggcttga cccggcagag aaagaggccc
     3061 gtcgtttgca tctgcagggc atgacggttc cggattatac ggtgaaggtg agaaagtggt
     3121 tttgtgtgat gtgtgacgaa gattatgagg gggaaaagtg ctgcccgcgt tgtggtactg
     3181 gggtatacag cagggagggg gggctgcagg aaggcaactg gaaggatagg aactgagcgg
     3241 ttcagtcgcg gaaatcagtt gcagagctgt aatgatatct ccgggagtgc tgcgttggta
     3301 ggggcatccc gggatagaga aattttagct ctttgccctg aatgattgta gacgccccga
     3361 gtactcgtac cggtctgagt tattacagtt agatattcac tttttctccc gcatacttca
     3421 catctgtcaa cgacggatga aaagtggttc ttccgcaacc tgggtggaag aaaatggatg
     3481 aaactttctg gcgaacaagg atctaagctg ttttaagtta tgggcaaccc aatgcactct
     3541 cttaagacac ttctacagtt accttgcgga tggcgatgca gtcgacaaat tattagctct
     3601 gacggtataa ccctccatct ccacggaaaa cgcaaaacag cacaatgtcc tgaatgcttt
     3661 aagcgtagcg actctgttca tagttgttgt cggcgccgga tacagcatct accctgatca
     3721 gggcagacgc tatggcttgt attttccgtc cgccactggt actgccgtaa cccagcttgt
     3781 tcacgtaaaa tttttgccga gtcgcttgct cccttcgccg gttcacacca acagtcttca
     3841 caggcgttac aaaatttaca acgtcaactg ggattaatag ctggaggtga ggctggaaaa
     3901 cgggctgcaa cggcagcggg tctccgttgc agtgctgata ctcttcttcg cagggttatc
     3961 aataccccgg agacgaaaca gtcaggcgcg cctcatgtcg gtattgatga gtgggcgtgg
     4021 catcggggcc actgctgcgg tatgttaata gtcaatcttg atactcaccg tcccctcgtc
     4081 ctgcttcccg gccgtgatca gcgtacgctg gcgacctggt tcagaaaata tccggaaata
     4141 caggttgtct cgcgtgatcg cagtggagtc tatgcgacag tagcacgtga aggtgcacct
     4201 caggccagac aggtggccga tcgatggcac ctgctaaaaa atattggtga tgagcctgaa
     4261 cgaatgatgt acagacatat gcctctgata cgtcttgttg tcagagagtt atcactgaag
     4321 aaatcacctg agccagaaat atctgtgcct gtagcatcgc tccgtcgtcc ggaacgcctt
     4381 aaacagcaaa cccgcaaaaa acggcatcag cattggacag aggttatggc cctgcataac
     4441 aagggatgta gtttcaggga aatatcccgt attacaggcc tgtcgcgagt gacagtcagt
     4501 cgctgggtgc gttcaggaac attccctgaa atgtcaaccc ggcctccaaa gcgagggctt
     4561 ctggacccat ggagggagtg gttaaaagag caacgagaaa gtggtaatta taatgccagc
     4621 cggatatggc gggaaatggt ggcccagggg ggggacaggc agtgaaacca tcgtcaggga
     4681 tactgttgcc aaatggcgta aaggctggaa cccaccggtt actactgccg caagacttcc
     4741 ttcagtgtcc cgggtaagcc gctggttgat gccctggaga ataatcaggg gcgaagaaaa
     4801 ttatgcatcc cgatttatta gtctgatgtg tgaaaaagaa ccggagttga aaatagcaca
     4861 gcaactggta ctcgagttct accgtattct gaaaacctaa aataaatcac agctaagcag
     4921 ctggttcact cgagtccacg aaagcggctc agcagaactt cggtgcgtag ctgcggggat
     4981 ggaagctgat gctgcggcta tatgtgaggc aatcagcagt cgctggagta atggtgttgt
     5041 cgaaggtcat gtaaatcgcc tgaaggtgct gatacgccag atgtatggtc gagccggatt
     5101 tgaactactt aggcggaggg taatgagtcc actggcatga aaatcttcac ctaggttgcg
     5161 gaagagccac ttttgcactg ttcgtgacag tatggccttt atgtactcac tttgttaccg
     5221 gtagctgaat catcactacc gtgctgcaaa tggtggtgaa cagcatcaat tgtggaatga
     5281 atgttgccga agcgaccaat gcgcctcgtt tccaccatca gtggttgccg gacgagctgc
     5341 gtgttgaaaa gggtttagtc cggatacgct caagctgctg gaagcaaaag gtcagaaagt
     5401 ggcgctgaat gaggcgatgg gcagcacgca aagcattatg gttgggtcgg atggtgagtt
     5461 gtacggcgca tccgactcgc gcttggtgga tgatttaacg gcggggtatt aattgacatc
     5521 ccccggcgca gtgtgccggg ggataatggt tttatgcggt caataatcgt ccgggattat
     5581 cgatacagag ttttttgagg atcgttttat cgacgccgcg ctccgccaga taggccagaa
     5641 aaacatctgg aacaaatccc cagccattac cgccattttt agcccacatt tgtttaagga
     5701 agacatcgtg gctgagaaca agctgatcgg cgtatcccaa ctcaatgaga tgagcgacgg
     5761 catcggcagt ctcctgcacc cctggcgcta ttccctcttt cgggaaggta atgtctaggc
     5821 caatcatgtc gaattccagc cagacaccct tatcaagcat tttccgctga tacgccacgt
     5881 cttttcctga cggatccgag tgtgcgagag agactttatt tggcgagacg cccatttcac
     5941 ctaacacaat gtcgagtact tcatcaccgc gacgaagcca gcccggcatg tgaatattca
     6001 tcgccacatg aggattgtta atctgtgcca gcgaggcagc ccgcaagctg ttatgctcgg
     6061 cttcggtaaa tgtcggtgag acacctattt caccgatcat tccggcacga atatccgtat
     6121 cgccaatccc ttgattcaat tctttatcaa tcgttgccgc cagttcatca accgttttat
     6181 gaattctctg gctttcaaat ttttccaggt agggaccgga ggaagcaaca atattcagac
     6241 ctgtttttaa tgcgacttct cgtaatgcct gcgcatctcg tccgatagac tcagaccctg
     6301 tggcatcaac aatggtgcga ccaccgagcg agataaagtt attaatttcg aagatcacat
     6361 cttcaatagg tttgcggtcc atattatcag cgcagcaata aggatcgtgt tttaatgccc
     6421 actggatttc tgccgtgact tttttatcaa caagccgttg tgaaaaagga tagcaaggag
     6481 catccacaac agaggaaaga tcgttgaaca gatgttcatg aggaagtgtt agccccatat
     6541 cttcacgggc gacggggccc gtcacagttt gtagataatc tttcatgatt atacctccat
     6601 tttcttcatt gttttcgagg ttctgtattc cggaatagtg gtaacaattg caccgaccag
     6661 tgcaaacagt gttccgatga tcgtgaccag ataaacggta ttgcccagcg atggaattaa
     6721 tacgtcaatc aatactgatc ccagtaactg gcccgccgtt gaagcaaccc ccagcatcag
     6781 taaccccaga cctctgacca aaatggccat taaactgata gacattagtc caagcgggcc
     6841 gccaagatac atccaccaga catctggtaa ttgaacagtg acgtgcccta atgcgacacg
     6901 aatagctagc gctgtgccta aaacacaaaa tccaacgatg aaattccagg tgattgaaac
     6961 cagcatggat cccgtagctt cagctacttt tgcatttcca gcgggctgcc atcctgcaag
     7021 caaaccagct aagaacggaa gaatggcaag taagatgaaa gaggtagaat gccactgcgg
     7081 tgacacaaca aaaatcgtag caataaccgc aaataatgca ccgatgatgc gataaggggt
     7141 aaagtatttt ttctcgtcga caccgatacc aaagcgatcg caaagtaaac cagacagcaa
     7201 tagcgcggag atcagtgctg tttgaaacgt tgcgaccccg agtgcgctgg ctgatgcgcc
     7261 ttcagaaaat accaccatcg ctccacagag accggcaaac cagttccaga gaggaacttt
     7321 tcggttttta atcagagaag gaattgccgc aaattgttgc cgggtttctt ttcgcgccat
     7381 aataataaaa aacatcacga ctaaaccact ggcaaaggag ataactgcgc atgcattacc
     7441 atcctgtaac cagtgaccta attgaccatt caccgctgat tgcatagggg agagcatacc
     7501 cgcaagaatc gtggcaagca tccataaggg agttg
//
LOCUS       DACAPB010000198         7524 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.199, whole genome shotgun sequence.
ACCESSION   DACAPB010000198 DACAPB010000000
VERSION     DACAPB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.199"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..235)
                     /locus_tag="IT904_003998"
     CDS             complement(<1..235)
                     /locus_tag="IT904_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193702.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKK"
     gene            555..2585
                     /gene="malS"
                     /locus_tag="IT904_003999"
     CDS             555..2585
                     /gene="malS"
                     /locus_tag="IT904_003999"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP3193703.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRNGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            2763..4016
                     /gene="avtA"
                     /locus_tag="IT904_004000"
     CDS             2763..4016
                     /gene="avtA"
                     /locus_tag="IT904_004000"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAP3193704.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARAVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4167..4640)
                     /locus_tag="IT904_004001"
     CDS             complement(4167..4640)
                     /locus_tag="IT904_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP3193705.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(4742..5590)
                     /gene="yiaJ"
                     /locus_tag="IT904_004002"
     CDS             complement(4742..5590)
                     /gene="yiaJ"
                     /locus_tag="IT904_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAP3193706.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            5791..6789
                     /gene="yiaK"
                     /locus_tag="IT904_004003"
     CDS             5791..6789
                     /gene="yiaK"
                     /locus_tag="IT904_004003"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAP3193707.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDTQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            6801..7268
                     /locus_tag="IT904_004004"
     CDS             6801..7268
                     /locus_tag="IT904_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP3193708.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         1914 a         1911 c         1858 g         1841 t
ORIGIN      
        1 cctttttcct tggtgttccg gaagggtatt ttcgcaaatc aggaagtgaa ctactctttg
       61 cactattgcg agaatactct tgtttactgc ttacctgttt attactagct ttagttaagt
      121 gggacttttg actcgctgtt gttgtgtgcg tcttcgctaa cacctcactc gaaaacacca
      181 gagtgagtaa cataagaatc atcgccccat atcgtcgtat gggagtcaaa atcatcaggt
      241 ctcctggtcg gatttaatca ttccaacacc ttatattttt cacaaatttg agagttgaat
      301 ctcaaatcat atcaaaaata gctgtcaaga gcaccccaag gaatagtcca aatctgaaac
      361 tatgtcacgt gttaacgatt cagattggcg ctaaatcgca gaaaatgtgg gggttatcgc
      421 aaaattcagc cgttttttgc gcgagatcgc tcacccttgc ttctcatcct gtggacttac
      481 cgctcaggga tgagttttgt ttggcttatc gctggcaaac tgtctgaaat cgcagcaata
      541 aggactcatc cgccatgaaa ctcgccgcct gttttctgac actccttcct ggcttcgccg
      601 ttgccgccag ctggacttct ccggggtttc ccgcctttag cgaacagggg acaggaacat
      661 ttgtcagcca cgcgcagttg cccaaaggta cgcgtccact aacgctaaat tttgaccaac
      721 agtgctggca gcctgcggat gcgataaaac tcaatcagat gctttccctg caaccttgta
      781 gcaacacgcc gcctcaatgg cgattgttca ggaacggcga atatacgctg caaatagaca
      841 cccgctccgg tacgccaaca ttgatgattt ccatccagaa cgccgccgaa ccggtagcaa
      901 gcctggtccg tgaatgcccg aaatgggatg gattaccgct cacagtggat gtcagcgcca
      961 ctttcccgga aggagccgcc gtacgggatt attacagcca gcaaattgcg atagtgaaga
     1021 acggtcaaat aatgttacaa cccgctgcca ccagcaacgg tttactcctg ctggaacggg
     1081 cagaaactga cacatccgcc cctttcgact ggcataacgc cacggtttac tttgtgctga
     1141 cagatcgttt cgaaaacggc gatcccagta atgaccagag ttacggacgt cataaagacg
     1201 gtatggcgga aattggcact tttcacggcg gcgatttacg cggcctgacc aacaaactgg
     1261 attacctcca gcagttgggc gttaatgctt tatggataag cgccccattt gagcaaattc
     1321 acggctgggt cggcggcggt acaaaaggcg atttcccgca ttatgcctac cacggttatt
     1381 acacacagga ctggacgaat cttgatgcca atatgggcaa cgaagccgat ctacggacgc
     1441 tggttgatag cgcacatcag cgcggtattc gtattctctt tgatgtcgtg atgaaccaca
     1501 ccggctatgc cacgctggcg gatatgcagg agtatcagtt tggcgcgtta tatctttctg
     1561 gtgacgaagt gaaaaaatcg ctgggtgaac gctggagcga ctggaaacct gccgccgggc
     1621 aaacctggca tagctttaac gattacatta atttcagcga caaaacaggc tgggataaat
     1681 ggtggggaaa aaactggatc agaacggata tcggcgatta cgacaatcct ggattcgacg
     1741 atctcactat gtcgctagcc tttttgccgg atatcaaaac cgaatcaact accgcttctg
     1801 gtctgccggt gttctataaa aacaaaatgg atacccacgc caaagccatt gacggctata
     1861 cgccgcgcga ttacttaacc cactggttaa gtcagtgggt ccgcgactat gggattgatg
     1921 gttttcgggt cgataccgcc aaacatgttg agttgcccgc ctggcagcaa ctgaaaaccg
     1981 aagccagcgc cgcgcttcgc gaatggaaaa aagctaaccc cgacaaagca ttagatgaca
     2041 aacctttctg gatgaccggt gaagcctggg gccacggcgt gatgcaaagt gactactatc
     2101 gccacggctt cgatgcgatg atcaatttcg attatcagga gcaggcggcg aaagcagtcg
     2161 actgtctggc gcagatggat acgacctggc agcaaatggc ggagaaattg cagggtttca
     2221 acgtgttgag ctacctctcg tcgcatgata cccgcctgtt ccgtgaaggg ggcgacaaag
     2281 cagcagagtt attactatta gcgccaggcg cggtacaaat cttttatggt gatgaatcct
     2341 cgcgtccgtt cggtcctaca ggttctgatc cgctgcaagg tacacgttcg gatatgaact
     2401 ggcaggatgt tagcggtaaa tctgccgcca gcgtcgcgca ctggcagaaa atcagccagt
     2461 tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac gacacttttg ctgaagcagg
     2521 gctacggctt tgttcgtgag catggcgacg ataaagtgct ggtcgtctgg gcagggcaac
     2581 agtaactttt ccggcttccc gttcgtcagt acctcgggaa gccgccaacc aggataaaat
     2641 gtcagcccta atcagcgttg caggataaag caccgctcac tcttcaacag accgatttgc
     2701 accccagcaa atgtagcgtt attgttacct tccttgctac agagttcgac agatatcccg
     2761 ctatgacatt ctcccttttt ggtgacaaat ttacccgcca ctccggcatt acgctgttga
     2821 tggaagatct gaacgacggt ttacgcacgc ctggcgcgat tatgctcggc ggcggtaatc
     2881 cggcgcagat cccggaaatg caggactact tccagacgct actgaccgac atgctggaaa
     2941 gtggcaaagc gactgatgca ctgtgtaact acgacggtcc acaggggaaa acggagctac
     3001 tcacactgct tgccggaatg ctgcgcgaga agttgggttg ggatatcgaa ccacagaata
     3061 ttgcactaac aaacggcagc cagagcgcgt ttttctactt atttaacctg tttgccggac
     3121 gccgtgccga tggtcgggtc aaaaaagtgc tgttcccgct tgcaccggaa tacattggct
     3181 atgctgacgc cggactggaa gaagatctgt ttgtctctgc gcgtccgaat attgaactgc
     3241 tgccggaagg ccagtttaaa taccacgtcg attttgagca tctgcatatt ggcgaagaaa
     3301 ccgggatgat ttgcgtctcc cggccgacga atccaacagg caatgtgatt actgacgaag
     3361 agttgctgaa gcttgacgcg ctggcgaatc aacacggcat tccgctggtg attgataacg
     3421 cttatggcgt cccgttcccg ggtatcatct tcagtgaagc gcgcccgcta tggaatccga
     3481 atatcgtgct gtgcatgagt ctttccaagc tgggtctacc tggctcccgc tgcggcatta
     3541 tcatcgccaa tgaaaaaatc atcaccgcca tcaccaatat gaacggcatt atcagcctgg
     3601 cacctggcgg tattggtccg gcgatgatgt gtgaaatgat taagcgtaac gatctgctgc
     3661 gcctgtctga aacagtcatc aaaccgtttt actaccagcg tgttcaggaa actatcgcca
     3721 tcattcgccg ctatttaccg gaaaatcgct gcctgattca taaaccggaa ggagccattt
     3781 tcctctggct atggtttaag gatttgccca ttacgaccga gcagctctat cagcgcctga
     3841 aagcacgcgc cgtgctgatg gtgccggggc acaacttctt cccagggctg gataaaccgt
     3901 ggccgcatac gcatcaatgt atgcgcatga actacgtacc agagccggag aaaattgagg
     3961 cgggggtgaa gattctggcg gaagagatag aaagagcctg ggctgaaagt cactaaaaat
     4021 tgcctgatgc gctacgctta tcaggcctac atgatttctg caatatattg aatttggaag
     4081 aatttgtagg ccggataggg cgtttacgcc gcatctggca taaacaaagc acgctattcc
     4141 ggcgttgaga aacgccggaa aacgtttcaa acaggctgct gccgtagccg atgccgctgc
     4201 actttctcga catccatgca ctgcaacgcc tttgtcgggc aggcttcaac acaggccggt
     4261 cccgtctccc gatgccagca cagatcgcac ttaatcgccc tcgccttttt gcgcgaagaa
     4321 acgacctcca tcgcaccaaa cgggcaagcc agcatacagc ttttacagcc aatgcaacgt
     4381 gtttgttcaa cgaaaatatg cccttgttcg cggcttatcg cgtcaacagg gcagacattc
     4441 gcgcacggtg catcttcaca ctgatgacag gctactgccg tggtccagct gtggtcttta
     4501 atgacacgaa tacgggaaat aaactcgtct ggtgacaacg cagcgcaatc ctgattctcc
     4561 tgatgcgaca ctgcgcaagc cacttcacag gtacggcaac cgatacattt cgtcgcatcc
     4621 gcaataataa accggttcat gatcactcct ttgcactaaa acagcagagt gccagagagc
     4681 gcgtccctac tgctttgatc caacgaagga aatgttaaag ttttggcact tgtccaaagc
     4741 gttatgtgat cgcgcccagg tcatccctga cagtaaatcc cagttcatta gaaatagcct
     4801 gcgcggtttc acgcagtggt ttcaggagat ttttctctcc cacctgtttc agacgtgatg
     4861 tcgaaagcga aatcgacacg gcgtacggca cccgcccatg aatatcaaac accggaacag
     4921 caatacagga gacgccgagt tcgttttctt ctctgtccat cgccgctccg ctttcacgaa
     4981 tgtgcgccag ttcgtcgaac atcgcgggca gctcggtaat ggtattgcgg gttaacggct
     5041 ggatctcatg ctgatggctt tcccagtatg acttcacgta gtccgggtga ccaaacgcca
     5101 tgtagatctt gcccattgcg gaacagtaga gcggcatatg ctggccaata taggcacggg
     5161 ttcgcagcat cccggttgtg ggttccagct tataaatcaa aatagcgtga tcgtcttcgc
     5221 ggctggagaa gttaatggtt tcaccagtgg cgatgttcag tgcctcaaga tgcggagcgg
     5281 cgatatgaat gatattcagc gaagacagcg ccttctgccc gacggcaata aatttggtgg
     5341 tcaggcgata actccctgcg gcaggcgcgg tggtcacata gccgcaggac tgtaatccct
     5401 gcaataagcg atggacggta ctcttattta aaccagccag ctccgaaaga tgcgccaacg
     5461 gacaaccgtt tggatagttg ctcaaaatct caatcagcat caacccgcga aacagactct
     5521 ggcttccggc tggacgctct ttttcctgcg ccatctcgtt ctcttttttt cccatcactt
     5581 ctttccccat tttgtcgcgt cctgatggta gcgcaaagtg taccgtagtt cacgatctcg
     5641 acagataatt tataaccaat tgatttttat gtcttttgaa attcatcaat cagattgctt
     5701 tgttaaaaag tgatcgatat atttgaaatc aagtttcgca tattgaaatt ttaagccaaa
     5761 aaagcgatca aaaaaacaag gaagcctggg atgaaagtga catttgagca gttaaaagca
     5821 gcctttaatc gggtcttaat ttcacgcggc gttgacagcg aaacggctga cgcctgtgca
     5881 gagatgttcg cccgcaccac cgaatccggc gtttattctc acggcgttaa tcgtttccct
     5941 cgtttcattc aacaactgga aaacggcgat atcattcctg atacccaacc caaacgtata
     6001 accagcctcg gcgcaattga acagtgggac gcccagcgtt cgatcggtaa cctgacagcg
     6061 aaaaagatga tggatcgcgc cattgaactg gctgccgatc acggtattgg tctggtggca
     6121 ctacgtaatg ccaaccactg gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa
     6181 ggctatattg gcatttgctg gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa
     6241 gagtgtcgca tcggcactaa cccgctgatc gtcgccattc cttccacgcc gatcaccatg
     6301 gtcgatatgt cgatgtcgat gttctcttac ggcatgttag aagttaaccg tctggcaggt
     6361 cgtcagctcc cggtcgatgg tggctttgat gatgagggca atttgaccaa agaacctggc
     6421 gttatcgaga agaatcgccg cattttgccg atgggctact ggaaaggttc tggcatgtcg
     6481 attgtgctgg atatgatcgc tactctcctt tccgacggcg catccgttgc cgaagtcacc
     6541 caggacaaca gcgacgaata cggcatttca caaattttta ttgccattga agtggacaag
     6601 cttatcgacg gtcccacccg cgatgccaag ctgcaacgca tcatggatta cgttactagt
     6661 gccgagcgtg ctgacgaaaa tcaggccatt cgcttacccg gccatgaatt tactaccctg
     6721 ctggccgaaa accgccgtaa cggtattacc gttgatgaca gcgtgtgggc caaaatccag
     6781 gcgttataag gagataagtc ataatttttg gacatatcgc gcagcccaat ccgtgccgtt
     6841 tgcccgccgc cattgaaaag gcgctcgatt ttctgcgcgc taccgatttc aacgccctgg
     6901 agccgggcgt tgtcgaaatc gacggcaaaa atatttatgc gcaaattatc gatttaacca
     6961 cacgcgaagc ggtggaaaac cgtccggaag tgcatcgtcg gtatatcgat attcagtttc
     7021 tggcgtgggg tgaagagaaa atcggcattg ctattgatac gggaaataat aaagtcagcg
     7081 aatcactatt agagcagcgc gatattattt tttatcatga cagtgaacat gaatcattta
     7141 tagaaatgat accgggcagc tacgcaattt tctttccgca ggatgttcac cgaccaggtt
     7201 gtattttgca aacagcctct gagatacgaa aaattgtggt taaagtcgca ttaacggcgc
     7261 tgaattaatc gaataatcgt ctacgccaga acgcctgggt aatgttattg ctctaactat
     7321 attaataatt caggttgctg cacatgcaac atgaattatg acagacatag cctcaaggaa
     7381 tagctatgaa aaaaatactc gaagcaatac tggcgattaa tctcgccgta ctttcctgta
     7441 ttgtgtttat aaacattatt ttaagatatg gttttcaaac aagtatttta tctgtagatg
     7501 aattgtcgcg ttatttattt gtct
//
LOCUS       DACAPB010000199         7512 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.200, whole genome shotgun sequence.
ACCESSION   DACAPB010000199 DACAPB010000000
VERSION     DACAPB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.200"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..179
                     /locus_tag="IT904_004005"
     CDS             <1..179
                     /locus_tag="IT904_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP3193709.1"
                     /translation="IDAAMTNISVIQLKLQAGRKLTQEETTRLNVVLDYIEAVTATDT
                     STAPDVIWPELQEA"
     gene            complement(151..246)
                     /locus_tag="IT904_004006"
     CDS             complement(151..246)
                     /locus_tag="IT904_004006"
                     /inference="COORDINATES: protein motif:HMM:NF014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP3193710.1"
                     /translation="MWLDYLYAPERSDTSGTSDIKWPTPPAVQAR"
     gene            510..1700
                     /locus_tag="IT904_004007"
     CDS             510..1700
                     /locus_tag="IT904_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAP3193711.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRARI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            1713..2231
                     /locus_tag="IT904_004008"
     CDS             1713..2231
                     /locus_tag="IT904_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP3193712.1"
                     /translation="MAMPRKLKLMNVLLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            2288..2563
                     /locus_tag="IT904_004009"
     CDS             2288..2563
                     /locus_tag="IT904_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP3193713.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            2596..2715
                     /locus_tag="IT904_004010"
     CDS             2596..2715
                     /locus_tag="IT904_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP3193714.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            2708..5155
                     /locus_tag="IT904_004011"
     CDS             2708..5155
                     /locus_tag="IT904_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP3193715.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAVVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSTEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAVFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIVGGALALTGVIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAIMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDALDQTAARTQAATYGSG
                     SYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            5170..5649
                     /locus_tag="IT904_004012"
     CDS             5170..5649
                     /locus_tag="IT904_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193716.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            5649..6812
                     /locus_tag="IT904_004013"
     CDS             5649..6812
                     /locus_tag="IT904_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP3193717.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEVIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKDKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            6894..7112
                     /gene="ogrK"
                     /locus_tag="IT904_004014"
     CDS             6894..7112
                     /gene="ogrK"
                     /locus_tag="IT904_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HAP3193718.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(7432..>7512)
                     /locus_tag="IT904_004015"
     CDS             complement(7432..>7512)
                     /locus_tag="IT904_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="stress-induced glycyl radical protein that can
                     replace an oxidatively damaged pyruvate formate-lyase
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAP3193719.1"
                     /translation="GYAVRFNSLTKEQQQDVITRTFTQSM"
BASE COUNT         1861 a         1928 c         2161 g         1562 t
ORIGIN      
        1 tgatagacgc agcaatgacc aatatcagcg tgattcagtt aaagctgcag gccgggcgca
       61 aactgacgca agaagaaact acccgactta acgttgtgct ggattatatc gaggctgtga
      121 cggcaacaga taccagcacc gcaccggatg tcatctggcc tgaactgcag gaggcgtagg
      181 ccatttaata tctgacgtac cggaagtatc ggaccgctcc ggtgcgtaca ggtaatccag
      241 ccacaaatta tattgcgcca gtacctcaga cctgccgggc tattgcttac tgttcatgta
      301 tttattggcc tgattaatca gtagctgtct ttctggtcgt gtggatcctg caaatcctga
      361 atggtcggaa ataccgcaat aaatacaggc gggctgattg cccgcctttt ttatctgttg
      421 tttcatcccc tgaccagcca ggacaaatag catctcatgc cctgcacaac tgaaaatacc
      481 actcacccat taaccacgga gttaaacgga tgagtgacta tcatcacggc gtgcaggtgc
      541 tggagattaa cgacggcaca cgcgtcattt ccaccgtatc cactgccatt gtcggcatgg
      601 tctgtacggc cagcgatgcg gatgcggaaa ccttccccct caataaaccg gtgctgatta
      661 ccaatgtgca gagtgcaatt gcaaaggccg gtaaaaaagg cacgctggcg gcatcgttgc
      721 aggccatcgc tgaccagtca aaaccggtca ccgttgttgt gcgcgtggaa gacggcaccg
      781 gcgacgacga agaaacgaaa ctcgcgcaga ccgtttccaa tatcatcggc accaccgacg
      841 aaaacggtca gtacaccgga ctgaaagccc tgctggcggc ggagtcggta accggtgtta
      901 aaccgcgtat tcttggtgtg ccggggctgg ataccaaaga ggtggctgtt gcactggcat
      961 cagtctgtca gaagctgcgt gctttcggat atatcagcgc atggggctgt aagaccattt
     1021 ccgaggtgaa agcctaccgc cagaatttca gccagcgtga actgatggtt atctggccgg
     1081 atttcctcgc atgggatacg gtcaccagta ccaccgccac cgcgtatgcc accgcccgtg
     1141 cgctgggtct gcgtgccaga atcgaccagg agcagggctg gcataaaacg ctgtccaacg
     1201 tcggggtgaa cggtgttacc ggcatcagcg catccgtctt ctgggatttg caggagtccg
     1261 gcaccgatgc tgacctgctt aacgagtcag gcgtcactac gctgattcgc cgcgacggtt
     1321 tccgcttctg gggtaaccgt acctgctccg atgacccgct gttcctcttt gaaaactaca
     1381 cccgcaccgc gcaggtgctg gccgacacga tggctgaggc gcacatgtgg gcggtggaca
     1441 agcctatcac cgcaacgctg attcgcgaca tcgttgacgg catcaatgcc aaattccgtg
     1501 agctgaaaac aaacggctat atcgtggatg cgacctgctg gttcagcgaa gaatccaacg
     1561 atgcggaaac cctcaaggcc ggaaaactgt atatcgacta cgactatacc ccggtgcctc
     1621 ctctcgaaaa cctgaccctg cgccagcgta tcaccgataa atatctggca aatctggtca
     1681 cctcggttaa cagcaattaa ggagcctgac cgatggcaat gccgcgcaaa ctcaagttaa
     1741 tgaacgtcct tctgaacggc tacagctatc agggcgttgc aaagtccgtc acgctgccaa
     1801 aactgacccg taagctcgaa aactatcgcg gtgcggggat gaacggcagc gcaccggtag
     1861 acctcggcct tgatgacgat gcgctgtcaa tggagtggtc gctcggtggc ttcccggatt
     1921 cggttatctg ggagctttac gccgcaaccg gtgtggatgc tgtgccgatt cgttttgcag
     1981 gctcttacca gcgtgacgat accggcgaaa cggtggccgt cgaagtggtc atgcgtggac
     2041 gtcagaaaga aatcgacacc ggcgagggta aacagggaga agacactgag tcgaaaatct
     2101 ccgtggtctg tacctatttc cggctgacga tggacggtaa ggagctggtc gaaatcgaca
     2161 ccatcaacat gattgagaag gtgaacggcg tcgaccggct ggagcaacac cgccgcaata
     2221 tcggcctgta attgtcatcc ggtcagcctg gctgaccggt taaccccgat tcagaagtga
     2281 gaaaaccatg aacaaagaaa atgtcattac cctggacaat ccggtcaaac gtggtgagca
     2341 ggttatcgaa caggtcacgc tgatgaaacc caatgccggg acgctgcgcg gtgtcagtct
     2401 ggctgcggtt gcaaactccg aagtcgatgc actgattaaa gtgctgccgc gcatgacggc
     2461 accgatgctg accgagcagg aggtcgccgc gctggaactg cctgaccttg tggcgctggc
     2521 cggtaaggtg gtcggttttt tgtcgccgaa ctcggtgcag tgacgttccc gaaaaatctg
     2581 tcggtcgatg acctgatggc ggatgtggca gtgatatttc actggccgcc atcagaactg
     2641 tatcccatga gcctgaccga actcatcaca tggcgcgaaa aggcgctccg gcgaagcgga
     2701 aacacgaatg agtaacaatg taaaattaca ggtattgctc agggctgttg accaggcatc
     2761 ccgcccgttt aaatccatcc gcacagcgag caaatcgctg tcgggggata tccgggaaac
     2821 acaaaaatca ctgcgcgagc tgaacggtca ggcatcccgt attgagggat ttcgcaagac
     2881 cagtgcacag ctcgccgtga ctggtcatgc acttgaaaag gcacggcagg aggccgaagc
     2941 ccttgccaca cagtttaaaa acaccgaacg tccgacacgt gctcaggcga aagtgctgga
     3001 atccgcgaag cgtgcggcgg aggacttaca ggcgaaatat aaccgcctga cggattccgt
     3061 taaacgccag cagcgggaac tggccgttgt gggaattaat acccgcaatc ttgcacatga
     3121 tgagcaggga ctgaaaaacc gtatcagtga aaccaccgca caacttaacc gtcagcgcga
     3181 cgcgctggcg cgtgtcagtg cgcaacaggc aaaacttaac gcagtcaaac agcgttatca
     3241 ggcaggcaag gaactggccg gaaatatggc ctcagtgggc gctgccggtg tggggattgc
     3301 ggcggcggga acgatggccg gagttaagct gctgatgccc ggttatgagt ttgcgcagaa
     3361 aaactcagaa ttacaggctg tgctcggagt ggcaaaagac tccaccgaaa tggccgcact
     3421 acgcaagcag gcgcgccagc tcggcgacaa taccgccgcc tcggcggatg atgcggccgg
     3481 tgcacagatt atcattgcga aagccggtgg ggatgttgat gccattcagg cggcaacgcc
     3541 ggtcacgctg aacatggcgc tggcgaaccg ccgcacgatg gaagaaaacg ccgccctgct
     3601 gatggggatg aaatccgcct ttcagctttc aaacgataag gtcgctcata tcggggatgt
     3661 tctctccatg acgatgaaca aaaccgccgc cgattttgac ggtatgagcg atgcgctgac
     3721 ctatgccgca cctgtggcaa aaaatgccgg tgtcagcatt gaagaaaccg ccgcaatggt
     3781 cggggcgctg catgatgcaa aaattaccgg ttcaatggcg gggacgggaa gccgtgccgt
     3841 gttaagtcgc ctgcaggcac cgacgggaaa agcatgggat gcactcaaag agcttggagt
     3901 gaaaacctca gatagcaagg gaaacacccg accagtattt acaattctga aagaaatgca
     3961 ggccagtttt gagaaaaacc ggctcggtac tgcccagcag gctgaataca tgaaaaccat
     4021 tttcggggag gaggccagct cagccgccgc cgtgctgatg actgccgcct caaccggaaa
     4081 gctggacaaa ctgaccgctg tgtttaaagc ctcagacggg aagaccgcgg agctggtaaa
     4141 tatcatgcag gacaacctcg gcggtgactt taaggagttt cagtccgctt atgaggcggt
     4201 ggggactgac ctgtttgacc agcaggaagg cgcgctgcgt aagctcacgc agacggccac
     4261 aaagtatgtg ttaaaactcg acggctggat acagaaaaac aaatcactgg cgtcaaccat
     4321 cggcatcatt gtcggtggtg cactggcgct gactggtgtc atcggtgcca ttggcctcgt
     4381 agcctggccg gttatcaccg gcatcaatgc catcatcgcg gcagcaggcg caatgggtgc
     4441 aatcttcacg acggttggta gtgccattat gacggccatc ggggcgatta gctggccggt
     4501 tgtggccgtg gtggctgcca ttgtcgccgg ggcgttgctt atccgtaaat actgggagcc
     4561 tgtcagcgca ttctttggcg gtgtggttga agggctgaaa gcggcatttg cgccggtggg
     4621 ggagctgttc acgccactga aaccggtgtt tgactggctg ggcgaaaagt tacaggccgc
     4681 gtggcagtgg tttaaaaacc tgattgcccc ggttaaagcc acccaggaca ccctgaatcg
     4741 ttgccgtgac acgggcgtca tgttcgggca ggcactggct gacgcgctga tgctgccgct
     4801 taatgcgttc aacaaactgc gcagcggtat tgactgggta ctggaaaagc tcggggtcat
     4861 caacaaagag tctgacgcac ttgaccagac cgccgccaga actcaagccg ccacgtatgg
     4921 cagcggtagt tatattccgg cgaccagctc ttatgcaggc tatcaggctt atcagccggt
     4981 tacggcaccg gctggccgct cttatgtgga ccagagtaaa aacgaatatc acatcagtct
     5041 gacgggcggt actgcaccgg ggactcagct cgaccgccag ttacaggatg cgctcgaaaa
     5101 atacgagcgg gataaacgtg cgcgcgcccg tgccagcatg atgcatgacg gttaaggagg
     5161 tgacgaaaaa tgatgctcgc gttaggtatg tttgttttta tgcgccagac gctgccacac
     5221 cagaccatgc agcgtgaatc agattatcgc tggccgtcaa attcccgtat cggtaaacgg
     5281 gatgcctttc agtttctcgg tgtgggtgag gaaaacatta cgcttgccgg tgtgctttat
     5341 cccgaactga caggcggaaa gctgacgatg accacgctca ggctgatggc agaggaaggt
     5401 cgggcgtggc cgttgctgga tggcaccggc atgatttacg gcatgtatgt tatcagcagg
     5461 gtgagtgaaa cagggagtat tttctttgca gacggcacac cccggaaaat tgattttacg
     5521 ctgtcgctca cccgcgttga tgaatcactg gccgcgcttt atggcgatat cggtaaacag
     5581 gcggaatcgc tcatcggtaa ggctggcagt atggcgacca gattcacggg tatgacgggg
     5641 gcgggataat gctggatgcg ctgacatttg atgcaggcag tacgctgacg ccggattaca
     5701 tgctgatgct cgacagcagg gatattaccg gcaatatcag cgaccgtctg atgagcatga
     5761 ccctgacgga taaccggggc tttgaggctg accagcttga tattgaactg aacgatgccg
     5821 acgggcaggt cgggctgccg gttcgtggcg ctgtcctgac ggtgtatatc ggctggaaag
     5881 gttttgccct ggtatgcaaa gggaaattta ccgttgatga ggttgaacac cggggcgcgc
     5941 cggatgtggt taccatccgc gcccggagtg cagattttcg cgggacgctc aattcccgcc
     6001 gtgaaggctc ctggcatgac accacgctcg gtgcgattgt tgaggtgata gcctcccgta
     6061 acaggctgga agccagtgtc gcgccgtcac tggccggaat taaaatcccg cacatcgacc
     6121 agtcgcagga gtctgatgca aaattcctga cccgccttgc tgaacgcaac ggcggggagg
     6181 tgtcggtaaa aatgggaaaa ctgttgtttc tcaaagcggg gcagggagtg acggccagcg
     6241 gtaaaaaaat cccgcaggtc accataaccc gcagcgacgg cgaccgccat cattttgcga
     6301 ttgctgaccg tggagcctac accggtgtaa cggcaaagtg gttacacacc aaagacccga
     6361 agccgcaaaa gcagaaggta aaactgaaac gcaaaaagaa agataaacac ctgcgcgcac
     6421 tggagcaccc gaaagcgaaa ccagtcacgc agaagaaagc gccaaaagta ccggaagcgc
     6481 gcgaaggtga atatatggcc ggtgaggctg acaacgtttt tgccctgacc acggtatatg
     6541 ccacgaaagc acaggccatg cgcgccgctc aggctaagtg ggataaactg caacggggtg
     6601 tagcagagtt ctccatcagc ctggctaccg gtcgggccga tatttacacg gaaacaccgg
     6661 tcaaagtgtc tggctttaag cgcgtcatag acgagcagga ctggacaatc actaaggtga
     6721 cacattttct gaataatagc ggcttcacga cgtccttaga gcttgaggtc aggctttctg
     6781 atgtggagta cgaaacagaa gatgatgagt gatgtttttg ttttatctgt ttgttttgta
     6841 aggataaatt aactaaaatg gcaccatcaa caaaaccgga agaggtgctc gcgatgtttc
     6901 attgtccttt atgccagcat gccgcacatg cgcgtacaag tcgctatatc actgacacga
     6961 caaaagagcg ttatcatcag tgccagaacg tgaattgcag cgccacgttc atcacttatg
     7021 agtcggtaca gcgatacatc gtgaagccgg gagaagtcca cgccgtaagg ccgcacccgt
     7081 tgccatcagg acagcaaatt atgtggatgt aattacaaac agaaagcccc tcagttgagg
     7141 ggcttttttt gtcgatgtgg tcaatgtgtg gacgtgacca gaaataaatc cttttatttc
     7201 atgcagttag atatcaaaaa atgtcaccaa taactgacat tgcggtgttt ctccagatgt
     7261 ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct gttttggcag
     7321 tcaccttaaa gtatagatag ctgacaaaaa aggctctcgc gctaaaaaag gccccacttt
     7381 cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta attacataga
     7441 ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg agttgaaacg
     7501 tactgcgtag cc
//
LOCUS       DACAPB010000200         7438 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.201, whole genome shotgun sequence.
ACCESSION   DACAPB010000200 DACAPB010000000
VERSION     DACAPB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.201"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..774)
                     /locus_tag="IT904_004016"
     CDS             complement(<1..774)
                     /locus_tag="IT904_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAP3193720.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRECMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHAD"
     gene            925..2040
                     /gene="macA"
                     /locus_tag="IT904_004017"
     CDS             925..2040
                     /gene="macA"
                     /locus_tag="IT904_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAP3193721.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            2037..3983
                     /gene="macB"
                     /locus_tag="IT904_004018"
     CDS             2037..3983
                     /gene="macB"
                     /locus_tag="IT904_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAP3193722.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(4056..4280)
                     /gene="cspD"
                     /locus_tag="IT904_004019"
     CDS             complement(4056..4280)
                     /gene="cspD"
                     /locus_tag="IT904_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAP3193723.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            4603..4923
                     /gene="clpS"
                     /locus_tag="IT904_004020"
     CDS             4603..4923
                     /gene="clpS"
                     /locus_tag="IT904_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAP3193724.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            4954..7230
                     /gene="clpA"
                     /locus_tag="IT904_004021"
     CDS             4954..7230
                     /gene="clpA"
                     /locus_tag="IT904_004021"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAP3193725.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYETHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         1839 a         1723 c         2096 g         1780 t
ORIGIN      
        1 atcagcgtgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc
       61 attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac aggccgcttc
      121 catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct ggatttcctg
      181 atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc ttttcccctg
      241 atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt tgcggaacag
      301 gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa gcgtgaactg
      361 ctcgccgttt ttgccttcca gcttcgccag ttgcagcccg ggggtattca aatataagct
      421 gaattcttcc gccgacatac attcgcggag taacgcataa tggtaacgta acgcctccag
      481 caattgctta cggctaagat tcgccgcaag gtaagggcga tgcagacgca caggcagtcg
      541 cggctggcgc gttaacaata cattgagatt aggccagtgg gaaagttcgt tcatccactc
      601 aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa aactacggcg
      661 atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct
      721 gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat tcatgaaatc
      781 aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat caatagtgtg
      841 attactaacg taaattttag gggtttgttg atatttcgtt gaagttaatg acccggattg
      901 gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt
      961 attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct taacgcaccc
     1021 gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg
     1081 accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg
     1141 aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt aggggtcatt
     1201 gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat ggagctacgc
     1261 gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta ttcccgtcag
     1321 caacgtctgg cacaaacgca ggcggtttca cagcaggatc tcgacaccgc cgcgacggag
     1381 atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg caatcaggct
     1441 tctctcgata cggctaaaac caatctcgat tacactcgca tcgttgcccc gatggccggg
     1501 gaagtcacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca acaagcaccg
     1561 aacattctga cgctggcaga tatgagcacc atgctggtaa aagcgcaggt ttctgaagcg
     1621 gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtacttgg cgatccactg
     1681 acgcgctacg aggggcaaat caaggatgta ctaccgacgc cggaaaaggt taacgacgct
     1741 attttctatt acgcccgttt tgaagtcccc aaccccaatg gtttgctgcg gctggatatg
     1801 actgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgctgacgat ccctctgtcg
     1861 gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg taatggtgaa
     1921 acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga gattgtcaaa
     1981 gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc tgcacaatga
     2041 cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat gagcaggttg
     2101 aggtgttgaa gggcatcacc ctcgatattt atgctggtga gatggtggcg attgttggcg
     2161 cttcgggttc cggtaaatcg accctgatga atattctcgg ctgtctggat aaggccacca
     2221 gcggcaccta tcgcgtcgcc ggtcaggatg ttgccacgct ggacgccgat gcgctggcgc
     2281 aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt tcgcatttaa
     2341 ccgccgagca gaacgttgaa gttcccgccg tctatgctgg tcttgagcgg aaacagcggc
     2401 tgcttcgcgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca gagtattatc
     2461 cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca ttgatgaacg
     2521 gcggtcaggt aattcttgcc gatgaaccaa ccggcgcact ggacagccat tctggcgaag
     2581 aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt atcgtcaccc
     2641 acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac ggcgaaattg
     2701 tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag cctgttgtca
     2761 acacggtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg acgatggcat
     2821 ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg attattatcg
     2881 gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa atggtgctgg
     2941 cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa gattttggcg
     3001 atgacgatcc gcaatatcaa caggcgctga agtacgacga cttaatcgcc atccaaaaac
     3061 aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg cgttataaca
     3121 atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat gtctatggca
     3181 tgaccttcag tgaagggaac acctttaatc aggagcagct gaacggtcgt gcgcaggtcg
     3241 tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat gtggttggcg
     3301 aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt
     3361 cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg atgtccgggc
     3421 gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa ggatttgaca
     3481 gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacgga aagaaggatt
     3541 tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca cgtactttac
     3601 aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt ggtgtaatga
     3661 atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc atggctgtag
     3721 gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg gtttgcctgg
     3781 tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg cagcttttct
     3841 tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt ctctgctcga
     3901 cggtcaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga ctggatccag
     3961 tagatgctct ggcacgagag taatttttga gataaaaatg ccagccgatc gggctggcat
     4021 tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg
     4081 acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg
     4141 gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa
     4201 atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac
     4261 ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga
     4321 ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta
     4381 gagaattagg gcgagccgtc aagcatttga caggggacag ggggcaggta tgaatcaaaa
     4441 atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag
     4501 atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca
     4561 gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac
     4621 tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct
     4681 atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg
     4741 ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac
     4801 taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg
     4861 atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc
     4921 tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt
     4981 ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac
     5041 ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg
     5101 gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg
     5161 cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa
     5221 cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg
     5281 gtcgctatct ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc
     5341 agccgtctcg atgtggtgaa ttttatctct catggcacgc gtaaagacga gccgacacag
     5401 tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg
     5461 gagaatttca cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt
     5521 ggtcgtgaga aggagcttga gcgtgctatt caggttctct gccgtcgccg taaaaacaac
     5581 ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg tcttgcctgg
     5641 cgaattgttc agggcgatgt gccggaagtg atggctgact gtacaattta ctctctcgat
     5701 atcggttctc tgttagcggg cactaaatat cgcggcgact ttgaaaaacg ttttaaagcg
     5761 ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc
     5821 attatcggtg cgggtgcagc gtctggtggc caggtcgatg cggctaacct gatcaaaccg
     5881 ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac
     5941 attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg
     6001 tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga aacgcaccac
     6061 gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt
     6121 aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcggg cgctcgcgca
     6181 cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg
     6241 gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg
     6301 aaaaacctcg gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc cattgaggcg
     6361 ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt
     6421 tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg
     6481 aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc
     6541 gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg
     6601 actgatgcgg tcatcaagca tccacatgcg gttctgctgc tggacgaaat cgagaaagcg
     6661 caccctgacg tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac
     6721 aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta
     6781 cgtgaaactg agcgtaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg
     6841 gaagagatta agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg
     6901 tttgatcatc tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg
     6961 caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg
     7021 ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag
     7081 gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt
     7141 caggtcaccg tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca
     7201 caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtcaagtccg
     7261 taatctcgaa agaggttgcg gactttttat ttatggggtg gaggttcaga cccttttttt
     7321 aatgatgatg gtaagttgtt gataattagt gctgcgggaa ggtaaggata aaaaagggtg
     7381 ctgcaggaga atgggatggt tttgctttat taacaacggg ctaaacgtgt agtatttg
//
LOCUS       DACAPB010000201         7434 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.202, whole genome shotgun sequence.
ACCESSION   DACAPB010000201 DACAPB010000000
VERSION     DACAPB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.202"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(343..561)
                     /locus_tag="IT904_004022"
     CDS             complement(343..561)
                     /locus_tag="IT904_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193726.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(693..2216)
                     /locus_tag="IT904_004023"
     CDS             complement(693..2216)
                     /locus_tag="IT904_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP3193727.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2548..2796)
                     /locus_tag="IT904_004024"
     CDS             complement(2548..2796)
                     /locus_tag="IT904_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193728.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(2909..3175)
                     /gene="ariR"
                     /locus_tag="IT904_004025"
     CDS             complement(2909..3175)
                     /gene="ariR"
                     /locus_tag="IT904_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAP3193729.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(3204..3476)
                     /locus_tag="IT904_004026"
     CDS             complement(3204..3476)
                     /locus_tag="IT904_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAP3193730.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGWKINRIH"
     gene            complement(3519..3755)
                     /locus_tag="IT904_004027"
     CDS             complement(3519..3755)
                     /locus_tag="IT904_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAP3193731.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEKQKHYNALIGLLFE"
     gene            4069..5280
                     /gene="bluF"
                     /locus_tag="IT904_004028"
     CDS             4069..5280
                     /gene="bluF"
                     /locus_tag="IT904_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAP3193732.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            5485..6216
                     /locus_tag="IT904_004029"
     CDS             5485..6216
                     /locus_tag="IT904_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP3193733.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            6437..6841
                     /locus_tag="IT904_004030"
     CDS             6437..6841
                     /locus_tag="IT904_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAP3193734.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            6894..7004
                     /locus_tag="IT904_004031"
     CDS             6894..7004
                     /locus_tag="IT904_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193735.1"
                     /translation="MEIKVQRLSLWMINTVFLLSPINNHQTNTINLIFEM"
BASE COUNT         2282 a         1520 c         1457 g         2175 t
ORIGIN      
        1 tcagattacc aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata
       61 aacagcgaca atatataaat gataaataag tcggcattat catttttcac cttattttca
      121 tgacattgat cactttgagg tgacgatttt gtttaacaaa taactcttcg acatcaaaat
      181 tgttaatagt tgcgatgagg attatttcgg tgatatataa aaacaaccgt gataacacca
      241 gggttgacaa ctatttgcgc aataatgcct ttccttcaat gatccaggcg ttcactgcca
      301 gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg
      361 gttgacgagt ctttctctta atcgattaac cagattgatc gccagccaaa caagggcatt
      421 aagcaaattt atcgtcacaa gcacttctcc cagcccggta ctgggttttg caataaggat
      481 gaccaggagg gtgatcagca taaatgcaat ggaaaatatg atatagagag tgaaataatc
      541 cagattatta ctattgttca tgctactaat ggcaatcctg tcaaaacttt caaacaatta
      601 tagcaaacaa aataatgaac aatagtaaca agaggcaacg agctaattag ctgtctataa
      661 ttacaccgca ctatggcctt actgatcaat tttcactcaa ccacaacctt caccttcggt
      721 ttagaaagga taattttgac caggtcgatg tatgtaacag gtttatagaa ataataaccc
      781 tgctgaaatg tgatattatt ttggttcaga tagtcaagtt gttctttcgt ttcgacacct
      841 tcagcgacga tactgatcga aagtttacgc gccagatcca atacacaatc cagaattcgg
      901 gttgattctg ggtctgcgtt tacgcggcca acgaaactgt gatcaatttt gatataatca
      961 atatgcaggt catgaagata agaaagccct gagtagccag taccgaaatc atccagtgcg
     1021 atgacaaaac cattttcatg cagtatgttc aaccgctgaa ccagactttc atcaacattc
     1081 aatggctcac gctcggtgac ttccagaaca aggtttaaat cgcggcgggt aaaactgtca
     1141 cggaaattta aacactcgtc gacaaacgtc ggcgaaataa tatgcgaagc gctaaaatta
     1201 attccaatat gaaaaccttc cggcaattta ctcgcgatag cgttcatctg tctggcaacc
     1261 tgattcatca ggctttgcgt aagcggaacg attaatccgg atttttcagc aagtggaata
     1321 aatgccgcgg gtgatatata tccaccgtga ggttgtttcc agcgggctaa cacctcaact
     1381 ccccgcaatg tcccttcccg accatttacc acaggttggt aaaaagggac gatctcccca
     1441 ttatttattg cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa atatctatca
     1501 agcaggaagg cagcggcgca ggcgattaac agaataaata ttaaaatccc aaagccttta
     1561 tcgataagtc tgtagaagct aaagagtggc ggcgggttaa agataatagt gaaatgatat
     1621 tttgtggagt gaaccttctc ggtataggga taatgtccat taagcgtcat cacatcaccc
     1681 gttggtccaa taatgtcatc cgccacgcgt aatacatacc ttaccccttt caagggtaca
     1741 ttaagtgccc cacgaatatg ctgatcgctt atcgtaacca aaatgcgcgt atctgcaaat
     1801 tggttctgat agagcaggat aggtaatcta ttaacggtgt cgattgctgg agccaacagt
     1861 aaattactgt ccgggaaaac aggaatacga gacaacagga cccgattccc aggcagggat
     1921 gtacaccaga ctattccctg tttgagaatg atgattgtgc gcaggtgagg tttcagagct
     1981 gcttcagtgc caagctgata ttgctcctcg aggttgcatt ccttcccggc aatgttaacc
     2041 gctgtttgag tcgcttgtga cgcttcttca agtatatgat tgatattgtt ggcggcatat
     2101 ctcgctcccg ccaggtactc tgcttttgcg gaataccaga gctggatgtt caaaatagcg
     2161 acaccggtaa taaacaagca tatcgcaacg aggatgggta tgagtgtatt gcgcatttgc
     2221 ttgtcccggc caaagggttt aatacatgaa aatgccattc attcgctcgt gctggcatct
     2281 acagagagca tggtagagag cataagaaat atccacgccg aagataaaat tttctccatc
     2341 ttgatctaac ccattaatta ttgagcataa tgtaggcagt acaaaataag ttaggcggga
     2401 tatcaggcgt caagaatgga gagcgagaac tctccattct tgacacctga tattgcggac
     2461 ataataagaa agcataacgc ctgaaatgct cactttgtat cagcatggtg atacagctga
     2521 tgtttattct aaaaccttac tcaagttcta agagagcacg gattccctgt cattaataat
     2581 gatatccgtt agtgtagtct tatgcatttc aatgtactgc aatatttgca gatagagatc
     2641 agaaagaatc acactggttt catcttttaa acgcaagcgt aaagcctgat caataatatg
     2701 actatcaaca tacccatgcg tcatgaacac ctccctctcg agatttttaa acaaagcaca
     2761 ttgaaaaata aatctctctg ggattgaatt attcatgtca gcctcgtaga aatattaacc
     2821 taataaaatt atgctgtgta tagtctaaag cgacagacaa aaataagatg aatgagtttt
     2881 ctataaactt atacttaata attagaagtt acatatcatc agctgtgtat cgcaacacga
     2941 tttccagtgt ttttctaatc acatcagctt tgagaatatc acttgttgtt tccagggagt
     3001 gtatcagact taagataata tttttattat taacattatc gcctgaaagc attaaattgg
     3061 tgacagcctg ccctaacact gctgactctt cttctaacaa attacccgaa ctgcgaaagt
     3121 aacttgctaa cgctttatca gttattgcat tatgaattgt agtatcttca agcatggtaa
     3181 cctctcatct tacttatgaa attttaatgt attctgttta ttttccaacc attgaacata
     3241 cttttattgt cagctgtcgc tgttcttcgg tttggccctc ctgaatcact attgctaaaa
     3301 tcacgaatta tgctgtcaat taaatcgcgt cggaaagcat cttcctcagc cgatttcatt
     3361 acgagtaatt gcttaataag agtctgcaat gaaaaattta tcttcatgcg cagtatctgg
     3421 agcaccgcct ggccagtaat ttcatatttt atacgttcat tatcagatgt cttcataacg
     3481 acttcatagc taacctgtta aataatcagg ctgttgcatt attcaaaaag caacccaatt
     3541 agtgcattat aatgtttctg tttctcttcc cctgtcgctt gttccagtcg gcaaagaagt
     3601 ttggcacaaa gcgactttcg actcagatta cgtccatctt tcaaaatctc agtcacaatt
     3661 tccccgagag tttcctgctg agtaggcaag ttagcttttg caaagtaacg agtgattgct
     3721 cccgcagaat ctgaagtcac tgaattttga tgcatgctac gcctctgtta aaaatgttaa
     3781 tcaatgtgaa gtgaggtaac aatagcaaac ttgtacgaaa tatgtgtaca gaatgataaa
     3841 ttaatttttt gcaattagtg ctaatgcata taaaacaatg tgttatgata aaaaatattg
     3901 catcatttaa ctgacaatct atacaagatt tttcgccaac tagtgttcaa ctttttccat
     3961 tttttatcaa tgtaaagaaa cggttatgtt ttggtattaa aagaaagaca caagaagata
     4021 tgtctgttac cgtcttactc tcgcctcacc cattaccctg gattgttaat gcttaccacc
     4081 cttatttatc gtagccatat acgtgacgac gaacctgtca aaaaaatcga agaaatggtt
     4141 tcgatagcaa atcgcaggaa catgcagtct gacgtaacag ggatcttact gtttaatggt
     4201 tctcattttt tccagcttct ggaaggtccg gaagaacagg ttaaaatgat atatcgggct
     4261 atatgccagg atccacggca ctataatatt gttgagctgc tgtgcgatta cgcgcctgct
     4321 cgccgttttg gcaaagcggg aatggaatta tttgatttgc gcctgcacga gcgagatgac
     4381 gttttacagg ccgtattcga caaaggcaca tcaaaatttc agctaactta tgatgacaga
     4441 gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac aatcaaccta tttcgagatc
     4501 cctgccgaag actcctggct ttttatcgct gacggatctg ataaagaact tgattcctgt
     4561 gccctttcac caactataaa cgaccacttt gcctttcatc ctattgtcga tcccttatcg
     4621 cggcggataa tcgcttttga agccattgtg caaaaaaatg aagatagccc atcagccata
     4681 gcggttgggc agcgtaaaga cggggaaatc tacacagcgg atctcaaaag taaggcgctt
     4741 gcattcacga tggcacacgc acttgagctc ggtgataaaa tgatttcaat caatctatta
     4801 cctatgaccc tggttaacga acctgacgca gtctcttttt tacttaatga aataaaggcc
     4861 aatgctctgg tgcctgaaca aatcatcgtt gaatttactg aaagtgaagt catatctcgg
     4921 tttgatgagt ttgccgaagc gattaaatcg ctaaaggctg ctggtatcag tgtagcaatt
     4981 gatcattttg gcgcaggttt tgctggtttg ttactcctgt cacgcttcca gcctgacaga
     5041 attaaaatca gtcaggaatt gattaccaat gttcataaaa gtgggccacg gcaggcaatt
     5101 attcaggcga tcataaaatg ctgtacatca cttgaaattc aagtcagtgc tatgggcgtg
     5161 gcaacaccag aagagtggat gtggcttgaa tccgcaggaa ttgagatgtt tcagggagat
     5221 ctgtttgcta aagctaaatt gaatggtatc ccttccatag cgtggccaga gaaaaaataa
     5281 ttttcagcac attctttcac atgatttcag taaatcatcg tgaaaatgaa tacgttacgt
     5341 ttaaatttaa tccacattaa aaataatgtt tcatccaatt tagctgaaat cttatttata
     5401 ctttgtacaa ttttagcgta tattgtacaa agtattattg ggtcgtgtac aggcgacgga
     5461 gatttgtgac cgcaaggagg aattgtggct tattacagca ttggtgatgt tgctgaacgt
     5521 tgcgggatta atcctgtcac tctccgggcc tggcaacgcc gctacggttt gttaaaacca
     5581 cagcgcagtg aaggcggaca ccgactcttt gatgaagaag acatacaacg catcgaagag
     5641 atcaagcgtt ggataagtaa tggcgtccct gtaggcaaag ttaaagcatt actggaaacc
     5701 accagccagg atacggaaga tgactggagc cgcctgcaag aagagatgat gtcaattctt
     5761 cgcatggcta atcctgccaa actacgcgcg agaattattt cactgggtcg agagtaccca
     5821 gtagatcaat tgattaatca tgtttacctt cctgttcgcc agcgtctcgt gcttgatcac
     5881 aacacctccc gcattatgag cagtatgttt gacggcgcat taattgaata cgcagcaacc
     5941 tcgcttttcg aaatgcgccg taagcccggt aaagaagcca ttctgatggc gtggaatgtt
     6001 gaagagagag cacgattgtg gctggaagca tggcgtttat cattgtcagg atggcacatt
     6061 tctgttcttg ctgatcccat tgaatcgccg cgcccggaac tgttcccgac gcaaacattg
     6121 attgtctgga caggcatggc accaacgaga aggcaaaacg aacttttaca acactggggt
     6181 gagcaaggtt ataaagtcat ctttcatgcc ccctaatttc acctttatcc cgccccttag
     6241 acagaatgtt ataatctgaa cccacaacag cgtttgtacc ggagctggtc cgttgcaaaa
     6301 tgctcaccgg gagaattcag atattccctc tatcacactc tgtacatatc acagccaaag
     6361 gacagaccag attgaagtgg taaaaccgat cgtgcacact acgcagtctc acaactaaca
     6421 agaggttatt atcctgatgg atcaggtcgt tgtttttcaa aagatgtttg agcaagtgag
     6481 aaaagagcaa aacttctcct ggttttattc agaattaaaa catcaccgta ttgcacatta
     6541 catttattat ctggctacgg ataacatcag aattattact cacgatgata cggttttgtt
     6601 attaagagga accaggaacc tgttaaaagt tagtacgacc aagaaccctg ctaaaataaa
     6661 agaggccgca ttgcttcata tttgcggaaa atctacattt cgggaatact gttcaacact
     6721 ggcaggcgcg ggcgttttcc ggtgggttac tgatgttaat cataacaaac gcagttacta
     6781 cgccattgat aatacgcttt tatatattga agatgtagaa aataataaac cattaatcta
     6841 gctaaagttg gatacttaag aaatgcttca taattcagta aggcattagc ataatggaaa
     6901 taaaagtgca gagactatct ctatggatga ttaatactgt ctttttattg tcacccataa
     6961 ataatcacca gactaatact atcaacttga tatttgaaat gtgatcactt gacttttgat
     7021 acgttatttt ataacggtta acatatttat aaaaacaacg gccgtgccac acgtccgttt
     7081 caatacttaa cgcacatgta ttttggttta gtcatcatcc ggttatatgt attttagcca
     7141 agaacaggtt aaatcattcc tatataactc aaaaattgaa accttattct catgtcatgc
     7201 ttatattcat tattatcgtt atataaaaag gcaaccataa tgtttagcaa attggcacaa
     7261 agtagcataa aggctatgtt ttaattacag gatgttcagt catttgaatg tataacatta
     7321 tagctaaaca aatctaaaac gaagtcaata atttattgct ttcacaaaat ctcattttgt
     7381 ttaacatcca ttgagattcc ttgctttaaa ttttatttta tataagccat catt
//
LOCUS       DACAPB010000202         7358 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.203, whole genome shotgun sequence.
ACCESSION   DACAPB010000202 DACAPB010000000
VERSION     DACAPB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.203"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..650)
                     /gene="thiM"
                     /locus_tag="IT904_004032"
     CDS             complement(<1..650)
                     /gene="thiM"
                     /locus_tag="IT904_004032"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAP3193736.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDT"
     regulatory      complement(685..781)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(873..1145)
                     /gene="rcnR"
                     /locus_tag="IT904_004033"
     CDS             complement(873..1145)
                     /gene="rcnR"
                     /locus_tag="IT904_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAP3193737.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            1266..2090
                     /locus_tag="IT904_004034"
     CDS             1266..2090
                     /locus_tag="IT904_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAP3193738.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            2309..2647
                     /gene="rcnB"
                     /locus_tag="IT904_004035"
     CDS             2309..2647
                     /gene="rcnB"
                     /locus_tag="IT904_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAP3193739.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(2729..3763)
                     /locus_tag="IT904_004036"
     CDS             complement(2729..3763)
                     /locus_tag="IT904_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HAP3193740.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3779..6259)
                     /locus_tag="IT904_004037"
     CDS             complement(3779..6259)
                     /locus_tag="IT904_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP3193741.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDLAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGAGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQSLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(6275..6949)
                     /locus_tag="IT904_004038"
     CDS             complement(6275..6949)
                     /locus_tag="IT904_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP3193742.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKVLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(7029..>7358)
                     /locus_tag="IT904_004039"
     CDS             complement(7029..>7358)
                     /locus_tag="IT904_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3193743.1"
                     /translation="DDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSND
                     TVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSL
                     VMRVAQD"
BASE COUNT         1951 a         1792 c         1537 g         2078 t
ORIGIN      
        1 gtatcgcctg gtaacgcaca gcaggcagcg acaaccgccg ataatgcaca gccagttcct
       61 accactttgg tcattaacgg atcaccaccg tgaataccaa tgatacgatg tccatcggta
      121 acataatcca tctcgccagt gaccacgacg attgcgccag tttcccgtgc cagtgtttgt
      181 gcagcgggta tcgcgttagc tgcggcgtca gtggtatcca ctccccgtcc gccattagca
      241 atgccagcta atgccatgat ttccgaagca ttaccacgta tcgctgccgg tttaaaagat
      301 aaaagttcat gacaaaaatg gcggcgataa tcgagcgcac ccaccgctac tggatcaagc
      361 gtccagggtg tttgagagct ttttgcttgc tcaacggcag cacgcatcgc ctgagcgcgt
      421 ggctgcgtca gtgtgccaac gttaatcaac aaggcactgg cgatagccgc aaactgactg
      481 gcctcttcgg tttcgataac catcgctggc gatgcaccga gcgccagcaa ggtattggcg
      541 gtaaaggttt gcaccacatc attggtcatg cagtgcacaa gaggggaatg ttggtgaaaa
      601 aggtgtaacg cgtgcgcaga ttgcgctgaa cccagcaggt cgacttgcat agtttgctcc
      661 tgccataacg tgaagaagca atgacctggt ggtccgtgac ttccctacgc tggcattatc
      721 cagatcaggt gatacgggta tttctcagcc ttcacgcaga agggcacccc gagtcgtttg
      781 gttgcgatga taaatcgcag aggaggatgg taatgtccag cgcacgcgtt gtaaacgagg
      841 aaaaacggca gttttacaat cgcgttaata aattatttga tatatgaatc cagcaccttc
      901 agaacgacat ccagatcttc ttcacgtttt agctcatccc cctggtgaac gatgtgttcc
      961 gtcagatgac ctttaatcac ttcccgcatc agaccgttta ccgcgccacg gatagcagca
     1021 atctgttgta aaactgcagc gcattcgtgc ggctcgtcga gcattttctt gagcgccacg
     1081 acctggccct gaatcttact ggcacgcgct ttcagtttct gtttatcacg gattgtatga
     1141 gacatggcaa cacctggtta acaagaatat gaaaaatcat agcactatta atctactggg
     1201 gggtagtatc aggtactggg ggggagtaga atcagattgc cgaattaata ctaagaatta
     1261 ttatcatgac cgaatttaca actcttcttc agcaaggaaa cgcctggttc ttcatcccca
     1321 gcgccatctt acttggtgcg cttcatggcc tggaaccagg gcactcaaaa acgatgatgg
     1381 cggcgtttat catcgccatc aaaggcacca ttaaacaagc ggtgatgctc ggactggcag
     1441 caactatttc gcataccgca gtggtctggt taattgcctt tggcgggatg gtgatcagca
     1501 agcgctttac tgctcaatca gcagaaccgt ggctccagct gatttccgca gtgatcatta
     1561 ttagcaccgc gttctggatg ttctggcgta cctggcgcgg cgaacgcaac tggctggaga
     1621 atatgcacgg gcatgattat gagcatcatc atcacgatca cgaacatcac cacgaccatg
     1681 gacatcatca ccatcacgaa catggcgagt atcaggatgc ccatgcacga gcccatgcca
     1741 atgacattaa acgacgcttt gatggtagag aggtcaccaa ctggcaaatt ttgttatttg
     1801 gcttaaccgg tggccttatc ccctgcccgg cagcaattac cgtgctgttg atatgcattc
     1861 agttgaaagc cctgacactg ggcgcaacac tggtcgtcag tttcagcatt ggcctggcgt
     1921 taacgcttgt caccgtaggc gttggcgcag caatcagcgt tcagcaggtc gcaaaacgct
     1981 ggagcggatt taacactctc gctaaacgcg ccccctattt ttccagtctg ttgattggct
     2041 tagtcggtgt gtatatgggc gtacatggct tcatgggcat aatgcgataa ctcaatcctt
     2101 taggcttgca tgttatcttc agcccaggat gattcttaaa tcagctattt ccgctgactc
     2161 tctgctcgct aaggatgctt ttagggcatc ctttcattta cactttttac gaaatcatgg
     2221 gatcactaac aaaatatcgc ttgtcagtta tattgtatgg caggaaagat atgcgactga
     2281 tattacagat ccccaaagtg gagagtttat gaccattaaa aataagatgt tgctgggtgc
     2341 gcttttgctg gttaccagtg ccgcctgggc cgcaccagcc accgcgggtt cgaccaatac
     2401 ctcgggaatt tctaagtatg agttaagtag tttcattgct gactttaagc atttcaaacc
     2461 aggggatacc gtcccagaga tgtaccgcac cgatgagtat aacattaagc agtggcagtt
     2521 gcgtaacctg cccgcgcctg atgccgggac gcactggacc tatatgggtg gcgcgtacgt
     2581 gttgatcagc gacaccgacg gtaaaatcat taaagcctac gacggtgaga ttttttatca
     2641 tcgctaaaaa aagccccctc atcatgaggg ggaaatgcag acaccttgtt attttttatt
     2701 attagccact tgctcgtctt gcttgttatt agtcgtattt cacgttgatt aatgcggttg
     2761 cctccagtgc gccagattta actttgtttg tatcgtagac gtagtaactg gctgttatcg
     2821 gaattgaaac cgttgtatct gtaagttttg acttcgccgc aacttccttt aaatttgtat
     2881 atcctttact gctgttagtg atatttaaaa gtgtgactgg cgaaccatta ctttcaagaa
     2941 taaatcctac gccattatct tccgcacctt tcagtattgt gttgctgttg gttaacaagt
     3001 cagaacttga taaatacacc tgcattttcc gtgatgtttg attttgttga cctaataaat
     3061 ttttacaacg cagaataata tccccttgtt tactatttat tgttgctttg ttacctgtgg
     3121 ctggtaattg tgaaacggga atactgtcta aagtaattaa taaatcgtcc ggtttgcagg
     3181 ttgtggttat cgggttgtat gacaatgtca gattaaagga atagacacct gcattttgtg
     3241 ccaatgcatt tctgtagcta tttacacatg catcccatga cttgccacct aataaacaca
     3301 gtagaaattg tactgcggca ctcccgcgtg tcgatgatga agcactggat aatgtcgcaa
     3361 caccattaat catcactgaa ccgccgcccc ccgcagaaac attcactttt gcattactgg
     3421 ccgcaggtgt caccgtaact gaataaccac tggtgaaact ggtctgatta tttgacaacg
     3481 tgatgttgtt tttattggtc cattcaaaat gtaaatttaa ttttatggta tcgttataaa
     3541 gtccaacaat aatatccgac gggctcatag gggtgatagt atttgtactc acgcatgacg
     3601 aatcattaaa attatgctca tatgtcgcca tttgtgtttg agtaagtgca aaatccccct
     3661 gaacaaccaa gttgtgctga acagataact cactataaca tgccgcatag ctcacaggca
     3721 taaccatcat cattaatata aatagcatta tgcgaatttc catagaacca cctgactatt
     3781 actggcaaat ataatttcta ctttcatcaa tctctttacc gaaggtgatt gtgcatgaaa
     3841 gtccttgttg cttatcaatt gccacattaa ccgatggcgg tacttcattg gtgcgaataa
     3901 ataactgact tccctggccg acaacgccaa tattatgacc atggatatca ttgacttcat
     3961 aaccaaacgt taatgattgc ccatctgctc ttaacgcttt tataaaccat ggcttgcgct
     4021 gatcggtatc aaaattaacc agtacaaccg cgccgcgata aggggcggca attttccggt
     4081 tgccacgtaa ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg tgattttccc
     4141 gataaggtgt cattccgtcg tatatcacca ctccattacg gtttgttgtg cgatattttt
     4201 gcccattgac ataagcatct ttaattcctg gcgcattcat cacagcaaac gtttcggaaa
     4261 gacggttcgc cagattaacg ccacccgacc aggcgacaat gccccctgaa acactggctc
     4321 cagcctgtcg ataagtactc gactgactat aactgccatt cactgtcgca accggcgcgt
     4381 tccaggtcaa attcgcccca gctgtcgttt catttccctg atgttgatga ctcaggttga
     4441 caccataatt gaactgatcc cgactcccta ctgttcctga taatcccgta ttatttgagg
     4501 caaacccctg atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg
     4561 aaacgtcatc accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat
     4621 gatgattctc gtcataagcc tggcttgccg cgagggtata gcttatccgt cgcaggttgt
     4681 tggaataact caactgataa tccttactac tgccgctacg cccccagtaa tctcgccata
     4741 acgtacttaa tgacacagac ccccaacctt ctggcaatga ctggctcata ttggcggaaa
     4801 agctattttt gcggccaaaa tcgttctggt aataatcggc aatgtcatag acatcgtttt
     4861 catcacggcg ataattatct ttattgtttg cccaaacgtg atcgttaaat gtccggtaat
     4921 cacgcgacga ataacgccag gccgccagac caaaacgcgt cgacgtttgg ctcacaaatt
     4981 tgttgtaggc aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtttactat
     5041 gcgacttagt ggcatcgacg gaaatggcac caatgcgtgt attccagcca gcccccaaag
     5101 taaacgcgta ataattattc gcgaccatcg agccaccata cagcgtcaat aaattattaa
     5161 aaccatactg ataacccgcc tggacaaaat cactttgttt gctcgcccct tcaatatggc
     5221 tacgacccgc cgctaaatca tatttcgaca cgccgggttg cagcatattt ggcaccgctg
     5281 cataaggcac cagataggtg gttaccgagc cgtccgcctc tttcacgctg acatcaagat
     5341 ctgctccacc accggccaac tgcaaatctg taatcgcgaa cgggccagga ggaacctctt
     5401 tctgataaac cacaaaacca ttctgttcaa tagttaccag cgcgttactc tgagcaatcc
     5461 cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca
     5521 accgcacacc tctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca
     5581 cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc
     5641 acacccctgg attgttattt gttttactga aactggcatc agaatgcagt tgccacccca
     5701 gtaaatttaa cccgctgtta aaacgtacat atgtactctt gttattaccc gacgctttat
     5761 agtcgctgta atactgactc agataataag aggtataaaa cgcattaata ccccgctccc
     5821 agttttccgg tggaacatag ccactttcca gttcttccac ccaggcctgc gggacactga
     5881 aatcgagacg aaaaaccccg atatcccagg tatagctccc accctgaaca agttgctcaa
     5941 atgttaaaca ttgcttaccg ctggcgaagt tatcgctatt aatgcctaac cgcttgataa
     6001 cttctcttga taaacatgtt tcttgcgggt tgtctttaac aataatctca tatttcccgc
     6061 gccattgctt attgacataa atatcgatgt catactgccc cggtaagggt tgattatcat
     6121 caagacgaat atttgcaacc tgctggtctt tcattccacc tatcataaaa tgggtatcaa
     6181 aggtttcttc agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa
     6241 gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc
     6301 catggtcatc gataatggtc agatgccagt tatttgcatt attacttttg acattaacac
     6361 tctgactttc taagggggca atcataatag tttcataatt gaccttgaca ttattagctt
     6421 tgacatccga aatcgtcacc caattagctg agtcattttt tatcaccaaa ccattgccac
     6481 tgcgatttac cagcactttt ttaaatgtcg ctttatttac cggagcaata cccgctggcc
     6541 ggtaaaacaa cttaattctg ttttgcatcg caaacttcag tgcattctta ccttcttgct
     6601 ctggactatt tggtggaata tccagaacat tcagataaaa aatgctttct ttattagtgg
     6661 gcagtttatt cggcataatt ttgattttta cttgctgccc ggaatttgcc cctatttttg
     6721 ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaat gacgtatcgc
     6781 catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca
     6841 tcacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca attccggcat
     6901 gtgcaggaag gaccatagaa aaaatgagta aagataataa tcctttcatt ttaatgcctc
     6961 ttaaattaaa aggccgccat gggatagcgg ccatgtttat tacctggaga aacttacaat
     7021 tttatttatt aatcctgcgc cacacgcata accagagaag ataacacatc acctgattgc
     7081 actgagttat ctgtcagttt caggtatttt gctttaaacg gaatagcata ttgaccttcc
     7141 gctgttttat aactctcaat gttaaaaggt gcagaaacat ccaggacatc gctgttatca
     7201 cctaaataga gatagtaacc cacggtatcg ttgctactgg ctatcgcctt atcgttaccg
     7261 gttattttat cgttggtaat caacgagagt tttacaccag caacttcttt cgggcagcta
     7321 atttttaaca tgctgctggt tacaccgact gcatcatc
//
LOCUS       DACAPB010000203         7300 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.204, whole genome shotgun sequence.
ACCESSION   DACAPB010000203 DACAPB010000000
VERSION     DACAPB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.204"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..78
                     /locus_tag="IT904_004040"
     CDS             <1..78
                     /locus_tag="IT904_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP3193744.1"
                     /translation="SGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(65..757)
                     /gene="ygeA"
                     /locus_tag="IT904_004041"
     CDS             complement(65..757)
                     /gene="ygeA"
                     /locus_tag="IT904_004041"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAP3193745.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(886..2304)
                     /locus_tag="IT904_004042"
     CDS             complement(886..2304)
                     /locus_tag="IT904_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter"
                     /protein_id="HAP3193746.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
     gene            complement(2619..3380)
                     /gene="kduD"
                     /locus_tag="IT904_004043"
     CDS             complement(2619..3380)
                     /gene="kduD"
                     /locus_tag="IT904_004043"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAP3193747.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(3410..4246)
                     /gene="kduI"
                     /locus_tag="IT904_004044"
     CDS             complement(3410..4246)
                     /gene="kduI"
                     /locus_tag="IT904_004044"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP3193748.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASDDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(4533..5714)
                     /locus_tag="IT904_004045"
     CDS             complement(4533..5714)
                     /locus_tag="IT904_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAP3193749.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            5969..7198
                     /locus_tag="IT904_004046"
     CDS             5969..7198
                     /locus_tag="IT904_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP3193750.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSADEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1955 a         1921 c         1600 g         1824 t
ORIGIN      
        1 agtgggcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg
       61 tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgcg gtatcaaaca
      121 caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga
      181 cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg
      241 cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt
      301 catccgcttc aggaataaga caattgatgg aaaattgttc cgtcagccgc ccgcgataaa
      361 aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg
      421 taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa
      481 tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc
      541 gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac
      601 gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa
      661 gcccaccaag ccgctgttta atgccttcat ttatcaaacg atagtaagga atggtggatt
      721 cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgcgttac
      781 cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaagtgc ccgatctcta
      841 ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga tgccgatatt
      901 tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagggtga catttttggt
      961 ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca
     1021 gaacgtaccg gcagcgccaa tgctgtcgag cagcgtcagg aaggtcgcgc cgataatcat
     1081 attcgacacc cagttcgtgg tggtcgaaca ggtaataccg aaatcgcggc atttcagcgg
     1141 ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat
     1201 acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc
     1261 aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat
     1321 tttcagagcc ggtttacgcc ctgctttatc taccgtaaac accgcaataa aggtggcgaa
     1381 cataaaggtc agccctacga ccagagtcgc aatcatctgt tgttctgtcg tcgtaaagcc
     1441 cgccattttg aagatacgtg gcgcgtagta catgatgatg ttcataccgg taaactgctg
     1501 catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa
     1561 cagtgcccaa ccgccctgtt ttaacttcag gctttcacga atttcgttga gttcttctcg
     1621 cgctttttcc gacgtatcgc gcagcatacg caatacttct tccgcctcaa tatgacgccc
     1681 cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac
     1741 tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt
     1801 atcggataaa aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat
     1861 cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagaggc
     1921 gatcccgaca gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc
     1981 aaaagcggac cctatcgagc cgagcacaaa caggatggcc cccgccatca ggctgtattt
     2041 acgccccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat
     2101 catgctactg accacccatt cctgcaaacg gctggtcagc acaaagtgat cggtaatgaa
     2161 cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc
     2221 agctaccgaa acaaacatat tcatacgccg cgtatcccgc aaagaacgtg gcgttaaagc
     2281 agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa
     2341 aatacgtgaa caattcacac aggtgtcagg tcggaaatag cataaatatg gattaaattg
     2401 ctgcgacatg tcgttatgtg atggatattc caattttcaa attaagttga attattgaga
     2461 ttattattaa ccaactaatt ttaaagaaga taaaattcat aaagttcatt aattgataat
     2521 tgatatggat tatttcataa ccatgatatg gattatgatg atctacaggt ataaaaaacc
     2581 ctgccatgcg gcagggtcat aaaagtaaga agaatgaatt aacgcgccag ccaaccgcca
     2641 tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag gaacactacc
     2701 ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcggtc gagaatttcc
     2761 gcgctacgtt gttcatctgc ccgcagttgt tgagtattgt tggtcgccat gtaacccgga
     2821 gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa tcgcgtcacg
     2881 cccatcacac cgctttttga tgcggtataa gaaggcacac ggatcccgcc ctggaaggag
     2941 agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa gtgtttcgcc
     3001 gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc gtcccagtcc
     3061 ttttcgctga actcgagagc gtcttcgcgg cgaatcaatc cggcgttatt caccaggata
     3121 tcaatatgac caaattccgc taccgcgcga tccagcagtg ctggaatacc atcaatcttt
     3181 cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ctgtgacctg cttgatggtt
     3241 tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc cagccccaac
     3301 gccatcccct ggcccagtcc agtatcacaa ccagtgacga ccgcaacttt accttcgaga
     3361 gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgccgacaac tagcgcaaat
     3421 ctttaacggc cacatggtcc atatcatcaa agacctggtt ttcaccgacc atgccccaga
     3481 taaaggtata agctttggtt ccgacgccgg aatgaatcga ccagctcggg gagatcaccg
     3541 cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ctgccccatc atgtggaaaa
     3601 cgcaggcgtc atcatccata ttgaaataga aataaacttc catccggcgc tcgtgggtgt
     3661 gacacggcat ggtgttccac aagttacccg gagccagctc cgtcagcccc atactcaatt
     3721 ggcaggtttc cagcacatcc gggacaaaat atttgttaat agtgcgacgg ttactggtga
     3781 ggttatcgcc taacgtgact ggagatactt cgtccggggt gacttttttg gtgggatacg
     3841 tcgtatgcgc gggtgcgcaa ttgtaataaa acttcgccgg agtggcggta tcatcactgg
     3901 caaagacaac ttcctttgca cctttaccaa catacagggc gtcgcggtga ccgatttcat
     3961 agcattgacc atcgacagta atcgtaccgg caccgccaat attgataaca cctaactcgc
     4021 gacgttcaag gaaataactt acgccgagtt gtttacccac ttccccgcca acggaaaccg
     4081 ttttagttac cggcataatg ccgccaacaa taatacggtc aatgtggctg taaaccatgg
     4141 tgtactcatc ggcgacaaat acctcttcaa ccaaaaattc attgcgcagc ccttgggtat
     4201 ccagcgtttt tgcgtgcgca ctgtggatgc tctgtcttac gtccacatca acctccgata
     4261 atcagtgaag cgtgaaaata gaaaacgata gtgaaacact gtttcgtttt ctctataaag
     4321 ggatgatatg cccatgggct agggttttca attaaaaata aaacaatgtt tcatttttaa
     4381 tttaaggatt aaaaaaagtg cagtgtcgat cacgaatatg tcatgagaat ggaaaaatga
     4441 gggagcccag aaaatgtatg tcgacttgcc agccatcatt attcaacaga ttaatgacgg
     4501 cagatattta tcggtaagga ggaaatgcag agctactcgt cacgttcaat ggtcaatgcc
     4561 acgccctggc ccccgccgat acaaagcgtt gccagccctt tgcgggcatt acgtttcacc
     4621 atttcatgaa ccagagaaac caggattcgg caaccggaag cgcctatcgg gtgaccgagt
     4681 gcgatcgcgc caccattgac attgacccga cgctcatccc actcaagcat cttgccaacc
     4741 gaaagcgcct gtgcagcaaa cgcttcatta gcctcgataa gatcgacatc agccaactgc
     4801 cagcctacac gctccaggca acggcgggtc gcatacaccg gcgcaattcc catcaatgcc
     4861 ggatctacac caacgctggc aaatgcgcgg atgcgggcca gcacgggtaa attcaacgct
     4921 cgtgctttgg cttcgctcat catcattacc gcagctgcgc catcgtttat ggatgatgca
     4981 ttccccgcgg tcacagaacc gagactatca aatgaaggat ttaaacgggc taaaccttct
     5041 gcactggcgt cagtgcgtgg ctgttcatcg gtatcaacaa ccaacgtctg accgttactt
     5101 tggatcatta ccgggacgat ctcatcttta aatcgtccgg cgtcaatcgc cgctcgcgct
     5161 ttttgttgcg agctaagtgc gtaagcatcc tgcaactgac ggctgatgcc atattcgcga
     5221 gccagatttt cggcggtgac accaatatga taatcattga aggcatccca caacccatca
     5281 tgcaccagac tgtcaaccaa ctggctattg ccaagctgtg caccggtgcg gctatcagtc
     5341 agaacatgtg gtgcgcggct catgttttcc tggccaccgg cgatgacaat atcagcctcg
     5401 ccacactgta tcgcctgagt agccagatgc agtgccttaa gcccggaacc gcaaacgtca
     5461 ttaatcgtga ttgcagaaac gctattagga agaccacctt taatagccga ttgccttgcc
     5521 ggattctgcc ctgcccctgc agtcaacacc tggccaagaa tcacttcatc caccgcatat
     5581 gcaggcacgc cggtacgttc tattaacgct ttcacgacca ggctaccaag ttccacggcg
     5641 gaatgacccg ctaacgcacc acgaaagcag ccgataggtg tccgtaacgc cccgacaatc
     5701 acaacgtctt tcatcacaac ctcgcacaaa ttaacagtgc aatagtaaat gattgttaat
     5761 tacagttatc ttaattgttt aaaaaaagtg atttttatca caaaggaaat atgcctgagt
     5821 agcagtcaga gacataactg gcacgtaagg tttgcaacca ctaacccacc aatagagagg
     5881 tagatagggc gttaatctcc catacttaac ctggtttatg gtaaattgcc ctccattttg
     5941 tttaatttgt agatgatacg ttcagataat gtctaatatt tggtcaaaag aagaaactct
     6001 gtggagtttc gcgctctacg gcacagccgt tggtgcaggc acgctcttcc ttcctattca
     6061 gttaggttcg gcaggggcag tggtcctgtt tattactgct ctggtcgcct ggcctttaac
     6121 atattggcca cataaagcct tatgccagtt catcctctca tcgaaaacat cagcagatga
     6181 agggataacg ggcgcagtaa cacactacta tggcaagaag attggtaatc tgattaccac
     6241 gctgtacttc atcgcctttt ttgtcgtcgt gttgatatac gcagtggcaa ttaccaactc
     6301 acttacagaa cagctggcaa agcatatggt gattgatctt cgcatccgta tgttggtgag
     6361 tctgggtgtt gtattaatcc tgaatctcat ttttctgatg ggacgtcatg ccactattcg
     6421 ggtaatggga tttttggtat tcccattgat tgcctatttc ttatttcttt ccatttacct
     6481 tgtcggtagt tggcaacctg atctattaac aacccaggta gagttcaatc agaataccct
     6541 tcaccagata tggatatcga ttcccgtgat ggttttcgcc tttagccata cgcccattat
     6601 ttctacgttt gccatagaca gacgtgaaaa atatggcgaa cacgctatgg ataaatgcaa
     6661 aaaaattatg aaagtcgctt atctcatcat ctgcataagt gtactgttct ttgtctttag
     6721 ctgcctgctt tctattccac cttcgtatat tgaagcggct aaagaagaag gggtcaccat
     6781 tttatcggcg ctttctatgc tgccgaacgc cccagcatgg ttgtcaattt ccgggattat
     6841 tgtcgcagta gttgcgatgt cgaaatcatt cctgggtacg tactttggcg ttattgaagg
     6901 tgccacagag gtcgtcaaaa caacactaca gcaggttggt gtaaagaaaa gtcgtgcatt
     6961 taaccgcgca ctatcaatta tgttggtatc gctgattacc ttcattgttt gttgcattaa
     7021 cccgaacgcg atttcgatga tttatgcgat cagcggcccg ctcattgcca tgatactttt
     7081 catcatgcct acgctatcaa catatctcat cccggcgctt aaaccctggc gttccatcgg
     7141 aaatctgatt acgctgatcg tgggtatcct gtgcgtatcg gtaatgttct ttagctaata
     7201 tccattgtga atggttcaaa attgctacaa ttttgaacca ttccattttt cataatgttc
     7261 atttgttatt attccgccct atcacttctc tcatcatatc
//
LOCUS       DACAPB010000204         7138 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.205, whole genome shotgun sequence.
ACCESSION   DACAPB010000204 DACAPB010000000
VERSION     DACAPB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.205"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..449
                     /gene="rplB"
                     /locus_tag="IT904_004047"
     CDS             <1..449
                     /gene="rplB"
                     /locus_tag="IT904_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP3193751.1"
                     /translation="PGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDG
                     AYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNP
                     VDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            466..744
                     /gene="rpsS"
                     /locus_tag="IT904_004048"
     CDS             466..744
                     /gene="rpsS"
                     /locus_tag="IT904_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP3193752.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            759..1091
                     /gene="rplV"
                     /locus_tag="IT904_004049"
     CDS             759..1091
                     /gene="rplV"
                     /locus_tag="IT904_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP3193753.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            1109..1810
                     /gene="rpsC"
                     /locus_tag="IT904_004050"
     CDS             1109..1810
                     /gene="rpsC"
                     /locus_tag="IT904_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP3193754.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            1823..2233
                     /gene="rplP"
                     /locus_tag="IT904_004051"
     CDS             1823..2233
                     /gene="rplP"
                     /locus_tag="IT904_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP3193755.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            2233..2424
                     /gene="rpmC"
                     /locus_tag="IT904_004052"
     CDS             2233..2424
                     /gene="rpmC"
                     /locus_tag="IT904_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP3193756.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            2424..2678
                     /gene="rpsQ"
                     /locus_tag="IT904_004053"
     CDS             2424..2678
                     /gene="rpsQ"
                     /locus_tag="IT904_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP3193757.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            2843..3214
                     /gene="rplN"
                     /locus_tag="IT904_004054"
     CDS             2843..3214
                     /gene="rplN"
                     /locus_tag="IT904_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP3193758.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            3225..3539
                     /gene="rplX"
                     /locus_tag="IT904_004055"
     CDS             3225..3539
                     /gene="rplX"
                     /locus_tag="IT904_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP3193759.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            3554..4093
                     /gene="rplE"
                     /locus_tag="IT904_004056"
     CDS             3554..4093
                     /gene="rplE"
                     /locus_tag="IT904_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP3193760.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            4108..4413
                     /gene="rpsN"
                     /locus_tag="IT904_004057"
     CDS             4108..4413
                     /gene="rpsN"
                     /locus_tag="IT904_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP3193761.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            4447..4839
                     /gene="rpsH"
                     /locus_tag="IT904_004058"
     CDS             4447..4839
                     /gene="rpsH"
                     /locus_tag="IT904_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP3193762.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            4852..5385
                     /gene="rplF"
                     /locus_tag="IT904_004059"
     CDS             4852..5385
                     /gene="rplF"
                     /locus_tag="IT904_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP3193763.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            5395..5748
                     /gene="rplR"
                     /locus_tag="IT904_004060"
     CDS             5395..5748
                     /gene="rplR"
                     /locus_tag="IT904_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP3193764.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            5763..6266
                     /gene="rpsE"
                     /locus_tag="IT904_004061"
     CDS             5763..6266
                     /gene="rpsE"
                     /locus_tag="IT904_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP3193765.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            6270..6449
                     /gene="rpmD"
                     /locus_tag="IT904_004062"
     CDS             6270..6449
                     /gene="rpmD"
                     /locus_tag="IT904_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP3193766.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            6453..6887
                     /gene="rplO"
                     /locus_tag="IT904_004063"
     CDS             6453..6887
                     /gene="rplO"
                     /locus_tag="IT904_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP3193767.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            6895..>7138
                     /gene="secY"
                     /locus_tag="IT904_004064"
     CDS             6895..>7138
                     /gene="secY"
                     /locus_tag="IT904_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP3193768.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALG"
BASE COUNT         1832 a         1680 c         1942 g         1684 t
ORIGIN      
        1 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
       61 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
      121 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
      181 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
      241 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
      301 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
      361 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
      421 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
      481 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
      541 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
      601 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
      661 atggttggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
      721 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
      781 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
      841 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
      901 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
      961 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     1021 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     1081 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     1141 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     1201 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     1261 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     1321 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     1381 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     1441 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     1501 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     1561 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     1621 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     1681 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     1741 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     1801 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     1861 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     1921 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     1981 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     2041 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     2101 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     2161 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     2221 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     2281 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     2341 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     2401 ctgaacgaga aggcgggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     2461 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     2521 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     2581 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     2641 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcaatacg
     2701 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     2761 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     2821 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     2881 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     2941 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     3001 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     3061 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     3121 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     3181 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     3241 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     3301 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     3361 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     3421 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     3481 gattcgaaga cggtaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     3541 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     3601 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     3661 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     3721 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     3781 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     3841 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     3901 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     3961 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     4021 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     4081 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     4141 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     4201 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     4261 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     4321 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     4381 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     4441 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     4501 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     4561 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     4621 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     4681 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     4741 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     4801 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     4861 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     4921 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     4981 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     5041 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     5101 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg caatgtgatt
     5161 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     5221 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     5281 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     5341 tacgccgacg aagtcgtgcg taccaaagag gctaagaaga agtaaggtaa cactatggat
     5401 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     5461 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     5521 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     5581 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     5641 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     5701 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     5761 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     5821 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     5881 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     5941 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     6001 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     6061 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     6121 ttcataacgt tctggctaaa gcctatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     6181 ctattgatgg cctggaaaat atgaattctc cagaaatggt cgctgccaag cgtggtaaat
     6241 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     6301 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     6361 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     6421 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     6481 aaggctccaa aaaggcgggt aaacgcctgg gtcgtggtat cggttctggc ctcggtaaaa
     6541 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     6601 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     6661 aagcagcgat tacagccgaa attcgtctgt ctgacctggc taaagtagaa ggcggtgtag
     6721 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     6781 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     6841 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     6901 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
     6961 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
     7021 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
     7081 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctgggga
//
LOCUS       DACAPB010000205         7124 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.206, whole genome shotgun sequence.
ACCESSION   DACAPB010000205 DACAPB010000000
VERSION     DACAPB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.206"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1944
                     /gene="hrpA"
                     /locus_tag="IT904_004065"
     CDS             <1..1944
                     /gene="hrpA"
                     /locus_tag="IT904_004065"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAP3193769.1"
                     /translation="IFPGSGLFKKPPKWVMVAELVETSRLWGRIAARIDPEWVEPVAQ
                     HLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQIDPALCRELFIRHALV
                     EGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEFYDQRISHDVISARHF
                     DSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGNLKLRLSYQFEPGADA
                     DGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPVRRNFVPAPNYAEAFL
                     GRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKITFRVVDDKNKKLKEGR
                     SLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYEQKRGNYKVKAWPALV
                     DERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEKLPNKAKLGLYFNPYG
                     KVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELNDTVVDIAKQVEQILT
                     AVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFKRLGDTLRYLQAIEKR
                     LEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVKEIRWMIEELRVSYFA
                     QQLGTPYPISDKRILQAMEQISG"
     gene            2216..3016
                     /locus_tag="IT904_004066"
     CDS             2216..3016
                     /locus_tag="IT904_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP3193770.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHISHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            3213..4652
                     /gene="aldA"
                     /locus_tag="IT904_004067"
     CDS             3213..4652
                     /gene="aldA"
                     /locus_tag="IT904_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP3193771.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAEHAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVGGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(4694..5695)
                     /gene="gap"
                     /locus_tag="IT904_004068"
     CDS             complement(4694..5695)
                     /gene="gap"
                     /locus_tag="IT904_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP3193772.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            5884..6414
                     /gene="cybB"
                     /locus_tag="IT904_004069"
     CDS             5884..6414
                     /gene="cybB"
                     /locus_tag="IT904_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAP3193773.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            6659..6832
                     /locus_tag="IT904_004070"
     CDS             6659..6832
                     /locus_tag="IT904_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193774.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(6904..7053)
                     /gene="hokB"
                     /locus_tag="IT904_004071"
     CDS             complement(6904..7053)
                     /gene="hokB"
                     /locus_tag="IT904_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAP3193775.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1822 a         1641 c         1867 g         1794 t
ORIGIN      
        1 atcttccccg gttctggttt attcaaaaaa ccgcctaaat gggtaatggt ggcggaactg
       61 gtagaaacca gccgcctgtg ggggcgcatt gctgcgcgta tcgacccgga atgggtggag
      121 ccagttgctc agcatttgat taaacgcacc tacagcgaac cgcactggga acgggcgcag
      181 ggcgcggtga tggcaacgga aaaagtcact gtttatggtt tgccgattgt tgctgcgcgt
      241 aaggtcaact acagccagat cgatccggcg ttatgtcgtg aactctttat tcgccacgct
      301 ctggtggaag gtgactggca gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg
      361 gccgaagtgg aagagctgga acacaaatca cgtcgccgcg atattctggt tgatgacgaa
      421 acgctgtttg agttctacga ccagcgcatc agccacgatg taatctccgc tcgtcacttc
      481 gatagctggt ggaaaaaagt cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc
      541 atgttgatca aagaaggggc ggaaaaaatc agcaagctgg attacccgaa cttctggcat
      601 cagggcaatc tcaagctgcg tttgagctat cagtttgagc caggcgcaga cgcggacggt
      661 gtgaccgtac atattccgct gccgttactt aaccaggttg aggaaagcgg gtttgaatgg
      721 cagatccccg gtctgcgccg cgaattggtg attgctctga ttaaatcgtt gccgaaaccg
      781 gtacgccgta attttgtgcc tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca
      841 ccgctggagt taccgttgct cgacagcctt gagcgcgagt tacggcggat gaccggcgtt
      901 accgttgacc gcgaagactg gcactgggat caggtgcccg atcacctgaa aatcaccttc
      961 cgcgtggtgg atgacaaaaa caagaagcta aaagaagggc gctcgctaca agatctgaaa
     1021 gatgcgctga aaggcaaagt gcaggaaacg ctgtctgcgg tggcggatga cggtatcgag
     1081 cagagcgggc tgcatatctg gagttttggt cagctgccgg aaagctacga acagaagcgt
     1141 ggcaactaca aagtgaaggc gtggccagcg ctagtggatg aacgcgacag tgtggcgatc
     1201 aaactgtttg ataatccgct agagcaaaaa caggcaatgt ggaacggtct tcgccgtcta
     1261 ctgctgctga atattccctc gccgatcaag tatttacatg agaagttacc aaacaaagcc
     1321 aagctggggc tgtactttaa cccgtatggc aaagtgctgg agctgatcga cgactgtatc
     1381 tcctgcggtg tggataaatt gatcgacgcc aatggtggcc cggtctggac ggaagaaggc
     1441 tttgctgcgc tgcatgaaaa agtgcgtgcc gaactgaacg acacggtggt ggatattgcg
     1501 aagcaggtcg agcaaatcct tacggcagtg ttcaatatca acaaacgtct gaaagggcgg
     1561 gtggatatga ccatggcgct ggggctttct gacattaaag cgcagatggg cgggttggta
     1621 tatcgcggtt ttgtcactgg taacggcttc aaacggctgg gcgacacgct gcgatatttg
     1681 caggcgattg aaaaacgact ggaaaaactg gcggttgatc cacatcgcga ccgtgctcag
     1741 atgctgaaag tcgaaaacgt ccagcaggcg tggcagcaat ggatcaacaa actgccgcct
     1801 gcacgtcgtg aggatgaaga cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt
     1861 agttacttcg ctcaacaact tggtacgcct tatccgattt cagataagcg tattttgcag
     1921 gcgatggagc agattagcgg ttaaccctgc tatttgcctg ataaatctaa aacccggtaa
     1981 gcatttagcg ccgggttttt tattaattct aaaacggtaa ggacaaaaat tcaggaattc
     2041 agaaaaatac aattctcagc tgcaagatga ataatgttta tctacagcat ttccttaaaa
     2101 gatatgtcag gcttgcggag tggcggttaa ggaaatacga cttcctcctt tcagagtgct
     2161 ccgcttctca ctattatctc acgcagtatt cttaagggaa cgataaggag gaaccatgaa
     2221 cattaccccg tttccgacgc tttcgacggc aactatagat gccataaatg ttatcggaca
     2281 gtggctggcg caggatgatt tctccggtga ggtgccgtat caggctgatt gcgtcatact
     2341 tgcaggaaat gcggttatgc cgactatcga tgcggcatgt aagattgctc gcgatcagca
     2401 aattccttta ctgattagtg gtggtatcgg tcactcgaca acttttttgt atagcgccat
     2461 cgcacagcat ccgcactaca acactatccg caccactggc agagcagaag cgaccatcct
     2521 ggcggatatc gctcatcagt tctggcacat ttcgcatgaa aaaatctgga ttgaagacca
     2581 gtcaacaaac tgcggtgaaa acgcacgctt tagcatcgcg ctattgaatc aggccgtaga
     2641 acgagttcat acggctatcg ttgttcagga ccccaccatg cagcggcgca cgatggcgac
     2701 gttccgccgt atgactgggg acaatcccga tgcaccacgc tggttaagtt atcccggatt
     2761 cgttcctcag ttaggaaata acgcagacag tgtaatcttt gttaacccgt tgcaaggatt
     2821 atggccggtt gagcgttatc tatcactact cactggcgag ctgccgcgtt tacgcgatga
     2881 tagcgatggc tacggtcccc gcgggcgaga ttttatcgtt cacgttgatt ttccggcaga
     2941 agtcatccat gcatggcaaa cgctgaaaca cgatgcggtg cttatcgagg cgatggaaag
     3001 tcgctcgtta cgttaaaaat tgcccgtttg tgaaccactt gtttgcaaac gggcatgact
     3061 cctgactttt atttctgcct tttattcctt ttacacttgt ttttatgaag cccttcacag
     3121 aattgtcctt tcacgatgcc gtctctctga tgattgatgt taattaacaa tgtatttacc
     3181 gaaaacaaac atataaatca caggagtcgc ccatgtcagt acccgttcaa catcctatgt
     3241 atatcgatgg acagtttgtt acctggcgtg gagacgcatg gattgatgtg gtaaaccctg
     3301 ctacagaggc tgtcatttcc cgcatacccg atggtcaggc cgaggatgcc cgtaaggcaa
     3361 tcgatgcagc agaacatgca caaccagaat gggaagcgtt gcctgctatt gaacgcgcca
     3421 gttggttgcg caaaatctcc gccgggatcc gcgaacgcgc cagtgaaatc agtgcgctga
     3481 ttgttgaaga agggggcaag atccagcagc tggctgaagt cgaagtggct tttactgccg
     3541 actatatcga ttacatggcg gagtgggcac ggcgttacga gggcgagatt attcaaagcg
     3601 atcgtccagg agaaaatatt cttctgttta aacgtgcgct tggtgtgact accggcattc
     3661 tgccgtggaa cttcccgttc ttcctcattg cccgcaaaat ggctcccgct cttttgactg
     3721 gtaataccat cgtcattaaa cccagtgaat ttacgccaaa caatgcgatt gcattcgcca
     3781 aaatcgtcga tgaaataggc cttccgcgcg gcgtgtttaa ccttgtactg gggcgtggtg
     3841 aaaccgttgg gcaagaactg gcgggtaacc caaaggtcgc aatggtcagt atgacaggca
     3901 gcgtctctgc aggtgagaag attatggcga ctgcggcgaa aaacatcacc aaagtgtgcc
     3961 tggaattggg tggtaaagca ccagctatcg taatggacga tgccgatctt gaactggcag
     4021 tcaaagccat cgttgattca cgcgtcatta atagtgggca agtgtgtaac tgtgcagaac
     4081 gtgtttatgt acagaaaggc atttatgatc agttcgtcaa tcggctgggt gaagcgatgc
     4141 aggcggttca atttggtaac cccgctgaac gcaacgacat tgcgatgggg ccgttgatta
     4201 acgccgcggc gctggaaagg gtcgagcaaa aagtggcgcg cgcagtagaa gaaggggcga
     4261 gagtggcgtt gggtggcaaa gcagtaggcg ggaaaggata ttattatccg ccgacattgc
     4321 tgctggatgt tcgccaggaa atgtcgatta tgcatgagga aacctttggc ccggttctgc
     4381 cggtggtcgc atttgacacg ctggaagatg ctatctcaat ggctaatgac agtgattacg
     4441 gcctgacctc atcaatctat acccaaaatc tgaacgtcgc gatgaaagcc attaaagggc
     4501 tgaagtttgg tgaaacttac atcaaccgtg aaaacttcga agctatgcaa ggcttccacg
     4561 ccggatggcg taaatccggt attggcggcg cagatggtaa acatggcttg catgaatatc
     4621 tgcagaccca ggtggtttat ttacagtctt aatgagtgaa agaggcggag gttttttcct
     4681 ccgcctgtgc gcgtcagagt ttagcgaatt tttcgagggt gcgaataagc tgtgtgacga
     4741 agccatattc gttatcgtac caggcgaccg ttttcaccag ttgtaaatcg cccacggcgg
     4801 taatttccgt ttgcgtggca tcaaacaccg aaccgaaatg gctgccaatg atatcggaag
     4861 agactatttc ttcatcggta taaccaaatg actcgttatt ggtggttgct tgtttaagtg
     4921 cgttattcac ctcttcggca gtcacttttt ttccgagaat cgataccagt tcagtgaccg
     4981 aacctgtttt caccggcacg cgttgcgcat gacctttcag tttgccgctc agttccggga
     5041 tcaccagacc aatggctttt gccgcccccg tagtgtgggg aatgatattt tctgccgctg
     5101 cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac cagtgactgg gtgccagtat
     5161 aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa actgtcatgc aaggctttgg
     5221 ccatcggcgc aagacagtta gtggtgcatg acgccacgga aacaatggtg tcgttgccat
     5281 ccagagtgtc gtcattgacg ttataaacga tagttttcat ttcaccggca ggggcggaaa
     5341 tcaacacctt cttcgcacca gcatcaagat gcgcctgcga tttctcggcg gaggtataaa
     5401 agccagtaca ttcgacaatg atttctgcac ctttcgcttt ccacggaata tttttagcct
     5461 ctttttcggc gtaaaccgcg atacttttcc catcaacgat aagtgaatct tccgtaaaat
     5521 caacgctcca ggggaatggt ccgtagtttg aatcatgttt cagcaggtag gcgagaattt
     5581 ttggggaagt gagatcatta atagcgacaa cgtctatgtt gcttttgact tcaagtaatc
     5641 gacgcaacac cagtcgaccg atacgaccaa aaccgttaat accaacttta ctcatggttt
     5701 tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc aggcttagac caggatggcg
     5761 ggatgggcaa tctccattct cacagtgaaa cgtaacgtac tgaaaacggg tgaacaatat
     5821 ttaatgaaat tttgagaaaa gcccgttatg ttaacggaaa attatgttaa agcaggaaat
     5881 gttatggaaa ataaatattc aaggttacaa atcagcattc actggctggt ctttttactg
     5941 gttatcgcag cgtattgcgc aatggagttt cgtggtttct tcccacgtag cgatcggcca
     6001 ctcatcaaca tgattcatgt ttcctgtggc atctcaattc tcgtgctgat ggtcgttcgt
     6061 cttttgttaa ggctgaaata cccaaccccg ccgattatac ctaagccaaa accgatgatg
     6121 acgggactgg cgcatttggg acatttggtg atttatctcc tgtttattgc gctgccagtg
     6181 attggtttgg tgatgatgta taaccggggc aacccgtggt ttgcgtttgg tttgacgatg
     6241 ccttacgctt cagaggccaa tttcgaacgg gtagatagct taaagtcgtg gcatgaaacg
     6301 ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg cggctgcact ggcacaccac
     6361 tatttctgga aggacaacac acttctacgc atgatgccgc gtaaacgttc ctgaaggata
     6421 tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat acgggtctac
     6481 atcggaagcg cctattatat ttatttgtat gacaaataaa accataatcc ttgcccatac
     6541 gtccatctgg cttattttta atcaattcac ccgatctttg atctcatcaa cggtatcaaa
     6601 ataaaccagc gtataacgtt caaatcgaac ataagattcg ataaaccatg gaggttatat
     6661 gaaaaaactg gcacttattt tgtttatggg aacgcttgtt tccttttatg ccgatgccgg
     6721 gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac tgtacggcag gcggcaaatt
     6781 tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca tgcactaact gaagtgtaaa
     6841 aggggtgcca tgagataatg aatctgctag tcagatgcgc ggggaaaatc cccgcgcttg
     6901 cccttacctg gacgtgcagg ccatgagcgc agcaacctcc ttatcaccgt cccggaaccg
     6961 cagttcgtag agcgtttgtc gggtcaggag tgtgaatgtc agaatcgtaa tgcagataat
     7021 gagcagacac accaccagag ggttgcgttt catagccttt ctccttgccg gatggcgggt
     7081 aagaggctaa gatctgaatt gctatgttca ttcgttggcc tcgg
//
LOCUS       DACAPB010000206         7119 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.207, whole genome shotgun sequence.
ACCESSION   DACAPB010000206 DACAPB010000000
VERSION     DACAPB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.207"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..869
                     /gene="malY"
                     /locus_tag="IT904_004072"
     CDS             <1..869
                     /gene="malY"
                     /locus_tag="IT904_004072"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAP3193776.1"
                     /translation="LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADG
                     WFCDMGKLEAVLAKPECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIH
                     MDMVWGEQPHIPWSNVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKG
                     RNGLSSPSVLALTAHIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQS
                     TYLAWLDLRPLNIDDNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKG
                     VAGLINAIRAVR"
     gene            973..1974
                     /gene="add"
                     /locus_tag="IT904_004073"
     CDS             973..1974
                     /gene="add"
                     /locus_tag="IT904_004073"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP3193777.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(2008..3048)
                     /locus_tag="IT904_004074"
     CDS             complement(2008..3048)
                     /locus_tag="IT904_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP3193778.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            3291..3416
                     /gene="blr"
                     /locus_tag="IT904_004075"
     CDS             3291..3416
                     /gene="blr"
                     /locus_tag="IT904_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAP3193779.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            3689..3904
                     /gene="ydgT"
                     /locus_tag="IT904_004076"
     CDS             3689..3904
                     /gene="ydgT"
                     /locus_tag="IT904_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAP3193780.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            3990..4430
                     /locus_tag="IT904_004077"
     CDS             3990..4430
                     /locus_tag="IT904_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAP3193781.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            4507..5088
                     /gene="rsxA"
                     /locus_tag="IT904_004078"
     CDS             4507..5088
                     /gene="rsxA"
                     /locus_tag="IT904_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAP3193782.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            5088..5666
                     /gene="rsxB"
                     /locus_tag="IT904_004079"
     CDS             5088..5666
                     /gene="rsxB"
                     /locus_tag="IT904_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAP3193783.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            5659..>7119
                     /gene="rsxC"
                     /locus_tag="IT904_004080"
     CDS             5659..>7119
                     /gene="rsxC"
                     /locus_tag="IT904_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAP3193784.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAA"
BASE COUNT         1645 a         1805 c         1822 g         1847 t
ORIGIN      
        1 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacaca cccgcctatg
       61 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
      121 agcaggctga tggttggttt tgcgatatgg gcaagttgga agccgtgttg gcgaaaccag
      181 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
      241 atgagctgga gatcatggct gacctgtgcg agcgtcatgg tgtgcgggtt atttccgatg
      301 aaatccatat ggatatggtt tggggcgagc agccgcatat tccctggagt aatgtggctc
      361 gcggagactg ggcgttgcta acgtcgggct cgaaaagttt caatattccc gccctgaccg
      421 gtgcttacgg gattatagaa aatagcagta gccgcgatgc ctatttatcg gcactgaaag
      481 gccgtaatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
      541 agcaaggcgc gccgtggctg gatgccttac gcatctatct gaaagataac ctgacgtata
      601 tcgcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatccca caatccactt
      661 atctggcatg gcttgattta cgtccgttga atattgacga caacgcgttg caaaaagcac
      721 ttatcgaaca agaaaaagtc gcgatcatgc cggggtatac ctacggtgaa gaaggtcgtg
      781 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
      841 taattaacgc catccgcgct gttcgttaac cccaattgcg caacgtaaaa aatcgttgcg
      901 caatcgtgga tttttaccct gctttgtttt tataatggtg cgcactttta tatccagaaa
      961 aagagtgcga ccatgattga taccaccctg ccattaactg atatccatcg ccaccttgat
     1021 ggcaacattc gtccccagac cattcttgaa cttggccgcc agtataatat ctcgcttcct
     1081 gcacaatccc tggaaacact gattccccac gttcaggtca ttgccaacga acccgatctg
     1141 gtgagctttc tgaccaaact tgactggggc gttaaagttc tcgcctctct tgatgcctgt
     1201 cgccgcgtgg catttgaaaa cattgaagat gcagcccgtc acggcctgca ctatgtcgag
     1261 ctgcgttttt caccaggcta catggcaatg gcacatcagc tgcctgtagc gggtgttgtc
     1321 gaagcggtga tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca ggcgaagctt
     1381 atcggcatta tgagccggac cttcggcgaa gccgcctgtc agcaagagct ggaggccttt
     1441 ttagcccacc gtgaccagat taccgcactt gatttagccg gtgatgaact tggtttcccg
     1501 ggaagtctgt tcctttctca cttcaaccgc gcgcgtgatg cgggctggca tattaccgtc
     1561 catgcaggcg aagctgccgg gccggaaagc atctggcagg cgattcgtga actgggtgcg
     1621 gagcgtattg gacatggcgt aaaagccatt gaagatcggg cgctgatgga ttttctcgcc
     1681 gagcaacaaa ttggtattga atcctgtctg acctccaata ttcagaccag caccgtagca
     1741 gagctggctg cacatccgct gaaaacgttc cttgagcatg gcattcgtgc cagcattaac
     1801 actgacgatc ccggcgtaca gggagtggat atcattcacg aatataccgt tgccgcgcca
     1861 gctgctgggt tatcccgcga gcaaatccgc caggcacaga ttaatggtct ggaaatggct
     1921 ttcctcagcg ctgaggaaaa acgcgcactg cgagaaaaag tcgccgcgaa gtaacaaaat
     1981 ggatggtgca aatgcaccat ccatttttca tgcaaggcac aaagtcgcgc gatgtttggc
     2041 ggattcgatg cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg
     2101 attttcacca tcgccattta acgcatcacg aatagccgca tagtaagccg gataattccc
     2161 aggcaccgtc aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc
     2221 atcacgcata tcgtagcccc agtcttcctg cggtagacgc tcgccatttt tcagacgttc
     2281 ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg
     2341 tgctgactca gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga
     2401 caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac
     2461 cgtcatgctg accggtaaac caaaaagcgt aatggcctga tcaagaagat gtggtgctaa
     2521 atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg
     2581 acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag
     2641 taaacctttt agcgtcaaga aatcgctatc ccagcgacgg ttatggaata cagacagcac
     2701 acgccccagg ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa
     2761 gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg
     2821 ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt
     2881 cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct
     2941 ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt
     3001 gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct
     3061 cattgaagta tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc
     3121 tgccagcaat aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac
     3181 aaaagctaaa tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta
     3241 acatttaccc cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc
     3301 ttattgaatt aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga
     3361 gtcacattga caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc
     3421 tgcacttgtg gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa
     3481 accaggaatt tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt
     3541 tactcttttc agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt
     3601 gtaataacac aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct
     3661 ttggtattaa ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg
     3721 caaaatcagt tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac
     3781 ggacgatcag gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt
     3841 ttctggcggg cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca
     3901 ataaagaaag tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg
     3961 cctgtttgcg tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag
     4021 gctggttgct tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt
     4081 tgttgctgta taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag
     4141 cactgacgac acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt
     4201 acacactgtg gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata
     4261 ttatctggct gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg
     4321 aagatggcat agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc
     4381 cctatttcaa acgctcgtcg cgggtaaaag cgacgtttgt aaatccgtaa taaccttaca
     4441 gttaacctgt tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc
     4501 cgaaaaatga ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta
     4561 ctggtcaagt ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg
     4621 atgggcatgg ggctggcaac aacgtttgtg atgacgctgg cgtctatttg cgcctggctt
     4681 atcgatacgt ggattttgat cccacttaat ctgatttacc tgcgcaccct ggcatttatt
     4741 ctggtgattg ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg
     4801 ctttaccgtc tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc
     4861 gtggcgttgc tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt
     4921 tccgccgctg tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt
     4981 gctgtggctg atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt
     5041 cttatgtctc tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct
     5101 ggattgccgt tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg
     5161 cctcccgccg ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac
     5221 cgcagagcca gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca
     5281 gctgtaacgg tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa
     5341 ttgccgagtt gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagataacgc
     5401 ctgcgcggat ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc
     5461 aggcgtgtcc ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg
     5521 atctctgtac gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc
     5581 aaccggtcgc agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta
     5641 tcattcccgt ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg
     5701 gatttcaacg gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacacccctg
     5761 cgccaggtac ccctggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa
     5821 ggtgagttgt gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt
     5881 cgcggcaaaa tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc
     5941 cactctacgg ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt
     6001 gaagactgct ggatcccgcg cgacggctgg gccgattatc gcactcgcag tcgcgaagag
     6061 ttaatcgagc gcatacatca gtttggtgtt gccgggctgg gcggtgcagg attcccgaca
     6121 ggcgttaaat tgcagggtgg cggagataag attgaaacgt tgattatcaa cgcggctgag
     6181 tgcgagccgt acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta
     6241 gagggtattc gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa
     6301 gataacaaac cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt
     6361 tctctgcggg tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt
     6421 ctgaccggga agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa
     6481 aacgtcggca ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag
     6541 cgtgttgtaa ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg
     6601 gggacgccag tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg
     6661 gtgattatgg gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta
     6721 aagattacca actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa
     6781 caaagctgca tccggtgtag cgcctgtgct gacgcctgcc ctgcggatct tttgccgcaa
     6841 cagttgtact ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgct
     6901 gattgcattg aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cctggtgcaa
     6961 tatttccgtc aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca
     7021 gaagccaaag cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc
     7081 cttgaacgac ataagagcgc agccgttcaa cctgcagcc
//
LOCUS       DACAPB010000207         7044 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.208, whole genome shotgun sequence.
ACCESSION   DACAPB010000207 DACAPB010000000
VERSION     DACAPB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.208"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            49..825
                     /locus_tag="IT904_004081"
     CDS             49..825
                     /locus_tag="IT904_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IV"
                     /protein_id="HAP3193785.1"
                     /translation="MQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGL
                     IEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPF
                     AFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMA
                     KVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARL
                     IRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     gene            1054..2079
                     /gene="aph(4)-Ia"
                     /locus_tag="IT904_004082"
     CDS             1054..2079
                     /gene="aph(4)-Ia"
                     /locus_tag="IT904_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /protein_id="HAP3193786.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE"
     gene            2085..2450
                     /locus_tag="IT904_004083"
                     /pseudo
     CDS             2085..2450
                     /locus_tag="IT904_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
     gene            complement(2501..3253)
                     /locus_tag="IT904_004084"
     CDS             complement(2501..3253)
                     /locus_tag="IT904_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP3193787.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRD
                     LEEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRY
                     LYRAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKT
                     SVDDGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLW
                     LRKGFGFSGGWTVNDQNDLLARLFGLQKVNKA"
     gene            3339..4838
                     /locus_tag="IT904_004085"
                     /pseudo
     CDS             3339..4838
                     /locus_tag="IT904_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4908..5783
                     /locus_tag="IT904_004086"
     CDS             4908..5783
                     /locus_tag="IT904_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HAP3193788.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(5937..6752)
                     /locus_tag="IT904_004087"
     CDS             complement(5937..6752)
                     /locus_tag="IT904_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HAP3193789.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT         1550 a         2008 c         1978 g         1508 t
ORIGIN      
        1 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
       61 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
      121 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
      181 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
      241 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
      301 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
      361 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac
      421 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
      481 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
      541 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
      601 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
      661 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
      721 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
      781 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
      841 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat
      901 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc
      961 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg
     1021 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
     1081 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     1141 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     1201 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     1261 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     1321 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     1381 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     1441 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     1501 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     1561 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     1621 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     1681 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     1741 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1801 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1861 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1921 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1981 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     2041 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag
     2101 agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag
     2161 agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct
     2221 gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt ggtccccagg
     2281 gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat tggagatcgc
     2341 cgcccgtgcc tgccgattgg gtgcagatct tgcgcaacga gatgccggca tacgatccgc
     2401 aaaccacacc aactcggaac atgggtgcag tggctgaatt gttccgggcg tgtcgcaaac
     2461 acttttggag gcagctatca cccagccaag gccggtattt tcatgctttg ttaacctttt
     2521 gcagtccgaa gaggcgcgca agcaaatcat tctggtcatt gacggtccag ccgcctgaaa
     2581 agccaaagcc cttccgcagc cacagcatcg cctcgaaacc cgcgatggtt cgccgcgccg
     2641 tgttaaagga ttggaaaccg ccgatcttcg gcatgttctt cttcacccgg aaatggtcgc
     2701 tctcaatccc ctgctggaga tgtttggtca cataatgcac gggatcggga tggagaagcc
     2761 catcatcaac cgacgttttg atcgttgacg ggaaggtgtt ggccccgtcc gtcccaatcc
     2821 tgttcggcga caacaagggt tcatctttaa gcatctttcg gaagaaccgc ttggcagcgt
     2881 cgagatcgcg cttagcggtc agcaggaaat ccaccggatt gccatgctta tcgatggctc
     2941 ggtacagata gcgccatttg ccgcggatct tgacataggt ctcatcaatc cggaccgagc
     3001 cgcaatgggg tcgacgaaac tgccgcagcc gtttctcgat gacaggtgca taagccaata
     3061 cccagcggtt gatcgtgcta tgatcgacct cgaagccccg ctcgcgaaac atctcttcca
     3121 agtcacgata gctgagcggg tagcgcaagt accaggcaac cgcctgtaca atcagccagg
     3181 cctcgaaatg cctgcccttg aaatcatcct tcgactggcg cttcagcttt tcggcaatgg
     3241 tattcaaaat catcggctcg ctccgcaata tcaggagcgg caacttctct ccccatggtc
     3301 aacatcgagt taacctgaag aatttgcgac aaggccatgc gttccgctcc ggagatgtct
     3361 ggcttactag gtcccggcgc tatggcgatc tgaaacacgc actcgttccg gcacaatcca
     3421 tcgcggaagg cggtcgtctc gctgtgccat tgcggccgga ggaatggctg gcagaccggc
     3481 aagctcgcct cgacatgcgg ttgcgcgagc ttggccgtgc cgctcgcgca ggcacgatcc
     3541 cgggcgggtc gattgaaaac ggcgttctgc atatcgagaa actcgaagcc gccgcgccga
     3601 caggcgccga agatctggtg ctcgatctct acaagcagat cccgcccacg cgcatcaccg
     3661 atctcctgct ggaggtggat gcggcgaccg gcttcaccga agcgttcacc catctgcgca
     3721 caggagcacc ctgcgctgac cggatcgggc taatgaacgt tatcttggcg gaagggatca
     3781 acctcggctt gcgcaaaatg gcggatgcga caaacaccca caccttctgg gaattgatcc
     3841 gcattggacg gtggcatgtc gagggcgaag cctatgaccg ggcgctggcc atggtggtcg
     3901 aggcacaggc agcgttaccc atggcccggt tctggggcat gggcacgtcg gcttcgagcg
     3961 acggacagtt cttcgtcgct acagagcaag gtgaggccat gaacctggtc aacgcgaaat
     4021 atggcaatac cccgggcctg aaagcctata gccacgtctc cgaccaatat gcgccgttcg
     4081 caacccaggt gattcctgca acggcaagcg aagcgcctta catcctcgat ggcctgctga
     4141 tgaacgatgc tggacgccat atccgcgagc agttcaccga cacgggcggc ttcaccgatc
     4201 acgtctttgc cgcatgtgcc attctcggct accggttcgc tccgcgcatc cgcgacctgc
     4261 catccaaacg gctctacgcg ttcaatccgt cggccgcccc ggcgcacctg cgagcgttga
     4321 tcggcggaaa ggtcaaccaa gccatgatcg agcgcaattg gcccgacatc ctgcgcatcg
     4381 ccgccaccat tgctgccggg accgtcgcgc caagccagat tctgcggaaa ctcgcctcct
     4441 atccgcggca gaacgagctc gcgacagccc tgcgggaagt cggtcgcgtc gagcgcaccc
     4501 tgttcatgat cgactggatt ctggatgccg aactccaacg gcgtgcccag atcgggctca
     4561 acaaaggcga agctcatcat gcgctgaagc gggcaatcag cttccaccgc cgcggtgaaa
     4621 tccgcgaccg ttccgccgaa ggccagcatt accgcatcgc cggcatgaat ctgctcgccg
     4681 ccatcatcat cttctggaac accatgaagc tcggcgaggt cgttgcaaac cagaaacgcg
     4741 atggaaagct gctatcgccc gatctcttgg cccatgtttc gccgctcgga tgggaacaca
     4801 tcaatctcac cggagaatat cgctggccaa agccttagcg taggattccg ccccctcccg
     4861 caaacgaccc cgtgatgacc ctgcaggcat cccctgagac ccggttaatg cgcgccgagg
     4921 tgcagcggca ttatccgcac atctggcagg agctggaggg gctggcgcag ggactggagg
     4981 ccactgttga tcaggtcttt gcctggaact gccgtggcga tctggtgcgc tcaacgtcgg
     5041 atggctgtac gacggtggcc ggggtaaacg ccgcaggcga gcggattatc gcgcataatg
     5101 aagacggttt tcctcagtta cgggatgaat gcttgctggc taccattact ccgagtcgcg
     5161 gtctgagctt tacgagtttt gcctatccgg ggtcgatttg cggccacacc tttgcggtaa
     5221 atgaaaaagg tatcgttaac acggtgaata acatccgtgc agtgtatcgt ccgcagggat
     5281 taccgcgtca gatcctggct cgcgcctcgc tgaatgcgaa caccctcgac gaggccatca
     5341 ctatacttac cggccagccg cgatccgggg cgtttcacca tacgctcggg cagctgggcg
     5401 atgcgcggct gttcagcgtt gaggccaccg gtcaaggctg ttccgttcta cccctgacga
     5461 cggtaaccgg gcatgccaac catctggtgc atgcggcact ggctggcgtt gagcagatcg
     5521 ttaccgacag ttcagcgtcg cggcagttac gcctcgtgca gtggcgggaa acgcaacctc
     5581 cgtttgacgc cgcggcggca aaaaccattc tctcagatac ccatgacgca gaactgccaa
     5641 tttaccggct ggctgccgac gatccggatg aggagaacac cctcgccacg gcagttttca
     5701 ccctcgatgc caaccacgtc aggtggcaaa ttttcgacat taaccgcgac gatgctaaat
     5761 ttcatggaga agtgcgtggg tgaaatagcc ggtcgtcagt cataaagggc aggggagtac
     5821 cgttggcccg gcgttcgata gtaccgtccg gatactgcca gatatcggcg tccagggaga
     5881 tgtcctgaag cgcggtgtcc ggaatttcag gtttgtgtct ctacaaagac taactatcag
     5941 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     6001 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaatt caccgaggca gttccatagg
     6061 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     6121 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     6181 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     6241 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     6301 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tactaacagg aatcgaatgc
     6361 aaccggcgca ggaacgctgc cagcgcatca acaatatttt cacctgaatc aggatattct
     6421 tctaatacct ggaatgctgt tttcccgggg agcgcagtgg tgagtaacca tgcatcatca
     6481 ggagtacgga taaaatgctt gatggtcgga agaggcataa atgccgtcag ccagtttagt
     6541 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     6601 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     6661 tcgcgagccc atttataccc atataaatca gcatccaggt tggaatttaa tcgcggcctc
     6721 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     6781 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     6841 ttttgagaca caacgtggat ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     6901 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
     6961 aactggccca cctacaataa aggggtcgtt tgcgggagag ggcgaaatcc tacgctaagg
     7021 ctttggccaa cgatattctc cggt
//
LOCUS       DACAPB010000208         6991 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.209, whole genome shotgun sequence.
ACCESSION   DACAPB010000208 DACAPB010000000
VERSION     DACAPB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.209"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(64..822)
                     /locus_tag="IT904_004088"
     CDS             complement(64..822)
                     /locus_tag="IT904_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP3193790.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="IT904_004089"
     CDS             complement(830..1933)
                     /locus_tag="IT904_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP3193791.1"
                     /translation="MTKVLLSHPPRPASHNSNRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /locus_tag="IT904_004090"
     CDS             complement(1943..3124)
                     /locus_tag="IT904_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP3193792.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="IT904_004091"
     CDS             complement(3192..4217)
                     /locus_tag="IT904_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3193793.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4648..4869)
                     /locus_tag="IT904_004092"
     CDS             complement(4648..4869)
                     /locus_tag="IT904_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193794.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5122..6906)
                     /gene="acrF"
                     /locus_tag="IT904_004093"
                     /pseudo
     CDS             complement(5122..6906)
                     /gene="acrF"
                     /locus_tag="IT904_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(6924..>6991)
                     /locus_tag="IT904_004094"
     CDS             complement(6924..>6991)
                     /locus_tag="IT904_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP3193795.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1946 a         1808 c         1625 g         1612 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaactcatca ggtgcagctt gttccactat ctccccacga tccataaaaa tcacccggtc
      181 agcgacggtt cgtgcaaacc ccatctcatg tgttacacac agcattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tcaggatcga gcgccgacgt
      301 tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcgagg taatgcaccg ccagatcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct accacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ctgcccttta ggcagtgcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcaacggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca actgatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agagcaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg tttgagttat ggctcgccgg gcgcggggga tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tgattcagca cggtgccggc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggtggaat aatcacccgc aatgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtcgcga cgctcgttac
     3181 ctcacaccga cttaacgcac gggcggtgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc aaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat caacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     3601 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccagt aaatgccatt cccatcccgg cgtcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacttcc cctgactgta aagccgtgaa gcgttctttt
     3961 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaatat caacatcaat acctgaaaat ttaccgtcag catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgtacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4321 cgcacgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc
     4381 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtgtttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgagaagtg tgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag
     4561 aattagccag cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca
     4621 gagcgcgaaa cttcgcgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gctgctgctc cgccagcaat agctccagcc gtttcccagt tatcctgagt
     4741 tgtgcctttc atacaggtat tggtgtgaac cagacgcccc tgatcgtcat aaaccggaac
     4801 gcagggcgaa tcgtgagcac agcccgccag caatgctgtg agcaatgcca caggaattaa
     4861 tcttttcatg gtgttacctc agtaataaac gctaacgttt aataagttaa ctctcttatt
     4921 aacgagctga atgccatttt atcaccggag gttataaatc ttgcggaatg aataatgaca
     4981 aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagct tttaaagagt
     5041 gagcaaaaga atatcttatt aatttttatg ggtttcactg aaaataacaa aggcacccga
     5101 aagcgccttt atgtttcaga tttatccttt aaagcaacgg cggatcacca caaagaacac
     5161 cggtacgaag aagattgcca gcaacgttgc agagaccatt cctcccatta ccccgatacc
     5221 cactgcgttc tgcgcgccac tgccggcacc gttactgata gctagcggta atacgccgag
     5281 aataaaggcg agagaggtca tcaggatagg acgcagacgc atacgtactg ccatcagtgt
     5341 cgcttcaaca acacctttac cctctttctc catgagatct ttagcgaact caacaatcaa
     5401 aatagcgttt ttggccgaca agccaattgt cgttagcaag cccaccataa agtagacgtc
     5461 atttttttga ttaaagagtg tcgccgccag cagcacgccg acaatcccta acggcacgac
     5521 caacataacc gagacaggaa ttgaccagct ttcatagagt gcagcaaggc acaggaaaac
     5581 aaccacaaag gaaattgcta ccagagcggg agcctggttt cccgataagc gttcctgata
     5641 cgacataccc gtccagtcat aaccaatgcc cgcaggtaat tttgacgcaa ggttttccat
     5701 caacgccatg gcatcgccgg aactggttcc tggcgcggct tccccctgaa tctccattga
     5761 cggcagaccg ttgtagcgtt ccagtcgcgg agagccatac acccaatgtg aagtggtaaa
     5821 ggcagagaat ggcaccattt cgccgttggc gctgcggaca taaagtttat cgacatcttc
     5881 tggcagcata cggaatttgg catccgcctg aacatacacc tttttcacac gaccacggtc
     5941 gatgaagtcg ttaacgtaag tcccacccag cgccgttgaa atggtctgat tgatgtcaga
     6001 aagtgaaaca cctaatgcct gcgccttttc ctggtcaact tccagtttaa actgcgcggt
     6061 gtcttccagg ccattaggac gcacgctgac taagctggca ggatgttgcg cagccatacc
     6121 aagcaactgg ttacgggcct gggttagggc atcgtgaccc agcccagcct gatcaattaa
     6181 ctcaaagtcg aaacccgttg ccgtgcccag ttcaacaatg gctggcatat tgaatggaat
     6241 gacaaaaccg tcgcggatct tgcccaattc cattttggca cgatggatta ccgcttccgc
     6301 actgttttca tcaccattac gctcttccca cggtttcaga ctgacgaagg ccataccggc
     6361 gttttgtgcc tggccgctga agctaaagcc gttaaccgta aagacacttt caacgttcgc
     6421 tttctcgttt ttcagatagt aatccgtcac ttgatccaac actttttgcg tccgctcttg
     6481 cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct cttcaggtaa
     6541 gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca gcgcatagat
     6601 cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt agtggttaac
     6661 gctatgatcg aaggtggtat taaaccagcc gaagaaaccg cccttatttt cgtgatgctc
     6721 agcagagacg ggtttaagca gcgttgcaca taacgcaggg gtaagaatca atgccaccag
     6781 aacagaaagc gccattgccg aaacgatggt gatagagaac tggcgataaa ttgccccagt
     6841 agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg cgatacccac
     6901 cagtgcgcgg taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg
     6961 actttgtcat gcagctccac cgattttgag a
//
LOCUS       DACAPB010000209         6980 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.210, whole genome shotgun sequence.
ACCESSION   DACAPB010000209 DACAPB010000000
VERSION     DACAPB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.210"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(154..855)
                     /locus_tag="IT904_004095"
     CDS             complement(154..855)
                     /locus_tag="IT904_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3193796.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(973..1485)
                     /locus_tag="IT904_004096"
     CDS             complement(973..1485)
                     /locus_tag="IT904_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3193797.1"
                     /translation="MITMKKSVLTAFITVVCVTSSVMAADDNAITDGSVTFNGKVIAP
                     ACTLVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGT
                     ADTTQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDY
                     IATGKATQVM"
     gene            1832..2407
                     /gene="ssuE"
                     /locus_tag="IT904_004097"
     CDS             1832..2407
                     /gene="ssuE"
                     /locus_tag="IT904_004097"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP3193798.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            2400..3359
                     /locus_tag="IT904_004098"
     CDS             2400..3359
                     /locus_tag="IT904_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP3193799.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLYQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            3356..4501
                     /gene="ssuD"
                     /locus_tag="IT904_004099"
     CDS             3356..4501
                     /gene="ssuD"
                     /locus_tag="IT904_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAP3193800.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLQRETVDFNGKHIHVRGA
                     KLLFPAIQQPYPPLYFGGSSDVALELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPLLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            4512..5303
                     /gene="ssuC"
                     /locus_tag="IT904_004100"
     CDS             4512..5303
                     /gene="ssuC"
                     /locus_tag="IT904_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAP3193801.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            5300..6067
                     /gene="ssuB"
                     /locus_tag="IT904_004101"
     CDS             5300..6067
                     /gene="ssuB"
                     /locus_tag="IT904_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP3193802.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6110..>6980)
                     /gene="pepN"
                     /locus_tag="IT904_004102"
     CDS             complement(6110..>6980)
                     /gene="pepN"
                     /locus_tag="IT904_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAP3193803.1"
                     /translation="RHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNEM
                     AELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRNA
                     CLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWHQ
                     NGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFHA
                     KDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLENL
                     SGDLYEKITKALA"
BASE COUNT         1649 a         1754 c         1822 g         1755 t
ORIGIN      
        1 aaaggcagga tcgaaccagg tttcagcttg tgcattgaac gctgccgaca caaacaccac
       61 cagcccacca ataaacgatg gcactccacc agagcttaac aggctgtgtt gtcggtgagt
      121 tctatacatc tcgctctctt aactgaagac gaattacgat ttacagttga gttgcggcgt
      181 ttccgccccg tagtcattga tggttcgata cgttacgctg ctatttttgg caccctttgg
      241 cagagagaat tggtatttat caaaggggga aatcatttga tttttaacta cctttccacc
      301 tattttcagg tctgtcaggg taatgaaata aggtgttggg ttactgaccg taaccatgtc
      361 agctgacgaa gtgcaacgca gtttcgccgg agcctcggct aagggatacg ctaaaccttt
      421 tggtcgccag aaaagtttga tgacgctttg tgtggcaatc ttcagggtat ttccctcttc
      481 ccgcttcgtt gttgaaggga ctgcccgtac gctagtgaag aaaagggttt ctctgtcttt
      541 cgccaacggc gctccaatgt acataatgcg taacagattc tcatttgccg gattcagcac
      601 aaatagcggt ggtgtaataa taaagtctgc tgatttttta ttatctgggg tcatcaccca
      661 tgactgaata agataaggaa catccggact ggtgttacgt acacccagtg aaatttgctc
      721 ttcaccttct gaataaacaa gacgtgttgc tgcaagtcca atcccgcctt tacctgccgc
      781 ccatgcggat gagcagctga gtaatattgc cgttaaactt acggtcacaa ttccctttgt
      841 tatgcaagtt ttcataaata gtcactctta aaaaatatga atatcccctc cagaacactg
      901 gaggggtact aattaataaa acaattaatg agttctatta ataatagtta atatggaaat
      961 ttgttaccgc attcacatta cctgagtcgc tttccccgta gcgatataat caactttaaa
     1021 agtcaacgtc tgatctccat cctgcaacaa aatgttcccg gtttctgtgt ctggcgtgat
     1081 ggccgttgta ccatcattca tatacatttg cagggccacg tttgtagcag catcagatga
     1141 ggcctggtta gcaaacgctg taatctgagt agtatccgca gtgccattaa aggtgaacgt
     1201 tgcatttttt gttacggtag tatcacaatc ttttaattca attggcacat cagtttgcac
     1261 gccagaaacc tgaccattgg tttgcaactt cgtggcacta acatctggca aagtcaccac
     1321 ggaatctttc gtcgcagcta ccagggtaca agctggagca ataactttac cattaaatgt
     1381 tactgagcca tccgtgatag cattatcatc agcagccata acgctggacg ttacacatac
     1441 cacagttata aacgccgtca atacactttt tttcatcgtt atcatcctga tctcttcgta
     1501 aatatgataa tgcctttaaa tgacattaga cttaataata acaataattg tccgccattt
     1561 aaatcaagca tactaaatcc aataagtgac aaatctttaa tttatagatt tcatgaatat
     1621 ttaaatatta actgaacgag taattcattg attcaacatc taatgactat ccaacaaata
     1681 aatatgcgtg atatataaag ccctttcttt agtttatttt cagaaaaaga tacacaaaat
     1741 ttatatttgg cattttcttg tctctccgac agactgaccg aattgttatc aatgttaaca
     1801 aaaaaagaac aattggttat aaggagagag tatgcgtgtc atcaccctgg cgggtagtcc
     1861 tcgctttcct tctcgctcca gctccttgct ggaatatgcg cgggaaaaac taaatggcct
     1921 ggatgtagag gtttatcact ggaatctgca aaacttcgcc ccggaagatc tgctttatgc
     1981 tcgtttcgat agtccggcac tcaagacctt caccgaacag ctgcaacagg ctgatggact
     2041 gatagtcgct acacctgtgt ataaagccgc ctattccggc gcgctgaaaa ccctgctcga
     2101 cctgctgcca gagcgcgctt tgcaaggcaa agtggtgcta ccgctggcga cgggcggtac
     2161 cgtggcccat ctgctggcgg tcgattatgc ccttaaacca gttttaagcg cactgaaagc
     2221 tcaggagatc ctgcacggtg tgtttgccga tgactcacaa gtaattgatt accatcacag
     2281 accccagttc acgccaaatc tgcaaacccg tcttgatacc gcgctagaaa ctttctggca
     2341 ggcattgcac cgccgcgatg ttcaggttcc tgaccttctg tctctgcgag gtaatgccca
     2401 tgcgtaacat cattaaactg gcgctggtgg gattgcttag cgtctctacg attgcggttg
     2461 ctgcagaatc ctcgcctgaa gcgttacgta taggctatca gaaaggcagt attggtatgg
     2521 tactggcaaa aagccaccag ttactggaaa aacgctatcc gcaatcaaaa atctcctggg
     2581 tggagttccc cgcgggtccg caaatgttgg aagcgttaaa cgttggcagt attgatctcg
     2641 gcagtaccgg ggatattccg ccaatctttg cccaggctgc cggggctgat ttggtatatg
     2701 tgggcgtcga gccaccgaag cccaaagccg aagtgattct ggtggcagaa aacagcccga
     2761 tcaaaaccgt agccgatctt aaaggtcaca aagttgcctt tcagaaaggt tccagttcac
     2821 acaacctttt actgcgtgca ctgcgtcagg ccggacttaa gtttaccgat atccaaccca
     2881 cttacctgac gcccgctgat gcccgcgccg cgttccagca aggtaacgtt gacgcctggg
     2941 ctatctggga tccctactac tctgctgcat tattacaggg cggcgtgcgg gtgctgaaag
     3001 acggcaccga tctctatcaa actggatcgt tttatctggc agctcgtccc tatgcagaaa
     3061 aaaacggcgc ttttattcag ggcgtactgg caacctttag tgaggccgat gcgttaaccc
     3121 gcagccagcg cgagcaaagc atcgctttac tggcaaaaac gatgggctta ccggcaccgg
     3181 tgattgcctc ttacttagat catcgccctc ctaccaccat caaaccggtt aacgccgagg
     3241 ttgccgcctt acagcagcaa acggcagatc tgttttatga aaatcgtctg gtgccgaaaa
     3301 aagtcgatat tcgccagcgc atctggcagc ccactcaact ggaaggaaaa caattatgag
     3361 tctgaatatg ttctggtttt taccgaccca cggtgacggg cattatctgg gaacggaaga
     3421 aggttcacgc ccggttgatc acggttatct gcaacaaatt gcgcaagcgg cggatcgtct
     3481 tggctatacc ggtgtgctaa ttccaacggg gcgctcctgc gaagatgcgt ggctggttgc
     3541 cgcatcgatg atcccggtga cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt
     3601 aacctcacct accgttgccg cccgccaggc cgccacgctt gaccgtctct caaatggacg
     3661 tgcgttgttt aacctggtca caggcagcga tccacaagag ctggcaggcg acggagtgtt
     3721 ccttgatcat agcgagcgct acgaagcctc ggcggaattt acccaggtct ggcggcgttt
     3781 attgcagaga gaaaccgtcg atttcaacgg taaacatatt catgtgcgcg gagcaaaact
     3841 gctcttcccg gcgattcaac agccgtatcc gccactttac tttggcggat cgtcagatgt
     3901 cgccctggag ctggcggcag aacaggttga tctctacctc acctggggcg aaccgccgga
     3961 actggttaaa gagaaaatcg aacaagtgcg ggcgaaagct gccgcgcatg gacgcaaaat
     4021 tcgtttcggt attcgtctgc atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc
     4081 cgccgagcgg ttaatctcgc atcttgatga tgaaactatc gccaaagcac aggccgcatt
     4141 cgcccggacg gattccgtag ggcaacagcg aatggcggcg ttacataacg gcaagcgcga
     4201 caatctggag atcagcccca atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg
     4261 gacggcgctg gtgggcgatg gtcctacggt cgctgcgcga atcaacgaat atgccgcgct
     4321 tggcatcgac agttttgtgc tttcgggcta tccgcatctg gaagaagcgt atcgggttgg
     4381 cgagttgctg ttcccgcttc tggatgtcgc catcccggaa attccccagc cgcagccgct
     4441 gaatccgcaa ggcgaagcgg tggcgaatga ttttatcccc cgtaaagtcg cgcaaagcta
     4501 aggaagaaat aatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt
     4561 taccggtggg catcgtggcg gtgtggcaac tggcctcctc ggttggctgg ctgtcgacgc
     4621 gtattttgcc ttcaccggaa ggagtggtga cggcgttctg gacgctctcc gccagcggcg
     4681 aactgtggca gcatctggcg atcagctcct ggcgggcgct gattggcttt tcaattggcg
     4741 gatcgctggg gttgattttg ggactgatta gcgggctatc tcgctgggga gaacggctgc
     4801 tggatacctc aattcagatg ttgcgcaacg tgccgcatct ggcgctgatc ccgctggtga
     4861 ttttgtggtt tggcattgat gaatccgcaa aaatctttct ggtggcgctc ggtacgctat
     4921 tccccattta tatcaacacc tggcatggca tccgtaatat cgatcgcggg ttggtggaga
     4981 tggcgcgtag ctatggatta tccggcatac cgctgtttat ccatgtgatc ctgcctggtg
     5041 ccctgccctc aattatggtc ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga
     5101 ttgttgccga aaccatttct gccaattcag gcattggtta tctggcgatg aatgcgcggg
     5161 agtttttgca aacggacgtg gtggtagtcg ctattattct ttacgccctg ctcggcaaac
     5221 tggctgacgt cagcgcgcag ctgttagagc gcctctggtt gcgctggaac ccggcttatc
     5281 atttgaagga ggccacggta tgaatactgc tcgtctgaac cagggcacgc cattgttgct
     5341 caatgcagta agcaaacatt acgcggaaaa tatcgtcctg aaccaactgg atttacatat
     5401 tccggcaggt cagtttgtgg cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct
     5461 gcgcctgctg gcaggtctgg aaacgccaac cgcaggcgat gtgttagcgg gcaccacacc
     5521 gctggctgaa attcaggaag acacgcgaat gatgtttcag gatgcgcgtc tgctaccgtg
     5581 gaaatcggtg attgataacg ttgggttagg ccttaaaggc cagtggcgcg atgccgcacg
     5641 tcgagcgttg gctgcggtag gactggagaa tcgcgcaggg gaatggcctg ccgcactttc
     5701 tggcgggcag aaacagcgag tggcgctggc aagagcgttg attcatcgac cgggattatt
     5761 gttgcttgat gaaccgctcg gggcgctgga cgccttaacg cgactcgaga tgcaggattt
     5821 gattgtgtca ctttggcagg agcacggctt taccgtactg ttggtgacgc atgatgtgag
     5881 cgaagcggta gcgatggctg accgggtgct gttaattgaa gagggaaaaa ttggtctgga
     5941 tttgacagtg gatatccccc gtccgcgtcg attagggtcg gtgcgattag cggagctgga
     6001 agcggaggtg ttgcaacggg tgatgcaacg tggtgaatct gaaactcgcc tgagaaaaca
     6061 ggggtaaatt ccccgaatgg cggcgctatt gccgccattc ggttatttat caagccagtg
     6121 ctttagttat cttctcgtac agatcgccag agagattttc cagccctttc aactgttcca
     6181 gcgccgcgcg cattttctcc tgacgtttgg catcgtaacg tttcaggcga atcagcggtt
     6241 caatcagacg tgaagccacc tgcgggttac ggctgttgag gtcggtaagc atttccacca
     6301 ggaacaggta accgctgcca tctttggcat ggaacgctgc cggattgctg cccgcaaacg
     6361 cgccaatcaa cgaacgaata cggttcgggt tgctcatggt aaatgagcga tgctgcaaca
     6421 ggccgcgcac cgtctccagc acattcgccg ccgggctggt ggcttgcagg ataaaccatt
     6481 tatccatcac cagaccgttc tgatgccact tgtcgtcgta ctcctgcatc agcgcgtcac
     6541 ggcaaggcag ctgtgcggca accgccgcag aaagcgccgc cagcgcatca gtcatattgt
     6601 ttgcttcgtg gaactgcttg ctcaccagca catcagccag atgcgtttca ccaaaagcga
     6661 ggaaacgcag gcaggcatta cgcagagtgc gttttgcaat atcttcatgc tcaacacggt
     6721 actcgctctg gtaattcgcg ttgtaaatag ccagcagctc atccgccagt tcagtcgcca
     6781 gagtacgagt gagtgcttcg cgtacttcgg caatagcaat cgggtcgatg atatcgaaca
     6841 attcagccat ttcattgaca gaaggcagcg tcaggatttc tgccgccagc gctggatcaa
     6901 tcttctcatc aagcagtacc gcgcggaaag catcagccac atgcaccggc agagacagcg
     6961 gctgaccttg ctgatgccgc
//
LOCUS       DACAPB010000210         6913 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.211, whole genome shotgun sequence.
ACCESSION   DACAPB010000210 DACAPB010000000
VERSION     DACAPB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.211"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            76..285
                     /locus_tag="IT904_004103"
     CDS             76..285
                     /locus_tag="IT904_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193804.1"
                     /translation="MSYSYGMFESPVVDPQQGMNAAKARCAAWGYSGAEPFGGFTSQC
                     SQPSSSGCMQTLVTIEYQCTGDIKK"
     gene            complement(319..525)
                     /locus_tag="IT904_004104"
     CDS             complement(319..525)
                     /locus_tag="IT904_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193805.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1023..1096)
                     /locus_tag="IT904_004105"
     tRNA            complement(1023..1096)
                     /locus_tag="IT904_004105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1062..1064),aa:Gly,seq:ccc)
     gene            complement(1175..1930)
                     /locus_tag="IT904_004106"
     CDS             complement(1175..1930)
                     /locus_tag="IT904_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP3193806.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2345..4642
                     /gene="xdhA"
                     /locus_tag="IT904_004107"
     CDS             2345..4642
                     /gene="xdhA"
                     /locus_tag="IT904_004107"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAP3193807.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNAQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYDYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQKGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNIHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4653..5531
                     /gene="xdhB"
                     /locus_tag="IT904_004108"
     CDS             4653..5531
                     /gene="xdhB"
                     /locus_tag="IT904_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAP3193808.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQPKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5528..6007
                     /gene="xdhC"
                     /locus_tag="IT904_004109"
     CDS             5528..6007
                     /gene="xdhC"
                     /locus_tag="IT904_004109"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAP3193809.1"
                     /translation="MNHSETITIECTIDGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6047..>6913)
                     /locus_tag="IT904_004110"
     CDS             complement(6047..>6913)
                     /locus_tag="IT904_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP3193810.1"
                     /translation="GKEVVARAIHKLSGRRNKPFIAINCAAIPEQLLESELFGYVKGA
                     FTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREILPIGASSPIQVD
                     IRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTR
                     RLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNN
                     GTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSKKQVADELGIGIATL
                     YRKIKKYELLNT"
BASE COUNT         1732 a         1764 c         1684 g         1733 t
ORIGIN      
        1 ttgattattt ctggatgtgc aacaaaaaaa caactcgttc caactggtgg aagcaaatca
       61 gacggcacag ttaggatgag ttattcttac ggtatgtttg aaagtcctgt tgtagatcca
      121 caacaaggca tgaatgcagc caaagcgcgt tgtgccgctt ggggatattc aggagccgag
      181 ccttttggtg gattcacttc tcaatgttca caaccttctt catctggttg tatgcaaaca
      241 cttgtgacta tcgaatatca atgcactggc gatattaaga agtaataatt aggggcttcc
      301 agccccttct ataaccattc atggaacagg gacaaccgaa atcactttaa accctgaaac
      361 agccgttact ccattagatg gagaattaaa tctcacagta tcttttctta ctgcatcatg
      421 aatttcatct tttgttggtt catgctctga atggaataaa acctcttcca ttctaatttg
      481 atcaccgtct ctatgctcat aagtaatctt gtagctaaca gtcatatcaa cctcatgcag
      541 gggaattgtg agattattat agacaataga aaccataaag ccccgttggt gatacagcaa
      601 caagcgggag cgtagcgact tcatcagatt gtcagaaaga tcacacctta tcaggataac
      661 cgtgtacccg ttgggagcgt ggctgaaacg ctgatagagc ttgcatatgg tttgcaagga
      721 ccaatatcac cataaacctg tttacgcagc cacaaggccg cacatcgggc ttaaatgcgt
      781 gtgttcagtc tgtcgatgac attacaatgc caggatgggc tttatcttca cgttgtgcta
      841 tcgcggtgtg agaggtgcaa ctgccgccaa cgttaggtta tactgtcatc tcaccgtgta
      901 acttaacgtg acagttacta agagcggatc acaatggtag aactcctaag caaattaaat
      961 aggtaactgt ttaagttgct gacagagaag acttttttaa tcttctccac ctggggagat
     1021 tctggagcgg gcgaagggaa tcgaaccctc gtatagagct tgggaagctc tcgttctacc
     1081 attgaactac gcccgcttcg agatgcgtaa ggcattataa accttacgct ctccttagca
     1141 agtgccgcgc tgctgactgc tgattaattc gccatcagca ttttggcttg ctgccctgcg
     1201 gcggcaagta acgtagcgga tcaattgccg ttgcacggta acgaatctgg aaatgcaggc
     1261 gaacagatgc cgcatccgtg ctccccatag tggcgatttt ttgcccagcc ttcacgcttt
     1321 gcccattatt taccagcatc gtgtcattat gggcgtaagc cgtaatgtaa tcttcactgt
     1381 gtttaatcat gatgagatta ccgtagccac gcagctggtt gcccacatac accacctttc
     1441 ctgcacccgc ggcgtaaata ggtgtacccc gtggagctga gatatcaatc cctttattgc
     1501 cgccatctgc tgtcgaatac ggcatgataa ctttccctgt cgttggccat aaccaacaac
     1561 gttgccctac tggcggccag gaagatttcg gtaccgctga tgacggtgta accgatgcgg
     1621 ttttggtcgt tgatttggcg gttgatttac gtgtactact gctacttttc gccccaccca
     1681 gttttagttt ctgaccaact tcaatggtgt aaggggggga aatgccgttc agtcgcgcca
     1741 gttcttttac gctggttccc gtggtgcgcg aaatacgata tagcgtatcc ccccgtttca
     1801 cggtgtaaac ggagccggaa tacgttcctg tatcggatga tttgctaccc gaacagcccg
     1861 ccaaaagcag tccaaccgat aacaacatca cgatacccag agatttttta ttcaggcgtc
     1921 ccgcactcaa aatctttcct cgcttaaaaa acaagacgcc ttatcatagc agccagcctc
     1981 ttgataccca atctcttgct tctggaaaaa cgtggtaata gagaaagagg ttaatttcat
     2041 ttggcaaagg gaagtatcat cagcaaaata ttcgctgtag aggtatcgaa cttcctgact
     2101 ttttacccta tttcccatcc tgatgcgtaa taatcccaat ttatcacatt gattttatcg
     2161 ccactcatat tgatacgtat cactgcccct attcagagtc ctgtttcgta tcatatctgt
     2221 gtttttttaa cgtcatcaca cttcctacct cccctaacgc ttatcgtcgt ttctggcgta
     2281 aatcttgcct gcttagacta aatctttgcc ataagaacca aatgttcaaa tcgaggggat
     2341 ttctatggaa gcgcgggaag caaccgctac gggtgaatca tgcatgcgcg tcgatgccat
     2401 tgctaaggtc accgggcggg cacgatatac tgacgattat gttatggcgg gcatgtgtta
     2461 tgcgaaatat gtacgtagcc ctatcgcaca tggttatgcc gtaagtatta atgatgaaca
     2521 agccagaagt ttgccgggcg tactggcgat ttttacctgg gaagatgtgc ctgatattcc
     2581 attcgctaca gctgggcatg cctggacact tgacgaaaac aagcgcgata ccgccgatcg
     2641 cgcactgcta actcgccatg ttcgtcatca tggtgacgcc gttgccatcg tcgtggcccg
     2701 cgatgaactc acggcagaaa aagcggcgca attggtcagc attgagtggc aagaattacc
     2761 cgttatcacc acgccagaag cggcgctggc agaggacgct gcaccaatcc ataacggcgg
     2821 caatttactg aaacaaagca cgatgtcgac gggcaatgcc caacaaacaa tcgatgccgc
     2881 cgactaccag gtacaggggc actatcagac ccccgttatt caacattgtc acatggaaag
     2941 cgtaacatcg ctggcgtgga tggaggatga ctcgcgaatt accatcgttt ccagcaccca
     3001 gatcccgcac attgttcgcc gcgtggttgg tcaggcgctg gatattccct ggtcatgcgt
     3061 acgagtcatc aaaccatttg tcggtggcgg ttttggtaat aaacaggatg tactggaaga
     3121 gccaatggcg gcattcctga ccagcaagct tggcggcatt ccggtgaaag tttcccttag
     3181 ccgtgaagag tgtttcctcg caacccgtac ccgccacgct tttactattg acgggcaaat
     3241 gggcgtgaac cgcgacggaa cattgaaagg ttatagtctg gatgttctgt ctaacaccgg
     3301 cgcttatgca tctcacgggc actccatcgc ttctgcgggg gggaataaag tcgcttacct
     3361 ttatcctcgt tgtgcctacg attacagttc aaagacctgc tataccaacc tcccctcggc
     3421 tggtgcgatg cgtggttatg gcgcgccaca agtcgtattt gccgttgagt ctatgcttga
     3481 tgatgccgcg acagcgttag gtattgatcc tgttgaaatt cgtttacgca acgccgcccg
     3541 cgaaggagat gctaatccgc tcacgggcaa acgtatttac agcgcagggt tgccggagtg
     3601 tcttgaaaaa ggccggaaaa tctttgaatg ggaaaaacgc cgtgcagagt gccagaacca
     3661 gaaaggcaat ttgcgccgcg gcgttggcgt cgcctgtttt agctacacct ctaacacctg
     3721 gcctgtcggc gtagaaatag caggcgcgcg ccttctgatg aatcaggatg gaaccatcaa
     3781 cgtgcaaagc ggcgcgacgg aaatcggtca gggtgccgac accgtcttct cgcaaatggt
     3841 ggcagaaacc gtgggggttc cggtcagcga cgttcgcgtt atttcaacac aagataccga
     3901 cgttacgccg ttcgatcccg gcgcatttgc ctcacgccag agctatgttg ccgcgcctgc
     3961 gctgcgcagt gcggcactat tattaaaaga gaaaatcatc gctcacgccg cagtcatgct
     4021 acatcagtca gcgatgaatt tgaccctgat aaaaggccat atcgtgctgg ttgaacgacc
     4081 ggaagagccg ttaatgtcgt taaaagattt ggcgatggac gctttctacc accctgaacg
     4141 cggcgggcag ctctctgctg aaagctccat caaaaccacc actaacccac cggcgtttgg
     4201 ctgtaccttt gttgatctga cggtcgatat tgcgctgtgc aaagtcacca tcaaccgcat
     4261 cctcaacatt catgattcag ggcatattct taatccactg ctggcagaag gtcaggtaca
     4321 cggcggaatg ggaatgggta ttggctgggc gctatttgaa gagatgatca tcgatgcgaa
     4381 aagcggcgtg gtccgtaacc ccaatctgct ggattacaaa atgccgacca tgccggatct
     4441 gccacaactg gaaagcgcgt tcgtcgaaat caatgagccg caatccgcat acggacataa
     4501 gtcactgggt gagccaccaa taattcctgt tgccgctgct attcgtaacg cggtgaagat
     4561 ggctaccggt gttgcaatca atacactgcc gctgacgcca aaacggttat atgaagagtt
     4621 ccatctggca ggattgattt gaggataaca tcatgtttga ttttgcttct taccatcgcg
     4681 cagcaaccct tgccgatgcc atcaacctgc tggctgacaa cccgcagccc aaactgctcg
     4741 ccggtggcac tgacgtactg attcagctcc accatcacaa tgaccgttat cgccatattg
     4801 ttgatattca taatctggcg gagctgcggg gaattacgct ggcggaagat ggctcgctac
     4861 gtatcggctc tgcaacgaca tttacccagc taatagaaga tcctataact caacgtcatc
     4921 tcccggcgtt atgtgctgcg gcctcgtcca ttgctggacc gcagatccgt aacgtcgcta
     4981 cctacggtgg aaatatttgc aacggtgcca ccagcgcaga ttctgccacg ccaacgctaa
     5041 tttatgacgc gaaactggag atccactccc cgcgcggtgt tcgtttcgtc ccgattaatg
     5101 gctttcacac cgggccgggc aaagtgtctc ttgagcatga cgaaatcctt gtcgcctttc
     5161 attttccgcc ccagccgaaa gaacacgtgg gcagcgcgca ttttaaatat gccatgcgcg
     5221 acgcaatgga tatttcaacg attggctgcg ccgcacattg ccgactggat aacggcaatt
     5281 tcagcgaatt acgcctggca tttggtgttg ccgcgccaac gccgattcgc tgccaacatg
     5341 ccgaacagac tgcacaaaat gcgccattaa acctgcaaac gctggaagct atcagcgaat
     5401 ctgtcctgca agatgtcgcc ccgcgttctt catggcgggc cagtaaagag tttcgtctgc
     5461 atctcatcca gacgatgacc aaaaaagtga ttagcgaagc cgtcgccgcg gcggggggaa
     5521 aattgcaatg aatcacagcg aaacaattac catcgaatgc accattgacg ggatgccttt
     5581 tcagcttcac gccgcaccag gcacgccgct ctcggaatta ctccgcgaac aaggactgct
     5641 aagtgtcaaa caagggtgct gcgtgggtga atgtggtgcc tgtacggtgc tggtcgacgg
     5701 cacagcaata gacagttgct tataccttgc cgcctgggct gaaggaaaag agatccgcac
     5761 gctggaaggt gaagcgaaag gcggaaaact ttctcatgtt cagctggctt atgcgaaatc
     5821 cggcgcagtg cagtgcgggt tttgtactcc tggtctgatt atggctacca cagcaatgct
     5881 ggcgaaacca cgcgagaagc cattaaccat tacggaaatt cgtcgcggac tggcgggaaa
     5941 tctttgtcgc tgcacggggt atcagatgat tgtaaatacg gttctggatt gcgagaaaac
     6001 gaagtaaaag gatatccggc ctgaattcag gccggattca ctgaggttat gtgtttaaca
     6061 actcatattt cttaatcttg cgatagagcg tagcaatgcc gatgcccagt tcatcagcaa
     6121 cttgcttctt gctgttatga cgtgaaagcg cctcgcggat catttgcttt tccatctcct
     6181 ccagcgccgt gccgcccgca tcatcgagtg acaggtgtgc ctcagtgacc tctgttacat
     6241 cactttgctc cgttgtgcca ttattcagca gatttggcgg caatagcgtg ctgtcgataa
     6301 cttcacctga aggaacaacg ttaaccagat attccatcaa attgcttaac tcgcgcaggt
     6361 ttccgggcca acgatgctta cgcaatattt cgacgacatc gggagcaatg ccaggataaa
     6421 ccgatcccag acgacgggta tgcagatgta aaaagtaatg caccagtagt tcaatatctt
     6481 cctgacgttc acgcagcggt ggcagagtta tcgggataac attaagtcgg tagaagagat
     6541 cttcgcggaa tttaccttcg gcaatgaact gggccaaatt ctggttagtt gcagaaatga
     6601 tgcgaatgtc gacttgtatt gggctactgg caccaatcgg tagaatttca cgtgcctcaa
     6661 tagcgcgcag taatttagcc tgcaacatta atggcatatc acctatttca tcgagaaaca
     6721 gcgtgcccgt attcgccgcc tgaatcaacc ctgttttacc gttggcagaa gcgccagtaa
     6781 atgcaccttt aacataaccg aacagttcgc tctccagaag ctgctccgga atcgcggcac
     6841 agttgatagc aataaagggt ttattccgtc ttccgctcaa cttatggatt gcacgggcga
     6901 cgacttcttt acc
//
LOCUS       DACAPB010000211         6903 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.212, whole genome shotgun sequence.
ACCESSION   DACAPB010000211 DACAPB010000000
VERSION     DACAPB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.212"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(59..2488)
                     /locus_tag="IT904_004111"
     CDS             complement(59..2488)
                     /locus_tag="IT904_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP3193811.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(2513..3613)
                     /locus_tag="IT904_004112"
     CDS             complement(2513..3613)
                     /locus_tag="IT904_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAP3193812.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQTATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(4001..4747)
                     /gene="cutC"
                     /locus_tag="IT904_004113"
     CDS             complement(4001..4747)
                     /gene="cutC"
                     /locus_tag="IT904_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP3193813.1"
                     /translation="MALLEICCYSMECALTVQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(4761..5327)
                     /locus_tag="IT904_004114"
     CDS             complement(4761..5327)
                     /locus_tag="IT904_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP3193814.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            5543..>6903
                     /gene="argS"
                     /locus_tag="IT904_004115"
     CDS             5543..>6903
                     /gene="argS"
                     /locus_tag="IT904_004115"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP3193815.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEG"
BASE COUNT         1668 a         1839 c         1624 g         1772 t
ORIGIN      
        1 atcaggcagc tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt
       61 atgcctgaat agctggctga tcaaacgccg ttaacttcgg cgcattgccc gtatattttt
      121 cgatatacac cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat
      181 ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc attttccaga tctggccatg
      241 caccttcatg cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca
      301 gaatctgacc acgtttgttc cacacacgca ccagatcacc attcgcaata ccaaagcgag
      361 cagcatcttc ggtgtgaata gtgattggtt cacgatctgc aaccgcatat tttttacgca
      421 gttccgcata gttaagctga ctatgtaaac ggtgtgccgg atgtgcggtc agaagctgca
      481 actgcttctc gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg
      541 ggcaatcctt atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg
      601 gcgtacccag cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt
      661 cgttcttctc gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca
      721 tcggcatagt cacgcgttgc gcacgggcac ctttctgagc ggcatcatag aaaaatttca
      781 gccacgccat ttcatcttta ccttcggtat agatctcttt tccgccaggt ttcagtaatt
      841 ccgcaagatc ggcaaacaca tcaaaatcgt ttcgcgcttc aaattgcgga gcgacagcct
      901 gcttcatcgg cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct
      961 caaacgatgt ggtgatcggt aatacgatat ctgcatgttt agcagctgcg gtccagtagc
     1021 attcagaaac gacgatcatc tccggtttct gccatgcttt aatcagacga ttagtatcct
     1081 ggtgatgggt aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt
     1141 gctctttacc gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg
     1201 caacaggaat cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg
     1261 ccgcagacat ttccggcagc acgccgccaa cacgtgtcgg gttgccacca ttggagtaat
     1321 gataagagaa accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg
     1381 tcaccagcat ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca
     1441 tcagcattgt gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta
     1501 cgccagtaat ttccgccgcc caggccgcgc tcttcggagt attatcgctc ttacctgtca
     1561 gatattcttc aaactgcgga tacccggtgg tgtatttttc aaggaacact ttatcgtgtt
     1621 tgccttgtgt catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat
     1681 tcggcgcgat ccaggtggca ttatcgccaa agaactcaat ggtttcggag cggataggat
     1741 caatggcaat cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct
     1801 catcggtact gctccaggca atttttaagg tattcagcgg gttcatcccc cacagcacca
     1861 caacctggct gttttccaga atcagcggcc aggaggtctg ctgttcatac acctctacag
     1921 aaccgaccac atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc
     1981 cggaataacc gcccgcgagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca
     2041 cgccagaaga gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac
     2101 gaatacgatc gtgttgctca tgaatcagtt ttaatgcttg ttcccagctc acctgtacat
     2161 aggtatcttc gcctcgacct ttcgccggtt gcagtggatt atcgagatag ctttttctca
     2221 ccatcggatg ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg
     2281 agttcggtat ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt
     2341 ctacgttcat cgctccccag cgtcccgcgg tgaggatttt accgcccttc tcttctgccc
     2401 atgcgggtag cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt
     2461 taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag gatcactgtt
     2521 tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt
     2581 cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt
     2641 aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca agtttttcag
     2701 cgtattgcca tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag
     2761 acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg
     2821 ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga
     2881 gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac
     2941 tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag
     3001 aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggtc tgactttcca
     3061 gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat
     3121 gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt
     3181 cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt
     3241 cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact
     3301 tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta
     3361 atttagcaaa taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac
     3421 gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca
     3481 tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca
     3541 gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt
     3601 ttttccctcg catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta
     3661 aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata
     3721 aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt
     3781 agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa
     3841 tatttttagc acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg
     3901 ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg
     3961 agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt
     4021 caatgattcc tttcatttca gcaaccgccg ccccgtctac gacataacgc gaatactcgt
     4081 ccgcgtgtgc atctgatgac atagacaatc cttgattacg ataacgcatc ggtgaggctt
     4141 gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt
     4201 tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg gcaataagtt
     4261 ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agcacccggg
     4321 caatgcccaa ttccgcaaga ttattgagtg tatttagagg gttagcgcac atatcaaagg
     4381 cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca
     4441 tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc
     4501 ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac
     4561 caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca
     4621 gtacacccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt
     4681 ctgcgccgtt ttgctgcacc gttagtgcac attccatgct gtaacagcaa atttccagta
     4741 atgccatttt tactccttaa ttacgccgac tgctcgctgg caacgatctc ttcaatggac
     4801 cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat
     4861 cgctcatgct caccttttgg cgaactggtt ttcactgaga tccccggtag gctgagtcct
     4921 tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg
     4981 atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc
     5041 acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc
     5101 ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa cccgcgacgc
     5161 cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga
     5221 gtgatattca accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa
     5281 tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt
     5341 ctttcagtaa aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg
     5401 ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca
     5461 gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc
     5521 ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca
     5581 ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc agtcagcaaa
     5641 agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat
     5701 ggcaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag
     5761 caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc
     5821 tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca
     5881 gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct
     5941 gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg gtcacaaagt
     6001 gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct
     6061 ggaaaagcag cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta
     6121 ccgcgatgcg aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta
     6181 cgtggtaaaa ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga
     6241 catcaccatg acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga
     6301 tgacgtgatg ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa
     6361 agccaaaggt ctggcagtag aaagcgaagg ggcgaccgtt gtattccttg atgagtttaa
     6421 aaacaaggaa ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta
     6481 caccaccact gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt
     6541 gctgtattac atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg
     6601 taaagcaggc tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct
     6661 gggtaaagac ggcaaaccgt tcaaaacccg cgcgggtggt acagtgaaac tggccgatct
     6721 gctggatgaa gccctggaac gtgcacgccg tctggtggca gaaaagaacc cggatatgcc
     6781 agccgacgag ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga
     6841 tctctccaaa aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga
     6901 ggg
//
LOCUS       DACAPB010000212         6901 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.213, whole genome shotgun sequence.
ACCESSION   DACAPB010000212 DACAPB010000000
VERSION     DACAPB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.213"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(125..691)
                     /gene="satP"
                     /locus_tag="IT904_004116"
     CDS             complement(125..691)
                     /gene="satP"
                     /locus_tag="IT904_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAP3193816.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(840..1553)
                     /gene="msyB"
                     /locus_tag="IT904_004117"
     CDS             complement(840..1553)
                     /gene="msyB"
                     /locus_tag="IT904_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAP3193817.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1579..1983)
                     /locus_tag="IT904_004118"
     CDS             complement(1579..1983)
                     /locus_tag="IT904_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAP3193818.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2360..4276
                     /gene="dnaK"
                     /locus_tag="IT904_004119"
     CDS             2360..4276
                     /gene="dnaK"
                     /locus_tag="IT904_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP3193819.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4365..5495
                     /gene="dnaJ"
                     /locus_tag="IT904_004120"
     CDS             4365..5495
                     /gene="dnaJ"
                     /locus_tag="IT904_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP3193820.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDQEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5758..6870)
                     /locus_tag="IT904_004121"
     CDS             complement(5758..6870)
                     /locus_tag="IT904_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HAP3193821.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT         1783 a         1721 c         1801 g         1596 t
ORIGIN      
        1 cagttgctgg aagggccata cgttgaaacg gcaccagaag tggttgctgc attcagaccg
       61 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaaa
      121 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagtactt
      181 cacccatcgc cagatagatt gcgctggcac cgcagatcag cccaatccag ccggcaaagt
      241 ggatgattgc ggcgttaccg gcaatgttac cgatcgccag cagggcaaac agcacggtca
      301 ggctaaagaa aacgaattgc agaacgcgtg cgcctttcag cgtgccgaag aacataaaca
      361 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
      421 tcagacccag tttcggcatc agcagaatcg caaccagcgt cagccagaaa gaaccgtaag
      481 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccagcaaaaa
      541 tttgcgcgat gccgccgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
      601 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
      661 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
      721 ggtgaaataa tttccctgaa taactatagt gttttcaggg cgcggcataa taatcagcca
      781 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
      841 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
      901 gtcacgcgat aggcgctgcc gctgaccgct ttaaccccat ttagtgccgc acctacaggg
      961 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     1021 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca cgccttttgc caataatttg
     1081 tctttcatca gcagcggcag cagctcttcc agctcattca ccctcgcatc gaccgcgtgc
     1141 agaaactcct gtttatgttc ctcgtccata ttcttccagg tgttacgcag aaattgttcc
     1201 agtaactgtt gctcaatctc aaacgtagac atctctttgt cggctttcag cttcaatcgt
     1261 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     1321 atactatcgc caccaaaatg ctgtaattct ccagcaatca gctgccagtt gcggcgatgt
     1381 tgctcgggat gcccttccat cgatttaaac agttcgttgc gcatcagtac gctggagagg
     1441 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     1501 ctgcaatgct ggagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     1561 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     1621 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcgct
     1681 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcaggct
     1741 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccgcttaact gtaaatcgcc
     1801 atggtcggca gagagttgta tgcgtttaac aattcggcaa acaggaagtt tcagcgccag
     1861 atcgttggtt tcgttacgtg gcatcgcaat gacgccgagg attttatggt cgtttgcctg
     1921 cgccgtgcag cacagcatca gactaatcgc caggctggcg gaaatcgtaa aaacggattt
     1981 cataaggatt ctcttagtgg ggagaggtag ggggatggat acccactagt ttactgctga
     2041 taaagagaag attcaggtgc gtaatatttt ctttttatta caattttttg atgaatgtct
     2101 tggctgcgat tcattcttta tatgaataaa attgctgtca gtttcacgtc ttgtcctgct
     2161 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     2221 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgtaaaaa aatgaaattg
     2281 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     2341 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     2401 ttgtgtagcg attatggatg gcaccactcc tcgtgtgctg gagaacgccg aaggcgatcg
     2461 caccacgcct tctatcattg cctataccca ggatggtgaa actctggttg gtcagccggc
     2521 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     2581 tcgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     2641 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     2701 gatctctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc
     2761 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     2821 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     2881 agctgcgctg gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga
     2941 cctgggtggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     3001 aaccttcgaa gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag
     3061 ccgtctgatc aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     3121 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     3181 ttccgctcag cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa
     3241 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     3301 ccgttccatt gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat
     3361 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     3421 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     3481 tgctgctgtt cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt
     3541 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     3601 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     3661 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     3721 gggtcagttc aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     3781 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     3841 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     3901 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     3961 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     4021 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac
     4081 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt
     4141 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     4201 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     4261 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     4321 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     4381 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca
     4441 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgaccaagag gccgaggcga
     4501 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     4561 acgatcagta tggtcatgct gcgtttgagc aaggtggtat gggcggcggc ggttttggcg
     4621 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     4681 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     4741 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     4801 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     4861 gttctggtca ggtgcagatg cgtcagggtt tctttgccgt gcagcagacc tgtccacact
     4921 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     4981 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     5041 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
     5101 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg
     5161 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     5221 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc
     5281 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
     5341 tcgtcgaaac accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag
     5401 aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg
     5461 gtgtgaagaa gttttttgac gacctgaccc gctaaccacc ccaaaagcct gcccgtgggc
     5521 aggcttgggt aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat
     5581 cactcccata agcgctaact taagggttga accatctgaa gaatgcgacg cctcggtgcc
     5641 tcgttaagac gatgcctcgc gttcttcaat tgcgttttgt aggctgtcag ggatactgtc
     5701 ccacgaatgg ccacctgtaa gctccagatg accatttttg ttattctcca caacgagtta
     5761 gttcttcttt tcggatccgg cacttctggg ggggaaatcc agcgatggct ggattatgtc
     5821 gtcaattaaa aatgcggcga gtagattagc aaatatccac gctttcgcga gttcaggttc
     5881 ctttgcacgc aaagcatcca ggtgcagcaa acttttgagc cgcttaaaag ccagttcaat
     5941 ttgccatcgc agacggtaac aatcagccac ttgctctgct gaatattcat cttccggtaa
     6001 tgatgttagc aatagcacat ggcccgctgc ttccagcgtt tccgcctgaa ctactcgtcc
     6061 ttttcgacga ttctcgctga gcagtcgggt tttactgatt aatgcttttt cgggaggaag
     6121 tgatacggca atgagacgtg ccggaaaggg agctccggct tttttattac ctgaattgcc
     6181 tatcattaca gtggtttcac cgttcttacc gcaatccagc ccgcgcagaa aacccatcat
     6241 gtcaaagcgc attccttctg cagttaacca gcgcaatcct cgccagtgaa cccggacgat
     6301 ataatcagct tctccaaaag caagtgagcg gatacattcg ggacgcgaac cgaatccccg
     6361 gtcagcaatg cgtatctcgt ctgccgtttg cgcaaatcgg tccagccgtt cagcgtctct
     6421 gctgtcggtt agctcaaaat cagtgaactg acaggtatga ggatcatatc ccatatgtag
     6481 tcgccattca gcgctgccgc ccccgggcgc actgattgct gttccatcga caagacgcaa
     6541 tctctttccg cttgtacaac ccgtaactgc ggcgcgtaca gcaagtgttt gtgcggcaag
     6601 tatgccaaac cagtcggcgg cattccgcag ccgcttcagg agagccacgt cagataatgt
     6661 tgcaacgtca tggagctgag cccatgcagt gacttcacgt aatgacatcc ccccggggcc
     6721 gtaagccagc cccagacgta gcagagttgc agcatcacga atttcgcggc ggcgggttag
     6781 agccccggca ttacgtgccg aagtatccag ttcttcgggc ttaccaatat gggccagaat
     6841 tgctgaccag ttatcgtgag agtaattcat cggcacgtta aatcatatca ggcgtaatac
     6901 c
//
LOCUS       DACAPB010000213         6874 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.214, whole genome shotgun sequence.
ACCESSION   DACAPB010000213 DACAPB010000000
VERSION     DACAPB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.214"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..763
                     /locus_tag="IT904_004122"
                     /pseudo
     CDS             1..763
                     /locus_tag="IT904_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1054..1632
                     /locus_tag="IT904_004123"
     CDS             1054..1632
                     /locus_tag="IT904_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004857495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3193822.1"
                     /translation="MLLPMNTETQHRKKDPIRLYQQLLESAAMIAGRDGIAALSLNAV
                     AREAGVSKGGLLHHFPNKQALIYALFARLLAIMEEAIAALMQKDNISYGRFTRAYVNY
                     LSALTDTQESRQLMVLSLAMPDEPVLRKCWRDWMLGHLANGDELDNSSTGTLVRYTAD
                     GIWLSELTEGITMSPEHRQALVDSLNKMTLPA"
     gene            1655..2473
                     /locus_tag="IT904_004124"
     CDS             1655..2473
                     /locus_tag="IT904_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix family transcriptional regulator"
                     /protein_id="HAP3193823.1"
                     /translation="MRYNINARFIYDATDGTLTLPQSTEPDCQLSITSCALLNFFLHH
                     TDIVSREEVLTKVWDNNGLTSSNSNLNQYLSMLRKTFRHYGIDNIIITVARGYLQLNP
                     NVSIEPLDEAPAPPAIIEPSIIPDVDVPTSSEDTESSPPTLAPVTHRHDAYWYFAGAC
                     LLTISILLVAFNLFGISEARPIALTQLSHSQCELLASDEMRRSVSVKAYEKNYDEVRK
                     RLNIACKPSERFLFFYGDRLETNGLGRVFLAHCAMHENNPFSYCDNYFYYSWKL"
     gene            2473..2991
                     /locus_tag="IT904_004125"
     CDS             2473..2991
                     /locus_tag="IT904_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193824.1"
                     /translation="MRIAPRTFFAISALAFIVASGFSFWRLSPAENTGIMSCSTKGIM
                     RFENMEKENVNGNIHFNFGSQGKGSMVVEGYTDSAAGWLYLQRYVKFTYTSKRVSATE
                     RHYRISQWESSASSIDESPDVIFDYFMREMSDSHDGLFLNAQKLNDKAILLSSINSPL
                     WICTLKSGSKLD"
     gene            3511..3789
                     /locus_tag="IT904_004126"
     CDS             3511..3789
                     /locus_tag="IT904_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAP3193825.1"
                     /translation="MGDALGLIETKGLVACIAAADAMCKSANVELIGYENIGSGLVTV
                     MVKGDVGAVKASVDSGLESAQHIGEVVTSLVIARPHNDINKIVIKHKA"
     gene            3807..4091
                     /locus_tag="IT904_004127"
     CDS             3807..4091
                     /locus_tag="IT904_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAP3193826.1"
                     /translation="MGDALGLIETKGLVACIEAADAMCKAANVELIGYENVGSGLVTA
                     MVKGDVGAVKAAVDSGVESAQRIGEVVTSLVIARPHNDINKIVSHYKITD"
     gene            4106..4384
                     /locus_tag="IT904_004128"
     CDS             4106..4384
                     /locus_tag="IT904_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAP3193827.1"
                     /translation="MREALGLIETKGLVACIEAADAMCKAANVELIGYENVGSGLVTA
                     MVKGDVGAVNAAVDSGVEAARRIGEVVTSRVIARPHNDIEKIASQHKA"
     gene            4438..6024
                     /locus_tag="IT904_004129"
     CDS             4438..6024
                     /locus_tag="IT904_004129"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAP3193828.1"
                     /translation="MIELDTDLRSRQNARVLVRNAKKAQAIMATFSQQQIDAIVKNVA
                     QEAAHHAEALAKMAAEETGFGNWQDKVLKNRFASLRVYDAIKDMKTVGIIHDDQQQKV
                     MDVGVPLGVICALVPSTNPTSTIFYKTLIALKAGNAIIFSPHPGARQCSWKAIEVVKR
                     AAEAAGAPAGIVDGVTELTLEATQELMHSKDVSLILATGGEGMVRAAYASGTPTISGG
                     PGNGPAFIERSADIHHAVKDIITSKTFDNGVICASEQSIIVERCIYNEVHRELEAQGA
                     YFMNEDEAAKMAALLLRPNGTINPKVVGKTALYLSQMAGFCVPASTRVLIAEQTTVSH
                     KNPYSREKLCPVLGLYIEEDWKAACHRVVELLTNEGLGHTLVIHTRNQDVIRQFCLEK
                     PVNRILINTPAALGGIGATTNITPALTLGCGAVGGGSSSDNVGPMNLLNIRKVGYGVR
                     SIDELRSPGSRAEPQPAIAAPIVEPHRSILDDARFTSPAPATTSADDRFTVATTDPEG
                     EINEQNVERVIRQVLERLGK"
     gene            6051..6308
                     /locus_tag="IT904_004130"
     CDS             6051..6308
                     /locus_tag="IT904_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HAP3193829.1"
                     /translation="MILAKVTGHVVATQKCDELRGSNLLLITQLDDNQQLMKDRSWVA
                     VDSVGAGVHDVVLAEEYFALNKDRYKAMSVVAIVEKVFRDA"
     gene            6324..>6874
                     /locus_tag="IT904_004131"
     CDS             6324..>6874
                     /locus_tag="IT904_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP3193830.1"
                     /translation="MSEFLLKPRICFGQDALSVLDELTACHVLLVTDQAMVKFGLAER
                     VTQRLCARGIDWQVWDDVVADPDIATVVRGMKLMDSCYPDLVIALGGGSVIDAAKAVI
                     FALAQTRPEAGRERPCFVAIPTTSGTGSEVTSFSVVKAHAEKLVLVDSSLLPDIAILD
                     PSLVASVPPAITADTGMDVLCHAL"
BASE COUNT         1722 a         1707 c         1789 g         1656 t
ORIGIN      
        1 gataaacgca gtgcacatgc acagcgcgat gactggctga agagagagat acagcgcgta
       61 tacgatgtac ggtgtgcgta aagtctggcg tcagttgtta cgggaaggaa tcagggtggc
      121 cagatgtaca gtggcgcgcc tcatggcggt tatgggactt tccggtgttc tccggggtaa
      181 aaaggtccgc actaccgtca gccggaaaac cgttgccgca ggtgaccgcg taaaccgtca
      241 gtgcgtggca gaacgtcctg accagctgcg ggtggctgat tttacttacg tcagcacatg
      301 gcagggcttc gtctatgtgg cgttcatcat tgatgtgttt accggataca tcgtggggtg
      361 gcgggtatca tcgtccatga aaacgacatt cgtgctggat gcgctggagc aggcgttgtg
      421 ggcccgtcgt ccgtccggca ccatccatca cagcgataaa ggctctcagt atgtgtcact
      481 ggcctatacg gagcgattaa aagaagcaaa actgctggca tcaacaggga gtacaggcga
      541 ctcgtatgac aacgcgatgg cggagagcat caatggtctt tacaaagcgg aggtaataca
      601 ccgtaagagc tggaaaaacc gtgcagaagt ggaactggtc acactcacgt gggtggactg
      661 gtataacaat cgacgattgc tgggaaggct gaggcatact cctccggcag aagcagaaaa
      721 agcgtattat gcttccatcg gaaatgatga gctggcagcc tgagttcaca gataaaacac
      781 tctccaggaa ccccggggcg gttcattctc gcttgccttc tgtatttcaa aaagcatcat
      841 tcaggtcatt aaaagttatt tgatcatctt tttatggatt ttctgttcat cgaaagggta
      901 acagattccc accattgtgc agaccaggag aattaacagc agcagtagga tttatacgac
      961 aaattgtgac ctcacacgca atacccatac tcacttcatg ggtagctgct tctccttgat
     1021 agcgatcaaa aaaaaacata ccgtctggtc ggtatgcttc tgcctatgaa tacagaaacc
     1081 caacatcgta aaaaagatcc catccgtctg tatcagcagt tgctggagtc tgcagctatg
     1141 attgctggtc gtgacggtat cgctgccctc tcgctgaatg cggttgcccg cgaagctggc
     1201 gtgagtaaag gtggattact gcatcacttt ccgaacaagc aggcgctgat ctacgccttg
     1261 tttgcccggt tgttggcaat aatggaagag gccatcgctg ccctgatgca aaaagacaat
     1321 ataagctatg gccgttttac tcgcgcatat gtgaactatc tttctgcgct gactgacaca
     1381 caagaaagcc gtcaattgat ggtgctttcg ctggcaatgc ccgatgaacc ggtgctgcga
     1441 aaatgctggc gagactggat gcttggtcat ttagcgaatg gcgatgaact ggataatagc
     1501 tcaaccggaa cgctggtgcg ctataccgca gatggcatct ggttgtctga gttaacggaa
     1561 gggatcacca tgagccccga acatcgtcag gcactggttg attcactgaa taagatgaca
     1621 cttcccgcgt gaagtcccat ggattgaggt cgcgatgcgc tataacatca atgcccgttt
     1681 catttatgac gctacggatg gcacgcttac gctaccgcaa agcactgagc cagactgcca
     1741 actctcgata acgtcctgcg cgctgctcaa cttttttttg catcataccg atatcgttag
     1801 tcgcgaagag gtactgacaa aagtttggga taacaacgga ttaacttctt ctaacagcaa
     1861 tctgaatcag tatctcagta tgctgcgcaa gacgttccgt cactatggta ttgataatat
     1921 tatcattacc gttgcacgcg gctatctgca gctaaacccg aatgtatcca tcgaaccact
     1981 ggatgaagcc cctgcgccac ctgcaattat agagccatcc ataatccctg atgttgacgt
     2041 accaacatcc agtgaggata ccgagtcttc accaccaaca ttagcgccag tcacccatcg
     2101 tcacgacgct tactggtatt ttgcaggtgc ctgtttgtta accatttcga ttctgttggt
     2161 agccttcaat ttgttcggta tcagcgaggc gcgccccatc gctttgacgc aactttctca
     2221 tagccagtgc gaattactgg caagcgacga aatgcgccgt tcagtaagcg tgaaggcata
     2281 tgagaaaaat tatgacgaag tccgtaagcg tctaaatatc gcctgtaaac caagtgagcg
     2341 ttttcttttt ttctacggtg acaggctgga aaccaacggc cttgggcgag tgttcctcgc
     2401 gcactgcgct atgcatgaaa ataacccgtt tagttattgc gataactact tttattactc
     2461 ttggaaactg taatgagaat cgcaccgcgt accttctttg ctatttccgc cctggcgttt
     2521 attgtcgcct ccggatttag tttctggcgg ttgtcccctg ctgaaaatac agggattatg
     2581 agttgttcaa caaaaggcat catgcgtttt gagaatatgg aaaaggagaa cgttaacggt
     2641 aatattcact ttaactttgg cagccagggt aaaggttcga tggtggtcga aggctacacg
     2701 gactctgccg ctggctggct gtacctgcaa cgctatgtca aatttaccta taccagtaaa
     2761 cgtgtttccg ccacggaacg ccattaccgc atcagccagt gggaatccag cgcctcatcg
     2821 atagatgaat caccagatgt gatttttgac tactttatgc gtgaaatgtc tgacagccat
     2881 gacgggctgt tcctcaacgc ccagaagctg aacgataaag cgattttgct cagttctatt
     2941 aattcaccgc tttggatctg tacccttaaa tctggcagca aattagacta gttttttctg
     3001 gctgccttac gcaacgaatt gctcaccact ttcatcgtcc ccccttcgcc tgacgagaaa
     3061 cacgactctt taacttcgtg atagcacagg cgatttgtga tgtaccacgc attccatttt
     3121 ccccttctca acggccctga gcaaaagcgc attttttgcg tttagggttt gcgccaaagt
     3181 atgagcccca acacacttca ccgctgttgc tgttaccccc ttttcgtaat tatttagcaa
     3241 cttagcaaca aacatacccg gcgaaaaaaa tacgccaaaa agcgcattat taaaaacaaa
     3301 atcctcccca ccactccagc acaaatacat aaatcagagt gtattgaaag ctaaatttca
     3361 gaatttaatt ctaaatcatt gctgtcagca gatttaaatt ccacaaaatt ccatgagctc
     3421 tatagtgaca ttcgaacgcc gcttccgtga gttaacgctg tgtcacggac ggttttcctg
     3481 aatcacaaaa cctgttctga ggagatcacc atgggtgatg cattagggct tatcgaaacc
     3541 aaaggcctgg tggcctgtat tgccgcagcg gatgcgatgt gtaaatccgc caacgttgaa
     3601 ctgattggct atgaaaacat cggctctggt ctggtgaccg tgatggtgaa aggcgacgtt
     3661 ggcgcagtga aagcatctgt cgattctgga cttgagtccg cacagcacat cggggaagtg
     3721 gtgacgtcgc tggttatcgc gcgtcctcac aacgacatca acaaaatcgt tattaaacat
     3781 aaagcttaag gccggaggga agaaacatgg gtgatgcatt aggtctgatt gaaaccaaag
     3841 gtttggtggc ttgtattgaa gcagctgacg cgatgtgtaa ggccgccaac gttgagttga
     3901 ttggctatga aaacgtcggt tctggtctgg tcaccgcaat ggtgaaaggt gatgtcggcg
     3961 cagtaaaagc ggcggtggat tccggtgttg agtccgcaca gcgcattggc gaggtggtga
     4021 cgtcgttggt tatcgcgcgt ccacacaatg acatcaacaa gattgtctcg cactacaaaa
     4081 ttaccgatta accggagaaa acataatgag agaagcgctt ggtctgatcg aaaccaaagg
     4141 tctggtggcc tgtattgagg cggcagacgc gatgtgtaaa gccgccaacg ttgagttgat
     4201 tggctatgaa aacgtcggct ctggcctagt caccgcgatg gtaaaaggcg acgttggcgc
     4261 agtgaatgcc gcggtggatt ccggtgttga agcagcaaga cgtatcggtg aagtggtgac
     4321 ctcccgtgtc atcgcccgcc cgcataacga catcgaaaaa atcgcgtcgc agcataaagc
     4381 atgacctgac agggcacgcc ccgtctgcac tcttttagtc tgatgaagga tagactcatg
     4441 attgaactgg ataccgattt gcgctcccgt cagaacgcgc gggtgctggt gcgtaacgcc
     4501 aaaaaagcgc aagcgattat ggccactttt tcgcagcagc aaatcgacgc catcgtgaaa
     4561 aacgtggccc aggaagcagc acaccacgct gaagcactgg caaaaatggc cgcagaagaa
     4621 actggcttcg gcaactggca ggataaagtc ctgaaaaacc gtttcgcctc gctgcgtgtt
     4681 tacgatgcca tcaaagatat gaagaccgtc ggcatcattc atgacgatca acagcaaaaa
     4741 gtgatggacg tgggcgtgcc gctgggtgtg atttgcgccc tggtgccgtc aaccaacccg
     4801 acttccacca tcttctacaa aacgctgatc gccctgaaag ccggtaatgc gattattttc
     4861 tctccgcatc ccggcgcacg tcagtgtagc tggaaggcga tagaagtcgt taagcgtgcc
     4921 gcagaagctg ctggtgcacc tgcgggtatt gtcgatggtg taaccgagct cacgctggaa
     4981 gcaacccagg aactgatgca tagcaaagat gtttcgctga ttctggctac cggtggtgaa
     5041 ggaatggtac gagcagcata tgcttctggc acaccgacca tcagcggtgg tcctggtaac
     5101 ggtccggcat ttattgaacg cagtgcggat atccaccatg cggtgaaaga tatcatcacc
     5161 agcaaaacct tcgataatgg tgtgatctgc gcttctgaac agtcgattat tgttgaacgc
     5221 tgcatttaca acgaagtaca ccgcgaactg gaagctcagg gtgcttactt tatgaacgaa
     5281 gatgaagcgg caaaaatggc agctctgctg ctgcgcccga acggcacgat taacccgaaa
     5341 gtggtgggaa aaaccgcact gtatttaagc cagatggccg gtttttgtgt gcccgccagt
     5401 accagagtac tgattgccga acaaactacc gtttctcaca aaaaccccta ctcacgtgaa
     5461 aagctgtgcc cggttcttgg tctgtatatc gaagaggact ggaaagctgc ctgccaccgc
     5521 gtggtggagt tgctgactaa cgaaggtctg ggccacacac tggtgatcca tacccgcaac
     5581 caggatgtga tccgtcaatt ctgccttgaa aaaccggtta accgcattct gatcaatacc
     5641 cctgcggcgc ttggcggtat tggcgctacc actaacatta cccccgccct gacgcttgga
     5701 tgcggtgcgg taggtggtgg ttcctcttcc gataacgtcg ggccaatgaa tctgctcaac
     5761 attcgtaaag tgggctatgg cgtgcgttcc atcgatgagt tgcgttctcc gggtagccgt
     5821 gcagaaccgc agccagccat cgccgcgccg atagtggaac cgcatcgcag cattcttgac
     5881 gatgcccgct tcacctcccc tgccccggca accacatctg ctgatgatcg ttttactgtg
     5941 gcaacgactg atcccgaagg cgagatcaac gagcagaacg ttgagcgagt catccgccag
     6001 gtgcttgagc gcttaggcaa gtaattcatt gagataaggc gataaaaacg atgattctcg
     6061 caaaggtaac cggacatgtc gtcgccacgc aaaagtgcga tgagctgcgc ggcagcaacc
     6121 tgttgttgat cacccaactg gatgataacc aacagctgat gaaggaccgc tcctgggtcg
     6181 ctgtcgatag cgtcggtgca ggcgttcatg acgttgtgct ggcggaggag tacttcgcac
     6241 tcaataaaga tcgttacaaa gcgatgtcag tggtcgccat tgtcgagaag gtttttcggg
     6301 acgcttaagg agtgaaaaaa ccaatgagtg agtttttgct gaaaccccgg atctgcttcg
     6361 gtcaagacgc gctgtcagtc ctggacgagc taaccgcctg tcatgttttg ctggttaccg
     6421 atcaggcgat ggtcaaattc ggtcttgctg aacgtgtcac gcagcgtctc tgtgcgcgcg
     6481 gcattgactg gcaggtgtgg gatgacgttg tcgccgatcc ggatatcgct accgtggtcc
     6541 gagggatgaa gttgatggac agctgctacc cggatctggt tatcgcactc ggcggtggtt
     6601 cggtcattga cgcagccaaa gcggttattt tcgccctggc gcaaacccgt ccggaagcag
     6661 gacgtgaacg cccctgcttt gtggctattc caaccaccag cggtacgggc tcagaagtga
     6721 ccagtttctc ggtggtgaaa gcccatgccg aaaaactggt gctggttgac tcgtcgctgc
     6781 taccggatat cgccattctt gatccgtcac tggtggcatc agtgccacct gctatcaccg
     6841 ctgacaccgg aatggatgtg ttgtgccacg ctct
//
LOCUS       DACAPB010000214         6867 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.215, whole genome shotgun sequence.
ACCESSION   DACAPB010000214 DACAPB010000000
VERSION     DACAPB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.215"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1612)
                     /locus_tag="IT904_004132"
     CDS             complement(<1..1612)
                     /locus_tag="IT904_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP3193831.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDED"
     gene            complement(1832..5515)
                     /gene="metH"
                     /locus_tag="IT904_004133"
     CDS             complement(1832..5515)
                     /gene="metH"
                     /locus_tag="IT904_004133"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP3193832.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALSFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            5715..6539
                     /gene="iclR"
                     /locus_tag="IT904_004134"
     CDS             5715..6539
                     /gene="iclR"
                     /locus_tag="IT904_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAP3193833.1"
                     /translation="MVAPIPAKRGRKPAAATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         1598 a         1910 c         1761 g         1598 t
ORIGIN      
        1 catcttcatc cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc
       61 gctgcatatc tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt
      121 gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat
      181 gtttgctgcg acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg
      241 ccagttttaa attactgagc aattgctcat agagcgcatc cagttccttc aagccatcaa
      301 gcgaaaatgc ccgccgtgct gccagcgatt tatcagcaat ttcgctgccc atgcgctcga
      361 cgatatcgga agcctgttca aggttgagcg acatttcaat aatctccgcc cagcgacgcg
      421 actcttcttc tgccagttcc tcttttggca tccgcgccag atacagctta atggcggtat
      481 agagaacgtt gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt
      541 gcatcacttt attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt
      601 cgcgcgcggc gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt
      661 taggccgtag ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg
      721 ccatcgggtc aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga
      781 aatagatcac cagttccgct tttggcaatg acaacttccc cattgtctct gccagcaaat
      841 ggacaaacgg caggataatc aggctaccca ccagcttaaa cagcaaacta cccagcgcga
      901 cacggcgggc tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga
      961 ggttagcacc aatcaccaga cagagcgcca cggggaagga gataatgcct gcggcggtca
     1021 gagtcgcagt cagcaataca gcagcaaggc tggagtagct gataatggcg aacatcgcgc
     1081 caatcagcgc atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt
     1141 ttgcctgggt gatcggcgtt acggcctgca caatcaactc cagcgctagc aaaatcagcc
     1201 caagaccaat accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa
     1261 aaatcacgcc gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac
     1321 gcgccattag cgccgtcccg acatctgcac ccagcacaat gaccagagcc ggtgcgaggg
     1381 ctaccagatc ctgagcgaca aacgaggtca ccagcatggt ggtggcatta ctgctctgta
     1441 ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc
     1501 taaggacagt acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag
     1561 tcccccagac cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag
     1621 gttctcctta tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc
     1681 tgactggcat ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc
     1741 tgtttccagt ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt
     1801 ttgttgtaag gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg
     1861 ccagccagcg ctcaacttcg gtaacgctca tacctttacg gcgggcataa tcttcaacct
     1921 gatcgcgctg aatttgtgct acagcgtagt acttgctgtc cgggtggctg aagtaccaac
     1981 ccgaaaccga tgcaccgggc cacatggcga aagattctgt gagtttcatg ccagtgtgtt
     2041 tttccacttc cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat
     2101 agcccggtgc cggacggatg ccctggtagt tttcgcggat cagctcttcg ttgctgaggt
     2161 tctcgttcgg cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa
     2221 aggcttcggc taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct
     2281 gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc
     2341 cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag
     2401 cgaagcctgt tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtac
     2461 gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca
     2521 ccacgccacg cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt
     2581 ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact
     2641 tcccggccag cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt
     2701 cgatgctggc ttcgacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct
     2761 gccagtcaaa agcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgcg
     2821 gtttcttgcg cccgtgctga atacgtacgg tttcgtactc cttgcgggta cgagcgacaa
     2881 aatcatcacg ctgggtatcg gaaagcagcg ccgccaccac accaacggta cgcgaggcat
     2941 tctgcacata caccgtcggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt
     3001 ttgaggtcgt cgcgccgcca atcaatagcg gaatagtgaa gccctgacgc tccatctctt
     3061 tcgccacgtt aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcagat
     3121 cagcattcac ttctttagcg gtacggagaa ttttttccgc aggcaccata acgccgagat
     3181 cgacaatttc gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt
     3241 ggacgtcgcc cttcacggtg gcgatcacca tcttgccgtt ggttttgccc tgctctttgc
     3301 tggcttcaat aaacggttcg aggtaggcca ccgcctgttt catgacgcgc gccgatttga
     3361 ccacctgtgg caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt
     3421 ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcgg
     3481 tatcctgctc gataaactca gtaatgcctt tgaccagcga gtattccaga cgtttattca
     3541 cttcccacga gcgccactcc gcctgctggg cgttagcggt gtcgtcggtt ttgctgccgc
     3601 gatatttctc ggcaagctcc agtaaacgct cagtgccatc atcgcgacga ttaagaatca
     3661 catcttccac cgcgtcgcgc agttcagcgg gcaggtcgtc gtaaatcgcc agctgcccgg
     3721 cattgacgat ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa
     3781 tggcttcgcg caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg
     3841 aaatcagcgc gtgcggcagt tcgcgtttga tgtcttcgca ggcgccgata aagtcctgcg
     3901 catagttgtt gtgctcttca atgccggttg cgaccgcgaa gatgttcggg tcgaagatga
     3961 tatcttctgg cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct
     4021 cgattttccg tgcgcgggta tcggcctgtc cctgttcgtc aaaggccatt accaccactg
     4081 ccgcaccgta acgacgcaag agcttcgcgt gatggataaa ggcgtcgacg ccctctttca
     4141 tcgagataga gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat
     4201 cccattttga ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca
     4261 gattgagaaa acgcaccatc gccgcttcgg cgtcgagcat cccttcatcc atgttgatat
     4321 cgataatctg cgcaccgttt tccacctgct ggcgggccac atccagtgcc tcgctgtatt
     4381 tctcttcttt gatcaggcgc ttgaacttag cggaaccggt gacgttggtg cgttcaccca
     4441 cgttcacaaa caggctatct tcgccaatgt tcagcggctc caggccggac aaacggcagg
     4501 ctacggggat ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg
     4561 caatatgttg tggcgtggtg ccacagcagc cgccgacgat attgagaaaa cccgcttgcg
     4621 cccattcacg tatctgtttt gccatcgtgt cggcgtcgag atcgtactca ccaaaggcgt
     4681 tgggtagccc ggcgttcggg tgcgcggtga cgtagcattc cgcaatccgt gacagctcct
     4741 gcacgtactg gcgcagttca tcgggcccca gcgcacagtt caggccaaag ctcagagctt
     4801 cggcgtggcg caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg
     4861 aggcgtcggt gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact
     4921 ccgttttcac cgcaaaaacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca
     4981 ggatcagatc cgcgccacct tccaccagcg ctttggtgga ctctcgataa gccgccacca
     5041 gcccgtcaaa agtgatatta cgaaatgccg gatcatttac gtccggagaa atagacgccg
     5101 tgcggttggt cgggccgaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg
     5161 tccactcatc agcacaagct cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca
     5221 gggattccat ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga
     5281 tgatatccgc gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttgc
     5341 tgagcaccag caggtcgttg ttgcctttga gatcgcatgg ccagtcggca aagcgttcac
     5401 cacgaaaatc ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca
     5461 gcaccagaat acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg
     5521 ctcccgacac gctcaaccca gatttaataa aaattcaaca aaccatactg gcataaacgc
     5581 atctgtggta aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat
     5641 cagttcagca actattgcat tagctaacaa taaaaatgaa aatgatttcc acgatacaga
     5701 aaaaggaggc tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgctg
     5761 ccaccgcacc agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt
     5821 ggattgccga atccaatggc agtgtggcac tcacagagct ggcgcaacaa gccgggttac
     5881 ccaattccac gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg
     5941 tcggcgaact gggacattgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc
     6001 tccagagccg taatttgtta gcgattgttc accctatcct gcgcaattta atggaagagt
     6061 ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg
     6121 accaggtaca gtgtacgcat ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga
     6181 tgcacgcttc cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga
     6241 agctgctgca ccgcaaaggg ttacatgcct atacccacgc aacgctggtg tctcctgtgc
     6301 atttaaaaga agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac
     6361 atgcactggg gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg
     6421 ccgcaatttc aatttccgga ccgatttcac gtattaccga tgaccgcgtg accgagtttg
     6481 gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac
     6541 tttttctggc gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg
     6601 gtgtctgtta atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta
     6661 ttaactcagc attattttta aacatcaaac cacttaatca ttaacaaaat cgcaagatct
     6721 cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga tatttcttta
     6781 gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga catcaataaa
     6841 caatgagata tttaaatgat tactcgt
//
LOCUS       DACAPB010000215         6847 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.216, whole genome shotgun sequence.
ACCESSION   DACAPB010000215 DACAPB010000000
VERSION     DACAPB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.216"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            147..221
                     /gene="tnaC"
                     /locus_tag="IT904_004135"
     CDS             147..221
                     /gene="tnaC"
                     /locus_tag="IT904_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAP3193834.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            442..1857
                     /gene="tnaA"
                     /locus_tag="IT904_004136"
     CDS             442..1857
                     /gene="tnaA"
                     /locus_tag="IT904_004136"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAP3193835.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1948..3195
                     /gene="tnaB"
                     /locus_tag="IT904_004137"
     CDS             1948..3195
                     /gene="tnaB"
                     /locus_tag="IT904_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAP3193836.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3328..4503
                     /gene="mdtL"
                     /locus_tag="IT904_004138"
     CDS             3328..4503
                     /gene="mdtL"
                     /locus_tag="IT904_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAP3193837.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4478..5437
                     /gene="yidZ"
                     /locus_tag="IT904_004139"
     CDS             4478..5437
                     /gene="yidZ"
                     /locus_tag="IT904_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAP3193838.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5594..6343
                     /locus_tag="IT904_004140"
     CDS             5594..6343
                     /locus_tag="IT904_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193839.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6365..>6847
                     /gene="yieF"
                     /locus_tag="IT904_004141"
     CDS             6365..>6847
                     /gene="yieF"
                     /locus_tag="IT904_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAP3193840.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     G"
BASE COUNT         1609 a         1666 c         1705 g         1867 t
ORIGIN      
        1 tcttacgtaa tttataatct ttaaaaaaag catttaatat tgctccccga acgattgtga
       61 ttcgattcac atttaaacaa tttcagaata gacaaaaact ctgagtgtaa taatgtagcc
      121 tcgtgtcttt cgaggataag tgcattatga acatcttaca tatatgtgtg acctcaaaat
      181 ggttcaatat tgacaacaag attgtcgatc accgcccttg atttgccctt ctgtagccat
      241 caccagagcc aaaccgatta gattcaatgt gatctatttg tttgctatat cttaattttg
      301 ccttttgcaa aggccatctc tcgtttattt acttgtttta gtaaatgatg gtgcttgcat
      361 atatatctgg cgaattaatc ggtatagcag atgtaatatt cacagggatc actgtaatta
      421 aaataaatga aggattatgt aatggaaaac tttaaacatc tccctgaacc gttccgcatt
      481 cgtgttattg agccagtaaa acgtaccact cgcgcttatc gtgaagaggc aattattaaa
      541 tccggtatga acccgttcct gctggatagc gaagatgtgt ttatcgattt actgaccgac
      601 agcggcaccg gggcggtgac gcagagtatg caggccgcga tgatgcgcgg cgacgaagct
      661 tacagcggca gccgcagcta ctatgcgtta gccgagtcag taaaaaatat ctttggttat
      721 caatacacta ttccaactca ccagggccgt ggtgcagaac aaatctatat tccggtactg
      781 attaaaaaac gcgagcagga aaaaggcctg gatcgcagca aaatggtggc gttctctaac
      841 tatttctttg ataccacgca gggccatagc cagattaacg gctgtaccgt gcgtaacgtc
      901 tatatcaaag aagccttcga tactggcgtg cgttacgact ttaaaggtaa ctttgacctc
      961 gaaggattag aacgcggtat tgaagaagtt ggcccgaata acgtgccgta tatcgttgca
     1021 accatcacca gtaactctgc aggtggtcag ccggtttcac tggcaaactt aaaagcgatg
     1081 tacagcatcg cgaagaaata cgatattccg gtggtaatgg actccgcacg ctttgctgaa
     1141 aacgcctatt tcatcaagca gcgtgaagca gaatacaaag actggaccat cgagcagatc
     1201 acccgcgaaa cctacaaata tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg
     1261 gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct tctttgatgt gtacaccgag
     1321 tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat atggcggcct ggaaggcggc
     1381 gcgatggagc gtctggcggt aggtctgtat gacggcatga atctcgactg gctggcttat
     1441 cgtatcgcgc aggtacagta tctggtcgat ggtctggaag agattggcgt tgtctgccag
     1501 caggcgggcg gtcacgcggc attcgttgat gccggtaaac tgctgccgca tatcccggca
     1561 gaccagttcc cggcacaggc gctggcgtgc gagctgtata aagtcgccgg tatccgtgcg
     1621 gtagaaattg gctctttcct gttaggccgc gatccgaaaa ccggtaaaca actgccatgc
     1681 ccggctgaac tgctgcgttt aaccattccg cgcgcaacat atactcaaac acatatggac
     1741 ttcattattg aagcctttaa acatgtgaaa gagaacgcgg cgaatattaa aggattaacc
     1801 tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa aactgaaaga agtttaatta
     1861 atactacaga gtggctataa ggatgttagc cactctctta ccctacatcc tcaataacaa
     1921 aaatagcctt cctctaaagg tggcatcatg actgatcaag ctgaaaaaaa gcactctgca
     1981 ttttggggtg ttatggttat agcaggtaca gtaattggtg gaggtatgtt tgctttacct
     2041 gttgatcttg ccggtgcctg gtttttctgg ggtgccttta tccttatcat tgcctggttt
     2101 tcaatgcttc attccgggtt attgttatta gaagcaaatt taaattatcc tgtcggctcc
     2161 agttttaaca ccatcaccaa agatttaatc ggtaacacct ggaacattat cagcggtatt
     2221 accgttgcct tcgttctcta tatcctcact tatgcctata tctctgctaa tggtgcgatc
     2281 attagtgaaa cgatatcaat gaatttgggc tatcacgcta atccacgtat tgtcgggatc
     2341 tgcacagcca ttttcgttgc cagcgtattg tggataagtt cgttagccgc cagtcgcatt
     2401 acctcattgt tcctcgggct gaagattatc tcctttgtga tcgtgtttgg ttctttcttc
     2461 ttccaggtcg attactccat cctgcgcgat gccaccagca ccactgcggg aacgtcttac
     2521 ttcccgtata tctttatggc tttgccggtg tgtctggcgt catttggttt ccacggcaat
     2581 attcccagcc tgattatttg ctatggaaaa cgcaaagata agttaatcaa aagcgtggta
     2641 tttggttcgc tgctggcgct ggtgatttat ctcttctggc tctattgcac gatggggaat
     2701 attccgcgcg aaagctttaa ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg
     2761 aaatcgttcc tcggcaccaa acagcacggc attatcgagt tttgcctgct ggtgttctct
     2821 aacttagctg ttgccagttc gttctttggt gtcacgctgg ggttgttcga ttatctggcg
     2881 gacctgttta agattgataa ctcccacggc gggcgtttca aaaccgtgct gttaaccttc
     2941 ctgccacctg cgttgttgta tctgatcttc ccgaacggct ttatttacgg gatcggcggt
     3001 gccgggctgt gcgccaccat ctgggcggtc attattcccg cagtgcttgc aatcaaagct
     3061 cgcaagaagt ttcccaatca gatgttcacg gtctggggcg gcaatcttat tccggcgatt
     3121 gtcattctct ttggtataac cgtaatttta tgctggttcg gtaacgtctt taacgtgtta
     3181 cctaaatttg gctgatccct tcaagaagcc agccatttcg ctggcttctt gcctgtcagg
     3241 aaatctcttt tgtccaaatg gcaactcgcc tgattctcct tcaccacgta tgctttgcgt
     3301 caccttacta tcaggacgct ttagcccatg tcccgctttt taatttgtag ttttgccctg
     3361 gttttacttt atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc
     3421 gatctcaatg ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg
     3481 gcagctgcga tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc
     3541 atacccggcg cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc
     3601 acgttatttc ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtagtg
     3661 gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta
     3721 ctcaacggta ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg
     3781 cttaaattcc cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg
     3841 atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa
     3901 ccacgagaaa atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc
     3961 accctcagcg tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa
     4021 atcatgggtt ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc
     4081 atgaccgttt cattctccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg
     4141 atcacctcgc aggtgttatt ccttgcagcg gggatcaccc ttgccgtttc accttcccat
     4201 gcggtttctc tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggcgta
     4261 gcgatgagtc aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta
     4321 ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc
     4381 ggcgcatgga atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg
     4441 attatgttcg tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct
     4501 tgatctcaat ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc
     4561 ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc
     4621 gtggtttgac gacccgctct ttgtgaactc accgctgggc ctgtcgccca caccgctaat
     4681 ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa
     4741 accgcaccac cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat
     4801 gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat
     4861 caaactacgt aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat
     4921 tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt
     4981 agccattgat tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca
     5041 tccggcactg catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat
     5101 ttgctgggaa cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga
     5161 acgcacgatt gctatgagcc tgccggaatt tgagcagtca ctgtttatgg cagcgcaacc
     5221 cgacaatctg ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca
     5281 actgccgttg gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt
     5341 tccttttacc ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg
     5401 ggaaaccatt aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc
     5461 aatttcacaa taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg
     5521 ctttagtctg tccatcattc cagtaaatga ttactcttat attcataatg gaccattaag
     5581 catggagcga aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat
     5641 ttctgttcgt ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca
     5701 aagccgtttt ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat
     5761 tggcgaattg ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa
     5821 tttgccttcg ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga
     5881 aggtgaatgt ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca
     5941 cgcgcccgat aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga
     6001 tcctaacgaa gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg
     6061 tgatgttttg aatgacgatc cgcgcgatct gcagctgctg cccattgccg gacgcctgaa
     6121 atgtgctcat gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tagtgtggtc
     6181 cgtggcggtc acgcccgcga ttgaaaagct cagtgtctgg gagttagatg gcaaacacag
     6241 ctggaaaagc ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg
     6301 ttttgcccag ctctctaccg tgaagtcttt ttcgccaaat tgatagacaa ccacaggagt
     6361 cattatgtct gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt
     6421 taatggcatg gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aagtcaatgc
     6481 gttaccttcc attgccgaca ttcccctgta tgacgctgac gtacagcagg aagaaggttt
     6541 tccagcaacg gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt
     6601 cacgccggaa tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc
     6661 ccgcctgccg gatcaaccgc tggcgggtaa accggtgttg attcagacca gctcaatggg
     6721 cgtgattggc ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc
     6781 aatggtgatg aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatccgca
     6841 aaccgga
//
LOCUS       DACAPB010000216         6748 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.217, whole genome shotgun sequence.
ACCESSION   DACAPB010000216 DACAPB010000000
VERSION     DACAPB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.217"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            165..343
                     /locus_tag="IT904_004142"
                     /pseudo
     CDS             165..343
                     /locus_tag="IT904_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001660165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid/phospholipid synthesis protein"
     gene            465..674
                     /locus_tag="IT904_004143"
     CDS             465..674
                     /locus_tag="IT904_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
                     /protein_id="HAP3193841.1"
                     /translation="MWTSSRVRAVPCLHLSRAGVDARGNAVSQTALHVVAAGPCPLFC
                     PVPFTCLSPCRRGLIQPQPSPPVQG"
     gene            908..1447
                     /locus_tag="IT904_004144"
     CDS             908..1447
                     /locus_tag="IT904_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP3193842.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            1515..1748
                     /locus_tag="IT904_004145"
     CDS             1515..1748
                     /locus_tag="IT904_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP3193843.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAIVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1776..1973
                     /locus_tag="IT904_004146"
                     /pseudo
     CDS             1776..1973
                     /locus_tag="IT904_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2028..2462
                     /gene="psiB"
                     /locus_tag="IT904_004147"
     CDS             2028..2462
                     /gene="psiB"
                     /locus_tag="IT904_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP3193844.1"
                     /translation="MKTELTLNVLQTMNTQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGSQA"
     gene            2459..3178
                     /locus_tag="IT904_004148"
     CDS             2459..3178
                     /locus_tag="IT904_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP3193845.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(3190..3378)
                     /locus_tag="IT904_004149"
     CDS             complement(3190..3378)
                     /locus_tag="IT904_004149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HAP3193846.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            3458..3616
                     /locus_tag="IT904_004150"
     CDS             3458..3616
                     /locus_tag="IT904_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP3193847.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(4237..4473)
                     /locus_tag="IT904_004151"
                     /pseudo
     CDS             complement(4237..4473)
                     /locus_tag="IT904_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4537..4824
                     /locus_tag="IT904_004152"
     CDS             4537..4824
                     /locus_tag="IT904_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193848.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            4942..5763
                     /locus_tag="IT904_004153"
     CDS             4942..5763
                     /locus_tag="IT904_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP3193849.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRIRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(6060..6662)
                     /locus_tag="IT904_004154"
     CDS             complement(6060..6662)
                     /locus_tag="IT904_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP3193850.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
BASE COUNT         1559 a         1702 c         1910 g         1577 t
ORIGIN      
        1 ggtctgatta tcggtctgcg accacggtcc cactcatatt gttagtctga taatcagtct
       61 gggaccgcgg tcccactcgt attgttggtc tgctaatcag tctgggacca cggtctcact
      121 tgtattgtcg atctgattat cagcgtgaga ctacgattcc attaatgcct gccagtgagt
      181 gtgtggaaca tatcttggcc agtggcttgg tctgtgatgt gctgcgtata ccgggtgaac
      241 cgccccgccg cttgtttgac cgctatattc tgcgggagtt ttcttggagg cacgggatga
      301 aatccgcagc atggcggatg ccctggcaaa aatccggaaa tgatgacgat ggcggggaaa
      361 atccccgcct ttttcctgcc ggtccccgga cagagcacaa aggaagtcgc cgccggctcc
      421 gctttacccg gccatgcgga gcatggcctt gtgggggttc agttatgtgg acgtcgagtc
      481 gtgtgcgggc tgtgccgtgc ctccatctta gccgggctgg cgttgatgca aggggcaacg
      541 ccgtttcgca aaccgcgctt cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg
      601 tgccattcac ctgtttaagc ccctgtcgcc gggggctgat acagccacaa ccgtctccgc
      661 cagtccaggg gtgaaatgta catcccgcaa aactttttgc ccagaagcag gcaaaaactt
      721 ttgcggtctg cccccggaca gtcgtttccg gccgtggcag tgatgcccct tgcggcgaag
      781 gttctcaaaa aggtgatggt gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt
      841 caaaaccgcg gctcccgaaa ctcaaaagat gatgggggct gaaaacatca acagaaggag
      901 gcacatcatg gcagttcgtg gcattaacaa ggtcattctc gtgggacgcc tgggaaaaga
      961 tccggaagtc cgttacatcc ccaacggggg cgcagtggca aacctgcagg tcgccacgtc
     1021 ggaaacctgg cgtgacaaac agacagggga gatgcgggag cagacagaat ggcatcgcgt
     1081 ggtgctgttc ggcaagctcg cggaagtggc tggcgaatat ctgcgcaagg gcgcgcaggt
     1141 ctacatcgaa ggtcagcttc gcacccgtag ctgggaggat aacggtatca cccgttacgt
     1201 caccgaaatt cttgttaaga ccacgggcac catgcagatg ctgggacgtg ccgcaggtgc
     1261 tcagactcag ccggaagagg ggcaacagtt cagcggtcag cctcagccgg aaccacaggc
     1321 ggaggccggt acgaaaaaag gtggcgcaaa aacgaaaggc cgtggacgta aggccgcgca
     1381 gccggagcct cagccgcaac cgccggaggg tgacgattac gggttttcag acgatatccc
     1441 gttctgatcg ggctgactgt gataaccgcc ccgccccgcc ccgttctgtg cggggcatca
     1501 ccggagagat gaggatgagc gaatatttca gaatacttca ggggctgcct gacggtcctt
     1561 ttacccgcaa acatgccgaa gctgttgccg cacagtaccg gaacgtcttt atcgaggatg
     1621 accacggcga acagtttcgc ctggttgtcc gtaataacgg tgcaatagtc tggcgtacct
     1681 ggaattttga ggacggtgcc gggtactgga tgaaccatgt catcagggat ttcgggattc
     1741 ttaagtaaga gaggtgcccg acgcgcgaac gtccgccgca gattatcgca gccctgaata
     1801 aggcagggct gtccggtgcc gcacgggacg cggagaagat gaagaaaggt gatgcggctg
     1861 aacatgcaga gcaccatatg aaagacaacc gctgggttcc aggctggatg tgtgcaccac
     1921 gcccacagac ggatgccact gaacgcaccg ataacctggc tgatgccgcc tgatgaacaa
     1981 ccacaccgcc ccgccggaga cggggcggca gcaagggaga taccgtgatg aaaactgaac
     2041 tgaccctgaa tgtattacag accatgaaca cacaggaata tgaagatatc cgggctgccg
     2101 gaagtgatga acgccgtgag ctgactcacg ccgtgatgcg ggagctggat gcgccggata
     2161 actggacgat gaacggcgag tacggcagcg agtttggcgg ctttttcccc gtccaggtcc
     2221 gtttcacgcc tgcccacgaa cgttttcacc tggcgttatg ttcaccgggc gatgtctctc
     2281 aggtctgggt gctggttctg gtgaatgccg gtggtgagcc gtttgcggtg gttcaggtac
     2341 agcggcggtt tgcaccggaa gctgtcagcc attcactggc actggcggcg tcactggacg
     2401 cgcaggggta cagcgtcagt gacatcatcc atatcctgat ggcggaagga agtcaggcat
     2461 gagcgcacgt tcacaggcac tggttcctct cagcacagag caacaggccg catggcgggc
     2521 ggtggcggag accgaaaaac gccgccatca gggtaacacg ctggctgaat atccgtatgc
     2581 gggggcgttt ttccgctgtc tgaacggaag ccgcaggata tcgctgtctg acctgcgatt
     2641 tttcatgccg tccctgaccg cagaagaact gcacggcaac cgtcttcagt ggctgtatgc
     2701 catcgatgtg ctgattgaaa cacagggcga ggtttgcctc cttccccttc caggcgatgc
     2761 agcagaacgc ttgttcccgt cggtgcggtt tcgtgtcagg gaacgcagtc gccataaatc
     2821 cgcgctggtc atgcagaagt acagccgcca gcaggcgagg gaggcagagc agaaagcccg
     2881 tgcgtaccag gcgctggtcg ctcaggctga aatcgaactg gcattccatt cgccggaaac
     2941 agtcggaagc tggtatgcac gctggagtga ccgggtggca gagcatgacc tggaaaccct
     3001 gttctggcag tggggcgagc gttttccgtc actggccgga atggaacgct ggcagtggca
     3061 ggatatgcca ttctggcagg tcatcgcaga ggctggtatg gcggcaaggg tggtcggtca
     3121 tgctgttcgt gagatggagc ggtggatggt gccgaacaaa ctccgggagg cagcgtgatg
     3181 cggcaacaat cacacggatt tcccgtgaac ggtctgaatg agcggattat tttcagggaa
     3241 agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc
     3301 tgcctcatga cgtgaaggtg gtttgttgcc gtgttgtgtg gcagaaagaa gatagccccg
     3361 tagtaagtta attttcatta accaccacga ggcatcccta tgtctagtcc acatcaggat
     3421 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gaagttccct
     3481 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa
     3541 atcgctgtgc gagattcgtt acagagacgg acacagggag gtggcggctt tcatggctta
     3601 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag gactgatgct
     3661 ggtctgacta ctgaagcgcc tttataaagg ggctgctggt tcgccggtag cccctttctc
     3721 cttgctgatg ttgtacgggc atgaacaacc tgacttcagg aaggtcgttt cccttcagga
     3781 acggggatga acgcgcgcct gcggcgcccg ttcttttccc ccgccttctc tggttatggc
     3841 ctgtcagaaa tcacatcacc ttccctgtga ttattctctt tttcttcctg ctctgattct
     3901 gactactgca gtgttgtcct gtctgccggg cgtaccttcg gctcccggct gcctggcggc
     3961 atccggtcgt caggcccgac tccgacaggc agctgctggc gcacctgcca gtctccgccg
     4021 aacccgctgg cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaac ccctggcttg
     4081 cagcgaccgt tccggtcgcg ccgcctctgt cgcgccgtga cctgcgctcc cggcatcccg
     4141 gcaaaaggcg ccgggatgta aggcgcaacg ccgtttcgca aaccgcgctt cacgtcgttg
     4201 cggcgggtcc ctgcccgttg ttgtgtccgg tgccattcac cagtttaagc ccctgtcgcc
     4261 gggggcatag acagccacaa ccgtctccgc cagtccaggg gtgaaatgca catcccgcaa
     4321 aactttttgc ccagaagcag gcaaaaactt ttgcggtctg cccccggaca gtcgtttccg
     4381 gccgtggcag tgatgcccca tgcggcgaag gttctcaaac aggtgatggt gaataccgga
     4441 gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat
     4501 gatgggggct gaaaaccaga gagaggagaa agcaggatga gtacccgaaa tatccacgtt
     4561 aacaccgcgt cgtacacgct tcttgttgcc gggaagaaaa aaaacaccgg ggaagagtgg
     4621 gacgtcctgg aattcagcag cctgactgag ctgaaaaaat atcgcaaaag ccacccggaa
     4681 aagatggcct tcagttatag ctacgcgctc agtcagggtg tggataagca gttccgccat
     4741 atcaacattg cggaagccga tcacttcaaa cagttcctgc gtcagattaa gcgtgccggc
     4801 ctcgatatcc gggcgatctg ctgaccggat gtgatgaata tccctcctga ttgttgcaat
     4861 cttgttaatg gagatcgggg ggatgatatt aagcggaaaa attttttatt aaatttattc
     4921 tttcataaag gagtagctgt tatgcgatta gcttcccgtt ttggtcggta taattccatc
     4981 cgccgtgaac gtcctttaac ggatgatgaa ttaatgcagt tcgtgccttc ggtattttcc
     5041 ggtgataaac atgagtcccg gagtgaacgt tatacgtata ttccaacaat caatatcatc
     5101 aataagttac gtgatgaagg tttccagcca ttctttgcct gtcagagtcg gattcgtgat
     5161 ttgggacgtc gcgaatacag taaacatatg ttacgtcttc gcagggaagg gcatattaac
     5221 ggacaggaag ttcctgaaat tatcctgctt aattcacatg atggttcatc cagttatcag
     5281 atgatccccg gaatttttcg ttttgtctgc acaaatggcc tggtgtgcgg aaataatttt
     5341 ggcgaaatcc gcgttccaca taaaggtgat attgtcgggc aggttatcga gggagcgtat
     5401 gaagtgctcg gtgtctttga taaggtcact gataatatgg aggcgatgaa agaaattcat
     5461 cttaacagtg acgagcaaca tttatttggc agagctgcac tgatggtcag gtatgaagat
     5521 gaaaataaaa cgccagtgac acctgaacag ataattactc cccgtcgtcg ggaagacaaa
     5581 cagaacgatc tctggacaac ctggcagcgg gttcaggaga atatgataaa aggtggatta
     5641 tcggggcgaa gtgcctccgg gaaaaatacc aggacaagag ccattacagg tattgatggt
     5701 gatataagaa tcaacaaggc gttatgggtg attgccgaac agttcagaaa gtggaagtca
     5761 tgataatata agtaaagccc cgaaaaaatt ttcggggctt tacttatatt atcatttttc
     5821 ggcattgctg tgtcgtctga tatacctcaa tttcatcaac cggatttccg gttgtctgta
     5881 aatattgtgc ccgcgaaaaa tggtctggca ggaggctgca cagttaccgg gtatatataa
     5941 gatgggttcc cgcttcgcgg ggcttcggcc agtcagtatt gtcactcata tgatattttt
     6001 gtgtgtggcc ttccatgccg cttttgcggc ataaccggta tctgacaatg tctgtaagat
     6061 taattgttct gcacgctgtt aatttccgga agtgatttct tcgtggccgg aagccggata
     6121 cctttactgc tttttatccc agtataaatc cggtaaacat ctgatgcgta ggcaagacgt
     6181 ctctggttct ggcgttcggt tttcctgaat ccggcattgt atgcaccaac ggcctcccag
     6241 gtgacgcccc attttttaaa tgctattgcc agataataag caccggtata aatgttcatg
     6301 cagggatctg ttgtcagatg ttctggctta attccgtagc gggccagttc gttaaaatgc
     6361 tgggaatcta cctgcatcag tccgctgcca tatcccgtta ccggattaat accgatggca
     6421 ttaacccggt aacgggattc ttgccatgat attgctctca gtaaatcagg atctattttg
     6481 taatcccggc ctgcaagatc aaagcaatca gtggcctggc agatctcatt tataaacatc
     6541 aggcagatgg ctaacatcca ttttttcatt tttccacctc tggtgacttt atccgtaaat
     6601 aatttaaccc actccacaaa aaggctcaac aggttggtgg ttctcaccac caaaagcacc
     6661 acaccccacg caaaaacaag tttttgctga tttttcttta taaatagaga gttatgaaaa
     6721 attagtttct cttactctct ttatgata
//
LOCUS       DACAPB010000217         6748 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.218, whole genome shotgun sequence.
ACCESSION   DACAPB010000217 DACAPB010000000
VERSION     DACAPB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.218"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..2168)
                     /locus_tag="IT904_004155"
     CDS             complement(<1..2168)
                     /locus_tag="IT904_004155"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HAP3193851.1"
                     /translation="MAPISYKKQELSETDELDLGRLVGEFIDHRKLILSVTIFFTVIA
                     LLYAMFSTPIYQADALVQVEQKQGNALLDSLNQVLPTGTQQSAPEIALLQSRMVLGKT
                     VDDLNLEIAIEKDYFPVFGRGWARLTGTDGGNIKVSKLILKKNDIENVKLKVINENDF
                     LVTTSSGDIKGRVGTAIDNRDFTLKVDEINADEGSSFTIQKITRLQAINNLLKVFSVT
                     DQGKDTGMLNLTLVGEDPNQITNILNTISDNYLAQNISRQAAQDEKSLEFLKAQLPKV
                     RDELDDAENKLNAYRRKKDSVDLTMEAQAVLNQIVNVDNQLNELTFREAEISQLFTTD
                     HPTYKALLEKKKTLAQEKVRLNNQVTSMPTTQQEILRLSRDVESGRAVYMQLLNRQQE
                     LNISKSSTIGNVRIVDSAIAEPKPVKPKKIMVVLIGLVFGLFVSVGIVLARMFLRRGI
                     ESPEELEDMGINVYASVPVSEWLTQKHFKNGKRLAAKDVFKEDRESLLALENPADLAI
                     EAIRSLRTSLHFAMLESSNNILMISGASPNAGKSFISSNLGAIVAQTGKRVLFIDADM
                     RKGHIHKVFGVSNSNGISEILSGKSTVGSAILKTQLDNLDLITRGQVPPNPSELLMHE
                     RLKLFLESVSKEYDLILVDTPPILAVTDAAIIGQYVGTVLLVARFELNTAKEIEISKR
                     RFEQSGIPVKGCILNGVVKKASSYYGYGYNHYGYSYNTDK"
     gene            complement(2187..2618)
                     /locus_tag="IT904_004156"
     CDS             complement(2187..2618)
                     /locus_tag="IT904_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704361.1"
                     /note="Wzb shows phosphatase activity towards the
                     autophosphorylated Wzc protein, which induces colanic acid
                     biosynthesis; catalyzes the phosphorylation of UDP-glucose
                     dehydrogenase, an enzyme involved in colanic acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HAP3193852.1"
                     /translation="MFDSVLVVCTGNICRSPMGERILQSLLPEKIIKSAGVGALVGHP
                     ADPLALQVSNEKGINLEGHSGTQFTSAMSIEYDLILVMDKNHIEQVSKIAPHARGKTM
                     LIGRWIGNKEIPDPYKQSKEAFEFVYQLIDEACHSWAQRLR"
     gene            complement(2625..3758)
                     /locus_tag="IT904_004157"
     CDS             complement(2625..3758)
                     /locus_tag="IT904_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009484588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAP3193853.1"
                     /translation="MKKKLVRFSALALAIGFLSGCTIIPGQGLNSLRKNVVELPDSDY
                     DLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLLRNYEYRIGVGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNSDGTIFYPYIGRVQVAGKTLAQVRQDIASRLTTYIESPQ
                     VDVSVAAFRSQKVYVTGEVTKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHN
                     GKDTKISLYALMQKGDLSQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGM
                     TLAEAIGNAEGMSQAFSDATGIFVIRQLKNDKKGKIANIYQLNAQDASAMVLGTEFQL
                     QPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETTRYIKNW"
     gene            complement(3904..5337)
                     /locus_tag="IT904_004158"
     CDS             complement(3904..5337)
                     /locus_tag="IT904_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HAP3193854.1"
                     /translation="MIKITRIAVTLGLLSSLGAQAYAAGLVVNDNDLRNDLAWLSVRG
                     VIHLSLSTWPLSQEEIARALKKAKPSYSSEQAVLARINQRLAALKADFRVTGYTSTDQ
                     PGTPQGFGQTQPADNSLGLAFNNSGEWWDIHLQGNVEGGERISNGSRFNANGAYGAVK
                     FWNQWLSFGQVPQWWGPGYEGSLIRGDAMRPMTGFLMQRAEQAAPETWWLRWVGPWQY
                     QISASQMNQYTAVPHAKIIGGRFTFTPFQSLELGASRIMQWGGEGRPESFSSFWDGFT
                     GKDNTGTDNEPGNQLAGFDFKFKLEPTLGWPVSFYGQMIGEDEAGFLPSANTFLGGVE
                     GHHGWGKNAVNWYVEAHDTRSNMSRTNYIYTHHIYKGGYYQQGYPLGDAMGGDGQLIA
                     GRVELITEDNQRWSTRLVYAKVNPENQSINKAFPHADTLKGVQLGWSGDVYQSVRLNT
                     SLWYTNANNSDSDDVGASAGIEIPFSL"
     gene            5627..5749
                     /locus_tag="IT904_004159"
     CDS             5627..5749
                     /locus_tag="IT904_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193855.1"
                     /translation="MMIILTGSIFTAVEENLCAAASGFSDATALGLTATSALTT"
     gene            5918..6455
                     /locus_tag="IT904_004160"
                     /pseudo
     CDS             5918..6455
                     /locus_tag="IT904_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1824 a         1602 c         1339 g         1983 t
ORIGIN      
        1 tttttgtcag tattatatga atagccataa tggttataac cataaccgta ataactactt
       61 gcttttttta ccacaccatt gagtatgcaa ccttttactg gaataccact ctgttcaaat
      121 ctccgttttg aaatttcgat ctctttagct gtatttaatt caaatctagc cacaagtaat
      181 accgtaccta catattggcc gataatcgca gcatcagtaa cagccaatat tggcggggta
      241 tctactaaaa tcaagtcata ttctttagaa actgattcta aaaataattt caatctctca
      301 tgcattagta gttctgaagg atttggcgga acttgtcctc tagtaatgag atcaagatta
      361 tccaattgtg tttttaaaat cgcgctaccg actgtggatt tccctgacaa gatttctgat
      421 atgccattgc tgttacttac cccgaatact ttatgaatat gaccttttct catatcagca
      481 tcaatgaaga gtactcgctt tcctgtctga gcgacaattg cccccaaatt gctactaata
      541 aaagacttac ctgcatttgg gctagcccca gatatcatta atatattatt gctagattca
      601 agcattgcaa aatgtaagct agttcttaaa cttcttattg cttcaatagc aagatcagct
      661 ggattttcca atgcaagaag ggattcacga tcctctttga acacatcttt tgcagccaat
      721 cgttttccat ttttaaaatg cttctgcgtt aaccactccg aaactggaac acttgcataa
      781 acattaattc ccatatcttc gagttcttca ggagattcta ttccacggcg taagaacatt
      841 ctagctaaga ctatgccgac tgaaacaaac aacccaaaga ctaatccgat cagtacaacc
      901 attatttttt taggctttac tggttttggt tcagcaatag cagaatcaac gatcctaaca
      961 ttcccaattg tactggattt ggaaatattt aattcttgct gtctatttag caactgcata
     1021 tagacagcgc gaccggattc aacatctcta ctcaacctta aaatttcctg ttgagtcgtt
     1081 ggcatcgatg taacttggtt attcagccta actttttctt gtgctaaagt cttctttttt
     1141 tcaagcaatg ctttgtatgt tggatgatca gtcgtaaaca attgagaaat ttcagcttct
     1201 ctaaatgtaa gttcatttag ctgattatct acattaacga tttggtttaa aaccgcttga
     1261 gcttccattg ttaaatcaac ggagtctttt tttcgcctat aagcattcaa cttattttca
     1321 gcatcatcaa gctcgtcacg aaccttcggt aattgcgctt ttaaaaactc taaacttttt
     1381 tcatcttgcg cagcctggcg tgaaatgttt tgagccaaat aattatcact aattgtattt
     1441 aaaatattag taatctgatt tggatcttca ccaaccaaag ttaaatttag catcccagta
     1501 tctttaccct gatctgtgac actgaatact ttaagtaaat tgttaatggc ttgtaaccgt
     1561 gtgatctttt ggatcgtaaa gctggatcct tcatcagcgt ttatttcatc cacttttaaa
     1621 gtaaaatcac gattatcgat cgccgttccc actctgcctt tgatatcgcc ggaacttgtt
     1681 gttacaagaa aatcattttc attgataact ttaagtttta cattctctat atcatttttt
     1741 ttcagaatga gtttactgac tttaatattt ccaccatctg tacctgtcaa gcgagcccaa
     1801 ccgcggccaa atactggaaa ataatctttt tcaattgcta tttctaaatt caggtcatca
     1861 acagttttac ctaataccat tcgagattgc aataacgcaa tctccggtgc tgattgctga
     1921 gtacctgtag gtagcacctg atttaaacta tctaataaag catttccctg cttttgttcg
     1981 acttgaacta gagcatccgc ttgataaatc ggtgttgaga acattgcata gagaagtgca
     2041 atgactgtaa aaaaaatagt tacgctaagg ataagttttc gatgatcaat aaattctcca
     2101 actaaacgac ctaagtctaa ttcatctgtt tctgatagtt cttgcttctt ataacttata
     2161 ggtgccataa atatgtcccg ctttattcaa cgaagtctct gagcccagct atgacatgct
     2221 tcatctatta attgataaac aaattcaaaa gcttctttgc tttgcttata tggatctgga
     2281 atttctttat ttccaatcca acgcccaatt aacattgttt ttccgcgagc atgaggcgca
     2341 attttactaa cctgctcaat atgattctta tccattacca gtattagatc atattcaata
     2401 ctcatagcac ttgtgaactg cgttcctgaa tgtccttcaa gatttatgcc tttttcattt
     2461 gagacttgta aagcaagtgg atcagcagga tgtccaacca aggcacctac tcccgctgat
     2521 ttgattattt tttcgggtaa caaagactgt aatattctct cgcccatagg tgaacgacaa
     2581 atatttccag tacataccac taatacagag tcaaacatat gtcatcacca atttttaatg
     2641 tagcgtgtag tttcagtcat atcatgaaca cctgtaattg taggtactag ctgagatata
     2701 acgcgattcc atcttacaag tggagcagtg gtaacataga caatatcata gggctgtaac
     2761 tgaaactcag ttccaagtac catcgctgaa gcatcttgcg cattaagctg ataaatatta
     2821 gctattttac cttttttatc attttttagt tgtcttataa caaatatacc agtggcatca
     2881 ctaaaagctt gggacatacc ttcggcatta ccaattgctt ctgctaaagt catcccgctt
     2941 cgatccattt tcattgtact ttgtttacca acttctccca tgacaaatac ttttaaatca
     3001 tcattacgtg gaacaaatag tatatccccc ggatacagca aatgattttg tgacaagtcc
     3061 cctttttgca tcaacgcata taaagatatc ttcgtatcct taccgttatg tgttaacaca
     3121 acatttcgcc aatcagcatc tggagcaagc ccgccggccg cgttaatggc atccatcacc
     3181 gttaatggaa tattggttat gggctgctgt cctgatttag taacctctcc tgtcacatag
     3241 actttttgcg aacgaaacgc agcaacacta acatcaacct gtgggctttc aatataagtg
     3301 gttaaacggc tggctatatc ctgtcgtacc tgagcgagag ttttccccgc tacctgcacc
     3361 ctaccaatat aaggatagaa aatggtacca tcggaattaa cccagttacc ggtgtcgcta
     3421 gcgctacggt actggcctgc aggtgtcgtc agttccgggt gatcccatac cgtaaccatc
     3481 agtacgtcac caaccccaat gcggtattca taattgcgca gtaaattatc aagctgcggg
     3541 tttggacgag cgagtacagt ctccggacga agttgatcga tcagtcctgg ggtcatcgga
     3601 tacacgttaa ccagtttatc cagatcgtag tcactgtccg gaagctcaac cacgttcttg
     3661 cgcagactat ttaatccctg gccagggatg attgtacaac ccgataaaaa cccaattgcc
     3721 aatgctaatg ccgaaaatct aacaagtttt ttcttcataa tgtcacatca tcagtaaacc
     3781 aaaatttgcc aattcctgag cagctgtgtt agccattact tccctgcagg cttatggaga
     3841 gaaaaaaact gaccgcgtcc tgcagcgcta ccaccctttc tcccctattt tcagcattca
     3901 gtcttataaa ctaaatggta tttctatccc tgcgctggcc ccaacgtcat cgctgtcgct
     3961 gttgttagcg ttggtgtacc acagcgaggt gttcagacgt acagactgat aaacatcccc
     4021 gctccagcct agctgtacac ctttcaaggt atccgcatgg gggaatgctt tattgatcga
     4081 ctgattttcc gggttaactt tggcgtaaac caggcgcgta ctccagcgct gattatcttc
     4141 tgtaataagt tcaaccctac cggcaatgag ttgaccatcc ccgcccatcg cgtcacccaa
     4201 cggatatccc tgttggtaat aaccaccctt gtaaatatga tgcgtgtata tgtaattagt
     4261 acggctcata ttagatcttg tatcgtgagc ctcaacgtac cagtttaccg cattcttacc
     4321 ccaaccatga tgtccttcca ctccaccaag gaatgtattt gctgagggaa ggaaacctgc
     4381 ctcatcctcg ccgatcatct gtccgtaaaa actcaccggc cagccaaggg tcggttccag
     4441 tttgaattta aagtcaaacc cggccagctg gttacccggt tcgttatctg tcccggtatt
     4501 atcttttcca gtaaaaccat cccagaaact gctgaacgat tcagggcgtc cttccccacc
     4561 ccactgcata atgcgcgaag cacctaattc taatgactga aacggtgtaa aggtgaagcg
     4621 tccaccgata attttggcat ggggcaccgc agtatattga ttcatctggc tggcggagat
     4681 ctggtattgc catggcccca cccagcgcag ccaccaggtc tctggcgctg cctgctctgc
     4741 gcgctgcatc aggaaaccgg tcatcggtcg catggcatca ccgcggatca ggctaccttc
     4801 atagccaggc ccccaccact gtggtacctg accaaaagag agccactggt tccagaactt
     4861 caccgcgccg taggcaccgt tggcgttgaa gcgtgatccg ttgctgatcc gctctccccc
     4921 ttcaacgttg ccctggagat ggatatccca ccactcgccg ctgttgttga atgccaggcc
     4981 taacgagtta tccgctggct gtgtctgacc aaacccctgc ggagtacccg gctgatcggt
     5041 tgaagtgtag ccggtgaccc ggaaatccgc tttcaaggca gccagtcgct ggttaatacg
     5101 cgccagcacc gcttgctcag aagaatagga aggtttggcc tttttcagcg cccgggcgat
     5161 ctcttcctgg ctcagcggcc acgtcgacag gctcagatgg atgaccccgc gaacggaaag
     5221 ccaggccagg tcgttacgca agtcgttgtc atttaccact aacccggccg cgtaagcctg
     5281 ggctcccagc gaggaaagca aacccaacgt cacggcaatg cgcgtaattt ttatcattgt
     5341 acaagatcca ttttcagccc tgctgtcgta tctcaatgag aatgagggga ataccaagat
     5401 agaaagcgct cgcggcacca gctgacttcg ctgtgcaaca ccggcgaggc aagtacattt
     5461 accctaagct gcttgttagc cttataacat ccactcagct cacaatgtag acgaatttac
     5521 cctacaggct atcaatgaga catgaaagtt aatgtctagg cgatatcagc agccgctcct
     5581 aaacaacttc tgaggaaatc catagaaaat tgatagatat ctattgatga tgatcatcct
     5641 gaccgggtcg atatttacag ccgttgaaga gaacctctgt gcagcagcaa gcggcttcag
     5701 cgacgctacc gcccttggct taacagctac cagcgcactg actacctgaa cgacttaatt
     5761 ttttatcgac ctgtggatga ataattggtc aggtcattat ttttggcagc gctaagttta
     5821 cgggatttcg gtcacaattg ggagtgttgt tactgtagga taattcagga cacctcgaaa
     5881 tcctgttaat gttaaaacag tgaaaatgag gtgatgcatg atcaaaactc gtcggactaa
     5941 acgtaccttt tccgcggagt tcaagcttga agctttcgag caggtggtgg ttaaatacca
     6001 gcgtgatgtc agagaagtcg cgctggcact tgagctcaac cctgaccatt tgcgtaaatg
     6061 gatacggttg tataagcagg aacttcaggg tattgagccc gctggtaatg ctattagccc
     6121 tgaacaacgc gaaattcagc agcttaaagc gcagataaag tgccttgaga tggaaaaaga
     6181 aatactaaag caggctgccg tgctgatgag cgaaatcccc gggaagctgt cgcgctaatc
     6241 acacggctga aagcaaaatg gccagtgcga gttctttgtc atttgttcgg tatttaccgt
     6301 agcgtttatt acgcgcaggt gaagcgtcct gttaatgtgc aaagaattga gttacgaagt
     6361 cgggtgaggc tttccatgct ctcagtcgtg gcgcagccgg tagccgggca atcagtcaga
     6421 tgttgcgcca gagcggcgtt gatgcaggcc ggtggcgact gctgcttttt gcagccctgc
     6481 ggagaaacga gcgctgtgct gcaaaacggt gctatcgcca aaaaatgaga tgagttgcca
     6541 gttcatatac ctgtgatcct tcgatgatgt ccactgccgt gctcagggat tatccgtaat
     6601 gtctgtacca gctcacatgc caattcattt tatattttta tgcatgcaag tgagatgttt
     6661 aaagcggaaa catgatcgca gtcaagtttt atttttggct atcgttgact gaggtagcct
     6721 gagtttaacg gacactcctt cctgaaat
//
LOCUS       DACAPB010000218         6715 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.219, whole genome shotgun sequence.
ACCESSION   DACAPB010000218 DACAPB010000000
VERSION     DACAPB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.219"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..333
                     /locus_tag="IT904_004161"
     CDS             <1..333
                     /locus_tag="IT904_004161"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HAP3193856.1"
                     /translation="ISVLTDPTMGGVSASFAMLGDLNIAEPKALIGFAGPRVIEQTVR
                     EKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAKLMNLPAPNPEAPREGVVVPP
                     VPDQEPEA"
     gene            403..1671
                     /gene="folC"
                     /locus_tag="IT904_004162"
     CDS             403..1671
                     /gene="folC"
                     /locus_tag="IT904_004162"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP3193857.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            1661..2323
                     /gene="dedD"
                     /locus_tag="IT904_004163"
     CDS             1661..2323
                     /gene="dedD"
                     /locus_tag="IT904_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAP3193858.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            2582..3070
                     /gene="cvpA"
                     /locus_tag="IT904_004164"
     CDS             2582..3070
                     /gene="cvpA"
                     /locus_tag="IT904_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAP3193859.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            3107..4624
                     /gene="purF"
                     /locus_tag="IT904_004165"
     CDS             3107..4624
                     /gene="purF"
                     /locus_tag="IT904_004165"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP3193860.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLNEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            4719..5288
                     /locus_tag="IT904_004166"
     CDS             4719..5288
                     /locus_tag="IT904_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP3193861.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            5554..6336
                     /gene="argT"
                     /locus_tag="IT904_004167"
     CDS             5554..6336
                     /gene="argT"
                     /locus_tag="IT904_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAP3193862.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
BASE COUNT         1549 a         1683 c         1851 g         1632 t
ORIGIN      
        1 atctccgtgc tgaccgaccc gacgatgggc ggtgtttctg caagtttcgc catgctgggc
       61 gatctcaaca tcgctgaacc gaaagcgtta atcggctttg ccggtccgcg tgttatcgaa
      121 cagaccgttc gcgaaaaact gccgcctgga ttccagcgca gtgaattcct gatcgagaaa
      181 ggcgcgatcg acatgatcgt ccgtcgtccg gaaatgcgcc tgaaactggc gagcattctg
      241 gcgaagttga tgaatctgcc agcgccgaat cctgaagcgc cgcgtgaagg cgtagtggta
      301 cccccggtac cggatcagga acctgaggcc tgataactga taagggcagg gccactggct
      361 ctgccctttt gctattctca ccgtaacgaa tcagcggata ccatgattat caaacgcact
      421 cctcaagccg cgtcgcctct ggcttcgtgg ctttcttatc tggaaaacct gcacagtaaa
      481 actatcgatc tcggccttga gcgcgtgagc ctggtcgcgg cgcgtcttgg cgtcctgaaa
      541 ccagcgccat ttgtgtttac cgttgcgggt acgaatggca aaggcaccac ctgccgtacg
      601 ctggagtcga ttctgatggc ggcagggtac aaagtgggcg tctacagttc gcctcatctg
      661 gtgcgttata ccgagcgcgt acgtgtgcag ggccaggaat tgccggaatc ggcccacacc
      721 gcctcttttg cggagattga atcggcacgc ggtgatattt ccctgaccta tttcgagtac
      781 ggtacgctgt cggcgttgtg gctgttcaag caggcacaac ttgacgtggt gattctggaa
      841 gtagggctgg gcggtcgtct ggacgcaacc aatattgtcg acgccgatgt cgcggtagta
      901 accagtattg cgctggatca taccgactgg ctgggtccag atcgcgaaag tattggtcgc
      961 gagaaagcag gcatcttccg cagcgaaaaa ccggcaattg tcggtgagcc ggaaatgcct
     1021 tctaccattg ctgatgtggc gcaggaaaaa ggtgcactgt tacaacgtcg gggcgttgag
     1081 tggaactatt ccgtcaccga tcatgactgg gcgtttagcg atgctcacgg cacgctggaa
     1141 aatctgccgt tgccgcttgt cccgcaaccg aatgccgcaa cagcgctggc ggcactgcgt
     1201 gccagcgggc tggaagtcag tgaaaatgcc attcgcgacg ggattgccag cgcaattttg
     1261 ccgggacgtt tccagattgt gagcgagtcg ccacgcgtta tttttgatgt cgcgcataat
     1321 ccacatgcgg cggaatatct caccgggcgt atgaaagcgc taccgaaaaa cgggcgcgtg
     1381 ctggcggtta tcggtatgct acatgataaa gatattgccg gaactctggc ctggttgaaa
     1441 agcgtggttg atgactggta ttgtgcgcca ctggaagggc cgcgcggtgc cacggcagaa
     1501 caactgcttg agcatttggg taacggcaaa tcatttgata gcgttgcgca ggcatgggat
     1561 gccgcaatgg cggacgctaa agcggaagac accgtgctgg tgtgtggttc tttccacacg
     1621 gtcgcacatg tcatggaagt gattgacgcg aggagaagcg gtggcaagta agtttcagaa
     1681 tcggttagtg ggcacgatcg tgctggtggc gctgggggtg attgtacttc cagggctgct
     1741 ggacgggcag aaaaaacatt atcaggatga gttcgcggct atcccgctgg tgccgaaagc
     1801 gggcgatcgt gatgagcctg atatgatgcc agccgccacc caggcgttac cgacgcagcc
     1861 gccggaaggc gcagcggaag aggtgcgggc aggtgatgcc gcagcaccgt cgctcgatcc
     1921 ggccactatt gcagccaata acaccgagtt tgaaccggaa cctgcaccgg tcgccccacc
     1981 gaagccgaaa ccggtggagc cgcctaaacc aaaggttgaa gcaccacctg cgccgaagcc
     2041 agaaccgaag ccggtcgtgg aagaaaaagc tgcaccgacg ggtaaagcct atgttgtgca
     2101 actgggtgcg ctgaaaaatg ccgataaagt gaatgagatt gtcggtaagc tgcgcggtgc
     2161 cggttatcgg gtttatacgt cgccatccac gccagtgcag ggtaaaatta cccgtattct
     2221 ggttgggccg gatgcctcga aagataagct gaaaggttcg ctgggtgagt tgaagcaact
     2281 ttctggctta agtggcgtgg taatgggcta tacgccgaat taatacggtc ttgcctgatg
     2341 cgacgctggc gcgtcttatc aggcctacgc aggggtagaa ccgtaggtcg gataaggcgt
     2401 ttacgccgca tccgacacgc attgcccgat gccgcaaagg cataaaaagt cgatggcgtt
     2461 gaatattttt tcagcgccat ttttattgat gcgcgggaag gaaatcccta cgcaaacgtt
     2521 ttctttttct gttagaatgc gccccgaaca ggatgacagg gcgtaaaatc gtgggacaca
     2581 tatggtctgg attgattacg ccataatcgc ggtgattgct ttttcctctc tggttagcct
     2641 gatccgcggc tttgttcgtg aagcgttatc gctggtgaca tggggttgtg ctttctttgt
     2701 tgccagtcat tactacactt acctgtcagt ctggtttacg ggctttgaag acgaactggt
     2761 tcgaaatggg attgccatcg cggtactgtt tatcgctacc ctgatcgttg gtgctatcgt
     2821 gaacttcgtg ataggccagt tggtggagaa aacggggttg tcaggcaccg atcgggtgct
     2881 gggcgtctgt ttcggtgcgt tgcgcggtgt gttgattgtt gctgccattc tcttctttct
     2941 cgactccttt accggggtgt cgaaaagcga agactggagc aaatcacagc tgatcccgca
     3001 attcagtttt atcatcagat ggttttttga ttatctgcaa agctcgtcaa gtttcttgcc
     3061 cagagcgtaa gtgctctgag atgtggctta acgaggaaaa agacgtatgt gcggtattgt
     3121 cggtatcgcc ggtgttatgc cggttaacca gtcgatttat gatgccttaa cggtgcttca
     3181 gcatcgcggt caggatgccg ccggcatcat caccatagat gccaataact gcttccgttt
     3241 gcgtaaagcg aacgggctgg tgagcgatgt atttgaagct cgccatatgc agcgtttgca
     3301 gggcaatatg ggcattggtc atgtgcgtta ccccacggct ggcagctcca gcgcctctga
     3361 agcgcagccg ttttacgtta actccccgta tggcattacg cttgcccaca acggcaatct
     3421 gaccaacgct cacgagttgc gtaaaaaact gtttgaagaa aaacgccgcc acatcaacac
     3481 cacttccgac tcggaaattc tgcttaatat cttcgccagc gagctggaca atttccgcca
     3541 ctacccgctg gaagccgaca atattttcgc tgccattgct gccacaaacc gcttaatccg
     3601 cggcgcgtat gcctgtgtgg cgatgattat cggccacggt atggttgctt tccgcgatcc
     3661 aaacgggatt cgtccgctgg tactgggaaa acgtgatatt gacgagaacc gtacagaata
     3721 tatggtcgct tccgaaagcg tagcgctcga tacgctgggc tttgatttcc tgcgtgacgt
     3781 cgcgccgggc gaagcgattt acatcactga agaagggcag ttgtttaccc gtcaatgtgc
     3841 tgacaatccg gtcagcaatc cgtgcctgtt tgagtatgta tactttgctc gcccggactc
     3901 gttcatcgac aaaatttccg tttacagcgc gcgtgtgaat atgggcacga aactgggcga
     3961 gaaaattgcc cgcgaatggg aagatctgga tatcgacgtg gtgatcccga tcccagaaac
     4021 ctcgtgtgat atcgcgctgg aaattgctcg tattctgggc aaaccgtacc gccagggctt
     4081 cgttaaaaac cgctatgttg gccgcacctt tatcatgccg ggccagcagc tgcgtcgtaa
     4141 gtccgtgcgc cgtaaactga atgccaaccg cgccgagttc cgcgataaaa acgtcctgct
     4201 ggtcgacgac tccatcgtcc gtggcaccac ttctgagcag attatcgaga tggcacgcga
     4261 agccggagcg aagaaagtgt acctcgcttc tgcggcaccg gaaattcgct tcccgaacgt
     4321 ttatggtatt gatatgccga gcgccacgga gctaatcgct cacggtcgtg aagtggatga
     4381 aattcgccag atcatcggtg ctgacgggtt gattttccag gatctgaacg atctgaacga
     4441 agccgtccgc gcggaaaacc cggatattca gcagtttgaa tgctcggtat tcaacggcgt
     4501 ctacgtcacc aaagacgttg atcagggcta cctcgatttc ctcgatacgt tacgtaatga
     4561 tgacgccaaa gcagtgcaac gtcagaacga agtggaaaat ctcgaaatgc ataacgaagg
     4621 atgatgcctt cgctgagggt gccggtctgg caccctgact tgcaactccc gccgaaatcc
     4681 tgcaaagtct gcctgcaagt ctgacagggc aactatttat gaaacgactc attgtaggca
     4741 tcagcggtgc cagcggcgcg atttatggcg tgcgcttatt acaggttctg cgcgatgtca
     4801 cagatatcga aacgcatctg gtgatgagcc aggcagcgcg ccagacctta tccctcgaaa
     4861 cggatttttc tctgcgcgaa gtgcaggcat tagccgatgt cacgcacgat gcgcgcgata
     4921 ttgccgccag catctcttcc ggttctttcc agacgctggg gatggtgatt ttaccctgtt
     4981 caatcaaaac cctttccggc attgtccata gctatactga tggcttactg acccgtgcgg
     5041 cagatgtggt gctgaaagag cgtcgcccgt tggtgctctg cgtgcgtgaa acaccattgc
     5101 acttaggcca tctgcgttta atgactcagg cggcagaaat cggtgcggtg attatgcctc
     5161 ccgttccggc gttttatcat cgcccgcaat cccttgatga tgtgataaat cagacggtta
     5221 atcgtgttct tgaccagttt gcgataaccc ttcctgaaga tctctttgcc cgctggcagg
     5281 gcgcataact ccgctgttgc cctgtttcag ggcaattttg caaccgcgat caaatcctcg
     5341 acattttgtt tctgccattc aatcgaaacg ctgcgattca accgctatac ctgctatctt
     5401 caacttcagg acaataatgc aacgtcttat taacatattt aacgttgaat gttactgttg
     5461 tcgtcaagat ggcataagac ctgcatgaaa gagcctgcaa acacacaaca caatacacaa
     5521 cataaaaaag ccattttcac ttgagggtta tgtatgaaga agtcgattct cgctctgtct
     5581 ttgttagtcg gtctctccac agcggcttcc agctatgcgg cgctaccgga gacggtacgt
     5641 atcggaaccg ataccaccta cgcaccgttc tcatcgaaag atgctaaagg tgattttgtt
     5701 ggctttgata tcgatctcgg taacgagatg tgcaaacgga tgcaggtgaa atgtacctgg
     5761 gttgccagtg actttgacgc gctgatcccc tcactgaaag cgaaaaaaat cgacgctatt
     5821 atttcgtcgc tttccattac cgataaacgt cagcaggaga ttgccttctc cgacaagctg
     5881 tacgccgcag attctcgttt gattgcggcc aaaggttcac cgattcagcc aacgctggat
     5941 tcactgaaag gtaaacatgt tggtgtgctg cagggatcaa cccaggaagc ttacgctaac
     6001 gagacctggc gtagtaaagg cgtggatgtg gtggcctatg ccaaccagga tttggtctat
     6061 tccgatctgg ctgcaggacg tctggatgct gcgttacaag atgaagttgc tgccagcgaa
     6121 ggattcctca agcaacctgc tggtaaagat ttcgcctttg ctggctcatc agtaaaagac
     6181 aaaaaatact tcggtgatgg caccggtgta gggctacgta aagatgatgc tgaactgacg
     6241 gctgccttca ataaggcgct tggcgagctg cgtcaggacg gcacctacga caagatggcg
     6301 aaaaagtatt tcgactttaa tgtctacggt gactgatacg tcgctgggaa gctgtacctg
     6361 atggaatgat catcatggtg cacgccaggt ttgttgcact atcgtggtgc attgaaatgc
     6421 atacttaagc atttttaatg aaaaataata cgtctaacgg ggcgggatat tttgccttga
     6481 tggtcaattt tatggcacga taagtgtaac aaacctgtaa atattcccta taaaaagact
     6541 gtcagttgag gacattatga aaaaactggt gctatcgctc tctctggttc tggccttctc
     6601 cagcgcaact gcggcatttg ctgcgattcc gcaaaacatc cgcatcggta ccgacccgac
     6661 ctatgcgcca tttgaatcaa aaaattcaca aggcgaactg gttggcttcg atatc
//
LOCUS       DACAPB010000219         6631 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.220, whole genome shotgun sequence.
ACCESSION   DACAPB010000219 DACAPB010000000
VERSION     DACAPB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.220"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1392
                     /gene="espX1"
                     /locus_tag="IT904_004168"
     CDS             <1..1392
                     /gene="espX1"
                     /locus_tag="IT904_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HAP3193863.1"
                     /translation="CLYKSNIVDDVIINKTRQNELVKVFCEYKTEFLILFDDFFRSYN
                     LPKPSPVLHHFFQYTHLRDAYFYRCKLIEHTVQFYFFKHKGITLLRLDVFDDRTSECL
                     SEEIKIYQECHEKFVKFLKANFNHEIYPQLYTPEIFYEACRNLQSFYAHQETSQNAKY
                     SAPDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYS
                     QLCKKLSLFKSIPSRLIEKSAYCSTENIITNKFDVVFSYCGDNVKEFILLLPYNKSLE
                     MHELNEQNIQYLTALNINIHKMLLSNIIIEKSNLSYGYYFSCVLSNISCFESDLSNTI
                     FSNGEINNLFIKKSNIFGTSFTNTRIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKT
                     LASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLKSIN
                     ECMFLMKNIAHQN"
     gene            1626..2978
                     /locus_tag="IT904_004169"
                     /pseudo
     CDS             1626..2978
                     /locus_tag="IT904_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2991..3950
                     /locus_tag="IT904_004170"
     CDS             2991..3950
                     /locus_tag="IT904_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAP3193864.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNAFRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTANIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVVYN"
     gene            complement(3988..4887)
                     /gene="nhaR"
                     /locus_tag="IT904_004171"
     CDS             complement(3988..4887)
                     /gene="nhaR"
                     /locus_tag="IT904_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAP3193865.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(4953..6119)
                     /gene="nhaA"
                     /locus_tag="IT904_004172"
     CDS             complement(4953..6119)
                     /gene="nhaA"
                     /locus_tag="IT904_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAP3193866.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         2173 a         1390 c         1277 g         1791 t
ORIGIN      
        1 tgtctttata aatcaaatat agtagatgat gtaataataa ataaaacaag acaaaatgag
       61 ttagtaaaag tattttgcga gtataaaaca gagttcttaa ttttgtttga cgactttttt
      121 agaagctata acttgcctaa accatctcca gtccttcatc atttttttca atatactcat
      181 ctgagagatg catattttta tagatgtaaa ctcatagaac atacagttca attttatttt
      241 tttaaacaca aaggcatcac attacttaga ttggatgttt ttgatgacag aacaagcgaa
      301 tgtctttctg aagagattaa gatatatcaa gaatgccatg aaaaattcgt taagttttta
      361 aaggcaaact tcaaccatga aatataccca cagctatata ctcctgaaat attttatgag
      421 gcctgcagaa atctccagtc attttatgct catcaggaga catcacaaaa cgctaaatat
      481 agtgcaccag acaagaaaaa aagtcatctt aaccaggaaa tcaaaaagct gattaaaaaa
      541 aatattttcc ctgagctata caatgagcaa tgcaataaaa tcccctcctc atcgatagat
      601 gataaccagg gaataatgtg gcagaacttt aaaactagca atattgcata ttcacaacta
      661 tgtaaaaaat tatctttatt taaaagcata cccagtcgat taatagaaaa atcagcttat
      721 tgctcaactg aaaacataat caccaataaa tttgatgttg tcttttctta ctgcggagat
      781 aatgtaaagg aatttatact tttattaccc tataataaat ctctggagat gcatgaactt
      841 aacgagcaaa acatccagta tttgactgcc cttaatatta acatacataa aatgttgcta
      901 tcaaatataa taatagaaaa gagcaacctt tcatatgggt attattttag ttgtgtatta
      961 tccaatatat catgttttga aagcgactta tcaaacacca tcttttcaaa tggagaaata
     1021 aacaacctct tcattaaaaa aagcaacata tttggaacat cgttcactaa taccaggata
     1081 aaaaatttgc gctgtgaaga tattatgcct ggccgatgga ccactcaact tgtcaataag
     1141 cacttaggtt acagatacac aggagtattc aaaaccctgg caagtattga tgacaaaccg
     1201 tcccgtttcg aaattttgat accattggta caaactttag ttcgcgacaa tgtaaagcta
     1261 aataatgatg tgtataaaga actgaaaaag tttatgcacg attacgacaa aacatcccca
     1321 gaaatgagaa aataccttaa aagcatcaac gagtgtatgt tccttatgaa aaacatagct
     1381 catcaaaatt aaaaaatctc aaaaaagaag taatttcaga atgaaattaa ctgtgatgat
     1441 tattaattca ctacagagga ataaaaaata atctgaagca tgctctgaaa catcctactc
     1501 tatcattcta atgatagaaa cttgctcttc ttataaagaa gggaacaatt aagaatatta
     1561 aattttcaaa ctttcatcaa cttcacatca agaaattaaa aaacttaaac attaatctaa
     1621 ataataacaa cctgatcaca taatcgggta cgattttatc cgataactat aatgctataa
     1681 ctctgggaaa tggttggaat acaccgctgg gtgccatctc ttttgacgct acccgatcga
     1741 gtagtaagtt aaataatgat accagacacg aaggtacgag ctatcaggtc gcctataata
     1801 aatatctatt gcaaacggcc actcacttta gcgtggccgc ctggcgttat gcttcgcagg
     1861 attataggac attcagcgac catctttacg aaaatgataa aataaatcat cagagtgact
     1921 atgacgattt ttataatatt ggcagaaaaa atagcctttc tgccaatatt atgcaacctt
     1981 tatctaataa tctgggaaat gtatcattaa gtgcgctttg gcggaattac tgggggcgaa
     2041 gtggaaatgc taaagattac caattcagtt attccaatag ctggcaacgc atcagttata
     2101 ctttctctgc cagccaatcc tatgatgaaa atgacaaaga agaggagcgt tttaatctgt
     2161 ttatctccat tcctttctac tggggggatg atattgccaa aacacgtcaa caaattaact
     2221 tatcgaattc gacctcattt tccaaagatg gctattcctc caacaatact ggaattactg
     2281 gcatagccgg tgaacatgat cagttaaatt atggtatata tgttaatcag caacaacaaa
     2341 ataatgatac atcgcttggt acgaatttaa gctggagaac tcccatcgcc ataatagatg
     2401 gcagctatag tcattctaaa aacgcctggc aaagtggtgg aagtattagt agtggattag
     2461 ttgtctggtc cggtggtatt aatatcacta accagttatc cgatacattt gcaattctgg
     2521 atgagcctgg attagaaggc gcgcatatta atggacaaaa atacaaccga acaaacagca
     2581 aaggccaggt tgtttacgac ccgattatac ctcatcgtga aaaccatctg gtacttgata
     2641 tagcaaacag tgaaagtgaa acagaattgc agggcaatcg tcaaattatt gcgccttacc
     2701 gtggagcagt ttcttatgtg cagtttacaa ctgaccaacg taagccctgg tatatacagg
     2761 cactgcgtcc cgatggttcg ccattaacct ttggctatga cgtactggat ctccaggaaa
     2821 acaatattgg agtcgttggc cagggtagtc gcctttttat tcgcgtagat gaaattccaa
     2881 ctggcataaa agttgctctc aatgatgaac agaatttatt ctgtactatt acttttcaac
     2941 acgttatcga tgaaaacaaa acatatatat gccagtaaaa ggagtttact atgaagtggc
     3001 ttttattaat caccttgagt ctatattctt ttattgtgca aagtgctccc tgtggattga
     3061 ccaatgtcgg ggaacaacgt ggaacatata tactcaagcc actgagtatg aaaggtaatc
     3121 tcaccgccga aaaaattgaa aacaccggaa atatcattga gtgtgcagac ttacaagaga
     3181 cgaccaacgc cattaaagtt cattttttaa cacagaacgc ttttaggttt aaagtcggaa
     3241 gtcgtcatta tattattaat ttcccctttg aatctgacgg taaaaaaata gagcttccag
     3301 gaaggtcatc ttataattta gaagaaatgc tatctgcgat aaatttcacc attccatact
     3361 ctatcgcatc tgtatcagat agttccaaca ccgcaaatat ttttccagga actccttttc
     3421 ctttgttgtt cgaaattgaa gtgagttcat gtacagggaa taatactaat tgtaatagcg
     3481 tccgctttaa ttataattta aatactattt tacaggtaaa tttaatgacc tgtggttttg
     3541 aagatcaatc catcgacact ggacgatttg tactgtctcg tatcaataat gaaaattatc
     3601 agtttcatca tataagattt gactgcatcc agggcgagca aggtgaattg gtttttgaac
     3661 ccagtgatgt tgaatattac tttgaaccag tgacgataca ggagtcagga accagcatat
     3721 taaaaaatga gttagaaaac agtgaggacg gtgcgggtca agtaggcttt caactctcca
     3781 atgatggtac gaatgaaatc cagtatggta aaagcaatta ctactctttt cagcatcccc
     3841 atgaaggaag taatcagatt cctctattta ttcgcccgcg tacatatgga aataatgtct
     3901 caagcgggca aataatgagc cgagtcaaaa tcgtggttgt gtacaactaa tctatctcct
     3961 ccactttaac tttgggggag ctgccgatta acgcgccgct ggactaaaaa gcgcagaata
     4021 atccgtattg cagattcgct gtaccgccgg gtgctgaatc atccgctcag caaaaatagc
     4081 atggtactct tccatcacat tctcgacgcg accaatttct acgacagttt tatcggcata
     4141 aaagtcatat gcataaagcg ttggggcaac gaagattgca ttgtgcattg caccaaaagc
     4201 tttcatcaac gcggcatcat caaactcgcc gaggatttct acgtttaatc cctgggagtt
     4261 aaaccagtta agcaatttgc gccctaacat tgaacgtcgc ccaggaatca aaagtcgccg
     4321 ttcttccaga caagccggga acggtttttc tggtggtgga tttgtacacc agaaactcac
     4381 gccacattcg ccaattctca cggagaacag gccttcctgc tgcgtagagt ctatcggaca
     4441 gtcggaaatg atcatatcca gtttatgctg gcttaattgc tccagtagca tttcgtgggt
     4501 ggattcgaag cagcgaagat gaatcggctc gccttctacc actgcagcat tcagcacgct
     4561 actgaccagg cgtttggaaa gtgcatcagc cacgccaacg tcaaacaata aattggattc
     4621 tttgcgatag ttcacaatat ccagcatttc ctggcttaag gtgaacattt tatcggcata
     4681 gcgatagacc agttctccca gctcgctggg ttcgagacca cgtcccttgc gcttaaataa
     4741 tttgccttgc aggcgctctt ccagagcccg gatctgtccg gtaatggttt gtggagttaa
     4801 ataaagcgcc tccgctgcgc caaccacgga accttcttta tagacatgcc agaagtaata
     4861 caagtggttg taattgatat gagacatgct catttctctc cctgataaca atgaaaaggg
     4921 agccgtttat ggctccccgg taaaccgtcc tgtcaaactg atggacgcaa acgaacgcgt
     4981 aaccagctgt atccaattac cgccgaagag atagaaccga ccaggatacc gagtttcgcc
     5041 cagttaatca gttctggatc tacgctacca aaggccaggc tggcaataaa gatagacata
     5101 gtaaaaccga taccgcacag gatccccacc gccataattt gctgataagt cgttccctca
     5161 ggcagatgcg ccagtttcaa acgcagcgcc aaccagcaga acagactaat ccccagcggt
     5221 ttgccaatca gcaagccagc gatgatcccc aatggcagaa tggaggtcaa gccatccagc
     5281 gtgacacctt gcagtgaaac gccagcatta gcaaatgcaa acagcggcaa aatcagatac
     5341 gccacccacg ggtgtaacac atgctccagt cgcttcgctg gagaacgccc atgcttctct
     5401 ttcaaaggaa taaagaagcc gacaattacc cccgccaggg ttgcgtgaac ccccgatttc
     5461 aacaccgcag tccacaacac tacgccaacc agaatataga cgcccgtgcg gcgtacacca
     5521 cacagattca ataccgcgag taccgcaatt gctacagccg cgacgccaag agaggccatc
     5581 gataagtcat tagtgtagaa caatgcgatg ataatgatgg ccccaagatc gtcgataata
     5641 gccagagcca tcaaaaagat cttcagcgct aacggaacac gacttcccaa cagcgccagc
     5701 acaccaagtg caaaggcaat gtcagtagcc gccgggatcg cccacccttc gcgggtaatc
     5761 ggatcggcat agttaaaggc cagatagagt aatgccggaa caatcatccc accaatagcg
     5821 gcgataactg gaaatgcggc ctggcgtaag ctggctagcg atccttgcat cagttcacgt
     5881 ttaacttcca gaccgactaa caggaaaaat accgccatca gcgcgtcatt tatccataac
     5941 agcatgtttt tgttgatttc gagtgaacca acccggagct gaaccggcgt ctccagaaag
     6001 tcgtgatacc atccactggt tgcgccgcta ttggccataa tcatcgccag gatagcggca
     6061 atgataagaa taatgcctcc cgaggcatca ctgctaaaga atcgatgcag atgtttcact
     6121 ttttatttct ctttcaggtg aatagatcgt ttttactatt ttaccccgca caaatcatcg
     6181 ttaaaatcag attatagtgt aggaataact cgttttttac gatccattga agaatttgcg
     6241 ccatcagcgg tgagatgagt ttttgacggc tgtaagcgct cacgagtgac cctaagccca
     6301 gataatcata gcttaaggtg aggatttctg agggattaat taggaaaagt aataattaat
     6361 ttcttgtcag tcggcgttat ttgttaggac aatatttcaa aaataatcca gcttgtcgtc
     6421 aataattgcg ataaagagat aaattaaagt taaataatta aatcattaaa aaattacgcc
     6481 acaaaaatgc gaggcgtctt caggattaga atcgataaaa caaaatgggt taaagagagc
     6541 gatataatag cggcgggtgc ttgaggctgt ctgtctcagg cattagctga acggcagata
     6601 gagaaaagcc ccgagtgata ttttaccatc a
//
LOCUS       DACAPB010000220         6431 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.221, whole genome shotgun sequence.
ACCESSION   DACAPB010000220 DACAPB010000000
VERSION     DACAPB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.221"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(294..1727)
                     /locus_tag="IT904_004173"
     CDS             complement(294..1727)
                     /locus_tag="IT904_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3193867.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(1790..2239)
                     /locus_tag="IT904_004174"
     CDS             complement(1790..2239)
                     /locus_tag="IT904_004174"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAP3193868.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(2236..5328)
                     /gene="ebgA"
                     /locus_tag="IT904_004175"
     CDS             complement(2236..5328)
                     /gene="ebgA"
                     /locus_tag="IT904_004175"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAP3193869.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYPQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(5512..6414)
                     /gene="ebgR"
                     /locus_tag="IT904_004176"
     CDS             complement(5512..6414)
                     /gene="ebgR"
                     /locus_tag="IT904_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAP3193870.1"
                     /translation="MNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSY
                     QQELEINDPYYLAIRHGIETQCEKLGIELTNCYEHSGLPDIKNVTGILIVGKPTPALR
                     AAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFIGGEDEPG
                     KADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFVASDSI
                     AIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEK
                     ARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1575 a         1607 c         1701 g         1548 t
ORIGIN      
        1 attcgccgta aaaacgaaat gaaggagaag atgtcgaggt aattgcaggt gtttttgtgt
       61 cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga aaaggcgtag caaaacggca
      121 gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat tgtgttaatc catgagctaa
      181 agaaaaagta cagcctttta tcgttgtaat tataaaaata taaaggcaga aactattttc
      241 cctctcctca tccaggaaga ggaaaaatgc ctgctcattt tttattattc tgcttaatca
      301 acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag ccgttaatcc ctttatatat
      361 ttactgtatt tccaccacgc aaagccaagg aagataacaa taccgccgac gttataaaaa
      421 atgatggtca gaatattcgc gccagtcggg aatgtcgaag cgacaaaccc tacggcaaat
      481 atcgcaatca gcattgaaac aacgataata ccggtgcggc gggagcccat acggaaatcg
      541 cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag ccagcatgat gaataacggc
      601 ggaagcatgg acgctgcggc ggtcatatta ataatagtat tcatcagatc ctgcacggta
      661 ttggaaccga gcatcgggat aatcatcagc gggatgacga tcaggaactg gatccacgct
      721 gcgcgcgccg gaacgccgtt ttcgttcagt tcgacggttt tcttaccaaa gatgccttcc
      781 gggatttcgg agaagaaaat tttcaccggc gttgcggtcc acatcagcag ggaaccgaac
      841 atcgcggtaa aggacaccag accgacaaag cgattcatca acgcttccgg tagaccaaaa
      901 tacgccgcca tgccgtggaa tacctgcacc gatccgccgg taaatttcaa ctctttgctg
      961 ctgacgaaga cgttaatcag caccgaggag acggaataca gtacgccgat aaaaatcccg
     1021 gcgaggatga tcactttaac gaacgatttc gaaccgcctt tgacgtcgtt aacgtacacc
     1081 gcgacggact ccgcgccacc tgcggccata aagatccagg tggtaacgcc gaggaacgcc
     1141 cagttgaagt tcgggatcat cgcgtcaacg gtgatggggt cagcaggctg tacgccgcca
     1201 accagcgccg tacctgccag taaaatgtag gagagcgtta acagcagcat cagcgttgaa
     1261 gtgacggagg taattggccc caacattttc gccccgttag tggaaaccca ggtggagaag
     1321 gcgaacagca ccatactgat aatggtggtg gcaaccggcg tcataatata ttcgtagccg
     1381 aggaaggcat acgaagcata agcaataacg cgcggcaaca gcgaggtgaa aaagaacagg
     1441 tttacgaacc agtaggtata ggcagtaata aatgcccaac gcccgcccag cgaacttttt
     1501 acccacgcgt agacaccggc ttctgagttt ttatttaacg aaacaaattc tgcgatgatc
     1561 agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa acatcggtgc cgaggccagt
     1621 ccaagctcaa tattattatt gataacgttg ttaaagctgt aaacggcggc aaaagtcagc
     1681 gagagcaagc cgaatttgcc gattgtatta cgtttggtat cagacataac acatctccga
     1741 aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg ccgtagttgt tatttgttat
     1801 ggaaataacc atcttcgatg gtgactttga gaaccacttt tttgaccgcg ttattgcaga
     1861 taaaccgata tgcttcatgg atatcgcaaa taacgatttg cccttcgtgg acctctacgg
     1921 tttctccgca gccttttaaa tattcgcggt cagtttcgtc acgataatat tccactacct
     1981 gtaatgtctc tttcggcgca tattcaattt tttgctgccc ttgcaggtag taatgcactt
     2041 caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc ggagtctgtc tccacgcggt
     2101 aagtcaatga gtcaccgatg gagtgggcga cgccaggctg aatgttgtcg atattttcaa
     2161 tcgcttcaac gcagcgttgc cacttcttac cagaggcgta aatctggcgg aactgttcta
     2221 agttatcgat gatcctcatt gcttattctc gctgtgcaaa tttgtggaaa agaaccctgc
     2281 gccgaactca tacgacgcca ggctttgcgc ggtagcttct ccgccagaaa ccggcagcaa
     2341 cgtaaagccg tagctgaagt cacggaacca gacgcgccag gagtccagca cctcgctgcc
     2401 ccaggagttg gagccgaggc caagcagctg gtggtcgaga ttcagggtga tgtcatcact
     2461 gcgctgcagc tcgttacagt gctgggcagc gtggatgttt tcctggggat agtgccaggc
     2521 gctgaagtta attgggcgct gcggaaccac cagcagaccg ttgccgtggc ggttagtcag
     2581 tgccgtccag cggacatgct gacggttgcc gttgttctgc gggaagggat agttctcgaa
     2641 catggcatcg acggtgctgc gccagatatc gatgatgtta gcctgctggc tgtcggcgta
     2701 gttttcgccc ggtccacgac cgtaatacgc cacctgatcg tattcgccgt taatccccat
     2761 ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg taacgctcgc cggaaagcgc
     2821 cacgttaacc tggccatcgg cagcgatgcg ccagatgtag gtgcagcgca tcccgaagtc
     2881 aaacaccggc ggtgctataa ccgtgcggct gatgatcaac acttcaccat cgctctgttc
     2941 tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa tgattcggtt gccacagccc
     3001 ttcgtactcc tgcttgtggt tgtcgatcat cggcttgaag aagttgatct ttggctcgcg
     3061 agtcagcagc gactcgccat ttacctgcca ggatgtcggt ttgccactca tttttgagaa
     3121 ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa cgatcgtctt ccagcgtcag
     3181 cggacgcgca ttatttggtg cgaaaggcac tggctgcgcg gtgttttcct tcagcgggaa
     3241 ctgataagtg gcgatagaat gcccggcttc gctgtagcgg gtgcgggaat ctttggtcac
     3301 cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc agcgtgatct gcaagggggc
     3361 ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc gttgcgagcg tttcaccttc
     3421 ggcgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg gtaaaccaca gtttattttc
     3481 gactttcagc tcgccgcgag tcagatccag cgcgtggatt tttaccggcg cgataacctg
     3541 tttgtactct ttcaggcctg gtcccggcgt ctgatcggaa tagatcaaac catcaagaca
     3601 gaagttatag ttgttgggat agtcgccgta gtcgccgccg aatttatacc agacattgcc
     3661 gtggtcgtcc tgtgcctgga tcccgtggtc gcaccactcc cagacataat gaccctgaat
     3721 gcaatcgtgc ttatagaaga cgttctggta ctccgtcagc ccgcccggtc cgttccccat
     3781 cgcatgagca tattcacaga tgatgcgcgg cttcggatgc gggtattcac caaactcatt
     3841 catcagcggc acgcgggtgt acatggtgga aataatatcg accacttcag catcgcgatc
     3901 ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgcttta gccgcatggt acatcgcgcg
     3961 gatgttacag ccatagccgg attcattgcc cagcgaccag atgatgatcg acggatggtt
     4021 tttctgcgcg tgaatatggc gaacaatgcg ctcgacgtag actttttccc actgcggatc
     4081 gtcggtaata cggctgatat cgccgacatt agcaaagccg tgcgattcga cgtcggtttc
     4141 cgccatcaca aacaagccgt agatatcaca cagttcgtaa aaacgcggat cgttcgggta
     4201 gtgagcggta cgcacggagt tgatattgtg ctgcttcatc aactggagat ctttctcgac
     4261 gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg tcgtgacggt tgacgccgtg
     4321 cagcatcaca taacggttat tgatccagaa cagaccgtcg cgcactttga tatcacggaa
     4381 gccaacgcgt tgtggcacca cttccagaac gttgccgttg gcgtctttca gcgtcatgac
     4441 cagatggtaa agataagggg attctgctga ccattgctgc ggctgttcga cagtaaaagc
     4501 aaagctggcg ctggtcagtt tttcaattgc caaatgatca atggcgctgc tgtgcaccac
     4561 gcgttcgcca tcaaacaggg tatattccag cgtcgtgacg acaggggagg cggcgagatt
     4621 ttccagcacc acttcgcagg aaagcgtggc atcgcaatag gcttcgtcaa agtcggtacg
     4681 cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc agataaacat cgcggaagat
     4741 ccccgctgac caccacatat cctggtcttc cacgtaggta gagtccgccc actgcatcac
     4801 gcgcacacac aacaggttgt cgccggtttt aaccatcgcg ctgatgtcaa actctgcggt
     4861 caggcgactg cccttgctga aacccacata ctgaccgtta acatagactt caaaataggt
     4921 ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc cagccgtcgc tgagggtgaa
     4981 aatacgttga taggcaccgg ttgggttatc gctggggaca aacggcacat cgatggggaa
     5041 cggaaaacct tcgtcggtat attgcagttt gccgtgacct tccatttgcc acatggcggg
     5101 gacggtaata tgcccccagt cagccattaa ctcagaggtg aaggcttctg gtacttgcag
     5161 cggatggtca aaaaagtgga aattccactg accgcttaag ggcagaaaca ggctgctggt
     5221 ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat gaaaaaaagt acgcacgcgg
     5281 cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag cgattcatag ggcctcccgg
     5341 tagagagaaa tcggaaccat tcatggtgat ggtagtttgt gtaattgctg gggagtttag
     5401 taaaattttt actaaaaagt agcaggtaaa tgcatttact ttaactggat cacgaaaata
     5461 aacggcagca ctgtccggca aaaacgattg ccggacagtg tgtaagggga tttaacgggt
     5521 cgtgccgcgc agttttaatt tgctgggaac gaagactaac agcggcagcg cgcgaccatc
     5581 gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg aatggatgcg
     5641 cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa cgctgataag
     5701 cgaaatatcc tgtgggatgt tcaggcctcg ttcatgaatt gcccgcagta cgccgatagc
     5761 aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca gcatttgttt
     5821 tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct cttcgcgtac
     5881 cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg ccttgccagg
     5941 ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat agaagtcgat
     6001 gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc tgccgggttc
     6061 gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc gcagggcggg
     6121 cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta agccgctgtg
     6181 ttcataacag ttggtgagct cgatgcccag cttttcgcac tgggtttcaa tgccgtggcg
     6241 gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt agatagccag
     6301 aatatggtgt tggttgactg cacctgtctg gagtttacgg gcactactgg tcttgtactc
     6361 cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg
     6421 atcgtcattt a
//
LOCUS       DACAPB010000221         6399 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.222, whole genome shotgun sequence.
ACCESSION   DACAPB010000221 DACAPB010000000
VERSION     DACAPB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.222"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(33..1979)
                     /gene="nagE"
                     /locus_tag="IT904_004177"
     CDS             complement(33..1979)
                     /gene="nagE"
                     /locus_tag="IT904_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAP3193871.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            2312..3112
                     /gene="nagB"
                     /locus_tag="IT904_004178"
     CDS             2312..3112
                     /gene="nagB"
                     /locus_tag="IT904_004178"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP3193872.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NIKLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            3172..4320
                     /gene="nagA"
                     /locus_tag="IT904_004179"
     CDS             3172..4320
                     /gene="nagA"
                     /locus_tag="IT904_004179"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP3193873.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            4329..5549
                     /gene="nagC"
                     /locus_tag="IT904_004180"
                     /gene_synonym="nagR"
     CDS             4329..5549
                     /gene="nagC"
                     /locus_tag="IT904_004180"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAP3193874.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            5597..6349
                     /gene="nagD"
                     /locus_tag="IT904_004181"
     CDS             5597..6349
                     /gene="nagD"
                     /locus_tag="IT904_004181"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAP3193875.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         1587 a         1688 c         1627 g         1497 t
ORIGIN      
        1 cgcgtcaagc gtcgcatcag gcataaagca gattactttt tgatttcata cagcggtgtt
       61 tgacccgcca caacatggcc ctgagcttta atgatcaagc cactgaaatc gtcgatattg
      121 ctgcaaacca ccgggctaat catcgagcgg gcgttagcgt tcaggtaatc cagatccatt
      181 tccagaatcg gttgccctgc gcttacctgc gcaccctctt ccaccagacg tttaaagcct
      241 ttaccttcca gcgctacggt gtcgataccc atatggacga cgatctccgc gcctttttcg
      301 gtttccaggc agaacgcgtg gttggtgttg aagattttca cgattgtccc tgcggctggt
      361 gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc tttgctggcg
      421 aatgcttcgt caggaacctg atccagtgcc acgacatcac cggtaatcgg cgataccagc
      481 tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc
      541 ggagttgctt cagctgacgc agctgctacc ggaccgcggg caacgacttt cttcatcgca
      601 tcgccgatgg attctgcttt cgcgccaaca atcacctgaa tagtctgttt gttcagtttt
      661 actacaccag aagcacccag acgtttacac atcgtatcgt taacgcgggc agagtcagcc
      721 actgtaaggc gcagacgggt gatacaggcg tcaattgctt tcaggttgtc agtgccgcca
      781 accgcagcaa tatagttggt cgccagttga ttgaggcctt cttcagtgtt gctgttggct
      841 tcttcagtaa cgatctcgtc ttctttatct tcacgacccg gcgttttcag gttgaacatg
      901 cggataacca aactgaacac cacgaagtag atagcgaaga agacaacacc catcaccagc
      961 agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta gtcgatagcc
     1021 cccgcagaga aagagaagcc agcatggata cccagcagtg ttgccacaaa caggctgata
     1081 ccggtcagca gtgcgtgcag gaggtacagc agcggtgcaa ggaacatgaa caggaattcc
     1141 agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag catcccgcca
     1201 accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc acccggcaga
     1261 ccgaacatca taatcgggaa gaagccggac atgaacatcc ccgcggtgcc gtcaccggca
     1321 tagaagcggt tgatatcacc gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc
     1381 tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag cagacggttg
     1441 atgaaaccaa agataccgga acccagcgcg cccgcagaaa cgatccactc gcctcctgca
     1501 tggatagcgt gctgtaccgg cggccagacg taaccaaaaa tggccgccag caccaggcag
     1561 aagaagccgg tggcaatcgg cacaaagcgt ttgccgccga agaagctcag gaagtccggc
     1621 agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt aatgataccc
     1681 gccagtacac ccatgtttat ttctgggttg atggtcacca tcgctttggt taacacaaag
     1741 taacctaccg cacccgccag cgccgccgca cctgcgctgt ctttcgacca gctggatgcc
     1801 acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc ctgggcaata
     1861 aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagtgccgc caccggcagc
     1921 accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa aatattcatc
     1981 ttattccccc tacgagaacc ctatttggct cgtttaaagc cgtattttta ttttgctgca
     2041 aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag agtgtgtgaa
     2101 aatttaattc gtatcgcaaa ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg
     2161 ttatcatcac tcccttttac tggctaaacc agaaaactta ttttatcatt caaaaaatca
     2221 ggtcggattg acgcctgtct gcgcaaatcc aggttacgct taaagatgcc taatccgcca
     2281 acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctga ctaccgctga
     2341 acaggtcggt aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccgac
     2401 tgccgatcgt ccgtttgttc tgggcctgcc aactggcggc acgccaatga ccacctataa
     2461 agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa
     2521 catggacgaa tatgtcggtc tgccgaaaga gcatccggaa agctactaca gctttatgca
     2581 ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac atcaaacttc tcaacggcaa
     2641 cgccccggat atcgacgccg agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa
     2701 aattcatctg tttatgggcg gtgtaggtaa cgacggtcat attgcattta acgaaccggc
     2761 gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa
     2821 ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt
     2881 tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagcc agaaagcact
     2941 ggcactgcag gccgccgttg aaggttgcgt gaaccacatg tggaccatca gctgtctgca
     3001 actgcatcca aaagcgatca tggtatgcga tgaaccttcc accatggagc taaaagttaa
     3061 gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt aattgttatc
     3121 cctgccctgg ctccttgctc agggcaatat tttttataat cgggggtcag aatgtatgca
     3181 ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc ggttgttatc
     3241 gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa
     3301 cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt aaacggctgc
     3361 ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg
     3421 cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat caccaccagc
     3481 gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat
     3541 caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat
     3601 aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg taagaacgcc
     3661 gacgtcatta ctaaagtgac cctggcaccg gaaatggttc ctgcagaagt catcagcaaa
     3721 ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca acgcgacgtt gaaagaagcg
     3781 aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc gatgccgtat
     3841 attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga catttattgc
     3901 ggtattatcg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc taaacgcctg
     3961 aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc cagcaggtgc caacattgaa
     4021 cagttcattt ttgcgggtaa aacaatatac taccgtaacg gactttgtgt ggatgagaac
     4081 ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat
     4141 tgcggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt
     4201 ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag tagccaacct gaccgcattc
     4261 acacctgatt ttaaaatcac caagaccatc gttaacggta acgaggtcgt aactcaataa
     4321 gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc gtaaaacagc
     4381 ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg cggattcaga
     4441 ttgccgagca aagccagctt gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg
     4501 aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc cgcgctatct
     4561 ctatcgtcac cgaaacccgc aatttccacg caatcggtgt acggcttggt cgtcacgacg
     4621 ccaccattac tctgtttgat ctcagcagca aagtgctggc agaagaacat tacccgctgc
     4681 cggaacgtac ccagcagacg ctggaacatg ccctgctgaa tgccattgct cagtttattg
     4741 atagctacca acgcaagctg cgcgagctga tcgccatttc cgtgatcctg cctggacttg
     4801 ttgacccgga cagcggcaaa attcattaca tgccgcatat tcaggtggaa aactgggggc
     4861 tggtagaagc actggaagag cgttttaaag tgacctgttt cgttggtcac gatatccgta
     4921 gtctggcgct ggcagagcac tacttcggtg caagtcagga ttgcgaagac tccattttag
     4981 tgcgtgtcca tcgcggaacc ggggccggaa tcatctctaa cgggcgcatt tttattggcc
     5041 gcaacggcaa cgtcggtgaa attggccata ttcaggtcga accgctgggt gaacgctgcc
     5101 actgcggcaa ctttggctgc ctggaaacta tcgccgccaa cgctgccatt gaacaacggg
     5161 tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc gctggacgac tgtaccatca
     5221 aaactatctg caaagctgcg aacaaaggcg atagcctggc gtcggaagtg attgagtatg
     5281 ttggtcgtca tctgggcaaa accatcgcca ttgccatcaa cctgtttaac ccgcaaaaaa
     5341 ttgttattgc cggagaaatc accgaagccg ataaagtgct gctccctgct attgaaagct
     5401 gcattaatac ccaggcgctg aaggcgtttc gtactaatct gccggtggta cgttctgagc
     5461 tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta
     5521 ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt attgttgtca
     5581 atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat
     5641 gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta tggataaagg
     5701 cctgccgctg gtgttgctga ccaactatcc ttcgcagact gggcaagatc tggcgaaccg
     5761 ctttgccacc gcaggtgtcg atgtacctga cagcgtgttt tatacctctg cgatggcgac
     5821 tgccgatttc ctgcgtcgcc aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact
     5881 gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg atttcgtgat
     5941 tgttggcgaa acgcgttcct acaactggga catgatgcat aaagcagcct atttcgtcgc
     6001 taacggtgca cgttttatcg ccaccaatcc ggacacccac gggcgcggtt tttatcccgc
     6061 gtgtggcgcg ttgtgtgcag gcatcgaaaa aatctccggg cgtaaaccgt tctatgttgg
     6121 taaaccgagt ccgtggatca tccgcgcggc attaaacaaa atgcaggcgc attcggaaga
     6181 aacggtgatt gtcggcgata acctgcgtac cgatattctg gcgggcttcc aggcaggtct
     6241 ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac gatatcgaca gtatgccgtt
     6301 ccgcccaagc tggatttacc cgtcggtcgc tgaaatcgac gttatctgaa tagcaaccac
     6361 gtctgcggac gtggttggct ctgatgacaa acgccaaac
//
LOCUS       DACAPB010000222         6337 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.223, whole genome shotgun sequence.
ACCESSION   DACAPB010000222 DACAPB010000000
VERSION     DACAPB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.223"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..161
                     /locus_tag="IT904_004182"
                     /pseudo
     CDS             1..161
                     /locus_tag="IT904_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(218..988)
                     /gene="yafV"
                     /locus_tag="IT904_004183"
     CDS             complement(218..988)
                     /gene="yafV"
                     /locus_tag="IT904_004183"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAP3193876.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKSGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1142..1615
                     /gene="ivy"
                     /locus_tag="IT904_004184"
     CDS             1142..1615
                     /gene="ivy"
                     /locus_tag="IT904_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAP3193877.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1658..4102)
                     /locus_tag="IT904_004185"
     CDS             complement(1658..4102)
                     /locus_tag="IT904_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP3193878.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4342..4920
                     /gene="lpcA"
                     /locus_tag="IT904_004186"
     CDS             4342..4920
                     /gene="lpcA"
                     /locus_tag="IT904_004186"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAP3193879.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5126..5893
                     /locus_tag="IT904_004187"
     CDS             5126..5893
                     /locus_tag="IT904_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAP3193880.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5864..>6337)
                     /locus_tag="IT904_004188"
     CDS             complement(5864..>6337)
                     /locus_tag="IT904_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAP3193881.1"
                     /translation="KSPEGFYSVQRNQLKPDSRYYKAINIGFPNAYDRAHGYEGKYLM
                     IHGDCVSIGCYAMTNQGIDEIFQFVTGALVFGQPSVQVSIYPFRMTDANMKRHKYSNF
                     KDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         1511 a         1738 c         1635 g         1453 t
ORIGIN      
        1 ttcacgatac cattgccaga gttgtatcct gtatcagtcc tgcaaaattt cacgagtgct
       61 ttattaactg gatgcgtgac tgccattcat caaatgacaa agacgtcatc gcgattgatg
      121 gaaaagcact tccacactcc tatgataaaa gtggtcgcag gagagcgggc tttcgtagtc
      181 ttccccgact ctccccagcc ctaaacacaa cccccactca ccgcaaccta aactcatccg
      241 catcctgcca tgccggaaac ttttctctat attcccgcaa tgccatcatc gacagctccg
      301 catcaatgcg cgttgcctga tgcgcgtcgg cagtagcgat aatctcgcct tgtggattaa
      361 tcacccggct gtcaccgcga taatggcagc cgttgccatc gctgccgacg cgattgcatc
      421 ccgccacata cgcctgattc tcaatcgcgc gggccgtcag caatgcctgc cagtgcagag
      481 agcgcggagc aggccagttg gcgacgtaca gggcgaggtc ataatcgttg agattgcgcg
      541 accacacagg aaaacgtaag tcgtagcaca ccagcggcaa aatacgccag ccgcgccatt
      601 ccacaatcac tcgcgcattg cccgatttat aatgtagatg ctcatctgcc atgcggaaca
      661 gatgacgctt atcataaaaa tgtaccgtgc cgcccggctc aaccagcaaa aagcggttaa
      721 ccgaaccaga ctccgtttgt aatgcaacac tgcctgcaat cagcgcattg cactgctgcg
      781 ccttagctgt catccagttc actacgtcat cttgtgctag cgacgaagct gccgcttcca
      841 tggcaaagcc gctggtaaac atctccggta gaacgatcac atcgcgcccg gtaatacctt
      901 ccagttgacg atcaaaatga cgcaggttgg caggaccatc catccacacc agtggttgct
      961 gcaaaagcgt aatcttcaaa ccaggcacgg tgtacaactc ctttatgcga agggttttat
     1021 aactttaaca ccttatcagg cagttgcctt agcgcagaat aaattgataa caaatgctga
     1081 tattggaaat atctgatttg caaattatcg tgttatcgcc aggctttagg aggttaataa
     1141 catgggcagg ataagctcgg gaggaatgat gtttaaggca ataacgacag tcgccgctct
     1201 ggtcatcgcc accagtgcaa tggcgcagga tgatttaacc attagcagcc ttgcaaaggg
     1261 cgaaaccacc aaagctgcat ttaatcagat ggtacaaggg cataagctgc ctgcctgggt
     1321 gatgaaaggc ggtacttata ctcccgcaca aaccgtaacg ttgggagatg agacgtatca
     1381 ggtgatgagc gcgtgcaaac cgcatgactg tggctcgcaa cgtatcgctg tgatgtggtc
     1441 cgagaaatct aatcagatga cggggctgtt ctcgactatt gatgagaaaa cgtcgcaaga
     1501 gaaactcacc tggctgaatg tgaacgatgc gctttcgatt gatggtaaaa cggtgttgtt
     1561 cgcggcgttg accggcagcc tggaaaacca tccggatggc tttaatttta aataattagc
     1621 ggataaagaa acggagcctt tcggctccgt tattcattta cgcggcttca actttccgca
     1681 ctttctccgg caactttacc ggcttcgtcg ccagctcttc cggatcaaag tcatcaacgt
     1741 taatactgcg cagacggctt tcttcagctt tcaccagaat agcggcttca tctttatcaa
     1801 tcagcccctt caccagcgcg ttgtgcgcca gttcatccag acgggtaaac ggcaggtttt
     1861 tacccagctc tttacagatc cgctgatgaa ttgggtcggc ggcaatcaca tccaccagcg
     1921 cctcttccag caagccaacc ggattatgct cgctcggcgt caggtactga ccgcgaccaa
     1981 tgcgggaacg ggtggcgttc ggcacttgta aaatcttcgc cactttatga tccagcttgt
     2041 cagaaggtgc cagataatga cgtccggtcg ggaagatcac cacattcagc agcccggcaa
     2101 ccacgcggtt cgggaagttt tgcagtaaat catccatcgc ctgttcagcc tgatacagcg
     2161 catcttgtac gccccagtgc accagcggca ggtcggcttc attacggcct tcgtcgtcat
     2221 aacgcttcag cacggcagag gcgaggtaga gctggcttaa aatatccccc agacgggccg
     2281 agatgcgctc gcgacgtttc aggctgccgc ccagcacagc catcgagaca tcagaaagca
     2341 gggcgaggtt ggcgctcagg cggttcaggt gctgatagta gcgtttagtg gcatcgccgg
     2401 ttggcgtgct gctggttaaa ccgcgcgtca ggcccagcca gaagctgcga actttgttgc
     2461 taccgacgtg accgatatgt ttgaacaaca gtttatcgaa cgcgttgacg tcattgttct
     2521 tcgccgcttc catctcttcc agcacgtacg gatggcaacg aatcgctcct tgtccgaaga
     2581 tcatcatgct gcgggtcaga atgttagccc cttcaacggt gatggcaatc ggtgcgccct
     2641 ggtaagcacg cgccaggaag ttgctttgcc cgagcataat gcctttaccg ccggtaatat
     2701 ccatcgcatc aataatcgac tgctgcccgc ggtgggtaca gtgatactta acgatagccg
     2761 acagcacggc aggtttttcg ccgagcataa tgccgtaggt aatcagcgat gccgcagcat
     2821 ccatcacgta ggcattaccg gcaatacgcg ccagcggctc ttcaatccct tccatcttac
     2881 caatagagat tttgaactga cggcgaatgt gagcatacgc gccggttgcc agcgctaccg
     2941 atttcacgcc gccggttgag ttggaaggca gggtgatgcc gcggcctacc gagaggcact
     3001 ccaccagcat ccgccagcct tgcccggcca ttttcggccc gccgatgatg taatcgatcg
     3061 gcacgaagac atctttaccg cgcgtcggtc cgttctggaa cggtacgttc agcgggaagt
     3121 ggcgacgacc aatttccacg cccggcgtgg tggttgggat cagcgcacag gtaatgccta
     3181 aatcttctgc accgccgagt aatttttccg ggtcggagag tttaaacgcc agcccaagca
     3241 cggtcgcaat cggtgccagc gtaatgtagc gtttgttcca ggtcagacgc atccccagca
     3301 cctgctggcc ctgccattcg cccatgcaga caatcccggt gtccggaatc gcgcccgcat
     3361 cggaacccgc ttccgggctg gtcagtgcaa agcaggggat ctcctgacca cgcgccagac
     3421 gcggcagata gtgatctttc tgctcgtcag tgccgtaatg ttgcaacagt tcgcccgggc
     3481 ctaatgagtt tggcacgccg acggtaatcg ccaggatccc gctcacgccg gagagttttt
     3541 gcagcacgcg agactgggca taagccgaga actccagccc gccgtactct tttttgatga
     3601 tcatcgcgaa gaaacgatgc tctttaaggt acgcccacaa ctccggcggc agatccgcca
     3661 gctcatgggt gatctggaaa tcattcgcca tccggcaggc ttcttctacc gggccgtcga
     3721 gaaacgcttg ctcttcggcg gtcaggcgcg gctgcggata gttatgcagc tttttccagt
     3781 ccggcttgcc ctggaacaag tcgccctccc accaggtggt gcccgcatca atcgcttctt
     3841 tctcagtgcg cgacatcggc ggcatcacct tacggaaacc gcgaaatacc ggcgcggaaa
     3901 tcatcgactt acgcataggc gcaaagttaa atggcacgag gataatggcc agaggcacca
     3961 gtacccacgc cgaccacaga ccagcaacgc cgagggcggc tgtccaggcg agcaaaatca
     4021 gactgctgat aaataagctc acgcggtgat agaacaacgc gccgagcagg acaaccgtag
     4081 cgagaatact caaaatcatc ataacgaaaa gccccttact tgtaggaggt ctgaccactt
     4141 gtgatgatat ggttgtagtg gatgtaaaaa catttagcaa tgtgtttaca atataattac
     4201 aacaaagctc acattgttgc tgtttttatc cgcacttcag gtcaaaaagt cctggtcata
     4261 gcacctgccc gtacttctcg cttttggcgg tatccggtac actgcatttt gtctattaca
     4321 tttatgctga aggatatcct catgtaccag gatcttattc gtaacgaact gaacgaagcg
     4381 gcggaaacgc tggctaactt tttaaaagat gacgccaata ttcacgccat tcagcgcgcg
     4441 gcggtcctgt tagcagacag ctttaaagcc ggtggcaaag tgctttcctg cggcaacggc
     4501 ggttcccatt gcgacgctat gcactttgcc gaagagttga ccggtcgcta ccgtgaaaac
     4561 cgtccgggct acccggcgat tgctatttct gacgttagtc atatttcctg cgtcggtaat
     4621 gatttcggtt tcaatgatat tttctcccgc tacgttgaag cggtaggtcg cgaaggcgat
     4681 gtactgctgg ggatctccac ctccggtaac tctgcaaacg tgatcaaagc gatcgcagcg
     4741 gcgcgtgaga aggggatgaa agtgatcacc ctgaccggta aagacggcgg caaaatggct
     4801 ggcacggcgg atatcgaaat tcgcgtaccg cactttggtt atgccgaccg cattcaggag
     4861 attcacatta aagtgatcca tatcctgatc cagttgattg aaaaagagat ggttaagtaa
     4921 gtctggcgta ggccggataa gacgtttacg ccgcatccgg catttgtgcg ctgatgcctg
     4981 atgcgacgct gacgcgtctt atcatgccta caaatctgta cgcgaaccgt aggccgaata
     5041 atgcgttcac gccgcatccg acctgaaaat tcttaaatca atcttcgccg ggggccatgc
     5101 gctcccgctg ttgtggaggt tacccatgtg cgaattgctc gggatgagcg ccaacgtccc
     5161 taccgatatc tgctttagtt tcaccgggct tgtacagcgt ggtggtggaa ccgggccaca
     5221 taaagatggc tggggcatta ccttttacga aggtaaaggc tgtcgcacat ttaaagatcc
     5281 acaacccagc tttaattccc ccatcgccaa acttgtccag gactacccga taaaatcctg
     5341 ttcggtggtg gctcatattc gccaggctaa tcggggcgag gtggcgctgg aaaatactca
     5401 cccgtttacc cgcgagttat gggggcgtaa ctggacttat gcccataacg ggcaactgac
     5461 gggctacaaa tcactggaaa ccggcaactt ccgcccggtc ggtgaaaccg acagcgaaaa
     5521 agccttttgc tggctcctgc ataaattaac gcagcgttac ccgcgcacgc cgggcaacat
     5581 ggcggcagtg tttaaatata tcgcctcact ggcggatgaa ctgcgggcga agggcgtttt
     5641 caacatgctg ctttcggacg gtcgctatgt aatggcgtat tgctcgacta atttacactg
     5701 gatcacccgc cgcgcgccgt ttggcgtggc aacgttgctg gatcaggatg tggaaatcga
     5761 cttcagctcg cagaccacac cgaatgatgt ggtcacggtg attgcgacac agccgctgac
     5821 gggcaatgaa acctggcaaa aaattatgcc aggcgaatgg cgcttatttt gcctcgggga
     5881 gcgtgtagtt tgatgccagt tgtggctgca ccacttcgtg gcttaacggc ttgctgacca
     5941 cgtaacggcc attgaccaca gaaacggtcg gtggcttacg ggtttgctca aagtagtcgt
     6001 agcccggctt cagctgctcc cagaagtcct taaagttgga atatttatgg cgcttcatat
     6061 tggcgtcggt catgcggaat gggtaaatac tcacctgcac gctcggctga ccaaacacca
     6121 gcgcaccagt aacgaactgg aagatctcat caataccctg attggtcatc gcgtagcagc
     6181 cgatggaaac acaatcgccg tggatcatca gatatttccc ttcataacca tgcgcacggt
     6241 cataggcatt ggggaaacca atattaatcg ctttgtagta acggctgtct ggttttaact
     6301 gattacgctg gacgctataa aacccttccg ggctttt
//
LOCUS       DACAPB010000223         6264 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.224, whole genome shotgun sequence.
ACCESSION   DACAPB010000223 DACAPB010000000
VERSION     DACAPB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.224"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..302
                     /locus_tag="IT904_004189"
     CDS             <1..302
                     /locus_tag="IT904_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3193882.1"
                     /translation="MNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEI
                     SVMRYILDGKDNNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            307..3900
                     /gene="evgS"
                     /locus_tag="IT904_004190"
     CDS             307..3900
                     /gene="evgS"
                     /locus_tag="IT904_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAP3193883.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIVLSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYLHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(3956..5101)
                     /gene="yfdE"
                     /locus_tag="IT904_004191"
     CDS             complement(3956..5101)
                     /gene="yfdE"
                     /locus_tag="IT904_004191"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAP3193884.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5175..6119)
                     /gene="yfdV"
                     /locus_tag="IT904_004192"
     CDS             complement(5175..6119)
                     /gene="yfdV"
                     /locus_tag="IT904_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAP3193885.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLFVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
BASE COUNT         1931 a         1391 c         1249 g         1693 t
ORIGIN      
        1 gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa tggctactgc tatttcccct
       61 tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca gcaaaaactc gactccttat
      121 cgaaacaaga aattagtgtc atgcggtata ttcttgatgg caaggataat aatgacattg
      181 ctgaaaaaat gttcatcagc aacaaaactg tcagcactta taaaagtcgc ctgatggaaa
      241 aattagaatg taaatcactg atggatcttt acacattcgc acaacgtaac aaaatcggct
      301 aaccacatga agtttttacc ctatattttt cttctctgtt gtggtctttg gtcgaccata
      361 agtttcgcag acgaagatta catcgaatat cgtggcatca gtagtaacaa ccgtgtcaca
      421 cttgatccac tacgtctgag caacaaggaa ttacgttggt tagcgagcaa aaaaaatctt
      481 gtgattgcag tacataagtc ccaaacggct acgttgttgc ataccgattc gcagcaacgg
      541 gttcgtggta ttaatgctga ttatttaaat cttttaaaaa gagcgttaaa tatcaaatta
      601 acactccggg aatacgcaga tcatcaaaaa gcaatggacg cgcttgcaga aggtgaagtc
      661 gatatagtgt tatcacattt agttacttcg ccgcctctta ataatgacat tgctgcaacc
      721 aaaccattga taattacctt tccggcgctg gtaaccaccc ttcacgactc aatgcgaccg
      781 cttacctcac caaaaccagt aaatattgct cgggtagcaa attacccccc agacgaggta
      841 attcatcaat catttccaaa agcaacaatt atctctttta caaatttata tcaggcatta
      901 gcatccgtct cagctgggca caatgattac tttattggta gtaacatcat taccagcagt
      961 atgatttccc gctatttcac tcactcctta aatgtagtga aatattataa ctcgccgcgt
     1021 caatataatt ttttcttgac cagaaaagaa tctgtcattc ttaatgaagt actcaataga
     1081 tttgttgatg ctttaacaaa tgaagttcgc tatgaagttt cacaaaattg gcttgataca
     1141 ggaaacctgg cctttctgaa caaaccatta gaactcactg aacatgaaaa acagtggatt
     1201 aagcagcatc ccaatttaaa ggtgctggaa aatccttact cgccccccta ttctatgacg
     1261 gatgaaaatg gctcggttcg gggcgttatg ggggacattc ttaatattat taccttgcaa
     1321 acaggtttaa atttttctcc gatcaccgtt tcacacaata tccatgctgg aacacagctt
     1381 agccccggag gatgggatat aatacctggc gctatttata gtgaagatcg agaaaataat
     1441 gttttatttg ctgaagcctt cataacaacg ccttacgttt ttgtcatgca aaaagcgcct
     1501 gacagtgaac aaacattaaa aaaaggaatg aaagttgcca ttccatatta ttatgagctg
     1561 cattcgcaat taaaagagat gtatccggag gttgaatgga tacaggtcga taatgccagc
     1621 gctgcatttc acaaggttaa ggaaggtgaa cttgatgctc tggtcgcgac acagctaaat
     1681 tcgcgttaca tgatcgatca ttactatcct aatgaacttt atcattttct tattcctggc
     1741 gttccgaatg catcgctttc gttcgctttt cctcgcggag aaccggaact taaggatatt
     1801 attaataaag cactgaatgc aattccccca agcgaagttc tgcgcctgac ggaaaaatgg
     1861 attaaaatgc ccaatgtgac cattgacaca tgggacctat atagcgagca attttatatt
     1921 gttacgacat tatccgtttt attagttggc agtagccttt tatggggatt ctacctgtta
     1981 cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag aaaaccaaat atcattccga
     2041 aaagcactct cggattcctt accgaatcca acttatgttg taaactggca aggtaatgtc
     2101 attagtcata atagtgcttt tgaacattat ttcactgcgg attactacaa aaatgcaatg
     2161 ttaccattag aaaacagtga ctcacccttt aaagatgttt tttctaatac gcatgaagtc
     2221 acagcagaaa cgaaagaaaa tcgaacaata tacacacagg tatttgaaat tgataatggc
     2281 atcgagaaaa gatgcattaa tcactggcat acattatgca atcttcctgc aagtgacaat
     2341 gcagtatata tttgtggttg gcaagatatt actgaaacgc gtgatctaat taatgcactc
     2401 gaggtagaaa aaaataaagc gataaaggct accgtagcaa aaagtcagtt tctggcaacg
     2461 atgagtcacg aaataagaac accaataagc tctattatgg gcttcctgga acttctgtcg
     2521 ggttctggtc ttagcaagga gcaacgggtg gaggcgattt cacttgccta cgccaccgga
     2581 caatcactcc tcggcttaat tggtgaaatc cttgatgtcg acaaaattga atcgggtaac
     2641 tatcaacttc aaccacaatg ggtcgatatc cctactttag tccagaacac ttgtcactct
     2701 ttcggtgcga ttgctgcaag caaatcgatc gtattaagtt gcagcagtac gtttcctgaa
     2761 cattacctgg ttaagatcga ccctcaggcg tttaagcagg tcttatcaaa tttactgagt
     2821 aatgctctca aatttaccac cgagggggca gtaaaaatta cgacctccct gggtcacatt
     2881 gatgacaacc acgctgttat caaaatgacg attatggatt ctggaagtgg attatcgcag
     2941 gaagaacaac aacaactgtt taaacgctac agccaaacaa gtgcaggtcg tcagcaaaca
     3001 ggttctggtt taggcttaat gatctgcaaa gaattaatta aaaatatgca gggcgatttg
     3061 tcattagaaa gtcatccagg cataggaaca acatttacga tcacaatccc ggtagaaatt
     3121 agccagcaag tggcgactgt cgaggcaaaa gcagaacaac ccatcacact acctgaaaag
     3181 ttgagcatat taatcgcgga tgatcatccg accaacaggc tattactcaa acgccagcta
     3241 aatctattag gatatgatgt tgatgaagcc actgatggtg tgcaagcgct acacaaagtc
     3301 agtatgcaac attatgatct gcttattact gacgttaata tgccgaatat ggatggtttt
     3361 gagttgactc gcaaactccg tgagcaaaat tcttccttac ccatctgggg gcttacagcc
     3421 aacgcacagg ccaacgaacg tgaaaaaggg ttaagttgcg gcatgaactt atgtttgttc
     3481 aaaccgttga ccctggatgt actgaaaaca catttaagtc agttacacca agttgcgcat
     3541 attgcacctc agtatctcca ccttgatatc gaagccctga aaaataatac ggcgaacgat
     3601 ctacaactga tgcaggagat tctcatgact ttccagcatg aaacgcataa agatctaccc
     3661 gctgcgtttc aagcactaga agctggcgat aacagaactt tccatcagtg tattcatcgc
     3721 atccacggtg cggctaacat cctgaatttg caaaagttga ttaatattag ccatcagtta
     3781 gaaataacac ctgtttcaga tgacagtaag cctgaaattc ttcagttgct gaactctgta
     3841 aaagaacaca ttgcagagct ggaccaggag attgctgttt tctgtcagaa aaatgactaa
     3901 atagcggctc ccacaatgtt caaatgtggg agctatttac cagcacatct tacatttatg
     3961 atgagaactc ctggcgaatt tgttccccat gctggtcgag cgttgccgct cccggcataa
     4021 catgcgggtc cgcgcagccg ctgattttta tcggattacc cggcatcatt attcccccgg
     4081 cttcaatcaa catatttctc gcctgagttt gtggcaattt aatggcctca gccacactta
     4141 ataacggcgc gacgggtaca ccaacttcat gtattctggc taaccaaact tcagctgcct
     4201 gcgtttttaa cgtccgctca atatattgtt taagaatagc ctggttttgt acgcgtaaaa
     4261 tattgctgct aaatcgggga tcattaacca gttccgtaag ctccagtgcc tggcataacg
     4321 cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg cttatcctga gtattgaaaa
     4381 catcaaaagg tgccatgtag ggatggcgat ttcccagacg ttgtggtgac ttccctgtcg
     4441 cgatatatgc catcagacca tgctccagaa aactcagcgt ggcatcaaac atcgctatat
     4501 cgacatgcgc ccctctctgg ctcttttcac ggccataaag tgcactcact attccgctga
     4561 ataaatagac accgccgcat agatccgcaa gagatgtacc aacgcgcact ggcggagcat
     4621 caggatatcc tgtttccatc attatcccgc tcattgcctg aatgatggta tcgtaggcag
     4681 gagcatcttt tagcggaccg gtatgtccga aacctgacga tgaagcatat atgaggcgcg
     4741 ggttgatttc ttgaagcgtt tcccatgaaa accccagttt ttccattgta cctgggcgaa
     4801 aattctcagc taatacatca gcttgtttga gcatatttat aaatatactt ttatcgtgat
     4861 cattctttaa atcaagaacc acactctctt tgccatgatt aataaaactg taatagagtg
     4921 actgtccatc cacataggga ccaaatgtgc gggtatcatc accatgaccc ggcggctcaa
     4981 ctttaattac ccttgcgccc atattacaaa gaagttgagt tccgaaaggt ccattaagga
     5041 catgtgtcat atcgataact aataagcctt caaacgggcc tttgctttca ttatttgtca
     5101 tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac ttcggcattt atgtaaaatc
     5161 aataacatga ttaattatga aaccagtcga ctgacataaa tccacaaggg agccgtgacg
     5221 acaaagccca gtacgctcac agccaatgac gctgtaccag tacgggtgta gacattaaac
     5281 cggctggcaa taatgattcc ggagaatgcc ggcggtaatg cgcctgccag taccatcatc
     5341 tgcagatgtt cgctgttcaa atgacatgcc ataccaacga aaagcagtgc cagtggcatc
     5401 agaatcagct tcaggaaggt gttataagca atttcggcac tgaactcgaa tttatgtgca
     5461 gccagagtca accccgcagc gaataccgct acccctgagt tagccttcgc aatcagatta
     5521 aaggttgggt cccatgctgc cggaattttt accccaacca acaccaggat cgttgccaga
     5581 acaggtgccc atactactgg ctcctttgcc gcagaaatta atgcgctcag attactattc
     5641 ttcttaccat ccgctcctga agaaggattc agcaaataca gaccaatagg aatagtaatt
     5701 gcgttaacaa taatagaaat aattgctacc actaaacctg ttgatacgga atcaccataa
     5761 ataggatcga gaactgcaaa ccccaggaat ccaatggtag gtgaacctgc aattaatgca
     5821 catacagctg cttctgcatg ggtacgttta aaaaatttgt agcaaccgaa ccaggagaaa
     5881 aagaaacatc cgacaataac cacaagtgat acaagggtca gacgagtgtc cgcaaaaatc
     5941 atttccctgt ttgcccgagt aatagataca aatagagccg caggaagcgc gtagtttaat
     6001 accagtttat taaatgcccg agcttgatct tctgaaaatg tttctcgtct gccgctaaaa
     6061 taacccaaca gcatgattac gataataggc aataaatcgc caataaaaaa tgttagcata
     6121 atagtatttc cgataagggt cccctcacca tcgcataatg agttggcaag aggagacgat
     6181 gtcagtattt aattaccagc gacttgtttt gggttaagtt tggtaatatg gccactttca
     6241 gttcctgctg cagggtcgat gacc
//
LOCUS       DACAPB010000224         6205 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.225, whole genome shotgun sequence.
ACCESSION   DACAPB010000224 DACAPB010000000
VERSION     DACAPB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.225"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            65..397
                     /gene="cusF"
                     /locus_tag="IT904_004193"
     CDS             65..397
                     /gene="cusF"
                     /locus_tag="IT904_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAP3193886.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            413..1636
                     /gene="cusB"
                     /locus_tag="IT904_004194"
     CDS             413..1636
                     /gene="cusB"
                     /locus_tag="IT904_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAP3193887.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            1648..4791
                     /gene="cusA"
                     /locus_tag="IT904_004195"
     CDS             1648..4791
                     /gene="cusA"
                     /locus_tag="IT904_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAP3193888.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            4893..>6205
                     /gene="pheP"
                     /locus_tag="IT904_004196"
     CDS             4893..>6205
                     /gene="pheP"
                     /locus_tag="IT904_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAP3193889.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWMEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSA"
BASE COUNT         1352 a         1517 c         1846 g         1490 t
ORIGIN      
        1 cgcttgtcgc cttcctgcaa ctcgaattat ttagagtatg acttttaact ccaggagaga
       61 ataaatgaaa aaagcactgc aagtcgcaat gttcagtctg tttaccgtta ttggctttaa
      121 tgcccaggct aacgaacatc atcatgaaac catgagcgaa gcacaaccac aggttattag
      181 cgccactggc gtggtaaagg gtattgatct ggaaagcaaa aaaatcacca tccatcacga
      241 tccgattgct gctgtgaact ggccggagat gaccatgcgc tttaccatca ccccgcagac
      301 gaaaatgagt gaaattaaaa ccggcgacaa agtggcgttt aattttgtcc agcagggcaa
      361 cctttcttta ttacaggata ttaaagtcag ccagtaaccc aggtttaatg agatgaaaaa
      421 aatcgcgctt attatcggca gcatgatcgc gggcggtatt atttctgcgg caggttttac
      481 ctgggttgca aaggcggaac cgcctgcaga aaaaacgtcg accgcagaac gtaaaatctt
      541 attctggtac gacccaatgt atcccaatac gcggttcgat aaaccaggta aatcgccgtt
      601 tatggatatg gatctggtgc cgaaatatgc cgatgaagag agttctgcgt ctggtgtgcg
      661 cattgacccg actcagacgc agaatctggg ggtgaaaacg gctaccgtca cgcgcggacc
      721 gctgactttt gcccagagtt tcccggcgaa tgtcagttac aacgagtatc agtatgccat
      781 tgtgcaggct cgcgctgccg ggtttatcga caaggtgtat ccgcttaccg tgggcgataa
      841 agtacaaaag ggcacaccgc ttctcgacct gaccattcct gactgggtgg aagcgcagag
      901 tgagtattta ctgctgcgcg aaaccggcgg tacggcgacc cagactgaag gcattcttga
      961 gcggctgcga ctggcgggaa tgccggaggc ggatattcgc cgcctgatcg ccacgcaaaa
     1021 aatccagact cgctttacgc tcaaagcgcc cattgatggc gtgatcaccg cgtttgatct
     1081 gcgcgcggga atgaatatcg ccaaagataa cgtggtagcg aaaattcagg gtatggaccc
     1141 ggtgtgggtc actgctgcga tcccggagtc tatcgcctgg ctggtgaaag atgcctcgca
     1201 gtttaccctc accgttccgg cgcgaccgga taaaacactc accatccgca aatggacgct
     1261 gctacctggc gtggatgccg cgacccgcac gctgcagctg cgtctggaag tcgacaacgc
     1321 cgacgaggcg ctaaaaccgg gcatgaacgc ctggttgcaa ctcaacaccg ccagcgaacc
     1381 gatgctgctc attccgtcac aagcgctgat tgataccggc agcgaacagc gggtgattac
     1441 cgttgatgcc gacgggcgct ttgtaccgaa acgcgttgct gttttccagg catcgcaagg
     1501 cgtcaccgca ttacgctctg gtctggcgga aggtgaaaag gtggtttcca gcggcctgtt
     1561 cctgattgat tccgaagcca atatttctgg cgcactggag cggatgcgct ctgaaagtgc
     1621 tacccatgcg cattgaggga ataaccaatg attgaatgga ttattcgtcg ctcggtggcg
     1681 aaccgttttt tggtgctgat gggcgcgttg tttctgagca tctggggcac ctggaccatc
     1741 attaatacgc cagtggatgc gctgccggat ctctccgatg tgcaggttat tattaaaacc
     1801 agctatcccg gtcaggcacc acaaatcgtt gaaaatcagg tgacttatcc gctaaccacc
     1861 accatgttgt cggtgcctgg cgcgaagact gtgcgcggtt tctcacagtt tggcgactct
     1921 tatgtgtatg tcattttcga agatggcacc gatccgtact gggcgcgttc gcgggtgctg
     1981 gagtacctca accaggtaca gggtaagctg cctgcgggag tcagcgccga gctggggcca
     2041 gatgccacgg gtgttggctg gatctatgaa tatgcactgg tggatcgcag cggtaagcac
     2101 gatctggccg atttacgctc attacaggac tggtttctca aatatgagct gaaaaccatc
     2161 cctgacgttg cggaagtggc gtcggtgggc ggtgtggtga aagagtatca ggtggttatc
     2221 gatccccagc gcctggcgca gtatggcatc agtctcgccg aagtaaaaag cgcgctggat
     2281 gcttcaaacc aggaagcggg cggttcgtcg atcgaactgg cggaagcgga atatatggtg
     2341 cgcgccagcg gctatctgca aacgctcgac gactttaatc acatcgtttt aaaagccagt
     2401 gaaaatggcg tgcccgttta tctgcgcgat gttgcgaagg tccagattgg cccggagatg
     2461 cgccggggca ttgccgaact aaacggcgaa ggcgaagtgg cgggcggggt ggtgatcctg
     2521 cgatccggca aaaacgcccg agaagtgatc gccgccgtga aggacaaact ggaaacgctg
     2581 aaaagtagtc tgccggaagg cgtggagata gttacaacat acgatcgcag ccagctcatt
     2641 gaccgcgcca tcgacaacct cagcggcaag ttgctggaag agtttattgt ggtggcggta
     2701 gtctgtgcgc tgtttctctg gcatgtgcgc tcggcgctgg tggcgattat ttcgttgccg
     2761 ctggggttgt gtattgcttt tattgtcatg cacttccagg gactgaatgc caatattatg
     2821 tcgttgggtg gcattgcgat tgccgtcggg gcgatggtcg atgctgctat cgtcatgatc
     2881 gagaatgcgc ataaacggct ggaagagtgg cagcaccagc atcctgacgc cacgctggat
     2941 aataaaacgc gctggcaggt gatcaccgat gcgtctgttg aagtggggcc ggcgctattt
     3001 atcagtctgc tgattatcac gttgtcgttt atcccgatct tcaccctgga agggcaggaa
     3061 gggcgtctgt ttggcccgtt ggcgttcacc aaaacgtatg cgatggcggg tgcggcgctg
     3121 ctggcgatcg tagtgatccc gatcctgatg ggctactgga tccgtggcaa aattccgccg
     3181 gaaagcagta acccgctcaa tcgctttttg attcgtgttt atcatccgct gttgctgaaa
     3241 gtactgcact ggccgaaaac cacgctgctg gtggcggcgc tttcggtgct gacggttctc
     3301 tggccgctca ataaagttgg cggggaattt ttaccgcaga tcaatgaagg cgacttgttg
     3361 tatatgccat cgacgctgcc ggggatttcc gcagcagagg cggcgagtat gctgcaaaaa
     3421 accgacaagc taattatgag cgtacctgaa gtggcgcggg tatttggcaa aaccgggaaa
     3481 gcggaaaccg ccaccgattc tgctccgctg gagatggtag aaacgaccat ccagcttaag
     3541 ccgcaggagc agtggcggcc aggcatgacg atggacaaaa tcattgagga actggataac
     3601 accgtgcggc tgccggggct ggcgaatctg tgggtgccgc caattcgtaa ccgtatcgat
     3661 atgctctcaa ccggcattaa aagccccatc ggcattaaag tttccggcac tgtgctggcg
     3721 gatatcgacg cgatggctga gcaaattgaa gaagtagcgc gaacggtgcc aggcgtagct
     3781 tctgcgcttg ccgagcggct ggaaggtggg cgctatatca acgttgagat taaccgtgaa
     3841 aaagccgcac gttacggtat gacggtggcg gatgtgcagt tgtttgtgac ttctgcggtg
     3901 ggcggggcga tggttggcga aacggtggaa gggattgccc gttatccaat taatctgcgt
     3961 tatccgcaaa gctggcgcga tagtccgcag gcactgcgcc agctgccgat cctgacgccg
     4021 atgaagcagc aaatcaccct ggcagacgtg gccgacatta aagtctctac cggaccgtcg
     4081 atgctgaaaa ccgagaatgc gcgcccgacg agctggattt atatcgatgc ccgcgatcgt
     4141 gacatggtgt cggtggttca cgatttgcaa aaagcgatag ctgaaaaagt gcagttaaaa
     4201 ccgggcacca gcgtggcatt ctccgggcag ttcgagctac tggagcgcgc caaccataag
     4261 cttaaactca tggtgccgat gacgttgatg attatcttcg tgctgttgta tctggcgttc
     4321 cgtcgggtgg gcgaagcgtt gctgattatc agcagcgtac cgtttgcgct ggtgggcggc
     4381 atctggttgc tgtggtggat gggctttcat ctttccgtgg cgacgggcac tggctttatc
     4441 gccctcgccg gggtcgccgc cgaatttggc gtggtgatgc tgatgtattt acgtcacgcc
     4501 atagaggccg tgccgtcgtt gaataatcca caaacattca gcgagcagaa gctggatgag
     4561 gcgttatatc acggcgcggt cctgcgcgtg cgcccgaaag cgatgacggt ggcggtgatt
     4621 atcgccggtc tgctgccgat tctgtgggga acgggggctg gttcagaggt gatgagccgg
     4681 attgccgcgc cgatgattgg cggcatgatc accgcacctt tgctgtcgct gtttattatc
     4741 ccggcggcgt ataagctgat gtggctgcac cgacatcggg tacggaaata aaagcaggat
     4801 accccgttta accgtgtgga ttgtgtcttg cgacgatggg cactaaatgt taaaaggtgc
     4861 ccctcaacaa aaaagacaca caggggaaag gcgtgaaaaa cgcgtcaacc gtatcggaag
     4921 atactgcgtc gaatcaagag ccgacgcttc atcgcggatt acataaccgt catattcaac
     4981 tgattgcgtt gggtggcgca attggtactg gtctgtttct tggcattggc ccggcgattc
     5041 agatggcggg tccggctgta ttgctgggct acggcgtcgc cgggatcatc gctttcctga
     5101 ttatgcgcca gcttggcgaa atggtggttg aggagccggt atccggttca tttgcccact
     5161 ttgcctataa atactgggga ccgtttgcgg gcttcctctc tggctggaac tactgggtaa
     5221 tgttcgtgct ggtgggaatg gcagagctga ccgctgcggg catctatatg cagtactggt
     5281 tcccggatgt tccaacgtgg atttgggctg ccgccttctt tattatcatc aacgccgtta
     5341 acctggtgaa cgtgcgctta tatggcgaaa ccgagttctg gtttgcgctg attaaagtgc
     5401 tggcgatcat cggtatgatc ggctttggcc tgtggctgct gttttctggt cacggcggcg
     5461 agaaagccag tatcgacaac ctctggcgct acggtggttt cttcgccacc ggctggaatg
     5521 ggctgatttt gtcgctggcg gtaattatgt tctccttcgg cggtctggag ctgattggga
     5581 ttactgccgc tgaagcgcgc gatccggaaa aaagcattcc aaaagcggta aatcaggtgg
     5641 tgtatcgcat cctgctgttt tacatcggtt cactggtggt tttactggcg ctctatccgt
     5701 ggatggaagt gaaatccaac agtagcccgt ttgtgatgat tttccataat ctcgacagca
     5761 acgtggtagc ttctgcgctg aacttcgtca ttctggtagc atcgctgtca gtgtataaca
     5821 gcggggttta ctctaacagc cgcatgctgt ttggcctttc tgtgcagggt aatgcgccga
     5881 agtttttgac tcgcgtcagc cgtcgcggtg tgccgattaa ctcgctgatg ctttccggag
     5941 cgatcacttc gctggtggtg ttaatcaact atctgctgcc gcaaaaagcg tttggtctgc
     6001 tgatggcgct ggtggtagca acgctgctgt tgaactggat tatgatctgt ctggcgcatc
     6061 tgcgttttcg tgcagcgatg cgacgtcagg ggcgtgaaac acagtttaag gcgctgcttt
     6121 atccgttcgg caactatctt tgcatcgcct tcctcggcat gattttgctg ctgatgtgca
     6181 cgatggatga tatgcgcttg tcagc
//
LOCUS       DACAPB010000225         6196 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.226, whole genome shotgun sequence.
ACCESSION   DACAPB010000225 DACAPB010000000
VERSION     DACAPB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.226"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            36..167
                     /locus_tag="IT904_004197"
     ncRNA           36..167
                     /ncRNA_class="other"
                     /locus_tag="IT904_004197"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(351..1028)
                     /gene="narI"
                     /locus_tag="IT904_004198"
     CDS             complement(351..1028)
                     /gene="narI"
                     /locus_tag="IT904_004198"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP3193890.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1028..1738)
                     /gene="narJ"
                     /locus_tag="IT904_004199"
     CDS             complement(1028..1738)
                     /gene="narJ"
                     /locus_tag="IT904_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP3193891.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1735..3273)
                     /gene="narH"
                     /locus_tag="IT904_004200"
     CDS             complement(1735..3273)
                     /gene="narH"
                     /locus_tag="IT904_004200"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP3193892.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIECAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3270..>6196)
                     /locus_tag="IT904_004201"
     CDS             complement(3270..>6196)
                     /locus_tag="IT904_004201"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP3193893.1"
                     /translation="KGTKTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLRE
                     FHLDNPSQYFTDYVRRYTDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKT
                     VAFNTNGEMVAPNGSIGFRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFP
                     YFGGDGTEHFNKVELENVLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDV
                     NCATSYDDVKAYTPAWAEQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWY
                     HLDMNYRGLINMLIFCGCVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMN
                     STSYFYNHSSQWRYETVTAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTN
                     PLTIAGEAEKAGMNPVDYTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGK
                     GHEFMLKYLLGTEHGIQGKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCL
                     YSDIILPTATWYEKDDMNTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCV
                     GHLGKETDIVTLPIQHDSAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATY
                     ERFTSIGPLMEKIGNGGKGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEM
                     ILTLAPETNGQVAVKAWAALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTW
                     SGLEDEHVSYNAGYTNVHELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSV
                     KEVIGQKSNGNPEKALNFLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLG
                     IADNDWIEVFNSNGALTARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNS
                     VTRITPKPTHMIGGYAHLAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQES
                     VK"
BASE COUNT         1363 a         1794 c         1594 g         1445 t
ORIGIN      
        1 agtccctgaa cttcccaacg aatccgcaat taaatattct gcccatgcgg ggaaggatga
       61 gaagcttcga ccaaggttcg actcgagcgc cagcgagaga gcgttgccgc aggcaacgac
      121 ccgaagggcg aagcgcgcag cgctgagtaa tccttccccc accaccatca cttcctgaaa
      181 tactcaaaac ctgttactcc gagctaattt tgcccgatgg caccgcgttt accggaccta
      241 caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac cccgcaccgt
      301 gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattctc ttagtgacga
      361 gcgcgcacca actgatactt acgtgtcaga tactccaccg gtacgctcca gatgtgcacc
      421 agacgcgaga acgggaacag caggaataac gtcatcccca acaccaggtg cagacggaag
      481 ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac caccgactgc
      541 gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga gaacggaatg
      601 gtcaacaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat atccgctccg
      661 gtagtggttg cacgcacgcg tgggctgaac agacgacgct tcagcaacag cacgccgcca
      721 atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg tttcacttca
      781 atcggcagcc aggcttcata catccagtgc ggcgtcagca taccgaagaa gtgaccaaca
      841 aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc tttgcgatcc
      901 agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg cagccagcta
      961 ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa catattcagg
     1021 aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg acggcaccgg
     1081 caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca gcaaagaatt
     1141 taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg cgcgcttcgt
     1201 cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat ttcagcagca
     1261 gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc agcaatgcca
     1321 gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc tgcgacaggt
     1381 actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc aaaccgtgct
     1441 gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat tcaccgtgaa
     1501 catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg ctgtactggg
     1561 cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc agcgcatggg
     1621 catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct tgttgatgct
     1681 gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt tcgatcatgg
     1741 atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt tgaacagatt
     1801 gaatttggta tctgaaccgt ggcagccatc accaaaggta aagccgcagc catttttctc
     1861 cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc gatcttcgta
     1921 gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca gaccgacctc
     1981 ttccagcgca cgggtatcaa ctttaccgtc aacggtttca gcacgtttgt aatgacgcat
     2041 cgccagcata cgtttcagtg cgcgcagtac cggtttggta tcaccggcgg tcagcagatt
     2101 cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaatgc cgttgctacc
     2161 cagctcaccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt accagaccat
     2221 cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca ttgccatttt
     2281 ataaaccggc gactgctgtg cggcttcaat aacgctcagc ggaataccgt ctttaatcgc
     2341 ctgctcgatg actttcggat cgttcggatc gaggaacacc tccagctgac gctggtaaag
     2401 atctttctcg ttctcggtgc tggctgcaca ttcaatcgcg tcggcatcgt acagcagcac
     2461 gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct gacccgcttc
     2521 aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt tgaagtagat
     2581 ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat cctggtcgat
     2641 caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg tcgccacaca
     2701 tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg tgttttcgaa
     2761 ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca gtttgtcaaa
     2821 ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca tccgttcccc
     2881 ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg gcgcggtatg
     2941 caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg gcagatgcgg
     3001 gttagcgaag attttaccca gcagcatggc gcggttaccc atgcgcggct gcagtttgcc
     3061 attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt cagtcgggaa
     3121 gccctgaccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc cttcacggct
     3181 ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact tatcgagatt
     3241 cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac ctggtcattg
     3301 ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa ctcatcgcgg
     3361 ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata gccgccgatc
     3421 atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc acgctgttgg
     3481 gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat catggtcatc
     3541 cctgccggaa cacgctggct gacaaccgca cgggcagtca gagcaccgtt gctgttgaat
     3601 acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc actcaaccag
     3661 accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta ggtggagtgg
     3721 ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt gccgttggat
     3781 ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata aaccagcagg
     3841 ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg accagagagc
     3901 gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga aacgtgttca
     3961 tcttccagac cagaccaggt cgggctggag ataattttgc gcggctgtgc ctgaatatcg
     4021 cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc acgaccggta
     4081 aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc cggtgccagt
     4141 gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc tttcgccgga
     4201 ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg ggtgttccag
     4261 gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga agtaaagcgt
     4321 tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt tttgcctggg
     4381 atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc cagttcagcg
     4441 gcagagtcat gctggatagg cagcgtgacg atgtcggttt ctttacccag atggccgacg
     4501 cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct tttcgcttcc
     4561 caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc cgaagtattc
     4621 atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata gagacaggtg
     4681 ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag accattgtcc
     4741 tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt accctggata
     4801 ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc ggaagaaccg
     4861 agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt ttctggttgt
     4921 tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc caccggattc
     4981 atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa ctgcggtgca
     5041 gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg tccggtatag
     5101 cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc ataacgccac
     5161 tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg acgctgccag
     5221 tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc ctgacctaca
     5281 tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag catgttgatc
     5341 agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc gacgataatc
     5401 atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat gcgaataatc
     5461 tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc tttcacatcg
     5521 tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta gtttgccagc
     5581 gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg caggcgtttc
     5641 accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg ttccgtgccg
     5701 tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc cagcaggctc
     5761 agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt ccatttgccc
     5821 ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc attggtatta
     5881 aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac cagatcagca
     5941 gcgcgcaaca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat caccagcatc
     6001 ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt gtcgaggtgg
     6061 aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt gccctgtttc
     6121 ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt gacggcaaca
     6181 gttttggtgc ctttgt
//
LOCUS       DACAPB010000226         6130 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.227, whole genome shotgun sequence.
ACCESSION   DACAPB010000226 DACAPB010000000
VERSION     DACAPB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.227"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..231
                     /locus_tag="IT904_004202"
     CDS             <1..231
                     /locus_tag="IT904_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP3193894.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="IT904_004203"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="IT904_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP3193895.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="IT904_004204"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="IT904_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP3193896.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="IT904_004205"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="IT904_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP3193897.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="IT904_004206"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="IT904_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP3193898.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="IT904_004207"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="IT904_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP3193899.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="IT904_004208"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="IT904_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP3193900.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..>6130
                     /gene="rpoB"
                     /locus_tag="IT904_004209"
     CDS             4348..>6130
                     /gene="rpoB"
                     /locus_tag="IT904_004209"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP3193901.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKV"
BASE COUNT         1539 a         1480 c         1619 g         1492 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggagtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaat gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctaga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagat gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgtc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caactcttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc actgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 attgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagcg gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga
//
LOCUS       DACAPB010000227         6107 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.228, whole genome shotgun sequence.
ACCESSION   DACAPB010000227 DACAPB010000000
VERSION     DACAPB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.228"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(29..718)
                     /locus_tag="IT904_004210"
     CDS             complement(29..718)
                     /locus_tag="IT904_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP3193902.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(812..2959)
                     /gene="fdhF"
                     /locus_tag="IT904_004211"
     CDS             complement(812..2959)
                     /gene="fdhF"
                     /locus_tag="IT904_004211"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2540..2542),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HAP3193903.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(3157..4623)
                     /gene="mdtP"
                     /locus_tag="IT904_004212"
     CDS             complement(3157..4623)
                     /gene="mdtP"
                     /locus_tag="IT904_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAP3193904.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYSMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLATGVAQLYYSMQASYQMLDLLEQTHDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IRPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLHTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(4620..>6107)
                     /gene="mdtO"
                     /locus_tag="IT904_004213"
     CDS             complement(4620..>6107)
                     /gene="mdtO"
                     /locus_tag="IT904_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAP3193905.1"
                     /translation="ALNDRLDDAISHLTDSLAPLPETRIEREALALQKLNVFCLADDA
                     NWRTQNAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHSIAEG
                     QCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPAAKPPSMAADAFTNP
                     DYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLRFGGAF
                     CGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALA
                     TLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSK
                     VMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEERALLIERSQTV
                     IRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATALSRSPQITLEE
                     TPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
BASE COUNT         1337 a         1678 c         1609 g         1483 t
ORIGIN      
        1 acgcttgccg cgtcttatca ggcctatctt aaccgttggt taatttttca aactcttcac
       61 acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc ttttgcttgt
      121 tcttctcgat aaaaccttct tcaccgttta agaacatcat tcccgcccag tactcggaat
      181 aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg tcatcgtcgg
      241 ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca tcgacggcag
      301 agtcgttctc cagatcttcc gaggcgtttt ctaacagcga aattgccttt ggataatccg
      361 gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct tctttactgc
      421 ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga ctgaccggat
      481 tggtgatttt gatctgcgcc agcagcgcgc aggcatcagc atcaccgctg tcggcggctt
      541 tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg tcggcaagaa
      601 aatattgtgc gcgtcggtcc ccggcctctg ctgcctttaa atactggctg ccaggctcgg
      661 agtcgttggc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt tttttcattt
      721 tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat aaaaaaacag
      781 cctccgaaag gaggctgtag aaaggacggt attacgccag tgccgcttcg cgcaggcgag
      841 ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga tcggcgatcg
      901 gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt cgtaatcggg cttaagtttt
      961 cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa atcgcccctt
     1021 tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc gagtgcaccc
     1081 aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg atttgtgcgt
     1141 agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc gaacggcaag
     1201 agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg gtgagtttgt
     1261 cgattggcgc tacccagtcg caggtgaaga actgcgccag accgttcggg gtatcaaact
     1321 tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg caaggccact
     1381 gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc gggcacagat
     1441 gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa cccatacggg
     1501 tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt tcaaccgcct
     1561 tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc tcgccccacg
     1621 acgttgacgg taaaataaca tccgccgccg acgcggtttt ggtcataaag atgtcctgaa
     1681 cgataaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc gcgtcagttt
     1741 gcagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct gcgcggtgcg
     1801 gtagctcgct gatgcgatag ccggtatgcg ctggcaggct ttccacgccc caggctttgg
     1861 cgaatttctc gcggttagcc ggatctttca cgtactggta tcccggatac gtatccggca
     1921 gcgcgcccat atcgcaggca ccctgtacgt tgttctgacc acgaaccggg ttaacacccg
     1981 catgtggctt accgaggtta ccggtcagca tcgcgaggct ggtcagagaa cgcacggttt
     2041 ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg cttttcgcct
     2101 gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacgccggtg atatcttcaa
     2161 ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct tctgtacggg
     2221 aagcgacgaa tgctttgtcg tacaaatttt cttcaataat gacatggccc atcgcattca
     2281 acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg cgcgcggttt
     2341 caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg ttaattacgt
     2401 gattcgctac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac actaaatcgg
     2461 tattatcaat ttcgttaata gcattgctca ttgcgccatt accgaccgat tggtgcagac
     2521 ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg gtaccaataa
     2581 cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca cgcgaggagc
     2641 cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg cgctcggcaa
     2701 cgtaattcag tgcctcatcc caggaaacag gttcgagttt gccgccacgc tggcgacgga
     2761 tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaag tcccagccat
     2821 aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc tccgcccgga
     2881 cgattttgcc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa taggggcaaa
     2941 ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat acgcgctctc
     3001 gactacagta tgcatctttt atgccacatt ttatgtgggg tcattccctg atattacggg
     3061 cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac gtgacgaaac
     3121 gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga cgacgggacc
     3181 tgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca cccggcggct
     3241 gtccagcatc agtaatgaca tctcttcggc aagcactggc aaccgggctt cggtggcctg
     3301 taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg taaagcgcgt
     3361 ggcttccacg cgttcagcct gcatttctcg ctcgtcgttg agcgtttgca gacgcgtgcc
     3421 gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt caatcatcat
     3481 gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt caaacagcgg
     3541 caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata aggtatgcag
     3601 atggatggag tccagaccga aaaacgcttt gatatcaaag ctcggataga acaacgcccg
     3661 cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt gcagatccgg
     3721 gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga cttgcggtaa
     3781 tgccaccggt ctgatctccg gcatatcgct ggctcccgcg ccaatcaatg cacgcagaga
     3841 ttctcgcgtt tcggtgattt gccctttgac ggcaacaatt tgtttatcga ccgccagaat
     3901 ctgtgcccgc gcgccgtgga aaggcacttg cgcttccaga ccgtgcgcca ctttactctg
     3961 gtgcgctttc accgcgtaat caatcacatc gtgagtttgt tctaacagat cgagcatctg
     4021 atagctggcc tgcatactgt aataaagctg cgctacgccc gtggccagcg atagctctac
     4081 tgctgcggtt tctgccagcg cggcattatg cgcgccaatg gcggcggcaa ccgctgagcg
     4141 atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa cctactgtgg cttccgtata
     4201 gtacggcccg tccataccca gtgctggcgc atccattgaa taagggctta aaaagccgtt
     4261 cgccgagaca cgttggcggt tgagcatccc taacgctgcg acctgtaatt gtgaaccggc
     4321 atctaacaaa tcggcctggg actgcgcttt ttcttcccgc agtttcgctt cggcgagggt
     4381 gtgtgaacca cttagcgtcc gttggatcag cgcatccagc tgcgggtcat tgagttgttt
     4441 ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcgtcgg ccagtttgat
     4501 ttgttccggt ttgagctgtt gatgaggtgc agaatcctta cgtaccaggg cacagccgga
     4561 aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt gacgattgat
     4621 cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc tgtccagtct
     4681 ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt gggcaggatg
     4741 gcctgagagg cgggtgtctc ttcaagcgtt atttgaggag aacggctgag tgcggttgcc
     4801 agaccggcag cgtatttctc cagggcgtcg gcaaactgac ctgctctgtc cggctgtagt
     4861 gcgttatcca gcgcctgcgc cgagttccag gtggcatccc aggcgtgaag aagatcgcgg
     4921 ccctgacgaa taaccgtttg cgaacgttca atcagtaatg cgcgttcttc gctgtccagt
     4981 tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc cgcatgcaga
     5041 cctatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg tgggatccgc
     5101 attactttac ttaacatacc cagcgcgcca gccagttttt gcggcagcgt gcgcgcttca
     5161 ctctcaggcc agacaaaggt gtaaatcacc gcggacacca cggtaccaat gatgataccc
     5221 agggcgcgat cgcgaatttc caccaggtcg tacaccgggc caaaaacgtt ttcgagcgtg
     5281 gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagaacgttc agagctggtg
     5341 gcaatccatg cgcccaacag gaaaataggt gccagcacaa acagcaattc gacaatattg
     5401 tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc gcaaaaagcc
     5461 ccgccaaaac gcagcaccat cttctggtac gacgaaccga cattcgggtt agcgacgatc
     5521 acgcaggtca gcatacaggt gtgaatgcct tcccagtcga cgccgctgta gaaggtgtaa
     5581 cagatcaaac aggcgagcag cgtttttacc gcgtagcgca tatagtctgg attggtaaaa
     5641 gcatcggcgg ccattgatgg tggtttggcg gcgggcgttg gcggcgtatt cgggtccatc
     5701 tgacccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg tgccgccatc
     5761 gcttcacttt cactgatccg ccagtcgctt tgccagcact gaccttcggc aatggaatgc
     5821 tgcagcttgt tgatttctga ggctaatttt tgtcggaatt caataattgc ctgagaatca
     5881 gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac cgttgccacg
     5941 cagctttgcc accatgcgtt ttgagttcgc cagttggcat cgtccgcgag gcaaaagaca
     6001 ttgagttttt gtagcgccag cgcctctctt tcaatccgcg tttcgggtag cggtgcgagg
     6061 ctgtctgtca ggtgactaat ggcatcatca agccgatcat taagcgc
//
LOCUS       DACAPB010000228         6089 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.229, whole genome shotgun sequence.
ACCESSION   DACAPB010000228 DACAPB010000000
VERSION     DACAPB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.229"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(47..736)
                     /gene="pepE"
                     /locus_tag="IT904_004214"
     CDS             complement(47..736)
                     /gene="pepE"
                     /locus_tag="IT904_004214"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAP3193906.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            948..1820
                     /gene="rluF"
                     /locus_tag="IT904_004215"
     CDS             948..1820
                     /gene="rluF"
                     /locus_tag="IT904_004215"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAP3193907.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(1953..2225)
                     /locus_tag="IT904_004216"
     CDS             complement(1953..2225)
                     /locus_tag="IT904_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAP3193908.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     DYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2478..3827)
                     /gene="lysC"
                     /locus_tag="IT904_004217"
     CDS             complement(2478..3827)
                     /gene="lysC"
                     /locus_tag="IT904_004217"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAP3193909.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDSRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4352..6001
                     /gene="pgi"
                     /locus_tag="IT904_004218"
     CDS             4352..6001
                     /gene="pgi"
                     /locus_tag="IT904_004218"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP3193910.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1547 a         1574 c         1559 g         1409 t
ORIGIN      
        1 tcaggcacta tgaacaagat gccggatcat gcatccggca attcacttaa aaacggtgac
       61 cagcttccag cggaaccgct tcttcaccag ccttaaacac ataagtggtg ttcgggccac
      121 ccagcgtagc gtgaccttta ctcactgtga tccagttacc ttccggtaga ccaataatcg
      181 tcagttctgg cgcgacgacc agcagttcgc gaatacgctg ctcacgggtt tcacctttat
      241 ggccttccgg cagcgcgttg gtgaagtgcg ggttgatttg cagcgggaac agatttagcg
      301 catcgaaacc ttgcggatcg acaatcggca tatcgttggt ggtacgaata gttgggcaag
      361 caaggttagc gcctgcgctc cagccaatat acagcgcgcc acgtttcacc acgtcagtaa
      421 ttggtgccag cagcccgcgt tcgcggcact gtttcagcaa ctggaaagta ttaccgccgc
      481 cgacaatcac gatctcagca ttttcaatag cggcaacggg atcgacaacg ctatgaatac
      541 cggtgacaga aacacccagc ggagcgagaa ccgcagccgt tttcgctgtg taatcatccc
      601 aggtctgcgt tacgccagcg aaaggaataa ataccgctga acggcgaccc tgcaactgtt
      661 cagcaatcag cggcagcgca tgttccagcc aggctttacc cggcagcgtc gagttactca
      721 ataaaagcag ttccattact tctccggtta gtaagaaatc acgggaggca tgctaccact
      781 gcgaatttaa ccacttactg acatggctca cgatgtcgca cattgcttct ggaaagtgat
      841 ttgagcaggt aacgaattca agccaatgga aataacaaat agttcgctca tgactatttt
      901 ttatcaaccc aaaggtacgg tatattccgt tttttacagg agaatttatg ctgcccgact
      961 catcagtccg tttaaataaa tacatcagcg aaagcggaat ttgctcacgc cgcgaagcgg
     1021 atcgctatat cgagcaaggc aatgtgttcc ttaatggcaa gcgagccacc attggcgatc
     1081 aggtgaaacc cggcgacgtt gtgaaagtaa acggtcagtt gattgagcct cgggaagccg
     1141 aagatttggt acttatcgcc ctgaacaagc ccgtcggtat tgtcagcacc accgaagatg
     1201 gcgagcgcga taacattgtc gatttcgtta accacagcaa acgcgtgttc ccgattggcc
     1261 gcctggataa agactcccag gggctgattt tcctcaccaa tcacggcgat ctggtgaata
     1321 agatcctccg tgcgggtaac gatcatgaga aagagtatct ggttacggtc gataaaccga
     1381 ttaccgatga atttattcgt ggtatggggg cgggggtgcc aatcctcggg acagtgacta
     1441 aaaagtgtaa agtcaaaaaa gaagcgccgt ttgtcttccg cattaccctg gtgcaggggc
     1501 tgaaccgtca gatccgtcgc atgtgcgagc actttggtta cgaagtgaaa aagctggaac
     1561 gtacgcgcat tatgaacgtc agtttaagcg gcattccgct gggtgagtgg cgcgatttaa
     1621 ctgatgatga gttaatcgac ctctttaagc tcatcgaaaa ttcctcttcc gaggcaaaac
     1681 ctaaagtgaa ggccaaaccg aaaacagcgg gcatcaaacg tccagtcgtt aagatggaaa
     1741 aaacggcgga aaaaggcggt cgcccggcgt ccaacggtaa gcgttttacc tcgccggggc
     1801 gtaaaaagaa ggggcgctga ttaacgcctc agactgatga aaacggccca aaagtgcccg
     1861 gacagtcccc tcgccccttc gggaaaaggg ttagggtgag gggaaaattg tgttcacact
     1921 gacgagtgaa aatctcaggc gttgattgtg gattagcgcc tgccgcgcgt cgacgtattt
     1981 gctgaccagc taaatgacgc ttcaggatcc ggtttataag cctgcttttt cttcaactgg
     2041 cgggcttttt tcgcttcagc ctcacgctca accattaatt tatcgatgta atctttctta
     2101 atgctgttgg tttcggagtt ggtcaatacg cgaccatgcg caatccgcgc ccgatccagc
     2161 agtgttttaa gttcacgctg ctcgcgttca gtcatctctt tttgggtaat acgggggagt
     2221 gccatgatgg tgccctcagt gagccagaga gttcagtgta cgggaattcg tttgcgagca
     2281 gaactgataa tattcatttt gccttctctt aactatttat ctctttcgat aattaattag
     2341 agatgcagat aaaaaaatta aaggcaatta ttctccgata aagacgctgg ttaacagagt
     2401 acaggctcgt ttattgatga gcatagtgac aagaaaatca atacggcccg aaatatagct
     2461 tccaggccat acagtattta ctcaaacaaa ttactatgca gtttttgcac cacctgctcg
     2521 gcatcttcgc cgggcaccag gaagcacagg ttatggctgg atgcgccgta acaaatcatg
     2581 cgaatgttga acggttccag tacgccgaat acctctttgc caacgccgca ggcttttgac
     2641 aggtcattgc caatcaacgc gaccagcgcc agaccttctt ccacttccac ccggcacagt
     2701 gcggaaagct ccatcagcag agattgcgtc agcaacgtat cgccagtgga ggttgaaccg
     2761 gtggtatcaa gggttaatgc cacgctcact tctgacgtgg tgattaagtc taccgaaata
     2821 ttatgccgcg cgaggatgcc gaaaacttct gcgaggaagc cgcgagaatg cagcatattc
     2881 aggctgtgca aagtgagtag agtctgattg cgacgaagcg ccagcgcgcg gaacagcggc
     2941 ggattttcag ttttattgca caccagcgta ccacctgcgc gtgagtcttt gctggagccg
     3001 acaaagaccg ggatatcgct gcgtactgcg gggagcaacg ttgctggatg cagtactttt
     3061 gcaccaaaag ttgccatctc tgccgcttcg gcaaacgcga tttcatcaat gcgttttgct
     3121 gcggaaacca cgcgtggatc ggtggtgtag atgcccggga cgtcggtcca gatatcaaca
     3181 cgagatgcgt gtaaagcctc cgccagcaag gctgccgtat aatcgctgcc tccacggcca
     3241 agcgtcgttg tacgaccttt attttcgcta ccgataaatc cctgggtgat cactaagccg
     3301 tcattgaggc gtgggagcag ctgcagcgcg gccagttccg ccagcgcggc tacatctggc
     3361 tctgcacgac caaatcggtc gttggtacgc atcactttac gtacatcaaa ccactgtgcc
     3421 tgaacatcgc gttcgcgcag gatctcaaca aacagcaggg tcgacatcag ctcgccgtgg
     3481 ctgaccagct catctgtcag cgccggagac gttgccagcg ccgccgcttc tgccagaaca
     3541 gtaatgttct ccagcagacg ttcaatctct tcacggataa cgttcgggta acgcagacgt
     3601 tccagaatgg caaactggat gttgcgaata gcgtcgagtt tttcgaatcg ctcgccaggt
     3661 tccagccctt ccgctaaagc gaccagcaga ttagtgatac cagcagaagc cgagaggact
     3721 actaaacgca cgttggcatc agaaagcaca atatcagcgc tgcggttcat ggcgtcaaaa
     3781 tcagctacgc tggtaccgcc aaatttggag acaacaattt cagacataac tacctcgtgt
     3841 caggggatcc attttcagcc ttggcacaag ggaagagcgg aagacgggtg ggcgcagagc
     3901 gatacttcgc tactattttc acccagaagt gctccaccac ttgcgaaacg cccgactgcg
     3961 aacgcttctg gtgacaaccc aggggattca gcccctgtag ccgatgatga acgtggccag
     4021 ccgttcaatc acctcggcga tgcaccccct caggtgttat cacaggactg gctcctccaa
     4081 caccgttact tgggcaacgc gcctcttctg gcctgcgcta gcgcaggtag tacatttata
     4141 aataaagggt gagcggggcg gttgtcaacg acggggtcat gcggattttt catccactcc
     4201 tggcggtcag tagttcagct aataaatgct tcactgcgct aagggtttac actcaacatt
     4261 acgctaacgg cactaaaacc atcacatttt tctgtgactg gcgctacaat cttccaaagt
     4321 cacaattctc aaaatcagaa gagtattgct aatgaaaaac atcaatccaa cgcagaccgc
     4381 tgcctggcag gcactacaga aacacttcga tgaaatgaaa gacgttacga tcgccgatct
     4441 ttttgctaaa gacggcgatc gtttttctaa gttctccgca acattcgacg atcagatgct
     4501 ggtggattac tccaaaaacc gcatcactga agagacgctg gcgaaattac aggatctggc
     4561 gaaagagtgc gatctggcgg gcgcgattaa gtcgatgttc tctggcgaga agatcaaccg
     4621 cactgaaaac cgcgccgtgc tgcacgtagc gctgcgtaac cgtagcaata ccccgattct
     4681 ggttgatggc aaagatgtta tgccagaagt caacgcggtg ctggagaaga tgaaaacctt
     4741 ctcggaagcg attatctccg gtgagtggaa gggttatacc ggcaaagcaa tcactgacgt
     4801 agtgaacatc gggatcggcg gttctgacct cggcccgtac atggtgaccg aagcactgcg
     4861 tccgtacaaa aatcacctga acatgcactt tgtttctaac gtcgatggga ctcacatcgc
     4921 ggaagtgctg aaaaaagtaa acccggaaac cacgctgttc ctggtagcat ctaaaacctt
     4981 caccactcag gaaaccatga ccaacgccca tagtgcgcgt gactggttcc tgaaagcggc
     5041 tggtgatgaa aaacacgttg cgaaacactt tgcggcgctt tccaccaatg ccaaagccgt
     5101 tggcgagttt ggtattgata ctgccaacat gttcgagttc tgggactggg ttggcggccg
     5161 ttactctttg tggtcagcga ttggcctgtc gattgttctc tccatcggct ttgataattt
     5221 tgttgaactg ctttctggcg cacacgcgat ggacaagcat ttctccacca cgcctgccga
     5281 gaaaaacctg cctgtactgc tggcgctgat tggcatctgg tacaacaatt tctttggtgc
     5341 ggaaactgaa gcgattctgc cgtatgacca gtatatgcac cgtttcgcgg cgtacttcca
     5401 gcagggcaat atggagtcca acggtaagta tgttgaccgt aacggtaacg ttgtggatta
     5461 ccagactggc ccgattatct ggggtgaacc aggcactaac ggtcagcacg cgttctacca
     5521 gctgatccac cagggaacca aaatggtacc gtgcgatttc atcgctccgg ctatcaccca
     5581 taacccgctc tctgatcatc accagaaact gctgtctaac ttcttcgccc agaccgaagc
     5641 gctggcgttt ggtaaatccc gcgaagtggt tgagcaggaa tatcgtgatc agggtaaaga
     5701 tccggcaacg cttgactacg tggtgccgtt caaagtattc gaaggtaacc gcccgaccaa
     5761 ctccatcctg ctgcgtgaaa tcactccgtt cagcctgggt gcgttgattg cgctgtatga
     5821 gcacaaaatc tttactcagg gcgtgatcct gaacatcttc accttcgacc agtggggcgt
     5881 ggaactgggt aaacagctgg cgaaccgtat tctgccagag ctgaaagatg ataaagaaat
     5941 cagcagccac gatagctcga ccaatggtct gattaaccgc tataaagcgt ggcgcggtta
     6001 atcatcgtcg atatgtaggc cggataaggc gttcacgccg catccggcaa ccgatgcctg
     6061 atgcgacgcg gtcgcgtctt atcaggcct
//
LOCUS       DACAPB010000229         6002 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.230, whole genome shotgun sequence.
ACCESSION   DACAPB010000229 DACAPB010000000
VERSION     DACAPB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.230"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            46..369
                     /gene="iraM"
                     /locus_tag="IT904_004219"
     CDS             46..369
                     /gene="iraM"
                     /locus_tag="IT904_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAP3193911.1"
                     /translation="MKWIVIDTVIQPTCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYHIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(472..1722)
                     /gene="icd"
                     /locus_tag="IT904_004220"
     CDS             complement(472..1722)
                     /gene="icd"
                     /locus_tag="IT904_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAP3193912.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1894..2547
                     /gene="rluE"
                     /locus_tag="IT904_004221"
     CDS             1894..2547
                     /gene="rluE"
                     /locus_tag="IT904_004221"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAP3193913.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPSIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2557..3018
                     /gene="nudJ"
                     /locus_tag="IT904_004222"
     CDS             2557..3018
                     /gene="nudJ"
                     /locus_tag="IT904_004222"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAP3193914.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3072..4178
                     /gene="mnmA"
                     /locus_tag="IT904_004223"
     CDS             3072..4178
                     /gene="mnmA"
                     /locus_tag="IT904_004223"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP3193915.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4214..4855
                     /gene="hflD"
                     /locus_tag="IT904_004224"
     CDS             4214..4855
                     /gene="hflD"
                     /locus_tag="IT904_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAP3193916.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4859..>6002
                     /gene="purB"
                     /locus_tag="IT904_004225"
     CDS             4859..>6002
                     /gene="purB"
                     /locus_tag="IT904_004225"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP3193917.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHN"
BASE COUNT         1494 a         1558 c         1475 g         1475 t
ORIGIN      
        1 catcctaatg aaccatatta aataccgtgg gataagacat aacaaatgaa gtggatagta
       61 attgacacgg taattcaacc tacatgtggt atatcttttt cagccatatg gggtaatatg
      121 aaaatgatca tctggtatca atctactata tttctccctc ctggcagtat atttacaccg
      181 gttaagtctg gtattatcct taaggataaa gaatatccta ttactattta tcacatcgca
      241 ccattcaaca aggatttatg gagtttactc aaaagcagtc aagagtgtcc tccaggagaa
      301 agcaaaataa caaataaatg tttacataat agttgcatta taaaaatatg cccatatggg
      361 ctcaagtaat gggcttcata taaatatgct cgatatgcta ttaataatat ccacataaat
      421 aaaacaacgg gcgtgttata cgcccgtttc aatatttaac acatggagag attacatgtt
      481 ttcgatgatc gcttcaccaa actctgaaca tttcagcagc ttagcgcctt ccatcagacg
      541 ttcgaagtca taagttacgg tcttggcatt gattgcgcct tccatacctt taacaatcag
      601 gtcagccgct tcagtccaac ccatatggcg cagcatcatc tcagcggaga gaataataga
      661 gccaggattt actttgtcct gaccggcata tttcggcgca gtaccgtggg tggcttcaaa
      721 cagggcgcat tcgtcaccga tgtttgcacc aggggcgata ccgataccgc caacctgcgc
      781 tgccagggcg tcagaaatgt agtcaccgtt caggttcata caggcgataa catcatattc
      841 agccggacgc agcaggatct gttgcaggaa tgcatcagca atcacgtctt taatgacgat
      901 ctctttgcca gtgttcgggt ttttaacttt cagccacggg ccaccgtcga tcagttcacc
      961 gccaaactct tcacgcgcca gctggtagcc ccagtcttta aacgctcctt cggtgaactt
     1021 catgatgttg cctttgtgca ccagagtcac agagtcacga tcgttagcaa ttgcgtattc
     1081 gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa cacggcttaa taccgatacc
     1141 acaatgttcc gggaagcgaa ttttcttcac ccccatctct tcacgcagga atttaatcac
     1201 tttctcggcg tcggcagagt ctgctttcca ttcgataccc gcataaatgt cttccgagtt
     1261 ttcacggaag ataaccatat cggtcagttc agggtgttta accgggcttg gagtgccctg
     1321 atagtaacgt accggacgca ggcagatgta gagatccagt tcctggcgca gggcaacgtt
     1381 cagagagcga ataccgccac caaccggagt ggtcagcgga cctttaatgg caacgcgata
     1441 ttcacgaatc agatcaagag tttcagcagg cagccagacg tcctgaccat aaacctgtgt
     1501 ggatttttca ccggtgtaaa tttccatcca ggagatttta cgctcgcctt tataggcttt
     1561 ctcgactgca gcgtcgacca ctttcagcat ggctggggtt acatctacac cgattccatc
     1621 accttcaatg taagggataa tcggattttc aggaacgttg agtttgccgt tttgcagggt
     1681 gatcttcttg ccttgtgccg gaacaactac tttactttcc attcacctct ccttcgagcg
     1741 ctactggttt gctcgtctgc caccacgttg catatgcgtt tgcgtcctgc gatacggatg
     1801 ctttagagca attttttgtt aatgatttgt aattggcttg tcaatactac cctattgttt
     1861 gccgtcatga aagatgcgcg ttattaggct ataatgcggc aattcataat ctctgaaaat
     1921 accatgcaaa aaacttcttt tagaaatcac caggttaagc gattcagctc gcaacgttct
     1981 accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc tgttcaataa accctacgat
     2041 gttcttccgc agttcaccga tgaagccgga cgcaaaacat taaaagaatt catcccggtt
     2101 cagggcgttt atgcagcagg tcgccttgac cgcgatagcg aagggttgct ggtgctgacc
     2161 aataacggcg cgttgcaggc gcgtttaacc cagccgggta aacgcaccgg aaaaatctat
     2221 tatgtgcagg tggaaggtat tcccacacaa gacgcacttg aagccttgcg caatggcgta
     2281 accttaaatg atggccctac cctgcccgcc ggcgcggagc tggttgacga acccgcgtgg
     2341 ttatggccgc ggaatccatc aattcgtgaa cgcaaaagta ttcccaccag ctggctgaag
     2401 atcaccttat atgaaggacg taatcgccag gtgcgccgca tgaccgccca tgttggcttc
     2461 cccacgctgc gactgattcg ctatgcgatg ggtgattact ctttggataa tcttgccaat
     2521 ggcgaatggc gagaagtgac agattaagga tgcaaaatgt ttaaaccgca cgttaccgtt
     2581 gcttgcgtgg tgcacgcaga aggcaaattt ttagtcgttg aagagacgat taatggtaaa
     2641 gcgttatgga accaacctgc cgggcatctg gaagccgatg aaaccttagt ggaagccgcc
     2701 gcccgtgaac tgtgggaaga aaccggcatc agcgcgcagc cgcaacactt tattcgtatg
     2761 catcagtgga ttgcgccaga taaaacgccg tttttgcgtt tcctctttgc cattgagctt
     2821 gagcaaatat gcccgaccca gcctcatgac agcgatatcg actgctgccg ttgggtcagc
     2881 gccgaagaaa ttttacaggc gtcaaatctt cgttcgccgc tggtggcgga aagtattcgt
     2941 tgttatcaaa gcgggcaacg ttatccgctg gagatgattg gcgattttaa ctggcctttt
     3001 acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc cgccttgaag ttcaatgtcg
     3061 tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt cggcatgtcc ggcggtgtcg
     3121 attcctccgt ttctgcctgg ctgttgcaac aacagggata tcaggtcgaa ggcctgttta
     3181 tgaagaactg ggaagaagac gacggtgagg aatattgcac agcggcagcg gatctggctg
     3241 atgcccaggc tgtctgcgac aagctcggca ttgaactgca caccgttaac tttgctgccg
     3301 agtactggga caacgtcttc gaactgttcc ttgccgaata taaagccggt cgcacgccga
     3361 atccggatat tctgtgcaac aaagagatca aatttaaagc cttcctcgaa tttgccgccg
     3421 aagatttagg tgccgattat atcgctaccg gtcattacgt acgtcgggcc gatgtcgatg
     3481 gcaagagccg cctgctgcgt ggtctggaca gcaataaaga ccagagctac ttcctttata
     3541 cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt cggcgaactg gaaaaaccgc
     3601 aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc gaagaaaaaa gactctaccg
     3661 gcatttgctt cattggcgaa cgtaaattcc gcgagttcct gggccgttat ctcccggcgc
     3721 aaccgggcaa aatcattacc gtcgatggcg atgaaattgg tgagcaccag gggctgatgt
     3781 atcacactct cggtcagcgt aaaggtctgg gtatcggtgg caccaaagaa ggtaccgaag
     3841 aaccgtggta tgtggtggac aaagacgtcg aaaacaacat tctggttgtc gctcagggcc
     3901 atgaacaccc gcggctgatg tctgtcgggt tgattgccca gcagttgcac tgggtcgatc
     3961 gcgaaccatt caccggcact atgcgttgca cggtaaaaac ccgctatcgc cagaccgata
     4021 tcccttgcac cgtcaaggcg ctggacgatg atcgcattga agtgattttc gatgaaccgg
     4081 ttgccgccgt gacgccgggc cagtctgccg tcttctataa cggtgaagtg tgcctcggtg
     4141 gcggtattat tgagcagcgt ctgccgctgc cggtctgatt attatcttta cttaaaacaa
     4201 ggaagcagtg aacgtggcaa agaattacta tgacatcacc ctcgccctgg ccggtatttg
     4261 tcagtcggca cgcctggtgc aacaactcgc tcaccagggg cattgtgatg ccgatgcgct
     4321 acacgtctca ctcaacagta ttattgatat gaaccccagc tcgacgctgg cggtttttgg
     4381 cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg ctcggcgtgc tcaatgccag
     4441 cagtcgccag ggcttaaacg ccgaattaac ccgctacaca ctcagcttga tggtgcttga
     4501 gcgcaaactc tcctcagcga aaggcgcgct cgacactctg ggcaaccgga tcaacggcct
     4561 gcaacgccag ctcgaacact tcgatttaca gtccgaaacg ctgatgagcg cgatggctgc
     4621 tatctatgtt gatgtgatta gcccgcttgg cccgcgcatt caggtcaccg gttcccctgc
     4681 tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca accctgctgg caggcattcg
     4741 cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg caactgatgt tttctcgtaa
     4801 tcgcctgacc actcaggcaa aacaaattct tgctcattta accccggagt tgtgatctat
     4861 ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga cgctacggcg ataaagtcag
     4921 cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa ttccgtgtac aagttgaagt
     4981 acgttggctg caaaaactgg ccgcgcacgc agcgatcaag gaagttcctg cttttgctgc
     5041 cgacgcaatc ggttaccttg atgcaatcgt cgccagtttc agcgaagaag atgcggcgcg
     5101 catcaaaact atcgagcgta ccactaacca cgacgttaaa gcggttgagt atttcctgaa
     5161 agaaaaagtg gcggagatcc cggaactgca cgcggtttct gaattcatcc actttgcctg
     5221 tacttcggaa gatatcaata acctctccca cgcattaatg ctgaaaaccg cgcgtgatga
     5281 agtgatcctg ccatactggc gtcaactgat tgatggcatt aaagatctcg ccgttcagta
     5341 tcgcgatatc ccgctgctgt ctcgtaccca cggtcagcca gccacgccgt caaccatcgg
     5401 taaagagatg gcaaacgtcg cctaccgtat ggagcgccag taccgccagc ttaaccaggt
     5461 ggagatcctc ggcaaaatca acggcgcggt cggtaactat aacgcccaca tcgccgctta
     5521 cccggaagtt gactggcatc agttcagcga agagttcgtc acctcgctgg gtattcagtg
     5581 gaacccgtac accacccaga tcgaaccgca cgactacatt gccgaactgt ttgattgcgt
     5641 tgcgcgcttc aacactattc tgatcgactt tgaccgtgac gtctggggtt atatcgccct
     5701 taaccacttc aaacagaaaa ccattgctgg tgagattggt tcttccacca tgccgcataa
     5761 agttaacccg atcgacttcg aaaactccga agggaatctg ggcctttcca acgcggtatt
     5821 gcagcatctg gcaagcaaac tgccggtttc ccgctggcag cgtgacctga ccgactctac
     5881 cgtgctgcgt aacctcggcg tgggtatcgg ttatgccttg attgcatatc aatccaccct
     5941 gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg ctggatgaac tggatcacaa
     6001 ct
//
LOCUS       DACAPB010000230         5942 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.231, whole genome shotgun sequence.
ACCESSION   DACAPB010000230 DACAPB010000000
VERSION     DACAPB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.231"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            208..927
                     /locus_tag="IT904_004226"
     CDS             208..927
                     /locus_tag="IT904_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3193918.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVPGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTYETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIIEFQVKYFFRDKSRRTEC"
     gene            complement(950..1849)
                     /gene="eamA"
                     /locus_tag="IT904_004227"
     CDS             complement(950..1849)
                     /gene="eamA"
                     /locus_tag="IT904_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAP3193919.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2044..3231
                     /gene="ydeE"
                     /locus_tag="IT904_004228"
     CDS             2044..3231
                     /gene="ydeE"
                     /locus_tag="IT904_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAP3193920.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGSTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVILPMNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3358..3453
                     /gene="mgtS"
                     /locus_tag="IT904_004229"
     CDS             3358..3453
                     /gene="mgtS"
                     /locus_tag="IT904_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAP3193921.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3672..4562)
                     /gene="dgcZ"
                     /locus_tag="IT904_004230"
     CDS             complement(3672..4562)
                     /gene="dgcZ"
                     /locus_tag="IT904_004230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAP3193922.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLFSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLASYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4817..5209)
                     /gene="ydeI"
                     /locus_tag="IT904_004231"
     CDS             complement(4817..5209)
                     /gene="ydeI"
                     /locus_tag="IT904_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAP3193923.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            5484..>5942
                     /locus_tag="IT904_004232"
     CDS             5484..>5942
                     /locus_tag="IT904_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAP3193924.1"
                     /translation="MNINKDKNVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGS
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGSEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCE"
BASE COUNT         1662 a         1405 c         1262 g         1613 t
ORIGIN      
        1 ctttttcata ttttccctta ctggtatata acagactaca ttgtgtaaat atcatatccc
       61 gcagtagcta tacatcctgg tacattgatc acagtaatac agtaataaag ttgttcaaca
      121 tcagggtaat aattaaacga ctcggccata cgataaacca atcaggactc aactccttgt
      181 tttcaatggc tcttaaaagg tgcaaaagtg atttttcaaa agattgcccg cttacttaaa
      241 tctgagatca atggctattc atggcacgtt ggtgatttgt tgccgtcaga agctgaactc
      301 gcagttcgtt ataatgtttc gcgtaatacc ctgcgtaagg cattatcctt gctggaaggc
      361 gaaggaatta ttcacagaaa gcatggttca ggaacataca ttcaaaaaaa gaattttgtt
      421 gcacacattg atcacatgaa cagcttcagt gaaattgcgc ataaaagcgg caaagaggca
      481 gggagtcaga ttatgaaatt tgaagtgcag gatgcttcac ccactattgc aactgagctg
      541 aatttagtgc ccggtgagca ggtttattac ataaagagac tgcgattcat tgaggataat
      601 gcggcacaat tagaagaaac gtggatgtca gtggcacgtt ttcctgattt aaccgtatca
      661 catatgcaaa aatccaaatt ttcgtatatt gagaacgaat gcgggatcaa aatcattggc
      721 acctatgaaa ctttctcccc tacttttcct accccagaaa tcgccagtat tttacggatc
      781 agcccacggg atcccatact taaaattcag acccaggctg tggacagtaa ctctattccg
      841 ctggattatt cgttacttta cagcaatatt atcgagttcc aggtaaagta ctttttccgc
      901 gataaaagca gacgcacaga gtgctgacat cacattctgt gcgtatggtt tagcctctaa
      961 cctttaccgc tttacgccac cgcaagccaa atacattgat atacagcccg gtcataatga
     1021 gcaccgcacc taaaaattgc agacccgtta agcgttcatc caacaatagt gccgcacttg
     1081 ccagtccgac tacgggcacc agtaacgata acggtgcaac ccgccaggtt tcatagcgtc
     1141 ccagtaacgt cccccagatc ccataaccaa caattgtcgc cacaaacgcc agatacatca
     1201 gcgacaagat ggtggtcata tcaatagtaa ccagactgtg aatcatggat gcggaaccat
     1261 cgagaatcag cgaggcaaca aagaagggaa tgattgggat taaagcgctc cagattacca
     1321 gcgacatcac cgccggacgc gttgagtgcg acatgatctt tttattgaag atgttgccac
     1381 acgcccaact aaatgctgca gccagggtca acataaagcc gagcatcgcc acatgctgac
     1441 cgttcagact atcttcgatt aacaccagta cgccaaaaat cgctaaggcg atccccgcca
     1501 attgtttgcc atgcagtcgc tccccgaaag taaacgcgcc aagcacgata gtaaaaaacg
     1561 cctgtgcctg taacaccagc gaagccagtc cagcaggcat accaaagtta atggcgcaga
     1621 acagaaaggc aaactgcgca aaactgatgg ttaatccata ccccaagagc aaattcagtg
     1681 gtactttcgg tcgtgcaaca aaaaagatgg ccggaaaagc gaccagcata aagcgcaaac
     1741 cggccagcat cagcggtggc atgttatgaa gccccacttt gatgaccaca aaatttagcc
     1801 cccatacgac cactaccagt agcgccaaca caccatcttt tcgcgacatt ctaccgcctc
     1861 tgaatttcat cttttgtaag caatcaactt agctgaattt acttttcttt aacagttgat
     1921 tcgttagtcg ccggttacga cggcattaat gcgcaaataa gtcgctatac ttcggatttt
     1981 tgccatgcta tttctttaca tctctaaaac aaaacataac gaaacgcact gccggacaga
     2041 caaatgaact tatccctacg acgctctacc agcgcccttc ttgcctcgtc gttgttatta
     2101 accatcggac gcggcgctac gctgccattt atgaccattt acttgagtcg ccagtacagc
     2161 ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc tcactattgg cgtcgttttt
     2221 agcctcggtt ttggtatcct ggcggataag ttcgacaaga aacgctatat gttactggca
     2281 attaccgcct tcgccagcgg ttttattgcc attcctttag tgaataacgt gacgctggtt
     2341 gtgctctttt ttgccctcat taactgcgcc tattctgttt ttgctaccgt gctgaaagcc
     2401 tggtttgccg acaatctttc gtccaccagc aaaacgaaaa tcttctcaat caactacacc
     2461 atgctaaaca ttggctccac catcggtccg ccgctcggca cgctgttggt gatgcagagc
     2521 atcaatctgc ccttctggct ggcagctatc tgttccgcgt ttcccatgct tttcattcaa
     2581 atttgggtaa agcgcagcga gaaaatcatc gccacggaaa caggcagtgt ctggtcgccg
     2641 aaagttttat tacaagataa agcactgttg tggtttacct gctctggttt tctggcttct
     2701 tttgtaagcg gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat tgctgatggg
     2761 gattttgccg aaaaggtggt cgcggttatt cttccgatga atgctgccat ggtggttacg
     2821 ttgcaatatt ccgtgggccg ccgacttaac ccggctaaca tccgcgcgct gatgacagca
     2881 ggcaccctct gtttcgtcat cggtctggtc ggttttattt tttccggcaa cagcctgcta
     2941 ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc gccgggcgag
     3001 tatatgttga ttgaccatat tgcgccgcca ggaatgaaag ccagctattt ttccgcccag
     3061 tctttaggct ggcttggtgc cgcgattaac ccattagtga gtggcgtagt gctaaccagc
     3121 ctgccgcctt cctcgctgtt tgtcatctta gcgttggtga tcattgctgc gtgggtgctg
     3181 atgttaaaag ggattcgagc aagaccgtgg gggcagcccg cgctttgttg atttaagtcg
     3241 aacacaataa agatttaatt cagccttcgt ttaggttacc tctgctaata tctttctcat
     3301 tgagatgaaa attaaggtaa gcgaggaaac acaccacacc ataaacggag gcaaataatg
     3361 ctgggtaata tgaatgtttt tatggccgta ctgggaataa ttttattttc tggttttctg
     3421 gccgcgtatt tcagccacaa atgggatgac taatgaacgg agataatccc tcacctaacc
     3481 ggccccttgt tacagttgtg tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc
     3541 cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag
     3601 gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc tcaatgaatg
     3661 ttaaacggag cttaaactcg gttaatcaca ttttgttcgt caataaacat gcagcggttt
     3721 cttccggttt gcttaccctc atacattgcc cggtctgctc ttccaatgac cacatccaga
     3781 ggctcttcag gaaatgcacg actgacacct gctgtcacgg taatgttgat atgcccttca
     3841 gaatgtgtga tggcatggtt atcgactaac tggcaaattc tgacacctgc acgacatgct
     3901 tcttcatcat tagccgcttt gacaataatg ataaattctt cgcccccgta gcgataaacc
     3961 gtttcgtaat cacgcgtcca actggctaag taacttgcca gggtgcgtaa tactacatcg
     4021 ccgattaaat gcccgtaggt atcattaacc aatttaaatc ggtcaatatc caacaacatt
     4081 aaataaagat tcagaggctc agcgttgcgt aactgatgat caaaggattc atcaagaacc
     4141 cgacgccccg gcaatcccgt caaaacatcc atattgctac ggatcgtcag caaataaatt
     4201 ttgtaatcgg ttaatgccgc agtaaaagaa aacaacccct cctgaaaggc gtcgaaatgc
     4261 gcgtcctgcc agtgattttc aacaatagcc agcattaatt cccgaccaca gttatgcata
     4321 tgttgatggg cagaatccat tagccgaacg taaggtaatt catcgttatc gagtggcccc
     4381 agatgatcaa tccaccgacc aaactggcac agtccataag aatggttatc cgttatttct
     4441 ggcttactgg catctctcgc gaccacgctg tgaaacatac tcaccagcca ctggtagtgg
     4501 gcatcgatag ccttattgag atttaacaag atggcatcaa tttccgttgt cttcttgatc
     4561 attgccactc ctttttcaca gttccttgtg cgcgctattc taacgagaga aaagcaaaat
     4621 tacgtcaata ttttcataga aatccgaagt tatgagtcat ctctgagata acattgtgat
     4681 ttaaaacaaa atcagcggat aaaaaagtgt ttaattctgt aaatcacctc tgcattatcg
     4741 taaataaaag gatgacaaat agcataaccc aataccctaa tggcccagta gttcaggcca
     4801 tcaggctaat ttatttttat ttctgcaaat gagtgacccg aacgacggcc ggcgcgcttt
     4861 tcttatccag actgccgcta atgttgatca tctggtccgg ctgaacttct cgtccatcaa
     4921 agacggccgc aggaataacg acattaattt caccgctctt atcgcgaaaa acgtaacggt
     4981 cctctccttt gtgagaaatc aaattaccgc gtagtgaaac cgaagcgcca tcgtgcatgt
     5041 tttttgcgaa atcaacggtc attttttttg catcatcggt tccgcgatag ccatcttcta
     5101 ttgcatgagg cggcggtggc gctgcatcct gttttaaacc gccctggtca tctgccaacg
     5161 cataaggcat gacaagaaaa cttgctaata caatggcctg aaatttcata ctaactcctt
     5221 aattgcgttt ggtttgactt attaagtctg gttgctattt ttataattgc caaataagaa
     5281 tactgccaat tgttataagg catttaaaat cagcccacta gctgtcaaat attcagagaa
     5341 tttaactcac taaggttaag aagattgaag agtcttaaac atattttcag aataatcgga
     5401 tttatatgtt tgaaaattat tatattggac gagcatacag aaaagcaaat cacctttaca
     5461 tataatagcg tggacaaaaa acagtgaaca ttaataaaga taaaaatgta caactcgcag
     5521 ataccgacac tattgaaaac ctgacatccg cgttgagtca aagacttatc gcggatcaat
     5581 tacgcttaac taccgccgaa tcatgcaccg gcagtaagtt ggctagcgcc ctgtgtgcag
     5641 ctgaagatac acccaaattt tacggtgcag gctttgttac tttcaccgat caggcaaaga
     5701 tgaaaatcct cagcgtaagc cagcaatctc ttgaacgata ttctgcggtg agtgagaaag
     5761 tggcagcaga aatggcaaca ggtgccatag agcgtgcgga tgctgatgtc agtattgcca
     5821 ttaccggcta cggcggatcg gagggcggtg aagatggtac gccagcgggt accgtctggt
     5881 tcgcgtggca tattaaaggc cagacctaca ctgcggttat gcattttgct ggcgactgcg
     5941 aa
//
LOCUS       DACAPB010000231         5930 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.232, whole genome shotgun sequence.
ACCESSION   DACAPB010000231 DACAPB010000000
VERSION     DACAPB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.232"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..549
                     /gene="feoB"
                     /locus_tag="IT904_004233"
     CDS             <1..549
                     /gene="feoB"
                     /locus_tag="IT904_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="cytoplasmic membrane ferrous uptake system
                     permease; mutations disrupt the ability of Escherichia
                     coli to take up ferrous iron; GTP-binding protein which
                     requires GTP for efficient iron(II) uptake; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP3193925.1"
                     /translation="YTAENIQDEEFNPAEFNLGEELFSAVDETWQSLKDTFSLSVLMN
                     PIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMGAIARESSRGWM
                     GFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIGLLRRARSRVDI
                     ELLATRKSVSSCCAASTTGDCH"
     gene            549..785
                     /gene="feoC"
                     /locus_tag="IT904_004234"
     CDS             549..785
                     /gene="feoC"
                     /locus_tag="IT904_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAP3193926.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            988..1866
                     /gene="rpnA"
                     /locus_tag="IT904_004235"
     CDS             988..1866
                     /gene="rpnA"
                     /locus_tag="IT904_004235"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAP3193927.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(1895..2665)
                     /gene="bioH"
                     /locus_tag="IT904_004236"
     CDS             complement(1895..2665)
                     /gene="bioH"
                     /locus_tag="IT904_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAP3193928.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            2703..3386
                     /gene="gntX"
                     /locus_tag="IT904_004237"
     CDS             2703..3386
                     /gene="gntX"
                     /locus_tag="IT904_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAP3193929.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            3445..4020
                     /gene="nfuA"
                     /locus_tag="IT904_004238"
     CDS             3445..4020
                     /gene="nfuA"
                     /locus_tag="IT904_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAP3193930.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            4381..5697
                     /gene="gntT"
                     /locus_tag="IT904_004239"
     CDS             4381..5697
                     /gene="gntT"
                     /locus_tag="IT904_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP3193931.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(5742..>5930)
                     /locus_tag="IT904_004240"
     CDS             complement(5742..>5930)
                     /locus_tag="IT904_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAP3193932.1"
                     /translation="LGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGV
                     NKLLKDLDRRRRAAAKKK"
BASE COUNT         1365 a         1564 c         1536 g         1465 t
ORIGIN      
        1 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cagaatttaa cctcggtgaa
       61 gagctgttca gcgcggtaga tgaaacctgg cagagcctga aagacacctt cagccttagc
      121 gtactgatga atcccattga agcgagcaaa ggcgacggcg aaatgggtac cggggcgatg
      181 ggcgtgatgg atcagaaatt cggtagcgca gctgccgcat acagctacct gattttcgtc
      241 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
      301 tggatgggct tctccatcct gtgggggctg aatatcgctt actcactggc aacattgttc
      361 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggta
      421 atcctgttta acatcgtagt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
      481 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
      541 tgccattaat ggcttcactt attcaggtac gtgatttgct ggcgttacgg ggccgtatgg
      601 aagcgaccca gataagccag acattgaaca ctccgcagcc aatgattaac gccatgctgc
      661 aacagctgga aagcatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
      721 ctggcagttg taaaagctgc ccggaaggca aagcctgtct gcgcgagtgg tgggcgctgc
      781 gttaaccata ctccatcgcc tgttttgaaa agcccggtat gcgtttgcat ccgggctttt
      841 ttgcgtgcgg ctttccataa aaatgcaatt cttgcagcgc gacgtaagtt cctctgaaag
      901 cgtctcgcag ggatgaaaac tggctaatgc acagatgtgg agtggcgcgt agagtcgcgg
      961 cattcaaaca acaggtgaag gaacgccatg agtaaaaagc agagttccac cccacatgat
     1021 gcgctgttca aactcttttt acgccaaccg gagacggcac gcgattttct tgcgtttcat
     1081 ttaccggcac ccattcacgc gctctgtgat atgaaaaccc tcaagctgga gtcgagcagc
     1141 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     1201 caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     1261 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     1321 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     1381 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca actttatgcc
     1441 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga
     1501 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgatct gatggggctg
     1561 gtagagcaaa tggcctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     1621 ctgtttaatt acatactgca aactggcgac gctgtacgtt ttaacgattt tatcgacggc
     1681 gttgccgaac gttcaccgaa acacaaggag agtttaatga ctattgcgga aagattgcgg
     1741 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     1801 cctcttacag acatcatgcg ctttaccggg gtgtcagaag aagagttggc tgcggcgagc
     1861 cagtaaattt ctgtctcgcc atttcaaaag ttgcctacac cctctgcttc aacgccacca
     1921 gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga
     1981 tatatgattc gctgtgaggc caaagtttat ccagcattgg caccactttg cgcggcacca
     2041 gaccgtcgag atagccatac aatcgcaaaa acggcatgga cacgttttgc agcggctgac
     2101 ggagatcgac cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg
     2161 gtaacgccag tacggttttc ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca
     2221 tggtttgtaa cgccaggaac cgctccactg tacgctgaaa atcatcactg agttgctgct
     2281 gaaatcccgc cagcacgtcc ggttttatcc ccggccactc gtcacgagca ctaaaacaag
     2341 gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac gcaatctggc
     2401 ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgttgca
     2461 gcacggcttc ggccatatca gcaagtgaca gcgcaccaaa tccccggcta cgcccgaagc
     2521 cgggcaggtc aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc
     2581 acacttcggc attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt
     2641 tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac
     2701 atatgctaac agtaccggga ttatgctggc tatgccgaat gccactggcg ttaggtcatt
     2761 gggggatttg ttcggtctgt tcacgcgcca cccgcacaga taaaacgtta tgcccacaat
     2821 gtggattacc cgccacacac tcccatcttc cctgcggtcg ctgcctgcaa aaaccgccgc
     2881 cctggcaaag actggtcaca gttgccgact atgcgccgcc gttaagtctg ctcatccacc
     2941 agcttaaatt ttcccggcgc agtgaaatcg ccagcgccct gtcacgtctg ttactattgg
     3001 aagtcttgca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc
     3061 cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc
     3121 cgttatcacg ctggttgcac tgccaatggg atagcgaagc cgtcacacgt acacgggcca
     3181 ctgcgaccca gcattttctc agtgcccggc tgcgcaagcg caacctgaaa aatgcctttc
     3241 gtcttgaatt gcccgtgcaa ggtcgccata tggtgattgt ggatgatgtc gttaccaccg
     3301 gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg
     3361 tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg gcgtattata accaactaaa
     3421 atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt
     3481 gccaaactgc tggcaaatca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct
     3541 ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt ggaagccacc
     3601 gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgcaccatac
     3661 ctggaagatg cagagatcga ctttgttacc gaccagttgg gttctcagtt aacgctgaaa
     3721 gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cgctgatgga gcgcgtggag
     3781 tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggtcg cgtttcgctg
     3841 atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt
     3901 tccatggtgg atgtgacgct gaaagaaggg atcgagaagc agctgctaaa cgaattcccg
     3961 gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa
     4021 gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacat
     4081 tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa atccctccct tcgtttcccc
     4141 gcgataatat gaccaacctc tcataattta aatttacccc gctctggtga ttctcaaacg
     4201 ccagatgtta cccgtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa
     4261 taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata
     4321 attagagttt gtcgtcagaa aattgacgtt acccataaca aatgaaaggc caggtaaatc
     4381 atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgcttc
     4441 aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaagga
     4501 atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc
     4561 cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa tgctggcaga ctgcggtggc
     4621 gcacaacgta tcgccaccac gctgattgcc aaatttggta aaaaacacat ccagtgggcg
     4681 gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg ctttgtgctg
     4741 atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct gtatgttggt
     4801 gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg
     4861 actgcgattg ccaccatttt caatgccgat atgggtaaaa ccctgctgta cggtactatt
     4921 ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt gaaaggtatc
     4981 gataagccaa ttccggaagg tctgtacagc gcgaaaacct tcagcgaaga agagatgccg
     5041 agctttggcg tcagtgtctg gacctctctg gtgccggtag tactgatggc gatgcgtgcg
     5101 attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt
     5161 gacccggtaa tggcaacgct gattgccgtg ctgattgcga tgttcacctt tggtctaaac
     5221 cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg
     5281 atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc
     5341 gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc
     5401 tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact
     5461 gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga gctgatggtt
     5521 attgcggttg gttccggtag tgtgattttc tctcatgtga acgatccggg cttctggctg
     5581 ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc gatgctggaa
     5641 accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa
     5701 cactgactgc cggatgcatc gcatccggca tcaaccgcac tctacttctt cttcgctgca
     5761 gctctgcgcc gtctgtccaa atccttcagc aacttgttca cgccatcatc ggcaaacatc
     5821 gtctcaagcg ttgcggagag cttgcgccgc cagtttttat actggtaact ggtgccagga
     5881 atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa
//
LOCUS       DACAPB010000232         5909 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.233, whole genome shotgun sequence.
ACCESSION   DACAPB010000232 DACAPB010000000
VERSION     DACAPB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.233"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            53..1456
                     /locus_tag="IT904_004241"
     CDS             53..1456
                     /locus_tag="IT904_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP3193933.1"
                     /translation="MGDKVKARQLAKRVGTPLVAGTQDPVNSVVDVMKFAEQYGLPLV
                     IKAAYGGGGRGMKVVWRREDIPELYVSAVREAVTAFGRGECFIERFLDRPRHIEAQVL
                     ADKYGNVQVLGTRDCSLQRRNQKLIEEAPAPFLSDEQRQRIHDAARELCREAAYCGAG
                     TVEFLLSPEGEFSFLEVNTRLQVEHTVTEETTGIDLVIAQLEIARGKRLDNTETPPPH
                     GHAFEFRINAEDPARGFLPVSGTISDFSPPSGIGIRLDSGVRTGSTVPPTFDSLLAKL
                     IVWAPSREMALARARRALAEFKIEGLASVLPFHRAALQEPDFITAPFSVHTRWIETDF
                     AVDLPLADRTLPAREESLTCVWIELDGKRVALGLPDSLLNGVTQPDAQPARPLRVNAG
                     VVEAPISGVLLSWHVEESQQVSEGDPIAMMEAMKMETQLYAPCAGRITLRAAAGEMVT
                     AGTMLADIHRDTDPMAS"
     gene            complement(1497..2333)
                     /locus_tag="IT904_004242"
     CDS             complement(1497..2333)
                     /locus_tag="IT904_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HAP3193934.1"
                     /translation="MANYPAAYQSTSGSALDVDRDFYKALAEGPRELITSHLIPIRTG
                     FAWEVPAGHLFRITTPEGPQVGDLNIWNRRDPRERMWVSRTRQLQRAHLSTYDRLWSN
                     LPFIRPIATITADSLADYGIDSDGGRVHDLMGTRCDPYVNKLLTGEDFDFHCHSNLVR
                     AVQPWGLTEFDVHDVMNVFQCTGLNDNDQYFMKACPAKKGDYLEFFAEIDLLCALSTC
                     PGGDLSLPMWGEEAQDTLRVCRPLGVEIYKPQADLVANWTPPVSPDYRGNHGMQLKPV
                     AW"
     gene            complement(2333..3055)
                     /locus_tag="IT904_004243"
     CDS             complement(2333..3055)
                     /locus_tag="IT904_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013509091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP3193935.1"
                     /translation="MKGSAGESGALFIEIPEHIDLTGLPAFERISILLRENIISGNLV
                     AGQALGEIELAALCDSSRNTLREALRFLHGEGLVNYHQNRGVFVRQLDKRDIRDIFKA
                     RRHLEVLALTVNTPISDFHLARMEKHLDNAAQSAAASEWRSVGTHSLRFHQSIVQMLG
                     CERFNDFFSVLLAQLRLLFCSGAGERDFQLPWIARDRQILALLNQQQSEAACRALIDY
                     LDYSERQLTTNFSHPITYGESY"
     gene            complement(3065..4573)
                     /locus_tag="IT904_004244"
     CDS             complement(3065..4573)
                     /locus_tag="IT904_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HAP3193936.1"
                     /translation="MHFDWRYLISLFHYADFWRASWLVVQLSVMTWLGGIVLGLGVAL
                     AKQSRNTLLRNAAGCYIWLFRSLPLLVLLIFIFNLPQIFPTTSGLLSNPFAAGLLALI
                     LSETAYIAEIHRGGLLSVQRGQFEAGRALGLNNTQIRLSIIIPQALRISLPTLVNEFI
                     TIVKMTSLVSVISLSEILMVGERLYTDNFKVMETLLAVAFYYVLIVTLFERLFGWLER
                     RWDVLNRKPAPFDVAQARASLPVQPVATRKSHSASAKPILKLTGLSKSFNGKPVLNNI
                     NLSVKPGEVISIIGPSGSGKTTLIRMINGLEKMDQGSVELLGRAFIHAGQRENALPYR
                     AQITRLGMVFQGFNLFPHKTVLENLMLAPRLHNLDNPNDLYKRSLAMLDKVGMLEHAT
                     KYPHQLSGGQQQRVAIARTLVMKPAVVLFDEPTSALDPERVSEVLQVIEKLAEEGITM
                     LIVTHEMRFAFTISDRVVFMEQGRIEIDAPPEEIRQMRNHRILRFIDDMATA"
     gene            complement(4598..5413)
                     /locus_tag="IT904_004245"
     CDS             complement(4598..5413)
                     /locus_tag="IT904_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP3193937.1"
                     /translation="MKTMLRIACLAAPVMLNVAQASTLQPGNLIIGSDLTYPPYNFLK
                     QDKPAGFDTEFIELIASPLKLKPVVKDTRFASLILGLKSNKFDVIASTLYVTPERAQQ
                     VDFLPYMKTGGSLLVIAGSAFQPQKPEDLCGKRVASIKGGAWIARLNKVSTDYCQSKS
                     LGAISVQEFPTSPEATQALLSAAVDVQYEDTAVAKATLEKTGQRLKISSQQMIYPVVV
                     GLAVNKDNPELLKELKASLAQRVSDGSYRALLQKYNLQMPGADEISKALAGSL"
BASE COUNT         1416 a         1561 c         1568 g         1364 t
ORIGIN      
        1 cattaccgcg ggattgtgct ggattggccc ttcgccagaa gccattgata tattgggcga
       61 caaagtgaaa gcacgtcagc tggcaaaacg tgtcggcacg ccgttagtgg caggaactca
      121 ggatccggtt aacagcgtag ttgacgtcat gaaatttgcc gagcaatatg gcctgccgct
      181 ggtgatcaaa gccgcttatg gtggtggcgg acggggaatg aaagtggtat ggcgaagaga
      241 ggacattcca gaactgtatg tctcagccgt gcgcgaagcg gtgaccgcat tcggccgtgg
      301 cgaatgtttt attgaacggt ttttagacag gccgcgtcac attgaagcgc aggtgctggc
      361 agacaaatat ggcaacgtac aggtgctagg cacgcgcgat tgttcgttac agcgccgtaa
      421 ccaaaagctg attgaagaag cgcccgcgcc ttttctcagt gatgaacaac gtcagcgtat
      481 tcatgatgca gccagagagt tgtgtcgcga agcagcgtac tgcggcgctg gtaccgtcga
      541 gtttctgctt agcccggaag gggagttctc cttccttgag gtcaataccc gcctgcaggt
      601 tgaacacact gtgacggaag aaaccactgg cattgacctt gtcatcgccc aactggagat
      661 cgcccggggc aaacggctgg acaataccga aactccgcct ccgcacggcc atgcttttga
      721 gtttcgtatc aatgccgaag atcctgcgcg tggatttctt cctgtttccg gcacgatcag
      781 tgacttttca ccgccgtccg gcattggtat ccgtctggac agcggagtcc ggaccggttc
      841 gacagtgccg cccacttttg attctctgct ggcaaaactt atcgtctggg cgccatcgcg
      901 cgaaatggcg cttgcgcgag ctcgacgcgc tcttgctgaa ttcaaaatag aagggctggc
      961 aagcgttctt cctttccacc gcgctgccct gcaggagccg gatttcatta ccgcgccttt
     1021 ttctgtgcat acacgctgga ttgaaactga ttttgctgtc gatttgccgc tggcagaccg
     1081 gacattgcct gcacgggaag aaagcctgac gtgtgtctgg atagaactcg atggaaagcg
     1141 tgttgccctg gggctgccgg attcgctgct gaatggcgtt acacagccgg atgcgcagcc
     1201 cgcacggccc ttgcgggtga acgcaggcgt ggtagaagcc cccatttcgg gtgttttact
     1261 gagctggcat gtcgaagaaa gccagcaggt tagcgaaggc gaccccatcg ccatgatgga
     1321 agcgatgaaa atggaaacgc agctctatgc gccctgcgct ggtcgaatta ctcttcgggc
     1381 cgctgctggt gagatggtga cggcagggac aatgctggcc gacatacaca gagataccga
     1441 tcccatggcg tcgtgatgat aaaaaaaccc gctttatcag cgggtttttt atggtattac
     1501 caggcaactg gcttcagttg cataccgtga ttaccacggt agtccggtga aaccggcggc
     1561 gtccagttgg caaccaggtc cgcctgcggc ttataaattt ccaccccaag cggacggcag
     1621 accctcagcg tgtcttgcgc ctcttcgccc cacatcggta gcgacaaatc gccgcccgga
     1681 caagttgaaa gtgcacataa cagatcgatt tcggcgaaaa actcaaggta atcacccttc
     1741 ttcgccgggc aggctttcat aaaatactgg tcattgtcat tcaggccggt gcactggaat
     1801 acattcatca cgtcatggac atcaaattct gtcagtcccc agggctgtac agcgcgtacc
     1861 aggttggaat ggcaatggaa atcgaaatct tcgccagtca gtaatttatt tacataggga
     1921 tcgcaacgtg ttcccatcag gtcatgtacg cggccgccgt cgctgtcgat accataatcc
     1981 gccagcgaat ctgcggtgat ggtcgctatc ggacgaataa acggcaaatt cgaccagagt
     2041 cgatcgtaag tcgacaggtg cgcccgttgt aattggcggg tacgggatac ccacattctt
     2101 tcgcgtgggt cacggcggtt ccagatattc aaatccccta cctgcggtcc ttccggcgtg
     2161 gtaatccgga atagatgtcc ggcgggaact tcccaggcaa agccagtacg aattggaatc
     2221 agatgagagg tgataagctc acgcgggcct tcggcaagcg ctttgtagaa atcgcggtca
     2281 acgtctaacg ccgagccgga cgtgctctga tacgctgcag gataattcgc catcagtaag
     2341 attctccata ggtaattggg tgactaaagt tggtcgttaa ctgtcgttct gagtaatcga
     2401 gatagtcgat tagcgcacgg caggctgcct cgctttgttg ctggttcagt aaggccagga
     2461 tttggcgatc gcgtgcgatc catggcaact ggaaatctcg ctccccggcg ccgctgcaaa
     2521 acaacagacg caactgcgcg agtaacacac tgaaaaaatc attaaaacgc tcgcatccca
     2581 gcatctgtac gatgctctga tgaaatcgca ggctgtgcgt accgacgctg cgccattccg
     2641 acgccgccgc tgattgcgca gcgttatcca gatgcttttc catccgcgcc agatgaaaat
     2701 cgctgatagg cgtgttgacc gttaacgcca gtacttccag atggcggcga gctttgaata
     2761 tgtcgcggat atcgcgctta tcaagctgac gaacaaagac cccgcgattc tggtgataat
     2821 tcaccagtcc ttcgccatgc agaaaacgca atgcctcacg tagggtgtta cgcgagctat
     2881 cgcataatgc cgccagttca atttcgccca gcgcctgccc agctaccaga ttgccgctga
     2941 tgatgttttc acgcagcagg atggatatac gctcaaatgc aggcagcccg gttaaatcaa
     3001 tatgttcagg aatttcaatg aacaatgcgc ctgattcccc ggcagatcct ttcatacaat
     3061 ctcctcaggc cgtagccata tcgtcgataa agcggaggat ccggtgattg cgcatttggc
     3121 gaatttcttc cggcggcgca tcaatttcga tacgcccctg ctccataaac accacccggt
     3181 cggaaatggt gaacgcgaac ctcatctcgt gggttacaat cagcatggtg atcccctctt
     3241 ccgccagctt ttcaatcacc tgtagtactt cgctgactcg ctccggatcg agtgccgagg
     3301 ttggctcatc aaaaagcacc actgcgggct tcattaccag agtacgggcg atggccacgc
     3361 gctgttgctg tccgcctgat aattgatggg ggtatttcgt agcgtgttcc agcatgccaa
     3421 ctttatcaag catggccagg ctgcgcttat acagatcatt agggttatcg aggttatgca
     3481 gtcgcggggc cagcatcagg ttttccagca ctgtcttatg gggaaacagg ttgaagccct
     3541 gaaaaaccat tccaagccgg gttatctggg cacggtaagg gagtgcgttt tcgcgctgtc
     3601 ctgcatgaat aaatgcacgt cccagcagct caacactacc ctgatccatt ttttccagac
     3661 cattgatcat gcggatcaac gtggtttttc ctgagcccga cgggccaata atggagatca
     3721 cctcaccagg tttcacgctg aggttgatat tgttcagtac cggcttgcca ttgaaggact
     3781 tactgagacc agttaacttc aaaatcggtt ttgccgatgc gctgtggctt ttgcgcgtgg
     3841 caacgggctg tactggcaag ctggcgcgag cctgcgccac atcaaacggt gcgggcttac
     3901 gattcagcac atcccagcgg cgctccagcc agccaaacag gcgctcgaac aacgtaacga
     3961 tcagcacata atagaaggcc accgccagca gggtctccat caccttgaag ttgtcagtat
     4021 aaaggcgttc acccaccatc aggatttctg acagcgagat aaccgacacc agcgaagtca
     4081 ttttcacgat ggtgataaat tcattcacca acgttggtag agagatacgt aatgcctgcg
     4141 gaataataat gcttagccgg atctgggtat tattgagccc cagcgcccgt cctgcttcaa
     4201 actgcccgcg ctgaactgac aacaatccac cacgatggat ttccgcaata taggcggttt
     4261 cgctaagaat cagcgccagc aaaccggctg cgaatggatt cgacagtaac ccgctggttg
     4321 tcgggaaaat ttgtggcagg ttaaaaataa agatcagtaa caccagcaac ggcaggctgc
     4381 ggaataacca gatgtagcag cctgccgcat ttcgcaacag ggtgttacgc gattgtttag
     4441 ccagcgccac gcctaagccc agcacaattc ccccgagcca cgtcatcacg ctcagttgca
     4501 ctaccagcca actggcacgc cagaaatctg cgtagtggaa taaacttatc aggtaacgcc
     4561 agtcaaaatg catgtttcct ccgggggatg ataaccgtca aagactacct gccagcgctt
     4621 tcgatatttc atcggcaccc ggcatttgca ggttgtactt ctgcaacagc gcacggtagc
     4681 tgccatcgct caccctctgc gccaaggatg ctttcagttc cttcaataat tcagggttgt
     4741 ctttattgac cgccagcccc accaccacgg gatagatcat ttgttgagag gaaattttca
     4801 ggcgctgccc ggttttctct aatgtcgctt ttgcaactgc ggtatcctca tattgcacat
     4861 caaccgccgc cgacagcagg gcctgagttg cttctggcga ggtcgggaat tcttgcactg
     4921 atatggcgcc aaggctttta gactggcagt agtcggtaga caccttattc aggcgggcaa
     4981 tccaggctcc gcctttaatt gatgccaccc gtttgccgca caggtcttcg ggcttctgcg
     5041 gctggaaagc cgagccggct atcaccaata aagagccgcc ggttttcata taaggcagaa
     5101 aatccacctg ctgcgcgcgc tccggcgtga catagagtgt tgaagcgata acatcgaact
     5161 tattgctttt taagccaagg atcaggctgg cgaaacgcgt gtctttaacg accggtttca
     5221 gctttaacgg ggaggcaatt aactcaataa actcagtatc aaaacccgcc ggtttgtcct
     5281 gcttaaggaa attataaggc ggataagtca ggtcagagcc aatgatcagg ttacccggtt
     5341 gcagtgtgga cgcctgcgcc acattcaaca ttacaggtgc agcaagacag gcaatgcgta
     5401 acatcgtttt catagtggag tctctgtggt tgtttgttca acaatgttta agcaccaatc
     5461 atgccaatta ataaattacc aagactgagc ggctgcgggg tatgttgtgg ggattcggcg
     5521 tggtttatat tgcgcaatga aggggcaagg tacgaaaaaa tgcaccaaaa tgaggagtct
     5581 ggttgagggt gttctctggt ttattctgaa cccgcccaca attagtggac ccgctactaa
     5641 gtgagtaaac tcttaaccag aggcgattac tcatgacaaa accatcataa acctgcaaaa
     5701 cggcccgtaa acagtacacg ccggaatttc gcgatgaagc actgagacta gctgaactca
     5761 cagcacaaag ctgacgttaa aggtctacag catgggaaac ttccggatgc cgcgtcagcg
     5821 gcatggaggc gccagatgag gttacatgtg tctgtggagt gaaatggcgc cgaaagcccg
     5881 gcgtagccgg ttaccgggct ataaattct
//
LOCUS       DACAPB010000233         5882 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.234, whole genome shotgun sequence.
ACCESSION   DACAPB010000233 DACAPB010000000
VERSION     DACAPB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.234"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..475
                     /locus_tag="IT904_004246"
     CDS             <1..475
                     /locus_tag="IT904_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193938.1"
                     /translation="TTFNGRYYGDHTGGTAVRLTSQGEAYIGDHFIVANAIVYSFGND
                     IYSYETGAHSDFESIRAVVRPAYIWDQYNQTGVELGYFTQQNKDANSNKFNESGYKTT
                     LFHTFKVNTSMLTSRPEIRFYATYIKALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            502..1671
                     /locus_tag="IT904_004247"
     CDS             502..1671
                     /locus_tag="IT904_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP3193939.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            1686..2408
                     /locus_tag="IT904_004248"
     CDS             1686..2408
                     /locus_tag="IT904_004248"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP3193940.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(2470..3057)
                     /locus_tag="IT904_004249"
     CDS             complement(2470..3057)
                     /locus_tag="IT904_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193941.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(3106..3579)
                     /locus_tag="IT904_004250"
     CDS             complement(3106..3579)
                     /locus_tag="IT904_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAP3193942.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(3646..4311)
                     /gene="yieH"
                     /locus_tag="IT904_004251"
     CDS             complement(3646..4311)
                     /gene="yieH"
                     /locus_tag="IT904_004251"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAP3193943.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4478..5815
                     /locus_tag="IT904_004252"
     CDS             4478..5815
                     /locus_tag="IT904_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP3193944.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIDLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1485 a         1403 c         1440 g         1554 t
ORIGIN      
        1 taccaccttt aatggtcgtt attatggtga tcacaccggc ggaacagcgg tacgtctgac
       61 ttcgcagggc gaagcctata ttggcgatca tttcattgta gctaacgcga ttgtttactc
      121 cttcggtaac gatatatata gctacgaaac aggcgcccac tctgatttcg aatctattcg
      181 tgcggttgtt cgcccggcct atatttggga ccaatataac cagacaggtg ttgaactggg
      241 ctatttcacc cagcaaaaca aagatgcgaa tagtaataaa tttaatgagt ctggttataa
      301 aaccacgctc ttccatacct ttaaagtcaa taccagtatg ttgacctcgc gtccggaaat
      361 tcgtttctac gccacgtata tcaaagccct ggaaaacgaa ctggatggct tcacctttga
      421 agacaataaa gacgaccagt ttgctgtcgg tgcccaggct gaaatctggt ggtaatggaa
      481 cttaatgaaa ggaatattac gatgaatata aaaattgctg ctttaacgct ggctattgcc
      541 agcggtattt ctgctcagtg ggccatagca gcggatatgc cagccagccc ggcacccact
      601 attccggtta aacagtatgt gactcaggtc aatgccgata acagcgtgac ctttcgctac
      661 tttgcccctg gggcaaaaaa cgtctccgta gtggtgggtg ttccggttcc ggacaatatt
      721 cacccgatga ccaaagacga agcgggagtc tggtcgtggc gcacacctgt cctgaaaggc
      781 aatctgtacg aatatttttt caatgttgat ggtgtacgca gcattgatac tggcaccgca
      841 atgactaagc ctcagcgcca ggttaactcc agtatgattc tggtgccagg cagttatctg
      901 gatacgcgtt ctgttgcgca tggtgatttg atcgccataa cttaccactc caacgcattg
      961 caatctgaac gtcagatgta tgtctggacc ccgccaggat acaccggcat gggcgagcct
     1021 ttgccagtgc tctatttcta tcacggcttt ggtgatactg gacgttccgc tatcgatcag
     1081 gggcgtatcc cgcaaatcat ggataacctg ctggctgaag ggaaaattaa accgatgctg
     1141 gtggtgatcc cagataccga aaccgatgcg aagggcatta ttcccgaaga tttcgtgcct
     1201 caggaaagac gtaaagtctt ttatccgctg aatgctaaag cggcagatcg cgaactgatg
     1261 aacgatatta tcccgctgat tagcaagcgt tttaacgtcc gtaaagatgc cgatggtcgc
     1321 gcactggcag ggctttcaca aggtgggtat caggcgctgg tatcgggaat gaatcatctg
     1381 gaaagctttg gctggctggc cacattcagt ggtgttacca cgacaaccgt accggatgaa
     1441 ggtgtcgcgg cccggctgaa cgatccggca gctattaacc agcaactacg taattttact
     1501 gtggtcgttg gtgataaaga tgtcgtaacc ggcaaggata tcgccgggct gaaaactgag
     1561 cttgagcaga aaaaaattaa gtttgattac caggaatatc ccggcctgaa ccatgaaatg
     1621 gatgtctggc ggcctgccta tgcagccttc gtacagaaat tatttaaata aaataaggca
     1681 ctaagatgaa attaatcatt accgaagatt accaggaaat gagccgtgtc gcggcacacc
     1741 atctgcttgg ttatatgtcg aagacgcgtc gtgttaacct ggcaattacc gccggtagca
     1801 cgcccaaagg catgtatgaa tacctcacca ccctggttaa aggtaagccc tggtacgata
     1861 actgctattt ctataatttc gatgaaattc cctttcgcgg caaagaggga gaaggtgtaa
     1921 cgattaccaa tctgcgtaat ctgtttttca cccctgcggg gatcaaagaa gagaatatcc
     1981 agaagctcac tattgataac taccgcgagc atgatcagaa actggcgcgt gaaggcggcc
     2041 tggatttggt ggtgctggga ttaggtgcag atggtcattt ttgtggcaat ttgccgaata
     2101 cgacccattt ccatgagcaa accgtggagt tcccgatcca gggagagatg gttgatattg
     2161 tggcgcatgg tgagttgggc ggcgactttt cgttagtgcc tgacagctac gtgacgatgg
     2221 ggccgaaaag catcatggcg gcgaaaaact tgctgataat tgtcagtggc gcaggtaaag
     2281 cgcaggcact gaaaaatgtt ctgcaaggtc cggttaccga agatgttcca gcttccgtgt
     2341 tgcagctgca tccttcgctg atggtaatcg ctgataaagc cgctgccgca gaattagcgc
     2401 tcggttaagc tgaagaaata aggccggatg tatgaattac gctccggcct gttctcatta
     2461 tttaaataac tacgaaaaat caccccacag tctcagcttg ccgcagtgga gacagcggaa
     2521 gagataaccc tgacaatcac cacccttttg caggcattta gctagatcag aatttctaat
     2581 accgaaattc tcacaatctt cttcaaggtt ggcaaattca tcgaggcgat cttttatatc
     2641 attccagccc acatagccga taaaagcaca gaaatcgcca caatgcgcaa gccagaattc
     2701 ctgttgccat ccatgataac ctggcgtgcg ttcaaccaac tctttcagca tttcatcagg
     2761 atatgtgttc ttaataccgg caaattcgtc ctcttcatca tactcaaatt caacaccttc
     2821 tatgctggca tcatcctgaa aactacctgc aaacttttca gcagcagaac cgtccgcaat
     2881 acaccacgga cagagatgtt caacttcatc aacgcaataa aagggacccg agtaataaac
     2941 tgacgtctgt tgttcacagc aatcgcactc tacggtttta tcctgttcaa atgcgcctgt
     3001 ttccagtggc ttgggatgat atttgaattg gggtaacggc ctgatatttt gagtcatagt
     3061 aacatcctgt ctgcttttga gtagcccaaa gataaagtat cccgcttaac ttaaagagtc
     3121 tgtttcagga catgaaatat ccaatccggg cagacgggta atgattctgc tcatcaccac
     3181 acctgccagc aatgcaggaa tgatgcggac aacgagactg tcaccagaaa gaatgttttc
     3241 aaacaactcc gaggaagcca tgcccttaat atgtacaata actgcacaat agatctcggt
     3301 gataacgacg ccacctatgc caccagccag acaacgataa tttttctgtg acccgatctt
     3361 ttccggcaga caggcgacca tcacgccggt taacaatgca ggaatagctc ccgtagtaat
     3421 cgacaataag agaaacaatg gaagtacctg tattattacc aaaagaacca gcggttcttt
     3481 tgcgaagaat atgtagagga caagaaacag aacaccaatt aacggtccta aaaccgcaaa
     3541 ataaagtccg tgatgtatta cccgacgtga aacagacata tcaaacaccc ttaacttacc
     3601 tttgagcgcc aatttaagcg gggtaagtga agggagtgtg aagaactatg ccgtaatatc
     3661 ccaaccacgc gctttccaca gttccggtaa ctgcgaaaga tgggtaaagg tggtgacttt
     3721 cgggtgaacg atcggcttat tgtgcgggtc ggcgcagaag tagaacactt ccatacctgc
     3781 gtcgatacca gattgcgcac cggcaactga gtcatcaacc agaatgcagt tttctacatt
     3841 tacattcatc gcttttgccg catggaacat taacgccggg tctggcttcc agcgctgaat
     3901 atcgtagccg ctgaacagtt tatccgggaa gtagtgcaac atattcagct tgcccataga
     3961 atgctgcatt ttgttatttg ggccgttaga taccacacac attggcgcag tgatcgctga
     4021 caggagcgct ccagcccctt cgatggcttc cagttctgaa tcgaacagcc gagcgacttc
     4081 tgcacggtaa acgtgttcag cttctgtttt cgctaacgta acaccatgtt caagggaaac
     4141 aatatcgata atttcgtaca gttttacacc cttgaaacgt ttgaatacct cttcaggatc
     4201 gagcgtaata ccaaattcct gaaacatcgt tacatatgcg cgagagcaaa tgacctcgct
     4261 atcgaccagc gtaccgtcgc agtcgaaaaa taccgcttct atctgggaca tgtctttcct
     4321 ttatcgttag tttaacgttt tcgtcatgcc aattcgcatg acgcaatcgt tgccgtataa
     4381 gcaaatacaa tgaaaaaaag ttatcttcag ccgtccatat tgtctcattt tggtatacga
     4441 tagcgacgga ttttccctcc ttgttcggaa atagataatg agtcaacaac acacaaccca
     4501 ggcttctggc caggggatgc tggaacgcgt gtttaaactg cgcgaacatg gcacgacggc
     4561 acggaccgaa gtgatcgccg gttttaccac cttcctgacg atggtttaca tcgtttttgt
     4621 taacccgcaa attcttggcg ttgctggcat ggataccagc gccgtcttcg tcactacctg
     4681 tctgatcgct gcattcggca gtattatgat gggactgttt gctaacctgc cagttgcact
     4741 ggcacccgct atgggcctga atgcgttctt cgcttttgtc gttgtacagg cgatgggctt
     4801 gccgtggcag gtcgggatgg gcgcaatctt ctggggcgcg atagatctgc tgttactgac
     4861 gattttccgc gttcgctact ggatgatagc caacattccg gtgagtctgc gtgtgggtat
     4921 taccagcggt atcggtctgt tcattggcat gatggggctg aaaaacgcag gtgtgattgt
     4981 cgctaacccg gaaacgctgg tgagcatcgg taatctgact tctcacagcg tacttctggg
     5041 tatcctcggc ttcttcatca ttgctattct ggcctcgcgc aacattcacg cagcggtgct
     5101 ggtttctatc gtggtgacga cgctgctggg ctggatgctg ggtgatgtgc actacaatgg
     5161 catcgtttct gcgccgccga gcgtaatgac agttgtgggt catgtagatt tagccgggtc
     5221 gtttaacctc gggctggcag gggtgatttt ctctttcatg ttggtcaact tgtttgactc
     5281 ctccggtacg ctgattggcg tgaccgataa agcaggtctg gcggatgaga aggggaaatt
     5341 cccgcgcatg aagcaggcgc tgtatgtcga cagtatctct tccgtgaccg gttcgtttat
     5401 cggtacttct tccgttacgg cttatattga gtcctcttcc ggcgtatcgg ttggcggtcg
     5461 taccggtctg acggcagtgg ttgttggtct gctgttcctg ctggttatct ttctgtcgcc
     5521 gctggcggga atggtgccag gctacgctgc agctggcgcg ttgatctacg ttggcgtgtt
     5581 gatgacctca agtcttgctc gcgtgaactg gcaggatctt actgaatctg ttccggcgtt
     5641 tattaccgcc gtgatgatgc cgttcagctt ttcgattacc gaaggtattg cgctgggctt
     5701 tatctcctac tgcgtgatga agattggtac cgggcgtctg cgtgacctta gcccgtgcgt
     5761 aatcatcgtt gcgctgctgt ttatcctgaa gattgtattt atcgacgctc attaaaatcg
     5821 atagcgcatt aaaaacccgc catgcatgat tactggcggg ttttttattt agatcttaac
     5881 tc
//
LOCUS       DACAPB010000234         5758 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.235, whole genome shotgun sequence.
ACCESSION   DACAPB010000234 DACAPB010000000
VERSION     DACAPB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.235"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..574
                     /gene="fbp"
                     /locus_tag="IT904_004253"
     CDS             <1..574
                     /gene="fbp"
                     /locus_tag="IT904_004253"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAP3193945.1"
                     /translation="EEDFLQPGNKQVAAGYVVYGSSTMLVYTTGCGVHAFTYDPSLGV
                     FCLCQERMRFPEKGKTYSINEGNYIKFPNGVKKYIKFCQEEDKSTNRPYTSRYIGSLV
                     ADFHRNLLKGGIYLYPSTASHPDGKLRLLYECNPMAFLAEQAGGKASDGKERILDIIP
                     ETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(607..1602)
                     /gene="yjfF"
                     /locus_tag="IT904_004254"
     CDS             complement(607..1602)
                     /gene="yjfF"
                     /locus_tag="IT904_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAP3193946.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLISEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVDLDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(1589..2611)
                     /locus_tag="IT904_004255"
     CDS             complement(1589..2611)
                     /locus_tag="IT904_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP3193947.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(2625..4127)
                     /locus_tag="IT904_004256"
     CDS             complement(2625..4127)
                     /locus_tag="IT904_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP3193948.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEDQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFALLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFIDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTVLIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(4267..5223)
                     /gene="ytfQ"
                     /locus_tag="IT904_004257"
     CDS             complement(4267..5223)
                     /gene="ytfQ"
                     /locus_tag="IT904_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAP3193949.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            5533..>5758
                     /locus_tag="IT904_004258"
     CDS             5533..>5758
                     /locus_tag="IT904_004258"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134479.1"
                     /note="catalyzes the hydrolysis of pyrophosphate to
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HAP3193950.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVP"
BASE COUNT         1401 a         1599 c         1460 g         1298 t
ORIGIN      
        1 ggaagaagat ttcctccagc ctggtaacaa acaggttgcg gcaggttacg tggtatacgg
       61 ctcctctacc atgctggttt acaccaccgg atgcggtgtt cacgccttta cttacgatcc
      121 ttcgctgggc gttttctgcc tgtgccagga acggatgcgc ttcccggaga aaggcaaaac
      181 ctactccatc aacgaaggaa actacattaa gtttccgaac ggggtgaaga agtacattaa
      241 attctgccag gaagaagata aatccaccaa ccgcccgtat acctcacgtt atatcggttc
      301 actggtcgcg gatttccacc gtaacctgct gaaaggcggt atttatctct acccaagcac
      361 cgccagccac ccggacggca aactgcgttt gctttatgag tgcaacccga tggcattcct
      421 ggcggaacaa gcgggcggta aagcgagcga tggcaaagag cgtattctgg atatcatccc
      481 ggaaaccctg caccagcgcc gttcgttctt tgtcggcaac gaccatatgg ttgaagatgt
      541 cgaacgcttt atccgtgagt tcccggacgc gtaatctcct gcgcctctcc acacggagag
      601 gcgtttttat cgctgcgcaa tgttgactct tgtcactggc gaactctgac gattctccca
      661 cagcaccgtt aatccacgct gtaatgctat aaaaataaac aacaaaatac cgatggcgat
      721 tttcgtccac catgagctca gcgtgccatc aaagtttatg taagtctgaa tcagtccctg
      781 aatcgccacg ccaaaaagcg tccctaatac cgttccaacg ccaccgctca aaagcgtacc
      841 gccaatcacc accgacgcga tggcgtccag atccacacct acgcccgcca gcgcatatcc
      901 ggcctgggta taaatcgaga agacaatacc cgccagcgtt gccagtccgg tggagagcat
      961 ataaatgcga atagtggtgc tgcgagtgga aatccccatc aggttcgccg acgttgcgtt
     1021 gccgccaatg gcgtatacct gattaccaaa gcgggtgcga tgcgcgagga aaataccgat
     1081 aaccaccacc gccagcatca gcagccccat cgcacttaag cgaccgccgc cggggatttt
     1141 ccatgcaagg cttgaaagcg tgtcataaac aggatggttt atcgggatcg actcttctga
     1201 gatgagatag ctgacaccgc gcaaaaagaa catccccgcc agggtaatga taaatgccgg
     1261 gatcttcagg gcgtcgatca aaagccccat aaatgcgccg aaggcacagc ccatcaccag
     1321 cactagcgga aacgccagca gcggcgagag gccgaaatcg ccaatcactt ttgccagaaa
     1381 cacgccagta aaggcgatca ccgaaccgac ggagagatcg atcccaccgg agaggatcac
     1441 aaaggtcatg ccaacggcaa tgatcccaag aaaggcgtta tcggtcagga tattgcagat
     1501 cactcttgtg gaagcaaaac cgggaaactg ggtcaggcag taaagataac ccaacacaaa
     1561 gacgccgatg gtgatcatca gtggcaaatt acgttttatc acggctacgt actcctttaa
     1621 tcagactgat aaagcgttgc gactggacaa tcagcacgca aagaaccacc accgctttta
     1681 caacctggtt catctctggc ggaaagcccg aaagcaaaat tccggtgttc atcccctgaa
     1741 taatcagcgc ccccaccacc gaaagcagta ggttaaaacg tccgcccatc agcgatccgc
     1801 cgccaataac caccgcgaga atggcgtcca gctccagcca taacccggcg ttattggcat
     1861 cggcaccgcg aatatccgcc gccacgataa tgcccgcaat cgccgcacac agcccgctca
     1921 acacgtaggt aagcatgacg atgatccgcg tgtttacccc ggcatttttt gccgcccgaa
     1981 tgttgatacc aacggcttcg ataaacatcc ccagcgccgt tttgcgggtc aacagccaga
     2041 acaggataag cgtcagcacc gcgataatga ccggcgttgg caggaacaac agcgatccac
     2101 tgccaaacca tgagagatcc ggcgagttaa acgtgacgat ctgtccggag gtgatcagtt
     2161 gcgccacgcc gcgcccggcg accatcagga tcagggtggc aacaaacggc tgaattttga
     2221 ggatcgctac cagtatgccg ttccacaatc ccgccaggat gccagtgccc agggcgctta
     2281 acaaaacaat cggcaggctg aatcccgcga ccgtcatcgc agccgttgtg gctccggcga
     2341 tagccatcac cgcccccacg gagagatcaa tcccacctgt tgcgatcacc agcgtcattc
     2401 cgatagccaa cagcgcaacg ggggccgcac ggttaagaat gtcgataggg ctaccgaaca
     2461 aacgcccatc ctgaagcact acctgccaga aatgcggggc taccaggctg tcgaccagca
     2521 gcaccagcaa tagtgctacc agctgcggca tccccgtcgg ccagcgaaag cgcctttttg
     2581 gcgtagtggt gtccggaaga gattgaggca tcacactgtt ctccttacgc cgcaatggcg
     2641 ttcatgatcg ccggaacgga aagctctgcc agcgggatct ccgccacctg tttgcgatcg
     2701 cgcatgatga tcacccggtc ggcatagccc accagctctt ccagttcaga ggagatcacc
     2761 agcagcgcca gaccatcggc gcatagcgtt tcaatcaggc ggatgatctc ggcgtgggca
     2821 ccaacatcaa tgccgcgggt tggctcatca aggatcagaa attgcggtcg ggtcagtagc
     2881 caacgtgaaa gcaacacttt ttgctgattg ccgccagaga gaaactcaat cggttgttca
     2941 gttgaaggtg tgcgaatgcc aagctggcgg ataaagcgtt cggcaatctc ttgctgctct
     3001 ttgcgggaaa tgggtcgtag ccagccgcgc tgggcctgga gagcgaggat gatattttcc
     3061 cgcaccgagg cggcagcgat gatgccatcg gttttcctgt cttccgggca gaagccgata
     3121 cccagtaccg aagcctgatg tggcgatcgc agggtttgcg gtttgccttt gatcaacacc
     3181 gtgccgctgt cagcaggttt gataccgaag atcacttcgg cggtttcggt acgtcctgat
     3241 cccagcaagc ctgccaggcc gacgatctcg ccggggcgca cttcgagatc aaacggtgct
     3301 atcgttcctt ttttgccgta atttttgaac gcggcaacgg gtttgtcgct caacaatgtt
     3361 cgcccggcac gctgtagcgc gtgggtatcc agctcgcgcc ccagcatcat ttttaccagt
     3421 tcgatctgcg gtagctcgcg cgtttcccga caacctacga aactgccgtt gcgtaagacg
     3481 gtgatccgat cgctgacctg atagacctga tcgataaagt gagtgacaaa gatcaggctg
     3541 acgccgcgat cacgcaactg acgcatcagg ccaaacagta actctacttc ctgggtgtcg
     3601 agactggcgg tgggttcatc gaggatcagc actttggcag agagatcgat agcccggcaa
     3661 atggcgacga tttgctgcat cgcgactgaa aagcggttga gcggttcgcg cacgtcgagg
     3721 gagaaaccgt aagatgccat cagttcggtg gcgcgctttt ccatctcttt acggcgtaga
     3781 agcgcgaaac gtttgggttc gcgacctatg aatagattat cagcgaccga catattgggt
     3841 agcaggttga cttcctgata aacggttccg atgccgagtt gttgtgcatg ggcggtattt
     3901 ttcggtgaga tagcctggtc ttccagccag atggtgccgc gatcggcgtg gtaaacaccg
     3961 gttaatgctt tgattagcgt cgattttccc gccccgtttt caccgagcag cgccatgatt
     4021 tcaccacggc gcaggctgaa atcaacgttg tctaacgctt taacgccggg gaaaaatttg
     4081 cttaatcctt cggtgcggag gatttcctgg tgttggtcgg tcgtcatggt ttcccccggt
     4141 gtaaagcgtg ctcccagtag gcatgataag acgcggtaag cgtcgcatca ggcttcagtg
     4201 caccatcgcc ggatgcggcg cgagcgcctt atccggccta cgaatcggca tcaggcatag
     4261 caaccctcaa taccccatat ttttcttctt ctctaactct tcttttgcgg tatcaggcag
     4321 ataaagggtg gatttggtca gcgttagctt ttcaggcatg gtgccgtctt ttttgtattt
     4381 ctccagcgcg tcgaaagcag ggcctgccat gttcggcgtc agttcaacac tggcgttcgc
     4441 ttcgccatca atcatcgctt tgtagatatc cggtacgccg tcgatagaac ctgtcagaat
     4501 atctttgcct ggtttcaggc ccgcttcttt aattgcctga attgcaccaa tcaccatgtc
     4561 gtcgttatgg gcgtaaacca tgcagatgtt tttgccgttg ttttccgctt tgataaagct
     4621 ctccatgact tctttgcctt tactgcgggt gaagtcacct gactgcgagc ggataatttt
     4681 gatatttggc gcattcttaa tggcttcggc aaagcctttc ttacggtcaa tggcgacgct
     4741 ggccccaaca gtgccctgca gctccaccac gttgcatggt ttgccattca cttcttttac
     4801 cagccagtca ccaatcaact tgccttcgag aatgttgtcg gcagtgacgg tggtcatata
     4861 gagagatttg tctttcacat caatagaacg atcgagcaag aagaccggga tttcggcatc
     4921 tttcgcctct tttaataccg gttcccaacc agtcgcaacc accggagcga taaagatcgc
     4981 atccacccct tgcgcgacga aggaacgtac cgctttaatc tggttttcct gcttttgctg
     5041 accatcggca attttcagcg taattccgcg tttttcggcc tcacttttcg ccacattggt
     5101 ttctgcggcg cgccagccag attccgatcc gacctgcgaa aatcctacgg ttaatggagc
     5161 ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta agcgtttcca
     5221 catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc gaaaaaacta
     5281 tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt aaataaaacg
     5341 ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta cggttatgga
     5401 ttttcctgct ctgtataccg tcttaaaact ggtgaaaaag gaaaatgaag acgaaaacaa
     5461 gcgaagacat tcggcgcgag ttggctataa tactcggcac ttgtttgcca catattttta
     5521 aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc ggaagacatc
     5581 tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat cgacaaagag
     5641 agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc gtgcaactac
     5701 ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact ggtcccga
//
LOCUS       DACAPB010000235         5752 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.236, whole genome shotgun sequence.
ACCESSION   DACAPB010000235 DACAPB010000000
VERSION     DACAPB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.236"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1946)
                     /gene="hrpA"
                     /locus_tag="IT904_004259"
     CDS             complement(<1..1946)
                     /gene="hrpA"
                     /locus_tag="IT904_004259"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAP3193951.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESHGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGAR"
     gene            2147..2752
                     /gene="azoR"
                     /locus_tag="IT904_004260"
     CDS             2147..2752
                     /gene="azoR"
                     /locus_tag="IT904_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP3193952.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVSA"
     gene            complement(2806..4122)
                     /locus_tag="IT904_004261"
     CDS             complement(2806..4122)
                     /locus_tag="IT904_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAP3193953.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWSCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(4112..>5752)
                     /locus_tag="IT904_004262"
     CDS             complement(4112..>5752)
                     /locus_tag="IT904_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAP3193954.1"
                     /translation="HRGHEHSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPS
                     LARSVRDVDEFVRFAASDSQVGLEEVVVIAQSVGAVLVATWIHDYAPAIRGLVLASPA
                     FKVKLYVPLARPALALWHRLRGLFFINSYVKGRYLTHDRQRGASFNNDPLITRAIAVN
                     ILLDLYKTSERIIRDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPG
                     FYHDTLGEENRALAFEKMQSFISRLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPL
                     SPVDLAYRFMRKAMKLFGTHSSGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDK
                     IYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPA
                     VSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGL
                     YELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRV
                     RSQGEMDSLVRDAGFDKCTQRIDEWGIFTVSMAVRRDN"
BASE COUNT         1453 a         1579 c         1391 g         1329 t
ORIGIN      
        1 ttacgtgcgc cggtatattc ttgtttatcg gcatctttca tgccgatatg ggaaagcaaa
       61 ccggtcagca acgcaatgtg aatttcgcga tactccgccg gttcgctgtt aaccggaatg
      121 ccaagttctt tcaccacctg acgcaactgg gtgtagatat cctgccattc gcgcacgcgc
      181 agatagttga gataatcgat acgacacagg cgacggaagg cgttggaaga aagcgccttt
      241 tgctgctcgc caagataatt ccacagattc acaaacgcga gaaagtcaga ctctttgtcg
      301 tggaagcgac gatgtttttc gtccgatgcc tgctgtttgt ccatcggacg ttcgcgcgga
      361 tcctgaatgg agagcgcgga cgtgataatc atcgcctcac gcacgcagcc atgtttttgc
      421 gcttccagca ccatacgcgc cagacgtggg tcgacaggca actgcgagag ctggcgaccg
      481 agcggcgtca gtttataggc gctggcctgt tcatcagtgg tgatcgcgcc cagctcttcg
      541 agcagacgca cgccatcctg gatattgcgt ttatccggtg cttcgacaaa cgggaacgca
      601 gcgatatcgc ccagccccag cgcggtcatc tgcaaaataa ccgaggccag gttggtacgc
      661 agaatctccg gatcggtaaa ctccgggcgc gagaggaaat cgtcttcgga atagagacga
      721 atacagatcc cttcggacac acgaccacag cggcctttac gctgattggc agacgcctgg
      781 gaaatcggct caatcggcaa acgctgcact ttggtgcgat agctgtagcg gctgatacgc
      841 gctgtaccgg ggtcgataac gtatttaatc cccggtacgg tcagcgacgt ttccgcgacg
      901 ttggtcgcca gcacaatgcg ccgtccgctg tgcgactgga ataccctatt ttgttcgctg
      961 ttcgaaagcc gcgcataaag cggcaagatt tcggtatggc gtaagttcag cttgttcagc
     1021 gcatcggcgg tatcgcggat ttcccgctcg ccgctcataa agatcagaat gtcgccatgg
     1081 ctttcctgac tcagttcgtc tacggcgtca aaaatcgcct gcaactgatc gcgctcggtg
     1141 tcatcggctt cttcaacaat cgggcgatag cgcacttcca ccggataggt ccgaccggag
     1201 acttcaataa tcggcgcatt attaaagtgg cgcgaaaagc gttccgggtc gatagtcgcg
     1261 gaagtgataa tgatttttag gtcaggacgc cgcggcagca actctttcaa atagccgagc
     1321 aaaaaatcga tattcaggct gcgttcgtgc gcttcgtcaa taatgatagt gtcgtactgc
     1381 atcagcaggc ggtcttgctg gatctccgcc agcaggatac cgtcggtcat cagcttgacc
     1441 atcgtgttat cacttacgtg atcgctgaaa cgcactttgt aaccgatgca accgcccggc
     1501 tccgttttca gctcttccgc aatacggttc gccactgttc ttgccgccag acgacgcggc
     1561 tgggtatggc cgatcagtcc tttaatcccg cgccccagct ccatacagat tttcggtaac
     1621 tgagtcgttt taccagaacc cgtttccccg gcgacgatca ccacctggtg atcacgaatc
     1681 gcttcgagaa tgtcctgttt tttctgacta accggtaaat tgtcaggata agtaatttcc
     1741 ggtcgtgccg cttcacgcag caggactttc cctgccgcct ggtcaatctc tttcgccatc
     1801 tcctggaaaa tggcctgttg tgcatcagga tttttaacct tcttcacgcc gtgcagacgg
     1861 cgagaaaaac gcagtctgtc acgcagcatc agcgaatcca gccgctgctg caaggccgta
     1921 aaggtcaatt tttgttgttc tgtcataacg ttagtgggca gtagtctgcc tgattaattt
     1981 tcttttaaat gatagatata gagtaccaca tcgcggcttt ctatgcgttg ttcaataaat
     2041 tcgaacatag gcttcgattt attgcgctat ctctgtggca ggattgtgaa taaagtgtca
     2101 acaagcaacg gggcatcgcc cattcaaaca tctataagga aacaccatga gcaaggtatt
     2161 agttcttaaa tccagcatcc tggcagggta ctctcagtct aatcagttgt ccgattattt
     2221 tgttgaacaa tggcgcgaaa agcactccgc tgatgaaatc accgttcgcg acctggctgc
     2281 aaatccgatt ccggtactgg atggcgaact ggttggcgct ctgcgtccga gcgatgcgcc
     2341 gctgactccg cgtcagcagg aagctctggc actttccgat gagttgattg ccgagctgaa
     2401 agcccacgac gttatcgtta ttgcggcacc gatgtataac ttcaacatct cgactcagtt
     2461 gaaaaactat tttgacctgg ttgcccgcgc aggcgttact ttccgctata ccgagaacgg
     2521 tccggaaggt ctggtaacgg gtaaaaaagc catcgttatt accagccgcg gcgggatcca
     2581 caaagatgga ccaacggacc tggtgacgcc gtatctgtcc acgttcctcg gctttatcgg
     2641 cattaccgat gtgaaatttg tcttcgccga agggatcgca tacggtccgg aaatggcagc
     2701 gaaagcacag tctgacgcaa aagcagccat cgacagcatt gtttctgcat aaccggacac
     2761 ggtcttgatg tggactgtag atattcagtc cacatctcaa tccacttacc tgttttccca
     2821 taatctcagc atcgctttgt gctcgtctgt cagcacaatc tgcgggcgtc tggctcgaat
     2881 atagctaatc gcttcattaa cggttttaca gtgtccgtaa cataacaacc atgccgccac
     2941 caccagcgca ctgcgcgata atcccaatgc acaatggacc agaacgctgc cttgctcttc
     3001 gcgtaatgtt tccagcatcg ccacggcctg tcggagctcc ccctcttccg gaaccaccag
     3061 atccagcatc ggtacacaaa aatagagtcg atcttttgtg gctcgtccgc gagggaattc
     3121 aaaggtgacg tccagaaccg cattctgtgc cggaatatgt cgtggaaacg cccctaaata
     3181 aacaccagca gtaattttgc tcaccggctc caggcgaaga caagaccaac gcatagacag
     3241 ccacatcccg atacgccagg gcaatgtcag ccagtaaacg gcgggcggga gtttcccctg
     3301 actatctttc cctgttgtta tcgcgccaag cccaccgtac ccacggccaa tgatgaatag
     3361 cgataatact ggccaacata accagactga ccaccataac tgaagcatta tcattagctc
     3421 catcaacaca atgcacgagc ccgcgcctac gacatatggc agtgctattt tgattcgacg
     3481 ttgatcaggt ttctgataat tccaacgacg gtcgacgggc accatccagt caattaacat
     3541 acctaccgcc agccccgtga tgacatcaat aaaatgatgc tgccaggtcg tcagcgtcga
     3601 aatggcgatg agtaaaaacc atccgccgca gactttacgc cacctcacag ccagatgctg
     3661 acgaaagtga cgccaaagta gccagcagag aataatatgc agcgaaggag actggttata
     3721 aggcagatca aacagttcaa gttgcgaaaa tagccatcct gtcaccccac tcacttcagg
     3781 acggataaaa ctaaacttca gcgggtagag aaaaaaaccg cagcaggcca ttaccgttgc
     3841 cagaataagc cggtggacaa gtcggcgctg ttcgaatgtc gtgctacaaa cgaacagcga
     3901 aaatccatat aaaagatcca gactccagta aggaacaata gtccagggaa gaaaagggat
     3961 cgccgtttcc caaccgaata cctgactggg aatatcatgg ctgttaaggt cctgaaccgc
     4021 ggtgaactga ttaagagatc cataggtgaa gaagaaaaac ggggccagca acaataacca
     4081 gccagcgcct tgtagcaata cgttccgacg ttcagttatc acgacgcacc gccatcgaaa
     4141 ccgtaaaaat accccactca tcaatccgtt gtgtgcattt atcaaatccg gcatcacgca
     4201 cgagtgaatc catctcccct tgcgaacgca cgcgcattac ccacggttta ccatctttat
     4261 gactggttaa caccccggca atcatctcca gttgagggtg ccacggctgc ccggtgtaga
     4321 tgagaatgcc gcccggttcg atggcattgg caagacctgc gagtgagttt tttacctgct
     4381 cattttcggg aaacagctca tacagtccag agacaatcgc cagcgtaggc cgcggagtta
     4441 acgcgctgag ttcctccggg ttaaacgcat cgccctgttc aaaacgcacc cgcccagaca
     4501 ttccccgttg ggcaatcatc tcttgcccct gtgcaacatt taactcactg taatcacgta
     4561 ataaaatatc gcttacggca ggctcgtttg ccagtgcatc cagtacatag cgcccatgcc
     4621 ctgcggcaat gtcaaccacg cggacggcta aacctttggc gtggagatcg gcaacggctt
     4681 gtttaatcag tatttgtaaa tgggttttgc gctggcgaat accgcgccag ccaacactgt
     4741 tcaggtagat tttgtcgatt aagcgcccga atgcgttact accttgcggt tgattttgat
     4801 agacataatc cagcgaactg cctgaatcaa agccggtgct cattccgaga tgcaggcccg
     4861 aagagtgcgt cccgaacaat ttcatcgcct ttcgcataaa gcgatacgct aaatcaaccg
     4921 gcgataatgg cacgggtcca ccagaaagta gccgccatcg atccgctgat ggtcctgtgc
     4981 agtcttcatg ctgataatca aatttttgcg atttgttagc atataaacga ctaataaagc
     5041 tttgcatttt ttcaaatgcc agcgccctgt tctcttcacc caacgtgtcg tgataaaagc
     5101 ctggcagcag atgcagctct ttcagagggc tacgtaatct ctgataaaaa tcaatctgcg
     5161 gttggcgatg caccacatag tcatcgcctg atatcagaag ttgcgtgggg agcgtaatcg
     5221 ccgccgcatc tctaataata cgttcagacg ttttgtagag atcgagcaag atattaacgg
     5281 caatcgcccg tgtgatcagc ggatcattat tgaaactcgc cccccgttgc cgatcgtggg
     5341 tcaaatagcg tcctttcaca taggaattaa taaaaaacag accacgcaga cgatgccata
     5401 acgccagcgc aggacgtgcc agcggcacat acaatttaac cttaaaggcc ggagaagcca
     5461 gcaccagccc gcgaattgca ggtgcataat catgaatcca tgtggcaacc agcactgcgc
     5521 cgacgctttg cgcgatcact accacctctt ccagtccaac ctggctgtcg ctggcagcaa
     5581 aacggacaaa ttcatcgaca tcccgcactg aacgcgcaag agatgggcta taaccacgcg
     5641 gccccgaact ttttccatgc cctcgggcat cccaggcata aaaagcagta tctggcatcg
     5701 ccagttcatc aacgagatgt tgtagacgac cagaatgttc atgcccgcga tg
//
LOCUS       DACAPB010000236         5694 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.237, whole genome shotgun sequence.
ACCESSION   DACAPB010000236 DACAPB010000000
VERSION     DACAPB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.237"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..490)
                     /locus_tag="IT904_004263"
     CDS             complement(<1..490)
                     /locus_tag="IT904_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAP3193955.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMI"
     gene            complement(513..1781)
                     /gene="lhgO"
                     /locus_tag="IT904_004264"
     CDS             complement(513..1781)
                     /gene="lhgO"
                     /locus_tag="IT904_004264"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAP3193956.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYHKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(1800..2777)
                     /gene="csiD"
                     /locus_tag="IT904_004265"
     CDS             complement(1800..2777)
                     /gene="csiD"
                     /locus_tag="IT904_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAP3193957.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            complement(3113..4117)
                     /locus_tag="IT904_004266"
     CDS             complement(3113..4117)
                     /locus_tag="IT904_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase"
                     /protein_id="HAP3193958.1"
                     /translation="MLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNE
                     RLHNTNLFNPKYHEKVVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKA
                     FSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLAMA
                     YMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGDEAWLNKGCSTYQQLYNEFHALY
                     IDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLIIINKGNEEVEGAWPNKLQP
                     GKYKNMGSNSVNIIINTRKIIPPGKAFMLRGGTLNINIPGRSALLLGKTGEPLNYLYL
                     "
     gene            complement(4127..5360)
                     /locus_tag="IT904_004267"
                     /pseudo
     CDS             complement(4127..5360)
                     /locus_tag="IT904_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311545.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1469 a         1272 c         1323 g         1630 t
ORIGIN      
        1 taatcatcgc cgccgggaag ttccacggcg tgatagctgc ggtgacgcca atcggctgct
       61 tgataacaat caggcgctta tcggcctgat gaccaggaat ggtgtcgcca taaatgcgtt
      121 tgccttcttc agcaaaccac tcaataaagg aggcggcgta gctaatttcg cctttcgctt
      181 cagccagcgg tttgccctgt tctagggtca tcaggcgcgc taaatcgtcc tgatgctcca
      241 tcaacaaatt gaaccagttg cgcagaatgt tggcgcgttc tttggcggtg agcgcacgcc
      301 aggcgggcag agcgcggttg gcggcgtcga tagcggcgcg ggtttcatca gcgcccattt
      361 tgggtacgct acccagcttg tcgccgttcg ccggattggt gacatcgatg acctcgccat
      421 tgttggcgtc cagccattcc ccgttaatca acgcctgctg gcggaataat ttactgtcgt
      481 taagtttcat tgttacgtcc tgttttcaaa ggttattgat taaatgcggc gtgtaaggca
      541 tccacactac gtgccgctcg cagcgtgcgt ccggggttac tctggcttgc caacagcgtt
      601 tgtaccttgc tgacaatatg cgcaccaata ggaattgctg atgtcgctgc cggggagggc
      661 gcattgcagg tgtggatcgt gcgcggggtg gtgacaaaca gaaaatcgtc aatcagcttg
      721 ccgtccggcg ataccgcctg cgcccgcaca ccggcgggcc agggctggag atcgcttaac
      781 gaaagccggg gacaatactt ttgcaccagc cgcagatagc cgcttttgca cagcgagttt
      841 ttcatctcgc ccagtcctga gcgtagatgg ttttgcagca cccggcgaat ccccgacgag
      901 cccaaaattt ccagcgtgtc gctaaacgag aagtcgcgct tgtggtagcc ttcgcgttta
      961 aaagccagca ccgcgtttgg cccgacggtc acgctgccat cgatcattcg ggtgagatgc
     1021 acgcccaaaa atggcatcgc ggggtcggga atggggtaaa tcaggtggtt aacaatctgg
     1081 ttatgctccg gcgcaaggcg gaaatactca ccacggaacg ggcagatgat aaagcccggt
     1141 tcgagtccga gcattttcac cagccggtca gccatcagcc cggaacagct aatcagcgtc
     1201 gatgcttcat attcgccgcc ctggcgggta cgtatcacca cgccgttttt atgctcactg
     1261 aggccgctga cttcggcgtt atagataatc tcgccgcctc tggcctggaa aatttttgcc
     1321 atcgccgccg ttacttcgcg gtagctgaca atgccgctgg acggcacaaa aataccgcca
     1381 agcccggtga tattcggttc gcgctcgcgc agttcatcgg cgtttaacca ctcgcgctcg
     1441 ataccgtttg ccgccgtgcg ttcccacaac gcgcgcatcc gttccatttc gagttcagag
     1501 gtggcgacca gcatcttgcc gcagttgtca tagcgaatgc cgttttgatc gcaaaaggct
     1561 ttagtggcgc ggtttcccgc caggcaaaac tgtgccttca ggctaccggg cgtgtaataa
     1621 accccggcat ggatcacgcc gctgttgtgg cccgtctggt gacaggccgg gccggactct
     1681 ttttccagca acgcaatgcg ggcgtccggg tagacatcaa tcagttgcat ggcggtcgac
     1741 atgccgatga tgccaccgcc aataatcaca aaatcataca tccgctcaat tcctttgcgc
     1801 tactgatgcg tctggtagtg gtgagtggcg taagcgaaat agccacgctg acgcatcagt
     1861 tcacggcgca gatccgggtg cggagtaaag cggtcgcgac cgtgcagcca gaacaggttg
     1921 ttaatcaaca ggaatttgcc aacgggaacg ggcacagaaa gaatgccttt gctggtttca
     1981 atggcgtccg aaagctcgct caaccacacg ccttcttcga agtcttttgg ctggacgaac
     2041 tggtcgatat aacgcatcac cgggcgaccc tgttgatcaa cgtcgaacac cggatggaaa
     2101 acatctttgc tgacgttttt actcggcggc gcggcaaagc gcattgggcg acgcgccagc
     2161 gggtggcgga aatagtggtc cagatgttcc cagtcatcga gatgcagcag caacgaattt
     2221 ccaccctgca tgttttgctc atcgattttc atcatcagca cgtaatcggt gatctcttcg
     2281 acatacgtgc cgtcgttgtg cagttccatc acgcggtgcg gctgacgcag atagctgtct
     2341 gagttatcga catttttcac cacgaaacgc gcgtaatact gaccgctcat cgcatcgaaa
     2401 tttgagcgac caatcagatg cgctaccgcc gttgccagct tcaccatctc atctgcctgc
     2461 gccacatcat cgatacccac cgcattgatc aacagcgcac cttcagcacg gtttagcagg
     2521 gtcttcaaca gcaatggttg cagctgattc gcacacagat cgtcaagaat tttgcctacc
     2581 cgaaaacgca gaaaggattt gtactccagc gcctgtacgg gccattcggc aacctgttcg
     2641 agaaactgtt tggtcgtctg ttcggtgaag gtaagttcca gcagacgcgg ggattgcgcc
     2701 gacgggatga aagtgaatcc gctatagtcc tggcctgaat cgacagcgtt attttgtacg
     2761 gcggtcagtg cattcatcag aagcgatcct cttatgagat gtagggtgac atggcgatgc
     2821 tcatttcgta gccataatct aaaaatatct acatttctga aaaatgcgca caaaagcgac
     2881 atattgtttt cttattgtga tcaaaagcaa caaattagta acatttaacg tagggtatta
     2941 aggcaaagtg agacaggaaa tgaataacta aagcaatagg ttagggggag ttaccatcag
     3001 agaacagatt atctggtcga taaaaaacag taaatggctt caatgcaaaa tgaccgttag
     3061 taaactaaac atattatctg ccccgtttta gcggggcaga tataacatta aattacaaat
     3121 agagatagtt cagcggttct cctgttttcc ctaaaagaag agcgctacgc ccaggaatat
     3181 tgatatttag agttccgcct ctaagcataa atgctttacc aggggggata atttttcggg
     3241 tattaataat aatgttaacg ctattactcc ccatgttttt atattttcca ggttgcaatt
     3301 tgttaggcca ggcaccttca acttcttcgt ttcctttatt tattataatt agtccatcat
     3361 gaggagagtg attaatgttc acagaatccc cacgactaaa ggccagaacg tttttagttg
     3421 caaatacacc ctcaaatgcg ctccatattc ctgctttatc tatatataat gcgtggaatt
     3481 cattgtagag ctgctgatag gtagagcaac ctttattaag ccacgcctca tcgccaacac
     3541 gcgtcgaccc atcataattt ttaatttcag tcgttgattt taatacttgg ccatcccagt
     3601 aaactaaagg cctgctgaac gggagagcgg ccatatatgc cattgctaat tgttcatctt
     3661 gttgatggtt aaatagaaag tcattgaaaa gattgccatt gttaggaaaa tcgtggttaa
     3721 tgttaatcaa tatacgtctt tttgatggga tgcttgagat tgtggaaccc ggttttgatg
     3781 caagggtttg gagatcgcca ccaaatgaaa aagcttttat cgtcttcatg agcaatgaaa
     3841 aatcaatggc gcttaaatca tctctttcat aaagcagcga tgacatttgt tcttcatcca
     3901 actgacaagt taccagatat tccatgtaat tcataacgac tttttcatga tattttgggt
     3961 taaataagtt agtattgtgt aatcgctcat tataattttt aagcggtggg gttattgatc
     4021 tttctatttg ttcatgtttt gagtgttttg ctgcatcgta acgtaaacct cgtacgccat
     4081 attttcgcat ttcacgaacg tattgatttt gcagcataat aacctggtca cttttggggg
     4141 ttgttttagg cattccattt aaacgatttc ttttaatgca tggaggtttt cccattcatc
     4201 cttagaaata ttatctttac ttgcttcgaa gtcttttgca gtgagatgta tatcttctgg
     4261 atggatttgt ttacgttctt ttgttactgg atctatagta aatggaatca aaacctgacc
     4321 ttgccaatag ccagggatac cactttctat atctttactg atcttcgctc gtatatcagc
     4381 agagggataa tccaggtcat ttttaccact acccccgatt cccataaagt taaaggtata
     4441 atcaggaatg atatcgatac caaatttttt agcatcttta accaatgcga taaaatcctg
     4501 caaatctccc attggattgt ggatggtaca aagatcttcg ggttgataag cgtgaaacca
     4561 gtaacctttt tcttcttcgg gagtttctgg tccgtcggca cggaaagttg tcactggagg
     4621 gatttgtata tacccataat ggcaacgtgc aaattcagac atattttctc tgatctgctg
     4681 gtaggtggca ttaaaagcat gaagaatcgc ttcgccaggt tttttggggg tgatgatttc
     4741 acattgtgat ttctgaacag cagtaagaga tttacgctca agtgtttgcc gtagatgttc
     4801 tagattgatt aatattttaa atgtttcttg ctgtccggaa ggtgacgtca cgttaatatt
     4861 atatacatcg gctgccagct catagtcagg aatgttaata gcatatgcaa catatttatc
     4921 ttttggggaa ggtttgtcag atataatgtg ggtgaaatgt tcagcacgac catactgata
     4981 aacaccgctg ttatcacctc tttcaatcat tgtatctcgt tgataaacct atgttttctt
     5041 tctcctatcc tgtctggcgc tgtaatgcta cgctgtgctg ctttattcaa ctctcggtaa
     5101 gttgtatctg gagaaaggga aattttataa tcgtttttta acagatcatt aataactttt
     5161 tctgcgtgaa aagttgatat aatttctctg tcttttaatc cttgagggta aactccacaa
     5221 gaattaataa tatttgttat acagtttaga tattctaatg tatttaattt ggaatttaga
     5281 tccccgtcag taatgttcca tgataacaat gtggggttgt cgataggtaa atttctgggt
     5341 cctggtttta tagaaaacat aataaagttc ctctaatggt agagtaatat gatgtatacg
     5401 gaaacataaa aatgtttaat tgttgtgaaa gtaattgcaa acagatgtcc tacttttatc
     5461 caatatttat atggaaaagg ataatgctta aatctgtttt attaattatc gatgtggtct
     5521 ggagaggcaa ctgtcaaaaa tcgctcatac gaaactaatg attagcagaa taagatctat
     5581 gaaagaatat attgctatac gaagtttatt tttatggagt gaaaagtaac agatatcata
     5641 atgatgcgaa aactcttcag ctaatgtaat aaagttatat tattagatac aaaa
//
LOCUS       DACAPB010000237         5601 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.238, whole genome shotgun sequence.
ACCESSION   DACAPB010000237 DACAPB010000000
VERSION     DACAPB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.238"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..200
                     /locus_tag="IT904_004268"
     CDS             <1..200
                     /locus_tag="IT904_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP3193959.1"
                     /translation="WGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICF
                     SRSVEIHEKVIGAFIEKHMFY"
     gene            complement(319..1140)
                     /gene="murI"
                     /locus_tag="IT904_004269"
     CDS             complement(319..1140)
                     /gene="murI"
                     /locus_tag="IT904_004269"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP3193960.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1162..2622)
                     /locus_tag="IT904_004270"
     CDS             complement(1162..2622)
                     /locus_tag="IT904_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP3193961.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(2615..2893)
                     /locus_tag="IT904_004271"
     CDS             complement(2615..2893)
                     /locus_tag="IT904_004271"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193962.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            complement(2874..3359)
                     /locus_tag="IT904_004272"
     CDS             complement(2874..3359)
                     /locus_tag="IT904_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP3193963.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKHEVQGKSTEPDLRSCANML
                     A"
     gene            complement(3671..3877)
                     /locus_tag="IT904_004273"
     CDS             complement(3671..3877)
                     /locus_tag="IT904_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193964.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYTEDAEDWLDSLPEQY"
     gene            complement(3914..4243)
                     /locus_tag="IT904_004274"
     CDS             complement(3914..4243)
                     /locus_tag="IT904_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193965.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            complement(4340..4567)
                     /locus_tag="IT904_004275"
                     /pseudo
     CDS             complement(4340..4567)
                     /locus_tag="IT904_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4599..5567
                     /locus_tag="IT904_004276"
     CDS             4599..5567
                     /locus_tag="IT904_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAP3193966.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWSTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRLIAETA
                     MYRMKQLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
BASE COUNT         1666 a         1233 c         1142 g         1560 t
ORIGIN      
        1 actggggcag ctatggccgg gaggtgccga agaataagca tctgaccggc aaaatattca
       61 cccaacgcat tgagcgtaat aatctgacgc tacgcacccg cattaagcgg ttgggtcgta
      121 aaacaatctg cttctcacgc tcagttgaga tccacgaaaa agtgatcgga gcgtttatcg
      181 aaaaacacat gttctactaa ttggatgcac tacccagtat gtaactgggt ttgtccataa
      241 aatgcacctt actcagttgg tgtccaactt ttggggtgca gttcagaagg acggggtttt
      301 acggtgcacc ggataaattt aaaactgcct gatactgaat gtatgaggtt tcgtaatgct
      361 caattcccct agtaccgttt tgtagatctc catacttcca ctagtattta tttgcaaagt
      421 tcctatattt gaggagttta ataaatttag ctgtcccaag tgatttttaa tagagatagc
      481 ctgatcatgt gcaggattaa taaattttat atccggtgca actgcttcaa aaaaattctg
      541 aacaattgga tagtgagtac agccgagaat aatttcattt actggatgga gggaaattaa
      601 atggttaaca tgttttttga tgtcattcaa gattgcagga gcattaagat taccttcctc
      661 aattaacatt gcaagatttt tgcttggttc tccaaataca tttattgttg aattcaattt
      721 ttttattagc tctttgtgac accccgtttt aatagtaaac tcggtagcga taataccaac
      781 atttttatat tgctggcgaa tcacatcttc tactgcaggt tcaatgatgg ataagatagg
      841 aaacggaaag ctcttagcat atccgctgta ttcttcggat tccaaagttg aggatatagt
      901 attacatgcg acagcaacta gtttgacatt ccttgactgt aaaaaagtca gcattttttt
      961 agtcaaactg atgatatcat cggcagaacg gttgccataa ggtgcattca tattatctcc
     1021 acaatagata atatcttcac caggaagcaa attaattatt tcctttaccg atgtcaatcc
     1081 acctacacct gagtctatta caccaattgg agattcagca ctaaacatat ttaacctcat
     1141 cttttgtagc ttatggaaaa tttaattact agttcttttt tcccaagaaa atggttcgaa
     1201 atcagagttt ggatctttcc agtcagattt tcggaaggcg taaattaata ttggaattaa
     1261 taaaaatagg gcaactaata ctaacaaata aagaacatag ctggttggac taccagttgg
     1321 gatttgctcg ggcggaataa aacttaatac aaaagccaga aaagacccta agaatccaag
     1381 tgttgcccag aaaaataaag caggggctcg atatgggcgt tttgtatcag gttgagaatg
     1441 tcgtagatag atagcactag cgaacatgag catatacata actaaataga gcataactgt
     1501 taactgtgat aaaatctgaa aagctgcttg aactgaaggc attatcacaa aagtaacaga
     1561 aagaactgag acaattatac cttgtacaaa cattagatgg tgccccattc catgacgatt
     1621 ggttttttgg aaaaacttag gtaaataacc agctttcgcg atcgctaata cacctgtatt
     1681 aggccctgca atccaagtaa ccaccccacc cagtacgcca attgctagca taaatgccat
     1741 cacagagcca agccatggca ctccagccca ttcgaacagc atatcgtagg caaccaacaa
     1801 actttgagtt agactaattt tatctgtagg aataataaag gcgaccccta aagtactgag
     1861 caggaatatt gcaatagtac ctatagaagc gatggtaatt gcaattgggt attttttagt
     1921 tggattatct aaatcgttaa catggaccgc attcatttcc atccctgcat aagcaagaaa
     1981 tatgctggcg gcaagaacaa tattctggaa gttagctata ttagggaaaa gatcacccca
     2041 acctaatggg atcaccgctg gtttaccgct gaaccacagg taggagaaac caagaataac
     2101 aaggataata gctggaataa ttgtccctat aataccgcac cacttagata cctgcgagaa
     2161 aataccaacg ccacgtaatg caacacaaat agctccccag taaacaaata gtacgaaaaa
     2221 taaaatatac catttattac tggcaattga ttctgccacc cccatatcaa catcaataaa
     2281 tgcaaccgag actgcaccaa atgtcagtac tgttggaaag taggggatga cttgtagcca
     2341 ggtcataagc attcctacaa aaccccagcg agccccaaat gcttctccta tccatcgaaa
     2401 tgccccccct ttttgtggat atgttgtagc tagctcagcc gcaacaaagg caacaggtac
     2461 taaaaacata attgcagcaa aggtatagta aaaaatggca cctaagccgt attctgcttc
     2521 agaaggtata aatcgtagac ttactacagc agttatattc atgatagcga gtgtccccac
     2581 tgtcattttc ttacttttgg gtgcgatatt agcattagac atttttatac tctcgaatat
     2641 ttttaaaata gaaacatata attacgatgt aaatataaaa actaatatta actttaacac
     2701 ttatcttaaa aacactaacg caatctgctt tacaaaaata agtaatacct aaagtcgtaa
     2761 cgacaccaat agtgttttca tcgacatgct tcaaaacttc tttagcattc attatcaggt
     2821 gatgatcatc aagtggaatt tcacacaatt tcacatcaca gtaacggcag aatttatgcc
     2881 agcatatttg cacaggatcg caaatcaggt tcggttgatt taccttgtac ttcgtgtttt
     2941 ttacaccact gccattttaa cgccaagcgg ccgagcatag cagcttcaga tgaaccaatg
     3001 gttgagcaac ctaaggtgtg tcgcgaatcg gggaaactcc acgaatcgga aagcatacgc
     3061 acacagcgac cttcaggttc cgccatttgt ggatattcat ctttataaat catgttttta
     3121 tcaatagcta aattaagcaa ctcacggatt tcatcatcaa gccatgtttg acagaaagta
     3181 gctaagtttt gtcgtgaatc cccatcaagg atgagctcat cacgtatggc gctgaataca
     3241 tttcgagatt tcttctcatc tacacgcaaa tgatatttat gcaatataaa tgaaagctca
     3301 agtgagctat gaatatcgac tgcttctcta taacaagaat tacctgattt atttttcata
     3361 ataaacgcca ctcaatacaa tcagattaaa cattacaaac acaatctgga ttttgcaatt
     3421 ttaaaactat caattttaga tatattttaa ttaatataaa accataaaca tatcaaaaaa
     3481 ttactttatt atggtcatgg tagatgtgtt tcaatgccaa cctggtctat tcctacatat
     3541 gtgagagcat atttacaaaa aaatcgttat tgtgatttca acggtgatgc tgaagtggca
     3601 cactaaattt ggccacctga acagaggtga tatgctcacc tcagaacaac atgacgtggt
     3661 gttattctgc tcagtactgt tctggcaatg aatccagcca gtcctctgca tcttccgtgt
     3721 aacactcgta gatgcagtaa ttggtgaaac tgatgtattt ggttagtaac tctgcttcgt
     3781 tgttcctgtg cgctcttctg gcctgcttac gtagttgttt cgcttctgcc cgctggcctt
     3841 cgagtatctc cagcctgtct gcaatatctt gcttcatgtt cagctcctcg cgcgcatagt
     3901 acttgtccga aaattaatcg cccgtcccat cattaatatc gattcctacg ctgtgtaact
     3961 ggctatgaat gtctctgcga atgagaatta aacgcctgat gaactccatt tcctccgcgc
     4021 tctggttgct cggccccgat ttcatgtcga tgattttatt gatagttcca agcgaatatt
     4081 cagcccacag acgaagcgtt tggcgcttca tggcctgttt gctcagtggc gaatgtttca
     4141 ggtctaagac atgttctgcg agggtcttag cgatgccctg catgatgcgt agtgccaaca
     4201 tgatgatttt ctccggactt gtgctaatat agttcccgaa cattgtttcg agactgctta
     4261 tcctgtttgt cttgtccgat ttgttcattg gtcacattgt taagtcagcc ggggtaattc
     4321 ccggctttct tttatccgat cagtaccgaa tccctatcgc attgaatacg cgtttccgtt
     4381 gctcagcttc aggatcggtg gccatttctg gttctggctc cggcatagac ggggcggcat
     4441 caaaatgctc aatttcacga ccgaaatacg agttcaggtc aatacctgcc gaactatcca
     4501 ctccatcaaa atagcgacgc tgttcctcgg tgagacagct acccagacgt tcctcaacct
     4561 gcgccaggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
     4621 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca
     4681 cctacaataa agccctcatc aaccgtggct ccataacttt ctggctggat gatgaagcta
     4741 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctattctg
     4801 accttgccat cacgactgtg ctggtcatta aacgcgtatt caggctgacc ctgcgcgctg
     4861 cgcagggctt tattgattcc attttttctc tgatgaacgt tccgctacgc tgtccggatt
     4921 acagctgtgt cagcaggcgg gcaaagtcgg ttaacgtcag tttcaaaacg cccacccggg
     4981 gtgaaatcgc acacctggta attgattcca ccgggctgaa ggtcttcggt gaaggcgagt
     5041 ggaaagtcaa aaagcatggc caggaacgcc gccgtatctg gcgaaagttg catcttgctg
     5101 ttgacagcaa cacacatgaa gttgtctgtg cagacctgtc gctgaataac gtcacggact
     5161 cagaagcctt cccgggcctt atccggcaga ctcacagaaa aatcagggca gccgcggcag
     5221 acggggctta cgatacccgg ctctgtcacg atgaactgcg ccgcaaaaaa atcagcgcgc
     5281 ttattcctcc ccgaaaagga gcaggttact ggcccggtga gtacgcagac cgcaaccgtg
     5341 ccgttgctaa tcagcggctg agcggaagca atgcacggtg gaaatggaca acggaatata
     5401 accgtcgctt gatagcggaa acggcaatgt acagaatgaa gcagttgttg ggagattcac
     5461 tgacgctgcg tgactacgat ggtcaggtag cggaagctat ggccatggtg cgtgcgttga
     5521 acaggatgac aaaggctggg atgccagaaa gcgtgcgtat tgcctgaaaa tccagccagc
     5581 tacagggtcg ttcgcaagaa a
//
LOCUS       DACAPB010000238         5579 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.239, whole genome shotgun sequence.
ACCESSION   DACAPB010000238 DACAPB010000000
VERSION     DACAPB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.239"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..239
                     /locus_tag="IT904_004277"
     CDS             <1..239
                     /locus_tag="IT904_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HAP3193967.1"
                     /translation="EVGYSEFLQWALSGDLDTFYENVRWQQWREDVIKLSATEAFTFY
                     PFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(280..774)
                     /locus_tag="IT904_004278"
     CDS             complement(280..774)
                     /locus_tag="IT904_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193968.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(989..2629)
                     /gene="pgm"
                     /locus_tag="IT904_004279"
     CDS             complement(989..2629)
                     /gene="pgm"
                     /locus_tag="IT904_004279"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAP3193969.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(2655..3200)
                     /gene="seqA"
                     /locus_tag="IT904_004280"
     CDS             complement(2655..3200)
                     /gene="seqA"
                     /locus_tag="IT904_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAP3193970.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3385..4149
                     /gene="ybfF"
                     /locus_tag="IT904_004281"
     CDS             3385..4149
                     /gene="ybfF"
                     /locus_tag="IT904_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP3193971.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4220..4582
                     /gene="ybfE"
                     /locus_tag="IT904_004282"
     CDS             4220..4582
                     /gene="ybfE"
                     /locus_tag="IT904_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAP3193972.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            4722..5252
                     /gene="fldA"
                     /locus_tag="IT904_004283"
     CDS             4722..5252
                     /gene="fldA"
                     /locus_tag="IT904_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAP3193973.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5462..5548
                     /gene="uof"
                     /locus_tag="IT904_004284"
     CDS             5462..5548
                     /gene="uof"
                     /locus_tag="IT904_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAP3193974.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
BASE COUNT         1359 a         1411 c         1377 g         1432 t
ORIGIN      
        1 tggaagttgg atattctgag tttcttcagt gggcattatc cggtgattta gataccttct
       61 atgaaaatgt acgatggcag caatggcgag aagacgtaat aaaattatcg gcaacggaag
      121 ccttcacgtt ctaccctttc ttatgggtac aaagtgaaga agcaagaaca agaaaagtta
      181 tttcgcttac tgaactctgg gaaatgcagt atcaaatgaa agaaacattc acgcaataag
      241 acatgttcag gtccgggttt catttccgga ctcactattt taatgtgtat ttatcgcttt
      301 caattcttgc tggcaatttt ctgactggca gcgtcgtaac tcaataatcc cgacatgcat
      361 catcgagcca aaaaatccgc tttcgctgac ataatcctca ttgactatcg gtcgagtgcc
      421 gggcttgccc tggcaaaccc gcataaaaaa tgaggtgtag cattgccaac cgggatttaa
      481 accgaccttt tcttgtgctg acatattatg aagataagta agttcttttt cgttcccgcc
      541 tgacaactgc cagcggcggg tattatcagg taagttatca ccacggcaat ttgtcttaca
      601 ccgggaaggt gcaatattta ccgcaatgct acccgttaat cctctggttt tatctttagg
      661 ggtcagtaac cagtcgccag ctaatgatga ttgttcaggt tgggcgcatg cagtcagcag
      721 taatgtggcg gtaattagtg acactatgac gaccagactc ctgttggtct tcattgggat
      781 ttccttgagc tactcagttt gttgacaaag tgttcgttgt ttttgccgga tgcggcgtga
      841 acgccttatc cggcctacaa aatcgcgtaa attcaatata ttgcaccatc ctcgtaggcc
      901 tgataagcgt agcgcatcag gcaattctgt gtttgtcata cgtaaaaaag ggcgatcttc
      961 cgaccgccct ttttcttatt taatgtgttt acgcgttttt cagaacttcg ctaacaatct
     1021 caaccgcttc tttctcgatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga
     1081 tcttatatgc gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca
     1141 ctttcagacc gccaatagaa gccccgttgc ccggagcagc agtcaggcgc gcggtgatcg
     1201 ggtcacctgc cagggtgctg gcgctcacca tttccggaga cagcttagac agcgccgctt
     1261 tttgtgcgga agtcgcagct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg
     1321 ccagttcgtt gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca
     1381 gcagacacat gatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga
     1441 aggaagcccc tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa
     1501 caaaccattt gaaacctacc gggacttcta ccagcttacg acccaagtca ttgaccacac
     1561 ggtcgatcat cgcagaagaa accagcgttt taccaacggc aacatctttg ccccactgcg
     1621 gacgatgctg gaacaggtaa ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac
     1681 ctgccggagt gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga
     1741 acttatcacg cagtgccagc aggcccgcca tcgcacactc ggaggagcag tccatacgga
     1801 tcgcgccgtc tttatcaagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga
     1861 tagtcaggtt gaggttgtaa tactcgccaa tacgcttcca gtattcgata ccggaaccgc
     1921 ccagcggatc aacgccaagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat
     1981 cggccagccc ttccacgaac ggctgcacca gatcctgctc tttcacatga ccggatgcca
     2041 tcgcttcgtc gagggagata cgcttcacgc ctttcaggcc atcggccagc agtgcgttgg
     2101 ccctgtcttc caccactttg gtgacgttgg tatcagccgg gccaccattt ggcggattgt
     2161 acttgatacc accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca
     2221 gcgggccacc ttttttattg tgaaccagga tggcattgga aacggcaggc gttggagtga
     2281 agccattgtt ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg
     2341 aaatgaacgc aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga
     2401 tgccgttttt cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt
     2461 taaagctgtg gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct
     2521 ccgcattccc tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa
     2581 tcaaatcact ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct
     2641 ttgtctgcaa cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc
     2701 atcgactgca tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc
     2761 acccaatacg gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc
     2821 gtttgttcat ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg
     2881 gcaaacgcct gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc
     2941 gctcgctttt gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc gcgaaccttg
     3001 tctttaatcg ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct
     3061 ttcgtcaccg gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg
     3121 tcggatgcgc tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca
     3181 tcaacttcaa tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca
     3241 ggagtatatg aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg
     3301 gaataaacct tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga
     3361 caacgcgaaa aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac
     3421 cagcacaata attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt
     3481 ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac
     3541 cacggtcttt caccgagaga gccggtaatg aattacccgg cgatggcgca ggatcttgtt
     3601 gataccctag atgcactgca gatcgacaaa gcaacattta tcggtcactc catgggcggt
     3661 aaagcggtaa tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc
     3721 gatatcgcac cggtcgacta tcacgtacgc cgtcatgatg agattttcgc agctatcaac
     3781 gcggtcagtg aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat
     3841 cttaatgaag aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc
     3901 tttaacgtgc cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg
     3961 gcatgggatc accctgccct gtttatccct ggtggtaatt ctccgtatgt tagcgagcag
     4021 taccgtgatg atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg
     4081 ggtcactggg tccatgctga aaaaccagat gcggtattac gcgctatccg tcgctatctc
     4141 aatgattaac gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt
     4201 ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct
     4261 gattgtttcc cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt
     4321 agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga
     4381 tgaacagctg cgtattaata aacgcaacca actaaaacgc gacaaagtac gtggccttaa
     4441 gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc
     4501 gcgcaatatg agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct
     4561 gcgtagtcag ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc
     4621 gtttgctgat tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt
     4681 tccaccccca tttcaataag tttcaagagg ttatttcact catggctatc actggcatct
     4741 ttttcggcag cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg
     4801 gtaaagacgt tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt
     4861 atgacattct gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg
     4921 atgacttctt cccgactctc gaagagattg atttcaatgg caaactggtt gcgctgtttg
     4981 gttgtggtga ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg
     5041 acatcattga accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt
     5101 tcgaagcatc aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag
     5161 accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag
     5221 agttgcatct cgacgaaatt ctcaatgcct gatgtgatgc ggcgtagact catgtctacg
     5281 ccgtattaat agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg
     5341 tacctgtaca atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctatgccac
     5401 gtttttatta acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat
     5461 aatgatacgc attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt
     5521 agtaacagga cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctg
//
LOCUS       DACAPB010000239         5554 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.240, whole genome shotgun sequence.
ACCESSION   DACAPB010000239 DACAPB010000000
VERSION     DACAPB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.240"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..2150
                     /locus_tag="IT904_004285"
     CDS             <1..2150
                     /locus_tag="IT904_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP3193975.1"
                     /translation="VVAARGTERQAAAEVKLTAAQASLTRNIAARTAAQTTLNTVTSV
                     GSRLLSGALGLVGGVPGLVMLGATAWYTMYQNQEQARESARQYAATIDEIRQKTSAMS
                     LPEASDNEEKTRQALDEQNRLIDEQKSKIKSLQEKIAGYQYVLANPGWTTDNGFMINH
                     MTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERRVALIRQQAAEQN
                     KAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQTALNNSER
                     ELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYINNSLDAWRNNQANKPKAHK
                     KTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQGELSTLSEAQKKTLLQNA
                     ALIDQKKIREQLAAYESSLADSNASTRASNDAQLLGYGEGSRMRERLQEMWSIRHEFE
                     QKNNELLRQYQAGEIEEALWKQEKELNKKYLEERLSDQQDYYAKADALRNNWNAGLQE
                     GLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWGSSILQEVSKILM
                     NAAIVNGLKSLSGAGGWLGTVGGWISGAVANAKGGVYTSANLSAYSNTIVDTPTYFAF
                     AKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGGGFVYSPVYHISIQNQGSNGE
                     IDARSARGLVDLIDSRVVSIMQSSRRDGGLYSA"
     gene            2128..2484
                     /locus_tag="IT904_004286"
     CDS             2128..2484
                     /locus_tag="IT904_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193976.1"
                     /translation="MEDCTVPEPEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGG
                     LNADLKTFKPVFRVTDEYSRAALDSFLSRHAGIRAFLWRPPKHNRTVRVVCREWSISD
                     NAMYTDFNCTFEEVTH"
     gene            2484..3182
                     /locus_tag="IT904_004287"
     CDS             2484..3182
                     /locus_tag="IT904_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP3193977.1"
                     /translation="MQDIQQETLNECTKTEQSALVVLWEIDLTEVGGERYFFCNEQNE
                     KGESVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGIAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            3188..3931
                     /locus_tag="IT904_004288"
     CDS             3188..3931
                     /locus_tag="IT904_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3193978.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMAPEDWLRAEMQGEIVALVHSHPGALPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNMEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHVSAFTGICNDLAAASTFV"
     gene            3829..4476
                     /locus_tag="IT904_004289"
     CDS             3829..4476
                     /locus_tag="IT904_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP3193979.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     AMQIPAFRQKLNEGWYQVRIAGRDAGENELSARLNEPLKNGAVIHIVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPAGWLGAAALSGMYAAGASMILGGVAQMLAPKASTATTTSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            4537..>5554
                     /locus_tag="IT904_004290"
     CDS             4537..>5554
                     /locus_tag="IT904_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP3193980.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVL"
BASE COUNT         1362 a         1306 c         1725 g         1161 t
ORIGIN      
        1 cggttgttgc tgctcgcggt accgaaaggc aggccgcagc agaagtgaag ctgacagctg
       61 ctcaggcgtc acttacccgt aatattgcgg ccagaacagc ggcacagaca acgctgaata
      121 ctgtcacgtc agtggggagt cgtctgttaa gtggtgcgct ggggttggtt ggtggtgtgc
      181 cgggactcgt catgctgggg gcgacggcct ggtacacgat gtatcagaat caggagcagg
      241 ccagagaatc tgcacgccag tatgccgcaa caatcgacga aattcgccag aaaacgtcgg
      301 caatgtcgct tcctgaagcg tcagataatg aggaaaagac gcggcaggca cttgatgagc
      361 aaaacaggtt aattgacgag cagaaaagta agattaaatc cttacaggaa aaaattgctg
      421 gctatcagta tgtgctggca aacccgggct ggacaaccga taacggtttt atgattaacc
      481 acatgacgtc ggtaaaaact gtcacagaag ggcttgcaga agcaacaaat caactggcag
      541 ttgaacagtc ccgtctcaca caaatgcagg gcaaagcgca atccattcag gatgtgcttg
      601 ccgggctgga ggagcgacgg gtggcgttga tccgtcaaca ggccgcggaa caaaacaaag
      661 cgtatcagtc cctgttgatc atgaatgggc agcataccga gtttaatcgc cttctcgggc
      721 tcggtaatga attacttcag cagcgacagg ggctggtgaa tgtaccgtta cggctaccac
      781 aggcaaccct ggatgataaa cagcagactg cactgaataa cagcgagcgc gaactggctc
      841 tgtcccgcct gaagggggaa gcccgtgagc gtgcccgctt gggttatgct gcggatgatc
      901 tcggctttgt gggagaggcg tatcagacag ccagacagaa ttatatcaat aactcactgg
      961 atgcctggcg aaataaccag gcaaataaac ccaaagcgca taaaaagacc gaagcggaaa
     1021 aaacagaaga tatttataaa cggctgatta aacagcaaaa agaacaaata gcactggcag
     1081 ggcagaatac tgaactggct aagatgaaat atcaggtcag tcagggcgaa ttatcaaccc
     1141 tgtcagaagc gcagaaaaaa acgcttttgc agaatgcagc actcatcgac cagaaaaaga
     1201 ttcgtgagca gcttgctgcg tatgaaagca gcctggcgga cagtaatgcc agtacccggg
     1261 cgtctaacga cgcgcagtta ctgggatatg gtgaaggctc acggatgcgt gaacgactcc
     1321 aggaaatgtg gagtatccgg catgagtttg agcagaaaaa taacgagctg ctgagacagt
     1381 atcaggccgg agaaattgaa gaagccctgt ggaaacagga gaaagaactg aataaaaaat
     1441 atctggaaga gcgtctcagc gatcagcagg attattatgc aaaggccgat gctttacgta
     1501 ataactggaa tgcaggactc caggaggggc tgaccaactg ggcagacagt gccaccgatt
     1561 atgcttcgca ggcggcagat gctgtcgttt ccactatgga cggactggta tcaaatattt
     1621 ccgatgcact ggccggaaat gttgtggact ggagaaactg ggggagttca attctccagg
     1681 aagtttcaaa aattctgatg aatgcggcca ttgttaacgg actgaagtca ctctccggtg
     1741 ccggagggtg gcttggtacg gtcggcggat ggatttcggg ggcggtggca aacgcaaaag
     1801 gtggtgttta cacatcagca aatctgagtg cttacagtaa cactattgtg gatacaccga
     1861 cgtattttgc ttttgcgaaa ggtgccgggt tgatgggcga ggccgggcct gaagcaatca
     1921 tgccactgac acgggcagcg gacggctctc ttggggtcag ggccattgga aatgtgaatg
     1981 gtggcggtgg atttgtttat tctcccgttt atcacatcag cattcagaat caagggagca
     2041 atggcgagat agatgcgcgc tcagccaggg gactggtgga tctgatcgac agcagggttg
     2101 tgtcaattat gcagtcatcg cgtcgggatg gaggattgta cagtgcctga gcctgaagtt
     2161 tttaactgga tcccccgtga ggggatggag acgacacgaa agccatcagt tattacggta
     2221 aagtttggtg acggatatga acagcgacgg gctggtggcc tgaatgcgga tctgaaaacg
     2281 tttaaaccgg tatttcgtgt cacagatgaa tattcccgtg ccgcgctgga cagtttttta
     2341 tcccgtcatg ccgggattcg tgcttttttg tggcgtccgc caaaacacaa caggactgtc
     2401 cgggttgtct gcagggagtg gagcatttcg gataatgcca tgtataccga ttttaactgt
     2461 acctttgaag aggtcactca ctgatgcagg atatacagca ggaaacactc aatgaatgca
     2521 ctaaaacgga gcaatccgcg ctggtcgtgc tctgggaaat tgatctgaca gaggtcggcg
     2581 gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagtcggtc acctggcagg
     2641 ggcgacagta tcaggcgtac cctattcagg gaagcggctt tgagatgaac ggcaaaggag
     2701 ccagtgcaag accaacgctg aaagtctcta acctgcacgg catggtcacc ggcatagcgg
     2761 aagacctgca gagtctggtc ggcggaacgg tggtcaggcg taaggtttac gcccgttttc
     2821 tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag gaggtaatca
     2881 gccgctggcg cattgagcag tgcagcgaac tgagcgcggt cagtgcctcc tttgtactgt
     2941 ccacgccgac ggaaacggac ggcgctgttt ttccggggcg catcatgctg gctaatacct
     3001 gcacctggac ctatcgcggt gatgagtgcg gttatcacgg tccggcggtc gcggatgaat
     3061 atgatcagcc gacgtccgat atcacgaagg ataaatgcag caaatgcctg agtggctgta
     3121 agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa ctttcgcagt
     3181 aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat gtgcgccagc
     3241 ggagtcgtgc ggcttcgtgg tgagaacgcc ggaaggggaa agatattttc cctgcgtgaa
     3301 tatctccggt gagccggagg cgtatttccg gatggctccg gaggactggc tgcgggcaga
     3361 aatgcagggt gagattgtgg cgctggtcca cagtcacccc ggtgctctgc cctggctgag
     3421 tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg tctgccgggg
     3481 ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg agcacggggt
     3541 gacggactgt tacacgctgt tccgggatgc ttaccatctg gcgggaattg agatgcctga
     3601 ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg ataatatgga
     3661 ggcaacgggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg tgctgctgtg
     3721 ctgttttggt tcatcggtgc cgaatcatgc cgctatttac tgcggcgacg gcgagctgct
     3781 gcaccatatt cctgaacagc tgagcaaacg agagaggtat accgacaaat ggcagcgacg
     3841 cacacactcc ctctggcgtc accgggcatg gcacgtatct gcctttacgg ggatttgcaa
     3901 cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg ccgaagccat ccgggcgctg
     3961 gccatgcaga tcccggcgtt tcgtcagaaa ctgaatgagg gctggtatca ggtgcgcatt
     4021 gccgggcgtg atgcaggcga aaacgaatta tcagcccgtc ttaatgagcc gctgaaaaat
     4081 ggtgccgtga tccacatcgt tccgcgtctt gcgggagcaa aaagtggcgg tgtgtttcag
     4141 gcggtgctgg gtgcggcgct gattgcagtg gcgtggtgga accctgcagg ctggctggga
     4201 gctgcggctt tatcgggcat gtatgctgcg ggggccagta tgatccttgg cggtgtggcg
     4261 cagatgctgg caccgaaagc cagtaccgca acaacgacca gcacggataa cggtaagcag
     4321 aacacgtatt tctcgtcact ggataacatg gttgcccagg gcaatgttct gcctgttctg
     4381 tacggtgaaa tgcgcgtggg gtcgcgggtg gtttctcagg agatcagcac ggcagacgaa
     4441 ggggacggtg gtcaggttgt ggtgattggt cgctgaggca aaatgtttta tgtgaaaccg
     4501 cctccgggcg gttttgtcgt ttatggagcg tgaggaatgg gtaaaggcag cagtaagggg
     4561 cataccccgc gcgaagcgaa ggacaacctg aagtccacgc agttgctgag tgtgatcgat
     4621 gccatcagcg aagggccggt tgaaggtccg gtggatggat taaaaagcgt gctgctgaac
     4681 agtacgccgg tgctggacac tgaggggaat accaacatct ccggtgtcac ggtggtgttc
     4741 cgtgccggtg agcaggagca gacaccgccg gaggggtttg aatcctccgg ctccgagacg
     4801 gtgctgggta cggaagtgaa atatgacacg ccgatcaccc gcaccattac gtctgcaaac
     4861 atcgaccgtc tgcgctttac cttcggtgtg caggcactgg tggaaaccac ctcaaaggga
     4921 gacaggaatc cgtcggaagt ccgcctgctg gttcagatac agcgtaacgg tggctgggtg
     4981 acggaaaaag acatcaccat taagggcaaa accacctcgc agtatctggc ctcggtggtg
     5041 gtggataacc tgcctccgcg cccgttcaat atccggatgc gcaggatgac accggacagc
     5101 accacagacc agctgcagaa caaaacgctc tggtcgtcat acaccgaaat catcgatgtg
     5161 aaacagtgct acccgaacac ggcactggtc ggcgtacagg tggattcgga gcagttcggc
     5221 agccagcagg tgagccgtaa ttatcatctg cgcgggcgta ttctgcaggt gccgtcgaat
     5281 tataacccgc agacgcggca atacagcggt atctgggacg gaacgtttaa accggcatac
     5341 agcaacaaca tggcctggtg tctgtgggat atgctgaccc acccgcgcta cggcatgggg
     5401 aaacgtcttg gtgcggcaga tgtggataaa tgggcgctgt atgtcatcgg ccagaattgc
     5461 gaccagtcgg tgccggacgg ctttggcggc acggagccgc gcatcacctg taatgcgtac
     5521 ctgaccacac agcgcaaggc ctgggatgtg ctca
//
LOCUS       DACAPB010000240         5544 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.241, whole genome shotgun sequence.
ACCESSION   DACAPB010000240 DACAPB010000000
VERSION     DACAPB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.241"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            148..453
                     /locus_tag="IT904_004291"
     CDS             148..453
                     /locus_tag="IT904_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAP3193981.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            579..2183
                     /locus_tag="IT904_004292"
     CDS             579..2183
                     /locus_tag="IT904_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAP3193982.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQGLPRETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(2195..3007)
                     /locus_tag="IT904_004293"
     CDS             complement(2195..3007)
                     /locus_tag="IT904_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193983.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLAQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(3011..3796)
                     /locus_tag="IT904_004294"
     CDS             complement(3011..3796)
                     /locus_tag="IT904_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAP3193984.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPLPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(3793..4461)
                     /locus_tag="IT904_004295"
     CDS             complement(3793..4461)
                     /locus_tag="IT904_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP3193985.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(4525..5163)
                     /locus_tag="IT904_004296"
     CDS             complement(4525..5163)
                     /locus_tag="IT904_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAP3193986.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(5176..>5544)
                     /locus_tag="IT904_004297"
     CDS             complement(5176..>5544)
                     /locus_tag="IT904_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAP3193987.1"
                     /translation="ALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPV"
BASE COUNT         1440 a         1438 c         1288 g         1378 t
ORIGIN      
        1 atggtggggg tcggtggttc gagcccactc ggacgcacca gattttctta atctggtctt
       61 ttcctttttc cctctgtttc ttctctgtat ccaatacgtt aaaagattta cactatcttc
      121 gtatgcgtta tcagaaggag aatcgctatg gcaactttgt tacaacttca ttttgctttt
      181 aatggcccat ttggcgacgc aatggctgag cagcttaaac cgcttgctga gtcgattaat
      241 caggaacctg gttttctgtg gaaggtatgg acagaaagtg aaaagaacca cgaagccggt
      301 gggatctatc tttttactga tgaaaaaagc gcccttgcct atctggaaaa acatactgcc
      361 cgacttaaaa atctcggcgt tgaggaagtt gtcgccaaag ttttcgatgt caatgagcca
      421 ctgagtcaaa tcaatcaggc aaaactcgcc tgacagaatt taatcaaggg cggttagcgc
      481 ccttttcatc cctgtctgaa atttctcaaa ttctaaaaat ctcaaccaaa cttatctgat
      541 aacactaaat tcgaaagaat gcgtacaggt aagtaacaat gaaaaaaatt gctattgtgg
      601 gtgccgggcc tacggggatc tacaccttat tctcgcttct acagcaacaa actccacttt
      661 ctatttctat cttcgagcag gctgacgagg ccggtgtcgg gatgccatac agtgatgagg
      721 aaaactcaaa aatgatgctg gcaaatattg ccagtattga aataccgccg attaattgta
      781 cgtatctcga atggctacaa aagcaagaag ccagccatct ccagcgttat ggcgttaaaa
      841 aagaaacctt gcacgatcgt cagtttttac cgcgaattct gctgggcgaa tatttccgcg
      901 atcaattttt acgattagta gaccaggcac gaaagcaaaa atttgcagtg gctgtttatg
      961 aatcatgcca ggttaccgat ctgcaaatta caaatgctgg cgtcatgctc gctacaaatc
     1021 agggtttacc cagagagacg tttgatttag cggtgattgc cacgggtcac gtctggcctg
     1081 atgaagaaga agcaacccga acgtattttc ccagcccgtg gtcaggcctg atggaagcaa
     1141 aggtcgatgc gtgtaacgtg ggtattatgg gaacatcctt gagcggactg gatgcggcaa
     1201 tggcagtggc tattcagcat ggttcgttca ttgaagatga taaacaacac gtcgtttttc
     1261 accgcgataa cgcaagtgaa aagctaaata tcacgttgat gtcgcgcacg ggtattttac
     1321 ccgaagccga tttctattgc cctattccct acgagccctt acacattgtc accgatcagg
     1381 cattaaatgc tgagattcaa aaaggcgaag agggcctttt ggatcgggta tttagattga
     1441 tagtagagga aatcaagttt gctgatccag actggagcca acgcatagcc ttagagagcc
     1501 tgaatgtcga ttcctttgct caagcctggt ttgccgagcg caaacaacgc gacccatttg
     1561 actgggcaga aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa catactgttc
     1621 cctggcgtta tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt ccacatctga
     1681 atgaacacga ccataaacgg ttcagtaaag gccttgcccg ggtttttatc gataattatg
     1741 cggcaatccc ttcagagtct attcgtcggc tgctggcctt acgtgaagcg gggatcattc
     1801 atattctcgc cctcggtgaa gactacgaaa tggaaattaa tgagtcgcgc accgtcctga
     1861 aaacggaaga caacagctac tcgtttgacg tttttattga tgcccgcgga cagcgtccgc
     1921 ttaaagtgaa agatattcct ttccctgggc tacgcgagca attacagaaa acaggggatg
     1981 aaatccctga tgttggcgaa gattatacgt tacagcaacc cgaagatatt cgtggacgcg
     2041 tagcgttcgg cgcgttgccc tggttgatgc acgaccagcc tttcgttcag ggacttacgg
     2101 catgtgcaga aattggtgag gcgatggctc gggcggtcgt aaaacctgca tcccgtgcac
     2161 gtcggcgtct ttcgtttgat taaaggtaaa gctatcagca ctcaacaaac tgatgctgtt
     2221 cattaagacg atagcggact ccggctcgaa tattgttttc accttcaatg gcgacggcga
     2281 agcgaactct ttcgccgtca tgatatgcca gtgcggcgct tccccctggc ccgagaataa
     2341 ttgagtcgac cacgcccgtg ctcactaccg ttgaattttc accgctggca atgatgtgta
     2401 cattgtcgcc gctgttggca attctggcgt ttgcgccaaa gctggcgatt tgcgccaggt
     2461 caccattact ggcgacatga caccgttcac ccaatgtaca aacgcgtact cgcattccgg
     2521 tgttcgcaat ccgactacta tctccagcgc tgcttatccg cgaagaatta ccggttacag
     2581 caacccgtgc gcgttcaccg gagctgccaa tgtgactgtt aaagccgaca cttccaatcc
     2641 gcacgctata acccatactg gcaatttgcg cggcataccc agcggttgcg attcgggcgt
     2701 tatcctcaca acagccgatg cgagcatgat cgtcgctgcg ggcaaactga ctgcaaaatg
     2761 gtatctctcc ggggtctgtg gccagttttt gcatagagga aacttcctga tgcgcgaagt
     2821 tttcatccgc cagccatttt ttccacgcat actcaaccag actctccgcc cagtccgtat
     2881 atccttcctc aatcagtgca tgatgaatat cggcataact accgccgcgt gggaaatggc
     2941 gcaggaacca gcgatacatc accgctccga tacgccattc tcgcaggtct gctcgtgtaa
     3001 tgatcattcg ttagtgccgg tgatagccat cgaccatgct tttaaaggtt tcgtgtggcg
     3061 tacgccccgt ggtgcaaaga taaagatgac cgaagataaa aaagaggctt ataaatgcca
     3121 gagcaaaatg cgcctgcaat aaccagtatc ttacgccagg aaacacatct cccacggctt
     3181 gcggatagag acacaacagc cccgtcaata gtaacaacgg cagcaatcca tacatgacac
     3241 caacataggc gacctgctgt aagggattaa atttagactg tgttgttgcc ggtaaaggat
     3301 gttcttcccc ctgcataatg ccaaacaaat aaaatcgcgt ttgttttgcc gctcgttcca
     3361 gccacccctg acgacgaatg cgatagtggt gaccattatc cccaacggca ttgatcagca
     3421 caaagccgag ccagcatgcc agtaacaaaa atccgcaaac ttcatgcacc gcaaccagac
     3481 ttttaaccgc agttgcgccc accatcgcaa agtgatttat cagcccactt gccagcaaca
     3541 atacaaagag taacgcattc gaccagtgcc ataatctgac cgctttgctg taaagataaa
     3601 ccttctcacc atgatcggtg gctgatttct tcacgccacg agcacgaagc aaggcgtgca
     3661 gccccagcac caaccacatc ccaaccagca atactccggc aatgatcaac cacaccggcc
     3721 aaaattcggg agtgaactgt ggcacatagt tcgctaactg gctctgaaac tgttcagcat
     3781 gttgcgacgg gttcatacat tttccttttt aatcaaatgt tgaccgaacc ggcgatacag
     3841 atgcggtttc ccggcaccag gtagctgata ttgatagtat ttattatcct gcaaccacgc
     3901 ctgaatttct gggctatctt cgcgaccaaa gatcaatgcg tgttccggac aggcgctgac
     3961 gcaaatgggc ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac atttatctgc
     4021 cactttagtg acagggttaa ggtagcgaac ctggtacgga caagcgccaa tgcaataact
     4081 gcagccgatg cactgcgatt tttctacccg cacgatcccc tgctcatcac gccacgatgc
     4141 ccccgtcggg catacgtcga tgcacggagc atcttcgcag tgctgacatg actggcgaaa
     4201 aaagtgatac tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta tcgataaacg
     4261 gcttccctga gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa tattacagcc
     4321 attgcaccgt gactcgtcat gaatcatggc ataccgaact tccttttctt gcctcgtgtt
     4381 cgctaacaga gaagacgcag aaccagtaaa aaagattact gttcccatcc ccagaacaaa
     4441 tttgcgtcga gtgaaggaca tctgattatc ccctaatgca gtggctgcaa ctgtccaccc
     4501 tatccatgac atctccctga aatttcaggg gttctgaaac tgctctacct cgaaccattc
     4561 gcataagcca taagccttgc cataggtcat aaatcgctcg ctaaacaagt acaacaccgc
     4621 cgtcgatgac tccacctgcc gtatatcggc atattcaggt gcaacatcca tcgcggcgat
     4681 cgcggcttca atctgcgctt cctggaagta atatggcgct tgcatcagca tggcgacttt
     4741 gtaaggacga ggatacgtct ggcactcaaa acgaacggcg tgtgcaatgg cgcgacaaat
     4801 gtcctgctcc acaacttgca aagacatcgc cgcgtagttt tcactcatgg cctgcgttga
     4861 gtaatagtaa tcatcctgac ttcccgtcat acaagcgata tccgcgtagt ctccgttctg
     4921 cttaatgctt tccagcaaaa ctgacagttc ctcttctgca accgaatagg gtggcgagag
     4981 aaacacggcg cgcgccacca gctgcccagc cgcagaatgc tggcgaatga actctgccag
     5041 gatttcagcc ggttctggtt cggttaccgt atcaacgggg tgacgcatcc cctgtaacca
     5101 ttgccgcttc gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat cccgatggtt
     5161 catctcaccc attccttaca caggcagtag attgtgtgct gccagatcgg cggcaatatc
     5221 ttccagcccc agacgctgca atgtttcgcg ggtcggacaa ccaagctgtg ggtcccagcc
     5281 catttctttg tagaacatcg tgagcgaagc atgcatatcg tcacgatcca ttttgtccgt
     5341 accttcagta aagaccggga tctgcggatc cttgtcgaat acccaggaac agataagatc
     5401 gtgttcatta cgcatatctt tggtttgcat cagttttacc gtgtaggcac gatgcaacgt
     5461 aaagatacgc tctgcagcta aatctaattt ttcctgagtc atctcttcgc cggtgatcgc
     5521 tttgaagaat ttggcttcaa gcgc
//
LOCUS       DACAPB010000241         5542 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.242, whole genome shotgun sequence.
ACCESSION   DACAPB010000241 DACAPB010000000
VERSION     DACAPB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.242"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..407)
                     /locus_tag="IT904_004298"
     CDS             complement(<1..407)
                     /locus_tag="IT904_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP3193988.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVN"
     gene            complement(798..1388)
                     /locus_tag="IT904_004299"
     CDS             complement(798..1388)
                     /locus_tag="IT904_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3193989.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(1609..2376)
                     /gene="hdhA"
                     /locus_tag="IT904_004300"
     CDS             complement(1609..2376)
                     /gene="hdhA"
                     /locus_tag="IT904_004300"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAP3193990.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(2488..3516)
                     /locus_tag="IT904_004301"
     CDS             complement(2488..3516)
                     /locus_tag="IT904_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAP3193991.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            3691..5283
                     /locus_tag="IT904_004302"
     CDS             3691..5283
                     /locus_tag="IT904_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAP3193992.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            5293..>5542
                     /locus_tag="IT904_004303"
     CDS             5293..>5542
                     /locus_tag="IT904_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3193993.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYT"
BASE COUNT         1341 a         1433 c         1321 g         1447 t
ORIGIN      
        1 ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg
       61 gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact
      121 ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc
      181 ggtataaaga cttcgcgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg
      241 gcgaactgat cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt
      301 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg
      361 tcgagttttt tgatttcacg ggttggggtt tctacaggac gtaacataag ggactcctca
      421 ttaagataat aatactggtc aacctttaat ctgattagtt aaccaattct gtgttgcaca
      481 cagttatctg tgagaaaccc atggaagaga aatggaaata aaaagaactg acaccgcgat
      541 caaaaaacca aaaactgcaa tacccacagc gtgatataga tcgcattaat ctttaaaaca
      601 gcgttgacag aagccataaa ataagcaacc gaacgttcgt tcgagcgtat tgggaacgat
      661 aaactctcat gacatatggc tacagtgaat attttggggg gaattctccg aagccgggaa
      721 ggataatttt tgcacagaga aattacgata tgaaacgcgg gcacagttat caccagcccg
      781 ccgaaaaaga gagagaatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc
      841 tgagcaaacg acgcttcacg cgccaggtct tccgcttcga tatcggccag tacaccgtag
      901 gtcagatcca gcagtaaacg tgaagtcatt gccgtgttga tgtctgggct tatctcgcct
      961 ttttgctggg catcacgcat ccgctgggca acaaattccg tgatcgtcat atgcttattt
     1021 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc aaccaccagc
     1081 gcccgttgtc caaaggcttc atgggtgaga gagacaatgg actcgaccat atagtcaacg
     1141 aaatgaatcc cttcaatcgg ttcccggaaa cgggccagcg ccctctcctg gtcctgtaag
     1201 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
     1261 atggcgcaag atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
     1321 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
     1381 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
     1441 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatactt ttcactctga
     1501 aattggttaa ccaatagccg tgatttggct gccagttatt tagcagagac gttgatgagt
     1561 ttatcccggt aaacaacacg cacggttgtt taccggtccg ttagtgtatt aattgagctc
     1621 ctgtacccca ccaccggaga cggtgagaat ttgtccgctt acccagctcg cagcaggcga
     1681 gcaaaggaac agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt
     1741 gtgctgtaac attttttgtt caatttctgg tgtaataacg gatttcaggg catcggttaa
     1801 tattgccccc ggcgcaatgc catttacccg aatatttttt tcacccaggt caaacgccat
     1861 atttctgacc agatgactgg ccgcagcttt agatgatgca taggaagtca tgtttatatt
     1921 tttattttct gccgccatag aagtgatggt cagaataacg ccaccgccat ttttttccat
     1981 ttctggcgca acaagttgtg acagatggaa aaaagaaaac acattcagtt cataagcacg
     2041 gcgaaaatcc gccattggca tatcaaacgg tttaggtcca ccgccaccgg cgttgttaac
     2101 cagaatatca actttaccca gcttactgat agcaaagtct gccagtgcag agagttcctg
     2161 ttcggaagta atatcacaac ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc
     2221 tacaacatgg ttagctgcgt cggcgttaat atcactgacc accacagatg cgccagctgt
     2281 cgcgaatgta attgcgattt ctttaccaat acctgcaccc gcacctgtga tgatggcgca
     2341 ttttccgtcg agtctcaggt tgtcagaatt aaacacataa acctcctgtg aacttcagta
     2401 aaaataaaag gcaccgcctg tgtgccacat tttttatcat taaccttgag gaaaggacac
     2461 taccaggtat agcagataaa gagaggatta tttcgctgca atgagccggg cgggaataat
     2521 aagattgcgt gaatgcgtct cttcatgggt gattttttgc atcatgcgat ccgcaagtgt
     2581 gataccaagt tcccgcgctg gcgtgctggc ccaggtaaca ggtatatcat caagtgtggt
     2641 tggtgtcgca tcggtaaatg ccgccagcga aacctgttgc tcaaagtaac gatcgactcc
     2701 gctttccccg ctttgccgcc ctgctttgag caaaccaaac catgccccca tcgcaatagt
     2761 ttcgttatag caaaccacgg cactgatggt cgggttatga cgtaaaagcg ccgtgatagc
     2821 ttccgcggct tgcttctggc tggaagtgca ctccaacacc caatcgctat gaaacggcag
     2881 gccaaatttt agtagagttg cacaatagcc cccaacccgt tctgcacggg ttaatgagga
     2941 actttgccct cccagccagg cgatccgctg atgcccattg cgaatgagat gctccgtcaa
     3001 caactgtgca gcctgcatgt tgtccgggcg aaccgtatca acatcatcaa gataactggc
     3061 acgggaagcg aaaatcaccg ggatagcttt ttcttctgcc atccgtcgca ggtcatcgct
     3121 acttcctgca gctccggcaa ttaccacacc atcgacaccc tgattcagta acagtgaaaa
     3181 ccgctgtgcc agctgctcac cgtctttacc gccgtgaagc aaaaaaacca tccgtccctg
     3241 cgcttccaga gcttccgtca atccggccgt caattcggcg taaaacggcg cagataaatc
     3301 acggacgatc aaaccaatga cgccgctttg cccgccgcgc agcgccgacg cctggcgatt
     3361 gcgcacaaat cccagctctt caatggcggc gttaacgcgt tctcctgtgg cggtagagat
     3421 tcgccctttg ccactaagca ccagcgaaac ggtacttacc gacacgcccg cagccagcgc
     3481 aacatcatga atggttattt ttttggcggt agccatgcag atgacctact ccctgattat
     3541 gtgacagata aaacgtttta ccttttattt atcttatacc cgctattatc gttgcgtaat
     3601 gtgatttatg cctcactaaa atttgataaa acgttttatc ttctcgcgca atttactgaa
     3661 tccagattgt tctctacgag gagtcgtttt atgacggcga aaacagcacc gaaagtcacg
     3721 ctgtgggagt tcttccagca gttaggcaaa accttcatgt tacccgtggc attattgtcg
     3781 ttctgcggca ttatgctcgg cattggtagt tctcttagca gccatgatgt cataaccctg
     3841 atcccggtcc tgggcaaccc cgtgttgcag gctatcttta cctggatgag taagattggc
     3901 tcgtttgctt ttagtttcct gcctgtcatg ttctgtatcg ccatcccgct gggcctggca
     3961 cgcgaaaata aaggcgtagc ggcattcgct ggcttcgtcg gttatgcggt aatgaacctc
     4021 gcggtaaact tctggttgac caataaaggc attctgccaa ccacggatgc cgcggttctg
     4081 aaagccaata acatccagag cattcttggg atccagtcga tcgataccgg gatcctcggt
     4141 gcggtgatcg ccggtattat cgtctggatg ctgcatgagc gtttccataa tatccgcctg
     4201 ccggatgcgc tggcattctt cggcggtacg cgcttcgtac caattatctc ctcgctggtg
     4261 atgggccttg tcggcctggt gattccatta gtctggccga ttttcgccat gggtattagc
     4321 ggcttgggcc atatgataaa cagcgcgggt gatttcggac cgatgctgtt tggtaccggt
     4381 gaacgtctgc tgttgccgtt tggtctgcat cacattctgg tggcattaat tcgctttacc
     4441 gacgcaggcg gcacgcagga agtctgcgga caaaccgtca gcggcgcact gaccatcttc
     4501 caggcgcaat tgagttgccc gaccactcac ggtttttctg aaagcgccac gcgtttcctt
     4561 tcgcaaggta aaatgcctgc gtttctcggc ggtctgccag gtgcagcgtt agctatgtat
     4621 cactgcgcgc gcccggaaaa tcgccataaa attaaaggtc tgctgatttc tggcctgatc
     4681 gcctgcgtcg ttggcggcac taccgaaccg ctggaattcc tgttcctgtt cgtagcgcca
     4741 gttctgtatg tcatccacgc gctgttaacc ggcctcggct tcaccgtcat gtctgtgctc
     4801 ggcgtcacca tcggtaatac cgacggcaat atcatcgact tcgtggtgtt cggtattttg
     4861 catggtctgt caaccaagtg gtacatggtg ccagtggtgg cggcaatctg gtttgtcgtt
     4921 tactacgtca tcttccgttt cgctatcacc cgcttcaatc tgaaaacccc ggggcgcgat
     4981 agcgaagttg ccagctcaat cgaaaaagcc gttgccggtg cgccgggtaa atcaggttac
     5041 aacgttcctg caatcctcga agcattaggc ggtgccgaca atattgtcag cctcgataac
     5101 tgcattaccc gtctgcgttt gtctgtgaaa gatatgtcgc ttgttaatgt gcaggcactg
     5161 aaggacaatc gggcaattgg cgtagtacaa cttaatcaac ataacctgca ggttgttatc
     5221 gggccacaag ttcagtcagt aaaagatgaa atggccggtc tgatgcatac tgtccaggca
     5281 taaggataag atatgttcga tttttcaaag gtcgtggatc gtcatggcac atggtgtaca
     5341 cagtgggatt atgtcgctga ccgtttcggc actgctgacc tgttaccgtt cacgatttca
     5401 gacatggatt ttgccactgc cccctgcatt atcgaggcgc tgaatcagcg cctgatgcac
     5461 ggcgtatttg gctacagccg ctggaaaaac gatgagtttc tcgcggctat tgcccactgg
     5521 ttttccaccc agcattacac cg
//
LOCUS       DACAPB010000242         5523 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.243, whole genome shotgun sequence.
ACCESSION   DACAPB010000242 DACAPB010000000
VERSION     DACAPB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.243"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1604
                     /gene="prlC"
                     /locus_tag="IT904_004304"
     CDS             <1..1604
                     /gene="prlC"
                     /locus_tag="IT904_004304"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAP3193994.1"
                     /translation="KQQRYGEIATRLSELGNQYSNNVLDATMGWTKLVTDEAELAGMP
                     ESALAAAKAQAEAKELEGYLLTLDIPSYLPVMTYCDNQALREEMYRAYSTRASDQGPN
                     AGKWDNSKVMEEILALRHELAQLLGFENYAFKSLATKMAENPQQVLDFLTDLAKRARP
                     QGEKELAQLRAFAKAEFGVDELQPWDIAYYSEKQKQHLYSISDEQLRPYFPENKAVNG
                     LFEVVKRIYGITAKERKDVDVWHPDVRFFELYDENNELRGSFYLDLYARENKRGGAWM
                     DDCVGQMRKADGSLQKPVAYLTCNFNRPVNGKPALFTHDEVITLFHEFGHGLHHMLTR
                     IETAGVSGISGVPWDAVELPSQFMENWCWEPEALAFISGHYETGEPLPKELLDKMLAA
                     KNYQAALFILRQLEFGLFDFRLHAEFRPDQGAKILETLAEIKKLVAVVPSPSWGRFPH
                     AFSHIFAGGYAAGYYSYLWADVLAADAFSRFEEEGIFNRETGQSFLDNILSRGGSEEP
                     MDLFKRFRGREPQLDAMLEHYGIKG"
     gene            1612..2364
                     /gene="rsmJ"
                     /locus_tag="IT904_004305"
     CDS             1612..2364
                     /gene="rsmJ"
                     /locus_tag="IT904_004305"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAP3193995.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(2413..3882)
                     /gene="dtpB"
                     /locus_tag="IT904_004306"
     CDS             complement(2413..3882)
                     /gene="dtpB"
                     /locus_tag="IT904_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAP3193996.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(4200..4634)
                     /gene="uspA"
                     /locus_tag="IT904_004307"
     CDS             complement(4200..4634)
                     /gene="uspA"
                     /locus_tag="IT904_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAP3193997.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            5025..5360
                     /gene="uspB"
                     /locus_tag="IT904_004308"
     CDS             5025..5360
                     /gene="uspB"
                     /locus_tag="IT904_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAP3193998.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
BASE COUNT         1402 a         1376 c         1495 g         1250 t
ORIGIN      
        1 agaaacagca gcgttacggc gaaattgcga cccgtctttc tgaactgggc aaccagtaca
       61 gcaacaacgt cctcgatgcg acaatgggct ggaccaaact cgttaccgac gaagcggagc
      121 tggcggggat gccagaaagc gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc
      181 tggaaggtta tttgctgacg ctggatatcc caagctactt gccggtaatg acctactgcg
      241 acaaccaggc tctgcgtgaa gagatgtatc gcgcttacag cacccgcgcc tccgatcaag
      301 gcccaaacgc cggtaaatgg gataacagca aggtgatgga agagatcctc gcgctgcgtc
      361 acgaactggc gcaactgctg ggctttgaaa actacgcctt taaatccctt gccactaaaa
      421 tggcagaaaa cccgcagcag gtgctggatt tcttaaccga tctggcaaaa cgcgcgcgtc
      481 cacaaggcga aaaagagctg gcgcaattgc gtgcctttgc caaagccgaa tttggcgtcg
      541 atgagttgca gccgtgggat atcgcttact acagcgaaaa acaaaaacag cacctctaca
      601 gcatcagtga cgaacagctg cgtccgtact tcccggaaaa caaagcggtt aacggcctgt
      661 ttgaagtggt taagcgtatt tacggcatca ccgctaaaga gcgtaaagat gttgatgtct
      721 ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa taacgaactg cgcggtagct
      781 tctacctcga tctgtatgcc cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg
      841 taggccagat gcgtaaagct gatggttctt tgcaaaaacc ggtcgcgtat ttgacttgta
      901 acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac tcacgacgaa gtgatcaccc
      961 tcttccacga gttcggtcac ggcctgcacc atatgctgac ccgcatcgaa accgctggtg
     1021 tttccggtat cagcggtgtg ccgtgggatg cggtcgaact gccgagtcag tttatggaaa
     1081 actggtgctg ggagccggag gcgctggcgt ttatctctgg tcactatgaa accggcgaac
     1141 cgctgccgaa agagttgctg gataaaatgc tggcggcgaa gaactaccag gcggcgctgt
     1201 ttattctgcg tcagctggag ttcggcctgt ttgatttccg ccttcatgcc gagttccgcc
     1261 cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat caagaaactg gttgccgtgg
     1321 tgccatctcc gtcctggggc cgtttcccgc acgctttcag ccatattttc gccggtggtt
     1381 atgccgcagg ttactacagc tacctgtggg ctgacgtact ggcggcagat gctttctcgc
     1441 gctttgagga agagggcatt ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc
     1501 tgagccgtgg cggttcagaa gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac
     1561 cgcagctgga tgcgatgctg gagcattacg gcattaaggg ctgatcattc agtgaaaatc
     1621 tgcttaattg atgaaacagg caccggagac ggtgccttat ctgttctggc ggcccgctgg
     1681 gggctggagc acgatgaaga caacctgatg gcgctggtgt taacgccgga acatctggaa
     1741 ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg ttgattttgt tggcggagcg
     1801 atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc
     1861 attaaaggcg attatttgcc ggatgtggtg gatgccactg caggacttgg gcgcgatgcc
     1921 tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg agcgtaatcc agtggttgcc
     1981 gcgctactcg acgacggcct ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg
     2041 caggagcggt tgcagttaat tcacgcctcc agcctgacgg cgctgactga tattaccccg
     2101 cgcccgcagg tggtttatct cgacccgatg ttcccgcata agcagaaaag cgcgctagtg
     2161 aaaaaagaga tgcgtgtctt tcagtcgctg gtgggaccgg atcttgatgc cgatggatta
     2221 ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg
     2281 ccaccgctgg cgaatgtcgc cacgccaaac gcggtagtca ctaaagggca tcgctttgat
     2341 atctatgcag gtacgccagt gtaaaactaa aacagggtag cgataacgct accctgcttt
     2401 ctgcaagaat aattaatggc tttccggcgt cgcaatcatg cgtttcagcc acggcaccat
     2461 caacagcatc actactgcaa cgcccagcgt gaccagacca atcttaccaa acacgttggt
     2521 atagacgggc aacgtctcaa gcggatcggt aatgttgtcc ggcaccgcgg taaatgttgc
     2581 cacatagccg cccagcaaga acgcggcagc ctgcgtcagg aaccacatcc cgagaataaa
     2641 gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc aggccaaggg cgctaataaa
     2701 cagttcacct aagctctgga ataagtacac cagcacgata aaccatggcg atgtcagccc
     2761 ttgtgcatcc gcaaaccaca ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat
     2821 aaacatgccg agagtaaatt tcatcggcat cgagaggtct ttgcctttgt tacccagatg
     2881 cgtgtaaatg cctgccagta ttgggctggc gagtaccacc cagaacgggt taagcgcctg
     2941 gaagctgacc gggttgatgg aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa
     3001 gaagttcagc gatgttggca tctgggcgta gagaatgtaa aacaccaccg cttcgagcat
     3061 caggacaaag gcgacaaaca ttttattgcg cccggtttta tccagcttga atgcctgacg
     3121 aaagaagatg atggtgacga cgatggagag aacaatcagc accagattgg cgacttctac
     3181 gttgtgcatc agccatgcgc atacgaagat catcaccacg ctgccaagta acacgtacaa
     3241 cagtttgctg aagctcattg gccggaagtc gggttcagaa ccaatgtctt tcaccattcc
     3301 acgacaggcg atgtaaacca gtaatgcgat aattaacccc gccccgcaca ggttgtaggt
     3361 gactgaataa ccgaatctat cagcgatcac aggggccagc gataacgcta tcaacgagcc
     3421 gatgttgatc gacatataga acagggtgaa tgcgccatca agccgcggat ctttcggcgg
     3481 atagcacttc gaaagcaagc tggctgggtt agctttaaac aggccgttac ctacagcgat
     3541 agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc gacatgccgg tcatgaagta
     3601 gccaatcgcc agcacaagtg caccaagaac aatggtgcgt ttggtcccca gcaggtggtc
     3661 gccgacatag ccgccaatgg aaatgaggcc atagaccagc gcagcaaaag caccaaaagt
     3721 gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc
     3781 ctgcacgccg tagtagccga atcgctccca taactcgaca aaaaagatca tgaaaaatgg
     3841 gcgaggttgc tgcagcatcc ccatgggtgt tgttgtattc atatttatta accttccaat
     3901 accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag
     3961 agattcttaa tattgatgcg gtgaatagat taatacacta ttacaacaga aaataaccag
     4021 ctgatcacac taatgatgga aaatatgatg gtgttaaata ttgtattggc tggggatttt
     4081 gattgcgaac gcagaccgta ggccagataa ggtgtttacg ctggatcagg taacaaggcg
     4141 cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgta gagggaagat
     4201 tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt gttgatcagc
     4261 tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca aaccaccaga
     4321 tccatatcgt atttcttgat tgcatcgacc agaacttggc ccaggtcgcc gctgccgctc
     4381 agggtttcag tgattgggta gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc
     4441 tcttctgaga tgcgtttctg catatcaccc agattcacat caataagccc ggtgtatagg
     4501 tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg gcgagccata
     4561 gagactgctt tctctaccag aactttgctt tccggggaga gatcgaccgc gatgagaatg
     4621 tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc cagctagcgt
     4681 ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct aacccagccg
     4741 taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc cgtcaatccg
     4801 ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag tggttaacct
     4861 tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg atgttggtca
     4921 ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat cgggagcggt
     4981 agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata agcaccgtcg
     5041 cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac
     5101 tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa tatgttgatg
     5161 gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc gtttggtata
     5221 tctatgccca acgttatcgc gatcatcacg atgatgaatt tattcgccgc tgtgagcggg
     5281 tgcgtcggca gtttattctg accagcgcat tgtgtggtct ggtggtggtc agcctgattg
     5341 cattgatgat ttggcattaa gcacttcagc aataataacg tataaaaaaa gcgggccagt
     5401 aaactgaccc gctcgagact gattacaaac gcaaaattgc ctgatgcgct acgcttatca
     5461 ggcctacata atctctgcaa tctattgaat ttgcatgttt ttgtaggccg gataaggcgt
     5521 tca
//
LOCUS       DACAPB010000243         5519 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.244, whole genome shotgun sequence.
ACCESSION   DACAPB010000243 DACAPB010000000
VERSION     DACAPB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.244"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..178)
                     /locus_tag="IT904_004309"
     CDS             complement(<1..178)
                     /locus_tag="IT904_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP3193999.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKAR"
     gene            complement(249..2363)
                     /gene="fusA"
                     /locus_tag="IT904_004310"
     CDS             complement(249..2363)
                     /gene="fusA"
                     /locus_tag="IT904_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP3194000.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADIVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2460..2930)
                     /gene="rpsG"
                     /locus_tag="IT904_004311"
     CDS             complement(2460..2930)
                     /gene="rpsG"
                     /locus_tag="IT904_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP3194001.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3027..3401)
                     /gene="rpsL"
                     /locus_tag="IT904_004312"
     CDS             complement(3027..3401)
                     /gene="rpsL"
                     /locus_tag="IT904_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP3194002.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3527..3814)
                     /gene="tusB"
                     /locus_tag="IT904_004313"
     CDS             complement(3527..3814)
                     /gene="tusB"
                     /locus_tag="IT904_004313"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAP3194003.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3822..4181)
                     /gene="tusC"
                     /locus_tag="IT904_004314"
     CDS             complement(3822..4181)
                     /gene="tusC"
                     /locus_tag="IT904_004314"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAP3194004.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4181..4567)
                     /gene="tusD"
                     /locus_tag="IT904_004315"
     CDS             complement(4181..4567)
                     /gene="tusD"
                     /locus_tag="IT904_004315"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAP3194005.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4567..5289)
                     /locus_tag="IT904_004316"
     CDS             complement(4567..5289)
                     /locus_tag="IT904_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP3194006.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
BASE COUNT         1363 a         1422 c         1334 g         1400 t
ORIGIN      
        1 cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca gcaccgccgt
       61 aggttttagc cagtacggtg gtgattgcag cggtcagagt agttttaccg tggtcaacgt
      121 ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct ttagacacgg
      181 ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt tggtattaac
      241 ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact cggcgcttca
      301 tcatacttca ggaattccat agtgtatgat gcacgacctt tggtcagaga acgcagctga
      361 gttgcgtatc cgaacatttc agacagcggt acttcagcgt ggatcttaac gccagtaact
      421 tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat aacgtcaccg
      481 gtgttctctt ccggagtttc tacttcaacc ttcatgatcg gctcaagcag aactggtttc
      541 gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc cagttcagag
      601 gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac taccgggtag
      661 cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc cgggatgtat
      721 tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt cgggtttgaa
      781 cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg accaccagac
      841 tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc acggtaagca
      901 acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg gtcaacgatg
      961 atgtcgaggt gcagttcgcc catacccgca atgatggtct ggttagattc ttcgtcagtc
     1021 catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc cattttttcc
     1081 tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg gaattccata
     1141 cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt tacgtctttc
     1201 agaccgatag cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc acgtttgtta
     1261 gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga gttcagtacg
     1321 gtatcaccag agttaaccac accggagtaa acacggaaga aggtcaggtt accaacaaac
     1381 gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact tgcgtgacgt
     1441 tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac gtcaaccggg
     1501 gatggcaggt aatcgattac cgcatccagc atcgcctgaa cacctttgtt cttgaacgca
     1561 gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag agcacctttg
     1621 atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc ttcagaagct
     1681 tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac tatgtctgcc
     1741 gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt gatagctttc
     1801 attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc cagctgcagc
     1861 ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag gaagttcgca
     1921 cccatgcggt ccattttgtt aacgaacgca atgcgcggaa ctttatattt gtttgcctga
     1981 cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac cattaccgca
     2041 ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg ccccggggtg
     2101 tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca gaatgcagta
     2161 gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca gtccatggtt
     2221 gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa cagaatacgt
     2281 tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt acggtagcgt
     2341 gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt tcaatttaag
     2401 tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact ccgaagggat
     2461 taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac gtcttcacgt
     2521 ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc caggcgcaga
     2581 gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat tgccagagca
     2641 ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc aacgcggcga
     2701 gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc ttccagttca
     2761 gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga ttcagcagta
     2821 gattttttac catctaccat caggatattt acaaatttag ccagcagttc tgatccgaac
     2881 ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat ggaaatactc
     2941 cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt aagttaaaac
     3001 gtttggcctt acttaacgga gaaccattaa gccttaggac gcttcacgcc atacttggaa
     3061 cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac ggtgtggtaa
     3121 cgaacacccg ggaggtcttt aacacgaccg ccacggatca ggatcacgga gtgctcctgc
     3181 aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt cagacgaaca
     3241 cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata tacacgagta
     3301 catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt cgcaactttg
     3361 cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct cctggtttta
     3421 gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac gccgaatttt
     3481 agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac caggccatct
     3541 ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggga atgatgtcgt
     3601 tcgaaatttg accggtcaaa ccgcgggcaa taaggtcttc gttcagggca tagaccttaa
     3661 tgggggcatt acgcagactt tcaaggtagc ggttaccgtc aactgcggca gttacgccat
     3721 cttgcaataa tagcagttcg tctccttcac tgagcagacg cagcagcgca gcaaaatccg
     3781 tcagccaggg tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct caaaataaca
     3841 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgacaaca
     3901 aagggtgtct gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac ccagcactgt
     3961 tcaatgtcgt acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4021 tcgggctttt gtcctgacag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4081 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccgacc agcagctgta
     4141 ccatgaggtg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4201 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4261 ggtttgacga agccagcccc agccttcctg cttcagtttc atcgacgata ccgcggcgta
     4321 acgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4381 cgcgcacaag gtcaaattcg tcacttgccg gtgaggtcaa ttggttagcg ttatagaccc
     4441 cttcccgata gaaaaagacg ctgcttaact catgaccttc tactatcagc gcctgcgcaa
     4501 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4561 aacgcattac ttatcttgcc cctggaaatc accgcttttg aactggcgga tgtagagata
     4621 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc
     4681 gaagatccct ttctcgtaga gattcagcac aatctgacga tttttggcgt tattagaaac
     4741 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4801 aacagaagag gcaaagttga cgcttgaacc gacatccggg gtttctggcg gcacaaaagt
     4861 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     4921 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     4981 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5041 tagcgccagg tcagtaattg gcgaaccaat cttccggccg gtatgttcac cgttagcaat
     5101 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5161 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5221 ctggtcgaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5281 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5341 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5401 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcgattctt aatgcttatt
     5461 ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgct
//
LOCUS       DACAPB010000244         5502 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.245, whole genome shotgun sequence.
ACCESSION   DACAPB010000244 DACAPB010000000
VERSION     DACAPB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.245"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(18..791)
                     /gene="uxuR"
                     /locus_tag="IT904_004317"
     CDS             complement(18..791)
                     /gene="uxuR"
                     /locus_tag="IT904_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAP3194007.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1006..2466)
                     /locus_tag="IT904_004318"
     CDS             complement(1006..2466)
                     /locus_tag="IT904_004318"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP3194008.1"
                     /translation="MTTIVDSNLPVARPSWVHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2547..3731)
                     /gene="uxuA"
                     /locus_tag="IT904_004319"
     CDS             complement(2547..3731)
                     /gene="uxuA"
                     /locus_tag="IT904_004319"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP3194009.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4071..5414
                     /gene="gntP"
                     /locus_tag="IT904_004320"
     CDS             4071..5414
                     /gene="gntP"
                     /locus_tag="IT904_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAP3194010.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
BASE COUNT         1191 a         1491 c         1470 g         1350 t
ORIGIN      
        1 gcgcttatta taataagtca ggcgtcgact ttatccagcg gccatgaatc aaacagatag
       61 ccatcaaaat aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc
      121 agatgctgcc acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg
      181 atctgtttgt gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc
      241 aactggatcc acattggatt gttttcccgc cactgccagg actgacggaa cagctccacc
      301 agcatgctgt tatgcgttgc ttcggcaata gcgagatgga actgcatgtc accgctttcg
      361 ctgctgcccg gcgcactgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt
      421 ttgacgatat cttcgcgggt agcctgcagg gcggcaaact cggcgatgtt gctctcaaga
      481 agctgtcgcg cctgcaacag ctcaaaagga cccgcatcat tgcaaacgtt ggcatccgga
      541 ctgtctgtgt tgtggctgcc tgagctatca agaacataga taccggcacc ccggcgcact
      601 tccaccagcc ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca
      661 tcaagcattt ctgcaatttc acgctccggc ggcaaccgtt cgccaggatt gtacggcgtc
      721 tttatgatca gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg
      781 gcagatttca tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc
      841 gtgaacgcct tatccggcct acaacgggca attttacggc acccgttaat ctcaaactat
      901 accattagca ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca
      961 aatatcagtt tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac
     1021 acactcgcgc gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa
     1081 gtcggcattc tgcggcagat catcggcaaa aataccgctc aggcccagca gcgctttcac
     1141 gcggtctgcg ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac
     1201 cacgtcaatg gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac
     1261 gcccagcgcc aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccggatccag
     1321 cagacgctgc ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag
     1381 agacgggttg ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc
     1441 cggcatcgac agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata
     1501 atccgggtta gtcatggtgt cggcaatagt ttcatagccg ccgaggtaac cgaggtatgc
     1561 caggaaagag tggctgccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc
     1621 tgcaacgaac tgtgcgccca ctttatccca gtccggacga ccattaacga agttatcttc
     1681 aatcacccac tgacggaacg gttcgcaggc aatggcgcac gggtcataaa cacccagctg
     1741 gtcagcaatt tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt
     1801 gcacgggaag gtgacgttct cttcaatcca tgccgccagc tgcgggtcac gcgcctgagc
     1861 cagccccagt accgcgacct tcgccacatg accgttttca cgcacgttat cgcaggacat
     1921 caccgtaaac gctttcagcc ctttttcacg acgcagacgc agggcttcga cgatgtaacc
     1981 aatcgcagac ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag
     2041 atccaactga ccgcttgccg catcagcgca gtagcctttt tccgtgaccg tcagagagac
     2101 aatcgccgtc tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg
     2161 atgcagcgct tctttcatcg aaccgataat tttcagttcg gtgctttccg cgcctttttc
     2221 cgccacggtg tacagcagtt gctgtttttt caggttttca atcagcacac ggtcgttacc
     2281 tggcatcagg ttaacttcgc agatacccca gtcgctgtcg gtgctttcca gcagatgatg
     2341 ggtatacagc gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga
     2401 ttccagacga gaatgcaccc atgacgggcg ggcaaccggc agattgctgt caacaatagt
     2461 agtcatcgta aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc
     2521 acggcgcgca gccatgccgg tggatattaa cggctaaaga aagcgcgctg gatcgccagt
     2581 tcgacaccgc gaacttcggc caggcctttc agacgaccaa ttgcggagta acctgggtta
     2641 gttttcttct tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg
     2701 tcttctttgc cttccgcttt acgacggtgt tcctcttcaa caatcgcttt caccacttcg
     2761 tacatatcaa cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca
     2821 cgcatggtgg agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca
     2881 accagatcgt tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc
     2941 atgctgttta cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc
     3001 aggccgagga tcgggcgcgg cggatcgtcc gggtgaacag ccatacggac gccaacttct
     3061 tcggcaaccg gaataatcgc tttcaggaag acggcaaagt tttcgcgcag tttggctttg
     3121 tcgatatctt tatatagctc caggtgctta cggaactggt cgagggtgta cccttcttcc
     3181 gcgcccggaa gaccagcaat gatgttacgg gtcagacgcg ctttgtcttc gtcgctcata
     3241 gtggcgaagc gaacagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct
     3301 ggacgtttca ggatatgcat ttcgaatgca gcgaattcga tctggtcaaa gcgcagagct
     3361 ttggagccgt ctggcagcac gtattcgagg tcagtacggg tccagtcaag caccggcatg
     3421 aagttgtagc acacggtgcg aatgccgcac tgcgccaggt tgcgcagagt ctgctgatag
     3481 ttagcgatcc actgctcata gttgccagtg tgggttttga tatcttcgtg aattggcacg
     3541 ctttctacga cagaccatac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc
     3601 tcttcaacgg accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt
     3661 gcgcccgcct gacggacatc agctaaagaa accggatcgt ttgggccgta ccagcgccag
     3721 gtctgttcca tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac
     3781 cctggagttg tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga
     3841 tgagatggcg tataagttct accttttggt aatacatgtc aatccgaatt ctaaaattgg
     3901 ttaaccacat cacaagaatt tcactctcct ggttgttgct gcaaattgca gcaaagggta
     3961 atcgatggtt attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca
     4021 taacatgtcg cccaaaataa caacggttca accaggtaag gtaaaacgtt atgcatgtgc
     4081 ttaacattct ctgggtggta ttcggcattg gtctgatgct ggtactgaat ttgaagttca
     4141 aaatcaattc aatggtggct ttgttggtgg cggcgctgtc cgtcgggatg ctggcgggca
     4201 tggatttgat gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggggaac
     4261 tggctatcat cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg
     4321 ctcaccagat agcacatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg
     4381 tgattatcat cggcctgatt tttggtctgg cgatgttcta tgaagtggcc tttatcatgt
     4441 tagcgccgct ggttattgtc attgccgccg aagctaaaat tccgttcctg aaactggcga
     4501 tcccggcagt agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtcctg
     4561 tggcgctggt gaatgcttat ggcgcagata tggggatggt ttatatctat ggcgtactgg
     4621 tgacgatccc aagtgtaatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg
     4681 agcgcccaac gccatcattc ctgaaagcag atcaaccggt agatatgaac aatctgccct
     4741 ctttcggcgt ttcgattctg gtgccgctga tcccggcgat cattatgatc tccaccacca
     4801 tcgccaatat ctggctggta aaagataccc ctgcctggga agtggttaac tttatcggtt
     4861 cctcgccgat tgcaatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcac
     4921 gtggtcatga catgcagtgg gtgatgaacg cttttgaaag tgcggtgaaa agcattgcaa
     4981 tggtgattct gatcatcggt gcgggtggcg tgttgaagca aactatcatc gacaccggca
     5041 ttggcgacac catcggcatg ttgatgtccc acggcaatat ctcgccctac attatggcat
     5101 ggctgatcac tgtgctaatt cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg
     5161 ccgccgggat tatcagtgct gcaatccttg atccagcaac cggtcagctg gttggcgtga
     5221 atccggcgct gctggtgctg gcgacggctg cgggttccaa caccctcacc cacattaatg
     5281 atgcctcatt ctggctgttc aaaggttact ttgacctgtc ggtaaaagac acgttgaaaa
     5341 cctggggact gctggagctg gtcaactccg tggttgggct gattattgtg ttgattatca
     5401 gcatggtagc gtaaaagaac accagagcct gccaatggcg ggctctgttc ggattgtcgg
     5461 caaagtgcgc tttgtttatg ccagatgcga cgctgacgcg tc
//
LOCUS       DACAPB010000245         5390 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.246, whole genome shotgun sequence.
ACCESSION   DACAPB010000245 DACAPB010000000
VERSION     DACAPB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.246"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(122..769)
                     /gene="gloC"
                     /locus_tag="IT904_004321"
     CDS             complement(122..769)
                     /gene="gloC"
                     /locus_tag="IT904_004321"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAP3194011.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(796..1344)
                     /locus_tag="IT904_004322"
     CDS             complement(796..1344)
                     /locus_tag="IT904_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAP3194012.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1525..3372)
                     /gene="ldtD"
                     /locus_tag="IT904_004323"
     CDS             complement(1525..3372)
                     /gene="ldtD"
                     /locus_tag="IT904_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP3194013.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLCDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3633..>5390)
                     /gene="mukB"
                     /locus_tag="IT904_004324"
     CDS             complement(3633..>5390)
                     /gene="mukB"
                     /locus_tag="IT904_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAP3194014.1"
                     /translation="LDEAQEAARFVQQFGNQLAKLEPIVSVLQSDPEQFEQLKEDYAY
                     SQQMQRDARQQAFALTEVVQRRAHFSYSDSAEMLSGNSDLNEKLRERLEQAEAERTRA
                     REALRGHAAQLSQYNQVLASLKSSYDTKKELLNDLQRELQDIGVRADSGAEERARIRR
                     DELHAQLSNNRSRRNQLEKALTFCEAEMDNLTRKLRKLERDYFEMREQVVTAKAVWCA
                     VMRMVKDNGVERRLHRRELAYLSADDLRSMSDKALGALRLAVADNEHLRDVLRMSEDP
                     KRPERKIQFFVAVYQHLRERIRQDIIRTDDPVEAIEQMEIELSRLTEELTSREQKLAI
                     SSRSVANIIRKTIQREQNRIRMLNQGLQNVSFGQVNSVRLNVNVRETHAMLLDVLSEQ
                     HEQHQDLFNSNRLTFSEALAKLYQRLNPQIDMGQRTPQTIGEELLDYRNYLEMEVEVN
                     RGSDGWLRAESGALSTGEAIGTGMSILVMVVQSWEDESRRLRGKDISPCRLLFLDEAA
                     RLDARSIATLFELCERLQMQLIIAAPENISPEKGTTYKLVRKVFQNTEHVHVVGLRGF
                     APQLPETLPGTDEAPSQAS"
BASE COUNT         1250 a         1469 c         1342 g         1329 t
ORIGIN      
        1 aatattttga atttgcacga ttttgtaggc cggataaggc gctcgtgccg catccggcat
       61 aaacaaagcg cactttgcca gaaatccagg cccgcattaa aagcgggctt ttttatgtgc
      121 cttaccagac gggcatttcg tcttgcagga agggattatg caggcgttca taaccaagtg
      181 tggataatgg tccgtgaccc ggaataaatg tcacgtcatc ccccagtggc agcaatttat
      241 ctttgattga agaaatcagt tgattatgat cgccacgcgg gaagtcactg cgccctactc
      301 cgcctttgaa aataacatcg ccagaaatca gcagctttgc ccgatcatca aaaaacacta
      361 catgacccgg cgtatgccca gggcaatgta acacctgtaa agtcacattc cctatgctga
      421 tggtatcgcc ttcgttcagc caacgatctg gcgtcagcgg ctggcactct tccagaccaa
      481 acatacgact ttgcgcaggc aagccttgca gccagaactc atcttctttt tccgggccaa
      541 aaactggcac gccgtaatgt tgcgccagtt ccgccgctgc gccaacgtgg tccagatgac
      601 catgcgtcag caggatctgc atcagtgtca ggccgctgtc atcaacttcc tgtttgattt
      661 tttccgcatc gccgccagga tcgaccagtg cggccagacg ggtttgttca caccagatta
      721 atgaacagtt ctgggagaat gcggtgaccg gaataatacg atagttcata ctgcccctgt
      781 ttcgttaagc gattgctacc agtgccgtgc tggcccggta tcaatatgca caaagttact
      841 acgtgggtaa tatcctacac cacctgcgcg catagataac gcggctttgc gaatattgct
      901 taacgcgata ccttcaatat ggaaatccat cgcctggcct ttagtgtgat agcttttctt
      961 cgctactcca cggctgcggg cgcgtagttc attgttggta tcaatagaac gataaccgga
     1021 aatgagttgc accggtttgc gcgtgcctaa caacccttgc aggcgataca actggtcgaa
     1081 taatcctggg tcgatggact ttattttgtt cgcgcggtaa tcgcggaaaa aatggttaag
     1141 ttttgccaat tcttcctgaa tatagcctct gccatcgaaa aactccgctt tgattgactc
     1201 tccggtatga agattattga gtgtcaaaat gcgcgggcgt ggggtagaga gtgttgcaaa
     1261 cgcaggggtc ggcaggatgg cggcaccgag tgcaacgcca ccaagcgcca gcaatttgcg
     1321 gcgattagcg tcgaatttgt ccatgataat caagtctaca ggtcaatgtt atcgtttata
     1381 tgcacttctg gcgcacaaga ggcaccttaa ccgcccgtaa agcagacgtc aagatgactc
     1441 aaccccagca gtgacggggg ctgcagagaa tcgcccccaa tcatgctaat tattacgaca
     1501 actgatttcc ccgaactact tcatttacct gattaattgt tccgctttcg atacgatttg
     1561 cgagctggat cgcgcaggca gatcataatt gtaaatatct gtacgatact gggtacgacc
     1621 atctgcacca acaaaggccg tcaggtagta gagattcacc ggaatcgact gccgaatatt
     1681 gacgtaacgt gtatcaccct gcttcagcgc atcagaaata cgtttgtcat tccagcctgc
     1741 atcctgcaac agcatattcg ccagatcgga agctttattc actcgtacac agcctgagct
     1801 caatgcgcgt gtatcacgct tgaacagatt gtggttcggc gtgtcatgca aataaatggc
     1861 ctctgaactc ggcatattga atttatagcg ccccagcgag ttccgtgggc ctggagcctg
     1921 ctggaagcgg aacggtaaat tcgaggccgt gattgtagac cagtcaacct gccatgggtc
     1981 aatcgcttct ctgctgttcc agccgcgcat caccgtatag ccatggcttt cgagatatcc
     2041 cggatcgttg cgcacttttg gcagaatatc tttgcgtgcc agagttggag gtacgttcca
     2101 cggcgggttt accactacgt tgttaagggc actgctcatc atcggcgttt tgcgatcggg
     2161 gcgaccgaca atgactcgcg aatccagcac ctgattgccg ttctgatagt agaccagcga
     2221 ataggccgga atgttaacca tgatcccggt agaaagctct gttggcagca agcgcaatcg
     2281 ctggatgttg agagccaaca cgccagcacg ctgggcgggc gttacgttta accagtcacg
     2341 tgttgccggg ccaatagcac catctgcccc caatccttgc catgcctgaa aacgtttaac
     2401 ggcttccacc agttcattat cgtaggcggc gcgaacggca ggcgcaggtt tactacgaga
     2461 cgtcgtttgc ttatccatcg gcttagtttc tgctgtttca acagtaacag cggatgggct
     2521 gactaccgcg tcagttggcg tgtcatcgcc aggtagagta attttcggcc ccccgtccaa
     2581 catgcctgtg cgttgcaata tttcgcgcaa cgccggtacg tcgttactcc actgccctgg
     2641 gcgcaacgtt gctttgccgg tcagttgggg ccacggtttg gtgtcacaga gtaaggccag
     2701 taacgattca tgcatcgccg catattgcgg atgctgcggt gccagtcctg caacaaacgt
     2761 aggcaattga cctttatcca gcgccagctg ccattggtta atcaccgaga gcggcggcgt
     2821 tgcaagtgca taaggtttac tgctatatag ccagcgagtg cctttgaccg gaatatttgc
     2881 aatgaaatgg agatagccca tcatcgcatc agagagcacc acgtcgcgtg ccatcccgtt
     2941 aacaccagga tcggtcagta actctaccca tttattaaac tgcggctgga aaccggcaat
     3001 cgccacctct gccagctgtt gctggaaggc tttaacagca tcacggtttt cccacatggg
     3061 ttgcatatcg cgtgcggcat acaacagttg aagctggtta agataaaccg gcttgtaacc
     3121 tgcgggtaat tgagattcga tttgcgtgcg ggctttttct gccgcacctt caggcaatgg
     3181 ctggatcccc gccattattg ccgtggcttg cgcctgcggc agtgcctcgc cctgttcact
     3241 gacagccacc gggctgtcgc cagggattac ttcaggctca tcggcctgcg cattgaacag
     3301 tggagcgaat gttacggcca ggcacaaact gattgccgac agccgacgac cacacatcat
     3361 attaagcaac atcccttgcc ccctgttttt atcatactct gaatagccag gctgttattt
     3421 tacgacttgc ctgtacaacg ccaatactca cgaaagtatg acgagcaagc ctgttactca
     3481 tcaggcttat cttcagtata taaacgccga tacgttttta cataacctaa tgtaaagaag
     3541 tttaaagaaa agcgcgccgt aatgtgccac ttttttagtg cagaaacgga gttttcggaa
     3601 aaagaaaagg cggcattgct gccgccttaa ttttaactcg cctgagaagg cgcttcgtca
     3661 gttcctggaa gcgtttcagg gagttgcggc gcaaatcctc gcaggccgac gacatgaacg
     3721 tgttcggtat tctggaagac tttacgcacc agtttatagg tggtgccttt ctccgggctg
     3781 atattttccg gcgctgcgat gatgagttgc atttgcaaac gctcacacaa ttcaaacagc
     3841 gtggcgatag aacgagcatc cagtcgcgct gcttcatcga ggaacagcag gcggcaagga
     3901 gagatatctt taccgcgcag gcggcgagat tcatcttccc agctttgtac caccatcacc
     3961 agaatcgaca taccggtacc aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc
     4021 agccagccat cggaaccacg gttaacctca acttccattt ccagatagtt gcggtaatcc
     4081 agcagttctt caccgatggt ctgcggcgtg cgctgcccca tatcaatctg cgggttaaga
     4141 cgttgataca gtttcgccag cgcttccgag aaggtcaaac ggttgctgtt aaacagatcc
     4201 tgatgctgct cgtgctgttc agagagcaca tccagtagca tggcgtgcgt ttcacgcacg
     4261 ttcacgttga gacgcacgct gttcacctga ccaaacgata cgttctgcaa cccctggttg
     4321 agcatacgga tacggttctg ctcgcgctga atggttttgc gaatgatgtt cgccacgctg
     4381 cgggaactga tcgccagttt ctgttcacgg gaggttaatt cttcggtcag acggctaagt
     4441 tcaatctcca tctgttcgat agcttccacc ggatcatcgg tacgaataat atcctgacga
     4501 atacgttcac gcagatgctg ataaaccgcc acgaagaact gaattttacg ctccggacgt
     4561 ttcggatctt ccgacatgcg cagcacgtcg cgcagatgtt cgttatccgc caccgccaga
     4621 cgcagcgcac ctaacgcctt atccgacatg gaacgcaaat catcagcgga gagataagcc
     4681 agctcacgac ggtgtaagcg gcgctcaacg ccgttatctt tcaccatgcg catcaccgca
     4741 caccagaccg ctttggcggt cactacctgc tcgcgcatct caaagtaatc ccgctccagc
     4801 ttgcgcagtt tgcgggtcag gttgtccatc tccgcttcgc agaaggtaag cgctttttcc
     4861 agttgattgc ggcgtgaacg gttattgctc agttgcgcat gcagctcgtc acggcgaata
     4921 cgcgcccgct cttctgcccc gctatcagca cgcacgccga tatcctgcaa ttcacgttgc
     4981 agatcgttga gtagctcttt tttggtgtcg taagaacttt tcagcgaagc cagcacctgg
     5041 ttgtactgac tcaactgcgc tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc
     5101 gcttccgcct gttccagacg ttcacgcagt ttttcgttga gatcgctgtt accgctaagc
     5161 atttctgccg agtcagaata gctaaagtgc gcacgacgct gcaccacttc cgtcagggca
     5221 aacgcctgct gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc ttcttttaac
     5281 tgttcgaact gttccgggtc gctctgcaat accgaaacaa tcggttccag tttcgccagt
     5341 tgattgccaa actgctgaac aaaacgcgcg gcttcctggg cttcatccag
//
LOCUS       DACAPB010000246         5384 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.247, whole genome shotgun sequence.
ACCESSION   DACAPB010000246 DACAPB010000000
VERSION     DACAPB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.247"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..2344)
                     /locus_tag="IT904_004325"
     CDS             complement(<1..2344)
                     /locus_tag="IT904_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP3194015.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAALPQDEKLITGQLDNGLR
                     YMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFE
                     SMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAASFEKLEVDAER
                     GVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRW
                     YQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKE
                     NRVNGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTAR
                     SVKIAPDYQSLFFRVNARGDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLK
                     NAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLR
                     KNQDAFWEQMVNNELAAKKALSPAAILALEKEYTNKKLAAYIFPGRNLSLTVDADPQA
                     EISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIAL
                     ANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQ
                     RITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQ
                     FTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLT
                     RATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALD"
     gene            complement(2389..4761)
                     /locus_tag="IT904_004326"
     CDS             complement(2389..4761)
                     /locus_tag="IT904_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP3194016.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDYVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQTYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKANAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLQQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(4799..>5384)
                     /locus_tag="IT904_004327"
     CDS             complement(4799..>5384)
                     /locus_tag="IT904_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP3194017.1"
                     /translation="QVADASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLK
                     TLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICKALPLPVDDKSLSEILHQVG
                     LGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLR
                     LVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1345 a         1264 c         1228 g         1547 t
ORIGIN      
        1 catcgagcgc catacgcgtc gccagattaa caggtgtccg ggaatcataa cgcttccact
       61 gtgaaacctg tgccacaggt tcattttgtt cttttacagt aaccgatgcg ttgtccgtcg
      121 cgcgagttaa tggtttacct gcggctaatg gcgaatcaga gtgtttgatt gatcctaagt
      181 aacgcgtaat taacgccacg agtttgtctt ctgagacatt accgacaatg acaaacgtga
      241 tatccgctgg agatgaaaac aactggcgat cggcagccag cgcatctgcg gcagtaaact
      301 gtgcaatctg attttcttgc agtaatttcg tgcggtcatc agcatagcgc gtctcataca
      361 tctgctgggc gaatttctcc gctggacgct ggtcgagcgt tttcaacgcc tgaatttgag
      421 cattttgtag cgatgcccaa atattgtcgt taatcgtgct gtgggtgatt cgctggttaa
      481 tcaactggaa accaggttca gggttattag tccgcgcgct aacggagagc aacgtattca
      541 tgccactgac tttactgctc atggttaccg agttttccgc actccagcgt ttcaggctgg
      601 aagaggagag ttcgccaacg ccgcttccgc taactgcttt atttgccagc gcgataagtg
      661 atttttgctg cgcagggaaa cttaaatcgc ctttattcga tacggcaata atttgtagct
      721 tttgctcttc acccgcggat tttgccagaa taacccttgc accattggaa agtgttaatg
      781 atgtcagatt ctcagccagc gtttctttgc tgctaatttc cgcctgtggg tcagcgtcta
      841 ctgttaacga taaatttctg cctgggaaga tgtaagccgc cagctttttg ttggtgtact
      901 ctttttccag cgccaggata gccgcaggag acaatgcttt tttggcggca agctcattgt
      961 ttaccatttg ctcccaaaat gcgtcctggt tctttcttaa ctgctgccat ttttccgcca
     1021 gactttgcac ggtaatttgc tgccacagac gtttcgaaag ttgatatgtc tcttccggcg
     1081 acaagaacgg cgtattattt aatgagctgg atgccaggcg actggtcagc atacgtaaat
     1141 cacgttcagc ttgctgatca accgcatttt tcagccaggt gaggcgggta gatttgacat
     1201 catcgagttc ttcagcagaa aaaccatgct gatcaatggt tgccaactct gccattaatg
     1261 cattcgcagc atcctgcata ttatcgcctc gtgcatttac acggaaaaac agcgactgat
     1321 aatcgggtgc aattttaacg ctgcgtgcag taccgccaga aatagtcttc aactcgcccg
     1381 attgtatgcg ttcctgcaga cgttgattga acagctgaac taacatgctc cattcagctt
     1441 gttcgataaa gctttgctca tcgctcactt gcaccattgg caggcgataa tagagtgcga
     1501 tgccgttcac ccggttttct ttatcattaa tgatattaaa gcgcaggtga ttttcggctt
     1561 ttgtcggcca gacgcgattt tcagctgctt tgttagccgg aagcttactt aaattatcct
     1621 ttatcagcgc cagcgcttct ttactgtcga tatcgccaac cacgataaag gtcatattat
     1681 ttggttgata ccagcgttga taaaattggc gcaattgtgc cggtgtgacc gtggcgacag
     1741 tatccatcag gccgataggt tcacggtcta aattacgggt attagccagc aggaaagggc
     1801 ggcgcgcctg agaggtgcgc catttcgcat cctgatgggc acgccattcc tcagtaatta
     1861 cgccgcgttc agcgtctact tcgagttttt caaagcttgc ggcattactc cattcactga
     1921 agattgccat cacttgttgc agattttgtt tctgcgtagt cggcaaactc acctgataca
     1981 ccgtttcgtc atagctggta taggcattaa catcgcgacc aaaacgcagg cccattgact
     2041 caaatgtttc gatgacttta ttacccggcc atgtttttgt gccgttaaac atcatatgtt
     2101 ctacaaaatg agccacgccg cgctcattgt cttcttcctg caatgaaccg gtatgaattt
     2161 gcagccataa atttacctga tcctttggat gagcatgcgg ataaatcata tatcgcaagc
     2221 cattgtccag ttgcccggta attaactttt catcctgcgg taaggcggca atcagccgcc
     2281 ctgggagcaa cagagttgcc actaatgtca gtaagaaaca gaggtttctc ataattatct
     2341 ccatgcgaaa accgggcgaa tttacccggt taagtaaaat ccgaactatt aaaatttcat
     2401 gctgacatca agccagaaag tacggccact ggcgtaggtc gccacaccgg tattcgttgt
     2461 atcaacagcg gttttgctat ccagtacatt gagaatatcg gcactgatcg tcaggttttg
     2521 ttgttgcaga aattgtggcg tccaggacaa gcgggtgtcc caggtcaggc tgctatccag
     2581 tttttcgtca acatactgct tgtaatctga atattcgctg atgtattgcg cattcgcctt
     2641 acccaggata atgcgagctt tacgcgcttc ttgccaggcc agcgtattcg cccacactaa
     2701 cccgctcggt tgatgcgtga aatccatgtt taaggagatc tttaatgggt tattaaaatc
     2761 tgccactgga acgctatcgt aagagaccag attaccgtta taaaccacct ggttatcacc
     2821 ggtattgctc tcctcataac cattgtttaa cgacaagttg cccttgctct tgatatagct
     2881 aaagacaatt tgtgggttaa tatctacctg gcggatatgc aggggttcgg ccagctcaaa
     2941 actgaggttg aacgaatgcg ttttggtttt gccatcgttg ttatattcag taatggtggt
     3001 tttagtcgcg ctgtcggtac gactgctttt gctgatttga tcatgcgctt cacggtaaac
     3061 atagtttgcg cgcgcaataa cgttcttacc gattttttgc tgcaatccca ttgccagttc
     3121 atcgttataa ggcgttttca aatcctgata acgcgtcagg gttttattgc ctgataccga
     3181 ttccgtccag ctattgcgga tatcacgtaa tcccatatca agaatattcc caccatagta
     3241 acggttataa cctgcggtaa tcattgaggt ttgatcagca aaaatatccc attccgtcat
     3301 aaaacgcggg gagatattgt ggtttgacag atagttgtca tagtcatacc gcacgccggg
     3361 cattaatgac acattacgcc agctaatgcg atccgccata tacagtgtgt agttgtcaat
     3421 tcccaggctg cctttacctt tatggtaaat ggtatggtta gttttctttc cggcagcatt
     3481 aatcacatag gattcagact ggttatggcg ttcagtccat gcatcggaat agatgtattc
     3541 cgcgccgaag taggtttgat gcgaaacatt acccacggcg aatttttgcc agtccaggcg
     3601 tgttttgaag gtgtaattat ctacagcctg ggaaatgtgt cctaatccgc cacgggtgca
     3661 acgccctgta atatcaccat atgtacatga aagttcggtg taccagatgt catgatcgtg
     3721 acgggtataa tcactaatat gatcccaacc aacggtggta cgtagtttgg cccatgcgag
     3781 ctgcgtatcc atatcccatg ccagaccata ggatttatta cccatttcgc gatcagactg
     3841 cgggaaggtg ctggtattat aatcacggct ggagccggta tattttaagg ttaaatcgtg
     3901 ggtaaagcgg tcgctggcaa accaggtaaa tttgctcaat gcagtatcga taacgttttt
     3961 atactgtgcc cgaccggcga caatgccgtc attcgaaaca taatccgcac gggtgatatc
     4021 agactggcgg cgcgataaac cggcggtaac gccaaagtta tcagcgagtt cctgattaaa
     4081 cgacaaggta taaaagttct ttttaaaatc tggagagtaa taagtacttc ctgaagaacc
     4141 ttgattaaat gcgctcttgt tattctcatc gatatgcgat gttaaccagt ccgaacgcgt
     4201 agtgcgataa cccagtttca cgctgctatc atcagcgttg aagcgtttga tctttgcatc
     4261 aattaccccg ccattgaaac gaccgaattc aaccggcaca aaactgtcat aaagcgtcac
     4321 atagtccagt aagctgacat caagataata cccctgtgac atcccgctaa tattggttgc
     4381 actactggca tcggactcat tcgctgggtt caggttatta gtggcactaa taccatcaat
     4441 caaataggca ttctggtagg gcgacgcacc gtgaatagag attttctccg ggcgaatatc
     4501 tccctggttc aacgaggtac tttgcgttga atccatgcgt accgcagggt tggttctcag
     4561 cagatcgctg atattgccat tcccggtcgg taaacgctcg atgctttcac tggtgtaatg
     4621 cgtgttgccg tttacgggta cagggacagg agcatagacc gtcatctctt caaaaaaatg
     4681 catgtacatg tttttgtcat cgacggcctg cgccacatca gcgccacata aaatgacgcc
     4741 aggaataaga actcgcttca tttatttata tctccgtaca gcagggcttt tattgttttt
     4801 ataaaaccgc gctaatgtca caaatatcat cggccaggtt ccagacaccg ggttgatgtg
     4861 taaccataat gacgccgctt gtgggtagtt tttcacgcac taaacgcagt aagcggatag
     4921 cctcttgttc ctcaagatga gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta
     4981 aaattaatcg tgccagggcg atacgttgtt tttcgccgct ggaaagaata tctccccagc
     5041 gatcgtggtc atgaatacgc gcagccaatt tccccagacc aacctgatgc agtatttcgc
     5101 tcaacgattt atcgtctacg ggcagaggaa gtgctttaca aataatctct ttcagtaagc
     5161 cggttttaat taacggtgtt tgtgacacat accaactgtc agcaggagaa gaaatatcac
     5221 ctttaaacca cggccagcag tgggataatg ttttaagcag tgtggttttt cccgcaccag
     5281 agtaaccttt cagtaatagc catttgcctg gcgaaacatg aaagtttagg ttctctaata
     5341 tgatcttatt atcaggcgta cgaatactcg catcagccac ttgt
//
LOCUS       DACAPB010000247         5368 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.249, whole genome shotgun sequence.
ACCESSION   DACAPB010000247 DACAPB010000000
VERSION     DACAPB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.249"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1630)
                     /locus_tag="IT904_004328"
     CDS             complement(<1..1630)
                     /locus_tag="IT904_004328"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAP3194018.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAK"
     gene            complement(1651..2277)
                     /locus_tag="IT904_004329"
     CDS             complement(1651..2277)
                     /locus_tag="IT904_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAP3194019.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(2732..2941)
                     /gene="fumD"
                     /locus_tag="IT904_004330"
     CDS             complement(2732..2941)
                     /gene="fumD"
                     /locus_tag="IT904_004330"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAP3194020.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            3498..4910
                     /gene="pykF"
                     /locus_tag="IT904_004331"
     CDS             3498..4910
                     /gene="pykF"
                     /locus_tag="IT904_004331"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAP3194021.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAKKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            5221..>5368
                     /locus_tag="IT904_004332"
     CDS             5221..>5368
                     /locus_tag="IT904_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931354.1"
                     /note="major outer membrane lipoprotein; interacts with
                     peptidoglycan to maintain the integrity of the cell
                     envelope; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein"
                     /protein_id="HAP3194022.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVN"
BASE COUNT         1474 a         1312 c         1122 g         1460 t
ORIGIN      
        1 acttagcata tttaattttt gcgtcattga ttggcgtgta gtttttggtt tcatcgtagg
       61 catcttcaga accaaaaagc tctttcgcca cttcacgttg cagtttcaat ggcaagccgg
      121 aaccaataaa gttgatatgg gtatgtgtca tgcagtcacg gttgaacata cagttaacaa
      181 tggaaccgac ctgcgctgac gcttcattgg catggtgaac cggatagcca aacggcgacc
      241 agagtttatt tttcgcgtag ccccagtact cttcacccaa attccagcgt tctgcgatgg
      301 catatgaacc atcagccagg tgactcagct cacccacacg atgcgccaga cggtagtaaa
      361 aatctttaat gaaattaacg tcacccgctt ccagttgatc ccagtgaatt tccgcatact
      421 cttcagcagg cagaacacgc ttgaacaccc ctttgctgta acaataagta aagtcgcgat
      481 gcaactgccc gtagttacac cataggccgt agtcatcgaa caaattcaga ccaaccaagt
      541 taccaatcac acggccatcg tctttatctt caaaatcttt cggcccgttc gggaagatgg
      601 tggtatggac aaagtttgct acacaagtgt taccacctgt gctgggcacg ccaaactctt
      661 tcacccgagg aatattcatt tgggtcatac aacggatcgg gcaagagtgg cagccgctca
      721 tttttactgt gtatttctct gccgccggtc ctaagtcaaa aacggattta taggtacgaa
      781 agccgaccgt attctgattg cctggcggaa tttcacccgt ttcaatcgga ccaccttcag
      841 ccgcgcccca aaacagccct ttacgtgctg tccagcgtga cttgggatct gaatactctg
      901 cccacgattg tggcgtactt ggcacgacat ggttgttatt cgcaccaata agttcagtca
      961 tcatgtaatc attgagacgc ttcatctcct gacgatcggc aatgttgacc cctttcgtcc
     1021 cttcaaccgc aatcgctttc aggtttttcg aacccattat tgcgccagtt cccgcaccgc
     1081 cgctgtggtt acggctattc aacatgccag agagaggaac aaggttttcc ccagcctgac
     1141 caatagccgc cacacaggtt tccggactgg tcaatcgaca aatttcttcc gtcgttgcgc
     1201 gcgtcccttt tccccataag aaatcggctt tttccaggct aactttgtca tctttaatct
     1261 tcagccatac cggtgatttc gccttccctt cgataataat gacgtcgtat ccagcgaatt
     1321 tcatttgcgc tgcaaaaaag ccacccatat gggcatcgac gactaaattt cctttggtaa
     1381 aagtagaaag tgaggtgata tttacgcgag aactacaggg ggcaccagat ccagttaatg
     1441 ggccggtagc aaagactaat ttattcgctt catcgaaagg tttcgtgcct ggcggtactt
     1501 cgtcatacat aattttgtag ccgaagccca tgccaccgac aaaactttta aacttactgg
     1561 aatcttcgag ggtaatattt cctgtcgtga gattgactct taatatatta cctgtccaac
     1621 cgttagccat gattttttcc tttgcaagat tcacacagta atatctttcc actcgataat
     1681 ttttaacgcc cctgttgggc aggcgtttgc gcattcaccg cataacacac atttcgagga
     1741 ttttttactt tcggtattta cggtggccat catccacggg catgccgtag tacaggcgct
     1801 acagccaata cagcgtttat gatcgacggt aatacagcct tctttctgct gccaggtaat
     1861 cgcaccaatc gggcagacgt tcatgcattg cggctctttg cattgacggc aggtgtccgc
     1921 ggtatagttg agatcgccat acaggccgcc gccagagcca accccgttgt cgccaaagaa
     1981 ataattgcga tggattttga tacgggagaa gaatgtccct actgagccat cgttgaagtt
     2041 ggtacaagag atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac
     2101 ccctttgggg gtatttatca accctactgt gccgctatca atatcttcct gtttgcaacc
     2161 caaaagtgac agtaacgcag gggcaattgt tagaccagca aggccttttc ctgatatacg
     2221 caagaattct aatcgcgtta agccaatatc taatagtgga cgatcaaccg ggttcattta
     2281 tttcgcatcc tcttcgcaaa cacagtgctg acaattcagc agcctgatat taagcattca
     2341 actaatacgc tgctgactct gtccccaaca ccagatattt tccctaaccc gatagtgtat
     2401 gagaaaagat taacgccgat aaactaatcc tttatggtta ggagaaaaaa taatgtgatt
     2461 attctgagcc cttaacattg atcgttatca attaaaatta caaagcaaga aattataagt
     2521 gaactgatat ttattatcat ttgaaataaa tttaacttaa gcgacaatta tttgtgacgt
     2581 tcattgcaga aaataaaaat aacattatcg ctataaatta aaatacatac cgaaattcgt
     2641 ataatttcag cagttgttat ttagaatgat taactggacc gcaactgaag caagaatatc
     2701 aggatgaagc ttaccgcctc atcctgaatg gtcattttac cagtgcgtta atgacggttt
     2761 ccatcgcctg cttttccagc tcactgcgct gaatgcgttt attggctaat gctgtacgta
     2821 atacgcctgc gataacaata tgctttggtt cctgccccag atcgcgcatt tccagcacaa
     2881 ctttaccgac aactctgcac atttctcggt acagttcatc ctcttttgtt cgatttccca
     2941 ttctttatac ctatttatca tgccaactat cagcatatgt caatctaacc aattaaacaa
     3001 aaatcaaaca aaatcagaca aataacgcga taaattattt taactgtagc aattgagcga
     3061 tgatatattt atacaccgga tgaactttca cttatcctca cactgacaac ttcggcacca
     3121 gacgttgcgc aaacagtgaa gtttttgcgt aaccttttcc ctggaacgtt aaatctttga
     3181 taacaattta ttgtctaaca agttgtatat tttttgaaac gctgtttttg ttttcctttt
     3241 ggattaattt cagcgtataa tgcgcgccaa ttgactcttg aatggtttca gcactttgga
     3301 ctgtagaact caacgactca aaaacaggca ctcacgttgg gctgagacac aagcacacat
     3361 tcctctgcac gctttttcga tgtcacctat ccttagagcg aggcaccacc actttcgtaa
     3421 taccggattc gctttccggc agtgcgccca gaaagcaagt ttctcccatc cttctcaact
     3481 taaagactaa gactgtcatg aaaaagacca aaattgtttg caccatcgga ccgaaaaccg
     3541 aatctgaaga gatgttagct aaaatgctgg acgctggcat gaacgttatg cgtctgaact
     3601 tctctcatgg tgactatgca gaacacggtc agcgcattca gaatctgcgc aacgtgatga
     3661 gcaaaactgg taaaaccgcc gctatcctgc ttgataccaa aggtccggaa atccgcacca
     3721 tgaaactgga aggcggtaac gacgtttctc tgaaagctgg tcagaccttt actttcacca
     3781 ctgataaatc tgttatcggc aacagcgaaa tggttgcggt aacgtatgaa ggtttcacta
     3841 ctgacctgtc tgttggcaac accgtactgg ttgacgatgg tctgatcggt atggaagtta
     3901 ccgccattga aggtaacaaa gttatctgta aagtgctgaa caacggtgac ctgggcgaaa
     3961 acaaaggtgt gaacctgcct ggcgtttcca ttgctctgcc agcactggct aaaaaagaca
     4021 aacaggacct gatctttggt tgcgaacaag gcgtagactt tgttgctgct tcctttattc
     4081 gtaagcgttc tgacgttatc gaaatccgtg agcacctgaa agcgcacggc ggcgaaaaca
     4141 tccacatcat ctccaaaatc gaaaaccagg aaggcctcaa caacttcgac gaaatcctcg
     4201 aagcctctga cggcatcatg gttgcgcgtg gcgacctggg tgtagaaatc ccggtagaag
     4261 aagttatctt cgcccagaag atgatgatcg aaaaatgtat ccgtgcacgt aaagtcgtta
     4321 tcactgcgac ccagatgctg gattccatga tcaaaaaccc acgcccgact cgcgcagaag
     4381 ccggtgacgt tgcaaacgcc atcctcgacg gtactgacgc agtgatgctg tctggtgaat
     4441 ccgcaaaagg taaatacccg ctggaagcgg tttctatcat ggcgaccatc tgcgaacgta
     4501 ccgaccgcgt gatgaacagc cgtctcgagt tcaacaatga caaccgtaaa ctgcgcatta
     4561 ccgaagcggt atgccgtggt gccgttgaaa ctgctgaaaa actggatgct ccgctgatcg
     4621 tggttgctac tcagggcggt aaatctgctc gcgcagtacg taaatacttc ccggatgcca
     4681 ccatcctggc actgaccacc aacgaaaaaa cggctcatca gttggtactg agcaaaggcg
     4741 ttgtgccgca gcttgttaaa gagatcactt ctactgatga tttctaccgt ctgggtaaag
     4801 aactggctct gcagagcggt ctggcacaca aaggtgacgt tgtagttatg gtttctggtg
     4861 cactggtacc gagcggcact actaacaccg catctgttca cgtcctgtaa tattgctttt
     4921 gtgaattaat ttgtatatcg aagcgccctg atgggcgctt tttttattta atcgataacc
     4981 agaagcaata aaaaatcaaa tcggatttca ctatataatc tcactttatc taagatgaat
     5041 ccgatggaag catcctgttt tctctcaatt tttttatcta aaacccagcg ttcgatgctt
     5101 ctttgagcga acgatcaaaa ataagtgcct tcccatcaaa aaaatattct caacataaaa
     5161 aactttgtgt aatacttgta acgctacatg gagattaact caatctagag ggtattaata
     5221 atgaaagcta ctaaactggt actgggcgcg gtaatcctgg gttctactct gctggcaggt
     5281 tgctccagca acgctaaaat cgatcagctg tcttctgacg ttcagactct gaacgctaaa
     5341 gttgaccagc tgagcaacga cgtgaacg
//
LOCUS       DACAPB010000248         5353 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.250, whole genome shotgun sequence.
ACCESSION   DACAPB010000248 DACAPB010000000
VERSION     DACAPB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.250"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..599)
                     /locus_tag="IT904_004333"
     CDS             complement(<1..599)
                     /locus_tag="IT904_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP3194023.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEG"
     gene            complement(671..1426)
                     /gene="gloB"
                     /locus_tag="IT904_004334"
     CDS             complement(671..1426)
                     /gene="gloB"
                     /locus_tag="IT904_004334"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAP3194024.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSVLPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            1460..2182
                     /locus_tag="IT904_004335"
     CDS             1460..2182
                     /locus_tag="IT904_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP3194025.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKSKPRIRQAVGATRQCRKPQA"
     gene            complement(2179..2646)
                     /gene="rnhA"
                     /locus_tag="IT904_004336"
     CDS             complement(2179..2646)
                     /gene="rnhA"
                     /locus_tag="IT904_004336"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAP3194026.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            2711..3442
                     /gene="dnaQ"
                     /locus_tag="IT904_004337"
     CDS             2711..3442
                     /gene="dnaQ"
                     /locus_tag="IT904_004337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP3194027.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            3575..3651
                     /locus_tag="IT904_004338"
     tRNA            3575..3651
                     /locus_tag="IT904_004338"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3609..3611,aa:Asp,seq:gtc)
     gene            3981..4766
                     /locus_tag="IT904_004339"
     CDS             3981..4766
                     /locus_tag="IT904_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194028.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNDKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(4903..5346)
                     /locus_tag="IT904_004340"
     CDS             complement(4903..5346)
                     /locus_tag="IT904_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194029.1"
                     /translation="MFFMAILFFPAFNASLFFTGVKPLYSIIKCSTEIFYDWRMLILC
                     FGFMSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLLIAIVIAPLIAPF
                     VTGYVNTNYHPCGNNTGIFPGAIYIKNGMKCNNGYISRKEDSAVK"
BASE COUNT         1481 a         1165 c         1200 g         1507 t
ORIGIN      
        1 ccttcgccgc tgttataagc cgctacggtc agaagccagt cgccgtcaaa cattttgttc
       61 agacgctgca tcatattcag cgcggcagtt gttgaagcaa caacatcgcg acgcgcgtca
      121 taattgcggg tctgtttcaa accataattg cgccccgtgc tcggaatgat ctgccagatg
      181 cctgcggcat tggcgccaga cgttgcgtga ggatcaaaag cgctctccac tatgggtagt
      241 agtaccagtt ccataggcat gttacgtttt ttaacttgcc ctgctatcca gtacatatac
      301 ggctctgccc gtaaagttac atcgtggaga tagctcttat tgcgtaaata tttctgtttc
      361 tgttcgcgaa tccggtcatt ttccggaatt cccatcttta gctcgtcgcc aatgaaagcc
      421 cacaagtcac catctggcgc gatagacgtc ccatcgtcca tccatcgtgc ctgacttgta
      481 aactttgctg cttccccttg accagctgca gaaaggctct gtgcgtgctg ttgaacgttg
      541 ccggtactct ggcaacccac gagcaggaca gaggcgagta atatcgcttt tgccttcatg
      601 tgtgtgtcaa tagttgctta aaagacgacc gatcataacg gcgaacggag ccgatgacaa
      661 gaaagtttta tcagaaccta tctttctttg accttaacca tgcaaaacgc tcttcaggtt
      721 gttgcaataa tgtttcttca ttaattacat taattaaatc aatatcttcc gttcttaaaa
      781 aaacattaat ttgccgctca tttttcagaa ttacgggtag tgttatttga ttttttgccc
      841 gtaactcctt aactttacga taataatcat ttatggacaa atcgtgcgga agaatactca
      901 aagcaaactt catatttgat aaggtatatt catgagcaca acataccaat gtatcgtcag
      961 gtaacacact taacttttta agtgattgat acatttgtga tgctgtccct tcaaacaacc
     1021 gaccacaccc accagaaaac agtgtgtcgc cgcaaaatag ataaggttta ctgaagtaac
     1081 agatatgtcc taaagtgtga cccggcgtag caattacact aaattcatgc cccaaaacga
     1141 aggcagtttc gccatctttg actacctgtg ttgttccctt atcttgtgtc tcttgtggac
     1201 catacaccac aatttgtgga aacttttcca ccagttcttt tacgccgcca acgtgatcgt
     1261 ggtgatggtg ggtgagaaat atggcctccg gttgccagtt attggcggca atggcgttta
     1321 atactggctc tgcgtctccg ggatcgacaa tcaggcagcg acctgcttca tcattcaaaa
     1381 cccagatgta attgtcatca aaggcgggaa tactgttaag attcatagat tacctcttag
     1441 tgtgaaacgg aaggttgtga tgaaaccggc aagagtccct caaactgtcg tggctcctga
     1501 tcgctggggc gatttgccct ggggagagct ttatcgcaag gcgctggagc gccagctcaa
     1561 cccgtggttc actaaaatgt atggttttca tctgcttaag attggcaatt taagcgcaga
     1621 aatcaattgc gaagcgtgcg cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc
     1681 cgtccaggtg caggcggatc cacttcatct tccttttgcc gataaatccg ttgatgtttg
     1741 tctactggca catacattgc cgtggtgcac cgatccgcat cgtttattgc gtgaaaccga
     1801 tcgggtattg attgatgatg gctggctggt gattagtggc ttcaatccca ttagtttaat
     1861 gggattacgc aaacttgtgc cggtattgcg aaaaacctcg ccctataaca gccggatgtt
     1921 tactctgatg cggcaactgg actggctctc tttgttgaat tttgaagtgc tgcacgccag
     1981 ccgtttccac gttctcccgt ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc
     2041 tgcgcttggt tgcttacaac ttattgttgc ccggaaacgg actattcctt taacgctaaa
     2101 cccgatgaaa cagagtaaaa gcaagccacg aattcgccag gcggttggag ccacccggca
     2161 atgtcgtaaa ccacaagctt aaacttcgac ttggtagccc gtatcttcca gtgtgggatt
     2221 catcgccgcg gcacgagcca gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc
     2281 tttaacccat tcccatttga tttgatgttg ccctagggca gcatcaagac gttgccagag
     2341 atcgacattt tttactggtt ttttgtctgc ggttttccag ccacgttttt tccagttatg
     2401 gatccactgg gtgatacctt ggcggacata ctggctgtcg gtactcaaaa tgacttcgca
     2461 atgttctttt aacgcctcca gcgcgacaat agcggccatc aactccatac ggttgttggt
     2521 ggtgcgggtg tagcctgcgc taaaggtttt ctcgcgtccg cgatagcgta aaatagcgcc
     2581 gtaaccccca ggtcctggat tgcccagaca cgaaccatcg gtgaaaattt ctacctgttt
     2641 aagcatctct ggtagacttc ctgtaattga atcgaactgt aaaacgataa gtctgacata
     2701 aatgaccgct atgagcactg caattacacg ccagatcgtt ctcgataccg aaaccaccgg
     2761 tatgaaccag attggtgcgc actatgaagg ccacaagatc attgagattg gtgccgttga
     2821 agtggtgaac cgtcgcctga cgggcaataa cttccatgtt tatctcaaac ccgatcggct
     2881 ggtggatccg gaagcctttg gcgtacatgg tattgccgat gaatttttgc tcgataagcc
     2941 cacgtttgcc gaagtagccg atgagttcat ggactatatc cgcggcgcgg agttggtgat
     3001 ccataacgca gcgttcgata tcggctttat ggactacgag ttttcgttgc ttaagcgcga
     3061 tattccgaag accaatacct tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat
     3121 gtttcctggt aagcgcaaca gcctcgatgc gttatgtgcc cgctacgaaa tagataacag
     3181 taaacgaacg ctacacgggg cattactcga tgcccagatc cttgcggaag tttatctggc
     3241 gatgaccggt ggtcaaacgt cgatggcttt tgcgatggaa ggagagacac aacagcaaca
     3301 aggtgaagca acaattcagc gcattgtacg tcaggcaagt aagttacgcg ttgtttttgc
     3361 gacagatgac gagcttgcag cacatgaagc ccgtcttgat ctggtgcaga agaagggcgg
     3421 aagctgcctc tggcgggcat aaatacctgt aaaaggcgct aaaaatagcg acttgggcga
     3481 tttttgcagc aaacgattca aaagatgaga aaaaccgttg acgaaggtcg aggcaatccg
     3541 taatattcgc ctcgttccca acggaacaca acgcggagcg gtagttcagt cggttagaat
     3601 acctgcctgt cacgcagggg gtcgcgggtt cgagtcccgt ccgttccgcc aaattcactc
     3661 atgaaaatga gttcagagaa ccgcaagatt tttaattttg cggttttttt gcatttaaat
     3721 cccgctattt cttcggtcta tggttttgtt atgctgtaaa tgtgttaatc acatatggta
     3781 gaccatatca ttaatattta atcatactat cgaatcattc aatttaaata tatttgaatg
     3841 ttatgatttt catttttcta ttgatttaat gaatttatat atactgttat ttttgtttgt
     3901 tcttattcaa gtggagtata taattaaaaa tcgttagcta aacattacca tgattatgac
     3961 aggcgaggga gtgtccgatt atgaatttaa gaagggtttg ttgtatatgt gtattattct
     4021 cgctgcttgc aggatgtgcc tctgaatctt ctattgatga aaagaagaaa aaagctcaag
     4081 tcacacaaag taatattaat aaaaacactc cccagcaact gacagacaaa gatttattcg
     4141 gtaatgaaac cactctggcc gtatccgaag aggatattca agctgcgctt gatggagatg
     4201 agttccgcgt tccccttaat tctcctgtaa tacttgttca atccggcaac cgcgcaccgg
     4261 aaaccattat gcaggaggag atgcgaaaat attatactgt ttccacattc tctggtatcc
     4321 cggacaggca aaagcctctg acttgtaaca aaaacaaaga taaaaatgag aacgaggatg
     4381 ttgccagtgc tgagaatatg aactggatgc aggcactgcg ttttgtggct gcaaaaggac
     4441 atcagaaagc gattattgtt taccaggata tgttgcagac aggaaaatat gactctgcgc
     4501 tgaaatcaac agtatggtcc gactataaaa atgacaaact cactgacgct atctccctgc
     4561 gctacctggt acgtttcacg ctggtggatg tggcaacagg tgagtgggct acctggtcgc
     4621 cggtgaatta tgaatataaa gtgcttccac cattgcccga caagaatgaa gccagtacga
     4681 ctgatatgac agagcagcaa atcatgcaac ttaagcaaaa gacctataaa gcgatggtaa
     4741 aagatttggt taaccgctat caataataaa ttatatctgc cgccgggaat ttctggcggc
     4801 aataatacaa aattctttgc ataagaaatt tttcttgaca aagacagtgc aagaaaggac
     4861 atggaatccg gagttaaaaa ctgtttgttt tagaatattt aattatttaa ctgctgaatc
     4921 ttccttgcga gaaatatatc cattattaca tttcatgcca tttttaatat agattgctcc
     4981 tgggaaaatt cccgtattat taccgcaggg gtgataatta gtattgacat atcctgtgac
     5041 aaaaggagct attaaaggtg ctattacgat agctattagt aaaaatataa gagttagctg
     5101 tattgttatg tctgtggcga aattgactac cttcgttttt ttgattaaga atgattttat
     5161 tatcgtaagt aaaattacat gaatatttaa aaaggaaaac gacatgaaac cgaagcacag
     5221 aatcaacatt ctccaatcat aaaatatttc cgtggagcat tttattattg aatatagagg
     5281 tttaactccg gtaaaaaaca aagaagcatt gaatgcaggg aaaaataata tggccataaa
     5341 aaacatcgaa aga
//
LOCUS       DACAPB010000249         5289 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.251, whole genome shotgun sequence.
ACCESSION   DACAPB010000249 DACAPB010000000
VERSION     DACAPB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.251"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(465..728)
                     /gene="rpsT"
                     /locus_tag="IT904_004341"
     CDS             complement(465..728)
                     /gene="rpsT"
                     /locus_tag="IT904_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP3194030.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            831..1049
                     /locus_tag="IT904_004342"
     CDS             831..1049
                     /locus_tag="IT904_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAP3194031.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            1057..1998
                     /gene="ribF"
                     /locus_tag="IT904_004343"
     CDS             1057..1998
                     /gene="ribF"
                     /locus_tag="IT904_004343"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAP3194032.1"
                     /translation="MKLIRGIHNLSQAPQEGYVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKLLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            2041..4857
                     /gene="ileS"
                     /locus_tag="IT904_004344"
     CDS             2041..4857
                     /gene="ileS"
                     /locus_tag="IT904_004344"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP3194033.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4857..>5289
                     /gene="lspA"
                     /locus_tag="IT904_004345"
     CDS             4857..>5289
                     /gene="lspA"
                     /locus_tag="IT904_004345"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAP3194034.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAI"
BASE COUNT         1233 a         1298 c         1474 g         1284 t
ORIGIN      
        1 atgaattttt attaatttaa tttatgagtg aatgtgacta ctatcaaaaa tcgctcatta
       61 attttaagaa gtacaatata aataggtatt taacctgtgt tgattgctgt taataattta
      121 caaggtctaa tgaggagata tttatgaatg gttaaagtat ataggaattt aattcaaaaa
      181 tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt ctttgtaggg
      241 ggtctggaga ttgagtagat attcttgttc agaatgtatc agccgatggt tctacgattc
      301 ttaagccacg aagagttcag atagtacaac ggcatgtctg caagaacctg caacttttga
      361 ctatctggca accggcagtg tgttctctca cgcatcacaa aagcagcagg cataaaaaaa
      421 cccgcttgcg cgggcttttt cacaaagctt cagcaaattg gcgattaagc cagtttgttg
      481 atctgtgcag tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta
      541 gcagcctgac ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc agcagctttg
      601 tcgccagctt cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg
      661 cttgcgttgt gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata
      721 ttagccaagg tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat
      781 gcccttttag ccttcttttg tcaatggatt tgtgcaaata agcgccgtta atgtgccggc
      841 actcgttacg tagtgatggc gcaggattct accagcttgc ggggtgtgaa tacagctttt
      901 ccgcgataaa aattgcagca ggcggtcagt ttcttcccgt gatttgcgcc atggcaatga
      961 aaagccactt ctttctgatt tcggtactca atcgccggtt aaccttgacc gctgtacaag
     1021 gtatactcgg acgattttca ctgttttgag ccagacatga agctgatacg cggcatacat
     1081 aatctcagcc aggccccgca agaagggtat gtgctgacta ttggtaattt cgacggcgtg
     1141 catcgcggtc atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa gcgcaactta
     1201 ccggtgatgg tgatgctttt tgaacctcaa ccactggaac tgtttgctac cgataaagcc
     1261 ccggcacggc tgacccggct gcgggaaaaa ctgcgttacc ttgcagagtg tggcgttgat
     1321 tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt taaccgcgca aaatttcatc
     1381 agcgatcttc tggtgaagct tttgcgcgta aaatttcttg ccgtaggtga tgatttccgc
     1441 tttggcgctg gtcgtgaagg cgatttcttg ttattacaga aagctggcat ggaatacggc
     1501 ttcgatatca ccagtacgca aactttttgc gaaggtggcg tgcgtatcag cagcaccgct
     1561 gtgcgtcagg cgctggcgga tgacaatctg gctctggcag aaagtttact ggggcacccg
     1621 tttgccatct ccgggcgtgt agtccacggt gatgaattag ggcgcactat aggtttcccg
     1681 acggcgaatg taccgctacg ccgtcaggtt tccccggtga aaggggttta tgcggtagaa
     1741 gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa acatcggaac acgcccaacg
     1801 gttgccggta ttcgccagca gctggaagtg catttgttag atgttgcaat ggacctttac
     1861 ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg atttgcgtcg
     1921 ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga attttttggg
     1981 ctaacaaaac cggcttaagc ctgttatgta atcaaaccga aacacggaac cgagaatctg
     2041 atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat gcgtggcgat
     2101 ctcgccaagc gcgaaccggg aatgctggcg cgttggactg atgatgatct gtacggcatc
     2161 atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc tccttatgcg
     2221 aatggcagca ttcatattgg tcactcggtt aacaagattc tgaaagacat tatcgtgaag
     2281 tccaaagggc tttccggtta tgactcgccg tatgtgcctg gctgggactg ccacggtctg
     2341 ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg gtgagaaatt caccgccgcc
     2401 gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca acgcaaagac
     2461 tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat ggacttcaaa
     2521 actgaagcca acatcatccg cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa
     2581 ggcgcgaagc cggtacactg gtgcgttgac tgccgttctg cgctggcgga agcggaagtt
     2641 gagtattacg acaaaacttc tccgtccatc gacgttgctt ttcaggcggt cgatcaggat
     2701 gcactgaaag caaaatttgc cgtaagcaac gttaacggcc caatctcgct ggtgatctgg
     2761 accactacgc cgtggactct gcctgcgaac cgcgcaatct ctattgcacc tgatttcgac
     2821 tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct ggttgaaagc
     2881 gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa aggtgcggag
     2941 cttgagttgc tgcgctttac ccatccgttt atgggcttcg acgttccggc aatcctcggc
     3001 gatcacgtta ccctggatgc cggtaccggt gccgttcaca ccgcgcctgg ccacggcccg
     3061 gacgactatg tgatcggtca gaaatacggc ctggaaaccg ctaacccggt tggcccggac
     3121 ggcacttatc tgccgggcac ttatccgacg ctggatggcg tgaacgtctt caaagcgaac
     3181 gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga gaaaatgcag
     3241 cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc gacgccgcag
     3301 tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc
     3361 gtgcagtgga tcccggactg gggccaggcg cgtatcgagt cgatggttgc taaccgtcct
     3421 gactggtgta tctcccgtca gcgcacctgg ggtgtaccga tgtcactgtt cgtgcacaaa
     3481 gacacggaag agctgcatcc gcgtaccctt gaactgatgg aagaagtggc aaaacgcgtt
     3541 gaagtcgatg gcatccaggc gtggtgggat ctcgatgcga aagagatcct cggcgacgaa
     3601 gctgatcagt acgtgaaagt gccggacaca ttggatgtat ggtttgactc cggatctacc
     3661 cactcttctg ttgttgacgt gcgtccggaa tttgccggtc acgcagcgga catgtatctg
     3721 gaaggttctg accaacaccg cggctggttc atgtcttccc taatgatctc caccgcgatg
     3781 aagggtaaag cgccgtatcg tcaggtactg acccacggct ttaccgtgga tggtcagggc
     3841 cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc aggatgtgat gaacaaactg
     3901 ggcgcggata ttctgcgtct gtgggtggca tcaaccgact acaccggtga aatggccgtt
     3961 tctgacgaga tcctgaaacg tgctgccgat agctatcgtc gtatccgtaa caccgcgcgc
     4021 ttcctgctgg caaacctgaa cggttttgat ccagcaaaag atatggtgaa accggaagag
     4081 atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga agacatcctc
     4141 aaggcgtacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg cttctgctcc
     4201 gttgagatgg gttccttcta cctcgacatc atcaaagacc gtcagtacac cgccaaagcg
     4261 gacagtgtgg cgcgtcgtag ctgccagact gcgctatatc acatcgcaga agcgctggtg
     4321 cgctggatgg caccaatcct ctccttcacc gctgatgaag tgtggggcta cctgccgggc
     4381 gaacgtgaaa aatacgtctt caccggtgag tggtacgaag gcctgtttgg cctggcagac
     4441 agtgaagcga tgaacgatgc gttctgggac gagctgttga aagtgcgtgg cgaagtgaac
     4501 aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg gctcgctgga agcggcagta
     4561 accttgtatg cagaaccgga actgtcggcg aaactgaccg cgctgggcga tgaattacga
     4621 tttgtcctgt tgacctccgg cgctaccgtt gcagactata acgacgcacc tgctgatgct
     4681 cagcagagcg aagtactcaa agggctgaaa gtcgcgttga gtaaagccga aggtgagaag
     4741 tgcccacgct gctggcacta cacccaggat gtcggcaagg tggcggaaca cgcagaaatc
     4801 tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt tgcctgatga
     4861 gtcaatcgat ctgttcaaca gggctacgct ggctgtggct ggtggtagtc gtgctgatta
     4921 tcgatctggg cagcaaatac ctgatcctcc agaactttgc tctgggggat acggtcccgc
     4981 tgttcccgtc gcttaatctg cattatgcgc gtaactatgg cgcggcgttt agtttccttg
     5041 ccgatagcgg cggctggcag cgttggttct ttgccggtat tgcgattggt attagcgtga
     5101 tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat atcgcttacg
     5161 cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac ggcttcgttg
     5221 tcgatatgat cgacttctac gtcggcgact ggcacttcgc caccttcaac cttgccgata
     5281 ctgccatct
//
LOCUS       DACAPB010000250         5188 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.252, whole genome shotgun sequence.
ACCESSION   DACAPB010000250 DACAPB010000000
VERSION     DACAPB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.252"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1142)
                     /locus_tag="IT904_004346"
     CDS             complement(<1..1142)
                     /locus_tag="IT904_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAP3194035.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENYMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASP"
     gene            complement(1211..2623)
                     /locus_tag="IT904_004347"
     CDS             complement(1211..2623)
                     /locus_tag="IT904_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP3194036.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVYIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKKMTIKAP"
     gene            complement(2642..4519)
                     /gene="bglF"
                     /locus_tag="IT904_004348"
     CDS             complement(2642..4519)
                     /gene="bglF"
                     /locus_tag="IT904_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAP3194037.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(4653..>5188)
                     /gene="bglG"
                     /locus_tag="IT904_004349"
     CDS             complement(4653..>5188)
                     /gene="bglG"
                     /locus_tag="IT904_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAP3194038.1"
                     /translation="CQFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRL
                     GVQLPKDEVGFIAMHLVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQ
                     RLVTHLKFLSWRILEHASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISP
                     AEIMFLAINIERVRKEH"
BASE COUNT         1368 a         1356 c         1122 g         1342 t
ORIGIN      
        1 aatggacttg cgccagcata acggccaaag ttactggcga tagaattatt cgcgaccagg
       61 aaggagaatt cgttgaagcc acctttatca aatttctgcg ttaaagatgt gccaaacatc
      121 caggtatctt tccagtcata atacccgtta ttatcctgat tatctttttc cgatgcgctt
      181 tcgtttgccg tgacataacg accacttacc attaaggtgg ctttatccca taacgggata
      241 tctttatagc gtaaatcaat ggtattggta ttaatctgct gtttgttttg caggctgcga
      301 tcgtaatcat cgacatcttc gcgaaccagc gcgatatcaa ttttacccgg accgactttc
      361 cagttttcca gacctacacc cgctgcggca tcagtacgct gcgttttcca gtcaagcatc
      421 tggatttcaa ttttcggcgc accgtgttta cccacccaga aatcagcctc tggcgcaaag
      481 ggcaggaaac ctttggtggt aacgtacata tcggagaact gcatatagtt ctcgccaccg
      541 gcgttatcgc caaaccagcc ggtagagtac tgctgaccaa cgttaccatc catcatcaca
      601 acggcatcaa cccgtttgcc gttttcgttg tagacacgtt gttttaactg caaatcaaac
      661 cagccggagt attcgttacc aaagcggccc agagaaccaa tcgcccatga tttaggtgaa
      721 ccatgagagg cggtccccca accagaacgg taatagccgt tgtaactaaa gccaatttca
      781 tctttcacaa atttactgaa atctttcaga gtcatggaag aataaatcga gcctgtctca
      841 ctggcatttt tctcttcatt tttgaccagt acagcatcag gttttgcggc actactggtc
      901 gggaacggat tggcggcata cccttgatca ttcgtactta cgctacgatt caccgtcgct
      961 ggcgtatact ttttcttctc ttcatcttta tactttttga gttcgctttg cgtttctctt
     1021 aacgcctttt ctaataactc aaggcgttgt tccaccgtta atgattcagc caatgattgt
     1081 gcagaaaagg ctaacggtgc cattaataag atggcagagg taataagatt tcgtctaaac
     1141 ataatatccc tttatggtgc aaagaaagaa ttaacgcatc gcatccagac tgttctgaat
     1201 gcgacgataa ttaaggtgct ttaatggtca ttttttttaa tgacagcccc cgcgtcttga
     1261 tcacctctgc ataccatccg aagcttttct tgcgtgttct tgtgaggctt ccttcgccat
     1321 tatcatcacg atccacataa ataaagccgt agcgcttaga catttgtgaa tgagatgcac
     1381 tgactaaatc aattggcccc caactggtgt accccataat atacacacca tcggcaatcg
     1441 cttcatttac ctgtaccagg tgatcgttta aataggcaat tcgataatcg tcctgtatcg
     1501 aaccatccgc ttcaacgctg tcttttgcgc ctaatccgtt ctcgacaata aataacggtt
     1561 tttgataacg atcccaaagc gtatttaaca gaacccgtaa tccaaccgga tcaatttgcc
     1621 acccccactc tgaacttttc agatgcggat tggggatcat attcagtatg ttgccctgcg
     1681 catttttatt aatgctttcg tcgtgggaaa cacaaccagt catgtaataa ctaaaagaga
     1741 tgaaatcgac ggtatgtttt aaatcttctg cgtcactttc agtcatctca atggtgatat
     1801 tgtggtcgcg gaagaaacgc tgcatatagc cgggatactg gccacgcgcc tgaacatcac
     1861 caaagaacat ccagcgccgg ttctcttcca tggcctgcaa catatcctgt ggctggcagg
     1921 tgagggggta aaccagccca ccgagaagca tattgccgat tttcgcttcg gggagcaggc
     1981 tatgacaggc tttaactgcc cgcgcactgg caaccagttg atggtggata gcctgataaa
     2041 cttccgcctc gccactctct tctgccagcc ccacgcccgt gaatggcgcg tgtaacgaca
     2101 tgttgatttc attaaacgtc agccataacg ccactttatg ttggtagcga gtaaagaccg
     2161 tgcgggcgta atgctcgaag tgatcgatga ccgctcgatt agcccaaccg ccgtagtttt
     2221 tcaccagccc atatggcatt tcgtaatggg ataacgttac cagcggcttg atccccgcct
     2281 gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc ggttcgactt
     2341 cgtctccctg agggaaaatt cgcgcccagg caatggaaat acgcagacag gtgaagccca
     2401 tctcggcaaa taacgcgata tcttccgggt aacggtgata aaaatcgatg gcgacatctt
     2461 tgatattctc tttccccagg atgcgcggtt ccatttttcc cattacgcca tgaggctgta
     2521 aatctgaggt cgagatccct ttgccatctt cctgccaggc accttccacc tgattggcag
     2581 ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc
     2641 gttagcgaat gatggataac agcggttcac ctgcgcttat ctgcgccgtg ccgtggggta
     2701 atacgtccgt aaaatcatcg ctattactga ttaataccgg cgtcgtcaga tcaaatccgg
     2761 cctcgcgaat agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac
     2821 ccacgttgac gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg ataccgacat
     2881 gaatcaggat ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca
     2941 acgaagcaat tcgacccgca accggagaac gcacttcacc aaccgagggc agaatggcaa
     3001 tacctttacc caacaggcca ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa
     3061 tctctcccgt catcggtgaa cagataccgc cctgctcagg tggtgtaata acctctggtg
     3121 ttttctcttg cggggcaccc tgcgctggct gacgtttagc ggtgatgaaa tgaagcatca
     3181 ccgtaccgac aaatgcgcaa ccgatggcaa tgacaccgcc aataacgctg gcccagacgg
     3241 tgaaatcaat tcccgttgac gggatggttt gcatgaaggt gaaaatactt ggcaaaccaa
     3301 aggagtagac tttcgtttgc gcgtagccaa tgatggtggc ccccaaagcc ccactgatac
     3361 aggcgataac aaaggggtac ttacgcggca ggttgacgcc atataccgct ggttcggtga
     3421 taccaaacag actcgtcaac gccgctgatc ccgccaccac ttttttctgc gcatcgcgtt
     3481 cgcagaggaa gacgccgagc gccgccccga cctgcgccat aatggcgggc attaacagcg
     3541 ggatcatggt gtcgtagccc agcacggtga agttattgat acacagcggc accaggcccc
     3601 agtgcagtcc gaacatgacg aagatttgcc agaagccgcc cattaccgcg cccgcaaatg
     3661 caggaaccgc ctgataaagc cagagataac cggcggcaat cagttcgctt atccaggttg
     3721 atagcggccc caccagcaga aaggtgacgg gtgtgataac catcagacat agcaatggtg
     3781 tgaagaaatt tttgattgcc gacggtaacc acgcattaag tcggcgttcc agaatgctgc
     3841 acaaccaggc agaaaaaata atgggaataa ccgatgacga gtaattcaac aatgtgaccg
     3901 gaatacccag gaaatccagc cccagcgcat ccgctttttg cccgttctcg aaagcagtca
     3961 gaattaatgg atgcactaac gctccaccaa tcaccatggc agtaaatgga ttaccgccga
     4021 agcgtttccc cgcggtgtat cccaggatta tcgggaagaa ccaaaacaag gcatcactgg
     4081 cgctgaataa aattaaataa gtaccacttt gttcggtcgt ccactgaaaa gtgagcgcca
     4141 gagccagcat acctttcaag atcccggttg ccgccatcaa accgatcaga ggcgtaaaaa
     4201 tacctgaaat aacataaaca aagcggttta gcagattacc tttatcatca ttttccggtg
     4261 cctgttgcgc tttttcgtca aggcctgcca cactgttaac cgccaggaag acatcggcca
     4321 catggttacc tatgaccacc tgaaactggc caccgctttc caccaccata ataataccgg
     4381 gggtcttttt cagtacctct gcttgcgctt tgctttcatc ctttaattta aaacgtaatc
     4441 gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc cccgactcct gcgactattt
     4501 ttctggctaa ctccgtcata acttgccctc taccgctttg cggcaaaact ccaaaaaaaa
     4561 acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt tcaggttttg cccgcttagt
     4621 gcggtaacaa tcctttactc agtaataata tttcagtgtt ctttgcgcac gcgctctata
     4681 tttatggcta aaaacataat ctctgcgggt gaaattttac gttgatactg caaaccaata
     4741 aaaatggcga tccgttccgc acattgccat gcttgcgggt aattttgttt tactgcttgt
     4801 tgtaatgatt catcactatc gttaattgaa gcatgttcaa gaatacgcca ggataaaaac
     4861 ttcagatgtg taaccagtcg ctgataactc aagctttctt cctggtaatt aaggctgaac
     4921 tgaaatttta ttaattgcag catttcgcgc attaactgcg tgacacctgc aacatcctcc
     4981 atatttccgc tcatttgggc actgaccaga tgcatggcaa taaagcccac ttcatctttc
     5041 ggtaactgca cgcccaaccg tttatcaata atggttaacg cttcttcccc tagctggaac
     5101 tctttcgggt aaagccgctg gatatcccac agcaacgggt tgggcagcaa cacgttttgc
     5161 tgaaagcgtt taatcgcaaa ctggcaat
//
LOCUS       DACAPB010000251         5154 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.253, whole genome shotgun sequence.
ACCESSION   DACAPB010000251 DACAPB010000000
VERSION     DACAPB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.253"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1399)
                     /gene="dtpA"
                     /locus_tag="IT904_004350"
     CDS             complement(<1..1399)
                     /gene="dtpA"
                     /locus_tag="IT904_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAP3194039.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGV"
     gene            complement(2010..2645)
                     /gene="nth"
                     /locus_tag="IT904_004351"
     CDS             complement(2010..2645)
                     /gene="nth"
                     /locus_tag="IT904_004351"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP3194040.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2645..3340)
                     /locus_tag="IT904_004352"
     CDS             complement(2645..3340)
                     /locus_tag="IT904_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAP3194041.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(3344..3964)
                     /gene="rsxG"
                     /locus_tag="IT904_004353"
     CDS             complement(3344..3964)
                     /gene="rsxG"
                     /locus_tag="IT904_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAP3194042.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIERRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(3968..5026)
                     /gene="rsxD"
                     /locus_tag="IT904_004354"
     CDS             complement(3968..5026)
                     /gene="rsxD"
                     /locus_tag="IT904_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAP3194043.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCAMLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5027..>5154)
                     /locus_tag="IT904_004355"
     CDS             complement(5027..>5154)
                     /locus_tag="IT904_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HAP3194044.1"
                     /translation="EQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1364 a         1322 c         1263 g         1205 t
ORIGIN      
        1 cgacaccaat ctgcaagaat acgcgaccat agacttccag tgacatcagc ggatcggtaa
       61 cgttatccgg cacagccatc atacccgcaa cataaccacc aatcaggttt gcaccggcag
      121 tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg agttgagcaa
      181 ccattgccag acccagacca gagatcatca gttccccgat gctctgcagg ccatagcttg
      241 cgaccagcca gcttacagac acgataccag cgtcagacgc gaatttcgca cccagcggca
      301 gaatcaggaa cgcaccagaa cacatcacca tgccgattgc aaacttggtt ggcatcggca
      361 gggtatcgcc catcttgtta tagatagcgg ccagaatcgg actaccgatg atgatccaga
      421 acgggttcag tgcctgatac tgctcaggtt ctacggccag acccagaatg gagtgctcaa
      481 cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctgtacagc acgaagaaga
      541 taatggcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca gcacctttca
      601 tcgcgaaggc ttctttaccg aagataacca cgataccgaa ggcaacaacg cccagcgcca
      661 tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat agcgatcagt gccacaacac
      721 caataatggt cagcagcagg ttacggtagt tgataggctc gaagtctggt tttgaaccgt
      781 actgtttaac ccagcgttgg cagaaggcga agttaacgat agtgatcagc aggcctacaa
      841 cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggc gtagcaatca
      901 tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca ccgtccagac
      961 gcgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct ttaaacaggc
     1021 cgttaccgac cgcaatagcc gccataccca tataaacgat accggcgtcg tgaccagacc
     1081 aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt acgcgtttag
     1141 tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa accagggcac
     1201 taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt ttaaccaggt
     1261 aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac tcaatcgaga
     1321 agatgagata gaacgccttc ggttgtttga aagcgttcaa actgacgctt tcagttggtt
     1381 tttggtttgc agtggacact tttacctctt tttttacatc ccatattaac gggggtgctt
     1441 tctcgcgtta tgtataaaaa acatacgcgc aattgttata gtgggagggg aaacggcagg
     1501 taatgttcac tatcttgcac tttcagacaa gtcctttgta ataatctgtt acatataact
     1561 gaggcgggga attcaaccat tccgggaaat gttatccagc gttaaatttt tcaaaaaacg
     1621 taacggcaga ttttttcact gcactgagag gggggtgatg gcttaacggc gatatgttct
     1681 gctatttagc gtttttacta gtgagatagt ccagtttctg aaaaatagcc agtgtaatgt
     1741 tttgtaggtc aatatttgtt gcgtgtgtaa atttaaatga ttttaacctg attcttgcaa
     1801 tgtttttaca ttgcgacagc atcgtgatct tgatcacgaa tgaatagaaa atttcgctat
     1861 taaaaaaaca attaacctgc atttttggtt atccgcaaac ggattacctg atgataacgc
     1921 taagcaatgg catcattgac gttaaaaaga tgttttaaaa gaggataaag aaaggttatc
     1981 aatggggtaa tcggtgttac cccttttctt cagatgtcaa ctttctcttt gtattcacaa
     2041 agatcttcaa taatacaaga gccacagcgg ggcttgcggg caatgcaggt ataacgcccg
     2101 tgcaggatca accaatggtg gcagtcgact ttaaactctg ccggaaccac tttcagtagc
     2161 ttttcttcta cctgttcgac gtttttcccc ggcgcaaatt gagtacgatt acaaacgcgg
     2221 aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg cagtgtttaa tacgacgttg
     2281 gctgttttac gacctacgcc gggcagggct tcaagcgcag cacgatcttc cggaacctcg
     2341 ccattatgct gctccagcaa gatacggcag gttttgatga tattttctgc tttgctgtta
     2401 taaagcccaa tcgttttgat ataggttttc accccttcaa cgcccagttc aagcatcgct
     2461 gcaggcgtat tcgccaccgg gtagagtttc gccgtcgcct tattaacact gacatcggtc
     2521 gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag gcgaactgaa attaagctcg
     2581 gtggtgggat gaggattgtt ctcacgcagg cgagtgagga tctccaggcg ttttgcttta
     2641 ttcatcagac attccctgtt tcaccgtttg gcaatgcacg ttctgcagct gcttcagcac
     2701 ggcgcttttt cattctttca tcaatcaggt attttcctgc cagcatcagt cccaggccaa
     2761 taaatgcacc tggtggcagc atcgccagca ggaaagggga gtcggtgtgg aaaatctcca
     2821 cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc accgtcaaac aatgtgccat
     2881 tgccgataat ttcgcgtagt gaacccagca cgaacatggc gcaggttgcg cccataccaa
     2941 ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt ggcggcgaag gcttcagcgc
     3001 ggcccacaac gatacagtta gtgacaatca gcggaataaa aatccctaat gattgataca
     3061 ggccaaaggc gtaggcgttg atcagcatct gtacagcgct gaccaccgag gcgatgatca
     3121 tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg acgcagcgtc gaaatggtca
     3181 ggttggtcag cgtcagtacc agcgtagtcg caagtcctaa acccagagcg ttagtggcag
     3241 tggacgtgac cgccaacaga ggacaaaggc cgagcaactg gaccagcgca gagttgtttt
     3301 tccacaaccc ctgaacaata acgtctttaa tttcgctcac gctttattct ccacaggcag
     3361 gaagttgaga aagttgtgcc ggtaacgtct gagcgtacaa tccggcgcgt tttaccgcat
     3421 taaccaccgc gcggggagta atcgtcgcgc cggtgaactg gtcgaaatca ccaccatctt
     3481 tcttcaccgc ccagtgcgca tcatctgcac cactgatttt tttacccgca aaatgggtga
     3541 tccagtcaga aaggcgccgt tcgattttat cgccaagccc tggcgtttcg tggtgctctg
     3601 tcacgcgcgt gccaagtacc gtgccgttaa aatcggctcc caccagcagc tgaatcgcac
     3661 cggaatagcc atctggcgcg gttgcttcca gaacggcggc taccggtttg tcatcctgtt
     3721 tggcgatgta aacccgatgc tcacctttac ctaactctgg cgcagttacc agatagcaac
     3781 tctgtgccag cgcattgtta tagcgttcgg ctggcagcac ctgatcaaat aacgcctttt
     3841 gttgcagact ggcctgttca gcaatcgtcg ttttggtcat ctggttgatg gccgcagtta
     3901 accctgttga acccgctgca aatagcgcca acgtaatgcc gtgttttcgg atagttttca
     3961 gcatggttta ccctttgcga tggccgtaga cgcgcggacg cgtgtagtaa tcgatcagag
     4021 gaaccgtgat gttcgccagc aggacggcaa aagccacgcc gtcaggatag ccgccgaaac
     4081 tgcggatcaa ccagactaat aagcccgcaa gcgcgccgaa aataagacga ccacgattgg
     4141 tcgtagaagc ggtaaccggg tcagtcaaaa taaagaatgc gccgagcatg gtcgctccag
     4201 acagcagatg aatttgcggt gctgccagtg tttctggtga gaacaaccag cccaacattg
     4261 cgcataacgc cagcgttact aagaagctga ggggaatatg ccagcgaatc gctttctgcc
     4321 atagcaacca tacgccgcca gccagccagg cgagatttac ccattgccaa ccagcgcccg
     4381 ccagaatacc gctgtagatc ggatattgca taatctgttc aaccgaatga ccggcacgga
     4441 cagaggtttt aaatgtatcc agcggtgtcg cctgactaat gccatcaata cctaagcgta
     4501 gtgtgttcat atcaccacca ctggcggtat gaccgctaaa aataacctgg atggcgtcga
     4561 taaaaccagg gatgttgacc gcaatttcat gtggcggtaa ccagctggtc atctgcacgg
     4621 ggaaggagat cagtaagacc acataaccaa tcattgccgg attaaacggg ttttgtccca
     4681 gaccgccata caactgttta gcgatgatca ccgcaaacac cgtacccagc acgaccatcc
     4741 accatggcgc gaggggggga atacttaccg ccagcaataa gcctgtcagc aatgctgagt
     4801 tatctttcaa cgttgcggct accgactgct tgcgtagttt gagtacgaga gcttcggcta
     4861 acagagcact aaccgatgcc aacaggatct gaacgagagt accccaacca aaaaaccaca
     4921 gttgcgctgc gattcctggc acggctgcga gcaacaccaa cagcataatg cgcgatgtct
     4981 ggcgctggtt atgggtataa ggggagctag ctattctgaa taccatttag tcctcgttta
     5041 caaccttctg ctgggcggct tttttggcct gagcgcgggc aatagccgcg gcaactgccg
     5101 ctttgcgcgg atcaacctgt tcttctggtt ctgcgttagt ctgttgctgt tcca
//
LOCUS       DACAPB010000252         5135 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.254, whole genome shotgun sequence.
ACCESSION   DACAPB010000252 DACAPB010000000
VERSION     DACAPB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.254"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..331
                     /gene="intI1"
                     /locus_tag="IT904_004356"
     CDS             <1..331
                     /gene="intI1"
                     /locus_tag="IT904_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAP3194045.1"
                     /translation="WFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITK
                     PATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDAL
                     PPLTSER"
     gene            534..884
                     /locus_tag="IT904_004357"
     CDS             534..884
                     /locus_tag="IT904_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAP3194046.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            904..969
                     /locus_tag="IT904_004358"
                     /pseudo
     CDS             904..969
                     /locus_tag="IT904_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1087..1851
                     /locus_tag="IT904_004359"
     CDS             1087..1851
                     /locus_tag="IT904_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAP3194047.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(2344..2928)
                     /locus_tag="IT904_004360"
     CDS             complement(2344..2928)
                     /locus_tag="IT904_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3194048.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(2928..4166)
                     /locus_tag="IT904_004361"
     CDS             complement(2928..4166)
                     /locus_tag="IT904_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3194049.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(4163..5068)
                     /gene="mph(A)"
                     /locus_tag="IT904_004362"
     CDS             complement(4163..5068)
                     /gene="mph(A)"
                     /locus_tag="IT904_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAP3194050.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1049 a         1599 c         1588 g          899 t
ORIGIN      
        1 gtggttctgg gtttttgcgc agcacacgca ttcgaccgat ccacggagcg gtgtcgtgcg
       61 tcgccatcac atgtatgacc agacctttca gcgcgccttc aaacgtgccg tagaacaagc
      121 aggcatcacg aagcccgcca caccgcacac cctccgccac tcgttcgcga cggccttgct
      181 ccgcagcggt tacgacattc gaaccgtgca ggatctgctc ggccattccg acgtctctac
      241 gacgatgatt tacacgcatg tgctgaaagt tggcggtgcc ggagtgcgct caccgcttga
      301 tgcgctgccg cccctcacta gtgagaggta gggcagcgca agtcaatcct ggcggattca
      361 ctacccctgc gcgaaggcca tcggtgccgc atcgaacggc cggttgcgga aagtcctccc
      421 tgcgtccgct gatggccggc agcagcccgt cgttgcctga tggatccaac ccctccgctg
      481 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acaatggagg
      541 tggtagccga gggtgtggaa acacccgact gccttgcgtg gttgcggcag gcgggttgcg
      601 acacggtgca gggtttcctg ttcgccaggc cgatgccggc ggcggccttc gtcggcttcg
      661 tcaaccaatg gaggaacacc accatgaacg ccaatgaacc gagcaccagt tgctgcgtgt
      721 gctgcaagga aatcccgctc gatgccgcct tcacgccgga aggggccgag tacgtggagc
      781 atttctgcgg gctggagtgc tatcagcgct tccaggcgcg ggccagcact gcgaccgaaa
      841 ccagcgtcaa accggacgct tgtgattcgc cgccgtcagg ttgaggcata ccctaactgg
      901 attgtcattt tcagaagacg actgcaccag ttgattgggc gtaatggctg ttgtgcagcc
      961 agctcctgac agttcaatat cagaagtgat ctgcaccaat ctcgactatg ctcaatactc
     1021 gtgtgggctc tgttgcaaaa atcgtgaagc ttgagcatgc ttggcggaga ttggacggac
     1081 ggaacgatga cggatttcaa gtggcgccat ttccagggtg atgtgatcct gtgggcggtg
     1141 cgctggtatt gtcgctatcc gatcagctat cgcgaccttg aggaaatgct ggcggaacgc
     1201 ggcatttcgg tcgaccatac gacgatctat cgctgggtcc agtgctacgc cccggagatg
     1261 gagaagcggc tgcgctggtt ctggcggcgt ggctttgatc cgagctggcg cctggatgaa
     1321 acctacgtca aggtgcgggg caagtggacc tacctgtacc gggcagtcga caagcggggc
     1381 gacacgatcg atttctacct gtcgccgacc cgcagcgcca aggcagcgaa gcggttcctg
     1441 ggcaaggccc tgcgaggcct gaagcactgg gaaaagcctg ccacgctcaa taccgacaaa
     1501 gcgccgagct atggtgcagc gatcaccgaa ttgaagcgcg aaggaaagct ggaccgggag
     1561 acggcccacc ggcaggtgaa gtatctcaat aacgtgatcg aggccgatca cggaaagctc
     1621 aagatactga tcaagccggt gcgcggtttc aaatcgatcc ccacggccta tgccacgatc
     1681 aagggattcg aagtcatgcg agccctgcgc aaaggacagg ctcgcccctg gtgcctgcag
     1741 cccggcatca ggggcgaggt gcgccttgtg gagagagctt ttggcattgg gccctcggcg
     1801 ctgacggagg ccatgggcat gctcaaccac catttcgcag cagccgcctg atcggcgcag
     1861 agcgacagcc tacctctgac tgccgccaat ctttgcaaca gagcccgccg tgctagtctg
     1921 ctcggtgatg gtggagtgaa gccaacccgc aatcgggtta tgaatctgca tcgcgattcg
     1981 caatcagctg tctcttgagc atgtcgaact cctgcgatgt tatctcgccg tgctcgtgct
     2041 gctacaccaa cttgtccaac tcatccgcca caccgctccc caccacctgc gatggccagg
     2101 ttgtttgctg ggcgcattgg cggagtatcg tctcgacccg ggacacccac accggcacaa
     2161 ccttacggga gtgattcact gtcaaagaat cggcccggtg ctctgacgca agtatcggga
     2221 tggtcaccat ttgtaagccg tagacctgag tggtgatcaa gacttcgata ccaccgaccg
     2281 taccggtact aatcgacgac ggtcgtgttc gtcgcctgcc gcagggactc tgcacacctc
     2341 cgtttacgca tgtgcctgga ggagttggaa atcgtcgtgt tcgggaaaca ttaaacacag
     2401 gatggcagcg atctgagcca gcacatgatc agctagctca ccatccggat cgacggccca
     2461 ctgcatcgtc gcgccagcga tgaccgagtg caggagcaac tcagctgccg caggagcacc
     2521 tgggggcagt cgcttgcgga tcccctccac caccgcgcgg ttccgctgga tcgcaagcgt
     2581 gcgtagctcc ggcacctgga gctcgtacca ggagatgaga tagttcaccg agaagtcgtt
     2641 gcgagtgttc atgctccgaa cgagcacctg caaaaattcc cagagccctt gcggccctgc
     2701 gcctatcggt atcgcattca ggtaatgccg cacctgctcg acgccgcgct ccatcatcct
     2761 caccagcagc gtatcgcggt tggtgaagcg ctggattaac gctgcgcggg agagccccac
     2821 ctcctttgct actccgctga gcgtgaactc tatgggaccg caacgcttca gcactacggt
     2881 ggcggcctcg agtacctcgt catcggactt gagcttgggg cggggcatca gtgttcacct
     2941 tctgtatggg ttggggcgga ggctgtggct gccgccgcca ttgtagcaaa ttgaagacgg
     3001 agcgagagta gagccacgag ccccgcaaac acggccgata caacgaggcc agggagcgga
     3061 ccagcaaggt cgacaaacgc gccggccgca agcataacca tgggcgaggc tgacagcatc
     3121 accgccgaga ccgtgccgag tacccggccg agaagttctg gcggcgtgcg gttgtagatg
     3181 gcagcgttga gaatgggaga gactgagccg gtcagcagtc ccacgagcgc gcccaacaac
     3241 atcagcaccg gcacgcctgg caactgtgaa agcagaagcg agcccaccgc agagccacaa
     3301 aatgccaccg ccagccagtt ctgcgctgat atccgggcgc cgaccgacgc atgaatggca
     3361 atgccaagga gaccaccagc ccccatcatt gaggagaaca gcccgagctc tgctacttgg
     3421 cgtcctgcat ctacaaacag cgcaggcatg atgacgctgc cgttggcgcc aacgatgccc
     3481 acgaagatca tcactatacc aaagagaggg cgcagcaggg gttcgctcca gagaaaagcg
     3541 acgccggcgc gcatggagag agtcgccgtc gtggtcatcg tccgagcggc acgcgcggga
     3601 agcacccacg cgccgagcag acctgcaagg acggagcaga acgccgtcag cccgagcgtt
     3661 ggcgcagcgc caagcaggcc gattgcggcc cccccaaggg ccgggccacc tagaatcgcg
     3721 acgttcccga tcaccgcttt cagtgacgag acgcgctcaa cggagagccc ggcgacgtgg
     3781 ccgagtttgg gcagctcact gtcctgcgcg gccataccgg gtgcgtcgaa cgcggcaccg
     3841 agcaccacgc aagcgatcag cccagtgttc gagagggcgc caacggcatc gagcagtggg
     3901 atgctcgcca tggccacgcc gcccaccaca cccgagatca atgcgacggg cgcgcgcccg
     3961 aaccgatcga cgaggccacc accaacccac gcgccgatga tggtcgcgat gacgctgcta
     4021 gcggccgtgg cgcccgccca ggccgcgctc tttgtatgag acaggacgaa ccatggaagc
     4081 gcgagggccg ccaccgcgtt gccgatccgg aagagaaagg tcgccgcgaa cagcgtcgcg
     4141 agcgggctat atcgacgttc gctcattccg ctgcggcgag ctgcgccttc gccgcagcga
     4201 ggtactcttc gttacccgag tcgagggcga agagtgcgta ggtgaccgcc ccgaacgcaa
     4261 ggcgctccgc gatgtggtgg gcgagccgcg gccacacccg gccaccggcc gcttcatacg
     4321 tgaggaggag cttcgcgagc ccctcttcac caaagaccat aaggtgcgcg gccatgtcga
     4381 tggcagggtc atcaacgcgg gcctcgctcc agtcgatcat cccgctgacg cgctccgtgt
     4441 tgtcgatgag cacatggccc acgtagagat cgccatgcac caccacggag aaatctggcc
     4501 acgacgaatc gtcgtcgagc cagcgctgcc accggtggag gcgcttgtcg ttcaccacga
     4561 actcgcgtcg gacgcggtca acgtcgtcgg ccaccttctg acgggcctgc gtcggtgtac
     4621 ggatgagcat ccccgcatcc acggcggcgg aaatggggac ggcatgcagg gcggcgagcg
     4681 cggtcgcgaa gctctccgcg aagacctccg agtcctgcgg cacgacccag tcgggcgtgg
     4741 acgaaccagg ctggatgacc atcgcagtcg agtcttcgag catgggatag gcaacgagct
     4801 cggcgttggc cacgcgccag tccggcaccg cgaacggcag gcgattcttg agcattgcca
     4861 gcacccgcgc ctctggttcg accttcgcgc ttacctcggc tcggcgcggg atgcgcagca
     4921 cccaccgacg tccatcgtcg acggtggcga tcacgatcct atagtcgagc ccaagctcat
     4981 tgacagtcag cgggccatgg agcttgagcc catgtcgggc tgcaagtgcg tacagttggg
     5041 aggtatcggc ggtcgtgact acggtcatga ttcactcctg agggcttgac gggtttagcc
     5101 acctaaatgt aacagtcacg tcggttatat tcaat
//
LOCUS       DACAPB010000253         5123 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.255, whole genome shotgun sequence.
ACCESSION   DACAPB010000253 DACAPB010000000
VERSION     DACAPB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.255"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..808
                     /gene="ada"
                     /locus_tag="IT904_004363"
     CDS             <1..808
                     /gene="ada"
                     /locus_tag="IT904_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAP3194051.1"
                     /translation="ITHVCRLLEQETPVTLEALADHVAMSPFHLHRLFKATTGMTPKA
                     WQQAWRARRLRESLAKGESVTTSILNAGFPDSSSYYRKADETLGMTAKQFRHGGENLA
                     VRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADNAPADLMFQQHV
                     REVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVR
                     AVASACAANKLAIVIPCHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER"
     gene            808..1458
                     /gene="alkB"
                     /locus_tag="IT904_004364"
     CDS             808..1458
                     /gene="alkB"
                     /locus_tag="IT904_004364"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAP3194052.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAGKKE"
     gene            1534..3177
                     /gene="yojI"
                     /locus_tag="IT904_004365"
     CDS             1534..3177
                     /gene="yojI"
                     /locus_tag="IT904_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAP3194053.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            3395..5041
                     /gene="mqo"
                     /locus_tag="IT904_004366"
     CDS             3395..5041
                     /gene="mqo"
                     /locus_tag="IT904_004366"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAP3194054.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQAMLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT         1183 a         1265 c         1462 g         1213 t
ORIGIN      
        1 aatcacccac gtgtgtcgac tgctggaaca ggaaacgcct gtaacgctgg aagccttagc
       61 cgaccatgtg gcgatgagtc cgttccatct gcatcggttg tttaaagcta ctaccggaat
      121 gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa
      181 aggggagagc gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta
      241 tcgcaaagct gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa
      301 tctggcggtg cgttacgcgc tggctgattg tgagctgggt cgttgcctgg tggcagaaag
      361 cgagcggggg atttgcgcga tattgctggg cgatgatgac gcgacactaa tcagcgagtt
      421 gcagcagatg tttcccgctg ccgacaacgc gcctgccgat ctgatgtttc agcaacatgt
      481 gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggacat
      541 tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgcggtga
      601 aaccgtcagt tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt
      661 tgccagcgcc tgtgccgcca acaagctggc tatcgtaata ccctgtcatc gagtggtccg
      721 tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg
      781 ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg
      841 caagagccac tggcggctgg agcggtaatt ttgcaccgtt ttgcttttaa cgctgcggag
      901 caactaatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcacc
      961 cccgggggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgagc
     1021 catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc
     1081 atgccacaga gttttcataa tttatgtcaa cgtgcggcta cggcggcggg ctatccagat
     1141 ttccagccag atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat
     1201 caggataaag acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct gggcttaccc
     1261 gcgatttttc aatttggcgg cctgaaacga aatgatccgc tcaaacgttt gttgttggaa
     1321 catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg tattcaaccg
     1381 ttgaaagcgg ggtttcatcc actcaccatc gactgccgct acaacctgac attccgtcag
     1441 gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac
     1501 tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg
     1561 cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc
     1621 attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg
     1681 gtgttgccgg agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa
     1741 ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag
     1801 cggattctgg atactcacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg
     1861 gggttaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc ggaactggtg
     1921 caggggatca ttctcactat cggttcagcg gcgtatctgt ggatgctgtc gggcaaaatg
     1981 ttgctggtaa cggctatctg gatggcgatc accatctggg gcggttttgt gctggtggcg
     2041 cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag acaagctgta cacggatttt
     2101 caaactgtac ttgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg
     2161 tttaacaacc tctacattcc tgatgcgcaa gagtatcgcc atcatattat tcgcgcagac
     2221 accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc aatcggcctg
     2281 gtgttctgga tggcgaacag cctcggttgg gctgatacca acgttgccgc gacctattcg
     2341 ttgacgcttt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg
     2401 ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagca
     2461 gagtttccgc gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg
     2521 tttgcttatc aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc
     2581 gagctgctgt ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgttg
     2641 acgggcttgt atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc
     2701 gaacaaccgg aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt
     2761 gatcaactgc tggggccgga gggtaaaccc gctaacccgc aactggttga gaagtggctg
     2821 gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag
     2881 ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat
     2941 attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat
     3001 caggtgttgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat cagtcatgat
     3061 gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca acttagcgag
     3121 ctgacgggcg aagagcgcga tgctgcttcg cgtgatgccg ttgcccggac ggcataatat
     3181 tacgacgctg acctggcgga ctgctgccgt caggtcaata tgctaaaaat cccttcattt
     3241 ctaataaccc tataatttat caacgaaatt ataatgtttc taaaattaga atataattta
     3301 taaacattat ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct
     3361 gcaataaagc gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt
     3421 ctcgatggcc gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga
     3481 gcaggaaact gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta
     3541 tttacgcgag ctggagcctg aatggtcgat gaccatggtg gagcgcctgg agggtgtcgc
     3601 gcaggagagt tcgaacggct ggaataacgc cggaaccggg cattctgcac tgatggaact
     3661 gaactacacc ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa
     3721 cgaagcattt cagatttccc gccagttctg ggcgcaccag gttgagcgcg gcgtgctgcg
     3781 tactccgcgt tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa
     3841 cgttaatttc ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat
     3901 gcgttactct gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg
     3961 cgatccgcaa cagaaagtgg cagccacgcg tacggaaatt ggtaccgatg tgaactacgg
     4021 cgagatcacc cgccagttaa ttgcttcctt gcagaagaaa tctaacttct cgctgcaact
     4081 cagcagcgaa gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc
     4141 cgatctgaaa aatggcactg cacagaacat tcgtgccaaa tttgtcttta tcggcgcggg
     4201 cggtgcggcg ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg
     4261 tttcccggtg ggcggacagt tccttgtttc ggaaaacccg gacgtggtta atcaccatct
     4321 ggcgaaggtt tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga
     4381 tacccgcgtt ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac
     4441 caaattcctc aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt
     4501 gatgccgatg atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca
     4561 ggtgatgttg agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaggcgaa
     4621 aaaagaggac tggcgtttgt ggcaagcggg gcagcgcgtg cagattatca agcgtgatgc
     4681 cgagaaaggt ggcgtactgc gtctgggtac tgaagtcgtc agtgaccagc aagggaccat
     4741 tgccgcgctc ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttggatct
     4801 gctggaaaaa gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta tgttgaaagc
     4861 gatcgttccg tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt
     4921 gcagtacacc agcgaagtgc tggggctgaa atacgacaag ccgcaggcag cagatagtac
     4981 gccgaaaccg cagttgaaac cgaaacccgt tcaaaaagaa gtggcggata ttgcgttgta
     5041 atgatgtgcc acatccggca tggtatgccg gggatgtggc gtatgctgat aagacgcgcc
     5101 agcgtcttat caggcaaccg gct
//
LOCUS       DACAPB010000254         5100 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.257, whole genome shotgun sequence.
ACCESSION   DACAPB010000254 DACAPB010000000
VERSION     DACAPB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.257"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..215
                     /locus_tag="IT904_004367"
     CDS             <1..215
                     /locus_tag="IT904_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAP3194055.1"
                     /translation="RYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTH
                     LYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            220..609
                     /gene="csgE"
                     /locus_tag="IT904_004368"
     CDS             220..609
                     /gene="csgE"
                     /locus_tag="IT904_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAP3194056.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            634..1050
                     /gene="csgF"
                     /locus_tag="IT904_004369"
     CDS             634..1050
                     /gene="csgF"
                     /locus_tag="IT904_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAP3194057.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1077..1910
                     /gene="csgG"
                     /locus_tag="IT904_004370"
     CDS             1077..1910
                     /gene="csgG"
                     /locus_tag="IT904_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HAP3194058.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1974..2465)
                     /locus_tag="IT904_004371"
     CDS             complement(1974..2465)
                     /locus_tag="IT904_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP3194059.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2567..3121)
                     /locus_tag="IT904_004372"
     CDS             complement(2567..3121)
                     /locus_tag="IT904_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3194060.1"
                     /translation="MNEFSILCRVLGSLYYRQSQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3145..3882)
                     /locus_tag="IT904_004373"
     CDS             complement(3145..3882)
                     /locus_tag="IT904_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAP3194061.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3937..4875)
                     /gene="ghrA"
                     /locus_tag="IT904_004374"
     CDS             complement(3937..4875)
                     /gene="ghrA"
                     /locus_tag="IT904_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAP3194062.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
BASE COUNT         1373 a         1289 c         1183 g         1255 t
ORIGIN      
        1 atcgttataa cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata
       61 agctgcgtat cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata
      121 cggttaaaac gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag
      181 cggtttcctg ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg
      241 attgtggcgg cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc
      301 ccgggattgc taactgacca tactgtttca tctattggcc atgattttta ccgagccttt
      361 agtgataaat gggaaagtga ctatacgggt aacttaacga ttaatgaaag gcccagtgca
      421 cgatggggaa gctggatcac tataacggtc aatcaggacg ttattttcca gactttttta
      481 tttccgttga aaagagactt cgagaaaact gtcgtctttg cactgattca aactgaagaa
      541 gcactaaatc gtcgccagat aaatcaggcg ttattaagta cgggcgattt ggcgcatgat
      601 gaattctaaa taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta
      661 ctcatgctta tttcgccatt aagttgggct ggaaccatga ctttccagtt ccgtaatcca
      721 aactttggtg gtaacccaaa taatggcgct tttttattaa atagcgctca ggcccaaaac
      781 tcttataaag atccgagcta taacgatgac tttggtattg aaacaccctc agcgttagat
      841 aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc
      901 ggtaaaccgg gccgcatggt gaccaacgat tatattgtcg atattgccaa ccgcgatggt
      961 caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat ccaggtttcg
     1021 ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa ataatcatgc
     1081 agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc gccccgccta
     1141 aagaagccgc cagaccgaca ttaatgcctc gtgctcagag ctacaaagat ttgacccatc
     1201 tgccagcgcc gacgggtaaa atctttgttt cggtatacaa cattcaggac gaaaccgggc
     1261 aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc gccacggcaa
     1321 tgctggtcac ggcactgaaa gattctcgct ggtttatacc gctggagcgc cagggcttac
     1381 aaaacctgct taacgagcgc aagattattc gtgcggcaca agaaaacggc acggttgcca
     1441 ttaataaccg aatcccgctg caatctttaa cggcggcaaa tatcatggtt gaaggttcga
     1501 ttatcggtta tgaaagcaac gtcaaatctg gcggggttgg ggcaagatat tttggcatcg
     1561 gtgccgacac gcaataccag ctcgatcaga ttgccgtgaa cctgcgcgtc gtcaatgtga
     1621 gtaccggcga gatcctttct tcggtgaaca ccagtaagac gatactttcc tatgaagttc
     1681 aggccggggt tttccgcttt attgactacc agcgcttgct tgaaggggaa gtgggttaca
     1741 cctcgaacga acctgttatg ctgtgcctga tgtcggctat cgaaacaggg gtcattttcc
     1801 tgattaatga tggtatcgac cgtggtctgt gggatttgca aaataaagca gaacggcaga
     1861 atgacattct ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa
     1921 agccggaatt atcatccggc attttactgt ttaagcgaaa gcgtgagtgc ccctcacgct
     1981 ttatttttga tttcctgctc acggtgcgcc gtctttgcac tacgcgccgc cagccacagg
     2041 ccactgtttt tcattgcgta accaaatatc agcccaagcg ccagcgaagg taacaccagt
     2101 ttccagtcac cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat
     2161 gaaagcagca gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca
     2221 taaccgagaa tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac
     2281 accacgccac taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg
     2341 caggcaaagt aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca
     2401 gccacccatc cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg
     2461 ttcatgccgc gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat
     2521 gcaacaacaa attatccgtt gcagttatga gtgacgtaga tcacacttat tcttcagaat
     2581 cttcttccag ctcgtcccac attgcactaa tggcatcgcg ggttagcggt gccatggtgc
     2641 gccagaaagg cgtggttgca tgggcctcca ctttgccaag gaacgcacca caccagggta
     2701 acagatactc actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt
     2761 ccagccagga agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg
     2821 gcatccctcg ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg
     2881 cgctacgata tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag
     2941 agacttgcgc catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg
     3001 gccagttcgc agccagtttc ccctcacgaa tcagggtaaa cagcggcacc agtaaaggat
     3061 cttgcgattg gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca
     3121 ttaatatatt tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc
     3181 gcgagattca aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctggcgg
     3241 aaaatctacc gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt
     3301 gtgagaatcc gagcctaatg ccacccaacc tccagcatca cgtaccgctg cggctaccgc
     3361 acgacagttg tcttcactgc ccttacgtga gtgtaaaaat gaagaattat tgatttccag
     3421 cgccacctga tgtttcgcgg ctgcttcagc aacggctttc acatctattt catatttggg
     3481 atttccggga tggcttatta tatgcacatt gccgctggcg atagtggcga tcatcgcttg
     3541 tgtattggtc gctttgtcat gtggtgcaaa aaccggctca tgaaaaccgg caataatcag
     3601 atccagcgag tcaaacattt taccgctgca atcaatttca ccatcaacat ttttaatgtt
     3661 agcttcgatg ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt
     3721 aatgaagtgc cagtgatgcg gagcatcttc catatccggg ccatgatcgg tgatcgcaaa
     3781 aagtttaatg cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg
     3841 tgtgctggca acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca
     3901 attcccgcca aggatagcag gaatcctgat gctttattag tagccgcgtg cgcggtcgac
     3961 ttgcccgcag accttctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac
     4021 agcttcagcg ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg
     4081 ccagagcgga ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt
     4141 aactttcccg ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc
     4201 caggttgagg agatacgcgc catccggtaa tttttcgagt aattgttgat taataatgcc
     4261 gacggtttca ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc
     4321 agacagttct tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact
     4381 ccagcaacgc agcggaaagc gccaggtttg cagactctga gcaactttac tgcccagtac
     4441 gcctgcgccc aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca
     4501 atgcgaacta ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg
     4561 actgacagca tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg
     4621 aacagaaggg ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc
     4681 ggccccgagt gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg
     4741 ccagactaaa gcataatcag cagagtcatt atctccgctt ttccatgctc tgactcttgc
     4801 ctgaggaata gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata
     4861 aaagatgata tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg
     4921 ttatgatcta caaaagccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat
     4981 ttcgcttatt aaaactgcat attgattgaa gtttgaataa acgcgcgatt ttttaaaaaa
     5041 gtttgttgac ctcaggtcat gatttcccta aattagcgcc cgttccagca agacaggaac
//
LOCUS       DACAPB010000255         5058 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.258, whole genome shotgun sequence.
ACCESSION   DACAPB010000255 DACAPB010000000
VERSION     DACAPB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.258"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..435)
                     /locus_tag="IT904_004375"
     CDS             complement(<1..435)
                     /locus_tag="IT904_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP3194063.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAP"
     gene            complement(447..1496)
                     /gene="glnL"
                     /locus_tag="IT904_004376"
     CDS             complement(447..1496)
                     /gene="glnL"
                     /locus_tag="IT904_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAP3194064.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(1782..3191)
                     /gene="glnA"
                     /locus_tag="IT904_004377"
     CDS             complement(1782..3191)
                     /gene="glnA"
                     /locus_tag="IT904_004377"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAP3194065.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3564..>5058
                     /gene="typA"
                     /locus_tag="IT904_004378"
     CDS             3564..>5058
                     /gene="typA"
                     /locus_tag="IT904_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP3194066.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVA"
BASE COUNT         1190 a         1328 c         1349 g         1191 t
ORIGIN      
        1 tggcgcttcg ccgatgatat cggtcgttgg gccgttaagc tgaatattac gcggctgctg
       61 ctgttcctgg taatgactga tagcgcgctc aaccagcgcc actgcttcgt cgatatcaaa
      121 cggtttgggc agataatcaa acgccccttg ttgataggcg ctgacggcag catccagatc
      181 ggaatgtgcg gtcataatga tgaccggaag cattggatgg cgctgtttaa tctgcttgag
      241 cagcgccagc ccgtccattc ccggcatacg gatatctgaa agcagcacat ccggcgtttt
      301 gctcgccagc gcctccagta cttccgcgcc gttctcaaat gtcgtacagg ttaaacccgc
      361 tccagcgagc gcacgttcaa gcacccaacg gatggaacta tcgtcatcga ctacccagac
      421 tatccctcgt tgcataaacg tcacctttat ttcctgatag gcaggtaaac cgagaactcg
      481 gtatgccctg gccaactggt aaattcaatt ttgcctgaat gctgatcaat caaattacga
      541 gcgatggata agccaagccc ggtgccacct tcgcggccgc tgaccatcgg gtaaaacagc
      601 gtatcctgca aatgaggcgg aatgcccggc ccgttatctt ccacatcaat ccgcgccgcc
      661 agccggtagc gctcgccgtg taaggtcagt tgaaacgcgg tgcgggtacg cagaatgatt
      721 tcaccgcctt ccggccccag cgcctgtagc gcattgcgca caatattcag caagacctgt
      781 tcaatttgat ccgggtcgtg cgccagttcc ggcaggctgg gatcgtaatc acgaatcaac
      841 cgcacgttgt ccggcagttc catcgacacc agcgttacca cgcgttcagc cactttgtga
      901 atactttcgg taacgcgcgt accgggcagc tgcggcccca acagacggtc gaccagattt
      961 cgcagccggt ccgcctgttc gataatcact ttggtatatt cgagtagtga tgggtcaggt
     1021 aacgctttgc tgagcagctg cgccgcgcca cgtaaaccgc caagcggatt tttaatctca
     1081 tgtgccaggc cgcgcactaa atcacgggca gcaacctgct gggcgtgctg tagctgttcc
     1141 tgacttaagc ggcgctggtt atccatcgga gccatctcca gcaggatcat gccgtccggc
     1201 atacgctggg ccgtcacaga aaggatatgc gagcgcccgt cgatgaccag cgtcacttcg
     1261 ttatcggtaa aaccttgccc cgcctccaga ctttcttgca tcagctcgat atttaatgag
     1321 aagtagctca acagttccgg taacggtgta ccaaacaatt tgcgggagct ttgggcgagc
     1381 agttgttgcg cggcggggtt ggcgtaatgg atcgccaggt tgtcatcgat taacaaaata
     1441 ctgttaatca gcgagttgag gatctgccca gcatcgggct gcgtgcctgt tgccataaag
     1501 cagtctcctg aacaggttgc accattttag tgcattatag ctttttacgg ataaaaagcg
     1561 cgaagcatca gagaattgac ggagaaaaaa gcccatgcag agatgggcta cagatagctg
     1621 acaaacttca cgttggagag cgactcacct gctccggcct accacatcat caccgtgtag
     1681 gccgggtaag acgcatttgc gtcgcatccg gcgtgatgtg cttgccaccg atcccaggcc
     1741 tgccagagac aggcgaaaag tttccacggc aactaaaaca cttagacgct gtagtacagc
     1801 tcaaactcta ccggatgcgg agtcatacgc acgcggtcat cttcttcgcg acgcagtgcg
     1861 atgtacgcat caattgcttc gtcagtgaac acgccaccgg ctttcaggaa ctcgcggtcc
     1921 agatccagtt cgttcagtgc ttcttccaga gagcctgcaa cctgtgggat ctctttcgct
     1981 tcttctggcg gcaggtcata caggtttttg tccatggctt cgcccggatg gatcttgttc
     2041 ttgataccat caagaccggc catcagcagg gcagcaaagc acaggtacgg gttagctgcc
     2101 ggatccggga aacgtacttc gatacgacgt gctttcggag aagaaaccac cggaatacgg
     2161 atagacgcag aacggttacg cgcagagtaa gccagcatta ccggtgcttc atagcccggg
     2221 accagacgct tataagagtt ggtggtcggg tttgccaggg cgttaatcgc tttagcgtgt
     2281 ttgattacgc cgccaatgta gtacagcgcc tgctcagaca gacctgcgta tttgtcgcct
     2341 gcgaacaggt taacgccgtt tttagacaga gacatgtggc agtgcatacc ggagccgtta
     2401 tcaccgaaca tcggttttgg cataaaggtc gcggttttac cgaagcggtg cgctacgttg
     2461 tgcacaacat atttgtagat ctgaatttcg tcagcttttt tggtcatggt attgaagcgg
     2521 gtagccactt cgttctgacc agcagtcgct acttcgtggt gatgggcttc aaccaccagg
     2581 cccatctgtt ccatcaccag acacatttca gaacgaatat cctgagcaga gtctaccggc
     2641 ggaaccggga agtaaccgcc tttcactgcc ggacggtgac ctttgttacc accttcatat
     2701 tgggtggagg agttccatgc gccttcgata tcgtcgatag caacgtgaga accggagata
     2761 gatgatccga aacggatgtc atcgaacagg aagaattcag gttctggccc gaacagtacg
     2821 gtgtcggcaa tgccagtgga acgcaggtaa tcttcggcgc gcttcgcaat ggagcgcggg
     2881 tcacggtcat agccttgcag ggtgccaggt tcaaggatgt cgcaacggat aatcagggtg
     2941 gagtcggcga agaacgggtc aatcactgcg gtggatgcgt ctggcatcag caccatgtcg
     3001 gactcgttaa tgcctttcca gccgccaatc gaggagccgt caaacatttt gccttcttcg
     3061 aagaattcag cattcacctg atgagcaggg atagtgacgt gctgttcttt acctttagta
     3121 tcggtgaagc gcaaatcaac aaacttcact tcgtgctcgt tcagcatcgt cagtacgtgt
     3181 tcagcggaca tactttaact ctcctggatt ggtcatggtc gtcgtggtaa cgaaatctgc
     3241 aattattttg gccgtgtcgc cgtaaaaaag ataaagcgaa atctgtgcca acttttaaat
     3301 tgcccctaaa aggcgttatc atgcgcacca tcgtgcaaaa gggctgcacc acgatgtgaa
     3361 tgttgcacca atatagtgct tcaatggaaa cattaagcac catgttggtg caatgacctt
     3421 tggataaccc tttttatgct ccgtgaaagc gatcacaaag ggactctgca atacttgttt
     3481 gcggaggatg tttgtgatcc tgttttgtag tgcgattaat ccgtgtacaa taacgcgcta
     3541 tttctaatgc ctgaggcaaa gttgtgatcg aaaaattgcg taatatcgcc atcatcgcgc
     3601 acgtagacca tggtaaaacc accctggtag acaagctgct ccaacaatcc ggtacgttcg
     3661 actctcgtgc cgaaacccaa gagcgcgtga tggactccaa cgatttggag aaagagcgtg
     3721 ggattaccat cctcgcgaaa aacaccgcta tcaaatggaa tgattaccgt atcaacatcg
     3781 ttgatacccc ggggcacgcc gacttcggtg gtgaagttga acgtgtaatg tccatggtag
     3841 actcagtgct gctggtggtt gacgcatttg acggcccgat gccgcaaacg cgcttcgtaa
     3901 ccaaaaaagc gtttgcttac ggcctgaagc cgattgttgt tatcaacaaa gttgaccgcc
     3961 ctggcgcgcg tcctgattgg gttgtggatc aggtattcga tctgttcgtt aacctcgacg
     4021 cgaccgacga gcagctggac ttcccgatcg tttacgcttc tgcgctgaac ggtatcgcgg
     4081 gtctggacca cgaagatatg gcggaagaca tgaccccgct gtatcaggcg attgttgatc
     4141 acgttcctgc gccggacgtt gaccttgacg gtccgttcca gatgcagatt tctcagctcg
     4201 attacaacag ctatgttggc gttatcggca ttggccgcat caagcgcggt aaagtgaagc
     4261 cgaaccagca ggtcactatc atcgatagcg aaggcaaaac ccgcaacgcg aaagtcggta
     4321 aagtgctggg ccacctcggt ctggaacgta tcgaaaccga tctggcggaa gctggcgata
     4381 tcgttgcgat cacgggcctt ggcgaactga acatttctga caccgtttgc gacacgcaaa
     4441 acgttgaagc gctgccggca ctctccgttg atgagccgac cgtttctatg ttcttctgcg
     4501 ttaacacctc gccgttctgc ggtaaagaag gtaagttcgt aacgtctcgt cagatcctgg
     4561 atcgtctgaa caaagaactg gtacacaacg ttgcgctgcg cgtagaagaa accgaagacg
     4621 ccgatgcgtt ccgcgtttct ggtcgtggcg aactgcacct gtctgttctg atcgaaaaca
     4681 tgcgtcgtga aggtttcgaa ctggcggtat cccgtccgaa agttatcttc cgtgaaatcg
     4741 acggtcgtaa acaagagccg tatgaaaacg tgactctgga cgttgaagaa cagcatcagg
     4801 gttctgtaat gcaggcgctg ggcgaacgta aaggcgacct gaaaaacatg aatccagacg
     4861 gtaaaggccg cgtacgtctc gactacgtga tcccaagccg tggtctgatt ggcttccgtt
     4921 ctgagttcat gaccatgact tccggtactg gtctgctgta ctccaccttc agccactacg
     4981 acgacgtacg tccgggtgaa gtgggtcagc gtcagaacgg cgtactgatc tctaacggtc
     5041 agggtaaagc ggtcgcgt
//
LOCUS       DACAPB010000256         5033 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.259, whole genome shotgun sequence.
ACCESSION   DACAPB010000256 DACAPB010000000
VERSION     DACAPB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.259"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..153
                     /locus_tag="IT904_004379"
     CDS             <1..153
                     /locus_tag="IT904_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP3194067.1"
                     /translation="ELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            322..993
                     /gene="phoP"
                     /locus_tag="IT904_004380"
     CDS             322..993
                     /gene="phoP"
                     /locus_tag="IT904_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAP3194068.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            993..2453
                     /gene="phoQ"
                     /locus_tag="IT904_004381"
     CDS             993..2453
                     /gene="phoQ"
                     /locus_tag="IT904_004381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAP3194069.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNLLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            2529..3650
                     /locus_tag="IT904_004382"
     CDS             2529..3650
                     /locus_tag="IT904_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP3194070.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(3699..4925)
                     /gene="pepT"
                     /locus_tag="IT904_004383"
     CDS             complement(3699..4925)
                     /gene="pepT"
                     /locus_tag="IT904_004383"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP3194071.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         1244 a         1259 c         1270 g         1260 t
ORIGIN      
        1 gagctgactc gcggtaagcg cgttgacgcc gaaggcatga agcagtttat cgatggtctg
       61 gcgttgccag aagaagagaa agcccgcctg aaagcgatga cgccggctaa ctatattggt
      121 cgagctatca cgatggttga tgagctgaaa taaacctcgt atcagtgccg gatggcgatg
      181 ctgtccggcc tgcttattaa gattatccgc tttttatttt ttcactttac ctcccctccc
      241 cgctggttta tttaatgttt acccccataa ccacataatc gcgttacact attttaataa
      301 ttaagacagg gagaaataaa aatgcgcgta ctggttgttg aagacaatgc gttgttacgt
      361 caccacctta aagttcagat tcaggatgct ggtcatcagg tcgatgacgc agaagatgcc
      421 aaagaagccg attattatct caatgaacat ataccggata ttgcgattgt cgatctcgga
      481 ttgccagacg aggacggtct gtcactgatt cgccgctggc gtagcaacga tgtttcactg
      541 ccgattctgg tattaaccgc ccgtgaaagc tggcaggaca aagtcgaagt attaagtgcc
      601 ggtgctgatg attatgtgac taaaccgttt catattgaag aggtgatggc gcgaatgcag
      661 gcattaatgc ggcgtaatag cggtctggct tcacaggtca tttcgctccc cccgtttcag
      721 gttgatctct ctcgccgtga attatctatt aatgacgaag tgatcaaact gaccgcgttc
      781 gaatacacca ttatggaaac gttgatacgc aataatggca aagtggtgag caaagattcg
      841 ttaatgctac aactctatcc ggatgcggag ctgcgggaaa gccataccat tgatgtactg
      901 atgggacgtc tgcgcaaaaa aattcaggca caatatccgc aagaagtgat taccaccgtt
      961 cgcggccagg gctatctgtt cgaattgcgc tgatgaaaaa attactgcgt ctttttttcc
     1021 cgctctcgct gcgggtacgt tttctgttgg caacggcagc ggtagtactg gtgctttcgc
     1081 ttgcctacgg aatggtcgcg ctgatcggtt atagcgtcag tttcgataaa actacgtttc
     1141 ggctgttacg tggcgaaagc aatctgttct atacccttgc gaagtgggaa aacaataagt
     1201 tgcatgtcga gttaccagaa aatatcgaca agcaaagccc caccatgacg ctaatttatg
     1261 atgagaacgg gcagctttta tgggcgcaac gtgacgtgcc ctggctgatg aagatgatcc
     1321 agcctgactg gctgaaatcg aatggttttc atgaaattga agcggatgtt aacgatacca
     1381 gcctcttgct gagtggagat cattcgatac agcaacagtt gcaggaagtg cgggaagatg
     1441 acgacgacgc ggagatgacc cactcggtgg cagtaaacgt ctacccggca acatcgcgga
     1501 tgccaaaatt aaccattgtg gtggtggata ccattccggt ggagctaaaa agttcctata
     1561 tggtctggag ctggtttatc tatgtgctct cagccaatct gctgttagtg atcccgctgc
     1621 tgtgggtcgc cgcctggtgg agtttacgcc ccatcgaagc cctggcaaaa gaagtccgcg
     1681 aactggaaga acataaccgc gaattgctca atccagccac aacgcgagaa ctgaccagtc
     1741 tggtacgaaa cctgaaccga ttgttaaaaa gtgaacgcga acgttacgac aaataccgta
     1801 cgacgctcac cgacctgacc catagtctga aaacgccact ggcggtgctg caaagtacgc
     1861 tgcgttctct gcgtagtgaa aagatgagcg tcagtgatgc tgaaccggta atgctggagc
     1921 aaatcagccg catttcacag caaattggct actacctgca tcgtgccagt atgcgcggcg
     1981 ggacattgct tagccgcgag ctgcatccgg tcgccccact gctggacaat ctcacctcgg
     2041 cgctgaacaa agtgtatcaa cgcaaagggg tcaatatctc tctcgatatt tcgccagaga
     2101 tcagctttgt cggtgagcag aacgattttg tcgaggtgat gggcaatctg ctggataatg
     2161 cctgtaaata ttgcctcgag tttgtcgaaa tttctgcaag gcaaaccgac gagcatctct
     2221 atattgtggt cgaggatgat ggacccggta ttccattaag caagcgagag gtcattttcg
     2281 accgtggtca acgggttgat actttacgcc ctgggcaagg tgtagggctg gcggtagccc
     2341 gcgaaatcac cgagcaatat gagggtaaaa tcgtcgccgg agagagcatg ctgggcggtg
     2401 cgcggatgga ggtgattttt ggtcgccagc attctgcgcc gaaagatgaa taaatatgtc
     2461 catacttcac gcattacgtt aagcatccgt tataatcggt tgcagatacc agcctgtgga
     2521 tgcttaacat ggaataccaa ctcactctta actggcccga ttttcttgaa cgtcactggc
     2581 agaaacgccc ggtggtgtta aaacgcggct ttaataattt tattgacccg atctctccag
     2641 acgagttggc gggtctggcg atggaaagcg aagtcgacag tcgactggtc agtcaccagg
     2701 atggcaaatg gcaggtcagc cacggtccgt tcgaaagcta cgatcatctc ggtgaaacta
     2761 actggtcatt gttagtgcaa gcagtaaatc actggcatga gccgaccgcc gcgctgatgc
     2821 gaccgttccg tgaactaccg gactggcgta ttgatgatct gatgatctct ttttctgtac
     2881 ctggcggcgg cgtcggcccg catctcgatc agtacgacgt gtttatcatt cagggtaccg
     2941 gacgtcgtcg ctggcgagtg ggtgaaaagc tgcaaatgaa acagcactgc ccacacccgg
     3001 atctgttaca ggtcgatccg ttcgaagcca tcatcgatga agagctggag cctggcgata
     3061 ttctttatat tccgccagga ttcccgcatg aaggctacgc gctggaaaat gcgatgaact
     3121 attccgtggg ttttcgcgcg ccaaatacgc gggaattaat tagcggattt gccgattatg
     3181 tgctgcaacg tgaactgggc ggcaactact acagcgatcc tgatgttcca cctcgcgctc
     3241 atcctgcgga cgttctgccg caagagatgg ataaactgcg tgagatgatg ctcgaattga
     3301 tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt tatatcccag tcacgtcatg
     3361 aactggatat cgcgccgcca gagccgcctt atcagccgga tgaaatctac gatgcgctga
     3421 aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt attgcgcatt ggcgacgacg
     3481 tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc ggcactggat gcactcgcca
     3541 gcaacattgc gctgaccgcg gagaattttg gcgatgcgct ggaagatccg tcattcctcg
     3601 cgatgctcgc ggcgctggtc aatagcggat actggttctt cgaagggtaa gtttggattt
     3661 aagccggatg cggcatcaca tgccgcatcc cttttcgctt acttccgttg cgccgttaac
     3721 tcggcaatac ggacgatcac ctgcaccgct ttttccatac cttccagagt cacaaactca
     3781 tgcttaccat gatagttgta accgccagtg aacaagttcg ggcacggtaa tcccataaac
     3841 gacaactgcg cgccgtcggt accaccgcgg atcggtttca gttccggttc aatatcgcag
     3901 tcacgcatcg cctgctgggc gatatcgaga atatgcggat gctcaaccac tttctcgcgc
     3961 atattgtagt aactgtcttc aatcaccagt tcaatgtagc aatcaggatg taaccctttg
     4021 cccacttttt tggcgatctc catcatttta cgtttacgcg cttcaaactg tttacggtcg
     4081 aaatcacgga tgatgtagtg catatcggct cggtcaacgg tgcctttcat gctcgccagg
     4141 tgatagaaac cttcatagcc ttctgtcatt tccgggcttt catccgccgg aacttccgca
     4201 tgaatacgtg ccgccagcga cagcgcattt accatcactc ctttcgccgt gcccggatga
     4261 acattgttac cgacaatttt gatattgacc gatgcggcgt tgaagttttc aaactccagt
     4321 tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg catcgaaggc atcaacatca
     4381 aaatgtttcg cccctttgcc cacttcttca tccggggtaa aggcgacgcg aatatcacca
     4441 tgcggaatgt ttttctgttg caataccgcc agcgcggtca tgatttctgc aataccggct
     4501 ttgtcatcgg cacctaacaa ggttttacca tcggtagtaa tcagcgtctg acccagtagc
     4561 tgatgcagca ccgggaacat aaccggtgat aaaacttcat cgccgatacc cagcgcaata
     4621 tcgccaccgc gatagttttc aacaatttgc ggattcacat ttttgccgct gcaatccggt
     4681 gaggtatcca catgagaaat aaagccaatc gccgggatat cgccagggac gttagccggt
     4741 aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat tgataagccc catctcttcg
     4801 agctgctctt tcagcagatg caataacttc cattggcctt ccgtgctggg aacctgtctc
     4861 acccctgctt ttgattgggt atccagagac acgtagttca aaaatcgctc aagtagttta
     4921 tccatgtagt caccctcact ttttgtgaca acattattaa gaagcaagaa aagacaaata
     4981 ttgcgtcagg tcacttttac ccctgcaagc gggaatattt atcagcatta cct
//
LOCUS       DACAPB010000257         4938 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.260, whole genome shotgun sequence.
ACCESSION   DACAPB010000257 DACAPB010000000
VERSION     DACAPB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.260"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..932)
                     /gene="tuf"
                     /locus_tag="IT904_004384"
     CDS             complement(<1..932)
                     /gene="tuf"
                     /locus_tag="IT904_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP3194072.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
     gene            complement(1047..1122)
                     /locus_tag="IT904_004385"
     tRNA            complement(1047..1122)
                     /locus_tag="IT904_004385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1087..1089),aa:Thr,seq:ggt)
     gene            complement(1129..1203)
                     /locus_tag="IT904_004386"
     tRNA            complement(1129..1203)
                     /locus_tag="IT904_004386"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Gly,seq:tcc)
     gene            complement(1320..1404)
                     /locus_tag="IT904_004387"
     tRNA            complement(1320..1404)
                     /locus_tag="IT904_004387"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1368..1370),aa:Tyr,seq:gta)
     gene            complement(1413..1488)
                     /locus_tag="IT904_004388"
     tRNA            complement(1413..1488)
                     /locus_tag="IT904_004388"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Thr,seq:tgt)
     gene            1850..2800
                     /gene="coaA"
                     /locus_tag="IT904_004389"
                     /gene_synonym="panK"
     CDS             1850..2800
                     /gene="coaA"
                     /locus_tag="IT904_004389"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP3194073.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2829..3794)
                     /gene="birA"
                     /locus_tag="IT904_004390"
     CDS             complement(2829..3794)
                     /gene="birA"
                     /locus_tag="IT904_004390"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAP3194074.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3791..4819)
                     /gene="murB"
                     /locus_tag="IT904_004391"
     CDS             complement(3791..4819)
                     /gene="murB"
                     /locus_tag="IT904_004391"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP3194075.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1255 a         1257 c         1121 g         1305 t
ORIGIN      
        1 atgtacactt cagattcgaa cttggtgtgc ggcttgatgg tgcccggctt agccagtacc
       61 tgaccacgtt cgatttcttc acgtttgata ccacgcagca gaacacctac gttctcacca
      121 gcacggcctt cgtccagcag tttgcggaac atttcaacgc cagtacaggt agacttctga
      181 gtctctttga taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca
      241 cgaccggtaa caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg
      301 aacggcttgt caatcgcacg ctctggttcc ggaatgtaag aatccaggaa gccagccagt
      361 tccaggattt tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga
      421 acgatcggag tgtcgtcgcc cgggaagtca tactgagaca gaagttcacg aacttccatt
      481 tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg
      541 atgtacggaa cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg
      601 ccgtcagtcg cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg
      661 tttttaacat agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg
      721 tcgtattcaa cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta
      781 tcgatctggt cgaatgcgcg agcagcaccg ccgtaggttt tagccaatac ggtagtgatt
      841 gcagcggtca gcgttgtttt accatggtca acgtggccga tagtaccgac gttaacgtgc
      901 ggttttgtac gttcaaactt ttctttagac atcgattgtc cctctaagac acggataaat
      961 cggtgatatc accacatcaa ccaggcaaca tgcccgactt gttgaatgca ataaacagaa
     1021 ggaaaaaaca gggaggagaa aagaagtggt gctgataggc agattcgaac tgccgacctc
     1081 acccttacca agggtgcgct ctaccaactg agctatatca gcacatcttg gagcgggcag
     1141 cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt atacgatgcc
     1201 cgcatcctgg aactcggcta cctgattttc attctgcact gaatatcgag agaagctctc
     1261 ttcattcgag ccggtaagcg aacttatcgt ctcgggctac gccatcgcgt ggccgaaatt
     1321 ggtggtgggg gaaggattcg aaccttcgaa gtctgtgacg gcagatttac agtctgctcc
     1381 ctttggccgc tcgggaaccc caccggactt gatggtgccg actaccggaa tcgaactggt
     1441 gacctactga ttacaagtca gttgctctac ctactgagct aagtcggcat caagtagcgc
     1501 gcattctatg gagacatgcg agttcatgca actaaaaaat tgcataattt gttttattgg
     1561 tcacatttta tgcgacacga tgaagaaaca gccgaacgga gggcagatta gcacactttt
     1621 tcaacatcat tgtgctcaac aatgcgctcc tgctaaacca taattctttt tatcagatgg
     1681 aatatctgtc acattgcttt tcaacgatag cttcctggca gagatttttt cttattattc
     1741 ctccccatct ggtgttaccc tcctgcccat taacccattc aacagaactg tgacgcgcca
     1801 tggcaaatat cgctttgccg atagagctat gaccgccaga aacatgctta tgagtataaa
     1861 agagcaaacg ttaatgacgc cttacctaca gtttgaccgc aaccagtggg cagctctgcg
     1921 tgattccgta cctatgacgt tatcggaaga tgagatcgcc cgtctcaaag gtattaatga
     1981 agatctctcg ttagaagaag ttgccgagat ctatttacct ttgtcacgtt tgctgaactt
     2041 ctatataagc tcgaatctgc gccgtcaggc agttctggaa cagtttcttg gtactaacgg
     2101 gcaacgcatt ccttacatta tcagtattgc tggcagtgtc gcggtgggga aaagtacaac
     2161 cgcccgtgta ttgcaggcgc tattaagccg ttggccggaa catcgtcgtg ttgaactgat
     2221 cactacagat ggcttccttc accctaatca ggttctgaaa gaacgtggtc tgatgaagaa
     2281 gaaaggcttc ccggaatcgt atgatatgca tcgcctggtg aagtttgttt ccgatctcaa
     2341 atccggcgtg ccaaacgtta cagcccctgt ttactcgcat cttatttatg atgtgatccc
     2401 ggatggagat aaaacggttg ttcaacctga tattttaatt cttgaagggt taaatgtctt
     2461 acagagcggg atggattatc cacacgatcc acatcatgta tttgtttctg attttgtcga
     2521 tttttcgata tatgttgatg caccggaaga cttacttcag acgtggtata tcaaccgttt
     2581 tctgaaattc cgcgaagggg cttttaccga cccggattcc tattttcata actacgcgaa
     2641 attaactaaa gaagaagcga ttaagactgc catgacattg tggaaagaga tcaactggct
     2701 gaacttaaag caaaatattc tacctactcg tgagcgcgcc agtttaatcc tgacgaaaag
     2761 tgctaatcat gcggtagaag aggtcagact acgcaaataa tttgcagggg agcgaatact
     2821 cccctttctt atttttctgc actacgcagg gatatttcac cgcccatcca gggttttatt
     2881 attccatcct gctcaagtaa taaagccccc tgtttgtcta ttccgcgtga aatgccaaat
     2941 atttctttat caccaatgat aagtttcact gggcgattaa taaaattatc cagcttttcc
     3001 cagcgcgaca gataaggtgc caatccttct tgttcgaaga gttccaacgc agcacgtaat
     3061 tcacgtatta gcatggccgc caacgtatta cgatcgagat tgatccccgc ttcctgcagc
     3121 gtgatccacc cctgattaac gacactctct tcaacacggc gcattgccat gttgatcccg
     3181 gctccaatga ctatttgcgc cgcatcgcca gttttgccag tcagctccac aagaatgcct
     3241 gccagcttgc gatcctgcag atagaggtca ttaggccatt tgacacgaac tttatctgct
     3301 cccagcttgc gtaatacttc cgccatcacg ataccgataa ccagacttaa accaatcgcc
     3361 gccgccgggc cttgttccag acgccagaac atcgacaaat ataagtttgc gccaaaaggc
     3421 gaaaaccatt tccgcccccg gcgaccacgg ccagcctgct ggtattctgc aacacaggca
     3481 tcgcccgatt taagctctcc gatacgatca agaaggtact gattcgtgga gtcaataact
     3541 ggcagcacgg ctacactacc gccatccagc tgacccaata tctgttcagc attaagtaac
     3601 tggatgggct caggcaggct gtatccttta cccggaacgg taaagacatc aacgccccag
     3661 tcacgcagtg tctgaatgtg tttattaata gccgcccggc tcattcccag cgtttcaccc
     3721 aactgctcgc cagagtgaaa ttcaccgttc gctaacaggg caatcaattt cagtggcacg
     3781 gtgttatcct tcatgaaatt gtctccactg cgctcacttc acctgatgca ccaataaagc
     3841 ggacttcagg ctcaagccag acattaaatt tttcacccac tttttgtctt acatggtgtg
     3901 ccagttgcac cacatcttcg ctttttgcat tgtcttcatt aatgagaact aacgcctgtt
     3961 gacggtgcac cgcagcccca cccatttgca tcccttttag ctggcactga tcaataagcc
     4021 aacctgctgc cagttttact gaaccatccg cctgggggta atttggtgct gttggaaatt
     4081 gtgacagtaa tgcttcagcc gtttcggcag atacaacagg gtttttgaag aaactaccgg
     4141 cattgccatt cacttttgga tcagggagtt tggtggtgcg catatgacac accgcattaa
     4201 atacttgctg tggcgttact gttgtaggat ccagacgagt taagtcacca tacgttagta
     4261 caggttgcca ctcttttggc agacgcagac ctacggctac aatggcgaag cgatcctggt
     4321 attcatgttt aaaaatactg tcgcgatagc caaaacggca ctctttggca gttaagcgca
     4381 cttgcttgcc tgtcgccagt tcaacacaat caacataagc gcaaactcgc tgtaattcta
     4441 cgccataagc accaatattc tggataggtg atgagccgac acaaccagga attaatgcca
     4501 gattttccag accaggcata ccttcctgca aagtgtattt taccaggcga tgccagtttt
     4561 ctccggctcc tacatgtaaa taccacgcat caggttcatc atgaatttcg atacctttga
     4621 tccggttgat gatcaccgtg ccgcgatagt cttccagaaa aagtacatta cttccttcac
     4681 ccagaataag aacgggttgt ccttctgcgg ttgcatgctg ccaggcattg agtaattgtt
     4741 gttcgtcttc ggcacataca atgtgctgag cattatgatc aatgccaaat gtgttccaag
     4801 gttttaagga gtggttcata gctgctttcc tgatgcaaaa acgaggctag tttaccgtat
     4861 ctgtgggggg atggcttgta gatatgacga caggaagagt ttgtagaaac gcaaaaaggc
     4921 catccgtcag gatggcct
//
LOCUS       DACAPB010000258         4833 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.262, whole genome shotgun sequence.
ACCESSION   DACAPB010000258 DACAPB010000000
VERSION     DACAPB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.262"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(93..743)
                     /gene="rcsB"
                     /locus_tag="IT904_004392"
     CDS             complement(93..743)
                     /gene="rcsB"
                     /locus_tag="IT904_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAP3194076.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(760..3432)
                     /gene="rcsD"
                     /locus_tag="IT904_004393"
     CDS             complement(760..3432)
                     /gene="rcsD"
                     /locus_tag="IT904_004393"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAP3194077.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            4169..>4833
                     /locus_tag="IT904_004394"
     CDS             4169..>4833
                     /locus_tag="IT904_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAP3194078.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRT"
BASE COUNT         1240 a         1134 c         1155 g         1304 t
ORIGIN      
        1 atcgtaggcc ggataaggcg tttacgctgc atccggcaat cggtgcaaat gccagataag
       61 acactaacgc gtcttatctg gcctacagat gattagtctt tatctgccgg acttaaggtc
      121 acagaagaga gataattcag cagggcgata tcgttctcga cacccagctt catcatcgca
      181 gatttcttct ggctactgat ggttttaata ctgcggttca gctttttagc gatctcggtc
      241 accaggaagc cttccgcaaa caggcgcaga acttcactct cttttggcga gagacgcttg
      301 tcaccgtaac caccagcact gattttttcc aacaggcgag aaacgctttc cggggtaaat
      361 ttcttccctt tctgcagcgc ggcgagagct ttcggcagat cggtcggtgc accttgtttc
      421 agcacgatcc cttcgatatc cagatccaat accgcactaa gaatcgccgg gttgttgttc
      481 atggtcagaa caataatcga caggcttggg aaatggcgct tgatgtactt gattaaggta
      541 atgccatcgc cgtacttatc gccaggcatg gagagatcgg taatcaacac atgcgcatcc
      601 agtttcggca ggttgttgat cagtgctgta gagtcttcaa attcgccgac aacattcacc
      661 cactcaattt gctcaagtga tttgcgaata ccgaacaaga ctatcggatg gtcatcggca
      721 ataattacgt tcatattgtt catgtaatag gctaccttgc tacagcaagc tcttgacata
      781 actgtcaatg tcgctgatgt atttttctat tcctggaaca tccttctcac gaatcagatg
      841 ttccagcgtt tcacataact gcttgccggg taccagattt agcatggcaa atacgccttt
      901 aagacgatga gccgtttggg ctaacgcagc aaagtcactg gttgctgctt cagtatacag
      961 cctcttaaca tcatccggta ctgtgtctac aaagagcgca taatagccgc tggcatggag
     1021 ttgcgcattt tcatctccgc ccagaggcga ttctgtcacc tcttcctgcg ccagttgcac
     1081 ttcaattaat tgtaagaccg cttcctgcat agcgttgctc atattgaagt tgacgcacaa
     1141 ttgaccaggc ccaatttccc gtacgccaga ctcatcatcg cttaaaagca agccagaggc
     1201 agtaagatta gacggattat ccgttaaaaa gatatcataa tcttgactaa ttaatctttc
     1261 atcgggtgtg atacaggttg caccccaatt ttctaactgg cgagtgacaa tattccgaat
     1321 ttctgccgaa gtaacatcca ccattacgca gacatcatcc agtaaacgct cttcttcctc
     1381 ttcaacttcc gggtcagctg cgagcatttt gatatgcaca gagtagcgtg taccaagccc
     1441 atcccgcgtt ttgatgttta aatgaccgcc cagtttacgt gccagttgat cgctcagcca
     1501 gaatgccagc gggtccgcct tgccatagcg atcgttttgg gtctggttga taaacgggaa
     1561 gtgcaaatta tccatttcat gaatgcttac gccttctccg gtgtccagaa tgcggaacgt
     1621 caggcggtct tcggaggact catcctgatc aacctcaagg gtgatttttc ccaattgcgt
     1681 tgaggtcacg gcatattgca tcagtagcag caaaatacgt cgtaaggcat cgcgatcgcc
     1741 gcggcgcata tcgtgtgctt tcagatgatt gttaatcagc agttgcagac ctttacgctt
     1801 gatggcaggc aacactgaag gcacaacttc atcaattaaa tcctgcacgg agaacagcac
     1861 cgtctcactt ttccagctat cgtccgcaag catgttcgct aactgtattt catcgaccag
     1921 ccgcaccagc acatctgcct gattcgccag ttgtttgctt tccggggcat tgagtttagc
     1981 cgcgctctcc gccagggact gtgcgggttc tttcagcgca tcgccaatgt ttttcataaa
     2041 ggtcatccgc ccctgctggt ttttctcata cagacgctgc gcctgcttga gtttcttgtt
     2101 taccagcact tcgcgatcct gatcgcgaat aatgaaaatt tgtgtgcgcg gcgcgacctg
     2161 gctgcggaac atgcggatct catacagctc gttattgatc gtcgcctgaa taatcccctg
     2221 atgctgttcc gccatggtgg tgatgttttg cagattcaaa tgcggcagca aatgatcggc
     2281 aattttgtta cttatgacag tgcggttcga ttcctgatcg tgaaccagca ggccgagcgg
     2341 cagcagtgag actatctctt cattgattgc ccgtaaaatg cgcaattcgt tattgaccgc
     2401 cgtgttgggt agactttctg tactgcgact ggagaaatgg cggaatgtgg tatagccaaa
     2461 taacgccagc gccagcaaac cgatgttcag cagcagtggc agcagaatgt tttgcaacgt
     2521 atccagcaat aaggtgccat aaggaacctg ccagaccagg cgcatatcgg tagagttgag
     2581 tgccgaggag atttcaatct tcgtactgtt aaagtggata ctgacgctat ccgtcccttc
     2641 tttctcatta tcattgtctc ccgttgccgt cgcgtctggc tcaaggcgga aactgtccag
     2701 cggcataccc ggtgggatca aatcattaat cggcagatca aaagccacga ccgttgccag
     2761 atgtcccggc tggttgaacg tggtacgcaa ggtaaagtaa tgaccgttct gccaggccag
     2821 gcggcgcatg ttagaaaaac tttcgcgttc atcgagggcg ttggcctgtt gcaacatttc
     2881 tgcacgacgt gaatcaacaa tgtcactgac ggtcgattct ttaaatccgg aggtgagatc
     2941 tttgaggggc agggttgaga tcagtaccag actgttatcc tgaccattca ggtaatacat
     3001 cgaccacggt acattttctg caccccacaa tgtatccaga taagtggaca tccgctgagt
     3061 catctcaagc gttgagttgt cgtgagagcc aaagattaac gcttccgttt tgcggcgtgg
     3121 tttctccaga tagtagacat cctgtttcag gcgcgtctct tgtaaacctt cgccggagga
     3181 gggggaggtc gtcgcggcaa tgttgtcgta gatctgccag gtcacgtaac gccaggtatc
     3241 gacgcgcttt tggattgcgt gggtaatgtc gacaatctgg taacttttat ctttcagcca
     3301 ggcgttaacg gcgctttgta ccattacccc catcgtcacc agtaacacaa tgatcaacag
     3361 taaaaagaag cgggtaatgc tccccggtag gagtgaaaag cgggtcgtgg ccgttgtctc
     3421 tttctgacgc attcgtgttt atgacctgtt aaaacttcgc gaagtgatag ggcagtataa
     3481 agggtacagt gtgaaattcc agactcttac ctgattgatg attctctatt ctcccgtgat
     3541 atttatcatc ctgcattggg gaaatagatg tacaagcgcc attttgctgt acaaataacc
     3601 tacaaaaagc ccaacgaaat taattgcaaa taaacgaaga ttgctggaaa ttatgctgat
     3661 gttaattatt tgtgaaatag ttaacaagag ttatagtttt tctgtggtag cacagaataa
     3721 tgaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt gaaatagggg
     3781 taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga tagcgggaga
     3841 tattctagtt gcgagtgaag gttttgtttt gacattcagt gctgtcaaat acttaagaat
     3901 aagttattga ttttaacctt gaattattac tgcttgatgt taggtgctta tttagccatt
     3961 ccgcaataat cttaaaaagt tcccttgcat ttacattttg aaacatctat agcgataaat
     4021 gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag
     4081 aatggacttg ccgactgatt aatgagggtt aatcagtatg cagtggcata aaaaagcaaa
     4141 taaaggcata taacagaggg ttaataacat gaaagttaaa gtactgtccc tcctggtccc
     4201 agctctgctg gtagcaggcg cagcaaacgc tgctgaagtt tacaacaaag acggcaacaa
     4261 attagatctg tacggtaaag tagacggcct gcactatttc tctgacaaca agtcagaaga
     4321 cggcgaccag acctatgtac gtcttggttt caaaggcgaa actcaggtta ctgaccagct
     4381 gaccggttac ggccagtggg aatatcagat ccagggcaat acctctgaag acaacaaaga
     4441 aaactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtag gttctttcga
     4501 ctacggtcgt aactacggcg ttgtttacga cgtaacttcc tggaccgacg tactgccaga
     4561 attcggtggc gacacctacg gttctgacaa cttcatgcag cagcgtggta acggcttcgc
     4621 gacctaccgt aacaccgact tcttcggtct ggttgacggt ctgaactttg ctgttcagta
     4681 ccagggcaaa aacggcagcg taagcggcga aggcatgacc aacaacggtc gtggcgctct
     4741 gcgtcagaac ggcgacggtg tcggcggttc tatcacttat gattacgaag gcttcggtat
     4801 cggtgctgca gtttccagct ccaaacgtac tga
//
LOCUS       DACAPB010000259         4807 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.263, whole genome shotgun sequence.
ACCESSION   DACAPB010000259 DACAPB010000000
VERSION     DACAPB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.263"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..395
                     /locus_tag="IT904_004395"
     CDS             <1..395
                     /locus_tag="IT904_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3194079.1"
                     /translation="KTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMTYEKVEIIRRN
                     DGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVTPFSYANTQFKNVPSTFQVGYIN
                     DFGGLSFYEINCPVVNNICNISVANRDQ"
     gene            397..1461
                     /locus_tag="IT904_004396"
     CDS             397..1461
                     /locus_tag="IT904_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP3194080.1"
                     /translation="MRYLLIVITFFMGFSSLPAWAMDCYAEHEGGNTVVIGYVPRISI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPGPSPGEEYPSAWANIMMLLASGQDFYNQNSY
                     TFGVTYNGVDYDSTSPLPIAAPVCIDIKGAGTFGNGYKKPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(1504..1704)
                     /gene="glgS"
                     /locus_tag="IT904_004397"
     CDS             complement(1504..1704)
                     /gene="glgS"
                     /locus_tag="IT904_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAP3194081.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            1973..2602
                     /locus_tag="IT904_004398"
     CDS             1973..2602
                     /locus_tag="IT904_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAP3194082.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALLYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            2629..4290
                     /locus_tag="IT904_004399"
     CDS             2629..4290
                     /locus_tag="IT904_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP3194083.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDASTGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLTALTSPLTSTTPVAENVE"
     gene            4529..4588
                     /locus_tag="IT904_004400"
     CDS             4529..4588
                     /locus_tag="IT904_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP3194084.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT         1399 a         1038 c         1093 g         1277 t
ORIGIN      
        1 ccaaaacaga taaaaaaaat gtgatgcaag tcactatgca gcacgcattg aaactattct
       61 ggcggccaaa agccattgaa ttagaagacg atggggtaat gacttacgaa aaagttgaaa
      121 tcattcgtag aaatgacggt tcgatccgct ttaacaataa gatgccttat cacgttacgc
      181 tgggttatat cggaacaaat ggggtaacga tgttaccaca aacgcaaagc ttaatggtaa
      241 ccccttttag ctatgcaaat acgcagttta aaaatgttcc gtcaactttt caggtgggtt
      301 atatcaatga ctttggtgga ttaagttttt atgaaataaa ctgccctgta gtaaataaca
      361 tttgtaatat ttctgtagcc aaccgagatc aataatatgc gctacttgtt aattgttatt
      421 acttttttta tgggttttag ttcattacct gcatgggcaa tggactgcta tgctgaacat
      481 gaaggtggaa ataccgttgt cattggttac gtaccaagaa tctctatccc cagcgatggt
      541 aaaaaaggtg ataaaatctg gcaaagcagt gaatatttta tgaatgtttt ctgtaataat
      601 gcactaccag gcccatctcc aggagaagaa tacccatctg catgggcaaa tataatgatg
      661 ttgttagcat caggtcaaga cttttataat caaaactctt atactttcgg tgtaacttat
      721 aatggggttg attatgattc tacatctcca ctacctattg cagcacctgt atgtattgat
      781 ataaaaggag ccggaacctt cgggaatggc tacaaaaagc ccgcagtctg tagtggcggt
      841 cctgaaccgc aattatcagt aacttttcca gtacgagtgc agctatatat taagctagct
      901 aaaaatgcca ataaagtaaa taaaaaactt gtattacctg acgaatatat agcacttgaa
      961 tttaaaggta tgagcggagc aggtgctata gaagtagata aaaatctgac cttcaggatt
     1021 cgcggattaa ataacattca tgtccttgac tgctttgtta atgttgatct ggaaccagct
     1081 gatggcgttg tcgactttgg taaaataaat tcccgaacaa ttaaaaatac cagcgtgagt
     1141 gagacgttta gcgtagtcat gaccaaagat ccgggtgcgg cctgtactga gcagtttaat
     1201 attttaggga gttttttcac tacggatatt ttgagtgatt atagccatct ggatataggt
     1261 aatggtctgc tattgaagat atttcataac gatggaacag caacggaatt taaccgcttc
     1321 tcacaatttg cttctttttc atcgtctagt gcgccttcgg tcaccgcacc attcagggca
     1381 gaactgagtg cgaacccggc agaaacggtt gttgagggac cgtttagtaa agacgtaatc
     1441 ctgaaaatca cctataacta gtatctaata caaacactaa aacgggccat caggcccgtt
     1501 atatcagtgc tctaactcca gctctcttgc ctgcatcatc tgttgacaat accaggcata
     1561 acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg ccagaatatc
     1621 gaccgggcga ccttctgcgt gcattctggc aaaactacgc gccaggaaat cgaaattatt
     1681 taaagaatta agactatgat ccataagcac atcctccggt tatcgcatac cttttaaggc
     1741 catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcctttaa acataacgtg
     1801 cgtaaatata ttgtccccga tcacactttt tagtgaaaaa taaaaaacca tattaaaaga
     1861 ctttctcttc cagaacttct ctaacctgtg ttattattaa cacagataaa tgtaagcaag
     1921 gaaactatta caaagtaaat attgcgtcac taaatggaca ttggagtgat atatgatttt
     1981 attcgccgac tataacaccc cttatctttt cgctatttcc tttgtactgt tgatcggcct
     2041 gttagaaatt tttgcgctta tctgcggtca catgctctcc ggtgctcttg atgcacatct
     2101 tgaccattat gattccataa ccacaggtca tattagccag gcacttcttt atctcaatat
     2161 tggaagattg ccagctctcg tcgttctctg cttactggct ggtttttttg gtcttattgg
     2221 catcctgttg cagcatgcct gcatcatggt ctggcaatca ccactgtcga acctgttcgt
     2281 ggttcctgtc agtttactgt ttacgattat tgcggtgcat tataccggta aaatagttgc
     2341 gccctggatt ccacgcgacc atagttcagc tattacagaa gaagaatata ttggtagcat
     2401 ggcgttaatt accggtcatc aagccacttc aggtaatccc tgcgaaggaa aactcaccga
     2461 tcaatttggc caaatacatt atttactact ggagcctgaa gaaggaaaaa ttttcactaa
     2521 aggagacaaa gtgttaatta tttgccgact ttctgcaacg cgatatttgg cggaaaacaa
     2581 tccctggccc caaatattgt aatttattaa tcaaaaggaa atttaataat ggatgatatt
     2641 gttaattctg tgccctcctg gatgtttacc gcgattattg ccgtatgcat tctgtttatt
     2701 attggaatta ttttcgccag gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt
     2761 actggtttag gtgggcaaaa agtggtaatg agcggtggcg caatcgtgat gccgatcttt
     2821 catgaaataa tccccatcaa tatgaatact ctgaagctgg aagtcagccg ctcaaccatt
     2881 gatagcctga ttacgaaaga tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg
     2941 gtaaaacctt cagtagaagg gattgccacc gctgcccaga cgctggggca acgcaccctg
     3001 tcgcctgaag acttacgtat gttggttgaa gataaatttg tcgatgccct ccgtgcaaca
     3061 gctgcgcaaa tgaccatgca tgagttacag gatacccgcg agaacttcgt gcagggggtg
     3121 caaaatacag tggcagaaga cctgtcgaaa aacggtctgg aactggagag cgtttcactt
     3181 accaacttta accagacctc gaaagaacat ttcaatccga acaatgcctt tgacgccgaa
     3241 ggtttaacca aactgactca ggaaacagag cgccgtcgcc gcgaacgtaa cgaagttgaa
     3301 caggatgtag aagttgcggt gcgtgaaaaa aatcgcgatg cgctatcgcg caagctggag
     3361 attgaacagc aagaagcgtt tatgacgctt gagcaggagc agcaggttaa aacccgtact
     3421 gccgaacaga atgcacgtat tgcggctttt gaagctgaac gtcgtcgtga agcagagcag
     3481 acacgaattc tggctgaacg gcagattcag gaaacagaaa tcgaccgcga acaggccgtc
     3541 cgctcaagaa aggttgaagc tgaacgtgaa gttcgcatta aagagatcga acagcagcag
     3601 gtcaccgaaa tcgctaacca gacgaaatcg atcgctattg ccgccaaatc ggaacaacag
     3661 tcccaggcag aagcgcgtgc taatctcgca cttgcagaag cggtaagcgc ccaacaaaac
     3721 gtagaaacca ctcgccagac tgccgaagcc gatcgtgcta aacaagttgc cctaatcgct
     3781 gccgcgcagg atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa
     3841 gaagccgcag agatgcaggc ggcggctatc gttgagttag ccgaagctac acgtaaaaag
     3901 ggtctggcgg aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgat
     3961 gaacaaacca gccttaaatt caaactggcc ttgttgcagg cgctgcctgc ggtaatagag
     4021 aaatccgttg agccgatgaa gtcaatcgac ggtatcaaga ttattcaggt cgatggtctg
     4081 aatcgtggcg gcgctgcggg tgatgcgagt acaggtagcg ttagtggagg aaaccttgca
     4141 gagcaggcat tgtctgccgc cctttcttac cgcacacagg caccgctgat tgactccttg
     4201 ctcaatgaaa ttggtgtttc aggcggctca ctgacggcat tgacttcacc cttaacctca
     4261 acaactcccg tcgccgaaaa cgtagaataa attttcagct atgaacgtcc ctggcacaaa
     4321 aagccgggga ttttttatat ctgcgttccg ctaaaaagtg caaatgatca ggttgttgca
     4381 gcattttgcg tgacggctcg gggaaggcaa aactgcatca gagaaagcat tgcgtggggt
     4441 ctggcgctca tcaacaatcg gggagtcagc agggggctga aacgggaaag cccctcccga
     4501 agaaggggcc ttacagaagg aaagggttat gatgaagctc gtcatcatac tgattgtgtt
     4561 gttactcgta agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc
     4621 acccttgttc ctttacttta ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac
     4681 atatccctgg cccaaaaagc cggggatttt ttatatctgc gttccgctaa aaggtgcaaa
     4741 tgctcaggac gttgcagcgt tttgcgtgac cgctcgggga aggcaaaatt gcctctggga
     4801 aagcatt
//
LOCUS       DACAPB010000260         4732 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.264, whole genome shotgun sequence.
ACCESSION   DACAPB010000260 DACAPB010000000
VERSION     DACAPB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.264"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..160)
                     /locus_tag="IT904_004401"
     CDS             complement(<1..160)
                     /locus_tag="IT904_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3194085.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSIN"
     gene            complement(453..734)
                     /locus_tag="IT904_004402"
     CDS             complement(453..734)
                     /locus_tag="IT904_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAP3194086.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(997..2106)
                     /gene="apbC"
                     /locus_tag="IT904_004403"
     CDS             complement(997..2106)
                     /gene="apbC"
                     /locus_tag="IT904_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAP3194087.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            2238..4271
                     /gene="metG"
                     /locus_tag="IT904_004404"
     CDS             2238..4271
                     /gene="metG"
                     /locus_tag="IT904_004404"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP3194088.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLANELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4412..>4732
                     /locus_tag="IT904_004405"
     CDS             4412..>4732
                     /locus_tag="IT904_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAP3194089.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDK"
BASE COUNT         1258 a         1162 c         1128 g         1184 t
ORIGIN      
        1 tattaatact tactgaatca ccacccgctt cgaatttaca cggagattct gcaatattcc
       61 ccttaatagt taatgttccg gtatcaacgt ctgcagcaaa tactccagcg ctcataaaaa
      121 aagaagaaaa gacagcggca gcaataattg aacgtttcat aatatgttcc ttaataaaat
      181 ttatttttgg ttgttaagag tctgtatctc ttggaaacaa atttctcaca tcaaaaaagt
      241 tgccgcaatg taaacacatt attttattga tttaaaacaa attaaccatt gcaatataaa
      301 tacaacaaat atttaagagt attaactatt tatcgcatct atcaattaat gtagatttat
      361 gtaaatggta ttgttgatat caataaaaaa gccgaaatca tttatataaa tgatttcggc
      421 tttctaaatg caattaaaat caggttgcca aattaatcgt agcgatttaa cacgattcga
      481 cgtttgctat cgctacctgc aacaaccact tctgtcgtta cacctttgac gggcgaagaa
      541 aacagatgac gggtgtcaac attatacgtt aactgaccac aatgctgttg aggttgacca
      601 ttaaccatag ccatactgca tgttggggga ctgatcctgc cattaatcgc taatgtggca
      661 gtctctgaag agaatgcggg agaagccaat ccataggcaa gaaaaataat tcctgacagc
      721 caatacttat tcattgaacg ttatccctgt agtaaaggtt atgcctggat agaatgagtg
      781 cataacaaac tatagctgta catccactac acagccacga aggatgataa tgaagcattg
      841 cctgtatgat caatcgactt tgtagagttt cggacgaagg tccgcagaat attcgcagta
      901 ttaaataagt gttcattaat gcggcaaaaa ctaatacacc gcatcaatgt aacatctctg
      961 cggtgtatga agaataacca gaatggttaa taggcgttag accgcgcgga aggaaatctc
     1021 gcctggaatg acttcaccct gccagtagag ctgagctgca acgcggtcag ccagctggcg
     1081 gtagatagcg gtaaattcgc tctccggacg gctgataacc gttggcgtac ctttatcgag
     1141 atcctcacgt aaggagatgt gtaacggcat ctgccccagc agctgtgtat ggtatttttc
     1201 agccagtttc tctgcaccac cggtaccgaa aatcggttca tggtgaccac agttactgca
     1261 aatatgcaca ctcatgttct cgacaatacc gagcaccgga acctcgactt tctcgaacat
     1321 cacaatgcct tttttcgcat cgatcagcgc gatgtcctgc ggggtggtga ccacgactgc
     1381 accggttact ggaatgtttt gcgccagcgt caattgaatg tcaccggtgc ctggcggcat
     1441 atcaagcacc agatagtcga gatccggcca caaggtttcc tgcaacatct gcattaacgc
     1501 cttgctcgcc atcggtccac gccacaccat cgcattgtcg tcagtgacca gataaccaat
     1561 tgaattggtt gccaggccat gagacataat cggtgccata tgggtaccgt ccggcgaagt
     1621 tggacgctga ttttctgcgc ccaacattgt tgggattgat gggccgtaga tatcggcgtc
     1681 cagaatgccg actttcgccc cttctgccgc cagcgccagt gccaggttta ccgccgtgga
     1741 cgatttaccc acgccgcctt taccggaact caccgcgata atgttcttca caccgttaat
     1801 tcccggctga ttcttcacgc gtttcagagt ggctatgtta tgcgacagtt tccagtcgat
     1861 agctttcgcc ccggtgatgc gcagcagttc ggcgctacat tgctctttta actcttcgaa
     1921 cgcgctatgc cagacaaacg gcataaccag ttccacatgc aacgtgtcgt ccatccaggc
     1981 gacatggtgc aacgctttca gcgtggtgag gttatgcttc agggttgggt gctgaaaatt
     2041 ggccagtgtc ccggcgacca ttgcgcgcaa ggcttctggt gatttggcct gggattgttc
     2101 gttcatcccg actccttttt tgtatagata aaccatcagc tgatagttta cctgaagaat
     2161 atagagaagt acttacttaa cattttccca tttggtacta tctaacccct tttcactatt
     2221 aagaagtaat gcctactatg actcaagtcg cgaagaaaat tctggtgacg tgcgcactgc
     2281 cgtacgctaa cggctcaatc cacctcggcc atatgctgga gcacatccag gctgatgtct
     2341 gggtccgtta ccagcgaatg cgcggccacg aggtcaactt catctgcgcc gacgatgccc
     2401 acggtacacc gatcatgctg aaagctcagc agcttggtat caccccggag cagatgattg
     2461 gcgaaatgag tcaggagcat cagactgatt tcgcaggctt taacatcagc tatgacaact
     2521 atcactcgac gcacagcgaa gagaaccgcc agttgtcaga acttatctac tctcgcctga
     2581 aagagaatgg ttttattaaa aaccgcacca tctctcagct gtacgatccg gaaaaaggca
     2641 tgttcctgcc ggaccgtttt gtgaaaggca cctgcccgaa atgtaaatcc ccggatcaat
     2701 acggcgataa ctgcgaagtc tgcggcgcga cctacagccc gactgaattg atcgagccga
     2761 aatcggtggt ttctggcgct acgccggtaa tgcgtgattc tgaacacttc ttctttgatc
     2821 tgccctcttt cagcgaaatg ttgcaggcat ggacccgcag cggtgcgttg caggagcagg
     2881 tggcaaataa aatgcaggag tggtttgaat ccggcctgca acagtgggat atctcccgcg
     2941 acgcgccata ctttggtttt gaaattccga acgcgccggg caaatatttc tacgtctggc
     3001 tggacgcacc gattggctac atgggttctt tcaagaatct gtgcgacaag cgcggcgaca
     3061 gcgtaagctt cgatgaatac tggaagaaag actccaccgc cgagctgtac cacttcatcg
     3121 gtaaagatat cgtttacttc cacagcctgt tctggcctgc catgctggaa ggcagcaact
     3181 tccgcaagcc gaccaacctg tttgttcatg gctatgtgac ggtgaacggc gcgaagatgt
     3241 ccaagtctcg cggcaccttt attaaagcca gcacctggct gaatcatttt gacgctgaca
     3301 gcctgcgtta ctactacact gcgaaactct cttcgcgcat tgatgatatc gatctcaacc
     3361 tggaggattt cgttcagcgc gtgaatgccg atatcgttaa caaagtggtt aacctggcct
     3421 cccgtaacgc gggctttatc aacaaacgtt ttgacggcgt gctggcaaac gaactggctg
     3481 acccgcagtt gtacaaaacc ttcactgatg ccgctgaagt gattggtgaa gcgtgggaaa
     3541 gccgtgaatt tggtaaagct gtgcgcgaaa tcatggcgct ggctgatctg gctaaccgct
     3601 atgtcgatga acaggctccg tgggtagtcg caaaacagga aggtcgcgat gctgacctgc
     3661 aggcgatttg ctcaatgggc atcaacctgt tccgcgtgct gatgacttac ctgaagccgg
     3721 tactgccgaa actgaccgag cgtgcagaag ccttcctcaa tacggaactg acctgggatg
     3781 gtatccagca accgctgctg ggccacaaag tgaatccgtt caaggcgctg tataaccgca
     3841 tcgatatgaa acaggttgaa gctctggtgg aagcctctaa agaagaagtg aaagcagctg
     3901 ccgcgccagt tactggccca ctggcagatg atccgattca ggaaaccatc acctttgacg
     3961 acttcgccaa agttgacctg cgcgtagcgc tgatcgaaaa cgcagagttt gttgaaggtt
     4021 ctgacaaact gctgcgcctg acactggatc tcggcggtga aaaacgcaat gtcttctccg
     4081 gtattcgttc tgcctacccg gatccgcagg cactgattgg tcgtcacacc attatggtgg
     4141 ctaacctggc accacgtaaa atgcgctttg gaatctccga aggcatggtg atggctgccg
     4201 gtccaggcgg gaaagatatc ttcctgctaa gcccggatgc tggtgctaaa ccgggtcatc
     4261 aggtgaaata atcccccttc aaggcgctgc atcgacagcg ccttttcttt ataaattcct
     4321 aaagttgttt tcttgcgatt ttgtctctct ctaacccgca taaatactgg tagcatctgc
     4381 attcaactgg ataaaattac agggatgcag aatgagacac tttatctatc aggacgaaaa
     4441 atcacataaa ttctgggcgg tggagcaaca gggaaacgag ttgcatatca gttggggaaa
     4501 agttggcacc aaagggcaaa gtcagataaa aagtttttca gatgctgcgg cagcggaaaa
     4561 agcggaactt aagctgattg cggagaaggt gaagaagggg tatgtggagc aagcgaagga
     4621 taattctttg caaccttccc aaacggtaac gggctctctc aaggtagcgg acttatccac
     4681 cattattcag gaacaaccct ctttcgtagc agaaacccgt gcgcctgaca aa
//
LOCUS       DACAPB010000261         4701 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.265, whole genome shotgun sequence.
ACCESSION   DACAPB010000261 DACAPB010000000
VERSION     DACAPB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.265"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1148
                     /gene="chbC"
                     /locus_tag="IT904_004406"
     CDS             <1..1148
                     /gene="chbC"
                     /locus_tag="IT904_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAP3194090.1"
                     /translation="TIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKVDAL
                     AAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRNWVI
                     KLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASLGS
                     VVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAAGKT
                     FHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPILF
                     GLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTNGS
                     VAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1199..1549
                     /gene="chbA"
                     /locus_tag="IT904_004407"
     CDS             1199..1549
                     /gene="chbA"
                     /locus_tag="IT904_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAP3194091.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1560..2399
                     /gene="chbR"
                     /locus_tag="IT904_004408"
     CDS             1560..2399
                     /gene="chbR"
                     /locus_tag="IT904_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAP3194092.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            2504..3856
                     /locus_tag="IT904_004409"
     CDS             2504..3856
                     /locus_tag="IT904_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP3194093.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            3869..4618
                     /gene="chbG"
                     /locus_tag="IT904_004410"
     CDS             3869..4618
                     /gene="chbG"
                     /locus_tag="IT904_004410"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAP3194094.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEQGIALRADRQMAFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDAGHRGDRSLEVMCHPAFIDNTIRQSAYCF
                     PRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
BASE COUNT         1178 a          996 c         1258 g         1269 t
ORIGIN      
        1 caaccattga aacacttaat ggtctgaaag gtattggcgg caacgtatat aacggaacat
       61 taggaataat gtctttaatg gcaccgttct ttattggcat ggcgctggca gaagagcgta
      121 aagtcgatgc gctggcggct gggttgttat ccgttgcagc atttatgacc gtcaccccat
      181 atagtgtcgg tgaggcctat gcggttggtg caaactggtt aggtggggcg aatatcatct
      241 ccgggattat tattggcctg gtggtggcag aaatgtttac ctttattgtc cgccgcaatt
      301 gggtcattaa actgcccgac agcgtacctg cttcagtatc gcgttccttc tcggcattaa
      361 ttcccggctt tattattctt tccgtgatgg ggattattgc ctgggcgttg aatacctggg
      421 gcaccaactt ccatcagatc attatggata ccatctcaac cccactggca tcgttgggta
      481 gcgtggtggg ctgggcctat gtgatctttg ttccactgct ctggttcttc ggtattcatg
      541 gcgcgctggc gctgaccgca ctggacaacg gcattatgac gccgtgggca ctggaaaata
      601 tcgcgaccta tcagcaatat ggttccgtcg aagcggcgct ggcagccggt aagaccttcc
      661 atatctgggc caagccgatg ctggactcct ttattttcct tgggggcagt ggtgcgactt
      721 taggcctgat cctggctatc tttatcgcct ctcgccgtgc tgattatcgt caggtggcaa
      781 aactggcgct gccgtccggc atcttccaga ttaacgaacc gattctgttt ggtctgccaa
      841 ttatcatgaa cccggtgatg tttatcccgt ttgtactggt acaaccgatt ctggcggcaa
      901 tcaccctcgc agcgtactac atgggcatta ttcctccggt gaccaatatt gcaccgtgga
      961 ccatgccaac cggtctggga gccttcttta acaccaacgg tagcgtcgcc gcattgctgg
     1021 tcgcactctt caaccttggc atcgcaacgt taatttatct gccctttgtt gtggtggcta
     1081 acaaagcaca aaatgcgatt gataaagaag agagcgaaga agatatcgct aacgccctga
     1141 aattttaatc gatgccggta cgggtaatcg tgccggtaag aaaagagagg aacgatgtat
     1201 gatggatctc gataacattc ccgatacgca aacggaagct gaagagctgg aagaagtggt
     1261 gatggggctg atcatcaact ccggacaagc gcgcagcctg gcgtatgcgg cactgaaaca
     1321 ggcgaagcag ggcgattttg ccgcagcaaa agccatgatg gatcagtcac ggatggcatt
     1381 gaatgaagcg catctggtac agacgaaact gattgaaggc gatgcgggcg aaggtaagat
     1441 gaaagtgagt ctggtgctgg tccacgctca ggatcattta atgacgtcca tgcttgcgcg
     1501 tgaactgatt actgaattaa ttgagcttca tgaaaaactg aaggcataag gagtcgatga
     1561 tgcagccagt gattaacgcg ccggaaattg ccactgcccg agaacagcag ttgtttaatg
     1621 gcaaaaactt ccatgtgttt atctataaca aaactgagag tatcagcgga ctgcatcagc
     1681 acgactatta tgaatttact ctggtattaa ccgggcgtta tttccaggag attaacggta
     1741 agcgcgtgtt actggaacgg ggcgattttg tttttattcc gttaggttcg caccatcaaa
     1801 gtttttatga gtttggtgcc acgcgcatat tgaacgttgg gatcagtaaa cgcttttttg
     1861 agcagcatta cctgccattg ttgccttatt gctttgtcgc ttcgcaggta taccggacca
     1921 ataacgcttt tctcacctat gtggaaacag tgatttcttc attgaatttc cgcgaaacag
     1981 ggctggaaga gtttgttgag atggttactt tttatgtcat taaccgttta cgtcattacc
     2041 gcgaagaaca ggtgattgat gatgtaccgc agtggctgaa aagtacggta gaaaagatgc
     2101 atgataaaga gcagtttagt gaatcggcgc tggagaatat ggtgacgttg tcagccaaat
     2161 cacaggaata tttgacgcga gcgactcaac gatattatgg caaaacgcca atgcagatta
     2221 ttaatgaaat ccgtattaat tttgccaaaa aacaactgga aatgaccaac tattcagtga
     2281 cggatattgc gtttgaggcc ggttatagta gcccgagttt gtttattaaa acgtttaaga
     2341 aattaacgtc ctttacgcct aagagctatc gtaagaaatt gactgaattt aatcagtaag
     2401 tcgttatacc tgacaattca catatctgtc ctgttgctgg attatttatg tccggggggg
     2461 cagatatgcc agatatcagt attctgtact gaagggagaa attatgagcc agaaattaaa
     2521 agtcgtcact attggtggcg ggagcagcta taccccggag ttactggaag gatttattaa
     2581 gcgttatcac gaattgccgg tcagcgaatt atggctggtg gatgtcgaag gtggtaaagc
     2641 gaaactggat attattttcg atctctgcca acggatgatt gataacgctg gcgtcccgat
     2701 gaagctttat aaaacgctgg atcgccgcga agcattgaaa gatgctgatt tcgttactac
     2761 ccaactgcgc gttggccaat taccggcgcg tgaactggat gaacgtattc cattaagtca
     2821 tggttatctt ggtcaggaaa ccaacggcgc gggcggtttg tttaaaggtc tgcgtaccat
     2881 tccggtgatt tttgacatcg taaaagatgt cgaagaactt tgtccgaatg catgggtgat
     2941 taacttcact aacccggcgg gaatggtcac tgaagccgtt tatcgtcata ccggatttaa
     3001 acgctttatc ggcgtttgta atattccgat cggcatgaag atgtttattc acgatgttct
     3061 gatgctgaaa gacagcgatg atttatctat cgatttgttc ggcctcaacc atatggtgtt
     3121 cattaaggat gtgctggtaa atggcaagtc gcgctttgcc gaattgcttg atggtgtggc
     3181 gtcagggcag ttaaaagcgt cctctgtaaa aaatattttc gatctgccat ttagtgaggg
     3241 cttaattcgt tcgttgaatc tgctgccatg ttcttatctg ctgtattact tcaagcagaa
     3301 agagatgctg gctattgaaa tgggcgaata ctacaaaggc ggcgcacgag cgcaggtagt
     3361 acagaaagtc gagaaacaac tttttgagct gtataaaaac ccggagttga aagttaagcc
     3421 gaaagaactg gaacagcgcg gtggggctta ttactctgat gcagcgtgcg aagtgatcaa
     3481 cgctatctac aacgacaagc aagcagaaca ttacgttaat atcccgcatc atgggcatat
     3541 tgataatatt ccggcagact gggcggtaga aatgacctgt aagctggggc gcgatggcgc
     3601 gacgccacat ccgcgcatta cgcatttcga tgataaagta atggggctga ttcacaccat
     3661 taaaggcttc gagattgctg ccagtaacgc cgcacttagc ggagaattta acgatgtgtt
     3721 actggcgcta aaccttagtc cgttggtgca ttccgatcgc gatgctgagc tgctggcacg
     3781 cgagatgatt ctggcgcacg agaaatggct gccaaacttt gccgactgca tcgcagagct
     3841 taaaaaagca cattaaccga ggctgattat ggaacgctta ctgattgtta atgccgatga
     3901 ttttggctta agcaaaggtc agaactacgg cattatcgag gcctgtcgca atgggattgt
     3961 cacgtcgacg acggcgctgg tgaatgggca ggctattgac catgcggtgc agttgagtcg
     4021 tgatgaaccg agtctggcca tagggatgca ctttgtcctt actatgggta agccactgac
     4081 agctatgccg gggttaaccc gcgatggtgt gctgggaaaa tggatctggc agttggcaga
     4141 agaagatgct ttaccgctgg aagaaattac tcaggagctt gccagtcagt atttgcgttt
     4201 cattgagcta tttggacgca aacctacgca tcttgatagc catcatcatg tgcatatgtt
     4261 cccgcagatt ttcccgattg ttgccaggtt tgcggcagag cagggcattg cgttgcgggc
     4321 ggatcgtcag atggcgtttg atttgccggt caacctgcgc actacccagg gattcagcag
     4381 tgcattctac ggtgaagaga tcagtgagtc gctgttcctg caagtgctgg atgacgcagg
     4441 ccatcggggt gatcgttcgc tggaggtgat gtgtcatccg gcgtttatcg ataatactat
     4501 ccgtcagagt gcttactgtt ttccacgctt aacggagctg gatgtgttga catcagcgtc
     4561 gttgaaaggt gctatcgctc agcgtggtta tcggttgggg agttatcggg atgtgtaagt
     4621 gaggatgttt gccagatgcg gcgtgaacct tgcatccggc gattaattcc tacaaaggcg
     4681 gcgtaactgc gccgcttgag a
//
LOCUS       DACAPB010000262         4564 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.266, whole genome shotgun sequence.
ACCESSION   DACAPB010000262 DACAPB010000000
VERSION     DACAPB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.266"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(91..330)
                     /locus_tag="IT904_004411"
     CDS             complement(91..330)
                     /locus_tag="IT904_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAP3194095.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRVGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(333..1052)
                     /gene="sanA"
                     /locus_tag="IT904_004412"
     CDS             complement(333..1052)
                     /gene="sanA"
                     /locus_tag="IT904_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAP3194096.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVAPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1202..2086)
                     /gene="cdd"
                     /locus_tag="IT904_004413"
     CDS             complement(1202..2086)
                     /gene="cdd"
                     /locus_tag="IT904_004413"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP3194097.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVASLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2216..2911)
                     /locus_tag="IT904_004414"
     CDS             complement(2216..2911)
                     /locus_tag="IT904_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAP3194098.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2908..3306)
                     /locus_tag="IT904_004415"
     CDS             complement(2908..3306)
                     /locus_tag="IT904_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP3194099.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3545..4495
                     /gene="dusC"
                     /locus_tag="IT904_004416"
     CDS             3545..4495
                     /gene="dusC"
                     /locus_tag="IT904_004416"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAP3194100.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         1230 a         1150 c         1164 g         1020 t
ORIGIN      
        1 tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa gtaataatta
       61 ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat aaacaatcat
      121 gccgatagat aaaattccga agccgatata tgccaccata taagcgaaaa agagtccggg
      181 attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt tatcaatcgc
      241 gcctacgcgc cagcctttcc atgcttcgcg gaaacaaaat atcggaataa ggaaaaaaac
      301 ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt ttgtaattca
      361 agtaactgtt cgggtgtgac ggcgggatac ccctgcgcat cttccggcac ctggtgcata
      421 gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat ataaaggtca
      481 gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat atctttcggt
      541 gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa taatgcccgc
      601 tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac tttgcgtgta
      661 cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc tgatggggcg
      721 acaccagcag cgattaaatc tttgcgcatg gtcatcggct cattataact ttgcaatgcg
      781 ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt aatcgctcct
      841 tgaatgcggt agcgataata ctgattaata acgccggtac gataatattt tgctgtgccg
      901 agcaccacgc cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat ataaggcgcg
      961 gttttccagc tcatccagcg atcgaggccg agcacagtca acagcagcaa gccgatcagg
     1021 actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgatctaa ggaaaatttc
     1081 catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt gccgggaatt
     1141 tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc cggcaccagg
     1201 cttaagcgag aagcacgcgg tcgatactgt gacagccgag agctttcagc gttgcggagg
     1261 tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg cgctggatat
     1321 ccgggtaatc ataacccttg agattcaaca gaattaacgc tccctgcaac ggtggcagag
     1381 tcgggttgaa tgcggcgttt tcagcgtagc tgccactgaa aatacgaccg tctttacatt
     1441 ccagcgcgac accgcttggc gacttactgt aaggcatgtg cgaacggttt gccgctgcaa
     1501 tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc tcgtccatta
     1561 gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag tcacgcagcg
     1621 cgtgtgcctc gcggcccggc agatgaatac gcagatccag accgctgttc agttcattca
     1681 taaactgacg gcagtgacca caaggcgtgt agttaacggt gatggctgca agcgcttttt
     1741 caccactcaa ccaggcgtgg ctgatcgcgc tttgttcggc atgaacggtt tgctgcattg
     1801 tcgcaccaat aaattccata ttggcaccaa aataccaggt tccgctcaca ccgcgcgcaa
     1861 ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgccgcc agcggaagta
     1921 gtgcgaatgc cagcgcgtct tcgtccagcc ccgttgcgct cttcagcgat gcgacttgct
     1981 ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt gcagattgca
     2041 agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg ttgcctcata
     2101 ttcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga tctgaatctc
     2161 attaattatg caacacgtga aacttacatc aaaaatgcgc gacgtatcgc aaattttagc
     2221 ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta attatcccgc
     2281 ataacaccag cgccagcgaa ctaaatgcac cttcctgata atccagctcg gcgcagcgcg
     2341 ccgtaccgag ggcgtgcgag gctgtcccca ttgccagacc gcgcgcagct ttggtacgaa
     2401 tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg acgaaaatca
     2461 cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc attgcaatag
     2521 gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg cccatcaaca
     2581 atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag atggtgatga
     2641 tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag gccagcgcga
     2701 caaccgccgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtag ctgtcgtaag
     2761 agatgccggt cagcattaaa aaagggatga tcaccaccat tgctaccagc aacgggttaa
     2821 gcaagggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagaca atcaatgtca
     2881 gtggtaatga ccaccagata ttcgccatca ttcttctgat cctttctgac ctacgacttt
     2941 acgttcaccg tgcaccagtt gcgaactcca gctcaccacc agaaaaacca ccagcgtact
     3001 gacagcacag gaaaccacta ccgggccaaa ctgtgcgcgg agcaaatcaa aatattgcat
     3061 gacgcctacg ccaatcggca caaacaatag cgccatatag cgaatcagta cgtagcaccc
     3121 cggattgacc cattttgccg gaagaatttg caaggccagc aggacaaaca ggatcagcat
     3181 cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa tgcctgcata
     3241 caggcaggca taaatcagga cgaaagcgcg taaatattgc cagataatgt tcagtgtctt
     3301 actcataatc aacaccccag atgagatgga ttcatcatac aattaattcg taaaacgtgc
     3361 taccgatcac attaagaaat cagcgcttat tttttcgctc cccggtatta cgcaatgagc
     3421 agcgaactca ggcatacatc aagcatagag agggattgca gtttgcagca atccctcttt
     3481 gcagaccttt aatccgctga ccataggcgc taacatagcg cctcattttt ttgcgggtga
     3541 tgatatgcgt gtgttactgg caccgatgga gggagtgctt gactctctgg tgcgtgaatt
     3601 gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg tggtggatca
     3661 actgctgccg gtaaaagtct ttcatcgcat ttgccctgag ctacaaaacg ccagccggac
     3721 accatctggt acgctggtgc gcgtgcagtt gttaggtcag ttcccacaat ggctggcaga
     3781 gaacgccgcc cgtgcggtgg agttaggttc ctggggcgtg gatctcaatt gcggctgccc
     3841 gtcgaaaacg gttaacggta gcggcggcgg cgcgacgtta ctcaaagatc ctgaactcat
     3901 ctaccagggt gcaaaagcga tgcgtgaagc tgtaccagcg catttgcccg tcagcgtaaa
     3961 agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca
     4021 ggctggcgct acggagctgg tggtgcatgg gcggacaaaa gagcagggtt accgcgcgga
     4081 gcatattgac tggcaggcga ttggcgagat tcgccagcgg ctgaatattc cggtgattgc
     4141 caacggtgaa atctgggact ggcagagcgc gcaacaatgc atggcgatca gcggctgcga
     4201 cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg tggtaaaata
     4261 taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat atacccgtct
     4321 ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg attaaacagt ggttgagtta
     4381 tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg tgttgaataa
     4441 ttcccctgat attgcaaggg ctattcaggc aattgatatc gataagctgc gataatagtg
     4501 gaatcgcagt ttatcagaga caatgaatcg cttattcagc ttcatcatct tgttgcgctc
     4561 cttt
//
LOCUS       DACAPB010000263         4561 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.267, whole genome shotgun sequence.
ACCESSION   DACAPB010000263 DACAPB010000000
VERSION     DACAPB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.267"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            239..571
                     /gene="qac"
                     /locus_tag="IT904_004417"
     CDS             239..571
                     /gene="qac"
                     /locus_tag="IT904_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /protein_id="HAP3194101.1"
                     /translation="MKNWLFLAIAIFGEVVATSALKSSHGFTKLVPSVVVVAGYGLAF
                     YFLSLALKSIPVGIAYAVWAGLGIVLVAAIAWIFHGQKLDLWAFVGMGLIVSGVAVLN
                     LLSKVSAH"
     gene            complement(836..1471)
                     /locus_tag="IT904_004418"
                     /pseudo
     CDS             complement(836..1471)
                     /locus_tag="IT904_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1473..1748
                     /locus_tag="IT904_004419"
     CDS             1473..1748
                     /locus_tag="IT904_004419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194102.1"
                     /translation="MNIGLFGFTNKHYYLATKTQEKYTKPFGSDGSLSPLDYSVGIGT
                     GLELGHFQFMFGYDYPLSNSSKDPDLSQVRQHNFRASIAYSFRKLKK"
     gene            complement(1751..2542)
                     /gene="sul3"
                     /locus_tag="IT904_004420"
     CDS             complement(1751..2542)
                     /gene="sul3"
                     /locus_tag="IT904_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul3"
                     /protein_id="HAP3194103.1"
                     /translation="MSKIFGIVNITTDSFSDGGLYLDTDKAIEHALHLVEDGADVIDL
                     GAASSNPDTTEVGVVEEIKRLKPVIKALKEKGISISVDTFKPEVQSFCIEQKVDFIND
                     IQGFPYPEIYSGLAKSDCKLVLMHSVQRIGAATKVETNPEEVFTSMMEFFKERIAALV
                     EAGVKRERIILDPGMGFFLGSNPETSILVLKRFPEIQEAFNLQVMIAVSRKSFLGKIT
                     GTDVKSRLAPTLAAEMYAYKKGADYLRTHDVKSLSDALKISKALG"
     gene            complement(3011..3256)
                     /locus_tag="IT904_004421"
     CDS             complement(3011..3256)
                     /locus_tag="IT904_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAP3194104.1"
                     /translation="MKIEIMEYNPDWTKNFEEEKIKLLHFFGSHAVAIEHIGSTAIPN
                     QRAKPVIDIFIGVSPFAELPFISAFLMQRSITTLRQI"
     gene            complement(3294..4157)
                     /locus_tag="IT904_004422"
     CDS             complement(3294..4157)
                     /locus_tag="IT904_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3194105.1"
                     /translation="MIPNSENKRVWFITGASKGLGYAFTCAALKAGDKVVAVARTIDN
                     LAKLEETYQESLLPLNLDVTDREAVFSTVETAVKHFGRLDIVVNNAGIMTMGMIEELN
                     ESDARKLMDTNFFGALWVCQAVMPYLRSQRSGHIIQITSIGAIISGPMSGIYSASKFA
                     LEGMSEALAKEAEHFGVKLTMVEPGGYWTDLYTSMSYSNPLDSYGTLRDELAKQYSED
                     SVDSDPSLAAEALMKLVASNNPPLRLILGSMVYDLAMDTLKARMATWEEWEAVSRASE
                     KAIPAPERYGV"
     gene            complement(4303..4542)
                     /locus_tag="IT904_004423"
     CDS             complement(4303..4542)
                     /locus_tag="IT904_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3194106.1"
                     /translation="MAYVQESIAPEMMGKVFSLLMTAMTLSMPIGLLVAGPVVEVIGV
                     NTWFFWSGVALIVNAVLCRILTRRYDKVTMKPQVD"
BASE COUNT         1263 a         1101 c          891 g         1306 t
ORIGIN      
        1 cgcgcagatc agttggaaga atttgttcac tacgtgaaag gcgagatcac caaggtagtc
       61 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
      121 agcgttagat gccagatttg gcgttgcgta tgctcacaga aaatcgacag ccgcaagact
      181 gtttttggca actcatagcc accactattt atccttcata ccgtagagga gattgcacgt
      241 gaagaactgg ctctttctgg ctattgcaat atttggtgag gtcgtcgcaa cttccgcact
      301 gaagtccagc catggattca ccaagttagt tccttctgtt gtagttgtgg ctggctacgg
      361 gcttgcgttc tatttcctct ctctcgcact caagtccatc ccggtcggca ttgcttatgc
      421 tgtttgggct ggcctcggca tcgtacttgt ggcagctatc gcttggatct tccatggcca
      481 gaaactagac ttgtgggcgt tcgttggcat gggacttatc gttagtggcg tcgccgttct
      541 aaatctgcta tccaaggtca gcgcacattg atcgggctgg catctaacag ttcattcaag
      601 ccgacgccgc ttcgcggcgc ggcttaattc aggtgttagt cggacgccta cggcgccgag
      661 gaaaaaagaa accaaaccat tcttgctgcc aaagagaaaa accccttacg gtggcgtact
      721 gggaaagtgc gtatctacga cgccttgccg gtctcaatgt catgccgtcc tctttcgaag
      781 gctgcttgat gcaaacctct caaaaatctg gcataaacta tcttgacata ttccgattga
      841 acaccttccg aatatcagcg gctatcagcg ggatctcatc ttttttcgtg acgtaggagt
      901 gggcattctg ctgcagatga aactggcagc gttgccacag aattccaggg aagacagcat
      961 cgattgccgc gcgcagtcct gagtggtcat cactggtgat cattcgcagg ccgtgcatcc
     1021 ctcgtcgtac gagaccctcg agaaaggaac gccagtttac ctctgcctca ctgttcgcga
     1081 cttcggcgtc aagaatatgc cgattcccgc tgtaatcaac gccaatagcc actagaagcg
     1141 cctgcttgac cacatgggaa ccgacgcgaa ccgattcata ggtcgcatca agtaccaagt
     1201 attgaatctg tccgacaggc tgcgccttcc aagacgtaat ctcttcatcg agctcctttg
     1261 ccagcctgct gacctgggac gagctgactt cggtgccaca aagaatttcg acgatatccg
     1321 agaccctgcg ggtacttact cctttgacat acatttcggc gatggcgagc ttgagagcac
     1381 gctcactgcg catgcctttc tcgatgcagc tgggataaaa ctccatcccg cgaacttgtg
     1441 gtactttcaa gaggactttc ccggtcgcaa gattgaatat tggattattt ggatttacca
     1501 acaaacacta ctatttagct acgaaaactc aagagaagta cacaaaacct tttggaagtg
     1561 atggaagttt aagtcctctt gattatagtg taggtattgg aacaggttta gaattaggac
     1621 atttccagtt tatgtttggc tatgattacc ctcttagtaa cagttcgaaa gatccagacc
     1681 taagtcaagt acgccaacac aacttcagag caagtatagc atattcattt agaaagctaa
     1741 aaaaataaaa ctaacctagg gctttggata ttttcaaggc atctgataaa gacttaacat
     1801 catgggtgcg gagataatct gcaccttttt tgtatgcata catttctgct gcaagagttg
     1861 gtgctaaacg agatttcaca tcggttccag ttattttacc taagaatgat ttccgtgaca
     1921 ctgcaatcat tacttgcaaa ttaaaagctt cttgaatttc agggaaacgc ttcaaaacaa
     1981 gaatagatgt ttctggatta gagcctaaaa agaagcccat acccggatca agaataattc
     2041 gttcacgctt tacaccagcc tcaactaaag cagcaattct ttctttaaaa aattccatca
     2101 tggaagtaaa aacctcttcc ggattcgttt caactttagt agctgcacca attcgctgaa
     2161 cggagtgcat caacacaagt ttgcaatctg actttgccaa gcctgaataa atctcaggat
     2221 aaggaaaacc ttgaatatca ttaataaaat caaccttttg ttctatgcaa aaactctgaa
     2281 cctcaggttt aaatgtatca acagaaatag aaatgccttt ttcttttaaa gccttaatga
     2341 caggtttgag tcttttgatt tcttccacaa cgcccacttc agttgtatca ggattactgg
     2401 aagcggctcc caaatcaatc acatctgctc catcttcaac caaatgcaga gcatgctcaa
     2461 ttgccttatc tgtatctaaa taaagtcctc catcggaaaa actatcggtg gttatattta
     2521 cgattccaaa aatcttgctc atatcatttt gctttgatta caaaagtagc atttatctta
     2581 attgactaat tatcacttac gcaaattgac acattttccc tatctttgca gcctaatttt
     2641 tttgttttaa ttcattatga aaactgatag gaactgtcat ttcgagtttt tcgcggtttt
     2701 gggtcaaaat tcggtttttt ccccgaacta cgactgtttt cgcaccccga acaactaatc
     2761 caatcagaat tttccggcaa cgccgactcc aagaagtgta attccagtct atttctgccg
     2821 tcaacccctg attcgcgtta atgcgacaaa gctcaaacac ataactcccc cacgaagcca
     2881 tataaactcc gatttcgagg tgttatcctg ttccgcataa acttcgtttt tgggtattat
     2941 acctacacca aagcaagaag attatgggtc caaacttcat ttgtatattt tgcaaacaaa
     3001 tatctgcctg tcatatctgt cggagtgtgg tgatactcct ttgcattaaa aatgcgctga
     3061 taaaaggtag ttcagcaaaa ggcgaaacgc caataaatat atctataacc ggtttcgccc
     3121 gttgattggg tatcgccgtg cttccgatat gttcaatggc tacggcatga gaaccgaaaa
     3181 aatggagcaa ttttattttc tcttcttcaa aattctttgt ccaatccggg ttatattcca
     3241 taatttcaat tttcatgata gtttcgtcct tgcctcaaaa accatgtttg gtattatact
     3301 ccatatctct ccggtgcagg aatagccttt tctgatgcac ggctaacagc ttcccattct
     3361 tcccaggtag ccatccgtgc ttttaaggta tccatcgcca aatcgtatac catgctgcca
     3421 aggataagcc tcaaaggcgg attattgctg gctactaatt tcataagggc ttccgctgcc
     3481 aaggaaggat cactatcgac cgaatcttcg gaatactgtt tcgccagctc atcgcgcagt
     3541 gtaccgtatg aatctaatgg attgctgtaa ctcatcgaag tgtataaatc tgtccaatat
     3601 cctcctggtt ccaccatagt gagtttcact ccgaaatgtt ccgcttcttt tgctaaggct
     3661 tcactcattc cttccagcgc aaatttactt gcactgtaaa taccagacat cggacccgag
     3721 ataatagcgc caatgcttgt aatctgtatg atatgtccag aacgctgcga cctcaaatag
     3781 ggcatcactg cctgacaaac ccaaagagct ccaaaaaagt ttgtgtccat tagtttccga
     3841 gcatcggatt cgtttagttc ttcaatcata cccatagtca tgatacccgc attattaaca
     3901 acaatatcaa gcctaccgaa atgtttaact gctgtttcaa ccgtagaaaa aacagcctcc
     3961 ctatctgtaa catcgaggtt taatggcagt aagctctctt gatatgtttc ttctagcttc
     4021 gccaaattat cgatagtcct tgcaactgca acaactttat ccccggcttt caaggcggcg
     4081 catgtaaaag catagccaag ccccttgctt gctccggtaa taaaccatac tcttttattt
     4141 tcgctattag gtatcataga tattctcctt taggctatta tagaaagatt attataagcg
     4201 aaattaatac cgttaaacgc catgagcccg acaattttga gcactttgtt ttgtactccc
     4261 agaagctcac cactacaaat tatcatcaac ccggtccttt tttcagtcca cttgcggttt
     4321 cattgttact ttgtcatagc gtcgtgtcag aatgcggcag agaacagcgt ttactatcaa
     4381 cgcaacacca gaccagaaaa accatgtatt aacacctata acctcaacaa ccggacctgc
     4441 aacaagtaag cctatcggca tagaaagagt catggcggtc atcaaaaggg aaaacacctt
     4501 gcccatcatt tcaggggcaa tgctttcttg aacataagcc ataacaggaa cattcataaa
     4561 t
//
LOCUS       DACAPB010000264         4551 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.268, whole genome shotgun sequence.
ACCESSION   DACAPB010000264 DACAPB010000000
VERSION     DACAPB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.268"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            272..322
                     /gene="hisL"
                     /locus_tag="IT904_004424"
     CDS             272..322
                     /gene="hisL"
                     /locus_tag="IT904_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAP3194107.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            468..1367
                     /gene="hisG"
                     /locus_tag="IT904_004425"
     CDS             468..1367
                     /gene="hisG"
                     /locus_tag="IT904_004425"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAP3194108.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1373..2677
                     /gene="hisD"
                     /locus_tag="IT904_004426"
     CDS             1373..2677
                     /gene="hisD"
                     /locus_tag="IT904_004426"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAP3194109.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVDSVGLYIPGGSAPLFSTVLMLATPARIANCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERLLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2674..3744
                     /gene="hisC"
                     /locus_tag="IT904_004427"
     CDS             2674..3744
                     /gene="hisC"
                     /locus_tag="IT904_004427"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAP3194110.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGIKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKKISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3744..>4551
                     /gene="hisB"
                     /locus_tag="IT904_004428"
     CDS             3744..>4551
                     /gene="hisB"
                     /locus_tag="IT904_004428"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAP3194111.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTALALGEALKIALGDKRGICRFGFVLPMDECLARC"
BASE COUNT         1093 a         1225 c         1223 g         1010 t
ORIGIN      
        1 ttttgtacag ttcattgtac aatgatagac gttaattaac tatttattaa ttagtttgta
       61 gatcaagaca ttgtcagtga gacgaaaaat ccaggcttcg ctatttttgg taccgtcagc
      121 ttagaggtca gtcctcttag ccccctcctt tccccgctca ttcattaaac aaatccatcg
      181 ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag gttaaaagac
      241 atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta aacaccacca
      301 tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca ttcagatctt
      361 ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta
      421 ttgcgcggtt gataacggtt cagacagatt taaagaggaa taacaaaatg acagacaaca
      481 ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca cgtgaattgc
      541 tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg atggcagaaa
      601 acatgccgat tgatattctg cgcgtgcgtg acgacgacat tcccggtctg gtgatggacg
      661 gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagaactg cttaaccgcc
      721 gcgcccaggg tgaggatcca cgctacttta ccctgcgtcg tctggatttc ggcggctgtc
      781 gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc ttaaacggta
      841 aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag aaaggtatct
      901 cttttaaatc ctgcttactg aacggttctg ttgaagttgc gccgcgtgcc ggactggcgg
      961 atgcgatttg cgatttggtt tccaccggtg ccacgctgga agctaacggc ctgcgcgaag
     1021 tcgaagttat ctaccgctcg aaagcctgcc ttatccagcg cgatggtgaa atggaagaat
     1081 ccaaacagca gctgatcgac aaactgctta cccgtattca gggtgtgatc caggcgcgcg
     1141 aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc atcgccctgc
     1201 tgccaggtgc cgaacgccca actattctac cgctggcggg tgatcaacaa cgcgtagcga
     1261 tgcacatggt cagtagcgaa accctgttct gggaaacgat ggaaaaactg aaagcgctgg
     1321 gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg ccatgagctt
     1381 taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc tgttaatgcg
     1441 cccggcgatc tccgcttctg aaagcattac ccgcactgtt aacgatattc tcgataacgt
     1501 gaaagcacgc ggcgatgagg ccctgcggga atacagcgcg aagtttgata aaaccacggt
     1561 taccgcgctg aaggtgtctg ctgaggaaat tgccgccgcc agcgaacgcc tgagcgacga
     1621 gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa accttccaca ctgcgcaaaa
     1681 actgccgccg gtagatgtag aaacgcagcc aggcgtacgt tgccagcaag tcacgcgccc
     1741 ggtagattca gtgggtttgt atattcctgg cggctccgcc ccgctcttct caacggtatt
     1801 aatgctggca actccggcgc gtattgcgaa ctgtaaaaaa gtggtgctgt gctcaccgcc
     1861 gccgattgcc gatgagatcc tttacgcggc gcagctgtgc ggtgtacagg acgtgtttaa
     1921 cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt
     1981 ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgag gcaaaacgtc aggtgagcca
     2041 gcgtctggac ggtgcggcga tcgatatgcc cgcaggccct tcggaagtgc tggtgattgc
     2101 tgacagcggc gcaacgccgg atttcgtggc ttctgatttg ctttcccagg ctgaacacgg
     2161 cccggactca caggtgattt tactgacgcc cgctgctgat atggcgcgtc gcgtagccga
     2221 agctgtcgaa cgcctgctgg cagaactgcc gcgagctgaa accgcccgcc aggcactgaa
     2281 cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtagagatct ccaaccagta
     2341 cggcccggag cacctgatca ttcagacccg caacgcccgc gatctggtcg atggcatcac
     2401 cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tccgcaggtg attacgcctc
     2461 cggcaccaac cacgttctgc cgacttacgg ttacaccgcc acctgttcca gcctcgggct
     2521 ggcagatttc cagaagcgta tgaccgtgca ggaactgtcg aaagaaggct tctccgcgct
     2581 ggcttcaacc attgaaacac tggccgccgc cgagcgcctg accgcccata aaaatgccgt
     2641 tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat caccgattta
     2701 gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaac
     2761 ggcgacgtct ggctgaacgc caacgaatac cccacagccg tggagtttca gcttactcag
     2821 caaacgctca accgctaccc ggaatgtcag ccgaaagcgg tgattgaaaa ttacgcgcag
     2881 tatgcaggcg taaaaccgga gcaggtgctg gtcagccgtg gcgcggacga aggtattgaa
     2941 ctactgattc gcgctttttg cgaaccaggt aaagacgcca tcctctactg cccgccaacg
     3001 tacggcatgt acagcgtcag cgctgaaacc attggcgtcg agtgccgcac agtgccgacg
     3061 ctggacaact ggcaactgga cttacagggc atttccgaca agctggacgg cataaaagtg
     3121 gtctatgttt gcagccccaa caacccgacc gggcaactga tcaacccgca ggattttcgc
     3181 actctgctgg agttaacccg cggtaaagcg attgtggttg ccgatgaagc ctatatcgag
     3241 ttttgcccgc aggcatcgct ggctggctgg ctggcggaat atccgcacct ggctattttg
     3301 cgcacactgt cgaaagcttt tgctctggcg ggccttcgtt gcggatttac gctggcaaac
     3361 gaagaagtca tcaacctgct gatgaaagtg atcgccccct acccgctctc gacgccggtt
     3421 gccgacattg cggcccaggc gttaagcccg caggggatcg tcgccatgcg cgaacgggtg
     3481 gcgcaaatta ttgcagagcg cgaatacctg attgccgcac tgaaaaaaat ctcctgcgtg
     3541 gagcaggttt ttgactctga aaccaactac attctggcgc gctttaaagc ctccagcgca
     3601 gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa acaaccctct
     3661 ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg cgtcattgac
     3721 gccttacgtg cggagcaagt ttaatgagtc agaagtatct ttttatcgat cgcgatggaa
     3781 ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa ctcgcctttg
     3841 aaccgggcgt gatcccagag ctgctgaagc tgcaaaaagc gggctacaag ctggtgatga
     3901 tcactaatca ggatggtctg ggaacacaaa gtttcccgca ggcggatttc gatggcccgc
     3961 acaacctgat gatgcagatc ttcacctcgc aaggcgtaca gtttgatgaa gtgctgattt
     4021 gcccgcacct gcccgcagat gagtgcgact gccgtaaacc gaaagtaaaa ctggtggagc
     4081 gttatctggc tgagcaagcg atggatcgcg ctaacagtta tgtgattggc gatcgcgcga
     4141 ccgacattca actggcggaa aacatgggta ttaatggttt acgctacgac cgcgaaaccc
     4201 tgaactggcc gatgattggc gagcaactca ctaaacgaga ccgttacgcc catgtagtgc
     4261 gcaacaccaa agagacgcag attgacgttc aggtatggct ggatcgcgaa ggtggcagca
     4321 agattaacac cggcgttggc ttctttgatc atatgctgga tcagatcgcc actcacggcg
     4381 gtttccgcat ggaaatcaac gtcaaaggcg acctctatat cgacgatcac cacaccgtcg
     4441 aagataccgc tctggcgctg ggcgaagcgc taaaaattgc cctcggagac aaacgcggta
     4501 tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc g
//
LOCUS       DACAPB010000265         4475 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.269, whole genome shotgun sequence.
ACCESSION   DACAPB010000265 DACAPB010000000
VERSION     DACAPB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.269"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(787..2235)
                     /gene="cusS"
                     /locus_tag="IT904_004429"
     CDS             complement(787..2235)
                     /gene="cusS"
                     /locus_tag="IT904_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAP3194112.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(2225..2908)
                     /gene="cusR"
                     /locus_tag="IT904_004430"
     CDS             complement(2225..2908)
                     /gene="cusR"
                     /locus_tag="IT904_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAP3194113.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3065..4438
                     /gene="cusC"
                     /locus_tag="IT904_004431"
     CDS             3065..4438
                     /gene="cusC"
                     /locus_tag="IT904_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAP3194114.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIDIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
BASE COUNT         1201 a         1091 c         1075 g         1108 t
ORIGIN      
        1 tccggcggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc
       61 agtaaaatga ataatgatcc gtaccgtaca actgcggata atgtgataag cgggacgact
      121 tcagacgtta ttagggaagg aacagaaaat gctatggtta tgtgagaatt ttgtttaaat
      181 attactttac agaaacgggt gtctgttcag tcaaaaaaat aatcacaagc tcaacctctt
      241 gtttcttggc gttattcagg acaggtacgc atatcacaac gagtaacaaa aacgtaaagt
      301 taaaccaata ttcataatat atttttattc acagcaataa ataatccaat taacattgtt
      361 aaaatgaatg attgcataag aaatatatgc taccgctcca ccctgataac atgatctgca
      421 cattgcacat cttaacgttg ttaaatactg gctcaatagt ggatatttta aattaggaat
      481 ttatcaaatt cacttgataa atagattgag tgaataatat aacaattaat gatgattaat
      541 cgcgattaaa ttattcattg ctttatatga tagatggctt gtttataacg atgaaaattt
      601 agaaagggca ttgtgatcta caattggaag aaacacagat ttaactaagt ttcatataca
      661 aacatctcgt gaaactttga aaaataaaag ccttcgatat ttcatatgtt taataagttt
      721 ttctctaatt tgttgatcaa caataccagg aggtgatgca ccacgccctg aatttgaact
      781 taataatcac tctctctccg gcagcataat cacaaacctt gtcccccgcg catctgacgt
      841 taccgcaacc gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc
      901 gctaccttca ccttttcgct ggcgggaagg gtcaacgcga tagaaacggt caaacaatcg
      961 cggtaagtgc tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctgatg
     1021 attgaccgtc tggcagcgca ctacaatagc ctcactgggt ggcgtataac gcagggcatt
     1081 agaaagcagg ttgcttaacg cccgacgcag catcagcgga tcgcccgcaa cctgacattt
     1141 gtcgccaaca aatcgcaact ccacgccgcg atcttccgct aacgcctcga aaaaatcgaa
     1201 cactttgccg acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt
     1261 atcggcctga gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc
     1321 gagattagag tagagcacat cttccagctc cttctggctg cgagactggc tgagggcgat
     1381 ttccgtttgc gtgatgagat tggtaatcgg cgtgcgaatt tcgtgagcga tatccgctga
     1441 gaaattggac tggcgggtaa agacatcctc aatacgctcg atcatatggt tgaacgacag
     1501 taccagctgt tccagctcaa tcggcacggt ctgcgggtcg aggcgaacgt cgagatcttt
     1561 cgaggtaata ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac
     1621 cgccaacagt acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt
     1681 attcatcaaa tcatttatgt aatgaagatg aaaatcgatc gaaagcgcga tgtagagcgt
     1741 ataaatcggt ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct
     1801 gtgttccata tgcccgtgac cgtggcctgg catcatcatc gtcgggccgg aaaggagata
     1861 cacctcgcca ccctgagcgt ctttatcggg tatggcgtca cgcgtaaact cgcggatatc
     1921 cggcgcaccg ggggagtgat acaccgtttt accctgacta tctgccaggg aaatcaacac
     1981 attggaataa ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc
     2041 agggtgattt agtacccgtt caagggtggc gctaatctct tttaaatcat taatatcctg
     2101 ctcggcaaaa tgaactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc
     2161 gatggtggcc aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa
     2221 tggcttactg accatccggc acctcaagca tgtaacccac gccgcgcacg gtctgaatta
     2281 gcttcggctc aaagtcgttg tcgattttgc cgcgcagccg cttcaccgcc acatcaatag
     2341 cattggtatc gctgtcaaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca
     2401 gcacttcgcc ctgatggcga aggaagaact ccagcagagt aaactcttta ctggtcaaag
     2461 tgatgcgcgt gccgctgcgg gtgactttgc ggctgacgag atcgaccatc aaatcggcaa
     2521 cctgaaactg actttcgata atcaccgccg ccccgcgccg cagtaatgtg cgcacccgcg
     2581 ccagcagttc agcaaaagcg aatggcttca ccaggtagtc atctgccccc aactccagcc
     2641 ccttgacgcg atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat
     2701 tggcggagcg taacatgcgc acgatatccc agccgttcac gtccggcagc ataatatcga
     2761 ggattatcag atcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg
     2821 ccaaatcgac cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct
     2881 tttcatcttc gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag
     2941 agtagcggga tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt
     3001 caccggaaaa tcagagcctg gcgagtaaag ttggcggtat aaaatcacca gaaattatga
     3061 gcctatgtct ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ccggttgttc
     3121 actggcaccg gattatcagc gtccggcaat gcccgtgccg cagcagttct cactcagcca
     3181 gaacggcctg gttaacgcag cagataacta tcagaacgcg ggctggcgca ccttttttgt
     3241 tgataatcag gtgaagacgc tgattagcga ggcgctggtg aataatcggg atttgcgcat
     3301 ggcgacgctg aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta
     3361 cccacagctc aatggcgagg gcagcggcag ctggagcggt aatcttaaag ggaataccgc
     3421 cacgacacgg gagttttcga ccggccttaa cgccagcttt gatctcgatt ttttcggtcg
     3481 cttaaagaac atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg
     3541 cgcggtgcat attttgctgg tttctaatgt cgcgcaaagt tatttcaatc agcagctagc
     3601 gtatgcgcaa ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt
     3661 tgtcgaaaaa caactgttga caggtagcag taatgttctg gcgctggaac aggctcgcgg
     3721 ggtgattgaa agtacccgca gcgacatcgc taaacgtcag ggggaactgg cgcaggcgaa
     3781 taatgcattg caactgttat tgggaagcta cggcaagctg ccgcaagcgc agacagtaaa
     3841 cagcgacagc ctgcaaagcg ttaaattacc ggcgggattg tcgtcgcaaa tcttattgca
     3901 gcgcccggat attatggaag ctgaacacgc gttaatggcg gctaatgcca atattggcgc
     3961 tgcacgcgcg gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag
     4021 cgatctgtcg tcattattta acgccagcag cgggatgtgg aattttattc ctaaaattga
     4081 tatccccatt tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca
     4141 gcagcagtcg gtggtgaatt atgaacagaa aatccagaac gcctttaaag aagtggcaga
     4201 tgcgctggca ttacgtcaaa gcctgaacga tcaaattagc gcccagcagc gttatctggc
     4261 atcgctgcaa attactttgc aacgggcgcg ggcattatat cagcacggcg cagtaagtta
     4321 tctggaagtg ctggatgccg agcgttcttt atttgcaacc cgacaaactt tactcgacct
     4381 gaattatgcc cgtcaggtta acgaaatttc tttgtatacc gcacttggtg gcggttgaca
     4441 gcaatatact cgtcatactt caagttgcat gtgct
//
LOCUS       DACAPB010000266         4453 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.270, whole genome shotgun sequence.
ACCESSION   DACAPB010000266 DACAPB010000000
VERSION     DACAPB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.270"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..120
                     /locus_tag="IT904_004432"
     CDS             <1..120
                     /locus_tag="IT904_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP3194115.1"
                     /translation="KLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(92..535)
                     /locus_tag="IT904_004433"
     CDS             complement(92..535)
                     /locus_tag="IT904_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP3194116.1"
                     /translation="MMKYLYVNNLAYPYELKSLYVEKGEWPEEKGVDIDEVIFREYFY
                     DTPPEGKYRCVGEDGLPAWVDIPPPTREEQIASAETKKQQLINQANEYINSKQWPGKA
                     SIGRLKGEELVQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(535..909)
                     /locus_tag="IT904_004434"
     CDS             complement(535..909)
                     /locus_tag="IT904_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3194117.1"
                     /translation="MMPFARYFCIFINVGLGEAAKRNVGTGENQIPDMSSYASGSGWR
                     KMPDGSIEQWGRISFPSEHGPVSANVSFPIPFTQTPGIVIVCDGGFGGGNMWGATNWS
                     TTGFIAHCNYGLEGGAFFAKGW"
     gene            936..1058
                     /locus_tag="IT904_004435"
                     /pseudo
     CDS             936..1058
                     /locus_tag="IT904_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1160..3049)
                     /locus_tag="IT904_004436"
     CDS             complement(1160..3049)
                     /locus_tag="IT904_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP3194118.1"
                     /translation="MKSETLTVQQLFQDRRQYCVPFYQRAYVWTQRNQWSALLEDILE
                     KAQSRLSGTKPTPHFLGAVVLEPQPKKGLLGVDSIHIIDGQQRLTTLQYVLASIRLAL
                     RATDLSNLGPLISSCLKNSNEDTMRNKEVERFKLWPTFRDQTHFIQSFNVENIDDIRN
                     VFSDSFTQHGTLRKHFNHPPSLEALCFFTEAFIKWIKIENHSPRENAVALIEAVLTDL
                     KLVSIFLEAEDDAQIIFETLNGRGAELHATDLIRNYIFMCAEHENINAVELYENEWKI
                     FEDTYWSERQRRGRINKPRMEWLVHATLQSERQREIDLSRLYNEYRDYVSKDLPSQRA
                     DLQVKRLKQYASQYKELVGGFGTTPISHFGHRIAAYDVTTLYPLALFISIANIADDEK
                     AAMYNDLVSYVVRRAVCGLTPKNYNNVFMNVLRHLSKTEISSVELRNILNSLNGEASR
                     WPGDSEFLNACINAPLYPGRLDAPKMRSMLTELERELCRQVKTEKPDVPNLSNLDIDH
                     LMPQSWYSCWPLENGHMVTNSDATVLNQIVLSGTDLTPEQLLVRKRQQAIATLGNLTL
                     LNLSVNRSVQNAVFLKKRDALIVHTNLRLNIPLIVKDKWDEDEILERGKKLGEIALKV
                     WPKHD"
     gene            3487..3702
                     /locus_tag="IT904_004437"
                     /pseudo
     CDS             3487..3702
                     /locus_tag="IT904_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3905..4237
                     /locus_tag="IT904_004438"
     CDS             3905..4237
                     /locus_tag="IT904_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein flxA"
                     /protein_id="HAP3194119.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNEDATVHPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            4320..>4453
                     /locus_tag="IT904_004439"
     CDS             4320..>4453
                     /locus_tag="IT904_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP3194120.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     "
BASE COUNT         1246 a          972 c          953 g         1282 t
ORIGIN      
        1 aagctgacgc aggcagaaac cacccgactt aacgccgtgc tggattacat tgacgcggtg
       61 acggcaacag ataccagcac tgcgccggat gtcatctggc ctgaactgcc ggaggcgtag
      121 gccattcaat atctggagca ctggaggtat caaccagttc cagtgcgtcc agataatcca
      181 gccacaaatt atattgcacc agttcctcac ctttcagacg accaattgat gctttacctg
      241 gccattgttt actgttgatg tattcgttgg cctgattaat caattgctgc tttttagttt
      301 cggctgatgc aatttgttct tcacgtgttg gtggaggaat atctacccat gcaggcagtc
      361 catcttctcc gacacatctg tattttcctt ctggtggtgt atcatagaaa tattccctga
      421 aaattacttc gtctatatca acaccttttt cttcaggcca ctcacctttt tcaacataaa
      481 gagacttaag ttcgtaagga tatgccaggt tgtttacgta cagatatttc atcattacca
      541 gcccttagcg aaaaacgcac caccttcaag accataattg cagtgagcta tgaagccggt
      601 agtgctccag ttggtcgccc cccacatatt cccacccccg aaaccaccat cgcacacaat
      661 tacaatgccc ggtgtctgtg taaatggaat cgggaatgaa acattggcgg atacaggccc
      721 gtgttcacta ggaaaactaa ttcgtcccca ctgttcaatt gaaccatctg gcatttttcg
      781 ccagcctgaa cctgatgcat atgatgacat atcaggtatc tgattttccc cagttcccac
      841 attccgtttt gccgcttctc ccaaaccaac gtttatgaag atgcagaaat aacgagcaaa
      901 tggcatcatt cctgcttttg tcagggggag ctaccatgct tattggctat gtacgcgtat
      961 caacaaatga ccagaacacc gatctacaac gtaatgcgtt gagctgtgca ggatgcgagc
     1021 tgatttttga agacaagata agcggtacaa agtccgaaac ctagggctga cactgctgtt
     1081 cttgttgcaa attaaaggat ttaatagtga gtagcggcct tactaatgta aagccgctat
     1141 aatcatttta ttaattgcat taatcgtgtt ttggccatac tttcaatgca atttccccca
     1201 actttttacc ccgctccagg atttcatcct catcccattt atccttaact ataagtggaa
     1261 tgttcagtcg tagattggtg tggacgatga gagcatcacg ttttttcaga aatacagcat
     1321 tctgaacaga acggtttacg ctaaggttaa gcaaagttag atttcccaac gtagctatcg
     1381 cttgttgccg tttccttacc agtagctgtt caggggtaag atcggttcca gacagaacaa
     1441 tttggttcaa taccgtagca tctgaatttg tcaccatatg accattttcg agaggccaac
     1501 aggaatacca actttgaggc ataagatgat cgatatcgag gttagaaaga tttggaacat
     1561 caggcttctc tgtcttcact tggcgacaaa gttctctttc aagttccgtt aacattgagc
     1621 gcattttcgg tgcgtcgagc ctgccaggat aaagtggagc attgatgcaa gcgttgagaa
     1681 attctgagtc cccaggccaa cgtgaggctt cgccatttaa gctattgagg atattacgta
     1741 actcaacact ggaaatttcc gttttagaca agtgccgcaa tacattcata aatacattgt
     1801 tgtaattctt tggcgtcagg ccacatacgg ctcttcgtac tacgtaggag acaagatcat
     1861 tatacatggc tgctttctca tcatcggcga tgttagctat cgaaatgaac aaagcaagcg
     1921 gataaagtgt cgtcacatca taggctgcga tgcgatgtcc aaagtgtgag atgggggttg
     1981 tgccaaaacc accaaccaat tctttatatt gtgatgcata ttgtttgagg cgctttactt
     2041 gcagatctgc tcgttgtgaa ggcaagtcct tacttacata atcacgatac tcattgtaaa
     2101 gacgagacag atcgatttca cgttgccttt ctgattgcaa tgtcgcatgc actagccact
     2161 ccatgcgtgg tttattaata cgaccacgac gttgtctttc tgaccagtac gtatcttcaa
     2221 agatcttcca ctcattctca tataattcaa cagcattaat attttcatgt tcagcgcaca
     2281 taaaaatata attgcgaata agatctgtgg catgaagttc cgcacctctg ccatttaatg
     2341 tttcaaaaat tatttgagcg tcatcttcag cctcgagaaa tatgcttact agtttcagat
     2401 ccgtcaagac agcctcaatt aatgctacag cattttctcg tggtgagtga ttttctattt
     2461 taatccactt tataaaggct tcagtaaaaa aacataatgc ttctagtgat ggcgggtgat
     2521 taaaatgctt acgcaacgta ccatgctgcg tgaaactatc agagaataca ttccggatat
     2581 cgtcaatatt ttcaacatta aaactttgaa taaaatgagt ttgatcccga aaagttggcc
     2641 acagtttgaa gcgctctact tctttatttc gcattgtgtc ttcgtttgag tttttcaggc
     2701 aagacgaaat gagaggccct aagttggaaa gatctgtcgc acgtaatgct aatcggatgg
     2761 atgccagaac atattgaaga gtggtcaatc gttgctgacc atcaataata tgtatggaat
     2821 ctacacctaa taatcctttt tttggttgag gttccagtac cactgcacca aggaaatggg
     2881 gagttggttt tgtacccgag agccgggatt gtgctttctc taggatatcc tccagtagag
     2941 ctgaccattg gtttcgctga gtccatacat aggcacgttg atagaatgga acgcagtatt
     3001 gtcggcggtc ttgaaaaagt tgttggacag ttagcgtctc ggatttcatg gtatacctgt
     3061 aagcgacatt gtacaaaagt gaggagttaa tctgatttag aacaatcatt ttatgttgct
     3121 atgatacaac gtatttgtta ccggttaagt aaaaaacgcg tctgaacgct gacgttttag
     3181 ggctttacta gttattcttt cttatctctt catcatccca tcggcatcct gtcgaggttg
     3241 ttgacttgtt gttatttatt ttgtttaggc gctgtttaat tattattgat gggactggtg
     3301 gtgagaggtg attgaaaccg cagacaggtc gtatgcaaga cgtgctgcgg ttggctggta
     3361 aatgttagat agtgcgagta ttgaatgatt tctagccgtt attgatttta cgtatttttg
     3421 tatgagagga tttgcatctc ctgccaccga ccctccatga ctttacgcca ctgtctctag
     3481 ggctgctatg tgccaggagc agccattgct aagtccatcc tgtattgtgc aggtcagctc
     3541 gtttttaaag agtccggcca ccatcttact ggtacagaca ccatatactt tgtgacggtc
     3601 aggctacata tgcacaactc aacttattca tctatttttt gctttagcat gtcagtgttg
     3661 ctttctcgtc ggcgggtgag cggtgacctg acctgtcgat aaaggaacgt agcacgtttt
     3721 atgcaacacc cgcatgcggc agaaaattat tgccgaacgt ttacccctgt caacaagctt
     3781 tactttctga ggcgcgccag cccgcgagga aaacaatctg aacatcaaac aattaatgac
     3841 acaagaaata cgattaaaga tttttttgtg catgccgata gtgctttttt aaaaggagaa
     3901 atctatgtct gtcacaattc agggaaatac ctcaaccgtt atttcaaaca actccgcccc
     3961 ggaaggaaca tcagaaatag ccaaaatcac aagacaaatt caggtgctga ctgaaaagct
     4021 tgggaaaatc tcatcggaag aggggatgac gacacagcag aaaaaagaaa tggctgcatt
     4081 ggtacagaag caaattgaaa gcctcagggc tcaactggag cagttgttaa ggcagcaggc
     4141 agagaaaaag aatgaagacg cgacagttca tcctgataaa aaagaagaga aaaaagacga
     4201 tacaaatacc gctggcacca ttgatattta cgtctaagtg acagccgtat tgtgtgatct
     4261 tacccagcaa tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca
     4321 tgacaaaaac agtatcaacc agtaaaaaac cccgtaaaca gcattcgcct gaatttcgca
     4381 gtgaagccct gaagcttgct gaacgcatcg gtgttactgc cgcagcccgt gaactcagcc
     4441 tgtatgaatc aca
//
LOCUS       DACAPB010000267         4435 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.271, whole genome shotgun sequence.
ACCESSION   DACAPB010000267 DACAPB010000000
VERSION     DACAPB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.271"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            382..1500
                     /locus_tag="IT904_004440"
     CDS             382..1500
                     /locus_tag="IT904_004440"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP3194121.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1566..1814
                     /locus_tag="IT904_004441"
     CDS             1566..1814
                     /locus_tag="IT904_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAP3194122.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1879..2247
                     /locus_tag="IT904_004442"
     CDS             1879..2247
                     /locus_tag="IT904_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP3194123.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2341..2994
                     /gene="nfsB"
                     /locus_tag="IT904_004443"
     CDS             2341..2994
                     /gene="nfsB"
                     /locus_tag="IT904_004443"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAP3194124.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3102..4349
                     /locus_tag="IT904_004444"
     CDS             3102..4349
                     /locus_tag="IT904_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP3194125.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
BASE COUNT         1076 a         1083 c         1076 g         1200 t
ORIGIN      
        1 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
       61 aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatagctg tttatttata
      121 cagcgagtcc tgtttcggac ttctcactcc tcatttaatc aactgaagct gaacccgttt
      181 ttttctgcat gactcaatcg tcaaaattct tcgccaccag tcagcactgc cataacttgt
      241 cacattgcaa aagtagccaa attgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      301 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      361 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
      421 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      481 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      541 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      601 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccacagacca tcatctggaa
      661 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
      721 gaacgctatc aacgcacgct ggaaaacttt ggttatctca ttcagcaggc gaccgttttt
      781 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
      841 ttgtcacgat ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaggga
      901 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
      961 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tatttcgctg tttgagttac
     1021 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     1081 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg
     1141 gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt caaacatcag
     1201 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     1261 ggcgtcatta ccgatccgca cactggtgat cgtcgaccgc taacggaaga taccttgcga
     1321 ttgctggaaa aaatcgcccc ttccgcacat aaaattggcg catcgagcgc aattgaagca
     1381 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     1441 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa
     1501 gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1561 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1621 tcctctcttg cctgctgctt cccgcccccg cattggggct gacgctggca caaaaactgg
     1681 tgagcacgtt ccatctgatg gatcttagtc agctttacac tttattgttt tgtctgtggt
     1741 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     1801 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     1861 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     1921 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     1981 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     2041 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     2101 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg
     2161 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2221 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     2281 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     2341 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagtaaa
     2401 aaacttaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc
     2461 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2521 gccaaatccg ctgccggtaa ttatgtgttc aacgaacgta aaatacttga tgcctcgcac
     2581 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     2641 caggaagatg ctgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     2701 cgcaagttct tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     2761 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     2821 gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag
     2881 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     2941 gctacgctgc cgaaatcacg tctgccgcaa aacatcacct taaccgaagt gtaattctct
     3001 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     3061 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     3121 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     3181 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     3241 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     3301 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     3361 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     3421 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     3481 agaaattccc ggcagcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     3541 tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     3601 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     3661 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     3721 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     3781 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     3841 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     3901 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     3961 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     4021 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     4081 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgactt
     4141 taatggtacg ccaactggct ccaggtgata atggtttacc gctcgagatc tatgcgttta
     4201 ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     4261 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     4321 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     4381 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgtt
//
LOCUS       DACAPB010000268         4432 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.272, whole genome shotgun sequence.
ACCESSION   DACAPB010000268 DACAPB010000000
VERSION     DACAPB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.272"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..388)
                     /gene="pdeB"
                     /locus_tag="IT904_004445"
     CDS             complement(<1..388)
                     /gene="pdeB"
                     /locus_tag="IT904_004445"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAP3194126.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVAYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYR"
     gene            complement(552..1022)
                     /locus_tag="IT904_004446"
     CDS             complement(552..1022)
                     /locus_tag="IT904_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194127.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(1138..1689)
                     /gene="maa"
                     /locus_tag="IT904_004447"
     CDS             complement(1138..1689)
                     /gene="maa"
                     /locus_tag="IT904_004447"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAP3194128.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(1861..2079)
                     /gene="hha"
                     /locus_tag="IT904_004448"
     CDS             complement(1861..2079)
                     /gene="hha"
                     /locus_tag="IT904_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAP3194129.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(2105..2479)
                     /gene="tomB"
                     /locus_tag="IT904_004449"
     CDS             complement(2105..2479)
                     /gene="tomB"
                     /locus_tag="IT904_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAP3194130.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(3025..>4432)
                     /locus_tag="IT904_004450"
     CDS             complement(3025..>4432)
                     /locus_tag="IT904_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP3194131.1"
                     /translation="ATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNTG
                     IAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDFE
                     LIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALGV
                     SINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQMV
                     PFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVGY
                     DWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALLA
                     ATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRLR
                     PILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVRR
                     RFSRKNEDIEHSHTVDHH"
BASE COUNT         1213 a         1060 c         1024 g         1135 t
ORIGIN      
        1 cacgataacc atctttcgaa attttacctg gctcggggaa ggtatcgggc ggactttcat
       61 gctccagaga cgaacactgg ggaacgttgt tatcgatata agccacttcc tgaatatagc
      121 gataactgta agatacccga cgcatttcca tgagatgggc ttcgctacag gcagcgcctt
      181 gccatctttc cagctcctgc agcgcatctt tcccttgtgt cgccacctta ttggctcgaa
      241 tagcgacgcg ggaggaatag gtatccagct cttcaataaa cgatgtttct acctgctgat
      301 gggccagcca gatgcttaag ccgacaggca gcaatactga aagaatcagt actcccgaaa
      361 taaggccgac cagatgtcgt gttctcacca gcatatcctt attaacacag tgtagggaaa
      421 gtatatctga ttgtggaaaa aggctggctg gagataagga ggtagagctg gtatttcatg
      481 aagtgatggg tggaaacgat cattgtcatt tgaccaggcg cagagcttta cgcctggtat
      541 gtaaccctaa cttaagatgt tgattcggcg cgagcgaggg taaaaatatc gtaaaaagac
      601 agttcacctt tacggacgat cattttttgc agttgttcct tatgaacatc cgcgacacgc
      661 ggagagttca agatggcttc aaccagcgcg gcaggtacaa agcgcgtgtt gtaccattcg
      721 ccgttatagc aaacgcggaa atccagcacg tcgatatctt catagcggta gcgtgggtag
      781 acatcgaaaa agaagaaacc attaagcagg ataaataata caaccagtag ccagacagag
      841 cctgacagcg tttcggactg caacatcacc gccagggtgg caaaaaaagc aacgtacata
      901 ccgataaaca gccccggatg tttacggata aaactgatac taaagcgggg tttgttgtcg
      961 cgtttttcgc gggtattcag agactcaatc gtttcggtca gcaggcgttg tatttcggtc
     1021 atcgtttgcc ttcgtggtat ctgcaacttt ccagggaaca ccctatttta acgtgggaat
     1081 agtagagggg aagtaacaga tctaccacaa ttttgcataa cagttgcgaa aaaccgatta
     1141 caatttttta attattctgg ctggattacc gcccacgaca acgttgtccg ggacatcttt
     1201 tgtgacaact gcacctgagg ctaccacgac gttatcacca atggtcacac cagggttaat
     1261 gaccgcgcgt ccgccaatcc agacgttatt accgatggtg acgggtttcc ccagttcagc
     1321 accgctatta cgtgctacag ggtcgatggg atgtgttgcc gtgtagatat gaacgcctgg
     1381 tgccaacata cagttatcac cgatgcgaat agggcagaca tcaagcatca cgcaatcgaa
     1441 gttggcgaaa aaattattac cgagaaaaat gttatagcca tagtcacagc gaaacgttgg
     1501 ctcaatataa gcctctgtca cctgaccgaa tagatcagcg agaatttgct ggcgtaatgt
     1561 gtgctcttcc gccagggaat gattgtatcg gtgaataagc tgacgagcgc gcaggcgatc
     1621 gcgagataac gtctcatctg ccgagcgata caactcacca gcaatcatct tttctttttc
     1681 tgtgctcatt actgacctcc ggttgctgaa aatgcggcaa cacagtaaga cgaaaagtca
     1741 gtgaagagaa cgttcccatt acatctgtgt gataaagatc acatagggta tgttttcatg
     1801 gcgaaaaagt ataaaattct taataaacag ccggttatag ctccgaaagc gaattattta
     1861 ttagcgaata aatttccaaa ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa
     1921 ttcggcgagg cggtgatctg cggctgagta aaataccgcc agttcattat ctgataattc
     1981 gtatttattt ttctcgataa cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg
     2041 cattaaataa tcggttttcg ttaaaggttt ttcggacata cttctaccca tggttgtaat
     2101 aattctaaca agataaactc gcaggattct ctttcgtcgc attgacaaaa caacggaata
     2161 gtcgattacc tgacttccgc catttctgaa gatcctgcat attaatacca taactactga
     2221 acaacataaa ggtgtcatcc agatattcgt cgatctgctc aatgagctta ttgtcttcat
     2281 tatacttaat tttgtaatta agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact
     2341 ggaggttgat cgccgaggtt gggtcgttaa cccagccatg attgctttct tcaaggtttg
     2401 caaggcagtc atgatacagg gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt
     2461 tgggtgagta ttcatccata acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt
     2521 cgggatggaa aaaacttact gacctggact tgccctctcg tttgttagct taactataag
     2581 ccactctttg caggttttca tcgcattatt acgaaaaatt acaattagga aggaagcggg
     2641 acaggactca ctttttcata ctagcaccaa cccgcagcag gtaaaagcag tttgctcgaa
     2701 cagtttataa catttcagga ttatcgccag atgacattgg tgacactaat accaggattg
     2761 ctctgaatat gacgtaataa ccgaggaatg aataaagaat taccgcaaat aattaagaat
     2821 agcctctaaa tgattatgga ttattaaatc tattagcgct acttaatata aattaactaa
     2881 caatcagtat tttttatgaa ttttatccgt ggttaatact ggttttcgta tgagatcctg
     2941 agttggtggt tcaattactc cttaatgttc gtaggttatg cataaaaaag gccgcttgcg
     3001 cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc
     3061 ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat
     3121 tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc
     3181 gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa acgccagcga
     3241 ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt caatcagacc
     3301 tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc
     3361 cgacaaccca atggttgtga gtaggcctac ctggaagtaa acgtcattgg tcaggccacg
     3421 gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa
     3481 cggaatcgac cagctctcgt acagcgccgc cagacacagg aacacgacaa tcaacgaaat
     3541 cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca tccccgtcca
     3601 gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc
     3661 accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca ggccgttgta
     3721 acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg agaacggcac
     3781 catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta
     3841 tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac
     3901 atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag aaacacccag
     3961 cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt
     4021 tggacgtacg ctggtcagca tatcagggtg cttcgctgct tctgcaagca gctggttacg
     4081 cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc
     4141 ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt
     4201 gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc
     4261 cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct gaccacgtcc
     4321 cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt ctttggtcag
     4381 atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg ca
//
LOCUS       DACAPB010000269         4428 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.273, whole genome shotgun sequence.
ACCESSION   DACAPB010000269 DACAPB010000000
VERSION     DACAPB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.273"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(16..606)
                     /gene="caiE"
                     /locus_tag="IT904_004451"
     CDS             complement(16..606)
                     /gene="caiE"
                     /locus_tag="IT904_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAP3194132.1"
                     /translation="MSYYAFEGLIPIVHPTAFVHPSAVLIGDVIVGAGVYIGLLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLIGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(612..1397)
                     /gene="caiD"
                     /locus_tag="IT904_004452"
     CDS             complement(612..1397)
                     /gene="caiD"
                     /locus_tag="IT904_004452"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAP3194133.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(1506..3059)
                     /gene="caiC"
                     /locus_tag="IT904_004453"
     CDS             complement(1506..3059)
                     /gene="caiC"
                     /locus_tag="IT904_004453"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAP3194134.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGICKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLCEESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRVGFCYEAEIRDDHNRPLPAGEIGEICIKGIPGKTIFKEYFLNPQATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(3133..4350)
                     /gene="caiB"
                     /locus_tag="IT904_004454"
     CDS             complement(3133..4350)
                     /gene="caiB"
                     /locus_tag="IT904_004454"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAP3194135.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
BASE COUNT         1013 a         1224 c         1088 g         1103 t
ORIGIN      
        1 ccgcatccgg caagatcacc gtttcggcgt cacatccgtc gtcccctgca aacgggggcg
       61 attttcctcc atttgcctca acggctgcgt ttcatgtaac gatgcatgac agcgcccgac
      121 aagatcctga tactctttgg tattcaaccg tttccagtgt aactcgtcgt cactaacact
      181 gcgtacagcg cgggctggag tacctatcaa caactggcgt ttctcgccgc taaagcctgc
      241 tttgacaaag ctcatggcgg caacaatgct ctcttcgcca atgaccgcgc catccataat
      301 cacgctgttc atcccgacca atgcatcgcg accaatcaca caaccatgca ggatcgctcc
      361 gtgcccgata tggccgtttt ccccaacgat ggtgtcagtg tcgcagtagc catgcataat
      421 gcagccatcc tgaatattgg ctcccgcttg cacgatcaac cgcccgtagt caccacgcag
      481 tgaggcgagt aggccgatgt agacaccggc tcccacaatc acatcgccaa tcaatactgc
      541 actgggatgg acaaacgccg tcgggtgaac tatcggaatt aacccctcaa aggcgtaata
      601 gctcacggtt gttaacgtcc tttccacacc ggatcgcgct tctcggcaaa cgccagcggc
      661 ccttctatgg catcttccga atgcagaact gatggatagt gtttcaacac gccgctgcga
      721 atatagcgat acgcttcttc taccggcatt tcgctggtgg tgcggtagat ctctttcagc
      781 gccgcaatcg ccagcggagc gctgttaacc agctgctgcg ccagttcgcg ggcgttatcc
      841 atcagttccg cctggctaac cacgcggttg actatccccc aacgcagcgc ctcttctgcg
      901 cccattcgtc tgccggtcat caccatttca ttgacgatgg caggcggcag gatcttcggc
      961 agacgcagca cgccgccgct gtcaggaacg atacccagtt tggcttccgg cagggcgaag
     1021 ctggcgttat cggcacaaac aataaaatct gccgccagcg ccagttcaaa gccgccgcca
     1081 aaggcatagc cgttcacagc tgcgataacc ggtttgtcga gattgaaaat ttcggttaat
     1141 cccgcaaaac cacccggacc aaagtcagca tccggtgctt cgccttctgc tgccgctttt
     1201 aaatcccagc ccgcggaaaa gaacttctct ccggcaccgg taataatggc gacacgtaat
     1261 tgcggatcgt cacggaaatt tagaaatact tcgcccattt caaagctggt ttttgcatca
     1321 atagcattcg cttttggacg atcaagggta atttccagaa ttgatccatt gcgggtcaga
     1381 tgtaatgatt cactcattcc ttttctccat ttttgctttt tcagggacga caacatccct
     1441 gcaaaaaatg catattgttt tagagtgtga ttattagctg gcagggtagt tccctgctgt
     1501 ttcatttatt tcagattctt tctaattatt ttccccgagc aattacgtgg cagatctttt
     1561 ctgatctcca gataagaggg cactttaaat ttcgccatat tttgttcgca gaagcggaaa
     1621 aattcctctt cgctcaatgt ttcaccttca ttcagcacca caaatgcttt gatggcttca
     1681 tcgcgaatcg aatctttaat acccacaacc acgatgtcct gaattttcgg gtgcgcggcg
     1741 ataatatttt ccagctccac gcaggagaca ttctcgccgc cacgtttaat catattgcag
     1801 cggcgatcga cgaaataaaa aaagccctct tcgtcgcggt atccggtatc gccggtatgc
     1861 agccagccat cggcttccag cactttcgca gtggcttgtg ggttgagaaa gtactctttg
     1921 aagatggttt tcccaggtat gcctttaatg cagatttcac cgatctcacc agccgggagc
     1981 gggcgattgt gatcgtcgcg gatctccgct tcgtagcaaa accccacccg accaatcgac
     2041 ggccagcgtc gtttatcgcc aggacgatcg ccgataatgc ccacaatggt ttccgtcatc
     2101 ccataagacg tcagcaagcg aacgccgaag cgttcacaaa acgcatcttt ttcctgctcc
     2161 gacaagttga gataaaacat cacttcccgc aggcggtgtt gctgatcgtt cgctgaaggc
     2221 ggctgtacca tcaacgtacg gatcatcatc ggaatacatt cggtaacggt ggcgcggtac
     2281 ttctgtacct gtccccagaa ggcgcgggcg ctgtatttct cgaccagcac aaaggtggcc
     2341 ccggcagaaa acgccgccat cgccgcagta cactggcaat cgatatgaaa cgcaggcatt
     2401 accgtcaggt agacgtcatc gtcacgcagt gcacactgcc aggcggagta atatccagcg
     2461 aagcgcaggt tgtaatgggt aatcaccaca cctttcggtc gggaggtggt gccggaggtg
     2521 aagagaattt ccgccgtatc gtcagtcgat agcggcggtg catagcacaa ggtggcaggt
     2581 tgttgatttt tcagttgagt aaacgaactc acgccatcat cagcgggaag tgccacatct
     2641 gtcaggcaaa tgtgccgcaa ttgagtggca tcttcctgct gaatctgttg atacatagga
     2701 tagaattgcg cactggtcac cagcaggcac gcctggctat tttgcaggat ccacgcgctt
     2761 tcctcgcaca acaggcgggc gttaatcggc accataatcg cgccaatttt tgccagcccg
     2821 aaccagcaaa agataaattc cgggcagttg tcgagatgta gtgcaacctt gtcgcctttg
     2881 caaatcccca gcgtataaaa caggtttgcc gtgcggttaa tctcctgatt taactcaaga
     2941 taactatacc ggttaacgac tccgccgctg gattcacaaa tcagcgccgt tttatgaccg
     3001 taaacgtccg caagatcgtc ccacatttga cgtagatgtt gtccgccaat gatatccatt
     3061 gcacctctat ccatttttgt tcgtttgtta ttgggcgggc gctagtcagg caagccgact
     3121 gacgccacgc gtttagtcct caactttggc cagacctttg ctgaccaact cctgaatgtc
     3181 gttttcgctg tagccgatat ttttcaaaat ggcagccgtg tccatgccat gtgagggcat
     3241 tccgcgccag atttgtccgg ggttattttt gaatttcggc atgatgttcg gccctttgca
     3301 ggtgcgacca tccatcgttt gccactgagt gattgattcg cgagccacat actgtggatt
     3361 gctttccagt tccggtacgg tcagcacttt ggcgcaggcg atattcagtt cagcaaagcg
     3421 ttcttttact tccgcgatgg tatgtgtcgc cagccaggca tcgagtttct cttcaaccag
     3481 tgggccgtaa gggcattcga tacggtggat aagctgagtg ccttccggga tttctggcgt
     3541 gccaagcaga tgtgcgaggc caatatcttt aaagcactct tcaatttggg taatgcccac
     3601 cagttccatc acgatgtagc cgtcggcaca tttatacaga ccgcaaccgg cgtagtaggg
     3661 atctttacct ttgctcatgc gcgggcacat ttcgccgccg ttgaagtaat ccatcatgaa
     3721 gtactggccc atacgcagca tcacttcata catggcgatg tcgatacttt cgcctttacc
     3781 ggtttcacgc actttatgca gtgctgccag cgccgccgtg gtggcggtca ggccagaaaa
     3841 gtaatcggcg gtatacggga aggcaggcat tggctggtca acatcaccgt tctgaatcag
     3901 gtaaccacta aaggcctggg cgatagtgtt ataggccgga agattggtgt actcctcggt
     3961 gccgtactga ccaaaaccgg acaggtgagc gataaccagt ttcgggttgt gctgccacag
     4021 tacttcatcg gtaatgccac gacgggcaaa ggccggacct ttactggctt cgatgaagat
     4081 atcggtggtt tccattaatt tcagaaacgc ttcgcggcct tcatctttga aaatatttaa
     4141 cgacagcgcg tgcaaattgc ggcgggagag ttgcgggtag ttcggttgaa cgcgaatggt
     4201 gtcggcccag gcgacgttct cgatccagat aacttccgcg ccccattctg cgaacatttg
     4261 cccggcaaac ggtccggcga tttcgatacc ggagaagaca acgcgcaatc cggccaacgg
     4321 cccgaatttc ggcatgggta gatgatccat tatttgctcc tgaaaaattt atgtagcgca
     4381 tgactgccgg atgcggcgta aacgctttat ccggcctaca ttcgtgct
//
LOCUS       DACAPB010000270         4428 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.274, whole genome shotgun sequence.
ACCESSION   DACAPB010000270 DACAPB010000000
VERSION     DACAPB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.274"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            22..927
                     /locus_tag="IT904_004455"
                     /pseudo
     CDS             22..927
                     /locus_tag="IT904_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            1223..1339
                     /locus_tag="IT904_004456"
                     /pseudo
     CDS             1223..1339
                     /locus_tag="IT904_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
     gene            1341..1474
                     /locus_tag="IT904_004457"
                     /pseudo
     CDS             1341..1474
                     /locus_tag="IT904_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
     gene            complement(1549..3000)
                     /gene="nhaC"
                     /locus_tag="IT904_004458"
     CDS             complement(1549..3000)
                     /gene="nhaC"
                     /locus_tag="IT904_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP3194136.1"
                     /translation="MNNNNTPRSPTLVESLLPILAMILLLGFGYAAFDLPPEPLMVLS
                     TVIAALLVKRLGYGYNAILESISQKIAKTMPALLILISVGLLIGTWMIGGTIPLMIYY
                     GLNLINPSMIYVTALLVTAVVSVCTGTSWGSAGTVGVAFMGVAIGMEANLAATAGAIV
                     AGAYFGDKLSPLSGDTNLAAMAAQIDLYEHIAHLLYTTLPSLILSAFVMTLYGMNSEL
                     GGSTIPEKIQLITEGLKGIYHFNLLLLLPIAIVLYGSITKKPTIPIMLVSALVAMINA
                     SVIQGFALHDIVKSAVDGFNVSMLTDKDVNPLLGNLLNRGGMNSMMSTLLICFCALSF
                     AGTLALSGALEVIVHNLLKLVHSTGTMILATITCGLTMISVTCNGQISILIPIEMLRS
                     AYIERGLHPKNLARTVEDSATIFEPILPWTAAGAYMAGTLGVATLSYLPWAILCWSGI
                     FFATLWGFTGFGIARLSKEKQQQLMMETINESK"
     gene            complement(2993..4171)
                     /locus_tag="IT904_004459"
     CDS             complement(2993..4171)
                     /locus_tag="IT904_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP3194137.1"
                     /translation="MKVYNFDKVISRDGTYSAKYNKKGRDIIPLSVADMDIPVADFIV
                     SELSVANQKGIYGYTLLSDDWQQVAAQWYQRHYSWKVNPEHIVFCPRVVQAVSLFIQN
                     FTQPGDAIVSLTPAYHPISHAVEVNHRVLLESALLYRDHHYEIDFADLEDKFKMACCF
                     ILISPHNPTGTIWSEDNLRKIASLAEKYNVFIISDDVHADFNFTQKQHQTISSISSYV
                     AQNSFICTSPAKTFNMAGLEVANIVIANDKYREQFKLALIAAGIHNPGYFSVPAFLCA
                     YRHGDSWLAALKDYLVENRSWVQSFCQAHFPDWFVASGGGTYMLWIDYRAMNISEEQL
                     RHWFVSLAGVEMSWGSDFGDEGLGFFRVNIATPRSNLTQAFERIYQSIPFTTGVSLHE
                     "
BASE COUNT         1270 a          976 c         1023 g         1159 t
ORIGIN      
        1 ttgaccaatt gaatctacaa ccgcgctctt gatgtcagac tccctgaaca gttctcgata
       61 atcgggaaac tcagagtgca ttatcctgtg gtcacactct gccatgtgtt cggggttcat
      121 cgcagcagct acagatactg gaaaaaccgt cctgaaaaac cagacggcag acgggctgta
      181 ttacgcagtc aggtacttga gctacatggc atcagccacg gttcggccgg agcaagaagc
      241 atcgccacaa tggcaacccg gaggggctac cagatgggac gctggcttgc tggcaggctc
      301 atgaaagagc tggggctggt cagctgtcag cagccgactc accggtataa gcgtggtggt
      361 catgaacatg ttgctatccc taactacctt gaacggcagt tcgccgtgac cgagtcaaat
      421 caggtgtggt gcggtgatgt gacctatatc tggacgggta agcgctgggc gtacctcgcc
      481 gttgttctcg acctgttcgc aagaaaacca gtgggctggg ccatgtcgtt ctcgccggac
      541 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
      601 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      661 tggcgatacc agatcaggca gagtatgagc cggcgcggaa attgctggga taacagccca
      721 atggaacgct tcttcagaag tctgaagaac gaatggatac cggtggtggg ttacgtaagc
      781 ttcagcgagg cagctcacgc cataacggat tatatcgttg gatattacag cgcactaaga
      841 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      901 tctaactcgg tggccagttt ttgttgacca cttcaatgac tagtctaaaa actagtatta
      961 agactatcac ttaaattgat acaacttttt agagtagtca ttagtgaaca gataattgta
     1021 agttaaccgc tccggaaatc ctggaggcta aactccctga gaaagaggca aacaggatga
     1081 ctaaaaaaac acttttttcc cccgaagtcc gtcaacgggt agttcaatgc tttgtcgaat
     1141 aaacatttac ctgaacgatt aactgcaacg gctatcctta ccgacaatca acgtcaataa
     1201 aaatctgtac tgactcgtca gcactgggtt cgatcagtag gaacatgaat tcaaaggcca
     1261 ccttgctgaa tctcaagact cctccgattc caaagataaa ccccgtctta agttcgctgg
     1321 cctgaaattc ggtgactatt gtctatatcg ggatcaccac ggttgtgtga ttaatcctcg
     1381 tcgatcataa agcgtctatg gatgtactgc ggcgttcctg ctactggtga tcaccctgtg
     1441 gttgtgcaat tccaaacggc tgaagcgaga gtaacagaca tcacgccgca atcgggggcg
     1501 gcgtgtagac gtggttaatt actatcaaat atattaatca gaccgattct atttgctttc
     1561 atttatggtt tccatcatga gctgttgctg cttttctttg gacagtcgag cgataccaaa
     1621 gcctgtgaac ccccacagcg tcgcaaagaa aatgccactc cagcagagaa tagcccacgg
     1681 aagatagctt aatgtagcaa cgccaagtgt acctgccatg taggcacccg cggcagtcca
     1741 gggcagaatt ggttcaaata tggtggcaga gtcttctaca gtccttgcca gattttttgg
     1801 atgtaggccg cgctcaatat aagcacttcg tagcatttca atcggaatca ggattgatat
     1861 ctgaccatta caggtcacgc taatcattgt taaaccacag gtaatagtcg caagaatcat
     1921 ggttcctgtg gaatgaacta atttgagcag attatggacg atgacttcta aagcgccgct
     1981 caaggctaat gttcccgcaa atgaaagggc gcagaaacaa ataagcaacg tactcatcat
     2041 cgagttcatg ccaccgcggt ttaacaaatt acctaatagc ggattaacat ctttatcggt
     2101 aagcattgaa acgttaaatc cgtctactgc acttttaaca atgtcgtgta gtgcgaaccc
     2161 ctgaataacg ctggcgttga tcatggctac cagtgccgaa acaagcataa ttggaatggt
     2221 tggtttcttg gtaatcgaac cgtacaggac aatggcaatg ggtagcaata acaataaatt
     2281 aaaatgataa attcctttta atccttcagt gattagctga attttttcgg gaatggttga
     2341 gccgccgagc tcgctattca tcccatagag cgtcataaca aaggctgaca gtatcaatga
     2401 aggaagggtt gtataaagta aatgagcgat gtgttcatag agatctatct gggctgccat
     2461 tgcggctaag ttagtatcgc cagaaagggg agacagtttg tccccgaagt aagcccctgc
     2521 cacaatagcg cctgcagttg ctgccagatt agcctccatt ccgatagcca cacccataaa
     2581 cgcaacaccg acggtgcctg cggatcccca ggatgtccct gtgcatacag agacgacggc
     2641 agtaacgagc agggccgtca catagatcat ggaaggattt atcagattaa ggccgtaata
     2701 aatcatcagc ggaatagtac cgcctatcat ccatgtccca atcaggaggc ccacgctgat
     2761 caaaattagc agagcaggca ttgttttagc gattttctgc gatattgatt caaggattgc
     2821 attgtagcca taccccaacc gcttaactaa cagtgcagca atgacggttg aaagaaccat
     2881 taatggctca ggtggcagat cgaatgccgc ataaccaaat cccagcaata gtatcatcgc
     2941 tagtattggt aataatgatt ccaccagagt tggggatctt ggagtattgt tattattcat
     3001 gaagggaaac tcctgtagtg aaggggatgc tctgataaat ccgttcaaat gcttgtgtaa
     3061 gattgcttct cggcgtggct atgttcacac ggaaaaaacc aagtccttca tcgccaaaat
     3121 ctgatcccca gctcatctca acacctgcga gcgagacaaa ccaatggcgt agttgctctt
     3181 cgctgatgtt cattgcccgg tagtcgatcc acaacatgta agttccgcca ccagaggcga
     3241 caaaccagtc tggaaaatga gcctggcaga atgattgaac ccagctcctg ttttcgacca
     3301 aataatcttt taatgctgct aaccaagaat cgccatggcg gtaagcacag agaaatgcag
     3361 gaacagagaa ataaccagga ttatggatac ctgctgcaat aagagctaat ttaaactgtt
     3421 cacgatattt atcgttagca ataacaatat ttgcgacttc aagtccggcc atattaaaag
     3481 tttttgctgg agatgtacaa ataaacgagt tttgtgcaac gtatgagctt atagaagaaa
     3541 ttgtttgatg ttgtttttgg gtgaagttga agtctgcatg aacatcatca gaaataataa
     3601 aaacattata tttttctgct aatgaagcta ttttccttag attgtcttct gaccagattg
     3661 ttcctgttgg attatgcggc gatattagaa tgaaacaaca agccatttta aacttatctt
     3721 cgagatccgc aaagtctatt tcatagtggt ggtcacgata aagtaacgca ctttccagca
     3781 atactcgatg gttgacttca actgcatgac taataggatg ataggctggt gtcaggctga
     3841 caatagcatc tcccggttgg gtaaagttct ggataaataa tgagacggcc tgtactaccc
     3901 gtgggcaaaa aacaatgtgt tcagggttga ccttccacga gtaatggcgt tgataccact
     3961 gtgctgccac ttgctgccag tcatcactta ataatgtata accgtagata cctttctgat
     4021 ttgcgacaga taattcagaa actataaaat cagctacagg aatatccata tcagctacag
     4081 acagaggaat gatgtcacgc ccctttttat tatatttagc gctataggtt ccgtcgcgtg
     4141 aaataacttt atcaaaatta taaactttca tatttgttac tcgcccgatt ttatcgttag
     4201 ggtaatgtaa tatttcgagg gaggaagtgt tattctgtat tttgttttcg ttattatgaa
     4261 aatttgcata tgtgatgttt atcacaaaga tgttaaggaa ggtgcgaata agcaggtcat
     4321 gtcttcccaa gctgactcgt tgattaaaat ttcgcggatc tgggccgatt tttttcccgc
     4381 aaacacatcg aatcagccta tttaggctat tttttccacc atttctgg
//
LOCUS       DACAPB010000271         4424 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.275, whole genome shotgun sequence.
ACCESSION   DACAPB010000271 DACAPB010000000
VERSION     DACAPB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.275"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..926
                     /locus_tag="IT904_004460"
     CDS             <1..926
                     /locus_tag="IT904_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP3194138.1"
                     /translation="READLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALL
                     LPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDL
                     MLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQ
                     SAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPAN
                     RRDIAVVVAENVPAADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQD
                     TSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            931..1230
                     /gene="ihfA"
                     /locus_tag="IT904_004461"
     CDS             931..1230
                     /gene="ihfA"
                     /locus_tag="IT904_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAP3194139.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            1331..2311
                     /gene="btuC"
                     /locus_tag="IT904_004462"
     CDS             1331..2311
                     /gene="btuC"
                     /locus_tag="IT904_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAP3194140.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWTLGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            2374..2925
                     /gene="btuE"
                     /locus_tag="IT904_004463"
     CDS             2374..2925
                     /gene="btuE"
                     /locus_tag="IT904_004463"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAP3194141.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            2925..3674
                     /gene="btuD"
                     /locus_tag="IT904_004464"
     CDS             2925..3674
                     /gene="btuD"
                     /locus_tag="IT904_004464"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAP3194142.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALYRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            3752..4216
                     /locus_tag="IT904_004465"
     CDS             3752..4216
                     /locus_tag="IT904_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP3194143.1"
                     /translation="MRFCLILITALFLVGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         1061 a         1037 c         1226 g         1100 t
ORIGIN      
        1 accgtgaagc tgacctgtcg ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc
       61 aggaagtgat cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg
      121 ttgaagcctt actgctgcca agcccgatct ctgttgaaat gtcagcaatg cgtctttctc
      181 tgtggactgg cctgctggca accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc
      241 gcattttcga aagcggtctg cgtttcgtac cagatactca ggcaccgttg ggcattcgtc
      301 aggatctgat gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg
      361 caaaagagac cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga
      421 ccggtaaact gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc
      481 aatccgcagc gatttatctg aaaggtgaac gtattggttt tgttggggtt gttcatcctg
      541 aactggaacg taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca
      601 agctcgcaga ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc
      661 gtgacatcgc ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta
      721 agaaagttgg cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg
      781 gtgttgcgga ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta
      841 cactcgaaga agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc
      901 gattccaggc atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa
      961 tatctgtttg ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt
     1021 ttcgaagaga tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt
     1081 aacttcgatc tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat
     1141 attcccatta cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg
     1201 gtcgaaaacg cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg
     1261 ccttttttct tttcactgtc gaagagtcac cgtaaaatca acgccatgac acttcagcag
     1321 aacggatacc atgctgacac ttgcccgcca acaacagcga caaaatattc gctggttatt
     1381 atgcctgtca gttttgatgc tgctggcgct tctcttaagc ctttgcgccg gtgaacaatg
     1441 gatctcgcca ggtgactggt ttactcctcg tggcgaactg ttcgtctggc aaattcgcct
     1501 gccacgtacg ctggctgtat tgctggttgg tgcggcgctg gctatatccg gcgctgtaat
     1561 gcaggcgttg tttgaaaatc ctctggcaga acctggacta cttggcgtct ctaacggcgc
     1621 aggcgtgggg cttatcgccg cggtattgct tgggcaaggg caactcccca actggacgct
     1681 agggctgtgt gcgattgctg gcgcgcttat catcacttta atactcttac gtttcgcccg
     1741 tcgtcatctt tcgaccagtc ggttattgct ggctggcgtt gcattaggga ttatctgtag
     1801 cgcactaatg acgtgggcta tctacttttc cacctctgtt gatttacgtc agctgatgta
     1861 ctggatgatg ggcggttttg gcggcgtaga ctggcggcaa agctggctga tgctggcatt
     1921 gatccccgtg ttgttgtgga tctgttgtca gtccaggccg atgaatatgt tagcacttgg
     1981 cgagatctcg gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg tgctggtggc
     2041 cgcgaccggc tggatggttg gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg
     2101 tctggtgatc ccacatattc ttcggttgtg tggtttaacc gatcatcgcg tattacttcc
     2161 cggctgcgcg ctggcagggg cgagcgcatt gctgctggcc gatattgtag cgcgcctggc
     2221 attagctgcc gcagagctgc ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt
     2281 tatctggtta ttgttaaaag caagacgtta gccgcaaaaa gacggtctat gattaaaagc
     2341 taagattttt actgaccaca cccaggagaa acgatgcaag attccattct gacgaccgta
     2401 gtgaaagata tcgacggtga agtgaccacg ctggagaagt acgccggtaa tgtgctgttg
     2461 attgtcaatg tcgcctcaaa gtgtggctta acgccgcaat atgagcagtt ggagaatatt
     2521 cagaaagcct gggccgatcg aggttttgtg gtgctgggat tcccgtgcaa ccagtttctg
     2581 gaacaagaac cgggcagcga tgaagagatt aaaacttact gtaccaccac atggggggtg
     2641 acgttcccga tgttcagtaa gattgaagtt aatggcgaag gacgccatcc gctgtatcaa
     2701 aaattgattg ccgcagcgcc gaccgcagtc gcgccggaag agagcggatt ctatgcccgt
     2761 atggtcagca aaggccgtgc accgctgtac ccggatgata ttttatggaa ttttgaaaaa
     2821 ttcctggttg gcagggacgg aaaagtcatc cagcgttttt ccccggatat gacgccggaa
     2881 gatcccattg tgatggaaag cattaaactg gcgttggcaa aataatgtct attgtgatgc
     2941 agttacaaga tgttgcggaa tctacccgcc tggggccgct ttctggcgag gttcgggctg
     3001 gggagatcct gcacctggtg gggccgaatg gcgcgggtaa gagtacctta ctggcgcgaa
     3061 tggccggaat gaccagcggt aagggaagca ttcagttcgc ggggcaacca ctggaagcat
     3121 ggtccgcaac aaaactcgcg ctgtatcgcg cctatctttc acaacagcag acgccgccgt
     3181 ttgcaatgcc ggtctggcac tacctgacac tgcatcagca cgataaaacg cgtaccgaac
     3241 tactgaatga tgtcgcaggg gcgctggctc ttgatgacaa actcggacgt agcaccaatc
     3301 aactttccgg cggtgaatgg caacgcgtac gtcttgctgc ggtggtgttg caaatcacac
     3361 cacaagccaa tcccgcaggc caattgctgc ttcttgatga gccgatgaac agtcttgatg
     3421 ttgcgcaaca aagtgcgtta gacaaaattc tgagcgcgct gtgtcagcaa ggactggcga
     3481 ttgtgatgag cagtcacgat ctcaaccaca cattgcgtca tgcgcatcgg gcgtggttgc
     3541 taaaaggtgg aaaaatgctg gccagtggac gcagggaaga ggtgctcacg ccgccaaatc
     3601 tggcgcaggc ctatgggatg aattttcgcc gtctggatat cgaaggtcac agaatgctga
     3661 tttcgaccat ctgatttcat tagtatcttg aaaatcgggt aattacgacg ctaaattaat
     3721 gcaagaataa aaaacagagg aaccgtacgg aatgcgtttc tgccttattt tgatcacagc
     3781 actgtttctg gtcgggtgta gccaccataa agcaccgccg ccaaatgcca gactttctga
     3841 ttcgattacc gttattgccg gtttgaacga ccagctacaa agctggcatg gcacgccgta
     3901 tcgttatggt ggcatgacgc ggcgcggtgt ggactgttcg ggatttgtgg ttgtaacgat
     3961 gcgcgatcgt ttcgatttgc agctgccccg agaaaccaaa gaacaagcct ctatcggcac
     4021 gcaaattgat aaagacgagt tgctgcctgg tgacctggtc tttttcaaaa cggggtccgg
     4081 acaaaatggt ttgcatgtag gtatttacga taccaacaac caatttatcc acgcctctac
     4141 cagcaaggga gtgatgcgtt cctcacttga taatgtctat tggcagaaaa atttctggca
     4201 ggcgagacga atctagtatg tcgaaaaatg gacggcgatg atacatcgcc gtccatttac
     4261 tcctgaatac agtagtattt tgcagacaaa aattccgtaa atggattatt cgtttataat
     4321 caggataatc taaaaaaagg ccccctgaaa aagtggttaa catggaacaa atgaaagtta
     4381 ctccttctgt aattgttatg tcttatggcc ttatcccttt tagc
//
LOCUS       DACAPB010000272         4401 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.276, whole genome shotgun sequence.
ACCESSION   DACAPB010000272 DACAPB010000000
VERSION     DACAPB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.276"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..137)
                     /locus_tag="IT904_004466"
     CDS             complement(<1..137)
                     /locus_tag="IT904_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'') family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HAP3194144.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     H"
     gene            complement(230..1489)
                     /locus_tag="IT904_004467"
     CDS             complement(230..1489)
                     /locus_tag="IT904_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol efflux MFS transporter CmlA1"
                     /protein_id="HAP3194145.1"
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNAL
                     GTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSA
                     EVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGA
                     LVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLY
                     TLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKW
                     GSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALR
                     GFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVS
                     RVKGSRGQGEHDVVALQSAESTSNPNR"
     gene            complement(1751..2542)
                     /locus_tag="IT904_004468"
     CDS             complement(1751..2542)
                     /locus_tag="IT904_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /protein_id="HAP3194146.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     gene            complement(2600..3208)
                     /locus_tag="IT904_004469"
     CDS             complement(2600..3208)
                     /locus_tag="IT904_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAP3194147.1"
                     /translation="MSLALFDFDGTITTRETMPDFVRRSVSRRRLLVGQLLLAPLVLG
                     YKIGILSGTLIRRVIVRFAYSGIPASVLEAQGRDFAQSYLPNTLRGEAMQRIAWHKAQ
                     GHKVVVVSGGLEAYLEPWCDSHGVELICSSLQQSDGILTGRYEGRQCVLTEKARLVRE
                     RYDLATYPTIYAYGDTPEDLDMLAIATKQYYQWQEVGVAGGR"
     gene            complement(3304..4146)
                     /gene="estX"
                     /locus_tag="IT904_004470"
     CDS             complement(3304..4146)
                     /gene="estX"
                     /locus_tag="IT904_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="HAP3194148.1"
                     /translation="MKEKVVVDKAISLYTESFGDPAHEPIILIMGAMSSAVWWPDEFC
                     SQLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDGYGLEAAHLVGMS
                     LGGFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQRAESLDWSDRD
                     AVVAYQVGAWRINSGTAHAFDAEKIQNIAELNFDRTPNILTTFNHTTLGGGERWLGRL
                     NEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHELHHEDWPRIIQAIK
                     GQTS"
BASE COUNT         1102 a         1260 c         1096 g          943 t
ORIGIN      
        1 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
       61 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
      121 atcaccgctt ccctcatgat gtttaacgcc tgagctcagc cgaccgaaac cgcgtagcgg
      181 ttttgggtcg gctgcagcga tttgttgggc gatagcttgc cacattctct caacgattgg
      241 gatttgatgt actttccgca ctttgtagcg cgaccacatc atgctccccc tggccgcgag
      301 agcccttcac tcgggaaaca caagacagac cgagcacgac tgttgcaagg gtcaaacagt
      361 acacgacaac cggccaagcc gtgttgcgcg gcaacagcga aatgatcaac gttccgatgc
      421 ttcctagcag tacaccgccc aagcagaagt agactgccgt gaccgttcca gcaacatggt
      481 cgaatcctcg aagagcgcca ttgggcgcca cagataccgc tgtggcgaca ccaataccca
      541 ctagccacat tggagcaata aagcctaaca cggactgcaa agcccatatt tcggtgatgg
      601 caagcaatac tgctccagct atcaggcatc ccattcccat tcgcaagaca cttgggctgc
      661 cccacttggg tatcacacgc cccataaaac gagccgtaaa caccatggca attgccactg
      721 tggcgaacag caggctgaag ccaagctgag acacaccttg cctgcccatc attagtccgg
      781 gcgcaatgga gaaaaagacg aagaagctac ccattccagc ggcgtaacac aacgtgtaca
      841 accagaagtt caggcactta acggggagta gcagctgcga ccattgcaag cccgcaactc
      901 gttgcacccg ggtttcaggc cagaatcgcc acgctgctgc agatgcagcg atcatgccca
      961 aacctagaaa cgcaaagata gcccgccacc caagccacat gtcgacgagc gctccgagca
     1021 atgggcctac cgccgggacc atggccagca tggatccgag tatgccgtaa atgacattac
     1081 tttcctcgcg acctgcgtaa atgtcacgta ctgttgcaaa tgtggaaaca aggcacgccg
     1141 aggcaccaca agcctgaaga atccgaagcc ccagaaagac ttcagccgat gacgtaagag
     1201 cgaggcccat tgacgccaca acgtaggcga ggccacctcc cagtagaacg gggcggcgcc
     1261 ccagtcggtc cgatagcggt ccaaacaaga gctgaccggc accaatcatg accaagtacg
     1321 ttgtcagcgt aagctgaatt gtgctcgctg tcgtaccaag cgcgtttggc ataaacggca
     1381 ctgctggcaa gtacatgtcc atgccgagtg atgccaataa atcgaacggt gataacaaca
     1441 acaccgtggc ggcaagggag taccgccaac taaagttttt tgagctcacg aaaccatctc
     1501 cttgaacaaa ggatttggcg gcgctcccgt agagcaaaag gattcatgag aacgccgcaa
     1561 caaccgaaaa atgaaggttg ctgcggctta cttgtctgcg ttcttggaag tgctcatctg
     1621 ctgactatct catgattgaa tttgaatcgt aacaactttc aagcaactct gcaaggagcc
     1681 ttattgtgcg cccaacgccg gagttaagcc gccgcgcgta gcgcggtcgg cttgaacgaa
     1741 ttgttagaca tcatttacca actgacttga tgatctcgcc tttcacaaag cgaataaatt
     1801 cttccaagtg atctgcgcgt gaggccaagt gatcttcttt ttgtcccaga taagcttgct
     1861 tagcttcaag taagacgggc tgatactggg caggtaggcg ttttattgcc cagtcggcag
     1921 cgacatcctt cggcgcgatt ttgccggtta ttgcgctgta ccaaatgcgg gacaacgtaa
     1981 gcactacatt tcgctcatcg ccggcccagt cgggctgcga gttccatagc ttcaaggttt
     2041 ccctcagcgc ctcgaataga tcctgttcag gaaccgggtc aaagaattcc tccgctgccg
     2101 gacctaccaa ggcaacgcta tgttctcttg cttttgtaag caggatagct agatcaatgt
     2161 cgatcatggc tggctcgaag atacccgcaa gaatgtcatt gcgctgccat tctccaaatt
     2221 gcagctcgcg cttagccgga taacgccacg ggatgatgtc gtcatgcacg acaagggtga
     2281 cttctatagc gcggagcgtc tcgctctcgc cagggaaagc cgaagcctcc ataaggtcat
     2341 tgagcaatgc tcgccgcgtc gtttcatcaa gctttacggc cacagtaacc aacaaatcaa
     2401 tatcgctgta tggcttcagg ccgccatcca ctgcggagcc gtacaaatgc acggccagca
     2461 acgttgattc cagatggcgc tcaatgacgc ttagcacctc tgatagttgg ttcgaaattt
     2521 cgatggtcac cgctaccctc atgatgtcta acaccagaat taagccgcgc cgcgaagcgg
     2581 cgtcggcttg aatgacttgt tagcgcccac ctgcgacgcc aacctcttgc cactgatagt
     2641 actgcttagt agcaatggcc agcatatcca ggtcctccgg cgtgtctccg tacgcgtaga
     2701 tggtcggata tgtcgccagg tcgtagcgct cacggaccaa gcgagccttc tccgtcaaga
     2761 cgcactgccg cccttcatag cggcccgtta ggattccgtc cgactgttgc aaggaagaac
     2821 agatgagctc aacgccgtgc gagtcgcacc acggctccaa gtaagcctca agtccgccgg
     2881 aaacaacgac aaccttgtgc ccctgcgctt tgtgccatgc gatgcgctgc attgcctcgc
     2941 cgcggagggt attgggcagg tagctctgag cgaagtcacg accttgggcc tccaggaccg
     3001 aggccgggat gccgctgtaa gcgaagcgaa cgatgacgcg acggatcagg gtgccagaca
     3061 agatcccaat cttataccca agcacgagcg gcgccagaag cagttgtccc acaagcagtc
     3121 gacgtcggct cacggaccgc cgcacaaaat ccggcatcgt ctcgcgagtg gtaattgtgc
     3181 catcgaagtc aaagagtgcg agagacatgc gttgtgggcg ctaacgcctt gctcaccgga
     3241 aaattgcgag cgcagcgagt aatttttccg gtgcagcaac ttgttgtacg gtgcaaccga
     3301 tagctatgac gtttgcccct taatcgcctg gataatcctc ggccagtctt catgatgcaa
     3361 ctcatgtccc gtgccttcga gtgtcagcat ttttgaacca cgaatcgcat ctttcagtgc
     3421 caacccatgc acataaggaa gtacaggatc ctccgtgccg tgaatgatca aagttggtac
     3481 agctatctca tttaatctcc cgagccatct ctcgccacca cctaaagtag tgtggttgaa
     3541 tgttgtcagg atattcggag tgcgatcaaa atttaactca gcgatgtttt gtatcttctc
     3601 agcgtcaaaa gcatgcgcag tacctgagtt gattcgccac gctccgacct gatacgcgac
     3661 gacagcatct ctatcagacc aatccagcga ttccgcccgt tggtgatact caatgatggc
     3721 aggatcaaaa gcgggcatat ccggatctgc atctgcaagc cgttctgaag caatcagcgt
     3781 caagctcttc acacgtttcg gatacttgag agctacaagc tgggaaagaa atccccccaa
     3841 agacatgccg actaaatgag cagcttccag accataacca tcaatgacgc gaaccacatc
     3901 atctgctaat tcttcaacgg aatatggagc ctgacctggc tcatagcttg ttgatttccc
     3961 ggtatcacgg tggtcgtacc ggatcacata gcgacccatt ttggcaagtt gggaacaaaa
     4021 ctcatcaggc caccacaccg cagacgacat tgcccccatg atcagaataa tgggttcatg
     4081 ggccgggtcg ccgaatgatt cggtatagag tgaaatcgct ttatcaacaa ctaccttttc
     4141 tttcatactt aaattgcctt cgagttctac gtacaacttt gttttagggc gactgccctg
     4201 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
     4261 ctgcttggat gctcgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac
     4321 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     4381 catacgctac ttgcattaca g
//
LOCUS       DACAPB010000273         4323 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.277, whole genome shotgun sequence.
ACCESSION   DACAPB010000273 DACAPB010000000
VERSION     DACAPB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.277"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..407
                     /locus_tag="IT904_004471"
     CDS             <1..407
                     /locus_tag="IT904_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HAP3194149.1"
                     /translation="REPEKVALLQKVWRQRLSYRLVRSAEESKIALSSVAETRASLPF
                     ISDELATLISQQGLESALNQPLARILEQVQLALDNAQEKPDVIYLTGGSARSPLIKKA
                     LTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(420..2359)
                     /gene="yegI"
                     /locus_tag="IT904_004472"
                     /pseudo
     CDS             complement(420..2359)
                     /gene="yegI"
                     /locus_tag="IT904_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(2356..3117)
                     /locus_tag="IT904_004473"
     CDS             complement(2356..3117)
                     /locus_tag="IT904_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAP3194150.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQFGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(3114..3773)
                     /locus_tag="IT904_004474"
     CDS             complement(3114..3773)
                     /locus_tag="IT904_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP3194151.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSRLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(3994..4053)
                     /gene="ibsA"
                     /locus_tag="IT904_004475"
     CDS             complement(3994..4053)
                     /gene="ibsA"
                     /locus_tag="IT904_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAP3194152.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         1111 a         1182 c         1057 g          973 t
ORIGIN      
        1 cccgcgaacc ggaaaaagtg gccctgttac agaaagtctg gcgtcagcgt ttaagctatc
       61 gcctggtacg tagcgcagaa gagagcaaaa ttgcgctttc aagcgtagcg gaaacccgcg
      121 cctcactgcc gtttatcagc gatgaactgg ctacgctgat tagccagcaa gggctggaaa
      181 gcgccctcaa ccagccactg gcgcggattc tggaacaggt gcaactggcg ctggataacg
      241 cccaggaaaa accggatgtt atctatctga ccggcggtag cgcccgttca ccgctgatta
      301 aaaaagcgct gacagaacag ttgccgggca ttccgattgc aggtggcgat gactttggct
      361 ccgtcaccgc cgggctggca cgctgggcgg aagtggtgtt tcgttaattg aaaactggct
      421 cagcaacggc ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg acgtagcggt
      481 tccatcaacg gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgca
      541 ccgacagtca gcgccgattc cagccgatgt cgtttggcgg ccattttcgc cattaccgcc
      601 tgtctgtctg ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg ttcacagctc
      661 gctttccagt cgatgaccgc ctgggtcaga tgatcaccaa accctttaac ttgcttaacg
      721 ccacgacggg taacatctgc tgctgtttca ataccaaaag accgtaacgc cgctttacgc
      781 gcagggccag cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc
      841 ttctgccgtt cccttgcagt gtcatgaagt gctgccagag cgagtttctc ttcttccggt
      901 aacccgagaa tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc
      961 aagccgccta cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta
     1021 aatacctcac ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag actgccaata
     1081 atccagatag ccgacagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg
     1141 caaagcaaca gcgataacgc tgacagagcg atctcaatca gaatgaagta ttcgcgccgt
     1201 aacaggccta aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcaattgc
     1261 aatgctggcg gtgctactga cgccatcacc atcgcccaga ctttcgccag cacaaaatca
     1321 ccgatggtgg taatgacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc
     1381 agcgcacacc acgggcagtc agtcaaatga gcggggtaaa catgcattgc cgaaacggta
     1441 catttcttta actgttggcg tagtaaatca agtgccgcta cccacgcttt tgccgtcgga
     1501 cgcccggttg ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcgccc
     1561 ggtaacatcg acagcggaat cgatcgcggc ggcggtttta aaccacgccg ctgattatct
     1621 gacgcgtagg cataacggaa atgggcaata tccgtctcca gcgcattccc cgcatcagag
     1681 ataagcggca caccggaata aggatgccgc ccaccaaaca agacgtgaaa aatcagcaac
     1741 gcaaggccaa aattatcgtg attttctgtg cgttcaaagc cgacaaatga cgacagggtt
     1801 ttagctctgg cggcgtaaaa tgcgacacgc caacttcgca taaatgcaac gtgccattag
     1861 cgttaatctg aaaggagtcg ctatcaatca gtaccacttt gctgtcgcga cctaccataa
     1921 aactgttctg gtttacgtca cccacgacgt gcccgtgctc gtgaaccgta gcaaaagatg
     1981 aagcaatatt gcgcgcaaca tagagtagaa aatcccacgc acaatgaggg taactctggc
     2041 gacgatgtgc cgggctatag atcatatgaa tcggttcttt accagaaact tttggcatca
     2101 taaaaccgat aacttttccg cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat
     2161 aattcaacaa ttgcgcgtca gctgtcgcag ccataaaggc aagtttgtcc tgttttaaag
     2221 cgggtggcgg cgtgtgataa atcttggcga cgctatcgac aaactcctcg atatcataaa
     2281 ccgcgccttc accacctttg cccagttcac ggcctggcgt aacgcactca ccagtagcag
     2341 tatataaagt gggtttcatg gtaaccacag cgccagtgcc aacgttttat catcatccgt
     2401 acgctcgttt accgccgggc tggacaaaaa ctgctttaac aattcaggta ataaatcgag
     2461 ttgttcctgc gttgctgacg ccagtccatt gaaaaacggg gtaaagaaag gcacatgcgg
     2521 agaattatcc agcatattta acgccagtcg ctggatacca tccgtaaatg ctgcaacttt
     2581 atgcacacgc tcagtgctgg tgaaagtttc cagtctggaa acggcatctt catcggtaat
     2641 gaagtgcgtc atattggcat attcacccac catcggcgta agcggcaatt gcagaccgtg
     2701 accaaaatcc accaccacgc cgccatcgcc aaactgcata atcaaggtgc cattagccga
     2761 tgaaatcaat cccagaaacg tgcaggcaaa atcgcgaact gctaattctt tggcttccgc
     2821 ctcggcaaaa agtcgctgac gaacggtcag cacaatatcc gtggcgagga tatcattaag
     2881 ccccaattcc ccaccctgca ctttttgcga catataagcc atcgcttcat tgacggcgag
     2941 catcgcgcct tcgcccccct gcgaaacgct acctgcgcca tcagcaagga acatcaccag
     3001 taatggctgc tggtcattta gccaggcaac ctgcatctgg caggcatcct ggcaaggcaa
     3061 gtccgcgctg atgtgcgacg ttccaacagc ggatgcataa accagacgcc agctcacact
     3121 gacgtccagc cttttggcgc ttccagcaca acttccgttc ccggcgtgga gcgggaaacc
     3181 gaacgcagcg aactggataa ccggctaaac agttcacgga attgtaatcc ctgcagtggc
     3241 aaaggctgac gaacgctgat ttgtgccaga gtcttcatat ccgccccctg aacaccgatg
     3301 gaaaagaagg caaacttctt atcttcttcc ccctgaaaca ctttgttggc ggcagcctgc
     3361 cactcatcgg ttggagcgcc atcggtgatc aggaaaatcc acgggcgata atacgaaata
     3421 ccatttgcac gatactcacg tttccgctcc tctaccatat ccagggcttt ggtaatggcc
     3481 gcacccattg gtgtatcgcc ctgggcgaac aggatgggcg gaaaaaaatt agcggcgctg
     3541 gtaaagggtt gttccacatg caccgggccg aacgtcacaa tgccgagttc cactcttttt
     3601 aaagccagcg aatcggcaag cagctcatcg cgaaaggtaa ccaatccggt gttaagttca
     3661 ttaataggtc tgccactcat cgagccagaa acatccagca gcagaatgca ggggcaacgt
     3721 ggttccgggt tactggcaaa atcgctggtg gcaaacgtga tttgttcgct cataagaaat
     3781 cctttcttag agaattattg aggtccggta tcataccgac tgcaataatg aaataagagc
     3841 aggcaaagca actttcagaa ttcggcccgg cggcgcagaa aattttgcta ttcgcctcgc
     3901 aagaacggag agccgtcgat tgacactaag ggcggagtga cataatttca ggagtgaggg
     3961 ttagggagag gtttccccct ccccctggtg ttcttagtaa gcctggaagc taatcactaa
     4021 gagtatcacc agtatgatga cgtgcttcat cataaccctt tccttattaa aagccctctt
     4081 ctccgggaga gggtttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
     4141 ccctgaatgc agtctatcgc taacgcgtga acaaaatagt ttcctctgtg attattgcgt
     4201 aacgactcgc aaagaatatt tgctggcagg atcgcagact acaaagcctg cggattgaca
     4261 atcttatcgt gaaggcataa tttcaggagt gagggtagag cggggtttcc cccgccctgg
     4321 tag
//
LOCUS       DACAPB010000274         4299 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.278, whole genome shotgun sequence.
ACCESSION   DACAPB010000274 DACAPB010000000
VERSION     DACAPB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.278"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(220..1077)
                     /gene="murI"
                     /locus_tag="IT904_004476"
     CDS             complement(220..1077)
                     /gene="murI"
                     /locus_tag="IT904_004476"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP3194153.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPGAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1022..2866)
                     /gene="btuB"
                     /locus_tag="IT904_004477"
     CDS             complement(1022..2866)
                     /gene="btuB"
                     /locus_tag="IT904_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAP3194154.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2931..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3235..>4299
                     /gene="trmA"
                     /locus_tag="IT904_004478"
     CDS             3235..>4299
                     /gene="trmA"
                     /locus_tag="IT904_004478"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAP3194155.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTH"
BASE COUNT         1098 a         1114 c         1056 g         1031 t
ORIGIN      
        1 gctttttctc agcggcgcgg ggtgtgcata atacgctttc ccgctacata gtcaagcatt
       61 tatttttgct ttctctgccg gagttctcag gagaaccccg ctgacccggc ggcgtgtttg
      121 ccgttgttcc gtgtcagtgg tggcgcatta tagggagtta ttccggcctg acaagaggaa
      181 atttaaaata attttctgac cgcgcaacat tcaaccaaat cagcctaaaa ctgccagttt
      241 ttcgagcgtt tcgaagccgt aacgctgtaa aacgggcaat aattgttcag ctcctggcgt
      301 cattgccata caaaaggcaa tattcgcatc ggcagatttt gcatccgggg cttcatgttc
      361 taacaaccag gccgttcggc gagcaatcgc tgcgccagaa tccaccagcc gggttccctc
      421 tggcagcact tgtaacagtt cttcttgtag tagagggaaa tgggtgcaac ccaatacaac
      481 ggtatctggc ggctctttca ttcttaacca cgggcgtagg atacgtttta gtgcatccag
      541 agaaacatct tcgccatgta gcttcgcttc agccaactca accatctctg ccgagcccag
      601 catttctatc tggcattcat tagcgaaacg cgcgatcagc tcatgagtat aagaacgttt
      661 aactgttccg cgggttgcca gtaatccgac aatgccattt gccgtcagac gtgcagcagg
      721 tttaatcgcc ggcacgacac caacaaccgg gaagtcgaac ttttcgcgta atgcaggaag
      781 tgaaacggta ctggcagtgt tgcaagcgac cacagccagc gcaaggggat aacgttcttg
      841 caccgcggtg acaattgcca ccactcgctc aacaataaac gcttcgcttt tttcgccata
      901 cgggaaagcg acgttatcga aagcataaat gtaatggaga tccggtaaga gatgccggat
      961 ctcgtcatag accgacaacc caccgacgcc ggagtcaaac accagcacgg tgggacgtgg
     1021 ttcagaaggt gtagctgcca gacaaggtgt attcccgtcc tgcagtttgg tagccataga
     1081 ctgtctcata atctttgtcg aacaggttgg ctattttacc acgaactgtc aggtgagagg
     1141 tgaccggata cgcaaccgca agatcccaca agctcacacc gcccattttt acggtttgat
     1201 aaggataaga tgagtaatcc ttatcatagc gagtgcctaa atactgataa gtaatacccc
     1261 agtcgaagtc atacaactgc cagtcgagct ggtatttcac ctgctgttta gcacggcgta
     1321 acaacggcgt gtcggtaatc gcattgcgcg catcgacata atcataactc acagtatgcg
     1381 tcagtggtcc ggtatcaaaa ttggcggtcg cctcgacgcc cttaatccgc gctttccctt
     1441 cgttgtaata tttcagggtg tgatcatcat aatcgatcaa gtcactgaca tcgttacgat
     1501 atccggaaat acgccagttc accccagcgg ttaagccttc aaacgcgcct tcccactgtt
     1561 tgcttttctc cgggtccaga ttcggatttc cgtagaagcc atacagttgc cccagatttg
     1621 gtgccttata agatgtcccg taggaagcaa tgaagcgata accttcgatg aattcccaac
     1681 cggcgctggt ttgccaggtt ccatgacgac caaactgtga gttatcgtcg ctgcgtgctg
     1741 cgccttcaaa ggtaaaatcg ccgacttgtt gcagcccggt cagatagatg ccggtattac
     1801 gttgatcata tccatcctca acataacctg tacccggcgt cgtagtctgt ttctgccagt
     1861 cgacacccgc accaatacta ccgtgaccaa cgatgacatt gtttgcccac tggacggtgt
     1921 attgcttcat ctcatcgagc gtcgccgacg aatcataacg accataatgg ggatcgtagt
     1981 tgtaatcttt gctatggcta tagctggtaa tgagttgtga tttaatcagt tcgccgttat
     2041 agcgcagccc ggcgtcccaa ctttggctat agagtttacg ggtatcgagc aatggtgaac
     2101 cgggagaata atacgcgtca taattggtac ggttatcata gccatagccg cgcacaaagc
     2161 cgctccaggc atcagtaaag ttatgctcca gcgcgccata aagcgtttta cttaaaaaac
     2221 catcgttatc tgtctgcgct tgcgttccgg tattaccata ggcaacaaca tcataaccat
     2281 gagtatgggc ataatcgccc aacagcgtta cccgtgtctt atcccccagt tgttgctgcg
     2341 tagagacatc atagttctga taactattgc ttccccaccc tgctgaaatt tccgttccgg
     2401 gttcatcgcg cgtcgtgatg atattcacca ccccgcctat tgcatcggaa ccataaacag
     2461 cggagcgcgg cccacggata tattcaacac gctggacaag cgcaataggg aactggctaa
     2521 ggtcggcaga accactcacc cccgccagat tcaggcgtac gccatcaatt aacaccaaca
     2581 catgactggc atttgtaccg cgaataaaaa tagatgagag ctgacctgaa ccgccgtttt
     2641 gggtgatatc gacgcccgga agacggcgca gcacatcatt gaccgaggtc gactgccagc
     2701 ggtcgatatc ctgacgggtc acaacggtgg ttggtgcaag cacagtgctg cgcggctgtt
     2761 caaaacggtt agcagtaacg acgagagtat ccgggctggt atcctgtgcc caagcggaaa
     2821 atgccgtgac ggaacacgcc gtcagcagcg aagctttttt aatcattgta aagcatccac
     2881 aatagaagaa ggatgccgca ggtttcatca atattacgcg atgatgagaa ccagatgcga
     2941 cgttggccgg caggtcttcg ggcttggagg ggtatctaag atactaagag atgatgactt
     3001 cccaccggat ggcagtgtcc gcataacgca atcatcgcac ctttccttac cgctgcgcgt
     3061 cagctccaga ttcgcactgg attccctatt aactcacagg accggcaagt ggatgctaca
     3121 ggttgtaaca agttactgtc cagacgtagc tcacaaatag gaattcatca agatctggac
     3181 atctgatgag caatccctac aatcgccgcg tactttaatt tttcaggata catcatgacc
     3241 cccgaacacc ttccaacaga acagtatgaa gcgcagttag ccgaaaaagt ggtacgtttg
     3301 caaagtatga tggcaccgtt ttctgacctg gttccggaag tgtttcgctc gccggtcagt
     3361 cattaccgga tgcgcgcgga gttccgcatc tggcacgatg gcgatgacct gtatcacatc
     3421 attttcgatc aacaaaccaa aagccgcatc cgcgtggata gcttccccgc cgccagtgaa
     3481 cttatcaacc agttgatgac ggcgatgatt gcgggtgtgc gtaataatcc cgttctgcgc
     3541 cacaagttgt tccagattga ttacctcact acgctgagta atcaggcggt ggtttccctg
     3601 ctataccata agaagctgga tgatgagtgg cgtcaggaag cggaggccct gcgcgatgca
     3661 ctgcgcgcgc agaatctgaa tgtgcatctg attggtcggg caacgaaaac caaaatcgag
     3721 ctggatcagg attacatcga tgaacgtctg ccggtcgcag ggaaagagat gatctaccgt
     3781 caggtagaaa acagctttac ccagccgaac gcggcgatga atattcagat gctggaatgg
     3841 gcgctggacg taaccaaagg ctcaaaaggc gatttactgg agctgtactg cggcaacggt
     3901 aacttttcat tagcgctggc gcgcaatttt gatcgggtat tagccaccga aatcgctaag
     3961 ccgtcggttg ctgctgcgca atacaacatc gcagctaacc atattgataa cgtacaaatt
     4021 attcgtatgg cggcagaaga atttactcag gcgatgaatg gtgtgcgcga gtttaaccgc
     4081 ctgcaaggga tcgacttaaa gagttatcag tgcgaaacca tttttgtcga cccaccgcgc
     4141 agcggtctgg acagtgaaac cgagaaaatg gtgcaggcgt atccgcgtat tttgtacatc
     4201 tcctgtaacc cggaaacgtt atgcaagaat ctggaaacat taagccagac gcacaaggtc
     4261 gaacgtctgg ctctgtttga tcagttcccc tacacgcac
//
LOCUS       DACAPB010000275         4293 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.279, whole genome shotgun sequence.
ACCESSION   DACAPB010000275 DACAPB010000000
VERSION     DACAPB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.279"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..360
                     /locus_tag="IT904_004479"
     CDS             <1..360
                     /locus_tag="IT904_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP3194156.1"
                     /translation="PVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGG
                     QRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNV
                     LLKEIRSLGINIELEDE"
     gene            437..>4293
                     /gene="rpoC"
                     /locus_tag="IT904_004480"
     CDS             437..>4293
                     /gene="rpoC"
                     /locus_tag="IT904_004480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP3194157.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRV"
BASE COUNT         1044 a         1084 c         1193 g          972 t
ORIGIN      
        1 ccggtaaccg ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg
       61 cacgcgcgtt ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca
      121 cagttcggtg gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca
      181 gcatacaccc tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg tcgtaccaag
      241 atgtataaaa acatcgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc
      301 aacgtattgt tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa
      361 ttctcgctca aacaggtcac tgctgtcggg ttaaaacccg gcagcggatt gtgctaactc
      421 cgacgggagc aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga
      481 agagtttgat gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt
      541 cggtgaagtt aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg
      601 ccttttctgc gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta
      661 caagcgcctg aaacaccgtg gcgtcatctg tgagaagtgc ggcgttgaag tgacccagac
      721 taaagtacgc cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg
      781 gttcctgaaa tcgctgccgt cccgtatcgg tctgctgctc gatatgccgc tgcgcgatat
      841 cgaacgcgta ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga
      901 acgtcagcag atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga
      961 attcgacgcg aagatgggtg cggaagcaat ccaggctctg ctgaagagca tggatctgga
     1021 gcaagagtgc gaacagttgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa
     1081 aaagctgacc aagcgtatca aactgttgga agcgttcgtt cagtctggta acaaaccaga
     1141 gtggatgatc ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct
     1201 ggatggtggt cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg
     1261 taacaaccgt ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga
     1321 aaaacgtatg ctgcaggaag cggttgacgc cctgctggat aacggtcgtc gcggtcgtgc
     1381 aatcaccggt tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca
     1441 gggtcgtttc cgtcagaacc tactcggtaa gcgtgttgac tactccggtc gttctgtaat
     1501 caccgtaggt ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcactgga
     1561 gctgttcaaa ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa
     1621 agctgcgaag aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt
     1681 tatccgcgaa cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca
     1741 ggcatttgaa ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc
     1801 ggcatataac gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga
     1861 agcccagctg gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa
     1921 cggcgaacca atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg
     1981 tgactgtgtt aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg
     2041 tctgtatcgc tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta
     2101 tgaaaaagat gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg
     2161 ccgtgccatt ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc
     2221 gctgggtaaa aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa
     2281 accgaccgtt atttttgcgg accagatcat gtacaccggc ttcgcctatg cagcgcgttc
     2341 tggtgcatct gttggtatcg atgacatggt catcccggag aagaaacacg aaatcatctc
     2401 cgaggcagaa gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc
     2461 gggcgaacgc tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa
     2521 agcgatgatg gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa
     2581 gcaggtttcc ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc
     2641 acagattcgt cagcttgctg gtatgcgtgg tctgatggcg aagccggatg gctccatcat
     2701 cgaaacgcca atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc
     2761 cacccacggt gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta
     2821 cctgactcgt cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg
     2881 tacccatgaa ggtatcatga tgactccggt tatcgagggt ggtgacgtta aagagccgct
     2941 gcgcgatcgc gtactgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga
     3001 tatcctcgtt ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa
     3061 ctctgtcgac gcggttaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg
     3121 tgcgcactgc tacggtcgtg acctggcgcg tggccacatc atcaacaaag gtgaagcaat
     3181 cggtgttatc gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt
     3241 ccacatcggt ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa
     3301 aggtagcatc aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat
     3361 cacttcccgt aataccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta
     3421 caaagtacct tacggtgcgg tactggcgaa aggcgatggc gaacaggttg ctggcggcga
     3481 aaccgttgca aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt
     3541 acgctttact gacatgatcg acggccagac cattacgcgt cagaccgatg aattgaccgg
     3601 tctgtcttcg ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg
     3661 tccggcactg aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat
     3721 gcctgcgcag tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat
     3781 cagctctggt gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac
     3841 cggtggtctg ccgcgcgttg cggatctgtt cgaagcacgt cgtccgaaag agccggcaat
     3901 cctggctgaa atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct
     3961 ggttatcacc ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca
     4021 gctcaacgtg ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga
     4081 agcgccgcac gacattctgc gtctgcgtgg tgttcatgct gttactcgtt acatcgttaa
     4141 cgaagtacag gacgtatacc gtctgcaggg cgttaagatt aacgataaac acatcgaagt
     4201 tatcgttcgt cagatgctgc gtaaagctac catcgttaac gcgggtagct ccgacttcct
     4261 ggaaggcgaa caggttgaat actctcgcgt caa
//
LOCUS       DACAPB010000276         4281 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.280, whole genome shotgun sequence.
ACCESSION   DACAPB010000276 DACAPB010000000
VERSION     DACAPB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.280"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..308)
                     /locus_tag="IT904_004481"
     CDS             complement(<1..308)
                     /locus_tag="IT904_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194158.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     "
     gene            complement(348..953)
                     /locus_tag="IT904_004482"
     CDS             complement(348..953)
                     /locus_tag="IT904_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAP3194159.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDCGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            1211..1708
                     /locus_tag="IT904_004483"
     CDS             1211..1708
                     /locus_tag="IT904_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP3194160.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGVISEYGGQKLSEATT
                     KRDGY"
     gene            1800..2732
                     /locus_tag="IT904_004484"
     CDS             1800..2732
                     /locus_tag="IT904_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3194161.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2774..3862)
                     /locus_tag="IT904_004485"
     CDS             complement(2774..3862)
                     /locus_tag="IT904_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP3194162.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
BASE COUNT         1125 a         1025 c          957 g         1174 t
ORIGIN      
        1 ttcacagcgg tcatttgcaa ggttggggac agccaacgcc gacaacgact catctctata
       61 tgaagatgtt gaccaaccgt aataccttga tcgtcaaact gaaccttatc accatgccgg
      121 aacagatcat caaagtgagt gagtgccaac cgacaactgt ttggcgcatt gtcgtactct
      181 tcacaaagca gcgtcagcaa ctgctggcga gccgggagtg cgatatttac cgctctggca
      241 aagacctgtt caacctgtcc ttgcccccgc tgcgccagaa aatccgcatc cgcagttagc
      301 gcccacacac accgtctttc tggtgtgttg atagcaagcc tcccggttta tgcacctgcg
      361 gcaatcagta actgcgcaat ctcttcaaag ccccgttccc gcgccagttc cagtggcgtt
      421 ttgccatatt tatcggtcag atgcgggctg gcaccgtgtt ccagcaataa ctgcacaatc
      481 gcctgctgtt taataccaca atcattaagc acaatcgctt ccagtagcgg cgtccagcct
      541 acatggttgg tctggttaac gttaatctcc gtatgcgcca aaagctcttt tacaatactt
      601 aaatggcctt tttcacaggc aggcgtcagg ccgacaccgc caaaacgggt tacgcaatta
      661 agatcgggtt tagccggtaa aataattcgt agtagcgtta aatcatcgtt cagacagcta
      721 attaaaaaag gatttaaaca ggtatgatct tgcttattaa tatccgctcc ggcatcaatt
      781 aatgcctgaa cgcaagcata ttgctgatat aaacttgcca gcgtaattgc cgttttcccc
      841 tgacgatcgc aggtattaat atcgacaccc agcgcaaggc aggtttttac tttatcgata
      901 tcaccctgtt gtgcagccaa taaatagtcg gcaggtagat tttttatact cattcacatt
      961 cccctggttt ttgtggctgt tttcgatgaa ttgagaatag caacgataat ctgataaaag
     1021 aacccgctta aatttaattc gccgtatctc gcctttttgt tgaataatat tcaacaatct
     1081 tttcgcagtg tgcggtagtg caatatttgt ttctttgacc cgataccctt tcactcaatc
     1141 aggttttatt tctctatagt ctgcacatgt aagagtagct ttcgtccatg tctaaccaga
     1201 ggggaacaag atgaacggac tcacggcaac gggtgtcacc gtcggtattt gtgcagggct
     1261 ttggcagtta gtgtcttcac atgtcgggct ctctcaagga tgggaattat taggaaccat
     1321 tggcttcgtc gccttttgca gtttttatgc cgcaggcggc ggtaaatcgg ggtttatcag
     1381 aagtctggcg gttaattatt ccgggatggt gtgggcgttt ttcgccgcgc tgactgctgg
     1441 ttggctggcc tctgtaagtg gactgtccgc gttctgggcg agcgtaatta ctaccgtacc
     1501 cttctcggcg gtagtggtct ggcaagggcg tttctggtta ctgtccttta ttcccggtgg
     1561 ctttctcggc atgacgctgt tttttgccag cgggatgaac tggacggtga cgttactggg
     1621 ttttctggcg ggtaactgcg tgggggtaat ttcagaatat ggtggacaaa aattaagcga
     1681 agcgacgact aaacgcgacg ggtattaaaa gttattcaaa aatgattatc tatctttaac
     1741 cgatacctct acagtcatag ctggagaaat tcatagtaga taatggcgag aatggtttaa
     1801 tgaactcaat ttttaccgaa gagaatctgt tggcatttac taccgctgcg cgttttggca
     1861 gcttcagtaa agccgcagaa gagttgggtt taaccacttc cgccattagc tacaccatta
     1921 agcgtatgga gacggggctg gatgtggtgc tgttcactcg cagtacccgc agcattgagt
     1981 taacggagtc cgggcgttat tttttccgca aagctaccga cctgctgaat gatttttatg
     2041 ccatcaaacg cagtatcgat actatttcgc agggcataga agcgcgtgtg cgcatttgta
     2101 ttaatcagct gctttatacg cccaaacaca ccgcacgctt gttacaggtg ctgaaaaagc
     2161 agtttcctac ctgccagatt accgtcacca ccgaagtgta taacggcgtc tgggatgcga
     2221 ttattaataa tcaggccaat atcgccatcg gcgcacccga tacactactg gacggcggcg
     2281 gtattgatta cactgaaatt ggcgcgattc gctgggcttt tgctatcgcc ccggatcacc
     2341 cgttagcctt tgtgccggaa cccatcgccg aaagccaact gcgtctctac cctaatatta
     2401 tggtggagga caccgcgcat acgattaata aaaaagtggg ctggttgctg cacgggcagg
     2461 agtcaattct ggtgccagat ttcaacacca aatgtcagtg ccagatttta ggtgaaggaa
     2521 ttggtttttt gccggattac atggtccgtg aggcgatgac gcaatccctg ctggttaccc
     2581 gacaaatcca taatccgcgc caggattcgc gcatgttact ggcgactcag cattctgcta
     2641 cgggtcaggt gacgcagtgg attaaaaaac aatttgcgcc aaatggcata ttaacgggga
     2701 tctatcagga tttactgcat cgggagaatt aatcgctgcc aacctcccga tgcgctgtga
     2761 atagcggttt acatcaacca cctgctttca ttacccattc agagataaat ttattacccg
     2821 gtactggcgg agagtacaaa tagccctgca aaaagtgaac gcctttaccg atcattaaat
     2881 ccgcctgctc ctgggtttct accccttccg ccacgatact cagaccaggc ttacgcgcta
     2941 gttcgacaat attgtccaca atatgaccgg agatttcgtc gacactcgcc atttgcacaa
     3001 atgacttatc gatcttaata aaatcgaccg ggaacgcctg caagtaacga taggtcgcat
     3061 aacccgtacc aaagtcatcc agcgcaaagg taatgttgtg ctgatgaagg ctgtcaaata
     3121 tcgctctggc ttctggcgtt accggaatag ggttacgttc cgttagctcg agaaccagct
     3181 tgattttatc gttacctaat ttcttaacca ggttcagaca ctctttttca aatcccgctg
     3241 ccaaaaaaca acccgccgag acgttgatgc caatatggaa attgtccggc agcaaatgtt
     3301 ttaccggcat cagaatatcc gcagtctgtt tcatcagttg gcgggtcatt atgacaataa
     3361 gaccggatga ctccgccaga ggaataaact gatccggtgg gataattccc gtttgtggat
     3421 gttcccagcg gacaagcacc tcacagcccg tcagtacccc agtctgcgcg cagaaaaccg
     3481 gttggatcca cggtttgaat tcgtggtttt ccaacgccag actgatggct tcaggcgtga
     3541 cgatatggtg atagtgatta tcattaatat atctgtgact attactcccc gtggcggata
     3601 tgatctccgg attgtactga aagaaaatat cgcgccagaa agtaatatcg ctctgaatcc
     3661 ccagtttctc aaagagctgt ttcttttgat gtgaaattgt ctttgcactg cgatttctgt
     3721 actgtgatat ttctgttaca gacattccct gcatcagcag cttgagtagc agtcgttctt
     3781 tctcggatac gctaccaggc aatgacaaat gaaccgttgt accgaactct tgcagaaaaa
     3841 cacgaaaatc acatgaattc atgaacacac ctttatcttt tatcgctgtt cagtatgagt
     3901 gattacctgt cgattcctga gcacacataa ggggtaacca taaacatcag cgatgccata
     3961 cgtataacat cccacatcac tatcaagaag attatcttta cctctgatgc tcgctcaaaa
     4021 acagttacgc taaatacctt aaaaaggtaa tcacaagtca aactcaggag ataatcacgc
     4081 aagaatgcaa tattaaaatt cagatactca tttttggtaa taaagaagta gttggactat
     4141 ttcaatattt ataatttccc actacatact ggcatccatt catgagcaaa ggcgcattat
     4201 atgaatttaa caatccagat caactgaaaa tacctctccc tcataaacac atagcgtcaa
     4261 cattcaatga cataatgagt a
//
LOCUS       DACAPB010000277         4177 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.281, whole genome shotgun sequence.
ACCESSION   DACAPB010000277 DACAPB010000000
VERSION     DACAPB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.281"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..897)
                     /gene="asnS"
                     /locus_tag="IT904_004486"
     CDS             complement(<1..897)
                     /gene="asnS"
                     /locus_tag="IT904_004486"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP3194163.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSR"
     gene            complement(1066..2268)
                     /gene="pncB"
                     /locus_tag="IT904_004487"
     CDS             complement(1066..2268)
                     /gene="pncB"
                     /locus_tag="IT904_004487"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP3194164.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            2534..>4177
                     /gene="pepN"
                     /locus_tag="IT904_004488"
     CDS             2534..>4177
                     /gene="pepN"
                     /locus_tag="IT904_004488"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAP3194165.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSD"
BASE COUNT         1075 a         1072 c         1066 g          964 t
ORIGIN      
        1 acgtgaaacg gcatctttat ctacgcgttc agcgaagaat ttcatgtcgt ctgcgcgttc
       61 ttcgagaacc gctttgaaga catatttcag catggcttca gccagacccg cgatatcgtt
      121 caggttagca aacgccactt ccggctccag catccagaac tccgccaggt gacggctggt
      181 gttggagttt tcagcacgga aagtcgggcc gaaggtataa attttggata atgcgcaagc
      241 gtaggtttcg ccgttcaact ggccagatac ggtcaggaaa gactctttac caaagaagtc
      301 tttgtcgaaa tccactttgc cctgatcgtt acgcggcaga ttttccagat ccagcgtaga
      361 aacgcggaac atttcgccag ccccttcggt atcagacgca gtaatcagtg gcgttgaaac
      421 ccagaagaat ccctgctcgt taaagaagcg atgcagcgcc tgcgccagcg tatggcgaac
      481 gcgcgcgacg gcaccaatca ggtttgtgcg cggacgcagg tgagcgactt cacgcagata
      541 ctcaatgctg tggcgttttg ccgccatcgg gtaagtgtct ggatcttcaa cccaaccagc
      601 aacttcaacc ttgctggcct gaatttcaaa ttgttgcccc tggcccggcg acgccacgac
      661 tttacccgtc acaatgaccg agcaaccggt ggtcagacgc aggacgtctt cattgtaatt
      721 gggcagagaa ttattgatga cagcctgtac aggatcaaag caggaaccgt cataaacggc
      781 gaggaaggag atgccagctt ttgaatctcg gcgggtacgt acccatccgc gtacggtgac
      841 ttcgctgtca acggctacgc ggccctggag tacgtcggct acaggcacaa cgctcataat
      901 attctctctg ttaatagtcg gaaaaaataa acacttgtcc acccgtaaat ggggggatac
      961 ctatgttacc tggcatctgc aatcagacaa gcagaattcg caaatgcagc gaaagatttc
     1021 ggaattaaag gtaaaaaaag ggagccgatt agctccctga tgatattaac tggctttttt
     1081 aatatgcgga aggtcgaacg ctttgcgcag cgcccgaaca aacgctttat cgtggcagat
     1141 agttttgcca gggctgtcag aaagtttcgc caccggttta ccgttacact ctaccaactt
     1201 aatcacaata ttcaggggtt ttacctgggg gatatcgcag gtcaggcgag tcccaatacc
     1261 aaaacttaat tgcacgcggg aagagaagtg gcggtatagc tcaaccgctt tgcgtaaatc
     1321 cagattgtca gagaaaacca gcgttttact ctgtggatca attcccagct tttcataatg
     1381 tgcaatggct ttttcacccc attcaaccgg gtcgccagag tcatgacgca ggccttgata
     1441 ccgactagcg aactcgacac cgaaatcacg caggaaagca tccatagtga tgcagtcggt
     1501 taatgcaatg ccaagttggt cgggatactc ttccagccag gcagcaagtg cagctcgctg
     1561 gctgttggct agatccgggc tgatttgctg atgtgcctgg aaccattcgt gtgcctgtgt
     1621 tcccatcggc gtgagggaaa gccgacgcgc cagatcgtag ttgctggtgc ccacaaacca
     1681 ggattcctgt tgcagacgct taacgatggt ttcttgtact tcgcgagaaa aacggcgacg
     1741 ggtgccaaaa tccatcagat ggaagcgcga catatcaaga ccggcggtta acgccgagaa
     1801 gtcgactaat ttgctttcca gcgtgtcgag ggcttgcgca acgtcggcct gcggtgagcg
     1861 atagcgatgt accatttcac tgataaccgc cagcaaagga acttcccaga ggatgacttc
     1921 acgccacggg ccgcttaaac gaatatccag cttgccatta tcgttggaca cggtgacttg
     1981 ttccgggtta aagcggaact cgcgtaacca gttaagatag tcggccttaa agaaaggcag
     2041 ggcagaaagc cactgatatt catcatcctg caggcgcagg tgctgcatcg cctgaatctg
     2101 ttcacgaata gcatcggcat aaatacccag cagatcgtca cctcggcaac gaaactccgc
     2161 cgcgacatgc acatcgtaat aatgatgaaa cacggcttgc tgcatatgca acttataagc
     2221 atctgtatcc agcaacgagt gcagaacagg agaagcgaat tgtgtcatag gtgcgcagta
     2281 gcgtcctcgt acaggagcgt ttagtacaat aaacatcttc aggaactgct ggagtatacc
     2341 ttgtttcgca atttattgaa ccccgatcac accatatgcc acctttctgg tcgatggcat
     2401 tacgcggtgt atgttataaa aatgtagcaa taaaggcgtt tgtacctgaa aagatgaaga
     2461 ttctgcatag cgcgatttac gcaacaggaa tagactgaac accagactct ataaaagatg
     2521 ctaaaggtta tttatgactc aacagccaca agccaaatac cgtcacgatt atcgtgcgcc
     2581 ggattaccag attactgata ttgacttgac ctttgacctc gacgcgcaaa agacggtcgt
     2641 taccgcggtc agccaggctg tccgtcatgg tgcatcagat gctccccttc gtctcaacgg
     2701 cgaagacctc aaactggttt ctgttcatat taatgatgag ccgtggaccg cctggaaaga
     2761 agaagagggc gcactggtca tcagtaattt gccggagcgt tttacgctta agatcattaa
     2821 tgaaataagc ccggcggcga atacggcgct ggaagggctt tatcagtcag gcgatgcgct
     2881 ttgcacccag tgtgaagccg aaggtttccg ccatattacg tattatctcg accgcccgga
     2941 cgtgctggcg cgttttacca ccaaaattat tgccgataaa attaaatatc ccttcctgct
     3001 ttccaacggt aaccgcgttg cgcaaggcga actggaaaac ggacgccatt gggtacagtg
     3061 gcaggacccg ttcccgaaac cgtgctacct gtttgcgctg gtggcaggcg actttgatgt
     3121 actgcgcgat acctttacca cgcgttctgg tcgcgaagta gcactggagc tgtacgtcga
     3181 tcgcggcaac cttgatcgcg cgccgtgggc gatgacctcg ctgaaaaact ccatgaaatg
     3241 ggatgaagaa cgctttggcc tggagtatga cctcgacatc tatatgatcg tcgcggtgga
     3301 tttcttcaat atgggcgcaa tggagaataa gggtctgaat atctttaact ccaaatatgt
     3361 gctggcccgc accgacaccg ccaccgacaa agattacctc gatattgaac gcgttatcgg
     3421 ccatgaatat ttccataact ggaccggtaa ccgagtgacc tgccgcgact ggttccagct
     3481 cagcctgaaa gaaggtttaa ccgtcttccg cgatcaggag ttcagctctg accttggttc
     3541 ccgcgcagtt aaccgcatca ataatgtacg caccatgcgc ggattgcagt ttgcagaaga
     3601 cgccagcccg atggcgcacc cgatccgccc ggatatggtc attgagatga acaacttcta
     3661 cacccttacc gtttacgaga agggcgcgga agtgattcgc atgatccaca ccctgcttgg
     3721 cgaagaaaac ttccagaaag ggatgcagct ttatttcgag cgtcatgatg gtagtgcagc
     3781 gacctgtgac gactttgtgc aggcgatgga agatgcgtcg aatgtcgatc tctcccattt
     3841 ccgccgttgg tacagccagt ccggtacacc gattgtgacc gtcaaagacg actacaatcc
     3901 agaaactgag cagtacaccc tgaccatcag ccagcgcacg ccagctacgc cggatcaggc
     3961 agaaaaacag ccgctgcata ttccgtttgc catcgaactg tatgataacg aaggcaaagt
     4021 gatcccgttg cagaaaggcg gtcatccggt gaattccgtg ctgaacgtca ctcaggcgga
     4081 acagaccttt gtttttgata atgtctactt ccagccggtg cctgcgctgc tgtgcgaatt
     4141 ctctgcgcca gtgaaactgg aatataagtg gagcgat
//
LOCUS       DACAPB010000278         4101 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.282, whole genome shotgun sequence.
ACCESSION   DACAPB010000278 DACAPB010000000
VERSION     DACAPB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.282"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..224)
                     /locus_tag="IT904_004489"
     CDS             complement(<1..224)
                     /locus_tag="IT904_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transpeptidase"
                     /protein_id="HAP3194166.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKIC"
     gene            complement(380..658)
                     /gene="yafQ"
                     /locus_tag="IT904_004490"
     CDS             complement(380..658)
                     /gene="yafQ"
                     /locus_tag="IT904_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HAP3194167.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(661..921)
                     /gene="dinJ"
                     /locus_tag="IT904_004491"
     CDS             complement(661..921)
                     /gene="dinJ"
                     /locus_tag="IT904_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HAP3194168.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1107..1880
                     /locus_tag="IT904_004492"
     CDS             1107..1880
                     /locus_tag="IT904_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP3194169.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALVENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            2056..2553
                     /gene="rayT"
                     /locus_tag="IT904_004493"
     CDS             2056..2553
                     /gene="rayT"
                     /locus_tag="IT904_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAP3194170.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVERGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(2769..>4101)
                     /locus_tag="IT904_004494"
     CDS             complement(2769..>4101)
                     /locus_tag="IT904_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAP3194171.1"
                     /translation="LSTAAAIIVTRVSDNGDIAHDVRHQLLASPSVLYTATGIMFVLA
                     MVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSLETLTRTITETSEQQVSWETI
                     PLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVISDGNGVLLPEIRIRENFRLKP
                     SQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVLGNDPAYGMPVTWIQPAQKAK
                     ALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQLHNRLASMAPRLAEDLSAAL
                     NYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKDHILLAADVRLALRRSITHPF
                     VRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDSVPVDPNMLNQFQSTMPQVKEQM
                     KAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEVPDELELKIMGALM"
BASE COUNT          975 a         1069 c         1021 g         1036 t
ORIGIN      
        1 ttacagattt tatagctgtc gagcagttga tattgctcgc ccattttgac gtagagatcg
       61 agcgtacgtt cttccttgaa gatctggatg tagacagggg atcccatcaa ctgctgctta
      121 tactctttgc tcactggcgt ggtcgagcta ctgctgccca gcaaaccggc aaacgaaacg
      181 cacgggatca acaacatcgc aagaattaat gcgattttac gcatacagct tattccttga
      241 taaaacggtt acacacgcca ggacggcaaa atggatccca aatcggaata gtctggattt
      301 ggaaggctca cattatcacc aaaagagttt tacgcaagcc tgtcggcgca gggtttacaa
      361 atttcatcgg aagcgggctt tacccaaata gcgccgcgtg agttccagtt ctctcaaatc
      421 gtaaaagttt atcggtaagt ttgtaaatca ggatccagtc cggttcgaca tgagcatcgc
      481 gataaccttt ccatgaacct tgcagcgggt ggtctttata aacagctgga agcggtaaag
      541 tattattgat aagaagcgtc ataagatatt tcaatttatt catatcctta tgacgctttt
      601 gtgcaagttt tacatccttt gaaaattgtc ccgagtattc aatatccctt tgaatcatat
      661 ttaaattcct aatttatcaa ataaatcatc ggcgtctttg gccttatgaa catcaacgcc
      721 agcttcgctg tttttaattg attgaatggt taattgatta ggctcgcgta aatcaaacgg
      781 caatgccttt tcacgcgcga cttttgtgag ggttatgcga accaggtcag agatggtcag
      841 ccccatcccg gccagtacgt ccgccgcctg attcttcaga tcttcatcga ttcgggcgcg
      901 aacaaacgcg ttagcagcca ttggggtatc tcctgtgctg tatatttatt cagcttgaat
      961 tgtagctcaa atgagcaaca gcgacaatcc atgagtaatt taataaacgg atgtttttac
     1021 ataattaact gaaatatatt tattaatttt ataataatgg tttattaagt ttgttctggt
     1081 acgggccggg atacactgca acatcaatgc atgaccagcc attattgaga atgtccttgc
     1141 cgtcgattcc ctcttttgtg ttgtcgggat tactgttgct ttgcttgccg ttttcttcat
     1201 ttgccagcgc caccacatca catatctctt tcagctacgc cgcccgccag cggatgcaaa
     1261 accgtgcgcg tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctatattg
     1321 tggaaggcaa tgccgaaagc agaagggcgc tacgccagca caaccgcgaa cagataaaac
     1381 agcatccaga atggtttcct gcaccgctca aggcgagtga cagacgctgg caggcgctgg
     1441 tggagaataa tcatttctta agcagcgacc atctgcataa catcaccgaa gtggcgattc
     1501 accgcctgga acagcaactt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag
     1561 gcttcgactg tagcgggtta gttttttatg cctacaacaa gatcctcgag gcgaagctcc
     1621 cacgtacggc caatgagatg tatcactatc gccgggcaac gattgtggcg aacaacgacc
     1681 tgcgccgggg agatttgctg tttttccata tccacagccg cgagattgcc gaccatatgg
     1741 gggtgtatct gggcgacggt cagtttattg agtcaccgcg caccggggaa accattcggg
     1801 taagtcggtt agctgaacct ttctggcagg atcatttttt gggcgcgcgg cggatattga
     1861 cggaagagac gattttgtag gacggataag gcgtttacgc cgcatccggc ggtggtgcgc
     1921 agatgcctga tgcgacgctg acgcgtctta tcatgcctac aaatctgtgc gtttttgcga
     1981 gccgctttcc tgttattcca tcacctcatt gccgaccttt ccttttcttc ttaccgttga
     2041 gaaaaaggag tcgccatgtc tgaatatcgt cgttattaca tcaagggggg aacctggttt
     2101 ttcacggtga atttacgcaa tcgtcgaagc caactcttga ccacccagta ccagatgctc
     2161 cgtaacgcca ttattaaagt taagcgagac aggccttttg aaatcaacgc ctgggtcgtt
     2221 ttgccagagc atatgcattg catatggaca ttacctgaag gcgatgatga tttttcctct
     2281 cgctggcggg aaattaaaaa gcaatttacc catgcttgtg gattgaaaaa tatctggcaa
     2341 ccacgttttt gggagcacac catccgcaat accaaagatt ataggcatca tgttgattat
     2401 atttatataa atccagtaaa acatggttgg gtaaagcaag tgagtgattg gccattctca
     2461 acgttccatc gcgatgtcga gcgagggtta tatcccatcg attgggcggg ggacgtaacg
     2521 gatataaatg ctggcgagcg tatcatttta taattatgcg cagatgcctg atgcgacgct
     2581 ggcgcgtctt atcgtgccta caaacttgtg ccgcatccgg caattgtgcg cggatgcctg
     2641 atgcgacgct ggcacatctt atcatgccta caaacctgcg cctgaacggt aggccggata
     2701 aggcgttcac gccgcatccg gcggttatgc accgatgcct gatgcgacgc tgacgcgtct
     2761 tatcatgcct acatcaacgc ccccataatc ttcaactcca gctcatccgg cacttcgtta
     2821 tacgacagca catgcagccc cggcgcaaac aaccttgcat aacgcgccag caaagggcgc
     2881 agctgcggtg gcaccagcag caccgggtct ttccccgccg ctttcatctg ctctttcacc
     2941 tgtggcatcg tgctctggaa ctggttgagc atattcgggt ccactggcac gctgtcaagc
     3001 atcactttcc cgccctgttg cgcctgattc accacattgg tcagcagatt ttccagttca
     3061 ttattcagcg tatacaccgt cagctcctgc ttgcgaacga acggatgggt aatgctgcgc
     3121 cgcagcgcca ggcgcacatc agctgccagc agaatatgat ctttggtcac cgcgctactg
     3181 gcgaccagca cggtggcgat ggtgacgata tcgcgcaggg aaacgccttc ggtcagcagc
     3241 gcccggtaca ctttcagcaa ctggctgtaa ttgagcgccg cgcttaaatc ttccgccagg
     3301 cgcggtgcca tcgacgccaa acggttatgc aactgcgtaa tatcatcata gttaaacaaa
     3361 tcaggaatat agctgcgcac aatcttgttc acatgggtag caatcacgct ggcgctgtcg
     3421 atcacctgat accccatatt cagcgccttc gccttctgcg caggctggat ccaggtgacc
     3481 ggcatcccat acgccgggtc gttccccagc acgccgtcaa tctcgccgta ggtttcgctg
     3541 gagggcaggg ccatcagttt atccgccgga atatccgctt catcagcctt aatgccgttg
     3601 atgaaaatag cgtactggct gggcttaagg cggaagtttt cccgaatgcg gatctccggc
     3661 agcagcacgc cgttgccgtc agaaatcacc tgtcgcacgc cgcgaatacg ctgggtgagc
     3721 gggttgccct gggctttgtc caccagcgcc accagcttgt agccgaggct taagctgatg
     3781 ggctcgatca gcggaatggt ttcccaactg acctgttgct cgctggtttc ggtgatggtg
     3841 cgggtcagcg tttcgagact tttctcttcc gcttccgccg cctgcgggcg tttgctcatc
     3901 cgccagccgg taaagccaag cagggcgctg aacagcaaaa acggcagatg cggcattccc
     3961 ggcaccatcg ccagcacaaa cataatcccg gtagcggtgt agagcaccga cgggcttgcc
     4021 agcagctggt ggcgcacgtc atgggcgata tcgccgttgt cgctgacgcg ggtgacgata
     4081 atcgccgccg ccgtggagag c
//
LOCUS       DACAPB010000279         4071 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.283, whole genome shotgun sequence.
ACCESSION   DACAPB010000279 DACAPB010000000
VERSION     DACAPB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.283"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            300..1922
                     /locus_tag="IT904_004495"
     CDS             300..1922
                     /locus_tag="IT904_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194172.1"
                     /translation="MQQKYQYDRVLSGELHPFPGIYEAVSEQILLLIFKARQLYQDPE
                     YRPWFEEQHRVLSALRANTELLRIFREQRSTEEPKLTIEESTSPQPTPSALTDALNVM
                     CHLYNGFDEGNISLKTWGAMQEVQTTSTFISPGPRTWKTPELAKFVGDDLFNILTECK
                     AWSSPIPAETRQGYCHQDLSVAHSCTSELLAHLNQYHEIDESAILARVIGITVNGFYK
                     KNNTDLPEWLSPFKEEHKIFKDCPEQNTGDVIRRKEQLLKTGRTEFVNNLCRVANWYE
                     IESIYDEYVRLYANEIIDWFNKTMKYFKPTDFIRYTWKFATVTPDKDTETGHTTAWEK
                     DYCQITIQRDELLRLIHTNTLQDAVIYLCHCLQEKYKKSSQNETEFPGIKPPASESEI
                     NITDNFEFHSLLELSKDNEKRLETFQKWYEKNKVLLESHKQKRTEKIDVAVRLVGLKA
                     YDLHEGIPDSPKRKVKDGINEDIKADTSLRLSKTNISDTSLNRYRRTVKEIINNEIDA
                     FLLEQKKKNKRFPYSEDRDVIRPLWGKCLTGQ"
     gene            complement(2009..2605)
                     /locus_tag="IT904_004496"
     CDS             complement(2009..2605)
                     /locus_tag="IT904_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194173.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVSL
                     ATFVYETIATKAGVILLSDNEQYEGGKALWQHIARRSTNFKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            3297..3728
                     /locus_tag="IT904_004497"
     CDS             3297..3728
                     /locus_tag="IT904_004497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194174.1"
                     /translation="MKKSLVLVMFLFSACVTASNDLDLYDNQGNAVVYISLDDELTLY
                     SWEGEPSAYLKHNQNNEFDVYGFNGNHLGWFTNGMLIDHDGYVACAVKDMVTTPNLPS
                     LKSLKSLKPLKSLTELPPLFPLLKNAFGQTNCSLLVASGVA"
BASE COUNT         1329 a          862 c          814 g         1066 t
ORIGIN      
        1 ggaggaaaag acaatctgtt gctgaacaga cagaagcagg ctacgaatga tcggaaaatg
       61 aagcaaatac gcttatacag aagacgcaga ggtaactgac ggagacaatc acaatcagga
      121 gaaaattatc gtaaataatg tataaaatct caggatataa acaaatgtac acaagctatc
      181 cacgtaatca gcggctggct caggattgac gtttcatttc agtggacaac atcgattctg
      241 tctgacagcg gaccaatatg tagcaagcag aaacttctga catcatggta actcggacaa
      301 tgcaacaaaa gtatcagtat gatcgtgttt tatccggcga actccaccca ttccctggca
      361 tatacgaggc ggtttccgaa caaatccttt tgctcatttt taaagcacgt caactatatc
      421 aggatccaga atatcgcccc tggtttgaag aacagcacag agtactctct gcattacgtg
      481 ccaatacaga gctcctgaga atctttagag aacaaagaag cactgaagaa ccaaagctta
      541 ctattgaaga gtctacctcc ccccaaccca ctccttctgc tcttacagat gcgcttaatg
      601 tgatgtgcca tttatacaat ggttttgatg aaggaaatat cagtctgaaa acctgggggg
      661 cgatgcaaga ggtacagaca acatcaacct ttatcagccc aggaccgaga acctggaaaa
      721 cacctgaact ggcaaagttt gtaggagatg atttatttaa tattctgact gaatgtaagg
      781 cctggagttc accaattcca gcagaaaccc gacaggggta ttgccatcag gatctgtctg
      841 ttgcacactc ctgcacctca gagttactgg cacacctgaa tcaatatcat gaaattgatg
      901 aatcagcaat acttgccaga gttattggta taacagttaa cggattctac aagaaaaata
      961 atacagacct tccagaatgg ctatccccct ttaaagagga acataaaata ttcaaagatt
     1021 gtccggagca aaataccggg gatgtaatca gacggaaaga acaactgttg aaaacaggac
     1081 gtacagaatt cgtgaataat ttgtgtcggg tcgccaactg gtatgaaatt gagtcaatat
     1141 acgatgaata cgtccgtctt tatgccaatg aaatcattga ctggttcaac aaaaccatga
     1201 agtattttaa accaacagat tttatccgat atacatggaa attcgcaaca gttactccgg
     1261 ataaagatac agaaaccggg catacaactg catgggaaaa ggattattgc cagataacca
     1321 tacaacgtga tgaacttctg aggttgattc acactaatac ccttcaggat gctgtaattt
     1381 atctttgtca ttgtctgcag gagaaataca agaaatcatc ccagaatgaa acagagtttc
     1441 caggaataaa acctccggca tcagagagtg aaattaatat aacagataat tttgaatttc
     1501 attctcttct ggagctaagt aaagataatg agaagcggct ggaaacattt caaaaatggt
     1561 atgaaaaaaa taaagttttg ttagaaagtc ataaacaaaa acgaacagag aagattgacg
     1621 ttgccgtacg acttgtaggg ctgaaagcct atgatctgca tgaagggatt ccggatagtc
     1681 caaaacgaaa agttaaagat ggcataaatg aagacatcaa agccgatacc agtttacgcc
     1741 tgtcaaagac aaatataagt gatacgtcgc taaaccgtta tcgccgcact gttaaggaga
     1801 ttatcaataa cgaaatcgac gcattcctgt tggaacagaa gaaaaaaaat aaaagatttc
     1861 cctacagtga agatagagat gtgattcgcc ctttatgggg aaaatgcctg accgggcagt
     1921 agatcacaga tgggataaca tcccggttat cccctgtttg ttctgcttca ggttgccgga
     1981 gctccaggaa cacgaggcaa tcggcaaatt atgcggcttt tccattaaca gattcggcca
     2041 caagaacaac accgtgtttg tttcgatcag gatgttcact ccagatttct gattcaggaa
     2101 tactttcccc atcatagcag atccgatcac catcaaacgg ataataccgt gcggaatctg
     2161 tatctaagat aaacacctta aaattcgttg aacgacgcgc tatatgctgc cagagggctt
     2221 tacctccttc atattgctcg ttatcactta atagtataac gcctgctttt gtggctattg
     2281 tttcataaac aaacgtagca aggctaacat cacgtacagc gccatcgaca acaatccctt
     2341 caatctgagc aacgtcctgt tcttcccaga atttccggac aaacacccct tcaagctgtg
     2401 aacgacgctg aagctccaga gtggcaacaa ccttatggcg aatatcacca tcaataacag
     2461 aaggtgcagc ttcgattacc gcagcgaacg gagtctttgg atcccagatg atctggtaaa
     2521 gcacaccctg aatagttact ccatccagta tcgaaaaacg acgagctaca gtagcagggg
     2581 agtaagacat tggaatgaca gccattttat ggcctaaaat ttgaaaacat cagtatcaat
     2641 actgacctca tcagtctgag catatattga taaagttttt atgtttacac tcaataaata
     2701 ccttgccaaa caactaatag cgatgtctac attgccattc gacatgctta caatttttgt
     2761 caacaagaaa gtgaatttat atcaacggta agaagtaaac acgaggacac caaaaccaaa
     2821 tgtacacact tttgtgcttt gtgtcccctg tgtgtaccca aatgacaaac tgcaagaaaa
     2881 tctaggcaca cacttatcta acttattgat ttaaatggtg ccgataatag gagtcgaacc
     2941 tacgaccttc gcattacgaa ttataagaat ccacttctaa ttcaaagcat taccccatca
     3001 acactgcgct cacacgtccc accacatcaa aacatgtaaa gccttgcaag ccattgcgag
     3061 gccttatgtg tctcagtttt gtcccacctt gtattacgac ttgcatagcc aatgaagata
     3121 aacgtgacga caaacggcgc agcagtcttc ttttccttca tactttcccc acccagcatg
     3181 cataccttct accataactg tagtgaatgt ctgttatgag cgaaaatcgg aagttagtgt
     3241 cctcataatt ttgatagaat gttcgaactt ttcctttcta agaaacttga gaagttatga
     3301 aaaaatcact cgtgctagtt atgtttcttt tctcagcttg tgttactgcg agtaatgacc
     3361 tagatctata tgataatcaa ggcaacgccg tcgtttatat ttcacttgat gacgagttga
     3421 ctttatatag ttgggagggt gaaccaagcg cctaccttaa acataaccag aacaatgagt
     3481 ttgatgttta tggattcaat gggaatcatt taggctggtt tactaatggt atgttgattg
     3541 atcacgatgg ctatgttgcc tgtgcagtaa aagacatggt taccacacct aatttgccat
     3601 cacttaaatc acttaagagc ctcaagcctc taaaatcgct aacggaactt ccccctcttt
     3661 tcccgctgct aaaaaatgcc tttggtcaga caaattgttc tttgttagta gcttcaggtg
     3721 ttgcataaat ccacaacgga tgcatttctt gaaagggatg cccctccaga ggataaaatg
     3781 cttacggtat ccgctcctga cacggagcgg accaatcagg tggttgtaag gtccgctatg
     3841 agcaagaagc ggaagttggc agttttgtgg actgtcccca caaaagtgac tacagaaata
     3901 gttgcaattc ataattgatc atgggttgtc agttaaactc gtggcgattt aaatagacta
     3961 attgggagtg cgtccattac ttatatcttg taatgttaac tatcagaaat gatacaaaga
     4021 taatatgtct ttaaagaaaa gactgatggc gaaaagtggc ccgatgaggg c
//
LOCUS       DACAPB010000280         4020 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.285, whole genome shotgun sequence.
ACCESSION   DACAPB010000280 DACAPB010000000
VERSION     DACAPB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.285"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            82..1218
                     /gene="potA"
                     /locus_tag="IT904_004498"
     CDS             82..1218
                     /gene="potA"
                     /locus_tag="IT904_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAP3194175.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1202..2065
                     /gene="potB"
                     /locus_tag="IT904_004499"
     CDS             1202..2065
                     /gene="potB"
                     /locus_tag="IT904_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAP3194176.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(2298..2563)
                     /locus_tag="IT904_004500"
                     /pseudo
     CDS             complement(2298..2563)
                     /locus_tag="IT904_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2620..3288
                     /locus_tag="IT904_004501"
     CDS             2620..3288
                     /locus_tag="IT904_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP3194177.1"
                     /translation="MVKLMKKNTDHGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(3343..3477)
                     /locus_tag="IT904_004502"
                     /pseudo
     CDS             complement(3343..3477)
                     /locus_tag="IT904_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(3526..>4020)
                     /locus_tag="IT904_004503"
     CDS             complement(3526..>4020)
                     /locus_tag="IT904_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HAP3194178.1"
                     /translation="TDGQNLTVNGSVNCTTLNQSSDRRLKENIEIIDNATDAIRKING
                     YTYTLKENGAHCAGVIAQEVEEAIPEAVGSFIHYGEELQGPTVDGNELREETRYLNVD
                     YAAVTGLLVQVARETDDRVTALEEENTTLRQNLATAGTRISTLENQVSELVALVRQLT
                     GSEH"
BASE COUNT          988 a          984 c          945 g         1103 t
ORIGIN      
        1 cgtagaatta ccgccctcat taagagtcac caaggtggtt aaccacaaac cccgcatcgg
       61 taagccatcc gttgcgttta catgggacag agtaaaaaat tgaataaaca accgagttcg
      121 ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa agaggtcatt
      181 ccccagctgg atctgactat caacaatggc gagttcctca cgctgcttgg cccttctggc
      241 tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg aaactgttga ttccggacgc
      301 atcatgctgg ataacgagga catcacccac gttccggcgg aaaaccgcta tgtgaacact
      361 gttttccaaa gctacgcact tttcccccac atgaccgtgt tcgaaaatgt ggcctttggg
      421 ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga agccctgcgg
      481 atggtgcagc tggaaacctt cgctcaacgc aaaccgcatc agctctctgg tggtcaacag
      541 caacgcgtcg ccattgcccg cgcggtggtt aacaagcctc gtctgttgtt gctggatgag
      601 tcgctctcag cgctggatta caaactgcgc aagcaaatgc agaacgagct gaaagcgtta
      661 cagcgtaagc ttggtattac cttcgtcttt gtcactcacg atcaggaaga agcgctcacc
      721 atgtcagaca ggattgtggt gatgcgcgag ggtcgtattg agcaagatgg tacaccgcgc
      781 gaaatttacg aagagccgaa aaacctgttt gttgccggct tcattggcga aatcaatatg
      841 tttaacgcca ctgtcatcga acgtctcgac gagcagcgcg tacgcgccaa cgttgaaggc
      901 cgcgaatgta atatctacgt taacttcgcc gttgaaccgg ggcaaaaact gcatgttctg
      961 ctgcgcccgg aagacttacg tgttgaagag attaacgacg acaaccacgc tgaagggctg
     1021 attggttacg ttcgtgagcg taactacaaa ggcatgacgc tggagtcggt tgttgaactg
     1081 gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg aagacgaccc tgactttgac
     1141 cactctctcg accaaaaaat ggccattaat tgggtagaaa gctgggaggt cgtactggct
     1201 gatgaagaac acaagtaagt tccagaatgt agtgattgtc actattgtcg gttggcttgt
     1261 gttgtttgtc tttctgccca acctgatgat cattggcacc agctttttga cccgcgacga
     1321 cgccagtttc gtcaaaatgg tctttacgct ggataactac acgcgtctgc tcgatccgct
     1381 ctattttgaa gtgctattgc actcgctgaa tatggcgctg atcgccaccc tcgcctgcct
     1441 ggtgctgggc tacccgtttg cctggtttct ggcgaagctg ccacacaagg tgcgtccgct
     1501 gctgctgttt ctgctgattg ttcccttctg gaccaactca ttgattcgta tctacgggct
     1561 gaaaattttc ctcagcacca aaggctatct caacgagttt ttgctctggc tgggcgttat
     1621 cgacacacca atccgtatca tgttcacgcc cagtgcggtg attatcggtc tggtttacat
     1681 tctgctgccg tttatggtga tgccgctgta ctccagtatc gaaaaactgg ataagccgtt
     1741 gcttgaggcg gcgcgcgatc tcggtgccag caagttacag acttttatcc gcataattat
     1801 tccactgacg atgccgggga ttattgccgg atgtctgctg gtgatgctgc cagcgatggg
     1861 cctgttctat gtgtccgacc tgatgggcgg tgcgaaaaac ctgctgatcg gtaacgtcat
     1921 caaggtacag ttcctcaata ttcgtgactg gccgtttggt gcagccacca gcattacgct
     1981 gactatcgta atgggcctga tgttgctggt ttactggcgc gcttctcgtc tgctgaataa
     2041 gaaggtgagc gaattagatg attaatcctt tatattcaat gcattaggtt tgtttttgag
     2101 tggttttgcc gtttaaggta acggcattat agatcctatc aaaatcaatc aattgcattc
     2161 tggttttgga gaaagatttt tcgagaactt cggtctttaa ctaaaactga ttgcgtataa
     2221 tcccaatccc ccctccccga ctatctgaat ggtcggggag gcacttcaag cacaaaatat
     2281 catccctgcg ccgttgatta ctctgcaggt gaagtcggcc actcaatatc aggtgcagtt
     2341 gatgtatcaa cacgattcaa caacacccga tatttattcc atgcctccag caacgatctt
     2401 tcttcctccg ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcactg
     2461 aattcctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
     2521 gaagcaacta ggctatttca atatcgctat gctgcggcag catttaaccc cttgtaattc
     2581 atcgccataa ttgatttaat tcacaaataa aactataaca tggtgaaatt aatgaaaaaa
     2641 aacacagatc atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg
     2701 gttttgggag tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca
     2761 cactttctgg aacatttagg taacaaccat ctggatattg gtgttggaac tgggttttac
     2821 cttactcacg tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg
     2881 aacgcggcat ctacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt
     2941 tttgaacctt atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt
     3001 cttcactgcc tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag
     3061 gccttaactg acgatggaac tctatacgga gccacaattc ttggcgatgg agttgtgcac
     3121 aatagcttcg gtcaaaaact gatgcgcatt tacaatcaga aaggtatctt ttcaaacaca
     3181 aaagattccg aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc
     3241 aaggttcaag gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccaaccgca
     3301 gcacgttctt gcttaagacg tgctgcggca taatcccaat gattactccc tgacagggtt
     3361 cgtaggccac tcaatatcag gtgcagttga tgtatcaaca cggttcagca acacccgata
     3421 ctttttccag acttccagca acaaggtttc ttcctccgtt gcgatttcca gctcaacaac
     3481 agtctgaacg taccgggaac agcctccttc agagcttgaa ggatattaat gttcgcttcc
     3541 tgttaactgc cggacaagtg caaccagttc gcttacctga ttttccagag tgctgatccg
     3601 ggtgcctgct gttgccagat tttgacgtag cgttgtgttt tcctcttcca gcgcggtaac
     3661 gcgatcatct gtttcacggg cgacctgaac aagtaaaccc gtcacggcgg cgtagtcaac
     3721 attaagatag cgcgtttctt cgcgtagctc gttgccgtca acggtcgggc cttgcaactc
     3781 ttcaccgtaa tggatgaatg aaccaacggc ctccgggatg gcctcttcaa cttcctgggc
     3841 tataaccccg gcgcaatgtg ccccattctc cttgagcgtg tacgtgtatc cattaatttt
     3901 gcggattgcg tcagtcgcgt tatcgataat ctcgatattt tctttcagtc tgcggtctga
     3961 tgactgattc agtgtggtgc agttaacact tccgtttaca gtaaggtttt gcccgtctgt
//
LOCUS       DACAPB010000281         3973 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.286, whole genome shotgun sequence.
ACCESSION   DACAPB010000281 DACAPB010000000
VERSION     DACAPB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.286"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..442)
                     /gene="slyB"
                     /locus_tag="IT904_004504"
     CDS             complement(<1..442)
                     /gene="slyB"
                     /locus_tag="IT904_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAP3194179.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNG"
     gene            716..1825
                     /gene="anmK"
                     /locus_tag="IT904_004505"
     CDS             716..1825
                     /gene="anmK"
                     /locus_tag="IT904_004505"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAP3194180.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLTTIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1923..2252
                     /gene="mliC"
                     /locus_tag="IT904_004506"
     CDS             1923..2252
                     /gene="mliC"
                     /locus_tag="IT904_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAP3194181.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2311..2967
                     /gene="pdxH"
                     /locus_tag="IT904_004507"
     CDS             2311..2967
                     /gene="pdxH"
                     /locus_tag="IT904_004507"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAP3194182.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3096..>3973
                     /gene="tyrS"
                     /locus_tag="IT904_004508"
     CDS             3096..>3973
                     /gene="tyrS"
                     /locus_tag="IT904_004508"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP3194183.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKN"
BASE COUNT          989 a          988 c         1019 g          977 t
ORIGIN      
        1 tgccattgct ggccagtacg acacgttggc ccggagagaa acgagtgttg ccttgtttct
       61 gtaccaccat gatggtatta ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt
      121 tgttcattgc actctgtacg ccctgaccag ctacgccacc tgcaacagcg cctgctgcag
      181 tagccagaga acgcccggtt ccgccaccaa cagtattccc caggaaacca ccaagaacag
      241 caccgccaat tgcaccgata acgttggaat catcaccgcc ctgaatctgt accggacgta
      301 cgttaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa gcggtataaa
      361 catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct accattgaaa
      421 caaccaatac gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc
      481 atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt ctaccaaata
      541 tgagcaaatc ataatgaaag taggatcaac caattataaa caaaacattc atgatgatta
      601 ttgatttgaa gataatgttt tactaccaac ggtaaaaatc ggtcattccg catggtattc
      661 acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa
      721 atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg atgttgtgtt
      781 gacgacaatt gatgaacacc gggtcgcaca gctggcaagt ttgagttggc cgatcccggt
      841 atctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac tttcgcagtt
      901 tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa
      961 ggaacaaaat ctgcaggcaa gagatatagt tgcgatcggt tgtcacggtc aaaccgtctg
     1021 gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca atcaaattgt
     1081 ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg
     1141 acaaggcgca ccgctggtac ctgcgttcca tcatgcgctg ctggctcacc caaccgagcg
     1201 acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc ctgggcagcc
     1261 ggttgggggc tacgataccg gtcctggtaa catgctgatg gatgcctgga tctggcgtca
     1321 ggccggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag ttattctccc
     1381 actgctgcaa aatatgctca gcgacccgta tttctcgcaa cctgcaccga aaagcaccgg
     1441 acgcgaatac tttaactatg gttggctgga gcgccatttg cgccattttc cgggtgttga
     1501 tccccgagat gtgcaggcga cactggcaga actcaccgcc gtgaccattt ctgaacaagt
     1561 tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc gtaatccgct
     1621 actcatggcg cgtctggcgg cattactgcc aggcacagaa gtcaccacca ccgatgccgt
     1681 tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct ggcggacgct
     1741 ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga cggtactggg
     1801 ggctattttc cccgctaacc cgtgatatca gagttaactg aatttttctt accgcgtctg
     1861 ccgttacact tggaacgtta gggagggcgt attgccctcc agaccaggaa agtcttcggg
     1921 atatgactat gaaaaaactg ttaattatca ttttgccggt ccttctctcc ggctgtagcg
     1981 cctttaatca gctggttgag cgcatgcaaa ccgatacgct ggaataccag tgtgatgaaa
     2041 aaccgttgac ggtcaaactg aataatccgc gccaggaggt cagttttgtt tacgataatc
     2101 aactactgca tctcaaacag ggcatttcag cctctggcgc gcgttacact gacggaatct
     2161 atgttttctg gtcgaaaggc gatgaagcga ctgtctataa acgcgaccgc atcgtcttga
     2221 ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc gcacaatagc
     2281 gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt gcagcaaatc
     2341 gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct tcccgccgat
     2401 ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact ggcggaccct
     2461 accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg catcgtttta
     2521 ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggcag ccgtaaagca
     2581 catcaaatcg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgagcgc
     2641 caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat gaaatatttt
     2701 catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc cagtcgcatt
     2761 tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacagggc
     2821 gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca gattgagttc
     2881 tggcagggtg gtgagcatcg cctgcatgac cgctttttgt accagcgtga aaatgatgcg
     2941 tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta atcgctggta
     3001 ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg
     3061 gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac
     3121 aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac
     3181 tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt
     3241 tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg
     3301 ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgccgagc
     3361 gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt aagcaggttg
     3421 ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact
     3481 ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta
     3541 accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt
     3601 cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac
     3661 agtacggtgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc acttctggta
     3721 tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca
     3781 ctaaagcaga tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga
     3841 agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt
     3901 accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc ctggaagaag
     3961 aagataaaaa cag
//
LOCUS       DACAPB010000282         3909 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.287, whole genome shotgun sequence.
ACCESSION   DACAPB010000282 DACAPB010000000
VERSION     DACAPB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.287"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            2..370
                     /locus_tag="IT904_004509"
     CDS             2..370
                     /locus_tag="IT904_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194184.1"
                     /translation="MTCAQWLWKKIIALYEQAAECDGEVVRPKEPNWTAWANEIRLMC
                     VQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELSLRLSPSVSTYTEKREDP
                     YFKSSYDNVDYSQIPAGFRG"
     gene            367..861
                     /locus_tag="IT904_004510"
     CDS             367..861
                     /locus_tag="IT904_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAP3194185.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSSDEWYLSGN
                     YVGA"
     gene            861..1514
                     /locus_tag="IT904_004511"
     CDS             861..1514
                     /locus_tag="IT904_004511"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAP3194186.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLEQVRQQAEKLVREMAA"
     gene            1511..1837
                     /locus_tag="IT904_004512"
     CDS             1511..1837
                     /locus_tag="IT904_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP3194187.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRVLEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1834..2223
                     /locus_tag="IT904_004513"
     CDS             1834..2223
                     /locus_tag="IT904_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP3194188.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPAAVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            2243..3040
                     /locus_tag="IT904_004514"
     CDS             2243..3040
                     /locus_tag="IT904_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAP3194189.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3048..>3909
                     /locus_tag="IT904_004515"
     CDS             3048..>3909
                     /locus_tag="IT904_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAP3194190.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVL"
BASE COUNT          920 a          963 c         1158 g          868 t
ORIGIN      
        1 tttgacctgc gcacagtggc tctggaaaaa aatcatcgcc ctgtacgagc aagccgccga
       61 atgtgacggc gaggtggttc gtcccaaaga accgaactgg acagcctggg caaacgaaat
      121 tcgcctgatg tgtgtgcagg atggtcgtac tcacaaacaa atctgcgaga tgtacagccg
      181 cgtcagccgc gatccgttct ggtgccgtaa cgtgctcagc ccgtcgaagt tgcgggaaaa
      241 atgggatgag ctttccctgc gcttatcacc gtccgtcagc acgtacaccg aaaaacgcga
      301 agacccgtac ttcaaatcca gttacgacaa cgtggactac agccagatcc cggcaggatt
      361 cagggggtga tcatgagtct gttaaatgac gttcagaaat tcattgaagc ccatccgggg
      421 tgtacttccg gagacattgc ggatgctttt gcaggttact cacggcagcg cgttctgcag
      481 tctgcaagca agttacgtca gagtgggcgt gtggctcacc gttgtgaagg agatacacgc
      541 agacatttcc cgcgcctgac tgagagagcg caggagccgg aaccacaacc agttcgtgaa
      601 accagacctg tgcgcaattt ctatgtcggc actaacgatc cccgggtgat tttgtgcctg
      661 acccgccagg cggaagaact ggagtccagg ggcttatacc gtcgtgctgc aacggtgtgg
      721 atggcggcat tccgtgaaag ccactcccag ccagaacgaa acaattttct ggcgcgtcgt
      781 gagcagtgtt tacggaaaag cagcaagcgc gctgtatcga gtgatgagtg gtatctgtca
      841 gggaattacg tgggggctta atgagtaata aatattgcca ggcgctggtg gaactacgga
      901 acaaaccagc ccatgaactg aaggaagtgg gcgatcagtg gcgcacgccg gataacattt
      961 tctggggaat taacactctg tttggcccgt ttgttctgga tctttttact gacggtgata
     1021 acgccaaatg tgccgcgtat tacacggcgg aagacaacgc gctggcgcat gactggtcag
     1081 aacgtcttgc ggagcttaaa ggtgctgcct ttggtaatcc cccatacagc cgcgccagtc
     1141 agcatgaggg gcaatacatc accggcatgc gttacatcat gaaacatgcc agtgccatgc
     1201 gtgataaggg cgggcgctat gttttcctga tcaaagctgc caccagcgaa gtgtggtggc
     1261 cggaagatgc agatcatatt gcttttattc gcgggcgtat tggttttgaa ctgcctgcct
     1321 ggtttatccc gaaggatgag aagcaggtgc cgacaggcgc tttcttcgct ggtgctattg
     1381 ctgttttcga caagacctgg aagggaccgg caatcagcta catcgggcgc gatgaacttg
     1441 aggcatgtgg tgaggcgttt ctggagcagg ttcgtcagca ggcggaaaaa ctggtcaggg
     1501 agatggcggc atgacgacgt taactcaatg ccagcagcag gtgctggata tgctgatttc
     1561 ttatcagaaa gaacgtggtt tcccgccaac caatcaggag gtggcaacca tgctgggata
     1621 ccgttcggtg aatgcagcgg tggagcatct tcgtgtgctg gagaaaaaag gcgtcatcac
     1681 gataaagcgt ggcgtggccc gggggataac gcttcatacc gcggtgaagg acgacgacag
     1741 cgaggcggtc gggattatcc gctcactgct tgccggtgag gaaaacgcca ggctgcgtgc
     1801 agcccactgg ttacatgaga ggggcctgaa agtatgaagc tgattctgcc ttttccgccc
     1861 agcgtgaaca cgtactggcg acaccccaac aaaggggcgt ttgctggtaa gagcctgata
     1921 agcgcggcgg ggcgaaaatt ccagagcgcg gcgtgcgcag caatagttga gcagttacgt
     1981 cgtctgccga aaccaacgtc ggcacctgct gcagtggaga tcgtgttgtt tccaccggat
     2041 aaccggatcc gcgatctgga caactataac aaggcgctgt ttgacgccct gacccacgcg
     2101 ggggtgtggg aagacgacag tcaggtgaaa agaatgctgg tggagtgggg accggttatc
     2161 ccggaaggga aggtcgagat cactatcagt aagtacgaga aaacggcggg tgcagccgcc
     2221 tgatcaagag gagaaacgaa gtatgaataa tctgatggtc attgatggta ttgaagttcg
     2281 tcgtgatgct tatggtcgtt acagcctgaa cgatcttcac agggctgccg gttctctgga
     2341 taagcataag cctgcattct ggctccgcaa tgagcaaact gaacgtttaa taagcgagtt
     2401 gcagatttgc aactcggtca atatagagcc agttaacgtt attcgtggcg gaaataacca
     2461 ggggacgtat gtctgcaaag aactggtgta tgcctatgca atgtggataa gcccgtcatt
     2521 ccatcttaag gtgatccgta ctttcgacat ggtaaccagc gcaccggaaa aattatccgg
     2581 acaggctgct gacaagatgc aggctggtgt gattctgctg gactttatgc gccgggaatt
     2641 aaatctgtct aactcttcag tgcttggtgc ctgtcagaaa ctccaggagg ctgttggctt
     2701 accgaatctg gcaccgcgct atgccattga tgcacctgct gacgcgcctg atggctcaag
     2761 ccgcccgacg ctgtcgctga gtgcactgct gaagcagtat ggtatccgcc tgacggctaa
     2821 tcaggcatat caccagatgg cgaagctggg gatcgttgaa caacgcgaac gatacagccg
     2881 taccgcgatt aacaacatca aaaaattctg gtcgctgaca gcgaaaggct gcatgttcgg
     2941 caagaacatc accagtcccg caaatccgcg cgagacgcag ccgcatttct tcgaatcccg
     3001 attccctgag ctgttaaagc tgctcgatac cgttcattga ggtgaccgtg agagcactac
     3061 tgactcctga aattgccccg cgtatgggga tcgtattgtt caggccaggt tcagagctga
     3121 tgcccctgtt tatgcagggg cgtgtcctgc tggagcctga gccggaacgt tattcatctt
     3181 tcgccagtgg tgccgttccg gcggcatcac aaccgctggc ggatgatcct gccgttcggg
     3241 ccgtgttccg caatgaggca gtgatccgtc gtgctggtgg cgtggaatgt cttgaaagct
     3301 ggttacttcg tgaaaaaggc tgccagtggc ctcattccga ctggcacagc gagaacatga
     3361 ccacaatgcg acacgctccg ggcgcaatcc gtctgtgctg gcactgcgat aaccagctgc
     3421 gcgatcagtt cacggaacgg ctggaatcaa tggcaacgga taactgtgcc cgctgggtgt
     3481 tgtctgttgt gcgtcgggat ctcggttttg atgacagtca cgttgtgaca atgccggaac
     3541 tgtgctggtg gctgattcgt aatgacctgg cggatgcctt accggaaagt gcagcccgta
     3601 aggcacttag attaccgaag cctgttgtgc cgtctgttac ccgggaaagt gaccttgtgc
     3661 cttcggttcc tgccaccagc atcatccagg ataaagcgaa aaaggtgctg gcgctgaaag
     3721 tggatcctga gtcgccggag tcttttatgt tacgcccaaa acgtcgccgc tgggttaatg
     3781 aaaagtacac gcgctgggtt aagacacagc cgtgtgcatg ttgtggaaag cctgctgatg
     3841 atccccacca cctgataggt cacggtcagg gaggaatggg tacaaaagcg catgacctct
     3901 ttgtgttgc
//
LOCUS       DACAPB010000283         3907 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.288, whole genome shotgun sequence.
ACCESSION   DACAPB010000283 DACAPB010000000
VERSION     DACAPB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.288"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..2297
                     /gene="fdnG"
                     /locus_tag="IT904_004516"
     CDS             <1..2297
                     /gene="fdnG"
                     /locus_tag="IT904_004516"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP3194191.1"
                     /translation="DATLIVVDPRFTRTASVADIYAPIRSGTDITFLSGVLRYLIENN
                     KINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYDKSSWNYQFDENGYAKRDET
                     LTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCEVLASTSAPDRTTTFLYALG
                     WTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSTSLPGYLT
                     LPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSLMKSFYGDAAQKENNWGYDW
                     LPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDKNKVVSCLSKLKYMVVIDPL
                     VTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDGSIANSGRWLQWHWKGQDAP
                     GEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNYKQPHEPQSDEVAKENNGYA
                     LEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYTGSWTEQGNQMANRDNSDPS
                     GLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLIQWNGSKWTGNDIPDFGNAA
                     PGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETPLGTNPLHPNVVSNPVVRLY
                     EQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIAQPEQFVEISETLAAAKGIN
                     NGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGIPIHWGFEGVARKGYIANTL
                     TPNVGDANSQTPEYKAFLVNIEKA"
     gene            2310..3194
                     /gene="fdxH"
                     /locus_tag="IT904_004517"
     CDS             2310..3194
                     /gene="fdxH"
                     /locus_tag="IT904_004517"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP3194192.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            3187..3840
                     /gene="fdnI"
                     /locus_tag="IT904_004518"
     CDS             3187..3840
                     /gene="fdnI"
                     /locus_tag="IT904_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAP3194193.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
BASE COUNT          944 a          968 c         1115 g          880 t
ORIGIN      
        1 acgatgcaac cttgatcgtt gtcgatcccc gttttacgcg taccgcttct gtagcggata
       61 tttacgcacc tattcgttcc ggtacggaca ttacgttcct gtctggcgtt ttgcgctacc
      121 tgatcgaaaa caacaaaatc aacgccgaat acgttaagca ttacaccaac gccagcttgc
      181 tggtgcgtga tgattttgct ttcgaagacg gtctgttcag cggctacgac gctgaaaaac
      241 ggcaatacga taaatcgtcc tggaactatc agttcgatga aaacggctat gcgaaacgcg
      301 atgaaacact gactcatccg cgctgtgtgt ggaacctgct gaaagcgcac gtttcccgct
      361 acacgccgga cgttgttgaa aacatctgtg gtacgccaaa agccgacttc ctgaaagtgt
      421 gtgaagtgct ggcctccacc agcgcaccgg atcgcacaac caccttcctg tacgcgctgg
      481 gctggacgca gcacaccgtg ggtgcgcaga acatccgtac tatggcgatg atccagttgc
      541 tgctcggtaa catgggtatg gccggtggcg gtgtgaacgc attgcgtggt cactccaaca
      601 ttcagggtct gactgactta ggtctgctct ctaccagcct gccaggttat ctgacgctgc
      661 cgtcagaaaa acaggttgat ttgcagtcgt atctggaagc gaacacgcct aaagcgacgc
      721 tggctgatca ggtgaactac tggagcaact atccgaagtt cttcgttagc ctgatgaaat
      781 ctttctatgg cgatgccgcg cagaaagaga acaactgggg ctatgactgg ctgccgaagt
      841 gggaccagac ctacgacgtc atcaagtatt tcaacatgat ggacgaaggc aaagtcaccg
      901 gttatttctg ccagggcttt aacccggttg cgtccttccc ggataaaaac aaagtggtga
      961 gctgcctgag caaactgaag tacatggtgg ttatcgatcc gctggtgact gaaacctcta
     1021 ccttctggca gaaccacggc gagtcgaacg atgtcgatcc ggcgtctatt cagactgaag
     1081 tattccgtct gccttcgacc tgctttgccg aagaagatgg ttctatcgct aactccggtc
     1141 gctggttgca gtggcactgg aaaggtcagg acgcgccggg cgaagcgcgt aacgacggcg
     1201 aaattctggc gggtatctac catcatctgc gcgagctgta ccaggccgaa ggtggtaaag
     1261 gcgtagaacc gctgatgaag atgagctgga actacaagca gccgcacgaa ccgcaatctg
     1321 acgaagtggc aaaagagaac aacggctatg cgctggaaga tctctatgac gcaaatggcg
     1381 tgctgattgc gaagaaaggt cagttgctga gtagctttgc gcatctgcgt gatgacggta
     1441 caaccgcatc ttcttgctgg atctacaccg gtagctggac agagcagggc aaccagatgg
     1501 ctaaccgcga taactccgac ccgtccggtc tggggaatac gcttggatgg gcctgggcgt
     1561 ggccgctcaa ccgtcgcgtg ctgtacaacc gtgcttcggc ggatatcaac ggtaaaccgt
     1621 gggatccgaa acggatgctg atccagtgga acggcagcaa gtggacgggt aacgatattc
     1681 ctgacttcgg caatgccgca ccgggtacgc caaccgggcc gtttatcatg cagccggaag
     1741 ggatgggacg cctgtttgcc atcaacaaaa tggcggaagg tccgttcccg gaacactacg
     1801 agccgattga aacgccgctg ggcactaacc cgctgcatcc gaacgtggtg tctaacccgg
     1861 ttgttcgtct gtatgaacaa gacgcgctgc ggatgggtaa aaaagagcag ttcccgtatg
     1921 tgggtacgac ctatcgtctg accgagcact tccacacctg gaccaagcac gcattgctca
     1981 acgcaattgc tcagccggaa cagtttgtgg aaatcagcga aacgctggcg gcggcgaaag
     2041 gcattaataa tggcgatcgt gtcactgtct caagcaaacg tggctttatc cgcgcggtgg
     2101 cagtggtaac gcgccgtctg aagccgctga atgtaaacgg tcagcaggtt gaaacggtgg
     2161 gtattccaat ccactggggc tttgagggtg tcgcgcgtaa aggttatatc gctaacactc
     2221 tgacgccgaa tgtcggtgat gcaaactcgc aaacgccgga atataaagcg ttcttagtca
     2281 acatcgagaa ggcgtaaggg ggcgaacaga tggctatgga aacgcaggac attatcaaaa
     2341 ggtccgcaac taactccatc acgccgcctt ctcaggtgcg tgattacaaa gcagaagtcg
     2401 caaaacttat cgacgtttcc acctgtatcg gttgtaaagc ctgtcaggtg gcgtgttcgg
     2461 agtggaacga catccgtgat gaagtggggc actgcgtcgg ggtttacgat aaccccgccg
     2521 atctgagcgc caagtcctgg acggtgatgc gctttagcga aaccgaacag aacggcaagc
     2581 tggagtggct gatccgtaaa gacggctgta tgcactgtga agatccgggc tgccttaagg
     2641 cgtgcccgtc tgctggtgca atcattcagt acgctaacgg gattgtcgat ttccagtcgg
     2701 aaaactgcat cggctgtggt tactgcattg ccgggtgtcc gtttaatatt ccgcgcctca
     2761 acaaagagga taaccgggta tataaatgca cgctctgcgt cgatcgcgtc agcgtcggcc
     2821 aggaaccggc ttgtgtgaaa acctgtccga ccggggctat ccacttcggc accaagaagg
     2881 agatgctgga gctggcggaa cagcgcgtgg cgaaactgaa agcgcgtggt tacgaacatg
     2941 ctggcgtcta caacccggaa ggggtcggtg gtacgcacgt tatgtacgtg ctgcatcacg
     3001 ccgatcagcc ggagctgtat cacggtctgc cgaaagatcc gaagatcgac acctcggtaa
     3061 gcctgtggaa aggcgcgttg aaaccgctgg cagcggctgg ctttattgcc acttttgccg
     3121 ggttgatttt ccactacatc ggtattggcc cgaataagga agtggacgat gacgaggagg
     3181 atcatcatga gtaagtcgaa aatgattgtg cgcaccaaat ttattgatcg cgcctgtcac
     3241 tggaccgtgg tgatttgctt cttcctggtg gcgctgtccg ggatttcgtt cttcttcccg
     3301 acgctgcaat ggctgacgca aaccttcggt acgccgcaga tgggacgcat tttgcacccg
     3361 ttcttcggca ttgcgatttt cgtcgcactg atgtttatgt ttgtgcgttt tgtgcatcac
     3421 aacatcccgg ataagaaaga tattccgtgg ctgttgaaca ttgtcgaagt attaaaaggc
     3481 aatgagcaca aagtggcgga tgttggtaag tacaacgccg ggcaaaagat gatgttctgg
     3541 tcgatcatga gcatgatttt cgtgctgctg gtgaccgggg tgattatctg gcgtccgtac
     3601 tttgcgcagt acttcccgat gcaggttgtt cgctacagcc tgctgatcca cgcggctgcc
     3661 ggcatcatcc tgatccacgc catcctgatc catatgtata tggcattttg ggtgaaagga
     3721 tcgattaaag ggatgatcga agggaaggta agtcgtcgct gggcgaagaa acaccatccg
     3781 cgctggtatc gtgaaatcga gaaggcagaa gcgaaaaaag agagtgaaga agggatataa
     3841 tctcttttga actttaagct gaaaatggcg ctgtaaaagg cgccattttc atattgtaga
     3901 caacgta
//
LOCUS       DACAPB010000284         3899 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.289, whole genome shotgun sequence.
ACCESSION   DACAPB010000284 DACAPB010000000
VERSION     DACAPB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.289"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..325)
                     /locus_tag="IT904_004519"
     CDS             complement(<1..325)
                     /locus_tag="IT904_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP3194194.1"
                     /translation="MQDIQQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKV"
     gene            complement(325..663)
                     /locus_tag="IT904_004520"
     CDS             complement(325..663)
                     /locus_tag="IT904_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3194195.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(656..3889)
                     /locus_tag="IT904_004521"
     CDS             complement(656..3889)
                     /locus_tag="IT904_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP3194196.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQQDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVAALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLADIAQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTIATYNQIKALQDEGNQQEAARIATEAYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGALQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKSQYSKEEYNNLIAQINERYKDPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQTELTVKLSSTEQELVKWRQQIANLESRPSSKLTQDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEIAAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEREYNNQRLQLERDYADKSRGMSNHVFQEKMQALNDALEREKDIVRQKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLNMYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLSG"
BASE COUNT          933 a         1007 c          767 g         1192 t
ORIGIN      
        1 aaaccttacg ccggaccacc gttccgccga ccagactctg cagatcttcc gccatcccgg
       61 tgaccatacc gtacaggtta gaaaccgtca gcgtggggcg cgtactggtg cctttgccat
      121 tcagttcaaa accactcccc tgaatgggat acggctgata ctgtcgcccc tgccaggtga
      181 ccggctcacc tttttcgttc tgctcattac agaaaaaata acgttctcca ccgacctctg
      241 tcaggtcgat ttcccagagc accacgctgg ccgactgctc cgcacgggtg cattcattca
      301 gtgtttcctg ttgtatgtcc tgcatcagat caccacttcg tcaaactgac acgaaaaatc
      361 ggtataggtg atatgttctg tcgctgacca cgttcgacac accactctga tttttctgtt
      421 aatacccggc gggcgccaaa aaaaagattt atagccttca tgccgggcta aaaatttttc
      481 aaatgaatca cgttcattcg cctcaacttt gaaatcacag gtgaaaacgc gcagagaatg
      541 attaagtcca tttggacttc gttgctcata accatcgcca aatcttacag tttttatcga
      601 tggtttattt tctgttttca ttccatcctc tggtaaccag tgaaattctt ccgtattagc
      661 cacttaacat tcccccatca cgtcgcatat ttaacagatt gccctgcacc cgctgatcaa
      721 ccatccccat aagtgctttt attgcctgag ggccaatctc tccattctgg ccgtcatttt
      781 gaatagtgat ttggtataca ggggcatagg taatatctcc gcctccatta ccacttttac
      841 tattaatagc tctgacacca agagaaccat cggaagttcg tgttaatggc ataatggctt
      901 ccggaccagc ctcaccaaaa acaccagccc cttttgcaaa agcaaaaaat tgcggggaat
      961 cataaatacc gttcgaatac atgttcaatg acggagactc ataaacaccg ccctttgcat
     1021 tcggaataaa tttactaata gcactcccga tagttcctaa aatcccccca gaagaactgt
     1081 tactaatgct gtcgaaaatc ccagtaattg aagcctttaa tgctattcga ctaagatccg
     1141 aaatcacgga agtagcgaaa gaacgaaaat ttgccttgcc tgtcgtgaca aaatcaccca
     1201 gcgcatcggt cattccatca aacatctgag tcgtggttga ttttatctgc tcactgatat
     1261 ccttagtgtc atccagccag ttattgaatc cctgggaggc accactaacc cagtctcctg
     1321 cctgaatatc gagctgctcg tttttctgtc tgacaatgtc tttttctcgt tccagagcat
     1381 cattcagagc ctgcattttc tcctgaaaaa catgatttga catcccacgg gatttatctg
     1441 catagtcacg ttcaagttgc agacgctgat tgttatattc acgttcaatc cgcagtaatt
     1501 cctgctggcg ttgctgattt ttatcgccaa ccccataacc agcaatctga atatcatacc
     1561 cctgttgacg attatcaatc gaagcctgca atgaatcacg ccatgcagct atttcggcgg
     1621 attccttgat tagcctgtta tttttttcaa tcgcaacatt tttctccatc aacgcggtta
     1681 tttcttcccg gtgtaagaga agcgatttct gatcctgggt taatttcgat gacggtcgtg
     1741 actccaggtt ggcaatctgc tgacgccact taaccagttc ctgttcagtg gaacttaatt
     1801 taacggttaa ttcagtttgt gaacttagta acgcattctg ttgattcaga tgatcaatca
     1861 ttcgttgggc agcatcatcc gaataacctt tcgcctttgg ttgctttgga tctttataac
     1921 gctcatttat ttgagcgata agattattat attcttcctt tgaatactga ctctttagct
     1981 tttccagttt tgcaagttca ctagctctct gctgctcccg ggtctgatat tgttttgaaa
     2041 aggcatctgc tctctgtctg agttcaattc cttcctgttg tcgcttgttg taatcgttta
     2101 ttgatgaatt tagtacgtcc tgagcaattt tttctgcttg aagtgcgccc aattgctgct
     2161 ttaatcgtgc cagcctttta ttttgagctc cgccatctcc aatgccacct aacccgaaaa
     2221 ctccgggtcg ggtatttttt tctatttcat caatttgacg gagaacatct gagatttttt
     2281 gatcaaggga ggcctcacgg ccaatatcca gcatggaatc ccatgcccat tttgcggagt
     2341 ctgcgacagc tttccatgca gtctcaagat aaccaagatt ttctttaatc tggttggtgc
     2401 gctggatcat tgaggatgag tatgcttctg tcgcaatgcg ggcggcctcc tgctggttcc
     2461 cttcatcctg tagcgcctta atctggttat aagtcgcaat tgtcagaaaa tggtactgat
     2521 cattaagttt tgatatggca ctgacaggat cctttgcaat ttcattgaag tcattaacca
     2581 gttgttcggt tgatattcct gttacttcgc tcgtttttac tatcgctgtc gtcacttgct
     2641 ccagcgaact gctcgctacc tttcccgaac gcacaagctg gtttaatact gccgccgcag
     2701 cgccagttgt cgaatcagcc gcattcccgg cacgttgagc tatatcggcc aattgcccgc
     2761 tggttgtccc caactgattt ccggtaagaa taagagattt attaaattcg tcctgctcct
     2821 gagagccttt atagtaagcc agccctaaga cgccaacggc tgctgcggcc agggtaaaag
     2881 gattaattaa tcccagcaca taagaaccaa cacctttgat cgccgggcca atcccaccga
     2941 acatatcttt tagctggccg ccctgctgca ttaacaccat aaaaggcgac tgaccagtgg
     3001 acaacccaac aacaatatcc gtcatttgtg caggcaacat gcgcatagca aaagccgttt
     3061 gttttgccga cattccggtt ttgcttaatt gagattgagt aacctcaagc tcactccgca
     3121 tagcacgaag ttttccagaa agttcctcat acatttcagg agagagcatc cccttagctt
     3181 ttgcttcatt gagctgtttc tgttgttcta ccagacgatt aaaagcagtt ccgacagggt
     3241 caagttgagc aatcagacgt tgcaaagcag caacctgttc atcatgcgct tttgctgctt
     3301 ctcgctctgc ctgagcctct ccggtaagct ctcgccgtgt ttcctgtatt tttcggctat
     3361 aattctcaaa ctgagaacca tttattttcc cggatgcaaa cgcagcatta agttcatcct
     3421 gctgttgttc aagatttctt agcgccgcag ccagagggtc gatcttgtcc agcattcttt
     3481 gaaaggcctg agcctgcgct tcctgctggg cggcagcaag ttttccggcc ttctcggctt
     3541 ctctctgcgc ttgcgcaacc ccgctcaatt cctctgtggt ttcattaagt ttacggacaa
     3601 gaaattcata ttcttcttta tcaataagcc ctttatcgaa aaatttcttt aattcagaat
     3661 agcgtcgacc gacagtatca attgcggcac caactggatc aatagctgct tttaattttg
     3721 cgagcgcgtt cttctcatct tctgttgcct tagtcacttt ccctgcgcta tttgcagcag
     3781 tttccccggc ctgcgtcatt ttgactaatg aggaggtcag attgtcagca ttattttttg
     3841 ctccagtgct atcaataatt attgcgagac gcgaggtttg ctctgccatt tattaaaac
//
LOCUS       DACAPB010000285         3866 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.290, whole genome shotgun sequence.
ACCESSION   DACAPB010000285 DACAPB010000000
VERSION     DACAPB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.290"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            220..1374
                     /locus_tag="IT904_004522"
     CDS             220..1374
                     /locus_tag="IT904_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HAP3194197.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            1387..2247
                     /gene="kbaY"
                     /locus_tag="IT904_004523"
     CDS             1387..2247
                     /gene="kbaY"
                     /locus_tag="IT904_004523"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HAP3194198.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            2414..2890
                     /gene="agaB"
                     /locus_tag="IT904_004524"
     CDS             2414..2890
                     /gene="agaB"
                     /locus_tag="IT904_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP3194199.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            2929..3732
                     /locus_tag="IT904_004525"
     CDS             2929..3732
                     /locus_tag="IT904_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine permease IIC component 1"
                     /protein_id="HAP3194200.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            3722..>3866
                     /locus_tag="IT904_004526"
     CDS             3722..>3866
                     /locus_tag="IT904_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417609.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IID with IIC forms the translocation channel;
                     involved in N-acetylgalactosamine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAP3194201.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKD"
BASE COUNT          956 a          973 c          960 g          977 t
ORIGIN      
        1 tcggtgaaca tagcgacgtc attacgcaat tctttaatct tcccttgtct tttctttcaa
       61 tgatcacaga tttcgtttta tttccttttt ctccattgaa ctttcagttt cttttctata
      121 gattttaatc aacgaaagac atcaccaagt gaaatgaaac gaaaggcaag tgaaagcgac
      181 aacgcccgac gtcaagttca tcagactaag gattgagtta tgccagaaaa ttacacccct
      241 gctgccgccg caaccggtac atggactgaa gaagagatcc gccatcagcc tcgcgcatgg
      301 atccgttcac tcaccaacat cgacgcgcta cgttccgcgc tcaataactt ccttgaaccg
      361 ttactgcgca aagagaatct gcggatcatc ctgaccggag ccggaacgtc ggcatttatc
      421 ggtgacatca tcgcgccgtg gctcgccagc cataccggta aaaacttcag cgccgtaccg
      481 accaccgatc tggtcaccaa tccgatggac tacctgaacc cagctcatcc gctgctgttg
      541 atctccttcg gtcgatccgg caacagcccg gaaagcgtcg cagccgtgga actggcaaat
      601 caatttgtac cggaatgcta tcacctgccg atcacctgca acgaagcggg cgctctttac
      661 caaaacgcga tcaacagcga taacgcgttt gccctgctga tgcccgcaga aacgcacgat
      721 cgcggctttg cgatgaccag cagcattacc accatgatgg ccagctgcct cgcggttttc
      781 gcacctgaga cgatcaacag ccaaaccttc cgcgacgtgg cggatcgttg ccaggcgatc
      841 ctgacctcac tgggcgattt cagcgaaggt gtgtttggtt acgcaccgtg gaaacggatc
      901 gtttatctcg gtagcggtgg cttacagggc gcagcacgcg agtcggcgct gaaagtgctg
      961 gaactgacgg cgggtaaact ggcggccttt tatgattctc caaccggatt ccgtcatgga
     1021 ccaaaatcgc tggtcgatga cgaaacgctg gtggtggtat ttgtctccag ccacccttac
     1081 acccgtcagt atgatcttga tctgctggct gaacttcgcc gtgacaacca ggcaatgcgt
     1141 gtaatcgcca tcgccgcgga aagcagcgac atcgtcgctg ccggtccaca tatcatcctg
     1201 ccaccgtcac gtcactttat cgacgttgag caggcatttt gcttcctgat gtacgcccag
     1261 acgtttgcac tgatgcagtc gctgcacatg ggcaatacgc cggatacccc atcagccagt
     1321 ggcaccgtta accgcgtggt gcaaggcgta atcattcatc cgtggcaggc ataagaggat
     1381 cgcattatga gcattatctc cactaaatat ctgttacagg acgcccaggc caatggctac
     1441 gcggtgcctg cttttaacat tcataacgcc gagacgatcc aggcgatcct cgaagtgtgc
     1501 agtgaaatgc gatcgccggt gatcctcgcc ggaacgccgg ggacctttaa acacatcgcg
     1561 ctggaagaga tctacgccct gtgtagcgcc tattccacaa cctacaacat gccactggcg
     1621 ctgcatctcg accaccacga atcgctggat gatattcgcc gtaaagtcca cgcaggtgtg
     1681 cgcagtgcga tgatcgacgg cagccacttc ccgtttgccg agaacgtgaa gctggtgaaa
     1741 tcggttgttg acttctgcca ctcacaagat tgcagcgtgg aagcagaact gggccgcctg
     1801 ggcggtgttg aagatgacat gagcgttgac gccgaaagtg cattcctgac cgatccacaa
     1861 gaagctaaac gctttgtcga actgactggc gtcgacagcc tggcggtagc gattggtacg
     1921 gcgcacggct tatacagcaa aacgccgaag attgatttcc agcggctggc ggaaattcgt
     1981 gaagtggtgg atgttcctct ggtgctgcat ggtgccagcg atgttccgga tgaatttgtc
     2041 cgtcgcacta ttgaacttgg cgtcacaaaa gtgaacgttg ccacagaatt aaaaatagcc
     2101 ttcgctggcg cggttaaagc ctggtttgcg gaaaatccgc agggtaatga tcctcgttat
     2161 tatatgcgcg tcggaatgga tgcgatgaaa gaagttgtca gaaataaaat taatgtctgt
     2221 ggttcagcga atcgaatttc agcataatca ttagtttttt atgatttatc ccaatgtact
     2281 tcccgattaa tatcggggag tgccttaatg gaaaaggaga taactaaacc ttaataaata
     2341 catcactaca atatcgcaac aataatatat ttaaaaaaat tatattattc aactttatgg
     2401 tgaggattac acaatgagca gtccaaatat tctcttaacc cgtattgata accgtctggt
     2461 tcacggtcag gttggcgtga cctggacatc caccatcggt gcaaatctgc tggtagtcgt
     2521 ggatgatgtt gtcgctaacg atgatattca acagaaatta atgggtatta ccgcggaaac
     2581 ctacggcttt ggcattcgtt tctttactat cgaaaaaacc attaacgtca tcggcaaagc
     2641 tgcaccacat cagaagatct tcctgatttg ccgtacgcca caaacggtac gtaaattggt
     2701 agaaggtggt attgacctga aagatgtcaa cgtcggcaat atgcatttct cggaagggaa
     2761 aaagcaaatc agcagtaaag tttatgtcga tgaccaggat ctcacggact tacgttttat
     2821 taaacaacgt ggcgtgaatg ttttcattca ggacgtccct ggcgatcaaa aagaacaaat
     2881 ccctgactaa atctaaaatc gccttaatat tggtttgagg taataaaaat gcatgaaata
     2941 accctacttc agggattatc cctggcggcg ttagtttttg ttctggggat tgatttttgg
     3001 ctggaagcct tatttttatt ccgcccgata atcgtttgta ccctaactgg cgctattctc
     3061 ggtgatattc agactggctt aattaccggt ggtctgacag agttggcttt cgccggatta
     3121 acccctgcag gtggtgttca gccgcccaac ccgattatgg cgggtctgat gaccaccgtc
     3181 attgcatggt ctacgggcgt tgatgccaaa acagcaattg gtcttggcct gccgtttagt
     3241 ttgttaatgc agtacgtcat tctgttcttc tattccgctt tctcattatt tatgaccaaa
     3301 gccgataaat gcgcgaaaga ggcggatacg gcagcgtttt cccggcttaa ctggacaacg
     3361 atgctcatcg tcgcttcagc gtatgcggtg attgctttcc tctgtactta cctggcacag
     3421 ggggcgatgc aggcgctggt gaaagcgatg cccgcctggc tgacccacgg ctttgaagtg
     3481 gcaggcggta ttctgcctgc cgttggtttt ggcttgctgc tgcgcgtaat gttcaaagcg
     3541 caatatatcc cttacctgat cgccggtttc ctgtttgttt gctacatcca ggtcagcaac
     3601 ctgttgccgg ttgccgtact gggcgcaggc tttgcggtgt atgagttttt caatgcgaaa
     3661 tcccggcagc aagcgcaacc gcagcccgtt gccagtaaaa atgaagaaga ggactacagc
     3721 aatgggatct gaaatcagta aaaaagatat cacccgtctg ggctttcgtt cgtcgctgct
     3781 gcaagcgagc tttaactacg aaaggatgca ggcgggcggt tttacttggg cgatgttgcc
     3841 gatcctgaaa aagatttata aggacg
//
LOCUS       DACAPB010000286         3851 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.291, whole genome shotgun sequence.
ACCESSION   DACAPB010000286 DACAPB010000000
VERSION     DACAPB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.291"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..423)
                     /gene="guaA"
                     /locus_tag="IT904_004527"
     CDS             complement(<1..423)
                     /gene="guaA"
                     /locus_tag="IT904_004527"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP3194202.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWM"
     gene            complement(492..1958)
                     /gene="guaB"
                     /locus_tag="IT904_004528"
     CDS             complement(492..1958)
                     /gene="guaB"
                     /locus_tag="IT904_004528"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP3194203.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            2120..3490
                     /gene="xseA"
                     /locus_tag="IT904_004529"
     CDS             2120..3490
                     /gene="xseA"
                     /locus_tag="IT904_004529"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP3194204.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLKKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(3487..3702)
                     /locus_tag="IT904_004530"
     CDS             complement(3487..3702)
                     /locus_tag="IT904_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194205.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
BASE COUNT          940 a         1056 c          973 g          882 t
ORIGIN      
        1 catccagaca tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac
       61 cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt cgttagaggc
      121 ttcaacgtga ccgcccaact gcatcgccat ggtctgcata ccatagcaaa cgccgaatac
      181 cggtacgcct gcttcaaaga catactgcgg cgcacgcgga ctgttttctt cagtagtact
      241 ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt gtgcttctgt
      301 cacatcccac gcccacagtt cgcagtaaac gcccagctca cgcacgcggc gcgcaaccag
      361 ttgagtatac tgggaaccga agtccagaat gaggatgcga tgcttatgaa tgttttccgt
      421 cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga catgaagtcg
      481 ggcgaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat ggtcacgtcg
      541 tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc tttagtacgc
      601 agttcgtcga tagtaccaca gccggtcagg cccatacagg agcgcaggcc acccatctgc
      661 tggtgaatga tctctttcag gcgaccttta taagctacgc gaccttcgat accttccggc
      721 accagtttgt cggcagcgtt atcgctctgg aaataacggt cagaggaacc tttggacatc
      781 gcgcccaggg aacccatacc acggtaagat ttgtaagaac ggccctggta gagttcgatt
      841 tcacccggag attcttcagt acccgccagc atggaaccca ccattaccgc gcttgcgcca
      901 gcggcgatag ctttggcgat gtcgccggag aagcgaatac caccatctgc gataaccgga
      961 atacctgtgc cttccagcgc ttcaactgcg tcagcaacgg cagtgatctg cggaacacct
     1021 acgccagtaa cgatacgagt agtacagata gaaccagggc cgataccgac tttaaccgcg
     1081 ctgcaaccag cttctgccaa agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg
     1141 atttgcagat ccggatattt agcacgggtt tcacggatac gctgcaaaac gccttctgag
     1201 tgaccgtggg aggagtcgat cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca
     1261 cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa cacgcagacg gccttgctcg
     1321 tctttacagg cgttcggttt acgttccgct ttctggaaat ctttcacggt gatcatgccg
     1381 atcaggtgga attcgtcatc aaccaccagc gctttttcaa cgcgtttttc gtgcattttt
     1441 gccagcacca cttcacgggc ttcaccttca cgcacggtga ccagacgctc tttcggcgtc
     1501 atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg accggtgata
     1561 atacccacca gttcgttttc ttcggtaacg accggatagc ccgcaaaacc gttacgctcg
     1621 gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc
     1681 acaccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc aatggacatg
     1741 tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag gcgcgcttcc
     1801 gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt tttcgtcagc
     1861 tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac gaggagaacg
     1921 tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc gaccagagtg
     1981 gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta cccctttttg
     2041 caaaaaatgc ttgctatccc cggagggcgg gttactatcg actgaataac ctgctgattt
     2101 agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta ccgttagtcg
     2161 cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt ggatcagcgg
     2221 cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac tcaaagacga
     2281 caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga ccttccgccc
     2341 acagcatggg caacaagttt tagttcgcgc caatattacg ctctacgagc cgcgcggcga
     2401 ctaccagata atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc aacagaagta
     2461 cgaacagctc aaagcgaagt tgcaggctga aggtttgttc gatcagcaat acaaaaaacc
     2521 acttccctcc cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca
     2581 tgatattttg catgtgttaa aacgtcgcga tccttctcta ccggtgatca tctaccccac
     2641 cgccgttcag ggcgatgacg cgccggggca aattgttcgc gccattgaac tggcgaatca
     2701 gcgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg
     2761 gagttttaac gacgaacgcg tagcgcgggc tatttttgcc agccgcattc cggtcgtcag
     2821 cgccgtcggg catgagacgg atgtgaccat tgccgatttt gttgccgatc tgcgtgcgcc
     2881 aacgccgtct gccgccgctg aagtagtaag ccgtaatcag caagagttac tgcgccaggt
     2941 gcaatcggcc cgtcaacggc tggagatggc gatggattat tatctcgcca accgtacgcg
     3001 tcgctttacg cagattcatc atcgcttgca gcagcagcat ccgcagctcc ggctggcacg
     3061 ccagcaaacc atgcttgaac gcctgaaaaa acggatgagc tttgcgctgg aaaatcagct
     3121 taagcgtgcc gggcaacagc agcagcgatt aacacagcgg ctgaatcagc aaaatccaca
     3181 gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt tagcagaaat
     3241 cctgcgcgca cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga
     3301 agccgtaagc ccactgtcaa cgctggcgcg tggatacagc gttactactg ctactgacgg
     3361 caatgtactg aaaaaagtga agcaagttaa agcgggtgaa atgctaacca cacgtctgga
     3421 agacggctgg atagaaagtg aagtaaaaaa catccagccg gtaaaaaaat cgcgtaaaaa
     3481 ggttcattaa gccagcacga attcaacgcg ttttttcgat atcaagccat gcccgtgctg
     3541 acagaagtaa tctactgcac cgcaggcttt taacacctgc aacggttgat ggcagtctgg
     3601 acaaagagct ttcatttcaa taacttcccc gcaggatggg caatgcgcat ggccgttatc
     3661 ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattc ctccggtagc
     3721 cacccctcta cattcataga gggaatggca gataaaatac ttacggataa ttatttattt
     3781 ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt cagggtgtta
     3841 cgcttgttcg c
//
LOCUS       DACAPB010000287         3848 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.292, whole genome shotgun sequence.
ACCESSION   DACAPB010000287 DACAPB010000000
VERSION     DACAPB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.292"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            20..418
                     /locus_tag="IT904_004531"
     CDS             20..418
                     /locus_tag="IT904_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP3194206.1"
                     /translation="MAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYDDSHPEALAR
                     NTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPLLVARMRSHSSAYFRELVETRERLV
                     RDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            490..1560
                     /locus_tag="IT904_004532"
     CDS             490..1560
                     /locus_tag="IT904_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP3194207.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGESRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVTPGQPAGTTPEADSAYALKADSGAVQQVMTARQEQSHQL
                     QQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            1601..2023
                     /locus_tag="IT904_004533"
     CDS             1601..2023
                     /locus_tag="IT904_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP3194208.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEAMQRVLAFYQGNFQEAVL"
     gene            2024..2437
                     /locus_tag="IT904_004534"
     CDS             2024..2437
                     /locus_tag="IT904_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194209.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKREIRLADDVRL
                     LRQEERERRKNIEKLIQIERNAKARIRRATKSRTE"
     gene            2531..2713
                     /locus_tag="IT904_004535"
     CDS             2531..2713
                     /locus_tag="IT904_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194210.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            3327..3545
                     /locus_tag="IT904_004536"
     CDS             3327..3545
                     /locus_tag="IT904_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194211.1"
                     /translation="MFKCNCTLIVGGIMKNALQFLFVAFWLFVSCMPIIFTARYMEKV
                     DVLILIFGYINALFLGVFMAVMCIEYWR"
BASE COUNT         1139 a          768 c         1061 g          880 t
ORIGIN      
        1 caagcatgaa cacatccgca tggcgatgaa tgcctgggcg catccggacg gcgaaaaagt
       61 accggctgcg aaaattacca aagcgtattt cgagctggga atgacgttcc cggaactgta
      121 tgacgacagc catccggaag ccctggctcg caatactcag aaaattttcc gctgggtgga
      181 gaaagacacc cctgatgcgg ttaaaaaaat tcaggcgttg ttaccagcta tcgaaaaagc
      241 aatgccacct ctgctggtgg cccgaatgcg cagtcatagc tcagcctatt ttcgggaact
      301 agtggagacg cgggaacgac tggtgagaga cgctgatgat tttgtcgcag tggcgatcgc
      361 tggtttcaac cagatgaatc gtggtggccc tgcaggaaat attgtggctg tgcattgact
      421 cgcaatattc ataccggatc acttccggca atttgtgagt aaaaagattc ggtatcaaaa
      481 gaggtgagta tggctaacgc ctggctcaga ttatggcatg acatgccaaa tgaccctaag
      541 tggcgaacaa ttgccagggt gtcagggcag ccaattgcaa cagtgatggc agtgtatatc
      601 cacctcttgg tgagcgcgtc acgaaatgtc acgcgaggtc acattgatgt cacgacagaa
      661 gatttggcaa gtgcgctcga cgtgacagaa gaggtgattg attcaatttt gcagacgatg
      721 caggggcggg tacttgatgg tgatttaatc actggatggg aaaaacgcca ggtgcttaaa
      781 gaggacaacg gcaatatttc gcaaaccgca aaatctcctg cagagcgcaa gagggcgcag
      841 cgagagaggg aaagaaagcg ggaacaaaat ggcgattgtc acggcgagtc acgaaatgtc
      901 acgcacatgt cacgacgagt cacgacagat aaagatacag ataaagatac agatcaagaa
      961 gatcaaaaca ctatggtcca tggcgtaaaa aacgccacga accaggcagg ggatgttcag
     1021 accgtcactc ctggccagcc cgcaggcacg acaccggaag ccgattcagc gtatgcgctg
     1081 aaagccgatt cgggcgctgt gcagcaggtg atgaccgcaa ggcaggagca atcacaccaa
     1141 cttcagcagc ctgaagccga ttccgccatt cagcgggaag ccgatcgggt agtcccggaa
     1201 aacaccgggc agcctgtggg acgagtggat tatcctgatg tgttcgaaca ggtctggcgg
     1261 gaatacccgt tgcgtgccgg ggcaaacccg aagaaatccg ctttcagtgc ctggaaagcc
     1321 agattacgtg agggggtgcc accagaggcc atgctggatg gcgtgaggcg ttacgcaaga
     1381 taccttgcgg ctaccgggaa aacgggaacg gaatttgttc agcgagcgac gacgtttttt
     1441 ggaccggacc ggaattttga aaacccctgg ctgctcccgg taagcggcac gaacaaccag
     1501 cgttgtgtga atcatatttc tgaaccggat aacgaaattc cgccgggctt cagggggtaa
     1561 gtgttaattt ctggtcatga ggtaattttc aggagggctt gtggcaaaag tttttacaca
     1621 agaagagcgg gaaaaaatta aaggacaggt tcttgaactc gtacgccaga gtgggcgcga
     1681 gacgttacga caactggaag ctaaaactgg ggcaacaaga tatctgatga gcgttctggc
     1741 cagagagctg gttgccagtg gcgatgtata caattctggc tacgggttat tcccgtctga
     1801 acaggctcgt aaagactggc aaaatgcccg caaaaaatta tcgagggcaa agctgaagaa
     1861 accggttgtg gttgatccgg accttatctg gtcgttacct gacggagaaa tacgtcgcta
     1921 cgacagtcgc ctaaacataa tctgtcgcga gtgccggaag agtgaagcta tgcagcgtgt
     1981 actggctttc tatcagggta attttcagga ggcggtactg taagtgaaat tagctatcag
     2041 gcttcaatta ccgctggcat tcgcatcaaa ggagaggagc atggaaataa aaccagagga
     2101 tgagttaagc aatatcgttt tatttccggt aaaagaggat gaccctcgta atcaggttaa
     2161 ttttctttat gagccatcgg aaagaccata ttgtcatcac gcctctgtcc gggttgacga
     2221 aaaagagcgt caggtccgct gtaaaatctg cggtgcagtt gtggagccat ttgactggat
     2281 gctctctgtg gcgaaaagag aaatcagact ggcagatgat gtaaggctct tgcgtcagga
     2341 ggagcgggaa aggcgaaaaa atatagaaaa gctaattcag attgagcgta acgcgaaagc
     2401 gcggatacgc agggcgacaa aatccagaac tgaataatta aatttagctc tgttaaaaat
     2461 ttaatcctta accggaggga tttctgcacc ctcagaacat caggaggccg cccgaaaggg
     2521 cggtagttaa atgcgaaagt ttaaaataat tattgaaacg ggaatagccg gtggagattt
     2581 cgaggatgaa ttcgaagtgg atgatgatgc gacgcctgat gaaatacatg acgaagcaaa
     2641 agatattttc tttaactact gcaattactc atatcacgaa ataaaagacg aagaggaaga
     2701 acaaaatggc tgattttggt tcaactaaat acaacgtcag ttttgaagaa tggcatgaac
     2761 tgttaatgga ctatgcagag ttacgtggtg gcagtgctgc tgatgctgaa gcatggcgtg
     2821 atgattatga agcaggaaaa actccggtcg aagcatattg tgatgagtgg ggcgatgaat
     2881 gagcgaggtt aattatcagg aagggcatga aacggcgggg caagcaaaaa cagtggcatg
     2941 gcgatatcgc tacgtgaaaa aaaaggcgtt acggactttc aggagaagta gtggtctggt
     3001 gactggaaat atgtaccgaa aaaagaggat tgtaacgaca ggccgaacta tgaaattcag
     3061 gccttattca ctgccccgcc agtcccggtt acatcagaag aactggttaa agctgtgcac
     3121 ttttatgaac aactaaaacg cgaaaatcca ccagcatccg gaaacctgat tacagattcc
     3181 cagataaggc aatgagctac ctggcgcaga acgggctgat aagtatgggg aatgttttac
     3241 gatgaatatt tagactaaag agtttgtaac gctatgtaag tgattttttc tggtttagat
     3301 atttatatgt tcggccaaat tgaggtgtgt ttaaatgtaa ttgcacattg attgtaggag
     3361 gaataatgaa aaacgcattg cagtttttgt ttgttgcgtt ctggttgttc gtatcatgta
     3421 tgcccatcat cttcacagca aggtatatgg aaaaagttga tgttttgata ttaatatttg
     3481 gatatataaa tgcccttttt ttaggggtgt tcatggcggt catgtgcatt gaatactggc
     3541 ggtaaataca gcgaacgcca ttggtttagt tggatattta ctgtgccgga caaaaacggt
     3601 ttgcggggaa atcttagtta agtagaatga ctgcgggtgc ttgaggctat ctgtctcagg
     3661 catgaacacc aaaaggcaga tagagaaaag ccccagttaa cattacgcgt cctgcaagac
     3721 gcttaacatt aatctgaggc tcaatctatg aacggcaaat ctaggttagc ctcttacgtg
     3781 ccgaaaggca aggagaagca ggctatgaag cagcaaaagg cgatgttaat cgccctgatc
     3841 gtcatctg
//
LOCUS       DACAPB010000288         3812 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.293, whole genome shotgun sequence.
ACCESSION   DACAPB010000288 DACAPB010000000
VERSION     DACAPB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.293"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..482)
                     /locus_tag="IT904_004537"
     CDS             complement(<1..482)
                     /locus_tag="IT904_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP3194212.1"
                     /translation="MSGLPQGRPTFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKN
                     ITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRRIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     "
     gene            complement(486..1286)
                     /locus_tag="IT904_004538"
     CDS             complement(486..1286)
                     /locus_tag="IT904_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP3194213.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1527..2441
                     /locus_tag="IT904_004539"
     CDS             1527..2441
                     /locus_tag="IT904_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3194214.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPFIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(2438..3241)
                     /gene="dkgB"
                     /locus_tag="IT904_004540"
     CDS             complement(2438..3241)
                     /gene="dkgB"
                     /locus_tag="IT904_004540"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAP3194215.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(3404..3480)
                     /locus_tag="IT904_004541"
     tRNA            complement(3404..3480)
                     /locus_tag="IT904_004541"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3444..3446),aa:Asp,seq:gtc)
     gene            complement(3535..3650)
                     /gene="rrf"
                     /locus_tag="IT904_004542"
     rRNA            complement(3535..3650)
                     /gene="rrf"
                     /locus_tag="IT904_004542"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          971 a          944 c          913 g          984 t
ORIGIN      
        1 attctcacga cccatgaaga aaattccagc ggtaacttat ctgtaattaa attatcaact
       61 atcagattgg cttcattgat taacgtcaac cactcaccgc tggcgtagtt tcgtacgtga
      121 gaggtatcgc gtaatgcttc taccgtctgt aaccagatgt cgcgcactgg gtggcccgga
      181 gacattacgt ccatcacaat ccgcctaccg ccaggtttca atatccggtt cacttcacgc
      241 agtgctgcac caacatcatg ccagtgatgg gcagaataac ggctgataac aatatcaaat
      301 gcgttatcgg caaatggcag actttcggca taaccctggc gggtggtgat atttttcagt
      361 tgccggactt cggcagcttg tgccacgaca tccagcatgt gggcagataa gtcatacgcc
      421 accaccgtgc tcacgttttg cgcagcgaca aacgttggtc tgccctgtgg cagacctgac
      481 ataccttatt tatcaggctt gccgggactg aattcaacga gtagcggatt gtgatcggaa
      541 gcgcgcgtaa ccagtacaga agcttcactg acgttcagac cacggtagaa aacaaaatcg
      601 agcgggcgac caaacgcccg acggcgctga tcatcggtaa aacgcacctg gcgcagcgac
      661 atttcccgcg caaagcgata taacgcgttc atccttctac ggctccaggc attgaaatct
      721 cccgccataa tgaccgggcc gctgtggtga gctatctgat caccaatagg aagtaactgc
      781 ttactataga catccacgcc cagactgaag ttgacggcgt gtatattaac caccatcaac
      841 aggcgggtgt caggtaatgg atagaccgtc accagtgccg acttcgccag acgcaaaatg
      901 ggttctcgtt cacgtaacgg gcagcaatac actggatgtg ccgccgaaag ggtcattacg
      961 ccagaaggat gttgtggcag cacgaaagcg ggtacctgat cggcggcaag atagttagcg
     1021 gtcgcaaact gtactaactc tggcgttgtc tgcgcttcct gcaataacac cagatgtgca
     1081 tctttgccgt agttctttaa taccgacaac cattcagcgc gttgctgttt gtatatgttc
     1141 cacaccagga tccgaatacg ctcttcggta cttaacggtt ccccaggtgg taatgcctgg
     1201 ccgatgctcg caaaagaccc cggcggtaag atcctttccg caggttgtcc ggcaacatag
     1261 cgcatggcat aggtgttttt tcgcactttt gactttgaaa cctctattac gtcaaccctt
     1321 ccgcactcgg gagggttcta ctgttatagg gattttagct cactctttca acgagcaatt
     1381 gtatattgtt atgtaagcaa gtgcttacac cggtcagaat tttgctcatt atggagaaca
     1441 ttaaatcgca tgacatatca cgcatcctga tggtttaatg acaaataaat tcaaaatatc
     1501 tcttctattt tgcaacagga gcaaaaatga aagccacgtc ggaagaactc gccatttttg
     1561 tttcggtcgt cgaaagcggc agctttagcc gggcagcgga acaattaggg caagcaaact
     1621 cagcggtaag ccgggcggtg aaaaagctgg agatgaaact tggcgttagc ctgcttaatc
     1681 ggaccacgcg acaacttagc ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt
     1741 caattttgca ggagatggca gcggcagaat cagaaattat ggagacgcgt aatacaccgc
     1801 gtggactgtt acggatcgat gccgcaactc cagtggtgct gcactttctg atgccgttta
     1861 ttaagccttt ccgtgaacgc tatccggaag tcactttgtc gctagtctcc tccgaaacga
     1921 ttattaattt gatcgaaaga aaagtggatg tcgcgatacg cgctggtacg ttaacagatt
     1981 ccagcttacg tgccaggccg ttatttaaca gttatcgaaa aattatcgcc tcccccgatt
     2041 atatttcccg ctacgggaag ccagaaacga tcgacgattt aaagcaacat gtttgcctgg
     2101 gattcactga acccgcttcc ctcaatacct ggccgatagc ctgtagtgat ggacaattac
     2161 atgaggtgaa gtacggtttg tcatccaata gtggggaaac actgaaacag ctttgcctga
     2221 gtggcaacgg gattgcgtgt ttgtccgact acatgatcga caaagaaatc gctcgcggag
     2281 aattggtgga gttaatggca gataaagtgt tgccagtgga aatgccattc agtgccgtct
     2341 attacagcga ccgtgcggta agtacgcgca tccgggcttt tatcgatttc cttagcgagc
     2401 atgtaaaaac agctcccgga ggagctgtca gagaggctta atcccattca ggagccagac
     2461 cttccgggct aaccaggcgg tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg
     2521 catcaagctg taaattttgt gccttaagat tactttccag gtttttacgt ttagtagaag
     2581 aaggaattac tgagtaacct tcccccatag cccacgccag aatcacttgt gccggagtcg
     2641 cattgtgttt agctgcgata cgagcaataa cctcatcttt cagggcctta ccatacgcca
     2701 gcgtcatata ggaagtaata tggatgccgt gctgtttagc ccaggcaacc actttacggt
     2761 tttgcagata aggagagagt tcaatctggt tagtagcgat gttttcagcg ccaacagcag
     2821 caatcgcctt ttccatcaat gggatcgtga agttggaaat accgatctca cgcgtcaacc
     2881 cttctttttt ggcttccagc agcgcctgca taaactcttc aacagagact tcatcgtttg
     2941 gtgacggcca gtggattaga gtcagatcaa cataatcagt acgcaatttt tgcaggctct
     3001 ctttcagact cgggatcaat ttgtctttgc tgagattttc aatccagatt ttagtggtga
     3061 tgtagagttc atgacgtggc acgccacttt ctgcaatcgc ctgacctact gcggcttcgt
     3121 tatcatagat ttgtgcagta tcaattgcgc gataaccaag ttcaagcgcc gttttcacag
     3181 atgaaataac aacgtcgtct ttcagacgga aagtacctaa accaaatgca gggatagcca
     3241 taatatgcct cttttaatgc tattttcgtt aactgaggtg attatgggct tcagactgtt
     3301 gaggaaaaag agcgtaaaat gcagagaatt tttgcgattc tggcaataat agatatacag
     3361 acgaaaaaaa acctcgagcg ttaactcgag gcttcttaat aagtggcgga acggacggga
     3421 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
     3481 accgaattct tttacaacca ccggttttat gaccggctta ctgcttaatt tgatgcctgg
     3541 cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact
     3601 tctgagttcg gcatggggtc aggtgggacc accgcgctac ggccgccagg caaattcctg
     3661 tgctctgtcc tgtgtctttt gtgctgatac tgcgtcggct gcattcgcga aagtcagtca
     3721 catacttcag tatgctcctt ccttcgcttc atttgccgcc ttgtctcagc gcaaaatcct
     3781 tcggactctg aatcaaagct gaaattctct ca
//
LOCUS       DACAPB010000289         3800 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.294, whole genome shotgun sequence.
ACCESSION   DACAPB010000289 DACAPB010000000
VERSION     DACAPB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.294"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..904
                     /gene="sufD"
                     /locus_tag="IT904_004543"
     CDS             <1..904
                     /gene="sufD"
                     /locus_tag="IT904_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP3194216.1"
                     /translation="EVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGVAGEEVN
                     TAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKLAFENPVS
                     HHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCD
                     TRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLA
                     EVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYAFAAELTE
                     ALRDEGLKQQVLARIGQRLPGGVR"
     gene            901..2121
                     /gene="sufS"
                     /locus_tag="IT904_004544"
     CDS             901..2121
                     /gene="sufS"
                     /locus_tag="IT904_004544"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAP3194217.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPGQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKQASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHLVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            2134..2550
                     /gene="sufE"
                     /locus_tag="IT904_004545"
     CDS             2134..2550
                     /gene="sufE"
                     /locus_tag="IT904_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAP3194218.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            2699..3703
                     /gene="ldtE"
                     /locus_tag="IT904_004546"
     CDS             2699..3703
                     /gene="ldtE"
                     /locus_tag="IT904_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAP3194219.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRVTQ
                     "
BASE COUNT          939 a          946 c         1049 g          866 t
ORIGIN      
        1 ggaagtgttt ctgcatttga cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa
       61 gcgcggtcaa cggccggcaa agccattgct gttaatgcat attacccagg gcgtggcagg
      121 tgaagaggtc aacactgccc attaccgaca tcatctggat ctggcggaag gtgccgaagc
      181 aacggtgatc gaacattttg tcagccttaa tgatgctcgt cacttcaccg gcgcacggtt
      241 cactatcaat gtcgcagcga acgcccactt gcagcatatc aagctggcgt ttgaaaaccc
      301 ggtcagtcac cactttgccc ataacgattt gttgctggct gacgatgcca ccgcatttag
      361 ccatagtttc ctgctgggtg gcgcagtgtt acgacacaac accagtacgc aactcaatgg
      421 cgaaaacagc acgctgcgga tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga
      481 tacccgcacc tggctggaac acaataaagg tttttgtaac agccgacagt tgcacaaaac
      541 tatcgtcagc gacaaaggcc gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc
      601 catcaaaacg gatggtcaga tgaccaacaa taatctgctg atgggcaaac tggcggaagt
      661 ggatacgaaa ccgcagctgg aaatctatgc agatgatgtg aaatgcagcc acggcgcgac
      721 ggtggggcgt attgatgatg aacagatgtt ctatctgcgc tcgcgcggga tcaatcagca
      781 ggatgcccag cagatgatca tttacgcctt tgctgctgaa ctgacggaag cactgcgtga
      841 tgaggggctt aaacagcagg tgctggcccg aatcggtcaa cgactgccag gaggtgtaag
      901 atgacttttt ccgtcgacaa agtgcgggcc gactttccgg tgctttctcg tgaggtaaac
      961 ggtttgccgc tggcttatct cgacagcgcc gccagtgcgc agaaaccggg ccaggtgatt
     1021 gacgccgagg ccgagtttta tcgtcacggc tacgcggcgg tgcatcgcgg tattcatacc
     1081 ttaagcgccc aggcgaccga gaaaatggag aacgtacgca agcaggcatc gttgtttatc
     1141 aacgcccgtt cggcggaaga gctggtgttc gtccgcggca cgacggaagg gatcaatctg
     1201 gtcgccaata gctggggcaa cagcaacgtg cgggcgggcg ataacatcat cataagtcag
     1261 atggagcacc acgctaacat tgttccctgg cagatgcttt gcgcacgcgt tggcgcagag
     1321 ctgcgtgtga tcccgctcaa tcccgacggt acgttgcaac tggagacgct gcctacgctg
     1381 tttgatgaga aaactcgcct gctggcaatt actcatgtct ccaacgtgct tggcacagaa
     1441 aatccactgg cggaaatgat cacgcttgcg caccagcatg gcgcaaaagt gctggtggat
     1501 ggcgctcagg cggtgatgca tcatctggtg gatgttcagg cgctggattg cgacttttac
     1561 gtgttctccg ggcataaact gtatggcccc accggaattg gcattcttta tgtcaaagaa
     1621 gccttgttgc aggagatgcc gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc
     1681 ctgagtgaag gcactacctg gaccaaagca ccatggcggt ttgaagccgg tacacccaat
     1741 accgggggca tcattggtct tggcgcggcg ctggagtatg tttcggcgct ggggcttaat
     1801 aacatagccg agtatgaaca gaatctgatg cattatgcgc tatcacagct ggaatctgta
     1861 ccggatctca ctctctatgg cccacaaaac aggcttggcg ttattgcttt taatctcggt
     1921 aaacaccacg cctatgatgt tggcagtttt ctcgataatt acggcattgc tgtgcgtacc
     1981 ggacatcact gcgcaatgcc attgatggcc tattacaacg tccctgcgat gtgtcgggcg
     2041 tcgctggcca tgtataacac ccatgaagaa gtggatcgtc tggtgaccgg cctgcaacgt
     2101 attcaccgtt tgctgggata acagggaggc actatggctt tattgccgga taaagaaaag
     2161 ttgctgcgta attttttacg ctgcgccaac tgggaagaga aatatctcta cattattgag
     2221 ctgggccagc gtctgccaga attacgcgac gaagacagaa gtccacaaaa tagcattcag
     2281 ggctgtcaga gtcaggtgtg gattgtcatg cgccagaatg cccagggaat tattgaatta
     2341 cagggcgaca gcgatgcggc gattgtgaaa gggcttattg cggtcgtctt tattctctac
     2401 gatcagatga cgccgcagga tattgtcaat ttcgatgtgc gtccgtggtt tgaaaaaatg
     2461 gcgctcaccc aacatctcac cccatctcgt tcacaaggtc tggaagcgat gattcgcgca
     2521 attcgcgcca aagccgctgc acttagctaa actaaaagga cagctttcat ccggttggct
     2581 cacattgccg gatggtcaat gtttacactg acctgcacag gagtgttttg gtttcaggtg
     2641 aacataagcg ccatccgacg tttcagcgtg agtctccggc aaatacagga ggtttacaat
     2701 gaaacgcgcg tctttgctta cactcacgct tatcggcgct tttagcgcca tccaggctgc
     2761 ctgggcggtt gattatccgc taccaccaac cggaagccga ctggttgggc aaaatcaaac
     2821 gtatacggtg caagaagggg ataaaaacct tcaggctatt gcccgacgtt ttgatactgc
     2881 ggcaatgttg atccttgaag ccaataacac tatcgccccg gtgccaaaac ctggtacgac
     2941 gataactatt ccttcacaac tgttattacc tgatgcaccg cgtcagggga ttatcgttaa
     3001 ccttgcagag ctgcgccttt attattatcc gccgggagaa aatattgtgc aggtttatcc
     3061 aataggtatt ggattgcagg ggctggaaac gccggtgatg gaaacgcgtg ttgggcagaa
     3121 aatccctaac ccaacctgga cgcctacggc aggcattcgt cagcgttcgc tggagcgtgg
     3181 cattaaatta ccgccagtcg ttcctgccgg accaaataac ccgctaggac gttacgcact
     3241 gcgcctcgcg catggtaatg gcgaatacct cattcatggt accagtgcgc cggacagcgt
     3301 cggtttgcgc gtcagttcag ggtgtattcg catgaatgca ccggatatta aagccttgtt
     3361 ctccagcgtg cggacgggaa cgccggtgaa agtgatcaac gaaccggtga aatattccgt
     3421 ggagcctaac gggatgcgtt atgttgaagt acatcgacca ctatcggcag aagaacagca
     3481 gaacgttcag acaatgccat acacactgcc agcaggcttt acgcaattta aagacaataa
     3541 ggctgtagat cagaagttag tcgataaagc gttgtatcgt cgggcagggt atccggtttc
     3601 ggtgagcagt ggagcaactc ccgcagccag caatgcgcct tcagtagagt cagcgcagaa
     3661 tggtgaacca gagcaaggga atatgttacg cgtgacgcag tagcggtaaa cggcagacaa
     3721 aaaaaatggc gcacaatgtg cgccattttt cacttcacag gtactattac ttgcggtatt
     3781 tagtagccat gttgtccaga
//
LOCUS       DACAPB010000290         3754 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.295, whole genome shotgun sequence.
ACCESSION   DACAPB010000290 DACAPB010000000
VERSION     DACAPB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.295"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            37..480
                     /locus_tag="IT904_004547"
     CDS             37..480
                     /locus_tag="IT904_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAP3194220.1"
                     /translation="MVAASLGLPALSNAAEPNAPAKATTRNHEPSAKVNFGQLALLEA
                     NTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTLPVAEESLPLQAQHLALLDTLSAL
                     LTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQGIRAGPQRLT"
     gene            542..3247
                     /gene="secA"
                     /locus_tag="IT904_004548"
     CDS             542..3247
                     /gene="secA"
                     /locus_tag="IT904_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP3194221.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            3307..3696
                     /gene="mutT"
                     /locus_tag="IT904_004549"
     CDS             3307..3696
                     /gene="mutT"
                     /locus_tag="IT904_004549"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAP3194222.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
BASE COUNT          996 a          936 c         1010 g          812 t
ORIGIN      
        1 ggtaaacgct acttctggcc gcatctctta ttagggatgg ttgcggcgag tttaggtttg
       61 cctgcgctca gcaacgccgc cgaaccaaac gcgcccgcaa aagcgacaac ccgcaaccac
      121 gagccttcag ccaaagttaa ctttggtcaa ttggccttgc tggaagcgaa cacacgccgc
      181 ccgaattcga actattccgt tgattactgg catcaacatg ccattcgcac ggtaatccgt
      241 catctttctt tcgcaatggc accgcaaaca ctgcccgttg ctgaagaatc tttgcctctt
      301 caggcgcaac atcttgcatt actggatacg ctcagcgcgc tgctgaccca ggaaggcacg
      361 ccgtctgaaa agggttatcg cattgattat gcgcatttta ccccacaagc aaaattcagc
      421 acgcccgtct ggataagcca ggcgcaaggc atccgtgctg gccctcaacg cctcacctaa
      481 caacaataaa cctttacttc attttattaa ctccgcaacg cggggcgttt gagattttat
      541 tatgctaatc aaattgttaa ctaaagtttt cggtagtcgt aacgatcgca ccctgcgccg
      601 gatgcgcaaa gtggtcaaca tcatcaatgc catggaaccg gagatggaaa aactctccga
      661 cgaagaactg aaagggaaaa ccgcagagtt tcgtgcacgt ctggaaaaag gcgaagtgct
      721 ggaaaatctg atcccggaag ctttcgccgt ggtacgtgag gcaagtaagc gcgtctttgg
      781 tatgcgtcac ttcgacgttc agttactcgg cggtatggtt cttaacgaac gctgcatcgc
      841 cgaaatgcgt accggtgaag gaaaaaccct gaccgcaacg ctgcctgctt acctgaacgc
      901 actaaccggt aaaggcgtgc acgtagttac cgtcaacgac tacctggcgc aacgtgacgc
      961 cgaaaacaac cgtccgctgt ttgaattcct tggcctgact gtcggtatca acctgccggg
     1021 catgccagca ccggcaaagc gcgaagctta cgcagctgac atcacttacg gtacgaacaa
     1081 cgaatacggc tttgactacc tgcgcgacaa catggcgttc agccctgaag aacgtgtaca
     1141 gcgtaaactg cactatgcgc tggtggacga agtggactcc atcctgatcg atgaagcgcg
     1201 tacaccgctg atcatttccg gcccggcaga agacagctcg gaaatgtata aacgcgtgaa
     1261 taaaattatt ccgcacctga tccgtcagga aaaagaagac tccgaaacct tccagggcga
     1321 aggccacttc tcggtggacg aaaaatctcg ccaggtgaac ctgaccgaac gtggtctggt
     1381 gctgattgaa gaactgctgg tgaaagaggg catcatggat gaaggggagt ctctgtactc
     1441 tccggccaac atcatgctga tgcaccacgt aacggcggcg ctgcgcgctc atgcgctgtt
     1501 tacccgtgac gtcgactaca tcgttaaaga tggtgaagtt atcatcgttg acgaacacac
     1561 cggtcgtacc atgcagggcc gtcgctggtc cgatggtctg caccaggctg tggaagcgaa
     1621 agaaggtgtg cagatccaga acgaaaacca aacgctggct tcgatcacct tccagaacta
     1681 cttccgtctg tatgaaaaac tggcggggat gaccggtact gctgataccg aagctttcga
     1741 atttagctca atctacaagc tggataccgt cgttgttccg accaaccgtc caatgattcg
     1801 taaagatctg ccggacctgg tctacatgac tgaagcggaa aaaattcagg cgatcattga
     1861 agatatcaaa gaacgtactg cgaaaggcca gccggtgctg gtgggtacta tctccatcga
     1921 aaaatcggag ctggtgtcaa acgaactgac caaagccggt attaagcaca acgtcctgaa
     1981 cgccaaattc cacgccaacg aagcggcgat tgttgctcag gcaggttatc cggctgcggt
     2041 gactatcgcg accaatatgg cgggtcgtgg tacagatatt gtgctcggtg gtagctggca
     2101 ggcagaagtt gccgcgctgg aaaatccgac cgcagagcaa attgaaaaaa ttaaagccga
     2161 ctggcaggta cgtcacgatg cggtactggc agcaggtggc ctgcatatca tcggtaccga
     2221 gcgtcacgaa tcccgtcgta tcgataacca gttgcgcggt cgttctggtc gtcaggggga
     2281 tgctggttct tcccgtttct acctgtcgat ggaagatgcg ctgatgcgta tttttgcttc
     2341 cgaccgagta tccggcatga tgcgtaaact gggtatgaag ccaggcgaag ccattgaaca
     2401 cccgtgggtg accaaagcga ttgccaacgc ccagcgtaaa gttgaaagtc gtaacttcga
     2461 cattcgtaag caactgctgg aatatgatga cgtggctaac gatcagcgtc gcgccattta
     2521 ctcccagcgt aacgaactgc tggatgtcag cgatgtgagc gaaaccatca acagcattcg
     2581 tgaagatgtg ttcaaagcca ccattgatgc ctacattccg ccgcagtcgc tggaagaaat
     2641 gtgggatatt ccggggctgc aggaacgtct gaagaacgat ttcgacctcg atttaccaat
     2701 tgctgagtgg ctggataaag aacccgaact gcatgaagag acgctgcgtg agcgcattct
     2761 ggcgcagtcc atcgaagtgt atcagcgtaa agaagaagtg gttggtgcag agatgatgcg
     2821 tcacttcgag aaaggcgtca tgctgcaaac gcttgactcc ctgtggaaag agcacctggc
     2881 agcgatggac tatctgcgtc agggtatcca cctgcgaggc tatgcacaga aagatccgaa
     2941 gcaggaatac aaacgtgaat cgttctccat gtttgcagcg atgctggagt cgttgaaata
     3001 tgaagttatc agtacgctga gcaaagttca ggtacgtatg cctgaagagg ttgaggagct
     3061 ggaacaacag cgtcgtatgg aagccgagcg tttagcgcaa atgcagcagc ttagccatca
     3121 ggatgacgac tctgcagccg cagctgcact ggcggcgcaa accggtgaac gcaaagtagg
     3181 acgtaacgat ccttgcccgt gcggttctgg taaaaaatac aagcagtgcc atggtcgcct
     3241 gcaataaaag ctaacagttg aagtaaaagg cgcaggattc tgcgcctttt ttataggttt
     3301 aagacaatga aaaagctgca aattgcggta ggtattattc gcaacgagaa caatgaaatc
     3361 tttataacgc gtcgcgcagc agatgcgcac atggcgaata aactggagtt tcccggcggt
     3421 aaaattgaaa tgggtgaaac gccggaacag gcggtggtgc gtgaacttca ggaagaagtc
     3481 gggattaccc cacaacattt ttcgctattt gaaaaactgg aatatgaatt cccggacagg
     3541 catataacac tgtggttctg gctggtcgaa cgctgggaag ggaagccgtg gggtaaagaa
     3601 gggcaacccg gtgagtggat ggcgctggtc gatcttaatg ccgatgattt tccgccagcc
     3661 aatgaaccgg taattgcgaa gcttaaacgt ctgtaggtca gataaggcgt tttcgccgca
     3721 tccgacattc gcacaagatg cctgatgcga cgct
//
LOCUS       DACAPB010000291         3692 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.296, whole genome shotgun sequence.
ACCESSION   DACAPB010000291 DACAPB010000000
VERSION     DACAPB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.296"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..495
                     /locus_tag="IT904_004550"
     rRNA            <1..495
                     /locus_tag="IT904_004550"
                     /product="16S ribosomal RNA"
     gene            552..628
                     /locus_tag="IT904_004551"
     tRNA            552..628
                     /locus_tag="IT904_004551"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:586..588,aa:Ile,seq:gat)
     gene            671..746
                     /locus_tag="IT904_004552"
     tRNA            671..746
                     /locus_tag="IT904_004552"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Ala,seq:tgc)
     gene            912..>3692
                     /locus_tag="IT904_004553"
     rRNA            912..>3692
                     /locus_tag="IT904_004553"
                     /product="23S ribosomal RNA"
BASE COUNT          975 a          819 c         1118 g          780 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgtact ttgcagtgct cacacagatt gtctgatgaa aatgagcagt
      541 aaaacctcta caggcttgta gctcaggtgg ttagagcgca cccctgataa gggtgaggtc
      601 ggtggttcaa gtccactcag gcctaccaaa tttgcacggc aaatttgaag aggttttaac
      661 tacatgttat ggggctatag ctcagctggg agagcgcctg ctttgcacgc aggaggtctg
      721 cggttcgatc ccgcatagct ccaccatctc tgtagtgatt aaataaaaaa tacttcagag
      781 tgtacctgca aaggttcact gcgaagtttt gctctttaaa aatctggatc aagctgaaaa
      841 ttgaaacact gaacaacgaa agttgttcgt gagtctctca aattttcgca actctgaagt
      901 gaaacatctt cgggttgtga ggttaagcga ctaagcgtac acggtggatg ccctggcagt
      961 cagaggcgat gaaggacgtg ctaatctgcg ataagcgtcg gtaaggtgat atgaaccgtt
     1021 ataaccggcg atttccgaat ggggaaaccc agtgtgtttc gacacactat cattaactga
     1081 atccataggt taatgaggcg aaccggggga actgaaacat ctaagtaccc cgaggaaaag
     1141 aaatcaaccg agattccccc agtagcggcg agcgaacggg gagcagccca gagcctgaat
     1201 cagtgtgtgt gttagtggaa gcgtctggaa aggcgcgcga tacagggtga cagccccgta
     1261 cacaaaaatg cacatgctgt gagctcgatg agtagggcgg gacacgtggt atcctgtctg
     1321 aatatggggg gaccatcctc caaggctaaa tactcctgac tgaccgatag tgaaccagta
     1381 ccgtgaggga aaggcgaaaa gaaccccggc gaggggagtg aaaaagaacc tgaaaccgtg
     1441 tacgtacaag cagtgggagc acgcttaggc gtgtgactgc gtaccttttg tataatgggt
     1501 cagcgactta tattctgtag caaggttaac cgaatagggg agccgaaggg aaaccgagtc
     1561 ttaactgggc gttaagttgc agggtataga cccgaaaccc ggtgatctag ccatgggcag
     1621 gttgaaggtt gggtaacact aactggagga ccgaaccgac taatgttgaa aaattagcgg
     1681 atgacttgtg gctgggggtg aaaggccaat caaaccggga gatagctggt tctccccgaa
     1741 agctatttag gtagcgcctc gtgaattcat ctccgggggt agagcactgt ttcggcaagg
     1801 gggtcatccc gacttaccaa cccgatgcaa actgcgaata ccggagaatg ttatcacggg
     1861 agacacacgg cgggtgctaa cgtccgtcgt gaagagggaa acaacccaga ccgccagcta
     1921 aggtcccaaa gtcatggtta agtgggaaac gatgtgggaa ggcccagaca gccaggatgt
     1981 tggcttagaa gcagccatca tttaaagaaa gcgtaatagc tcactggtcg agtcggcctg
     2041 cgcggaagat gtaacggggc taaaccatgc accgaagctg cggcagcgac gcttatgcgt
     2101 tgttgggtag gggagcgttc tgtaagcctg tgaaggtgtg ctgtgaggca tgctggaggt
     2161 atcagaagtg cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg
     2221 aagaccaagg gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag
     2281 gccgaaaggc gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa
     2341 ggggggacgg agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc
     2401 tggttttcca ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg
     2461 ctgaagcaac aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc
     2521 gtaccccaaa ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg
     2581 gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt
     2641 gaagtccctc gcggatggag ctgaaatcag tcgaagatac cagctggctg caactgttta
     2701 ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg
     2761 tgccggaagg ttaattgatg gggtcagcgc aagcgaagct cttgatcgaa gccccggtaa
     2821 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
     2881 cctgcacgaa tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa
     2941 ctcgctgtga agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta
     3001 tagcttgaca ctgaacattg agccttgatg tgtaggatag gtgggaggct tagaagtgtg
     3061 gacgccagtc tgcatggagc cgaccttgaa ataccaccct ttaatgtttg atgttctaac
     3121 gttgacccgt aatccgggtt gcggacagtg tctggtgggt agtttgactg gggcggtctc
     3181 ctcctaaaga gtaacggagg agcacgaagg ttggctaatc ctggtcggac atcaggaggt
     3241 tagtgcaatg gcataagcca gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc
     3301 aggtcatagt gatccggtgg ttctgaatgg aagggccatc gctcaacgga taaaaggtac
     3361 tccggggata acaggctgat accgcccaag agttcatatc gacggcggtg tttggcacct
     3421 cgatgtcggc tcatcacatc ctggggctga agtaggtccc aagggtatgg ctgttcgcca
     3481 tttaaagtgg tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc
     3541 gtgggcgctg gagaactgag gggggctgct cctagtacga gaggaccgga gtggacgcat
     3601 cactggtgtt cgggttgtca tgccaatggc actgcccggt agctaaatgc ggaagagata
     3661 agtgctgaaa gcatctaagc acgaaacttg cc
//
LOCUS       DACAPB010000292         3633 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.298, whole genome shotgun sequence.
ACCESSION   DACAPB010000292 DACAPB010000000
VERSION     DACAPB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.298"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            54..1625
                     /gene="bcsE"
                     /locus_tag="IT904_004554"
     CDS             54..1625
                     /gene="bcsE"
                     /locus_tag="IT904_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAP3194223.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            1622..1813
                     /gene="bcsF"
                     /locus_tag="IT904_004555"
     CDS             1622..1813
                     /gene="bcsF"
                     /locus_tag="IT904_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAP3194224.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKTRATKK"
     gene            1810..3489
                     /gene="bcsG"
                     /locus_tag="IT904_004556"
     CDS             1810..3489
                     /gene="bcsG"
                     /locus_tag="IT904_004556"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAP3194225.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPLPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGLDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIEQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3576..>3633)
                     /gene="ldrD"
                     /locus_tag="IT904_004557"
     CDS             complement(3576..>3633)
                     /gene="ldrD"
                     /locus_tag="IT904_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAP3194226.1"
                     /translation="VIAGILASMIVNWLNKRK"
BASE COUNT          861 a          932 c          913 g          927 t
ORIGIN      
        1 aggtttattt cttagctttc gctagtaaac tgataatcag acaaaatggt gacatgaggg
       61 acattgtgga ccctgtattc tctatcggta tctcatcatt atgggatgag ctgcgacata
      121 tgccagcagg cggcgtctgg tggtttaacg tcgatcgcca tgaagatgct atcagtctgg
      181 cgaatcaaac aattgcatcc caggctgaaa ccgcacacgt cgcggtcatt agcatggaca
      241 gcgatccggc gaaaatcttt caattagatg attctcaagg gccggaaaaa ataaaattat
      301 tttcaatgct aaatcatgaa aaaggtctat actatttgac ccgtgatttg cagtgttcta
      361 ttgatcccca taattacctt tttattcttg tttgcgcaaa taacgcatgg caaaacattc
      421 ctgccgagcg gcttcgctca tggttggata aaatgaataa atggagcagg ttaaaccatt
      481 gttcgctttt ggtaattaat cccggaaata ataacgataa acaattttca ttgttgcttg
      541 aggaataccg ttcacttttt ggtcttgcca gtttgcgttt tcagggtgac caacatttgc
      601 tggatattgc cttctggtgc aacgaaaaag gggtcagcgc ccgtcagcag cttagcgttc
      661 agcaacaaaa tggtatctgg acattagttc aaagcgaaga ggcggagatc caaccacgca
      721 gcgacgaaaa acgcattctg agtaatgttg ctgtactgga aggtgcgccg ccgctatcgg
      781 aacactggca actgttcaac aataacgaag tcctgttcaa tgaagcccgt accgctcagg
      841 cggcgacggt ggtcttttct ttacagcaaa atgcgcaaat cgagccactg gcccgcagca
      901 ttcataccct gcgtcgccag cgcggtagtg cgatgaaaat cctcgtgcgg gaaaataccg
      961 ctagcctgcg cgccaccgat gaacgtttgt tattggcctg cggtgcaaat atggttattc
     1021 cgtggaatgc gccactctcc cgttgtctga cgatgatcga aagcgtgcaa gggcagaagt
     1081 ttagtcgcta tgtgccggaa gatatcacta ccttgctgtc aatgacccag ccgctcaaac
     1141 tgcgtggttt ccagaagtgg gatgtgttct gtaatgccgt caacaacatg atgaataacc
     1201 ctctattacc tgcccacggt aaaggcgttc tggttgccct acgtccggta ccgggtatcc
     1261 gcgttgaaca agccctgacg ctgtgtcgcc ctaaccgtac cggcgatatc atgaccattg
     1321 gcggtaatcg gctggtgctg tttctctcat tctgtcggat taacgatctg gataccgcgt
     1381 tgaatcatat tttcccattg cctactggcg acattttctc aaaccgtatg gtctggtttg
     1441 aagatgatca aatcagtgcc gagctggtgc agatgcgctt gcttgcccca gaacaatggg
     1501 gcatgccgct gcctttaacg caaagttcta aaccggtcat caatgccgag cacgatggtc
     1561 gccactggcg acgaatacca gaacccatgc gactgttaga tgatgctgtg gagcgctcat
     1621 catgatgacc atcagcgata tcattgaaat tattgtcgtt tgcgcactga tatttttccc
     1681 gctgggctat ctggcgcggc actctttgcg acgcattcgc gacaccttac gtttgttctt
     1741 tgctaaacct cgttatgtta aaccggccgg gacgttacgc cgcacggaaa aaaccagggc
     1801 aaccaaaaaa tgactcaatt tacgcaaaat accgccatgc cttcttccct ctggcaatac
     1861 tggcgcggcc tttccggctg gaacttctat tttctggtta agttcggcct gttgtgggcg
     1921 ggatatctta acttccatcc gctcctcaat ttggtgtttg ccgcgtttct gctgatgccc
     1981 cttccgcgct acagcctgca tcgcttgcgc cactggattg ccctgccgat cggctttgct
     2041 ttgttctggc atgacacctg gttgcctggc ccggaaagca taatgagcca gggttcgcag
     2101 gtggcggggt tcagtaccga ttatttaatc gaccttgtca cacgctttat taactggcag
     2161 atgattgggg ccatttttgt tttattagtg gcctggttat tcctgtcaca atggattcgc
     2221 attaccgttt ttgtggttgc catactgcta tggctgaacg tacttaccct ggcgggacca
     2281 agtttctcct tgtggccagc cggacaaccg acgaccactg taacaacgac gggtggtaac
     2341 gcagcggcaa ccgttgcggc gacgggtggc gcaccggtag tgggtgatat gcccgcacaa
     2401 actgcaccgc caacaacggc gaaccttaac gcctggctga ataatttcta taacgcggag
     2461 gcgaaacgta aatcgacctt cccgtcttcg ctgcccgctg atgctcagcc atttgaacta
     2521 ctggtgatta acatctgttc gctttcctgg tcggatatag aagccgccgg gttgatgtcg
     2581 catccactgt ggtcgcattt cgatattgag ttcaagaact ttaactccgc cacctcctac
     2641 agtggcccgg cggcgatccg tttactgcgc gccagctgcg ggcagacttc gcacactaat
     2701 ctgtatcaac cggcaaataa cgactgctat ctgtttgata acctttcgaa actgggcttt
     2761 acccagcacc tgatgatggg acataacggc cagttcggcg gttttttgaa agaagttcgc
     2821 gaaaatggcg gcatgcagag cgaattgatg gatcaaacaa atctgccggt tattttgctg
     2881 ggccttgatg gttcgccggt ttatgacgat accgctgtgc ttaaccgctg gctggacgtt
     2941 accgaaaaag ataaaaacag ccgtagtgcc acgttctaca acacgcttcc actgcatgac
     3001 ggcaaccatt atccgggggt cagcaaaaca gcggattaca aagcgcgggc gcagaaattc
     3061 tttgatgaac tggacgcctt ctttactgaa cttgagaaat cgggtcgtaa agtgatggtg
     3121 gtcgtggtgc cggaacacgg cggcgcgctg aagggcgaca gaatgcaggt atctggccta
     3181 cgtgatatcc ctagcccgtc tatcaccgac gtccccgttg gggtgaaatt cttcggcatg
     3241 aaggcaccgc atcagggggc accgattgtc atcgaacaac cgagcagctt cctggctatc
     3301 tccgatctgg tggttcgcgt tctcgatggc aagattttca ccgaagacaa tgttgactgg
     3361 aaaaaactca ccagtgggtt gccacaaaca gcaccggtct ccgagaactc aaatgcagta
     3421 gttattcaat accaggataa accgtacgtt cgcctgaacg gcggcgactg ggtgccttac
     3481 ccgcagtaag aacgaaaaag gccgcagagt ttcccccctg cggcctggtc cgggcgcaaa
     3541 ttgccattac ggcagcctga cgcccgcatg acacgttact tccgcttgtt cagccagttc
     3601 acgatcatac tggcaagaat gccagcaatg acc
//
LOCUS       DACAPB010000293         3486 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.299, whole genome shotgun sequence.
ACCESSION   DACAPB010000293 DACAPB010000000
VERSION     DACAPB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.299"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..689)
                     /locus_tag="IT904_004558"
     CDS             complement(<1..689)
                     /locus_tag="IT904_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP3194227.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGTLPTP
                     DAKQTALVNEKRRAALNMLYIDPHNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLSTRKYVDDKISEHE
                     QSRRHPDASLTAKGFTQLSSATNSESEILAATPKAVKAAYDLAAGKASASHTHPWSQI
                     TDVPAASLTVKG"
     gene            complement(686..1291)
                     /locus_tag="IT904_004559"
     CDS             complement(686..1291)
                     /locus_tag="IT904_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP3194228.1"
                     /translation="MNSLMPPGSTSLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGEAPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(1284..2192)
                     /locus_tag="IT904_004560"
     CDS             complement(1284..2192)
                     /locus_tag="IT904_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP3194229.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAIGSDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDLVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKVSLQRYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(2179..2538)
                     /locus_tag="IT904_004561"
     CDS             complement(2179..2538)
                     /locus_tag="IT904_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP3194230.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITIKSHFDGSMVVELSGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(2535..3113)
                     /locus_tag="IT904_004562"
     CDS             complement(2535..3113)
                     /locus_tag="IT904_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP3194231.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGELETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAIYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITLKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(3182..>3486)
                     /locus_tag="IT904_004563"
     CDS             complement(3182..>3486)
                     /locus_tag="IT904_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP3194232.1"
                     /translation="DGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASADSASV
                     QFESKVQRIARVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
BASE COUNT          721 a         1055 c          899 g          811 t
ORIGIN      
        1 ccttttaccg tcagtgaagc tgcaggcaca tccgttatct ggctccacgg gtgtgtgtga
       61 ctggcggatg ctttacctgc tgcaagatca tatgcagcct tcacagcctt cggtgttgcg
      121 gccagtattt cggattcact gttggtcgca ctgcttaact gagtaaaacc ttttgcggtc
      181 agcgaggcgt ccgggtgacg tcgtgactgt tcgtgctctg atattttgtc atccacatac
      241 ttgcgggttg acagcactac ggcagggtca attttcaggg tgatattgtc cgtactgctg
      301 gtaatcagca ccatgcgcac ggtctgggtg cgcccgctgc cttcagccag ttgcggctta
      361 tagctttccg ggcagttgcc tacggcaatc aatgccccgg actcatcaaa cagacccact
      421 tcacgtatcc accaaccacc ctcattttca gggatcacct gttcagcaat aatctggctg
      481 ctgttatgcg ggtcgatata gagcatattc agcgcagccc ggcgtttctc atttactaat
      541 gctgtctgct ttgcgtccgg cgttggcaat gttccgccgc catcgccgac cgccatatgg
      601 gtaattttta aaggcacacc gagcgcggcg gcgctggcaa gtttcgccgc gccaatatcc
      661 gtcagcaggg tataaaattt tgtgctcatg gattcactct cattgtgtca ataacatgga
      721 ccgccccgcc ttcatgcgcg gtgccaccgg aaataatcgt ttcgttgata tacggataga
      781 tcgtgatttc ttcgccaaga tagctggcgg ctcccaccca atgcgggccg ctggtctgca
      841 gattgatgga catgccgatc atgtggcggc tacatggttt ggcatcgctt atcagtcgct
      901 caagttccag ataggtatct tcagtgatgc cctgatcctg cacgccgata tccaggcgaa
      961 acgtgcccgg tgcctctccg gtctgccacc actcaataat gcggatcaga aagccgaacg
     1021 gctccaccac ccgccgcacg gcactggtgg tccctttatg ctgatgaata taaaaagcat
     1081 ccttcaccac ctggcgcttg acgctttctg tccagccctc gtcccagcga tccacagaga
     1141 acgcccaggc gagataaggc aggaaactga ccggacaggt tgccggattc cacaagtcac
     1201 gcagcggcac ctgcagatca gaaatcccgc tgcaggtttg cgccagtcgt cgctccagtg
     1261 aagttgaacc cggtggcatc agactattca tccgttcctc cgttggtcac gctccactgc
     1321 gtacatgatg ccgcctgcgt tttgttcaga accacatccg ccagcggaga agccagttcc
     1381 acacgttgaa caccctcaac atgcaacgcg gcaaaaatag cactacggcg aatatcccga
     1441 ccgagccgcg tctgactggc gatgtacctc tgcagactga cttttgccgc tgccattacc
     1501 ggctctgctt ccggccccgg gtagagaaaa atggtggcct ccacgcggta cgggatgatt
     1561 tctgcgctgc gaaccgtcag acggtcagcc accgggcgga cgttctcact gttcagggct
     1621 ttctccacca aatccagcag gtctttttct gctgttccgt ccccttcgcg gctaaggaca
     1681 gtcagcacca cctctgcagg tgccgggctg gttgcactgg catccgccac ccgaccgtcg
     1741 gcgcttcgtg catgaaactc ataagctgca gttggccccg caactgaaag cccttcaaag
     1801 gcggcaggca cacgcaggcg taacgcttca tcactttcca tcacagccgc aacgggcggc
     1861 acagcgtcat tatcagcagg tgtcaccgtc aggcgtttca cgttgtaatt ggcagcaagc
     1921 tggtccagat cgctccctat ggcataagcc accatcaccg cctgcgcggc ttcgttaatg
     1981 cgctgacgca gaagcaactc acgataagcg ttctcctgca acaatttggt aacgggttca
     2041 gattccagtt ccagcgtgcg gatcactgct tcctgctcat ctttcggatg aagcgccaca
     2101 aattctgcct tgcgttcggc aagcagcgtc tcaaagtccg gcacatccac aatctgcggc
     2161 gcaggcaact gcgaaaggtc aatcactgcc attctctgct cctgttgata cggaaaggga
     2221 aacaggcaca ccgttattac gccgcccgct cagctccacc accattgaac cgtcaaaatg
     2281 gcttttgatg gtgatggaat ccagcgtcag ccgtggctcc cagcgactca gcgccacata
     2341 cactgccgac atgacctgca ggcgtaacgc cggattttgt ggctggtcta tcagtgctga
     2401 cagcagggaa ccatattcac ggcgagcaat gcggctaccc tgcggcgtca gcagaatgtc
     2461 ccgcaccgac tggcgcagat ggtcaatatc agtaatggct ttgccgctgg tattgttcat
     2521 cccgctataa agcgtcatac cgggcctccg gttgtgtcgc cgcctttcag aacgccagta
     2581 tgctgatgcg catcaaccac gatcccgtta gaactcatcg ctccgccgcc ctgggtaacg
     2641 ccaccattga tcaccacttc gctgttaata cgcgtgcggt cagcctccag cacaaactca
     2701 ctggttttca gggtgatatt gtcggcggcc tcaatgacca ttgatttgat gcccctgaca
     2761 taccagcgcc cagtggctgg ttcgtattca aaccagccac cgtcaggatg ttctgtcacg
     2821 caggcgtccg ccgacgtcga cggtggcgcg aactgattcg aatagatggc gggtaacgca
     2881 aaggcggttt ccagattgcc gcccagactc agcagcacca cctgctcacc ttctgatggt
     2941 cgccaccatg tgcgggcatt cccggcacgc agcgtcagcc agctgatcca gttggtttca
     3001 agctcgcccg ttttcacccg gcaaagccag ttttccctgt ccacttcggt gactacccct
     3061 gtgcggatca ggttggtgat aaggcgcatg atttcggtta attgtgcgtt catagggaaa
     3121 ggttgccatc aggggaagaa aggcggcagt gctgcaactt gtatcagtgc tgatacaaag
     3181 atcaccccgc cagccattgc agaatcatgt cgcgagtcat tgcctcaaca tcatcattta
     3241 cacccagcag gcggcgctct gcgtaacgga cctccggtcc tttgcgactg acgcgatcac
     3301 gcaggccgta atggtgaaca cgggcaatac gctgcacctt gctttcaaac tgtacgctgg
     3361 cagagtcggc gctggcggca gttttcaggt attttgtggt gcgcagcttt gcaaacatct
     3421 gacgtttgat gcgccccttc ttgctgcgtg ctgttaccct gcgcggttca taactgctgc
     3481 catctg
//
LOCUS       DACAPB010000294         3481 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.300, whole genome shotgun sequence.
ACCESSION   DACAPB010000294 DACAPB010000000
VERSION     DACAPB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.300"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..140
                     /locus_tag="IT904_004564"
     CDS             <1..140
                     /locus_tag="IT904_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP3194233.1"
                     /translation="FKRKNESQLALDGGDNLAYIATSMVNLRLAQERYPDVQFHQTRE
                     H"
     gene            533..1138
                     /gene="osmY"
                     /locus_tag="IT904_004565"
     CDS             533..1138
                     /gene="osmY"
                     /locus_tag="IT904_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAP3194234.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            1265..1426
                     /locus_tag="IT904_004566"
     CDS             1265..1426
                     /locus_tag="IT904_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAP3194235.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            1548..2621
                     /locus_tag="IT904_004567"
     CDS             1548..2621
                     /locus_tag="IT904_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAP3194236.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRVPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            2618..3400
                     /locus_tag="IT904_004568"
     CDS             2618..3400
                     /locus_tag="IT904_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP3194237.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGILEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT          841 a          859 c          936 g          845 t
ORIGIN      
        1 agttcaagcg taagaacgaa agccaactgg cgcttgatgg cggcgataac ctcgcttaca
       61 tcgctaccag catggtcaac ctgcgcctgg cgcaggaacg ttatcccgac gttcagttcc
      121 accagacccg cgagcattaa ttcttgcctc cagggcgcgg tagccgctgc gccctgtcaa
      181 tttcccttcc ttattagccg cttacggaat gttcttaaaa cattcacttt tgcttatgtt
      241 ttcgctgata tcccgagcgg tttcaaaatt gtgatctata tttaacaaag taatgacatt
      301 tctgacggcg ttaaataccg ttcaatgcgt agatatcagt atctaaagcc gtcgattgtc
      361 attctaccga tattaataac tgattcagag gctgtaatgg tcgttattca tcacccatcg
      421 cttttgtgat ggcgaccatt gacttctgta gagggtgaag tctctcccta ttcagcaatg
      481 caacctcgtg ttgccaggct caaattacga gcaaacatac aggaataaat cgatgactat
      541 gacaagactg aagatttcga aaactctgct ggctgtaatg ttgacctctg ccgtcgcgac
      601 cggctctgcc tacgcggaaa acaacgcaca gactaccaat gaaagcgcag ggcaaaaagt
      661 cgatagctct atgaataaag tcggtaattt catggatgat agcgccatca ccgcgaaagt
      721 gaaggcggcc ctggtggatc atgacaacat caagagcacc gatatctctg taaaaaccga
      781 tcaaaaagtc gtgaccctga gcggtttcgt tgaaagccag gctcaggccg aagaggcagt
      841 gaaagtggcg aaaggcgttg aaggggtgac ctctgtcagc gacaaactgc acgttcgcga
      901 cgctaaagaa ggctcggtga agggctacgc gggtgacacc gccaccacca gtgaaatcaa
      961 agccaaactg ctggcggacg atatcgtccc ttcccgtcat gtgaaagttg aaaccaccga
     1021 tggcgtggtt cagctctccg gtaccgtaga ttctcaggca caaagtgacc gtgctgaaag
     1081 tatcgccaaa gcggtagatg gtgtgaaaag cgttaaaaat gatctgaaaa ctaagtaatt
     1141 cgtcgtaatt cgtcctcccg aactttgtcg ggaggcgcga tgtgcaccac actaaaaata
     1201 tcgcattgag tgattttcac gctcatatca agcggtgaca ttaactatgg taaaggagac
     1261 gcttatgttt cgttggggca tcatatttct ggttatcgcg ttaatcgccg ccgcacttgg
     1321 gtttggtggt ctggccggta ccgctgcagg cgcagctaaa attgtctttg tcgtcgggat
     1381 tattctgttc ctggtgagtt tgttcatggg ccgaaaacga ccctagattt cagaccatta
     1441 agaaaatttt atccaaagcc agtccagcgg actggctttt gcggttttag cgaataataa
     1501 attgcgttac tttgtcattc tttcacaaca aaaacaggaa ggcagaggtg gggcagcgaa
     1561 tacctgtaac gcttggtaat attgcgccgt tgtcgctaag gccgttccag cctggacgaa
     1621 tagctctggt gtgcgaaggc ggcggacagc gtggaatttt cacggctggc gtgctggatg
     1681 agtttatgcg cgcgcagttt aatcctttcg atctttatct cggcacatct gccggggcgc
     1741 agaacctctc ggcgtttatc tgcaatcagc ccggttacgc gcgcaaagtc atcatgcgct
     1801 ataccacaaa acgcgaattt ttcgatccat tgcgctttgt ccgtggagga aatcttatcg
     1861 atctcgactg gctggtggag gccaccgcaa gccagatgcc gttgcaaatg gataccgccg
     1921 cgcggttgtt tgacagcggc aaatcgtttt atatgtgcgc ctgtcgtcag gatgactacg
     1981 cgccgaatta ctttttacca accaaacaaa actggctgga tgtgattcgc gcctccagtg
     2041 cgatacctgg cttttatcgt agcggagtgt cgctggaagg cattaactac ctggatggcg
     2101 ggatcagtga tgcgattccg gttaaagagg cggcaaggca gggcgctaaa acgttggtcg
     2161 tcatccgcac tgtgccgtca caaatgtact acacgccgca gtggttcaaa cgcatggaac
     2221 gctggctggg tgacagtagc ctgcagccgc tggtcaatct ggtgcagcat catgaaacca
     2281 gctatcgtga cattcagcaa tttattgaga aaccaccggg caagctgcgg atattcgaaa
     2341 tttatccgcc gaagccatta catagtatcg cgcttggcag tcgggttccg gcgctgcgtg
     2401 aagactataa acttgggcgt ttatgcggtc gttatttcct cgccacggtt ggcaagctat
     2461 taactgaaaa agcgccgctt acccgccatc tggtgccagt ggtgacgccg gaatcgatcg
     2521 ttattccgcc tgcgccagtc gccaacgata cgctggttgc cgaagtgagc gatgctccgc
     2581 aggcgaacga cccgacattt aacaatgagg atctggcttg atttgccgtt ttatcgatac
     2641 ccactgccat tttgatttcc cgccgtttag tggcgatgaa gaggccagcc tgcaacgcgc
     2701 ggcacaagcg ggcgtaggca agatcattgt tccggcgaca gaggcggaaa attttgcccg
     2761 cgtgcaggcg ttagcggaaa aatatcaacc gctgtatgcc gcattggggt tgcatccggg
     2821 gatattggaa aaacatagcg atgtgtctct tgatcagcta cagcaggcgc tggaaaggcg
     2881 tccggcgaag gtggtggcgg tgggggagat cggtctggat ctcttcggcg acgatccgca
     2941 atttgagagg cagcagtggt tactcgacga acaactgaaa ctggcgaaac gctacgatct
     3001 gccggttatc ctgcattcac ggcgcacaca cgacaaactg gcgatgcatc ttaaacgcca
     3061 cgatttgccg cgtgctggtg ttgtccacgg attttctggc agccagcaac aggctgaacg
     3121 gtttgtacag ctgggctaca aaatcggcgt aggcggtact atcacctatc cacgcgccag
     3181 taaaacccgc gatgtcatcg caaaattacc gctggcatcg ttgttgctgg aaaccgacgc
     3241 gccggatatg ccgctcaacg gctttcaggg gcagcctaac cgaccggaac aggctgtgcg
     3301 ggtgtttgat gtgctttgcg agttgcgccc ggaaccagaa gatgagattg ccgaagtgtt
     3361 gcttaataat acgtatgcgt tgtttagcgt tagtgggtag tttacccttc gtaggcagca
     3421 tccggcagtc ggcgcataat gcctgatgcg acgcttgtcg cgtcttatca tgcctacagg
     3481 t
//
LOCUS       DACAPB010000295         3444 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.301, whole genome shotgun sequence.
ACCESSION   DACAPB010000295 DACAPB010000000
VERSION     DACAPB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.301"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..426
                     /locus_tag="IT904_004569"
     CDS             <1..426
                     /locus_tag="IT904_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAP3194238.1"
                     /translation="RLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGSFIKRGDMTMMVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            436..1416
                     /gene="ydjG"
                     /locus_tag="IT904_004570"
     CDS             436..1416
                     /gene="ydjG"
                     /locus_tag="IT904_004570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAP3194239.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARENKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            1553..2311
                     /locus_tag="IT904_004571"
     CDS             1553..2311
                     /locus_tag="IT904_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP3194240.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            2429..3444
                     /locus_tag="IT904_004572"
                     /pseudo
     CDS             2429..3444
                     /locus_tag="IT904_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          935 a          682 c          865 g          962 t
ORIGIN      
        1 cggttgaatg aaacgctgga tgatatttgc gaagcattga gttatgtcga ttatctgttt
       61 cctaattatg ccgaggcaaa attactcacc gggaaagaga cactggatga aattgctgac
      121 tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa cgggtaaaga cggcagcttt
      181 atcaagcgtg gtgacatgac gatgatggtg ccggcggtcg caggaataac cgccatcgac
      241 accattggcg cgggcgataa ctttgcttca ggttttattg cggcactgtt agaaggcaaa
      301 aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt tctaagcgtc
      361 ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga agaatacgaa
      421 ggataatgaa ggcaaatgaa aaagatacct ttaggcacaa cggatattac gctttcgcga
      481 atggggttgg ggacatgggc cattggcggc ggtcctgcat ggaatggcga tctcgatcgg
      541 caaatatgta ttgatacgat tcttgaagcc catcgttgcg gcattaatct gattgatact
      601 gcaccaggat ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt aaaaaaactg
      661 ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg tctgggaacg aaaaggaagt
      721 ttattcaaca aagttggcga tcggcagttg tataaaaacc tttccccgga atctatccgc
      781 gaagaggtag aagccagctt gcaacgtctg ggtattgatt acatcgatat ctacatgacg
      841 cactggcagt cggtgccgcc attttatacg ccgatcgctg aaactgtcgc agtgcttaat
      901 gagttaaagg ctgaagggaa aattcgcgcg ataggcgctg ctaacgtcga tgctgaccat
      961 atccgcgagt atctgcaata tggtgaactg gatattattc aggcgaaata cagtatcctc
     1021 gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg ataatggcat tgtggttcag
     1081 gtttattccc cgctagagca gggattgttg accggcacca tcactcgtga ttacgttccg
     1141 ggtggcgctc gggaaaataa agtctggttc cagcgtgaaa acatgctgaa agtgattgat
     1201 atgcttgaac agtggcagcc actttgtgct cgttatcagt gcacaattcc cactctggca
     1261 ctggcgtgga tattaaaaca gagtgattta atctccattc ttagtggggc tactgcaccg
     1321 gaacaggtac gcgaaaatgt cgcggcactg attatcaact tatcggatgc agacgcaaca
     1381 ttgatgaggg aaatggcaga ggccctggag cgttaaatac tcaacacgat tcacctctgt
     1441 ttctgtgaaa actgtgatga aaatctgtga ttcaaacagg ttgttttgaa agtaaacatc
     1501 ggttatgcga taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa tcgtggcggc
     1561 aaaagacagg attcaggcaa ttaagcaaat ggttgccaac gataaaaaag tgacagtctc
     1621 aaatttgagt gggatttttc aggtaaccga agaaaccatt cgccgcgatc ttgagaagct
     1681 ggaagatgaa ggctttttga ccagaaccta tggtggtgct gttttaaata cagcgatgtt
     1741 gacggagaat atccattttt ataagcgcgc ttcatcgttt tatgaagaga agcagcttat
     1801 tgcacgcaag gcactaccct ttatcgacaa taaaaccacc atggcagccg attccagtag
     1861 taccgtaatg gaattgctca aattgttaca ggaccgtagt gacctgacgt tgctaaccaa
     1921 ctcggcggaa gcgattcatg ttctggctca gtcagaaatt aaagtcgttt caacgggtgg
     1981 ggaactaaac aaaaatacac tttcactgca aggaagaatt actaaagaga tcatcagccg
     2041 ctatcatgtc gatatcatgg taatgagttg taaaggtctt gatattaaca gcggcgcgct
     2101 ggactctaat gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg cgacagaagt
     2161 tgcgttattg gttgatcact ctaagtttga tcgcaaagct tttgtccagt tagctgattt
     2221 tagtcatatt aattacataa taactgataa atcaccgggt gcagagtgga tagcattttg
     2281 caaagacaat aatatccagc tggtgtggta atttaagcac acgttgtcaa tattatttgt
     2341 tcctgcgcct tgaaataaaa tgatgataaa gatgcggcaa gtgaatttgc cgcaattgct
     2401 acctttttta gcaaatccga ggcacccaat ggaacaatat gatcaaattg gcgcaagact
     2461 ggaccgcttg cctttggccc ggtttcatta tcgtatattt ggtataataa gctttagtct
     2521 gttattaacg ggttttttga gttactccgg taatgttgtc ttagcaaagc tggtaagcaa
     2581 tggatggtca aataatttcc tcaatgccgc ctttacctcg gcattaatgt ttggttattt
     2641 tcatcggctc tcttaccggt gggttcatcg gtgattactt tgggcggcgc agggcgtttc
     2701 gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc cctgatatgt
     2761 actggctcat cttctttcgc ttcctgatgg gaacaggaat gggggcgctg attatggttg
     2821 gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg tccgcgcggc
     2881 tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggtgtg gtggttatcg
     2941 ctttttttag ttggcggata atgtttctgt tgggtggtat tggcatactg ttagcctggc
     3001 ttctctcagg taaatacttt attgagtcgc cacgatggct ggcagggaaa gggcaaatcg
     3061 caggtgcaga atgccaactt cgtgaagtag agcagcaaat tgaaagagag aagagtattc
     3121 gtttaccccc gcttacttcg tatcagagca acagcaaagt taaagtaatc aagggtactt
     3181 tctggctcct gtttaaaggt gaaatgttac gacgtacatt agtcgcgatt actgttttaa
     3241 ttgcaatgaa catttcgctt tataccatca ccgtatggat accgaccata tttgttaact
     3301 ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt ggcgctccgg
     3361 taggaatatt tattgcggca ttaattattg atcattttcc tcgtcggtta tttggctcca
     3421 ccttacttat tattattgcc gtgt
//
LOCUS       DACAPB010000296         3379 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.302, whole genome shotgun sequence.
ACCESSION   DACAPB010000296 DACAPB010000000
VERSION     DACAPB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.302"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(319..1323)
                     /locus_tag="IT904_004573"
     CDS             complement(319..1323)
                     /locus_tag="IT904_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAP3194241.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(1741..3177)
                     /locus_tag="IT904_004574"
     CDS             complement(1741..3177)
                     /locus_tag="IT904_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011790215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAP3194242.1"
                     /translation="MSTLDVQKLKDDAVEWALTHGVAFKESSYSAVHTPFTLTPTPIS
                     RKSYQYLKNATGILSKLIYSVSEDHDFLYSAIYPIKAGNAFFSALLNMHQQIHSSSRH
                     APRLPLLIMRSDFMDDNRDGFRLIEFNGIAAGMGPFGQRIHDLHHYLQRQYPAVFSDL
                     SNQATGALVDNQAIEGLSAAICQATCRIKQEFGDPGPARFLMIVQENENNVFDQHLLE
                     LALQQKGIETHRRTFRELHNQLSTGADNRLILEGVGPLDTVYLRAGYQYSDYEAFNID
                     EQECCQALMATRVFIEQHRVAVNATVGQQLATSKRVQMLLSQLGYDEFVAFGLTLQEA
                     KIAKTILGEMLPISPDSINLAKESPSETWVLKNQGEGGGHCLFGADILTKLTELTPQQ
                     YQSWSLMRRLHPQPRAMPTLIVRKGELHKVNDLISELGMFSVQTDNNPSSAEHSFAGY
                     LIRSKSAESTEGGVHSGQGVLDSLVYSD"
BASE COUNT          854 a          840 c          760 g          925 t
ORIGIN      
        1 gcaccggcgc ccggcaaggt gggcggattt ccacacagca cggggtccgc ttggaaaacg
       61 gaatctatgg tcactcccgt ttttgcaaca ccgattttga cgacaagttg gcttgcttga
      121 atctatccgg cgtctgaatg ggattttatt cccgcgcctc gatgagttcc gcgcctgatg
      181 aacctccaga aaatatacgg cttcaatgag cctttccgtt ttacaggttc ctcaacaggc
      241 cggtgggccg ttagtatcat caatatcagt attcgcaaaa ccagatgaat gattgtttaa
      301 actggtgtat ttctgccttt atgcttcgta agtttgctgt cgcgccgtca gtgcccaggc
      361 tattctggcc agcttgtttg ccagagcaca ggtgacgaca aagttgcttt tccgacacaa
      421 caactccctg acccagtcgg ccaacttgcc agactggtgt tccagttttt gtatgaatac
      481 cctggcacac tgaaccaaca aagttcggat ctttttgttg ccccgcttgc taatccctaa
      541 caatgtcgtc cgacctcccg tgctgtactg tcggggtacc agccctgttg ccgccgcaaa
      601 gtcacggctg ctggcgtact gcttcccgtc gccaatctca gttgaaatag tactggcagt
      661 cagcgttcca acgcagggaa tactcagcaa gcgctgtcca acctcatctt cgtccaactt
      721 tcgtttcaac tgagattcca gatctttaat ctgctcaaca agatagtgat aatgctgttg
      781 taatttcagc agtaactggc tgagataaag aggcaaacta ctgtcctcaa gaagggtact
      841 cagtcgacta ataacggcag cacctcgcgg aacgctgata ccaaattcca gcagaaaagc
      901 atgcatctga ttagttgttt tcaccttatc ctgaaccagg gattcacgga cacgatgcag
      961 agctcgcatt gcctgctgag attcggttct gggctgcacg aaacgcatag atggacgtga
     1021 tgctgcttca cagatagctt cagcatcaac gaagtcattt ttgttgcttt taacgaatgg
     1081 gcggacaaat tgcggtgata tcagctttgg aaaatgccct aactcttcca gcttgcgtgc
     1141 cataaagtga gaaccgccac aggcttccat cgcgatggtt gttgccgggc atgtcgccag
     1201 aaattcgatt agctttggtc gggtgaattt tttacggtaa acggccttcc cacgatgatc
     1261 ctgacaatga atatggaaag agttcttacc cagatcgata ccaataagcg caatgttttc
     1321 catgatggtt ctccgaatga aagcctgtcc tcagcatagt actgggaagg agggagtgac
     1381 catctcatta aatatcccac gctaagactg tttgctgtac aaattgatgt cgacagcgcg
     1441 taatgaccgt agtcacagga acagctaatg gtattgaaaa ttgacccgta actgaaacag
     1501 catcgaccca gcagcgatga tgtgaacctt gaagaacttt gggtgcagcc tcttatagca
     1561 gcagtttttg ctgttgttcc cttaataaga tatgccctgt aaataccctc atggcttcat
     1621 ttataatact attattagct tactacgact cagtctagaa acagcatcat ctccactggg
     1681 gctaaaacac cacttaccgc atgctttcat ataaccagat ttcagccccc ttgaaaggga
     1741 ctaatcgctg tataccagcg agtctaaaac accttgtcca ctatggaccc ctccctctgt
     1801 cgattcagct gatttgctac gaattagata tccagcaaaa ctgtgctcag cactggaggg
     1861 attgttatct gtctgcacgg agaacattcc aagttcgctt atcaaatcat tcaccttatg
     1921 caactcccct ttacgcacaa tcaaggtggg catagctcgg ggctgagggt gtaaacggcg
     1981 catcagcgac caggactgat attgttgtgg agtgagttcc gtcagttttg tcaggatatc
     2041 cgcaccaaac agacaatgcc ccccgccttc accctgattt ttgagaaccc aggtttccga
     2101 aggagactct tttgcaaggt tgatactatc cgggctgatg ggtaacatct cacccagtat
     2161 cgtttttgct attttagctt cctgcagtgt taatccaaat gcgacaaact cgtcgtaacc
     2221 caattgcgag agcagcattt gtactcgctt gctggtcgcc agttgctggc caactgtggc
     2281 gttcactgca acccggtgct gctcaatgaa tactcgggtc gccatcagcg cctgacaaca
     2341 ctcctgctca tcgatattga atgcttcata atcagaatac tgatatccgg cacgtaagta
     2401 gacggtatca agtgggccta caccctcgag aatgagcctg ttgtcggcgc ctgtactcaa
     2461 ttgattatgc agttcacgaa aagtcctgcg atgcgtttcg attccttttt gctggagggc
     2521 gagctcaagc aagtgttgat caaacacatt attttcattc tcctgcacaa tcatcaggaa
     2581 cctggcaggg ccggggtctc cgaactcctg cttaattctg caagttgcct gacaaatcgc
     2641 tgctgataac ccttcgatcg cttgattgtc gacaagcgcc cctgttgcct gattcgataa
     2701 atcagaaaag acagcgggat attgccgttg tagatagtga tgcagatcat gtatccgctg
     2761 accaaacggc cccattcctg cagctatacc attgaactct ataagcctga acccatcacg
     2821 attatcatcc ataaagtcgc tacgcataat gagtaatggt aatcgaggag catgccttga
     2881 tgatgaatgg atttgttgat gcatgttcag gagggctgaa aagaaggcat tcccggcctt
     2941 gatgggataa atcgcagagt acaaaaaatc atgatcctca gaaactgaat agattaattt
     3001 gctgagtatt cctgttgcat tctttaagta ctgataggat ttacggctga taggtgttgg
     3061 tgtcagggta aacggggtat gcactgcgga atacgacgat tccttgaaag caacaccatg
     3121 agttaaggcc cattcgacgg catcatcctt gagcttctga acatcaagag tactcatttt
     3181 acgtcccctg attttacctg gcatatcatt ggtggttgtt acttggctat cagcctataa
     3241 aatttgattg attacttcaa tagttcacgt ataggaaatt gaaaaaccag tcgataccat
     3301 tgtccacgcc ttatgccacg tgagcctttc tgcttttccc accactccgg tggtttccct
     3361 ctctgtatgg tcgattttt
//
LOCUS       DACAPB010000297         3360 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.303, whole genome shotgun sequence.
ACCESSION   DACAPB010000297 DACAPB010000000
VERSION     DACAPB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.303"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..2006)
                     /locus_tag="IT904_004575"
     CDS             complement(<1..2006)
                     /locus_tag="IT904_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAP3194243.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNAL"
     gene            2124..3317
                     /locus_tag="IT904_004576"
     CDS             2124..3317
                     /locus_tag="IT904_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP3194244.1"
                     /translation="MRACINNQQIRHNNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWVTSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
BASE COUNT          797 a          887 c          907 g          769 t
ORIGIN      
        1 atcagagcgt taacgtcgcg tccctgacac agaccgcgcc actgcgcgag ggctgattcg
       61 ctacgaccat cgaaccagtt cagccaccac agagtggcgt tcagcggata atcgcgcagc
      121 agtttctcgc gctgcaaacg cgcgttgtcc tgacgaccac ttaacaccag cagcaggtta
      181 tgcaggcaaa gcacttcctg attggttggg caggcgcgaa gactttgttg gcaaaaatcc
      241 agacccgcgt cgaagttacc attacgcgca gccagtcgtg ccaggccata atagccaccg
      301 gctttgctgt tgccgctcca gaccgcccgc cagaaatcct cttcggcttg ttgatattgt
      361 ccctgacgtt cgtaagcact ggcgcgaatc aaactcgcct gtccgcactg cggatttttg
      421 ttcagcgcat gtgcgcgttt cagagcctga gtggcatacg ccaccgcttg cgggaaatcg
      481 gcacggttat attccagcat cgccagcgcc aggttacagc ggtaatccag cggatccagc
      541 gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga
      601 tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct
      661 ttggcgacgt ccggcaacgg caactcttgc ggctgatgtt cctgataact cagtacaata
      721 ttgccgtcgg catcggagag ctcaatggtc agcctttcag gattgatacc gtgcaacacg
      781 ccctggatgg cggtcgcagg catcagtgcc acggcatcat caagtaacgc gttgcatttg
      841 ccgatttcgc ggatcgccag gcggtatccg ttcaacggag agatggcata cagcccccac
      901 tcaatccccc gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc
      961 atgcccagag aatgataagg caggaaatac tgctcgaaac gcttctcttc gtaagcatca
     1021 agccaggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg
     1081 ccgttattgt cggtcaggct cttgtcccac gcctggccaa attcactgtg tccccaactc
     1141 cactgttttt tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc gccatcttca
     1201 tcgtgacacc acgcgccaac aaaatcgtac tgtgattttt cagccatata tgaggttgga
     1261 acgggcacat ttttatagcg agaaatgtcc actccagcgg agtagtccac tttgtagtaa
     1321 gtgccggtgg cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgccgtt
     1381 acatccggcg ggaaaacgct ctgatgccct tccccccctt tcactgccgg gttggcccac
     1441 cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat ttccagcgcc
     1501 gcccggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat cggctccgtt
     1561 tcgccgaccc acaccgtctg tgcgccgtct tcatgggctt cgagggtgaa atcaacgggc
     1621 ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccacc ggagatccac
     1681 ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgataaac aaaatcgcgt
     1741 tgtttcactt tatcccatgc gcgatgcaca cgaccgccca gttccggcag gatcatcact
     1801 ttgatgtagt cgttttccag ccacaccgcc tgccaggatt tcagggtttt ctgctcgctc
     1861 agcgtatcgg tcacgccata gggataaacc gcgccggacg atccctgata aacgcgattt
     1921 tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc
     1981 tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta
     2041 aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat
     2101 taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccaca
     2161 ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc
     2221 tggcccggct ggcgcaaatt tcgattccgg cggtcagtaa tattttgcag gaactggaaa
     2281 gcgaaaaacg ggtggtgaat atcgacgatg aaagtcagac gcgcgggcat agtagcggta
     2341 catggctgat tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta
     2401 ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg cgagtttgag tatttacaga
     2461 ttgatgcacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc
     2521 gtaaattgtg gccggaccgt accatcaacc tggcgctggc aatccacggt caggttgatc
     2581 cggtgaccgg cgtgtcgcaa accatgccgc aagcgccgtg gacaacgccg gttgaggtaa
     2641 agtatctgct ggaagagaag ctcggcattc gggtgatggt cgataatgac tgcgtgatgc
     2701 tggcgctcgc ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg
     2761 ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt
     2821 atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg
     2881 gacgttacgg ctgcctggaa accgtcgcct cgttaagcgc attaaaaaaa caggcgcggg
     2941 tatggctaaa atcacaaccg gttagtactc aacttgatcc tgaaaaactg actacagcgc
     3001 agttaatcgc tgcctggcaa agtggagaac cgtgggtcac cagctgggtt gaccgcagtg
     3061 ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt
     3121 ggttgtacgg tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga
     3181 caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct
     3241 tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc
     3301 agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga agccttgatg
//
LOCUS       DACAPB010000298         3146 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.304, whole genome shotgun sequence.
ACCESSION   DACAPB010000298 DACAPB010000000
VERSION     DACAPB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.304"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..859)
                     /locus_tag="IT904_004577"
     CDS             complement(<1..859)
                     /locus_tag="IT904_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAP3194245.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNP"
     gene            complement(1044..2132)
                     /gene="ompF"
                     /locus_tag="IT904_004578"
     CDS             complement(1044..2132)
                     /gene="ompF"
                     /locus_tag="IT904_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAP3194246.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADIGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(2735..>3146)
                     /locus_tag="IT904_004579"
     CDS             complement(2735..>3146)
                     /locus_tag="IT904_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP3194247.1"
                     /translation="VYWGVDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDG
                     KTVAAMDVLAPGIGEIIGGSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHS
                     GFGLGFERLIAYVTGVQNVRDVIPFPRTPRNASF"
BASE COUNT          853 a          733 c          724 g          836 t
ORIGIN      
        1 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
       61 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      121 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      181 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      241 accagccttt ctcaacggag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      301 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcat
      361 tcaggctgtt aatcagtgca tcgaagtcaa gagtgtgatt ttccgcatca taataagcgt
      421 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      481 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      541 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
      601 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
      661 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
      721 tcagtaccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
      781 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
      841 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
      901 agggtaacgg gagatttaca aaattccaac tattactgat gaaaactcag gctgtttttg
      961 caagacgtga gattgctctg gaaggtataa aaaaaacagg accaacgtcc tgttttttcg
     1021 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1081 tctgaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1141 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1201 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1261 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1321 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1381 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1441 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1501 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1561 gagcggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     1621 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     1681 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     1741 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaat gtcagcgtat
     1801 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     1861 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttgt
     1921 gtttcccctt taaaaccaag acgggcataa gttttgtcgc cgtttccgcc gtaactgttt
     1981 acaccattgt ctttagaaaa ataatgcaga ccgacagctt taccgtacag atctactttg
     2041 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2101 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2161 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2221 agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt ttcgctaccg
     2281 tgaaagtgct acaaagataa agatttggtt tcaaaaagaa aaaaatatgt aaccaaaagt
     2341 aaaatttaag gaactttgtg aacaccgtca tatttccata gaagcgtgat gatatttaca
     2401 gcaattttta tctatttata tgattttctt atatttaaat taactaaacg gaaattttgt
     2461 ttctgatgga aactttatcg acctggcact aaatcctcgt tcagacatcc agaatgccaa
     2521 aaaatagtat gaaaattgtg ctatcagcta agaaaaaact aatccgcaat aagaatccag
     2581 gttatttttt gtgcgtaaat gttgcaatgt aatattccgt aacaggatga tcgttatggc
     2641 ggggttgcaa atagattgct tgacttaaca aacagataac ttgacagaaa ggataaaaaa
     2701 gagccagcag gcgctggctc tttgaggaag aaaatcagaa gctggcgttg cgcggagtac
     2761 gtgggaacgg aatcacatca cgcacgtttt gcacgccagt tacgtaagca atcaggcgtt
     2821 caaaaccaag accgaaaccg gaatgcggaa cagtaccgta gcgacgcaga tcgcgatacc
     2881 accagtaatc ttctttgttc aggcccattt ccagcatacg ctcgtccagc acgtccagac
     2941 gctcttcacg ctgggagcca ccaatgatct caccgatgcc cggagccaga acgtccatag
     3001 ccgcaacggt tttaccgtct tcgttaaggc gcatatagaa cgctttaata tctttcggat
     3061 agtttttaac cactaccgga gctttaaagt gttcttccgc cagataacgc tcatgctcag
     3121 aagagagatc aacgccccag taaact
//
LOCUS       DACAPB010000299         3113 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.305, whole genome shotgun sequence.
ACCESSION   DACAPB010000299 DACAPB010000000
VERSION     DACAPB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.305"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1677
                     /locus_tag="IT904_004580"
     CDS             <1..1677
                     /locus_tag="IT904_004580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP3194248.1"
                     /translation="AQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPES
                     PNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYD
                     STTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVNKGDALTLVELTPA
                     QHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEI
                     VTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPE
                     EGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENE
                     VLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEF
                     RIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVP
                     LPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLA
                     DAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK"
     gene            1887..2861
                     /gene="cysB"
                     /locus_tag="IT904_004581"
     CDS             1887..2861
                     /gene="cysB"
                     /locus_tag="IT904_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAP3194249.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          838 a          716 c          772 g          787 t
ORIGIN      
        1 gcgcagcgtt tgtatgaagc aggctatatc acttacatgc gtaccgactc cactaacctg
       61 agtcaggacg cggtaaatat ggttcgcggt tatatcagcg ataattttgg taagaaatat
      121 ctgccggaaa gtccgaatca gtacgccagc aaagaaaact cacaggaagc gcacgaagcg
      181 attcgtcctt ctgacgtcaa tgtgatggcg gaatcgctga aggatatgga agcagatgcg
      241 cagaaactgt accagttaat ctggcgtcag ttcgttgcct gccagatgac cccagcgaaa
      301 tatgactcca cgacgctgac cgttggtgcg ggcgatttcc gcctgaaagc acgcggtcgt
      361 attttgcgtt ttgatggctg gacaaaagtg atgcctgcgt tgcgtaaagg cgatgaagat
      421 cgcatcttac cagcagttaa taaaggcgat gctctgacgc tcgttgaact gacaccagcc
      481 cagcacttta ccaagccgcc agcccgtttc agtgaagcat cgctggttaa agaactggaa
      541 aaacgtggta tcggtcgtcc gtctacctat gcgtcgatca tttcgaccat tcaggatcgt
      601 ggctatgtgc gagtagaaaa tcgtcgtttc tatgcggaaa aaatgggcga aatcgtcacc
      661 gatcgcctgg aagagaattt ccgcgagtta atgaactacg acttcaccgc gcagatggaa
      721 aacagccttg accaggtggc aaatcacgaa gcagagtgga aagctgtact ggatcacttc
      781 ttctcggatt tcacccagca gttagataaa gctgaaaaag atccggaaga gggtggtatg
      841 cgcccgaacc agatggttct gaccagcatt gactgcccga cttgtggtcg caaaatgggg
      901 attcgcacag cgagcaccgg ggtattcctt ggctgttctg gctatgcgct gccgccgaaa
      961 gagcgttgca aaaccaccat taacctggtg ccggaaaacg aagtgctgaa cgtgctggaa
     1021 ggcgaagacg ctgaaaccaa cgcgctgcgc gcaaaacgtc gttgcccgaa atgcggcacg
     1081 gcgatggaca gctatctcat cgatccgaaa cgtaagttgc atgtctgtgg taataaccca
     1141 acctgcgacg gttacgagat cgaagagggc gaattccgca ttaaaggtta tgacggcccg
     1201 atcgttgagt gtgaaaaatg tggctctgaa atgcacctga aaatggggcg tttcggtaaa
     1261 tacatggcct gcaccaacga agagtgtaaa aacacacgta agattttacg taacggcgaa
     1321 gtggcaccac cgaaagaaga tccggtgcca ttacctgagc tgccgtgcga aaaatcagat
     1381 gcttatttcg tgctgcgtga cggtgctgcc ggtgtgttcc tggctgccaa cactttcccg
     1441 aaatcgcgtg aaacgcgtgc gccactggtg gaagagcttt atcgcttccg cgaccgtctg
     1501 ccggaaaaac tgcgttatct ggccgatgcg ccacagcagg atccggaagg taataagacc
     1561 atggttcgct ttagccgtaa aaccaaacag caatatgtct cttcggaaaa agacggaaag
     1621 gcgactggct ggtcagcatt ttatgttgat ggcaaatggg ttgaaggaaa aaaataacct
     1681 ttaattctgt caggttttta taaacaaagg gtcgcgaaag cggccctttt ttattgcata
     1741 ttattttttc ttcacaccta tacactaagg ctataaatga tatagtggtt atagttagca
     1801 ccttttttat tattaaatcg tattagtcac ccgccaggtg tgacgaaaaa acgatgttct
     1861 gatggcgtct aagtggatgg tttaacatga aattacaaca acttcgctat attgttgagg
     1921 tggtcaatca taacctgaat gtctcatcaa cagcggaagg actttacaca tcacaacccg
     1981 ggatcagtaa acaagtcaga atgctggaag acgagctagg cattcaaatt ttttcccgaa
     2041 gcggcaagca cctgacgcag gtaacgccag cagggcaaga aataattcgt atcgctcgcg
     2101 aagtcctgtc gaaagtcgat gccataaaat cggttgccgg agagcacacc tggccggata
     2161 aaggttcact gtatatcgcc accacgcata cccaggcacg ctacgcatta ccaaacgtca
     2221 tcaaaggctt tattgagcgt tatcctcgcg tttctttgca tatgcaccag ggctcgccga
     2281 cacaaattgc tgatgccgtc tctaaaggca atgctgattt cgctatcgcc acagaagcgc
     2341 tgcatctgta tgaagattta gtgatgttac cgtgctacca ctggaatcgg gctattgtag
     2401 tcactccgga tcacccgctg gcaggcaaaa aagccattac cattgaagaa ctggcgcaat
     2461 atccgttggt gacatatacc ttcggcttta ccggacgttc agaactggat actgccttta
     2521 atcgcgcagg gttaacgccg cgtatcgttt tcacggcaac ggatgctgac gtcattaaaa
     2581 cttacgtccg gttagggctg ggggtagggg tcattgccag catggcggtg gatccggtcg
     2641 ccgatcccga ccttgtgcgt gttgatgctc acgatatctt cagccacagt acaaccaaaa
     2701 ttggttttcg ccgtagtact ttcttgcgca gttatatgta tgatttcatt cagcgttttg
     2761 caccgcattt aacgcgtgat gtcgttgatg cggctgtcgc attgcgctct aatgaagaaa
     2821 ttgaggtcat gtttaaagat ataaaactgc cggaaaaata atttcttgcg ttattttcgg
     2881 caccttttat gtagcgaagg tgccggaata tattctcttt tgttacttat ttctataccg
     2941 tgcacaatat ttttatccct catatttata gggtaaatta cctgtaaagt agtgtctaat
     3001 tacccgcctc gcaaactcca gttttcaaat atctatttca ggtcaaaaga ttgaattatt
     3061 cactggagac gattcgtaaa ttcgctggat ttttgactaa agtttcttta gga
//
LOCUS       DACAPB010000300         3105 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.306, whole genome shotgun sequence.
ACCESSION   DACAPB010000300 DACAPB010000000
VERSION     DACAPB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.306"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            156..3029
                     /gene="gcvP"
                     /locus_tag="IT904_004582"
     CDS             156..3029
                     /gene="gcvP"
                     /locus_tag="IT904_004582"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAP3194250.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
BASE COUNT          719 a          850 c          868 g          668 t
ORIGIN      
        1 tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg ctttattcct cttctgcggg
       61 agaggatcag ggtgaggaaa atttatgcct caccctcact ctcttcgtaa ggagagaggt
      121 tcacaattca ctgcacgttt caggaaccat cgctcatgac acagacgtta agccagcttg
      181 aaaacagcgg cgcttttatt gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa
      241 tgctgaatgc cgttggtgca caatcgttaa acgcgctgac cggccagatt gtgccgaaag
      301 atattcagct tgcgacacca ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg
      361 cagaactcaa ggctattgcc agtcgcaata aacgcttcac gtcttacatc ggcatgggtt
      421 acaccgccgt gcagctaccg ccggttatcc tgcgtaacat gctggaaaat ccgggctggt
      481 ataccgcgta cactccgtat caacctgaag tctcccaggg ccgccttgaa gcactgctca
      541 acttccagca ggtaacgctg gatttgactg gactggatat ggcctctgct tctcttctgg
      601 acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa
      661 atgccaaccg cttcttcgtg gcttccgatg tgcatccgca aacgctggat gtggtccgta
      721 ctcgtgccga aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc
      781 atcaggacgt cttcggcgtg ctgttacagc aggtaggcac taccggtgaa attcacgact
      841 acactgcgct tattagcgaa ctgaaatcac gcaaaattgt ggtcagcgtt gccgccgata
      901 ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt gtttttggtt
      961 cggcgcaacg cttcggcgtg ccgatgggct acggtggccc acacgcggca ttctttgcgg
     1021 cgaaagatga atacaaacgc tcaatgccgg gccgtattat cggtgtatcg aaagatgcag
     1081 ctggcaacac cgctctgcgc atggcgatgc agactcgcga gcaacatatt cgtcgtgaga
     1141 aagcgaactc caacatttgt acttcccagg tactgctggc aaacatcgcc agcctgtatg
     1201 ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg
     1261 atatcctggc ggcaggcctg caacaaaaag gtctgaagct gcgccatgcg cactatttcg
     1321 acactctgtg tgtggaagtg gccgacaagg cgggcgtact gacgcgtgcc gaagcggctg
     1381 aaatcaacct gcgtagcgat atcctgaacg cggttgggat cacccttgat gaaaccacca
     1441 cgcgcgaaaa cgtgatgcag cttttcagcg tactgctggg ggataaccac ggcctggaca
     1501 tcgacacgct ggacaaagac gtggctcacg acagccgctc tattcaacct gcgatgctgc
     1561 gcgacgacga aatcctcacc catccggtgt ttaatcgcta ccacagcgaa accgaaatga
     1621 tgcgctatat gcactcgctg gagcgtaaag atctggcgct gaatcaggcg atgatcccgc
     1681 tgggttcctg caccatgaaa ctgaacgccg ccgccgagat gatcccaatc acctggccgg
     1741 aatttgccga actgcacccg ttctgcccgc cggagcaggc cgaaggttat cagcagatga
     1801 ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc
     1861 cgaactctgg cgcacagggc gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa
     1921 gccgcaacga agggcatcgc gatatctgcc tgatcccggc ttctgcgcac ggaactaacc
     1981 ccgcttctgc acatatggca ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca
     2041 acatcgatct gactgatctg cgcgcgaaag cggaacaggc gggcgataac ctctcctgta
     2101 tcatggtgac ttatccttct acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg
     2161 aagtcgtgca tcagttcggc ggtcaggttt accttgatgg cgcgaacatg aacgcccagg
     2221 ttggcatcac ctcgccgggc tttattggtg cggacgtttc acaccttaac ctacataaaa
     2281 ctttctgcat tccgcacggc ggtggtggtc cgggtatggg accgatcggc gtgaaagcgc
     2341 atttggcacc gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc
     2401 agggcgcggt ttctgcggca ccgttcggta gcgcctctat cctgccaatc agctggatgt
     2461 acatccgcat gatgggcgca gaagggctga aaaaagcaag ccaggtggca atcctcaacg
     2521 ccaactatat tgccagccgc ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg
     2581 gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct gaaagaagaa accggcatca
     2641 gcgagctgga tattgccaag cgcctgatcg actacggttt ccacgcgccg acgatgtcgt
     2701 tcccggtggc gggtacgctg atggttgaac cgactgaatc tgaaagcaaa gtggaactgg
     2761 atcgctttat cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg
     2821 tctggccgct ggaagataac ccgctggtga acgcgccgca cattcagagc gaactggtcg
     2881 ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt cccggcaggt gtggcagaca
     2941 aatactggcc gacagtgaaa cgtctggatg atgtttacgg cgaccgtaac ctgttctgct
     3001 cctgcgtacc gattagcgaa taccagtaat tcactgattc gactattttc taaaggcgct
     3061 tcggcgcctt tttagtcaga tgacaaagta caaaagtgct cagac
//
LOCUS       DACAPB010000301         3070 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.307, whole genome shotgun sequence.
ACCESSION   DACAPB010000301 DACAPB010000000
VERSION     DACAPB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.307"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..532)
                     /locus_tag="IT904_004583"
     CDS             complement(<1..532)
                     /locus_tag="IT904_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HAP3194251.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQM"
     gene            complement(688..1347)
                     /locus_tag="IT904_004584"
     CDS             complement(688..1347)
                     /locus_tag="IT904_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP3194252.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(1430..2242)
                     /gene="truA"
                     /locus_tag="IT904_004585"
     CDS             complement(1430..2242)
                     /gene="truA"
                     /locus_tag="IT904_004585"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP3194253.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(2242..>3070)
                     /locus_tag="IT904_004586"
     CDS             complement(2242..>3070)
                     /locus_tag="IT904_004586"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP3194254.1"
                     /translation="DWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFALEPDV
                     PLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLISASAQ
                     GKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVDEVRK
                     ILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEENEFP
                     TQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQEYLY"
BASE COUNT          750 a          886 c          729 g          705 t
ORIGIN      
        1 ccatctgcat cagcgacatc agtgcttcct gcatacgtgc gccaccagag gcggagaagc
       61 agatcagcgg gcagttatct tccagcgcct gctcaacggc acgcacgaaa cgtgcaccca
      121 caacagaccc cattgaaccg cccataaagg cgaactcgaa tgccgcagcg acaaccggca
      181 ttccatacag agtgcctttc atcaccacca gcgcatcttt ttcgccggtt tctttctgcg
      241 cagatgccag acggtcttta tacttcttgg agtcacgaaa cttcagcaca tctttcggct
      301 caagctcgct acccagctcc acaaggcttc cttcatctaa caggctatgc aggcgattac
      361 gcgctgtcat acgcatgtga tggtcacact tcggacagac ctcaagatta cgttccagct
      421 cagcgcggta taaaacctga ccgcagctat cacacttagt ccacacccct tcaggaatgc
      481 tcgccttgcg ggtgggagta atgttgcttt taattcgttc aatccagctc attagggacc
      541 tttctgtctg aacctggttc gatgccagtt ttatctttgg ggacgcataa tgccattttt
      601 gcccccaaca gaccatgaat gttgcacatt aaaacataac agcccgaaac tttggataaa
      661 aaagtggtcg aaccgcggag ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg
      721 acggattatt tcgatgacgc ccggcaaaat agaaaccaca ataatcccga cgatcagcag
      781 cttaaggtta tcctgaacca tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag
      841 cagtacccac aacagtgcgc cgatcacgtt ataagcggcg aaatgacggt acgacatgtg
      901 gcccattccc gcaacaaacg gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat
      961 aatcgttttg ccgccatgtt tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg
     1021 gaaaattttc gaattgggat tactgaacaa cttttcaccg aacaaccgcc cgatagtgta
     1081 attgaccgcg tcacccacaa tcgcggcgat cagcatcagc accaccatca tatggacgtt
     1141 gagatcgttg gtttccagtg atgccaacgc tccggcaaca aacagcaatg aatcacccgg
     1201 taaaaacggc gttaccacca gaccggtttc acagaacaaa attaagaaca aaatggcata
     1261 aacccagacg ccgtactccg cgaccagctc tgccagatgc acgtcaatgt gcaggataaa
     1321 atcgatgagg aaataaatca ggtccatatt tgcctatgcc ttgtactcgt catctttcag
     1381 gctgtaacta cgttggctac agcctgaaat gttccgagca ttattctcgt tagtccgcca
     1441 gaaatagcgg gcccattggc ggttttggaa gatcataccg gtcagggtaa tccaccgcga
     1501 ccagatacag cccttccgct tttgccgttg ctgccgccag cgttctgtcc tttgccgcca
     1561 gcaactctgc tatccagctc tccggctggt tgtgggcacc gacttccatc aggctgccga
     1621 caatattcct gaccatatga tgtacaaaag cattcgcttt aatatctacc accacataag
     1681 gaccgtgacg cgtgacatta atatgcataa cgttgcgcca cggggttcgg gactggcact
     1741 gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa gcattgcgca gcccgatgca
     1801 tccgttcagc gtccagcggt tcgtaaaaat gggttacccc tttactcagt accgccgggc
     1861 gcagccgatg attgtagatg atgtagcgat aacggcgagc cgtggcgcta aatcgggcat
     1921 gaaaatcatc aggtacagct ttaacccaac gcacagcgat gtcaccaggt aaattcgcat
     1981 ttacgcccaa ggtccacgcc gcgtctttgc gcagcgcggt tgtttcgaaa tgcacaacct
     2041 gcccggtacc gtgtacccct gcgtcagtac gcccggcgca gaagacggtg atgggttcgt
     2101 tcgccacctg ggagagcgcc ttttccagct tctcctgcac actgcgaact tcgttctgcc
     2161 gttgccagcc gtaatactta ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg
     2221 gcggttgttg ctggtcggac attagtacag atactcctgc accagtttct cggcgatttt
     2281 tactgccatc agcgcgccgc caaagcgaac gttatcggcc accgaccaga actggacttg
     2341 ctccggcata ccgtagtcat tacgcacgca gccaacagaa agatgcggcg tacccgaagc
     2401 atcacctacc tgagttggga attcgttctc ttcagagagc acaatatctt cgccttgaac
     2461 aaacgcatca cgcgcttctt ctgctgccag tggacgcaga gcttcaaagt tgaccatctg
     2521 ggcatgaccg tagaataccg gtgcctggac gacgctagcc gaaatcatca gcccttcgtc
     2581 ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct tcacgcacgc taccttcgct
     2641 atccggcagt aacggcagca tgttgaacgc cagctgacgc ccgaagaaat cttcttcgtc
     2701 aatcggaatg ccgttgagca atttcgcact ctgccccgct aacgcatcga ccgctttttt
     2761 gccctgggcg gaggctgaaa tcaggctggt aacgctgata cgtgataaac cgccctgatc
     2821 gattaacggt ttcagtgccg ccagcagctg gctggtcaga ctgtctggta cggcgatgac
     2881 attccggttc cggtaatctg tcagtacaaa cgggtttact tccggcacca ccagcggtac
     2941 gtcgggttcg agagcaaaca atccactgct gtcgatcacc aggcaacctg agttggtcgc
     3001 ttcttcaacc caggcagcgg tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg
     3061 cgtccagtcg
//
LOCUS       DACAPB010000302         2972 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.308, whole genome shotgun sequence.
ACCESSION   DACAPB010000302 DACAPB010000000
VERSION     DACAPB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.308"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(291..1262)
                     /locus_tag="IT904_004587"
     CDS             complement(291..1262)
                     /locus_tag="IT904_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAP3194255.1"
                     /translation="MKRAPVIPKHTIKTQPLEDTPSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRIPTECIERIEAILKELEKAAL"
     gene            complement(1262..2428)
                     /gene="sopA"
                     /locus_tag="IT904_004588"
     CDS             complement(1262..2428)
                     /gene="sopA"
                     /locus_tag="IT904_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAP3194256.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(2728..>2972)
                     /locus_tag="IT904_004589"
     CDS             complement(2728..>2972)
                     /locus_tag="IT904_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP3194257.1"
                     /translation="SLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLN
                     LRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          823 a          742 c          636 g          771 t
ORIGIN      
        1 acaatacgag tgggaccgcg gtcccagact gattagcaga ctaacaatat gagtgggccc
       61 gtggtcccag accgataatc agaccgacaa tatgagtggg accgtggtcc cagactgatt
      121 atcagaccga caatatgagt gggaccgtgg tcccagactg attatcagac cgacaataca
      181 actgggaccg tggtcccaga tataatattc aggccagtta gttatgtttt ctagcctgtg
      241 ctaaaggaaa ttaagtaaag acagataaat gtagactaaa acgtgccgca tcaaagtgcc
      301 gctttttcaa gttccttaag aatggcttca attctctcta tacactcagt tggaatacga
      361 gatctatcta ggtttaacac cattttatcg cccttgtaca acactgtcgc tccaggagca
      421 aactgatgtc gtgagcttaa gctagttctt gaggcagatg acgttttaag cacagacgtt
      481 aaaagagtga tgacttcttc agcttcaaat atcactcctg cttttttctg ctcgtgaagg
      541 ttagatgcct gctgcttaag taattcttct ttatctgtaa aagctctttg aagtgcatca
      601 cctgaccggg cagatagttc accgggatga gaaaaaagag caacaactga tttaggcagt
      661 ttagctgtgt tgatacagcg ggtaataatc ttacgtgaaa tattttctgc atcagccagc
      721 gcagaaatat ttccagcaaa ttcattctgc aatcggcttg cataacgctg accacgttca
      781 taagcacttg ttggcctata atcgttaccc aatctggaca atgcagccat ctgctcatca
      841 tccagctcac caaccagaac tcgataatca ctttctgtaa gtgcagcagc tttacgacgg
      901 cggctcccat cggcaatttc tatgacccca gacactcttc gaccgaatgc tggtgtctgc
      961 tgacctgtca gtagaaaaga agggataaga tcatccaggg cgtcttcggt aagcagttcc
     1021 tggtcacgtt cattacctga ccatacccga gaggtcttct caacactatc tccccggagc
     1081 acctcaaggg taaatttcac gtccctacca catacaggca aagtaatagc attgccacga
     1141 gccatagctc ctacgcgcgc aattaacgaa tccaccatcg gtgcagctgg tgccgatgac
     1201 ggagtatctt caagcggttg agtcttaatc gtatgttttg gaatgacagg cgcacgcttc
     1261 attatctaat ctcccagcgt ggtttaatca aacgatcgaa aatttcattg cagacaggtt
     1321 cccaaataga aagggcattt ctccaggcac ctgttgaaga gcgttgatca atagcctgtt
     1381 caaaaacagt tctcatccgg atctgacctt taccaacttc atccgtttca cgcacaacat
     1441 tttttaggac catgcttccc caggcgtccc gaatttgctc ctccatccac ggggactgag
     1501 aaccattact attactgtat ttggtaagca aaatacgtac atcaggctcg aatcctttaa
     1561 gatctacgtt tttgagcaga tcacgaagca tatcgaaaaa ctgcagagcg gaagtgtagt
     1621 cgaacaactc agcaggcgtg gggacaatca acacatcagc agcacataca acattaatcg
     1681 tgccgatacc taggttaggc gcgctgtcaa tgacaatgac atcatagtca tgagcgacgg
     1741 tttcaatggc cagtcggagc atcaggtgtg gatcggtggg caatttacct tcatcaaatt
     1801 tgcccattag ctcagtttca atgcggtgca gagccaaaca ggaaggaata atgtcaagcc
     1861 caggccagca agtaggcttt attgcataag tgacatcgtc cttttcccca agatagaagg
     1921 gaaggagagt atcctctgca tgaatatgaa gatctggcac ccagccgtga tacatcgatg
     1981 ctgttccctg ggggtcgtta ccttccacga gcagaacacg taatcccttc agagccagat
     2041 cctgagcaag atgaacagaa acagaggttt tgtaaacgcc ccctttatga gcagcaactc
     2101 caatcaccgg cggaaatacg tcttcagcac gtcgtagtct cgtaccaaac acgtcacgca
     2161 tatgattaat ttgttcaatt gtataaccaa cacgttgctc aacccgtcct cgtatttcca
     2221 tatccgggtg cggtagccgg cctgctttct cggcatccct gatagcctga gaagatactc
     2281 caactaaatc cgctgcttca cctattctcc agcgtcgggt gattttcctc gcttccgggc
     2341 tgtcatcatt aaactgtgca atggcgatag ccttcgtcat ttcatgacca gcgtttatgc
     2401 actggttaag tgtttccatg agtctcattc tgaacatcct ttaatcattg ctttgcatat
     2461 tttcatttaa tcttgcattt tattgcaaag caacaacaaa attgcaaagt agtcaaaaaa
     2521 ccgcaaagtt gtttaaaatg agagcaacac atcaaaaaag gaaacaggaa gagcacatac
     2581 ctcagtcaat aattatcact agtactcgta gccgtcgtgt aaccgagcgt agcgagctaa
     2641 ctggcgagga cgcgcgctaa ctgttcacac tcagtatccc tagcaccaaa ataactatct
     2701 gatggtcaag gcggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2761 gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg
     2821 tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct
     2881 gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca
     2941 tatcaccacg tcaaagggtg acagcaggct ca
//
LOCUS       DACAPB010000303         2949 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.309, whole genome shotgun sequence.
ACCESSION   DACAPB010000303 DACAPB010000000
VERSION     DACAPB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.309"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            270..1718
                     /locus_tag="IT904_004590"
     CDS             270..1718
                     /locus_tag="IT904_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP3194258.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1720..1845
                     /locus_tag="IT904_004591"
     CDS             1720..1845
                     /locus_tag="IT904_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194259.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1842..1913)
                     /locus_tag="IT904_004592"
     CDS             complement(1842..1913)
                     /locus_tag="IT904_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194260.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            1968..2516
                     /gene="idi"
                     /locus_tag="IT904_004593"
     CDS             1968..2516
                     /gene="idi"
                     /locus_tag="IT904_004593"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP3194261.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2559..>2949)
                     /gene="lysS"
                     /locus_tag="IT904_004594"
     CDS             complement(2559..>2949)
                     /gene="lysS"
                     /locus_tag="IT904_004594"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="class II; LysRS2; catalyzes a two-step reaction,
                     first charging a lysine molecule by linking its carboxyl
                     group to the alpha-phosphate of ATP, followed by transfer
                     of the aminoacyl-adenylate to its tRNA; in Methanosarcina
                     barkeri, LysRS2 charges both tRNA molecules for lysine
                     that exist in this organism and in addition can charge the
                     tRNAPyl with lysine in the presence of LysRS1; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP3194262.1"
                     /translation="EEVAEAHLIQPTFITEYPAEVSPLARRNDINPEITDRFEFFIGG
                     REIGNGFSELNDAEDQAQRFLDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIG
                     IDRMVMLFTNSHTIRDVILFPAMRPVK"
BASE COUNT          677 a          694 c          725 g          853 t
ORIGIN      
        1 agcgctatcc agataaaaac gattaaatgc gagagtgcga catgccagaa tgattaatga
       61 aatatcaata tgagaaaatt acagataatt ccattgtcag gttctcttgt cattttcgtg
      121 gtttaagcac gattatgtga ttgcctccgc aattcctgtc tctaactccc cttcctcgca
      181 aaaactggca ctccacgagc atgtgtttag acagtttcat taacgtaaac ggttgctttt
      241 tactctggcg ggcgaaagga gaaacactga tgagcgccat agattcccaa cttccctcat
      301 cttctgggca agaccgccca actgatgagg ttgaccgcat attatcacca ggaaagctga
      361 tcatactcgg tctgcaacac gtccttgtca tgtacgcagg tgcagtcgct gttcctctta
      421 tgattggtga ccgactgggc ctctcaaaag aagctattgc gatgctcatt agctcggatc
      481 tcttttgctg cgggatcgtc acattattgc aatgtatcgg tatcggccgc tttatgggga
      541 tccgcctgcc ggtgattatg tcggtgacct ttgctgctgt aacaccaatg atagccattg
      601 ggatgaaccc ggatatcggc ctgctgggga tatttggtgc cactatcgcc gcgggtttta
      661 tcaccacatt attagcgcca cttatcggtc gcttgatgcc tttattcccg ccactggtta
      721 ccggtgtggt tattacttct atcgggctta gcatcattca ggtgggtatt gactgggccg
      781 ccggaggtaa agggaatccg caatatggta atcccgttta tttaggtatc tcctttgccg
      841 tcttaatttt tatcttgctc attactcgct atgcgaaagg atttatgtcc aacgtcgccg
      901 tattactggg gattgtattt ggctttttac tttcgtggat gatgaatgaa gtcaatttat
      961 ccgggctaca tgatgcttca tggtttgcga ttgttacgcc gatgtcgttt ggtatgccga
     1021 ttttcgatcc cgtttccatt ctgaccatga ctgccgtgtt aatcatcgtg tttatcgagt
     1081 cgatggggat gttcctggca ctgggtgaaa tagtcggtcg taaactctct tcgcacgata
     1141 ttattcgcgg gctgcgtgtc gatggcgtag ggacaatgat aggcggcacg tttaacagct
     1201 tcccccacac gtcattttca caaaacgttg gcctggttag cgtgacgcgc gttcatagcc
     1261 gctgggtgtg tatttcttcg ggaattatat taatcctgtt tggcatggtg ccaaaaatgg
     1321 cggtgctggt cgcctccatt ccgcaatttg tgctgggcgg tgctgggctg gtgatgttcg
     1381 gcatggtact ggcgacaggg attcgaattc tgtcgcgctg taactacacc accaaccgtt
     1441 acaacctcta tattgtggcg atcagtctcg gcgttggcat gactccgacg ctctctcacg
     1501 atttcttttc taagttaccg gccgtactgc aaccgttgct gcatagcggc attatgctcg
     1561 caacccttag cgccgttgtg ctgaatgtct tctttaatgg ctaccagcat catgctgacc
     1621 tggtgaagga atccgtctct gataaagatt taaaagtcag gacagtacgt atgtggcttc
     1681 tgatgcgcaa gctgaagaaa aatgagcatg gagaataata tgaatttttt aatgcgcgct
     1741 atattcagtc tgctgttgct ttttactctc tctattcctg tcatttctga ctgtgttgca
     1801 atggccattg aaagtcgctt caaatatatg atgctacttt tttaaatggt ttttacctgt
     1861 cggcatccgc tcaaaacggg cggttgtcga taaacgctca cttggttaat catttcactc
     1921 ttcaattatc tataatgatg agtgatcaga attacatgtg agaaattatg caaacggaac
     1981 acgtcatttt attgaatgca cagggagttc ccacgggtac gctggaaaag tatgccgcac
     2041 acacggcaga cacccgctta catctcgcgt tctccagttg gctgtttaat gccaaaggac
     2101 aattattagt tacccgccgc gcactgagca aaaaagcgtg gcctggcgtg tggactaact
     2161 cggtttgtgg gcacccacaa ctgggagaaa gcaacgaaga cgcggtgatc cgccgttgcc
     2221 gttatgagct tggcgtggaa attacgcctc ctgaatctat ctatcctgac tttcgctatc
     2281 gcgccaccga tccgaatggc attgtggaaa atgaagtgtg tccggtattt gccgcacgca
     2341 ccaccagtgc gttacagatc aacgatgatg aagtgatgga ttatcaatgg tgtgatttag
     2401 cagatgtatt acacggtatt gatgccacac cgtgggcgtt cagtccgtgg atggtgatgc
     2461 aggcagccaa tagtgaagca agaaaattgt tgtctgcttt cgcgcagcac aattaaaaaa
     2521 ccccgacatt tgccggggtt gtgagcataa cgtaatgctt attttaccgg acgcatcgcc
     2581 gggaacagaa taacgtcgcg gatggtatgg ctgttggtga acagcattac catacggtcg
     2641 atgccaatcc ccagacctgc tgtcggcggt aagccatgtt ccagtgcggt aacgtaatct
     2701 tcatcgtaga acatcgcttc gtcgtcacct gcgtctttcg cggcaacctg atccaggaag
     2761 cgttgcgcct gatcttccgc gtcattcagc tcgctaaagc cgttaccgat ttcacgacca
     2821 ccgatgaaga actcaaagcg gtctgtgatt tccgggttaa tgtcgttacg acgcgccagc
     2881 ggagaaactt ctgccggata ttcagtaatg aaggtcggct gaatcagatg tgcttctgcc
     2941 acttcttcg
//
LOCUS       DACAPB010000304         2888 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.310, whole genome shotgun sequence.
ACCESSION   DACAPB010000304 DACAPB010000000
VERSION     DACAPB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.310"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            236..1495
                     /gene="codB"
                     /locus_tag="IT904_004595"
     CDS             236..1495
                     /gene="codB"
                     /locus_tag="IT904_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAP3194263.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1485..2768
                     /locus_tag="IT904_004596"
     CDS             1485..2768
                     /locus_tag="IT904_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HAP3194264.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
BASE COUNT          617 a          731 c          805 g          735 t
ORIGIN      
        1 gtaggtcgga taaggcgctc gcgccgcatc cgacaccatg ctcagatgcc tgatgcgacg
       61 ctgacgcgtc ttatcaggcc tacccactgt ttttacaccg ataatttttc ccccaccttt
      121 ttgcactcat tcatataaaa aatatatttc cccacgaaaa cgattgcttt ttatcttcag
      181 atgaatagaa tgcggcggat tttttgggtt tcaaacagca aaaaggggga atttcgtgtc
      241 gcaagataac aactttagcc aggggccagt cccgcagtcg gcgcggaaag gggtattggc
      301 attgacgttc gtcatgctgg gattaacctt cttttccgcc agtatgtgga ccggcggcac
      361 tctcggaacc ggtcttagct atcatgattt cttcctcgca gttctcatcg gtaatcttct
      421 cctcggtatt tacacttcat ttctcggtta cattggcgca aaaaccggcc tgaccactca
      481 tcttcttgct cgcttctcgt ttggtgttaa aggctcatgg ctgccttcac tgctactggg
      541 cggaactcag gttggctggt ttggcgtcgg tgtggcgatg tttgccattc cggtgggtaa
      601 ggcaaccggg ctggatatta atttgctgat tgccgtttcc ggtttactga tgaccgtcac
      661 cgtctttttt ggcatttcgg cgctgacggt tctttcggtg attgcggttc cggctatcgc
      721 ctgcctgggc ggttattccg tgtggctggc tgttaacggc atgggcggcc tggacgcatt
      781 aaaagcggtc gttcccgcac aaccgttaga tttcaatgtc gcgctggcgc tggttgtggg
      841 gtcatttatc agtgcgggta cgctcaccgc tgactttgtc cggtttggtc gcaatgccaa
      901 actggcggtg ctggtggcga tggtggcctt tttcctcggc aactcgttga tgtttatttt
      961 cggtgcagcg ggcgctgcgg cactgggcat ggcggatatc tctgatgtga tgattgctca
     1021 gggcctgctg ctgcctgcga ttgtggtgct ggggctgaat atctggacca ccaacgataa
     1081 cgcactctat gcgtcgggtt taggtttcgc caacattacc gggatgtcga gcaaaaccct
     1141 ttcggtaatc aacggtatta tcggtacggt ctgcgcatta tggctgtata acaattttgt
     1201 cggctggttg accttccttt cggcagctat tcctccagtg ggtggcgtga tcatcgccga
     1261 ctatctgatg aaccgtcgcc gctatgagca ctttgcgacc acgcgtatga tgagtgtcaa
     1321 ttgggtggcg attctggcgg tcgccttggg gattgctgca ggccactggt taccgggaat
     1381 tgttccggtc aacgcggtat taggtggcgc gctgagctat ctgatcctta acccgatttt
     1441 gaatcgtaaa acgacagcag caatgacgca tgtggaggct aacagtgtcg aataacgctt
     1501 tacaaacaat tattaacgcc cggttaccag gcgaagaggg gctgtggcag attcatctgc
     1561 aggacggaaa aatcagcgcc attgatgcgc aatccggcgt gatgcccata actgaaaaca
     1621 gcctggatgc cgaacaaggt ttagttatac cgccgtttgt ggagccacat attcacctgg
     1681 acaccacgca aaccgccgga caaccgaact ggaatcagtc cggcacgctg tttgaaggca
     1741 ttgaacgctg ggccgagcgc aaagcgttat taacccatga cgatgtgaaa caacgcgcat
     1801 ggcaaacgct gaaatggcag attgccaacg gcattcagca tgtgcgtacc catgtcgatg
     1861 tttcggatgc aacgctaact gcgctgaaag caatgctgga agtgaagcag gaagtcgcgc
     1921 cgtggattga tctgcaaatc gtcgccttcc cgcaggaagg gattttgtcg tatcccaacg
     1981 gtgaagcgtt gctggaagag gcgttacgct taggggcaga tgtagtgggg gcgattccgc
     2041 attttgaatt tacccgtgaa tacggcgtgg agtcgctgca taaaaccttc gccctggcgc
     2101 aaaaatacga ccgtctgatc gacgttcact gtgatgagat cgatgacgag cagtcgcgct
     2161 ttgtcgaaac tgttgctgcc ctggcgcacc gtgaaggcat gggcgcgcga gtcaccgcca
     2221 gccacaccac ggcaatgcac tcctataacg gggcgtatac ctcacgcctg ttccgcttgc
     2281 tgaaaatgtc cggtattaac tttgtcgcca acccgctggt caatattcat ctgcaaggac
     2341 gtttcgatac gtatccaaaa cgtcgcggca tcacgcgcgt taaagagatg ctggagtccg
     2401 gcattaacgt ctgctttggt cacgatgatg tcttcgatcc gtggtatccg ctgggaacgg
     2461 cgaatatgct gcaagtgctg catatggggc tgcatgtttg ccagttgatg ggctacgggc
     2521 agattaacga tggcctgaat ttaatcaccc accacagcgc aaggacgttg aatttgcagg
     2581 attacggcat tgccgccgga aacagcgcca acctgattat cctgccggct gaaaatgggt
     2641 ttgatgcgct gcgccgtcag gttccggtac gttattcggt acgtggcggc aaggtgattg
     2701 ccagcacaca accggcacaa accaccgtat atctggagca gccagaagcc atcgattaca
     2761 aacgttgaac gactgggtta cagcgagctt agtttatgcc ggatgcggcg tgaacgcctt
     2821 atccggccta cgtagagcac tgaactcgta ggcctgataa gcgtagcgca tcaggcaatt
     2881 ccagccgc
//
LOCUS       DACAPB010000305         2874 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.311, whole genome shotgun sequence.
ACCESSION   DACAPB010000305 DACAPB010000000
VERSION     DACAPB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.311"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(51..953)
                     /gene="fimH"
                     /locus_tag="IT904_004597"
     CDS             complement(51..953)
                     /gene="fimH"
                     /locus_tag="IT904_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAP3194265.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(973..1476)
                     /gene="fimG"
                     /locus_tag="IT904_004598"
     CDS             complement(973..1476)
                     /gene="fimG"
                     /locus_tag="IT904_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAP3194266.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGTTQGTIQAVI
                     SITYTYS"
     gene            complement(1489..2019)
                     /gene="fimF"
                     /locus_tag="IT904_004599"
     CDS             complement(1489..2019)
                     /gene="fimF"
                     /locus_tag="IT904_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAP3194267.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(2029..>2874)
                     /gene="fimD"
                     /locus_tag="IT904_004600"
     CDS             complement(2029..>2874)
                     /gene="fimD"
                     /locus_tag="IT904_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAP3194268.1"
                     /translation="VNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTL
                     LEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSG
                     GVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENR
                     VALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMV
                     TSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQL
                     SAECR"
BASE COUNT          699 a          781 c          681 g          713 t
ORIGIN      
        1 tagcttcagg taatattgcg tacctgcatt agcaatgccc tgtgatttct ttattgataa
       61 acaaaagtca cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt
      121 gcgtaatttg ccgttaatcc cagacttacc gccgaagtcc ctactgctcc taacgatacc
      181 gtgttattcg ctggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct
      241 ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag
      301 aggtaatacc ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa
      361 ccagggtagt ccggcagagt aacggtgaca tcacgagcag aaacatcgca gccgccagta
      421 ggcaccacca catcattatt ggcgtaaata ttccacacaa actggaaatc atcgctgtta
      481 tagttgttgg tctgtcgcaa aataagcacg gcaattaatg agccagcttt aatcgccacc
      541 ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc
      601 gaattataaa caacccgcgg cgtttcgctg gtggtcggaa atggatagct actgccacta
      661 tattttacgg tcccggaaaa attagataac acgccgccat aagccgagcc tcgttgcagt
      721 gtgacatagt ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga
      781 tctacgacca ggttttgccc cacattcacg acaggcgcaa ggtttacata aacattagcg
      841 ctgccaccac caatagggat agcggtacca ttggcggttt tacaggcgaa tgaccaggca
      901 tttaccgacc agcccatcag cagtacagca aacagggtaa taactcgttt cattacaatc
      961 atctcttcgg gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg
     1021 agtggttccg ccatttaccg ttaatgctct gacctgtaac gggaagtgcg ctgattgtga
     1081 ggaatcatcc acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc
     1141 ctgtagctct aactggatgt tttgcgcggt cccctggttt ttataatatc cggtactgtc
     1201 ggctgccccg ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc
     1261 aagcgcaaca tcatgccagg ccgatgccgc cccggcagac atcagactga aagaataaag
     1321 atcgccgaga tcaaccgtgg cattggtggt ggaaactgtg cacggtttgg cgacgacctt
     1381 accgttcacc gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaatat
     1441 tgccgccaat acatacccac gtttgcacca tttcatgagc atctccagtt actgatattc
     1501 aagagtgaag gtagccgtgg cattgatatg ccccgcagtg acaggcacct gtgtcgccat
     1561 tagccgggcg taaaaattca gcgtatttgg tttacccggc gtcagggtcg tccacgaaat
     1621 cgcggacgat ggagcattaa ggggtatctg attttgctgc tcattcagaa gctgtattcc
     1681 cagtcccgaa gccgctgaca ccgtattttc aagtgcaagc aggttggcat tgtggctatc
     1741 tgcaacgccg gtaaacccaa cttttacggc agaaacggca ttaccacagg gtgacagcaa
     1801 aatacgaaat ggaacgacag gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt
     1861 ttccatcaga tcaacagtaa aattggttga ttcagcggcc acactacagc cgttatctct
     1921 gacatagccg cggatagtaa tcgtgctatc cgcagccaaa gcgtgactca ccgccagcca
     1981 caaaaaagcg cacagaagat aaaaaggttt gtttctcatc acgccccctt aacgacattc
     2041 agctgatagc tgggttaata actgctgctg actctctggt ggcagttgat aattggcgac
     2101 acagtgagca ttttcctctt ctccccattt cacctgaact tttcccgcta aaggcattcc
     2161 gctgaggtaa acctgaccat tatccgcaac aatgccgcta ctctggctac tctctgatgt
     2221 caccatcgcc ccgaacggca gcggcttatt attgtgggtt agcgtcatga gcagttttat
     2281 cccaacgcgc gctttaaact ctgctcgcac gatcgcccca cgagtgggaa caacgttagc
     2341 gaccgcgtta tctaaatcga cgttatcagc cagggtattg gtatccagcg ccactctatt
     2401 ttcccgatat tcagtggcat aaggcagcac ggcataaccg cgccagtcgg tacgcacccc
     2461 cgtctggttt tcgacttttg catcttttgc gccaggcgct ttaacaagca ccaccgtatc
     2521 gtttaacggc tgccccagcg ttacgccatt ggcatgagcc agtaccccac cgctgactcc
     2581 gtaatagagc tgcttaatat catcgctatg gctgtaaccg atattggcat tgccgtaacc
     2641 accgcgataa ttcagcgtgg cgtagcctgt acttccgcta ttaccatcgc ctcccccggc
     2701 atagccggtt tgcacgctat agctgaggtt gttgtcttcc agcaacgtac cgtatacacc
     2761 agccagattg gtcattcgac cgttgagatc gtgtgacatg ctgtagctgg cactggcatg
     2821 tcgccactga gatttactgt cagaacgcag ccagtggctg aaaggaatat tgac
//
LOCUS       DACAPB010000306         2816 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.312, whole genome shotgun sequence.
ACCESSION   DACAPB010000306 DACAPB010000000
VERSION     DACAPB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.312"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..118)
                     /locus_tag="IT904_004601"
     CDS             complement(<1..118)
                     /locus_tag="IT904_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP3194269.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAK"
     gene            complement(212..403)
                     /locus_tag="IT904_004602"
     CDS             complement(212..403)
                     /locus_tag="IT904_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAP3194270.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(400..588)
                     /gene="dicB"
                     /locus_tag="IT904_004603"
                     /gene_synonym="ftsT"
     CDS             complement(400..588)
                     /gene="dicB"
                     /locus_tag="IT904_004603"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HAP3194271.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEEAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            989..1153
                     /locus_tag="IT904_004604"
     CDS             989..1153
                     /locus_tag="IT904_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194272.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHSTSPVEN
                     SLITNLSFVG"
     gene            complement(1157..1375)
                     /locus_tag="IT904_004605"
     CDS             complement(1157..1375)
                     /locus_tag="IT904_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194273.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            1468..1668
                     /locus_tag="IT904_004606"
     CDS             1468..1668
                     /locus_tag="IT904_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194274.1"
                     /translation="MNTIKFSCPECGGEVFDTSFKPQGSDSFAGAICKSCGHLVTEDE
                     SSQFDDEIVDNIFGALTRDFLK"
     gene            complement(2105..2524)
                     /locus_tag="IT904_004607"
     CDS             complement(2105..2524)
                     /locus_tag="IT904_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3194275.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            2604..>2816
                     /locus_tag="IT904_004608"
     CDS             2604..>2816
                     /locus_tag="IT904_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP3194276.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGR"
BASE COUNT          693 a          663 c          618 g          842 t
ORIGIN      
        1 attttgctcg agcacggcgt tcgtcgccag cttcaatggc tgtggctaca gccacagcgc
       61 cttcttccct tgttgccagt tcgtcaggaa tagcggcgca aataaagact ttactcattt
      121 tgttttaacc tcatgacaga tttaaggatg aacaaatccc tgccattgct ggcatataag
      181 aatgaaaccg gatatttatt acggaactgt tttaaagacc tgccgggatt tcgatattat
      241 cctggtgaat aactttatcg accgggtaac agttaccggg aattttctgt tcggttgctg
      301 cagtcataca ctcctgcatt gtcctgtgaa cactgactgc aatatcaact ggctctccgg
      361 aaacaagaaa aactgtcaga acaagcgcaa atgctgaatt cattgtgcac atccttttgg
      421 catcagacgt aaacgagcca gcattgaaac aatgcatatt ttatttaata gctcccgttc
      481 ttgttttctc ttgttaatgg cctcttcagt aaatacaggg ttactgatag tgacaccaat
      541 ttcaaaacaa ccttcagacg tattaacgtt tggtaataac gttttcatta tcgcgccctc
      601 aacaatgagt tttgtgatgc ggtgcctggt gcctccaggt gacgttaacc agttaacaat
      661 taacgccgga tacagagaat ccacccataa cactgttttt ggttttaact gttccgcgtg
      721 cgcttagccg cattcaccgc atcacaaaat tcactttaaa aagggcggca gagcagtcac
      781 ggagtaaaac tgataccgcc aaatgtcacc agaaaattga taacagaggg cgttgcagcg
      841 gggttgtcac ttaagcgtat ggtcaacctg acaacccggt gtcctcaacg ggggaaggaa
      901 taaccccgcc atacttaccg ccgcgccatt tcgcggattg ccacaaccgg aagcgcacgg
      961 tcgacgaaaa tttaacgaca ggctatctat gaaccagcta cctcgccgtg cgctttcgcg
     1021 ttatggtctg acttttcagg gaaatatcct ttcagtaaac tgtcagtgcc ggatgctcac
     1081 ccgtgtccgg cgcacgcact ccacctcacc cgtggagaac tccttaatta ccaaccttag
     1141 cttcgttggt tagctattaa cgcgggtatg taatcattct ggcaatgctt aatgccgctg
     1201 ctttttccag cctggtgata tcctgctcca gagcggacag attttcagcc tgcttagccc
     1261 tggcttcatt ggcccatttc aaatcctgcg ctgcattaat tttctggcgc atccactcat
     1321 aaagttcatc atcggtatag tctggcgcga tgatgacggg ttctcgtttc tgcatgtcgg
     1381 ctccttgtgg ttagcgttgc ctgcttttaa ccacgtcagg cgaggtggta tcctctgagg
     1441 ggtctgttac tcgagaggaa attggttatg aatacaatca agttttcttg cccagaatgt
     1501 ggtggcgaag tctttgacac atcctttaaa ccgcagggct ctgacagttt cgcgggagcc
     1561 atctgcaaaa gttgtggtca ccttgtaact gaagatgagt cctcgcagtt tgatgacgaa
     1621 atcgttgaca atatcttcgg tgcactcacc agagactttc tgaagtaaag gcgcataccg
     1681 cttagttacc gctctgataa ttcttacctg tccggcaatg gcgctgatat caatataaag
     1741 cgccatcgct gtttctttgc tgatccctgg acgccttcca ttctgatgtt tgacttcgcc
     1801 cactgagaaa tcctctgttt ccccttaacg ccggggtagc ggaacaaaaa cctgctgcat
     1861 agttattaaa gttgaaccct gccgtcatgt tcttacgcct cgggctggct acttaacccc
     1921 tgaccactgc ctggtaactc gaagtattgc cctgcattct gtggggcggg gtgggtggca
     1981 ggcatataat gtacttttcg ttcattgttg taaagtactt ttagtacatt ttgtgtgtaa
     2041 aaaaatgaga tgggataaag tgaagcacaa acccggagga aggcgctacc ggatttatgc
     2101 tggtttaaga ggctttttgt tttttctttc gtgctaactc ttcgtaaatt gcattgtact
     2161 tctgtttttt ctcttcaaga gtttttaaaa gttcatctgt ctcactgtca gggagctcgt
     2221 ccagaaggtc aatgatgatt ttttgtcttg gatttaactc ctgatagaaa cgtacctgtc
     2281 cactttcttc tgtatcctct cccaaaagat aggttggtgt tgttcctatt agtgttgcta
     2341 attcccttaa tttctcccgg cgaggaattg tttcgccatt aaaccatttg ctaaccgctt
     2401 ttggtgttaa tttcattcga cgggcaattt ctgcctgcct tccatgttgt tcataaccag
     2461 cgttttcaca ggctagcgca agcctactgg cgaactcttt acgcgcttta tcttcatgaa
     2521 ccataagttc aatgatattc gctcttgaat gtactgtcag ttctgttata gcatgtactc
     2581 aaagttcaca ttgtgagggt gatatgaacc agaaaacact tgaagatgta atcaaaactg
     2641 ttcgcgttgc tgttgtggcc gacgtttgtg gtgtcagcca aagagcaatc tataaatgga
     2701 tggataacgg aaaattgcct cgcacagaat ataccggcga aacaaattac gctgaaaaaa
     2761 tcgctcttgc atcaaacgga ttattttctg ccgatgcaat tttaactatt ggcagg
//
LOCUS       DACAPB010000307         2809 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.313, whole genome shotgun sequence.
ACCESSION   DACAPB010000307 DACAPB010000000
VERSION     DACAPB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.313"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(10..414)
                     /locus_tag="IT904_004609"
                     /pseudo
     CDS             complement(10..414)
                     /locus_tag="IT904_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
     gene            complement(538..735)
                     /locus_tag="IT904_004610"
                     /pseudo
     CDS             complement(538..735)
                     /locus_tag="IT904_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405579.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(763..2082)
                     /gene="potE"
                     /locus_tag="IT904_004611"
     CDS             complement(763..2082)
                     /gene="potE"
                     /locus_tag="IT904_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAP3194277.1"
                     /translation="MSQAKSNKMGVVQLTILTMINMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(2079..>2809)
                     /locus_tag="IT904_004612"
     CDS             complement(2079..>2809)
                     /locus_tag="IT904_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAP3194278.1"
                     /translation="VDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKC
                     DLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTL
                     RQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEG
                     RIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDG
                     RKQVWCYVIKPRDAQSTLLKGEKL"
BASE COUNT          751 a          735 c          643 g          680 t
ORIGIN      
        1 cagactttac caattcagcg tatctggcgc aaaataataa acgcagccag tatcttcccc
       61 aagatcgcca ccattaagag caaaaattcc ccctgcgaca tcgtcagcga tgggtaaagc
      121 ccgcacgcct gcgaattcag ggcgttgtgt ccaacttact ggatctcgtg gcaaccgggg
      181 gtgaccagat cctgctatgc gtaaccaacc attatcaatc agcagacctc cggagccata
      241 gacaagcgca cccattggtg attttgttgt cacctgtaat tgataaagtg cggtttcggc
      301 taaatcttta ttaaccggta agagttcggt atggtttgtg gcgtctttta accagttctg
      361 gattattggc caggctgaat cttcagtatt tactaactga ttcatttcac gcatcgctac
      421 ttgctccttg tgtgctacgg tgtcatttat gatgaggaat tgtatggaac attttttaaa
      481 tattaagttc cttatatttg aataaaaaat gactttatcc atacattgcc acttttttca
      541 aagcataaac cgatacccaa taccggtttc agtaataaaa tggcgaggcc gggccggatc
      601 ctgttccagt ttttgtcgca gatgccccat ataattacgc aaatagttgc tgtgttcgat
      661 cgcgtttaga ccctacatct gccggatgcg gcataaataa cgaactattt gccaacaaac
      721 agaaagggcg atcattcaat cgcccttttt tactttgttt tattaaccgt gtttattttt
      781 cagttcaaag cgtggtgata ccagaccata cagtgtccaa ccaaggaagg tcacgatgga
      841 accgtacagc atggcttctt ccccggatga gtacagcgca tagaaactgt acatcgcgcc
      901 aacaaaagca acaaagtttg caactttcgc ttttgagggg ggcacattag cgaccttctg
      961 aataatgact aatgccgcca ttgacagaat atacggaatg atattggtca ccacggccag
     1021 gttaaccagc acgttgaact gactgtttag cgacgggcta atcgtcatca gcgccaaccc
     1081 gctctggata atcacaatgg tcaacattcc ctgcaccggt gcatccactt tggttacacg
     1141 ggagaaaatt ttagggaagt agccttcatc agatgaagat ttaaacacct gggcaatggt
     1201 gaactgccag ccaagtagcg aaccgcagca ggacatcacc atcagcgcca taatgacttt
     1261 acccacttcc ggcgtgaaca tctgcgcgaa ggccagacca aatggtgccg ttgaatttgc
     1321 cagctccata tttggcacaa tcccggcaat cacgttggtg gagacgatat aaatcaccgc
     1381 cgcacctaac gtaccgccga gtaccgcgat tggcacatta cgttccgggt tttccactac
     1441 atcagtattt gcacacgcag actccagacc aagaaaagcc cacagcgtca tagcgatgga
     1501 agaacctacc gcactgaaga acggtgcatg atgcggattc caggaatcaa cgtacagcgt
     1561 cgggctaaac cagaaccagc caataatgca cagaccgacg accggaataa tgacccccca
     1621 cacggtaatg ctactgattt gcccggtaat gcgcgcacca ccaaagttag ccacggtaca
     1681 aatccacagc accccgatgg tcgcaagacc aatctgcact ggcgacaaac tcgcgccgag
     1741 cagttcggtg ccgtaaccaa ccgccgaaat agcaatcgcg acgttagcaa tcagcagtga
     1801 gacgccgtag gtatagttcg ccataaagtt accggattta ccgaaagcat actcggcata
     1861 accgcccata ccgcctgatt tacggctgaa cataccgcat tttgcgaatg cccatgccag
     1921 tgccattgag cctacggctg tcaccagcca ggagataatt gagattgtcc cgacttcggc
     1981 aagctttgtc ggcagcatga tgataccgga gcccatcatg ttgatcatcg tcagtatggt
     2041 taactgaacg acgcccattt tgttcgattt agcctgactc ataatttttc ccctttcaac
     2101 agggtgcttt gcgcatcacg aggcttgatg acatagcacc aaacttgctt acgaccatca
     2161 tgttcttcga tatagacacc ctgcagctcc ggtgcaaaac ctggcagcag gttgatccct
     2221 tcttccagag cgctgaagta acgcagaaca gcaccacccc agatttcacc cggaacaaca
     2281 cacagcacac ccggaggata aggaagcgca ccttcggcag cgatacggcc ttctgcatcc
     2341 ggcagacgaa ccagttccac ttcaccgcgt aaataggcgt agttggcttc ttgcggattc
     2401 atgctgacgc gtgggaagtg ctccttacgg aacatctctt tttgcagttg tttcacgttg
     2461 tggcgggcat acaaatcatg catttcctga cacaactgac gcagggtata accggcgtag
     2521 cgctcttcat gctgtttgta gatggaaggt agcacttctg ctaatggcgc gtcggactca
     2581 agcagttttt cgaagcgtac cagcagggca acaagttgct gaagtttggc catatcttct
     2641 gccggggtca gcaggaagag gatggagtta agatcgcatt tttccggcac tacgccattt
     2701 tcgcgtagga agttagcaag aatcgtcgcg ggtacaccga acgcttcata ttcgccgtta
     2761 cgtgcatcaa tacctggggt tgtcagcaac agtttgcatg gatcgacaa
//
LOCUS       DACAPB010000308         2757 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.314, whole genome shotgun sequence.
ACCESSION   DACAPB010000308 DACAPB010000000
VERSION     DACAPB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.314"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..2658
                     /locus_tag="IT904_004613"
     CDS             <1..2658
                     /locus_tag="IT904_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP3194279.1"
                     /translation="YSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWSDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADST
                     IASWDDVALAVVAHPNADNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDAD
                     GEVDTNGWDGTDRLDLTLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATA
                     TLIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTGSQWDTTKTS
                     LIDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDE
                     VSAGWAVGDAALYANNIKVTNDGILDVGNTASNALQVDTLNLTSTTDTSGNIHAGVFN
                     IESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNE
                     VDNNSDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGA
                     YTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADN
                     GGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMND
                     RSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAW
                     GFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDA
                     GYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKN
                     FSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT          741 a          636 c          658 g          722 t
ORIGIN      
        1 tattccgagt gcatgacaac tgattgtgct gaacgtgcga atgattatta ccatgaccgt
       61 ctggcgctga ctgttgataa ttcaacgatc gacgacaact acgaacacta cacatataac
      121 ggcacctaca ataatgccgc tgacactcat gttgtaaatg tatttaacat cggtactgcc
      181 atcactctgg atcaggaagt tgatctgtcc atctctaata actctcacgt agcgggtatt
      241 acgctgactc agggttacga gtgggaagat atcgacgaca acacggttag cactggtgtt
      301 aacagcagcg aagtgtttaa caacaccatc actgttaaag actctactgt gacctctggt
      361 tcatggtctg atgaaggtac tactggttgg ttcggtaata caggtaatgc cagcgattat
      421 agtggtaaat ctaactttgt caccgtagat actgacggag atggtgttgc tgatagcact
      481 attgcaagct gggatgatgt tgcattagct gtagttgcac atccgaatgc tgataatgct
      541 atgcagacca ccgctgactt tagtaactct actttgatgg gcgatgtaat cttctctagc
      601 aactttgatg aaaacttctt cccacgtggt gcagatagtt atcgcgatgc tgatggtgaa
      661 gtagacacca acggttggga tggaacagat cgcctggatc tgacattaaa taacggtagt
      721 aaatgggtag gggcagcaca atctgttcat caaactggtt ctattgatgt tgatggagat
      781 ggtaaaggtg acatcgctac atacggtgtt ggcaccgagg caactgcaac tctgattgat
      841 attgaggata atagcctgtg gccgttatca accgttggtg ttgaaaacga tgatacaagt
      901 tacagtgaat tcgatcatat tactggtaat caggtttacc agagcggtct gttcaatgtg
      961 accctgaata ccggttcaca gtgggatacc accaaaactt ctctgattga tactctgagc
     1021 atcaacagtg gttcaactgt taatgttgct gattcaacgc tgatttctga ctctatctct
     1081 ctgacaggtc tttctgcgct gaacatcaac gaagatggtc atgttgcaac tgattcactg
     1141 actgtcgaca acagtaccgt aaccatttct gatgaagttt ctgctggttg ggctgtaggt
     1201 gatgctgctc tgtatgccaa caacatcaaa gtgactaacg acggtattct ggatgtaggt
     1261 aacactgcgt cgaatgctct gcaggttgat actctgaacc tgaccagcac tactgatacc
     1321 agtggtaaca ttcacgctgg tgtgttcaac atcgaaagca accgcttcgt acttgatgca
     1381 gacctgacca acgaccgtac caacgatact accaagtcaa actacggtta tggcttaatc
     1441 gcaatgaact ctgatggtca cctgaccatt aacggtaacg gcgataacga caacactgct
     1501 tctatcgaag ctggtcagaa cgaagttgat aacaacagtg accatgttgc agctgcaacc
     1561 ggtaactaca aagttcgtat cgacaacgct actggtgctg gttctatcgc tgactacaac
     1621 ggcaacgagc tgatctacgt caacgacaaa aatagcaacg cgaccttctc tgctgctaac
     1681 aaagctgacc tgggtgcata cacctatcag gctgaacagc gcggtaacac cgttgttctg
     1741 caacagatgg agctgaccga ctacgctaac atggcgctga gcatcccttc tgcgaacacc
     1801 aatatctgga acctggaaca agacaccgtt ggtactcgtc tgaccaactc tcgtcatggc
     1861 ctggctgata acggcggcgc atgggtaagc tacttcggtg gtaacttcaa cggcgacaac
     1921 ggcaccatca actatgatca ggatgttaac ggcatcatgg tcggtgttga taccaaaatt
     1981 gacggtaaca acgctaagtg gatcgtcggt gcggctgcag gcttcgctaa aggtgacatg
     2041 aatgaccgtt ctggtcaggt agatcaagac agccagactg cctacatcta ctcttctgct
     2101 cacttcgcga acaacgtctt tgttgatggt agcttgagct actctcactt caacaacgac
     2161 ctgtctgcaa ccatgagcaa cggtacttac gttgacggta gcaccaactc cgacgcttgg
     2221 ggcttcggct tgaaagccgg ttacgacttc aaactgggtg atgctggtta tgtgactcct
     2281 tacggcagca tttctggtct gttccagtct ggtgatgact accagctgag caacgacatg
     2341 aaagttgacg gtcagtctta cgacagcatg cgttatgaac tgggtgtaga tgcaggttat
     2401 accttcacct acagcgaaga ccaggctctg actccgtact tcaaactggc ttacgtctac
     2461 gacgactcta acaacgataa cgatgtgaac ggtgattcca tcgataacgg tactgaaggg
     2521 tctgcggtac gtgttggtct gggtactcag ttcagcttca ccaagaactt cagcgcctat
     2581 accgatgcta actacctcgg tggtggtgac gtagatcaag attggtccgc gaacgtgggt
     2641 gttaaatata cctggtaata ttcttcactc cgaagaaatt ctggtaattt aatctaaata
     2701 atgcccgtca aggatttgac gggcattact gcaaaggacg cgcaaatgtt atctgta
//
LOCUS       DACAPB010000309         2742 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.315, whole genome shotgun sequence.
ACCESSION   DACAPB010000309 DACAPB010000000
VERSION     DACAPB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.315"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..203)
                     /locus_tag="IT904_004614"
     CDS             complement(<1..203)
                     /locus_tag="IT904_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP3194280.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEV"
     gene            complement(264..1079)
                     /gene="sul2"
                     /locus_tag="IT904_004615"
     CDS             complement(264..1079)
                     /gene="sul2"
                     /locus_tag="IT904_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP3194281.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1241..2101)
                     /locus_tag="IT904_004616"
     CDS             complement(1241..2101)
                     /locus_tag="IT904_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAP3194282.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(2284..>2742)
                     /locus_tag="IT904_004617"
     CDS             complement(2284..>2742)
                     /locus_tag="IT904_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3194283.1"
                     /translation="FTDKASGSSTDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMI
                     QLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKL
                     KGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          681 a          742 c          679 g          640 t
ORIGIN      
        1 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
       61 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
      121 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacca
      181 aaaaaaatat tagttcgatt caatggaggt tccttcagtt ttctgatgaa gcgcgaatat
      241 agagaaatat cccgaatgtg cagttaacga attcttgcgg tttctttcag cgccgccaat
      301 accgccagcc cgtcgcgcaa ggggcgcggc tcgtgtgtgc ggatgaagtc agctccacct
      361 gcggcggcgg caagctctgc agcgagtgtc gcggccccga catcccccgg accacggcct
      421 gtgagcgcgc gcagaaagga tttgcgcgaa acagacagaa gcaccggcaa atcgaagcgc
      481 agccgcaatt catcgaaccg cgccagcacc gagagcgagg tttcgggagc agcccccaga
      541 aaaaacccca tgccgggatc aaggacaagg cggttgcgtt tgataccggc acccgtcagc
      601 gccgcgatgc gcgcgtcaaa gaacgccgca atgtgatcca tgatgtcgcc agcgggtgcc
      661 tcgcgccgat ctgcctgccc gtcttgcacc gaatgcataa cgacgagttt ggcagatgat
      721 ttcgccaatt gcggatagaa cgcagcgtct ggaaaaccgc gaatatcatt gagataggcc
      781 acaccacgcg acaaggcata ggcttgcgtc gcgggttgat aactgtcgag cgagacggga
      841 atgccatctg ccttgagcgc gtccagcacc ggcgcgatac gcgcgatttc tgtgtcggac
      901 gaaacaggcg cggcgtcggg attgctggat gccggaccga ggtcgatcac atctgccccc
      961 tcggccatca gcttacgcgc ctgcgcaatg gctgcgtctg gcgccagata ccggcctcca
     1021 tcggagaaac tgtccgaggt tatgttgacg atgccgaaaa tgatgagcga tttattcatg
     1081 ggggcttcta taaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1681 cgatcgttgt cagaagtaag ttggcagcag tgttatcact catggttatg gcagcactgc
     1741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1861 gggataatac cgcaccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acgaaaatgt tgaatactca
     2101 tactcttcct ttttcaatat tattgaagca tttaccaggg ttattgtctc atgagcggat
     2161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2221 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     2281 gtatcacgag gccctttcgt cttcaagaat tttataaacc gtggagcggg caatactgag
     2341 ctgatgagca atttccgttg caccagtgcc cttctgatga agcgtcagca cgacgttcct
     2401 gtccacggta cgcctgcggc caaatttgat tcctttcagc tttgcttcct gtcggccctc
     2461 attcgtgcgt tctaggatcc tccggcgttc agcctgtgcc acagccgaca ggatggtgac
     2521 caccatttgc cccatatcac cgtcggtact gatcccgtca tcaatgaacc ggactgccac
     2581 gccctgagcg tcaaattcct ttatcagttg gatcatatcg gcagtgtcgc ggccaagacg
     2641 gtcgagcttc ttaaccagaa tgacatcacc ttcctccacc ttcatcctca gcaaatccag
     2701 cccttcccgg tctgttgaac tgccggatgc cttatcggta aa
//
LOCUS       DACAPB010000310         2742 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.316, whole genome shotgun sequence.
ACCESSION   DACAPB010000310 DACAPB010000000
VERSION     DACAPB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.316"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            128..415
                     /locus_tag="IT904_004618"
     CDS             128..415
                     /locus_tag="IT904_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194284.1"
                     /translation="MRFRVVCLLLTTSGEVVSFLFRITKKEIYMTKEEFVSYIFDKTV
                     EMYAATYGSCNPLNKPEGKDDFDKIYRFLEDRYIKRLEDAGIKSPVKSPLS"
     gene            complement(384..749)
                     /locus_tag="IT904_004619"
     CDS             complement(384..749)
                     /locus_tag="IT904_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194285.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPGVLAGEICTEISSELLPVIREAICRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
     gene            complement(761..886)
                     /locus_tag="IT904_004620"
     CDS             complement(761..886)
                     /locus_tag="IT904_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAP3194286.1"
                     /translation="MPPVCGVFPNQDGTFTAMTYTKSKTFKTESGARCWLARNTD"
     gene            874..978
                     /locus_tag="IT904_004621"
                     /pseudo
     CDS             874..978
                     /locus_tag="IT904_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            962..1483
                     /locus_tag="IT904_004622"
     CDS             962..1483
                     /locus_tag="IT904_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194287.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            1464..2429
                     /locus_tag="IT904_004623"
     CDS             1464..2429
                     /locus_tag="IT904_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194288.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GNELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            2470..>2742
                     /locus_tag="IT904_004624"
     CDS             2470..>2742
                     /locus_tag="IT904_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP3194289.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGYVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVV"
BASE COUNT          768 a          591 c          718 g          665 t
ORIGIN      
        1 cggtgtcctc aacggggaag gaataacccc gccatactta ccgccgcgcc atttcgcggg
       61 ttgccacaac cggaagcgca cggtcgaatt aaatttaacg acaccgtaca gtgagacgaa
      121 cttcgccgtg cgctttcgtg ttgtgtgcct gcttttaacc acgtcaggcg aggtggtatc
      181 cttcttattc cgaataacca agaaggaaat ctatatgact aaagaagaat ttgtctctta
      241 tatttttgat aaaacggttg aaatgtatgc cgctacttac gggtcctgta atcctctgaa
      301 taaaccagag gggaaagatg atttcgacaa aatttaccgc ttcttggagg accgctatat
      361 caaaaggtta gaggacgcag ggatcaaatc cccagtgaag tcaccattgt cctgagaact
      421 tgcaggacgt catgatcgta acttccatcc aaaccgcgac ggcaaattgc ttctcgtatt
      481 accggaagca gttcgctgga aatctcggtg catatttcac ctgctaatac gcctggctca
      541 agtgagaata ttggtgaact tacggtcttg gtctcgacag tttcagagtc agtgccaaca
      601 ttataaagct caacgaaagc ggtcttgatt ttccgggcca gatcttttgc tggctcgctt
      661 gcaatatctt tcccgatttc tcgcagcaca gaatgcaatg tatgagctgc tgttttctgt
      721 acatcagacg gtaaatcttt aaattccatc gtcagcctca tcagtcagtg tttctggcta
      781 accagcaacg cgcgccagat tcggttttaa acgttttgct tttggtatat gtcatcgcgg
      841 tgaacgtacc gtcctggttg gggaacacgc cacatacagg aggcatctgg cggcgagctt
      901 ctgtatgatc ccaaggttta tgacgaatat cgtaagacga agcgggcggg gcggttgaac
      961 aatgaaaatc actcctgaac aggctcgtga ggctctggat gcctggatat gtcgaccagg
     1021 aatgacacag gagcaggcga cgatattaat cactgaagca ttctgggctt tgaaagagcg
     1081 cccgaacatc gatgttcagc gtgtcacaga tgaaggtggc gcggttgatc agcgagcgct
     1141 tggcgttaat cgagtgaaga tattcgaacg ctggaaggct atcgacacta gggataagcg
     1201 tgaaaagttc acggcgctag tgcctgcaat tatggaggct atccggatta atgatttcag
     1261 gttgtatcgt gaaattagtg acggaaaaag catcacgtac atgatcgccg ggttaaacaa
     1321 agaatatggc gatgtggtgg agtccggact gctttttgct gatcctgccg tagtggatcg
     1381 tgaaactgac gaacttatag aaaaagcaat tgctttcaaa cttgcgtatc gacagcaata
     1441 ccaacaaaaa gctggatgga attatgagcc ttctttttgc tgaacgccca ctggttataa
     1501 acacacagct tgcgatgaag attggcttaa atgaagccat tgtgttgcag cagttgcatt
     1561 actggttgag agataccaat tccggcatgg aatgtgatgg tgttcgctgg atttacaaca
     1621 caacggaaca atggctggaa cagttcccat tctggtcaga gtcaacgtta aagcgcgcgt
     1681 ttgcaagtct gaaaacgctg gggcttttgc gttgtgaaaa gctcaataaa tcaaagcgcg
     1741 atatgaccaa tttctacacg atcaactatg ggaacgagct tttagatggt ggcaaattga
     1801 acgaatccat cggttcaaaa tgcgccgctc catcaggtca aaatgacacg atggaagagg
     1861 tcaaaatgaa acgctccatt ggttcaaaac gacccaatgt catcgggtca aaatggcctg
     1921 atgatcttac agagaataca acagagatta ctacagagaa taaaaacact tttcgtccgg
     1981 aagcttcgca accggacccg cagaccgctg aacaggattt tttaatccgg aaccccgacg
     2041 cggttgtgtt tagtgcgaaa aaacgccagt ggggtagcag ggaggatttg gcgtgtgcgc
     2101 agtggatttg ggggcggatc gtgaaccttt acgaacaggc tgccagcgac gatggagaga
     2161 tcatgcgacc aaaagagcct aactggacag cctgggccaa tgacgtgcgc acaatgcgga
     2221 tgctggatgg cagaagccac agacaaattt gcgaaatgtt tggtcgggta cagcgagatc
     2281 cattctgggt aaaaaacatc atgagcccgt caaaactccg cgaaaaatgg gacgaactgg
     2341 tcatccgctt gggacgttca cctgtacagc gttgtgtgaa tcatatttct gaaccggata
     2401 cagaaattcc gcctggtttc aggggataag tgttgatttc aggtcatgag gtaattttaa
     2461 ggaggacttg tggcaaaagt ttttacacag gaagagcggg aaaaaattaa agagcaggtg
     2521 gtcgaacttg tgcgccagag cggtcgcgag acattacgac agttggaagc taaaacaggt
     2581 gcgacaagat atctgatgag cattcttgcc agagagctgg ttgccagtgg ttatgtatac
     2641 aactccggct acgggttatt cccgtctgaa caggcacgaa aggactggca aaatgcccgc
     2701 aaaaaactct ctagggcgaa ggtgaagaaa cccgctgtgg tt
//
LOCUS       DACAPB010000311         2710 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.317, whole genome shotgun sequence.
ACCESSION   DACAPB010000311 DACAPB010000000
VERSION     DACAPB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.317"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..637
                     /gene="allD"
                     /locus_tag="IT904_004625"
     CDS             <1..637
                     /gene="allD"
                     /locus_tag="IT904_004625"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAP3194290.1"
                     /translation="QSDPMVVPFGGAEIYYGTNPLAFAAPGEGDEILTFDMATTVQAW
                     GKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALLPAAGPKGYGLMMMIDVLSGVLL
                     GLPFGRQVSSMYDDLHAGRNLGQLHLVINPNFFSSSELFRQHLSQTMRELNAITPAPG
                     FNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISDALYNTSYETKNPFAQ"
     gene            659..1802
                     /gene="allC"
                     /locus_tag="IT904_004626"
                     /pseudo
     CDS             659..1802
                     /gene="allC"
                     /locus_tag="IT904_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
     gene            1813..2598
                     /gene="allE"
                     /locus_tag="IT904_004627"
     CDS             1813..2598
                     /gene="allE"
                     /locus_tag="IT904_004627"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAP3194291.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVITGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
BASE COUNT          680 a          641 c          702 g          687 t
ORIGIN      
        1 ccagtccgat ccaatggtgg tgccgtttgg cggcgcggaa atttactacg gtactaaccc
       61 actggccttt gccgcgccgg gagaaggcga cgagatcctt acctttgata tggcgactac
      121 cgtacaggca tggggaaaag tgctcgacgc ccgctcgcgt aatatgtcta tcccggatac
      181 ctgggcggtc gataaaaacg gtgcaccaac aaccgatccg ttcgcggtac atgctctgct
      241 ccccgccgcc gggccgaaag ggtatggcct gatgatgatg attgacgtcc tctcaggcgt
      301 cttactcggc ttaccgttcg ggcgacaggt tagttcgatg tatgacgatt tacacgccgg
      361 gcgtaatttg gggcaattac atttagttat taacccgaac tttttctcct ccagcgaatt
      421 attccgtcaa catcttagcc agaccatgcg cgaattaaat gccattaccc ccgcgcccgg
      481 ttttaaccaa gtttattatc ccggacagga tcaggatatt aaacaacgca aagccgccgt
      541 cgaaggcatc gaaattgttg atgatattta ccagtattta atttccgacg cgctttataa
      601 cacgtcatac gaaacgaaaa atccctttgc gcaataatta ttgagacagg actttcttat
      661 gattacacat tttcgtcaag ccatagaaga agcgctgccc tggctttcct cttttggcgc
      721 tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg aaacccagca
      781 gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg aagtagggaa
      841 tttatacggt cgcctgaatg gtaccgaata tccacaggaa gtgattctga gcggttcgca
      901 tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc tggcggcgtg
      961 gctggcaatt gactggctga aaacgcaata cggcgcaccg ttacgtacgg tcgaagtggt
     1021 ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca gtaaaaatat
     1081 ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag gaaatagttt
     1141 tgtcgatgcg atgaaggctt gcggatttac tctgccggac gccccgctaa ctccgcgtca
     1201 ggatattaaa gcctttgtcg aactgcatat cgaacagggc tgtgtgctgg aaagtaatgg
     1261 gcaatcaatt ggcgtggtga atgcaattgt cgggcaacgt cgctatacgg tgacgctgaa
     1321 cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata cggtttacgc
     1381 tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg atccgctggt
     1441 tctgaccttt gggaaagtag agccgcgccc gaatacggtg aatgtggtgc cggtgcgatg
     1501 aaatggacat tggtattgat atcgatttat ggatggacga agaacccgtg ccgatgaata
     1561 aggacctggt cgccaccctg acagaattgt gtgaaagtga aaaactgaat taccgggtga
     1621 tgcacagtgg tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtgccg acctgcatga
     1681 ttttcattcc cagcatcaac gggatcagcc ataacccggc ggaacgcacc aatattaccg
     1741 accttgccga aggggtcaaa acgttggcac tcatgcttta tcaacttgcc tggcagaaat
     1801 aaggagtcat aaatgggata tttaaataac gtcaccggtt accgcgatga tttactggct
     1861 aaccgtgcga ttgtgaaaca cggtaatttc gcactgttaa ccccggacgg tctggtaaaa
     1921 aatattattc cgggctttga aaattgtgac gcgacaatcc tctccacgcc aaagctgggt
     1981 gcctcttttg ttgattatct ggtcacactg catcaaaacg gcggcaacca acagggcttc
     2041 ggtggcgaag gcattgaaac gttcctgtat gtgatcactg gaaatatcac tgccaaagcc
     2101 gaaggcaaaa catttgcctt aagcgaaggt ggctatcttt attgcccgcc aggctcctta
     2161 atgacgtttg ttaacgccca ggccgaagac agccaaatct ttttatataa gcgccgctat
     2221 gttccggtag aaggccatgc accgtggctg gtttctggca atgccagcga actggaacgc
     2281 attcactatg aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa agagttaggt
     2341 tttgatatga acatgcatat cctctctttt gcaccaggtg ccagccacgg ttatatcgaa
     2401 acacatgttc aggaacacgg tgcctatatt ctttccggtc agggggttta taacctcgac
     2461 aataactgga tcccggtgaa aaaaggcgat tacatcttta tgggcgctta ttctttacag
     2521 gctggttatg gtgtagggcg tggtgaagcg ttcagctata tttactcgaa agattgcaac
     2581 cgcgacgtag agatttaagt cataccactc gcctgattat ttttagctat gttggatttt
     2641 gccacggttt ataccgtggc tttttttgta acattgcctg atgcgcttcg cttatcaggc
     2701 ctacatgatc
//
LOCUS       DACAPB010000312         2685 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.318, whole genome shotgun sequence.
ACCESSION   DACAPB010000312 DACAPB010000000
VERSION     DACAPB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.318"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            105..548
                     /gene="holC"
                     /locus_tag="IT904_004628"
     CDS             105..548
                     /gene="holC"
                     /locus_tag="IT904_004628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAP3194292.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            548..>2685
                     /gene="valS"
                     /locus_tag="IT904_004629"
     CDS             548..>2685
                     /gene="valS"
                     /locus_tag="IT904_004629"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP3194293.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEDQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQF"
BASE COUNT          675 a          704 c          734 g          572 t
ORIGIN      
        1 tccctaaggg agagggaacc gatcgagcgc aatagaaaat cattgctcat cttttagggt
       61 gaggaatgca gcaatattta aatccaccac aagaagcccc atttatgaaa aacgcgacgt
      121 tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag caactggtgt
      181 gtgaaattgc cgcagaacgt tggcgcagtg gtaagcgcgt actcatcgcc tgtgaagatg
      241 aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa agctttgttc
      301 cgcataattt agcgggagaa ggaccgcgcg gcggtgcgcc ggtggagatc gcctggccgc
      361 aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc tttgcagatt
      421 ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct ctgaaacaac
      481 tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat acggcaacct
      541 ggaaataatg gaaaagacat ataacccaca agatatcgaa cagccgcttt acgagcactg
      601 ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt tctgcatcat
      661 gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc agcaaaccat
      721 catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg
      781 tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg ccgcagaaga
      841 aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct gggaatggaa
      901 agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact ccgtagactg
      961 ggagcgtgaa cgcttcacta tggacgaagg cctgtccaat gcggtgaaag aagttttcgt
     1021 tcgtctgtat aaagaagacc tgatttaccg tggcaaacgc ctggtaaact gggatccgaa
     1081 actgcgcacc gctatctctg acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg
     1141 gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gatggtaaag attatctggt
     1201 ggttgcgact actcgtccgg aaaccctgct gggcgatacc ggcgtggccg ttaacccgga
     1261 agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg ttaaccgtcg
     1321 tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaagat
     1381 caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc tgccgatgat
     1441 caacatcctg acctttgacg gcgatatccg tgaaagtgcc caggtgttcg ataccaaagg
     1501 taacgaatct gacgtttatt ccagcgagat ccccgcagaa ttccagaaac tggagcgttt
     1561 tgctgcacgt aaagcggtcg ttgctgcggt tgacgcactc ggcctgctgg aagaaattaa
     1621 accacatgac ctgaccgttc cttatggcga ccgtggtggc gtggtcatcg aaccgatgct
     1681 gaccgaccag tggtacgtgc gtgccgatgt cctggcgaaa ccggcagttg aagcggttga
     1741 gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct cctggatgcg
     1801 cgatattcag gactggtgta tctctcgtca gttgtggtgg ggtcaccgta tcccggcatg
     1861 gtatgacgaa gcgggtaacg tttatgttgg ccgcaacgaa gacgaagtgc gtaaagaaaa
     1921 taacctcggt gctgatgttg tcctgcgtca ggacgaagac gttctcgaca cctggttctc
     1981 ttctgcgctg tggaccttct ctacccttgg ctggccggaa aacaccgacg ccctgcgtca
     2041 gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct ggattgcccg
     2101 catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt
     2161 ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggccagaaga tgtccaaatc
     2221 caagggtaac gttatcgacc cactggatat ggttgacggt atttcgctgc cagaactgct
     2281 ggaaaaacgc accggcaata tgatgcagcc acagctggcg gacaaaatcc gtaagcgcac
     2341 cgagaagcag ttcccgaacg gtattgaacc gcacggtact gacgcgctgc gcttcaccct
     2401 ggcggcgctg gcttctaccg gtcgcgatat caactgggat atgaaacgcc tggaaggtta
     2461 ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga acaccgaaga
     2521 tcaggattgc ggcttcaacg gcggcgagat gacgctgtcg ctggcggatc gctggattct
     2581 ggcggagttc aaccagacca tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga
     2641 tatcgccgca ggcattctgt atgagttcac ctggaaccag ttctg
//
LOCUS       DACAPB010000313         2584 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.320, whole genome shotgun sequence.
ACCESSION   DACAPB010000313 DACAPB010000000
VERSION     DACAPB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.320"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            279..1943
                     /gene="asnB"
                     /locus_tag="IT904_004630"
     CDS             279..1943
                     /gene="asnB"
                     /locus_tag="IT904_004630"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAP3194294.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2324..2400
                     /locus_tag="IT904_004631"
     tRNA            2324..2400
                     /locus_tag="IT904_004631"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2358..2360,aa:Met,seq:cat)
     gene            2409..2493
                     /locus_tag="IT904_004632"
     tRNA            2409..2493
                     /locus_tag="IT904_004632"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2443..2445,aa:Leu,seq:tag)
BASE COUNT          675 a          639 c          645 g          625 t
ORIGIN      
        1 ggccggataa ggcgttcacg ccgcatccgg catgaacaaa ttgcactttg tcagcaattt
       61 ggcccccgta aaatataacc attcaataaa acccgctaaa aattatcgat tcatcaataa
      121 atacatcctt acaccagacc ttttttcacc aatcgacaat caccattacg ttttttattt
      181 tttcaccgcc aaatcgcttg cgcacagtgc tgctttgcgg cattttttta aacaagcaaa
      241 cacaacaagc aacaaatacc aggttaacgg agaaggttat gtgttcaatt tttggcgtat
      301 tcgatatcaa aacagacgca gttgagctgc gtaagaaagc actcgagctg tcacgcctga
      361 tgcgtcatcg tggcccggac tggtccggta tttatgccag cgataacgcc attctcgccc
      421 acgaacgtct gtcaattgtt gacgttaacg ctggggcgca acctctctac aaccaacaaa
      481 aaactcatgt gctggcggta aacggtgaaa tctacaacca ccaggcactg cgcgccgaat
      541 atggcgatcg ttatcagttc cagaccggat ctgactgtga agtgatcctc gcgctgtatc
      601 aggaaaaagg gccggaattt ctcgacgact tgcagggcat gtttgccttt gccttgtacg
      661 acagcgaaaa agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata
      721 tgggctatga cgaacacggt cagctgtatg tggcctcaga aatgaaagcc ctggtgccag
      781 tttgccgcac gattaaagag ttcccggcgg ggagctattt gtggagccag gacggcgaaa
      841 tccgttctta ttatcatcgc gactggttcg actacgatgc ggtgaaagat aacgtaaccg
      901 acaaaaacga gctgcgtcag gcacttgaag attccgttaa aagccatctg atgtctgatg
      961 tgccttacgg tgtgctgctt tctggtggtc tggattcctc aattatttcc gctatcacca
     1021 agaaatacgc agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccgcagt
     1081 tacactcctt tgctgtaggt ctgccgggtt caccggatct taaagcagcc caggaagtgg
     1141 caaaccatct gggcacggtg catcacgaaa ttcacttcac tgtacaggaa ggtctggatg
     1201 ccatccgcga cgtgatttac cacatcgaaa cttatgatgt gaccacaatt cgcgcttcaa
     1261 caccgatgta tttaatgtcg cgtaagatca aggcgatggg cattaaaatg gtgctgtccg
     1321 gtgaaggttc tgatgaagtg tttggcggtt atctttactt ccataaagcg cccaacgcta
     1381 aagaactgca tgaagagacg gtgcgtaaac tgctggccct gcatatgtat gactgcgcgc
     1441 gcgccaacaa agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaaa
     1501 aattccttga tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaaa
     1561 tggaaaaaca catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc
     1621 ggcagaaaga gcagttctcc gatggcgtcg gttacagttg gatcgacacc ctgaaagaag
     1681 tggcggcgca gcaggtttct gatcagcaac tggaaactgc ccgcttccgc ttcccgtaca
     1741 acacgccaac ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa ctgttcccgc
     1801 ttccgagcgc cgctgagtgc gtgcctggcg gtccttccgt cgcgtgttct tccgctaaag
     1861 cgatcgaatg ggatgaagcg ttcaagaaaa tggacgatcc atctggtcgt gcggttggtg
     1921 ttcaccagtc ggcatataag taagaaatgc gaaacagccc cggtgaaatc cggggctgtt
     1981 tcagttattg aggtgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca
     2041 catcactgtt tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac
     2101 tattgttgaa ttccgaattt catcctgtac gacaaaagaa tggctgtagg gtggggaaaa
     2161 aacacaaagt aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaac
     2221 agtcactttc gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat
     2281 aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg tagctcagtt
     2341 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
     2401 tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa
     2461 ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg tacggatggg
     2521 gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg
     2581 tacc
//
LOCUS       DACAPB010000314         2552 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.321, whole genome shotgun sequence.
ACCESSION   DACAPB010000314 DACAPB010000000
VERSION     DACAPB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.321"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(491..904)
                     /locus_tag="IT904_004633"
                     /pseudo
     CDS             complement(491..904)
                     /locus_tag="IT904_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            902..1159
                     /locus_tag="IT904_004634"
                     /pseudo
     CDS             902..1159
                     /locus_tag="IT904_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1393..2250)
                     /gene="sitD"
                     /locus_tag="IT904_004635"
     CDS             complement(1393..2250)
                     /gene="sitD"
                     /locus_tag="IT904_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAP3194295.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(2247..>2552)
                     /gene="sitC"
                     /locus_tag="IT904_004636"
     CDS             complement(2247..>2552)
                     /gene="sitC"
                     /locus_tag="IT904_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAP3194296.1"
                     /translation="VSTVAALQTVGAFLVICLVVTPGATAWLLTDRFPRLLMIAVTIG
                     SVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRRAHKALEDRS"
BASE COUNT          750 a          639 c          568 g          595 t
ORIGIN      
        1 agtaccaata attaacccgg caaggaggtt caccaccagt gtacctggag gtagatttgg
       61 aaaaagactg ttaaagcgtg tggacagtaa ccagcgcaga gtacatccaa cagaaccgcc
      121 gataatgacg gcaaaaagtg atttaatcat gtacccctca taaaataatt ttttatcgta
      181 acaatatcag aatatgttct catttgactg atattgttaa tttatatcct ttgactacat
      241 gcaagataag ggaaggttct atttattgac attaataccc tcgacaattg ctgagcagaa
      301 gcccccacga caagtgacag aatgacaaca tctacagtgg ggagcgtttt atcgatgaac
      361 aagcaaccaa gaagtaacac ggcataggtg acgacaatgc cagtaagcat agaaataaca
      421 aaaacgtgag ctttcatcat tcctccagaa taaccccgac gtgtaagcct gggggattgg
      481 cacaataatg tcatttaaag gcagactttc cagcaaattg cgccgatgaa cccagttcat
      541 cctcaattct cataagctgg ttatattttt caatacgctc accccggcat ggagctcccg
      601 ttttgagatg acctgcgcgc attgcaactg tcatatcggc gataaagctg tccactgtct
      661 caccactgcg gtgtgagata aaggtgcccc agttgttgtc atggcaaagt tgtaccgcat
      721 caaaggtttc actcagggaa ccaatctggt tgagtttaat caatgctgaa ttggcaaggt
      781 tttcgtcaat gccgcgctga atatacttta cgttggtcac aaacagatcg tcgccaacca
      841 attctatctg ctctcccaat gcaccatgca ggattttcca tccagcccag tcatcttcag
      901 ctaccgttaa gggcaggaag atcaactagg tgctctgggg ctggtgacca atgcggttgt
      961 gctgtgaaac acactttaca tgcaggaggc gttgtcacat cttcgcaaat cgggagaaac
     1021 tcctgaggag tagcatccgg caagattatc gctgctgata catagccata taaatatgct
     1081 ggggcattat acgtcttcgt taccggaaga tattcttaag ggtgagttaa gatcattaaa
     1141 cttcaataca aacaattaat taaactctta gcgtggtttt ttacatcact ggccctcgcc
     1201 ccccaatttt ctctatgttt tgccactact tccctcattt tactgcgctg tttgctaccc
     1261 ctgagaatcg acattacttg ctatccagtc cgcagcaact ctctggcaac aaaacttatc
     1321 agaatcataa gtctgtgcat tattcgttca agcgcatcgc caataagcgg atgttgctgg
     1381 ccgagtgttc tgctaatcgg atgataacaa atcccgtggt tcggcatttt cctttgtgcg
     1441 tgcgttgatg ctggtaacgg taaacgtcat gatgaacatg accgcgaata agacaacaat
     1501 tgtcggagct ggcgcgctgt cgataaaaaa ggacagccag acaccagtca ttgagaccaa
     1561 tatcgatatg ccagtcgcca gcaggagcgc aatatgaaag cgttgtgtca ggagtaccgc
     1621 aatggcacca ggcgcaatga gaagagaaat agagagaatg atacctaccg ctttcagcgt
     1681 cgccacaatg gtcagggaga ccatgcacag cagcccataa tgcagccatc tcgtatgcaa
     1741 accgcttgcc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc gccattttac
     1801 gctaataaca agtgtaacca gtccggcaat aatcaccgtc tggattatat cgccgatggt
     1861 tatcccgagc atgtcgccga aaagaatatg atcaagatgt acgtctggtt tgactgcaat
     1921 atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccatca ctgtatcttg
     1981 cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcacagaata ctccggcgac
     2041 gaatgcccct gtagccagag gtaaccccag aatccaggca agcacgatgc caggaaaaac
     2101 cgcatgactc atcgcgtcac ccatcagtgc ccaacttttc aggaccagaa agaccgacag
     2161 cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca taaaggtgaa
     2221 ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt atgcgcacgc
     2281 cgacgatttg ccaaaagccc atgggtgggc gcaaagacaa atgctaaaag aaacagcagt
     2341 gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact gacccacgcg
     2401 ccgaggaaac tggtcacact gccaatagtt acagcaatca tgagcaggcg gggaaaacga
     2461 tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac cagaaaggca
     2521 ccaaccgttt gcagcgccgc aacggtcgaa ac
//
LOCUS       DACAPB010000315         2517 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.322, whole genome shotgun sequence.
ACCESSION   DACAPB010000315 DACAPB010000000
VERSION     DACAPB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.322"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            162..1145
                     /gene="pheS"
                     /locus_tag="IT904_004637"
     CDS             162..1145
                     /gene="pheS"
                     /locus_tag="IT904_004637"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP3194297.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            1160..>2517
                     /gene="pheT"
                     /locus_tag="IT904_004638"
     CDS             1160..>2517
                     /gene="pheT"
                     /locus_tag="IT904_004638"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP3194298.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WR"
BASE COUNT          580 a          656 c          730 g          551 t
ORIGIN      
        1 gcagccagac tggacacggc cagcgcggag aaaccggagc gtatttaagt acgtgagaat
       61 ttcgagcaca gcccgggacc aaaatggcaa gtaaaatagc ctgatgggat aggctctaag
      121 tccaacgaac cagtgtcacc actgacacaa tgaggaaaac catgtcacat ctcgcagaac
      181 tggttgccag tgcgaaggcg gccattagcc aggcgtcaga tgttgccgcg ttagataatg
      241 tgcgcgtcga atatttgggt aaaaaagggc acttaaccct tcagatgacg accctgcgtg
      301 agctgccgcc agaagagcgt ccggcagctg gtgcggttat caacgaagcg aaagagcagg
      361 ttcagcaggc gctgaatgcg cgtaaagcgg aactggaaag cgctgcactg aatgcgcgtc
      421 tggcggcgga aacgattgat gtctctctgc caggtcgtcg cattgaaaac ggcggtctgc
      481 atccggttac ccgtaccatc gaccgtatcg aaagtttctt cggtgagctt ggctttaccg
      541 tggcaaccgg gccggaaatc gaagacgatt atcataactt cgatgctctg aacattcctg
      601 gtcaccaccc ggcgcgcgct gaccacgaca ctttctggtt tgacactacc cgcctgctgc
      661 gtacccagac ctctggcgta cagatccgca ccatgaaagc ccagcagcca ccgattcgta
      721 tcatcgcgcc tggccgtgtt tatcgtaacg actacgacca gactcacacg ccgatgttcc
      781 atcagatgga aggtctgatt gttgatacca acatcagctt taccaacctg aaaggcacgc
      841 tgcacgactt cctgcgtaac ttctttgagg aagatttgca gattcgcttc cgtccttcct
      901 acttcccgtt taccgaacct tctgcagaag tggacgtcat gggtaaaaac ggtaaatggc
      961 tggaagtgct gggctgcggg atggtgcatc cgaacgtgtt gcgtaacgtt ggcatcgacc
     1021 cggaagttta ctctggtttc gccttcggga tggggatgga gcgtctgact atgttgcgtt
     1081 acggcgtcac cgacctgcgt tcattcttcg aaaacgatct gcgtttcctc aaacagttta
     1141 aataaggcag gaatagatta tgaaattcag tgaactgtgg ttacgcgaat gggtgaaccc
     1201 ggcgattgat agcgatgcgc tggcaaatca aatcactatg gcgggcctgg aagttgacgg
     1261 tgtagaaccg gttgccggca gcttccacgg cgtggtcgtt ggtgaagtgg ttgagtgtgc
     1321 gcagcatccg aacgctgaca aactgcgtgt gacaaaagtg aatgtcggcg gcgatcgcct
     1381 gctggacatc gtctgcggtg cgccaaactg ccgtcagggc ctgcgtgtgg cggtagcgac
     1441 cattggtgct gttctgccgg gtgatttcaa aattaaagcg gcgaaactgc gtggcgaacc
     1501 gtctgaaggg atgctgtgct ccttctctga actgggcatt tctgacgatc acagcggcat
     1561 tatcgaactg cctgcggatg cgccgattgg caccgatatc cgtgaatacc tgaaacttga
     1621 tgacaacacc atcgaaatca gcgtgacgcc aaaccgtgcc gactgcttag gcatcattgg
     1681 tgttgcgcgt gacgttgccg tgctgaacca gctgccgctg gttcaaccgg aaatcgttcc
     1741 ggttggtgcg accatcgacg acacgctgcc gattacagtc gaagcgccgg aagcctgccc
     1801 gcgttatctt ggccgtgtgg taaaaggcat taacgttaaa gcgccaactc cgctgtggat
     1861 gaaagaaaaa ctgcgtcgtt gcgggatccg ttctatcgat gcagttgttg acgtcaccaa
     1921 ctatgtgctg ctcgaactgg gccagccgat gcacgctttc gataaagatc gcattgaagg
     1981 cggcattgtg gtgcggatgg cgaaagaggg cgaaacgctg gtgctgctcg acggtactga
     2041 agcgaagctg aatgctgaca ctctggtcat cgccgaccac aacaaggcgc tggcgatggg
     2101 cggcatcttc ggtggcgaac actctggcgt gaatgacgaa acacaaaacg tgctgctgga
     2161 atgcgcgttc tttagcccgc tgtctatcac cggtcgtgct cgtcgtcatg gcctgcatac
     2221 cgatgcgtct caccgttatg agcgtggcgt tgatccggca ctgcagcaca aagcgatgga
     2281 acgtgcgacc cgtctgctga tcgacatctg cggtggtgag gctggcccgg taattgatat
     2341 caccaacgaa gcaacgctgc cgaagcgtgc aaccatcact ctacgtcgta gcaaactgga
     2401 tcgcctgatc ggccatcata ttgcggatga gcaggtaact gacattctgc gtcgtctcgg
     2461 ctgcgaagtg accgaaggca aagacgaatg gcaggcagtt gcgccgagct ggcgttt
//
LOCUS       DACAPB010000316         2442 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.324, whole genome shotgun sequence.
ACCESSION   DACAPB010000316 DACAPB010000000
VERSION     DACAPB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.324"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(101..1069)
                     /locus_tag="IT904_004639"
     CDS             complement(101..1069)
                     /locus_tag="IT904_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination enzyme"
                     /protein_id="HAP3194299.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYC
                     SVSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQW
                     LEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAE
                     SVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVE
                     DQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGL
                     SREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
     gene            1753..2256
                     /locus_tag="IT904_004640"
     CDS             1753..2256
                     /locus_tag="IT904_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194300.1"
                     /translation="MAKRGAGVNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPP
                     YVKLYIADIAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTF
                     KNIIAALVDKQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQT
                     KVMDDEC"
BASE COUNT          611 a          629 c          565 g          637 t
ORIGIN      
        1 aaaaaaacat aattaccatc tgaatgatta attttttcgt ggtgaatgcc gcaaatgacg
       61 tcactttgcg gcgttagccc tatcctgcat cgcatcaaaa ctaacgctcc ataccaccca
      121 tgccacgcga tcgcgtctgt cgctgttttt cacgttcaat acgctgctgt ttttcctttt
      181 cctggttttg ctgcttaaac cgctgagcca tcatcaggat ttcggtcatc tgctctctgg
      241 aaagcccctt aaaaggctct gtgacgctct gtaggcgttt ctgctgcgca tccatagtct
      301 tctgtagctc acgcgctctt tttgcgttct gggcgctctg agaggccata gcgatggttc
      361 ctgcaaagcc atcgttcact gcgtggctta gacgtttggc tatggctgcc tgatcttcca
      421 cgtctttggt gaaaatgccc ttccgcaata cacgcggctc cagcgcctcc ggcgagatcg
      481 atacctggct acgggtgcca cggtttatcg attcgtagta gtgctgaact gtctgatgcg
      541 tggcgcggct tccctcgatg ccgcgctcaa gacccagttt ttttactgat tcagcgtagg
      601 tgctctgatc ttcacgcatc tttgaccgcc cgccaataaa ctctttcgcg gacagccgtc
      661 catcctgcgt caatggcacc acgaacgcag acaggtgcgg ggtggcctcg tcccggtgaa
      721 ccaccgccgc cacaacacga tccttcccgt atttcttctc cagccattgc tcagagcggg
      781 cgaaaaactc cgcctgctgc cgtggtgtgg cctctttcca ccactcaggg cttgctgtca
      841 taacgtattc aacggccagt accgcatctt tacggcgttt ttccggcagc agctcccgca
      901 cacgccccat tgcctgatcc gttgatttgg acacagagca gtagttttct ggtgtccgtt
      961 cagcgttagc gttcggcgtc tcacgctctc ggtagcagtg ctgcaacgcg ctggccacgc
     1021 ttcccattcc cgtcagcttt ttgcagcgca ttatcgcgta gttcgccatg ttcccccctg
     1081 attccggcat tcattcagcc aggcggggga gcgcattgcg atacctccgg cgggccacta
     1141 tcaagatgat agtgtactca ctagactttg ctacgcaaaa tcgttcgggc agcaagctgc
     1201 cagaaggtca aaatcaacgg cggtctggca ggtggaaggc gtcgcttcgc gccacccccc
     1261 accactgcca agcctgtttt atccggagtg tcgtcaaggg taaatggcac tcgctacgct
     1321 cgcccttgac gaaatcaggc aatgaaatcg tctttgaaag agcaggaacc gctcgccgca
     1381 gctttggctg aggcgtaccg caggagcaac gcgacgagga acgaacagcc aaagcgagga
     1441 agcggaattt ccactccccg tacatagaaa tgcgtacact ccttgctaaa aaaaggcagt
     1501 ttttcagtgt ctaaaaatgc cgtttttcag cccttatttt cccccttaat atatagatat
     1561 agatatggga acccgcttgt accaagggct agagcgaaga atcgtactca cctcaagtac
     1621 taatcgtact cacctcaagt actaatcgta ctcacctcaa ggactaatcg tactcacctc
     1681 aagtactaat cgtacttgat acaaaggact tgactgtatg tctctttagt acttaaatgc
     1741 gagtactaaa tcatggcgaa acgtggggct ggagtgaata cgaaaaagat ttctaggctt
     1801 gaacggcata catacatgga tgaaaacggt gaggtaaaac aagactctaa gatcgagacc
     1861 ttttccatcc cagccgaacc tccctacgtc aagctataca ttgcagacat tgcgatgatt
     1921 catggtttat caaaatctca aaatgatgtg cttttacaat tggcaatgct gattggatgg
     1981 gatgggtttg tgtctgtatc aaaaactaga tttgaaaaaa ccatacagcc cagggttcat
     2041 attgaatatc agacctttaa aaacataatt gcggctcttg tagataaaca aatattcttc
     2101 cgggcagggc gtggggaact tgaagctaac ccctttcttt ttgcacgggg agactggcca
     2161 tcaatttacg aaagaagaaa gaaaattgag ttaaagatca gttatgacac caatggaaga
     2221 acggtgcaga cgaaggtaat ggatgatgaa tgctgaagat aaacttttta aaaatgtaga
     2281 caggctaatg ctgcatgatt cactaaaaaa gaatgaggtt ctgactgttt gggagcgtac
     2341 ttttttaagc agtgtttaca gcatatatcg tagaactagc ggtaataaat actcaactaa
     2401 gggtttgtca ccaaagcaga aaaacgcagc tttttctatt tt
//
LOCUS       DACAPB010000317         2355 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.325, whole genome shotgun sequence.
ACCESSION   DACAPB010000317 DACAPB010000000
VERSION     DACAPB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.325"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..354)
                     /locus_tag="IT904_004641"
     CDS             complement(<1..354)
                     /locus_tag="IT904_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HAP3194301.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQ"
     gene            299..1084
                     /gene="lafU"
                     /locus_tag="IT904_004642"
     CDS             299..1084
                     /gene="lafU"
                     /locus_tag="IT904_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAP3194302.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLNAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            1155..2210
                     /gene="dinB"
                     /locus_tag="IT904_004643"
     CDS             1155..2210
                     /gene="dinB"
                     /locus_tag="IT904_004643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP3194303.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            2262..>2355
                     /locus_tag="IT904_004644"
     CDS             2262..>2355
                     /locus_tag="IT904_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HAP3194304.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVL"
BASE COUNT          582 a          599 c          651 g          523 t
ORIGIN      
        1 ctgctggaag gcagcatcgg cgctcaggtt gtatttgaag atcccgatac agacgccgcc
       61 gatcaggttg atcgccagaa tcatcatccc ggcgatggcg tccccgcgca caaacttcga
      121 tgccccgtcc atcgcgccgt agaaatcggc ctcgctggca acatctttac gccgggtttg
      181 cgcctgcgcc tggttgatca atccggcgtt aagatcggcg tcaatcgcca tctgtttgcc
      241 gggcatcgcg tctaaggtaa agcgggcaga aacctcggaa atacgctcgg cccctttggt
      301 gacgacaata aagttgattg tcaacagcgt cagtaaatcc gagcgtgaaa gcattatcgc
      361 cgcgctgcac gggcagtcga tttttaacgg tggtgggctg tcgccgttaa ataaaatcag
      421 cccgtctcat ccgccaaaac ctgccaccgt ggcggtgccg gaagagacgg agaaaaaagc
      481 gcgcgacgtc aacgagaaaa cggcgctgct gaagaagaaa tccgccaccg agcttggcga
      541 gctggcaacc agtatcaaca caattgcccg tgatgcgcat atggaagcga atctggagat
      601 ggagattgtt ccccagggat tgcgcgtgct gattaaagat gaccagaatc gcaatatgtt
      661 cgaacgcggc agcgcgcaga ttatgccgtt ctttaaaacg ctgctggtgg agctcgcgcc
      721 agtgttcgac tcgctcgata acaaaattat tattaccggg cataccgatg cgatggccta
      781 caaaaacaat atctacaaca actggaacct ttcgggtgac cgcgcgcttt cggctcgtcg
      841 ggtgctggaa gaggccggaa tgccggaaga taaagtgatg caggtaagcg caatggcgga
      901 ccagatgctg ctgaacgcga agaatccgca aagtgcgggt aatcggcgca tcgagattat
      961 ggtgctgacc aaaagtgcgt ccgatacgct gtatcaatac tttggtcagc atggggataa
     1021 agtggtgcag ccgctggtgc aaaagctgga taagcagcag gtgctttcgc agcgaacgcg
     1081 ctaaatgctg aatctttacg catttctcaa accctgaaat cactgtatac tttaccagtg
     1141 ttgagaggtg agtaatgcgt aaaatcattc atgtggatat ggactgcttt ttcgccgcag
     1201 tggagatgcg cgacaatccc gccctgcgcg atatccctat tgctattggc ggcagccgcg
     1261 aacgtcgggg ggtgatcagc accgccaatt atcccgcgcg taaatttggc gtacgtagcg
     1321 ctatgccgac agggatggcg ctcaaattat gcccgcatct caccttgctt ccggggcgct
     1381 ttgacgccta caaagaagcc tcaaatcata tccgcgaaat cttctcgcgc tacacctcgc
     1441 gtattgaacc gttgtcactg gatgaggctt atctcgacgt caccgatagc gtccattgcc
     1501 acggttctgc gaccctcatc gcccaggaaa tccgccagac gattttcaac gagctgcaac
     1561 tgacggcgtc tgcgggcgtg gcacccgtaa agtttctcgc caaaatcgcc tccgacatga
     1621 ataaacccaa cggccagttt gtgattacgc cggcagaagt tccggcattt ttacaaacct
     1681 taccactggc aaaaatcccc ggcgtcggca aagtctcggc ggcaaaactg gaagcgatgg
     1741 ggctacgaac ctgcggtgat gtacaaaagt gtgatctggt gatgctgctt aaacgctttg
     1801 gcaaatttgg ccgcattttg tgggagcgta gtcaggggat tgacgagcgc gacgttaaca
     1861 gcgaacggtt gcgaaaatcc gtcggcgtgg aacgcacgat ggcggaagat atccaccact
     1921 ggtctgaatg tgaagcgatt atcgagcggc tgtatccgga acttgaacgc cgtctggcaa
     1981 aggtaaaacc tgatttactg attgcccgcc agggggtgaa attaaagttc gacgattttc
     2041 agcaaaccac tcaggagcac gtctggccgc ggctgaataa agctgactta atcgccaccg
     2101 cgcgtaaaac ctgggatgaa cgccgcggcg ggcgcggtgt gcgactggtg gggctgcatg
     2161 tgacgttgct tgatccgcaa atggaaagac aactggtgct gggattatga tgtatactat
     2221 tatgtctatt ctggtgtgca ttattatgag gggatcactg catgcatcga attctcgctg
     2281 aaaaatcggt caatattact gagttacgta aaaacccagc taaatacttt attgatcaac
     2341 cggttgcggt tcttt
//
LOCUS       DACAPB010000318         2263 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.326, whole genome shotgun sequence.
ACCESSION   DACAPB010000318 DACAPB010000000
VERSION     DACAPB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.326"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            326..886
                     /locus_tag="IT904_004645"
     CDS             326..886
                     /locus_tag="IT904_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAP3194305.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLN
                     RQKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPAL
                     TALSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKF
                     LVLPDGVKGENTWTVGDKNAVRLVRE"
     gene            893..1126
                     /gene="mbeD"
                     /locus_tag="IT904_004646"
     CDS             893..1126
                     /gene="mbeD"
                     /locus_tag="IT904_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAP3194306.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNN
                     AILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
BASE COUNT          582 a          542 c          686 g          453 t
ORIGIN      
        1 ccctattacg accgagcaga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac
       61 cattacgggc tgcacgaccc gaacgcaccg gaaaacaggc ggacacttgt caccccgaat
      121 aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt
      181 taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt
      241 tttgaggtgg tgaggacaac caaaagcagc atcagtattg ccgacccgga gggggggcgg
      301 aatctcaggc taaagggggc gctgtatgag caatctttta caaatgggaa cagacttcga
      361 gaagaaactg aaagagcgag ccgcttctac cgagaacatg ctgaacagcg agttcaggaa
      421 gctcgaagag tctgtagaca aggcattgag cttaaacagg cagaaaatca gagacgctat
      481 cagcgaacat acaacatccg tgaaaaagca actggatacg ctgagcacca ccgtgagcat
      541 gcagttcagc accacagagg ccgaactgtc ccggcagcag aaaaaactgc tctggcgggt
      601 gatcaaaggg cggatactgt ttccggcgct gaccgcgctc agcgtcacgg gcgggatttt
      661 cctggggtgc tgggggctga tggagtggca ggaaagcaag attgcgaaaa atatcctgac
      721 tatcagagag caggaaaaca cgctggcgaa actcgaggca aaaacctggg gggtgacgtt
      781 cgtcaatggc gagaacggga aatttctggt gttgccggac ggggtgaaag gggaaaacac
      841 ctggacggtg ggcgacaaga acgccgtgag gctggtcagg gagtaaacga gtatgacgga
      901 actggaaggg catttgctga acgccttaga gcatttgcaa caggactata tgcggcggct
      961 gaacgagtgg gagagcgcct tcgcggaatt gcagaagatg cacgaggtta cgcaacggaa
     1021 caacgcgata ctgaacgagc gcgtcgtgat attgagtcag caggtgcaga gattagccgg
     1081 gcaagtggat cgcttgagtc ggttatttat cgcgaacaac agctaaggga tgaacaggag
     1141 cgtgcccggt acagacatga ggtacaggaa cgcgcggcac gggttcggag tgggccatca
     1201 atggagatgt gaccggagaa gtgacgaaat cttgccattt tgacccgttc gacggctggg
     1261 gcgagcgaaa caaatcgtgt gccgtatttg tgcatagcta tgcaacatcc tgaaaacgca
     1321 tttcacgcgt ttttggggat tcagccaggg gaaaacatgg tttttttact ttttgagcgt
     1381 aaagacgtga aaaatctcgt tattgcactt gcgatctttc gtcggtgggc gtagaacgaa
     1441 aacagctcgc caggaaggcg agctgtttcg ggttcattga ggtctctgac ctcctcaatg
     1501 ttcctgtgga aactgttgag atccacaaca gaaaccagtt cattcagttt gcatgagcct
     1561 tctgtattct tcaagagttt ttcaacagca cagtctccca ggaaccccga aaaaaaactg
     1621 tttttgcaga tacttgcgta tttgcgagaa tcgtttgcgg ttgggggagc tgtgacgacg
     1681 gcgaccgcac gcgtgagagt tccgctaccg gcgcagagag tttcatcgac agcggacacc
     1741 tgatgagttg ccacaaaaac cgtatgtagc gcggctagat tggttgagtt agaccttggc
     1801 ggcagttccc ggtatgtggt ggcgtgacgg gtgagatggt gcggcgaaga cgcatacgtc
     1861 acgcaggcgc gggttcttcc actgacgcgc cccgtgatcg atggccttgg ctccgtccgg
     1921 catatcgaga gcggcaaact acccgtccct tcactgggag gggtgagttg ttgccgttcc
     1981 ctccgacctt ccaccatcca gcatatcgca cgatcccatg tgcaccgcta ctcctgataa
     2041 ctccagtgca aaaaaaaaaa aacggatctg catcacaacg agagtgatcc caccgctcgc
     2101 cgcagcccca gcgaccgaac gtagtgagcg agtgggcgag gaagcggaag agtcacagag
     2161 gcaagcattg tgcacttacg cgcctgtgcc agtgtccgcg tgtgtgttgt ttatcgggcg
     2221 cgctgttttt acgcatctgt gcggcttttt actcctgaca tgg
//
LOCUS       DACAPB010000319         2258 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.327, whole genome shotgun sequence.
ACCESSION   DACAPB010000319 DACAPB010000000
VERSION     DACAPB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.327"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            352..2258
                     /locus_tag="IT904_004647"
                     /pseudo
     CDS             352..2258
                     /locus_tag="IT904_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439262.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          502 a          672 c          725 g          359 t
ORIGIN      
        1 taccggacaa aaacgcccgc gccggtgggg agcctcggcc ccggctggaa aatgcctgcg
       61 gatatccgct tacagctgcg cgataacaca ctgatactca gtgataacgg cggcagaagc
      121 ctgtattttg agcacctgtt tcccggtgag gacggttaca gccgcagcga gtcactgtgg
      181 ctggtgcgcg gcggcgtcct gagactggat gaaggtcacc ggctggccgc actctggcag
      241 gcgctgccgg aagaactccg cttaagtccg catcgttatc tggcgacaaa cagtccgcag
      301 gggccgtggt ggctgctcgg ctggtgtgag cgggtgccgg aagcggatga ggtgctgcct
      361 gcgccgctgc cgccgtaccg ggtactgacc gggctggtgg accgcttcgg gcgcacacag
      421 acgttccacc gcgaagccgc cggtgaattc agcggcgaaa tcaccggcgt gacggatggt
      481 gccgggcgtc acttccggct ggtactgacc acgcaggcgc gcgggcagaa gaagcctggc
      541 agcaggccat ttccggcggg acggaaccgt ccgcttttcc tgataccctg ccgggttaca
      601 ccgaatatgg ccgggacaac ggcatccgtc tgtctgccgt gtggctgacg cacgacccgg
      661 aatacccgga gaatttacct gccgcgccgc tggtgcgcta tggctggacg ccgcgcggcg
      721 aactggcggc agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata
      781 aataccgggg ccggatggtg gcgcaccgtc acacgggccg accggaaatc cgttaccgtt
      841 acgacagcga cgggcgggtg acagaacagc ttaacccggc aggcttaagc tacacgtatc
      901 agtatgagaa agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca
      961 cgcagggcga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
     1021 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
     1081 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
     1141 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
     1201 acgggctgga aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc
     1261 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
     1321 cggaagatgc caccggcagc cggaaaacca tgacgtggag ccgttacggt cagttgctga
     1381 gcttcaccga ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga
     1441 cggcggtgca ccgcgaggaa gggctgagtc agtaccgcgc atacgacagc cgtggacagt
     1501 taattgccgt gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg
     1561 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
     1621 gaaaggccgt ccgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
     1681 gacgggtcat ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
     1741 ttgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
     1801 tgaccggcaa acttatccgc agcgaggatg agggtctggt cacccactgg cactatgacg
     1861 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggtatg
     1921 acgaacgtgg ctggctgaca gacatcagcc atatcagcga agggcaccgg gtggcggtgt
     1981 attacggata tgatgagaaa ggccgcctga ccggtgagcg ccagacggtg catcacccgg
     2041 agacggaagc actgctctgg cagcatgaga ccagacatgc gtacaacgcg caggggctgg
     2101 cgaaccgctg tataccggac agcctgcccg ccgtggaatg gctgacctac ggcagcggct
     2161 ggctgtcagg catgaaactc ggcgacacac cgctggtgga gtacacccgc gaccgcctgc
     2221 accgggaaac gctgcgcagc ttcggccgtt atgaactc
//
LOCUS       DACAPB010000320         2233 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.328, whole genome shotgun sequence.
ACCESSION   DACAPB010000320 DACAPB010000000
VERSION     DACAPB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.328"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..600
                     /locus_tag="IT904_004648"
     CDS             1..600
                     /locus_tag="IT904_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP3194307.1"
                     /translation="MSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPW
                     WLVCRGTIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQ
                     NLYLDNLEATGLYQMPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKR
                     ERYTDKWQRRTHSLWRHQAWHASAFTGIYNDLAAASICV"
     gene            537..1169
                     /locus_tag="IT904_004649"
     CDS             537..1169
                     /locus_tag="IT904_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP3194308.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALAMQIPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            1230..>2233
                     /locus_tag="IT904_004650"
     CDS             1230..>2233
                     /locus_tag="IT904_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP3194309.1"
                     /translation="MGKGSSKGHTPREAKDNLKSSQMLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLVSVVVDNLPPRPFSIRMRRMTPDSTTDRLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQHCDQSVPDGFGGTEPRITCNAWLTTQRK
                     A"
BASE COUNT          470 a          576 c          725 g          462 t
ORIGIN      
        1 atgtcgccgg aagactggct gcaggcagag atgcagggtg agattgtggc gctggtccac
       61 agccaccccg gtggtctgcc ctggctgagt gaggccgacc ggcggctgca ggtgcagagt
      121 gatttgcctt ggtggctggt ctgccggggg acgattcata agttccgctg tgtgccgcat
      181 ctcaccgggc ggcgctttga gcacggtgtg acggactgtt acacgctgtt ccgggatgct
      241 taccatctgg cggggattga gatgccggat tttcatcgtg aggatgactg gtggcgtcac
      301 ggccagaatc tctatctgga taatctggag gccacagggc tgtatcagat gccgttgtca
      361 gcggcgcagc cgggcgatgt gctgctgtgc tgttttggtt catcggtgcc gaatcatgcc
      421 gccatttact gtggtgacgg tgagctgctg caccatattc ctgaacaact gagtaaacga
      481 gagaggtaca ccgacaaatg gcagcgacgc acacactccc tctggcgtca ccaggcatgg
      541 cacgcatctg cctttacggg gatttacaac gatttggccg ccgcatcgat ctgcgtgtga
      601 aaacgggggc cgaagccatc cgggcgctgg ccatgcagat cccggcgttt cgtcagaaac
      661 tgagcgacgg ctggtatcag gtacggattg ccgggcggga cgtcagcacg tccgggttaa
      721 cggcgcagtt acatgagact ctgcctgatg gcgctgtgat tcatattgtt cccagagtcg
      781 ccggggccaa gtcaggtggc gtattccaga ttgtcctggg agcagccgcc attgccggat
      841 cattctttac tgccggagcc acccttgcag cgtggggggc agccattggg gccggtggta
      901 tgaccggcat cctgttttct ctcggtgcca gtatggtgct cggtggtgtg gcccagatgc
      961 tggcaccgaa agccagaact ccccgcacac agacaacgga taacggtaag cagaacacct
     1021 atttctcctc actggataac atggttgccc agggcaatgt tctgcctgtt ctgtacggtg
     1081 aaatgcgcgt ggggtcgcgg gtggcttctc aggagatcag cacggcagac gagggggacg
     1141 gtggtcaggt tgtggtgatt ggtcgttgat gaaaaacgtt ttatgtgaaa ccgcctgcgg
     1201 gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc
     1261 cgcgcgaagc gaaggacaac ctgaaatcat cccagatgct gagcgtgata gacgccatca
     1321 gcgaagggcc gattgaaggt ccggtggacg gattaaaaag cgtgctgctg aacagtacgc
     1381 cggtgctgga cagtgagggg aataccaata tctccggtgt cacggtggtg ttccgggcag
     1441 gtgagcagga gcagacaccg ccggaggggt ttgaatcctc cggctccgag acggtgctgg
     1501 gtacggaagt gaaatatgac acgccgatca cccgcaccat cacgtcggca aacatcgacc
     1561 gactgcgttt tactttcggc gtgcaggcac tggtggaaac cacttcaaag ggggaccgga
     1621 atccgtcgga agttcgcctg ctggttcaga tacagcgtaa tggtggctgg gtgacggaaa
     1681 aagacatcac cattaagggt aaaaccacct cgcagtacct ggtctctgtg gtggtggata
     1741 acctgccgcc gcgcccgttc agtatccgga tgcgcaggat gacaccggac agcaccacag
     1801 accggttgca aaacaaaacg ctctggtcgt catacaccga aatcatcgat gtgaaacagt
     1861 gctacccgaa cacggcactg gtcggcgtac aggtggactc ggagcagttc ggcagccagc
     1921 aggtgagccg taattatcat cttcgcgggc gtattctgca ggtgccgtcg aactataacc
     1981 cgcagacgcg gcaatacagc ggtatctggg acggaacgtt taagccagca tacagcaaca
     2041 acatggcctg gtgtctgtgg gatatgctga cccatccgcg ctacggcatg gggaaacgtc
     2101 ttggtgcggc ggatgtggac aaatgggcgc tgtatgtcat cggccagcat tgcgatcagt
     2161 cggtgccgga cggttttggc ggcacggagc cgcgcatcac ctgtaatgcc tggctgacca
     2221 cacagcgtaa ggc
//
LOCUS       DACAPB010000321         2165 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.329, whole genome shotgun sequence.
ACCESSION   DACAPB010000321 DACAPB010000000
VERSION     DACAPB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.329"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(40..642)
                     /locus_tag="IT904_004651"
     CDS             complement(40..642)
                     /locus_tag="IT904_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194310.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(737..1015)
                     /locus_tag="IT904_004652"
     CDS             complement(737..1015)
                     /locus_tag="IT904_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAP3194311.1"
                     /translation="MPVKVARCFHDHTGWFNNIRRNILMATPVSLMDDQMVDMAFITQ
                     LTGLTDKWFYRLIRDGAFPAPIKLGRSSRWLKSEVEAWLQARIAQPRP"
     gene            1084..1350
                     /locus_tag="IT904_004653"
     CDS             1084..1350
                     /locus_tag="IT904_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194312.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            1651..1827
                     /locus_tag="IT904_004654"
     CDS             1651..1827
                     /locus_tag="IT904_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAP3194313.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
BASE COUNT          548 a          509 c          602 g          506 t
ORIGIN      
        1 atttccgtat tttgtcacac cttccggaag caaccggcat cagttgcggc tatcgtcaac
       61 cgttgtgcgg tatgccagga gcacgtactg atccatcaga tccgtcagtg ctgcgctctg
      121 cctctgacgg ttgatgatcc attcctccag ctctttttct gatttccgtt tcaggttctc
      181 tttccagtct tcaaggctgt ttccggtcat taaatcaagc cagcaaagcc agaccagttt
      241 gaggcgcaac tccgtgccac caaactgacg gaagaggctg atcagtttat ttgccagaga
      301 acgctgcctc agacgttcac attcagacag gagaatgcct tccagcatcg gatgatgaac
      361 cagcagatgt tcactggtgc ataaattcac tatcagatca tcatccaggg gcggcgtggc
      421 ccgtttgtca gggccataaa tcagccggtg caggctctgg ggtacgatgg caatctccgg
      481 tttttcacca ttgaatgaca cccaaccggg cgtcagatta tggaagtagt agatattcgg
      541 atgacacacg ccccaggata cggcgtgccg ggtgaagccg gtcagccatt tctggtagct
      601 ttcgtggagc cattcgtagc gggtctgata ctgttgtttc attttcatat tcctcacggg
      661 gatataacag gcagcagaat gccgccggca ggccggggat tacgtctgct gcgggtgaac
      721 ggataacggt cagaaattac gggcggggct gtgcaatacg cgcctgtagc caggcttcca
      781 cttcactttt cagccagcgg gagctgcggc ccagcttgat gggagccgga aaggccccat
      841 ccctgatgag cctgtaaaac cacttatctg tcaggccggt cagttgagtg ataaatgcca
      901 tgtcgaccat ctggtcatcc atcagtgaaa ctggggtagc catgagaatg ttcctccgga
      961 tgttgttaaa ccagccggtg tggtcatgaa aacagcgcgc caccttcacc ggcataacaa
     1021 ccggggagaa acgtaaaaaa taatcgggaa tgtgaccgcc aggagataaa cggggaggtt
     1081 gcggtggctg agccgtaccg gtataccggg tggttgttgc gaaaagatgc ttccgcacgc
     1141 acgcgcgata aagaggggat agttttcgtg aggacaggga gagaagcagg cgttctgata
     1201 attgcagagt tgttttcgga ggtggttaca gtgagtcagc ccgttcctgg cgaggaggag
     1261 actgatatca ccgatgggat aaggctgtct gattggtatt ataaatataa gaagtcatca
     1321 gagagtaacg taaaggattt gccagagtaa gctaagagag taaatctctc cggtaacaga
     1381 gcgctgtgaa tcgttcatgt atcagagtgc agatacatga acgctgatgc gtaaaatcag
     1441 cagtgacacg ccagctcccc ctgtgcccat tcctgtataa gacatgaact gagcgtatta
     1501 atgctcctgc cctgagactg agccggtgag tgaggggctg agaaatccct cttgacactg
     1561 ctctaaacca gaaatacttc cccggttgtt gcggtgcgtc cggatccccg ataacggaac
     1621 actaaggctg tattgcggga caccaggtaa gtggcagccc gtaatacgtc aggagacgcg
     1681 ggaaaagtga attcccacac gcgttataaa cttacccttg cggagctgga agcctttaac
     1741 tctgccgttg acaaccggct ggcagaactg acaatgaaca aactttacga tcgcgcgccg
     1801 gcttccgtct ggaaatatgt cacctgaata ttgaagcagc gctacgatga ctccggtgtt
     1861 atctgcaccg gaggaccggg aacattaagc ggtatagcga caacaaagaa ttcgaaatca
     1921 aaagggaatg gtgccggagg cgtgggcggt aataggctga ttttcaggta ttaccggctt
     1981 acaacgtgtg gttgatattg aggggctgtg agtttcgcgt atttgtcaga aacccagatg
     2041 agttttcctg tatgggacga aataaggcag cggcaatacc tggaaaattt aatatggtat
     2101 gaaggagaac ctgtcggtta cgcttaatat acacggtaca ctgtcagctc aggggataca
     2161 tgaac
//
LOCUS       DACAPB010000322         2155 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.330, whole genome shotgun sequence.
ACCESSION   DACAPB010000322 DACAPB010000000
VERSION     DACAPB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.330"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..373)
                     /locus_tag="IT904_004655"
     CDS             complement(<1..373)
                     /locus_tag="IT904_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="periplasmic sensory protein associated with the
                     TorRS two-component regulatory system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAP3194314.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVAS"
     gene            456..>2155
                     /gene="torS"
                     /locus_tag="IT904_004656"
     CDS             456..>2155
                     /gene="torS"
                     /locus_tag="IT904_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAP3194315.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     T"
BASE COUNT          546 a          563 c          587 g          459 t
ORIGIN      
        1 gacttgctac ctgcttttgc aggtcgggaa atgaggtcgt gctactaccg agcaaaatgg
       61 cctctgcgcc ccactgttta cactggtcga tttgtgcttg ctgggtagcc aactggctgt
      121 agccgcctgc ctccagcact tttaaatcca caccgtagcg gcgagcagcc tcctgcatac
      181 catagttcaa cgataaccaa tatgaatctt tcaggctggg ataaagcgcg cacagtttcc
      241 atgcgcgttt ggctttaagc ggcgtagagg cttgcaccgt gaaatgctgc gcatcatgcc
      301 agcgcaacag gttatcagcc gaaaatgccg gcaacatgaa aagggaaaga agtaaaaata
      361 gcagtacgcg catgatagcc tcatcaataa taaggcttta tgctagatgc attccgcttt
      421 gcgactcaac ctttttcacc taaagtgcac cgaccgtgaa tttaaccctg acccgaagac
      481 tctggatggg ctttgccctg atggcgctgt taaccctgac cagtaccctg gtgggatggt
      541 acaacctgcg ctttatcagc caggtggaaa aagacaacac tcaggcattg attcctacca
      601 tgaatatggc gcgccagttg agcgaagcca gcgcctggga acttttcgcc gcgcagaacc
      661 tgaccagtgc cgataacgaa aagatgtggc aggcgcaggg gcgaatgctc accgcacaaa
      721 gcctgaagat taatgcgttg ctgcaagcgt tacgggaaca aggttttgat accaccgcta
      781 ttgaacaaca ggagcaggag atctcccgtt cgttacgtca gcaaggggaa ctggtggggc
      841 agcgtttgca actgcgccag caacaacagc aactcagtca gcagatagtc gctgccgccg
      901 atgagatcgc acgcctggcg caaggtcagg cgaataatgc gacaacttcc gctggagcga
      961 cccaggccgg gatttacgat ttgatcgaac aagatcagcg tcaggctgct gaaagtgcac
     1021 tcgatcggct gattgatatc gatcttgagt atgttaacca gatgaatgaa ctgcgcctta
     1081 gcgctctgcg ggtgcagcaa atggtgatga atctggggct ggagcagatc cagaaaaatg
     1141 caccaacgct ggaaaagcag ctcaataatg cggtgaaaat tctgcaacgt cggcaaatac
     1201 gcattgaaga tccgggtgtt cgtgcgcagg tcgcaacaac gttaactacc gttagccaat
     1261 atagcgattt gctggcgctg tatcagcagg acagtgaaat cagcaatcac ctacaaactc
     1321 tcgcacaaaa taacatcgcc cagttcgcgc agtttagtag cgaagtcagt cagctggtcg
     1381 acaccattga gctgcgtaat cagcacggac tggcgcatct ggaaaaagcg agtgcacgcg
     1441 ggcaatacag cctgttattg ctggggatgg tttcactttg cgcactgatt ctgatcctct
     1501 ggcgcgtggt ttatcgctca gtcacgcgtc cacttgccga acaaacgcag gcgctgcaac
     1561 ggctgctgga cggtgatatt gactcccctt tcccggaaac cgctggcgta cgggagctgg
     1621 ataccatcgg gcggctgatg gatgcgtttc gcagcaatgt tcatgcactg aatcgccacc
     1681 gcgaacagct ggcggcgcag gtcaaagcgc gtacagctga attgcaggaa ctggtgatag
     1741 aacaccgaca agcacgggcg gaagcagaaa aagccagcca ggcaaaatcg gcgtttctgg
     1801 cggcgatgag ccatgagatc cgcacaccgc tgtacggtat tctcggcact gctcaactgc
     1861 tggcagataa ccccgcactt aacgcccagc gtgatgattt gcgggcaatt actgactctg
     1921 gtgaatcgtt gctgaccatc ctcaacgata ttctcgatta ttccgctatc gaagcaggtg
     1981 gcaagaatgt ttcggtcagc gatgagccct ttgaaccgcg cccgctgctg gaaagtaccc
     2041 tgcaattaat gagcggacgg gtgaaaggtc gcccgattcg cctggcaacg gcaattgccg
     2101 atgatatgcc gtgcgcgtta atgggcgatc cacgacgtat tcgtcaggtt ataac
//
LOCUS       DACAPB010000323         2154 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.331, whole genome shotgun sequence.
ACCESSION   DACAPB010000323 DACAPB010000000
VERSION     DACAPB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.331"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..2074
                     /locus_tag="IT904_004657"
     CDS             <1..2074
                     /locus_tag="IT904_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAP3194316.1"
                     /translation="HDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDD
                     HVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEG
                     GGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVME
                     FVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPM
                     AYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKL
                     SLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVF
                     MDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPAN
                     GQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGA
                     TQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGL
                     AEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIG
                     SMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPT
                     FEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLI
                     NFNKNRQM"
BASE COUNT          519 a          525 c          608 g          502 t
ORIGIN      
        1 tcacgatatt acctttgttg gtattgtaca gaccgctaaa gcgtccggaa tggaacgttt
       61 cccgctgccg tatgtgctga ccaactgcca taactcactc tgcgccgtcg gcggcaccat
      121 taacggtgat gaccatgttt ttggtttatc ggcagctcag cgttatggcg gtatttttgt
      181 gcctccgcat attgcggtca tccatcaata tatgcgtgag atgatggcag gcggcggcaa
      241 aatgatcctc gggtcagaca gccacacccg ttacggtgca ttagggacaa tggcagtcgg
      301 tgagggcggc ggtgagttgg taaaacagct gcttaatgac acctgggata tcgactatcc
      361 gggagttgtt gcggtgcatc tgaccggaaa accagcgccg tatgtggggc cgcaggatgt
      421 ggcgctggct atcatcggtg ccgtgttcaa aaacggctac gtcaaaaaca aagtgatgga
      481 attcgtaggt cccggtgttg ctgcgctctc taccgatttc cgtaacagcg ttgacgttat
      541 gaccactgaa acgacctgtt taagttctgt ctggcaaacc gatgaagaag tccataactg
      601 gctggcgctg cacggtcgcg gccaggatta ctgccagctt aaccctcaac cgatggcgta
      661 ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt
      721 ccacccgagc aacgtgtatg aaatcgacac actgaaccag aacctgaccg acattctgcg
      781 tgagattgaa attgagtccg aacgcgtggc gcacggtaaa gccaaactct cgctgctgga
      841 taaagtggaa aatggtcgcc tgaaagtgca gcaggggatt atcgcgggct gttctggcgg
      901 taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga
      961 caccttctcg ctggcagttt acccgtcatc acagccggtg tttatggatc tcgccaaaaa
     1021 aggtgtggta gcagatttga ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc
     1081 atgctttggc gcgggcgata cgccaatcaa caacggatta agtattcgcc acaccacgcg
     1141 caacttcccg aaccgcgaag gctctaagcc agctaatggg cagatgtcag cggtggcgtt
     1201 gatggacgct cgttctatcg ctgcgactgc ggcaaacggt ggctatttaa cctctgccag
     1261 cgaacttgat tgctgggaca acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa
     1321 ccgtgtttat cagggctttg tgaaaggggc aactcagcaa ccgctgattt acggaccgaa
     1381 cattaaagac tggccggaat tgggtgcgct gactgacaat atcgtcctga aagtgtgctc
     1441 gaagatcctc gacgaagtga ccaccaccga cgaactgatt ccttccggtg aaacctcttc
     1501 ttatcgttca aatccgattg gtctggcgga gtttaccctg tcacgccgcg atcccggtta
     1561 tgttggcaga agtaaagcga ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag
     1621 cgagctgaca gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc atattgatcc
     1681 gctgcaaact gaaattggca gcatggtata tgcggtgaaa ccaggcgatg gttctgcgcg
     1741 tgaacaggcg gcgagctgcc agcgtgtgat tggcggtctg gcgaatattg ccgaagagta
     1801 cgcgactaaa cgctaccgtt ctaacgtcat caactggggg atgttaccgc tgcagatggc
     1861 ggaagtgcca acctttgaag tgggggatta catttacatc cctggcatta aagcggcgct
     1921 ggataatccg ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga
     1981 aattacgctc tatatggaaa gtctgactgc tgaagagcgc gagattatca aggcgggtag
     2041 tttgattaac ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca
     2101 ttcggcactt attgtcggat gcgatgcttg cgcatcttat ccgacctacg aatc
//
LOCUS       DACAPB010000324         2149 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.332, whole genome shotgun sequence.
ACCESSION   DACAPB010000324 DACAPB010000000
VERSION     DACAPB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.332"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            37..1683
                     /gene="cka"
                     /locus_tag="IT904_004658"
     CDS             37..1683
                     /gene="cka"
                     /locus_tag="IT904_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HAP3194317.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKL
                     NSYKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELA
                     KAESDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAV
                     ENELKNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEK
                     YAKIAQDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDEL
                     SKNLTKISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVAL
                     MFSFIVGVPLGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
     gene            complement(1680..1970)
                     /gene="cki"
                     /locus_tag="IT904_004659"
     CDS             complement(1680..1970)
                     /gene="cki"
                     /locus_tag="IT904_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HAP3194318.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            2000..2131
                     /locus_tag="IT904_004660"
     CDS             2000..2131
                     /locus_tag="IT904_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HAP3194319.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
BASE COUNT          725 a          277 c          514 g          633 t
ORIGIN      
        1 ttttaattta ttgttttaat tatcaaagag gaatttatgg ctaaagaact aagtggatat
       61 ggaccaactg ctggtgagtc tatgggggga acaggggcga atctgaatca gcaaggtgga
      121 aataataata gtaatagtgg ggttcactgg ggtggtggct ctgggcatgg taataatggt
      181 ggtcagggaa attcaaattc atctggttct acatcaactg tgatgaaaac cggtgagtca
      241 tatctgactc cgtggggtga tgttgtcatt aataatgatg gccttccggt gatgaatggt
      301 attgttatga cagaagagaa ttcaactctg gttgataatc catttggagg ggtctccaga
      361 gttcttaatt ctttaattag tgatatgcca tcactttttg cagaaagctc aggaaataac
      421 aacaataaca ctgcttcagt taatactgca ccaacgaatg ctcaggtcag tgatatggat
      481 aagagttcga aggtggtggg taatgtaatt aatgaaaaac aaaagcagaa gaataaaatt
      541 gctacacaga ttagtgaaaa acagaagaaa atcgaggaga tgaaaaaagt attcaagcat
      601 cattcttatc atggtatcac tgatcttgag agggatgttg atgagttgca aaaaaaatca
      661 aatcagcttg atgcagatat tagcaaactt aattcttata aaaatacatt gcaaagtaaa
      721 attggtgatg tgaataaaca gaaggaagct gaagaaaagg ctcgtgaaaa tgcagaggtc
      781 gctgaacatg aaactcttaa tgaagagaaa caggcggtgg cggaagcaga gaagcgttta
      841 gctgaggcga aagctgaact ggcgaaggct gaaagtgatg tacagagtaa gcaagctact
      901 gtttccagag ttgcaggaga gcttgaaaac gctcagaaga gtgttgacgt gaaggtcacc
      961 ggatttccgg gatggcgtga cgttcagaaa aaactacagc gtcagcttga agctaagcag
     1021 gctgagtact ccgcagttga aaatgaactt aagaatgctg ttagtttcag ggatggtaag
     1081 gccgctgagg ttaaagaggc agagcagaaa ttaaaagaag ctcaggatgc tcttgaaaag
     1141 agccagataa aagatgctgt tgatactatg gttggatttt atcaatatat aaccgaacaa
     1201 tatggagaaa aatacgcaaa aattgctcag gatttagcgg aaaagtctaa aggaaagaaa
     1261 attcaaggtg ttgatgaagc tctggcagca tttgaaaagt ataagaatgt actggataag
     1321 aaattcagta aggttgatcg ggatgctatt tttaatgcgt tagagtctgt taattatgat
     1381 gagttatcta agaatttaac gaagatatca aagtcgctta aaatcaccag tcgagtatct
     1441 tttctatatg atgttggttc tgattttaaa aatgcaatag aaactggtaa ctggcgacct
     1501 ttgtttgtca cgctggagaa aagtgcggtc gatgtaggtg tggcaaaaat tgtggcatta
     1561 atgtttagtt ttattgttgg tgtgcctctt ggtttctggg ggattgcaat tgtcacaggt
     1621 attgtttctt cttacatagg ggatgatgag ttgagcaaac ttaatgagtt gttagggatt
     1681 taatttctct ttagagtgta atatatgaaa cttggcaatc ctactgggat tgccattaat
     1741 aaataaaaca atgcagataa cttgcataag taccagggtt ctttttttaa ctttgtattt
     1801 tctttgatat atctagatat ggttaattta gagtatggat atagcaatac atgtattgat
     1861 gatataaata taaataacaa aggagtgtta tcattgtcgt taaatattaa aataagaatc
     1921 catggtataa gtgattcagg taaattatgt aggtagtatt ttaaatgcat aagtattttt
     1981 acttttgttg gaaagaaata tgaaaataat aatttttcta attacaatat tattcttgtc
     2041 aggatgtcaa gcaaactata ttcgtgatgt acaaggagga accgttgcac catcttcgtc
     2101 ttctgaactg acggggatcg cggttcagta gaaaagatca aaggatctt
//
LOCUS       DACAPB010000325         2137 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.333, whole genome shotgun sequence.
ACCESSION   DACAPB010000325 DACAPB010000000
VERSION     DACAPB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.333"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..227)
                     /locus_tag="IT904_004661"
     CDS             complement(<1..227)
                     /locus_tag="IT904_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAP3194320.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMAT"
     gene            complement(513..1238)
                     /gene="phoU"
                     /locus_tag="IT904_004662"
     CDS             complement(513..1238)
                     /gene="phoU"
                     /locus_tag="IT904_004662"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP3194321.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1253..2026)
                     /gene="pstB"
                     /locus_tag="IT904_004663"
     CDS             complement(1253..2026)
                     /gene="pstB"
                     /locus_tag="IT904_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAP3194322.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
BASE COUNT          516 a          549 c          494 g          578 t
ORIGIN      
        1 caggttgcca tcacctcaag aggaatatga cttaagagtt cgcttaatcg cccgttcagt
       61 tcatgactgc tcaaggcata ctctttttct attccacttg agttaattct ttcgccagcg
      121 cgtttttgaa agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata
      181 accaccacaa cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt
      241 tttataaaca aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc
      301 gaatgcagta acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga
      361 aattaattta attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg
      421 ctcgcagtta ttaactttgt gtaattttag gaatttataa agttatatat aacaaatccc
      481 aataattaag ttattgggat ttgtctggtg aattatttgt cgctatcttt ccccgccagc
      541 agtttatcca gctcatcgcc accgacgtga cggaaatcct gccccttcac gtagtagaag
      601 ataaactcac aaatattctg gcagcggtcg ccaatacgtt cgatagaacg cgcgcagaac
      661 agcgcagtaa gtacgctcgg aatggtacgc gaatcttcca tcatgtaggt catcagttga
      721 cgaacaatac cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct
      781 tcgtcaatgt ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga
      841 cggcccagcg actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc
      901 gcagtacggc agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta
      961 ctgatcacca taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc
     1021 acgcaggctt catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatgacg
     1081 cgcttcgcca gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc
     1141 tccaccatgc cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac
     1201 tggccggaaa tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt
     1261 aacgaccggt gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg
     1321 tgttgctgaa ctcaatcaat tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg
     1381 cagcctgctg catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga
     1441 tcagctcttc aatacgcccg gtagagatag ggtcgagcgc cgaacacggt tcgtcgagca
     1501 gcagcacttc cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac
     1561 cagagagaga gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt
     1621 tggtcaatgc ccactgcacg cgctcgtcca tatcggcacg ggagagcttc tcaaacagac
     1681 gaacgccaaa agcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca
     1741 ccatgcccac tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc
     1801 catcaagcag aatttcacct tccgcacgct gctccgggta cagttcaaac attttgttga
     1861 aggtacgcag cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gtaacctggt
     1921 ttttggcgat atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca
     1981 aattacgaac ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct
     2041 catcgtgcct gatgcacttc gctaatcagg cttaccgtag gccggatcag gcgttcacgc
     2101 cgcatccggc aaagtgttaa ccgtgtacag agtcaaa
//
LOCUS       DACAPB010000326         2089 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.334, whole genome shotgun sequence.
ACCESSION   DACAPB010000326 DACAPB010000000
VERSION     DACAPB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.334"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1723
                     /gene="mngA"
                     /locus_tag="IT904_004664"
     CDS             <1..1723
                     /gene="mngA"
                     /locus_tag="IT904_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAP3194323.1"
                     /translation="PHGKTAAVKEAAFEVATLSEPLQWEGVDGPEAVDLVVLLAIPPN
                     EAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASFSNAPTIV
                     CVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGTNGIEGRLTADQLNSATACIFA
                     AEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDTQPVKSVK
                     TELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMYRKLGGGL
                     LGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAGYLMRWVK
                     NHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNGLSGSNAL
                     LLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFTVTASTML
                     APRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTGAIVGAMN
                     IGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRHAVKHGNY
                     LTDGVMP"
     gene            1741..>2089
                     /locus_tag="IT904_004665"
     CDS             1741..>2089
                     /locus_tag="IT904_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194324.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFG"
BASE COUNT          456 a          514 c          612 g          507 t
ORIGIN      
        1 gccgcatggc aaaactgctg cggtaaaaga agcggcgttt gaggtcgcca cactcagcga
       61 gccgcttcag tgggaaggcg ttgatggccc ggaagcagtt gatttagtgg tgctgctggc
      121 gattcccccc aatgaagcgg gtactacgca tatgcaactg ctgacagcgc tgaccacgcg
      181 ccttgcggat gatgagattc gggcgcgtat acagtcggcg acgacgcctg atgagttgct
      241 ctcggcgctg gatgacaagg gaggcacgca accttctgcc tctttttcca acgcgccaac
      301 tatcgtctgc gtaacggcct gtccggcggg tattgctcac acctatatgg ctgcggaata
      361 tctggaaaaa gccggacgca aactcggcgt aaatgtttac gttgaaaaac aaggcactaa
      421 cggcattgaa gggcgtttaa cggcggatca actcaatagt gcaaccgcct gtatttttgc
      481 ggctgaagtc gccatcaagg agagtgagcg ttttaatggc attcccgcgc tttcagtgcc
      541 tgttgccgag ccgattcgcc atgcagaagc gttgatccaa caagcgctta ccctcaagcg
      601 tagcgatgag acgcgtaccg tacagcaaga tacgcaaccg gtgaaaagtg tcaaaacgga
      661 gctgaaacag gcactgttga gcggaatctc ttttgccgta ccgttgattg tcgcgggggg
      721 cacggtgctg gcggtcgcgg tattactgtc gcaaatcttc gggctacaag atctgtttaa
      781 tgaagaaaac tcctggctgt ggatgtaccg caagctgggc ggcgggctgc tcggaatttt
      841 gatggtaccg gtgctcgcgg cctataccgc ctattctctg gcagataaac cggcgttagc
      901 gccaggcttt gcggctggac ttgccgccaa catgatcggc tccgggtttc tcggcgcggt
      961 cgttggcgga ttgatagccg gttacttgat gcgctgggtg aaaaatcact tgcgtcttag
     1021 cagtaaattc aatggattcc tgacttttta tctctacccg gtgctcggta cgttgggagc
     1081 gggcagtctg atgctgtttg tggtggggga acctgtcgcc tggatcaata actcgcttac
     1141 cgcctggctg aacggtctgt caggaagtaa cgcgctgttg ctgggtgcca ttctcggttt
     1201 tatgtgttcc tttgaccttg gagggccagt gaataaagcc gcttatgcat tctgcctggg
     1261 cgcaatggcg aacggcgttt acggcccgta tgccattttc gcctccgtca aaatggtttc
     1321 ggcatttacc gtaaccgctt ccacgatgct cgcaccgcgc ctgtttaaag agtttgaaat
     1381 tgagaccggg aaatccacct ggctgttagg gctggcaggt attaccgaag gggcgatccc
     1441 gatggcgatt gaagatccgc tgcgggttat tggttcgttt gtgctgggct ctatggtaac
     1501 gggcgctatt gtcggtgcga tgaatatcgg cctttcgaca cccggtgccg gcattttctc
     1561 gctcttttta cttcatgata atggcgcggg cggtgttatg gcggcaattg gctggtttgg
     1621 cgcggcattg gtgggggctg caatctcgac tgcaattctc ctgatgtggc ggcgtcacgc
     1681 ggttaagcat ggcaactatc tgactgatgg cgtaatgcca taaacaaaaa ggaaacgacg
     1741 atgaaagcag tatctcgcgt tcacatcacc ccgcatatgc actgggatcg agagtggtat
     1801 ttcaccaccg aagagtcacg tattctgctg gtcaataata tggaagagat cctgtgccga
     1861 ctggaacagg acaacgaata caaatattac gtactcgacg ggcaaacggc gatcctcgaa
     1921 gattatttcg cggtgaaacc ggaaaacaaa gaccgtgtga agaaacaggt agaagccggc
     1981 aagttgatta tcggcccctg gtatacccag accgatacca cgattgtttc tgcggaatcc
     2041 atcgtccgta atctgatgta cggaatgcgt gactgcctcg cgtttggcg
//
LOCUS       DACAPB010000327         2073 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.336, whole genome shotgun sequence.
ACCESSION   DACAPB010000327 DACAPB010000000
VERSION     DACAPB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.336"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(93..635)
                     /locus_tag="IT904_004666"
     CDS             complement(93..635)
                     /locus_tag="IT904_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAP3194325.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAKHPGLICILRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKIA"
     gene            complement(632..922)
                     /locus_tag="IT904_004667"
     CDS             complement(632..922)
                     /locus_tag="IT904_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAP3194326.1"
                     /translation="MVDLRKAAKGQMCQIRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIAYSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(922..1521)
                     /locus_tag="IT904_004668"
     CDS             complement(922..1521)
                     /locus_tag="IT904_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAP3194327.1"
                     /translation="MAHIQLIKHTSSGLLLPATSESCEFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITSRERKLVSGFVDYLCDSVGREHTPALSDAAEQ
                     YLNTIAIRRTCDTALLKSFDAFREWVTIEAGFYTEHFYPDGSRGRRARSIAFANMDDT
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1588..1848)
                     /locus_tag="IT904_004669"
     CDS             complement(1588..1848)
                     /locus_tag="IT904_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194328.1"
                     /translation="MDFKKLRKIFNKDGLYAVRVENGEIISCYCIHCSRALQQPSGAM
                     LIYLLNGHVTDGFMLREDEFVTSLRTLKEIGLKAGFSAFEDD"
BASE COUNT          544 a          531 c          434 g          564 t
ORIGIN      
        1 tggcgggttc tggtaaagtt catgcgcttg gttcgcctcg cgatacagct ttgcgaaaca
       61 taccggaatt gaagcaattt atgcgtaaaa aatcaagcta ttttttgagc aaatgattct
      121 cgcatgggaa tatacagggc atattcagca acggctaacc aattagcaat tcgcttttcg
      181 catgtgctaa aacaccactc aggatgtgca tcattcagca attcagccat tttacgctta
      241 gtcatccccc tcccttcata tcgttgccgg aggatacaaa tcaatcctgg gtgttttgcc
      301 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      361 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      421 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      481 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      541 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      601 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      661 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      721 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct ataggcaatc gctgcctgca
      781 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
      841 aggtttcggg attgtgattg cagtagccag ggattctgat ctggcacatc tggcctttag
      901 ccgctttacg taaatccacc attacgcaaa ctccagcagc tgcgcagcaa cattttcgac
      961 ttgttccgga gaggaaaatt tacggaacag gatccagttc caaagcacat tcagtacgga
     1021 tttataaacc tgctgaaact cggtatcgtc catgttcgca aacgcgatag atcttgccct
     1081 gcgtccacgg ctaccgtccg gataaaaatg ctcggtgtaa aatccggcct caatagttac
     1141 ccattcacgg aaagcatcaa atgactttag caatgccgta tcacaggttc tgcgtatcgc
     1201 aatggtattc agatattgtt ctgcggcatc actcagggct ggcgtatgtt cccggccgac
     1261 cgagtcacac agataatcaa cgaaaccgga cacaagtttt cgttcacgag aggtgatcgc
     1321 cccaccgaca ggagtccagt aatcgaatcc gagttgcagg agtttgaaaa aacgcttgtg
     1381 aaatgcgtag ttacgaacgc gcttaaaatc agcgtgtatc cactcaccta tttttatttg
     1441 atgcaagaat tcgcaactct ccgatgttgc cgggagcagt aatccagaag aggtatgttt
     1501 gatcagttgt atatgtgcca tcggctttct ccggtggcac ggtgttactc agcagggggt
     1561 caaccctgcg ctgaattgta gatgagttca atcgtcttca aaagcagaaa agccagcttt
     1621 taatccaatt tcttttaatg tccgtaatga tgtgacaaat tcatcttcac gcaacataaa
     1681 tccgtccgtc acatgcccat tcaagagata tattaacata gccccactag gctgttgcaa
     1741 agcccgtgag caatgaatgc agtaacaact aataatttcc ccattttcaa cgcgcacagc
     1801 atagagacca tctttattaa aaattttgcg caacttcttg aaatccataa acagaaattt
     1861 tccagattaa tagcgttccc ctgaagggtc catccctctt ctccctgcgc gctaactaag
     1921 caaacacgat tctatttaca acagtcagtc cctcaagact tacggctaaa agagtacaca
     1981 aaaagctaat ttatccacca caacaaaaaa cccgccgaag cgggtaagta cgggtgcgtt
     2041 gaggatgcct gacacatcag aggtggcggg gga
//
LOCUS       DACAPB010000328         2056 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.337, whole genome shotgun sequence.
ACCESSION   DACAPB010000328 DACAPB010000000
VERSION     DACAPB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.337"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..357
                     /locus_tag="IT904_004670"
     CDS             <1..357
                     /locus_tag="IT904_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP3194329.1"
                     /translation="VTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPM
                     VEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRF
                     TLLRRGKKNYCLICWK"
     gene            419..1279
                     /gene="pdxY"
                     /locus_tag="IT904_004671"
     CDS             419..1279
                     /gene="pdxY"
                     /locus_tag="IT904_004671"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAP3194330.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(1323..1928)
                     /gene="gstA"
                     /locus_tag="IT904_004672"
     CDS             complement(1323..1928)
                     /gene="gstA"
                     /locus_tag="IT904_004672"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAP3194331.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
BASE COUNT          500 a          496 c          549 g          511 t
ORIGIN      
        1 gtgactcgtc tggttcacgg tgaagaaggt ttacaggcgg caaaacgtat taccgaatgc
       61 ctgttcagcg gttctttgag tgcgctgagt gaagcggact tcgaacagct ggcgcaggac
      121 ggcgtaccga tggttgagat ggaaaagggc gcagacctga tgcaggcact ggtcgattct
      181 gaactgcaac cttcccgtgg tcaggcacgt aaaactatcg cctccaatgc catcaccatt
      241 aacggtgaaa aacagtccga tcctgaatac ttctttaaag aagaagatcg tctgtttggt
      301 cgttttacct tactgcgtcg cggtaaaaag aattactgtc tgatttgctg gaaataatgc
      361 attaagtgga aagggggagt gagaaatcac tccccctggt ttttatacag ggaacatgat
      421 gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt catgcgggta acagtgcggc
      481 agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg ctgaacaccg ttcaattttc
      541 taatcacacc caatacggca aatggactgg ctgcgtgatg ccgcccagcc atttaaccga
      601 aattgtgcaa ggcattgccg ccattgataa attacacacc tgtgatgccg tattaagtgg
      661 ctatctggga tcggcggagc agggtgaaca tatcctcggt atcgtccgtc aggtgaaagc
      721 cgcgaatccg caggcgaaat atttttgcga tccggtaatg ggtcatccgg aaaaaggctg
      781 tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac ggtttgcctg ccagcgatat
      841 cattgcgcca aatctggttg agctggaaat actctgtgag catgcggtaa ataacgtcga
      901 agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg ccacaaattg tgttggttaa
      961 acacctggcg cgagctggct acagccgtga ccgttttgaa atgctgctgg tcaccgccga
     1021 tgaagcctgg catatcagcc gtccgctggt ggattttggt atgcgccagc cggtaggtgt
     1081 tggtgatgtg acgagcggtt tactgctggt gaaactgctt cagggggcaa cgctgcagga
     1141 ggcgctggaa catgtgaccg ctgcagtcta cgaaatcatg gtgaccacca aagcaatgca
     1201 ggaatatgag ctgcaagtgg tggctgctca ggatcgtatt gccaaaccag aacattactt
     1261 cagcgcaaca aagctctgaa atatctcatt tcggcccaca acgacctgtg ggccgatggc
     1321 tattacttta agccttccgc tgacagcgcg tcttgtactt ccggacgttc agccatacgt
     1381 tgcataaatg ctgcaatgtg ctctaaccct tccagattca gtttcaccgc gtatgcccag
     1441 cgcagaaccg taaacagata ggcatcagca attgtaaatc tttgcccgca gatccagtgc
     1501 tcatccttca gtgcctcgtt cacatattgc agcttcttct ccagctgcgc gcgaactgtc
     1561 ggtttgtact cttccggtgt atcagggcga aacagaggtg tgaaaccttt atgcagctcg
     1621 gtggcgatgt aattcagcca ttcgatggtt ttatagcggg aaatactgtt taccggtgcc
     1681 agcaactggc ggtcggggac gctgtcggca agatactgca taatcgctac gccttccgtc
     1741 agcaaagtac cgtcatccag cagcaatgca ggcacctgcc ccttagggtt aacggcaaag
     1801 taatcgtcac cgttttcgag acgttttttc attaaatcca cactgacgag ggtaaaatcc
     1861 tttccgctct cacgcagggt gatatgggaa gcgagagagc aggcacccgg tttgtagaac
     1921 aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt tgctgaacaa
     1981 aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga gtttccctga attttacgct
     2041 acggctgctt tcgccg
//
LOCUS       DACAPB010000329         2026 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.338, whole genome shotgun sequence.
ACCESSION   DACAPB010000329 DACAPB010000000
VERSION     DACAPB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.338"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..462
                     /gene="ybiV"
                     /locus_tag="IT904_004673"
     CDS             <1..462
                     /gene="ybiV"
                     /locus_tag="IT904_004673"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAP3194332.1"
                     /translation="PEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPLVI
                     DKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGNDA
                     EMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            614..1879
                     /locus_tag="IT904_004674"
     CDS             614..1879
                     /locus_tag="IT904_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAP3194333.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT          508 a          521 c          522 g          475 t
ORIGIN      
        1 cccgaagcat tcgtcgcact gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat
       61 tatcaggaga ttgacgacgt actgttcaag ttttcgctca acctgccgga tgaacaaatc
      121 ccgttagtga tcgacaaact gcacgtagcg ctcgatggca ttatgaaacc cgtcaccagt
      181 ggttttggct ttatcgacct gattattccc ggtctacata aagcaaacgg tatttcgcgg
      241 ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga cagcggtaac
      301 gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac
      361 attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc gctgaatgtg
      421 attcaggctg tgctggataa cacatcccct tttaacagct gacctctcta ccggagccat
      481 gcgctccggt tttcccttct ttttacatcc tgtacatcac atcaaatcac taaacttgca
      541 ttaacttatt tataacttat attgtcacat gcaaatcatt atagtttcga atgaaagtta
      601 atgcgaggtg gttatggctt ctacttttac cagcgacaca ttgcctgccg atcacaaagc
      661 agctatccgt cagatgaagc acgcgctgcg ggcgcagctt ggcgacgtcc agcagatctt
      721 taatcagcta agcgatgaca ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca
      781 gggcgatgcc gtctggccgg tgctgtcata tgccgatatc aaagcaggtc atgttactgc
      841 agagcagcgc gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg
      901 cgaacaagcg ctaggctggg atcagtcgat gctggactat ctggaccgca accgctttga
      961 cgaggtctac aaaggccccg gcgataattt cttcgggacg ctcagcgctt cacgtcccga
     1021 gatttacccc atctactggt cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc
     1081 gaatgcgcag tcgtttctca atcgtctgtg gacatttgaa agtgatggaa aacaatggtt
     1141 taacccggat gtgagcgtca tctaccctga ccgtatccgc cgccgtccgc ccggaacgac
     1201 ctccaaaggt cttggagcgc ataccgactc cggggcactg gaacgctggc tgcttccagc
     1261 gtatcagcac gttttcgcca acgtctttaa tggcaatctg gcgaaatacg atccctggca
     1321 tgcggcacat cgtacggaag ttgaagagta cacggtggac aacaccacca aatgttccgt
     1381 gtttcggaca ttccagggct ggacagcgct ctctgatatg ctacctggtc agggactgct
     1441 gcacgtcgtg cccattcctg aagccatggc gtacgtactg ttacgtccgc tgcttgatga
     1501 tgtgccggag gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg tatcagagca
     1561 atggcatcca ctgttaattg aggcgttaac cagcattcca aaactcgagg ccggagactc
     1621 cgtctggtgg cactgcgacg tcatccattc cgttgccccc gttgaaaatc aacagggctg
     1681 gggcaacgtg atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca
     1741 caaggtgaag gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta
     1801 tgaaacaaac tgggaaggac gctttacgct tgccgacctc aacattcacg gtaagcgagc
     1861 gttgggcatg gacgtttgag agttaatgga agtgaattga tgtgggatta agaaaatcga
     1921 ttcgacgtta ctcagattgc tgacaagtgc gctttcttaa tgcctgatgc ggcgttaacg
     1981 tcttatctgg cctacaaagt cctgcagatt caatatatta caggca
//
LOCUS       DACAPB010000330         2026 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.339, whole genome shotgun sequence.
ACCESSION   DACAPB010000330 DACAPB010000000
VERSION     DACAPB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.339"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(14..1198)
                     /gene="araJ"
                     /locus_tag="IT904_004675"
     CDS             complement(14..1198)
                     /gene="araJ"
                     /locus_tag="IT904_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAP3194334.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(1324..>2026)
                     /locus_tag="IT904_004676"
     CDS             complement(1324..>2026)
                     /locus_tag="IT904_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414931.1"
                     /note="with SbcD cleaves DNA hairpin structures; also has
                     5' single-strand endonuclease activity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAP3194335.1"
                     /translation="PDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDADN
                     RQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQLTR
                     LHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSHKT
                     RIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVKKI
                     NGLGYSKLESTFAVK"
BASE COUNT          547 a          571 c          462 g          446 t
ORIGIN      
        1 ccgtgacagt aatctacccc agtggtttcg ccagcaccgg agtatccgcc gcttgctggc
       61 gcttatagcg accatacagc agcaacgacg acatcgcagc aaacgaaagc agggcggcag
      121 gcagcgccac gtaattatat gccagcccca gcgtcagcat catacctccg caatatgcgc
      181 cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat aactctccgc
      241 ctttggcgtt ttgtagtaac aatatttgta gcggtgctga aagggcaaat aatcccgcgc
      301 aacaaataaa agcaaaaata agcgacgttg ttttcatgcc gccgcagaaa aagagcatca
      361 gcagtgccag tacaattata aagtcagtca ctgctgcaat gcgcagtggt gaataacgtc
      421 ctgaaatcct gccacttagc atatttccca gcaccatccc tagcccaact aacatcataa
      481 taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc tttacgtagc
      541 tgaaccaggc aaacacacct gcgttgccaa acatcgtggc ggcgaaaatt aaccacgggg
      601 ccgggctgcg caaaaagtga aattgttcgc gcagatttcc tttcgcctcg tcgcgaatat
      661 ctggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaacagcg atcaataaaa
      721 aggtgtaacg ccagctaaat tcctgactta aatacgttcc cagcggaatg cccagcaaat
      781 tggcgactgt catcccggaa accatccccg ccacggcggc ggtgactttt ccgggtttga
      841 taatttttga taacacgatc gctccgacgc caaaaaatgc gccatgcgga aagccggata
      901 ccagccgacc aatggcgagc atcaggtaag acgaagagag cgtgaacatg gcgttgccaa
      961 tgacgcacaa cgccaccaga aacaacaaga tatgtttgag tgagtagcgg ctggaaaaga
     1021 gtgcgatgat tggcgcaccg accaccaccc ccagtgcata atacgagatc atatgcccgg
     1081 cggcaggaat cgaaattcct acgttatgcg ccagctccgt gagcacgccc ataatgccaa
     1141 attcggccat ccccaaacca aacgtgccca gagccaaaga taaaatgact tttttcatac
     1201 caccatcaca accaccagaa cggctaccaa tcaccgtagc caataaagtg atagcttgca
     1261 ggataacgac cagcaaggcc aagagataat tcgcccctct gtattcatta tcctgctgaa
     1321 tagttatttc actgcaaacg tactttccag tttgctgtag cccaggccgt tgatcttttt
     1381 cactttgatc tgcaccggaa tacgctcttt catcgcttct acgtggctaa tcacaccgat
     1441 ggttttgcca ctggcgttca gggcatccag cgcatcaagg gcggtatcca gcgtttcgct
     1501 atccagcgtg ccaaaacctt catcaaggaa cagcgagtca atacgtgttt tatggctgac
     1561 cagatccgaa agcgccagcg ccagcgccag actaacgagg aaactttcgc cgccggaaag
     1621 ggtacgggta tcgcgtaccg catctgcctg ccaggtatca acaacctcga cttccagcgc
     1681 ctcgctggct ttgcgctgta acagatagcg cccgtgcagc cgggtaagtt gctgattagc
     1741 gagatggact aaattatcca gcgtcagccc ctgggcaaac ttgcggaatt tatcgccctc
     1801 tttggaacct attagcgaat tcagatatcc ccagtcctca acctgctgcg tcatttgagc
     1861 aatttgctgc attaaggttt gttgttgctg acggttatct gcatcctgct tcagctgctg
     1921 gcgaatctcg ccttgactcg tggtgttttc acgcaacttt tggtgagttt gcgctaactc
     1981 ttgctgaatc tgctccaccg tcacagtgag agccaacccg tcaggt
//
LOCUS       DACAPB010000331         2009 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.340, whole genome shotgun sequence.
ACCESSION   DACAPB010000331 DACAPB010000000
VERSION     DACAPB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.340"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..648
                     /gene="agaD"
                     /locus_tag="IT904_004677"
     CDS             <1..648
                     /gene="agaD"
                     /locus_tag="IT904_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAP3194336.1"
                     /translation="DKPGLIAAMKDNLEFINTHPNLVGFLMGLLISMEEKGESRDTIK
                     GLKVALFGPIAGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVG
                     WTHVGYSVGVKAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHS
                     VALQQDFFDKVFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            649..1404
                     /locus_tag="IT904_004678"
     CDS             649..1404
                     /locus_tag="IT904_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HAP3194337.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            1805..>2009
                     /locus_tag="IT904_004679"
     CDS             1805..>2009
                     /locus_tag="IT904_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP3194338.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRNIGERSPK"
BASE COUNT          550 a          435 c          460 g          564 t
ORIGIN      
        1 gacaaaccgg gcttaatcgc ggcgatgaaa gataacctcg aatttattaa tacccacccg
       61 aatctggtcg gattcctgat ggggttatta atttcgatgg aagaaaaagg agaaagccgc
      121 gacaccatta aaggcctcaa agtggcactg tttggcccaa tcgccgggat tggcgatgcg
      181 attttctggt ttactttgtt gccgattatg gcgggaattt gctcatcatt tgccagccag
      241 ggaaacctgc tggggccgat tctatttttc gccgtttacc tgcttatctt tttcctgcgc
      301 gtcggctgga cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa agtgcgagag
      361 aactcgcaga tgattgcccg ttcggcaacc atcctcggga tcacggtaat cggcgggctg
      421 atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag tacccacagc
      481 gtcgcgctgc agcaggattt cttcgataaa gtcttcccga acattttacc gatggcctac
      541 accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct gttaatcggc
      601 gtgacttttg tgctctctat tgtttgttcc gcattcggca ttttgtaaat ggaacgaggc
      661 actgcgtctg gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac ccttcagcaa
      721 gttgaaaact atacggcgtt aagtgaacgt gccagcgaat atttattggc cgtgatccgt
      781 agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact gacgtatcat
      841 tatctggtag aaaaaatcca ccagcagcag gttgatgtca gccagctcac cttcgtgaag
      901 ctcgacgaat gggtggatct gccattaacg atgcctggta cctgcgaaac tttcctgcaa
      961 cagcatatcg tgcagccgct ggggctacgt gaagaccagc tcatcagctt tcgctccgaa
     1021 gagataaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa aggcggtctg
     1081 gatttatgcg ttctcggatt ggggaaaaac ggtcatcttg ggctgaacga accgggagaa
     1141 agcctgcaac cggcctgcca tatcagtcaa cttgatgcca gaacacagca acatgagatg
     1201 ttaaaaaccg cgggtcgccc cgtgactcgt gggatcacct taggcctgaa ggatattctc
     1261 aatgcccgcg aagttttgtt actggtgact ggcgaaggaa agcaggatgc gacagatcgt
     1321 tttctcacgg ctaaagtctc taccgctatc ccggcttcat ttttatggct gcacagtaac
     1381 tttatttgtt taattaacac ctaaccagat gaaaaatttg ctcaaaatgt ttaaattaac
     1441 ttatgtaaca gtcacgcatt atattaaata acatttgact gggttgaaca taacgccgat
     1501 agcaaaagga gttatgttca acaatttaat tttagatata catataactt gttctattaa
     1561 taaatcatct cgtattctga ctcgccccct ggcgcagcaa agtcaggcat ttatactctt
     1621 ttgaatacat agaaaattga tatcaatata atgaaaatat caaatatttg catataaata
     1681 taatcttaaa gttcagtcta tttaatgttc aatgaaatat ttctgcctgt ataatcttta
     1741 aagatgttga acatatattc acattaaata tgattatgta cttgttacaa ggataaggtt
     1801 atatatgaat aaagttacaa aaacagctat tgcgggctta cttgcgcttt tcgcaggtaa
     1861 tgccgctgca accgatggtg aaatagtttt tgatggcgag attttaaaat ccgcttgtga
     1921 aatcaatgac tctgataaga aaattgaagt tgctcttggt cactataatg ccgaacagtt
     1981 tcgtaatatt ggcgagcgta gcccaaaaa
//
LOCUS       DACAPB010000332         1903 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.341, whole genome shotgun sequence.
ACCESSION   DACAPB010000332 DACAPB010000000
VERSION     DACAPB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.341"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            312..>1903
                     /locus_tag="IT904_004680"
     CDS             312..>1903
                     /locus_tag="IT904_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HAP3194339.1"
                     /translation="MTGDLTIKKDTPSVFLRADSGVTALRFYTGDNTERGIIYAGPNT
                     DSLGEVRIRAKTAGGTSGGDLVVRHDGRVEVRDLTVAYKIKSRTIEIANTDTDSSATT
                     LSIYGVQHTPLVLTRSGSSENVSIGFKLDNVNPKYLGIDTNGDLAFGESPDQKQNSKL
                     ITQAKLDKGLTIGGQLAFKGTTAFSAAATFSAGIAGAIEPENIGGQKVDLNNLTIRSD
                     AGAVKYYSCPSSGGGANITNKPDGVTGNFLLRVESTRKVSASDYANMQTLISNDTKRI
                     YVRFVVNGNWTAWSQVVVSGWNQDITVRSLTTSSPVKSGGGRIDVLGSTSDYSKMDCF
                     VRGFDSTGNSLAWALGSSAGVSKMLSLKNFFSGAEILLNGNDGTVQLKTGAVNGATAQ
                     ALTINRNEVNSTVDLTLTKQSGTGNRFVLQNSGNAELPFSVRVWGSSTRQNVFEVGTS
                     AAYLFYAQKTSAGQLFDVNGAINCTTLNQSSDRDLKDDILVISDATKAIRKMNGYTYT
                     LRENGMPYAGVIAQEVMEAIPEA"
BASE COUNT          529 a          409 c          503 g          462 t
ORIGIN      
        1 atgtagagat cttaaaagct ctgcgtgaca aaggtggtca tttctggcgc ggtgataagc
       61 cgaccggaag cacggcgacc atttatagcc acggttctgg tatattctcg cggtgcggcg
      121 atacgtggtc agcgatcaat atcgactact caaccgcgaa gattaagatc tatgccggca
      181 acgatgcccg gcttaacaac gggactttta gcgtcaatga gctatacggc tcggcaaaca
      241 agccgtcgaa atcggatgtt ggacttggca acgtaacgaa cgatgcgcag gtaaaaaaaa
      301 ccggcgatac aatgaccggt gacttgacaa tcaaaaaaga cacgccgtca gtcttcctgc
      361 gggcagacag tggagtcacc gctttgcggt tttatactgg cgataacaca gagcgcggca
      421 taatctatgc tggtcctaac actgattcgc ttggcgaagt tcgcatcagg gcaaagacag
      481 caggggggac atcaggaggg gatcttgttg ttcgtcacga cgggagggtt gaagtccgtg
      541 atctcacagt agcgtataaa attaaaagca gaacgattga gattgcaaat accgacactg
      601 actcatcggc aactacgcta agcatctatg gagtacagca cacgccgttg gttttaacgc
      661 gttctggttc ttctgaaaat gtgtccattg ggtttaagtt agacaacgtg aacccaaagt
      721 atcttggaat tgatactaat ggggatctgg cttttggtga gagtcctgat cagaaacaaa
      781 acagcaaatt gatcacgcaa gcgaaactcg acaagggatt aacgattggt ggtcaactgg
      841 ctttcaaagg tacgacagcg ttttcagccg ctgctacgtt cagtgccggg atagcaggag
      901 ctatcgaacc ggaaaacatt ggcggccaga aggttgatct taacaacctg accatcaggt
      961 cagatgccgg ggcggttaaa tactatagtt gtccatcctc tggaggtggt gcaaatatca
     1021 ccaacaagcc tgacggtgta accggtaact ttttgctccg tgtagagtcg actcgcaaag
     1081 tttctgcttc ggattatgcg aacatgcaaa cgctgatcag caacgacaca aaacgtatat
     1141 acgttcgctt tgttgttaat ggaaactgga cagcgtggag tcaggttgtt gtttccggat
     1201 ggaatcagga tataactgtc aggtcgttaa ccacatctag tccggtaaaa tctggcggag
     1261 ggcggattga tgtccttgga agcacgtcag actatagcaa aatggattgc tttgtacgtg
     1321 ggtttgatag caccggtaat tctctcgcgt gggcgttggg ttcatcagcc ggcgtaagta
     1381 agatgctgtc gctaaaaaat ttctttagcg gagctgagat actgttaaat ggtaatgacg
     1441 gcacggttca actcaaaaca ggtgctgtta acggggctac agcgcaggcg ctcactatca
     1501 acaggaatga ggttaactca actgttgatt taacccttac aaaacaatca gggactggca
     1561 atcgttttgt tttacagaac tcaggtaatg cagaactacc gttttctgtc agggtgtggg
     1621 gttccagtac tcgacaaaac gtttttgagg ttggcacgtc tgctgcgtat ctgttttatg
     1681 cgcaaaaaac gtcagcaggc cagttgtttg atgtaaatgg cgctattaat tgcacaacgc
     1741 tgaatcagtc atcagaccgc gaccttaaag acgatattct cgttatcagc gacgcgacga
     1801 aagcaatccg taaaatgaac ggatacacct acacgctcag ggaaaacggg atgccttatg
     1861 ctggcgttat tgcacaggaa gtaatggagg cgataccaga agc
//
LOCUS       DACAPB010000333         1841 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.342, whole genome shotgun sequence.
ACCESSION   DACAPB010000333 DACAPB010000000
VERSION     DACAPB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.342"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1525
                     /locus_tag="IT904_004681"
     CDS             <1..1525
                     /locus_tag="IT904_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP3194340.1"
                     /translation="DVKVEEEDGRVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGG
                     DSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRS
                     DAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNY
                     QNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVST
                     SLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNEISHTASWYDSSDRNNS
                     WSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATR
                     HGAAFHDYSGSADSRFMIDADGTEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDT
                     RNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIIGVIRLADGSHPPLGISVKDET
                     SHKELGLVADGGFVYLNGIQDDNKLALRWGDKSCFIQPPNSSNLTTGTAILPCISQN"
     gene            1541..>1841
                     /locus_tag="IT904_004682"
     CDS             1541..>1841
                     /locus_tag="IT904_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP3194341.1"
                     /translation="MRYLNTKNIIAAGVLLSCMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMITQEKIA"
BASE COUNT          556 a          400 c          386 g          499 t
ORIGIN      
        1 tgacgtcaag gttgaagaag aggacggacg cgttacgcaa tggcaagttg catctaatag
       61 tattccttat ctgacgcgta aagggcagat tcgctacacc actgctatgg gtaaaccgac
      121 cagcgttggt ggtgattcct tacaacaacc cttcttctgg actggtgaat tctcatgggg
      181 ttggctgaac aatgtatccc tgtatggtgg ttcagtttta acaaaccgtg attatcaatc
      241 tctggctgcc ggcgttggtt ttaaccttaa ctcattaggt tccttatctt ttgatgtcac
      301 acgatctgat gctcagttgc ataatcagga taaagaaacg ggttatagct accgcgctaa
      361 ctattcaaaa cgttttgaat ctaccggtag ccagctcact ttcgctggtt accgtttctc
      421 tgataaaaac tttgtgacaa tgaatgaata tatcaatgac actaaccatt acacgaatta
      481 tcagaatgaa aaagagagtt atattgtcac gtttaaccag tatcttgaat cattaaggtt
      541 aaatacatac gtaagtttgg ctcgtaatac ttactgggac gccagcagta atgtgaatta
      601 ttcattatca cttagccgcg attttgatat cgggccatta aaaaacgtct ccacttctct
      661 aacatttagc cgaataaact gggaagaaga caaccaggat caactgtacc taaatatttc
      721 gattccctgg ggaactagta gaacattgag ctatggtatg caacgaaatc aggataatga
      781 gatttcgcat actgcttcgt ggtatgactc ttccgatcga aataattcct ggagcgtttc
      841 tgcttcaggc gacaatgatg aattcaaaga tatgaaagcg tcactacgcg ccagttatca
      901 gcataatacc gagaacggtc gactctacct ctccggtaca tcacagcgag acagttatta
      961 ttctctgaat gccagttgga atggttcatt cactgcgact cgccacggtg ccgctttcca
     1021 cgactatagc ggtagtgctg actcgcgttt tatgatcgac gcagacggca ctgaagatat
     1081 tccgttgaac aataaacgcg cggtaactaa tcggtatggc atcggagtta ttccatcagt
     1141 cagcagttac ataacaacat cattaagtgt tgatacccga aatctgccag aaaatgtgga
     1201 tatcgaaaac tcggttatca ccaccacctt aaccgagggt gctattggct acgccaaact
     1261 tgatacccgc aagggctacc aaatcatagg ggttattcgc ctggcagatg gtagtcatcc
     1321 accactgggg attagcgtaa aagatgaaac cagccacaaa gaattaggac tggttgctga
     1381 tggcggcttt gtatacctca acggcattca ggatgataac aaacttgctt tacgctgggg
     1441 tgacaaatct tgttttattc aaccacccaa tagcagcaac ttaaccaccg gaacggctat
     1501 tttaccgtgt attagccaaa attaattcag gatgaaacta atgcgctact taaatacaaa
     1561 gaatatcatt gcagcaggag tattactctc ctgtatgagc agcattgcct ggggtgccat
     1621 catcccggat cgtacccgta tcataatgaa tgagtcagat aaaggcgaag cattaaaact
     1681 tactaatcaa agtaaaaacc tgccctatct ggcccaaaca tggatagaag atacgaaagg
     1741 caataaatcg cgtgacttta ttgtcactgt tcctccaatg gtgcgtttaa atcccagtga
     1801 gcaaattcaa ataagaatga taactcagga aaaaattgct c
//
LOCUS       DACAPB010000334         1817 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.343, whole genome shotgun sequence.
ACCESSION   DACAPB010000334 DACAPB010000000
VERSION     DACAPB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.343"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(328..1563)
                     /locus_tag="IT904_004683"
     CDS             complement(328..1563)
                     /locus_tag="IT904_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAP3194342.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAEHHFLVGLSLDGPPEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPEEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1710..1786)
                     /locus_tag="IT904_004684"
     tRNA            complement(1710..1786)
                     /locus_tag="IT904_004684"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1750..1752),aa:Pro,seq:tgg)
BASE COUNT          455 a          505 c          429 g          428 t
ORIGIN      
        1 tcatcctgtc agggagaggg atctcaatta tcaatgctta attacgtcat cattttgatg
       61 acatgaaacc ggagcatttt gcatggaatt ttcgttttca cccaaacttc ttgttgttgc
      121 tgtcgccgcc gctcttcctc tcatggccag cgcagcagat accccgtcaa ctgccaccgc
      181 acgcaaaggc tttgccggat acgatcaccc aaaccagtat ccacagaacg ctgcaattta
      241 ttgaatttgc acgatcatgt aggccggata aggcgtttac gccgcatccg gcaatcaacc
      301 gcaggcggcc acaaaatggc cgctttttta cttactcacc accagcaaat gcgcatgcat
      361 aatgtcactg gccgggcgac cgtgcgccag caaatcagcc attgctttaa gatatggcgg
      421 tagatggcgg aaataacgct gatacccggc acacaaataa ttcagccccg gtttgccgct
      481 ggcatcgagc atgaagcggt gtttcgggca gcctccccag cacgctttta acacgttaca
      541 actgcgacac tgcgccggta attgcttaaa tttatcttca ccaaacacct gctgttgcgg
      601 ggaatcgatc atttctgcaa ttgtttgctg gtgcatattc cccagccgat attgtggata
      661 aacatagtga tcgcaggcgt aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca
      721 ggttggctga tgatggcaaa ccgcacccgg cgcaccgaca aaattggcaa acgcccattc
      781 gatattcatc acgaaaatct tgccgacgtc gcgtttgatc cagtggtcga atatcgccac
      841 cagaaattca ccgaactcct cggggcgcac cgaccattcc gttagctcac cctgaatatc
      901 accaggcgca tgcaatttca gtccttcgcg ggcagccgtt tcatcagcca ggcgctcgac
      961 caccggaata aactggatga attccactcc cgcatcgcac aaaaaatcat atacctgcaa
     1021 cggttgctgc gcgctggtgc gattaacgca gaccagcacg ttatagtcga catgatgttt
     1081 ttgcagcagc gtcagggcac gcatcaccag cttatgcgtg ggtctgccac ctttagtcac
     1141 gcgatattga ttgtggatct caggcggacc atccagcgat aaaccaacaa gaaaatgatg
     1201 ctccgcgaga aacgcgcacc attcgtcatc cagcagcacg ccgttagtct ggaagctgtt
     1261 acttatcttc ctgccagcac catatttcgc ctgtagcgct acggcacggc ggtaaaactc
     1321 cagcccgagt agcgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgcagttc
     1381 gctggcggcg atatagtgac ggatatacgc ttccagcgtg tcatcgtcca tatgcgtgac
     1441 tggcttttcg cggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt
     1501 acaatcggaa ccactcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgcag
     1561 catggtcact ccttgcctga agtcatcctt caatacgtta agggcgaaat cataccgtgg
     1621 ggcaatgagt tattacgggc gtttattgcg aagggagggt gacgaaatgc acagaaaaca
     1681 aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg aacctccgac
     1741 ccactggtcc caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt
     1801 gaatttttag ttcaatt
//
LOCUS       DACAPB010000335         1816 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.344, whole genome shotgun sequence.
ACCESSION   DACAPB010000335 DACAPB010000000
VERSION     DACAPB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.344"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..373
                     /gene="ompC"
                     /locus_tag="IT904_004685"
     CDS             <1..373
                     /gene="ompC"
                     /locus_tag="IT904_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAP3194343.1"
                     /translation="DANNIYLAAQYTQTYNATRVGSLGWANKAQNFEAVAQYQFDFGL
                     RPSVAYLQSKGKNLGVVAGRNYDDEDILKYVDVGATYYFNKNMSTYVDYKINLLDDNQ
                     FTRDAGINTDNIVALGLVYQF"
     gene            485..1540
                     /gene="apbE"
                     /locus_tag="IT904_004686"
     CDS             485..1540
                     /gene="apbE"
                     /locus_tag="IT904_004686"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAP3194344.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            1614..>1816
                     /locus_tag="IT904_004687"
     CDS             1614..>1816
                     /locus_tag="IT904_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="regulates genes involved in the repair of alkylated
                     DNA; repairs DNA containing 6-O-methylguanine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme protein Ada"
                     /protein_id="HAP3194345.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRP"
BASE COUNT          453 a          478 c          472 g          413 t
ORIGIN      
        1 cgacgctaac aacatctacc tggctgctca gtacacccag acctacaacg caactcgcgt
       61 aggttccctg ggttgggcga acaaagcaca gaacttcgaa gctgttgctc agtaccagtt
      121 cgacttcggt ctgcgtccgt ctgtagcata cctgcagtct aaaggtaaaa acctgggtgt
      181 cgttgctggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac
      241 ctactacttc aacaaaaaca tgtccaccta cgttgactac aaaatcaacc tgctggacga
      301 caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt
      361 ttaccagttc taatctcgat ggatatcgaa caaagggcct gcgggccctt ttttcattgt
      421 tttcagcgta caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc
      481 acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct tttttgttgg
      541 ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg
      601 caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgatgcca aacgcagtgc
      661 cgaacttaaa gaaaagattc agacccagct ggacgccgac gatcagctgc tttcgaccta
      721 taaaaaagat tccgcgctga tgcgctttaa cgattcgcaa agtttatcgc cgtggccggt
      781 aagtgaagcg atggccgata tcgtcaccac ctcgctgcgc attggcgcga agaccgatgg
      841 cgcgatggat ataaccgtcg ggccgctggt gaatctgtgg ggctttggcc cggaacaaca
      901 gccggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca
      961 gcacctgacg gtcatcaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt
     1021 atatgtcgat ctctccaccg tcggtgaggg ttatgcggcg gatcacctgg cacgcttgat
     1081 ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg
     1141 tatgaacggt gaaggccagc cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa
     1201 cgcggttcaa gctgtggtgg atatcaacgg tcatgggatt agcacctctg gcagctaccg
     1261 taactattac gaactggacg gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg
     1321 tcccatcgaa cacaatctgg tatccgtgac ggtgattgct ccgacggcgc tggaagccga
     1381 tgcctgggat actggcttaa tggtactcgg gccggagaaa gccaaagagg ttgttcgccg
     1441 ggaagggctg gcggtttata tgatcaccaa agaaggcgat agctttaaaa cctggatgtc
     1501 accacagttt aaaagcttcc ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt
     1561 tgcgtgatgg tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaaa
     1621 acgccacatg cttaactgac gatcaacgct ggcaatctgt cttagcccgc gacccgaatg
     1681 ccgacggcga attcgttttc gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc
     1741 gcgccagaca tgccttgcgg gaaaatgtct ccttctacgc aaatgccagc gaggcactcg
     1801 ccgccggatt tcgccc
//
LOCUS       DACAPB010000336         1770 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.346, whole genome shotgun sequence.
ACCESSION   DACAPB010000336 DACAPB010000000
VERSION     DACAPB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.346"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>1770
                     /gene="rpoB"
                     /locus_tag="IT904_004688"
     CDS             <1..>1770
                     /gene="rpoB"
                     /locus_tag="IT904_004688"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP3194346.1"
                     /translation="VIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQ
                     QVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTA
                     VAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLG
                     EPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIH
                     IQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPK
                     GETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEI
                     EEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEE
                     KQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGD
                     KMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAK
                     GIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGM
                     PIATPVFDGAKEAEIKELLKLGDL"
BASE COUNT          451 a          444 c          505 g          370 t
ORIGIN      
        1 cgttatcgcc caggcgaact ccaacctgga tgaagaaggc cacttcgtag aagacctggt
       61 aacttgccgt agcaaaggcg aatccagctt gttcagccgt gaccaggttg actacatgga
      121 cgtatccacc cagcaggtgg tatccgtcgg tgcgtccctg atcccgttcc tggaacacga
      181 tgacgccaac cgtgcattga tgggtgcgaa catgcaacgt caggccgttc cgactctgcg
      241 tgctgataag ccgctggttg gtactggtat ggaacgtgct gttgccgttg actccggtgt
      301 aactgcggta gctaaacgtg gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat
      361 caaagttaac gaagacgaga tgtatccggg tgaagcaggt atcgacatct acaacctgac
      421 caaatacacc cgttctaacc agaacacctg tatcaaccag atgccgtgtg tgtctctggg
      481 tgaaccggtt gaacgtggcg acgtgctggc agacggtccg tccaccgacc tcggtgaact
      541 ggcgcttggt cagaacatgc gcgtagcgtt catgccgtgg aatggttaca acttcgaaga
      601 ctccatcctc gtatccgagc gtgttgttca ggaagaccgt ttcaccacca tccacattca
      661 ggaactggcg tgtgtgtccc gtgacaccaa gctggggcca gaagagatca ccgctgacat
      721 cccgaacgtg ggtgaagctg cgctctccaa actggatgaa tccggtatcg tttatattgg
      781 tgcggaagtg accggtggcg acattctggt tggtaaggtt acgccgaaag gtgaaactca
      841 gctgacccca gaagaaaaac tgctgcgtgc gatcttcggt gagaaagcct ctgacgttaa
      901 agactcttct ctgcgcgtac caaacggtgt atccggtacg gttatcgacg ttcaggtctt
      961 tactcgcgat ggcgtagaaa aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa
     1021 acaggcgaag aaagacctgt ctgaagaact gcagatcctc gaagcgggtc tgttcagccg
     1081 tatccgtgct gtgctggtag ccggtggcgt tgaagctgag aagctcgaca aactgccgcg
     1141 cgatcgctgg ctggagctgg gcctgaccga cgaagagaaa caaaatcagc tggaacagct
     1201 ggctgagcag tatgacgaac tgaaacacga gttcgagaag aaactcgaag cgaaacgccg
     1261 caaaatcacc cagggcgacg atctggcacc gggcgtgctg aagattgtta aggtatatct
     1321 ggcggttaaa cgccgtatcc agcctggtga caagatggca ggtcgtcacg gtaacaaggg
     1381 tgtaatttct aagatcaacc cgatcgaaga tatgccttac gatgaaaacg gtacgccggt
     1441 agacatcgta ctgaacccgc tgggcgtacc gtctcgtatg aacatcggtc agatcctcga
     1501 aacccacctg ggtatggctg cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca
     1561 gcagcaagaa gtcgcgaaac tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga
     1621 cgttcgtcag aaagttgacc tgagtacctt cagcgatgaa gaagttatgc gtctggctga
     1681 aaacctgcgc aaaggtatgc caatcgcaac gccggtgttc gacggtgcga aagaagcaga
     1741 aattaaagag ctgctgaaac ttggcgacct
//
LOCUS       DACAPB010000337         1717 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.347, whole genome shotgun sequence.
ACCESSION   DACAPB010000337 DACAPB010000000
VERSION     DACAPB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.347"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(72..164)
                     /locus_tag="IT904_004689"
     tRNA            complement(72..164)
                     /locus_tag="IT904_004689"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(128..130),aa:Ser,seq:gct)
     gene            complement(480..665)
                     /gene="csrA"
                     /locus_tag="IT904_004690"
     CDS             complement(480..665)
                     /gene="csrA"
                     /locus_tag="IT904_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAP3194347.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(900..>1717)
                     /locus_tag="IT904_004691"
     CDS             complement(900..>1717)
                     /locus_tag="IT904_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP3194348.1"
                     /translation="PEEIRAVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKY
                     DERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATV
                     HADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKA
                     IDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGSTIIVLATVVEGKVSLIAGVSKDVT
                     DRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ"
BASE COUNT          435 a          469 c          376 g          437 t
ORIGIN      
        1 cgggaggatt cgaacctccg accgctcggt tcgtagccga gtactctatc cagctgagct
       61 acggatgcaa atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata
      121 ctcccttagc aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag
      181 tgcttcgaag aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga
      241 gacttataag tcaaacaatt tttcctacac ttttatcgtt tgcacacttc acgttcaatt
      301 agtctgtaaa aacgtcaaaa agagtgtttt atcaacagaa gaatggaggt ctgacagata
      361 gtagtaatgc aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta
      421 tagagtgaag gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat
      481 tagtaactgg actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga
      541 acagaaactt ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa
      601 actgtcacgg tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc
      661 agcattcttt gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc
      721 catcatataa cgccaaaaag taagcaatga caaacacatt acatctaagc agtcatggca
      781 ttacattctg ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt
      841 gtcgatagcg ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat
      901 tattgcaatt tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca
      961 gcatccgtac caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc
     1021 tgagcgacca taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg
     1081 cctgcaatca gagaaacctt accttcgact accgttgcca gcacgataat tgtcgacccc
     1141 agctgatttt ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc
     1201 tcgctaacca acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc
     1261 tcctgtgcgg cagcttgttc tttaagctgt tgtaactctt tttccagctg acgcgtacgt
     1321 tccagtactg agcgcacttt atcagccaga ttattgctat cgcctttcag cagatgcgcg
     1381 acttcgctta agcgatcgct gtctgcatga acggtggcga tagcaccttc tccggttacc
     1441 gcttcgatac gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca
     1501 atatcaccag tgcggctggc gtgagtaccg ccacacaact cggtagagaa atcgcccatg
     1561 ctcagcacgc gtacgcgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct
     1621 ttcgccgctt cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgtg
     1681 ttcaccaggt cttcgaccgc acgaatctct tctggtt
//
LOCUS       DACAPB010000338         1710 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.348, whole genome shotgun sequence.
ACCESSION   DACAPB010000338 DACAPB010000000
VERSION     DACAPB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.348"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            77..637
                     /locus_tag="IT904_004692"
     CDS             77..637
                     /locus_tag="IT904_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAP3194349.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            644..877
                     /gene="mbeD"
                     /locus_tag="IT904_004693"
     CDS             644..877
                     /gene="mbeD"
                     /locus_tag="IT904_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAP3194350.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
     gene            complement(1361..>1710)
                     /gene="exc2"
                     /locus_tag="IT904_004694"
     CDS             complement(1361..>1710)
                     /gene="exc2"
                     /locus_tag="IT904_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HAP3194351.1"
                     /translation="KTSLERHTRHYVYASDDGFDPNFYTQKADTIRMMLPFFQQFRDM
                     GMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTAFAGRKYTNSDMPSPKKMKLMADT
                     ISAVYLDGYEGRQ"
BASE COUNT          436 a          367 c          524 g          383 t
ORIGIN      
        1 gtgaggacaa ccaggagcag tatcagtatc gccgacccgg agggtgggcg taatctcagg
       61 ctgaaagggg cgctgtatga gcaatctttt acaaatgggg acagacttcg agaagaaact
      121 gaaagagcgt gccgtctcta cagagaacat gctgaacagc gagttcagga agctcgaagc
      181 gtctgtagac agggaactga acttaaacgg gcagaaaatc agagacgcta tcagcgcaca
      241 cacagcagcg gtgaaagaac aactggagac gctgaacagt gccgtgaacg cgcagttcag
      301 cgccacagag aacgaactgg cccggcggca ggaagagctg ctctggaagc tggtcaaagg
      361 gcggatactg tacccgtcgc tgacggcgct ttgcgtcacg ggcgggattt ttctggcgtc
      421 atgggggctg attcagtggc aggaaagccg gattgcggcg aacatcctgg ctatcaggga
      481 ccaggaagag acgctggcga aactccggga aaaaacatgg ggtgtgacgt atcacgaggg
      541 caggaacggg aaatttctgg tgttgccgtc cggggtgaag ggggaaaaca actggacggt
      601 ggagaagaag aacgcagtaa gactggtcag ggagtaagtg agtatgacag agctggaaat
      661 gcacttactg aacgccttag agcaattgca gcaggactat acgcagcggc tgaaagactg
      721 ggagagcgcc ttcacggatt tgcagcatat gttcgagctg acacgacagg agaacgggca
      781 actgagggaa cagtgcgacg acttgagtca gcaggtgcag agattagccg ggcaggtgga
      841 ccacttgagt cggttattta tcacgaacag cagataaggg atgaacagga aaggcaggca
      901 cgcctggaac gggagcgtgc gcggtcctgg catgaagagc aggaacggga agcacgggta
      961 tggcgtggcc cgtcaatagg gatgtgacgg tgagttgttc cgggaggttg ccagatggta
     1021 ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg tgtcgttttt gttgctgtgt
     1081 tttgcctctt tgccagatcg cgctcattcc tcagcctcat cccgcgatgt ctgcgtatgt
     1141 catcaactga aaagatggaa aaaaaacagt gtcatgaggt ttacgcaggc aaaactagcg
     1201 ttattcacat ggctgaatag ctatgcatac ggttaaaatt tatcaggtgc gatcgcggca
     1261 gcttttcggg tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc gctgaacgca
     1321 tttcagcggt gcgtacaatt aagggattat ggtaaatccc ttactgtctg ccctcgtatc
     1381 catcgagata aaccgcagaa atggtgtctg ccattagttt catttttttc ggcgaaggca
     1441 tatcgctgtt tgtgtatttt ctgccagcaa aggctgttgt gctccggagt gagtgaaaaa
     1501 atttttctga gtggaattct tttacacgtt gctgtgccgt ttctgctgat actccggctg
     1561 ctttgtcttt catccccata tcccggaact gctgaaagaa cgggagcatc atacgtatgg
     1621 tgtctgcttt ctgggtgtag aagttaggat caaatccatc atctgaagca taaacataat
     1681 gccgggtatg tcgttccagg ctggtctttg
//
LOCUS       DACAPB010000339         1703 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.349, whole genome shotgun sequence.
ACCESSION   DACAPB010000339 DACAPB010000000
VERSION     DACAPB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.349"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..199
                     /locus_tag="IT904_004695"
     CDS             <1..199
                     /locus_tag="IT904_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194352.1"
                     /translation="IHEKNKNMDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPV
                     TVTLDIITAPLQFLVVYAVNQ"
     gene            complement(245..1057)
                     /gene="yidA"
                     /locus_tag="IT904_004696"
     CDS             complement(245..1057)
                     /gene="yidA"
                     /locus_tag="IT904_004696"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP3194353.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(1172..1579)
                     /locus_tag="IT904_004697"
     CDS             complement(1172..1579)
                     /locus_tag="IT904_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAP3194354.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
BASE COUNT          421 a          433 c          409 g          440 t
ORIGIN      
        1 aatccatgag aaaaataaaa acatggatta ctcaaaggtg atggcgttct accatccatt
       61 taaagtgcga ttttatgaat actattcacc cagaggcatt ccggatggtg tttccgcagc
      121 attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat ttctggttgt
      181 atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt tgcccgccca
      241 cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca aggttagatt
      301 tggtgacaaa gttcgccact tctttcactg aaggaatagc gttatccatc gccacaccga
      361 cgcctgcata ttcaatcatt gcgatatcgt tttcctgatc gccaatcgcc atgatttctt
      421 ccggtttaat acctaacacg tcggccagtg atttcacccc cgtacctttg ttaacgcgtt
      481 tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc tctttcactt
      541 cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc atcatcactt
      601 tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc ggaattgtgg
      661 caacgaagga ttcatgcacc gtgtagtagc tgatatcacg gttggcggtg tacagcgtgg
      721 tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc aggaaacggt
      781 agtcgtcata gctgagagcg gtttgcgcca cggtgctacc atcagcggcc ttctgtacca
      841 gcgcgccgtt ataagtaatg cagtagtcgc ccggcagttc catatgcagc tctttcaggt
      901 aattgtgaac acctgcataa gggcgacccg tcgttagcac gacattcacg ccacgggcgc
      961 gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggt agcagaaggg
     1021 tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt agattagttc
     1081 ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg gaaaaggggg
     1141 atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct ttcagcagtt
     1201 ccatgcctgc ggaaagcaga tcgttattgg cttgtggaga cacttcacct tgcggtgaga
     1261 gcgcatcaat aatcttcggc aattgttctg ccagtaaact ggaagctgta ctggtatcca
     1321 cgccaagttt ttgtccaaga tcggacaccg catttgtgcc gagcgccgat tccagttgct
     1381 cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga atggccccta
     1441 agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc tcaacccaac
     1501 ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa
     1561 aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt atagatttgc
     1621 gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg cccttttctt
     1681 atagcttctt gccggatgcg gcg
//
LOCUS       DACAPB010000340         1692 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.350, whole genome shotgun sequence.
ACCESSION   DACAPB010000340 DACAPB010000000
VERSION     DACAPB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.350"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..285)
                     /locus_tag="IT904_004698"
     CDS             complement(<1..285)
                     /locus_tag="IT904_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194355.1"
                     /translation="MRKLWNALRRPSAHWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLE"
     gene            415..1107
                     /gene="torR"
                     /locus_tag="IT904_004699"
     CDS             415..1107
                     /gene="torR"
                     /locus_tag="IT904_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAP3194356.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QSVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(1080..>1692)
                     /gene="torT"
                     /locus_tag="IT904_004700"
     CDS             complement(1080..>1692)
                     /gene="torT"
                     /locus_tag="IT904_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAP3194357.1"
                     /translation="KSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMPGPDNAGGSKE
                     MVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAGTAIAAEAAMG
                     EGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMVWQGELAVEQAIRQLQGQSVS
                     DNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
BASE COUNT          405 a          459 c          431 g          397 t
ORIGIN      
        1 ttccagtttg cgcttcacca tgcctggaat atccggcggg atatgacagt catggcattc
       61 agctcgcacg ccggaggcgt tctggaaatg caccgactgt ttatattctt catacaccgg
      121 ttgcatactg tggcaactga cacaaaattc ggttgtgctg gtgactttga tcccaacgtg
      181 tggcaataca atcagcgcaa tgccaatcac aatcccaatt gcgaccagcg ccagtaccga
      241 ccaatgagca ctgggtcggc gtagcgcgtt ccagagtttc cgcataataa cccctgtaga
      301 attatggttt agtgaagcga tcttaatgag caaatatgaa cagcggcact ggtcaggatg
      361 aacggcttac ggcagaatat gaacagatat gaacagaatg agtaaacccc tctgatgcca
      421 catcacattg ttattgttga agatgagccg gttacccagg cgcgattaca atcctacttc
      481 actcaggagg ggtataccgt ttccgttacg gcgagcggtg ccgggctacg ggaaattatg
      541 cagaatcagt cggtagattt aattctgctg gatatcaact tacccgatga aaatggcctg
      601 atgttaaccc gcgccctgcg agaacgctca acggtgggga ttattctggt taccggacgc
      661 agcgatcgga ttgaccgtat tgttgggctg gaaatgggcg cagacgatta cgtcaccaaa
      721 ccgctggaac tgcgcgaact ggtagtacgg gtgaaaaatc tgctctggcg aatcgacctc
      781 gcgcgacaag ctcaaccgca cactcaggac aactgctatc gctttgccgg ttattgcctg
      841 aatgtgtcgc gccatacgct ggagcgggat ggcgagccga ttaaactgac ccgcgcagag
      901 tatgaaatgt tggtggcatt tgtgacgaat ccgggcgaaa ttctcagccg tgaacgtctg
      961 ctacgtatgc tttctgcgcg tcgggtggaa aaccctgacc tgcgcaccgt cgatgtgtta
     1021 attcgtcgtt tacgtcataa actcagcgcg gatttactgg tgacgcaaca tggtgaaggt
     1081 tatttcttag ccgctgatgt gtgctgataa aaatagaccg gacgaaatcc ccctggtgac
     1141 agcgagcggc gaatatgttc acggtcggca tttttcggcg tcagaactaa aatcggtggg
     1201 ctgacattat cagacaccga ttgcccctgt aattgcctga tggcctgctc aaccgccagt
     1261 tccccctgcc agaccatttg atcgctggca gccataatca ctcttccccg cttcagcccg
     1321 cgatacacct gatgtgaaag ataaaacgac accacggtaa gcggcgtttt caggttacgc
     1381 ccttccccca ttgccgcctc tgccgcaatg gccgttccgg caacgacgtc gatttctgga
     1441 tggcgttcca gcatctcctg caacaggtta cgctggattt caatatcgtt atcaccaagc
     1501 gcaatatcaa caatacgcac cgggcttccg gcaatggctg cgcgaaaacc ctcgaccatc
     1561 tccttactgc ccccggcgtt atcgggtccg ggcatcaaca gcacattcag tggtttaccg
     1621 tgcgcccatt gcaccagata tcgccccggt tgatagccca tctgaaacca gggcacacca
     1681 acgcggcttt tc
//
LOCUS       DACAPB010000341         1678 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.351, whole genome shotgun sequence.
ACCESSION   DACAPB010000341 DACAPB010000000
VERSION     DACAPB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.351"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..758)
                     /gene="melB"
                     /locus_tag="IT904_004701"
     CDS             complement(<1..758)
                     /gene="melB"
                     /locus_tag="IT904_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAP3194358.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREPLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITG"
     gene            complement(861..>1678)
                     /gene="melA"
                     /locus_tag="IT904_004702"
     CDS             complement(861..>1678)
                     /gene="melA"
                     /locus_tag="IT904_004702"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP3194359.1"
                     /translation="EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYP
                     ELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGRE
                     DLIERYKVPLDEYPKRCVEQLANWHKELEEYKKASRIDIKPSREYASTIMNAIWTGEP
                     SVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTL
                     LTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR"
BASE COUNT          452 a          431 c          403 g          392 t
ORIGIN      
        1 aagccggtaa taatgttgct ggctacatta taagcaagag ccatacccaa gaggcatgaa
       61 agctgatcgt ttttataaat tagcgcaacg atggctttaa gtgtcagatg gcttccttca
      121 gcagacggtt gattgtctga cgaaaagact tcatgcacat tgcgcagagt gatgatggtt
      181 gaaacaataa aaaaggcgat cagtaccaga gtgaacatct gaaagccaaa tccccgatcg
      241 ccaccgccga cataattaac aaatggtagc gtcacacctg ccgtaacaaa gcctgccaga
      301 ctggcaaaaa aacgcggata aggaaccagt ggttcgcgct cacgtttatc gagcgtgatg
      361 gttggaacca gcgaccagaa gggaatatcc ataatggtgt aagtcatgcc ccagaggatg
      421 taggtcacgc aaacaaagac aatctgagta gtaccttcaa acagatgcgc actaaagagg
      481 agaaataaga ttacagagtt tgccaacgta ccgatcagga tccagggttt gaacttaccc
      541 catcgcgatc gcgtagcatt tacaatccat cccataatcg gatcgttaat agcatcccag
      601 atcctcgcca ccagaaacaa agtaccgacc aaacccacag acagcccgac gacatcggtg
      661 tagtaataca tgaggtacat atacacaatg ccgatcgcaa aatccttccc gaacgctcca
      721 aatccataac tgagttttgt agtcattgaa atgctcatag ggtatcgggt cgctgttacc
      781 aacgcccgcc ttctgttatt ggtatcgtca gaggtgtgcg ccggaataaa tcccccggcg
      841 cagtagcgtt tagtcgcgtt ttaacggtgc aaccagcctg gcagccagtc gccgtgggcg
      901 gcaatcaggt cgtcaacaag agcatatatt tcgtcaatgc ccagcacggc ggcagtatgc
      961 gggtccatca tcgcggcgtg gtaaacacgg tcgcgatttt ccgtaagaat agcttcggtc
     1021 agcagcgtct gtacgttgat gttggtttgc atcagggcgg ccagatgcga aggtagcgta
     1081 ccgactttgg tcggctgaat gccattagca tcaaccagac aggctacttc cacgcaacat
     1141 ccttgtggca ggttatcaat caaaccatcg ttacggacgt tgccgtaaat cacactcggc
     1201 tcgccagtcc agatagcgtt catgattgtg ctggcatatt cccgtgacgg tttaatatca
     1261 atccgggagg cttttttata ctcctccagc tctttatgcc agttcgccag ctgctcgacg
     1321 cagcgtttcg ggtactcatc cagcggtact ttataacgct caatcaaatc ctcacgacct
     1381 ggcttaataa accacggtgt gtactcagca aaatgttctg acgattccgt gacgaaatag
     1441 cccagctttt tgaacatttc gtagcgcaca atattctggc agcgagtatt gccatgaata
     1501 ttcggcttcg gtgcctgccc tgcttcataa gccgccagca gttccgggta gagattcaca
     1561 taactgccgt cggcggtttt gcgctccagc tccaggtaaa acgccatatg gttgatacct
     1621 gcgcaacggt aacgcagcgt agctgggtcg atattgaggt cacgcgccaa ctcttccg
//
LOCUS       DACAPB010000342         1667 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.352, whole genome shotgun sequence.
ACCESSION   DACAPB010000342 DACAPB010000000
VERSION     DACAPB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.352"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            131..607
                     /locus_tag="IT904_004703"
     CDS             131..607
                     /locus_tag="IT904_004703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194360.1"
                     /translation="MNDSKEAKIATLSNIQSIINRLADTSSKLKATYITIISAILTVF
                     MTYIIPKKEGIEGPFNPEDKLYSIIIFLVIFLAFLLIDSYYLRYERVMRKVYDSKARD
                     IPDDINSAINYFDISATYKKILKEGGVSYMPSKSQLLFYFIPFLVISITISMMVLR"
     gene            612..1448
                     /locus_tag="IT904_004704"
     CDS             612..1448
                     /locus_tag="IT904_004704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194361.1"
                     /translation="MQKKYTIKLEQLRTDLDDYKKSRFVHSDKLMYDGINVNDRDRLI
                     TEIKNYIKDNDVINTETIINKLFPTKTPHLFISHKSEDAKEAITLANILHSKFGIESF
                     IDSQVWHHINDIQKILDEEFSKKSENLYDYKKSNIVSSNIFSMLSSSLYRTIDDSDGF
                     IYIDRNDAHEDIGISKSNMVNIKTESPWIYLENIYTNSLRIKKHNRERIILEKSGLNN
                     YAADSQVSIEERKEPKFIYSTNLVNAVKIKSLNNAFNVQYDKTKKHPLEGLDYFYNII
                     LV"
BASE COUNT          672 a          294 c          230 g          471 t
ORIGIN      
        1 tcctcaactt taacactgct cggtccaatc aaacaccaca ttgactcata ctgtattaat
       61 tacttctcgt atttttttat tacctatgga ataatttaga gataaactta tagcaaaaaa
      121 ggagagtctt atgaacgata gtaaagaagc caaaatcgca acactaagca atatccaatc
      181 aataattaac agactagcag atacctcttc aaagctaaaa gcaacataca taacaatcat
      241 ttcagcaatt cttactgttt ttatgacata tataataccc aaaaaagaag gtatagaagg
      301 cccattcaac ccagaagaca aattatatag cataataata tttctggtta tttttttagc
      361 atttcttcta atagactctt actatcttag atatgaaagg gttatgcgaa aagtttatga
      421 ctcaaaggcg cgtgacattc cagatgatat aaacagcgct ataaattact ttgatattag
      481 cgcgacgtat aaaaaaatcc taaaagaagg tggcgttagt tacatgccaa gcaagtcaca
      541 gcttttattt tattttattc cctttcttgt tatatccata acaatatcca tgatggtact
      601 gaggtgatga aatgcaaaaa aaatacacta taaaacttga acagttgcgt actgatttag
      661 atgattacaa aaaaagcaga tttgttcatt cggacaaatt aatgtatgat ggtataaacg
      721 tcaacgatcg agacaggtta ataaccgaaa taaaaaatta catcaaggat aatgatgtaa
      781 taaacacaga aaccatcatt aataaactct tcccgacgaa aaccccgcac cttttcatat
      841 cacacaaaag tgaggatgcg aaagaagcaa taacacttgc aaacatatta cactcaaaat
      901 ttggcataga atcatttatt gactctcaag tatggcatca cataaacgac attcaaaaaa
      961 tacttgatga agagttttca aaaaaaagcg aaaacttata tgattacaaa aaaagcaata
     1021 tagtatccag caatattttc tcaatgcttt catcctcgct ttacagaacg atagatgact
     1081 cagatggatt tatttatata gatagaaatg acgcacacga agacattggc atatcgaaat
     1141 caaacatggt taatattaaa acagaatcac catggattta tttggaaaac atatacacaa
     1201 actcattaag aataaaaaaa cacaatcgag aaagaatcat tttagaaaaa tctgggctga
     1261 acaactacgc agcagattca caagtatcta tagaagaaag gaaagaacca aaatttattt
     1321 actcaacaaa cctagtaaat gcagtgaaaa tcaaaagcct caataacgca tttaacgttc
     1381 aatatgacaa gacaaaaaag caccccttag aaggccttga ttatttttac aatatcatct
     1441 tagtatagta ttaacgcata gggttatatt ttattgccct atgcgttttc atctgtaaaa
     1501 taataactcc gctttaataa agcagagaaa gtcatcactc caaatcaaaa atccttatgg
     1561 atatctataa cttcactttc taactgagat tcctgaatct gaggattatc taaaagtcat
     1621 aatcacaaat aataaaaatt acagaacaaa tattcagaag tcagcct
//
LOCUS       DACAPB010000343         1603 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.353, whole genome shotgun sequence.
ACCESSION   DACAPB010000343 DACAPB010000000
VERSION     DACAPB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.353"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            19..342
                     /locus_tag="IT904_004705"
     CDS             19..342
                     /locus_tag="IT904_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP3194362.1"
                     /translation="MEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSIIKENLS
                     LHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHCRKAFSD
                     AKVFK"
     gene            339..1325
                     /locus_tag="IT904_004706"
     CDS             339..1325
                     /locus_tag="IT904_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194363.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFIFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          376 a          310 c          384 g          533 t
ORIGIN      
        1 gcctggcaca cgcgtttgat ggaagacggc gttcaggcaa tgcgcgatta tcttgccgga
       61 ctggatatcg cttcgccaga gcatcaggtt ctgatgaacg taacagcaaa aagcgaggtc
      121 gctccgtcaa ttatcaaaga aaatctctca ttacacttaa ctcatacggt gaaatggact
      181 gaatctctcg atacatttct gaatatgcca accccggtag ctttccttga aataagtaat
      241 aagccctatt tggggaatat gttaaatgat tttgctggcg tggatcagca acgagttatg
      301 cattgtcgta aagcatttag tgatgcaaag gtattcaaat gattcctgat tatttaactt
      361 ttattcgctt tcaggataaa cgaaatctga tatacattta tgctattgga cttattctga
      421 taggctttta ttggaagaat gcagggttta tttttccatc agaggatatt ggtgtagtta
      481 gtgggattct ggctctggtg ctgtataatt ttatttttga tctcaaggcg tactgggctt
      541 ataaatgcgt cacgaagaat atcgattttt cgtggtttaa gaaaaagcag aaccacaaaa
      601 tagaattatt tcttacacaa cctctggtgg caggatttct gtcgttaatc atgttgagtg
      661 caatgagttg ggggctatac cagcttctac cctcgttata tgcgctgttc ctgatttcgt
      721 tacttgggcc gttggtcatc tttctgctgt ttcggatgat ccgcaccagt tatgtcaagc
      781 aggtcgctat ttcagtagcg aaaaaagtaa aatataaaag tctgactcgc tatgtgctgc
      841 tttcggtgtg catctcaacg gttgttaacc tgcttactat cagcccgttg cgtaacagtg
      901 attcttttgt gacagagggg cagtggttaa cgtttaaatc gataattgca ttgctcattc
      961 tttgtggcgt agtgttggcg attaatctgt tttttctgcg cttctccaag cggtacgctt
     1021 ttctgggcag gctttttttg caggaaatcg atctgttttt ctccagtgaa aatgcgttgt
     1081 cgaccttttt tgccaagccg ctttggcttc ggttattcat attgctggtt attgaagtga
     1141 tgtggattac gctggtgtcg gtattggcaa cgcttgtgga atggcggatt tggtttgaag
     1201 cctatttttt actctgctat gtaccgtgct taatttacta ttttttctat tgtcgattcc
     1261 tctggcataa cgattttatg atggcatgtg acatgtattt ccgttggggg cattttaata
     1321 agtgaggaag tgataggaag tgaccagata atacatatat gttctgtact ctcttgcgca
     1381 ttttgattgt tgactgagta accagacagt tgatgtgcac gatttcccct cgccctaaca
     1441 gacgtgggcg ggggcacccg gtgtgtgcaa tatacgaaaa aaaagcccgt actttcgtac
     1501 gagctcttct ttaaatatgg cggtgagggg gggattgact cgcttcgctc gccctgcggg
     1561 cagcccgctc actgcgttca cggtctgtcc aactggctgt cgc
//
LOCUS       DACAPB010000344         1598 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.354, whole genome shotgun sequence.
ACCESSION   DACAPB010000344 DACAPB010000000
VERSION     DACAPB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.354"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..225
                     /locus_tag="IT904_004707"
     CDS             <1..225
                     /locus_tag="IT904_004707"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725196.1"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP3194364.1"
                     /translation="KLGNAAEAIARIRAQYEPAAAYIDTTDGISIEYPEWRFNLRTSN
                     TEPVVRLNVESRADTALMNAKTEEILALLK"
     gene            389..1555
                     /gene="ugd"
                     /locus_tag="IT904_004708"
     CDS             389..1555
                     /gene="ugd"
                     /locus_tag="IT904_004708"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAP3194365.1"
                     /translation="MKITISGTGYVGLSNGVLIAQNHEVVALDIVQAKVDMLNQKISP
                     IVDKEIQEYLAEKPLNFRATTDKHDAYRNADYVIIATPTDYDPKTNHFNTSTVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTRDIKERLGIDNVIFSPEFLREGRALYDNLHPSRIVI
                     GERSARAERFADLLKEGAIKQDIPTLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESQGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIAAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGIPVII
                     YEPVMQEDEFFNSRVVRDLDAFKQEADVIISNRMAEELADVADKVYTRDLFGND"
BASE COUNT          412 a          400 c          405 g          381 t
ORIGIN      
        1 aagctgggaa atgcggcgga ggcgatagcg cgcatccggg cgcagtatga gccggcggcc
       61 gcatacatcg acacaacgga cggtatcagt attgaatacc ctgagtggcg ctttaacctg
      121 cgcacgtcca acacggagcc ggtggtgcgt ctgaacgttg agtccagagc ggatactgcg
      181 ttaatgaatg caaaaaccga agagatttta gccctcctca agtaattcat gagccataac
      241 ggaagccata gggtcagatt tgaatcgtat ttttgacctt atggcttatt cttgacatct
      301 cacaggtcaa agcgtaaaac cgcccacagc attattaaaa ttcgtggcta tccgccatta
      361 cccagttaac tgattaaagt gtaatctcat gaaaattact atttccggta caggttatgt
      421 tggtttatcg aacggtgtcc tgattgcgca aaaccacgaa gtggttgccc tggatatcgt
      481 acaggccaaa gtggatatgc ttaaccagaa gatctctccg attgtcgata aagaaattca
      541 ggaatatctg gcagaaaaac cgttaaattt ccgtgccacc acggataagc acgacgcgta
      601 ccgcaatgcc gactacgtga tcattgccac gccgaccgat tacgatccca agactaacca
      661 cttcaacacc tctacggtgg aagcggttat tcgcgatgtc acagagatca acccgaacgc
      721 ggtgatgatc attaaatcga ccatcccggt ggggttcacc cgcgacatca aagaacgttt
      781 agggattgat aatgttattt tctctcctga gttcctgcgt gaaggccgtg cgctgtacga
      841 taacctgcac ccgtcgcgca ttgtcattgg tgagcgctct gcacgtgccg aacgtttcgc
      901 agacctgttg aaagaaggcg cgattaagca ggatatcccg accctgttta ccgactccac
      961 tgaagcggaa gcgatcaaac tgttcgcgaa cacctatctg gcgctgcgcg ttgcctattt
     1021 caacgagctt gacagctatg ctgaaagcca ggggctgaac agcaagcaga ttatcgaagg
     1081 ggtatgcctg gatccgcgta tcggcaacca ctataacaac ccgtcctttg gctatggcgg
     1141 ctactgcctg ccgaaagata ccaagcagtt gctggcgaac tacgaatcgg tcccgaacaa
     1201 tatcatcgcg gctatcgtag atgctaaccg tacccgtaaa gactttatcg cggattctat
     1261 tcttgcccgt aagccgaaag tggtgggtgt gtatcgcctg atcatgaaga gtggttcgga
     1321 caacttccgt gcctcttcta ttcagggcat tatgaagcgc atcaaggcaa aaggtattcc
     1381 ggttattatc tatgaaccgg tgatgcagga agatgagttc tttaactccc gcgtagtgcg
     1441 cgacctggat gccttcaaac aagaggcgga tgtgattatc tctaaccgta tggcggaaga
     1501 gctggcggat gtggcggaca aggtatacac ccgcgatctg tttggtaacg attaattctg
     1561 cctgtctgac actggcggct cctcgccgcc agtatgct
//
LOCUS       DACAPB010000345         1469 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.358, whole genome shotgun sequence.
ACCESSION   DACAPB010000345 DACAPB010000000
VERSION     DACAPB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.358"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            398..862
                     /locus_tag="IT904_004709"
     CDS             398..862
                     /locus_tag="IT904_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194366.1"
                     /translation="MNPIITTHQEIVIENSVRYIELLKSEATKILDEYWVTWKARNQL
                     ISQTTYANGGKFMPGRFAPVFKKVGSSQKLTIVWKDFSPRFKNKIEHHGVVVKPKLGG
                     YSVSCFKNALDWELEMILKTENKIKPIRELLAEYHQRKLADIKRLEKLKRLI"
     gene            862..1469
                     /locus_tag="IT904_004710"
                     /pseudo
     CDS             862..1469
                     /locus_tag="IT904_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883411.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          469 a          310 c          312 g          378 t
ORIGIN      
        1 agcgacatct ttcattggta tcttttttga aaaaagttac caatggggaa aaatcttcat
       61 ttgtaacttt tcccggaata gtgaccaatg gacaacagat tttcatcctc catcatttgt
      121 aactattacg caaaaagtta cttatgtata agggtaacta tttctggttc gcaaaaaagt
      181 tacttttcca tctccacggt tgcgagaagt ggttttacgg gagatggaac gcacactgtg
      241 cgggcctcag gcagaggttg ctggtcgtgc tcacaagaaa gttacttttt tgccgacgac
      301 atgagcacaa aaaaattttt aaaaaaatgc aaacaccctt gttagttctg aaaattcttt
      361 atggattcct gtagtctgtg agaatggagg agttgatatg aacccgataa ttacgacaca
      421 tcaggaaatc gttatcgaaa actcggttcg ctatatcgag ttactgaaat cagaagcgac
      481 aaaaattctg gatgaatact gggtgacctg gaaggcacga aatcagttga tatcccaaac
      541 cacgtatgca aacgggggga aattcatgcc cggtcgattc gcaccggtgt ttaaaaaggt
      601 cggctcatcg caaaagctga caattgtatg gaaagatttt tcgcccaggt tcaaaaacaa
      661 aattgaacat catggcgtgg ttgttaaacc taaactcgga ggatacagcg ttagttgttt
      721 taaaaatgcc ctagactggg aactggagat gatcctgaaa acagaaaaca aaattaaacc
      781 gatccgtgaa ctactcgcgg aataccatca acgaaaactg gccgatatca aaagactgga
      841 aaaactgaag aggttaatat aatggctgac aacgcatccc caaaaactga accgcgcaat
      901 caacgtaacg ctggcaataa ccgcccgtta gggatggtca tttcaaaatt aaagccactt
      961 tcaagtgagt tctatacgca ctttcatgaa aaaaaacaga gccttcctgc gccagtttaa
     1021 cgccatttcc aatgcaattt ataatatttt ccggctggta gaaggtgacc aggcgacaag
     1081 cactaaagtt cagaaatggc tctccggtgt ttacgatgaa ctgtcgggta atgtaaatac
     1141 tttcaaagag cagattgaca aaaatgtcgc cagactgcct ctggatgatt catcattcga
     1201 agggtttgac tatggtgaat ttgaaattcg ctggaaccac ccaatgacct ataaattgct
     1261 ggatattatc cagaccatca acgtcctgac tcgtcaggca catcagttgt ggttatatgg
     1321 tcagatatca caacaggttc atgaccgaat tatcctgcaa ctcctgagct cactgcgggt
     1381 tgcgatggac aacatcacta aaatactgaa cgctgaaaac cgcgtgggca gctacagttc
     1441 acttacaccg cttctcaacc cggtacgca
//
LOCUS       DACAPB010000346         1460 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.359, whole genome shotgun sequence.
ACCESSION   DACAPB010000346 DACAPB010000000
VERSION     DACAPB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.359"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
BASE COUNT          539 a          305 c          228 g          388 t
ORIGIN      
        1 tgaacaccga acggctcaaa acgacaagtt ttctcccctg gtgcgtggcg ctctgacggg
       61 gtttcctgtt tccaaacttc acatctcgcc aggacaaaaa aaaccgacct ggggtcggtg
      121 gcaagaatca gagtctatgg gaaatatttt agttatcgga tgctcaatat tcacaaacgc
      181 aaaaaatgcc tcagtgatgc cctaaaacaa cctttccaga aggagtccgc gctttacgat
      241 gcacggaaga gataaaaaac cccacaaaag tggggaacca agagaaacaa caattacagg
      301 ttgttggttg tgttcgaaca acgatgaagt agctacgcta aataataatt accgtacagg
      361 ccctacatat tcaacactaa atttaagatc ctgacgaagg atttataaat gcacgaaaac
      421 acatagacag acaaattcac taaattcata cacaaaaaca agtgtagttt tctaagtaaa
      481 ttagattcga caaccaatta gtttatccac atcgcattcc actcttgcta caccaatcac
      541 atttcttgat atgtaatagc agaaaaaata aaaccagaca ccaatagacc aaaaaacaaa
      601 cattcaacaa gaaacatttt gaacaatatc acaaaactgt cacaaccatc atcagactat
      661 tgaaaaaaac tggttgctgt tttataaaag cgactgtaac aacctccact ctatagttcc
      721 attagtttaa agtgtcacac aaactgagca tccatttgtt ttccttactt ttgcccctgt
      781 attccaaggg gtttttttat aacttaaaga ttagcacacc acacaaaaac aataaaaaaa
      841 aacgacaagt tatacaataa aaccagtact atccatacag ctaattacat ataaatacta
      901 ttaaaccatc tggacattaa aaataaactc ttataacata aaattaatgc ggttccggta
      961 ccatatgtca gggataatgt gcaaatggag gtatcggaac tgcatttcag ttcattatta
     1021 attaatgaca aaaaaaacct ctgcgagcgc agagggggta aggaatgttc ccggtcataa
     1081 tatgtcgata gtgtttatat acatacagaa caagaacaac aaccagccaa ctaataaata
     1141 ttttattttt tagagctgaa accttaaagc tcacttccat tccatacatt gttataaacc
     1201 ttatgttgaa aactgaaagt aagtacggca gacttattaa gttgcgtgca gtcttttcac
     1261 gttatacaaa caagaacacc tgcgaagtta tttctcctta catatccccc tatgccaacg
     1321 ggggattttt tctggttcac taacgcataa ataatagtga caccctatca ttcacaaacc
     1381 gtgataatat aaggattatc atcatggcaa aattaggtga aaacacccca catattatcg
     1441 atgctactgt agattttatg
//
LOCUS       DACAPB010000347         1441 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.360, whole genome shotgun sequence.
ACCESSION   DACAPB010000347 DACAPB010000000
VERSION     DACAPB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.360"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            88..1038
                     /locus_tag="IT904_004711"
     CDS             88..1038
                     /locus_tag="IT904_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase"
                     /protein_id="HAP3194367.1"
                     /translation="MHQNNLKNKLNEILPFIKDKNEVIFLDYPVYLNVGDLLIYHGTE
                     NFFKENNINVKLRRSNARYSVKEIEKYLNPNTVILMQGGGNFGDLYPEFQKFRQDVVS
                     SFPDTRIIILPQTAFFENEHELDKSVKAFGAHKDLIIFSRDTRSLSIFKKFTSQTFLM
                     PDMAHSLWGTLPKSQKSKGTLYLIRNDKEINKVQRRLLEHKKGDDKYVDWEDILTSKD
                     LLMRKLCRKLDGIGGVTNLSVLKNMSNVIWYNYTYKMVQRYALYFTSHEKVVTSRMHG
                     HILSCLLSLPNDVIDNAYGKNSGYFKEWTYDIDGTKLLEE"
     gene            1042..>1441
                     /locus_tag="IT904_004712"
     CDS             1042..>1441
                     /locus_tag="IT904_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP3194368.1"
                     /translation="MKKDLKSITQVLSVGFSNVLLTFFQAILLTRLLTIEDRGHVQLF
                     IVSTTIFSSFFISGAGNTIAYCVRDGKKKGYVAILATLSLFLLIGLVVKFLLSNQTYQ
                     LLFFITQVVMLCVMQLTIELLKIQGSLKYYK"
BASE COUNT          505 a          192 c          262 g          482 t
ORIGIN      
        1 atagtattgt taaaaaaata actaattcag cctttcatgt gctggattag ttattgaatt
       61 ctttatgtgt aaattatagg gtaactcatg catcagaata atttgaaaaa taaattaaat
      121 gaaattttgc catttattaa ggataagaac gaagtaatat tcttagatta tcctgtatat
      181 ttaaatgttg gggacttatt aatctaccat ggaacagaga atttctttaa agaaaataac
      241 attaatgtta aacttcggag aagcaatgct cgttattcag ttaaggaaat agaaaaatat
      301 cttaacccta atactgtaat tctaatgcag gggggcggga attttggtga tttatatcct
      361 gagtttcaaa aatttagaca agacgtggta agttcttttc ctgatacacg aatcattatt
      421 ttaccacaaa ctgcattttt cgaaaatgag cacgaattgg ataaatcagt aaaagcattt
      481 ggagcacata aagatcttat cattttctca cgcgatacta gaagtctttc aatttttaaa
      541 aaattcacgt ctcagacctt cttaatgcca gacatggctc acagcttatg gggtactttg
      601 ccgaagtctc aaaaaagcaa aggtacatta tatttaatta gaaatgataa agaaatcaat
      661 aaagtacagc gtaggcttct cgagcacaaa aaaggggatg ataaatatgt tgattgggaa
      721 gatattttga cttcgaaaga tttattaatg agaaaactct gcagaaaact tgatggaata
      781 ggtggggtta caaatctctc agtattaaag aatatgtcga atgttatatg gtataactat
      841 acgtacaaaa tggttcaacg ttatgcatta tactttacca gccatgaaaa ggtagttaca
      901 tcccgtatgc atggacacat tttatcttgc ttattatcat tacctaatga tgtgattgat
      961 aatgcttatg gaaagaacag tggttatttt aaagaatgga catatgatat tgatggaacg
     1021 aaactattag aagaataagt catgaagaaa gatttaaaga gtataactca agtattgagt
     1081 gtaggcttta gtaatgtgct attgactttc tttcaagcca tattacttac aaggctatta
     1141 actattgagg atcgtggcca tgtccagtta tttatagtta gcacaacaat attttcgtct
     1201 tttttcattt caggcgcagg aaacacaatt gcgtattgtg tccgagatgg gaagaaaaaa
     1261 ggatatgtag ccattttggc tacattatcg ttatttttat taatagggct tgtcgtaaaa
     1321 tttttattaa gtaatcagac atatcagctt ttatttttta ttacacaagt ggttatgctt
     1381 tgtgtgatgc aacttacaat agagttgtta aaaatccagg gtagtctgaa atactataag
     1441 t
//
LOCUS       DACAPB010000348         1421 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.361, whole genome shotgun sequence.
ACCESSION   DACAPB010000348 DACAPB010000000
VERSION     DACAPB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.361"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(1..517)
                     /locus_tag="IT904_004713"
                     /pseudo
     CDS             complement(1..517)
                     /locus_tag="IT904_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(548..874)
                     /locus_tag="IT904_004714"
     CDS             complement(548..874)
                     /locus_tag="IT904_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194369.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(874..1293)
                     /locus_tag="IT904_004715"
     CDS             complement(874..1293)
                     /locus_tag="IT904_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194370.1"
                     /translation="MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKG
                     DGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLG
                     LFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGVM"
     gene            complement(1327..1421)
                     /locus_tag="IT904_004716"
                     /pseudo
     CDS             complement(1327..1421)
                     /locus_tag="IT904_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          373 a          354 c          296 g          398 t
ORIGIN      
        1 gctcaccggt ggcggtttca accctgatga gtggcgtgaa gctccccggc tgataaatcg
       61 tctggatgcg ggttctgtcg ttctgtattg tggtcaggcg gtcgccgtcc cagccgtacc
      121 aggtcacttg cggtttccgt gacagcgaca tccagcccgt caggtcccgt tcacgtcgcc
      181 acacccgttt tgccaccctg cggcccagcg ggtcgtaaag atagcggctt tcgaccagcg
      241 gctctgcata ttgtgtccgc gtgtagtgca ccagccggtg ctgactgtcg taatggtacc
      301 ggtgggtgcg ctcatcatcc gtgcggataa ccccttccgg gatgaggtcg gttttctccg
      361 tcagcctgcc gtgacggtca taccggtaaa gatagtgcgc gtcacgggcg atacggttat
      421 ccggccacat gctgagggcg ctgtccgggt gcagctccgg gtcgggcagg cggttacctg
      481 ccgggtctgt ggcatacggg atatcaatgc ccaggttaca tataataata agagcaatta
      541 acttaattta tttgtttata atgcgcttac ttaatacaaa ccattgaata taccctcctg
      601 acagaaaata aagccatatt attatcgcca taatatactt actatcaatc cttacgccaa
      661 taatgtctga tacagtccct ataaatatta gagcaataaa gaatactatg cctgttggaa
      721 aacatattac catcattcca taagacatga gactaattaa ctcgctcaca tcacgagtgg
      781 agtctaatag tgaaagtcta tgcatggaaa agaatagtat taaaagcgat aaagccagcc
      841 atatagctct gaataaaaaa ataatatatt tcattacatc actccggtga atacaaatct
      901 aataaaacgt ccaatgttat ttccggagca cttcatctta catgtttcta aagaatcatt
      961 agaccagtct tggcagtttt taaatagccc taaatttata ggtgtgaata tatcaggcaa
     1021 tacagaataa cgcggcctta cctctttaaa cttccctttt aaacagttct ccacacaact
     1081 attatcggtt tcaacgaggt cacaacggcc agcagcttct tttgtatcat tacttgcttt
     1141 acctggcccc catagaccat ctccttttga ttctccccgt tggtcttggc caccacaagt
     1201 atagtcccct ttaccatctg ggacacaaag atattgatga tagaacggat tcccccatat
     1261 atctggaccg cttctttcac cagttccgcc cattgaatgc agtggcttaa tgcattttag
     1321 tgtttctaat cctaatggat ctatatttga gatcggattc aacggatact gataaaaatt
     1381 ccatccccct ttcagcccaa tcggatcctg agtgatatat c
//
LOCUS       DACAPB010000349         1350 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.363, whole genome shotgun sequence.
ACCESSION   DACAPB010000349 DACAPB010000000
VERSION     DACAPB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.363"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>1350
                     /locus_tag="IT904_004717"
     CDS             <1..>1350
                     /locus_tag="IT904_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HAP3194371.1"
                     /translation="LPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAAN
                     MKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQL
                     GGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYDI
                     IAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVKI
                     SIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINTL
                     TMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLSA
                     VFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEGK
                     KGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD"
BASE COUNT          284 a          341 c          390 g          335 t
ORIGIN      
        1 tgctgccgca agaagttcag cagcaagggg tgagcgttga gaaatcatcc agcagcttcc
       61 tgatggttgt cggcgttatc aacaccgatg gcaccatgac gcaggaggat atctctgact
      121 acgtagcggc gaatatgaaa gatgccatca gccgtacgtc gggcgtgggt gatgttcagt
      181 tgttcggttc acagtacgcg atgcgtatct ggatgaaccc gaatgagctg aacaaattcc
      241 agctaacgcc ggttgatgtc attaccgcca tcaaagcgca gaacgcccag gttgcggcgg
      301 gtcagctcgg tggtacgccg ccggtgaaag gccaacagct taacgcctct attattgctc
      361 agacgcgtct gacctctact gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg
      421 gttcccgcgt actgctgcgt gatgtggcga agattgagct gggtggtgag aactacgaca
      481 tcatcgcaga gtttaatggc caaccggctt ccggtctggg gatcaagctg gcgaccggtg
      541 caaacgcgct ggataccgct gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct
      601 tcccgtcggg tctgaaaatt gtttacccgt acgacaccac gccgttcgtg aaaatctcta
      661 ttcacgaagt ggttaaaacg ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc
      721 tgttcctgca gaacttccgc gcgacgttga ttccgaccat tgccgtaccg gtggtattgc
      781 tcgggacctt tgccgtcctt gccgcctttg gcttctcgat aaacacgcta acaatgttcg
      841 ggatggtgct cgccatcggc ctgttggtgg atgacgccat cgttgtggta gaaaacgttg
      901 agcgtgttat ggcggaagaa ggtttgccgc caaaagaagc tacccgtaag tcgatggggc
      961 agattcaggg cgctctggtc ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg
     1021 ccttctttgg cggttctact ggtgctatct atcgtcagtt ctctattacc attgtttcag
     1081 caatggcgct gtcggtactg gtagcgttga tcctgactcc ggctctttgt gccaccatgc
     1141 tgaaaccgat tgccaaaggc gatcacgggg aaggtaaaaa aggcttcttc ggctggttta
     1201 accgcatgtt cgagaagagc acgcaccact acaccgacag cgtaggcggt attctgcgca
     1261 gtacagggcg ttacctggtg ctgtatctga tcatcgtggt cggcatggcc tatctgttcg
     1321 tgcgtctgcc aagctccttc ttgccagatg
//
LOCUS       DACAPB010000350         1336 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.364, whole genome shotgun sequence.
ACCESSION   DACAPB010000350 DACAPB010000000
VERSION     DACAPB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.364"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..995
                     /locus_tag="IT904_004718"
     CDS             <1..995
                     /locus_tag="IT904_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HAP3194372.1"
                     /translation="LWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAM
                     INPANGNTKPMFVGQGDQIFMNDVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADI
                     SGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRI
                     SDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQ
                     GVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            1062..>1336
                     /locus_tag="IT904_004719"
     CDS             1062..>1336
                     /locus_tag="IT904_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP3194373.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSV"
BASE COUNT          371 a          309 c          355 g          301 t
ORIGIN      
        1 agctgtggac gcagattgat aacggtcagc ttgcgcctga cctggctgaa atcaggacat
       61 ccattacgga tgtcagcaat gaaatcacac agaccgtcaa taagaaactg gaagaccaga
      121 gtgcggcaat tcagcagata cagaaggttc aggttgatac aaataataac ctgaacagca
      181 tgtgggctgt gaagctgcag cagatgcagg acggacgcct ttatattgcg ggtatcggtg
      241 ccggtattga gaatacgcca gcaggaatgc agagtcaggt gctgctggcg gcagacagga
      301 ttgcgatgat taatcctgcg aatggcaaca caaagccgat gtttgttggt cagggtgatc
      361 agatattcat gaacgacgtg ttcctgaaat atctgacggc tcccaccatt accagcggcg
      421 gtaatcctcc tgcattttcc cttacaccgg acgggcgact gacggcgaaa aatgcggata
      481 tcagtggcag tgtgaatgcg aacgccggga cgctcaacaa tgtcacgatt aatgagaact
      541 gtcagattaa ggggaaactg tcagccaatc agattgaagg cgatattgtc aaaacggtca
      601 gcaagtcttt cccccgcacg aacagttatg ccagtggcac catcacggta agaatcagtg
      661 atgatcagaa atttgaccgg caggtcatga taccgccagt gttattccgc ggtggtaagc
      721 atgagaattt caacagtaat aaccaacagt catactggta ttcaacctgc cggttaagag
      781 tgacccgcaa tggtcaggag atttttaatc agtccacgac ggatgctcag ggcgtatttt
      841 cctccgttat agatatgcct gccggacagg ggacgctgac actgacattc accgtatctt
      901 catcaggagc gaataactgg acaccaacaa ccagtatcag cgatctgctg gttgtggtga
      961 tgaaaaaatc cacagcaggt atcagtatca gctgaatttt ataacccaga acgggcgtca
     1021 gaaatgacgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag
     1081 caattttgtc cgcagccatc tgtctggccg tatccggtgc gcctgcatgg gcgtctgaac
     1141 atcagtccac gctgagcgcg gggtatcttc atgcccggac gaacgttccc ggcagcgatg
     1201 atctgaacgg gattaacgtg aaataccgtt atgagtttac ggacacactg gggatggtga
     1261 cctcattcag ctatgcagga gacaagaatc gccagctgac ccgttacagc gatacccgct
     1321 ggcatgaaga ttccgt
//
LOCUS       DACAPB010000351         1325 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.365, whole genome shotgun sequence.
ACCESSION   DACAPB010000351 DACAPB010000000
VERSION     DACAPB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.365"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            154..501
                     /locus_tag="IT904_004720"
     CDS             154..501
                     /locus_tag="IT904_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAP3194374.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            491..853
                     /locus_tag="IT904_004721"
     CDS             491..853
                     /locus_tag="IT904_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAP3194375.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            850..>1325
                     /locus_tag="IT904_004722"
     CDS             850..>1325
                     /locus_tag="IT904_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP3194376.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPM"
BASE COUNT          296 a          322 c          362 g          345 t
ORIGIN      
        1 cgtccttcag ggcgtggagg atgtcaattt gcgctatgat acccatctga cctccttgta
       61 gcggatggca aaagcatatc aggaaacggg cggacgaaat tcttaaaaaa accgctaaag
      121 gtagagcgtt agtgaaattg caaaggagca acaatgaaaa tttcccgcct cggagaagcg
      181 cctgattacc gcttctcgct ggctaatgag cgtacttttc tggcgtggat ccgtactgca
      241 ctaggatttc tggcggcggg ggttgggctt gatcaacttg cgccagattt cgccacgccc
      301 gtcattcgcg aactgctggc gttgttgttg tgtctgtttt ccggcgggtt agcgatgtat
      361 ggctacttgc gctggttacg caatgaaaaa gcgatgcgtc tgaaagagga cctgccctac
      421 accaacagct tattaatcat tagcttaata ttgatggtcg tggcggtgat cgttatggga
      481 ctggtgttgt atgccggata gccgcaaagc cagacgcatt gccgacccag gactgcagcc
      541 ggagcgcaca tcactggcgt ggtttcgtac catgctgggc tacggcgcgt tgatggcgtt
      601 ggctatcaaa cacaactggc accaggcggg catgttattc tggatttcga ttggcattct
      661 cgccatcgtg gcgctgatcc tctggcacta cacccgtaat cgcaatttaa tggatgtcac
      721 gaatagcgat ttttcccaat ttcacgtagt ccgtgacaaa tttttgatct ccctcgcggt
      781 gttatctctc gcaatactgt ttgctgtaac gcatatacat caacttatcg tatttattga
      841 gagagtcgca tgacaggaag tcaggtcatt gatgctgaag aggacaggca taaactggtt
      901 gtagagtaca aggatgctct gcaacctgcc gatttttatc acaatttcaa acagcggggc
      961 atccgctccg tacagctcat tccctatctt gaatttgatg accggggcga tctgacggcc
     1021 gcttcggtga cagcagagct gtgggggaaa tttttaattg ccctgttcga gtgttgggtg
     1081 cgggcagata tcagtcgtat ctcgattgag ctttttgacg ccaccttaca aaaatggtgt
     1141 ggcagcgaaa acccgcaacc gcgccgtgac tgccaggcgt gcgactggca tcgcctgtgc
     1201 ccgcacgctc ggcaggagac gccagacagc gtgctttgtg cgggctatca ggcgttttat
     1261 tcttactccg caccgcatat gcgggtgatg cgtgatctga taaagcagca tcgttctccc
     1321 atgga
//
LOCUS       DACAPB010000352         1299 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.366, whole genome shotgun sequence.
ACCESSION   DACAPB010000352 DACAPB010000000
VERSION     DACAPB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.366"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            19..>1299
                     /locus_tag="IT904_004723"
     CDS             19..>1299
                     /locus_tag="IT904_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HAP3194377.1"
                     /translation="MSGDLDILKETPSIRLKSAKGTAHLWFMNNDGSERGVVWSPENN
                     ESLGEIHIRAKNTKGESSGDFIVRHDGRVEARNLKITYKISAATAEFANTSTSSDNTT
                     VSIKGSQHTPLVLTSNNTIKNLSIGFKVDDVDQKYLGIAGDGDLYFGSYSDHTKNSKV
                     ITQAKLDSGVTVGGKTTFSDLATFNAGMAGSIEPETIDNKTIDLNDLIIANTVAGSVK
                     YYQCKTVAGGAYITNKPDGVSGNFLLRVESTRKTTGSDYAIMQTLIGSDTKRIYVRFV
                     VNGSWTEWSQVVVSGWNQDVTVRSLTSTTPSKLGGGRVDVLGSTSDYSSMNCAVRGVD
                     STGTNSAWSVGTSKNTGKMLCLKNHRSSAQVLLNGDDGAVQLLSGTVNGATAQALTIN
                     KDEVNSTADLVIRKQTGTGNRFALLNSGNSELPVG"
BASE COUNT          406 a          260 c          316 g          317 t
ORIGIN      
        1 aaaaaatccg gcgatgttat gtctggtgat cttgatatat tgaaagaaac gccatctatc
       61 aggctaaaat cagcaaaagg aaccgctcat ctgtggttca tgaacaacga cggaagcgag
      121 cgcggcgttg tttggtcgcc tgaaaacaac gaatcacttg gcgaaatcca catcagggcg
      181 aaaaacacaa aaggtgaatc aagtggtgat tttattgttc gccacgacgg gagggttgag
      241 gcccgcaatc taaaaataac ttacaaaatc agcgcagcca ccgcagaatt tgcaaacaca
      301 agcaccagtt ccgataacac tacggtaagc atcaaaggat ctcagcatac gcctttggtt
      361 ttaacgagca acaacacaat taaaaacttg tccattgggt ttaaggttga tgatgttgat
      421 caaaaatacc taggtatagc tggtgacggt gatttgtatt ttggtagtta ttctgaccac
      481 acaaaaaaca gcaaagtaat cacacaagca aaactcgata gcggggtgac ggtaggcggt
      541 aaaacaacct tttctgacct tgccacattt aacgcaggta tggcgggatc tatcgagccg
      601 gaaaccattg acaacaagac tattgattta aacgacttga tcattgctaa tacagtggct
      661 ggatctgtta aatactatca atgcaaaact gtcgcaggtg gtgcatatat taccaataag
      721 cctgacggcg taagcggtaa ctttttgcta cgtgtagaat ctactcgtaa aactacgggt
      781 tcagattatg cgatcatgca aacgctgatt ggcagcgaca caaaacgcat atacgttcgc
      841 tttgttgtca atggaagttg gacggagtgg agtcaggtag ttgtttcagg atggaatcag
      901 gatgtaaccg tcaggtcgtt aacctcgacg actccatcaa aattaggcgg cgggcgtgtt
      961 gatgtgctgg ggagtacgtc agattacagt agtatgaatt gtgctgtgcg cggtgttgat
     1021 agcactggaa ccaattcggc gtggtcagta ggtacatcga aaaacacggg aaaaatgttg
     1081 tgccttaaaa accacagaag cagcgctcaa gtgctgttaa atggcgatga tggcgcggtg
     1141 caactactaa gcggtactgt caacggtgct acagcacagg cgctaaccat caacaaagat
     1201 gaggttaact caactgccga tttagtaatt agaaaacaaa cagggactgg caatcgtttt
     1261 gctttactta attcaggtaa ttcagaacta ccagttggt
//
LOCUS       DACAPB010000353         1290 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.367, whole genome shotgun sequence.
ACCESSION   DACAPB010000353 DACAPB010000000
VERSION     DACAPB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.367"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..534
                     /gene="sitA"
                     /locus_tag="IT904_004724"
     CDS             <1..534
                     /gene="sitA"
                     /locus_tag="IT904_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAP3194378.1"
                     /translation="PHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQRNADTYKAKIT
                     QTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKV
                     VDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLK
                     VTTSTLVQGIKAGKREK"
     gene            534..>1290
                     /gene="sitB"
                     /locus_tag="IT904_004725"
     CDS             534..>1290
                     /gene="sitB"
                     /locus_tag="IT904_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAP3194379.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEE"
BASE COUNT          335 a          303 c          347 g          305 t
ORIGIN      
        1 ccccatgcct ggatgtcgcc agataatgct ctgatttacg tcgataatat tcgtgatgcg
       61 tttataaaat acgatccggc aaatgcacaa acctaccaac gtaatgccga tacttataaa
      121 gccaagatta cccaaaccct tgcccccctg cgtaagcaga ttgcggaact ccctgagaat
      181 cagcgatgga tggtcaccag tgaaggggct ttttcttatc tcgcacgcga tttggggcta
      241 aaagagcttt atctgtggcc gattaatgcc gatcaacaag gaacaccgca gcaggtacgt
      301 aaggttgttg atatagttaa gaaaaatcat atcccggcag tctttagcga gagtacgatt
      361 tccgataaac cagcgcgtca ggttgcgcgt gaaaccggcg cgcactacgg tggtgtgctc
      421 tatgtcgatt ccctgagcac agaaaacggc ccggtaccta cgtatatcga ccttctgaag
      481 gttactacca gtacgctggt acagggaatt aaagccggaa aaagggagaa ataatgatgc
      541 aatctgcagg cattgtcgtt aatgatgtta ctgtcacctg gcgtaacggg cacacagcac
      601 tgcgcgatgc atccttcaca gtaccaggcg gatctattgc cgctctggtt ggggtaaacg
      661 gttccggaaa atcgacgttg tttaaagcga ttatgggatt tgtgcgtctg accagcggga
      721 agatatctgt tctgggtatt cccacacgac aggcgctaca gaaaaacctg gttgcctacg
      781 tccctcagtc agaagaggtt gactggtcat ttcctgtact ggtggaagat gtggtgatga
      841 tgggccgcta tgggcatatg ggtatgttgc gtatcgccaa aaagagggat cgtcagattg
      901 tcaccgatgc gctggaacgt gtcgatatgg tggattttcg ccatcgacaa atcggcgagc
      961 tttccggtgg acaaaagaaa cgcgtctttc tggcgagagc gattgcacag cagggtgatg
     1021 tgatcctgct ggatgaacct tttaccggcg ttgatgtgaa aaccgaagcg aaaatcatca
     1081 gcctgttgag ggagttacgc gcggaaggca aaacgatgct tgtctcaacg cacaatctcg
     1141 gatccgtcac gacattttgc gattatacgg tcatggtcaa aggcaccgtc ctggcaagcg
     1201 ggccgacaga caccactttt acagccgaga acctggagct ggcttttagc ggcgtactgc
     1261 gccatgtcac acttaacggc tcggaagaaa
//
LOCUS       DACAPB010000354         1286 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.368, whole genome shotgun sequence.
ACCESSION   DACAPB010000354 DACAPB010000000
VERSION     DACAPB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.368"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            133..1269
                     /locus_tag="IT904_004726"
                     /pseudo
     CDS             133..1269
                     /locus_tag="IT904_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          421 a          229 c          283 g          353 t
ORIGIN      
        1 cacgtaacgc tacttttttg tactaaataa ttcgcatttt atgtttaaaa attgagatat
       61 tccttattac ctaaagctgt ttttcatagc ttatacatga tcaaatactc cttacataaa
      121 taaggtaaac aaatggaact taaaaaattg atggaacata tttctattgt tcccgattac
      181 agataagcct ggaaagtaga gcataaattg tcaggcatcc tactattgac tatttttgcc
      241 gttatttctg gtgcagaaag ttgggaagat atagaggatt tcggggaaac acatctcgat
      301 ttcttgaagc aatatggtga ttttgaaaat ggtattcctg ttcacgatac tattgccaga
      361 gttgtatcct gtatcagtcc tgcaaaattt cacgagtgct ttattaactg gatgcgtgat
      421 tgccattcat caaatgataa agacgtcatt gcaattgatg gaaaaacgct ccggcactct
      481 tatgacaaga gtcgccgcag gggagcgatt catgtcatta gtgcgttctc aacaatgcac
      541 agtctggtca tcgggcagat caagacggat aagaaatcca atgagatcac agctatccct
      601 gaacttctta acatgctgga tattaaagga aaaatcatca caactgatgc gatgggttgc
      661 cagaaagata ttgcagagaa gatacaaaaa cagggaggtg attatttatt cgctgtaaaa
      721 ggaaaccagg ggcggcttaa taaagccttt gaggaaaaat ttccgctgaa agaattaaat
      781 aatccagagc atgacagtta cgcaatgagt gaaaagagtc acggcagaga agaaatccgt
      841 cttcatattg tttgcgatgt ccctgatgaa cttattgatt tcacgtttga atggaaagga
      901 ctgaagaaat tatgcgtggc agtctccttt cggtcaataa tagcagaaca aaagaaagag
      961 ccagaaatga cggtcagata ttatatcagt tctgctgatt taaccgcaga aaagttcgcc
     1021 acagcaatcc gaaaccactg gcacgtggag aataagctgc actggcgtct ggacgtggta
     1081 atgaatgaag acgactgcaa aataagaaga ggaaacgccg cagaattatt ttcagggata
     1141 cggcacatcg ctattaatat tttaacgaat gataaggtat tcaaggcagg gttaagacgt
     1201 aagatgcgaa aagcagccat ggatagaaac tatctcgcgt cagtccttgc ggggagcggg
     1261 ctttcgtaat ctttccctgc tttttg
//
LOCUS       DACAPB010000355         1268 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.369, whole genome shotgun sequence.
ACCESSION   DACAPB010000355 DACAPB010000000
VERSION     DACAPB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.369"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..419)
                     /locus_tag="IT904_004727"
     CDS             complement(<1..419)
                     /locus_tag="IT904_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="EngC; RsgA; CpgA; circularly permuted GTPase;
                     ribosome small subunit-dependent GTPase A; has the pattern
                     G4-G1-G3 as opposed to other GTPases; interacts strongly
                     with 30S ribosome which stimulates GTPase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase RsgA"
                     /protein_id="HAP3194380.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLN"
     gene            514..1059
                     /gene="orn"
                     /locus_tag="IT904_004728"
     CDS             514..1059
                     /gene="orn"
                     /locus_tag="IT904_004728"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAP3194381.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTSSGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT          322 a          309 c          347 g          290 t
ORIGIN      
        1 atattgagtg acagctccgg caaaatggcg gagacaatga caatctggtc gatgttggcg
       61 gcaataggtt ttacgccgtc gtagaaatcc ggacgcgtca acaccgaggt acgctcatgc
      121 accgcttcca cgatcccttt gacatttacg ccttccgccg ccggtttacc cggacgccag
      181 actacgcggt cgccggtcac cagcgaacgg atggtacggc gaatattgca gcggtgaacg
      241 tcgccatcgg cggattccac atcagcgtgc ataccaaagc ggctgatgac gataccttca
      301 tcaggctcgc caaacagatt gtcgtcgtag tcgggcttct ccttagacgt tttaagacga
      361 cgctggtgat tggcgttcac gcggcgctgc tggcctttgg agagtttatt tttactcaat
      421 cgtacagact cctggtcgcc cctgatgggc aaaacatcta tgatacacgc aattgtggat
      481 caatatagtc actgtgaatg ggtggaaaat agcatgagtg ccaatgaaaa caacctgatt
      541 tggatcgatc ttgagatgac cggtctggat cccgagcgcg atcgcattat tgagattgcc
      601 acgctggtga ccgatgccaa cctgaatatt ctggcagaag ggccgaccat tgcagtacat
      661 cagtccgatg aacagctggc gctgatggat gactggaacg tgcgcaccca tacctccagc
      721 gggctggtag agcgcgtgaa agcgagcacg atgggcgatc gggaagctga actggcaacg
      781 ctcgaattct taaaacagtg ggtgcctgcg ggaaaatcgc cgatttgcgg taacagcatc
      841 ggacaggacc gtcgtttcct gtttaaatac atgccggagc tggaagccta cttccactac
      901 cgttatctcg atgtcagcac cctgaaagag ctggcgcgcc gctggaagcc ggaaattctg
      961 gatggtttta ccaagcaggg gacgcatcag gcgatggatg atatccgtga atcggtggcg
     1021 gaactggctt actaccgcga gcattttatc aagctgtaaa atttttcctg gtcacgtaag
     1081 cgccggaatc cacttgcggc ggcgtgattt tgacgctaaa ttgaacgtct tgctgataat
     1141 ttcagcactt gagataaaaa cgcaaaaaaa acttttttgg ggggttgcag agggaaagat
     1201 ttctcgtata atgcgcctcc cgtaacgacg cagaaatgcg aaaattacga aagcaaaatt
     1261 aagtagta
//
LOCUS       DACAPB010000356         1253 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.370, whole genome shotgun sequence.
ACCESSION   DACAPB010000356 DACAPB010000000
VERSION     DACAPB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.370"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1253
                     /gene="pepA"
                     /locus_tag="IT904_004729"
     CDS             <1..1253
                     /gene="pepA"
                     /locus_tag="IT904_004729"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAP3194382.1"
                     /translation="QYKQVIQKTINTLNDTGSMEAVCFLTELHVKGRNNYWKVRQAVE
                     TAKETLYSFDQLKTNKSEPRRPLRKMVFNVPTRRELTSGERAIQHGLAIAAGIKAAKD
                     LGNMPPNICNAAYLASQARQLADSYSKNVITRVIGEQQMKELGMHSYLAVGQGSQNES
                     LMSVIEYKGNASEDARPIVLVGKGLTFDSGGISIKPSEGMDEMKYDMCGAAAVYGVMR
                     MVAELQLPINVIGVLAGCENMPGGRAYRPGDVLTTMSGQTVEVLNTDAEGRLVLCDVL
                     TYVERFEPEAVIDVATLTGACVIALGHHITGLMANHNPLAHELIAASEQSGDRAWRLP
                     LGDEYQEQLESNFADMANIGGRPGGAITAGCFLSRFTRKYNWAHLDIAGTAWRSGKAK
                     GATGRPVALLAQFLLNRAGFNGEE"
BASE COUNT          287 a          320 c          377 g          269 t
ORIGIN      
        1 gtcagtacaa gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcaatgg
       61 aagcggtctg ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc
      121 gtcaggctgt cgagacggca aaagagacgc tctacagttt cgatcagctg aaaacgaaca
      181 agagcgaacc gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac
      241 tgaccagcgg tgagcgcgcg atccagcacg gtctggcgat tgccgccggg attaaagcag
      301 caaaggatct tggcaatatg ccgccgaata tctgtaacgc cgcttacctc gcttcacaag
      361 cgcgccagct ggctgacagc tacagcaaga atgttatcac ccgcgttatc ggcgaacagc
      421 agatgaaaga gctggggatg cattcctatc tggcggtcgg tcagggttcg caaaacgaat
      481 cgctgatgtc ggtgattgag tacaaaggca acgcgtcgga agatgcgcgc ccaatcgtgc
      541 tggtgggtaa aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca
      601 tggatgagat gaagtacgat atgtgcggcg cggcggcggt ttacggcgtg atgcgtatgg
      661 tcgcggagct acaactgccg attaatgtta tcggcgtgct ggcaggttgt gaaaacatgc
      721 caggcggtcg cgcctatcgt ccgggcgatg tgttaaccac catgtctggt caaaccgttg
      781 aagtgctcaa caccgacgct gaaggccgcc tggtactgtg cgacgtgtta acttacgttg
      841 agcgttttga gccggaagcg gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg
      901 cgctgggtca tcacattact ggtctgatgg cgaaccacaa tccgctggcc catgaactga
      961 ttgccgcgtc tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc
     1021 aggaacaact ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg
     1081 cgattaccgc aggttgcttc ctgtcacgct ttacccgtaa gtacaactgg gcgcacctgg
     1141 atatcgccgg taccgcctgg cgttctggta aagcgaaagg cgcaaccggt cgtccggtag
     1201 cgttgctggc acagttcctg ttaaaccgcg ccgggtttaa cggcgaagag taa
//
LOCUS       DACAPB010000357         1244 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.371, whole genome shotgun sequence.
ACCESSION   DACAPB010000357 DACAPB010000000
VERSION     DACAPB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.371"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>1244
                     /gene="mukB"
                     /locus_tag="IT904_004730"
     CDS             <1..>1244
                     /gene="mukB"
                     /locus_tag="IT904_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAP3194383.1"
                     /translation="GLHGKLKAGVCYSMLDTINSRHQRVVVGVRLQQVAGRDRKVDIK
                     PFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAMEGVQFKQFNSITDYHSL
                     MFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLRDYLLPENSGVRKAFQDM
                     EAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMRHANERRVHLDKALEFRR
                     ELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAASDHLNLVQTALRQQEKIE
                     RYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEVDELKSQLADYQQALDVQ
                     QTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAKELEATEKMLSLEQKMSM
                     AQTAHSQFEQAYQLVVAINGPLA"
BASE COUNT          304 a          345 c          367 g          228 t
ORIGIN      
        1 ggtctgcacg gtaagctgaa agcgggtgtc tgttattcga tgctcgacac cattaactcg
       61 cgccaccagc gcgtggtggt cggtgtgcgt ctgcaacagg ttgccggacg cgatcgtaaa
      121 gtggatatca aaccatttgc cattcaggga ctgccgatgt cggtgcagcc gacacagctg
      181 gtgaccgaaa ccctgaacga acgccaggcg cgcgtgctgc cgcttaacga gctgaaagac
      241 aagctcgagg cgatggaagg cgtgcagttt aaacagttca actccattac cgattaccac
      301 tcgctgatgt tcgatctggg catcatcgcg cgtcgtctgc gctccgcatc tgaccgtagc
      361 aaattctatc gtctgatcga agcttcgctg tatggcggga tctccagtgc cattacccgt
      421 tctctgcgcg actacctgtt gccggaaaac agcggcgtgc gtaaagcgtt ccaggacatg
      481 gaagcggcgc tgcgtgaaaa ccgcatgacg ctggaagcga ttcgtgtcac ccagtcggac
      541 cgtgacctgt ttaagcatct gatcagcgaa gctaccaact acgtggcggc ggactacatg
      601 cgtcacgcca acgagcgccg tgtccatctc gacaaagccc tggagtttcg tcgcgagtta
      661 catacttcgc gtcagcaact ggcggctgag cagtacaaac acgtcgatat ggcgcgtgag
      721 ctggcagagc acaacggtgc cgaaggcgat ctggaagcgg attatcaggc ggccagcgat
      781 cacctgaacc tggtgcaaac cgcactgcgt cagcaggaga agatcgaacg ctacgaagcg
      841 gatctcgatg agctgcagat ccgtctggaa gagcaaaacg aagtggtggc agaagccatc
      901 gaacgccagg aagagaacga ggctcgcgcg gaagctgccg aactggaagt ggacgagctg
      961 aaaagccagc ttgctgacta ccagcaggcg ctggacgtcc agcaaacgcg cgcgatccag
     1021 tataaccagg cgattgctgc acttaatcgt gccaaagaac tgtgccatct gccggactta
     1081 accgccgaca gcgccgccga atggctggaa accttccagg cgaaagagct ggaagcgact
     1141 gaaaaaatgc tctctcttga gcagaaaatg agcatggcgc aaaccgcgca cagccagttt
     1201 gagcaggctt atcagctggt ggtggcaatc aacggcccac tggc
//
LOCUS       DACAPB010000358         1244 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.372, whole genome shotgun sequence.
ACCESSION   DACAPB010000358 DACAPB010000000
VERSION     DACAPB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.372"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(24..308)
                     /gene="tnpA"
                     /locus_tag="IT904_004731"
                     /pseudo
     CDS             complement(24..308)
                     /gene="tnpA"
                     /locus_tag="IT904_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            316..>1244
                     /locus_tag="IT904_004732"
     CDS             316..>1244
                     /locus_tag="IT904_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3194384.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHR"
BASE COUNT          350 a          299 c          309 g          286 t
ORIGIN      
        1 tctggtcgat ctcgtcgcag tcacctaccc gatacgcctt tgaggctgtt taccagactg
       61 gatatcgcca gtttgggagg atagttaatt aacaaatgga cgtgatctgg ttcgccatcc
      121 atttcaacca gttcagcttc aaaatcagca catacatttg aaaagtaagc gcgtagtttt
      181 tctgtcgcgt catggtcaaa aatctgacgt cggtatctgg tgacaaagac caggtgaaca
      241 tgcatcagga aaacacaatg ccttccccgt cggatatcgg tttctttatt catagaccaa
      301 agtatcattt ggcctgtgaa acgattacag gcatttaaat tccagttaag accaggtggt
      361 caacaggagt gtgaaatgag gcgcttcgca ggcgcatgtc gtttcgtttt caatcgtgct
      421 ctggcacgtc agaatgagaa tcatgaggcc ggtaataaat acatccctta cgggaaaatg
      481 gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc aatggcttaa agattctccc
      541 tcacagccat tgcaacagtc actgaaagac cttgagcggg cttacaaaaa cttcttccgg
      601 aagcgggctg cttttccccg attcaaaaag cggggacaga atgatgcatt ccgctacccg
      661 cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact gggctggatg
      721 cgctaccgga atagccgtca ggtcacgggt gttgtgaaaa atgtcactgt cagccagtcc
      781 tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatccactcc ggctcaccct
      841 tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcgccacgct gtcagatggc
      901 acagtctttg agcctgtaaa cagttttcag aaaaaccaga agacgctggc gagacttcag
      961 cgacagttaa gccgcaaggt caaattcagc aacaactggc agaagcagaa acgcaaaata
     1021 cagcgactgc attcctgtat cgcaaatatc cgcagggact accttcacaa agtcacaacg
     1081 gccgtcagca aaaaccacgc aatgatagtc attgaggatt tgaaggtcag caacatgtca
     1141 aagtcagcag cgggtacggt cagccagccg gggcgcaatg tccgggcaaa atcaggttta
     1201 aaccgttcga tactggatca gggctggtat gaaatgcacc gcca
//
LOCUS       DACAPB010000359         1216 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.373, whole genome shotgun sequence.
ACCESSION   DACAPB010000359 DACAPB010000000
VERSION     DACAPB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.373"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..1172
                     /gene="chiP"
                     /locus_tag="IT904_004733"
     CDS             <1..1172
                     /gene="chiP"
                     /locus_tag="IT904_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAP3194385.1"
                     /translation="SGYAADMFGLDIAAFTAIEMAENGDSSHPNEIAFSKSNKAYDED
                     WSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLLAPHWSFMPGTYQGAEAGANFD
                     YGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKVDYLHSLGAKYDFKNNFVLEAA
                     FGQAEGYIDQYFAKASYKFDIAASPLTTSYQFYGTRDKVDDRSVNDLYDGTAWLQALT
                     FGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYASSNGRLDIWWDNRSDFNANGEK
                     AVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQSNPDAYYDKNRTIEESAYSLDA
                     VYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNIFQDERDVKFMVIAPFTIF"
BASE COUNT          308 a          308 c          318 g          282 t
ORIGIN      
        1 agtctggtta tgctgctgat atgttcggcc ttgatattgc cgcgtttacg gcgattgaaa
       61 tggcggaaaa cggcgacagc tctcacccga acgaaatcgc gttttcaaaa agtaataaag
      121 cctatgacga agactggtcc ggcgacaaaa gcggtataag cctgtataaa gcagcggcca
      181 aatttaaata cggtccggtt tgggcgaggg caggttatat tcagccaacc ggtcagacgc
      241 tgttagcgcc tcactggagc tttatgccgg gtacttatca gggtgcggaa gccggagcga
      301 attttgatta cggcgatgcc ggtgcgttga gtttctccta catgtggacc aacgaataca
      361 aagcgccgtg gcatctggaa atggatgagt tttatcagaa cgataaaacc accaaagttg
      421 attatctgca ctcccttggg gcgaaatacg acttcaaaaa taacttcgta ctggaagcgg
      481 cttttggtca ggcggaaggg tatatcgatc aatattttgc caaagccagc tacaaatttg
      541 atatcgccgc tagcccgtta accaccagct accagttcta cggtacccgc gataaagttg
      601 acgatcgcag cgtcaacgac ctttatgacg gcaccgcctg gctgcaggcg ttgacctttg
      661 gttaccgggc ggctgacgta gtggatttgc gcctcgaagg cacctgggtt aaggctgacg
      721 gtcagcaggg atacttcctg caacgtatga ctccaaccta cgcttcctca aacggtcgcc
      781 tggatatctg gtgggacaac cgttctgact tcaacgccaa cggcgaaaaa gcagtcttct
      841 tcggtgcgat gtatgacctg aaaaactgga atcttccagg cttcgccatc ggcgcttcct
      901 acgtttacgc atgggatgct aaacctgcga cctggcagag caatccggat gcgtactacg
      961 acaaaaaccg gactattgaa gagtctgcat acagcctgga tgcggtctat accattcagg
     1021 acggtcgcgc caaaggcacg atgttcaaac tgcacttcac cgaatacgac aaccactccg
     1081 acatcccaag ctggggcggt ggttacggca acatcttcca ggatgagcgt gacgtaaaat
     1141 ttatggtaat cgcaccattc accatcttct gatgcccgac gcggcaggtt ttgtgcctgc
     1201 cgcacgtttg aggaat
//
LOCUS       DACAPB010000360         1202 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.374, whole genome shotgun sequence.
ACCESSION   DACAPB010000360 DACAPB010000000
VERSION     DACAPB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.374"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            399..>1202
                     /locus_tag="IT904_004734"
     CDS             399..>1202
                     /locus_tag="IT904_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HAP3194386.1"
                     /translation="MLEQVKDFLGDSYNPQTGEFNAEKARAEAEEHKDKFNIILCGAT
                     GVGKSSLVNAVFGEEIVKSGVGSPVTQHLEKITVPRKGLVLWDTKGIESKDYENTINQ
                     LKNDILHAFNDSSHEDDVPHLGWVCIDSSGARIEERDIEIIGILREKEIPVVVVFTKI
                     LGDESADFIEAAISEINAHHGDFINGRYVKVNSRERKILNISIPVSGLDDLVDISFKA
                     LPESKSRARQALKKAQMVKMEERLEAMKDGAKLIVHTSSVAAGVVGATPV"
BASE COUNT          378 a          147 c          283 g          394 t
ORIGIN      
        1 tatttttacc tgtctgctgc cgtgatcgcg atgaacatgt tttagcagtg cgcataaaat
       61 ggggttatgt taaatgtgat tgatatctat agatatcaga ctaactattt attttgtaca
      121 tggtgttaaa attggtaatg attagaatgg ctatgactta taaatgttta tcatgccatt
      181 ggtgtaaaac tattcctaaa tgtagtgatg ctttagttga tggagtgagc gcctttagtc
      241 ggtgcccaaa ttgcgattct attttggttg atgaaaaaaa agccaacatc attaactcgg
      301 ttattgcaaa tataatattc aaaataaaat cgtaaatgat tttattattt gtgtgatcgt
      361 ttgcgttttt gttggcttgt tattatgtgg aggttaaaat gttggagcaa gttaaagatt
      421 ttttaggtga ttcctataat ccacaaactg gtgagtttaa tgcagaaaag gcaagggcag
      481 aggctgagga acataaggac aaatttaata taattctttg tggagctaca ggagttggta
      541 aaagttcttt ggttaatgca gtttttggcg aggagattgt taagtcagga gtgggaagtc
      601 cagttaccca gcatttagaa aaaatcactg ttccaagaaa agggctggtg ttgtgggata
      661 caaaaggtat tgagtcaaaa gattatgaaa ataccataaa tcagttaaag aatgatattc
      721 ttcatgcatt caatgactca tctcatgaag acgatgtgcc tcatttaggg tgggtttgta
      781 ttgattcgtc aggtgcaagg atagaagaaa gagatattga gataattggt attttaagag
      841 aaaaagaaat acctgtagtt gttgtgttta caaaaatact tggcgatgag tctgccgatt
      901 ttatagaggc agccattagt gaaattaatg ctcatcatgg tgattttatt aatggtaggt
      961 atgtaaaagt aaattcaaga gagcggaaaa tacttaatat ttctatccct gttagtggtc
     1021 ttgatgattt agtagatatt agttttaagg cgttaccaga atctaagagt cgtgccaggc
     1081 aagccttaaa aaaggctcaa atggttaaaa tggaggagcg actggaggcc atgaaggatg
     1141 gtgcaaaatt aattgttcat acatcatctg tagctgctgg ggtggttggt gcaactcctg
     1201 tg
//
LOCUS       DACAPB010000361         1194 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.375, whole genome shotgun sequence.
ACCESSION   DACAPB010000361 DACAPB010000000
VERSION     DACAPB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.375"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..987)
                     /gene="glnS"
                     /locus_tag="IT904_004735"
     CDS             complement(<1..987)
                     /gene="glnS"
                     /locus_tag="IT904_004735"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAP3194387.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLE"
BASE COUNT          284 a          298 c          317 g          295 t
ORIGIN      
        1 ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa
       61 ctcacgaata gaagccgcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg
      121 cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcaact tacgcttgga
      181 catcacggtg tattccagat tcaggcgcga gaactcatac tggcgcgggt gaacaggaat
      241 cgtgatgttg tccagtaccc agtcgtacag acgacggttg tcctggaact caagcgtaca
      301 cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg
      361 gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatac ggtacagcac
      421 cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt
      481 accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct cttcaacgct
      541 gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt attcgcggat
      601 ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata
      661 ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc
      721 taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt ttaccgggtt
      781 agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag
      841 gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt
      901 gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt
      961 agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac
     1021 ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagtgaaa atcgaaaatc
     1081 tcactgagat tatggggatg attgatgaaa aaaaaagcgg cagagaagat ctccaccgct
     1141 tttgccagtc ctgcgtcatt gttggatgcg acgcttaagc gtcttatcca acct
//
LOCUS       DACAPB010000362         1175 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.377, whole genome shotgun sequence.
ACCESSION   DACAPB010000362 DACAPB010000000
VERSION     DACAPB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.377"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..361)
                     /locus_tag="IT904_004736"
     CDS             complement(<1..361)
                     /locus_tag="IT904_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HAP3194388.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQS"
     gene            complement(1118..>1175)
                     /locus_tag="IT904_004737"
     CDS             complement(1118..>1175)
                     /locus_tag="IT904_004737"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194389.1"
                     /translation="VELHDKVIGHYLNIKHYQ"
BASE COUNT          321 a          304 c          288 g          262 t
ORIGIN      
        1 ccgactgatt gttgatgata tggtcggcgg cgaggaccag catcagcggg tcgtcaccgt
       61 agcgggtggc ctgaagggcc gccagggcga tggccggcgc ggtgttgcgc ccgaccggct
      121 caagtataat gttattcgac agcttattga gctggcgcag ctgttcagcc accaggaagc
      181 ggtgctcttc gttacagatg accatcggtt catggatttc aaggcccgag agtcgggtga
      241 tagtttcctg cagcatagag ttctgcccga acagacggag gaactgtttc gggtaaagct
      301 cgcgagacat cggccagaga cgactgccgg taccgccagc cataattaca ggaagtaaca
      361 taatgagatc cttaaataaa taattgcttt attttactat caatcaatcg accgtcaggt
      421 ggcgtggcct ttttgaggca cgctaccgcc attggctgtc gcaaccaaaa tgcgtactgc
      481 tcccgtgctc caggctaagc gccaccctct ttcgcttcag gctgtcggca gatcgttcag
      541 tcagcagcta caatgacagc ctggcggaat gcgtgtcctg aaaacctaaa agcagagata
      601 taccttcagg catatctctg cttttaacat gtatcttccc tcacctagcc gccaaacgca
      661 ccacactttc cgcatttgcc gccgggttaa ccatcaggac tttctcaatt acgcagggca
      721 aaacgcatgc ccttaccgcc accgaaaaaa gcgcaacggg cgcccagccg atgccggcaa
      781 ccgtcagaaa ggagcgttta cgacgtgggg aaagataaga agagacatcg tcttcaccgc
      841 caaggagacc acgcgtattc aggtttgatg gccctatgca cacaacatcg gcgctatgtt
      901 ccgataggtg ggataaacga ctcatgcctc tacactgcac attccacaaa gaaaatcgtg
      961 cgcagattat agcgctggtc ggcgggaata aaaataaaaa agcctactga ttaatggatt
     1021 actcaaatga cacacaccat aaacattcat taaacaagca aaaaaagagc aatttgttga
     1081 tatcaatgaa gaaaagaaag ctggtaatga ctccaactta ttgatagtgt tttatgttca
     1141 gataatgccc gatgactttg tcatgcagct ccacc
//
LOCUS       DACAPB010000363         1163 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.378, whole genome shotgun sequence.
ACCESSION   DACAPB010000363 DACAPB010000000
VERSION     DACAPB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.378"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..454)
                     /locus_tag="IT904_004738"
     CDS             complement(<1..454)
                     /locus_tag="IT904_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP3194390.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKP"
     gene            complement(817..>1163)
                     /locus_tag="IT904_004739"
     CDS             complement(817..>1163)
                     /locus_tag="IT904_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP3194391.1"
                     /translation="DISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQQ
                     NVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSH
                     PVNTYTGVLLPK"
BASE COUNT          317 a          236 c          240 g          370 t
ORIGIN      
        1 ttggtttgcc tgcagcttcc atacgcttgc gccaacgcca tttcatcgcc atcccgccga
       61 gcatacaggc ctcggaagaa ccaatggtgt tggtgccaac ggcctgacca tttttcggcg
      121 caggcgcatg ccacagatcg gcaaccatat ttacgcaacg caggtcgatg gctgcggatt
      181 gcggatattc ttctttgtcg atccagtttt tgttaatgga taaatccatc aacttgtgga
      241 cattgtcgtc gtcccaggtc tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa
      301 gatataattc gtcattgata atctgaaatg cgacatcgtc gcgcatttcg tgcagcggaa
      361 aacgttttga ttctgcgata gtggaaatag acttcgcacc aaaacgtgaa tcgagtagtt
      421 ccgaccttaa atccgttact tgcttcttat ccattttaaa ctccttaaaa tgattggatc
      481 gcattaaaaa agtaggattt atcgataaag taagcaagtg caaaggactc gggtttaaat
      541 aacaaaatcc taatgttatt tatcatgaga tattacgcga ataatgtttt ttcattgaaa
      601 aaacaatata atagggggtt cttgggtggt ggtcaggtgt tttatgctgt tatttttatg
      661 cgcattcttt gcctcctgaa ttatcacgta aaaatcagac cttaaaatat cactattagt
      721 acttagttat tattttgaac gcatttataa aattattaca taaaaatggc gaatattgct
      781 aaaattcccg ccaacgatgt gttgacgggg ctgttattat tttggcaata atactccggt
      841 ataagtattt accggatgag aaagatattg tttaacggca gtgttaacat tttctaccgt
      901 catttgtttc aacaattgct cctgctcagt ccatgctgca ggatcgtcat attgaataag
      961 actatttaca atggtgttcg ctaattgttg aacgctacgc tgttggatat cgaggctgcg
     1021 ctgaacgttt tgctggtatt cattcagttc ttgctcactg atccctttag ccagacgctt
     1081 aaccatcact tcattcgcta acgttaacag ttcatcatgt cgttctggtt gacaagtaaa
     1141 agccagcaaa tgactgatat ctt
//
LOCUS       DACAPB010000364         1109 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.379, whole genome shotgun sequence.
ACCESSION   DACAPB010000364 DACAPB010000000
VERSION     DACAPB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.379"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            201..1022
                     /gene="lnu(F)"
                     /locus_tag="IT904_004740"
     CDS             201..1022
                     /gene="lnu(F)"
                     /locus_tag="IT904_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(F)"
                     /protein_id="HAP3194392.1"
                     /translation="MLQLKMIELFKEGCHEDARIIAALMFGSFAIGEGDEFSDIEFAV
                     FIQDDHFENFDQRSWLNAVSPVAAYFPDDFGHHTALFENGIRGEFHFMRKSDIPVIST
                     WQGYGWFPSLEAAVLLDRSGELSRYASALVGGPPIREGAPLVEGLVLNLISLMLFGAN
                     LLNRGEYARAWALLSKAHENLLKLVRLHEGATDHWPTPSRALEKDISEDSYNRYLACT
                     SSAEPRALCAAYHQTWTWSLELFKSVTEPLNIELPRTVIAQAKRLFNESATPHNK"
BASE COUNT          291 a          272 c          281 g          265 t
ORIGIN      
        1 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtaatc
       61 ggcaaataat gtctaacaat tcgttcaagc cgaccgcgct acgcgcggcg gcttaactcc
      121 ggcgttgtgc tgacgaaaaa aaacaaaaat ctgcgtatac ctttcctcat aatctgtggc
      181 ttcaataaaa ggatatttct atgcttcaac tgaaaatgat cgaactcttc aaggaaggtt
      241 gtcatgagga tgcacgaata atcgcggcat tgatgttcgg ctcatttgct atcggagagg
      301 gtgacgagtt ctctgatatc gaattcgcag tgttcatcca ggatgaccat tttgaaaatt
      361 tcgatcagcg ctcgtggctt aatgccgtaa gtccggttgc tgcttacttt ccggacgact
      421 tcggccacca caccgcactt tttgaaaacg gcattcgcgg tgaattccat ttcatgcgaa
      481 aatcggacat accggtcatt tccacttggc aaggctatgg gtggtttccc tcgcttgagg
      541 cggctgtttt gttggaccga tcaggagagt tgtcaaggta cgcaagcgct ctcgtgggcg
      601 gtcccccgat acgtgaaggc gcgccgctgg tggaagggct tgtgttgaac ctcatcagcc
      661 tgatgctctt tggggccaat cttttaaatc ggggagagta cgctcgcgcc tgggctttgc
      721 tcagcaaagc acatgaaaac ctactcaagc tggttcgact ccacgaaggg gcaacagacc
      781 actggccgac accttcacgc gcgctcgaaa aggatatctc ggaggactcg tataatcgct
      841 atctggcatg cacaagcagt gcagaaccaa gagcactatg tgcagcctat catcaaacgt
      901 ggacgtggag tctcgaattg ttcaagagcg tgacagaacc tctgaatatc gagcttccga
      961 gaactgtaat tgcgcaggca aaaaggttgt tcaatgagtc tgcgacgccg cacaacaagt
     1021 aaatccagcg gacgcataaa aacgcgccgc tgattttgac gttagatgca ctaagcacat
     1081 aattgctcac agccaaacta tcaggtcaa
//
LOCUS       DACAPB010000365         1099 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.380, whole genome shotgun sequence.
ACCESSION   DACAPB010000365 DACAPB010000000
VERSION     DACAPB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.380"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..554
                     /locus_tag="IT904_004741"
     CDS             <1..554
                     /locus_tag="IT904_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAP3194393.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYG
                     ARPNKPPTPDSSKLPDIAKQLRLPYPIDQASSAPNLFKTFFRALSPYDYTLYCRKWVK
                     PNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPAT
                     AGIDDLGEILAKMKQRTSRGIRK"
     gene            551..>1099
                     /locus_tag="IT904_004742"
     CDS             551..>1099
                     /locus_tag="IT904_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194394.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHEL"
BASE COUNT          369 a          204 c          238 g          288 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaatttttat cagtatccgc
       61 tgaatccggt tcagtatata gattcaatgg gactggcatc aaaatatgga cacttaaata
      121 atggcggata tggagcgaga cccaacaaac cgcctacgcc cgattcaagt aaattgccgg
      181 acatagcgaa acaattaaga ctgccatatc ctattgacca ggccagtagt gcgcctaatc
      241 ttttcaaaac attcttcaga gcattaagcc cttacgacta cacactgtat tgcaggaagt
      301 gggtaaaacc aaatctgact tgtacgccac aggatgattc ccagtatcca gggatggata
      361 caaagacagc aagtgattac ctgccacaga caaattggcc aacaactcaa ttaccaccag
      421 gatatacttg tgcagaaccc tatttattcc cagacattaa taaacccgat gggccagcaa
      481 cagcagggat agatgatttg ggtgaaattt tagctaagat gaaacagaga acatcgagag
      541 gaataagaaa atgaaaagag ttttgttctt tttgctgatg atatttgtta gttttggtgt
      601 tatagctgat tgcgaaatac aagctaaaga tcatgattgt tttactattt tcgctaaggg
      661 tactattttt tccgcgtttc ctgtcttaaa taataaagcc atgtggagat ggtatcagaa
      721 tgaggacatc ggtgagtatt attggcaaac agaactgggt acatgtaaaa ataataaatt
      781 cacaccaagc ggggcgagac tcttaattcg cgtcgggtca ctacgcctta atgaaaatca
      841 tgccatcaaa ggaacgttgc aggagttgat aaatacagca gaaaaaacgg cgtttctcgg
      901 cgatcggttt agaagttata taagagcggg tatctatcaa aaaaagagca gtgatccagt
      961 tcagttgttg gcagtccttg ataattcaat catggtaaaa tattttaaag atgagaaacc
     1021 aacttatgct cgaatgacag cacatctccc gaacaaaaat gagtcttacg aatgtttaat
     1081 aaagatacag catgaacta
//
LOCUS       DACAPB010000366         1086 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.381, whole genome shotgun sequence.
ACCESSION   DACAPB010000366 DACAPB010000000
VERSION     DACAPB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.381"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..110
                     /locus_tag="IT904_004743"
     CDS             <1..110
                     /locus_tag="IT904_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HAP3194395.1"
                     /translation="EEAINKRKHERELLNKVCIVSMLARLRLMPKGCAQ"
     gene            107..298
                     /locus_tag="IT904_004744"
     CDS             107..298
                     /locus_tag="IT904_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HAP3194396.1"
                     /translation="MNPVFALILTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGN
                     CYLVDKVIHQDNIEIPAGL"
     gene            392..>1086
                     /locus_tag="IT904_004745"
     CDS             392..>1086
                     /locus_tag="IT904_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP3194397.1"
                     /translation="MSKVFICAAIPGEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGK"
BASE COUNT          321 a          233 c          260 g          272 t
ORIGIN      
        1 ctgaagaggc cattaacaaa agaaaacacg aacgggagtt attaaataaa gtatgcattg
       61 tttcaatgct ggcccgttta cgtctgatgc caaaaggatg tgcacaatga atccagtatt
      121 tgcacttatt ctgacggttt ttcttgtttc cggagagcca gttgatattg cagtcagtgt
      181 tcacagaaca atgcaggaat gtatggcagc agcaaccgaa cagaaaattc ccggtaactg
      241 ttacctggtc gataaagtta ttcaccagga taatatcgaa atcccggcag gtctttaaaa
      301 cagttccgta ataaatatcc ggtttcattc ttatatgcca gcaatggcag ggatttgttc
      361 atccttaaat ctgtcatgag gttaaaacaa aatgagtaaa gtttttattt gcgccgccat
      421 tccgggcgaa caggcaataa aggaagaagg tgccgtcgct gtagccactg ccattgaagc
      481 cggtgatgaa cgtcgcgccc gcgcaaaatt tcactggcaa ttcctggaac attatccggc
      541 tgctcaggac tgcgcttata aatttattgt ctgcgaggat aaacctggca taccccgccc
      601 tgccctcgat tcatgggatg ctgaatatat gcaggaaaac cgctgggatg aggagtctgc
      661 ttcttttgtc ccggttgaga ctgaatccga tccgatgaac gtcacttttg acaagctggc
      721 ccctgaagta cagaacgctg tcatggttaa gttcgacaca tgtgaaaaca tcaccgttga
      781 tatggttatt agcgcacagg aattgttgca ggaagacatg gcaacattcg acggacatat
      841 cgttgaagcg ttgatgaaaa tgccagaagt taacgccatg tatccggagc ttaagttgca
      901 cgccattggg tgggttaagc ataaatgtat tcctggtgct aaatggcccg aaattcaggc
      961 agagatgcgc atctggaaaa aacgtcgcga aggtgaacgc aaggaaaccg gaaaatacac
     1021 gtctgttgtt gatctcgccc gcgccagagc caatcaacag tacactgaaa attcaacagg
     1081 aaaaat
//
LOCUS       DACAPB010000367         1075 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.382, whole genome shotgun sequence.
ACCESSION   DACAPB010000367 DACAPB010000000
VERSION     DACAPB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.382"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>1075
                     /locus_tag="IT904_004746"
     CDS             <1..>1075
                     /locus_tag="IT904_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAP3194398.1"
                     /translation="QLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRL
                     TSVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRYG
                     RLTEKTDLIPEGVIRTDDERTHQYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRV
                     AKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRV
                     ETATGELAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRR
                     WLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLSLALISKEGTTAWYAEYDEWGN
                     QLNEENPHQLQQLIRLPGQQYDE"
BASE COUNT          258 a          331 c          325 g          161 t
ORIGIN      
        1 ggcagttaca gagccagcac ctgaacagcc tgcagtatga ccgcgattac acctggaacg
       61 acaacggcga actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagca
      121 ccaccggcag gctgaccagc gttcacacca ccgcagcgaa tctggatatc cgcatcccgt
      181 atgccacaga cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcaccc
      241 tcagcatgtg gccggataac cgtatcgccc gtgacgcgca ctatctttac cggtatgacc
      301 gttacggcag gctgacagag aaaaccgacc tcatcccgga aggggttatc cgcacggatg
      361 atgagcgcac ccaccagtac cattacgaca gtcagcaccg gctggtgcac tacacgcgga
      421 cacaatatgc agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg
      481 tggcaaaacg ggtatggcga cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga
      541 aaccggaagt gacctggtac ggctgggacg gcgaccggct gaccacgata cagaacgaca
      601 gaacccgcat ccagacgatt tatcagccgg ggagcttcac gccactcatc agagttgaaa
      661 ccgccaccgg tgagctggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt
      721 ccggcggcga agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc
      781 ggctggaaag tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat
      841 cgtgcggcct gactgtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc
      901 gaaaaattca cctgtaccac tgcgaccatc gcggcctgtc gctggccctt atcagtaagg
      961 aagggacaac agcgtggtac gcagaatacg atgaatgggg taaccagctg aatgaagaga
     1021 acccgcacca gctgcagcag ctcatccgcc tgccggggca gcagtatgat gagga
//
LOCUS       DACAPB010000368         1067 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.383, whole genome shotgun sequence.
ACCESSION   DACAPB010000368 DACAPB010000000
VERSION     DACAPB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.383"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            300..404
                     /locus_tag="IT904_004747"
                     /pseudo
     CDS             300..404
                     /locus_tag="IT904_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            744..1001
                     /locus_tag="IT904_004748"
     CDS             744..1001
                     /locus_tag="IT904_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAP3194399.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          366 a          184 c          198 g          319 t
ORIGIN      
        1 tcccgaactc aaagctataa tacagataat tactggttaa ccctatatag ggcattttaa
       61 agtgttgtga tcttacccag caatagtgga cacgcggcta agtgagtaaa ctctcagtca
      121 gagatgactc acaagacaaa acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat
      181 gaatttcgta gttaagatct gaagcttgca gattgcatcg gtgttactgc cggagtccgt
      241 gaacttagtc tgcatgaatc accaaagctt tatatctgtc gcagtaaaca gcaaaatcag
      301 caaacttact taattatatc gagtgcgatt acaatcgctg gcgatggtac agttggtgtg
      361 gcggtctcag tccggaacaa tttgaaaatc agaatctcac ttaaagcggt atccatttta
      421 caaaagatgg ccgtctgtgt atttgtaatg aataccttct acatgtttag agtgcataat
      481 ccacgacaag gcgatatcct aaaaattagc tagcaggcaa ctttgatggc atagataatg
      541 tttttagtaa aaaagagttg actgaatata atacagcctc ttcctacata agaaggaatg
      601 tgctcacaaa aaaaaaccat tagtctgctt cctgttctta caataatcag tattatgtat
      661 tttatttata tagcaaaata tgcttgttac acctgtcata attaggcatg gtaaaattat
      721 tgactgaacc ctgggggaga aagatgcaca gtgattatta taatatggtt tttggtgaaa
      781 aattggccaa tattttatat gaagctaact ctcaattttt ttacgagaga aatgtcatag
      841 aagaggcagt caacgcttta ttctgtgagc gtgaaataat aaacaacaag aacataataa
      901 agaagctgat gttctttttg tcagatgtta atcacaccaa gaaagatgtt gttcagagcg
      961 cacttaatat tatcattgac attacatcag gtgatatttg agattttaca taaaagataa
     1021 tctctcaagg agtcttatct gaaaacgaaa tatatagtcc ctctcgt
//
LOCUS       DACAPB010000369         1052 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.385, whole genome shotgun sequence.
ACCESSION   DACAPB010000369 DACAPB010000000
VERSION     DACAPB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.385"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            424..928
                     /locus_tag="IT904_004749"
                     /pseudo
     CDS             424..928
                     /locus_tag="IT904_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966901.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT          355 a          205 c          189 g          303 t
ORIGIN      
        1 ttcccaccat ttaatatcac tgtgtagagt tgatatcact acaacagaaa catgcatgtc
       61 atttgtttac ttctcttttt accacagaaa aatttatcta tttttctaag catcagtgaa
      121 atactcataa aggtaactat tatgtctatg ccattaagca atgcccttca gagttaaatt
      181 attacatata atcacttcct tcatcatcca aaagtcgaca gtgagttaac acgtaaacat
      241 gaacgtgcac gcatggatac ggaaaatatc tatcttccgc ctcttgctcg tggcaataac
      301 cataattacg atggaaaaag cgttgtcgaa attagaaaac ttgatattag taaagagtcc
      361 tggccattta attatgtgac agaaacatgc agagaattca atggtattac aacaacagga
      421 cgtatgctct atcgaaactt gaaaatcacc tcagctttag acgaaatata tggtggtata
      481 tgtaaaaagg cgcatgctgc cacagagtta gcaaaaggat tacggctaaa tttatttatg
      541 aaaggccctt tcgatcctgt tgaggactat acagttcatg aaataacatt agaccctggt
      601 tgcaatgttc ctggttatgc cggaaccacg ataggatata tttcaaccct tccagcttct
      661 caggcaaaaa gatggaccaa tgagcagcca aggattgata tctatattga tcagataatg
      721 acggtaacag gggtagcaaa ttcttccgga ttcgcccttg ccgcattact taatgctaat
      781 attgagttgg gtaatgaccc aataattggt atagaagcct atcccgggtg atgaaaatgc
      841 accactcaaa cgtatgactc tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa
      901 tggtgaatgg aattatattg gtaaataact accaggaaga atgagtacta acattcaggt
      961 tatacagata gctaaacaat cccctgcaat ttacaaagta atactcattg cgacttctta
     1021 atttaagaaa gcgcaaggaa cgtttaccct tg
//
LOCUS       DACAPB010000370         1044 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.386, whole genome shotgun sequence.
ACCESSION   DACAPB010000370 DACAPB010000000
VERSION     DACAPB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.386"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..520
                     /locus_tag="IT904_004750"
     CDS             <1..520
                     /locus_tag="IT904_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAP3194400.1"
                     /translation="TATGIRNIAAFTNELSNLQSELANIYKQGNSYISDFTSNPEGAL
                     SSQAKNLFSKYGAFNMCSTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAMSQIQS
                     LSNRIESSKDMKESQDLANALQAQSLKIQAIKMQYDVWDSKNRADAEMLKEQKYAEYR
                     LRQKNAKIPSFE"
BASE COUNT          339 a          179 c          221 g          305 t
ORIGIN      
        1 cacggcaaca ggtataagga atattgcggc attcacaaat gaactaagta acctacaatc
       61 agagctggca aacatttata agcaggggaa cagttatatt tctgatttta ccagtaatcc
      121 tgaaggtgca ttaagtagtc aggcgaaaaa tctgttcagc aagtatggtg cttttaatat
      181 gtgttctaca ggcattgaaa gttctgataa cctttgtaag gccagaatag tttcaacggc
      241 cgccagtatt gaacagggaa atgagataaa taagcagcta tcaagtgcaa tgtctcagat
      301 tcagagttta agtaaccgaa ttgagtcttc aaaggatatg aaagaatccc aggatttggc
      361 taacgcattg caggcgcagt cgttaaaaat acaggcaatt aaaatgcagt atgatgtatg
      421 ggatagtaaa aacagggctg atgcggaaat gttgaaagag caaaagtatg ctgagtatcg
      481 cctgcgtcag aaaaatgcaa agataccgag tttcgaatga tatgataaag ctaaataagc
      541 taaaagaatg ggtagataat cattggtcta gattgaatag taccggttct acaccgccgc
      601 cactgttaat ctcgctacca atcatagtta ttgttttaag tgtggagctt acgttaaagc
      661 tggttaatgc tttaatgtat tatggtacat tgcaatttat cactgataaa attgaaaaga
      721 ggtttgttcc cagcggtggt ataaaagaaa gactcctttt tacgttaaag ctgttttgta
      781 ttttacttct gttattaata gtaatgcgat tttcaatgaa gtaaaaaata aaggtcatcg
      841 aatagatggc cttttctatt ctaaattcta aatatagagc ctgcggcagg cacaggcaaa
      901 aacatcgttt ttggacaatc tgattcaaat gttaacattt gacatcggat tgtctatcct
      961 gcattgctct tttgccgacc tcaaaacctc tagcgtgttt ttgtttcccg atctctagaa
     1021 ccaccgagcc gccacggttc cggc
//
LOCUS       DACAPB010000371         1029 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.387, whole genome shotgun sequence.
ACCESSION   DACAPB010000371 DACAPB010000000
VERSION     DACAPB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.387"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(1..358)
                     /locus_tag="IT904_004751"
                     /pseudo
     CDS             complement(1..358)
                     /locus_tag="IT904_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            428..670
                     /locus_tag="IT904_004752"
                     /pseudo
     CDS             428..670
                     /locus_tag="IT904_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
BASE COUNT          292 a          216 c          229 g          292 t
ORIGIN      
        1 gccaggtcag ccgggaaggt tgattgtctg atgattcaag caaacgatcc aaagcttccc
       61 tgtgagcaga ggttaagggc gcatttagcg tctgaaagac tattttatcg ccgccagcca
      121 tagcttccgc acaggtacgt tccacaacat caatcgcagg aaacataacc ttgttttgcc
      181 gaaggtaggc cagcatttct tccgccagga gaatgccttt gtcggtacga gtcgccattg
      241 gcaacagatg tgtaatacaa ctctgctgta ttttgttggt gaatgctttt acaccaatat
      301 aacggtacaa ctcggttaaa tgctcgcgtc gagtagtctc cctgccgttg atataagaga
      361 tccttcacaa tcagatccga ttacccgcgc agtgataacc gcaataaata atatgcttat
      421 agacctgatg gccgcgatgt cgcataagga ttggctgagt cgtcgccaac gccaaaaaca
      481 gggaattgaa tgagctcata cgttgggtaa ataccgggga aaacaagccg atcaggaacg
      541 gcatcagaaa gtgttgtact acagacaagt aaaaaaactc agcattagag aaacagcaga
      601 tgctacgggc tacagcactt cgcaggtttg ccgtattcag gcactttatc gagaaccaac
      661 gactgattaa aaagtagcct taacgtacaa taattttcga tatccaaact gacccctatc
      721 taaacttaac agcacttgtg gccagatgtg ctcggtagac atgtgactgg ttgagtctgg
      781 ctccgctttt tctggatact gaaacggcag gacaggggaa tactgcggag gccttggaaa
      841 tacatttttg tgtgtttaaa caagggatta tggtaaatgc tgcattgaaa attgaaatct
      901 tgcgcatgat atatttcatg cgcaagatta ttaatcttct tctatggcta ttctggtttt
      961 atttattatc tttttgtaga cttcgtcatt ttttattttt tcgcaattat ttaatattga
     1021 tttctccga
//
LOCUS       DACAPB010000372         1011 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.388, whole genome shotgun sequence.
ACCESSION   DACAPB010000372 DACAPB010000000
VERSION     DACAPB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.388"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..355
                     /gene="sitD"
                     /locus_tag="IT904_004753"
     CDS             <1..355
                     /gene="sitD"
                     /locus_tag="IT904_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAP3194401.1"
                     /translation="SGLHTRCLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAV
                     LLTQRFHIALLLAIGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINA
                     RTKGNTHTQDLSSPN"
     gene            complement(568..702)
                     /locus_tag="IT904_004754"
                     /pseudo
     CDS             complement(568..702)
                     /locus_tag="IT904_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(751..>1011)
                     /locus_tag="IT904_004755"
     CDS             complement(751..>1011)
                     /locus_tag="IT904_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HAP3194402.1"
                     /translation="GEELQGPTVDGNELREETRYLNVDYAAVTGLLVQVARETDDRVT
                     ALEEENTTLRQNLATADTRISTLENQVSELVALVRQLTGSEH"
BASE COUNT          240 a          268 c          232 g          271 t
ORIGIN      
        1 aagcggtttg catacgagat gtctgcatta tgggctgctg tgcatggtct ctctgaccat
       61 tgtggcgacg ctgaaagcgg taggtatcat tctctccatt tctctgctca ttgcgcctgg
      121 cgccattgcg gtactcctga cacaacgctt tcatattgcg ctcctactgg cgattggcat
      181 atcggtaata gtgtcaatga ctggtgtctg gttgtccttt tttatcgaca gtgcaccagc
      241 tccgacgatt gtcgtcttat tcgcggttgt gttcatcatg acgttcaccg ttaccagcat
      301 caacgcacgc acaaagggaa acacccatac acaggatttg tcatcaccca attagcacaa
      361 catccgagga cagcatcgtc cgctggccca cagaagtccg gaaagtcatg gaaggtcgtt
      421 gttgggagga ggtacaaatt ctctcatgca aaaaatacgt aaaatcgata atagctggaa
      481 ataattcaat acatgcactc tcgaaagtgc atcagccaac cgcagaacgt tattgcatac
      541 aacgtttctg cggcataatc ccaatgatta ctccctgaca ggggtcgtag gccactcaat
      601 atcaggtgca gttgatgtat caacacggtt cagcaacacc cgatactttt tccaggcttc
      661 cagcaacaag gtttcttcct ccgttgcgat ttccagctca acaacaatct gaacgtaccg
      721 ggaacagcct ccttcagagc ttgaaggata tcaatgttcg cttcctgtta actgccggac
      781 aagtgcaacc agttcgctta cctgattttc cagagtgctg atccgggtgt ctgctgttgc
      841 cagattttga cgtagcgttg tgttttcctc ttccagcgcg gtaacgcgat catctgtttc
      901 acgggcgacc tgaacaagta aacccgtcac ggcggcgtag tcaacattaa gatagcgcgt
      961 ttcttcgcgt agctcgttgc cgtcaacggt cgggccttgc aactcttcac c
//
LOCUS       DACAPB010000373          968 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.389, whole genome shotgun sequence.
ACCESSION   DACAPB010000373 DACAPB010000000
VERSION     DACAPB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.389"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..917
                     /locus_tag="IT904_004756"
     CDS             <1..917
                     /locus_tag="IT904_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HAP3194403.1"
                     /translation="EREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVML
                     VLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQHPALLKQATGADQLVPAPEAG
                     ATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSLDQRSQMRRIMLCVSDTIDKVV
                     KMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVATTIGEKI
                     GKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVT
                     SILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT          210 a          250 c          284 g          224 t
ORIGIN      
        1 cggagcgtga aaagaaagac ggcaagaaaa agccgccgtt ctggacgcgt attgcgctga
       61 tcctttccgc tatcggcgtg gcgttttcgc acggcgcgaa cgatggtcag aaaggcattg
      121 gtctggttat gttggtattg attggcgtcg caccagcagg cttcgtggtg aatatgaatg
      181 ccactggcta cgaaatcacc cgtacccgtg atgccatcaa caacgtcgaa gcttactttg
      241 agcagcatcc tgcgctgctg aaacaggcta ccggtgctga tcagttagta ccggctccgg
      301 aagctggcgc aacgcaaccc gcggagttcc attgccatcc gtcgaatacc attaacgcgc
      361 tcaaccgcct gaaaggcatg ttgactaccg atgtggaaag ctacgacaag ctgtcgcttg
      421 atcaacgtag ccagatgcgc cgcattatgc tgtgcgtttc tgacactatc gacaaagtgg
      481 tgaagatgcc tggcgtgagt gctgacgatc agcgcctgtt gaagaaactg aagtccgaca
      541 tgcttagcac catcgagtat gcaccggtgt ggatcatcat ggcggtcgcg ctggcgttag
      601 gtatcggtac gatgattggc tggcgtcgtg tggcaacgac tatcggtgag aaaatcggta
      661 agaaaggcat gacctacgct caggggatgt ctgcccagat gacggcggca gtgtctatcg
      721 gcctggcgag ttataccggg atgccggttt ccactactca cgtactctcc tcttctgtcg
      781 cggggacgat ggtggtagat ggcggcggct tacagcgtaa aaccgtaacc agtattctga
      841 tggcctgggt gtttaccctt ccggctgcgg tactgctttc cggcgggctg tactggctct
      901 ccttgcagtt cctgtaatcg tacacaccaa aacgagcggg tcagctggcc cgcttcagat
      961 tgttgaca
//
LOCUS       DACAPB010000374          956 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.393, whole genome shotgun sequence.
ACCESSION   DACAPB010000374 DACAPB010000000
VERSION     DACAPB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.393"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..101
                     /locus_tag="IT904_004757"
     CDS             <1..101
                     /locus_tag="IT904_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP3194404.1"
                     /translation="EQQQTPGQVENRALYPRPEGWGFTAHRIKSAI"
     gene            complement(119..787)
                     /locus_tag="IT904_004758"
     CDS             complement(119..787)
                     /locus_tag="IT904_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAP3194405.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT          260 a          215 c          210 g          271 t
ORIGIN      
        1 ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag
       61 gatggggttt tacggcgcac cggataaaaa gcgccatcta acgatggcgc gtctggaact
      121 actttttgtt aacgtcaaac atggtggcat cggtcgccat gtcatcaata acttgtttaa
      181 tattttcgaa agccattggc gtactttcgt tattcaggtc tttaccttcg ccctgacgaa
      241 cgactttgat aaccggttta ttagtcgctg catcaatcag ctcaccttca aaatagaggc
      301 gagtatccat ggtacggtgg cctgtagcca tttgcgtccc cgcaaccact aatgcaacag
      361 gaaccacttc atagaattgc agcccttctt tgctggtatc tacaccggta atggccccac
      421 ggaaaatcag actacgcggc ccagcggtgg taaccagtgg tttacgctgc gctatcgctt
      481 ctttcatttc ggtgttggta tagttcaaaa ttttatccag aactttctgc cctacctggg
      541 tcgacggttt cggtaccgga taataagtga ttgggttcca gacgatgctg tcatatttgc
      601 tttgatcaaa actcgggtct acccaacgta aaacaggttt acctgtagcc gaggttgttt
      661 cttttaaatc agagtaattg tttaaaaaac cagaatattt atctggctgg gtgattttag
      721 aggcacaacc agacagagcc aataagccgc ataaagctgc aactttcgca aatgatgtgg
      781 tacgcatgaa tagtttcctg agatgattat attactttct aagttatagc aaaaagcgcc
      841 gcttcgtgtt gtggcaaaaa aagtgttggt gaaaataaat ttttcagccc cttttcagac
      901 gacatagtca atgccgaaaa aggggctaag tcaatcatca gtagttaaat ttcagt
//
LOCUS       DACAPB010000375          955 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.394, whole genome shotgun sequence.
ACCESSION   DACAPB010000375 DACAPB010000000
VERSION     DACAPB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.394"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..347
                     /gene="tnpA"
                     /locus_tag="IT904_004759"
     CDS             <1..347
                     /gene="tnpA"
                     /locus_tag="IT904_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP3194406.1"
                     /translation="GPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVRYY
                     DKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELHIV
                     QTPDAKKSHVHF"
     gene            complement(504..>955)
                     /locus_tag="IT904_004760"
     CDS             complement(504..>955)
                     /locus_tag="IT904_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP3194407.1"
                     /translation="YCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          275 a          206 c          180 g          294 t
ORIGIN      
        1 gcggaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg atatccagtc
       61 tggtaaacag cctcaaaggc gtatcgagta ggttactgcg acgagatcga ccagatattg
      121 cagtcaggta ttacgacaaa ggcgttttgt ggagtcctgg ctattttgtc agtagctgca
      181 tcttaacgta tcaactagat ttaataacaa cgatatccgt acctaaccgg atatctaatg
      241 tcataagcat ccctttaatt atcccctcaa cctcttgtaa ctttcccact cttgagctcc
      301 acatagtcca aacgccggat gcaaaaaaat cacacgtgca tttctgaaat tgtgatggag
      361 gaagatagta tcaattatat gtgcttagaa acgtccccga ttagcgcaga agtcaccgga
      421 ttgttcagac tccaataggt tgagtatgcc gtatttattt tccaaaataa aaatcgatgc
      481 tgttgctcct tcataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt
      541 atcttaaaac gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc
      601 ctggcaatct cgctgatggg ttttactgtc gtttgtatag cagacaacgc attatttaac
      661 ctcacgtcgt ccaatatact ttgaaaactt actccctcgc ttgccagacg gcgatgcaat
      721 gtggaaacag aaatgtagag atatctcgct accttgtttg ctgtccattt tgttccaggt
      781 tcggatagca ttaggttata acaacgactt attaatgatt gtttactatc tgataaaagt
      841 aaacaattaa catgatgtgc tcctaatgaa agtagaacgc ccattgctaa gtgttcctga
      901 atttgcgttg agaagcctcg ggaaatagat gtttttagtt gctcccaaca atata
//
LOCUS       DACAPB010000376          945 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.395, whole genome shotgun sequence.
ACCESSION   DACAPB010000376 DACAPB010000000
VERSION     DACAPB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.395"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..635
                     /locus_tag="IT904_004761"
     CDS             <1..635
                     /locus_tag="IT904_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194408.1"
                     /translation="TNFTNANMFQVNFVSSTQNATISWDYLLKITPVLISISDMYSEE
                     KIKFVESCLNEPGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKEIYKSSSKIINC
                     LNRIKLPEMKEFSSEKIYDYIDIIIEDYENIKENAYLVVPQINYTMDLNIEDSSSEEF
                     LFDNGLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEYTYDYDLLNAI"
BASE COUNT          353 a          170 c          154 g          268 t
ORIGIN      
        1 caacaaattt tactaatgcc aacatgtttc aggttaattt tgttagttca acacaaaacg
       61 ccacaatctc ctgggattat ttactaaaaa taacgcctgt tttaataagc attagcgata
      121 tgtattctga agaaaaaatc aagtttgtcg aaagttgttt aaatgagcct ggagacatta
      181 ccgaagaaca attaaaaatt atgagatttg caattataaa atctatacca agggcaactc
      241 ttacagataa attagaaaat gaattaacaa aagaaatata taaaagctca tcgaaaatca
      301 tcaattgctt gaacagaatt aaattaccag agatgaaaga attctcatca gaaaaaatat
      361 atgattacat cgatataatc attgaagatt atgaaaatat caaagaaaat gcttatctgg
      421 tcgtccccca aattaattat actatggatt taaacataga agactctagc tcagaggaat
      481 tcctttttga taatggcctc gagaaagacg aaaattcttc ggacaattgc tttgaagtcg
      541 aaaaatataa cgcatatgaa gcatataact cagagaatct atattttacc agagaggaat
      601 atacgtatga ttacgacctt ttaaatgcaa tatagactta ataacttctt acaaaatcta
      661 taattaatac cgtagatatt tattcatctt accctgtgca ccaaaagcaa aaactcactg
      721 acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc ttatcaggcc
      781 tacgccatct ctgcaatata ttgaatttgc gtgcttttgt aggcaggata aggcgttcac
      841 gccgcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga acggtttatt
      901 acgtacatca ggtaaaactg accgataagc cgctttcttt tgggt
//
LOCUS       DACAPB010000377          907 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.398, whole genome shotgun sequence.
ACCESSION   DACAPB010000377 DACAPB010000000
VERSION     DACAPB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.398"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..834)
                     /locus_tag="IT904_004762"
     CDS             complement(<1..834)
                     /locus_tag="IT904_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HAP3194409.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPATIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DK"
BASE COUNT          199 a          217 c          203 g          288 t
ORIGIN      
        1 tttgtcggta agagcctgat ttactgagac agaatctttg cccctgagtc taaggatgat
       61 cgtataacgt gattttcggt ctacaagtgt ggctatatga gagttttttg tacctgagac
      121 taaatcgccc tcccaatgcc ccagagagcg tctgttatcg atatttcggg aacgttcgtg
      181 aattggtgtt ccgttcacta tgttaatcgt acctctttcg cctttgcggg tatgacgcct
      241 gccatggcga aggctatgcg accgtcgcag atgctgtata ttcaggtggt gtagcgcttc
      301 acggctacga aagtacagcg ttttataaat tgtctcaggt gatattcgca gcgttttttg
      361 acgtggtttt gttcgcctta accatcctga tatttgctct ggagaccatt tcatctccag
      421 cttttccaga acaagctttc gcaatggtaa attttgatcc agtaagcacg gttttggcct
      481 tttcgccatt ctgttggctc ggttattagc atcaacagct ttgtaatagc gtctgccccg
      541 attacgctga acttcacgtg agatcgtcga aggactgcga ttcagcgcag tagctatcgc
      601 acgaatgctc attttggctg acaaaccagc tcgtatctcc tcgcgctcag acagtgtcag
      661 gtgagctaca gcccgcttac gctcatgggg ttttatgccg ccagtatccc ttaacatagt
      721 gaagatcgtt gcgggttttg aacccaggat attcgctatt tcactgaagc ctgttccgtt
      781 cttccatagt tcaaaaacag aggctttttc ctctgctgta aatgttcgtc tcattcaaaa
      841 aacctccgca accccatgtt ttcacataac tgttgcgttg accaattgaa tctacagcgg
      901 ctttttt
//
LOCUS       DACAPB010000378          901 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.399, whole genome shotgun sequence.
ACCESSION   DACAPB010000378 DACAPB010000000
VERSION     DACAPB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.399"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..199
                     /locus_tag="IT904_004763"
     CDS             <1..199
                     /locus_tag="IT904_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194410.1"
                     /translation="EIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPT
                     ASISDLLVVVMKKATAGITIS"
     gene            267..866
                     /locus_tag="IT904_004764"
     CDS             267..866
                     /locus_tag="IT904_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP3194411.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          206 a          205 c          266 g          224 t
ORIGIN      
        1 tgaaatttat aaccgtaccg cgctggataa tacgctgatt tacagtggtg ttattgatat
       61 gcctgccggt cacggtcaca tgacactgga gttttcggtg tcagcatggc tggtaaataa
      121 ctggtatccc acagcaagta tcagcgattt gctggttgtg gtgatgaaga aagccactgc
      181 aggcatcacg attagctgaa ttttataacc cagatacggg cgccagaaat ggcgcctttt
      241 ttattgcaga aaagcgagag gtaattatgc gtaaagtttg tgcagccatt ttgtccgcag
      301 ccatctgtct ggccgtatcc ggtgcgcctg catgggcgtc tgagcagcag gccacactga
      361 gcgcagggta tcttcatgcc tcgacgaacg ttcccggtag tgatgatctg aacgggatta
      421 acgtgaaata ccgttatgag tttacggaca cgctggggct gattacgtca ttcagttatg
      481 ccaacgctga agatgagcaa aaaacgcatt acagcgatac ccgctggcat gaggattccg
      541 tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat gaatggttca
      601 gcgcgtatgc gatggcgggt gtggcttaca gccgtgtgtc gactttctcc ggggattatc
      661 tccgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccggaagt gatgacggtc
      721 gccacagcaa cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg
      781 tggccattga tattgcttat gaaggttccg gcagtggtga ctggcgcact gacggtttca
      841 tcgtgggtgt cggttataaa ttctgattag ccaggtaaca cagtgttatg acagcccgcc
      901 g
//
LOCUS       DACAPB010000379          877 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.403, whole genome shotgun sequence.
ACCESSION   DACAPB010000379 DACAPB010000000
VERSION     DACAPB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.403"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>877
                     /locus_tag="IT904_004765"
     CDS             <1..>877
                     /locus_tag="IT904_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP3194412.1"
                     /translation="EDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNK
                     YICEYLTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVERE
                     GPFYFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTA
                     EDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQ
                     TRDSVNQNQHESERNYQKAEQNSTNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGN
                     CPDCGAVMGDATYQET"
BASE COUNT          283 a          210 c          220 g          164 t
ORIGIN      
        1 gaagactgga agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct tcgctacgac
       61 cgccagacta tttttggcct tgtccgtgag cgtccgatcg acattcacaa agatcccata
      121 gcactgaaca aatatatctg cgaatacctg acgacaaagg gcgtgtttga gaatgaagaa
      181 acagacctgg gcactgttga tgttctccag tcatcagaaa cacaaactga tgcagtggaa
      241 actgaggtat ctgatatccc aaaaaatgaa accgcgccgg aagctgaacc atctgtagag
      301 cgtgaggggc cgttctattt cctcttcgca gataaggacg gagaaaaata cggtcgcgca
      361 aacaaacttt ctggtctgga taaggcactg gctgctggcg ccactgaaat cacaaaagaa
      421 gaatattttg cccgaaaaaa tggcacatac acgggcttac cgcaaaatgt agataccgct
      481 gaagattcag aacaaccaga gccgataaaa gttaccgctg acgaagtaaa caaaattatg
      541 caggcagcca atatcagcca gcctgacgcc gataagttgc ttgctgcatc acgtggtgaa
      601 tttgttgaag ggattagtga cccgaatgat ccgaaatggg ttaaggggat ccagacccgc
      661 gattctgtga accagaacca gcatgaatcg gaacggaact accaaaaagc ggaacaaaac
      721 agcacaaatg cgttacaaaa cgagccagaa acgaaacagc ctgaaccagt ggcgcaacag
      781 gaagtggaaa aagtctgcac cgcctgcggt cagaccggcg gcggcaactg ccctgattgt
      841 ggcgcggtga tgggcgacgc aacatatcaa gaaacat
//
LOCUS       DACAPB010000380          851 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.405, whole genome shotgun sequence.
ACCESSION   DACAPB010000380 DACAPB010000000
VERSION     DACAPB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.405"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..345
                     /locus_tag="IT904_004766"
                     /pseudo
     CDS             1..345
                     /locus_tag="IT904_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            379..>851
                     /locus_tag="IT904_004767"
     CDS             379..>851
                     /locus_tag="IT904_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAP3194413.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRDSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERR"
BASE COUNT          234 a          203 c          214 g          200 t
ORIGIN      
        1 cacattgatc ggctgaacag tattgaatca cgggcattgc cggaaggtat tgatcgtgcc
       61 attcatcaaa acaggttact gaaacttgcg cgagaagggc gaaagatgag cagtagggac
      121 ctgactcgat tttcagcagc ccgacgtcat gcaattctgg tgtgtgtcct ggaagatgcc
      181 agagctacgc tgacagatga aattattgag ttgcatgaac gaatattgaa cagtctgttc
      241 aacaaggcta aaaaaacaca ggctgaacgt cttcaacaaa caggaaggtt aatccagtta
      301 aaattaaagc agtatattga tatcggacag gctttatctg aggcccggct ttgttgaata
      361 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ctcccgacaa cacagaccat
      421 tccgtggcaa agcaaaagtt cagaatcacc aactggtcca cctacaacaa agctctcatc
      481 aaccgtgact ccctcacttt ctggctggat gatgaggcga ttcaggcctg gtatgagtcg
      541 gcaacgcctt catcacgagg aaggccccag cgctattctg atctcgccat caccaccgtt
      601 ctggtgatta aacgcgtatt ccggctgacc ctgcgggctg cgcagggttt tattgattcc
      661 atttttgccc tgatgaacgt tccgttgcgc tgcccggatt acaccagtgt cagtaagcgg
      721 gcaaagtcgg ttaatgtcag tttcaaaacg tccacccggg gtgaaatcgc acacctggtg
      781 attgattcca ccgggctgaa ggtctttggt gaaggcgaat ggaaagtcag aaagcacggc
      841 aaagagcgcc g
//
LOCUS       DACAPB010000381          784 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.406, whole genome shotgun sequence.
ACCESSION   DACAPB010000381 DACAPB010000000
VERSION     DACAPB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.406"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>784
                     /locus_tag="IT904_004768"
     CDS             <1..>784
                     /locus_tag="IT904_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAP3194414.1"
                     /translation="DQRHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNF
                     DIDELEALHQELEARIASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQ
                     NSLNQLSEQCGEEFTSSQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQP
                     GGSEDQRLNALAERFGGVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHL
                     EGLTDCPEDLYLIEGDPQSFDDSVFSVDELEKAVVVKIADRQ"
BASE COUNT          189 a          194 c          240 g          161 t
ORIGIN      
        1 tcgatcagcg tcacctggca gagcaggttc agccgctgcg gatgcgatta agcgaactgg
       61 aacagcgtct acgcgagcag caagaagctg agcgtctgct ggcagatttc tgcaaacgtc
      121 agggcaagaa ttttgatatc gacgaactgg aagccctgca tcaggaactg gaagcacgca
      181 ttgcctctct ttccgatagc gtgtctaacg cccgtgaaga gcgcatggca ctgcgccagg
      241 agcaggaaca gctgcagtct cgcattcaga gtttgatgca gcgtgcgccg gtttggctgg
      301 cagcgcaaaa cagtctcaac cagttgagcg aacagtgcgg cgaagagttt acctccagcc
      361 aggacgtcac cgaatatctg caacagttgc tggagcgtga gcgagaggcg attgttgaac
      421 gcgatgaagt gggcgcgcgc aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc
      481 ctggcggctc tgaagatcag cgtctgaacg cgctggcgga gcgttttggt ggtgtgctgc
      541 tgtcagaaat ttatgacgac gttagcctag aagatgcgcc gtacttctca gcgctgtatg
      601 gcccgtcacg ccatgccatc gtggtgccag atctgtcaca ggtaactgaa cacctggaag
      661 gcttgaccga ttgcccggaa gatctctatc tgatcgaagg agatccgcag tcattcgatg
      721 acagcgtgtt cagcgttgat gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc
      781 agtg
//
LOCUS       DACAPB010000382          751 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.408, whole genome shotgun sequence.
ACCESSION   DACAPB010000382 DACAPB010000000
VERSION     DACAPB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.408"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>751
                     /locus_tag="IT904_004769"
     CDS             <1..>751
                     /locus_tag="IT904_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP3194415.1"
                     /translation="AFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDV
                     IEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQ
                     SVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPE
                     KEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSF
                     MLRLTVAADDGSERLVSTARTTETTYRFTQLA"
BASE COUNT          155 a          206 c          261 g          129 t
ORIGIN      
        1 atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa
       61 cagaactgct ggaaacgcag accgtggatt tcagcgtggg tgccgaaggg cttcgccatg
      121 tgccgggtga tgttattgaa atctgcgatg atgactatgc ggggatcagc accggcgggc
      181 gcgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc
      241 catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg
      301 aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg
      361 ctgaatacag cgtatggggg ctgaagctgc cgacgctgcg ccagcgactg ttccgctgcg
      421 tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg
      481 aaaaagaggc catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga
      541 atggtgtcac gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg
      601 aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg agctttatgc
      661 ttcgcctgac cgtggcagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga
      721 cggaaaccac ttaccgcttc acacaactgg c
//
LOCUS       DACAPB010000383          749 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.409, whole genome shotgun sequence.
ACCESSION   DACAPB010000383 DACAPB010000000
VERSION     DACAPB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.409"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(69..>749)
                     /locus_tag="IT904_004770"
     CDS             complement(69..>749)
                     /locus_tag="IT904_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP3194416.1"
                     /translation="SNELTEAAYYLSLKAKRVLWLCLMQTYFTASVSEDDDEMAVLGD
                     STFKVKVADYQQIFQVSRNQAIKDVKEGVFELSRSAVIFYPKEGSFDCVARPWLTEAG
                     SRSARGIWEIEFNHKLLRYIYGLTNQFTTYSLRDCGSLRNPRTIRLYESLAQFKSSGL
                     WVTTHAWLNDRFLLPESQQKNLAELKRSFLDPALKQINEKTPLLAKYSIDDSGKFLFS
                     IIDKQNPV"
BASE COUNT          211 a          162 c          170 g          206 t
ORIGIN      
        1 gtgtggaaat tcgcccatct tcatgacaaa aatttgtcaa atgacaggct catgtgtgct
       61 gatttatgtc agacgggatt ttgcttatca attattgaga acagaaattt tcctgaatca
      121 tcaatactat acttagcaag taaaggtgtt ttctcattta tctgcttgag agcaggatca
      181 aggaaagatc gtttcaactc tgccaagttc ttctgttggg attccggcaa aaggaaacgg
      241 tcatttaacc aagcatgagt agtaacccat aagcctgaag atttgaattg agcaagactt
      301 tcataaaggc ggatcgtccg tggatttcga agactgccac aatcgcggag cgagtaggtg
      361 gtgaactggt tcgtcaggcc gtaaatgtac cgcaggagtt tatggttaaa ttcgatttcc
      421 cagataccac gagctgatcg gctgccagcc tctgttagcc aggggcgcgc gacgcagtca
      481 aaactccctt ctttcgggta aaagattacc gcagaacggc ttaactcaaa cacgccttct
      541 ttaacatcct tgatagcctg gttacggctt acctgaaaaa tttgctgata gtcagccacc
      601 tttactttga aagtagagtc accgagtaca gccatctcat catcatcttc gcttactgaa
      661 gctgtgaaat acgtctgcat aagacataac cagagaacgc gctttgcttt tagcgagagg
      721 tagtaagcag cttcggtaag ctcgttaga
//
LOCUS       DACAPB010000384          733 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.410, whole genome shotgun sequence.
ACCESSION   DACAPB010000384 DACAPB010000000
VERSION     DACAPB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.410"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..544
                     /locus_tag="IT904_004771"
     CDS             <1..544
                     /locus_tag="IT904_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAP3194417.1"
                     /translation="NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWV
                     LATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLI
                     ALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLL
                     VAATLLAQNLFTHGYALGKL"
BASE COUNT          206 a          183 c          157 g          187 t
ORIGIN      
        1 caatccaatc tcatgccaaa ttctattgta taaatctcgc tctaaaggcc gaaaaaatca
       61 gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa atctactcag cgtcggcaaa
      121 ggagccatgg gttctagcaa ctaacttacc tgttgaaatt cgaacaccca aacaacttgt
      181 taatatctat tcgaagcgaa tgcagattga agaaaccttc cgagacttga aaagtcctgc
      241 ctacggacta ggcctacgcc atagccgaac gagcagctca gagcgttttg atatcatgct
      301 gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg ctcagaaaca
      361 aggttgggac aagcacttcc aggctaacac agtcagaaat cgaaacgtac tctcaacagt
      421 tcgcttaggc atggaagttt tgcggcattc tggctacaca ataacaaggg aagacttact
      481 cgtggctgca accctactag ctcaaaattt attcacacat ggttacgctt tggggaaatt
      541 atgaggggat ctctcagcgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
      601 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
      661 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
      721 ggcgcaaagt gcg
//
LOCUS       DACAPB010000385          733 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.412, whole genome shotgun sequence.
ACCESSION   DACAPB010000385 DACAPB010000000
VERSION     DACAPB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.412"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            539..>733
                     /locus_tag="IT904_004772"
     CDS             539..>733
                     /locus_tag="IT904_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194418.1"
                     /translation="MLAQTTLSTELYIPDGFVKQTAAPSGYIEGNVVRIYDQVNKPTK
                     ADLGLSNAMLTGAFGLGGSGI"
BASE COUNT          206 a          175 c          188 g          164 t
ORIGIN      
        1 gaatgcggat aacagcgcac aggcggcagc agactcacaa actgcatcgg caaattccgc
       61 gacagcagcc aaaaaatcag aaaccaacgc gaaaaatagt gagtcagcag caaaggtcag
      121 cgaaaccaac gctaaagcgt cagagaacaa ggcgaaagaa tatctcgaca aggtcgggag
      181 actcgtcagc ccgatgacgc aatacgattg gcctgttgtt actgctagtg agtctcttta
      241 catcaagatc gcgaaacttt ccgatcctgg aaccagcaga agtcatgtaa cgctaatggt
      301 tactaacgct ggtaactacg gctcccctta cggaaacatt gactttatcg agatctcggc
      361 gcgcggtctg ccttcttcgc ttactgcgga taatgtttct cgtcatctga gtatacgccg
      421 cttagggtca accgggctga ccgataacaa ccagatgcgt tacggcctgg ttaaaggtga
      481 cggctttatt gaggtttggg cattccaggg tgcgtttatt aacgacgcaa aggttgcggt
      541 gctggcgcag acaacactaa gcacagaatt atacatccca gacggatttg ttaagcaaac
      601 cgccgcgcct tctggatata ttgaaggcaa cgttgtaagg atttacgacc aggtaaacaa
      661 gccgactaaa gcagatttgg gtctttctaa tgctatgctt acaggcgctt tcggtcttgg
      721 cggtagcggg ata
//
LOCUS       DACAPB010000386          730 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.413, whole genome shotgun sequence.
ACCESSION   DACAPB010000386 DACAPB010000000
VERSION     DACAPB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.413"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..293
                     /gene="tsf"
                     /locus_tag="IT904_004773"
     CDS             <1..293
                     /gene="tsf"
                     /locus_tag="IT904_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HAP3194419.1"
                     /translation="AEVVEKEYQVQLDIAMQSGKPKEIAEKMVEGRMKKFTGEVSLTG
                     QPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEVAAMSKQS"
     gene            440..>730
                     /locus_tag="IT904_004774"
     CDS             440..>730
                     /locus_tag="IT904_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HAP3194420.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHR"
BASE COUNT          193 a          154 c          215 g          168 t
ORIGIN      
        1 ccgctgaagt ggtagaaaaa gaataccagg tacagctgga tatcgcgatg cagtctggta
       61 agccgaaaga aatcgcagag aaaatggttg aaggccgcat gaagaaattc accggcgaag
      121 tttctctgac tggtcagccg ttcgttatgg aaccaagcaa aaccgttggt cagctgctga
      181 aagagcataa cgctgaagtg actggcttca tccgcttcga agtgggtgaa ggcatcgaga
      241 aagttgagac tgactttgca gcagaagttg ctgcgatgtc caagcagtct taattatcga
      301 aaaggagccg cctgagggcg gcttcttttt gcgcccatct tgtaaattca gctaaccctt
      361 atggggctgc gctgaaaagc gacgtaaaat gtcgccggta ttaattcatt tcaatcgttg
      421 acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac gcattctgct
      481 taagttgagt ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg caagcatact
      541 ggatcgcatg gctcaggaaa tcaaagaact ggttgaactg ggtattcagg ttggtgtggt
      601 gattggtggg ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt
      661 tgtgggcgac cacatgggga tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc
      721 actgcaccgc
//
LOCUS       DACAPB010000387          725 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.415, whole genome shotgun sequence.
ACCESSION   DACAPB010000387 DACAPB010000000
VERSION     DACAPB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.415"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..150
                     /locus_tag="IT904_004775"
     CDS             <1..150
                     /locus_tag="IT904_004775"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP3194421.1"
                     /translation="VKARPARITESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKM
                     IGDLL"
     gene            265..>725
                     /gene="mog"
                     /locus_tag="IT904_004776"
     CDS             265..>725
                     /gene="mog"
                     /locus_tag="IT904_004776"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAP3194422.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVV"
BASE COUNT          172 a          178 c          202 g          173 t
ORIGIN      
        1 gtgaaagcgc gtccggcgcg tatcactgag tccgagttcc tgtggcagca caaccaggat
       61 ccaatggcag tagataaact ggcggaaggt atccgtaagt ttgctattga ccaggaaaaa
      121 ctggaaaaaa tgatcggcga tctgctgtaa tcattcttag cgtgaccggg aagtcggtca
      181 cgctacctct tctgaagcct gtctgtcact cccttcgcag tgtatcattc tgtttaacga
      241 gactgtttaa acggaaaaat cttgatgaat actttacgta ttggcttagt ttccatctct
      301 gatcgcgcat ccagcggcgt ttatcaggat aaaggtatcc ctgcactgga agaatggctg
      361 acatcggcgc taaccacgcc gtttgaactg gaaacccgct taatccccga tgagcaggcg
      421 atcatcgagc aaacgttgtg tgagctggtg gatgaaatga gttgccatct ggtgctcacc
      481 acgggcggaa ctggcccggc gcgtcgtgac gtaacgcccg atgcgacgct ggcagtagcg
      541 gaccgcgaga tgcctggctt tggtgaacag atgcgccaga tcagcctgca ttttgtacca
      601 actgctatcc tttcgcgtca ggtgggcgtg attcgcaaac aggcgctgat ccttaactta
      661 cccggtcagc cgaagtctat taaagagacg ctggaaggtg tgaaggacgc tgcgggtaac
      721 gttgt
//
LOCUS       DACAPB010000388          721 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.416, whole genome shotgun sequence.
ACCESSION   DACAPB010000388 DACAPB010000000
VERSION     DACAPB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.416"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..616
                     /locus_tag="IT904_004777"
     CDS             <1..616
                     /locus_tag="IT904_004777"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAP3194423.1"
                     /translation="KLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFR
                     EEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADGRK
                     VKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKD
                     AVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
BASE COUNT          184 a          164 c          204 g          169 t
ORIGIN      
        1 gaaactggtt atcgaaaact atcagggcga aggcgaaatg gttaccatgc cgaaccatcc
       61 gaacaaaccg gaaatgggca gccgtcaggt gccgtttagc ggtgagattt ggattgatcg
      121 cgccgatttc cgcgaagaag ctaacaagca gtacaaacgt ctggtgctgg gtaaagaagt
      181 gcgtctgcgt aatgcttatg tgattaaggc agaacgcgtc gagaaagatg ccgaaggtaa
      241 tatcaccacc atcttctgta cttatgacgc cgatacctta agcaaagatc cggcagatgg
      301 tcgtaaagtc aaaggtgtta ttcactgggt gagcgcggca catgcgctgc cggttgaaat
      361 ccgtttgtat gaccgtctgt tcagcgtgcc taacccaggt gctgcggatg atttcctgtc
      421 ggtgattaac ccggaatcgc tggtgatcaa acagggcttt gctgaaccgt cgctgaaaga
      481 tgcggttgcg ggtaaagcat tccagtttga gcgtgaaggt tacttctgcc tcgatagccg
      541 ccattctacg gcggaaaaac cggtatttaa ccgcaccgtt gggctgcgtg atacctgggc
      601 gaaagtaggc gagtaatttt aagtttcgct atgccggatg gggcgtttac gtcgcatccg
      661 gcaaggaaca gacaaacagt ttcaaacgct aaattgcctg atgcgctacg cttatcaggc
      721 c
//
LOCUS       DACAPB010000389          714 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.418, whole genome shotgun sequence.
ACCESSION   DACAPB010000389 DACAPB010000000
VERSION     DACAPB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.418"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            118..558
                     /locus_tag="IT904_004778"
     CDS             118..558
                     /locus_tag="IT904_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP3194424.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDAL
                     RILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGV
                     IFEGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
BASE COUNT          226 a          142 c          170 g          176 t
ORIGIN      
        1 tataaagata cagaaaggag aataaaaaca ttaacaaaat ctaagggtta cagttgacat
       61 agttatcaat aatgataaca ctgttatcat tattgataac tcaaataggg ttcaaagatg
      121 agtaaggatg tcgagagaat cactcagata aatgaccagg gtgagattat cggcggcttc
      181 gttgccgtta tccgccctaa acaaaaatca gcatttcaga ggcatttcac catgaatcaa
      241 gacgcattac ggatcttggc taaggaatta accggggagc aattcaaggt cttaatgctg
      301 atgcttgcag atctggatta cgaaaacttc attcagatcg cccaagcaga tattgctgaa
      361 actttaggga tgcagaaaac gaatgttagt cgcgccgttc gcgcgctact ggatgttggg
      421 gtgatctttg agggacctaa agttggcaga tcaaaaacat accggctgaa tgaacaattt
      481 ggttggaaag gcaccgtaac caaccacaaa aaggcactca aaaacggctt tagtgtcatt
      541 cagggcggcc ggatttaaag agccagcggc aggcggacaa tcagggctac gtgttaacgt
      601 tttgaccatg attgtctatc ctgcattgct cttttgccgc atgtcagttc gatgcgatgc
      661 aggatagggc aaacgccgca aaatgacgtc attgacgtca ttcgcaacct ttgc
//
LOCUS       DACAPB010000390          712 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.419, whole genome shotgun sequence.
ACCESSION   DACAPB010000390 DACAPB010000000
VERSION     DACAPB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.419"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..691
                     /locus_tag="IT904_004779"
     CDS             <1..691
                     /locus_tag="IT904_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194425.1"
                     /translation="ADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVA
                     EHGDYQATEIAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVH
                     AAIIADQMMSNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPA
                     DRDDLCLDPRRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIA
                     AAIKQVRQTSY"
BASE COUNT          148 a          193 c          213 g          158 t
ORIGIN      
        1 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
       61 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
      121 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
      181 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
      241 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
      301 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
      361 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
      421 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
      481 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
      541 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
      601 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
      661 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct at
//
LOCUS       DACAPB010000391          710 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.420, whole genome shotgun sequence.
ACCESSION   DACAPB010000391 DACAPB010000000
VERSION     DACAPB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.420"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            278..505
                     /locus_tag="IT904_004780"
     CDS             278..505
                     /locus_tag="IT904_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAP3194426.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKE
                     SDSGLLGYTDANGVKQQINKTDVKEVSEIPH"
BASE COUNT          223 a          188 c          135 g          164 t
ORIGIN      
        1 attacgcgcg gaaaaaaagg atctcaagaa gatcctttat aaattagaca tccaacctca
       61 caaagaggaa tacttaaatc atcttggtat atgtatcggt cggattacat cagatataag
      121 cccgcactgc cccctccccg ccagacacca cactttaacc ataacacctc gcacctaact
      181 cgatatgcag cccaccatca agcctctttt cacataaaaa atgacctata gttagatggt
      241 tagtgaccaa aactgatatg gataaaagga gctggctttg aaaaagattt ttgtttcagt
      301 gttcgcagcg gcagttgcgc tttcagccct caccggctgc acgcgcacaa gctatgccat
      361 acacaccaat gacggtcgca ctattgtcag cgacggcaaa cctaaagaat cagactcagg
      421 cctgcttggc tatacagacg cgaatggtgt caaacagcag atcaataaaa ccgacgttaa
      481 agaagtctcc gaaattccgc actgagcatc ttctccgaaa aaggccgcta atgtggcctt
      541 tttctttacc tgtggttctc cgccaaaatc ccagcaaatt gcatcaccaa agctaaaagc
      601 tttcagggtt gtctaaaaag cacaagacat aagaggaagt gcggtatttt ataatcaagc
      661 ccccaagaat ttaccataac atccgttgcc cgcaccgcct gagacgcgtt
//
LOCUS       DACAPB010000392          707 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.421, whole genome shotgun sequence.
ACCESSION   DACAPB010000392 DACAPB010000000
VERSION     DACAPB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.421"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..>707)
                     /locus_tag="IT904_004781"
     CDS             complement(<1..>707)
                     /locus_tag="IT904_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP3194427.1"
                     /translation="HVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGE
                     YQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTV
                     RAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSE
                     KRIADIRQVETTARYLGTALYWIAASINIKPGHNYYFYVRSVNTVGKSAFVEAVGQPS
                     DDASGYLDFFKGEIGKT"
BASE COUNT          139 a          226 c          183 g          159 t
ORIGIN      
        1 ggttttccct atctcgcctt tgaaaaaatc cagatagccg gatgcgtcat cactcggctg
       61 accaacagcc tccacgaatg ccgatttgcc aacggtgttc acactgcgaa cgtaaaaata
      121 ataattatgg cccggtttga tattgatact ggcggctatc cagtacaacg ccgtaccaag
      181 atagcgtgct gtggtttcaa cctgcctgat atccgcaatc cgcttttccg agaaccagaa
      241 ctcaaactgt accgtcggat cataaaccgc aagatgcggc gtggcggtta tctgaaaata
      301 gcccggcgtc agctcaatcc gcgacggcgc tgccggtgcg gcaatccgga acgataccga
      361 cgccggatcg ccctgctgcc cccacgcatt tactgcccgg actgtcagcc tgtagttccc
      421 cagagccagt tgtgtgaagc ggtaagtggt ttccgtcgtc cgggccgtgc tgaccagccg
      481 ctcactgccg tcatccgctg ccacggtcag gcgaagcagg aagctcacgc ccttcaccac
      541 cttcggcgtg tcccagcggg ccagcacctg gtattccccg ctgtctgcgg tgacttctgc
      601 ggtcagatgc tgcactgctg gcggcgtgac accgtttacc gttccgctct ggtcgccgtc
      661 aaagtgcgcc ccgttatcca cgatggcttc tttctccggt acatgct
//
LOCUS       DACAPB010000393          681 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.424, whole genome shotgun sequence.
ACCESSION   DACAPB010000393 DACAPB010000000
VERSION     DACAPB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.424"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(103..>681)
                     /gene="aph(3'')"
                     /locus_tag="IT904_004782"
     CDS             complement(103..>681)
                     /gene="aph(3'')"
                     /locus_tag="IT904_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP3194428.1"
                     /translation="EGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSVDQ
                     CPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQERTD
                     MVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAE
                     RAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          170 a          196 c          186 g          129 t
ORIGIN      
        1 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
       61 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
      121 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
      181 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
      241 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
      301 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
      361 atggcaaaca accatatcgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac
      421 acgagccaaa agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt
      481 gacggcattg cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc
      541 aaacggacat tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg
      601 ccacgctttg agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt
      661 tatcaccaag catgcaccct c
//
LOCUS       DACAPB010000394          670 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.426, whole genome shotgun sequence.
ACCESSION   DACAPB010000394 DACAPB010000000
VERSION     DACAPB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.426"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..>670)
                     /gene="yneK"
                     /locus_tag="IT904_004783"
     CDS             complement(<1..>670)
                     /gene="yneK"
                     /locus_tag="IT904_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAP3194429.1"
                     /translation="CDEEWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKV
                     QFKLTYPVKNGRHKENIEFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQT
                     GVERVLFLFLNDFIEQFPMINLGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNF
                     ISRGNFVILPRLWSNSEVHRWFVNKDPNLITAILDITDGELKEDLLQSLMDSLGSNKH
                     VLPEV"
BASE COUNT          199 a          120 c          147 g          204 t
ORIGIN      
        1 acttcgggta gtacatgttt gttagaacct aatgaatcca tcaggctttg caataaatcc
       61 tcttttaatt cgccgtctgt tatgtcgaga atggcggtga tcaagttagg atctttattg
      121 acgaaccatc tgtgaacctc agagttactc cataatcggg ggagtatgac aaaattaccg
      181 cgagagataa aattcaggac aagcaaatca aactggcgta agtaatctgc agcggtatgg
      241 tgatccggag gcaggggttc aatatgtggt gtatgcgctc ttttgatggg aacgccaagg
      301 ttgatcattg gaaattgttc aataaaatca ttcagaaaca gaaagagcac acgctcgacg
      361 ccggtctgaa atacgcgccc catagtgaca ggctttgggt tgggagcgca aaaaatatta
      421 atttctccat catggcgaaa attatctaaa taaatagggc ttaaatttat tactacctga
      481 aattcaatat tttctttgtg tcttccattc ttgacgggat aagtgagttt aaactgaacc
      541 ttttcattgt atagtgtgtc gttaccccga aaagaagtcc tctgaatata tgataagttg
      601 tcaaggttat taaatatctt ttttgtggct gagttgatgt ttaaagacgt gcgccactct
      661 tcatcacata
//
LOCUS       DACAPB010000395          657 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.427, whole genome shotgun sequence.
ACCESSION   DACAPB010000395 DACAPB010000000
VERSION     DACAPB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.427"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            299..>657
                     /gene="sitA"
                     /locus_tag="IT904_004784"
     CDS             299..>657
                     /gene="sitA"
                     /locus_tag="IT904_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAP3194430.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEG"
BASE COUNT          179 a          155 c          127 g          196 t
ORIGIN      
        1 tcccttcatt tagtgactgc ccctgggttt ttcatgctct cctgcgtcct agagaacttg
       61 ccaataacct acattatttt tgctttgcca ttaccaatcg tcctgaacaa gttcatttta
      121 aagccaaagc gctgcccaac ttttatcctc aataccacat cgtcagcagg aaataaccta
      181 atccctgtgt tgggatctta taatcgctaa tgataattat ttacatttta tagcttctgc
      241 tataaaacat agtagttgct atgtttattc taattttgct cactataggt actaaatcat
      301 gctctcaata aaaaaagtaa ccatgctctt ggggtgccta gtcctcacct gctcgatagc
      361 atttcaggcg agtgcagctg aaaaattcaa ggtcattaca acttttacca tcatcgcaga
      421 tatggccaaa aacgtggctg gagatgctgc agaagtctca tccataacca agcctggtgc
      481 agaaattcat gagtatcagc ctacccctgg cgatattaaa cgtgcgcagg gggcacaact
      541 gattctcgcc aatggtatga atctggaatt gtggttccaa cgcttttacc agcatctcaa
      601 tggggttcca gaagtaattg tctcttcggg tgtgacgcca gtagggatca ccgaagg
//
LOCUS       DACAPB010000396          646 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.430, whole genome shotgun sequence.
ACCESSION   DACAPB010000396 DACAPB010000000
VERSION     DACAPB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.430"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>646
                     /locus_tag="IT904_004785"
     CDS             <1..>646
                     /locus_tag="IT904_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HAP3194431.1"
                     /translation="QYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRP
                     EALRRFELMVEEVARNASAVAQNTAAAKKSASDARTSAREAATHATDAADSARAASTS
                     AGQAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQK
                     SAATSASTATTKASEAATSARDASASKVAAKSSETSAASSAGSAASSATAAGNSA"
BASE COUNT          157 a          198 c          192 g           99 t
ORIGIN      
        1 cagtacagcg ttattctgtt ggtggaaggc ttcccgccat cgcatgccgg gaccatcacc
       61 gtgtatgaag actcacaacc gggtacgctg aatgattttc tcggtgccat gacggaggat
      121 gatgtccgtc cggaggcact gcgccgcttt gaactgatgg tggaagaggt ggcgcgtaac
      181 gcgtccgcag tggcacagaa cacggcagcc gcgaagaagt cagccagtga tgcccgcaca
      241 tcagcccgtg aggcggcaac ccatgcgact gatgctgcgg actccgcacg cgcagccagc
      301 acgtcagccg gacaggccgc gtcgtcggct cagtcagcgt cttccagcgc aggaacggca
      361 tcaacaaagg ctactgaagc atcaaaaagt gctgccgctg cagagtcttc aaaaagcgcg
      421 gcagccacca gtgccggtgc agcgaaaacg tcagaaacga atgccgcagc atcacaaaaa
      481 tctgcggcca cttctgcatc caccgcgacc acgaaagcgt cagaagctgc cacctcagcc
      541 cgggatgcgt cggcttcaaa agtggcggca aaatcatcag aaacgagcgc agcctcgagc
      601 gccggcagtg cagcttcctc ggcaacggcg gcaggaaatt ccgcga
//
LOCUS       DACAPB010000397          598 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.434, whole genome shotgun sequence.
ACCESSION   DACAPB010000397 DACAPB010000000
VERSION     DACAPB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.434"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..138
                     /locus_tag="IT904_004786"
     CDS             <1..138
                     /locus_tag="IT904_004786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAP3194432.1"
                     /translation="NQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRL
                     K"
     gene            135..>598
                     /locus_tag="IT904_004787"
     CDS             135..>598
                     /locus_tag="IT904_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP3194433.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDL"
BASE COUNT          143 a          162 c          175 g          118 t
ORIGIN      
        1 aatcagcaga cgtcttctga acgtgaactg gagatgtcta ccgagattgc acgtctcaaa
       61 cgccagctgg cagaacggga tgaagagctg gctatcctcc aaaaggccgc gacatacttc
      121 gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca
      181 aagcaatgtg ccgcgtgctc cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc
      241 ggacaaggat aagcacgcgt cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg
      301 cttttacccg gtcaaaacag cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc
      361 agggttaccc ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg
      421 caaaggcctc ccggaagttc agcccggtca gctaccgcgc acacggcctg cctgtgtcag
      481 aaaatctgtt ggagcaggat ttttacgcca gtggcccgaa ccagaagtgg gcaggagaca
      541 tcacgtactt acgtacagat gaaggctggc tgtatctggc agtggtcatt gacctgtg
//
LOCUS       DACAPB010000398          582 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.438, whole genome shotgun sequence.
ACCESSION   DACAPB010000398 DACAPB010000000
VERSION     DACAPB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.438"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            188..>582
                     /locus_tag="IT904_004788"
     CDS             188..>582
                     /locus_tag="IT904_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HAP3194434.1"
                     /translation="MNGAKVAVLAQTARTELYIPDGFVKQTAAPSGYVESPVVRIYDQ
                     LNKPTKADLGLSNAMLTGAFGLGGSGISTNGKMSDVEILKALRDKGGHFWRGDKPTGS
                     TATIYSHGSGIFSRCGDTWSAINIDYSTA"
BASE COUNT          142 a          135 c          157 g          148 t
ORIGIN      
        1 cggaaacatt gactttatcg agatctcggc gcgcggtctg ccttcttcgc ttactgctga
       61 taatgtatct cgttacctga gtatacgccg tttagggcca accgggctaa tcaatagcat
      121 gcaaatgcgt tacggcctgg ttaaagatga tggctttatt gaggtttggg ccttccagcg
      181 tgcatttatc aacggcgcaa aggttgcggt actggcgcag acggcacgca cggaattata
      241 cattccagac ggatttgtta agcaaaccgc cgcgccttct ggatatgttg aaagccccgt
      301 tgtaaggatt tacgaccagt taaacaagcc gactaaagca gatttgggtc tttctaatgc
      361 tatgcttaca ggcgctttcg gtcttggcgg tagcgggata tcaacaaacg gcaagatgag
      421 cgatgtagag atcttaaaag ctctgcgtga caaaggtggt catttctggc gcggtgataa
      481 gccgaccgga agcacggcga ccatttatag ccacggttct ggtatattct cgcggtgcgg
      541 cgatacgtgg tcagcgatca atatcgacta ctcaaccgcg aa
//
LOCUS       DACAPB010000399          581 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.439, whole genome shotgun sequence.
ACCESSION   DACAPB010000399 DACAPB010000000
VERSION     DACAPB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.439"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            350..>581
                     /locus_tag="IT904_004789"
     CDS             350..>581
                     /locus_tag="IT904_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP3194435.1"
                     /translation="MTKKTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKTGCTP
                     ETLRVWVRQYERDTGGGDGGLTTAERQRLKELE"
BASE COUNT          162 a          139 c          150 g          130 t
ORIGIN      
        1 ctatctgtca ataaaaagac tccagcaaat gtaaacgttt tacatcgctt aagcctatgg
       61 aatttacata aaaaaaaagg gcgtgccgag tctttcgacc caatcattat ctcggtaact
      121 ccattcacaa gagcaggaaa taaccccttc aacaaataac agactgatgc ctgcgagcgc
      181 gctccccagg tttccgggct gaaagtaccg ccgcactcgt ttaacgcgga acagtctgtg
      241 ttggcggttt aatggtggat gttgctgtag accacaactg atgaggcaac ggagttgaac
      301 cgccccggaa atcctggaga ctaaacttcc tgagaaagag gtaaacagga tgactaaaaa
      361 aacacgtttt tcccccgaag tccgtcaacg ggcagttcgt atggttctgg aaagtcaggg
      421 cgaatatgac tcacaatggg cggcaatttg ttccattgct ccaaagactg gctgtacgcc
      481 ggagactctg cgtgtctggg ttcgccagta tgagcgggat accggaggcg gtgatggtgg
      541 gcttaccacc gctgaacgtc agcgtctgaa agagctggaa c
//
LOCUS       DACAPB010000400          561 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.444, whole genome shotgun sequence.
ACCESSION   DACAPB010000400 DACAPB010000000
VERSION     DACAPB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.444"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(4..477)
                     /locus_tag="IT904_004790"
     CDS             complement(4..477)
                     /locus_tag="IT904_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAP3194436.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          142 a          139 c          121 g          159 t
ORIGIN      
        1 ttgttaaccc tttttccaaa tttgatagca atagttaatg tttgaagtaa agtgttgctc
       61 aaaaacaact tcgaaggtat ttggaatact cgggaagaaa acatccccct ctggctcgat
      121 gtcgatcgtc gataagtgga gcgtagaggc catgggtaat gtttctcggt aaatttctcc
      181 gccaccagac actataacgt gaccggtgaa ttcagctagc ctgtccatgg cctcttcgat
      241 tgactgaaat acaactacat tgtcatcatt tgatgtccaa cctgagcggg taacgaccgc
      301 gtatttccta ttggggagtg cgcccataga ttcaaacgtc ttgcgaccca ccagaagcca
      361 ctgattgtag gtcaatgctt taaaaagtag ctgctcccct ttcgcggacc agggtatgtc
      421 tggaccgcaa ccaatcacgc cgtttttcgc tttcgcagcc atcaatgata ctttcaaggt
      481 tctcatcctg ggttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
      541 tccataacat caaacatcga c
//
LOCUS       DACAPB010000401          550 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.447, whole genome shotgun sequence.
ACCESSION   DACAPB010000401 DACAPB010000000
VERSION     DACAPB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.447"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..>550)
                     /gene="intI1"
                     /locus_tag="IT904_004791"
     CDS             complement(<1..>550)
                     /gene="intI1"
                     /locus_tag="IT904_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAP3194437.1"
                     /translation="VHWVRAFIRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQ
                     ALAALLFFYGKVLCTDLPWLQEIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQL
                     LYGTGMRISEGLQLRVKDLDFDHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARA
                     WWLKDQAEGRSGVALPDALERKY"
BASE COUNT          120 a          188 c          155 g           87 t
ORIGIN      
        1 gatacttccg ctcaagggcg tcgggaagcg caacgccgct gcggccctcg gcctggtcct
       61 tcagccacca tgcccgtgca cgcgacagct gctcgcgcag gctgggtgcc aagctctcgg
      121 gtaacatcaa ggcccgatcc ttggagccct tgccctcccg cacgatgatc gtgccgtgat
      181 cgaaatccag atccttgacc cgcagttgca aaccctcact gatccgcatg cccgttccat
      241 acagaagctg ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg cgaaccactt
      301 catccggggt cagcaccacc ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa
      361 gccagggcag atccgtgcac agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct
      421 gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga
      481 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
      541 cccagtggac
//
LOCUS       DACAPB010000402          544 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.450, whole genome shotgun sequence.
ACCESSION   DACAPB010000402 DACAPB010000000
VERSION     DACAPB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.450"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>544
                     /locus_tag="IT904_004792"
     CDS             <1..>544
                     /locus_tag="IT904_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HAP3194438.1"
                     /translation="AGIGAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGD
                     QIFMNEVFLKRLTAPTITSGGNPPVFSLTPDGRLTAKNADISGNVNANSGTLNNVTIN
                     ENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIP
                     AVAFSGAKHERENNDIYSSCR"
BASE COUNT          133 a          141 c          158 g          112 t
ORIGIN      
        1 gcgggtattg gtgccggtat tgagaacacc cctgacggca tgcagagtca ggtgctgctg
       61 gcagcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc gatgtttgtt
      121 gggcagggcg atcagatatt catgaacgaa gtgttcctga aacgcctgac ggctcccacc
      181 attaccagcg gcggtaatcc tccggtattt tccctgacac cggacgggcg gctgacggcg
      241 aaaaatgccg atatcagcgg taacgtgaat gcgaactccg ggacgctcaa caacgtcacg
      301 attaacgaga actgtcgggt tctgggaaaa ctgtccgcca accagattga aggcgatctc
      361 gttaaaacag tgggcaaagc tttcccccgg gactcccgtg caccggaacg gtggccatca
      421 gggaccatca ccgtcagggt ttatgacgat cagccgtttg accggcagat tgttattccg
      481 gcggtggcat tcagcggcgc taaacatgag cgggagaata acgatattta ttcgtcatgc
      541 cgcc
//
LOCUS       DACAPB010000403          516 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.458, whole genome shotgun sequence.
ACCESSION   DACAPB010000403 DACAPB010000000
VERSION     DACAPB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.458"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>516
                     /locus_tag="IT904_004793"
     rRNA            <1..>516
                     /locus_tag="IT904_004793"
                     /product="16S ribosomal RNA"
BASE COUNT          134 a          116 c          170 g           96 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgc
//
LOCUS       DACAPB010000404          512 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.459, whole genome shotgun sequence.
ACCESSION   DACAPB010000404 DACAPB010000000
VERSION     DACAPB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.459"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..>512)
                     /gene="sitC"
                     /locus_tag="IT904_004794"
     CDS             complement(<1..>512)
                     /gene="sitC"
                     /locus_tag="IT904_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAP3194439.1"
                     /translation="PFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGDALSHSI
                     VPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFFGLGLFM
                     VSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFFDENHAR
                     AIGLHPGRLK"
BASE COUNT          157 a          130 c          129 g           96 t
ORIGIN      
        1 atttttaatc tgcccggatg caatccaata gcacgggcgt gattctcgtc gaaaaaggtc
       61 accataagat ctttccattt gaaaaaaagc acgatgatcg agagaatgcc aatgatagtg
      121 agttgcagga tatccgccgg atcgattgcc agaatattgc ccaggacgat ggtctgaatg
      181 ttcacggatg tcgggttcaa tgacaccata aacagcccca ggccaaaaaa ggacgagaag
      241 atcaggccga tgatggcatc ttcttttaag cgagtgagtt ggttaagaaa cagcatgctg
      301 cccgctgcca gtccgccgga aaagaaggct cccagagaaa acggaagccc cagcatatat
      361 gcccccgcaa cacccgggac aatagagtgc gacagcgcat cgccaatcag cgaccagcct
      421 tttagcatca gatagcatga caggaaagcg cagagaccgc cgaccatcgc cgagacccac
      481 atcgcattga gcatatattc atagctgaag gg
//
LOCUS       DACAPB010000405          512 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.460, whole genome shotgun sequence.
ACCESSION   DACAPB010000405 DACAPB010000000
VERSION     DACAPB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.460"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            74..>512
                     /locus_tag="IT904_004795"
     CDS             74..>512
                     /locus_tag="IT904_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194440.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDR"
BASE COUNT          148 a          101 c          152 g          111 t
ORIGIN      
        1 tgagcagaca gaaccgaact gaacctgaac gggttaaatc accgtcacgt ggattttcta
       61 tgggattctg aacatggcca gcacagaaga aaaattactc acgctcctgt tatcggtgga
      121 gcatctgcaa cataccgcaa tggagcaaca gaaagctgtt gctgattctc tggctgtggc
      181 aagggaaaca cttcgcggat atcgggatat ttctgacggt gtggcaaaac aggtcaggga
      241 tgaagtcaga aatgaggtgc agaagctgga cgtggcagga ctgataggtg agcgcatcag
      301 ccaggatttt cagcggctgg aagagtcagt cgcaaacatg aaggcaaacg cggatgccct
      361 gaacagggat attgcgacaa ttcacagcaa tatcgtgcag gaatacaaca cccttaaagg
      421 gtatagctgg aaatggctgg gtggggtgtc agtggctttt ctggttatgt tctgtgtgct
      481 cggatgggga atgaaaacaa tactcgacag ga
//
LOCUS       DACAPB010000406          508 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.462, whole genome shotgun sequence.
ACCESSION   DACAPB010000406 DACAPB010000000
VERSION     DACAPB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.462"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>508
                     /locus_tag="IT904_004796"
     CDS             <1..>508
                     /locus_tag="IT904_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HAP3194441.1"
                     /translation="VTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDII
                     PWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFD
                     PVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAM
                     ERLPAQYQP"
BASE COUNT          121 a          131 c          143 g          113 t
ORIGIN      
        1 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
       61 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      121 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      181 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      241 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      301 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      361 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
      421 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
      481 gagcgcctgc cggcccagta tcagcccg
//
LOCUS       DACAPB010000407          478 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.468, whole genome shotgun sequence.
ACCESSION   DACAPB010000407 DACAPB010000000
VERSION     DACAPB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.468"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..203)
                     /locus_tag="IT904_004797"
     CDS             complement(<1..203)
                     /locus_tag="IT904_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP3194442.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEV"
     gene            389..478
                     /locus_tag="IT904_004798"
                     /pseudo
     CDS             389..478
                     /locus_tag="IT904_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226965.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          123 a          123 c          111 g          121 t
ORIGIN      
        1 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
       61 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
      121 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacca
      181 aaaaaaatat tagttcgatt caatggaggt tccttcagtt ttctgatgaa gcgcggaggt
      241 ggctcaacct gcgaaaagaa acgagttgct atggacttgc accggttgtg ttccggtcta
      301 tctctcattt taagcggctt ttttctcaaa tgccaccggc gatttccagc cgagtgttga
      361 atgtcttcgg cgtggattat aaaagccatt tacccatata atgcgcctac ttatcagcct
      421 ttgggacgtt gggacggtat ttttcactat atacccccaa gtttttagac atgaaaaa
//
LOCUS       DACAPB010000408          477 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.469, whole genome shotgun sequence.
ACCESSION   DACAPB010000408 DACAPB010000000
VERSION     DACAPB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.469"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..431
                     /locus_tag="IT904_004799"
     CDS             <1..431
                     /locus_tag="IT904_004799"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     glyceraldehyde 3-phosphate from fructose 1,6,
                     bisphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP3194443.1"
                     /translation="TIAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLN
                     FVFHGGSGSTAQEIKDSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPK
                     GEDQPNKKYYDPRVWLRAGQTSMIARLEKAFQELNAIDVL"
BASE COUNT          124 a          125 c          116 g          112 t
ORIGIN      
        1 tcaccatcgc tgcgtccttc ggtaacgtac acggtgttta caagccgggt aacgtggttc
       61 tgactccgac catcctgcgt gattctcagg aatatgtttc taagaaacac aacctgccgc
      121 acaacagcct gaacttcgta ttccacggtg gttccggttc tactgctcag gaaatcaaag
      181 actccgtaag ctacggcgta gtaaaaatga acatcgatac cgatacccaa tgggcaacct
      241 gggaaggcgt tctgaactac tacaaagcga acgaagctta tctgcagggg cagttgggta
      301 acccgaaagg cgaagatcag ccgaacaaga aatactacga tccgcgcgta tggctgcgtg
      361 ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg
      421 acgttctgta agatattcct ttctgcttat ctcaaggccc gctctgcggg tcttttt
//
LOCUS       DACAPB010000409          472 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.471, whole genome shotgun sequence.
ACCESSION   DACAPB010000409 DACAPB010000000
VERSION     DACAPB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.471"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            1..472
                     /gene="tsf"
                     /locus_tag="IT904_004800"
                     /pseudo
     CDS             1..472
                     /gene="tsf"
                     /locus_tag="IT904_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
BASE COUNT          131 a          105 c          136 g          100 t
ORIGIN      
        1 cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact
       61 gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat
      121 taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga
      181 cggcaactac ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc
      241 tggtttccag gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga
      301 cgttgaagtt ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg
      361 taaaacatca atattcgccg cgttgctgcg ctggaaggcg acgttctggg ttcttatcag
      421 cacggtgcgc gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga gc
//
LOCUS       DACAPB010000410          451 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.478, whole genome shotgun sequence.
ACCESSION   DACAPB010000410 DACAPB010000000
VERSION     DACAPB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.478"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..348
                     /locus_tag="IT904_004801"
     CDS             <1..348
                     /locus_tag="IT904_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194444.1"
                     /translation="KLGEQTARQRQIIDLLDNEAGLTEQERKLLHVLATAEKNDLQAQ
                     ESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTGELCYKKDSILQALKE
                     IKYDLETSPNPDA"
BASE COUNT          143 a          125 c          110 g           73 t
ORIGIN      
        1 aagctcggag aacagaccgc acgacagaga cagattattg acctgctcga caacgaagca
       61 ggactcacag aacaggaacg caaactcctc cacgtactgg caacagcgga aaaaaacgac
      121 ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga gggaaaaaaa
      181 caacgacgag aacgcaatca ccgactcttt ctcgcggctg ggctactgat cgaagcagga
      241 ctggtagaca caaaaacagg cgagctatgc tataaaaaag acagtatatt acaggcactt
      301 aaggaaatta agtacgatct ggagacatcc ccaaacccag acgcgtaagt gcctccatcc
      361 gtgttccggt tcacttccgc ttcctcgccc actcgctcac tacgttcggt cgctggggct
      421 gcggcgagcg gagtgatctc tctctcgatc c
//
LOCUS       DACAPB010000411          447 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.479, whole genome shotgun sequence.
ACCESSION   DACAPB010000411 DACAPB010000000
VERSION     DACAPB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.479"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(18..227)
                     /gene="mokC"
                     /locus_tag="IT904_004802"
     CDS             complement(18..227)
                     /gene="mokC"
                     /locus_tag="IT904_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAP3194445.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(270..>447)
                     /locus_tag="IT904_004803"
     CDS             complement(270..>447)
                     /locus_tag="IT904_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP3194446.1"
                     /translation="FFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGL
                     TPIEYRNQTYMPRV"
BASE COUNT          115 a          113 c           92 g          127 t
ORIGIN      
        1 actccccgcc gttgctctta ctcggattcg taagcagtga aaacagcaac ctccgtctgg
       61 ccagttcgga tgtgaacctc acagaggtct tttctcgtta ccagcgccgc cactacggcg
      121 gtgatacaga tgacgatcag ggcgacaatc atcgccttat gctgcttcat tgctctcttc
      181 tccttgacct tacggtcagt aagaggcact ctacatgtgt tcagcatata gggggcctcg
      241 ggttgtactg accccaaaaa gttggacagt taaacacgag gcatataggt ctgattccga
      301 tattcaattg gagtcagacc ttttaatttc aggctaattc ttctgctgtt gtagtattca
      361 atatattccg taacagcatc cttcagttcg cttatattac tgaactcatc aagataaaaa
      421 cactccgact ttaaggttcc aaagaaa
//
LOCUS       DACAPB010000412          433 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.481, whole genome shotgun sequence.
ACCESSION   DACAPB010000412 DACAPB010000000
VERSION     DACAPB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.481"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..146)
                     /locus_tag="IT904_004804"
     CDS             complement(<1..146)
                     /locus_tag="IT904_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3194447.1"
                     /translation="MIIGYARKSTHLQDVTHQVDELTKAGCEQIYHEQISRGGTKRAK
                     NGAP"
BASE COUNT          102 a           83 c          101 g          147 t
ORIGIN      
        1 tccggggcac catttttggc gcgtttagtg ccgccgcgtg agatctgttc atgatagatt
       61 tgctcgcaac ccgcttttgt cagttcatca acctgatgcg ttacatcctg aagatgtgtc
      121 gatttacgcg catagccgat tatcattttg ccaccctatt ttgttacatc gtttttgttg
      181 tattgatact gtaacgggtt ttgttacagc cggagagcgt tttattgtat aggcaactgc
      241 ttatagggat gtgtcacaga aaccatcgtt tttgatacat taatttaacc aataggttgc
      301 agatcaaatc gcctgtaaca gcctttctgg ctgtttgtat ataattatga aaaaatggtg
      361 agtagagttt cagggtaaca ggggatgttt atgtcggttt tccacaactg gctactcgag
      421 atcgcatgtg aga
//
LOCUS       DACAPB010000413          383 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.denovo.491, whole genome shotgun sequence.
ACCESSION   DACAPB010000413 DACAPB010000000
VERSION     DACAPB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.denovo.491"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..>383
                     /locus_tag="IT904_004805"
     CDS             <1..>383
                     /locus_tag="IT904_004805"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725196.1"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP3194448.1"
                     /translation="LRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLMGYVDLA
                     KFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGAPVTFIKVHHQPDGHFPNGIPNPLLP
                     ECRQDTADAVRAHQADMGIAFDGDF"
BASE COUNT           66 a          118 c          138 g           61 t
ORIGIN      
        1 ctgcgggata tccagcgcct ggcggaggag aaccagttcc cgccggtgga cccggcgcgt
       61 cgcgggaccc tgcgccagat ttcggtgctg aaggagtacg tcgaccacct gatgggctac
      121 gtggacctgg cgaagttcac ccgtccgctg aagctggtgg tgaactccgg caacggggcg
      181 gcggggcacg tgattgatga agtggagaaa cgcttcgcgg cggccggggc gccggtgacc
      241 tttatcaagg tgcatcacca gccggacggc catttcccga acggtatccc gaacccgctg
      301 ctgccggagt gccgccagga caccgccgac gcggtgcgtg cgcatcaggc ggacatgggg
      361 atcgcctttg acggcgactt cga
//
LOCUS       DACAPB010000414         7843 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.195, whole genome shotgun sequence.
ACCESSION   DACAPB010000414 DACAPB010000000
VERSION     DACAPB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.195"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..1395)
                     /gene="pepD"
                     /locus_tag="IT904_004806"
     CDS             complement(<1..1395)
                     /gene="pepD"
                     /locus_tag="IT904_004806"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP3194449.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHQVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHI"
     gene            1656..2114
                     /gene="gpt"
                     /locus_tag="IT904_004807"
     CDS             1656..2114
                     /gene="gpt"
                     /locus_tag="IT904_004807"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP3194450.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2206..3450
                     /gene="frsA"
                     /locus_tag="IT904_004808"
     CDS             2206..3450
                     /gene="frsA"
                     /locus_tag="IT904_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP3194451.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            3508..3909
                     /locus_tag="IT904_004809"
     CDS             3508..3909
                     /locus_tag="IT904_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP3194452.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3948..5003)
                     /gene="phoE"
                     /locus_tag="IT904_004810"
     CDS             complement(3948..5003)
                     /gene="phoE"
                     /locus_tag="IT904_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP3194453.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            5291..6394
                     /gene="proB"
                     /locus_tag="IT904_004811"
     CDS             5291..6394
                     /gene="proB"
                     /locus_tag="IT904_004811"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP3194454.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6406..7659
                     /gene="proA"
                     /locus_tag="IT904_004812"
     CDS             6406..7659
                     /gene="proA"
                     /locus_tag="IT904_004812"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP3194455.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         1888 a         1960 c         2051 g         1944 t
ORIGIN      
        1 gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat
       61 gtccatttcc ggatacggtt ttttaaacag accacattcc aggcccgcgt ggataatctg
      121 gatgttcggc gtcttgttga acaggcgctg ataggtttca cgtacctgat gcatcaccgg
      181 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
      241 cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagtga
      301 acggatcagg cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag
      361 ggaggtttcg accacacctt ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc
      421 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agagcagctt tatcgttcgc
      481 tacagagtcc agcaacaagg ccagattctt ctctttctct gccagctcgt ttttcaggat
      541 gtcctgatag gtattcacca gagatttcag ggcgtcgact ttatcagctg cgacagcaat
      601 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccaccgttga aatcgataag
      661 gcgcaggtcc agctcttccg catgacccgc caggaagcgc accagcagtt tgttggcatt
      721 acccaggcca acgtggattt caccgccgga gtgaccgcct tttagacctt ttaaggttaa
      781 cttgaaggtt tcaaagccag ctggaaccgc ttcacgatcc aaatgcaggt tggaggtgaa
      841 gtcgataccc cccgcgcaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
      901 cagaatatca gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc
      961 ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac
     1021 cgccagcgca gacgccatac caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt
     1081 aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc
     1141 gttatttttc tgcggcacca tatcgaggtg ggcctgcaag acgaccggtt tacgattttc
     1201 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
     1261 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     1321 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     1381 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     1441 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac
     1501 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     1561 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     1621 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     1681 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     1741 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
     1801 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     1861 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     1921 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     1981 ttgtcaccat ctttgcaaaa ccggctggtc gtccgctggt tgatgattat gttgttgata
     2041 tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     2101 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     2161 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     2221 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     2281 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     2341 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     2401 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     2461 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cacccaggcg
     2521 atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg tcgtcactgg
     2581 ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg
     2641 gccgagcaag cgcaggcttt gtcaaaccgc gcctatgaag aggccgccca gcgtctgccg
     2701 ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg
     2761 catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg
     2821 atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg
     2881 ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc
     2941 agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact
     3001 cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt
     3061 gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt
     3121 gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc cagtcgtttg
     3181 gggatgcatg atgcttccga tgaagcgttg cgcgtggagc tgaatcgcta ttcattaaaa
     3241 gtgcaaggat tgctgggacg tcgctgccca acgccaatgt tatcaggcta ctggaagaac
     3301 gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa
     3361 ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc
     3421 acagactgga tcgaaaaacg tttgtgttaa aaatttgcta aattttgcca atttggtaaa
     3481 acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga
     3541 ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat
     3601 cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt
     3661 gagttctggg gctggtggat ggagcttgaa gcgcaggaat cccgttttac ctacagttac
     3721 cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa agacactgaa
     3781 gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg
     3841 acgacgctga atctgaagct ggaaccggcg gatgattttc gtgatgaacc ggtgaagtta
     3901 acggcgtgag tgaaatgtgc cggatgtatc acatccggca atatccatta aaactgatag
     3961 gtcatgccaa ccgcgacaat atcatcatta ttaatattca atttgttatc gctatccagt
     4021 tggttgattt tataatctac aaacgctgac atatttttgt tgaaataata cgtagccccg
     4081 acatcgatat aattgaccag atcttcatca ccgatacctt caatatcttt ccctttcgat
     4141 aagacataac ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca
     4201 aagttctgtg tcttattggc aaagccgcca gttattggcg tcattttgcg tgtttcagaa
     4261 taaaaagttg ccagataaat attattggca tcgtatttca gacctgtagc ccatgcttct
     4321 gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta
     4381 taggccccac taatggcgaa atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag
     4441 ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt
     4501 aagttcaggc catcgataac gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg
     4561 gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt cgccaccaaa ttccgggaac
     4621 atatcggtcc aggcttccac gtcatacaac gcgcccaggt tacgaccata atcgaaagaa
     4681 cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg ttttttgctg tgcagtatca
     4741 ctctctgctt tattaccggc aaactctgct tcccaacgac cataaccagt cagttgatcg
     4801 ttaatttgtg tttcgccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg
     4861 tcgttatcac tcatataatg catggcttta actttgccat agacatccag tttattaccg
     4921 tctttgttat atatttctgc ggcctgtacg gatgcagatg ccacaatgcc catcaccact
     4981 aatgccagag tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattcag
     5041 cgggagagtc ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac
     5101 agattaataa tattcttaaa atgtggtaat ttagtaaatc tgtaataaaa gcgtaaacaa
     5161 ctgccgctag gcttgctgat cccgcgcaac aaaacgccat gctttgctcg cagatggttg
     5221 gcaaccgacg acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg
     5281 gcagagaatc atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac
     5341 aggcggatcg cgccgtctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca
     5401 gttacatgcc gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg
     5461 tgagcacctg ggttacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcggc
     5521 ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca
     5581 cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgagaacgct tcctgaacgc
     5641 ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga
     5701 tgctgtcgct acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc
     5761 gattctggcg ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc
     5821 tgacccgcgc agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc
     5881 actgcgcgcg attgccggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa
     5941 attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag
     6001 caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc
     6061 ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga
     6121 aatcacggta gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc
     6181 gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa
     6241 cctcgaaggc cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg
     6301 tattgccgga caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt
     6361 tgccgttcac cgtgatgaca tgattacccg ttaaggagca ggctgatgct ggaacaaatg
     6421 ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat
     6481 cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct
     6541 aacgcccagg atgttgctga cgcgcgagcc aatggcctta gcgaagcgat gcttgaccgt
     6601 ctggcactga cgcccgcacg gctgaaaggc attgccgacg atgtacgtca ggtgtgcaac
     6661 ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac tggacagcgg tctgcgtctt
     6721 gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg
     6781 acggttgatg tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct gcgcggtggc
     6841 aaagaaacgt gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaaatcc
     6901 tgcggcttac cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt
     6961 gaaatgctgc gtatggataa atacatcgac atgctgatcc cgcgtggtgg cgctggtttg
     7021 cataaactgt gccgtgaaca gtcgacaatc ccggtgatca caggtggtat aggcgtatgc
     7081 catatttacg ttgatgaaag tgtagagatc gctgaagcat taaaagtgat cgtcaacgcg
     7141 aaaactcagc gtccgagcac atgtaatacg gttgaaacgt tgctggtgaa taaaaacatc
     7201 gccgatagct tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacattacac
     7261 gcagatgcag ctgcactggc gcagttgcag gcaggccctg cgaaggtggt tgctgttaaa
     7321 gccgaagagt atgacgatga gtttctgtca ttagatttga acgtcaaaat cgtcagcgat
     7381 cttgacgatg ccatcgccca tattcgtgaa cacggcacac aacactccga tgcgatcctg
     7441 acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc cgctgtttac
     7501 gttaacgcct ctacgcgttt taccgacggc ggccagtttg gtctgggtgc ggaagtggcg
     7561 gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc tggaagcact gaccacttac
     7621 aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa
     7681 gtagccatct gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac
     7741 cgtgattcac gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag
     7801 cgcattcgta atgcgaaggt cgtaggttcg actcctatta tcg
//
LOCUS       DACAPB010000415         5371 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.248, whole genome shotgun sequence.
ACCESSION   DACAPB010000415 DACAPB010000000
VERSION     DACAPB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.248"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            47..838
                     /locus_tag="IT904_004813"
     CDS             47..838
                     /locus_tag="IT904_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA22"
                     /protein_id="HAP3194456.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1008..1340
                     /gene="qac"
                     /locus_tag="IT904_004814"
     CDS             1008..1340
                     /gene="qac"
                     /locus_tag="IT904_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /protein_id="HAP3194457.1"
                     /translation="MKNWLFLAIAIFGEVVATSALKSSHGFTKLVPSVVVVAGYGLAF
                     YFLSLALKSIPVGIAYAVWAGLGIVLVAAIAWIFHGQKLDLWAFVGMGLIVSGVAVLN
                     LLSKVSAH"
     gene            complement(1605..2240)
                     /locus_tag="IT904_004815"
                     /pseudo
     CDS             complement(1605..2240)
                     /locus_tag="IT904_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2242..2517
                     /locus_tag="IT904_004816"
     CDS             2242..2517
                     /locus_tag="IT904_004816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194458.1"
                     /translation="MNIGLFGFTNKHYYLATKTQEKYTKPFGSDGSLSPLDYSVGIGT
                     GLELGHFQFMFGYDYPLSNSSKDPDLSQVRQHNFRASIAYSFRKLKK"
     gene            complement(2520..3311)
                     /gene="sul3"
                     /locus_tag="IT904_004817"
     CDS             complement(2520..3311)
                     /gene="sul3"
                     /locus_tag="IT904_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul3"
                     /protein_id="HAP3194459.1"
                     /translation="MSKIFGIVNITTDSFSDGGLYLDTDKAIEHALHLVEDGADVIDL
                     GAASSNPDTTEVGVVEEIKRLKPVIKALKEKGISISVDTFKPEVQSFCIEQKVDFIND
                     IQGFPYPEIYSGLAKSDCKLVLMHSVQRIGAATKVETNPEEVFTSMMEFFKERIAALV
                     EAGVKRERIILDPGMGFFLGSNPETSILVLKRFPEIQEAFNLQVMIAVSRKSFLGKIT
                     GTDVKSRLAPTLAAEMYAYKKGADYLRTHDVKSLSDALKISKALG"
     gene            complement(3780..4025)
                     /locus_tag="IT904_004818"
     CDS             complement(3780..4025)
                     /locus_tag="IT904_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAP3194460.1"
                     /translation="MKIEIMEYNPDWTKNFEEEKIKLLHFFGSHAVAIEHIGSTAIPN
                     QRAKPVIDIFIGVSPFAELPFISAFLMQRSITTLRQI"
     gene            complement(4063..4926)
                     /locus_tag="IT904_004819"
     CDS             complement(4063..4926)
                     /locus_tag="IT904_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP3194461.1"
                     /translation="MIPNSENKRVWFITGASKGLGYAFTCAALKAGDKVVAVARTIDN
                     LAKLEETYQESLLPLNLDVTDREAVFSTVETAVKHFGRLDIVVNNAGIMTMGMIEELN
                     ESDARKLMDTNFFGALWVCQAVMPYLRSQRSGHIIQITSIGAIISGPMSGIYSASKFA
                     LEGMSEALAKEAEHFGVKLTMVEPGGYWTDLYTSMSYSNPLDSYGTLRDELAKQYSED
                     SVDSDPSLAAEALMKLVASNNPPLRLILGSMVYDLAMDTLKARMATWEEWEAVSRASE
                     KAIPAPERYGV"
     gene            complement(5072..5311)
                     /locus_tag="IT904_004820"
     CDS             complement(5072..5311)
                     /locus_tag="IT904_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3194462.1"
                     /translation="MAYVQESIAPEMMGKVFSLLMTAMTLSMPIGLLVAGPVVEVIGV
                     NTWFFWSGVALIVNAVLCRILTRRYDKVTMKPQVD"
BASE COUNT         1460 a         1301 c         1118 g         1492 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta gacatcatga gggtagcggt
       61 gaccatcgaa atttcgaacc aactatcaga ggtgctaagc gtcattgagc gccatctgga
      121 atcaacgttg ctggccgtgc atttgtacgg ctccgcagtg gatggcggcc tgaagccata
      181 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
      241 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
      301 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
      361 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
      421 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
      481 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
      541 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
      601 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
      661 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
      721 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctccc gcgcagatca
      781 gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
      841 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
      901 ccagatttgg cgttgcgtat gctcacagaa aatcgacagc cgcaagactg tttttggcaa
      961 ctcatagcca ccactattta tccttcatac cgtagaggag attgcacgtg aagaactggc
     1021 tctttctggc tattgcaata tttggtgagg tcgtcgcaac ttccgcactg aagtccagcc
     1081 atggattcac caagttagtt ccttctgttg tagttgtggc tggctacggg cttgcgttct
     1141 atttcctctc tctcgcactc aagtccatcc cggtcggcat tgcttatgct gtttgggctg
     1201 gcctcggcat cgtacttgtg gcagctatcg cttggatctt ccatggccag aaactagact
     1261 tgtgggcgtt cgttggcatg ggacttatcg ttagtggcgt cgccgttcta aatctgctat
     1321 ccaaggtcag cgcacattga tcgggctggc atctaacagt tcattcaagc cgacgccgct
     1381 tcgcggcgcg gcttaattca ggtgttagtc ggacgcctac ggcgccgagg aaaaaagaaa
     1441 ccaaaccatt cttgctgcca aagagaaaaa ccccttacgg tggcgtactg ggaaagtgcg
     1501 tatctacgac gccttgccgg tctcaatgtc atgccgtcct ctttcgaagg ctgcttgatg
     1561 caaacctctc aaaaatctgg cataaactat cttgacatat tccgattgaa caccttccga
     1621 atatcagcgg ctatcagcgg gatctcatct tttttcgtga cgtaggagtg ggcattctgc
     1681 tgcagatgaa actggcagcg ttgccacaga attccaggga agacagcatc gattgccgcg
     1741 cgcagtcctg agtggtcatc actggtgatc attcgcaggc cgtgcatccc tcgtcgtacg
     1801 agaccctcga gaaaggaacg ccagtttacc tctgcctcac tgttcgcgac ttcggcgtca
     1861 agaatatgcc gattcccgct gtaatcaacg ccaatagcca ctagaagcgc ctgcttgacc
     1921 acatgggaac cgacgcgaac cgattcatag gtcgcatcaa gtaccaagta ttgaatctgt
     1981 ccgacaggct gcgccttcca agacgtaatc tcttcatcga gctcctttgc cagcctgctg
     2041 acctgggacg agctgacttc ggtgccacaa agaatttcga cgatatccga gaccctgcgg
     2101 gtacttactc ctttgacata catttcggcg atggcgagct tgagagcacg ctcactgcgc
     2161 atgcctttct cgatgcagct gggataaaac tccatcccgc gaacttgtgg tactttcaag
     2221 aggactttcc cggtcgcaag attgaatatt ggattatttg gatttaccaa caaacactac
     2281 tatttagcta cgaaaactca agagaagtac acaaaacctt ttggaagtga tggaagttta
     2341 agtcctcttg attatagtgt aggtattgga acaggtttag aattaggaca tttccagttt
     2401 atgtttggct atgattaccc tcttagtaac agttcgaaag atccagacct aagtcaagta
     2461 cgccaacaca acttcagagc aagtatagca tattcattta gaaagctaaa aaaataaaac
     2521 taacctaggg ctttggatat tttcaaggca tctgataaag acttaacatc atgggtgcgg
     2581 agataatctg cacctttttt gtatgcatac atttctgctg caagagttgg tgctaaacga
     2641 gatttcacat cggttccagt tattttacct aagaatgatt tccgtgacac tgcaatcatt
     2701 acttgcaaat taaaagcttc ttgaatttca gggaaacgct tcaaaacaag aatagatgtt
     2761 tctggattag agcctaaaaa gaagcccata cccggatcaa gaataattcg ttcacgcttt
     2821 acaccagcct caactaaagc agcaattctt tctttaaaaa attccatcat ggaagtaaaa
     2881 acctcttccg gattcgtttc aactttagta gctgcaccaa ttcgctgaac ggagtgcatc
     2941 aacacaagtt tgcaatctga ctttgccaag cctgaataaa tctcaggata aggaaaacct
     3001 tgaatatcat taataaaatc aaccttttgt tctatgcaaa aactctgaac ctcaggttta
     3061 aatgtatcaa cagaaataga aatgcctttt tcttttaaag ccttaatgac aggtttgagt
     3121 cttttgattt cttccacaac gcccacttca gttgtatcag gattactgga agcggctccc
     3181 aaatcaatca catctgctcc atcttcaacc aaatgcagag catgctcaat tgccttatct
     3241 gtatctaaat aaagtcctcc atcggaaaaa ctatcggtgg ttatatttac gattccaaaa
     3301 atcttgctca tatcattttg ctttgattac aaaagtagca tttatcttaa ttgactaatt
     3361 atcacttacg caaattgaca cattttccct atctttgcag cctaattttt ttgttttaat
     3421 tcattatgaa aactgatagg aactgtcatt tcgagttttt cgcggttttg ggtcaaaatt
     3481 cggttttttc cccgaactac gactgttttc gcaccccgaa caactaatcc aatcagaatt
     3541 ttccggcaac gccgactcca agaagtgtaa ttccagtcta tttctgccgt caacccctga
     3601 ttcgcgttaa tgcgacaaag ctcaaacaca taactccccc acgaagccat ataaactccg
     3661 atttcgaggt gttatcctgt tccgcataaa cttcgttttt gggtattata cctacaccaa
     3721 agcaagaaga ttatgggtcc aaacttcatt tgtatatttt gcaaacaaat atctgcctgt
     3781 catatctgtc ggagtgtggt gatactcctt tgcattaaaa atgcgctgat aaaaggtagt
     3841 tcagcaaaag gcgaaacgcc aataaatata tctataaccg gtttcgcccg ttgattgggt
     3901 atcgccgtgc ttccgatatg ttcaatggct acggcatgag aaccgaaaaa atggagcaat
     3961 tttattttct cttcttcaaa attctttgtc caatccgggt tatattccat aatttcaatt
     4021 ttcatgatag tttcgtcctt gcctcaaaaa ccatgtttgg tattatactc catatctctc
     4081 cggtgcagga atagcctttt ctgatgcacg gctaacagct tcccattctt cccaggtagc
     4141 catccgtgct tttaaggtat ccatcgccaa atcgtatacc atgctgccaa ggataagcct
     4201 caaaggcgga ttattgctgg ctactaattt cataagggct tccgctgcca aggaaggatc
     4261 actatcgacc gaatcttcgg aatactgttt cgccagctca tcgcgcagtg taccgtatga
     4321 atctaatgga ttgctgtaac tcatcgaagt gtataaatct gtccaatatc ctcctggttc
     4381 caccatagtg agtttcactc cgaaatgttc cgcttctttt gctaaggctt cactcattcc
     4441 ttccagcgca aatttacttg cactgtaaat accagacatc ggacccgaga taatagcgcc
     4501 aatgcttgta atctgtatga tatgtccaga acgctgcgac ctcaaatagg gcatcactgc
     4561 ctgacaaacc caaagagctc caaaaaagtt tgtgtccatt agtttccgag catcggattc
     4621 gtttagttct tcaatcatac ccatagtcat gatacccgca ttattaacaa caatatcaag
     4681 cctaccgaaa tgtttaactg ctgtttcaac cgtagaaaaa acagcctccc tatctgtaac
     4741 atcgaggttt aatggcagta agctctcttg atatgtttct tctagcttcg ccaaattatc
     4801 gatagtcctt gcaactgcaa caactttatc cccggctttc aaggcggcgc atgtaaaagc
     4861 atagccaagc cccttgcttg ctccggtaat aaaccatact cttttatttt cgctattagg
     4921 tatcatagat attctccttt aggctattat agaaagatta ttataagcga aattaatacc
     4981 gttaaacgcc atgagcccga caattttgag cactttgttt tgtactccca gaagctcacc
     5041 actacaaatt atcatcaacc cggtcctttt ttcagtccac ttgcggtttc attgttactt
     5101 tgtcatagcg tcgtgtcaga atgcggcaga gaacagcgtt tactatcaac gcaacaccag
     5161 accagaaaaa ccatgtatta acacctataa cctcaacaac cggacctgca acaagtaagc
     5221 ctatcggcat agaaagagtc atggcggtca tcaaaaggga aaacaccttg cccatcattt
     5281 caggggcaat gctttcttga acataagcca taacaggaac attcataaat gggcactgtt
     5341 gcaaagttag cgatgaggca gccttttgtc t
//
LOCUS       DACAPB010000416         5100 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.256, whole genome shotgun sequence.
ACCESSION   DACAPB010000416 DACAPB010000000
VERSION     DACAPB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.256"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            108..1013
                     /gene="mph(A)"
                     /locus_tag="IT904_004821"
     CDS             108..1013
                     /gene="mph(A)"
                     /locus_tag="IT904_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAP3194463.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1010..2248
                     /locus_tag="IT904_004822"
     CDS             1010..2248
                     /locus_tag="IT904_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP3194464.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2248..2832
                     /locus_tag="IT904_004823"
     CDS             2248..2832
                     /locus_tag="IT904_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP3194465.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3325..4089)
                     /locus_tag="IT904_004824"
     CDS             complement(3325..4089)
                     /locus_tag="IT904_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAP3194466.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4207..4272)
                     /locus_tag="IT904_004825"
                     /pseudo
     CDS             complement(4207..4272)
                     /locus_tag="IT904_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4292..4642)
                     /locus_tag="IT904_004826"
     CDS             complement(4292..4642)
                     /locus_tag="IT904_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAP3194467.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(4845..>5100)
                     /locus_tag="IT904_004827"
     CDS             complement(4845..>5100)
                     /locus_tag="IT904_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP3194468.1"
                     /translation="DQTFQRAFKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTV
                     QDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
BASE COUNT          891 a         1577 c         1587 g         1045 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccgg attgaatata accgacgtga
       61 ctgttacatt taggtggcta aacccgtcaa gccctcagga gtgaatcatg accgtagtca
      121 cgaccgccga tacctcccaa ctgtacgcac ttgcagcccg acatgggctc aagctccatg
      181 gcccgctgac tgtcaatgag cttgggctcg actataggat cgtgatcgcc accgtcgacg
      241 atggacgtcg gtgggtgctg cgcatcccgc gccgagccga ggtaagcgcg aaggtcgaac
      301 cagaggcgcg ggtgctggca atgctcaaga atcgcctgcc gttcgcggtg ccggactggc
      361 gcgtggccaa cgccgagctc gttgcctatc ccatgctcga agactcgact gcgatggtca
      421 tccagcctgg ttcgtccacg cccgactggg tcgtgccgca ggactcggag gtcttcgcgg
      481 agagcttcgc gaccgcgctc gccgccctgc atgccgtccc catttccgcc gccgtggatg
      541 cggggatgct catccgtaca ccgacgcagg cccgtcagaa ggtggccgac gacgttgacc
      601 gcgtccgacg cgagttcgtg gtgaacgaca agcgcctcca ccggtggcag cgctggctcg
      661 acgacgattc gtcgtggcca gatttctccg tggtggtgca tggcgatctc tacgtgggcc
      721 atgtgctcat cgacaacacg gagcgcgtca gcgggatgat cgactggagc gaggcccgcg
      781 ttgatgaccc tgccatcgac atggccgcgc accttatggt ctttggtgaa gaggggctcg
      841 cgaagctcct cctcacgtat gaagcggccg gtggccgggt gtggccgcgg ctcgcccacc
      901 acatcgcgga gcgccttgcg ttcggggcgg tcacctacgc actcttcgcc ctcgactcgg
      961 gtaacgaaga gtacctcgct gcggcgaagg cgcagctcgc cgcagcggaa tgagcgaacg
     1021 tcgatatagc ccgctcgcga cgctgttcgc ggcgaccttt ctcttccgga tcggcaacgc
     1081 ggtggcggcc ctcgcgcttc catggttcgt cctgtctcat acaaagagcg cggcctgggc
     1141 gggcgccacg gccgctagca gcgtcatcgc gaccatcatc ggcgcgtggg ttggtggtgg
     1201 cctcgtcgat cggttcgggc gcgcgcccgt cgcattgatc tcgggtgtgg tgggcggcgt
     1261 ggccatggcg agcatcccac tgctcgatgc cgttggcgcc ctctcgaaca ctgggctgat
     1321 cgcttgcgtg gtgctcggtg ccgcgttcga cgcacccggt atggccgcgc aggacagtga
     1381 gctgcccaaa ctcggccacg tcgccgggct ctccgttgag cgcgtctcgt cactgaaagc
     1441 ggtgatcggg aacgtcgcga ttctaggtgg cccggccctt gggggggccg caatcggcct
     1501 gcttggcgct gcgccaacgc tcgggctgac ggcgttctgc tccgtccttg caggtctgct
     1561 cggcgcgtgg gtgcttcccg cgcgtgccgc tcggacgatg accacgacgg cgactctctc
     1621 catgcgcgcc ggcgtcgctt ttctctggag cgaacccctg ctgcgccctc tctttggtat
     1681 agtgatgatc ttcgtgggca tcgttggcgc caacggcagc gtcatcatgc ctgcgctgtt
     1741 tgtagatgca ggacgccaag tagcagagct cgggctgttc tcctcaatga tgggggctgg
     1801 tggtctcctt ggcattgcca ttcatgcgtc ggtcggcgcc cggatatcag cgcagaactg
     1861 gctggcggtg gcattttgtg gctctgcggt gggctcgctt ctgctttcac agttgccagg
     1921 cgtgccggtg ctgatgttgt tgggcgcgct cgtgggactg ctgaccggct cagtctctcc
     1981 cattctcaac gctgccatct acaaccgcac gccgccagaa cttctcggcc gggtactcgg
     2041 cacggtctcg gcggtgatgc tgtcagcctc gcccatggtt atgcttgcgg ccggcgcgtt
     2101 tgtcgacctt gctggtccgc tccctggcct cgttgtatcg gccgtgtttg cggggctcgt
     2161 ggctctactc tcgctccgtc ttcaatttgc tacaatggcg gcggcagcca cagcctccgc
     2221 cccaacccat acagaaggtg aacactgatg ccccgcccca agctcaagtc cgatgacgag
     2281 gtactcgagg ccgccaccgt agtgctgaag cgttgcggtc ccatagagtt cacgctcagc
     2341 ggagtagcaa aggaggtggg gctctcccgc gcagcgttaa tccagcgctt caccaaccgc
     2401 gatacgctgc tggtgaggat gatggagcgc ggcgtcgagc aggtgcggca ttacctgaat
     2461 gcgataccga taggcgcagg gccgcaaggg ctctgggaat ttttgcaggt gctcgttcgg
     2521 agcatgaaca ctcgcaacga cttctcggtg aactatctca tctcctggta cgagctccag
     2581 gtgccggagc tacgcacgct tgcgatccag cggaaccgcg cggtggtgga ggggatccgc
     2641 aagcgactgc ccccaggtgc tcctgcggca gctgagttgc tcctgcactc ggtcatcgct
     2701 ggcgcgacga tgcagtgggc cgtcgatccg gatggtgagc tagctgatca tgtgctggct
     2761 cagatcgctg ccatcctgtg tttaatgttt cccgaacacg acgatttcca actcctccag
     2821 gcacatgcgt aaacggaggt gtgcagagtc cctgcggcag gcgacgaaca cgaccgtcgt
     2881 cgattagtac cggtacggtc ggtggtatcg aagtcttgat caccactcag gtctacggct
     2941 tacaaatggt gaccatcccg atacttgcgt cagagcaccg ggccgattct ttgacagtga
     3001 atcactcccg taaggttgtg ccggtgtggg tgtcccgggt cgagacgata ctccgccaat
     3061 gcgcccagca aacaacctgg ccatcgcagg tggtggggag cggtgtggcg gatgagttgg
     3121 acaagttggt gtagcagcac gagcacggcg agataacatc gcaggagttc gacatgctca
     3181 agagacagct gattgcgaat cgcgatgcag attcataacc cgattgcggg ttggcttcac
     3241 tccaccatca ccgagcagac tagcacggcg ggctctgttg caaagattgg cggcagtcag
     3301 aggtaggctg tcgctctgcg ccgatcaggc ggctgctgcg aaatggtggt tgagcatgcc
     3361 catggcctcc gtcagcgccg agggcccaat gccaaaagct ctctccacaa ggcgcacctc
     3421 gcccctgatg ccgggctgca ggcaccaggg gcgagcctgt cctttgcgca gggctcgcat
     3481 gacttcgaat cccttgatcg tggcataggc cgtggggatc gatttgaaac cgcgcaccgg
     3541 cttgatcagt atcttgagct ttccgtgatc ggcctcgatc acgttattga gatacttcac
     3601 ctgccggtgg gccgtctccc ggtccagctt tccttcgcgc ttcaattcgg tgatcgctgc
     3661 accatagctc ggcgctttgt cggtattgag cgtggcaggc ttttcccagt gcttcaggcc
     3721 tcgcagggcc ttgcccagga accgcttcgc tgccttggcg ctgcgggtcg gcgacaggta
     3781 gaaatcgatc gtgtcgcccc gcttgtcgac tgcccggtac aggtaggtcc acttgccccg
     3841 caccttgacg taggtttcat ccaggcgcca gctcggatca aagccacgcc gccagaacca
     3901 gcgcagccgc ttctccatct ccggggcgta gcactggacc cagcgataga tcgtcgtatg
     3961 gtcgaccgaa atgccgcgtt ccgccagcat ttcctcaagg tcgcgatagc tgatcggata
     4021 gcgacaatac cagcgcaccg cccacaggat cacatcaccc tggaaatggc gccacttgaa
     4081 atccgtcatc gttccgtccg tccaatctcc gccaagcatg ctcaagcttc acgatttttg
     4141 caacagagcc cacacgagta ttgagcatag tcgagattgg tgcagatcac ttctgatatt
     4201 gaactgtcag gagctggctg cacaacagcc attacgccca atcaactggt gcagtcgtct
     4261 tctgaaaatg acaatccagt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt
     4321 ccggtttgac gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact
     4381 ccagcccgca gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg
     4441 ggatttcctt gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt
     4501 tcctccattg gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga
     4561 aaccctgcac cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca
     4621 caccctcggc taccacctcc attgtcgttt tcagaagacg gctgcactga acgtcagaag
     4681 ccgactgcac tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg
     4741 ccatcagcgg acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc
     4801 ttcgcgcagg ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg
     4861 aggggcggca gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc
     4921 gtgtaaatca tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg
     4981 tcgtaaccgc tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg
     5041 ggcttcgtga tgcctgcttg ttctacggca cgtttgaagg cgcgctgaaa ggtctggtca
//
LOCUS       DACAPB010000417         4870 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.261, whole genome shotgun sequence.
ACCESSION   DACAPB010000417 DACAPB010000000
VERSION     DACAPB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.261"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            89..865
                     /locus_tag="IT904_004828"
     CDS             89..865
                     /locus_tag="IT904_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IV"
                     /protein_id="HAP3194469.1"
                     /translation="MQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGL
                     IEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPF
                     AFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMA
                     KVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARL
                     IRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     gene            1094..2119
                     /gene="aph(4)-Ia"
                     /locus_tag="IT904_004829"
     CDS             1094..2119
                     /gene="aph(4)-Ia"
                     /locus_tag="IT904_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /protein_id="HAP3194470.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE"
     gene            2125..2490
                     /locus_tag="IT904_004830"
                     /pseudo
     CDS             2125..2490
                     /locus_tag="IT904_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
     gene            complement(2541..3293)
                     /locus_tag="IT904_004831"
     CDS             complement(2541..3293)
                     /locus_tag="IT904_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP3194471.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRD
                     LEEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRY
                     LYRAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKT
                     SVDDGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLW
                     LRKGFGFSGGWTVNDQNDLLARLFGLQKVNKA"
     gene            3379..4870
                     /locus_tag="IT904_004832"
                     /pseudo
     CDS             3379..4870
                     /locus_tag="IT904_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT         1003 a         1446 c         1425 g          996 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact
       61 gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat
      121 cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt
      181 ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc
      241 gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt
      301 cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg
      361 cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc
      421 agagcagatc atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc
      481 ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac
      541 gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac
      601 cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga
      661 gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt
      721 cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca
      781 actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga
      841 tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg
      901 caaggtcgcg ctgattgctg gggcaacacg tggagcggat cggggattgt ctttcttcag
      961 ctcgctgatg atatgctgac gctcaatgcc gtttggcctc cgactaacga aaatcccgca
     1021 tttggacggc tgatccgatt ggcacggcgg acggcgaatg gcggagcaga cgctcgtccg
     1081 ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     1141 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     1201 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     1261 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     1321 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     1381 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     1441 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     1501 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     1561 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     1621 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     1681 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     1741 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     1801 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     1861 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     1921 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     1981 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     2041 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     2101 tccgagggca aaggaataga gtagatgccg accgaacaag agctgatttc gagaacgcct
     2161 cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag agaacggcct tacgcttggt
     2221 ggcacagttc tcgtccacag ttcgctaagc tcgctcggct gggtcgcggg agggccggtc
     2281 gcagtgattc aggcccttct ggattgtgtt ggtccccagg gcacgattgt catgcccacg
     2341 cactcgggtg atctgactga tcccgcagat tggagatcgc cgcccgtgcc tgccgattgg
     2401 gtgcagatct tgcgcaacga gatgccggca tacgatccgc aaaccacacc aactcggaac
     2461 atgggtgcag tggctgaatt gttccgggcg tgtcgcaaac acttttggag gcagctatca
     2521 cccagccaag gccggtattt tcatgctttg ttaacctttt gcagtccgaa gaggcgcgca
     2581 agcaaatcat tctggtcatt gacggtccag ccgcctgaaa agccaaagcc cttccgcagc
     2641 cacagcatcg cctcgaaacc cgcgatggtt cgccgcgccg tgttaaagga ttggaaaccg
     2701 ccgatcttcg gcatgttctt cttcacccgg aaatggtcgc tctcaatccc ctgctggaga
     2761 tgtttggtca cataatgcac gggatcggga tggagaagcc catcatcaac cgacgttttg
     2821 atcgttgacg ggaaggtgtt ggccccgtcc gtcccaatcc tgttcggcga caacaagggt
     2881 tcatctttaa gcatctttcg gaagaaccgc ttggcagcgt cgagatcgcg cttagcggtc
     2941 agcaggaaat ccaccggatt gccatgctta tcgatggctc ggtacagata gcgccatttg
     3001 ccgcggatct tgacataggt ctcatcaatc cggaccgagc cgcaatgggg tcgacgaaac
     3061 tgccgcagcc gtttctcgat gacaggtgca taagccaata cccagcggtt gatcgtgcta
     3121 tgatcgacct cgaagccccg ctcgcgaaac atctcttcca agtcacgata gctgagcggg
     3181 tagcgcaagt accaggcaac cgcctgtaca atcagccagg cctcgaaatg cctgcccttg
     3241 aaatcatcct tcgactggcg cttcagcttt tcggcaatgg tattcaaaat catcggctcg
     3301 ctccgcaata tcaggagcgg caacttctct ccccatggtc aacatcgagt taacctgaag
     3361 aatttgcgac aaggccatgc gttccgctcc ggagatgtct ggcttactag gtcccggcgc
     3421 tatggcgatc tgaaacacgc actcgttccg gcacaatcca tcgcggaagg cggtcgtctc
     3481 gctgtgccat tgcggccgga ggaatggctg gcagaccggc aagctcgcct cgacatgcgg
     3541 ttgcgcgagc ttggccgtgc cgctcgcgca ggcacgatcc cgggcgggtc gattgaaaac
     3601 ggcgttctgc atatcgagaa actcgaagcc gccgcgccga caggcgccga agatctggtg
     3661 ctcgatctct acaagcagat cccgcccacg cgcatcaccg atctcctgct ggaggtggat
     3721 gcggcgaccg gcttcaccga agcgttcacc catctgcgca caggagcacc ctgcgctgac
     3781 cggatcgggc taatgaacgt tatcttggcg gaagggatca acctcggctt gcgcaaaatg
     3841 gcggatgcga caaacaccca caccttctgg gaattgatcc gcattggacg gtggcatgtc
     3901 gagggcgaag cctatgaccg ggcgctggcc atggtggtcg aggcacaggc agcgttaccc
     3961 atggcccggt tctggggcat gggcacgtcg gcttcgagcg acggacagtt cttcgtcgct
     4021 acagagcaag gtgaggccat gaacctggtc aacgcgaaat atggcaatac cccgggcctg
     4081 aaagcctata gccacgtctc cgaccaatat gcgccgttcg caacccaggt gattcctgca
     4141 acggcaagcg aagcgcctta catcctcgat ggcctgctga tgaacgatgc tggacgccat
     4201 atccgcgagc agttcaccga cacgggcggc ttcaccgatc acgtctttgc cgcatgtgcc
     4261 attctcggct accggttcgc tccgcgcatc cgcgacctgc catccaaacg gctctacgcg
     4321 ttcaatccgt cggccgcccc ggcgcacctg cgagcgttga tcggcggaaa ggtcaaccaa
     4381 gccatgatcg agcgcaattg gcccgacatc ctgcgcatcg ccgccaccat tgctgccggg
     4441 accgtcgcgc caagccagat tctgcggaaa ctcgcctcct atccgcggca gaacgagctc
     4501 gcgacagccc tgcgggaagt cggtcgcgtc gagcgcaccc tgttcatgat cgactggatt
     4561 ctggatgccg aactccaacg gcgtgcccag atcgggctca acaaaggcga agctcatcat
     4621 gcgctgaagc gggcaatcag cttccaccgc cgcggtgaaa tccgcgaccg ttccgccgaa
     4681 ggccagcatt accgcatcgc cggcatgaat ctgctcgccg ccatcatcat cttctggaac
     4741 accatgaagc tcggcgaggt cgttgcaaac cagaaacgcg atggaaagct gctatcgccc
     4801 gatctcttgg cccatgtttc gccgctcgga tgggaacaca tcaatctcac cggagaatat
     4861 cgctggccaa
//
LOCUS       DACAPB010000418         4057 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.284, whole genome shotgun sequence.
ACCESSION   DACAPB010000418 DACAPB010000000
VERSION     DACAPB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.284"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            11..1345
                     /locus_tag="IT904_004833"
     CDS             11..1345
                     /locus_tag="IT904_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP3194472.1"
                     /translation="MQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPS
                     CGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIAT
                     HLVKKAGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKA
                     PTSPELTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRI
                     AVGSQAGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISR
                     PAVSEKRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHG
                     VFAPNSRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSG
                     CGGAMKVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            1376..2260
                     /locus_tag="IT904_004834"
     CDS             1376..2260
                     /locus_tag="IT904_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HAP3194473.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRR
                     GVERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDAD
                     QWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLA
                     PTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALS
                     KGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGI
                     VQGGSVSWQLGRQRGLER"
     gene            2477..3691
                     /gene="floR"
                     /locus_tag="IT904_004835"
     CDS             2477..3691
                     /gene="floR"
                     /locus_tag="IT904_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HAP3194474.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTYFVFFSTATRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            3719..4024
                     /locus_tag="IT904_004836"
     CDS             3719..4024
                     /locus_tag="IT904_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP3194475.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
BASE COUNT          755 a         1178 c         1263 g          861 t
ORIGIN      
        1 gccgggctat gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg
       61 ctttctacgg gttcgctgcg agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa
      121 gcgtcgcggt ttctgcccga gctgtggggc gcggcggatg gccgaaagtg ccgccttgct
      181 ggttgatgaa gtactgcctg aacaacccat gcgtcagtgg gtgttgagct tcccgtttca
      241 gctgcgtttc ctgtttgcca gccggcccga gatcatgggg tgggtgctgg gcatcgttta
      301 ccgcgtcatt gccacgcacc tggtcaagaa agcgggccat acccaccaag tggccaagac
      361 gggcgcggtc accctgatcc agcgttttgg atcggcgctc aatctgaatg ttcacttcca
      421 catgctgttt ctcgacggtg tgtatgtcga gcaatcccac ggctcagcgc gtttccgctg
      481 ggtcaaggcg ccgaccagcc cagagctcac ccagctgacg cacaccatcg cccaccgggt
      541 gggtcgctat ctggaacggc aaggcctgct ggaacgggat gtcgaaaaca gctatctggc
      601 ctcggatgcg gtggatgacg acccgatgac acccctgctg gggcactcga tcacttaccg
      661 tatcgctgtc ggttcacagg cggggcgaaa ggtgttcact ttgcaaactc tgccgaccag
      721 tggtgatccg ttcggtgacg ggattggcaa ggtagccggg tccagcctgc acgccggcgt
      781 ggcggccagg gccgatgaac gcaagaagct cgaacggctg tgccggtaca tcagccgccc
      841 ggcggtatcc gagaagcggc tgtcgttaac acgaggcggc aacgtgcgct accagctcaa
      901 gacgccgtac cgggacggca ccacgcacgt cattttcgaa ccattggatt tcattgcaag
      961 gctggccgcc ctggtaccga agcccagagt caacctaacc cgcttccacg gggtgttcgc
     1021 acccaacagt cggcaccggg cgttggtcac gccggcaaaa cggggcaggg gcaacaaggt
     1081 cagggtggct gatgaaccgg caacaccagc acaacggcga gcgtcgatga catgggcgca
     1141 acggctcaag cgtgttttca atatcgacat cgagacctgc agcggctgcg gcggcgccat
     1201 gaaagtcatc gcctgcattg aagaccctat agtgatcaag cagatccttg atcacctgaa
     1261 gcacaaagcc gaaaccagcg ggaccagggc gttacccgaa agccgggcgc caccggctga
     1321 gctgctcctg ggtctgtttg actgacgagc ctgaaggcca acgataccaa tcaaaatgct
     1381 gcgttcacag cgccgcggca gggatccgcc gtgctggttg tcggaaaagg agccgctagt
     1441 gggaaagagg agggtaaatt ttcagcgttg ctggctcccc gtcagccgga ttgggttgca
     1501 tcgcaggggt gtcgaaagag tcaactgcgg tccaaagctg ttggacttgg gtgaaaaggg
     1561 cgtttattct tcctatacgt gccgcgttcc aggcggcgac tatgagggct atgtcgatgc
     1621 ccatgtgcgc cggctggagg cgctacgccg ggccggtatc gtcgagcgga tcgacgccga
     1681 ccaatggcgc atccccgatg atctggtcag ccgtgccgcc gcccatgacg ccggccgaga
     1741 cagtcaggcc agcgttcgcg tcctttcccc ggtcgatctg aacaaacaga tcggatcgga
     1801 cggcgcgacc tggctggacc ggcggctgat ccacggcgag acggccgacc ttgcgccaac
     1861 cggcttcggg caacaagtcc gcgaagccat ggaccagcgc cgcgagcacc atatcgaaca
     1921 gggcgacgcc acccgcagcc gggacagccg cgtcttctac cggcgcaacc ttctcgccat
     1981 cctgcgggag cgcgaggtag ccggcgtcgg atcggatatg gctttgagta agggcctgcc
     2041 gttccgcgcc gccacggacg gcgagagcgt cagcggcaag tttaccggaa ccgtgcatct
     2101 atcgagcggc aagttcgccg tggtcgagaa atcccatgag ttcacccttg tcccgtggcg
     2161 gccgatcatc gaccgccaac tcggccgcga ggttatgggc atcgtgcagg gcgggtcggt
     2221 gtcgtggcag ttagggcggc agagggggct ggaacgctga gtgcgcccat gccgcattgc
     2281 gaagcaaaag ataatcggat aaaatgtagc aattcatatt cgtaagcgtg gagtaatcag
     2341 atgggaaatt ccaagtcagc agacaagtaa gccgcaacaa ccagtattgt tgttgcggcg
     2401 ctctgtaagg ctagtctcat ctgattgctg acgagcagac gtcgcccggt attccttaat
     2461 cgagggttga ttcgtcatga ccaccacacg ccccgcgtgg gcctatacgc tgccggcagc
     2521 actgctgctg atggctcctt tcgacatcct cgcttcactg gcgatggata tttatctccc
     2581 tgtcgttcca gcgatgcccg gcatcctgaa cacgacgccc gctatgatcc aactcacgtt
     2641 gagcctctat atggtgatgc tcggcgtggg ccaggtgatt tttggtccgc tctcagacag
     2701 aatcgggcga cggccaattc tacttgcggg cgcaacggct ttcgtcattg cgtctctggg
     2761 agcagcttgg tcttcaactg caccggcctt tgtcgctttc cgtctacttc aagcagtggg
     2821 cgcgtcggcc atgctggtgg cgacgttcgc gacggttcgc gacgtttatg ccaaccgtcc
     2881 tgagggtgtc gtcatctacg gccttttcag ttcgatgctg gcgttcgtgc ctgcgctcgg
     2941 ccctatcgcc ggagcattga tcggcgagtt cttgggatgg caggcgatat tcattacttt
     3001 ggctatactg gcgatgctcg cactcctaaa tgcgggtttc aggtggcacg aaacccgccc
     3061 tctggatcaa gtcaagacgc gccgatctgt cttgccgatc ttcgcgagtc cggctttttg
     3121 ggtttacact gtcggcttta gcgccggtat gggcacctac ttcgtcttct tctcgacggc
     3181 tacccgtgtg ctcataggcc aagcggaata ttccgagatc ggattcagct ttgccttcgc
     3241 cactgtcgcg cttgtaatga tcgtgacaac ccgtttcgcg aagtcctttg tcgccagatg
     3301 gggcatcgca ggatgcgtgg cgcgtgggat ggcgttgctt gtttgcggag cggtcctgtt
     3361 ggggatcggc gaactttacg gctcgccgtc attcctcacc ttcatcctac cgatgtgggt
     3421 tgtcgcggtc ggtattgtct tcacggtgtc cgttaccgcg aacggcgctt tggcagagtt
     3481 cgacgacatc gcgggatcag cggtcgcgtt ctacttctgc gttcaaagcc tgatagtcag
     3541 cattgtcggg acattggcgg tggcactttt aaacggtgac acagcgtggc ccgtgatctg
     3601 ttacgccacg gcgatggcgg tactggtttc gttggggctg gtgctccttc ggctccgtgg
     3661 ggctgccacc gagaagtcgc cagtcgtcta accgacgact ggtagcaggc ccgctccgat
     3721 gcggcgcact aaccatcgaa acctcgtgaa tgtcggtatc ctgtctggca ggataccgct
     3781 catttccctt gttcagttca tcgccgtcgc cgagcatctg aattttcggc atgcggccaa
     3841 ggcacttggt atcagccagt cgagcgtcag cgcgcgtgtg aaagcgctgg aggataacct
     3901 tggtgtcctg ctatttgagc gccatgcgcg gggcgttcgg ctaacagacg caggcaggca
     3961 cttcatggag cgtgtcacgg cgggtgtcga tcaactcgat cacgcagtga agaccgcgga
     4021 gtgacgggca ctggctggca atgtctagca acggcag
//
LOCUS       DACAPB010000419         3672 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.297, whole genome shotgun sequence.
ACCESSION   DACAPB010000419 DACAPB010000000
VERSION     DACAPB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.297"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            complement(<1..433)
                     /locus_tag="IT904_004837"
     CDS             complement(<1..433)
                     /locus_tag="IT904_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5B family transposase"
                     /protein_id="HAP3194476.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEA"
     gene            632..1444
                     /locus_tag="IT904_004838"
     CDS             632..1444
                     /locus_tag="IT904_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase NDM-5"
                     /protein_id="HAP3194477.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     gene            1448..1813
                     /gene="ble"
                     /locus_tag="IT904_004839"
     CDS             1448..1813
                     /gene="ble"
                     /locus_tag="IT904_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein Ble-MBL"
                     /protein_id="HAP3194478.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     gene            1818..2456
                     /locus_tag="IT904_004840"
     CDS             1818..2456
                     /locus_tag="IT904_004840"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HAP3194479.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVA
                     GALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKP
                     VWKALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALP
                     WGLAGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
     gene            complement(2467..3498)
                     /locus_tag="IT904_004841"
     CDS             complement(2467..3498)
                     /locus_tag="IT904_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation (TAT) pathway signal
                     sequence domain protein"
                     /protein_id="HAP3194480.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSA
                     QATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYR
                     KELLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKA
                     RGLHDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRV
                     QGTEGLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDW
                     FVIHAFVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRK
                     PIFALNDAY"
     gene            complement(3503..3631)
                     /locus_tag="IT904_004842"
                     /pseudo
     CDS             complement(3503..3631)
                     /locus_tag="IT904_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
BASE COUNT          685 a         1089 c         1161 g          737 t
ORIGIN      
        1 ctgcttcggc cagccagcga ttgatggtct tgaacaattg gcgggccagt tgatgctgct
       61 ccagcaggtg gcggaaattc atgatggtgg tgcggtcagg caaggcgcta tccagggata
      121 accgggcaaa cagacgcatg gaggcgattt cgtacagagc atcttccatc gcgccatcgc
      181 tcaggttgta ccaatgctgc atgcagtgaa tgcgtagcat ggtttccagc ggataaggtc
      241 gccggccatt accagccttg gggtaaaacg gctcgatgac ttccaccatg ttttgccatg
      301 gcagaatctg ctccatgcgg gacaagaaaa tctcttttct ggtctgacgg cgcttactgc
      361 tgaattcact gtcggcgaag gtaagttgat gactcatgat gaaccctgtt ccatggctcc
      421 agatgacaaa catgatctca tatcagggac ttgttcgcac cttccttaga gaaatttgct
      481 cagcttgttg attatcatat ggcttttgaa actgtcgcac ctcatgtttg aattcgcccc
      541 atatttttgc tacagtgaac caaattaaga tcatctattt actaggcctc gcatttgcgg
      601 ggtttttaat gctgaataaa aggaaaactt gatggaattg cccaatatta tgcacccggt
      661 cgcgaagctg agcaccgcat tagccgctgc attgatgctg agcgggtgca tgcccggtga
      721 aatccgcccg acgattggcc agcaaatgga aactggcgac caacggtttg gcgatctggt
      781 tttccgccag ctcgcaccga atgtctggca gcacacttcc tatctcgaca tgccgggttt
      841 cggggcagtc gcttccaacg gtttgatcgt cagggatggc ggccgcgtgc tgttggtcga
      901 taccgcctgg accgatgacc agaccgccca gatcctcaac tggatcaagc aggagatcaa
      961 cctgccggtc gcgctggcgg tggtgactca cgcgcatcag gacaagatgg gcggtatgga
     1021 cgcgctgcat gcggcgggga ttgcgactta tgccaatgcg ttgtcgaacc agcttgcccc
     1081 gcaagagggg ctggttgcgg cgcaacacag cctgactttc gccgccaatg gctgggtcga
     1141 accagcaacc gcgcccaact ttggcccgct caaggtattt taccccggcc ccggccacac
     1201 cagtgacaat atcaccgttg ggatcgacgg caccgacatc gcttttggtg gctgcctgat
     1261 caaggacagc aaggccaagt cgctcggcaa tctcggtgat gccgacactg agcactacgc
     1321 cgcgtcagcg cgcgcgtttg gtgcggcgtt ccccaaggcc agcatgatcg tgatgagcca
     1381 ttccgccccc gatagccgcg ccgcaatcac tcatacggcc cgcatggccg acaagctgcg
     1441 ctgagccatg gctgaccacg tcacccccaa tctgccatcg cgcgatttcg atgtgacaga
     1501 ggcgttttat gcgaagctgg gctttgcgac gagttggaag gatcgcggct ggatgatcct
     1561 gcagcgcggc ggtttgcagc tcgaattctt cccctatcct gacctcgacc cagctacgag
     1621 ctcgttcggc tgttgcctgc ggttggatga tctcgatgcc atggtggcat tggtgaacgc
     1681 ggcgggagcc gaggaaaaaa gcaccggctg gccgcgcttc aaagctccgc aactggaggc
     1741 gagcggcctg aggatcggct acctgatcga tcccgactgc acgctggtgc ggctgatcca
     1801 gaaccccgac tgaccgcatg cccgcgaaaa tcaagatttg cgggatcagc acacccgagg
     1861 cgctcgatgc gaccatcgcg gcgcgggcgg actatgccgg gttggtgttc tatccagcgt
     1921 cgccccgtgc ggttacgtcg aatgtcgcgg gcgctttgac atcgcgcgca gctggccaga
     1981 tcgccatggt cggtttgttc gtcgatgcgg atgatgctgt catcgccgac gcactggtgg
     2041 cagccaagct gaacgcgctg cagctgcacg gttcggaatc gcccgaacgc gtggcccagt
     2101 tgcgcgcgcg gtttggcaag ccggtgtgga aggcgctgcc cgtcgccagc gccagcgatg
     2161 tcgcacgcgc cgcagcctat gccggggcgg cggacttgat cttgttcgac gccaagaccc
     2221 ccaaaggcgc gctgcccggc ggcatggggt tggcgttcga ctggtcgctg ctggccggat
     2281 atcgcggtgc cttgccgtgg gggctggcag gcgggctaaa tccgacgaat gttgccgagg
     2341 cgattgcgcg caccggagcg ccgctggtcg atacctccag cggcgtcgaa agcgcgccgg
     2401 gcgtcaagga taccgacaag attaccaatt tcgcctttgc ggtgcgcttg gcctaaatcg
     2461 cgtcgatcaa taggcgtcgt tcagcgcaaa gatcggcttg cgggtgcgcc actgccctcg
     2521 ggtgaagtcg ggaaaatcta acgtgcgatt gccctcagca atcgattgtt ccgacagagg
     2581 cgtgatcgcg ctccaggcca gcgcgtcgta aatgtcgatt ggcatcgggg ccttggcctt
     2641 cagcgcctcg acaaaagcgt ggatcacgaa ccagtccatc ccgccatgcc cggcccctgc
     2701 cgccagatcg gcgtagcgtt tccatagcgg gtgatcgtat ttcgcaaacc agccctcggc
     2761 aggctcccag cggtgcggct gtgggctctt gccctccaga tagatcgact tgttgacgtc
     2821 catccacagc ccctcggtgc cttgcacccg aaagccgaga gaataggggc gcggcagcga
     2881 ggtgtcgtgg cacagcatga tcgtttcacc attagtgcag ccgatcatgg tgttgaccac
     2941 atcacccagt gcgaatttca cctcggcgtt gggatgatcg gcagagccgt tcttgacgac
     3001 ataatcatgc agcccgcgcg ccttacagcc gaagccgcca gcgcccgctt cgcccggcaa
     3061 cgcgaccttc agggtgcggg tctgcggcgg gtagcacacg ccggcatcgg cgcagccctg
     3121 gtacttcacg gtcagggtgg tcgcgctcgc gccggccgcg ggcgtgccgg tgagggtgcc
     3181 gagcaattcc ttgcggtagg tttcgacgtc gccgaagaat tcgtcgcggt aggccttgcc
     3241 cttcggcagc gccatggtcg cgccggtgaa ggcggcatcg gccttgaccg aggtgcggtg
     3301 ccggtacagg taatagccgt cggcgatccg ccagcgcacc tcgatgcggt ccggcgcggt
     3361 ggcctgcgcg gacaggacga agacctcgtc gaccggcggc agttcgaagt cctgggcgac
     3421 ggccgaggtc gcgggcagcg caagcagcag ggcgagcccg gccagccagc ggcgcaggcg
     3481 gatcgtggat gcggtcattg gctcagttta ccggtcggct ctcggcggcc agccattgca
     3541 ggtattcggg caggccggac gcggcttcga ccgcgagcag ctccgggagt tcgtagggat
     3601 gcagttggcg caggcgttcc tgcagggcgg ggggcactgt tgcaaatagt cggtggtgat
     3661 aaacttatca tc
//
LOCUS       DACAPB010000420         2588 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.319, whole genome shotgun sequence.
ACCESSION   DACAPB010000420 DACAPB010000000
VERSION     DACAPB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.319"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            24..500
                     /locus_tag="IT904_004843"
     CDS             24..500
                     /locus_tag="IT904_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP3194481.1"
                     /translation="MCNSAIFTDKASGSSTDREGLDLLRMKVEEGDVILVKKLDRLGR
                     DTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEG
                     RQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            683..1543
                     /locus_tag="IT904_004844"
     CDS             683..1543
                     /locus_tag="IT904_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAP3194482.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1705..2520
                     /gene="sul2"
                     /locus_tag="IT904_004845"
     CDS             1705..2520
                     /gene="sul2"
                     /locus_tag="IT904_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP3194483.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          620 a          643 c          688 g          637 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccat atttaccgat aaggcatccg
       61 gcagttcaac agaccgggaa gggctggatt tgctgaggat gaaggtggag gaaggtgatg
      121 tcattctggt taagaagctc gaccgtcttg gccgcgacac tgccgatatg atccaactga
      181 taaaggaatt tgacgctcag ggcgtggcag tccggttcat tgatgacggg atcagtaccg
      241 acggtgatat ggggcaaatg gtggtcacca tcctgtcggc tgtggcacag gctgaacgcc
      301 ggaggatcct agaacgcacg aatgagggcc gacaggaagc aaagctgaaa ggaatcaaat
      361 ttggccgcag gcgtaccgtg gacaggaacg tcgtgctgac gcttcatcag aagggcactg
      421 gtgcaacgga aattgctcat cagctcagta ttgcccgctc cacggtttat aaaattcttg
      481 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctggt aaatgcttca
      661 ataatattga aaaaggaaga gtatgagtat tcaacatttt cgtgtcgccc ttattccctt
      721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      901 gctatgtggt gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
      961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgctgc
     1081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1201 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     1261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1681 gtcagacccc ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1741 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1801 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1861 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1921 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1981 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     2041 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     2101 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     2161 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     2221 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2281 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2341 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2401 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2461 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2521 ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa ggaacctcca
     2581 ttgaatcg
//
LOCUS       DACAPB010000421         2492 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.323, whole genome shotgun sequence.
ACCESSION   DACAPB010000421 DACAPB010000000
VERSION     DACAPB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.323"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            257..499
                     /locus_tag="IT904_004846"
     CDS             257..499
                     /locus_tag="IT904_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP3194484.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(531..1181)
                     /gene="tetR(A)"
                     /locus_tag="IT904_004847"
     CDS             complement(531..1181)
                     /gene="tetR(A)"
                     /locus_tag="IT904_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAP3194485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1287..2486
                     /gene="tet(A)"
                     /locus_tag="IT904_004848"
     CDS             1287..2486
                     /gene="tet(A)"
                     /locus_tag="IT904_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAP3194486.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
BASE COUNT          419 a          797 c          778 g          498 t
ORIGIN      
        1 gaatatcgct ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgacccctgg
       61 aagccccgta gcgacgcgga gaggggcgag acaagccaag ggcgcaggct cgatgcgcag
      121 cacgacatag ccggttctcg caaggacgag aatttccctg cggtgcccct caagtgtcaa
      181 tgaaagtttc caacgcgagc cattcgcgag agccttgagt ccacgctaga tctatctcat
      241 ctgcgcaagg cagaacgtga agacggccgc cctggacctc gcccgcgagc gccaggcgca
      301 cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc aggagcgcca
      361 gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc agggcatgaa
      421 aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag gggatgttgt
      481 ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca
      541 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
      601 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
      661 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
      721 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
      781 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
      841 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
      901 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
      961 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
     1021 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
     1081 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
     1141 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
     1201 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
     1261 agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc gtaattctga
     1321 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
     1381 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
     1441 atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
     1501 ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc atcatggcga
     1561 cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
     1621 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
     1681 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
     1741 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
     1801 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
     1861 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
     1921 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
     1981 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
     2041 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
     2101 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
     2161 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
     2221 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
     2281 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
     2341 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
     2401 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagaa
     2461 attcttcaaa ttcccgttgc acatagcccg gc
//
LOCUS       DACAPB010000422         2085 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.335, whole genome shotgun sequence.
ACCESSION   DACAPB010000422 DACAPB010000000
VERSION     DACAPB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.335"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            148..963
                     /locus_tag="IT904_004849"
     CDS             148..963
                     /locus_tag="IT904_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HAP3194487.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1117..1992)
                     /locus_tag="IT904_004850"
     CDS             complement(1117..1992)
                     /locus_tag="IT904_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HAP3194488.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
BASE COUNT          499 a          529 c          535 g          522 t
ORIGIN      
        1 tgatccttca actcagcaaa agttcgattt attcaacaaa tccacgttgt gtctcaaaat
       61 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
      121 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg
      181 aggccgcgat taaattccaa cctggatgct gatttatatg ggtataaatg ggctcgcgat
      241 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
      301 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
      361 ctaaactggc tgacggcatt tatgcctctt ccgaccatca agcattttat ccgtactcct
      421 gatgatgcat ggttactcac cactgcgctc cccgggaaaa cagcattcca ggtattagaa
      481 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagcgttcct gcgccggttg
      541 cattcgattc ctgttagtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
      601 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
      661 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
      721 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
      781 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
      841 ctatggaact gcctcggtga attttctcct tcattacaga aacggctttt tcaaaaatat
      901 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
      961 tgatagttag tctttgtaga gacacaaacc tgaaattccg gacaccgcgc ttcaggacat
     1021 ctccctggac gccgatatct ggcagtatcc ggacggtact atcgaacgcc gggccaacgg
     1081 tactcccctg ccctttatga ctgacgaccg gctatttcac ccacgcactt ctccatgaaa
     1141 tttagcatcg tcgcggttaa tgtcgaaaat ttgccacctg acgtggttgg catcgagggt
     1201 gaaaactgcc gtggcgaggg tgttctcctc atccggatcg tcggcagcca gccggtaaat
     1261 tggcagttct gcgtcatggg tatctgagag aatggttttt gccgccgcgg cgtcaaacgg
     1321 aggttgcgtt tcccgccact gcacgaggcg taactgccgc gacgctgaac tgtcggtaac
     1381 gatctgctca acgccagcca gtgccgcatg caccagatgg ttggcatgcc cggttaccgt
     1441 cgtcaggggt agaacggaac agccttgacc ggtggcctca acgctgaaca gccgcgcatc
     1501 gcccagctgc ccgagcgtat ggtgaaacgc cccggatcgc ggctggccgg taagtatagt
     1561 gatggcctcg tcgagggtgt tcgcattcag cgaggcgcga gccaggatct gacgcggtaa
     1621 tccctgcgga cgatacactg cacggatgtt attcaccgtg ttaacgatac ctttttcatt
     1681 taccgcaaag gtgtggccgc aaatcgaccc cggataggca aaactcgtaa agctcagacc
     1741 gcgactcgga gtaatggtag ccagcaagca ttcatcccgt aactgaggaa aaccgtcttc
     1801 attatgcgcg ataatccgct cgcctgcggc gtttaccccg gccaccgtcg tacagccatc
     1861 cgacgttgag cgcaccagat cgccacggca gttccaggca aagacctgat caacagtggc
     1921 ctccagtccc tgcgccagcc cctccagctc ctgccagatg tgcggataat gccgctgcac
     1981 ctcggcgcgc attaaccggg tctcagggga tgcctgcagg gtcatcacgg ggtcgtttgc
     2041 gggagggggc ggaatcctac gctaaggctt tggccagcga tattc
//
LOCUS       DACAPB010000423         1773 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.345, whole genome shotgun sequence.
ACCESSION   DACAPB010000423 DACAPB010000000
VERSION     DACAPB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.345"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            244..1503
                     /locus_tag="IT904_004851"
     CDS             244..1503
                     /locus_tag="IT904_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol efflux MFS transporter CmlA1"
                     /protein_id="HAP3194489.1"
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNAL
                     GTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSA
                     EVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGA
                     LVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLY
                     TLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKW
                     GSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALR
                     GFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVS
                     RVKGSRGQGEHDVVALQSAESTSNPNR"
     gene            1596..>1773
                     /locus_tag="IT904_004852"
     CDS             1596..>1773
                     /locus_tag="IT904_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'') family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HAP3194490.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLD"
BASE COUNT          333 a          447 c          523 g          470 t
ORIGIN      
        1 agccgaccgc gctacgcgcg gcggcttaac tccggcgttg ggcgcacaat aaggctcctt
       61 gcagagttgc ttgaaagttg ttacgattca aattcaatca tgagatagtc agcagatgag
      121 cacttccaag aacgcagaca agtaagccgc agcaaccttc atttttcggt tgttgcggcg
      181 ttctcatgaa tccttttgct ctacgggagc gccgccaaat cctttgttca aggagatggt
      241 ttcgtgagct caaaaaactt tagttggcgg tactcccttg ccgccacggt gttgttgtta
      301 tcaccgttcg atttattggc atcactcggc atggacatgt acttgccagc agtgccgttt
      361 atgccaaacg cgcttggtac gacagcgagc acaattcagc ttacgctgac aacgtacttg
      421 gtcatgattg gtgccggtca gctcttgttt ggaccgctat cggaccgact ggggcgccgc
      481 cccgttctac tgggaggtgg cctcgcctac gttgtggcgt caatgggcct cgctcttacg
      541 tcatcggctg aagtctttct ggggcttcgg attcttcagg cttgtggtgc ctcggcgtgc
      601 cttgtttcca catttgcaac agtacgtgac atttacgcag gtcgcgagga aagtaatgtc
      661 atttacggca tactcggatc catgctggcc atggtcccgg cggtaggccc attgctcgga
      721 gcgctcgtcg acatgtggct tgggtggcgg gctatctttg cgtttctagg tttgggcatg
      781 atcgctgcat ctgcagcagc gtggcgattc tggcctgaaa cccgggtgca acgagttgcg
      841 ggcttgcaat ggtcgcagct gctactcccc gttaagtgcc tgaacttctg gttgtacacg
      901 ttgtgttacg ccgctggaat gggtagcttc ttcgtctttt tctccattgc gcccggacta
      961 atgatgggca ggcaaggtgt gtctcagctt ggcttcagcc tgctgttcgc cacagtggca
     1021 attgccatgg tgtttacggc tcgttttatg gggcgtgtga tacccaagtg gggcagccca
     1081 agtgtcttgc gaatgggaat gggatgcctg atagctggag cagtattgct tgccatcacc
     1141 gaaatatggg ctttgcagtc cgtgttaggc tttattgctc caatgtggct agtgggtatt
     1201 ggtgtcgcca cagcggtatc tgtggcgccc aatggcgctc ttcgaggatt cgaccatgtt
     1261 gctggaacgg tcacggcagt ctacttctgc ttgggcggtg tactgctagg aagcatcgga
     1321 acgttgatca tttcgctgtt gccgcgcaac acggcttggc cggttgtcgt gtactgtttg
     1381 acccttgcaa cagtcgtgct cggtctgtct tgtgtttccc gagtgaaggg ctctcgcggc
     1441 cagggggagc atgatgtggt cgcgctacaa agtgcggaaa gtacatcaaa tcccaatcgt
     1501 tgagagaatg tggcaagcta tcgcccaaca aatcgctgca gccgacccaa aaccgctacg
     1561 cggtttcggt cggctgagct caggcgttaa acatcatgag ggaagcggtg atcgccgaag
     1621 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1681 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1741 atttgctggt tacggtgacc gtaaggcttg atg
//
LOCUS       DACAPB010000424         1565 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.355, whole genome shotgun sequence.
ACCESSION   DACAPB010000424 DACAPB010000000
VERSION     DACAPB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.355"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            7..1140
                     /gene="blaEC"
                     /locus_tag="IT904_004853"
     CDS             7..1140
                     /gene="blaEC"
                     /locus_tag="IT904_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP3194491.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
     gene            1229..>1565
                     /locus_tag="IT904_004854"
     CDS             1229..>1565
                     /locus_tag="IT904_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194492.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPF"
BASE COUNT          411 a          405 c          384 g          365 t
ORIGIN      
        1 gaccctatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt
       61 gctgcccccc aaaaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa
      121 caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc
      181 tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta
      241 ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc tcgaggggaa
      301 atcaagttaa gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaac
      361 gggatcacac tattacatct cgcaacctac accgctggcg gcctgccatt gcaggtgccg
      421 gatgaggtga aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg
      481 gctccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct
      541 gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa cacgtgtctt ccagccactc
      601 aaactcaacc atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga
      661 tatcgcgaag gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga aacttatggt
      721 gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat
      781 atcaacgaca aaacacttca gcaagggata caactggcac aatctcgcta ctggcaaacc
      841 ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtaaa tcctgacagc
      901 atcattaacg gcagtggcaa taaaattgca ctggcagcac gccccgtaaa agcgattacg
      961 cccccaactc ctgcagtacg cgcatcatgg gtacataaaa caggggcaac cggcggattt
     1021 ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tagtgatgct ggctaacaaa
     1081 aactatccca atccagcgag agtcaccgcc gcctggcaga ttctcaacgc tctacagtaa
     1141 aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac
     1201 ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac
     1261 agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtagt
     1321 gaataatttc gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca
     1381 ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atgacggcgg
     1441 cctgaatgtc atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg
     1501 gaaagcgtac tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtcc
     1561 tttct
//
LOCUS       DACAPB010000425         1478 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.356, whole genome shotgun sequence.
ACCESSION   DACAPB010000425 DACAPB010000000
VERSION     DACAPB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.356"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..600
                     /gene="aph(3'')"
                     /locus_tag="IT904_004855"
     CDS             <1..600
                     /gene="aph(3'')"
                     /locus_tag="IT904_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP3194493.1"
                     /translation="INWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAV
                     HSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVR
                     LDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENW
                     AAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            600..1436
                     /gene="aph(6)-I"
                     /locus_tag="IT904_004856"
     CDS             600..1436
                     /gene="aph(6)-I"
                     /locus_tag="IT904_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP3194494.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKELK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          307 a          400 c          428 g          343 t
ORIGIN      
        1 atcaactggc aggaggaaca ggagggtgca tgcttggtga taacggcaat tccgggagta
       61 ccggcggctg atctgtctgg agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa
      121 cttggcgctg ttcacagcct atcggttgat caatgtccgt ttgagcgcag gctgtcgcga
      181 atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg
      241 gacgaggaca agagtacgcc gcagctcgat cttttggctc gtgtcgaacg agagctaccg
      301 gtgcggctcg accaagagcg caccgatatg gttgtttgcc atggtgatcc ctgcatgccg
      361 aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct tgggcggctc
      421 ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga agagaactgg
      481 gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa
      541 gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac ttggggttga
      601 tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg
      661 acaccttttc cagcctcgtt tggaaagttt cattgccaga cgggactcct gcaatcgtca
      721 aggaattgaa acctatagaa gacattgctg atgaactgcg cggggccgac tatctggtat
      781 ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga gaacaatctg atgttgctcg
      841 aatatgccgg ggagcgaatg ctctctcaca tcgttgccga gcacggcgac taccaggcga
      901 ccgaaattgc agcggaacta atggcgaagc tgtatgccgc atctgaggaa cccctgcctt
      961 ctgcccttct cccgatccgg gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc
     1021 aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat tatagccgat caaatgatga
     1081 gcaatgcctc ggaactgcgt gggctacatg gcgatctgca tcatgaaaac atcatgttct
     1141 ccagtcgcgg ctggctggtg atagatcccg tcggtctggt cggtgaagtg ggctttggcg
     1201 ccgccaatat gttctacgat ccggctgaca gagacgacct ttgtctcgat cctagacgca
     1261 ttgcacagat ggcggacgca ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg
     1321 accaggcgta cgcttatggg tgcctttccg cagcttggaa cgcggatgga gaagaggagc
     1381 aacgcgatct agctatcgcg gccgcgatca agcaggtgcg acagacgtca tactagatat
     1441 caagcgactt ctcctatccc ctgggaacac atcaatct
//
LOCUS       DACAPB010000426         1478 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.357, whole genome shotgun sequence.
ACCESSION   DACAPB010000426 DACAPB010000000
VERSION     DACAPB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.357"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            <1..600
                     /gene="aph(3'')"
                     /locus_tag="IT904_004857"
     CDS             <1..600
                     /gene="aph(3'')"
                     /locus_tag="IT904_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP3194495.1"
                     /translation="INWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAV
                     HSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVR
                     LDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENW
                     AAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            600..1436
                     /locus_tag="IT904_004858"
     CDS             600..1436
                     /locus_tag="IT904_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAP3194496.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          306 a          400 c          429 g          343 t
ORIGIN      
        1 atcaactggc aggaggaaca ggagggtgca tgcttggtga taacggcaat tccgggagta
       61 ccggcggctg atctgtctgg agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa
      121 cttggcgctg ttcacagcct atcggttgat caatgtccgt ttgagcgcag gctgtcgcga
      181 atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg
      241 gacgaggaca agagtacgcc gcagctcgat cttttggctc gtgtcgaacg agagctaccg
      301 gtgcggctcg accaagagcg caccgatatg gttgtttgcc atggtgatcc ctgcatgccg
      361 aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct tgggcggctc
      421 ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga agagaactgg
      481 gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa
      541 gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac ttggggttga
      601 tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg
      661 acaccttttc cagcctcgtt tggaaagttt cattgccaga cgggactcct gcaatcgtca
      721 agggattgaa acctatagaa gacattgctg atgaactgcg cggggccgac tatctggtat
      781 ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga gaacaatctg atgttgctcg
      841 aatatgccgg ggagcgaatg ctctctcaca tcgttgccga gcacggcgac taccaggcga
      901 ccgaaattgc agcggaacta atggcgaagc tgtatgccgc atctgaggaa cccctgcctt
      961 ctgcccttct cccgatccgg gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc
     1021 aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat tatagccgat caaatgatga
     1081 gcaatgcctc ggaactgcgt gggctacatg gcgatctgca tcatgaaaac atcatgttct
     1141 ccagtcgcgg ctggctggtg atagatcccg tcggtctggt cggtgaagtg ggctttggcg
     1201 ccgccaatat gttctacgat ccggctgaca gagacgacct ttgtctcgat cctagacgca
     1261 ttgcacagat ggcggacgca ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg
     1321 accaggcgta cgcttatggg tgcctttccg cagcttggaa cgcggatgga gaagaggagc
     1381 aacgcgatct agctatcgcg gccgcgatca agcaggtgcg acagacgtca tactagatat
     1441 caagcgactt ctcctatccc ctgggaacac atcaatct
//
LOCUS       DACAPB010000427         1063 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.384, whole genome shotgun sequence.
ACCESSION   DACAPB010000427 DACAPB010000000
VERSION     DACAPB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.384"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            114..935
                     /gene="lnu(F)"
                     /locus_tag="IT904_004859"
     CDS             114..935
                     /gene="lnu(F)"
                     /locus_tag="IT904_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(F)"
                     /protein_id="HAP3194497.1"
                     /translation="MLQLKMIELFKEGCHEDARIIAALMFGSFAIGEGDEFSDIEFAV
                     FIQDDHFENFDQRSWLNAVSPVAAYFPDDFGHHTALFENGIRGEFHFMRKSDIPVIST
                     WQGYGWFPSLEAAVLLDRSGELSRYASALVGGPPIREGAPLVEGLVLNLISLMLFGAN
                     LLNRGEYARAWALLSKAHENLLKLVRLHEGATDHWPTPSRALEKDISEDSYNRYLACT
                     SSAEPRALCAAYHQTWTWSLELFKSVTEPLNIELPRTVIAQAKRLFNESATPHNK"
BASE COUNT          273 a          260 c          272 g          258 t
ORIGIN      
        1 agccgaccgc gctacgcgcg gcggcttaac tccggcgttg tgctgacgaa aaaaaacaaa
       61 aatctgcgta tacctttcct cataatctgt ggcttcaata aaaggatatt tctatgcttc
      121 aactgaaaat gatcgaactc ttcaaggaag gttgtcatga ggatgcacga ataatcgcgg
      181 cattgatgtt cggctcattt gctatcggag agggtgacga gttctctgat atcgaattcg
      241 cagtgttcat ccaggatgac cattttgaaa atttcgatca gcgctcgtgg cttaatgccg
      301 taagtccggt tgctgcttac tttccggacg acttcggcca ccacaccgca ctttttgaaa
      361 acggcattcg cggtgaattc catttcatgc gaaaatcgga cataccggtc atttccactt
      421 ggcaaggcta tgggtggttt ccctcgcttg aggcggctgt tttgttggac cgatcaggag
      481 agttgtcaag gtacgcaagc gctctcgtgg gcggtccccc gatacgtgaa ggcgcgccgc
      541 tggtggaagg gcttgtgttg aacctcatca gcctgatgct ctttggggcc aatcttttaa
      601 atcggggaga gtacgctcgc gcctgggctt tgctcagcaa agcacatgaa aacctactca
      661 agctggttcg actccacgaa ggggcaacag accactggcc gacaccttca cgcgcgctcg
      721 aaaaggatat ctcggaggac tcgtataatc gctatctggc atgcacaagc agtgcagaac
      781 caagagcact atgtgcagcc tatcatcaaa cgtggacgtg gagtctcgaa ttgttcaaga
      841 gcgtgacaga acctctgaat atcgagcttc cgagaactgt aattgcgcag gcaaaaaggt
      901 tgttcaatga gtctgcgacg ccgcacaaca agtaaatcca gcggacgcat aaaaacgcgc
      961 cgctgatttt gacgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     1021 aagtggcact gttgcaaata gtcggtggtg ataaacttat cat
//
LOCUS       DACAPB010000428          959 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.390, whole genome shotgun sequence.
ACCESSION   DACAPB010000428 DACAPB010000000
VERSION     DACAPB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.390"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            33..836
                     /locus_tag="IT904_004860"
                     /note="aph(3'')-Ib"
     CDS             33..836
                     /locus_tag="IT904_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP3194498.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            836..>959
                     /locus_tag="IT904_004861"
     CDS             836..>959
                     /locus_tag="IT904_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194499.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVK"
BASE COUNT          188 a          254 c          277 g          240 t
ORIGIN      
        1 cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt ttttttggtg
       61 aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt gtttttcgac
      121 gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg
      181 gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca
      241 actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg ggagtaccgg
      301 cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg cagcaacttg
      361 gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt
      421 tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg
      481 aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc
      541 ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc atgccgaact
      601 tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa
      661 cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag
      721 cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc
      781 ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt
      841 catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac
      901 cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaagg
//
LOCUS       DACAPB010000429          959 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.391, whole genome shotgun sequence.
ACCESSION   DACAPB010000429 DACAPB010000000
VERSION     DACAPB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.391"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            33..836
                     /locus_tag="IT904_004862"
                     /note="aph(3'')-Ib"
     CDS             33..836
                     /locus_tag="IT904_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP3194500.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            836..>959
                     /locus_tag="IT904_004863"
     CDS             836..>959
                     /locus_tag="IT904_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP3194501.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVK"
BASE COUNT          188 a          253 c          278 g          240 t
ORIGIN      
        1 cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt ttttttggtg
       61 aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt gtttttcgac
      121 gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg
      181 gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc gaggtgatca
      241 actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg ggagtaccgg
      301 cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg cagcaacttg
      361 gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt
      421 tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg
      481 aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc
      541 ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc atgccgaact
      601 tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa
      661 cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag
      721 cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc
      781 ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt
      841 catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac
      901 cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaagg
//
LOCUS       DACAPB010000430          896 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.400, whole genome shotgun sequence.
ACCESSION   DACAPB010000430 DACAPB010000000
VERSION     DACAPB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.400"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            47..838
                     /locus_tag="IT904_004864"
     CDS             47..838
                     /locus_tag="IT904_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA22"
                     /protein_id="HAP3194502.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVIGK"
BASE COUNT          223 a          224 c          249 g          200 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta gacatcatga gggtagcggt
       61 gaccatcgaa atttcgaacc aactatcaga ggtgctaagc gtcattgagc gccatctgga
      121 atcaacgttg ctggccgtgc atttgtacgg ctccgcagtg gatggcggcc tgaagccata
      181 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
      241 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
      301 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
      361 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
      421 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
      481 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
      541 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
      601 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
      661 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
      721 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
      781 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtaatcg gcaaataatg
      841 tctaacaatt cgttcaagcc gaccgcgcta cgcgcggcgg cttaactccg gcgttg
//
LOCUS       DACAPB010000431          896 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.401, whole genome shotgun sequence.
ACCESSION   DACAPB010000431 DACAPB010000000
VERSION     DACAPB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.401"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            47..838
                     /locus_tag="IT904_004865"
     CDS             47..838
                     /locus_tag="IT904_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /protein_id="HAP3194503.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
BASE COUNT          222 a          223 c          247 g          204 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta gacatcatga gggtagcggt
       61 gaccatcgaa atttcgaacc aactatcaga ggtgctaagc gtcattgagc gccatctgga
      121 atcaacgttg ctggccgtgc atttgtacgg ctccgcagtg gatggcggcc tgaagccata
      181 cagcgatatt gatttgttgg ttactgtggc cgtaaagctt gatgaaacga cgcggcgagc
      241 attgctcaat gaccttatgg aggcttcggc tttccctggc gagagcgaga cgctccgcgc
      301 tatagaagtc acccttgtcg tgcatgacga catcatcccg tggcgttatc cggctaagcg
      361 cgagctgcaa tttggagaat ggcagcgcaa tgacattctt gcgggtatct tcgagccagc
      421 catgatcgac attgatctag ctatcctgct tacaaaagca agagaacata gcgttgcctt
      481 ggtaggtccg gcagcggagg aattctttga cccggttcct gaacaggatc tattcgaggc
      541 gctgagggaa accttgaagc tatggaactc gcagcccgac tgggccggcg atgagcgaaa
      601 tgtagtgctt acgttgtccc gcatttggta cagcgcaata accggcaaaa tcgcgccgaa
      661 ggatgtcgct gccgactggg caataaaacg cctacctgcc cagtatcagc ccgtcttact
      721 tgaagctaag caagcttatc tgggacaaaa agaagatcac ttggcctcac gcgcagatca
      781 cttggaagaa tttattcgct ttgtgaaagg cgagatcatc aagtcagttg gtaaatgatg
      841 tctaacaatt cgttcaagcc gaccgcgcta cgcgcggcgg cttaactccg gcgttg
//
LOCUS       DACAPB010000432          574 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  TPA_asm: Escherichia coli strain hkcpe45
            SAMN15904714-rid10980333.guided.441, whole genome shotgun sequence.
ACCESSION   DACAPB010000432 DACAPB010000000
VERSION     DACAPB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15904714
            Sequence Read Archive: SRR12517474
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000881393.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000881393.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2020 01:39:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 60 of 148
            Pseudo Genes (incomplete)         :: 69 of 148
            Pseudo Genes (internal stop)      :: 37 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15904714-rid10980333.guided.441"
                     /strain="hkcpe45"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Hong Kong"
                     /lat_lon="22.3797 N 114.2017 E"
                     /collection_date="2016-11-01"
                     /collected_by="CUHK"
     gene            57..530
                     /locus_tag="IT904_004866"
     CDS             57..530
                     /locus_tag="IT904_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAP3194504.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          164 a          125 c          141 g          144 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta acccaggatg agaaccttga
       61 aagtatcatt gatggctgcg aaagcgaaaa acggcgtgat tggttgcggt ccagacatac
      121 cctggtccgc gaaaggggag cagctacttt ttaaagcatt gacctacaat cagtggcttc
      181 tggtgggtcg caagacgttt gaatctatgg gcgcactccc caataggaaa tacgcggtcg
      241 ttacccgctc aggttggaca tcaaatgatg acaatgtagt tgtatttcag tcaatcgaag
      301 aggccatgga caggctagct gaattcaccg gtcacgttat agtgtctggt ggcggagaaa
      361 tttaccgaga aacattaccc atggcctcta cgctccactt atcgacgatc gacatcgagc
      421 cagaggggga tgttttcttc ccgagtattc caaatacctt cgaagttgtt tttgagcaac
      481 actttacttc aaacattaac tattgctatc aaatttggaa aaagggttaa caaagcggca
      541 ctgttgcaaa tagtcggtgg tgataaactt atca
//
